LOCUS       DAOMAX010000001        44878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_116_length_44878_cov_4.748187, whole genome shotgun sequence.
ACCESSION   DAOMAX010000001 DAOMAX010000000
VERSION     DAOMAX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 44878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44878
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_44878_cov_4.748187"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PK742_00005"
     CDS             <1..679
                     /locus_tag="PK742_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU74996.1"
                     /translation="VPDAFIHPVSVITVIGFLRLYQLIFNSYNKQQADSLLLNAGIWC
                     GILSLLEPAYILIVVPVVIGFSTIRTLTFRESLVILSGTLVMHFLAVTAMLLFGKSDW
                     YLAAWKNWHIPGQDGWPHWLILIQLALALMFLLAATAYLTQHLNNQLIRFRRNTRVLL
                     VFLLGALLLALILPAGVTAVWWWIAAPAALMISWWWTQMKNQDAAAILHLTLLGLVFV
                     FQYINFA"
     gene            692..1399
                     /gene="purQ"
                     /locus_tag="PK742_00010"
     CDS             692..1399
                     /gene="purQ"
                     /locus_tag="PK742_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQU74997.1"
                     /translation="MRFGVVIFPGSNCDQDIIHVLRDVMGCEVLELWHKNTDLGGLTT
                     NDCVVLPGGFSYGDALRTGAIARFSPIMQAVTEHCNMGGFAWGICNGFQILCEAGLLP
                     GVLLENAHQQFTCKNVWLKAANHESMVNAAVGDNALCIPIAHAEGRYYADAETIQTLE
                     DNKQVLFRYCDANGVITDASNPNGSVNNIAGVCNAGRNVYGMMPHPERAAEGALGNTD
                     GYRLFASFLTSAGVPVA"
     gene            1511..3946
                     /locus_tag="PK742_00015"
     CDS             1511..3946
                     /locus_tag="PK742_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HQU74998.1"
                     /translation="MRRSITAALFFLLVLPVTLRAQLLQPVTWQMSAEKTGTNEYTLI
                     YKATIEEGWKVYATDIETGGPVPTSVNYEKIPDGLTMQGELQQLGRKEEAVEPLFDNM
                     TLKFFHKRVTLRQVVTITKDVTLKGFVEFMTCDDRQCLAPEAVDFEFKLVADEAGSDT
                     ETTGHDGILTPIKWKVYSEKIDETNYYIYFDASIDNGWKLYSQNIKPGGPVPTSFKFK
                     NAGSGYALIGKVDEVTRPEEKKEPLFDNMMLRYFKHHAQFRQHITLTDTAAVVRGSYT
                     FMTCDASQCLPAEEVDFAIDLRSGLIIATEEPGEDTLEVAEADFGINTTPLGSCNDET
                     GDTVTTAEGKNRGFAGIFLLGFVGGLLALLTPCVFPMIPLTVSFFTKRSQSKSKGVFE
                     ALFYGFSIILIYALLALPFVIYKLPPDTLNAISTNVYLNIAFFVVFLAFAFSFFGYYE
                     LTLPNSWLNKADSASNVGGLVGIFFMALTLALVSFSCTGPILGSLLVGSLSAEGGQMN
                     LMAGMIGFGAALALPFGLFAAFPQFMNKLPKSGGWLNSVKVVLGFLEVALAFKFFSNA
                     DLVAHFGILKREVFFIIWTLCGIGIVIYLLGWIKFPHDSPGKVKIGPARAAFGIIFLA
                     LSVHFGSGILKGGNPFLASGFAPPMFYSIYPQDTHCPLGLDCFHDFDEAVAYAKEVNK
                     PVMVDFTGWACVNCRKMEEHVWPEDEVYKLISEEYVLVSLYVDDKEMLPADEQYVSAF
                     SGKKIRTIGNKWSDFQSSYFGVNSQPYYVLISPDGQVLNNPVPYTPDVEEYASFLECG
                     LSSFREAEQVAEK"
     gene            complement(3948..5687)
                     /locus_tag="PK742_00020"
     CDS             complement(3948..5687)
                     /locus_tag="PK742_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQU74999.1"
                     /translation="MRIIIRIVLMLFPLTVSAQNWEYAIRNQVEIITLFLQDLDESFG
                     FLSEAEQTEYVNRYRENLYMHQSFCYSFADRFPELADQAMDLALATRGMILNSSIDMR
                     QTILSGGDTSAVRLLDEWTENKEKIAREYALPPDKRIRGLAALEKRNNLMEKDLYRMA
                     RSMRTTGDLLSVRWTDIRSALGEQETAVEYTNFRYYDGNRWTDSVIYVAIILRKDMEH
                     PQLIRIGTEKELQQLTGTVLNKSVESGMDELYGWPELDEDVAVYMGSHLLETFWKPLE
                     EWIPEGDTVFYTATGMMHQLNLDVIPIGSGRRLLDRNTFITCSSLSVVTQTPYGAGNI
                     HNIIAFGGMQYDLSEQEWNQATASFPQPGSFPGLSGSLKGSSTWTFLPGTFQEIQAIS
                     KLESAVVHVVAYSGAEAVEEQIKALGPQNPPDILHIATHGFFIPPDHGEELDVMDRSG
                     LLFSGANAAWSGKPAHGHEDGILTSREAANLFLPHTRLVVLSACETGLGEASGYEGVY
                     GLQRAFKAAGADYLLMTLWTVPDVQTAYFMQYFYQSIADGNGIETAFQTARTLVRKRY
                     TNPYYWAGFVLLH"
     gene            complement(5745..7559)
                     /locus_tag="PK742_00025"
     CDS             complement(5745..7559)
                     /locus_tag="PK742_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HQU75000.1"
                     /translation="MNKWTNLLFIPAVLLASCAGEKKEDNSAVQAAAQRYLDQYSQEY
                     QALYYASAEGQWTLNTRIVEGDTTASAAAARADEAYAAFTGSSANIDSCRKYLAMKDK
                     LLPLQVRQLEAMLFMAGNNPASAGEVVKERIDATNHQTELLYGYHYMLHGKEVSTNDI
                     DNLLRDEKNISRRLDAWNASKEVGTTLKDGLAELQRLRNASVTPLGYDDFFAYQVSEY
                     GMTSQEMMELTHQLIRDVWPLYRELHTWARYELAARYNEPVPDYLPAHWLPNRWGQDW
                     TAMVSVEGLDIDPALKEKGAEWVMEQGEAFWVSMGFPELPQTFWDKSSLYPLPEDADY
                     KKNNHASAWHMDLQDDVRSLMSVEANTEWWSTVLHELGHIYYYMSYSTPDVPMVLRGG
                     ANRGYHEAFGSMIGLASLQKPFLENLGLIEPGLQTNDTLLLLKEALDFIVHIPWGSGV
                     MTEFEYELYANNLPADQYNAKWWELVKKYQGIVPPSERGAQYCDAATKTHINDDAAQY
                     YDYSISNVLLFQFHDYIARNILHQDPHATNYWGSKETGEFLSGVMATGATVDWREHIR
                     KSIGADMSAKPMVDYFGPLMEYLQRVNAGRTCTLPESI"
     gene            7732..8730
                     /gene="recA"
                     /locus_tag="PK742_00030"
     CDS             7732..8730
                     /gene="recA"
                     /locus_tag="PK742_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQU75001.1"
                     /translation="MAEAADKLKALGLALDQLNKTYGKGTVMKLGESPEINVEVISSG
                     SIGLDTALGIGGFPRGRIVEIYGPESSGKTTLAIHAIAEVQKKGGIAAIIDAEHAFDK
                     TYAEKLGVDVENLLISQPDNGEQALEIAEHLIRSGALDIVVIDSVAALVPKGELEGEM
                     GESKMGLQARLMSQALRKLTGTINKTGCTCIFINQLREKIGVMFGNPETTTGGNALKF
                     YASIRLDIRRIGQIKEKDEVMGNRVRVKVVKNKVAPPFRMAEFDIMYGEGISKLGEIV
                     DIGVDAGILQKSGSWYSYDSTKLGQGREGVKELLRDNPELSDEIEGKIREKLKEGK"
     gene            complement(8797..9549)
                     /locus_tag="PK742_00035"
     CDS             complement(8797..9549)
                     /locus_tag="PK742_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75002.1"
                     /translation="MRKTLLAAAISIGLIGATTAEAANLCFSDSYGFTYQVTATKTGP
                     MYWEMTGVADVLSGYLWDVEGYWDRFNQSFYMNVINPEGCATYVDNFEFFSTFWTPGY
                     LEMSWNNYCDGYLGSGTNTMTYSGGSCGLRTSGETFQVGPNAVGASPFGLVELNITET
                     RSLTELLEEQEVTVNRMGEGFVFFGDVFTHDGTELIIYNHAGQQIAVLNGNGSNSIVW
                     DGIATAGNVAVNGMYVAVIRSEEEKLTVKFVK"
     gene            complement(9661..10428)
                     /locus_tag="PK742_00040"
     CDS             complement(9661..10428)
                     /locus_tag="PK742_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75003.1"
                     /translation="MKKKLLLAVLAIGMLSTSSFATIICFTDAFGSSYQINATKTAPK
                     YWTLSGEIGVGAAVPWPVDGYWDAFNQEFYITVSNPSPDGCTFYVDALNIWSTAWAPG
                     TMSWNYEQVCDGAVVFTGTPFDLTYTNGACVGRSMEPDYSNSIFTVGFDEAAAVHYPL
                     QVTETRTLDQILMEEEMSVNRMGDGFVFFSNVFTNSGAELIIYNHVGQQIAVLNGNGS
                     NTIVWDGIATSGQMTDPGMYVAVMRSENEDVTVKFVK"
     gene            complement(10539..11642)
                     /locus_tag="PK742_00045"
     CDS             complement(10539..11642)
                     /locus_tag="PK742_00045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQU75004.1"
                     /translation="MKRTSNRQAGQKKTRKVTKEKTQPLTKEEAKLKRERSQHERTLR
                     FSKKRKDEMSPEEREALASMTNRERKQAYYAKKKDKLTSPKKKKTEQEPLSFKGRSAQ
                     RAAPKRTLRKGQFIREQDIQKPKKGPDEGMRLNKFLANAGVAARRKADELIKAGLIKV
                     NGEVVMEMGYKLHPDDEVTYKGKKVKPARFVYLLLNKPKDYLTTVTDDRDRRTVMDLV
                     AKATQERVYPVGRLDRHTTGLLLITNDGDLAMDLAHPSRGVQKVYEVTLDKAVEKHDM
                     EAIAEGLELEDGKAMVDEIAWPDPHRKNVVGISLHIGKNRIVRRIFEHLGYQVTKLDR
                     TVYAGLTKKDLPRGRWRYLTPREVVQLKHLGKK"
     gene            complement(11539..12288)
                     /gene="scpB"
                     /locus_tag="PK742_00050"
     CDS             complement(11539..12288)
                     /gene="scpB"
                     /locus_tag="PK742_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQU75005.1"
                     /translation="MDKLQQILEALIFSSEQPISVAELQTVLFTYSGEEVSLEAIQEL
                     VDQLKTKYDSDDYVFEILQSGGGYQFLSKPLYHKPISTLLQHRAKRKLSTAALECLSI
                     IAYSQPVTKTDIEQIRGVNCDYTLQKLLERDLIRITGRADTPGKPLQYGTTQFFMDYF
                     GINALDELPKLKEFEQKDLSIGDPGDIIVSAEENDDHTITLKVSAERREDAQNQQHEE
                     NIEQTGGSEEDPEGDQRENATPDEGGGETEA"
     gene            complement(12529..13410)
                     /gene="atpG"
                     /locus_tag="PK742_00055"
     CDS             complement(12529..13410)
                     /gene="atpG"
                     /locus_tag="PK742_00055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQU75006.1"
                     /translation="MSGKLKEVRTRITSVTSTQQITKAMKMVSAAKLRRAQDAILQMR
                     PYAIKMGSMLENIAAATQGDVRIEYADQREVKKALIVIITSDKGLCGAFNANVAKAAR
                     RVIEERYSHLPATAVNLMFIGKKGYDVYKRSNYTLLTKYQNLFHNLNFEDVKAATEYI
                     KTGFLHKEFDEVMVIYNKFKNAATQILETEQFLPIRKVDEGETQAKTDFIFDPDKHEL
                     IGELVPRILNTTFYRFILDSHASEHGARMTAMDKASDNANELLKDLRIAYNRARQAAI
                     TTELTEIVSGAAALQGN"
     gene            complement(13430..15010)
                     /gene="atpA"
                     /locus_tag="PK742_00060"
     CDS             complement(13430..15010)
                     /gene="atpA"
                     /locus_tag="PK742_00060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQU75007.1"
                     /translation="MVDVKPDEISAILRQQLSNFNNASELEEVGTVLQVGDGIARIYG
                     LNNAKSGELVAFENGVRAIVLNLEEDNVGAVLMGASDQIKEGDTVRRTGRIASINVGD
                     GLVGRVVNTLGEPIDGKGPIEGDLFEMPLERKAPGVIYRQPVNEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIALDTIINQREFFEAGNPVYCIYVACGQKNSTVAQVAKT
                     LQDHGAMDYTIIVSASASDPAPLQFYAPFAGAAMGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVINNDEIAANMNDLPESIKHKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNAGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATMNVLNKGKRNVEILKQKQY
                     APITVEKQIAIIYLGTQGLLGNVPIDKIKEFETTYLAFLETHHKEVLDQLKAGQLTDE
                     VRAKLEEVGKDMAKKYTA"
     gene            complement(15032..15592)
                     /gene="atpH"
                     /locus_tag="PK742_00065"
     CDS             complement(15032..15592)
                     /gene="atpH"
                     /locus_tag="PK742_00065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HQU75008.1"
                     /translation="MSAIRIANRYAKSILQLAIEKGQLEEVNRDMEMLNVATDDSRDF
                     YLMLKSPIIHADKKMAVINGVFGNKLTSITSNFLEILVRKGREGFLPDVIDVFRNMYN
                     AHKGITEVELRTAYELSEQTRQAIMDRLRSMAGLQEIRMKTTIDDSLIGGYVLRFEDK
                     LIDTSIQRGLNILKDDFDNNDYIRKF"
     gene            complement(15605..16120)
                     /gene="atpF"
                     /locus_tag="PK742_00070"
     CDS             complement(15605..16120)
                     /gene="atpF"
                     /locus_tag="PK742_00070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQU75009.1"
                     /translation="MFILIDALDLVTPDPGLFIWTVFIFLVVFFILWKFAFGPISKAL
                     GDREKGIEEALQSAVRAKEEMANMKADNERILNEAREERSKMLREAKEAKEQIIAEAR
                     EKANTEYNRIVEEAQQQIDNQRMAALIDIKNQVGNMVLEVSEKVLRRELQDRNAQEAY
                     TRSLVDEIKVN"
     gene            complement(16198..16434)
                     /gene="atpE"
                     /locus_tag="PK742_00075"
     CDS             complement(16198..16434)
                     /gene="atpE"
                     /locus_tag="PK742_00075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQU75010.1"
                     /translation="MELLTVLQEAGINGIAAIGAGLAAVGAGIGVGRIGGSALESIAR
                     QPEAIGDIRSNMIIAAALVEGVALFAVVVALLQG"
     gene            complement(16470..17615)
                     /gene="atpB"
                     /locus_tag="PK742_00080"
     CDS             complement(16470..17615)
                     /gene="atpB"
                     /locus_tag="PK742_00080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQU75011.1"
                     /translation="MKKIIQVVLSAILLLSAGSLMAAEEGAYNPKDFALHHIADANTW
                     HIVGNVSIPLPVIVYHKEDGLAIFLSNKLPEAHHAEEVETEMAHEEHGMDMGKVEEGM
                     EQEHPDMEAAHHEPASYKGYVMVHDRVARADGTAFLDLSVTKNVATLILTALLLIWIF
                     TSVARAYARRPGQAPKGLQSLMEPLIIFVRDDVVKPNLGDNTERFLPYMLTLFFFIWF
                     ANMLGLIPLISNPNLTGNITVTMALSVLTFILTNVNGTKDYWMHILWPPGVPLWVKII
                     LVPIEILGIFIKPFALMIRLFANITAGHIIILSLVGLIFIFGNAGESLGGGLGGALVA
                     VPFTIFMSFLELLVAFLQAFIFVMLSALFISLAMEKHEHHHEEAVHH"
     gene            17708..18448
                     /locus_tag="PK742_00085"
     CDS             17708..18448
                     /locus_tag="PK742_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQU75012.1"
                     /translation="MKVKFYAVSGALTALLLILSCGNKAGRDYQAENTVPAIKHPSVT
                     GDLENQFLEAGLVDIAGVVPGIQVDLKYSTTDNFMHTDMYGSLEKAYLHPEVAAKLAK
                     AAGALSDSHPDLHFLVYDGARPLSIQQFMWDHVDLPPDQRRQFLSPPDQLSLHNYGAA
                     VDITLATADGLAIDMGTPYDDPGVLSYPIRESEFLANGSLSAEQVANRQLLRSVMREA
                     GFTGIDSEWWHFNSCDRQFAAAHYTLLR"
     gene            complement(18449..19834)
                     /locus_tag="PK742_00090"
     CDS             complement(18449..19834)
                     /locus_tag="PK742_00090"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQU75013.1"
                     /translation="MMKASLLTLFACLISSLSWCQTDGMLFNYMGMGASTALVRDYQS
                     IGINPANLGYDTDYKLSFEIGSMGLSVYSEALTKEEVRYFQPTGGEQLTQEQQAAIAE
                     TLLQDGFQTDVFVRPVAFGFDLGNAGGLAIGLSVTANQSFTLGSEASNLLFQGFNYEG
                     YIDTIIIEDTSNIYGVAYEPLSLAGLTEGTSLHFNLRSAASIAWGRRWFASDHLNIYA
                     GIGLNYIMSYGWLDFRSQNEEIVGQAALGLDLVNSPDYIETFDPNQQPLQPVGTGFGF
                     DIGFSADLGEDLSVGLSVVQIGKVSYDLNTLVFNNIIVDTIYFNGINSTQTLDLVSEL
                     LDGEQLLDYNGIERFEVPLPTALRAGAALKMSDMLTLAADISLPLSSTAQDFSSVQAG
                     AGARLTLLRFLQLSGGLTAGGNYGFNVPAGIGVDLGFWEFGVSTRDILVFLGQSKPTV
                     SYAIGVFRFKI"
     gene            19867..20571
                     /locus_tag="PK742_00095"
     CDS             19867..20571
                     /locus_tag="PK742_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75014.1"
                     /translation="MQSNFRLTGSLFSVLIVLLCMPLATSAQKSIAVSEVQETMSMGT
                     RNGFQAILSDQSKKDVEAVLEDLLKEYDKKAKMEKSGKNEAKVDDVIIPSISDQPVDL
                     YFLFSEDKSSCTVTGFFDMGTSFLSSQVYPDRFRSGEVFMQKLGLRAEKTRLAGLVKA
                     ATDDMQSLERDKAGLEKDKEKLEKSIADCEATIAKAKSDLDTNASDTKKKSEEIGQQK
                     EKLGSLQKELDQYRDH"
     gene            complement(20565..21272)
                     /locus_tag="PK742_00100"
     CDS             complement(20565..21272)
                     /locus_tag="PK742_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU75015.1"
                     /translation="MEEPKIRILLCEDDPNFGAVLKDYLTMNGYEVTLATDGDKGVDI
                     FRTEQFGLCVLDVMMPKKDGFTAAAEIKRMNPDIPVIFLTAKTQQQDKIEGFKIGADD
                     YITKPFNSEELLYRIAAVLKRGRLKQNNQQKEFEVGKYHFNYKNRILTHDGVQEKLSP
                     KEAELLRMLCMKVNDILYREEALKQIWGRDDYFTARSMDVFITKLRKYLKDDPNLEIV
                     NIHGNGFRLNVEEATAQ"
     gene            complement(21278..22870)
                     /locus_tag="PK742_00105"
     CDS             complement(21278..22870)
                     /locus_tag="PK742_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQU75016.1"
                     /translation="MKQARIRIIIALMSTALLGIILVQVYWIQSTLNQKEKIFGYQVA
                     DALTRVSENVETHWIATMLKSHTDLIFSDSAFAVNGSMRINKTWVSDTLIQEAMQSEH
                     INIAALTEPGASYSYPAPGALPNRPTMILEPVEMGTRVGQEGGNDEIISTILKEIDKQ
                     FQVSADKLDQVMQQMIVEMIMQGAQPGDKVDTAYLSQMLQLEFRNRGIETEFQYGVLL
                     NGKTLISPVADEQKSTLLKTQHTASLFPNNIFFGKDLLMVQFPDQRTYLLQSIWVLLL
                     GSLLFTSVIIFVFSYSIHIILRQKKLSDIKNDFINNMTHEFKTPLATISLAVDAINSP
                     LVLQDEQRVKHYTGIIREENRRMNGQVEKVLQAALLDKHELNLKPEELDLHEVIEHAV
                     DNVTLLVEEKQGRITKRLEADNALFTGDNVHLVNVIYNLLDNASKYSPETPDIVISTR
                     NKGNHILISVQDHGMGMSADQQKMIFEKFYRVPTGNVHNIKGFGLGLTYVKTVVEAHG
                     GIIEVKSAPGKGSTFTIQLPQN"
     gene            23039..24547
                     /locus_tag="PK742_00110"
     CDS             23039..24547
                     /locus_tag="PK742_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQU75017.1"
                     /translation="MKAITKWTSGTVMMLAVLIQLPASAQENDQIRLKIDANVNGQHI
                     QIDTTIEQMEDFDLQQYLRELGLEEEMDGLQTMDIRIRDNSDHTFFNFDELENAEWAN
                     DLKLHLESIDMPEMPELPAMGHVMFFNGNKAFLGVVTDNTKSRDGVLVNEVVENSAAA
                     AAGLQRGDIITMINDKTVESTNNLIEILSTYQPGEVINVTYLRDDSKQQATATLKENE
                     GYFESAEWEQYGERWEEWGEAFEKKMENLGDDFQMRWESNMADVQKPFLGVYLNEHDQ
                     GAEITGVSEGSAAEAAGLQKGDIITAIDGITMETTRQVSDYIRSKQIHDEVSVTVLRQ
                     GTSIQIPAILGKHNSDLLFWMDDEKGVSPLGNEERHIFFSPGNGNCHSYSYELNDDGK
                     KTIIITMDLENEQSSSTDSARTGNVFRAEDVEFYPNPSNGTFNLRFAVEEPSDVLVQI
                     RDARGNMVYEENLENYSGTYDHTISLGNEAKGNYFINIVRGDYIITKQIVIQ"
     gene            complement(24594..25538)
                     /locus_tag="PK742_00115"
     CDS             complement(24594..25538)
                     /locus_tag="PK742_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQU75018.1"
                     /translation="MMNRFDHIESVYWVFTQLCNDQCDHCYNLSGPRGARLQDEEVLQ
                     IIDNLPQKMDRLILSGGEPLAERKQLFLLLDRVAERYGSSTQIMLQTNGDLMTGQILD
                     TLLEKGVSRIDIASIDRYHKHAGKRIDQLKELFLSRGMTDDNTSPLIEKDTYLHKQSA
                     SFGFWGATEDMWLGGNWARGRAMEKDIWKKDPEHNFCNILSGAIGFLGGVDDIPQEIS
                     IQLWKINPCCPGTRFPLGDARTEKVADVLERIASNPVFQRLNEGDPWKMGESFGISEA
                     YAHERTLALKNVCLWCDEFFETYLNPADFIPLQVIQPA"
     gene            complement(25535..26254)
                     /locus_tag="PK742_00120"
     CDS             complement(25535..26254)
                     /locus_tag="PK742_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU75019.1"
                     /translation="MELKHISKSFGAQQVLTDISLVLEEGHTLAVLGKSGCGKTTLLR
                     IIAGLELADNGEIWLNGKNMSGVAVHKRNAVYLNQEPLLFPHMTASANIAYGLEVRGV
                     PASDIRRQTEQMLAELGLEDQGHKRPHQLSGGQRQRVSFGRAMIIHPSVLLLDEPFGN
                     LDPDTRKQMQELFMRLSTAHRITSLLVTHDLKEAILTGQQTAFMESGVLTMYPDTGAF
                     LSDPRTGGQDEMAFWKKYNQS"
     gene            complement(26254..27030)
                     /locus_tag="PK742_00125"
     CDS             complement(26254..27030)
                     /locus_tag="PK742_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQU75020.1"
                     /translation="MHRRKTYASLVVSIILAALPYGILPLMSFFGRWHFPALLPETWT
                     FQHWGSFLHSQGAVGASFLLSLGIATCTATLASTGGLLLSRAVSYSSFRQGGLFFSLL
                     PVTMSPVLFATILLFYFQVAGLAGTVAGVILAQMLIALPFAIIYFHSFWSVRILRLEQ
                     LSATLGSTPVHTYRYIIWPVARPFLLVCFFQTFIISWLEYGLTSVIGTGQIQTLTLKV
                     FLYIQESNPSMAALSATLLILPPAILLFWNRRFVFKEVSL"
     gene            complement(27023..27865)
                     /locus_tag="PK742_00130"
     CDS             complement(27023..27865)
                     /locus_tag="PK742_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75021.1"
                     /translation="MKAGKSIAWIVFVLLVILPVSASLVYAFLYSVGLTGLLSDGYTI
                     AYWQQLRTNSPFFFSLLYSMIIAVISIGLAVCIALWLTLRYGTTLQRRNISFILLLPL
                     GIPAVVAALMTYQLLSPSGWLSRLTYAVGLTAGPDAFPALINDRLAVGIIITHIILAV
                     PYFTALFRNIYRQEQGDMLLLTARNLGAENGQASRTIVIPLLLRSAFPSILMFLLFVM
                     GSYEIPLLLGVSDPRMLSPLIADKMFRFDLLDKPLAYAMLVSYVLLVLLLNMLLLRKN
                     ARHA"
     gene            complement(27852..29057)
                     /locus_tag="PK742_00135"
     CDS             complement(27852..29057)
                     /locus_tag="PK742_00135"
                     /inference="COORDINATES: protein motif:HMM:NF008633.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQU75022.1"
                     /translation="MKYFSILFIALLFSSCHRKGDQATVPGSWDEVVAAGTGSTVRMM
                     MWQGDPFINTYMQQYVKPKLKELYGIELEISSGQGNMIVSLLLSELEAGKKQSSIDMM
                     WINGETFYQLRQIDALYGPFSGILPNERYIDWDNPYIATDFQQPINGMECPWGNVQLS
                     IIYDSLRTPEPPRTMTELLTYVQAHPGSFTIPNEFTGMTLLKSWLIAIAGGDSVLAGP
                     FDEQKYQHYSAQLWEYIRALQPYMWKEGKTFPDGPATMHRMLANGEIDFSMSNNDGEV
                     DNKVLQGILPETARSYVFTSGTIRNSHYLGLTQRSSNLPGAMMVINFLISPEAQFEKM
                     QPAVWGDGTVLRTDELPDNWPQQFREIPGRKYAPDRASISRYALMELSPEYMIRLFDD
                     FRNIIIEGR"
     gene            complement(29102..32098)
                     /locus_tag="PK742_00140"
     CDS             complement(29102..32098)
                     /locus_tag="PK742_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU75023.1"
                     /translation="MRTFALTLLHILFVSTIIAQVDLEVNVTDLASAGPLEGITVSIQ
                     NTAIGYTDQSVTDAQGKVRFRSLPVNGTYTISAAGNASYSGVSLSEVELKNNEKASVQ
                     LAMPRKTETTLDEVAITAASTSRINTINAEVSSELSLKEIRQIPVEGRDITRVLFRLP
                     GVVQATGFYPEAPNVSIMGGNSLYTNYTIDGFDNNERFLGGQKFAIPVGFARNISVLT
                     NNFSTEYGLTGNGIIDITTRSGSNDFSAEAFYLTRPGAVIDTLGVGASPYTQRDLSGN
                     QVNNTFMRQQLGIGFGGAIKPDRTFYYVNAEQTFDLKENVLDVPQLGLTEAVPGRNNF
                     TYLSGKIDQVWNKRFRSSLRANVGLVGIERQGGGLEGGVVFPSAANTQMRNSFLLASK
                     NSYVGDGFSTETNLQFSRFRWNYADPVNGAGPNTNVIGPDGLTVAILGHPGYYFDDLE
                     NTLQFQQKLNIYRENHTFKTGVEVISSDFQLAGGGNPDGSYTVQVTEDQLAQLATFGS
                     ALDVNDIPSDVQVLGYAVELRPATFGTRQNIFSVYAEDIWSVSNKLNLTYGIRYDYDN
                     LSKGGGDQGDFNNIAPRASFNYKLNSRSVIRGGYAMVYDKILYAIYSDALQQNTTSDD
                     YRAQLQALIDQGILPSTTDLDKITFEGNLSAFASGVPYLNGPSADDLQSQRDQAFSNE
                     RRILNPNGYQNPQTHQISLGYQVQLTEDKLFYIDGFYNKTNFLYRLVDLNAPEAYPVD
                     ENFEAGDVRDQATADLTRPVAIYEGSYAIINGDTVYGVARNVVMSETAGEARYYGISL
                     NLQKERMNDNYAWRFIYTLSRNENNTEDINFRAMDSNNFEDEWGPSINDRTHVFNTIF
                     NLYPCDGWEFTFAGLVQSGMPINRIPDASVYGTTDLNGDGRSFGDAYVGNSDRSPGES
                     RNNDRLPWSNTIDLGAQYTWSLGTGNLEFRADVFNLLNAQNLSGYSNNATQSNQVQVG
                     PADSGVLVRRNAAPPRQFQFGARYYF"
     gene            complement(32140..32820)
                     /locus_tag="PK742_00145"
     CDS             complement(32140..32820)
                     /locus_tag="PK742_00145"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HQU75024.1"
                     /translation="MDRSCTDTAILVFARHPEDEARHKNWLGARRHRANTTLASRLFR
                     SICSEAHATGLPVFVIDSTRQQGQSFGERFHHAITTVFAAGFDKVITVGSDCPFLRSH
                     HLLQAASRLDKGLVLGPDHRGGVYLMAIDRKTFDEADLLNIHWQTPVVLKELMQLATV
                     NLLTRLHDLHSLQDIQRLLARIRKPSGLQWLLSFLIQASDQKNLPELPHTRYMNRVHI
                     RRGPPVLA"
     gene            complement(32890..34206)
                     /locus_tag="PK742_00150"
     CDS             complement(32890..34206)
                     /locus_tag="PK742_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU75025.1"
                     /translation="MSKKVLFLFFPIVLFFSCSKETISPEIFELTALQDAVVYEVNFK
                     AYSPTRNLQAVIDRLDDLDSLEVNVIWLMPIYPEGQVNAVGSPYAVADYTEVNPDLGD
                     LNKLTELVGAAHAHGMAVILDWVANHTAWDNPWITEHPDWYTQDGSGNIISPPGTGWN
                     DVADLNYSNNNMRLAMIDAMWYWITETGIDGFRCDAADLVPFDFWQQANDSLRSRANK
                     TLFMLAEGARPDHLTAGFDLNFGWSWYGDLKNVMNSNADVGSLPVASSTAPLHNRLVF
                     TTNHDESAWDAAPPTLFGSQEAALTAFALTLTSGAGMPLIYGTQEVGRTGTVSFFNQS
                     TTNWLSNPEILQAYQDLMRLYRIHPALRRGNREWFDTGYHMILFTRSWLNDQLLVMIN
                     PRDVEISTVCPETLRGVWVDLLTGDQLVLQDAFSAAPYSYRIMIRN"
     gene            complement(34231..34719)
                     /locus_tag="PK742_00155"
     CDS             complement(34231..34719)
                     /locus_tag="PK742_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75026.1"
                     /translation="MKVNHLLMAMTLGLTLSACSANKQVEKTTDHFIQALEQQNWDEA
                     KALTTPESHGFLAWLESANFGSSEDGDVEMYQYQILSDKTVINGDSATVTTLDEFNNE
                     SVYRLVKVDGKWLVDFTIPGMGDMEQQTQELNDLQETLDDMGDAIDDLSEQLEDLGNE
                     AR"
     gene            complement(34762..35196)
                     /locus_tag="PK742_00160"
     CDS             complement(34762..35196)
                     /locus_tag="PK742_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75027.1"
                     /translation="MVNLKVRYPLLLFMMLVLGSCSRPDYGALLHFNNAELYFTERVT
                     ESEATRLGEYLVDYGIFSGQVITVQLDKDQTTYLFRWVFPDTTTLDSTALRNVGLFTI
                     QLSEEVFHHAPVDIHFCNERLETQKVVPFLATNTEAVRQDQE"
     gene            complement(35269..38541)
                     /locus_tag="PK742_00165"
     CDS             complement(35269..38541)
                     /locus_tag="PK742_00165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HQU75028.1"
                     /translation="MAAHKAKSNGLSFQRNFTRDDISPFDMFEYDNRSSVIRNTTGDI
                     VFEMNHVEVPNSWSQVATDILAQKYFRKAGVPQKDGSTGSETSIKQVAHRLADCWKQW
                     GEKYGYFASSKDAQIFYDELVYMIIGQYAAPNSPQWFNTGLFTAYGISGKPQGHYYVD
                     PDSGKLKKSTSAYERPQPHACFILSVDDDLVNPGGIMDLWTREARIFKYGSGVGTNYS
                     RIRGEGERLSGGGTSSGLMSFLKIGDAAAGAIKSGGTTRRAAKMVCLDLDHPEIENFI
                     DWKVKEEDKVAALIAAGYPSDYEGEAYRTVAGQNSNNSIRIPNSFFKALEEDGDWELK
                     ARSNGETMKKVNAQKLWDKIAHAAWRCADPGVQFDSTINEWHTCPADGRINASNPCSE
                     YMFLDNTACNLASINLRKFYDDEKLVFDVAAFENACRVWTVVLEISVLMAQFPSEEVA
                     RLSYEFRTLGLGFANLGSLLMVSGIAYDSDKARAIAGSISAIMTGISYKTSAEMAAAI
                     GPFAGYARNKEHMLRVMRNHRYAAYNAADNFEGLEIKPMGIDQKLCPEYLLRAACNAW
                     DDAVRLGEEHGYRNAQATVIAPTGTIGLVMDCDTTGVEPDFALVKFKKLSGGGYFKII
                     NQSIPLALRNLGYKEDSIKAIVDYAKGTGTLQGAPHINFESLAAKGFTDSELHHLDKV
                     VQSAFEITFAFNVFTVGEVALQRMGFKSEQYMDPMFNLLKALGFSQAQIDEANDVICG
                     TMTIEGAPQLQEKHYEVFDCANKNGKIGKRFIPATGHIRMMGAVQPFISGAISKTINL
                     PHEAVVEEIQECYRLSWELGLKANALYRDGSKLSQPLSTKSDKKDEVSTDHQWTEDEV
                     LEAARSLIMSSKDTMFKRQLSRVVERKKLPAKRSGFTQKAKIGGQTVFVRTGEYDDNT
                     LGEIFIDLHKEGASFRSLVNCFAIAVSIGLQYGVPLEEFADKFTFTRFEPSGPVDHPN
                     IKFSTSIVDYVFRLLAFEYLDRTDLVHVAPEEIEEKSPLDLSSEARGEYPVEKPHAKK
                     VEPVAVVVEKKATGANGDPVSAANKTLMGDAPPCHKCGHTTIRNGTCYKCLNCGESLG
                     CS"
     gene            38873..39634
                     /locus_tag="PK742_00170"
     CDS             38873..39634
                     /locus_tag="PK742_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HQU75029.1"
                     /translation="MHSIRSYIEDARSRHQKLFAVLVDPDDVDESSLDRIARQALDNG
                     VDLFLVGGSLVTGTKLPDTIARLKTTTNLPVVIFPGSVQQVDPQADALLFLSLISGRN
                     PDLLIGNQVLAAPYVKRSGIEAIATGYMLVDGGAPTTVSYISNTLPIPADKNDIAVCT
                     ALAGEMLGMHTIYMDAGSGARQAIPASMIRAVSTQVDLPVIVGGGIRTPDQAYLSAEA
                     GATVIVVGNAIEEDSNLISELSFAVHSASGQGVKR"
     gene            39651..40574
                     /gene="rsgA"
                     /locus_tag="PK742_00175"
     CDS             39651..40574
                     /gene="rsgA"
                     /locus_tag="PK742_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQU75030.1"
                     /translation="MRGRVIQSTGSWYEVLLDNGASISCRLKGRFRLEDKKLTNPVAV
                     GDVVEVDADQEGSSVITEIGKRQNYLVRESPQKKEHTHIIASNIDQVVVIATLHQPRT
                     STGFIDRVLFMAEVYGIPGYVVFNKCDLYDEEEMDQLMEYGGMYLEAGYPAFHTSATT
                     GEHLAKLKSLLQNKTTLLTGHSGVGKSSLINALQPELNVRVGDISAYHEKGQHTTTFA
                     RMFPLAFGGFIVDTPGIKEFGMVHVTAEEAGHYFPEIRERMEHCKFNNCVHYNEKECA
                     IREAVETGHMSGRRYLSYLSIYEELQAKKPW"
     gene            40575..41027
                     /gene="dtd"
                     /locus_tag="PK742_00180"
     CDS             40575..41027
                     /gene="dtd"
                     /locus_tag="PK742_00180"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQU75031.1"
                     /translation="MRAVIQRVSKASVTVDGEVISRIGTGLLVLLGVEDADTPEDADW
                     LVRKIIQLRIFADEQGLMNKSIQDVGGDLLVVSQFTLFASTKKGNRPSFIRSARPDHA
                     IPLYEEFVQLAESLLGRQVATGSFGAMMEVDLVNSGPVTITIDTKNKE"
     gene            41024..41647
                     /locus_tag="PK742_00185"
     CDS             41024..41647
                     /locus_tag="PK742_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75032.1"
                     /translation="MKRIKAFFQRYTEIILVILTIVFVFYKSSLPKYGERISLMFFGT
                     LAFYYLASGVLVFLDRHRILRTIRLIYLIGLWAVSMAVIGIMARIHLIRMNEELLYIS
                     CAALAGTLAFSWLAYRQVDEGEQQQAWRWQLQPIVIRSTAALLFSLGIVLITPYTVYH
                     TFGTYRNDAQYTEDAVYVYEHPEDTAAQRKLEEATLLKSEDGKRNAE"
     gene            complement(41715..42188)
                     /locus_tag="PK742_00190"
     CDS             complement(41715..42188)
                     /locus_tag="PK742_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75033.1"
                     /translation="MRFLFSFFCVCLSCHTFAQSELHFDDGIILRVEIDEFNYLDHYY
                     DTCSPNETPYICRIDGEEWFGMDRGMELPKYQLKSLIFIDEDDTISLDVSRMYNPTFY
                     DGISNKHFLLEKSDDILEIFGWFSDGAGTYCAKWIISNSVAHRILLSNSEDDCFN"
     gene            complement(42280..43017)
                     /locus_tag="PK742_00195"
     CDS             complement(42280..43017)
                     /locus_tag="PK742_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HQU75034.1"
                     /translation="MSIIKRPVDSDPVIEVCLTPALWYQFDYAEKNVVVIDIIRATST
                     ITAALYHGVEKMIPVETVQEAEAYLSKGYLVAGERDGYKIEHFQFGNSPYEFMGDDVK
                     GKTIIHTTTNGTRCVLMSKGARNIVAGSFLNIEAVVDWIREENRDTILFCAGWKDHYN
                     MEDALYAGSVVRALSDQFQILDDATLSTKMMYRYIRANMLRSVKQAQHFKRLAKLGIE
                     KDIKFCMGNHKYDVVPVYDGEGFVRMK"
     gene            complement(43024..44112)
                     /gene="gcvT"
                     /locus_tag="PK742_00200"
     CDS             complement(43024..44112)
                     /gene="gcvT"
                     /locus_tag="PK742_00200"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQU75035.1"
                     /translation="MKSTALTHIHEQLGAKMAEFAGYNMPISYSGINDEHQTVRNAVG
                     VFDVSHMGEFILKGEGAADLVQRVTSNDVYALSPGKAQYSCLPNNDGGIVDDLLVYHL
                     EDGNYMLVVNASNMEKDWNWISQHNTGGVEMHNISDQTSLLAVQGPKAVEALQSLTDV
                     NLSEIAYYTFTKHRFAGVDNVIISATGYTGAGGFELYFPKEHSEVIWNAVFEAGAAFG
                     IKPIGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKLKAANNEFTNKAHFVQQ
                     RADGLKKKLVGFEMVDRGIPRHDYPIVDEAGNVIGKVTSGTQSPTLGKAIGMGYVDTA
                     FAEPGSTVYIDIRQKPVQAVVVKLPFLQ"
     gene            44194..>44878
                     /locus_tag="PK742_00205"
     CDS             44194..>44878
                     /locus_tag="PK742_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HQU75036.1"
                     /translation="MRFTESYTLADIAQWLGVSYAGSGDAPVTGLNEIHKVEAGDITF
                     VDFHKYYDAALNSAATFIIINKEVDCPAGKGLLFSDDPFRDFVKLAKRFRPAEHSLHP
                     VADSAVVGEGTILYPGVYVGPHVQIGKNCIIHPNVVLYDHTIIGNNVIIHAGAVLGAD
                     AFYYKKRKEPQPFFDKLYSCGRVVVEDNVEIGAACTIDKGVSGDTVIGKGSKLDNHIH
                     IGHGVVLGER"
BASE COUNT        11057 a        11131 c        10996 g        11694 t
ORIGIN      
        1 ggtgcctgat gcattcatcc atccggtttc ggtcataact gttataggct ttcttcgcct
       61 gtaccaattg atcttcaaca gttacaacaa gcaacaggca gacagtttat tgctcaatgc
      121 cggtatttgg tgcggcatcc tcagcttact tgagcctgcc tatattctta ttgttgtacc
      181 tgtggtcatt ggattttcta ctatcaggac ccttacattc cgcgaatcac tggtcatact
      241 ttcaggaaca ttggtcatgc acttccttgc agttacagct atgttattat tcggaaaatc
      301 agactggtat ttggccgcct ggaagaactg gcatataccc ggacaagatg gctggccaca
      361 ctggctgata ttgatccagc tggctctggc tcttatgttc ctgctggcag caactgctta
      421 cctgacccaa cacctgaaca atcagctgat ccgcttcaga agaaacacaa gggtgctttt
      481 ggtatttctg ctgggtgcct tgcttctggc attgatacta cctgccgggg taactgctgt
      541 ttggtggtgg atagccgcac ctgcagcact gatgatcagc tggtggtgga cacagatgaa
      601 gaatcaggat gctgcggcta tcctgcatct gaccttactc ggactggttt tcgtttttca
      661 atacattaac tttgcctgat aatcagagct tatgcgtttc ggtgtggtca tttttccggg
      721 ttccaactgt gatcaggaca tcattcatgt gttacgtgat gtaatgggct gtgaagtgct
      781 ggaactctgg cataagaaca ctgatctggg gggtcttacc accaatgact gtgtggtgct
      841 gccgggtgga ttttcatatg gagatgcact gcgcaccggt gccattgcac gcttttctcc
      901 cattatgcag gctgtgaccg aacactgtaa catgggcggc tttgcctggg gtatctgcaa
      961 cggattccag atcttgtgtg aggcaggact gctgcccgga gtgctgcttg aaaacgctca
     1021 ccagcaattt acatgcaaaa acgtctggct gaaggcggct aatcacgaaa gcatggtcaa
     1081 tgctgcggta ggtgacaatg ccttgtgcat cccgatagct cacgctgaag gccgctacta
     1141 tgctgatgcc gaaaccatac aaacactgga agacaataaa caggttctgt tcaggtactg
     1201 cgatgccaac ggtgttatta ccgatgcctc caacccgaac ggatctgtca ataatattgc
     1261 aggagtttgt aatgcggggc gcaatgtgta cggcatgatg ccccacccgg aaagagctgc
     1321 agaaggagcc ctgggcaata ccgacggcta ccgtttgttc gcttcttttc tgacatctgc
     1381 cggtgtaccc gtagcctgaa ggaaccgtca ggtattcccg cttgttgtaa aacctttgta
     1441 agcctttttc ggggctgatg acttaacttt accaaccccc tatttttgta ggttagatga
     1501 atttccggat atgagaagat ctattactgc tgccctgttt ttcctgctgg tgctgcccgt
     1561 aaccctccgg gcgcagctgc tgcagcctgt cacctggcag atgtctgcag aaaaaacagg
     1621 taccaatgaa tataccctga tctacaaagc caccattgaa gaaggctgga aggtatatgc
     1681 cactgatatt gaaaccggag gccccgtacc tacttctgta aactatgaaa agatacctga
     1741 tggtcttacc atgcagggag aactgcagca gttaggacga aaagaagaag ctgttgagcc
     1801 cctgttcgat aacatgaccc tgaagttctt tcataagcgg gtcactttac gacaggtagt
     1861 gaccattacc aaagatgtga ccctgaaagg atttgtggaa ttcatgacct gcgatgacag
     1921 gcaatgcctg gcccccgaag cagtagattt tgaattcaaa ctggtagccg atgaagcagg
     1981 cagtgacaca gagaccaccg gacacgatgg tatcctgacc cctatcaagt ggaaggtgta
     2041 cagtgaaaaa atagatgaaa ctaattacta tatctacttc gatgcatcca tagacaatgg
     2101 ctggaaactg tattcacaaa acatcaaacc cggcgggccc gtaccaacct ctttcaaatt
     2161 caaaaatgca ggcagtgggt atgcactcat cggaaaagtg gatgaggtaa ccagaccgga
     2221 agagaagaaa gaaccgctgt ttgataatat gatgctgcgc tacttcaaac accacgctca
     2281 gttcaggcag cacatcaccc tgacagatac agctgcagtt gtaagaggca gttatacctt
     2341 tatgacctgc gatgcttctc agtgtctccc tgcagaagaa gtggactttg ctattgacct
     2401 tcgaagcgga ctcatcatag ctacagaaga gccgggtgag gatacgctgg aagtagcaga
     2461 agcagatttc ggcatcaata ccacacctct tggttcctgc aatgatgaaa caggcgatac
     2521 tgttacaacc gccgaaggca agaatcgcgg ttttgcaggc atcttccttc ttggatttgt
     2581 tggtgggctg ctggctttgc tcaccccctg tgtatttcct atgataccgc taaccgtcag
     2641 cttctttacc aagcgctcac agagtaaatc caaaggtgtg ttcgaagccc tgttctacgg
     2701 cttcagtatc atcctgatat acgccctgct agcattgcca tttgtcatat ataaattacc
     2761 gcccgataca ctgaatgcca tctctaccaa cgtgtatctg aacatcgctt tttttgtagt
     2821 gttcctggca tttgccttct catttttcgg ctattatgaa ctgacccttc caaactcctg
     2881 gttgaacaag gcagacagcg ccagcaatgt aggtggtctc gtggggatct tctttatggc
     2941 actcacactt gcactggtct cctttagttg tactggtcct atcctgggca gcctgctggt
     3001 aggcagtttg tctgccgaag gtggtcagat gaatctcatg gcaggtatga tcggatttgg
     3061 agctgctctg gctttgccat tcggactgtt tgcggccttc ccacagttca tgaacaagct
     3121 gcctaaaagc ggtggatggc tgaacagcgt gaaggtggtg ctgggttttc tggaagtggc
     3181 actggcattc aaattctttt ccaatgccga cctggtggct cacttcggaa ttctgaaaag
     3241 agaggtcttc ttcatcatct ggacattatg cggcatcggt attgtgatct acctgctggg
     3301 atggatcaaa tttccgcatg acagcccggg taaagtgaag atcggtccgg caagagctgc
     3361 attcggcatc atcttcctgg cacttagcgt gcactttggc agtggcatcc taaaaggggg
     3421 taaccctttc ctggcaagtg gatttgcgcc accgatgttt tatagcattt atccccagga
     3481 tacccattgt ccgctgggac tggactgctt tcatgatttc gatgaagcgg ttgcatatgc
     3541 caaagaagtg aataaacctg tcatggtgga cttcacaggc tgggcctgtg tgaactgccg
     3601 taagatggaa gaacatgtct ggcctgaaga cgaggtttac aagctcatat ccgaagagta
     3661 tgtcctggtc agtctgtatg tagatgacaa agagatgctg ccggcagacg aacaatatgt
     3721 cagtgctttc agcggcaaga agatcagaac catcggcaac aagtggagcg attttcagag
     3781 cagttatttc ggtgtgaatt ctcagcctta ttatgtgctg atctctccgg atggtcaggt
     3841 gctgaacaac ccggttcctt atacaccaga tgtagaagaa tatgcctcct tcctggagtg
     3901 tggcctgagt tccttccgcg aagctgaaca ggtagctgaa aagtagttca gtgcagaaga
     3961 acgaatccgg cccagtagta ggggttggta tatcgttttc tgaccagagt tctggctgtt
     4021 tgaaatgcag tttctatacc atttccatcg gcaattgact ggtaaaaata ctgcatgaaa
     4081 taggccgtct gaacatctgg cactgtccac aaggtcatca gcaggtaatc tgcacctgct
     4141 gctttgaacg ccctttgaag tccataaacg ccttcatatc ctgatgcctc accgaggcct
     4201 gtttcgcagg cagacagcac caccagtctg gtatggggta aaaagagatt ggccgcttct
     4261 ctggatgtca ggataccatc ttcatgtccg tgtgcaggct tgcctgacca ggcggcattc
     4321 gcaccgctga acagcaatcc ggatcggtcc atgacatcaa gttcttctcc atgatccgga
     4381 ggaataaaaa atccatgcgt agcaatatgg agaatatcag gcggattttg cgggcctaag
     4441 gctttgatct gttcctccac agcttctgct ccggagtaag ctaccacatg cacaacggcc
     4501 gattcaagtt tactgatggc ctggatctcc tgaaaggtgc cgggtagaaa ggtccaggtg
     4561 gatgaacctt taaggcttcc tgataaaccg ggaaaagacc ccggttgcgg aaaggatgcg
     4621 gtggcctggt tccattcctg ttcgctgagg tcatactgca taccaccaaa agcaatgata
     4681 ttatgaatgt tgccagcacc ataaggggtt tgggtaacta cagacaatga tgaacaggtg
     4741 atgaaagtgt ttctgtccag taaccgcctg ccggatccta ttggtatgac atccaggttc
     4801 agctgatgca tcatgcctgt tgccgtgtag aatactgtat ctccctctgg tatccattct
     4861 tccagtggtt tccaaaaggt ttccagcaaa tgtgatccca tataaactgc aacatcttcg
     4921 tccagttccg gccaaccata tagttcatcc atacccgact caacagactt gttcagtaca
     4981 gttcccgtca gttgctgcag ttccttttct gtgccgatcc tgatcagttg cggatgctcc
     5041 atatccttcc gcaaaatgat ggccacatag atcacactgt ctgtccatct gttgccatca
     5101 taatatctga agtttgtgta ttcaaccgct gtctcctgtt caccaagtgc agacctgata
     5161 tctgtccacc gcacactcag cagatcgccg gtggtgcgca tggatctggc cattctgtac
     5221 agatcctttt ccatgaggtt gttgcgcttc tccagtgccg ccagaccgcg gatccttttg
     5281 tcaggaggca atgcatactc acgggctatt ttctccttgt tttcagtcca ctcatccaat
     5341 agtctgactg ccgaagtatc accaccggac agaatggttt gcctcatatc gatactgctg
     5401 ttcaggatca ttccacgagt ggccagtgca aggtccatgg cctgatcagc aagctcggga
     5461 aatctgtcag caaaagaata gcaaaaactc tgatgcatat acaggttctc gcgataccgg
     5521 ttcacatact ccgtttgttc agcttccgac aaaaatccaa agctttcgtc cagatcctgc
     5581 agaaacaggg tgatgatctc cacctggttt ctgatggcat actcccagtt ctgtgcagaa
     5641 acggtcaacg ggaataacat caatacaatt cgtataatta ttctcatgct aaaatcataa
     5701 aaaatgccga caaagagccg gcatcaaaat tatcgcagaa tccgttaaat ggattctggc
     5761 agcgtacagg ttctgccggc attcacccgt tggagatatt ccatcagcgg accgaaatag
     5821 tccaccatag gtttggcgct catatctgct ccgatacttt tcctgatatg ctcccgccag
     5881 tcaacggtgg caccggtggc catgacacca gacaggaatt cacctgtctc tttactaccc
     5941 cagtagttgg tggcatgcgg gtcctgatgc aggatgttcc gggcaatata atcgtgaaac
     6001 tggaagagca gtacattgct gatactgtag tcgtaatact gcgcagcgtc atcattgata
     6061 tgggtcttgg tggcggcatc acaatattgt gcaccacgtt ctgagggagg tacaatgccc
     6121 tggtatttct tcaccagttc ccaccatttg gcgttgtatt gatctgccgg caggttattg
     6181 gcatacagtt catattcgaa ttcggtcatg acaccgcttc cccagggtat gtgcacgatg
     6241 aaatccagtg cttccttcag cagcagcaat gtatcattgg tttgcaggcc gggttctatc
     6301 agtccgaggt tttccaggaa aggtttctgc agagacgcca ggccgatcat agatccgaat
     6361 gcttcatgat atccgcggtt ggcaccacct ctcagtacca taggtacatc aggggtggaa
     6421 taagacatgt aataatagat atggccgagt tcatgcagca ccgtactcca ccattcggtg
     6481 ttggcttcta cactcatgag cgaccgcaca tcatcctgca ggtccatatg ccaggcagag
     6541 gcatggttat tctttttata atcagcatct tccggcagcg ggtacaggga agacttgtcc
     6601 cagaaggttt ggggcagttc aggaaaaccc atactgaccc agaaagcttc gccttgttcc
     6661 atgacccatt cagcaccctt ctctttgagt gcgggatcga tgtccaggcc ttccacgctg
     6721 accatggcag tccagtcttg tccccagcgg ttaggcagcc agtgcgccgg caggtagtca
     6781 ggaaccggtt cgttgtatct ggccgcgagt tcatacctgg cccaggtgtg cagttcacgg
     6841 taaagtggcc atacgtcgcg gataagctgg tgggtcagct ccatcatttc ctgactggtc
     6901 atgccatatt cagacacctg gtaagcaaaa aagtcatcat aacccaaagg ggtaacactg
     6961 gcatttcgca aacgctgcag ttcagccaga ccatctttca gtgtggtgcc cacctctttg
     7021 cttgcattcc aggcatcgag tcgcctggat atgttctttt catctcgcag taagttgtca
     7081 atatcattcg tactcacctc tttgccatga agcatgtagt ggtaaccata gagcagttct
     7141 gtctggtggt tggtggcatc gatgcgctcc tttaccacct cgccggcaga tgccggattg
     7201 ttacctgcca tgaacagcat agcttccagc tgccgaacct gtaaaggcaa cagtttgtct
     7261 ttcatagcga ggtactttct gcagctgtca atattcgcag aactgccggt aaaggcagca
     7321 taggcttcat cagctctggc agcagcagca gatgcggtag tatcgccttc cacgatgcgg
     7381 gtgttcaggg tccactggcc ttcggcagat gcataataca acgcctggta ttcctggctg
     7441 tactgatcca gatagcgctg ggctgcagcc tgtacggcac tgttatcttc ctttttttcg
     7501 ccggcacagg aggccagcaa aacggcagga atgaacagta aattggtcca tttgttcatt
     7561 tttggcaatt atgattacta aggtacataa ggctggaaaa gcgattaaat ttttaacatt
     7621 cggttatctg ttaacaagct gtgtacatcc tttattcttt tcctaacttc gggggataat
     7681 tgtgtacttt cgcaagtatt gtataataat aatattattg attaaataaa tatggcagaa
     7741 gcagcagaca aactgaaagc cctggggctt gccctggatc agctcaataa gacctacggc
     7801 aagggtacgg tcatgaaact tggtgaaagc cccgagatca atgtagaagt gatctcttcc
     7861 ggttctatcg ggctggacac ggcattagga attggaggtt tccccagggg gcgaattgta
     7921 gagatctacg gaccggagtc atcgggtaag accaccctgg ccatccatgc cattgcagag
     7981 gtgcagaaaa aaggtggtat agctgccatc attgatgcag agcacgcgtt tgacaaaacc
     8041 tatgctgaaa agctgggggt agatgtggaa aatctgctga tctcacagcc tgacaatgga
     8101 gagcaggcgc tggagatcgc agaacatctg atacgatcag gtgcactgga tatcgtggtg
     8161 atcgactcgg tggctgcact ggtacctaag ggagagctgg agggcgagat gggtgaaagt
     8221 aaaatgggtc tgcaggccag gcttatgagt caggcactgc ggaagctgac aggaaccatt
     8281 aacaagaccg gctgtacctg tatcttcatc aaccagcttc gcgaaaagat cggtgtgatg
     8341 tttggcaacc ctgaaactac cactggtggc aacgcactta aattctatgc ttcgatcagg
     8401 ttggatattc gtcgcatcgg acagatcaaa gagaaggatg aagtgatggg taaccgggtg
     8461 cgtgtaaagg ttgtgaagaa taaggttgct ccgccattcc gcatggccga atttgatatc
     8521 atgtacggcg aaggcatctc taagctgggc gagatcgttg atattggtgt ggatgcgggt
     8581 atcctgcaga aaagcggttc ctggtacagc tatgacagca ccaaactggg gcagggtcgt
     8641 gaaggagtga aagagctgct gcgagacaac cctgaactct ctgatgaaat agaaggcaag
     8701 atcagagaaa agctgaagga aggcaaatga tcatttcgtt ctgataacag aaaaggccgg
     8761 caaatgccgg cctttttctt tagatgtttg ttgtgattac ttcacaaact taacagtcag
     8821 tttttcttct tcactgcgga taacagctac atacatacca ttaacggcaa cgttaccggc
     8881 tgtagcgata ccatcccata caatgctgtt ggaaccatta ccattcagta ccgcgatctg
     8941 ctgacctgca tggttgtaga tgatcagttc agttccgtca tgtgtaaata catcgccgaa
     9001 gaatacaaag ccttcaccca tgcggttcac agttacttcc tgctcttcca gcagttctgt
     9061 cagtgaacgg gtttctgtga tgttcagttc taccagacca aaaggactgg caccaactgc
     9121 attaggtcca acctggaagg tttcaccaga agtacgcaga ccgcagctac caccgctgta
     9181 ggtcatggta ttggtaccgc ttccgaggta accatcgcaa tagttgttcc agctcatctc
     9241 caaataacca ggggtccaga atgtgctgaa gaactcaaag ttatctacgt aggtagcaca
     9301 accttccggg ttgatcacgt tcatgtagaa gctttgattg aaacgatccc agtagccttc
     9361 tacatcccaa aggtaaccgg aaagtacatc tgccacaccg gtcatttccc aatacatagg
     9421 gccggtcttg gtagcagtta cctgataggt aaaaccatag gagtcagaaa agcaaagatt
     9481 ggctgcttct gccgtagttg ctccgatcag gccaattgaa atggctgctg cgagtagtgt
     9541 ttttctcatt ttgtgagttt taaatgggtg atataatgtt cctaaattag aattattaac
     9601 acaattacaa aaaaaagggg cagaaaattc tgccccttta catactattt aaaagaaatg
     9661 ttacttgaca aatttcacag taacatcttc attctcactg cgcattacgg ctacatacat
     9721 gcccgggtca gtcatttggc cgctggtagc aataccatcc catacaatgg tgttggaacc
     9781 attgccattt agcacagcga tctgctgacc aacatggttg taaatgatca gttctgctcc
     9841 agagttggta aacacgttgc tgaagaatac aaatccatca cccatgcgat tcacactcat
     9901 ctcttcttcc atcaggatct gatccagcgt gcgggtctct gttacctgca gtggataatg
     9961 aacagcagct gcttcatcaa atccaaccgt aaagatggag ttggagtagt caggttccat
    10021 agagcgacca acgcatgcac cgtttgtata ggtcagatca aagggtgttc ctgtaaatac
    10081 aacggcccca tcacaaacct gctcgtagtt ccagctcata gtacccggag cccaggcggt
    10141 tgaccagata ttcagggcat ctacataaaa tgtacaacca tcaggagaag gattggaaac
    10201 ggtgatataa aattcctggt tgaaagcatc ccagtaaccg tcaactggcc atggcaccgc
    10261 tgcacctacg cctatctcac cagacagggt ccagtattta ggggctgttt tggttgcgtt
    10321 gatctggtag ctggatccaa atgcatctgt aaagcagata attgtggcaa aagaactcgt
    10381 actgagcatt ccaattgcca atactgcaag gagtaatttc tttttcatat gcatgtttta
    10441 atgtgattaa ttactcctaa agtaaaaagt tttgacataa caagagcagt tggtgaaaaa
    10501 aaaaccagaa atactcaaat caggcagaca aatgtgacct acttcttacc caggtgtttt
    10561 agctgaacca cctctcttgg ggtgaggtag cgccagcgac cccgtggcag gtctttcttg
    10621 gtcagtccgg cataaacggt cctgtccagt ttggttacct gataaccgag gtgttcaaaa
    10681 atgcggcgaa cgatccggtt cttacctatg tgcaggctga tacctaccac gttcttccgg
    10741 tgggggtcgg gccaggcgat ctcatctacc atggcttttc catcttccag ttccaggcct
    10801 tcagcaatgg cttccatatc atgcttttct accgctttgt ccaatgtgac ctcatatact
    10861 ttctggacac cccggcttgg atgcgccagg tccatggcca gatcaccgtc gttggttatc
    10921 agcagcagtc cggtggtatg cctgtctaac cggccaaccg gatacaccct ttcctgggta
    10981 gctttggcca ccaggtccat gacggttctg cggtcacgat catcggttac agtggtcagg
    11041 taatctttgg gcttgttgag cagaagatag acgaagcggg cgggtttgac ctttttacct
    11101 ttataggtga cctcatcatc cgggtgcagt ttatacccca tttccataac cacttctccg
    11161 ttcaccttga tcaatccggc tttgatgagt tcatctgcct tacgccgggc ggccacacct
    11221 gcatttgcaa gaaacttatt cagtctcatg ccctcatccg ggcctttttt aggtttttga
    11281 atatcctgtt cccgaatgaa ctgacctttt ctcagtgttc ttttcggagc agctctctga
    11341 gcagatctgc ctttgaagga caggggttcc tgttcggtct ttttcttttt aggggaagtg
    11401 agtttgtctt tctttttggc ataatatgcc tgcttgcgtt ctctgttggt catagacgcc
    11461 agggcctccc gttcttcggg cgacatttca tccttgcgtt ttttagaaaa tctgagggta
    11521 cgttcgtgct gcgagcgctc acgcttcagt ttcgcctcct ccttcgtcag gggttgcgtt
    11581 ttctctttgg tcaccttccg ggtcttcttc tgacccgcct gtctgttcga tgttctcttc
    11641 atgttgctgg ttttgggcgt cttcccgacg ctcggcgctc accttgagcg taatggtatg
    11701 gtcgtcattc tcttcggcgc tgacgatgat atcgccgggg tcgccgatag agaggtcttt
    11761 ttgttcgaac tctttgagtt tgggcagttc atccagggca ttgataccaa aataatccat
    11821 aaagaactgg gtggtaccgt actgcagagg tttgcccggt gtatctgctc tgccggtgat
    11881 gcggatcaga tcacgttcca gcagtttctg cagggtgtaa tcgcagttga ctcctctgat
    11941 ctgttctata tcagttttgg ttaccggctg tgaataggca atgatgctca gacattccag
    12001 tgcagctgtt gacagtttgc gttttgcccg gtgctgcaac agggtgctga tgggtttgtg
    12061 gtacaagggt ttggacagaa actgatagcc tcctccgctt tgcaggatct cgaatacata
    12121 gtcatcgcta tcgtatttgg tcttcagctg gtcaaccaat tcctgaatgg cttccaggct
    12181 cacttcctct cctgagtagg tgaataaaac ggtttgcagc tctgccacag aaatgggttg
    12241 ttctgagctg aagatcagtg cttcgagtat ttgctggagc ttgtccatgg gttacaaaga
    12301 tagctgaaat gaatgaacga aaagccctga cgaagctgac tcagaagctt gatagccggg
    12361 attgcagcgg catcccgccc ctgcaatggt tcgagcgaag cgagttagca gtgcaggggc
    12421 gggatacagt ggaaagcccg acttcggtct gttaagggca ccccgaagct tgctccaaaa
    12481 aaaaaggcca ttcagatgaa tggcctgtga aacacatttt attatatatc aattaccctg
    12541 cagggctgcc gcaccactca cgatctctgt gagttctgtg gtaatagcgg cctggcgtgc
    12601 cctgttatag gcgatacgca gatctttcaa cagttcattg gcgttatcgc tggccttatc
    12661 catagcggtc atacgggcac cgtgctcaga agcgtggctg tccaggataa agcggtagaa
    12721 ggtggtattc aggatacgcg gaacaagttc gcctatgagc tcgtgtttgt cggggtcaaa
    12781 gatgaaatct gtctttgcct gtgtttcgcc ttcgtccacc ttgcgtatgg gcagaaactg
    12841 ctccgtttcc agtatctggg tagcggcgtt cttgaacttg ttgtagatca ccatgacctc
    12901 atcaaattct ttgtgcagga agccggtttt gatgtactct gtagcagcct ttacgtcttc
    12961 aaaattgagg ttgtggaaga ggttctggta ctttgtcagc agggtgtagt tgctgcgctt
    13021 atagacatcg taccctttct tgccgatgaa catcaggttt accgcggttg ccggcagatg
    13081 gctgtagcgt tcttcgatga cccttctggc cgccttggcc acgttggcat tgaaggcacc
    13141 gcacaaacct ttgtctgatg tgatgatgac aatgagggcc tttttcactt cccgctggtc
    13201 tgcatattca atacgcacat cgccctgggt tgcagcagca atattttcca gcatgctgcc
    13261 cattttgatg gcgtatggac gcatctgcag gatggcatcc tgcgcccggc gtagcttggc
    13321 agcactgacc attttcatgg ccttggtgat ctgctgcgta ctggttacgc tggtgatcct
    13381 tgtacgtact tcttttaatt taccagacat gctcctgtat taatagaact taagcagtgt
    13441 attttttggc catatcttta ccaacctctt ccagtttggc acgtacctca tcggtcagct
    13501 ggccggcttt caactggtcg agcacctctt tatggtgggt ttccaggaag gccaggtaag
    13561 tggtctcaaa ttccttgatc ttgtcaattg gcacattgcc cagcagcccc tgtgtaccca
    13621 ggtagatgat ggcgatctgc ttttcaaccg tgatcggtgc atactgcttc tgcttcagga
    13681 tctctacgtt gcgctttccc ttgttcagta cgttcatggt agcagcatcc aggtcagaac
    13741 cgaacttggc aaaggcctcg agttcacggt actgtgcctg gtcgagtttc agggtaccgg
    13801 ccactttctt catggacttg atctgggcat ttccacccac acggcttacg gagataccca
    13861 cgttgatggc ggggcgtaca cctgcgttga acaggttgga ttccaggaag atctgaccat
    13921 cggtaataga gatcacgttg gtcggaatat aagcggatac gtcgccggcc tgggtttcga
    13981 tgataggcag agcggtcaga gatccgccac ctttcacctt gtgtttgata ctttcaggca
    14041 ggtcgttcat attggcagcg atctcatcgt tgttgatcac tttggctgca cgttcgagca
    14101 ggcggctgtg cagatagaac acatctccgg ggtatgcttc ccttcccgga ggacggcgca
    14161 gcagcagaga tacttcgcgg taggctacag cctgcttgga aagatcatcg tagatgatga
    14221 gggcaggacg tccggtatct cggaagaatt cacccatggc tgcaccggca aacggagcat
    14281 agaactgcag cggtgcaggg tcagatgcag atgcagaaac gatgatggta tagtccatgg
    14341 ctccgtgatc ctgcagggtc ttggcaacct gagctacggt tgagttcttc tggccgcagg
    14401 caacatatat acaatacacc gggttgcctg cttcaaagaa ttcacgctgg ttgatgatgg
    14461 tatccagggc gatggcggtc ttaccggtct ggcggtcgcc gatgatcagt tcccgctgtc
    14521 cgcgaccgat cgggatcata gcatcaatag ctttgatacc ggtctgtaat ggttcattta
    14581 cgggctgtcg atagatcaca ccgggggctt tgcgctcaag gggcatctca aagagatcgc
    14641 cttctatggg gcctttgcca tcaataggtt cacccagggt gttgaccaca cgacccacca
    14701 ggccatcacc tacattgatg gatgcgatgc ggccggtacg gcgaacggtg tctccttcct
    14761 tgatctggtc agaggcaccc atcagaacgg ctcccacgtt atcttcttcg aggttgagca
    14821 cgatggccct gacaccattt tcgaaggcta cgagttcgcc tgatttggca ttgttcagtc
    14881 cgtagatacg ggcaatacca tcgcccacct gcagaacggt acccacttcc tccagttcag
    14941 aggcattgtt gaagttggac aactgctgtc tcagtatcgc actgatctcg tccggtttta
    15001 catctaccat tggtaagttt ttataaagca gttagaattt acgtatataa tcgttgttat
    15061 caaagtcgtc tttcaggatg ttcagaccac gctggatact ggtatcaata agcttgtctt
    15121 caaaacgcag cacataaccg ccaatcaggc tgtcatcaat ggtggtcttc atgcgtattt
    15181 cctgcaaacc tgccatactg cgaagacgat ccatgatggc ttgtctggtc tgttcagaaa
    15241 gttcataggc tgtgcgaagt tctacttctg taatgccttt atgggcattg tacatattgc
    15301 ggaacacatc gatcacatcg ggcaaaaatc cttctcttcc tttacgcacg aggatctcca
    15361 gaaagttgct ggtgatgctg gtcagtttat tgccgaatac accattgatc acagccattt
    15421 ttttgtcggc atggatgatg ggacttttca gcatcaggta aaaatcgcgg ctgtcatctg
    15481 tagccacgtt cagcatttcc atatcgcggt tcacttcttc cagctgaccc ttttcaatgg
    15541 ccagttgaag gatggatttg gcgtatcggt tggctattcg gatggcagac atggtcgttc
    15601 tttatcagtt tactttgatc tcatcaacca ggctgcgggt ataggcttcc tgtgcattgc
    15661 ggtcttgtag ttcgcggcgg agcactttct cgctgacctc cagcaccata ttacctacct
    15721 gatttttaat atcgatcagg gcagccatac gctggttatc aatctgttgc tgggcttctt
    15781 ccacgatgcg gttgtactct gtattggcct tttctcttgc ctcggcaatg atctgctctt
    15841 tggcttcttt ggcttcacgc agcattttag aacgctcttc acgggcttcg ttgaggatgc
    15901 gttcattatc ggccttcata ttggccattt cttcctttgc tcttacagca ctttgcagag
    15961 cctcttcaat acccttttca cgatcgccca gggctttgga aattgggccg aaggcaaact
    16021 tccacaggat gaagaacacc accaggaaga tgaacacggt ccagatgaac aaacccggat
    16081 ccggtgttac aaggtctaat gcatcgatca ggatgaacat gtatatattt ttttagtcgt
    16141 ttttttgaaa gatccggtgg caaccaaccg ttgccaccgg acaatctgtt ggatggatca
    16201 accctgcagc agggcaacca ccacggcaaa cagggccaca ccttctacca gggccgcagc
    16261 gataatcatg ttggaacgaa tatcaccaat ggcttccggc tgacgggcga tggattccag
    16321 ggcgctacca ccgatacggc ctacaccgat accggctcct acggctgcca gaccggcgcc
    16381 gatggcagcg atcccgttga tccctgcttc ctgaagaact gtcaataatt ccattgttac
    16441 ttgtttataa tgaataatag aatccgtgat caatgatgaa ctgcttcttc atggtggtgc
    16501 tcatgcttct ccatggccag cgagatgaag agcgcactca gcatcacgaa gataaaggcc
    16561 tgcaggaatg ccaccagcaa ttccagaaaa ctcatgaata tggtaaatgg tacggccacc
    16621 agggctccgc ccagacctcc tccgaggctt tcacctgcgt tgccgaagat gaagatcagg
    16681 cctaccaggc tgaggataat gatgtgtccg gctgtgatat tggcgaacaa tcggatcatg
    16741 agggcgaagg gcttgatgaa aataccgaga atctcaatgg gcacgaggat gatcttaacc
    16801 cagagcggaa cacccggagg ccacaggatg tgcatccagt aatctttggt tccgttcaca
    16861 ttggtcagaa tgaaagtgag cacagacagg gccatggtca ctgtaatatt tccggtcagg
    16921 ttgggattgg agatcagggg gatcaaaccc agcatattgg caaaccagat gaagaagaac
    16981 agcgtcagca tataaggcag gaaacgctct gtattgtcac ccagattggg tttcaccacg
    17041 tcatcacgca caaaaatgat gaggggctcc atgagcgact gcagtccttt cggcgcctga
    17101 cccggcctgc gggcataagc tctggccact gaggtaaata tccagatgag caataatgcc
    17161 gtcaggatga gtgtggctac gttcttggta acagaaaggt ccaggaaggc agtaccgtct
    17221 gcccgggcca cacggtcgtg caccataaca tagcccttgt aagaagcggg ctcatggtga
    17281 gcagcttcca tatcaggatg ctcctgttcc atgccttctt ctaccttccc catgtccatg
    17341 ccatgctctt cgtgggccat ttcggtctcc acttcttctg catgatgggc ttcgggaagc
    17401 ttgttggaca agaagatcgc cagcccgtct tccttgtggt acacaatgac cggaaggggg
    17461 atgctcacat tacctacaat atgccaggta ttggcatcag caatatggtg aagagcgaaa
    17521 tccttggggt tgtatgctcc ttcttcagcg gccatcagac tgccggcaga gaggagtaaa
    17581 atggcggaaa gaactacctg aatgatcttt ttcactggaa tttgttgaaa aggcctttca
    17641 aaaaccggtg caaaggtagg ggtttaccgg gaaaaaaaga aagccaaaca actaaattcg
    17701 cagctacatg aaagtcaaat tctatgcggt ttccggggca ttgacggccc tgttgctgat
    17761 actatcgtgc gggaacaaag cagggcgtga ttaccaggct gaaaataccg ttcctgcgat
    17821 caaacatccg tctgttaccg gcgatctgga aaaccagttc ctggaagccg gcctggttga
    17881 tattgccggg gttgtgccgg ggatccaggt ggacctgaaa tattccacca cagacaactt
    17941 catgcatacc gatatgtatg gcagtctgga aaaagcatac ctgcacccgg aagtagcggc
    18001 caaactggca aaggccgccg gtgcactttc tgattcacat ccggatcttc atttcctggt
    18061 atatgatggc gcccgccctc tcagcataca acaattcatg tgggatcatg tggaccttcc
    18121 gcccgatcag cgcaggcagt ttctgagccc gcctgatcag ttatcgctgc ataactacgg
    18181 agccgctgta gatatcaccc tggcaaccgc cgatggtctg gccattgata tgggcactcc
    18241 gtatgacgat ccgggtgtac tcagttaccc catccgtgaa tcagaatttc tggctaacgg
    18301 gtcgctctct gcagaacagg ttgccaaccg gcaactgctt cggtcagtga tgcgggaggc
    18361 aggttttacc ggcattgaca gcgagtggtg gcatttcaac agttgcgaca gacagttcgc
    18421 agccgctcat tataccctgc ttcgctgatc agatcttaaa tcggaatacc ccgattgcgt
    18481 aagaaacggt tggtttgctc tgtccgagaa acacgagtat gtctcgggtt gatacaccaa
    18541 actcccagaa gccgaggtcc acaccaatac cggcaggcac attgaaccca tagttgccgc
    18601 ccgcggtgag gccgccgctc aactgcagaa aacggagcag ggtcaatctg gcgccggcac
    18661 ctgcctggac agaactgaag tcttgtgcag tggaagacaa tggcaaagag atatcggctg
    18721 caagggtaag catgtcactc attttcaagg ctgctccggc tcttaatgcc gttggcagcg
    18781 gcacttcaaa gcgctctata ccattgtagt caagcaactg ctctccatcc agcagttcag
    18841 aaaccaggtc aagggtctgg gtgctgttga tgccattgaa atagatcgta tctacaatga
    18901 tgttgttgaa caccagggta ttcaggtcat atgacacctt tccgatctgc actacgctca
    18961 aacccacgct gagatcctca cccaggtctg cactgaagcc gatatcaaat ccgaatccgg
    19021 tacctaccgg ttggagtggc tgctgattcg gatcaaaggt ttcaatataa tccggcgaat
    19081 tgaccagatc cagacccaga gcggcctgcc ccacgatctc ttcgttctga ctgcgaaagt
    19141 caagccatcc ataagacatg atatagttca acccgatgcc ggcatagata ttcaggtgat
    19201 cagaagcaaa ccatcttctt ccccatgcaa tgctggcagc acttcgcaga ttaaaatgaa
    19261 ggctggttcc ttccgttagt ccggccagtg acaagggctc ataggccacc ccgtatatgt
    19321 tactggtgtc ttcaattatg atggtatcta tatacccttc gtagttaaat ccctgaaaaa
    19381 ggaggttact ggcttcactg cccagggtaa aggactgatt ggctgtaaca gacagaccaa
    19441 tggccaggcc gccggcgttt cccagatcga aaccaaatgc aaccggtctc acaaagacat
    19501 cggtctgaaa tccatcctgt aaaagtgttt ctgcaatggc cgcctgttgt tcctgtgtaa
    19561 gctgttcacc gccggttggc tggaaatacc tgacctcctc tttggtcaga gcttcggaat
    19621 agacagacaa gcccatacta cctatttcaa atgacagctt gtagtctgtg tcatacccaa
    19681 gatttgccgg gttgatacct atagactggt agtcacgcac caaggcagtg ctggctccca
    19741 tacccatata attgaacaac atgccatctg tctggcacca gctgagggag ctgatcaggc
    19801 aggcaaacag tgtaagcagg gaagctttca tcatttttct gtagtttatc gacaaaatta
    19861 caagatatgc aatccaactt tcgcttaacc ggctcactct tttcagtttt gatcgtattg
    19921 ctgtgtatgc cgctggccac atcggcacaa aagagtattg ctgtttcaga ggtacaggag
    19981 accatgagca tgggaaccag aaatgggttt caggcgattc tcagtgatca gtctaaaaaa
    20041 gacgtggaag ccgtgctgga agaccttctg aaggaatatg acaagaaagc caaaatggaa
    20101 aaatctggca agaatgaagc taaggtggat gatgtgatca ttccatccat aagtgaccag
    20161 ccggtagatc tgtattttct gttcagcgaa gacaaaagct cctgcacggt aaccggtttt
    20221 tttgacatgg gtacttcatt tctgtcatca caggtttatc cggatcggtt cagatcaggt
    20281 gaggttttca tgcaaaaact tggccttagg gcagaaaaaa cgaggctggc tggtctggta
    20341 aaggctgcta cagacgatat gcaaagtctg gaaagagata aagccggcct ggaaaaagac
    20401 aaagaaaaac tggaaaagtc catagctgac tgtgaagcca ccattgctaa agcgaaatct
    20461 gacctggaca ccaatgccag tgatacgaag aagaaatctg aggaaatagg tcaacagaaa
    20521 gagaaactgg gcagtctgca aaaagaactg gaccagtatc gcgatcactg agccgttgct
    20581 tcttctacat tgaggcggaa accattcccg tgaatgttca cgatctccag gttgggatcg
    20641 tctttcaggt atttgcgcag tttggtaatg aacacatcca tacttctggc cgtgaaataa
    20701 tcatcgcggc cccatatctg tttaagcgct tcttcccggt acaggatgtc gttcactttc
    20761 atgcacaaca tccgcaacag ttcagcttcc ttgggagata atttctcctg cacaccatcg
    20821 tgggtaagga tgcggttctt gtagttgaag tgatacttac caacctcaaa ctctttctgc
    20881 tggttgttct gtttgagcct gccccttttc agaactgcag cgatgcggta cagcaactct
    20941 tctgaattga acggcttggt gatgtaatca tcagcaccta tcttgaaacc ttctatctta
    21001 tcctgctgct gggtcttggc agtaagaaaa atgacgggaa tatcggggtt catacgtttg
    21061 atctcagcag cggctgtaaa tccatctttc ttaggcatca tgacatccag cacacacagc
    21121 ccgaattgtt ctgtgcggaa tatatccacg cccttatcac catcggtggc cagggtcact
    21181 tcatatccgt tcatggtaag gtaatctttc agtacggcac cgaaattcgg atcatcttca
    21241 caaagcagaa tgcggatctt gggttcttcc atgttggtca gttttgtggt aattgtatgg
    21301 tgaaagtgct gccttttccg ggtgcacttt taacttctat aatgccaccg tgtgcttcca
    21361 ctacggtctt tacataggtc aggccaaggc caaagccctt gatattatgg acatttccgg
    21421 ttggtacccg gtagaatttt tcaaagatca ttttctgctg atcagcactc attcccatac
    21481 catgatcctg tacactgatc agaatatgat ttcccttatt gcgtgtactg atcacaatgt
    21541 caggggtttc aggcgagtat ttactggcat tatccagcag gttgtagatc acattgacca
    21601 ggtgtacatt gtcgccggta aagagggcat tgtctgcctc cagtctcttg gttatcctgc
    21661 cctgcttttc ttccaccagc aaagtcacat tgtccactgc gtgttctatg acctcatgca
    21721 gatccagttc ttccggtttg agattgagtt catgtttatc cagcaatgcg gcctgaagta
    21781 ctttttccac ctgaccgttc attcgacggt tctcttctct gatgataccg gtatagtgtt
    21841 tgacccgttg ttcatcctgc aataccagcg ggctgttgat cgcatctact gccagactga
    21901 tggttgcaag aggcgtcttg aactcatggg tcatgttgtt gatgaaatca ttcttaatat
    21961 ctgagagttt cttctgacga agaatgatat gaatgctgta gctgaataca aaaatgataa
    22021 cggaggtgaa cagcagagaa cccagcaaaa ggacccatat ggactgaagc agataggtgc
    22081 gctgatcagg aaactgcacc atcagcaggt ctttaccaaa aaagatattg ttgggaaaaa
    22141 ggctggcagt atgctgggtc ttcagtaagg tggatttttg ttcatcagcc actggcgaga
    22201 tcagggtctt accattcaac agaacgccat actggaattc tgtttcaata ccccggtttc
    22261 ggaactcaag ctgcagcatt tgagaaaggt aagcagtatc caccttatcg cccggctgcg
    22321 ccccctgcat gatcatttcc acgatcatct gctgcatgac ctggtccagt ttatctgcgc
    22381 ttacctgaaa ctgtttatcg atctctttga gtatggtgga gatgatctca tcattgcccc
    22441 cttcctgccc gacccttgta cccatctcta ccggctccag tatcattgtg ggccggttag
    22501 gcagagctcc gggcgcagga taactgtacg atgctccggg ttcggtcagt gctgcaatat
    22561 tgatgtgctc tgactgcata gcttcctgaa tcagggtatc gcttacccag gttttattga
    22621 tgcgcatact accgtttacc gcaaaagcgg aatcactgaa gatgaggtcg gtatggctct
    22681 tcagcatggt ggcgatccag tgggtctcta cattctctga aacccttgtc agggcatctg
    22741 ctacctgata gccgaagatc ttctccttct ggttgagggt gctctgaatc cagtacacct
    22801 gcaccaggat gataccgagc aatgcggtac tcatcagggc tatgatgatg cggatacgag
    22861 cctgtttcat gtggccaaaa ctacgatatg ttaaaacgcc gaaaaacctg tttaacgtgg
    22921 ccttaacaca atttagttgt gcattaactt ggagccgaag acagcagaaa tatttttgtg
    22981 taaccggttt gcagaatatt tttcataccg gcatccaata agtttaaaag tacacacgat
    23041 gaaagcgata accaaatgga catcaggcac cgtcatgatg ttggccgtat tgatccagct
    23101 acctgccagt gcacaggaaa atgatcagat ccggttgaag atcgatgcta acgtaaatgg
    23161 ccagcatatt cagatcgaca cgaccatcga acagatggaa gattttgacc tgcagcaata
    23221 tctgcgtgaa ctaggtctgg aagaggagat ggacgggctg cagaccatgg atattcgcat
    23281 ccgcgacaac agcgaccata ccttcttcaa ttttgatgaa ctggaaaatg ctgaatgggc
    23341 caatgacctg aaactccatc tggaatccat cgacatgccg gagatgcctg aactgcccgc
    23401 catgggccat gtcatgttct ttaacggaaa caaagcattt ctgggtgtgg taactgacaa
    23461 tacaaagagc agagacgggg tgttggtcaa cgaagttgta gagaacagtg ctgctgctgc
    23521 agcagggttg cagagaggcg atatcattac catgatcaat gacaagaccg ttgaatctac
    23581 caataacctg atcgagatcc tttctaccta tcagccggga gaggtgatca acgtgactta
    23641 cctgcgtgat gacagcaaac aacaggctac agctaccctg aaagaaaacg aaggatattt
    23701 tgaatctgca gaatgggaac agtacggtga gcgatgggaa gaatggggcg aagcttttga
    23761 aaagaaaatg gaaaatctgg gtgatgattt ccagatgcga tgggaaagca atatggctga
    23821 tgtgcagaaa ccttttctgg gagtttacct caacgaacat gatcagggag cagagatcac
    23881 aggtgttagt gaaggaagtg ctgctgaagc agcaggcctg cagaaaggcg atatcataac
    23941 cgcaatagac ggcataacga tggagacaac ccgacaggta tctgattaca tcagaagcaa
    24001 gcagatccat gacgaagttt cagtcacagt attgcgccag ggaacttcca tacagatacc
    24061 agctattctc ggtaagcata acagtgatct gttattctgg atggatgatg aaaaaggggt
    24121 tagccctttg ggtaatgaag aaaggcacat attcttttca cctggaaacg gaaactgtca
    24181 ttcttattca tatgagctga atgacgatgg aaaaaagacc atcatcatta ccatggatct
    24241 ggaaaacgaa caatcttctt caacagacag tgctcgtaca ggaaatgtgt ttcgtgcaga
    24301 agatgtagag ttctatccca accccagcaa tggcaccttc aatcttcgtt ttgcagttga
    24361 agagccttca gatgtgcttg ttcagatacg tgatgcccgc ggcaacatgg tgtatgaaga
    24421 aaacctggag aattacagcg gtacctatga ccacacgatc agcctgggca atgaagccaa
    24481 ggggaattac tttatcaata tcgtgcgggg tgattatatc atcaccaaac agatagtgat
    24541 ccaataagaa gctgtcttaa gttgttttgg ccggtgcatt gcaccggctt tttttatgcc
    24601 ggctgaatga cctgtaacgg aatgaagtct gcagggttca gataagtctc aaagaactca
    24661 tcacaccata gacatacatt cttcagtgcc agtgttcttt catgcgcata cgcctctgag
    24721 ataccgaatg actcgcccat cttccaggga tcaccttcat tgagtcgttg aaagaccgga
    24781 ttggaggcta tgcgttccaa cacatctgca accttttcgg tccttgcatc tcccagggga
    24841 aacctggtgc ccggacagca cgggttgatc ttccacaact gtatgctgat ctcctgcgga
    24901 atatcatcca caccacctaa aaatccgatg gcgccactca ggatgttgca gaagttatgt
    24961 tccggatctt tcttccagat gtctttctcc atcgcccttc cgcgtgccca gtttcctccc
    25021 agccacatat cttctgtagc accccagaaa ccaaaactgg ctgactgttt atgcagatag
    25081 gtgtcttttt caatcagcgg actggtgttg tcatcggtca tgcctctgct cagaaacaat
    25141 tctttaagct gatctatcct tttacctgca tgcttgtggt atctgtctat gctggcaatg
    25201 tcaatacggg aaacaccttt ttccagtaaa gtatccagga tctgaccggt catgagatca
    25261 ccattggttt gcagcatgat ctgggtactg ctgccatacc tttctgccac ccgatccagc
    25321 agaaggaaga gctgtttacg ctcagcaagc ggctcacccc cactgagtat caaacgatcc
    25381 attttctggg gcaggttgtc aatgatctgc agcacctcct catcctgcaa tctggcaccc
    25441 cgggggccac tcaggttgta acaatggtcg cactgatcat tgcacaattg agtgaatacc
    25501 cagtacacag attctatatg gtcaaaacga ttcatcatga ttggttatac tttttccaga
    25561 atgccatttc atcttgtccg cccgttcttg ggtctgacag gaatgcgccc gtatcaggat
    25621 acatggtcag cacacccgac tccataaatg cagtttgctg accggtcaga atggcctctt
    25681 tcagatcatg tgtgacaagc aggctggtaa ttctatgcgc agtacttaat cgcataaata
    25741 attcctgcat ttgttttctg gtgtccggat ccagattacc aaagggctca tccagcaata
    25801 atacagaggg gtgtatgatc atggccctgc caaaactgac ccgctgccgc tggcctcctg
    25861 acaactgatg cggacgtttg tgaccctgat cttccaaacc cagttcagcc agcatttgct
    25921 ccgtttgccg gcgtatatct gaagcgggta ctccacgcac ttccaggcca taggcaatat
    25981 tggccgatgc agtcatgtgc ggaaacaaca atggctcctg gttgagatag accgcatttc
    26041 gtttatgtac cgccacacca ctcatgttct ttccattgag ccagatctcg ccgttgtcag
    26101 ccagctccaa tcctgcgatg atgcgcaaca aagtggtctt gccgcacccg ctcttaccga
    26161 gtactgccag tgtatgacct tcctccagca ccaggctgat gtctgtaagg acctgctgcg
    26221 caccgaatga cttgctgata tgtttcaatt ccatcaaagg ctgacctctt taaaaacaaa
    26281 acgccggttc caaaaaagca atatggcagg tggcaggatc aaaagggtag cagataaagc
    26341 cgccatagaa gggtttgatt cctgaatata cagaaagacc ttgagtgtaa gagtttgtat
    26401 ctgaccggta ccgatcacag aagtgagtcc gtactccagc caggatataa taaaggtctg
    26461 aaagaaacag accagcaaaa atggcctggc tacaggccag atgatatacc ggtatgtatg
    26521 cacaggagtg cttccaagtg tggcactcag ctgctcaagc ctgagtatcc gaacactcca
    26581 gaatgaatga aaatatatga tggcaaaagg cagggcgatg agcatttgag caaggatgac
    26641 cccggctacc gttcctgcaa gacccgccac ctgaaaatag aataacagaa tggtagcgaa
    26701 taataccggc gacatggtga ccggcagcag actgaaaaac aatccgccct gccggaatga
    26761 cgaatagctt accgccctgg ataacagcaa tccgccagtg gaagccaatg tagcagtaca
    26821 ggtcgctatg cccaggctta gcagaaaact tgctcctact gctccctgac tatgcagaaa
    26881 tgaaccccag tgctgaaagg tccaagtttc cggtaagaga gcaggaaaat gccagcgccc
    26941 aaagaaactc atgagcggca ggataccata aggtaaggct gccagaatga tactgactac
    27001 aagacttgca tatgtttttc ttctatgcat gtctggcatt ttttcgaagc agtaacatat
    27061 tcagcagcag cacaagcagc acatagctta ccagcatggc ataggccagg ggcttgtcca
    27121 gcaggtcgaa ccgaaacatc ttatcagcga tcagcggcga tagcatcctg ggatctgata
    27181 cccccagcag taatgggatc tcataacttc ccataacgaa cagcaggaac atcaggatcg
    27241 atggaaatgc tgagcgcagc aacaacggaa tcacgatagt tctggacgcc tgaccatttt
    27301 ctgcccccag attcctggct gtaagcaaca gcatatctcc ctgttcctgc cggtaaatat
    27361 ttctgaacaa agctgtaaag tatggtaccg ccagaatgat atgtgtgatg atgataccta
    27421 ccgcaagcct gtcattgatc agtgccggaa atgcatccgg accagcggtc agaccaaccg
    27481 cataggtgag tctggagagc cagcccgaag gtgataacag ctggtaggtc atcaacgctg
    27541 ctaccactgc cggtatcccc aatggcagaa gcagtatgaa tgagatgttt cttcgttgca
    27601 acgtggtgcc atatcgcagt gtcaaccaga gggcaataca aactgccagg ccaatgctga
    27661 tcactgcaat gatcatgcta tacagcagag agaaaaagaa aggactattg gtccggagtt
    27721 gctgccagta ggctattgtg tatccatctg atagtaaacc ggtcaacccg acactgtaca
    27781 ggaaagcata gaccaggctt gcacttaccg gcaggatcac cagtaagaca aaaacgatcc
    27841 acgctatgga tttacctgcc ttcaatgata atgtttctga aatcgtcaaa taacctgatc
    27901 atatattccg gactgagttc cataagagca taccggctga tactggcccg gtcaggagca
    27961 tattttcgtc ccggaatttc ccggaattgc tgaggccagt tgtccggtaa ttcgtctgtt
    28021 cgaagcacgg taccatcgcc ccacacagca ggctgcatct tttcaaactg tgcttcaggg
    28081 ctgatcagaa aattgatgac catcatggca cccggcaggt tgctgcttct ttgtgtcagc
    28141 cccaggtagt gtgaattccg tatagtaccg gaggtgaaga cataagacct ggctgtttcc
    28201 ggcaaaatgc cctgcaaaac tttgttgtca acttccccgt cattattact catgctgaag
    28261 tctatctcac cgttggccaa catcctgtgc atggttgccg gaccatcagg aaaggtcttg
    28321 ccttccttcc acatataagg ctgcaatgcc ctgatgtact cccataactg cgcactgtag
    28381 tgctggtatt tctgctcatc aaaaggacct gccaacacag aatcgccacc ggcaatggct
    28441 atgagccagc tcttcagaag agtcatacct gtaaactcat tgggaatggt gaaacttccc
    28501 ggatgcgcct gtacataggt cagcagctcg gtcatcgttc ggggaggttc cggtgtgcgc
    28561 aggctgtcat agatgatgga aagctgcaca ttgccccacg gacattccat tccattgatg
    28621 ggttgctgaa aatctgtggc aatgtaggga ttatcccagt ctatgtatcg ttcgttggga
    28681 aggatccctg aaaatggccc gtacaaagcg tctatctgcc gtagctgata aaaggtctcg
    28741 ccgttgatcc acatcatatc tatggatgac tgcttttttc cggcttccag ttctgacagc
    28801 agcaatgata cgatcatatt tccctgacct gaagagatct ccagttcgat gccatacagc
    28861 tctttcagtt ttggttttac atattgctgc atgtaggtgt taatgaacgg atcgccttgc
    28921 cacatcatca tgcgaaccgt gctgcctgtt ccggctgcca ccacttcatc ccacgatccg
    28981 ggaactgttg cctgatcccc tttccggtgg catgatgaaa ataacaacgc aataaataat
    29041 atactaaagt acttcataaa atttagataa caaggggttg ccttcgcaac cccttgtatg
    29101 atcagaaata atatcgtgcg ccaaactgga actgtcgggg tggtgccgca ttgcggcgaa
    29161 ccagcacacc ggaatcagcc ggacctacct gcacctgatt gctctgggta gcgttattgc
    29221 tgtatccact caggttctgt gcattcagca gattgaaaac gtcggccctg aactccaggt
    29281 taccggtacc caatgaccag gtgtattgtg cacccagatc tatggtattg ctccagggca
    29341 ggcggtcatt gttccggctc tcgcccggac tgcggtcact gttgcccaca taggcatcgc
    29401 cgaaggaacg gccatcacca ttcagatcag tggtgccgta aactgatgca tcgggaatgc
    29461 ggttgatggg cataccactt tgaaccagtc cggcaaatgt gaactcccat ccatcgcacg
    29521 ggtagagatt gaaaatggta ttgaacacat gggtgcggtc gttgatactg gggccccatt
    29581 cgtcttcaaa gttattgctg tccatcgccc ggaagttgat gtcttccgtg ttgttctcat
    29641 ttcggctcag ggtatagatg aaacgccatg cgtaattatc gttcatccgt tccttctgca
    29701 ggttgagact gatgccataa taccttgctt caccggcggt ctcactcatg accacgttac
    29761 gtgccacacc atacacggta tcaccattga tgatggcata gcttccttcg tagatcgcca
    29821 ccggacgggt caggtctgcc gtggcctgat cacgaacgtc acctgcttca aagttctcat
    29881 cgaccggata tgcttccgga gcattcagat ctaccagcct gtacaggaag ttggtcttgt
    29941 tatagaagcc atctatatag aaaagcttgt cttcggtcag ctgcacctgg tagcccagag
    30001 atatctggtg ggtttgcgga ttctggtagc cgttcggatt caggatgcgg cgttcattgc
    30061 tgaatgcctg gtcacgctgg ctctgtaggt catctgctga ggggccgttc agatatggca
    30121 caccagatgc aaaagcagag aggtttcctt caaaagtgat cttgtccaga tcggttgttg
    30181 atggcagtat cccctggtct atcagtgcct gcaactgtgc cctgtagtca tcgctggtag
    30241 tgttctgctg cagtgcatcg ctgtagatgg catagaggat cttatcgtac accatagcat
    30301 agccaccgcg aatcacactg cggctgttca gcttgtagtt gaaggatgca cgcggggcga
    30361 tgttgttgaa atcgccctga tcgccaccac ctttactcag gttgtcataa tcataccgga
    30421 taccataggt caggttaagt ttgttgctca cactccatat gtcttctgca tacacgctga
    30481 aaatattctg gcgtgtgccg aaagtggcag gacgcagctc cactgcatat cctagtacct
    30541 gcacatcaga tggaatatca ttgacatcca gagctgatcc aaaagtggcc agctgggcca
    30601 gctgatcttc ggttacctga acggtgtaac tgccgtcggg gttgccgcca cctgccagct
    30661 ggaagtctga gctgatcact tccacaccag ttttgaatgt gtggttctcc cggtagatgt
    30721 tgagcttctg ctggaactgc agggtatttt ccagatcatc aaaataataa cccggatgcc
    30781 ccagtattgc caccgtcaat ccatcaggtc caataacgtt ggtgtttggt cctgctccgt
    30841 tcacaggatc tgcgtagttc cagcggaaac ggctgaactg caggttggtc tctgtgctga
    30901 aaccatcgcc cacatagctg ttcttgctgg ccagcaggaa agaattgcgc atttgggtat
    30961 tggctgccga aggaaatact acgccgccct ccagtccacc gccctgtctt tcaattccca
    31021 ccagaccaac atttgcccga agggaagaac gaaagcgttt attccatacc tgatctattt
    31081 ttccgctcag gtaggtgaag ttgttacgtc ccggaacggc ttctgtcagc cccagctgag
    31141 gaacgtccag cacattctct ttcaggtcaa aggtttgttc agcatttaca taatagaagg
    31201 tcctatctgg tttgatggct ccgccaaatc ctatacccag ctgctggcgc atgaaggtat
    31261 tgttcacctg attaccgctc agatctcgtt gggtataagg tgatgcacct acacccaggg
    31321 tatctatcac agcaccgggg cgtgtcagat aaaaagcttc tgcagaaaaa tcgttggagc
    31381 cgctgcgggt ggtgatgtca atgatgccgt tgccggtcag accgtactcg gtactgaagt
    31441 tgttggtcag cacagaaatg ttgcgggcaa agcctacggg gatggcaaat ttctgcccgc
    31501 ccagaaaacg ctcattgtta tcgaaaccgt cgatggtgta attggtgtac agtgagttac
    31561 cacccatgat gctcacgtta ggtgcttcgg gatagaatcc ggttgcctgt accacgcccg
    31621 gaagcctgaa cagaacacgg gtaatatcgc gaccttctac cgggatctgc ctgatctctt
    31681 tcagtgaaag ctctgatgaa acctccgcat tgatcgtatt gatgcgggag gtactggccg
    31741 cagttatggc cacttcatcc agtgtggtct cggtctttct tggcatggca agttgcacac
    31801 tggccttttc attgtttttg agctctactt cgctcaggct gacaccactg tagcttgcgt
    31861 tgcccgcagc agagatggta taggtgccgt tcacgggcag tgatctgaaa cgcactttac
    31921 cctgagcatc agttactgac tggtctgtat aacctatagc agtgttctga atactgacgg
    31981 taatgccttc cagaggtcct gcagatgcca ggtcggtcac attgacctcc agatcgacct
    32041 gggctattat agttgataca aataatatat gtaataaagt taatgcaaat gttcgcataa
    32101 cattggtttt aggtgtaaaa aaaagctggt tggaaaatgt caggcaagta caggcggacc
    32161 cctgcgaata tgcacccggt tcatatagcg ggtgtgaggc aactcaggta aattcttttg
    32221 atctgatgcc tggatcagga aagaaagaag ccactgcaga ccagatggtt tgcggatcct
    32281 ggcaagcagc cgttgtatat cctgcaaaga atgcaggtcg tgtagtctgg tgagcagatt
    32341 gacggtagca agttgcatca gctcctttag tacgaccggg gtctgccagt gtatgttcag
    32401 aagatcagcc tcgtcaaagg tctttctgtc tatagccatc agatagaccc ctcctctgtg
    32461 gtcgggtccc agtaccagtc ctttatccag tctggaagct gcctgcagca gatggtgact
    32521 tcgcaggaac ggacaatcgc tgcctacagt gatcacttta tcaaaacctg cagcaaatac
    32581 ggtagtaata gcatgatgga aacgttcgcc gaagctttgt ccctgctgtc tggtggaatc
    32641 aatgacaaat accggaagcc cggtggcatg cgcttcagaa catatacttc tgaacaatct
    32701 ggatgccagg gtagtattgg cccgatgtct tcttgcaccc agccagttct tatgacgggc
    32761 ctcatcttcc ggatgccggg caaaaacgag aatagcagta tcagtacacg atcggtccac
    32821 gaaaacaatg gctatgtggc tgtaaatata caggagcaga aattagttca gtaaactctg
    32881 ataaactatt cagttgcgga tcatgatccg gtagctgtaa ggagctgcac tgaaagcatc
    32941 ctgcagcacc agttgatcgc ctgttaagag atctacccac acaccgcgaa gggtctccgg
    33001 gcaaaccgta ctgatctcca cgtctcttgg attgatcatt accaacagtt ggtcgttcag
    33061 ccaggatcgg gtaaaaagga tcatatgata tcctgtgtca aaccactctc tgtttcctct
    33121 tcgtaacgcc ggatggatac gatataagcg catcagatcc tgataggcct gtaagatctc
    33181 cggattagaa agccagttgg tggtggactg attgaaaaag gaaactgttc ctgttcgtcc
    33241 cacttcctgt gtaccataga tcaatggcat accggcacca ctggtcaggg taagtgcaaa
    33301 tgcggtgagc gccgcctcct gactgccaaa taaagtaggt ggtgcagcgt cccaggctga
    33361 ttcatcatgg ttcgtggtaa ataccagccg gttatgcaat ggggcagttg agctggccac
    33421 cggaagactt cccacatctg cattactgtt catgacgttt ttgagatcac cgtaccagct
    33481 ccagccaaaa ttcagatcaa acccggctgt caggtgatcg ggtctggcac cttctgccag
    33541 catgaacaaa gttttatttg ccctgcttct cagagaatcg ttcgcctgct gccagaaatc
    33601 aaacggcacc agatctgcag catcgcatct gaaaccatct atgcctgttt ctgttatcca
    33661 gtaccacatt gcatcgatca tggccagccg catgttgttg ttgctgtaat tgagatctgc
    33721 cacatcattc catccggtgc cgggtggaga aatgatattg ccactgccat cctgggtgta
    33781 ccagtcgggg tgttcagtga tccaggggtt gtcccaggca gtatggttgg caacccaatc
    33841 caatatcact gccatgccat gtgcatgtgc tgctcctaca agttctgtca gtttattcag
    33901 atcgcccagg tcaggattga cctcggtata atctgccacg gcataaggtg agcctactgc
    33961 attcacctgc ccttccggat aaatgggcat gagccagatc acattcactt ccaggctatc
    34021 cagatcgtcc agtctgtcta tgacagcctg caggttgcgt gtagggctgt aggctttgaa
    34081 atttacttca tagaccactg catcctgcag ggctgtaagt tcgaaaattt caggcgagat
    34141 cgtctcctta ctacaggaaa agaacagaac gatcggaaaa aacagaaata aaactttctt
    34201 actcatgcct gtgtagatcc ggacaggaga tcaccgggct tcgttaccca gatcttccag
    34261 ttgttcagac agatcatcta ttgcatcacc catgtcatcc agggtttcct gcaggtcatt
    34321 cagttcctgg gtttgctgtt ccatatctcc catacccgga atagtaaaat ctaccagcca
    34381 tttaccatct actttaacaa gacgataaac agattcatta ttgaattcat caagtgtagt
    34441 gaccgtagct gagtctccgt tgatgaccgt tttgtcagac aggatctggt attgatacat
    34501 ttctacatca ccatcttcag aacttccgaa attggctgat tccagccagg ccaggaatcc
    34561 atggctttca ggagtagtca gtgctttggc ttcatcccaa ttctgctgct ccagcgcttg
    34621 tataaaatga tcggtggtct tttcaacctg tttgttggca gaacaggcac tcagggtcag
    34681 accaagggtc atcgccatta ataggtggtt gactttcata gtggttggtt tgagaactgt
    34741 tacgaaggta agctgaaaat gttactcctg atcctgcctg acagcctccg tattggtagc
    34801 cagaaaagga acaacttttt gcgtttccag tcgctcattg caaaagtgaa tatcgaccgg
    34861 agcatgatga aacacttctt ctgatagttg aatggtgaac agacctacgt ttcggagtgc
    34921 tgtactgtca agggtagttg tatcaggaaa tacccagcgg aacaggtaag ttgtctggtc
    34981 cttatcgagc tgtacggtga tgacctggcc actgaaaatg ccgtagtcca ccaggtattc
    35041 gcccagtctg gtagcctctg attctgtaac ccgctccgtg aagtacagtt cggcattatt
    35101 gaaatgcagc agggcaccat agtctggcct ggaacaggag cccagaacaa gcatcatgaa
    35161 caggagcaag ggatagcgca ccttcaaatt taccatatag ctgagattca tgcacaaaaa
    35221 aaagccggtc atctggtgac cggcttattg atcgtgttca ttatcggatc agctgcaacc
    35281 caggctttct ccgcagttca ggcacttgta gcaggtaccg ttccggatgg tggtatggcc
    35341 gcacttgtga cacggaggtg catcgcccat cagggtcttg ttggcagcag ataccggatc
    35401 gccgtttgcg cctgttgctt ttttctccac taccactgct accggttcca cttttttagc
    35461 gtgtggtttt tctaccggat actcgcctcg tgcttctgat gacagatcca gcggagactt
    35521 ctcttctatc tcttccggtg ccacgtgaac caggtctgtc cggtccaggt attcgaatgc
    35581 caggaggcgg aaaacataat ccactatgga agtactgaac ttgatgttcg ggtgatctac
    35641 cggaccactg ggctcaaagc gggtgaaggt gaacttgtct gcaaattctt ccagcggcac
    35701 accatactga agcccgatgg agacggcaat ggcaaaacag ttgaccagcg aacggaaaga
    35761 tgctccttcc ttatgcaggt caataaagat ctcgccgagt gtattgtcat catactcgcc
    35821 ggtacgaacg aatacggtct gtccgcctat cttggctttc tgggtgaaac cactgcgctt
    35881 ggcaggcagc ttcttccttt ccaccaccct ggacagctga cgcttgaaca tggtatcctt
    35941 agaactcatg atgagcgagc gggctgcttc cagcacttcg tcttcggtcc actgatggtc
    36001 tgtactgacc tcgtctttct tatcactttt ggttgacaat ggctgagaca gtttgctgcc
    36061 atcgcgatac agggcattgg ctttcaggcc gagttcccag ctgagacggt aacattcctg
    36121 gatctcttct accactgctt catgcggcag gttgatggtc ttggaaatag cgcctgaaat
    36181 gaacggctga acggctccca tcatccgtat atggcctgta gccggaatga atcgcttacc
    36241 gatcttacca ttcttgttgg cacagtcaaa tacctcatag tgtttctcct gcaattgtgg
    36301 tgcaccttca atggtcatgg taccacagat gacatcattt gcctcatcga tctgagcctg
    36361 gctgaagcca agtgctttca acaggttgaa catggggtcc atgtattgtt cactcttgaa
    36421 gcccattcgc tgaagtgcca cttcgccaac tgtgaacacg ttaaatgcga aagtgatctc
    36481 aaaggcagac tgtaccactt tgtccagatg gtgcagttca ctgtcggtaa aaccttttgc
    36541 tgcaagcgat tcaaaattga tatggggtgc gccctgcagg gttccggttc cttttgcgta
    36601 atccacaatg gctttgatgg aatcttcctt gtagcccagg tttcgcagcg ccagcgggat
    36661 agactggttg atgatcttga agtatcctcc accagacagt ttcttgaatt tcaccagtgc
    36721 gaaatctggt tctactccgg tggtatcaca gtccatcacc aggccaatgg tgccggtagg
    36781 tgcaatgacc gttgcctgtg cattccggta gccgtgttcc tcacctagtc tgacagcatc
    36841 atcccatgcg ttacaagctg cacgaagcag atactccgga cacagcttct ggtcgatgcc
    36901 cattggtttg atctccagtc cttcaaaatt atctgcagca ttataggctg cataccggtg
    36961 attgcgcatt acacgcaaca tgtgttcctt gtttcttgca taaccggcaa acggccctat
    37021 ggcggctgcc atctctgctg aagtcttata ggagataccg gtcatgattg cagatatgga
    37081 acctgcaatg gcacgggcct tgtcactgtc ataggctata ccacttacca taagcagaga
    37141 acccaggttg gcaaagccaa gacccagtgt gcggaattcg taagacagac gtgccacttc
    37201 ctcacttggg aactgcgcca tcagcacaga tatttccagc acaacggtcc aaacacgaca
    37261 ggcattttca aacgcagcta catcaaaaac gagcttctca tcatcataga acttgcgcag
    37321 gttgatagat gccaggttac aggcggtgtt gtccaggaac atatattctg aacaggggtt
    37381 ggaggcattg atgcgaccat cggcaggaca ggtatgccat tcattgatgg tgctgtcaaa
    37441 ctgtacaccg gggtcagcac aacgccatgc tgcatgggct atcttatccc acaacttctg
    37501 ggcattgacc ttcttcatgg tctcgccgtt gctgcgggct ttcagctccc agtcgccatc
    37561 ttcttccagt gctttgaaaa agctgtttgg aatgcggatg gagttgttgg agttctgccc
    37621 ggctacggtg cgatatgctt caccttcata atcgctcgga taaccggctg caattaaagc
    37681 ggctactttg tcttcttcct taaccttcca gtcaatgaag ttttcaatct ccggatggtc
    37741 gagatccagg cataccattt tggcagcacg gcgggtagtg cctccgcttt ttattgctcc
    37801 tgcagctgca tcaccgatct tcaggaaaga catcagcccg gaagatgttc ctccacctga
    37861 aagtctttct ccttctccgc gaatgcggga atagttggtg cccactccgg agccatattt
    37921 gaagatacgg gcctcacggg tccaaaggtc catgatacca ccaggattca ccaggtcatc
    37981 atctacactc agaatgaaac aggcgtgagg ctgcggacgc tcataagcag acgtggattt
    38041 tttcagttta cctgaatcag gatctacata ataatgaccc tgtggtttac cactgatacc
    38101 atatgcggta aataagccgg tgttgaacca ttgcggcgaa ttgggcgctg catactgccc
    38161 gatgatcata taaaccagct catcataaaa gatctgtgcg tccttggaag aggcgaagta
    38221 accgtacttc tctccccatt gtttccagca gtcggccagg cgatgagcca cctgcttgat
    38281 agaggtttcg ctgccggtgc tgccgtcttt ctgcggaaca cctgctttac gaaaatattt
    38341 ttgtgcaagg atatcagtgg ctacctggct ccaggagttg ggaacctcca catggttcat
    38401 ctcaaaaaca atgtcgccgg tggtgttgcg gatcacgctc gagcgattgt catactcgaa
    38461 catgtcaaac gggctgatgt cgtcacgggt aaaattgcgc tgaaaggaaa gtccgttcga
    38521 tttcgccttg tgtgctgcca tagtctgttt cagttgaata ggttaaaaaa gggtcgtttt
    38581 caggttcagg agaaaacaaa ccacccgtct ttttgaccgg gtggtgcacc gaaagtacga
    38641 agcatcagtg tagcctttca agtaacattt cttcacaccc ctgtgaataa acggcggagt
    38701 gtttggtaaa cactgcattc agcctgtaca catttatatt ttaccaaacc gacacattcc
    38761 gttcatatgt tcttaacccg accgccgctc agtgaccgta ggggccgatg ccattttcag
    38821 agcacaccgg acacggccgg caaaaatgcc taattttgtc atctcacagg ctatgcacag
    38881 cattcgctcc tatatagaag acgcccgttc aagacaccag aaactatttg cggttctggt
    38941 ggatcccgac gatgtggatg agagttcact ggatcgaatt gcccggcagg cccttgacaa
    39001 tggagtggat ctattcctgg ttggcggcag cctggtaacc ggaacaaaac tgcctgatac
    39061 catcgcccgc ctgaaaacca ccaccaacct gcctgtggtc atttttccgg gcagcgtaca
    39121 acaagtggat ccgcaggctg atgccctgct tttcctgtct ctcatctccg gccgcaaccc
    39181 tgatctgctt atcggcaatc aggtgctggc agccccctat gtgaaacgct cgggtattga
    39241 agctattgct acaggataca tgctggtgga tggtggtgcc cctaccactg tttcctatat
    39301 cagcaatacc ctgcctattc cggctgataa aaatgacatc gctgtttgca ctgcattagc
    39361 tggtgaaatg ctgggcatgc ataccattta tatggacgcc ggcagtggtg cccgccaggc
    39421 tattccggca tctatgatca gggccgtcag tactcaggtg gatctgcctg tcatagtagg
    39481 tggtggcatc cgcacacccg accaggccta cctcagtgct gaagccggtg ccaccgtcat
    39541 tgtggtgggc aatgccatag aagaagacag taacctgatc agtgaactgt cttttgctgt
    39601 acactctgcc tccggacagg gggtcaaacg atgagcctta attttacagc atgcgtggac
    39661 gtgtgatcca gagtaccggc agctggtatg aagtgctgct ggacaatggt gccagtatca
    39721 gttgccgcct gaaaggccgg tttcgcctcg aagacaagaa gctgaccaac cccgttgctg
    39781 tgggtgatgt ggtggaggtg gatgctgatc aggaaggatc ttcggtcatc acagagatcg
    39841 gtaagcgaca aaattatctg gtacgggaat ctccccagaa aaaagaacat acgcacatca
    39901 tcgcctccaa cattgatcag gtggtggtga tcgcaacgct tcaccagcca cgcaccagca
    39961 ccggattcat tgaccgggtc ctgttcatgg ctgaggtcta tggtattccg gggtatgtgg
    40021 tgttcaataa gtgcgacctc tacgatgaag aagaaatgga tcagctcatg gaatacggag
    40081 gcatgtacct ggaagcaggt tatccggctt ttcatacttc tgcaactaca ggcgaacatc
    40141 tcgctaaact gaagtcatta ctgcagaata agacaactct tctgaccgga cattccggag
    40201 taggcaaatc ttctctgatc aatgcccttc aacccgaact gaatgtgcgg gtgggcgata
    40261 tcagtgccta tcatgagaag ggacaacata ccaccacctt cgcccggatg tttccgcttg
    40321 cctttggtgg ctttattgtg gacactcccg gtatcaaaga gttcggcatg gtgcacgtga
    40381 cagctgaaga agcaggtcat tatttccctg agatcagaga acgcatggaa cattgtaaat
    40441 tcaataactg tgtgcactat aatgaaaagg aatgcgccat ccgcgaagcg gtggagaccg
    40501 gacacatgag cgggcgtcga tacctctcct acctgagcat ctatgaagaa ctgcaagcaa
    40561 aaaaaccctg gtagatgcgg gcagtgatac aacgggtcag taaggcatcc gtaacagtgg
    40621 atggggaagt catctcccgt attggtaccg gtctgctggt cctgctgggt gtggaggatg
    40681 cggatacacc cgaagacgcc gactggctgg tgcgcaagat cattcagctg cgcatcttcg
    40741 ccgatgaaca gggtctgatg aacaagagta tccaggatgt tggcggagat ctgctggtgg
    40801 tcagtcagtt caccttgttc gcctcgacca aaaaaggcaa ccgcccgagt ttcatccgct
    40861 ccgcccgacc cgatcatgcc atacctttgt acgaagagtt cgtgcagctg gccgaaagcc
    40921 tgctgggcag acaggtggcc accggatctt tcggcgccat gatggaagtg gacctggtga
    40981 acagcggacc ggttaccatc accatcgata caaagaacaa agagtgaaaa gaatcaaagc
    41041 cttttttcag agatacaccg agatcatcct ggtcatcctg accatcgtgt tcgtgttcta
    41101 taaaagcagt ttgcctaaat acggcgaacg catcagtctg atgttcttcg gcaccctggc
    41161 attttattac ctggccagtg gtgtactggt gttcctggac aggcatcgca tcctgcgcac
    41221 catccgtctc atttatctta ttggcctgtg ggcagtcagt atggctgtga tcggcatcat
    41281 ggcccgcatc cacctcatca gaatgaatga ggaattatta tatatctcct gtgcagcact
    41341 ggcaggtacc ctggcctttt cctggctggc ttaccgtcag gtagatgaag gagagcagca
    41401 acaagcatgg aggtggcagc tgcaacccat tgtgatacgc agtaccgcag ccctgctctt
    41461 ttcattgggg atcgtgctca tcacccctta taccgtctat cacacttttg gcacctatcg
    41521 caatgatgcg cagtacacag aagatgccgt gtatgtatat gaacatccgg aagatactgc
    41581 tgcgcagagg aaactggaag aagcgacgtt gttaaaatcg gaggacggaa agcggaatgc
    41641 ggaataaggg gtggcactct tgcctgccag caggcaggga ttgaacagat ggccagaata
    41701 ttgtgttgaa atcgtcagtt aaaacaatcg tcttcactgt tacttaataa aattctgtga
    41761 gccacagagt ttgatatgat ccattttgca caataagtgc ccgctccgtc agagaaccat
    41821 ccgaatattt ctaaaatatc atcacttttc tcaagcagaa aatgtttgtt tgaaattcca
    41881 tcgtaaaaag ttggattgta cattctgctc acatccaggg atatagtgtc atcttcatct
    41941 atgaatatca ggcttttcaa ttggtacttt ggcaattcca tccctctgtc cataccaaac
    42001 cattcttcac catcaatgcg acatatatac ggggtttcat ttgggctaca agtatcatag
    42061 taatggtcta aataattgaa ttcatcgatc tccactctca gtatgatccc atcatcaaag
    42121 tggagttcac tttgtgcaaa agtgtggcaa gacaaacaaa cgcagaagaa agaaaataag
    42181 aatctcatga ctctgtttgc taaattgttg tacagatgtt gaagatacta atacctgata
    42241 cccctgtccc aatactcaat acccactacc cactaccacc tacttcatcc tcacaaaccc
    42301 ttcgccatca tacaccggta ccacgtcgta cttgtggttg cccatgcaga acttgatgtc
    42361 cttttcgatg ccgagtttgg cgaggcgttt gaagtgctgg gcctgcttca cagaacggag
    42421 catgttggcc cggatgtagc ggtacatcat cttggtgctg agcgtggcat cgtcgaggat
    42481 ctggaactga tcactcaggg cccttaccac agaacctgcg tagagcgcat cctccatatt
    42541 gtagtgatcc ttccaccctg cacagaacag gatggtatcg cgattctcct ccctgatcca
    42601 gtcaacaact gcctcgatat tcagaaagga acctgcaacg atgtttctgg caccctttga
    42661 catgagtaca caacgggtac cgttggttgt tgtgtggatg atggtcttgc ccttcacatc
    42721 atcgcccatg aattcgtatg gtgaattgcc gaactgaaaa tgctcgatct tatacccgtc
    42781 ccgttcaccg gctacgaggt aacctttgga cagatatgct tctgcttcct gcacggtctc
    42841 tacggggatc atcttttcga cgccgtggta cagggctgct gtgatggtac tggtggcccg
    42901 gatgatgtca atgaccacca cattcttctc cgcataatcg aactgatacc acaacgcagg
    42961 cgtcaggcat acctcgatca cggggtcaga atctactggt cttttgatga tggacatgct
    43021 gtcttactga aggaacggaa gcttgaccac cacagcctgt acgggcttct ggcggatgtc
    43081 aatatacact gttgagccgg gctctgcaaa agcagtgtcc acataaccca ttccgatggc
    43141 cttgcccagt gtgggcgatt gtgtaccgct ggtcacctta ccgatgacat ttcctgcttc
    43201 atccacgatg ggataatcat gtctcggtat gccccgatcc accatttcaa agcctaccag
    43261 tttcttcttc agaccatctg cacgttgctg cacgaaatga gccttattgg tgaattcgtt
    43321 gttcgctgct ttcagtttgg tgatccatcc cagtcctgct tccagcggac tggtggtatc
    43381 atcgatatcg ttgccgtaga gacagaatcc catttccagt cgcagggtat cacgtgcacc
    43441 cagtccgatc ggcttgatgc caaaggcagc gcctgcttcg aacactgcat tccagatcac
    43501 ttcactgtgc tctttgggaa agtacagttc aaatccgccc gcaccagtgt agccggttgc
    43561 agaaatgatc acattgtcca cccctgcaaa acgatgcttg gtgaatgtat aatatgcgat
    43621 ctcagacagg ttcacatcgg tcagagactg caatgcttct accgctttgg gtccctgtac
    43681 ggccaaaaga gaggtctgat cgctgatgtt gtgcatctcc acaccgcctg tattatgctg
    43741 actgatccag ttccagtctt tctccatatt ggaggcattc accaccagca tgtagttgcc
    43801 gtcttccagg tgatagacca gcaagtcatc cacgatccct ccatcattat tcggcagaca
    43861 ggaatactgt gccttacccg gactcagcgc atacacatcg ttgctggtga cccgttgtac
    43921 cagatctgca gctccttcgc ctttcaggat gaactcaccc atatggctca catcaaatac
    43981 acctacggca ttccgcacgg tctggtgttc atcattgata ccactgtagg agatgggcat
    44041 attataccct gcaaactcag ccattttggc tccgagttgc tcgtggatat gggtcagtgc
    44101 ggttgatttc atacgggttt tcgttgcggc gcaaaactaa gcaatttcac ccgaagcccg
    44161 tccgctgcgt acctttgaca caaagttcgc tgtatgcgtt ttacagaatc ctatacacta
    44221 gccgacattg cccagtggct gggggtgtct tatgccggta gcggcgatgc ccctgtaact
    44281 ggtctcaatg agattcacaa ggtggaagca ggtgatatca ctttcgtaga ttttcacaag
    44341 tattacgatg cggccttgaa cagtgctgcc accttcatca tcatcaacaa ggaagtggac
    44401 tgccctgcgg ggaagggact tctgttcagc gatgatccgt tccgcgattt tgtaaaactg
    44461 gcgaagcgct ttcgtcctgc agaacacagc ctgcatccgg tggccgatag tgccgttgtt
    44521 ggtgaaggaa ccatcctgta cccgggtgtg tatgtgggtc cgcatgtgca gataggaaag
    44581 aactgtatca tccatccgaa tgtggtgctg tatgaccaca ccatcatcgg caataatgtc
    44641 atcatccatg caggtgccgt gctgggtgcc gatgcgttct attacaagaa gcgcaaggag
    44701 cctcagccat ttttcgataa actatacagt tgcggacgtg tagtggtaga agacaacgtg
    44761 gagatcggtg ccgcctgtac catagacaag ggcgttagtg gtgatacggt gatcggcaaa
    44821 ggcagcaagc tcgataacca tattcacatc ggtcacggcg tggtgctggg cgaacgca
//
LOCUS       DAOMAX010000002        43114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_122_length_43114_cov_4.683783, whole genome shotgun sequence.
ACCESSION   DAOMAX010000002 DAOMAX010000000
VERSION     DAOMAX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 43114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43114
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_43114_cov_4.683783"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK742_00210"
     CDS             <1..538
                     /locus_tag="PK742_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQU75037.1"
                     /translation="PLVIVAAISYVTVKYFEPHPMPVRQLMDKGLFAGYDSDSRAISK
                     LDITKLIEQDFQTVQPNMNLGQLVEVVSHCSRNLFPVVDEEGAFRGIITLDDIRTLMF
                     KPERYADTYVSQLMHAPPTVLQKQEGMDPAMKKFDETGAWNLPVLDGDKYLGIISKSR
                     IFGVYRKLLSEESTRLEL"
     gene            652..3405
                     /locus_tag="PK742_00215"
     CDS             652..3405
                     /locus_tag="PK742_00215"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQU75038.1"
                     /translation="MKGKTTLLLFGLLLVTSLQAQVVINEFSAANYSDVADNYGDFED
                     WIELYNAGATAVDLGGYFLSDNASNPDKWEIPAGTTINAGGFLRFWCSGRDEVSGSNY
                     HTSFKITQTVVDEDVVFTAPDGVTILDVQYIEEPNQKNHSWGRYPNGSDNWYIYTDPS
                     PNASNGATHYGAYAIKPDMEPNSGNYTGSVTVSITSPQPDVTIRYTTNGSWPTAASPV
                     YTGPITLDETTVMRVVGFHADGDTLQSWCSTNTYFIDESTNVPVISISGEEVDDLLNG
                     NGWLHPIGSFELFDENFVLQDEAIGEFNEHGNDSWAYDQRGFDWIVRDQFGYDNDIDL
                     DFFSDVTDRTGYQRLIVKAAANDNYPESSGGAHVRDAYVHHLSQLGGLDLDERSTFFC
                     LVFLNGNYWGVYDCREKVDDNDYTDYYYKQDEYEMDFIKCWGGTWAEFGTMADWDPFV
                     NFVTTNDMTDPANYAYVTDNLNVLSLIDYIQVNTVSVCKDWLNWNTGWWRGYDPDGGA
                     LTWRYILWDNDATFGHYINYTGIPDDSPDADPCDPFGLYVDPNGHVDMVNALLENEDF
                     FSLYVNRFADMNATTYSCDMMLSVLDSMMNVIEPEMERHTDKWGGTYSGWMSNYEDLY
                     EFIEERCAYLEVEGMEDCFDTPAYPIEVQIDPPGSDNEVKVNTIIPADFPFSAVYFGG
                     VTFNLTALPASGYIFDHWEFLHNTPSSTTDEAITVGLESSDTIVAYFTADVLPTYNFT
                     LDIFPPDAGTATVIGVTPGTYPYSNSYLSGTIVDMQASANAGYVFDYWELDNHLVNPD
                     PFADDVFFIQSTVEDVLVHFRTTTAINDLVDGIAAFAVVPSLTQGNIQVQFEMTQAGE
                     VSIDLFTMTGSFAGNLSNGYQPAGEHSLSYNLQDMNLAPGMYFVRIQSNGISYNQQIV
                     YSR"
     gene            complement(3475..5100)
                     /locus_tag="PK742_00220"
     CDS             complement(3475..5100)
                     /locus_tag="PK742_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75039.1"
                     /translation="MKKVLLALPLCMSIVGLHAQDTYLQVYDIISAKCGSCHIEAHES
                     GLTLNGSAAEVYDAIYDAIPVNAEAAGKGMKVISPGDPYKSFLFSKINNGLALDVNLT
                     AGEGAACPQEGEPVLDNKQIELIRQWIIFGAKETGNMVDTTIIATFYDEGGIQSVPDP
                     PAPPAPEEGFQIHYGPWFLWPETEHEYWSKFATQIPEDLEVNRIDVIMGDYSHHFIIY
                     KYDAPTYLMNPYGLRDNDPEFLGVSLVTANQFSYDLHLPPNTAFPWESDTWLDLNSHY
                     INYSSTMPLACEVYMNVYTQPAGTAVQEMHTELPANTDIYIPNNGLEYTFEESLYDSG
                     DDEIFLWGISSHTHKYGTDYNVYKRKADGTKGEQIFDASCESTDGIPGCIDEIYDYQH
                     PPIRYWDSFLPIKVSEGVIHEASYINDGPSPVWFGLTSDDEMMVLIYFYLEDTAGLNL
                     PSSVPEQEPLIGIQSFPNPANEQVLVRSEEVFGRDLMLQLTDISGQVVLTQQVYAGDR
                     LAVIDRKQWASGLYLLQLTDNGTVVYSEKIFFR"
     gene            complement(5173..5700)
                     /locus_tag="PK742_00225"
     CDS             complement(5173..5700)
                     /locus_tag="PK742_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQU75040.1"
                     /translation="MALLLPVEGKNPVFGERCFLAPNATIVGDVVTGDDCSVWFNAVV
                     RGDVNSIRIGNKVNIQDNAVIHCTFQTAPTTIGNEVSIGHNAIVHGCTISDRVLVGMG
                     AIVMDHAVVHSEVLIAAGAVVLEHTILESGFVYAGVPAKKVKELTPEVFRHNIERIAN
                     NYTRYADWFRDDKIS"
     gene            complement(5678..6466)
                     /locus_tag="PK742_00230"
     CDS             complement(5678..6466)
                     /locus_tag="PK742_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQU75041.1"
                     /translation="MVYQTGLVLSGGGTRGIAHIGVLKALEEAAVEPEVISGSSAGAI
                     VGVLHAAGLSADAIFSLIRNKSLYSIVKMGLPNKGMMELSYFRDVLYKHLPGDDFSKL
                     RKPFYVAVTNLNKGGCEIIHQGDKLVDHVIASASIPMVFKPVQINGHLYVDGGVINNL
                     PVEPLRDKCRILIGVNVNTVEYIDQVNGIRDVGVRCLDISLQERVQNRLKRCDIAIEP
                     LVSRFGIFDLSRADDIYQTGYEAAKTAIFAFMAKQKTHGSAAAG"
     gene            complement(6510..6848)
                     /gene="gldC"
                     /locus_tag="PK742_00235"
     CDS             complement(6510..6848)
                     /gene="gldC"
                     /locus_tag="PK742_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HQU75042.1"
                     /translation="MSKESEIRIRIQLDDDQLPEQITWQASDSTAAEPQVSKAFLISL
                     WDPAYKETLRIDLWTKDMQLEEMNVFFFQTLVTMADTYLKANNDEKLAAEIRDFALRF
                     GEKTSVIKKK"
     gene            6957..7280
                     /locus_tag="PK742_00240"
     CDS             6957..7280
                     /locus_tag="PK742_00240"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HQU75043.1"
                     /translation="MKVYHRLMIFSVFILTGLVAKSQVVYVTEWKSDADKIVYVSEYT
                     SEADVVVFRTEWKSDAKPMSGIWYFTEWKSDADLRIYFTEWRSEADLVIAYTDWKSDA
                     VWRKK"
     gene            7283..8029
                     /locus_tag="PK742_00245"
     CDS             7283..8029
                     /locus_tag="PK742_00245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQU75044.1"
                     /translation="MLHLVADLGNTTGKFALLKDGELLSMHRTADRGTDALRTFLADS
                     VPDAWIISSVRTDSSEITAYLQDLAPGFVLDHQMPLPINITYETPETLGHDRIAAMVG
                     AWKMFPGKPVLVIDAGTCIKYDILLPDGSFPGGTIAPGLRMRLEAMHTFTGRLPFVEI
                     PEQTWPEGPPVTGTSTAGSLLSGALAAGLLEAEGMIAYYQQNQPYLKVLLTGGDAPFF
                     ASRLKSQIFAVPELILSGLDEILRFQFNAI"
     gene            8029..9390
                     /locus_tag="PK742_00250"
     CDS             8029..9390
                     /locus_tag="PK742_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75045.1"
                     /translation="MHFSRSLFLAFALACTAPAFAQLNSPYSRIGLGNIYTPTMGGSM
                     GLAGATGANLLTTEISAQNPATYCYLFKSVLDVGISGRFLTLDDGTETFTSGEGNLSH
                     LAFGFSPDNNFSKHDWGFSTGILPVSTSQYNIEQTTASADTLLGDLMSQYTGTGGLFK
                     YFVGLSYGLEFNKDTLVTLNKKGEADSSRFYRNEFSIGMNYEYLFGSIYNTTIASFPD
                     QVNSVDTKYQRQTSMQGGGIRAGITYQRRVGRVNNLNAGASFAPGLDLRGTQSVSWFN
                     INQFGTIQQITDTLYYAPDVAGTITTAPELNIGLAFNNYRQRSIDSVKFHVTAEFSYQ
                     DWSKYAGFLYSDSLASMYRIKVGAEVIPPRRDEVGKSLIPIIYRAGFYYGTSYLVIDG
                     QQLTEFGMTFGTGIPMRGSRVNLAVGFSQRGAAGLIRENYVNLQAGFNISDYNWFIKR
                     KQN"
     gene            9416..10774
                     /locus_tag="PK742_00255"
     CDS             9416..10774
                     /locus_tag="PK742_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75046.1"
                     /translation="MKSWLMLFLGAAALQAAAGEDDLSWSLQDTCRAEAENQEKFLEE
                     YSTYREFFKQENYKDALIHWRYVIKEAPGFRKQPFIDGISMYETFVEAEQDSARRELL
                     IDTVFMLYDKRIQCHDEAGFVLGRKGYSMYKLRSKDYGTIYETLKESFSLQGNKAEYF
                     LIPTYFNYTIIQWKLKKLQNDDVLNTYDELVGIIEYNLANNADEAEYYAKMRDAIDQM
                     LPANLLSCEALIERVNSRWEQDKDDVNKITRYYYSMRIARDSSGNSCLETKEYEMVVR
                     RLYELAPSSELAEQVAVYLFKEGKENEAIEKFNEAIEMETDADKKGSLCLNIARILRS
                     QNKFSQSRTYAYKALDYKPSWGEPYLLIGDLYASSGPLCGPRTGFDSQVVVWVALDKY
                     YKAKSVDPSVADDANDKIARYSQYMPTKTDLFERGIDEGSSYTVGCWINESTTVRGRK
                     SN"
     gene            10837..11397
                     /gene="lptC"
                     /locus_tag="PK742_00260"
     CDS             10837..11397
                     /gene="lptC"
                     /locus_tag="PK742_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQU75047.1"
                     /translation="MFGRLYFICCVLLLAGCVNDMSEVEQTAALADPGIEKGKDVVLY
                     YSEDGMVKMCIQATTLWRYLTDDPYVEFKDGMKVEFFNDEEKVISTLTSGYGIRYEKQ
                     LKTIVRDQVVVINENQEKLETEELVWDEKKHIIFTDKYVSITTADEVLYGTGLEADET
                     MTEYTILHPEGIIKIDMDDAQTDEDL"
     gene            11384..11614
                     /locus_tag="PK742_00265"
     CDS             11384..11614
                     /locus_tag="PK742_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75048.1"
                     /translation="MKIFEVLWLAASLIALTMAIIRATSGQAYGNYIYITLFTAAVAL
                     FMYRFKKRNRLYLEEREKEHIRQQEELQKKHE"
     gene            11607..12431
                     /locus_tag="PK742_00270"
     CDS             11607..12431
                     /locus_tag="PK742_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HQU75049.1"
                     /translation="MNNLTQRILTGVVGAAVIIFLLCWHAWSLTILFLLLAMFTQFEF
                     LRVVNKLKEKADSPAHIYGNLFAGLLNFLLITGSMAGWLPGMAWTGTLPVLAAIFLLE
                     LYQDRPNPFQHIGFRLTGQIYIILPFAWMAVLGTAGNSFQYLYPMGVLFIIWTNDVFA
                     YFTGRLLGRNRLFPRISPAKTWEGFIGGVIMALVCASIFDHFDQNQPLFVWLVLGFLL
                     AVVGTGGDLVESLLKRNLQMKDSGSILPGHGGFLDRFDALLLGAPVAFAWLAIWIF"
     gene            12475..13152
                     /locus_tag="PK742_00275"
     CDS             12475..13152
                     /locus_tag="PK742_00275"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HQU75050.1"
                     /translation="MFRIIHKEGRTILAIFGLLLVALNIVLQRMFPEDLSLHIIALVV
                     SLGALFFVLYFFRNPKRLLFIEEGAIIAPADGKIVVVEETDEPEYFGDRRIQVSIFMS
                     VKNVHVNRTPIGGQVKYFRYHPGKYLVAWHPKSSTENERTTLVIEDNDDQVEILMRQI
                     AGTVARRIKFYVEEGEDVSQGAEFGFIKFGSRVDLFLPLHAIVEVTPGQKVKGGQTVI
                     ARIPGKH"
     gene            13175..13501
                     /locus_tag="PK742_00280"
     CDS             13175..13501
                     /locus_tag="PK742_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75051.1"
                     /translation="MKRFFLVIAILGAGVIAAQAQSNTSVAPASDATPVVNAADHMGQ
                     GKSCCSAAGQEKQSCTAEKAASAAKDQKKGGSCCSAGGNAEAQSGKSCEGVSFGMGAA
                     KPSKDE"
     gene            complement(13504..14208)
                     /locus_tag="PK742_00285"
     CDS             complement(13504..14208)
                     /locus_tag="PK742_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HQU75052.1"
                     /translation="MHNFSHIRHIFFDLDNTLWDFSANSHRILYEMYQAFDLQEMGIH
                     AFDSFHDQYRMRNSHLWSEYKLGNKTREEVRLERFQHTLYDHGIDNRLMAMRLADYYI
                     ENTRRVKDLVPGAVELLEELSAKYPLHIITNGFEEVQDFKLVNTGLRKYFRSITTAES
                     AGQLKPGKKIFEHAMRQAGAEASESLYIGDSPDADGHGSMNAGMSFIWFNYKKEQNTH
                     RFNCAVTELKAIGAML"
     gene            complement(14201..15217)
                     /locus_tag="PK742_00290"
     CDS             complement(14201..15217)
                     /locus_tag="PK742_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQU75053.1"
                     /translation="MSRILVTGATGFTGSYMVRYLRQLGHEVRILHRSESSWYLLEEV
                     EGLEKFCGDIRDIFALSDATEGVDAVYHCAGLVSYKKEDKNRLHQINAAGTEAVVNAC
                     LDRNIPMLHLSSVAAIGKKYTRGEVITEQNKWESRKALSDYAVSKFRAEQEVWRGVAE
                     GLQAVIVNPSIILGACDWEQSSGRLFRSVYKRYPFYTQGITGFVDVRDVVQMAHQLME
                     EKHFGERFLLSADNLTYQHLFETMAKYLQVPPPRWEAGPKLSGLAWRLNAILRMFGLG
                     NKAITRTTARMANRQTRYDSSKVKSRLGMEFRSLEETIRDSCVCFQATQQDGCHHPLS
                     FEHA"
     gene            complement(15214..16290)
                     /locus_tag="PK742_00295"
     CDS             complement(15214..16290)
                     /locus_tag="PK742_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQU75054.1"
                     /translation="MTNNKVRVLGSMSGSSLDGLDIAVCTITRTDWLYSIDAAETIPF
                     PAPLRQLLKTYSGSTDRNDTLEVSAQLAAFMGAEIQAFLSRHQLDNPDLIASHGHTLS
                     HHPEAGHTLQIGNGQILADLTGLPVIWDFRMADIAAGGQGAPLVPICDELLFPQYQLC
                     LNIGGIANVSMELDGRRIGFDICGANQLLNALAEEAGREFDERGSMAASGRVAESLLH
                     QLNADDFFCLPPPRSLDNAYVQQNWVRVLKADDHSLPDRLRTATEHIALQIAQAMDLH
                     QKNGRVLLTGGGAYNDLLTERIRALSRHHVSLPDDHIIRYKEALAMALMGALRWWEMP
                     NFLPSVTGARSAVSGGVISYPTEQ"
     gene            complement(16280..16624)
                     /locus_tag="PK742_00300"
     CDS             complement(16280..16624)
                     /locus_tag="PK742_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQU75055.1"
                     /translation="MARLPVLFFVTAFLISACNNSNGNRPSGNQQAAQAATNELPGQA
                     VYQKNCKICHGSKGDLGLSGAANLRKSTLTPDEKIGVITFGRNGMAPHKDILTAEEID
                     QVAAYIETLHDE"
     gene            complement(16668..18806)
                     /locus_tag="PK742_00305"
     CDS             complement(16668..18806)
                     /locus_tag="PK742_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75056.1"
                     /translation="MNALRYTLLSLVILLVSHTTYATHIIGGELYYTCTGPNTYDVTL
                     VLYRDCSPIGGGTPFDDPAYLGIYDEDGTLLNTVSMFSPVLSDVPIEVDNPCLIVPPG
                     LCVEQAIYTVSITVPDDLQVFDLVYQRCCRNASIVNIVGPEDTGSTMWQQIPPGEDAS
                     CNSSPVFTNYPPSVICVGDPISFDHSATDPDGDSLAYKFFTPYAGASPFDPAPFPPPP
                     PPYDPVTWSAGYDVLYMVDGAPGFEIDVNTGLLTGTATDEGRYVLGVVVEEWRDGVLL
                     GNHIRDFQFNVQLCEPALVSTLGVDGEIVTDVYLDCEDFTIPFSNFSTGADSYLWLFG
                     DGATSEDYEPVHVYGDTGVYTVSLICNPGFVCADTSVVEIHIFNTLTADFEFNAGCSG
                     EAVQFSDASTSTEAGEIISWEWDFGDGGSSTAENPSHDYTIGGIYEVALTVTTDKGCS
                     STVLLDVPLLSGPTADFSADDVCLNEDALFEDETTHPDGVTLTDWFWDFGDGGTSGDQ
                     DPSYSYEEPGTYEVTLIVATNNGCQDTITQSITIGELPYPDAGLDDTVTYLEYYTLMG
                     EGNGTFSWSPAAFVSNPFIANPQIRPDQTYDYILTVTSPDGCSASDTVTIYVEDITIV
                     DVPNAFSPNDDGVNDLFFVYTHTVADFYEFSIYNRWGQQLFTTNDPTFGWDGKYNDEP
                     QDVGAYVYVVRAKDITGDLINLQGSFILVR"
     gene            complement(18839..21172)
                     /locus_tag="PK742_00310"
     CDS             complement(18839..21172)
                     /locus_tag="PK742_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75057.1"
                     /translation="MRSWLSILLCFGLALPAKAYHIVGGEMYYDCLGGNEYLITLKLY
                     RDCSGDGAPFDNPLYMAVYNGDGTLFQSLELPFPGSTLIDPDLTNPCMVNPPDVCVEE
                     AIYTRTITLPDNPLGYDIVYQRCCRNSTIVNLIFPEQQGATYTAHIPDPGSICNSSPR
                     FNSFPPVVICAGYPFNFDHAASDPDGDQLVYEFCRPYQGASFDVPAPEIPSPPPFPYV
                     TYTGGYSEGYPLDASPPLVIDSLTGWMEGTPASIGQYVVGVCVKEYRDGVLIGEHYRD
                     FQFNVTDCTPGLYADFPDEYNNCDDYTIFFDNNSFGATTWFWDFGDPSVDNDTSVVMS
                     PSYVYPDTGTYTVMLVAKPYTVCADTAYSIVQIYPGLVANFAYEPQCAGQPLNFSDVS
                     VSTYGYLEQWSWSFGDGTEGTGNTPSHVYEEGGTYEVHLQILNGYGCTKGVEHTVTVW
                     PTPDVDFVWDDACLQSVGTLTEQVFIDSAYTITGLEWTYPDGTGSTGSFGAYYFDTAG
                     AYPVTLVATSNKGCMDSITNQVVVYPKVVADVFRDSTICEGDTIQLFTRNGKYYQWSP
                     NYNISDLLDHDPFVYPDVSTTYTVVISDDCTSDTAQIIIEVLPAPDAVAWNDTAVYRF
                     EPVMLHAAGGVSYSWDPPNYMNDPFSQDPVVMPSETDSYVVTVTAENGCTATDTVSIV
                     VWLRCMRFTIPTAFSPNGDGLNDVFRISSFGDDEVSNYQIYNRWGELVFEAGSIDTAW
                     DGTTDGKKQETGVYIYYITIACEGQQAVYSGNITLLN"
     gene            21410..21943
                     /locus_tag="PK742_00315"
     CDS             21410..21943
                     /locus_tag="PK742_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75058.1"
                     /translation="MQIFILVLAIIFTLIVVVGLFIPRKQTFTETKDIAADKRRIFNL
                     IDDLDSWETWSAWSSLNDTDLQKEFPGKHSGSGASFEWKGSKMGKGSVEIVRAEPYSS
                     LEIATQFRNKRFRVIHHIQLHADRLPVQLTWTATVKTGWSSPSRILGRAVMNNMQTDI
                     HKSLILLSQLAGTAAEA"
     gene            complement(21917..22939)
                     /locus_tag="PK742_00320"
     CDS             complement(21917..22939)
                     /locus_tag="PK742_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75059.1"
                     /translation="MTVARVNYINIALMLISAAVAFVIPFELFLFSYAVLGPLHYLTE
                     ISWLHKRQYFSPNKYDWILLTLLTFFLTLPGIMLHFGPKDINGEPLISKSLYTFFDLN
                     PAVMFLAFSASLIMILVKKLNYRIISFVLLAIVGYMIRNYDLSQVIFTIFLPTLIHVF
                     LFTGAFILVGALKSHSTSGYLSILVFIGCAVSFFFILPDGAGYQISEYAKRSYDVSFR
                     SLNAQIFQSFLHEPAAGEDAIYYSSAGILITRFIAYAYTYHYLNWFSKTSVIKWHEVS
                     KPQLVAIFALWIIAVVLYYTDYRTGLMALYFLSFLHVVLEFPLNFQSFRQIGQQIRLR
                     FSGSAG"
     gene            complement(23037..23549)
                     /locus_tag="PK742_00325"
     CDS             complement(23037..23549)
                     /locus_tag="PK742_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQU75060.1"
                     /translation="MLSKKMEEALNAQIVMEGYASSYYLSAASFCDHKGLAGIASFLY
                     AHADEERMHMMKMFHYVNDCGGHAQAPALEQPPLQFGSVLELFEAILAHEQKVTASIN
                     NLVDISLQEKDYGTNQFLQWFVMEQHEEETLFRGVIDKIKLLGNDPKANYYIDKELGG
                     MRTESAGGEA"
     gene            complement(23641..23805)
                     /locus_tag="PK742_00330"
     CDS             complement(23641..23805)
                     /locus_tag="PK742_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75061.1"
                     /translation="MKSAGMKTIGKPFLMLVILTTMLLVAGCNLGGDKCDCPKFGSVE
                     NSTMQTLTTP"
     gene            23847..24953
                     /locus_tag="PK742_00335"
     CDS             23847..24953
                     /locus_tag="PK742_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU75062.1"
                     /translation="MPAETSNKTVLVAPLDWGLGHATRCIPVIRELLSMGCHVVIVTS
                     GRPLLLLQKEFPEADFYDLPSYNIYYQKRGSFLVKLTIQLRKILGGIMREKRVTSQLI
                     EQYDPVMIISDNRYGVRSKHVHTVFISHQMMIKIPGSKFIEWIVWQWLNFQHRRFHRI
                     WIPDVAGEPNLSGDLSHLHPVLPNTTFIGLLNRFSGEYDTTLPPNDVLCILSGPEPQR
                     SIFEKMILEQATAIRRKFIIVQGLSEERSDQWVSDHVRLVAHAGARKLHQLVASSQII
                     ISRGGYTTLMDLAPLGKHCIFVPTPGQTEQEYLVEELARHDLCVYQEQKHFDLLSALQ
                     QAGSIRPFQLEVEANTFVPQLHAEWQAALQRKMM"
     gene            complement(24933..25760)
                     /gene="murI"
                     /locus_tag="PK742_00340"
     CDS             complement(24933..25760)
                     /gene="murI"
                     /locus_tag="PK742_00340"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQU75063.1"
                     /translation="MDLGATPIGIFDSGYGGLTVMRSIVDRLPQYDYVYFGDNARTPY
                     GNRSFETVYEFTRESVFHLFDMGCELVILACNTASAKALRTIQQQDLPRMASGKRVLG
                     VIRPTTELIGQLTHSGQVGVLATNGTVNSASYVIEAGRFFPHVEVHQQACPMWVPLVE
                     NNELDGAGTEYFVQRSVEQLMEQDPRIDAIILGCTHYPLLEPVIRRFLPPHIHLLSQG
                     PVVAERLDDYLERHPDMAGRCSKTGKRTFFTTDDPASFNRHATIFFGESVASHHFSL"
     gene            complement(26406..26930)
                     /locus_tag="PK742_00345"
     CDS             complement(26406..26930)
                     /locus_tag="PK742_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQU75064.1"
                     /translation="MKKSLLVLLVLLATAGSSFAQRFAYVNTDYILGNIPAFEEAQKQ
                     VDEISKQWSAEITKKQKEIEDLYRKFQNEQYLLTEEVKKQRIQEIEAKEKAMKDYQKE
                     KFGYEGELFQKREELVKPIQDEVYAAIEKLAKERGYDFIFDKANSTTVLFADEKNDRS
                     DDVLRILGVTPGGK"
     gene            complement(26942..29590)
                     /locus_tag="PK742_00350"
     CDS             complement(26942..29590)
                     /locus_tag="PK742_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQU75065.1"
                     /translation="MRLLRPITLMLLLLPGLSAMSQTNLGKDTTAIDFAQPKELEIAG
                     IRVEGIVTLDERSLVARSGLFVGQMINVPGDETSRAIQNLWKLRLFSNIRIEAEKIVG
                     NQVFLVIQLDELPRISKYSPDGLSKAEEDDIVEKIEIVRGTPYTDFVRKDIETKIKDY
                     FADKGFLSTTVEVYAKPDTSQMNWVVMYIDVVKGPKTRISDIVFYGNEEISDRKLRAT
                     MKDTRVKTQLRPKYQDPEAPEPNYNPVYILSNLSVNSIRDYVSDRASLRIFNSSRYMP
                     ENLSADKKQVIALYNAKGYRDARIVEDTVYYVDDRNLRIDLYVEEGNKYYFRNISWRG
                     NAKYSEGRLDSILGIKPGDIYNQQLLEQKLRYDPVNGDVTSLYMDDGYLFFQLNPVEV
                     AVVGDSIDLEIRIQEGAQATINNIIIKGNTKTKEHVVRRELRTYPGQKFSRSALIRSQ
                     SEIARLGYFDPEQIGINPVPNPADGTVDIEYTVVEKPSDQLELSAGYAGGVFGGVVLQ
                     AGIVFNNWSMSQVFKKEAWRPVPSGDGQTLSIRVNTNGRRYQSYNFAFVEPWLGGKKP
                     NSLSLSAYRSFFADNDDADFTTLEGSFTTNGASVGYGIRLKFPDDYFNLVSTFSYQLY
                     QLDQYEGFIIENGNSHNISIKETLSRSSVNNPQFPSSGSNITFSVQFTPPYSLLNEKN
                     YDGLENTEKYKFIEYHKWRFTADWYTPVVGKLVFRSAAKFGLMGYYNKDIGISPFERF
                     QLGGDGITNFNFYGTDIIALRGYEIFNVASGFSTARTEPIFTKYTLELRYPISLNPSA
                     TISAHAFAEAGNLWSSFNEFNPFDVKRSVGVGLRAWLPMFGLLGLDYGIRFDEKLPGE
                     LVPSTGVFNYIVNNGKFTVVLGFEPE"
     gene            complement(29587..30336)
                     /locus_tag="PK742_00355"
     CDS             complement(29587..30336)
                     /locus_tag="PK742_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQU75066.1"
                     /translation="MSEPAAEQHASIPRHIAIIMDGNGRWAKQHGRARIYGHHQGVES
                     VRKVVEACSELGVEYLTLYAFSTENWKRPIEEVNALMELLVRTIRKETPELNSQNVCI
                     RTIGNTGSLPEDCVNELQEAISLTAGNTGLNLVLALSYSGRSELAAAMQAMGKEVAEG
                     RLDPDTIDEQTIASHLYTVDIPDPELVIRTSGEYRISNYLLWQSAYAEFYFTETLWPD
                     FDKEELLLAIHSFQQRERRFGKISEQLPMKP"
     gene            complement(30364..31224)
                     /locus_tag="PK742_00360"
     CDS             complement(30364..31224)
                     /locus_tag="PK742_00360"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQU75067.1"
                     /translation="MKVLKAICITGLGWLLAMPLTASAQQHEIGFWGGMGHSFGDINN
                     SRKSIQFAEPAFGMFYRHNFNTRLAWYIGGAFGSTYGEDAISDDLFQQTRNLSYRTGL
                     WEVASRMELNFFSLSRTKKNEWFSPFVFAGLALYHFNPQAYYDGQWIDLQPLGTEGQN
                     AEELSGLDPYYRYQVAVPLGGGFKVALSKNFTFGVELNWHKLFTDYLDDVSSVYFDPA
                     LLAIQPNGDLAVALADRSAELSDIQPIGGGGRQRGDALHNDAYVYAGAFLSYSIVNLK
                     CPMPGGGKKF"
     gene            complement(31352..32161)
                     /locus_tag="PK742_00365"
     CDS             complement(31352..32161)
                     /locus_tag="PK742_00365"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQU75068.1"
                     /translation="MSQFCRAIGTSRQNVYQRLQKAEAEWQATQSLRYMVDAVRRDHP
                     RMGAKSLYTRLCPEHMGRDKFLIWYQENGYSVHPQKNWRRTTDSSGVVRFDNLINELQ
                     LTDVNQVWVSDITYYELGSRFYYLTLIMDLYSRVIKGYAASQTLKCEHTTIPALKMAM
                     RHVYKHQTPILHSDGGGQYYSKAFLTLTKGRFLNSMGKTAYENPHAERLNGTIKNNYI
                     KPYNPTSYGQLKSYLAKAVRMYNQEKPHQSLGKVTPFAFECQVKLQNKQLI"
     gene            complement(32158..32583)
                     /locus_tag="PK742_00370"
     CDS             complement(32158..32583)
                     /locus_tag="PK742_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQU75069.1"
                     /translation="MSQTKGTRPQWHRQQSRHFSESFKQDKVKEIECKLTTVNQVSRQ
                     HGVSRTAVYKWLDKYSVNNGRKARLIVEPMSDSSKIDALQARIRELERIIGQKQIELE
                     FKDKMIDLAEEQYQVDIKKKLGSKLSDGLSPDKTNTVGQ"
     gene            complement(32859..33740)
                     /locus_tag="PK742_00375"
     CDS             complement(32859..33740)
                     /locus_tag="PK742_00375"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HQU75070.1"
                     /translation="MKIAVYGRLIHKEAIPYVQELFDLMQQRGIDFHIYEAYYPHLIN
                     NIKFSEKPALFSASDQKALQDTDYMLSLGGDGTLLDTVTLIKDSNIPVLGINIGRLGF
                     LASISKDEIRACIDALERRSYTPDPRTLIHVDANVPLFDGHPYGLNEFTIHKKDSSAM
                     ITIHVYINGEFMMSYWADGLMVSTPTGSTGYSLSCGGPIIFPTSANFVITPIAPHNLN
                     IRPIVVGDDSVISFEIEGRSEKFLASLDSRHETIGSNVQLAVRKEHFEITLLRLHETN
                     FLDTLRNKLGLGYDKRN"
     gene            complement(33718..35157)
                     /locus_tag="PK742_00380"
     CDS             complement(33718..35157)
                     /locus_tag="PK742_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQU75071.1"
                     /translation="MPKWLFVTMICVLCLPLRGWSQNEDLLVVDTIVVSGNDHTKAYI
                     ITKQMLLHPGDTITASSVDSLLERSKENILNTGLFLKADCTLFNNTDRQATIQVQVDE
                     RWFLFPAPWIDIADRNINYWWFEQNHDLSRLEYGLDMVHYNLTGHADRLNVRAIFGFS
                     QKMELEYERPFLSKDSETGWGLFAMYAANRQIYVNTQYDQQVFMETEDFLRKRIKLGT
                     YVRHRQGPYELHTVEIKYNDTRVSGAMDSLNPEYLGNGAHRLQFMTLGYTFISNRVDN
                     RSYPLEGNYLRAILQKRGFGFFRSDDPDNNTADQIFVSGQWNIYRHLTGKWYAAAMLY
                     GSQNLSAEYPYFLTESLGYCENFVRGYEYYVVDGQGIGLFRSNLRVKAIDKTLHSPIF
                     RNSQFGTIPVQVYPKVFADAGYVSDDVFTSTNTLRNTLLYSAGIGMDITTYYDWVFRF
                     EGAVNALGEFGLYLHLGLDLNTYENCSLW"
     gene            complement(35159..35821)
                     /locus_tag="PK742_00385"
     CDS             complement(35159..35821)
                     /locus_tag="PK742_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQU75072.1"
                     /translation="MLAKDIITENIPPLKSSDTGEKAIDWMMEFKLSHLPLVDNRVYI
                     GLVSEEDILDFNDTTEKLGAYLKNLYKPYVKASEHIFEVLRVAAGLNSPVIPVVDEEM
                     HYLGMITLQSLLYHFAAMTSISGAGGVIVLELGNKKDYVLSDIARIVESNDAHILSSF
                     VHQHGEDSHTVTIKVDKTDIRHIIATFERFEYEVKAYFEETDLGEIYKDRFDSLMNYL
                     NI"
     gene            complement(35823..36581)
                     /locus_tag="PK742_00390"
     CDS             complement(35823..36581)
                     /locus_tag="PK742_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQU75073.1"
                     /translation="MEHTFTEENGFRYVEEGEGEVLMLLHGLFGALSNFYDIIEAFKD
                     SYKVVIPILPLYELPIEETTVRGFVQYVERFVAFRGYDKVHVLGNSLGGHIALLYALD
                     NKDKISSITLTGSSGLFESAMGDSYPKRGDREYIQNKAELTFYNPKHATEELVDELFD
                     IVNDRNKVIRVLATAKSAIRENLADEITSITAPTLLIWGNNDTITPPFVGEEFHKLIP
                     NSKLLFIDECGHAPMMEQPEQFNRYLREFLDQLK"
     gene            complement(36629..37192)
                     /locus_tag="PK742_00395"
     CDS             complement(36629..37192)
                     /locus_tag="PK742_00395"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQU75074.1"
                     /translation="MILLLDNYDSFTWNLYDHLCQTGADVTIHRNDAITLNEIGALQP
                     EAIVISPGPETPLQAGITMQVIDRFHASVPMLGVCLGYQAIGMYFGARLERSPVPVHG
                     KTSMISHSGQDLFAGLPDTMQVMRYHSLNIADVPAQLEITAVTDRAEPMALRHRELPL
                     WGVQFHPESILTPDGLTLLHNWRNLYR"
     gene            complement(37189..37746)
                     /locus_tag="PK742_00400"
     CDS             complement(37189..37746)
                     /locus_tag="PK742_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQU75075.1"
                     /translation="MILDVFYAGVLLVALFRGYSRGLIFSLFWLLSVLVGIAAAVSCS
                     HITATYLEKWFHLHADYMPFISFLVTMFLIGLVFRLLGRLVEGVFQALQLNFLNKLAG
                     AFVWMLSWTLVYSSILWYGNNMGVFTEELKTASKVYEPLVGFAPAAMDLIGHAVPLVR
                     DVFEHMQDWFDKQNGVLPVPETMEV"
     gene            complement(37743..38195)
                     /locus_tag="PK742_00405"
     CDS             complement(37743..38195)
                     /locus_tag="PK742_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQU75076.1"
                     /translation="MSLLDTVNKDIVQAMKSKEEATLRALRGIKSAILLAQTEKGASD
                     ELSAEKEMQLLTKLVKQRKESLDIYTAQNRADLAADEAAEIAVIERYLPAQMSEDELK
                     TALEKIIAETGASGPGDLGKVMGVATKSLAGKADGKAISAMVRNLLAG"
     gene            complement(38276..38722)
                     /locus_tag="PK742_00410"
     CDS             complement(38276..38722)
                     /locus_tag="PK742_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HQU75077.1"
                     /translation="MSITINTPALLFPAISLIMLAYTNRFLALSSVVRNLHEKYLRNE
                     TSPFLKEQINNLRFRLKLVRRMQFLGVLSFILALLSMYFIYIEYMSWANACFAAAMIL
                     FSASLVISLIEILRSTRALELELSDMEGLEDPNLVDYIRTFGRKGK"
     gene            complement(38725..39864)
                     /gene="bshA"
                     /locus_tag="PK742_00415"
     CDS             complement(38725..39864)
                     /gene="bshA"
                     /locus_tag="PK742_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HQU75078.1"
                     /translation="MKIGIVCYPTYGGSGVVATELGKALADKGHQVHFITYKQPFRLD
                     SFHENIFYHEVNFSNYPLFDYPPYETALASKIVDIAEYEQLELLHVHYAIPHASAAYL
                     AKQILKEKGYHLPFITTLHGTDITLVGKDETFVPVVTFSINKSDGITTVSQSLKDTTY
                     EHFDIRKDIEVIPNFIDFSRFKKLDKDHFRKVIASNGEKIIMHTSNFRKVKRVDDVVR
                     VFCKIRQEMPAKLLLVGDGPERQNIEKMSRQVCHQDDVHFLGKQDAIEELLAISDLFI
                     LPSETESFGLAALEAMACQVPVISSNTGGLPEINIHGETGFLSPVGDVDDMAANAIRI
                     LENEETLAAFKERAFKQAQRFSLDRIMPLYEAYYIRVLEQASATA"
     gene            complement(39966..40871)
                     /locus_tag="PK742_00420"
     CDS             complement(39966..40871)
                     /locus_tag="PK742_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HQU75079.1"
                     /translation="MKDHILIAGGSGFVGQRLTSLLQESGHQVSWLSRKSGQEGAVNR
                     IAWDPSRKFLGENAFDGITRLVILSGEGIADKRWTDQRKQLIIDSRVQGISLLKEALL
                     SRSHTVRSIVSASAVGFYGTRGHELLDETSTPGAGFLSTSVQTWEEAAHGLEETGLPV
                     AIMRIGLVLGRGSGVWQEMDNYLRFGIRPIFAAGKQWWPWIHLEDVAGIFQHSVKQEL
                     SGIFNAVAPGIQDQKTILGAMSAARGGFSIPAPAPPFVLRLLVGEMSTAVLASQRVSP
                     QKILDTGYRFRFPDLTAAMQDLYRK"
     gene            40967..42817
                     /locus_tag="PK742_00425"
     CDS             40967..42817
                     /locus_tag="PK742_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQU75080.1"
                     /translation="MLRPTLFLSVVITLPFLFSCKGNKTASEHADIIGIDSSRYIRIP
                     DADSLLPGWRTDNSVVNHWIGDPDNLHPTNGRTASRSWILSLTHNYLMRLDPVSMEVV
                     PDLATAQPEISANKLEFTYSIRPDARWDDGKPVTAADVAFTLKVNKCPLTDNPNARSE
                     TENLMDIRLYPDDPQKLTLIMHHEYLLNIAFLATFAIMERDFHDRDQVMDGVSFAQLE
                     DPAWLENPPADVVAFMQEFNDGKYGNDPSLYNGSGPYKVVTWNRHADLILDKKPNHWT
                     DQIKDPKVFQQAKNDRIVFLEIKDDNAQMLELKSQTIDATVWLSTQSMLALEADENFK
                     RNYNYRYTDNYSFNYLGVNTQPDGIRHPKIFDSKEVRQAMTYLVPIEDIVHVIYNDRA
                     TVWPAFLSPYKNGFNDTLKPYYHDLNKAVSLLENAGWTDTDGDGIRDKMVDGKKTDMR
                     FELLYSHAGAWIDQMAGMIREAFLAGGIDVKVMVMDLPALSQKVSSHDFDAYLGAWGT
                     NSLPDDYTALWHTSSYAGGGPNFCGFGNTYTDALIDSMNLVVDESVRAPMIRELQAIV
                     HDEAPYIMYLSASRKNVIHKRLGNQIMTFERPGNMVNYLQVIGHGMQELQ"
     gene            42830..>43114
                     /locus_tag="PK742_00430"
     CDS             42830..>43114
                     /locus_tag="PK742_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75081.1"
                     /translation="MSKVLLRRLLFFLPTLVVISLLSFLISISAPGDPVDRLLPTAGT
                     SGETGLVSKRIMEERQAMRHRLGLDLPAFYLSVSSLSMAAEMPDFQNPEEE"
BASE COUNT        11002 a        10515 c        10517 g        11080 t
ORIGIN      
        1 cccgctggtc atcgtcgccg cgatatctta tgtgacggtc aagtatttcg aacctcatcc
       61 gatgccggtc agacaattga tggataaagg attgtttgcc ggatatgaca gcgacagccg
      121 tgccatcagc aaactggaca tcaccaaact gatcgagcag gattttcaga cagtacagcc
      181 gaatatgaac ctgggacagc tggtagaagt agtttcccac tgttcccgca atctctttcc
      241 tgtggtagat gaagagggcg ccttcagggg tatcattacc ctggatgaca tcaggacact
      301 gatgttcaaa ccggagcggt atgccgatac ttacgtcagt cagctcatgc atgcgccgcc
      361 cacagtgctg caaaagcagg agggaatgga tcctgccatg aagaagttcg atgaaaccgg
      421 tgcctggaat ctgcctgtgc tggatggaga taagtatctg gggatcatca gcaaatcacg
      481 gatttttggt gtttaccgga agcttctcag tgaagaatcc acacgtctgg aactctgatc
      541 agtgacctgc ctctcctgaa catttcaagc ttcatgatca ttttatgaca gataaaaagc
      601 tggctactta aagaaatgct taattttatt ggttgattca acgtaaggcg tatgaaggga
      661 aaaactactc tcctcctttt tggactttta ctggtcactt cgcttcaggc acaggtggtc
      721 atcaatgagt tctcagcagc taactacagt gatgtagctg ataactatgg tgattttgaa
      781 gactggatcg aactctataa tgccggtgca actgccgttg atctgggcgg ttacttcctg
      841 agcgataatg cttccaaccc tgacaagtgg gagattcctg caggaaccac gatcaacgct
      901 ggcggttttc tgcgattctg gtgttcgggg cgtgatgagg tatcaggatc caattaccac
      961 accagtttca agatcaccca gaccgttgtg gatgaagatg tggtatttac tgcccctgat
     1021 ggtgtgacca tcctggatgt gcaatatatt gaagagccca accagaagaa ccacagctgg
     1081 ggaagatatc ccaacggttc tgacaactgg tatatctaca cggatccttc acccaatgca
     1141 tccaatggtg ccactcacta tggcgcatat gccatcaaac ctgacatgga gccgaactcc
     1201 ggaaactata ccggatcggt tacggtgtct atcacttctc cgcaacctga cgtgaccatt
     1261 cgctacacca ccaatggttc ctggcctact gcagcatctc ctgtctatac cggacctatc
     1321 acgctggatg agaccacagt tatgcgtgtg gttggttttc atgccgatgg tgatacttta
     1381 caaagctggt gttctaccaa tacttacttt attgatgaaa gcaccaacgt gcctgtcatt
     1441 tccattagcg gtgaggaagt agatgacctg ctgaacggca atggatggct ccatccgatc
     1501 ggatcatttg agctttttga tgaaaacttc gtcttgcagg atgaagccat tggcgaattc
     1561 aatgaacacg gtaatgactc ctgggcttat gatcagcgtg gatttgactg gatagttcgt
     1621 gaccagttcg gatatgacaa cgatattgat ctggactttt tcagtgatgt aacagaccgc
     1681 acaggttacc aacgtcttat agtaaaagcc gcggccaacg ataattatcc ggaatcttca
     1741 ggaggggcac atgtgcgtga tgcgtatgtg catcatctga gtcaactggg cggtctcgat
     1801 ctggatgaac gttccacctt cttctgcctc gtttttctga acggaaatta ctggggggta
     1861 tatgactgtc gcgaaaaagt ggacgacaac gactacacag attactacta caagcaggat
     1921 gaatatgaaa tggacttcat caaatgctgg ggcggtacct gggcagagtt tggcaccatg
     1981 gctgactggg atccgttcgt caattttgtg accaccaatg acatgacaga cccggccaat
     2041 tatgcctatg ttactgacaa cctgaatgtg ttaagcctga tcgattacat ccaggtcaat
     2101 accgtatctg tttgtaaaga ctggctgaac tggaacaccg gatggtggag gggatatgac
     2161 cctgacggag gtgcacttac ctggcgttat attctttggg ataacgatgc cactttcggc
     2221 cattatataa attacacagg tattccggat gattcgcctg atgcagatcc atgtgacccc
     2281 ttcggcctgt atgtggaccc caatggtcat gtggacatgg tgaatgctct gctggagaat
     2341 gaagatttct tttccctgta tgtgaatcgc ttcgcagata tgaatgcaac cacatattcc
     2401 tgcgacatga tgctcagtgt actggacagc atgatgaatg tgatcgaacc cgaaatggag
     2461 agacatacag acaagtgggg aggcacttac agcggctgga tgtccaacta tgaagacctg
     2521 tatgaattca ttgaagagcg ttgtgcttat ctcgaagtgg aaggcatgga agactgtttt
     2581 gacacacctg catatccaat tgaagtacag atagacccgc ccggttcaga caatgaagta
     2641 aaagtgaata ccatcattcc tgctgacttt ccgttctctg ccgtctattt tggtggtgtc
     2701 accttcaatc tgacagcgct tcctgcttca ggttacattt ttgatcactg ggagtttctg
     2761 cataacacgc cttccagtac cacagatgaa gccataaccg taggcctgga aagttcagat
     2821 accattgttg cctattttac tgcagatgta ctgcctactt ataactttac cctggatatc
     2881 ttcccacctg atgccggtac ggctacagtt attggagtaa cgcccggtac ctatccatat
     2941 tccaacagtt atctgagcgg taccatagta gatatgcagg ccagtgccaa tgccggatac
     3001 gtctttgatt actgggagct ggataatcac ctggtgaatc ccgatccgtt tgcagacgat
     3061 gttttcttca ttcagagcac agtagaagat gtactggtac atttccgcac caccacagcc
     3121 ataaatgatc ttgtggatgg aattgcggct tttgcagttg ttccttctct gacacagggc
     3181 aatatccagg tgcagtttga aatgacacaa gcaggagaag taagcatcga cctgttcacc
     3241 atgactggtt cctttgcagg caacctgagc aacggctacc agccggctgg tgagcacagt
     3301 ctctcctaca acctgcagga tatgaacctg gctccgggaa tgtattttgt aaggatacag
     3361 tcaaatggaa tcagctacaa ccagcagata gtttacagca gataatagtt tagtttaaga
     3421 cataaaaaag gctgcgcaaa tgcgcagcct tttttatgta ctatgagctc tcttttatcg
     3481 gaagaatatc ttttcgctgt acactacagt tccattgtct gtcagttgca acagatacag
     3541 accagatgcc cattgcttcc ggtctatgac cgcaagcctg tcgccggcat acacttgttg
     3601 tgtcagtacc acctgaccac tgatatcagt caactgcagc atcaggtcac ggccaaacac
     3661 ctcttcactt ctgaccagca cctgctcatt ggccggattg gggaaagatt gtatgcctat
     3721 taatggttcc tgttccggta cggaggaagg caggttcaat ccggcagtat cttccagata
     3781 gaaatagatg agaaccatca tttcatcatc gctggtaagt ccaaaccaga caggagacgg
     3841 gccatcattg atataacttg cctcatgaat gacaccctca ctgactttta tgggcaggaa
     3901 agaatcccag tatctgatgg gcggatgctg ataatcatag atctcgtcaa tgcatcccgg
     3961 aataccgtct gtgctctcgc aggaagcatc aaagatctgc tcgcctttgg tgccgtcagc
     4021 tttgcgcttg tacacattgt aatctgttcc gtatttatgt gtatgactgg aaatacccca
     4081 aagaaagatc tcgtcatcac ctgaatcgta taaagattct tcaaaggtgt actccaaccc
     4141 gttattgggt atgtaaatgt ctgtattggc gggaagctct gtatgcattt cctgaacagc
     4201 agtacctgca ggctgcgtat agacattcat atacacctca caggcaagcg gcatagtgga
     4261 tgaatagttg atgtaatgag aattcagatc cagccaggta tctgattccc agggaaaagc
     4321 cgtattgggt ggcagatgca ggtcataact gaactgattg gcagtaacga gagaaacgcc
     4381 cagaaattcc ggatcgttgt cacgcagccc ataaggattc atcaaatacg ttggggcgtc
     4441 atatttatag atgataaaat gatgtgaata atcgcccatg atgacatcaa tacgatttac
     4501 ttccaggtct tccggtatct gagtagcgaa cttgctccag tattcatgtt ctgtctcagg
     4561 ccagagaaac caggggccat agtgtatctg gaagccttct tccggagccg gtggtgccgg
     4621 agggtccgga acgctttgta ttccgccctc atcataaaac gtagcaatga tggtagtatc
     4681 taccatattg cctgtttctt ttgctccaaa tatgatccat tgcctgatca gttcaatttg
     4741 tttgttatcc agtactggtt caccttcctg agggcaggcc gcaccttcgc cggctgtcag
     4801 gttcacatcc agagccagtc cgttgttgat cttgctgaac aaaaaacttt tatagggatc
     4861 gcccggagag atcactttca ttcccttccc cgcagcttca gcattgaccg gtatggcatc
     4921 atagatagca tcatacactt cggctgcaga gccattcagg gtaagaccgc tttcgtgtgc
     4981 ttctatatgg caggaaccac atttggcaga aatgatatca tacacctgca gatatgtgtc
     5041 ctgtgcatgc aatccaacta tagacatgca caaaggcaaa gccagcaata cttttttcat
     5101 gatcttcaaa tataaggcaa aaatggcctg acacgagcaa gtctggccaa tttgaatcaa
     5161 tcatgacaag gattaagaga tcttatcatc cctgaaccag tctgcataac gagtgtagtt
     5221 attggcgatt cgttctatat tatgcctgaa tacttcaggg gtcagttcct tgactttttt
     5281 agcaggaact cctgcataca caaagcccga ttccaatata gtatgttcca gcaccactgc
     5341 acctgcggca atcagtactt cactgtgaac cacggcgtgg tccatgacta tagctcccat
     5401 acctaccagc acacgatcgc tgatggtgca tccgtgtaca atggcattat gcccgatact
     5461 gacttcgttt ccaatagtgg ttggtgcggt ctggaaggta caatggatga ccgcattatc
     5521 ctggatattc accttgttac ctatccggat gctgttcaca tcgccgcgaa ctactgcatt
     5581 gaaccataca ctgcaatcat cgcctgtaac cacatcaccc acaatggtgg cattcggcgc
     5641 cagaaaacaa cgttcaccga aaaccgggtt ctttccttca accggcagca gcagagccat
     5701 gagttttttg tttagccata aaggcgaaga tagccgtttt ggctgcttca taacctgtct
     5761 gataaatatc atctgcccgg ctcaggtcaa aaattccaaa acgacttacc aggggttcaa
     5821 tggcaatatc acagcgcttc agtctgttct gtacacgttc ctgcagactg atatcaagac
     5881 agcgtacacc cacatcacga atgccgttaa cctgatcaat gtattcaacc gtattcacat
     5941 tcacaccgat gagaatacgg catttatcac gcaaaggctc aaccggcagg ttgttgatga
     6001 ccccaccatc tacatacaga tgtccgttga tctgaaccgg tttgaacacc atagggatgg
     6061 atgcagaagc gatcacatga tctaccagct tatcaccctg atgtatgatc tcgcagccac
     6121 ctttgttgag attggttacg gccacataga aaggttttcg caacttactg aaatcatcac
     6181 ccggaagatg tttgtacagg acatcacgga agtatgataa ttccatcatc cctttattgg
     6241 gaagccccat tttcacaatg ctgtacaaag acttgtttct gataagggaa aaaatggcat
     6301 ctgcagacag cccggctgca tgtagcacac ccacaatagc gccagcgctg cttcccgaga
     6361 tcacctcggg ttcaacagct gcttcttcca gtgctttaag tacaccgatg tgagcaattc
     6421 ccctggtgcc tccgccagac aaaaccaatc cagtctgata aaccatttat agatggaacg
     6481 acagcctgtg caaaaagata cgatgctgct tacttttttt tgatgactga ggtcttttcg
     6541 ccaaatcgca gcgcaaaatc acggatctct gcagccagtt tctcgtcgtt attggctttc
     6601 agataggtat ctgccatggt aaccagggtc tgaaagaaaa atacattcat ttcttccagc
     6661 tgcatatcct tggtccacag atctattcga agggtctctt tataagccgg atcccaaagg
     6721 ctgatcagaa aagctttgct tacctgtggc tcagcagccg tgctgtcgct ggcctgccag
     6781 gtgatctgct cgggcagttg gtcatcatcc agttgtattc tgatcctgat ctctgactct
     6841 ttactcatat gatcttagtc ctgtttggca cgaaattagc gaaagtacag gcaggtgacg
     6901 ccccacaggt tgcacctcac ctcaaagcca tgtattttta cctaaaatca aacaccatga
     6961 aagtgtacca ccgcctgatg atcttttccg ttttcatact gacaggattg gttgcaaagt
     7021 cgcaggtggt ctatgtgacc gaatggaaaa gcgatgcaga caagatcgtt tacgtatctg
     7081 aatataccag tgaggccgat gtagtagtat tccgcaccga gtggaaaagc gatgccaaac
     7141 ccatgagtgg tatctggtat ttcacagaat ggaagagcga tgctgatttg cgcatttact
     7201 ttacggaatg gcgcagtgaa gcagaccttg tcatagccta tacagactgg aaaagcgacg
     7261 ctgtctggcg aaagaaatag gcatgttgca tctggtggct gacctgggga ataccacagg
     7321 caaatttgcc cttttaaagg atggtgaatt gcttagtatg catcggaccg ccgatcgtgg
     7381 aacggatgct ctccgtactt ttctggctga ttctgttcct gatgcctgga taatcagcag
     7441 tgtgcggact gacagttctg aaataactgc ctatttacag gatcttgcgc ccggattcgt
     7501 tctggatcat cagatgcctc ttcccatcaa cattacttat gaaacacctg aaaccctggg
     7561 gcatgaccgt atagctgcga tggttggagc ctggaaaatg tttcccggca aaccggttct
     7621 ggtgatcgat gccggcacct gcatcaaata tgacattctc ctgcctgatg gaagttttcc
     7681 cggtggcacc attgcaccgg gtttacgcat gaggctggag gccatgcata cttttaccgg
     7741 caggctgccg tttgtggaga tacctgaaca aacctggcct gaagggccgc cagttacagg
     7801 caccagcaca gccggcagcc ttctttccgg tgctctggca gcaggtttac tggaagccga
     7861 aggaatgata gcctactacc aacagaatca gccatacctt aaagtattgt taacaggagg
     7921 agatgctcca ttctttgcat cccgcctgaa aagtcagata tttgcagtcc cggaactgat
     7981 cctcagcgga ttggatgaaa tactccggtt tcaattcaac gcaatttgat gcatttttca
     8041 cgcagtttgt tcctggcctt tgctttggcc tgtactgccc ccgcatttgc ccagttaaac
     8101 tcaccctatt ccagaatagg cctgggaaat atctacactc ctaccatggg tggaagcatg
     8161 gggctggcag gggccaccgg tgctaatctg ttgaccacag agatcagtgc ccaaaacccg
     8221 gcaacctact gctatttgtt caaaagtgtg ctggatgtgg gcattagcgg ccgatttctg
     8281 acactggacg acggaacaga aacctttacc agcggcgaag gcaacctgtc acacctggca
     8341 ttcggctttt cacccgataa caatttttcc aaacacgact ggggtttcag caccggcata
     8401 cttccggttt ccacctcaca atacaatata gaacaaacca ccgcttcagc agataccttg
     8461 ctgggcgacc tgatgtcgca gtacacaggt acaggaggct tgttcaaata ttttgtcggt
     8521 ttgtcatacg gattagagtt taacaaagac acccttgtaa cactgaacaa gaaaggtgag
     8581 gcagacagca gccgctttta ccggaatgag ttcagtatag gtatgaacta cgaatacctt
     8641 ttcggttcca tttacaatac caccattgcc tccttcccgg atcaggtaaa ctctgttgac
     8701 acgaaatatc agcgccagac ctccatgcag ggtggtggca tcagagcggg aataacctat
     8761 cagcgcaggg ttggccgggt gaacaacctc aatgcaggag caagttttgc tccggggctt
     8821 gaccttcgcg gaacccaatc agtaagctgg ttcaatatca atcagtttgg cactatacaa
     8881 cagataacag ataccttgta ttatgcaccc gatgtggcgg gcactattac tacggcaccg
     8941 gaactgaata tcgggctggc attcaacaac tacaggcaac gaagcatcga ttcggtcaaa
     9001 tttcatgtta cggcagaatt cagctatcag gactggagca agtatgccgg tttcctgtat
     9061 tctgacagcc tggcttccat gtaccggatc aaagttggcg cagaggttat tccgccccgc
     9121 cgtgatgaag tgggaaaaag ccttattccc atcatctacc gggcaggatt ctattatgga
     9181 acaagctatc tggtcattga cggacagcag ctaacagaat ttggaatgac ttttggaaca
     9241 ggtattccga tgagaggaag cagggtcaat ctggcagttg gattcagtca gcggggagct
     9301 gccggtctca tacgtgaaaa ctatgtgaac ctgcaagcag gattcaacat ttcagactat
     9361 aactggttta tcaaacgaaa acaaaactaa attcaagaca atgagatctt tggtaatgaa
     9421 atcatggttg atgctattcc tcggtgctgc cgcattgcag gcagcagcgg gcgaagatga
     9481 cctcagctgg tcattacagg acacctgccg tgcggaagct gaaaatcagg aaaaatttct
     9541 ggaagaatac tccacttacc gtgaattctt caaacaggag aactataaag acgctctgat
     9601 acattggcgc tatgtgatca aggaagctcc cggctttcgc aagcagccct tcatagatgg
     9661 tatcagcatg tatgaaacat ttgtggaagc tgagcaggac agcgcccgcc gtgagctgct
     9721 cattgatact gtattcatgc tgtatgacaa gcgtattcag tgtcatgatg aagcaggatt
     9781 cgtgctcgga agaaagggtt acagcatgta caaactccgg tcaaaagatt acggaacgat
     9841 ctatgagacc ctgaaggaat ctttcagcct gcagggtaat aaggctgagt actttctcat
     9901 tcccacctat ttcaattata ccatcataca gtggaaactg aaaaaactgc agaatgatga
     9961 cgtgctcaat acctatgatg aactggtagg gatcattgag tataaccttg ccaacaatgc
    10021 agacgaggca gagtattatg caaagatgcg tgatgccatt gaccagatgc tgcctgccaa
    10081 cctgctgtct tgtgaagccc tgattgaaag ggtcaacagc cgctgggagc aggacaagga
    10141 cgatgtcaat aaaataacac gatactatta cagcatgcgt attgcccgcg acagcagtgg
    10201 caacagctgc ctggaaacca aagaatatga aatggtggtt cgccggctgt atgaactggc
    10261 ccccagttct gaattggcag aacaggtagc agtgtacctc tttaaggagg gcaaagagaa
    10321 tgaagccatt gaaaagttca atgaggccat tgagatggaa acagatgccg ataagaaagg
    10381 cagcctctgt ctgaacattg cccgcatttt acgcagccag aataagttca gccagtccag
    10441 aacctatgcc tataaagcac tggactacaa accaagctgg ggagaaccct atctgctcat
    10501 tggcgatctg tatgcctcca gtggtccgct ttgtggcccc agaactggtt ttgacagcca
    10561 ggtggtagtt tgggtggccc tggataaata ctataaagcc aagtcagtgg atccttctgt
    10621 agcagatgat gccaatgaca aaatagcccg gtattcacag tacatgccaa ccaaaacaga
    10681 cctttttgaa cgtggtatcg atgaaggcag ttcctatact gtaggctgct ggatcaatga
    10741 atcaaccaca gttcgcggca gaaagagcaa ttaataaaca agcatacctg tttgaacacc
    10801 cggcttatgc cgggtgttct tgtttaactt agcatcatgt tcggccggtt gtatttcatc
    10861 tgctgtgtcc tgcttctggc aggctgcgtc aatgatatgt ctgaagtgga acaaacggcc
    10921 gctctggctg accctggtat tgaaaaaggg aaagatgtgg tgctgtacta ttcagaagat
    10981 gggatggtaa aaatgtgtat ccaggccact accctttggc gctatcttac tgatgatcct
    11041 tatgtggagt ttaaagatgg catgaaggta gaattcttca atgatgaaga aaaggtcatt
    11101 tccaccctca cctccggcta tggaataagg tatgaaaaac agctcaagac cattgttcgt
    11161 gatcaggtag tggtgatcaa tgagaaccag gagaaactgg aaacagaaga attggtttgg
    11221 gatgaaaaaa aacatattat attcacagat aaatatgtgt ctatcacgac ggccgatgaa
    11281 gtgctatatg gcaccggtct ggaggctgat gagaccatga ccgaatacac tatcctgcac
    11341 ccggaaggga tcattaaaat tgacatggac gatgctcaga ctgatgaaga tctttgaagt
    11401 tctctggctt gcagccagcc tgattgccct gaccatggcc atcatcaggg ctacatcagg
    11461 ccaggcatat ggcaactaca tctatatcac cctctttacg gcagcggtag ccttattcat
    11521 gtacagattc aagaaacgca accgtctgta tctggaagag cgtgaaaaag aacatatcag
    11581 gcagcaggag gaattgcaaa agaaacatga ataatctgac ccagcgcatt ctgacaggtg
    11641 tggttggcgc agctgttatc atctttcttc tatgctggca tgcatggagc ctgaccattc
    11701 tgtttctgtt gctggccatg ttcacgcagt ttgaatttct gcgtgttgta aacaaactca
    11761 aagaaaaggc agatagtcct gcccatattt atggcaacct gtttgccggt ttgctgaatt
    11821 tcttactcat taccggtagc atggcaggct ggcttcccgg aatggcctgg accggaaccc
    11881 taccagtact ggccgccatc tttctgctgg agctatatca ggatcgccct aatccctttc
    11941 agcatattgg ctttcggcta accgggcaga tctatatcat attgcctttt gcctggatgg
    12001 cagttttagg tactgcgggt aacagctttc aatacctgta cccaatgggt gtgttgttca
    12061 tcatctggac caatgatgtg tttgcctatt ttaccggaag attactggga aggaacaggc
    12121 tttttccacg aatttcgcct gccaagacct gggaaggttt cattggcggg gtgatcatgg
    12181 ccctggtttg tgcatccatt tttgaccatt ttgatcaaaa ccagccgctg tttgtctggc
    12241 tcgtactggg attcctgctg gcagtggtag gtaccggcgg agaccttgtt gaaagtctgc
    12301 tcaaacgcaa cctccagatg aaagattccg gcagcatttt gcccggccat ggaggcttcc
    12361 tcgaccggtt tgatgccctg cttctgggtg caccggtagc atttgcctgg ctcgcaatct
    12421 ggatcttctg aaccttcagc aggcctgaat tgtattattt ttgcaactac aactatgttc
    12481 aggatcatac acaaagaagg cagaaccatt ctggccatat tcggcctgct gctggtagcg
    12541 ctcaacatcg tgctgcaacg catgtttccg gaagacctct ccctgcatat catagctctt
    12601 gttgtttcat tgggagcgct gttctttgtt ttatatttct tccgtaaccc gaaacgcctg
    12661 ttgttcattg aagaaggagc aatcattgct ccggccgacg gtaagatcgt agtggtggaa
    12721 gaaactgatg aaccggagta cttcggcgac cgcaggatac aggtatccat ctttatgagt
    12781 gtcaagaacg tacacgtgaa ccgaaccccc atcggaggtc aggtgaaata cttccgctat
    12841 catccaggaa aatacctcgt tgcctggcat ccaaaatcat ccaccgaaaa tgaacgcacc
    12901 acactggtca tagaagacaa cgatgatcag gtggagatac tcatgaggca gatagccgga
    12961 accgttgccc gcaggatcaa attttatgtg gaggagggtg aagatgttag tcagggtgca
    13021 gagttcgggt ttatcaaatt tggcagccgg gtggatctgt tccttcctct tcatgctata
    13081 gtggaggtca ctccgggaca aaaggtcaag ggcgggcaaa ctgtcattgc acgcattccc
    13141 ggaaagcatt aaattgcagt ctaataatat ggttatgaaa aggttctttc ttgtgatcgc
    13201 aatactgggt gccggtgtta ttgctgccca ggctcaatcc aatacttcag ttgctcctgc
    13261 cagtgatgct actccggtag tgaacgctgc agatcatatg ggtcagggca agtcctgctg
    13321 cagtgctgcc ggtcaggaaa agcaatcctg cactgcagaa aaagctgcat ctgctgctaa
    13381 agaccagaag aaaggcggtt cctgctgttc tgcaggtggt aatgcagagg ctcaatcagg
    13441 caagtcatgc gagggtgtaa gctttggcat gggtgctgcc aaaccgtcta aagacgaata
    13501 gtcttagagc atagctccta ttgccttaag ctctgttact gcacagttaa acctgtgggt
    13561 gttttgttct ttcttatagt taaaccagat gaaagacata cctgcattca tggaaccgtg
    13621 cccgtctgca tcaggagaat ctcctatata gagactttct gaagcttctg ctccagcctg
    13681 acgcattgca tgttcgaata tcttttttcc gggtttgagc tgacctgcac tttctgctgt
    13741 ggtgatggat ctgaaatact ttctgagccc ggtgtttacc agtttgaaat cctgcacttc
    13801 ttcaaaacca ttggtgatga tgtgcagcgg atattttgca gataactctt cgagcagctc
    13861 gaccgctccg ggtaccaggt ctttcaccct tcttgtgttc tcaatatagt aatctgccag
    13921 acgcatagcc ataaggcggt tatctattcc atgatcatat aaagtatgtt gaaaacgttc
    13981 cagccgcacc tcttctctgg ttttgttgcc cagtttgtat tctgaccaga gatggctgtt
    14041 acgcattcgg tactgatcat gaaaactatc gaaggcatgt atgcccatct cctgcagatc
    14101 aaaagcctga tacatctcat aaagaatgcg atgcgaatta gctgagaaat cccacagggt
    14161 attatccagg tcaaagaaaa tatgtcgtat atgtgaaaaa ttatgcatgt tcaaatgata
    14221 gagggtgatg acatccatcc tgctgtgtag cctgaaagca gacacatgaa tctctgatgg
    14281 tttcttccag cgaacgaaat tccatgccca gtctgctctt cactttcgat gaatcgtacc
    14341 gggtctgtct gttggccata cgggctgttg ttctggtgat ggccttgttt cccagaccaa
    14401 acatcctgag tatagcatta agtcgccagg ccaggccact tagtttcggg cctgcttccc
    14461 acctgggtgg aggcacctgc agatacttcg ccatggtttc aaagagatgc tgataggtca
    14521 ggttgtctgc actgagcaga aaacgttcgc cgaaatgttt ttcttccatc agctgatggg
    14581 ccatttgcac cacatcacgc acatctacaa atccggtaat accttgtgta tagaatggat
    14641 agcgtttata gaccgagcgg aataatcgtc cgctgctttg ctcccagtcg catgcaccta
    14701 aaatgatgga ggggttgacg atgaccgcct gtaatccttc tgccacacca cgccaaactt
    14761 cctgctcagc cctgaatttg ctcacagcat aatcagaaag tgctttccgg ctttcccatt
    14821 tgttctgttc ggtgatgacc tcgccacggg tgtatttttt accaatagcc gccactgaac
    14881 tgaggtgcag catggggatg tttctgtcca gacaggcgtt caccactgcc tctgtacctg
    14941 ctgcattgat ctggtgcaac cggttcttgt cttccttttt ataagatacc aggccggcac
    15001 aatggtacac cgcatccacc ccttctgtgg catcagacag ggcaaatatg tcgcggatat
    15061 ctccgcagaa tttttccagg ccctctactt cttccagcag ataccaggag ctttctgacc
    15121 ggtgcaggat acgcacttca tgccccagtt gcctgaggta cctgaccata taacttccgg
    15181 taaatccggt ggctcctgtt accagaattc ggctcattgt tcagttggat aggatatcac
    15241 acccccgctt actgctgaac gggcaccggt tacggagggc aggaaatttg gcatttccca
    15301 ccaccgcagt gcgcccatca gcgccatggc aagcgcttcc ttgtagcgga tgatgtggtc
    15361 atccggtaaa gatacatgat gcctgctcag ggctctgatg cgttctgtca acaggtcatt
    15421 gtaagctccg cctcctgtaa gcagcaccct tccatttttc tgatgcaggt ccatggcctg
    15481 tgctatctgt aaggcaatat gttctgtggc cgtgcgcagc ctgtcaggca aggagtggtc
    15541 atctgcctta agtacccgca cccagttttg ctgaacatat gcattatcca gcgaacgggg
    15601 aggaggcaga cagaagaaat catcggcatt taactgatgc agaaggctct cagctacccg
    15661 gcctgatgct gccatgcttc cccgctcatc aaattcacgg ccggcttctt cggcaagtgc
    15721 attaagcaac tgattggcgc cgcaaatatc aaatccgata cgacggccat cgagttccat
    15781 tgatacattc gcaatgccgc caatgttaag acataattga tactgcggaa agaggagctc
    15841 atcacagatt ggaacaagcg gagcaccctg gcctccggca gcaatatcag ccatgcggaa
    15901 atcccagatg accggaagtc ccgtaagatc tgccagtatt tgaccgttac cgatctgcaa
    15961 agtgtggcca gcctccggat gatggctcag cgtatgcccg tgacttgcaa tgagatcagg
    16021 gttatccagc tggtgtcgag acaaaaatgc ctgaatctct gcgcccatga aggctgcgag
    16081 ttgagcagat acctctaagg tatcatttcg gtctgtgctt ccgctgtaag ttttcagcag
    16141 ttgcctgagt ggggcaggaa agggaatggt ttcagctgcg tctatggaat aaagccagtc
    16201 agttctggtg atggtgcaga ccgctatatc caacccgtca agagaggagc cagacattga
    16261 tcccaatacc ctcaccttat tattcgtcat gcagggtttc tatatatgct gcaacctgat
    16321 cgatctcttc tgcagtaaga atatccttgt ggggtgccat tccgttccgg ccgaaggtga
    16381 tcaccccgat tttttcatct ggtgtaaggg tggactttct gaggttggcc gcaccgctca
    16441 gacccagatc acctttgctt ccatgacaga tcttgcagtt cttctgatat actgcctgac
    16501 ccggcagttc attggtggca gcctgagcag cctgttgatt gccgcttggt ctatttccgt
    16561 tgctgttgtt gcaggcggat atcagaaaag cagtaacaaa aaataaaacc ggtagccttg
    16621 ccatgagttg gaaacagtgc aaagctaccg gttgttttat taagaaatca tcgtaccaga
    16681 atgaacgaac cctgcagatt gatcagatct ccggtaatat ctttcgctct caccacatat
    16741 acataggcgc ccacatcctg tggttcatcg ttgtatttcc catcccaccc gaaagtagga
    16801 tcattggtag taaagagttg ctgcccccac cggttgtaga tggaaaactc ataaaaatct
    16861 gccacggtat gggtgtacac aaagaacagg tcattaacac cgtcatcgtt tggtgagaag
    16921 gcattaggca catctacaat ggtgatgtct tccacataaa tagtaaccgt atcactggca
    16981 ctgcaaccat ccgggctggt cacggtcagg atataatcat aggtctgatc aggcctgatt
    17041 tgcgggtttg caatgaacgg attgctgaca aatgctgccg gcgaccagct gaaggtacca
    17101 ttgccttctc ccatcagggt gtaatactcc agataggtaa cagtatcatc caatccggca
    17161 tccggataag gcagctcacc tatagtgatg gattgtgtaa tagtgtcctg gcagccattg
    17221 ttggtggcca caatgagtgt cacctcatag gtacccggtt cctcgtagct ataggaagga
    17281 tcctggtcac ccgaggtacc accatcacca aaatcccaga accagtctgt cagggtaaca
    17341 ccatcggggt gcgtggtctc atcttcgaac aaggcgtctt cgttcaggca cacatcgtca
    17401 gcagaaaaat ctgcagtcgg tcctgacagc aggggcacat ccagcaagac ggtgctgctg
    17461 catcctttat cagtggttac ggtaagtgct acttcataaa ttcctccgat ggtataatcg
    17521 tgcgatggat tttctgcggt actggaacca ccatcaccga aatcccattc ccaggaaatg
    17581 atctcacccg cttctgtgga ggtagatgca tcagaaaact gcacagcttc accagaacaa
    17641 ccggcattga attcaaaatc tgccgtaagg gtattaaaaa tatggatctc aacaaccgaa
    17701 gtatcagcgc atacgaaacc cggattacag atcagtgaaa cagtgtacac tccggtatct
    17761 ccatacacgt gcaccggctc ataatcttct gaggttgcac catcgccaaa gagccacaga
    17821 taactatctg ccccggtgct gaagttgctg aatgggatgg taaagtcttc gcaatccagg
    17881 tacacatcgg ttacgatctc accatctacg cccagggttg aaacaagtgc aggttcacag
    17941 agctggacat tgaactgaaa gtctcggata tgattaccca gcaagactcc gtctcgccat
    18001 tcttccacca ccacgccaag cacatagcgt ccttcatctg tagctgtacc ggtaagcaaa
    18061 ccggtattca catcgatctc aaaaccagga gcaccatcta ccatgtagag tacgtcatat
    18121 ccggctgacc atgttacggg gtcgtaggga ggaggaggag gcggaaatgg tgccggatca
    18181 aatggactgg ctcctgcata tggtgtaaag aatttatagg ccagtgaatc gccgtcagga
    18241 tctgttgccg agtgatcgaa actgatcggg tcgcccacac agatcacgct tggcggatag
    18301 ttggtaaaga ccggcgaact gttgcaggaa gcgtcttctc cgggcggtat ctgttgccac
    18361 atggtagagc cggtatcttc aggacccaca atatttacaa tggatgcatt gcggcaacag
    18421 cgttgataga ccaggtcgaa aacctgaaga tcatcaggca cagttatgga aaccgtgtag
    18481 atagcctgtt ccacacacaa accaggtggc acgatcagac agggattatc cacttcgatg
    18541 ggcacatcag aaagtaccgg cgagaacatc gaaacagtgt ttagaagggt accgtcttcg
    18601 tcataaatac ctaaataggc tgggtcgtca aatggagttc ccccacctat gggagaacag
    18661 tctctgtaca acacaagggt cacatcatag gtattaggtc ctgtgcaggt gtagtatagc
    18721 tctcctccaa taatatgcgt ggcataggtg gtgtgagaga caagaaggat gaccagagac
    18781 agcagtgtgt agcgtaaagc gttcatgctc aatagtgtct ttactttaaa gttacatatt
    18841 aattgagtaa ggtgatgtta cctgaataaa cagcctgctg accttcacat gctattgtaa
    18901 tatagtatat atacacacct gtctcctgtt tctttccgtc ggtggttcca tcccaggcag
    18961 tatcaatact gccagcttca aaaaccagct ctccccatct gttatagatc tggtagttgc
    19021 tcacttcatc atctccaaaa ctgctgatgc ggaatacatc attaagtccg tcaccatttg
    19081 gtgaaaaggc ggtaggaata gtaaatcgca tacaacgcag ccatacaact atactcacgg
    19141 tgtctgtagc tgtacaaccg ttctcagcgg taacagtgac cacatagctg tctgtttcag
    19201 atggcatgac caccggatcc tggctgaaag ggtcattcat atagttggga ggatcccagc
    19261 tgtaactcac tccaccggca gcatgcaaca tcactggttc aaaccggtac accgcagtat
    19321 cattccatgc cactgcgtct ggagctggca gtacttctat aatgatctga gctgtgtcac
    19381 tggtgcagtc atcactgatc acgaccgtat aggtggtaga aacatccgga tacacaaaag
    19441 gatcatgatc cagcagatca ctgatgttgt agttgggtga ccattgatag tatttaccgt
    19501 tccgggtaaa aagctgaatg gtatcgcctt cacaaatagt agaatcgcga aacacgtcgg
    19561 ccactacttt gggatagacc acaacctggt tggtgatact gtccatgcac cctttgttac
    19621 ttgtagctac gagggtgaca ggatatgcac ctgcggtatc aaagtaataa gcaccgaaag
    19681 aacccgtgct gcctgtacca tccgggtagg tccattccag tccggtaatg gtgtaggcag
    19741 aatctatgaa cacctgctct gtaagtgtac ctacagattg caggcaggca tcatcccaca
    19801 caaaatccac atcgggcgta ggccacacgg taactgtatg ctccacacct tttgtacacc
    19861 cgtatccgtt caatatctgc aaatgcactt cataggtacc tccctcctca tagacatgag
    19921 aaggtgtatt gccggtgcct tctgtgccat cgccaaaact ccagctccac tgttccagat
    19981 atccgtatgt actcactgat acatcactaa aattcagggg ctgtccggca cattgcggtt
    20041 cataggcaaa attggctacc agaccgggat agatctgcac tattgaatag gctgtatctg
    20101 cacagaccgt ataaggctta gccaccagca tcactgtgta ggtaccggta tcgggataga
    20161 cataactggg cgacataact actgaagtat cattgtcaac cgaaggatcg ccaaagtccc
    20221 agaaccaggt cgttgcaccg aatgaattgt tgtcaaaaaa gatggtataa tcgtcgcagt
    20281 tgttatactc atcaggaaaa tctgcatata aacccggggt acaatcggtt acgttaaact
    20341 gaaaatcacg gtagtgttca ccaatcagca ctccatcacg gtattctttg acacaaactc
    20401 ccacaacata ctgcccgata gacgcaggtg taccttccat ccacccggtc agtgaatcta
    20461 tgaccagcgg aggggaggca tccagcggat atccttcaga ataaccacct gtataagtaa
    20521 catatggaaa cggaggtggt gatggaattt caggagcagg aacatcaaaa ctggctccct
    20581 ggtagggtct gcaaaattcg tacaccagct gatcgccgtc aggatctgat gcagcgtggt
    20641 caaaattaaa cgggtaaccg gcacatatga ccacaggagg aaaggaattg aacctcggac
    20701 tgctattaca gatgcttccg ggatccggaa tatgggccgt ataggttgcg ccctgctgtt
    20761 caggaaatat cagattcaca atggtggagt tgcggcagca tctctgataa actatgtcat
    20821 accccagggg gttgtcaggc aaagtgatcg ttcttgtata gatagcttct tctacacaaa
    20881 catccggagg attgaccata catggattgg taagatccgg gtctatcaat gtggatccgg
    20941 gaaatggcag ttccagactt tgaaacagtg tgccatcgcc attataaaca gccatgtaca
    21001 gcggattgtc aaaaggggct ccatcgccac tgcaatcgcg gtacagtttc agggtgatga
    21061 gatactcatt ccctcccagg caatcataat acatctcgcc acccacaatg tggtaggctt
    21121 tggcaggcag tgccaggcca aagcagagta atatggataa ccaggatcgc accgggcatt
    21181 ctttgggtca ataaaagtaa aaattcagca gcggttcttg tgtaataaag aagtcatttg
    21241 atgtaaagga tcgctgcagc agagcagtat agcagtgagt ggtgagtggt gggccaggag
    21301 taacagggga accggataac aaaactgtca accgtattca ggacgctaca cgctacacac
    21361 tgataactac aaattgccaa ccgccagctc ctaatactac ctttgccaca tgcagatctt
    21421 catactggtg ctggccatca tcttcaccct catcgtggtg gtgggattat tcattccgag
    21481 aaaacaaacg ttcacagaaa cgaaagatat tgctgccgac aaacggcgca tcttcaacct
    21541 gatagacgat ctggacagct gggagacctg gagtgcctgg agcagcctga atgataccga
    21601 tctgcagaag gaatttcccg gaaaacacag cggctcgggt gcctcatttg agtggaaagg
    21661 aagcaaaatg ggaaagggca gcgtagagat cgttcgggca gaaccttatt cctccctcga
    21721 aattgccacc cagttccgca acaaaagatt ccgtgtcatc catcatatcc agctgcatgc
    21781 cgatcggttg cccgtacaac tgacctggac ggccacggtc aagacaggct ggagttcacc
    21841 gtcccgcatc ctgggcaggg cggtaatgaa caacatgcaa acggatattc acaagagtct
    21901 gatcctgctt agtcagttag ccggcactgc cgctgaagcg taaccgtatc tgctgaccga
    21961 tctgccggaa actctggaag ttgagcggga attccagcac cacatgcagg aagctcagga
    22021 agtacaacgc catcagcccg gtgcggtaat cggtgtaata cagcaccacg gcgatgatcc
    22081 acagggcgaa gatggccacc agctgtggtt tggatacttc gtgccatttg atgaccgatg
    22141 tcttgctgaa ccagttcagg taatgatagg tataggcgta ggcgatgaag cgggtgatca
    22201 gtatgcccgc actgctgtag tagatggcat cttcgccggc agcaggttcg tgcaggaagg
    22261 actgaaagat ctgggcgttc aatgagcgga aggatacgtc ataactgcgc ttggcatact
    22321 cactgatctg gtagccggca ccatcgggaa gaatgaaaaa gaagctaacc gcacagccga
    22381 tgaagaccag gatggagaga taaccactcg tactgtggct cttcaatgca cccaccagaa
    22441 tgaaggctcc ggtaaagagg aacacatgaa tgagtgtggg caggaagata gtaaagatga
    22501 cctgcgacag atcataattt ctgatcatat atcccacgat ggccagcaac acaaagctga
    22561 tgattcggta gttcagtttc ttgaccagga tcatgatgag gctggcactg aatgcaagga
    22621 acatgacagc gggattgagg tcgaagaaag tgtacaggct tttggaaatg agcggctcac
    22681 cgttgatgtc tttcggacca aaatgcagca tgataccggg cagggtcagg aagaaggtaa
    22741 gcagggtcag caggatccag tcgtacttgt tgggcgaaaa atattgtcgc ttatgcagcc
    22801 aggagatctc tgtcagataa tgcagcggtc ccagtacggc gtaagagaac aggaacagct
    22861 caaaggggat gacaaatgcc acagcagccg atatcagcat gagggcaatg ttgatgtagt
    22921 tcacacgggc gacggtcatg ggataaaatt agggaaaagt aaaaagggag catgcaaaaa
    22981 gaggaaagcc acaggctttc ctctttcttg ttcattttaa ttttcagtta tgttcatcag
    23041 gcttcaccgc cggcagattc tgtgcgcata ccgcccagtt ccttgtcaat gtagtagttc
    23101 gccttggggt cattgcccag cagtttgatc ttgtcaatga caccgcggaa cagggtctct
    23161 tcttcgtgtt gttccatcac gaaccactgc aaaaactggt ttgtaccgta atctttttcc
    23221 tgcagactga tatccaccag gttgttgatg ctggcggtca ctttttgctc atgtgccagg
    23281 atggcctcga ataattccag tacggaaccg aactgcaggg gtggctgctc cagggcagga
    23341 gcctgtgcat ggccaccgca gtcgttcaca tagtggaaca tcttcatcat gtgcatgcgt
    23401 tcttcatcag catgcgcata gagaaaactg gcaattcctg ccagcccttt gtggtcgcaa
    23461 aaggaggcag ctgataaata atagctggag gcatatcctt ccatcacgat ctgcgcattg
    23521 agcgcctctt ccattttttt agataacata tctggttcgg ttttacatac aagtaacagc
    23581 ctgaacagga atacgttcag acaagaaaaa cgctgggtgc aatttagact gattcaacac
    23641 tcagggagtg gtgagcgtct gcatggtgct gttctccacg ctgccgaatt ttggacaatc
    23701 gcatttgtca ccgcccaggt tgcaaccggc aacaagcagc atcgtggtca gtatgaccag
    23761 cataagaaac ggttttccaa tggtcttcat acccgcactt ttcatagttt tatggccgaa
    23821 gcgaatgtac catttttcct gaacacatgc cggctgaaac aagcaacaaa acagtcctgg
    23881 tagccccttt ggactgggga ctgggtcatg caacccgttg cataccggtc atccgtgagc
    23941 tgctttccat gggttgtcat gtggtcattg taacgagcgg gcgccccttg ttgttgttgc
    24001 aaaaggaatt tccggaggca gatttctatg acctgccctc ctacaatatc tactaccaga
    24061 agcggggcag ttttctggtc aaactgacca tacagctgcg caagatacta ggaggcatca
    24121 tgcgggaaaa acgggtcacc agccagctga tcgaacagta tgatccggtc atgatcattt
    24181 cagacaaccg ctatggtgtg cggagtaaac acgttcatac ggtattcatc agccaccaga
    24241 tgatgatcaa gataccgggg tctaaattca tcgaatggat cgtctggcaa tggctgaatt
    24301 ttcagcatcg ccgtttccat cgcatctgga taccggacgt ggcaggtgaa cccaacctgt
    24361 cgggcgacct gtctcatctc cacccggtac ttcccaacac caccttcatc ggcttgctga
    24421 acaggttctc cggcgaatac gacactaccc tgccacccaa tgatgtgctt tgcatcctgt
    24481 caggccctga accgcaacgc agcatctttg agaaaatgat cctggagcag gcaacagcca
    24541 tacgtcgcaa attcataatc gtacagggtc tttcagaaga gcgctccgat cagtgggtct
    24601 ctgaccatgt ccggctggtg gcacatgccg gagccagaaa actgcaccag ttggtggcaa
    24661 gttcacagat catcatctcc cgtggcggct atactaccct gatggatctg gcaccccttg
    24721 gcaagcattg cattttcgtg cccaccccgg gtcagaccga acaggaatac ctggtggaag
    24781 aactggcccg tcatgatctc tgtgtatatc aggaacaaaa acactttgac ctgttatcgg
    24841 ctctccaaca ggcagggtcc atcagaccct ttcagctgga agtggaggcc aacacttttg
    24901 tgccccagct tcatgcggaa tggcaggcag ccttacagcg aaaaatgatg tgacgctaca
    24961 gactcgccaa agaatatcgt ggcgtggcga ttgaaagatg cagggtcatc tgtggtgaag
    25021 aaggtgcgtt tgcccgtctt gctgcaacga ccagccatgt cgggatggcg ctccaggtag
    25081 tcatccagcc tttctgccac caccggaccc tgactcagca agtggatatg cgggggaagg
    25141 aaacggcgta tgaccggttc cagcagggga tagtgtgtac atcctaaaat gatagcatca
    25201 atgcgggggt cctgttccat cagctgctct accgaacgct gcacgaagta ctctgtgcct
    25261 gctccgtcca gttcgttgtt ctccaccagt ggcacccaca tgggacaggc ctgctggtgc
    25321 acttccacat ggggaaaaaa tcttccggct tcgatgacat aagacgcact gttcacggta
    25381 ccgttggtgg ccagtacacc tacctgtccg ctatgggtga gttgcccgat cagctctgta
    25441 gtaggacgga tgacacccag tactcttttt cctgatgcca tgcggggaag gtcctgctgc
    25501 tggatggtcc ggagtgcttt ggctgatgcg gtgttgcagg ccaggatgac cagctcacaa
    25561 cccatgtcga agaggtggaa gacgctttcg cgggtgaatt cgtacaccgt ctcgaaggag
    25621 cggttgccgt agggggtgcg ggcattatcg ccgaaataga cataatcata ctgcggcaac
    25681 ctgtccacga tggatcgcat gaccgtcagc ccgccgtagc cggaatcaaa aatgccgatg
    25741 ggtgttgcgc cgaggtccat aaggtaaagg tagtgagtag tgagtattgg gtagtgagaa
    25801 aagggttatt ggtattagca tatgtttcca attaccgaat aacaccttga aaagaacacg
    25861 tcattcctga agccgacgaa ggaggctatc aggaatctct tatctcattt accgttcact
    25921 cttataaact tttggtcaat agttctgtaa gagatccctg ctttcgcagg gatgacccgt
    25981 cctccggaaa aagtatcgtc aataaccagt aaacctcctc aaagcaatac tatggactca
    26041 ggactaatga ctgcagactg ctatcaggaa tctcttattt cggttaccgt tcactcctcc
    26101 aaagcgaatt gtcatccccc tgcctgccgg caggcagggg cttctttctg aagaatgagg
    26161 gaagaatgcc aggatctgga ttcacactat gagaggtata atttctttca agtcagaccc
    26221 cggcattgtt cgttcctcac aagctggtgt gacaaatact ttaattaaaa gagggataat
    26281 aatgcagcaa aatcaaacag gtgcgggtat aaacccgcac ctgtcatgca atgtatctgt
    26341 aatgcaatct gaaacttgtt tcgcttccgg accaaagacc acaaacaaaa aaccaacaaa
    26401 cactcctact tcccacccgg tgtcacaccg agaatacgga gcacatcatc gcttctgtca
    26461 ttcttctcat cagcaaagag tacagtggtg ctgttggcct tgtcaaagat gaaatcatag
    26521 ccgcgttcct tggccagttt ttcaatggca gcatatactt cgtcctggat cggcttgacc
    26581 agttcttccc ttttctgaaa gagctctcct tcgtaaccaa atttttcttt ctggtaatct
    26641 ttcatggcct tttctttggc ctcgatctcc tggatacgct gcttcttgac ctcttcagtc
    26701 aggaggtact gttcattctg aaacttgcgg tacaggtctt caatttcctt ctgcttcttg
    26761 gtgatctctg cactccattg tttagagatc tcatccacct gtttctgggc ttcttcaaaa
    26821 gcaggaatgt taccaagaat ataatctgtg ttcacatagg cgaagcgctg cgcaaaactg
    26881 ctgccggctg tggccagcag aaccaggagt acaagtaatg actttttcat acgatctctt
    26941 tttattcagg ttcaaaccct aataccacgg tgaacttgcc gttgttgaca atgtagttga
    27001 atacacctgt tgacggcacc agctctcccg gtagtttctc gtcaaatctt atgccataat
    27061 ccagaccaag caatccaaac ataggcagcc atgcccgcaa acccacgccc acactgcgtt
    27121 tcacatcaaa cggattgaac tcgttaaaac ttgaccagag gtttcctgct tctgcaaatg
    27181 catgtgcaga gatggttgca ctcggattca gactgatcgg gtagcgaagt tccagggtgt
    27241 acttggtaaa gatgggttcg gtgcgggcgg tgctgaatcc ggaagccaca ttgaagatct
    27301 catacccacg caaagcaatg atgtctgttc cgtagaagtt gaaattggta atgccatcgc
    27361 cacccagctg gaagcgttcg aaaggagata tcccgatatc cttgttgtag tatcccatca
    27421 gaccgaattt ggctgctgaa cggaatacca gcttacctac cacaggtgtg taccaatcgg
    27481 cagtgaaacg ccacttatgg tactcaataa atttatactt ctctgtgttc tccagtccgt
    27541 cataattctt ctcattgagc aagctgtatg gtggtgtgaa ctgtacactg aatgtgatat
    27601 tgctaccgct ggacgggaac tgcggattgt tgacagaact tctggacagc gtctccttga
    27661 tgctgatgtt gtgcgagttg ccattctcga tgatgaaacc ttcatactga tcgagctggt
    27721 acagctggta actaaaggtg ctaaccaggt tgaaatagtc atcggggaat ttcagacgaa
    27781 tgccataacc cacagaagca ccgttggtag taaagcttcc ttccagggtg gtgaagtcgg
    27841 catcgtcgtt atcggcgaag aaacttctgt aagccgacag tgacagggag ttcggtttct
    27901 tgccaccaag ccagggctcc acgaatgcga agttataaga ctggtaacgc cttccgttgg
    27961 tgttcacccg gatagagagg gtttgtccat cgccactggg caccggacgc caggcttctt
    28021 tcttgaatac ctgactcatg ctccagttgt tgaacacgat accagcctgc agcaccacac
    28081 caccaaacac acctcctgca taaccggcac tgagttcaag ctgatctgac ggtttctcta
    28141 ctacagtata ttcaatatcg acggtaccat cggccggatt gggaaccgga ttgataccga
    28201 tctgttccgg atcaaaataa cccagcctgg cgatctcact ctgagaacga atgagtgccg
    28261 aacgactgaa tttctgtccc gggtaagtgc gtagttccct ccgcaccacg tgctctttcg
    28321 tcttggtgtt tcctttgatg atgatgttgt tgatggtagc ctgtgctcct tcctggatgc
    28381 ggatctccag gtctatgctg tctcccacca cggccacttc taccggatta agctggaaga
    28441 aaaggtaacc atcgtccata tagagtgagg tcacgtcgcc attcaccggg tcatagcgca
    28501 gtttttgttc cagtaactgc tggttataga tatcgccggg tttgatgcca agaatggaat
    28561 ccaatcgtcc ttcactgtac ttggcatttc cccgccagct gatgtttcgg aaataatatt
    28621 tgttgccctc ttccacatac aggtcgatgc gcaggttgcg gtcgtccacg taatagaccg
    28681 tgtcttccac gatacgggca tcgcgataac ctttggcatt gtaaagtgct atgacctgtt
    28741 tcttatctgc cgataaattt tcaggcatgt atcggctgct gttgaagatg cgcagtgatg
    28801 cacgatcact cacataatcg cggatgctgt ttacagacag gttggagagt atgtaaacgg
    28861 gattgtaatt cggttcagga gcttcgggat cctggtactt gggacgcaac tgtgtcttga
    28921 cccgggtgtc tttcatagta gcccgcagct tacggtcgct gatctcttcg ttgccataaa
    28981 aaacgatgtc gctgatgcgg gttttcggcc ctttgaccac gtctatatac atgaccaccc
    29041 agttcatctg gctggtgtct ggtttggcat agacctctac cgttgtagag aggaaaccct
    29101 tatctgcaaa gtaatctttg atcttggtct caatatcctt gcgcacaaaa tcagtgtaag
    29161 gtgtaccgcg aacgatctcg atcttttcta caatgtcatc ttcttctgcc ttgctcagac
    29221 catcaggaga atacttgctg atgcggggca gctcatccag ctgaatgacc agaaagacct
    29281 gattgcccac gatcttttct gcttcaatgc ggatgttgct aaaaaggcgc agcttccaga
    29341 ggttctgaat ggcacggctg gtttcatcgc cgggaacgtt gatcatctga ccgacaaaca
    29401 aacccgagcg ggccaccagg cttctttcat ccagggtcac aatgccttcc acccggatac
    29461 cggctatctc cagttctttc ggctgggcaa aatctatggc agtggtgtct tttccgaggt
    29521 tggtttgcga catggcgctc aggcccggca acagcagcag catcaaagtg attggtcgca
    29581 gtagtctcat ggtttcattg gtagttgttc gctgatcttt ccgaaacgac gctctctttg
    29641 ttgaaagctg tggatagcca gcagcagttc ttctttatca aaatcaggcc agagggtctc
    29701 tgtaaaatag aactccgcat aggcactttg ccagagcagg tagttgctga tacggtattc
    29761 gccggaagta cggatcacca gttccgggtc gggaatatca actgtataaa gatggctggc
    29821 gatggtttgt tcatctatgg tatccggatc cagtctgcct tcggccactt ctttccccat
    29881 ggcctgcatg gcagcagcca gttcagagcg gccgctgtag ctcagagcca gtaccaggtt
    29941 cagtccggtg ttgccggcgg tcaggctgat agcttcctgc aattcattca cacaatcttc
    30001 cggcagcgaa ccggtattgc ctatggtacg gatgcaaaca ttctggctat tgagctctgg
    30061 tgtctccttt ctgatggtgc gcaccagcag ctccatcagg gcattcactt cttcaatggg
    30121 tcgcttccag ttctctgtgc tgaaggcata gagggtcagg tattccacac ccagttctga
    30181 gcaggcttcc accaccttac ggacggattc aacgccctgg tggtggccat aaatgcgggc
    30241 ccggccgtgt tgcttcgccc agcgcccatt cccatccatg atgatggcaa tgtggcgggg
    30301 aatggaagca tgttgttcag ccgctggttc agacatggtt caaaggtacg aattacggca
    30361 ggctcagaac ttcttgccgc cgcccggcat ggggcatttg aggttgacaa tggaataact
    30421 caggaaagca cctgcataga cgtaggcatc attatgcagg gcatcgcccc gctgacggcc
    30481 accgccgcca ataggctgga tatcggataa ctcggcagaa cgatcagcca gggccaccgc
    30541 gaggtcgccg tttggctgaa tagccagcag ggcaggatcg aaatagaccg agcttacatc
    30601 atcgaggtaa tcggtgaaca gtttatgcca gttcagttcc acaccgaagg taaaattctt
    30661 gctcagggcc accttgaaac caccccctaa cgggacagcc acctgatagc gataataggg
    30721 atccaggcca cttagttcct ctgcattctg accttcggta cccaggggct ggaggtctat
    30781 ccactgccca tcataataag cctgggggtt gaaatgatag agtgcgaggc cggcaaatac
    30841 gaatgggctg aaccattcat tctttttggt gcggctcagg gaaaagaaat tgagctccat
    30901 gcggctggcc acttcccaca gaccggtacg gtagctcagg ttgcgggtct gctggaacag
    30961 atcgtcgctg atggcatcct ctccataggt ggaaccgaaa gctccgccga tgtaccaggc
    31021 caggcgggta ttgaaattat gcctgtagaa catgccaaag gccggttctg caaactggat
    31081 gcttttacgg gagttattga tgtctccaaa agaatggccc atacctcccc agaaaccaat
    31141 ttcgtgttgc tgggcagagg cggtcagcgg catggccaga agccatccca aacccgtgat
    31201 acatatggct ttgagtactt tcaaaagagg agcaaagtta cgtaaccgaa gggatataac
    31261 gggggtgaaa gggggatatt gtcagttggc agtgggcagt tggcagttaa tacaatgtag
    31321 cgtcctgaat acggttgaca gttttgtaaa gttagattag ttgtttgttt tgaagcttta
    31381 cctgacactc aaaggcaaaa ggtgtgactt tgcctagtga ttggtgtggt ttttcttgat
    31441 tatacattct tacagctttt gcaagatatg atttaagttg accatagctg gtaggattat
    31501 agggttttat atagttattc ttgatcgtgc cgtttaatct ttctgcgtgc gggttctcat
    31561 aggctgtttt ccccatactg tttagaaagc gcccttttgt taatgttaga aaggcttttg
    31621 agtagtattg tccgcctcca tcagagtgga gaataggtgt ctgatgtttg tatacatgcc
    31681 tcattgccat cttaagtgcc ggaatggttg tgtgttcgca cttcagagtt tggctggctg
    31741 catacccttt tatcaccctg gagtacagat ccatgatcaa ggtcaggtaa tagaatctgg
    31801 agcctagttc atagtatgta atatcactga cccatacctg gttgacatct gtcaactgca
    31861 gctcgtttat cagattatca aatcgtacca ctccgctgct gtcagttgta cggcgccagt
    31921 ttttctgagg gtgcactgaa taaccgtttt cctgatacca gatcagaaat ttgtctcgtc
    31981 ccatgtgctc agggcacaga cgtgtataca gggatttggc gcccattctt ggatgatccc
    32041 gacgcactgc atccaccata taccttaatg actgggtagc ctgccattcc gcttctgcct
    32101 tttgcaggcg ctggtagaca ttttgtctgc tcgtgcctat ggctctgcaa aactgactca
    32161 ttgtccaaca gtattggtct tgtctggaga taaaccatct gacagtttcg acccaagttt
    32221 ttttttaata tcgacctggt attgctcttc cgccagatcg atcatcttgt ccttaaactc
    32281 aagttctatc tgtttttggc caatgatgcg ttctaattcc cgaatgcggg cctgaagcgc
    32341 atctattttg ctgctatcgc tcataggctc tactattaac cgggcttttc gcccgttgtt
    32401 taccgagtat ttatctaacc acttgtaaac agctgttctt gaaaccccat gctgtcgact
    32461 cacctggtta acagtcgtta atttacattc tatttctttc accttatcct gcttaaagga
    32521 ctctgaaaag tgacgtgact gttgacgatg ccactgaggg cgtgttcctt tggtttgact
    32581 cattctttta agtttgttaa cctaaaatct tgtcaaccta tttcagtacc cgacacaagg
    32641 cggaggtcgg aatatgtgag cagtgaaccc tgtcggaggc agggagtgag ttgtgaggag
    32701 cgtaagtggg agtttgaggt ggtattgtca gttggcagtt aatacaaggc ggaatacgga
    32761 atggtgatgt gtaaaagaga aaatagcgga gtgagtacct gtttgagtgg agagtgactt
    32821 gccaactgag aaccgccccc gcaggggtta actgccaact agttcctttt atcatacccc
    32881 aaccccagct tgttgcgcag ggtatcgagg aagttggttt cgtggaggcg gaggagggtg
    32941 atctcgaagt gttctttgcg cactgccagc tgcacgttgc tgccgatggt ctcgtggcgg
    33001 ctgtcaagcg atgccaggaa tttctcgctg cgcccttcaa tctcgaagct gatgactgaa
    33061 tcgtcgccca ccacgatggg gcgaatattg aggttgtgcg gagcgatggg ggtgatgaca
    33121 aaattggcgg aagtagggaa gatgatgggg ccgccgcagc tcagggaata accggtggat
    33181 ccggtcgggg tactcaccat gagtccgtca gcccagtagc tcatcatgaa ctcgccgttg
    33241 atataaacgt ggatggtgat catggccgat gaatcctttt tgtggatggt gaattcattc
    33301 agcccgtatg gatgcccgtc aaacaggggc acgttcgcat ccacgtggat caatgtgcgg
    33361 ggatcaggtg tatagctgcg acgttccagc gcatcgatgc aggcacggat ctcatccttg
    33421 ctgatgctgg ccagaaaacc cagccggccg atattgattc ccagaacagg aatattgctg
    33481 tctttgatca gggtgaccgt atccagcagc gtaccatcgc cgcccaggct gagcatgtag
    33541 tctgtatcct gcaatgcctt ctgatctgag gcactaaaca gtgcaggctt ctcagaaaat
    33601 ttgatgttgt tgatcaggtg cgggtaataa gcctcgtaga tatggaagtc aataccccgc
    33661 tgctgcatga ggtcaaataa ctcctgcaca taggggatgg cttccttgtg gatcaaccta
    33721 ccatagactg caattttcat aggtattgag gtctagtccg aggtgtaaat atagcccgaa
    33781 ttctcccagg gcattgacgg caccttcaaa gcggaacacc cagtcgtaat aggtggtgat
    33841 gtccatcccg atgccggcag aatacaggag tgtgttgcgc agggtgttgg tactggtgaa
    33901 gacatcgtca gacacataac cggcatcggc aaagaccttg ggatagacct gtaccggaat
    33961 ggtaccgaac tgcgagttcc ggaatatggg cgaatgcagg gtcttgtcta tcgctttcac
    34021 ccgaaggttg ctccgaaaca ggccgatgcc ctgaccatcc actacatagt attcataacc
    34081 ccgcacgaaa ttctcacagt aacccaggct ttcggtcaga aagtagggat attctgccga
    34141 gagattctgg ctgccataca acatggccgc cgcataccac ttaccggtca ggtgtcggta
    34201 gatgttccat tgtccgctca cgaatatctg atcggcagtg ttgttatccg gatcatcaga
    34261 acgaaagaag ccaaagcctc ttttctgcag gatggcccgc aggtaattgc cttccagcgg
    34321 gtagcttctg ttgtccaccc ggttgctgat gaaagtataa cccagggtca tgaattgcag
    34381 tcggtgggct ccgttgccca ggtattccgg gttcaggctg tccatggcac ctgatacacg
    34441 tgtgtcgttg tatttgatct ctaccgtatg cagttcataa ggaccttgcc ggtgacgcac
    34501 ataggtgccc agcttgatgc gtttgcgaag aaaatcttct gtttccatga acacctgctg
    34561 atcgtattga gtattcacat atatctgtct gtttgctgca tacatggcaa acaagcccca
    34621 tcctgtttca gaatctttag acagaaatgg tctttcatat tccagttcca tcttctgact
    34681 aaagccgaag atggcacgca cattcaggcg atctgcatgt ccggtcaggt tgtagtgcac
    34741 catgtccagt ccgtattcca ggcgactcag gtcgtggttc tgctcaaacc accagtagtt
    34801 gatgtttcgg tctgctatat cgatccaggg tgcagggaaa aggaaccatc gttcatccac
    34861 ctgcacctga atggtagcct gtctgtcagt gttgttgaac agggtacaat cagccttgag
    34921 gaaaagacct gtattcagga tgttctcctt cgaccgttcc agtaaactat cgaccgaaga
    34981 ggcagtgatg gtatcgcccg gatgcaggag catctgtttg gtgatgatat aggctttggt
    35041 gtggtcattg ccgcttacca caatggtatc gaccaccagt aaatcttcat tctgtgacca
    35101 tccccgcaag ggaagacaca gcacgcagat catagtaacg aacaaccatt tcggcacgtc
    35161 agatattcag gtagttcatc aatgaatcaa aacggtcttt gtagatctcg ccgaggtcgg
    35221 tctcttcgaa ataggccttc acttcatatt caaatcgctc gaaagtggcg atgatatgcc
    35281 ggatatcggt cttgtcaacc ttgatggtaa cggtgtggct gtcttcgccg tgctggtgta
    35341 caaagctgct gagaatgtgg gcatcattgc tttctacgat gcgggcaata tcactcagca
    35401 cataatcttt tttgttgccc agttccagca cgatgacacc acctgctccg ctgatggagg
    35461 tcatcgcagc gaagtggtag agcaggctct gcagggtgat catacccagg tagtgcatct
    35521 cttcatccac caccggaatg accggagagt tgagtcctgc agctacccgc agtacctcaa
    35581 agatgtgctc actggctttg acataaggct tgtagaggtt cttcaggtag gcaccaagtt
    35641 tttcggtggt atcgttgaag tcgagtatgt cttcttcaga caccagtccg atgtacaccc
    35701 ggttgtcaac cagcggcagg tgagagagtt tgaactccat catccagtcg atggccttct
    35761 caccggtgtc gctgcttttg agcggcggaa tattttcggt gatgatgtct ttggccagca
    35821 ctttatttca gttgatccag aaattctctt aaataacgat tgaactgctc cggttgttcc
    35881 atcatgggag cgtgtccgca ttcgtcaata aaaagcagtt tcgaattcgg gatgagttta
    35941 tggaattctt cgcctacaaa aggaggtgtg atggtatcgt tatttcccca gatcaacagt
    36001 gtaggagctg tgatggaagt gatctcatct gccaggttct cacggatggc gcttttggcg
    36061 gtagccagca cacggatcac cttgttgcgg tcattaacga tgtcaaagag ttcatccacc
    36121 aactcttctg tggcatgttt cgggttgtaa aaagtgagtt cagccttgtt ctgtatatac
    36181 tcccgatcac cccttttagg ataggaatca cccattgcac tttcaaacaa tccggagctg
    36241 cctgtcaggg tgatggagct gatcttgtct ttgttatcaa gagcatacag cagtgcaata
    36301 tgaccaccca gtgagttgcc cagtacatgc accttgtcat aaccccggaa ggctacaaaa
    36361 cgctctacat actgcacgaa tcctcgcaca gtggtctctt caatgggcag ttcatacaag
    36421 ggtaatattg gaatgaccac cttgtaggaa tccttgaatg cttcgatgat gtcgtagaaa
    36481 ttactcaggg cgccgaagag tccgtgcaac agcatcagaa cctcgccctc accttcttcc
    36541 acgtaacgga aaccgttctc ctctgtaaat gtatgctcca tagccttcag ggtcggttac
    36601 aaatataaac cttttgattg ccttagtatc agcggtacaa gttacgccag ttgtgcagca
    36661 gtgtcagtcc gtcaggtgtg aggatgctct ccggatggaa ctgcacaccc caaaggggaa
    36721 gctccctgtg ccgcagtgcc attggttccg cacgatctgt cacggcagtg atctccagtt
    36781 gagcgggcac atcagcaata ttcaggctgt ggtatcgcat aacctgcatg gtgtcaggaa
    36841 gtccggcaaa cagatcctgc ccggagtggc tgatcatgct tgttttgcca tgaacaggta
    36901 caggtgaacg ttccagcctt gctccgaaat acataccaat tgcctggtag ccgagacaga
    36961 ccccaagcat gggaacggat gcatgaaacc gatcgatcac ctgcatggtg atgcctgcct
    37021 gcaggggtgt ttccggtccg ggactgatca cgatggcttc cggttgcaaa gccccgatct
    37081 cattcagtgt gatggcatca ttccgatgta tggttacatc tgcgccggtc tgacagagat
    37141 ggtcgtacag gttccaggta aagctgtcat aattatcgag cagcaggatc atacttccat
    37201 ggtttccgga accggcagga cgccgttctg cttatcgaac cagtcctgca tgtgctcaaa
    37261 gacatcgcga acaagaggaa cggcatgccc gatcagatcc attgctgcgg gtgcaaagcc
    37321 caccagtggt tcatagacct ttgaagcagt tttcagttct tctgtgaaca cacccatatt
    37381 attgccatac cacaggatac tgctgtacac cagggtccag ctcagcatcc acacgaaagc
    37441 acctgccagc ttgttcagaa agttgagttg caatgcctgg aaaacacctt ctaccaaacg
    37501 acccagcagc ctgaagacca atccaatgag gaacatggtt accagaaaag agatgaaggg
    37561 catatagtct gcatgcagat ggaaccattt ttccagatac gttgctgtga tatggctgca
    37621 ggatactgct gccgcaattc ccaccagaac agacagcaac cagaagagcg aaaagatgag
    37681 ccccctgctg tagccccgga acaaggccac cagtagtact ccggcataga aaacgtccag
    37741 gatcatcctg ccagcaggtt gcgcaccatg gcagagatgg ccttaccatc tgctttgccg
    37801 gccagtgact tggttgccac acccatcact ttacccagat cgccgggacc ggatgcgccg
    37861 gtttcggcga tgatcttctc cagtgctgtt ttcaattcat cttcgctcat ttgagccgga
    37921 aggtagcgtt caattacagc gatctctgca gcttcatcag cggccagatc agccctgttc
    37981 tgggcagtgt aaatatccag gctttcctta cgttgtttga ccagtttggt cagcaactgc
    38041 atttcctttt cagcactgag ttcatcactg gcaccttttt cggtctgggc aagcaggatt
    38101 gctgatttga tgccacgcaa agcacgcaga gtagcctctt ccttgctttt catagcttgt
    38161 acaatgtctt tattgacggt atcgagcagc gacatagtta gtgatttgac tggttcaaaa
    38221 gtaggaagaa aaaagggcgg aatacggaag ggtaagaaag aatggtttgc cggtattatt
    38281 tccccttgcg gccgaaggtg cggatataat caaccaggtt gggatcttcc agtccttcca
    38341 tatcactgag ctccagttcc agcgcacggg tgctgcgcag tatctcaatg agactgatga
    38401 ccagactggc actgaacagg atcatggctg cagcaaaaca ggcattggcc cagctcatat
    38461 attctatgta aataaaatac atacttagta aggccaggat gaaactcagc acccccagga
    38521 actgcatgcg cctgaccagt ttcagtcgga aacgcaggtt gttgatctgt tctttcagaa
    38581 acgggcttgt ttcgtttcta aggtactttt catgcaggtt tcgaaccaca ctcgataagg
    38641 caagaaaacg gttggtataa gccagcatga tcaggctgat ggccggaaac aacagggcag
    38701 gtgtattgat ggtgatgctc atactcaggc ggtagcagat gcctgttcca gcacccggat
    38761 gtagtaggcc tcatagagtg gcatgatcct gtcaagagag aagcgctgag cttgtttgaa
    38821 agccctttcc ttaaacgctg ccagggtctc ttcattttcc aggatacgaa tggcattggc
    38881 ggccatatca tctacatcgc caacaggact tagaaagccg gtttcaccgt gaatattgat
    38941 ctccggaagc cctcctgtat tggaagatat cacaggcacc tggcatgcca tagcttccaa
    39001 agctgcaagc ccgaagcttt ctgtttcaga cggaaggatg aagaggtctg agatggccag
    39061 cagttcttca atggcatcct gttttcccag aaagtgaaca tcatcctggt ggcaaacctg
    39121 tctgctcatc ttttcgatgt tctggcgttc cgggccatca cccaccagca atagtttagc
    39181 gggcatttcc tgcctgatct tgcaaaacac ccgcactaca tcatctactc ttttcacctt
    39241 tcggaagttg gaggtgtgca tgatgatctt ttcgccattg cttgcaatga ccttgcggaa
    39301 atggtcttta tccagttttt taaacctact gaagtcaata aaattcggaa tgacctcgat
    39361 gtcttttcgg atatcgaaat gttcgtatgt ggtgtctttg agactctgag aaacggtagt
    39421 gatgccatca ctcttattga tggaaaaggt gaccaccgga acgaaggtct catctttgcc
    39481 caccagggtg atatctgttc cgtgcagggt ggtaatgaag ggcagatggt accctttttc
    39541 tttcaggatc tgtttggcca gataggctgc actggcatgc ggaatggcat agtgcacatg
    39601 cagcagttcc agttgttcgt actcggcaat atcaacgatc ttacttgcca gggcggtttc
    39661 ataaggggga taatcgaaca gcggatagtt gctgaaattc acctcatggt aaaatatgtt
    39721 ctcatgaaag gaatcaagac gaaatggctg cttgtaggta atgaaatgca cctgatggcc
    39781 tttatctgca agggccttgc ccaactctgt ggccactacg ccacttccac cataggtggg
    39841 ataacacacg atcccgatct tcatacgctg caaacttatg ttacttaatg cattaatgag
    39901 acgaatggtt cacctccatt attgcaaacg atgtcaggta atgaccctga tcacagcaca
    39961 tcctgtcact ttctgtacag atcctgcatg gctgcggtca ggtcaggaaa acggaaacgg
    40021 taaccggtat ccagaatttt ttgcgggctg acacgctggc tggccagtac cgctgtgctc
    40081 atctcaccca ccagcaggcg aagaacaaag ggtggtgcag gtgccgggat actgaagcct
    40141 cctctggctg cagacatagc acccaatatc gttttctgat cctgaatgcc gggagccaca
    40201 gcattgaaga tacctgatag ttcctgtttg accgagtgct gaaaaattcc tgccacatct
    40261 tccaggtgta tccatggcca ccactgtttt cctgcagcga aaataggtct gataccaaaa
    40321 cgcaggtagt tgtccatctc ctgccacaca cctgaacctc gacccagcac cagacctatg
    40381 cgcatgatag ccacaggcag tcctgtttct tccaatccat gagcagcctc ttcccaggtt
    40441 tgaacagaag tgctcagaaa acctgcacca ggagtggagg tttcatccag cagctcatgg
    40501 cctctggtgc catagaaccc gacagctgag gcactcacta ttgatctgac agtatgtgat
    40561 cgggaaagga gcgcctcttt cagcaatgaa ataccctgta ccctgctgtc tatgatcagt
    40621 tgtttgcgtt gatctgtcca tcgcttgtca gcaatgcctt cgcccgaaag gatgaccagc
    40681 cgggtgatgc catcaaaggc attttcgccc aggaatttcc gggaaggatc ccaggcgatc
    40741 ctgttcaccg caccttcctg tccgctcttc cggctcagcc aggatacctg atgaccagac
    40801 tcctgtaaca atgatgtaag tctttgtcct acaaatccac tgccaccggc tatcagtata
    40861 tgatctttca ttttcaggtc tggttccgtt gcagaaacaa cccgggattg gtttggttgc
    40921 cccacaccgc ttaaaaatcg tacttttaaa actctaaaac agccatatgc ttcgcccaac
    40981 cctgttctta agtgttgtga tcacgctgcc tttcctgttt tcctgcaagg gaaacaagac
    41041 ggcttctgaa catgctgata ttattggtat tgacagcagc agatacatcc gcattcctga
    41101 tgccgacagt ttacttcccg gctggcgaac agacaattct gtggtcaatc attggatagg
    41161 cgaccctgat aacctgcacc ctaccaacgg acggaccgcc tcgcgttcct ggattctcag
    41221 ccttacacat aattacctga tgaggctcga tccggtcagc atggaggttg tgcccgatct
    41281 tgcaacagcc cagcctgaaa tcagtgccaa caaactggaa ttcacgtatt ccattcgtcc
    41341 ggatgcccga tgggatgatg gcaaacctgt tacagcggca gatgtagcct ttacattaaa
    41401 agtaaataaa tgtccgctga ccgataaccc caatgcacgg tcagagactg aaaacctcat
    41461 ggatattcgt ttgtatccgg atgacccgca aaagctcacc ctgatcatgc accatgaata
    41521 ccttttaaac atagcatttc tggcaacctt cgctatcatg gaacgcgact ttcatgatcg
    41581 tgatcaggtg atggatggtg tcagttttgc acaactcgaa gatcccgcct ggttggagaa
    41641 tcctcctgct gatgtagtgg catttatgca ggagttcaat gacggcaaat acgggaatga
    41701 cccgtcctta tacaatggaa gcggaccata taaagtggtc acctggaaca ggcatgcaga
    41761 tctgatatta gataagaaac caaatcactg gaccgatcag atcaaagacc caaaagtttt
    41821 tcaacaggca aaaaatgacc gtatcgtatt tctggagatc aaagatgaca atgcacagat
    41881 gcttgagctc aaatctcaga ccatagacgc tactgtctgg ctttctaccc aaagtatgct
    41941 ggcattagaa gcagatgaga atttcaaacg caactacaac tatcgctata ctgataatta
    42001 cagtttcaat tacctgggtg tcaacacaca gccggacggt atccggcacc ccaaaatatt
    42061 tgacagcaaa gaggtgagac aggccatgac ctatctggtt ccaatagaag atatcgtgca
    42121 tgttatttac aacgacaggg ccactgtttg gccggctttc ctgtcgccgt ataagaacgg
    42181 gttcaacgac acactaaagc cttactacca tgatctgaac aaggctgtta gcttactgga
    42241 aaatgccgga tggactgata ccgatggcga cggaattcgg gataaaatgg tagatggaaa
    42301 aaagacagat atgcgttttg aactcttgta cagtcatgca ggtgcctgga tagatcagat
    42361 ggcaggcatg atcagggaag catttttagc cggaggaatt gatgtgaaag taatggtaat
    42421 ggatcttcct gctttatcac aaaaagtaag ttcgcatgac tttgatgctt acctgggtgc
    42481 ctggggaacc aatagcctgc cggacgatta taccgccttg tggcatacct cctcctatgc
    42541 cggtggcgga ccgaatttct gtggctttgg caatacctat acggatgcat tgatagacag
    42601 tatgaacctg gtggtggatg agtctgtaag ggcacccatg atcagagagc tacaggccat
    42661 tgttcatgat gaggcacctt atatcatgta cctgtcagca agcagaaaga atgtcatcca
    42721 taaaagactg ggcaaccaga tcatgacctt tgaaagacct ggcaatatgg tcaattacct
    42781 gcaggtcatc gggcatggaa tgcaggaatt gcaataaccg gtcagcgcta tgagcaaagt
    42841 tctcctgcgc cgtctgttat tttttctgcc cacgctggtg gtcatttcgt tgctgtcatt
    42901 tcttatcagt atttctgctc ccggcgatcc tgttgacaga ctgcttccca ccgccggtac
    42961 cagtggagaa accgggcttg tatcaaaacg catcatggaa gaacgtcagg ctatgcgcca
    43021 tcgtttaggt ctggatctgc ctgcgtttta tctgtctgtt tcttctctga gtatggcagc
    43081 tgagatgccg gattttcaga atccggagga agaa
//
LOCUS       DAOMAX010000003        34220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_147_length_34220_cov_4.966457, whole genome shotgun sequence.
ACCESSION   DAOMAX010000003 DAOMAX010000000
VERSION     DAOMAX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 34220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34220
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147_length_34220_cov_4.966457"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1913
                     /locus_tag="PK742_00435"
     CDS             75..1913
                     /locus_tag="PK742_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU75082.1"
                     /translation="MKKIFITLLIFIFYSVADAQMQIYPPHWWTGMQDSTVQLMVHRE
                     NVAATTPTVDHPGVRLDSVIRTENPNYLFLNLTISENARPGIMTLRFALRGRPEVAYY
                     ELKQRAERSLAYGLDGNDFIYLIMPDRFVNGDPSNDIVPGTQETTIDRSVINARHGGD
                     LQGIIGKLDYLQDLGVTATWLTPPQENDQPKYSYHGYAMTDHYDIDPRIGDLSLYRQY
                     VQESHARGMQVVFDVVHNHIGNEHWLFTDAPSANWYNQWDSFTRTNYRTSSLMDMYAS
                     EEDRRLMTDGWFDTHMPDLNQRNPLVAKYLTQWTIWWIEEMQVDAIRLDTYSYADQTF
                     LSQWALDVLQEYPGFTLFAEVWVNGLGVQGYYNGNNNLNRNVNTHLSGVLDFEMNWTL
                     GKLVNEDPGWNSGNGALYLYLCQDYLFGENGGADNVIFLDNHDMSRFYEVTGKDLDKM
                     KMATGILLTTRGIPQWFYGSEILMDEPADPMDRVREDFPGGWPGDTLNKFTAADRSPE
                     ENTFFQYLRTLARWHAQEPALKNGKLMQFVPDNGIYVYFRFTDDASVMVIVNGNAEEQ
                     ELDTARFRERLHGYTSAVNIESGALLKSLEVLQLPAMSTTILKLHP"
     gene            1910..2494
                     /locus_tag="PK742_00440"
     CDS             1910..2494
                     /locus_tag="PK742_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQU75083.1"
                     /translation="MTGFEQLDAHIARFINLDDAQRQYIHGLLEQREVRKKDHLFRAG
                     QICRYEYYVVRGCLRVYFLDDHGSEVNLMFPVEDWWLGDLISFTEQTPSALYVEALED
                     SQLLMINKENKESLYEKIPAFERMFRVLILRNLAVLQHRLIATISHSAEERYLAFIER
                     YPDIPQRIPQHHIASYLGVSPEFLSKIRKKLSAQ"
     gene            2519..3598
                     /gene="corA"
                     /locus_tag="PK742_00445"
     CDS             2519..3598
                     /gene="corA"
                     /locus_tag="PK742_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HQU75084.1"
                     /translation="MALRKRNKSAKNVKKKTGQSPGSLIYTGVQHLDKVHIEYIEYNE
                     VDLVQRDHVSLEDLEACRNSDKISWINITGLHDTELIGKVGEIFGLHTLLLEDILNVN
                     HRPGYHEDEQVVGAFVKMLTPDPGDPFSQTEQVAILAGSGFVLTFQEYPGDVFGSVRE
                     RLLRPTTRIRSRKADYLAYALLDAVVDQYLVVIDELGVRVEDLEDSMMQVFRKSLLDD
                     IYMYKSDINFLRKIIRPVREIALQLEKSEAEFVEDATGPFLKDLVDHAEYAAESVETF
                     RETINDLLNFYHTQSANRLNDILKILTIFSVVFIPLSFLTGLYGTNFEFIPEIHREHA
                     WPVFLGAEAVIAIVMILFFKSRKWL"
     gene            complement(3599..4279)
                     /locus_tag="PK742_00450"
     CDS             complement(3599..4279)
                     /locus_tag="PK742_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQU75085.1"
                     /translation="MKHTVGVFIFCLFLACTLSEDPELPVPAHCSQVLLVETETELDS
                     LATLFCLERTGRQWKILDSMTVMVGRKGLAADPVQLPMGYSRTKQEGDKCSPAGSYAL
                     LFAFGYAPDADTRMPYRQMTDEDICVDDSLSGAYNLPSKRTVASYLSAEDMRRSDDLY
                     SKGIWVGYNTDPVMPGYGSCIFMHIWKDQGYPTAGCTAMSAEDMQQLFGWLDPVANPM
                     LVQRIRGH"
     gene            4319..5380
                     /locus_tag="PK742_00455"
     CDS             4319..5380
                     /locus_tag="PK742_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75086.1"
                     /translation="MKRIYILLSAFFLFSAAHAQITITVDDLATEGDVIYFSNATPLG
                     EFDLTADGPDQVWDYSWLDQTGQDSSVWVDPFETNPAYFLLWFGSSVAEETGQTFSSD
                     FFSLEDIYNFYDRNEGELSITGIAGTVTGIPLPASFDDADVLYELPLNYNDSYSSASG
                     FAFSLPGIGSWSESRDRSCLVDGYGEVTTPYGTFDALRVNCELLVTDVVEYSGIEFPI
                     SYTTREYRWMAPGMKIPVLFISSQEILGFETVTNIQYIDSLDGAPVGIQLPTAVPEIL
                     VTPTLVQQGLCQLQLGDPVGADVIITDLQGKQVLENIHVAPGQSVSALPVGGLHAGWY
                     WCMVKQGEHLVSRPFLIAE"
     gene            5592..6368
                     /locus_tag="PK742_00460"
     CDS             5592..6368
                     /locus_tag="PK742_00460"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQU75087.1"
                     /translation="MSSILLGSALLVLVFVSVVWILSVLAKDMSIVDIAWGLGFAALV
                     IFQWVRWAPASPRSFLVGYLILIWGLRLAVFLAIRNLRKGEDPRYAAWRHDWGKNTWW
                     ISYFKVFLLQGALMLIISMPLHVTTAAGKSMGSWTDFAGTLLWLGGFLFESIADWQLY
                     RFKKDPANKGMVMDKGLWKYSRHPNYFGESLIWWGIFLVAFGAGTAYLSVIGPVLITF
                     LLLRVSGVTLLEQRYEGNDKYADYKRRTSAFIPRPPKRQV"
     gene            6365..6937
                     /gene="nadD"
                     /locus_tag="PK742_00465"
     CDS             6365..6937
                     /gene="nadD"
                     /locus_tag="PK742_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HQU75088.1"
                     /translation="MKIGLFFGSFNPIHIGHLIIAQHFADWTDLDQVWLIVSPQNPFK
                     EKSGLLDEKHRLYMANIAVEDNDRIRASNVEFHLPQPSYTIDTLTYLEEKYPDHRFVL
                     LMGSDNLPTLHKWKNAGLLLQRYPIRVYLRPGQSNTNPYPEADITFTEAPLLNISATF
                     IRQAVREGRDIRYLVPEKVYEYIRSMHFYQ"
     gene            7325..8320
                     /gene="nadA"
                     /locus_tag="PK742_00470"
     CDS             7325..8320
                     /gene="nadA"
                     /locus_tag="PK742_00470"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQU75089.1"
                     /translation="MEIVETGWETKGYLDIPVDPTLDLVREIDKLKREKNAVLLAHYY
                     QDSDIQDVADYIGDSLGLAQQAARTKADIIVFAGVHFMAETAKILNPQKKVLMPDAHA
                     GCSLADSCPPPLFRKFKEQYPDHTVISYINCTAGIKALSDIICTSSNAVKIVETLPPD
                     TPVIFAPDRNLGRYVMEKTGRDMVLWNGTCMVHEIFSQEKITKLRHRHPDAKLIAHPE
                     CEEAILEMADFIGSTTGLLKFTREDNSPVYIVATETGILHQMQKASPHKQFIPAPPDN
                     LCACNDCPHMKRNTLEKVYTCMRYEQPEILMDKTILEQARKPIQRMLDISASLGL"
     gene            complement(8327..8644)
                     /locus_tag="PK742_00475"
     CDS             complement(8327..8644)
                     /locus_tag="PK742_00475"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQU75090.1"
                     /translation="MAKTTDQKAFLSVDISKLEKSAEIMRALAHPLRMKIMGFIDKNK
                     NVHVNKIYNTLGLEQSITSQHLRILRNADLVKTKREGKFIFYSLNYDKIAKVLSMMEK
                     YDV"
     gene            complement(8669..9001)
                     /locus_tag="PK742_00480"
     CDS             complement(8669..9001)
                     /locus_tag="PK742_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQU75091.1"
                     /translation="MASTPSIKLANAPKGNIKLEYKNLRKTVLILRAVNHKLRLKIIK
                     MLTDKEKMSVTEIYVKLRLEQSVASQHLAIMRRAGVVTTTRSGKNIFYQLDNDRLKSI
                     GNFIRDIS"
     gene            complement(9105..9782)
                     /gene="tsaB"
                     /locus_tag="PK742_00485"
     CDS             complement(9105..9782)
                     /gene="tsaB"
                     /locus_tag="PK742_00485"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQU75092.1"
                     /translation="MATILQIESSHPLSSVSLAADGNTLLEIRGEEAQNHASQLGPMV
                     QSLLDRAGFSIQSLDAVAISIGPGSYTGLRIGLSLAKGICYGSTIPLIPVSTLACMTA
                     CAINQYPMQETLYCPMIDARRMEVYTALFDHTGTLLCEPEAYILDNKPFREQTEQRQT
                     CFFGSGSNKAVHLLEDKNSKILTNFIILSSGMNKIALDAYNKGIHEDPAYLEPVYLKE
                     FYHTNGK"
     gene            complement(9783..11423)
                     /locus_tag="PK742_00490"
     CDS             complement(9783..11423)
                     /locus_tag="PK742_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQU75093.1"
                     /translation="MRSFILSGLLFLTGFVQGQNAADQIKETVEFLSDDRLEGRATGS
                     RGEEIAANYIAGKFSALGLQPAGENSGFFQVFSFSTGKEAGEQNFVVANSKRADAQPV
                     FFSGSGQVQAQVAYAGYGITAPELQHDDYQGLEAKGKILLIQLSTPEGTHPHTDFYPY
                     ADERSKVELAMEHGAVAVLFYNMDQDYDPSLLLDYSSRAEAAGIPVLFIEPETANTLT
                     QGLEILVGADIHPVRKEGKNIAARIDNGAPCTIILGAHYDHLGYGESGGSLYRGQPAI
                     HNGADDNASGVALILQLAENLAKDPDDHYNYLFIAFSGEELGLYGSKSIADFLTAQDD
                     CYNYMLNFDMVGRLDSIKTLIVNGYGTSPAWTRIDDLGKMQGFTIKTYSSGIGPSDHT
                     SFYLKDIPVLHFFTGTHEDYHKPSDDADKINYKGIVELHDLVRALMADLDDEDQVEFT
                     RTKEEESEETPRFTVGLGVIPDYTFEGKGMRIDGVSDGKTAANAGLQTGDIVIRLGDY
                     NVADMMSYMKALSMFEKGDATTATVIRAEEEMTFPIQF"
     gene            complement(11456..13114)
                     /locus_tag="PK742_00495"
     CDS             complement(11456..13114)
                     /locus_tag="PK742_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU75094.1"
                     /translation="MKKTLAIAGVLSSGLALTLLFNSNAPGSDLFEISKNIDIFTSVY
                     KELNTYYVDDLDPNRIMRTGIDAMLESLDPYTNYISEAELEGYKFQITGNYGGIGATI
                     RPVDGKVTVVDVFEGFPAFKAGLQPGDELKKIDDHTVLENDESSISELLRGSPGTPVA
                     LTVFRAGTGETLQLELTREEINQGNVPYFGMVDDHTGYIMLTQFTEDAGRNVADALKK
                     LQEDHPDLSGVILDLRGNPGGLLREAVNVSNVFIPKGLEVVSTRGRVEEWDKSFVSIN
                     NPVDTEIPLVVLTSRSSASASEIVAGVIQDYDRGVILGQKTFGKGLVQTTRDVSYKTK
                     LKLTTAKYYIPSGRCIQAIDYANRNEDGSVGKIPDSLKVAFSTRAGRTVYDGGGIDPD
                     VAVGEENYRDITISLLQGNHIFHYATQYFYSHPNISPANSFSLTDEEYDGFVRFMSGR
                     TYEYTTETEELLAAFEESAREDAYYDAVKNEIDGLHAKVTHDKQNDLYKFKDEILFEL
                     NREIAGRYYNYNGRVEVSFRYDPDIQEAIRTLKNPEAYTKLLSK"
     gene            complement(13126..13350)
                     /gene="yidD"
                     /locus_tag="PK742_00500"
     CDS             complement(13126..13350)
                     /gene="yidD"
                     /locus_tag="PK742_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HQU75095.1"
                     /translation="MVSKWVSGLLVLLVRFYQVSLSPLFPNSCRYSPTCSQYMIEALR
                     KYGPLKGGWLGLKRLSRCHPWGGHGHDPVP"
     gene            complement(13340..13729)
                     /gene="rnpA"
                     /locus_tag="PK742_00505"
     CDS             complement(13340..13729)
                     /gene="rnpA"
                     /locus_tag="PK742_00505"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQU75096.1"
                     /translation="MPDPAYDLPLTHRLKSRAAISLLFTEGKSLVQEPLRLVYRQQPA
                     KGDVAYRIAFSVSKRNHPKATDRNRIKRLLREGFRLEQHQLQIPEGIHADIICLYQSK
                     KPTTFNELKGKFHLALERLSKELERGL"
     gene            complement(13748..13915)
                     /gene="rpmH"
                     /locus_tag="PK742_00510"
     CDS             complement(13748..13915)
                     /gene="rpmH"
                     /locus_tag="PK742_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQU75097.1"
                     /translation="MKPTFQPSKRKRVNKHGFRSRMKTKDGRAVIARRRRKGRKKLTV
                     SDERTMMRFRV"
     gene            complement(13997..15532)
                     /gene="rho"
                     /locus_tag="PK742_00515"
     CDS             complement(13997..15532)
                     /gene="rho"
                     /locus_tag="PK742_00515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQU75098.1"
                     /translation="MYDILQLNEMLVPELKEIAEKLQVKDIRKLNKQDLIYKILDAQA
                     VAPQEIAENGQEETTQKAEKKDKKRNMRPRKKKEDGAEEPEKAEIPVVAEETPVKQEA
                     PRKEEAVRETDTVRPVEENRQENRHQYPQKRRERFNIDLEGAIPGEGVLEMMPDGYGF
                     LRSADYSYLSSPDDVYVSPSQIKLFGLKTGDWVKGTIRPPKEGEKYFALLKVETINGR
                     DPKEVRDRVPFDYLTPLFPNEKLNLVYSPTDYSTRVMDLFTPIGKGQRGMIVAQPKTG
                     KTMLLKSVANAIAHNHPEVYLIILLVDERPEEVTDMERSVNAEVIASTFDEPADRHVK
                     VSSIVLQKAKRLVECGQDVVILLDSITRLARAHNTVAPASGKVLSGGVEANALHKPKQ
                     FFGAARNIENGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMELQLDRKLSNKRIYPA
                     IDVTASSTRRDDLLHDRDVLQRMWILRNHLADMNTEESMNFLLQNMKGTKSNEEFLIS
                     MNS"
     gene            15723..16997
                     /gene="serS"
                     /locus_tag="PK742_00520"
     CDS             15723..16997
                     /gene="serS"
                     /locus_tag="PK742_00520"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQU75099.1"
                     /translation="MLEISFLRNNLSEAKKRLAKRPGYDTGVLDELLAADDERRRLQA
                     DLDQVLGEQNRLAKEIGALFGQGKREEAERLKEQVSGLKEQSGDLQKQHDDTADAIRT
                     MLLMLPNLPQSRVPDGRSAEDNVVVKEGGNMPELTDDAQPHWDLAAKYQIIDFELGNK
                     ITGSGFPVYRGKGARIQRALIAYFLDKAQERGYTEYMPPLMVNEDSGYGTGQLPDKEG
                     QMYHMTVDNFYMVPTAEVPLTNIYRDVILKEEQLPVRMTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVHIEHPDRSDETLDEMVSYVASLVESLELPYRILHLCAGDTGFASS
                     CTFDFEVYAAAQKKWLEVSSVSNFESFQANRMKCRFKQAESGKNQLVHTLNGSALALP
                     RILAAILENNQTPEGIRIPEVLRPYCGFDMID"
     gene            complement(17003..17428)
                     /locus_tag="PK742_00525"
     CDS             complement(17003..17428)
                     /locus_tag="PK742_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HQU75100.1"
                     /translation="MRSAFTACFFLLMAVTSKAQYQDAIVGKWLSADGDGKVEIYKKG
                     DKYYGKLVWLKEPNEDNGTPKVDDENPDASKRSTPLMGLVILKNFTFEDGFWQNGTIY
                     DPKNGKTYKCEMWMDGKNTLRIRGYVAFLHRTESWTRSN"
     gene            complement(17449..18450)
                     /locus_tag="PK742_00530"
     CDS             complement(17449..18450)
                     /locus_tag="PK742_00530"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQU75101.1"
                     /translation="MQVAIVGATGVVGQMMRRVLEERNFPVTRLIPVASERSAGKSVR
                     FNDDMIPVVTVEEALTLKPDLALFSAGATTSLAWAPAFEEQGAWVIDNSSAFRMETDC
                     ALVVPEINPDSISGKRQIIANPNCSTIQMVMALAPLHQAYGIRRIVVSTYQSVSGSGA
                     KGLMQLHTERSSNCRLTENPAYPHRIDMNVIPHCDVFLDNDYTREEMKLHHETRKILG
                     SDSIQVSATAVRVPVSGGHSESVNVTFGQDFDLDEVRRLMASMPGVVVLDNPAHAAYP
                     MPLEAEGKDEVFVGRVRRDFTAENTLNLWVVSDNLRKGAATNAVQIAQWLAENGLLH"
     gene            18556..21165
                     /locus_tag="PK742_00535"
     CDS             18556..21165
                     /locus_tag="PK742_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQU75102.1"
                     /translation="MRKAVVGILLILLTGRVFTQSLTDDFSDGEITSDPSWTGDTDQF
                     LVNASQQLQLQGDCDAGGINYLTLAAATSDSVVWEFYTELDFDPSSSNHMRFYLQSDA
                     ADLSAALHGYYIRIGEDGTNDAVKLYRQDGTGNTLLLSGETGAVAAAPAVRVRVIRNA
                     EAVWHMLVDYTGGTSFTDEGSVTDNTYNTGYYTGVYCQYTSTRCDAFYFDDIYISPLY
                     EDDEPPVLLSAAAISDLQVKAIFNEPLQASDAENGSNYTVDGGVGNPIDAALQAGGSS
                     VVLTFADDLPAAVLLHLEVNGIADLAGNVCGLQSQPFTNFSPGLFDLLITEIMADPEP
                     VQELPAAEYIEIWNATPFPVNLEGWTIRDPGTTSQPFPAVVLEAGAYLIVCSAGNTAE
                     FEEYGDVLGVGSFPALNNTGDSLYLFDADEELIHSVYYTDSWYADPIKENGGYSLEMI
                     DTGNPCQEEANWIASIELSGGTPGQENSVAADNPDVVSPSLVSVFPSSATSLYITFDE
                     YILTEALTTTMFAISPDIGSPLSVSADPYVATVIELPLPEALEPGITYTLTCTGLTDC
                     SGNAIGVHNSLEFGLPETPGPGDVIINEVLFNPATGSDDFVEIYNNSNHFIQLNQLRL
                     TERNVENAAEVLEYCYMGEYGRLLAPGAYFFITEKVSWLAPFYDIAPNAQFSENSQCP
                     NFPDDEGIVSLELSSTGVVLDQLHYYDDWHYPLLEDDNGVSLERLRFDAPTQQAENWH
                     SAAEAAGYATPGRLNSVVSSFEGNAGTWTLESEVFSPDGDGYQDLMVLTYTQPAEGTS
                     ASLSVFNLAGQLVAIPARNLYLGVEGLLVWDGLDDSGKQLPTGIYILYVEEFDLTGRT
                     DHFKFPVTLARKQ"
     gene            complement(21210..21656)
                     /locus_tag="PK742_00540"
     CDS             complement(21210..21656)
                     /locus_tag="PK742_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75103.1"
                     /translation="MKRIFAPLVFTLTLLLLLPSCSKQRQLADNLTRQSGRWNIDNYK
                     VTITTTDLPPDVTEYGDSGYMYFFSNGDGQWIDLKDPDEFIFIEFEWDITDDNALQIR
                     YNNEEFNFLFEVNEKDRQVIVYEETVALTEEIDVTTRIEYELLRIE"
     gene            complement(21668..22135)
                     /locus_tag="PK742_00545"
     CDS             complement(21668..22135)
                     /locus_tag="PK742_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75104.1"
                     /translation="MKRSLFILFASLVTLFVACSPEIKLQNHLTRDGGRWNIDEFKVT
                     SVTKTDPPTEPTIIQEFNVGEVLFYESGTGIWIQYDTLTMQNVATYFEWENTNNSITM
                     DLEGFTEEVEFSINEVSKDEQWWVREESFFVGDLETVVTTEIHIVREEEIGNK"
     gene            complement(22256..22852)
                     /locus_tag="PK742_00550"
     CDS             complement(22256..22852)
                     /locus_tag="PK742_00550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQU75105.1"
                     /translation="MSGRHRIYLAAAVTLFGMGGLGLLLMIVFHEQTFSNFFHGEKQP
                     VLLQLLYGALYGIMAVLLLLRLLRLRFLKHTGQFFRKLLLRYRVGWLEIIMISVSAGV
                     GEELLFRGGIQPWLGIWPTALIFVALHGYLNPRDHAVFIYGVVMIPIAAGLGWLYKES
                     GIWAAALAHTIIDVVLMARILTRNQDPYATGKQADPVG"
     gene            complement(22854..23858)
                     /locus_tag="PK742_00555"
     CDS             complement(22854..23858)
                     /locus_tag="PK742_00555"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HQU75106.1"
                     /translation="MHKLLSGSLLLASVLLFSQCKNNNPGTTSDLSIIDTTGLADGEP
                     ITFLIDSSGEKIEAGKDQVIIQFNPPAGKVYNTSMQTITNITQTMFGQTFSNLMDIST
                     SNSIRVMENKPDSNIVLQSQVKSFKAHFKQDSLEASFQNGVSSDDPDIDMIRKIMDCY
                     VDMPVKLTISRETDVVDIGGIEEIQSKINTQLGQEALMASLQMSDMNQDIINSFVAFP
                     HRPIAIGDTWKSTDSVDLGGVPAIVTNTFTLNSFDDKFALVSVQSEFKVDREMVRMMG
                     GTGDEVQMSGYQKGEITVAVASGWSIESKLEQNLNLSMEMQGESMKVSIKGNSTLKTK
                     "
     gene            23908..26991
                     /locus_tag="PK742_00560"
     CDS             23908..26991
                     /locus_tag="PK742_00560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQU75107.1"
                     /translation="MESRTALTEQFKKAYNSLNDAQRTAVDAIEGPVMVIAGPGTGKT
                     QILAVRIGNILLQTDALPENILCLTYTEAGAIAMRNRLLSFIGPDAYRVQIQTFHAFC
                     NEIIQSHPDLFGRRELEPVSELEQVMILENILRELPSDHVLRRFRDDLSSDIRRLGNL
                     FRIMKEEDWSPEWIANAIQAYLDSLPDRPEFIYKVAVTKKGVKAGDVKQDKIDQETAA
                     MEKLQAAANLFPVFQEKMAEAGRFDYSDMILWVVRAFARHPDLLRTYQERFLYVLVDE
                     YQDTNGAQNEILRSLIGYWDVPNVFVVGDDDQSIYEFQGARVQNILDLYRQYENDMRT
                     VLLTENYRSVQPVLDVSRILIDRNEERLVSHLEGLSKVLISRGEEVRDLTQLPVIRAY
                     DSKVQEEAGVLMEIEKLLESGTDPAEIAVIYYRHAQADQLIRLMGRKQLPYALRKQLN
                     SLDVPLVRQILSVMEYAGKEHRQPNSAEHLLFEMLYFPFFGIHRHDILSLAAYMQGKR
                     TNNNWRQVIADPDLMAQVKLRDPEALQKLESRLTLWISEAGSLTLPMLLEKIFEEGGV
                     LAYIIAHHDRFFLLEAMHAFFDYVQAESAADPEMRVEDLLEQLNLMRRYHITLPVWRT
                     FGNRNGVQFVTCHSAKGLEFDHVFLIGCNSNQWESSRRNSNNFSLPDTLTFSGGSNEL
                     EAQRRLFYVALTRARKGLQVSYAETDNNAKTLEPSRFVEEMAVQDLTIRRSGSLDDEK
                     LAAIELLSLGTDKTPTAELFDPDWLTRKTEHLSLSASMLNAFLKCPVRFYFQYLLRIP
                     QARNDSMEFGNAIHLALQRYFNEVRETGNFPPPEKLVQPFRLYMQQHREAFTDKQFRN
                     RMELGEMILPEYYAQHVSSWNKVVVTEYPVRDVTWKNIPINGKLDKIEFNGNEVHVVD
                     YKTGSYRLAKQKMKGPTDKDPLGGEYWRQMVFYQILMDQQRQKPWRMVSGEFDFLEKN
                     PDSGKYEKERLVITSDDTAIVGRQIEEVYGKIRSLDFTEGCNEPDCEYCNLLKYE"
     gene            27054..28856
                     /locus_tag="PK742_00565"
     CDS             27054..28856
                     /locus_tag="PK742_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQU75108.1"
                     /translation="MAKKSLYEPLLAEVAWEVCNQIGGIYTVVRSKAPYISEKWGEQY
                     CLIGPLLDDRLPAEFEEATDPDPNDPYTKAVDKLRADGVEVFFGRWLTQGTPKVVLMR
                     PEQANDQMKDIRQYLLKHHHINIFNPDDLLQQVITFGHLVYSFFAALEEESGGRDIIG
                     HFHEWMTGMAIGEIAREKRKIKTVFTTHATLLGRYVAMNDTNFYRNLPSIEWLPAAQK
                     FYIEPQVRLERLAANDCTVFTTVSDVTGKECEFLLGRKPDAILPNGINLYRYMATHEL
                     QNLHGQFKEKIHEFTMSHFFHSYTFDLDNTLYFFTSGRYEFHNKGYDLTLEALRLLNE
                     RLRKEKTDITVVMFFITKQPFESINPKVLQSRAVMDEIQNTCEEILKQIREKLFYAAA
                     ANADHHLPALNDFVDDYWKYRYRKMIQSWKSDYWPIVVTHNLVNDAADEIIQSLRKTD
                     MINKPDDKVKVIYHPDFITPTNPLFGMDYPQFVRGCHLGIFPSYYEPWGYTPLECVAR
                     GVPAVTSDLSGFGDYCHRRFRNLEDKGIYIVKRRNKTFEESAQDLAEIMYSFIQLNRR
                     DRIILRNTVESNAENFSWENLVEHYEKAYRQVLA"
     gene            complement(28900..30768)
                     /locus_tag="PK742_00570"
     CDS             complement(28900..30768)
                     /locus_tag="PK742_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU75109.1"
                     /translation="MKKQLLFTALFISSYVVLQAQITAYPDTTICSGETVTLFSDASV
                     YCGDCYTYEELEYAPEETAGEDVTMVDDTYVGPYDIGFEFCFFGELYSEFYICSNGWI
                     SFIEPGAGWASNWTPDGPIPDAASNVPKAAIFGPWTDWHTGLCTGCVSYETVGVAPNR
                     RLIVQWEDVPLFSCTSDEGTFQIVLHETTNFIDNHLVNVLVCPTWDLGISTQGLQNED
                     GTIAFAVEDRNATDWEATEETWRWYTSYISWYDDEGTLVGNGPSIDVAPEETTVYTVV
                     QTLCDGTEYSDEVTVTVAENFSGTLDITDVSCGGLTDGAATITLDGAGPYTYDWSTGF
                     SGSNSISGLSAGSYSVTVTAPDGCQKTFDFDIIELEPLELVFDDVENVQCFGYSNGSA
                     TIEVSGGAVPYDITVNGESTGSFLTELSAGDYLVEVTDDNGCTTSSTLTITEPDEIFI
                     TGSADVAITYGLSTTLTAETNTLDLTGVTWAPDGGVLGCEDDPCFIYTVTPVVTTEYY
                     VSITDENGCFAIDTILVTVIYSNEVLVPNAFTPNGDNVNDLFQGIAYNLNSYHMVIYN
                     RWGQLVYETNTADYYAGWDGKYQGEEQEMGTYVYFVEALFNTGVSYSGQGSFQLIR"
     gene            30959..31126
                     /locus_tag="PK742_00575"
     CDS             30959..31126
                     /locus_tag="PK742_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75110.1"
                     /translation="MILQKVSFDLTLFAKEFLKAAGKLPEEEMSELRIWCLQVFGLHY
                     CREAVPEFDRG"
     gene            complement(31277..32887)
                     /locus_tag="PK742_00580"
     CDS             complement(31277..32887)
                     /locus_tag="PK742_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HQU75111.1"
                     /translation="MKRLLIPLMGLLWGCANVVAPTGGDKDNTPPVLLEASPAMESVQ
                     MKERTIILKFDEYVQLKDAFNQVLISPPLEEFPDIKIKGKNIVIRLPDSLLDNTTYTI
                     QFGESIADITEGNPYSQFVYAFSTGEVIDSLYINGTVTDIWTGKPAANSLVLLYRNTE
                     DSAFTTQKPTYFAKTNERGNFRISYVREGRYNLYALQDQNFNYFYDLPNESIAFLDSS
                     LLVSQPVVQADLNLFTEARQKPFVSDARAVEPGLVRIIFNQAPDSLRYQTEQDHGGIF
                     IQSGSDTLYHWSQVADTDSQRIRISAIEMDTFLVIPQKKLPDNFFDNKLETAVPEPSG
                     GSRNRFTAQELHQPYQLLMQRPIAAIDSSRMILKADSLDEKIEWSVQTDTNDLRRLII
                     STQWKADSIYTLTLLPGALTDLYGIENDTITRSFRIRSPKEYGRLEVNVTDSINRPII
                     LQWLDKSGEVLAEKIPEWNDHNYLWQLQDLLPGTYIIRAIIDENGDGKWTTGSLAEKR
                     QPEPLLYYQAEVQIRPDWEYTLNFKLSL"
     gene            complement(32884..>34220)
                     /gene="mnmG"
                     /locus_tag="PK742_00585"
     CDS             complement(32884..>34220)
                     /gene="mnmG"
                     /locus_tag="PK742_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQU75112.1"
                     /translation="PASGITEQLVELGFESGRMKTGTPPRVDGRSLDYSKMEEQPGDV
                     NPGKFSYTDTKPLQLQRSCHVTYTSQEVHDALRKGFDESPMFSGRIKGPGPRYCPSIE
                     DKIDRFASKDRHQIFVEPEGWNTVEVYVNGFSTSLPEQVQFEALRKIAGFENVRMFRP
                     GYAIEYDYFPPVQLTHSLETRLVRNLFFAGQINGTTGYEEAACQGLMAGINAHQSVHD
                     LPPFEISRSEGYIGVLIDDLINKGTEEPYRMFTSRAEFRVLLRQDNADLRLTEKSHTL
                     GLADIGRLHQVIEKRAQTSAITDLLQKLSVAPDQVNGLLEELGSAPLKQKMKATSIIS
                     RPHVGIKDLATVIPDLKTALAAFSEEYLEQAEIEIKYEGYVAKEQEIAEKMQRLEDVS
                     LSESLDYHKLQSLSSEAREKLSRVRPRTLGQASRISGVSPADISVLMVYMGR"
BASE COUNT         8716 a         8038 c         8305 g         9161 t
ORIGIN      
        1 gataagatga aaaacagacc accattcacc tttgcattca gttcagtaat ttcacccaac
       61 agacaaaccg gcagatgaaa aagatattca ttacacttct tatatttatt ttttattccg
      121 ttgcagatgc acaaatgcag atctatccac ctcattggtg gacaggtatg caggacagca
      181 cggtgcagct gatggttcat cgcgaaaatg tggctgcaac caccccaact gttgaccatc
      241 cgggtgtgcg tctggactct gtgatccgca cagaaaatcc aaactacctg tttctgaacc
      301 tgaccatctc cgaaaatgca cggccaggaa taatgaccct tcgttttgcc ctccggggca
      361 gaccggaagt ggcctactat gagctgaaac aaagagccga aagaagtctt gcatacggac
      421 tggatggcaa tgacttcatc tacctgatca tgcccgatcg ttttgtgaat ggtgatcctt
      481 ccaatgatat cgtgccgggt acacaggaaa caaccatcga caggagtgtg ataaacgctc
      541 gtcatggagg cgatctgcag ggcatcatcg gcaaactgga ttacctgcag gatctgggtg
      601 ttaccgccac ctggctgacc ccacctcagg aaaatgatca gcctaaatac tcttaccatg
      661 gttatgccat gaccgatcat tatgacatcg atccccgcat cggcgatctg tctctgtatc
      721 gtcagtatgt gcaggaaagt catgccagag gaatgcaggt ggtgttcgat gtggtgcata
      781 accatatagg caatgaacac tggctcttta cagacgcacc ttcggccaac tggtataacc
      841 aatgggacag cttcacccgt accaactacc gcacatcctc cctgatggat atgtatgctt
      901 cggaagaaga cagaaggctg atgaccgatg gttggttcga cacccatatg cctgacctga
      961 accagcgcaa ccccctcgtt gcgaagtatc tgacccaatg gaccatctgg tggatcgaag
     1021 agatgcaggt ggatgccatc agactggata cctattccta tgcagatcag acctttctga
     1081 gccaatgggc cctggatgta ctgcaggaat atcccggctt caccctattc gctgaagtct
     1141 gggtgaatgg tctgggagtg cagggatatt acaacggtaa caataacctg aaccgcaatg
     1201 tcaataccca cctgagtggt gtgctggatt ttgaaatgaa ctggaccctg ggcaaactgg
     1261 tcaatgaaga tcccggctgg aactcgggca acggcgcatt atacctgtat ctgtgtcagg
     1321 attatctgtt cggcgaaaac ggtggagcag ataatgtcat ctttctggat aaccatgaca
     1381 tgagccggtt ctatgaagtg accggaaaag atctggataa aatgaaaatg gccaccggca
     1441 ttctgctcac cacaagaggg atacctcagt ggttctatgg aagtgagata ctgatggatg
     1501 aaccggcaga ccctatggac cgggtacggg aagattttcc gggtggttgg ccgggtgata
     1561 ctctgaataa gttcactgct gcagacagaa gcccggaaga gaacacgttc tttcaatacc
     1621 tgcgtaccct tgcacgctgg catgcacagg aacccgcttt gaagaatgga aaactcatgc
     1681 agttcgtacc agataatggt atatatgtat atttcaggtt tacggatgat gcatccgtca
     1741 tggtgatcgt caatggaaac gctgaagaac aagagctgga taccgctcgt ttccgtgagc
     1801 ggcttcatgg atatacctct gccgtcaaca ttgagtccgg tgccctactc aaatctctgg
     1861 aggtgttgca actgcctgct atgagtacca ccattcttaa actgcatcca tgaccggttt
     1921 cgaacaactg gacgcacata ttgcgagatt catcaacctg gatgatgcgc agagacaata
     1981 cattcatgga ttgctggaac aacgggaggt tcggaaaaaa gaccatcttt ttcgtgccgg
     2041 tcagatatgc agatatgaat actatgtggt gcggggttgc ctgcgagtct attttctgga
     2101 cgatcatggg tctgaggtca acctcatgtt tccggtagaa gactggtggc tgggagacct
     2161 catcagtttt acagaacaga caccgagtgc cctttatgtg gaagcgctgg aagacagtca
     2221 gctgctcatg atcaataagg agaataaaga atcattgtat gaaaagatac ctgctttcga
     2281 aaggatgttt cgggtgctga tcctgcgcaa cctggctgtg ctgcaacacc ggctcattgc
     2341 aaccatctca cattctgcag aagaaagata cctcgctttt attgagaggt atcctgacat
     2401 accacagcgt attccacaac atcacattgc atcctacctg ggcgtttcac ctgagtttct
     2461 gagcaagatc agaaaaaaac tgtcggcaca gtgagggcat ttactgctac ctttaactat
     2521 ggccctacgc aaacgcaata agtctgccaa gaatgtcaag aaaaagaccg gtcagtctcc
     2581 gggttctctg atctataccg gagtgcagca tctggacaag gtgcacatag aatacattga
     2641 atacaacgag gtcgatcttg tgcagcgtga ccatgtaagc ctggaagatc ttgaggcgtg
     2701 caggaactct gacaagatat cctggatcaa cataacagga cttcacgata cagaactgat
     2761 aggcaaggtt ggagagatct tcggcctcca caccttgttg ctggaagaca tcctgaacgt
     2821 caaccaccgg ccgggttatc atgaagatga acaggtggtt ggtgcatttg tcaaaatgct
     2881 tacccctgac cccggcgatc ctttctcgca aacagaacaa gtggccattc tggcgggcag
     2941 cggttttgtg ctcaccttcc aggaatatcc gggcgatgtt tttggatctg tcagggaacg
     3001 attactgagg ccaaccacac gcattcgcag cagaaaggca gactacctgg cctatgccct
     3061 gctggatgcc gttgttgacc agtatctggt ggtgatagat gaactggggg tacgggtgga
     3121 agatctggaa gacagcatga tgcaggtgtt cagaaaatca ctgctggatg acatttatat
     3181 gtataaaagt gatatcaatt tcctgcgaaa gatcatccga ccggtgaggg agatcgctct
     3241 ccagctggaa aaatctgagg ctgaatttgt agaagatgcc accggaccat ttctgaaaga
     3301 cctcgtagat catgcagaat atgcagcaga atctgtcgag acctttcgtg aaaccatcaa
     3361 tgacctgctc aatttctatc acacacagag tgccaaccgg ctgaatgaca tactcaagat
     3421 cctgaccatc ttttctgtgg tattcattcc gctgtctttc ctgaccggtc tgtatggtac
     3481 caactttgaa ttcattccgg agatccacag agaacatgcc tggcctgttt tcctgggcgc
     3541 agaagctgtc attgccattg tgatgatcct cttcttcaag agccggaaat ggctgtgatc
     3601 aatgtccgcg aatgcgctgc accaacatcg ggtttgccac cggatccagc cagccgaaca
     3661 attgctgcat atcttctgca gacatggctg tgcaacctgc tgtaggatac ccctgatcct
     3721 tccatatatg cataaagata caggagccat aaccaggcat gacaggatct gtgttgtatc
     3781 ctacccagat gcctttcgaa taaaggtcat cgctccgtcg catgtcttcg gcactcaggt
     3841 agctggcaac tgttctcttt gacggcaggt tgtatgcccc agacaggctg tcatccacgc
     3901 aaatatcttc atcggtcatc tgcctgtacg gcatgcgtgt atcagcatcc ggtgcatatc
     3961 caaaagcaaa cagcagagca tagctgccgg ccggcgaaca cttatcacct tcctgtttcg
     4021 tcctggaata tcccattggc aattgcaccg gatcggcagc aagacctttc ctgcctacca
     4081 tgacagtcat gctgtccaat atcttccatt gccggcccgt tcgctccagg cagaacaggg
     4141 ttgccaggct gtcaagttcg gtctctgttt ccaccagcaa aacctgcgaa cagtgagccg
     4201 gaaccggcag ttccgggtct tccgaaaggg tacaggccag aaagagacag aaaataaaga
     4261 ctccgacagt atgcttcata tcctaaaata cctgaatttt atgcttcatt caaacagcat
     4321 gaaacgcatt tacatccttc tttccgcttt cttccttttt tctgcagccc acgcacagat
     4381 aactattact gttgatgacc tggcaacaga gggagacgtg atctatttca gcaacgccac
     4441 ccctttggga gaatttgatc tgaccgccga tgggcctgat caggtatggg attacagctg
     4501 gctggatcag accggtcagg acagttctgt atgggtagat ccgtttgaga ccaatccggc
     4561 atacttttta ctgtggtttg gttcatctgt agccgaagaa accggacaga ctttttcttc
     4621 tgattttttc agcctggaag atatctacaa cttttacgat cgcaacgaag gcgaattgtc
     4681 tattaccggt atagctggta ccgtgaccgg cattccatta cctgcttcat ttgatgatgc
     4741 agatgtttta tatgaattgc ctcttaacta taatgattct tacagctctg cttccggttt
     4801 cgctttttct cttcctggca tcggatcctg gtctgaatcc cgcgacaggt cttgtctcgt
     4861 tgacggatat ggcgaagtga ccacacctta tggaactttt gatgcgctgc gtgtgaactg
     4921 cgaactgctg gttacagatg tggtggaata cagcggtata gagtttccta tttcctatac
     4981 cacccgcgaa taccgctgga tggctcccgg catgaaaatt cctgtgctgt tcatcagcag
     5041 tcaggagatc ctcggatttg aaaccgttac caatattcaa tacatcgatt ctctggatgg
     5101 cgctcctgtt ggtattcagt tgccaacggc tgttccggaa attttggtga ctcccaccct
     5161 cgtgcagcag ggtttgtgtc agttgcaact cggagatcct gttggtgcag atgtcatcat
     5221 tactgacctg caaggcaaac aggtattgga aaatattcat gtagcacccg gacagtctgt
     5281 cagtgcctta ccggtgggtg gcttacatgc aggctggtac tggtgtatgg taaaacaagg
     5341 tgaacacctt gtttccaggc cgtttctgat agcagaataa aaaaccggag gcagattgac
     5401 cgcctccggc acctatgaaa acaaccacta cttagaagtc ttttttgttt cggtctgtct
     5461 gacctgttgt atgtataaaa accctgaccg ggcaaaaagg ttcaggcatt tttttgcttt
     5521 ttaacgatct tcagaacaaa tgacagttct gtgaagtttc ctacatttga aaagcctcca
     5581 ataagatttc catgagctct attctgcttg gttcagcatt actggtactt gtttttgttt
     5641 ccgttgtctg gatcctatca gtcctggcaa aagacatgag cattgtagat attgcctggg
     5701 gcctcggttt tgcagccctg gtgatctttc agtgggtcag atgggcaccc gcatcgccac
     5761 gcagttttct ggttggctac ctgattctga tctgggggct ccggctggct gtattcctgg
     5821 ccatcagaaa cctgagaaag ggagaggatc caagatatgc cgcctggcgc catgactggg
     5881 gaaagaatac gtggtggatc tcttatttca aagtattcct gctgcaaggt gcactcatgc
     5941 tgatcattag tatgcctttg catgtaacta ccgctgcagg caaaagcatg ggaagctgga
     6001 cagactttgc cggcaccctg ttgtggctgg gcggattctt gtttgaatcc atcgctgact
     6061 ggcagttgta ccgcttcaaa aaggatcctg ccaataaagg aatggtcatg gacaaaggtt
     6121 tatggaaata cagccggcat ccgaattatt ttggagagtc actcatctgg tggggaatat
     6181 tcctggtagc ttttggtgcc ggcaccgctt atcttagtgt gataggtccg gtacttatta
     6241 cttttctgtt gctgcgtgta tccggggtca ccttgctgga acagcgctac gagggcaatg
     6301 acaaatatgc agattacaaa cgaagaacca gtgccttcat tccgaggcca ccaaaacgtc
     6361 aggtatgaaa ataggcctgt tcttcggttc atttaatccc attcatattg ggcatctgat
     6421 cattgcccag cattttgcag attggacaga tctggaccag gtctggctga tcgtgtcacc
     6481 acagaatcct tttaaggaaa agtccggatt actggatgag aagcatcgcc tgtatatggc
     6541 caatattgcg gtagaagaca atgacaggat cagggcatcc aatgtggagt ttcatcttcc
     6601 ccaacccagc tataccattg atacactgac ctatctggaa gaaaaatatc cggatcatcg
     6661 ttttgtactg ctgatgggta gcgataatct tcccactctg cacaagtgga aaaatgccgg
     6721 tctgttgctg cagcggtatc ccattcgggt ctatctgcgg ccgggacaga gcaatacgaa
     6781 tccctatccg gaggctgata ttacttttac agaagcgccg ctgctcaata tcagtgccac
     6841 cttcatcaga caggccgttc gtgaaggccg tgacatccgg tatctggtac ctgaaaaggt
     6901 gtatgagtac atacgatcca tgcatttcta tcaatgagca caattgtgga ttactgcggc
     6961 aggatggtgg acagcttggc gaagaacatg tagatatgac tcaggaaatc cacacaacta
     7021 aaccataccc tttaaaaaac ggttacttat gtggatgaac acccggaaat acacagtgat
     7081 tccacgcctg ggtatgtatg tcgggagaaa agttatgctg ttgaaccgtt atccaccgga
     7141 tgtgtatagc tcagaaaaat gcagcaaata cgccatcttt acctgtgcac tagaagtgga
     7201 ttacctgttg taaaaggagg atattgcttt ttccaaatac ttaccgacaa aacaatcaac
     7261 gaatccacag tactgatgat gatgttttct tttacttttt aaaatcatag aaattaagga
     7321 caccatggaa attgtggaaa ccggttggga gacaaaagga tatctggata ttccggtaga
     7381 tcctaccctg gatctggtgc gtgaaattga caagctgaaa cgggaaaaga atgcagtgct
     7441 attggcacac tattatcagg acagtgatat acaggatgtg gccgattata taggcgacag
     7501 tcttggtctg gctcagcagg ctgcccgcac taaagctgat atcattgtgt ttgcaggggt
     7561 acatttcatg gccgaaacag ccaagatcct gaatccgcag aaaaaagtac tcatgcctga
     7621 tgcacatgca ggttgcagcc tggcagattc ttgcccgcca ccacttttca ggaagtttaa
     7681 agagcagtat cctgatcata ctgtgatctc ttatatcaac tgcactgcag gtatcaaggc
     7741 attgagtgat ataatttgta ccagcagtaa tgctgtgaag atcgtagaga ccctgccccc
     7801 tgatacgccc gtcatatttg ctcccgaccg caatctggga aggtatgtaa tggaaaagac
     7861 aggcagagat atggtgctgt ggaatggcac ctgtatggta catgagatat tcagccagga
     7921 aaagatcaca aaactgagac accggcatcc ggatgcaaag ctcattgcgc atccggaatg
     7981 tgaagaagcc atcctggaaa tggcagattt cataggatcc accaccgggt tattgaagtt
     8041 tacccgggaa gacaatagcc cggtttatat tgtagccaca gaaacaggta ttctgcacca
     8101 gatgcagaaa gcatcaccac acaagcaatt cattccggca ccgccagata acttatgcgc
     8161 atgtaatgat tgcccgcaca tgaagagaaa taccctcgaa aaggtatata cctgcatgcg
     8221 ttatgagcag cctgagattc tgatggacaa aacaatactg gaacaggccc ggaagcctat
     8281 tcaaaggatg ctggatatct ctgccagcct gggactgtag atagtattat acgtcgtact
     8341 tctccatcat actcagcacc ttagcgatct tgtcataatt cagcgaatag aagatgaact
     8401 taccttcccg cttggttttg accagatcag cgttacgcaa aatgcgcaga tgctgtgagg
     8461 tgatggattg ctcaagaccc agggtattgt agatcttgtt tacatggacg ttcttattct
     8521 tgtcaataaa gcccatgatc ttcatgcgca gcggatgtgc cagggcacgc atgatctcag
     8581 cagacttttc cagtttggat atatcgacac tcagaaatgc tttctgatcg gtagtctttg
     8641 ccataataga atttgattgt gatgggtgtt aggaaatgtc gcgaatgaag ttgccaatgc
     8701 tcttcaacct gtcgttgtcc agctgataga aaatgttctt accacttctg gtggtagtaa
     8761 caactcctgc cctgcgcata atagccaggt gctgggatgc gaccgactgt tccagacgaa
     8821 gcttgacgta gatctccgtc acactcatct tttctttgtc agttaacatt ttgatgatct
     8881 tcaaacgcag tttgtgattt actgcacgaa gaatcagaac ggtcttccgg aggtttttgt
     8941 actcaagctt gatgttaccc ttcggagcgt tcgcaagttt aatacttggt gtagatgcca
     9001 taatcagaac ttttgtgaat caattaacac gtggcattca gagggtagtc caaaaataga
     9061 atatatcaga aatattacaa aattaatacc tgtttacctg caggttattt tccgtttgta
     9121 tggtaaaact ccttcagata tacgggttcc agataggccg gatcctcatg aataccttta
     9181 ttatatgcat ccagggcaat tttgttcatg ccggacgata aaatgataaa atttgttaat
     9241 attttggagt tcttgtcttc caggaggtgt actgccttat ttgaaccgct gccaaaaaaa
     9301 caggtttgtc tttgttctgt ttgttctctg aagggtttgt tatccagtat gtatgcttcc
     9361 ggttcacata gcagagtgcc tgtatgatca aacagggcgg tgtacacttc catccgcctg
     9421 gcgtctatca tggggcagta cagggtttcc tgcataggat attgatttat ggcgcaggca
     9481 gtcatacaag ccagcgttga aacaggaatc aacggtatgg tactgccata gcagatacct
     9541 tttgcaagac taagtccgat gcgtaatccg gtgtaagatc caggcccgat agatatggct
     9601 actgcatcca gcgattggat agaaaacccc gcccggtcta acaggctctg taccataggg
     9661 ccaagctgcg aagcatggtt ttgtgcttcc tcacccctga tctccagtag ggtgtttccg
     9721 tcggctgcca gagaaacact ggaaagcggg tgactgcttt ctatctgcag aatagtggcc
     9781 actcagaact gaataggaaa ggtcatttct tcttctgccc gtatcacggt agcagtggtt
     9841 gcatctcctt tttcaaacat acttaatgcc ttcatgtaag acatcatatc tgcaacgttg
     9901 taatcgccca gtctgataac tatatcgcct gtttgcaggc cggcatttgc agcagttttt
     9961 ccatctgata caccgtctat gcgcatgcct ttgccttcga aagtatagtc aggaataaca
    10021 ccaagaccaa ctgtgaatct gggtgtttct tcgctttctt cttctttggt tctggtaaat
    10081 tcaacctgat cttcatcgtc cagatctgcc atcagggccc ttaccagatc atgcaattcc
    10141 acaatgcctt tatagttgat cttgtctgca tcatctgaag gtttgtgata atcttcatga
    10201 gtgccggtaa aaaaatgcag gacaggaatg tccttcaggt agaacgaggt atggtcagat
    10261 ggtccgatgc cgctgctgta ggtcttaatt gtaaagccct gcatcttgcc aaggtcatct
    10321 attctggtcc aggccggtga ggtgccgtag ccgttcacta tcagggtctt tatactgtcc
    10381 agtcttccta ccatatcaaa attcagcata taattatagc agtcgtcttg agcggtcaga
    10441 aaatcagcga tactcttgga accgtacaga cctaattctt cgccgctgaa ggcaatgaac
    10501 agatagttgt agtgatcgtc ggggtctttg gccaggtttt cagccagctg caggatgaga
    10561 gccacaccac ttgcattatc atcagcgccg ttgtggatgg caggctgacc acggtacagg
    10621 gatccgcccg attcaccata gcctagatga tcataatgag ctcctaatat gatggtacag
    10681 ggcgctccat tgtcaattct ggcagctatg ttctttcctt cttttctgac cggatgtata
    10741 tctgctccaa ccaggatctc caaaccctgg gtcagagtgt tggcggtttc aggttctata
    10801 aaaagaacag gaatacctgc tgcttctgct ctgctgctgt aatccagtag caatgaaggg
    10861 tcataatcct ggtccatatt ataaaacaag acggccacag cgccatgttc catggccagc
    10921 tctaccttac ttcgctcatc ggcataagga taaaaatcgg tatgcgggtg ggtgccttcc
    10981 ggtgtgctca gttgtataag caggatcttt ccctttgctt ccaggccctg gtaatcatcg
    11041 tgttgcagtt caggtgctgt gatgccataa ccggcatacg ctacctgtgc ctgaacctgg
    11101 ccactgccgg aaaagaagac cggctgtgca tcggctcttt tgctattggc taccacaaaa
    11161 ttctgttcgc cggcttcttt accggtgctg aaagaaaaca cctgaaagaa cccgctgttt
    11221 tcgccggccg gttgcaaacc gagtgcagag aatttaccgg ctatgtagtt ggctgcgatc
    11281 tcttctccac ggctgccggt ggcacgccct tccagacggt catcagaaag aaattcaaca
    11341 gtttctttga tctgatcggc agcattctgc ccttgtacga agccggtcag gaacagaaga
    11401 ccggaaagaa tgaaacttct catactgtaa agttcaccat tcagagcgaa atggcctatt
    11461 tgctgagtag tttggtgtaa gcttccggat ttttcagcgt tctgatggct tcctgaatgt
    11521 cagggtcata gcggaaagac acttccaccc ttccattgta attataatat cttcctgcta
    11581 tctctctgtt gagttcgaac aggatctcat ccttgaattt atacagatca ttctgcttgt
    11641 catgtgtcac cttggcatgc aggccgtcta tctcattttt gacggcatca taataggcat
    11701 cttcccgggc actctcttca aaggcagcca ggagttcttc tgtttcagtt gtgtattcat
    11761 aggttctgcc cgacatgaag cgcacaaacc catcgtattc ttcgtctgta agactaaagc
    11821 tgttggctgg actgatgttc gggtggctgt agaaatactg cgtggcgtaa tggaagatgt
    11881 gattgccctg caggaggctg atggtgatat cgcggtaatt ctcttcacct acagctacat
    11941 cagggtcaat tcccccgcca tcatatacag ttcttcctgc ccgggtggaa aaggccacct
    12001 tcagagagtc cgggatcttt cctacagaac cgtcttcatt tctgttggcg taatcgatgg
    12061 cctgaataca gcggccactg ggtatgtagt atttggctgt ggtcagttta agttttgtct
    12121 tataagatac atctcttgtg gtctgaacca gtcctttgcc gaaggttttt tgacccagaa
    12181 tgacaccacg atcatagtct tgtatcacac ctgcaacgat ctctgaagca gatgcagaac
    12241 ttctgctggt aagcaccacc agcggtatct cggtatctac cgggttgttg atggaaacaa
    12301 aagatttgtc ccattcttcc acacgaccac gggtactgac cacttccagt ccttttggaa
    12361 taaatacatt ggaaacattc acggcttccc gcagcaaacc tcctggattt ccccgcaggt
    12421 ccaggatgac accagacaga tcaggatgat cttcctgaag tttcttcagc gcatcagcta
    12481 cattccttcc tgcatcttcc gtgaactggg tcagcatgat ataacccgta tggtcatcca
    12541 ccatcccgaa ataaggcaca ttgccctggt tgatctcttc acgggtaagt tccagttgta
    12601 atgtttctcc ggtgcctgcc ctgaaaacag taagtgcgac cggggtaccg ggagatcctc
    12661 gcaacaattc gctgatggat gactcatcat tttccagaac ggtgtgatca tcgatctttt
    12721 ttaattcatc gccgggctga aggcctgctt tgaaggcggg aaaaccttca aacacatcaa
    12781 ccacggtaac cttgccgtca accggtctga tggtagcacc gatgcctccg tagttaccgg
    12841 ttatctggaa tttatatcct tccagttctg cttctgatat atagttggtg tatgggtcaa
    12901 gcgattccag catggcatca atgcctgtgc gcatgatcct gttcgggtcc agatcatcta
    12961 catagtaagt gttcagttcc ttatagacag aggtgaaaat gtctatgttc ttggatatct
    13021 cgaacagatc gcttcccgga gcgttgctgt tgaagaggag ggtaagtgcc agaccggagc
    13081 ttagtacacc ggcaatagcc aaagtttttt tcatatactt aagattcacg gaaccgggtc
    13141 atggccgtga ccaccccacg ggtggcatct gctcagtctt ttcaatccga gccagccgcc
    13201 cttgagcgga ccgtatttac gcagggcctc gatcatatac tgcgaacagg taggggaata
    13261 ccggcagctg ttggggaaca aaggcgacag gctgacctga taaaaccgaa ccaatagcac
    13321 cagcagaccg ctcacccact tagagaccac gttccagttc ctttgataaa cgttctaaag
    13381 ctaaatggaa ttttcctttc agctcgttaa atgttgttgg ctttttactt tgatacagac
    13441 agatgatgtc tgcatgaata ccttccggaa tctgcagctg atgttgttcc aggcgaaagc
    13501 cctcccgcaa gagccgcttg atccggtttc ggtcggtggc tttgggatga tttctcttag
    13561 atacagaaaa ggcgatccgg taagccacat cgcccttagc gggttgttgt cggtacacca
    13621 ggcgcagggg ttcctgaacc aggcttttgc cttcagtaaa cagcaaagaa atggcagccc
    13681 tgcttttcag gcgatgagta agaggaagat cgtatgcagg atcaggcaag tgggcctccc
    13741 gcttcggtta cacgcggaaa cgcatcatgg tacgctcgtc agaaacggtc agtttcttac
    13801 gtcctttccg gcggcgacgg gcgatgacag cccggccatc cttggtcttc atgcgactgc
    13861 ggaatccgtg cttgttcact cttttgcgct tggatggctg aaaagtaggc ttcatctgtt
    13921 taaaaatttg ggactgcaaa gataagcgct ggcattgaaa tgcacaacat atcaacagcc
    13981 ggtatcaaaa tattgatcag gagttcatgg aaatcaggaa ttcttcgttg ctcttggtgc
    14041 ctttcatgtt ctggaggagg aaattcatgg attcttccgt gttcatgtct gccaggtgat
    14101 tacgcaggat ccacatgcgc tggagcacat cacggtcatg cagcagatca tcgcggcggg
    14161 tactggatgc cgtaacatca atggccgggt agatgcgctt gttggacagt ttccggtcca
    14221 gctggagttc catgttaccg gtacccttga attcttcaaa gatcacttca tccatcttgc
    14281 ttccggtatc gatcagggca gtggctataa tggtaagaga accaccattc tcgatattcc
    14341 tggccgcacc gaagaactgc ttgggtttgt gcagggcatt ggcttccaca ccaccggaaa
    14401 gcacttttcc ggaagcgggg gcaaccgtgt tatgtgccct ggcaagtcgg gtgatggagt
    14461 ctagcagaat gaccacatcc tgaccgcatt ccaccaggcg cttggctttt tgcaatacga
    14521 tgctggacac ttttacgtgc ctgtcggcag gttcatcgaa tgtcgaggcg atcacttctg
    14581 cattcacgct tctttccatg tctgtgacct cttccgggcg ctcatctacc agcagaatga
    14641 tcagatagac ctccgggtgg ttgtgggcaa tggcattggc cacactcttg agcagcatgg
    14701 tcttaccggt cttgggttgt gccacgatca taccacgttg tccctttccg atcggggtga
    14761 acaggtccat gacccgggtg ctgtagtcgg tcgggctgta aaccaggttc agtttttcat
    14821 tggggaagag cggtgtcagg tagtcgaatg gtaccctgtc tcgcacttct ttaggatcgc
    14881 ggccattgat ggtctccacc ttgagcaggg cgaaatactt ctcaccttct tttgggggcc
    14941 ggatggtacc cttcacccaa tctcccgtct tcaggccgaa taatttgatc tgagacggag
    15001 aaacatatac atcatcggga gatgacaggt agctgtaatc ggcactgcga agaaaaccat
    15061 aaccgtcggg catcatttcc agtacacctt cgcccggaat ggctccctcc aggtcgatat
    15121 tgaacctttc acggcgtttc tgcgggtatt gatgacgatt ctcctggcgg ttttcttcca
    15181 ccggtcttac cgtatctgtt tctcttaccg cttcttcttt tcggggtgct tcctgcttta
    15241 ccggagtttc ctctgcaacc accggaattt cggccttctc aggctcttcc gccccatctt
    15301 cctttttctt acgcggacgc atattgcgct tcttatcctt tttttcagcc ttttgggtcg
    15361 tctcttcctg tccgttttct gctatttcct gcggagcaac agcctgggca tccagtatct
    15421 tgtaaataag gtcctgctta ttgagctttc gaatgtcttt gacctgcagt ttttcagcga
    15481 tctctttcag ctcaggaacg agcatttcat tcaattgaag aatatcgtac atatgaaatg
    15541 gaaatacggt tacaacgtaa aggttggatc agtaaatggt actaaaggga aataaggttg
    15601 agtaagagag ctgtgctcca cgggggcggg aacacttcgc aaaaataatg ttcctttttg
    15661 gaaaataaaa atgcccggcg gcattgggct gtccgttcta tctttgaaac aaaaatccac
    15721 ctatgctgga aatatccttc ctgcgaaaca atctctcgga agcaaaaaag cggttggcca
    15781 aacgcccggg ttatgatacc ggtgtgcttg atgaattatt ggcggcagat gatgaaagac
    15841 gcaggctgca ggcagatctg gaccaggtac tgggagaaca aaatcgcctt gccaaagaga
    15901 taggggcact ttttggccag ggaaaaagag aagaagctga acggctgaaa gaacaggttt
    15961 ccggcctgaa ggagcagagc ggcgacctgc agaaacaaca tgatgataca gccgatgcca
    16021 tacgcactat gctgctgatg ctgcccaacc tgccccagtc acgggtgccg gatggaagaa
    16081 gcgccgaaga taatgtggtg gtgaaggaag gaggcaacat gccggagctg acagacgatg
    16141 cacaacccca ctgggatctg gcagccaaat atcagatcat tgatttcgag ctgggaaaca
    16201 agatcaccgg ctccggattt cctgtgtaca ggggaaaagg cgcacgcatt cagcgggcac
    16261 tgatcgccta ttttctggac aaggcgcagg agcggggata cacagagtat atgccacccc
    16321 tgatggtgaa tgaagacagc gggtatggca ccgggcagct gccggacaaa gagggccaga
    16381 tgtaccacat gacggtcgat aatttctata tggtgcccac tgcagaagta ccgctcacaa
    16441 atatctaccg cgatgtcatc ctgaaagagg agcagctgcc tgtacgcatg accgcctata
    16501 caccctgttt cagaagagag gctggatctt acggtaaaga tgttcgtgga cttaaccgct
    16561 tacatcagtt cgataaggta gagatcgtac acattgaaca ccccgacaga tctgatgaga
    16621 ccctcgatga aatggtcagt tatgtggctt ctctcgtaga atcactggaa ctaccctatc
    16681 gcatcctgca tctttgcgcc ggtgataccg gatttgcctc ttcctgcacc tttgatttcg
    16741 aggtctatgc ggcggcacag aaaaagtggc tggaagtgag ctcggtctcc aacttcgaaa
    16801 gctttcaggc caacaggatg aagtgtcgct tcaaacaggc agaaagcgga aagaatcagc
    16861 tggtacacac acttaacggc agtgcacttg ccttgcccag gatcctggca gccattctgg
    16921 aaaacaacca gacacccgaa ggcatcagaa tacctgaagt gctcagacct tattgtggtt
    16981 ttgacatgat tgactaagca gatcagttcg atctggtcca ggattcggtt cggtgcagaa
    17041 aggctacgta tcctcgtatg cgtaaggtgt tcttgccgtc catccacatt tcgcacttat
    17101 aggtcttgcc attcttcgga tcatagatgg tgccgttctg ccagaaccca tcctcaaatg
    17161 tgaagttctt taaaatgacc agacccatca ggggcgtgct gcgtttggat gcatcagggt
    17221 tctcatcatc cacctttggt gtaccattat cttcgttggg ttctttcagc cagactagtt
    17281 taccgtagta cttatcaccc ttcttgtaga tctccacttt gccgtctcca tcagcactca
    17341 gccacttacc tacaatagca tcctggtact gggctttgga cgtgacagcc atcaataaga
    17401 aaaagcaagc tgtgaatgca ctgcgcataa catggatttg gatcaatatt aatgcagcag
    17461 gccgttttct gcaagccatt gggcgatctg tactgcattg gtggcagcac ctttgcgcag
    17521 attatctgat accacccaca ggttgagggt gttctctgca gtaaaatcac gacgcacccg
    17581 gccaacgaag acctcatctt ttccttctgc ctcaaggggc atcggatagg ccgcatgtgc
    17641 agggttatcc aacaccacga cacccggcat ggatgccatc agccgacgca cttcatccag
    17701 atcaaaatcc tgaccgaagg tcacattgac actttcactg tgtcctcctg aaaccggtac
    17761 cctgaccgct gtagcactta cctgtatgct gtcagatccc aggatctttc tggtttcatg
    17821 atgcagtttc atttcctctc tggtatagtc gttatccagg aaaacatcgc aatgaggaat
    17881 gacattcatg tctatccgat gcgggtaagc ggggttctct gtcagcctgc agttggagct
    17941 tctttctgta tgcagttgca tcagtccttt tgcacctgat ccggaaacag actgataggt
    18001 gctgaccact atacgtctga tgccataggc ttggtgcagt ggtgccagtg ccatgaccat
    18061 ttgaatagta gaacaattag gattggctat gatctgtcgc ttgccggaaa tggaatcagg
    18121 attgatctcc ggaaccacca gggcacagtc agtttccatt ctgaaggctg aagaattgtc
    18181 tatcacccag gcaccctgtt cttcgaatgc cggagcccag gccagtgagg tggtggcacc
    18241 ggcagagaac aatgccagat caggtttcag ggtcagggct tcttcaacag ttaccaccgg
    18301 tatcatgtca tcattaaatc gcacactttt tcctgccgaa cgttcagacg ccaccggtat
    18361 cagacgggtt accggaaaat tgcgttcttc cagtacccgg cgcatcatct gaccgaccac
    18421 tcctgtggcc cctactattg ctacctgcat aggttgcaaa ggtaagtccg tcagggttga
    18481 catgtatgca tataactata agttaataga gggctatgca tcaaccgtca cagagtcttt
    18541 gtaattttca gcgatatgcg caaagcagtt gttgggatat tactgatcct gctgacaggc
    18601 cgggtattta ctcaatctct gacagatgat ttcagcgacg gagaaatcac ttctgatcca
    18661 tcctggaccg gcgataccga ccagttcctg gtgaatgcat cacagcagct ccagcttcag
    18721 ggtgattgtg atgcaggtgg aatcaactac ctgacgctgg ctgccgccac atcagattca
    18781 gtagtgtggg agttttatac agaactcgac tttgacccga gctcttccaa tcacatgcgg
    18841 ttttatctgc aatctgatgc agcagatctg agtgccgccc tgcatgggta ttatatcaga
    18901 ataggtgaag atggtacaaa cgatgctgtt aagctgtatc gtcaggatgg taccggcaat
    18961 accttgttgc tgagcggaga aacaggagca gtagctgcag cacccgcagt cagggttcgt
    19021 gtgatccgca atgcagaagc cgtgtggcac atgctggtgg attatacagg cggaacatca
    19081 tttactgacg aaggctctgt tacagacaat acatataaca ccggttatta tacaggggta
    19141 tattgccagt acaccagtac acgttgtgat gcattttatt ttgatgatat atatatttca
    19201 ccactctatg aagatgatga accacccgtg ctcttatcgg cagcagctat ttctgatctg
    19261 caggtgaaag ccatttttaa tgaacctctc caggcttctg atgcagagaa tggttcaaac
    19321 tatactgttg atggcggggt gggtaaccct atagatgccg cactgcaagc gggtggatcg
    19381 tctgtggtac ttacctttgc agatgacctt cctgcagcag tgctgctgca cctggaagta
    19441 aatggcatag ctgatctggc cggcaatgtt tgtgggctgc agagccagcc tttcactaat
    19501 ttctcgccag gattatttga tctgcttatc acagagatca tggcagatcc agaacctgtc
    19561 caggagctgc ctgctgcaga atatatcgag atttggaatg ccactccctt tcctgtaaac
    19621 ctggaaggtt ggaccatccg cgatccgggt actaccagcc agccatttcc agctgttgtt
    19681 ctggaggccg gtgcttatct catcgtttgc agcgcaggaa atacggcaga gtttgaagag
    19741 tatggagatg tgttaggtgt tggctctttt cctgcactga ataataccgg cgattctctg
    19801 tatctctttg atgcagatga agaactgata cacagcgtct attatactga tagctggtat
    19861 gcagatccta tcaaggagaa cgggggttat tcgcttgaaa tgatagacac aggtaatcct
    19921 tgtcaggagg aagccaactg gatcgcatct attgagctga gcggaggaac acccggacag
    19981 gagaacagtg tggcggccga taatccggat gtcgtatccc cgtctttggt tagcgtattt
    20041 cccagttctg ctacttcatt atatatcaca tttgatgaat atattctaac cgaagcgctt
    20101 accactacca tgttcgccat ctcaccagac attggcagtc ctttgtctgt gtcagccgat
    20161 ccgtatgtgg ctaccgttat agaattacca ttgccggaag ccctggaacc aggtattacc
    20221 tacaccctga cctgtaccgg gcttacagac tgcagcggta atgcaattgg cgtacacaat
    20281 agcctggagt ttggtttgcc ggaaaccccc ggtcccggag atgtgatcat caacgaggtg
    20341 cttttcaatc cggctacagg cagcgatgac ttcgtagaga tctacaacaa cagcaatcat
    20401 tttatccagt tgaatcagtt gcggcttact gaacggaatg tggagaatgc tgccgaggtg
    20461 ctggagtatt gctatatggg tgaatatgga agattgctgg ctcctggtgc gtatttcttt
    20521 ataacagaaa aggttagctg gctggctccg ttttatgata tagcacccaa tgcacagttt
    20581 agtgaaaaca gtcagtgccc caatttccct gatgatgaag gaattgtaag cctggagtta
    20641 tcatcaaccg gtgtggtcct ggatcagctg cactattatg atgactggca ttaccccctt
    20701 ctggaggatg ataatggcgt gtcactggag agacttaggt tcgatgcgcc cacccaacag
    20761 gctgagaact ggcactcagc agcagaagct gcaggttatg caacgcccgg caggttgaac
    20821 tctgtggtca gctcctttga aggcaatgct ggaacctgga cactggaaag cgaagtcttt
    20881 agccctgatg gcgatggcta ccaggatctg atggtactta cttataccca accggcagag
    20941 ggtacttctg cttctttgtc tgtcttcaat cttgccggac aactggtagc tattcctgcc
    21001 agaaatcttt atttaggtgt ggaaggatta ctggtctggg atggtctgga tgacagtggt
    21061 aagcagctgc caaccggcat ctatatattg tacgtggaag aatttgatct gacgggcaga
    21121 acagatcatt ttaagtttcc ggtaaccctt gccagaaagc aataaaaaag gccggaataa
    21181 ttccggcctg ttataagctt aacggtacgt tactctatac ggagcaattc gtattcaatt
    21241 cttgtggtca catcgatctc ttctgtcagt gcaaccgttt cttcatatac aatgacctgt
    21301 ctgtctttct cgttcacttc gaacagaaag ttaaactctt cattgttgta acgtatctgc
    21361 agggcattgt catctgtgat atcccattcg aattcaatga agatgaattc atccggatct
    21421 ttcagatcga tccactggcc atctccattg ctgaagaagt acatatatcc ggaatcgcca
    21481 tactctgtta catcaggagg cagatcagtt gtagtgatgg tcaccttata gttgtctatg
    21541 ttccacctgc cgctctggcg ggtcaggtta tctgccaact gcctttgctt ggaacaggaa
    21601 ggcaacagca acaacagggt cagggtaaat accagtggag caaatattct tttcattacc
    21661 ttatctttta tttgttaccg atctcttctt cccgaacaat atggatctca gttgttacga
    21721 ccgtttccag gtcgcccaca aaaaaggatt cttcccgcac ccaccattgt tcatccttac
    21781 tcacttcatt tatgctgaac tctacttctt cagtaaaacc ttccagatcc atggttatgg
    21841 aattattggt gttttcccac tcaaaataag tggcaacgtt ttgcatggtc agggtatcat
    21901 actgtatcca gatgccggta cctgattcat agaacagaac ttcacctaca ttgaattcct
    21961 gaataatggt tggttccgta ggcgggtctg ttttggtaac actggtaacc ttgaactcat
    22021 caatgttcca gcgacctccg tcacgggtga ggtggttctg caatttgatt tccggagaac
    22081 aggcaacgaa cagcgtcacg agtgatgcaa acagaataaa caaggatctt ttcatagcgc
    22141 aggctttgtc agattgcaaa attaacgaaa tagttttcgc aaaagtacga gacccggagc
    22201 ctgagacagc ctgtttttca cgtaattgta aaaaggatca tgctggatgt ccattttagc
    22261 caacagggtc agcctgttta ccagtagcat atgggtcctg gttccgggta agaatacgtg
    22321 ccatgagtac tacatcgatg atggtatgtg caagggctgc ggcccatatg ccactttcct
    22381 tgtatagcca acccaggccg gcggcaatgg gaatcattac cacaccgtaa atgaatactg
    22441 catgatcccg ggggttcaga tatccgtgca gcgcaacgaa tatcagtgct gttggccaga
    22501 tgcccaacca tggttgtatg ccaccccgaa aaagtagctc ttcgccaaca ccagcactta
    22561 cggagatcat tatgatctcc aaccatccta cccggtaacg cagtaaaagt ttcctgaaga
    22621 actgtcctgt gtgttttaag aagcgcaatc tgagtaaccg caacagcaag aggaccgcca
    22681 ttataccata caatgcccca tacagcagtt gcaaaagcac aggttgtttc tcgccgtgaa
    22741 agaagttgct gaaggtttgt tcgtggaaaa cgatcatgag cagcaacccc agaccgccca
    22801 tgccgaagag cgtaaccgct gcggccagat agatcctgtg ccggccagac accctatttc
    22861 gttttcaggg tagagtttcc cttgatggat actttcatgg attctccctg catttccata
    22921 gacagattca ggttctgctc cagcttggat tcaatggacc agccactggc tacagccacc
    22981 gtgatctcgc ccttttggta gccactcatc tgtacctcat cgccggttcc gcccatcata
    23041 cgcaccattt cgcgatctac tttgaattct gactgcacac tgaccagggc aaacttgtca
    23101 tcaaagctgt tcagggtgaa agtattggtc acaatggcag gaactccgcc caggtccaca
    23161 gagtctgttg atttccaggt atcacctatg gcaataggac ggtgcggaaa ggctacaaag
    23221 ctgttgatga tatcctggtt catgtcactc atctgcagtg atgccatcag cgcttcctgg
    23281 ccaagctggg tattgatctt ggattgaatc tcttcaatgc ctcctatgtc cactacgtct
    23341 gtttcccggc tgatggtcag cttgaccggc atgtccacat aacaatccat gatctttctg
    23401 atcatatcaa tgtccggatc atctgatgaa actccattct ggaaagaagc ctccagagaa
    23461 tcctgcttga aatgtgcttt aaatgatttt acctgcgact gtaatacgat gttactgtcg
    23521 ggcttattct ccattacccg gatggaattg ctggtgctga tgtccatcag attgctgaag
    23581 gtctgaccga acatcgtttg ggtgatgttg gtaatcgtct gcatggaggt gttatagacc
    23641 ttacctgcgg gcgggttgaa ttgtatgatg acctgatcct taccggcttc gatcttctcg
    23701 ccggatgagt cgatcagaaa agtgataggc tctccatcgg ccagaccggt ggtgtcaata
    23761 attgagaggt cactggtggt gccggggttg ttgttcttgc actggctgaa cagtaaaaca
    23821 gaagccagca aaaggcttcc tgataagagt ttatgcatag ctttttggtg ccgcaaagtt
    23881 acctgaaatt tgttacattc gacgcccatg gaatcgagaa ccgccctcac cgaacaattt
    23941 aaaaaagcat acaacagtct taatgatgca cagcgcacag cggttgatgc cattgaaggt
    24001 ccggtcatgg tcattgccgg ccccggcaca ggaaaaacac aaatactggc ggtacgtata
    24061 ggaaatatat tacttcaaac agatgcgctt cctgagaata ttctctgcct cacctatact
    24121 gaagccggag ccatcgccat gcgtaaccgt ctgctttcct ttataggacc ggatgcctac
    24181 cgggtgcaga tccagacctt tcacgcattt tgcaatgaga tcatccagag ccatcctgac
    24241 ctcttcggcc gaagagaact ggaacctgta tctgaactgg aacaggtcat gattctggaa
    24301 aacatactgc gtgaattacc ttcagatcat gtgcttcgcc gctttcgtga tgacctgtct
    24361 tcagatatca ggcgtctggg caatctgttc cgtatcatga aagaagaaga ctggtctcct
    24421 gaatggatcg caaatgccat tcaggcgtac ctggacagtt tgcctgacag accggaattc
    24481 atctataagg tagcggtgac caaaaaagga gttaaagccg gcgatgtcaa acaggataag
    24541 atagaccagg aaaccgccgc catggaaaaa ctgcaggccg ccgccaacct ctttcccgtt
    24601 tttcaggaaa agatggcaga agcgggccgg ttcgattata gtgatatgat cctctgggta
    24661 gtgcgggctt ttgcccggca tccagacctg ttgcgtacct accaggaaag atttctgtat
    24721 gtacttgttg atgaatacca ggataccaat ggtgcacaga atgaaatact gcgcagcctg
    24781 atcgggtatt gggatgtgcc caatgtgttt gtagtgggtg atgatgacca gagtatctat
    24841 gagtttcagg gtgccagggt gcagaatata ctggacctgt acaggcagta tgaaaatgat
    24901 atgcgaacag tattgctgac agaaaactac aggagcgttc agccggtact ggatgtttca
    24961 cgcatactga tcgatcggaa tgaagaacga cttgtcagcc acctggaagg cctgagtaag
    25021 gtgctgatca gccggggtga agaggtgcgt gacctgacac aacttccggt aatcagagcc
    25081 tatgacagta aggtacagga ggaggcaggg gttttgatgg aaattgaaaa gctgctggaa
    25141 tcaggaacag atcctgccga gatcgcggtc atttattacc ggcatgctca ggcagatcag
    25201 ctgatccggc tgatgggaag aaaacaattg ccctatgcac tcagaaagca attgaacagt
    25261 cttgatgtac cgctggtcag acagatcctg tcggtcatgg agtatgcagg caaagaacac
    25321 cggcagccga acagtgcaga acacctgctt tttgaaatgc tgtatttccc attctttggt
    25381 atccacagac acgacatttt gtctctggct gcttatatgc agggtaaaag gaccaataac
    25441 aactggcgtc aggtaatagc tgatccggat ctaatggccc aggtaaaact ccgtgacccc
    25501 gaggcattgc agaaacttga aagcaggctg accttgtgga tcagtgaagc cggcagcctt
    25561 accctgccta tgctgcttga aaagatcttt gaagaaggag gtgtgctggc ctatatcatt
    25621 gcgcaccatg accgcttctt tttgctggaa gccatgcatg ccttctttga ctatgtgcag
    25681 gcagaatctg ctgccgatcc ggagatgcgg gttgaagatc tcctcgagca actgaacctg
    25741 atgcgcagat atcatattac cctgcctgtc tggcgaacat ttggcaatcg caacggggtg
    25801 cagttcgtta cctgccattc tgctaagggc ctggagtttg accatgtttt tctgataggt
    25861 tgcaacagta atcaatggga atcatccaga agaaacagca ataatttctc gcttccagat
    25921 acccttacat tcagtggtgg ctccaatgaa ctggaagccc agcgcaggct cttctatgta
    25981 gcgctgacca gggccagaaa aggactgcag gtatcttatg ctgaaaccga taacaacgcc
    26041 aaaacactgg aaccttccag gtttgtggaa gagatggctg ttcaggacct tactatccgc
    26101 cgatcgggaa gcctggatga tgaaaaactg gctgcaatag aattgctttc tcttggaaca
    26161 gacaagaccc ctacggcaga actgttcgac ccggactggt tgacccgaaa aaccgaacac
    26221 ctgtccttaa gtgcgtccat gcttaatgct ttcctgaaat gcccggtgcg attttatttt
    26281 cagtatctgc tccgcattcc ccaggccaga aacgacagta tggaatttgg caacgccatt
    26341 cacctggccc ttcagcgcta cttcaatgaa gtcagggaaa caggaaattt tccgccccct
    26401 gaaaaactgg tccaaccgtt cagactgtac atgcaacaac acagggaagc gttcactgat
    26461 aaacaattca gaaacagaat ggagcttggc gagatgatct tacctgaata ttatgcccag
    26521 cacgtgtctt cctggaataa agtggtagtt accgaatatc ctgtcagaga tgtcacatgg
    26581 aagaacatcc ccattaatgg taagctggac aagatagaat tcaatggaaa tgaggtccat
    26641 gtggtggact ataagacagg tagttacagg ctggccaaac aaaaaatgaa agggccaaca
    26701 gataaagatc ctctgggtgg agaatactgg agacaaatgg tcttttacca gatattaatg
    26761 gaccagcaga gacaaaaacc atggcgcatg gtgagtggcg agtttgattt tctggagaag
    26821 aatcctgaca gcggaaaata tgaaaaggaa cgcctggtta tcacttcaga cgataccgcc
    26881 atagtgggca gacagattga agaggtctat ggaaagatcc gcagtttaga tttcacagaa
    26941 gggtgtaatg aacccgattg tgaatattgt aatctgctta aatacgaatg attttgacaa
    27001 agtgttatgc aggtaggttg attacttgct aactttccaa atcaagtggc atcatggcga
    27061 agaaaagttt atatgagcct ttgctggctg aagtggcctg ggaggtgtgt aaccagatag
    27121 gaggaattta cacggtagtg cgaagcaaag caccctacat ctccgaaaaa tggggtgaac
    27181 aatactgcct cattggtcca ttgctggatg acaggcttcc ggcagaattt gaagaagcaa
    27241 ctgatcccga tcccaatgat ccttatacaa aggccgtgga taagctgcgt gcagacgggg
    27301 tggaagtttt cttcggccga tggcttactc agggtactcc gaaagttgta ctcatgcgtc
    27361 cggaacaggc caacgatcaa atgaaagaca tcaggcagta tctgctgaaa caccatcata
    27421 ttaatatttt taatccggat gatctgttgc agcaggtcat cactttcgga catctggtgt
    27481 acagcttttt tgctgccctt gaagaagagt caggcggtag agacatcatt gggcattttc
    27541 acgaatggat gacaggaatg gccatcggtg agatagctcg tgaaaagaga aaaataaaga
    27601 cagtttttac tactcatgct acattattgg gtcgttatgt ggcgatgaac gataccaact
    27661 tttatcgcaa tctgccttcc attgaatggt taccggcagc tcagaagttt tacatagagc
    27721 cacaggtcag gctggagcgg ttagctgcaa acgactgtac cgttttcaca acggtgagtg
    27781 atgttactgg taaggaatgt gagtttctgc tgggaagaaa gcctgacgct attcttccta
    27841 acgggatcaa cctgtatcgc tacatggcta cacacgaact gcaaaacctg catggacagt
    27901 tcaaggagaa gatccatgaa ttcaccatga gtcacttctt ccacagctat acttttgatc
    27961 tcgataatac attgtatttc ttcacctccg gcagatatga atttcataac aaaggctacg
    28021 accttacgct ggaggcgctt cgactcctga atgaaagact gcgaaaggaa aaaacagaca
    28081 tcaccgtggt gatgttcttc attaccaaac agccgtttga gtccatcaat ccgaaagtac
    28141 ttcagtcacg agctgtgatg gatgagatcc agaacacctg tgaggagatc ctgaaacaga
    28201 tcagagagaa attgttctat gcagcagcag ctaatgcaga ccatcacctg cctgcactga
    28261 atgatttcgt ggatgattac tggaagtacc ggtacaggaa aatgatccag tcctggaaaa
    28321 gtgattactg gcctattgtt gttacacaca acctggtgaa tgatgccgct gatgagatca
    28381 tacaatctct gagaaaaaca gatatgatca ataagccgga tgataaggtg aaggtcatct
    28441 accacccgga tttcattaca cctactaacc ctttattcgg aatggactat ccgcagttcg
    28501 ttcgcggttg tcatctggga attttccctt cttattatga accctgggga tacacacctc
    28561 tcgaatgtgt ggcacgtggt gtacctgctg ttacaagtga tctgagtggt tttggcgact
    28621 attgccatcg ccgtttccgt aacctggaag acaagggaat atatattgtc aaaagacgaa
    28681 ataaaacctt tgaagagagc gctcaggatc tggcagagat catgtacagc ttcattcagt
    28741 taaatcgtcg tgataggatc atattgcgaa atacagtaga aagtaatgca gaaaatttca
    28801 gttgggaaaa cctggtggag cattacgaaa aagcctatcg gcaggtgttg gcataaaaaa
    28861 agcacggtac aataccgtgc ttttaaagga tcatttattt tatctgatga gctggaagct
    28921 gccttgacct gagtagctga cccctgtgtt gaacagtgcc tccacaaaat atacataagt
    28981 gcccatttct tgttcttctc cctggtattt tccatcccag ccggcgtaat agtcggcggt
    29041 gttggtttca taaacaagct gtccccagcg attgtaaatg accatgtggt agctattcag
    29101 gttgtatgca atgccttgaa acaggtcatt gacattatcg ccgttaggag taaatgcatt
    29161 gggtaccaat acctcgttgc tgtagatcac agttaccagt atggtgtcaa tagcaaagca
    29221 accgttttca tcagtaatac taacatagta ttcagttgta actacgggtg tgacggtata
    29281 gataaagcaa gggtcatctt cgcagcccaa tacgccgccg tcgggggccc aggtcactcc
    29341 tgtaagatcc agcgtattgg tttcagcagt taaggttgtg ctgaggccat acgtgatcgc
    29401 tacatctgcg ctacctgtaa taaagatctc gtccggttca gtaatggtca gggtggaaga
    29461 ggtggtacaa ccattgtcat cggttacctc taccagataa tctcctgcgc tcagttctgt
    29521 cagaaaactt ccggtggatt ctccgttaac agttatgtca tagggtacag cacctccgct
    29581 cacttctatg gtagcagaac cgttgctgta tccaaaacat tgaacatttt ctacgtcgtc
    29641 aaaaaccaat tccagaggtt ccagttcaat gatgtcaaag tcaaaggtct tttggcatcc
    29701 atccggagca gtaactgtaa cggaatagga gccagctgac aaccctgaaa tggagttact
    29761 tcccgaaaat ccggtggacc aatcataggt gtatggtcct gcgccatcca gtgtgatggt
    29821 agctgcgcca tcggtaagac cgccacaact cacatcggta atatccaggg tgccggagaa
    29881 gttttcagct actgttactg ttacctcgtc tgaatattct gtgccgtcgc acagggtttg
    29941 tactactgta taaacggtgg tttcttcagg agctacatct atagaaggac cattacctac
    30001 cagtgttcct tcatcgtcat accaggaaat gtaagaggtg taccagcgcc aggtttcttc
    30061 agtggcttcc caatcagtgg cgttcctgtc ttctaccgca aacgctattg taccatcttc
    30121 attttgcaac ccttgtgtgg agatgccaag atcccaggta ggacaaacca gtacgttcac
    30181 caggtgattg tctatgaagt tggtggtttc atgtaaaacg atctggaagg taccttcatc
    30241 agaggtgcag gagaacaagg gcacatcttc ccactgtacg atcagcctgc ggttgggtgc
    30301 aactcctacg gtttcataag atacgcatcc tgtacacagg ccggtatgcc agtctgtcca
    30361 ggggccgaat atggcagcct tgggaacatt ggaagcggca tcgggaatag gaccatcagg
    30421 agtccagttg gaggcccatc ctgcaccggg ttctataaaa ctgatccagc cattcgagca
    30481 gatataaaat tctgaataga gctctccaaa gaaacagaat tcaaagccta tgtcatatgg
    30541 acccacatag gtgtcatcaa ccatggttac gtcttcacct gcggtttctt ccggagcata
    30601 ttccagttct tcatatgtgt aacagtcgcc gcaatagaca gaagcatctg agaacagggt
    30661 tactgtttca ccactgcaga tggtggtgtc agggtaagct gtgatctgag cctgcaacac
    30721 aacatatgaa cttataaata gtgcagtaaa tagtagctgt tttttcatat atattctacc
    30781 ggttagcaaa gggcggaata aaattaggca ttttatatgt ccttttgggc gagcctgaag
    30841 tataggagag atcgttgttc tgatgacccg gaagttgtta acaaaggcaa cttcaaacag
    30901 attgcggtcg ttttttaagt agaaggcttc tattatgaca atgctggaat attttaaaat
    30961 gatacttcaa aaggtcagct tcgacctgac actatttgca aaagaatttc tgaaagcagc
    31021 cggaaaatta ccggaagaag aaatgagtga gctcaggatc tggtgccttc aggtttttgg
    31081 cctgcattat tgcagggagg ccgttcctga atttgacagg ggctgacatg cgattgattt
    31141 tgctgtttta agcccgctgg agggatccag ggcttatttg gatgtctgat atctctttat
    31201 gtgttttttt tgattgaagg ggcatttatg aaaacatgta gaaaaagaca aatagatagc
    31261 tataaagaat tgaaaatcat agacttagtt taaaatttag cgtgtattcc cagtcgggtc
    31321 ggatctggac ctcagcctgg taatatagca gcggctctgg ttgtcttttt tcggcaagtg
    31381 atccggttgt ccatttacca tccccatttt catctatgat ggcccgaatg atataggtgc
    31441 ctggcaagag atcttgaagt tgccacaggt agttgtggtc gttccactct ggtattttct
    31501 cggccagcac ttctccagac ttatcgagcc actgaagtat tatcggtctg ttgatggaat
    31561 cagttacatt aacttccagc cttccgtatt ctttgggaga tctgatcctg aatgatctgg
    31621 tgatggtatc attttctatg ccatacagat ctgttaatgc tccgggtagt aaggtcaatg
    31681 tatagatact gtctgccttc cactgagtcg agatgatgag tctgcgcaga tcatttgtat
    31741 ctgtctgaac agaccattct attttttcat caagactatc tgccttcagg atcattctgg
    31801 aactgtcaat ggcggctatt ggacgttgca taagcagctg atatggttga tggagctcct
    31861 gggcggtgaa tcggttcctg ctgcctccgg agggctcagg tacggcggtt tccagcttat
    31921 tatcaaaaaa gttatccgga agtttctttt gggggatgac caggaaggta tccatctcta
    31981 ttgctgatat ccggatcctt tgactatctg tatccgccac ctgcgaccaa tgatacaagg
    32041 tatcgctgcc ggactgaata aatatgcctc cgtgatcctg ttctgtttga taccggaggc
    32101 tgtcgggtgc ctggttgaat atgatgcgca ccaagccggg ctctactgca cgggcatctg
    32161 atacaaaagg cttttgcctc gcttctgtga ataagttcag gtctgcctgt actaccggtt
    32221 gactgaccag cagagagctg tccagaaatg ctatggattc attggggagg tcatagaagt
    32281 agttgaagtt ctgatcctga agtgcataga gattgtatct accctctctt acatagctga
    32341 tccggaagtt tccccgttca ttggttttgg caaaataggt tggtttttgt gtggtgaagg
    32401 cgctgtcttc ggtattgcgg tacaggagta ccaggctgtt tgctgctggt ttgcctgtcc
    32461 atatatcggt taccgttccg ttgatgtata atgagtcgat gacctctccg gtgctaaagg
    32521 cataaacaaa ctggctgtac gggtttcctt ctgttatgtc agcaatactt tcgccaaatt
    32581 gaatggtata agtagtgtta tccaggaggc tgtcaggtag cctgatcaca atgttcttac
    32641 ccttgatttt tatatcggga aattcttcaa gaggaggact gatcagaacc tggttgaagg
    32701 cgtccttcaa ttgtacatac tcatcaaact ttaggattat ggtgcgctct ttcatctgaa
    32761 cactttccat agcggggcta gcttccagca ataccggagg agtattgtcc ttgtcgccac
    32821 ccgtgggagc aaccacattg gcgcagcccc agaggaggcc catcaggggg atcagtagtc
    32881 ttttcatctt cccatataaa ccatcagcac agaaatatca gcaggcgaca ctccgctgat
    32941 gcggctggcc tgaccaagtg ttctgggtct gacccggctt agtttttctc tggcttcgct
    33001 tgaaagtgac tgtagtttat gataatccag gctttctgac agcgatacat cttccaaacg
    33061 ctgcatcttt tcggctattt cctgctcttt ggctacatag ccttcgtatt tgatctcgat
    33121 ctcagcctgt tccaggtatt cttcagaaaa ggctgccagt gctgttttca gatcagggat
    33181 tacagtggca aggtctttta tacctacgtg gggtctggaa atgatggagg tggccttcat
    33241 tttttgttta agtggagcac tgcccaactc ttccagcaac ccgtttacct gatcgggggc
    33301 aacagatagt ttttgaagca ggtctgtgat ggcactggtc tgtgcccgtt tttctatgac
    33361 ctgatggagt cggccgatgt ctgccagtcc tagtgtatgg cttttttctg ttagccgaag
    33421 gtctgcgtta tcctgacgca acagtacccg aaattctgcc cgggaggtaa acatgcggta
    33481 gggctcttct gtacctttat taataaggtc atctatgagt acgccgatat agccttcaga
    33541 tctggatatt tcaaaaggag gaaggtcatg tactgactgg tgggcattga taccggccat
    33601 cagtccctga cacgcagctt cttcatatcc ggtagtacca ttgatctgac ctgcgaaaaa
    33661 gagattcctg accaaccttg tttccaggct atgggttaat tgaacaggag ggaagtaatc
    33721 atattctatg gcatagccag ggcggaacat gcgtacgttt tcgaacccgg ctatctttct
    33781 gagggcttca aattgtactt gttccggcag tgaagtagaa aaaccgttta catatacttc
    33841 tacggtattc cagccttccg gctctacgaa tatctggtgg cggtctttgg aggcgaagcg
    33901 gtctatctta tcttctatgg aagggcagta tcgtggtccc ggacccttga tcctgccgct
    33961 gaacatagga gattcatcaa aacccttgcg gagagcgtca tgcacctcct gagaggtgta
    34021 ggtcacatga cagcttctct ggagctggag cggcttcgta tcagtgtagg agaacttgcc
    34081 cgggtttaca tcaccaggct gttcttccat tttgctgtag tccagactgc ggccatctac
    34141 ccggggagga gtgcccgttt tcattcttcc gctttcaaac cctagttcca ccagctgctc
    34201 ggtaatacca gaagccggtt
//
LOCUS       DAOMAX010000004        32906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_151_length_32906_cov_4.913579, whole genome shotgun sequence.
ACCESSION   DAOMAX010000004 DAOMAX010000000
VERSION     DAOMAX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 32906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151_length_32906_cov_4.913579"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PK742_00590"
     CDS             complement(<1..1129)
                     /locus_tag="PK742_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HQU75113.1"
                     /translation="MKYLPIDQNLFIRNRQAFTGQMKPNAIAVFQSNDEMPSNADQFH
                     PFKQNSDIFYLSGIDQEDSILLIYPDSPNPIYREALFLKKTNEHIAIWEGHKYTEEEA
                     RAASGIQTIHWKEEFPAIFNMLMNHAETLYLNSNENDRASILVETRDMRFAREMQQRF
                     PMHKLERSAPIMARLRVSKSKWEQDLLQTACDITRKAYLRVLQNIKPGMMEYEVEADI
                     THEFLRNRATRHAYGAIIASGASACVLHYVDNNLECKDGDLILMDFGAEYANYCADLT
                     RTIPVNGRFTERQKQVYNACLNVQKKALDMIHAGMILKDYNAEVGKVMESELKAIGLL
                     TDADIKNQDPSWPAYKKYFMHGTGHFLGLDVHDIGNHYEPVP"
     gene            1527..1910
                     /gene="rpsL"
                     /locus_tag="PK742_00595"
     CDS             1527..1910
                     /gene="rpsL"
                     /locus_tag="PK742_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQU75114.1"
                     /translation="MPTIQQLVRKGRETVKYASKSRALDRCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNQTEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTAGVNNRTQSRSKYGTKRPKAAAK"
     gene            1931..2398
                     /gene="rpsG"
                     /locus_tag="PK742_00600"
     CDS             1931..2398
                     /gene="rpsG"
                     /locus_tag="PK742_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQU75115.1"
                     /translation="MRKDKPKKHVIHPDTKYNDPLVTQFVNNLMWQGKKSTAFAIFYD
                     ALERVEKQTGENGYEVWKKALSNSTPGVEVKSRRIGGANFQIPTEIRPDRKISLSIKW
                     LIGYSRKRSGKSMAEKLAAEIVAASNNEGATIKKKEDVHKMAEANKAFAHFRA"
     gene            2429..4537
                     /gene="fusA"
                     /locus_tag="PK742_00605"
     CDS             2429..4537
                     /gene="fusA"
                     /locus_tag="PK742_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQU75116.1"
                     /translation="MASRDLKYTRNIGIAAHIDAGKTTTTERILFYTGLTHKIGEVHD
                     GAATMDWMEQEKERGITITSAATTTFWKYRNEEFKVNIIDTPGHVDFTVEVERSLRVL
                     DGVVALFCAVGGVEPQSETVWRQANRYRVPRIGFVNKMDRSGADFLEVVRQVKTILGA
                     NPVPLVLPIGAEETFKGVVDLIRNVAMIWDDNTQGMTYTEVPIPDDMVEQVQEYRNML
                     VEAVAEYDDALLEKFFDNPDSITEDELVEAIRKATIDIAIIPMLCGSAFKNKGVQFML
                     DAVCRYLPAPIDVEAVKGTNPDTEKEELRRPDAKDPFAALAFKIMTDPFVGRLAFFRV
                     YSGKLDAGSYVLNTRTGKKERISRILQMHANKQNPIEFIEAGDIGAAVGFKDIRTGDT
                     LCVESHPISLESMEFPEPVIGIAIEPKAQADVDKLGIALSKLAEEDPTFKVMFDEETG
                     QTVVRGMGELHLEIIVDRLKREFKLDVNQGAPEVAYKEALTKTVKHRETYKKQTGGRG
                     KFADIQFEIGPADDEVVGLQFVNDIFGGSIPREFIPAVEKGFKEAMGTGVLAGYTMDS
                     MKVRLFDGSFHAVDSDALSFEMAAKIGYRTAARMAGAQLLEPIMKLEVITPEEHMGDV
                     IGDLNRRRGMVEGFDSKNNATVIKAKVPLSEMFGYVTQLRTITSGRATSILEFSHFAP
                     TPTNIAEEVVAKSKGKVAAE"
     gene            4551..4856
                     /gene="rpsJ"
                     /locus_tag="PK742_00610"
     CDS             4551..4856
                     /gene="rpsJ"
                     /locus_tag="PK742_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQU75117.1"
                     /translation="MSYKIRMKLKSFDHTLVDKTAERIIKTVRSTGAVVTGPIPLPTR
                     KKIYTVLRSPHVNKKAREQFELSTYKRLLDIYSASNKTVDALQKLELPSGVEVEIKI"
     gene            4868..5485
                     /gene="rplC"
                     /locus_tag="PK742_00615"
     CDS             4868..5485
                     /gene="rplC"
                     /locus_tag="PK742_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQU75118.1"
                     /translation="MKGIIGKKVGMTHYFDNDGRSVACTVIEAGPCVVLQKKTIETDG
                     YDAVQIGFDEKKEKNTSAGMKGHFKKAGAEPVKQISEFRDFNTELNVGEHLTVDMFTE
                     GEKVHVKGTSKGKGFQGVVKRHKFSGVGMRTHGQHDRHRAPGSIGGSSWPSRVFKGMR
                     MAGRMGNETVKTRNIKIAKIISEQNLILLNGSVPGSKGSYVIIEK"
     gene            5488..6117
                     /gene="rplD"
                     /locus_tag="PK742_00620"
     CDS             5488..6117
                     /gene="rplD"
                     /locus_tag="PK742_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQU75119.1"
                     /translation="MKLDVVNSAGQKTGRQVELSDAIFGIEPNDHSIYLSVKQYLANQ
                     RQGTHKSKERSEVAGSTRKLHRQKGTGGARKGDTKNPIFYGGGTIFGPKPRNYSFKLN
                     RKVKELARRSALSYKAKNNEIIVIETPEMDKPSTRTYAGMLKGIGADGGRSLVVLSES
                     NVNVVMSGRNIQRTEVATAADLNTYAILKAKHLVLTEQSVAAINQMAEQ"
     gene            6128..6427
                     /gene="rplW"
                     /locus_tag="PK742_00625"
     CDS             6128..6427
                     /gene="rplW"
                     /locus_tag="PK742_00625"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQU75120.1"
                     /translation="MSSKRVLIKPLITEKLTQLSEGLRTYGFQVDPKANKIEIKQAIE
                     KMYGVQVEEVRTMNSIGNYGWQRTPFGISKGSKGQMKKAYVTLKEGEEIDFFSNV"
     gene            6436..7260
                     /gene="rplB"
                     /locus_tag="PK742_00630"
     CDS             6436..7260
                     /gene="rplB"
                     /locus_tag="PK742_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQU75121.1"
                     /translation="MAVKKYNPVTPGTRFRVGNAYAEITTNVPEKSLLAPAKSTGGRN
                     SSGHMTVRYRGGGHKQKYRIIDFKRSKHDIPATVATIEYDPNRSAFIALLNYKDGEKR
                     YMLAPNGLAVGQEVLSGDSVAPEVGNALMLKNMPLGTIIHNIEMYPGKGGMIARSAGT
                     WAQLTAKEDKYAVVRMPSGETRKILLACYATVGTVSNPDHNLQSLGKAGRKRWLGRRP
                     HNRGVVMNPVDHPMGGGEGRSSGGHPRSRTGLYAKGKKTRHPKKNSSKFIISKRKK"
     gene            7268..7531
                     /gene="rpsS"
                     /locus_tag="PK742_00635"
     CDS             7268..7531
                     /gene="rpsS"
                     /locus_tag="PK742_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQU75122.1"
                     /translation="MSRSIKKGPYIDHNLAGKVESMNASGKRTVIKTWSRRSTISPDF
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFSPTRNFKGHSGNR"
     gene            7543..7929
                     /gene="rplV"
                     /locus_tag="PK742_00640"
     CDS             7543..7929
                     /gene="rplV"
                     /locus_tag="PK742_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQU75123.1"
                     /translation="MEAVAKLINNPRTNNNAGSPRKARLVIDSIRGLDIDKALNILRF
                     TNKSAAAPVEQVLRSAINNWEQKYGMDAAENNLYVKSCFIDGGKTMRRYLPAPHGRAY
                     RMRKRTNHITIVVASRNEQQNDSNEK"
     gene            7937..8644
                     /gene="rpsC"
                     /locus_tag="PK742_00645"
     CDS             7937..8644
                     /gene="rpsC"
                     /locus_tag="PK742_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQU75124.1"
                     /translation="MGQKANPIGNRLGIIRGWDSNWFGGKNGAEKLIEDEKIRKYLDA
                     RISKGGIARVVIERTLNRITITIHTSRPGIIIGKGGGEVDKVKEELKKITQKEVQINI
                     VEIRRPELDANIVAETIAKQLEARINFRRAIKMAVASTMRMGAEGVKIRVSGRLNGAE
                     MARSEEYKQGRVPLHTFRADIDYAWKEALTVYGKIGIKVWICKGEVFGKRDLSPNVGT
                     QQERQRKRPGRGMAKRG"
     gene            8660..9079
                     /gene="rplP"
                     /locus_tag="PK742_00650"
     CDS             8660..9079
                     /gene="rplP"
                     /locus_tag="PK742_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQU75125.1"
                     /translation="MLQPKRVKHRRVHKGRMKGNAGRGNQLSQGSFGLKTLEEAWITD
                     RQIEAARVALTRHLKREGQVWINIFPDKPVTKKPAEVRMGKGKGAPEYWAAVVKPGRI
                     MFEVDGVPMELALEAMRLAAQKLPVATKTVIRRDYTE"
     gene            9091..9297
                     /gene="rpmC"
                     /locus_tag="PK742_00655"
     CDS             9091..9297
                     /gene="rpmC"
                     /locus_tag="PK742_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQU75126.1"
                     /translation="MAKIKSEDLKSLNVQDLQQRLEDDRERLQKLQFNHAVNPLENPM
                     QVRFLRREIARLQTEIRKRELAEN"
     gene            9303..9563
                     /gene="rpsQ"
                     /locus_tag="PK742_00660"
     CDS             9303..9563
                     /gene="rpsQ"
                     /locus_tag="PK742_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQU75127.1"
                     /translation="MEETRNLRRTIQGVVTSNKMEKGITVTVERKVKHPKYGKFVKKS
                     KKYHAHDESNDANVGDVVRIMETRPLSKLKRWRLVDIIERAK"
     gene            9565..9933
                     /gene="rplN"
                     /locus_tag="PK742_00665"
     CDS             9565..9933
                     /gene="rplN"
                     /locus_tag="PK742_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQU75128.1"
                     /translation="MIQQESRLKVADNSGAREVLCIRVLGSTGRRYASLGDKIIVTVK
                     DALPGGGMKKGTVSTAVVVRTNKEVRRKDGSYIRFDDNACVLLNASDEPRGTRIFGPV
                     ARELRDKQFMKIVSLAPEVL"
     gene            9944..10270
                     /gene="rplX"
                     /locus_tag="PK742_00670"
     CDS             9944..10270
                     /gene="rplX"
                     /locus_tag="PK742_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQU75129.1"
                     /translation="MSRFKPKYHVKKDDLVEVIAGEDKGKKGKVIEIIAGKDRVLVEG
                     INIISKHTRPTAQYPNGGIIKKEAPIHISNVLLVNPATGKGGRTSVKVENGKKERIFK
                     TAKNSK"
     gene            10270..10818
                     /gene="rplE"
                     /locus_tag="PK742_00675"
     CDS             10270..10818
                     /gene="rplE"
                     /locus_tag="PK742_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQU75130.1"
                     /translation="MSYVPRLKNKYLDEVVPQLKEKFGYKNVMQVPKLVKISVNQGVG
                     AATQDKKLVDVAVDEMSRITGQRAVPTMSKKAISNFKLREEMPIGARVTLRGERMYEF
                     LDRLIAVALPRVRDFKGVNNKSFDGRGNYTLGITEHIIFPEIDIDKINKVTGMDITFV
                     TTAETDAEAFELLKGLGIPFKK"
     gene            10822..11091
                     /gene="rpsN"
                     /locus_tag="PK742_00680"
     CDS             10822..11091
                     /gene="rpsN"
                     /locus_tag="PK742_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQU75131.1"
                     /translation="MAKKSIIARNDKRERLVALYAERREQMKNDGRWEDLDKLPRNSS
                     KVRLRNRCQLTGRPRGYVRDFGICRNQLREMALYGKIPGMKKASW"
     gene            11111..11512
                     /gene="rpsH"
                     /locus_tag="PK742_00685"
     CDS             11111..11512
                     /gene="rpsH"
                     /locus_tag="PK742_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQU75132.1"
                     /translation="MKTTDPIADFLTRIRNAQQANHRVVEIPASNLKKRMTEILYENG
                     FIYRYKFDDNGPQGTIKIALKYDPVSKVPAIRGLERVSRPGLRHYQHVDGLPRVLSGL
                     GMAIVSTSKGVMTEKEARRQNVGGEVLCYVY"
     gene            11534..12088
                     /gene="rplF"
                     /locus_tag="PK742_00690"
     CDS             11534..12088
                     /gene="rplF"
                     /locus_tag="PK742_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQU75133.1"
                     /translation="MSRIGKAPIALPEGVTVNVSDKNLVTVKGKQGELTQQVDRDIIV
                     KVDGNQLVVERPTDQKRHRAMHGLYRALIANMVKGVSEGFKKQLELVGVGYRASHQGQ
                     ILELSVGYSHPIMVVLPKEIKLNTISEKGKNPTVVLESSDRQLLGQVSAKIRSLRKPE
                     PYKGKGIRYSDEVLRRKAGKTAGK"
     gene            12100..12450
                     /gene="rplR"
                     /locus_tag="PK742_00695"
     CDS             12100..12450
                     /gene="rplR"
                     /locus_tag="PK742_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQU75134.1"
                     /translation="MQNKKAQRRRKIRYGIRKKIAGTADRPRLAVFRSNKQIYAQIID
                     DVAGHTLVAASSRELTDAKGNKTEIAKMVGTELAKKASEKGIASVAFDRGGFLYHGRI
                     KALADGAREGGLTF"
     gene            12460..12981
                     /gene="rpsE"
                     /locus_tag="PK742_00700"
     CDS             12460..12981
                     /gene="rpsE"
                     /locus_tag="PK742_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQU75135.1"
                     /translation="MATNKEIQRVRPNETELTEKIVGIQRVAKVTKGGRTFSFSALVV
                     VGDGQGTVGHGLGKAREVTEAIQKGIDDAKKNLIKVPVHKGTIPHEQTGKYGAGKVML
                     KPAAHGTGVIAGGAMRAVLESAGVTDVLAKSQGSSNPHNVVKATLDALSKLRTPMAVA
                     AQRNVSLQKVFKG"
     gene            12986..13165
                     /gene="rpmD"
                     /locus_tag="PK742_00705"
     CDS             12986..13165
                     /gene="rpmD"
                     /locus_tag="PK742_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQU75136.1"
                     /translation="MEKLKLTAVKSTIDRPAKQKATVKALGLRKLNQTVEVEATPQVL
                     GMIEKVKHLLKVEKA"
     gene            13168..13617
                     /gene="rplO"
                     /locus_tag="PK742_00710"
     CDS             13168..13617
                     /gene="rplO"
                     /locus_tag="PK742_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQU75137.1"
                     /translation="MDLSNLKPAKGSVRKNKRIGRGQGSGHGGTSTRGHKGANSRSGN
                     KKKHHFEGGQMPLQRRVPKRGFKNPNTVEYTTLNLDQIQEMADKYGMTGFDQEILMAN
                     GVIGRNDKLKVLGRGELKAKVDIHAHAFSKTAKAAIEALGGTANVIG"
     gene            13621..14982
                     /gene="secY"
                     /locus_tag="PK742_00715"
     CDS             13621..14982
                     /gene="secY"
                     /locus_tag="PK742_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQU75138.1"
                     /translation="MKRLIETLKNIWHIEDLRKRILYSIGLIFIFRLGSYVILPGIDA
                     DLYKQAVEGGASGGLLGLINLFAGGGFSRASVFALGIMPYISASIAMQLLTIAVPSFA
                     RLQKEGESGRRKINQYTRWLTVVVTAFQASGYYIYLKSLSEGNGGIDFIVTSNGMFWF
                     ICMIILTTGTLFTMWLGEKITDRGVGNGISFLIMVGIIASLPASLINEFTSRVNENAG
                     GLVPFVLELGFLVVVVMGCILLVQGTRRIPVQYAKRVVGTRQYGGNRQFIPLKINAAG
                     VMPIIFAQALMFIPGTVQQFFPDSQSNFIMQLNDFTSVTYNIIYGFLVIIFTYFYTAI
                     TINPTQIADDLKKNGGFVPGVKPGKKTAEFIDNVLSRITLPGSIFLALVAIMPVFTTA
                     FGVGSQFARFFGGTSLLIMVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRSSGAQTP
                     VGM"
     gene            14985..15800
                     /gene="map"
                     /locus_tag="PK742_00720"
     CDS             14985..15800
                     /gene="map"
                     /locus_tag="PK742_00720"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQU75139.1"
                     /translation="MIYYKTSDEVELIRKSSLLVGKTLAAVAAAIKPGMTTLELDTLA
                     ETVIRDHGAVPSFKGYNGFPGTLCVSVNEQVVHGIPGNRVIQDGDVVSVDCGAFMNGF
                     HGDSAYSFLVGNVPAAAVRLAKVTKESLYLGIGKAVAGNRIGDISHAIQSYCETQLGY
                     GVVRELVGHGIGRELHEAPEVPNFGTRGRGVQMQEGLVIAIEPMINLGGRQVVQEKDG
                     WTIRTRDNKVSMHYEHTVAVGKEKADILSSFEEIEEAVSKNEYLITTEEVTEQ"
     gene            15800..16018
                     /gene="infA"
                     /locus_tag="PK742_00725"
     CDS             15800..16018
                     /gene="infA"
                     /locus_tag="PK742_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQU75140.1"
                     /translation="MSKQGVIKQDGVVTEALGNAKFRVKLENEHEILATISGKMRMHY
                     IRILPGDRVTVEMSPYDLTRGRIIYRYK"
     gene            16028..16144
                     /gene="ykgO"
                     /locus_tag="PK742_00730"
     CDS             16028..16144
                     /gene="ykgO"
                     /locus_tag="PK742_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HQU75141.1"
                     /translation="MKVRPSIKKRSADCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            16156..16536
                     /gene="rpsM"
                     /locus_tag="PK742_00735"
     CDS             16156..16536
                     /gene="rpsM"
                     /locus_tag="PK742_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQU75142.1"
                     /translation="MARIAGIDLPKNKRGVIALTYIYGIGDTTAAKILTTAGVDLSKK
                     VKDWDDDEVNAIRNTINNEYKVEGALRSEVQLSIKRLMDIGSYRGVRHRKGLPVRGQQ
                     TQSNARTRKGRKKTVAGKKKAPKG"
     gene            16566..16958
                     /gene="rpsK"
                     /locus_tag="PK742_00740"
     CDS             16566..16958
                     /gene="rpsK"
                     /locus_tag="PK742_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQU75143.1"
                     /translation="MAKSSKKTTKKRVVKVDADGQAHIQASFNNLIVTFTNGSGQTIS
                     WASAGKAGFRGSKKNTPYAAQVAVSDAAKVAYDAGLRRVDVFVKGPGSGREAAIRQIA
                     NSGIDVMVIKDITPLPHNGCRPPKRRRV"
     gene            16987..17589
                     /gene="rpsD"
                     /locus_tag="PK742_00745"
     CDS             16987..17589
                     /gene="rpsD"
                     /locus_tag="PK742_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQU75144.1"
                     /translation="MARYTGPTSKKARSFGEPIFGFDKAFEKRKYPPGQHGVSKRRKS
                     PSEYGVQLKEKQKAKYTYGVLERQFAKTYEEATKRHGVTGEVLLQLLEARLDNTIYRM
                     GLAPTRRAARQMVNHKHIEVNGMVSSTPSHSLRPGDVITVRERSKDMKIIREANPDSR
                     KYNWLEWNPEQQRGVFLQYPERDQIPETIKEQLIVELYSK"
     gene            17616..18614
                     /locus_tag="PK742_00750"
     CDS             17616..18614
                     /locus_tag="PK742_00750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQU75145.1"
                     /translation="MSILTFQRPDKITLQKSTDFEGTFEFKPLEPGFGVTVGNALRRV
                     LLSSLEGFAITSVKISGVDHEFSTISGVMEDVTEIILNLKQVRLKRVMGSDENQVEKI
                     FISLKDKEEFKAGDIEKHTNGFTVMNPDLLICRMESVVKLDVELTIQKGRGYVPAEEN
                     NLLEQTIGVIPIDAIFTPIKNVRYTIENTRVEQKTDYEKLLIEIKTDGTIHPEDAIKE
                     AARILIQHLMLITDENITFHTSVEKEVDAVDEHTLHIRKLLKTPLEDLDLSVRAYNCL
                     KAAKINNLSELVKYDTNELLKFRNFGKKSLVEIEAMVIDKGLQFGMDLSKFKLEED"
     gene            18641..19180
                     /gene="rplQ"
                     /locus_tag="PK742_00755"
     CDS             18641..19180
                     /gene="rplQ"
                     /locus_tag="PK742_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQU75146.1"
                     /translation="MRHGNKINHLGRTFSHRKAMLSNMATSLIMHKRIHTTVAKAKEL
                     RKFVEPIINRSKEDTTNNRREAFAALQSKEAVSELFNHIGGKVANRNGGYTRILKVSD
                     NRAGDNAEMCYIELVDYNEAMLKEAKGPAKKRTRRGTAKTAAPKAEKKEEVTAAAEEA
                     VEEIKPEAEDAAADSEEQA"
     gene            complement(19249..20130)
                     /locus_tag="PK742_00760"
     CDS             complement(19249..20130)
                     /locus_tag="PK742_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75147.1"
                     /translation="MTVTKTTLRRISRKKAYSAAALTFLAAGGAEAQVVLTDIDPDVT
                     YSAPDGVFYLDLDNDGTEDFLFSATSAAAFVIGSGGTNQYAINGVFASPYGSNEILVN
                     SVFSGFAYPAVLNSMDMIGSSDLFAAVSFQSLAYSFQYSSGGGLSPVEQNGNWLGGTD
                     GKFIGLKLHKDGADHYGWLRLDVGSDNKSFTVSMYAYEATPGQGITVISTGIQDAVLT
                     NWTVYSYGSMIHLIQNSTAETGTYQVLDLSGRTLLSGNMDGTHTTVMANQLPAGIYVV
                     RLETASGTLSRKVYVNQ"
     gene            complement(20142..21056)
                     /locus_tag="PK742_00765"
     CDS             complement(20142..21056)
                     /locus_tag="PK742_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75148.1"
                     /translation="MKKKNTPSLLGYSALAATFVAVGSEAEGQVIYTDIDPDVTIDLN
                     GDFFELDLNGDATPDFRLTKRFLSSFNPTIYTTYGNVYSGTIQLNHIYGTALGSNAIL
                     GDITTFGFQYPFALDSLAWLCEDGEWMEGGYQSLVYSYGYKLNLGSSVVSYVQLNGDG
                     FWFGGQTDKYLGLQLRQDGEHYFGWLRLDVAPDNIAFTVKEYAISAVPDSCIQTGHPA
                     PDAITGVWPTGWNAYTWNNRLVIHTGNDVTGAPFRLVDLTGREVLSGRIDQPLLQIDT
                     DLPAGIYNIMIQFNDLILQRKLYLSAAF"
     gene            complement(21153..22067)
                     /locus_tag="PK742_00770"
     CDS             complement(21153..22067)
                     /locus_tag="PK742_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75149.1"
                     /translation="MNNESAFSTKLAAYSAMAGATLLGAGSTFGQNVIQYTDVDPDIT
                     LEMQGDLFNLDLDADGTTDFVIYKYFSAYPYIFSTASGSSVSGTILFNHLFALPATGN
                     AMSGSYGYSGYPYPFALNAGQSVSNASQMIPAGLQSLVYSRYYSVEASGLSVSNVQRG
                     NWLGGKTDKYLGLQFMIGEETHYGWVRLDVAADHTTCTIKDYAYNTIPDMAIATGQNE
                     LVPVLEYSAIGQTEIYSFGQTVVIQTPEAPAAEATVHITGIQGQVVFSGNLVPSGMQI
                     HLDQAAAGVYLVQVETAGFRLTRKVFIE"
     gene            complement(22204..23070)
                     /locus_tag="PK742_00775"
     CDS             complement(22204..23070)
                     /locus_tag="PK742_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75150.1"
                     /translation="MKKIYNESASKKLAAYSAMAATMVATQADAQTIVYEDIDDVTIE
                     IGDIVLVDVNGDGYDDFLFQATSSTGGVWSFGRVFGNVPSYSVGAVSNKVMGYSGAIL
                     PYGSALDAGALVSASADFLGDSSSNPGNVAFLNSIYSGATYGQFADVTDKYLGFSFDV
                     DGNTHYGWMRLDGTVGPVTLTIKDLAYNSTAGESIETGDMGDVVQLSLIDASQLTAYS
                     FGNTIHINVNGLEANNAQVQVSNALGQVVYNNNLDMSGMDIVLNNAATGVYSIRIVAD
                     GAVFSKEVFIGQ"
     gene            complement(23163..24047)
                     /locus_tag="PK742_00780"
     CDS             complement(23163..24047)
                     /locus_tag="PK742_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75151.1"
                     /translation="MKLKNDQQLTGRLAAYSALSSLLLWSADASAEVVYQNIDPDLLV
                     NPGDTAYLDLDGDGTDDLMFWIRSQQGMISTAEGGSFSYTYRMAYASGLNNNVIFGEP
                     GSSGGYEFLMPYNFSSGAFIADTLELFPGPARLAAFVQLDGIPFVSSGPWNGLSGMLG
                     MRFKIGDDSHYAWIRLSVGMAATSITIEEYAWDDQANIGGITAGITAPVPEPALASAI
                     QCWTDGQAVLVQRPEEWQVHYSLFDLQGRLIPFTQQAGQPDRLLPATAVQDGIYLVQI
                     SSAEHGTATRKIFLASGF"
     gene            complement(24031..26592)
                     /locus_tag="PK742_00785"
     CDS             complement(24031..26592)
                     /locus_tag="PK742_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQU75152.1"
                     /translation="MGKRLAKKVLLIGWDAADWKVINPLMDAGHMPTLEKLVNGGVMG
                     NLATLDPPLSPMLWTSISTGKRPYKHGIHGFTEPDPSGKGVRPAFITSRKVKAVWNIF
                     NQHHMKSHVVGWWPSHPAEPINGIMVSNFFQKATKPIHEPWPMAPGTVHPENLAGIFE
                     KFRVHPGELSAAHLLPFVPDAAKVDQEKDKRLQMLAKVIADASSIQAAATYILENEEW
                     DFVGVYFDAIDHFCHGFMKFHPPQLPGVPDDLYQLYKDVVISGYKFHDMMLERLLELA
                     GDDVTVMLISDHGFHPDHLRPLRLPKEPAAPALEHSPYGIVVINGPNIKKDERIYGAS
                     ILDVTPTLLTLFGLPVANDMDGNVMINAFEEQITPDRIDSWENIPGPSGQHPADRQED
                     PYAAHEAMEQLIELGYVERPDENAEKAIQKTVAENNYYLARAYIHGRKTEEAVQILEG
                     LVKENPDVNRYAMRLAHCYQSTGKIAECRTVLKNLKEATGSESVGMQVMEGGLLLMEN
                     KPKEATEIFEAIEKEHVGARVGLQLGKSYLLLKNWKKAEAAFRKELEHDPGNASAWHG
                     LGVSLLRQGNYEEAADCFLNAVGFIYHFPFAHYHLGETLYHMGEYERSAEAFEVTLKM
                     APGVNKARQWLSRIYREHLNKPELAEQKTEAMEDHMRGDVVVVSGLPRSGTSMMMQML
                     DKGGLEIFSDKKRTADENNPKGYYEHEAVMSISKNKAWLEEAKGKVVKVISHLLFELP
                     ARYNYKVVFMERDLNEVLMSQHRMLVRNGKAKEDTINLKLMGPFRQNLEKVRNWATNQ
                     ANVDILYVSHAAMMNNPGEEISRINQFLGGKLDTAAMAGVIDRSLYREKANETKE"
     gene            complement(26592..26813)
                     /locus_tag="PK742_00790"
     CDS             complement(26592..26813)
                     /locus_tag="PK742_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75153.1"
                     /translation="MENKKEVKQITITTKDGKPFAVPPEGSLGLLALGHVGLMAWRQS
                     RAAYAEKMKELQAAGKLPQQTEQEQKKQD"
     gene            26909..28414
                     /gene="lysS"
                     /locus_tag="PK742_00795"
     CDS             26909..28414
                     /gene="lysS"
                     /locus_tag="PK742_00795"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQU75154.1"
                     /translation="MALSEQEIIRRESMHALRQMGIEPYPAAAVEVNTNSRDILDRFD
                     SEPEAFAGVKLAGRLMSKRIMGKASFAVIHDQEGPIQIYIARDEICPEENKDLYNEVF
                     KKHMDIGDIIGVAGHVFKTKTGETTVHVTELKMLSKSLRPLPIVKTDSEGQVHDAFTD
                     PEQRYRMRYLDLIVNPHVRETFVKRTKMVNAMRDYLNERGYLEVETPVLQPLYGGAAA
                     RPFKTHHNTLDMTLYLRIANELYLKRLIVGGFDGVYEFSKDFRNEGMSRFHNPEFTQV
                     ELYVAYKDYEWMMDLVEEMVERVAIAVTGGTIVQVGEHAIDFKRPWTRYTMFGAIEHF
                     TGVDISGMDEKALYQAAKDMHVHVDASMGKGKIIDEIFGEKCEPHLIQPTFITDYPVE
                     MSPLAKKHRTKEGLVERFEAVVNGKEICNSFSELNDPIDQRHRFEAQLELGKRGDEES
                     MLLDEDFLRALEFGMPPTAGLGIGIDRLAMIMTNSQSIQDVIFFPQMRPEK"
     gene            complement(28538..29863)
                     /locus_tag="PK742_00800"
     CDS             complement(28538..29863)
                     /locus_tag="PK742_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75155.1"
                     /translation="MRSVLSLLMTVALSASVAYGQFNIIVEDDFTRPPTTTELGTTMN
                     GLSWFEGGGLRGFVSGDELVFPSEGVTDRDWAFVNLTSVSPFYVSTMTDNTAPIVWTF
                     NMQSDAASLSGVGTSQNGMAYVLASNNQNFESGNGYAVVLGNPGSLDRLRLVKYNDGL
                     DNGTNLTNIVKGGDFLNEYLAVKVIYDPVSDNWTLQYASSDVSFPDPGTASYSTIGSS
                     VDNTYTGSSGFLYTGPMYSHSALTSEMRFDNLLIIQEWVLPITLETFEAYVAENDVTL
                     NWVTGTEINNDFFTLEHSMDGSHFEAIGTIAGNGTTTDAHQYSFLHEDLFAGTHYYRL
                     VQNDYNGLQHFSNVITARISAGFAHTSVSPNPVFDVMQVSLVQSAAQEGTIYIMNLQG
                     QVIMQQDVSLKTGQQVITLNTGDLDAGTYLMQLMGNNGESTSLRFVKNK"
     gene            30155..31354
                     /locus_tag="PK742_00805"
     CDS             30155..31354
                     /locus_tag="PK742_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75156.1"
                     /translation="MRTFQLTLLAVALGFSGAVAAQSLSAGDIAIVQMNADGTDNWAF
                     VATTDIPGGEMISFTDNGWKADNTFRTGEGIITWTAPEEGVACGTVVTIDYNALASVG
                     SASESGSLDFSSSGDQLLAYQGSTEAPVFLAALNNDGSAVWQSDATSSNTSALPMGLT
                     NGVNAGAVNELDNVASTIASISDTRANILSTVYDPANWAGDDTNPQTFAGTVEVTECA
                     PPPSCPVTTGLLASDITLNSAVLSWDDMGPDVQGYKFQLQNIAEGTNRRKTLDAGTTS
                     LFLGPLVLSPGSDYVFAVKTLCLDGSQSAWSELYYFSTASAERMTESSMNLSVYPNPS
                     NGSFYLNGPVTNGAQLIITDLSGKTVYTQVLDGSAELLQTDLAAGMYHLVILGGDETF
                     HTSLVIE"
     gene            complement(31423..>32906)
                     /locus_tag="PK742_00810"
     CDS             complement(31423..>32906)
                     /locus_tag="PK742_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75157.1"
                     /translation="NGLSLEVADASDSLLVGGSVVIESGGSLLNEHSGLIAVDGDLQI
                     DAGGFLSLYFSFLELKGDYANLSSAADFDETISFITLLGSADQTISTSGFEEQFFYLI
                     INKPAGQVNLSNDLAISDGLVFASSTLINTFANTLYINSIFSATISGYGTNAYVNGNL
                     KRDVSAGATYTFPVGTAAQYEEAVIQITSAVGLNNITVSFDPTDPVGPVGLFSEGPVV
                     DGIVTPVIDFLNYGYWNIDPDAVTSVTYSITLTSRGHTNAAPYLDFHGIFKNSNALSA
                     TGWVEPGVVADLPPLITSPTDPVTVTNFGATSFSDFAIGFSELYPLPIVLTNFSADNQ
                     KEEVILHWTTSSEINNDHFTVERSGDGVYFEPVGTVAGAGNSNTALHYQLTDDEPLPG
                     TSYYRLKQTDFDGQHTWSEIVPVQRLTGSVELTVYPNPAHDILYIPAVAGTVSIFAAD
                     GQLVWQATGNGQMQVQVSDWAHGTYMMRITQSSGTTEQMFLVQ"
BASE COUNT         8865 a         7937 c         8488 g         7616 t
ORIGIN      
        1 ccggaacagg ctcgtagtga ttgcctatgt cgtgcacatc aaggcccaga aagtgacctg
       61 tgccatgcat gaaatacttt ttatatgccg gccaggaagg atcctgattt ttaatatctg
      121 catcggtcag cagtccgatg gctttgagtt cactttccat gaccttaccc acctcggcgt
      181 tgtagtcttt caggatcatg cccgcatgga tcatgtccag cgccttcttc tgtacattca
      241 gacaagcatt gtacacctgc ttctggcgtt cggtgaaacg tccgttcacc ggaatggtgc
      301 gggtcaggtc tgcacagtag ttggcatatt cggcaccgaa gtccatcagt atgaggtcgc
      361 cgtccttaca ttccaggttg ttgtccacat agtggagtac acaggcgctt gccccactgg
      421 cgatgatggc accataagcg tgtctggtgg cccggttacg cagaaattca tgggtgatat
      481 ctgcctccac ctcatattcc atcatacctg gtttgatgtt ctgcagaacc cgcagatagg
      541 cttttctggt tatatcgcag gctgtctgca gcagatcctg ttcccatttg cttttgctca
      601 cccggagcct ggccatgatg ggggcagagc gctccagctt atgcatggga aatcgttgct
      661 gcatttccct ggcaaagcgc atatcgcggg tctctaccag gatactggcc cggtcatttt
      721 cattgctgtt gaggtacaat gtttccgcat ggttcatcag catattgaag atggcgggga
      781 attcttcctt ccagtggatg gtctggatac cgctggccgc tctggcttct tcttcggtgt
      841 atttgtgccc ttcccagatg gcaatatgct cattggtctt cttcaggaac agtgcctccc
      901 tgtaaatggg gttggggctg tccgggtaga tcagcaggat gctgtcttcc tggtcaatgc
      961 cactcaggta gaatatatcg ctgttctgct tgaaaggatg gaactggtcg gcattggagg
     1021 gcatttcatc attgctttgg aaaacggcaa tggcgttggg tttcatctgg ccggtaaatg
     1081 cctgcctgtt gcggatgaac aggttctggt ctatgggtaa atacttcata ataacaggag
     1141 cttattcgtc aggaggccaa agatacgatt ttagccatcc gtggccagga aacggaaaat
     1201 cagcaaaaaa atgagacaat tgtatggtta tcaattattt atctaaatgc agaagacaga
     1261 cagggaaaga ggcttgcaaa aggcagaaaa tctcctgtaa attatttctg acgcggattt
     1321 tgcacattca tttcttacag ttacctttgc agtccgtttt ttacaggcgg caggtaaatt
     1381 aagggagtgg accccgataa cgttcacaat atcaggacct gcatgaatcc cggaacaccg
     1441 ggactgctat gctcacctct tattgtggat catttttccc tcctacgatt caccggtagc
     1501 ctacaaaact taatgtaaaa gaagatatgc caacgattca gcaattggtt cgcaaaggcc
     1561 gggaaaccgt caagtatgcc agcaaatcca gggctctgga ccgctgcccg caacgtcggg
     1621 gagtttgtac cagggtctat accactacac cgaagaagcc gaactcggcg cttcgtaaag
     1681 tggctaaggt gaggctgacc aaccagaccg aagtgatcgc ctacattccg ggagaaggac
     1741 acaaccttca ggagcactct atcgtgctca tccgcggtgg cagggtgaaa gaccttcccg
     1801 gtgtacgcta tcacatcgta aggggtgccc tcgatacagc cggcgtcaac aaccggaccc
     1861 agagccgctc caagtatggt accaagcgtc cgaaagcagc agcgaaataa tcaagtaagc
     1921 gaacagtatc atgagaaagg ataaacctaa aaagcacgtc atccacccgg ataccaagta
     1981 caacgatccg ctggtgaccc aattcgtgaa caatctgatg tggcagggca agaagagcac
     2041 tgccttcgcc atattctacg atgccctgga aagagtggag aagcagaccg gagagaatgg
     2101 ttatgaggtt tggaaaaaag cactcagcaa ctccactccg ggtgtggaag taaaaagccg
     2161 ccgtatcggt ggtgccaact tccagatccc cactgagatc cgcccggaca ggaagatctc
     2221 tttgtccatc aaatggctga tcggatacag ccgcaagcgt tcaggtaagt ccatggctga
     2281 aaagctggct gccgagatcg tagccgcctc taataatgaa ggcgctacca ttaagaaaaa
     2341 agaagacgtt cacaagatgg ccgaagccaa caaagccttc gcccacttca gagcctaata
     2401 aatcccagca caatagcaga tatagtatat ggcaagcaga gatcttaaat acacgagaaa
     2461 tatcgggatc gccgcccaca ttgacgccgg taagaccacc acgaccgagc gtatcctctt
     2521 ttacacgggt ctgacccaca agatcggcga agtacacgac ggtgctgcca ccatggactg
     2581 gatggagcag gagaaggaac gtggtatcac gatcacttcc gctgcaacga ccaccttctg
     2641 gaaataccgc aacgaagaat tcaaggtcaa catcattgat actccgggtc acgtggattt
     2701 caccgtagag gtggagcgtt ccctccgtgt actggatggt gtggttgcct tgttctgtgc
     2761 tgtaggtggt gtggagcctc agtctgagac cgtttggcgt caggctaacc gctaccgggt
     2821 tccccgtatc ggtttcgtga ataagatgga ccgttccggc gccgacttcc tcgaagtggt
     2881 tcgccaggtg aagaccattc tcggtgccaa tccggtgccg ctggttcttc ctatcggtgc
     2941 agaagaaaca tttaaaggtg tggttgacct gatccgcaac gtagcgatga tctgggatga
     3001 caatacccag ggtatgacct ataccgaagt gcctatcccc gacgatatgg tggagcaggt
     3061 gcaggaatac cgcaacatgc tggtagaggc tgtagcagaa tatgatgatg ccctgctcga
     3121 aaaattcttc gacaatccgg acagcatcac cgaagatgaa ttggtggaag ccatccgcaa
     3181 ggcaaccatc gatatcgcta tcatcccgat gctttgtggt tctgccttta agaacaaggg
     3241 tgtacagttc atgctggatg cggtttgccg ctacctgccg gctcccattg atgtggaagc
     3301 tgtaaaaggt accaacccgg atacagagaa agaagagctc cgtcgcccgg atgcaaaaga
     3361 tccgttcgct gcacttgctt tcaagatcat gaccgatcca ttcgtaggtc gtctggcatt
     3421 cttccgggtc tattccggta aacttgatgc cggctcctat gtactcaata cccgtaccgg
     3481 taagaaagaa cgtatctccc gcatcctcca gatgcatgcc aacaagcaaa atccgatcga
     3541 attcatcgaa gcaggtgata tcggagctgc cgtaggtttc aaggatatcc gtaccggtga
     3601 taccctttgc gtggaaagcc atccgatctc tctggagagc atggagttcc cggaaccggt
     3661 gatcggtatc gctattgagc cgaaggctca ggcagacgtg gacaaactgg gtatcgccct
     3721 gtccaaactg gctgaagaag atcctacgtt caaggtgatg ttcgacgaag agaccggtca
     3781 gaccgtagtt cgcggtatgg gtgagctcca cctggagatc atcgtagatc gtctgaagcg
     3841 tgaattcaaa cttgatgtga atcagggtgc tccggaagtt gcttataagg aagcgctgac
     3901 caagaccgtg aagcatcgtg agacctataa gaagcagacc ggtggtcgcg gtaagttcgc
     3961 cgatattcaa tttgagatcg gacctgccga cgacgaagtg gttggcctgc agttcgtgaa
     4021 tgacatcttt ggtggatcca ttccgcgaga attcatcccg gctgtagaga aaggcttcaa
     4081 agaagctatg ggtaccggtg tcctggcagg atataccatg gacagcatga aggtgcgtct
     4141 gtttgacgga tccttccacg cggttgactc cgacgccctc tctttcgaga tggctgccaa
     4201 gatcggttac cgtaccgctg cccgtatggc cggcgcccag ctgctcgagc ccatcatgaa
     4261 actggaagtg atcacacccg aagaacatat gggtgatgtg atcggcgacc tcaaccgccg
     4321 tcgtggtatg gtggaaggct ttgacagcaa gaacaacgct accgttatca aggctaaagt
     4381 gcctctgagt gaaatgttcg gctacgtgac ccagttgcgt accatcacct ccggtcgtgc
     4441 taccagcatc ctggagttct cacacttcgc tccgacaccg accaacattg ccgaagaagt
     4501 ggtagcaaaa tcaaaaggta aagtagcagc agaataataa acagacaggt atgagctaca
     4561 agatcagaat gaaactgaag tctttcgacc acacactggt tgacaagaca gctgagcgga
     4621 tcatcaagac cgttcgcagc accggtgccg tagtgaccgg tccgatcccg cttcctacca
     4681 ggaagaagat ctacaccgtg ctgcgttcgc cgcacgtaaa caagaaagcc agagagcagt
     4741 tcgaactgtc aacgtataag cgtttgctgg atatctacag tgcgtccaac aagactgttg
     4801 atgcactgca gaaactggaa ttgcccagtg gtgtagaagt ggaaatcaaa atctaacaga
     4861 acaggcgatg aaaggaataa tcggaaaaaa ggtgggtatg acccactact ttgacaacga
     4921 cggcaggtct gttgcctgta ctgtgatcga agccggcccc tgtgtggtgc tgcaaaagaa
     4981 gaccattgaa accgatggat acgatgctgt tcagataggg ttcgatgaga agaaggaaaa
     5041 gaacacctcg gccggtatga agggtcactt caagaaggca ggtgctgaac ccgtaaagca
     5101 gatcagcgaa ttccgcgatt tcaatacaga actgaatgta ggtgagcacc tcacggttga
     5161 catgttcact gaaggtgaaa aggtacacgt gaagggaacc agcaagggaa aaggttttca
     5221 gggtgtggtt aagcgccata agttcagcgg ggtgggtatg cgtacccacg gtcagcatga
     5281 ccgccaccgt gctccgggtt ctattggcgg ctcttcctgg ccaagcaggg tattcaaagg
     5341 aatgcgcatg gctggccgca tgggtaatga aactgtaaag acccgcaaca tcaagattgc
     5401 caagatcatt tcagaacaga atctgatact cctgaacggc tccgttccgg gaagcaaagg
     5461 ttcttatgta ataattgaga aataactatg aaactggacg tagtcaacag cgccggacag
     5521 aagaccggaa ggcaggtgga gctcagcgat gccatctttg gcatcgagcc gaatgaccat
     5581 tccatctacc tgtctgtgaa gcagtacctg gccaatcagc gtcagggtac ccacaaaagc
     5641 aaagagcgct cagaagtagc aggctccacc aggaagctac accgtcagaa aggtaccggt
     5701 ggtgcccgta agggtgatac caagaaccct atcttctatg gcggtggtac catctttggc
     5761 cccaagccca ggaactacag tttcaaactg aaccgcaagg taaaggaact ggcccgtcgt
     5821 tcagcactga gctacaaggc taagaacaat gagatcattg tgatcgaaac tccggaaatg
     5881 gacaagccat ccaccagaac ctatgcaggt atgctgaaag gtatcggtgc agacggtggt
     5941 cgttctctgg tagtgctcag cgaaagcaat gtgaatgtag tgatgagtgg tcgtaacatt
     6001 cagcgcaccg aagtggctac tgcagcagat ttgaatacat acgctatcct gaaagccaag
     6061 cacctcgtgc tcactgagca gagtgtggcg gcgatcaacc agatggcaga gcaataaata
     6121 aagagagatg tcaagcaaaa gagttttgat caaaccactg atcacggaaa agctgaccca
     6181 actttccgaa ggcctgagaa cttacggttt ccaggttgac ccgaaagcca acaagattga
     6241 gatcaaacag gcgatcgaga aaatgtacgg cgttcaggtg gaagaggtaa gaaccatgaa
     6301 tagcattggt aattacgggt ggcagcgcac gcccttcggc atcagcaaag gcagtaaagg
     6361 ccagatgaag aaggcgtatg taacactcaa ggaaggagaa gaaatagatt ttttcagtaa
     6421 tgtttaagtg acaagatggc tgtaaaaaaa tacaatccgg taacaccggg taccaggttc
     6481 agggtaggta acgcctatgc cgagatcacg accaacgtac ccgagaaaag cttgctggcc
     6541 cccgctaaaa gtacgggtgg acggaacagt tcaggtcata tgaccgtgcg ctatcgcggc
     6601 ggtgggcata agcagaagta ccgtatcatc gactttaaac gcagtaagca tgatatccct
     6661 gcaacagtgg ctaccattga gtacgacccc aaccgcagtg ccttcatcgc cctgctgaac
     6721 tataaggatg gtgagaagcg gtatatgctg gctccgaatg gtctggccgt aggtcaggaa
     6781 gttttgtcgg gcgacagtgt tgcacctgaa gtaggaaatg cactgatgct gaagaacatg
     6841 cctttgggta ccatcatcca caacatcgag atgtatcccg gtaaaggtgg tatgatcgcc
     6901 cgcagtgcag gtacctgggc acagcttaca gccaaagaag ataaatatgc agttgtgcgt
     6961 atgccttccg gagagacccg taagatcctg ctggcgtgct atgcaacagt tggtacagta
     7021 tccaatcctg atcacaacct gcagagcctg ggtaaagccg gacgcaagcg ttggctgggt
     7081 cgcaggccac ataaccgtgg tgtggtcatg aaccctgttg atcacccgat gggtggtggt
     7141 gaaggacgtt ccagcggcgg acatcctaga tcacgtaccg gtctgtatgc gaaaggcaag
     7201 aagacccgtc atccgaagaa gaacagcagc aagttcatca tcagtaagcg taaaaagtaa
     7261 ttgagctatg tcaagatcca ttaaaaaagg accctatata gaccacaacc tcgcaggtaa
     7321 ggtcgagtca atgaatgcta gcggaaagcg cacggtcatt aagacctgga gcaggcggtc
     7381 aaccatttct cctgatttcg tgggccacac ctttgccgtg cataacggta acaaattcat
     7441 cccggtgtac gtgaccgaga acatggtggg ccacaaactg ggagaatttt caccaacccg
     7501 caactttaaa ggacactctg gtaacagata ataagaagaa acatggaagc tgttgcaaaa
     7561 ctgatcaaca acccccgtac caataacaac gcaggctcac cgcgtaaggc acgtctcgtg
     7621 atcgattcga tccgggggct ggatatcgac aaagcgctga acattctgcg cttcaccaat
     7681 aaatcggctg ctgctcctgt agagcaggtg ctgcgtagcg ccatcaacaa ctgggaacag
     7741 aagtatggaa tggatgctgc agagaataac ctgtatgtta aaagctgctt catagatggt
     7801 ggcaaaacca tgcgtcgtta tctccccgca cctcatggtc gtgcatatcg tatgcgtaag
     7861 cgcaccaacc atatcaccat tgtggtagct tccaggaatg aacaacaaaa cgacagtaac
     7921 gaaaaataat atttgaatgg gacagaaagc caatccgata ggcaacaggt taggtatcat
     7981 ccgcggatgg gactccaact ggttcggtgg aaagaacggt gcggagaaac tcattgaaga
     8041 tgagaagatc cgcaagtatc tggatgcccg tatctccaaa ggcggcattg cacgcgtggt
     8101 gatagagcgc accctgaaca ggatcaccat caccatacat acttcccgcc ccggtatcat
     8161 catcggtaaa ggtggtggtg aagtggacaa ggtgaaggaa gagctgaaga agatcaccca
     8221 gaaggaagtg cagatcaata tcgttgagat ccgccgccct gaactggatg ctaatatcgt
     8281 ggctgaaacc attgccaaac aactggaggc caggatcaac ttccgccgtg ctatcaaaat
     8341 ggctgttgcc tcaaccatga gaatgggtgc agaaggtgtg aagatcaggg tatccggtcg
     8401 cctcaacgga gcagagatgg cccgttcaga agaatacaag caaggccgtg tgccgctgca
     8461 tacattccgt gctgatattg attatgcctg gaaagaagca ctgaccgtgt atggcaagat
     8521 cggtatcaag gtatggatct gtaaaggaga agtatttggt aaacgcgacc tgtcgcccaa
     8581 tgtaggcacc cagcaggaac gtcagcgcaa gcgtccgggt cgtggtatgg ctaaacgtgg
     8641 ataataaaag agcagaacaa tgttacagcc aaaaagagta aagcatcgcc gcgtccataa
     8701 aggccgcatg aaaggcaacg caggccgtgg aaaccaattg agccagggaa gtttcggatt
     8761 gaagacactg gaagaagcct ggatcaccga ccgccagatc gaggcagcca gggtagcact
     8821 gacacgtcac ctgaaaaggg aaggtcaggt ctggatcaat atttttcccg acaaacctgt
     8881 caccaagaag cctgcagaag tgcggatggg taagggtaaa ggtgcacccg aatactgggc
     8941 ggctgtggta aaacccggtc gtatcatgtt cgaagtggat ggtgtaccga tggagctggc
     9001 actggaggct atgcgtcttg cagcccagaa gttacctgtt gccactaaaa cagttatcag
     9061 aagagattat accgaataaa acggcagata atggctaaga taaaatcaga agatctgaaa
     9121 tccctgaatg ttcaggacct gcagcaaagg ctggaggatg atcgtgagcg cctgcagaag
     9181 ctgcagttca accatgcggt gaacccgctg gagaatccga tgcaggtaag attcctgcgt
     9241 cgtgagatcg ccaggctgca aaccgagatc agaaaaagag aactggctga aaattaatac
     9301 agatggaaga gactagaaat ctgagaagga ccatccaggg tgtggtcacc agcaataaga
     9361 tggagaaagg aatcaccgtg accgtagagc gcaaggtgaa gcacccgaag tatggcaagt
     9421 tcgttaagaa atccaagaag taccacgctc atgacgagag caatgacgcc aatgtgggtg
     9481 atgtggtcag gatcatggaa acccgccctc tgagtaagct caagcggtgg agattagttg
     9541 acatcataga aagagcgaaa taagatgata cagcaggaat ccaggctgaa agtagccgac
     9601 aatagcggcg cccgtgaggt gctttgtatc agggtgctgg gtagtaccgg cagacgctac
     9661 gcctctctgg gcgataagat catcgtgacc gtgaaggatg ccctgcccgg tggcggtatg
     9721 aaaaaaggaa ctgtttccac tgcagttgta gtgcgcacca ataaagaagt gcgtcgcaaa
     9781 gatggaagct atatccgctt cgatgataac gcctgtgtgc tgctcaacgc ttcagatgag
     9841 ccacgcggta ccaggatctt tggcccggta gcacgtgaac ttcgcgacaa gcagttcatg
     9901 aagattgttt cattagcacc tgaagtgctg taagttatag aacatgagca gattcaagcc
     9961 caaatatcac gtcaagaagg acgatctggt cgaagtcatc gcaggcgaag acaaaggcaa
    10021 gaaaggcaag gtgatcgaaa tcattgctgg taaggaccgt gtgctggtag aaggtatcaa
    10081 tatcatctcc aagcatacac gcccaacagc ccagtatccc aatggcggta tcatcaagaa
    10141 agaagccccc attcacatca gcaatgtatt gctggtcaac cctgcaacag gcaagggtgg
    10201 ccgcaccagc gtgaaagtgg agaacggcaa gaaagaaaga attttcaaaa cagctaagaa
    10261 tagtaagtaa tgagctacgt acccaggctg aaaaacaagt accttgacga agtggtaccc
    10321 caactcaaag agaagttcgg gtataagaac gtcatgcagg ttcccaagct ggtgaagatc
    10381 agtgtgaacc agggagtagg tgccgcaacc caggataaga aactggtgga tgtggcggtt
    10441 gatgagatgt cacgcatcac cggtcaacgt gctgtgccca ccatgtccaa gaaggcaatc
    10501 tccaacttca aactgcggga agagatgccc atcggtgcca gggtgaccct gcgtggtgag
    10561 cgcatgtatg aattcctcga caggctgata gcagtggctt tgcctcgtgt tcgcgacttc
    10621 aaaggcgtta acaacaagag ctttgatggc cgcggaaatt ataccttggg cattactgaa
    10681 catatcatct ttccggagat cgacattgac aagatcaata aggtgaccgg tatggatatc
    10741 actttcgtga ccaccgccga gactgatgcg gaggccttcg aactgctgaa aggactggga
    10801 atccccttca aaaaataaga catggcaaag aaatccatta tagcaagaaa cgataaaagg
    10861 gaaaggctgg tagcgctgta tgcagagcgt cgcgagcaga tgaaaaatga cggtcgctgg
    10921 gaagacctcg ataagcttcc cagaaactcc agcaaggtgc gcctgcgcaa ccgctgtcag
    10981 ttgaccggtc gcccccgcgg atatgtacgt gatttcggta tttgccggaa ccagttaagg
    11041 gaaatggccc tgtacggcaa gatcccgggt atgaaaaaag caagttggta aaaaacagaa
    11101 cgatcaacag atgaaaacta ctgatccgat agcagacttc ctgacccgca ttcgcaatgc
    11161 gcaacaggcc aaccacagag tcgtggagat acccgcttcc aatctgaaga agcgcatgac
    11221 cgagatcctg tatgagaacg gctttatcta tcgctataag ttcgatgata atggccctca
    11281 gggaaccata aagatcgccc tgaaatatga tccggtatct aaggttcctg ccattcgcgg
    11341 actggaaaga gtgagccgtc cggggctgcg ccattatcag catgtagatg gtttaccccg
    11401 tgtactgagt ggtctgggta tggctatcgt gtcaacttcc aaaggagtga tgacagaaaa
    11461 agaagcccgc cgccagaatg tgggcggaga ggtactctgt tatgtttatt aatcagcgag
    11521 aaaagaaaga atcatgtcaa ggataggaaa agcacccatt gcactccctg aaggagttac
    11581 ggtgaatgtc agcgacaaga acctcgtgac cgtaaaagga aagcaaggag aactgaccca
    11641 gcaggttgac cgcgatatca tcgtgaaagt agatggcaat caactggtag tggaacgccc
    11701 cactgatcag aagcggcatc gtgctatgca tggtctgtac agagcactta ttgccaatat
    11761 ggtgaaaggt gtttcagaag gtttcaaaaa gcaactggag ctggtgggtg taggttatcg
    11821 tgccagccat cagggtcaga tcctcgaact ctctgtaggc tattcacacc ctatcatggt
    11881 ggtgctgcca aaagagatca agctgaacac catcagtgaa aaaggtaaga acccgactgt
    11941 tgtactggaa agttcagatc gtcagttgct cggtcaggtg tctgccaaga tcagaagcct
    12001 ccgcaagccg gaaccatata aaggaaaagg tattcgttac agcgatgaag tactgcgccg
    12061 caaggcaggt aaaaccgctg gcaaataaat agcagagcta tgcagaacaa aaaagcccag
    12121 agaagaagaa agatcagata cggcatccgc aaaaagatcg cgggtactgc tgacaggcct
    12181 cgtttggcgg tatttcgcag caacaaacag atctatgccc agatcattga cgatgtggca
    12241 gggcatacgc tggtggctgc ttccagccgt gagctgactg atgccaaagg caacaagact
    12301 gagatcgcca aaatggtggg aacggaactg gccaagaaag catctgaaaa agggatcgca
    12361 tctgtagcat ttgaccgcgg ggggttcctg tatcatggaa ggatcaaagc cctggccgac
    12421 ggtgcacgtg aaggaggatt aacattctaa ggaaatagta tggcaacgaa taaagagata
    12481 caaagggtta gacctaacga gaccgaactg accgaaaaga tcgtcggtat tcagcgtgtg
    12541 gccaaggtga ccaaaggtgg ccggaccttc agcttctctg ctcttgtagt agtaggcgat
    12601 ggtcagggta cagtaggaca cggactcgga aaagcacgcg aagtgaccga agcaattcag
    12661 aagggtattg atgatgccaa gaagaatctg atcaaggtac cggtacataa aggcaccata
    12721 cctcacgaac aaaccgggaa atacggtgct ggtaaggtca tgctgaaacc cgccgcacat
    12781 ggtacaggtg taattgccgg tggtgccatg cgtgcagtac tggaaagtgc aggtgttact
    12841 gacgtactgg ctaaatcaca agggtcttcc aacccgcata acgtggtgaa ggctactctg
    12901 gatgccctga gcaaactgcg tacgccaatg gctgtagctg cccagcgcaa cgtttcatta
    12961 caaaaagtat tcaagggctg atatcatgga aaaactgaag ctgaccgcag tgaaaagcac
    13021 tatcgaccgc ccggccaaac aaaaggccac cgtaaaggcg ctcggcctgc gtaaactgaa
    13081 ccagaccgtc gaagtggaag ccaccccgca ggttctggga atgatcgaga aagtgaagca
    13141 tttactgaaa gtagaaaaag cataaagatg gatttgtcta atctgaaacc tgccaagggt
    13201 tccgtcagga agaacaagcg tattggtcgc ggtcagggct ccggtcacgg tggaacttcc
    13261 acacgtggtc acaaaggtgc caacagccgt tccggtaaca agaagaaaca ccactttgaa
    13321 ggtggtcaga tgccattgca gcgtcgtgtg ccgaaacgcg gtttcaagaa cccgaatacg
    13381 gtggaataca ctaccctgaa cctggatcag atccaggaaa tggcagacaa atacggtatg
    13441 accggttttg atcaggagat cctgatggcc aacggcgtca tcggtcgcaa tgataaactg
    13501 aaagtactgg gccgtggcga gctgaaagca aaagtggaca ttcatgcaca tgccttcagc
    13561 aaaacagcca aggcagcaat tgaagcatta ggtggaaccg caaacgtgat cggttaacag
    13621 atgaaaagac tgatagagac attaaagaat atctggcata tcgaagacct tcgaaagagg
    13681 atcctgtatt ccatcggact gattttcatc ttccgtctgg gatcttatgt gatactgccc
    13741 ggtattgacg ctgacctgta caaacaggca gtggaaggag gcgccagtgg cggcctgctg
    13801 ggattgatca acctctttgc aggtggcgga ttcagccgtg catcggtgtt cgctctgggt
    13861 atcatgccct atatctcagc ctctatcgcc atgcagctgc ttaccatcgc agtaccttct
    13921 tttgcccgtt tacagaaaga aggcgaaagc ggccgccgta agatcaatca gtacacacgc
    13981 tggctgaccg tagtggtcac agctttccag gcatctggtt actatatcta cctgaagtct
    14041 ttgtcagaag gaaatggtgg tatagacttc atcgtgacca gcaatggtat gttctggttc
    14101 atatgtatga tcatcctgac caccggtact ctgttcacta tgtggctggg tgaaaagatc
    14161 actgaccgcg gcgttggaaa cggtatctct ttcctgatca tggtgggcat catcgcatct
    14221 ttgcccgcgt cactgatcaa tgaatttacc agccgtgtca atgagaatgc aggcggtctg
    14281 gtacctttcg ttctggaact cggcttcctg gtagtggtgg tcatgggttg tatcctgctg
    14341 gtgcagggaa cccgcaggat ccctgtccag tatgccaagc gggtggttgg tacccgtcag
    14401 tacggtggta accgtcagtt cattccgctg aagatcaatg ctgcaggtgt catgcctatc
    14461 atctttgccc aggcactgat gttcattccg ggcactgtac agcaattctt cccggattca
    14521 cagagtaatt tcattatgca actcaatgat ttcacctctg tgacctataa catcatctat
    14581 ggatttctgg tgatcatttt cacctatttc tatactgcca tcaccatcaa cccgacccag
    14641 atcgctgatg atctgaagaa gaatggtgga tttgttcccg gtgtgaagcc tggcaaaaag
    14701 acggcagagt ttattgataa cgttttgtcc cgtatcacac tgcccggaag tatcttcctg
    14761 gcgctggtgg ccatcatgcc ggtgttcacc acagcctttg gtgtgggcag ccagttcgcc
    14821 agattctttg gcggtacatc gctgctcatc atggtgggtg tgatcctgga tacattgcaa
    14881 caaattgaaa gccatctgct gatgcgtcac tatgatggtt tgatgaaatc aggcaggatc
    14941 aagggtcgct cttcaggtgc ccagactcct gtaggaatgt aaggatgatc tattataaga
    15001 catcagatga ggtcgaactc atcagaaaga gttctttgtt agttggaaaa acgctggctg
    15061 ccgtggcagc tgcaatcaaa cccgggatga ccaccctgga attggataca ctggccgaaa
    15121 cagtgatccg cgaccatggt gcagttccgt ccttcaaagg atacaacggg tttccgggta
    15181 cactttgcgt gtcagtaaat gagcaggtgg tacatggaat accgggcaac cgggtcatcc
    15241 aggatggcga tgtagtgtct gttgattgcg gcgcattcat gaacggcttt catggcgact
    15301 ctgcctacag tttcctggtg ggaaatgtac cggcagcagc ggtcaggctg gcgaaggtta
    15361 ccaaagagtc tctgtatctc ggtatcggga aggcagtggc tggtaaccgc atcggcgata
    15421 tcagtcatgc cattcagtcc tattgtgaaa cacaattggg ttatggtgtg gtgagagagc
    15481 tggtggggca cggcatcggc agagaactgc atgaagcccc cgaagtgccc aatttcggta
    15541 cccgcggcag gggagtgcag atgcaggaag gtctggtcat agccattgag cccatgatca
    15601 accttggtgg acgccaggtg gtacaggaaa aggatgggtg gaccatccgc acccgcgaca
    15661 acaaagtgtc catgcattat gagcacactg ttgcagtggg aaaagaaaag gcagatatcc
    15721 tgtcttcctt cgaagagatc gaggaagcag tgtcgaagaa tgaatattta ataacaacgg
    15781 aagaggttac ggaacagtaa tgtcgaaaca aggagtcatc aagcaggacg gtgttgtcac
    15841 ggaagcattg ggtaatgcaa agttccgtgt caagctggag aacgagcacg agatcctggc
    15901 caccatttcc gggaagatgc gcatgcacta catacgcata ttgccgggcg acagggtgac
    15961 cgtcgaaatg tcgccgtatg accttaccag aggaagaatt atttacaggt ataaataata
    16021 agtaatcatg aaggttagac cgtccatcaa gaaaaggagt gccgactgca agatcgttcg
    16081 cagaaaggga cgcctgtatg tgatcaacaa aaagaatccg aaattcaaac aacgtcaagg
    16141 ttaaaataaa gtaatatggc aaggatcgca ggtattgacc tacctaagaa caaacgcgga
    16201 gtcattgcac tcacttacat ctacggcatt ggcgacacca ctgctgccaa gattctgacc
    16261 actgccgggg tggacctgag caagaaggtc aaagactggg atgatgatga agtgaatgcc
    16321 atccgcaaca ccatcaacaa cgagtacaag gtggaaggtg cccttcgttc agaagtgcag
    16381 ttgtccatca agcgactgat ggatatcgga tcttaccgcg gtgttcggca tcgtaaagga
    16441 ctgcccgttc gcggtcagca aacccagagc aatgcccgaa ccagaaaggg aaggaagaag
    16501 acagtggctg gtaagaagaa ggcacctaaa ggataaaagc agttgaatct tgaatcataa
    16561 cagtaatggc taagtcaagt aaaaaaacaa ccaagaaacg agtcgtcaag gtagacgccg
    16621 atggtcaggc ccatatacag gcctctttca ataacctgat cgtaacgttc acgaacggaa
    16681 gcggtcaaac catctcctgg gcttctgccg gtaaggccgg ttttcgcgga tcaaagaaga
    16741 acacaccgta tgctgcacag gttgctgtca gcgatgctgc caaggtagca tatgatgcag
    16801 gcctgcgccg tgtggatgtg tttgttaagg gtccgggcag cggacgtgag gcagctatcc
    16861 gtcagatcgc caactccggt atcgatgtga tggtcataaa agacatcaca ccacttcccc
    16921 acaacggttg tcgtccgccc aaacgcagaa gggtgtaatt attaagcata aagaaccaga
    16981 agtaacatgg caagatatac aggacccact tcgaagaaag cgagatcatt cggcgagccg
    17041 atcttcggat ttgataaagc ctttgaaaag aggaaatatc ctcccggaca acacggagtt
    17101 tccaagcgcc gtaagtcgcc gtcagaatat ggcgtgcagc tgaaagaaaa gcagaaggcc
    17161 aagtacactt atggtgtgct ggagcgccag tttgccaaga cctacgagga agctaccaaa
    17221 cgccacggag ttaccggcga ggtgctgctg caattgctgg aggcccggtt ggacaatacc
    17281 atctaccgta tgggtctggc tccaaccaga agagcagccc gtcagatggt gaaccacaag
    17341 catatcgaag tgaacggaat ggtgtccagt acaccctctc acagccttcg tccgggcgat
    17401 gtgatcactg ttcgcgaacg ttcaaaagat atgaagatca ttcgcgaagc aaatccggat
    17461 tcacgcaagt acaactggct ggagtggaat ccagaacaac aaagaggcgt tttcctgcag
    17521 taccctgaac gggatcagat acctgaaacc atcaaggaac aactgatcgt ggaattgtac
    17581 tctaagtaat tcatcaacct aatacaccag aaattatgtc catactcact tttcaacgtc
    17641 ctgataagat cactctgcag aaatctacag acttcgaagg gacctttgaa ttcaagcctc
    17701 ttgaacccgg tttcggtgtt accgttggca acgcactcag aagagtactg ctgtcatctc
    17761 tggaaggctt tgccatcacc tctgtcaaga tcagcggtgt ggatcatgaa ttctccacta
    17821 tcagcggcgt catggaagat gtcactgaga tcatcctgaa cctgaagcag gtgcgcctga
    17881 aaagggtgat gggcagcgat gagaaccagg tagagaagat cttcatttct cttaaagaca
    17941 aagaagagtt caaagccggc gatattgaga agcataccaa tggctttact gtcatgaacc
    18001 ctgacctgct catttgccgc atggaatctg tggtaaaact ggacgtggaa ctgaccattc
    18061 agaaaggtcg tggttatgta cctgcagaag agaacaacct gctggaacaa accatcggtg
    18121 ttattccgat cgatgccatc ttcaccccga tcaaaaatgt gcgctatacc atcgagaaca
    18181 ctcgtgtaga acagaaaacc gactacgaga agctgctcat tgagatcaaa acagatggta
    18241 ccatccatcc ggaagatgct atcaaggaag ctgcccgtat tctcattcag cacctgatgc
    18301 tgatcactga tgagaatatt accttccata caagtgtaga aaaagaagtg gatgccgttg
    18361 atgagcatac cctgcacatc cgcaagctgc tgaaaacacc actggaagat cttgatctct
    18421 ctgttcgggc ttataactgc ctgaaagctg ccaagatcaa caacctgtca gaactggtaa
    18481 aatatgacac caacgagctg ctgaagttcc gcaacttcgg taagaaatct ctcgtagaga
    18541 tcgaagctat ggtcatagac aagggcctgc agtttggcat ggacctgagc aagttcaaac
    18601 tggaagaaga ctgatcctta aagaagaaaa tatctgatca atgagacacg gaaataagat
    18661 caatcacctg ggtcgtacct tcagtcatcg caaggcgatg ctgagcaata tggctacgtc
    18721 gctgatcatg cacaagcgta tccataccac ggtggccaag gctaaggagc tgcgcaagtt
    18781 tgtagagcct attatcaacc gctctaagga agataccacc aacaaccgcc gcgaagcatt
    18841 tgctgcactg cagagcaaag aagccgtgag cgaactgttc aatcacattg gaggaaaagt
    18901 ggccaaccgc aatggtggtt atacccgcat cctgaaagtg agcgacaatc gtgccggcga
    18961 taatgctgag atgtgctata tcgagctggt agattacaac gaagctatgc tgaaagaagc
    19021 aaaaggccct gctaaaaagc gcactcgtcg cggtaccgca aagactgcag cacctaaggc
    19081 tgagaagaag gaagaagtga ctgcagctgc tgaagaagct gtggaagaaa tcaaacctga
    19141 ggcagaagat gccgcagctg atagcgaaga gcaggcttaa tcagcttgta actaacatac
    19201 gaatggccac cccggggtgg ccatttcttt ttctgtgtgg tttgctgatc actgattcac
    19261 ataaactttc ctgctcagcg ttccggaagc tgtttccagt ctgactacgt atattcctgc
    19321 aggcaattga ttggccatga ccgttgtatg tgttccgtcc atgttgcctg ataataaggt
    19381 tcgacctgac agatcaagca cctggtaagt gcctgtttca gcagtgctgt tttgaatgag
    19441 atgtatcatc gaaccatagc tgtacaccgt ccagttggta agtaccgcat cctgaattcc
    19501 ggtggatatg acagtgatgc cctgacctgg ggtggcctca taagcataca tactcactgt
    19561 aaatgattta ttgtcactgc caacatccag tcgcagccag ccatagtggt cggctccgtc
    19621 cttgtgcagt ttcagcccga tgaatttgcc gtctgtaccc cccagccagt ttccgttttg
    19681 ttctaccggc gacagacctc cgccactgga atattgaaag ctgtaggcca gtgactgaaa
    19741 ggaaactgcg gcaaacagat cagatgaccc gatcatatcc atactgttca ggacagccgg
    19801 ataagcaaaa ccactgaata cgctgttgac cagtatttca ttactgccat agggtgaagc
    19861 aaacacaccg ttaatggcat actggttggt tccgccactg ccaattacaa acgctgcagc
    19921 tgaagtggct gaaaaaagaa agtcttctgt gccgtcgttg tccaggtcca gatagaagac
    19981 gccgtcgggt gcactataag tgacatccgg gtctatatct gtaagcacaa cctgtgcttc
    20041 agcacctcct gctgcaagga aggtaagagc tgccgcagaa taggcttttt ttctggaaat
    20101 tcgacgaaga gtggtcttgg taactgtcat aaaagagggt ttcaaaatgc agcactaaga
    20161 taaagtttcc gctgcaggat caggtcgtta aattgtatca tgatattgta aatacctgca
    20221 ggcaggtctg tgtcaatctg caatagtggc tgatcaatcc ggcctgacaa tacttcccgg
    20281 ccggtcaggt caaccaggcg gaaaggggca ccggtcacgt cattgcctgt atgaatgacc
    20341 aggcggttat tccaggtata ggcattccat cctgtgggcc acacaccggt aatagcatca
    20401 ggagcgggat ggccagtctg gatacaggaa tcaggcacag cagaaatagc gtattccttc
    20461 acggtgaaag caatattgtc tggcgcaaca tcaaggcgca gccacccgaa ataatgttcg
    20521 ccatcctgac gaagctgtaa gccgaggtat ttgtctgttt gtccgccaaa ccagaaacca
    20581 tcgccgttca gctgtacata agagaccaca gaacttccca gattcagttt gtacccatag
    20641 ctgtacacga ggctctgata tccgccttcc atccattcgc cgtcttcaca cagccacgca
    20701 agactgtcca gtgcaaaggg atactgaaaa ccgaaagtgg tgatatctcc caggatggca
    20761 ttgcttccca gggcagtgcc atatatgtgg ttcagttgta tggtaccact atacacattg
    20821 ccataggtag tatatatggt tgggttgaat gaagacagaa atcttttggt tagacggaag
    20881 tccggagtgg catcaccatt caggtcaagt tcaaaaaaat cgccgttcag gtcaatggtc
    20941 acatccgggt caatatcggt gtagatgacc tgaccttcag cctcacttcc aacagctaca
    21001 aaggttgctg ccagggcaga ataaccgagc agggagggcg tgtttttctt tttcatacgg
    21061 ggtattgaaa tatcacaaca aaggtaaggt atataatggc aatgaaccgg tcatatgaca
    21121 ggagagatgt ttctaatatg cggggcgttc gatcattcaa taaagacctt ccgggtcaac
    21181 cggaaaccgg ctgtctctac ctgtaccaga tacacaccgg cagctgcctg atccagatgg
    21241 atctgcatgc cggaaggaac caggtttcca ctgaacacca cctggccctg tatgccggtt
    21301 atatgaactg tggcttctgc agcaggtgcc tctggtgttt gaatgacaac ggtctggccg
    21361 aagctgtaga tctctgtctg accgatggcg gaatattcca ggaccggaac caactcattc
    21421 tgacctgttg ctattgccat atcaggaatg gtattatatg cataatcttt tatggtacag
    21481 gtggtgtggt cagctgctac atccagtctg acccagccgt aatgcgtctc ttcacctatc
    21541 atgaactgca gcccaaggta tttgtctgtc ttgccgccca gccagtttcc gcgttgcaca
    21601 ttagagacag acaagccgga tgcttccacg ctgtagtatc ggctgtacac aaggctttgc
    21661 aggcctgcag gaatcatctg acttgcattg ctgaccgact ggcccgcatt cagggcaaac
    21721 ggatacggat agccgctgta gccataggag cctgacatgg cattaccggt tgcgggcagc
    21781 gcaaagaggt ggttaaaaag tatggttccg cttaccgagc tgcctgatgc ggtactgaaa
    21841 atgtagggat aagctgaaaa atatttgtat atgacaaagt ctgttgttcc atcggcgtcc
    21901 aggtccaggt taaacagatc accttgcatc tccagggtaa tatcaggatc tacatcagtg
    21961 tactggatca cgttctggcc gaaggtactt ccggcaccca gcagggttgc tccggccatt
    22021 gcagaatagg ctgcaagctt tgtagaaaag gcactttcat tattcataca gtacaaaatt
    22081 actgtatgtg gtgtcaatgg cctgcagacc cttgtcagtt ctggaacaga tttgtctctg
    22141 caggaggtca ggtgcaaaaa aaagcccgcc aatggcgggc tttgtatagg atcaatgtac
    22201 aggtcactga ccgatgaaca cttctttgct gaatacggca ccgtcagcaa cgatacggat
    22261 gctgtacaca ccggtagcgg cgttgttcag cacgatatcc atacctgaca tgtccagatt
    22321 gttgttataa acaacctgac caagtgcatt ggacacctgt acctgtgcat tgtttgcttc
    22381 cagtccgttc acgttaatgt gaatggtatt gccaaagctg taagcagtaa gctgagaagc
    22441 atcgatcagc gacagttgaa caacatcgcc catgtcgcct gtttcgatag actcaccagc
    22501 agtgctgttg taagccaggt ctttgatggt cagggtaaca ggacctacag ttccgtccag
    22561 acgcatccag ccgtaatggg tgttgccatc cacatcaaat gaaaagccga ggtacttgtc
    22621 agtaacatca gcaaactgac cgtaagtagc accggagtag atagagttca ggaacgcaac
    22681 gttgccgggg ttgctggagc tgtcgcccag gaaatctgca ctggcactta ccagagcacc
    22741 tgcatccaga gcgctgccgt aaggcaggat tgcaccactg taacccatta ctttgttgct
    22801 tactgcacct actgagtaag aaggtacgtt accgaacaca cgaccaaatg accatacacc
    22861 accggtagaa gaagtagcct ggaacaggaa gtcatcataa ccatcgccgt tcacatcaac
    22921 cagaacgata tcaccaattt caatggtcac atcgtcaatg tcttcataaa caatggtctg
    22981 tgcatctgcc tgagtagcta ccatagtggc agccatggct gaataggcag ccagtttctt
    23041 gctggcagat tcgttgtaaa tttttttcat acgtaatcga tttgtaagat ttttaccccg
    23101 ctaatgtacg caggattttt gttttgtaaa ggataaatta accaaaacag gtcataatcg
    23161 tgctaaaatc cggacgccag aaatattttc ctggttgctg taccgtgttc tgctgagctg
    23221 atctgaacca ggtatatgcc atcctgaacc gctgttgcag gtagcaaccg gtctggttga
    23281 cctgcctgct gtgtaaacgg aatgagccgg ccttgcagat cgaacaggga atagtgcacc
    23341 tgccactctt ccggtcgttg taccagcaca gcctgcccgt ctgtccagca ctggatggct
    23401 gatgcaaggg caggttcagg cacaggtgcg gtgatacctg cagtaatgcc tcctatattg
    23461 gcctgatcat cccaggcgta ttcttctatc gtaatggagg tagcggccat accaacagac
    23521 aggcgaatcc aggcataatg gctgtcatct ccaatcttga aacgcatacc cagcatgccc
    23581 gacagaccgt tccacggccc gctgcttaca aagggaatgc catccagctg cacaaaagca
    23641 gccagcctgg ccgggcccgg aaacaactcg agtgtgtcag caatgaaggc acctgatgaa
    23701 aagttgtagg gcatgaggaa ctcataacca ccggaacttc cgggctcacc gaagatcaca
    23761 ttattattca gaccggaagc ataggccatc ctgtaggtgt agctgaaact tcctccttct
    23821 gccgtgctga tcattccctg ctggcttctg atccagaaca tcaggtcatc ggtgccatca
    23881 ccatccagat caagataggc ggtatcgccg gggttgacca ggagatcagg gtcaatattc
    23941 tgataaacca cctctgccga ggcatctgca gaccatagca ggagggacga cagagcagaa
    24001 tatgctgcca gccgtccggt cagttgctga tcattcttta gtttcatttg ctttttctct
    24061 gtataacgaa cgatcaataa cacctgccat tgcagctgtg tccagtttgc cacccagaaa
    24121 ctggttgatg cggctgatct cttcacccgg gttattcatc atggcagcat gagatacata
    24181 tagaatatcc acatttgcct gattggttgc ccaattgcgc accttctcca ggttttgcct
    24241 gaagggtccc atcaatttca ggttgatggt atcttccttg gctttgccgt ttctgaccag
    24301 catgcggtgc tggctcatca gtacctcatt gaggtctcgt tccatgaaca ccactttata
    24361 gttgtagcgt gcaggcagct caaacagaag gtggctgata accttgacca ctttcccttt
    24421 ggcttcttcc agccaggcct tgttcttgga aatggacatg acagcctcat gctcgtaata
    24481 ccctttaggg ttgttttcgt ctgctgttct tttcttgtct gaaaagatct ccagtccgcc
    24541 tttgtcgagc atttgcatca tcatactggt acctgatcgg ggcagacctg ataccactac
    24601 cacatcaccc cgcatatggt cttccatggc ctcggttttc tgctcggcca gttccggttt
    24661 gttcaggtgt tcacggtaga tcctggaaag ccactgtctg gccttgttca caccgggagc
    24721 cattttgagt gttacctcaa aagcttcggc agaacgttcg tattcgccca tatgatacag
    24781 ggtctcaccc aggtgataat gtgcaaacgg aaagtggtag atgaagccca ctgcgttgag
    24841 gaaacagtct gctgcttctt cataattacc ctgcctgagc aggctgacac ctaatccgtg
    24901 ccaggcactt gcatttccgg ggtcatgttc cagttccttg cggaaggctg cttctgcctt
    24961 cttccagttc ttgagcagga gataggattt tccaagttgc agccctaccc ttgcacctac
    25021 atgctctttt tctatggctt caaatatctc cgtggcttct ttgggtttgt tttccatcag
    25081 cagcagaccg ccttccatga cctgcatacc aacagattct gatccggtgg cttctttcag
    25141 gttcttcaga acggtgcggc attcagcgat cttgccggtt gactgatagc aatgcgccag
    25201 gcgcatggcg tatcggttta catcagggtt ttctttgaca agaccttcca gtatctgaac
    25261 ggcctcctca gtttttcgtc cgtgtatgta agcccgggcc aggtagtaat tgttctctgc
    25321 aactgttttc tgaatggctt tctcggcatt ctcatccggt ctttccacat accccagttc
    25381 tatcagttgt tccatggcct catgggctgc ataggggtct tcctgacggt ctgccgggtg
    25441 ttgcccgctg ggccccggaa tgttctccca gctgtcaatg cggtctggag tgatctgttc
    25501 ttcaaaagca ttgatcataa cgttgccatc catatcgttg gctaccggca aaccgaataa
    25561 agtgagcagg gtcggggtaa catccaggat gctggcaccg taaatgcgct catccttttt
    25621 aatattgggc ccgttgatga ccacgatgcc atacggactg tgttccagtg caggagcagc
    25681 aggttctttg ggaagacgca ggggcctaag atgatcggga tggaagccgt ggtctgaaat
    25741 cagcatcacg gtcacatcat cgcctgccag ctccagcagc cgttccagca tcatatcgtg
    25801 gaatttataa ccactgatga ccacgtcttt gtaaagttgg tagaggtcat caggaacacc
    25861 gggcaactgc ggcggatgaa acttcatgaa accatgacag aagtgatcaa tggcatcaaa
    25921 ataaacgcca acaaaatccc attcttcatt ctctaatata taggttgctg ctgcctgtat
    25981 ggaagaggca tcggcaatga cctttgccag catttgcagg cgtttgtctt tttcctggtc
    26041 aacctttgca gcatccggaa caaagggcag cagatgagct gctgaaagtt cgccgggatg
    26101 caccctgaat ttttcaaaga tccctgccag gttttccgga tgtaccgttc ccggagccat
    26161 gggccagggt tcatgtatcg gcttggtggc tttctggaaa aagttggata ccatgatgcc
    26221 attgatgggt tctgcaggat ggcttggcca ccagcccacc acatggcttt tcatatgatg
    26281 ctgattgaaa atattccaga ctgccttcac ctttctggag gttatgaagg ccggccttac
    26341 tcctttgcca ctggggtctg gctcggtgaa accgtgaata ccatgtttat atggtctctt
    26401 tcctgtactg atactggtcc agagcatggg cgacagtggc gggtccagcg tggccaggtt
    26461 ccccattaca ccaccgttca ccagtttttc cagagtgggc atatgcccgg catccatcag
    26521 cggattgatc actttccagt cggcggcatc ccagcctatc agcaatactt ttttagctaa
    26581 tcgcttaccc atcagtcctg tttcttttgt tcctgttctg tttgttgcgg caacttacct
    26641 gctgcctgca gttccttcat cttttcagca taggcagccc ttgactggcg ccaggccatc
    26701 aggcctacat gacccagtgc cagcaagccc agcgaaccct ccggtggaac cgcgaacggc
    26761 ttaccatctt tggtggtgat ggtgatctgt tttacttcct ttttattctc cataatagga
    26821 ctgcaaaggt accaaattcc ggcctgccct gacctaccag gacaactgcc cgtattccat
    26881 tgtatttttg tgggctcaaa cagaaagtat ggcattgagt gaacaggaga tcatcagaag
    26941 agaaagtatg cacgcactcc ggcaaatggg catcgaaccc tatccggcag cagcggtgga
    27001 agtgaatacc aacagccgtg atatactgga tcgttttgat tcagaaccgg aggcatttgc
    27061 cggggtgaaa ctggcaggaa ggctgatgag taaacggatc atgggaaagg cttcttttgc
    27121 ggtcattcat gaccaggaag gccctatcca gatctatatt gcccgcgatg agatctgtcc
    27181 ggaagaaaat aaagacctct ataacgaagt cttcaagaag catatggaca ttggcgatat
    27241 cattggtgtg gccggacatg ttttcaagac taaaaccggt gagaccacgg tgcacgtgac
    27301 agagctgaag atgctcagca aatcactgcg accgctgccc attgtgaaga ccgacagtga
    27361 aggtcaggtg catgatgcct ttaccgatcc ggaacaacgc tacagaatgc gctacctgga
    27421 cctgatcgtc aatccgcatg tgcgggaaac atttgtcaag cgcacaaaga tggtcaatgc
    27481 catgcgtgat tatctgaatg aaaggggata tctggaagta gaaactcctg tccttcagcc
    27541 attgtatggt ggtgcagctg ccaggccctt taaaacacat cacaatacgc tggatatgac
    27601 cctgtacctg cgcattgcca atgagctgta cctcaagcgt ctgatcgtgg gtggttttga
    27661 tggtgtgtat gagttcagta aagacttccg caacgaaggc atgagccgtt ttcacaatcc
    27721 tgaatttacc caggttgaat tatacgtagc atataaagat tacgaatgga tgatggacct
    27781 ggtggaagaa atggttgaac gggtggctat tgccgttacc ggtggaacca ttgttcaggt
    27841 tggtgaacat gccatagact tcaaacgccc gtggacacgc tataccatgt tcggtgctat
    27901 tgagcatttc accggggttg acatcagcgg aatggatgag aaagcacttt atcaggcggc
    27961 gaaagatatg catgtgcatg tagatgcctc tatgggcaag ggcaagatca ttgatgagat
    28021 ctttggtgag aagtgcgagc cgcatctcat acagcccacc ttcatcacag actatccggt
    28081 agagatgagc ccgctggcca agaaacaccg taccaaagaa gggctggtag aacgatttga
    28141 agctgtggtg aacggaaagg agatctgcaa ctcattcagt gaactcaatg accccataga
    28201 tcagcgccac cgctttgaag cacaattgga actaggtaaa cggggtgatg aagaaagcat
    28261 gctgctggac gaagattttc ttcgagccct tgagtttggc atgcccccca ctgccggatt
    28321 gggaatcggg atcgacaggc tggccatgat catgacgaac agccagtcta tccaggatgt
    28381 catcttcttt ccgcaaatgc ggcccgagaa atgacaagac acctgtgagg catgcaggtt
    28441 gaagtgagct gttcagcccc aatggctcag ggctacctcc tgaaaaacaa aagcgggttc
    28501 tgaattttca gaacccgctt ttgtttttca atggcaatta tttgtttttg acaaagcgca
    28561 gggaagtgga ttcaccattg tttcccataa gctgcatgag ataggtgcct gcatccagat
    28621 cgccggtatt cagggtaatg acctgctggc ctgttttcag gctcacatcc tgttgcatga
    28681 tcacctgacc ctgcaggttc atgatataga tggttccttc ctgtgcagca ctctgcacca
    28741 ggcttacctg catcacatca aacaccggat tgggtgaaac actggtatgc gcaaaccctg
    28801 cagatatcct ggctgtgatc acattgctga agtgctgtaa accgttgtag tcattttgaa
    28861 ccagacggta ataatgtgtt ccggcaaaca ggtcttcgtg caggaagctg tactgatgcg
    28921 catcggtggt agtgccgttt ccggcaatgg taccgatggc ttcaaaatga ctgccgtcca
    28981 tgctgtgttc cagcgtaaag aagtcattgt tgatctctgt gccggttacc cagttcagcg
    29041 ttacatcatt ctctgccaca taagcttcaa atgtttcaag ggtgatgggc agtacccact
    29101 cctggatgat cagcagatta tcaaaacgca tctctgaggt cagcgcactg tggctgtaca
    29161 tcgggccggt atagagaaaa cctgaactgc cggtgtaagt gttatctaca ctggatccga
    29221 tggtggagta gcttgcagtg cccgggtcag ggaaactcac atcgctggat gcatactgca
    29281 gggtccagtt gtctgataca ggatcataaa tgaccttgac cgccagatac tcattcagga
    29341 agtcaccacc cttaacaata ttggtcaggt tggttccatt atccagtcca tcgttatact
    29401 ttaccagtcg cagcctgtcc agtgagccgg gatttccaag cactactgca tagccgttgc
    29461 cggactcgaa attctggttg ttggatgcca gcacatatgc cataccattc tgtgaggtac
    29521 ctacaccact cagagaagca gcgtcagact gcatgttgaa ggtccagaca ataggcgctg
    29581 tattatcagt catagtgctg acatagaaag gcgatacaga tgtcaggttg acaaacgccc
    29641 agtcacggtc agtcactcct tcactgggga aaacaagttc atcgcctgat acaaagccac
    29701 gcaatccacc accttcaaac cagctcagtc cgttcatggt tgtacccagt tctgtagtgg
    29761 tgggcggacg agtaaaatca tcttccacga ttatgttgaa ttgcccgtaa gccacagaag
    29821 cggagagggc taccgtcatc agaaggctaa gcacagatct catacctaaa atttgtcact
    29881 aaattacatc aaaaacaggt ttcccaccgg aaatcaacac caacaaagca agaccttcgc
    29941 atcacaaatc agacaagatt ttattgattt tctttaaaat tacagggaaa aaggcatttg
    30001 aaaatatttc atactgtcac aaaatgatat ttcatgttgg tatattttct ttccgggatt
    30061 ccctatcagt cagggcaggc ttagagaaat aaccttgcgt tcatatttcc tatccgcgaa
    30121 gcacatagat ttgtggttta aactcaatta atttatgcgc acatttcaac taaccttact
    30181 ggctgtggca cttggtttct ccggtgcagt ggcagctcag tcgctctctg cgggcgacat
    30241 tgccatcgtg cagatgaatg ccgatggaac agacaactgg gcttttgtgg ccaccaccga
    30301 cattccgggt ggcgaaatga tcagcttcac tgacaatggc tggaaggccg acaatacctt
    30361 ccgcaccggt gaaggtatca tcacctggac cgctcctgaa gaaggggtgg cttgtggtac
    30421 ggtggttacc atcgactaca atgctttggc atcagtggga tcagcctctg aatcaggttc
    30481 tctggacttc agttcttccg gcgatcagct cctggcttac cagggcagta ccgaagctcc
    30541 ggttttcctg gccgcactga ataacgatgg cagcgctgtc tggcaatctg atgccaccag
    30601 ttccaacacc tccgctctgc ccatgggtct gaccaatggt gtgaatgcag gggctgtcaa
    30661 tgaactggat aatgtggcca gcaccattgc ttctatcagt gatacccgtg ccaacatact
    30721 gagtacggtg tatgacccgg ccaactgggc tggtgatgat accaaccccc agacctttgc
    30781 cggaactgtt gaggttactg aatgtgcacc tcctccttcc tgcccggtta ccacaggtct
    30841 gctggcatct gatatcaccc tcaactctgc agtgctgagc tgggatgata tgggcccgga
    30901 tgttcagggt tataagttcc agctgcagaa tattgctgaa ggaaccaacc gccgcaaaac
    30961 actggatgca ggtactactt cacttttcct cggaccactg gttctgtctc cgggcagtga
    31021 ctacgtgttt gcagtgaaaa cactgtgtct ggatggttca caatctgcct ggtcagaact
    31081 gtactatttc agcacagctt ctgctgaacg tatgaccgaa agcagtatga acctctcggt
    31141 atatccgaac ccttccaatg gcagtttcta cctgaatggt ccggtaacga acggtgctca
    31201 actcatcatc actgatctga gcggcaagac cgtatatact caggtgctgg atggttcagc
    31261 agaactgctg caaacagatc tggcagccgg tatgtaccat ctcgttatac tgggtggcga
    31321 tgaaaccttc cacaccagcc tggtgatcga ataagatatt tattcagaca aaaaaaagag
    31381 cgaccaacgg gccgctcttt ttttatgtct tagctgagaa ggtcactgca ccaggaacat
    31441 ctgttctgtg gttcctgatg attgcgtgat gcgcatcata tacgttccat gagcccaatc
    31501 tgaaacctgt acctgcatct ggccgttccc ggtagcctgc caaaccaact gaccgtctgc
    31561 agcaaagatg cttacagtgc ctgcaaccgc cgggatgtac aggatatcat gtgcggggtt
    31621 gggatagacc gtcagttcca cactgcccgt aagtctttgc accggaacga tctcactcca
    31681 ggtatgctgt ccgtcaaaat ctgtttgttt cagccggtaa taggaggtgc ctggcagcgg
    31741 ctcatcatct gtaagctggt agtggagggc tgtattactg ttgccggctc cggctactgt
    31801 tccaacaggt tcaaaataca ccccgtctcc actgcgttcc accgtaaaat gatcattgtt
    31861 gatctctgat gaagtggtcc agtgcaggat gacttcctcc ttctggttgt cagcagaaaa
    31921 attggtcagt acaatgggta gcggatacaa ttcactaaag ccgatggcaa aatctgaaaa
    31981 cgaagtagcg ccaaagttgg tcaccgtaac agggtctgtt ggcgatgtga tcaggggcgg
    32041 cagatctgct accacaccgg gttccaccca tccggtggca gacaacgcat tgctgttctt
    32101 gaatatgcca tgaaaatcca gatacggagc cgcattggta tgcccgcggc tggttagcgt
    32161 aatactgtag gtcactgagg taacggcatc cggatcgatg ttccagtaac cgtaattaag
    32221 gaaatcaatg acaggggtaa caatgccatc taccacaggt ccttcagaga acaaaccaac
    32281 agggcccacc ggatctgtcg ggtcaaagga cacggtaata ttattcagac ccacagctga
    32341 ggtgatctga atgaccgctt cttcatattg ggcagctgta ccaacaggga aagtataagt
    32401 agctcctgca ctcacatcac gtttcaggtt accgttcaca taggcattgg ttccgtaacc
    32461 actaatggtg gctgaaaaaa tactgttgat atataaagta ttagcaaatg tgttgatcag
    32521 ggtggaagat gcgaacacca atccgtcgct gatggccagg tcattggaca gatttacctg
    32581 ccctgccggt ttgttgatga tcagataaaa gaattgttct tcaaaaccgc tggtgctgat
    32641 ggtttgatct gctgaaccta gcagggtgat gaaggagatc gtttcatcaa agtctgcggc
    32701 actgctcagg ttggcatagt ctcctttcag ttcgaggaat gaaaaataca gcgacaggaa
    32761 accgcctgca tctatctgca gatcgccatc cactgctatc agcccgctgt gttcattcag
    32821 cagagatcca cctgattcaa tgaccacact gccacccacc agtaaggagt cgctggcatc
    32881 agctacctcc aggctaagac cgtttt
//
LOCUS       DAOMAX010000005        28856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_179_length_28856_cov_4.329051, whole genome shotgun sequence.
ACCESSION   DAOMAX010000005 DAOMAX010000000
VERSION     DAOMAX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28856
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_28856_cov_4.329051"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3760
                     /locus_tag="PK742_00815"
     CDS             <1..3760
                     /locus_tag="PK742_00815"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQU75158.1"
                     /translation="LDPGAFLLIYASGKDRKPDGGIDHWEGVVTSSTSWKYRVPNALT
                     PTDWREIGFDAASWSSGLSSIGYGDGDDATTVPAGTVSVFMRYEFTITDTSRVADAFF
                     HMDWDDGFVAYLNGTQIAQFGFPGGTPDYNDFSGSDHEAVMYGGGLPDAWALDEAWLK
                     TMLVEGENVLAVEVHNINSGSSDLTSRPFLTVGFKDADWTFDVPEIWFTEGSFANNLH
                     TNFKLDGDGEVVFLSYPDGSPADSLSVPAMFYDQSFGCSTDGAATHGIFIDATPGYTN
                     NGSSVYTGYTPEPAFSLEAGFYTGTQSTEITCPVPGMSIRYTLDGQTPTPSDLLYSGP
                     VEMDETTTVRAACFSTDPGLLPGRVATHTYFIDEETELAVISITTDDVNLYGGQGIYD
                     NWWTDWKKPCFLEYFNEEHEAVVGQYSGIKLEGGAGGSRSLPQKSFRLEPGSGAYGDG
                     VINYPLIPRVPASDTYDRFYLRNGSNMWNVLPYKDACMTRCTQGSHTEFMQYTPVEVW
                     LNGEYWGLYELREKLDAGHFQYTHLIEPENLDLLSMSYWYSLILRTLEGSADEWMEMV
                     DYLYYYPTPTDDDFWEVADSLLNLVDFTDYMIAETWMGNLDWPWNNIKIYRDRGGDNQ
                     WKYGVIDLEWGLGYGWSDESTDMIANILYNYNYYTSPFLSLIQNERYRNYFINRYADL
                     MNTIYLPERTLAVEDSIYDVVVTAMPRQLQRWGDGSPLWSQMATFEDYRGALLDDFEV
                     RSLYVRNHIWNNFDLDGKVDIEIHIEPPGAGRVHLNTIDIYDPAWEGVYFDGVPVTMT
                     AIPFTGYSFDHWGDSPYIDDESLPSFTVNFDDDDIVTAYFNGSPQPETIVVSEVSYHS
                     EETVDAGDWFELWNHGDHPIYLDNWMVRDDEPLHAFYFPYHTTLMPGERLVVSADPAQ
                     FQLMHPDVAVFGPCGFQLSNDGDMIQLFNDRGDEVFVMTFSDNLPWPVGADGEGRTLE
                     LRTADADPNLPASWFDGCIGGSPGAAYEPCGDPIVYSEVNYHPADDHDSDDWVELRNV
                     SEESVDLSGWTFLTGRISMLEAFTIPNGTILEPGDHYLLTQTDWKFEGLYLDVTADDG
                     SFFFDLDGGNEWLRMYDNSGVLQMSLHYFDESPWPLEADGLGYTLELIDSTGVVNDAA
                     NWTTGCLYGSPGTWMSLPCPESVAIDDPVAAGFHVYPNPAHDLVWIVLPYAAHWQCTL
                     TDLQGRIIGVAKLSGQNGNWQLPELNPGTYTLTIANDGEAFTRLLLIQ"
     gene            complement(3762..6026)
                     /locus_tag="PK742_00820"
     CDS             complement(3762..6026)
                     /locus_tag="PK742_00820"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQU75159.1"
                     /translation="MKTHYPAVLLACLLATSTISAQQFYIPLRTSEPVELDGLMAEPD
                     WQKTPVVSEFMQESPDPGLDATERTEVRIMYDDDYLYISVFAFDREPDKLIRTELQRD
                     FPLGNDDGTGVTIDTYHDKLTGMSFVTNTLNARWDAQITRDGDGMNSSFNTFWDARTT
                     IHDKGYHTEYRIPFSSLRFEPGKEVVMGIRIARLIKRKNELVAWPPCNPATGSIWNNV
                     SFAMPIKFTELKGKNPFYAAPYAIANFDDFTYLDMTDTSWQHQGVFLNRKHFSDNETL
                     DKIVSNIGLDVKYGVTRNFTLDLTLNTDFAQAEVDDRIINLTKYEVNLPEKRNFFLES
                     ANALSFGFPSGNEVFITRRIGNEAGTIVPILGGARVTGKSNGWLVGALNMQTTGVMAD
                     DTIHPHNFTVARVRKDIDSLGSFVGGILTNRYDTDGGGPSFQVFGADFVKRVSQQFAV
                     EGGFATSMAEFELTDMLKQTYMHIGTFKSASQGFTFYSTADLIGDDVQMVMGYLDDTG
                     YGRTVDGIQYQWQLADNEKLQYFYIATDNSFRWRTASGNVETLSNSARAGLVFENGAW
                     LEWMPLERKQDSLFFDWQLNESNAIAAGTYTMYNTYIGVTLPTQSTYNVSFESTYGDF
                     YGGKRIYASAYAQYYFNTHFNGSVNYEFNNIGFNQYLLTDSNTVYTSNLYRLSLNYNF
                     STRISLKAYIQYEDLSDLTSANFRFRYNPQEGTDLYLVVNEGLNSDRNRLDPRMPVLN
                     NQAVVLKFVKTFSI"
     gene            complement(6069..7139)
                     /locus_tag="PK742_00825"
     CDS             complement(6069..7139)
                     /locus_tag="PK742_00825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQU75160.1"
                     /translation="MAYLELNREALAHNYQFLADHFHKNQKHWGVVTKVLCGHKTYLE
                     EVLKLHTGTVFDSRLSNLKAIRKLQPETRTVYIKPPPKRIIPKLLEVADVSFNTQLST
                     IRLINEEAIRQDKIHQIIIMIEMGDLREGVMRDDILDFYANVFELSNIEVIGLGTNLN
                     CLNGVMPSYDKLIQLSLYKELIEATFHQKIEWVTAGTSVVFPLLEQGLVPTGMNHFRM
                     GETLYFGNNLITGEPLPGMRQDVFRLYAEIVEIMEKPEGLDQPTGVNAAGVTPDADQF
                     ERGRTRNRALIDTGLLDIHYDNLKPVLQDLSVEGASSDMLVVDVGNNDTNLNTGNHIA
                     FQVNYMGLLSLMNSRYIEKKLL"
     gene            complement(7126..8553)
                     /locus_tag="PK742_00830"
     CDS             complement(7126..8553)
                     /locus_tag="PK742_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQU75161.1"
                     /translation="MHPVDLGIFIAYMLLMLAIGWYFHRRNKGHDDYFAGGRNMKSWH
                     VGLSVVATDVGGGFSIGLGGLGYMIGLSGSWILFTGLLGAWMSAVFLIPVVHRTGAMN
                     HLLSFPQWLKHGYNKQVAFLAGLISAIGYLGFTSSQLLAGAKLASGVFTELELSSALL
                     IMGGIAVVYTALGGLKAVIYTDTVQWIILLGGLLFVGVPLGYHAIGGWEGIQASVPAH
                     FLQLNNIGVIDLINWGVTIIPIWFIGMTLYQRIYALPDVRSAKKAWFIAGIFEYPVMA
                     FLGVTLGLFSKIAAEQGLLAGVIGPDHSQLDAEMGLPVFLRTILPVGGMGLLLSAYFS
                     AILSTADSCLVAASGNMIHDLLPDRKPFNSLGFGQLLTGLTGMIAIVLALQMTSVLDL
                     MLYSYAFMVGGLFIPVIGLLVFSKPNPKAALWGMITGGVVTTAGAAPGVPIPWQLDPV
                     IPGILASSMLFLSIQKFGKQAYGIS"
     gene            complement(8560..9009)
                     /locus_tag="PK742_00835"
     CDS             complement(8560..9009)
                     /locus_tag="PK742_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU75162.1"
                     /translation="MSDDLQIEVYSAFRTPSVQQKESIADFLFQHLGKYGDRREDIMT
                     CLDYALKETPSFGGLIFVGIKQSETVGVVVVNKTGMSGYIPENILVYIATHSAHRREG
                     IGELLMRKVIERTEGDIALHVEPDNPARRLYERLGFGNKYLEMRLHK"
     gene            9244..11640
                     /locus_tag="PK742_00840"
     CDS             9244..11640
                     /locus_tag="PK742_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75163.1"
                     /translation="MNRILPLLLLTLAATSLMAQGTIVGKSSYDVQTNNGTKNRIRVY
                     DDGSVSAIWTGSTDYAVGTTWPDRGMFYNHSDGVTWGAVPAARVEANKTGFGELMQVE
                     DHEVIVAHDGIDMRLYANSSIGSNDWTELGGSGPGGMTGFWPMADCPAGTDDIYVIHA
                     NANPPTALYFSRSDDGGNSWSVLNYTLPLLDAAYGMPGMNTGLTTAAETYQIKVHGAD
                     VYVLFGIPNSDLVLLHSDNYGNDGSWERQVLIDFPFDEYTGTVQTDIDLDGITDTVDT
                     HDGYHDMIMEDDGILHVFSGYTRLYSDAGAFSYTYNYNAWGIWHWMTGMPSAELIDTR
                     LDWINDECDNDAEAGLGAYRFTYRYSSNTTSPGAAWDPLTGRIYLLYTMQIEYTDIYD
                     DPLNLSAQSRRDIFGMYSDDDGTTWTKPANLTMGAELDEENFFLFLNEKVVGGKVHAI
                     WQQDELPGSAAGEADPIDTNYIRYATWDAEAFAVTLPTADFTYTSDLSLFNFTDGSSD
                     NFPCYYWDFGDGETSDFVNPSHIYDTGGSYEVCLTVSNPYGSDTYCETVTVPGAPETS
                     FTWSGDPDVSFTDLTLNEPTYWSWNFGDGSTSTEQNPVHSYAFNGAYNVCLTASNALG
                     SDTYCATVSIGNAIGAPVADFTYTITGLAVELTDISSNAPDTWSWNFGDGTTSADQDP
                     SHTYAAAGTYNICLTVTNAVGGDTECKSVSTANAVTELVSFIQVFPNPTAGLVTVSWE
                     GTNPELLQLYDGQGRLVDVSAVLSANGAVLNMQPLASGVYQLQLIAGNEQAIISLIRE
                     "
     gene            complement(11643..13721)
                     /locus_tag="PK742_00845"
     CDS             complement(11643..13721)
                     /locus_tag="PK742_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQU75164.1"
                     /translation="MTQQDTAAMIDTQVLMQAYRKMYTSMVMSQVFDENRQICIYVHA
                     NSRGHEAIQLAAGMQLKSSDWASLYYRDDSILMGMGWEPYEIMLQQMAKRDDPFSGGR
                     NYYCHPSWRGKGKPQMIHQSSATGMQAIPSTGIAQGIQYMTEQGLLETAADNPIVLCS
                     IGDGAMTEGEVSEALQFAVLKELPVIYLVQDNDWGISVAGHEARVMNAYEYAAGFPGL
                     QRMQVDGSLFLDSWQTMEQAISYVRQHRKPVLVHAKVPLLGHHTSGVKKEMYRSTEDL
                     AAHALRDPLPKLRSLLIDMGVQTSELEAIESHLDTSVRNDFERVVASPDPDPETVADH
                     EFAPALMTEETGTREPEGAPVVIMVDAALHAMKEILAAHPEALFYGQDVGRRLGGVFR
                     EAATLADTYGDHRVFNTAIQEAYIVGSTAGMSAVGARPIVEIQFADYIWTGVNQLVSE
                     ISKSCYLTNGKWPVSAVIRIPIGAYGSGGPYHSGSIESSITAIKGIKVVYPSNAADMK
                     GLLKGAYYDPNPVIMFEHKGLYWSKVPGTQAAKTPDPADDYIIPLGKARIFQHADAGA
                     TDNGESMVVVTYGMGVHWAVNASRNFPGQVEILDLRTLYPYDWESVKQSVMRHGKCFV
                     LTEEPLINSFAQALAGRIGSECFTHLDAPVQTLGSLDLPAVPLNSGLEKAMLPNTDKV
                     SAVMADLLSW"
     gene            complement(13725..14333)
                     /locus_tag="PK742_00850"
     CDS             complement(13725..14333)
                     /locus_tag="PK742_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQU75165.1"
                     /translation="MKIVCIARNYVDHAKELNNAVPTTPVLFLKPDTALLQGNAFFMP
                     DFSADMHYETELVIRIGKNGKHIDESFALNYVDAITTGIDFTARDVQARQKEKGLPWE
                     IAKAFDQSAVVGSWQDADPVRNGRPVHFSMHRNGNLVQQGNSQDMIFSVARQISYAST
                     FFTLRQGDILFTGTPSGVGPVAPGDKLEGFLETDKVFDIQIR"
     gene            complement(14376..14948)
                     /gene="pth"
                     /locus_tag="PK742_00855"
     CDS             complement(14376..14948)
                     /gene="pth"
                     /locus_tag="PK742_00855"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQU75166.1"
                     /translation="MSERTFLIAGLGNPGSEYAGTRHNIGFDVVDLLCQKLEGNWEDK
                     RYGWRSECRLKGRKIILLKPSTYMNLSGRAILYWMKEYKLEKEQLLVITDDINLHTGK
                     LRLKTRGSDGGHNGLRNIQEMLMTTDYPRLRFGVGNDFPKGAQVHYVLGKWADEEKKL
                     VEEQLIKAAEAAESFVLEGIDRAMNRFNNA"
     gene            complement(15005..15598)
                     /locus_tag="PK742_00860"
     CDS             complement(15005..15598)
                     /locus_tag="PK742_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQU75167.1"
                     /translation="MKTVNIEGKVRTSTGKAASNASRKQGMVPCTLYGRENNISFEVS
                     ESALRGIIYTPEFFKVNVKLDGKEYETLIKDMQFHPVTDKPLHLDFFVLSSDKKVVCE
                     IPLQPKGLAAGVKAGGKLSQKIRKVKVKGFPKDLVDLIEVDVTHLEMGKSVRIGEIKV
                     DNLEIMGSQAIPVITCTIPRSMRSKQSAEAAEAKSAS"
     gene            complement(15609..16550)
                     /locus_tag="PK742_00865"
     CDS             complement(15609..16550)
                     /locus_tag="PK742_00865"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQU75168.1"
                     /translation="MVTNDVKLFSGTTSRYMAEKIADYYGQPLSRIQIDRFSDGEFQP
                     NILESVRGNYVFLIQSTYFPSDNLMELLLMIDAAKRASASYITAVIPYFGMARQDRKD
                     KPRVSIGSKLVAELLTAAGANRIVTMDLHADQIQGFFDIPVDHLSSTAIYVPYIEENL
                     DLKNVIFASPDVGSTKRNRHYAVHFNTELVICDKVRKKANEISEMTVIGDVTGKDVIM
                     IDDIVDTGRTLANAANIMIDKGARSVSAFCTHPVLSGEAYNTIEKSQLKELVVTDTIP
                     LRRESEKIRVLSSAKLFARAIRNTHEHRSISSLFINN"
     gene            complement(16648..16720)
                     /locus_tag="PK742_00870"
     tRNA            complement(16648..16720)
                     /locus_tag="PK742_00870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16685..16687),aa:Gln,seq:ttg)
     gene            complement(16769..17941)
                     /locus_tag="PK742_00875"
     CDS             complement(16769..17941)
                     /locus_tag="PK742_00875"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQU75169.1"
                     /translation="MASNSINIQKLAGTWRAALETPAGELPFQLEIYQSGTVWIAAVI
                     NGEEQLTTTEIYTDSGHITIRSDIFHSEIIAEADTSFNSMSGYWEDLSRKTGYRLPFR
                     ADKDVYHRFSEFPEEPTADIGGTWKVTFTSDSGGVTMAIGMFEQEGNHLSGTFLTNTG
                     DYRFLEGEVNGSKFALSAFDGSHAFLFTGEFDGTGCNGIFCSGIHWQEQFNMVRDHTF
                     VLDDPDMLTGFDGSEPLTFCFPDQQGNATSLTDECFRNKVIIVQIMASWCPNCMDESA
                     FLSSIYDSFQPEGLEIIAVGFERETAFKEVAENFDRLRKKLGIRYTMLLGGGAEKKEA
                     GAAFPTLSRILAYPTTLILDRDHRITAVHTGFSGPATGKIYEAFREKIIRHLHRVL"
     gene            17995..19029
                     /gene="mnmH"
                     /locus_tag="PK742_00880"
     CDS             17995..19029
                     /gene="mnmH"
                     /locus_tag="PK742_00880"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HQU75170.1"
                     /translation="MWPSVEPEKILHGKAQLVVDVRSPAEFAHAHIPGAISLPLFSNE
                     ERAVVGKIYKENGHDAAVSKGLSIAGPKAADLVDTIRRLAGDKEVLLHCWRGGMRSAS
                     VAMLLQLAGIRGRVLKGGYKAYRRMVLGAFEKPFRIQILGGYTGSGKTAVLRELAATG
                     QQVIDLEGLAKHKGSAFGNLEDAAQPGTEQFENDLFEQLQTIDPEKTLWLEDESHSIG
                     RVFIPEAWWRQMRQAPVLFLHIPVEARATWLVKDYTEAAPGVQAVEKALEKIRKRLGG
                     QHYQEALELFRNGLLKEAATKALYYYDKAYLYGLSSRAEDTITYQEEPMVDPLMISKN
                     IIEKYAYKFE"
     gene            19010..20062
                     /gene="selD"
                     /locus_tag="PK742_00885"
     CDS             19010..20062
                     /gene="selD"
                     /locus_tag="PK742_00885"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQU75171.1"
                     /translation="MHTNLNDIKLTAYSHGAGCGCKIAPAVLDEILSFAGKQGQFNAL
                     LVGNDTRDDAAVMDIGNGTAIISTTDFFMPIVDDARQFGRIASVNAISDVYAMGGKPL
                     LAIAILGWPIDKLPAEVAGLVLDGAREACTEAGIPLAGGHSIDSPEPIFGLAVTGQVS
                     HEHLKKNSGASAGVLLFLTKPIGVGILTTAQKKGKLQPEHAALAPAQMMQLNSIGEVL
                     GKLDYVEAMTDVTGFGLLGHLSEMCQGSDLSAEVFLDKVPFIDTAILDHYLSEGCVPG
                     GTNRNFKSYGHLVDGLNDRSRNLLCDPQTSGGLLVAVHPEGEEAFHQVMEQYDMKLTS
                     LGRLTERSSHVIHVYS"
     gene            20065..20463
                     /locus_tag="PK742_00890"
     CDS             20065..20463
                     /locus_tag="PK742_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75172.1"
                     /translation="MKYPHIFLLLLMAGCTKDEPLLDFYNSTYSGTVTYHSSTTISGV
                     TTTNDSTYSETIILSMIGEDSLNISSPSFGERKVLMILHSTEYITYGSAYSFSYYIGS
                     DFIKLEDHSFSGGAGGFGSTVDRSLSAYRD"
     gene            20524..21819
                     /locus_tag="PK742_00895"
     CDS             20524..21819
                     /locus_tag="PK742_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQU75173.1"
                     /translation="MTQTSMSAPSVPQVSQMAANLIGSEIIKLAADINKRIQQGEQIY
                     NFTIGDFDPQVFPIPEALEEKIIQAYRSKQTNYPAANGIEPLRKAVSHFLEKGLGLTY
                     SPDDILISGGGRPLIYAAYMSVVDPGDKIIYPLPSWNNNHYTHLSGGVPVEIETSAAN
                     HFMPTADDLKPHIGDATLIALCSPLNPTGTTFSKEALTSVCELVVEENARRAGKQKPV
                     YLLYDQLYWTLTYGETMHHDPVSLVPGVRPYTIYIDGLSKAFAATGVRVGWSAGPPHI
                     IAKMRAILSHIGAWSPKPEQVAAAAFLEDDEAVQSYLETYKAALHQRLEGFYRHFQHL
                     KASGFPVDCIAPQAAIYLTVRIDLRGATTQSGEQLTDMAAVTRYLLQDAGLAIVPFYA
                     FGTGRESPWFRLSVGTCRMEDVDASMEKLGAALRSLQFS"
     gene            complement(21820..22209)
                     /locus_tag="PK742_00900"
     CDS             complement(21820..22209)
                     /locus_tag="PK742_00900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQU75174.1"
                     /translation="MHTTEITVRGFDCDIYAHVNNARYLEYLEACRWDWVNATGATEY
                     FARRKMSFVVVNINIDYRRPAVLNDVLLVSVRKDEVGNKSARVKQTVIRKSDDKLIAE
                     ATITFAMVDNTTGKTVEITEDMLPYFD"
     gene            22251..23114
                     /locus_tag="PK742_00905"
     CDS             22251..23114
                     /locus_tag="PK742_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU75175.1"
                     /translation="MYDQPMLRDNALKGKTFIVTGGGTGLGRSMSKYLMELGASVVIS
                     SRKLDVLQKTADELQAETGGKALAVACDVRIYEEVEQMLQQAVDAFGQVDGLLNNAAG
                     NFISPTERLSHRAFDTVVDIVLKGSYNCTLALGKYWIEKKISGTVLSITTTYAFTGSG
                     YVVPSACGKAGVIALTRSLAVEWAKYGIRLNAIAPGPFPTEGAWSRLVPPGLEDDLDM
                     KKKVPVGRVGHHQELANLAAYLMSDFSAYVNGEVIVIDGGEWLKGAGEMNVLEKVPDQ
                     LWDMMEKMRKK"
     gene            complement(23179..24210)
                     /gene="murB"
                     /locus_tag="PK742_00910"
     CDS             complement(23179..24210)
                     /gene="murB"
                     /locus_tag="PK742_00910"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQU75176.1"
                     /translation="MTSKLPSHNASLAGLHTFGIDCKASLLFTVKQQDDLQVLQELLR
                     QYPTFLILGGGSNILFTKDYPGLIVLNRIKGISAQPDPDNDRFVLLTAAGGEVWHDVV
                     LYAVQKGWGGLENLSLIPGTVGAAPIQNIGAYGVELKDVFHHLEALDLETGELHTFDK
                     ANCMFGYRNSIFKHELAGKYLITSVTLRLDTRPELRLSYGAIQDTLDAMGITEPNIAD
                     VSQAVISIRQSKLPDPAELGNAGSFFKNPEISRKHYEQLQMEYPDIPGYPGSAADLVK
                     VPAGWLIETCGWKGRRTGNTGAHSKQALVLVNYGNATGIEIWEHAQRIMRNIKERFEI
                     SLTPEVNVI"
     gene            24281..25765
                     /locus_tag="PK742_00915"
     CDS             24281..25765
                     /locus_tag="PK742_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75177.1"
                     /translation="MRLFVLILTLIFGISELSAQAIPLISPDRDMTFTKRNTLDEDSA
                     YFFIRTTSVADEADGTAYYFNVNIDTAGGGCETDTTFMGRKALLLDDIYQTYVFFNDQ
                     QDSIFIRTDVELYDSWVFYTWPDGKYIKAQANNKIFKPIMPGVNDTIVRIKLSVFDPS
                     NNPLDIWPDNQKFDLSEQYGLAELYNFYAFPNDQELILLRGVSNPDTAITDVSAHAAL
                     DIAPGYEMHYEEFFEDEGYFDLHRLYKLFVLDTLHNDLSVVITSQRTLWQELTLSGTT
                     DTLQVLDTITVEYFYSDLAQLDTPELQWMQYEEGAFAFSDFILEPDTYSGIRHKWLYG
                     GYSSTSEDTLCASILTGASPWPLKLYGNELGIMYKLDSVLELLVPDAIDPDYGNVELQ
                     MVYFQKGLITWGEPIDFYELGFTGINEPAELAFQIYPNPAEDHLQISGVQQAAYINIY
                     SADGRLLRSEPYYSYISISELPSGIYLLQIMSDEGTGTTTFIKQ"
     gene            complement(25771..27249)
                     /locus_tag="PK742_00920"
     CDS             complement(25771..27249)
                     /locus_tag="PK742_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75178.1"
                     /translation="MRSRFLLLLLLAVFCRPLVSIAATYEVNQRCEDAMNMVMALRFD
                     EAKKIIAAERKANPGNLFPYYIENTMDIISIYISEEEARFDELEPHKDIYLSKLKSGD
                     ASSPYYLYLQAEVNIQWAFARLKFEEYLNAFNEIKRAYGMLKDNDEDFPDFLPNQKSL
                     GMLHCLFGAIPDQYKWGANILGLSGDIDAGLKEMRSFLDKPGDFPQIFVQETTLYYAF
                     LVLYLQKDYNKAWNIVKDLDTRNNMLFCFCKASVALRTGRCDAAISTLQSRPTGNNYF
                     PFPFMDFMLGLAKTRRLDSDANRYFESFLVQFKGKNYIKEAYQKMGWNALIAGDMSRY
                     KFYMERVKLYGDDVIDDDKQALLEAESGIPPNVTLLKARLLFDGGYYKTAIGQLEGLS
                     TDNFKSDKDKTEFTYRAGRIYHESGNPEKAKGFYAATIKNGGKLPYYYAASAALQLGL
                     IYEHEGNKEKAAYYYNACLNMPNKEYETSLDSQAKAGLNRIK"
     gene            complement(27294..28037)
                     /locus_tag="PK742_00925"
     CDS             complement(27294..28037)
                     /locus_tag="PK742_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HQU75179.1"
                     /translation="MATVKELTIKESLQHLYNLQKIHTKIDEINILKGELPMEVSDLE
                     DEVAGLETRIHKLEAELKEVDDQIAERKNAITDGKANIKKYEKQQDNVKNSREFDALT
                     KEVEMNKLDIELSEKKIKELTASKDARKEAIDTSKINIEHKKEDLAKKKEELEHIQKD
                     TEKEEKELEKKVAAAEKGLEERLLAAYYHIRNNYRNGLAVVTVERNACGGCYAEVPPQ
                     RQSEIKQAKKIIICEHCGRILVDDSISED"
     gene            complement(28040..>28856)
                     /locus_tag="PK742_00930"
     CDS             complement(28040..>28856)
                     /locus_tag="PK742_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQU75180.1"
                     /translation="AIHTNLDNVRGGVNQAIAAKLQLQETRVLRPIKGLLKKLVTFAP
                     EAHAAEVRFALFAAGAGDIGAYDHCSFNTAGEGTFRAGESASPFVGIKGEDHLEAEVR
                     IEVIFEQWKEAAILNSMREAHPYEEVAYDIYPLDNALQETGAGLVGELAEPMDVNSFL
                     QYLKDQMQTPVIRYAGSTHRSIKKVAVCGGAGGFLLPDAIRTGADCFVTADLKYHQFF
                     DAEDVIILADMGHFESEQFTSDLIIGFLNQKFANFAPSFPIVKGMDTNPVKYF"
BASE COUNT         7172 a         6855 c         7131 g         7698 t
ORIGIN      
        1 cctggatccg ggggcatttc tgcttattta tgccagcggc aaggatagaa aacccgacgg
       61 cggaattgat cattgggagg gtgtggtcac ctcatccacc tcctggaaat atcgtgttcc
      121 gaatgcattg acaccgacag actggagaga gatcgggttt gatgcagcct cctggtcttc
      181 aggcctttcg agcatcggct atggagacgg agatgacgcc accacggttc ctgcaggtac
      241 cgtgtctgtc ttcatgcgct atgaatttac cattactgac acttcgcggg tggcagatgc
      301 tttcttccat atggactggg atgatggctt tgtggcttat ctgaatggta cacaaatagc
      361 acagttcggg tttccgggag gaacacctga ctataacgat ttttcaggaa gcgaccatga
      421 agcagtgatg tatggcggcg ggctaccgga tgcctgggca ctggatgaag cctggctgaa
      481 gaccatgctt gtggaagggg aaaatgtatt ggcagtagag gtgcacaata tcaattcggg
      541 tagcagtgac ctgacctcac gaccttttct gaccgtcggg ttcaaagatg cagactggac
      601 ctttgatgta ccggagatct ggttcacgga aggaagcttt gccaataacc tgcataccaa
      661 tttcaaactg gatggagatg gcgaggtagt atttctgtca tacccggatg gcagcccggc
      721 tgattctctg tctgtaccgg ccatgttcta tgaccaatct ttcgggtgca gcactgacgg
      781 ggctgctaca catggaatat ttattgatgc aacacccgga tacaccaaca acggatcttc
      841 cgtttatacc ggttatactc cggagcctgc tttttcactt gaagccggct tttataccgg
      901 tacacaatct actgagatca cctgcccggt tccgggaatg agtatcagat atacgctgga
      961 tggtcagaca cccacaccat ctgatctgct gtattccggc ccggtagaaa tggatgagac
     1021 cacaactgtt cgtgctgcat gtttcagcac agacccgggc ctgcttccgg gacgggtggc
     1081 cactcacacc tatttcattg atgaagaaac agagctcgct gtcatttcca tcacaacaga
     1141 tgatgtcaac ctgtatggcg gacaaggtat ctatgacaac tggtggactg actggaagaa
     1201 gccctgtttc ctggagtatt ttaatgaaga acatgaagct gttgttgggc aatacagcgg
     1261 tatcaagctg gaaggtggtg ccggtggcag tcgttctctg ccacaaaaaa gtttccgtct
     1321 cgagccgggt agtggtgcct atggcgatgg ggtcatcaac tacccgctga taccccgcgt
     1381 tcctgccagc gatacctatg atcggttcta cctgcgcaac ggcagtaata tgtggaacgt
     1441 gctcccttac aaggatgcct gtatgacccg atgtacccag ggaagccaca ccgaattcat
     1501 gcaatacacc ccggtggaag tatggctgaa cggtgaatac tgggggcttt atgaactacg
     1561 ggagaaactg gatgcaggtc attttcagta cacccatttg atagaacccg agaacctgga
     1621 tcttctgagt atgagctatt ggtacagcct gatattacga acactggaag gctctgcaga
     1681 tgaatggatg gagatggtgg attatctcta ctattatcct acaccaaccg acgatgattt
     1741 ctgggaggtt gcagatagtc ttctcaatct tgttgatttc actgactata tgatcgcaga
     1801 gacctggatg ggaaacctgg actggccctg gaacaacatc aagatctacc gcgaccgtgg
     1861 gggagacaat caatggaagt atggggtcat cgatctggaa tggggcttgg gttacggttg
     1921 gagtgacgaa tcaacagata tgatagcaaa tatattgtat aattataact attatacatc
     1981 gccatttctg tctctgatcc agaatgaacg ctacagaaat tattttatca accgttatgc
     2041 agatctgatg aataccatct atctgccgga gcgtacactc gctgtagaag acagcatcta
     2101 tgatgtagta gtgacagcta tgcccaggca attgcaaagg tggggtgatg gcagtccgtt
     2161 gtggtcgcag atggcaactt ttgaagatta tcgcggtgca ctgctggacg actttgaagt
     2221 gcgaagcctg tatgttcgca accatatctg gaacaatttt gacctggatg gcaaggtgga
     2281 tattgagatc catattgaac cgcccggagc gggaagggta cacctcaata ccattgacat
     2341 ttatgaccca gcctgggaag gtgtgtactt tgatggtgtg cctgttacca tgaccgcgat
     2401 accttttacc ggatacagtt ttgatcactg gggcgacagt ccttatatag acgatgaatc
     2461 attgccttcc tttacggtca attttgatga cgatgacatt gtaaccgctt atttcaatgg
     2521 ttctccgcag ccggaaacca tagtagtgag cgaagtgagc tatcattcag aagaaacagt
     2581 agatgcgggc gactggtttg aattgtggaa tcacggcgac catcccatat atctcgacaa
     2641 ctggatggtg cgtgatgatg aaccattgca tgccttttac tttccgtatc atactacatt
     2701 gatgcccggc gaaaggttgg tggtcagtgc agaccctgct cagtttcagc tgatgcatcc
     2761 ggatgttgca gtttttggtc catgcgggtt tcagctgtca aatgatggtg atatgatcca
     2821 gctgttcaac gacaggggag atgaagtatt tgttatgact ttcagcgaca acctgccctg
     2881 gcctgttggc gcagatggtg aaggccgcac cctggaactc agaactgcag atgcagaccc
     2941 caaccttcct gcatcctggt ttgacggatg tattggtggt tcgccgggtg ctgcctatga
     3001 accatgcgga gacccgattg tgtacagtga agtgaattac catcctgctg atgatcatga
     3061 cagcgatgac tgggtggaac tgcgaaatgt atctgaagaa tctgtagatc tgagcggatg
     3121 gacctttttg accggtcgta tcagtatgct ggaggcgttc accattccaa acggtaccat
     3181 actggaaccg ggcgatcatt atttgctgac gcagacagac tggaaatttg aaggactata
     3241 tttggatgta acagcagacg atggttcgtt cttctttgat ctggacggag gcaatgaatg
     3301 gttgcgcatg tatgacaaca gcggggtatt gcaaatgtcc ctgcactact ttgatgaatc
     3361 accctggcca ctggaagccg acggacttgg atacacactg gagctgatag attctacagg
     3421 tgtggtgaat gatgcagcta actggaccac cggttgttta tatggttctc cgggcacatg
     3481 gatgagttta ccctgcccgg aatctgttgc cattgatgat cctgtggctg ccggttttca
     3541 cgtctatcct aatccggcgc atgatctggt atggatagtg ctaccttatg cagcccactg
     3601 gcagtgcaca ctgacagacc ttcaggggcg catcattggt gttgcgaaac tgtccggaca
     3661 gaatggcaac tggcaattac ccgaactcaa tcccggcacc tacacattga ccattgccaa
     3721 tgacggtgaa gcatttacca gactcctgct gatacaatag ctcagatcga aaaggtcttg
     3781 acaaacttca gaaccactgc ctgattattt agaacgggca ttctcggatc cagtctgttc
     3841 cggtcgctgt tcaggccttc gttgaccacc agataaagat cagtgccttc ctgagggtta
     3901 tacctgaagc ggaagtttgc actggtcaga tcactcaggt cttcatattg aatgtaagcc
     3961 ttgagtgaaa tgcgggtaga gaaattatag ttcagactca gccggtacag gttgctggtg
     4021 taaacagtat tactgtcagt tagcaggtac tggttgaaac ctatgttgtt gaattcgtag
     4081 ttgaccgacc cgttgaagtg cgtattaaag taatactgag cgtaggcact tgcataaatt
     4141 cttttaccgc cgtaaaaatc gccgtaagta ctttcaaagg aaacattata ggttgactga
     4201 gtaggcagcg taacacctat ataggtattg tacatggtat aggtaccggc agcaatggca
     4261 ttactttcgt tgagttgcca gtcaaagaac aggctatcct gttttctttc aaggggcatc
     4321 cattccagcc acgctccgtt ttcaaatacc agtccggccc gggcactatt ggaaagtgtt
     4381 tccacgttgc cggaagcggt tcgccatctg aaactgttgt cggtagcaat ataaaagtat
     4441 tgtagctttt cattgtcggc cagctgccat tggtattgta tgccatcaac tgttcttcca
     4501 tagccggtat catccagata gcccatgacc atttgtacgt catcaccaat gaggtctgca
     4561 gttgaataga aagtaaatcc ctggctggca gatttaaatg tgcctatatg catatatgtt
     4621 tgtttcagca tatcagttaa ctcaaactct gccatgctgg ttgcaaatcc accttccaca
     4681 gcaaactgtt gtgatacccg tttgacgaaa tcagcaccga acacctgaaa ggagggtccg
     4741 cctccgtcgg tatcatatcg gttggtgagg atgccaccca caaaacttcc cagtgagtcg
     4801 atgtcttttc tcactctggc taccgtgaag ttgtggggat ggatggtgtc atctgccatc
     4861 actcctgtgg tctgcatatt cagggctcca acaagccagc cgttgctttt acctgttact
     4921 cttgccccac ccagaatggg aacgatggta ccggcttcat tgcctatcct tcgggtgatg
     4981 aagacttcgt ttcctgaggg aaaaccaaaa ctgagtgcat tggctgattc caggaagaaa
     5041 tttctcttct ccggcaggtt cacttcatat ttagtgaggt tgatgatgcg gtcatctact
     5101 tctgcctggg caaaatcagt gttcagtgtg agatcgaggg tgaaattcct ggtcacaccg
     5161 tatttgacat caaggcctat gttgctgact attttatcta aagtttcatt atctgaaaaa
     5221 tgctttctgt tcagaaagac accctggtgt tgccaggagg tatctgtcat gtcgagatag
     5281 gtgaagtcat cgaagttggc aatggcatat ggtgctgcat aaaaggggtt cttgcctttc
     5341 agctcagtga attttatggg cattgcaaat gatacgttat tccatatgga acccgttgca
     5401 gggttacagg gaggccaggc tacaagttca tttttacgtt tgatcagtct ggctatccgt
     5461 atgcccatga ccacctcctt acccggttca aatcgcagcg atgagaaagg tatcctgtat
     5521 tcggtatgat agcctttatc atggatggtg gtgcgggcat cccagaaggt attgaaggag
     5581 ctgttcatgc catcaccatc acgggtgatc tgtgcgtccc acctggcatt gagagtattg
     5641 gttacgaaag acatgccggt aagcttgtcg tggtaggtat ctatggtcac acctgtacca
     5701 tcatcattac ccagcggaaa atctctttgc agttctgtcc ggatcagttt gtccggttca
     5761 cggtcaaatg caaatactga aatgtacagg taatcatcat catacatgat gcgcacttct
     5821 gtacgctcgg tggcatcgag acccgggtcc ggactttcct gcatgaattc gctcactaca
     5881 ggtgtttttt gccagtccgg ctctgccatc aggccatcca gttctaccgg ttcgctggtt
     5941 cgcaacggaa tatagaattg ttgtgccgag atggtgctgg ttgccagcaa acatgcgagc
     6001 agcacggccg ggtagtgggt tttcattcat taaatctaat cggaattttc aagaaaacga
     6061 actggcattc acagcagctt cttttcgata taccggctgt tcataagact caaaagtccc
     6121 atatagttga cctgaaaggc aatatgattg cctgtgttca gattggtgtc attgttcccg
     6181 acatctacca ccagcatatc agaactggca ccttcaacag aaagatcctg taacacgggt
     6241 ttcagattgt catagtgaat gtccagcagt ccggtatcga tcagtgctct gttcctcgtt
     6301 cttcctcgtt caaactgatc tgcatcaggt gtaacgccag cagcgttgac accagtcggc
     6361 tggtcaagcc cttcaggttt ttccatgatc tccacgatct ctgcatataa cctgaaaaca
     6421 tcctggcgca ttcccggcaa cggttctcct gtgatcaggt tatttccaaa gtacagggtc
     6481 tcacccatcc tgaaatgatt catacctgtt ggcacaagtc cttgttccag cagtgggaat
     6541 actactgagg tgcccgctgt tacccattct attttttggt gaaatgtagc ttcgatcagt
     6601 tccttatata ggctcaactg gatgagttta tcatagcttg gcatcactcc attcagacag
     6661 ttcaggttgg tgcccagtcc gatcacttca atgtttgaaa gttcaaatac attggcgtaa
     6721 aagtctaaga tatcatcacg catgacccct tcccgaaggt cacccatttc gatcataatg
     6781 atgatctgat gaattttatc ctgtctgatg gcttcttcat tgatcagcct gatggtagag
     6841 agttgtgtgt tgaaactgac atcggctact tcaagcagtt ttggaatgat gcgtttcggt
     6901 ggaggtttga tatagaccgt acgggtttca ggttgcagtt tcctgatggc cttcaggtta
     6961 cttaatctgg aatcaaacac agttccagtg tgtaatttca atacttcttc cagataggtc
     7021 ttatgcccgc aaagtacctt ggtcacgaca ccccagtgtt tctggttctt gtgaaaatgg
     7081 tcagccagga actgatagtt gtgtgcaagt gcctcccggt ttagctcaag atatgccata
     7141 cgcctgtttt ccgaattttt gaatggaaag gaacagcatg gatgaggcca gtatgccggg
     7201 aatgaccggg tctagttgcc atggaatcgg cacaccggga gcggcacctg cggtagtaac
     7261 cactcctcct gttatcatac cccagagggc agctttagga ttaggcttac tgaataccag
     7321 caagccgatc actggtatga acaacccacc caccataaag gcatacgaat aaagcatcag
     7381 atcgagtaca cttgtcattt ggagggccag cactatggcg atcattcctg tcagcccggt
     7441 cagcagttgg ccaaacccta atgaattaaa tggcttgcgg tcaggtagca gatcatgtat
     7501 catatttcct gatgctgcca ccaaacagga atctgccgtg gagagtatcg ctgaaaaata
     7561 ggcagacagc agtagtccca ttccaccaac aggtaagatg gtgcgcagaa ataccggcaa
     7621 tcccatttca gcatccagct ggctatggtc agggcctatc acaccggcaa gcaggccctg
     7681 ttctgctgcg atcttgctga acagcccaag cgtgactcca agaaaggcca tgaccggata
     7741 ttcaaaaatt ccggcaatga accaggcttt cttagctgac cgcacatcag gaagtgcata
     7801 aatgcgctga tacaaggtca ttcctatgaa ccagatcgga atgatggtca cgccccagtt
     7861 gatgaggtcg atcactccaa tgttattcag ctgaagaaag tgtgccggta cagaagcttg
     7921 tatgccttcc catccaccaa tagcatggta ccccagcggc acaccaacaa agagcagtcc
     7981 acccagcaaa atgatccatt gcacagtgtc tgtgtagatc acagccttta aacctcccaa
     8041 agcggtatag accacggcaa tgccacccat gatcagcagg gcagaagata attccagttc
     8101 agtaaataca cctgaggcca gtttggctcc tgccagcaat tgagaagaag taaaaccaag
     8161 atatccgatg gcactgatca gccccgccag gaaagcgacc tgtttgttat aaccgtgttt
     8221 cagccattgc ggaaaactaa gcaggtgatt catggcaccg gttcgatgta ccaccgggat
     8281 cagaaagact gcactcatcc atgcacccaa taatccggtg aacaggatcc aggaacctga
     8341 cagaccgatc atatatccca gcccacccaa accgatgctg aaacctccgc ctacatcggt
     8401 tgctaccaca ctgagcccta catgccagct tttcatgttt ctaccgccgg cgaagtagtc
     8461 gtcatgacct ttgttacggc gatgaaaata ccacccgata gccagcatca gcagcatgta
     8521 tgcaataaaa atacccaggt caacaggatg catgaattcc tatttgtgca gacgcatttc
     8581 cagatattta tttccaaaac ctaagcgctc atacaggcgt ctggctgggt tatcaggttc
     8641 tacatgaagc gcaatgtcgc cttcggtcct ttcgatcact tttcgcatca gcagttcacc
     8701 gatcccttcc ctgcgatgag cgctgtgggt ggcaatatac accagtatat tctccggtat
     8761 atatcctgac attccggtct tgttgactac cactacgcca acagtttcag attgtttgat
     8821 gcccacaaat atcagcccgc caaatgaggg tgtttccttg agggcataat ccagacaagt
     8881 catgatgtct tcacggcgat cgccgtattt gcccagatgc tgaaacagaa aatctgctat
     8941 tgattccttt tgctgaacgc tgggtgtacg aaatgcactg tacacctcga tttgaaggtc
     9001 gtcagacatg aaacaggatt ttttggcaaa gaatattgcc ggtgagatag tgagacaaag
     9061 atagttcaac cggaatgtaa tattgaataa aaattacaga tattatactg ctaaatatat
     9121 aaatatcatt ttcatgtttg cacataaccg tccaatgcct gttccttcga tcctgatatt
     9181 tttcaaattt ggatttgtaa gtctcagaac ctcttggtaa gttaggaggg aaaaccacct
     9241 cctatgaatc ggatcttacc cctgttgctg ctgacactgg cagcaacttc cctgatggca
     9301 caaggtacca ttgtcgggaa atcctcttat gatgtgcaga ccaataatgg caccaagaac
     9361 cgcatcaggg tatatgatga tgggtctgtc tctgccatct ggacaggttc tactgactat
     9421 gcggtaggta ctacctggcc ggacagaggt atgttctaca accactctga cggtgtcacc
     9481 tggggtgcag tgcctgctgc cagggtggag gctaacaaga caggattcgg agaactgatg
     9541 caggtagaag accacgaggt tattgtggca catgatggaa tcgatatgcg actgtacgcc
     9601 aacagttcca tcggcagcaa cgactggacc gaactgggcg gaagcggtcc gggaggtatg
     9661 acaggtttct ggcctatggc cgattgccct gcaggtacag atgatatcta tgttatacac
     9721 gcaaatgcca atccaccaac tgccttgtac ttttccagaa gtgatgacgg aggcaacagc
     9781 tggtcagtac tgaattatac cctgcctttg ctggatgccg cttatggcat gccgggtatg
     9841 aacaccggcc tcactacggc tgcagaaacg tatcagatca aagtgcacgg agcagatgtg
     9901 tatgtgctgt ttggaattcc caattctgat ctggtgctgc ttcattctga caactatgga
     9961 aatgacggca gctgggaaag acaggtgctc atagattttc ctttcgatga atataccggc
    10021 actgttcaaa cagacatcga tctggatggc ataactgata cggttgatac acatgatggc
    10081 tatcatgata tgatcatgga agatgacggt atcctgcacg tattctcagg ttatacacgc
    10141 ctgtacagtg atgccggggc tttcagctat acctataact ataatgcctg gggtatctgg
    10201 cactggatga ccggaatgcc atcggcagaa ctcattgaca ccaggctgga ctggatcaac
    10261 gacgaatgtg ataacgatgc agaagccgga ctgggggctt acaggtttac ctatcgctat
    10321 tcttccaaca ccacttcgcc gggtgcagcc tgggatccgt taaccggaag gatatacctg
    10381 ctgtacacca tgcagatcga gtacacagat atctatgatg atccgctgaa cctgagtgca
    10441 cagtcacgtc gtgatatttt cggaatgtac tctgatgatg atggaactac ctggacaaag
    10501 ccggccaacc tgacgatggg agcagaactg gacgaagaga attttttcct gttcctgaac
    10561 gagaaagttg tgggtggaaa ggtgcacgcc atctggcagc aggatgaatt gccaggaagt
    10621 gctgccggag aggcagatcc gatcgataca aattatatca gatatgccac ctgggatgca
    10681 gaagctttcg cggtcactct gccaacagct gatttcacct atacatcaga tctttctctg
    10741 ttcaatttta cagacggttc cagcgataat ttcccctgtt actattggga tttcggtgat
    10801 ggagaaacga gtgatttcgt aaacccatcg catatctatg acaccggagg cagttatgaa
    10861 gtttgtctga ccgtaagtaa tccttatggt tctgatacat attgtgaaac ggtaacagtt
    10921 cctggtgcac cggaaaccag ttttacctgg tcaggtgacc ctgatgtgag ctttacagat
    10981 ctgaccctga atgagccgac ttactggtca tggaatttcg gcgatggcag caccagcaca
    11041 gagcagaatc ctgtgcattc ttacgcattc aacggtgctt acaatgtttg cctcacggct
    11101 tccaacgctc tgggaagtga tacctattgc gccacagtaa gtatcggcaa tgccatcggt
    11161 gcaccggttg ctgattttac ctacactatt accggtctgg cagttgaact gacagacatt
    11221 tcttccaatg cccctgatac ctggtcatgg aattttggcg atggaaccac atctgcagat
    11281 caggatccca gccataccta tgctgctgca ggcacctaca acatctgtct gacagtaacc
    11341 aatgcagtgg gtggcgacac agagtgtaag agtgtgagca cggccaatgc ggttaccgaa
    11401 ctcgtatcgt ttattcaggt atttccaaac ccaacggctg gactggtcac tgtgagctgg
    11461 gaaggcacaa acccggaact gctgcagttg tatgatggtc agggaagatt ggtggatgtg
    11521 tctgcagtac tgtcagctaa cggtgctgtg ctgaatatgc aaccactggc atcaggtgtg
    11581 taccagttgc aattgatagc gggaaatgaa caggcaatta tttccctgat aagggaatga
    11641 tattaccacg acaacagatc tgccatgact gccgatacct tatccgtatt gggcagcatg
    11701 gctttttcaa gtccgctgtt caagggaact gcaggtagat caagagagcc aagggtctgt
    11761 accggcgcat ccaggtgggt gaaacattcg ctgcctatac gacctgcaag cgcctgtgca
    11821 aaactgttga tgagtggttc ttctgtcagc acaaaacatt ttccatggcg catcactgat
    11881 tgttttacag attcccagtc gtatggataa agggtgcgca ggtccaggat ctctacctgg
    11941 cccgggaaat tcctggaggc attcaccgcc cagtgaacgc ccatgccata ggtgaccacc
    12001 accatgctct caccattgtc tgttgcgcct gcatccgcat gctggaagat acgggcctta
    12061 cccagcggaa tgatgtaatc atcggcagga tcaggagttt tggcagcctg ggtaccggga
    12121 actttactcc agtagagacc cttatgttcg aacatgataa ccgggttagg gtcatagtag
    12181 gctcctttca gcagaccttt catatctgca gcattggaag gataaaccac tttgatgcct
    12241 ttgatggcgg tgatgctgga ttcaatggaa ccactatgat acggaccacc ggaaccgtat
    12301 gcgcctatcg gaatacgtat cactgcgctg accggccatt tgccattggt cagatagcag
    12361 gacttggata tttcggagac cagctgattg acaccggtcc atatataatc tgcgaattgt
    12421 atctccacga tcggacgggc accaacagca ctcataccgg cggtgcttcc cacgatatag
    12481 gcttcctgga tggcagtatt gaagacccgg tggtcgccgt aagtatcagc cagcgtggca
    12541 gcttcgcgaa atacacctcc taatctcctt cctacgtcct gaccatagaa caatgcttcg
    12601 gggtgtgcgg ccagtatttc cttcatggca tgcagggctg catctaccat aatgaccacc
    12661 ggtgctcctt caggttctct tgtgccggtc tcttctgtca tgagtgcggg ggcaaattca
    12721 tgatcggcaa cagtttccgg atccggatca ggagaagcta ccacccgttc aaagtcattt
    12781 cttacggaag tgtcaagatg agattctatg gcttccagtt cggatgtttg cacgcccatg
    12841 tcaataagca gactgcgcag tttgggcaac gggtctctca gtgcatgtgc tgcaagatct
    12901 tctgtactac ggtacatttc ttttttaact cctgaggtat ggtgtccaag caatggcact
    12961 tttgcgtgca ccagtaccgg ctttctgtgt tgccttacgt agctgattgc ctgttccatg
    13021 gtctgccagg aatccaggaa taaagacccg tcaacctgca tgcgttgaag accggggaaa
    13081 cctgctgcat attcataggc attcatgaca cgtgcctcat ggcctgctac agagatgccc
    13141 cagtcattat cctgcaccag atagataaca ggaagttctt ttaaaactgc aaactgcagg
    13201 gcttctgata cttcaccttc ggtcatggcg ccatcgccta tagaacacag tactatcgga
    13261 ttatctgcgg cagtttccag caatccctgt tcggtcatat actgaatacc ctgagcgatg
    13321 ccggtagatg gaatggcctg catacctgtt gcagagctct ggtggatcat ctggggtttg
    13381 ccttttcccc gccaggaagg atgacaataa tagttcctgc cgccggaaaa aggatcatcc
    13441 cgcttggcca tttgctgcag catgatctcg tatggttccc agcccatgcc catcaggatg
    13501 ctgtcatcac ggtaatacag actggcccag tcactgcttt tcagctgcat gccggcagcc
    13561 agttgaatgg cttcatgacc gcggctgttt gcatgcacat agatacagat ctgccggttc
    13621 tcatcgaata cctgactcat gaccatactg gtgtacattt tccggtaggc ctgcatcagt
    13681 acctgtgtat ctatcatggc tgctgtatcc tgctgtgtca ttacttatct gatctgaata
    13741 tcaaatactt tatctgtttc cagaaagcct tccagtttgt cgcccggagc tacaggtcct
    13801 acacctgagg gagtaccggt aaacagaatg tctccttgcc ggagggtgaa aaaagtggag
    13861 gcgtagctga tctgtctggc tacagaaaag atcatatcct ggctgtttcc ctgttgcaca
    13921 aggttaccgt ttctgtgcat ggaaaaatgc accggcctgc catttctgac cggatcagca
    13981 tcttgccaac tacccacaac ggcactttgg tcaaatgctt tggcgatctc ccagggaagt
    14041 cccttttctt tttgtcttgc ctgtacatct ctggccgtaa aatcaatacc ggtagtaatg
    14101 gcatcaacat agttcagggc gaaagactca tcgatatgct taccattctt tccgatgcgg
    14161 atgaccagtt cagtctcata gtgcatgtct gctgaaaaat caggcatgaa gaaagcattt
    14221 ccctgcagca gggcagtgtc aggcttcagg aacagaacag gagttgtggg aactgcgttg
    14281 ttcagttctt tggcatggtc cacatagttt cgggcaatac agacgatctt catagaaaag
    14341 gcgctgcaaa gttacccatt cagggtaaga aaacctcagg cattgttgaa gcggttcata
    14401 gctctgtcta tgccttccag tacaaaactt tctgctgctt ctgcggcttt gatgagttgc
    14461 tcttccacca gttttttttc ttcgtcggcc cacttgccca gtacataatg aacctgagct
    14521 ccttttggaa aatcattccc cacgccgaat ctcaatcggg ggtagtctgt tgtcatcagc
    14581 atttcctgga tattccggag cccgttgtgt ccaccatcgc ttcccctggt tttgagacgc
    14641 aattttccgg tatgcaggtt aatgtcgtct gtgatgacca gtagttgctc tttttcgagc
    14701 ttgtactcct tcatccagta caggatggcc ctgccactca gattcatata agtagaaggc
    14761 ttcagcagta tgatctttcg tcctttcagc cggcattcgc tgcgccagcc atagcgtttg
    14821 tcttcccagt ttccttccag cttctggcat aacaggtcca ccacatcaaa tccgatgtta
    14881 tggcgtgtgc ctgcatattc actgcccggg tttcccaggc cggcaatcag aaaggtgcgt
    14941 tcactcatga cacaaaaaaa ggccaaccct ccggctggcc ttgtaaagat atctaattcg
    15001 tgcactagct tgctgactta gcctcagcag cttcagcaga ttgtttgctg cgcatagacc
    15061 ttgggatggt acaggtaatg accggaatgg cctgagaacc catgatctcc aggttgtcca
    15121 ctttgatctc gccgatcctt acagatttac ccatctccag gtgggttaca tctacctcga
    15181 tcaggtcaac cagatcttta ggaaagcctt tcacttttac ctttcggatc ttctgtgaca
    15241 atttaccacc tgcctttacg cctgctgcca ggccttttgg ctggaggggg atctcacata
    15301 ccaccttctt gtcagagctc aggacgaaaa aatcaaggtg gagcggtttg tccgttaccg
    15361 gatggaactg catgtcctta atgagtgttt catactcctt gccgtcgagt ttcacattca
    15421 ccttaaagaa ctcaggtgtg tagatgatcc cacgcaaggc actctcggaa acctcgaagc
    15481 tgatgttgtt ctcgcggcca tagagggtgc agggtaccat accctgtttt ctgctggcgt
    15541 tggaggcagc tttaccggta gaggttctta ccttaccttc aatgttaact gtcttcattg
    15601 tttgttgttt aattattgat aaaaagcgaa ctgatggaac gatgctcatg cgtgttacga
    15661 atggcacgtg caaacagctt tgctgatgac agcactctga tcttttccga ttcacggcga
    15721 agcgggatgg tatcagttac caccagctct ttcagttgag atttctcaat cgtgttgtag
    15781 gcttcaccgg aaagcaccgg atgggtacag aaagcggaaa cgctcctggc acccttgtcg
    15841 atcatgatat tggcagcgtt ggccagggtg cgaccggtat ccacgatatc gtctatcatg
    15901 atcacatctt tgcctgttac gtcgcctatc acggtcattt cagatatttc gtttgccttc
    15961 ttccgtacct tgtcgcagat cacgagttcg gtattgaaat gcacggcgta atgtctgttg
    16021 cgtttggttg atcctacatc cggagaggcg aatatcacat ttttgagatc caggttctct
    16081 tcaatgtagg gtacatatat ggcggtagaa ctcaggtgat ctaccgggat gtcgaagaat
    16141 ccctggatct ggtcagcatg aagatccatg gttacgatcc tgttggcacc ggcagcagtg
    16201 agcagttcag ccaccagctt ggaaccaatg gaaaccctgg gtttgtcttt ccgatcctgg
    16261 cgggccatgc cgaaataagg tatgaccgca gtgatgtagg atgccgaagc acgcttggcc
    16321 gcatctatca tcaggagcag ttccatcagg ttatcactgg gaaagtaggt gctctgtatc
    16381 aggaatacat agttgccccg aacagattcc agaatattgg gctgaaattc accatcgctg
    16441 aaacgatcta tctgtatccg gctcagtggc tggccgtaat aatcggctat tttttccgcc
    16501 atatagcggg aagtagtgcc ggagaacagt ttgacatcgt tggtgaccat gcgcccaggg
    16561 ttggattcag gagtgcaaag atagcaaaac agccaataat atcaggggtt aaaaacgaag
    16621 aaaggcccgg aaattccgga cctttcgttg gccgaccagg ggtcgaacct ggactcttct
    16681 gaaccaaaat cagacgtgtt gccaattaca ccatcggcca aaaggagtgg caaagatata
    16741 tcgcctgcaa attatttaca acaacccatc acaataccct gtgaagatgc cggataattt
    16801 tctcccggaa tgcctcatat atctttccgg tagcagggcc gctgaagcct gtatgaaccg
    16861 cggtgatgcg gtggtcccga tccaggatca gggtggtagg ataggccagg atgcgactca
    16921 gggtggggaa agcggcaccg gcttcctttt tttctgcacc accacccagt agcatggtat
    16981 atcgaatgcc cagttttttc cgcaaacggt cgaagttctc tgctacttct ttaaacgctg
    17041 tttcccgctc aaaacccact gctatgattt ccaaaccctc cggctgaaag gaatcataaa
    17101 tggatgacag gaaggctgat tcatccatac agttggggca ccaggatgcc atgatctgga
    17161 caatgatgac cttgttacgg aagcattcgt cagtcagaga agttgcattg ccttgctgat
    17221 cagggaagca aaaggtaagc ggttctgatc cgtcaaatcc ggtcagcatg tccggatcat
    17281 ccaggacgaa agtatgatct ctgaccatgt tgaattgttc ctgccaatgg atccccgaac
    17341 agaaaatacc gttgcaaccg gtgccgtcaa attctccggt aaacaaaaag gcgtgagaac
    17401 cgtcgaaggc tgacagggca aattttgaac cgttcacctc tccttccaga aagcgataat
    17461 cgcccgtatt ggtcagaaag gtgccgctga ggtggtttcc ctcctgttca aacatcccaa
    17521 tggccatcgt tactcctccg ctgtcagaag taaatgtcac tttccatgtt ccgcctatat
    17581 ctgccgttgg ctcttccgga aattctgaaa acctgtgata cacatctttg tctgcccgga
    17641 atggaagtct gtatccggtt tttctggata gatcttccca gtaacctgac attgaattaa
    17701 acgaggtatc ggcttcagct atgatctcag aatggaatat atctgagcgg atggtgatat
    17761 gaccgctatc ggtgtaaatt tctgtggtgg taagctgttc ttcaccattg atgacagctg
    17821 ctatccatac cgttcctgac tgatagatct ccagttgaaa tggcagctca cctgcaggtg
    17881 tttccagggc agctctccag gtaccagcca gtttttggat gttgattgaa ttggaagcca
    17941 tgactgcaag catcaaatat acaccttagc acgaccgccg cttacctttg ttccatgtgg
    18001 ccatcagtgg aacccgaaaa gattctgcat ggaaaggctc agctggtggt ggatgttcgg
    18061 tcgccggcag agtttgctca tgctcatatt ccaggagcca tcagcctgcc cttgttcagc
    18121 aatgaagaac gtgctgtggt tggtaagatc tataaggaaa acggacacga tgcggctgtt
    18181 tccaaagggc tctcgatagc cggaccaaaa gcggcagatc tggtggacac cattcgcaga
    18241 ttggcaggcg ataaagaggt gctgctgcac tgctggcggg gcggtatgcg aagtgccagt
    18301 gtggccatgc tgctgcagct ggcaggcatc agagggcggg ttctgaaagg cggttataag
    18361 gcatacagac gtatggtgct gggagctttt gaaaaacctt tccgcattca gatactgggc
    18421 ggttataccg gaagcggaaa aacagctgtg ctccgtgaac tggcagctac cggccagcag
    18481 gtcattgacc tggaaggcct ggcaaaacat aaaggaagtg cctttggcaa cctggaggat
    18541 gcagcgcaac caggaactga gcagttcgag aatgatctgt ttgaacagct gcagacgata
    18601 gatcctgaaa aaacattgtg gctggaagat gaaagccatt ccattggcag ggtctttatt
    18661 ccggaagcat ggtggaggca gatgcggcag gcacccgtgc tctttttaca tattcctgtg
    18721 gaagcccgtg ccacatggct ggtgaaagat tatacagaag ctgcacccgg agtgcaggca
    18781 gttgaaaaag cgctggagaa gatacgcaag cgattgggcg ggcaacatta ccaggaagcg
    18841 ctggagttat tcagaaacgg gctattgaaa gaagctgcca ccaaagcgtt gtattattat
    18901 gacaaagctt atctgtacgg actctcctca agagcagaag ataccataac ttaccaggaa
    18961 gagccgatgg tggatccgtt gatgatatcg aaaaatataa ttgaaaaata tgcatacaaa
    19021 tttgaatgac attaaactga ccgcctattc acatggagcc ggttgcggat gtaagatcgc
    19081 accggcagta ttggatgaga tcctttcttt tgctggcaaa caggggcagt tcaacgctct
    19141 gctggtgggc aatgataccc gtgatgatgc tgcggtcatg gacattggaa atggcacggc
    19201 catcatctct acaactgatt tttttatgcc cattgtggac gatgccaggc agtttggcag
    19261 gatcgcatct gtaaacgcca tcagtgatgt ctatgctatg ggcggtaaac cacttcttgc
    19321 tattgccatt cttggctggc ctattgataa attgcctgcc gaagtggctg gtctggtgct
    19381 ggatggtgcc agagaagcct gcacagaagc gggcattccg ctggccggag gacacagcat
    19441 tgacagcccg gagccgatat tcggactggc ggtaactggt caggtatcac acgaacacct
    19501 gaagaagaac agcggcgcaa gtgccggggt gctgttgttt ctgaccaaac cgataggtgt
    19561 aggcattctg accaccgcac agaagaaggg caaactgcaa cctgaacacg ctgcacttgc
    19621 acctgctcag atgatgcagc tgaacagcat cggtgaagtg ctggggaagc tggactatgt
    19681 ggaagcgatg accgatgtga ccggtttcgg attgcttgga cacctcagtg agatgtgtca
    19741 gggtagtgac ctgagtgccg aagtttttct ggataaggta ccattcatag ataccgcaat
    19801 tcttgatcac tacctgagtg aaggttgtgt gccgggcgga accaacagaa atttcaagtc
    19861 atacggacat ctggtggatg gtttgaatga tcgttcccgt aacctgctgt gcgatccgca
    19921 aacgagtggc ggacttctgg tggctgtgca cccggaaggg gaagaggcat ttcatcaggt
    19981 aatggagcaa tatgacatga agttgacttc gctgggaagg ttgaccgaaa gatcttccca
    20041 tgtgatacat gtatattcgt gatcatgaaa tatccccata tttttctcct gttactgatg
    20101 gccggttgta ctaaggatga gccattactg gatttttata actcgactta cagcggaacg
    20161 gtaacatatc acagttcgac caccatcagc ggggtgacca ctactaatga cagtacctat
    20221 tccgaaacca tcatattgag tatgataggc gaagactcgt tgaatatcag tagtccctct
    20281 ttcggagaac gcaaggtcct gatgatcctt cattcaaccg aatatatcac ctacgggtct
    20341 gcctattcct tttcctatta catcggttcc gacttcataa aactggagga tcattccttt
    20401 tccgggggag ctggtggctt tggttcaacg gttgacagga gcctgagtgc ctatcgggac
    20461 tgaccagcct gaagaccgtt gcaggtcata ctaaaatccc gtcgttttgt ttattttgca
    20521 agcatgaccc aaactagtat gtctgcacct tccgtacccc aggtttcaca aatggccgct
    20581 aacctgatcg gctccgagat catcaaactg gcggctgata ttaacaaacg catccagcag
    20641 ggagaacaga tatacaattt caccattggc gattttgatc cgcaggtatt tcctataccc
    20701 gaagctctgg aagaaaagat catacaggcg tatcgcagca aacagaccaa ttacccggcc
    20761 gccaatggta tagaacctct tcggaaggcg gtcagccatt ttctggagaa agggcttgga
    20821 ttaacttatt cacctgacga tattctgatc agcggtgggg gaagaccatt gatctatgca
    20881 gcttatatgt ctgtagtgga tcccggagac aagatcatat atccgctccc ttcctggaac
    20941 aacaaccatt acacgcattt gagtggcggt gttccggtgg agatcgaaac cagtgcagcc
    21001 aatcatttca tgccaacggc cgatgatctg aaacctcata tcggtgatgc taccctcatc
    21061 gccctttgtt caccactgaa cccaacaggc acgacgtttt ctaaggaagc gctgaccagt
    21121 gtgtgtgagc tggtggtgga agaaaatgcg agaagagccg gtaagcaaaa gcctgtttat
    21181 ctgttgtatg accagttata ctggaccctg acctatggtg aaacgatgca tcatgatccg
    21241 gtcagcctgg tgccgggggt cagaccttat acgatttata ttgacggcct gtcgaaagcc
    21301 tttgccgcta ccggtgtgcg tgtgggctgg tcggcaggac caccgcatat catcgcaaag
    21361 atgcgggcca tactttcaca tatcggtgcg tggagcccca aacctgagca ggtggcagcg
    21421 gctgcatttc tggaagatga cgaggccgta cagagctatc tggaaacgta taaagcagca
    21481 cttcatcaaa gactggaagg attctacagg cactttcagc acctgaaggc aagcggtttc
    21541 ccggttgact gtatcgcacc acaggctgcg atctatctga cagtacgaat tgacctgcgt
    21601 ggtgccacaa cccaatcagg tgagcagctg accgacatgg cagcagttac ccgttactta
    21661 ttacaggatg ccggcctggc gatcgttcct ttctatgcgt ttggaacagg ccgggaatca
    21721 ccctggttcc ggctgagtgt aggcacctgc aggatggaag atgtggatgc atccatggag
    21781 aaactgggtg cagcactgcg cagtttgcag ttcagctgat cagtcgaaat agggcagcat
    21841 atcttctgtg atctccaccg ttttaccggt ggtgttgtcc accatggcaa aagtgatggt
    21901 ggcttcagct atgagtttat cgtctgactt tcttattacg gtttgtttaa cacgggcgct
    21961 cttgtttcca acttcgtctt tccttacgga taccagcaac acatcgttca gcacagcggg
    22021 tcttctgtaa tcgatgttga tattcaccac aacaaagctc attttgcgtc tggcaaaata
    22081 ctctgttgcg ccggtggcat tcacccagtc ccagcgacag gcttccaggt attccagata
    22141 acgtgcattg ttcacgtggg cgtatatgtc gcagtcaaaa ccacgaactg tgatctcagt
    22201 ggtatgcata gcaggtcttt ccttactttt aatgcaataa tacaatgatt atgtacgatc
    22261 agccgatgtt acgcgacaat gccctgaagg ggaagacctt tatagtgacc gggggcggaa
    22321 caggacttgg caggtccatg agtaaatatc ttatggaact tggtgccagt gtggtaatct
    22381 ccagccggaa actggatgtg cttcagaaaa ccgcagatga actccaggcc gaaacagggg
    22441 ggaaggcgct ggcagtggct tgcgatgtca ggatttatga ggaagtggag caaatgctgc
    22501 aacaggctgt tgatgccttc ggacaggtgg acggattgct gaacaatgca gcaggtaatt
    22561 tcatatcgcc gacagaacga ctgagtcacc gggcatttga cacagtggtt gatatcgtgc
    22621 tgaaaggttc atataactgc acgctggctt taggcaagta ttggatagaa aagaagatct
    22681 ccggtacggt actcagcatc accactacct acgcatttac cggtagcgga tacgtagtac
    22741 ccagtgcctg tggtaaggcg ggggtgatcg ccctgacccg gtcgctggct gtagagtggg
    22801 cgaaatacgg tatcaggctt aatgccattg caccgggacc ttttccaaca gaaggtgcct
    22861 ggagtcgcct ggtgcctccg ggactggaag atgatctgga catgaagaaa aaagtgccgg
    22921 tgggcagggt agggcatcac caggagctgg ccaacctggc agcctatctg atgtcagatt
    22981 tcagtgcata tgtgaatggt gaagtcattg tgatagacgg cggcgaatgg ctgaagggtg
    23041 ccggcgaaat gaatgtgctg gagaaagttc cggatcagtt gtgggatatg atggagaaga
    23101 tgcggaagaa atagtgagtg gtgagtggtg agtggtgaaa tctgggggaa caagttactg
    23161 cttgaataga acgaattctc agatcacatt gacttccggc gtgaggctga tctcgaatct
    23221 ttctttgatg ttgcgcatga tgcgctgggc gtgttcccag atctctatac cagtggcatt
    23281 gccataattt accagcacca atgcttgttt gctgtgtgca ccggtattac ccgtgcgtcg
    23341 gcctttccaa ccgcaggtct cgatcagcca tcctgccgga acttttacaa ggtcggcggc
    23401 agaacctggg taacctggta tgtccggata ctccatttgt aattgttcat aatgttttct
    23461 gctgatctcc ggattcttga agaatgatcc tgcattaccg agttctgccg ggtctggtaa
    23521 tttactttgc cggatgctga tgacggcctg acttacatct gcaatgttcg gttcagttat
    23581 tcccatggca tccagtgtat cctgaatggc accatatgaa agtctgagtt ccggacgagt
    23641 atccagcctc aaagtgacag aggtgatgag atatttaccg gccagttcgt gtttgaagat
    23701 gctgttgcga tacccgaaca tacaattggc cttgtcgaat gtatgcaatt caccggtttc
    23761 cagatcgagg gcttccaggt gatgaaatac atctttcagt tctaccccat aggcaccgat
    23821 attctgaatg ggtgcagcgc caaccgttcc gggtatcaga gagaggtttt ccaggcctcc
    23881 ccagcctttt tgcacagcat agagaaccac atcatgccac acttcaccac cggctgcagt
    23941 aagcagcaca aaacggtcgt tgtcaggatc tggttgtgca gatataccct tgatcctgtt
    24001 aagcacaatc aggcctggat agtctttcgt gaacaggata ttgcttccgc cgcccagaat
    24061 caggaatgta ggatactgtc ttagaagttc ctgaagtact tgcagatcat cctgttgctt
    24121 caccgtgaac agtaatgaag ctttgcagtc tatgccgaag gtatggagac cggccagtga
    24181 tgcgttatga cttggtaatt tgcttgtcaa gactgctgac acaatggtga tcaaatgtag
    24241 tcaggtattt tttagcttta ctgggagata tccaccgtct atgaggttat ttgtactgat
    24301 tctgacactg atcttcggca tttctgagct ttcagcacag gccattcccc tgatctcgcc
    24361 tgacagggat atgactttca ccaagcgcaa cacgctggat gaagacagtg cctatttctt
    24421 tatacgtacc acttctgtag cagacgaggc tgatggaaca gcatactatt tcaatgtgaa
    24481 catagataca gctggcggag gctgtgaaac ggacaccaca ttcatgggta gaaaggccct
    24541 gctgctggat gatatctatc agacctatgt tttcttcaat gatcaacagg acagtatatt
    24601 catcagaaca gatgtggaat tatatgattc atgggtattc tatacctggc cggatgggaa
    24661 atatatcaaa gcacaggcaa acaacaagat cttcaagccg atcatgccgg gtgtgaatga
    24721 tactattgtc cgtatcaaac tcagtgtgtt cgacccctcc aataatccgc tggacatttg
    24781 gcctgataac cagaagttcg atcttagtga acaatacgga cttgctgaac tgtacaattt
    24841 ctatgctttc ccgaatgatc aggagttgat cctgttaaga ggagtttcca atccggacac
    24901 agccatcacc gatgtgtctg ctcatgctgc tttggatata gcccctggct atgagatgca
    24961 ttatgaagaa ttctttgaag atgaaggtta ttttgatctg cacagacttt ataaattgtt
    25021 tgtacttgac actttgcata atgacctttc ggttgtcatt acaagtcagc gcacgctttg
    25081 gcaggaacta actttgtctg gcacaacaga tacactacaa gttctggaca ctatcacagt
    25141 tgaatatttc tactctgact tagcccagct ggatacacct gagcttcaat ggatgcagta
    25201 tgaggaggga gcatttgcat tcagtgactt tatactggaa ccggatacct attccgggat
    25261 ccgtcataaa tggctttatg gtggatattc atccacttca gaagatacat tatgtgcatc
    25321 gatcctgacc ggagcatcac cctggccgtt gaagttgtac ggaaatgaac taggtatcat
    25381 gtataagctt gattctgttc ttgagttact tgtacctgat gcaattgatc ctgactatgg
    25441 caatgtggaa ttacaaatgg tctatttcca gaaaggcctc atcacctggg gcgaacccat
    25501 agatttctat gagcttggtt ttaccggtat caatgaacca gcagagctgg cttttcagat
    25561 atatcctaat ccggccgaag accatttgca gataagtgga gttcaacagg cagcttatat
    25621 caatatatat tctgcagatg gtcgattgct gagatcagaa ccatattatt catatataag
    25681 catcagtgaa cttccttcgg gtatatacct gctgcagatc atgagcgatg aaggaactgg
    25741 aacgaccacc ttcatcaaac agtagcagtt ctatttgatc cggttcaatc ccgccttggc
    25801 ctggctgtcc aggctggtct catattcctt attgggcata ttgaggcagg cattgtaata
    25861 gtatgcagcc ttttctttgt tgccttcatg ttcatagatc aggcctaact gtaatgcagc
    25921 cgaggcagca taataatatg gcagctttcc accgttcttg atcgtagcag catagaaccc
    25981 tttggctttt tcagggttac cactctcatg gtagatacgg ccagcacgat aggtgaattc
    26041 agtcttgtct ttatcggatt tgaaattgtc ggtagataat ccttccagtt gtccgatggc
    26101 ggtcttgtag taaccaccat caaaaagtaa acgggctttt aacaaggtaa cgtttggtgg
    26161 tattccactt tcagcttcca gcagggcctg cttatcgtcg tctatcacat cgtcgccata
    26221 cagtttgacc cgttccatat agaacttata ccgcgacata tcgccggcaa tgagggcatt
    26281 ccatcccatc ttctggtagg cttccttgat gtaattcttt cctttgaact gaaccagaaa
    26341 actctcaaaa tatctgttgg catcgctgtc cagccggcgg gtcttggcaa gtcccagcat
    26401 gaaatccatg aaggggaagg ggaaataatt gtttccggtg gggcggctct gtaaggtgga
    26461 gatggcagca tcgcagcgac cggtgcgcag tgctacgctg gctttgcaga agcagaaaag
    26521 catgttgttg cgggtatcca gatccttgac aatgttccat gctttgttgt aatctttttg
    26581 cagatataat accagaaaag catagtacag ggtggtctcc tgaacaaata tttgggggaa
    26641 atcgccgggc ttatccagga aggaccgcat ttctttcagt ccggcatcaa tatcgccaga
    26701 caagcccaat atattggcgc cccatttata ctggtcaggt atggcgccga acagacagtg
    26761 cagcataccc aggcttttct ggttgggaag aaaatcagga aaatcttcgt cgttatcttt
    26821 cagcattccg taggcacgct tgatctcatt gaaggcgttg aggtattctt caaacttcag
    26881 tcgggcaaag gcccactgaa tattgacctc ggcctgcagg tacagatagt acggggagga
    26941 agcgtcgcct gacttcagct tacttaaata gatgtcttta tgcggttcca gttcatcaaa
    27001 acgagcctct tcttctgaga tatagatgga gatgatgtcc atcgtattct ctatataata
    27061 gggaaaaaga ttgcctggat tggctttccg ttcggccgct atgatctttt tcgcttcatc
    27121 gaaacgcagc gccatgacca tattcatagc atcctcacag cgttggttca cctcataggt
    27181 agccgctatg gaaaccaatg gccggcaaaa tacggccaac aataaaagta ataggaatct
    27241 ggatctcatg ggcccgtggt atcgcaaaga tagcgggcaa atatcgttcc tgcttagtct
    27301 tctgaaatgc tgtcatccac cagaatgcga ccacaatgtt cgcaaatgat gatcttcttg
    27361 gcctgcttga tctcgctctg gcgctgaggg ggtacttcag cgtaacatcc gccacaggca
    27421 tttctttcaa ctgttactac agcaagtccg tttctgtaat tgttgcgtat gtgataatag
    27481 gctgccagca ggcgttcttc cagacctttt tcggcagctg caactttttt ctcaagctct
    27541 ttttcttctt tctcggtatc cttctgaata tgttccagct cttccttctt cttagccagg
    27601 tcttctttct tgtgctctat attgatcttg ctggtatcaa tggcttcttt gcgggcatcc
    27661 ttgctggcag taagctcctt gatcttcttt tcgctcagct ctatatccag cttattcatt
    27721 tcgacttctt tggtcagtgc gtcgaattca cggctgttct tgacattatc ctgctgcttc
    27781 tcgtacttct tgatattggc cttgccatca gtgatggcat ttttgcgttc agcaatctgg
    27841 tcatctactt ccttaagctc tgcttccagc ttgtggatac gtgtttcaag tccggctact
    27901 tcatcttcca gatcacttac ttccatagga agctcaccct ttaggatatt gatctcatcg
    27961 atctttgtat ggatcttttg caggttatac agatgttgca gactttcttt gatcgtcagt
    28021 tctttcacag tggccatgtt cagaaatatt taaccggatt tgtatccatt ccctttacga
    28081 tcgggaatga aggcgcaaaa ttagcaaatt tttgattgag aaagcctatg atcaggtcgc
    28141 tggtgaactg ttcactctca aaatggccca tgtcggccag aattataaca tcctcagcat
    28201 caaagaattg gtgatatttc aggtcggcgg ttacaaaaca atcggccccg gtcctgatgg
    28261 catcgggaag caggaaccct ccggctccgc cgcaaacggc caccttcttt atgctgcggt
    28321 gagtggatcc tgcatacctg atcacgggtg tttgcatctg gtctttcagg tattggagga
    28381 aggaattcac atccatcggc tctgccagtt cacccaccag tccggcacct gtttcctgca
    28441 gggcattgtc aagcggatat atgtcataag ccacctcctc ataaggatga gcttcccgca
    28501 tactattaag aatagctgct tctttccatt gttcaaagat gacctctata cgtacctcag
    28561 cttccaggtg gtcttcgcct ttaattccta caaaaggact ggcagattct cctgcacgga
    28621 aagttccttc tcctgcggta ttaaaactgc aatgatcata ggctccaata tcaccggctc
    28681 ctgcagcaaa gagtgcaaac ctgacttctg ctgcatgcgc ttcgggtgcg aatgtgacga
    28741 gttttttgag caatcctttt ataggacgaa gcaccctcgt ttcctgcaat tgcagtttcg
    28801 cggcaatggc ttgattcacc ccacctctaa cattgtccag attggtatgg atggca
//
LOCUS       DAOMAX010000006        27012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_186_length_27012_cov_4.405238, whole genome shotgun sequence.
ACCESSION   DAOMAX010000006 DAOMAX010000000
VERSION     DAOMAX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 27012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27012
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186_length_27012_cov_4.405238"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PK742_00935"
     CDS             <1..658
                     /locus_tag="PK742_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQU75181.1"
                     /translation="YRPEVQLLASRGYAVMQVNFRGSWGFGKRFTEAGFREWGRKMQD
                     DVSDGVQWLIDNGIADPDKVAIYGASYGGYAALAGLTFTPDLYACGISYVGPSNLFTL
                     LDNLPAYWEPEKEMMYETIGHPKQDSSLLCKVSPVFHVSSITAPLFIAQGANDVRVTK
                     VESEQMVEALRSKGVDVVYMLKEDEGHGFRLEENRLEFYQALCGFLETYMPASPPDKP
                     "
     gene            complement(625..1785)
                     /gene="hemW"
                     /locus_tag="PK742_00940"
     CDS             complement(625..1785)
                     /gene="hemW"
                     /locus_tag="PK742_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQU75182.1"
                     /translation="MSGIYIHIPFCRQACHYCNFHFSVNRRLLPEMVEAIQTEMDRKL
                     GVQNRPATETIYLGGGTPSLLSYRQLTDLIDRIRKHTRVVPDAEITLEANPEDITPAH
                     LQEWRNAGINRLSIGIQSFNDNDLLRLNRNHTGVMAEEAVLSAASFGFDNISIDLIYG
                     IQGSGPDDWKKNIDKALLLPVQHISAYALTVEPKTAMEVRIRRGQDEHPSETAMVEQF
                     YLLLQMLEDGGYEQYETSNFCRPGHISRHNTSYWEGKPYLGFGPSAHSYDPPVRSWNL
                     SNNTLYIQQVRAQMPEAASEVLTMEQQANEYIMLQLRMRKGLDLSAFRLRFGSPLYDQ
                     LQQNAVHSIQTGQLVHSGNILCISKPSLMLADGIIAGLFVEDQGLSGGEAGI"
     gene            1826..3583
                     /locus_tag="PK742_00945"
     CDS             1826..3583
                     /locus_tag="PK742_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQU75183.1"
                     /translation="MRLKLVILCIGLGWAYTLMAQVYTIENLHELDLPPSLMKQQGKG
                     DVRWVENVVQHMREDGFLLSGSETIIAGQDSVTLRIIPGKRYTWAHLSTDSIDTQLLS
                     AAGLKKFDPDGAPVSYQEIAAAAGQLLTYAEEHGFPFATVELCRLNLEDTVLSASLCL
                     QLNRFIRFDTLMVLGNSLLSAHYLEIYTGIQPGQPYQESLVRDLDDRLDELSFVRRTQ
                     PSVLRFSGNQAKAVMYLDVRNASKFDLIVGFLPNNEITGRLIVTGQGTLDLRNVFGQG
                     ERLGIQFSKLESTTKSLQTQFSYPYLPALPLGIDASFDLFLKDSTFLERNADVGVLYT
                     LGADHYLKALVRWYNSDVLTYDSLQVLTSKSLPAQLDVQLRSYGLEWGLSRLDYSPNP
                     RKGWMFSVTGMAGTRTIRRNASILQLEDPADPSFDFATLYDSLDERSLTVQYAYDLQY
                     VWPLSGSFVWMMRARGASQINPAVYANELYRIGGNQLLRGFDEQSVLASDYHILTLEP
                     RYLLSRNAYAGVFTDVGWIANRSLSPVVNDLPVGFGAIFTFETKAGVFGLTYALGTRQ
                     DAPVLVRNAKIHFGYINYF"
     gene            3614..4387
                     /locus_tag="PK742_00950"
     CDS             3614..4387
                     /locus_tag="PK742_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HQU75184.1"
                     /translation="MVRVAGLQANDQVIMPADSAWGMQPSDGTVYAQPVRTSSEEQGE
                     VVILLLLLPVFSLFVFAFRPYLSTNLLVSGRMQLADQIMREREFSSGLPGLVLHVLFS
                     VSVGVLLWHLFPEAVASLLPSGHTWQLLILFTVWPLLYWFRFLVSQVFGLIFRMRSAI
                     GRFRFRADILIETTGFFLFPVVCLLMLSPSAMQEIWLAASAIILSILVIWRWYALLRV
                     AGSLAGFRVSYFLLYFCAFEIAPVLVVFALVSGRSGLDF"
     gene            4398..5186
                     /locus_tag="PK742_00955"
     CDS             4398..5186
                     /locus_tag="PK742_00955"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQU75185.1"
                     /translation="MAQQSADLKPVKTILVSQPQPENPKNPYADIEAKFKVKIDFRKF
                     ISVEGVPAKEFRKQRIDINEHTAIILTSRNAIDHFFRICEELRIRMPESTKYFCQSEA
                     IAVYLQKYIQFRKRKVFYGPNGKDKELTDLLAKHISKEKFLLPCATLHQDAISAFLEE
                     KKANFSEAIMYEARFADLSDMKGSIMHDMIILFTPKGVESLFQNFPEFKQGDTRICGM
                     GAGTQKSMEDHGLRVDIKAPTPESPSIFMAIENYLEQSNRRKKK"
     gene            5231..7255
                     /locus_tag="PK742_00960"
     CDS             5231..7255
                     /locus_tag="PK742_00960"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQU75186.1"
                     /translation="MHPLRSDHTNTLVHATSPYLLQHAGNPVHWQQYGPDAFELARQE
                     DKPLLISIGYAACHWCHVMEHESFENQKVADFMNEKFICVKVDREELPHVDHYYMSAC
                     QVISGQGGWPLNMFVTADGKPFTGGTYYPPKPGYGRPSWMDVLAFVSDIWKNRREEVL
                     EQADKLSAHVHQSQASLLTLKPESVADGSDTEKIFRHLMSQADEVNGGWGGAPKFPAA
                     MVLQFLIREEWFRPDDQVRKHVQKTLRHMHYGGIWDQLGGGFCRYTVDASWLIPHFEK
                     MLYDNALLLETYALAYGRWQDPEDLEVLNGILAFANRELVAPGGGYFASLDADAEGEE
                     GRYYVWTNEEIESLLADDAVWFKKRFQVSEDGNWEGRNILHRNHPGGKSPSSPGQEAA
                     MAALLQARSERVKPALDDKILVSWNGLFLHAMLVAYRATRRADLLQATLDHTKALLEH
                     CHVDGILYHVWCKGQVTVPANLDDHAALIRLLLALFGETGNKYWYDQALSLAEFAVGH
                     FSDPESPLFYDHLPMESLPVRTREVFDYAQPSGNALFAQSAFTLYLYSGEQRWLDLHH
                     QLTSAMQTGMERFPQACGQWLQAAQPLQHPVAELVTAGPEARSLLAECHAEWYPLLIS
                     LPVCDRSLENMPFSTDRFDDNVTRIFLCKDHQCAAPVSTVSEYAKLLKAF"
     gene            7333..8310
                     /locus_tag="PK742_00965"
     CDS             7333..8310
                     /locus_tag="PK742_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU75187.1"
                     /translation="MKSRILISAILLLCSMFRASAQDEPQFSQYMAAPVLFNPAAAGL
                     DQAWNSALLVRNQWVGLPGAPTTQAIATELPVYRIRSGISLRVMNDRAGQQNTTVANI
                     GYSYQLRAGSGLLGLGVHAGIASRSLDGSRLIAPDGSYEAGVDHNDDQLPVTLVSSLI
                     PDAGAGVFFRNERLQAGLSVTHLAGSSFSFDAPGGSAGIQYHPVGYVFVSWKVLQQEN
                     WSLEPGVLYKTDLTEQMLDANLLFSWRETLLAGLSLRSLDAGRRDALVAMGGWNFSKY
                     WGIRYSYDLTLSALNNAQSGSHELVLTYRYPLAPPRAGKEINNLRYLYY"
     gene            8453..9745
                     /locus_tag="PK742_00970"
     CDS             8453..9745
                     /locus_tag="PK742_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQU75188.1"
                     /translation="MKRKLSFIPALFAGSVIFLSGCVSSDWDDYHGQLLGMQDRPVWE
                     PINPYGMVYVPSGTLHIGPSDQDVNNSLWQRQKSISIQGFYMDDTEITNNEYRQFVFW
                     VRDSIAHVLLGHYAEQEEGYQTEVPVLDWDEEIDWEDDEQRPKLQEMLLPPGEYTYYR
                     SVDPNRLVYTYQWIKWKDAASKELNKDREKYISEFKDEKTVKVYPDTLVWVHDFTYSY
                     NEPMTRQYFSHPAFDDYPVVGVSWHQANAFSHWRTQFWNWWRIKHDEIIVDDFRLPTE
                     HEWEYAARGGRDLAPYPWGGPYVRNTKGCFLANFKPGRGNYPEDGGYYTVNVYSYAPN
                     DFGLYNMSGNVAEWTSTAFRENAYAFIHDENPDIRYDAEDSDPPAMKRKVIRGGSWKD
                     ISYYIQTGSRHWEFQDTAKSYVGFRCVMPFLGRSMSDY"
     gene            9839..10666
                     /gene="gldL"
                     /locus_tag="PK742_00975"
     CDS             9839..10666
                     /gene="gldL"
                     /locus_tag="PK742_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HQU75189.1"
                     /translation="MSAFFESDRFKRIKNLIIGVGASVVLVGALFKILHWEGADIMLM
                     VGMFTEAFIFLLLGVLPPHKDYYWEKIYPDLDIAPEVEEAKTGKKLNKLEPKKSVTQQ
                     LDNMLEEAQVEPLMIERLGENLRKLGENIEKLSDVQDAAVSTNEYSSKVKEATAAVTD
                     MREAYNSAAAAMSQITASSVDTTKYHEQVQMVTKNLSQLNAIYELELQDTNNHLKAMN
                     RFYGSLNSAMDNLNESLTDTNKYREEISKLSGNLASLNRIYGNMLNAMNMGAGNTAN"
     gene            10711..12324
                     /gene="gldM"
                     /locus_tag="PK742_00980"
     CDS             10711..12324
                     /gene="gldM"
                     /locus_tag="PK742_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HQU75190.1"
                     /translation="MSIPKEPRQLMINLMYLVLTALLALNVSAEILNAFNLVNKGIGN
                     TNTILQDKNNQIVAGIAGKADEGDDPRAKDIAKDAAGIQKVTADFYAYVEMVKDSLIS
                     YTGGMIEDKHHPGQEKLKGESDTERPTTLLINKGLATELKTKIEETRQEYVKLLQKWN
                     GEGKVNQLTLNVEDGGGEQGLSWEESNFYKVPAVAAVTILTKIQNDAKSAESTVLEHM
                     ANQIDAAKIKFNKMTAMVTAPTSYVKRGNEYTADIFIAASSDQAQIEVYTGSFTAAVK
                     KDEFDQFIELEGSAPPLNNPQKIDVVGGMGKIKETAGGQRNFQGVISIPDPVKPGNFK
                     FYPFEFGYETFEVGEAVVSPTAMNVLYIGVDNPIKISVPGYTSDKVTASGCGISKVKG
                     EEYVARPSNVGKENINVTVNTGEGTKSHSAEFRIRRIPDPYPYCGTSKGGTMRIGEFK
                     QVSTIVAKNPDFVFDINYQVTGFEMVYAPRSGGNVVSDVSNNAKFSKLMDDIKTKAKP
                     GDTIVFPRISVKMPDGTTRQVSLSFKLIG"
     gene            12338..13174
                     /gene="gldN"
                     /locus_tag="PK742_00985"
     CDS             12338..13174
                     /gene="gldN"
                     /locus_tag="PK742_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HQU75191.1"
                     /translation="MKTVIRSVFALVVIFTATAAMAQPTSEAKRDRAYDRDLLPQREV
                     IPYDHIREADVFWEKRVWRQLDFREKMNLPFTWPVDPFMDFIYKAIMNGELTAYSPLY
                     DDFGEGTALTIEDIKNKYEGVDTTYTYDDETYEEKILLIPRQFDYQNIKKMQIKEDWI
                     FDEETSTLVVRIMGMAPIIERIDPFTGEPIGDEIMFWLYYPDLRNILVRHEVFNEKNS
                     ARYITFEDIFEMRYFSSYIIKEDNVYDRFINSYAAGIDLVLESDRIKNEIFEFEHQLW
                     EF"
     gene            complement(13221..15020)
                     /gene="uvrC"
                     /locus_tag="PK742_00990"
     CDS             complement(13221..15020)
                     /gene="uvrC"
                     /locus_tag="PK742_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQU75192.1"
                     /translation="MTPEAFKELETTLPAKPGVYRYYDADGNLLYVGKAKHLRKRVSS
                     YFQKSDHAARIKLLVRKIDRIEFTITNSEQDALLLENTLIKQYQPKYNVNLKDGKTYP
                     YIVVKNERFPRIFFTRNRKDDGSTYLGPYPSLGRVRTVFEFIQSLYPMRTCSLALSEA
                     NIKAGKFKVCLEYHLGNCKGPCEGLQSEEDYMANVKEVIRILKGHMGELKTELRSRMH
                     EYTEALAFEQAEDIRRKLEAIDAFQGRSTVVNVRMDEVDVFGYTENEKEAVIAYLQVN
                     EGMIIRSKTVVLQRKLDESREELLAYAVNHLRREMQSSCKEVIIPFDCSPDDDGLIKT
                     IPLAGDKKKLLDLAFKNALYLREERQAGKQKREDKNANLRILDTLKEDFRLQERPHHI
                     ECFDNSNIQGSSPVASCVVFRNARPSKKDYRHFNIKTVVGPDDFSSMKEIVYRRYKRL
                     TEEGESLPQLIVIDGGKGQLGAAVESLKELGIYGQVAICSIAKRLEEIYFPEDPLPLY
                     INKKSESLRVIQQLRDEAHRFAITFHRQQRSKKSIRSGLDGIPGIGPASQKLLLKTFK
                     SVKKIREAGEETVAKEIGPAKARALFAYWESEQ"
     gene            complement(15069..17360)
                     /locus_tag="PK742_00995"
     CDS             complement(15069..17360)
                     /locus_tag="PK742_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQU75193.1"
                     /translation="MSIREEFKQYRTYLRWFWFTVAGGILAFTLFIVLLSFGVFGALP
                     GFEQLENPQNSLATQVMSSDGELLGRYYKENRTNITFENLPPYLVQGLVATEDVRFYD
                     HSGIDGRSLARVLFKTIILRNENAGGGSTITQQLAKNLYGRPTSSSIFSLGLIKFKEW
                     ITSAKLERRYTKEEIITMYFNTVEYVYDAYGIQTASKTFFNKDVDSLLIEEAATLVGM
                     VNNPSYYNPVRYPDRTRDRRNVVLYQMKEAGYLDVAAYDSIKELPLDITRFKRQDHTS
                     GEATYFREYLRLWLADWAENNPKPDGTNWNIYRDGLKVYTTIDSRMQLYAEEASAEWL
                     TKLQDEFNKHWEGNKYDPWSYPYRGRTQPGLIDQAMKQSERWRVGKKAGKSEKELEEE
                     FHTPVETTLFNWTAPNRELDTLISPYDSIRYVKKTLHPGFMAVDPASGQIKAWVGGVD
                     MRFFQLDHVNSNTKRQVGSTFKPFVYTAAIADKGFSPCQEVPNLPVTFEKGSRWGLQK
                     DWTPKNSDGKYGDMLTLSMGLGKSVNTITAYLLREVGIQPVIDLAHNMGIESEMPFQP
                     SICLGVSDISVFEMTSAYTAFVNKGVAVRPVFITRIEDKNGNIIAEFPTQRVEVMREE
                     IASVMLNMLEYVVNYGTGNRLRRDAYGGIRGEVAGKTGTTQENTDGWFMGVVPQLVCG
                     TWVGADDPVVHFRSTALGQGANMALPIYGLFMKRVYSDPTLGIDPNAKFQRYEGPQTI
                     ELDCSKYKTSQQDVYEDLLEGYN"
     gene            complement(17357..19870)
                     /locus_tag="PK742_01000"
     CDS             complement(17357..19870)
                     /locus_tag="PK742_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU75194.1"
                     /translation="MYHDLTARDNGYFNAKLLLSQSRQTLWDGQTEDFSALLPVYKFG
                     DKESAQGEQSNLDEVIKKSSIVIQLHKKSKWVDDCYMLIGQANFYKKEYDEAITSFQY
                     IISKYSDQGKRKKKKKKKSADEESWLKEHLSHHPVANEAALWVVLTLTEKGAYTEAKT
                     ALSVIKGNPKFPSQLDDELAAVEASLYLKQRQYGAAIKALQTAEELTKDKALKARYAF
                     ILSQLYADAGEFSLSQEGFQRVIKYKPDYVTDFYARLFTATQGMQQSGTASSAAVSTL
                     TAMLKEDKYKEFYPLIYNTLAEIELRSSNVEDAVNYLKEGIRTGDVNPAEKALSYVKL
                     GDIYYEQTEYKPAYNQYDSALQTLDRAFARYKEIETRRDGLIDLVKALTTIETEKKLQ
                     YLASLNEFDLDKELDKMIAEQEAANQQEFLDGAAAGDDRNEGNTGEFYFYNATLLSRG
                     FGEFKKVWGNRSLEDNWRRSDKRSFDEETIGENENQDATEESEIDLNSEQLTKEDIIK
                     SLPRTPELLEASNRRIAGALYAAGNIYRQKFENDNKAVEYFRENINDFPDNPFELQSL
                     YQLFVIFDGQPAQDQYKSSILNKYPESLFANIIRDPDYLEKQLKQNEQLEDYYTTTYD
                     FYTAGDLSTVRMRLTAADSLFPNNPLQPKFDMLEALSLSDTASIGTFAAALQSIVDKY
                     PTDEVGIRAKAILDYINKTEAKEEAIDPSELYSYNSEEEHYVILVIPSKGKEATSIKN
                     ALADFNTTNYNVRKLRVSSLLFGPEQTLILIKTFTDASDAMDYFSFVENEYEEIFEDI
                     DMNDTFFFVVSKSNYVQLYKSKEAETYIGFFEENYLTEE"
     gene            20066..20944
                     /locus_tag="PK742_01005"
     CDS             20066..20944
                     /locus_tag="PK742_01005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQU75195.1"
                     /translation="MSTPSGEKQNWRQRLQNKYRLVIMHDKTFEVETSVKLSMLNLIV
                     LGSTLLVVMGVLVYTVIAVTPLKNYVSGYSEVSTTRQLLENSEIADSLMREMEARDAY
                     LDIITRTLKGELDTVVNKPDSAAIPQEQLNIDTISDIDRALRQQVEQEDLFNLTGADP
                     RQTGKENFRALYFFPPVKGTVTSSFDPSQGHYGTDIVAKEGTPIKTCLDGTVVDSYWS
                     VESGNVIVIQHSYGLISMYKHNSQVLRKAGSPVRAGDVIAIIGNTGELSSGPHLHFEL
                     WHNKVAIDAEELIIFN"
     gene            20956..21387
                     /locus_tag="PK742_01010"
     CDS             20956..21387
                     /locus_tag="PK742_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQU75196.1"
                     /translation="MFGNKQSADSTGNPGKGMLVSNRINHGTELTGELVSDGDIRVEG
                     VIRGTIKCKAKVAIGPSGLVEGDIFCKSADVEGRVKGDVEVADTLILKATSSVDGNIQ
                     TGKIVVESGARFNGICNMGQREKKVHVEKPVARTLQTEAVS"
     gene            21338..21577
                     /locus_tag="PK742_01015"
     CDS             21338..21577
                     /locus_tag="PK742_01015"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQU75197.1"
                     /translation="MSKSPSPEPSRPKRSVDQYLRYSNIAFQLFAAIFIGVWGGMKLD
                     AWLHTDPWFKVILSLLGVSAGLYSVLKEFIQPGKK"
     gene            21574..21960
                     /locus_tag="PK742_01020"
     CDS             21574..21960
                     /locus_tag="PK742_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75198.1"
                     /translation="MKKFLITYLVFTFLVACALWGILQIPVLHPASSFAWICFAVFFI
                     LTLLTFWMAARNMEGRFQQFMNIFFGGIIIKLFVTGAMVLIYKSVAGAPEQSFVLIAP
                     FALIYFSFLGFETSQLLRLNRKQTGS"
     gene            complement(22065..22138)
                     /locus_tag="PK742_01025"
     tRNA            complement(22065..22138)
                     /locus_tag="PK742_01025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22102..22104),aa:Arg,seq:tct)
     gene            complement(22174..24333)
                     /locus_tag="PK742_01030"
     CDS             complement(22174..24333)
                     /locus_tag="PK742_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQU75199.1"
                     /translation="MKRFWIIVPVLLLVAACKESVQTTDIQGPDKVSYPQTATIDQND
                     DYFGTNISDPYRWLENEGDSAVRNWITAENDVTFGYLDQIPYRNQLEQRLTELANYPK
                     MSTPFHVGEYYFYAKNDGLQNQYVTFYKKGMDGEEQVFFDPNTLSDDGTVTLSMAGFS
                     ADRKYVTLSQSKAGSDWTELYVREIATNTQLDDKIEWSKFGGAAWYGDGFFYSRFDEP
                     GKNQTYTGASDKQKVYYHKLGTQQSADELVYSDPSHPKRYFFSSTTDDEHFLFVYMSE
                     GTSGTGILWRDLTSNDKKLRTLFEGFDWEYSVLDNEGDQLLVLTNYMAPNYKVVKVDP
                     KNPAPENWTEVIPEKEDLLEWVNASAGKLFAGYLKDVATRVVQFDRTGKQEREVQLPG
                     LGTAGGFSGEKTDSNVFYNFTSFTTPSDIYSYDVNTGKSTLYKKSEVQFDASAYETKQ
                     VFYPSKDGTMIPMFLTYKKGLKLDGTNPCLLYAYGGFNIAIKPSFSTSRIALLEQGFV
                     YAVANLRGGSEYGEEWHKAGMLEKKQNVFDDFIAAAEYLIGEKYTSSEKLAIQGRSNG
                     GLLVGAAMTQRPDLFGVALPGVGVLDMLRYHKFTVGWGWAVEYGSSDDSTQFQYLIKY
                     SPLHNVKPGTSYPATLITTADHDDRVVPGHSFKFAATLQAAQAGDNPVLIRIDTDAGH
                     GAGKPLAKIIEEDADWMSFTMWNLGLREFQQRESRKP"
     gene            complement(24395..26275)
                     /locus_tag="PK742_01035"
     CDS             complement(24395..26275)
                     /locus_tag="PK742_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQU75200.1"
                     /translation="MGDQSSHIHTSGHNQMAFVNALIHDLEALELMIRDHALENDVQR
                     IGAEQEMCLVNNEWRPDNNNLEVLAGLQDSCFTTELAKYNIELNCDPLDLQAGCFHRM
                     LEGLQLKYIKAQKAASVLHSRVIMTGILPTISRHEVTLDYLTPLPRYHLLNKQLRRKR
                     GKQFDLFMKGVDEIHIRHDNIMFEACNTSFQLHLQVAPEDFVQAYNWSQVIAAPVLAA
                     ATNSPLLFGKELWSEIRIALFQQSIETRKSVEELRDQRPRVTFGKDWIRNSITDIYKD
                     DITHFDVLITFDDPPDSLQEYRNGHIPELRALRLHNGTVYRWNRPCYGVYRGKPHLRI
                     ENRYLPAGPSIRDEVANMAFWTGLMLAKPAAPVSETHSFRDIKSNFFKAARAGIETVL
                     IWQGKELNTRTLILEELLPLAAKGLMSAGIPQEEISYHLNTIERRLKKQTGSQWQVRN
                     FRTLRKQLSPSDAAIALTAAIYENQVQQLPVAEWKDVHLDKANKYLIRHFTTVNQLMS
                     SDLITVFPDDTLLFVKKVMEWHSINHMLIENRRGELVGLITSGKIAELEAAGINPETN
                     SVRTYMKRTIQTIRQDALIEDALTIMRTQKITSLPVVRDNRLVGIITRNDMTRWLSFS
                     ER"
     gene            26352..>27012
                     /gene="ung"
                     /locus_tag="PK742_01040"
     CDS             26352..>27012
                     /gene="ung"
                     /locus_tag="PK742_01040"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQU75201.1"
                     /translation="MAREDIQLEAGWKAVLADEFDKAYFQQIRYLLLAAKRDGKVIFP
                     PGSQIFNAYNLTPFEQVKVVILGQDPYHGPGQAHGLCFSVPEGVKPPPSLVNIFKEQE
                     KDLGIPRPAHGNLETWARQGVFLLNAMLTVESGKPGSHQQIGWQYFTDATIKALSENK
                     EHLVFLLWGAFAQKKAELIDAGKHLILQAAHPSPFSADRGFFGCRHFSKANTYLQEHG
                     MQ"
BASE COUNT         6607 a         6307 c         6770 g         7328 t
ORIGIN      
        1 ataccgtccg gaagtgcaat tgctggcaag ccgtggatat gcggtcatgc aggttaactt
       61 ccgtggctcc tggggtttcg gtaagcgttt taccgaagcg ggcttcagag agtggggaag
      121 gaagatgcag gatgatgtgt cagatggggt gcagtggctt attgataacg gtattgctga
      181 tcctgataaa gtggccattt atggtgcttc ctatggtggt tatgcggccc tggcaggact
      241 gactttcact cctgatctgt atgcttgcgg aataagctat gtcgggcctt ccaacctgtt
      301 tacccttctg gataatttgc ctgcatattg ggagcctgag aaagaaatga tgtatgaaac
      361 tattggccat cctaagcaag actcttcgtt gctttgcaaa gtgtcgccgg tgtttcatgt
      421 ctcttccata actgcaccat tgttcattgc gcagggagct aacgatgtcc gggttacgaa
      481 ggttgaatca gaacaaatgg tagaagctct tcgcagtaaa ggtgtcgatg tggtctatat
      541 gctcaaggag gatgaaggtc atggattcag gctggaagaa aaccggcttg aattctatca
      601 ggcactgtgt ggttttctgg aaacttatat gccggcctct ccgccagata agccctgatc
      661 ttccacaaat aagccggcaa ttattccatc ggccagcatc agtgaaggtt tgctgataca
      721 gaggatgttt cctgaatgaa ccagttgacc ggtttgtata gaatgaacag cattctgctg
      781 caactgatcg tacaacggcg atccgaaccg gagtctgaat gctgacagat ccagtccttt
      841 tctcatacga agctgcagca ttatatattc gttggcctgt tgctccatag tcagtacttc
      901 agacgctgct tccggcatct gcgccctgac ctgttgaata taaagggtat tgttagacag
      961 attccagctt cttaccggag ggtcatagga atgtgctgac ggtccgaatc ccaggtatgg
     1021 tttgccctcc cagtaggaag tgttgtgccg tgagatatgg ccgggcctgc agaaattgga
     1081 tgtctcatat tgctcatagc caccgtcttc caacatttgg agcagcaggt aaaattgctc
     1141 taccatggct gtttcagatg gatgctcatc ctgcccgcga cgaattctca cttccatagc
     1201 agttttcggt tcaactgtta gtgcataggc actgatgtgt tgcacaggaa gcagcaaggc
     1261 cttgtcaata tttttcttcc agtcatccgg tccggacccc tgtatgccat agatcaggtc
     1321 tatactgatg ttgtcaaaac cgaaagatgc tgctgataga acagcttctt cggccataac
     1381 ccctgtatgg tttctgttca acctcagtag gtcgttgtcg ttgaatgact gaatgccaat
     1441 actcagacga ttgatacccg cattgcgcca ttcctgcaag tgcgcaggtg ttatgtcttc
     1501 cggattggct tccagtgtga tctctgcatc tggcacaacc cgggtatgct ttctgatcct
     1561 atcaataaga tctgtcaact gcctgtatga caacaacgaa ggagtgcctc cgcccaggta
     1621 tatcgtttct gttgcaggcc ggttttgaac gcccagcttc ctgtccattt ccgtctgtat
     1681 ggcttccacc atttccggca acaggcgccg gttcacactg aaatggaaat tgcaataatg
     1741 acaggcctgt ctgcaaaagg gaatatgtat atagatacct gacatgtggc gttatcaatg
     1801 aacgctctga ttacctttga ataccttgcg gctaaagtta gtcatattgt gcatcggtct
     1861 ggggtgggcc tataccctga tggcacaggt ctatacaatt gagaacctgc atgagcttga
     1921 tctgccccca agcctgatga aacaacaggg aaagggagat gtcaggtggg tggaaaatgt
     1981 tgtgcaacac atgcgtgaag atgggttctt actgtctggc agcgaaacca tcattgccgg
     2041 acaggattcg gttaccttac gtatcatacc cggaaaaaga tacacctggg cacacctgtc
     2101 aactgacagc atcgacacac aactattgtc tgctgccgga ttgaaaaagt tcgaccctga
     2161 cggcgctcct gtaagttacc aggagattgc agctgctgcc ggtcagttgc ttacctacgc
     2221 agaagaacac ggttttccgt ttgccacagt agaactctgc aggttaaatc tggaagatac
     2281 cgtactgagc gcatcgcttt gtttgcagtt gaaccggttc atacgttttg atacactgat
     2341 ggtattgggt aacagtctgc tgtcagccca ctacctggag atctacactg gaattcagcc
     2401 cggtcagcct tatcaggaat ctctggttcg tgatctggat gaccgcctgg atgaactgtc
     2461 atttgttagg cgcacccagc cttcagtttt aaggttctcc ggtaaccagg ccaaagctgt
     2521 catgtatctg gatgtacgta atgccagcaa attcgatctt attgtaggct tcctgcctaa
     2581 taacgagatt acgggaaggc tgatcgtaac tggtcaggga accctggatc tgcgcaatgt
     2641 tttcgggcag ggcgaacggc tcggcattca gttcagtaag ctggaaagca caaccaaatc
     2701 tttgcagacc caattcagct acccgtatct tcctgctttg ccgcttggca tagacgcttc
     2761 atttgatctg tttctgaaag attccacctt tctggaacgc aatgcagatg taggtgttct
     2821 gtacaccctt ggcgcagatc actacctgaa agctcttgtc agatggtata attctgatgt
     2881 gctgacatat gattccctgc aggttctgac cagtaagtca cttcccgctc aactggatgt
     2941 gcaactccgt tcctatggcc tggaatgggg tttgtccaga ctggactatt cgcctaaccc
     3001 ccgaaaaggc tggatgttct ccgttaccgg tatggcagga acacgaacca ttcgcagaaa
     3061 tgcatctatc ctgcaactcg aagaccccgc agatccttct tttgattttg ctaccctgta
     3121 tgacagcctg gatgagcgct cactgacggt tcagtatgcc tatgatctgc agtatgtctg
     3181 gcccttgtcc ggctcatttg tatggatgat gcgggcaaga ggagcgtctc agatcaaccc
     3241 cgcagtatat gccaatgaat tgtatcgtat tggcggcaat cagttgctcc gtgggttcga
     3301 tgagcagagt gtgctggcat cagattacca tatactgacc ctggaaccca ggtatctcct
     3361 gagcagaaat gcgtatgcag gtgtattcac agatgtaggt tggatcgcga accggtcgtt
     3421 gagccccgtg gtgaatgatt tgcctgttgg ctttggggca atttttacct ttgagaccaa
     3481 agcaggggtg tttggtctta cttatgcatt gggaacccgg caggatgcac ccgtactggt
     3541 aagaaatgca aagattcatt tcggttatat aaattatttc tgattgtaca gactggcttt
     3601 ccttatacta ctcttggtcc gggttgccgg attgcaggcc aatgatcagg tcattatgcc
     3661 tgcagacagt gcctggggta tgcagccttc agacgggacg gtttatgcac agcctgtcag
     3721 aacatcctca gaagaacagg gcgaagtggt gatattgctg ttgttgctgc ctgttttctc
     3781 actgtttgta tttgcattca gaccctatct gagcaccaat cttctggttt cgggcaggat
     3841 gcaactggca gatcagatca tgcgtgaaag ggaattttcc agcggattgc caggcctggt
     3901 cctgcatgtc ttgttttcgg tgtctgtcgg agtcttactt tggcatcttt ttcctgaagc
     3961 cgtggcaagc ctcctgccat ccggccatac ctggcagtta ctgatcctgt tcactgtctg
     4021 gcccttgttg tattggttca gatttctggt aagccaggtc ttcgggctga tcttccggat
     4081 gcggtcagcc atcggccgtt tccgctttcg tgctgatata ctcatagaaa ccaccggttt
     4141 cttcctgttt ccggtagttt gtcttctcat gctgtctcct tcagccatgc aggaaatctg
     4201 gctggcggct tctgccataa tcctgtcaat tcttgtgata tggcgctggt atgccctgct
     4261 gcgggtagcc ggaagtcttg cagggtttcg ggtttcctat tttttgctgt atttttgcgc
     4321 cttcgaaatc gcccctgttt tggttgtttt tgcccttgtc agcggccgct caggactgga
     4381 tttctaaacc tgtgaaaatg gcacaacagt ctgcagacct gaaaccggta aagactatcc
     4441 ttgtctctca gccacaacct gagaacccta aaaatcccta tgcggatatt gaggccaagt
     4501 tcaaggtcaa gatcgatttt cgaaaattca tcagtgtaga aggtgtgcct gccaaagaat
     4561 tcagaaagca gcgcattgat atcaatgaac atactgccat tattctcaca agcagaaatg
     4621 ccattgatca tttctttcgc atctgcgaag aactgcgcat cagaatgcct gaatcaacca
     4681 agtatttttg tcagagtgag gccattgccg tctatctgca gaagtatatt cagttcagaa
     4741 agcgcaaagt attctatggg ccaaatggca aggacaagga actgaccgac ctgctggcca
     4801 agcacatttc caaagagaag tttctccttc cttgtgcaac gcttcaccag gatgccatct
     4861 cggctttcct ggaagaaaag aaggccaatt tttcagaagc catcatgtat gaagcgagat
     4921 ttgctgacct gagtgatatg aagggctcta tcatgcatga catgatcatc ctcttcaccc
     4981 ccaaaggtgt agaaagcctg tttcagaatt ttccagagtt caaacaaggt gatacacgca
     5041 tttgtggcat gggagccgga acccagaaaa gcatggaaga tcatggcctg cgggtggaca
     5101 tcaaggcacc aactccggaa tcaccttcca ttttcatggc cattgagaat tacctcgaac
     5161 aatccaatcg caggaagaag aaatagcaac catttggtgc actgcactgt tgagttgtga
     5221 aaacctgatc atgcatcctt tacgttctga ccacaccaat acactggtgc atgccaccag
     5281 cccatacctg ttgcaacatg ccggtaatcc tgtgcactgg caacagtatg ggccagatgc
     5341 atttgaactt gcacgccagg aagataagcc tctgctgatc agcataggct atgccgcctg
     5401 ccactggtgt catgtcatgg aacatgaaag ttttgagaat cagaaggtag ctgattttat
     5461 gaatgaaaaa ttcatatgtg tgaaggttga cagagaagaa ctgccacacg tagatcacta
     5521 ctatatgagt gcgtgtcagg tcatcagcgg tcagggtgga tggccattga atatgttcgt
     5581 gacagcagat ggtaaaccat ttaccggagg cacttattac cctccgaaac ccggatatgg
     5641 aagaccctcc tggatggacg tgctggcatt tgtttcagat atctggaaga atcgaaggga
     5701 ggaagtgctg gaacaggcag ataaactttc ggcacatgtg caccagagtc aggcatctct
     5761 gttaaccctg aagcctgaaa gtgttgctga cggatctgat acggaaaaaa tattccgaca
     5821 tctgatgtcg caggcagatg aggtaaacgg aggctgggga ggtgccccaa aatttccggc
     5881 tgcaatggtg ttgcagttcc tgatcaggga agagtggttt cgtccggatg atcaggtcag
     5941 gaagcatgtg cagaagacct tgcgtcatat gcattatggt ggtatctggg atcaactggg
     6001 gggaggattc tgcagatata cggtagatgc atcctggctg atacctcatt ttgaaaagat
     6061 gctgtatgac aatgccttgt tgctggagac ttatgcactg gcttacgggc gatggcagga
     6121 tcctgaagac ctggaagtgc tgaacggtat cctggccttc gccaacagag agctggttgc
     6181 gccaggcgga ggctactttg catcgctgga tgcagatgca gaaggagaag aagggcgata
     6241 ttatgtctgg acgaacgaag aaatagaatc acttctggcc gatgatgcgg tctggttcaa
     6301 aaagcgtttt caggttagtg aagacggtaa ctgggaagga aggaatatcc tgcaccggaa
     6361 tcatcccgga ggaaagtctc cttcctcgcc cggccaggag gcggccatgg ctgcattgct
     6421 gcaggcacgg tctgaaaggg taaagcctgc tctggatgat aagatcctcg tatcctggaa
     6481 tgggcttttc ctccacgcca tgctcgtagc atatcgtgcc accagacgtg cagacctgtt
     6541 gcaggcaacc ctagatcata ccaaagcact tctggaacac tgtcatgtag atggtatcct
     6601 gtatcatgta tggtgtaaag gccaggtaac tgttcctgca aatctggacg atcatgctgc
     6661 cctgatcaga ttgctgctgg cattgttcgg agaaaccggt aataaatact ggtatgatca
     6721 ggcactatcg ttggccgagt ttgctgtcgg gcatttcagt gatccggaga gcccgctttt
     6781 ttatgatcac ctgccaatgg agtcgctgcc ggttcgcacc agagaagtat tcgactatgc
     6841 ccagccatcc ggtaacgcac ttttcgcaca aagtgcgttc acactgtatc tctatagcgg
     6901 agagcagcgc tggctggacc tgcaccatca actgacctct gccatgcaaa caggtatgga
     6961 acgtttcccg caggcttgcg ggcagtggct tcaggctgct cagcccctgc aacatccggt
     7021 agcagaactg gtcacagctg gcccggaagc acggtctttg ctggccgaat gtcatgcaga
     7081 atggtatcca ttgcttattt cactgccggt ttgtgacaga tcgcttgaaa acatgccatt
     7141 ttctaccgac agatttgatg ataatgtgac gaggatattc ctatgtaaag atcatcaatg
     7201 cgcagctccg gtcagcacgg tgtcagaata tgcgaaactc ctgaaagcgt tttgagcagc
     7261 ggcagattat ctataccttt ccaccaaaat atacgcccac ttccgggcgt ttttacttgc
     7321 atccggatca acatgaagag caggatactg attagtgcca tattgctgct gtgcagtatg
     7381 ttccgggcct ccgcccagga cgagccgcag ttttcgcagt atatggcagc gcctgtattg
     7441 ttcaacccgg ctgccgccgg tttggatcag gcctggaaca gtgccttgct ggtgcgtaac
     7501 caatgggtag gcttgcccgg ggcacccaca acccaggcaa tagccacaga gttgccggtt
     7561 tacagaattc ggagtggtat atcgcttcgg gttatgaatg accgtgcggg tcagcagaac
     7621 actacggttg ccaatatcgg gtacagctat cagctgcgtg caggatcagg cctgctgggt
     7681 ttgggggtac atgccggtat agccagcaga agtttggatg gaagccggct gatagcccct
     7741 gacggaagct acgaagcagg tgttgaccac aacgacgatc aacttccggt tacccttgtt
     7801 tcctcactga tccccgatgc cggagcaggg gtttttttca ggaatgaacg attgcaggca
     7861 ggcctctcag tgactcatct ggcaggttct tctttctcat ttgatgcacc cggcggcagt
     7921 gccggcatac aatatcaccc ggtaggctac gttttcgtgt cctggaaagt gctgcagcaa
     7981 gaaaactgga gcctggaacc gggcgttctt tacaagacag accttaccga acagatgctg
     8041 gatgccaatc tgcttttcag ctggcgtgag acacttcttg ccgggctgtc attacgcagt
     8101 ctggatgcag gaagaaggga tgcactggta gctatggggg gctggaattt cagcaaatac
     8161 tggggcatcc gctacagtta tgacctgacc ttgtcagcac tcaataatgc ccagtcagga
     8221 tctcacgaat tggtgttgac ctaccggtat ccgctggcac caccaagagc cggaaaagag
     8281 atcaataatt tgcggtatct gtattattaa agtctgttgg tgaattggtt atttgttact
     8341 aatttcatgc ccactttgcg gttgaggtaa gagtcggtcg caagggaaag tgttgcttta
     8401 tatagtataa aacgcatatt tttacgccat taagttgact aacttagcaa gtatgaaaag
     8461 aaagttgtca tttattccgg cattattcgc agggtcagtg atcttcctgt cgggttgtgt
     8521 ttcgtccgac tgggatgatt atcacgggca gctgctgggt atgcaggatc gccccgtgtg
     8581 ggagccgatc aacccttacg ggatggtcta tgtaccttcc ggtaccctgc acattggtcc
     8641 gtccgatcag gatgtgaaca actctctgtg gcagcgccag aaatccatct ctattcaggg
     8701 tttctacatg gacgacacag agatcaccaa caacgagtac cgccagttcg tgttctgggt
     8761 gagagactcc attgcccacg tgctgctggg tcactacgcc gaacaggaag aaggctatca
     8821 gactgaagtg ccagtgcttg actgggacga ggagatcgac tgggaagacg atgagcagcg
     8881 tccaaaactg caggaaatgc ttctgcctcc gggcgaatac acctactatc gctctgttga
     8941 ccccaaccgt ttggtatata cttatcagtg gatcaaatgg aaggatgcgg ccagcaagga
     9001 gctgaacaag gatcgtgaga agtacatctc tgagttcaag gatgagaaga ccgtaaaggt
     9061 gtatcctgat acccttgtct gggtgcatga tttcacctat tcctataatg aaccgatgac
     9121 ccgccagtat ttttcacacc cggcttttga tgactatccg gtagtgggtg tcagctggca
     9181 tcaggccaat gctttttcac actggagaac acaattctgg aactggtgga ggatcaagca
     9241 tgatgagatc atagtagatg atttccgttt gccaaccgaa cacgaatggg aatatgctgc
     9301 ccgtggtggc cgcgacctgg ctccttatcc atggggtggc ccttacgtga gaaataccaa
     9361 gggttgcttc ctggccaact tcaagcccgg ccgtggtaac tatccggaag acgggggtta
     9421 ttatactgtg aacgtatatt cctatgcacc gaatgacttc ggtctgtaca atatgtcagg
     9481 aaacgtagcc gagtggacca gcacggcttt ccgtgaaaat gcctacgcct tcattcatga
     9541 tgagaaccct gatatccgtt atgatgcgga agacagtgat ccgccggcca tgaagcgcaa
     9601 ggtgatccgc ggaggctcct ggaaagatat cagctattat atccagacag gatcccgtca
     9661 ctgggagttt caggatactg ccaagtctta tgtaggcttc cgctgtgtga tgccattcct
     9721 tggccgttca atgagtgact actaatgaaa gaaataaacc acctgtattt gacaacccgg
     9781 ccgtgatcgg ggagatttcc ttattgttta acaatcatta aaaacaaaac aaacaacaat
     9841 gtcagctttc tttgaatcag atcgttttaa gaggatcaag aacctcatca ttggtgtcgg
     9901 tgcttccgtg gtactggtgg gcgccctgtt taagatcctg cactgggaag gtgccgatat
     9961 catgctcatg gtcggtatgt ttaccgaggc ctttatcttc ctcctgctgg gtgtacttcc
    10021 tccgcacaaa gactactact gggagaagat ctatccggac ctcgacatcg ctcctgaagt
    10081 ggaagaagcc aaaaccggaa agaaactgaa caaactggaa cccaagaaga gtgtaaccca
    10141 acagctggat aacatgctgg aagaagctca ggtggaacca ttaatgatag aacgtctggg
    10201 tgaaaacctg cgcaagctgg gtgagaacat cgaaaaactg tctgatgtac aggatgcagc
    10261 tgtaagcacc aatgaataca gcagcaaggt gaaggaagcc actgcagcag ttactgatat
    10321 gcgtgaagca tacaacagtg ctgcagcagc catgtctcag ataacagctt cttctgtaga
    10381 taccaccaag taccacgagc aggtacagat ggtaacaaag aacctgagcc agctcaatgc
    10441 tatctacgaa cttgaactcc aggataccaa taatcatctg aaggctatga accgtttcta
    10501 tggcagcctc aactctgcta tggataacct gaatgagtca ctgaccgata ccaacaagta
    10561 ccgcgaagag atctccaaac tgtcaggtaa cctcgcctct ctgaaccgca tctatggcaa
    10621 catgctgaat gcgatgaaca tgggcgccgg aaataccgcc aactgaaaac ctcaggaatc
    10681 gattattcac aagtaaaaag gagttaaaaa atgtctattc ctaaggaacc acgccagctc
    10741 atgatcaacc tcatgtacct ggtgcttaca gcgctgcttg cgctgaacgt ttccgcggag
    10801 atcctgaatg ctttcaatct ggtgaacaag ggtatcggta ataccaatac catcctgcag
    10861 gataagaaca accagatcgt agccggtatt gccggcaagg cagatgaagg tgacgatccg
    10921 cgggctaagg atatcgccaa ggatgctgca ggcattcaga aggtaaccgc agatttctac
    10981 gcttatgtgg aaatggtcaa ggattcattg atcagttaca ccggaggtat gatcgaagac
    11041 aagcatcatc ccggccagga aaaactgaaa ggtgaaagcg atactgaaag acctaccacg
    11101 ctgcttatta ataaaggact cgctactgag ctgaagacca agatcgaaga gacccgtcag
    11161 gagtacgtga aactgctcca gaaatggaac ggagaaggta aggtcaacca gcttacactg
    11221 aatgtggaag atggtggtgg tgagcaggga cttagctggg aagaatccaa cttctataaa
    11281 gtacctgccg ttgctgctgt gaccatcctc accaagatcc agaatgatgc caagagcgca
    11341 gaatcaactg tgctggaaca catggccaat cagatcgatg cagccaagat caagttcaat
    11401 aagatgactg ccatggtaac cgctcctact tcctatgtga agcgtggaaa cgagtacacc
    11461 gctgatatct ttattgcagc ttcttctgac caggctcaga tcgaagtgta caccggctct
    11521 tttactgcag cagtaaagaa agatgaattc gaccagttca ttgaattgga aggatctgca
    11581 ccaccactca acaaccctca gaaaattgat gtggttggtg gtatgggtaa gatcaaggaa
    11641 actgccggcg gccagcggaa tttccagggt gtgatcagca tcccggatcc tgtgaagccc
    11701 ggtaacttca agttctatcc gtttgaattt ggttatgaga cattcgaggt tggtgaagcc
    11761 gttgtgtctc caacagccat gaacgttctt tatattgggg tggacaatcc cattaagatc
    11821 tctgtgcccg gttatacctc tgataaagtg accgcctcag gttgcggtat cagcaaggtg
    11881 aaaggtgaag agtatgtggc tcgtccttcc aatgtgggta aggagaacat caacgtgacc
    11941 gtgaatacag gcgaaggcac caagagccac agcgctgaat tccgtatccg tcgtattcct
    12001 gacccgtatc cctactgtgg aacttccaaa ggtggtacca tgcgtatcgg cgaattcaag
    12061 caggtcagta ccatcgtagc caagaaccct gacttcgtat tcgatatcaa ctatcaggta
    12121 acaggatttg aaatggtgta cgctccccgg agtggtggta acgtcgtttc cgatgtgtcc
    12181 aacaatgcca aattctctaa gctgatggac gatatcaaga cgaaagccaa gcccggcgat
    12241 accatagtat tcccgcgtat ctctgtgaag atgccggatg gtacgacccg ccaggtgagc
    12301 ctttcattca aactgatcgg ttaaaaaaac agcaagtatg aaaacagtaa tcaggtcggt
    12361 atttgcattg gtggtgatct tcaccgctac tgctgccatg gcgcaaccaa cttccgaagc
    12421 caagcgtgac agagcctatg acagggatct gctgccacag cgggaagtga taccctatga
    12481 ccatatccgt gaagcagatg tgttctggga gaaaagggtt tggcgccagc tggactttcg
    12541 cgaaaagatg aacctgccat ttacctggcc ggtagatcct ttcatggatt tcatctacaa
    12601 ggctatcatg aacggcgagc tgactgccta cagccctttg tatgatgatt tcggcgaagg
    12661 aaccgccctg accatcgagg atatcaagaa caaatacgaa ggtgtggata ctacctacac
    12721 ctatgatgat gagacctacg aggaaaagat cctcctgatt ccgcgtcagt tcgactatca
    12781 gaacattaag aagatgcaga tcaaggaaga ctggatcttt gacgaagaaa cgtccaccct
    12841 ggtggtgcga atcatgggta tggctcctat cattgaacgt atcgatccgt ttaccggcga
    12901 acccatcggt gatgagatca tgttctggct gtattatccc gatctgagga atatcctggt
    12961 gcgccatgag gtgttcaatg agaagaacag tgcacgttac atcacctttg aagatatctt
    13021 tgaaatgcgt tatttcagca gctatatcat caaggaagac aatgtgtatg accgcttcat
    13081 caactcttat gctgccggta tcgacctcgt gctggaatct gatcgtatca agaatgagat
    13141 cttcgaattt gaacaccagc tctgggagtt ctgaccgaag cacattaata gaaaaagggc
    13201 cgttgaaaga cggccttttt ttattgttct gattcccaat aggcaaacag tgctctggct
    13261 ttggcggggc caatttcttt ggcaactgtt tcctcgccag cttcccggat cttttttacc
    13321 gacttgaatg tcttgagcag tagtttctga cttgcaggtc ctatgcccgg aataccatcc
    13381 agtccgctcc tgatactttt cttgcttcgc tgctggcggt ggaaggtaat ggcaaagcgg
    13441 tgcgcttcat cacggagctg ctgaatgaca cgaagacttt cagatttctt gttgatgtac
    13501 agaggcagag gatcttcagg aaaatagatc tcttccagtc tttttgcaat ggagcatatg
    13561 gccacctgtc catagatgcc cagctctttt aaagattcta cggcggctcc gagttgccct
    13621 ttgccgccgt ctatcacgat gagctgtggc agcgattcac cttcttcggt aagccgttta
    13681 taacgacggt aaacgatctc cttcatgctg gagaaatcat caggacctac taccgttttt
    13741 atattgaaat gccggtagtc cttcttgctg ggccttgcat tcctgaacac cacacaactt
    13801 gcaaccggac tgcttccctg gatgttgctg ttgtcaaagc actcgatatg atgaggtctt
    13861 tcctgcaaac ggaagtcttc tttcaatgta tccaggatgc gcaggttggc atttttatct
    13921 tcccgttttt gtttgcctgc ctgtcgctct tccctgagat agagtgcatt cttaaatgcc
    13981 aggtccagca atttcttttt atcgccggca agcggaattg ttttgatcag gccatcgtca
    14041 tcaggtgaac aatcgaacgg tatgataact tccttgcagg agctctgcat ttcacgacga
    14101 agatggttga ccgcataggc cagcagttct tcccgcgatt catccagctt tctttgcaac
    14161 accacggtct tgcttctgat gatcataccc tcgttgacct gcagataggc aatgaccgct
    14221 tccttctcat tttcggtgta tccgaacaca tctacctcat ccatgcgaac attgaccact
    14281 gtgcttcggc cctgaaaggc atcgatcgct tccagtttgc ggcgtatgtc ttctgcctgt
    14341 tcaaatgcca gtgcttctgt gtattcgtgc atcctgctgc gcagttcagt cttcagttca
    14401 cccatatgtc ctttaaggat gcggataact tccttcacat tggccatata gtcttcttca
    14461 gattgcagac cctcacaggg acccttgcag tttccgagat ggtattcgag gcacactttg
    14521 aatttgcctg ctttaatatt tgcctcactc agtgcaaggc tgcaggtgcg catgggatac
    14581 aatgattgaa tgaattcaaa aacggtacgc acccttccaa gggaagggta tggcccaaga
    14641 taagtggagc catcgtcttt gcgattccgg gtaaaaaaga tccgtgggaa acgttcattc
    14701 ttgacaacta tatacggata ggtctttccg tccttaaggt tgacattgta tttgggctgg
    14761 tattgtttga tgagggtgtt ttccagcagc aatgcatcct gttcagaatt ggtaatggta
    14821 aattctatcc ggtctatctt tctgaccagt agtttgattc gtgcagcgtg atcacttttc
    14881 tgaaaatagg agcttaccct tttgcgcagg tgtttcgctt tgcccacata cagcaggtta
    14941 ccatccgcat catagtagcg gtacacgcct ggttttgcag gcagtgtcgt ttccagttct
    15001 ttaaaagctt cgggtgtcaa atctttgcaa tggcaaggta aaccatctgc aggaaaaggg
    15061 caacagtatc agttgtaacc ttccagcaga tcttcataca catcctgctg agaggttttg
    15121 tatttgctgc agtctaattc aatggtttgc gggccctcat atctctggaa cttggcattc
    15181 ggatctatcc caagggttgg gtcgctgtac acccgtttca tgaataatcc ataaatgggc
    15241 agtgccatgt tggctccttg tcctaaggca gtggaacgga aatgcaccac cggatcgtcg
    15301 gcgcctaccc aggtaccaca aaccagctga ggtacaacac ccatgaacca gccatcagtg
    15361 ttttcctggg tagttccggt ctttcctgca acttctcccc gaattcctcc gtaggcatcg
    15421 cgacgcagac ggttacctgt accgtagttg accacatatt ccagcatgtt cagcatgacc
    15481 gaagcgatct cctcccgcat gacctctacc ctttgggtgg ggaattcagc aatgatgttt
    15541 ccgttcttgt cttcgatgcg ggtaatgaat accggcctga ccgccacacc tttattgacg
    15601 aacgcggtat aagcagaggt catttcgaat acagatatat ctgatacacc caggcagata
    15661 gagggctgaa aaggcatttc actttcaata cccatattgt gagccaggtc aatgacgggt
    15721 tgaatgccca cttcacgaag gaggtaggct gtaatggtat tgacagattt accgagtccc
    15781 attgacaggg tcagcatatc gccgtactta ccatcgctgt tcttgggggt ccagtctttc
    15841 tgcaaccccc atcggctgcc tttttcaaag gtgaccggca ggttaggtac ttcctgacag
    15901 gggctgaaac ctttatcagc aatggctgcc gtataaacaa aaggtttgaa ggtagaaccg
    15961 acctgtcttt tggtattgga attcacatga tccagctgga agaagcgcat gtccacaccg
    16021 cccacccagg ccttgatctg acccgatgcc ggatcaactg ccatgaaccc cggatggagt
    16081 gttttcttaa cgtatctgat ggagtcatac ggactgatca gggtgtccag ttctctgttt
    16141 ggggcagtcc agttgaagag ggttgtttca acgggcgtat ggaattcttc ttccagttct
    16201 ttttctgact tacctgcttt tttgcccacc cgccagcgct ctgattgttt catcgcctga
    16261 tcgatgagac ccggttgtgt tcttccccga tagggataac tccatgggtc gtatttgttt
    16321 ccttcccagt gtttgttgaa ctcatcctgc agtttggtca gccattcggc agaggcttct
    16381 tctgcataca gttgcatacg gctgtcaatg gtggtataga cctttagccc gtcacggtat
    16441 atattccagt tggttccgtc gggtttcgga ttgttttcag cccaatctgc cagccacagt
    16501 ctgagatact cccggaaata ggttgcctca cctgacgtat gatcctggcg tttgaagcgg
    16561 gttatgtcaa gcggaagttc tttgatagaa tcataggcgg ccacgtccag gtaaccggct
    16621 tctttcatct gatagagcac cacattccgt cggtcgcggg tacggtcagg gtaacgaacc
    16681 ggattgtaat aagatggatt gttgaccata cctacaagcg tagcggcttc ttcgatcagc
    16741 aacgaatcaa cgtctttgtt gaagaaggtc ttggaagcgg tctggattcc ataagcatca
    16801 tacacgtatt caactgtgtt gaagtacatg gtgatgatct cttctttggt atatctgcgt
    16861 tccagtttgg cgctggtgat ccattccttg aatttgatca gacccagaga aaagatgctg
    16921 ctgctggtgg ggcggccata gaggtttttg gccagctgct gggtgatggt agagccacca
    16981 cctgcatttt catttcgcag gatgatagtc ttgaaaagca cacgtgccag gcttcggccg
    17041 tcaatgccgg aatgatcata gaaccgaaca tcttctgtgg caacaagacc ctgtacgaga
    17101 tagggaggaa ggttctcaaa ggtgatattg gtgcgattct ctttatagta tctgccaagc
    17161 agttctccgt cggagctcat gacctgagta gcgaggctgt tttgcggatt ttccagctgt
    17221 tcaaagccgg gtaaggcacc aaaaactccg aatgaaagca gcacaatgaa gagtgtgaaa
    17281 gccagtattc cacctgcaac agtaaaccag aaccatcgca ggtaggtgcg gtattgtttg
    17341 aattcttcgc gaatgctcat tcttctgtca ggtagttttc ttcaaagaat ccgatatagg
    17401 tttcagcctc cttactctta tacagttgta cataatttga ttttgatacc acaaagaaaa
    17461 aggtatcatt catatctatg tcttcaaaga tctcttcata ttcattttcc acaaaactga
    17521 aatagtccat agcatctgaa gcatcagtga atgtcttgat caggatgagc gtttgctccg
    17581 gtccgaacag aagtgagctg acccgcagtt tccgcacatt gtagttggtg gtattaaaat
    17641 ctgcgagggc atttttgatg ctggtagctt cttttccctt gctgggaata accaggatca
    17701 catagtgttc ttcttcgctg ttatagctgt agagttcaga aggatcaatt gcctcttctt
    17761 tggcctcggt cttgttgatg taatcaagaa tagctttggc acgaatgcca acttcatctg
    17821 tcgggtattt atctacgatg ctttgcaggg cagctgcgaa ggtgccaata ctggcagtat
    17881 ctgagagaga gagagcttcc agcatatcaa atttgggctg gagcggatta ttggggaaga
    17941 ggctgtctgc tgcagtcaga cgcattctta cagttgacag atctcctgcc gtataaaagt
    18001 cataggtggt ggtatagtag tcttccagct gttcattttg cttcagttgt ttttccagat
    18061 agtcaggatc gcggatgata ttggcgaaca ggctttccgg atatttattc agaatagaag
    18121 acttatactg atcttgtgca ggttgaccat caaagatcac aaagagctga tagagcgatt
    18181 gcagttcaaa aggattgtca gggaagtcat tgatgttttc gcggaagtac tcaactgctt
    18241 tgttgtcatt ttcgaatttc tgcctgtata tattccctgc cgcatacagc gcaccggcaa
    18301 ttctgcggtt ggaagcctcc agcagttcag gggtgcgggg aaggctttta atgatatctt
    18361 ctttggttag ttgttcgctg ttcagatcta tttcagattc ttcggtagca tcctggtttt
    18421 cattttcacc gatggtttct tcatcaaagg agcgcttatc agatctacgc cagttatctt
    18481 cgagagagcg gttaccccat acctttttga attcgccgaa acctctggaa agcagggtgg
    18541 cattatagaa atagaattca cctgtattgc cttcattgcg gtcatcgcct gctgcggcac
    18601 catccagaaa ttcctgttgg ttggcagctt cctgttcggc tatcattttg tcgagttcct
    18661 tatccaggtc aaactcattc aatgatgcga ggtattgtaa tttcttttct gtttcaatgg
    18721 tagtaagtgc cttgacgaga tctatgagac catcgcggcg ggtctcgatc tccttatagc
    18781 gggcaaatgc acggtctaat gtttgcagtg cgctgtcata ctgattgtag gctggtttgt
    18841 attcagtctg ttcgtaatag atgtcgccca gtttgacata actaagtgct ttttcagctg
    18901 ggttcacatc gccggttctg ataccttctt tcaggtagtt cacggcgtct tccacattgg
    18961 aagagcgaag ttctatttcg gccagtgtat tgtagataag cggatagaat tctttgtatt
    19021 tatcttcctt cagcatagct gtaagggtag aaacggcagc agaagatgct gtgcctgatt
    19081 gctgcatgcc ttgtgttgct gtgaacaggc gggcatagaa atcggtaaca tagtcaggtt
    19141 tgtatttaat gacccgctgg aatccttcct gcgacaagga gaattcgccg gcatctgcat
    19201 acagttgtga taagatgaat gcatagcggg ctttcagagc tttgtctttt gtcagctctt
    19261 cagctgtttg cagtgcttta atggcggcac cgtactgtct ttgtttcagg tagagactgg
    19321 cttctactgc agccagttca tcatccagct gtgacgggaa tttcgggttg cctttgatga
    19381 cactcaaagc agttttggct tcggtatagg cacctttctc tgtcagggtc aacaccaccc
    19441 acagagcagc ctcgttggct accggatgat gtgacaggtg ttctttcagc caggattcct
    19501 catcggcact ttttttcttt ttctttttct tccgctttcc ctggtcgctg tacttactta
    19561 tgatatactg aaagctggtg atcgcttcgt cgtattcttt tttgtagaag ttggcctggc
    19621 cgatcagcat atagcaatca tccacccatt tgctcttctt gtgaagctga ataacaatgg
    19681 aagatttctt gatgacctca tccaggttag actgttcacc ctgggcagat tccttatctc
    19741 cgaacttata aaccggaagt aaggccgaga agtcttctgt ttgtccgtcc cacaaggttt
    19801 gccggctttg agacagcagc aatttggcat tgaaatagcc gttatctctg gcggtcagat
    19861 catggtacat ccgcgatata agcgggtcct tcttagattt gctgcccgat gcgcaggctg
    19921 agagcaaaaa aaagggtaaa attgtaagga ggatatagat ggatcttctg atcaaaagag
    19981 cacggtttaa tggccggatt agtataaccc gatgcacaca aaaatattat taaacaggca
    20041 gaaaaaataa cccatccgag ccaatatgag cacaccatcc ggtgagaagc agaactggcg
    20101 ccagcggttg cagaacaaat accggctggt cattatgcac gataagacct ttgaggtgga
    20161 aacctcggtc aaattgagca tgctgaacct cattgtgctg ggcagcacat tgctggtggt
    20221 catgggggtg ttggtgtaca cggtcatagc tgtgacgcca cttaagaact atgtttcggg
    20281 ttattctgag gtgtccacta cccgccagtt gctggagaac agtgaaatag ccgatagcct
    20341 gatgcgggaa atggaggcca gagatgccta tctggatatc attaccagaa cgctcaaggg
    20401 tgagctggac acggtggtga acaagccaga cagtgctgcc ataccgcagg aacaactgaa
    20461 tattgatacg atctcagaca tagaccgggc cctgaggcag caggtagaac aggaagacct
    20521 gtttaacctg acaggtgcag acccccggca aacaggaaaa gagaacttca gagccctgta
    20581 tttctttcca ccggtaaagg gtacggtgac cagtagtttt gatccgtcac aaggacatta
    20641 cggaactgat attgtagcca aagagggtac accgatcaag acctgtctgg acggcacggt
    20701 ggttgacagt tactggagtg tagaatcggg caatgtgatc gtaatacagc acagttacgg
    20761 gctgatctcc atgtataagc acaattctca ggtattgcga aaagcgggaa gtccggttcg
    20821 ggcaggcgat gtgatcgcca ttattggtaa caccggagaa ctgagttcag gcccccatct
    20881 gcactttgaa ctatggcaca acaaagtggc tattgacgca gaggagctga tcatttttaa
    20941 ctaattttga acatcatgtt cggcaacaag caatcagcag attctaccgg caatcctgga
    21001 aagggtatgc tggtttccaa tcgtatcaat cacggtaccg agctgaccgg agagttggtc
    21061 agtgatggtg atatacgtgt ggagggggtc attcggggca ccatcaaatg caaggcaaag
    21121 gtggctatcg ggccaagtgg tcttgtagaa ggagatatct tttgcaagtc tgctgatgtg
    21181 gaaggcaggg taaaaggaga tgtggaagta gccgataccc tgatcctgaa agctacctcc
    21241 agcgtggatg gaaacattca aacaggcaag atcgtggtag agtcaggtgc ccgtttcaat
    21301 ggtatctgta acatgggtca aagggaaaag aaagtacatg tcgaaaagcc cgtcgccaga
    21361 accctccaga ccgaagcggt cagttgatca gtatcttcgg tacagcaata ttgcctttca
    21421 actttttgca gccattttca tcggtgtttg gggtggtatg aagctggatg cctggctgca
    21481 tacagatcca tggttcaagg ttatcttatc tttgctgggt gtgagtgccg gtttatacag
    21541 cgtgctcaaa gaattcatcc aacccggtaa aaaatgaaaa aattcctgat cacctatctg
    21601 gtgttcacct ttctggtggc ttgtgcccta tggggtattt tgcaaattcc tgtgttacac
    21661 ccggcttctt cctttgcctg gatctgtttc gctgttttct tcatattgac cctgctgacc
    21721 ttctggatgg cggccagaaa tatggaagga aggtttcagc aattcatgaa catctttttt
    21781 ggcgggatca tcattaagtt gttcgtaacc ggagccatgg tactcatcta taaaagtgtt
    21841 gccggggcac ctgaacaatc ttttgtgtta atagcacctt tcgccctgat ctatttttct
    21901 ttcctgggct ttgagacctc acagttgctg agattgaaca ggaaacagac cggaagctga
    21961 tggtacaatt taatatcagt ttgccctgta gaagttagag ggatcaacac tcatccggtt
    22021 tccgtggggc agaaattctg taggaaaata ggaggcggaa aaagtgatcc ggattggatt
    22081 cgaaccaatg acctactgct tagaaggcag ttgctctatc caactgagct accggaccgg
    22141 aggtgcaaag ttagacaatg cacagattac ctgttatggt ttcctgcttt ctctttgctg
    22201 gaattctctc aatcccagat tccacatggt gaaactcatc caatcagcgt cttcttctat
    22261 gatctttgcc agtggttttc cagctccgtg accggcatct gtatcaattc gtatgagcac
    22321 cggattatcg ccggcctgag ctgcctgcag ggtagctgcg aatttgaaac tgtgtccggg
    22381 caccacgcgg tcatcatgat ctgcggtggt gatcagggta gcggggtagc tggtaccggg
    22441 tttcacatta tgcagtggtg aatatttgat cagatactgg aactgggtag agtcatcgct
    22501 gcttccgtat tccactgccc aaccccatcc tacggtaaac ttatggtaac gcagcatatc
    22561 gagtacacct acgcccggaa gtgccacacc aaacagatcg ggtctctggg tcattgcggc
    22621 acctaccagc agaccaccgt tagacctgcc ctggatggcc agtttttcac tggaagtata
    22681 tttctcaccg atgaggtatt cggcggctgc tatgaagtcg tcaaacacat tctgtttttt
    22741 ctccagcata cctgccttat gccattcttc tccgtattca ctacctccac gcagattggc
    22801 taccgcatac acgaatccct gttccagcag cgctatgcga ctggtgctga agctgggttt
    22861 gatagcgata ttgaatccgc catacgcata cagcagacaa gggttggtgc catccagttt
    22921 cagacctttc ttataggtca ggaacatcgg gatcatggtg ccatccttgc tcggatagaa
    22981 gacctgtttg gtctcataag cggaggcatc gaactggact tcactcttct tatacagggt
    23041 tgacttacca gtattcacgt cataggaata gatatcagac ggggtagtga aagaggtgaa
    23101 attgtaaaat acgttgctgt cggtcttttc gccgctgaat ccaccggcgg ttcccagacc
    23161 cggcaattgc acttctcttt cctgtttacc ggtgcggtca aattgtacca cccgggtagc
    23221 cacatctttc aggtatccgg caaaaagttt cccggcagag gcattgaccc attccagcag
    23281 atcttctttt tcgggtatca cttcggtcca gttttccggt gcaggatttt tcggatctac
    23341 tttgaccact ttgtagttgg gggccatata attggtgagt accagcagtt gatcaccttc
    23401 attatcaagc acactgtact cccagtcgaa tccttcaaac agggtgcgca attttttgtc
    23461 attactggtc aggtcacgcc agaggatccc ggtaccgctg gtgccttcgc tcatatatac
    23521 gaacaggaaa tgttcatcat ctgtggtact gctgaagaaa tatcttttcg gatgcgacgg
    23581 gtcgctgtac accagttcat ctgccgattg ttgggtaccc agtttgtggt aatacacttt
    23641 ttgcttgtcg ctggcaccgg tataggtctg attttttccg ggctcatcaa agcggctgta
    23701 gaagaaacca tcgccgtacc aggcggctcc gccaaacttc gaccattcga tcttgtcatc
    23761 cagctgggta ttggttgcta tctcccgcac atagagttcg gtccagtcac tgcctgcctt
    23821 gctctggcta agtgtcacat attttctgtc agcactgaaa ccggccatgc tgagtgtaac
    23881 tgtgccatca tcagaaaggg tgttggggtc aaagaatacc tgttcttctc catccatacc
    23941 tttcttgtaa aaggttacat actggttttg caacccgtca tttttggcat agaaataata
    24001 ttcgcccaca tggaacgggg tggacatttt cggatagttg gccagttctg tgagccgttg
    24061 ttccagctgg ttacggtagg gaatctgatc caggtagccg aaggtcacgt cgttttctgc
    24121 tgtgatccag ttgcgaacag cagaatcacc ttcgttttcc agccagcgat atgggtcgct
    24181 gatgttggta ccgaagtagt catcgttttg atcaatggtg gctgtttgag gataagagac
    24241 cttgtctggc ccttgtatat ctgtagtttg aacggattcc ttgcaagcgg caaccagcaa
    24301 caatacaggt actattatcc aaaatcgttt catagcaggt aatttgatca aagatatctt
    24361 ttgtatttgg tgaagaattg aattttgaca tgttttaacg ttcgctgaac gacagccacc
    24421 gcgtcatatc attgcgggtg atgatgccca ccaacctgtt gtcacgcacc accggaagac
    24481 tggtgatctt ttgtgttctc atgatggtca gtgcgtcttc gatcagtgca tcctgtctga
    24541 tggtctgtat ggtgcgcttc atataggtac gcacgctgtt ggtttcgggg ttgatgccgg
    24601 cggcttccag ctcagcaatt ttacctgagg tgatgagccc taccagttcg cctcgcctgt
    24661 tctcaatcag catgtggttg atagaatgcc attccatcac cttcttcaca aaaagcaatg
    24721 tgtcgtccgg aaatacggtg atcaggtctg aactcatcag ctggttaaca gtggtgaaat
    24781 gtctgatgag atatttattg gctttatcca ggtgtacatc tttccattcg gcaaccggca
    24841 attgctgtac ctggttttca tagatggccg cggtcagggc aatagcagcg tcagacgggc
    24901 tgagttgctt gcgtaaagtg cgaaaatttc taacctgcca ttgtgaaccg gtttgttttt
    24961 tcagtcgccg ttcaatggta ttcaggtgat agctgatctc ttcctgcggg atgcctgccg
    25021 acattaatcc tttggcagcc agcggaagca gttcctccag gatcagtgtt cttgtgttca
    25081 gttcttttcc ctgccagatc aatacggttt caattccggc acgggcagct ttgaagaaat
    25141 tggatttaat atcgcgaaag ctatgtgttt cagacaccgg agcagcaggc tttgccagca
    25201 tcagtccggt ccagaacgcc atgttggcaa cttcatcgcg gatgctgggg cctgcaggca
    25261 gatagcgatt ttcaatgcgc agatgaggtt tgccccggta cactccgtag cagggacgat
    25321 tccagcgata gacagttccg ttgtgtagtc tgagagcccg cagttcagga atgtggccgt
    25381 tgcggtattc ctgtaaactg tctggcggat catcaaaggt gatcagtacg tcaaaatgtg
    25441 ttatatcgtc tttatatata tcagtaatgc tgtttcttat ccagtctttt ccgaaagtga
    25501 ccctcggtct ctgatcgcga agttcttcta cactttttct ggtttctata ctttgctgaa
    25561 agagagcgat ccggatttcg ctccacaact cttttccaaa aagtaaaggt gaattggtag
    25621 ctgcagcgag taccggtgct gcgatcacct gtgaccagtt ataggcctga acgaaatctt
    25681 caggagctac ctgcaggtgc aactgaaaac tggtgttgca ggcttcgaac atgatgttgt
    25741 cgtggcgaat atggatctca tccacacctt tcatgaacag gtcaaactgc ttgccccttt
    25801 ttctgcgcag ctgtttgtta agcaggtgat acctggggag gggtgtgaga tagtccaatg
    25861 tgacctcatg cctggagatg gtgggcagga tgccggtcat gataacacga gaatgcagca
    25921 cagatgctgc tttctgagct ttgatatatt tcagttgcaa tccttccagc atacgatgaa
    25981 aacaacctgc ctgcaggtcc agcgggtcgc agttgagttc aatattatat ttggccagtt
    26041 ctgtagtgaa gcaggaatcc tggaggcccg ccagcacttc taagttgtta ttatcgggcc
    26101 tccactcatt gttgacgagg cacatttcct gttcagcacc tatgcgttga acatcatttt
    26161 ccagagcgtg gtctctgatc attagttcca gtgcttccag gtcatgtatc agggcattga
    26221 caaatgccat ctggttgtgt cctgaggtat ggatatggct actctggtcg cccataggat
    26281 ccacaagtta actatgtgtt aagggaagcc caaatgacga atatcaccgg cttgttagat
    26341 ttgccagtaa tatggcccgg gaagacattc aattggaagc aggctggaag gcagttttag
    26401 cagatgagtt cgataaagcc tattttcagc agatcaggta tttgctgctg gctgcaaaac
    26461 gagatggaaa ggtcatcttt ccgcctggca gccagatatt caatgcatat aacctgaccc
    26521 ccttcgaaca ggtaaaggtg gtcatactgg gtcaggatcc ctatcatgga ccaggccagg
    26581 cccatggtct ttgtttctct gtacctgaag gtgtgaaacc accaccctca ctcgtcaata
    26641 tctttaagga gcaggaaaag gatctgggca taccaaggcc ggcacacgga aatctggaaa
    26701 cctgggccag gcaaggtgtt tttttgctca atgccatgct aaccgttgaa tcaggaaagc
    26761 ctgggtcaca tcagcagatc gggtggcagt attttacaga cgccaccatc aaagccttat
    26821 cagaaaacaa ggagcatctg gtgttcctgt tgtggggtgc atttgcacag aaaaaggctg
    26881 aacttattga tgcaggcaaa cacctgatcc tgcaggcggc acacccatca cctttcagtg
    26941 ccgacagagg attttttggt tgcaggcatt tttcgaaggc gaatacctat ttgcaggaac
    27001 atggtatgca aa
//
LOCUS       DAOMAX010000007        26553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_191_length_26553_cov_4.729300, whole genome shotgun sequence.
ACCESSION   DAOMAX010000007 DAOMAX010000000
VERSION     DAOMAX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26553
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191_length_26553_cov_4.729300"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..3948
                     /locus_tag="PK742_01045"
     CDS             97..3948
                     /locus_tag="PK742_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQU75202.1"
                     /translation="MKQILLFLLLAGQFVQALTQVNAPNRTDKEGLRTGEWVIYFQQD
                     DQVTDNPSGATRYYLVNYKKGEPKGFTREYAIDGTLVWEGELRSVLPDTIHGVSRGFF
                     RDGTVSYEYTFADGLMQGPAKQFYENGELKWSCNYTDDFPDGTYKEYYDNGNLMREGP
                     VVKGKKNGYFRFYFIDGNMQKGVTYVNDVMDGPYDEYDNGKLKYHYAMANGLREGLVT
                     QYDASGQEENYYMFHADSVIGIKEVIGIVSSNLLPGMDQRLGLNMCLLMEEYFRKKYG
                     EDSPLYPFAASSLAQYYFTLGDEQNGHHWTIRSYELEKAQMGTDQEASSDKWHLLGLM
                     LESYAESDKAWECYRYAIRGSYEKGKPTETTVSYMSRAASMAVNLGHEQAGFEQFDAL
                     LLTCDTLGKDGAVVCTDAALNYISFLSAAFRDQDALHILESRRKIAKEAGSLNAWDLA
                     TADVIMQLNREDEAMDIYGRLYNNFTADRDTAQHIEIASSMAEYQMQSGNYAAAEKLW
                     LETVALSAAISDEDSLDYYSRLDDLATYYDRVGRKFQAIQYSQQVFGFRLRMAKDTTS
                     MEYIFFGDALTRELAVNSALSLGRLYTNVGMYDEAGSMFTYALDESLALHADTSFEYA
                     YALEAMAGYQMDKEDFDASEHSYLEAIRLSDTYYADINVNGTTWKDNLSELYMRMHQP
                     EKALVVAEQVLSDRQQQYPEGHPILTASYSRLSGILGALGQTDSALTLYEKVLDAEVA
                     RLNETFAVLSAAEQEDYLTTFRFDFDIYNDMVVRYAKPGSLHFQRMLNYQLLNRSLLL
                     YSGTAARRSMEQHPDAAIRSMYQEWMETGLYLSGLAGRETDQEVPSSLIDSLQQRTEQ
                     LEKDLTLRTGQDITFVPSVTAHALSSALQEGECYVLFLRYPVFEEAHVKHWELGALIL
                     NDTGNTMFVSIGKEATLEKLLENKEGESVQDRISRLYTFPELDEDSLLFQGDRVFATT
                     WKPLMPFVQHSKRVYYTPAGIMNLVNLSAVPDKLDHFLFQQMELIQLAGPEAVVQRAT
                     ESTLKSNATLTMIGGVQYDASDDALRSAVKGVETASTDIPLSAESSVITRGDNWKYLP
                     GTLAEVQGIDRQIKETKWKVEEVTGTLATEELVRGFSGDHAPDILHIATHGFFLDNSQ
                     GDPLHRSGILLAGGNRSWQGDRPSGGLQDGIFTAAEVSYLDLHKTKLVVLSACETGLG
                     AVDANEGVFGLQRGFALAGVDHTIMSLWKVSDKETALFMQTFYGHLAAGLSIQEAFAR
                     TRDSMSKRFAPYYWAAFVLQQ"
     gene            4076..4447
                     /locus_tag="PK742_01050"
     CDS             4076..4447
                     /locus_tag="PK742_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQU75203.1"
                     /translation="MKELTKAEEQVMQILWELEKGFIRDILERMPDPTPAYTTVSTIV
                     RILETKGFVGHHSYGKTHEYFPLVSREEYRKFITGGLLHDYFGGSVSNLVSHFAQKED
                     LSAKELDEILRVVQRSKKQKP"
     gene            4444..5640
                     /locus_tag="PK742_01055"
     CDS             4444..5640
                     /locus_tag="PK742_01055"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQU75204.1"
                     /translation="MSTEVLFIKVQLAMLVLYGFYKLLFSASVHHQVKRFYLLAIIPV
                     ALLHPYFFRGSAGGETLGRVQVLDTFIVQTVDQSMNTLDRNITWGIAEWVYLLITAAL
                     FLVLMIRVVRLLWMAWRLPKEVVGNKRIIRIPEDHSPASFFSLVFMPEGLSIDTGKVI
                     LDHEMVHVRQLHSVDVLLYEFVRCLCWFNPVAWLLEREIRLVHEYIADEQAGKDAPHF
                     YRDTLLAYRLGIEPALLTNNFYQSSTLKKRIMMLQESSSWKTTVIRLALLLPLLGMFS
                     LINTPAAAQTSTDKVYQQVEQMPEFPGGMDGLIKYLSEQVVYPESAKKDGVFGRVMVQ
                     FVLDAEGHVTQAQVVKGAAKPLDEEALRVVNAMPDWRPGMQDGKAVSVQMVLPIQFSL
                     DEPVKK"
     gene            complement(5644..6984)
                     /locus_tag="PK742_01060"
     CDS             complement(5644..6984)
                     /locus_tag="PK742_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75205.1"
                     /translation="MNHWPDGFEQHLQQQPGVQFEQLLQSLEQEAVVSVRINQRKPVI
                     RFHGLPLVPWCTHGIYLPERPVFALDPHWHAGAYYVQEASSMFLDHVVKQLFTHEPVR
                     ALDLCASPGGKSTLLCDALPSGSMLVSNEVIRSRVGTLRENITKWGHEQVVVSSADAS
                     AFAGLPSFFDLIVVDAPCSGSGLFRKDADAMDEWNPDNVALCAARQNRILDDVLPALQ
                     TGGVLVYSTCSFSEEENEAVISRLLKDHHLEIVHLEVPAEWNILHCGPEEGCFRFYPD
                     RLQGEGFFLAVMRLRENSPADRSFRPQKLPERSTAYDEQAWTDMTGRADGCFYSWDED
                     TWRWPLQREHDWLQICAAVPVVQAGVLAGRQKGKDLLPGHGLAMSGLYHDEIPVIYLD
                     EKQALKYLRREELDPALFESGWSLVGFNGAILGWVKKIPHRINNYYPMGYRLRK"
     gene            7029..7751
                     /locus_tag="PK742_01065"
     CDS             7029..7751
                     /locus_tag="PK742_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU75206.1"
                     /translation="MNFSKLSVLVPAYNEEATIVRILDTLQSVQLPEGILMELVVVND
                     CSTDRTGQIVEQYIAQHPDASILYEKHAKNAGKGAALHTAIQLATGDLIVVQDADLEY
                     DPHEFSILLKPFLAGQADVVYGSRFMGGNPHRILFFWHSIGNRFLTFFSNMFSNLNLT
                     DMETCYKMVRADILKSLVLKEKRFGFEPEVTQKLARIKGIRIYEVGISYYGRTYEEGK
                     KIGWKDGVRAIWCILKYGFFRM"
     gene            complement(7765..8208)
                     /locus_tag="PK742_01070"
     CDS             complement(7765..8208)
                     /locus_tag="PK742_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQU75207.1"
                     /translation="MSFVTIGQNASDLNSLSVNDEAAVKAAVDSFLVALGRGDVERVK
                     ALFLPNANIGSVVMREGVTKIYTSSFEDFIAKREAGRNFEEPVRTYTVNISQGLLAFV
                     RADATVVIDDIPDHFTDDFFILMKEDGEWKFLSGSYTSYPLEETE"
     gene            complement(8283..8756)
                     /locus_tag="PK742_01075"
     CDS             complement(8283..8756)
                     /locus_tag="PK742_01075"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQU75208.1"
                     /translation="MEEQPLVDKEYLLEKFPGKGGWTYAMIPEVLQDPHAPFGWVQVR
                     GRIDSYTFDHYKLMPMGNGHLFLPVKKSVRKLLKKEAGDTVHVILYADDTPVEITQEL
                     LDCFALEDPKVLKAFRSLSDAEQKRFLEHIYSAKTDDVKTNRIASMISSLTTKSY"
     gene            complement(8763..9734)
                     /locus_tag="PK742_01080"
     CDS             complement(8763..9734)
                     /locus_tag="PK742_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQU75209.1"
                     /translation="MSKLDQIKAPIAAELDIFEKKFREAMRSQVPLLDRVMYYIVQRK
                     GKQVRPMFVLLSARLHGEIGESTYTAASLIELLHTATLVHDDVVDDALERRGFFSVNA
                     LWKNKIAVLVGDYLLAKGLLLSLDRREHRMLQITSNAVKEMSEGELLQMEKARKLDIR
                     EEIYYDIIRKKTASLIAAACSAGAASVSSDEDLIEQMRTFGELIGIAFQIKDDLLDYG
                     DADIGKPTGIDIKERKMTLPLIYALQQAEKKDKRHIIHTVRTNNNDPRKIREVIDFVK
                     QAGGIDFAEKEMLRYREDAFALLDTMPEGESREALRKLVIFTTDRKK"
     gene            9774..10610
                     /locus_tag="PK742_01085"
     CDS             9774..10610
                     /locus_tag="PK742_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQU75210.1"
                     /translation="MKRYDDRVDGLHGRPITFDIRVPDGEGSFPLVIFAHGFKGFKDW
                     GHFNLMADWFAGQNFVFCKFNFSHNGIPALGGQELTDLDAFAANNFSIELDDLQILTD
                     HLISRQQLYQIDTANISLIGHSRVGGLVMLRATEDARISKVVSWAGVTDLGRYWMGDL
                     LDNWLRDGVQYVRNSRTGQMLPMDIQFYHDYIRHKERLDLPARMHQLHQPLLHIHGTA
                     DEAVPVSWAQELKLRHPRTHLHIMDGADHVFGSRHPWTEKTFARGVQEVLETTLAFLK
                     ME"
     gene            10644..11429
                     /locus_tag="PK742_01090"
     CDS             10644..11429
                     /locus_tag="PK742_01090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQU75211.1"
                     /translation="MTNKIITAFSVALLLTACSQGGGINIFSVEDDKALGAQIEAEIA
                     ADPATYPVLSEASYPEAYALINDIRDRILASGEVYYKDEFVWNVKIIKDDNVLNAFCT
                     PGGYIYVYTGLIKYLDNEAQLAGVLGHEIAHADLRHVTDQLTEQYGVSLLLSLLLGQN
                     GQVLGDIAFGLSGLAFSRADESQADEYSVIYLCPTEYAAYGAAGFFEKIEEDGGVGIP
                     EFLSTHPDPENRIEDIYAKYDELGCGGTGTFEDRYQDLIDALP"
     gene            complement(11471..11992)
                     /locus_tag="PK742_01095"
     CDS             complement(11471..11992)
                     /locus_tag="PK742_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU75212.1"
                     /translation="MRSILLICLLLVSGAGMRAQIPELGVSKRMVDTCTINFDHPEAG
                     DYIRLYARNFLDDSARGVVSPAFYDTLFMILEQLPDCTFEVASHTTSDGPAARNYKMS
                     LRRSSFFVHYLVTYCGMKKERLQAVGYGENCPLIQCTDGETCTPEQKEKNQRLEIMIL
                     STGNEEQKTAQPD"
     gene            complement(11992..14565)
                     /locus_tag="PK742_01100"
     CDS             complement(11992..14565)
                     /locus_tag="PK742_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75213.1"
                     /translation="MQKKIAFAIVLVTLALTLAVMVYFMYFVHPQPDMPAPTDRPLKQ
                     EEGAEHNDAREAWIEQMHRTAPGANWRQMDRELRLQLAASRSEQYALGGTRADVWDTL
                     AAGYAVGKWNEVGSFNTAGRVRATEIDFQTGTVYAFSQGGNLWKGDLEGDNWTVINDN
                     FNVQSAIFLQKVGDELVLVTNEWAVQSVYRTPDEGLSWTACAGLENMTAWGSILEADM
                     LNNEERTLYILVQEWDYVDWGAVTALYRSHDLGSTFEFVANWREDVYGNTENFSMWAP
                     RYGDDRLYVNAGNLLYTVDGADGDTTYLAEIPVTTNGTHMLSGVQNTDGTSLYLATAQ
                     YGDGTQIYRSSDAGLSWTESGLVESGAFSRGSFHSSQLETGYLWFGGVNTFRSFNGGD
                     TWTLVNEWWEYYGNEASKLHADIPFIMPFLDTISNEETLLISTDGGLYASDNYGLTNT
                     NITMEGMRNAQYYDLYTYRLVTDVIFAGAQDQGFQRSFSSVDDQYYFDQLISGDYGHL
                     VSSDGGDHLWTVYPGFAMMINDASGPANMFFGDFVGSGYLWLPPIMEDPDDPEVCWWG
                     GGDHLYKLKKNFSGITYEQYDYDFDVGLGGTAIAAIAYSPIDHDHWYVMMSDGKFFHS
                     DDRGITWTKTIGFDGPDSHYFYGSSIVPSTTELGKVYIGGSGYSNPPVWVSEDHGANF
                     VSMSFGLPSTLVYDMAILPGDSMLFAATEVAPYVYIPETDTWYDLSGLDAPYQTYWAV
                     EYVEEIKTVRFGTYGRGIFEWKLYEEEPPLSISGITSGSLQIFPNPAIDHIAVSIPDH
                     IPNATITLYDRKGQAIWNTRAALNRNIPYYLSLPGLPTGMYFATVSGNNKTYTSKLII
                     N"
     gene            complement(14616..14981)
                     /gene="crcB"
                     /locus_tag="PK742_01105"
     CDS             complement(14616..14981)
                     /gene="crcB"
                     /locus_tag="PK742_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HQU75214.1"
                     /translation="MWSLVLIGLGGAAGAVLRYLVNLWSLTWYAGPFPLGTLLVNVAG
                     SLLIGIGWSTIGNDTTRLLLLTGLLGGFTTFSSFSLETVRLWEDGQMTLALWNILLNN
                     LSAILACMAGYWIGKMLHA"
     gene            complement(15005..16627)
                     /locus_tag="PK742_01110"
     CDS             complement(15005..16627)
                     /locus_tag="PK742_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQU75215.1"
                     /translation="MKNFILFVLAWSMLLSVHAQTESKNPNPKLIVGVVVDQMSYDLL
                     YRFWNNYSEGGFRRLVKNGYSCENTHYNYVPTYTGPGHASIYTGSVPAVHGIVGNNWF
                     DPVHNNEWYCTKDTLVSGVGTITAAGQMSPAPLLTTTITDQLKVASNGRAKVVGVAMK
                     DRGAILPAGHAADAAYWFDGYTNAFVSSTWYMETLPAWVEQFNKQQWANQYMATDWDL
                     FLPKNSYTNSTADNVPWETLREGETAPVFPHMTSNASSMELIKGTPYGNSLTVDFAKA
                     ALLGEGLGKDQQTDFLCVSFSSTDYVGHAYGPYSMETEDTYIRLDRDLAAFLQFLDTE
                     VGSGNYLFFLTADHGVAPTPGYMQSLEIPAGVFSELSMITQMDSVLDAAIGTADWILS
                     FENQQVYLNPDAVQQNQTYTGHVADVLRNWAFTSANGVAHVLEVDALGYSNIPSQYKE
                     MYINGIYPKRCGDILFVFEPNWFGYSNTGSSHGSQYAYDTQVPLLWYGWKVRNGKSWT
                     RYAITDIAPTIAAMLRIPQPSGCIGQVIEEMK"
     gene            16791..19385
                     /locus_tag="PK742_01115"
     CDS             16791..19385
                     /locus_tag="PK742_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU75216.1"
                     /translation="MAQQTTVSGRVINENNRQGLAFVNVSLDDTHGTFTNENGQFRIE
                     DVSPGTYVLRISCIGFDPYQSASFEVQPGQPLDLDEISLTEKTIQIEEITITEQYKVW
                     DTRYTGTNNVISAKKLERFQPIGTEEMIRTVPGVNIAGDMGISNRPNISIRGSDPRRS
                     NKILLLEDGSPISPAPYLAPGAYYNPPADRLDGIQIIKGPDALMYGANSIFGVVNYIT
                     RRPVNDPGLRLRVQGGQRGYMDASASYGGTWGNSGAELMALYKRFNGYTDNADLHMMN
                     VSGKWFSELSDRQSLYTKIVIQKEYVNNSLSGMTPFTFDTDPTRHPFEADEFTSHRYG
                     LDVVHNFKVNEKLSLQSKVYGSDFYRDWWKQNSVVVHAADVQEYVGEENYQQFYSYLD
                     GLEFTDDDYVRVGKRVNGIESNSDSRWQYLVYGAHEKANLKWAAGNNLEAMVKVHGET
                     YHDITILGDSSKWARSGRTSADAFYTVLATSGYVRNDFRFGRFSVIPILRYEQIHLTK
                     NDLLQNGSDPANTGHDFGEITNDFGEFTPGLSLVFRDINSGSNNWEFFTGVYKAFSSP
                     TTAISFNELLNGEVVPSPDIANLQPEESWNQELGARWINNRNSMNGQLSLFNMFISNF
                     YSPARAQAFQTLGAVRISGAELAASYNILKSLGVHDQALTLSGAFTYMQSAITAGTLS
                     DADLLTKVVHSSATRQELIDKINADRSAFEVYLDGELYEGAMDISQFGQITSVAITYG
                     DGGVTDYAVPYVTPVIFNVGLNYESKHWMINSVVNHVGPQFTEFFNFENESADGSIGE
                     LPAYTTLDANLAYELGDRGQAKDIRFFVSGKNLTNQIYRSSRLNRATGGLFPAGFLQI
                     NAGVSLSL"
     gene            complement(19392..20219)
                     /locus_tag="PK742_01120"
     CDS             complement(19392..20219)
                     /locus_tag="PK742_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU75217.1"
                     /translation="MLKIIAATDFSEAAKHALRFIVNATRNMDREILVLNVVHLDGPP
                     PSAMMSSIIESLKTKAYFDYQTLSAEISAEFGEEIPMRFHAEVGHPASRIIQRYAAAE
                     GADLIVMGMKGLSGVEKAFLGSVAANVAMHSRFPVMIVPADCQIYPIEHIVDATDLYQ
                     DEIEFGAVMQFARLFGAEVSMLHVFASDDAVEQTNIQALNEGLVKKYGELKDVRYVSV
                     KNDNVVNGIEAYTNEHPTDLLVVFTHKRLFYERLFDRSISRELAFESKVALLVLKSE"
     gene            complement(20328..21902)
                     /locus_tag="PK742_01125"
     CDS             complement(20328..21902)
                     /locus_tag="PK742_01125"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQU75218.1"
                     /translation="MKRLLLSLSILGIWIPAQAQLVVWEEPNDTTFIFSLAGPGVTVS
                     NIERNCAEGASGFYNSLDANVGIDSGIALTSGSLINAVGPNNLGSASVANGYDGDDDL
                     DALLPGYLTYDACYIEFDMTVAADTVRIDYVFGSEEYLEFVGTSFNDVFAFWVSGPGI
                     PDPVNIATIPGTDLPVAINNVNSTSYPEYYVVNGDGSSDPYASDSSYIQYDGLTVVLT
                     GEIAVTAGETYHMKIAVADAGDMIFDTGVFMRTGSLGSLRMGTGYYGDGEALVAAEEC
                     SNGYIEFTNFVPSDLDLVIDYTIDGTAEMGVDYEVIAEQITIPAGMSTAILPIVPISD
                     ELNEGDETIILHLFNPQSGYVYDEVEVILADRIDADFSTSTTDATISFTDNSDPAVEW
                     FWDFGDGTTSTEQNPVHTYAAQGSFEVCLTVTNENSCASTACKTVSAATDLNDAASGW
                     LSVYPNPASGSFTVSPGGSDVYSLSVIDLSGRIVATYQELLGQQIIDCSQWAAGTYML
                     RLDTASGTHLEQIDIR"
     gene            21970..22683
                     /locus_tag="PK742_01130"
     CDS             21970..22683
                     /locus_tag="PK742_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQU75219.1"
                     /translation="MQEKAQVFRMKKFCIAQQHAAMKVGTDGVLLGAWAEGEGVRSIL
                     DIGTGSGLIALMLAQRFPVAEVHAIELDVHAAEQALYNAAASPFSSRIQVFQASFQEW
                     ISAVVHTYDLVVCNPPFFSGGWEVPDVKRRMARDATFLPVEVLFSGAEKLLSAGGAFT
                     LILPAEVHQVYARHAIHNGWYLCRQTNVYSRAGKPVNRILSAWYRQPQPIRTDEIILE
                     GPDGTRHSSYQRLTEAFYL"
     gene            complement(22745..23176)
                     /locus_tag="PK742_01135"
     CDS             complement(22745..23176)
                     /locus_tag="PK742_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75220.1"
                     /translation="MPKKKPMEGKPEVHEELEGFDMQINEFGEIISNTSVDKLNRFLN
                     KHVEDKKFKGMDQLPYAQHDDDDDEDLDDDDFEEGDDDLDFDSDDDDEIADLVKNMEK
                     DFGKKGGKKGKDFEDFDDEEMIDEYGEEYGEEDDYDFDDDL"
     gene            complement(23186..24727)
                     /gene="gltX"
                     /locus_tag="PK742_01140"
     CDS             complement(23186..24727)
                     /gene="gltX"
                     /locus_tag="PK742_01140"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQU75221.1"
                     /translation="MENAGKVRLRYAPSPTGPQHIGGIRTALFNYLFVKKHGGTFVFR
                     SEDTDQTRFVPGAEEYIVDSLQWAGITIDEGIHVGGPYGPYRQSERKDMYRQYAEQLV
                     KDGHAYYAFDTPAELEEMRERMSSQGNPSPAYDHITRQYMKNSLSLSQDETEKRLASG
                     DAYVIRLKMPRNEELKFEDLIRGWVVFNTSQLDDKVLFKSDGMPTYHLANVVDDHLMH
                     ITHVVRGEEWLPSAPTHIMLYRSLGWEDTMPRFAHLPLVLKPEGNGKLSKRDGDRLGF
                     PIYTLNWENPLTGEKISGLAERGFLPEAYMNFLALLGWHPGGDKEVMTPEELVESFSL
                     DRVGKSGARFDPEKAKWINQQHIKMKTGAELAELLAPTLEAAGVSAEPAFVAAACELM
                     KDRAVFIPDLYEQGKFLFGDDLEYDEKTVAKKWKKENAGLFENLRERLFLLEQFSESE
                     LERTVHDFMEDKGLKAGEVMPVLRLALAGGLQGPPVSAMMALLGKESCDNRLKKGFQL
                     FNTLI"
     gene            24776..25387
                     /locus_tag="PK742_01145"
     CDS             24776..25387
                     /locus_tag="PK742_01145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQU75222.1"
                     /translation="MYFTRTPAYLKSLFGGLTWDVPETDAIFLTFDDGPVPGVTDKVL
                     DMLAASEVKATFFCIGHNVQKHPELYRRILDEGHAVGNHTFHHLNGWRSNDETYLEDV
                     EKCADLVSSRLFRPPYGRIGYRQMQLLKQQYRIIMWDVLSGDFDELLTADDCANNVLS
                     GMRGGSIIVWHDSEKAAPRMLPALERVLHTIRIRTLRTETLRG"
     gene            25384..26163
                     /locus_tag="PK742_01150"
     CDS             25384..26163
                     /locus_tag="PK742_01150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQU75223.1"
                     /translation="MKLIDSHAHIYVADWGEDQQAMILRAMDAGVEAVIMPHIDEETS
                     EVLLQLYQNYPDWCWPAMGLHPCSVKDDFSELLDDYEKRLANNSIPWKGIGETGLDYY
                     WDLTYKKEQQKAFDRQLSWACELDLPIIIHSRDSLDDCISMAKQRQDGRLKGVFHCFS
                     GNREQVQQINDLGMYIGIGGVATFKNGGLDKVLLPEDLDLMVLETDSPYLAPAPHRGK
                     RNEPAYLPLVVKRLSEILDLTEEEIARTTTANSSKLFSLPA"
     gene            26207..26294
                     /locus_tag="PK742_01155"
     tRNA            26207..26294
                     /locus_tag="PK742_01155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26241..26243,aa:Ser,seq:gga)
BASE COUNT         6740 a         6424 c         6586 g         6803 t
ORIGIN      
        1 agccgccggt atgatcatca aataaattac ttatgcataa taaccagccg ctgcatcatg
       61 atgtagcggc ttttttgttg ttgtaacttt aactgtatga agcagatcct gctctttctg
      121 ttgctggccg gtcagtttgt acaggccctg acccaggtga atgcacccaa tagaaccgat
      181 aaggaaggtt tgcgcacagg cgaatgggtc atctatttcc aacaggatga ccaggtgacc
      241 gacaacccct ccggagccac acgatactac ctggtcaatt ataaaaaggg agaaccgaaa
      301 ggattcacac gtgaatatgc catcgatggc accctcgtct gggagggcga actgcgttcc
      361 gtgttgcccg ataccataca cggcgtgagc cgtggtttct tcagagacgg aactgtgagc
      421 tatgaataca cgtttgctga tggcctgatg cagggtccgg ccaaacagtt ctatgagaac
      481 ggagaactga aatggagttg taactataca gacgattttc cggacggcac ctataaagag
      541 tattacgaca acggaaatct catgcgggaa ggtccggtgg tgaaggggaa aaagaacggt
      601 tacttccgat tttatttcat tgatggcaat atgcagaaag gtgtcaccta tgtgaatgac
      661 gtcatggatg gcccctacga tgaatatgac aatggcaaac tgaagtacca ctatgcgatg
      721 gcgaatggtt tgcgggaagg actcgtgacc cagtatgatg ccagtggtca ggaagagaac
      781 tactatatgt tccatgcaga ttcagttatc ggtataaaag aggtcatcgg cattgtaagc
      841 agcaatcttt tgcctggtat ggatcagcgc ctcgggttga acatgtgcct gctgatggaa
      901 gaatatttca ggaagaaata cggcgaagat tcccctctct atcctttcgc agccagcagc
      961 ctggcacagt attatttcac tcttggcgat gaacagaacg gtcatcactg gaccatcaga
     1021 agctatgaac tggagaaggc gcagatgggc acagaccagg aagcctcttc agacaaatgg
     1081 catttgcttg gcctaatgct ggaatcctat gctgaatcag ataaggcatg ggaatgttat
     1141 cgctatgcca ttcgtggaag ttacgagaaa ggtaaaccca cagagactac agtaagctat
     1201 atgtcgcggg cagcatccat ggcagtgaac cttggccatg agcaagccgg ctttgaacaa
     1261 tttgatgccc ttttgcttac ctgcgataca ctcggtaaag acggagcagt ggtctgcaca
     1321 gatgcagccc tgaattatat ttccttcctc tccgcagctt tccgcgatca ggatgcgttg
     1381 catatcctgg agtcaagaag aaagatagca aaagaagctg gatctctgaa tgcctgggac
     1441 ctggctaccg ccgatgtcat catgcagctc aaccgcgaag atgaagccat ggatatctat
     1501 ggtcgtttgt ataacaattt tactgcagac cgtgatactg cacagcatat cgagatcgca
     1561 tcaagcatgg cagaatacca gatgcagtca ggtaactatg cagctgcaga gaagctctgg
     1621 ctggaaaccg ttgcgctgtc agcagccata tcagatgaag acagtctgga ttattacagc
     1681 aggctggatg atctggccac ctactatgat cgggtgggaa gaaagtttca ggccatacaa
     1741 tacagccagc aggtatttgg ctttcgcctg cgcatggcga aggataccac cagtatggaa
     1801 tacatcttct ttggcgatgc tctgaccaga gaactggctg tgaacagcgc actatcactg
     1861 ggtcgtttat acaccaatgt gggcatgtac gatgaagccg gatccatgtt cacttatgcc
     1921 ctggatgaat cactggccct gcatgctgat acatctttcg aatatgccta tgcactggaa
     1981 gccatggcgg gttatcagat ggataaggaa gactttgatg cgtctgaaca ttcctatctt
     2041 gaagccatac ggttgtctga tacctattat gcagacatca atgtgaacgg caccacctgg
     2101 aaagacaact tgtctgaatt atatatgcgc atgcatcagc cggagaaagc gctggttgtg
     2161 gcagagcagg tgctgtctga taggcaacag caatacccgg aaggacatcc cattctcacg
     2221 gccagctaca gtcgcctgtc aggcatactg ggtgccctgg gtcaaacaga ttctgctctc
     2281 actttatatg aaaaagtact ggatgcagaa gtggcccggc tgaatgaaac ttttgcagtg
     2341 ttgtcggcag ctgagcagga agactatctg accactttcc gttttgattt tgacatctac
     2401 aacgacatgg tagttcggta tgcgaagccg ggcagcctgc actttcagcg aatgctcaat
     2461 tatcagttac tgaacagatc actattgttg tacagcggta ctgctgcacg aagaagcatg
     2521 gagcaacatc cggatgcagc catacgaagc atgtatcagg aatggatgga aaccggattg
     2581 tatctgagtg gtctggcggg cagggagact gatcaggagg tgccgtcttc gctgatcgat
     2641 tccttgcagc aacgcactga acaattggag aaggatctca ccctgcgcac aggtcaggac
     2701 atcacatttg tgccatctgt aactgcccat gcattaagca gcgctttgca ggaaggcgaa
     2761 tgttatgtgc tctttctgcg ttatccggtc tttgaagaag cacatgtaaa gcattgggaa
     2821 cttggcgcac tgatcctgaa tgataccggc aacacaatgt tcgtttctat tggaaaagaa
     2881 gcaacactgg agaagttact ggagaacaaa gagggagaat ctgtacagga ccgcatcagc
     2941 agactctaca cgtttcctga actagatgaa gacagcctgc tctttcaggg cgatcgtgtc
     3001 tttgccacta cctggaagcc attgatgcct tttgtgcagc atagtaagag agtctattac
     3061 acgccggcgg gtatcatgaa tctggtcaac ctgagtgccg tacctgacaa actggatcat
     3121 tttttatttc agcagatgga actcattcag ctggcaggcc cggaagctgt cgtgcaacgg
     3181 gccacagaaa gcacattgaa aagcaatgcc accctcacga tgataggtgg ggtgcagtat
     3241 gatgccagcg atgatgcttt gcgctctgcg gtgaaaggtg tggaaacagc atcaacagat
     3301 attccactgt cggccgaaag tagtgtcatc acccgtggcg acaactggaa ataccttccc
     3361 ggtaccctcg ctgaagtgca gggcattgac cggcagatca aagaaacaaa gtggaaggtg
     3421 gaagaagtga ccggtaccct tgccaccgaa gaactggttc gtggtttcag cggcgatcat
     3481 gctccggata tcctccatat agccacacac ggattcttcc tggacaacag ccagggtgat
     3541 cctttacatc gctccggtat cctgctggcc ggaggcaaca gaagctggca gggcgacagg
     3601 ccttcagggg gattgcagga cggcatcttt accgcagcag aggtcagcta cctcgatctg
     3661 cataaaacga aactggtcgt actcagtgcc tgcgaaacag gtctgggtgc ggtggatgca
     3721 aacgaggggg tatttggact acagcgtggc tttgcgctgg caggggtgga ccacaccatc
     3781 atgagtctat ggaaggtgag tgacaaggaa accgccctct tcatgcagac cttctacggc
     3841 cacctggctg caggactttc tatacaggaa gcttttgcac gcacccgaga ttccatgagc
     3901 aaacgctttg ctccatacta ttgggctgcg ttcgttttgc agcaataaat cagaagctgt
     3961 gtatgctgca tatgccgggt tgaaagtgca ctcagtaccg tcatattttt gtcaaccgca
     4021 atatatttct aagtttttag ttgtataact aaacaattag ttatacgttt gccttatgaa
     4081 agagctgacc aaagcagaag agcaggtgat gcagatactc tgggaactgg agaaaggatt
     4141 cattcgtgat atcctggaac gcatgcccga tccgacacct gcttatacaa cagtatctac
     4201 catcgtgcgc atacttgaga ccaagggatt tgtaggtcat cattcatatg ggaagaccca
     4261 cgaatatttt cctctggtaa gcagggaaga ataccgcaag tttatcaccg gtggattgct
     4321 tcatgattat ttcggtggat cggtcagcaa tctggtctcc cattttgcgc aaaaggaaga
     4381 tctttctgcg aaagagctcg atgagatcct tcgcgttgtg cagcgatcca aaaaacaaaa
     4441 gccatgagta ccgaagtatt attcatcaaa gtacagctgg ccatgctcgt gctgtacgga
     4501 ttctacaaac tgctgttctc tgcatccgta catcatcagg tgaaacgttt ttacctgctg
     4561 gccatcatac cggtagcgtt gctgcaccct tattttttcc gtggcagtgc cggtggcgaa
     4621 accctgggcc gtgtgcaggt gctggatact ttcatcgtgc agaccgttga ccagagcatg
     4681 aacacacttg accgcaacat cacctgggga atagcagaat gggtctatct gctgataact
     4741 gcggccttgt ttctggtgct gatgatacgg gtagtgcgat tattatggat ggcctggcga
     4801 ttgccgaaag aagtggtggg caacaagcga atcatccgca tacccgaaga tcattcacct
     4861 gccagtttct tctcacttgt ttttatgccg gaaggattat ccatagacac cggaaaggtc
     4921 atcctggatc atgaaatggt gcatgtgagg caactgcatt ctgttgatgt attgttatac
     4981 gagtttgtac gttgcctgtg ctggttcaat cctgttgcct ggctgctgga gcgggagatc
     5041 aggctcgtgc atgagtacat tgccgatgaa caggcaggaa aagatgcacc gcatttttac
     5101 cgcgacactc tgctggcata ccgactgggt attgagccgg cattactgac caataatttt
     5161 taccaatcat ccaccttaaa aaagcgaatc atgatgttac aagaatcatc ttcctggaaa
     5221 acaactgtta tccgtcttgc attactgctg cctttactgg gtatgttcag cctcatcaat
     5281 acacctgcgg cagcgcaaac ctccactgat aaagtatatc agcaggtgga gcagatgcct
     5341 gaatttcccg gaggaatgga cggcctgatc aagtacctct ccgaacaggt tgtgtatccg
     5401 gagtctgcca aaaaagatgg cgtgttcgga cgggtgatgg tgcaattcgt tcttgacgct
     5461 gaagggcatg tgacccaggc tcaggtagtc aaaggggcag ccaaaccact tgatgaagaa
     5521 gcgctgcggg tggtgaatgc catgcctgac tggcggccgg gtatgcagga tggaaaagcc
     5581 gtgagtgtgc agatggtgtt gcctatccag ttcagcctgg atgaacctgt caaaaagtaa
     5641 tgactacttt ctcaggcgat accccatcgg ataatagttg ttgatccgat ggggtatttt
     5701 tttgacccac cccagtatgg caccgttgaa accaacaaga gaccatcctg attcaaacaa
     5761 agcgggatca agttcttccc tgcgcagata tttcaatgcc tgtttctcat cgaggtagat
     5821 gaccggtatt tcgtcatgat acaatccgct catggcgagt ccgtgtccgg gcaacagatc
     5881 tttgcctttc tgtctgccag ccagcacacc ggcctgcaca acaggaaccg cagcacatat
     5941 ttgcagccag tcatgttccc tttgcaacgg ccatcgccag gtatcctcat cccagctgta
     6001 gaaacaacca tcggcacgac cggtcatgtc tgtccaggcc tgctcatcat aagctgttga
     6061 ccgttcagga agtttttgcg gacgaaatga tcggtcagca ggactgtttt cgcgaagccg
     6121 catcaccgcc aggaagaatc cttccccctg caacctgtcg ggatagaacc gaaagcatcc
     6181 ttcctccggt ccgcagtgca gaatattcca ttctgccggt acctccagat gcactatttc
     6241 caaatgatgg tctttcagta agcgactgat caccgcttcg ttttcttctt ccgaaaaact
     6301 gcaggtggaa tacaccagca ctccacctgt ttgcaatgcg ggtaagacat catcaagaat
     6361 cctgttctgt cttgctgcac aaagcgccac attgtcggga ttccattcat ccatggcatc
     6421 ggcatctttc cggaacaatc ctgatccgct gcatggggca tccaccacga tgaggtcaaa
     6481 gaacgacggc aggcccgcaa acgcacttgc atctgcagag ctgaccacca cctgttcatg
     6541 tccccacttt gtgatattct ctctgagggt acctactctg gaacggatca cttcattgct
     6601 caccagcata ctgcccgatg gtaaagcatc acacaacagc gttgattttc ctccgggaga
     6661 ggcgcatagg tcaagggccc ggacgggttc atgggtaaac aattgcttca ctacatggtc
     6721 cagaaacata gagctggctt cctgcacata gtaggcacct gcatgccagt gcggatccaa
     6781 tgcgaaaaca ggacgctccg gaagatagat gccgtgtgta caccagggca ccagcggcag
     6841 accatggaag cgtataacag gcttgcgctg attgatgcgc acagatacca ccgcctcctg
     6901 ctcaagcgac tgcaacagtt gttcaaactg cacacccggt tgttgctgca ggtgttgttc
     6961 aaatccgtca ggccagtggt tcatgcgtta aaaatacata ttacagcaag ggggcgtatc
     7021 tttagcctgt gaatttcagc aagttgagcg tactggtacc ggcttataat gaggaggcca
     7081 ccatcgtacg gattctggat accttacaat ccgtgcaact tccagaaggc atcctgatgg
     7141 agttggtagt agtgaatgac tgttccactg accgcaccgg ccagatcgtg gaacagtata
     7201 tcgcacagca cccggatgca agtatcttgt atgagaagca tgcaaaaaat gcaggtaagg
     7261 gtgcggcgct tcatactgcc attcaacttg ctaccggcga tctgatcgtg gtgcaggatg
     7321 ctgacctgga atatgatccg catgagttca gcatattgct caagcctttt ttggccggac
     7381 aggcagatgt ggtctatgga tcccgtttca tgggcggaaa tccgcaccgc atattgttct
     7441 tctggcacag cataggcaac agattcctta cattcttcag caatatgttc tccaacctca
     7501 acctgaccga tatggagacc tgctacaaga tggtacgtgc agacattctg aagagtcttg
     7561 tactgaaaga aaagcgcttt ggcttcgagc cggaggtcac ccagaaactg gcacgtatca
     7621 aaggtatccg catctatgaa gtaggcattt cctattacgg ccgcacctat gaagaaggaa
     7681 agaagatcgg ctggaaagac ggcgtacggg ccatctggtg tattctgaaa tatgggtttt
     7741 tcaggatgtg aagcctctcc ctgtttattc agtttcttcc aacggatagg acgtataaga
     7801 accactcaga aatttccatt cgccatcttc cttcatcaga atgaaaaaat catcggtaaa
     7861 gtgatcgggt atatcatcta ttacgactgt tgcatctgcc ctcacaaacg ccagcaagcc
     7921 ctgtgaaatg ttgaccgtgt aagtccgcac cggctcttca aaatttctgc ctgcttcccg
     7981 cttcgcaata aagtcttcaa agcttgaagt atagatcttt gttacacctt cccgcataac
     8041 cactgagcca atgttggcat tgggcaggaa cagggctttc acacgttcca catcccctct
     8101 tcccagtgca accagaaagc tgtcaacagc tgctttgaca gcagcttcat cattgacaga
     8161 cagtgaattt aagtcagatg cattttgacc aatggttacg aatgacatga agcatgttaa
     8221 cagtattacc attgccgccc tgttgatacc ggttctttcc atgtattatt cagttaatgg
     8281 gttcagtatg atttggtagt taaagatgat atcatggatg ctattctgtt tgttttgaca
     8341 tcatctgttt tcgcactgta gatatgttca agaaagcgct tttgttcagc atcgcttaaa
     8401 gaacgaaaag ccttcagcac tttcggatct tccaatgcaa agcaatccag cagttcctgt
     8461 gtgatctcca caggcgtatc atcagcatac aggatgacat gaactgtatc gccggcttct
     8521 tttttcagca gtttgcgtac agatttttta actggcagga acaggtgtcc attgcccatt
     8581 ggcatgagct tataatggtc gaatgtatag gaatcaatac ggcctcttac ctgcacccag
     8641 ccaaaaggtg catgcggatc ctgcaacact tccgggatca ttgcataggt ccagccgcct
     8701 ttgccgggaa atttctccag caggtattct ttgtcaacca atggctgttc ttccatgaca
     8761 gcttatttct ttctgtctgt agtaaatatg accagtttgc gcagcgcttc ccggctttct
     8821 ccttccggca tggtgtcaag aagggcaaat gcatcttccc ggtaacgaag catctctttt
     8881 tcagcgaagt ctataccacc ggcctgcttc acgaagtcta tcacctcccg gatctttcgc
     8941 gggtcgttgt tgtttgtacg aacagtatgg atgatatgac gcttgtcttt tttctctgcc
     9001 tgctgcagcg catagatgag tggcagggtc atttttcttt ccttgatgtc aataccggtg
     9061 ggtttcccga tatcggcatc accataatcc agcagatcat ccttgatctg gaaagcgata
     9121 cctatcagtt caccaaatgt gcgcatctgt tctatcaggt cttcatcact actcacactg
     9181 gcagcacctg cactgcaggc agcagcgatc aggctggccg tctttttccg gatgatgtcg
     9241 taatagatct cttcgcggat gtccagcttt cgggctttct ccatctgcaa gagttctcct
     9301 tcactcattt ccttcacggc attggaagtg atctgtaaca tgcgatgttc tctgcgatcg
     9361 agagacagga gtaaaccttt cgccagaagg tagtcgccca caagtacggc gatcttgttc
     9421 ttccataagg cgttgacaga aaaaaatccc ctgcgttcca gcgcatcatc caccacatca
     9481 tcatgtacca gggtggcggt atgcagcaat tcgatcaggg aggctgctgt ataggttgat
     9541 tcacctattt ctccgtgcag tcttgcactc agcagcacga acatgggcct tacctgcttg
     9601 cctttccgtt gtacgatgta gtacatgaca cgatccagca gtggcacctg gctgcgcatg
     9661 gcctctctga attttttttc aaaaatatcc agttcggcag cgatcggcgc tttgatttgg
     9721 tctagttttg acaccggttg caagttacgc tgcaagccgt aaatcaccta aatatgaaga
     9781 gatacgatga ccgggtggat ggccttcacg gtcgcccgat cacctttgat atacgggttc
     9841 cggatggaga aggttccttt cctttggtga tctttgccca tggcttcaaa ggtttcaaag
     9901 actggggaca tttcaacctc atggccgact ggtttgccgg tcagaacttc gttttctgta
     9961 aattcaattt ttcacacaac gggattccgg ccttaggcgg acaggaactg actgatctgg
    10021 atgccttcgc agcgaacaat ttttccattg aactggatga tctgcagata ctgaccgatc
    10081 acctgatcag cagacaacaa ttgtatcaga tcgatacagc aaatatcagc ctgataggac
    10141 acagccgcgt tggcggactc gttatgctgc gggccacaga agatgcccgg ataagtaaag
    10201 tggtttcctg ggccggggta acagatctgg gcaggtactg gatgggagat ctgctcgaca
    10261 actggctgcg agatggggtg cagtatgtgc gcaacagccg cacaggacag atgctgccaa
    10321 tggacattca gttttaccat gattatatca gacacaaaga aagactggac cttcctgcca
    10381 ggatgcacca gttacatcaa cctttgttgc acatacatgg cactgccgac gaagctgtac
    10441 cggtttcctg ggcacaggaa ctcaaacttc ggcatccaag aacacatctg catatcatgg
    10501 atggtgctga tcatgttttt ggaagcaggc atccctggac agagaaaact tttgcccggg
    10561 gtgttcagga agtgcttgaa accacgctgg catttttaaa aatggaatga aatttggact
    10621 ttcgtagctg ctaacctact gacatgacca ataagatcat cactgctttc tccgtggcac
    10681 tgttgctgac cgcctgttct cagggcggcg gtatcaatat attcagtgtg gaagatgata
    10741 aagccctggg ggcacagata gaagcagaga tcgctgctga cccagccacc tatcctgttc
    10801 tgagtgaagc atcctatcct gaagcctatg cacttatcaa tgatattcgc gacaggatcc
    10861 tggcaagtgg tgaagtctat tacaaagatg agtttgtctg gaacgtcaag atcattaaag
    10921 atgataatgt gctcaatgcc ttctgtacac cgggaggtta catctacgtc tatacagggc
    10981 tgatcaagta tctggataat gaagctcagc tggcaggtgt gctggggcat gagatagccc
    11041 atgcagacct gcgtcatgtg accgatcagc tgacagagca atatggagtt tctctgctgc
    11101 tgagtttgct gcttggacag aatgggcagg tgttgggtga tattgccttc ggcttgtccg
    11161 gtcttgcttt cagtcgtgcc gatgaatcgc aggctgatga gtactctgtc atttatttat
    11221 gtcctactga atatgctgca tatggtgctg ccggcttctt tgaaaagata gaagaagatg
    11281 gtggtgtggg tatcccggaa tttctcagta cccatccgga tcctgagaac aggatcgaag
    11341 atatctatgc caagtacgat gagctgggtt gcggtggtac cggaactttc gaagaccgct
    11401 accaggacct gatcgatgca cttccatgat cagtataagc ggcagaaaga gaacagattt
    11461 ctgagaaagt tcagtccggt tgggctgttt tctgttcttc attacctgta ctcaggatca
    11521 tgatctccag cctttgattt ttttcttttt gttccggtgt gcaggtttct ccgtctgtac
    11581 attggatgag cgggcagttt tctccgtagc ccactgcctg cagccgttcc tttttcattc
    11641 cgcaataggt aaccagataa tgcacgaaga atgaagagcg gcgcagagac atcttgtagt
    11701 ttctggcggc cgggccatca gaggtggtat gtgaggcaac ctcgaaggtg cagtcaggca
    11761 gctgctccag tatcataaaa agtgtgtcat agaaagcagg agaaaccaca cctctggcac
    11821 tgtcatccag aaaatttctg gcatacagcc tgatataatc tcctgcttcc ggatggtcaa
    11881 aattaatggt gcaggtgtcc accatgcgtt tgctgacgcc cagttcaggt atctgtgccc
    11941 gcattccggc accagaaaca aggagcaaac agatcagcag tatggaacgc atcagttgat
    12001 gatcagttta gaggtatagg tcttgttgtt tcctgaaaca gttgcaaaat acatgcctgt
    12061 tggcagccct ggcaaagaca ggtagtaggg gatgttcctg ttcaatgcag ccctggtatt
    12121 ccagatcgcc tgtcctttcc tgtcatacag ggtgatggta gcattgggta tatgatccgg
    12181 tatgctcaca gcgatatgat caatggccgg gttgggaaag atctgcaagg aacctgaagt
    12241 gattccgctt atgctgagag gtggttcttc ttcatacagc ttccattcaa aaatgccacg
    12301 gccataggtg ccgaaacgta ccgttttgat ctcttctaca tattcaacag cccaataggt
    12361 ttggtaaggt gcatccaggc cactcaggtc ataccaggta tcggtttcgg gaatgtacac
    12421 atagggagcc acttctgtgg cagcaaatag catactgtcg ccgggaagaa tggccatgtc
    12481 atataccagt gtagatggca aaccaaaact catggaaacg aagttggctc catgatcttc
    12541 actgacccaa accggtggat tgctgtaacc gcttcctcct atatacactt taccgagttc
    12601 agtggtactt ggcacgatgg atgaaccata gaaatagtga ctgtcaggtc cgtcgaaacc
    12661 gatggtcttg gtccatgtga taccccggtc atcgctgtgg aagaatttac cgtcgctcat
    12721 catcacatac cagtggtcat gatcgatggg agagtatgct atagcggcaa tagcagttcc
    12781 gcccaatcca acatcaaagt cataatcata ttgttcatag gtaatgccgg aaaagttctt
    12841 tttcaactta tacagatgat cgccaccacc ccaccagcat acttccggat catcagggtc
    12901 ttccatgatg ggagggagcc agaggtagcc ggagcccaca aaatcaccga agaacatatt
    12961 agcaggtccg gatgcatcat tgatcatcat ggcgaaaccg ggatagacgg tccacaggtg
    13021 atcgccaccg tcgctgctaa ccagatggcc atagtcgccg ctgatgagct gatcaaaata
    13081 atactggtca tcaacgctgc tgaagctgcg ctgaaagccc tgatcctgtg caccggcaaa
    13141 gatcacatca gtaacaagac gatacgtgta gaggtcatag tattgggcat tgcgcatccc
    13201 ttccattgtg atgttggtgt ttgtcagtcc gtaattatct gaagcataca gaccaccatc
    13261 ggtgctgatc agtaatgttt cttcattact gatggtatcc aggaacggca tgatgaaggg
    13321 gatatctgca tgcagtttgc tggcttcatt tccataatat tcccaccatt cattgaccag
    13381 ggtccaggtg tctcctccgt tgaaactccg gaaagtattg acgccaccga accacagata
    13441 gccggtttcc agctgactgc tgtgaaagct tccgcggctg aatgcacctg attccaccag
    13501 gcctgattca gtccagctga gacccgcatc tgatgatcgg tagatctgag tgccatcgcc
    13561 gtattgagca gtggccaggt acaggctggt accatctgta ttctgcacac ctgacagcat
    13621 atgtgtgcca ttggtggtta ctggtatttc tgccaggtag gtggtgtcac catcggcacc
    13681 atcaaccgta tagagtaagt tgcccgcatt cacatacaac ctgtcatcgc cataccttgg
    13741 tgcccacatg gaaaagttct cggtgttacc atacacatct tcccgccagt tggccacgaa
    13801 ctcaaaggtg cttcccagat catgtgaacg ataaagagcg gtgaccgcac cccagtcaac
    13861 ataatcccat tcctgtacca gtatgtaaag agtgcgttct tcattattga gcatatctgc
    13921 ttccagaatg gagccccatg ctgtcatgtt ttccagtccg gcgcaggcag tccagctcag
    13981 gccttcatcg ggtgtacggt acacggattg cacggcccac tcattggtaa ccagcaccag
    14041 ttcatcgccc accttctgta aaaaaatggc agactgtacg ttgaaattgt cattgattac
    14101 cgtccagtta tcgccttcca gatcgccttt ccaaagatta ccaccctggc tgaaagcata
    14161 gacggttccg gtttgaaaat ctatctctgt agctctaacc cggccagcag tgttgaagct
    14221 gcctacttca ttccatttgc ctacggcata tcctgcggcc agggtatccc atacatcagc
    14281 acgtgtgcct ccaagggcat actgctctga acggctcgcc gcaagttgca gacgaagttc
    14341 ccggtccatc tgccgccagt tggcacccgg agctgtgcgg tgcatctgtt ctatccaggc
    14401 ttcgcgtgcg tcgttgtgtt cggcaccttc ctcctgtttg agcggacggt ctgtgggtgc
    14461 aggcatgtcg ggttgcgggt ggacgaagta catgaaataa accatcaccg ccagggtcag
    14521 ggccagggtg acgagtacaa tggcaaaagc tatttttttc tgcatgataa tatgatacga
    14581 taaaggtaca aatacaggcc ttttcagtga tgaggtcagg catgcagcat tttcccgatc
    14641 cagtaacctg ccatacaggc aaggatggca gagaggttgt tgagtaaaat attccacaat
    14701 gccagtgtca tctggccatc ttcccacaga cgaacagttt ccagtgaaaa ggaagaaaac
    14761 gtggtgaagc caccaagcaa accggtaagt agcagtagtc tggtggtgtc attaccaata
    14821 gtgctccatc caatgccaat gagcaatgat ccggctacat tgaccaggag tgtgcccagg
    14881 gggaaggggc cggcatacca ggtcaggctc cagaggttta ccagatagcg cagtactgca
    14941 ccggcagcac cgcccaatcc tatcagcacc aaagaccaca tactccaaaa ctatacattc
    15001 agaactactt catttcttcg atgacctgcc cgatacaacc actgggttgc ggaatgcgca
    15061 gcatggccgc aatggtagga gcgatatcag tgatggcata tctggtccag gattttccat
    15121 tccttacctt ccagccatac cagagtagtg gtacctgtgt gtcataagca tactgactac
    15181 cgtgtgagct gcctgtattg ctgtaaccga accagttagg ctcgaatacg aacaggatat
    15241 ctccgcagcg tttcggatag ataccgttga tatacatttc tttatactgc gaaggtatat
    15301 tgctgtatcc aagcgcatct acttcgagca catgagccac tccatttgct gaggtgaatg
    15361 cccagttgcg cagcacatct gccacatgac cggtataggt ctggttttgc tgtacagcat
    15421 ccgggttcag atacacctgc tggttctcaa atgacaggat ccagtcagca gtaccgatgg
    15481 cagcatccag tacagagtcc atttgtgtta tcatactgag ctcactgaat acacctgccg
    15541 gtatctccag gctttgcata taacccggtg tgggtgccac accatgatct gcggtcagga
    15601 agaagagata gttgccgctg cctacttctg tatccaggaa ttggaggaat gcagcgaggt
    15661 cacgatcaag tctgatatag gtatcttctg tttccatgga gtatggacca taagcatggc
    15721 ctacatagtc tgtactggaa aaggaaacgc aaaggaaatc tgtttgctgg tctttgccca
    15781 ggccttctcc cagcaaagca gctttggcaa aatctactgt cagactgttt ccatagggag
    15841 tgcccttgat cagttccatg ctgctggcgt tgctggtcat gtgcggaaat accggagctg
    15901 tttctccttc ccggagggtt tcccagggta cattatctgc agtgctgttg gtgtagctgt
    15961 tctttggcag gaaaagatcc cagtcagtag ccatatactg gttggcccat tgctgtttgt
    16021 taaattgttc cacccaggcg ggtaatgttt ccatatacca ggtactggac acaaaggcgt
    16081 tggtgtatcc gtcaaaccaa taggctgcat cagcggcatg tccggctggc agtatggctc
    16141 cacgatcttt catggccacg ccaaccactt ttgctcttcc gttggaagca accttcagct
    16201 gatctgtgat ggtggtggtc aataaaggag cgggcgacat ttgtccggct gctgtgatgg
    16261 tacccacgcc actcaccagt gtgtctttgg tgcaatacca ttcattgtta tgtaccggat
    16321 cgaaccagtt attacccaca atgccatgca ctgctggaac agaacctgta taaatgcttg
    16381 catgtcccgg accggtatag gtaggtacat agttatagtg ggtgttctca caggagtacc
    16441 cattcttcac gagcctcctg aatccacctt ctgaataatt gttccagaat cgatagagca
    16501 gatcgtagct catctggtca acaaccacac ccacgatcag tttgggattt ggattcttac
    16561 tttccgtttg cgcatggaca gacaacagca tgctccaggc caatacaaat aatatgaaat
    16621 ttttcatgat attaaaatgc tttcagtgct gcctgaaagt tacgaaccct atgtaaaaga
    16681 aatgtaaaca ggccgggata gcttgtttga cgaggttact tttgtgatct acaatgaagg
    16741 ttatgcgtat atcactcttt ctatgtactg ttctgatttt tattgccggt atggcccagc
    16801 agaccacagt gagcgggcgg gtcatcaatg agaacaacag acaagggctt gcttttgtaa
    16861 atgtcagcct ggacgataca cacggtactt ttaccaatga gaacgggcag ttccggatag
    16921 aagatgtatc tcccggaact tatgtattgc gcatcagctg cattggtttt gacccgtatc
    16981 agtctgcatc atttgaagtg caacccgggc agccgcttga tctggatgag atttctctaa
    17041 cagagaagac catacaaata gaagagatca ccattaccga acagtataag gtatgggata
    17101 caagatatac tggaaccaat aatgtgatat ctgccaagaa gctggaacgg tttcaaccta
    17161 tcggcactga agaaatgata cgcacggttc cgggtgtgaa cattgcgggc gacatgggta
    17221 tttccaacag gccgaatatc agtattcgtg gcagtgaccc tcggcggtct aacaagatac
    17281 tattgctgga agacggatct ccaatttctc ctgcaccata tcttgcaccc ggagcctatt
    17341 ataatccacc tgcagacaga ctggatggta ttcagatcat caaaggcccg gatgccctga
    17401 tgtatggcgc aaacagcatt ttcggggtag tcaattatat aacaagaaga ccggtcaatg
    17461 atccaggcct gcgtttgcgg gtgcagggcg gtcaacgtgg ttacatggat gcctccgctt
    17521 cttatggcgg cacctgggga aacagcggcg ccgagcttat ggcactctat aagagattca
    17581 acggttatac tgacaatgca gacctgcata tgatgaatgt ttcaggcaag tggttcagtg
    17641 aattatctga cagacaaagc ctgtacacca agatcgtgat acagaaagag tatgtgaata
    17701 attcattgag cggaatgaca ccgttcacat ttgacaccga ccctacccgt catccttttg
    17761 aagctgatga atttaccagt caccggtatg gactggatgt agttcacaat ttcaaggtca
    17821 atgaaaagct cagtctgcag agcaaggtat atggatcaga tttttaccgg gattggtgga
    17881 agcagaattc tgttgtggtg catgctgctg atgtacagga gtatgtgggc gaagagaatt
    17941 atcagcaatt ctattcttat ctggatggat tggaatttac tgacgatgac tatgtacgtg
    18001 tgggtaagcg ggtaaatggc atagaaagta attctgacag ccgctggcag tacctggtct
    18061 atggagcaca tgagaaggcc aacctgaaat gggctgcagg aaacaatctg gaggctatgg
    18121 tcaaagtgca tggtgagact taccacgaca tcaccattct gggagacagc agtaagtggg
    18181 cacgaagcgg gcgcacatct gcagacgcat tctataccgt actggctacc tctggttatg
    18241 tgcgcaacga ttttcgtttt ggaagatttt ccgtcattcc catcctcagg tacgaacaga
    18301 tccatctgac caagaatgac ctgctgcaga atggtagtga tccggccaat acaggccatg
    18361 attttgggga aattaccaat gacttcggcg agtttactcc cggactgagc ctggtgttcc
    18421 gcgatatcaa ttccggctct aataactggg aattcttcac cggtgtgtac aaggcatttt
    18481 catcgcctac aacagctata tcgtttaatg agttgttgaa tggtgaggtg gtgccatcgc
    18541 ccgatattgc caatcttcaa cctgaagaaa gctggaacca ggaattgggt gcccgctgga
    18601 tcaacaacag gaattccatg aacggacagt tgtcgttatt caacatgttc atttccaact
    18661 tctattcacc tgccagggca caggcatttc agacccttgg tgcggtgcgg atcagtggtg
    18721 cagaactggc tgcttcctac aatattttga aaagtcttgg agtacatgac caggcgctta
    18781 cattgtcagg tgcatttact tatatgcagt ctgccattac tgccggcact ttatcagatg
    18841 cagatctgct gaccaaggtg gttcattctt cggccaccag gcaggaattg atcgataaga
    18901 taaatgcaga cagaagtgcc tttgaggtat atctggacgg cgaactatat gaaggtgcaa
    18961 tggacatctc acagtttggt cagatcactt ctgttgctat cacgtatggt gatggtggtg
    19021 ttacagatta tgcagtgcct tatgtaactc cggtcatctt caatgttggt ttgaactatg
    19081 agagcaaaca ttggatgatc aacagtgtgg tcaatcatgt aggacctcag ttcacagagt
    19141 tcttcaattt tgagaatgaa agtgcagacg gctccatagg tgagttgccg gcttatacaa
    19201 ctcttgatgc caatctggct tatgaactgg gagaccgggg gcaggcaaag gatatcagat
    19261 tctttgtatc aggaaagaac ctgaccaacc agatctacag aagttccaga ttgaaccggg
    19321 ccaccggagg attgtttccc gccggatttc ttcagataaa tgcaggggta agccttagtc
    19381 tgtaagaggt gtcattcgct tttcagcacc agtagagcaa cctttgattc gaaagccagc
    19441 tctctggaaa tactgcggtc aaacaagcgt tcgtagaaca atcgtttgtg tgtgaaaaca
    19501 accagcaggt ctgtaggatg ttcattggtg taggcttcaa taccattgac cacattatca
    19561 tttttgacag atacatagcg cacgtctttc agctcgccat atttctttac aagaccttcg
    19621 ttcagagcct gaatatttgt ttgttctaca gcatcatctg aagcaaaaac atgcaacatg
    19681 ctcacttctg caccaaacag tcgggcaaat tgcatgaccg caccaaattc tatttcatcc
    19741 tgatacaggt cagtggcatc tacaatatgt tctatcgggt atatttgaca gtcagccggt
    19801 acgatcatga ccggaaacct gctgtgcatg gccacattgg cagccacact tccaagaaaa
    19861 gctttctcca caccagacaa acctttcatg cccattacaa tcagatctgc cccttctgca
    19921 gcagcatatc gctgtatgat acgtgaagca ggatggccaa cttctgcatg aaaacgcatg
    19981 ggaatttctt ctccaaactc tgcagagatc tctgcagata aggtctggta gtcaaaataa
    20041 gctttggtct tcagagattc tatgatagag ctcatcatgg ctgagggcgg tgggccatcc
    20101 agatgtacca cgtttaagac caatatctcc cgatccatat tccgggttgc attcacaatg
    20161 aagcgtaaag catgtttggc agcttctgag aagtcggtcg cagcaatgat cttcagcata
    20221 aaaggggtga tttttcaggt gtgaaggtaa gcaagatggc ttcaaagcac ataaaaaacc
    20281 cgccgataaa ttcttaccgg cgggttgttc attgccattg gtacccgtta ccggatatcg
    20341 atctgttcca aatgggtgcc tgatgcagtg tccagacgaa gcatataggt gcccgctgcc
    20401 cactggctgc agtcaatgat ctgctgaccc agaagttcct ggtaagtggc tactatacgg
    20461 ccgctcagat caatgactga aaggctgtac acatcacttc cgcccggcga taccgtgaaa
    20521 gaaccactgg ccggatttgg gtaaacagac aaccagccgg aggcagcatc gttcagatct
    20581 gtagctgcac ttactgtttt gcaagcggta gaagcacagg aattttcgtt ggtcacggtc
    20641 agacaaacct caaagcttcc ttgcgctgca taagtatgta cagggttttg ttcagtactg
    20701 gtagtgccgt caccaaagtc ccagaaccat tctacagcag gatcagaatt gtcagtaaaa
    20761 ctgatcgttg cgtcagttgt gctggtgctg aaatctgcat ctatcctgtc ggccaggatc
    20821 acttccacct cgtcatatac ataacctgat tgtggattaa acaggtggag gatgatggtc
    20881 tcatcgcctt cattcagttc atcgctgatg ggaacgatgg gcaggatggc ggttgacata
    20941 cctgcgggga tggtgatctg ttcagcgatc acttcatagt caacacccat ttcggccgta
    21001 ccgtcaatcg tgtaatcgat caccaggtcc aggtcgctgg gcacaaagtt ggtgaattca
    21061 atatagccgt tggagcattc ttctgctgca accaaagctt caccatcacc ataatatccg
    21121 gtacccatac gaaggcttcc aaggcttccg gtgcgcatga atacaccggt atcgaagatc
    21181 atatcgccgg catcagccac ggcgatcttc atgtgatagg tctcgcctgc ggtcacggcg
    21241 atctcgccgg taagaaccac tgtcagaccg tcatattgta tataagagga atcgctggca
    21301 taaggatcag aagagccgtc gccgttcact acatagtatt caggatagct ggtagaatta
    21361 acgttgttga tggctaccgg cagatcggta cccggaatgg tggcaatatt caccggatct
    21421 ggaatgccgg gaccactcac ccagaaggcg aacacatcat tgaatgaagt gcccacaaat
    21481 tccagatact cttctgaccc gaatacataa tctatgcgaa ccgtatcagc agccacggtc
    21541 atatcaaact cgatatagca ggcatcatag gtcagatatc caggaaggag tgcgtccaga
    21601 tcgtcatcgc catcataacc attggctaca gaagcagaac ccaggttatt cgggcctacc
    21661 gcattgatca gtgaaccgga agtaagagcg attccactgt caatgccaac attggcatct
    21721 aaagagttgt agaatccgct ggcaccctct gcacagttcc gttcaatatt ggaaactgtt
    21781 acaccgggac cggcaagtga gaaaatgaat gtggtgtcat tgggttcttc ccatacgacc
    21841 aactgggcct gtgcgggaat ccagattcca aggatggaaa gggagaggag aagtcttttc
    21901 atcagtaaaa ttttcaggaa gttacgaaat aatactcttt ctgtgcggca ttacctttaa
    21961 cgggtcatta tgcaggaaaa agcacaggta ttccgcatga agaaattctg tattgcccaa
    22021 cagcatgcag ctatgaaagt gggtacggat ggtgtgttgc tgggtgcctg ggctgagggt
    22081 gaaggtgttc gcagcatact ggacatcgga accggtagcg gcctcattgc ccttatgctg
    22141 gcacagagat ttcctgtggc tgaggttcat gccattgaac tggatgtcca tgctgcagag
    22201 caggcgttgt ataatgcagc tgccagcccg ttcagtagca ggatacaggt gttccaggca
    22261 tcgtttcagg aatggatcag tgcggtcgtg catacctatg atctggtggt atgtaatccg
    22321 ccattcttta gcggaggctg ggaagtgcct gatgtaaaaa gacgtatggc acgtgatgca
    22381 acatttctgc ctgtagaggt gctcttttca ggagctgaga aattactttc tgcaggcggg
    22441 gcttttactt tgatactgcc tgcagaggtg catcaggtat atgccagaca tgccatacat
    22501 aacggctggt atctgtgccg gcagacaaat gtgtacagca gagcaggaaa accggtgaac
    22561 cggatcttat cagcctggta caggcaaccg cagccgatcc gaacggatga gatcattctg
    22621 gaaggccctg atggcaccag gcattcatca tatcaacgat tgacagaggc attctacctt
    22681 taacaggaac agacaataaa aaacccggtg tgtcgcaccg ggtcagtcgt atcatgtatg
    22741 atgatcagag atcatcgtca aaatcatagt cgtcttcttc gccatattct tcaccatact
    22801 cgtctatcat ctcttcgtcg tcaaagtctt cgaagtcctt gcctttctta ccaccttttt
    22861 ttccgaaatc cttttccatg ttcttgacca gatcagctat ttcatcatca tcatctgaat
    22921 caaaatccag gtcgtcatcg ccctcttcga aatcatcgtc atccaggtct tcatcatcat
    22981 cgtcatcatg ctgagcatag gggagttgat ccattccttt gaacttcttg tcctctacat
    23041 gtttgttgag gaaacggttc agtttatcga ccgatgtatt actgatgatc tcaccgaatt
    23101 cgttgatctg catgtcaaat ccttcgagtt cttcatgtac ttcaggcttg ccttccatag
    23161 gtttcttttt gggcatggta taaagttaaa tcaaggtgtt gaacaattga aaaccttttt
    23221 tcagcctgtt gtcacaactt tcttttccaa gcagggccat catggctgat accggtggtc
    23281 cctgtaatcc gcctgccagt gccagtctca gcacaggcat cacttctcct gctttcagtc
    23341 ctttgtcttc cataaaatcg tggactgttc tttccagttc tgattcgctg aattgttcca
    23401 gcaaaaatag acgctcacgg agattttcaa acaatccggc attttctttt ttccattttt
    23461 ttgcgacggt tttttcgtcg tattccagat catccccgaa tagaaatttt ccctgctcat
    23521 agagatcagg aatgaacact gcccggtctt tcatcagctc acatgccgca gccacaaatg
    23581 ccggctcagc tgaaacccct gcagcttcca gggtgggagc caacagttca gccagttcag
    23641 ctccggtttt catcttgata tgctgctggt tgatccattt ggccttctcg gggtcaaagc
    23701 gggctcctga ttttccaacc cggtccaggc tgaaagactc taccagctct tcaggcgtca
    23761 ttacttcctt gtctcccccc ggatgccatc caagtaaggc caggaaattc atataggcct
    23821 caggcagaaa accgcgttct gccagtccgc tgatcttttc tcccgttaac gggttttccc
    23881 agttcagggt ataaatggga aaacctaacc tgtcgccatc ccgcttgctc agtttgccat
    23941 tcccttccgg tttgagtacc agtggcaggt gtgcgaaccg gggcatggtg tcttcccaac
    24001 ccaggcttct gtacagcatg atgtgtgtag gggcactggg cagccattct tcaccacgaa
    24061 ccacatgggt gatgtgcatg aggtgatcat ccaccacatt ggccagatgg taggtaggca
    24121 tgccgtcact cttgaagagc actttgtcgt ccagctgact ggtattgaat accacccatc
    24181 cgcgaatgag gtcttcgaac ttcagttctt cattcctggg catcttcagg cggatcacat
    24241 aagcatcgcc ggaggccagc cttttttctg tttcatcctg actcagtgac aggctgttct
    24301 tcatgtactg acgggtgata tgatcatatg ccggactcgg gttgccctgg ctgctcatgc
    24361 gctcccgcat ctcttccagc tctgccggtg tgtcaaaggc ataataagca tgaccatctt
    24421 tcaccagttg ttccgcatac tgccggtaca tgtctttgcg ttcgctctga cggtaaggac
    24481 cataaggccc gcccacatgg atgccttcat caatggtgat acccgcccat tggagtgaat
    24541 ccacaatgta ttcttctgca cccggaacaa aacgggtctg gtctgtatcc tcactgcgga
    24601 acacaaaggt gcctccgtgt tttttgacaa agaggtagtt gaacaaggct gttctgatac
    24661 ccccgatatg ctgtggccct gtagggctcg gagcgtagcg cagcctgact tttccggcat
    24721 tttccataag cgggtgtaaa gttaaaaatg attatcattg ttgcccttct gctgcatgta
    24781 ttttacccgc acaccggctt atctgaaatc tttgttcggc ggtctcacct gggatgtgcc
    24841 tgaaacagat gccatctttc tgaccttcga tgacggaccg gtgccgggag ttacagacaa
    24901 ggtgcttgac atgctggccg catcagaagt gaaagccacc ttcttttgca tcggccataa
    24961 tgtgcagaaa cacccggaat tataccggcg aatcctggac gaaggccatg cggtgggcaa
    25021 ccataccttt caccacctga acggctggcg aagcaacgat gaaacctatc tggaggatgt
    25081 agagaaatgt gctgatctgg tgtccagcag gttattcaga cctccttacg gcaggatcgg
    25141 ttaccggcag atgcagctgc tgaagcaaca atacaggatc atcatgtggg atgtactgag
    25201 cggcgatttt gatgaactac taacggccga tgactgtgcc aacaatgtat tgtcaggtat
    25261 gcgaggcgga agcatcattg tctggcacga cagtgagaaa gctgcaccca gaatgttgcc
    25321 ggctcttgag cgggtattgc ataccatccg gatcagaact ttgagaacgg aaacactacg
    25381 tggatgaaac tgatagacag ccatgcgcat atctatgttg cagactgggg cgaagatcag
    25441 caggccatga tcctccgggc gatggatgcc ggggtggaag ctgtgatcat gccccatata
    25501 gatgaagaaa cttcagaggt gttgctgcag ttataccaga actatccgga ctggtgctgg
    25561 cctgccatgg ggttgcatcc ctgcagtgtg aaagatgatt tttcggaact cctggatgac
    25621 tatgagaaac ggctcgccaa caattccatt ccctggaagg gtataggtga gacgggcctg
    25681 gattattact gggatctgac ctataaaaag gagcagcaaa aagcttttga ccgtcagctt
    25741 tcgtgggcct gcgaactgga tcttcccatc atcattcaca gccgagactc gctggacgat
    25801 tgtatcagca tggcgaagca acggcaggac ggcaggctga aaggggtgtt tcactgtttt
    25861 tccggaaacc gtgaacaggt gcagcagata aatgatctgg gtatgtatat aggtatcggg
    25921 ggtgtggcca cgtttaagaa cggcggactg gacaaggtgc tgttaccgga agaccttgat
    25981 ctgatggtac tggaaactga ctctccttac ctggcacctg cacctcaccg cggaaagcga
    26041 aacgaacctg catatctgcc attggttgtg aagagactgt cagagatact ggatctgacc
    26101 gaagaagaga tagcccgaac aaccaccgca aacagcagca aactattctc tcttcctgct
    26161 tgattttacc tgcaaatgcg tagttttgtc ctcccttatc aggcatggag aggtgtccga
    26221 gtggttgaag gagcacgcct ggaaagtgtg tataccccta aagggtatcg agggttcgaa
    26281 tccctccctc tccgcaaccc aaaaacccgc cctacgcgaa agcgtggggt ttgttttttc
    26341 cggcagaccg accaggctcg cctgaggcgg aatgacggga aaaacaaacc ccggaccctg
    26401 aaaggggacg gcgggtgaac aggttaagcc cccccacttc aagggatcgc gaagcaatcc
    26461 cgtttgagag ttggagtttg agagtggagt gcaagaagaa acaaaccccg gaccctgaaa
    26521 ggggacggcg ggtgaacagg ttaagccccc cca
//
LOCUS       DAOMAX010000008        23589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_222_length_23589_cov_4.450667, whole genome shotgun sequence.
ACCESSION   DAOMAX010000008 DAOMAX010000000
VERSION     DAOMAX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_23589_cov_4.450667"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK742_01160"
     CDS             <1..284
                     /locus_tag="PK742_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQU75224.1"
                     /translation="REAILATALETIQLHGLAIYGADAHASAIPATTDLSIPCAIVMG
                     SEDRGISSEARAMLTETVSIPMARDFDSYNVSVAAGMLLYEAMKQRSGS"
     gene            complement(279..737)
                     /locus_tag="PK742_01165"
     CDS             complement(279..737)
                     /locus_tag="PK742_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75225.1"
                     /translation="MKVYKDLTDFISNLNQCGVRYLLVGGYAVQLHGYPRFTQDMDIW
                     VDRTSENYRKLVTAFGQFGLPLFDMTEEVFLTHPDFDVFTFGRPPVSIDIMVLVKGLN
                     FSDAYADKKIMEFDEVQVDVLSLEKLLVAKKASGRFKDLDDIEHLKAGLS"
     gene            complement(734..943)
                     /locus_tag="PK742_01170"
     CDS             complement(734..943)
                     /locus_tag="PK742_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75226.1"
                     /translation="MQFRLDKTAFKMRAVQEADDQYKIYASMTPHERWETGWFLVCVA
                     YRWPEDQIMYLDRTAFRMGKQSKQE"
     gene            complement(956..1936)
                     /gene="pfkA"
                     /locus_tag="PK742_01175"
     CDS             complement(956..1936)
                     /gene="pfkA"
                     /locus_tag="PK742_01175"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQU75227.1"
                     /translation="MAELKKIAVMTSGGDAPGMNACIRAVVRNAIFKGVEVVGIHRGY
                     EGMIDGDFENLESHKVSNIIQRGGTILKSARSERFRTKEGRQKAFEQMKFAGVQAFVA
                     IGGDGTFRGATVFEKEHGIQCIGVPGTIDNDLFGTDYTLGFDTAINTAIDAIDKIRDT
                     ADSHNRLFFVEVMGRDAGFIALSCAIAGGAEAVVIPETETNIDNLITILEKGWYHKKS
                     SAIVVVAEGDEAGGAYDIARLVKEKFSEYDTRVAILGHIQRGGNPSVYDRILGSRLGV
                     GAVDALLAGRRNEMIGVTNGKISFTPFSKATKHHQEINKNLLQLVEILSI"
     gene            2047..3474
                     /locus_tag="PK742_01180"
     CDS             2047..3474
                     /locus_tag="PK742_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU75228.1"
                     /translation="MTQSSTSRNGMAFVSLVSVFFFWGFVAASNDILIPVFKSALQME
                     QWQSQMISFAFYVAYTVGSLAYFGISALMKRDILNRIGYRNGLALGLVISALGTLLFI
                     PASNSASFGLLISGLFIVGLGFSLQQIAANPLAINMGDPSKGNMRLSLAGGINNVGTT
                     IGPVIVAFAIFGGLPDSAADMNLTAVQIPYLVLGAAFIIAALFIRFSSVPDHIGDKQM
                     PSGGFSQTLRYPQVWMGMIAIFVYVGVEVSTASNLPEFMRQKLHVEEHQIAPYVSLFW
                     ASLMIGRWTSAAGAFDLPESTKNRLSILLPFVAFAIFALVNTIAGHEISQFYPYLFLV
                     ILLAVADAISQGHPVRQLLLYSFLGIACLLVGIFSSGMVSVFAFIAVGLFCSTLWPCI
                     FTLGIAGLGEKTNTASSLLIMMIMGGGFVSLFQGWLAGDGLLGIQYSYFAGVVCFLYL
                     AFYAWKVGAIHKKHHVLVETAEGDH"
     gene            complement(3587..4522)
                     /locus_tag="PK742_01185"
     CDS             complement(3587..4522)
                     /locus_tag="PK742_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQU75229.1"
                     /translation="MEKILLIGACGQIGAELTMQLRAAYGDGNVVAADVKEPNSEIRE
                     SGPFEHLNVLDAASFGDILERHSPTQIYNLAAMLSATGEKYPLKAWDLNMNGLLNTLE
                     LSIIHEVKKVYWPSSIAVFGPTTPRVMTPQETVMEPNTIYGISKQAGERLCEWYHQKK
                     GVDVRSLRYPGIISYKTEPGGGTTDYAVDIYFQAKRKGSYECFLKEGTALPMMYMPDA
                     IRATMELMEAPANTISVRSSYNLAAMTFAPEEVAASIQKEIPDFAVSYAPDFRQAIAE
                     SWPASIDDSVARKDWGWKPEFDLDAMTKDMLEHVR"
     gene            complement(4567..5856)
                     /locus_tag="PK742_01190"
     CDS             complement(4567..5856)
                     /locus_tag="PK742_01190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU75230.1"
                     /translation="MKRVLIISYYWPPVGGAGVQRWLKHARYLPDFGWKPIIYTPENP
                     DLSITDESLLQDVSQDQEIWRTPIREPYDLYRKLVGKREVAVNHGLMDTTEKGGFFSR
                     LGMWVRANAFIPDARMFWIRPSARYLIGRLKKEPVDAIISTGPPHSMHRIALQVHRAT
                     GIPWIADFRDPWTNNDVYHRLPLGKRAERIHLRMEKEVLRTATGVVTVSWQWAEDMKQ
                     ISGREVEVITNGYDEADFSGAVSLPKDVFELHHVGSLYADRNPVVLWKLLGERYRSSE
                     PWKKHLRIHLTGKTDQSVLAAIREAGLQDVLQYDDYLPHSEVIEAMRQAPVLLLLLND
                     APDIMGRIPGKLFEYLAAGRPVLGIGAAAGDSARILNESKAGKMFDLDDELGMAAWLD
                     NSMQAWLDGSLEVQQAASGMYSRRSCAKQYAELLNQV"
     gene            5946..8408
                     /locus_tag="PK742_01195"
     CDS             5946..8408
                     /locus_tag="PK742_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75231.1"
                     /translation="MSGTNFRQTAIEWGIAILIFLALSIAFFTPVFQGKILIQTDALN
                     FKAMSKETLDYNASHDDAALWTGTMFGGMPTFLIHLEHSGNLFAFLNKAIGLWLPRPA
                     NYLFMSCLTFFILLRSFKVRNWLAIFGAISFAIGTYMISFIEAGHNTKVHAFAIMPLV
                     LAGVNYLFRGKTWLGFSVALLGMCLEIDANHIQITYYMYFLLFCWFAAELVFAYREKK
                     MVSYWKVFGLFALISVLSFTANFSRLYTTYVYSEESIRGGSELTKEGDLNANYAGNGL
                     SKDYVFAWSYGMDESMTLLFPNFAGQASGKSFLEDEDSKSMAYIRQLYARQPDKAQQM
                     QQFTGKYWGPLQFTSGPVYLGAVVAFLFLLGAFAAENRMRWWLIAGSVVSLMLGWGDH
                     FKAFNYFMYDHFPMYNKFRTVMMALIIFCLTAPLLGFYILEKYMREGDTAQKFKAWKY
                     ALIVIGVLTLLVLIPVFDPSGPRDEQLMSAYGQDPDIAGLVSAVHADRVSMIRMDGLR
                     TLVFIALSAGLIWLYLKGRVKAIAAVLGIGLLSVIDLWTVNSIYLNADNYDSPDYYAR
                     YFASRLPVINDEDPNYRVLNTTVRFDQDGVTPWSYHSIGGYHAAKLRRYQDVIDGYLN
                     NGAMNVINMLNAKYVIVSNNGQLATQRNPGACGNAWLVDSVVFVNGPDAEFEALKELE
                     PRRYAVVDEQFKDQLQQVPERDSTAGIIMLNYSPNEITYKFKGNSAQIAVFSEIFYRG
                     NEDWKAYIDGEYQDHFRADYLLRGMLIPAGEHDITFRFEPSSYYTGRKISLGASSILL
                     LLIAAGFWMSWRKDRENGSATE"
     gene            complement(8398..9672)
                     /locus_tag="PK742_01200"
     CDS             complement(8398..9672)
                     /locus_tag="PK742_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75232.1"
                     /translation="MLPTIRFQLETDMQAEYAWIIRAWGRYSGFTPLFVETEPELTVS
                     ELGISDIRLSHFFRNTYLHGDHHAKAYFATAPVHLHAGGQTDHLSTAFYMLSCQQEYA
                     PFEGDKYGRFAFADSWQYRFDAADKNLVAFHFDQLTKSLPLVQQKILKQDHASRLFCT
                     HDIDTVFGAWTEQRKALLKKGRVGTLLQLMFRHITGRYDYLELEKILAIEDAYDVRSV
                     FFWLTERGRGRNGIENADYHIDDPAIRKKMQLVSAAGGSNGLHKSALQTTYSEELERL
                     DTNNAISNRNHYLRFFMPDTWRVMEQAGVALDATLGFAEQPGFRAGYGLPYRPFDVSG
                     RREIALTEVPLLVMDTTYRHYLKKDPATAGKEIINLLDKHRQDAVINILWHNNYFFDL
                     AEPGWLQVYKDVLAWARDNAIRSVMPEDLIIQ"
     gene            complement(9743..11296)
                     /locus_tag="PK742_01205"
     CDS             complement(9743..11296)
                     /locus_tag="PK742_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQU75233.1"
                     /translation="METVMTHNPIAAELEALGIKETNSGVSTGTRWLTGSGKTVESYS
                     PVDGKLIATCTSASREEYEELMQQATEAFKIWRMMPAPKRGEIVRQVGDELRKYKEPL
                     GKLVSYEMGKSLQEGMGEVQEMIDICDFAVGLSRQLYGLSMHSERPLHRMYEQWHPLG
                     VVGIISAFNFPVAVWSWNAAIAWVCGDVCIWKPSSKVPLSAIACQQIISRVFERNNIP
                     EGVSCLMVSHSAIGEEMAKDKRVPLISITGSTRVGKRIGGIVGERLGRVIQELGGNNA
                     IIVSEHADIDMVVRGAVFGAVGTAGQRCTSTRRLIVHESVYDAVKDKMTRAYAQLKIG
                     DPLDQHNHVGPLIDTGAVKDYLAAIEKVKEEGGRFAVEGGVLEGEGYESGCYVKPCIA
                     EVENHYQIVQDETFAPILYLIRYKTIEEAIELQNGVPQGLSSAIMTGNLQEAERFLSH
                     AGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINYSTELP
                     LAQGIKFDL"
     gene            11397..11897
                     /locus_tag="PK742_01210"
     CDS             11397..11897
                     /locus_tag="PK742_01210"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HQU75234.1"
                     /translation="MKQILFAATLLIAHCGFSQHVLELLEEQHPAAGKVEDFSWMAGS
                     WSCAIWDGVAEEYWMVPQSNSMAGMFRFSADDSLRFLEIFTLEQSGRSVTLKLKHFDA
                     GLQGWEEQDDCVSFVLVYQEPNRAYFDGMTYILRDPDHLDVFVLMDHGDGTQEELHFP
                     YFRISR"
     gene            11968..13506
                     /locus_tag="PK742_01215"
     CDS             11968..13506
                     /locus_tag="PK742_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HQU75235.1"
                     /translation="MYHTFFRHRNGTVLLLVSLLFLASCQKDRSLGEQITLQELVSEN
                     LQIELNPNGNTPLAARLSFTADVPVQVSYTIEGEEPISHTFPGFSATQRVPILGLYPD
                     TDNEVKVTISDQAGNFASATLHVETGPLPDYFPAIETNVNTSLRQEGWTLTEIGIGLG
                     EAFMSVPSIIDASGNVRWYIVLDAYTDLCFPIKQKSNGNLLISFYEHIYEYTMLGGVV
                     NDIYLPGWYQHHETREMPNGNLLVSVDKIGESTVEDHILEVTPSGSIVQVWDMRPILD
                     MDRYDLVEDEVDWFHNNAVYYSEADDCLLVSGRNQGVVKVNRNNEIVWILSPHQGWGT
                     ATNGTNTQDFLLTAIDANGDPYPEEVQQGTAPADDFDWCWGQHACMLLPNGNIFLFDN
                     GFNRQFIAGVPSYSRGVEYRINEENMTVQEIWQYGKERGPECWAPIISDVDVLDNTNR
                     LITSGIVFGAEPYAKIMEVSYPDRTVVYEITLHFKNLLAAGGLAWGGLDLNYRAERIH
                     IFPQ"
     gene            complement(13547..16561)
                     /locus_tag="PK742_01220"
     CDS             complement(13547..16561)
                     /locus_tag="PK742_01220"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQU75236.1"
                     /translation="MTRLFLFSALILKVVITTEAQKPSYWQQHVDYDISVALNDSMHM
                     LNGNISMIYTNNSPESLTEIWMHIWPNAYENAHTALGQQFIDDRNREMLLASAEELGF
                     IDELNFQVGGKSVTWEYHPEHRDIARLMLPEPLQSGASVTIATPFRVKIPSSDFSRLG
                     HSGNSYQITQWYPKPAVFDQSGWHAIPYLNQGEFYSEFGNYHVTITVPSNYIVAASGN
                     LLTESERNFLLQKAAETAAMENFSSDNSFPPSAATTKTLEYELKDAHDFAWFADKRFH
                     VLHGQAQLPYSGRTVDSWSYFTNFQTGLWMKSIDYLNRSVIAYSNYAGEYQWDVVQAV
                     EGALSAGAGMEYPTITIIGGSANAAGLDNVITHEVGHNWFYGMLGSNERDHAWMDEGI
                     NSYYENRYMTTYYPNANPLEPFLGNAARFINADALEGKNTLDMAWYLILGLERMNKSQ
                     AIDQHSNNFTQLNYGLLVYMKTAYLMRYMADFLGQGQFDRVMGKYFEEWNNKHPQPED
                     IRRVFEEETGENMAWFFEGLLKDDRSLDYKITEILDGQTTMAVRVHNNTDIAGPFPVS
                     VMRGDSILRTEWHRGFTGTQTIYVKKLSSSEEITKVRIDAHNTIPDNNRENNTMRVSG
                     LFKKVEPLALKPFFSPDNPDRTTISYLPLVGFNVNDGWMVGLGLWNSTFPAPKLEWVA
                     APLYSFRAQGISGQGSLGWNIYPERGAFSRVRLSVSGASYHVDSLAESYYTRLQPAID
                     IDIRKKQLKDPVKQSIRYRNVRIGETYVLGILENGEQYKLDTAFWFNELSYTVKNRHL
                     QHPYQATLTAQQGPDLIKLFADVTTGIHIRKYEIEARLFAGWMSYFKEDPYSRYAYSL
                     TGVSSYNDYLYDDILPGRNATDGMWSNQITRRDGFLKLPADTIALGLQDDVLLALNLE
                     FPVPKLPLAFFADAAYVPGDLPASISGFQYDAGVMIRLLNNVIEVYFPLLASDGLVDP
                     FNADYGTYMSHVSFLVNFNGINPFELMRKLEF"
     gene            16686..18095
                     /locus_tag="PK742_01225"
     CDS             16686..18095
                     /locus_tag="PK742_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU75237.1"
                     /translation="MLRPEQDDHAEEAGLQELIEQFEAFLEKKEYSFFDDDALERLYE
                     YYENRLDEGHMEAVADLAIAQNPYSGDFLVRKAELLFNRKDYQASLELLDKAVVFDAT
                     NLDIYLLRSEIMVESNRIEEALELLQDALRLADRDEKDVILTEISDVFEIQEDFHAAF
                     DCLRDAIKHNPSNEAALMKIAHLAEMTDRYDDSLDIHLAITEEHPYNWMAWYNAGRAY
                     AGLGLYEKALDAFEFTMAIQEDFDLVYRDVADIYFRLEDLVKAISTFETAQEKSGGFE
                     DYSFRIGMCYERKNDLKKARFHYRKSTRQDPYLDEAFFRIAETYRQEDRFEPALVNYK
                     KALRIDETNELYMTCIIAVYRMLGREEEVLQYLQRLTQARPDILNYWLDLLIFRWEHE
                     QFEAGLENVQEATERCGHFVEFDYLRSLLLWYAGQQQESLRVLAQALDTDKARHTILL
                     ETDPSFLLKEAVMRVMNAQ"
     gene            complement(18089..18679)
                     /locus_tag="PK742_01230"
     CDS             complement(18089..18679)
                     /locus_tag="PK742_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQU75238.1"
                     /translation="MLNKTFWEHRYANDQTGWDLGAVSPPIKAYVDHLQDKYAPVLIP
                     GCGHGYEAEYMLEQGFSDVTVVDIAALPLERLRSRVGDRSGLKTIQSNFFDHQGQYQL
                     ILEQTFFCALDPALRQDYMQHMHSLLKPGGHLAGVLFNVEFEKEGPPFGGEESDYRKL
                     MKPWFHILLMEPCENSVQPRMNTELFFVAEARNDAH"
     gene            18741..19499
                     /locus_tag="PK742_01235"
     CDS             18741..19499
                     /locus_tag="PK742_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HQU75239.1"
                     /translation="MNFELNQLPERSSKPRTNGLTMVMDKGLSIREAEDMLSVSGPYV
                     DIVKLGFGTSYVTPNLEEKIKLYQHAGLGVYLGGTLLEAFIIRNRYNDFLQLLDRYGL
                     QHVEVSDGSIQMDHDEKCRLIRELSSRATVLSEVGSKDAKKELAPYLWIELMQKEIDA
                     GSWKVIAEARESGTVGIYRDKGEVREGLIDEILTKIPQDKIMWEAPMKSQQVWFIKLL
                     GANVNLGNIAPNEVIALETLRIGLRGDTFHHFLK"
     gene            19499..20434
                     /locus_tag="PK742_01240"
     CDS             19499..20434
                     /locus_tag="PK742_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HQU75240.1"
                     /translation="MSVFRKYFPVTLKGMAMGAADVVPGVSGGTIAFISGIYAELLNS
                     LRSVNLKALRILFREGIPAFWKHINGWFLLFLLTGIGISIVSLSRMILYLLENYPPLI
                     WSFFFGLVVASAIYVAGTIKERNWKVVLSGLAGIVIAGWITIASPAQGSDSLPFVFVT
                     GAIAICAMILPGISGSFILLLMGMYEFILGALHDLKISVIITFMAGAGIGLVSFSHLL
                     SWLLKKYHDLTVALLAGFMIGSLNKVWPWKEVLESRFIDGEEKVIQEANRLPATYSEV
                     TGEPNHLLFCILLFLTGFVLIYSLEKFAGKKHQLI"
     gene            20440..21252
                     /locus_tag="PK742_01245"
     CDS             20440..21252
                     /locus_tag="PK742_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQU75241.1"
                     /translation="MDSASDPYPNDDHASLQDEHLKLYGLIGKSLTHSFSQKYFLSKF
                     KNEELEDHYYALFPLDDIDDIRLLLEVHKNLKGLNVTIPYKESVIPYLDELDPVAAEI
                     GAVNCIYMDDFEKKGYNTDWLGFRDSIFPLLRAADKNALVLGAGGSSKAVCYALKQLG
                     LSYKVVSREAGKSDLTWDMLTTEVIAQHTVIINTTPVGMWPDADAMPPVPIDAISPQH
                     ICFDLIYNPEQTRFLQEAASRGAVTSNGQQMLELQAAHAWKIWNTPIEEKEE"
     gene            complement(21255..22133)
                     /locus_tag="PK742_01250"
     CDS             complement(21255..22133)
                     /locus_tag="PK742_01250"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQU75242.1"
                     /translation="MAVILLATGVCSGQTKYRDAEVGINLGFSNFLGDLGGSRMEGRA
                     LWWDIDPEVTRPAIGVIYRQEVVPHISVRLNAYYTQIRGADSLSENDFRSYRNLSFKS
                     PLTEVCLYVDYTLRRLTGPNRERFTPFVYAGAGMCWFNPQALYEGQWVELQPLGTEGQ
                     GLPQYPDRQPYAKYTFVFPFGGGIKYLTRSNWVFGMETASRLSLSDYMDDVSNSYPNP
                     DYFFGFYDPATATMVAALSDMSDHTRPDLISLETGRGNPKNRDAYVLGGLFTITYHFD
                     QVIRPKPGSCFFKEKR"
     gene            complement(22216..23349)
                     /gene="rffA"
                     /locus_tag="PK742_01255"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(22216..23349)
                     /gene="rffA"
                     /locus_tag="PK742_01255"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQU75243.1"
                     /translation="MIPFNKPYVTGLEQDYLEQAVRSGRLSGNGIMTRSVCNRLQNIT
                     GASNVLLTTSCSTALDMAAILSGVGHGDEVIVPSFTFPSTANAFVQRGAVPVFADSTD
                     LHPNVSADTIAHLITERTSVIVVTHYAGVPCNMDAINRLAGQHNILVVEDAAMALGST
                     FNNKPVGSLSPLTAFSFHETKVLGCGEGGALALQRSDLMSRAEIIWEKGTNRAAFQRG
                     QSDHYEWVDIGSSFLPSEIQAAWLMAQLKEAESILNKRAEQWNRYYLSFQHVLPDGFA
                     LPVIPENVHHNGSIFYVQCPDRQVRNYLISALQRAGIMAVFHYLPLHSSKMGQKWRSD
                     KTCNLAEKWSDTLVRLPLYHTLSVQDQQFIIDQVVLACRHSVN"
     gene            complement(23346..>23589)
                     /locus_tag="PK742_01260"
     CDS             complement(23346..>23589)
                     /locus_tag="PK742_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75244.1"
                     /translation="SILQDGVRNFYFPAAVRIHGGHLPVLVVRHGERKHGSSTYTFSR
                     QLKLALQALSYALFHRSGNKTLPYQTETIIYTKFDE"
BASE COUNT         5952 a         5828 c         5498 g         6311 t
ORIGIN      
        1 gcagggaagc cattctggca actgcactgg agacgataca gttacacgga cttgcgatct
       61 acggagctga tgcccacgcc tctgccattc cggcaacaac agacctttcc attccctgtg
      121 ccattgtcat gggaagtgaa gacagaggta tcagcagtga agcacgtgct atgctcaccg
      181 agaccgtaag cattcctatg gcaagagact ttgatagcta taatgtatca gttgctgcag
      241 gcatgctcct ctatgaggcg atgaaacagc gatccggttc atgacagacc agccttcaag
      301 tgctcaatat catccaggtc tttaaatctg cctgaagctt ttttcgctac aagcagtttt
      361 tccaatgaca gaacatctac ctgtacttca tcgaactcca tgatcttttt atcagcatat
      421 gcatcagaaa aattcaatcc cttgaccaga accattatat cgatcgaaac aggggggcga
      481 ccaaaagtaa aaacatcaaa atcagggtgt gtcagaaaaa cctcttcagt catatcaaac
      541 aaaggcaggc caaactggcc aaaagcagta acaagtttcc tgtaattttc agaagttctg
      601 tccacccata tatccatatc ctgcgtaaat ctggggtaac catgtaactg gaccgcatat
      661 ccaccaacaa gaaggtacct gaccccgcac tgattcagat ttgaaataaa atcagtcagg
      721 tccttgtaaa ctttcattcc tgttttgatt gtttacccat acgaaatgca gttctgtcca
      781 aatacatgat ctgatcttcg ggccatcggt atgccacaca aacaagaaac catcctgttt
      841 cccatctttc atgaggggtc atcgaagcat agatcttata ctgatcatca gcttcctgaa
      901 cagccctcat cttaaatgcg gtcttatcca gacggaattg catactacta aattatcaaa
      961 tagacagtat ctccaccagt tgcagcaggt tcttgttgat ctcctgatgg tgtttggtcg
     1021 ctttggagaa cggcgtaaag gagatctttc cgttggtcac gccgatcatt tcattccttc
     1081 tgcctgccag cagggcatcc acagcaccca ctcccagtct gcttccaaga atacggtcgt
     1141 acaccgaagg attaccgcca cgctggatat gtccgagaat tgcgacgcgg gtatcatatt
     1201 ctgaaaattt ctctttgact agccgggcta tgtcataggc tccgccggct tcatcgcctt
     1261 ctgctaccac cacaatggca gaactcttct tatgatacca gcctttttca aggatggtga
     1321 tcaggttatc gatattggtt tctgtttccg gaatgaccac tgcttctgct ccaccggcaa
     1381 tggcacagct cagggcaatg aagccggcat ccctgcccat cacttctaca aaaaacaaac
     1441 ggttatgaga gtcggcagta tctctgatct tatcgatagc atctatggct gtgttgatgg
     1501 ctgtatcgaa tcccagtgta tagtcggttc cgaacaggtc gttgtcaatg gtacccggca
     1561 ctccaataca ctgtatgcca tgttcctttt caaataccgt agcaccacga aatgtgccat
     1621 caccaccaat agccacaaaa gcctgcacac cagcaaattt catttgctca aatgccttct
     1681 gcctgccttc tttggtacga aaacgctcac ttcgtgcaga tttcaaaatg gtgccgccac
     1741 gctggatgat attactgacc ttgtgtgatt ccaggttctc aaaatcgccg tctatcattc
     1801 cttcatatcc acgatggata cccaccactt ccacaccttt aaaaatggca ttcctgacaa
     1861 cggctctaat gcaggcattc attcccggtg catcaccacc ggaagtcatt actgctatct
     1921 ttttcaattc agccatattc tgtttaaaaa taagcctgca atattgcgaa attattttcc
     1981 aaatttgaac ctgtggtatc ctgttgcaga ccggcacggg ccatattgca tcaataagaa
     2041 aagataatga cccaatccag cacctcccgc aacggcatgg cattcgtgtc tctggtatcc
     2101 gtattctttt tctggggctt tgtggccgcc agcaatgata ttctgatacc ggtgttcaaa
     2161 tctgcacttc aaatggagca gtggcaatca cagatgatca gctttgcctt ttatgtggca
     2221 tatacggttg gttctctggc ctattttggc atatcagctc tgatgaagag agacatactc
     2281 aaccggatag gctatcgcaa tggtctggca ttagggctgg tgatctctgc acttggtacg
     2341 cttttgttca ttcctgcatc gaactccgct tcgtttggcc tgctgatctc cggactgttc
     2401 attgtaggtc tcgggttctc tctgcaacag atcgccgcca atccgctggc catcaatatg
     2461 ggcgatcctt ccaagggcaa tatgcgtctg agtctggcag gaggcatcaa caatgtgggt
     2521 accaccatag gaccggtcat cgtggcgttt gccatcttcg gaggcttgcc tgattctgct
     2581 gctgacatga acctcactgc agtgcagata ccttatctgg tgctgggtgc tgccttcatc
     2641 attgctgctt tgttcattcg tttttcttca gtacctgatc atataggtga taaacaaatg
     2701 ccttccggag ggttctctca aacgcttcga tatccgcagg tctggatggg tatgatcgcc
     2761 atattcgtgt atgtaggtgt agaggtttcc accgcctcca atctgccgga attcatgcgg
     2821 cagaaactgc atgtggaaga acaccagata gcaccgtatg tcagtttgtt ctgggcaagc
     2881 ctgatgatcg gtcgctggac ctctgctgcc ggtgcattcg acctgccgga gtccaccaag
     2941 aacagactga gtatcctgtt gccttttgtg gcgtttgcca ttttcgccct ggtcaacacc
     3001 atcgccgggc acgagatcag ccagttctac ccatatctct tcctggtcat cctgctggct
     3061 gtagcagatg ccatcagcca gggacatccg gttcggcagt tattgttgta ttcttttctt
     3121 ggaattgcct gtttgctggt aggtatcttc tccagcggca tggtgagtgt atttgccttt
     3181 atagcagtag gattattctg cagcaccctg tggccctgca tattcacact tggtattgcc
     3241 ggtttggggg aaaagaccaa taccgcatcg agtctgctca tcatgatgat catgggtggc
     3301 ggatttgtaa gcctttttca gggttggctt gcgggcgatg gtttgttagg tatccaatac
     3361 agttactttg caggtgtggt ttgtttcctg tatctcgcct tctatgcgtg gaaggttgga
     3421 gccattcata agaaacacca tgtgctggta gaaacagcag agggagatca ctgatcctga
     3481 agacgggtca gagtttttgg ttgttggtct ttggttatta gttattggta gttagcacat
     3541 ggttgttaga agtccgctac ccgggaccct ttcaaccttc acccttctat ctcacatgct
     3601 ccagcatgtc tttggtcata gcatccagat cgaattcagg tttccagccc cagtcttttc
     3661 tcgccacact gtcgtctatg ctggcaggcc atgattctgc tatcgcctgc ctgaaatcag
     3721 gtgcatagga tacggcgaaa tcggggatct ctttctgaat acttgcagct acctcctccg
     3781 gagcaaaggt catggcggcc aggttataac tgctgcgcac agaaatggtg tttgcaggag
     3841 cttccatcag ttccatggtg gcacggatgg catcaggcat atacatcata ggcaatgcag
     3901 tgccttcctt cagaaaacac tcataggatc cttttctttt tgcctggaag taaatatcaa
     3961 cagcataatc tgtggtacct cctcctggtt cggtcttata gctgatgata ccgggatacc
     4021 tcaggcttct tacgtccaca cctttctttt gatggtacca ttcgcacaga cgctctcctg
     4081 cctgcttgga aatgccatag atggtattgg gttccatgac cgtttcctgt ggtgtcatga
     4141 cacgaggggt agtggggccg aacacagcaa tggagcttgg ccagtaaact ttttttacct
     4201 catgaatgat ggataactcc agggtattca ggagaccgtt catgttcagg tcccaggctt
     4261 tgagcggata cttctcgccg gtggcactga gcatggctgc caggttgtag atctgtgtag
     4321 gactgtgccg ttccaggata tcgccaaatg atgcggcatc cagtacattg aggtgttcaa
     4381 aaggcccgct ttcacgtatc tctgaattcg gttcttttac gtcggcggct actacatttc
     4441 catcgccgta ggcagccctc aactgcatgg tcagttcggc accgatctgt ccgcaggcac
     4501 caatcagaag gatcttttcc atgtaataac ttgtaggcac aaatgtagca gaaaacgggt
     4561 tgttgatcag acctggttca gcagttcagc atactgtttt gcacaggacc ttcttgagta
     4621 cattcccgat gctgcctgtt gtacctctaa acttccatcc agccaggctt gcatgctgtt
     4681 atctagccac gcagccatac ctaattcatc atcaagatca aacatcttcc ctgccttcga
     4741 ttcattcagg atccgggcac tatcgcctgc tgcggcacct atacctaaga ccggtcggcc
     4801 ggcagccagg tactcaaaca atttgccggg aatacgaccc atgatatccg gagcatcatt
     4861 cagcaggagc aacaataccg gtgcctgtcg catcgcttca atgacctcgc tgtgaggcag
     4921 ataatcatca tattgcaaaa cgtcctgcaa accagcttcg cggatggccg ccaggacaga
     4981 ctgatcggtc ttaccggtaa gatgaatccg taaatgtttt ttccagggct ctgaactgcg
     5041 gtaccgttca cccagcaatt tccataatac aacgggattt ctgtcggcat acagtgaacc
     5101 aacatgatgc agttcgaata catcctttgg cagagagaca gctcctgaaa aatctgcctc
     5161 atcatatccg ttcgtgatca cctccacttc tctgccggat atttgcttca tatcctcagc
     5221 ccattgccag ctgacagtga ccaccccggt tgcggttcgt aaaacttctt tctccatgcg
     5281 tagatggatg cgttctgccc tttttcccag aggcagacga tggtacacgt cgttattggt
     5341 ccatggatca cggaagtctg cgatccacgg aatgccggta gcccggtgca cctgcagtgc
     5401 tatgcgatgc atgctgtgcg gcggacccgt ggagatgatg gcatcaacag gttccttttt
     5461 cagacgaccg atcagatacc tggccgatgg tctgatccag aacatccttg catcagggat
     5521 gaaggcattg gctcttaccc acatgcctaa cctggaaaag aaaccacctt tttcggttgt
     5581 atccatcagc ccgtggttga ccgccacttc cctcttccct accagctttc tgtaaagatc
     5641 atatggttcg cggataggtg tgcgccatat ttcctgatcc tgtgaaacgt cctgcaagag
     5701 gctctcatct gtaatgctca ggtcaggatt ttctggtgta taaatgatcg gtttccaccc
     5761 aaaatccggt agatatcgtg catgtttcag ccagcgctgt acacctgcgc cacctacagg
     5821 aggccagtaa taagatatga tgagtacccg cttcatgtat cgtggtaaag ttgacgtttt
     5881 caggtggctt gtgcaaagcg ttcattacat gtcaatgtgt aacttagccg gttcaagcaa
     5941 ctactatgtc cggtacgaat tttcgccaaa ccgctattga atggggtatc gccatcctga
     6001 tcttcctggc tctcagcatc gcatttttca cacctgtttt tcagggcaag atcctgatac
     6061 agacagatgc gctgaatttc aaagccatga gcaaagagac cctggactat aacgccagcc
     6121 atgacgatgc cgcgttgtgg accggaacga tgttcggcgg tatgcccacc ttcctgatac
     6181 atctggaaca cagtggcaac ctgttcgcct ttctgaacaa ggccatcggg ttatggctac
     6241 ccaggcctgc caattatctg ttcatgagtt gcctgacgtt tttcatactg ctgcggtcat
     6301 tcaaagtgcg aaactggctt gccatattcg gtgccatcag tttcgccata ggcacctata
     6361 tgatttcatt cattgaagcc gggcacaata ccaaggtgca tgcctttgcc attatgccgc
     6421 tggtgctggc cggggtgaat tatttgttca ggggaaagac ctggctggga ttttcagtag
     6481 cacttctggg aatgtgcctg gagatcgacg ccaatcacat tcagatcact tattatatgt
     6541 attttctttt gttttgctgg tttgcagctg aactggtatt tgcatacaga gaaaaaaaga
     6601 tggtctctta ctggaaagtt ttcggcttgt ttgcgctgat cagtgtgctc agctttaccg
     6661 ccaatttttc ccgactttat accacctatg tgtatagcga agaaagtatc cgcggtggct
     6721 cagaactgac caaagaaggt gatctcaatg ccaattatgc cggaaacgga ttatcaaaag
     6781 actatgtgtt tgcctggagt tatggtatgg atgaatccat gaccctgttg tttcccaact
     6841 tcgccggtca ggcaagcgga aaatcatttc tggaagacga agacagtaaa tcaatggctt
     6901 atatccgtca actgtatgca cggcagccag ataaggcaca acaaatgcag caatttaccg
     6961 gcaaatactg ggggcccctg caattcacct ccggacctgt ttacctcggt gctgttgtgg
     7021 catttctgtt tctgcttggt gcatttgcag ctgagaacag gatgcgctgg tggcttatag
     7081 ccgggagtgt cgtttctctc atgcttggct ggggcgatca cttcaaagcc ttcaactact
     7141 ttatgtacga tcattttcca atgtacaaca aattcagaac ggtcatgatg gcactgatca
     7201 ttttctgtct gaccgcccct ttactaggct tttacatttt ggaaaaatac atgcgtgaag
     7261 gagacacagc acaaaaattc aaagcctgga aatatgctct gatcgtgatc ggtgtgttga
     7321 ccctgctggt actcattcct gtatttgacc caagcggacc aagagatgaa caactgatgt
     7381 ctgcctacgg gcaggatccg gacattgccg gactggtcag tgccgtacat gcagaccggg
     7441 tgtctatgat cagaatggat ggactgcgca cactggtctt catcgccctg agtgcaggtc
     7501 ttatatggtt gtatctgaaa ggcagagtga aagctattgc tgcagtgctg ggaataggtt
     7561 tactgagtgt gatcgatctg tggactgtca acagcatcta cctgaatgca gacaactacg
     7621 attcacctga ttactatgca agatattttg catcacgcct tcccgtcatc aacgatgaag
     7681 acccgaatta ccgggttctg aacaccaccg ttcgctttga tcaggatggg gtaacgccct
     7741 ggtcatacca cagtataggc ggataccatg cagccaagct gcgccgctat caggacgtta
     7801 tagacggata cctgaacaat ggcgctatga atgtcatcaa tatgctgaac gccaaatatg
     7861 tcatcgtcag caataacgga caattggcaa ctcaacgaaa tccgggtgcc tgtggtaatg
     7921 cctggctggt agattcagtt gtttttgtga acggacctga tgctgagttt gaagcattga
     7981 aagagctgga acccaggcgc tatgcggtag tagatgagca attcaaagat cagctgcagc
     8041 aggttcctga aagagattcg accgcaggca tcatcatgct gaattattcg cccaatgaga
     8101 tcacctacaa attcaaaggc aacagtgcac agatagcagt attcagtgag atattttatc
     8161 gtggtaacga agactggaaa gcttacatag atggagaata ccaggatcat ttcagggccg
     8221 attatctgct gcgtggcatg ctgattcctg ccggcgaaca tgacattact ttcaggttcg
     8281 agccgtcttc atactataca ggcagaaaaa tttctctcgg agccagcagc atcctgttat
     8341 tgctcatagc tgccggattc tggatgtcat ggagaaaaga tagagaaaac ggttctgcta
     8401 ctgaatgatc agatcttcag gcatgaccga acgaatggcg ttgtctcttg cccaggccag
     8461 cacatcttta tacacctgca gccagccggg ttctgcaagg tcaaagaaat aattgttgtg
     8521 ccacagaata ttgatgaccg catcctgccg gtgtttatcc aacaggttta tgatctcctt
     8581 tcctgctgtg gcagggtctt tcttcagata atgacgatag gtagtgtcca tgacaagaag
     8641 aggaacctca gtcagcgcta tttctcttct tcctgaaaca tcgaaaggcc tgtaaggcag
     8701 accgtatcct gccctgaatc cgggttgttc ggcaaagccc agtgtagcgt ccaatgccac
     8761 ccctgcctgc tccatgaccc gccaggtatc aggcataaag aatctcagat agtggttccg
     8821 gttgctgatc gcattgttgg tatccagccg ctccagctct tctgaataag tggtttgtaa
     8881 agcactttta tgcaggccat ttgatccgcc tgcagcagac accagttgca tcttctttct
     8941 gatggcagga tcatcaatat ggtaatcagc attctctatt ccatttcttc cgcggcctct
     9001 ttcagtaagc cagaagaata cagaacgcac atcatatgca tcttctatag ccaggatctt
     9061 ttccagttcc agatagtcat agcgaccggt aatatgcctg aacatcagtt gcagcagggt
     9121 gcctaccctg ccctttttta acaaggcttt tcgctgttcg gtccaggctc cgaacacggt
     9181 atcgatatcg tgtgtgcaga ataatcggga agcatggtct tgcttcaaga tcttctgctg
     9241 caccaacggc agagacttcg ttaattggtc aaagtgaaat gccacaagat tcttatctgc
     9301 agcatcgaac cggtattgcc agctgtctgc aaaggcaaac ctgccgtact tgtcaccttc
     9361 aaagggcgca tattcctgct ggcacgacag catataaaaa gctgtgctca gatgatctgt
     9421 ctgccccccg gcatgcaggt gtaccggtgc ggttgcaaaa taggccttcg catgatgatc
     9481 gccatgcaga taggtattcc ggaaaaaatg agataagcgt atgtcgctga tacccagttc
     9541 agagaccgtc agttcaggtt ccgtttccac gaacaatgga gtgaaacctg agtacctgcc
     9601 ccatgcacgg atgatccagg catattcagc ctgcatgtcc gtttccagct gaaagcggat
     9661 ggttggcagc atgggtcaaa actaaatgaa aacaggcagg aaaaccgaag cctcctgcct
     9721 gttcaagtta tttcatgatc actcagagat cgaatttgat gccctgggcc agcggaagtt
     9781 ctgtagagta attgatggta ttggtttgac ggcgcatata agctttccag ctgtcgctgc
     9841 cgctttctct tcctccaccg gtctcttttt cgccaccaaa agcaccacct atctcagctc
     9901 cggatgtacc gatattcacg ttcgcgatac cacaatcact tccggcgtgc gacaggaaac
     9961 gctctgcttc ctgcaggttg ccggtcatga tggcactgga aagcccttgc ggcacaccat
    10021 tctgaagctc aatagcctcc tcaatggtct tgtatctgat cagatacaat atcggcgcaa
    10081 aggtctcatc ctgcacgatc tggtagtgat tttccacttc agctatacag ggcttcacat
    10141 agcatccgct ctcataaccc tcaccttcca gcacgccacc ttccacagca aatcttcccc
    10201 cctcttcctt cactttttct atcgctgcca ggtagtcttt cacagctccg gtatcaatga
    10261 gcggacctac atgattatgc tgatccagcg gatcaccgat cttcaactgt gcataggcac
    10321 gggtcatttt atccttcact gcatcataga ccgattcgtg aacaataagc cggcgggtag
    10381 aagtacaacg ttgcccggcg gtacccacag caccgaaaac agcacctctc accaccatat
    10441 caatatctgc atgttcagaa acaataatgg cattgttgcc cccaagttcc tgaatgaccc
    10501 ggccaagacg ttcgccaaca attccaccta tgcgttttcc cacacgggta gaacctgtaa
    10561 tggatatcag gggaacacgt ttgtctttgg ccatttcttc accgatggct gaatgagata
    10621 ccatcaggca ggaaactcct tccgggatat tatttctttc gaacacacgg ctgatgatct
    10681 gctggcaggc tatggcagaa agtggcactt tggaacttgg tttccatata cacacatctc
    10741 cgcaaaccca ggcaatggca gcattccagc tccacactgc cactggaaaa ttaaaggctg
    10801 aaatgatacc caccactcca agcggatgcc attgttcgta catacggtga agcggacgtt
    10861 cactgtgcat gctcagacca tacaactggc gagataaacc cacagcaaaa tcgcagatat
    10921 ctatcatctc ctgtacttca cccatacctt cctgcaaact cttgcccatt tcatagctta
    10981 ccagtttgcc caatggctcc ttgtatttac gcaactcatc tccaacctgc ctgacgatct
    11041 cgccacgttt tggcgcaggc atcatccgcc agattttgaa agcttctgta gcctgttgca
    11101 tgagctcttc atactcttct ctgcttgctg aagtacaagt ggctatcagt tttccgtcaa
    11161 ccggcgaata agactcaaca gtcttgcctg atccggtcag ccagcgggta ccggtagata
    11221 ctccggagtt tgtttctttg attcccagcg cttccagttc ggccgcaatt ggattatgtg
    11281 tcataacagt ttccatatct cttatttcag actgcaaagt tagctattca gccacggagc
    11341 ttctgtgtgc atcagcacat ttcaggactg tttcaatgcg tttcgtattt tgcagcatga
    11401 aacagatcct gttcgccgct acacttttga ttgcccattg cggattttct cagcatgtac
    11461 ttgagctgct ggaagaacaa catcctgcgg cgggaaaggt agaagacttt tcctggatgg
    11521 cgggaagctg gtcttgtgct atctgggatg gtgtggccga agaatactgg atggttccac
    11581 aatccaacag catggccgga atgtttcgtt tttctgccga tgactccctg cgttttctgg
    11641 agatctttac ccttgaacaa tcaggtcgat cggttacgtt aaagctgaaa catttcgatg
    11701 ccggcttgca gggatgggaa gaacaggacg actgtgtcag cttcgtactg gtgtatcagg
    11761 agcccaacag agcctatttt gatggcatga cctatatcct gagagatcct gatcacctgg
    11821 atgtctttgt gctgatggat cacggcgatg gaacgcaaga ggaactccat tttccatatt
    11881 tccgaatcag ccgataaaga acccctttgt taacaaattg ataaattctg gtgctgttaa
    11941 gatttttttt tgtgaatatt gcattccatg taccatacgt tctttcgaca caggaacgga
    12001 acagtactcc tcctcgtgtc gctgctattc cttgcttcct gccagaaaga ccggtcgctg
    12061 ggcgaacaga tcaccctaca ggagctcgtt tctgaaaatc ttcagatcga actaaaccct
    12121 aacggcaata caccgttagc agcccggtta tcctttacag cagatgtgcc ggtacaggtt
    12181 tcctatacca tagaaggcga agaaccgatc agccatactt ttcctggttt ctccgctacc
    12241 caacgggttc cgatacttgg tttgtatcct gacaccgaca atgaggtcaa ggtaacgatc
    12301 tctgatcagg caggaaattt tgcttcagca accctgcatg tggaaacagg cccgctaccc
    12361 gattattttc ctgccatcga aaccaatgtg aacacctccc tccgacagga aggctggact
    12421 ttaacagaga tcggaatagg tctgggtgag gcattcatga gcgttccttc catcatcgat
    12481 gccagcggca acgtgcgatg gtatatcgtg ctggatgctt atacagatct gtgcttcccc
    12541 atcaagcaaa aatccaatgg caacctgctc atttcctttt atgagcacat atatgagtac
    12601 accatgctgg gaggtgtggt caatgacatc taccttcccg gatggtacca gcaccatgag
    12661 acccgcgaaa tgcccaatgg caacttgctg gtaagtgttg ataagatcgg agaatctacc
    12721 gtagaagacc acatcctgga agtaactcct tcgggtagta ttgtgcaggt atgggatatg
    12781 cgccctattc ttgacatgga cagatatgat ctggtggaag acgaggttga ctggtttcat
    12841 aataatgccg tgtattacag cgaagccgat gattgtttac tggtctcagg caggaaccag
    12901 ggggtggtca aagtgaaccg caacaatgag atcgtctgga tactttcgcc acaccaggga
    12961 tggggtactg ccaccaacgg taccaacacc caggatttcc tgcttactgc cattgatgcc
    13021 aacggagatc cgtatccgga ggaagtacaa cagggcacag caccggcaga tgattttgac
    13081 tggtgctggg ggcagcacgc ctgtatgttg ctgccaaatg gaaatatctt cctgtttgac
    13141 aatggattca acagacaatt catagccggt gtacccagtt acagcagggg agtagagtac
    13201 aggatcaacg aagagaacat gacggttcag gagatctggc aatatggtaa ggaacgcggg
    13261 ccggaatgct gggcgcccat catcagcgat gtggacgtgc tggataatac caatcggctg
    13321 atcacgtcag gtatcgtttt cggtgctgaa ccctacgcca agatcatgga ggtaagctat
    13381 ccggatagaa cggtggtcta tgagatcacc ctgcatttta aaaatctgct ggcagccgga
    13441 ggccttgcat ggggtgggct ggatctgaat tacagggcag agcgtatcca tatctttcca
    13501 caataaaaaa ggcggccgga gccgcccttt tcaagtatca gctatctcag aactccagtt
    13561 ttcgcatcag ctcaaatgga ttgatcccat tgaagttgac aaggaaactc acatggctca
    13621 tgtacgtgcc atagtctgca ttgaacggat ccaccaggcc atctgaagcc agcagcggga
    13681 aatacacttc gatcacatta ttcagcagtc tgatcatgac acctgcatca tactgaaaac
    13741 cggaaatgct ggccggcaga tcgccgggca cataggccgc atcagcaaag aatgcaagcg
    13801 gtagtttggg tacaggaaat tccagattaa gtgccagtaa cacatcatcc tgcaaaccga
    13861 gagcaatggt atctgcaggc aatttcagga aaccatctct tcttgtgatc tggttggacc
    13921 acatgccgtc tgtagcattt ctgcccggca ggatatcatc atacagataa tcgttatagc
    13981 tgcttactcc cgtcagggaa taggcatagc ggctgtatgg atcttctttg aagtaactca
    14041 tccatcctgc aaacagacgt gcttcaattt catactttct gatatgaata ccggtggtca
    14101 catcggcaaa cagcttgatc agatcaggac cttgttgtgc tgtcagggta gcctgatatg
    14161 gatgttgcag atgcctgttc tttacagtgt aactcagctc attgaaccag aacgctgtat
    14221 ccagtttgta ttgctctccg ttctccagaa tgcccagcac ataagtttcg cctatgcgga
    14281 cgtttctgta ccggatactt tgctttacag gatctttcag ctgttttttc cgaatatcta
    14341 tatctatggc aggctggagt ctggtgtagt aactttcagc cagagagtca acatgataag
    14401 aagctcctga taccgataat ctgacccggc tgaaggcccc tctttcagga tatatattcc
    14461 accccaggct tccctgtccg gagataccct gagcccggaa gctgtataat ggtgcagcta
    14521 cccattccag tttcggagca ggaaaagtgc tgttccacag gccaagacct accatccatc
    14581 cgtcattgac attgaaaccc accagcggca gataagagat ggttgtacga tccggattat
    14641 caggagagaa gaaaggcttc aaagccagag gctccacttt cttgaaaagg ccggaaaccc
    14701 tcatcgtatt gttttctctg ttgttgtcag gaatagtatt gtgggcgtct atgcgcactt
    14761 tggtgatctc ttcggaagaa ctcagtttct ttacatagat ggtctgtgta ccggtaaaac
    14821 cacgatgcca ttctgtgcga agaatactat caccacgcat tactgaaacc ggaaacggac
    14881 cggctatatc tgtattgttg tgcacccgaa ctgccatggt tgtctggccg tccagtatct
    14941 cagtgatctt atagtccaga gaacgatcgt ctttcagcaa gccctcaaag aaccaggcca
    15001 tattttcacc tgtttcctcc tcaaatacac gcctgatatc ttccggctgg gggtgtttgt
    15061 tattccattc ttcgaaatac tttcccatga cacggtcaaa ctggccctgc cccagaaaat
    15121 ctgccatata acgcatcagg tatgccgttt tcatatagac cagcaaacca tagttcagct
    15181 gagtaaaatt gttggagtgc tggtctattg cctgagactt attcatcctt tccagaccca
    15241 gtatcaggta ccaggccata tccagggtgt tctttccctc cagcgcatct gcatttatga
    15301 acctggcagc atttcctaaa aatggttcaa gtggattggc attcggataa taagttgtca
    15361 tatacctgtt ctcgtaatat gaattgatgc cttcatccat ccaggcatga tcacgctcat
    15421 tgcttcccag catgccatag aaccagttat gtccaacttc atgtgtgatc acattatcca
    15481 ggccggctgc attggcagaa ccgccaataa tggtgatggt cgggtactcc atcccggctc
    15541 cggcactcag tgccccttct actgcctgta ccacatccca ttggtactct cctgcatagt
    15601 tactgtatgc tataaccgat ctgttcagat aatcgatact cttcatccac agcccagtct
    15661 gaaagttggt gaagtaagac cagctgtcaa cggtacggcc gctgtatggc aactgtgcct
    15721 ggccatgcaa tacatgaaag cgtttatcag caaaccaggc gaagtcatga gcgtctttca
    15781 gttcgtactc cagtgttttg gtggtagcgg cacttggcgg aaaactgttg tcgctgctaa
    15841 agttttccat ggctgcagtc tcagctgcct tttgcaacag gaaattccgc tcactttcag
    15901 taagcaggtt tccacttgcc gccacgatat aattggatgg aaccgtgatg gtcacatggt
    15961 agttaccaaa ctcagaataa aactccccct gattcagata aggaatggca tgccagccgg
    16021 attggtcaaa aacagcaggt tttgggtacc actgggtgat ctggtaactg tttccgctat
    16081 gccccaggcg ggagaaatct gaagaaggaa tcttgacccg gaagggagtg gctatggtca
    16141 cagatgcccc ggactgcagc ggttcaggca acatcaacct ggcaatatcg cggtgctccg
    16201 gatgatattc ccaggtgact gactttcctc ctacctgaaa attaagctca tcaatgaagc
    16261 ccagttcttc tgcagaggcc agcaacatct cccggttccg gtcatcaatg aactgctgcc
    16321 caagagctgt atgggcattt tcatatgcgt ttggccagat atgcatccag atttcggtga
    16381 gactttccgg actattgttg gtatatatca tgctgatatt cccgttcagc atatgcatgg
    16441 aatcatttaa tgctacgctg atatcgtaat ccacatgttg ctgccaataa cttggtttct
    16501 gcgcttcagt ggtaatcact actttcagta tcagggcaga aaaaagaaat aatctcgtca
    16561 tgcagtcatt ttttttcaaa aatagtgatt tgaagacgca gaagcgggct tgggaattaa
    16621 tctgtctttc tttcgttagt atggtatgta cagaacctat tttgttaacc taactttgca
    16681 ggcctatgct gagaccggag caggatgatc atgctgaaga agccggatta caggagctga
    16741 tcgaacaatt tgaagctttt ctggaaaaga aggagtattc cttctttgac gatgatgctc
    16801 tggaaagact ctatgagtac tacgaaaaca ggcttgatga aggccatatg gaagccgtag
    16861 cagacctggc tatagctcaa aacccatatt caggtgattt tctggtgcgc aaggctgaac
    16921 tgctgtttaa ccgaaaagat tatcaggcaa gcctggaact gctggataaa gcagttgttt
    16981 ttgacgccac caacctcgat atctacctgt tgcgcagtga gatcatggtt gaatccaacc
    17041 ggatcgaaga agccctcgaa ctactccagg atgctctaag gctggcagac cgtgatgaaa
    17101 aggatgtgat cctgacagag atcagtgatg tgttcgaaat acaggaagat tttcatgcgg
    17161 cctttgactg cctccgcgat gccataaaac acaatccttc aaatgaagca gccttgatga
    17221 agatcgctca cctggctgaa atgacagaca ggtatgacga tagtctggac atccacctgg
    17281 ccataactga agagcatcct tacaactgga tggcctggta caatgccgga cgggcgtatg
    17341 ccggccttgg gctgtatgaa aaagcccttg atgcctttga atttactatg gccattcagg
    17401 aagattttga cctggtgtac cgcgatgtag ctgatattta tttccgtctg gaagatcttg
    17461 ttaaggccat ctccacattc gaaaccgccc aggaaaagtc aggtggcttt gaagattaca
    17521 gcttccgtat cggtatgtgt tatgaaagaa agaatgacct gaaaaaggcc cgctttcact
    17581 atcgaaaatc cacccgccag gatccttatc tggacgaggc atttttcagg atcgcagaaa
    17641 cctaccgtca ggaagaccgc tttgaaccgg cattggtcaa ttacaagaaa gcactgcgca
    17701 tagatgagac caatgaactg tacatgacct gcatcattgc tgtgtatcgc atgctgggcc
    17761 gcgaggaaga agtattacaa tacctgcaaa ggcttacaca ggccaggccg gatattctga
    17821 actactggct ggatctgctc atcttccgat gggaacatga acaatttgaa gcaggtctgg
    17881 aaaatgttca ggaagccacc gaacgctgcg gtcactttgt tgagtttgat tatctcaggt
    17941 cattgctttt atggtatgcc gggcagcagc aggaaagcct gagggtactg gcacaagccc
    18001 tggatacaga caaggcaaga cacaccattc tactggaaac agatccgtcg tttcttctga
    18061 aagaagctgt catgcgggtc atgaatgctc aatgagcatc gttacgggct tcagcaacaa
    18121 agaaaagttc tgtattcatt ctgggctgta ctgagttttc gcagggttcc atcagcagta
    18181 tgtgaaacca gggtttcatc agttttctat agtctgattc ttctcccccg aaaggaggcc
    18241 cctctttttc aaactctaca ttgaacagta ctcctgccaa gtgccctccc ggtttcagca
    18301 ggctgtgcat atgctgcatg taatcttgcc taagagccgg atccaatgcg caaaaaaatg
    18361 tctgctcaag aatcagttga tactgcccct gatgatcaaa aaagttactt tgtatcgtct
    18421 tcaatcctga tcgatcaccc actctgctgc gaagccgctc cagaggaaga gctgcaatat
    18481 ctaccacggt tacatcgcta aatccctgct ccagcatata ttctgcttca tagccgtggc
    18541 cgcagcccgg aatcaaaact ggagcatact tatcctgtaa atgatcaaca taggccttta
    18601 ttggcggcga taccgcaccg agatcccaac cggtctggtc attggcatac cggtgttccc
    18661 aaaaagtctt gttcaacata gcggtctttt gaggccaata tacccctgtt tcgtaacttt
    18721 gccggtgaca tttatccatt atgaactttg aattgaacca acttccggaa cgcagcagca
    18781 agccacgcac caatggcctt acaatggtca tggacaaggg tcttagtatc cgcgaagccg
    18841 aagatatgct gagcgtatcc ggcccctatg ttgacatagt caaactgggt ttcggaactt
    18901 cctatgtgac ccctaatctg gaagagaaga tcaaattata ccagcatgcc ggactgggtg
    18961 tttacctggg tggaaccctg ctggaagcat ttatcatccg caacaggtac aacgattttt
    19021 tgcaattact tgatcgctac ggattgcagc atgtggaagt gagcgatggt tccattcaga
    19081 tggatcatga tgaaaaatgc agattgatca gagaactgtc ttccagggct acggtactga
    19141 gcgaggtagg gagtaaggat gccaagaaag aactggctcc ttatctgtgg atagaactca
    19201 tgcaaaaaga aatagatgcc ggttcctgga aggttatcgc tgaagccaga gaaagtggaa
    19261 ccgtgggtat ttatcgcgac aagggagaag ttcgagaagg actgatcgat gagatcctta
    19321 caaagattcc acaagataag atcatgtggg aagcgcctat gaaatctcag caggtgtggt
    19381 tcatcaaact acttggcgcc aacgtcaacc tgggcaatat cgcaccaaat gaggtcatcg
    19441 cactggaaac gctgcgtatc ggtttgcgtg gagatacctt tcatcatttc ctgaagtgat
    19501 gtcggtgttc aggaagtatt tccctgtcac cctgaaaggg atggccatgg gagcggcaga
    19561 cgtggtacca ggtgtatccg gaggaaccat cgcatttatt tcaggtatct atgcagagtt
    19621 gctgaacagc cttcgttctg tcaacctgaa ggcattgcgc atattgttca gggaaggaat
    19681 tcctgctttc tggaaacata tcaacggttg gttcctgctt ttcctgctga caggtatcgg
    19741 tatcagtatt gtcagtctgt ccaggatgat cctgtatctg ttggagaatt atccgcctct
    19801 gatctggtca ttcttttttg gtcttgttgt tgcctcagcc atctacgtgg caggtaccat
    19861 caaagaacga aactggaaag tggtgctgtc tggccttgcc ggaatagtca tcgccggatg
    19921 gatcaccatc gcatcgccgg ctcagggttc agattcgctg ccctttgttt ttgttacagg
    19981 tgccattgct atttgtgcca tgatcctccc cggcatctca ggcagtttca tcctcctgct
    20041 catggggatg tatgaattta ttttaggtgc attgcatgac ctgaagatta gcgtgatcat
    20101 cacatttatg gcaggcgcag gcatagggct ggtatcattt tcgcatctgc tctcctggct
    20161 gctgaagaaa tatcatgacc tgacggtagc attgctggca ggctttatga tcggctcact
    20221 gaataaagtc tggccctgga aggaagtgct ggaatccagg ttcattgacg gagaagaaaa
    20281 ggtcattcag gaagccaatc ggcttcccgc aacatactca gaggttacag gcgagccaaa
    20341 tcatttgtta ttttgtatac tgctgttcct gaccggattt gttttgatat attcgttaga
    20401 gaaatttgcc ggtaagaaac atcaactcat ctgattttta tggatagtgc atctgaccct
    20461 tacccgaacg atgatcacgc ctcgctgcag gatgaacacc tgaaactcta tggcctcata
    20521 ggaaaatctc tgacccattc cttttctcag aagtatttcc tcagcaagtt caaaaatgaa
    20581 gagctggagg atcactacta tgcacttttt ccgctggacg atatagatga cattcgcttg
    20641 ctgctggagg tgcacaagaa tctgaaaggg ctgaatgtga ccattccgta taaggaatct
    20701 gtaatcccct atcttgatga actggatccg gtagcggctg agatcggggc ggttaactgc
    20761 atctatatgg atgactttga gaaaaagggt tacaatactg actggctcgg ttttcgtgac
    20821 agtatctttc cattgcttcg tgctgctgat aaaaatgcgc ttgtgttggg tgccggagga
    20881 tcatctaaag cggtgtgcta tgctttgaaa caacttggtc tatcgtataa ggtggtatca
    20941 cgtgaggccg gaaaatctga tctgacttgg gatatgctga ccactgaggt catagctcag
    21001 cataccgtta tcatcaatac tacgcctgtg ggcatgtggc ctgatgcgga tgctatgcca
    21061 cctgtgccaa ttgatgccat aagcccgcag cacatctgct ttgatctgat ctacaatccg
    21121 gaacaaacca gattcttgca ggaagccgct tccagagggg cggttacaag caacggtcaa
    21181 caaatgcttg aattgcaggc ggcacatgcc tggaagatct ggaatacccc aatagaggaa
    21241 aaagaagagt aaggtcagcg cttttcctta aaaaaacagc ttccgggttt gggacgaatc
    21301 acctggtcaa aatgataggt aatggtaaac agacctccca acacataagc atcacggttc
    21361 ttaggattac cacgtcctgt ttccaaagag atcagatccg gcctggtgtg gtcagacatg
    21421 tcagataatg ctgccaccat ggtagcagtt gcagggtcat aaaatccaaa gaaataatcc
    21481 ggattgggat aactgttact tacatcatcc atataatctg acaaggatag ccggctggct
    21541 gtttccatgc caaataccca gttgcttctg gtaagatact tgatgccccc gccaaaggga
    21601 aacacaaacg tgtatttggc atatggctgc ctatcaggat actggggcag cccctgtcct
    21661 tcagtaccta atggttgtaa ctccacccat tgaccctcat acaatgcttg tggattaaac
    21721 cagcacatac ctgctccggc atacacaaat ggtgtgaagc gctctctgtt ggggcccgtt
    21781 aaacgccgga gtgtataatc cacatacagg caaacttctg ttagcggcga cttaaaactg
    21841 agattcctgt agcttcgaaa atcattttct gagagtgaat cagcaccgcg tatctgcgta
    21901 taataagcat tcagtctgac actgatatgc ggcactactt cctgcctgta gatcaccccg
    21961 atagccggcc tggtcacttc ggggtcaata tcccaccata aagcccgtcc ttccattctg
    22021 gagcctccca gatcacccag aaagttggag aagcccaaat tgatacccac ctccgcatcg
    22081 cggtatttgg tctgaccaga gcatactccg gtagccagca gaattactgc caaaacaggg
    22141 aggtggcgct tcatgttaga acaaaactaa tgcaatctgt cagacttctg cactatgcca
    22201 ggagcaactt ctacattaat tgacggaatg cctgcatgcc aacactacct gatctataat
    22261 aaactgttga tcctgaacac ttaatgtgtg ataaagtggt aacctgacaa gggtatctga
    22321 ccatttttca gccaggttac atgttttgtc tgacctccat ttttgtccca ttttggagct
    22381 atgaagcggc aaataatgaa aaacagccat gattcccgca cgttgcagag cgctgatcag
    22441 ataatttcga acctgtctgt cggggcactg aacatagaag atacttccat tgtgatgcac
    22501 attttcagga ataacgggaa gcgcaaaccc atctggcagc acatgctgaa atgaaagata
    22561 atagcgattc cactgctcag ctctcttgtt cagaatgctt tcggcttctt tcagttgagc
    22621 cattagccag gctgcctgaa tttctgatgg aagaaaagaa gagcctatat ccacccattc
    22681 ataatgatca gactgacctc tctggaaagc tgctctgttg gtacctttct cccagatgat
    22741 ctcagcacgg ctcatcagat cacttctttg caaagccagt gctccgcctt ctccgcagcc
    22801 taatactttt gtttcatgga atgaaaatgc tgtaagtggc gacaggctac ctacaggttt
    22861 attgttaaaa gtgcttccca gtgccatggc ggcatcttct accaccagaa tgttgtgctg
    22921 cccggccagc ctgttgatcg catccatatt acagggtacc ccggcatagt gggtcaccac
    22981 gatcacgctc gtcctttcag taatcagatg ggctatggtg tctgcagata cgttggggtg
    23041 aagatctgta ctatcagcaa aaacaggtac agccccccgc tgcacaaaag cattggcagt
    23101 agatggaaat gtaaaggaag gaacgatcac ctcatcgcca tgacctacgc ctgacaatat
    23161 tgcagccatg tcaagtgcag tgctgcagga tgtggtgagc aacacatttg atgcacctgt
    23221 gatattctga agcctgttgc agaccgatct ggtcatgatg ccatttcctg ataacctgcc
    23281 tgaacgaaca gcctgctcca gatagtcctg ctcaagtcct gttacatatg gcttattgaa
    23341 tggtatcatt catcaaactt agtgtaaatg atggtctcgg tctggtatgg aagggtcttg
    23401 ttaccggaac ggtgaaagag tgcataagat aaggcctgaa gtgccagctt tagctgtctg
    23461 ctgaaggtat aggtactact cccgtgcttc cgctctccat gcctgaccac aagtaccggc
    23521 aaatgaccac catgtattct tacagccgcg ggaaagtaaa aattgcggac accatcctgc
    23581 agaatggat
//
LOCUS       DAOMAX010000009        22943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_230_length_22943_cov_4.275122, whole genome shotgun sequence.
ACCESSION   DAOMAX010000009 DAOMAX010000000
VERSION     DAOMAX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230_length_22943_cov_4.275122"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK742_01265"
     CDS             complement(<1..259)
                     /locus_tag="PK742_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQU75245.1"
                     /translation="MPFYHTLGAIPPKRHIQYRKPDGSLYHEELFSTEGFSNVYSLLY
                     HLYPPTRIRQVGEPFSVEPKVVLSEQLKPRSLKGFNIQPEDD"
     gene            complement(349..1527)
                     /locus_tag="PK742_01270"
     CDS             complement(349..1527)
                     /locus_tag="PK742_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HQU75246.1"
                     /translation="MEPNQKISFDDTSIAFSSKNDAALKREYRLFRLMNNGNLVRIGT
                     RLATLALRMHLPVRGLIRKTIYQQFCGGETIRQTGKVLEALHHSGVHAILDYGVEGKE
                     NEEDFQRTTDQLIQTIRFAGTQPNAPFISCKVTGITRFDLLEKLSAQQPLSTTDQADR
                     DKLFERMHQIASTAKDAGIGLYIDAEETWIQDAIDQLTFELMRTYNKDRAVVYATPQM
                     YRHDRLNWCKKAYEDAVTNNYILGLKPVRGAYMDKERRRAAELGYPSPIQPDKVSTDR
                     DYDAVLAFALDHLDKVAVCAATHNEKSSMFLATEAIKRGIEPGNSHIHFSQLYGMSDH
                     ITFNLAKAGFNASKYLPYGPVKDVIPYLIRRAQENTSVAGSMSRELSLINKEIKRRGL
                     "
     gene            1612..2895
                     /locus_tag="PK742_01275"
     CDS             1612..2895
                     /locus_tag="PK742_01275"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQU75247.1"
                     /translation="MKMLLISGLMIASSVMGQSYTSYLTGDSSDVVTNPAPGLVLMGG
                     GGENDQAMTWFLNRADGGDVLVIRASGADGYNDYLFSELGVSVNSVETIVFNEASAAE
                     DPYVLSRITQAEALWIAGGDQWDYISYWKDNAVEDAINYLIEEKKITVGGISAGMAIM
                     GDAYFSAQTGTVTSDEALNNPYDEQVQLGWNDFIRNPAMTGTITDTHFDSPDRKGRLI
                     TFMARLFTDYGFLPVGIACEEYTAVCIDTAGIATVYGEYPDYEDLVYFLQVNCQEPNS
                     PQQCIPGQPLQWQLDELTVHATVLAGTPSGTSSIDLYDMENITGPVFAFQNWQVNSGT
                     LEVLPFEEAYTCDSLISYVNEMILPVSLYPNPASEAIVFQAAPGIYRIFRADGQEVEQ
                     FYSDGSEVLLQISLYPAGYYVISGQGFACPFMKFD"
     gene            3015..3485
                     /locus_tag="PK742_01280"
     CDS             3015..3485
                     /locus_tag="PK742_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HQU75248.1"
                     /translation="MKLQLWKLVLSVLLCITIGAIGGFFTSGEIDTWYAALEKPPLNP
                     PDWIFAPVWTTLYILMGISLALYWSAEYGNSKRTGFMVFLIQLGLNLLWSLLFFKLHS
                     PLLALIDIVCLIGFILTNMYFFARVSRQSALLLLPYLAWVSFATYLNLSVLQLN"
     gene            3517..5253
                     /gene="dnaX"
                     /locus_tag="PK742_01285"
     CDS             3517..5253
                     /gene="dnaX"
                     /locus_tag="PK742_01285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQU75249.1"
                     /translation="MQQFVVSARKYRPLSWDEVVGQDHIVSTLRNAIKSDHLAQSFLF
                     CGPRGVGKTTCARILAKTINCENLSPEGDACNECESCLSFTQNASFNIHEIDAASNNS
                     VDDIRSLVDQVRYAPQSGKYKVYIIDEVHMLSAQAFNAFLKTLEEPPSYVKFILATTE
                     KYKIIPTILSRCQIFDFNRITIRDMIRQLKKICGNEGIVADDEAFHFIAQKADGAMRD
                     ALSIFDRITTFAGKNITAADVIENLNILDYDYYFKVVEMIAEENAAGILLLFNEVTAK
                     GFDGDTFLIGLAEHLRNLLVCMDPSTVSLLETSEAVAARYKEQAASADASFLLSALNV
                     LNQADIQYRVAKNKHLHVELALMKLCYIRHAVRTPGLESSPEKKTADLTNKPPVVQRK
                     EVQEELKTSPKEEEAENKPPLSRQEQEKKPESTGPVVTPSIRLGGKKKEKETTEQPEV
                     SPLEERREISPELFFMAWKQYADLIRKDKVNMAMMMDHARVKLDAGNVIQIQLDSQVE
                     VNMFRDATQDLRQFLADAMSNHQFDLEFSVVEAEKVVQRPYTIPEKYKKMEEINPAIR
                     DLKEQLGLELDI"
     gene            5264..8368
                     /locus_tag="PK742_01290"
     CDS             5264..8368
                     /locus_tag="PK742_01290"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQU75250.1"
                     /translation="MKRVQIFMMLCIVSCISLMAQTPPSSSSSTYGQQNQQVNPQNPP
                     NNQNQPQVSSGSIIVPGTVGGYTDPLQLYSTPSSSSKVNYAAKNDIPVMVFKNDPTGT
                     RMYRLQNGLTVYLSKNTLEPRIQTFIAVRAGSKNDPAETTGLAHYLEHMMFKGTDHIG
                     TTNWERERIMLESIAQLYEQHRNEKNEDKKKEIYAEIDRLSGEAAKLAIPNEYDKMIG
                     SLGAKGTNAWTSLEETVYVNDIPSAELERWLIVESERFHRLVLRLFHTELEAVYEEYN
                     MGQDRDGSKMWKEFYKQLFPTNTYGTQTTIGEGEHLKNPSMYNIINYFTTYYVPNNMA
                     ICMSGDLDYDKTIAMINKYFGGYEPRVVPAYRPKKESPITSVKRSEVWGQEAEYLFIG
                     YRLPGSNTDDLKKAKVIAELLSNGSAGLLDLDLNQQQLVLESGAFVDEMRDYAVLVLY
                     ANPKEGQSLEDCEQLLLKEMEKIRQGDYADWLMDAVIDNMRLQQTRNFESNRGRAGAH
                     VQSFILGKDWQSLVDELDVMDAFSRSQLTDFAVSNLRNNNYVVVYKRQGEDNNVVKVD
                     KPQITPVEANRESSSDFLRKFMDIPSLATEPEFVQYDKAIQTSTLESGIQFDYINNKL
                     NDLFNLYYIFDMGSDNDLLLSLAVNYLPYLGTDKYSAAEIQEMFFRYGLSFNVSTSRD
                     QTYVSLSGLEKNMEQGIALFEEILNSVKADKGKYEELVNIMLKDMDDAKSNKNAILFQ
                     ALFSYGQYGESSPFKNQLFEKQLRSVNPNDLTDKIHQLESYPHKIFYYGSADRDKTAA
                     LIDQYHVLPKKQRSIPPATAFTEQPTDKNRVLFVQYDMVQAQILRISKDEPFNSKLYP
                     AAVSFNEYFGSGLSSIVFQEIRETRALAYSAWANYTIPGKANESHYVRSYVATQADKM
                     QLAIEAMNEIMNEMPQAEEQFIQSLDASRKKIESERITRTGIYWSREAAAKRGLNYDI
                     RQDAYEALSGMSLYDLNRFFEEHIADRTYTYLVIGDKNKLDMDYLRSLGEFKELTLEE
                     IFGY"
     gene            complement(8416..8802)
                     /locus_tag="PK742_01295"
     CDS             complement(8416..8802)
                     /locus_tag="PK742_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75251.1"
                     /translation="MYRLQLILFILLLLNGLAATVGGVMMIMDPTGASVHLPLEWLKE
                     APFHNYTWPGIILLLSNGLLSLFIAWITFKGAMQYSYWALLQGFILVGFVTIECIMLG
                     QFVGWLHIPSYVIGAGLIGIGFVLQD"
     gene            8977..10236
                     /locus_tag="PK742_01300"
     CDS             8977..10236
                     /locus_tag="PK742_01300"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HQU75252.1"
                     /translation="MAEVIRMPRMSDTMTEGVFVEWHKKVGDKVKSGDVLAEVETDKA
                     TMELESYNNGVLLYIGVEAGKAAPVDSVIAIIGKEGEAFQELLDAAQKEAPAAEKSAE
                     EPVKEEPKKETPAPRPEPVQETITFQENGDGRVKASPLAKKMAAEKGIALTQVSGSGD
                     GGRIVRRDIENFVPGTETSEVAPVINIPAYIGEESFEEVAVSQMRKTIAGRLSQSKFT
                     APHFYLTISVDMSNAMDARKRMNENSPVKISFNDIIVKASALALRRHPGVNSSWLGDK
                     IRINHHVHIGVAVAINDGLVVPVIRFADGKSLSAISAETGDLAKRARDRKLQPQDWEG
                     NTFTISNLGMMGIEEFTAIINPPDACILAVGGISEVPVVKNGQIVPGHVMKMTMSCDH
                     RVVDGATGAAFLQTLKGFLENPVSMLV"
     gene            complement(10241..10534)
                     /locus_tag="PK742_01305"
     CDS             complement(10241..10534)
                     /locus_tag="PK742_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75253.1"
                     /translation="MDHHTLQHVEKEAIPALHFSRSEVLPPNPEFHDKRRQLLFQAMI
                     LGNTYHSKVRIIFETIEGLCQVETTIWATTEHYVLLKGGVHLPIWCIHDVILP"
     gene            10608..10982
                     /locus_tag="PK742_01310"
     CDS             10608..10982
                     /locus_tag="PK742_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQU75254.1"
                     /translation="MSDIFAGKTLVLGASPKPHRYANKAIRKLREYGHEVVAVAKRKD
                     TAWGVEIVTGMPDMKDIDTVTMYLGQENQKEYYSYIIALHPNRIIFNPGAENPELAAM
                     ATEAGIEVIDACTLVLLATGQY"
     gene            10984..11652
                     /locus_tag="PK742_01315"
     CDS             10984..11652
                     /locus_tag="PK742_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQU75255.1"
                     /translation="MSRPSHTVENYLKCIFSLGQGKDERIPTNAIAEKLGTTAASVTD
                     MIKKLSANGYVHYERYHGVTLTRTGRQIAVALIRRHRLWETFLVDKLGFQWDEVHDLA
                     EELEHINNDLLSERLDAFLGNPTVDPHGDPIPDKDGNIQYHEDVSLDQLEAGQQGIIV
                     GVTDHSTDFLQYLEKNGLVIRAVVKVMQVEAYDQSKRLELDKGKQLNVSHKVAANLLV
                     RKMR"
     gene            complement(11636..12481)
                     /locus_tag="PK742_01320"
     CDS             complement(11636..12481)
                     /locus_tag="PK742_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQU75256.1"
                     /translation="MSDAFWIILTGSLVAATSGLLGCFLVLRKMALVGDAISHAVLPG
                     IAIAFLISGSRSSVPMLLGAAAFGLFCTVLIEVFSKRARVQEDAAIGISFTWLFAIGV
                     ILITFFADKVDLDQECVLYGEIAFVGFETWLGIPVAVWTLGVLLIFILAMIGLGYKGL
                     FITTFDPEYATTLGISVAGWHYLLMSSVSVTTVFTFESVGAILVVAFLIIPAATAYLL
                     THKLKTMLLLATLIGITSSILGYYLALWLNASISGSMVVVLGVLFIAALAVSRGKRMQ
                     ISAFS"
     gene            complement(12481..13722)
                     /locus_tag="PK742_01325"
     CDS             complement(12481..13722)
                     /locus_tag="PK742_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HQU75257.1"
                     /translation="MRFVLAGMVLLGIASSTIGTFAFLRKRSLTGDAVAHSVLPGVCL
                     AFLIFQTRNLWVLLGGAFVTGWLSILFVDLITSKSRIKPDAAIGIALSFFFAIGIVLL
                     TYIQQTGLPGQSGLNNFLLGKAAAMAPEDIRTMSLVSIVIVGTVAVFFQPFRLLSFDP
                     DFAKATGMPVRFYETLLTTLTVLAVAAGIQAVGVVLMAAMLITPPAAARFWTNKLGVM
                     ILLSVLFGVAGGILGAYVSFANNKMPTGPWVVTMTSLLVVASILFAPGKGVFSRWWKK
                     RQYRNKVQRENLLKALYHLEEATPGKQWFGLPEIMEKRPDLSDALAGQLRQLQRNGMI
                     EQSQNKYAVTPEGMTEGKRIVRIHRLWEMYLTEKMNIASDHVHEDAEAIEHIITPELE
                     ERLAALLDYPEKDPHNKEIPW"
     gene            complement(13777..14565)
                     /locus_tag="PK742_01330"
     CDS             complement(13777..14565)
                     /locus_tag="PK742_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HQU75258.1"
                     /translation="MTTKVADPVIEVHDLTVSYQRKPALWGVDFSLPSGVLAGIIGPN
                     GAGKSTLIKAMMGLVSGDSGYVRLFNRPIDEVRGKVAYVPQRGTVDWDFPASVLDVVL
                     MGRFKKRRLFSRLSKADEALALRCLEQVGMEGFKYRQISQLSGGQQQRVFLARALAQQ
                     AEIYLMDEPFAGIDATTEQTIFELLGKLRDEGKTILVVHHDLQSAYQYFDWIILLNTQ
                     LVASGPRSDIFVPDYLRKTYGGKLNMLTSVGEMLAEMEAPTREK"
     gene            complement(14562..15488)
                     /locus_tag="PK742_01335"
     CDS             complement(14562..15488)
                     /locus_tag="PK742_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQU75259.1"
                     /translation="MRTSIFTALVCAILLQACKQPETENTDGPLIVVTTGMVADATRH
                     IAGPDARVEALMGPGVDPHLYKATQGDLVLLRKADIIIYNGLHLEGKMQEIFDQLAET
                     KPVYAMGAGIPPQNLLDANGPQQKNYDPHIWFDVGIWAGAVQKLGYFLSSNMPEHSDS
                     LILRTAAYTDTLLALHREISQTLQAVPANKRTIITSHDAFRYFGRAYQFKVVGLQGIS
                     TAAEFGLKDVTDMVDLIIAEGIPAVFVESSVSEKSIRSVMDGCAAKGHPIQLGGTLYS
                     DAMGAADTPEGSYTGMVRSNVQTLVNALTRKP"
     gene            15581..15982
                     /locus_tag="PK742_01340"
     CDS             15581..15982
                     /locus_tag="PK742_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75260.1"
                     /translation="MKNPFVRGDIRQYSKIVEDTEIAEFPSGTVHPVYSTFSLARDAE
                     WSTRLFVLEMKEADEEGIGTFVEVRHHSPALVGMEVIFTAKLESVLGHEVICSFEAHV
                     EERLIATGRTGQKILKKEKVERLFGSLGTEV"
     gene            15979..16437
                     /gene="smpB"
                     /locus_tag="PK742_01345"
     CDS             15979..16437
                     /gene="smpB"
                     /locus_tag="PK742_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQU75261.1"
                     /translation="MKPQQLNIRNKRAGFDFELIDRFTAGLALRGSEIKSVREGKASI
                     NEAYCLFKDDELWVRNMHIAEYDMATNWQHEPLRMRKLLLEKRELRKLLSKVKERGYT
                     IIPLRVFMSDRGFAKMEIALARGKKSHDKRHSIKAKDEKRDLDRSLSKFR"
     gene            complement(16447..17046)
                     /locus_tag="PK742_01350"
     CDS             complement(16447..17046)
                     /locus_tag="PK742_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75262.1"
                     /translation="MKKTRQILVSTTIIGMTLLFSSCFKKGEDDPFFSFHSRDARLAQ
                     NWMMESMQGTVVNTIDGQTITTYYDFDGTNIFINTDGATESYGYAYAIKVKDNGEVSS
                     TETRTDPANANDVLQTSTKNSYWFWSNDAQNKTTVNMDLTGLLEPYLSYDIPRLAWSD
                     MTWTVNFSDNYQQNEDTVTISTSTSVNFTINWVYDNGRN"
     gene            complement(17127..17816)
                     /locus_tag="PK742_01355"
     CDS             complement(17127..17816)
                     /locus_tag="PK742_01355"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQU75263.1"
                     /translation="MKFSSGQRFVIQMSAIDTYRHKGMRSQLVELLRSKGISDERVLH
                     AIEQVPRHVFFDKAFIEHAYEDKAFQIGSGQTISQPYTVAYQSQALQATPGMKVLEIG
                     TGSGYQAAVLAEMGLKVFSIERHRPLYEKSRKMLDSLGYRQIKTTFGDGYAGWPVFAP
                     FDRVIVTAAAPEIPPALVEQMKPGGRLVIPLGQEEDQVMVILTRQADGSVVREDGELF
                     RFVPMLPGKVF"
     gene            17914..18780
                     /locus_tag="PK742_01360"
     CDS             17914..18780
                     /locus_tag="PK742_01360"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQU75264.1"
                     /translation="MKKMLLATLALLFVTGTSVFAQQNIGLNAEADYPISPADYGLTF
                     EEIHVETPDKKEDNTNLMLFGWHLIPNDKTSRKAMIIAHNGQGNMSYALENAGIFLGM
                     GYHVFMFDYRGYGQSDAFNVSPKFYIYAQFATDLNAMVDYVKKYHTVMTCSIYGYGIG
                     GGLALGVGASNIKTYSVIADAPYITLESIEKTYKEMTGQKIFMPLAYDKAMIEPGNAL
                     PVKGDHLRGIMLICGANGDLIKAADVAALANYHKKVTTVYTVPGVNNDMNFESNKDEY
                     YKQVKSFLDGLD"
     gene            18873..20273
                     /locus_tag="PK742_01365"
     CDS             18873..20273
                     /locus_tag="PK742_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75265.1"
                     /translation="MVQIKFGTDGWRAIIAREFTNDNVARVAAATAEWAKARSEGPAV
                     VIGHDCRFAGEMFAEVTAKVLCAAGVKVYIAKGYVSTPMVSLGANKLGAFAGVVITAS
                     HNPPAYNGFKLKSGYGGPTIPEEISWVESHIPEVTEIPETDLDTWAAQGLLEWVDLEE
                     MYYQHVLASFDMDLINNSGIRLGYDAMYGAGQRIALRLLSNIVPLHVDDNPGFHGQAP
                     EPIHRNLTELSELIKGDNSIQAGLANDGDADRIGMYDEQGNFVDSHHIILLLIHYLHH
                     YKGYTGKVVIAFSVSDKVKRLCEHYGLEYEVTKIGFKYICGKMVTDDVLLGGEESGGI
                     AVKGHVPERDGIWCGLLLMEFMAQTGKNLNQLIEEVYAITGSFCFDRNDLHLTDALKN
                     SIMEKCAAGAYQSFGQYQVQRVEDIDGYKFHLDDHSWVMIRASGTEPLLRVYAEASDR
                     AATDAILEAAKKTLLG"
     gene            20353..21342
                     /locus_tag="PK742_01370"
     CDS             20353..21342
                     /locus_tag="PK742_01370"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HQU75266.1"
                     /translation="MRSILFLFLLFSFHSTITAQTENPPPLRDFLTTSEEFNKGRLGL
                     VLGTEGVLYTGAVLALYQYWYKDYPTEPFHFFNDNAEWLQQDKAGHFYTAFFETNLTT
                     GLYRWTGMEDRKAYWAGFATASALQLTIEMFDGFSEKWGFSPGDFAANTAGAALATGQ
                     YFLWDEQRIRVKFSSHFVNYSGYDQAVQDRAVALYGTSGPERVLKDYNGLNLWLSVNP
                     THFLSEDTWWPHWLMISAGYGADGLFGGFENAWCDDPLVKPEECDPAHLTSYPQIPRY
                     RQYYLSLDVDLTAIETNSPFWDMMFEILSIVKIPAPAIEFNGDGRVNFYPLYF"
     gene            complement(21333..21989)
                     /locus_tag="PK742_01375"
     CDS             complement(21333..21989)
                     /locus_tag="PK742_01375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU75267.1"
                     /translation="MKKTLKWNLAQKAELAWWQRYLKNKEVGAYHDWKRGYWQDLLAK
                     ISDACEVADGMEVLDAGCGPAGIFMALSNCSVDAMDPLLDQYADELDHFNKADHPNVR
                     FFHSPLEMYEPDRQYDIVFCMNAINHVSDIRLSYDRLVSWVKPGGKLVVSIDAHNHSF
                     FKHLFRLIPGDILHPHQFDLKEYGYFLSDRGMILVKSLRLKHEFFFDHFVQVAVRDQK
                     "
     gene            complement(22061..22693)
                     /locus_tag="PK742_01380"
     CDS             complement(22061..22693)
                     /locus_tag="PK742_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75268.1"
                     /translation="MATTLLVFESSCQPILRATIGMHSSKYLSQSEIEEYARIYHIDS
                     DNLYILDEVKYLAFVDEYTKVSGKNYFEENFLTNPLLVMGFDQTGSNQFFMRSCDAGG
                     FPNLKWNRYGYFDHHPLLMNPLYLPDTLYGLDSFQTYVQFYKPLNPSTGFVDSSCKEI
                     LVVHWNNIMGRQTKRLLKTVNTYIEVNCDNICVLYVNTDNLYARTKKQFH"
     gene            complement(22740..>22943)
                     /locus_tag="PK742_01385"
     CDS             complement(22740..>22943)
                     /locus_tag="PK742_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75269.1"
                     /translation="GDTKLLMNCSTTPDNGNGYTQILQDWTWPSEIANEFGYDSLVVK
                     QGIYAVNYNNSPNGKVTFDIEVY"
BASE COUNT         6178 a         5481 c         5585 g         5699 t
ORIGIN      
        1 aatcatcttc cggctgaatg ttgaacccct tcaggcttct gggcttcagc tgttcgctca
       61 gcaccacttt cggctcgaca gagaatggct cacctacctg cctgatcctg gtgggcgggt
      121 acagatggta cagcagtgaa tacacattgc tgaatccttc tgtgctgaac agttcttcat
      181 ggtacagact gccgtccggt ttcctgtatt gaatatggcg tttgggaggg atagctccca
      241 gcgtatgata aaatggcatg ggtacgaatt tgtacctgta aaattaaccc aatgagaagg
      301 gagggtggtg gtgatttttg tcaatgactt tttgcccctg agatgatcct acagccccct
      361 gcgcttgatc tccttgttga tcagactcag ctcgcggctc atgctgccgg ctacactggt
      421 gttctcctgt gcacgcctga tcagataggg aatgacatcc ttgacaggcc catagggaag
      481 gtacttactg gcattgaaac cagccttggc aaggttgaat gtgatgtgat cgctcatgcc
      541 atacagctgc gagaagtgga tatgtgagtt acccggttct attcctcttt tgatcgcttc
      601 cgtagcaagg aacatggagc tcttttcatt atgcgtggca gcacaaaccg ctactttatc
      661 gaggtgatca agtgcgaacg ccagcactgc atcataatcg cggtcggtgc tcaccttatc
      721 aggctgaatg ggcgaaggat atccaagctc agctgcccgc ctgcgttcct tgtccatgta
      781 ggcaccacgt accggtttca gacccagtat gtagttgttg gtcacggcat cttcgtatgc
      841 ttttttacac cagtttaacc ggtcgtgccg gtacatctgt ggagtagcat agaccacggc
      901 tcgatcttta ttataggtgc gcataagctc aaaggtgagc tggtcaatag catcctggat
      961 ccatgtttct tcagcatcaa tgtacaggcc aatgccggca tctttggctg tggaagcgat
     1021 ctggtgcatt cgctcaaaca acttatcccg gtctgcctga tcggtagtag aaagcggttg
     1081 ttgcgcactc agtttctcaa gcaaatcaaa gcgggtaatg cctgtcacct tgcaactgat
     1141 gaacggcgca ttgggctgtg tgcctgcaaa ccggatggtc tggatcaact gatcggtagt
     1201 acgttgaaaa tcttcttcgt tttcctttcc ctctacccca tagtccagaa tggcatgcac
     1261 acctgaatgg tgcagggctt ccagtacttt tcctgtctgc ctgatggttt ctccgccaca
     1321 gaactgctgg tagatcgttt tcctgatcag acctcttacc ggcagatgca tgcgcaatgc
     1381 cagtgttgcc agccgggtgc caatgcgcac cagattcccg ttgttcatca gtcggaaaag
     1441 acggtattcc cttttcaggg ctgcatcatt cttgctggag aaggcgatgg atgtatcgtc
     1501 aaaggagatc ttctgattgg gttccacctg aactggattt gcacaaaaat aatcaagtaa
     1561 agtttgtgca aagtctttaa catgtggtca aattgaaaca actttatcgg catgaaaatg
     1621 ctgttgattt caggtctcat gattgcctct tcagtaatgg gccagtctta tacttcatat
     1681 ttgacgggcg attcctcaga tgtggttacc aatcctgctc cggggctcgt tctgatgggt
     1741 ggtggtggcg aaaatgacca ggctatgacc tggtttctga accgggcaga cgggggtgat
     1801 gtcctggtga tcagggcttc aggtgccgat ggctacaacg actatctgtt cagtgaactg
     1861 ggagtctccg tgaattcggt agaaaccatt gtttttaatg aagcttctgc tgccgaagac
     1921 ccctatgtac tgtcaagaat tacacaggct gaagcactgt ggatcgcggg gggagatcag
     1981 tgggattata tcagctactg gaaggataat gccgtggaag acgcgatcaa ttacctcata
     2041 gaagaaaaaa agatcactgt aggtggtatc agcgccggaa tggccatcat gggcgatgct
     2101 tatttttcag ctcagacagg cactgtaacc tccgatgaag cactgaacaa cccgtacgat
     2161 gaacaggtgc aattggggtg gaatgatttc atcagaaatc ctgccatgac cggcactata
     2221 acggataccc attttgattc tcctgaccgg aaaggaagac tgatcacctt catggcaaga
     2281 ttatttacag actatggttt cctgccggtg ggtattgcct gcgaagaata cacagccgtt
     2341 tgcatagata cagcaggcat agcaacagta tatggcgagt atcctgacta tgaagacctg
     2401 gtgtattttc ttcaggtcaa ctgtcaggaa cccaattcac cacaacaatg cattccgggg
     2461 cagcctcttc agtggcagct ggacgaactc accgtgcatg caacagtgtt ggccggtacc
     2521 ccttcaggta catcttctat tgatctgtat gatatggaga atattaccgg gccggttttt
     2581 gcttttcaga actggcaggt gaacagcgga acactggaag tgttaccatt cgaagaagca
     2641 tacacatgcg attccctgat cagctatgtg aacgaaatga tcctgccggt ctccttgtac
     2701 cccaatcccg cttctgaagc aattgtattt caggctgccc ccggtatcta ccgtattttc
     2761 cgggcagacg gacaggaagt agaacagttt tacagtgacg ggagtgaggt gctcctgcag
     2821 atcagtctgt atccggcagg ttactatgtt atatccggac agggttttgc ctgtcctttt
     2881 atgaaattcg actaatttcg ttttgttatc attgaacgtc cgggtataaa cgcctgtttc
     2941 tatgcgccat caggtgccat acgaaagccg tgaacttatc agccgccctg ctgttcaatt
     3001 tgtaaccaaa taccatgaaa ctacagctct ggaaactcgt actctctgta ttgttgtgca
     3061 tcaccattgg agccattggt gggtttttta caagcggcga gatcgacaca tggtatgcag
     3121 ccctcgagaa accgccactt aatccgcctg actggatctt cgccccggta tggaccaccc
     3181 tgtatattct gatgggcatt tctctggcac tttactggtc tgcagaatat ggcaactcca
     3241 aacgcaccgg gttcatggta tttttgattc aactgggtct caatctgctg tggtcactgt
     3301 tgttttttaa actgcattct ccgcttctgg cgttgataga catcgtttgc ctgatcgggt
     3361 tcatcctgac caacatgtac ttttttgcca gggtgagccg gcaaagtgcc ctgctgcttc
     3421 tgccttatct ggcctgggtt tcttttgcca cttatctgaa tctctctgtt ctgcaattga
     3481 actaagcaga gaggctggat ttactatctt tacctcatgc agcaatttgt ggtttcggca
     3541 cgtaaatacc gcccgctttc gtgggatgag gtggtagggc aggatcacat tgtttccaca
     3601 ctccgcaatg ccatcaaaag tgaccatctg gcgcaaagct ttctgttttg tggcccaaga
     3661 ggtgttggga agaccacctg cgcccgcatc ctggccaaga ccatcaattg tgaaaacctg
     3721 agtccggaag gagatgcctg caatgagtgc gaaagttgtc tgtcatttac gcaaaatgct
     3781 tctttcaata tccatgagat agatgcagca tccaataact cggttgatga tatcaggagc
     3841 ctggttgacc aggttcggta tgcaccccag agcggcaaat acaaggtcta tatcatagat
     3901 gaggtgcaca tgctctctgc ccaggctttc aatgcctttc tgaaaaccct ggaagaacca
     3961 ccttcctatg tgaaattcat cctggctacc acagagaagt ataagatcat ccccaccata
     4021 ttaagccgtt gccagatctt tgacttcaac cggatcacga tcagggatat gatccgccag
     4081 ctgaagaaga tctgcggaaa tgaaggcatt gtggccgatg atgaggcctt tcactttatc
     4141 gcccagaagg ccgatggcgc catgcgtgat gccttgtcca tctttgaccg catcaccacc
     4201 tttgccggaa agaatatcac tgctgcagat gtaatagaga acctcaatat tctggattac
     4261 gattactact tcaaagtggt cgaaatgatc gcagaagaaa atgcagccgg cattctgctg
     4321 ttgttcaatg aggtgactgc aaaaggattt gacggcgata ctttcctgat cgggttggct
     4381 gagcacctgc gaaatctgct ggtatgtatg gacccctcta cggtgtcttt gctggaaacc
     4441 agcgaagctg ttgcggccag atataaagaa caggcagctt ctgcagatgc ttccttcctg
     4501 ctgagtgctc tgaatgtgct caatcaggca gacattcaat accgtgtagc gaagaataaa
     4561 cacttacatg tggagcttgc tctgatgaaa ttatgctaca tcagacatgc tgtcagaaca
     4621 cccggcctgg aatcctcccc tgaaaaaaaa acggctgacc tgaccaataa gcctcctgtt
     4681 gtgcagagaa aagaggttca ggaagaactg aaaacctctc ccaaggaaga agaggcagaa
     4741 aataaaccac cactatccag gcaggaacag gaaaaaaagc ccgaatcaac cggaccggtg
     4801 gtcaccccaa gtatccgcct cggtggtaaa aagaaggaaa aggaaaccac tgagcaacca
     4861 gaggtaagtc cacttgaaga acgcagggaa atttctccgg aactgttctt catggcctgg
     4921 aaacagtatg ctgatctgat cagaaaggat aaggtgaata tggccatgat gatggatcac
     4981 gccagggtaa aactggatgc cggtaatgtg attcagatac agctggattc tcaggtggaa
     5041 gtgaatatgt ttcgcgatgc cacacaagac ctccgccagt ttctggcaga tgccatgagc
     5101 aatcatcagt tcgacctgga attcagtgtg gtcgaggcag agaaggtagt acaaaggcct
     5161 tataccattc ctgaaaaata caagaagatg gaagagatca atcctgccat ccgcgacctt
     5221 aaggaacaac ttggactgga acttgatata tagatactaa catatgaaaa gagtacaaat
     5281 atttatgatg ctgtgcatag tatcatgcat cagcctgatg gcgcaaacac cgccttcctc
     5341 atcttcgtca acctatggtc agcaaaacca gcaggtcaac ccccagaatc cgcccaacaa
     5401 ccagaatcag ccacaggtat cttccggctc aataatagtt ccgggaacag tgggagggta
     5461 cacagatccc ctgcagcttt acagcactcc ttcttcttca agtaaggtca actacgcagc
     5521 caagaatgac ataccggtca tggtctttaa aaatgaccct accggcaccc gcatgtaccg
     5581 cctgcagaac gggctgactg tatatctgtc taagaatacg ctggagcccc gcattcaaac
     5641 ctttattgcg gtacgagcag gttctaaaaa tgacccggca gaaactacag gtttagcgca
     5701 ttacctggag catatgatgt tcaagggaac cgatcatatt ggcacgacca actgggagcg
     5761 tgaacggatc atgctggaaa gtatagcgca actctatgaa caacaccgca acgaaaagaa
     5821 cgaagacaag aagaaagaga tctatgcaga gatcgatcgc ctgtctggag aagcagctaa
     5881 actggccatc ccaaacgagt atgacaagat gatcggttcg ctgggtgcca agggtaccaa
     5941 tgcctggacc agtttggaag aaactgtgta tgtaaatgat attccttcag ctgaactgga
     6001 gcgatggctg atcgtggaaa gtgagagatt ccatcggctg gtgctgcgtc tcttccacac
     6061 agaactggaa gcagtttatg aagagtataa tatggggcag gaccgcgacg gcagcaagat
     6121 gtggaaagag ttctataagc agcttttccc caccaatacc tatggcacac agaccactat
     6181 tggtgaagga gaacacttga agaaccccag tatgtataat atcatcaact actttactac
     6241 ttactatgta cccaataata tggcgatctg tatgtccggc gatctggact acgataagac
     6301 catcgccatg atcaataagt attttggagg ctatgaacca agggtggttc ctgcttacag
     6361 acctaaaaaa gagtctccta ttaccagtgt gaaacgcagt gaagtatggg ggcaggaagc
     6421 tgaatatctg tttatcggtt atcgtctccc aggcagcaat accgatgacc tgaaaaaggc
     6481 caaggtcatt gctgaactcc tctccaatgg cagtgccggc ctgctggatc ttgatctgaa
     6541 ccagcagcaa ctggtgttgg aatcgggcgc atttgttgac gaaatgagag attatgctgt
     6601 gctggttctg tatgcaaatc ccaaagaagg acagtctctc gaagattgcg aacagctcct
     6661 gctcaaagag atggagaaga tccggcaggg tgactatgct gactggctga tggacgcagt
     6721 tattgacaat atgcgcctcc agcagacccg gaattttgaa tcgaacaggg gaagagcagg
     6781 tgcacacgtt cagtctttca ttcttggaaa agactggcaa agcctggttg atgaattgga
     6841 tgtgatggat gctttttcac gttcacagct gactgatttt gccgtgtcca acctgcgtaa
     6901 caacaattac gtggtggtgt ataaaaggca gggcgaagac aataatgtgg tcaaggtaga
     6961 taaaccccag atcactccgg tagaagccaa tcgagaaagt tcttctgact ttctcaggaa
     7021 gttcatggac attccgtcat tggccacaga acctgaattc gttcagtatg acaaggccat
     7081 tcagacctct accctggagt caggcataca gttcgattat atcaacaaca aactgaatga
     7141 cctctttaac ctgtattaca tctttgatat gggaagcgac aacgacctcc tgttgtcact
     7201 ggcggttaac tacctgcctt accttggtac cgacaagtat tcagcagctg aaatacagga
     7261 aatgttcttc cgctacggac tcagtttcaa tgtctctacc agtcgcgatc aaacttatgt
     7321 aagcctctca ggtctggaaa agaacatgga gcagggcatt gccttgtttg aagagatcct
     7381 gaatagtgta aaggctgaca aaggcaagta tgaagaattg gtcaacatca tgctgaaaga
     7441 tatggatgat gccaagtcca ataagaatgc cattctcttt caggcattat tctcttatgg
     7501 acagtatggt gaatcttcac ctttcaagaa tcagctgttt gagaaacaac tgcgcagcgt
     7561 gaaccccaat gacctgacag ataagattca ccagcttgaa tcttatccgc acaagatctt
     7621 ctactacggc tcagcagatc gtgataaaac tgcagcactg atcgatcagt atcatgtgct
     7681 gcctaaaaaa cagagaagca taccgccggc aactgcattc actgaacagc ctacagacaa
     7741 gaaccgtgtg ctgtttgttc agtatgatat ggttcaggcg caaatactcc gcatttcaaa
     7801 agacgaacct tttaatagta agttgtatcc tgccgcagta tcgttcaacg aatactttgg
     7861 aagcggcttg tcttccatag tttttcagga gatcagagaa accagggcat tagcctattc
     7921 tgcatgggcc aactatacca ttccgggtaa ggccaatgaa tctcactatg tgcgcagcta
     7981 tgtagctacc caggcagaca agatgcaact ggccatagaa gcaatgaatg agatcatgaa
     8041 tgagatgccg caggctgaag agcagtttat ccagagcctg gatgccagca gaaagaaaat
     8101 tgaaagtgaa aggatcacac gaacaggaat ttactggagc agggaagctg cagcaaaacg
     8161 cggactgaat tacgacatta gacaggacgc ctacgaagcc ctcagcggta tgagcctcta
     8221 tgacctcaac aggttctttg aagagcatat tgctgacaga acctatacct atctggttat
     8281 tggcgacaaa aacaaactgg acatggacta tctgcgcagt ctgggtgaat tcaaagaact
     8341 gaccctggaa gagatcttcg gatactgaac aatgaattga tcattttaaa gcctcccgga
     8401 aggggggctt ttttgttaat cctgtaacac aaaaccaatt ccgatcagtc ctgctccgat
     8461 cacataactc ggaatgtgca gccatccaac aaactgccca agcatgatgc actctatggt
     8521 tacaaagcca accaggatga acccctgaag caatgcccag taagaatact gcatagcacc
     8581 cttgaatgtg atccaggcaa tgaacaggct cagcaagcca ttagacagca acaggattat
     8641 acccggccac gtgtaattat gaaaaggagc ctctttcagc cattccagag gtaagtgaac
     8701 ggatgctccg gtcggatcca tgatcatcat gacaccgcca acagtggcag ccagaccgtt
     8761 aagcagcaac aggatgaaca atataagctg caatctatac ataacagcgg tttaggttca
     8821 ggtgagttga aattaccaaa ttgctgcaca ttcttcatga acaagcgttg aaaagccttg
     8881 tttattgtgg gcttgtgtgg attctgacag agatatccga tgttggcaaa tcccgcacag
     8941 gtccgtaatt tcgcagttca aaagagaaga aatcagatgg cagaagtgat cagaatgccc
     9001 cgtatgagcg ataccatgac cgaaggtgta tttgtggagt ggcataagaa ggtgggtgat
     9061 aaggtaaaaa gtggcgatgt gctggctgaa gtggaaactg ataaagccac catggaactg
     9121 gaaagctata acaacggtgt gctgctgtac attggagtgg aagccggaaa ggccgccccg
     9181 gttgacagcg tcattgccat tattggtaaa gaaggagagg catttcagga attgctggat
     9241 gcagcacaaa aagaagcccc tgctgccgaa aagtctgcag aagagcctgt aaaggaagaa
     9301 ccaaaaaaag aaactccggc acccaggccg gagcctgtgc aggagaccat cacatttcaa
     9361 gaaaatggcg acggacgggt caaggcatct cctttggcca agaagatggc agcagagaaa
     9421 ggtatcgccc tgacccaggt gagcggctcc ggcgatggtg gccgcatcgt gcgccgtgat
     9481 atcgaaaatt ttgtaccggg cactgaaacc agcgaagttg caccggtcat caatattcct
     9541 gcctatatag gtgaagaatc ttttgaagag gtagctgttt ctcagatgcg aaagaccatc
     9601 gcaggcaggt tatctcaaag taaattcact gctccgcact tctatctgac catcagcgtt
     9661 gatatgtcca atgctatgga tgcacgcaaa cggatgaatg agaacagtcc ggtcaagata
     9721 tccttcaatg acatcattgt aaaagcatca gccctggcat tacgcaggca tccgggagtg
     9781 aacagcagtt ggctgggcga taagatccgc atcaaccacc atgtacatat cggagtggcc
     9841 gtggccatca atgacggact ggtagtaccg gtcattcgtt tcgccgatgg caaatcactg
     9901 tcagctatca gcgccgaaac tggtgatctc gctaaacgtg cccgcgaccg taagctgcag
     9961 ccgcaagact gggaaggaaa tacgttcacc atctccaacc tgggcatgat gggaatagaa
    10021 gaatttaccg ctatcatcaa tccgcctgat gcctgtatcc tggcagtggg cggtatcagt
    10081 gaagtaccgg tagtgaagaa cggacagatc gttccgggtc atgtcatgaa gatgaccatg
    10141 agctgcgacc atcgggtggt agatggtgcc accggtgcgg cattcctcca gacactgaaa
    10201 ggattcctgg agaatccggt cagcatgctg gtctgacccc tcagggaagg atcacatcat
    10261 gtatgcacca gatgggcagg tgaaccccgc cttttaacag cacgtagtgt tcagtagtgg
    10321 cccagatggt ggtttctacc tggcaaagtc cttctatggt ctcaaaaatg atcctgacct
    10381 tagagtgata agtattgccc agtatcatag cctgaaacag caattgcctg cgtttatcat
    10441 gaaactcagg gtttggaggt aagacctcgg aacgtgaaaa gtgaagtgca ggaatagctt
    10501 ctttttctac atgttgcagg gtgtggtggt ccataggttg tctgtttgga acaagaatgt
    10561 attcaggtat gttttgcaaa ttagagcagt acaaaatgaa tagcaatatg tcagacatat
    10621 ttgcaggaaa aaccctggtg ttgggggcgt caccaaaacc tcatcggtat gccaacaagg
    10681 ccatcaggaa actgagagaa tacgggcatg aagtggtggc tgttgctaaa agaaaagaca
    10741 ctgcctgggg ggtggagata gtcacaggca tgcctgatat gaaagatatt gacaccgtga
    10801 ccatgtacct ggggcaggaa aatcagaagg aatactattc ctatatcata gcgttgcatc
    10861 caaatcggat cattttcaat ccgggtgcag agaatcctga gcttgctgca atggctaccg
    10921 aagccggtat cgaagttatt gatgcctgta ccttagtttt gctggcaacc ggccaatact
    10981 gatttgtcca gaccatcgca taccgtagag aactacctca aatgcatctt ctccctgggg
    11041 cagggaaagg atgagcgtat tcccaccaat gccatcgcag agaaactggg aactacggct
    11101 gcttcagtaa ctgacatgat caaaaagctt tctgccaatg gttatgtgca ctatgaaaga
    11161 taccacggtg tcacactgac cagaactggc agacagatcg ctgtggccct gatccgaaga
    11221 catcgtctgt gggaaacttt cctggttgac aaactgggtt tccagtggga tgaagtacat
    11281 gatcttgccg aagaactgga gcatatcaac aacgatctcc tgtctgaaag actggatgct
    11341 tttctgggta atccaacggt cgatccgcat ggcgatccca ttccggacaa ggacggcaac
    11401 attcaatacc atgaggatgt gtcactggat caactggagg ccgggcaaca gggcatcatt
    11461 gtcggggtaa ccgaccacag caccgatttc ctgcaatacc tcgaaaaaaa tggcctggtt
    11521 atcagggcag tcgtaaaagt catgcaggta gaagcatatg atcagtccaa gcgtcttgaa
    11581 ctggacaagg gaaaacaatt gaatgtctca cacaaggttg ctgccaatct gcttgtcagg
    11641 aaaatgcgct gatctgcata cgtttgcctc gtgacaccgc cagtgccgcg atgaacagca
    11701 cacccagtac aacgaccata ctgccggaga tcgaagcgtt gagccatagt gccaggtagt
    11761 atcccagaat ggatgaagtg atcccgatga gtgtggccag cagcaacatg gtcttcagct
    11821 tgtgtgtcag gagataagct gttgccgccg gaatgatcag gaaggctacc accagaatgg
    11881 cacccacact ttcgaaggta aaaaccgtcg taacagagac actgctcatg agcaaatagt
    11941 gccatccggc aactgaaata ccaagggtgg tggcatactc cggatcgaag gtggtgatga
    12001 acaatccctt atatccaaga ccgatcatcg ccagaataaa gatcagtaaa acgcccaggg
    12061 tccacacggc aaccggaatt cccagccagg tctcaaatcc cacgaaagcg atctctccat
    12121 atagaacgca ttcctgatcc agatctacct tgtcagcaaa gaaggtgatc agaatgaccc
    12181 cgatggcaaa taaccaggtg aaggagatgc caatggctgc atcttcctgt actcttgccc
    12241 gcttgctgaa cacttctatc aataccgtgc agaacagacc gaacgcagcc gcccccagta
    12301 acatgggaac actactgcga cttccgctga tcagaaatgc gatggcaatt ccaggcagca
    12361 cggcatgcga tatggcatca cctaccagtg ccattttgcg cagcaccaga aagcatccca
    12421 gtaaaccgct ggtggcggct accaatgatc cggtcagtat gatccagaag gcatcactca
    12481 ttaccatggg atctctttat tgtgtggatc cttttccgga tagtccagca acgctgccag
    12541 tctttcttcc agttcaggtg taatgatgtg ttcgatagct tcggcatctt catgtacatg
    12601 atcgctggca atattcattt tctctgtcag gtacatttcc cataacctgt ggatacgaac
    12661 tatgcgcttg ccttcggtca tgccttccgg tgtaacagca tatttgttct gtgattgttc
    12721 tatcatccca ttgcgctgca actgtcttag ctgacctgcc agagcatcag agaggtctgg
    12781 tcttttctcc atgatctcag gcaggccgaa ccattgcttg ccgggggtgg cttcttccag
    12841 gtgatacaaa gctttgagca aattctcccg ctgcactttg ttgcggtatt gccttttttt
    12901 ccaccacctg gagaacacgc cttttcccgg tgcgaacagg atgctggcaa ccacgagcag
    12961 cgaggtcatg gtaactaccc aggggccggt gggcattttg ttgttggcaa atgaaacata
    13021 tgcacccaga attccacctg ctacaccaaa caatactgac aacaatatca tgacccccag
    13081 tttgttggtc cagaatcggg ctgctgcagg tggggtgatc agcatggctg ccatcagtac
    13141 cacacccact gcctgaatgc ctgccgctac tgcaagtacg gtcagcgtgg tgagcagggt
    13201 ctcatagaat cgaacaggca tgccggttgc tttggcaaaa tccgggtcga atgagagtag
    13261 gcggaatggt tgaaagaaca cagcaactgt gcccacgatg acgatggata ccagactcat
    13321 ggtgcgaatg tcttccggtg ccatggcagc agctttcccc agcagaaaat tgttgagtcc
    13381 gctctgcccg ggtaatcctg tctgctggat ataggtgagc agcacaatgc cgatggcaaa
    13441 gaaaaaactc agcgcaatgc caatggctgc gtctggtttt attcggctct tactggtgat
    13501 cagatccacg aacaggatag acaaccatcc ggttacaaac gctccgccaa gaagaaccca
    13561 gaggtttctt gtctgaaaga tcaggaaggc cagacatact cctggtaaaa cggagtgcgc
    13621 cactgcatct ccggtaagag atcttttgcg cagaaaggca aaggtgccga tagtggaaga
    13681 ggcaataccc agcaacacca tgcctgccag cacgaaccgc acattgggat cctgaaggga
    13741 gaagaagcgt atgaatgatt cagcgttcag gatctgttat ttttctcttg ttggggcttc
    13801 catttcagcc agcatctcac ccacactggt aagcatgttc agtttgccac cgtaggtctt
    13861 gcgcaggtaa tccggaacaa agatgtctga cctcgggccg ctggctacca gttgtgtgtt
    13921 cagcaggatg atccagtcaa aatactgata ggcactctgc agatcatggt gcaccactaa
    13981 tatggtcttt ccttcatcac gcaacttgcc gagcagctcg aaaatggtct gctctgtagt
    14041 ggcgtcaata ccggcgaaag gttcgtccat cagatagatc tctgcctgct gtgccagggc
    14101 acgtgccaga aatactcttt gctgctgtcc gcctgataat tgtgagatct gccggtattt
    14161 gaaaccttcc atcccgacct gctccagaca ccgtaaggcc agtgcttcat cagccttcga
    14221 taaacgactg aacaatcgcc gctttttgaa tcgtcccatc agtaccacgt ccagtacgct
    14281 ggcaggaaaa tcccagtcaa cggttccacg ttgtggcaca taggccacct tcccccgcac
    14341 ttcatcgatc ggacggttga acaaacgcac gtatccgctg tcgcctgata ccagtcccat
    14401 catagctttg atcaaagtac tttttcctgc gccattcgga ccaatgatac ctgcaagcac
    14461 acctgaaggc aatgaaaaat ccacacccca aagggcaggc ttgcgttgat agctcacagt
    14521 gaggtcatgc acctcgatca caggatcggc aactttcgtg gtcatggttt acgggttaat
    14581 gcattcacta aggtttgtac gttggatctg accattcctg tataggatcc ttcgggggta
    14641 tctgcagcac ccatggcatc actgtagagt gtgccaccca gttgaatggg atgccccttt
    14701 gcagcacacc cgtccatgac agaacgaatg gatttttcag atacgctgct ttctacaaac
    14761 acagcaggaa tgccttcggc aatgatcagg tcaaccatat cggtcacatc tttcagcccg
    14821 aattctgcag ccgtagaaat accctgcaat cctactacct tgaactgata ggctcttcca
    14881 aaataccgga aggcatcatg agaggtgatg atggtgcgct tgtttgccgg aacggcctga
    14941 agtgtctgac tgatctcgcg gtgcaatgcc agcagggtat cagtataggc agctgttcga
    15001 agtatgagcg agtcgctgtg ttccggcata ttggatgaaa ggaaatatcc cagtttctgc
    15061 acagcacctg cccagatgcc tacatcaaac cagatatgcg ggtcataatt tttttgctga
    15121 gggccattcg catccagcag gttctgcggc ggaatgcctg cacccatggc atataccggc
    15181 ttggtttcgg caagctgatc gaagatctcc tgcatctttc cttccaggtg caaaccatta
    15241 tatatgataa tatctgcctt gcgcagcaat accaggtcgc cctgggtggc cttgtacaag
    15301 tgaggatcca ctccgggtcc catcagagcc tctacccgtg catccgggcc cgcaatatgc
    15361 ctggtggcat cggccaccat accggtggtc actacaatga gagggccatc agtattttct
    15421 gtttcgggtt gtttgcatgc ctgcaacagg atggcacaca ctaacgcagt gaaaatggag
    15481 gtacgcatga agcaaaatta cattttgtat ttagaatgcc aaaaataaaa gattagatag
    15541 ctctaaattg cagcttgcgc tgcttcctta cctttgcgct gtgaaaaatc cctttgtaag
    15601 aggagatatc aggcagtaca gtaagatcgt ggaagacacg gagatcgctg aattcccctc
    15661 aggaacggtg catccggttt actctacctt cagtctggcc cgtgatgctg aatggagcac
    15721 ccgattgttt gtgctggaga tgaaagaagc tgatgaagaa ggaattggca ctttcgtaga
    15781 ggtgcgtcac cattcgccgg cattggtggg catggaggtg atcttcacag ctaaacttga
    15841 atcagtgctt ggtcacgagg tgatctgctc ttttgaggcc catgtagaag agcgccttat
    15901 tgccacaggc cgcactgggc agaaaatatt gaagaaagaa aaggtggagc gattgtttgg
    15961 ctccttaggc acagaagtat gaaaccccag caactgaata tcagaaacaa gcgggccgga
    16021 tttgattttg aactcattga ccggtttact gccggtttgg ctttacgtgg aagtgagatc
    16081 aaatctgtaa gggaagggaa ggccagtatc aatgaagctt actgtctgtt caaagacgat
    16141 gagctgtggg tcaggaatat gcatatcgct gaatatgata tggccaccaa ctggcagcat
    16201 gagccactgc gcatgcgcaa attgctgctg gaaaaaagag aattacgcaa acttttatca
    16261 aaggtgaaag aacgaggtta taccatcatt ccactgaggg tgttcatgtc tgaccggggc
    16321 tttgccaaaa tggaaattgc tctggctcgt ggtaagaaga gccacgacaa acggcatagc
    16381 atcaaagcca aagacgaaaa aagggacctt gacaggtccc tctccaaatt tcgttgatca
    16441 gtttacttaa ttcctgccgt tgtcatacac ccagttgatg gtaaaattca ctgaggtaga
    16501 agtagaaatg gtcactgtgt cttcattctg ctggtagtta tcagaaaaat tcacggtcca
    16561 ggtcatgtcg ctccatgcga ggcgtggtat atcatatgac aaataaggtt ccagtaaccc
    16621 tgtcaggtcc atgtttacag tagtcttgtt ctgagcatcg tttgaccaga accagtagga
    16681 gttcttagtg ctggtttgca gtacatcgtt ggcgtttgcc gggtctgtac gggtttcggt
    16741 tgatgaaacc tcaccattgt ccttcacctt gatggcgtaa gcataaccat agctttcagt
    16801 tgcaccatca gtattaataa agatgttggt gccatcgaag tcatagtagg ttgttatggt
    16861 ctgtccgtca atggtgttca ccactgtacc ctgcatactt tccatcatcc agttctgagc
    16921 caggcgggca tcacgtgaat gaaacgaaaa gaaagggtcg tcttcacctt ttttgaaaca
    16981 actgctaaag agcagggtca tgcctatgat cgtggtactt acaaggatct ggcgggtctt
    17041 tttcatactg gtcgctgatt gaatgcgcaa agttaacact attcggaatg ttagagggtt
    17101 taggaaagat tttcgttgca agccgctcag aataccttcc cgggaagcat aggtacaaac
    17161 cggaacagct caccgtcttc cctgaccact gatccgtctg cctgacgagt aagaatgacc
    17221 atgacctgat cttcttcctg ccccaggggt atcaccagtc ggccgccggg tttcatctgt
    17281 tctaccagag ctggtggaat ttccggggca gcagcggtta caatgacccg gtcaaacgga
    17341 gcgaaaacgg gccatccggc atagccgtcg ccaaaagtgg tcttgatctg cctgtacccc
    17401 agactgtcca gcatttttcg tgatttctca tacaacggcc ggtgcctttc aatactgaat
    17461 accttgagcc ccatctctgc cagcacagcc gcctgatacc ccgaaccagt gcctatttcc
    17521 agcactttca tgcccggagt agcctgcaga gcctggctct gataggctac cgtatagggt
    17581 tggctgatgg tctgacctga cccgatctgg aaagctttgt cttcataggc atgctcaata
    17641 aaggctttat caaaaaaaac gtgacgtgga acctgctcaa tggcatgcag cacccgctca
    17701 tcagaaatgc ccttgctgcg cagtaattcc accaactgac tgcgcatgcc tttgtgcctg
    17761 tatgtatcta ttgccgacat ctgtatgaca aacctctgac cggaagaaaa tttcatgtcc
    17821 ggtcaaaatt ttacctttag ggcacaaaaa ttgtaactac cagccggatt ctgagatcaa
    17881 attgtaaaca cccttcaaaa tttctgacca ccaatgaaaa aaatgcttct ggccacactg
    17941 gctctcctct ttgttaccgg aacctcggta tttgcacagc agaatatcgg actgaatgct
    18001 gaagccgatt accctatttc accagctgac tatggcctca catttgaaga gatccatgta
    18061 gaaacacccg ataagaaaga ggacaatacc aacctcatgc tttttggctg gcacctgatt
    18121 cccaatgata agacttcacg caaggctatg atcattgccc acaacggcca gggcaacatg
    18181 tcttatgccc tggaaaatgc cggtatcttc ctcggtatgg gataccacgt gttcatgttt
    18241 gattaccgtg gctacgggca aagcgatgcc ttcaatgtaa gccctaaatt ctatatctat
    18301 gctcagtttg ctactgacct gaatgccatg gttgactatg tcaagaaata ccatacggtc
    18361 atgacctgca gcatctatgg ttatggcata ggcggcggac ttgcccttgg cgtaggagcc
    18421 agcaatatca agacttacag tgtcatcgca gatgcaccat acatcacgct ggaatctatc
    18481 gaaaagacct acaaggaaat gaccggtcag aagatcttca tgcctctggc atatgacaag
    18541 gccatgatcg aaccgggtaa tgcactgcct gtgaaaggcg atcacctgcg tggtatcatg
    18601 ctcatatgtg gtgcaaacgg cgatctgatc aaagctgccg atgttgcggc acttgccaac
    18661 taccacaaaa aggtcactac cgtgtacacc gttcctggcg tgaacaacga tatgaatttc
    18721 gaatccaaca aagacgagta ctacaagcag gtcaagtcat tcctggacgg tctcgactga
    18781 tattgttaag tgcatttcaa tgatacatga aggcctcctg tactgctgtg caggaggcct
    18841 ctttttttgt aacattgtgg caaatcttgc agatggtaca gatcaaattt ggaacagacg
    18901 gctggagggc catcatcgcc cgcgaattca cgaatgacaa tgtggcccgg gtggcagcag
    18961 ctactgcaga atgggcaaaa gcaaggtcag aaggtccggc tgtggtaata ggtcatgatt
    19021 gccgttttgc cggtgagatg ttcgcagaag taacggccaa agtgttgtgt gctgccggag
    19081 taaaagtgta tatagcaaaa ggttatgtca gcacgccaat ggtcagcctc ggcgctaata
    19141 aactaggtgc atttgccgga gtggtcatta ctgccagcca caacccgcct gcctacaatg
    19201 gtttcaaact caaaagcgga tacggcggac caaccattcc cgaagagatc tcctgggtgg
    19261 aatcacacat cccggaagta acagaaatac cagaaacaga tctggatacc tgggcagctc
    19321 agggattgct ggaatgggtt gacctggaag aaatgtacta ccagcatgta ctggcctcct
    19381 ttgatatgga tctcatcaac aactcaggca tacgcctggg ctatgatgcc atgtatggcg
    19441 ccggtcagcg catcgcatta cgcctgctca gcaatatcgt tccgctgcat gtggatgata
    19501 atcccggatt ccacgggcag gctcctgaac ccatccaccg caacctgacc gaactcagtg
    19561 aactcataaa aggtgacaac agtatccagg caggtcttgc caatgacgga gatgcagacc
    19621 gcatcggcat gtatgatgaa cagggaaatt ttgtggacag tcaccatatc atcctgctgc
    19681 tgatacacta cctccatcat tacaaaggtt acacgggcaa agtggtcatt gccttcagtg
    19741 tcagcgataa ggtgaaacgc ctctgcgaac actacggtct ggagtacgag gtgacgaaga
    19801 tcggattcaa atacatctgc ggaaaaatgg ttacagatga tgtactgctg ggtggtgaag
    19861 aaagcggtgg tatcgccgtg aaaggtcatg tgcctgaaag ggatgggatc tggtgcggcc
    19921 tccttctcat ggaattcatg gcacaaacag gaaagaacct caaccagctg atcgaagaag
    19981 tgtatgccat cacaggttcc ttctgttttg acaggaatga cctccacctg actgatgcgc
    20041 tcaagaacag catcatggaa aaatgtgcag ccggtgcgta ccagtcattc ggacaatacc
    20101 aggtacagcg ggtagaagat attgacggat acaaattcca cctggatgat cacagctggg
    20161 taatgatccg ggcttccggc acagaaccat tgcttcgtgt gtatgccgaa gcgtctgacc
    20221 gtgccgctac agatgccatc ctggaagcag cgaagaaaac cctgcttggc taattactgc
    20281 gaaggccccg atccaatgca gatttgcacg tacaaacaga gaagtctttc ttttcgcagg
    20341 gcctttgcgc atgtgcggtc aatcctgttt ttatttctac ttttctcttt tcattcaacc
    20401 atcactgcac aaacagagaa cccacctccg cttcgtgatt ttctgaccac ttcagaagaa
    20461 ttcaataaag gtcgtctggg tctcgtgctg ggtacagagg gagtgttgta cacaggagct
    20521 gttcttgcat tgtaccagta ctggtataaa gattatccaa ccgaaccatt ccactttttc
    20581 aatgacaatg ctgagtggct gcaacaggac aaagcgggtc atttctatac ggcgttcttt
    20641 gagaccaacc tcacaaccgg tctctacaga tggaccggaa tggaagacag gaaagcatac
    20701 tgggccggat ttgctactgc gtctgcgttg cagctgacca ttgagatgtt tgatggcttc
    20761 tctgaaaaat ggggtttctc tcccggcgat tttgcagcga atactgctgg agcggcgctg
    20821 gctaccgggc aatatttttt atgggatgaa caacgcatcc gggtcaagtt ctcttctcat
    20881 tttgtcaact acagtggcta tgaccaggca gtgcaggatc gggcagttgc cttgtacgga
    20941 accagcggac ccgagcgtgt gctgaaggat tataacgggc tcaacctctg gctatctgtc
    21001 aatcccacac attttttatc tgaagatacc tggtggcctc actggctgat gatcagtgca
    21061 ggatatggag ccgatggatt gttcggtggc tttgaaaatg cctggtgcga tgatcccctc
    21121 gtgaaacctg aagaatgcga tcctgctcat ttgaccagtt atccgcagat accacgatac
    21181 cgccaatact acctctcact ggatgttgac ctgaccgcaa tagagaccaa cagccccttc
    21241 tgggatatga tgttcgagat cctgagcatc gtcaagatcc ctgctcctgc cattgagttc
    21301 aacggcgatg gcagggtgaa tttctatccg ctttacttct gatcacgaac tgctacctgt
    21361 acaaagtggt caaagaagaa ctcatgcttc agtcgaagag atttcactaa gatcattcca
    21421 cgatcagaca gaaaatagcc atactctttc aggtcgaatt gatgtggatg gagaatatct
    21481 ccgggtatca gcctgaacag gtgtttgaag aatgagtggt tgtgcgcatc aatgctcact
    21541 accagcttgc cccccggctt tacccagctg accagcctgt cgtagctgag ccgaatatca
    21601 ctcacatggt tgatggcatt catgcagaac acgatatcat actgcctgtc gggctcatac
    21661 atttccagcg gactgtgaaa gaaccgcaca ttgggatgat ctgccttgtt gaaatgatcc
    21721 agttcatcgg catactgatc cagtaaagga tccatggcgt caacagaaca gttggacagc
    21781 gccatgaaga tgcctgcagg accacaaccg gcatccagca cttccatccc atccgccacc
    21841 tcacaggcat cagagatctt cgccagcaga tcctgccagt atcctcgctt ccagtcatgg
    21901 taagcaccca cttccttgtt cttcagatag cgctgccacc aggcaagttc cgccttctgg
    21961 gccaggttcc acttcagcgt tttcttcata tttattctaa gaaatatgaa gtgaagttac
    22021 cttgtaaatt gaaaattaat gtcaggtgaa tttcgttatt ctaatgaaat tgtttttttg
    22081 ttctggcata gaggttgtct gtgttaacat ataacacgca aatgttgtca caattaactt
    22141 caatatatgt gtttacagtc ttcaaaaggc gtttggtttg tcttcccatg atgttattcc
    22201 agtgcaccac aaggatctcc ttacatgaag aatcaacaaa cccagttgaa gggtttagcg
    22261 gtttgtaaaa ttgcacataa gtctgaaagg aatcaaggcc atataaggtg tcaggtaaat
    22321 aaagaggatt cattaagagt ggatggtgat caaaataacc atatctgttc catttcagat
    22381 tggggaaccc gccagcatcg cagcttcgca tgaagaactg atttgatcct gtctggtcaa
    22441 atcccattac taataaagga ttggtaagaa aattttcttc gaaataattc ttacccgaaa
    22501 cctttgtgta ctcgtcaaca aaggccagat atttaacctc atcaagaata tagaggttat
    22561 cggaatcgat atgatatatc cgggcgtact cttcaatttc agattgagaa aggtactttg
    22621 acgaatgcat tcctattgtt gcccgcagga ttggttggca ggatgactca aagaccagta
    22681 atgtagttgc caaaaaaagg gaaataaata gttcaaattt tggcaaccac atgtttatgt
    22741 caataaactt caatatcaaa tgtaactttc ccatttggtg agttattgta atttactgca
    22801 tatatacctt gttttactac cagggaatca tatccaaatt catttgcgat ttcagaaggc
    22861 catgtccaat cttgcaatat ttgtgtatat ccatttccat tatcaggagt agtactgcaa
    22921 ttcatcagca atttagtatc tcc
//
LOCUS       DAOMAX010000010        22203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_239_length_22203_cov_4.167735, whole genome shotgun sequence.
ACCESSION   DAOMAX010000010 DAOMAX010000000
VERSION     DAOMAX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22203
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239_length_22203_cov_4.167735"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..273
                     /gene="rpmB"
                     /locus_tag="PK742_01390"
     CDS             40..273
                     /gene="rpmB"
                     /locus_tag="PK742_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQU75270.1"
                     /translation="MARVCDLTGKKTISGNNVSHSNRKTRRKFYPNLQTKRFFLPEEG
                     RWVELKVAASTMRTINKKGITAYLSELRKEGKI"
     gene            289..477
                     /gene="rpmG"
                     /locus_tag="PK742_01395"
     CDS             289..477
                     /gene="rpmG"
                     /locus_tag="PK742_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQU75271.1"
                     /translation="MAKKAKGLRIQVILECTEQKNTDVPGISRYITTKNRRNTPDRIE
                     MKKYNPYLKKMTVHKEIK"
     gene            480..653
                     /locus_tag="PK742_01400"
     CDS             480..653
                     /locus_tag="PK742_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HQU75272.1"
                     /translation="MGKVSKNARVTKGKVSGGGKEMVKVIVTEKSPKTGAYIFKERIV
                     HKDKVQDVLKGNA"
     gene            725..1684
                     /gene="ftsY"
                     /locus_tag="PK742_01405"
     CDS             725..1684
                     /gene="ftsY"
                     /locus_tag="PK742_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQU75273.1"
                     /translation="MALFGLFGKEKKEDLDKGLEKTREGLFSRISRAVAGKATVDAEF
                     LDELEEILISSDVGLDTTVKIIGRLEERVARDKYLNTAELQHILREEIVRLLTDSNTP
                     DYDDFQLPASADPYIILVVGVNGVGKTTTIGKLASQFKSQGKKVLLGAADTFRAAAID
                     QLSVWADRTGVEIIRHQMGADPAAVAFDTVNVAKNKGADVVLIDTAGRLHNKVNLMNE
                     LSKIKRVIQKVYPDAPQEVLLVLDASTGQNALEQARHFTAATEVTAIALTKLDGTAKG
                     GVVLGIADQFRIPVKYIGVGEQVHQLQVFNKKAFVDALFHEPA"
     gene            1681..2997
                     /gene="rimO"
                     /locus_tag="PK742_01410"
     CDS             1681..2997
                     /gene="rimO"
                     /locus_tag="PK742_01410"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQU75274.1"
                     /translation="MKVRSHKKDKVNIVTLGCSKNLVDSEVMLTQLRANEIDAHHQLE
                     DDNSNVIIVNTCGFIDNAKQESIDTILQYASVKSEGGIDKLYVTGCLSQRYRDELMQE
                     IPEVDAWFGTMELPALLEKFQADYRQELLGERIITTAPHTAYLKISEGCNRTCSFCAI
                     PLMRGGHRSRPMEDLVTEASRLVANGVVEIMLIAQELTYYGLDLYKERMLPELLRQLS
                     DIEGLRWIRLHYAYPSKFPLEVIDVMRERNNICNYLDMPLQHASDSVLHAMRRQITRT
                     ETEALVHEIRRRNPEITLRTTMLVGFPGETEEDFEDLLDFVRKMRFNRLGVFTYSHEE
                     GTSGYELEDDVPDDVKQERAAKLMSVQEEIAAEWNQTFVGKELTVLIDRLEGEHFVGR
                     TEGDSPEVDNEVLISVHNQYLRLGDFVQVHITGADAFDLYAEPVQK"
     gene            2994..4598
                     /gene="bshC"
                     /locus_tag="PK742_01415"
     CDS             2994..4598
                     /gene="bshC"
                     /locus_tag="PK742_01415"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HQU75275.1"
                     /translation="MTFTHSKIKLADTGFFPQIMLDYMAGAEALRPFYQHTPGLDAFG
                     EAMSAKAREGTDRSTLVKVLQEQLRNLTLTDAVKDNIQSLADDNTFCVVTAHQLNLAT
                     GPLYVIYKALSAISLCEQLEKQYPGKKLVPVFWLGSEDHDLDEVNHFHAFHKTYTWQT
                     EQSGAVGRMLATGMDALWDSLAEDAGNSSEADAWVNMLRDCYRADRTMSVATTDLLNR
                     IFGTYGLVILDGDHPAMKKACADIILDELQSGNSAGIVQLASEKLSRHYSVQAYPREI
                     NLFFLDNNSRDRITRVADGYELANSRRTFGAEELEELINNQPQVFSPNVILRPLFQQR
                     VLPAVAYVGGAGELSYWMQLKTLFGHHGIEMPVLVLRDSVTLVDHTSCKKLQKLGLEP
                     DAMFGDIEQLVKAYVSQQAGEEIDLEIEKKQLEKTYRSISEKAAKVDPTLEKAVAAEE
                     ASALKALEKLHQRLLKAEKQRFDVAINQLRSVHARMFPNGGLQERYDNLLPWLLRFGP
                     SFPDQLLKVMDAPSDHLHILCEEPQA"
     gene            4671..8246
                     /locus_tag="PK742_01420"
     CDS             4671..8246
                     /locus_tag="PK742_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQU75276.1"
                     /translation="MYRRMESPFEHTRITERYRGLEHLSVPVALVDASFNVIHYNPAY
                     RKLLPQGANKQKLLHLDRMFNGFQDKLKVLQDAGLYAGNIMQFRMPGVQLSETNRIFD
                     LHIARLEQSADLLDGYTINLIEVTEREQELKRLRELSDHHLKFIHHTTSGVLIHCNGK
                     LVYVNPQGVRMVRAADAESLIGRSVVDFVTPEFRTIVVERIRRMNEENLPVEVIEEHI
                     IREDKTTFPAEIFAYPVTYDGAPAIKVIINDITKRKEAQLRLQESKQEYQNLIDQLQD
                     VIFRADEKGKIEFLNSAWTELTGYRVEECLGHSMSDYFVKNSDTDSSFVHVIRQLNNG
                     IGDFENDLLLETATGQHIYVRAKFQTIRDHAGLLTGFSGLLSNIHSRKTILEEQRRIE
                     NALRRNQNLYLSLAKSMQAGSRGLQEACREIARTSCVAMALDRVNIWQFNETGNELTC
                     LTNYIKQEQTFADEGLVLSDFSAYTEIIREERVLTIDDALQDPRVDVFRDSYIIPDGI
                     LSMMDAGIYIGEKLWGVICFDVTESRRDWMPEDRSFAANLADLLSGIIASNEQEETRS
                     ALKKTVELNSTILENAGESIFLISQEGLVEDANNMTSRLTGYSLDELTGSSISRFIPE
                     EFISTGDSVVRNIEGTGTFHRTRTLVCADGRTKTVEISVTRLEDSRMVAIMRDITERI
                     EREKALRESEARLDMALKGADLGTWDFFIKENRIIHNRRWGEMLGYHFEITSVNEQFW
                     EKFVHPADIESSYRKFQDHINGLTPFYEANLRMLASNGEWRWILDKGRVVEWDENGEP
                     VRASGIHQDITALKSIEKEIEDQRIFLQQIINAIPDPIYVRNASDEFVVINNAFAEYL
                     GVDRQMVMQYKFNRNHQFHETFQQLIDPDAEILLNQQPLELKEHKLLNQATGKHTWVS
                     TMKVPLTDADGQVLEVLSISTDISDLKNRETSLKEKVEDLSEKLRNRTGKLEQLTKEI
                     DTFHYSVSHDLRTPLRTIDIFAYFLEKRYSDKLDEEGKSNITQIRNSITKMGTLIDNL
                     LIYIRMGKVPVRKKMVQVSDLVQQVIDEMGRSTDLEQVSFEIGGLPVMETDPDMLRQA
                     LRNLISNAVKFSATARQPVVQFSSSKVEGYSIIRITDNGVGFNMEFKDKLFKAFKRLH
                     SEEHFEGTGVGLAIVERIVQRLGGEIWAESVEQEYTTFFIKLPLH"
     gene            8290..8373
                     /locus_tag="PK742_01425"
     tRNA            8290..8373
                     /locus_tag="PK742_01425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8324..8326,aa:Leu,seq:cag)
     gene            complement(8424..10937)
                     /locus_tag="PK742_01430"
     CDS             complement(8424..10937)
                     /locus_tag="PK742_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU75277.1"
                     /translation="MSRQLTLSTVLIAVLASGIVLYQQISPTQASQTAKDPETDTWQM
                     LVTQQIMASEYQFRKGTENPFEAFNRRSDSRFYLTESGFKAVKRSDADVRLEVTLDQV
                     VADNLPLASTGINWQTESAELLVMDAGTYQIEYMNAPEGFRQNFIIEDPGEPAARVAV
                     KLHMSGSGESRMTNNSYELVAGGQTLFSYGNLRAYDAYGRSLESHMELNGQELALVTD
                     TRNAVFPVTIDPLTTTPDWSYEGNQVGANMAFAVSTAGDVNNDGYDDVVVGAAAYDAG
                     ETDEGKAFLFLGGPSGLSTTPVWEDEGDNESGLMGKSVAAAGDVNGDGYDDLLIGLGL
                     YSGGESEEGRAHLYYGTSTGVTTEPVWWFEPNQAGVGLGFPLEGLGDVNNDGYDDVII
                     GGNGWDGDQVDEGRAYVFYGSADGLADEPDWTVDGNNTDAALGHAVSAAGDVNGDGYD
                     DAIIGGWNYSGAIANCGKAWVFLGGPTGLADSPVWELEGDQSNGYFAFWVSGVGDVDN
                     DGFDDIMIGAKRYDAEFVDEGKAWLFMGSPAGPETTASWTAEGDNDGAEYAIQFGPAG
                     DVNNDGYADVIMGSHFHTEDFNKEGKAYLYLGGPSGLSATAAWTYLGGVNQANFGWTC
                     DGAGDVNHDGFDDVIIGAFKYSNPETYEGSAFVFHGLNSCPTPSSYAVGSLAPTTADV
                     SWTGADDATIYKVTVKGAGESHLYLSLNDSYTLTGLTPSKKYKSWVQTKCGTTWTLRS
                     NVITFTTPPMRTSGEDQGEVYPNPTAGWLHITNSSSDAGIYQVRLFDLNGKMVMEMET
                     GWLEAGIPASVDISHLSAGTYTWQTSCQGKTRQGLITKK"
     gene            complement(10986..12188)
                     /gene="hutI"
                     /locus_tag="PK742_01435"
     CDS             complement(10986..12188)
                     /gene="hutI"
                     /locus_tag="PK742_01435"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQU75278.1"
                     /translation="MLGPVWPDGTDRLAGKDMNLFPTLSNAWLLSENGRIAAYGTMDQ
                     ELPEADEVIDATGRWVLPAFCDSHTHLVFARSREEEFEMRIRGRSYEEIAAAGGGILN
                     SARKLQQASADELEASALERLAEIERMGTGAVEIKSGYGLTVDAELKILRVIQKLRSQ
                     SRLIIRSTFLGAHALPLEYRQDRNGYIKLITDVMLPQIADEGLADYCDVFCENGFFTP
                     EETDTILQAAWKYDIAPRIHANQLAVSGGVQAGVRNNAISVDHLEHITRNEIDCLLDS
                     NTLPTALPSCSFFLNLPYAPGRDIIDAGLPLVLATDYNPGSTPSGKIPFVLSLACIKM
                     RLTPEEAINAVTVNGAAAMQISDRAGSITAGKDARLIITRPMEHLAYLPYAFGGDHIE
                     RTILPTIS"
     gene            12208..13359
                     /gene="aroC"
                     /locus_tag="PK742_01440"
     CDS             12208..13359
                     /gene="aroC"
                     /locus_tag="PK742_01440"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQU75279.1"
                     /translation="MRWEGIFKGTDARLQIKRNLYLRRMPGNSFGTYFRITTFGESHG
                     PAIGVVIDGCPPGIALTEAQIDEALARRRPGQSRITTQRKEPDTCEFLSGVMEGKSTG
                     TPLAILIRNSDQRSKDYDHLADTYRPSHADFTYQEKYGIRDHRGGGRSSARETAARVA
                     AGAVAMAVLKTEGIHVQAYVSAVGDIVLDASYQDLDLSRTDENIIRCPDQETAERMIS
                     LVNAVRKEGDTIGGVITCVASGVPSGLGEPVFDKLHADLGKAMLSINAAKGFEIGSGF
                     HGAGMRGSTHNDIFENRDGKIVTRTNHSGGIQGGISNGMDIYFRTAFKPVATILRPQE
                     TVNSAGQKVSVEGKGRHDPCVLPRAVPIVEAMTAMVLADHLLLQRINRK"
     gene            13361..14734
                     /locus_tag="PK742_01445"
     CDS             13361..14734
                     /locus_tag="PK742_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQU75280.1"
                     /translation="MRKIALHWKILFGLLLGVIWALLSAGLGWRQFTLDWIKPWGTIF
                     INVLKLIAVPLVLFSIIKGITDLSDISKIRRVGLKTIGIYVGTTVIAITVGLVAANLI
                     QPGKVVGEEQLAANHEQFMAFQSGDDVSAVDMPASDLQKKVKDKVQEAGKSKDQGPLQ
                     VIVEIVPDNIFSSFMDITKMLQVIFFAILFGVVLSMIPGDKAAPMIGFIESGNEVFLK
                     MVDVVMQAAPFFVFALMAGNMADLADSPAALFNLFKGLGMYALTVVLGLAFSLFVTYP
                     AVLRLFTRQIPYREFFRGMGSAQALAFSTSSSAATLPATMDCVTENLGVSRRIASFTL
                     PLGATVNMDGTSLYQAVSVLFLAQMHLVPLDLSQQLTIVVMATLISIGTAPVPGVGLI
                     MLIIVLESVGLNPMWIAIILPIDRPLDMLRTLVNITGDAAVTASVASTEGELQFQRKD
                     SIDNFDV"
     gene            14731..15447
                     /locus_tag="PK742_01450"
     CDS             14731..15447
                     /locus_tag="PK742_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQU75281.1"
                     /translation="MSSLWIITGTSSGLGKALAIQAASDDDALVYGIARREGPVLPRY
                     VHIHSDLSVAHNLLHLSLPEAGDAARIILVNNAATLGQVAPIGELDEAGIIGSYILNV
                     VAPHVLANKLLRTYRSSEAKKIIINISSGAATTPYDGWSIYSSSKAALNMQTLIGAEE
                     AGIREDADRFFAVAPGVLDTEMQATVRRSAREQFSRISKFTALFEEGKLADPAKAAAK
                     IIEIAAHPDDYTDTICRLSL"
     gene            15444..17072
                     /locus_tag="PK742_01455"
     CDS             15444..17072
                     /locus_tag="PK742_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQU75282.1"
                     /translation="MNYLFLIVSFIWAAPQQADMLLVNGRVYTVDSAFHLTQAIAVRD
                     GRILATGSTQEILSNYESDRILDLQGKTVLPGFNDGHCHFLNYGLYSGWAQLYGLTSM
                     DEVLDVIADMDTSLTAGWIIGRGWDQNLWSGKAFPDCRELDRLYPNTPVYLVRVDGHA
                     ALVNTYALKLAGIDKHTQVNGGKVFVEGDHCTGLLLDNAMDLFDEILPDKDPQVLSAA
                     LQKAQQDCFAFGLTSVTDAGLEKYQVDAVIREKQTGTLPVRMNIMLTPSQENVASFLM
                     QGPKISDNLVIRSLKVYADGALGSRGAALMEPYSDDPFNSGLLLIHPDSLNTLAALCA
                     SRGYQMCTHAIGDSANRLALKTYAAHIPAGNDLRWRVEHCQVVNPEDLHYFQDYQILP
                     SVQSTHATSDMYWAEERLGPERVKYAYAYQDMLEAAGVLINGTDFPVEQIDPLLTFYA
                     AVARMDQQRFPSGGFQTENALTRIDAIRSMTIWPAYGSFEESVKGSIEPGKFADLVVL
                     DKDIMSMPYPEIIRARVVMTILNGEVVFTNEQAD"
     gene            complement(17069..17413)
                     /locus_tag="PK742_01460"
     CDS             complement(17069..17413)
                     /locus_tag="PK742_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75283.1"
                     /translation="MKNLTYLIAAMLLCTVSLFLLPWYFVLIICFILGIIRGNKKYRN
                     AFLQGFLAVFLTYSVLYLFRDIPNEHMLSNEVAGIFLLPDGYVLLGAGALIFGILAGF
                     SWWSGAALRRLL"
     gene            complement(17416..19848)
                     /locus_tag="PK742_01465"
     CDS             complement(17416..19848)
                     /locus_tag="PK742_01465"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQU75284.1"
                     /translation="MKGWFHFLIPAALVTLAGFWLHAGHGSLPATGPLFDPVKGFMAQ
                     AEGTRPPVPDQIDLDGTDGPVHIYYDERLVPHIFAESEQDLYFAQGYVTAGLRLWQME
                     MTSLASSGRLSEKLGERFISYDRFQRRIGMVENARHAMASLPDDDPSKLAIQAYARGV
                     NAWISGLSYKDYPLEYKLMGFEPSEWTELNTYLLLQYMSNTLTGYDDDAAFTNLKQVL
                     GKDLYDLMYPERWAISDPIIPAGTSWSNPGPSAPDNPVSSAEISLYEEMPFQRGGEDQ
                     SGIGSNNWALSGSMTNTGKPILCNDPHLPLNLPSLWFEVQLVAPGMNVYGVSLPGSPC
                     VIIGFNESVAWGVTNAGWDVRDWYRITTSPEHPGKYAYEGGWEDLLMRVETIHVKGGE
                     DVLDTVWSSHHGPMVYLDLDSAHQSWNDLAMHWSAAEGGGELRTFYQLNKAGNAEDYR
                     KALEWFACPAQNIVFASDQDDIAITVTGKLPLKYQGQGRNILDGSKASDDWKGFIGFA
                     DNPWILNPDRGFVSSANQHPTDTLYPYYYTGFDFEYFRNHRINELLRSLDQAGVDDMM
                     AIQQDVYNSNAAEALPVMLQSVSKDDLTKADGFWLDKLKDWDYMNDPESELPTLFEIW
                     WKTFSNLLWDEVADRDNIDLTMPKPPVTIAALQWPDDMPIFDNRSTPETETRTIILQH
                     ALQKAVAEMDSLEALGNDATAWYKYKGTTVLHMAQLLPFSRPDVFVGGNRGIVNATTH
                     TNGPSWRMIVSLGDTPSAKAVYPGGQSGNPGSRYYDNFIDDWAAGKYYDLQYFRSAEE
                     ASAHAMLHLTIE"
     gene            complement(19853..20140)
                     /locus_tag="PK742_01470"
     CDS             complement(19853..20140)
                     /locus_tag="PK742_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75285.1"
                     /translation="MKKHLLPIALLAVTFMWSCKSKPVWPEADQKEFMDICEQAMYGA
                     FDRDTNFSYCACMLEKIMDEYPDPEQQDEVSDDLILEFADACFDELGIKMP"
     gene            complement(20236..21159)
                     /locus_tag="PK742_01475"
     CDS             complement(20236..21159)
                     /locus_tag="PK742_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75286.1"
                     /translation="MRPLPVIAILFAGASLLSCNKDVKDFITLYFDVNADAGLPRLDA
                     TGEEAVLPAGHVAVSPLQVRDIAVQYIELVPTEATPYKGGELVFKAPETYITGSLAID
                     FDSLLTGTAGSTVFTVNLRKLSPGTYAYARVGIAYLSYDIPLNISGLPGAEYLTDQQA
                     TFASFLGFNTYIREVQINQLTKVINDAKTGGYWVLETALEPPLDGENALFTWQVPQES
                     LTVVNILSGTSPVPPGQSVITGAFDEPLVVSEEEERDLRITFTLSVNGIFEFNDENSN
                     GAWDLHADNIYESEPIIDFGIRGLRPSFVFE"
     gene            complement(21156..>22203)
                     /locus_tag="PK742_01480"
     CDS             complement(21156..>22203)
                     /locus_tag="PK742_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQU75287.1"
                     /translation="LLGILGASRMTTDITLADGLPKHTAVYDDFLFFEEQFNGFRPFE
                     LAAIAGKGFEITDTVVLQQIDLVENYIASFPEAGNLFSVTAFYKVLNRALDGDNPAAY
                     RLPDNAPRWNRVQTLLGRYAGPDMQLYSSADGSMGRLSGSFRDAGTDQIGGIQDSIKQ
                     FIARSTDSNQVTFRLTGTGLMFDKNNEYIRRSILSGIVIALSVISLLMALLYRNFRML
                     VIAVVPNTIPLLICGGILGFFRIPLDAPTAIIFGISYGIVVDDTIHFLSRFRLEIARG
                     VPLEEAIRRTFSETGRALIMTTLILFFGFSVLMLSNLQATFNVGMLIGITLLSALISD
                     LYLLPLLLRKWYRP"
BASE COUNT         5907 a         5173 c         5571 g         5552 t
ORIGIN      
        1 accccgatat ttgcagtccg tttgtaaaaa gaaagcgtca tggcacgtgt atgtgactta
       61 acaggaaaga aaaccatcag cggcaacaat gtgtcgcact ctaaccgcaa gaccagaagg
      121 aagttctacc cgaacctcca gaccaagcga ttcttccttc cggaagaagg ccgttgggtg
      181 gagctgaaag tagctgcttc tacaatgcgt accattaaca aaaaaggtat tactgcctac
      241 ctgagcgagc tgcgcaagga aggcaagatc tgataatttt cagtagcaat ggcaaagaaa
      301 gcaaaaggct taaggatcca ggtaatcctg gaatgcactg agcagaagaa cacggatgta
      361 ccgggtatca gccgctacat cacgaccaag aaccgtcgga atacccccga ccgtatcgaa
      421 atgaagaagt acaatccgta cctcaagaaa atgactgttc acaaagaaat taaataacca
      481 tgggaaaggt cagtaagaac gcccgtgtta ccaagggcaa ggtttcaggt ggtggtaagg
      541 agatggtaaa ggtcatcgtg accgagaaat ctcctaaaac aggagcctat atcttcaagg
      601 aaaggatcgt acacaaagac aaggtacagg acgttctgaa aggtaacgcc tgatacacga
      661 acgatattcc agttaaaagc ttcttcgcaa gagaggcttt ttttgtttat agaaaaacgt
      721 gtttatggcg ttattcggac tgtttgggaa agaaaaaaag gaagaccttg acaaaggact
      781 ggagaagacc cgcgaagggc ttttctcccg aatctcgagg gcagtggccg gaaaagctac
      841 tgtagatgct gaatttctgg atgaactgga ggagatactc atcagcagcg atgtagggct
      901 ggacactacc gtcaagatca tcggccggct ggaagaacgg gtggctcgcg ataaatacct
      961 gaatactgca gaactccagc atatcctccg tgaggagatc gttcgcctgc tcacagacag
     1021 caacacgcct gactacgatg attttcagct tcccgcttcc gccgatccct atatcatatt
     1081 ggtggtagga gtgaacggag taggtaagac caccactatt ggtaaactgg ccagtcagtt
     1141 caaatcgcag ggtaaaaaag tattgctggg tgctgccgat accttcaggg ctgccgccat
     1201 cgatcagctc agtgtctggg ctgaccgcac cggcgtggag atcattcgcc atcagatggg
     1261 tgccgatcct gctgccgtag cttttgatac cgtgaatgtg gctaagaaca aaggagctga
     1321 tgtggtcctg atcgataccg ccggaagatt gcacaataag gtgaacctga tgaatgaact
     1381 ctctaagatc aaaagggtca tacagaaagt ttatcctgat gcaccacagg aagtattgct
     1441 ggtactggat gcatctaccg gtcagaatgc cctggaacag gccaggcatt ttacggctgc
     1501 aaccgaggtt actgccattg ctctcaccaa actggacggc acagccaaag gaggtgtggt
     1561 attgggaata gcagaccagt tcaggattcc tgtaaagtat atcggggtag gagaacaggt
     1621 gcaccagctt caggtcttca ataaaaaagc attcgtggat gcactcttcc atgaacccgc
     1681 atgaaggtaa ggtcgcacaa aaaagataag gtgaacatcg tgacactggg ttgttccaag
     1741 aacctggtgg acagtgaggt catgctgaca cagttgcggg ccaatgagat agatgcacac
     1801 caccagctgg aagacgacaa cagtaatgtc atcattgtca atacctgcgg gttcattgac
     1861 aacgccaaac aggaaagtat tgataccatc ctgcaatatg cttctgttaa atcagaagga
     1921 ggtatagata aattgtatgt gaccggatgt ttgtcacagc gctaccgcga tgaactcatg
     1981 caggaaatac cggaggtgga tgcctggttc ggtaccatgg agctacccgc actgctggag
     2041 aaatttcagg cagactatcg ccaggagttg ctgggcgaac gcatcattac tacggctccg
     2101 cataccgcct atctgaagat atcagaagga tgcaaccgca cctgttcttt ttgtgccatt
     2161 ccactcatgc gtggcgggca tcgctcccga ccaatggaag acctggtaac tgaagcttcc
     2221 agactggttg ctaatggcgt ggtagagatc atgctcattg ctcaggaact tacctactac
     2281 gggctggacc tgtataaaga aagaatgttg ccggagttgt tgcgacagct tagtgacatt
     2341 gaaggactgc gatggatcag actgcactat gcctatccga gtaagtttcc gcttgaggtg
     2401 atcgatgtga tgcgtgaaag aaataatata tgtaactatc ttgatatgcc attgcagcat
     2461 gccagcgact ctgtactgca tgccatgaga agacagataa cacggacaga aacagaggct
     2521 ttggttcatg agatacgcag aaggaatccg gagatcaccc tgagaactac tatgcttgta
     2581 ggctttccgg gcgaaacaga agaagacttt gaagaccttc ttgattttgt aagaaaaatg
     2641 cggttcaaca gattgggcgt atttacctat tctcatgaag agggaaccag tggctatgaa
     2701 ctggaagatg atgtacctga tgacgtgaaa caagagcgtg ccgcaaaact tatgtctgtg
     2761 caggaagaga tcgctgcaga atggaaccag acatttgtag gtaaagagct gacggtactt
     2821 attgacagac tggaaggtga acacttcgtt gggagaacag aaggcgactc tcccgaagtg
     2881 gacaatgaag tgctgatatc agtccataat caataccttc gcctcggcga ctttgtacag
     2941 gtgcacatca ccggcgctga tgccttcgac ctgtacgctg aaccggtgca gaaatgacgt
     3001 ttactcattc aaagataaaa ctggcagata ccggattctt tccgcagatc atgctggatt
     3061 atatggcagg tgcagaagca ctcaggccat tctaccaaca tacaccaggt cttgatgcat
     3121 ttggcgaggc aatgagcgcc aaggctcgtg aaggaacaga ccgttctaca ttggtgaagg
     3181 tactgcagga gcaattgcgt aacctgacat tgacagatgc agtaaaagat aatatacaat
     3241 cactggctga tgacaacacg ttttgcgtgg taaccgctca tcagctgaac cttgccacag
     3301 gacctcttta tgtgatctac aaagcactgt ctgctatttc actttgtgaa cagctggaaa
     3361 aacaatatcc gggtaaaaaa ctggtaccgg tattctggct tggatcagaa gaccatgatc
     3421 tggatgaggt aaaccacttt cacgccttcc ataagacata cacctggcaa accgaacagt
     3481 ccggtgcagt tgggcgcatg ctggccaccg gtatggatgc tttatgggac agcctggctg
     3541 aagatgcagg caactcttct gaagctgatg cgtgggtcaa catgcttcgt gattgttaca
     3601 gagcagacag aacaatgtct gttgcaacca ctgatctgct taacaggatc tttggaacat
     3661 atggcctggt gattctggac ggtgatcatc ccgcaatgaa aaaggcttgt gcagatatca
     3721 tcctggatga actgcagagc gggaactcag caggtatagt tcagcttgca tctgaaaaac
     3781 tttcccgaca ttattccgtg caggcttatc ctagagagat caacctgttt ttcctggata
     3841 acaacagccg tgatcgtata acaagggttg cagatggata tgaactggct aacagcagga
     3901 gaacctttgg cgctgaagag ctggaagaac tgatcaataa tcagccacag gtatttagtc
     3961 caaatgtgat cctccggcct ttgtttcagc aaagggtact gcctgctgta gcatacgttg
     4021 gcggagcagg cgaattatct tactggatgc agctaaaaac actttttggc caccacggta
     4081 tagaaatgcc ggtactggtg ctcagagact ctgtcaccct ggtggaccat acaagctgta
     4141 aaaaattgca gaaactgggt ctggaacctg atgctatgtt cggtgatatt gagcaactgg
     4201 ttaaagcata tgtgtctcag caagccggtg aggagatcga tctggagatt gaaaagaaac
     4261 aactggaaaa gacttacagg agcatctctg aaaaggcagc aaaggttgac cctacccttg
     4321 aaaaagcagt ggcggcggaa gaggcttcgg ccctgaaagc cctggaaaag ctgcatcagc
     4381 ggctgctaaa agctgaaaaa caaagatttg acgttgcgat caaccaatta cgctctgtac
     4441 atgcccgaat gtttccgaac ggaggtttgc aggaaagata tgacaacctg ctaccctggc
     4501 ttttgcgttt tggtccttcc tttccggatc agttgctgaa agttatggat gctccgtctg
     4561 atcatcttca tatactctgc gaagaacctc aggcctgatt atataccatt ctctgatagt
     4621 cacaaaaatg tagtagttta cactgcatct cctctgcaaa tgaaacattt ttgtatagaa
     4681 gaatggagtc gccttttgag cataccagga taacagaacg ttaccgcggt ctggagcatc
     4741 tgtctgtgcc agtagcactg gttgacgcct cctttaatgt tattcattac aatccggcat
     4801 acagaaaact tctgccacaa ggcgcaaaca agcagaagct gctgcacctg gacaggatgt
     4861 tcaacggatt tcaggacaaa cttaaagttt tgcaggatgc aggattgtat gccggcaaca
     4921 tcatgcaatt ccgaatgccc ggagtacagc tgtcagaaac caaccggata ttcgatctgc
     4981 acattgcccg cctggaacaa tctgcagatc tgctggatgg ctacacgatc aacctgatag
     5041 aagtaacgga aagggaacag gagctgaaga gactcagaga actttctgat catcacctta
     5101 aattcattca tcacaccacc agtggggtat tgattcactg caatggtaaa ctggtttatg
     5161 taaacccgca gggggtcaga atggtgcggg ctgcagatgc agagagtctg ataggtcgca
     5221 gtgtggttga ttttgttaca cctgagttca ggaccatcgt ggtagaacgg atccgcagaa
     5281 tgaacgaaga gaatcttcct gttgaagtca ttgaagagca tatcatccgc gaagacaaga
     5341 ctacttttcc tgcagagatc ttcgcttatc cggtaaccta tgacggagca ccggctataa
     5401 aggtgatcat caatgatatt actaaaagaa aagaggcgca gctccgtctt caggagagca
     5461 aacaggaata ccagaatctg atcgatcagt tacaggatgt gattttcaga gctgatgaaa
     5521 aaggcaagat agaatttctg aattcagcct ggactgaatt gaccggatac agagtggaag
     5581 aatgtctggg acattccatg agtgattact ttgtgaagaa cagcgacaca gacagctcat
     5641 ttgtgcatgt tatcaggcag ttgaacaacg gcatcggaga ctttgaaaat gatctgctgc
     5701 tggaaactgc aactggacaa catatatatg taagagctaa atttcagacc attcgcgatc
     5761 atgccggcct cctgacagga ttttcaggcc tgctcagtaa tattcatagc cgaaaaacca
     5821 tcctggaaga acagcgacgt attgaaaacg cacttcgaag aaaccagaat ctgtatttgt
     5881 cgctggctaa atcgatgcag gcaggaagca gaggattgca ggaagcctgc cgcgagatag
     5941 ccagaacatc atgtgtggcc atggcactgg atcgggtgaa tatctggcag ttcaatgaaa
     6001 caggaaatga gctgacttgc ctgacaaact atattaaaca agagcaaaca ttcgcagatg
     6061 aagggcttgt actttctgac ttttctgcct acacagagat catcagagaa gaacgggtgc
     6121 ttactattga tgatgccttg caagacccgc gtgtggatgt attcagagat agctatatca
     6181 ttccggatgg tattctctct atgatggatg caggaattta tattggagaa aagctgtggg
     6241 gtgtcatctg ttttgacgta acggagtccc gccgcgactg gatgcctgaa gaccgttcct
     6301 ttgctgctaa tctggcagat ctgttgtctg gtatcattgc cagtaatgag caggaggaaa
     6361 ccagatcagc gctaaagaag accgttgagc tgaacagcac cattctggaa aatgccggcg
     6421 agtccatatt cctgatcagt caggaaggac ttgtggaaga tgccaacaat atgacctcca
     6481 ggctaaccgg ttattctctg gatgaactga ctggaagttc catttccaga tttattccgg
     6541 aagagttcat ttcaacggga gactccgttg tgcgtaatat tgaaggaaca gggaccttcc
     6601 atcgtacccg gacactggtc tgtgctgatg gccgcacaaa aactgtggag atttcagtca
     6661 cgagactgga ggacagcagg atggttgcca tcatgcgtga catcaccgaa cgaatagaac
     6721 gtgaaaaagc ccttcgtgaa agtgaagcca ggctggacat ggctctgaaa ggcgctgatc
     6781 tgggtacctg ggatttcttt atcaaagaaa accgcatcat tcacaacagg cgctggggcg
     6841 aaatgctcgg ctaccatttt gagatcactt ctgtcaatga acagttctgg gaaaagtttg
     6901 tgcaccctgc tgatattgaa tcttcctaca ggaagtttca ggatcatatt aatggattga
     6961 caccattcta tgaagccaac ctgcgaatgc tggccagtaa cggcgagtgg aggtggatac
     7021 ttgacaaggg acgtgtggta gagtgggatg agaacggaga acctgtcagg gcttccggta
     7081 ttcatcagga tataactgcg ttaaagtcta ttgagaaaga gattgaagat cagcgcatat
     7141 ttctgcagca gatcataaat gccataccag accccatcta tgtgcgtaat gcaagcgatg
     7201 aatttgtggt catcaacaat gcctttgctg aatacctggg tgttgacagg cagatggtca
     7261 tgcagtataa attcaacagg aaccatcagt ttcatgaaac atttcagcaa ctgatagatc
     7321 ccgatgcaga gatcctgctg aaccagcagc cactggagct gaaggaacac aaactgctga
     7381 atcaggctac gggaaaacat acctgggtaa gcactatgaa ggtgccgctt accgatgctg
     7441 atggacaggt tctggaagta ctgagcatca gtacagatat atcagatctg aagaacagag
     7501 agacttccct gaaagaaaaa gtagaagacc tgtcagaaaa gctgcgtaac agaaccggca
     7561 aactggagca gctgaccaaa gagatcgaca ctttccatta ttcggttagc catgacctga
     7621 gaaccccgct tcgcactatt gatatttttg cctacttcct ggagaagcgg tacagtgata
     7681 agctggatga agaaggaaaa tccaatatta cgcagatcag gaacagcatc actaaaatgg
     7741 gcacattgat tgataatctg ctcatttata tcagaatggg gaaggtgccg gtcagaaaaa
     7801 aaatggtaca ggtttctgat cttgtgcagc aggtcataga tgaaatgggg cgcagtaccg
     7861 acctggaaca ggtcagtttt gagataggcg ggctaccggt catggagacc gatccggata
     7921 tgctgcgcca ggctttgcgg aacctgatca gtaatgctgt gaagttttca gcaactgctc
     7981 gtcagccggt ggtgcagttt tcttcttcca aagtggaagg ttacagtatt atccggatca
     8041 ctgataacgg ggtgggtttc aacatggagt ttaaagacaa gttattcaaa gcattcaaaa
     8101 ggcttcacag cgaagagcac tttgaaggaa ccggtgtggg gctggccatt gttgaacgta
     8161 tcgttcagcg tctgggcggt gaaatatggg cagaaagtgt ggaacaagaa tataccacct
     8221 tttttataaa attgccgttg cattaatcgt tttccttgtt aactttgcga accgcttttg
     8281 cgggtagttg ccgagatggt gaaactggta gacacgcacg tttcaggggc gtgtggccgc
     8341 aaggctgtgt gggttcgact cccactctcg gcacagaaca aaaaaggctc cctaaaggag
     8401 ccttttttga tttatgcagg tctttatttt ttcgtgatca ggccttgtct ggtcttaccc
     8461 tgacaactgg tttgccaggt gtaggttccg gcagacaggt gactgatatc taccgatgcc
     8521 ggaatgccag cttccagcca gccggtttcc atttccatta ccatttttcc attcagatcg
     8581 aacagccgaa cctgataaat gcctgcatca gaagagctgt ttgtgatatg cagccagccc
     8641 gcagttggat taggatacac ctctccctga tcttctccac ttgttctcat gggcggagtg
     8701 gtaaaggtga tcacattgga acggagtgtc caggtggtgc cacatttggt ctgcacccag
     8761 cttttgtatt tcttggaagg ggtgagtccg gtaagggtat aggagtcatt cagactgaga
     8821 tacaaatggc tttcacccgc acctttgacc gttaccttat aaatggtggc atcatctgca
     8881 cccgtccatg aaacatctgc tgtggttgga gccagtgagc caactgcata ggatgatggg
     8941 gtagggcagc tgtttaagcc atgaaaaaca aaagctgaac cttcgtaggt ttccggatta
     9001 ctgtatttga aagctccgat gatgacatca tcaaaaccat cgtggttgac atcgccggca
     9061 ccatcgcagg tccatccgaa gtttgcctga ttcacaccac ccaggtaggt ccatgctgct
     9121 gttgcagaca aacctgacgg cccgcccaga tagagatagg ccttgccttc cttgttaaag
     9181 tcttctgtat gaaagtgtga tcccatgatc acatctgcat aaccatcgtt attgacatcg
     9241 ccggcaggtc cgaactgaat ggcatactca gcgccgtcat tatctccttc agccgtccag
     9301 ctggcagttg tttcaggccc agcggggctt cccatgaata gccaggcttt cccttcatct
     9361 acaaattctg catcatatct cttggcacct atcatgatgt cgtcaaaacc atcattgtcc
     9421 acatcgccca caccggatac ccagaaagcg aaatacccgt tgctttggtc tccttccagt
     9481 tcccatacag gactatcggc cagaccggtc ggtccaccca gaaacaccca ggctttaccg
     9541 cagttggcta tagctcctga ataattccat ccgcctatga tggcatcgtc atatccgtcg
     9601 ccattcacat cgccggcagc actgactgca tggcccagcg cagcgtcagt attgttacca
     9661 tcaacggtcc agtccggttc atcggcaaga ccatcggcag aaccataaaa tacgtatgct
     9721 cttccttcat caacctgatc gccgtcccag ccattgcctc caatgatcac gtcatcataa
     9781 ccatcattgt tgacatcacc taaaccttcc agcggaaatc cgagacccac acccgcctgg
     9841 tttggttcaa accaccacac aggttcagtg gttacaccgg tggaggtgcc ataatacaga
     9901 tgtgcccggc cttcttctga ttcgccaccg ctgtaaagac cgaggcctat caacaaatcg
     9961 tcatagccat cgccgttcac gtcgccggca gctgccacag acttgcccat taatccgctt
    10021 tcgttatcac cttcatcttc ccagacagga gtggtggaca aaccggatgg accgcccaga
    10081 aacaaaaatg cttttccttc gtcggtttca cctgcatcat aggctgcagc gccaactacc
    10141 acgtcatcat atccgtcatt gttgacatcg ccggcagtac tgacagcaaa tgccatgtta
    10201 gcgcctacct ggttgccttc gtagctccag tcaggagtag ttgtgagcgg gtctatggta
    10261 acaggaaaca cagcattcct ggtgtctgtt accagcgcca gttcctgacc gttcagctcc
    10321 atgtgtgatt ccagagacct tccataagca tcatacgccc ggaggttgcc gtaactgaat
    10381 aatgtctgtc ctcctgccac cagctcatag gaattgttgg tcatacggga ttctccgcta
    10441 ccgctcatat gcagcttcac agctacccgg gcagcaggtt cgcccggatc ttcaatgatg
    10501 aaattctgtc tgaaaccttc gggggcattc atgtattcta tctggtaagt tccggcgtcc
    10561 attaccagca attcagcact ttctgtctgc cagttgattc cggtggaagc caatggcagg
    10621 ttatcagcca caacctgatc aagggttact tccaaccgga catcagcgtc tgatcttttc
    10681 actgctttaa aacctgattc agtcaggtag aatctgctgt cacttcttct gttgaatgct
    10741 tcaaaaggat tttctgttcc ttttctgaac tgatattcag aggccatgat ctgctgggtt
    10801 accagcattt gccaggtatc tgtttcaggg tctttggcgg tctggcttgc ctgggtcgga
    10861 gagatctgtt gatacaatac gatgcctgat gccagcacgg cgatcagaac tgttgagagg
    10921 gttagttggc gtgacatatg cataaattgt gtacaaaagt acgggtatat ttttgattcc
    10981 ttctgtcagg agattgtcgg taaaatggtt ctttcaatat gatctccccc gaatgcatag
    11041 ggtaaatatg ccagatgctc cattggccgg gtgatgatga gccttgcgtc tttaccggca
    11101 gtgatgctgc ctgcacggtc gctgatctgc atggcagcag ctccgtttac tgttactgcg
    11161 ttgatggctt cttcaggtgt caggcgcatc ttgatacagg ctagtgacaa aacgaaggga
    11221 atcttaccgg aaggagtgga gccggggttg taatcggtag ccagaaccag aggtaaaccg
    11281 gcatcaatga tgtccctgcc aggcgcataa ggcagattaa gaaagaagct gcaggatgga
    11341 agagctgtag gcagggtgtt gctgtccagc aaacagtcga tctcattgcg ggtgatatgc
    11401 tccagatgat caacagatat tgcattgttt ctgacgcctg cctgaactcc gcctgaaact
    11461 gcaagttgat tggcatgaat acgaggtgca atatcatatt tccaggcagc ctgtagtatg
    11521 gtgtctgttt cttccggcgt gaaaaatcca ttttcgcaaa aaacatcaca gtaatcggca
    11581 agaccttcat cagcaatctg tggcaacatg acatcagtga tcagcttgat gtatccgttc
    11641 ctgtcttgcc tgtattctaa cggcagtgca tgagcgccca ggaaggtaga tctgatgatc
    11701 agtctgcttt gtgatctcag tttctgtatg acccgaagta tcttgagttc tgcatcgacg
    11761 gttagtccgt aaccgctctt gatctctacc gctccggtgc ccatgcgttc tatttctgcg
    11821 agtctttcga gtgcagaagc ttccagctca tctgctgatg cctgttgtaa ttttcgggca
    11881 ctgttgagaa tgccaccacc tgcagctgcg atctcttcat aggatcttcc gcggatgcgc
    11941 atttcaaatt cttcttctcg gctgcgggca aaaactagat gggtgtggct gtcgcaaaat
    12001 gccggcaata cccaccttcc ggtggcgtcg atgacctcat cagcttcagg aagttcctga
    12061 tccatcgtgc catatgcagc aatgcgcccg ttctcagata gcagccatgc attgctgagg
    12121 gttgggaaaa gattcatgtc tttgcctgca agcctgtctg ttccatcagg ccagaccggt
    12181 ccaagcatat gaatttgttg aataagaatg cgatgggaag gcatcttcaa aggtacggac
    12241 gcccgattac aaatcaaacg caacctgtat cttcgcagga tgccgggtaa ttcttttgga
    12301 acatattttc gcatcactac atttggtgaa tcacatggac cggcaattgg tgtggtcata
    12361 gacggatgcc cgccgggcat tgcattgaca gaagcacaga tagatgaagc actggctcgc
    12421 agaagaccgg ggcaatcacg cattaccacc caaagaaaag agcccgatac ctgtgaattt
    12481 ctgagtggtg taatggaggg taaaagcacc ggaactccac tggcaattct gatacgcaac
    12541 agtgatcagc gttcgaaaga ttacgatcat cttgcagata cctacagacc ttctcatgct
    12601 gacttcacct accaggaaaa gtatggcatc agagatcacc ggggtggtgg cagatccagt
    12661 gcaagagaaa ccgcagcaag agttgctgca ggtgccgttg ctatggctgt tttaaaaacg
    12721 gaaggaatac atgtacaggc ttatgtaagc gcagttggtg atatcgtgct ggatgcatct
    12781 tatcaggatc tggacctcag tcggaccgat gagaatatca ttcgttgtcc ggatcaggaa
    12841 actgccgaaa gaatgatcag cctggtaaat gcggtccgga aagaaggaga taccattggt
    12901 ggtgtcatta cctgtgtggc atcaggtgtg ccctccggct tgggtgaacc ggtgtttgat
    12961 aagttacatg ctgacctcgg gaaggccatg ctcagtatca atgccgccaa agggtttgaa
    13021 atcggaagcg ggtttcatgg tgccggtatg cgtggaagta cgcataacga catttttgaa
    13081 aaccgtgatg gaaagatcgt tacccgcacc aatcattcgg ggggcattca gggtggcatc
    13141 agcaatggca tggatatata tttccgcact gcatttaaac ctgttgctac tattttaaga
    13201 cctcaggaaa cggtgaacag tgccggccag aaagtatctg ttgaagggaa aggcagacac
    13261 gatccttgtg tattgcccag ggcagtacct atcgtggagg cgatgaccgc catggtgctg
    13321 gcagaccatc tgctgttaca acgcatcaac cggaaatgat atgagaaaga ttgcgctaca
    13381 ttggaagatc ctgttcggac tgttactagg tgtcatatgg gccttactga gtgcaggact
    13441 cggctggagg caattcacgc tggactggat caagccctgg ggaacgatct tcatcaatgt
    13501 gttgaaactg atagccgttc ctttggtgtt attcagtatc atcaagggta tcacagatct
    13561 aagtgatatc agtaagatca gaagggttgg cttaaagacc attggtatct atgtaggcac
    13621 taccgtcatt gccataacag tggggctcgt ggcagccaac ctgatacagc cgggaaaggt
    13681 tgtaggcgaa gaacagctgg cagccaacca tgagcagttt atggcctttc aatccggcga
    13741 tgatgtttca gcagttgaca tgccggcaag tgatctccag aagaaagtaa aagataaggt
    13801 acaggaagca ggcaagtcaa aggaccaggg ccctcttcag gttattgtag agatcgtacc
    13861 agataacatc ttcagcagtt tcatggatat taccaaaatg ctgcaggtca tcttctttgc
    13921 aattctgttt ggggtggtgc tcagtatgat acctggtgat aaggcggctc ccatgatagg
    13981 ttttattgaa tcaggaaatg aggtctttct gaaaatggtt gatgtggtca tgcaggccgc
    14041 accatttttt gtgtttgcac tcatggccgg taacatggca gatcttgctg atagtccggc
    14101 agccttgttc aacctgttca aaggtcttgg tatgtacgct ctcacagtgg tattagggct
    14161 cgctttttct ttgtttgtta cctatccggc agtattgagg ttgtttacca ggcaaatacc
    14221 ctacagggag tttttccgcg gaatgggttc tgcacaggcg ctggcttttt ctaccagctc
    14281 cagtgcagcc accttaccgg ctaccatgga ttgtgtaaca gagaacctcg gggtatctcg
    14341 tcgcattgcc agttttaccc tgccgctcgg tgcaacggtc aatatggatg gtaccagttt
    14401 gtatcaggcc gtttctgtgt tgtttctggc tcaaatgcat ctggtgcctc ttgatctgtc
    14461 tcaacagctt accatcgtgg tcatggctac cttgatcagc ataggtacgg ctcctgttcc
    14521 gggtgtggga ttgatcatgc tcatcatcgt actggaatct gtcgggctaa accccatgtg
    14581 gatcgcgatc atcctgccca tagacagacc tcttgatatg cttcgaaccc tggtcaatat
    14641 taccggcgat gctgcagtta ctgcatcagt ggccagcaca gagggagaat tgcagttcca
    14701 aagaaaagac agtatagaca attttgatgt atgagttcgc tgtggatcat taccggtacc
    14761 agcagcggtc tggggaaagc ccttgctatc caggcagctt ctgatgatga tgccttggtg
    14821 tatggaatcg ccagaaggga aggccccgtt ttgccacgat atgtgcatat acattctgat
    14881 ctgtctgtgg ctcataacct gcttcacctg tctctgccgg aagctggtga tgctgccaga
    14941 ataattctgg tcaacaatgc ggccacactg ggacaggtgg ctccaattgg cgaactggat
    15001 gaagccggca tcattggatc ttatattctt aatgtagtgg cacctcatgt gctggccaat
    15061 aagttgttga gaacctacag atcatctgaa gcgaaaaaga tcatcatcaa tatttcttcc
    15121 ggtgcagcca ctacacctta tgatggctgg agtatataca gcagcagcaa agctgctctg
    15181 aacatgcaaa ctctgatagg tgctgaagaa gccggtatcc gggaagatgc tgacaggttt
    15241 tttgctgtgg cacccggtgt actggacact gaaatgcagg caacggtcag gcgttcggcc
    15301 cgtgaacaat tttccaggat ttcaaagttc accgctttgt tcgaagaagg gaaactggct
    15361 gacccggcca aagcggctgc gaagatcata gaaatagccg cccatcccga tgattataca
    15421 gatacaattt gcagattgag tttatgaatt atttattttt aattgtttca tttatctggg
    15481 ccgcaccaca gcaggcagat atgttgctgg tcaacggcag ggtgtacact gttgacagcg
    15541 cttttcattt aacacaggca attgcggtca gagacgggag gatcctggca acgggttcta
    15601 ctcaggagat actgagtaac tatgagtcag acaggatact ggatctgcag ggtaagactg
    15661 tcttacccgg tttcaatgac gggcattgtc atttcctgaa ttatggcttg tacagcggct
    15721 gggcacaact atacggtctg acaagtatgg atgaagttct ggatgtgatt gctgacatgg
    15781 ataccagcct tactgcaggc tggatcatag gaagaggatg ggatcagaat ctgtggtcag
    15841 gcaaggcatt tcctgattgc agggaactgg acaggttata ccccaacaca ccggtatatc
    15901 tggtgcgggt agatggccat gctgcactgg taaatacata cgccctgaaa ctggcaggta
    15961 ttgacaaaca tacacaggta aatggcggta aggtctttgt ggaaggcgat cattgtacgg
    16021 gtttgctgct tgacaatgcc atggatctgt ttgatgagat cctgcccgat aaggatccgc
    16081 aggtgctttc cgccgctttg cagaaagcac aacaggattg ctttgcattc ggtctgacct
    16141 ctgtaacaga tgccggcctg gagaaatatc aggtggatgc ggtcatcaga gagaagcaga
    16201 caggtacatt accggtacgc atgaatatta tgcttactcc ttcacaggaa aacgtagctt
    16261 ccttcctgat gcagggaccg aagatctctg ataatctggt gatcagaagc cttaaggtgt
    16321 atgctgatgg ggcacttgga tcacgtggag cagctttgat ggaaccatat tccgatgatc
    16381 cattcaacag cggtctgttg ctcatacatc cggactcgtt gaatacgctt gctgctctgt
    16441 gcgcttcacg gggttatcag atgtgcaccc atgccattgg cgatagtgcc aacaggcttg
    16501 ccctgaagac ctatgcagct catattccgg cgggcaatga tcttcgatgg agggtggaac
    16561 actgccaggt ggtgaaccct gaagacctgc attattttca ggattatcag atactccctt
    16621 ctgttcagtc cacccatgcc accagtgata tgtattgggc tgaagagcgc ctcggaccag
    16681 aaagggtcaa atatgcctat gcttatcagg atatgctgga agctgccgga gtgcttataa
    16741 acggtactga tttcccggta gagcagatcg atccgctgct gacattctat gctgctgtag
    16801 ccaggatgga tcagcaacgt ttcccttccg gcggttttca aacagagaac gcacttacca
    16861 ggatcgatgc cattcggtct atgaccatct ggcctgcata cggttctttt gaagagtctg
    16921 tgaaaggcag tattgagcca ggtaagtttg cagacctggt ggtgctggac aaggatatca
    16981 tgagtatgcc ttatccggag atcatacgtg ccagggtggt catgaccatt ctgaatggag
    17041 aggtggtctt tacgaatgaa caggcagatt agagcaatct tctcagcgca gcgccactcc
    17101 accagctgaa acccgcaagt attccgaata tcaaggcacc ggctcccagc aggacatagc
    17161 catcaggcag caggaagatt ccggctactt cattgctgag catatgttcg ttcggtatat
    17221 ccctgaacag atataataca gagtatgtga gaaatactgc caggaatccc tgcagaaagg
    17281 catttctgta cttcttgttt cccctgatga tacccagaat aaaacagatg atgagcacaa
    17341 agtaccaggg cagtaagaac agacttactg tacacagtaa catagcagct atcagatagg
    17401 tcaggttctt catggttatt caatagtgag gtgtagcatg gcatgtgcag aagcttcttc
    17461 ggctgacctg aaatactgaa gatcgtaata ctttcctgct gcccagtcgt caataaaatt
    17521 atcatagtac ctgcttcccg gattcccgct ttgtccaccc ggatacacag ctttggcaga
    17581 tggtgtatct ccaaggctga cgatcatcct ccaggaagga ccattggtat gggtggtggc
    17641 attgacgatt ccgcggttgc cacccacaaa tacgtcgggt ctgctgaatg gcagtaattg
    17701 tgccatatgc agcacggttg ttcccttgta tttataccag gcagttgcat cattgcccag
    17761 ggcttccagt gaatccattt ctgccacagc tttctgaagg gcatgctgta atatgatggt
    17821 tctggtttct gtttcgggcg tggaccggtt gtcgaagatg ggcatgtcat ccggccattg
    17881 cagtgctgct atggttacag gaggtttcgg catggtgaga tcgatattat ctctatcagc
    17941 tacctcatcc cacagcaggt tactgaaagt cttccaccag atctcgaaaa gggtagggag
    18001 ctcactttcc ggatcattca tataatccca gtctttcagt ttgtccagcc agaaaccatc
    18061 ggcttttgtc agatcatcct tgctgactga ttgcaacata acaggtaaag cctctgctgc
    18121 attgctgttg taaacatcct gttgaatggc catcatgtca tccaccccgg cctggtccag
    18181 acttcttagc agttcattga tgcggtggtt gcggaagtat tcaaaatcaa agccggtata
    18241 ataataagga tacagggtat cggtaggatg ctggttggcc gaactgacaa atccacgatc
    18301 aggattgagt atccacgggt tatctgcaaa accgataaag cccttccagt catcagatgc
    18361 tttgctgcca tccagtatgt tgcgtccctg cccctgatat tttagcggaa gttttcctgt
    18421 tacggtgatg gcaatatcat cctgatcaga ggcgaatacg atgttttgtg ccgggcatgc
    18481 aaaccactcc agtgccttgc gataatcttc tgcattgccg gctttgttca gctgataaaa
    18541 tgtgcgcagc tctccaccac cttcagctgc cgaccaatgc atggcgaggt cattccagct
    18601 ctggtgagca gaatccagat caagatagac catcgggccg tggtgtgagc tccagacagt
    18661 atccagcaca tcctcaccac ctttaacatg tatggtctct acccgcatga gcaggtcctc
    18721 ccatccacct tcataggcat attttccggg gtgttccgga gaggtggtga tacggtacca
    18781 gtcacgaaca tcccagccgg cattggtgac gccccaggcc acactttcgt tgaatccgat
    18841 gataacacag ggagagcccg gaagggaaac cccgtaaaca ttcattccgg gtgcaaccag
    18901 ttgtacctca aaccaaagac tgggaagatt gagaggcaga tgcgggtcgt tgcacaaaat
    18961 tggtttgccc gtattggtca tgctgcctga caaagcccag ttattgctgc caatgccaga
    19021 ctggtcttct ccgcctcgct gaaacggcat ttcttcatac agggaaatct ctgcagagct
    19081 gaccggatta tctggtgcag aagggccggg gttgctccag gatgttcctg ccggaataat
    19141 ggggtcactg atagcccatc gttcgggata catcagatca tataagtctt ttccaagtac
    19201 ttgtttcagg ttggtaaagg cggcatcatc atcgtatccg gtaagggtgt tggacatata
    19261 ctgtaacaac aaatacgtat taagctctgt ccattcagaa ggttcaaatc ccatcagttt
    19321 atattccagc ggataatctt tgtagctcaa tccggagatc caggcattga cacctctggc
    19381 gtatgcctga atggcaagtt tgcttgggtc atcatccggc aaagacgcca tcgcatgccg
    19441 ggcattttca accattccta tgcgccgctg aaaccggtca taactgatga atctttcacc
    19501 cagtttttca ctcaacctgc cggaagatgc caacgaggtc atttccatct gccacaggcg
    19561 taaaccggca gtaacataac cttgtgcgaa atacaggtcc tgttcagatt cagcaaatat
    19621 gtgcggaacc agtcgttcgt cgtaataaat atgaacaggg ccatccgttc catccagatc
    19681 gatctgatcc ggaactggtg gtcgtgtacc ttcggcctgg gccatgaaac cctttaccgg
    19741 gtcaaagagc ggtccggtgg ccggtaatga tccatggcct gcatgcagcc agaatccggc
    19801 cagggttacc agagcggcgg gtatcagaaa atggaaccat cctttcatca ggtcagggca
    19861 tttttattcc cagttcgtca aaacaggcat cggcaaattc caatataaga tcatcagata
    19921 cttcatcttg ttgttcagga tccggatatt catccatgat cttctccagc atacaggcac
    19981 aataactgaa attggtatcg cggtcaaaag ccccgtacat agcctgttca caaatgtcca
    20041 tgaattcctt ctggtcagct tccggccaga cgggttttga tttgcatgac cacataaagg
    20101 tcactgccag caaggcaatt ggtaaaaggt gttttttcat catttgcgca tgaagataat
    20161 actaaagaag acagttcact gacctgcaga agtaagaaca actggcgaca tgcagttcac
    20221 tcaggcctgt aatggtcatt caaatacaaa agatggcctg aggccgcgga tcccgaaatc
    20281 tatgataggt tcagactcat aaatattgtc ggcatgcaga tcccatgcgc cgttgctgtt
    20341 ctcatcatta aattcgaaga tcccgttgac cgagagggta aaggtgatgc gcagatcacg
    20401 ttcttcttct tcagagacca ccagaggctc atcaaatgca ccggtaatga ccgattgacc
    20461 gggaggcaca ggagacgtgc ctgacagaat attcaccact gtgagtgatt cctgtggaac
    20521 ctgccaggtg aacagggcat tttctccatc aagaggtggc tccagcgctg tttccagtac
    20581 ccagtatccg cctgttttgg cgtcattgat gaccttggtc agctgattga tctgcacttc
    20641 tctgatgtat gtattgaatc cgagaaatga agcaaaagtt gcctgctgat ctgtcagata
    20701 ttcagcgcct ggcaggccgg atatattcag gggtatatca taagataaat aggcaatacc
    20761 taccctggca tatgcatatg tgccgggcga gagtttacgc aggttgaccg taaacacagt
    20821 gcttccggca gtaccggtaa gcaggctgtc aaaatcaatg gccagcgatc cggttatata
    20881 agtttcaggg gctttgaaaa ccagctctcc acctttatat ggagtagctt ccgtcggtac
    20941 cagttcaatg tattgtaccg caatatcacg cacctgaaga ggagataccg ccacatgccc
    21001 tgccggcaga acagcttctt cacccgttgc atccagcctg ggcaatcccg catcagcatt
    21061 cacatcaaaa tacaatgtga tgaaatcttt aacatccttg ttacacgaca gcagggaagc
    21121 tccggcaaac agaatggcaa ttactggaag aggtctcatg gacggtacca ttttcggagc
    21181 aataaaggta gtaaatagag atcgctgatc aaggctgaca aaagcgtgat accaattagc
    21241 attccgacat tgaaggtggc ctgcaggttg gacaacatca aaactgaaaa accaaagaac
    21301 agtatcaggg tggtcatgat caaagccctg ccggtttcac tgaaagtgcg tctgatggct
    21361 tcctcaagag gaacacctct ggctatctcc agtctaaacc tcgacagaaa atgaatggtg
    21421 tcatctacca ctataccata tgatattccg aatatgatgg cagtgggagc atccagcggg
    21481 atcctgaaga aacctaatat cccgccgcaa atgagcagcg gaatggtatt gggtaccacg
    21541 gcaatgacca gcatcctgaa atttctgtac aacagcgcca tcagcagact gatcacagac
    21601 agcgcaatga cgataccaga caggatactt cgcctgatat attcattgtt cttgtcaaac
    21661 atcagtcccg tgccggtcag tctgaatgtg acctgattgc tgtcagtaga acgagctatg
    21721 aactgtttga tggaatcctg gatcccgccg atctgatcag tgcctgcatc acggaaagaa
    21781 ccactaagcc ggcccatact gccatctgct gaactatata attgcatgtc aggacccgca
    21841 tatctgccaa gcaaagtctg gaccctgttc caccttgggg cattgtctgg cagccggtat
    21901 gctgccgggt tgtcgccgtc cagggctctg ttgagcacct tgtaaaaggc agttactgaa
    21961 aacagatttc ctgcttccgg aaaagaggca atgtagttct ctaccaggtc gatctgctgc
    22021 aataccactg tatcagtgat ctcaaagcct ttgccggcaa tggctgccag ttcaaatggc
    22081 ctgaatccgt tgaattgttc ttcaaaaaac aaaaagtcat catacacggc ggtgtgtttc
    22141 ggaagcccgt cagccagcgt aatatcagtg gtcatcctgg atgcaccaag aatacccagc
    22201 aaa
//
LOCUS       DAOMAX010000011        21886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_245_length_21886_cov_4.548853, whole genome shotgun sequence.
ACCESSION   DAOMAX010000011 DAOMAX010000000
VERSION     DAOMAX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21886
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_21886_cov_4.548853"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK742_01485"
     CDS             <1..614
                     /locus_tag="PK742_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75288.1"
                     /translation="VISTGVTYSALISSATDKDWYKFTTSAPNTKIKVTLTSLPADYD
                     VRLYRGNSQKGISENAGTADEVIIWNTNSQGARYVYVYGWDGAFDAADCYDLLVEASS
                     SSWRTDGSETIIDEEWNNAIVGIFPNPASSVATVNYFSIQENTQVFVTVTDILGNKVM
                     TSQASVTSGDNLIELQVQDLASGIYLVDVSNGKSHYTEKFVKE"
     gene            763..1791
                     /gene="pheS"
                     /locus_tag="PK742_01490"
     CDS             763..1791
                     /gene="pheS"
                     /locus_tag="PK742_01490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQU75289.1"
                     /translation="MQDVFDKVAEVRKQIEEAAIGSSVELEAFRVRFLGSKNILKDLF
                     SAIKEVPNERKKEFGQLINEVKSLAESVFQEKSVLFADTNKQSARQIDLSLPAVTRNQ
                     GAMHPITLVREEIVHILERIGYVVAEGPEIEDDWHNFTALAMPDNHPARDMQDTFYIQ
                     TNPDILLRTHTSNVQIRVMEKQEPPIRIIAPGRTYRNETISARAHCIFHQVELLYIDE
                     HVSFADLKQTLLYLAQELFGSQTKIRLRPSYFPFTEPSAELDVSCFICNQQGCNICKH
                     TGWVEIGGAGMVDPAVLEACSIDPDKYSGFAFGMGLERMAMLKWQINDIRLFSENDLR
                     FLQQFEGR"
     gene            1893..2885
                     /locus_tag="PK742_01495"
     CDS             1893..2885
                     /locus_tag="PK742_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HQU75290.1"
                     /translation="MSRMTPRRVANMVKVISSYYYSKYTKKTVQWGVPISISIEPTTS
                     CNLRCPECPSGLRSFTRPTGMLKSDFFRQTIDALHKDLTYLIFYFQGEPYLNPEFLEM
                     VKYAAGKRIYTATSTNAHYLTEENARKTVESGLDRLIISIDGTTQEVYEQYRVGGKLE
                     KVLEGTRQIIRMKKEMKSSTPHVIFQFLVVRPNEHQIGDVKKLAKELGVDEVRLKTAQ
                     IYDFEDGSDLIPLQTKYSRYRKLATGKWEIKNPLNDHCWKLWHSCVVTWDGLVVPCCF
                     DKDAQHRLGDLKLTPFREIWQAWPYQEFRASVLKGRSQIDICRNCTEGLSVWKE"
     gene            2890..4095
                     /locus_tag="PK742_01500"
     CDS             2890..4095
                     /locus_tag="PK742_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQU75291.1"
                     /translation="MTELQPLFLRHLAQTSDEPLLLEITHAKGCVLFGPQERSWLDLI
                     SGISVSNMGHGHPDIVQAVQEQAAKYMHVMVYGEFVESPQVQYATWLANHLPGEIDSV
                     YFVNSGSEAVEGAIKLAKRATGRTEIVSFQHGYHGSTAAALVTGGNEERKRAFRPLPP
                     DNLILPFNDINSIGQISHRTAAVLVELIQGEAGIRPADEAFIQELAYRCRVVGALLIV
                     DECQTGFGRTGKMFAAEHYDLKPDIMILGKALGGGMPLGAFAAGKDLMQKLTTQPVLG
                     HITTFGGHPVCCAAGLASASLITTELLQQVEEKGKFLEEKLHHTAIKEIRRRGLMMVV
                     DLPDEPFNKAVIRRMITKGVVTDWFLFHDNGLRIAPPLVITVPELEFAARLLLESLDE
                     VQKELHSVN"
     gene            4110..5084
                     /locus_tag="PK742_01505"
     CDS             4110..5084
                     /locus_tag="PK742_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HQU75292.1"
                     /translation="MRSFSIILLTVLSLQASSAGAPWGFWGHQRINRMALYTLPEELF
                     AFYKPHADYVEEHAVDPDKRRYAVVEEAPRHYIDIDHYGVHPFEGLPHKWSEAVEKYS
                     EDTLQAYGIVPWHVELMMYRLEDAMREQDAKKIIRLTAEIGHYIADAHVPLHCTENYN
                     GQLTGQNGIHGFWESRIPELLGEDYDYFVGKATYIPFVRDKIWDIVLTSHLMSDTVLH
                     MEAMLDKEFPADRKYTYETRGQSTVRTYSKEYSIAYNASINNMVERRMRETIINLGSF
                     WYTAWINAGKPDLTGLEWTPYTDDELRDMEAMDKAYRSGGGMKGRSEE"
     gene            complement(5085..5357)
                     /locus_tag="PK742_01510"
     CDS             complement(5085..5357)
                     /locus_tag="PK742_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75293.1"
                     /translation="MEKRPPLIVCLLLDLFGYFSYSIPVLGEWTDIIWAPVAAAIYLH
                     LFGSRRGWIGAFLTFTEEALPGTDFIPSFTLSWILQRVTSRQTVRA"
     gene            complement(5459..5902)
                     /locus_tag="PK742_01515"
     CDS             complement(5459..5902)
                     /locus_tag="PK742_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75294.1"
                     /translation="MNSSSIISLSALLLLAGLTGCTSDTPDVTTTEVESTVTSGVWKI
                     TYFYDTDKEETAHFNGYEFTFGEDGTLTASNGASTVTGTWSVTDNSDDDSPSNPDFNI
                     FFASPDDFEDLSDDWDIISRTDTKIELQDVSGGNGGTDDLIFEKL"
     gene            complement(5913..8201)
                     /locus_tag="PK742_01520"
     CDS             complement(5913..8201)
                     /locus_tag="PK742_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75295.1"
                     /translation="MKRFIWLSLSLMLLGHTAKADSCDAEFVFEVTGPVVSFGSITSP
                     GPGDVLAYIWDFGDGSFGDAADPEHEYAVAGTYEVCLTVIFWDSCDASFCDDVTTLEG
                     ALDCTASFVYTIDGMSVDFEAITMPGPGDVSDYVWTFGDGGSDSDDPTPNWDYDTPGE
                     YEVCLTVYFDGGCTADFCDVIVVGGGAGGDCTALLDVWAYDGLNIHFWGGVDPDYDIV
                     FYSFDFGDGESYSETADSGGADPWHTYSEPGAYEVCLFIETGSGCTDNICIPVIVGDS
                     TGTGDCEATFIWEASGLDVDFEAVTVPGPDDVNTYVWNFGDGTIEESDDDTPNHDYAT
                     PGSYMVCLTVYFATGCEAEYCQEITVTEDGGDAECDAYFDLVSLTPDGTGWTVILNNE
                     STVTGSDIGSVIWYFGDGSIATTFDAEHYYETPGEYEICVVITSADGSCVDEYCDNII
                     CGGGTGECEADFDYETDGLTAVFENNSDGIGLAYTWTFGDGDISYETNPDHTYDAPGT
                     YLVCLTVYGADSCTDTFCDEVEVDGIVGDCAAYFTVDDITASGEGWIVSFKNESEGDY
                     IGQVWTFGDGDGSITSDPEHYYAESGTYIVCLVIGDSALGCFDTYCQEIWVGGIEDCI
                     DESVMDTTIECTAVYEPVCGCDGITYDNECYAYYYGGVLFWTEGPCLSDGVEEEVLLG
                     MKLAPNPAGDISGIYLHTNVNGPVQIRVFDLAGQLRMEYSIRSTASDYFSFDTSELPG
                     GMYLIQVLADGKSTTDKLLINR"
     gene            complement(8203..9585)
                     /locus_tag="PK742_01525"
     CDS             complement(8203..9585)
                     /locus_tag="PK742_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75296.1"
                     /translation="MTRIKHWHTFTLLAGLLAWNGTAAAQVATMPDTAGICTGFEKTF
                     NVLTNDSGDELELTAILIGPAFGSATFTPDGNITLFYADGEEFDPTDAIIYAAQDTSG
                     EIGIGYFTILVMNGDDCVWPGDANDDALVNNADVLPLGLFFEDNGPVRPDLSVEWTAQ
                     YSIDWEYPSLLEITDRKHADTNGDGVINNDDLSPIYDNYGEEHAKGTAMEGGDGFPPL
                     GIDFYADTIFSGAEVNIPLYLGTEDIPASNIYGLAFTLYFDNDLIVPGSMSVTFNTGW
                     LSSDTGMLISFNREDHPNGLFDVAVSRTNKTSATGYGHIGTVSFVMEDNLAGKITGGT
                     TMVFNMCPDDPTISNALGGYSYAEALDLTCDSVLVLDLTASVANLNSRGWSVYPNPVT
                     TNLFIQTENSPGITYQIFDPSGRMLQAGPVIHDKQPILLSDIAPGTYLLVLTDAEGMQ
                     YTQTIIKIND"
     gene            9820..11229
                     /locus_tag="PK742_01530"
     CDS             9820..11229
                     /locus_tag="PK742_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75297.1"
                     /translation="MLKSKLLEFMSHMDQKQLQRFGEFLASPYFVKDDKLYLFFQAIR
                     KYAPEFDSAKLEKSAFVKKGVEGLHLDEKKLSYLMSDLTEAGERFLKAELLMQKDLEG
                     YCALLSTYNDWESDKLYEQTLRKARKHLEESQYRNPDFFYQQYLLQSELNAYFDRQKK
                     RALDMSLQQAANYLDLYYLSVKLRYSCELINRQKLVAADYDLRMLREVRSHIEEHDYT
                     EFPSIMIYYRVLMTFLENDDTGHFDSLKALLAEHANAFPPEEARDLYAYAQNYCIRKA
                     NAGKESFLRELLQLYQASIEEGLVLTDGHISPWSYKNIVSVATRVQETDWAEQFAKQY
                     KKHLHEKFRNNAFNYNMAYLLFARKQFGKALTALLQVEFTDVFYALDSRTLQIKIYFE
                     LEETDALMSALEAFKVYLRRNRTISDSVRTIYMNFVRFVNRILSVPPSDSEKKQKLHE
                     KILEAGNVADIRWLLQKTA"
     gene            complement(11272..12183)
                     /locus_tag="PK742_01535"
     CDS             complement(11272..12183)
                     /locus_tag="PK742_01535"
                     /inference="COORDINATES: protein motif:HMM:NF036911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HQU75298.1"
                     /translation="MNYRKLLFLLAAPVLILSSCSKNIQEQINYEDFINQDDYRLMQV
                     AYYTETQDGKSLAVRFYESPRIFDLRKDLSQFDEIKKQLDISKDEKTPVQVYASDNIV
                     STVRKASENDMRIFAEWHQPVIETRSTLDSTINSIDELMSIWDFIVKQSCDSPTVSID
                     QCISFQYVVDGCYARAHKMKQILEDNYGYALEKCFSFEDASGYLAVDAGDCCVYWWYH
                     VAPYVKVQTPKGILSCVLDPSMFNKPVTKEKWFKFQENTYCSPGAKWGYYEIQPSSWY
                     GPGGSTDPYYTWTNYTLNAYSDLVTCP"
     gene            12264..12692
                     /locus_tag="PK742_01540"
     CDS             12264..12692
                     /locus_tag="PK742_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75299.1"
                     /translation="MKPVYALFLIVMTCAAVQAQQDILPVLLKMEQAMESGSYRKMLK
                     LTDGAYREEQLENMLQGNLQQFIDELISGYSQPDQTGAYLSVKLSDINKVSLCSVTDK
                     SPGKEVVFRMQLSDGRIVWTVMLFVEDPETRKWGAVGAYG"
     gene            complement(12689..13462)
                     /locus_tag="PK742_01545"
     CDS             complement(12689..13462)
                     /locus_tag="PK742_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU75300.1"
                     /translation="MIEISHIHKVFNGVTVLNDVSAVFESGKTNLIIGISGSGKTVLM
                     KCIVGLLQPDSGQVLFNKESFHEMPPERRKEVRRQIGMLFQGAALFDSLTVEENVMLP
                     LNMFSHSSKKEKLERVNFCLERVNLAGANHKFPGEISGGMKKRVGIARAIVLNPKYLF
                     CDEPNSGLDPKTSLVIDELIIELTKEFNITTIINTHDMNSVMEIGENIIFLHKGQKAW
                     EGNSHQIIESDNQELNSFIFAGEFLREARDHLISQRKKL"
     gene            complement(13459..14199)
                     /locus_tag="PK742_01550"
     CDS             complement(13459..14199)
                     /locus_tag="PK742_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU75301.1"
                     /translation="MRFVRDLGLYMKMLGRMFSRPERWNMYWKELLRQMNDIGVGSLS
                     IVLMASLFIGAVTAVQSAYQLYGTPFVPPYYLGLIVRNTTILELAPTFTCLILAGKVG
                     SNIASELGTMRISEQIDALEIMGVPTETYLVMPRVIASVIVVPIMVIIAASAGVFGAW
                     IAANFTGYGSQEEFVYGLRAFFDPFDVQIMLIKSFSFGFLISTISCFKGFYVRGGALE
                     IGKASTQAVVLSCIYVLLADYVIAEVLL"
     gene            complement(14235..15326)
                     /locus_tag="PK742_01555"
     CDS             complement(14235..15326)
                     /locus_tag="PK742_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQU75302.1"
                     /translation="MRWNWLLIPWWMTGHAMAQTDLPVTTDSSRYDGRLVVAPLIYYQ
                     PETSWAFGGGALYTLTDRLQPDNNPGLLKLLGVYTLENQVQSELGGDLYFEHNTWYLS
                     FNFTYYRFPGLFFGIGNENPAENAESYTVSKPYGRINLVRDVGHHISAGFKTFYEYTR
                     LVEIAEGGLLDTEDVSGETGGHNTGIGPWLVADTRNDIFYPTRGWKADLSAVWHDHLL
                     GSDFEYTEVRMEFSAFHAVWKQQVLAWNLLADLHPGNPPFNRMAMMGGQFYMRGNFEG
                     RLRDKHLLTTQMEYRVPVWRFIGVTGFGGIGEVGDALSSFTWSGLRYSYGAGLRLELM
                     PGSNLKLRVDYGFGYDGDRGLYVQFNEAF"
     gene            complement(15349..16092)
                     /locus_tag="PK742_01560"
     CDS             complement(15349..16092)
                     /locus_tag="PK742_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQU75303.1"
                     /translation="MCYDIQQMGDKIRRQAERQLGVSSVDFSQMDFHFFHVNGFARPE
                     ILAVTSDQPQLLRPLRWGLIPSWVKTAEEADRLSRQTLNARNDGVFSKPSFRSSIRTR
                     RCLIPVNGFYENRDVKGRKFPYFVHPANSEIFWLGGLFDRWTDRDSGEVIDSCSIITT
                     NANHLMSKIHNLKLRMPLIIPEDKLPIWLDPDLPDESIQQLMQPYPEKEMEAYTVSKL
                     VNRKRDHTNMEEVSMPFRYPELEYLDTQR"
     gene            complement(16092..16394)
                     /locus_tag="PK742_01565"
     CDS             complement(16092..16394)
                     /locus_tag="PK742_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75304.1"
                     /translation="MTDLIQKIMQETGLDEATARKTVDITGSYLKKRMPEVLADRMDD
                     LLKGESLGDSFKEKARQTATVTRDQLEEAFKDMSEKTSEAVDQIKDRIDRLFEKKK"
     gene            16597..18099
                     /gene="atpD"
                     /locus_tag="PK742_01570"
     CDS             16597..18099
                     /gene="atpD"
                     /locus_tag="PK742_01570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQU75305.1"
                     /translation="MPGKGKIKQIIGAVVDVSFDEGNLPEILNALTVTRENGEKLILE
                     VQQHLGEDSVRTIAMDSTDGLVRGMEVVDTGDNISMPTGDAIRGRLFNVVGESIDGIG
                     KVTSEKRAPIHRKPPLYEDLSTESEVLYTGIKVIDLIEPYAKGGKIGLFGGAGVGKTV
                     LIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESNVIKYGDEFKHSMEEGGWD
                     LSKVDESKLSESQATLVFGQMNEPPGARARVALSGLTVAEYFRDGDEKDAKGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFSHLDATTVLSRKISELGIYPAVDPLDSTSRILSPDIVGDEHYSC
                     AQRVKGILQRYKELQDIIAILGMEELSEEDKLVVHRARRVQRFLSQPFHVAEQFTGLK
                     GVLVPIEETIRGFNMIMDGEVDEYPEAAFNLVGSIDDAIEKGKKLLAEAR"
     gene            18118..18366
                     /gene="atpC"
                     /locus_tag="PK742_01575"
     CDS             18118..18366
                     /gene="atpC"
                     /locus_tag="PK742_01575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HQU75306.1"
                     /translation="MQVEILSPEKKVFSGEADSVQFPGTTGSFQVLNNHAAMIASLTN
                     GTVSVKTGKETLRFRISGGFVEVLNNVLSVLVEGAEEV"
     gene            complement(18413..20413)
                     /locus_tag="PK742_01580"
     CDS             complement(18413..20413)
                     /locus_tag="PK742_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75307.1"
                     /translation="MKWISICFILLFSSSAFSQTDSSLIKKLDSAERMLLLGETESAQ
                     AITQSVSKEIEESKDSSNLGRWLLIKGYTSRQEGNIPSAIEQYHKARKIYLQINDSVG
                     YASSIIKLAEVYYDNNQLDSSNLYCLESYQIARTYNNLYLKMVSLYYLGTLYYFLGDY
                     ETADHYLKLGVEDPDIHNTKSTVSLYAECNLLDIYASNLGELGNYNRAREYYNKEDSV
                     ARLLNDSSFIMLVRYGIGYIDLYEGKPKSGIDNCRAALDFYLSKRDIYYAEGCLECIG
                     RGHYLAGNLDSAEYYLKEAEQSALAHQFYDFKKDIYKLMSELYEKKGNYNKALSYFKL
                     FKEQDDSLKELASQSNILIVESRFRYEQLRSTANTLEEYARRDSAIINSQKKTNLFIT
                     LALSLVIILSGLLYYLYHINRNRNRILEQEVATRTAELTETNESLMLANAELEEFAHI
                     SSHDLKEPIRNINSFSSLIERRISSITNEELLDYLSIIRFNAGQMQLLVNAVYEFVHI
                     NKEDILVGIVNVSEVVEDVRRVLQSTIQDCSGEVVLKDHPETVISNRGMLFLILRNLL
                     ENGLKYNRSDRPRVEISVECVGNVDHWEIRDNGLGIAPQYHQRIFELFKRLHNREEFQ
                     GSGLGLSITRKVVNRLGGEIGIISEEGHGSVFWFRLPANNRT"
     gene            complement(20482..21651)
                     /locus_tag="PK742_01585"
     CDS             complement(20482..21651)
                     /locus_tag="PK742_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75308.1"
                     /translation="MKKQLYLIFIPALFLFGCKAAEKEFRQGDYDQAIDISVKKLQRN
                     PDKEAYILVLEEAFRRANDRDLAYINTLHMEGQPDRWDNVYNVYQGISRRQNKVAPLL
                     PLSIESEYRDAEFLFVDVVGELIAAKKNAASYFYAHAQQLLATGDRYDARDAYYELQQ
                     ISKFYNDYQDADKLMAEARAAGMSRVGFQVVNNSDQVLNRNLVDAMEALAPVATQGMW
                     YNIYPSDKGDDLTVQLRINRLQAFPEQVHTNSYTDMKEVEDGWTYLYDDEGHVVKDST
                     GNAIKVPKYETITALISENWQEKVASIEAELRYLDQRGNVLKSVPLKGDGVFQNYFAT
                     ATGYYDAISPESRQKLGGGPAPFPSNEALLLQAMGVLEGLMHNAMRDWNDDLLNR"
BASE COUNT         5907 a         5056 c         5079 g         5844 t
ORIGIN      
        1 atgttatcag tactggtgta acctactctg ctctgataag cagtgctact gacaaagact
       61 ggtataagtt taccaccagc gcacccaata ccaagatcaa ggttacactg accagcctgc
      121 cggctgatta tgatgttcgt ctatatcgtg gcaatagcca gaaaggtata tctgagaatg
      181 caggtacagc tgatgaagta atcatttgga ataccaattc tcagggtgca cgctatgtct
      241 atgtgtacgg atgggatggc gcatttgacg ctgctgattg ttatgacctg ctggtggaag
      301 caagcagctc ttcctggaga acagatggct cagaaaccat tatagacgaa gaatggaaca
      361 acgctatagt tggtatcttc ccgaaccctg caagctcagt tgctactgtc aactacttca
      421 gcatccagga gaatactcag gtgtttgtta ccgtaacaga tattttgggc aataaagtga
      481 tgacctctca ggcttcagtt accagcggcg ataatcttat tgagctgcag gtacaggatc
      541 tggcttcagg catctacctg gtagatgtgt ctaacggcaa gtctcactat acagagaagt
      601 tcgttaaaga ataagctggt tcagatcaga tatgaaaagg gtggccttga gccgcccttt
      661 ttttttaggc catacctgat cacacagacc tgatgatgcc tgatacctgt accggtcata
      721 ctgatctcac gcagaacgtg cattctttgt taattttgaa ccatgcagga tgtattcgac
      781 aaagtagcgg aagtcagaaa acagatcgaa gaagcagcaa tcggaagcag cgtagaactg
      841 gaggctttcc gtgtcagatt tcttggttca aagaatatcc tgaaagacct gttttcggcc
      901 atcaaggaag ttcccaatga acgaaagaaa gagtttgggc agctgatcaa tgaggtaaaa
      961 agtctggctg agtcagtttt tcaggaaaag agcgtccttt ttgccgacac aaataaacag
     1021 tctgccaggc agattgacct gtcgctgcct gctgttacca ggaaccaggg tgccatgcac
     1081 cccatcacgc tagtacggga agaaatagtt catattcttg agcgcattgg ttatgtagta
     1141 gcagaggggc cggagatcga agatgactgg cacaatttta cagcactggc catgcctgat
     1201 aaccatcctg caagagatat gcaggatacc ttttacattc agaccaaccc tgatattctg
     1261 ttaagaactc atacaagtaa tgtgcagatc agggtcatgg aaaaacagga gcctccaatc
     1321 aggatcatcg cacccggaag aacctatcgc aatgaaacca tctctgccag agctcattgc
     1381 atctttcacc aggtggaact gctgtatata gatgagcacg tttcattcgc tgatctgaaa
     1441 cagacattgt tatatctggc acaggaactt tttggatcac agacaaagat caggcttcgg
     1501 ccaagctact tcccgtttac agaaccatca gctgaactgg atgtgagctg ttttatctgt
     1561 aaccagcagg gatgtaatat ttgcaagcat acaggttggg tagagatagg tggtgccggt
     1621 atggtggatc cggccgtact cgaggcctgt agcatagatc ctgataagta ctctggattt
     1681 gcattcggca tggggctgga aaggatggct atgctgaaat ggcagatcaa tgacatccgc
     1741 ctgttcagtg aaaacgacct ccggttcctg cagcagttcg aaggtcgctg ataaggaacc
     1801 agtcttgcaa caagaccgtt tcaaatgaag cggaccaagc cccatacaaa acctgcaaaa
     1861 tgccctttca cctaagagat tccatccgat acatgtccag gatgactccc agacgggtag
     1921 ccaatatggt aaaggtcatc agttcatact attactctaa atatacaaaa aaaacagttc
     1981 aatggggtgt tcccatcagc ataagtattg aacctactac ctcctgtaac cttcgttgcc
     2041 ccgagtgtcc aagtggtttg cgatcattta ccaggccaac cggaatgctg aaaagcgatt
     2101 tcttcagaca gaccattgat gcattgcata aagacctgac ctatcttatt ttttattttc
     2161 agggagaacc atatctcaat ccggaatttc tggaaatggt gaagtatgct gcaggtaagc
     2221 gcatttatac agcaacttct accaatgcac attatcttac tgaagagaat gcaagaaaaa
     2281 ccgttgaaag tggactggac agattgatca tcagtattga tggtactacc caggaagtat
     2341 atgaacaata cagggtaggg gggaagctgg agaaagttct ggagggaaca cgacagatca
     2401 tcaggatgaa gaaagaaatg aagagtagca ctcctcacgt catttttcag ttccttgtgg
     2461 tacggccgaa cgaacatcag ataggcgatg taaaaaaact cgcaaaagaa ttgggcgtag
     2521 atgaggtcag actgaaaact gcgcagatct atgattttga agatggcagt gacctgattc
     2581 cgctccagac caaatacagc cgttacagga aacttgcaac cggtaagtgg gagatcaaga
     2641 atccactgaa cgaccattgc tggaaattat ggcattcctg tgtggtcacc tgggatggtt
     2701 tggttgttcc ttgctgcttt gataaagatg cacagcaccg gcttggtgat cttaaactga
     2761 ctccgttccg tgagatctgg caggcatggc cctatcagga attcagagct tctgtgctga
     2821 aaggaagaag ccagatcgat atctgcagaa actgcactga aggactaagt gtctggaaag
     2881 aatgattcaa tgactgaact gcaaccatta tttcttcgcc atctggcaca aacaagtgat
     2941 gaaccgctgt tgctggagat cacccacgct aaaggctgtg tattattcgg acctcaagaa
     3001 aggtcatggc tggatctgat atccggaatt tcagttagta atatggggca tggccatcct
     3061 gacattgtac aggccgtcca ggaacaggct gccaagtata tgcatgtcat ggtgtatggt
     3121 gagtttgtgg agtctccaca ggtacagtat gctacatggt tggccaacca cctgccgggt
     3181 gagatcgact cggtctattt tgtaaacagc ggaagtgaag ctgtagaagg agcaattaag
     3241 ctggcaaagc gtgccacagg cagaacggag atcgtatcct ttcagcacgg ttatcacgga
     3301 tctacagccg cagcccttgt cactggtgga aatgaagaac gaaaacgtgc attcagacct
     3361 ctgcccccag acaatctgat acttcctttt aatgatataa actcgatagg tcagatcagc
     3421 caccgaacgg ctgccgtgct ggttgaactg atccagggtg aagccggtat ccgaccagcc
     3481 gatgaggcat tcatacaaga acttgcttat cgttgccgtg tggttggtgc cttgcttatt
     3541 gtagatgagt gccagaccgg gtttggcaga acaggaaaga tgttcgcagc agaacactat
     3601 gatctgaaac cagatatcat gatattagga aaagcactgg gtggaggtat gcccctgggt
     3661 gcattcgccg ccggtaagga tctgatgcaa aagctcacca ctcagccagt gctgggtcat
     3721 atcactacct tcggtggcca tccggtttgc tgcgcagcag gcctggcttc tgcatcgctg
     3781 atcacaactg aattgttgca gcaggtggaa gaaaaaggaa aattcctgga agaaaaactg
     3841 catcatactg cgattaaaga aattcgcaga cgaggtctca tgatggttgt ggaccttccc
     3901 gatgaaccat tcaacaaagc ggtcatcagg cgtatgataa caaaaggtgt ggtgactgat
     3961 tggttcctgt ttcatgacaa tgggttgcgc attgcaccac cactggtaat aaccgtgccg
     4021 gagctggaat ttgctgcaag attgttattg gaatctttag atgaagttca gaaagaactg
     4081 cattcggtaa attaacttta cctgttacct tgagatcatt ttccatcata ctattgaccg
     4141 tactgagcct gcaggctagt tctgctgggg caccctgggg gttctggggg catcagcgca
     4201 tcaaccggat ggccctctac accctgccgg aagaactgtt tgccttttat aagccacacg
     4261 ctgattatgt ggaagagcat gctgttgatc ctgataaaag acgctatgca gttgtggagg
     4321 aagcaccccg gcattatata gatatagatc actatggtgt tcaccctttt gaaggcctgc
     4381 ctcacaaatg gtcagaagca gtggagaaat attcagaaga cactttacag gcttatggta
     4441 tcgtaccctg gcatgtagag ttgatgatgt acagactgga agatgcaatg cgtgagcagg
     4501 atgccaaaaa gatcatcagg cttactgcag agatcggtca ctatattgcc gatgcacatg
     4561 tacccttgca ttgtacggaa aattataacg gacaacttac aggccagaac ggtattcatg
     4621 gtttctggga atcacgcata ccggaactgc tgggcgaaga ctacgattat tttgtgggaa
     4681 aggctaccta tattccattt gtgcgagaca agatatggga tattgtgttg accagccacc
     4741 tcatgagtga tacggtatta catatggagg ccatgctgga taaagagttt cctgccgata
     4801 gaaaatatac ctatgaaacc cgcgggcaat caactgtaag aacctactct aaagagtatt
     4861 ctatagccta taatgcaagc attaacaaca tggtggagcg gaggatgcgt gagaccatta
     4921 taaacctggg aagcttctgg tacacagctt ggatcaatgc aggtaaaccg gatctgaccg
     4981 gattggaatg gacaccttat accgatgatg aactccgtga catggaagca atggataaag
     5041 cctaccgttc tggcggaggt atgaaggggc gatctgaaga atgatcaggc tctcactgtc
     5101 tgacgggatg taaccctctg taagatccag ctgagtgtaa aggaaggaat gaaatccgtt
     5161 ccgggaagag cttcttctgt aaatgtaagg aatgccccta tccatcccct tctgctgcca
     5221 aaaagatgca ggtaaatggc tgccgcaacc ggggcccata tgatgtcagt ccattcgcca
     5281 agtacaggaa tgctgtagct gaaatatcca aacagatcca gcaataaaca aacgatcaaa
     5341 ggtggtctct tttccatact atgtaacatt caaaacgtat gccgaactga aattgatata
     5401 aaaagggccg ggattttccc ggccctgcag acagtacccc ccagtactgt cctgatgatt
     5461 acagtttttc gaatatcaga tcgtctgtgc cgccgtttcc gccacttacg tcctgaagct
     5521 cgattttagt atcagttctg ctgatgatgt cccaatcgtc actcaggtct tcaaagtcat
     5581 caggagaagc aaaaaatatg ttgaaatcag gatttgacgg gctgtcatca tcgctgttat
     5641 cagtcacact ccaggtaccc gtaacagtgc ttgctccatt ggaggcggtc agtgtgccat
     5701 cttctccgaa ggtgaattca tacccattga aatgggcggt ctcctctttg tctgtatcat
     5761 agaagtaggt gatcttccag acaccgctgg taactgtaga ttctacttca gtggtagtaa
     5821 catcaggtgt gtcactggtg caccctgtca gtcctgcaag gagcagtaag gcagaaaggc
     5881 tgataattga actgctattc attttgtttg ttttatctgt tgatcaaaag tttgtctgtt
     5941 gtactctttc catcagcgag tacctggatc agatacatgc cacccggcag ctcactggta
     6001 tcaaatgaga aataatcgct tgcggtgctg cggatgctgt attccatccg gagttgacct
     6061 gcaagatcga acaccctgat ctgcacgggc ccgttcacat tggtatgcag atagataccg
     6121 ctgatatcgc cggccggatt aggagccagt ttcatgccca gcaatacttc ctcttccaca
     6181 ccatcagata aacacggacc ttctgtccag aagagcacac caccataata ataggcgtaa
     6241 cattcattgt cataggtaat gccatcgcaa ccacatactg gttcatatac agcggtgcat
     6301 tctatggtag tatccattac agattcgtca atgcaatctt ctatgccacc cacccagatc
     6361 tcctggcaat aggtgtcaaa acatccgaga gcagaatcgc caataacaag gcaaacgatg
     6421 tatgtacctg attctgcata atagtgttcc ggatctgagg tgatgcttcc gtcgccgtcg
     6481 ccgaatgtcc agacctggcc aatgtaatct ccttcagatt catttttaaa agaaacgatc
     6541 cagccttctc cgcttgctgt gatgtcatca actgtaaagt aagcagcaca gtcgccaaca
     6601 ataccatcta cttccacttc atcacaaaaa gtatcggtgc agctgtcagc tccatatacg
     6661 gtcagacaaa ccagataggt tccgggtgca tcgtaagtat gatcaggatt cgtttcatag
     6721 ctgatgtcgc catcgccgaa cgtccaggta tatgccagcc cgattccatc ggagttgttt
     6781 tcaaacacgg cggtcagtcc gtctgtttca tagtcgaagt cggcttcaca ttcgccggtg
     6841 ccgcctccgc aaatgatatt atcacagtac tcatcaacac aacttccatc agcagaggtt
     6901 atgaccacac agatctcata ttcaccgggt gtttcgtaat agtgttctgc atcaaaagtg
     6961 gtggcaatag agccgtcacc aaagtaccag atcacagagc ctatatcgct gcctgttacg
     7021 gttgattcgt tgttcagtat tactgtccat ccggttccgt caggtgtcag ggagaccaga
     7081 tcgaaataag catcacattc tgcatctccg ccatcttccg tgacggtgat ctcctggcag
     7141 tattctgctt cgcagccggt agcaaaatag actgtgagac aaaccatata ggagccgggt
     7201 gtggcatagt catgattcgg agtatcgtca tcgctttctt ctatggtgcc atcaccaaaa
     7261 ttccagacat aggtgttgac atcatcaggg ccgggaactg ttacggcttc aaaatcgacg
     7321 tcaagtccgc tggcctccca gataaaagtt gcttcgcaat cgccggtacc tgttgagtca
     7381 ccaacaatta cagggatgca aatattatct gtacaaccag acccggtttc aatgaagagg
     7441 cacacttcat aggcgcctgg ttcgctgtag gtatgccagg ggtctgcacc accactgtcg
     7501 gcggtttcag aatagctttc gccatcgcca aagtcaaatg aataaaatac gatatcataa
     7561 tcaggatcaa caccacccca gaaatgaata ttcaatccat cgtaggccca gacatcgagc
     7621 agggcagtgc agtctccgcc ggcacctcct cccactacaa tgacatcgca gaagtcggcg
     7681 gtacatccgc catcaaagta aacggtaagg catacctcat attcgcccgg agtgtcgtaa
     7741 tcccagttgg gtgttgggtc atcgctatcg gagccgccat cgccaaaggt ccagacatag
     7801 tctgacacat ctccggggcc cggcatggta atggcttcaa aatctacgct cattccgtca
     7861 atagtataga caaaggaagc ggtgcagtcc aaagcgcctt ccagagtggt tacgtcatcg
     7921 cagaaagatg catcacagct atcccagaag atcaccgtaa ggcacacctc gtaagttccg
     7981 gcaactgcgt attcatgttc gggatcggcg gcatcaccaa aagaaccgtc gccaaaatcc
     8041 cagatgtagg ccaatacatc tcccggtccg ggtgaagtta tcgatccgaa gctgaccact
     8101 ggtccggtca cttcaaatac gaactctgca tcgcaggaat ctgccttggc cgtatgacca
     8161 agcagcatta aggagagtga aagccagata aagcgtttca tgttaatcgt tgattttgat
     8221 aattgtttgt gtgtattgca tgccttcagc atcggtcagc accagcaggt aggtacctgg
     8281 agcgatgtct gagagcagaa tgggttgttt gtcatgtata acaggtcctg cctgcagcat
     8341 tcttcctgat gggtcgaaaa tctgataagt gattcccgga gaattttcag tctgaatgaa
     8401 taggttggtt gtaacgggat tgggataaac gctccagccg cggctattca ggtttgctac
     8461 ggaagcggtc agatccaaaa ccaggacaga gtcgcaggtc aggtcaagag cttctgcgta
     8521 gctgtatcct cccagtgcat tggagatggt tgggtcatcc gggcacatat taaagaccat
     8581 ggtagtgcct cctgtgatct tacctgccag gttatcttcc attacgaatg aaactgttcc
     8641 aatatgaccg tacccggtag cagatgtttt gttggtgcgt gatacagcca catcgaacaa
     8701 accattggga tggtcttctc tgttaaagga aatgagcatt ccggtatctg atgacaacca
     8761 gcctgtattg aatgtcacag acatggatcc cggtacgatc agatcattat caaagtagag
     8821 ggtgaaagca agtccatata tgttgctggc aggaatatct tcagtaccca ggtacaaagg
     8881 aatgtttacc tctgctccag aaaagatggt atcagcgtag aagtcaatac ccaggggagg
     8941 gaaaccatcg ccgccttcca tggcagttcc tttggcgtgt tcttctccat agttatcata
     9001 tatgggcgag aggtcatcat tgttgatcac cccgtcacca ttggtatcgg catgcttgcg
     9061 gtcagttatt tctagcaggc tcggatactc ccagtctatg ctgtattgag cggtccattc
     9121 aacagaaagg tctggcctta cagggccgtt atcttcaaag aataaaccca atggcagtac
     9181 atctgcatta ttgacaaggg cgtcatcatt ggcatcgccc ggccaaacac aatcatctcc
     9241 gttcatcacc agaatggtga agtatccaat gcctatctca ccggaagtgt cctgcgctgc
     9301 ataaataatg gcatcggttg ggtcgaattc ttctccatct gcatagaaca gggttatatt
     9361 gccgtcagga gtgaaagtag ctgaaccaaa tgccggcccg atcaggatgg cagtcagttc
     9421 gagttcatcg cctgaatcat tggtcagcac attgaaagtt ttttcaaaac ctgtacaaat
     9481 gccggctgta tcgggcatgg ttgctacctg tgcggcagcc gtaccattcc acgccagcaa
     9541 ccctgctagg agggtgaatg tgtgccaatg cttgattctg gtcatgtgct tgagctggtt
     9601 tggtaatgta aaggaaagca agttcctgag ccggcaaaag aacagatttt atctatttcc
     9661 accgttttcc gatggttttt ttgaccgtta taaggatttt tttccagaac atttgattta
     9721 agttaaagaa tatcaattga ttatgtgatt ttcaatttca tgtattctgg ggcattttcc
     9781 ggtttttagt agaaaaaatg tcaatttcgc ctcagcatca tgttaaagag taaactgctg
     9841 gagttcatgt ctcacatgga ccagaaacaa ttacagagat ttggtgagtt ccttgcctca
     9901 ccatattttg tgaaagacga taaactttat ttgttttttc aggccatcag aaagtatgct
     9961 ccggaatttg attctgctaa actcgaaaaa tcagctttcg taaaaaaggg agttgagggt
    10021 ctccatctgg acgagaaaaa gctgagctat ctgatgagtg atctgacaga agcaggagag
    10081 agattcctga aggcagaact cttaatgcag aaagatcttg aagggtactg tgccctgctg
    10141 tcaacctata atgactggga gagtgataag ctgtatgagc agactcttcg taaggccaga
    10201 aagcacctgg aagaatcaca gtacaggaat ccggattttt tctaccagca gtacctgctg
    10261 caaagtgaat tgaatgccta ctttgatcgg cagaaaaaaa gagctcttga tatgagtctg
    10321 caacaggcag ccaactacct ggatctctat tatctgtcgg taaagcttcg gtattcctgt
    10381 gaactgatca atcgtcagaa gctggtagcc gctgattatg atctgagaat gcttcgggaa
    10441 gtgcgcagcc acattgaaga acatgattat acggaattcc cttccatcat gatctattac
    10501 agggtgctca tgacctttct ggaaaatgat gataccggac attttgattc tctcaaggca
    10561 ctgctggctg aacatgccaa tgcattccct ccggaagaag caagagatct gtatgcctat
    10621 gcacagaatt actgtattcg aaaagcaaat gcaggcaagg aatcttttct gcgggagctg
    10681 ttgcaattat accaggccag tatagaagaa gggcttgtat taacagatgg tcatatttcg
    10741 ccctggtctt acaagaacat cgtttcagtt gctaccaggg tccaggaaac cgattgggct
    10801 gagcaatttg caaagcagta caaaaaacac ctgcatgaga aattcaggaa caatgcattc
    10861 aattataata tggcatacct gctatttgcc agaaagcaat ttggcaaagc acttactgct
    10921 ttgctgcagg ttgaattcac agatgtattt tatgcactgg acagccgcac actgcagatc
    10981 aagatctatt ttgagttgga agaaactgat gcactgatga gtgctctgga agcatttaaa
    11041 gtgtacctcc ggcgcaaccg taccatcagc gactcggtgc gtaccattta catgaatttt
    11101 gtcaggtttg taaaccggat attatcagta cctccatctg attcagaaaa gaaacagaag
    11161 ctgcatgaaa agatcctgga agcaggtaat gtagcagaca ttcgctggct gctgcaaaag
    11221 acagcataaa aaaaggcggt gtggaaccac cttttatttt catgtcaggt tttacggaca
    11281 ggtaaccagg tcactgtaag cgttcagcgt gtagttggtc caggtgtagt aagggtctgt
    11341 ggatccgccg ggtccatacc agctggatgg ctggatctca taatagcccc actttgctcc
    11401 cggagagcaa taggtgtttt cctgaaattt gaaccacttt tcttttgtga ccggcttgtt
    11461 gaacatgctc ggatccagta cacaactcag tatgcctttc ggcgtctgaa cctttacata
    11521 tggtgcaaca tggtaccacc agtaaacaca gcaatcgcct gcatcaactg ccagataacc
    11581 actggcatcc tcaaagctga agcatttttc cagcgcatag ccataattat cttccaggat
    11641 ctgcttcatt ttatgtgcac gtgcataaca tccgtctacc acatactgaa agcttataca
    11701 ctgatcaata ctgaccgtag gtgaatcaca gctttgcttt acaatgaaat cccagatact
    11761 catcaactca tctatagagt tgattgttga gtcaagcgta gaacgggttt caataacagg
    11821 ttgatgccat tctgcaaaaa ttcgcatatc attttcactg gctttcctta cagtggaaac
    11881 tatgttgtca ctggcataca cctgtaccgg tgttttctca tccttgctga tatccagttg
    11941 tttttttatt tcatcaaatt gtgaaaggtc ttttctcaga tcaaatattc ttgggctttc
    12001 ataaaagcga actgcaagtg atttaccatc ttgtgtttca gtgtaataag caacctgcat
    12061 caagcggtaa tcatcctgat tgatgaagtc ttcataattg atctgttcct gtatattctt
    12121 gctgcaggaa ctcaggataa ggacaggcgc agccaacaaa aagaggagtt ttcggtaatt
    12181 cattgggttt gattttgaat tttctaaatt acaggttttt tttgatcctg tagcgaattt
    12241 ttgtcaaatg acatctggtt gacatgaaac ctgtatatgc tctttttctg atcgtaatga
    12301 cttgtgcagc agtgcaggct cagcaagata tcctgccggt cttattaaaa atggaacagg
    12361 caatggaatc cggttcttac cggaagatgc tgaaacttac ggatggcgca tacagagaag
    12421 aacaactgga aaacatgctt cagggtaatc tgcaacagtt tattgatgaa ttgatatccg
    12481 gttattcgca acccgatcag acaggggctt atctttctgt gaagttgtct gacattaaca
    12541 aggtttcgct ctgctcagtg accgataaaa gcccgggaaa agaagtggtt ttccgtatgc
    12601 aattgtcaga cggcagaatt gtctggacgg tcatgttatt tgtggaggat cctgaaacca
    12661 ggaaatgggg tgctgtgggg gcttatggct aaagcttttt tcgctgtgaa ataaggtgat
    12721 cgcgggcctc gcgaaggaat tctccagcaa aaatgaaact attcagttcc tggttatcag
    12781 attcaatgat ctgatggctg ttgccttccc atgccttttg tcctttgtgc aggaagatga
    12841 tattctctcc aatttccatc acagaattca tatcatgtgt gttgataatg gttgtgatat
    12901 tgaattcttt ggtcagttca atgatcagtt catcaatgac cagtgatgtt ttaggatcca
    12961 gtccggagtt gggctcatcg caaaaaagat acttgggatt aagcacgatc gcccttgcaa
    13021 tgcctactct ttttttcata ccgccactga tctcgcccgg aaatttatgg ttggctccgg
    13081 ccaggtttac ccgctccagg cagaaattga ctctttccag tttttctttt ttgctggaat
    13141 gactgaacat attcagaggt aacatcacat tttcctcaac cgtaagagaa tcaaataatg
    13201 ccgctccctg aaaaagcatg cctatctgcc ttctcacttc ttttcgtcgc tcgggaggca
    13261 tttcatggaa agactccttg ttgaacaaaa cctgaccgct gtctggttgc aacaacccta
    13321 caatacactt catcagtacc gtttttccgc tgccggaaat acctatgatc aggttggtct
    13381 ttcctgattc aaatacagct gatacatcgt tgagtactgt aactccgttg aagaccttat
    13441 gtatatggct gatctcgatc ataacaatac ctccgcaatg acatagtcgg ccagaagtac
    13501 gtaaatacaa ctcagcacta cagcctgggt gctggccttg ccgatctcca gtgcaccacc
    13561 ccttacataa aaccccttga aacaggatat ggtagaaata agaaaaccaa agctgaatga
    13621 tttgattagc atgatctgca catcaaacgg atcaaaaaag gccctcaatc cgtacacgaa
    13681 ttcttcttgt gagccgtaac ccgtaaagtt ggcagctatc catgctccga atacaccggc
    13741 actggcagca atgatgacca tgatgggcac aacgatcaca gatgcgatga cccttggcat
    13801 gaccagatag gtttcagtag gtacacccat gatctccagc gcatcgatct gttctgagat
    13861 gcgcatggtg ccaagttctg aagcgatatt ggagcctacc tttcctgcca gtatcagaca
    13921 ggtaaatgtg ggggccagtt ccagaatagt agtgttccgt acaatgagac ccaggtaata
    13981 gggtggtaca aatggtgtgc cgtataactg gtatgcactc tgaacagctg tgacggcacc
    14041 aatgaacaat gaagccatta gtacaataga cagtgaacca accccaatgt cattcatctg
    14101 acgaagcagt tccttccagt acatgttcca cctttctggc cgggagaaca tcctgcccag
    14161 catcttcatg tacaaaccga gatctctcac aaaccgcata gcactgtggt aaaattacac
    14221 atttgcagct cgcctcagaa cgcttcattg aactgcacat acaaacccct gtcgccatcg
    14281 tatccgaaac cataatctac tctcagtttc aggttgctgc ctggcatgag ttccagtcgc
    14341 agaccggcac cataagagta acgcaatcca gaccaggtga aagatgacag agcatctccc
    14401 acctctccga ttccgccaaa accggttacc ccgataaaac gccatacggg aacacgatat
    14461 tccatctgag tggtcagcag atgcttgtct ctcaggcgcc cttcaaaatt gccacgcatg
    14521 tagaactgtc cgcccatcat ggccattctg ttgaacggag ggtttcctgg atggagatca
    14581 gccagcagat tccaggccag aacctgttgt ttccatacag catgaaatgc actgaattcc
    14641 atccggacct cagtatattc gaaatcactg cccagcaaat ggtcatgcca tactgctgat
    14701 aagtctgctt tccacccacg ggtagggtaa aagatatcat tgcgggtgtc agcaaccagc
    14761 caggggccta tgcctgtatt atgtccgccg gtttcacccg aaacgtcttc tgtatccagc
    14821 aacccacctt ctgcgatctc taccagccgg gtatattcat aaaatgtttt gaatcctgca
    14881 gaaatatgat gtccgacatc gcgaaccagg ttgatcctgc catatggttt gctgacggta
    14941 taagattctg cgttttctgc agggttctca tttccaatgc caaagaataa cccgggaaac
    15001 cggtaataag taaaattgaa agacagatac catgtattgt gctcaaaata gagatcacca
    15061 cccagttcac tctggacctg attttccagt gtatataccc caagcaattt taataatccc
    15121 ggattattat ccggttgcaa cctgtcggtt agtgtgtata gtgcacctcc gccaaatgcc
    15181 cagctggttt caggctgata gtaaatgagg ggtgctacta ccaggcggcc atcataacgg
    15241 gaagagtcag ttgttaccgg caaatcagtc tgagccatgg catgacctgt catccaccag
    15301 ggtatcagca accagttcca ccgcatctga cgaaggtact gcaacttgtc agcgctgtgt
    15361 atccagatat tccaactcgg gataacggaa tggcatgctg acctcttcca tgttggtgtg
    15421 gtctcttttt cggttcacca gtttgctcac ggtataggct tccatttctt tttccggata
    15481 cggttgcatg agctgctgaa tggactcatc tggcaggtcc ggatccagcc agataggtag
    15541 tttgtcttct ggaatgatga gtggcatgcg aagtttcagg ttatggatct tgctcatcag
    15601 gtggtttgca ttggtagtga tgatggagca ggagtcgatc acctcaccgc tgtcccggtc
    15661 tgtccagcga tcaaataaac ctccaagcca aaagatttct gaattggcag gatggacgaa
    15721 ataagggaat ttccggccct tgacatcacg attctcataa aaaccgttca caggaataag
    15781 gcaacggcgt gtgcggatgg atgaacggaa ggaaggtttg ctgaagaccc cgtcgttgcg
    15841 tgcattaagt gtttgcctgc taagcctgtc agcctcttcg gccgttttta cccaggaggg
    15901 aatgagcccc cagcgcaaag gccggagcaa ttgcggctga tcactggtta ctgccagtat
    15961 ctctggtctg gcgaagccgt tcacatggaa gaaatgaaaa tccatctggc tgaaatcaac
    16021 cgaagaaacg cccagttgac gttcggcctg gcgacggatc ttatcaccca tctgctgaat
    16081 gtcgtagcac attatttctt cttctcaaaa agcctgtcta tgcggtcttt gatctgatca
    16141 actgcttcgg aggtcttttc actcatatct ttaaaggctt cctccagctg gtcccgggtt
    16201 acagtagctg tttgtctggc tttttcctta aaactgtctc ccagagattc gcccttaagc
    16261 agatcatcca tcctgtctgc cagcacttcc ggcatcctct ttttgagata agatccggta
    16321 atatctacgg tctttcttgc cgtagcctca tccaggccgg tctcctgcat gatcttttgg
    16381 atcaggtctg tcatattatg gtgttctttc tgctaaaatt aacgaacacg gcttgttttt
    16441 tgtttcagta aagacccggc atcaggcggt gaaatgacag ccaggaggct ccggaagctg
    16501 ttttcaacaa tttcttcacg gccttgaaat atcggtatca ggtccgtacc tttgcgggcg
    16561 attttaatcg catcccagac tataaacata tttctgatgc ccggaaaagg taagatcaaa
    16621 cagatcatcg gtgctgtggt agacgtcagc ttcgatgaag ggaacctccc cgaaattctc
    16681 aacgcattga ccgtaacccg tgaaaacgga gaaaagctga tcctggaagt gcagcagcac
    16741 ctgggtgaag acagtgtccg cacgatcgcc atggactcga ccgatggtct tgttcgcggc
    16801 atggaagtgg ttgacaccgg cgataacatc agcatgccca ccggcgacgc tattcgtggt
    16861 cgcctgttca atgtagttgg agaaagtatt gatggtatcg gtaaagtaac cagcgaaaag
    16921 cgggctccta ttcaccgtaa accaccatta tacgaagatc tgagtactga atctgaggtg
    16981 ctctatactg gtatcaaggt cattgacctg atcgagccct acgccaaagg tggtaagatc
    17041 ggactcttcg gcggtgcggg tgtaggtaag accgtactga tcatggagct gatcaacaac
    17101 atcgcaaagg gatactctgg tatgtcagtg tttgccggtg taggtgagcg caccagagaa
    17161 ggtaatgacc tgctccgcga gatgatcgaa tcaaatgtca tcaagtacgg cgatgagttc
    17221 aagcacagca tggaagaagg tggctgggac ctgtcaaaag tggacgaatc caaactgtca
    17281 gaaagccagg caacgctggt gttcggacag atgaacgaac ctcccggagc acgtgcacgt
    17341 gtagcattga gcggtcttac cgttgctgaa tatttccgcg atggagatga gaaagatgcc
    17401 aaaggccgtg atatcctgtt cttcattgat aatattttcc gattcaccca ggcaggttct
    17461 gaagtgtccg cactgctggg acgtatgcca tcagccgtgg gttaccagcc tactctggct
    17521 acagagatgg gtctgatgca ggaaaggatc acctctacca agcgtggttc cattacttct
    17581 gttcaggcag tttacgtgcc tgcagatgac ctgaccgacc ctgcacctgc aactacgttc
    17641 tctcacctgg atgccaccac tgtacttagc cgtaagatct cagaacttgg tatctatcct
    17701 gcggttgacc cgcttgattc tacctccagg atcttgtcgc ctgatattgt tggagatgaa
    17761 cattactcct gcgcccagcg tgtgaaagga atcctgcaac gatataagga actgcaggat
    17821 atcattgcca ttctcggtat ggaagaattg agtgaagaag acaaactcgt ggtacaccgt
    17881 gcccgccggg tgcagcgttt cctttctcag cctttccatg ttgcagaaca gttcactggt
    17941 ttgaaaggag ttctggttcc gatcgaagaa accatccgcg gattcaatat gatcatggat
    18001 ggtgaagtgg atgagtatcc ggaagccgct ttcaacctgg ttggttctat tgatgacgcc
    18061 attgaaaaag gtaaaaaact gctggctgaa gccagataat ctttttaatt cagacagatg
    18121 caggtagaga tcctttctcc ggaaaagaaa gtgttcagcg gcgaagctga ttcagtacag
    18181 tttccgggca caacgggttc ctttcaggtg ttgaataacc acgctgccat gatcgcttcg
    18241 ctgaccaacg gaacggtcag tgtgaaaacc ggtaaggaaa cacttcgttt tcgaatttca
    18301 ggaggctttg tggaagtact caataatgta cttagcgtat tggtagaagg agctgaagaa
    18361 gtctgatctg caccagatat tggaaaaagc catccccgga ggatggcttt ttttatgtcc
    18421 tattattagc aggaagacgg aaccagaata cagaaccatg cccttcctca gatatgatgc
    18481 cgatctcacc ccccagtctg ttcactacct ttcttgtgat ggaaagacca agaccgcttc
    18541 cctgaaactc ctccctgtta tgtaatcgct taaacaactc gaatatgcgc tgatggtatt
    18601 gtggagcaat gcctaatccg ttgtctctga tctcccagtg atctacattc ccgacgcact
    18661 ctacagagat ctccacccgc ggacgatccg accggttata ttttaacccg ttctccagca
    18721 gattcctgag tataagaaac agcatgccgc gattggaaat aacggtttcc gggtgatcct
    18781 ttaataccac ctctccactg caatcctgaa tggtgctttg cagtaccctt ctgacgtctt
    18841 caacaacctc agatacattg actattccaa ccagaatatc ttccttgttg atgtgtacaa
    18901 actcgtacac cgcattcact aacagttgca tctgacctgc attgaagcga atgatggaca
    18961 gataatcaag tagttcttca ttagtaatgc tgctaatcct cctttctatc agactgctga
    19021 atgaattaat gtttctgata ggctccttca gatcatgcga agaaatatgc gcaaattctt
    19081 ccagttcagc attggcaagc atcaaggatt cattggtctc ggtaagctct gctgttcggg
    19141 tcgcaacctc ttgttccagg atccggtttc tgtttcggtt tatatgatag agataataca
    19201 acagaccgga aagtatgatc accagactca aggccagggt gatgaacagg ttggtctttt
    19261 tttgactgtt tattatggcg ctgtcccgcc gggcatactc ctccagagtg ttggcagttg
    19321 atcgcagctg ttcataccgg aacctggatt caactataag aatattgctt tggctggcca
    19381 gctctttcaa tgaatcatcc tgctctttga agagtttgaa atagctgaga gctttgttgt
    19441 aattgccctt tttctcatat aactcagaca tcagtttata gatgtctttc ttgaaatcat
    19501 agaactgatg agctagtgct gattgttctg cttctttcag ataatattct gcagaatcaa
    19561 gatttcctgc caggtaatgc cccctgccta tacactccag acaaccttca gcataatata
    19621 tatcacgttt ggagagataa aaatcaagag ctgcacgaca attatcaata ccacttttgg
    19681 gtttgccttc atagaggtct atataaccta tcccgtagcg aacaagcata ataaaagagc
    19741 tgtcatttag aagccgggct acagaatctt ccttgttata atactcccgg gctctgttat
    19801 agttgcctaa ttcacccagg ttggatgcat agatgtccag cagattgcat tcggcatata
    19861 gactgacagt ggacttggtg ttatggatgt cagggtcttc cacacctaat ttcagataat
    19921 gatcggcggt ttcataatca ccaagaaaat aatacaaggt gcccaggtag tacaggctga
    19981 ccatcttgag gtacagatta ttataggtcc tggctatctg ataggattcc aggcaataaa
    20041 ggttggaact gtccagctga ttgttatcat agtaaacttc tgccagcttg atgatggatg
    20101 acgcatagcc aacagagtca ttgatctgaa gatagatttt tcttgctttg tgatattgct
    20161 caatggctga cggaatgttg ccctcctgcc tggatgtgta ccccttgatg agtaaccatc
    20221 tgcccaggtt tgaactgtct ttgctttctt caatttcttt tgagacagat tgggtgatag
    20281 cctgagctga ctctgtttca ccgagtaata acatgcgttc tgcagagtcc agtttcttga
    20341 tcaatgaact gtcggtctgc gaaaatgcag aagaactaaa aagtaaaatg aagcatatac
    20401 taatccactt catacaacag ctaaccttgt aatgcagagc tgaatacgaa ggtacgatat
    20461 gtgtccttat gtgatgttgt gttatctgtt cagcagatcg tcgttccagt cacgcatggc
    20521 attgtgcatc aagccttcca gaactcccat ggcctgtaac aacaaggctt cattgctggg
    20581 aaagggggca ggtccgccac ccagcttttg acgagattcg ggcgaaatgg catcatagta
    20641 tcctgtggcc gtagcaaaat aattctggaa tactccgtca cctttcagcg gaacagattt
    20701 cagtacattg ccacgctgat ccagatatct caattctgcc tcaatgctgg caactttttc
    20761 ctgccagttc tctgagataa gtgcagtgat ggtttcatat tttggtactt ttatagcgtt
    20821 gccggtactg tctttcacta catgcccttc gtcatcatat agataggtcc aaccgtcttc
    20881 tacttccttc atatcggtat agctgttggt atgcacctgt tcaggaaacg cctgcaacct
    20941 gttgatgcga agctgaacag tcagatcatc ccctttatcg gaaggatata tattgtacca
    21001 catgccctgg gtggccacgg gtgcaagggc ctccattgca tctacaagat ttctgttcag
    21061 aacctgatca ctgttgttta ccacctgaaa tccaactcgg ctcattccgg cagctctggc
    21121 ctctgccatc agcttgtctg catcctggta atcattgtag aatttgctga tctgctgtag
    21181 ttcataatag gcatcccggg catcataccg gtctccggtt gccagtaact gctgagcatg
    21241 cgcatagaaa tagctggctg cattcttctt ggcggctatc agttctccca ccacatcaac
    21301 aaacaggaat tcggcatccc ggtattcaga ttcaatagaa aggggaagca agggcgccac
    21361 cttattttgt cgccggctga taccctgata tacgttgtaa acattgtccc agcggtcagg
    21421 ctgtccttcc atatggaggg tgttgatata cgccagatcg cggtcattag cccttctgaa
    21481 ggcttcttcc agcacaagta tgtaggcctc cttatcgggg ttgcgctgca gttttttaac
    21541 agatatatct atggcctggt catagtcacc ctggcgaaac tctttttcag ctgctttgca
    21601 gccaaacagg aaaagtgctg gaataaatat caggtaaagc tgcttcttca tatgttctgc
    21661 tttcacaaag gtattcctaa aactatgcca tactgccgta tctcagctgg ttataaaagg
    21721 aaaatacaga cacttgttcc taaggccata gttatttaga aaatgtctaa ataaaaaccc
    21781 gtgagcacct gaaaatactg atgtttcaga atcccgacat caggtgttga aaaccctata
    21841 attttctcaa ggtttgttaa agattttgaa acacctgatg tagatt
//
LOCUS       DAOMAX010000012        21088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_259_length_21088_cov_3.889222, whole genome shotgun sequence.
ACCESSION   DAOMAX010000012 DAOMAX010000000
VERSION     DAOMAX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21088
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_21088_cov_3.889222"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..979
                     /locus_tag="PK742_01590"
     CDS             113..979
                     /locus_tag="PK742_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75309.1"
                     /translation="MKALLPLVFVLSGSLLFSQSAYRFDDNKVWLEESGCRVTQGDLD
                     HYRFLTEFVLGAPISSAQRSLLRSQVMDACTDRTAVYMQGRQAAQALLKQCRMSSNPA
                     QIGLLSSIQAYAYLSQPEDPDKALVLSWMKESNPVLATDTTLGLVFRLADAYACLNLI
                     RLNHFMLQDSVPVVTNESWQLAIDSLSRQYLMTGQSDRKYLSSMQFVWVQTAMAWQTL
                     DIGSRQEISRIFREERHGKYRNGMQDLQLTEDISRALIEAAGITPDQVIQALGSGRSD
                     LFGWRKLDTLQW"
     gene            998..1405
                     /locus_tag="PK742_01595"
     CDS             998..1405
                     /locus_tag="PK742_01595"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HQU75310.1"
                     /translation="MDFSNVNFLAVIVSGIAAWVIGALWYSPLLFSKRWQKETGLSDD
                     DVRQANMAVIFGGSLVLMIIMALGLAMHIQTGQADLMRGLKSGLLTGVFFSATIIGIN
                     YLYQRKSITLWLIDAGYAVLFLGVEGAILGVWQ"
     gene            1551..1832
                     /locus_tag="PK742_01600"
     CDS             1551..1832
                     /locus_tag="PK742_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQU75311.1"
                     /translation="MNIFVAKLSYNTTDDTLRSAFERYGEVSSAKIIIDREMNRSKGF
                     GFVEMPNDDEANNAINGLNETEMDGRTIVVKEAQPRENNRGGGGYNRRF"
     gene            1978..3084
                     /locus_tag="PK742_01605"
     CDS             1978..3084
                     /locus_tag="PK742_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQU75312.1"
                     /translation="MAFGINKRRIRKALRRLQSLPGGWNFFLYSGNKIRTWWLRTIKS
                     PKVAHPSSIMLEVTNQCNLRCITCPREYQYGEEMAKGFMNLDALKKVVDEAYPYVDSI
                     GLTGLGETLMYKQLPEALDYIRSKNKGIITTISINAHLPGTVEMVKKIADKLDTLQIS
                     MDGVGQVYEDVRLRSDYDFFYNNVKEIVAWCKDKRAEVMFNFVAVKENYHTMAEVIEV
                     ASELGVVDVNITPFNVAAVTAHGIDYYDFFHTEAFKQELLRAKEKADQLKNVTMTVWD
                     IKSPNEFQKCHLPWGHFYISWDGYATPCCAKPFPKELNFGNVFDDGLMHCLNTPGYLE
                     FREMWYKNEVPSFCEKCHMVDLKPIDLPIELPVN"
     gene            complement(3081..3299)
                     /locus_tag="PK742_01610"
     CDS             complement(3081..3299)
                     /locus_tag="PK742_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75313.1"
                     /translation="MRTETLVLIKDILIILFFLAGTLWTWGIWKLGKERQERMEFIFR
                     NSRNRFKWLFLIGLIILIAARIFRMTAG"
     gene            3353..4240
                     /locus_tag="PK742_01615"
     CDS             3353..4240
                     /locus_tag="PK742_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75314.1"
                     /translation="MHRILSCTLALLTTTIAFAQEWNLQKGEAKVFGSYSFLHYDRIA
                     GPEGTFDETGFVVDDMSFGLFAVYGVNDKWTLFLDLPYKRIEASPYIYLDDMSAPDRP
                     AYLQDVPEPDGGTLTAPGNAKAGASFALRPEELTVSLTFEANTSTQNYIDGTATGFNS
                     SAVIPGIAWIHGTNKWWSSVLVQGEWRTNNYSSVLAGRAEAGLHLTNWLYVAAQPAWR
                     IPLNESDADCDCTLAYTALYLNEQSYVSMQLKTGVEYRNWGLHLASGNAFYVRNVGLN
                     LALTGGLSYHFTADRSSNE"
     gene            complement(4207..4800)
                     /gene="coaE"
                     /locus_tag="PK742_01620"
     CDS             complement(4207..4800)
                     /gene="coaE"
                     /locus_tag="PK742_01620"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQU75315.1"
                     /translation="MKIGVTGNIGSGKSTVCKLFAVLGIPVYHADERAKWLLENDPNI
                     IAQVEDLLGKEAYSNGRLNRKLIAEKVFADADLLKRYNAVIHPAVQEDAMRWEEAHAS
                     APYTIREAALLVESGSYKALDMLITVTAPEALRIARVMQRDGVGREAVEARVRNQMPE
                     EKKVALSQAVIINDGEQQLIPQVWKIHHSLLERSAVK"
     gene            complement(4797..5480)
                     /locus_tag="PK742_01625"
     CDS             complement(4797..5480)
                     /locus_tag="PK742_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HQU75316.1"
                     /translation="MRANPDYARFRPGESTLRYLVCLLLAAAMWLFLAMNRQYDSHVR
                     AGLEIQLPDDVWVSDSIAEHLELKVHATGWQLLRMAVNPPALMISESSITGRSVLDMK
                     SESSVIIPQLPDQVELLDVYPAFVPLHTVSKESKRVPLKLETESAPGWAIDSLVIRPD
                     SVTLTGPSSRLSEIDHWSTELVVLPADSGWVLGKAALLPPGELEASAIVVNYHVLVHQ
                     GSDNSTEEP"
     gene            complement(5490..5831)
                     /gene="yajC"
                     /locus_tag="PK742_01630"
     CDS             complement(5490..5831)
                     /gene="yajC"
                     /locus_tag="PK742_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQU75317.1"
                     /translation="MYNAVLLMADPNGQSSDGSGFTTIGFFLLFFVILYFFMIRPQTK
                     KAKDQKNFLGELKAGDKIVTIGGVHARIAKVEDETLLVEVDNNVKLRIEKSVISLDYT
                     RAAQNRKSQQS"
     gene            complement(5849..6319)
                     /locus_tag="PK742_01635"
     CDS             complement(5849..6319)
                     /locus_tag="PK742_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQU75318.1"
                     /translation="MKKMLYIPLMAALALGFMSCNESKENSDGSLPTDLIHNPQSAEG
                     EVDMENLPIMTFEKDLHDFGEIVQGEKVTYEFKFTNTGKSDLLITSARASCGCTVPDY
                     PKEPIAAGQTAYIKVQYNSEGRKDAFNKTVTITANTIPNENKIRIKGVVIVPQN"
     gene            complement(6335..7279)
                     /gene="nusB"
                     /locus_tag="PK742_01640"
     CDS             complement(6335..7279)
                     /gene="nusB"
                     /locus_tag="PK742_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQU75319.1"
                     /translation="MLTRRGLRVKVMQVLYMVSQDRETGQQTASRLLKENIRNTYRSL
                     IYLLHFLTRLAEQVQAEADRRKAKYIPSEEDLTFNTILYTNPVTEYLRNSRFLKDQMR
                     KERLVSGDEEELVEAVYAELKAWDVYSQYQTQTSYTTEDHNRFLRALVRKFLPANEAF
                     DQFMEDMIPTWADEEQVVLAEADTLLRVFPLSAEREATLFHADLPLEEQEFADTLLNS
                     CLAEEERFNGLIEGRLQNWELERVSLLDRILMKMALTEFLHLPTVPVKVSINEYLEIS
                     KLYSTPKSKEFINGVLDKLMIELKASGAIIKTGRGLIE"
     gene            complement(7289..8386)
                     /locus_tag="PK742_01645"
     CDS             complement(7289..8386)
                     /locus_tag="PK742_01645"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQU75320.1"
                     /translation="MSHQSLLSAEGDIYQMLSDMGHEQLVFCSDEKTGLKAIIGIHNT
                     VLGPALGGTRFWSYASEKDAIVDVLRLSRGMTFKSAISGLNLGGGKAVIIGNSDKLKN
                     REPLFRRYGKFVESLNGKYITAEDVGTSTSDIEFIGMETGHVAGKPEQLGGGGDPSPV
                     TAYGTYLGMKAARKFRDGTDSLEGQRVMVQGAGHVGFYLVERLVKEGAKVMVSDINND
                     RLKKLTDAFQVEVVAADAVYDQQMDIYAPCALGATVNDDTISKLSCGIIAGAANNQLK
                     EEHKHGEALVQRNITYAPDFLINAGGVINCYVELEGYNRERAYQKAEIIYQRTIDILE
                     LAAAEDLTPQAAAIRMAQERIQAVAAMNAVM"
     gene            8477..10258
                     /locus_tag="PK742_01650"
     CDS             8477..10258
                     /locus_tag="PK742_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU75321.1"
                     /translation="MRALAAVNRYFLKYRWLLLLGTLFVILSNIFKALSPGVIGDALD
                     LLIRQLQTYQQLTDEAAKAELRSDVLSGTGRLTLYYLALALGNGLFLFLMRQTLIIMS
                     RHIEYDQKNELFAHYQKLTPAFYKRHNTGDLMNRVAEDVGRVRMYVGPALMYFLNTIT
                     LFILVIWAMLKVNPRLTFFVLLPLPVLSVSIYFVNNLIEKRSERIQRQLSKLTSVAQE
                     IFSGIRVIKAYAQEEENSRYFEEQTDIYKDRSLSLARVDAMFQPLMVLLIGLSTLLVV
                     FVGGFEVIQGRLTAGNVAAFIIYVNMLTWPVASLGWVVSIVQRAAVSQRRINEFLQTQ
                     PDISSTIPDQGIISGNLEFSHVTFTYPDSGIRALEDISFHIKAGQRVAIIGRTGSGKT
                     TIADLLLRLYEPDSGKIRLGGRPVQDYSLHDLRASIGYVPQDVFLFGDSIQNNIRFGL
                     PDEQQARVEDYAKAAGIHDEIDSFRQGYDTLVGERGVTLSGGQKQRISIARALIKDAP
                     LVVLDDCLSAVDAATEQQILDHLTGMLEKKTALIITHRLSAMEQYDQIILLNEHHIAA
                     TGTHAELLRTSAEYREMTEASRKGELI"
     gene            10318..10797
                     /locus_tag="PK742_01655"
     CDS             10318..10797
                     /locus_tag="PK742_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HQU75322.1"
                     /translation="MDNDNRNRSENVFSRKIRAGRRRTYFFDVRTTKGNDYYLTITES
                     KKRQGGDGYERHKIFIYKEDFNKFLEEMTAAVDYIKERMPDFDFDSFSHKYDDDDDYG
                     DQEVREPREEYRKEAPAAEEVKAEDAEPMEDPPASEPSEEDLPPRQDPTINEDDLKW"
     gene            complement(10798..11457)
                     /locus_tag="PK742_01660"
     CDS             complement(10798..11457)
                     /locus_tag="PK742_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQU75323.1"
                     /translation="MPLIYSNQPYPDTKAGVWRIEEPPEFFLKHLIFSNWERQFYNGL
                     KHPERQMSWLSSRYLLKLLLDTREYVELLFDTNGKPFLGSGSHRISLSHTHKYAAAII
                     SESEEVGVDVEDAGRDIGHLSRKFLSDAERRFLGEQPPNEELLLYWGAKEVIYKMYGR
                     RKLDFRQDMFIRPFQVADKGVMAGVLLKNGNMTSYSLPYLRGDGFSLVTGIGTDSKPI
                     S"
     gene            11534..11752
                     /locus_tag="PK742_01665"
     CDS             11534..11752
                     /locus_tag="PK742_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75324.1"
                     /translation="MRLFQFIHREEDSLQISIPENLRGKYVRITVEECAPEDGEPISD
                     LENSGLKQKEEALLLLDKLRNQLDVWDI"
     gene            complement(11753..12115)
                     /locus_tag="PK742_01670"
     CDS             complement(11753..12115)
                     /locus_tag="PK742_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75325.1"
                     /translation="MSSLFTAFLLSAGIMSNAAAVSEPVPATINDQMQIVLPADQPLA
                     AVYAFSIADLKFTEAAQAEQFFSMFTENVVVYVVDFESRTVQVYLQDIMTPAWDITAW
                     NDYFAARSMKMKVVYDAL"
     gene            complement(12150..12617)
                     /locus_tag="PK742_01675"
     CDS             complement(12150..12617)
                     /locus_tag="PK742_01675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQU75326.1"
                     /translation="MYSFTTMTPHQQALLHMFHQAPVNKFFQHITLRFELDQAAVELP
                     ILPEYMHAGGSLHGALYMKLLDDAAYFSAALLESSRFLLTAGFALDFYRPVHGGVLTA
                     TGKVKGQEGKSILAASTLTDEDGNLVARGHGRFVPGNGLLLETPLYISGLPSN"
     gene            complement(12596..14761)
                     /locus_tag="PK742_01680"
     CDS             complement(12596..14761)
                     /locus_tag="PK742_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75327.1"
                     /translation="MNRKWWLIWVLMVSVTGRSQYVLYDVPVINPFTDASMQFPFCGG
                     FNNPQFSDVDMNLDGLTDLLIFDRSGNVAMVLYRQSFPGEPPQFSYQTSLDPFLPVMR
                     DWALTYDYNCDDLPDLLTYISGSAALYRGVITDGHLDFQLEVPELLYTSSGGFILPVY
                     TSRTDLPGLADVDGDGDMDILAFSLSGVQIRWYRNNSVESGYGCDSLIYSIADYCWGE
                     IFEGATCDGADLGVECLGEGSEEQSRMHVGSTILVFDKDLDGVQDMVLGDVSCDNLVY
                     YQNGGTPGFASMVSKDTLYPVPSYPAKLPVFPGAYLVDMNADGGKDLLVAPNDDLYSV
                     NNRHILWYSQITPGGPQELAFTDRDFFTGNAIDMGDYSHPAWMDVDGDGLMDMVCGVR
                     ENKALEELPSTGLWYWRNTGTAEDPTFALVDDDLFGLQELGILSLSPSAGDADGDGDI
                     DLMLGAADGTLIYMENTAGPGAAVAMNEPVLFYEGIDVISYSKPCLFDVDLDGAMDLI
                     IGNVNGVLHYYHKEEGSYVLVSSGWGGVDVRRPGDLVGHSAPFMFRNESGLMQLVVGS
                     ASGHIFLYDEIEESMLGEFHERDTLFLGILPGMFSTISGTDLNNDGEPEFAVGNIRGG
                     LHLMQRDPDVAIGQQTEPEREILIYPNPASEQLLVQGLRPGNWTYHLLDMNGRLQMSG
                     TLAPDYRIRGIHVLMPGCYILELSEEGGKLLRQCIVLQP"
     gene            complement(14758..16920)
                     /locus_tag="PK742_01685"
     CDS             complement(14758..16920)
                     /locus_tag="PK742_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU75328.1"
                     /translation="MGRSSRWLLVFVLFSPGLMAQSYVLDPYATVTEHSRTLLYPFAG
                     GLNNPQFWNIQLDNDGLTDLLVFDRNGGKVLTFRNTGTNWVYAPEYEYEFPAMEHFVV
                     TADFNCDGLEDLFTSFDDGVSVYKAVPEGSNISYVLETEKLEYEEIGFSFDVGVGIID
                     IPAFVDVNGDGDLDMLTFKLIGGFVDYFENQAVENGQPCTLQLEHVNSCWGDFYESGI
                     TKAVQLDSSCSETPVDKSMLHGMHAGSTFLVFDEDADGDMDLVLGDLSFQNLNKLVNG
                     GDASLAHIVEQDTVFPGYDIPVDMTTFPAPFLLDVDLDGRQDLLVSPNKEGFSINTHN
                     VSYYRNVSVDDTFVFQFQEDSLFVSDMVDVGSEAHATFFDHNNDGLLDIVIGNYGYFN
                     AGTFNGKLALYENTGTADAPSWTLMTRNYADLEIYGFRGLHPTFGDINGDGKTDMLVG
                     EENGFVHYFKNIAADGADATFILAGPNFQGIDVGKYSTPQLVDVNGDELLDLIIGEEN
                     GNLNYYENTGTATDPVFTLLDELWGSVDVRKPLTLTGYSTPWLTYDADGNAMLLVGCL
                     AGSIFQYTPTPDFSGAFTKVTEDYAAIDDGDRTTVCTADLDGDGILEILTGNDRGGIT
                     LYRDASTIVQTNEVFVPGKLHPFPQPAADVMFLSVSDQELTHLFIYDLTGKLVECVAL
                     GQERPLRISLEHIMPGMYVLNAADARGWVHYTGKLIRQ"
     gene            complement(16988..19444)
                     /locus_tag="PK742_01690"
     CDS             complement(16988..19444)
                     /locus_tag="PK742_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQU75329.1"
                     /translation="MSSTQHIPRSYSLPGIFLLLFLSACSNARYLQEGEVLYKESKVT
                     FSDSTRIPDELKGLDNDLARLSKLQPNKKVLGIYPLKMWLYNLGNRGFDQYLDYAAAR
                     KGSLSLAPWLALSPPLQPDSELREWLTVKVGEAPSLIDTGLIYDTGIRMENYLYNRGY
                     FYPQSQFSVEVDERRHRATVHYEVTPNKLYRIRSVVYELDDPELKRLALLDSASSPLK
                     QGRRFDVDFLKAERKRIADDLSDNGYLYFNKEYVYFQVDTTVADANIDVFVKVSPPEN
                     DSIHHRYKIRDIYILSDMSGPVNDTIRFDEKTRKGEFRSAFYILQDHERYTPKSLVDN
                     VFINQGDYYTQSKVSQTIGAFSNLGMFKYATISTTPIDTSGEIRSMDLTIKLEPLPKH
                     DITTEFNTSTTSEYLLANYGSFSYTRLNTFRRLDALRFSVDGGIESQITNRNVALNTS
                     EFTTQVNLLFPRFFWPFSLYTPKNYYPRTITTLKLEYLDRVQFYTLFNTAFKYGSERF
                     EGSRKQQLNLYPIDVNYVRVPRSTAQFDSILENNFLLRQAFQEQLILAPNATYILNTQ
                     AVADSRNDLYFRAGGELAGSLFFIGTAIKNGAPLLGSESQLTDPFRIFDIPFSNYVRL
                     DLDFRDYFDINTGNTLVGRAAFGIAVPYWNSQVVPFVKQFYVGGANSLRAFPIRKVGP
                     GSYLSYEINDEGVATLKPEDRTGDVKIEMNAEYRFDIAGILKGALFCDVGNVWSLQED
                     ASRPGAKFQLSDFYKELAVGPGAGLRLDFSYFIFRLDAAYPLVDPAVDGPKGAYYQNE
                     LGITFPDKTINWNFAIGYPF"
     gene            19427..20152
                     /locus_tag="PK742_01695"
     CDS             19427..20152
                     /locus_tag="PK742_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQU75330.1"
                     /translation="MLSAAQIKHLTSLQLKKYRQKYAEFVIEGGKILAEALQEGVEVK
                     AVIATPAQLDKCRLLAGNIPVLEADEKQMKNISSMSEPPGILAWCSMRKASEMQQPDG
                     WQLALDGIRDPGNLGTIIRTADWFGVRNILLSEDCADIYNPKTLQSSMGSMFRCSIIQ
                     GTLTDLLRKANKPVFAAAADGDSMDQLRTASGVLVIGSESHGISEEVKRVCDHSVAIP
                     GDGRTESLNAAVAAGILLYGMVN"
     gene            complement(20447..>21088)
                     /locus_tag="PK742_01700"
     CDS             complement(20447..>21088)
                     /locus_tag="PK742_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75331.1"
                     /translation="YPDSREFRIITWQMELKDLSHRYFGAIQMHSDSLVLFPLIDMSM
                     FIPGIDSVILDQDNWFGQLYYDVQEFKFKKERYYLLFGWDANDMWSNKKIVDVLWFDE
                     GGKPKFGKPVFEIEEGDIRDRVIIEYKEDASPAMVWDDRLNMIIFDYLQPENPLSEGI
                     YMTYIPDGTYQGFYFDKKKKHWILQRVVFDQTLDKAPINKPKQKGEDPNLYNN"
BASE COUNT         5461 a         5193 c         5127 g         5307 t
ORIGIN      
        1 cgggtagtgg agatacccct gaaacggtgg tctttcagat ctacctccgc gaccgtgcag
       61 gtcatcagag caacgtggtc tccactcccg ccatcacagt caatccgcaa taatgaaagc
      121 tttgctcccc ctggtattcg tgttgtcagg atcgctgctt ttttcacagt cagcctatcg
      181 ctttgatgac aacaaggtat ggctggaaga aagtggttgc cgcgttaccc agggcgatct
      241 ggatcattac cgttttctta cggagtttgt actgggtgct cccatcagca gtgcacaaag
      301 aagtttgctg cgcagtcagg tcatggatgc atgtaccgac agaactgcgg tatatatgca
      361 gggcagacag gctgcacagg ccttactgaa acagtgcaga atgagcagca atcctgccca
      421 gataggtctt ctcagcagca ttcaggccta tgcatatctg tcacagcctg aagaccctga
      481 caaagccctg gtattgtcat ggatgaagga aagtaatccg gtactcgcaa cagatactac
      541 cctggggctg gtttttcgtc tggcagatgc ctatgcctgc ctgaatctga tccggctcaa
      601 ccacttcatg ttgcaggatt cagttcctgt agtaaccaat gaatcctggc agctagccat
      661 agacagtctg agcagacaat atctgatgac cggacaatca gaccgcaagt atttgtcgtc
      721 catgcagttt gtctgggtgc agacagctat ggcctggcaa acgctggaca tcggttccag
      781 gcaggagata tccaggatat tccgggaaga gcggcatggg aaataccgaa acgggatgca
      841 ggacctgcag cttacagaag acatcagcag ggcattgatc gaggcagcag gtatcactcc
      901 agaccaggtc atacaggcac ttggaagcgg gcgcagtgat ttgttcgggt ggcgaaaact
      961 ggatacctta cagtggtaaa acccaactaa ccataacatg gacttttcaa acgtgaattt
     1021 tctggcggtc atcgtaagcg gtatagccgc atgggttatt ggtgcactct ggtacagccc
     1081 cttacttttc agtaaaagat ggcagaaaga aacaggcctg agcgatgacg acgtcaggca
     1141 ggccaacatg gctgtcatct ttggaggcag cctggtgctc atgatcatca tggctctggg
     1201 gctggcaatg catattcaga ccggtcaggc cgatctgatg cgagggctga aatctggact
     1261 gcttaccggg gtgttctttt cagctactat tattggcatt aattatctgt atcagcgaaa
     1321 gtcaattaca ctctggctga tcgatgccgg ctatgcggta ctgttcctgg gtgtggaggg
     1381 agccatactg ggagtatggc agtgagccgg actgcttttg gataaataaa tcagctttcc
     1441 cttttttgaa ataaataagc gtatctttga gctgtactta tactgtacaa ggcacgggtc
     1501 ggatttaacc aaacaaggcc cttgttttaa acagttatca attatttttt atgaacattt
     1561 ttgttgccaa actttcctac aacactactg atgacacact tcgcagcgcc tttgaaaggt
     1621 atggtgaagt aagttcagcc aagatcatca tcgatcgtga aatgaatcgt tccaaaggat
     1681 tcggattcgt agaaatgcct aatgatgacg aagcaaacaa cgccatcaac ggacttaacg
     1741 agaccgaaat ggacggccgc accatcgtgg tgaaggaagc acaaccccgc gaaaacaacc
     1801 gcggtggtgg tggttacaac cgtcgtttct aacagtttcc aaggatcgta agaaaccccc
     1861 tacagcccat ccgggcacac tgccgtgatc aagttatcgc ggaggctgta catcaacatg
     1921 tgcaaccgat ccaaaggatc agtattcgga tgggctgttt tgtatttttg tgcagccatg
     1981 gcattcggta tcaacaaacg caggatcaga aaagctctcc gaaggcttca gagtcttccc
     2041 ggaggatgga atttctttct ctattccggt aataagatcc gtacctggtg gttgcgcacc
     2101 atcaaaagtc cgaaagttgc tcacccctct tccatcatgc tggaggtaac caaccagtgt
     2161 aacctgcgct gtatcacctg ccccagagaa taccaatacg gagaagaaat ggccaagggt
     2221 ttcatgaacc ttgatgccct gaaaaaagta gtggatgaag cttaccctta tgtagattcc
     2281 atcggcctta ccggactggg cgagacactc atgtacaagc agctgcctga agcactggac
     2341 tatatacgct ctaagaacaa aggcatcatc acaaccattt ccatcaacgc ccacctgccg
     2401 ggcactgttg agatggtcaa aaagatcgct gataagctgg ataccctgca gatctccatg
     2461 gatggtgtgg gtcaggtcta tgaagatgtg cggctgcgca gtgattacga tttcttctac
     2521 aacaatgtga aagagatcgt agcctggtgc aaagacaaac gggccgaggt catgttcaac
     2581 tttgtggcgg tcaaagagaa ctatcatacc atggctgaag tgatagaagt agcttcagaa
     2641 ctgggtgtgg tagatgtcaa tatcacccct ttcaacgtag cggctgttac agctcatggc
     2701 attgactact acgacttctt tcataccgaa gcattcaaac aggaactcct gcgtgcaaag
     2761 gaaaaagcag atcagctgaa gaacgtcacc atgaccgtat gggatattaa aagcccgaac
     2821 gagtttcaga aatgccacct tccgtgggga catttctata tcagctggga tggctacgct
     2881 accccctgct gtgccaaacc atttccgaaa gaactcaact tcgggaatgt ctttgacgat
     2941 ggcctgatgc attgcctgaa cactccggga tacctggagt tcagagagat gtggtataag
     3001 aatgaagtgc cctcgttctg cgagaaatgc catatggtcg atctgaagcc catcgacctg
     3061 ccgatagaat taccggtcaa ttaacctgcc gtcatccgga atatcctggc agcgatcagg
     3121 atgatcaatc cgatcagaaa caaccatttg aaacggttcc ggctgttccg gaaaatgaat
     3181 tccatccgct cctgacgctc tttccccagc ttccagatcc cccatgtcca cagcgtaccc
     3241 gccaggaaga acaggatgat caggatgtct ttgatgagta ccagtgtctc tgtgcgcata
     3301 ctgtaaagtt gcgcaaattt cagcgcatgg atgcagctat gtatatttga gaatgcaccg
     3361 gatattatca tgcacgcttg ccctgctcac cacgactatc gcatttgcac aggaatggaa
     3421 tctgcaaaag ggtgaagcga aggtcttcgg atcatatagt tttcttcatt atgacaggat
     3481 cgcaggaccg gaaggcacct ttgatgaaac aggatttgtg gtggatgata tgtccttcgg
     3541 attatttgct gtgtacgggg taaatgacaa gtggacattg tttcttgacc tcccttacaa
     3601 acgaatagaa gcttctccat atatctatct ggatgatatg tcagcacccg atcgtcctgc
     3661 ttatctgcag gatgttccgg aaccggatgg aggcacccta accgctccgg gcaatgcaaa
     3721 agcaggagca tctttcgcac tcagacctga agagctgacg gtttccctga ccttcgaagc
     3781 aaatacttcc actcagaatt atatcgatgg cactgctacc ggcttcaaca gcagtgcggt
     3841 cataccgggt attgcctgga tacacggcac caacaaatgg tggagcagtg tgctggtgca
     3901 gggtgaatgg cgcacgaata actacagttc tgtgctggca ggcagggctg aagcagggtt
     3961 acatctgacc aactggctct atgttgcagc gcaacctgcc tggagaattc cattgaatga
     4021 atctgacgcc gactgtgatt gtacccttgc gtacacggca ttgtatctca atgaacagag
     4081 ctatgtgtcc atgcagctga agaccggcgt agaatacagg aactggggct tacacctggc
     4141 ttcaggaaat gcattctatg taaggaatgt tgggttgaac ctggcactta ccggaggtct
     4201 ctcctatcac ttcactgcag atcgttccag caacgagtga tggatcttcc acacctgagg
     4261 gatcagttgc tgttcgccat cgttgatgat gacagcctga gataaggcca cctttttttc
     4321 ctccggcatc tggttgcgca ctcttgcttc cactgcctcc ctgcctaccc catctctctg
     4381 catcactctt gcaatacgca atgcttccgg tgcggtaact gtgatcagca tatccagtgc
     4441 tttgtaggaa cctgactcta ccagcaaggc agcttcacgg atggtgtaag gtgcagatgc
     4501 atgtgcttcc tcccatcgca tggcatcttc ctgcacagcg gggtggatga cggcattata
     4561 gcgcttcaaa aggtctgcat cggcgaaaac tttttctgcg atcagtttcc tgttgagcct
     4621 accgtttgaa taagcttcct tccctaaaag atcttctacc tgagctatga tatttggatc
     4681 attttccagc agccatttcg cccgttcatc agcgtggtac accggaatac ccagcacagc
     4741 gaaaagtttg cacacggttg atttgccact gccaatattt cctgtaacac cgatcttcat
     4801 ggttcttcgg ttgaattgtc agagccttga tgaaccagga catggtaatt gaccacaatg
     4861 gctgatgctt ccaactctcc cggaggcagc aatgctgctt tgcccagtac ccaaccactg
     4921 tctgccggca gcacaacgag ttcagttgac cagtgatcga tctctgaaag ccggctggaa
     4981 ggcccggtca gggtgaccga gtcgggccgg atgacaagac tatctattgc ccagccaggg
     5041 gctgattcgg tctccagctt cagaggtact cttttgctct ctttgctgac cgtatgcagc
     5101 ggcacgaagg cgggatagac atccagcaac tcaacctgat caggcagctg gggtatgatc
     5161 acagagcttt cacttttcat atccagcacg gatcttcctg tgatcgatga ttctgagatc
     5221 atgagcgcag gcggatttac agccattctg agcaattgcc atccagtggc atgaaccttt
     5281 agttccaggt gttctgcaat ggaatcagac acccacacat catcaggcag ctgaatttcc
     5341 aggccagctc tgacgtggct gtcatactga cggttcatag ccagaaaaag ccacatggct
     5401 gctgccagca agaggcagac caggtagcga agggttgatt cgccgggacg gaaacgtgcg
     5461 taatcaggat tggcacgcat cccgttccat taagattgct gagactttct gttctgcgca
     5521 gcccgggtat agtcaagaga gatgacagac ttttcaatgc gtagtttcac attgttgtca
     5581 acctcaacca ggagggtctc atcttctact ttagcaattc ttgcatgcac cccaccaatg
     5641 gtcacgatct tatcacctgc cttcagttca cccagaaagt tcttctggtc tttggccttt
     5701 ttggtttgcg gcctgatcat gaagaaatac aggataacaa agaacaagag gaagaagccg
     5761 atggtagtga acccggatcc gtctgatgac tgtccgttgg gatcggccat taataaaaca
     5821 gcattataca tatacatatc tttgagtatt aattttgcgg aacgatgacc acacctttga
     5881 tacggatctt gttctcgttc ggtatggtat tcgcggtgat ggtgacagtt ttgttgaatg
     5941 catcttttct gccttcactg ttgtactgta ctttgatgta ggcagtttgc ccggctgcta
     6001 taggttcttt cgggtaatca ggcacggtac atccacaaga tgccctggca gaagtgatga
     6061 gcaggtctga tttgccggta ttggtgaact tgaattcgta ggtcactttt tcgccctgta
     6121 cgatctcacc aaaatcatgc aggtcttttt cgaaggtcat gattggcagg ttttccatat
     6181 ccacttcgcc ttctgcgctc tgcggattat ggatgagatc agtaggaaga ctgccgtcgc
     6241 tgttttcctt tgattcatta caagacatga aacccagggc cagagcagcc atcaggggga
     6301 tgtagagcat ctttttcata cggtaaaatt aatgttattc gatcaaaccc cttccggtct
     6361 tgatgatggc accggatgct ttcagttcga tcataagctt gtcaagaaca ccattgatga
     6421 actctttgct tttaggtgta gaatacaact tgctgatctc caggtactca ttgatactga
     6481 ctttgaccgg aaccgttggc aggtgcagga attctgtcag agccattttc atcagaatcc
     6541 gatccaggag ggagaccctt tccagttccc agttttgcaa tcgcccttcg atcaatccgt
     6601 taaagcgctc ttcttctgcc aaacagctgt taaggagggt atctgcaaac tcctgttctt
     6661 ctaagggaag atcggcatga aacaaggtcg cttcccgctc agcagacagg ggaaaaaccc
     6721 ttaaaagggt atctgcttcc gccagcacca cctgttcttc atcggcccag gtgggtatca
     6781 tatcttccat gaactgatca aaggcttcat tggctggcag gaatttcctg accaaagcac
     6841 gcagaaaacg attgtgatct tcagttgtgt aggacgtctg agtttggtac tgactgtaca
     6901 catcccaggc tttcagttca gcatagacag cttcaaccag ttcttcctca tcgccggata
     6961 ccagcctttc tttgcgcatc tgatctttca gaaaacggga gtttcgcagg tattctgtga
     7021 ccggattggt ataaagtatc gtgttgaagg tcagatcttc ttcgctggga atatacttag
     7081 ccttccgccg gtcggcttca gcctgtacct gctcagccag tcgggtgaga aaatgaagca
     7141 gatagatcag cgaacgatat gtgtttcgga tgttctcttt cagcaaacgg gaggcagtct
     7201 gctgaccggt ctctctgtcc tgagaaacca tatacagcac ctgcatcacc ttcacacgca
     7261 atcctcttct agtcagcata ggttgtaatt acatcacagc attcatcgct gccacagcct
     7321 ggatgcgttc ctgagccatt ctgatggcag cagcctgagg tgtaagatct tcggcagcag
     7381 ccagttcaag aatatcgatg gtacgctggt aaatgatctc tgctttctgg tatgcacgtt
     7441 cgcggttata gccttccagc tctacataac aattgatcac tccacctgcg ttgatcagga
     7501 aatcaggtgc ataggtgatg tttctctgta ccagtgcttc tccgtgtttg tgttcttctt
     7561 tcaactggtt attggcagca cctgcgatga taccgcaact caacttggag atggtatcgt
     7621 cgttcacggt ggcgcctaaa gcgcaaggtg catagatatc catctgctgg tcatatacgg
     7681 catcggcagc aaccacttct acctggaacg catcggtcag ttttttcagc ctgtcgttgt
     7741 tgatgtcaga caccatgacc ttagctcctt ccttcacaag cctttccacg aggtaaaagc
     7801 ctacatgtcc ggcaccctgc accatcaccc tttgtccttc caggctgtct gttccgtcgc
     7861 ggaatttcct ggccgccttc atacccaggt aggtgccata agctgtaacg ggagaagggt
     7921 cgccgccacc acccagctgc tctggttttc cggccacatg tccggtctcc ataccgatga
     7981 attcaatatc tgaggtagat gtgcctacgt cttctgcagt aatgtacttg ccgttcaggc
     8041 tttccacgaa cttgccatag cggcggaaga gtggctcgcg gttcttcagt ttatctgagt
     8101 taccgatgat gaccgctttg ccgccaccga ggttaagtcc ggaaatagca gacttgaagg
     8161 tcataccacg tgaaagtcgc agcacatcaa caatggcatc tttttcagaa gcataagacc
     8221 agaaccgggt gccccccagt gccgggccca atacagtgtt gtgaatacca ataatggctt
     8281 tgagtccggt cttttcgtca gaacagaata ccagctgttc gtgacccatg tctgaaagca
     8341 tctgatagat atcgccttca gcgctgagga gggattgatg agacattatg aacgattttt
     8401 ttggcgggtc aaagatacgg gcttcctctg aagttgcgaa caaggttgtc aaagtcaaag
     8461 ccattagttt tgcacaatgc gggcactggc ggctgttaac cgatactttc ttaaataccg
     8521 atggctttta ttattgggta ccctgttcgt catcctgtcg aatattttca aggcattatc
     8581 gcccggagtc ataggagatg cactggacct gctcatcagg cagctgcaaa cgtaccagca
     8641 gctgacagat gaagctgcaa aagcagagct tcgcagtgat gtgttatccg gcaccggcag
     8701 gctgaccctg tattacctgg cactggcgct gggcaacgga ctctttttgt tcctcatgag
     8761 gcaaacgctt atcatcatga gcaggcatat cgaatacgat cagaagaacg aactgtttgc
     8821 gcattaccag aaactcaccc ctgcctttta caaacgacac aataccggcg atctgatgaa
     8881 cagggtagcg gaagatgttg gccgggtgcg catgtatgta ggtcctgccc tgatgtactt
     8941 tctaaatacc attacgcttt ttatcctggt tatctgggct atgctgaagg tcaacccaag
     9001 gcttaccttt ttcgttttgc tgccacttcc ggtactgtct gtttctattt atttcgtcaa
     9061 taatctgatc gagaaaagga gtgaacgcat tcaaaggcaa ctcagcaaac tgaccagtgt
     9121 agcgcaggag atcttttcag gcatccgggt catcaaggca tatgcccagg aagaagagaa
     9181 cagcaggtat tttgaggaac aaaccgacat ttacaaagac agaagtttat cacttgccag
     9241 ggtagatgcc atgttccagc cgctcatggt gctgctgatc gggctcagca ccctgcttgt
     9301 agtctttgtt ggcggctttg aggtcattca gggaaggcta acagcaggca atgtggcagc
     9361 gttcatcatt tacgtcaaca tgctgacctg gcctgttgcc agtcttggtt gggtggtgag
     9421 catagtgcag cgtgccgccg tttctcagcg caggatcaat gagtttttgc aaacccaacc
     9481 ggatatcagc agcaccatac ctgatcaggg tatcatttca ggcaacctgg agttttctca
     9541 tgtaaccttt acctaccccg attcaggcat cagggcgctg gaagatatca gcttccacat
     9601 caaggcgggg caaagggtag ccattatcgg ccggaccggc agcggaaaga ccactattgc
     9661 agatctgctg ctaaggctgt atgagcccga cagcggtaaa atcaggctgg gcggaagacc
     9721 tgttcaggat tacagcctgc acgatctgcg ggcttccata ggctatgttc ctcaggatgt
     9781 atttctgttc ggcgacagca tacagaacaa tatacggttt ggtttgccgg atgaacagca
     9841 agcaagggtg gaggactatg cgaaggccgc tggtattcat gatgagatag acagcttcag
     9901 gcagggttac gatacccttg ttggagaacg aggtgtaacc ttgtcaggcg gacaaaagca
     9961 gcgcatcagc atagcaaggg ctttgatcaa agatgcaccg ctggtggtgc tggatgattg
    10021 cctttctgcc gtagatgccg ctactgaaca gcagatcctg gatcatctga caggcatgct
    10081 ggagaagaag accgctctca tcattaccca tcgcctcagt gctatggagc aatatgacca
    10141 gatcatatta ctgaacgagc accatattgc ggcaaccgga acacatgccg aattgctgcg
    10201 taccagtgct gagtatcgag aaatgacaga ggcatccagg aaaggagaac ttatctgagg
    10261 catttggaaa gtcatttttt tttatctttc ggacattaac atcaaaaccc aggcgcagtg
    10321 gataacgaca acagaaaccg cagcgagaat gtattttcaa ggaaaatacg tgcaggaaga
    10381 agaagaacct atttctttga cgtgcgcacc accaaaggga acgattatta tctgaccatt
    10441 accgaaagca agaagcgtca gggtggcgat ggttatgaaa ggcataagat ctttatttac
    10501 aaggaagatt ttaacaagtt cctggaggag atgaccgcag ccgttgatta catcaaggaa
    10561 cgcatgccgg atttcgactt tgacagcttc agtcacaagt atgacgatga tgatgactat
    10621 ggcgatcagg aagttcgcga gccacgggag gaataccgca aagaagcccc tgctgctgaa
    10681 gaagtaaagg cagaggatgc agaaccaatg gaagaccccc cagcaagtga gccttctgag
    10741 gaagaccttc cccccaggca ggatcctacc atcaatgagg atgacctgaa gtggtgatca
    10801 gctgatgggt ttggaatctg ttccgatacc cgtaacaagg ctgaacccat cccctctcag
    10861 atagggcagt gaatagctgg tcatgttgcc gttcttgagc aatacccctg ccatgacccc
    10921 tttatcggcc acctgaaacg gcctgatgaa catatcctgt ctgaaatcca gttttcgcct
    10981 gccatacatt ttatagatca cttccttggc tccccagtac aacagcagtt cttcattggg
    11041 cggttgttca cctaaaaatc tcctttctgc gtcagacagg aatttccggc tgagatggcc
    11101 aatatctctg cctgcatctt ccacatcaac tcctacctct tcagattcgg aaatgatggc
    11161 agctgcatac ttgtgtgtat gcgacaaact gatgcggtgt gaccctgaac ccagaaaagg
    11221 ttttccgttg gtatcgaaca acagctccac atattcacgt gtatccagaa gaagtttgag
    11281 caggtatctg ctggacaacc agctcatttg tctttccgga tgcttcagtc cgttatagaa
    11341 ctgcctctcc cagttgctga aaataaggtg cttcaaaaag aattccggag gttcttcaat
    11401 acgccaaaca ccggctttgg tatcaggata tggctggttg gagtagatca gaggcataat
    11461 gagcgatcag caaaaataat ttggccggaa agggcacaaa cagtaatttc ggaataccct
    11521 aagttgtaac atcatgcgat tattccaatt catccaccgt gaggaggatt cgctgcagat
    11581 cagtattcct gaaaatctgc ggggaaaata tgtgcgcatc accgtagaag agtgtgcccc
    11641 tgaagatgga gaacccatca gtgatctgga gaattccggt ctgaaacaaa aagaggaggc
    11701 attgctcctc ctcgataagt tacgtaacca gctggatgtc tgggacatct gatcacaaag
    11761 catcatagac caccttcatc ttcatggaac gggcggcaaa atagtcattc catgcagtga
    11821 tatcccaggc tggtgtcata atatcctgaa gataaacctg aacggtacgg gattcaaaat
    11881 ctactacata gaccaccaca ttctctgtga acatactgaa aaactgttca gcctgtgcgg
    11941 cttccgtgaa cttcagatca gcaatactga atgcatatac tgcagcaagc ggctggtctg
    12001 ccggaagaac gatctgcatc tgatcattga tagtagccgg caccggttcg ctgacagccg
    12061 ccgcgttact catgatacct gctgagagga gaaatgctgt aaacagacta ctcattgata
    12121 agtatttata acgctaagtt accgtttccc taattgctgg gcaacccgct tatgtacaag
    12181 ggcgtttcca gcaacaatcc atttccgggc acgaacctgc catgacctct ggctaccaga
    12241 ttcccatctt catcggtcag ggttgaggct gccagaatgg acttaccctc ctgccctttt
    12301 accttacctg ttgccgtgag tacacctccg tgtacaggtc tgtaaaagtc aagggcaaaa
    12361 cctgcagtca gcagaaaacg ggaactttcc agcaaggcgg cagaaaaata ggctgcatca
    12421 tccagtaatt tcatatacaa tgcaccgtgc agcgatccgc cggcatgcat atattccgga
    12481 aggatcggta gctctaccgc cgcctgatcg agttcaaatc ttagggtgat atgctgaaag
    12541 aatttattga caggagcctg atggaacata tgcaacaaag cctgttgatg gggtgtcatg
    12601 gttgtaaaac tatacattgg cgaagaagtt tgcctccttc ttctgataat tccaggatgt
    12661 aacaaccagg catcagtaca tggatccctc tgatgcgata atcgggtgcg agggttccgc
    12721 tcatttgcaa ccgaccgttc atatccagaa gatgatacgt ccagtttccg ggtcggagac
    12781 cttgtaccag caattgctcg ctggccggat taggatagat cagtatctcc cgttcaggtt
    12841 ctgtctgctg accaatggct acatccggat ctcgttgcat cagatgcaaa ccaccgcgaa
    12901 tatttcctac agcaaactcc ggttcaccat cattattgag gtcagttcct gaaatggtgc
    12961 tgaacatacc gggcaggatt ccgaggaaga gggtgtcacg ttcatgaaat tcgcccagca
    13021 tactttcttc gatctcgtca tacaaaaaga tgtgaccact ggcagaaccc accacaagct
    13081 gcatcaggcc ggattcattg cggaacatga atggcgcact gtgccccacc agatcgccgg
    13141 gtctgcgtac atctacccca ccccatccgg agctgaccaa aacataactg ccttcttcct
    13201 tatggtagta atgcagcact ccgttcacat tgccaatgat cagatccata gctccgtcca
    13261 ggtccacatc aaacaaacaa ggcttcgaat aggaaataac atctatgcct tcatagaaca
    13321 gtaccggctc attcatggcc actgcagcac ccggaccggc ggtgttttcc atgtatatca
    13381 gtgtaccatc ggcagcaccc aacataagat cgatatcacc atcaccgtct gcatcacctg
    13441 ctgagggcga caaactcagg atacccagtt cctgcagtcc gaacagatca tcgtcaacca
    13501 gggcaaaagt cggatcttct gctgttcctg tgtttctcca gtaccaaagt ccggttgaag
    13561 ggagctcttc cagtgcttta ttctccctta caccgcaaac catatccatc agaccatcgc
    13621 catctacatc catccaggcg ggatgggaat aatcgcccat atcaatggca ttgccggtaa
    13681 aaaaatcccg gtcggtgaac gccagttcct gcgggcctcc gggtgtgatc tggctgtacc
    13741 acaggatatg ccggttgttc acactgtaga ggtcatcgtt tggtgctacc agcaggtctt
    13801 tacccccatc agcattcata tcaaccagat aggcacccgg aaagaccgga agttttgccg
    13861 gataggaagg cactggatac agcgtatctt tagaaaccat actggcaaac ccgggtgtac
    13921 ctccgttctg gtaatagacc aggttatcgc aggaaacatc gcccagcacc atgtcctgca
    13981 caccatccag gtctttatcg aacaccagta tcgtacttcc tacatgcatg cggctctgtt
    14041 cttcagaacc ttcgccgagg cattccactc cgaggtcagc accgtcgcag gtggcacctt
    14101 caaagatctc gccccagcag taatcggcaa tggaataaat aaggctatca cacccatatc
    14161 ctgattccac gctgttattt ctgtaccatc tgatctgcac cccggagagc gaaaaggcaa
    14221 gtatgtccat atcgccatcg ccatccacat cagccaggcc gggcaggtct gttctgctgg
    14281 tataaacagg caggatgaat ccaccggaag aagtgtacaa cagttcaggt acttccagtt
    14341 gaaaatcaag atgaccatcc gttatcacgc ccctgtacaa tgccgcactt cccgatatgt
    14401 aggtaagcag gtcaggcaaa tcatcacagt tgtaatcata ggtgagtgcc cagtcacgca
    14461 tgacaggcag aaaaggatca agcgatgttt ggtaactgaa ttgcggaggt tctcccggaa
    14521 acgactgcct atacaacacc atggcaacat taccgctccg gtcaaatatg agcaggtctg
    14581 tcaggccatc aaggttcatg tccacatcag agaattgcgg attattgaat ccgccgcaaa
    14641 aggggaattg catggatgca tcagtaaagg gattgataac aggtacgtca tataatacat
    14701 actgtgaacg tccggtcaca gacaccatca gtacccagat caaccaccat tttctgttca
    14761 ttgtctgatc agctttcctg tatagtgtac ccatcccctt gcatctgccg cgttcagcac
    14821 atacatgccg ggcatgatat gttccagcga tatgcgcagc ggcctttcct ggcctaatgc
    14881 aacacactcc accagttttc cggtcagatc atatatgaat aaatgtgtca gttcctgatc
    14941 agaaacagat agaaacatga catcagcagc cggttgcgga aatggatgga gcttgccagg
    15001 cacgaacact tcatttgtct gtacgatggt agatgcatcg cggtaaagtg tgatgccacc
    15061 ccggtcgtta ccggttagta tttcgagaat accatcacca tccagatcag ctgtacacac
    15121 ggtagttctg tcgccgtcat cgatagcagc ataatcttcc gtaaccttgg tgaaggcacc
    15181 tgaaaaatca ggagtaggag tatattgaaa aatacttcct gccagacacc ccaccaacag
    15241 catagcattg ccatcggcat cataggttag ccagggagtg gaatagccgg taagagtaag
    15301 aggtttacga acgtccacac ttccccacaa ttcatcaagc aaggtgaata ccggatcggt
    15361 agccgtaccg gtattctcgt agtagttgag gttgccattc tcttcgccga tgatgagatc
    15421 aaggagttca tcgccattca catccaccag ctgcggggta ctgtatttac ccacatcgat
    15481 gccctgaaag tttggcccgg ccagtataaa agtggcatca gcaccatctg cagcaatgtt
    15541 cttgaagtaa tgcacaaacc cgttctcttc accaaccagc atatctgttt taccatcgcc
    15601 gttaatatca ccaaaggtgg gatgcagccc tctgaaaccg tagatctcca ggtctgcgta
    15661 gttgcgtgtc atcaatgtcc atgacggcgc atctgccgtt ccggtatttt cgtatagtgc
    15721 cagcttccca ttgaaagtac cggcattaaa atatccgtaa ttgccgataa cgatgtcgag
    15781 caggccgtcg ttgttgtggt caaaaaaagt ggcgtgtgct tctgaaccta catcaaccat
    15841 atctgacaca aaaagggagt cttcctgaaa ctgaaagacg aaagtatcat caacagatac
    15901 atttctgtag taagagacgt tatgtgtatt gatactgaac ccttccttgt tgggtgatac
    15961 gagcaggtcc tgccttccat ccagatcaac atccaacaga aaaggcgccg ggaaggtggt
    16021 catatcaaca ggaatatcat agccgggaaa gaccgtatcc tgttccacga tatgtgcaag
    16081 ggaggcatca ccaccgttga ccagcttatt caggttctga aagctcaggt ctccgagtac
    16141 cagatccata tctccatctg catcttcatc gaacaccaga aatgtggaac cggcgtgcat
    16201 gccatgcagc atacttttgt caaccggagt ttcagagcag ctgctgtcca gctgcacagc
    16261 tttggtgatc cctgattcat aaaaatctcc ccagcaggaa ttcacatgct ccagctgcaa
    16321 ggtgcaaggc tggccgttct ctactgcctg gttctcgaaa tagtcaacaa aaccaccaat
    16381 cagtttaaag gtcagcatat cgaggtcgcc atctccgttc acgtccacaa aagccggaat
    16441 atcaataatg cccacaccca catcaaagct gaagcctatt tcttcatatt ccagcttttc
    16501 agtttccagc acatagctga tattactgcc ttccggaact gctttgtaca cggatacgcc
    16561 atcgtcaaaa gaagtaaaga gatcttccag gccatcgcag ttgaaatctg ctgtaaccac
    16621 aaagtgttcc atggccggaa attcatattc atattccgga gcatagaccc agttggtacc
    16681 tgtattgcgg aaagtaagca ctttccctcc gttccggtca aaaaccaaca ggtctgtcaa
    16741 cccgtcgttg tccagttgaa tattccagaa ctgaggattg ttcaaaccgc cagcaaacgg
    16801 gtacaataaa gtgcggctgt gttcggtcac tgtagcgtac ggatcaagga cataagattg
    16861 cgccattaat cccggcgaaa acaagacgaa aaccagcagc caacgcgaac ttcttcccat
    16921 accataaagg tacgttacaa ggctgtaaga tgttttagaa agcagcggaa atgacgcagg
    16981 agtgaactta gaaaggataa ccgatggcga agttccagtt gatggtctta tcaggaaatg
    17041 tgatgcccag ttcattctgg taatacgctc ctttggggcc gtcaactgca ggatctacaa
    17101 gcgggtaagc agcgtccagg cggaaaataa aatacgagaa atccaggcga agacctgcac
    17161 ccggacctac tgccagttct ttgtaaaaat ctgacagctg gaatttagcg ccgggtctgc
    17221 tggcatcttc ctgcagagac catacgttgc ctacatcaca gaacaaggct cctttcagaa
    17281 taccggctat atcaaaacga tattctgcgt tcatttctat cttgacatcg ccggtacgat
    17341 cttccggctt cagggttgct acaccttcat cattgatctc atagctaaga tagcttccgg
    17401 ggcctacctt acgaatggga aaagcccgca ggctgttggc gccgcccaca tagaactgct
    17461 ttacaaaggg taccacctgt gaattccagt agggcacggc tatgccgaat gctgccctgc
    17521 ccaccagggt gttaccggtg ttgatgtcaa aataatcgcg gaaatcaaga tccagtctga
    17581 cgtagttgct gaaaggaata tcaaagatgc ggaaggggtc ggtcagctgg ctttctgaac
    17641 ccagtaaagg agcaccgttt ttgatggctg taccaatgaa gaataaagat cctgccagct
    17701 caccaccggc acggaagtac aagtcattgc ggctgtctgc aacggcctgg gtgttcagaa
    17761 tataggtagc attgggtgcc aggatgagct gctcctgaaa ggcctgacgc agcaggaaat
    17821 tgttctccag tatgctgtca aactgtgcgg tacttcttgg cacacgcaca tagttcacgt
    17881 ctattggata caggttgagt tgttgctttc tggagccctc aaagcgttca cttccatatt
    17941 tgaatgccgt gttgaagagt gtatagaact gcaccctgtc caggtattcc agcttgaggg
    18001 ttgtgatggt tcgcggatag tagttctttg gtgtgtacag agagaacggc cagaagaaac
    18061 gtgggaacaa caggttcacc tgggtggtga attcagaggt gttgagcgcc acattacggt
    18121 tggtgatctg cgactcgata ccaccatcca cagaaaaacg gagggcgtcc agtcgcctga
    18181 aagtgttcag cctcgtatag gaaaaacttc catagttggc cagcaggtat tctgatgtgg
    18241 tggatgtatt gaattcagtg gtgatgtcgt gtttgggcag cggctccagt ttgatggtga
    18301 ggtccatgga tcgtatctcg ccactggtgt caatcggggt ggtgctgatg gtggcatact
    18361 tgaacattcc caggttggag aaggcaccga tggtctggct caccttcgat tgtgtataat
    18421 aatcgccctg gttaataaag acattatcca ccagagattt gggtgtatag cgttcatgat
    18481 cctgcaggat gtagaatgct gaacggaatt ctccctttct ggtcttttca tcaaaacgga
    18541 tggtatcgtt cacggggccg ctcatatcag ataaaatgta gatgtctctg atcttgtagc
    18601 ggtggtgaat gctgtcattt tcaggtgggc tcaccttcac aaaaacgtca atattggcat
    18661 cagccacagt ggtatccacc tgaaaataca catattcttt attaaaatac agatatccgt
    18721 tatcagacag gtcatcggca atacgtttgc gttctgcttt cagaaagtcc acatcaaaac
    18781 gcctgccctg tttgagtggc gaagaggctg aatccagcaa agccagtctt ttcagttccg
    18841 ggtcatccag ttcatagacc acgcttctga tgcggtagag tttattcggt gtaacctcat
    18901 agtgtacggt agccctgtgt cttctttcat caacctctac actgaactga ctctgcggat
    18961 aaaagtaccc tctgttgtag aggtagttct ccatacggat acccgtatca tagatcagac
    19021 cggtatctat gagagaaggg gcctctccta ctttgacggt gagccattct ctgagttcac
    19081 tgtccggttg cagcggcggc gacaatgcca gccagggtgc aagtgaaagg ctgccctttc
    19141 ttgcagcagc ataatccaga tactgatcaa agcctctgtt gcccaggtta tacagccaca
    19201 ttttgagagg ataaatgccc agcacctttt tattaggctg cagtttactc agtctggcaa
    19261 ggtcattgtc cagccctttg agctcatcgg gtatgcgggt actgtcagaa aaagtgacct
    19321 ttgattcctt gtacagcacc tctccttcct gcagatacct ggcgttgctg caggcagaca
    19381 ggaacaggag caggaaaatg cccggcaagg aataacttcg cggtatatgc tgagtgctgc
    19441 tcaaataaaa cacctgactt cgctgcaact taagaaatac cgtcaaaagt atgctgaatt
    19501 tgtcattgaa ggcgggaaaa tcctggccga agctctgcag gagggcgtgg aagtaaaagc
    19561 tgtcatagct acccctgcac aactggataa atgccgcctg ctggcgggca acattcctgt
    19621 gctggaagca gatgagaaac agatgaaaaa tatttcttcc atgtctgagc ctccgggcat
    19681 cctggcctgg tgcagcatgc ggaaggcttc agagatgcag caacccgatg gctggcagct
    19741 ggcactggat ggcatccgcg accccggaaa cctgggaacc attattcgca cggccgactg
    19801 gtttggggtg cggaatatcc tgttgtctga agattgtgct gatatctata atcccaagac
    19861 cctgcagtca agcatgggct cgatgtttcg ctgcagcatt atacagggca cactgacaga
    19921 tctgctgcgg aaagcaaata agcctgtatt tgcagctgcc gccgatggtg attcaatgga
    19981 tcagttgcga actgcatctg gagtgttggt aattggcagt gaatcacatg gaatcagtga
    20041 agaggtcaag cgtgtttgcg atcattcggt tgccattccc ggcgatggca gaacagagtc
    20101 tctgaatgca gctgtggctg cggggatatt gttgtatggg atggtgaatt agtgtcaggt
    20161 agtgagaatt gagtagtggg tattgagaga aggcggaata cggaatacgg aatacggaat
    20221 acggaatacg gaatgcggag ggcggagggc ggaatgtgga atgaggagga cggagtgtgg
    20281 agtgtggagt gtggagggcg gagtaaggag tgtggggatg atgcgcttgt ataagagaac
    20341 aactcaaagg aatactgatt ctccggcctc cgagccggag tctccttcac tgatagaaga
    20401 gtgagactcc gggtcaagcc cggagagaca ttcatttgag ggtaaatcag ttattataaa
    20461 gattcggatc ttcccctttc tgtttcggct tgttgatcgg tgccttgtcg agggtctgat
    20521 caaagaccac acgctgcagt atccagtgct ttttcttttt atcaaagtag aacccctggt
    20581 aagtaccatc gggaatatag gtcatataaa taccttctga cagcgggttt tcgggctgca
    20641 gataatcaaa gatgatcatg ttcagcctgt catcccagac cattgcgggt gaagcatcct
    20701 ccttatattc gatgatgaca cgatctctga tgtcgccttc ttctatctcg aataccggct
    20761 taccaaattt tggcttgcct ccttcatcga accagagcac atccacgatc tttttattgc
    20821 tccacatgtc attcgcatcc cagccaaaaa gcagatagta tctttctttt ttaaatttaa
    20881 actcctgcac atcataatac agctgcccga accagttatc ctgatccagg ataacgctgt
    20941 ctatgcccgg aatgaacata ctcatatcaa tgagcggaaa aagcaccagt gaatcgctgt
    21001 gcatctggat agcaccgaaa tagcggtgag acagatcttt caattccatc tgccaggtaa
    21061 tgatgcgaaa ttctctgcta tccggata
//
LOCUS       DAOMAX010000013        21028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_260_length_21028_cov_4.751013, whole genome shotgun sequence.
ACCESSION   DAOMAX010000013 DAOMAX010000000
VERSION     DAOMAX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_21028_cov_4.751013"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PK742_01705"
     CDS             <1..587
                     /locus_tag="PK742_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQU75332.1"
                     /translation="KIQCRADLLLNENNIADLAAAGCDEIWIGAESGSQMILDAMDKG
                     TTIEQIRESTMLMKKHGIKPCFFLQFGYPGETRKEIRETIDLVTGLLPFDIGISVSYP
                     LPGTVFYDRVQAGLKQKTNWTDSDELAMMFAGTYSPEYYKKLHRYVHRRFRKAQGAWY
                     LKQVFKGEPTDLRRIMLWPYYAVRVMVDDQLVSD"
     gene            complement(584..3394)
                     /locus_tag="PK742_01710"
     CDS             complement(584..3394)
                     /locus_tag="PK742_01710"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQU75333.1"
                     /translation="MKRFIPVILLLGVWLPGKSQLDCTYGYTAMDLAETLVGSGIEIV
                     DATIDCYSQGFGLFECIDCNLGIDSGFILTTGKVINAEGPNNSGSLGYNSGLTGDPDL
                     NALPGVGTTYDRCVFELDIIPECDTISFDYVFGSEEYNEYVGSINDAFAFWISGPGIV
                     GMVNIALIPGTALPVTIDNVNLGSYPAYYHNNTGVPAGDPYYIQYDGFTVPLTAVSAV
                     TPGETYHLKLAIADEFDHSFDSGVFLKANSLGTSVTSLFSFPETGIGFFPEYCSSGLD
                     PGPELVPGAEIGIFTASPAGLVFVSDTTGVVDLSASTPGTYTITNTVITESCGLDTLS
                     SSVTIIINPAPVGNFSYPGTPFCNDAGSAPISLDPGSETGTFSATPAGLSINAATGTV
                     NVAASSPGTYTVTNTIPAGGGCDEDYFTTTIEIFPVYDLVTLAEICSGETYTLPSGLT
                     VSAAGEYVSSLTTDAGCDSVITTFLTVHPVFDVDVDAEICDGDTYVLPDGVSVTAAGT
                     YSSTLSSIYGCDSVVNTTLSVLPIPETSEVVYICDGDLYVLPDGVITGSAGDYTSVLT
                     AWTGCDSLVHTTLNLYPVYAVDVTAGICTGTTYTLPDGTVVSGGFYSTTLTTVDGCDS
                     IIYTTVEELDVIYTTIDEEICDGEEYILPDGSVTTVSGSYVNEYTTIFGCDSVITTNL
                     TVNPNPSLVFDLPEVVCYEQQIQPLIATPAGGSFTGSFVSGSTFDVLTAGVGGPYAIT
                     YTYTDANGCTSDTTMFIDVDANEASAFGSAEMIIGFGTPIYGITGGDYNWSPAESVAC
                     STCDSTIANPIQSGPIILTSYNNNGCVATDDIYITVLPDPEDYSFVPNTFTPNGDHIN
                     DHFTAYGPNLILVQNLRIFDRWGELIFLIEDFPANDPGIGWNGTTTNGNMVNAGVYSY
                     IMELKFIEGDKKTVAGNITLVR"
     gene            3495..3881
                     /locus_tag="PK742_01715"
     CDS             3495..3881
                     /locus_tag="PK742_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQU75334.1"
                     /translation="MNKKQVVLTAAAPAPIGPYSQAIAANGMLYVSGQIAIIPETGQL
                     IQTDNVAEETRQVMQNLGAVLEAGGSNFAQVVKCGIFLADMNDFAAVNEVYGAYFTSD
                     PPARETVEVSRLPKGVRVEISAIALL"
     gene            complement(3859..4785)
                     /locus_tag="PK742_01720"
     CDS             complement(3859..4785)
                     /locus_tag="PK742_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQU75335.1"
                     /translation="MQYQARHWFILIFLSVVWGTSFILMKNGLETFSWDQVAALRISV
                     SFIGTLPFLFLFGRHIKGKQLKYSALTGLTGSGIPAFCFTFAQTHIESGIAGVLNSLT
                     PAFTFALGVLFFAMPFHRWKLAGLLTALTGALILVSSDPDGGHSQLWYALPVFLATLS
                     YATSANLVKTYLQDAHPIGLGAVGFASIGIPSLLYLLSTRFWELAAETTYPEMSAISH
                     FSISLWSIVALSLFGTVMASIIYFKLIQMTDSLFGSLVAYIIPVVALLLGVWDGERIS
                     MTHVLGMLFILAGIYMINARQPFALVRVRRSH"
     gene            complement(4851..5843)
                     /gene="obgE"
                     /locus_tag="PK742_01725"
     CDS             complement(4851..5843)
                     /gene="obgE"
                     /locus_tag="PK742_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQU75336.1"
                     /translation="MDNQNFVDYVKILFRSGKGGAGSMHFHRSKLTAKGGPDGGDGGR
                     GGHIILKGNSHMWTLLHMKYQKHVFAGDGQSGAAALKTGKQGEDRIIEVPIGTIARDP
                     ETGEVFGEVMADGEELVLLQGGRGGLGNNHFKTAVNQAPRYSQPGEEGTEAWIVLELK
                     VLADVGLVGFPNAGKSTLLSVLSAAKPEIADYPFTTLTPKLGIVSYRDNRSFVMADIP
                     GIIEGASEGRGLGTRFLRHIERNSALLFMVPAEAKDIHQEYRILLHELKQHNPELMTK
                     SRMLAITKSDLIDDELEMMLSKDLPDVPVVFISSHTGKNMDKLKDMLWNILVND"
     gene            complement(5855..6433)
                     /locus_tag="PK742_01730"
     CDS             complement(5855..6433)
                     /locus_tag="PK742_01730"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQU75337.1"
                     /translation="MKNIILFGPPGSGKGTQAAMLKDHFNLLHISTGDLLREEIRKGS
                     VLGTEAKQYMDRGELVPDAVVIGMIGGSFDTAREEGKEGIIFDGFPRTVAQAEALDRL
                     MEDKDTSIAGVLSLVVDEEELTKRILNRGLTSGRSDDSDEATIRNRVQEYRTKTEPVA
                     SYYAANDKVREIEGVGSIDDIFQALCKAFEDL"
     gene            6511..7458
                     /locus_tag="PK742_01735"
     CDS             6511..7458
                     /locus_tag="PK742_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQU75338.1"
                     /translation="MQTIIGAGGTIGKPLARELKQYTQAIRLVNRHPQHVNGDDELFS
                     ADVTDPDMLDKAIAGSEVVYCTIGFKYELKTWQQTWPSFMKHLTASCLKHGSKLVFFD
                     NVYMYAASSIPDMNETSAVDPPSKKGQVRAEVVSILEEAGRKGLTWIIARSADFYGPE
                     NRQSVLVETVLKNQLKGKKAQWLGKIDKVHQYTYTPDAAKAVAMLGNAPDTWNQVWHL
                     PTDQVSMTAREWVSLFAGYTGSKDGIQVAPPWMVSVMGWFMPIMKEIREMMFQNMQHY
                     RFHDEKFMQRFPDFRVTPAAEGVEETVKWFRNHPLEVGG"
     gene            complement(7459..8547)
                     /locus_tag="PK742_01740"
     CDS             complement(7459..8547)
                     /locus_tag="PK742_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQU75339.1"
                     /translation="MLLRNATLLLLAWGSFACRPAANTRNVIPSTADTLSVLASGLHV
                     PWGMDLLPDGNLLFNQRDGAICLLNRTSGMVTVLMERQVNDQAEGGLLGLAADPDFLS
                     NSYIYIYETVAAGNQVVRLQLQGHTLRQDSIILSGIPHAKFHDGGILRFGPDSFLYVG
                     TGDARQPDLAQDRNSLAGKILRIDRNGTPADGNPFGNAVFSYGHRNVQGLAWSEQGQL
                     YATEHGPSGELNGWCCHDELNKIIAGGNYGWPMVTGDDERKGCIAPLAHSGEDTWAPG
                     GLVWVNENGPETLRGKLVFACLRGRKLLVFDTDGHEQQVLFDGTFKRLRNIIQIPDGS
                     LVFCSSNKDGREALPLQEDDHIYLLPAR"
     gene            8606..8989
                     /locus_tag="PK742_01745"
     CDS             8606..8989
                     /locus_tag="PK742_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75340.1"
                     /translation="MYKYILGAFFTFMAVMFVRCGEVTEIDELVKQTERVQIVYNTNL
                     GAYQDITDKKEIRAFSDYILDEPTPLYKCGYDGYMIFFTEHGSVRMDFNLQDDCIHVL
                     YPYGETLQTRLISKEGVAYLRSQAP"
     gene            8993..9742
                     /locus_tag="PK742_01750"
     CDS             8993..9742
                     /locus_tag="PK742_01750"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HQU75341.1"
                     /translation="MAGKAQFPRVPTLLLMNLFPLAGVLFFNWSFFAIIYIYWWETVI
                     LSFFNVLKMAKAAKRAEARPNVTINGAPETESIRNNKPLIMVTYMGTRAFILFIYLVF
                     ILVFIGMYQGNRPGQPSMGEIILFMDPFMKAGIVAVLIYYLTDYLVWRFTGDWQETTA
                     ASLAMPFDGRSIVMHVVIVLGAVSTAFLGETLFGSEQKAAPILFASFFVIMKTAVDLW
                     AESGKKDRETVVLETLGKKRGNDDARPTTQG"
     gene            complement(9739..10254)
                     /locus_tag="PK742_01755"
     CDS             complement(9739..10254)
                     /locus_tag="PK742_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQU75342.1"
                     /translation="MQPHRLRLLFSKDQIEAAVGMLADQLNQRFAEKDPLFLVVMNGA
                     YMFATDLLKQFIHPCTLAFIDVKSYSGTTRFVLDADSSRIPDLTNRHVVILEDIVDSG
                     TTMAWLKKETAGCGAASVTSAALLNKLVPHEADADLYGLDVEDLFVVGYGLDLDGRYR
                     NLPQIFELLQD"
     gene            complement(10270..11634)
                     /locus_tag="PK742_01760"
     CDS             complement(10270..11634)
                     /locus_tag="PK742_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HQU75343.1"
                     /translation="MKNTLLAFTLLTGQFSLFPGDPNEAVLAYIERYRYIAVEEMINH
                     GIPASITLAQGILESGAGRSQLASESNNHFGIKCHDGWDGERVYHDDDAKGECFRKYS
                     NPEESYRDHSMFLLTRQRYAGLFELDPNDYKGWAKGLKAAGYATNPQYADRLIALIED
                     YKLYEYDELSPDQLLAMEEHKKVDKSKEEKKSDADKKDATNQTTAVFYYNRIPTVVVN
                     PGETLELLSERHNIRMKRLLEYNNMTGTEKLEPGTYVYLQPKRKKGLTKYHKVESGET
                     MWTISRDEGVRLDRLYKYNLLTPGQEPAAGQLIYLRKERETAPMLLDKVKELNDKEKR
                     EEMRRQKEADDKKALEQQEEEQRKKNEILMEFEESGAPRKSESPGARLDTDPVIPDVM
                     SVKPFLVAEELPVPLYHYVEPQETLYGLARVFNTTVDNLKSWNQLGDNTIKAGQQLII
                     GYRE"
     gene            complement(11718..12359)
                     /locus_tag="PK742_01765"
     CDS             complement(11718..12359)
                     /locus_tag="PK742_01765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU75344.1"
                     /translation="MTYILPPSLDAYIEQHSSKPDEHLQQLDRETHLKVLQPRMLSGA
                     VQGKFLEFISTMIRPKRILEIGTYTGYSGICMAKGLVEGGTITTIDINEELSSMVRRY
                     VDAAGLTEKFDIRTGDATRIIPELSGPFDLVFIDADKRNYSRYYELVIDKVPAGGWIL
                     ADNVLWSGKVVEEATDKDTTAIRAFNELVQADERVENVLLSVRDGIMIVRKNG"
     gene            12424..13980
                     /locus_tag="PK742_01770"
     CDS             12424..13980
                     /locus_tag="PK742_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQU75345.1"
                     /translation="MRFVLLLTISLLSFTVSAQLNITWVSSLPYSQLSSLWGYTAPDN
                     TEYALVGAYNGLSIVSLADPAAPEELFFVEGAESAWREVKTFGHYAYVTNETGGGLLI
                     VDLQDLPGAIDTVSYAGTADHPFTTAHTLFVDENGILYLFGYNVVFGLTGAYMLDLNT
                     DPMNPDFVAVYEDHYIHDAFVRNDTMWAAEIYDGRFEVLDISDKTSFLSLGYAETYGV
                     ATHNCEPDASGNILFTTDEIFNGYITSFDISDLTDIQELDRYKHGDTALAIPHNVRVL
                     GDFLVASHYSEGVVILDASRPGLLVETGHYDTNPAGPDAFFEGAWEVYPYLPSGLLIV
                     SDILEGLQVLQPEYVYAAFLEGVVTDEVTGASIPLATIELTGTTATAQADLLGQFELG
                     YHSAGIYDVSVSAPGCSTRVFADVELENGVTAILDAALDCGTVALPAVPDCSGWLAGT
                     DHSTGSVWIQNYCTESNTTCTMTDLNGRMIWSGSVSGTDRYQIPVTTPPGIYFLADQL
                     GQTRSVTVMH"
     gene            14047..15033
                     /locus_tag="PK742_01775"
     CDS             14047..15033
                     /locus_tag="PK742_01775"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQU75346.1"
                     /translation="MRWLLLLCLYAGMVQAQLLPSYGDSRTATTGWQFLKVVPDARSA
                     GMGEAFLAVADDMGSVYWNAAGLSKLDTSRWHTMLAHTAYPASINQYYGALAWRLNSN
                     TLFAGSLQYFDAGSMPVTTEFQPAGNGMTFHPVNMALGASWSQVLTDAFSFGVTLKYV
                     REDIADVHNQNMLFDLGFRYDIGIARTRFAVALTNFGFNTTPDGSIPVLSITDTTVIV
                     QAEDVGVPTIFRLGFAWDALASETHQLTAAAQLNHPTDNNETYSIGLEYGWKKLLFVR
                     SGYQFATDLAAWPSFGFGVRVPRYFGTVQLDYGFQYMNRLGTVNRIGLQLSL"
     gene            15030..16244
                     /locus_tag="PK742_01780"
     CDS             15030..16244
                     /locus_tag="PK742_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75347.1"
                     /translation="MKKLAYILICFPLLSSCDFLFGTTKDDVAAEIFEEGAIDPELIP
                     SEVGYVPVLPFWTGFDHPVDIYCGYDEMIYVIDDNGLNVLDQAGNLHDIIYIQGASDV
                     CQDRRLHTYVTGRVDVDVDDDGMPENLAAVYHLTGTATGNVQFIDTLIHPFCDESRNI
                     TSFRGTDDIAVEFTGVTTLADNTLHVSRTGPRNDLTGIARVDNTILFFDEDGINTGYS
                     NGLSPVSSNLRSSWNISSIAGFAAPPQSLAGISTSADFLITLEAEDAAYKVLWIQQNV
                     DPEAGVTYGENAALAAFDLTKADRFLYEPGRFEAPADVYVAPDFTGYIFVVDSELDSL
                     YQFTRRGYEGVNPPPGSTTTKQIIASFGGAGDGPFNFNEPSGVAYLRRVVYVADKGNG
                     RICRYTLSTDLE"
     gene            16267..17394
                     /locus_tag="PK742_01785"
     CDS             16267..17394
                     /locus_tag="PK742_01785"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQU75348.1"
                     /translation="MSKQIVVLGAGMVGRAMAIDLAKDFQVTSADISRENLDLLLPFG
                     IHTIQADLSDAAAIQELIAAADLVIGAVPGFMAFRTLQTVIEAGKNICDISFFDEDPF
                     LLDQLAKDKGVTAIMDVGVAPGMDNILLGYHHRRMQVNRFVCLVGGLPKHRQWPYEYK
                     APFSPIDVLEEYTRPARYVQDGKEVVMPALSEPELMEFAEVGTLEAFNSDGLRSLIRT
                     MPDIPNMIERTLRYPGHIELMRVLRETGFFSKEAILVKGQAIRPIDLTSQLLFPMWKL
                     EQEEEEFTVMRVIIEGTENGSHRTYTYDLLDRYDAATKTSSMARTTGYTCTGAARLVL
                     NGMYDKKGVFPPEFLGEEEAHLSFMLEHLAQRNIHYRQQIS"
     gene            17441..20554
                     /locus_tag="PK742_01790"
     CDS             17441..20554
                     /locus_tag="PK742_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU75349.1"
                     /translation="MKRLLSVLCFLLFYCAALRAQLPDLYFEQITTRNGLSNNSITCL
                     FEDSRGFIWIGTRFGLNRYDGQTYEHFLKSNQNRNSISGNYIVRVLEDQDGIIWVATK
                     DGGLNRFDEQAKEGFRWTALMESTDNKTGLPSNRLTDIVMLNDSVLLLTAEVNPLILL
                     HTRNMEFRYIAPAKNVTDAVLSVRWDDAGSEIKDPFWIHRFKTFRGQIFLSPLKPGPV
                     AAIDATGKLVLRFRDLPCTTIAEFLPEGDSLWVSGWKGGLFLQRNSGTGDDPVITEVF
                     DAEDQINCIESLDDHTLLLGMNSTGVALLDKRTMKSRFFMSEVNNAYSIPGNRVLAMM
                     IDRNGIVWIGTTKGLARYNPAQWKIHAELLPYDEARDISYYSVFTESSGTLRICSSAG
                     IFKKEPADAFFRRVELDFKGQAIQVTGIVQLSDGRFMLSSEQGLFWYDPVKEQLSDWI
                     PSLVYLRDTVIINRSHYSRIAFQVRSPVVYKDQDKEFVLAAVIGYGLLVYDVSDNVNY
                     FLVRDEKNPLSFGDGLLLNVAIDKDRQVWLGSTQGLYRWRSEEGLVNNFDRFLHDPDD
                     PASIPSNYITSIISDPEKGVWITTPDGLGRLVDGSCQTWLPGNDLHRVMLGGLYDQGK
                     VWAAVLEGFEIFDVKRKTFRYQGIPHESWYPSAPLGIIKLPDGQFAYASGKHFLTFHQ
                     DDLIADRSFPDVFIKTLLVQDSLIYLKEYPDRIVLKHNQNFVQFNLAGIQVRYPGSLK
                     IRYRLTGLEENWTESSVTSFIRFTNLTPGSYALEVQVTNPVGEWSETNTLYLLDISKP
                     WYGTWWFYGLCLVMSLSVFRLIIFLRERSRYQIEMIRNRIANDLHDDVGSALSSIHLY
                     SEVAHNHSQKNQQELRSLLQKISHASLEMQENMGHIIWSLQPRNAPAGQMGARLRQLA
                     GEMLGAANIRFTYEWDDNLGSLHLSPVQRKECYLICKEALHNIIKYAACTKVTIRLYR
                     KSESMCVDISDNGKGFDKESVQYGNGLYSMQQRAESLKGSLKITSQPGDGVLVSLCFP
                     AGTRKIW"
     gene            20589..>21028
                     /locus_tag="PK742_01795"
     CDS             20589..>21028
                     /locus_tag="PK742_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU75350.1"
                     /translation="MKEIRVALFDDNDIRRDGVELLLKTTEGYVCCGSYEHAGNLVAE
                     LHDAQPDVVIMDIDMPGIDGIEAVRILKGAFPNLPVMMQTVFDHDEKVFESILAGATG
                     YLLKKTPPVKLLEAISEMHDGGAPMTPEIAAKVLAFFAKQNTPEP"
BASE COUNT         5255 a         4987 c         5321 g         5465 t
ORIGIN      
        1 tcaagataca atgccgtgca gatctgctgc tcaatgaaaa caacattgcc gatctggctg
       61 ctgcaggttg tgatgagatc tggataggtg cagagagcgg ctcacagatg atactcgatg
      121 ccatggataa aggcacaacc atagagcaga tcagagaaag caccatgttg atgaaaaagc
      181 atggtattaa accttgtttc tttttgcagt ttggttatcc gggcgaaacc aggaaggaga
      241 tcagagaaac cattgacctg gtaacagggc ttctgccttt tgatattgga atttctgtaa
      301 gctatccctt gccaggtacg gtcttttatg atcgtgttca ggcaggcctg aaacaaaaga
      361 ccaactggac agacagtgat gagctggcta tgatgtttgc cggcacatat tctcctgaat
      421 actataagaa actgcatcgg tatgtacacc gccggttcag aaaagcacag ggagcctggt
      481 atctgaagca ggtgttcaag ggtgagccga ccgatctgcg ccggatcatg ttatggccat
      541 actatgctgt ccgggtgatg gttgatgatc agctggtaag tgattagcgc accagggtga
      601 tattacctgc tacagttttc ttgtcgcctt caatgaactt cagttccatg atgtaactat
      661 acactcctgc attgaccata ttgccattgg tggtagtgcc gttccagcca atacccggat
      721 cgttggccgg aaaatcttca ataaggaata tcagttcacc ccatcgatca aagatgcgca
      781 gattctgcac cagtatcaga ttaggaccat aggctgtgaa atggtcattg atgtggtcgc
      841 cgttcggggt gaaggtattt ggcacgaaag aataatcttc cggatcgggc agtacggtaa
      901 tataaatatc atcggtagct acgcatccgt tgttgttgta tgaggtaaga atgataggtc
      961 ctgactggat cgggttggca atggtactgt cgcaggtaga acacgccact gattctgccg
     1021 ggctccagtt ataatcgccg ccggtaatgc catagatagg cgttccaaaa cctatgatca
     1081 tttctgctga cccgaaggca gatgcttcat tagcatccac atctatgaac atggtggtgt
     1141 cactggtgca gccatttgca tctgtgtagg tataggtgat ggcataaggc ccgccaacac
     1201 ctgcggtcag cacatcaaat gtgctgccgc ttacaaatga accggtaaag cttcctcctg
     1261 ccggtgtggc aatgagtggt tgtatttgtt gttcatagca gaccacttca ggaagatcaa
     1321 agacaagtga ggggtttggg ttcactgtaa ggttggtagt gatcacagag tcgcagccaa
     1381 aaatggtggt gtattcattc acataagagc ctgaaacggt agtaacggaa ccatcaggca
     1441 ggatatattc ttcgccatca cagatctctt catcgatcgt ggtatagatc acgtccagtt
     1501 cttccacagt ggtataaatg atagaatcac aaccatctac agtggtcagg gtcgtactgt
     1561 aaaatcctcc ggaaaccaca gtaccatcag gcagggtata ggtggtaccg gtgcatattc
     1621 ctgcggtcac atcaacggca tagaccggat acagattcag tgtggtatga accagagagt
     1681 cgcagcctgt ccaggctgta agaacagaag tataatcgcc ggctgaccct gtgatcacgc
     1741 catcaggaag cacgtacagg tctccgtcgc agatgtacac cacttcgctt gtttcaggaa
     1801 taggcagcac agacagggtc gtatttacaa cagaatcgca gccgtatatg ctgctcagcg
     1861 tgctgctgta ggtacctgca gcggtaactg atacgccgtc aggtaagaca taggtatcgc
     1921 catcacagat ctctgcatct acatctacat caaataccgg atgcacggtc aaaaaggtgg
     1981 taatgactga atcacaaccc gcgtctgtgg tcagggaaga tacatattcg ccggcggcag
     2041 aaacggtcag accgctgggc agggtatagg tctcgccgct gcatatctca gccagagtga
     2101 ccagatcata caccggaaat atttctatag tggtagtaaa atagtcttca tcacatccgc
     2161 caccagcagg aatggtattg gttacggtat aggtgccggg acttgaagca gccacattga
     2221 cagtacccgt tgcagcattg atgctcaggc ctgccggggt agcactgaat gttccggttt
     2281 ctgatccggg atcaagtgaa atgggtgcac tgcctgcatc gttgcagaaa ggtgtacccg
     2341 ggtagctgaa attgccaacg ggtgccggat tgataataat ggttacactg gatgaaagag
     2401 tatccagacc acagctttca gtaatgacgg tgttcgtaat tgtataggta cccggagtag
     2461 aagcactgag gtcaactaca cctgtagtat cagaaacaaa cacaaggccg gcaggacttg
     2521 cggtaaaaat gccaatttca gctccgggaa caagttccgg ccccggatca aggccggagg
     2581 agcagtattc agggaaaaat cctatacctg tttccggaaa agaaaacaaa gaggtgacgc
     2641 tggttcccag gctattggct ttcagaaaaa cacccgaatc gaaagagtgg tcaaactcgt
     2701 cggctatggc cagtttcagg tgataggtct caccgggagt gacggctgat acagcagtga
     2761 gcggaacagt aaagccatca tactggatgt agtaagggtc gccggcaggt acaccggtgt
     2821 tgttgtggta atatgccggg taactgccca gattcacatt atcaatggta accggcagag
     2881 ctgttccggg aatgagagcg atgttgacca tgccaacgat gccgggcccg ctgatccaga
     2941 acgcaaaggc atcattgata gaacctacat attcattgta ctcctcactt ccaaagacat
     3001 aatcaaagga tatggtatca cattccggta tgatatccaa ttcaaataca cagcgatcgt
     3061 aagtagttcc tacacccgga agtgcattca ggtcaggatc gccggtcaga cctgagttat
     3121 accccaggct gccggagttg ttgggacctt ctgcattgat cactttgccg gtggtcagaa
     3181 tgaacccgga atcaatgccc aggttacaat caatgcattc aaaaagtcca aaaccctgcg
     3241 aatagcaatc aatggtcgca tccacgatct caatacccga acccaccagg gtctcagcca
     3301 gatccattgc agtgtagccg taagtacagt ccagctgtga cttacccgga agccagaccc
     3361 ccagtaacaa tatgacaggt ataaatcgtt tcattggcag gtttcatgaa cctataaaaa
     3421 taataaatca gcagattttt taacaaaacc gccctggctg acagcaaact gtgttttttt
     3481 accattttta ctgtatgaac aaaaaacagg tagtgctcac cgctgctgcg cccgctccta
     3541 tcgggcctta ctctcaggcc atcgcagcca acggcatgtt gtacgtgtca gggcagatcg
     3601 ccatcatacc ggaaaccggc cagttgatcc aaaccgataa cgtggctgaa gaaaccagac
     3661 aggtcatgca gaatcttggc gccgtactgg aagcaggggg gagcaacttt gctcaggtgg
     3721 tgaagtgcgg aatttttctg gctgacatga acgactttgc ggcggtcaac gaagtatatg
     3781 gtgcgtattt cacgagcgat cctcctgcaa gggagactgt agaagtgtcc aggcttccga
     3841 aaggtgtccg ggtagagatc agtgcgatcg ccttactctg accagtgcga atggttgtct
     3901 ggcattgatc atatagatgc cggccagaat gaaaagcatt cccagtacat gagtcatgga
     3961 aatgcgttca ccatcccata cacccagcag caatgccacc acaggaatga tataagctac
     4021 cagtgagccg aataacgaat cggtcatctg gatgagtttg aagtaaatga tgctggccat
     4081 taccgtgccg aacaatgaca gggcaacaat gctccaaagg cttatggaga aatgagatat
     4141 ggcagacatt tccggatagg ttgtttcggc agccagttcc cagaagcggg tgctgagcaa
     4201 atagaggagt gatggtattc ctatgcttgc aaaacccacg gcacccagcc cgatcgggtg
     4261 tgcatcctgc aggtaggtct tgaccaggtt ggcacttgtg gcataagata aggtagccag
     4321 aaaaactggc agtgcatacc acaactgact atgacctccg tccggatctg atgacaccag
     4381 tatcaatgca cctgtcagtg cggtcagcaa tccggccagt ttccagcgat ggaacggcat
     4441 ggcaaagaac aataccccga gtgcaaaagt aaaagcagga gtcaggctgt tcagtacccc
     4501 tgcaatgcca ctctcaatat gtgtttgtgc aaaggtgaaa cagaaagcag gaataccgct
     4561 accggtcagg ccggtcaggg cagaatactt cagttgtttt cctttgatgt gccgtccaaa
     4621 aagaaataaa aatggcaggg tgccgatgaa ggaaaccgag atgcgcagcg cagctacctg
     4681 gtcccagctg aaggtttcca gcccgttctt cataaggatg aaggaggtac cccacaccac
     4741 cgacaggaat atcagaatga accagtgtct ggcttggtat tgcatggatc aggttatact
     4801 gctgtgtgtt gcgccggtca aaaaatactg caggtcatga acaggatgca tcagtcgttc
     4861 accagaatgt tccacaacat atctttcagc ttatccatgt tcttgcctgt atgactgctg
     4921 atgaacacca ccggaacatc aggcaggtct ttgctcagca tcatttccag ttcatcatca
     4981 atcaggtctg atttggtaat ggcaagcatg cgtgatttgg tcatcaactc cggattgtgc
     5041 tgcttcagtt cgtgcagcag aatgcgatat tcctggtgta tatcctttgc ttctgcaggt
     5101 accatgaaaa gcagggcgct gttgcgttca atgtgtctga gaaacctggt gccaagtcct
     5161 cttccttcac tggcaccttc tatgataccc ggtatgtctg ccatgacaaa gctcctgttg
     5221 tcacggtaag aaacgatgcc aagtttaggg gtcagcgtag tgaaagggta atctgcaatt
     5281 tccggtttcg ctgcgctgag taccgacagc aaagtggatt ttcccgcatt cggaaagcca
     5341 accagtccaa catctgccag tacctttagt tccagcacga tccaggcttc agtgccctct
     5401 tcgccgggtt gcgagtagcg tggcgcctga ttaacagctg tcttgaaatg gttgttgccc
     5461 aaccccccgc ggccgccctg aagcaatacg agttcttcgc catctgccat gacctcacca
     5521 aacacctcac ccgtttccgg atctctggca atggttccaa tgggcacttc aatgatgcgg
     5581 tcttctcctt gtttgcccgt cttgagtgct gctgctccag actgaccatc gccggcaaag
     5641 acatgcttct ggtatttcat gtggaggagg gtccacatat ggctgttgcc tttcaggatg
     5701 atgtggccgc cacgtccgcc atcaccgcca tcaggcccgc ccttggcagt cagtttgctt
     5761 cggtgaaaat gcatgcttcc tgctccgcct tttccactgc gaaacaagat cttgacataa
     5821 tcaacgaagt tctgattgtc cattgcccgg cagtttacag gtcctcaaag gccttacaca
     5881 acgcctggaa tatgtcatca atgctaccta ctccttcaat ttctctgacc ttgtcattgg
     5941 cagcataata agacgctacc ggttccgtct tggtgcgata ttcctgcacg cggttgcgga
     6001 tggtagcttc gtctgaatcg tcgctgcggc cactggtgag tccgcggttc agaatacgtt
     6061 tggtcagttc ttcttcatct actacgagcg acagcacgcc tgcaatggaa gtgtctttgt
     6121 cttccatcag tctgtctaat gcttctgcct gtgccactgt tctggggaaa ccgtcaaaaa
     6181 tgattccttc tttgccttct tcgcgggccg tatcaaagct tccgccaatc ataccgatga
     6241 ccacagcatc gggtacaagc tcgcctctat ccatgtattg tttggcttcc gtgcccagta
     6301 ctgagccttt ccggatctct tcacgaagaa gatcgccggt agagatgtgc agcaggttaa
     6361 aatgatcttt cagcattgcg gcttgggtac cttttccgct acccggagga ccaaagagaa
     6421 tgatgttctt catcgtcagc ttcttaggag tgcaaaggta cgcaatcaga agccttaaaa
     6481 tatttttgct tttcaatcgt ctttagggtt atgcaaacca tcataggtgc aggtggtacc
     6541 atcggaaaac cgcttgccag agagctgaag caatatactc aggccatcag actggtcaac
     6601 cggcatccgc agcatgtgaa cggcgatgat gaactttttt ctgccgatgt gaccgatcct
     6661 gatatgctgg ataaagcaat agccggcagt gaggtggtct attgtaccat aggtttcaag
     6721 tatgagctga agacctggca gcaaacctgg ccttctttca tgaaacatct cacagcatcc
     6781 tgtttgaaac acggcagcaa actggtcttc tttgataatg tgtacatgta tgcagcttct
     6841 tccattccgg atatgaacga aacctcagct gttgatcctc ccagcaaaaa aggtcaggta
     6901 agggcggaag tagtaagtat tctggaagaa gccggaagaa aggggctgac atggatcatt
     6961 gccagaagtg cagattttta cggccctgag aacaggcaga gtgtgctggt ggagaccgtg
     7021 ctgaaaaatc aattgaaggg taaaaaggcc caatggctgg gaaagattga caaggtgcat
     7081 cagtacacct atactcctga cgcagcgaag gcagtggcca tgctgggtaa tgcacccgac
     7141 acctggaatc aggtctggca tctgccaacc gaccaggttt cgatgaccgc cagagaatgg
     7201 gtctccttat ttgcaggata taccggaagc aaagacggca ttcaggtggc tccgccctgg
     7261 atggtcagtg tcatgggatg gttcatgccc atcatgaaag agatccggga gatgatgttt
     7321 cagaatatgc agcactaccg ctttcatgat gaaaagttca tgcaacgttt tcctgatttt
     7381 agagttacgc cggctgccga aggtgtagag gaaacggtaa agtggttcag aaatcatcct
     7441 ctggaagtgg gtggctgatc atctggctgg cagtagatag atatgatcat cttcctgtaa
     7501 tggcagagct tctcttccat ctttgttgga agagcaaaat accaggctcc cgtcaggtat
     7561 ctgaatgatg ttacgcaatc ttttgaaggt accgtcaaac agcacctgtt gctcgtgtcc
     7621 gtcagtatcg aacaccagta gtttccttcc ccgcaggcag gcgaacacca gttttccgcg
     7681 aagcgtttcc ggtccgtttt catttaccca caccaaacca ccgggtgccc aggtatcttc
     7741 tccgctatgt gccagtggcg caatacagcc tttccgttca tcatctccag ttaccatcgg
     7801 ccaaccgtaa ttaccgcctg caattatttt attgagttca tcgtgacaac accagccgtt
     7861 cagttcgccg gaaggaccgt gttctgtggc atacagttgt ccttgttcac tccatgccag
     7921 tccctgcaca ttgcgatgac catagctgaa cacggcattt ccaaaaggat taccatccgc
     7981 cggagtacca tttctgtcta tgcgcaggat ctttcctgcc aggctgttcc tgtcttgtgc
     8041 taggtccggt tgacgggcat cgccggtgcc aacatagaga aagctgtcag ggccgaagcg
     8101 caggatgccg ccatcgtgaa attttgcatg tggaatgccg ctcagaatga tgctgtcctg
     8161 ccggagtgta tggccttgca actgcaacct gaccacctgg ttaccggcag caacagtttc
     8221 ataaatataa atatatgaat tggataaaaa atcaggatca gcggccagtc cgagcagccc
     8281 gccttctgcc tggtcattta cctgtcgttc catgagaact gtaaccattc ctgatgtcct
     8341 gttcaggagg catatggcac cgtcacgctg gttgaagaga aggttgccat ctggtaacag
     8401 atccatgccc catggaacat gcagacctga tgccagtaca gataaggtgt ctgccgttga
     8461 tggaattacg ttccgggtgt tggcagcagg ccggcaggca aaactgcccc acgccagcag
     8521 cagaagtgtt gcattgcgca atagcatatt ccaaggtacg ttttttcagg caggggtatt
     8581 cctgtttgaa ccgtatcttt agtccatgta caaatacata ttaggagcat tttttacatt
     8641 catggcggtc atgtttgtgc gttgcggaga agtgaccgag atagatgaac tcgtcaaaca
     8701 aacggagcgg gtgcagatcg tttacaatac caaccttggt gcttaccagg acatcacaga
     8761 caaaaaagag atcagggcat tttcagatta catactggat gaacctactc ctttgtacaa
     8821 gtgcggatat gacggttaca tgatcttctt taccgagcat ggcagtgtac ggatggattt
     8881 caacttgcag gatgattgca tacatgtgtt gtatccgtac ggagagacat tgcaaaccag
     8941 gctgatatct aaagaaggtg tagcatacct gcgttcacaa gctccctgat ccatggccgg
     9001 caaagcacag tttcccagag ttcctactct cttgctgatg aacctgtttc cgctggctgg
     9061 agtcttgttc ttcaactgga gtttttttgc catcatatat atctactggt gggaaacggt
     9121 cattctgagt ttctttaatg tgcttaagat ggccaaggca gctaagcggg cagaggcaag
     9181 gccaaacgtg accatcaacg gggcaccgga aacagagtct atacgcaaca ataagcccct
     9241 gatcatggta acctacatgg gaacccgggc attcatcctt ttcatttacc tggtttttat
     9301 tctcgttttt attggtatgt accagggaaa ccgtccggga caacccagta tgggcgagat
     9361 cattttgttc atggatccat tcatgaaagc aggtattgtg gctgtgctta tttattatct
     9421 gacagattac ctggtatggc ggtttaccgg cgactggcag gaaaccactg cagcatcact
     9481 ggccatgcct tttgacgggc gttccattgt catgcatgtg gtcattgtgc tgggggcagt
     9541 tagcacggcc tttctgggtg aaaccctctt tggttctgag cagaaagcag caccaatttt
     9601 atttgccagt ttttttgtga tcatgaagac tgctgtggat ctctgggcag aatcaggtaa
     9661 gaaagacaga gaaaccgtag tattggagac cctcggaaag aaacgcggga acgatgatgc
     9721 ccgccctacc acacagggtt aatcctgtaa tagttcgaag atctgaggaa ggttgcgata
     9781 gcgaccgtcc agatccagtc cgtaccctac aacgaacagg tcttccacat ccagtccata
     9841 cagatctgca tctgcttcat gtggcacaag cttgttgagc agcgctgctg aagtaactga
     9901 tgcagcaccg cacccggcag tttctttttt aagccatgcc atggtagtac cgctgtcaac
     9961 aatatcttcc agtatgacca catgtctgtt ggtcagatcc ggtattctgc tgctgtctgc
    10021 atcaagcaca aaacgtgtag tgccactgta gcttttcacg tcaataaatg ccagggtgca
    10081 ggggtgtatg aattgtttga gcaggtctgt ggcgaacatg tatgcaccgt tcatgaccac
    10141 aaggaacagc ggatcctttt ctgcaaatct ttggttaagc tgatcggcca gcatgccaac
    10201 ggctgcttcg atctgatcct tgctgaacag cagccggagc ctgtgaggtt gcatgcccaa
    10261 agataggcct tactccctgt aacctatgat aagctgctgg cctgctttaa tggtgttatc
    10321 acccagctga ttccaggact tcaggttatc taccgttgta ttgaacacac gtgccagtcc
    10381 gtatagagtt tcctgtggtt ccacataatg atagagcgga accggcagtt cttcagcaac
    10441 aaggaatggt ttaacgctca tgacatcagg aatgaccgga tcggtatcca gtctggcgcc
    10501 aggcgattca ctttttctgg gtgcaccgga ctcttcaaat tccatcagga tctcattttt
    10561 ctttcgctgt tcttcttcct gctgttccag tgctttttta tcgtctgctt ctttctgacg
    10621 gcgcatttct tcccgctttt ccttatcgtt cagttctttc actttgtcca gtaacatagg
    10681 ggcggtctcc ctttccttgc gcagatagat gagctgaccg gcagcaggtt cctgacccgg
    10741 agtgagcagg ttgtatttat acaggcgatc cagtctgaca ccttcatcac ggctgatggt
    10801 ccacatggtc tctcctgatt ctaccttatg gtatttggtg aggccttttt tgcgtttggg
    10861 ttgcaggtaa acgtacgtac ccggttccag tttttctgtt ccggtcatgt tgttgtattc
    10921 caacagacgt ttcatgcgga tgttatgtct ttcagacagg agctcaagtg tttcaccggg
    10981 gttgaccact accgttggaa tgcggttgta gtagaaaaca gcggtagttt gattggtggc
    11041 atctttttta tctgcatcac tcttcttctc ttccttgctc ttgtcaactt tcttatgctc
    11101 ttccatggcc agcagctggt ctgggctgag ctcgtcatat tcatatagtt tatagtcttc
    11161 tatcagggca atgagcctgt cagcatactg cggattggtg gcatacccgg ctgccttcag
    11221 ccctttggcc cagcctttgt aatcattggg atccagttcg aacaaacctg cataacgctg
    11281 ccgggtaagc aggaacatgg aatggtcacg ataggactct tccggattgg aatattttct
    11341 gaaacattct cctttggcat catcatcatg atagactctt tcaccatccc acccatcatg
    11401 gcatttgatg ccgaagtgat tattggattc agaggcaagc tgactgcgtc cggctcctga
    11461 ttccagtatg ccctgcgcca gggtaatgct ggcaggtatg ccatggttga tcatttcttc
    11521 taccgcgata taccggtagc gttcaatgta ggccagtacc gcctcattcg gatcgccggg
    11581 aaacagtgag aactgccccg tcagcagggt aaatgccaac agtgtattct tcatagtatt
    11641 gcaggcatag aagcccattc aaaactactt gtagaaacgc ctggggcatt tggctattat
    11701 ccataaaaaa atgtgcttta tccatttttc cgaacaatca tgatgccatc acgaacagaa
    11761 agcagcacat tttctacccg ttcatcggcc tgcacgagtt cattgaatgc tctgatggcc
    11821 gtagtgtcct tgtctgtggc ttcttccacc acttttccgc tccatagtac attatctgcc
    11881 aggatccatc cgcccgcagg caccttatct atgaccagtt catagtatct ggaatagttg
    11941 cgtttgtctg catcaatgaa aacgaggtca aatggtccgg aaagttccgg gatgatccgg
    12001 gtggcatctc cggtccggat atcgaatttt tctgtgagtc ctgctgcatc cacatagcga
    12061 cgcaccatgg agctgagttc ttcgttgatg tctatggtgg tgatcgtccc tccttccacc
    12121 agtcctttcg ccatacagat gccgctgtaa cccgtgtagg tgccgatctc caggatgcgt
    12181 ttcggcctga tcatcgtgct gatgaattcc aggaattttc cctgcactgc tccgctcagc
    12241 atgcgtggct ggagtacttt caggtgggtt tcgcggtcca gctgctgcag gtgttcgtcc
    12301 ggtttgctgc tgtgctgttc tatatatgca tcgagtgatg ggggcaggat ataggtcatg
    12361 atctgacaat actataacat aaagcagata aactatgcat acttttattc ttaaacagat
    12421 catatgcgtt tcgttttact tttaaccatt tctttgctgt cattcacggt atcagcacaa
    12481 ctgaacatta catgggtgtc atcattgcct tattctcagc tcagcagctt atggggatac
    12541 actgctcctg acaatacgga gtatgcattg gtaggggcat acaacggcct ttctattgtg
    12601 agtctggctg accctgctgc ccctgaagaa ctattctttg tagaaggtgc agaaagtgcc
    12661 tggcgagaag tcaaaacatt cggccattat gcctatgtga ccaatgagac cggtggcggc
    12721 ctgctgatcg tagatctgca ggatctgccc ggcgccattg atacggtctc atatgcaggt
    12781 acagcagatc atccctttac tacggctcat acactttttg ttgatgagaa cggcatactt
    12841 tatctgttcg gatacaatgt ggtctttgga ctcacaggag cctatatgct ggacctgaac
    12901 actgatccaa tgaacccgga ctttgtggct gtgtatgaag accactatat acatgatgcg
    12961 tttgttcgca atgataccat gtgggccgct gagatctatg acggtcggtt cgaggtattg
    13021 gatatcagtg ataaaacatc tttcctgtcg ctgggatatg ctgagaccta tggagtggcc
    13081 acgcacaatt gtgagccgga tgccagtggc aatatccttt tcactactga tgagatattc
    13141 aacggatata tcacttcttt tgatatcagt gatctgactg acatccagga actggaccgg
    13201 tataagcatg gtgatacggc tctggccata ccacacaatg tacgggtact gggtgatttt
    13261 ctggtagctt ctcattacag tgagggggtt gtcattctcg atgcttcccg ccccggcctg
    13321 ctggtagaaa ccggtcacta tgataccaat cctgctggtc ctgatgcatt ctttgaaggt
    13381 gcctgggagg tttaccctta tttgccatcc ggtttgctga tcgtttctga tattctggag
    13441 gggttgcagg ttctgcaacc ggagtatgtt tatgctgcct ttctggaagg tgttgtgacc
    13501 gatgaagtta ccggggcatc catcccattg gctacaattg aattgaccgg taccactgca
    13561 acggcacagg ctgacctgtt gggtcagttt gaattgggct atcattctgc cggcatctat
    13621 gatgtgagtg tttctgctcc cggatgcagc accagggtct ttgctgatgt agaactggaa
    13681 aacggtgtca ctgctatact ggatgctgct ctggactgcg gtacagttgc actgccagcg
    13741 gtacccgatt gtagcggatg gctggccggg actgaccaca gcaccggatc agtatggatc
    13801 cagaactact gtacagagag taataccacc tgcaccatga ccgacctgaa tggcagaatg
    13861 atctggtctg gaagcgtatc aggtacagat cgttatcaga ttcctgttac cacaccaccc
    13921 ggcatttact ttcttgctga tcagttgggc cagacacgtt ccgttacggt catgcattaa
    13981 gattgcataa aagttgctgc ctgcatctgt attcggggca atttctcttt tctttgcact
    14041 atgaaaatga gatggcttct tctgctttgc ctctacgccg gaatggtaca ggcacagctg
    14101 ctgccatcct atggagattc acgcaccgct accaccggct ggcagtttct gaaagtggtg
    14161 cccgatgccc gatctgcagg aatgggtgag gccttcctcg ctgtggcaga tgatatggga
    14221 tccgtgtact ggaatgcagc aggtctgagt aagctggata ccagtcgctg gcacaccatg
    14281 ctggcacata ctgcatatcc tgcttccatc aatcagtatt acggagcact ggcctggcga
    14341 ctgaactcca acacattatt tgcaggaagc ctgcagtatt ttgacgccgg ttccatgccg
    14401 gtcaccacag aatttcagcc cgccggaaac ggcatgacct ttcacccggt caatatggct
    14461 ctgggcgcca gctggtcgca ggtactgacc gatgcattca gtttcggtgt gaccctgaaa
    14521 tatgtaaggg aagatattgc tgacgtgcac aaccagaaca tgctgttcga tctgggcttc
    14581 cggtatgata tcggtattgc ccggacccgt tttgccgtgg cactcaccaa cttcgggttc
    14641 aatacaacgc ccgatggttc catacctgta ctgtccatta ccgataccac cgtcatcgtg
    14701 caggccgaag atgttggggt tcccaccatt tttcgtcttg gatttgcctg ggatgcactg
    14761 gcttcagaga cccatcaact caccgctgca gcccagctca accatcccac agataacaat
    14821 gagacctatt ctatcggact ggaatacggc tggaagaaac tgctttttgt gcgaagcggg
    14881 tatcagttcg ccactgacct agcagcctgg ccctcgttcg gattcggtgt cagggtaccg
    14941 cgttattttg gtacggtaca actggattac ggatttcaat atatgaacag actgggaacg
    15001 gtcaaccgca tcggtctgca attatctcta tgaaaaaact ggcttacatc ctcatctgtt
    15061 ttcctctgct gagcagttgc gattttttgt ttggtacaac aaaagatgat gtagccgcag
    15121 agatctttga agaaggcgcc atcgatccgg aactcattcc ttcagaagtg ggctatgtac
    15181 cagtgctgcc attctggaca ggctttgatc atccggtaga tatatactgc ggttatgatg
    15241 agatgatcta tgtgatagat gacaacgggc tgaacgtgct ggatcaggcg ggaaatctgc
    15301 atgacatcat ttacattcag ggtgcctcag atgtatgtca ggaccgtagg ctgcatacct
    15361 atgtgaccgg gcgggtggat gtggatgtgg atgacgacgg aatgcccgaa aacctggctg
    15421 cggtatatca cctgacgggt acggctaccg gaaatgtaca gttcattgat acccttattc
    15481 atccgttctg cgatgaaagc cgcaacatca cttctttccg tggtacagat gatatcgcag
    15541 ttgaattcac cggtgtgacc actctggccg ataataccct gcacgtgtca cgcaccggtc
    15601 cacgcaatga cctgacaggt atcgctagag tggataatac tattctgttc tttgatgagg
    15661 atggcatcaa caccggctat tccaacggac tgagtccggt atcatccaac ctccgatcat
    15721 cctggaatat ctcttctatc gccggatttg ctgctccacc ccaatcgctc gcaggaatca
    15781 gcacctcggc tgattttctt atcaccctgg aggcggaaga tgcagcgtac aaagtgttgt
    15841 ggatccagca gaatgtggat ccggaagcgg gagtcaccta tggagagaat gcagcactgg
    15901 cagcatttga cctgaccaaa gccgatcgtt tcctctacga acccggcaga tttgaagctc
    15961 ctgcagatgt gtatgtggca cctgatttta ccggttacat atttgtggtg gacagtgagc
    16021 tggactctct gtatcagttc acccgcaggg gctatgaagg tgtgaaccca ccaccgggaa
    16081 gcaccacaac aaaacagatc atcgccagct ttggcggtgc cggcgatggc cccttcaact
    16141 tcaacgaacc cagtggtgtg gcttatctgc gcagggtggt ctatgtggca gataagggca
    16201 acgggcgcat ttgccgttat acgctgagta ccgacctcga atgatctttc tcctaccttt
    16261 gtgcgtatga gcaaacagat agttgtactg ggtgccggca tggtaggacg tgccatggcc
    16321 attgacctgg caaaggattt tcaggtgacc agtgccgata taagccggga gaatctggac
    16381 ctgcttctgc cctttggtat ccataccatt caggctgatc tgtctgatgc tgctgccatt
    16441 caggaattga tcgctgctgc agaccttgtg ataggagcgg tacccggatt catggcattc
    16501 agaaccctgc agaccgttat tgaagcaggg aagaacatat gtgatatttc cttttttgat
    16561 gaggaccctt ttctgctgga tcagcttgca aaagacaaag gtgtaactgc catcatggat
    16621 gtgggtgtgg cacccggaat ggacaatatc ctgctgggat atcaccatcg gcggatgcag
    16681 gtgaaccggt tcgtttgtct ggtgggcgga cttcccaaac accgacagtg gccatatgag
    16741 tataaggctc ccttttcccc catcgatgta ctggaagaat atacccgccc ggcccgatat
    16801 gtgcaggatg gaaaggaagt ggtcatgcct gcgctcagcg aaccggagct catggagttt
    16861 gcagaagtgg gtaccctcga agctttcaac agcgacggac tgcgcagcct gatacgcacc
    16921 atgcccgata ttccgaacat gattgaaaga actttgcgct atccgggtca tatcgaactg
    16981 atgcgggtat tgcgggagac agggttcttc agtaaagaag ccatactggt gaaagggcag
    17041 gcaatccgac cgatagacct gacctcgcaa ctgctgttcc cgatgtggaa actagagcag
    17101 gaagaagaag aattcactgt gatgcgggtc atcatagagg gaacagaaaa cggttcacat
    17161 cgcacctata cctatgacct gctggatcgt tatgatgccg caacgaagac ttccagcatg
    17221 gcacgcacca ctggctatac ctgcacaggg gctgcccgcc tggtgttgaa tggcatgtat
    17281 gataagaaag gggtctttcc gccggaattc ctgggtgaag aggaggcaca cttgtctttc
    17341 atgctggaac atttggctca acgaaacata cattaccggc aacagatcag ctgatcaggt
    17401 tttcacagat atgaccgaaa gcgagtatat tcggtttacc atgaagcgcc tgttgtcagt
    17461 actttgtttc ttactttttt attgcgccgc ccttcgggca caacttcctg atctttattt
    17521 tgaacagatc actacccgga atggactgtc caacaacagc ataacctgct tgtttgaaga
    17581 cagccggggc ttcatctgga taggtacccg ctttggcctg aaccggtatg atggccagac
    17641 ttatgagcat tttttgaaat caaatcagaa caggaattcc atttccggca actatatagt
    17701 tcgtgtactg gaagatcagg atgggatcat ttgggtggct acaaaagatg gcggccttaa
    17761 ccgctttgat gagcaggcaa aggaaggttt caggtggacc gcactcatgg aaagcactga
    17821 taacaagacc gggttgcctt caaaccggct tacagatatt gtcatgctaa acgactccgt
    17881 tttgttgctg acagcagagg tcaatccctt gatcctgttg cacacaagga acatggagtt
    17941 tagatacatc gctcctgcta agaatgtgac tgatgctgta ctgtccgttc gatgggatga
    18001 tgcaggatct gaaatcaagg atcctttttg gatacatagg ttcaaaactt tcagagggca
    18061 gattttcctg tctcccttaa aacctggtcc ggtagccgca atagatgcta ctggcaagct
    18121 ggtgttgagg ttccgtgatc tgccatgtac caccatcgca gaatttttac cggaaggaga
    18181 tagtctctgg gtcagtggat ggaaaggcgg tttgtttctg caaagaaact ccggcactgg
    18241 agatgatccg gtgatcacag aagtttttga tgcggaagat cagatcaatt gtatcgaatc
    18301 attggatgat catacgctgt tgttgggaat gaacagtacc ggagtggcac tgcttgataa
    18361 gcggacgatg aagtccagat ttttcatgtc agaagtgaac aatgcatatt ccattcccgg
    18421 taacagggta ctggcaatga tgatagatcg caatggcatc gtctggatcg gaacaacaaa
    18481 ggggctggcc agatacaatc cggcccagtg gaagattcat gctgagttac ttccgtatga
    18541 cgaagccaga gacatcagtt attattcagt cttcacagag tcatcaggca ctctgcgtat
    18601 ctgctcaagc gcagggatat tcaaaaaaga accggcagat gctttcttca gaagggtaga
    18661 attagacttt aaaggccagg ccatccaggt aacaggtatt gtacagttgt ctgatggcag
    18721 attcatgcta tcatcagagc agggtttgtt ctggtatgac cctgtgaaag agcagttgtc
    18781 tgactggata ccgtctttag tgtacctgag agacactgtg atcatcaaca gatcacatta
    18841 tagcaggatt gcctttcagg tgagaagccc ggtcgtttat aaggatcagg acaaggagtt
    18901 cgtactggca gcagttatcg gatatggttt acttgtatat gatgtcagtg ataatgttaa
    18961 ttattttctg gtaagggatg aaaagaatcc tttatcattt ggagacggcc tgctgctaaa
    19021 tgtagctatt gataaagatc gtcaggtatg gttgggcagt actcagggtt tatacaggtg
    19081 gcgtagtgaa gaagggcttg tgaacaactt cgaccgcttt ctgcacgatc cggatgaccc
    19141 ggcaagcata ccttcaaatt atatcacatc catcatctct gatcctgaaa aaggtgtctg
    19201 gatcaccaca cctgatggat tgggtaggtt ggtagatgga agctgccaga cctggctgcc
    19261 aggcaatgac ctgcaccgtg tcatgctggg aggtctgtac gatcagggaa aggtgtgggc
    19321 tgctgtattg gaagggttcg agatatttga tgtaaaacgg aaaacattca gataccaggg
    19381 tattccccat gaatcctggt acccaagtgc accactgggg atcatcaagt taccagacgg
    19441 ccagtttgcc tacgcaagcg gaaagcattt tctgaccttt catcaggatg atctgattgc
    19501 agatcgcagt ttccctgatg tgtttattaa aacattgctg gttcaggact ctctgatcta
    19561 tctgaaggaa tatcctgatc gtattgtcct taagcacaac cagaatttcg ttcagttcaa
    19621 tttggctggt attcaggttc gatatccggg ttcactgaag atcaggtaca ggctaactgg
    19681 acttgaggag aattggacag aatcatctgt cacatcattt atcagattta caaatctgac
    19741 tccgggcagc tacgccctcg aagtacaggt gaccaatccg gtaggtgaat ggagtgagac
    19801 gaatacatta tacctgttgg acatttcaaa accctggtat ggaacctggt ggttttacgg
    19861 cttgtgtctg gtcatgtcac tatctgtttt caggctgatc atcttcctga gagagagaag
    19921 cagatatcag atcgagatga tcagaaacag gatagcaaac gatctgcatg atgatgtggg
    19981 aagtgcattg agttctattc atctgtacag cgaagtggca cataatcact cacagaagaa
    20041 ccaacaggaa ctgcggagct tgttacagaa gatctctcat gccagccttg aaatgcagga
    20101 gaatatggga catattattt ggagtctgca gcccagaaat gcccctgccg ggcagatggg
    20161 agccagatta aggcaactgg caggcgagat gcttggcgcc gccaatatca gattcactta
    20221 cgaatgggat gataacctgg gcagcttgca tttatcacca gtgcagcgca aagaatgcta
    20281 tcttatctgt aaagaggcgc ttcataacat catcaagtat gcagcctgca ccaaagtgac
    20341 catccgtctt taccgtaaat cagaatcgat gtgtgttgat atcagtgata atggcaaagg
    20401 atttgacaag gaatcggttc agtatggaaa tggattgtat tcgatgcaac aacgggcaga
    20461 atcactcaaa ggatcactga aaataacaag ccagccaggc gacggtgtgt tggtcagctt
    20521 atgttttccg gccggaaccc gcaagatctg gtagaagatc agaaatatga atgaattaat
    20581 ttaggatcat gaaggagatc agggtagctc tgtttgacga taatgacatc cggcgggatg
    20641 gagtggagtt attgctgaaa actaccgagg gatatgtctg ctgcggatcc tatgaacatg
    20701 ccggcaacct cgtggcagag ctccatgatg cacagcccga tgtggtcatc atggatattg
    20761 atatgccggg aattgacgga attgaagcgg tcaggatact gaagggggct ttccccaacc
    20821 tcccggttat gatgcagact gtctttgatc atgatgagaa agtttttgaa agcatcctgg
    20881 caggagccac aggttatttg ttaaagaaaa caccaccggt caagttactg gaggccatca
    20941 gtgagatgca tgacggaggt gcgccaatga caccagagat agccgccaag gtcctggcct
    21001 tttttgcaaa acagaacacc ccggaacc
//
LOCUS       DAOMAX010000014        18978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_296_length_18978_cov_4.789198, whole genome shotgun sequence.
ACCESSION   DAOMAX010000014 DAOMAX010000000
VERSION     DAOMAX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18978
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_18978_cov_4.789198"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /gene="crtI"
                     /locus_tag="PK742_01800"
     CDS             <1..1256
                     /gene="crtI"
                     /locus_tag="PK742_01800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HQU75351.1"
                     /translation="LDPGYRVFFGKDDIRDIPASLDDLYALFEAEEPGSARHLKKFLD
                     DAAYKYKVGMQDLVFKPCHSVMEFADKRLLSSLFRMQLFTSLEREVRSKFKSEKLRRL
                     LEFPVYFLGALPSNTPALYSILNYADLVLGTWYPMGGMGKIVEGMQRVAENMGVQFRF
                     NSPATGFDLGDDRVKAVLTADGPVVCDGVIASADYQFVEQQLLPADKRNYSAAYWNKK
                     VMAPSCLIYYLGVDGTIDGLKHHNLFFDESFDDFAEAIYTNPKWPENPLFYTCIPTKT
                     DASVAPDGKENLFILIPVAPGLEDTDEIRSRYFEKAMDKLEKHTGQQIRHRVEVQHSF
                     AYRDFVDRYHSFRGNAYGLANTLMQTAVFKPKMRNKHLNNLLYAGQLTVPGPGVPPSL
                     VSGNVAAGEMQKMLFAEQKIEQIAV"
     gene            1286..2122
                     /locus_tag="PK742_01805"
     CDS             1286..2122
                     /locus_tag="PK742_01805"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HQU75352.1"
                     /translation="MMQLFHDSSFACSKKITELYSTSFTLGIRALHPDFRDAIFGIYG
                     FVRYADEIVDTFHDHDKKYLLDSFREDTWKALDGGISLNPVLHAFQLTVNKYQIDRKL
                     IEAFLHSMEMDLYFADYKREHYDEYIYGSAEVVGLMCLKVFCNGEEEQYNQLVPAARS
                     LGAAFQKVNFLRDMKSDYQDRGRVYFPQVDFSTFSEEMKRQIEEEIAADFEKAYEGIR
                     QLPARSRFGVYLAYRYYLALFRKIRTTPAERVLEARIRVSNPRKLALYASSYLQHSVI
                     GV"
     gene            2122..2820
                     /locus_tag="PK742_01810"
     CDS             2122..2820
                     /locus_tag="PK742_01810"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HQU75353.1"
                     /translation="MDRSYTYLLINLLSVAMPLAFSFEPRVRFAGWWKYLFPAMLITA
                     APFLAWDAFFTIEGVWGFNPDYLTGWHLGVLPVEEILFFLAIPYASIFIYAAWNWWKP
                     ESPLMDRMAKPITWFFILLALAGLVFFYDRWYTAVNCAFALVLLLVQQFLVKGSYMGR
                     FWRFYLVHLIPFFLVNGILTGSWIKAPVVWYNNAENTGIRIGTVPIEDSMYSLSLMLM
                     NIMLIERWKKPAVI"
     gene            2817..3272
                     /locus_tag="PK742_01815"
     CDS             2817..3272
                     /locus_tag="PK742_01815"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQU75354.1"
                     /translation="MTWLINIASLLGAFIFMEGFAWFMHKYVMHGFGWFLHKSHHEPR
                     HGRFEWNDLYAVIFAAPAIYFMWLGSPEYNYIFFAGCGITLYGLCYFLFHDILVHRRM
                     NHHVRPGNRYMQRIIRAHKIHHKNMRKENGKAFGFLFALPEYDVKRTDE"
     gene            complement(3275..4405)
                     /locus_tag="PK742_01820"
     CDS             complement(3275..4405)
                     /locus_tag="PK742_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU75355.1"
                     /translation="MWQIAKKDLYIFFADKKALFLTLLLPIGLITLFAFAFGGVGQSA
                     SEPNPVILQYSDLDNSPASAALIARLDSISGIRMEAVDTAAGMENIRKGNRIALLVID
                     QGLEPALAQQSALPAHLFFDESQAMEVSILQNMLRGMLGQMRATAAPAPESAGHQEPE
                     ASNLQMVSVVGDETSNWGLIQAVAGTAIMMLLFSVSAIGASIIEEKEQGVLRRLLQSP
                     QPAFSLLNGKLIFATIIALFQLVVMFLFAWMAFGLPVGMNLPGLILMIVVTALCCSSF
                     GVLLASVADSKRQADSLGTIIILFMSAIGGSMIPLYIMPGFMQDIAVISVNYWSIQGF
                     YDIFWRQLGIEALTENVLILLGYTVVLLTLSYYFFRRKALRL"
     gene            complement(4412..5329)
                     /locus_tag="PK742_01825"
     CDS             complement(4412..5329)
                     /locus_tag="PK742_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU75356.1"
                     /translation="MLNVNHIRKRFGQLQALEDVSFNIRKGTCYGLLGPNGAGKSTTI
                     HSISGILAPDSGSILIDGTDLYSHTAACKRKMGVVPQEIALYEDLSAWNNLLFWGSLY
                     DMSKQDTLKRAAILLDWVGLADRKNDLIAHYSGGMKRRINIAAALMHDPDLILMDEPT
                     VGIDPQSRNHIYTMLQDLRKEGKTILYTTHYMQEAEELCDEIGIIDNGQVIASGTLEE
                     LRHQHPIEESLHVHFSESVTTLTDLPWTHQWEQDHKKLIISSRQTHKDLAAVIQFLDS
                     RQISPEQIKFIPINLETIFLHLTGRTLRD"
     gene            5407..6111
                     /locus_tag="PK742_01830"
     CDS             5407..6111
                     /locus_tag="PK742_01830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU75357.1"
                     /translation="MQAGIWQEQEWESVFFGFRVARIFPPFTFQQATDALLSLTAADF
                     SFLYGFVDPASPEQHILEQLGGKVIATRATYSMPVSDIQTPSVARIATQNDLPELEIL
                     ALQSGLYSRYRKDVMFPEMMYEKMYRTWVRNTLEKTFGDEVLVIEEDGTLAGMVTMSK
                     SGQLATVGLFVVLNIFRRQGIGKGLLDAAMEWSRQQGCKELRVITQLENRPACRFYSG
                     RGFELLQVENAVHLWL"
     gene            6102..6344
                     /locus_tag="PK742_01835"
     CDS             6102..6344
                     /locus_tag="PK742_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HQU75358.1"
                     /translation="MVVEDLPVYTRQQLALRNGQDREEVWIAMDGLIYNVSASRLWFR
                     GTHYEHWSGQDLTDELPDAPHTKEVLQRFPVVGRLA"
     gene            complement(6341..7255)
                     /locus_tag="PK742_01840"
     CDS             complement(6341..7255)
                     /locus_tag="PK742_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQU75359.1"
                     /translation="MSLLVVGTVAFDAIETPFQKTEKIIGGSGTYIGLSASFFVKPIQ
                     LVSIVGSDFPQETLKYFNRKGIKTEGVEVKKGKKSFFWSGKYHMDMNSRDTLITDLNV
                     LAEFDPVLPQSYQSPDYVMLGNIDPNLQIKVIEQLRNRPKLIAMDTMNFWMNTTPNEL
                     AKALKLTDVLIINDEEARQLSGEYALAKAAKVIMKKGPRILVIKKGEHGALLFQGDEV
                     FFAPALPLEEVFDPTGAGDVFAGGFMGYLSSSNNRSFTNMKRAVVYGSAMASFCVEKF
                     GPQRLTEIRKDHIQKRVGQFVDLAKFRI"
     assembly_gap    7355..7454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7610..7683
                     /locus_tag="PK742_01845"
     tRNA            7610..7683
                     /locus_tag="PK742_01845"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7644..7646,aa:Asn,seq:gtt)
     gene            complement(8080..9795)
                     /locus_tag="PK742_01850"
     CDS             complement(8080..9795)
                     /locus_tag="PK742_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75360.1"
                     /translation="MKKILLSLSILISAYSYGQISGTSVFLQGQYVEVGINGCGVYGS
                     HEEPPVGPYGAYHVANANGLGYVADSEMDGWDVHTDPDAPVFCGDYFTPGSPEEGWAI
                     QYGDNVYENHSTYCSGYGEYAYLDSTGNIPGSNTFYSDSAGMLTAIWEGSLVDGDLDL
                     SVKQITTLADTALFFISTIEITNNGATDLTDLYYIRNVDPDQDLDHCGTFATYNSILS
                     NTPASDTVIVTAIGSSCGCYFGTTAVDPRARVSYGAFFLSPNTPFDAYYGLGDVMPYY
                     LEGEDYWCDCSVQITYKFDLPAGATTSLSYARMFGESATENAQQALIDLNEPVISADG
                     AAVVSDGSVMLCEGQSMELSVDGPAGYTWTWEPADYLDSGVGSNVISTPSADITYTVT
                     GTDGTNTVNTSVSIQISEAIVLNMESTPSVDGAPTGTATASIEAGGLEPFTYEWSNGA
                     TTPVIAGLDPGTYIVWVTDAAGCTATQSIVVSLANDLQSVDASGIYSLFPNPATDQLN
                     LELADWVTNAIIEVVTVNGGEVMLNQSVQEQLSTFDVSTWHPGVYLVKVCTAAGCFGQ
                     TILVQ"
     gene            9882..10583
                     /locus_tag="PK742_01855"
     CDS             9882..10583
                     /locus_tag="PK742_01855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQU75361.1"
                     /translation="MEESRILLEDPWFLVMFKPSGLQVEPDKNGHPNLMEQVEAIYRA
                     KGTLPGNFMLRVPHRIDRPTSGLQIISLRKAALLHFMQAFEQRDIVKEYLAVVEGEAP
                     EAAMLMHGLRKDPVHFRAEWSDRAGNGFKEARLKTERLGVDNNRSLLRLTPETGRYHQ
                     IRAQLSAIGHPVINDPLYGAVRVDDTARIGLLASLLRFRHPRTQELITCTTDPGEETW
                     WDPFRQTIAEISKPL"
     gene            10609..13668
                     /locus_tag="PK742_01860"
     CDS             10609..13668
                     /locus_tag="PK742_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU75362.1"
                     /translation="MLRKTLPGLLLLCGVFSAKAQLVISSDTTLCETAPVTLEVISAP
                     SYGTTSYTFVEADYAPEAYAGTTISLSDDSYGGPYSIGFTFCFLGNEYTQFYVGSNGW
                     LSFGGAGALTTTFTSAPIPSVAGTVPKNCIMGPWADWHPGLCAGCIKYQTIGTAPNRK
                     LVLSFDNVPMYSCTTTYGKFQIVLHETTNIIENHITNKDYCAWAGGTATQGVHNLSGT
                     VAFTVPGRNSTVWTADDETVQFVPSGITWYSGATIIGYGDSIVVTPGVTTTYTAEVES
                     CDGTVYTADVTVAIELDDPTFTYADTEFCPDDVITPTYIATPGGTFVIDPPDMPVNAA
                     TGQINRSGGEPGTTYTVEYTTPPGICSSSSGFSFTIVPYDDATVSYASPSFCPTGTAS
                     PTVATPGGTYTIAPAGMSINPTTGVLTLSTGTVGTTYTVTYTTAGFCPSVATTTVLID
                     PLDDPTFAYDSASYCPSGFALPTVVTGGGTFTASPGSLTIDATTGEINLSTGIPGTTY
                     TITYTTPAGSCSNFSTETVTINYLDVASFGYSASSYCAGGTVSPTFITTPGGSFTVTP
                     SGLDVDPVSGDVDLTTGTVGITYAITYTTDSGPCSNSGTVSITIDPNDDSTFYYTAPS
                     FCAAGSAVPVVVTPGGNFVSAPAGLSIDPASGTVDLSSSTPGTYTITYTTPAGFCSSS
                     MSQTLTVTDFTDAFFEYDAVEYCNEGSVMPFILNPGGTFSAPGSVSIDISTGEIDLGA
                     SVAGGPYQILYNSPGCTEQDTFFLTIHPMPSLTIDLPDLTCYEDDPILLSGTPAGGFF
                     SGTGVTGNIFLPGAVPAEGLYPVTYTYTNEFGCTSELTEYIQVILHTVSAGPDQTIIE
                     GQYAVLEASDGVIFTWDPADWLGCSGCQSTSATPPLGTMNYLLTSYDENGCVAFDMVN
                     VTVIPFDDVTVFVPNTFTPNGDHINDYLMPYGSDVAGIISFTVYDRWGEIVWQRGNIA
                     PDHTDGGWNGTSLNGQPLNAGVYAYAIEVYLSFGVTKTVYGNVTLIR"
     gene            13702..15144
                     /locus_tag="PK742_01865"
     CDS             13702..15144
                     /locus_tag="PK742_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQU75363.1"
                     /translation="MIRIQNYINGQYVPPVSGQYMDNFEPATGKVYGEIPLSGKEDVE
                     SAIRAAGMAFPAWSKTGIRERSRILLRIADLIMEKLDELALAESTDNGKPVSLATEVD
                     IPRAAQNFEFFATAIQQFASESHANPGKNVHYTLRQPLGIVTCISPWNLPLYLFTWKI
                     APALAAGNCVIAKPSEVTPMSAYLLAGICMEAGLPPGVLNILHGTGPDCGAAMVAHPD
                     VKAISFTGSTRAGREIASVAAPQLKKLSLELGGKNPVLIFDDCDYDLMLQETVRAAFS
                     NQGQICLCGSRLYIQDTIYEKFREDFVEKIRQMRVGDPRSVDTNIGAVVSEAHLGKIF
                     EYLRIAAEEGGRVLCGGERVTPEGRCAEGYFVQPAVVEGLPNDCRTNQEEIFGPVVTI
                     QPFHSEEEVLAMANDSSYGLASVIWTRHLSRAHRLSETMQSGLVWVNCWMERDLRTPF
                     GGMKNSGVGREGGWEALRFFTEPKNVTIQY"
     gene            15155..16180
                     /locus_tag="PK742_01870"
     CDS             15155..16180
                     /locus_tag="PK742_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQU75364.1"
                     /translation="MQRLKILLPINTIAVASLLFSSCGLMEQAMRSTPGADEQLVQEE
                     PVFIEDFYEADIAEAGKTVSIVEPIQRKDAFITPPAEQIAAREMVYDLDEIAGISDIG
                     GGEEISYEDEEASEVALSTAMVGEQFNPSKLEGKVMVDVLMDDVSCYTFPLDQLRINS
                     NFGWRRGRVHSGIDLDLEIGDHVYAAFDGTVKRAEYTKGYGNLIVLEHPNGLQTYYAH
                     LSKIKVEPGQEITSGDLIGLGGSTGRSTGPHLHFEVRYLGAYMDPSNLIDFKTGELKS
                     TILTVQKSSFNLQNDVSTAKYHTVRSGDTLSKIARQYGTSVSRICKLNGISSTKVIRP
                     GQRLRVR"
     gene            16249..17121
                     /locus_tag="PK742_01875"
     CDS             16249..17121
                     /locus_tag="PK742_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQU75365.1"
                     /translation="MPLQAQTENIGKEVEQSAELMGNQWDTFTTLLPNILIALCVIIL
                     FILLSRFIRKIFYKLLLKRLSNPAVTTITASFISIIFVFIGIFLALDIVGLDKTVSSL
                     LAGAGILGLALGLALQDTLTNTVAGIVMTTRKAYKVGDYVESNGFEGTILEINLRNTT
                     ILQNSGTQVKIPNRNVLNNPLQNFSLTGERRVEVKVGVHYREKLEVVEKITLEAIRNS
                     VDFNERRPLELYFTDFGDSSINLIVRFWIKKVRQADFYKAKSDAIKAIRNAYRDHNIT
                     IPFPIRTIEMERGN"
     gene            17152..17571
                     /locus_tag="PK742_01880"
     CDS             17152..17571
                     /locus_tag="PK742_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HQU75366.1"
                     /translation="MSQDIINTGNAPAPVGLYPHARKAGNLLFLSGIGPREAGSDRIP
                     GLELDARGNFVHFDFEAQVHSVFKNVKEVLEASGSSWDKLIDVTVFLVHMQRDFQTYN
                     RIWAEYFPENAPCRTTVSITALPTPIAIELKCIAMLD"
     gene            17550..18404
                     /gene="kdsA"
                     /locus_tag="PK742_01885"
     CDS             17550..18404
                     /gene="kdsA"
                     /locus_tag="PK742_01885"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQU75367.1"
                     /translation="MHSHAGLMEKQLETYSGTVQVSDGISLPAARYLLLSGPCVIESQ
                     QLCLDVAGVMQEICRDLGIDYVFKASFDKANRTAASSFRGDGMDAGLQVLEAVRKTGV
                     PVVTDIHLPEQAAEVATVADILQIPAFLCRQTDLLLAAGLTGRAINIKKGQFMAPEDM
                     AYAADKVRSTGNMNVLLTERGSSFGYHNLVVDMRSLPVMRQFAPVLFDVTHSVQMPGG
                     AGGKSGGDRRFAPYLARAAAAAGVDGFFMETHPDPDHALSDGPNMIPLDQMRSLLEVL
                     LQIRKASE"
     gene            18401..18886
                     /locus_tag="PK742_01890"
     CDS             18401..18886
                     /locus_tag="PK742_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQU75368.1"
                     /translation="MSLDYSPVKLVLSDVDGVLTDGGLYFTESGDQFKKFHVHDGMGF
                     LLLKKAGIKTGIITSENLELVRLRAQRIQPDHLYMGVGFEEKLHTARAIARQEGISLD
                     EVAYIGDDVNCIALLEKVGFPTCPADAVDAVKNIPGIFQTTRKGGEGAFRELADKILS
                     F"
BASE COUNT         4783 a         4477 c         4693 g         4925 t
ORIGIN      
        1 gacttgatcc gggttatcgg gttttttttg ggaaggatga tatcagagat atccccgcct
       61 ctctcgatga cctgtatgcc ttgttcgagg cagaagaacc cggtagtgcc agacacctga
      121 aaaagtttct ggatgatgcc gcatacaaat acaaggtagg catgcaggat cttgtattta
      181 aaccctgcca ttcggtcatg gagttcgcag acaagcgcct gctcagttct ctttttcgta
      241 tgcagttgtt cacctcgctg gaaagggaag tgcgtagcaa attcaagagt gaaaaactgc
      301 gtcgtctgct ggaatttcct gtttattttc tcggtgcgct gccgtccaac actcctgcgt
      361 tgtactctat tctcaactat gctgacctgg tacttggcac ctggtatccg atgggaggga
      421 tgggaaagat cgttgagggg atgcaacgtg tggccgaaaa tatgggcgtg cagttcaggt
      481 tcaattcgcc ggcaacagga tttgacctgg gtgacgatag ggtgaaggcg gttcttacag
      541 ccgatggtcc tgttgtgtgc gatggagtca ttgcttctgc cgattaccag ttcgtggaac
      601 aacaactgct gcctgctgat aaaagaaact acagcgctgc atactggaat aagaaggtca
      661 tggcacccag ttgtctgatc tactacctgg gtgttgacgg aaccatcgac gggctcaaac
      721 accacaacct gttctttgat gagtcttttg atgattttgc cgaagcgatc tataccaatc
      781 cgaagtggcc ggaaaatccc ctgttctata cctgcatccc caccaaaaca gatgcatcgg
      841 tagcacctga cggtaaagag aatcttttta ttctgatacc tgtggcaccg ggcctggaag
      901 acacggatga gatcagaagc aggtattttg aaaaggccat ggacaagctg gaaaaacata
      961 ccggtcagca gatccgccac agggtagaag tacagcatag ttttgcttac cgcgactttg
     1021 ttgatcgcta tcattctttc cgcggaaacg cctatgggct tgccaatacc ctgatgcaga
     1081 cagcagtatt caagccgaag atgcgtaaca agcacctgaa caatctgcta tacgccggcc
     1141 agctgaccgt accgggcccc ggagttccgc ccagcctggt gtcaggaaat gtggcagccg
     1201 gagagatgca gaaaatgctg tttgcagagc aaaagattga acaaatagcc gtttaaacgg
     1261 tttattcacc ctaacttcct cccttatgat gcaactcttc cacgactcat cctttgcctg
     1321 cagcaaaaag atcacagagc tgtacagcac ctcttttacc ctgggtattc gtgcactcca
     1381 tcctgacttt cgcgatgcca tttttggcat ctatggcttc gtaagatatg cagatgagat
     1441 cgttgatacc tttcatgatc atgataagaa gtatctgctg gactcgttca gagaagatac
     1501 ctggaaggca ctggatggag gcatatcgct gaatcctgtt ctgcatgcct ttcagctgac
     1561 ggtcaataaa tatcagatcg accgcaagct gattgaagca tttctgcaca gtatggaaat
     1621 ggacctctac tttgcagact ataagcgtga gcattatgat gaatacatct atggttcagc
     1681 agaggtggtt ggccttatgt gcctgaaagt tttctgcaat ggagaagaag agcaatacaa
     1741 tcaactggta cctgcagcac gaagtctcgg agcagctttt cagaaggtga acttcctccg
     1801 cgatatgaag agtgattacc aggatcgtgg aagggtgtat ttccctcagg tagatttttc
     1861 cactttcagt gaagaaatga aacgccagat tgaagaagag atcgctgcag actttgagaa
     1921 agcttatgag ggtatcagac aacttcccgc aagatcaaga ttcggggttt acctggctta
     1981 ccgctattat cttgccttgt tcagaaagat cagaaccacg ccagctgaaa gggtgctgga
     2041 agcccgcatc agagtaagta acccaagaaa gctggcattg tacgccagtt cttatcttca
     2101 gcattctgtt atcggggtgt aatggaccgg tcttacactt acctgcttat aaacctgctt
     2161 tctgtagcca tgcccctggc tttcagtttc gagcctcgtg tgcgttttgc aggttggtgg
     2221 aagtacctgt ttccggccat gttgatcacc gctgcacctt ttcttgcctg ggatgcattc
     2281 tttaccatag aaggggtatg ggggttcaat cccgactatc tgaccgggtg gcacttgggt
     2341 gtattaccgg tggaagagat cctctttttt ctggccattc cgtatgccag tatattcatc
     2401 tatgctgcat ggaactggtg gaaaccggaa agtcccctca tggacagaat ggccaaaccc
     2461 ataacatggt tctttatcct gttagcattg gccggactgg tattttttta cgaccgatgg
     2521 tacactgcag ttaattgtgc atttgcactg gtgttgctgc tggttcagca atttcttgta
     2581 aaaggcagtt atatgggcag gttctggcga ttttacctgg ttcatctgat accctttttt
     2641 ctggtgaacg gaatactgac cggaagctgg atcaaagcac ctgttgtctg gtataataat
     2701 gctgaaaaca ccggtattcg aatcggtact gttccaattg aagattctat gtattcattg
     2761 tcgctcatgc tcatgaacat catgcttatt gagcgatgga aaaaacctgc agttatatga
     2821 cctggttgat caatattgca tctttattag gcgcattcat ttttatggaa ggttttgctt
     2881 ggttcatgca taagtatgtg atgcatggct ttggatggtt cctccacaag tctcatcatg
     2941 aacccaggca cggacgtttt gaatggaatg acctgtatgc ggtcatcttt gcagcacctg
     3001 ctatctactt tatgtggctc ggttcgcctg aatacaatta tatcttcttt gccggctgtg
     3061 gcattaccct gtacgggttg tgttattttc tctttcatga tatcctggtg cacagacgca
     3121 tgaaccatca tgtcagacca ggtaacagat acatgcagcg catcataagg gcacataaga
     3181 tacaccacaa gaacatgcgg aaggaaaacg gcaaagcatt tggttttctt tttgccttac
     3241 cggaatatga tgtgaagcga actgacgaat gaccttacag ccgcagggcc tttctgcgga
     3301 agaagtaata agacaaggtc agcagtacga ccgtatatcc aagcaatatc agcacatttt
     3361 cagtcagcgc ttctattcct aattgtctcc agaagatgtc ataaaagccc tggatgctcc
     3421 agtagttgac agaaataaca gcaatgtctt gcatgaaccc tggcatgata tacagtggaa
     3481 tcatgctgcc tccaatagca gacatgaaaa ggatgatgat cgtgcccagg ctgtctgcct
     3541 gtcttttgct gtcagcaaca gaagccagca gtacaccaaa ggatgaacag catagtgcag
     3601 ttacaacgat cataagtatc aagcctggca gattcatgcc aacaggcaag ccgaacgcca
     3661 tccaggcaaa cagaaacatg accacgagct gaaaaagtgc aatgatcgtg gcgaagatca
     3721 gttttccatt cagcaggctg aatgcaggtt gagggctttg cagtaacctg cgcaacaccc
     3781 cttgttcttt ttcttcaatg atgctggctc caatggctga aacggaaaac aacaacatca
     3841 tgatggccgt gcctgctaca gcctggataa gcccccagtt acttgtttca tctccaacta
     3901 cactgaccat ttgaagattg cttgcttcag gttcctggtg gcctgctgat tcaggagccg
     3961 gagctgcagt agcccgcatt tgccctaaca tgccacgcaa catattttgt aatatactaa
     4021 cctccatggc ctgtgactca tcaaaaaaca gatgcgcagg cagtgcgctc tgttgtgcca
     4081 aagccggttc cagtccttgg tctatgacca gcaaggcaat tcggtttccc ttccgaatgt
     4141 tttccatgcc ggctgcagta tcaacagctt ccatacgaat gcctgatatg ctgtccagcc
     4201 gggcaatgag tgcagcagaa gcagggctgt tgtccagatc tgaatattgc agaataaccg
     4261 ggttgggttc tgaggcgctt tggcccaccc cgccaaatgc aaatgcaaac agcgtgatca
     4321 gtccaattgg caacaaaagt gtcagaaaga gggctttctt atctgcaaag aatatgtaca
     4381 ggtctttttt agctatttgc cacatggtct ttcagtctct cagcgttctg ccggtaaggt
     4441 gaagaaaaat tgtttccaga ttgataggta tgaatttgat ctgctcgggt gagatctgcc
     4501 tgctgtcaag aaactgtatg actgcggcca gatccttatg tgtttgccgg ctgctgatga
     4561 tcagtttttt atggtcttgt tcccattgat gtgtccaggg caggtctgtc agtgtggtaa
     4621 cagattcaga aaaatgaaca tgcagtgact cttcaatagg atgctgatgg cgcagctcct
     4681 ccagtgtgcc gctggcaatt acctggccgt tgtctatgat gccaatttca tcacagagtt
     4741 cttcggcctc ctgcatataa tgggttgtat agaggatggt cttgccctct ttacgcaggt
     4801 cttgtaacat ggtatagata tggttgcggc tttgtgggtc aatgccaact gttggttcat
     4861 ccataaggat cagatctggg tcatgcatca atgcagctgc aatattgata cgccgtttca
     4921 taccacctga ataatgtgct atcaggtcat tctttctgtc agccagtcca acccaatcaa
     4981 gcaatattgc tgcacgcttg agggtatcct gtttggacat gtcatagagg ctgccccaga
     5041 acaacaaatt attccaggca ctcaggtctt catacaaggc gatctcctgt ggcactactc
     5101 ccatctttcg tttgcatgct gctgtatgag aatagagatc agtgccatcg atcagaatgc
     5161 ttccgctgtc tggagcaagg ataccggaga tcgagtgaat tgtggttgat tttccggcgc
     5221 cattgggacc aagtaatcca taacaggttc cctttcggat gttaaaactt acgtcttcga
     5281 gggcctgtag ctggccaaat cgtttcctga tatggttgac atttagcata ctgaagtgat
     5341 tgcagcggaa caacttcccg cttagaatgg ctaaatttat aacctaagca gctaacagaa
     5401 gaaaaaatgc aggcaggcat ctggcaggaa caggagtggg agagcgtttt ttttggcttc
     5461 cgtgtagcca ggattttccc tccgttcact tttcagcaag ctacagatgc attgctcagc
     5521 ctgactgccg ctgatttcag tttcctgtac ggatttgttg atcctgctag ccctgaacag
     5581 catatattgg aacaactggg cggaaaggtc attgccacca gagctaccta ttccatgcct
     5641 gtttctgaca tacaaacacc atctgttgct cggatcgcaa cgcaaaatga tcttccggaa
     5701 ttagagatac tggccttgca gagcgggttg tattcacggt acaggaaaga tgttatgttc
     5761 cccgaaatga tgtatgagaa gatgtacaga acctgggtca ggaatacact ggagaaaact
     5821 tttggagatg aagtactggt catcgaagaa gatggcactc tggctggtat ggtaaccatg
     5881 tctaagtcag gacaactggc aactgttggc ctgtttgtgg tactgaatat tttccggcgg
     5941 caaggtattg gaaaagggtt actggatgct gccatggaat ggagccggca acaagggtgt
     6001 aaggaactcc gtgtcatcac acagctggag aacagacccg cctgcagatt ttactcaggg
     6061 cgtggattcg aattgttaca agtagaaaat gcagttcacc tatggttgta gaagatctgc
     6121 cagtttatac aaggcaacaa ctggcattga gaaacggaca ggaccgggag gaggtgtgga
     6181 ttgcaatgga cggactgatc tacaatgtga gtgcaagccg tttgtggttt cgcggcaccc
     6241 actatgaaca ctggagcgga caggatctga ctgatgaact accggatgcc ccgcatacta
     6301 aagaagtact gcaaagattt ccagtagtgg gcaggcttgc ttagatcctg aattttgcca
     6361 gatcaacaaa ctgaccaacc cgtttttgaa tatgatcctt cctgatctct gtgagccttt
     6421 gaggaccgaa tttctcgaca cagaatgatg ccatcgctga gccatagaca actgcccgtt
     6481 tcatattggt gaaggagcgg ttgttactgg aagacagata tcccatgaag ccaccggcaa
     6541 agacatcgcc ggctccggtc gggtcaaaca cttcttcaag cggcagtgca ggcgcaaaga
     6601 acacctcatc gccctggaac aacagggcac cgtgttcgcc tttttttatt acaaggatac
     6661 gaggtccttt cttcatgatc actttagcag ctttagccag ggcatattca ccacttagtt
     6721 gacgggcttc ttcgtcgttg atgatgagta catcggtcag cttcagggct ttggccagtt
     6781 cattcggcgt agtattcatc cagaagttca tggtatccat ggcgatcagc ttgggcctgt
     6841 tgcgaagttg ctcaatgacc tttatctgga gatttggatc aatgttaccg agcatcacat
     6901 agtccggcga ctgataagac tgtggaagca ccggatcgaa ctctgccagt acattcaggt
     6961 cggttatcag ggtatctctg gagttcatgt ccatgtggta tttgccgctc cagaagaatg
     7021 atttctttcc ctttttgacc tcaactcctt ctgttttgat gcctttgcgg ttgaaatatt
     7081 tcagggtttc ctgcggaaaa tcgctcccca caatggaaac cagctgaata ggtttgacaa
     7141 aaaaggacgc tgataatcca atataggtac cggatccgcc gatgatcttt tcggtcttct
     7201 gaaaaggggt ttcaatggcg tcaaaggcta cagtaccaac cacgagcaga ctcatagaaa
     7261 aaaattttcc gcaaagattg tataaaattg gctgtaaggc tacatttgca gtccgttttt
     7321 cggtaaacgg aaagcaaaat tcctccgtag ctcannnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnngctggt tagagtcccg cacatgcggg attaatcaga gggtcgatgg
     7501 gaacaaaaag acaggagata acactcctcc gtagctcagc tggttagagt cccgcacatg
     7561 cgggattaat cagagggtcg atgggaacaa aaaagacagg agataacact cctccgtagc
     7621 tcagctggtt agagcatctg actgttaatc agagggtcct tggttcaagt ccaagcgggg
     7681 gagcaacaca cgaggccaga gagatctggc ctcgttatgt tttatatcta catactctac
     7741 ggagcgtcat ctgacaaata ctacgtcgga tattctcagg atccatggta tcgactggag
     7801 cagcataata ctgcatggac caccaagtat acaggtaagt tgggagagtg gatgcttgct
     7861 gcggtttttg aggcaggagc aagcagaagc gaagcgatga ggctggaaaa atttattaag
     7921 cgtcaaaaaa gtcgacggtt gatggaacga ctgaccgacc ccgactttgt cccgtccgga
     7981 gaattggctc agctggttag agtcccgcac gcacaggatg aatcatcata aaaaaagggg
     8041 aagcgtcgct tcccctttgt gctatatgtt ttaatgtgct tactgcacca gtatggtctg
     8101 accgaagcaa ccggctgcag tacaaacttt taccagatat acgccgggat gccaggtgga
     8161 aacatcaaat gtgctgagtt gttcctgaac gctttgattc agcatgacct caccaccatt
     8221 taccgtgacc acctcaatga tagcgttggt cacccagtct gccagttcca gattcaattg
     8281 atcagtggca ggattcggga acagggagta gattcctgag gcatctacag attgcaggtc
     8341 attggccagg ctgaccacta tactttgtgt tgcagtacaa ccggcggcat ctgttaccca
     8401 aacgatgtag gtgcctggat ccagaccagc aatgacaggt gtggttgctc cgtttgacca
     8461 ctcatatgta aatggttcta aacctcctgc ttcgatagaa gcggtggctg tacctgttgg
     8521 tgcaccatct acactgggag tactttccat attgagcaca atagcttcgc tgatctgaat
     8581 acttacgctg gtgttgacgg tattggttcc gtctgtaccg gttacggtgt aggtgatatc
     8641 agctgaaggt gtgctgatca cgttcgatcc gactcctgaa tccagataat cagccggttc
     8701 ccaggtccag gtataacctg ccgggccatc tacagacagt tccatgctct gaccttcaca
     8761 aagcatgaca gaaccatcgc ttacaaccgc tgcaccgtct gcactgatca caggttcatt
     8821 caggtcgatc aaggcttgtt gggcattttc ggtagcagat tcaccaaaca tcctcgcata
     8881 gctcaggctg gtagttgcac ctgctggcag atcgaatttg taggtgatct gaacggaaca
     8941 atcacaccag taatcttcac cttccagata gtatggcatt acgtctccta atccataata
     9001 ggcatcaaat ggtgtattgg gtgacagaaa aaaagcgcca tagcttactc tggctctggg
     9061 gtcaacagcg gtggtgccaa aataacatcc acaggaagat ccgatggctg taacgatgac
     9121 cgtatcagaa gccggtgtat tactaagaat ggaattatag gtggcaaatg tgccgcaatg
     9181 atcaagatcc tgatcaggat ccacattccg gatatagtac aggtctgtca ggtcagttgc
     9241 gccattgttg gtgatctcaa tagtggagat gaaaaacaaa gcagtgtctg ccagtgtggt
     9301 gatctgtttg acagaaagat ccagatctcc gtccaccagt gagccttccc atatggcagt
     9361 gagcatgccg gcactgtcac tgtaaaaagt gttgcttccc ggaatgtttc cagttgaatc
     9421 gagataggca tattctccgt atcctgaaca ataggtgctg tgattttcat atacgttatc
     9481 gccgtactga atggcccagc cttcctccgg tgaaccgggg gtgaaataat cgccgcaaaa
     9541 aacaggagca tccggatctg tgtgcacatc ccagccatcc atttcagaat cagctacata
     9601 gcccaaaccg tttgcgttgg ctacatggta ggctccatat ggcccgacag gaggctcttc
     9661 atggctgccg tacacgccac aaccattgat gccaacttct acatactggc cttgcaggaa
     9721 tacagatgtt ccgcttatct ggccataact gtatgctgaa ataagaatag acagggagag
     9781 gagaattttt ttcatatcag gtatttattc tttctaaaga tactgaatag attataaaaa
     9841 aatattcctg ggtatgccgc tttgaaatgg taatcttgtg catggaagaa agccggatcc
     9901 ttttggaaga cccctggttt ctggtcatgt tcaaaccatc cggtttgcag gtggagccag
     9961 ataagaacgg tcatcccaac ctgatggaac aagtagaggc catctacaga gccaaaggta
    10021 ctttacccgg taacttcatg ttgcgggtgc cgcatcgtat tgacaggcct acttccggtt
    10081 tgcagatcat ttcgctaaga aaagcggccc ttctgcactt tatgcaggct tttgagcagc
    10141 gtgatatagt aaaagagtac ctggctgtgg tggaagggga agctccggaa gcggccatgc
    10201 taatgcatgg tttgaggaaa gatccggtgc atttcagagc tgaatggagc gatcgtgccg
    10261 gaaacggatt caaggaagca cggctgaaaa cagaacgttt gggagtggat aataatcggt
    10321 ccttgttgcg attaacgccg gaaacaggcc gctatcacca gattcgggca caattgtctg
    10381 ctattggtca tccggttatc aacgatccgc tatacggtgc tgtcagggta gatgataccg
    10441 ccaggatcgg actgctggca tctttattac gatttcgcca cccccgaact caggagctga
    10501 tcacgtgtac cacagatccg ggtgaggaaa catggtggga tcccttccgg caaacgattg
    10561 ccgaaatctc gaaacctttg taatttcgaa ccacacattc taccaggcat gctgcggaaa
    10621 acccttcccg gcctgctgct gctttgcgga gttttctctg caaaagcgca gctggtcata
    10681 tcttctgata ccaccttgtg tgaaacagca ccggttacgc tggaggtcat ctctgccccg
    10741 agttatggta ctaccagcta tacatttgtg gaagctgact atgcgccgga agcttatgcc
    10801 ggtaccacga tctcgttaag cgacgattca tacggtggac cctattccat tggatttacc
    10861 ttttgttttc tgggaaatga atacacgcag ttctatgtag gttccaatgg ctggctcagt
    10921 ttcgggggcg ccggagcact cactactact tttacttctg ctcccattcc aagcgttgca
    10981 ggaactgttc cgaagaattg tatcatggga ccctgggcag actggcatcc cgggttgtgc
    11041 gcaggctgta tcaagtacca gaccattggt actgcaccca accgcaagct ggtgctgagt
    11101 tttgacaatg tgcccatgta ttcctgcacc accacctacg gcaaatttca gatcgtgctg
    11161 catgagacca ccaatattat tgaaaatcat attacaaata aggattactg tgcatgggcc
    11221 ggaggtactg ctacccaggg agtacataac ctgtcaggta ccgtggcctt cacagttccg
    11281 ggcagaaact caacggtttg gactgcagat gatgagaccg ttcagtttgt tccttccggt
    11341 atcacctggt acagtggtgc aaccatcatt ggttatggcg acagtattgt tgtgaccccc
    11401 ggggttacca ccacctacac cgccgaagta gaatcctgtg atgggactgt ctatacggca
    11461 gatgttactg tagctattga attggatgac cccactttta cttatgctga cacagagttt
    11521 tgtccggacg atgtgatcac cccaacttac attgcaactc cgggaggaac ttttgtaata
    11581 gatcctccgg atatgcctgt caacgctgct accgggcaga tcaaccggtc aggcggtgaa
    11641 ccaggaacta cctacacagt agaatacacc accccaccag gtatttgttc ttcatcatcc
    11701 ggtttcagtt ttactatagt tccttacgat gatgccactg tcagttatgc atcgccgtca
    11761 ttctgtccaa caggtacagc atctcctaca gttgccactc caggtggaac ctatactatt
    11821 gctcctgccg gtatgtccat aaaccccacc accggagtac tgacactttc cacaggaaca
    11881 gttggaacaa cctacacagt cacttataca acagccggtt tttgcccttc tgtggctacc
    11941 accacggtct taatagaccc tttggatgac cccacctttg cctatgactc tgccagctat
    12001 tgtccatccg gttttgccct gccaaccgta gtaacgggcg gaggtacatt tactgcctca
    12061 cccggctctc ttaccatcga tgccaccacc ggcgagatca acctgagtac cggtattccg
    12121 ggaactacat ataccattac atacaccaca ccggcagggt catgcagtaa tttcagtacg
    12181 gaaacagtta ccatcaatta cctggatgtt gccagctttg gatattcagc ttcgtcctat
    12241 tgtgccggtg gtacggtcag ccccaccttt attaccacac caggaggtag ctttacagtc
    12301 actccatcag gacttgatgt ggatccggta agcggagatg tggaccttac aaccggtaca
    12361 gtaggcatca cctatgccat cacttatact acagactcag gcccctgctc taattccggt
    12421 acggtcagta ttactattga tcccaatgac gactctactt tctactatac agcaccatcc
    12481 ttctgcgccg ctggttcagc agtacctgtt gtagttacac cgggtggtaa ctttgtgtct
    12541 gctccggcag ggctgagtat cgatcctgcc tcgggtacgg ttgacctgag ttccagcaca
    12601 cccggaacat acaccatcac ttacactact cctgctggat tctgcagttc cagtatgtct
    12661 caaacactta ctgtaactga ctttacagat gcattttttg aatatgatgc tgttgagtat
    12721 tgcaatgagg ggtcggttat gccctttatt ctgaatcccg gaggcacttt ttctgccccc
    12781 ggatctgtca gcattgatat cagtaccggg gagatcgatc tgggtgcttc agtggcaggc
    12841 ggtccttatc agatactgta caattcgccg ggttgtacag aacaggatac tttctttctg
    12901 accatccatc ccatgccatc actgacaatt gatttgccag atctgacctg ttatgaagat
    12961 gatccgatcc tgctgagtgg cacacctgcc ggtggctttt tttccggaac tggtgttact
    13021 ggaaacatct ttctgccggg ggcagttccg gctgaaggtc tgtatcccgt tacctacacc
    13081 tacaccaatg aatttggctg tacatctgag ttgacagaat acattcaggt tatccttcac
    13141 acggttagcg caggacctga tcaaaccata attgaagggc agtatgcggt gctggaagcc
    13201 agcgatggag tgatctttac ctgggaccct gctgactggc tgggatgcag cggatgccag
    13261 agcacttctg caaccccacc tctgggaacc atgaattacc tccttacctc ctatgatgaa
    13321 aacggttgtg tggcatttga tatggtcaat gttacggtca ttccttttga tgacgttacg
    13381 gtctttgttc cgaatacatt tactcctaac ggcgatcaca tcaatgacta cctgatgcct
    13441 tacgggtctg acgtggccgg aatcatatca tttaccgtgt atgatcgctg gggagagatc
    13501 gtctggcagc gtggaaacat agcacctgat cataccgatg gcggatggaa cggcacctca
    13561 ctcaacgggc agccccttaa tgccggagtg tatgcctatg ccattgaggt ttatctcagt
    13621 ttcggggtga cgaagacagt atatggtaac gtgaccctta tccgatgatt ggttactttt
    13681 gtagcctcat tttcaggaag tttgatccgc atccagaatt atattaatgg tcagtatgtg
    13741 cctcccgtat cggggcaata catggacaat tttgaaccgg caaccggaaa ggtttacgga
    13801 gagatcccat tatccggcaa ggaagatgtg gaatctgcca tcagagcagc cggtatggca
    13861 tttcctgcct ggagtaaaac cggcatacga gagcgttcca ggatcctgct gcgcatagca
    13921 gacctgatca tggaaaagct ggatgaactg gcattggctg aatctacaga caacggtaaa
    13981 cccgtcagtc tggcaaccga agtagatatt ccaagggcag ctcagaattt tgagtttttt
    14041 gcaaccgcca tacaacagtt tgcctcagaa tcacatgcca acccgggaaa gaatgtgcac
    14101 tataccctga gacagcctct gggaattgtt acctgtatct ctccctggaa tctgcccctg
    14161 tatctcttca cctggaagat cgcaccggca ctggcagcag gcaattgtgt gatcgcaaaa
    14221 ccatcggagg ttacacccat gtctgcctat ttgctggcag gcatctgcat ggaagccggt
    14281 cttccgccgg gtgtgctgaa tatcctgcat ggtaccggac ccgattgcgg agcagccatg
    14341 gtagcccatc ctgatgtcaa ggctatttca tttaccggta gcacacgtgc aggaagagag
    14401 atcgcttctg ttgctgcgcc tcagctgaag aaactcagtc tggaactggg aggcaagaat
    14461 cctgttctga tctttgatga ttgtgactat gatctcatgc tgcaggaaac tgttagggca
    14521 gcattcagca accagggtca gatctgttta tgcggctcca gactgtatat tcaggatacg
    14581 atctatgaaa agttcaggga agattttgtc gaaaaaataa gacaaatgcg tgtgggagat
    14641 cccaggagcg tggatacaaa tattggcgct gtggtcagtg aggcgcatct cgggaagatt
    14701 tttgagtatc ttcgcatagc cgctgaggaa ggaggacgtg tgctttgtgg tggcgaacgg
    14761 gttacaccgg aaggccgttg tgccgagggg tattttgttc agccggcggt tgtggaaggg
    14821 cttccgaacg attgtcgaac caatcaggaa gagattttcg gtccggtagt gaccattcaa
    14881 cctttccatt ctgaagaaga agtgctggca atggccaatg attcttctta cgggctggcg
    14941 tcagttatct ggacccggca tctttccagg gcacatcggt tgtccgagac catgcaaagc
    15001 ggattggtct gggtcaattg ctggatggaa agagatctta gaacgccgtt cggtggaatg
    15061 aaaaattccg gcgtgggcag agaagggggg tgggaagcac ttcgcttctt caccgaaccg
    15121 aagaacgtaa cgattcaata ttaaacagga acacgtgcag agattgaaga tcctcctccc
    15181 catcaacacc attgccgttg catccctttt gttcagttca tgcgggctga tggaacaggc
    15241 catgcgaagt actccgggtg ctgatgaaca gctggttcag gaagagcctg tttttataga
    15301 agatttctat gaagccgata ttgcagaagc aggtaaaaca gtttccatcg tagagcctat
    15361 tcaacgtaag gatgccttca ttacccctcc ggcagagcag atagcagccc gagaaatggt
    15421 gtatgacctg gacgaaattg ccggtatcag cgatattggt ggcggagaag agatcagcta
    15481 tgaagatgaa gaagccagtg aagtggcact ctctactgcc atggttggag aacagttcaa
    15541 tccgtccaaa ctggaaggca aagtgatggt ggatgtactt atggatgatg tttcctgcta
    15601 tacctttccg ctcgatcagc tgcgcatcaa ttccaacttc ggctggcgtc gcggacgggt
    15661 acactcaggt attgaccttg acctcgagat cggcgatcat gtctatgctg cctttgacgg
    15721 aaccgtgaaa cgggccgaat acaccaaagg ttatggtaac ctgatcgttc tggaacatcc
    15781 caatggatta cagacctatt acgctcactt gtcaaagatc aaagttgaac cgggacagga
    15841 gatcacctcc ggcgatttga tcggcctggg aggcagcacc gggcgctcta caggccccca
    15901 cctgcatttc gaagtacgct atctcggtgc gtatatggat ccgtctaatc tgatcgactt
    15961 taagaccggt gagctcaaga gtaccattct gaccgtacag aaaagttctt tcaacctgca
    16021 gaacgacgtc agtacagcca agtatcatac agtgcgttct ggtgataccc tgagcaagat
    16081 cgccagacaa tacggcactt cagttagccg catatgtaag ctgaacggca tctcttcaac
    16141 caaggtcatt agaccgggcc agcgcctgcg tgttcgctga ttcggatcta cagctgattt
    16201 atagaatcag gttgcggcct ttacaccgct caggcagtat atttgaccat gccacttcag
    16261 gcacagacag aaaatatcgg aaaggaagtt gagcaatcgg cagaattgat gggtaaccaa
    16321 tgggatactt tcactacgtt attacccaac attcttatcg ctctttgtgt cattatactg
    16381 ttcattcttc tctccaggtt catccgcaag atcttttata aactgctgct gaagaggctc
    16441 agcaatccgg cggtcaccac catcacagcc agtttcatct ctatcatttt tgtattcatt
    16501 gggatctttc ttgccctgga tatagttggc ctggataaga cggtcagctc tttgctggca
    16561 ggagccggaa ttctcggtct ggcacttggt ctggctttgc aggataccct taccaatacg
    16621 gtagcgggca ttgttatgac cacacgcaag gcctataaag tgggagacta tgtagagtca
    16681 aacgggtttg aaggaaccat actggagatc aacctgcgca ataccaccat cctgcagaac
    16741 agcggtacgc aggtgaagat cccgaacagg aatgtgctga acaaccccct tcagaatttt
    16801 tcccttacag gtgaaagaag agtggaagtg aaggtggggg tgcactatcg cgaaaaactg
    16861 gaagtagtgg agaagatcac cctggaggct attcgcaaca gcgtagattt caatgagcga
    16921 aggccactgg aactgtactt cactgatttt ggcgacagca gcattaacct gatcgttcgt
    16981 ttctggataa aaaaggtcag gcaggctgac ttctacaagg cgaaaagtga tgccatcaag
    17041 gccatcagga atgcataccg tgatcataac atcaccattc cattcccgat caggaccatc
    17101 gaaatggaac gcggcaactg atgcttttct taaactgctt acctttatac catgagtcag
    17161 gacatcatca ataccggaaa tgcaccggct ccggttgggc tctatcccca tgccaggaaa
    17221 gccggtaacc tgctctttct gagtggtatt ggtccgcggg aagcaggctc agaccgcatt
    17281 cccggactgg agctggatgc cagaggaaat tttgtgcatt tcgactttga agctcaggtg
    17341 cacagtgttt tcaagaatgt caaagaagtg ctggaagcca gtggcagcag ctgggataag
    17401 ctgatagacg tgaccgtttt cctggttcat atgcaacgtg attttcagac ctataatcgc
    17461 atatgggccg aatactttcc ggaaaatgca ccctgcagaa ccaccgtttc cattacggct
    17521 ttgcctactc ctatcgctat tgaactgaaa tgcatagcca tgctggactg atggagaaac
    17581 aactggaaac atattcggga actgtacagg tgagtgatgg catcagcctg cccgctgcac
    17641 ggtatctgtt gctttccggc ccctgtgtca ttgaaagcca acagctttgc ctggatgtgg
    17701 ctggtgtcat gcaggagatc tgtcgtgatc tgggtattga ctatgtgttc aaagcttctt
    17761 ttgataaggc taacagaact gcagcctctt ctttcagggg tgatggaatg gatgccggac
    17821 tacaggtact cgaggcagtc agaaaaaccg gcgtgcctgt tgtgacggat atccatttgc
    17881 ccgaacaagc cgctgaagtt gccaccgtgg cagacatact gcaaatacct gcttttctgt
    17941 gcaggcaaac tgatcttctg ctggcagcag gtcttacagg aagagcgatc aatatcaaga
    18001 aaggacagtt catggcaccg gaagacatgg cttatgcggc tgataaggta cgctccactg
    18061 gtaacatgaa cgtattgctc accgaaagag gcagctcctt tggctaccac aacctggtag
    18121 tggacatgcg ctctctccct gtcatgagac aatttgcccc ggtattgttc gatgtgaccc
    18181 acagtgtgca gatgcctggc ggagccgggg gtaaaagcgg tggcgacagg cgctttgcac
    18241 cttatcttgc aagagccgct gctgctgccg gagtggacgg tttcttcatg gagacccacc
    18301 ctgatccgga tcatgcactg agcgacggcc cgaatatgat accgcttgat caaatgagat
    18361 ctctcctgga agtattgttg cagatcagaa aagcaagcga atgagcctgg attattcacc
    18421 ggtgaaactg gttctctcag atgtggatgg tgtgctcacc gatggcggac tatacttcac
    18481 tgaatccggc gatcagttca aaaaattcca tgtacatgat ggcatgggtt tcctgttatt
    18541 gaagaaagct ggtatcaaaa ccggcatcat cacctctgaa aatctggaac tggtcagact
    18601 gagggctcaa agaattcaac ccgatcactt gtacatgggc gtaggctttg aagaaaaatt
    18661 gcatactgct cgggccatcg ccagacagga aggcatttcg ttggatgaag tggcctatat
    18721 aggtgacgat gtgaactgta tcgccttgct ggaaaaagta ggcttcccca cctgtcccgc
    18781 agatgcggtg gacgccgtca aaaatattcc gggaatcttt caaacgactc gaaaaggcgg
    18841 cgaaggggct tttcgcgaac tggcagacaa gatcctgtct ttctgaactt gtaccatttt
    18901 tttagtcacc ttgcgggtcc tatatcctgg cactgtataa gaatttgtgc agtcttgtga
    18961 cgtgtgttct gtggacat
//
LOCUS       DAOMAX010000015        18961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_298_length_18961_cov_4.254893, whole genome shotgun sequence.
ACCESSION   DAOMAX010000015 DAOMAX010000000
VERSION     DAOMAX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18961
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_298_length_18961_cov_4.254893"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PK742_01895"
     CDS             <1..378
                     /locus_tag="PK742_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQU75369.1"
                     /translation="MVRAVQEGHAMNRKGVLFWLGLTIIGGLIFVGSQAFEWSHLIHE
                     GMTQKSNPFGASAFGGLFFLITGFHGFHVSVGVMLNSWAFLMTFFGAFHRRGHYEMIE
                     KLGLYWHFVDLVWVFVFLAFYLL"
     gene            392..718
                     /locus_tag="PK742_01900"
     CDS             392..718
                     /locus_tag="PK742_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQU75370.1"
                     /translation="MSNLTHEESIKKVWRVCGILAFVTIFEIAAALFHFYSIPESPRW
                     WLNLLFIILSAAKAYYIMSEFMHLKYELKAMTLTVMTPFLFLIWGIIAFLWEGGYWLS
                     LREFWQ"
     gene            721..1353
                     /locus_tag="PK742_01905"
     CDS             721..1353
                     /locus_tag="PK742_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75371.1"
                     /translation="MQQQRSWFWMVVMAILLIVLPLISYLLVRQGALIRQHAAASEYI
                     KELPGGLPPDQLFISQRGDTLTAERLLGKVVVVECINSTSAGKTGERHPLFEIQEAYE
                     GKTLNLRFMSVWEDTGRAATTADLAYFGSRFAVRENWHVVSCGQPESLLSSIDENFYN
                     GEKKSGDSDLFFPHVVYVLNKEGHWCATYKLDDQQAYDALFNDLLYLIDR"
     gene            1350..2000
                     /locus_tag="PK742_01910"
     CDS             1350..2000
                     /locus_tag="PK742_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQU75372.1"
                     /translation="MKRNTWILIIVVGVAFAAFFKWVYLPMAARQDQISRMADDLYPT
                     DKLRQPSATIPPFSMTDQNGQTITEQDVKGHIHIADFFFTSCDAICPMMTSQLSRVQE
                     AMGDERDYRILSFSLDSENDSVPVLRSFARKFGANDTVWHLLTGDKKAIYTLGAEGYK
                     QSVLRDAENDINHSGRLILVDRNLQIRGFYNALDSLEVDMLINDLSYLLYKEEWNE"
     gene            1993..2508
                     /locus_tag="PK742_01915"
     CDS             1993..2508
                     /locus_tag="PK742_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HQU75373.1"
                     /translation="MSERLQNRLIWIISIAVPAVVAMLFFMPGLDLGTDVSMLPGLNA
                     VINGTVSVLLIAGYILIRSGKRQAHRAAMLSAFFLSALFLISYVIYHSATESTSFGGA
                     GLIRTVYFFILITHIVLAAAILPLILITMSRALSSRFDRHRKIARITFPLWLYVSVTG
                     VVVYLMIAPYY"
     gene            2510..2764
                     /locus_tag="PK742_01920"
     CDS             2510..2764
                     /locus_tag="PK742_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75374.1"
                     /translation="MKVRMLFFLVMLFLLIGVSSADAQCAMCKMAAENGAQADGKPYL
                     ALNQGILYLAAVPYLSFSVIAFLFWRGYRRRREQEAELEA"
     gene            complement(2765..3466)
                     /locus_tag="PK742_01925"
     CDS             complement(2765..3466)
                     /locus_tag="PK742_01925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU75375.1"
                     /translation="MRAVITGASKGIGAAIAEKLASEGCDVFLIARSEDHLKRTCSSI
                     LSAGNTVSCSYHTADLSDISQAQASGKIVLETFGVPDILINNAGQFIPGSLYNEPPGT
                     METMMNTNFYSAYHLTRELIPGMMSGKSGHIINICSIASIQAYPDGGAYSVSKFALLG
                     FSKNLREELKPHGIKVTSVLPGATYTDSWASSGLPAARFMQPEDIANVVWQCIALSET
                     ATVEEIILRPQLGDI"
     gene            3514..5961
                     /gene="priA"
                     /locus_tag="PK742_01930"
     CDS             3514..5961
                     /gene="priA"
                     /locus_tag="PK742_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQU75376.1"
                     /translation="MNTLYADIILPFAVPGTFTYEVPEAGRELAIAGMRCEVHFRKKI
                     QAGIILRIHQEKPSGFQPRQIIQFPDDEPVVSTLQLRLWIWMSTYYMCTLGEIYQAAV
                     PALFKSGSETQISLNQMLDTDTLDLDDRSFILVEALKHQKVMQLKDVQELLGIKQIQS
                     LLRKLVMNNVILLQEEVRDVYKPKVRKLIRLTERFQDEEALRQLFDELNRAVRQQHVL
                     MIYLQLAPEGEAVSRAVLLKKAEASAAVLKAMTDKGIFEETEEVVSRLKLSGNSSLTR
                     SKLSLTQQEALHSIRSQWQEKEVVLLHGVTGSGKTEIYMELIEEVLERGKQVLYLLPE
                     IALTAQIIGRLQERFGDQVGIYHSRFNHQERVEIWQKVQQGSYRILLGARSALFLSFQ
                     DLGLVVIDEEHDYSFKQQDPAPRYHARDTAIYLASMVAAKVLLGSATPSVEAYQHAHT
                     GKFGYVELTERFAGMELPEIELINMQSETQQNNVVTHFSRKLLAAMEEAVAGKEQVIL
                     FQNRRGYAPYVVCTSCGWTPVCVRCDVKLVYHKFSGELRCHYCGYKQEIVTACPECAS
                     TQIIVQGFGTEKVEDELQALYPAWRIARLDLDAVRTKAGHEKVIHAFEDGQVDVLIGT
                     QMVTKGLDFDNVRLVGILSADQLLQFADFRASERAYQLMMQVSGRAGRKQKRGKVLIQ
                     TLRLDHPVLGFVQRHETHTFYEEEIRQRRNFKYPPFNRIIRITLRHAEPGLSLNAAYG
                     LVNALGNGPAFEVLGPVQPAIARIRGKYLSEVMIKVRNDGQSAKAAKQRLRQAMERVA
                     AKKEWKKVEIIPDIDPV"
     gene            complement(5962..7386)
                     /locus_tag="PK742_01935"
     CDS             complement(5962..7386)
                     /locus_tag="PK742_01935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQU75377.1"
                     /translation="MRRRKKIGLITYLFFDLVAAALAWCVTFAFRKFFIEDRPFDLQE
                     YVFSDPKFYLGAAAVACGWILIYFVAGFYTDIYRKSRLAEFYRTFLAAFLGSMVLFFV
                     LILDDYIEGYQDYYQTFLILAGSQFLLTTGSRMTILTLAKRQLQSGQIAYNTLLIGGN
                     RRALDLYMEVTRKKKSLGYSFIGYIDANGQNGNELSKYIPHLGGLDQLDDILLHRSID
                     EVIIAIETSEHHLLNDIMNRLSDLRGVVVKIIPDMYDIMSGSVKMSNVFDAVLIEIYP
                     DLMPKWQRTIKRMLDVVISIFVFCILWPLYLFIAIKVRLSSSGPVMYKQTRIGINGKP
                     FTIYKFRSMFQDAEKTGPALSSEEDQRITSWGKIMRKWRFDELPQFYNILLGDMSLVG
                     PRPERKYYIDQIVQRAPEYKHLQKVKPGLTSLGMVKFGYASNVDEMVERLKYDLLYIE
                     NMSLAMDFKIMFYTLVIIFQGKGK"
     gene            complement(7389..8231)
                     /locus_tag="PK742_01940"
     CDS             complement(7389..8231)
                     /locus_tag="PK742_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75378.1"
                     /translation="MKRHLQYLLMIPVLVSACTGDELQPAFVHIADFEVISDPGSEGS
                     PTQKITDAWVYVNGIQLGVFELPVTVPVLDTGTVSISVFAGIKENGFSSIGNIYPFYS
                     GFETTLTLRPFETDSIQPSVDYLSGLSWIYLERFELGNSFTQLSGSETGLEIVTDPEL
                     VLEGSRSAFANMEPGISVMKVTTGPLLFPTVGNAVWAEVDYKASVPFEVWVNGNYTGG
                     IPISSYMLTVGARNDWNKIYLNLAAKVATLQAETYNLEFRCYKPDSVSSAELYFDNIK
                     IIAF"
     gene            complement(8228..9211)
                     /locus_tag="PK742_01945"
     CDS             complement(8228..9211)
                     /locus_tag="PK742_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQU75379.1"
                     /translation="MVRIGVLGAGHLGKIHLRLLKEIPAYELVGFYDPDDKQAEQVAA
                     QFGLHRFHSVDDLIDAVDAVDIVTPTLSHFDCARKALKKTRHIFIEKPIAHTVREARQ
                     INDLVKEAGVKAQVGHVERFNPAFLALKDVAMKPMFIESHRLAFFNPRGTDVSVVLDL
                     MIHDIDIILHLVRSSVKKVHASGVAVISDQPDIANARIEFDNGCVANLTASRISMKVM
                     RKMRLFQRDAYISVDFAEKETEIFTLADTNDDDDALKIEVDTGGKFSQKSIRLDKPEI
                     LPVNAIQEELTLFARAIAEDTETAVPVSDGFHALEVAHQILEKIQKNHFAQ"
     gene            9315..10976
                     /gene="lnt"
                     /locus_tag="PK742_01950"
     CDS             9315..10976
                     /gene="lnt"
                     /locus_tag="PK742_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQU75380.1"
                     /translation="MTAGISVKQAYLLSIFTGLCWWLSWPPIDQAWLAFFAFIPLLWA
                     LESQLATGFSGGKVRYWLIVYTGLLVWNVLTTWWVGKASVAGGLFANFANPALQSIPF
                     MLFRSARRHLNRKAAWVMLVTSWMALEYLHLRWEFTWPWLTLGNVFAGAHTWVQWYEL
                     TGVFGGTLWVWTINILMLEWLLKLSGKRKGNAVVIPFFRMGWVQLAGIIMLVVIPVTA
                     SLIRYGQVQDEGSPVRVTVLQPNFDPYTEKFSLSESAMLDKMLSLSATGLTDSTDYLV
                     WPETSVPQPIWLNRAAFSGPIRRIRSLTDQYPDLTTVIGINAFMEYDSKEELTPTARE
                     LVNRQMHDTVWYDAFNSSIQIDTSGRIPWYHKSKLVPGVERLPYPGTLKFMEYFTLDL
                     GGISGSLGTQKDRSVFYNSDSTGVGTAICYESIFGEYVTRYVKNGAGLLFIITNDGWW
                     GNTAGYKQHCAYASLRAIENRRSIARSANTGISCFVNQRGDISQATAWAEDAVITGVV
                     YANSRQTFYTRNGDYLARTACWVMVLLWLFTFVRNRTARLRSISK"
     gene            11000..11776
                     /locus_tag="PK742_01955"
     CDS             11000..11776
                     /locus_tag="PK742_01955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQU75381.1"
                     /translation="MNALPVVKEAGMFVAENRRQIPWGEIEKKGDRDFVTSIDKKSER
                     LLVKGLSAILPEAGFFTEEKTVEQGDSSLRWVVDPIDGTTNFIHRTPVTAVSVALMDG
                     DDLLIGIVYEVTLDELFYSWKDAGTSFCNSEEIHVSDCSEFRKALVATGFPYQREGRL
                     PAILETAGYFLEHCQDIRRFGSAATDLCYVACGRMDIYYESYLQLWDVAAGILIVRNA
                     GGTVVDFSGSEHLTGQQVLATNGRFIEESLSGIRIPPITR"
     gene            complement(11766..12776)
                     /locus_tag="PK742_01960"
     CDS             complement(11766..12776)
                     /locus_tag="PK742_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQU75382.1"
                     /translation="MELIIIVTIIIALAYDFLNGMNDAANSIATIVATRVLSPGLAVI
                     WAAFFNFAAIWIFGQEVSSTMSKGVIDQGIVDGDNYFIFCALTGAMLWVWICTNYGLP
                     ISVSHALIGGLIGPAVMKGGADTLIWFTEDGKGLGLIFLFIFLAPIIGMFVAYVLQII
                     TLWLFRKSRPNRVDFLFRILQLSSSAAFSLGHGGNDAQKTAGVIILLLASAGLADIDQ
                     PPMWVFYMCYSVISLGTMLGGWKVIRTMGHNLTPLRPMGGFCAETAGAITLFATGSMG
                     IPASTTHTITGAIMGVGASRRASAVRWQVVYRIIGAWVFTIPATTMLSSLIYLLVSAI
                     NG"
     gene            complement(12776..13423)
                     /locus_tag="PK742_01965"
     CDS             complement(12776..13423)
                     /locus_tag="PK742_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQU75383.1"
                     /translation="MNIDRILRFFSPKDRVFFPLFLQAADNVVDMSVLLLKVVQEDDM
                     QKRQELIKDIERAEHKGDEITHEISNQLNTVFITPFDREDIHYLSTSIDDIADHVKDA
                     ALRIKLYQPAQMPAPLIKMTELITEGTRLVRSAISELSEIREVGRIRELTLSINNIEN
                     QSDTIFELALADLFSNEKDPIELIKIKDILSKIESASDRCEDIANVIDSIIVKTT"
     gene            complement(13484..15499)
                     /locus_tag="PK742_01970"
     CDS             complement(13484..15499)
                     /locus_tag="PK742_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU75384.1"
                     /translation="MKYNALLFFLFWSGSAWAQQPNIIFITIDDLNDYVEGFDGHPQT
                     ETPNISRIADHGTSFLRAYASAPQCGPSRLSLFTGKDCRYTNVYHNGDYKCGSFRANF
                     TAEEQNEIVYTLPEVLKNSGGYFTYSISKFFHCHNNLPDFDGDTPNTCDRSFSWNQYL
                     VFGSGGDEDIILEYGNEHDDGVGGFQFAPIPNGMEESMQDYLAVDSAIAFLDAYASNP
                     EQFCDRPFFLGLGFRRPHGPLFIPSKYYLEYYNNDLSQDPFNIPYNQPNEAYPYNGVV
                     MPPQPSLPWNDYFNLPSGGVAQALATDNGVHDHFQSFAFDLDLDGNLPVIDAGLSSLE
                     RYAIVNDAERANAVMAYLAAIRYTDAQIGRLYDALEEHPELLSNTIIVILGDHGYSLG
                     EKAHWRKGAMWETDTRAPLIISDLREDPVTGGTYTYRLVSFLDIFPTILELAGVEEPL
                     FEDGNRYLDGHSLVPLLEDEKATWYRPVISSYRNSPDTTQEGSCFVQYSVRTPDYHYI
                     RYQSNGADGFCNELNSNREEELYHLGEFNEIDPYEWNNLTGSEGAAAIIAILDDYLPD
                     SSMYNQLITYNVISSGITANDAPSIFLYPNPAKDACILHTEIPDEISHIIVSDMLGNT
                     RLKLSAYDLAGNASAPVLDISSLPAGQYMVTVYSQGGRPFTTRLIVL"
     gene            complement(15539..17332)
                     /gene="aspS"
                     /locus_tag="PK742_01975"
     CDS             complement(15539..17332)
                     /gene="aspS"
                     /locus_tag="PK742_01975"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQU75385.1"
                     /translation="MSSATYYRTHHCGELRLSDAGVRVTLAGWVHRSRDLGGMTFIDL
                     RDRHGIIQLAFNMETQALLCEKARKAGREDVIRVSGTVTERSSKNAHLATGEIEIVVT
                     DLEFLNHSETPPFTMEDQTDGGEELRMKYRYLDLRRPFMQQKFILRAALIKAVREYLD
                     ENGFLEIETPNLIKSTPEGARDFIVPSRMQPGNFYALPQSPQILKQLLMVAGMDRYYQ
                     IVKCFRDEDFRGDRQPEFSQIDCEMSFVRQEDIWQMFEGMVKHVFRKLQQTELPDFLR
                     LPYRDAIARFGTDKPDMRFGCEIKELNEVCTGSEFAVFNQVLTNNGLIAGLVATGCAG
                     YSRKQIDSLTDYVKAPHRGAGGLIYIRYEADGSVKSSVDKFFSPEHLKKILEQAGAVA
                     GDLLLIVADKTSKTRKVLGDLRLELARMNDWIDPAAWSIFWVVDMPMFEPDEETGEPI
                     FAHHPFCSPHPDDVEFFDTDPFRVRAQSYDLVMNGNEILSGSIRIHQPALQRKIFQIL
                     GFSEQEIESRFGFMVNAFRYGAPPHGGCAFGLDRWVMLMAGGETIRDVIAFPKNNAGK
                     DLMMEAPSPVDDAQLKMLHLDIIPTENKGSA"
     gene            complement(17386..>18961)
                     /locus_tag="PK742_01980"
     CDS             complement(17386..>18961)
                     /locus_tag="PK742_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75386.1"
                     /translation="LPESKRKFPGPSVAFFKAFGLSALFPKSRIFGQYHLGYLDEHAT
                     HEVDVLAGAFMLLRKEALVKTGLLDETFFMYGEDIDLSYRIRLAGYKNVYFHEAPIIH
                     FKGESTKKGSLNYVQMFYNAMKIFARKHFSGQNRGLFIFLINLAIYFRAGITLLAGFT
                     RRFTPVLTDLAIIFVSLFAVKEYWEYYVRYIDGGTYPDSYLYINIPVYASIWILSMYL
                     SGSYDRDSNPLRILRGIFWGTIVTAAVYGFLPEHLRFSRGMIVAGAATSAALLVGSRY
                     VWQLFRFGHFRFGESRSHRILLIGHEQEARRAFSQLESYGISQRLTGFCGEGSDQNGL
                     ARMGSMQELTVLLDQLKPGELIYCLRDTGYKDMISFMDANAGRYFFMMLPKAGPTILG
                     SHSKNNSGYQYDLRFNITTPYNRRLKRLSDILIACFVLLTFPVQLLLINHPAGAFRNA
                     IAVCSGRKTWVGYGPGKDPAFRIPSLQDGVLHPSLSEGTVNERMIALQNSLYAREYTL
                     ADDLRILIRNYRQLGR"
BASE COUNT         5047 a         4279 c         4594 g         5041 t
ORIGIN      
        1 atggtacgtg cggtacagga gggacatgct atgaaccgaa aaggcgtttt gttctggctg
       61 ggtctgacca taataggggg tctgatattc gtaggaagtc aggcttttga gtggtctcac
      121 ctgatccatg aaggaatgac ccagaaaagt aatcctttcg gagcatcagc tttcggtgga
      181 ttgttctttc tcatcaccgg attccatgga tttcatgtta gcgtgggcgt tatgttgaac
      241 tcctgggctt tcctgatgac attctttggt gcgtttcacc gtcgcggtca ttatgaaatg
      301 attgaaaagc ttggactgta ctggcacttt gttgatctgg tctgggtatt tgttttcctt
      361 gcattctatc tactgtaaaa gcggttataa tatgagcaat ctgacacacg aagaatctat
      421 caaaaaggtc tggcgggtat gtggtatcct ggcctttgtg accatctttg aaatagccgc
      481 cgccctgttt catttttata gtattccgga aagccccagg tggtggttga acctgctgtt
      541 catcatactc agtgctgcca aggcatacta tatcatgagt gagttcatgc acctgaagta
      601 tgagctcaag gccatgacgc tgacggtcat gactcctttc ctgttcttga tctggggaat
      661 tattgcattc ctctgggagg gaggctattg gctcagtctg cgggagttct ggcaatagac
      721 atgcaacagc agaggtcctg gttctggatg gtggtgatgg cgatcctcct gatcgttttg
      781 ccattgataa gctacctgct tgttagacag ggagcgttga tcaggcaaca tgccgctgcc
      841 agcgaatata taaaagagct cccgggaggc cttccccctg atcaactgtt catttctcaa
      901 cgtggtgata cccttacagc agagagacta ctgggaaaag ttgtggtggt ggaatgtatc
      961 aacagcacat ctgccggaaa gaccggagaa aggcatccgc ttttcgagat acaggaggca
     1021 tatgaaggca agaccctgaa cctgcggttc atgtctgtat gggaagatac cggtagagca
     1081 gccaccactg ctgatctggc ttatttcggt tccaggtttg ctgtaagaga gaattggcat
     1141 gtggtcagtt gcgggcagcc ggagtcctta ctgagttcta ttgacgaaaa cttttacaat
     1201 ggtgaaaaaa agtcaggaga ttctgacctt ttttttcctc atgttgttta tgtattgaac
     1261 aaggaagggc attggtgtgc tacctacaaa ctggatgacc agcaggctta tgatgccctg
     1321 tttaatgatt tgctatatct gatcgatcgt tgaagcgcaa tacctggata ctgatcatag
     1381 tggtaggtgt ggcttttgca gccttcttta aatgggtcta tctacccatg gcggccaggc
     1441 aggatcagat cagcaggatg gcagatgatc tgtatccaac agataaacta cgtcagcctt
     1501 cagctactat ccctcctttt tccatgaccg atcagaatgg gcaaacgatc acagagcagg
     1561 atgtaaaagg gcatattcat attgccgact ttttcttcac ctcctgcgat gccatttgcc
     1621 cgatgatgac ctctcagcta tcaagagttc aggaggccat gggcgatgag cgggattacc
     1681 ggatcttatc gttttcactc gattctgaaa atgactctgt tcccgtgctt cggtcctttg
     1741 ccagaaaatt tggtgccaac gatacggttt ggcatctgct taccggcgat aagaaagcca
     1801 tttatacatt gggtgcagaa ggctacaaac agtcggtatt gagagatgca gaaaatgaca
     1861 tcaatcatag cggaagattg atcctggttg accggaacct gcagataaga ggattctaca
     1921 atgcacttga ttctttggaa gttgatatgc tgatcaatga cctgtcttac cttttgtaca
     1981 aagaagaatg gaatgagtga gcgcctgcaa aacagattga tctggatcat cagtattgcc
     2041 gtgccggcag tggtggctat gctgttcttc atgcctggcc tggacctggg cacagatgtt
     2101 tccatgttgc ccggacttaa tgcagttatc aatggaacag tatctgtgtt gctcatagcc
     2161 ggttatatcc tgatccgctc agggaaaaga caagcccata gagccgctat gcttagtgct
     2221 ttttttctgt ctgccttatt tctgatatca tatgtgatct atcattcggc caccgaatcg
     2281 acttcgtttg gtggtgccgg gttgatcaga acagtttatt tcttcatact cattacccat
     2341 atcgtactgg ctgcagccat cctgcccctg atcctgatca ccatgtccag agcattgtct
     2401 tccaggtttg acaggcacag aaagatcgcc cgaatcacat ttccgctatg gttgtatgtg
     2461 tctgttacgg gtgtagtcgt atatttgatg atcgctcctt attattgata tgaaagttag
     2521 aatgctcttt tttctggtaa tgttgtttct gttgatcggg gtttcatcgg cagacgcaca
     2581 gtgtgctatg tgtaaaatgg cggctgaaaa tggcgctcag gctgatggaa agccatacct
     2641 ggcactcaac cagggaatcc tctatctggc agccgtaccc tatctgtcat ttagtgtgat
     2701 cgcattttta ttctggcggg gttatcgccg gcgcagagag caggaagcag agctggaagc
     2761 ctgatcagat gtcacccaat tgagggcgta gtatgatctc ttctaccgta gcagtctcag
     2821 aaagcgcaat gcattgccag accacatttg caatatcctc aggctgcatg aatctggctg
     2881 caggcaatcc gctcgatgcc caggagtctg tgtaggtggc accaggcaaa acacttgtca
     2941 ctttgattcc atgtggcttc agctcttctc ttagattttt tgagaaacca agcagagcaa
     3001 atttggaaac tgaataggca cctccgtcag gataggcctg gatacttgct atggaacaaa
     3061 tattgataat atgaccggac ttacctgaca tcattccggg tatcaattcc cttgtcagat
     3121 gataggcact gtaaaaattt gtgttcatca ttgtttccat cgttccgggc ggttcattgt
     3181 ataaactacc cggtatgaat tgtccggcat tgttgataag aatgtctggc acaccaaatg
     3241 tttccagcac aattttacct gatgcctgag cctgagatat atcagaaaga tctgctgtat
     3301 gatagctgca ggaaacagta ttgccggctg agagtatgct gctgcaagtt cttttcaggt
     3361 ggtcttcaga tctggctatc aggaaaacat cacaaccctc tgatgccagc ttctcagcga
     3421 tggctgctcc aattccttta gatgctcctg taatgactgc ccgcatgaaa tttgtttaga
     3481 atactaaagt aaatacattt attttatgca ccgttgaata cactctatgc cgatattatt
     3541 ctgccttttg cagttccggg aacctttacc tatgaggtgc cggaagctgg ccgtgaactg
     3601 gcaattgcag gaatgcgttg cgaagttcat tttcgcaaga aaatacaggc cggaataatt
     3661 ttgcgaatac atcaagaaaa accatcaggt ttccaaccca gacagatcat tcagtttccg
     3721 gatgatgaac ctgtggtcag tacgttgcag ctgagactgt ggatctggat gtcaacttat
     3781 tatatgtgca ccctgggtga gatctaccag gcggcagtac cggctttgtt caaatcaggc
     3841 agtgaaactc agatctcttt gaatcagatg cttgatacgg atacacttga ccttgatgac
     3901 aggtctttta tcctggtgga agcactgaag caccagaaag taatgcaact taaagatgtt
     3961 caggaattat tgggcatcaa acagattcag tctctgctca ggaaactcgt catgaacaat
     4021 gtgatcctgt tgcaggaaga ggttagagat gtctataaac cgaaagtaag aaaactgatc
     4081 aggctaacag aaagatttca ggatgaagag gctttgaggc agctgtttga cgaattgaac
     4141 agggcagtca gacaacagca tgtactgatg atctatttgc agcttgcacc agaaggggaa
     4201 gcggtaagca gagcagtttt gttaaagaag gctgaagcat ctgctgcagt tctgaaggca
     4261 atgacagaca agggaatatt tgaagaaacc gaagaagtag taagcaggct aaaactttcc
     4321 ggtaatagca gcctcaccag gtcgaagtta agtttgacgc aacaagaagc tctgcattcg
     4381 atcagatcac agtggcagga aaaagaagtg gtactgttgc atggtgtgac cggcagcggg
     4441 aagactgaga tctacatgga acttatagag gaggttttgg agcggggtaa acaggtgttg
     4501 tacctcctgc ctgaaattgc ccttacagcg cagatcattg gcagattaca ggagagattt
     4561 ggagatcagg taggcatcta tcactcacgt ttcaatcatc aggagcgtgt ggagatctgg
     4621 cagaaagtgc agcaaggcag ttacaggata ttgctgggtg cccgttcagc tttatttctt
     4681 tcctttcagg atctgggtct ggtggtcatt gatgaagagc atgattactc cttcaaacaa
     4741 caggatccgg cacccagata tcatgcaaga gataccgcaa tttatttggc atcaatggtt
     4801 gcggctaagg ttctgctggg ctcggcaaca ccctctgtgg aagcttatca gcatgcacat
     4861 acaggaaaat ttggttatgt ggaactgaca gaaagatttg caggaatgga attgccggaa
     4921 atagagctga tcaatatgca atctgaaacg cagcagaata atgtagtgac ccactttagc
     4981 aggaagttgc tggcagcgat ggaagaagca gtggccggaa aggaacaggt catactgttt
     5041 cagaaccgaa ggggatatgc cccgtatgtt gtctgtactt cgtgtggatg gacgcctgtg
     5101 tgtgtgagat gtgatgtgaa gctggtgtat cataagtttt ccggagaatt gaggtgtcat
     5161 tattgtggtt ataaacagga gatcgtaaca gcatgtcctg aatgtgcttc tacacagatc
     5221 atcgttcagg gttttggaac agaaaaggtg gaagatgaac ttcaggcgct atatcctgcc
     5281 tggcgtatcg cacggctgga cctggatgca gtgcgcacca aagctggaca tgaaaaggtc
     5341 atacatgcct tcgaagacgg acaggtggat gtgctgatag gcacgcagat ggtaacaaaa
     5401 ggtcttgatt ttgataatgt caggctggtt ggaatactca gtgctgatca gttgttgcag
     5461 tttgcagatt tccgggcatc tgaacgggct tatcagctca tgatgcaagt aagcggaagg
     5521 gcaggcagga agcaaaagcg gggaaaagtt ctgatacaga cattgcgctt ggatcatccg
     5581 gtgctggggt tcgttcagcg gcatgagacg catacctttt atgaagaaga aatcagacaa
     5641 agaaggaatt ttaagtaccc gccattcaac cgtattattc gtattacgct gcggcatgct
     5701 gaaccaggtt tgtcattgaa cgcagcatat ggccttgtga atgcattggg aaatggacct
     5761 gcttttgagg tattaggccc cgttcagccg gctatagcaa ggataagagg taagtacctc
     5821 tcggaggtca tgatcaaggt caggaatgac ggtcagtctg caaaagcagc caagcaaaga
     5881 ctgcggcagg caatggaaag agttgcagca aaaaaggagt ggaagaaagt agagatcata
     5941 ccggatatag atcctgtctg atcattttcc ttttccctga aaaataatga ccagggtgta
     6001 gaacatgatc ttaaaatcca tcgcaagact catgttttct atatagagca ggtcgtattt
     6061 caatcgctcc accatctcat ccacattgct tgcatatcca aatttcacca tacctaaaga
     6121 ggtcagtccg ggcttcacct tttgcagatg tttatattca ggggcccttt gtacgatctg
     6181 atcgatatag tatttccttt caggacgggg ccccaccagg ctcatatcgc ctaagagaat
     6241 gttatagaac tgagggagct cgtcaaatcg ccattttcgc atgatctttc cccaggaggt
     6301 gatgcgctgg tcttcttcac tagacaatgc cggacctgtc ttttcagcat cctgaaacat
     6361 agacctgaat ttgtagatag taaatggttt gccattgatt ccaattcgcg tctgtttata
     6421 catgacaggt cctgaagaag acagtcgaac cttgatggca atgaacagat ataatggcca
     6481 gaggatgcag aatacgaaaa tactgatcac aacatccagc attcgtttga tggttctttg
     6541 ccatttgggc atcaggtcag ggtagatctc aatgagcacg gcatcaaata cattgctcat
     6601 tttaactgaa cctgacatga tatcatacat gtctgggatg atcttaacca ctacaccacg
     6661 caggtcagat aaacggttca taatgtcatt gagcaaatgg tgctcagatg tttcaatggc
     6721 aatgataact tcatctatgg accggtgcag aagtatgtcg tccagttggt caagtccgcc
     6781 caggtgggga atgtacttgc tgagttcatt gccgttctgc ccgttggcat caatgtaacc
     6841 tataaaggag tagccaagtg atttcttttt cctggtaact tccatataca gatcaagcgc
     6901 tcttctgttg ccaccaatca acaaagtatt gtaagcaatc tgcccgcttt gaagttggcg
     6961 cttggcaagc gtcagaatgg tcattctgga tccggtagtc agaaggaact gactgcctgc
     7021 aaggatcaga aaggtctggt aataatcctg gtagccttca atgtaatcat ccagtatcag
     7081 gacaaagaac agtaccatgg agccaagaaa tgcagccaga aaagttctgt agaattctgc
     7141 aagtctggat tttctgtaga tatctgtata gaagccggca acgaagtaga tgaggatcca
     7201 gccacaggct actgcagccg ctcccagata aaacttcggg tcactgaata catactcctg
     7261 cagatcaaat ggacgatctt ctatgaagaa tttcctgaat gcaaaggtga cacaccaggc
     7321 aagagctgca gcaacaagat caaagaacag ataggttatc agaccaattt tcttccttcg
     7381 ccgcataatc agaaagcaat gatcttgatg ttgtcaaaat acagttcagc tgaactgaca
     7441 gaatcaggct tataacaacg gaattccaga ttgtatgttt cggcctgaag ggtagccact
     7501 ttagcggcca gattgaggta gatcttattc cagtcattac gtgcacctac ggtcagcatg
     7561 taggaactga tagggattcc gccagtgtag tttccattca cccaaacttc aaatggtaca
     7621 cttgccttat agtcaacctc tgcccaaacg gcattgccaa ctgtaggaaa aagcaatggc
     7681 ccggtagtca ctttcatgac actgatgccg ggctccatgt tggcaaaagc agagcggctg
     7741 ccttctaata ccaattccgg atcggtcacg atctccaggc cggtttcact tcccgatagc
     7801 tgggtaaatg agttgcccag ttcaaagcgc tccaggtaaa tccatgaaag tcccgacaga
     7861 tagtcaactg aaggttggat cgaatccgtc tcaaatggtc tcagggtaag tgttgtttca
     7921 aagcctgagt aaaaaggata gatattacca atactgctga aaccgttttc cttgatacct
     7981 gcaaaaacac tgatgcttac agttcctgta tcgagaacag gaactgtaac aggtaattca
     8041 aaaacaccca gttgaatacc attcacataa acccatgcat cggtgatctt ctgtgtagga
     8101 gacccttcag accccgggtc acttatcacc tcaaaatctg ctatatgtac gaatgcaggt
     8161 tgcagctcat cgccggtgca tgcggaaacc aacacaggta tcatgagaag atattgcagg
     8221 tgccgtttca ttgggcaaaa tgatttttct ggatcttctc cagtatctga tgtgccacct
     8281 cgagggcatg gaatccatct gatacaggaa cagctgtttc tgtatcctcc gcaatggcac
     8341 gtgcgaataa ggtcaattct tcctgaatgg cattcacagg caggatctca ggtttatcca
     8401 ggcggatgga cttctgcgag aacttcccgc cggtatccac ctcgatcttt aacgcatcat
     8461 catcatcatt tgtatctgcc aaagtgaata tttctgtctc tttttcagcg aagtcaacgc
     8521 tgatatatgc atcccgctga aacagccgca tcttccgcat aactttcata ctgatgcgac
     8581 tggcagtcag gttggcaacg caaccgttgt cgaattcgat ccgggcgttt gcaatatcag
     8641 gctgatcact gataacggcc actcctgatg catgtacttt ctttacggaa gacctcacca
     8701 gatgcaggat gatatctatg tcgtggatca tcaggtccag aaccacgctt acatctgttc
     8761 ctcttgggtt gaaaaatgcc agccggtgag attctatgaa catgggtttc atggcaacat
     8821 ccttcagggc cagaaacgcc ggattgaatc gctctacatg tccaacctga gccttgaccc
     8881 cggcttcctt taccaggtca ttgatctgcc gggcttctct tacggtgtgt gctataggct
     8941 tttcaatgaa aatatgccgg gtctttttca gagccttccg ggcacagtca aaatgagaca
     9001 gggtaggtgt aacaatgtca acggcatcta ctgcatcgat cagatcatcc acactgtgaa
     9061 atctgtgtaa tccgaactga gcggctacct gttcggcctg cttatcatcc gggtcgtaga
     9121 aacctaccag ctcgtaggca gggatctctt tcagtaacct gagatggatc ttccccaaat
     9181 gtcctgcacc caatacgccg atcctgacca tgatattggt gcaaatgtac acagctaaag
     9241 tcaggataca taagacttac aggccattat actgccacca tcgggctccc gttatttatc
     9301 ctatcttcgg ggcgatgact gcaggcattt cagtaaagca ggcttattta ctttccattt
     9361 ttacaggact gtgctggtgg ctgagctggc ccccgatcga tcaggcctgg ctggcgttct
     9421 ttgcatttat acccttgtta tgggctttgg aatcgcagtt ggcaaccggg ttcagcggtg
     9481 gaaaggtcag atactggctc atcgtttaca ccggtctgct ggtctggaat gtgcttacta
     9541 cctggtgggt gggaaaagca agtgtggcag gtggattatt tgccaatttc gccaaccctg
     9601 cactgcaatc tattcctttt atgcttttca gaagtgcaag gcgacacctg aacagaaaag
     9661 ctgcttgggt catgctggtc acatcctgga tggcgctgga gtacctgcat ctgagatggg
     9721 agttcacctg gccatggctg actcttggaa atgtatttgc tggggcgcat acctgggtgc
     9781 aatggtatga actgacaggt gtctttgggg gaactctttg ggtatggacc atcaatatcc
     9841 tgatgttgga atggttgctg aaactcagcg ggaaaagaaa aggcaatgct gtggtaatcc
     9901 cttttttccg gatgggttgg gtacagctgg caggcatcat catgttggtt gttattccgg
     9961 tcaccgcatc actgatcaga tatggtcagg tgcaggatga gggaagccct gtccgtgtaa
    10021 ctgtactaca gcccaatttt gatccttata cagaaaaatt cagcctgagt gaaagcgcaa
    10081 tgctggataa gatgctgagc ttgtctgcaa ctggattgac agactcaaca gactacctgg
    10141 tctggccgga aacctcggtt cctcagccca tctggctgaa ccgggcagca ttcagcgggc
    10201 caattcggcg gatcagaagt cttactgatc agtatccgga tctgacaacc gttattggca
    10261 tcaatgcatt tatggagtat gattccaaag aagaactgac tcctactgcc cgtgaacttg
    10321 tcaacaggca aatgcatgat acagtatggt atgacgcatt taattcatct attcagatag
    10381 atacttcagg tcggattcca tggtaccata agtccaaact tgtgccgggt gtagaacgat
    10441 tgccatatcc gggaacattg aagttcatgg aatactttac gcttgatctg ggaggtatat
    10501 caggatcact tggtacacaa aaagacagat cggtatttta taacagtgat tccacaggtg
    10561 tgggaacagc catctgttac gaaagtattt ttggtgaata tgttacccga tatgtgaaaa
    10621 atggcgccgg tctgttattc atcattacga acgacggctg gtggggaaat acggcaggtt
    10681 acaaacagca ctgtgcatat gccagccttc gtgctattga gaacagaaga tccatagccc
    10741 gctcagccaa tacaggaatt tcctgttttg tgaaccagcg gggtgatatc agtcaggcaa
    10801 cggcatgggc cgaagatgcc gtgatcacag gggtcgttta cgccaacagt aggcagacct
    10861 tttataccag gaacggcgat tacctggccc gcactgcctg ttgggtgatg gtgctcttat
    10921 ggctattcac ctttgtcaga aatcgtacgg cacgattaag atctatatca aagtaattat
    10981 gcatcttgag tctatatgta tgaatgcact gccggttgtg aaagaggccg ggatgttcgt
    11041 tgcagaaaac cgcaggcaaa ttccctgggg agagatcgag aaaaaaggag accgcgattt
    11101 cgttaccagt attgataaaa aatcagaacg actcctggtg aaagggcttt ctgcaatact
    11161 cccggaagca ggattcttta cagaagaaaa gacagttgaa cagggagata gcagcttgcg
    11221 ctgggtggta gatccgatag atggtaccac caatttcatt catcgcacac ctgtaactgc
    11281 tgtaagtgta gccctcatgg atggcgatga tcttctcata ggtattgtat atgaagtgac
    11341 ccttgatgaa ctgttctatt cctggaaaga tgcaggcact tcgttttgta acagtgagga
    11401 aattcatgta agcgattgca gtgaattcag aaaagcattg gtggctaccg gatttcctta
    11461 tcaacgggaa ggaagactgc ctgccattct ggaaactgcc ggatatttcc tggaacattg
    11521 tcaggatatc agaaggtttg gttcggcagc tacagacctg tgctatgtag cctgcggcag
    11581 aatggacatt tactacgaat catatctgca gctgtgggat gtagctgcag gtattctgat
    11641 agttcgcaat gccggtggta cagtagttga tttttcagga agtgaacatc tcacggggca
    11701 gcaagtgctg gccaccaatg gcagattcat agaggaatca ttatcaggga tcaggatccc
    11761 tccaatcacc cgttaatggc tgaaacaagc aggtagatga ggctgctcag catggtcgtg
    11821 gccgggatgg taaataccca ggctccaatg atcctgtaca ccacctgcca acgcacggca
    11881 gaagctcttc tgctggcacc aacccccata atggctccgg taatggtatg tgtggtactg
    11941 gcaggaatac ccatggatcc ggtggcaaat aaggtgatag ccccggctgt ttcagcacag
    12001 aatccaccca tgggtctgag gggggtcagg ttgtgcccca tggtacgaat gaccttccag
    12061 ccaccaagca tggtgcccag agatatgacc gagtaacaca tgtaaaacac ccacatggga
    12121 ggctggtcaa tgtctgccag gccggcactg gccagtagca gtatgataac cccggcagtt
    12181 ttttgtgcat cgttgccgcc gtgtccaaga ctgaatgcag cagaagaaga caactgcaga
    12241 atgcgaaata aaaagtccac cctgtttggt ctggattttc tgaataacca aagcgtgatg
    12301 atctgcaaca cataggcaac gaacatacct attatcggag ccagaaaaat aaacaggaat
    12361 ataagaccaa gaccctttcc gtcttctgta aaccagatca gggtatcggc acctcctttc
    12421 atgactgccg gcccgattag tccacctatc aatgcatgcg aaacactgat tggcaaaccg
    12481 taattggtac agatccagac ccacaacatg gcacctgtca gggcacagaa tatgaaatag
    12541 ttatcgccgt ccacgatccc ctgatcaatg acccctttgc tcatggtact gcttacctcc
    12601 tgtccgaata tccagatggc agcaaagttg aaaaaggcag cccagatcac agcaagaccc
    12661 ggactgagca cccgggtggc tacaatagta gcgatgctgt tggcagcatc attcattcca
    12721 ttcaggaaat catatgccag agcaatgatg atggttacaa tgatgatcag ttccattagg
    12781 tggttttcac tatgatggaa tcgatcacat tggcaatgtc ttcacaacgg tcagatgcac
    12841 tttcaatctt actcagaata tccttgatct tgatcagttc gataggatct ttttcattgg
    12901 aaaaaagatc tgccagggct aattcaaata tggtgtctga ctggttctct atgttgttga
    12961 tgctgagcgt gagttctctg atacgaccaa cctcccgaat ttcactcagt tcactgatag
    13021 ctgaacgaac aagtctggtg ccctctgtga tcagttctgt catcttgata aggggtgcag
    13081 gcatttgtgc cggctgatac aacttgatgc gaagtgctgc atcttttaca tgatctgcaa
    13141 tatcatcaat ggaggtggac aggtagtgaa tatcctccct gtcaaaaggt gtaatgaaaa
    13201 cggtattcag ttgattggaa atttcatgtg tgatctcatc tcctttgtgt tctgcccgtt
    13261 ctatgtcttt gatgagttcc tgcctttttt gcatatcatc ttcctgtact actttgagta
    13321 gtaatacaga catgtccacc acattgtcag cagcctgcag aaacagtgga aaaaagaccc
    13381 tgtctttggg cgaaaagaaa cgcagtatcc tgtctatatt catggatggg gtattatact
    13441 cttaaaggta cggggttcag acggaataac cagccgggag aaattatagt acgatcaacc
    13501 gggtggtgaa aggcctgcca ccctgactat aaacagttac catatactga ccggcaggca
    13561 acgaacttat gtcaagaaca ggagctgagg cgttacctgc cagatcatat gcggatagct
    13621 tcaaccgggt attgcccagc atatctgaga ctatgatatg actgatctca tccggaattt
    13681 ccgtatgcag tatgcaggca tcttttgcag gatttgggta gaggaagatg gaaggagcat
    13741 catttgctgt gatccctgat gatatgacat tgtatgtgat caactgatta tacatactgc
    13801 tgtctggcag gtaatcatcc agtatggcaa taatggctgc agcaccttca ctacctgtca
    13861 ggttgttcca ctcatacgga tcgatctcat tgaattcacc cagatggtat agttcttcct
    13921 ccctgttact gttcagttca ttgcaaaaac catcagctcc attgctttgg tatctgatat
    13981 aatggtaatc aggtgttctg acagaatatt gcacaaagca ggaaccttcc tgtgtggtat
    14041 cagggctgtt ccggtatgat gaaatgacag gtcggtacca ggtggctttt tcatcttcca
    14101 gcaaaggcac gaggctgtgt ccatccaggt agcggttgcc atcctcaaat aatggctctt
    14161 caactcccgc cagttcgagt atggttggaa aaatatccag gaaactcacc aatctgtagg
    14221 tataggtccc gcctgtgacc ggatcttcac gaagatcaga aatgatcaaa ggtgccctgg
    14281 tgtctgtttc ccacatggca cctttgcgcc agtgtgcttt ttcgccaaga gaataaccat
    14341 gatcgccaag aataacaata atggtgttgg agagcagttc gggatgttct tccagtgcat
    14401 catataacct tcctatctga gcatctgtat aacgtatggc agcaaggtaa gccatgactg
    14461 catttgcccg ttctgcatca tttacaatag catatctttc gagcgaactt agtcctgcat
    14521 ctatgaccgg caagttaccg tccagatcca gatcaaaggc aaaggactgg aaatgatcat
    14581 ggactccgtt gtcagtagcc agggcctgag ctactccacc tgacggcagg ttgaaataat
    14641 cattccaggg taatgatggt tggggcggca tgaccacacc attgtacggg taggcttcgt
    14701 taggttggtt gtacgggatg ttgaacggat cctgactcag gtcattgttg taatactcca
    14761 gataatactt tgacggaatg aacaaggggc cgtgcggcct tctgaatcca agacccagaa
    14821 aaaatggccg atcgcagaac tgttcaggat tgctggcgta tgcatccaga aaggcgatgg
    14881 cagaatcaac agccagatag tcctgcatac tttcttccat accgtttggg atcggggcaa
    14941 attgaaaacc acctacaccg tcgtcgtgct catttccata ctccagaata atgtcttcat
    15001 caccacctga gccaaatacc agatattgat tccaggagaa agaccggtcg catgtattcg
    15061 gggtgtctcc atcaaaatct ggcaggttat tgtggcaatg gaaaaacttg ctgatggaat
    15121 aagtaaaata tcctccacta ttcttcagta cttcgggtaa ggtatagact atttcatttt
    15181 gttcttctgc agtgaaattg gctctgaatg aaccgcattt gtagtcgccg ttatgatata
    15241 cattggtata tctgcagtct tttcctgtga acagactcag tctgctcggt ccacactgcg
    15301 gagcactggc atatgctctc aggaaagaag ttccatgatc tgcaattctg gaaatgttgg
    15361 gtgtttcggt ttgtggatgg ccatcaaaac cttccacata atcattgagg tcatcaatgg
    15421 ttatgaaaat tatattgggt tgttgtgccc aagctgatcc tgaccagaac aggaagaaca
    15481 aaagggcatt gtacttcatc tcagcaaaaa gctaaggtaa caaacatgcg ggccgggatc
    15541 aagcagaacc tttattttca gttggaataa tgtccaggtg aagcattttt aactgtgcat
    15601 catcaaccgg cgacggggcc tccatcatca gatcttttcc ggcattgttc tttggaaaag
    15661 caatcacatc gcgaatggtt tctccgccgg ccataagcat cacccagcgg tccaggccaa
    15721 atgcacagcc accgtgcgga ggggctccgt acctgaacgc attgaccata aagccgaacc
    15781 gactttctat ctcctgctca ctgaacccaa gaatctggaa gatcttacgc tgcaaggctg
    15841 gttgatgaat tcggatgctt ccactcagga tctcattacc attcataacc aggtcgtaag
    15901 attgtgcccg cacacgaaaa ggatcagtgt caaagaattc aacatcatcc gggtgcggac
    15961 tgcagaacgg gtggtgtgca aagatgggct cacctgtttc ttcatccggt tcgaacatag
    16021 gcatatcaac cacccagaaa atactccagg cggcaggatc gatccagtca ttcatacggg
    16081 ccagttcaag gcgcaggtcg cccagaactt tacgggtctt gctggtcttg tctgccacga
    16141 tcagtaacag gtcgcctgca accgctccgg cttgttccag gatctttttt aggtgttcag
    16201 gagagaaaaa tttgtcaaca gaggatttga cggaaccgtc tgcttcatag cgaatgtaga
    16261 tcaaacctcc tgcaccacgg tgcggagctt ttacataatc agtcaggctg tctatttgtt
    16321 ttctggaata tcctgcacaa cctgttgcaa caagacctgc aatcagtcca ttgttggtta
    16381 gtacctgatt gaatacagca aattcactgc cggtacaaac ttcattgagt tctttgatct
    16441 cgcagccaaa tcgcatatcg ggcttgtctg tgccaaaacg tgcaatggca tcacggtaag
    16501 gtaaacggag aaaatcaggc agctctgtct gctggagctt gcgaaagaca tgcttaacca
    16561 ttccttcaaa catctgccag atatcttcct gacgcacaaa actcatttcg cagtctattt
    16621 gtgagaattc cggctgacga tcacccctga aatcttcatc gcgaaagcat ttcacgatct
    16681 gataatatct gtccatcccc gcaaccatga gtagttgttt aaggatctgc ggagactgtg
    16741 gcaacgcata gaagttgccc ggttgcatcc tggaaggaac aatgaaatct ctggctcctt
    16801 caggagttga cttgatcagg ttgggagttt ctatctccag aaatccgttt tcatccaggt
    16861 attctctgac ggctttgatc aaagcagcac gcaaaatgaa tttctgctgc atgaaaggtc
    16921 gccgaagatc gagataccgg tatttcatac gcagttcttc tccaccgtct gtctgatctt
    16981 ccatggtaaa cggaggtgtt tcactgtggt tcaggaactc cagatcagta accacgatct
    17041 ctatctcacc agttgccaga tgtgcattct tactgctccg ttctgtaact gtaccactta
    17101 cacgtatcac gtcttcccgt cctgcctttc gtgctttttc gcacaacagt gcctgtgttt
    17161 ccatattgaa cgcaagctgg atgatgccat gtctgtcgcg aagatcaatg aatgtcatac
    17221 cccccagatc acgactccgg tgaacccatc cggccagggt aacccggaca cccgcgtctg
    17281 acaatctcag ttcaccacaa tggtgggttc tgtaataagt tgcactgctc atttgcttca
    17341 gaaattaaag attcaaaggt acacagacaa ggtgataatg acgctctaac gccctaattg
    17401 acggtagttt ctgatgagaa tacgcagatc gtcggccagg gtgtactcac gggcatacag
    17461 gctgttttga agggcgatca tacgttcgtt gacagtgcct tctgacagag agggatgcaa
    17521 tacaccgtcc tgcagggaag gtatgcgaaa tgcggggtcc tttcccggac catagcctac
    17581 ccaggtcttt ctgccggagc aaacagctat tgcattgcga aaagcacccg caggatggtt
    17641 gataagcagc agctgtacag ggaaggtcaa tagtacaaaa caagcaatga gtatgtctga
    17701 aagtcttttt aaccgcctat tgtagggagt ggtaatattg aaccgcagat catattgata
    17761 tccgctgtta ttcttggagt ggcttcccaa aatagtgggg cctgcttttg gaagcatcat
    17821 aaagaagtac ctgccggcat ttgcatccat gaatgatatc atgtctttat atccggtatc
    17881 acgaagacaa tagatcagct cgcccggctt gagctgatcc agtaatacag ttaactcctg
    17941 catgctaccc attctggcca gaccgttctg atcacttcct tcgccacaaa accctgttag
    18001 tcgctggctg attccatagc tttccagctg actgaaagca cgcctggcct cctgttcatg
    18061 tcctattaac agaatgcggt gtgaccgcga ttcgccaaac ctgaaatgac cgaaacgaaa
    18121 cagctgccaa acatatctgc tacctaccag caacgcagcg ctggtggcag cgcctgctac
    18181 gatcatgcct ctgctgaacc tcaggtgctc gggcaggaag ccataaactg ctgcggtaac
    18241 gatggtgccc cagaatatgc cacgcaggat gcgcaatgga ttcgaatcac ggtcataact
    18301 gccactgagg tacatggaca ggatccaaat ggaagcatat acaggaatat taatatataa
    18361 ataactgtct ggatacgttc ctccatctat gtagcgaaca taatattccc agtattcctt
    18421 gacagcaaac aacgagacaa aaatgatggc caggtctgtc agaacaggag tgaacctcct
    18481 cgtgaacccg gccagcaggg taattccggc ccggaaatag atggccagat tgatcaggaa
    18541 aataaacagt cctctgttct gccctgaaaa atgtttgcgt gcaaagatct tcatggcgtt
    18601 gtagaacatt tgcacataat tcagactgcc tttcttggtg ctttctccct taaagtgaat
    18661 gatgggcgct tcgtggaaat acacattttt ataacctgcc agcctgatgc ggtaagacag
    18721 gtcaatatct tcgccgtaca taaaaaaggt ttcatccaga agcccggtct tgaccagagc
    18781 ttcttttctg agaagcatga acgctcccgc aagtacgtcc acttcgtggg tagcatgttc
    18841 atccagataa cccaggtgat attgaccaaa tatcctgctt tttggaaaaa gggcagataa
    18901 gccaaacgcc ttgaagaagg caactgaagg acccgggaac ttccgcttgg attcaggaag
    18961 a
//
LOCUS       DAOMAX010000016        18877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_300_length_18877_cov_4.537456, whole genome shotgun sequence.
ACCESSION   DAOMAX010000016 DAOMAX010000000
VERSION     DAOMAX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18877
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_18877_cov_4.537456"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..371)
                     /locus_tag="PK742_01985"
     CDS             complement(129..371)
                     /locus_tag="PK742_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQU75387.1"
                     /translation="MSFLYFILIGAAAGWISGKLIRGEGFGWLGNIIVGIVGAVLGGW
                     IFGQMNIASSGLAGSLASSVVGAVVFLFLIGLITRK"
     gene            complement(368..2194)
                     /locus_tag="PK742_01990"
     CDS             complement(368..2194)
                     /locus_tag="PK742_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQU75388.1"
                     /translation="MSSPRSRRKAPVEERDKKRLNASSLRTAMRIFSFLRPYRGMFIA
                     GMSFLLISSFTTMTFPFFMGKLIDTAGVEVEGDWLTNTNTVALVLVGILFVQAGISFF
                     RVYLFAVVTEKSMADVRQLLYDKLITLSIPFFEERRVGELTSRSTNDIDQLQDMLSMT
                     LPEFIRQIVTIVFGVGFIFFISAKLTLLMLSTFPVLIILAMLYGKFIRRLSKERQDAL
                     AESNIVLEETLQNVSTVKAFANEWFESIRYKGFLGRVVHAGIRGAVNRGAFTTFLIFG
                     LFGGFVLVLWYGAVLMKEGEVTAGTLTAFMLYTGFIGGAVGGIGDLYGRLQKTVGASE
                     RVMEIIDTDSEVDLQDPKEETPVRGEVTFSNVHFSYPTRTDVEVLKGIDMQIRSGERI
                     ALAGHSGAGKSTIAQLVLGFYQPSSGSISVDGRPYADYDLKHLRNNMALVPQEVILFG
                     GSIRENISYGKPGATDSEIEAAARKAHAWEFISGFPEKLDTVVGERGVKLSGGQRQRI
                     AIARAILKDPAILILDEATSSLDAESERLVQDALDTLMKDRTTIIIAHRLSTIRHADH
                     IFVLEDGRIAEKGNFEELANIDGGIFSGLLKLQFFDQEKPNI"
     gene            2253..2660
                     /locus_tag="PK742_01995"
     CDS             2253..2660
                     /locus_tag="PK742_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQU75389.1"
                     /translation="MLTHFDIILPAYPRGFHLIDEQVFGQIELPETGLLHIFIHHTSA
                     GLCINENADPTVRLDFETIFNKLVPEGLPEYLHNLEGDDDMPAHVKSVLSGASVSLPV
                     RNGRPVLGTWQGIYLCEFRNNGGRRKLTVSVVE"
     gene            complement(2709..3053)
                     /gene="rplT"
                     /locus_tag="PK742_02000"
     CDS             complement(2709..3053)
                     /gene="rplT"
                     /locus_tag="PK742_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQU75390.1"
                     /translation="MPRSVNHAASKARRKKVLKQAKGFWGRRKNVWTVAKNAVEKAHQ
                     YAYVGRKDKKAHFRQIWIARINAAVRPYGMTYSTFIHKVQEAGIELNRKALADLAMNH
                     PAAFEEIVNKVK"
     gene            complement(3064..3261)
                     /gene="rpmI"
                     /locus_tag="PK742_02005"
     CDS             complement(3064..3261)
                     /gene="rpmI"
                     /locus_tag="PK742_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQU75391.1"
                     /translation="MPKMKPNRSSKKRFKLTGSGKLKRSKAYHSHILTKKSNKRKKRL
                     QGTTVVHEADTPRIKRTLGLA"
     gene            complement(3338..3895)
                     /gene="infC"
                     /locus_tag="PK742_02010"
     CDS             complement(3338..3895)
                     /gene="infC"
                     /locus_tag="PK742_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQU75392.1"
                     /translation="MQRRGPRPPFRGRVKQNPNRINREIRVPKVRLVGEGIEAGVYST
                     EEAYRIAQEMELDLVEISPNADPPVCRIVDYQKFLYEKKKKEKELKAKNKKQEMKEIR
                     FGPNTDEHDFEFKTKHAEKFLQEGSKVKAYVQFRGRSIVFSDRGEVLLMRFAQRLEEF
                     GLPESMPKLEGKRMHIILTPKTKKN"
     gene            complement(3908..5839)
                     /gene="thrS"
                     /locus_tag="PK742_02015"
     CDS             complement(3908..5839)
                     /gene="thrS"
                     /locus_tag="PK742_02015"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQU75393.1"
                     /translation="MIRITFPDGAERQYEAGVSAMDIAKSISDGLARKVLSAKVNGQV
                     WDASRPINEDATLQLLTWDDKDGKNTFWHSSAHLMAEALESLYPGVKFGIGPPVDNGF
                     YYDIDLGGKTISAEDLVAVEKKMKELASQKNPYIRKEVSKADAISYFTEKGDQYKLEL
                     ISELEDGKITFYEQGNFTDLCRGPHIPDTGFIKAIKLTNIAGAYWRGSEKNPMLTRIY
                     GVTFPKQSELDEYLTMMEEAKKRDHRKIGKELEIFTFDDDVGPGLPLWLPNGGVMIEQ
                     LEKLAKKSEQKAGYSRVKSPHIAKESMYLKSGHLPYYEDSMFPAMDIDGQKYYLKAMN
                     CPHHHKIFDAVPRSYKDLPLRLAEYGTCYRYEQSGELFGLMRVRSLQMNDAHIYCTEE
                     QFESEFRAVNDMYLRYFEIFGIDKYVMRFSTHAPEKLGQKYVDAPELWKKTEDMVRRV
                     LISSGIPYIEVADEAAFYGPKIDVQVWSAIGREFTLATNQVDFAQPLRFNLKYMNNNN
                     EPEIPICIHRAPLGTHERFIGFLIEHYAGNFPAWLAPVQVAVLPISDKFNDYAAGVVE
                     MLKKHEIRPVFDTRSEKIGKKIRDAETRKIPYMLIVGEKEAENGTVSVRKHGAGDQGS
                     VTAEQFIEQINLEIEPIFS"
     gene            complement(5843..6904)
                     /locus_tag="PK742_02020"
     CDS             complement(5843..6904)
                     /locus_tag="PK742_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU75394.1"
                     /translation="MFKNPRVILFSVLVVAALIVAYLLADRQGWFASESTDQDTLSAI
                     EQLTPEIASLSEQIRNAPSNAGLFYSRGNAYYDFGNLKYAEADYARAFELDSNNATYV
                     LGWSDCLFDMNEVSKAVEMLENYQLRIPGNPDIMFSLAVDYFLLPQPKYPQSIDLLNE
                     ILKQDIQNAPAYFYKGLVFKEWGDTTKAISSFQTCTEVDPDYYDAWLQLGLLYADKGD
                     PLAVNYFDNAIAVSDSSREAEYAKAKYFQDKGNLAEAIEYYRQMVIKDPQDADALYNL
                     ATIYFGVDSIVKADRYFDMAIKQSPAKAYAHYGKGLCSMKLGHNKEAVAYFQQAITLG
                     VDEETEAECNELIRQINGN"
     gene            6991..8100
                     /locus_tag="PK742_02025"
     CDS             6991..8100
                     /locus_tag="PK742_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75395.1"
                     /translation="MNGRLVLLFAGLLLLLSYGLQMGGKLVSEGKQEVVSEASEPAAS
                     LAFPEEVHLKNVRQLTFGGDNAEAYWSFDDQMLSFQYRNLSEGLDCDQIYWADAHGKT
                     FAPHMISNGKGRTTCAFFLPDNKHVLYASTFATADSCLHDPDRSHGYLWGVYPSYEIY
                     VSDLEGNIVSQLTDNNYYDAEATVSPKGDKIVFTSDRSGDLELWTMNLDGSDLKQITS
                     DLGYDGGAFFSPDGSKIVFRASRPSTPEAKKEYKDLLKTGYVRPNEMELFICNADGSD
                     LRQITHLGGANWAPFFHPSGNKIIFSSNHMTKRIPFNLFMINVDGTGLEQITYDEVFD
                     SFAMFSNDGKKIVFGSNRNNHGTRDTNLFMADWED"
     assembly_gap    8234..8333
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8512..9852
                     /locus_tag="PK742_02030"
     CDS             8512..9852
                     /locus_tag="PK742_02030"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQU75396.1"
                     /translation="MSTIIRNVRLVNEGQITETDVLIRNGRFEKIAPDISVPERAEEV
                     DASGLHMMPGIIDDQVHFREPGLTWKEDIAHGAKAAVAGGVTSFMEMPNVVPPSFTHE
                     LLEEKYAIAARNSVANYSFYLGTSHDNLEEIKRIDATSICGLKIFMGSSTGNLLVDDP
                     KILEEVFRNSPTLIATHCEHEPTVRANTEKYKAIYGDHIPVALHPVIRDEQACYLSSS
                     FAVDLAKKTGARLHILHISTAEELDLFDNNIPLEQKRITAEACVHHLYFDAGDYEELG
                     SRIKWNPAVKDARHKPQILQAVLDDRIDVIATDHAPHTLEEKSNPYTSCPSGAPMVQH
                     SINVMLSFYHQGRISLEQIAAKMSHDVARCFRMEDRGYIREGYHADFFLLDPDAPFTV
                     TKESLLYKCGWSPLEGFQMQGKVHQTYVNGQLVWNGIHVIESANGERLRFRRLA"
     gene            complement(10020..11939)
                     /gene="yidC"
                     /locus_tag="PK742_02035"
     CDS             complement(10020..11939)
                     /gene="yidC"
                     /locus_tag="PK742_02035"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQU75397.1"
                     /translation="MDRNTIIGFVLLGVLFIGYLTIQTRISQKEQIQQAILQDSIEQV
                     EKTRVADSLAKVQAQREAMLLEAAGTDVVIDSLNETERQALLDSITAVKAAIAERETE
                     QTVEESRGSLAAAFNGKEETFSIENEELIVTFSNKGGRIKEVKLKQFNDYHGKPVKLV
                     GGDDNRFSYSFFYDTDKRVSTDELYFTAVPGKDGKSVTYVLDAGDGRVMQQQYAFNDT
                     SYLLDYSISMQGFDKLIPGNNPAVVLNWDNSMRMQEKNLDYERQYSKFFWKYMSGDLE
                     DQSSNGEEKFDATISWVCNQQQFFNTTLIARKGFDNVGSVHVVEGEDSTTMVKRCETE
                     LYIPYAGQPNYQFDMQWYFAPNHFRSLKELDLGLEDIVPTGTGLSKFINRGVIIPLFH
                     WLGGFISNYGLVILLMTLIIKLALSPLTYRSYLSMAKMRVLQPEIAEMKEKYKDDQQK
                     QGAEQLKLFRKAGVNPLGGCIPTLLSMPILISLFRFFPGAIELRGKSFLWADDLSTYD
                     DLIHWGFDLPFLGSHLSIFCVLMTVSSILYVRMNMSNATGMAKEMQFMQYIFPIFFLF
                     FLNNYPAGLTFYYFVSNMTTFGQQFAIRRFFINDEAIHAKIQENKAKPTKKSRFAQRL
                     EDAMKQQQEMQKRKK"
     gene            complement(12058..13683)
                     /locus_tag="PK742_02040"
     CDS             complement(12058..13683)
                     /locus_tag="PK742_02040"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQU75398.1"
                     /translation="MKSNCKYIFVTGGVTSSLGKGIFAASLARLLQGHGFRVTIQKFD
                     PYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLGVDTSQSNNVTTGAVYQTVI
                     NKERAGDYLGKTVQVIPHITDELQRRMMLLGESGEYDIVITEIGGTVGDIESFPFVEA
                     VRQLKWKLGSTRCITVHLTLIPYLASAKELKTKPTQHSVKMLLESGLQADILVCRTEY
                     PLNRDIRRKLALFCNVNINAVIEAIDVDTIYDVPLSLLKEGLDDIVLSKLKLPKHGEP
                     DLTSWKEFLSRLKNPTNSVRIALVGKYVELQDAYKSILESFIHAGAANECKVEVVSIQ
                     SDHISAENVREKLKHMDGVLVAPGFGDRGIEGKIAAIKFVRENNIPFFGICLGMQCCV
                     IEFGRHVLKLKDAHSTEMNPKTKHPVIDKMEEQKNITEMGGTMRLGSYDCRLYKKTRS
                     YDIYKSQAITERHRHRFEFNNEYLKDYEAHGMMAAGVNPQQNLVEIVELKEHPWFIGV
                     QFHPELKSKVEKPHPLFVDFVRAAIQQKERKLK"
     gene            13919..16348
                     /locus_tag="PK742_02045"
     CDS             13919..16348
                     /locus_tag="PK742_02045"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU75399.1"
                     /translation="MKRIFFLIAGIWFADEAWSQASLEGMVQDTGGEAVPFANAVLYQ
                     QADSQMVKAEVTDMDGHFQLMNIPAGTYRLEVSFVGFSDYVSDNIILGEKEKRSLPLI
                     TMATDENVLDAVEIVSERSLIEIQPDKTVFNVDGSANATGNTALELLRKAPGVMVDNN
                     DNIVLMGKSGTVVYIDGKPSPLSGADLAAFLNALRSEEIDAIEIITNPSAKYDAEGDA
                     GIINIRLKKNKAFGTNANINLGYNQGFYAKYNGSLNVNYRNKFMNVFGGYNFNVGTWR
                     NTMDIYRIQSGQIFDASSVRTASGPFNGLKFGTDFFVGDKSTIGFLVNGGKNVNNWNS
                     LGVTPIYDLASGEQLTTLRAISDNDGYYFNSNYNMNYRYDDKNGITWNADVDFGQFEI
                     TNDTYQPNYYVDPLADTVTAANIFTFTAPTNIDIYSAKLDHERPFLGGKLGLGVKSNY
                     VTTNNTYTFYEVIDNEEILDPDRSNNFVYQENVQAAYFSFVRDIKKFGINLGLRVEQT
                     HSIGTLTSQQDLENDEVERKYTDFFPSGGLTYKLNDKNQLRLNYSRRIQRPNYDALNP
                     FEFKMDELTYRRGNPFLQPQYTNSVQLTHTFAYMLNTSLNYSYTRDFFSPLSDTTEGT
                     RTYLETVNLGYQRNIGLNISAPFSPVKWWSTYTNLGVYNLENGGQLDDGRDIKINVTS
                     FNVYHQSTFNVGPKTSLEVSGWFGSPSVWGAVFQTEANWSMDIGVQQKLLKDRGNLKL
                     AFTDVFHTAPWDAIQELPGLYMDLAGGWESQQIRLTFSYILGNQQVKAARNRKTGIED
                     ASGRVGSGDGQ"
     gene            16540..17157
                     /locus_tag="PK742_02050"
     CDS             16540..17157
                     /locus_tag="PK742_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75400.1"
                     /translation="MRSLILAASCLLFASCATTEKVYVDSYSAELICQASTDHYFDNG
                     TLSKCRLTEPAMLGGIVCDGWIHFNEDGCIDQCLLARPIPFSGLSVPVGSWLLFDTED
                     PDHIAVIMFPQDMVVEGVTVRGGVKIMTSFHQNGRLKGCFLREDQVIDGIPCKASVFQ
                     EVRFHENGQLESCELSDDATLGDMTLPAGDRIELDSSGQLILLPL"
     gene            17180..17674
                     /locus_tag="PK742_02055"
     CDS             17180..17674
                     /locus_tag="PK742_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU75401.1"
                     /translation="MVQLRRPGAEDITILATLANNRKVWENLRDHFPFPYLESDAREF
                     ISMCEEEDPPHTFAITCSGEFCGIAGFSRGINPDEAELGYWLGEPYWNRGITSEAVRQ
                     MVAYGKNELGLKCITAAVFEFNPVSSRILEHAGFIKTKQENNHFSIKNGQKVREIHYI
                     LKIN"
     gene            17707..18051
                     /locus_tag="PK742_02060"
     CDS             17707..18051
                     /locus_tag="PK742_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU75402.1"
                     /translation="MAKNVSVDLFFERLDHPLKREMLALRELLHKTDNRLKETIRWGA
                     PTFEVDGRIAALDLRSDQSVKLTFLTGAPSRAGKVLQQTENGNTAFLVSSIEELRSKE
                     ESLLHLVQEWVH"
     gene            18140..>18877
                     /locus_tag="PK742_02065"
     CDS             18140..>18877
                     /locus_tag="PK742_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HQU75403.1"
                     /translation="MIRSYLLFACLCSLSFSLHAQTPELTAWIYNTTGQTGSHYEVGT
                     YTPFSDDALANVQEVEYSDDNVYVHSTGVPAYPTGPFQDGNPSYASELEYLFRIPRDP
                     EPNTGTLTEPGLGHIGVWINGVPVYNYSDAMSYNGMDIWHRNAVVFENAGFDCSKGHP
                     APIMGAPGPDIPGMYHHHQNPSPFSAATAPLSDVCESYPANGLYIPDAGQHSPLLGYA
                     FDGYPIYGAYAYGNTDGSGGIRRMESGW"
BASE COUNT         4629 a         4804 c         4524 g         4820 t
ORIGIN      
        1 aggttggaga aatggagtct tacgacttcg gaaaatttgc ctggatactg gatccggagg
       61 ggaacaagat agaactgtgg gaaccctcgc cgggcaacga atttcccgga gggatggaca
      121 tggaatgact acttcctggt gatcagtccg atcaggaaga gaaagaccac cgctcctacc
      181 accgagcttg caaggctacc tgcaagtccg ctggaagcta tgttcatctg tccgaatatc
      241 cagccgccta acacagcgcc cacgataccg acgatgatgt ttcccagcca tccgaagcct
      301 tcaccgcgaa tgagtttgcc gctgatccag ccggctgcgg caccaatgag aataaaatac
      361 agaaagctca tatgtttggt ttttcctgat caaaaaactg aagtttcagc agtcctgaaa
      421 aaatacctcc atcgatatta gccaactctt cgaagttgcc tttctcagcg atcctgccgt
      481 cttccagtac aaatatatgg tcggcatgac gaatggtcga taatctgtgt gcgatgatga
      541 tggtggtacg gtctttcatc agcgtatcca atgcatcctg caccagcctc tcgctttccg
      601 catcgagcga actggtggct tcatccagga tgaggatggc cggatctttc aggatggcac
      661 gggcaatggc aatgcgctgc cgctgaccac cactcagttt cacccctcgt tctcctacta
      721 ccgtatcaag cttttccgga aaaccggaga tgaattccca ggcatgtgcc tttctggccg
      781 ccgcttcgat ctcagaatcc gtagcaccag gtttgccata ggaaatattt tcccggatgg
      841 aaccgccaaa caggatgacc tcctgcggca ccagtgccat gttgttgcgc agatgtttta
      901 gatcgtagtc tgcatatggt cttccatcta ccgagatact tcctgacgaa ggctgataga
      961 atcccagcac cagttgagcg atggtactct ttcccgctcc actgtggcct gccagggcaa
     1021 tgcgttcgcc ggaacggatc tgcatatcaa tgcctttcag cacttccaca tccgttcggg
     1081 tgggatagga aaaatgtacg ttggaaaaag ttacttctcc cctgacaggt gtttcttctt
     1141 tcgggtcctg caggtccacc tcactgtctg tatcaatgat ctccatgacc cgttcgctgg
     1201 caccaacggt cttctgcaac ctgccgtaca gatcgccgat accacccact gcaccaccaa
     1261 tgaatccggt atataacatg aaggctgtga gtgtacccgc cgtcacctct ccttctttca
     1321 tgagcacagc accgtaccac aacaccagca cgaatccgcc gaacaaacca aagatgagaa
     1381 aggtggtgaa ggccccgcgg ttgaccgccc cgcggatacc tgcatgcacc acgcgaccta
     1441 aaaaaccttt gtaacggatg gattcgaacc actcgttggc aaatgctttc acagtagata
     1501 cattctgcag ggtctcttcc agcacgatat tactttctgc cagtgcatcc tgcctttcct
     1561 tgctgagcct tctgatgaat ttaccataaa gcatggccag gatgatgagc accggaaaag
     1621 tactcagcat aagcagggtg agtttggcgg aaatgaaaaa tatgaagcct accccgaata
     1681 caatggtcac gatctgacgg atgaattccg gaagcgtcat gctgagcata tcctgcaact
     1741 ggtcgatgtc gttcgtactg cgactggtca gctctcctac tcttctttct tcaaagaacg
     1801 gaatggacag ggtaatgagt ttgtcgtaca ggagctgtcg cacatcggcc atcgacttct
     1861 cggtgaccac tgcgaacaga tagacccgga agaaactgat gcctgcttgt acgaacagga
     1921 tccccaccag caccagggcc accgtgttgg tattcgtcag ccagtcgccc tccacttcca
     1981 cgcctgccgt gtcgatgagt tttcccatga aaaagggaaa ggtcatggtg gtgaaggaag
     2041 agatcagcag gaagctcata ccggctatga acatgcctct gtacggacgt agaaaggaga
     2101 agatgcgcat agcggtgcgc agactggaag cgtttagtcg cttcttgtca cgctcctcta
     2161 ccggggcttt tcttctgctt cgggggctgc tcattgcgga ggctcaaaat taggtttaat
     2221 attatggacg aaccattcat ccattcattg ccatgttgac gcattttgat atcatactgc
     2281 cggcctatcc cagaggattt catctgatcg acgaacaggt ctttggccag atagagctgc
     2341 ccgaaaccgg gctgctgcac attttcatcc accatacatc cgccggactt tgtatcaacg
     2401 aaaatgccga ccctactgtt cgcctggatt tcgagaccat tttcaacaag ctggttccgg
     2461 agggcttgcc ggaatacctg cataatctgg aaggcgatga cgacatgccc gcccatgtga
     2521 aatcggtttt atcaggtgca tccgtcagtt tgcccgttcg caacggccgt ccggtactcg
     2581 gtacctggca gggcatttat ctgtgcgagt tccgcaataa tggtggcaga agaaagctga
     2641 cggtcagtgt ggtggaataa caaaaaaccc cggaatcgct tccggggtct ctgttgtatt
     2701 gcaatgaatt acttcacctt gttgacgatc tcctcaaagg ccgccggatg gttcatggcc
     2761 agatcggcca gtgccttgcg gttgagttcg atacctgcct cttgcacctt gtggatgaaa
     2821 gtactgtagg tcattccgta aggacgaacg gccgcgttga tacgagcgat ccagatctga
     2881 cggaaatggg ctttcttatc cttccggcct acataggcat actgatgtgc tttctcaacg
     2941 gcattcttgg ctaccgtcca cacattcttt cttcttcccc agaagccttt cgcctgcttg
     3001 aggacctttt tacgacgagc cttagaggct gcatggttga ctgaccttgg catgatcttg
     3061 tttttacgct aatcccaacg ttcttttaat acgcggcgta tctgcttcgt gtaccacagt
     3121 ggtaccctgc aggcgcttct tgcgcttgtt gctcttcttg gtgaggatgt ggctgtgata
     3181 agccttgctc ctcttcagtt taccgcttcc tgtcagcttg aaacgcttct tggaactgcg
     3241 gtttggtttc atcttaggca tacgctcact atttcctgtt ttacaaacgg ggttgcaaag
     3301 atactaaata gtttttctta ctaaaaaggc ctgaacccta atttttcttg gtcttgggtg
     3361 tcaggatgat gtgcatccgt tttccctcca gtttgggcat ggattctggc aggccgaatt
     3421 cctccagacg ctgggcaaag cgcatcaaca gcacctctcc acggtctgaa aagacaatgg
     3481 aacgccccct gaactgcaca taagctttta ctttggatcc ctcctggagg aatttttctg
     3541 cgtgtttggt cttgaattca aagtcgtgct cgtcggtatt cgggccaaag cggatctctt
     3601 tcatttcctg cttcttgttc ttggccttga gctctttttc cttcttcttc ttctcgtaca
     3661 ggaacttctg gtaatcgaca atacgacata caggcgggtc tgcattgggg ctgatctcta
     3721 ccaggtcaag ctccatttcc tgagcaatgc ggtaggcttc ctctgttgaa taaacgccgg
     3781 cttctatgcc ttcacccacg agcctgacct taggcacgcg gatctctctg ttgatacggt
     3841 tggggttttg tttgactctt cctctgaaag gaggtctggg tcctcttctt tgcaagcgat
     3901 gtttttttta tgaaaaaatg ggttctattt ccaggttgat ctgttcaatg aattgttctg
     3961 ctgttacact gccctgatct cccgctccgt gtttcctgac agataccgtg ccattctctg
     4021 cttctttctc gcccacaatg agcatatagg gtatctttct ggtctctgca tccctgatct
     4081 tcttgcctat cttctctgag cgggtgtcaa agacagggcg aatttcgtgt tttttcagca
     4141 tttccaccac cccggctgca tagtcgttga acttgtcgct gatgggcagt acagccacct
     4201 ggaccggtgc gagccaggct gggaagttgc cggcatagtg ctcgatcaga aagccgatga
     4261 agcgttcatg agtgcccagg ggtgcccggt ggatgcaaat gggtatttcc ggttcattgt
     4321 tgttgttcat gtacttcagg ttgaagcgca ggggttgggc gaagtccacc tggttggtgg
     4381 ccagggtgaa ctcacggccg atggctgacc atacctgcac atcgatcttc ggtccgtaga
     4441 aagcggcttc atcggccacc tcgatatacg ggataccgga actgatcaac acccgcctga
     4501 ccatatcttc ggtcttcttc catagctcag gggcgtccac atatttctgc ccgagttttt
     4561 ccggtgcatg ggtgctgaag cgcatcacat acttatcgat cccgaagatc tcaaagtaac
     4621 gcaggtacat atcattgacc gccctgaact ctgattcaaa ttgctcttcg gtacaatata
     4681 tatgtgcatc gttcatctgc agactgcgca cccgcatgag cccgaacagc tcgccgctct
     4741 gctcatagcg gtaacaggtg ccgtactctg ccagacgcag cggcaggtcc ttgtagcttc
     4801 ttggaacagc gtcgaagatc ttgtggtggt gcggacagtt catcgccttg aggtaatact
     4861 tctgtccgtc gatgtccata gccgggaaca tactgtcttc gtaatacggc aggtgcccgc
     4921 ttttcagata catgctttct ttggcaatgt gcggcgactt caccctgctg tatcctgctt
     4981 tctgctccga ctttttggcc agtttctcca gctgttctat catgacccca ccattgggta
     5041 accacagtgg cagaccgggc cctacgtcat catcgaaagt gaagatctcc agttccttac
     5101 cgatcttccg gtggtcgcgt ttctttgctt cctccatcat ggtcaggtac tcatccagct
     5161 cagactgctt cgggaaggtc accccataga tgcgggtcag catcgggttc ttctcacttc
     5221 ccctccagta agccccggca atattggtca gcttgatggc cttgatgaaa ccggtgtcgg
     5281 gtatatgcgg accacggcag aggtcggtga aattgccctg ttcataaaag gtgatcttgc
     5341 catcttccag ttcgctgatg agctccagct tgtactgatc gcccttttcg gtgaagtagg
     5401 aaatggcatc ggctttcgat acctctttcc ggatataggg attcttctgc gaagccagct
     5461 ccttcatctt cttctccaca gccacgaggt cttcggcaga aatggtctta ccacccagat
     5521 cgatatcgta atagaatccg ttatcgaccg gcggacctat accaaatttc acacccgggt
     5581 agaggctttc cagtgcctct gccatcaggt gtgcagaaga gtgccagaag gtgttcttcc
     5641 cgtctttatc gtcccatgtg agcagctgga gcgtagcatc ttcgttgatg ggccgggaag
     5701 catcccagac ctgtccgttc acttttgcgc tcagcacctt gcgggcaagt ccgtcgctga
     5761 tggacttcgc aatgtccata gcactgacac cggcttcata ctgccgctct gctccatcgg
     5821 gaaatgtgat cctgatcatg tattaattac cgtttatctg tctgattagt tcgttgcact
     5881 cagcttctgt ctcttcatcg accccgaggg tgatggcctg ctgaaaatag gcaactgctt
     5941 ccttattgtg ccccagtttc atggaacaca ggcctttgcc atagtgtgca taggctttgg
     6001 cgggcgattg tttgatggcc atatcaaagt acctgtcggc cttgacaatg gaatcgacac
     6061 caaaataaat agtggccaga ttgtacagag catctgcatc ctgcgggtct ttgatgacca
     6121 tctgccggta gtattcaata gcttctgcca gatttccctt atcctgaaaa tatttggctt
     6181 tggcatattc cgcctcccgg ctgctgtcgc tcacggcgat agcgttgtca aaataattga
     6241 ccgccagtgg atcgccctta tcggcataca gcaagcccag ctggagccat gcatcgtagt
     6301 aatccggatc aacctccgtg caggtctgga aactggagat ggctttggtg gtgtctcccc
     6361 attccttgaa aaccagcccc ttgtaaaaat aggccggagc attttgaata tcctgtttga
     6421 gtatctcatt gagcagatcg atggactgcg gatattttgg ttggggaagg aggaaataat
     6481 cgaccgcaag agaaaacatg atatccggat ttcccggaat gcgcagttga taattctcca
     6541 gcatttcaac ggccttactg acttcgttca tatcgaacag acagtctgac catcccagca
     6601 cgtaggtagc attattggaa tcaagttcga aagccctggc ataatctgcc tcggcatact
     6661 tcaggttgcc gaagtcataa taagcattac cgcggctgta gaacagtccg gcgttggaag
     6721 gtgcattcct gatctgttct gataagctgg cgatctccgg ggtcagttgt tcaatggccg
     6781 acagggtgtc ctgatcagta ctctccgagg caaaccatcc ctgcctgtct gcaaggagat
     6841 aagccacgat cagggcggca accaccagta cagagaacag tatgacccgc gggttcttaa
     6901 acatggcgca aaggtacgga taaagccgat ttcaagtcat gaacgacaag gcttcctttc
     6961 gtgttgtagc tttgcagcca aattggaaaa atgaacggca gacttgtatt actttttgca
     7021 ggacttttat tgttgctgag ctatggcctg cagatgggag gaaaactggt ttctgaggga
     7081 aaacaggaag tagtatctga agcatcggaa ccggcagcat ccctggcttt tccggaggaa
     7141 gtacacctga aaaatgtcag acaactgact tttggcggcg ataatgcaga ggcttactgg
     7201 agttttgatg accagatgtt gtcgttccag taccgcaacc tgtctgaagg gctggactgt
     7261 gatcagatct attgggcaga tgctcatggc aagaccttcg caccgcacat gatcagcaat
     7321 ggaaaaggaa gaaccacctg tgcctttttc ctgcctgaca acaagcatgt actgtatgct
     7381 tccacctttg ctaccgcaga ttcctgcctg catgatccgg acaggagtca tggctacctg
     7441 tggggtgttt atccgagtta tgagatctat gtgagcgacc tggaaggaaa tattgtcagc
     7501 cagctgactg acaacaacta ttatgatgca gaggcgaccg tatctcccaa aggagataag
     7561 atcgttttca ccagcgaccg cagcggagat ctggaacttt ggaccatgaa cctggatggc
     7621 agcgacctga agcagatcac cagtgacctg ggatatgacg gtggagcctt cttttcgccc
     7681 gacggatcga agatcgtgtt ccgggccagc agaccgtcta ctcccgaagc aaaaaaagaa
     7741 tacaaagacc tgctgaaaac aggatatgtg cgtccgaatg agatggaatt gttcatctgc
     7801 aatgccgatg gttcagacct tcgtcagatc actcatctgg gaggcgccaa ctgggcacct
     7861 ttctttcatc catccgggaa caagatcatc ttctcttcca accacatgac caagcgcatt
     7921 ccattcaacc tcttcatgat caatgtggat ggcacagggc tggaacagat cacctacgat
     7981 gaagtgttcg acagctttgc catgttcagc aatgatggca aaaagatcgt gttcggatcc
     8041 aacagaaata accatggcac ccgtgatacc aacctgttca tggccgactg ggaagactag
     8101 ggaaaggttg ttggttttta gttattggtt attggcagtt gcttaatagt tgacgtagtg
     8161 agttattcgt tgaatgcagc acggatcggg tccatacctg aggtccatgt atcttgcatg
     8221 tcctttttca ctannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgacta
     8341 ctgactactg actactgact actgactact gactactgac tactgactac tgactgctca
     8401 ctgctcactg ctgactgctc actacccact acccactacc cactacccaa tactcaatac
     8461 tcaatactca atactcaata cccaataccc aataccaact atctttacaa tatgtccacc
     8521 atcattcgca atgtcagact ggtaaacgaa ggacagatca cagaaacaga tgtgctgatc
     8581 cgaaatggca gattcgagaa gatagcaccg gatatttctg tgccggaaag agcggaagaa
     8641 gtggatgcca gtggattgca tatgatgcct ggtatcattg acgaccaggt acactttcga
     8701 gaaccgggcc tgacctggaa ggaggatatt gctcacggag ccaaagcagc agtggcaggt
     8761 ggagtgacca gtttcatgga gatgcccaat gtggtaccgc cctccttcac ccatgaactg
     8821 ctggaagaaa aatatgccat tgccgccaga aactcagtag ccaactacag cttttacctc
     8881 ggcaccagtc acgataatct ggaagagatc aaacgcatag atgcgacttc catctgcgga
     8941 ctaaagatct tcatgggaag cagcaccggc aacctgctgg ttgatgaccc gaagatcctg
     9001 gaagaggtgt tccgcaacag cccgacactg atcgccacac actgtgaaca tgagccaacg
     9061 gtaagagcca acacagaaaa atacaaagcc atttacggcg atcatattcc tgttgcactt
     9121 catccggtga tccgtgatga acaggcctgt tacctttcgt cttcttttgc ggtggacctg
     9181 gctaaaaaaa ccggtgcaag gctgcacatc ctgcacatca gcaccgccga agaactggat
     9241 ctgtttgaca acaacattcc actggaacaa aagcgcatca cagcagaagc ctgtgtacac
     9301 catctgtatt tcgatgccgg cgattatgaa gaactgggtt cccggatcaa atggaacccc
     9361 gctgttaaag atgcacggca caaaccacag atcctgcagg ccgtgctgga tgatcgtatc
     9421 gatgtgatcg caacggatca tgcgccacat accctggaag aaaaatctaa tccttacact
     9481 tcatgcccgt caggcgcacc gatggtacag cacagcatca acgtgatgtt gtccttctat
     9541 caccagggca ggatatcgct ggaacagata gccgccaaga tgagtcatga tgtggcccgc
     9601 tgtttccgta tggaagaccg tggttacatt cgtgaaggtt atcatgcaga tttcttcctg
     9661 ctcgatccgg acgccccttt tacagtgacc aaagaaagcc tgctgtacaa atgcgggtgg
     9721 agtccattgg aaggatttca gatgcagggt aaggtgcatc agacctatgt gaacggacaa
     9781 ctggtctgga acggaatcca cgtgatcgaa tcagccaacg gagaaaggct gaggttcaga
     9841 aggctggcct gaaaaattcg gaaaccggaa tacggaatgg aattggccgt tggcaatgga
     9901 acaggatacg tactaacttg tttgcccaaa atttcaccac tcaccactca ccactcacca
     9961 ctcaccactc accactcacc actcactcct cactgctgac cgccaactgc caactaatcc
    10021 tacttcttcc tcttctgcat ttcctgctgc tgcttcatgg catcttccag acgttgtgca
    10081 aagcgtgatt tcttggtagg cttggccttg ttctcctgga tcttggcgtg gatggcctcg
    10141 tcgttgataa agaagcgacg gatggcgaat tgctgcccga aggtggtcat gttactcacg
    10201 aaataataga aggtcagtcc cgcaggataa ttattcagga agaacaggaa gaagatcggg
    10261 aagatgtact gcatgaactg catctccttg gccatacctg tggcgttgct catgttcata
    10321 cgcacataca ggatggagga aacggtcatc agcacacaga atatactcag gtgacttccc
    10381 agaaagggta aatcaaagcc ccagtgtatc agatcgtcat aggtactcag gtcatcggcc
    10441 cagaggaatg acttacctct cagttcgatg gctcccggga agaatcggaa cagcgagatc
    10501 aggatgggca tggacaagag cgtcgggata caacctccca gcggattcac acccgctttc
    10561 cggaagagct tgagctgctc tgccccctgc ttttgctgat catccttata cttttccttc
    10621 atctcggcga tctccggctg cagtacccgc atctttgcca tactgagata agagcggtag
    10681 gtaagcggcg acagggccag cttgatgatc agggtcatca acaggatgac cagaccataa
    10741 ttactgatga atccacccag ccagtggaac agcggaatga tgacgccccg gttgatgaat
    10801 tttgacaaac ctgttccggt tggaacgata tcttccagcc cgagatccag ttccttcaga
    10861 gagcggaaat ggttgggtgc aaaataccat tgcatatcga actgatagtt cggctgacca
    10921 gcatagggaa tgtacagttc agtttcgcag cgcttcacca tggtggtgct gtcttcacct
    10981 tcaaccacat gcacactgcc cacattatca aaaccttttc tggcgatcag ggtggtgttg
    11041 aagaattgct gctggttgca gacccaggaa atggtggcgt caaacttttc ttccccgttt
    11101 gacgattgat cttccaggtc gccgctcata tatttccaga agaacttgga atactgtctt
    11161 tcatagtcca ggttcttctc ctgcatgcgc atggagttat cccagttcaa cacaaccgcc
    11221 ggattattac ccggaatgag tttatcaaag ccctgcatgg aaatggagta atccagcagg
    11281 taactggtgt cgttgaatgc atactgttgc tgcatgacac gaccatcgcc ggcatcgagc
    11341 acataggtca cgcttttgcc atcctttccc ggaacagcag tgaaatacaa ttcatccgtg
    11401 ctgacacgct tgtcggtatc gtaaaagaat gaatagctga acctgttatc atccccgccc
    11461 accagtttca caggtttgcc atggtaatcg ttgaattgtt tgagttttac ttccttgatc
    11521 ctaccccctt tgttgctgaa ggtcacgatc agttcctcat tctcgatgga aaaagtctct
    11581 tcttttccat tgaatgctgc agcaaggcta ccccggcttt cttccaccgt ttgctctgtt
    11641 tcacgttcag caatagctgc ctttacagcc gtaatggaat caagcaatgc ctgtctttcc
    11701 gtttcgttca ggctgtctat cacgacgtct gttccggcag cctcgagcag catggcttct
    11761 ctttgtgcct gcaccttagc cagagaatct gccacacggg ttttctctac ctgttcgata
    11821 gaatcctgca gaatagcctg ttgtatctgt tctttctgag atatgcgggt ctggatggtc
    11881 agatacccga taaaaaggac gcccaggagg acaaatccga taatagtgtt acgatccatg
    11941 attcaggcgg caaaagtacg taatccggcg ggttgttagg ctaaaaatgc ctgaccaccc
    12001 tgacggggaa agctgctttt ctgtctgtca gacgaacggt tgacataaat atgctgccta
    12061 tttcagcttg cgctcctttt gctggatggc agcacgcaca aagtcaacga acagcggatg
    12121 tggcttctct accttgctct tgagctcggg atggaactgc acgccaatga accagggatg
    12181 ttctttcagt tctacgatct ccaccaggtt ctgctgcgga ttcacacctg cagccatcat
    12241 accatgcgct tcgtagtctt taaggtactc attattgaac tcaaagcgat ggcgatgccg
    12301 ttctgtgatg gcctgtgact tatagatgtc ataagacctg gtctttttgt acaagcgaca
    12361 gtcgtaagaa ccgaggcgca tggtaccgcc catttcggtg atattcttct gctcctccat
    12421 cttgtcgatg accggatgct tcgttttggg gttcatttcc gtactgtgtg catccttcag
    12481 cttgagcaca tgcctgccaa actcaatgac acagcactgc ataccgagac aaataccaaa
    12541 gaatggaata ttattctcac gaacgaattt aatggcagcg atcttacctt ctatgcctct
    12601 gtcgccaaaa cccggagcca ccagaacacc gtccatatgc tttagttttt cccgcacgtt
    12661 ttctgcactg atgtgatcac tctggatgct gaccacctcc accttacatt cattggcagc
    12721 tccggcatga atgaaacttt ccaggatgga tttgtaggcg tcctgcaact ccacatactt
    12781 tccaaccagg gcaatgcgca ctgaattggt aggattcttc agccgtgaca ggaattcctt
    12841 ccaggaggtc agatcgggct ctccgtgttt gggaagtttg agtttgctga gcacgatatc
    12901 atccagtcct tcctttagca ggctgagtgg tacatcatag atggtgtcca catcgatagc
    12961 ttctatgacc gcattgatat tgacgttgca gaacagcgcc agtttgcgcc gaatgtcacg
    13021 gttgagcgga tactccgtac ggcaaaccag aatatctgcc tgcaacccgc tttccagcag
    13081 catcttcaca ctgtgttgtg tgggcttggt cttgagttcc ttggcagatg cgaggtacgg
    13141 gatcagcgtc aggtgcaccg tgatacagcg ggtactgccc agcttccact tcagctgcct
    13201 gacggcttcc acaaagggaa agctttcaat atcaccgact gttccaccta tctctgtgat
    13261 gacaatgtca tactcaccac tttcacccag cagcatcatg cgtcgctgca attcatctgt
    13321 gatgtgggga atgacctgta cggtcttacc gagataatcg ccggcacgct ccttattgat
    13381 aacggtctga tacaccgcac ctgtggtcac gttattgctc tggctggtat ctacccccag
    13441 gaatctttca taatgaccca gatcgagatc tgtctctgca ccatcttccg tcacatagca
    13501 ttcgccatgc tcatacgggt tcagtgtgcc gggatcgaca ttgatgtaag ggtcgaattt
    13561 ctgtatcgtt acacggaagc catgcccctg gagcagtctg gccaatgagg cggcaaaaat
    13621 gcctttacca agtgaagagg taaccccacc cgtaacgaaa atgtatttac aattggattt
    13681 catatacaac gttacgggct ataaaggtaa gccaattttt gaggcagcca atcgtcactt
    13741 ctctgagact gtggataagc ctgtggatga tgtgattttc atggataaca ggcatgatat
    13801 catatctgct gcgttaaaag ctcttaaggc ctcctaaact ctggtaacaa atgcggtgat
    13861 aagcctttct tcctgataag tttgccccga tctcctgatc aacgacaacc caaccaacat
    13921 gaaacgaatt ttctttttga tcgcaggtat ctggtttgct gacgaagcat ggagccaggc
    13981 ttcactggag ggcatggtgc aggacaccgg tggcgaagca gttccttttg caaatgcagt
    14041 actgtatcag caagccgaca gccagatggt caaggcagaa gtgaccgata tggatggtca
    14101 ttttcagctg atgaacattc cggccggaac ctaccggctg gaagtgagct ttgtgggctt
    14161 ttcagattat gtgtctgaca atatcatact tggtgaaaaa gaaaaacgct ctctgccact
    14221 catcaccatg gccactgatg aaaatgtact cgatgcggtg gagatcgtca gtgagcgctc
    14281 tcttattgag atccagccag acaagaccgt attcaatgtg gatggcagtg ccaacgcaac
    14341 cggaaacacg gccctggaac tattgcgcaa ggcacccggt gtgatggttg acaacaatga
    14401 caacatcgtg ctgatgggca agagcggaac agttgtttat attgacggca aaccctctcc
    14461 gctcagcggt gcagatctgg ctgcttttct gaatgccctt cgctcagaag agatcgatgc
    14521 cattgagatc atcaccaacc cgtctgccaa atatgatgca gaaggcgatg ccggtatcat
    14581 caacatccgt ctgaaaaaga acaaggcttt cggcaccaac gccaacatca acctgggcta
    14641 taaccagggt ttctacgcca aatacaatgg ctctctcaat gttaactacc gaaacaaatt
    14701 catgaatgtg tttggtggtt ataattttaa tgtgggcacc tggcggaaca caatggatat
    14761 ttaccgcatt cagagcgggc agatctttga tgccagttct gtgcgcacag cttccggacc
    14821 tttcaacgga ctgaagttcg gaacagattt tttcgtggga gacaaaagca ccatcggctt
    14881 cctggtgaat ggtggtaaga atgtcaacaa ctggaacagt ctgggtgtca ctccgatcta
    14941 tgatctggca agcggcgaac aactgaccac cctgagggcc atcagcgata acgatggcta
    15001 ctacttcaat tccaactata acatgaatta caggtatgat gacaaaaacg gaattacctg
    15061 gaatgctgat gtggacttcg gtcagttcga gatcacaaat gatacgtatc agccgaacta
    15121 ctacgtagat ccgttggctg atacagttac cgcagcaaac atttttacct tcaccgcacc
    15181 aactaacatc gatatctact cagccaaact cgatcatgag aggcctttcc tgggtggaaa
    15241 actggggctg ggtgtgaagt ccaactatgt gaccacaaac aacacctata ccttctatga
    15301 ggtcattgac aatgaagaga tcctggatcc cgacagaagc aacaacttcg tctatcagga
    15361 aaatgtacag gcagcttact tcagttttgt acgtgatatc aagaagttcg gtatcaacct
    15421 cggtctgcgg gtagaacaaa cccattccat cggtacactg accagccagc aggatctgga
    15481 aaatgatgag gtagaaagaa aatacaccga cttcttccct tccggcggat taacctataa
    15541 gctgaatgac aagaaccagc tcaggctgaa ttacagcaga aggatccagc gcccgaatta
    15601 cgatgccctg aatccattcg aattcaaaat ggatgaactg acctacagaa ggggtaaccc
    15661 gttcctgcaa cctcagtaca ccaacagtgt gcagcttaca cacaccttcg cgtacatgct
    15721 gaacaccagt ctgaattaca gttataccag agattttttc agtccgctga gcgatactac
    15781 cgaaggaacc cgcacctatc tggaaacggt aaatctcggc taccagcgca acatcggact
    15841 gaacatcagt gcaccattca gtcctgtcaa atggtggagt acctacacca atctcggtgt
    15901 atataacctg gagaacggcg gacagctgga cgacggccgg gacataaaaa taaatgttac
    15961 ttcttttaat gtatatcatc agagtacttt caatgtaggc ccgaaaacct cgctggaagt
    16021 atccggctgg ttcggatcac ccagtgtctg gggtgcggta ttccagacag aagccaactg
    16081 gagtatggat attggtgtgc aacagaagct gctgaaggac agaggcaacc tcaaactggc
    16141 attcaccgat gtattccata cggcaccctg ggatgccatt caggaacttc cgggtctgta
    16201 tatggacctg gcaggaggat gggaaagcca gcagatccgg ctcaccttca gctacatttt
    16261 gggtaaccag caggtcaagg cagcccgcaa tcggaaaacg ggaatcgaag atgccagcgg
    16321 ccgtgttggc agcggcgacg gacaataaat gattcgatcg gtttagtaaa caaggcaggc
    16381 agcaatgtct gctttttttt atggtaaaca gcccacacta aacgttcatc atacacgatc
    16441 aattcatttg gctgtctgat ctcaggtatt gacaacaatc accaaaaaaa gtgcaacacc
    16501 tgctcttgct catcgtacct ttatacaagg aggtatatca tgagatcctt aattctagct
    16561 gcttcctgcc tgctgtttgc ttcctgcgcc acaacagaga aagtgtatgt ggattcttat
    16621 tcggctgaac ttatctgtca ggcctcaact gaccactatt tcgataacgg caccttatcc
    16681 aaatgcagac tgacagagcc cgccatgctg ggaggcatcg tttgtgatgg ctggatccat
    16741 ttcaatgagg atggttgtat cgatcagtgt ttgctggcca gacccattcc cttcagtggt
    16801 ctgtcagttc ctgttggatc ctggttgttg tttgacacag aagatccgga tcacattgct
    16861 gtcatcatgt ttccacaaga tatggtggtg gaaggggtta ccgttcgcgg aggagtaaag
    16921 atcatgacgt ccttccatca gaacggccgg ctgaagggat gcttccttcg agaagatcag
    16981 gtgatagacg gtattccatg caaggcatct gtttttcagg aagtgcgctt tcatgaaaat
    17041 ggtcaattgg aatcatgcga actgagtgat gatgcaacgc ttggcgatat gaccctgcct
    17101 gccggcgatc gtatcgaact ggacagcagc ggacagctca ttcttctgcc gctgtgaacc
    17161 ggaattaata acattgtaca tggttcagct gcgaagaccc ggagcggagg atattacaat
    17221 actggctacc ctggccaata accggaaggt atgggaaaac ctgcgtgatc actttccctt
    17281 cccatacttg gaaagcgatg cacgggagtt catcagcatg tgcgaggaag aagatcctcc
    17341 ccacaccttt gccattacct gtagcgggga attttgcggc attgccggat tcagtcgggg
    17401 aatcaaccct gatgaagctg aattaggcta ctggctgggt gaaccctact ggaacagggg
    17461 aatcacctca gaagcggtca gacaaatggt ggcgtatgga aaaaatgaac ttgggctgaa
    17521 atgcatcact gctgcagtct ttgaattcaa tcccgtttct tccaggatcc tcgaacatgc
    17581 cgggtttatc aaaaccaaac aagaaaataa tcatttttcc ataaaaaatg gccaaaaagt
    17641 gcgtgaaatt cattatatat tgaaaataaa ttaggaattt acaataatta tgtgtatatt
    17701 ttgcgcatgg ctaaaaatgt ttctgttgac ctgttctttg aaaggcttga tcatcccctg
    17761 aagcgggaga tgctggcatt gcgtgaactg ttacacaaga ccgacaacag gctgaaggag
    17821 accatccgct ggggtgctcc cacttttgaa gtggatggcc ggatcgcagc actggatctg
    17881 aggagtgatc agtctgtcaa gctgaccttc ctgaccggtg ctccgtcacg ggcaggaaaa
    17941 gtgcttcagc aaaccgagaa tggtaatacc gcttttctgg tctcttccat tgaagaactt
    18001 cgctcgaagg aggaatccct tcttcacctg gtacaggaat gggtgcatta agttgttcac
    18061 tgaaatagaa cgtgaataaa aaacagtcca ttgttaaacc ttgggctgac tgggctatct
    18121 tataccccaa acctgtatca tgatccgttc ttatctgctg tttgcctgtt tatgttcact
    18181 gtccttttca ttgcatgccc agacgcctga actcacagcc tggatataca ataccaccgg
    18241 acagaccggc agccattatg aagtgggtac ctatactcct ttctctgatg atgcattagc
    18301 caatgtgcag gaagtggagt acagcgatga taatgtgtat gtgcacagca ccggggtacc
    18361 tgcctatcct accgggccat tccaggatgg aaatccctcc tatgcatctg aactggaata
    18421 cctgtttcgc attcccagag atccggaacc gaataccgga acgcttacag aacccgggct
    18481 cgggcatatc ggagtctgga tcaatggcgt gcccgtatat aattattctg atgccatgtc
    18541 atacaacggt atggacatct ggcacaggaa tgcggtggtg ttcgagaacg ccggattcga
    18601 ttgcagtaaa ggtcacccgg caccgatcat gggtgctccc ggtccggata ttcccggcat
    18661 gtaccaccat caccagaacc cttctccttt ctctgctgcc actgccccac tttctgatgt
    18721 ttgcgaatcc tatcccgcaa acggcttgta tattccggac gccggacaac attccccttt
    18781 gctgggctat gcctttgatg gttatccgat ctacggtgcc tatgcttatg gcaacaccga
    18841 tggcagcggt gggatcagac ggatggaatc aggctgg
//
LOCUS       DAOMAX010000017        18523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_310_length_18523_cov_4.461555, whole genome shotgun sequence.
ACCESSION   DAOMAX010000017 DAOMAX010000000
VERSION     DAOMAX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_18523_cov_4.461555"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3339)
                     /locus_tag="PK742_02070"
     CDS             complement(<1..3339)
                     /locus_tag="PK742_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75404.1"
                     /translation="MNSRSDVRWRWLFLALAGFFPLLVSGQGTQIPFGQNRVQYKEFT
                     WQFYESDHFRVYFNQGGQNLGRFTAQIAENDLDEIESLLDYKLSSRPDIIVYNNISDF
                     NQSNFAIGNEALYNIGGRTKIIGNKLFVYFDGNHQHLRTQIREGVGKILVNNMVFGGN
                     LQEIVQNAVLLNLPEWFVEGLVSYIGEEWNADLDNQLRDGILSGRYEQFTRLTGEDAR
                     FAGHSFWYFIAEKYSPGDIPNLLYLIRINRSLENGFLFVLGTSLNQTIEEWYSYYRDR
                     YTAELRGKVNPDETEEMARKTRKNLVYHSPVISPNGQDVAYLSDDIGKFRIHLQEAGE
                     SKAAVIKKGGMRTRTRAIDLSYPLVAWSPDGKILAAIYEKRDKTVLFTYNTDDKKKET
                     KDISRFQRVLDMSFGKNNNMLVLSVMNNGQSDIYTYFINNARVEQITNDYYDDLSPRY
                     IKTDNGYEGILFASNRDDKRLERTRNIDSILPVGNNDLYFYKYDKKSKDLVQVTHTRN
                     ISETAPIAFNDTYFAFLSDYNGIRNRFAGYLDTIYDHTNTILYLSDSVVVNPTWDISE
                     VYKREDFDSLKYVEIFRDTAYTFPVTNYNTNILSHDISLRTGKLLEVFQESGRNQFYF
                     ARVPASIGGKDMPRLEKTGYRQYEERKQQEKVVEEVTEIPSLREEEKPSSDALTTDSL
                     REEQRKGMYFQTPFDVPEVLLEEPVIVLDEADKKDINNLVSEGFRQSRIQAYKLRFSS
                     EYVQTQLDNSLLINPYENFNATGGSFSQPPLSGLMTVSISDLFEDYRFTGGLRFPTSF
                     SGGEYFLRYEDVSKRIDKSLIWYYQHERAQYSFQPAWFPPVEAKPRTTIVEAAAKYPF
                     DVIRSLRASAAYRADKVIFLASDTFSLNQTNYKEDWFSLRLEYVHDNTLPVMTNIMYG
                     MRYKVWVEMHKQFAVNLDDGLDVSLNDGYLGVLGFDVRHYQKIHRQFIWANRLAGGVS
                     FGNQKLIYYLGGVDSWLFPQFNNTTEINQEQNYAFQTQATPVRGYEQNIRNGNAYAVF
                     NSELRMPVFTYLFKKPVKAEFLRNLQLVGFFDAGSAWEGFSPFEQDNPYNTVTFGQAP
                     LSVDVNFFRDPIVAGTGIGLRTTLF"
     gene            3419..4072
                     /locus_tag="PK742_02075"
     CDS             3419..4072
                     /locus_tag="PK742_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU75405.1"
                     /translation="MSGIAVFPFNPWQENTYLIYDANGIAAVIDPGCYSREEQQELRS
                     FMEAQSLQPLHLLNTHCHIDHVLGNAFVADTWQLDLAMHQGEIPVLKAVDTYADSMGI
                     QYQRSPDATLLLNEGDVVEVGSIRLEVLFTPGHSPASICFYNRAEGYVIGGDVLFLDS
                     IGRTDLPGGDMDTLLDSIRNKIFPLPDDTIVYPGHGPSTTVGREKKHNPFLNGRYQP"
     gene            complement(4063..5931)
                     /gene="htpG"
                     /locus_tag="PK742_02080"
     CDS             complement(4063..5931)
                     /gene="htpG"
                     /locus_tag="PK742_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQU75406.1"
                     /translation="MSEKGTIAVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKL
                     KKLSSLGEATGELGDISIEVIVNKKEGTLTISDKGIGMTADEVRRYINQVAFSSAQEF
                     LDKYKGVEDGKTIIGHFGLGFYSAFMVADHVTIRTRSWKNEPAVQWSCDGSPEYTLEE
                     TEKESRGTDIILHINGESKEFLRKEKIDELLNKYCRFLPVPVVFGTKEETIEVEGKEK
                     KNKVPNVVNNTHPAWTRKPAELEDEDYRKFYQELYPYSSAPLFWIHLNVDYPFNLTGV
                     LYFPKIKQNYEVQKNKIQLYCNQVFVTDEVKEIVPEWLTLLHGVIDSPDIPLNVSRSY
                     LQSDSNVKKINNYITKKVAERLEKMFTKEREEFEKQWENISVIVKYGMLTDEKFYDKA
                     SKFVLLQNTDGKYFTVEEYKEHIRELQTDKHDALTVLYSQNPEEHHVYIDAAKARGYD
                     VIQVDTIIDNHFISFLEGKLEKVQFKRVDADTADKLIDKDETTESVLTEEESKQVQEL
                     FEQAADQQAATVVLTPLSPEDNPVAITRPEFMRRMKEMSGYNGMDFAASMPDQYNLVV
                     NTNHPLIGALLKKEEAAQKEAVRQLQDLALLSQGMLKGADLSAFVKRSFGLIGDQG"
     gene            6059..7861
                     /locus_tag="PK742_02085"
     CDS             6059..7861
                     /locus_tag="PK742_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75407.1"
                     /translation="MARTSSASKQDQKQRIADLREKINDCYVSTITDIERYAKEIIEL
                     ATAVKDRNAEGLAFLDLSFYHCSSTNEYKKSLDCAQRGIDLLSGAQRTYYLPYYHLNR
                     GRCFQFMGDQIRAQEEYLDAISILEKRKNRTVEETRWMGSSYYNAYILFNQGGMEFSR
                     EDFLERAYLLYQQIDDKPGLSNCYNSYASIHYKKKELPETLENLEQAYKLSSEMQAYT
                     HMSIFSANIGLVHAQLGDVSKFREYFELCSKHTRKTKSRFHEAHMNGQMGEALFFLGM
                     YDESLERLATAEEIFSELGAKSSLTEILRKRSEIYALQDNYRLAYEYHTRYAELIRER
                     FNEEKATAIMRARMVYEIEEKENEAAILKEKNEEIQQYMNKLQASNNELQQFAYVVSH
                     DLREPLRTISTYIKLLEKNMTGRLDREEQEFIDFVVNGTRNMNELISDLLTFSKISYI
                     TETEQVGLQQLVQSTANEIVLHYLPAKDPVIHIGKLPVIEANRTHMKQLFGNLISNAI
                     KYNKHVQPEISIQCEDTGKMYTITVSDNGIGIAPEFRERVFQIFQRLHSKADYHGTGV
                     GLAICKKIVDNMGGQITLRGNNRKGSDFIFTLPK"
     gene            7858..8316
                     /locus_tag="PK742_02090"
     CDS             7858..8316
                     /locus_tag="PK742_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HQU75408.1"
                     /translation="MRYLLLCCSILMAAACSVQSPQYKGYSPFTIDKEEAGPVIHTGV
                     LLHNPNPIGVSLRNAELKVTVNSVELGDISLDESVRIKRKQDFMLPVTFRTTYSSLSG
                     AALANLPAILSGSPIPLTVEGELRVRKWLFFHKTFAVDYQDKIDLKDIQL"
     gene            complement(8313..8846)
                     /locus_tag="PK742_02095"
     CDS             complement(8313..8846)
                     /locus_tag="PK742_02095"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HQU75409.1"
                     /translation="MNISRPETMRYPEGLSWYVVYARSRSEKKVAERLERAGITCFLP
                     LLKVMRQWSDRRKMVEVPMFSGYLFVHTEPACFADVRMVEGVVNFVRSGGRNAIVPED
                     QIRTIQLFVETGLPGESGPDDLIPGERVQVTFGPLAGCTGELLEVRNQRRFVVRIESI
                     RQALIITLPTGYLERIN"
     gene            complement(8843..9427)
                     /locus_tag="PK742_02100"
     CDS             complement(8843..9427)
                     /locus_tag="PK742_02100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQU75410.1"
                     /translation="MEELKYRIHPTAVVDPGCTIGAGTSIWHFCHLMPGCTIGKNCSL
                     GQNVVVMPDVILGNGVKVQNNVSLYTGVICEDDVFLGPSMVFTNVLNPRAAVSRKDEY
                     RTTLVRKGATIGANATIICGITIGAYAFIGAGAVVTKDVLPYALVIGNPAMQTGWMSE
                     AGHKLEFSESGHAVCSGTGQQYVLKDGRVERLAV"
     gene            9480..10946
                     /locus_tag="PK742_02105"
     CDS             9480..10946
                     /locus_tag="PK742_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75411.1"
                     /translation="MKKIRLSLLLPVIALSACRTGFDGNLLDNQAPDTHTVADTIIRF
                     GDDRLESEVVVRWWGDDPDGVVAGYEFTFGDPYSLMADWQFTERQDSTFILAPPAGTD
                     TLDFYFSVRAIDQEGLRDPSPATLAYPVKNSPPSVAFQPGLNQPVISFPALTFYWEGS
                     DPDGDANLNGYEVVWNDTTVVPVPLEAGAGSLLLVADDPAITSMTCNMYVNASATPLA
                     TSLSGMQAHSWNKLFIRAVDQSGAYSPWTASDSVYIKPVQSNILLVNAYTTASNPTDF
                     YGSHLVNNGFTVLDTIDLFETAGGNYTQQSADNLTQEKVFAMFDCLIWYSNSAESSLS
                     LGQRTTSEFFDKGGHLLMSVYVSGSFDPLSNFLDFTPVSSLVSPADTTLLLEAGALLT
                     PTDDAYPDLQATAIVGVVKPVNLQIGAAPVYEAALTAKDNATLTFSPWTGGAVVMARK
                     PAAGEADFILSTLELQKLDGLLNMDDFFNKIMTDAFGY"
     gene            10930..11517
                     /locus_tag="PK742_02110"
     CDS             10930..11517
                     /locus_tag="PK742_02110"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQU75412.1"
                     /translation="MHSDTEQKKLVVLASGNGSNAENLIRYFSGSGLAKVTCVVTDQP
                     GAGVIVKAGKLGVPVRVITRRMATDGPTMLHLLEQETPDLILLAGYLRLIPEPVVRTY
                     HDRIINIHPALLPAYGGKGMYGAHVHQAVFDNREKETGITIHLVNERYDEGRILLQRS
                     TGIEPDDTPDSIAGKVHALEMEWYPITVQEYLSAG"
     gene            11609..15103
                     /locus_tag="PK742_02115"
     CDS             11609..15103
                     /locus_tag="PK742_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75413.1"
                     /translation="MRTNCFVRLVLLYMAICWLLPLHAQLRLTENKGQWTDQIRYRMD
                     LTYGNIYVEQSGLLFDMSDPEDLENIHYIKENSIPADPDDVIIRKHAWRMTPVGMQEQ
                     PAISTSQPSADYSNYFIGNDPDHWASSVRHFSSITIHDVFPHTNMQISSHADRPKYEL
                     TLYPGANLHNIAFVYSGLDSMHLKDGALVMQYSFGQVIDQQPVAWQIVHGKRVPVPCR
                     FILKGDTVGFVCPSGYQTDHPLIIDPEMVFASYSGSTSDNWGYTATYDQLGHMYGGGI
                     VFGSGYPTTTGAYDVSFSGGSIDMGLTKFTPDGSDLVWSTYLGGSGQDLPHSMVVNSA
                     FELLVYGTTSSPDFPMTPDSYDDVYDDGPTISLYSGFMTLTGADIIIAKLSDDGTMLE
                     GSTFMGGTDTDGFNLASATQYNYGDHSRGEIVVDAADNIYVASSTSSDDFPITAGTFD
                     DTYNGSQDAVVFCLNPDLSDLLWSTYLGGTSGDGAFGLKVSSSGQVVVTGGTASSGFP
                     VTAGTWTTSYAGGTADGFVSILSADGSSLVASTYAGTSQYDQSYFVELDEYDNVYITG
                     QSKGSFPVSAGVYSVTGGKQFIVKLNSSLTSVILSTVFGSGGSSVNISPSAFLVDECE
                     NIYVSGWGGSVNNSFNPATGNTTGLPTTADALDATTDGSDFYFFVLEKNMASLLYASF
                     FGGSPAEHVDGGTSRFDKEGTIYQAVCAGCGGSDGFPTTAGAWSEVNGSFNCNLGVGK
                     IAFNLAGVYAESDAEPSINGCAPFTVNFENLSSDAEEYIWDFGDGVGGSVEFEPIYTY
                     DSSGTYTVMLVVIDSSTCNIADTSYLTVTVYEDSIFADFDLISEQDCEALTAFFSDSS
                     DILDTTTWFWNFGDGGTSTLQNPEHTYTTPGTYEVMLVIYDPTSCNQYDTAYTTISFL
                     SEFSEDYSVSTTGCLPLVATFTSEFTGADSYTWNFGDGTSGTGPTIIHTYEETGTYLV
                     TLSVVSCGIEDITTFPVIVYGFPEAYFDSEPEIGLLNTPITFNNLSTGAVYYDWFFSD
                     GGYSDAENAVHTFTSLGGYEICLTVTNPADCEDTYCRSIIIENEGVIGVPSGFSPNND
                     GSNDVLYVRGFGIETMLFQVYNRWGQLVFSTTDQHIGWDGTYQGVPQEVEVYIYTLEA
                     EFMDGKTHREEGNITLIR"
     gene            15100..16104
                     /locus_tag="PK742_02120"
     CDS             15100..16104
                     /locus_tag="PK742_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU75414.1"
                     /translation="MKGIWYMILISFTGLTLQAQDVHFSQFYAAPLLVNPAQTGNFSG
                     TARVGANYRDQWGSITVPYRTFDLYSDAGIQPGKSVNRFGIGLCVLSDQAGDGVLRSN
                     RAQLSAAYHAMLNDDKTVRLSAGLSGGVVQRSLDFSKLTFDSQWNGYNFDSDLANQET
                     GFREQFIYPDFGTGILLTMTPLPYARYYVGASALHLNQPEESFFTDGSNQLGIRYLVT
                     GGAYFPVSEQVSMSPAAYLSMQKKALEVTTGTNFHIETDNGSNSAIFLLGLWYRYLDA
                     AWFMAGYVTGNLTVSASYDFNFSGLTAASNTLGGLELAIIYTFNKSQHKDPLGCPAFE
                     "
     gene            complement(16109..17980)
                     /locus_tag="PK742_02125"
     CDS             complement(16109..17980)
                     /locus_tag="PK742_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQU75415.1"
                     /translation="MVKKTQEATKILSDQSDADVFFRLSHDLLMVVGFDKQVKRLNPA
                     WEQVFGYALEELAAQNTRQTIHREDLPKTEQILQDMCDGKLQVIEYTQRMLTTGGECL
                     HYAWSLVSDSDKKVIYGIGRNVTSQVSLFSDMRRNEQRFNALFENTAVGILLLRTDGS
                     ILNINQQLLNRMGYHADAPPTRLKELIFEADLKDSMALFANLLEGKHQSTVAERRLKK
                     SNGDAAWSRVACTILEISPKEQLVMALVEDIEDERMNKKVLDATEKRFKAVFDSSPSG
                     IMITRNEGEILHANPAMAEMLQYDLEFLTGTNVFDYTYSEDRPDTMRMVQLLNNGQIA
                     EYDLEKRYVRKDGTTFWAKTWVSLMERSEGEVVRVAIVENIDRRKKAEQKLEEKNKEL
                     MEINQELEHFAYVASHDLQEPLRTVASFIQILEKKYSHLLDEDGQQFMGFVVDGAKRM
                     QALIRDLLEYSRINRFNTDYERVDLNEVLQMVNRALKEKIDSTDAVILAENLPSIQGN
                     KIQLTQIFQNLIDNAIKFKGEEAPEIIISVEELPGKWEIQVRDNGIGISQEYFQRIFI
                     IFQRLHTHEEYSGTGIGLALCKKIIERHGGEIWLDSAPGGGTTFTFTIAKNLIRAVS"
     gene            complement(18079..>18523)
                     /locus_tag="PK742_02130"
     CDS             complement(18079..>18523)
                     /locus_tag="PK742_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQU75416.1"
                     /translation="FSAFLAAVFTILNKRLTVSYDPFSITFLELATGFVFLGLLMPVY
                     LQWFPGTPLLPSGADIGYLLILSLVCTTLPFILSLISLRHISAFTANLTINLEPVYGI
                     LLAMLFFHEQDELQPGFYLGAGIILAIVFLHAYIRYRQKKKQLVV"
BASE COUNT         4696 a         4599 c         4321 g         4907 t
ORIGIN      
        1 gaaaagagtt gtgcgcaaac caatacctgt accggcaacg ataggatcgc ggaagaagtt
       61 gacatctacg ctgagcggtg cttgtccgaa agtgacggta ttgtatggat tatcctgttc
      121 aaatggagag aaaccttccc aggcagaacc tgcatcaaag aatccaacaa gttgcaggtt
      181 gcgcagaaac tctgctttaa ctggtttctt gaacagataa gtaaatacgg gcatccgcag
      241 ctctgaattg aatacagcat aagcgttgcc gttgcgtatg ttctgttcat atccccgcac
      301 gggtgttgcc tgtgtctgaa atgcataatt ctgttcctga ttgatctctg tagtgttgtt
      361 gaactgcgga aacaaccagg aatcaacacc tcccaggtag tagatgagtt tctggttacc
      421 aaaagacaca ccaccggcca gcctgttggc ccagatgaac tgcctgtgga tcttctggta
      481 atggcgcaca tcaaaaccaa gtacacccag atagccatcg ttgagactta catccagtcc
      541 gtcgtccaga ttcacggcaa actgtttgtg catttccacc caaactttat accgcatacc
      601 atacatgata ttggtcataa caggcagggt attatcgtgt acatactcca ggcgtaaact
      661 gaaccagtct tccttgtaat tggtctggtt cagactgaat gtatcacttg ccaggaaaat
      721 gaccttatct gcccgatatg ccgccgaagc ccgtaaactg cggatcacat caaaaggata
      781 tttggccgca gcttccacaa tagtggtgcg gggtttggct tctaccggag gaaaccatgc
      841 aggctggaat gaatattgtg cccgctcgtg ctggtaatac cagatcaggc ttttatctat
      901 ccgtttgctc acatcttcat aacgaaggaa atactcacca ccactgaagg aggtaggaaa
      961 acgaagcccg cctgtaaaac gataatcttc aaacagatca ctgatgctta ccgtcataag
     1021 gccgctcaac ggaggctgag aaaagctgcc accggtggca ttgaaattct cataaggatt
     1081 gataagcagg ctgttgtcca gctgtgtttg tacatactca gaactgaatc taagcttgta
     1141 tgcctgaata cgtgattgac gaaatccttc actgaccagg ttattgatat cctttttatc
     1201 agcttcatcc aagacgatga caggttcttc aagcaacact tccggcacat caaatggcgt
     1261 ctgaaaatac ataccctttc gttgttcttc acgcaggctg tcagtagtaa gtgcatctga
     1321 agaaggtttt tcttcttcgc gtaaggaagg gatctctgtt acctcttcca ctaccttttc
     1381 ctgttgcttt cgctcttcat actgtctgta acctgttttc tccagcctgg gcatatcctt
     1441 tccgcctatg gatgccggaa cccgggcaaa atagaactgg ttcctgcctg attcctggaa
     1501 tacctctagc agctttccgg tgcgaagaga aatatcatgt gacaggatat tggtattgta
     1561 attggtgacc ggaaatgtat aggccgtatc tctgaagatc tctacatact tcagagagtc
     1621 aaaatcttct cgtttataga cctcgctgat atcccaggtg gggttgacca ctacactgtc
     1681 tgacagataa agaatggtat tggtgtgatc ataaatagta tccaggtatc cggcaaagcg
     1741 gttcctgata ccattgtaat cactcaggaa ggcaaaatag gtgtcattaa aagcaatggg
     1801 agctgtttca ctgatgtttc tggtatgggt tacctgtacc agatcctttg attttttatc
     1861 gtatttgtaa aaatacagat cattatttcc tacaggcagg atgctgtcaa tgttccgggt
     1921 tctctccagt cgtttgtcat cccggttgga agcaaacaga atgccttcat agccattatc
     1981 tgttttgata taacgcggcg acagatcatc gtaataatcg ttggtgatct gttctaccct
     2041 ggcattattg atgaaatagg tatagatatc actctgtccg ttgttcatca cactcagcac
     2101 cagcatattg ttattctttc cgaacgacat atccagcacc cgctgaaatc gcgaaatgtc
     2161 tttggtctct ttcttcttat cgtcagtatt gtaagtaaac agtacggtct tgtcacgctt
     2221 ttcataaatg gctgccagga tcttgccatc ggggctccat gccaccagcg gataactcag
     2281 atcaatagct ctggttcggg tgcgcatacc accttttttg atcacagcag ctttactttc
     2341 gccggcttcc tgcaaatgga tacggaattt accgatatcg tcagacaggt aagccacatc
     2401 ctgtccgttg gggctgatga ccgggctgtg ataaacaagg tttttcctgg tctttctggc
     2461 catctcttcc gtttcatccg gattgacctt cccgcgtaac tcagcagtgt atctgtcgcg
     2521 gtaatagctg taccattctt ctatggtctg gttgagtgaa gtgcccagaa cgaacaggaa
     2581 accattctcc aggcttcggt tgatacgaat gagatacagc agattcggaa tatcgccggg
     2641 gctgtatttc tcagcgatga aataccagaa agaatgacct gcaaatcgtg catcttcgcc
     2701 agtaagtctg gtaaattgct cataccgacc actcaggatc ccgtcacgga gctggttgtc
     2761 gagatcggca ttccattctt ctccgatata ggacacaagc ccttctacaa accattcggg
     2821 caggttgagc agcacagcgt tctgaacgat ctcctggagg ttgccaccaa agaccatatt
     2881 gttgaccagt atcttgccta caccttccct gatctgggta cgcaggtgct gatggttgcc
     2941 atcaaaatac acaaagagtt tgttgcctat gatcttggtg cgaccgccaa tattgtacag
     3001 cgcttcattg ccaatggcaa aattgctctg attgaaatcg gagatgttgt tgtacacaat
     3061 gatgtctggc ctgctgctca gcttgtaatc aagcaggctt tcgatctcat ccaggtcgtt
     3121 ctcagctatc tgggcggtaa atcttcccag gttctgtccg ccctgattga aatacacgcg
     3181 aaagtgatca ctctcataga attgccaggt aaactctttg tattgtaccc tgttctgacc
     3241 gaacggaatc tgggtgccct gtccggagac aagcaaagga aagaaccctg ccagggccag
     3301 gaatagccag cgccagcgca cgtcagatct gctgttcata catcaaccac taccaaaata
     3361 cgtagctttg gccgaaagtt cagtcaaagc gggaaatttt acaaaataac gaggtttcat
     3421 gagtggtatt gctgtttttc cgttcaatcc ctggcaggaa aatacctatc tgatctacga
     3481 tgcaaacggc atcgctgcgg tcattgatcc gggctgttac agccgggaag aacaacagga
     3541 attgcgcagt ttcatggaag ctcagtcact gcagccactg cacctgctga acacccactg
     3601 ccatatcgat catgtgttgg gaaatgcttt tgtggcagac acctggcaac ttgacctggc
     3661 catgcaccag ggagagatcc cggtgctgaa ggcagtagat acttatgcag acagtatggg
     3721 catccaatac cagcgttcac cggatgccac cctgttgctg aacgaaggtg atgtggtaga
     3781 ggtggggagt attcggctgg aagtcctgtt cacgcccgga cattcgcctg ccagtatctg
     3841 cttctataat cgggccgaag gttatgtgat aggcggcgac gtattgtttc tggacagcat
     3901 tggccgcact gatctgcccg gcggagatat ggatacactg ctggattcca tacgcaataa
     3961 gatcttcccc ttgcccgacg ataccatcgt ctatcccgga cacggacctt ctactactgt
     4021 aggccgggaa aaaaagcaca acccctttct gaacggcagg tatcagccct gatcacctat
     4081 caatccgaag ctgcgcttta cgaatgcaga gagatctgct cctttgagca tgccctgcga
     4141 cagcagagcc aggtcttgta actgcctgac cgcttctttc tgtgctgctt cttctttctt
     4201 cagcaaagca ccaataagcg gatggttggt gttgaccacc aggttgtact gatcgggcat
     4261 ggaagctgca aagtccattc cgttgtagcc gctcatctct ttcattctgc gcatgaattc
     4321 cggccgggta atggcaacag gattatcttc cggagacagc ggtgttagca caacagttgc
     4381 tgcctgctga tcagctgcct gttcaaaaag ctcctgtacc tgtttgcttt cctcttcggt
     4441 caatacagat tctgtggttt catctttgtc tatcagttta tcggctgtat ctgcatctac
     4501 ccgcttgaac tgtacctttt ccagtttgcc ttccaggaag ctgatgaaat gattatcaat
     4561 gatggtatca acctggatga catcgtaacc gcgtgctttc gctgcatcta tatacacatg
     4621 atgttcttcc ggattctgtg agtataacac agtgagtgca tcatgcttat ctgtctgaag
     4681 ctccctgatg tgctccttgt attcctccac cgtgaagtat ttgccatcag tattttgcag
     4741 cagaacgaat ttggaagcct tgtcatagaa tttctcatcg gtaagcatgc catatttcac
     4801 aatgacactg atattttccc attgcttctc aaactcttcc cgttccttgg tgaacatctt
     4861 ttccaggcgt tcagccacct ttttggtgat gtagttattg atctttttga cattgctgtc
     4921 gctttgcaga taggagcggg aaacgttgag cggaatatca ggtgaatcaa tgaccccgtg
     4981 tagtaaagtg agccattcag gcacgatctc tttcacttca tcagtaacaa aaacctgatt
     5041 gcagtacaac tggatcttgt tcttctgcac ctcatagttc tgtttgatct ttgggaagta
     5101 cagcacacct gtgaggttga agggatagtc cacgttcagg tgtatccaga acagtggcgc
     5161 cgaagaatac ggatacagtt cctggtagaa tttccggtaa tcttcatctt ccagttcagc
     5221 aggtttccgg gtccaggcgg gatgggtgtt gttgaccaca ttgggaacct tattcttctt
     5281 ttccttaccc tccacctcta tggtctcttc ctttgtacca aagaccaccg gcaccggcag
     5341 aaaccggcaa tatttgttga gcagctcgtc tatcttttct ttcctgagaa actctttgct
     5401 ctcgccattg atatgcaaaa tgatatcggt gccgcggctt tccttctctg tttcttcgag
     5461 ggtatattcc gggcttccat cacatgacca ttgcactgca ggttcatttt tccaggaccg
     5521 ggttctgatg gtaacatgat ctgccaccat gaaggcactg tagaatccaa gaccgaaatg
     5581 gccaatgatg gtcttaccgt cttctactcc tttgtattta tccagaaatt cctgtgcgct
     5641 ggaaaaagct acctggttga tataacgcct tacttcatcg gcggtcatac caatgccttt
     5701 gtcagaaatg gtcagggtac cttccttctt attcacgata acttctatgc tgatatcgcc
     5761 cagttctcct gtagcttctc ccagactgga taatttcttc agtttctgcg tggcatctac
     5821 tgcattcgaa accagttcac gtaaaaagat ctcatggtca ctgtacagaa acttcttgat
     5881 gatcgggaaa atgttctccg tttgtactgc gatcgttcct ttttcagaca tagccggttt
     5941 tttcatcgcc atgtgcaaag gcagtgccag cagcaaaaaa ctgtcatttt gactgaataa
     6001 gacgcacact ttgtgacagc acctgttttt ttgtactttt tggccagaat tacgcctgat
     6061 ggcccgcacc tcctctgcga gtaagcaaga ccaaaaacaa cgcattgctg atcttcggga
     6121 aaagatcaat gactgctatg taagtaccat aactgacata gagcggtatg ccaaagagat
     6181 cattgaactg gccactgcgg taaaagacag aaatgcagag ggactggcat ttctggatct
     6241 gtctttttac cattgttctt ctaccaatga atacaagaaa tctctggact gtgcccaacg
     6301 tgggatagac ctgctgagcg gtgcccagag aacgtattac ctcccttact atcatttgaa
     6361 ccggggtcgc tgtttccagt tcatgggaga tcagatacgt gcacaggaag aatatctgga
     6421 tgctatttcc atactggaga aaagaaagaa ccggactgtt gaagaaaccc gctggatggg
     6481 ctccagttat tacaatgctt atatcctgtt caaccaggga ggcatggagt tcagccgcga
     6541 agattttctg gaaagagcat atctgctgta tcagcagatc gatgacaaac cgggactcag
     6601 caactgctat aattcttatg ccagcatcca ttacaagaag aaggaattac ctgaaacact
     6661 ggagaacctg gaacaggctt acaagctcag ctctgagatg caggcctata cccatatgag
     6721 cattttttct gccaatattg gtctggtgca tgcccagctg ggtgatgtga gcaaattcag
     6781 agagtatttt gaattgtgtt ccaaacatac ccgaaaaacg aagagtcggt ttcatgaagc
     6841 acatatgaac ggacagatgg gtgaagcact tttctttctg ggtatgtatg atgaatcgct
     6901 ggaaaggctg gctactgcag aagagatctt ttctgaactg ggtgccaaga gtagtctgac
     6961 agagatcctt cgaaaaaggt ctgagatcta tgccttgcag gataattacc gtctggctta
     7021 tgaataccat acccggtatg ctgagcttat acgggagcgc ttcaacgaag aaaaagccac
     7081 cgcaatcatg cgtgcacgga tggtgtacga aatagaagaa aaagaaaatg aagccgccat
     7141 tctgaaggaa aagaacgaag agatccaaca atatatgaac aagttgcagg catccaacaa
     7201 tgaactgcag cagtttgcct atgtggtttc acatgacctt cgcgaacctt tgcgcaccat
     7261 cagcacctat atcaaactgc tggaaaagaa tatgaccggc aggctggaca gagaagaaca
     7321 ggagttcata gattttgtgg tcaatggcac ccgcaatatg aacgaactca tcagtgacct
     7381 gctcactttc agtaagatca gttatatcac tgagaccgaa caggtcgggc tgcagcaact
     7441 ggtacaatct acagccaatg agatcgtgct gcattatctg ccagccaagg atccggtcat
     7501 tcatatcgga aaacttcctg ttattgaggc taacagaaca cacatgaaac agctgtttgg
     7561 caacctgatc tccaatgcca tcaaatacaa caagcacgtt cagccggaga tctccataca
     7621 gtgcgaagac accggtaaaa tgtacaccat cacggtgtct gataacggca taggtattgc
     7681 ccctgaattt cgtgaaaggg tctttcagat ctttcagagg ttacacagca aagcagatta
     7741 ccatggtacc ggtgtaggcc tggccatctg caagaagatc gtagataata tgggtggtca
     7801 gatcacgtta cggggaaaca accgaaaagg ctctgatttc atctttacac ttcctaaatg
     7861 agatacctct tactttgttg ttcgatcctg atggctgcag catgcagtgt gcaatcgccg
     7921 cagtacaaag ggtacagtcc gtttaccata gataaggaag aagcaggccc cgtcatacat
     7981 acaggtgtgc tgttgcacaa tcctaatccc atcggagttt cgttgcgcaa tgcagaactc
     8041 aaagtaactg tcaacagtgt tgaacttggc gatattagtc tggatgagtc agtgcgcatc
     8101 aaaagaaaac aggatttcat gcttccggtc acctttcgca ctacctattc cagcctgtcg
     8161 ggtgccgcac tggccaatct tccggccatc ctgtcaggaa gccctatccc cctgaccgta
     8221 gagggtgagt tacgtgtcag aaaatggctt ttctttcaca agacctttgc ggtggattat
     8281 caggataaaa ttgacctgaa agatatacaa ctttaattta ttcgttccag atatcctgtc
     8341 ggcagtgtga tgatgagcgc ctgccggata gactctatcc gtaccacaaa cctgcgctgg
     8401 ttgcgcactt ccagcaactc gccggtgcat ccagcaagcg ggccgaaggt aacctgtaca
     8461 cgttcgcccg gaataagatc atccggtcct gattctccgg gtaatcctgt ttccacaaaa
     8521 agctgtatgg ttctgatctg atcttccggc acgatggcat tcctgccgcc ggagcgaaca
     8581 aaattcacca ccccttccac catgcgtacg tctgcaaaac aggcaggttc tgtgtgcaca
     8641 aaaaggtatc cggaaaacat gggcacttcc accatctttc tgcggtcact ccactgtcgc
     8701 ataaccttca gcaggggaag aaaacaggta atacctgccc gctccaaacg ctcagccacc
     8761 ttcttctctg atctggaccg ggcataaacc acataccagg acaagccttc cggatagcgc
     8821 atcgtttccg gtctggaaat attcatacag caagtctttc caccctgcca tctttcagca
     8881 catactgctg gccggttccg gaacaaacag catggccact ttcactgaac tccagcttat
     8941 gacccgcttc actcatccag ccggtctgca tggcaggatt gccaatgacc aatgcatatg
     9001 gaagcacatc tttggtaacg acagcaccgg ctcctatgaa agcataggct cctatcgtga
     9061 tgccgcagat gatggtagca ttggcaccaa tggtagcccc tttccttacc agggtagttc
     9121 ggtattcatc ttttctggaa accgctgcac ggggattcag cacattggtg aacaccatgc
     9181 tcgggcccag gaatacatca tcctcacaaa tgacaccggt ataaagcgac acattgttct
     9241 gcaccttcac tccgttaccc aggatcacat ctggcatgac caccacattc tgcccaagtg
     9301 agcagttctt gccaatggtg cagcccggca tcagatgaca aaaatgccag atgctggtgc
     9361 cggctcctat ggtgcaaccc ggatctacca ctgccgtggg atgtatcctg tactttaatt
     9421 cctccataaa cgggtggcag taaaattaag gttttagccg aaggacgtat tttcgcaaca
     9481 tgaaaaagat ccggttgtct ttgctgctgc ctgtaattgc cttgtctgcc tgtcgtacag
     9541 ggtttgacgg caatttgctg gacaaccagg ctcctgatac ccatacggtg gcagatacca
     9601 ttatccgttt tggcgatgac agactggaat ctgaagtggt ggttcgctgg tggggcgacg
     9661 acccggatgg tgttgtggca ggatatgaat ttaccttcgg cgacccatac agtctgatgg
     9721 ccgactggca atttacagaa agacaggaca gcacttttat tctggctccc cctgccggaa
     9781 ctgataccct tgatttttat ttcagcgtgc gggccataga ccaggaaggg ctgcgtgatc
     9841 catcgccagc cacactggca tatccggtta aaaattctcc gccatcagtt gcatttcagc
     9901 cgggactgaa tcagccggtc atcagttttc cggcactgac cttttactgg gaaggcagcg
     9961 atcctgacgg agatgccaac ctgaacggct acgaagtggt gtggaacgat acaaccgtgg
    10021 tacccgttcc gctggaagct ggagcaggtt cactcctgct ggtagccgat gaccccgcca
    10081 tcacatcgat gacctgtaac atgtatgtga atgcatcagc aacgcccctg gcaacctcgc
    10141 tttcaggaat gcaggcacat tcctggaata aactatttat cagggcagta gatcagagtg
    10201 gcgcctattc accctggaca gcttccgatt cagtgtacat caaaccggta caatccaata
    10261 tcctgttggt caatgcctat accactgcca gcaacccaac agatttttat ggcagccacc
    10321 tggtgaacaa tggctttaca gtgctggaca ccattgacct gtttgaaact gcgggtggca
    10381 actacaccca gcaatcggct gacaatctga cccaggaaaa ggtctttgcc atgtttgatt
    10441 gtcttatctg gtacagcaac agtgcagagt cttccttatc acttgggcag cgcaccactt
    10501 ctgaattctt tgacaaaggt ggtcacttgc tcatgagtgt ttatgtgtcg ggatcatttg
    10561 atccgctcag taacttcctg gacttcacgc cggtatccag tttggtcagt cctgctgata
    10621 ccaccctgct gctggaagcc ggcgctttgc ttacacccac tgatgatgct taccccgacc
    10681 tgcaggcaac agccatagtt ggggtggtca aaccggtcaa cctgcagatc ggtgcagcgc
    10741 cagtatatga agcagcgctg acagcaaaag acaatgccac gctgactttt tcgccatgga
    10801 cgggaggtgc ggtggtcatg gccagaaaac cagctgccgg agaagcagat tttatcctgt
    10861 caacactgga acttcagaaa ctggatggcc tgctcaatat ggatgatttt tttaataaga
    10921 tcatgacaga tgcattcgga tactgaacaa aaaaaactgg tggtactggc ttccggaaat
    10981 ggcagcaatg cagagaatct catcagatac tttagcggct caggccttgc aaaagtaacc
    11041 tgtgtagtta ctgaccagcc gggtgcaggt gttattgtaa aagcgggaaa actgggtgtt
    11101 ccggttcgtg tcattacccg ccggatggcc actgacggac caaccatgct gcacttactg
    11161 gaacaggaaa cccccgatct tattttgctt gcaggttacc tgcgtctcat accggaaccg
    11221 gtggttcgca cctatcacga caggatcatc aatatccatc cggccctgct tcccgcctat
    11281 ggcggaaagg gtatgtatgg tgctcatgtg caccaggccg tatttgacaa ccgcgaaaag
    11341 gagaccggta tcaccataca cctggtgaac gaacgatatg atgaaggcag gatcctgttg
    11401 caaagatcta caggcataga acctgatgat accccagact ccatcgcagg taaagtgcat
    11461 gcactggaaa tggaatggta tccaataaca gtgcaggaat acctgtctgc cggctgagcc
    11521 acctgtcagc actgtgtcat tccccgttct gaaaccatcc tcccccttgt ttttacgtat
    11581 attcgtatca taggcaaatt gcctttttat gcgcaccaac tgttttgttc gcctggtatt
    11641 gttgtatatg gccatctgct ggctgttgcc gctgcatgcc cagctccggc tcacagagaa
    11701 caaaggtcaa tggaccgatc agatcaggta caggatggat ctgacctatg gaaacatcta
    11761 tgtggaacag agcggattac tgttcgatat gtctgatccg gaagatctgg agaacatcca
    11821 ctatatcaaa gagaacagca ttccggcaga tccggatgat gtgatcatcc gcaaacacgc
    11881 ctggcgcatg acccctgtcg gtatgcagga gcaacctgct attagcacct cgcaaccatc
    11941 tgctgattac agcaattact ttataggcaa tgatccggac cactgggctt cttctgtaag
    12001 acatttcagc agcatcacca tacatgatgt ttttccgcat acgaatatgc agatcagcag
    12061 ccatgcagac agacctaaat atgaactgac cctgtatccg ggtgcaaacc tgcacaacat
    12121 tgcttttgtc tattccggcc tggattccat gcatctgaaa gatggggcac tggtcatgca
    12181 gtacagcttc gggcaggtga tcgatcagca acctgtggcc tggcagatcg tacacggcaa
    12241 gagggttccc gttccatgcc ggttcatact gaagggagac actgtaggat ttgtttgtcc
    12301 gtccggctac cagacagatc atcctttgat cattgacccg gaaatggttt ttgccagcta
    12361 ttcaggttct acatcagaca actggggata cactgctacc tatgaccaac tgggtcatat
    12421 gtatggcggt ggtattgttt tcggaagtgg ctacccaacc actacgggag cttatgatgt
    12481 gagcttcagc ggtggttcca ttgatatggg cctgaccaag ttcacaccgg atggaagcga
    12541 tctggtctgg agtacttatc tgggtggaag cggtcaggat ctgcctcaca gtatggtggt
    12601 gaacagtgct tttgaactcc tggtatatgg caccaccagc tctcctgatt ttccaatgac
    12661 ccctgatagt tatgatgatg tctatgacga cggacctacc atcagtctgt acagcggctt
    12721 catgaccctt acaggtgccg atatcatcat tgccaaactg agcgatgacg gtaccatgct
    12781 ggaaggatct accttcatgg gaggcacaga cactgacggg ttcaacctgg cttcggccac
    12841 ccagtacaat tatggcgatc attcaagagg tgagatcgtg gtagatgcag ctgataatat
    12901 ttacgtggct tcttccacca gttctgatga tttcccgatc accgccggaa cctttgatga
    12961 tacctacaat ggcagccagg acgccgtggt tttctgtctc aaccccgacc tcagtgacct
    13021 gctgtggagt acctatctgg gaggcacctc cggcgatggt gcctttggcc tgaaggtgag
    13081 cagcagcgga caggtagtag tgaccggagg aacagcctct tcaggttttc ccgttaccgc
    13141 cggcacatgg accacctcct atgccggtgg taccgccgat ggttttgtca gcattctcag
    13201 tgccgatggt tcatcgctcg tagctagtac ctatgccgga accagtcagt atgaccagag
    13261 ctattttgta gaactggatg aatatgataa cgtgtacatc accggacaat caaaaggcag
    13321 ttttcctgtc agcgcaggag tttattcagt taccggtggc aagcagttca tcgtcaaact
    13381 gaacagcagc cttacctctg tgatactgtc aaccgtattc ggttcaggtg gaagttcggt
    13441 caacatctca ccttctgctt ttctggttga tgaatgtgaa aatatctatg tatcaggatg
    13501 gggcggaagc gtcaacaatt ctttcaaccc ggcaaccgga aacaccaccg gactgccaac
    13561 cacggcagat gcacttgatg caactacaga cggatcagat ttctatttct tcgttctgga
    13621 aaaaaacatg gcttctttgc tgtatgccag tttctttggt ggttctcctg cagaacatgt
    13681 ggacggaggc acgagccggt tcgacaaaga aggtaccatc tatcaggcag tctgtgccgg
    13741 ttgtggcggc agcgacggat ttcctaccac tgccggagcc tggagcgaag tgaacggcag
    13801 cttcaattgc aacctggggg tgggcaagat cgcattcaac ctggcaggtg tctatgcaga
    13861 atcagatgcc gagccttcca ttaatggctg cgcacccttt accgtaaatt ttgaaaacct
    13921 gagctcagat gcagaagaat acatctggga ttttggtgat ggcgtgggtg gttctgttga
    13981 attcgaaccc atctatacct acgatagctc tggcacctat acggtcatgc tggtggtcat
    14041 agactcgagc acctgcaata ttgcagacac cagctacctg acagttacgg tatatgaaga
    14101 cagcatcttt gcagatttcg atctgatttc tgaacaggat tgtgaagcac tgacagcttt
    14161 cttctcagat tcttctgaca tactggatac cactacctgg ttctggaatt ttggtgatgg
    14221 cggcacatcc accctgcaaa atccggaaca tacctacacc acaccaggca cctacgaggt
    14281 catgctggtc atctatgatc ccaccagctg caaccagtat gatacagcct atactaccat
    14341 tagttttctt tcggaattca gtgaagacta ctccgtcagc actaccggtt gtttgccact
    14401 ggtagcaaca tttaccagtg agtttaccgg cgccgacagt tacacctgga attttggtga
    14461 tggtacttca ggaaccggcc ctaccattat acacacctat gaagaaacag gcacttacct
    14521 ggtaaccctt tctgttgtgt cttgcggcat tgaagacatc accacttttc cggtcattgt
    14581 ctatggtttt ccggaagcct actttgacag tgaaccggag atcggcctct tgaacacccc
    14641 catcaccttt aacaacctca gcacaggtgc ggtctattat gactggttct tcagtgacgg
    14701 aggatacagc gatgcagaga atgctgtgca caccttcacc tcccttggag gctatgagat
    14761 ctgtctgacc gttaccaacc cggccgactg tgaagacact tattgcagaa gcatcatcat
    14821 tgaaaatgaa ggtgtgatag gggtgccatc cggtttttct cccaataatg acgggtctaa
    14881 tgacgtcctg tacgtacgtg gatttggtat tgagacgatg ctgttccagg tctataaccg
    14941 ttgggggcaa ctggtattct ctaccacaga tcagcacatc ggatgggatg gcacctacca
    15001 gggagtacct caggaagtgg aagtatatat ctatacccta gaagctgaat tcatggatgg
    15061 caagacccat cgggaagaag gtaatatcac cctgatcaga tgaaagggat atggtatatg
    15121 atattaatat ctttcacagg cctgacactg caggcacaag acgtacattt ttctcaattc
    15181 tatgcagctc cgcttctggt aaaccctgca caaacgggca acttcagcgg taccgccagg
    15241 gttggcgcca actaccgtga tcaatgggga agcatcacgg ttccataccg cacctttgac
    15301 ctgtacagtg atgcaggtat ccagccgggg aagagtgtga acagattcgg aatcggacta
    15361 tgtgtattat cagatcaggc cggcgatggc gtgctgcgct ccaacagagc tcagctcagt
    15421 gcagcctatc atgcaatgct gaatgatgat aaaaccgttc gcttgtctgc agggctgagc
    15481 ggtggtgtgg tgcagcgcag tctggacttc agtaagctta catttgacag ccagtggaat
    15541 ggatacaatt tcgattctga tctggcaaat caggaaacag gattcagaga acagttcatc
    15601 tatccggatt tcggaacagg tatattgctc accatgacac cattacctta tgccagatac
    15661 tatgtaggtg catcagccct gcatcttaac cagcctgaag aaagcttttt taccgatggc
    15721 agcaatcagc tgggcattcg gtacctcgtt accggcggcg cttattttcc tgtgtcagag
    15781 caggtaagca tgagcccggc tgcatacctc agcatgcaaa agaaagcact ggaggtgacc
    15841 acaggtacca acttccatat tgaaacagac aatggcagca acagtgccat atttctgctg
    15901 ggtctctggt atcgctattt agacgctgcc tggttcatgg ccggatatgt aacaggaaac
    15961 ctgaccgtct ctgccagtta tgatttcaac ttttcaggat tgaccgctgc atccaacacc
    16021 ctgggaggtt tggaactggc catcatctat actttcaaca agagtcagca caaagatccg
    16081 ctgggttgtc cggcatttga ataacccatc agctgacagc ccggatcagg ttcttggcaa
    16141 tggtgaacgt gaaggtggta cctccaccgg gtgcagaatc cagccagatc tcgcctccat
    16201 gtctttcaat gatctttttg cagagcgcaa gacctatgcc ggtaccactg tattcttcgt
    16261 gggtatgcag tctctggaat atgatgaaga tgcgctgaaa gtattcctga ctgatgccaa
    16321 tgccattgtc tctgacctgg atctcccact tacccggcaa ttcttctact gaaataatga
    16381 tctcaggcgc ttcttcacct ttgaatttga tagcattgtc tatcaggttc tgaaagatct
    16441 gtgtgagctg gatcttattt ccctgaatgg agggcaggtt ctcagccaga atgaccgcat
    16501 ctgtagaatc tatcttttcc ttgagtgccc ggttcaccat ctgcagcacc tcattgagat
    16561 caaccctttc atagtctgta ttgaaacgat tgatccggct gtactccagc aggtctctga
    16621 tcagtgcctg catacgcttg gcaccgtcca caacaaatcc catgaactgc tgaccgtctt
    16681 catcgagcag atggctgtat tttttttcga ggatctgtat gaaactggca actgtgcgca
    16741 ggggttcctg gaggtcatga ctggctacat aggcgaagtg ttcaagctcc tggttgatct
    16801 ccatgagttc cttgttcttt tcctccagct tctgttctgc cttttttctg cggtcaatat
    16861 tctctacaat agcaaccctg accacttcgc cttcagaacg ctccatcagt gatacccagg
    16921 tcttggccca gaaggtggtg ccatcttttc tcacatagcg tttctccaga tcatattcag
    16981 ctatctgacc attgttgagc agttgtacca ttcgcatggt atctggcctg tcttcagaat
    17041 aagtgtaatc aaatacgttg gtacctgtca gaaattccag atcgtactgc agcatttcag
    17101 ccatggcagg attggcatgg aggatctccc cttcattccg ggtgatcatg ataccggatg
    17161 gagaactgtc aaacaccgct ttgaagcgtt tctcagtggc atccagcacc ttcttgttca
    17221 tgcgctcatc ttcaatgtct tccaccagtg ccatgacaag ctgctctttg ggagagatct
    17281 ccaggatggt acaggcaaca cgtgaccagg cagcgtctcc gttagacttt ttcagcctgc
    17341 gttcagccac tgtactttga tgtttgcctt ccagcagatt cgcaaataat gccatggaat
    17401 ccttcaggtc tgcctcaaaa atgagttctt ttaaacgggt tggcggagca tctgcatgat
    17461 aacccattct attaagcagt tgctgattga tattcagtat tgaaccatct gttcgaagca
    17521 ggagaattcc taccgcagtg ttttcaaaca atgcattgaa gcgttgttca ttccgcctca
    17581 tatctgaaaa aagcgacact tgagatgtca catttcttcc tattccgtaa attacttttt
    17641 tatctgaatc tgaaaccagg ctccaggcat agtgcaggca ctctccacct gtagtaagca
    17701 tgcgctgcgt gtattcgatg acctgcagct tcccatcaca catgtcctga aggatctgct
    17761 ccgtttttgg cagatcttcc cgatgtatgg tttgccgggt attctgagcc gcgagttctt
    17821 caagtgcgta tccaaaaacc tgctcccagg cgggattcag cctttttacc tgcttgtcaa
    17881 agccaactac catcagcagg tcatgtgaca agcggaaaaa aacatctgca tctgactgat
    17941 cggaaaggat ttttgttgct tcctgagttt tcttcaccac atcttttgat tcagttttaa
    18001 gcatagcagg ctgtcaccta ttagcttaaa actacttaaa tatgctgcaa aatacaggcg
    18061 aatgcaccat tcttcgggtc acacaaccaa ctgctttttc ttttgcctgt atcggatata
    18121 ggcatgtaaa aaaacaatgg ccaggatgat tcctgcaccc agataaaaac ccggctgtaa
    18181 ttcatcctgc tcgtgaaaga acagcatggc cagcagaatg ccatacacgg gctcgaggtt
    18241 gatggtcaga tttgcagtga atgcgctgat gtgccgcaga ctgatcagtg acaaaataaa
    18301 tggcaaagtg gtgcatacca gagacaatat cagcaggtat cctatatctg cccctgatgg
    18361 cagcaaagga gtacccggaa accactgcag atacacaggc atcagaaggc ccagaaaaac
    18421 aaacccagtt gccagctcca gaaacgtaat agaaaaagga tcatatgaca ccgttagtct
    18481 tttgtttaag atggtgaaaa cagcagccag aaatgctgaa aaa
//
LOCUS       DAOMAX010000018        18208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_320_length_18208_cov_3.726106, whole genome shotgun sequence.
ACCESSION   DAOMAX010000018 DAOMAX010000000
VERSION     DAOMAX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18208
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_18208_cov_3.726106"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..73)
                     /locus_tag="PK742_02135"
     tRNA            complement(2..73)
                     /locus_tag="PK742_02135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Arg,seq:cct)
     gene            410..850
                     /locus_tag="PK742_02140"
     CDS             410..850
                     /locus_tag="PK742_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQU75417.1"
                     /translation="MLSTKCKYAIRSVLLLAAESSLEQKWTIKQISDHLSIPQHFLGK
                     ILQELVPRNIISSMKGPTGGFYMNEQNRSTTLLTLIEVVDGPFFLQGCGLGLEKCSDT
                     NPCPIHDTYKICRNHLRETFSSKKISEFEHDILSKNLFLHGSIK"
     gene            869..1336
                     /locus_tag="PK742_02145"
     CDS             869..1336
                     /locus_tag="PK742_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HQU75418.1"
                     /translation="MKSVLSILLTGCLLAACGPKATDNKTLAEKPNSMFSDDDRSIAE
                     GKGIGEVKEVTLNNPLDASRVQRGLDIYDMKCSACHQLDTVRLVGPGWQGLTQTRKPE
                     WIMNMITNVDVMLEKDPEAQKLLQLCLTRMPNQNVSIGDARDILEFMRQNDGE"
     gene            1363..3339
                     /gene="nosZ"
                     /locus_tag="PK742_02150"
     CDS             1363..3339
                     /gene="nosZ"
                     /locus_tag="PK742_02150"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HQU75419.1"
                     /translation="MKLNMKGLFLAIIMAFVVIAMFFSGCKPKGGISSLTSDAASKVY
                     VPPGSYDEFYLFTSGGFSGQLGVYGLPSGRLLREIPIFSFDSEEGYGFSEETKAMLET
                     SFGFIPWDDSHHPELSQTNGEVDGRWLFINANNTPRIARVDLTTFTTEEIIELPNTGG
                     NHASAFITDNNEYVVSASRFSIPIGQREAAIDSYKEEFDGVATFISVDPKSGTMSPGF
                     QLILPPFDYDLAHTGKGPSSGWMFFTCYNSEEANTLLEVNASQRDKDFIMAINWKKVE
                     EYVKSGNGTKMPINVAHNYRDHNKGGMAVSERYTEITTIKPEEIPGLIYYIPCPKSPH
                     GCDVDPTGQYFCAGGKLAAVVPVYSFEKMQQAIASKSFEGDVEGIPVLKYDAVLQGEV
                     KDAGLGPLHTEFDNEGNAYTSFFVSSEIVKWKVATQEVIDKAPTYYSIGHLCVPGGDS
                     RKPWGKYVIALNKITKDRYLPTGPALTQSAQLYSIEGEKMELLLDFPTIGEPHYAQAL
                     PAELIKDKQVKIFKIEESTHPYVAKGEKEATVYREGNIVHVKMTSIRSHFNPDNIEGI
                     RVGDVVYFHVTNLEQDWDVPHGFAIQGAENAELLIMPGETLTLKWVPKRSGVFPMYCT
                     DFCSALHQEMQGYVRVSPAGSNVPLKFHTNNKGL"
     gene            3394..3987
                     /locus_tag="PK742_02155"
     CDS             3394..3987
                     /locus_tag="PK742_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75420.1"
                     /translation="MKKLNKVTRWMALAGGLIMIPSLLLPIWRIDLFAPQYPEGLYML
                     IWKDHLSGDVQVINGLNHYIGMKHISEDMFPELNYITYVLYGMIGIGIITFSIRRVWM
                     LWTHAVLLISAAGLALYDFYKWGYDYGHNLDPNAAIQVPGMSYQPPLLGHKKLLNFDA
                     WSTPGQGGWFILAGAVLVIGALLLEYFYFKKRSNTLA"
     gene            3984..4421
                     /locus_tag="PK742_02160"
     CDS             3984..4421
                     /locus_tag="PK742_02160"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HQU75421.1"
                     /translation="MKCASIIALCLSIISCQGNKSPRTLTPGTDSCHYCQMTIIDERF
                     TTQLVTEKGKHFIFDDIHCMNAFVQETDNSSLEIAAVYLSDFANPGQYIKPDSAFFLK
                     GDNLRSPMGGNTGTFSNKNSMLETQAILGGESVAFETLFYPSP"
     gene            4424..5674
                     /gene="nosD"
                     /locus_tag="PK742_02165"
     CDS             4424..5674
                     /gene="nosD"
                     /locus_tag="PK742_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HQU75422.1"
                     /translation="MIPIKPTKLTTFLILMLCIGTNAAVHQNEQGHYLSVSVQSMIDE
                     TVPYDTLWINGGYFFENNILITKPITIIGRQNPILDAGGQGQVLIIRSDDVTIEGLTL
                     KGSATSNMTENAGIKIENARRVKIIANTLCDNFFGVYLANSVKCVVRDNIIRGGYERE
                     SNSGNGIHLWQCDSIEITNNVISEHRDAIYLEFVINSHVFGNHCEDNIRYGLHFMFSN
                     NDQYRNNVFNNNGAGVAVMYSSRVEMTDNIFENNLGPSSFGILLKDITDSRITGNQLL
                     KNTIGIRLEGTSRIEIARNNIEDNGWAVLITANCTDNDFENNTFRYNSFDVSTNGKLV
                     LNTFDQNYWDKYEGYDLDRNGMGDVPFHPVSIFATVVSEVPEAIMLYRSFTQYLMDRA
                     EKAIPSVTPENLVDHEPLMKPSVL"
     gene            5671..6387
                     /locus_tag="PK742_02170"
     CDS             5671..6387
                     /locus_tag="PK742_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU75423.1"
                     /translation="MIRIRDLKKSFGKLQVLRGISLEMHAGQVVALIGPNASGKTTLL
                     KCILGMVVPDSGVIEFGGKPVNKEVSYRKHIGYMPQIGRYPENMRVRQVVNMMTDLYP
                     NGADMDTELAEAFYLQEMDQKFMRTLSGGSRQKVSAVLAFLFDVPVLILDEPTAGLDP
                     VSAEVLRQKILREREKGKLIIISSHVLSELEGIATDILYIADGQVKTYQPIAELLKET
                     GTNHTREAIIEIMNRHAGNH"
     gene            6371..7147
                     /locus_tag="PK742_02175"
     CDS             6371..7147
                     /locus_tag="PK742_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQU75424.1"
                     /translation="MQVTIKIARYTLLDLLYNRVTLFYTLFLMGVSLVVFNLDDNVSK
                     GILSLVNIILLIVPLVSIVFATIHMYNSAEFIELLLARPIQRTRILFGHFVGISTALS
                     TAFLIGIGLPVLLYAPSATGITLIAAGWMLTLSFVSIAMLASVSTRDKARGIGIAILL
                     WFLFTMIYDGFVLWILFAFNDYPLEKPVLVLTALNPVDLARIVLLLRIDISAFMGYTG
                     ALYKDYFGTAKGMIITTSVLLCWIIIPMLLAVRRFKSRDI"
     gene            complement(7150..7701)
                     /locus_tag="PK742_02180"
     CDS             complement(7150..7701)
                     /locus_tag="PK742_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQU75425.1"
                     /translation="MKQHIIGLFLPVAILLGSCGTTENGDETSGQGDRFAPGQSAVTD
                     DVSQKNILQIAIASPDHTTLVAGVQAGDLVESLANAGPFTVFAPTNAAFEALPEGTLD
                     DLLKPENKSALRNVLQYHVALSTFTIDNLEDGQILGMANGGNITITKNEDGQVSINGA
                     KVLASVPASNGIVHVIDQVLIPQ"
     gene            complement(7818..9437)
                     /locus_tag="PK742_02185"
     CDS             complement(7818..9437)
                     /locus_tag="PK742_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75426.1"
                     /translation="MRSKLALIFMLVAGGATAQQNNLYLNHQVHQFYEARLQEKGVTV
                     HTSIRPWRMAEIDTLISYDSVFRLQRLPGEKFPGKLVNWIAYDNMVQVNTSDFHLSID
                     PLINFSGGIDLEDNKGLWVNTRGLTVSGDIGEKKKVSFNTTFRENQAKYPTYLTSWIK
                     DYDVVPGQGKVRSFKGTAFDYANSSGYVSYSPNSYFNFQLGHDKNFIGEGYRSMILSD
                     NAFFYPFLKINTSVWRIRYMNLYTQFTDAINGADSIIGVTRKWGSFHYLSFDAWDWAQ
                     IGLFEGIIWPNNDSTLTRGFDPNYLNPIIFFRPVEFGLGSPDNALMGLNLKLKPMSDL
                     IIYGQFLMDDLDVAAARNGEGYYRNKFGMQAGVKYFLHTPDYRHAAVVLAEWNQANPY
                     TYAHKEPVQNYAHLNQPLAHPLGANFREFIIYGSYRLAYRYYASGRFQVAMTGLDSSA
                     ASNVGSNIFLSDFTIPGFPDSYGNFVGQGLETHIFSADLRAGYLVNPKINLNLEGQIF
                     YRTYANDDRAINTLQFMLGLKTDIFNQYYDL"
     gene            complement(9427..10827)
                     /locus_tag="PK742_02190"
     CDS             complement(9427..10827)
                     /locus_tag="PK742_02190"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HQU75427.1"
                     /translation="MVRRYSQFLEITYFLVDLITLNLSFLLGLLITYGRFSKITDRKF
                     AMLLIALNLIWFMVTTIASYYKIDRRSGYEAIVIRFVKVLSIQVLLTFTYIVVMKGYT
                     LSRELLVWTYGCFFVINLIWRTGFETYMKRYRARGGNYRRVIVIGANRTAQQFVNEIT
                     EHNEFGYKFMGYFDDKPQAALQKDTIGGFDDIKWYCFENHIDEIYCALTPAKHEELIN
                     NLMSFSDDNLIRFRIVPEFTEYLSRRFKRVMIEYYGNLPIVTTRPEPLDNLYNRTIKR
                     IFDIVFSSLFLLLIGSWLFPIIALAVKLTSKGPVFFLQKRSGENNSEFLCYKFRTMYV
                     NMEADTKQATKDDPRITRVGRFLRKTNLDEVPQFINVLKGDMSVVGPRPHMLKHTEEY
                     SKIVNSFMVRHFVKPGITGAAQANGYRGDTTDPKLMEKRVQYDVWYLENWSFWLDIKI
                     VFLTVWSMVKGNENAF"
     gene            complement(10942..12177)
                     /locus_tag="PK742_02195"
     CDS             complement(10942..12177)
                     /locus_tag="PK742_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQU75428.1"
                     /translation="MIREVIVLAGGFGTRLASVVQDLPKPMAPVAGKPFLQYILDDLS
                     EQGMQRCILAVGHLRQVIMRYFGDVYAGMELVYSEEEEPLGTGGGIRQAVGYLQGDEA
                     FVINGDTFFGVDLRSLESFHHSHDGSLTLALKKMYRFDRYGTVETDDSGSITAFLEKQ
                     YREEGLINGGVYCLHRSVFTDDLPKVFSFEKEVLEVQFREGHLFGQESEAYFIDIGIP
                     EDYERAQTEMLSRPAFDASWTLFLDRDGVLNTRIPGDYVRNPAQLDILSGVPESLAAL
                     SEIFGRVVVVTNQQGIGKGLYAEDDLALVHEKLMQSVRQAGGRIDAVFFCPHLSGDPM
                     CDCRKPAPGMAMQAKKHFPEIDFSRSVMVGDAASDIRFGRDLGMYTVAIQDFPIILAD
                     ESAPTLSAWATSLKQMKFL"
     gene            complement(12174..12764)
                     /locus_tag="PK742_02200"
     CDS             complement(12174..12764)
                     /locus_tag="PK742_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQU75429.1"
                     /translation="MGSKVEKIRSVISESIRVKQEILNNPELIARCEEIAEKVAEAFR
                     NDKKVLFCGNGGSAADAQHLAAEFSGRFYFDREPLDAEALHVNTSYVTAVGNDYSYDE
                     IFSRVTKAKGRAGDFIFGISTSGNSKNVIRAFEVARERGMIVVAMTGEGGGKMKDHAD
                     YLLNVPSNDTPRIQESHIMLGHIICELVEEALFDRP"
     gene            complement(12749..13777)
                     /locus_tag="PK742_02205"
     CDS             complement(12749..13777)
                     /locus_tag="PK742_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HQU75430.1"
                     /translation="MIIRSKAPLRIGLAGGGTDVSPYSDIYGGCILNVTINKYAYATI
                     IPRKDDKIILHAMDREERIELQATSRLEIDGQLDLLKGVYNRVVRDYNHNAPLSFELN
                     TYVDAPPGSGLGSSSTLVTAIVGAFTEWLKLPLGEYDMAHLAYDIERKDLAFAGGKQD
                     QYAATFGGWNFMEFYKDDKVIVNPLRIRSQYQHELEFNMLMYYTGTSRLSSTIIEAQS
                     SNVQKKSEKSIEAMHNLKEQAYMMKEAILKGDLNRIGDILDYGWNNKKQTASGVSSPV
                     IDEIYEGARKAGATGGKISGAGGGGFMTFYCPGNTRYKVIEVLKSFGGEVHNLEFSKY
                     GLTTWEAK"
     gene            complement(13911..14744)
                     /locus_tag="PK742_02210"
     CDS             complement(13911..14744)
                     /locus_tag="PK742_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HQU75431.1"
                     /translation="MTLARRTLFGLELINDDSFDATIDSMMQFSTQYDAAAGKYPLLF
                     TPNVDDVVKLHEKRYAALAEVLKKSYYILPDGQPVIWASRWLGKPLKRRLPGSELFPI
                     LWKRMVAENRKVMVVAPSEKVGELLQKELPSLVCYVPPFFDENDPAAVLRVVKEARAV
                     MENHRPEFVFLGIRFPKQNYIALGMMGADIPWSEEVQNRPDTQTSAAQVMQQPPLFLL
                     LGASYEFYLDLKKRAPAFWQKIGMEWFYRFTQEPGRLFKRYFIDDIRFFPIVWRERQK
                     H"
     gene            complement(14746..15708)
                     /locus_tag="PK742_02215"
     CDS             complement(14746..15708)
                     /locus_tag="PK742_02215"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQU75432.1"
                     /translation="MKILITGAGGMVGSHMIDHYHAEGEVSITGTFFRPTTDITDIAG
                     KATLKEVDVRYLHGIAQVIMEERPDKIFHLGAQSYPTVSWERPQETMDINVNGTVNVF
                     ESVKLARKQSPDYDPVVVVACSSAQYGASLTPENVPITEDAAFMPLHPYGVSKVAQDL
                     LSYQYFRSDGIRSIRARIFNTTGPRKANDVVADFTHRARLIELGRLQKMRVGNLQTKR
                     AITDVRDLVQALVLLSDKGHWGEAYNISGNKVYTIAEIIPLIEEVIGRGVEIETDPKL
                     LRPTDEPIIYGDSSRLKAHTGWEQQYDLRTTITDMVDYLRTKIL"
     gene            complement(15755..16483)
                     /locus_tag="PK742_02220"
     CDS             complement(15755..16483)
                     /locus_tag="PK742_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQU75433.1"
                     /translation="MVIDHRYQFIFVELPLTATSAISKEIREQYGCEPFLNKHATYDD
                     FLRKATSEEKKYTSIGSVRNPLDQTVSMYFKYKNDLDERFSTGRKRPGKWLRKSLARN
                     RDKVRYQYIIDNQASFEKYFLHFFRWPYSNWSIIHHKKFDHVIRFEHLVEDYRKVFTA
                     VGLPITRDLPQANKTPEKKADFWSYYESDQAKRRAKFVFGPFMRYWGYSFPSSWDHIK
                     EPALAAPLYHLLNMFRKIYWRFLR"
     gene            complement(16527..17366)
                     /locus_tag="PK742_02225"
     CDS             complement(16527..17366)
                     /locus_tag="PK742_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQU75434.1"
                     /translation="MKKILYICLAALGLSACQKDEVTDTKELRYMNLSYGSDPQQTYD
                     MYLPAGRTHETPLMIFIHGGGWAAGDKSDFTAILATLEEQGFAIANINYRLANILTGD
                     IHPAQPNDVRLAVDHLISKAGEYKISADQIVLAGHSAGGHLALYTSLQLNDDDAIKGA
                     ISLAGPTDLTDPYYQSIPDLSLLLLNYIGFTIDAAPGLYTLASPVTHVTAEATPMLLL
                     YGELDVTVPVSNGTSMDAACAAAGADYTYISYPAYGHELGTGTLGLPADVRDPILDFM
                     NNY"
     gene            complement(17363..>18208)
                     /locus_tag="PK742_02230"
     CDS             complement(17363..>18208)
                     /locus_tag="PK742_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU75435.1"
                     /translation="DHYEAMQYVPADFRGKVVFRTHNAEYLIWSRYAEVEPNPIKKIV
                     IRQEARRIKKWELRYVKRADLVLGAPNDNENHEPDPVKRNKKFIEFLHLGQDEQIVLP
                     VPDFTRTELALVYVGTLTWEANIDGLLWLVDGGWEKLKGQFPELKFYIIGKNADQRLM
                     DMAARHRDIILTGFVEDLEDYLPRCRVNVIPLRFGSGMKVKTINGLCRGIPMVSTSIG
                     TEGLRVEDGKDVFIADDLDTFIDRTALLLTDQQRWEELANNSKETAKAHYTWDSLYRI
                     LAENI"
BASE COUNT         4751 a         4420 c         4251 g         4786 t
ORIGIN      
        1 gtagcctcga caggaatcga acctgtatct aaagtttagg aaacttctat tctatccatt
       61 gaactacgag gccgggagcg caaaattacg gaatcaggag gtgctttcaa acgggttgat
      121 tttatgaaat ttcccatgct actgcccgca tgtgtaacag tattgcagga attcgtgcca
      181 tttgacaata gcgcagtcag atatggttat tgcctatagt tcgtgaaaag gcatagtcgc
      241 ctggatttac atacggagta aatggccttc cacttcctgc ttcatcagga aaaggtccca
      301 accatcgtca ccaggctaaa actgacattt atcagcaaac aactggaatg aaagtcatta
      361 atggtattta agacctttta ttcctttatt ctcaaatccg aatacatcca tgctctctac
      421 aaagtgcaaa tatgccatca gatcggtcct tttacttgcc gctgaatcca gcctggaaca
      481 aaagtggacc atcaaacaaa tcagtgacca tctttcgatt cctcaacatt ttctgggtaa
      541 gatacttcag gaactcgttc caagaaatat catctcatct atgaaaggtc cgaccggagg
      601 tttctatatg aatgaacaga accggtcaac tacactactc actctgattg aagtcgtgga
      661 cggaccattc ttccttcagg gatgcgggct gggactggaa aagtgttcag acacgaaccc
      721 ctgtcctatt catgacacct ataagatatg caggaatcac ctgcgtgaaa cattttcaag
      781 taaaaagatc agtgaatttg aacatgatat tttgtccaag aacctgttct tacacgggtc
      841 aatcaaataa aatcacaaac agaaaaaaat gaaatcggta ctatccatcc tgctcaccgg
      901 ctgcctgttg gcggcgtgcg gtcctaaagc cacagacaac aaaacactgg ctgaaaaacc
      961 aaattccatg ttttccgatg atgacagatc catagctgag ggaaaaggta ttggagaggt
     1021 gaaagaggta actctgaaca atccacttga tgcatccagg gtacagaggg gcctggacat
     1081 ttacgatatg aaatgttcgg cctgccatca acttgacacc gtcagactgg ttggtcccgg
     1141 atggcaggga ttaacccaaa cgcggaagcc ggaatggatc atgaatatga tcaccaacgt
     1201 ggatgtcatg ctggagaagg atcctgaagc gcagaaatta ctgcaactgt gtctgaccag
     1261 gatgccgaac cagaatgtat caatcggcga tgccagagat attctggaat tcatgcgaca
     1321 aaacgacgga gaataaaact actcacctat aaatacatcc atatgaaact gaatatgaaa
     1381 gggctgtttc tggctatcat catggcgttt gttgtgatcg ccatgttctt cagcggctgc
     1441 aagcccaaag gcggtatatc cagccttaca tcagatgcag catctaaagt atacgtacca
     1501 ccgggatcct atgacgaatt ctatctgttc acttcaggtg gtttcagcgg acaactcgga
     1561 gtatacggtc ttccatccgg acgtttgctt cgggagatac ccatcttctc ctttgactcg
     1621 gaggaaggat acggcttcag cgaggaaacc aaggccatgc tggaaacatc ctttggattc
     1681 ataccctggg atgattcaca tcaccctgag ctttcgcaga ccaatggcga agtggatgga
     1741 agatggttat ttatcaatgc gaacaatacg ccaaggatcg caagggtgga ccttaccaca
     1801 ttcactacag aagagatcat agaactcccc aatacaggtg gtaaccatgc ttctgctttc
     1861 atcactgata acaatgaata tgtggtaagt gcctccagat tctctatccc gataggacaa
     1921 cgcgaagctg ccatagacag ctacaaagaa gagttcgacg gcgtggccac attcattagt
     1981 gtagatccaa aaagcggtac gatgagcccg ggattccagc tcattcttcc cccgtttgac
     2041 tatgatctgg cccatacagg aaaaggacca tccagcggtt ggatgttttt tacctgttac
     2101 aacagcgagg aagccaatac gctgctggaa gtaaatgcca gtcaacgtga taaagacttt
     2161 atcatggcca tcaactggaa aaaagttgaa gagtatgtca aatccggaaa tggaaccaaa
     2221 atgccaatta atgtggcaca caattatcgt gatcacaaca aggggggcat ggcggtatcc
     2281 gagcgttata ctgagatcac cacaatcaaa cctgaggaga tacccggatt gatctattac
     2341 atcccatgtc cgaagtctcc acacggttgt gatgtggatc ccacgggcca gtatttctgt
     2401 gcaggtggca agctggcagc cgttgtacca gtatactcat ttgaaaaaat gcagcaagct
     2461 atagcttcaa aatcctttga aggtgatgtg gaagggatcc cggttctgaa gtatgatgcc
     2521 gtattacaag gtgaagtgaa agatgccgga ctcggaccgc ttcacacaga atttgataac
     2581 gaaggaaacg cttacacatc cttcttcgtg tcttctgaga tcgtcaaatg gaaagtggct
     2641 acgcaggaag tgatagataa agcacctacc tactattcca tcgggcacct ctgcgttcct
     2701 ggaggcgaca gcagaaaacc ctggggtaaa tatgtgatcg ccctgaataa gataaccaaa
     2761 gaccgatacc tgcctacagg accggcattg acacaaagtg cccagctgta cagcatcgag
     2821 ggtgagaaaa tggaactgct gctagacttc cctactatcg gcgaaccgca ttatgcacag
     2881 gcattaccag ctgaacttat caaagacaag caggtcaaga tctttaaaat tgaggaatcg
     2941 acacacccgt atgtggcaaa aggagaaaaa gaagccacag tatatcgtga aggaaatatt
     3001 gtacatgtga aaatgacctc catcagatca catttcaacc ctgataatat tgaaggtatc
     3061 agagttggtg atgtggtata cttccatgtt accaacctgg aacaggactg ggacgttcct
     3121 cacggatttg ccatccaggg tgctgagaac gcagaattac tgatcatgcc cggtgagaca
     3181 ctcaccctta aatgggtccc taaacgatct ggtgtatttc caatgtattg tactgacttc
     3241 tgctcagcac tgcatcagga aatgcagggt tatgtcagag tatctccggc aggcagcaat
     3301 gttccgttga agttccatac caataacaaa ggattgtgag taaactgaac ataaggggga
     3361 gcgcaggctc ccccttatga taaagaaaag tgcatgaaaa aactgaacaa ggtaacccga
     3421 tggatggcac tggcgggagg gctgatcatg ataccttcat tgctccttcc catatggcgg
     3481 atagacctct ttgctcccca gtacccggaa gggctttaca tgctgatctg gaaggatcac
     3541 ctgtctggcg atgtacaggt catcaatgga ttaaaccatt acattggcat gaaacatatc
     3601 agtgaagaca tgtttcccga attgaactac attacctatg tattgtatgg aatgatcgga
     3661 attgggatca tcaccttttc aataagaaga gtctggatgt tgtggacaca tgccgtgctg
     3721 cttatttctg cagccgggct ggccttgtat gacttttaca aatggggtta cgattatggt
     3781 cataatctgg atccaaacgc agccatacaa gttcccggta tgagttacca acccccgttg
     3841 cttggccata agaaactact caacttcgat gcctggagca ctccggggca gggcggatgg
     3901 ttcattttgg ctggtgccgt tctggtcatt ggtgctttat tgctggaata tttctacttc
     3961 aaaaaaaggt ctaatacact tgcatgaaat gtgcgtcgat catagccttg tgcttatcta
     4021 tcatcagctg tcagggaaat aagagcccca gaacccttac tcccggaact gacagttgtc
     4081 attattgtca gatgacgata atagatgagc gtttcaccac acaactggtc acagaaaagg
     4141 ggaaacattt catattcgat gacatacatt gcatgaacgc atttgtacag gaaaccgata
     4201 acagctctct tgaaatagct gctgtatatt tatctgactt tgctaacccg ggtcagtata
     4261 tcaaaccaga ttcagcattt tttctcaagg gtgacaattt gcggtctccc atgggaggaa
     4321 ataccggaac tttctcaaac aaaaactcga tgttggaaac ccaggcgatt ctgggaggtg
     4381 aatctgttgc atttgaaaca ctgttttacc ccagtccctg atcatgatcc cgattaagcc
     4441 aaccaaactg accactttcc tgattctgat gctttgtatt ggaacgaatg ctgctgtgca
     4501 tcagaatgaa caaggtcatt atctgtctgt atcggtccag tccatgattg atgaaacagt
     4561 gccatacgac acgttatgga tcaatggcgg ttactttttt gagaacaata ttctgatcac
     4621 caaacccatt acaatcattg gtcgccaaaa tccgattctg gatgccggag gacaaggaca
     4681 agtgctgatc atcaggtcag atgatgtaac tattgaaggc cttaccctta aaggaagtgc
     4741 aacttcaaat atgacagaga atgcaggcat aaagatcgag aatgccaggc gggtaaaaat
     4801 aattgccaat accctttgtg ataatttttt tggcgtgtac ctggctaatt ctgtaaagtg
     4861 tgtggtcaga gataatatca tccggggtgg atacgaaaga gaatctaact ccggtaatgg
     4921 cattcatttg tggcaatgcg acagtattga aatcactaac aatgttatca gtgaacatcg
     4981 cgatgccatc tatctggaat ttgtgatcaa cagccatgta ttcggtaatc attgtgagga
     5041 caatatcagg tatggattac actttatgtt ttccaacaat gatcagtacc ggaacaatgt
     5101 ctttaataat aatggtgcgg gtgttgcggt catgtacagt tccagagttg aaatgacaga
     5161 caatatcttt gaaaacaatc ttggtccgtc atcttttggt attctgctca aggatattac
     5221 agacagccgt ataacgggca atcaattgct aaagaacact attggtatac gactggaagg
     5281 aaccagtcga attgagatag cacgaaataa catagaagat aacggatggg cagttcttat
     5341 tactgccaat tgcacggata atgattttga aaacaacacc tttcgataca attcattcga
     5401 tgtatcgacc aatggcaaac ttgtgctgaa tacatttgat cagaactatt gggacaaata
     5461 cgaaggatat gacctggata gaaacggcat gggcgacgtt ccttttcacc ccgtcagtat
     5521 ttttgccaca gttgtaagtg aagtacccga ggctatcatg ttgtacagaa gttttaccca
     5581 atatctgatg gacagggcag aaaaagccat acccagtgtt acacctgaaa atctggttga
     5641 tcacgagcca ctaatgaaac cttctgtgct atgatcagaa tcagagatct taagaaatca
     5701 tttggaaaac ttcaggtact tcgtggtatt tcactggaga tgcatgcggg gcaggtcgtg
     5761 gcactgatcg ggccaaatgc atccgggaaa accacattat tgaaatgtat tctaggtatg
     5821 gtagtgccgg acagtggtgt catcgagttc ggtgggaagc ctgtcaataa ggaagtgagc
     5881 taccgaaagc atatcggtta tatgccacag atcggcaggt atcctgaaaa tatgcgagtc
     5941 agacaggtgg taaatatgat gacggacctg tatccgaatg gcgctgacat ggataccgag
     6001 ctcgcagaag cattttacct gcaggaaatg gatcagaaat tcatgcgtac cctgtcaggt
     6061 ggatccagac aaaaggtcag tgctgtactg gcattcttgt ttgatgtacc tgtgctcatt
     6121 ctggacgaac caaccgctgg cctggatcct gtatctgcag aagtgctcag gcaaaaaata
     6181 cttcgtgaaa gggaaaaagg taagctcatc atcatttcgt cacatgtgct cagcgaactg
     6241 gaaggtattg cgacagacat cttatatata gcagacgggc aggttaaaac atatcagccg
     6301 atcgccgaac ttctgaaaga gaccggtacc aatcataccc gtgaagcaat cattgaaatc
     6361 atgaacaggc atgcaggtaa ccattaaaat agcgagatac acattgctgg atctcctgta
     6421 taacagggtg accctttttt ataccctttt cctgatgggt gtatccctgg tggtgttcaa
     6481 cctggacgac aatgtctcta aaggcattct gagcctcgtg aatatcatcc tgcttattgt
     6541 acctcttgtc agcatcgttt ttgccactat tcacatgtat aattctgctg agttcattga
     6601 gttgctgctt gccagaccaa ttcagcgaac ccgcatacta ttcggacatt ttgttggtat
     6661 ttccactgcc ctttccaccg catttctcat cggcatcggg ctaccggttc tgttatatgc
     6721 accatcagca actggtatta cactcattgc ggcaggctgg atgcttactt taagctttgt
     6781 ttcgatcgct atgctcgctt ccgtatccac ccgcgacaaa gcccgcggaa taggcatcgc
     6841 cattcttctt tggttcctgt ttaccatgat ctatgatgga tttgtactct ggatcctgtt
     6901 tgccttcaac gactatcctt tagaaaaacc agtacttgtg ctcactgccc tgaacccggt
     6961 tgacctggct cgcatagttc tgttgctcag gatcgatatc tctgccttta tgggatatac
     7021 cggtgctttg tacaaggatt actttggcac agcaaaaggg atgatcatca caacgagcgt
     7081 tttgctctgc tggatcatca tccctatgct cctggctgta agaagattca agagcagaga
     7141 tatctgatat cactgcggga tcaacacctg atcgatcacg tgaacgatac cattggaggc
     7201 cggtacagat gccagtactt tggcgccgtt gatacttacc tgtccgtctt cattttttgt
     7261 aatagtgata ttacctccat tcgccatacc gaggatctga ccatcttcca gattatcaat
     7321 ggtaaaagtg cttagtgcca catggtattg caatacattt cgcaatgcac tcttgttctc
     7381 tggtttcagc aggtcatcca gcgtaccttc tggtaaggcc tcaaatgctg cattagttgg
     7441 cgcaaacact gtgaacggac cagcattggc cagtgattcg acaagatctc cggcctgaac
     7501 gcctgccaca agtgtggtat gatccggaga tgcaatcgct atctgaagga tgttcttttg
     7561 agaaacatcg tccgtaactg cagattgtcc tggtgcaaat ctgtcaccct ggccagaagt
     7621 ttcgtcgcca ttctctgtgg taccgcatga ccccaacagg atcgctacag gcaggaataa
     7681 accgataata tgttgtttca tagctgttat tttcagctaa actgaccttt ttatcttaaa
     7741 tatttattga tgttagtcag cctgagacag ttgacaatcg tgactgcaaa atggtgactt
     7801 aggtatcctt atatacccta cagatcgtaa tactgattga aaatatcggt cttcaggccc
     7861 agcatgaact gcagcgtatt gatggcccgg tcatcatttg cgtaggtcct gtaaaagatc
     7921 tgtccttcca ggttcagatt gatctttggg ttgaccagat atcctgctct gaggtctgca
     7981 ctgaaaatat gcgtttccaa accctgacct acaaaattgc cataagaatc cgggaaaccc
     8041 gggatagtaa aatcagataa aaagatattc gaccccacat tggatgcagc tgaagagtcg
     8101 agtcctgtca tagcgacctg gaatcttccg ctggcatagt atctgtaagc cagccgatag
     8161 cttccgtata taatgaactc acggaagttg gctcccagcg ggtgcgccag tggctggttc
     8221 aggtgcgcat aattctgcac gggctccttg tgcgcatagg tgtagggatt ggcctggttc
     8281 cattctgcca gcaccacagc tgcatgccgg taatcaggtg tatgcagaaa atatttcacc
     8341 cctgcctgca tgccgaattt gttccggtaa tagccctctc cgttccgtgc ggcagccaca
     8401 tccaggtcat ccatcagaaa ctgaccatat atgatcagat cactcatggg tttgagtttc
     8461 agattcaacc ccatcagtgc attatcaggt gatccaagac caaattccac cggccggaaa
     8521 aagatgatcg ggttcaggta attcggatcg aagcccctgg tgagggtgga gtcattgttg
     8581 ggccagatga taccttcgaa cagcccgatc tgcgcccagt cccaggcatc aaaactcaga
     8641 taatgaaaag atccccattt gcgggtcacc ccaatgatgc tgtctgctcc gttgatcgca
     8701 tccgtgaact gggtgtacag gttcatatac ctgatacgcc agactgaggt attgatcttg
     8761 aggaacggat aaaagaaggc attgtcactc aggatcatgg atcggtatcc ttcgccaatg
     8821 aaattcttgt cgtgccccag ctgaaagtta aaatagctat tgggtgagta actcacatat
     8881 ccgctgctgt tggcatagtc aaaggcagtt cctttgaaac tacgcacttt accctggccg
     8941 ggaaccacat cataatcctt gatccagctg gtcagatagg ttggatactt ggcctggttt
     9001 tcacgaaagg tggtattgaa cgagaccttc ttcttttcgc cgatatcgcc agacaccgta
     9061 agtcccctgg tattgaccca gagccccttg ttatcctcca gatcgatgcc tcctgaaaaa
     9121 ttaatgagcg ggtcaatact caggtgaaaa tcactggtgt tgacctgaac catgttatca
     9181 tatgcgatcc agttgaccag tttaccgggg aacttttctc cgggcagccg ctgcaaccga
     9241 aaaacactgt catagctgat gagggtatcg atctctgcca tacgccaggg acggatggaa
     9301 gtatgcaccg tcaccccttt ttcctgtagc ctggcttcat agaactgatg tacctggtgg
     9361 ttcagataca ggttattctg ctgtgcggta gcacctcctg caaccagcat gaagatcaaa
     9421 gccagtttag aacgcattct cattgccttt gaccatgctc cataccgtca ggaaaacgat
     9481 cttgatgtcg agccagaaac tccagttctc cagataccat acatcgtact gtacccgttt
     9541 ttccatgagt ttcggatcgg tggtatcacc cctgtatccg ttggcctgag cggctccggt
     9601 aatgccgggt ttcacaaagt gacgcaccat gaaactgttg acgatcttgg agtactcttc
     9661 ggtatgcttg agcatgtgtg gtcttggacc aaccacagac atatctccct tcagcacgtt
     9721 gatgaactga ggaacctcat ccagatttgt cttacgcagg aagcggccca ctctggtgat
     9781 cctgggatca tctttggttg cctgcttcgt atctgcctcc atgttgacat acatggtccg
     9841 gaacttataa cacagaaact cagaattatt ttcgccgctt cgcttttgca gaaagaacac
     9901 agggccttta gaggtcagtt tcactgcaag tgcaatgatc gggaagagcc aggaaccgat
     9961 cagcaacagg aagaggctgc tgaaaacaat atcaaaaatc cgcttgatgg ttctgttata
    10021 caggttatcc agtggctccg gccgggtggt cacgatgggc agattgccat aatactcgat
    10081 catgacacgt ttgaaccttc ggctgaggta ttctgtaaac tccggtacga tccggaatcg
    10141 gataagatta tcatcagaga aactcatcag gttgttgatc agttcttcat gtttggcagg
    10201 ggtcagtgca caatagatct catcaatgtg attctcgaaa caataccatt tgatatcatc
    10261 aaatccacca atggtatcct tctgcaaggc agcctggggc ttatcatcaa aataccccat
    10321 gaacttgtaa ccaaactcat tgtgttctgt aatctcattc acaaactgct gggcagtgcg
    10381 gttggctcca attactataa ccctgcgata gttgccccct ctggcacggt accgcttcat
    10441 ataggtctca aagcctgtac gccagatcag gttgatgaca aaaaaacagc cgtaggtcca
    10501 gaccagcaat tctcgtgaca gggtgtaccc cttcatgacc actatatagg tgaaggtgag
    10561 cagaacctgg atagaaagga ctttgacaaa gcggataaca atggcttcgt aaccgctgcg
    10621 gcgatcaatt ttatagtagc ttgcaatggt agttaccatg aaccagatca ggttcagggc
    10681 gatgagaagc atagcaaatt tcctgtcagt gatcttagag aagcgcccgt acgtaatgag
    10741 cagccccagc aagaatgaca ggttgagcgt tatcagatcg accagaaaat aggtgatctc
    10801 caggaactgg ctgtatcttc tgaccatgcg gttgttgtca atggtagatg aaaaatgccc
    10861 gcctcaggtt gcaataagtc cgggcttaat catctgaatt gccctgcaaa attacatttt
    10921 cagtgctgta atacagataa cttacagaaa tttcatctgt ttaagagagg ttgcccaggc
    10981 agaaagggtt ggtgcacttt catcggcaag aattatcgga aaatcctgaa tagcaacggt
    11041 gtacattccc agatcacgcc caaaacgaat atcagaagct gcatctccta ccatgacact
    11101 tcgcgaaaaa tcgatctcag ggaaatgctt tttagcctgc atagccatgc ctggagccgg
    11161 tttgcggcag tcgcacatag gatcgccgga aaggtgcgga caaaaaaata cagcatcaat
    11221 tcggccacct gcctgtctga cggactgcat cagtttttcg tgcaccagtg cgaggtcgtc
    11281 ttcagcatac agccccttac ctattccctg ctgattggtg accaccacca cgcggccaaa
    11341 gatctctgac aaagctgcca gcgattccgg tacacccgac aaaatatcca gctgagccgg
    11401 gtttctgaca taatcgccgg gaattctggt attcagcacc ccatcccggt ccaggaaaag
    11461 ggtccaggag gcatcgaaag caggacgaga cagcatctct gtttgcgccc gttcatagtc
    11521 ctcaggaata ccgatatcaa tgaaataagc ttcactttcc tgtccgaaca ggtgcccttc
    11581 tcggaattgt acctccagca cttccttttc aaatgagaat accttcggaa ggtcatctgt
    11641 aaataccgat ctgtgcaggc aatacacccc accgttgatc aggccttctt cgcggtattg
    11701 tttttccaga aaagcagtga tgcttcctga atcatctgtt tctactgtac cgtaccggtc
    11761 aaacctgtac attttcttca gcgccagtgt aagtgatccg tcgtgagaat gatgaaagga
    11821 ttccagtgac cgcagatcca caccaaaaaa agtatcgcca ttgatgacaa aagcttcatc
    11881 accctgcaga tacccaaccg cctgtctgat tcctccgcct gttcccaatg gttcttcctc
    11941 ttcgctgtac accagttcca tgccggcata aacatctccg aaataccgca tgatgacctg
    12001 acgaaggtga cccaccgcca ggatacaacg ctgcatgcct tgttcagaga ggtcgtccag
    12061 aatgtactgg agaaaaggct tacccgccac cggtgccata ggcttgggca gatcctgcac
    12121 tacagaagcc agtctggtac caaatccacc ggccagaacg atgacctcac ggatcatggg
    12181 cgatcgaaga gagcttcttc taccagttcg cagatgatgt gacccagcat gatgtggctt
    12241 tcctgaattc tgggagtatc gttgctggga acattgagga gataatcggc gtgatctttc
    12301 atctttccgc cgccctcgcc cgtcatagcc actacgatca tgcctcgttc gcgggctact
    12361 tcaaaagcac gaataacgtt ctttgaattg cctgaggtgg agatgccaaa aatgaaatcg
    12421 ccggcacggc ctttggcttt ggttacacgg ctgaagatct catcataaga atagtcatta
    12481 cccactgcgg taacataaga ggtatttaca tgcagtgcct ctgcatcaag cggttcgcgg
    12541 tcaaaataga accggccgct gaattctgct gccaggtgct gcgcatcggc ggcagaacca
    12601 ccattgccac agaacaatac tttcttatcg ttgcggaatg cttccgccac tttctctgcg
    12661 atctcttcgc atcttgcaat caactccggg ttgttgagga tctcttgctt gaccctgata
    12721 ctttcggaaa tgaccgaacg aattttttct actttgcttc ccatgtggtt aatccgtatt
    12781 tactgaattc cagattgtgt acttcaccgc caaatgactt tagcacttca atgaccttgt
    12841 aacgggtgtt tcccggacaa taaaaggtca tgaaaccgcc accaccagca ccggatatct
    12901 ttccgccggt agctcctgct tttctggcac cttcgtatat ctcatcaatg acagggctgc
    12961 tcactcctga ggcagtctgt ttcttgttgt tccagccata gtccaggata tcgccaatgc
    13021 ggttcaggtc gcctttcagg atggcttcct tcatcatgta ggcctgttcc ttcaggttgt
    13081 gcatggcctc aatacttttc tccgacttct tctgcacatt ggaagactgc gcctcgatga
    13141 tggtagaaga caagcggctg gtaccggtgt agtacatcag catgttgaac tcgagttcat
    13201 gctgatactg tgaccggatg cgcagcgggt tcacaatgac cttgtcatct ttatagaatt
    13261 ccatgaaatt ccatcccccg aaggtagctg catactggtc ctgctttcca ccggcaaaag
    13321 ccagatcttt ccgctcgata tcataagcca gatgggccat atcgtattcg ccaagcggca
    13381 gtttcagcca ttctgtaaag gcacccacga tggccgtgac cagcgttgac gatgacccca
    13441 gtccggagcc cggaggtgca tccacatagg tattgagctc gaatgacaga ggtgcgttgt
    13501 ggttatagtc gcgaaccaca cggttataca cccctttcag aagatccagt tgcccatcga
    13561 tctccagcct ggaggtagcc tgcagttcta tgcgttcttc gcgatccata gcatgcagaa
    13621 tgatcttgtc atccttgcgt ggaatgatgg ttgcgtatgc atacttattg atggtcacgt
    13681 tcaggataca gccaccgtag atgtcgctgt agggactcac gtctgttcct cctcctgcca
    13741 gtccgatgcg caatggcgct ttgcttctga tgatcatatt gccctgttag gagctgcaaa
    13801 gttgctgaaa aatgggctta tttacgcctt ttacagatta acttgttgct aaatgcacct
    13861 ttccttcatg cgatagactg agaattgagc cctgccgttg caccaacctg tcagtgcttt
    13921 tgtctttcac gccagactat tggaaagaaa cggatatcat ctatgaaata ccttttgaac
    13981 aggcgacccg gttcctgggt gaaacggtag aaccattcca ttcctatctt ttgccagaat
    14041 gcaggagccc gttttttcag gtcaaggtag aattcatagg aagcccctaa caggaggaat
    14101 agcggaggtt gctgcatgac ctgtgctgcg gatgtttgtg tatcaggtct gttctgtacc
    14161 tcttctgacc agggaatatc cgctcccatc atgcccagtg cgatgtagtt ctgtttgggg
    14221 aagcgaatgc ccagaaagac aaattccggc cgatggtttt ccataacagc ccgggcttct
    14281 ttgaccacac gaagtactgc cgccggatca ttttcatcaa agaagggcgg cacataacaa
    14341 acgagtgacg gcaattcctt ctgcaagagt tcgcccactt tctcagaagg agccactacc
    14401 atgacctttc tgttctcagc caccatgcgt ttccaaagga tcgggaagag ctcactgccg
    14461 ggaagtcttc tttttaaggg ttttcccagc caccgggaag cccagatcac aggctgacca
    14521 tcgggcagga tatagtagct ttttttcaat acttcagcca gtgctgcata cctcttctcg
    14581 tgcagtttga ccacatcatc cacattcggt gtgaacagca gtggatactt tccagctgca
    14641 gcgtcatact gagtactgaa ctgcatcatg gaatcaatgg tcgcatcaaa actgtcatcg
    14701 ttgatcagct ccagtccgaa gagggtcctg cgtgccagtg tcatcttaca ggattttggt
    14761 gcgcaggtag tccaccatat cagtgatggt ggtgcgcaga tcatattgtt gttcccaacc
    14821 ggtatgggct ttcagtctgg aactgtcgcc ataaatgatc ggttcatccg tgggtcgcag
    14881 cagtttcggg tcggtttcaa tttccactcc cctgcctatc acctcttcga taagcggaat
    14941 gatctctgct atggtataga ccttgttgcc gctgatgttg tatgcttcgc cccagtgtcc
    15001 cttatcactg agcaacacca gtgcctgcac gaggtcacgc acatctgtga tggcccgttt
    15061 tgtctgcagg ttacccaccc gcatcttctg cagtctgccc agttcaatga gcctggcccg
    15121 gtgggtgaaa tcggccacta catcattggc ttttctgggt cctgtagtat tgaagatcct
    15181 ggcccggata cttctgatcc cgtcgcttct gaaatactga taggacagca gatcctgcgc
    15241 caccttgctg acaccatacg ggtgcagcgg cataaaagcg gcgtcttctg tgatgggtac
    15301 attttccggt gtcagtgatg cgccatattg tgcactgctg caggccacca ccacaacagg
    15361 atcatagtcg ggagattgtt ttctggccag tttgacagac tcgaatacat tgacggtgcc
    15421 gttcacattg atgtccatgg tctcctgcgg tctttcccag gaaacggtgg gatagctctg
    15481 ggcgcccagg tggaagatct tgtcaggccg ctcttccatg atgacctgcg caatgccatg
    15541 caggtagcgc acatccactt ctttcagcgt ggccttgccg gctatgtctg tgatatctgt
    15601 ggtcggacga aaaaaagtac cggtaatgct gacttcgccc tctgcatgat aatggtctat
    15661 catgtgagaa cctaccatac ctccggcacc tgtgatgagt atcttcatat actgcagtta
    15721 cgctgcaaat atcggaaaga aaaaacgagg cagattagcg aaggaatctc cagtagatct
    15781 tcctgaacat attgagcagg tgatacagcg gagcggccag tgcgggttct ttgatatgat
    15841 cccaggacga aggaaaggaa tagccccaat aacgcatgaa cggaccgaac acgaatttcg
    15901 ccctgcgctt tgcctgatca ctttcataat aactccagaa atcagctttc ttttccggag
    15961 ttttgttggc ctgcggaagg tctctggtaa tgggaagtcc cacggcagtg aatactttcc
    16021 ggtaatcctc caccaggtgt tcaaagcgga tcacatgatc gaatttcttg tgatggatga
    16081 tgctccagtt gctgtagggc catcggaaga aatgaaggaa gtacttttcg aaacttgcct
    16141 ggttgtctat gatgtactgg tacctgactt tgtctctgtt gcgggcaagt gatttccgca
    16201 gccattttcc cggtcgcttt cttccggtgg agaatctttc gtcgaggtcg ttcttgtact
    16261 tgaagtacat gctcacggtc tgatcgagcg ggttgcgcac agaaccgatg ctggtgtatt
    16321 tcttttcttc tgaagtagcc ttccgcagaa aatcatcgta ggtggcatgc ttgttcaaaa
    16381 aaggctcaca accgtactgc tctctgattt cttttgaaat ggcagaggta gcagtaaggg
    16441 gcagttctac aaaaatgaat tgatagcgat ggtctattac catagcgcaa aggtactttg
    16501 cctgctgcga ttacaccacc tgcagcctaa tagttgttca tgaaatccag gatcggatcg
    16561 cggacatctg ccggcagacc cagcgttcct gttcccagct catgtccgta ggcgggataa
    16621 gagatgtagg tgtagtcagc tccggcagcg gcgcaggcgg catccataga agtaccattg
    16681 gaaacaggta cagtaacatc cagctcgccg tagagcaaga gcatgggcgt agcctcagcg
    16741 gtcacatggg taacaggact tgccagggta tataaacctg gtgctgcatc tatggtgaac
    16801 ccgatataat tcagcagcag cagactcaga tcagggatgc tctggtaata gggatcggtc
    16861 agatccgttg gaccggccag tgaaatggcc ccttttatgg catcatcatc attcagctgc
    16921 agactggtgt ataaggccag gtgtccaccc gcactgtgac ctgccagaac gatctgatct
    16981 gcagagatct tgtactctcc tgctttgctg atcagatggt ccaccgccag ccgcacatca
    17041 ttgggctgag ccggatggat atcacctgtc aggatattgg ccagtctgta gttgatattg
    17101 gcaatggcaa agccctgttc ttccagggta gccaggatgg cagtaaaatc agacttatcg
    17161 cctgccgccc atcccccgcc atgaatgaag atcatcagcg gcgtttcatg tgttcttcct
    17221 gcaggcaggt acatatcgta ggtttgctgc ggatccgatc cgtaagacag attcatatat
    17281 cgcagctcct tggtatctgt cacttcgtcc ttctgacaag cagaaagccc cagagctgcc
    17341 aggcatatat agagtatctt tttcatatgt tttctgccag tatccggtag aggctgtccc
    17401 aggtatagtg cgcctttgcc gtctccttac tgttgttggc taactcctcc cagcgctgct
    17461 gatctgtcag caataacgcg gttctgtcaa taaaggtatc cagatcatcg gcaatgaata
    17521 catcttttcc atcttccacc cggaggcctt ctgtgccaat gctcgtactg accatcggaa
    17581 tgccccggca aaggccgttg atggtcttca ctttcatgcc gctgccaaag cgaagcggga
    17641 tcacattgac ccggcaacgc ggcaggtagt cttccaggtc ttccacaaag ccggtcagga
    17701 tgatatcacg atgtctggct gccatgtcca tcagtctctg atcagcattc ttaccgatga
    17761 tgtagaactt cagctcagga aactgtccct tcagtttttc ccagccgccg tccaccagcc
    17821 acagcaggcc gtcaatattg gcttcccagg taagtgttcc tacatataca agggccagct
    17881 ccgttcgggt gaaatcaggt accggcagta cgatctgttc gtcctgaccg aggtgcagaa
    17941 actcgatgaa tttcttgttc cgtttcaccg gatcaggctc gtggttctca ttatcattgg
    18001 gtgcaccaag cacaagatcg gcacgtttga cgtagcgcag ttcccatttt ttaatgcgtc
    18061 tggcttcctg acgtatcact atttttttaa tgggattcgg ttccacttct gcatatctgc
    18121 tccagatcag gtattctgca ttgtgggtac ggaacaccac cttgcccctg aaatctgccg
    18181 gcacatactg catggcctca tagtgatc
//
LOCUS       DAOMAX010000019        18147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_323_length_18147_cov_4.206721, whole genome shotgun sequence.
ACCESSION   DAOMAX010000019 DAOMAX010000000
VERSION     DAOMAX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18147
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_18147_cov_4.206721"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK742_02235"
     CDS             <1..472
                     /locus_tag="PK742_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQU75436.1"
                     /translation="EFLETKGIHRYMIEIGGEIRARGLNSRNTGWTVGIRKPEELSAQ
                     LVSTITLNDLSLATSGNYNKYVVVDGRKMGHTIDPHTGRPAVNELLSATLITRNCMHA
                     DALATVCMVGGFEQAKELILRQESLEGYLIYSDEAGKMATWISPGMEELIREVQ"
     gene            complement(473..667)
                     /locus_tag="PK742_02240"
     CDS             complement(473..667)
                     /locus_tag="PK742_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75437.1"
                     /translation="MDSLIYAIILIGAAFFLFAIRIIFVKGGEFHGTCATNNPMLKNE
                     VGECTMCGRKAGEPCGNPES"
     gene            733..1521
                     /gene="mazG"
                     /locus_tag="PK742_02245"
     CDS             733..1521
                     /gene="mazG"
                     /locus_tag="PK742_02245"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQU75438.1"
                     /translation="MKSRQEKMEAFGRLLDIMDDLRTQCPWDKKQTIRSLRNLTIEET
                     YELADAISEEDWTGIGEELGDLLLHMIFYSRIGEEQGKFDVADVLHKQCEKLIHRHPH
                     IYGDLTVADEEEVKRNWEQLKLQEGKKSVLQGVPGSLPALIKAYRIQDKAGQVNFQWE
                     ETAQVWDKVAEEMAELKEQTDHATPDQEKIEEEFGDLMFALVNLSRYLKVDPENALER
                     TNKKFIRRFSYIEEHAAAAGKALKDMTLTEMDALWNEAKLAEHP"
     gene            complement(1568..4486)
                     /locus_tag="PK742_02250"
     CDS             complement(1568..4486)
                     /locus_tag="PK742_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU75439.1"
                     /translation="MNRNLYLTLCLLLTGLTAFGQNRTISGTVKDAETNEALPGVSVF
                     VQEDPSTGTITDLDGQYSLAVPESSTTLVFKFVGYEDQYVAIAGQNKIDVSLELTSLN
                     LDAVVISASRRQEKILDAPASISLITTKQINNTVAISPTDNLKGVPGVDIINTGLVQT
                     NVVTRGFNNIFSGALLTMVDNRYAAVPSLRVNVNMFIPTDNSDLERIEVLRGPASALY
                     GPNCANGVLHMITKSPLDQDDRFQTTVSLGGGFRQYISDTIAIANPIDPITGLVDTAY
                     SPLFDDDAFGDRAMYSAAIRHSGKINDKLGYKILGTYFAGNDWLYDDPFEPNYIYKGY
                     QTSEGRVTTDTVPTLNTRNNDINKMGVDLRLDYRPDNISEWILAGGITQNDGIEMTGI
                     GAGQAIDWRYYYGQIRYRRDRLFTQVFLNGSDAGQTYLFRTGDQIIDKSKQLAAQVQY
                     SSDHMNNDLTLIYGIDFIETMPQTESTINGQYENDDNITEVGAYIQGKYMINSKLELL
                     AALRGDYHNFVDQPFVSPRAAVVYKPSTRQTFRLTYNRAFSAPSSNNLNLDILQLADL
                     GDLGAFGQSLFGLDYIPGIGVRATGNRGGFTYSYDNNGFAQFLSPYASFIGDASNTYY
                     SISGNDQLNNITWDMATVLLFQGFAEATGLPLAQVQLLFGPLLPDDISSVSNVMRLLN
                     LTTEEFDLIDPNNIQDFGGILNSATNTFELGWKGSLMDDKVFATVDVYQTTIQDFVGP
                     LTNITPNVFLDPESLTSGIYAEMFAAWEDPSNAVAVSLLTGALDENGDGDAFPEWVAT
                     VVGAGAGVPMGTVVPTELDQPAIYVTYVNLGDVTVYGADLGATIYVNDDFRVSLGYSW
                     IDKDSIALEGAQLGYVALNAPANKASLKLSYDIKKIDLNVGATFRWQASYPANSGAYV
                     GTVESINDLDLTLNYAPHYMDRTVFSLLVSNVYNREQQYFVGAPVMGRSLFFKATRTF
                     "
     gene            4566..8267
                     /locus_tag="PK742_02255"
     CDS             4566..8267
                     /locus_tag="PK742_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75440.1"
                     /translation="MRSNTRLFHSLLLLTWLVSIFWLVRGLVFSRQLPDVEQEARKVV
                     NSYRALEDKILQVVTDRSVANRLLTDSYTFDEFESLCRLPFGLVVLQGDSAAFWTSNA
                     IMPVEAQFQANDAPLFITEKNGSYVLYQHTFEDSLHTIAFLPLRMDFGINNRYLSDIE
                     VEGIHIPVYFDLSPVKQDRSLPVTSVTGEEVFFLVPDVSRMGQEGSHPLLIIPFLLFF
                     LTTLYVLYQLRRPLLNHARVRWPVLAYLLLLVLSYAVLHFGLFSRMEGTSLFDPVIYA
                     SSWMATSLGDLLVRSLLLLLFVLALSQQTLGNRKALLITAHVMLALLVWFTVLMINSL
                     VIDSAIAFDIRELFLLNSNTLVAAVCIATTCLATALLAAKLLPVRSGNRQWWWIFPVT
                     VLFILLLQGLINGMPDRHFGGFSLVLLVLMGVFGFRQKRSTSLLFNLIWLLAFSMLSA
                     WIMNDALYRKSEENKRLYAYKKSVEKDPLAEYLFSGQREEISHLLKVQLSQQPPQQVT
                     IRQGLVNQLVQDMQSSYFKKYQTEIYFFLDDSFLVGTGQLDHPSIYDFINDIENNGSY
                     TTSPDLYFINDYSGNFFYIAELYIPYDSGHELQVYFRLIPRRFNSESLYPELLVDDDL
                     RLPSGQERFNYAVYYDNNLVERKGAYSYPVMDVFYLKKGEEYADKIINGYDHFIYAAG
                     ESKKVVVSEPDQDMLTAVSYFSFLFFFFLLFFGLLSGMELLERMANGEVTLKGLLDTS
                     LKNKIQLAIVSLVVFTFLSLGIATIIYITGQFDRSNGDKLLEKIEAVQTNIDYLITDN
                     RRNSDRILQQQVVVRRLGNRIAELSSIHDMDINLYSKNGLLINSSQPDIFEKGLISPK
                     MNPIAFFKMTCEKETWFIHSEQIGRLTFLSAYVPILDESGEVLFYVNLPYFATEKNLQ
                     AEISSLMVALVNVYVLLLVLSALIALLVSRNITLPLSLVAGTFRSVQLGKKNEPVSWP
                     HKDEIGLLVTEYNKMLEQLEQSARLLAQSERESAWRDMAKQVAHEIKNPLTPMRLSIQ
                     HLQRAIESGRPDVEELTSRVAKTLMEQIDNLSFIASEFSNFAVMPKAVNEPIELGRLL
                     SNVSSLFDETERTRITLELPEAEMIIWADRNQILRVFNNLVKNGLQAIPEDREGSMII
                     RLSASQGRALVTVQDNGAGIPDDIKDQVFVPNFTTKSSGMGIGLALSKNIVESAGGYI
                     WFESEPGSGTTFFVEFPLYDGRQEEGADT"
     gene            8291..9292
                     /locus_tag="PK742_02260"
     CDS             8291..9292
                     /locus_tag="PK742_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQU75441.1"
                     /translation="MKLFSGWRTGIFFTVVYLYIIGSFVWWWWLLQSNNKENYETLIQ
                     RERITYMSYGRSEEGFYDTPFFLELERDYHRKTTMLIGEGIVFISLISLGFVRLRNTF
                     REELALTRQQNNFLLSITHELKSPLASLKLSMQTMQKHDLERDKVRRLADISMNDVDR
                     LEALVENILLASKMDSSDFSISRDPFLLSDVARQIFDRTADKHATERIFSADIAPEIY
                     LLGDRLIFSSVIYNLLENAIKYSRQGDRVELLLRQTKDHIVLEVRDTGIGIADEEKPR
                     VFQRFYRVGREETRTTKGTGLGLFIVKQAVHFHQGTISVSDNLPQGTRFIIHIPVEK"
     gene            9397..10098
                     /locus_tag="PK742_02265"
     CDS             9397..10098
                     /locus_tag="PK742_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU75442.1"
                     /translation="MQYRILLVEDEDHIRENLKLNLELEDYEVVAVDNGSRAIKDFRE
                     QRFDLVILDVMMPEMDGFDVCEQIRLEDLNTPILFLTAKDTSQDKIQGLKTGADDYMT
                     KPFNLEELLLRVRNLIRRSAKTPAARKEFVEYTFGGNTINFMTYEAQGVDGRHKLTNK
                     ELKLLKLLIDRKGEVVSRQHILQTVWGYDVFPSTRTIDNFILAFRKYFEQDPKNPTYF
                     HSIRGVGYKFTDAEA"
     gene            complement(10165..10599)
                     /locus_tag="PK742_02270"
     CDS             complement(10165..10599)
                     /locus_tag="PK742_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQU75443.1"
                     /translation="MALIKRKGIEFPFGPTLLSDFFSGDRFFGKDVFTPERMPAVNIE
                     ENDTQFVIEMAVPGMRREDFSVGVENGLITISAERKNEEVEEKRNFTRREFSFESFSR
                     SFTLPENVTEDNIEAVYLDGLLKLTIPKKESKEVHTKNIEIR"
     gene            complement(10730..13213)
                     /locus_tag="PK742_02275"
     CDS             complement(10730..13213)
                     /locus_tag="PK742_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HQU75444.1"
                     /translation="MIRSITLWCLSGLLTFASSVSVLAQQPPYAGGSPASVVGMVLEK
                     QSGTPVEFAAVSILAESDSSLRSGTITDEKGMFRIEGLSNGGYFLKVEFLGYDPYFTN
                     FVLSSDAPFYKAGKIELSVSSTVLEGVEISAERSYFQQSIDKKVYNIDKDIVASSGTA
                     SEALQNIPSITVDIDGNVSLRGNSNVRIFIDGKPSGLMGSSMVAILEQIPASSIESIE
                     VVTNPSARYEAEGGGGIINIVLKKNKKVGLNGVATVGGSTFPGYDGSLTLNYRDKKWN
                     LFGSYSYDNRTRDGWSEVSRKVFYEDNDSTSYYNSDGESTDNRSGHNIRGGADYYIND
                     RNTIGFSALYSTGNNNNDDLNNYFFYDNDSLLSQYNQRQGDGSSDNHNYNIGLNYRKT
                     WENPKHLWTTDAFFSNGFGDGYTGYTEFTYDNSGAEDLAARINQQVLSDDRNQDININ
                     SDYIHPFKNGNQLEAGARFTREFRDNDILSESFDGESGVFANDDSISNRFRFDQQVLA
                     AYTIWNSSIGKLGYQLGLRAEQTFLTSELVTTGQSFNKDYFRLFPSVHLKYEIKKGVE
                     TNASYSRRINRPRSFFLNPFPEFSDPYTYRQGNPFLLPEDEHSFELGLTRIWDKHTLS
                     SSVFYKHTDGEFSAYTTVDSNGLSRTTFENFSTEDSYGAEFVVRNEFFPWWSATSSLN
                     VNRTILDASNIEAGLSNALTNYNARVMMFFKVLKQTSLQATYSYWRPWVMAQGQPRPF
                     TFIDLGIRSDFFSNKLSVNLTVSDVFDTREFYVTNEGFNFSQEFRRKRESQAVSIRLS
                     YKFGQQDNNRRRGGQGDWDNNGGGGMDMF"
     gene            13333..14874
                     /locus_tag="PK742_02280"
     CDS             13333..14874
                     /locus_tag="PK742_02280"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HQU75445.1"
                     /translation="MEDKREKLLRFRQEALAGGGEKRIASQHKKGKLTARERIQLLMD
                     PDSFEEIGQLKVHRCHDFGMENERYYGDGVVTGYGTVNGRLIYVYAQDFTVFGGSLSE
                     THAEKICRIMDLAMQNGAPVVGLNDSGGARIQEGVNSLGGYADLFYRNTLASGVIPQI
                     SAIMGPCAGGAVYSPAITDFILMTEGNSYMFVTGPNVVKTVTHEDVSFEELGGASTHS
                     TKSGVAHFTGENGVMVIEQLKKLLSYIPQNCEEEAPMTAYQPADESRPKLGAIIPENP
                     NHPYDIREVVDEVVDSNSFFEVHKQYAANIVVGFARLGGRSIGVVANQPAVLAGVLDI
                     EASRKAARFVRFCDCFNIPLLVFEDVPGFLPGTDQEWNGIITNGAKLLYAFCEATVPR
                     VTVITRKAYGGAYDVMNSKHIGADMNFAWPTAEIAVMGPKGAAEIIFKKEIEEADDKE
                     AKLQEKIDEYTRKFADPYRAAFRGFVDEVILPENTREKLIRAFDMLRNKAVKLPRKKH
                     GNIPL"
     gene            14883..15971
                     /locus_tag="PK742_02285"
     CDS             14883..15971
                     /locus_tag="PK742_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HQU75446.1"
                     /translation="MAVRKTTIRQEDLNFLEQYLNNPSPTGNEAGGQKIWLEYMKPYI
                     DDHYVDNYGSVAAIINPGKEFRVVIEAHADEIAWAVHLITEDGFIYVERIGGSDHMIA
                     PSKRVNIHTDKGVVKGVFGWPAIHTRHYGKEESPELKNIFVDCGCSSKKEVEKLGVQV
                     GDLITFDDDFMVLNKNMYVGRALDNRIGGFMIAQVARLLKENKNKLPFTLYVVNSVQE
                     EIGLRGAEMMAHYIKPHVAIVTDVTHDSNTPMISKNLEGDIRAGRGPSLTKGPAVHNK
                     LLKLVMDTAAEKKIPFQRDVASRSTGTDTDAFAYSNGGVPSCLISLPLRYMHTTVESA
                     HKDDVDAVIRLIYEVLLKLNPKFNFKYL"
     gene            complement(15972..17141)
                     /locus_tag="PK742_02290"
     CDS             complement(15972..17141)
                     /locus_tag="PK742_02290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQU75447.1"
                     /translation="MKKQTLFILIAAIWLMTAGITSCTNNGGKTFTVEGSIGGLENAT
                     LLLETMTFPNMNGTPAFTAIDTVTADPDGNFIFSGHLRERSICRIKMENNPRYYLLLN
                     IYDEKLHISGSTNDPKPLFEGSTATLVLNGFIDELRNKNMEIIDFSNNVLSQSAVLGD
                     SLTAVMNDRKDAMIDSFYAYVANFADTTKELTNKIVALENLLYDRQFEIIKEKAAPII
                     ATGDTSNVYVRELTSKVNRYQQIMDEEAANSFLGKTATDLNLPDPDGKPIQLSSLRGK
                     YVLLDFWASWCAPCRKENPNLVALYEQYKDKGFTVYSVSLDDNRDAWMQAIRSDKLSW
                     PSHVSQLQKWNSSAAAAYNVTGIPASFLLDTNGVVIAENIRSEQLSQILAGLLGE"
     gene            complement(17138..>18147)
                     /gene="gatB"
                     /locus_tag="PK742_02295"
     CDS             complement(17138..>18147)
                     /gene="gatB"
                     /locus_tag="PK742_02295"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQU75448.1"
                     /translation="NRAGTPLIEIVSEPDLRSGEEAYEYLTEIRKLVRYLAICDGNME
                     EGSLRCDANISVRKKGASEFGVKVEVKNMNSIRNVKRAIDHEIERQIAAIENGETISQ
                     ETRSFDAAAGTTFSMRSKEAAHDYRYFPEPDLPPLRIHAAMVEEIRSGMPALPKELQK
                     RYEQELGLSTYDAALLTAEKSVVTYFEALIQHTTRYKAAANWILGPVREWLNAGARDM
                     DDFPVAPAQLAALLALIDEGKLSFSAAAQQLLPVWVEEPDTAPDALAQKLNLIQETDE
                     AFIEQLVDEALKAFPDKVAEYRNGKKGLIGLFMGEVMKRSKGKADPKVATALLQKKLE
                     A"
BASE COUNT         4724 a         4113 c         4484 g         4826 t
ORIGIN      
        1 agagtttctg gaaacaaagg gcattcacag atatatgatc gaaataggcg gtgagatcag
       61 ggccaggggt ttgaacagtc ggaatacagg ctggacggtc ggtatccgca agccggaaga
      121 actgtcggca cagctggtaa gcaccatcac cctgaatgat ctgtcgctgg ctaccagtgg
      181 aaattacaat aagtatgtag tggttgatgg ccggaagatg gggcatacga ttgatccgca
      241 taccggcagg cctgcagtca atgaattgct cagtgccaca ttgatcaccc ggaactgtat
      301 gcatgcagat gccctggcaa ctgtttgtat ggtaggtgga tttgaacagg caaaggagct
      361 cattctcaga caggaaagcc tggaaggata cctgatatat tctgacgaag ctggaaaaat
      421 ggcaacatgg atatcgcccg gaatggaaga gctgatcagg gaggtgcagt gatcaggatt
      481 caggattgcc acatggctct cctgcttttc gcccgcacat ggtgcattcg cctacttcat
      541 tcttcagcat ggggttgttg gtggcacagg ttccgtgaaa ttcgccgcct ttgacaaaga
      601 tgatacgaat agcgaaaagg aaaaatgcgg cacctatcag aatgatggca tagatcagag
      661 aatccatcga cgaaaatttt tacaaattta ctgcaattac tatctaaaca ggcagataac
      721 gtcaatagtt caatgaaaag cagacaggaa aaaatggaag ccttcggcag gctgttagac
      781 atcatggatg acctgaggac ccaatgcccc tgggataaga aacagaccat tcgttctctc
      841 cgcaacctga ccatcgaaga gacctatgag cttgcagatg ccatttcaga agaagactgg
      901 acagggatcg gtgaagagtt gggagatctg ctattgcaca tgatcttcta cagcaggatc
      961 ggagaggaac agggaaagtt cgatgtggca gatgtgctgc ataaacagtg cgaaaagctg
     1021 atccacaggc acccgcacat ttatggagat cttacggtgg ccgatgaaga agaagtgaaa
     1081 cgcaactggg aacaactgaa actgcaggaa ggaaaaaagt cagtgctgca gggagtgcca
     1141 ggttctttac ccgcactcat aaaagcttat cgtattcagg acaaagcagg gcaggtaaac
     1201 tttcagtggg aggagaccgc tcaggtgtgg gataaggtag ctgaagaaat ggctgaactg
     1261 aaagaacaga ccgatcatgc tacacctgat caggaaaaga tagaagaaga gtttggagat
     1321 ctgatgtttg ccctggtgaa tctgtcacgc tacctcaagg tagatccgga aaatgcactg
     1381 gagcgcacaa acaagaaatt catacgtcgt ttcagctata ttgaagagca tgctgctgct
     1441 gccggtaagg ccctgaagga tatgaccctg acagaaatgg acgccttgtg gaatgaagca
     1501 aaattagctg aacatccata aaaaaagccc gccgggatag cgggctttta ctaagtagtg
     1561 tgtgtgttca gaatgttcgg gtggccttga aaaacaggga tcttcccata acgggtgctc
     1621 ccacaaaata ctgttgctct ctgttgtaaa cattggaaac taacaggctg aaaacagttc
     1681 tgtccatata gtgtggtgca tagttcagtg tcagatccag gtcgttgatg gattcaacgg
     1741 ttccaacata agcaccagag ttggccggat aagaagcctg ccagcggaaa gtagctccca
     1801 cattcaggtc tatctttttg atatcataac tcagcttgag gctggccttg ttggcgggtg
     1861 catttaatgc cacatagccc agctgagcac cttccagagc gatgctgtct ttatctatcc
     1921 aggaatatcc cagagatacc cggaagtcat cattgacata aatggtggca cccagatcag
     1981 caccgtaaac ggtcacatcg cccaggttca cataggtcac ataaatagca ggctgatcca
     2041 gttcagttgg cacaacagta cccattggaa caccggcacc tgcgcctacc acagtggcaa
     2101 cccattcagg gaacgcatct ccatcgccat tctcatccag tgcaccggta agcaggctca
     2161 ccgctactgc attggaaggg tcttcccacg cagcaaacat ttctgcatag atacctgaag
     2221 taagtgattc aggatccagg aatacgttgg gcgtgatatt ggttaacggg cccacaaaat
     2281 cctggatggt ggtctgatat acatcaaccg ttgcaaagac tttgtcatcc atcaaactgc
     2341 ctttccagcc cagttcaaat gtattggtgg cactgttcag gatgccgccg aaatcctgaa
     2401 tattattcgg gtctatcagg tcaaattctt ctgtggtcag attcagtaaa cgcatcacat
     2461 tggatacact gctgatatca tccggcaaca gcgggccgaa taagagctgc acctgagcca
     2521 agggcaagcc tgttgcttca gcaaatccct gaaacagcaa tacggtggcc atgtcccagg
     2581 tgatattatt caactgatca tttccggaga tggaataata ggtgttggaa gcatcgccaa
     2641 tgaaggaagc gtatggcgag aggaactggg cgaatccatt gttatcataa gagtaggtga
     2701 atccgccgcg gtttccggta gcacgtacac ctattcccgg aatgtagtcc agtccgaaaa
     2761 gtgattgtcc gaatgctccc agatcaccca ggtcagccag ttgcaggata tccaggttca
     2821 ggttattgct gctcggggca ctgaacgctc tgttgtaggt cagtctgaat gtctggcgtg
     2881 tgctgggctt gtacaccact gcagctcttg gtgatacaaa tggctgatca acaaaattat
     2941 ggtaatcgcc gcgaagcgct gcaagcagtt caagcttgct gttgatcata tactttcctt
     3001 gtatgtaggc gcctacttct gtaatgttgt catcgttctc atactggccg ttgatggtac
     3061 tttccgtttg cggcatggtc tcaatgaagt caattccgta gatcagggtc aggtcattat
     3121 tcatgtgatc tgagctgtat tgtacctgcg ctgccagttg cttggatttg tcaatgatct
     3181 gatcgccggt gcggaagaga taggtctgtc ctgcatcact gccattcaga aatacctggg
     3241 tgaacaagcg atcacggcgg tatctgatct gcccgtagta atacctccag tcaatggcct
     3301 gtcctgcacc gataccggtc atttcaatac catcattctg agtaatacct cctgccagta
     3361 tccattcaga aatgttatcc ggacggtaat ccaggcgcag gtcaaccccc attttattga
     3421 tgtcattgtt tctggtattc aatgtaggta ctgtatctgt ggttacacgt ccttcgctgg
     3481 tctggtatcc tttgtagata tagttcggtt caaatggatc atcatacaac cagtcgtttc
     3541 ctgcaaaata ggtacccagt atcttatatc ccagtttgtc attgatcttc ccgctgtggc
     3601 ggatggctgc tgaatacatg gcacggtcgc cgaaggcatc atcatcgaac aagggagaat
     3661 aggcggtatc taccaggcct gtgatggggt ctatcgggtt ggcgatggca atggtatcgc
     3721 tgatgtattg cctgaatcct ccgcccaggc tgacagtagt ctggaagcga tcatcctgat
     3781 caagcggaga tttggtgatc atatgcaaca caccgttggc acagttgggt ccgtaaagtg
     3841 cagatgccgg gccgcgtaaa acttcaatac gctccaggtc actgttgtct gtagggataa
     3901 acatatttac gttcacacgc aaagaaggta ctgctgcata acggttatca accatcgtca
     3961 gcaatgcgcc gctgaatata ttattgaagc cacgggtaac cacgttggtc tgcaccagac
     4021 cggtgttgat gatatctaca ccgggaacgc ccttcaggtt gtcagtcggg ctgatggcca
     4081 cggtattgtt gatctgtttc gttgtgatca ggctgatact ggcaggtgcg tccaggatct
     4141 tttcctgcct gcgggaagct gatataacca ctgcatccag gttcagagag gtcagctcca
     4201 gtgagacatc aattttgttt tggccggcaa tagccacgta ctgatcttca tatcctacaa
     4261 atttgaagac cagtgtggta ctgctttcag gcacagccag gctgtattga ccatccaggt
     4321 ctgttatggt tccggtggaa gggtcttcct gcacaaaaac actgacaccc ggaagtgcct
     4381 catttgtttc ggcatctttg actgttccgc tgatggtgcg gttctgacca aaggcagtca
     4441 gcccggtcag tagtaaacat aaggtcaggt acaggttgcg gttcatagat ttggattgaa
     4501 atttgatgct aataataaag tttatttttg attttttaac acagccggcc ctccctactg
     4561 atagcatgcg atctaataca cgcttatttc attcattact tctgcttaca tggcttgtca
     4621 gcattttctg gctggtgagg gggctggtgt tctccaggca gttgccggat gtggaacagg
     4681 aagcccgaaa agtggtgaac agttaccggg cgctggaaga caagatactt caggtggtaa
     4741 ctgaccggtc tgttgccaac cgcctgttga cagactctta tacgtttgat gagtttgaat
     4801 ctctgtgcag gttgccattt ggtctggtgg tgttacaggg tgattctgcg gccttctgga
     4861 ccagcaacgc catcatgccg gtggaagcac agtttcaggc aaatgatgct cctctgttca
     4921 ttactgaaaa aaatggcagt tatgtattgt atcagcacac tttcgaagac agcctgcata
     4981 ccattgcatt tctgccctta cgcatggact ttggtatcaa caaccggtat ctgtcagata
     5041 tagaggtgga aggtattcat atacctgttt actttgatct cagtccggta aagcaggata
     5101 gatcgttgcc ggtcacatct gttaccggcg aagaggtgtt tttcctggta cctgatgttt
     5161 ccaggatggg gcaggaagga tcgcatccct tgctgatcat tcctttcctg ctgttcttcc
     5221 tgacaacact gtatgtattg taccagctgc ggcgcccact tttaaatcat gcccgtgttc
     5281 gctggcctgt acttgcttat ttgttgttgc tggtattgtc ctatgccgta cttcattttg
     5341 gcctgttcag cagaatggaa ggtacctcac tgttcgatcc ggttatttat gcatcatcct
     5401 ggatggccac ttctttaggt gatctgcttg tgcgttccct gttattgtta ttgtttgtgc
     5461 ttgcactttc acaacaaaca ctgggcaata gaaaggcatt gttgatcacg gcacatgtca
     5521 tgcttgcatt gctggtctgg tttacagttc tgatgatcaa cagcctggta atagacagtg
     5581 ccattgcttt tgatatcaga gagttatttc ttcttaatag caatacactt gttgcggctg
     5641 tttgtattgc caccacctgt ctggccactg cattacttgc ggcaaagctg ctgccggtgc
     5701 gctccggaaa ccgccaatgg tggtggatat ttcctgttac agtgctcttt atcctgttgt
     5761 tacaagggct gattaatgga atgcctgaca ggcattttgg tggtttctca cttgttcttt
     5821 tggttctgat gggtgtattc ggattccggc aaaagcgcag tacttcattg ctgttcaatc
     5881 tgatctggct actggcattt tccatgctta gtgcgtggat catgaatgat gccctgtacc
     5941 ggaaatctga agagaacaaa agattgtatg cctataagaa atcagtagaa aaggatccgc
     6001 ttgcagagta tctgttcagt ggtcagcgag aagagatcag ccacctcctg aaagtacagc
     6061 tgtctcagca gccacctcag caggtcacca tcaggcaggg attggtcaac cagctggtgc
     6121 aggacatgca atccagctat ttcaagaagt atcagacaga gatctatttt tttctggatg
     6181 attcttttct ggtaggtacc gggcaacttg atcatccaag tatatatgat ttcatcaatg
     6241 acattgagaa caacggctct tataccactt cacctgatct gtattttatc aatgactaca
     6301 gcgggaattt cttttatata gcagagctct atattccata tgattccggg catgagctgc
     6361 aggtatattt caggctgatt ccccggcgct ttaacagcga aagtctgtat cctgaattgc
     6421 tggtagatga tgaccttcgc ctgccgtccg gacaggagcg ttttaattat gccgtgtatt
     6481 atgataacaa tctggtagag cgcaagggag catattccta tccggtaatg gatgtgttct
     6541 atctgaagaa aggagaggaa tatgcagata agatcatcaa tggctatgat cattttattt
     6601 atgcagcagg tgaaagcaag aaggtagtgg tttctgaacc tgatcaggat atgcttactg
     6661 cagtatctta tttcagcttc ctgttctttt tctttttgtt gttctttggc cttttgtcag
     6721 gtatggaact tctggaacgg atggcaaacg gtgaggtcac gctgaaaggt ttgctggata
     6781 ccagtctgaa aaacaagata cagcttgcca ttgtcagttt ggtggtattc acctttctgt
     6841 cgctgggaat agcaaccatc atatacatta ccggacaatt tgatcgaagt aacggagata
     6901 aactgctgga aaagatcgaa gcagtacaga ccaatattga ctatctgatc acagacaacc
     6961 ggcgcaacag cgacaggatc ctgcaacaac aggtggtggt gcggcgtctc ggtaatcgca
     7021 tagcagagct gtcgtccata catgatatgg acatcaacct gtacagcaaa aatggactgc
     7081 ttatcaattc atcacagcct gatatatttg aaaaaggact tatttctcca aaaatgaatc
     7141 ctatagcctt tttcaagatg acctgcgaga aggaaacctg gttcatacat tcagagcaga
     7201 tcggaaggtt gacttttttg tctgcatatg taccaatact ggatgagtct ggtgaggtgc
     7261 tgttctatgt taatctgcca tattttgcaa ctgagaaaaa cctgcaggca gaaatttcca
     7321 gtctgatggt tgcgctggtc aatgtatatg ttctgctgct ggtactatct gccctgattg
     7381 ctctgttggt ttccaggaac attactttgc ctttgagtct ggtggccggt acatttcgaa
     7441 gcgtacaact gggaaaaaag aacgagccgg taagctggcc gcacaaagat gagatcgggc
     7501 tgctggtaac cgagtataac aagatgctgg aacagctgga acaaagtgca cgtttgctgg
     7561 cgcaatctga aagagaatct gcctggagag atatggccaa gcaggtagct catgagatca
     7621 agaatccact tacaccgatg cgtttaagca tacagcacct ccagcgggct atagagagcg
     7681 gacgtccgga tgtagaggaa ctgacctccc gcgtggcgaa aacattgatg gagcagatcg
     7741 acaacctgtc tttcattgca tctgagttca gcaacttcgc ggtcatgccg aaggccgtga
     7801 acgaaccgat agagctgggc aggctgctga gcaatgtcag ttccttgttt gatgaaacag
     7861 agcgcacccg catcactttg gagttgcccg aagcagaaat gatcatctgg gcagaccgga
     7921 atcagatctt gcgagtattc aataatctgg tgaagaacgg gctgcaggcc attccggaag
     7981 acagagaagg cagcatgatc attcgtcttt ctgccagtca gggaagggca ctggttactg
     8041 ttcaggataa cggggcagga attccggatg atatcaaaga ccaggtgttt gtacccaact
     8101 ttacaaccaa gagttccggc atgggtattg gactggcgct ctcgaagaat attgtggagt
     8161 ctgctggtgg ttacatctgg tttgaatcag agccgggttc aggaacaacc ttttttgtag
     8221 agtttcctct gtatgacggc cggcaggaag agggggctga tacctgacac ggtttcacta
     8281 cttttattcc atgaagttgt tcagcggctg gcgaacaggc atttttttta cggtggtgta
     8341 cctgtatatc atcggttcat ttgtatggtg gtggtggttg ctccagtcta acaacaaaga
     8401 gaactatgaa accctgatac aacgtgagcg catcacctac atgagttatg gccgctctga
     8461 agaggggttt tatgacaccc cttttttcct cgaactggaa cgtgattatc accggaaaac
     8521 caccatgctg attggtgaag gaatagtgtt tatttctctg atctctcttg gttttgtcag
     8581 gctgcggaac accttccgtg aagagctggc tcttacccgt cagcaaaaca atttcctgtt
     8641 gtccattaca catgagctga aatcgccact ggcatctctg aagctttcca tgcaaaccat
     8701 gcaaaagcat gatctggaaa gagacaaagt gcgcaggctg gccgacattt ctatgaatga
     8761 tgtggatcgc ctggaagcgc tggttgagaa catattgctt gccagcaaga tggacagcag
     8821 tgacttcagt atttcacgtg acccctttct gctgtcagat gtggccaggc agatatttga
     8881 cagaacggca gacaaacatg caaccgaacg aatcttttct gcagacatag caccggagat
     8941 ttatttgctg ggtgatcgtt taatattttc atcagtcata tataatttgc tggaaaatgc
     9001 aatcaagtat tccaggcagg gcgatagggt ggagctcctt ctcaggcaaa ccaaagacca
     9061 tattgtactg gaagtaagag atacaggcat tgggattgca gatgaggaga agccaagggt
     9121 ttttcaaaga ttttaccggg tgggcagaga agagacccgc actaccaaag gaaccggtct
     9181 gggtttgttc attgtaaagc aggccgtgca ttttcaccag ggtactatat cagtgagcga
     9241 taacctgcca cagggcacca ggtttatcat tcatattccg gttgaaaagt aaaatctgtc
     9301 aaatccatga caggcatgac aaaactttga tgttactgct gcgtattttt gaataggcct
     9361 taggctatat ttcaacacac cttaaaacca aagcttatgc agtacaggat cctgttggta
     9421 gaagatgaag atcacattcg tgaaaatctc aaactgaatc tcgaattgga agattatgaa
     9481 gtggttgccg ttgacaatgg cagcagggct atcaaagact tccgcgaaca acgtttcgat
     9541 ctggtcatcc tggacgtaat gatgcctgaa atggatggtt ttgacgtgtg tgaacagatc
     9601 cggctggaag acctgaatac gcctatattg tttctgaccg caaaagatac ctcacaggat
     9661 aaaattcagg ggctcaagac aggtgcagat gattacatga ccaagccgtt caacctggaa
     9721 gagctgttac tgcgggtacg caatctgatc aggcgcagcg ccaagacccc tgcagcgaga
     9781 aaagaatttg tggagtacac tttcggagga aacaccatca acttcatgac ctatgaagcc
     9841 cagggtgttg acggcaggca caaactgacc aacaaagaac tcaaactgct gaagctgctg
     9901 atagacagga aaggtgaggt ggtgtccagg caacatattc tccaaacggt atggggctat
     9961 gatgtctttc cttctacccg gaccattgac aattttattc tggcgttcag gaaatatttt
    10021 gagcaggacc ctaaaaatcc tacctatttt cattctataa gaggggtggg ttacaagttc
    10081 acagatgccg aagcataaag catgtaaaca gacagccggc agcaaactgc cggctgtcaa
    10141 aaccaaccaa actaatgata aaccttacct gatttcgatg ttctttgtgt gcacttcttt
    10201 cgattctttc ttaggaatgg taagtttcag cagaccatca agataaacag cctcaatgtt
    10261 gtcttcggta acattttccg gcagggtgaa tgagcggctg aaggattcaa aactgaactc
    10321 tctgcgggta aagttccttt tctcttctac ctcttcattc tttctctctg cactgatggt
    10381 tatgagaccg ttttcaacgc ctactgaaaa atcttctctc ctcataccgg gaacggccat
    10441 ctcgatcaca aactgtgtgt cgttctcttc aatgttcact gccggcatcc tttctggtgt
    10501 gaagacatcc tttccaaaga acctgtcacc actaaagaaa tctgataaca gggtcggtcc
    10561 aaacgggaat tcaatccctt ttcttttgat cagtgccatc gttgtgcttt ttagaacgtg
    10621 accaatcaag tacaggtcaa aactagatct ccaccacagc acttgctatg acagacatca
    10681 ttgaaaaaag gtgatatggg tcaggatccg gaaaacatcc caaggtcaat caaaacatgt
    10741 ccatgccacc acctccgttg ttgtcccagt ctccctggcc accacggcgg cggttgttgt
    10801 cttgctgccc gaatttgtag ctcaggcgaa tactgacagc ctgcgactct cgcttgcgcc
    10861 tgaattcctg actgaaattg aaaccttcat tggtcacgta gaactcacgg gtatcaaata
    10921 catcacttac agtcaggttg acactcagct tgttgctgaa aaaatctgaa cgaatgccca
    10981 gatcaataaa tgtgaacggg cgaggttgcc cttgcgccat tacccagggc ctccagtagc
    11041 tgtatgtggc ctgcagtgat gtctgcttca gtactttgaa gaacatcatt acccgtgcat
    11101 tgtaattggt cagcgcattg ctgagaccgg cttcaatatt ggaagcatcc agaatggttc
    11161 ggtttacgtt cagactggaa gtggcagacc accacggaaa gaattcgttt cttaccacga
    11221 attcagcacc gtatgaatct tctgtgctga aattctcaaa ggtggtgcga ctcaggccgt
    11281 tgctgtcaac agttgtatag gcagaaaact ctccatcagt gtgtttgtag aatacagaac
    11341 tgctcagggt gtgtttgtcc cagatgcggg taagtcctaa ttcaaagctg tgttcatctt
    11401 ccggaagaag aaatggattg ccttgtctgt aggtgtaagg atcagaaaat tcaggaaacg
    11461 gattcaggaa aaaagaacgg ggacgattga tccttctgct gtaactggca ttggtttcta
    11521 cacctttttt gatctcatat ttaagatgaa ctgaaggaaa cagtcggaaa taatctttat
    11581 tgaaagattg gccggtggtc acaagctcag aagtcaggaa ggtttgttct gccctcaggc
    11641 cgagctgata acccagctta ccaatgctgg agttccagat ggtgtatgct gccagtacct
    11701 gctggtcaaa acgaaaccgg ttggatatgc tgtcgtcatt ggcaaataca cctgattctc
    11761 cgtcaaagct ttcagacaag atatcattgt ctctgaactc acgggtaaac cgggctcctg
    11821 cttccagctg attaccgttc ttgaatggat gtatataatc agaattgatg tttatgtcct
    11881 ggttgcggtc atcagacaat acctgctggt tgatgcgtgc tgccagatct tctgcccctg
    11941 aattgtcata ggtgaattct gtgtatccgg tataaccatc gccaaaacca ttgctgaaaa
    12001 aagcatcggt ggtccacaga tgtttggggt tctcccaggt cttcctgtag ttcaggccaa
    12061 tgttgtagtt gtggttatcg gaagaaccgt ctccttgtcg ctggttgtac tgacttaaca
    12121 ggctgtcatt atcgtagaaa aagtagttgt tcaggtcatc attattattg tttccggtgc
    12181 tgtacaaggc agaaaaacca atggtattcc tgtcatttat ataatagtcg gcacctccgc
    12241 gaatattatg accagaccgg ttatcggttg attcgccgtc agaattgtaa taagaggtag
    12301 agtcattatc ctcatagaac accttccggc ttacctcgct ccaaccgtct cgggtacggt
    12361 tatcgtaaga atagctgcca aaaagattcc atttcttgtc gcggtaattc agggtcagtg
    12421 aaccatcgta acccggaaag gtgcttcccc ccacagtggc cacaccgttc agcccaacct
    12481 ttttgttctt tttcaggact atgttaatga tccctccgcc gccttcggct tcataccttg
    12541 cagaggggtt ggtgaccacc tctatgcttt caatactgct ggcaggtatc tgttccagga
    12601 tggccaccat gctggaaccc atgagtccgc tgggtttgcc gtcaatgaaa atgcgcacat
    12661 tagagttacc ccgcaggctc acattaccgt caatatcaac ggtgatactg ggaatgttct
    12721 gaagcgcttc agaagctgta cctgagctgg ctacaatgtc cttgtcaata ttatagacct
    12781 ttttgtcaat gctctgctgg aaataggatc gttcagctga gatctctaca ccttccagta
    12841 cagtggaaga gacgctgagt tcaatttttc ctgctttgta aaatggggca tcagaactca
    12901 gaacgaagtt ggtaaaatat ggatcgtacc caaggaactc cacctttaaa aaataacctc
    12961 cgttagacaa tccttcaatt cggaacatgc ctttctcatc tgttatcgtc ccggagcgca
    13021 ggctgctgtc agattcggcc agaatcgaaa ctgcggcaaa ttcaacaggg gtgcctgact
    13081 gtttttccag caccatcccc acaactgatg ctggtgatcc tcctgcgtaa ggaggttgct
    13141 gggctaaaac ggaaacagaa gatgcaaagg tcaacagacc tgacaggcac cagagtgtaa
    13201 ttgatctgat catggcagag ggtaaaatta cttaaatgct cactgaggct aaaggtttaa
    13261 aacgcatttt cggttcgttt aaggcttatt tagcccgttc ttttattact ttgcacacat
    13321 caaatctgta tcgtggaaga caaaagagaa aaactacttc gtttcaggca ggaagccctg
    13381 gccggaggag gtgagaaacg gatcgcttct cagcacaaga agggaaaact gactgcccgc
    13441 gagcgcattc agttattgat ggatccggat agttttgagg agataggtca gttgaaagtg
    13501 catcgttgtc atgattttgg catggagaat gagcgttatt acggcgatgg ggtagtgacc
    13561 ggatacggaa cggtaaacgg cagactgatc tatgtctatg cccaggattt tactgttttt
    13621 ggtggtagtc tgagcgaaac ccatgcagaa aagatctgcc ggatcatgga tctggccatg
    13681 cagaatggag ctcctgtagt aggtcttaat gattcgggtg gagcccgcat tcaggaaggg
    13741 gtcaactcac tgggcggcta tgccgatctc ttttaccgga acaccctcgc cagtggggtc
    13801 attccccaga tatcagccat catgggtccc tgtgcaggag gtgctgtcta ttctcctgcc
    13861 ataacagatt tcattctcat gaccgagggc aactcctata tgttcgtgac cgggcccaat
    13921 gtggtgaaga ccgtaacgca tgaggatgta agttttgaag aactgggagg tgccagtacc
    13981 cacagtacca aaagcggagt ggctcatttt acaggagaga atggagtgat ggtcattgag
    14041 caactgaaga agctgctgtc ctatattccg cagaactgtg aagaagaagc tccgatgaca
    14101 gcgtatcagc ctgcagatga atcaagacca aagctggggg cgatcatccc ggaaaaccca
    14161 aaccatccgt atgatatccg cgaggtggtc gatgaagtag tggatagcaa ttccttcttt
    14221 gaagtacaca aacaatacgc agccaatatc gtggtagggt ttgcacgcct gggcggtcgc
    14281 tctattggtg tggtagccaa ccagcctgca gtactggcag gggtgctgga tattgaagcc
    14341 tcgcgaaagg cagccaggtt tgttcgcttc tgcgattgct tcaacattcc tttgctggtg
    14401 ttcgaagatg tgcccggatt cttaccggga acagaccagg aatggaacgg catcatcacc
    14461 aacggagcca aactgctcta tgctttctgt gaagcaacgg tacccagggt gactgtcatc
    14521 acccgaaagg cttacggtgg agcctatgac gtgatgaact ccaagcatat cggagctgat
    14581 atgaattttg cctggcctac cgcagagatc gcggtgatgg ggcctaaagg tgcagccgag
    14641 atcatcttta aaaaagagat cgaagaagcc gacgacaaag aagccaaact gcaggaaaag
    14701 atcgatgaat atacccgcaa gtttgctgac ccgtatcgtg ctgcattccg tggttttgtg
    14761 gatgaggtga tcctccccga aaatacccgc gaaaaactca tcagggcttt tgacatgttg
    14821 cgcaacaaag ccgtcaaatt acccagaaaa aaacacggaa atattcccct ataaaactcc
    14881 atatggctgt aagaaaaacc acgatccgtc aggaagatct gaatttcctg gaacagtatc
    14941 tcaacaatcc gtcgccaaca ggaaatgaag caggcggaca aaagatatgg ctggaatata
    15001 tgaaaccata tatcgatgat cattacgtag ataactacgg gtctgtggca gctatcatca
    15061 atcccggaaa ggaattcagg gtagtgatag aagctcatgc cgatgagata gcctgggcgg
    15121 ttcacctgat caccgaagac ggattcattt atgtggagcg catcggtgga agtgatcata
    15181 tgatagcacc atccaaacgt gttaatatcc atacagataa aggagtggtg aaaggggtgt
    15241 tcggatggcc tgccattcat accagacatt atggaaagga agagtcgcct gaactgaaga
    15301 atatatttgt ggactgcgga tgttcctcta aaaaggaagt ggaaaaactt ggggtgcagg
    15361 tgggtgatct catcaccttc gatgatgact tcatggtgct caacaagaac atgtatgtag
    15421 gaagagcact ggacaaccgc atcggcggtt tcatgatcgc tcaggtggca cgtttgctga
    15481 aagagaataa gaataagctg cccttcacct tgtatgttgt caattcggtg caggaagaaa
    15541 taggactaag aggggcagaa atgatggcac attacatcaa gccacatgtg gccatcgtga
    15601 ccgatgtgac acacgacagc aatacaccta tgatcagcaa gaacctggaa ggcgatatac
    15661 gtgccggcag aggtccgagt ctgaccaaag gtcctgctgt acataacaaa ctgctgaagc
    15721 tggtgatgga tacggctgca gaaaaaaaga tcccgtttca acgtgatgtc gccagccgct
    15781 ccacaggcac agataccgat gcattcgcct acagcaacgg cggagtaccc agctgcctga
    15841 tcagcctgcc gctgcgttac atgcatacaa cggttgaatc agctcataaa gatgatgtgg
    15901 atgcggtgat acgcctcatc tacgaggtgc tgctgaagct gaacccgaag ttcaatttca
    15961 aatatctctg atcactcacc cagtagtcca gcaagtatct gagacaactg ctctgaccgg
    16021 atattctctg ctatgaccac tccgttggtg tccaggagaa aggatgccgg gataccggtc
    16081 acattgtatg cagccgctgc actggaattc catttttgta actgactgac atgtgagggc
    16141 cagctcagtt tatccgagcg aatggcctgc atccatgcat cccggttgtc atcgagcgat
    16201 acagaataca ctgtaaatcc cttgtcttta tattgctcat agagggcaac cagattcggg
    16261 ttttcctttc tgcatggagc gcaccagctg gcccagaaat ccagtagtac atacttaccc
    16321 cgaagagaag ataactgtat cggtttgccg tcaggatcgg gcaggttcag gtcagtggcc
    16381 gttttaccaa ggaaactatt ggctgcttcc tcatccatga tctgctggta ccggttcacc
    16441 ttgctggtca gttctctgac atacacattg ctggtatcgc cggttgcaat gatgggcgct
    16501 gctttttctt taatgatctc gaactgtctg tcatacaaaa gattctccag ggctacgatc
    16561 ttattggtga gctcttttgt agtatctgca aagttggcaa cataggcgta gaatgaatcg
    16621 atcatggcat ctttccggtc attcatgaca gcggtcagtg aatcacccaa tacggccgat
    16681 tgtgataaga cattattgct gaagtctatg atctccatat tcttgtttct tagttcatct
    16741 atgaaaccat tcagcaccag cgtggcagtg gaaccttcaa acagaggttt ggggtcgttg
    16801 gtggagccac ttatgtgtag cttttcatca tatatgttta acaataggta ataccttggg
    16861 ttgttttcca ttttgatacg acaaatgctt ctttcacgca ggtggccgct gaagataaag
    16921 tttccgtcag ggtctgcggt caccgtatct atagcagtga aggcaggtgt gccgttcatg
    16981 ttcgggaagg tcatggtttc cagcagcaga gtggcatttt caagaccacc gatggagcct
    17041 tccaccgtaa atgtcttgcc tccgttattg gtgcaagatg taatgccggc agtcattaac
    17101 catatggctg caattaggat gaataaggtc tgttttttca tgcttccagt tttttttgca
    17161 gcaaggcggt ggcaaccttt ggatctgcct ttcccttgct tcttttcatg acctcaccca
    17221 taaagagccc gatcagtcct ttttttccat tgcggtactc ggccacttta tcagggaatg
    17281 ccttcagggc ctcatctacc agctgttcta tgaatgcttc atcagtttcc tgtatcaggt
    17341 tcagtttttg tgccagtgca tcaggcgcag tgtcaggctc ttccacccat accggcagaa
    17401 gttgttgtgc tgccgcactg aaactcagtt ttccctcatc tatgagtgca agcagggcgg
    17461 ccagctgagc cggggcaaca ggaaaatcat ccatatctcg ggcacctgca ttcagccatt
    17521 cccgcacagg gccgagtatc cagttggcgg ctgctttgta acgtgtagta tgctggatca
    17581 gggcttcaaa gtaagtaacg acactctttt cagctgtaag cagggccgca tcataggtgc
    17641 ttaatcccag ttcctgctca tatcttttct gcagctcttt gggcagtgcc ggcatacctg
    17701 acctgatctc ttctaccata gctgcatgta tgcgtagtgg cggcagatct ggttccggaa
    17761 aataacggta gtcatgggct gcttccttac ttcgcatgga gaaagttgtg cccgcagctg
    17821 cgtcaaaact tctcgtttcc tgagagatgg tttcgccatt ctctatggct gctatctgcc
    17881 gttctatctc atggtcaatt gcccgcttca cgtttctgat agagttcatg tttttgacct
    17941 ccaccttcac accaaattca cttgctccct tttttcgaac gctgatattg gcatcacaac
    18001 gcaggctgcc ttcttccatg ttgccatcac agatggccag gtaacgtacc agtttgcgaa
    18061 tctctgtcag gtactcatag gcttcttcgc cggaacgcag gtcgggttca ctgacgatct
    18121 ctatcaaagg ggtacctgcc cggttca
//
LOCUS       DAOMAX010000020        18074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_326_length_18074_cov_4.650147, whole genome shotgun sequence.
ACCESSION   DAOMAX010000020 DAOMAX010000000
VERSION     DAOMAX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_326_length_18074_cov_4.650147"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..252)
                     /locus_tag="PK742_02300"
     CDS             complement(13..252)
                     /locus_tag="PK742_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75449.1"
                     /translation="MHNNSLFFVLVLVLVAALTSCSKSATCECTTKEVLDADGTVLSS
                     SKDTYKNYGNDPTCDDWESVETTDSTTITTSCVSI"
     gene            complement(279..1445)
                     /locus_tag="PK742_02305"
     CDS             complement(279..1445)
                     /locus_tag="PK742_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75450.1"
                     /translation="MKKHYYSAILTAAVSLLAVTTSGDAQAQGCVAIRSMTGEVASGN
                     NLLMKGDMQVGFGYRFFHSYKHFRGPHEESYRVGEGTDVRNDVNAFDLLYTYGITDRW
                     NATLDIPYTFNWRSSKYEHGNAPNGVDVRRSTNSHGIGDIRLQFDRWMIDPNSSDANG
                     NFSLGAGIKLPTGNFAYQDTFYYRGDEGENILKEVDQSIQLGDGGWGFSLQSQGFAKL
                     ESDIFFAYYSIYYLFTPGDTLSWEKRVDKIDAANEPAVNYSISDQFLVRGGVTYTPTS
                     WSGSAINLGFRFEGIPVEDVFGETNGRRRPGKVFSVDPGVTYVTGNHIFSLNVPIALY
                     RNRTQSVQDIERQQIDPGPSGDPRWGDAAFSDYTIFFNYGFRIFGKSHGDHNMM"
     gene            complement(1583..4219)
                     /locus_tag="PK742_02310"
     CDS             complement(1583..4219)
                     /locus_tag="PK742_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQU75451.1"
                     /translation="MAKKNEEKKRFKFKQMKVYGSLESFHNNSKNYRIVFDESEVSYV
                     NVEVQLYNILFDEEEWSAKIRVRASEYYSGKEYCNVEKELKAGIDQNIVYIREGWGTP
                     QPGWWKKGTYKWEVFLDDELIGTNYFYITNNGIVTPEHNPYFSINNIRLYESPRGNTP
                     KEERKYLSQFSKDTARYISIEMELTNLVSQEDYFPLELIFNFYNDVRQMKGSSYWFEH
                     ISDHRKQIILDVGYGSDAPGFWFADKYICEVLFMDNIVAIVPFEVADQDVALSGNQIW
                     MDAVSNDAVINDPQSVSNATSLEDARKELDELIGLESVKKEINELTTYLKFLSLRQQK
                     GFEEESKFNLNMVFTGNPGTGKTTVARMLGKIYRAMGLLSKDTVTEVGRVDLVAEFIG
                     QTAPKTKAIIEQARGGILFIDEAYALTDRGDDGKDFGREVIEVLIKEMSDGKGDIAFV
                     FAGYPKEMRKFLESNPGLSSRVGNIIHFPDYTPDELMAIADYHSRKRGVIIAPEARDF
                     IYMKIVEEYRNRDEKFGNARLMNSVIDEGKQNMALRIMRHEQPETLSNDDLSTITLPD
                     IQSIFGMLTGSGVEFPVDEALLNDALAQLRELVGLNEVKQEIDEIVKLVRYYKEIGKD
                     LRKSFSLHTVFTGNPGTGKTTVARILIKIFKALGILEKGHLVETDRKGLVAGYVGQTA
                     IQTDALIQNAMGGGLFIDEAYSLTEGGDGYGKEAVETLLKRMEDHRGEFVVIVAGYTE
                     EMFRFLESNPGLKSRFDKFIHFSDYSPEDLYVIAVNMFASENLQLQDAAAAHLQQYIK
                     QLCSTRNKYFGNARTMRKLVADTVHNQHLRMAGLPAEQRTDELIHTITLQDVDEFTMD
                     EVTPQKTSIGFKMSGGSNEQQP"
     gene            4337..5074
                     /locus_tag="PK742_02315"
     CDS             4337..5074
                     /locus_tag="PK742_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HQU75452.1"
                     /translation="MLAEISTNPPVSANKEACKKKTEDITEELDELQNRLYAEGKQSI
                     LIVLQGMDASGKDGLIRDVFRLINPQGVRVQSFKKPTEEEMDHEFLWRIHPHVPRKGM
                     IQIFNRSHYEDVLIQRVHAWVDMDTIKRRYDHINAFERLLQENGTRILKFYLHVSQKE
                     QHERLAERMEIPRKMWKYNPNDMKESEHWDEYMQAYEDVFRICSPDIPWTIVPSDKNW
                     YKEYLVARTIYDALKDMNPQYPGLPEG"
     gene            complement(5075..5974)
                     /locus_tag="PK742_02320"
     CDS             complement(5075..5974)
                     /locus_tag="PK742_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75453.1"
                     /translation="MSNSTLQKKLAAYSALATTAMFANQADAQVIYTDVDPDFIITEP
                     ADPPYTDILVMDINMDSSPEGTFAFSSFLFDGSCYTAGSMSFYFINYASVLLNDTSAG
                     VRILNEGEWINSSGNWNNDVFQQFMFSDFFNNTCDSDLEYPVIGGNWRQENEKYMGIR
                     FYDAAADLHYGYVRLTVLIDSVAGDAEVELIKVYGYAWESSADTPIMAGDGYQPDAIH
                     DLAVMELQIWPNPTSELLYVDQIPPGMQQARWLIHDLAGRLIRDTPLNGPVLSVADLQ
                     PGVYLLSLQDGDQPVGISRFIKQ"
     gene            complement(5967..8039)
                     /gene="scpA"
                     /locus_tag="PK742_02325"
     CDS             complement(5967..8039)
                     /gene="scpA"
                     /locus_tag="PK742_02325"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HQU75454.1"
                     /translation="MSRWQDKPLKHFPDWDKPPAEAEELAHTMPGMEPFIRGPYTTMY
                     VRKPWTIRQYAGFSTAEESNAFYRRNLAMGQKGLSVAFDLATHRGYDSDHPRVMGDVG
                     MAGVAIDTVEDMKILFDQIPLDTMSVSMTMNGAVIPIMAFYIIAAEEQGVQPAQLSGT
                     IQNDILKEFMVRNTYIYPPGTSMRIVSDIFKYTSKHMPRFNSISISGYHMQEAGATAE
                     IELAYTLADGLEYVRAGLAAGLDIDDFAPRLSFFWGIGMDHFREIAKMRAARLLWYRL
                     MSQFGPKNDKSLMLRTHCQTSGWSLTEQDPFNNVARTCVEALAAALGGTQSLHTNSLD
                     EAIALPTDFSARIARNTQLYLQHETGICRTVDPWGGSYLVENLTREIAEQAWQLIEEV
                     EALGGMAKAIEAGIPKLRIEESAARRQARIDTGKDIIVGVNGFVAEEDNNMELLEVDN
                     TAVRENQISRIQAVKATRDQTSVDAALKELADCAAGGEGNLLDLAVQAARVRATLGEI
                     SDAMESSFGRYTANVQAVSGVYQQEAGMDEHFQEASTLSNQFAEMAGRRPRILVAKLG
                     QDGHDRGAKVIATGFADLGFDVDIGPLFQTPEEAARQAIENDVHVLGISSLAAGHKTL
                     VPQVVESLRTQGRPDIMVVVGGVIPPDDHAFLTEKGASFVFGPGTVISLAAIDILRKL
                     IQEQTDHV"
     gene            complement(8020..9402)
                     /locus_tag="PK742_02330"
     CDS             complement(8020..9402)
                     /locus_tag="PK742_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQU75455.1"
                     /translation="MSIPANWEEHSFEDWKKKVDQELKGASFEETLQSDLYGIRLAPL
                     YTDRPAETSNEEEDILGEHEQSGMQWQATGSFRYMEGSVASILQEAFTEQISWMRISG
                     QVSLFLDQLAKEVPLPVTLCLEAESVPASEDEVLEWRDRLRKAKAHHPFLWSLEFDPI
                     GNWARTGAIRQAAHTFDHLYDLIIRLESDLTDTKFIRVDAAVAGEAGAGPLTEIAYAL
                     SAGSCYLKEMTERGMELYEAAHLLVFQFSAGTDHFAEIAKLRAFRTCWRNLLEYLDPE
                     FAYMEHPPVHVRISQVFHTQADVHNNLLRHTTAALSAILGGADVLEVPPHEEGIRGHR
                     LAGNIQRLLRYESRMDSYAKAASGSYYLEQLTGQVAEAAWKIFCVWEDAGGCLEQLRS
                     GQLQSAIQTDRLQLLNDHLSGKKVLIGVNKYVQETPELPQQKHVEFNEFTAILPWNLE
                     QAIRHEQVAG"
     gene            9507..10295
                     /locus_tag="PK742_02335"
     CDS             9507..10295
                     /locus_tag="PK742_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQU75456.1"
                     /translation="MQAENILTEVRDNTLYITINRPKALNALSIKTIVEIDEALEEYM
                     YHEDVKGVVITGAGEKAFVAGADISEFSDFTPEQGKTMSENGHDTFDKIESYPKPVIA
                     AVNGYALGGGCELAMACHMRIASENAIFGQPEVNLGLIPGYGGTQRLPELIGKGKALE
                     LLLTGDSINAAMALELGLVNDVVPQSELMKRVQEILQKIYTKGPVAVSKIIQSVNAHY
                     RSGSYVGDGYKTEISQFSACMDSEDFREGTAAFLEKRKPQFQGR"
     gene            10385..12664
                     /locus_tag="PK742_02340"
     CDS             10385..12664
                     /locus_tag="PK742_02340"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQU75457.1"
                     /translation="MPEKMRKKDALEYHASGRPGKIEVVPTKATKTQRDLSLAYSPGV
                     AEPCLEIAEDTDKVYQYTAKGNLVGVISNGTAVLGLGDIGPEASKPVMEGKGLLFKIY
                     ADIDVFDIELNCKDVEDFIRTVKAMEPTFGGINLEDIKAPECFEIERRLKAEMNIPVM
                     HDDQHGTAVISGAALLNALELTDRKLDEVKIVVNGAGASALSCASLFVELGVNRSNIV
                     MCDSKGVLNNRREGLDQYKQQFVTDKQVDTLAEAIVGADVFLGLSKGNVFTPDMLLSM
                     AKDPIVFALANPDPEIEYDLAIATREDVIMATGRSDHPNQVNNVLGFPFIFRGALDVR
                     ATGINEAMKVAAVRALAQLTHEQVPEFVNMAYNEKNLQFSRHYIIPKPVDPRLITRVA
                     PAVAKAAMETGVARKPITDWAAYNTDLEKRLGLDHQLIKGLVSKARNNPKRVVFAEAD
                     NYKILKAAQVAYDEGIAKPILLGNRDKIERIASENGLELHGMPIIDSHSDEMEDMRRE
                     FGELLFEKRRRKGLNYYEAKKIMRERNYFGAMMVELGQADALISGLTRQYPDTIRPAL
                     QIIGRQEGVNKVSGMYILLTRRGPIFFSDTTINFHPTTEELVDITVLTANAVRAFNIQ
                     PKIALLSYSNFGSVDNAETRRISKAVSLLKERYPGMVVDGEMQANIPFDNDLMRENYP
                     FADLIDKGVNTLIFPNLDAANISYKLLQTLGQAEAIGPVLLGLKKSVHILQLGSSIRE
                     IQNMIAIAVLDAQTKANNP"
     gene            12661..13260
                     /gene="ruvA"
                     /locus_tag="PK742_02345"
     CDS             12661..13260
                     /gene="ruvA"
                     /locus_tag="PK742_02345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQU75458.1"
                     /translation="MIAYIRGKIVSRNPASVVLDTGGIGYQLQISVHTYSSMPESGDC
                     LLYCYHYFREDGQSMIPVIYGFSSVEEKDMFIHLISVSGVGANTARMMLSSMSVADLK
                     QAIFTDNDGLIQKIKGVGPKTAKKIVIELKDRIGKSTGTETTSFVPSDNNIREEALSA
                     LAMLGFSRAAADKAIQAAQKATPELASVEELVKSALKNL"
     gene            13271..>18074
                     /gene="sprA"
                     /locus_tag="PK742_02350"
     CDS             13271..>18074
                     /gene="sprA"
                     /locus_tag="PK742_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQU75459.1"
                     /translation="MAGLRLFPKVALAGLTGLAVTISAFQQTAAPATLFVRSRPNIYL
                     PLPDSTDKDSTDTSGGQVSDYFADDQNAFGLDLPAGITPTLEYDPVTGDYIYSEKVGG
                     QYFKNPQYISFEDFLKAEQEKSITDYWVERSTAQSILGGSGAIPQIYTGSELLDRLFG
                     GSNVDIRPQGNIDLTFGGSFQNVGNPILTEDQRRQGGFDFNMDINANMLGQIGEKMKL
                     TFNYNTSATFDFENQVKLEYTGYEDEIIKKIEAGNVSLPLTTSLIPGTQSLWGIKTQL
                     QFGRLTMTNILSQQRSQSQSIQIEGGAQKREFEVFANQYDENRHFFLAHYMRDHYEPW
                     LSTLPFVNSPVNITRMEVWVTNTTGATQNTRDIVAFTDLGETDSTFWSVANGGTINVL
                     STDGLPSNNANDLYGKLVNDDNARNLNTVVSSLSDPAGQYKMQPVRDFEKVRMRKLNT
                     SDYSYNNQLGFISVNTSLQPDDVLAVAFEYTYLGQVYQVGEFTNNLPPGVDTSNVMFL
                     KMLKSTSAQVNIPLWDLMMKNVYSLGAFQVSPQDFQLDILYQDPGGGFKRYIPANNTN
                     VNGQPIITLLNLDRLNTNSDPFPDGVFDFVSGITINTNNGRIYFPVLEPFGSHLRSEQ
                     AFGTDPEVERYAYDELYSTTRVLAEQFPQYNRFVITGSYKSSVSSEIYLGAFNLPKGS
                     VTVTAGGQQLTENVDYTIDYSLGRLKIINESILNSGLPINVSFENNAFFGFQVKSLIG
                     SRFDYWINDNFTMGATVLRLSERPFTQKVNFGDDPIQNTMLGYDVNYTAEAPWMTRAL
                     DKLPFYDTKEMSSISFTGEVARFLPGHSKAIGKDDEGVIYIDDFEGSRSAYDLKFPFS
                     SWVIASTPQNAVDANGDVLFPEATLFDSLIYGMNRAKIAWYTIDPLFLREDNAARPDH
                     LTADDVSNHYLVEIQQQEIFPQADIAVTGLNNLSTFDLAYYPDERGPYNFDVSGTAFS
                     QGVNPDGSLINPASRWGGIMRSLETNDFEAANIETIEFWVMDPFDNLGPFGTQVTDGG
                     DLYINIGNVSEDILKDSRTFFENGLPKDGSTADLDATNWGLVPRTLPVNQAFDNDPNS
                     RENQDKGYDGQNDAQEAGFYADYLTQLQTILTPDALSVATADPSADNYHYYRGGDYDN
                     QELSIFDRYKRYNNPQGNSPTTESSPENYPTSATNLPESEDLNRDYTLNETEAYFQYR
                     IPITPDMMTTNPYVTDVVRAEHTFENGVVDSVTWYQLKVPINQFDARIGTIQDFRSIR
                     FIRMYMTGFEEPVVLRFARLELVRNQWRRYQFSLLEPGEYLPDDNSGETVFNVSSVSI
                     EENSGRQPFPYVLPPDVQREQTIGGGGNVSTFQQNEQALSMQVCPLKDGDSRAIFKSL
                     QLDLRRYGRLIMNVHGEPLVGSQTTDLQDGDLTVFVRLGSDFTNNYYEYEVPLKVSTL
                     PLPPGTADDDDVKRAVWETLIDFPLDSLISVKSERNNLEISPLVPYAVGGEGLPTYTV
                     IGNPDLGYVEEVMIGVRNPKSVSGSGEEYCAEVWVNELRLSEIDEQGGWAALARVDAQ
                     LADLGTMTLSGNMHTAGFGTLEQQINERYFDNFYQYAASMSLELGKFFP"
BASE COUNT         4594 a         4401 c         4467 g         4612 t
ORIGIN      
        1 gaaagtggag tatcagatgg aaacgcagga agtggtaatg gtggtagaat ctgtggtttc
       61 tacactttcc cagtcatcac aggtagggtc gtttccgtag ttcttatagg tgtctttgga
      121 actgcttagt acagtgccgt ctgcatcaag cacctctttg gtggtacact cgcaggtggc
      181 agatttggag caggatgtca atgcggcaac caataccagt accagcacga agaatagtga
      241 attattatgc atttctgttg tcttttgatt tgagtgaatt acatcatgtt atgatctccg
      301 tgagactttc caaaaatgcg gaagccgtag ttgaagaaga tggtgtagtc tgagaaggcg
      361 gcatcacccc agcgaggatc acctgaagga cccggatcga tctgctgacg ttctatgtcc
      421 tgtacgctct gcgtgcggtt gcgatataat gcgatgggta cgttcaggct gaagatgtgg
      481 ttgccggtta cataggttac gcccggatca acggagaaga cctttcccgg acgacgacgg
      541 ccattggttt cgccgaatac gtcttctacc ggaattcctt caaagcggaa acccaggttg
      601 attgcagaac ctgaccagct ggttggtgtg taggttacac caccacgaac caggaattgg
      661 tcagagatag aatagttgac agcaggttcg ttggcagcat cgatcttatc taccctcttt
      721 tcccagctca gcgtatcgcc tggtgtgaac aggtaataga tgctgtaata ggcaaagaag
      781 atatcagatt ccagtttggc aaaaccctgg ctttgaaggc tgaagcccca gccgccatcg
      841 cctaactgaa tggactgatc cacttctttc aggatgttct caccttcatc acctctgtaa
      901 tagaaagtgt cctggtaggc gaaatttccg gttggaagtt tgatgccggc acccaggctg
      961 aaattgccgt tggcatctga gctgttcgga tcgatcatcc agcggtcaaa ctgcagacgg
     1021 atatcgccaa tgccatgaga attggtagag cgacgaacgt ccacaccgtt tggcgcattg
     1081 ccatgttcat acttagagga cctccagttg aaggtgtagg gaatgtccag tgtggcattc
     1141 cagcggtcag tgatgccata ggtgtacagc aggtcaaagg cgttcacgtc attgcgtaca
     1201 tcggttcctt cgcctacccg gtaagactct tcgtgcggac cacggaagtg cttataggag
     1261 tggaagaaac gatagccaaa gccgacctgc atgtcgcctt tcattaacag gttgtttccg
     1321 gaagctactt cgcccgtcat ggatcgaata gctacgcaac cctgtgcctg tgcatcgccg
     1381 gatgtggtta cagccagcaa actaacagca gcggtgagga tagcagagta gtagtgtttt
     1441 ttcatctgaa tggattagaa tgcgaaaatc catagaacag ccaagctgct gttaatcaga
     1501 cgatagaaag aaaacctaac aaatgctaaa caagctcaat gtaaagcaaa taaattgtaa
     1561 aaacgttgat atggtgcaat gatcagggtt gctgttcatt agatccacca ctcattttaa
     1621 aaccgatcga ggtcttttga ggtgttactt catccattgt gaactcatcc acatcctgca
     1681 gtgtgatggt atggatcagt tcatctgttc tttgttctgc aggcagacct gccatgcgga
     1741 ggtgttggtt atgcacggta tctgcaacca gtttacgcat ggtgcgtgca ttgccaaaat
     1801 acttattgcg ggtactgcat aattgtttga tatactgctg cagatgtgct gctgctgcat
     1861 cctgtaattg caggttctct gatgcgaaca tatttactgc aatgacgtac agatcttcgg
     1921 gcgagtaatc gctgaagtgt atgaacttat cgaatcgtga tttcaaaccg gggttggatt
     1981 ccagaaaacg gaacatctct tcggtataac cggccacaat gaccacaaat tcaccgcgat
     2041 gatcttccat ccgcttcagc aacgtttcca ctgcttcttt gccatagcca tcgccacctt
     2101 cagtcaaaga gtaggcttca tcaatgaata gcccgccgcc catagcattt tgtatcaggg
     2161 cgtctgtctg aatggcagtc tgcccaacat agcctgccac cagtcctttc cggtccgttt
     2221 caaccaggtg tcctttttcc aggatgccaa gtgccttaaa gatcttgatc aggatgcggg
     2281 ccacagtggt tttgcctgtg ccgggattac ctgtaaaaac ggtatgcaga gagaaggact
     2341 ttctcaggtc tttaccgatc tccttatagt atcgcaccag tttgacgatc tcatcgatct
     2401 cctgttttac ttcattcagt cccaccagtt ctctgagctg tgcaagtgca tcattcagca
     2461 gggcttcatc taccggaaat tctacaccac tgcctgtcag cattccaaag atcgattgaa
     2521 tgtcaggcag ggtgatggtt gacaggtcgt cgtttgacag ggtctcgggt tgttcatgcc
     2581 gcatgatacg cagggccata ttctgtttgc cttcgtctat gacagagttc atcagcctgg
     2641 cattgccaaa tttctcatcg cggttgcggt actcttccac gatcttcata taaatgaaat
     2701 cacgagcttc cggtgcgatg atcacgccgc gtttcctgct gtgataatcg gcaatagcca
     2761 tgagttcatc cggtgtgtag tcaggaaagt gaatgatatt gcccactctg ctggagagcc
     2821 ccgggttaga ctccagaaat tttcgcatct ctttcggata accggcaaat acaaaagcta
     2881 tatcgccttt gccatcactc atttccttga taagtacttc aatgacctcg cgaccgaaat
     2941 cctttccgtc gtctccgcga tcggtcagtg cataggcttc atcaataaaa agaatgcctc
     3001 cgcgggcttg ttcgatgatg gctttggttt tgggagcggt ttgcccgatg aattccgcta
     3061 ccagatccac acgaccaact tctgttactg tatccttgct cagcaaaccc atggcccggt
     3121 agatcttacc cagcatgcgt gccacggtgg tcttgcctgt accgggatta ccggtaaaca
     3181 ccatgttgag attgaatttg gattcttctt caaatccttt ttgctgacga aggctgagaa
     3241 acttgagata ggttgtcagt tcattgatct ctttcttgac agattcaagg ccaatcagct
     3301 catcgagttc ttttcgtgca tcttccaggc ttgtggcatt gctgactgac tgcggatcat
     3361 tgatgaccgc atcgttggaa acagcatcca tccagatctg gttgcccgag agggctacat
     3421 cctgatctgc cacttcaaat ggaacaatgg ccacaatgtt gtccatgaac aacacctcgc
     3481 agatgtattt atcagcaaac cagaatcccg gtgcatccga gccgtaaccc acatccagta
     3541 tgatctgttt gcgatggtcg ctgatgtgct caaaccagta tgagcttccc ttcatctggc
     3601 gcacgtcatt gtagaagttg aagatgagct ccagcggaaa ataatcttcc tgacttacca
     3661 ggttggtcag ctccatttca atactgatgt aacgggcggt gtctttggag aactgactca
     3721 ggtatttccg ttcttccttt ggtgtgtttc ctctggggct ttcgtataac ctgatgttat
     3781 tgatactgaa atacgggttg tgttcaggag ttacaatgcc gttgttggtt atatagaaat
     3841 agttggtacc tatcagttca tcgtccagaa atacctccca tttgtaggtg ccttttttcc
     3901 accatccggg ttgtggtgtt ccccagcctt ctctgatata gacaatgttc tggtcaatgc
     3961 cggccttgag ttccttttct acattacaat actctttccc tgaataatat tcagaagcac
     4021 gtacccggat cttggcagac cattcttctt catcaaacag gatattgtac aactggacct
     4081 ctacattgac ataactgacc tcagattcgt caaatacgat acggtagttc ttactgttgt
     4141 tatggaagct ttccagcgat ccatacactt tcatctgttt gaatttgaat cgcttcttct
     4201 cttcgttctt cttggccatg gctgatttga ataaggaagt caaatatagg gtacatagtg
     4261 aattgaatac tgcttacagg caatggatca cggtcaaatg ccggtttttt ctcattttta
     4321 ttccaaaata tcgtgtatgc tggctgagat atctaccaac ccacctgtga gcgccaacaa
     4381 ggaggcgtgc aagaagaaaa cggaggatat caccgaagag cttgatgagt tgcagaacag
     4441 gttgtatgca gaggggaaac aaagcatact cattgtgctt caggggatgg atgccagcgg
     4501 aaaggacgga ctgatcagag acgtattcag gctgatcaat ccacagggtg tcagggtgca
     4561 gtcattcaag aagcctacgg aagaagaaat ggatcacgaa ttcctttggc gcatccaccc
     4621 tcatgtgcca cgtaagggca tgatccagat ctttaaccgg tctcattatg aagatgtact
     4681 cattcagcgg gtgcatgcct gggtagatat ggataccatc aaacgacggt atgaccatat
     4741 caatgcgttt gaacggttgc tgcaggagaa cggcacacgc attctgaaat tctacctgca
     4801 tgtttcgcag aaagaacagc acgaacgtct ggcagagcga atggaaattc ccagaaaaat
     4861 gtggaagtat aatcccaatg atatgaagga aagtgaacac tgggatgaat acatgcaggc
     4921 ttatgaagat gtgttcagaa tatgcagccc tgacattccg tggaccatcg ttccgtcaga
     4981 caagaactgg tataaagagt acctggttgc cagaaccatc tatgatgcgc tgaaagatat
     5041 gaatccgcag taccccggtt tacccgaggg ttgatcactg tttgatgaac ctggaaatgc
     5101 ccacgggctg atcaccgtcc tgcagagaca acaggtacac gcctggttgc aggtcggcaa
     5161 cagacagtac cgggccgttc aacggagtat ctcttatcag ccttccggcc agatcatgta
     5221 tcagccagcg ggcttgttgc ataccaggcg gaatctggtc aacatacaac aactcagatg
     5281 tggggtttgg ccagatctgc agctccatga cagccaggtc atgtatggca tccggctggt
     5341 agccatcgcc ggccatgatg ggtgtatctg ccgatgattc ccaggcatac ccgtacactt
     5401 tgatcagttc cacttctgca tcgccggcta cgctgtcaat cagtacggtt aacctgacgt
     5461 aaccgtagtg cagatctgca gcagcatcat aaaagcggat gcccatgtat ttttcatttt
     5521 cctgcctcca gttacctcca atgaccgggt attccagatc agaatcgcag gtattgttga
     5581 agaaatcaga aaacatgaat tgctgaaaaa cgtcgttgtt ccagtttcct gatgaattga
     5641 tccattctcc ttcattcagt atgcgaacac ctgccgaggt gtcattcagc agtaccgatg
     5701 cataattgat gaaatagaaa ctcatggatc ctgctgtata acaactgcca tcaaacagga
     5761 aactggagaa ggcaaaggtg ccttccgggc tgctgtccat attgatatcc atgaccagta
     5821 tatcagtata aggtggatca gccggttctg tgatgatgaa atctggatct acatctgtat
     5881 agatgacctg tgcgtctgcc tgattggcaa acatggcagt tgttgccagt gcagaatagg
     5941 cggccagttt tttttgcaac gttgagttag acatggtcag tttgttcttg tatgagttta
     6001 cgcagaatat ctattgcagc cagactgatg accgttccgg gaccgaatac aaagctggca
     6061 cctttttctg taaggaatgc atggtcgtcg ggcggaatta cgccccccac tactaccatg
     6121 atgtcgggtc ttccctgggt gcgcaatgat tccacaacct gtggtaccag ggttttatgt
     6181 cctgcagcaa gagatgaaat gccaagaaca tgcacatcat tttcgatggc ttgcctggca
     6241 gcttcttctg gggtctggaa caaagggcct atatccacat caaatccgag atctgcaaag
     6301 cctgtggcaa tgaccttggc accgcggtca tgtccgtcct gcccgagttt ggccaccaga
     6361 atgcgggggc gccgtccagc catttctgca aactggttgc tcagtgtgct tgcctcctga
     6421 aaatgttcat ccatgccggc ttcctgttga tagacgccgc ttactgcctg tacgttagcg
     6481 gtatagcgac cgaatgatga ttccatggca tcagagatct cgccaagtgt agcccttacc
     6541 ctggcggcct gcacggcaag atccagcaga ttcccttctc ctcctgctgc acaatctgcc
     6601 agttctttca acgcagcatc tacagatgtc tgatcgcggg tggctttaac tgcctgtatc
     6661 ctgctgatct gattttcacg aacggcagta ttatccactt ccagtaattc catattgttg
     6721 tcttcctccg caacgaaacc gttcacgcca acaatgatgt cttttccggt atctatccgt
     6781 gcttgtctcc gggcagcaga ttcttctata cgcagtttcg gaatgcccgc ttcaattgct
     6841 tttgccatgc cgcccagtgc ttccacttct tctatgagtt gccaggcttg ttctgcaatt
     6901 tccctcgtca gattttccac cagataagat ccgccccatg gatcgaccgt cctgcatatg
     6961 ccggtttcat gctgcagata cagctgtgta ttccgggcaa ttcttgctga aaaatctgtt
     7021 ggcagggcga tggcctcatc cagcgaattg gtatgcagag actgggttcc gccaagcgct
     7081 gctgccagag cttccacgca ggtacgggct acattattga agggatcctg ctcggtaaga
     7141 ctccatccgg aggtctggca atgggtgcgc agcatgagcg acttgtcatt cttcggaccg
     7201 aactgcgaca tcagcctgta ccacaacaaa cgggcggccc gcatcttggc tatctcacgg
     7261 aaatgatcca tgcctattcc ccagaagaat gataatctgg gagcaaaatc atcaatgtcc
     7321 agccctgctg ccagccctgc ccgaacatat tccagcccgt cggcaagggt gtatgccagt
     7381 tcgatctctg ctgtggctcc cgcctcctgc atatggtaac ccgaaatgct gatggaattg
     7441 aagcgaggca tgtgcttcga ggtgtatttg aaaatatcag atacgatgcg catgctagtt
     7501 ccgggcggat agatataggt gttgcgcacc atgaactcct tcaggatatc attctgaatg
     7561 gtgccgctta gctgagccgg ttgtacgccc tgttcttctg ctgcaatgat atagaatgcc
     7621 atgatgggaa tgacggcacc attcatggtc atgctcacac tcatggtatc gagcgggatc
     7681 tggtcaaaga ggatcttcat atcctccact gtgtcaatgg ccacgccggc catgcctaca
     7741 tcgcccatga cccttgggtg atcactgtca tacccgcggt gggtagccag gtcaaaggca
     7801 actgaaaggc ccttctgccc catggccagg ttacgccggt agaaggcgtt gctttcttct
     7861 gcagtactga agccggcata ctgccggatg gtccagggtt tgcgcacata catggtggta
     7921 tagggccctc tgataaaggg ttccataccg ggcatggtat gtgccagttc ttccgcttcg
     7981 gccggaggtt tatcccagtc aggaaagtgt ttcaatggtt tatcctgcca cctgctcatg
     8041 tctgatagct tgttccagat tccacggtaa tatggccgtg aattcgttaa attctacatg
     8101 tttctgttgc ggtaattccg gtgtttcctg gacatactta ttgacaccta tcaggacttt
     8161 ttttccgctc agatggtcat tcagtaattg cagtctgtca gtttgtatgg cagactgaag
     8221 ctgtcctgat cgcaattgtt ccaggcaacc gccggcatct tcccaaacac agaaaatttt
     8281 ccaggcggct tctgccacct gaccggtcag ttgttccaga taataactac ctgatgcagc
     8341 tttcgcataa ctgtccatgc ggctttcata acgaagcagg cgttggatgt tgccggccaa
     8401 tctgtggcct cttatacctt cttcatgcgg cggcacctcc agcacatcag ccccgcctaa
     8461 tattgccgag agtgcagctg ttgtgtggcg aagcagattg ttgtgtacat ctgcctgcgt
     8521 atggaatacc tgtgatatgc gcacatgcac cggcggatgt tccatatagg caaattcagg
     8581 atccaggtat tccagcagat tgcgccagca ggtgcggaat gcacgtaact tggcgatctc
     8641 tgcaaaatga tctgttcctg cactgaattg gaaaaccagc agatgagccg cttcatacaa
     8701 ttccattccc cgttccgtca tttctttcag ataacaggaa cctgcactca gtgcataagc
     8761 tatttcagta agggggccgg caccggcttc tccggctaca gcagcatcca cccgaatgaa
     8821 ttttgtgtct gtcagatcgc tttccagtcg aatgatcaga tcatataaat gatcaaaagt
     8881 atgagcagcc tgacggatcg ctccggtcct ggcccagttt cctatagggt caaactccag
     8941 tgaccagaga aaagggtggt gggccttggc ctttctaagt ctgtcacgcc attccaatac
     9001 ttcgtcttca ctggcaggaa cagactcagc ttccaggcac aaggtaaccg gcaaaggcac
     9061 ttcttttgct aactgatcta agaaaagaga tacctgcccg ctgatgcgca tccaggagat
     9121 ctgttctgta aaagcttcct gcaggatgga tgcaacagat ccttccatgt acctgaatga
     9181 accggtagcc tgccattgca tgccggattg ttcgtgctct cccagtatat cttcttcttc
     9241 attgctggtt tctgccggtc tgtctgtata gagtggcgcc agtctgatac catacagatc
     9301 actctgcagc gtttcctcaa aggatgctcc cttcagctcc tggtcaacct ttttcttcca
     9361 gtcttcaaag ctgtgttctt cccagttggc gggtatagac atgttgcaaa gatagagcag
     9421 cctgccggca tgcacatgac tttggtcaac ctacaccggt ctgtgtgccg cacagtgtgt
     9481 acttttgtac ccttcaaaag cccgatatgc aagcagaaaa catcctgaca gaagttcgag
     9541 ataacaccct gtacatcacc atcaaccggc ccaaggcact gaatgcttta agcatcaaga
     9601 ccattgtaga gatcgatgaa gccctggaag aatacatgta tcatgaagat gtgaaagggg
     9661 tggtcataac cggtgctggt gagaaagcct ttgttgcagg ggcagatatc tctgaattca
     9721 gtgattttac acctgaacag ggcaagacca tgagtgagaa tggtcatgat acctttgaca
     9781 agatcgaatc ataccccaaa ccggtcattg cggcagttaa cggctatgca ctgggtggtg
     9841 gttgcgagct ggcaatggcc tgtcatatgc gcatagcttc tgaaaatgcc atcttcggcc
     9901 agccggaagt aaacctcggc ctgattcccg gttacggtgg tacccagcgt ttgccggaac
     9961 tgataggcaa aggcaaggcg ctggaactgt tgctcacggg cgacagtatc aatgctgcca
    10021 tggcacttga actggggctg gtcaatgatg tggtaccgca aagcgagctg atgaagcggg
    10081 tgcaggagat cctgcagaaa atttacacaa aaggccctgt ggcggtttcc aagatcatcc
    10141 agtctgtgaa tgcgcactac cgcagcggca gttatgtggg tgacgggtac aagaccgaga
    10201 tcagtcagtt ttctgcctgt atggactcag aagatttcag agaaggaacg gctgctttcc
    10261 tggaaaaacg caaacctcag ttccagggcc gctaagccgg gaaagagttc ccatttcagc
    10321 agggtttccc tacctttgtt cccccgcagt aagggcctgt gccgcctgca gaaaacaaca
    10381 ccgcatgccg gaaaaaatga ggaagaagga tgccctggag tatcatgctt caggacgacc
    10441 tggaaaaata gaggtggttc ctaccaaagc cactaaaaca caacgagatc tgtcactggc
    10501 ctattctccc ggggtagcag agccctgcct ggagatcgca gaagataccg acaaagttta
    10561 tcagtacaca gccaagggca acctggtagg tgtgatcagt aacggaacgg ccgtactggg
    10621 gctgggtgat attggcccgg aagcatccaa gccggtcatg gaaggaaagg gtctgctgtt
    10681 caagatctat gcagatattg atgtctttga tatagaactg aactgcaagg atgtggaaga
    10741 tttcatccgt acggtcaagg ccatggaacc aacctttggc ggaatcaacc tggaagacat
    10801 caaagcaccg gaatgttttg agatcgaacg ccgcctgaaa gccgaaatga acattcctgt
    10861 catgcatgat gaccagcacg gtaccgcagt gatcagtggg gctgctttgt tgaatgccct
    10921 ggaactgaca gacaggaaac tggatgaggt gaagattgta gtgaacggtg ccggggcatc
    10981 tgccttgtct tgcgccagtc tgtttgttga actgggagtc aaccgcagca acattgtcat
    11041 gtgcgattcg aaaggtgtgc ttaacaacag gagggaaggc ctggatcagt acaagcagca
    11101 gtttgtgacc gacaaacagg tggataccct ggcagaagca attgtaggtg ccgatgtttt
    11161 cctggggctg tctaagggta acgtgtttac tccggatatg cttctgagta tggcaaaaga
    11221 ccccatcgta tttgcactgg ccaacccaga tccagagatc gaatatgacc tggccatcgc
    11281 cacccgtgaa gacgtgatca tggccaccgg ccgaagcgac caccccaatc aggtgaataa
    11341 cgtacttggt tttccattca tcttccgcgg ggcactggat gtgcgggcca ccggcatcaa
    11401 tgaagctatg aaagtggctg ctgtcagagc gctggcacaa ctcacgcatg agcaggtgcc
    11461 ggagttcgtg aacatggcat acaatgaaaa gaatctgcag ttcagcaggc actatatcat
    11521 accgaaaccg gttgacccga ggcttatcac acgggtagct ccggctgttg ccaaagctgc
    11581 catggaaacc ggtgtggccc gcaagcccat cactgactgg gctgcataca atacagacct
    11641 cgagaaacga ctcggactgg atcatcagct catcaaaggt ctggtcagca aagcaaggaa
    11701 caatccgaaa cgggtggtct ttgctgaagc agataattac aagatcctga aggcagccca
    11761 ggttgcctat gatgaaggca ttgccaagcc tatactgctg ggaaacagag ataagatcga
    11821 gcgtattgcg tcagagaacg gtttggagct gcatggtatg cccatcattg acagtcacag
    11881 cgacgagatg gaagatatgc gcagagagtt tggcgagctg ctttttgaaa aaaggcgcag
    11941 aaaagggctc aattattatg aagccaaaaa gatcatgcgt gagcgcaact acttcggtgc
    12001 catgatggtg gagctggggc aggcagatgc actcatctcc ggcctgacac ggcaatatcc
    12061 tgataccatt cgtccggcac tgcagatcat cggccggcag gaaggggtca ataaggtctc
    12121 gggtatgtat attctgctta cccgtcgcgg accgatcttc ttttcagaca cgaccattaa
    12181 ctttcatccc accacagaag aactggtgga cattacagta cttaccgcca atgcggtcag
    12241 ggcttttaat atccagccga agatcgccct tctgtcttat tccaattttg gatctgttga
    12301 caatgcagag acccgtcgaa tcagcaaagc cgtcagctta ttgaaagagc gctatccggg
    12361 tatggtggtg gatggagaga tgcaggccaa tattccgttc gataatgatc tgatgcggga
    12421 aaattatcca tttgcagacc tgattgacaa aggggtgaat acccttattt tcccgaatct
    12481 ggatgcggcc aacatatcct ataagttatt gcagaccctg ggtcaggctg aggccattgg
    12541 tccggtgctg ctgggattaa agaaatcagt acatatcttg cagctgggca gttccatcag
    12601 ggagatccag aatatgatag ccattgctgt tctggatgca cagaccaaag caaataatcc
    12661 atgatcgctt atatcagggg aaagatcgtt agccgaaatc cggccagtgt tgttctggac
    12721 accggtggca taggttatca gctgcagatc agtgtgcata cctattcttc tatgcctgaa
    12781 tcgggagact gcctcctgta ctgttaccac tattttcgtg aagacggcca gagcatgata
    12841 ccggtcattt acggattcag cagtgtggaa gagaaagata tgttcataca cctcatcagt
    12901 gtgagcggag tgggggccaa tacagcccgg atgatgctgt ccagtatgtc tgtggcagat
    12961 ctgaaacaag ccatatttac tgataatgac ggcctgatcc agaagatcaa gggggttggg
    13021 ccgaaaacag ctaaaaagat cgttattgaa ctgaaagacc gaataggaaa gtcaaccggc
    13081 acagaaacca caagttttgt tccgtctgac aataatatac gtgaagaggc gttatcagca
    13141 cttgctatgc tgggtttcag cagggctgct gctgataaag ccatccaggc agcgcaaaag
    13201 gccacgcctg aacttgcatc tgtagaagag ttggtaaaat cagcattgaa gaatttgtaa
    13261 aaggcgtgtt ttggcaggat tgagactctt ccctaaggta gcattggcag gtttgaccgg
    13321 tctggcggta actatttccg ctttccagca aacagctgca cctgccactt tgttcgtaag
    13381 atcccgccca aatatatacc tgccccttcc tgactccaca gataaagaca gcacagacac
    13441 cagcggtggc caggtgagtg attattttgc tgatgatcag aacgccttcg gtcttgatct
    13501 tccggcaggt ataacaccca cactggaata cgatcctgtt accggcgatt atatttattc
    13561 agaaaaagtt ggcgggcagt atttcaaaaa ccctcaatac atttccttcg aagattttct
    13621 gaaggctgaa caggaaaaat caataacaga ctattgggta gagcgttcca cggcccaaag
    13681 cattctgggt ggaagcggtg ccatcccgca gatctatact ggcagtgaac tgctggatcg
    13741 cctttttggt ggaagcaatg tggacattcg cccgcaggga aatatcgacc tgacatttgg
    13801 cggcagcttc cagaatgtgg gtaaccccat cctgaccgaa gatcaacgca gacagggagg
    13861 ctttgatttc aatatggaca tcaatgccaa catgctcgga cagatcggtg agaagatgaa
    13921 actcaccttc aactacaaca catctgctac attcgatttt gaaaaccagg taaaactgga
    13981 atataccggt tatgaagacg agatcatcaa aaagatcgaa gcaggtaacg taagtctgcc
    14041 actcacaact tcgcttattc ccggcacaca aagcctttgg ggtatcaaga cccaattgca
    14101 gtttggcaga ctgaccatga ccaacatcct ctctcagcaa cggtcacagt cgcaaagcat
    14161 acagattgaa ggaggcgctc agaaaagaga attcgaagtc ttcgccaatc agtatgatga
    14221 gaacaggcac tttttcctgg cgcattatat gcgtgaccac tatgaaccct ggttgagtac
    14281 attgcctttt gtcaactcgc cggtcaacat caccaggatg gaagtgtggg tcaccaacac
    14341 cacaggagcc acacagaaca cgcgtgatat tgtagcgttc acagacctgg gtgagaccga
    14401 ttctactttc tggtcagtag ccaacggcgg taccatcaat gtgctgagta cagacggtct
    14461 tccctccaat aatgccaacg atctgtatgg caaactggta aatgatgaca acgccagaaa
    14521 ccttaatacg gtcgtttctt ccctgtctga tcctgccggt cagtacaaga tgcagcctgt
    14581 tcgcgacttt gaaaaagtgc ggatgcggaa gctcaacacc agcgattatt cctataacaa
    14641 tcagctgggt tttatttctg tcaatacttc cttacagcca gacgatgtac tggctgttgc
    14701 ttttgaatat acctatctgg gccaggtgta tcaggtgggt gagtttacca ataacctgcc
    14761 tccgggtgta gatacctcca atgtcatgtt cctgaagatg ctgaagagca cttcagctca
    14821 ggtcaatatt ccgctgtggg acctgatgat gaagaacgtc tattctcttg gcgcttttca
    14881 ggtaagtccg caggattttc agctcgacat cctctaccag gatccgggtg gcggattcaa
    14941 aagatacatt ccggccaaca acaccaatgt gaatggccag cccatcatca ccctgctcaa
    15001 cctggacagg ctgaacacca atagtgaccc ttttccggat ggggtttttg actttgtgag
    15061 tggcattacc attaatacca acaacgggcg tatctacttc ccggtgctgg aaccattcgg
    15121 atcacacctg agatcagaac aggctttcgg aacagatcct gaagtggaac gctatgccta
    15181 cgatgaattg tactccacca cccgggtgct ggctgaacag tttccgcaat acaaccgctt
    15241 tgttataacc ggtagttaca aatcgagcgt cagttcagag atctacctcg gcgcatttaa
    15301 cctgcccaag ggatcggtca cagttacagc cggtggccag cagcttacgg agaatgtgga
    15361 ttataccatt gattattctc tgggaagact caagatcatc aatgaatcca tcctgaattc
    15421 cgggttgccc ataaatgtct cttttgaaaa caatgccttc tttggctttc aggtaaaatc
    15481 actcattggt tcacggttcg attactggat caatgacaat ttcactatgg gtgccacggt
    15541 attgcgatta tctgaaaggc cgtttaccca gaaggtgaac tttggagatg accctattca
    15601 gaataccatg ctgggctatg atgtcaatta tacggcagaa gcaccctgga tgaccagagc
    15661 attggacaaa cttccctttt acgataccaa agagatgtca tccatttcct ttaccggaga
    15721 ggtggccaga tttttacccg ggcatagtaa agccattggt aaagacgatg aaggggtcat
    15781 ttatattgat gactttgagg gaagcagaag tgcctatgac ctgaagtttc cgttttccag
    15841 ctgggtcata gcttccacac ctcagaatgc agtggatgcc aacggagacg tactctttcc
    15901 ggaagctacc ctgtttgaca gcctgatcta tggtatgaac cgtgctaaga ttgcatggta
    15961 caccatagac cccttgtttc tgcgggaaga caatgcggcc aggccggatc acctgactgc
    16021 cgatgatgtg tccaatcact acctcgtgga gatccagcaa caggagatat ttccacaagc
    16081 tgatattgcg gtaaccggac tgaacaacct cagcaccttt gacctggcct attatccgga
    16141 tgaacgcggt ccctataatt ttgatgtttc cggaactgcc tttagccagg gtgtcaatcc
    16201 tgatggatca ctcatcaacc cggcttccag atggggtggt atcatgcgaa gcctcgaaac
    16261 aaatgatttt gaagcagcca atattgagac catagagttc tgggtcatgg atccgtttga
    16321 taacctgggt ccattcggaa cacaggtgac agatggcggt gatctgtata tcaatattgg
    16381 taatgtatca gaagacatcc tcaaagacag ccggaccttc tttgagaatg gtcttccaaa
    16441 agatggctct accgctgatc ttgacgccac caactggggc cttgtgccac gcaccctgcc
    16501 ggtcaaccag gcatttgata acgacccgaa cagccgtgag aatcaggata aaggatatga
    16561 cggacagaac gatgcgcagg aggctggttt ttacgctgat tacctgaccc aactccaaac
    16621 cattcttaca cctgacgcac tgtctgttgc caccgccgat ccatcagcag ataactacca
    16681 ctattaccgc ggtggagatt atgacaacca ggaactgagc attttcgatc gttacaagag
    16741 atacaacaac ccgcagggaa actctccaac tacagaatct tctccggaaa attatcctac
    16801 ttctgcaacc aaccttcccg agtcagaaga cctcaaccgc gactataccc tgaacgaaac
    16861 cgaagcatac tttcagtacc gtattcccat cacccctgat atgatgacca ccaaccccta
    16921 tgtgacagat gtggtcaggg ctgagcatac ttttgaaaat ggagtggtgg acagtgtgac
    16981 ctggtatcag ctaaaagtcc ctatcaatca gtttgacgcc cgcataggta ccatccagga
    17041 ttttcgctct attcgtttca tcagaatgta tatgaccggt tttgaagaac cggtggtatt
    17101 gcgtttcgca cggctggaac tggtgcgtaa tcagtggaga aggtaccagt tcagcttact
    17161 ggagccaggc gagtatctgc ctgatgataa cagcggcgaa acagtattca atgtgagttc
    17221 tgtgagcatc gaagagaact ccggcagaca accgtttcct tatgtactgc caccggatgt
    17281 acagcgtgaa caaaccattg gcggtggtgg caacgtgagt actttccagc agaacgaaca
    17341 ggcactcagt atgcaggtct gtccgttgaa ggatggcgat tccagagcca tcttcaagtc
    17401 gctgcagctt gatcttcgcc ggtacggacg tttgatcatg aatgtgcatg gagaaccact
    17461 ggttggcagc cagaccactg atctgcagga tggtgatctg accgtttttg tgcgacttgg
    17521 ttctgacttc accaacaact attatgaata tgaggtgcct ctgaaggtga gtacacttcc
    17581 attgcctccc ggtactgccg atgatgatga cgttaaacgt gctgtctggg agacgctcat
    17641 agactttccg ctggactcac ttatatcagt caaatcagaa aggaacaacc tggaaatttc
    17701 accgctggtg ccttatgcag tgggtggtga agggcttccc acctatacag tgataggtaa
    17761 ccctgatctg ggttatgtgg aagaggtgat gataggggtg cgcaacccaa aaagcgtgag
    17821 tggttccggc gaagaatatt gtgcagaggt ctgggtcaat gaattgcgat tgagtgagat
    17881 cgatgagcag ggtggatggg ccgcactggc acgtgttgat gcccagctgg cagatctggg
    17941 caccatgacc ctgagtggta atatgcatac tgcaggtttc ggtacactgg aacagcagat
    18001 caacgaacga tatttcgaca acttttatca gtatgctgcc agcatgagcc tggaactggg
    18061 taaattcttc ccat
//
LOCUS       DAOMAX010000021        17977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_329_length_17977_cov_4.051724, whole genome shotgun sequence.
ACCESSION   DAOMAX010000021 DAOMAX010000000
VERSION     DAOMAX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17977
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_329_length_17977_cov_4.051724"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2566)
                     /locus_tag="PK742_02355"
     CDS             complement(<1..2566)
                     /locus_tag="PK742_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HQU75460.1"
                     /translation="MKQSILFLSLTLSAMLASAQVTIDNDLVLGGSENDVAYGLLSTS
                     DGNYLVSGYSRSTDFDVSANNGLYDYWIVKTSTAGAVIWETNLGGSGDDLARCAVEIS
                     GNYYVAGFTASADGDVSANNGGQDGWLVKLDGDGNLLWEKNYGGTGNDLILDMLAASD
                     GGVLMMGYTASADGDLSASYGNNDIWVIKTDADGNIDWSRNYGGSENDFGNAVVEIPG
                     GYIMTGATYSNDNDVSGNHADLSITSDSWVVRIDDSGDIVWQSCLGGVNNEIGEDLVI
                     TASNTIVVASSSKSDDGDVSGHYGELNKFDIWLCEVNNTGDVLQEQNFGGSRDDSPSA
                     LLIDDDGGFVVTGVSASDDFDLTGHYGAASAVDIWIMKTDDSFVLQWQKNIGGSQTDY
                     CEAFLPTGDTTYVLAAYTKSNDLDVSTHYGASGNNDIWMVYLVPGEEDTTSCDVAVTE
                     QPADILACPGADATFTVGGAGTVTSYVWIFTASGANFTTSEPSLTLEDVSTGFSSEFY
                     VILVGDCGNDTSELATFSVGELPAPDIFPVLPPSICEEPVITLSTPEVAGYFYQWYFN
                     GSLIPGATSFEYDATFAGEYYVVIDNGDGCSATSETVEITSSGPAALVTLDGPANICN
                     SGSVTMTTITGAGYLYQWYKDGSPIAGATTNTYTTSELGAYFVNINVDGCTSTSVTIN
                     VINETPESVTNALGDLDICESGSVSLTCTTSGVGYSFQWLKDDEAIPGATTITIFALD
                     TGSYTCVVSNTFGCSDTSEALLVYTSCPDTTVNPAVQDLSSLLGMQIYPNPAESVAQL
                     SVEVSAALTGSYQLQVMQTTGRVVEEMVIELGQGTRQINLETRNWPAGIYLVRLTHD"
     gene            complement(2608..4266)
                     /gene="recN"
                     /locus_tag="PK742_02360"
     CDS             complement(2608..4266)
                     /gene="recN"
                     /locus_tag="PK742_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQU75461.1"
                     /translation="MLLRLHISNYAIIDEAVIEPDKGLTVISGETGAGKSILLGALGL
                     VLGKRADTSVLRSNDRKCVVEATFLLKDLMLEDYFSENDLEYAEETIIRREIQPSGKS
                     RAFVNDSPVTLQILEPLASQLIQIHSQHETLDLSTSRFQFLMLDALAGHQELLKQYED
                     TFGNWKQVLAEIQEQENRLLRAKADEDYLQFQLSEMEAARLQDATPEDWEAELQQLEH
                     AEEIRQQLFTVSRLLQDGDPAAAQMLAEAVSALRGIAGYQDSIRQLEERLVSLRIELE
                     DIASEADKLGAAAQADPARAAMLQEKLSAIYQLYRKHQVQDLTALRALEEQFADQLNG
                     IQTGDTRLNALRHQAAMLQLRLEKDGAALTAGRKQAGIRLQKDLHTLLAKVGMKDAVL
                     EVTLPALPAPGPSGTSGVQFLFSANKGMAAREISKVASGGEMSRLMLCLKSLLAGTTA
                     LPTLIFDEIDTGVSGPIAYQVGEIMADLSTRHQVIAITHLPQIASRGSCHLLVAKKTS
                     AKIASTEIRPLDQDGRIAEIARMLSGEEAGQAALENARDLLRIR"
     gene            complement(4306..5205)
                     /locus_tag="PK742_02365"
     CDS             complement(4306..5205)
                     /locus_tag="PK742_02365"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HQU75462.1"
                     /translation="MRNHLLLLLLLFTALAAPAQELNCGVSINTQQLKTTDPKVFATL
                     ETSIKEFMNTRKWTNDVFLPEERIDCEFVISITEEVSSDRFKAQASIISRRPVFGTDY
                     NSVMLNTVDKDWEFQYVEYQPLEFNEAVFLNNLTSMLAYYAYIIIGMDYDSFGKKGGD
                     IYYQKAYTIVSQASNREESGWKSYDGTRNRYWLVNNLTDPRFSGMRDVYYNYHRLALD
                     RMYEDQSKPISVITKALQTMDNMNRAQPNSMVIQLFFSAKSSEILGLYGKALPAEKSK
                     AVTYLMRLDPLNAEKYQGLLSGN"
     gene            complement(5198..6409)
                     /gene="coaBC"
                     /locus_tag="PK742_02370"
     CDS             complement(5198..6409)
                     /gene="coaBC"
                     /locus_tag="PK742_02370"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQU75463.1"
                     /translation="MRLSGTRILLGVTGGIAAYKSAYLVRLLVNEGAAVRVVMTPGAR
                     AFIGPVTLSTLSKNPVSTDFFDELSGEWSNHVELGLWPHVMLFAPLTANTLSKMAHGT
                     SDNLLLATYLSARCPVLFAPAMDLDMWTHPATAANLETLLKRGHQLIDPGTGELASGL
                     QGKGRMAEPEVILEKIIERFGREHRPLSGRKILITAGPTYEAIDPVRFIGNHSTGKMG
                     FALAQAAHDQGAEVTVVHGPVSVPLQDIPFQLVAVQSADEMLHACQQIFSQCDVLIAA
                     AAVADYRPEHTASEKIKKEDQADMTIRLVRNPDILATLSAQKHTGQLIGGFALESSSG
                     EDEARRKLQQKDLDFIVLNSLQDAGAGFAHHTNKISILDRRNKFISFELKEKTAVAED
                     IVQHLIGLLHA"
     gene            complement(6412..6750)
                     /locus_tag="PK742_02375"
     CDS             complement(6412..6750)
                     /locus_tag="PK742_02375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQU75464.1"
                     /translation="MNSPKKFNQHLNPSVEVQDMGKIEKGSGNVYRSIYIIGKRSNQI
                     AAQIREELHHKLEEFATHQDNLEEVFENREQIEISKYYEKLPKSTILSLDEFKNGKIY
                     TRRRDEENAE"
     gene            complement(6743..7567)
                     /gene="bamD"
                     /locus_tag="PK742_02380"
     CDS             complement(6743..7567)
                     /gene="bamD"
                     /locus_tag="PK742_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQU75465.1"
                     /translation="MKKGVLFFSLVIWLLSGCSDYEKLLKSDDFDLKYDRAKAYYNEG
                     DYARALPLLDQMLTVRMGTADEEEIRYYIAYCYYGQSQYLISSTLFKNFFISFPRSYR
                     AEECLYQSAYSLYQASPPYYLDQSQTYKALSEFQYFADTYKKSDRVTDANRIMDELRA
                     KLELKSYRSAQLYFDTENYQAAAVTWENLLVDFPETDEAEDIGQKIARSWFNYATQSV
                     PCKKPERFDKAVESCDIFREKFPESTLLPAVNALRERSLEQKERALTEKENYDCDE"
     gene            7704..8945
                     /locus_tag="PK742_02385"
     CDS             7704..8945
                     /locus_tag="PK742_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HQU75466.1"
                     /translation="MDIFDKLRNDPGPLGKWSDVGHGYMMFPKLEGDIAPRMMFRGKE
                     RIVWSLNNYLGLANDPEVRKTDEEAAGAFGMAYPMGSRMMSGNSDHHEQLESELSAFV
                     QKEDTFLLNYGYQGIMSAIDCICGRHDVIVYDAESHACIIDGVRLHQGKRFVFRHNDM
                     ENFETQMRRATELVEGTPGGILVITEGVFGMGGDQGKLKEIVEFKKKFDFRLLVDDAH
                     GFGTMGPTGAGCGEHQGCQDGIDLYFSTFAKSMASIGAFISGEKAIIKFIRYNSRSQI
                     FAKSLPMPIVLGNLKRLELLRNRPELRQKLWDNVEKLQSGFRERGFNIGETNSPVTPV
                     FLKVSIPETINLVVDLRENHGIFCSAVTYPVIPKGQLMLRIIPTAAHSDEDIRLTLDA
                     FSAIRENVENGAYLKEMPVNV"
     gene            9004..9177
                     /locus_tag="PK742_02390"
     CDS             9004..9177
                     /locus_tag="PK742_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75467.1"
                     /translation="MEEKLAQIKSIISSAEEDMMKFLDKGNKTAGTRLRKELQQIREI
                     AQDIRKEVLERRK"
     gene            complement(9181..10083)
                     /gene="dapA"
                     /locus_tag="PK742_02395"
     CDS             complement(9181..10083)
                     /gene="dapA"
                     /locus_tag="PK742_02395"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQU75468.1"
                     /translation="MHQYTAQQIDRFKGTGVALVTPFNQDGSIDFASLEKLIEHVIAG
                     GVEYLVSMGTTGESVTLTEQERFDIIRFTAKINRKRAGLVAGFGGNNTAAVTASIRKF
                     DFTDVDGILSNSPYYNKPSQEGIYQHYMAIDAVSPVPVILYNVPGRTSSNITALTTMR
                     LANDARHVVGIKEASGNFSQIMELCSRLPESFLVLSGDDAITLPMISTGAHGVISVIA
                     NAYPAIFSNMVRFALDDKFAKARPLHNSLLEITDLIFREGSPAGVKHVLSEMEICKTH
                     LRLPLTNISENLQMLLGDAMKEIG"
     gene            complement(10055..10627)
                     /locus_tag="PK742_02400"
     CDS             complement(10055..10627)
                     /locus_tag="PK742_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75469.1"
                     /translation="MKLNILPGSLARRAAGKYIRKKSYAGSRNRKITNWAEVKSIAFL
                     VDGHDPAALRMLLNQLHAYAQKGKKVHIFGYVKKLPPFEEDGIYWFTNKDLNWAGLPK
                     PSVLKALNIQTYDLLINTAEIKVSTLHYLSALSHATLRIGPYAEEWAECYDFMIIQDP
                     GETVPELLKKAEYYLSTFNKQYAPVHGTTD"
     gene            complement(10624..11583)
                     /locus_tag="PK742_02405"
     CDS             complement(10624..11583)
                     /locus_tag="PK742_02405"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQU75470.1"
                     /translation="MIFLDFEQPIADLYEELEKLREVGQKQGIDISQPEKELQDKITD
                     AKTRIYSELNPWQRVQLSRHPDRPYTLFYIEQMCDAFTELHGDRNVGDDKAIVGGFGK
                     LGDETVMIIGHQKGVNTKMRQYRNFGMANPEGYRKALRLMKLAEKFNRPVITLIDTPG
                     AFPGLEAEERGQAEAIARNLFEMTLLKVPVICIVIGEGASGGALGIGIGDRVLMLENT
                     WYSVISPESCSSILWRSWDFKEQAAEALKLTSEDNLRNHLIDGIIREPLGGAHSQPEE
                     MAAIMKREILQMLQELKPVDPEQRIDDRVRKFSAMGVYAETTA"
     gene            11711..12421
                     /locus_tag="PK742_02410"
     CDS             11711..12421
                     /locus_tag="PK742_02410"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HQU75471.1"
                     /translation="MKNTLLMLLFATTISVASAYPGSRSSLEIVSYSGPISVSVDRQY
                     FGQPTDHFAVDNIKPGNRYIEIFGIRFHHGHAERVKLFAGRVFISNDSRISAVFDERR
                     GFYIAETRPYGTYGYGGYQGYDTYGYGQDQGYYGYASPVPMSSGQFSGYLDVVDDQWT
                     ESNRLQVALQGLTYNWLTTLQVKAVMETFWTESARLEFAKAAYSRVVDPQIYYEVNEV
                     FWSQSSVQELSRYILQGR"
     gene            complement(12494..13915)
                     /locus_tag="PK742_02415"
     CDS             complement(12494..13915)
                     /locus_tag="PK742_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU75472.1"
                     /translation="MYIEQIYTGCLAHGAYYIESNGEAAIIDPLRETAPYIQMAEDRK
                     ARIKYVLETHFHADFVSGHVDLAQKTGATIVYGPTTMKTGFDMLVATDGQEFELGNIR
                     IRLLHTPGHTMESSCFLLIDEEGREKALFTGDTLFIGDVGRPDLAQKVIAELTQEKLA
                     SMLYDSLRNKIMPLTDDLIIYPAHGAGSACGKNMSKETSDTLGHQKATNYALRADMTR
                     EEFMQELLTGLTPPPGYFPKNVLMNIGGYDQFDDVMNRGSQPLSPAAFEAAANETGAL
                     ILDTREQQSFASGFIPNAINISLDGNFAVWVGTLVPDIKQEILLIAEPGREKEAITRM
                     ARVGYDHCIGYLDGGMSSWIHAGKETDTISSLTPQELAQRWPDNDAVLLDVRKKSEFD
                     SEHVEGAENVPLDYINERMQEVDPGKTYYVQCLSGYRSMTFVSILKARGYEKLINVKG
                     GFDAIRKSELFPLTDYVCPTTLL"
     gene            14168..14806
                     /locus_tag="PK742_02420"
     CDS             14168..14806
                     /locus_tag="PK742_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQU75473.1"
                     /translation="MQKFIPIFPLGMVAYPGEQLNLHIFEERYKQLINECVQENKSFG
                     IPPVEGKEVLLYGTELLVTEVVKKYDNGELDVRVEGTGVFRILEVIHEIPEKLYMGAI
                     VTVLDNINDRHMPTRERLRDAVNELFALLDIDKRILPEEQAYYSYALAHLVGFDLLQE
                     FELLKHQSESVRQRIILEHVRNLLPSVRSVHDIRERARLNGQFRMINPPDGI"
     gene            complement(14856..>17977)
                     /locus_tag="PK742_02425"
     CDS             complement(14856..>17977)
                     /locus_tag="PK742_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQU75474.1"
                     /translation="KRKNRLWVNDGNMHFTEEAAKYGLDDDSYSSHADFFDYDNDGDL
                     DMYLVTHPVDFIDKNKTKYFQKIEKGVNLSDKFYVNNGDGTYTEKHKEVGINNHGYGL
                     SGTVGDLNSDGYLDIFVSNDYAMYDFIYINNGDGTFRDESLNAVKKTSINSMGSDVND
                     YNNDGYPDIIVADMDMEDNYTYKTFMLSSQVEVMRILINAGYGYQNRSNSLQLNNGDG
                     TFGEISRTAGVATTDWSWSTLFADFDNDGWKDLFIANGFLRDFHVDESETYHKLRRAV
                     RIEDSSVYYEVREKLPHYVLSHPNFIFRNNGDLTFTDSRDEWGIYYPSITYGAGYADL
                     DGDGDLDIIGSNVNETPWIYRNNSEKFDKQNYINFQFEGYGANAMGLGTKVRVWVDGQ
                     QQFIQHSNVRGYITTMENDVHFGLGSHEKVDVVEVEWLDGAKQVLRDVTANQTITLSH
                     KDAQKNIRFDDHGRTDQLFVNMTQDLGTDFKHTEDDYDDYLREYLLPHKMSSLAPGIA
                     VADVNGDGRQDFYIGGAIGQSGAIYTQRPDGSFERSDFQTKQPDDLELEDAGALFFDA
                     DSDGDMDLYIATGGNEYPDMDKRYADRLYINDGKGSFTRNAEAIPATYSSSSVITAAD
                     FDKDGDMDLFVGGRQVPGRYLASTSSYILQNNNGKFEDVSDKLAPALKEIGMVTSALW
                     TDFNNDNEQDLIITGDWMPITFLQNKGGKFADVTAQTGMVNTSGWWQSIAAADFDNDG
                     DMDYVVGNFGTNRRYRNTRSEADGRALPLEAFLADFDSNGKEDFIMAYYQHDQLYPVK
                     TRERLIEQMPSIGDKFPTWDAFGKATVTEILGKEAIEKSVHKDAYIFHSSVLMNEGNG
                     KFKMSYLPNEAQISVLFGMVVDDFNNDGYMDILAQGNFYNTEIEITRHDAGTGILLLN
                     NGKGKFSPERSISSGFRNDGDAKGMATILVGANNQPVYLLGNSDGNMLSYGLTSVVKT
                     IPLNESDAFAMVTLKNGEVRRQELQAGSGYLSQSAKFIRVSDLVSSVEVTSYNGQTRT
                     VYPGQTASK"
BASE COUNT         4365 a         4348 c         4395 g         4869 t
ORIGIN      
        1 catcgtgtgt aagtctaacc aggtagatac ctgccggcca gttccttgtt tccaggttga
       61 tctggcgtgt tccctgacct aactcaatga ccatttcttc caccactctg ccggtggttt
      121 gcatgacctg cagctggtag ctgccagtga gtgcagcaga aacttctact gacagctggg
      181 caacagattc tgccggattg gggtagatct gcataccaag cagactgctc agatcctgca
      241 ccgccggatt gacagtagta tctgggcaag aggtatatac aaggagggcc tctgatgtat
      301 cgctgcagcc gaaagtattg ctgacaacac aggtatagga tcctgtgtca agggcaaaga
      361 tggtaatggt tgtggcaccg ggtatagctt catcatcttt caaccactga aaactgtaac
      421 ctacgccgct ggtggtgcag gtcagactaa cactgccaga ctcgcagatg tccagatcac
      481 caagcgcatt ggtaacactt tcaggtgttt cattgatcac attgatggta acagaagtgc
      541 tggtgcaacc atccacattg atattgacaa agtatgcgcc cagttcactg gtggtatagg
      601 tattggtagt agcacctgca atcgggctgc catctttata ccactggtag aggtatcctg
      661 ctccagtaat ggtggtcatg gtaacgctgc ctgaattaca gatgttggca ggaccgtcca
      721 gagtgaccag agcggccggg ccggaactgg tgatctcaac ggtttcagaa gtggcagaac
      781 agccatcacc gttatcgatt acaacataat attcgccggc aaaggtggcg tcgtattcaa
      841 agctggtagc tccgggaatc agagatccgt tgaaatacca ctgatagaaa tatcctgcca
      901 cttccggggt tgacaaggtg attacaggtt cttcacagat agagggaggc aacacgggga
      961 agatatcagg tgcaggcaat tcacctactg aaaaggttgc cagttctgaa gtatcgtttc
     1021 cgcaatcgcc caccaggatc acatagaatt cgctgctgaa gccggttgat acatcttcca
     1081 gtgtcagtga aggttcgctg gtggtgaagt tggcgccgct ggcagtgaag atccagacgt
     1141 aactggtaac ggtaccggca ccgccaactg tgaaggtggc atctgccccc ggacaagcca
     1201 gtatgtctgc tggttgttcg gtaacagcca catcgcagga agtagtgtct tcttcaccgg
     1261 gcaccaggta aaccatccag atatcattgt taccgctggc accgtaatgt gtacttacat
     1321 ccagatcatt ggacttggtg taggctgcca gtacataggt ggtatcgcct gtaggcagga
     1381 aggcctcaca ataatcagtc tgagatccgc caatattttt ttgccattgc agaacaaaac
     1441 tatcatcggt cttcatgatc cagatatcaa ctgcactggc tgctccgtaa tgaccagtca
     1501 ggtcaaaatc atctgaagca gaaacacctg ttactacaaa gcctccgtcg tcatcaataa
     1561 ggagtgcgct ggggctgtca tcacggctgc caccaaaatt ttgttcctgc agtacatcgc
     1621 cggtgttgtt gacttcgcag agccagatat cgaatttatt cagttcgcca tagtgtcctg
     1681 atacatctcc gtcatctgac ttactggaag aggctaccac aatagtattg gatgcagtaa
     1741 tgaccagatc ttcgccaatt tcattgttca caccaccaag gcatgattgc catactatat
     1801 ctccgctgtc atctatcctg accacccagg aatctgaggt aatagacaga tctgcgtggt
     1861 tgccactcac atcgttgtca ttggaatagg tagcaccggt catgatgtat ccgccgggga
     1921 tctctactac agcattgccg aaatcgtttt cagaaccacc ataattcctg ctccagtcaa
     1981 tattaccatc ggcatcagtt ttgatcaccc agatatcatt attgccatag ctggcactga
     2041 ggtctccatc ggcagaagca gtgtagccca tcatcagtac gccaccatcg gaggcagcca
     2101 gcatatccag gatcaggtca tttcctgttc ctccgtagtt cttttcccac aacaggttgc
     2161 catcaccgtc cagcttcacc agccagccat cctgtccacc gttgttggca gaaacatcac
     2221 cgtctgcgga agcagtaaaa ccggcaacgt aatagtttcc gcttatttct accgcacatc
     2281 ttgccaggtc atcgccgctg cctcccagat tggtttccca gatcactgca ccggcagtgc
     2341 ttgtcttgac gatccaatag tcatacagac cgttgttggc agacacatca aagtctgttg
     2401 accgggaata accggatacc agatagttgc catcagaagt gctcaggagg ccataagcca
     2461 catcattttc tgatccgccc agcaccaggt cgttgtcaat ggtaacctgt gcagaggcca
     2521 gcatggcact aagggttaag gaaaggaata gtatgctttg tttcatttca ccgtggtttg
     2581 gtgcaaaggt agtaatagaa aaagggatca ccttattctg agcagatctc tggcattttc
     2641 gagggcagcc tgaccggctt cttcgccact gagcatgcgg gctatttcag ctattcgtcc
     2701 gtcctgatcc agcggtctga tctcggtgct ggcaattttg gcggaggttt ttttggcaac
     2761 cagcaaatga caggatcctc tggaagcgat ctgcggaagg tgggtaatag caatcacctg
     2821 atgccgcgtt gagaggtcgg ccatgatctc accaacctga taggcaatag gccctgaaac
     2881 acctgtgtct atttcgtcaa agatgagcgt aggcagggca gtagtacccg ccagcagaga
     2941 tttcaggcag agcatcagcc ggctcatttc tcctcctgat gctaccttgc tgatctcacg
     3001 ggctgccatt cctttattgg cagaaaacag gaactgcaca ccgcttgttc cggaaggacc
     3061 cggggccggt aaggccggta gcgtaacctc cagtaccgca tccttcatac caacttttgc
     3121 cagcagtgta tgcaggtctt tttgaagacg gatgccagcc tgctttcttc ctgccgtaag
     3181 tgctgcgcca tctttttcca gcctgagctg cagcatggca gcctgatggc gcagggcgtt
     3241 aagccgggta tcgccggtct gaataccgtt cagctgatct gcaaactgtt cttccagtgc
     3301 acgcagtgcc gtcagatcct gtacctgatg ctttctgtat aattgataaa tggcagacag
     3361 tttttcctgc agcatcgcag cccttgccgg gtctgcctgg gcagcagcgc ccagcttgtc
     3421 tgcctctgag gcaatgtctt ccagttcaat acgaagtgat acaagacgtt cttccagttg
     3481 cctgatgctg tcctgataac cggcaatgcc acgcaatgca gagaccgcct cagccagcat
     3541 ttgtgcggct gcaggatctc catcctgcag caaacgagac acggtgaata actgctgcct
     3601 gatctcttcg gcatgttcaa gctgttgcaa ttcagcttcc cagtcttctg gggtagcatc
     3661 ctgcagcctc gcagcttcca tttcgctgag ctgaaattga aggtaatctt catcagcttt
     3721 ggcccgaagc agcctgtttt cctgctcctg tatttcagca agcacctgtt tccagttgcc
     3781 aaaagtatct tcatattgtt tcagcaactc ctggtggccg gcaagtgcat ccagcatcag
     3841 gaactgaaag cgggaagtgc tgaggtcaag ggtttcatgt tgagaatgta tctgtatcaa
     3901 ctgagaagcc aggggctcca gtatctgcag ggtaacgggg ctgtcattga caaaggctct
     3961 ggatttaccg gaaggctgga tctcccgtct gataatggtt tcttcagcat attcgaggtc
     4021 attctcagag aaataatctt ccagcatcag atccttcaac aggaaagtag cttctaccac
     4081 acatttccgg tcattgctgc gcagtacact ggtatcggcc cttttgccca gtaccaggcc
     4141 cagagccccc agcaggatag attttcctgc accagtttca ccactgatta ccgtaagccc
     4201 tttgtcgggc tctatgacag cctcatcaat gatggcgtag ttggatatgt gcaaacgaag
     4261 cagcatggca gaatatgccg gtaaaaatag ccagaaatcc ggggcttaat tgcctgataa
     4321 cagcccctga tacttttcag cattcagagg gtccaggcgc atgagatagg ttactgcctt
     4381 gcttttttct gccggcagtg ccttgccata caatccgaga atttcagatg attttgctga
     4441 aaagaaaagc tgtatgacca tgctgttggg ctgggcacga ttcatattat ccatggtttg
     4501 caaggccttg gtaataactg aaataggttt ggactggtct tcatacatcc tgtccagggc
     4561 cagtctgtgg tagttgtaat aaacatcacg catacctgaa aaccgggggt ctgtcaggtt
     4621 gttgaccagc cagtagcggt tcctggtgcc atcataagat ttccatccgc tttcttcacg
     4681 gttggacgcc tggcttacaa tagtatatgc tttttgataa tatatatcgc cgcctttttt
     4741 gccaaaggaa tcataatcca tacctattat gatataggca taataggcca gcatagaggt
     4801 aaggttgttg agaaatacgg cttcattgaa ttcaagtggc tggtattcta catattgaaa
     4861 ctcccagtcc ttgtcaaccg tattcagcat gacggaatta tagtcagtgc cgaataccgg
     4921 acggcgggaa atgatactgg cctgtgcctt gaatctgtct gagcttactt cttccgtaat
     4981 ggagatcacg aattcacagt caattctttc ttcgggcagg aaaacatcat tggtccactt
     5041 acgggtgttc atgaactctt tgatactggt ttcaagggta gcaaacactt tggggtcagt
     5101 ggttttcagc tgctgggtgt tgatgcttac accgcagttg agctcctggg caggagcagc
     5161 gagagcagtg aacagcagga ggagaagcaa caaatgatta cgcatgcagt agtccgatta
     5221 ggtgttgcac gatgtcttcg gcaacagcgg tcttttcctt taactcaaaa ctaataaact
     5281 tattacggcg atccaggata ctgatcttat tcgtatggtg ggcgaaacct gcgccggcat
     5341 cctgtaagga attcagaacg atgaaatcca ggtctttttg ttgcagcttt ctccgtgctt
     5401 catcttcacc ggaactgctt tcaagggcga acccgcctat cagttgaccg gtatgttttt
     5461 gggcggataa tgtagccagt atgtccggat tgcgaaccag ccggatggtc atatctgcct
     5521 ggtcttcttt tttgatcttt tcagatgccg tatgttccgg ccgatagtct gccacagctg
     5581 cagcggcaat aagtacatcg cactgactga atatctgttg acaggcgtgt aacatctcat
     5641 ctgctgactg aacagctacg agttgaaatg gaatgtcctg taatggcacc gaaacaggtc
     5701 cgtgcaccac agttacctct gcaccctgat catgagcggc ctgagccagg gcaaacccca
     5761 tcttacccgt tgaatggttg cctatgaaac gaacaggatc gatcgcttca taggtgggcc
     5821 cggctgtgat caggatcttc ctgccactca gcggtctgtg ttcacggcca aaacgttcaa
     5881 tgatcttttc cagtatcact tctggttctg ccatacgccc ttttccctgc aggccactgg
     5941 ccagttcgcc ggtgcccggg tcaatgagct gatggcctct cttcagcaat gtctccaggt
     6001 tggctgcagt tgccggatgt gtccacatat ccagatccat ggcgggagca aacaggaccg
     6061 gacatctggc agacagatag gtggccagca gcaggttatc gctggttccg tgggccattt
     6121 tggacagggt attggcggtg aggggagcaa aaagcatcac atgaggccat aaacccagct
     6181 ccacatgatt agaccattcg cctgacagct catcaaaaaa atctgtagat acgggattct
     6241 tagacagtgt tgacagggtg accgggccta tgaaggcacg ggctccgggt gtcatgacca
     6301 cacgcactgc ggcaccttca ttcaccagca ggcgaacaag ataggcgctt ttgtaggcgg
     6361 caatgcctcc ggtcacgccc agcaggatcc gtgtaccgga aagtcgcatg attactcagc
     6421 gttttcttcg tcgcggcgac gggtatagat cttgccattc ttgaattcat ccagtgaaag
     6481 gatggttgac tttggcaatt tctcatagta tttgctgatc tcgatctgct cgcggttttc
     6541 aaatacctct tccagattat cctggtgggt agcgaattct tcgagcttat ggtgcagctc
     6601 ctcacggatc tgcgctgcga tctggttgct ccgcttgcct atgatgtaga tggatcggta
     6661 cacgttgccg gaacctttct cgatcttacc catatcctgc acttctacag agggattcaa
     6721 atgctgattg aacttcttag gactattcat cacaatcgta gttttctttt tcggtcaatg
     6781 cccgttcttt ttgttccaga ctccgttccc gaagggcatt tacggcaggt aacagagtgc
     6841 tttcgggaaa tttctcccga aagatatcac aactttcaac agccttgtca aagcgctccg
     6901 gttttttaca tggaacactc tgtgtagcat aattgaacca ggaacgggct attttctgcc
     6961 cgatgtcttc agcttcgtct gtttcaggga aatcgaccag caggttttcc caggtaacag
     7021 cagctgcctg atagttttct gtgtcaaagt aaagctgggc gcttcggtaa gacttcagct
     7081 ccagtttggc ccgaagttca tccatgatgc ggttggcatc agttacccgg tcgctctttt
     7141 tatacgtgtc agcaaaatac tggaattctg acagagcctt gtaggtctgg ctttgatcca
     7201 ggtagtaggg tggtgaagcc tgatacaggc tgtaggcact ctgataaaga cattcttcag
     7261 ccctgtagga acgggggaag gaaataaaga aatttttgaa cagggtactg gagatcaggt
     7321 actggctttg accataataa cagtaagcaa tatagtagcg gatctcctct tcatctgccg
     7381 tacccatgcg cacggtcagc atctgatcaa gcaggggcag ggcacgtgca tagtctcctt
     7441 cgttgtaata ggctttagcc cggtcatatt tcagatcgaa atcatcgctc ttcagcagct
     7501 tttcataatc agaacaaccg cttaacagcc agatcaccaa agagaagaag agtacacctt
     7561 ttttcatgga ctaaaagcct gcaaaattag aaataatcca ccaataacgg ataattgaac
     7621 acaacggtat ctgtgccggc ggttatttta ggaagtatgg gaatcggcgt atctttgcgc
     7681 caggcaaacc attacaagaa cctatggata tttttgataa actgcgcaac gatccggggc
     7741 cactgggtaa gtggtctgac gtgggtcacg gctatatgat gttccccaaa ctggaagggg
     7801 atattgctcc acgcatgatg ttccgcggaa aggaacggat tgtctggagc ctgaataact
     7861 acctgggcct ggccaatgac ccggaggtca gaaagaccga tgaagaagcc gcaggggcat
     7921 ttgggatggc ttaccccatg ggttcccgca tgatgagcgg taattctgac caccatgaac
     7981 agctggaatc agaactgagt gcttttgtgc agaaggaaga cactttcctg cttaactacg
     8041 gttaccaggg tatcatgagt gccattgact gtatatgtgg tcgtcatgat gtcattgtat
     8101 atgacgcaga aagccatgcc tgcatcattg acggagtgcg attgcaccag ggtaagcgct
     8161 ttgttttccg ccataatgat atggagaatt tcgagaccca gatgcgcagg gctaccgagc
     8221 ttgtagaagg tactccgggg ggcattctgg tcatcacgga aggcgttttt ggtatgggcg
     8281 gcgatcaggg caagctgaaa gagatcgtgg aattcaagaa aaagtttgat ttccgtttgc
     8341 tggttgatga cgcacatggt tttggtacca tgggccccac cggtgcgggt tgcggcgaac
     8401 atcagggttg tcaggatgga atagacctgt atttctccac ttttgccaaa tctatggctt
     8461 ctattggggc ctttatcagt ggtgaaaagg ccatcatcaa attcattcga tacaattcac
     8521 gctctcagat ctttgccaaa tcacttccta tgccaatagt gctgggtaat ctgaaaagac
     8581 tggagctgct acgcaacaga cccgagctgc gtcagaaatt atgggataat gtagaaaagc
     8641 tacagtcagg tttccgtgag cgaggattca acattggcga gaccaatagc cctgtaacac
     8701 ctgttttcct gaaggtaagc atacccgaaa ccattaatct ggtagtagat ctgcgtgaga
     8761 atcatggcat tttctgctct gcggtgacct atccggtcat tcccaaaggc cagctgatgc
     8821 tgcgtatcat ccccactgca gcccattcag atgaagatat caggctcacc ctggatgcat
     8881 tcagcgctat ccgtgagaat gtagaaaatg gtgcgtattt gaaagaaatg cctgtaaatg
     8941 tgtaatttca tccttgccac ggttggaaca cttatttact gacacccaaa acaaaccatc
     9001 cagatggaag aaaaactcgc tcaaatcaag tcgatcatct cttctgcaga ggaagacatg
     9061 atgaagtttc ttgacaaagg caacaagacc gccggtaccc gcctgagaaa agaacttcag
     9121 caaattcgcg agatcgccca ggatatccgt aaggaagttc tggagcgcag gaagtgatct
     9181 ttacccgatc tctttcatcg catcacctaa cagcatctga aggttttcac tgatattggt
     9241 cagtggaagc ctcagatgtg ttttacagat ctccatttca ctcaatacat gcttgacacc
     9301 tgccgggctt ccttcccgga agatcaggtc tgttatttcc agcaaactat tgtgcagagg
     9361 acgggctttt gcaaatttat cgtccagggc aaacctgacc atattactga aaatggcagg
     9421 gtaggcattg gctataacac tgatgacacc atgtgctccg gtactgatca tcggaagggt
     9481 gatggcatca tcgcctgata atacaagaaa agattcaggc agtctggaac acaattccat
     9541 gatctgagag aaatttcctg aggcttcctt gatcccaacc acatgcctgg catcattggc
     9601 cagtcgcatg gtggtcagtg ctgtgatatt gctgctggtt cgcccgggta cattgtacag
     9661 gattaccgga accggcgata ccgcatcaat ggccatatag tgctggtaaa taccttcctg
     9721 actgggcttg ttgtagtaag gtgaattgga taaaatgccg tccacatcag tgaaatcaaa
     9781 tttccggatg gaggcagtaa cagctgcggt attattgcca ccgaatccgg ccacaagacc
     9841 ggcacgtttg cgattgatct tggcggtaaa gcgtatgatg tcaaatcgtt cctgctcggt
     9901 cagggtaact gattcgccgg tggttcccat gcttaccaga tattctacac ctcctgctat
     9961 cacgtgttcg atcagttttt ccaacgaggc aaaatctatg cttccgtcct gattgaatgg
    10021 agttaccagg gcaacccctg tacctttgaa tctgtcaatc tgttgtgccg tgtactggtg
    10081 catattgttt attgaaggtg ctcagataat attctgcttt tttcaggagt tccggtactg
    10141 tttctccagg atcctgtatg atcataaaat cataacattc tgcccattct tcagcataag
    10201 gccctatgcg caaggtggca tgcgacagtg cagacaggta atgcagggtg gatactttta
    10261 tttctgccgt gttgatgagc agatcatagg tttggatgtt aagagctttc aagaccgatg
    10321 gcttgggaag accggcccag ttaaggtctt tattggtaaa ccagtagata ccgtcttctt
    10381 caaagggcgg taactttttg acgtatccga aaatatgtac tttctttcct ttctgggcat
    10441 aggcatgcag ctggttcagc agcatacgca gtgcggcagg atcgtgcccg tcaaccagaa
    10501 aagcaatgct tttaacttcg gcccagttcg tgatctttct gtttcggctt ccggcataag
    10561 actttttgcg aatgtacttg cccgcagccc gtctggccag tgaaccggga agaatattca
    10621 gtttcatgca gtggtttctg cgtaaacacc catagcagag aatttcctga cccggtcatc
    10681 tatgcgttgt tccggatcaa ccggtttcag ctcctgcaac atttgaagga tctcacgctt
    10741 catgatagcg gccatttctt ccggctggct gtgagcacca cccagcggct cacgtataat
    10801 gccgtcaatg agatgatttc gaaggttgtc ttctgaagtg agtttgaggg cttcagcagc
    10861 ttgttctttg aagtcccagc tgcgccagag aatggaagaa caagactccg gcgaaatgac
    10921 tgaataccag gtgttctcca gcatcagtac cctgtctcct ataccaattc ccaatgcacc
    10981 gccggaggca ccttcgccaa tgacgataca aataacaggt actttcagca aggtcatttc
    11041 aaacaggttc cgggcaatgg cttcggcctg cccgcgttct tcagcttcca gacccggaaa
    11101 ggcgcccgga gtatcaatca gtgtgatgac aggcctgttg aacttctctg ccagtttcat
    11161 cagccgaagc gctttacggt acccttccgg atttgccatg ccgaagttcc ggtactgccg
    11221 catcttggtg ttgactcctt tctgatgacc aatgatcata actgtttcat cgcccagttt
    11281 cccgaagcca cccacaatgg ctttgtcatc acccacattt ctgtcgccgt gcaactcagt
    11341 gaatgcatca cacatttgtt caatatagaa caaggtgtac ggacgatccg gatgcctgga
    11401 cagctgtaca cgctgccacg gattgagctc tgagtagatc ctggtcttgg catctgtgat
    11461 cttatcctgc agttcctttt caggctgaga gatatctatg ccctgcttct gacctacttc
    11521 acgcaacttc tccagctctt cgtacaggtc ggcgatgggt tgttcaaaat ccaggaatat
    11581 catgtgtcaa aattagagaa aacttattac tcatcttacc tgacaggcag gcactgatgg
    11641 ccgttctgta cctgttgcag gaaagttggc acaataatgg aataaccaac attaaaacaa
    11701 ggaggttgtt atgaaaaaca cattactcat gctgctgttc gccactacta tttcggttgc
    11761 gtcagcctat ccgggtagtc gttccagcct ggagatcgtt tcgtattcag gtcctatctc
    11821 agtaagtgtg gacaggcagt atttcggaca acccactgac cattttgcgg ttgacaacat
    11881 caagcccgga aaccgctata tcgaaatttt cggaatacgg tttcaccacg ggcatgcaga
    11941 gcgggtgaag ttgtttgcgg gtcgggtgtt catttcgaat gacagtcgca tcagtgctgt
    12001 atttgatgag cgcagagggt tctatattgc tgagacccgt ccgtacggca cctacggcta
    12061 tggtggatat cagggttacg atacttacgg ttacgggcag gatcagggtt actacggata
    12121 tgcgtcaccc gttccgatgt catccggtca gttcagcggt tatctggacg tggtggatga
    12181 tcagtggact gaaagcaacc ggcttcaggt ggcattgcag ggattgactt acaactggct
    12241 gaccaccctt caggtgaaag cggtcatgga aacattctgg acagaaagcg ccagactgga
    12301 atttgccaaa gcagcctact ccagggttgt ggatcctcag atctattatg aggttaacga
    12361 agtattctgg tcacagtcca gcgtacagga actgagcaga tacatcctgc agggaaggta
    12421 acctcgcaac aggatcgttt gcatagaaag ggggcacccg ccgggcgccc cctttttcat
    12481 atatccccgc taattaaaga agtgtggtag ggcagacgta gtcagtaaga ggaaaaagtt
    12541 cgctctttct gatggcatca aaaccgccct tcacattgat aagtttttca taaccccttg
    12601 ctttcaggat ggagacaaag gtcatggaac gatagccgga aagacactgt acatagtagg
    12661 tcttgccggg atctacttct tgcatccttt cattgatata gtccaggggt acattttcag
    12721 cgccttccac atgttcactg tcaaactcac tttttttgcg cacatccagc agaacggcat
    12781 cgttatccgg ccagcgttgt gccagttcct gaggtgtcag tgagctgatg gtatcagttt
    12841 ccttacctgc atggatccag gaagacatac caccatccag gtatccgatg cagtggtcgt
    12901 atcctacacg ggccatgcgg gtaatggctt ctttttcacg ccccggttca gcaatgagca
    12961 ggatctcttg tttgatatca ggaacaagtg tacctaccca aactgcgaaa ttgccatcga
    13021 ggctgatgtt gatggcattc gggataaatc ctgaggcaaa tgactgctgt tctctggtat
    13081 ccagaatgag ggctcctgtt tcattggcag cagcttcaaa tgcagccggc gagagaggtt
    13141 ggctgcctct gttcatgacg tcatcaaact ggtcataacc tccgatattc atcagtacgt
    13201 tcttggggaa ataaccggga ggaggagtca ggccggtcag cagttcctgc atgaactctt
    13261 cacgggtcat atctgcacgc aaggcatagt tggtcgcttt ctggtggccc agtgtatcac
    13321 tggtttcctt gctcatattc tttccgcagg cagagcctgc tccatgagca ggatagatga
    13381 tcaggtcatc ggtcaaaggc atgatcttat tgcgcagtga atcatagagc atagatgcca
    13441 gcttttcctg ggtcagttct gcgatcactt tctgagccag atccggcctg ccaacatcgc
    13501 caatgaacaa ggtgtcgcct gtaaacagag ctttctctct gccctcttca tcgatcagca
    13561 gaaaacaact gctttccatg gtgtgtcctg gtgtatgaag cagtctgatc ctgatattgc
    13621 ccagttcaaa ttcctggccg tctgtagcca cgagcatatc aaaaccagtc ttcatagtgg
    13681 tggggccata cacgatggtt gctccggttt tttgagccag gtccacatgg cctgaaacaa
    13741 aatctgcatg aaaatgggtt tccagaacgt acttgatcct ggccttgcga tcttcagcca
    13801 tttgaatgta aggggctgtt tcacggagcg gatcaataat agcggcctcg ccgttgcttt
    13861 cgatgtaata tgcaccatgt gccagacatc cggtatagat ctgttctata tacatttttt
    13921 cagatttggt ttactattag agggaagttc atggttgggt atccttactt agatgaagac
    13981 aaaattacct ggtctatccg gtagcttgtg tgatatttgt catgttcacg agatgactta
    14041 aaaagttctg ttttctcctg ttggtttgtt ggcaatgccc taccttgctt attttggcct
    14101 tttacggtct gactcagtga tgtgaacagc ataaaagcag accatagacg ttaaaaagag
    14161 atcagctatg cagaaattca ttcccatttt tccactaggg atggttgcct acccgggcga
    14221 acaattgaac ctgcacattt ttgaagagcg atacaagcaa ctcatcaatg agtgtgtgca
    14281 ggaaaataaa agttttggta ttccaccggt agaaggtaag gaggtgctgc tgtatggcac
    14341 ggagttgttg gttaccgaag tggtaaaaaa gtatgacaat ggtgagctgg atgttcgtgt
    14401 ggaaggaacc ggggttttca ggattctgga ggtgatccat gagattcctg aaaagctata
    14461 tatgggtgct attgtcactg tactggacaa tatcaatgac aggcatatgc ctacccgtga
    14521 acggctgaga gatgctgtaa atgagttgtt cgcattgctg gatattgata aaaggatact
    14581 gccggaagag caggcttact attcctatgc actggcgcat ttggtgggtt ttgatctgct
    14641 gcaggaattt gagttactga agcaccagag cgagagcgtg cgccagcgta ttatactgga
    14701 acatgtgcgc aatctgctgc cgtcggttcg ttctgtgcat gatatcagag agcgggcaag
    14761 gcttaatggt cagttccgga tgataaatcc ccctgacggg atataaaaaa aagccgcctg
    14821 aacaaggcgg ctttttttga aaatctgtaa cagtcttatt tgctggcggt ctggccggga
    14881 taaaccgttc ttgtctgacc attgtagctg gttacttcta cgctgcttac cagatcgctt
    14941 acacgaatga actttgcgct ctgactcagg tagccagatc ctgcctgtag ttcttgcctg
    15001 cgcacttctc cgttcttcag ggttaccatt gcaaatgcat cagattcatt gagcggaatg
    15061 gtcttcacca cagaggtcag gccatagctc agcatattgc catcactgtt gcccagcaga
    15121 tacacaggct ggttattggc accaaccaga atggtggcca tgcctttggc atcaccatca
    15181 ttcctgaatc cggacgaaat gcttctttcc gggctgaact tgcctttgcc attattcagc
    15241 aacaggatac ctgttccggc atcatggcgt gtgatctcga tctctgtatt gtagaagtta
    15301 ccctgtgcca ggatgtccat gtatccgtca ttgttgaagt catcaaccac cataccgaac
    15361 agcacggaga tctgtgcctc attgggcaga taactcattt tgaacttccc atttccttca
    15421 ttcatcagta cagaagaatg gaagatatag gcatccttat gaactgattt ttcaatggct
    15481 tccttaccga gaatttcggt gacggtggct ttaccaaatg catcccatgt ggggaattta
    15541 tctccaatgg acggcatttg ttcaatcagt ctttcccttg tcttaaccgg gtacagctga
    15601 tcatgctgat agtaggccat gatgaagtct tccttaccgt tgctgtcaaa gtcagccagg
    15661 aatgcttcca gcggcagggc acgaccatcg gcttctgaac gtgtattccg gtaacgtctg
    15721 ttggtaccga agttacctac cacgtagtcc atatcaccgt cgttatcaaa gtctgctgct
    15781 gcgatactct gccaccatcc actggtattg accataccgg tctgggctgt tacatcagca
    15841 aatttgcctc ctttgttttg caggaaggtg atgggcatcc aatctccggt gatgatgaga
    15901 tcctgttcat tatcattgtt gaagtctgtc cacaaagcgg aggtcaccat acctatctct
    15961 ttcagggcag gggccagttt gtctgacacg tcttcaaatt tgccgttgtt gttctgcagg
    16021 atatagctgc tggtgctggc cagataacga ccaggcactt gtctgccacc tacaaacaga
    16081 tccatgtcgc cgtctttgtc aaagtcggca gcggtaatga cagaactgct ggaataggta
    16141 gctgggatgg cctctgcatt tctggtgaag ctgcctttac catcattgat ataaagcctg
    16201 tcagcgtacc gtttgtccat atcaggatat tcattaccac ctgttgcgat atagaggtcc
    16261 atatctccat cgctgtctgc atcaaagaag agggctcctg catcttccag ttccaggtca
    16321 tctggttgtt tggtctggaa gtcacttctt tcaaaacttc cgtccgggcg ctgcgtatag
    16381 atcgcacctg actgaccgat ggcaccacct atatagaagt cctgacgacc gtcgccattc
    16441 acatcagcta ccgcgattcc gggagcgagt gagctcattt tatgaggcag caggtattca
    16501 cggaggtaat catcataatc atcttctgtg tgtttgaaat cagtgcccag gtcctgggtc
    16561 atgtttacaa acagttgatc tgttcttccg tgatcatcga atctgatatt cttctgagca
    16621 tctttatgag acagtgtaat ggtctggttt gcagttacat ctcgcagtac ctgtttagca
    16681 ccgtcaagcc attctacttc tacaacgtca accttttcat gagagcccag accgaagtgg
    16741 acatcgttct ccattgtagt gatatagccc ctgacgttgg agtgctgaat gaactgctgc
    16801 tggccatcaa cccagacacg aactttggtt ccgagtccca tggcgttggc gccatagcct
    16861 tcaaactgga agttgatgta attctgctta tcgaatttct ctgagttgtt gcgatagatc
    16921 catggagttt cattcacgtt actgccaatg atatccaggt cgccatctcc gtccagatct
    16981 gcataccctg caccataggt gatggaaggg taatagatac cccactcatc gcgggaatct
    17041 gtaaaggtca ggtcgccatt gttgcggaaa atgaagttcg ggtgactgag cacatagtgt
    17101 ggaagttttt cccttacttc ataatagaca gagctgtctt caattctgac agcacggcgc
    17161 agtttgtggt atgtctctga ttcatctaca tggaaatcac gaagaaaacc gttggcaatg
    17221 aacaggtctt tccatccgtc gttatcaaaa tctgcaaaca gtgttgacca gctccagtca
    17281 gttgtagcaa caccggcggt acggctgatc tcaccaaaag taccatcgcc attgttcagc
    17341 tgaagtgagt tgctcctgtt ctggtatccg tagccggcat tgatcaggat gcgcattact
    17401 tctacctggc tggacagcat gaaggtcttg taggtgtagt tgtcttccat gtccatatct
    17461 gccacgatga tatcaggata accatcgttg ttgtagtcat tcacatcaga acccatggag
    17521 ttgatggaag tctttttaac agcgttgagt gattcatcgc ggaaggtacc atcgccattg
    17581 ttgatgtaga tgaaatcgta catggcatag tcgttagaca cgaatatatc cagatagcca
    17641 tcgctgttca gatcgcctac ggtgccactg agtccatagc catggttgtt gatgcccact
    17701 tccttatgtt tttcagtgta ggtgccatcg ccgttattca cgtagaattt atcgctcagg
    17761 ttaacacctt tttcgatctt ctggaagtat ttcgtcttgt tcttgtcaat gaaatctacc
    17821 gggtgggtaa ccagatacat gtccaggtcg ccatcgttgt cgtaatcaaa gaaatctgca
    17881 tggctggaat agctgtcatc gtcaaggccg tatttggcag cctcttcagt gaaatgcata
    17941 ttgccatcgt ttacccataa ccggttcttt ctttttt
//
LOCUS       DAOMAX010000022        17845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_334_length_17845_cov_4.344969, whole genome shotgun sequence.
ACCESSION   DAOMAX010000022 DAOMAX010000000
VERSION     DAOMAX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17845
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_17845_cov_4.344969"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1650)
                     /locus_tag="PK742_02430"
     CDS             complement(127..1650)
                     /locus_tag="PK742_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQU75475.1"
                     /translation="MKKLASLFLFCTPWLVQAQIPAFLTDSIDQFMNREMARWQIPGA
                     AIAIVKDGQILISKGYGMTDLENGHPVNDETLFMIASNSKAFTGTSLALLQDEGKLHL
                     DDHVTDYLPWLDLYDDCATDMVTIKDMVTHRIGLQTFQGDFLHWGSNLSRRELLEKMD
                     VHQPQYDFRSGYGYCNMGFVAAGEIIGEVSGMSWDDFVSTRIFSPLHMKRSSTTYEAI
                     IADKNACSAYTLNQGNLEKIPYENINNLGPAASINSCVKDLSNWLIMNSQEGSFEGKQ
                     LFPASVIEAATTPYNIVSENRGGLYAAQHFELYGLGWFMADYNGKKMVWHDGGANGFV
                     TTTCFLPELDLGIVVLTNTDENWFYEAMKMQVIEAFLDMPYRNISEMYNTFYVRSRKR
                     QAEETQALLNKAHGNGAVAKHALNEYAGNYISDVYGMASIKLEDGMLHIYFEHHPDLS
                     GTLEPLGGNDFVCTYNQSIYGIKQVSFDEDEAGISAYHMTVADFIDMMEYTFNRVRR"
     gene            complement(1647..2267)
                     /locus_tag="PK742_02435"
     CDS             complement(1647..2267)
                     /locus_tag="PK742_02435"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HQU75476.1"
                     /translation="MRDTNSDNILTDLYYCPPVSWFMLIRQAKALQFDVYRHYQKGGW
                     LNRCYILGPNGMQLLSVPLEKGKNERRAFRDVRISYEHPWQHIHKRTLESCYRRSAYF
                     EYFEDDLQPIFESKPTYLLDWNMRWLEFCLRALKFHPEITYTEKYVPPGTSQITDLRT
                     TRTDDPRHEEIASKFPDYHQVFSDRFPFQRNLSIIDYLFNQGNKPL"
     gene            2327..3790
                     /locus_tag="PK742_02440"
     CDS             2327..3790
                     /locus_tag="PK742_02440"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HQU75477.1"
                     /translation="MKNLIFCVLAGFSIQVSAQSLENMVSIRTAVFDHPESGQYLEVY
                     TTISGNAIHYERNKKYLYQGGVDVQLVLLQGTEVVNFDKFRLNTPQVVDTNSIDFSLI
                     DQQRMVIPNASLSMEVTVTDILDSLNTFTYTEALLPLDTAGLRFSDVQLLDTFYRSAG
                     ESAFLKNGFEMKPYTLNFFPDSRSHLRFYGEVYGTETLLNDPQFLLTFHIESASGNIS
                     SQQFFQHAKVQAGPVVSYLRDIDITDLPSGNYNLVVEVHDRQNEVIGKKKIFFQRAKK
                     GAVSAYDNLELISTDGTFVDDYSSEQLSYFLDVIIPRSTADEAKLIASLSPRVEDEMK
                     KKFLYNFWLDRNPDDPYGEWKRYLQLVKEANDAFSTPSRQGYRTDRGRVYLEYGRPYD
                     RVNSVNEPGAYPYEIWFYTVLPDKQTNIGFAFYEPSMVSNDYILLHSNARGELNDPRW
                     KIRIYENVASPQEIFDFDNTDVQDRTNVYRAIDAYDF"
     gene            3814..4878
                     /locus_tag="PK742_02445"
     CDS             3814..4878
                     /locus_tag="PK742_02445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU75478.1"
                     /translation="MDPADILEHPHVAVVILTWNGREFLKTFLPSVLASTYPSLDIIV
                     ADNSSTDDTITFLRQQFPSVRIIQTGGNLGFAKGYNKALEEVRADYFILLNQDVEVTP
                     GWIEPLVDAMDTDSEWGALMPKIRSYHHRDHFEHAGASGGMIDSLGYPFCRGRIFDKV
                     EADTGQYEEPMEVFWASGAALCIRPALYKRLKGFDEDYFAHMEEIDLCWRLKRAGYKI
                     GVIPSSVVFHVGGGSLKKEDPRKIYLNFRNSLSMLYKNLDFLELVWKLPLRMLVLDVI
                     ALLKSLMEGKWREAWAIIRADWYFFWSWPRQVYKRNTTRKRIQHHAIGFSRVKESGYY
                     KGSIVWQHFIRKKDHYSDLY"
     gene            complement(4870..5295)
                     /locus_tag="PK742_02450"
     CDS             complement(4870..5295)
                     /locus_tag="PK742_02450"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQU75479.1"
                     /translation="MKICIVNGPNLNLLGTREPDVYGPTSFSHYLDDLRERYANQEIS
                     YFQSNHEGELIDHLHEVGFSAHGIILNAGALSHYSYSLADAISAIKSPVIGVHISNIY
                     ARETFRHTDVLSSRCAGVISGLGLQGYEMALQFLLQRSV"
     gene            5399..6319
                     /gene="xerD"
                     /locus_tag="PK742_02455"
     CDS             5399..6319
                     /gene="xerD"
                     /locus_tag="PK742_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQU75480.1"
                     /translation="MNWELAISEYLTWIRLEKGLSANSVEAYRRDVQKLQAFVKRHFP
                     DTTPDTVSSEQLRSFVAELHELGLNDRSQARVVSGVRSFFRFLLTDDQITTDPAELLE
                     LPKLRRKLPDTLSYEEIENIIGALDLSKPEGMRDKAIIDTLYGCGLRVSECIGLQITH
                     IHKELGFIRVIGKGNKERLVPVGDKTLKSIDLYRQFVRNQQSVARGFEDHVFLNRNGK
                     ALSRVYVFMMLRKAAQLAGIRKTISPHTMRHSFATHLLEGGADLRAIQEMLGHESITT
                     TEIYTHLDKTFLRDTLIQFHPGFRKSLSGR"
     assembly_gap    6541..6640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6680..6763)
                     /locus_tag="PK742_02460"
     tRNA            complement(6680..6763)
                     /locus_tag="PK742_02460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6727..6729),aa:Leu,seq:gag)
     gene            complement(7010..7228)
                     /locus_tag="PK742_02465"
     CDS             complement(7010..7228)
                     /locus_tag="PK742_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HQU75481.1"
                     /translation="MRQLKYMVETGVFGVCAYWGDKLGIAASRIRLFFVYATFMTLGS
                     PLIIYMSMAFVLKLRDYMRGFMSPLRDL"
     gene            complement(7235..8500)
                     /locus_tag="PK742_02470"
     CDS             complement(7235..8500)
                     /locus_tag="PK742_02470"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HQU75482.1"
                     /translation="MNEALFQFIWKMRLFDHAALNTTDGEAVDILHPGTQNHHAGPDF
                     SHARIRIGDTLWAGNAELHLRSSQWKEHGHHHDPAYNNVILHVVLEKDSEMPAIPTIE
                     LGKRIKPELLDKYRQWMHSGRWIPCAGSLPVATDTELLSWLGRLAVGRLEDKAMVIAQ
                     RLEMNRGDWDETAWQLIARALGNPVNAEPMEQVARSIPLKLLQKHSHDPLMAEALLLG
                     QAGMLEAGFQELMPLELQSRYRHLRKLHGLESLPPGQWKFGRLRPAHFPGVRLSQLAA
                     IVQETPRLFAAIQKADYKTLQQMFQTEASAYWKEHVHLKHASVRHSARLGKGTIDSIL
                     INAVVPLLLLWGQQQADDPGVERALDLLEQIPAEDNEIIRKWKKHGIQARHAGDSQAL
                     LQLFKTFCNQKRCLSCPIGYRHLQKVTYI"
     gene            complement(8547..9362)
                     /gene="pyrF"
                     /locus_tag="PK742_02475"
     CDS             complement(8547..9362)
                     /gene="pyrF"
                     /locus_tag="PK742_02475"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQU75483.1"
                     /translation="MKRQELADTIKSHRNFLCVGLDTDLQKVPQKFLKEKYPLFAFNK
                     EIIDATRDHCVAYKPNVAFYEAYGSKGWEQLEMTASYLPDTHFRIADAKRGDIGNTSA
                     RYAEAFFKQMPFDAVTVAPYMGEDSVTPFLSFDDKWVIVLGLTSNKGSADFQHMQENG
                     ETLFERVMRRTAAWGDTGNTMFVVGATHPEAFAGIRKIIPDHFLLVPGVGAQGGDLRA
                     VAQSGLNTDGGLLINSTRDIIYASSGDDFQEAAAAKASILAAEMSSLLAEQNL"
     gene            complement(9359..9610)
                     /locus_tag="PK742_02480"
     CDS             complement(9359..9610)
                     /locus_tag="PK742_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQU75484.1"
                     /translation="MSSDTAIYEQVESSLDTIRPYLKADGGDIEIVEITEDLVVRVRL
                     LGACESCPMSFMTMKAGVEESIRSAIPQIKGIETVSAVS"
     gene            complement(9634..10737)
                     /locus_tag="PK742_02485"
     CDS             complement(9634..10737)
                     /locus_tag="PK742_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQU75485.1"
                     /translation="MPVHETDILKALSYVDDPDLHKDIVTLGMVKDLKVSGNTVSFMV
                     ELTTPACPMKDQIEHACRTAIRKLVSEELEVDITMGARVTSTRQQDQDILPGVRNIIA
                     VCSGKGGVGKSTVSVNLALGLAKSGAKVGIMDADIYGPSVPIMLDVRNEKPKVKQEDG
                     KQMMQPVEKHGLKILSIGFLVDDTQAVVWRGPMVSSALRQFVTDTNWGELDYLIIDLP
                     PGTGDIHLTMVQTMPVTGAVIVTTPQEVALADARKAAAMFHMANITVPILGVVENMAW
                     FSPPELPQNKYYLFGKDGGQQLADELKVPVLGRIPIVESIREGGDTGNPVMLDQTNPV
                     QEAWEDLVFNTARYIAIRNEELQPTKKVDIKYT"
     gene            10883..13213
                     /gene="topA"
                     /locus_tag="PK742_02490"
     CDS             10883..13213
                     /gene="topA"
                     /locus_tag="PK742_02490"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQU75486.1"
                     /translation="MTKNLMIVESPAKAKTIEKFLGKDFTVKSCYGHIRDLDKGKDSI
                     DYEKDFKPRYIIPADKKDVVKELKKLAKSAEEIWLATDEDREGEAISWHLCEVLGIDP
                     KTAKRIVFHEITKTAIQHAVDQPRQIDLNLVNAQQARRVLDRIVGFELSPLLWKKVNL
                     SRSLSAGRVQSVAVRILVEREMEINGFEATSSFKVSARLIAKEGKKKHKVKAELPDKL
                     ATEEQAAAFLDSCNGAVYTVDDVSVKPGKRSPSAPFTTSTLQQEASSKLGFSVARTMM
                     IAQKLYEEGHITYMRTDSVNLSEQAISAAADEISKKYGKEYSEPRRYKTKSGNAQEAH
                     EAIRPTYIEIEEAGSSADEKKLYKLIWRRTMASQMAEARLEKTTVNIDISTAKEFLVA
                     KGEVILFDGFLKLYTDSRDEDSEQEDSGILPPLKAGDVLDLEDMQAVQRYTKHPPRYT
                     EASLVKKLEELGIGRPSTYAPTISTIQKRGYVEKRDKEGTPRAYVQLQLTDGKVEREV
                     LSENTGAEKAKLFPTDLGILVNEFLVQYFGEILDYGFTASVEEQFDEISVGHQDWPEM
                     IREFYDPFSKTVKDTEANAQRVKGERILGTDPQSGKQVKVLMGKYGPMAMIGEAEDEE
                     KPRFAGLLKDQDLQSITLEEALKLFDLPRTVGSRDGEDIVAAIGRFGPYLRYKGKFYS
                     IPKTHDPISISLEDSLEVIRAKDEKDANIMIHNWEKEGIQVLNGRYGPYIKVGKLNYK
                     IPKGETAEHLSLDRVKEIIADSEPTGKGRRFSRKGS"
     gene            13213..14097
                     /locus_tag="PK742_02495"
     CDS             13213..14097
                     /locus_tag="PK742_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQU75487.1"
                     /translation="MAEASEREIKALIALLDDTDREVFEHVSDKLFSMGPGVIGVLED
                     AYTSIADQLTQQRIENLIHSIQFSLVKKELTEWMHSEPDDLLKGLLIMNRYQYPLADE
                     DALMQRWQQFRKDIWLGLNMYLSPLEQIDVVNQVLFGQGGLTGTDEEKDAARFSFIGH
                     VLESGRGNHFSIGLIYLALCQQLGLPVYGVLLRSHFILVRCREEILQIRDLNTLIDEV
                     LFYINPYNRGLTFSEREIRNYIEKAGLEPVKHYFLPAGNLEVMAEYLRFLQDQFTESG
                     AQWKCDDLEQLIHLLDKA"
     gene            complement(14098..15187)
                     /gene="prfB"
                     /locus_tag="PK742_02500"
     CDS             complement(join(14098..15126,15128..15187))
                     /gene="prfB"
                     /locus_tag="PK742_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131330388.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQU75488.1"
                     /translation="MTSEHYRDLQDKLLVLRRYLNYDDRIHQAQEDEQITQQPGFWDD
                     PKNAEQVLKRIKINNLFINQYKSAQTAVDDLGVLQEFVQASEATEEELETAYKEAVEL
                     VEELEFKSTLDEAEDQMSAILTINSGAGGTESCDWAGMLYRMYNMWAQKSGFKVTELD
                     YQDGDVAGIKSASLEIEGEFAYGLLKSENGVHRLVRISPFDSNARRHTSFASVFVYPM
                     IDDTIEINIHPNDLEWDTFRSGGAGGQNVNKVETAVRVTHLPTGTVVACQVARTQGQN
                     RDMAMQMLRSKLYEMELQRRQAEKDKVEGTKKKIEWGSQIRNYVLHPYKLVKDARTGE
                     ETGNTQAVLDGEIDNFIKAYLLWDGKEK"
     gene            15266..16891
                     /locus_tag="PK742_02505"
     CDS             15266..16891
                     /locus_tag="PK742_02505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HQU75489.1"
                     /translation="MSANSREQSIEKLESEVFDLVVIGGGITGAGIALDAASRGLSVA
                     LLEKYDFAGGTSSRSTKLIHGGLRYLKQLELGLVRKVGRERAVIHRLAPHLVRPVPML
                     LPVYRNGSLGRWSTTLALSVYDRLAGVKKQERFKWYGPKEVANMEPGLLTDELQGGAV
                     YTEYRTDDARLTMSVLKSASGAGAVCLNYMLVKDFVRENNRLSAVLADDLIGGKMITV
                     RGKLVVSAAGPWVDEIRRIENKSSDKKLQLTKGVHLVLKAERFQINYPVYADVGDGRM
                     FFIIPRGNRVYIGTTDTLYAGPTENPQVTKDDADYLLAAANRLFPKQHLTVKDIISSW
                     AGVRPLLFEEGKSPSELSRRDEIWETSSGLISIAGGKLTGYRTMAKEVVDKVMFKLTG
                     SKSVCKTESLLLDGYVSEQELEALITRLKGEASQVGFSHEEIRRLVFTYGSETELIIE
                     QAYTLHQEVKDQEKRILQAELMYCVQFEWVRNISDFFIRRTGMLYFDRQRVLKQYLPA
                     AEIIAELLDQQDGQTREDIAFLEAALSQAVTFN"
     gene            16923..17606
                     /locus_tag="PK742_02510"
     CDS             16923..17606
                     /locus_tag="PK742_02510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU75490.1"
                     /translation="MHTYLVVGGSSGIGYETCRQLLSAGHQVVVIGRNKPDLPVTFFQ
                     SDVVANDTLPVWDEPLNGLVYCPGSIRLKPFKGLTASDFEEDLRINYLGLVKVLHQYH
                     PALKRSGQASVVVFSTVAVKTGMPFHASIAGAKGAVEGLARSLAAEWAPSIRVNVVAP
                     SLTDTPLAERLLDSDSKREASAARHPLKQIGDAKNTASLVSWLLSDDSVFMSGQVLRP
                     DGGISSLKV"
BASE COUNT         4623 a         4341 c         4330 g         4451 t
ORIGIN      
        1 atgctgccca tccccaatca ttatttctgg ccccgcaggt gaatcccagc acttccgtgt
       61 agaaccggat gctctcatcc agatcgcggg tatacagaat aggcctgagt cgggtcagta
      121 ccatatctat cttctgacgc gattaaaggt atattccatc atgtcaatga aatctgccac
      181 ggtcatatgg taggctgaaa tgcctgcttc atcttcatca aaactgacct gcttgatacc
      241 atatatgctt tgattgtagg tacatacaaa atcatttcct cctaagggtt caagcgtacc
      301 cgacagatcc ggatggtgtt cgaagtagat atgcagcata ccgtcctcca gtttgatact
      361 ggccatgcca tacacgtcac tgatgtagtt acctgcatat tcgttcagtg catgtttggc
      421 tacagctccg ttgccatgtg ctttattcag caaggcctgg gtctcttctg cctgtctttt
      481 tctgctcctg acataaaagg tattatacat ctcgctgata ttgcgataag gcatatccag
      541 aaaggcttct attacctgca tcttcatggc ttcatagaac cagttctcat ccgtattggt
      601 cagtaccaca ataccaagat ccagttcagg caggaaacag gtggtggtaa caaaaccgtt
      661 tgctccgcca tcatgccata ccattttctt gccattatag tcggccatga accagcccag
      721 tccatacagt tcaaaatgct gggcagcata aagaccaccg cggttctcgc ttacaatatt
      781 atagggcgtg gtggcggcct ctatgacaga agccggaaac agctgcttac cctcaaagga
      841 accttcctgg ctgttcatga taagccagtt ggacaagtct ttcacacagc tgttgatgct
      901 ggctgcaggg cccaggttgt tgatgttttc ataaggtatc ttttccaggt ttccctggtt
      961 cagggtatag gctgaacagg cattcttgtc tgcaatgatc gcctcatagg tagtgctgct
     1021 gcgcttcata tgcaagggac tgaagatgcg ggtgctgaca aaatcatccc agctcatacc
     1081 gcttacttcg ccaatgatct ctccggctgc aacaaatccc atattacaat agccataacc
     1141 acttctgaaa tcatattgcg gttggtgtac atccattttt tccagcaact cacgcctgct
     1201 gaggttgctt ccccaatgga gaaaatcacc ctgaaaggtt tgcagtccaa ttcggtgggt
     1261 taccatgtct ttgatggtta ccatatctgt cgcacaatcg tcatacagat caagccaggg
     1321 caggtagtct gtcacatgat cgtctaaatg gagtttgcct tcatcctgca acagagccag
     1381 tgaggttcct gtgaaagctt tggaattgct ggctatcatg aaaagtgttt catcattgac
     1441 gggatgacca ttttccagat cggtcatacc atatcctttg cttatcagta tctgcccgtc
     1501 tttgacaatg gcaatggctg ctcccggtat ctgccagcgg gccatttccc ggttcatgaa
     1561 ctgatcgatg ctatcagtca ggaaagcagg aatctgtgcc tgtaccagcc agggagtgca
     1621 aaaaagaaaa agtgaggcta attttttcat agtggtttgt tgccttgatt gaagaggtaa
     1681 tcaatgatac tcaaattgcg ctggaatggg aaacgatcac tgaacacctg gtgataatcc
     1741 ggaaactttg aggcgatctc ctcatgtctt gggtcatcag ttcttgtagt tctcagatct
     1801 gtgatctggc tggttccggg tggtacatac ttctcagtat aggtgatctc cggatgaaac
     1861 ttcaaagccc tcaggcagaa ttccagccat cgcatattcc aatccagcag ataggtcggt
     1921 ttgctttcaa atattggttg aaggtcgtct tcgaagtatt caaaatacgc tgaccggcga
     1981 taacatgatt ccagtgttcg tttgtgaatg tgctgccagg ggtgttcgta cgaaatgcgc
     2041 acatcacgaa aggctcttcg ttcgttcttg cccttttcca ggggtactga cagcaactgc
     2101 attccattcg gtccgagtat atagcaccgg ttcagccaac cgcccttctg gtagtgtctg
     2161 tacacatcga actgcagggc ttttgcctgc ctgatcagca tgaaccagga taccggaggg
     2221 caataataca ggtcggtcag tatgttgtca gaatttgtat ccctcatgcc tgtgaacgta
     2281 actgcttcac aacgaaattc gtaattttag acagaaacat catcagatga aaaacttgat
     2341 cttctgtgtg ttggcaggat tttccatcca ggtttctgcc cagtcacttg aaaatatggt
     2401 cagcatccgg accgccgttt ttgatcaccc cgaatccgga caatatctgg aggtctatac
     2461 aaccatttcc gggaacgcca ttcactacga acgcaacaag aaatatctgt accagggtgg
     2521 tgtggatgta cagctggttc tgctgcaagg taccgaagtg gtgaactttg acaagttcag
     2581 actcaataca ccccaggtgg ttgataccaa cagcatagat ttctctctga tagatcagca
     2641 gcggatggtc attccaaatg catctctgag tatggaggtg acagtaactg atattctgga
     2701 cagcctgaac acctttacct atacagaagc tttacttccg cttgatacag cgggtctgag
     2761 attttcagat gtacagttgc tggatacttt ttacaggagt gcaggcgaaa gcgctttttt
     2821 gaagaacggt tttgaaatga aaccatacac tttgaatttt tttcctgaca gccgcagcca
     2881 cctgaggttt tacggagaag tgtacggtac agaaacactg ttgaatgacc ctcagttcct
     2941 gctaacattt cacatagaat cagcaagtgg taatattagt tctcagcagt tctttcagca
     3001 tgccaaagtg caggcaggtc ctgttgtttc ttacttacga gacattgata tcacagatct
     3061 gccttccgga aactacaacc tggtagtaga agtacatgac cggcagaacg aagtgatagg
     3121 caagaagaag atctttttcc aacgggccaa gaaaggagcg gtttctgcct atgacaatct
     3181 ggaactgatc agtacggatg gcactttcgt ggacgattac agcagtgaac agctcagcta
     3241 tttcctggat gtcatcatac ccagaagtac agcagatgaa gccaaactga tcgcttctct
     3301 cagccccagg gtggaagatg aaatgaaaaa gaaatttctg tacaacttct ggctggatcg
     3361 taatccggac gacccatacg gcgaatggaa gagatatctc cagctggtca aagaagcgaa
     3421 tgatgcattc tctactccat ccagacaagg ctatcgcact gacaggggaa gggtgtacct
     3481 ggagtatgga aggccatatg acagggtcaa ttcagtcaat gaaccaggtg cctatcctta
     3541 tgagatctgg ttttatactg tattacctga taaacagacc aacattggtt ttgctttcta
     3601 cgagcccagc atggtctcca atgattatat cctgttgcat tccaatgcaa gaggtgaact
     3661 caatgaccca aggtggaaga tcaggatata tgagaacgta gcttctccgc aagaaatttt
     3721 tgactttgat aatacggatg tgcaggatcg taccaatgtt tacagggcca ttgatgccta
     3781 cgatttctga gtacacagta tcttcgtttg gtgatggatc cggccgatat tcttgaacac
     3841 ccgcatgtgg cggttgtgat cctgacatgg aatggcaggg aatttctcaa gaccttcctg
     3901 ccttctgtgc ttgcttctac ttacccttca ctggatatca ttgtagctga caacagcagc
     3961 acagatgata ccatcacttt tctccgtcaa caatttccgt ctgtcaggat cattcaaacc
     4021 ggtggtaacc tgggctttgc caaaggatat aacaaagcac tggaagaggt cagagcagat
     4081 tatttcattt tgttgaatca ggatgtggaa gtaactcctg gttggataga accactggta
     4141 gatgccatgg ataccgattc tgagtggggg gctttgatgc cgaagatcag atcatatcac
     4201 cacagagatc actttgaaca cgcaggtgct tccggtggaa tgatagattc gcttggatat
     4261 cctttttgcc ggggacggat tttcgataag gtggaagctg ataccgggca atacgaagag
     4321 cctatggaag tattctgggc aagtggtgcc gcactttgta tcaggccggc tctgtacaag
     4381 agactcaaag gctttgacga agactacttc gcacatatgg aagagatcga tctctgctgg
     4441 cgtttgaaac gggccggcta caagatcggt gtgataccgt catcagtagt gtttcacgta
     4501 ggaggcggaa gtcttaaaaa agaggatcca cgaaagatct atctcaactt ccgcaattca
     4561 ctcagcatgc tctacaagaa ccttgatttt ctggagctgg tgtggaagtt gccgctgcgt
     4621 atgttggtgc tggatgtgat cgccctactc aaatcactga tggaaggcaa atggagagaa
     4681 gcatgggcca tcattcgtgc cgattggtat ttcttctgga gctggccaag gcaggtgtac
     4741 aaaagaaaca cgaccaggaa acgtatccag caccatgcaa ttgggttctc cagggtgaaa
     4801 gaatccggct attacaaggg aagcatagtc tggcagcatt tcatcagaaa aaaggaccac
     4861 tactcagacc tatactgatc gttgcagcaa aaattgcaga gccatctcat acccttgtaa
     4921 acccagacca ctgatcacgc ctgcacatct ggatgacagc acatctgtat gcctgaaagt
     4981 ttcacgtgca tatatgttgc tgatgtgtac acctatcaca gggcttttga tggcacttat
     5041 ggcatctgca agcgaatagg aatagtgaga aagggcacct gcattcagta tgattccatg
     5101 cgctgaaaaa cccacctcat gcagatggtc tatcaattct ccttcgtgat tggactgaaa
     5161 atagctgatc tcctggttgg catatcgctc ccgcaggtca tccagatagt gactgaaaga
     5221 ggtcggtccg tacacatccg gctcgcgggt acccagtaaa ttgaggttcg gtccgttcac
     5281 gatgcagatc ttcataaatc aaaggtacac cttgccaggt aaacaataag ttggtcaact
     5341 gccgttttcc tgttgacatc ccggggtatt gaccctttgg tgtcaggtaa attgcagcgt
     5401 gaactgggaa ctcgctatat cagagtattt gacctggatc cgtctggaaa aagggctttc
     5461 tgccaatagc gtagaagctt acaggcgtga tgtgcagaaa ctgcaggcat ttgtgaagag
     5521 gcattttcct gacacaacac ctgataccgt cagctctgaa caactccggt cgtttgtagc
     5581 tgaactgcac gaacttggcc tgaatgacag aagtcaggcc agggtggtga gcggcgtcag
     5641 gtctttcttc cgatttctgc tcactgatga tcagatcacg acagaccctg ctgaattgct
     5701 ggagctgcca aaactccggc gtaaactgcc ggacaccctg agctacgaag agatcgaaaa
     5761 tattatagga gcactggact tgagtaagcc ggaagggatg cgggataaag ccatcattga
     5821 tacattatac ggatgcggat tacgggtaag cgaatgcatt ggtttgcaga taacccatat
     5881 tcacaaagaa ctcgggttta tccgggtaat cggaaagggt aacaaagaaa gactggttcc
     5941 ggttggagat aaaaccctta aaagtattga cctgtacagg caattcgtaa ggaaccaaca
     6001 atctgttgcc agggggtttg aagaccatgt cttcctcaac agaaacggga aagccctgag
     6061 tcgtgtctat gtattcatga tgcttagaaa agcagcgcaa ctggcaggta tccgtaaaac
     6121 catctcaccc cataccatgc gccacagttt tgccacacat ttgctggaag ggggagcaga
     6181 cctgcgggct atccaggaaa tgctggggca tgaaagtatt accacaacag agatctatac
     6241 ccacctcgac aagacattcc tccgcgatac cctgatccag tttcatccgg gatttcgcaa
     6301 gagtttatcc ggccggtaat ggccctctta agaaataaaa aatgcggact acaggcactc
     6361 tgtactccgc actctaacca ttttgtgccc aggactggac ttcccgcctt ccgtgttttg
     6421 aaagatagga gtggcgggat aaacttctcg tccagtcccg tttagccaaa caaaaaatgc
     6481 ggaccacagg cactctgtac tccgcactct aaccattttg tgcccaggac tggacttccc
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaccacaggc actctgtact
     6661 ccgcactcta accattttgt gcccaggact ggactcgaac cagcacagcc ttgcggccac
     6721 taccccctca aagtagcgtg tctaccaatt tcaccacctg ggcaggggac tgcaaaggta
     6781 tgcgaaaaaa gagattgcag aaaatacctg ataaaaaaga cggatgatca tgcctgaaca
     6841 catgatgtca tgcaaccaat aggagtaatc aggcctcttg gtggtaagtg tttggtgacg
     6901 gtttgttcat gtatgcttcc cccgccacgg taacgcatat catttatcag atctaaagag
     6961 caactgtcaa cccaccctga tcgcaggccg aaaggcctgc ttttttttgt cagagatcac
     7021 gtaacgggct catgaaccca cgcatataat cacgaagctt cagcacaaat gccatactca
     7081 tataaatgat cagcggagag ccgagcgtca tgaatgtggc atagacaaag aacagcctga
     7141 tacgtgacgc agcaattccg agtttgtcac cccaataggc gcaaacaccg aacacaccgg
     7201 tctctaccat atatttcagc tgcctcatct cttatcaaat ataagtcacc ttttgcagat
     7261 gtctgtaacc aataggacag gataggcaac gcttttggtt gcaaaatgtt ttaaacaatt
     7321 gcagaagagc ctgtgaatcg cctgcatgcc tggcttgtat gccatgtttt ttccactttc
     7381 ggatgatttc attgtcttcg gccggaatct gttccagcag atccagcgcc cgctctacac
     7441 cgggatcatc tgcctgctgc tgcccccaaa gcaggagcag cggcactacg gcattgataa
     7501 gaatgctgtc aatggtcccc tttcccaacc tggcagaatg tcgaaccgat gcatgtttca
     7561 ggtggacatg ttctttccag taggcagaag cttctgtctg gaacatctgc tgcagggtct
     7621 tgtaatctgc tttctggatg gcagcgaaca aacgcggtgt ttcctgcaca atggccgcca
     7681 gctgacttaa tctgacccct gggaaatgtg ccggcctgag cctgccaaac ttccattggc
     7741 ccggtggcaa agattccaga ccatgcagct ttctcagatg acggtaccgg ctctgcagct
     7801 ccagcggcat cagctcctga aaacccgcct ccagcatgcc cgcctgaccc agcaataacg
     7861 cctccgccat caatggatca tggctgtgtt tctgaagcaa ttttaaagga atactcctgg
     7921 ctacctgctc catgggctca gcattgacag gatttcccaa tgcacgggca ataagctgcc
     7981 aggccgtttc atcccagtcg ccgcgattca tttccagcct ctgtgctatc accatagcct
     8041 tgtcttcaag acggcccact gccaatcttc caagccagga cagtaattct gtatctgttg
     8101 ccaccggaag actgcccgca cagggtatcc accggccgga gtgcatccat tgtctgtact
     8161 tgtccagtaa ttcgggtttg atcctcttgc ccagctcaat ggttggtatc gccggcattt
     8221 cactgtcttt ctccagtacc acatgcagga taacattgtt gtaggcagga tcgtggtggt
     8281 gaccatgctc cttccattgc gagctgcgca gatgtaactc agcgtttccc gcccaaagtg
     8341 tatcgcctat gcgaatacgt gcatggctga aatcagggcc tgcatgatgg ttctgggtgc
     8401 cggggtgcag tatatctacc gcctcaccgt ctgtggtgtt taaagcagca tgatcaaaca
     8461 gccgcatttt ccagatgaat tggaataaag cttcgttcat gagggtaagc atgagcgatg
     8521 gaaagatatt aaaaaaacgg aatagatcaa agattctgct cggcaagcag gctgctcatc
     8581 tcagcagcca ggatcgaagc ttttgctgca gcagcttcct ggaagtcatc gccggaagat
     8641 gcgtaaatga tatcacgtgt tgaattgatc agtaaaccac catcggtatt caaacctgac
     8701 tgagccactg cacgaagatc tccaccttgt gcaccaactc cgggtaccag caggaaatga
     8761 tcaggtatga tcttcctgat tccggcaaat gcctccggat gggtggcgcc cactacgaac
     8821 atggtgttgc cggtatctcc ccatgctgca gtgcgtcgca tcacccgctc gaagagggtt
     8881 tctccgttct cctgcatgtg ctgaaaatct gcagaaccct tattggaggt cagccctagt
     8941 actatgaccc atttgtcatc gaatgaaaga aatggggtca cactgtcttc gcccatatag
     9001 ggagcaacgg ttaccgcatc gaaaggcatt tgcttgaaaa atgcttccgc atatcgggca
     9061 gatgtgttac cgatatctcc ccgcttggca tctgctatcc ggaaatgggt gtcgggcaga
     9121 tatgatgcgg tcatttccag ctgttcccaa cccttacttc cgtatgcttc atagaatgcc
     9181 acattcggtt tataagccac acaatggtca cgcgtggcat caatgatctc cttgttgaat
     9241 gcaaacaggg ggtacttctc tttcagaaac ttttgaggga ccttctgcag gtcggtgtcc
     9301 agtcctacgc agagaaagtt gcgatggctt tttatagtat ctgccagctc ctgtctcttc
     9361 atgaaacagc agaaacggtt tctataccct tgatctgtgg aatggcactg cggatggatt
     9421 cttccacacc cgccttcatg gtcatgaaac tcatggggca gctttcgcag gcacccagca
     9481 accgcaccct cactacgagg tcttctgtga tctccacgat ctcgatgtca ccaccatcgg
     9541 ccttcagata gggacgaata gtatccagtg atgattcaac ctgctcgtat atggcggtgt
     9601 ctgaactcat tgtcatacaa aggtacaacg caatcaggtg tatttgatat ccactttttt
     9661 agtgggttgc agttcttcat ttctgatggc gatatacctg gctgtattga aaaccagatc
     9721 ttcccaggcc tcctgaactg gatttgtctg atctaacatg accggattac ccgtatcgcc
     9781 accttcacga atggattcca ctatcggaat tcgacccagc accggcactt tcagttcatc
     9841 tgccagctgc tgcccgccgt cttttccaaa caggtaatat ttgttttgag gtaactccgg
     9901 cggactaaac catgccatgt tctccacaac acccagaata ggtacagtaa tatttgccat
     9961 atggaacatg gccgctgcct ttcttgcatc tgccagtgca acctcctgtg gagtggtcac
    10021 gataacggca ccggtaacag gcatggtctg caccatggta agatgaatat cgcccgttcc
    10081 gggaggcagg tcgatgatca gatagtccag ctctccccag ttggtatctg ttacaaattg
    10141 tctcagtgct gaactcacca tcggcccacg ccagaccacc gcctgggtat catccaccag
    10201 aaacccgatg gacaggatct tcagaccatg cttttctacc ggctgcatca tctgctttcc
    10261 gtcttcctgc ttcaccttcg gtttttcgtt cctgacatcc agcatgatgg gaacagaagg
    10321 accgtatatg tcagcatcca tgatgccaac cttggcacct gattttgcca gacctaaagc
    10381 aagattgaca gatactgtgc tttttcccac tcctcctttg ccggagcata cagcaatgat
    10441 gttcctcact ccaggaagga tgtcctgatc ctgctgacgt gtggaggtca ctctggcccc
    10501 catggtgata tccacttcta attcttcact taccagtttg cggatggctg tgcggcaggc
    10561 atgctctatc tggtccttca tcgggcaggc aggagtggtc agctctacca taaaagaaac
    10621 agtattgcca ctgaccttca ggtctttgac catacccagg gtaacaatgt ctttgtgcag
    10681 atcaggatca tccacatagc tcagggcttt caatatatcg gtctcatgta caggcatgaa
    10741 caaggcttgt tttgcacaaa actataactg aatgttgaat gaattgttta acctttgcgg
    10801 gagatgccgc tcagaagcta catttggtaa tttctgcaca cggttatatt tgcatcatat
    10861 aaaaggaaga ggagaccggt acatgacgaa gaatctgatg atcgttgagt cgcccgccaa
    10921 ggcgaagacc attgagaaat ttttaggcaa agactttacc gtaaagtctt gctacggcca
    10981 catccgcgac ctggacaagg gcaaggattc cattgactac gaaaaggatt tcaagcccag
    11041 atacatcatt cctgccgata aaaaagacgt ggtcaaggaa ctgaaaaaac tggccaaatc
    11101 agccgaagag atctggctcg caacggatga agaccgcgaa ggcgaggcta tttcctggca
    11161 tctctgcgaa gtcctgggca tagacccgaa gaccgccaaa cgcattgtat ttcatgagat
    11221 caccaagact gccatacagc atgcggtaga tcaacccaga caaatagacc tgaacctggt
    11281 caatgcacag caggccagaa gggtgttgga tcgcattgtg ggttttgaac tctctccatt
    11341 attatggaag aaggtgaatc tgtcaagatc actcagcgcc ggccgggtgc agtctgtggc
    11401 tgtgcgcatc ctggtggaaa gagaaatgga gatcaacggc tttgaggcta cctcttcctt
    11461 taaagtaagt gcaaggctga tagccaaaga agggaaaaag aagcataagg taaaggctga
    11521 actccccgat aaactggcta ccgaagaaca ggctgccgct ttcctggatt cctgtaatgg
    11581 agcagtgtac acggttgatg atgtatcggt caaacccggc aaacgcagtc cttcagctcc
    11641 tttcactacc tccactctgc aacaggaagc atcctccaaa ctgggctttt ctgtggcccg
    11701 taccatgatg atcgcacaga agctctatga ggaggggcat atcacctaca tgcgaactga
    11761 cagtgtcaac ctgtctgaac aggccatctc tgccgcagct gatgagatca gcaaaaagta
    11821 tggtaaagag tattctgagc caagacggta caagacgaaa tcaggcaatg cccaggaggc
    11881 acacgaagcc atccgcccta cttatataga aatagaggaa gcaggctcct ctgctgatga
    11941 aaagaaactc tataagttga tctggcgcag aaccatggct tcgcaaatgg ccgaagcaag
    12001 gctggagaaa accacagtga atattgacat cagtactgcc aaagaatttc tggtggcaaa
    12061 aggtgaggtc attttgtttg acggtttcct gaaactctat accgattcac gcgacgaaga
    12121 cagtgaacag gaagactccg gcatcctgcc tccgctcaaa gccggcgatg tactggacct
    12181 ggaagatatg caggctgtgc agcgatatac caaacatccg cccagatata ctgaagcatc
    12241 acttgtcaaa aagttggaag aactgggcat tggcagacct tccacctatg cacctaccat
    12301 ctctaccatc cagaaaagag gctatgtaga gaagcgtgac aaggaaggta caccacgtgc
    12361 ctatgtgcag ttacagctta cagacgggaa agtggaacgc gaagtgctga gtgaaaatac
    12421 aggggctgaa aaagctaaac tttttccaac agacctgggt attctggtca acgaatttct
    12481 ggtgcagtat tttggcgaga tcctggatta cggatttacc gcttctgtgg aagaacaatt
    12541 cgatgagatc tctgtgggtc accaggactg gccggaaatg atccgtgaat tctatgatcc
    12601 tttcagcaag accgtcaagg atacagaggc caatgcacaa agggtcaagg gcgaacgtat
    12661 cctcggaacc gatcctcaat ccggcaagca ggtcaaagtg ctgatgggca aatatggtcc
    12721 catggccatg atcggtgaag cggaggatga agagaaacca cgctttgcag gattgctgaa
    12781 agaccaggac ctgcagtcta tcaccctgga agaagcactc aaattatttg accttccgcg
    12841 gacggtcggc tccagagacg gagaagacat cgtggccgct atcgggcgct tcgggcccta
    12901 cctgcgctac aagggcaaat tttacagcat tcctaaaaca catgatccta tcagcatcag
    12961 tttggaagac tctctggagg tgatcagggc aaaggatgag aaagatgcga atatcatgat
    13021 ccacaactgg gagaaagaag gcatccaggt actgaacggt cgctatggcc cttatataaa
    13081 ggtgggtaaa ctgaactaca agatccccaa aggagaaacg gcagaacacc tgtcgctgga
    13141 ccgggtcaaa gagatcatcg ccgattcaga accaaccgga aaaggccggc gtttttccag
    13201 aaaaggcagt tgatggcaga agcgtctgaa cgggagatca aagcactcat cgccctgctg
    13261 gatgatacag acagggaggt cttcgaacat gtttcagaca aactgttctc tatgggtccg
    13321 ggtgttatcg gggtactcga agatgcctat acttccatcg cagaccagct gacccagcaa
    13381 cgcattgaga atctcatcca cagcatccag ttttctctcg ttaagaagga actgacagaa
    13441 tggatgcaca gtgaacccga tgatctgctc aaaggcctgc tgatcatgaa ccgataccag
    13501 tatccgcttg ccgatgaaga tgcacttatg cagcgatggc agcaattcag aaaagatatc
    13561 tggctgggat tgaatatgta tctttcgccg cttgaacaga tcgatgtggt caaccaggta
    13621 ctgttcggtc agggcggact gacaggaacc gatgaagaaa aagatgcagc ccgattcagt
    13681 ttcatcggcc acgtgctgga atcaggaaga ggtaatcact tttccatcgg gctgatctac
    13741 ctggcactgt gccagcagct tgggcttccg gtatatggtg tgctgctgcg gtctcatttt
    13801 attctggtca gatgtcgcga agagatactt cagatcagag acctgaatac attgatagat
    13861 gaagtgctgt tttatataaa tccgtacaac cgcgggctca ccttcagcga aagagagatc
    13921 cggaattata ttgaaaaggc cggactggaa cctgtgaaac attatttcct gccggccggt
    13981 aacctggagg tcatggctga atatctgcgt ttcctgcagg atcagttcac ggaatcagga
    14041 gcacagtgga aatgtgatga cctggaacaa ctcatccatt tgctggacaa ggcctgatca
    14101 tttttcctta ccgtcccaaa gcagatacgc cttgatgaag ttgtctatct ctccatccag
    14161 tacggcctgc gtattgcccg tttcttctcc tgttcgggca tcttttacca gcttgtacgg
    14221 atgaagcaca tagttcctga tctgagaacc ccactcgatc tttttcttgg tgccttccac
    14281 cttgtctttc tctgcctgac gcctctgcaa ctccatctca tacaatttgg agcgcagcat
    14341 ctgcatggcc atatcacggt tctgtccctg cgtccgggca acctgacagg ctaccaccgt
    14401 accggtagga aggtgggtca cccgcactgc ggtttccacc ttgttcacgt tctgtccgcc
    14461 cgcaccacct gagcggaaag tatcccactc caggtcattc gggtgaatgt tgatctcgat
    14521 ggtatcatcg atcattgggt aaacaaagac ggatgcaaaa gaagtatgtc ttctggcgtt
    14581 gctgtcgaat ggtgagatgc gcaccaatct gtgcacgccg ttctcgctct tgagcaatcc
    14641 ataggcaaat tcaccttcta tctccagtga agcacttttg ataccggcca catcgccgtc
    14701 ctggtaatcc agctcggtca ccttgaaacc gctcttctgc gcccacatat tatacatgcg
    14761 gtaaagcatg ccggcccagt cgcagctttc tgttccaccg gcaccggagt tgatggtcag
    14821 gatggccgac atctggtctt cggcctcatc taaggtggac ttgaattcca gttcttctac
    14881 cagttcaaca gcttccttat aggctgtttc cagttcctct tcggtggctt cgctggcttg
    14941 cacgaattcc tgcagcacgc cgaggtcatc gacagctgtc tgtgcgctct tgtattgatt
    15001 gatgaacagg ttgttgatct tgatccgctt cagcacctgc tctgcattct tggggtcatc
    15061 ccagaatccg ggttgctgtg tgatctgttc gtcttcctgt gcctggtgta ttctgtcgtc
    15121 gtagttaaag atacctcctt aacaccaaca gcttgtcttg caggtctctg tagtgttcac
    15181 tcgtcataat ggcggcaaag atagcatatt gtaccatgcg tacaactact tcgtttgtgt
    15241 agtgaccact aggtagattt gtaccatgtc agccaacagc cgtgaacaaa gtatagaaaa
    15301 actggaatca gaggtctttg atctggtggt catcggcgga ggcattaccg gggctggcat
    15361 agcactcgac gccgcatcac gggggctaag cgtggcactg cttgagaaat atgattttgc
    15421 cggcggcaca tcaagccgct ccaccaagct gatacatggc gggctgcgat atttgaaaca
    15481 actggagctc ggactggtgc ggaaggtagg aagagagcgt gctgtcatcc accggctggc
    15541 accacatctg gttcgtccgg tccccatgct gctgccggtt tatcgcaatg gcagcctcgg
    15601 ccgatggtcc accacactgg cgctctctgt gtatgaccgg ctggccggag tgaaaaaaca
    15661 agaacgtttc aaatggtatg gcccgaaaga agtggccaac atggaacctg gattgctgac
    15721 agatgaactg cagggtggtg cggtctatac tgaatatcga accgatgatg ccagactgac
    15781 catgtccgtt ttgaaatctg cttccggtgc aggagcggta tgtcttaatt atatgctggt
    15841 gaaggatttt gtgcgggaaa acaaccggct cagcgcagtg ttggccgatg atctcatagg
    15901 aggaaagatg ataacggttc ggggaaaact agtcgtatca gccgccggac catgggtgga
    15961 tgagatccgt cggatagaaa acaagagcag cgacaaaaaa ctacagctta ccaagggtgt
    16021 ccatctggtg ctgaaagcag aacgtttcca gataaactat cctgtgtatg ctgatgtggg
    16081 agatggtaga atgttcttca tcattcctcg tggtaatagg gtatatatcg gtacaaccga
    16141 tacgttgtac gccggcccga cagaaaaccc acaggtcaca aaagatgatg cagactattt
    16201 gctggctgca gcaaaccgcc tgttcccaaa acagcacctg acagtgaaag acatcatttc
    16261 ctcctgggca ggtgttcgac ctttgttgtt tgaagaaggg aagtcaccat cagaattatc
    16321 ccgcagagat gagatctggg aaacttcgtc cgggctgatc agcatagccg gaggtaagct
    16381 gaccggctat cgcaccatgg caaaagaagt ggttgacaag gtcatgttta aactgaccgg
    16441 aagtaaaagt gtctgcaaga ccgaatcttt gctactggat ggttatgtat ctgaacagga
    16501 gctggaggca ctgatcacca gactgaaagg agaggcatca caggttggat tttctcatga
    16561 agagatccgg cgactggtct tcacttatgg ctccgaaaca gaacttatta tcgagcaggc
    16621 ctatacgtta caccaggaag taaaagatca ggaaaaaagg atattgcagg cagaactgat
    16681 gtactgtgtg cagtttgaat gggtgcgcaa catcagcgac ttttttattc gccgcaccgg
    16741 catgctgtac tttgacaggc aaagggtatt gaaacaatat cttccggcag ctgagatcat
    16801 tgccgaactg ctggatcagc aagatgggca aacccgcgag gatatcgcat ttctggaagc
    16861 agcactcagc caggcagtga cctttaacta aaaccttttc tgttcttcga tgttcttctg
    16921 ccatgcacac atatttggta gtgggaggca gcagcgggat aggatatgaa acctgccgtc
    16981 agttgctgag cgccggacat caggtggtgg tcattggacg aaacaaacct gaccttcctg
    17041 ttactttttt tcaaagtgat gtggtagcca atgataccct gcccgtctgg gatgagccac
    17101 tgaacggttt ggtatattgt ccgggttcca tcaggctgaa accattcaaa gggttgacag
    17161 cgtcagactt tgaagaagac ctccgcatca actatctcgg gttggtaaaa gtgctgcacc
    17221 aataccatcc tgctttgaaa agatcaggtc aggccagtgt ggttgtgttc agtacggtgg
    17281 ctgtgaagac cggcatgccg tttcatgctt ccattgccgg ggcgaaaggt gcagtggaag
    17341 gtcttgccag atctctggcc gcagaatggg caccttccat ccgggtgaat gtagtggctc
    17401 catctctgac agatacacca ctggctgaac ggttgctgga ttccgacagt aaaagagaag
    17461 cttcagcagc cagacatccc ttgaaacaga tcggggatgc aaaaaacaca gcttcactgg
    17521 taagctggct gttgagtgat gattcggttt tcatgtcagg gcaggtgttg cgaccggacg
    17581 gcggcatctc ttctttgaaa gtatgaacgt gaaaaaacaa gacctgccac aaaagatctg
    17641 tccggtatgt aaccggccgt tcacctggcg aaagaagtgg gaaaagaact gggatgaggt
    17701 gaaatattgc agcgaacgtt gtcgccggca aaaaacaaac tgaacatgct ataccagata
    17761 catacaaaac agttcctgcc gataagtgtc aaagaagcgt gggatttcct gtcatcacct
    17821 gcaaacctcc aggccatcac tcctg
//
LOCUS       DAOMAX010000023        17571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_344_length_17571_cov_4.157285, whole genome shotgun sequence.
ACCESSION   DAOMAX010000023 DAOMAX010000000
VERSION     DAOMAX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17571
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_17571_cov_4.157285"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PK742_02515"
     CDS             <1..549
                     /locus_tag="PK742_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75491.1"
                     /translation="SVIMRYPLKQAGIDTLGSYLYIDVGGGSTEFALLDQGKMVRNRS
                     FNIGTLRILNDQVEETDWKQQMKWLQEVKQDFPKLAAIGVGGNINKVMKMYGKQGRLY
                     LTPALLKKVLAYIEGLSMAERIRDLGLKPDRADVIVPAMRIYVNALKWAGVERIYVPK
                     QGLSDGIITELWEAHRNNARRH"
     gene            complement(551..3409)
                     /locus_tag="PK742_02520"
     CDS             complement(551..3409)
                     /locus_tag="PK742_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU75492.1"
                     /translation="MLRNITALLLSVFIFSYHLDVQAGTLRGQVTDSKTGETLIGVNV
                     FIDGTSTGTATDLDGYYSIEGLDAGTYTLVFSYISYTTYTVTDIELGANETKVIDVLL
                     SELTTELTTVIITARQVDNNATAILTQQKKSINVQDGISAQEIARFGSSDAGESMKKV
                     TGASLVDGKYVYVRGLGDRYSNTQLNGQNLPSTDPYRNSTQLDIIPANLLDNIVTAKT
                     FTPDQPGNFTGGNVNITTKSFPDKFTLGISASAGFNDQSSFQSDFIGMSRGKLDWLGF
                     DDGGRDLPEVLADEDTRPNFIQSFYLTARTNDSLAALLDEGSKAINPNMTPISYTSPV
                     NHGLSFSVGNEFKIRERPLGFILGLNYKRDYTLYTDGTSAQRQLIQFDGPGLNNNFNL
                     TDINGVENPQVGGLLNLAYKVTDNNEIGFNMLYNHDASISAREQYGKYPSVISNSDAI
                     FETRTLSFRERQLTSLQLTGKHVIAKAKDIRIEWSAGQVYSNQLEPDLRFFANSVVGD
                     TSFYINPSEYPQPYHFWRYLNDDQTLGKLDIFIPFLTEKDKGNKIKLGGFYSSKNRDF
                     TEDRWQVVARDGESYNGNPDDYFGPANTGVIGYDDVLNRYIIGNYIVDRTVGGNSYGG
                     NETIIAGYGMFIYQLTPLLKFIGGARAEYTDISVSSADTSKEAGQIQVTDFLPSVNLI
                     YALNENSNLRASFSQTIARPNLRELAPFFSFEFIGGETYLGNPDLERTNITNYDLRWE
                     LFPKPGELIALSAYYKTFINPIIKVYNPTATNPEIQFKNVDEAIVYGLEFEFRKNLSF
                     ISQKLEKFSFGTNLSLIQSRVDLDSLELAVNQSLNPSFSESRPFQGQSPYLVNANLNY
                     FHPDLGLDVTASFNVFGPRLTEVSQAGTPDIYEQPAPALDLFIKKDFAENYSVRIGGK
                     NLLNSKLLKTIEYKDVEYTVQSYTVGRTYSITLSYNIQ"
     gene            complement(3536..5194)
                     /locus_tag="PK742_02525"
     CDS             complement(3536..5194)
                     /locus_tag="PK742_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75493.1"
                     /translation="MKRLQLLTLAALATMGTQAQIVVTDADLVAGGTYTWTTGNTYLL
                     DGFVFLEDGGSLTIQPGVIIKGKETPSSDDLASTLIICQGATIQADGTAEQPIIFTSE
                     LDDLSVANDLSYNDRGLWGGVIILGYAQIGNATETAAIEGIPTEDPRGTYGGNNDADN
                     SGYMHYCSIRHGGAEIGAGNEINGLTLGGVGSGTNFEHIEVYANLDDGIEFFGGSPNC
                     KWMAMAFCGDDSYDWDQGFRGKGQFWFAVQDAASGDCGGEWDGATPDGASPYSNPTIY
                     NATFIGAGTDGSAENGTAMLMRDATGGTVANSIFTSFVNHAIEVEDLPSASGVDSRQR
                     LEDGDLSILNNIYFDFGTGNDFVAGELIRATTDAEDPTCGWLISHLTSNNNTSEDPEL
                     GAISRIPDNGLDPRPSSDGPAYTGVMATEPADAFFSDVDYKGAFEANGPMWLEGWTAL
                     SANNHLVVPVAIQETDLISQISMFPNPVSSAALISLNLENSASLTISIVDMQGRVVNQ
                     IATGQFFAAGQHTVTVNTEALAAGQYMLNISNVNGQVATISFVK"
     gene            complement(5326..5754)
                     /locus_tag="PK742_02530"
     CDS             complement(5326..5754)
                     /locus_tag="PK742_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75494.1"
                     /translation="MAADQILIICDGEFSFGSMLASYIRFYSIDQFQPVVITLHQQHL
                     HPLAVQVMSEDGIDISECKPLNINKIKNNSFRVAVHITSQVQSGIPEYIHAEEVIRLQ
                     YDEPVRSTHYNEVVLAYRQTREAIKTSGIELVGQWQQVLV"
     gene            5875..6837
                     /locus_tag="PK742_02535"
     CDS             5875..6837
                     /locus_tag="PK742_02535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HQU75495.1"
                     /translation="MLQWSTPVNASQAAQCITSEDRLLLTGSCFSSQIGSKLAQSGWH
                     TTINPAGVMYNPLSIAAQINRWIQNKKPEPDELFLHQGLWRHADWHSSFAAATKEAAL
                     ETLADSVSAGRNALQEANVLILTPGTARVYERAADGHLMANNHKLPSSDLNNRILSFR
                     EVKQCLEELFSELTIFRPDLQIILTLSPVRHIRDGLEMNAYSKAILLSAIREVLQHQT
                     SASYFPSYEIMVDELRDYRFYEADLIHPNAVATDHIWNIFQQVFLDTSGRQQVKEVEQ
                     YRSMAAHRILHPDAEESTRFREKLSAIKQALQTRYPHLTFPEQE"
     gene            complement(6841..8280)
                     /gene="rmuC"
                     /locus_tag="PK742_02540"
     CDS             complement(6841..8280)
                     /gene="rmuC"
                     /locus_tag="PK742_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQU75496.1"
                     /translation="MSDHILWILLALLLGILIGWLLHRIFFSQKNVDPSLLEEARTGM
                     IREQEQHQLTRQQLQELQGIRNQLQTRLEELQQETAAIRERLVHQQESLVEKDQSVRK
                     LQEEQTRHQQQFIQQEKENAELRADNRSLQEKLRTFDEELTRKFEVLSQQILDDKSKK
                     FTELNKEQISRVMQPFEKEIKEFKEKVNETYITESKERFKLQGEIEKLMQLNQQLSDD
                     ARNLTRALTVDTKQQGDWGEMILEKILEQSGLVKGREFTMQESMQDAEGNRLRTDVII
                     KLPDERNIIIDSKVSLTDYDRFMKADDADEQEAALQRHVRSVKGHVDILHKKSYADYP
                     GSPEFIFMFIPVEPAFMVAMQGDPELWNYAYQKNIILIGPTNLIAALKLVAEIWKKEY
                     QSRNAMEIADRAGALYDKFAGFTESLLDVGKNLGKAGESYDKALSQLKDGKGNLIGRV
                     EQLRKLGAKNKKQLDQQLLLPDDDIQDDD"
     gene            complement(8369..8445)
                     /locus_tag="PK742_02545"
     tRNA            complement(8369..8445)
                     /locus_tag="PK742_02545"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8409..8411),aa:Met,seq:cat)
     gene            complement(8483..9316)
                     /locus_tag="PK742_02550"
     CDS             complement(8483..9316)
                     /locus_tag="PK742_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU75497.1"
                     /translation="MKLYSIHAGYFKLDGGAMFGVVPKKMWQKLVPADENNLCTWAMR
                     CLLVDTGERKILIDCGMGNKQDEKFMGHYEPHGPTLERSLASHGFQPEDITDLFLTHL
                     HFDHCGGAVKRTEDGQLIPAFPHAKVWSNHQHWDWAIHPNDREKASFLKENLLPLEEH
                     GVLHFLDEGQSLCPEVDTIWVNGHTEAMMLPVIHTGNKTIVYMADLLPAAAHIPLPYI
                     MGYDIRPLMTLEEKKDFLQMALDNNYYLFFEHDAVNECCNLRMTDKGIRPQEFFPLSA
                     L"
     gene            complement(9339..9824)
                     /gene="ribH"
                     /locus_tag="PK742_02555"
     CDS             complement(9339..9824)
                     /gene="ribH"
                     /locus_tag="PK742_02555"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQU75498.1"
                     /translation="MATRDLSSYRADEVPSGKSFRIAVITSDWNEEITHAMEKACLDT
                     LIHHEVVEENISVIHVPGSYELTSAAQMLLETSEQDAVVCIGCIIKGETPHDVYLAQA
                     VSQGLIQVSIDYSTPVLFGVLTTLDLQQAKERAGGRHGNKGVEAAVTALRMAALRRKF
                     I"
     gene            complement(9824..10507)
                     /locus_tag="PK742_02560"
     CDS             complement(9824..10507)
                     /locus_tag="PK742_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75499.1"
                     /translation="MTEQKNAVAEAHDGDVYRQVEQFFLKNRNMILGVLAVLVIGIGG
                     YFGYKKFIQEPKELDARNKITIAQGYFANDSLDLALNGDGINPGFLAISSSYKNTATG
                     NLCQYYIGVIYLKQEQTDLAIEHLGKYKPGTDELKGVTHMLLGHAWADKGDNTKAVAE
                     YKEAGKSAKNDMYSPYYYKMAGDLLLVMEDYGQALDMYKLIKKEYPLSEEGQNIDKEI
                     AYAETKMGK"
     gene            complement(10520..11533)
                     /gene="pdhA"
                     /locus_tag="PK742_02565"
     CDS             complement(10520..11533)
                     /gene="pdhA"
                     /locus_tag="PK742_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HQU75500.1"
                     /translation="MATAVKTDRSKITKETYLEWYEMMYRMRRFEEKAGQLYGMQKIR
                     GFCHLYIGQEAVAAGAITATRKEDPIITAYRDHGLALAKGMSCNEAMAELYGKATGCT
                     KGKGGSMHFFSKEHYFFGGHGIVGAQIPLGAGIAFAEKYKGNDNVTLCFFGDGAVRQG
                     ALHETFNMAMLWKLPVVFIVENNGYAMGTSVERTTNLLDIYKIGMAYDMPSYPVDGMD
                     PESVHMAIHEAAEKARNGEGPTFLEIRTYRYKGHSMSDPQKYRTKEEVEEYKMRDPVE
                     VTRTRIIDKKYASEKELEDMEAAIEQEIADCVEFAENSPFPDDSAVYEDIYLQQDYPF
                     IKD"
     gene            11599..12678
                     /gene="recF"
                     /locus_tag="PK742_02570"
     CDS             11599..12678
                     /gene="recF"
                     /locus_tag="PK742_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HQU75501.1"
                     /translation="MLTRLQLLHYRNHQSAGYSFDNQTVLINGPNGSGKTNLLDAIYF
                     CCMCRSYFLRQDQLLVQHGHDFFRLDASFDNQGQTDEVSVKVVSGRKKEVLLNGVAYE
                     KLADHIGQFPVIMITPDDTRLIQQYADERRRFLDLTISQVDREYLFDLQQYQKVLAQR
                     NAWLKQAWQRGADKEVLDSLDKQLWPAAGRIWQARRNFTERIVPGIQAIYARLSGNRE
                     VPGIDYQSDLNHADAAELHARTRQEDLAAGRTQAGVHRDDLLFTLDGEPLKQVASQGQ
                     IKSLLIALKLAAFHGDPDGRKPLLLLDDIFEKLDRKRMEALFSVISEGSWGGMFLTDA
                     DSSRSQDILRNQQVLFDRVVTNNPD"
     gene            12706..12999
                     /locus_tag="PK742_02575"
     CDS             12706..12999
                     /locus_tag="PK742_02575"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQU75502.1"
                     /translation="MPRKSVEQGIGEVIKDLFKAYHIDDKVYAIRIGDVWEEVMGKTV
                     ARYTTGIRLEKDVLHIRIDSAPLRQELFFHRDDIRQRINQAFAEEVVREVHIR"
     gene            13061..13843
                     /locus_tag="PK742_02580"
     CDS             13061..13843
                     /locus_tag="PK742_02580"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF008877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HQU75503.1"
                     /translation="MNEILDKPMVQDYTQYTPEHFKVWEILFDRQMALLKDRAAQVFM
                     DGIEAIHFSAGTIPRFDEFNIRLDTLTGWNAVVVPGLIGNKDFFELLLNRKFPASTWL
                     RTMEQLDYLEEPDMFHDVFGHLPLLTNKTYCAYLYGLSKIALSYIDNPVAVELISRIY
                     WYTVEFGLIRDNGLLRIYGSGILSSTGESVYCLKSGIPSRRIDYQVEKILDTPYIKDK
                     FQEQYFVIESCLELFESLPDIEQGIRKRLEHPELYVKGPDED"
     gene            13904..15175
                     /locus_tag="PK742_02585"
     CDS             13904..15175
                     /locus_tag="PK742_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQU75504.1"
                     /translation="MSTVKDMPLAERLRPKDLDTFAGQQHLVGKGQVLRRMYEQGQVT
                     SMIFWGPPGTGKTTLANILAAKAGLPFVSLSAVTAGVKDVKETIQLAQKSGRILLFID
                     EIHRFNKAQQDSLLGAVEKGLITLIGATTENPSFEVIRALLSRCQVYVLEPFTDQDLA
                     QLLQQAVEKDAWLLEKKVHLEETTALLRLSGGDGRKLLNLLELVVQTVGTIPVVVTDQ
                     LVEEVVHSRMAAYDKTGEMHYDLVSAFIKSMRGSDPNAAMYYLARMLEGGEDPKFIAR
                     RMLILASEDIGNANPNALLLATSCFQAVTMLGYPECMLTLSQTAIYLAASVKSNAAYT
                     ALQKARKLAREQGDQPVPLALRNAPTALMKDMQYGKDYQYAHDFTGNFTHMEFLPEAL
                     SGTVLYEPGMNPREEEMRKRLRELWKSKYGY"
     gene            15188..15490
                     /locus_tag="PK742_02590"
     CDS             15188..15490
                     /locus_tag="PK742_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HQU75505.1"
                     /translation="MKRPDEDKMWEMAEKRVAFRRHLMVYILVNLLLVILWYFTSYKH
                     NNGDGYWFAYPLLGWGIGLAFHYWGAYHDDFDSVEKEYQKIREKYNSADQDNDVVS"
     gene            15709..15978
                     /locus_tag="PK742_02595"
     CDS             15709..15978
                     /locus_tag="PK742_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQU75506.1"
                     /translation="MSIQPLADRVVIKPAPAETKTASGLVIPDTAKEKPQQGTVMAVG
                     PGKKDEPTTLKVGDTVLYGKYSGTEISVDGDDYLIMRESDVFAVI"
     gene            16000..>17571
                     /gene="groL"
                     /locus_tag="PK742_02600"
     CDS             16000..>17571
                     /gene="groL"
                     /locus_tag="PK742_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616617.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQU75507.1"
                     /translation="MMAKLIHFNSDAREALKIGVDTLANAVKATLGPKGRNVVIEKKY
                     GAPNVTKDGVSVAKEIELEDPVQNMGAQMVREVASKTSDVAGDGTTTATVLAQAIMTA
                     GLKNVAAGANPMDLKRGIDKAVEAIVANLKKQSKKVGDDNSKIEQVGTISANNDPEIG
                     KLIAEAMAKVSKDGVITVEEAKGTDTYVDVVEGMQFDRGYLSPYFVTNADKMEAEMER
                     PYILIYDKKISTMKDILPVLEKVAQQGAQLLIIAEDVDGEALATLVVNKIRGSLKVVA
                     VKAPGFGDRRKEMLEDIAVLTGGTVISEERGYKLENADLTFLGRAETITVDKDNTTIV
                     NGSGKKEGIKARINQIKGQIENTTSDYDREKLQERLAKLSGGVAVLYVGAATEVAMKE
                     KKDRVDDALHATRAAVEEGIVPGGGVAYIRASAVLDKLELDNEDEKIGVEIVRKALEA
                     PIRTIVDNAGIEGSIVVQKVRDGKADFGFNARTETYENLLAAGVIDPVKVTRVALENA
                     ASIAGLLLTTEVTIVD"
BASE COUNT         4471 a         4161 c         4373 g         4566 t
ORIGIN      
        1 tctgtcatca tgcgttaccc gttgaaacag gcgggaattg atacactggg tagctacctc
       61 tacatcgatg tgggtggcgg aagcaccgag tttgccctgc ttgatcaggg gaaaatggtc
      121 cgtaaccggt cgtttaatat tggtacacta cgtatattaa atgatcaggt agaagagact
      181 gactggaagc aacagatgaa atggctgcag gaggtcaaac aggattttcc taaacttgct
      241 gccataggag tagggggtaa tatcaacaag gtgatgaaga tgtatggtaa gcagggaaga
      301 ctgtatctga ctcctgcctt attgaaaaaa gtcctggcat atattgaggg gttatccatg
      361 gcagagcgca tccgcgatct gggcctcaag ccagaccggg ctgatgtcat tgtacctgcc
      421 atgcgcatct atgtcaatgc acttaaatgg gctggtgtag agcgaatcta tgtgcccaaa
      481 caggggcttt ctgatggcat catcacagaa ctgtgggaag cacacagaaa caacgcccga
      541 aggcactgat ctactgaata ttgtaagaca gcgtgatcga ataggttctt ccaactgtgt
      601 aggactgtac cgtgtactct acatccttgt actcaatggt cttgagaagc ttgctgttca
      661 gcaggttttt ccccccaatt cgcacagagt agttctctgc aaagtctttc ttgatgaaca
      721 gatccagagc tggggcaggc tgttcataga tatcaggagt acctgcctga cttacctctg
      781 tcaatcttgg tccgaaaaca ttgaagctgg ccgttacatc caggccaaga tccggatgga
      841 aatagttcaa gtttgcgttg accagatatg gcgactggcc ctggaatgga cgggattcgc
      901 tgaaggaagg attcagactt tgattgaccg caagttccag gctgtccagg tccacgcgac
      961 tttggatcag gctgaggtta gtgccgaaac tgaatttttc cagcttctgt gaaatgaaag
     1021 aaaggttctt tctgaactca aattccagtc catatacaat ggcttcatca acattcttga
     1081 actggatctc cgggttcgtt gctgtaggat tatatacttt gatgatcggg ttgatgaagg
     1141 tcttgtaata tgcactcagg gctatcaatt cgcctggctt ggggaataat tcccagcgca
     1201 ggtcatagtt ggtgatattg gttctttcca gatccggatt gcccagatag gtctctccac
     1261 ctatgaactc aaaggagaaa aatggagcaa gttcacgcag attgggtctt gcaatggtct
     1321 ggctgaatga agcccggagg ttcgagtttt cattcaatgc atagatcagg ttgactgacg
     1381 gaagaaagtc tgttacctgt atctggcctg cttctttgct ggtgtctgca ctggatacac
     1441 tgatgtcagt gtattctgct cttgctcctc caatgaactt aagtagtggg gtcagctggt
     1501 aaatgaacat gccatatccg gcaatgatgg tttcgttacc tccgtagctg tttccgccaa
     1561 cggtacggtc cactatataa ttgcctatga tatatctgtt cagtacatcg tcatatccga
     1621 tcactccagt gttggcagga ccgaaatagt cgtcggggtt accgttgtat gattcgccgt
     1681 cacgggcaac tacctgccag cggtcttcgg taaagtcgcg gttcttactg ctgtagaatc
     1741 cgcccagttt gatcttatta cccttatctt tttctgtcag gaaaggaata aagatatcca
     1801 gtttacccaa tgtctgatca tcattgaggt atcgccagaa gtgatacggt tgagggtatt
     1861 ctgatgggtt tatataaaat gaagtatcac ctactactga gttggcaaag aaacgcaggt
     1921 ctggttccag ctggttggag tacacttgtc cggcagacca ttctatccga atgtctttgg
     1981 ctttggctat cacatgtttg ccggttagtt gcagtgatgt gagctgacgt tcacggaaag
     2041 acaaggttct tgtttcaaaa atggcatctg agttgctgat cacactcggg tatttgccat
     2101 actgttcacg tgcgctgatg gatgcatcgt ggttgtacag catattgaat ccaatttcat
     2161 tgttatcggt caccttgtat gccagattca gaagtccgcc cacctgaggg ttttccactc
     2221 cgttgatgtc tgtcagattg aaattgttgt tcagcccggg gccatcaaac tggatcagct
     2281 gcctctgagc agatgtgcca tctgtgtaga gtgtataatc acgtttgtaa ttcaagccca
     2341 gaataaaacc caatggcctt tccctgatct tgaattcatt tcccactgaa aaggataaac
     2401 cgtggttaac cggcgatgta taggagatcg gggtcatatt cgggttaata gctttggatc
     2461 cttcatccag gagggctgcc agtgaatcgt tggtgcgtgc agtcagatag aaactctgaa
     2521 tgaaattagg acgggtatct tcatctgcca gtacctcggg cagatcgcgg ccgccatcat
     2581 caaagccaag ccagtccagt tttccgcggc tcatgccaat gaagtcactc tggaatgaac
     2641 tctggtcatt gaatccagct gaagcagata taccaagtgt gaatttatca gggaacgact
     2701 tggtggtgat attgacatta cctcccgtaa aattgccggg ctgatcaggg gtgaaggtct
     2761 ttgcggtaac aatattgtca agcagatttg caggaatgat atccagttgt gtgctgttgc
     2821 ggtatggatc tgtagatggt agattctgcc catttaactg ggtgttggaa tatctgtcgc
     2881 caagtcctct tacatagacg tacttgccat ccaccagtga ggcaccggta acctttttca
     2941 tgctttcgcc ggcgtcagaa ctaccaaacc tggcgatctc ctgtgcggag ataccatctt
     3001 gtacattgat gctttttttc tgttgtgtga gaattgcagt ggcattatta tccacctgcc
     3061 tggctgtgat gatcacagtg gtcaactcag tggtaagctc agacaacagc acatcaatta
     3121 ccttggtctc atttgctccc agttcaatgt ctgtaacagt ataggtagta taggagatat
     3181 aggaaaatac aagggtatag gtgcccgcat ccaatccttc tatactataa tatccatcca
     3241 gatctgtggc ggtaccggta gaggtgccat caatgaacac attcacaccg atcagggtct
     3301 ctccggtctt ggagtcagtg acctgtccac gcagggtgcc tgcctgaaca tccaggtgat
     3361 aactaaatat gaatacagac aggaggagag cggtaatgtt gcgcagcata cccaaatttt
     3421 ttgcaaagct accagcacat cattaccagc cggttaatgg ctcgtaaact taacattaac
     3481 taaacataaa agggctgtca tatgacagcc ctttctatcc atccggataa accgattact
     3541 tcacaaaaga gatggtagca acctgaccat ttacattact gatgttcagc atatactgac
     3601 cggctgcaag tgcctcagta tttactgtta cagtatgctg tccggctgca aagaactgac
     3661 ctgttgcgat ctggttgacc acacgaccct gcatatcaac aatgctgatt gtaagggaag
     3721 cactgttttc caggttcagg cttatcagcg cagcagagct aaccgggtta gggaacatac
     3781 tgatctggct gatcaggtct gtttcctgaa tggccacagg cactaccagg tgattattgg
     3841 cagataatgc tgtccagcct tccagccaca tagggccatt ggcttcaaat gcacctttgt
     3901 agtccacatc agagaagaag gcgtctgcag gctctgtagc catcacaccg gtataggcag
     3961 ggccatcaga agaagggcgg ggatcaaggc cgttgtcagg aatcctgctg atagctccca
     4021 gttcaggatc ttcagaagta ttgttgttgc tggtcaggtg agagatcagc cagccacatg
     4081 ttggatcttc cgcatcggtg gtagcacgga tcagctcacc agcaacgaaa tcattgcctg
     4141 tgccgaagtc aaagtaaatg ttattgagga tgctcagatc gccgtcttcc agacgctgac
     4201 gactgtcaac acctgaagct gaaggcaggt cttccacttc aattgcatga ttcacaaagg
     4261 aggtgaaaat gctgttggct actgtaccac cggtggcatc acgcatcagc atagctgtac
     4321 cgttttcggc agaaccgtcg gtacctgcgc ctatgaaagt ggcattgtag atggtagggt
     4381 tggaataggg gctggctcca tcaggagttg caccatccca ttctccgcca caatcgcctg
     4441 aggctgcatc ctgcacagca aaccagaact gacctttgcc acggaagccc tgatcccagt
     4501 cataggaatc atcgccgcag aatgccatag ccatccactt acagttagga gagcctccaa
     4561 agaattcaat accgtcatcc aggttggcat acacttcaat gtgttcaaag ttggtgccgc
     4621 taccaacgcc acccagggta agtccgttga tctcgttacc ggcaccgatc tctgcaccac
     4681 catgacgaat ggagcaatag tgcatgtaac cactgttatc tgcgtcatta ttaccgccat
     4741 aagtaccgcg agggtcttct gtaggaatac cttctatagc ggcggtctcg gtagcatttc
     4801 cgatctgtgc atatccaaga atgatcacac cgccccaaag gccgcggtca ttatagctca
     4861 ggtcatttgc cacgctcaga tcgtccagtt ctgaggtgaa tatgataggc tgctctgcag
     4921 taccatctgc ctgaatggtg gctccctgac agatgatcag tgtagaagcc agatcatcag
     4981 atgagggtgt ttcctttcct ttgatgatca cacccggctg aatggtcagg ctgccgccat
     5041 cttccaggaa tacgaagccg tccagtaaat acgtgtttcc ggtggtccag gtataggtgc
     5101 cgccggctac caggtctgca tcggtaacaa ctatctgggc ttgtgtaccc attgttgcca
     5161 aagcagccag agtgagaagt tgaagtcttt tcattgcgct attaatttga gcgcaaagga
     5221 accagtgcga gtttacgttg gaattattac accgttaagt aatggttaac ggtcaataat
     5281 attaagataa ggatacctga ggttaacatc tgcttaataa tgacattaca caagcacctg
     5341 ttgccactga cctaccagtt caattcctga tgttttgata gcctcacggg tttgcctgta
     5401 ggcaagtaca acttcattgt aatgggttga tctgacaggt tcatcatact gcagcctgat
     5461 cacttcttct gcgtggatgt attctggaat accggattgc acttgcgagg tgatgtgcac
     5521 tgcaacccga aaggagttat tttttatttt gttgatgttc agcggcttac attcactgat
     5581 gtcaatgccg tcttcactca tgacctggac agcaagcgga tgcaggtgtt gctgatgcag
     5641 ggtgatcaca acaggctgaa actgatcaat agaatagaat cgaatataag aagcaagcat
     5701 gcttccaaag ctgaactcac cgtcgcaaat aatgagtatc tgatcagctg ccatagtaga
     5761 aacggacggt aatcgaaaaa agattatttc ctgtatctaa agataaagtg agtcaaaggt
     5821 aaattcaaac ctctttcgaa tttttatatt cacagcctca attgaattgt tgatatgtta
     5881 caatggtcca ctcctgtgaa tgcatctcag gcggcacaat gcattacttc tgaagaccgg
     5941 ctgctgctta caggcagttg cttcagttct cagatcggca gcaaactggc acaaagtggc
     6001 tggcatacaa ccatcaatcc tgccggggtg atgtataatc cactcagcat agctgctcag
     6061 atcaaccggt ggatacagaa caagaagccg gaaccagatg agttgtttct gcatcaggga
     6121 ttgtggcgtc atgcagactg gcattcatcc tttgccgcgg ccacaaaaga agcagctctt
     6181 gagactcttg ctgattcagt cagcgcagga cggaatgcgt tgcaggaagc caatgtactg
     6241 atactcactc ccggcacagc cagggtgtat gaaagggccg ccgatggaca cctgatggcc
     6301 aacaaccaca aactaccatc aagtgatctg aacaaccgga tcttatcgtt ccgggaagtg
     6361 aaacaatgtc tggaagaact gttcagtgag ctgaccatat tcaggccgga ccttcaaatc
     6421 atcttgaccc tgagtccggt caggcatatt cgggatggcc tggagatgaa cgcctacagc
     6481 aaggcgatac tgctatctgc tatcagggaa gtgttgcaac accagacaag cgcatcctat
     6541 tttccatctt atgagatcat ggtggatgaa ttacgtgact accgcttcta tgaggcagat
     6601 ctgatccacc cgaatgcagt tgccacagac catatctgga acatctttca gcaagtgttt
     6661 ctggatacaa gcggaaggca gcaggtaaag gaagtagagc aataccgttc aatggctgca
     6721 cacaggatac tgcatccgga tgcggaagaa tcaacaaggt tcagggaaaa actgtcggca
     6781 ataaaacagg cactacagac cagatatcct catctgacct ttccggaaca ggaatgaacc
     6841 ttagtcatca tcctgtatgt catcatcagg aaggagcaat tgctggtcga gctgtttctt
     6901 attcttcgca cctagcttcc gaagctgttc tacccgtccg atcagatttc ctttgccatc
     6961 cttcagctgg ctcagggctt tgtcgtaact ctcgccggct ttacccagat tcttgcccac
     7021 atcgagcagg ctttccgtga acccggcaaa tttgtcatac aaggcacccg cacgatctgc
     7081 gatctccatg gcatttctgc tctggtattc tttcttccag atctctgcta ccagcttcaa
     7141 tgctgctatc aggttggttg gacctatcag gatgatattt ttctgatatg cgtagttcca
     7201 gagttcggga tcgccttgca tggccaccat gaaagcgggc tccaccggaa tgaacatgaa
     7261 gatgaactcc ggactgccgg gataatcagc atagcttttc ttgtggagga tgtccacatg
     7321 tccttttaca cttctgacat gtctctgcaa agctgcttcc tgttcatctg catcatcagc
     7381 tttcatgaac cggtcataat cggtcaatga cactttgcta tcgatgatga tgttgcgttc
     7441 atctggaagt ttgatgatca catccgtgcg cagtctgttt ccttctgcat cctgcatgct
     7501 ttcctgcatg gtgaattccc ggcctttgac cagtccgctc tgttccagta tcttttccag
     7561 gatcatttcg ccccagtcgc cctgctgctt ggtatcaacc gtcagtgcac gggtcaggtt
     7621 gcgtgcatca tcgctcagct gctggttgag ctgcatcagc ttttctatct ctccctgcag
     7681 tttgaagcgt tctttggatt ctgttatata ggtttcattc accttctctt tgaactcttt
     7741 gatctctttt tcaaaaggct gcatcaccct ggatatctgt tctttgttga gctcggtgaa
     7801 ttttttgctt ttgtcgtcca ggatctgctg gctgagtact tcaaattttc ttgtcagttc
     7861 ttcatcaaag gttcgcagtt tttcctgcag tgaccggtta tcggcacgaa gctctgcatt
     7921 ctctttttcc tgctgaatga actgttgctg atgtctggtc tgttcctcct gtaatttcct
     7981 gacagactgg tctttttcga caagggattc ctgctgatga acaaggcgct cgcgaatggc
     8041 agctgtttcc tgttgtaatt cttccagtcg tgtctgcagc tggttgcgaa taccttgcaa
     8101 ttcctgcagc tgttgcctgg tcagctggtg ttgttcctgt tcgcgaatca tgccggttct
     8161 ggcttcttcc agaagagaag ggtccacatt tttttggctg aaaaaaatac gatgaagcag
     8221 ccacccgatg aggatgccta aaagtaaggc cagaagtatc cagagtatat ggtcagacat
     8281 accgtgtgaa gttacaaatt agagattttc ataccggctc aaaggatggg cggaaaaaaa
     8341 aatccagaca caaggtctgg actggttatg gtagcgggga cgagaattga actcgtgacc
     8401 tccgggttat gaatccgacg ctctaaccat ctgagctacc ccgccaaaag gtggttgcaa
     8461 aggtagtttt ttccaacatg gattacaatg cactgagcgg gaaaaattcc tgcggccgaa
     8521 tgcctttatc ggtcatccga aggttgcagc attcattcac tgcatcgtgt tcgaaaaaga
     8581 gatagtaatt gttgtccaat gccatttgga gaaagtcttt tttctcttcc agtgtcatca
     8641 gtggacgaat atcatacccc atgatatagg gcagtggaat atgggcagct gcaggtagca
     8701 ggtcggccat atagacaatg gtcttgttgc cggtatgaat aacaggcagc atcatggctt
     8761 ctgtatggcc attcacccag atggtatcta cttcagggca aagcgactgg ccctcatcca
     8821 gaaaatgcag cacgccatgc tcttcgagcg gcagcagatt ttctttcaga aaggaggctt
     8881 tttcccggtc attaggatgt attgcccagt cccagtgttg gtggttactc cagacttttg
     8941 catgcggaaa ggcaggaatc agttggccat cttccgttct tttgaccgct ccaccacaat
     9001 gatcaaagtg gaggtgggtc aggaagaggt cggtgatgtc ttccggttgg aaaccatgac
     9061 tagccagcga tctttccagt gttggtccat gcggctcata gtgtcccatg aatttctcat
     9121 cctgcttatt acccatgcca cagtcgatca ggatctttcg ttcgccggta tccaccagga
     9181 gacatctcat ggcccaggta cacaggttgt tttcatctgc aggcaccagt ttctgccaca
     9241 tcttcttcgg caccacgcca aacatagcgc caccatccag cttgaaatag ccggcatgaa
     9301 tggaatatag tttcatgcct taaaggtagg aaatgagatc agatgaattt tcttctgagt
     9361 gcggccatgc gcagcgcagt cacagcagct tctacgcctt tattgccatg ccggccaccg
     9421 gcacgttctt ttgcctgctg aaggtccagt gtggtgagaa caccaaacaa aacaggtgta
     9481 ctgtaatcga tgctgacctg gatgagtccc tgagatacgg cctgagcaag atagacatca
     9541 tgcggcgttt ctcctttgat gatacaacct atacagacca ctgcgtcctg ctcactggtt
     9601 tccagcagca tctgtgcagc agaggtgagt tcataacttc cgggcacgtg aatcacactg
     9661 atattctctt ccacgacctc atgatggatc aatgtatcca ggcaggcttt ttccatcgca
     9721 tgggtgatct cttcattcca gtcggaagtg atcactgcaa tccggaaact cttacctgag
     9781 ggaacctcgt ctgcccggta ggaagacagg tcgcgggtag ccattatttg cccattttgg
     9841 tttcggcgta agcgatctct ttgtcaatat tctgaccttc ttcgctcaat ggatattctt
     9901 tcttgatgag cttgtacata tccagtgcct gaccataatc ttccatgacc agcaacagat
     9961 cgccggccat cttgtagtag taggggctgt acatgtcatt tttggcagac ttacctgctt
    10021 ccttatattc cgcaaccgcc ttggtattat cacctttatc agcccaggca tgacccaata
    10081 gcatatgggt tacgcctttc agttcatcgg taccgggttt gtacttgccc aggtgttcaa
    10141 tggccagatc tgtttgttcc tgtttcaggt agatgacgcc aatatagtac tggcaaagat
    10201 tgccggtagc tgtattcttg taactggagc tgatagccag gaaaccgggg ttgataccat
    10261 ctccgttcag ggccaggtcg agagagtcat tggcaaaata gccctgggct atggtgatct
    10321 tgtttcgggc atccagttct ttaggttcct ggatgaattt cttgtagcca aagtagcctc
    10381 caataccgat caccagcaca gccagtacac ccaggatcat gtttctgttc ttcaggaaga
    10441 actgctccac ctggcggtaa acgtcgccgt catgtgcttc ggccacagca tttttctgtt
    10501 cagtcatgta taaaaaatat taatctttga taaatggata gtcctgctgc agatagatgt
    10561 cttcatatac tgctgaatca tcagggaagg gggagttctc agcaaactcc acgcagtctg
    10621 cgatctcctg ctcaatggca gcttccatat cttccagctc tttctctgaa gcgtatttct
    10681 tgtcaatgat ccgtgtccgg gtcacttcaa ccgggtcacg catcttatac tcttctactt
    10741 cctccttggt gcggtacttc tgcggatcac tcatagagtg tcctttgtaa cggtaggttc
    10801 tgatctccag gaaagtagga ccttcaccat tccgtgcttt ttcagctgct tcatgaatgg
    10861 ccatgtgtac actttccgga tccatgccat caacggggta ggagggcatg tcataggcca
    10921 tgccgatctt ataaatgtcc agcaggttgg tggtgcgttc tacggaagtt cccatagcat
    10981 agccattgtt ctcaacaatg aataccaccg gcagtttcca tagcatggcc atattaaagg
    11041 tctcatgcag agcgccctgt ctgacagcgc catcgccaaa gaagcacagg gtcacattgt
    11101 cattcccctt atatttttca gcaaatgcaa tgcctgctcc caatggtatc tgagcaccta
    11161 cgataccatg acctccaaag aaatagtgtt ccttgctgaa gaagtgcatg gaacctccct
    11221 tgcctttggt gcaccctgtg gctttgccat agagttcagc catcgcctca ttgcagctca
    11281 tgcctttggc cagggccagc ccatggtcac ggtatgcggt gatgatgggg tcttccttgc
    11341 gggtagcggt aatggcccct gcagcaacag cttcctgccc tatatacaaa tggcagaaac
    11401 cgcgtatctt ctgcatgccg taaagctgcc cggccttctc ctcgaaacga cgcatccggt
    11461 acatcatctc ataccattcg aggtaggttt cttttgttat ttttgaccgg tcggttttca
    11521 ctgctgttgc catgctctcc acgggtttaa aaagattggc aaagttaaga aacaagtcac
    11581 ataataaaaa ccaccgcatt gcttacccgg ctacaactgt tgcattaccg gaaccaccag
    11641 tctgcgggct attcctttga caatcagacc gtactgataa acggccccaa cggttccggg
    11701 aaaacgaacc tgctggacgc catctatttc tgttgcatgt gcagaagtta tttcctgcgg
    11761 caagaccagt tgctggtgca gcatggtcat gatttctttc gcctggacgc ctcatttgat
    11821 aaccagggtc agacagacga ggttagtgtc aaggtggtta gcggcagaaa aaaggaggtg
    11881 ctgcttaacg gtgtggccta tgagaaactg gctgatcaca tcgggcagtt tccggtcatc
    11941 atgatcaccc ctgatgatac ccgcctgatt cagcaatatg ctgatgaacg acggcgtttt
    12001 ctggatctga ccatctcgca ggttgaccgt gaatatctgt ttgatctgca acagtatcag
    12061 aaagtacttg cacagcgcaa cgcctggcta aaacaggcct ggcaaagggg ggctgacaag
    12121 gaagtgctgg attcactgga taaacaactg tggccggcag ccggccggat ctggcaggcc
    12181 aggcggaatt tcacagaacg catcgttccg ggtattcagg ctatctatgc ccgtttgtca
    12241 ggaaaccgtg aagtacctgg catcgattat cagagcgacc tgaatcatgc agatgcagca
    12301 gaactccatg cccgcacaag gcaggaagac cttgcagccg gccgcactca ggcaggtgtg
    12361 cacagagatg acctgctgtt caccttagat ggagaacccc tgaaacaagt ggccagccag
    12421 ggccagatca aatcattgct cattgccctg aaactggcgg catttcatgg tgacccggac
    12481 ggccggaagc cattactgct gctcgatgat atttttgaaa aactggacag gaagcggatg
    12541 gaagcgctgt tcagtgtgat cagcgaaggc tcctggggag gtatgttcct gactgatgca
    12601 gacagcagca ggtcgcagga tatattgcgc aaccagcaag tgctatttga ccgggtggtg
    12661 accaataacc cggattgatt cacgttcata tactaccttc gtcttatgcc acgaaagagt
    12721 gtggaacaag gtatcggcga ggtcatcaaa gacctgttca aagcgtatca cattgatgat
    12781 aaggtctatg ccatccgcat tggagatgta tgggaggagg tgatgggtaa aacagttgca
    12841 cgttacacta caggcatcag gctggagaaa gatgtactcc acatccggat agactctgcg
    12901 cctttgcgcc aggagttgtt ttttcaccgg gatgatatcc ggcaaaggat caaccaggct
    12961 ttcgctgaag aggtggttcg tgaagtccac atccggtgaa caaatcgcgg tcgttcagcg
    13021 ggtgaatatt ctgtaattta gagatcaaat tgtaacacac atgaatgaga tactggataa
    13081 gccaatggtg caggattaca cccaatatac accggagcat tttaaggtgt gggagatcct
    13141 gttcgaccgg cagatggcgt tactgaaaga cagggcagca caggtattca tggacggtat
    13201 cgaagccatt catttttcgg caggtaccat ccccagattt gatgaattca atatcaggct
    13261 ggacaccctg actggatgga acgctgtagt ggttccgggg cttataggca ataaagactt
    13321 ctttgagttg ctgctgaaca gaaaatttcc ggcatctacc tggcttcgca ccatggagca
    13381 actggattac ctggaagaac cggatatgtt tcacgatgtg ttcggccatc tgccactgct
    13441 caccaacaag acctattgtg cctacctgta cggactaagc aagatcgctc tgagctatat
    13501 agataatcct gtagcagtgg aacttatcag tcgcatatac tggtacaccg tagagtttgg
    13561 cctgatcaga gataatggtc tgcttcgcat ttatggttcc ggtatccttt catcaacagg
    13621 agaatctgtg tattgcctga aatcaggaat cccctccagg cgcatagact atcaggtaga
    13681 aaagatactg gatacacctt atatcaaaga caagtttcag gaacagtatt ttgtgataga
    13741 atcctgcctg gaattgtttg aaagtcttcc tgatattgaa caaggcatca ggaagcgtct
    13801 ggaacatcct gagttgtatg tgaaagggcc tgacgaagac tgacaggtta actaacttgg
    13861 caaccggagg tttgtgtctt acaggtctcc ggttttttat gacatgagca cagtgaaaga
    13921 tatgccactt gctgagcggc tccgtccgaa agatctggac acctttgccg gccagcagca
    13981 tctggttgga aaaggccagg tgttgcgccg tatgtacgag cagggccagg ttaccagtat
    14041 gattttctgg gggccgccgg gtaccggtaa aaccaccctg gccaatatac tggctgccaa
    14101 agcaggcttg ccatttgtaa gcctcagcgc tgttacagca ggtgttaagg acgtcaagga
    14161 aaccattcaa ctggctcaga aaagcggtcg cattcttctc tttatcgatg agattcaccg
    14221 cttcaacaaa gcgcaacagg attccctctt gggtgccgtg gaaaaaggac tcatcaccct
    14281 gataggagct accactgaaa acccttcctt tgaggtgata cgggccttat tgagtcgctg
    14341 tcaggtgtat gtgctggaac cctttacaga tcaggatctc gctcagttgc tgcaacaggc
    14401 ggtagaaaaa gatgcctggt tgctggaaaa aaaagtacat ctggaagaga ccactgcatt
    14461 gcttcggttg tctggcggag atggcagaaa acttctgaat ctgctggagc tggtagtcca
    14521 gaccgttggc acgataccgg ttgtagtcac tgaccagctg gtggaagaag tggtgcacag
    14581 tcgcatggca gcttatgaca agacaggaga gatgcactat gacctggtgt ctgcctttat
    14641 caaatccatg cggggaagtg atccgaatgc agccatgtac tatctggccc gcatgctgga
    14701 aggaggcgaa gacccgaaat tcatagccag acgtatgctg atcctggcca gtgaagacat
    14761 tggtaatgct aacccgaatg ccctcttgct ggctacttcc tgctttcagg ctgtaactat
    14821 gctgggttat cctgaatgta tgctcacctt atctcaaact gctatttatc tggcagcttc
    14881 cgtcaaaagc aacgctgctt acacggcctt gcaaaaagcc agaaagctgg ctcgggagca
    14941 aggtgatcaa ccggtaccgc tcgctttgcg caatgcaccc acggcgctca tgaaagatat
    15001 gcagtatggc aaagattatc agtatgccca tgattttaca ggtaacttta cacatatgga
    15061 atttctgccc gaagcattgt cgggaacagt gctttatgag cccggaatga acccaagaga
    15121 ggaagaaatg cgtaaaaggt taagggagct ctggaaatca aaatacggct actaaataat
    15181 caatactatg aaaagacctg acgaagataa aatgtgggaa atggccgaga aacgggttgc
    15241 ctttcgccgg cacctgatgg tctatatact ggttaatctg ctgcttgtaa tcctttggta
    15301 ttttacttcc tataagcaca acaacggaga tggttactgg tttgcctatc ctttgctggg
    15361 atggggaata ggcctggcct ttcattactg gggcgcctac catgatgact ttgattccgt
    15421 agaaaaggaa taccagaaga tcagagaaaa atacaactct gcagaccagg ataatgatgt
    15481 cgtttcctga gcattgtttg caaggttttc ttcctgctgc tgtcattacg gcgcatgcag
    15541 ccgcaactgt ttcctgtcaa ttggatgggt aagaagttgc atcccgcttc actctgacgt
    15601 gatttctgac aaaatgacac aattatttct gtggcacagt ttctgaccac gggcagtcat
    15661 ctgtataaca cttaaaacca aataccagat atgtctaaga aagcgaaaat ctccatccag
    15721 ccgctggctg accgggtggt gatcaaaccg gctcctgctg aaaccaagac agctagtggc
    15781 ctggtcattc ctgacaccgc taaagaaaaa ccacagcagg gtactgtaat ggccgttggc
    15841 cccgggaaga aagatgaacc taccacgctg aaagtaggtg ataccgtgct ttatggaaag
    15901 tattccggaa cagagatcag tgtggacggc gatgactacc tgatcatgcg ggagagtgat
    15961 gtatttgcag taatctgaat ccaaaaaaaa caataagcaa tcatggcaaa actgattcat
    16021 ttcaatagcg atgcaagaga agcattgaag atcggggtag ataccctggc caatgcagtg
    16081 aaggccaccc tgggtcctaa aggacggaac gtggtcatag agaagaaata cggtgcacca
    16141 aacgtaacca aagatggtgt gagtgtggcc aaagagatcg aactggaaga ccccgtgcag
    16201 aacatgggtg cccagatggt tcgcgaagtg gcttccaaga ccagtgatgt ggccggcgat
    16261 ggtaccacca ctgcaacagt actggcacag gccatcatga ccgccggtct gaagaacgtg
    16321 gccgccggtg ccaacccgat ggacctgaag cgtggtatcg ataaggctgt agaagccatc
    16381 gttgccaacc tgaagaagca gtctaaaaaa gtaggtgacg acaatagcaa gatcgaacag
    16441 gtaggaacca tttctgccaa caacgatccg gagatcggta agctgatcgc tgaagccatg
    16501 gccaaagtga gcaaagacgg cgtcattacc gttgaagaag caaaaggaac agacacttat
    16561 gtggatgtgg tggaaggtat gcagttcgat cgtggttacc tgtcgcctta cttcgtaacg
    16621 aatgcagata agatggaagc cgaaatggag cgtccatata tcctgatcta cgacaagaag
    16681 atcagtacta tgaaagacat ccttccggtt ctggagaaag ttgcccagca aggtgcacaa
    16741 ctcctgatca tcgcagaaga tgtggatggt gaagcactcg ctaccctggt ggtgaacaag
    16801 atccgtggca gcctgaaggt agtagccgtt aaagctcccg gctttggcga tcgccgcaag
    16861 gaaatgctgg aagatattgc tgtgctcacc ggtggtaccg tgatcagcga agagcgtgga
    16921 tataaactgg agaatgccga cctgaccttc ctcggtcgtg ccgaaaccat cactgtggat
    16981 aaggataaca ccaccatcgt gaacggctcg ggtaagaaag aaggcatcaa agcccgcatc
    17041 aaccagatca aaggtcagat cgagaacacc acttctgatt atgaccgtga gaagctgcag
    17101 gagcgtctgg caaaactgtc aggcggggtg gctgtactct atgtaggtgc tgctactgaa
    17161 gtagccatga aggaaaagaa agaccgtgtg gatgatgccc tgcatgctac ccgtgctgcg
    17221 gtggaagaag gtatcgtgcc cggtggtggg gttgcctata tccgtgccag cgctgtcctg
    17281 gataaactgg aactggacaa tgaagacgag aagatcggtg tggagatcgt gcggaaggca
    17341 ctggaagctc ctatccgtac catcgttgac aatgccggta tagaaggcag catcgtagtg
    17401 caaaaagtgc gtgatggtaa ggccgacttt ggcttcaacg cccgtaccga aacctacgag
    17461 aacctgctgg cagcaggagt catcgatccc gttaaagtga cccgtgtggc acttgaaaat
    17521 gcagccagta ttgccggcct gctgctcacc acagaggtga ccatcgttga c
//
LOCUS       DAOMAX010000024        17172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_357_length_17172_cov_5.072326, whole genome shotgun sequence.
ACCESSION   DAOMAX010000024 DAOMAX010000000
VERSION     DAOMAX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17172
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_357_length_17172_cov_5.072326"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..3484
                     /gene="mfd"
                     /locus_tag="PK742_02605"
     CDS             110..3484
                     /gene="mfd"
                     /locus_tag="PK742_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQU75508.1"
                     /translation="MDLQEIINRYRQDDVRTAGIVEQIRKDPRVHLHLKGIPGSLLSF
                     LTTAVYLEKGGHHLLIMNDQEEAGYILDDLRTLLPGKDILFLPDSFRKPGTFDEADTN
                     NVLLRTETINRILHSDTRGEIIVTYPEAIPEKVVRAEVLERSTLLIKKGEKLDGHFIV
                     DVLMENGFVRSDFVFEPGQYSVRGGIIDIFSFGNELPYRVELFDDEVESIRTFEPVSQ
                     LSQKNVAQVSIVPNIQTQFGESVKLPLFDCLPENTCIWFRDASYCGEVFARYHEDAIA
                     WYDLMAQQKHLADHHPFAHHPPEALIASANEWQQGIERFPLLEFGLHSHLQHAISIDS
                     DATPQPVFNKNFNLLLKDLEDHHRQGLDIVIFVSHARQAERFYQIFGDLKATFPYVVL
                     LGSLSGGFIDRQNKLLCYTDHQIFERFHKSQVRSGFARKEAISIKELLQLQPGDYVTH
                     IDHGVGVYAGLEKLDVGGKIQEAVRLKYKDGDLLYVNINSLHKISKFSGKDGTPPKLN
                     KLGSDAWENLKRKTRKQVKDIAKDLIALYAKRRASKGFAFSADSYLQNELEASFIYED
                     TPDQEKATADVKKDMEKSYPMDRLVCGDVGFGKTEIAIRAAFKAVTDGKQVAVLVPTT
                     ILASQHYRTFSERLKDFPCNIDYLNRFKSAKEKKATLEALKDGRTDIIIGTHSIIGKS
                     VQYKELGLLILDEEQKFGVAAKEKLRNLKAQVDTLTLTATPIPRTLQFSLMGARDLSI
                     INTPPPNRQPVTTELKTFDAEFIRDAIYFEIYRNGQVYFIHNRVRDIEEIARLIRQVC
                     PDVSVGVAHGQMEGEELEEHMLRFIDKEYDVLVCTNIVESGLDIPNANTIIINNAHFF
                     GLSDLHQLRGRVGRSNRKAYCYLIAPALYTLPDDSKKRLRTIEQFAELGSGFHIAMRD
                     LDIRGAGNLLGAEQSGFITDIGYDTYHKILDEAIRELKQNEFREVFREEIEEQMDFVQ
                     DVQIDSDLEMLIPDDYINNIAERMAMYTRLDNVRNEQELEAFRQELQDRFGRIPPQVD
                     ELFQAIRLRWVAKDLGFERIIFKLRKLRCYFPENQDAAYYETAFFQRFLQYIASQKAT
                     LHMKQTSRHLLLAMDQVHGMEHARKLLEKIRLDLRN"
     gene            3510..4169
                     /locus_tag="PK742_02610"
     CDS             3510..4169
                     /locus_tag="PK742_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75509.1"
                     /translation="MLQTFLLMMGLLCNPQPQACDIIASTYAEGVTWEFVVNDDKGAM
                     YKTYQYKVDEIYDEEERMVYAIRYVTLNKKGKEEDKGNRNIITAGDLYLVNAETMIPD
                     YKSDKPTNISLPCSPNPGDAVGDVRKVSTYTVENLGQKSYISNKINITDGKYGNRETI
                     TTPLGELDCIVLNYTVSLDLQEFTYKDWIDADLRTVKREIYDKKGKLAFTGMMTSYNK
                     P"
     gene            4181..4870
                     /locus_tag="PK742_02615"
     CDS             4181..4870
                     /locus_tag="PK742_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75510.1"
                     /translation="MRRFILFATILLSAATLFAQMDCDGYFPFREGTSMEYTNYDKKG
                     KLTGRQEQTITEIHDIDGGLEAIFATKLFDDKDKEAMTGDYLLQCKEEGFYVDMSNMI
                     PPDVVESISEMEAEITGEFMEFPRNPKAGQQLPDGNMQIAALVNGTPMMTITLLVTDR
                     LIEGFESVTTPAGTFECMRYSESTEVKMIFRIRARSVSWYAPGVGVVKTESYDDKGKL
                     QGSTVLTRFNK"
     gene            4902..6419
                     /locus_tag="PK742_02620"
     CDS             4902..6419
                     /locus_tag="PK742_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQU75511.1"
                     /translation="MNLLHSVASWYFKREISRIEQVTTRPVETQEAVFRYLMQEARDT
                     EFGKTHDFAGVQQPEDLKKRLPVQDYDTLKPWIERVMNGEQNLLWPSTISWFAKSSGT
                     TSDKSKFIPVSKEALDSCHYQGGRDLLATYLNLHPESKLFSGKGLIMGGSHQVNQLNE
                     HSKYGDVSAVMMQNMPIFGQLVKTPKLSIALMSEWEEKIEKMAEHTVNQNVTHIAGVP
                     TWTIVLIRRLFEMKGTDNLRDIWPNLELYIHGGVSFRPYREQFHQLIRGEGMHYMETY
                     NASEGFFAFQDRADADDMLLMPDYGIYYEFIPAEEFEQEDPQTVGWQDVEAGKNYAVV
                     ISTNCGLWRYKLGDTVRFTTLFPHRIQISGRVKHFINAFGEEVIVDNSDKAIDEACSA
                     THAVMKEYTAGPVYMAGGDGKGGHEWIIEFEQEPDDLSRFTEVLDSTLKSLNSDYEAK
                     RHKDMALVMPLVHIAPTGTFYEWMRSRGKLGGQHKVPRLSNDRKYLEELLVIVHK"
     gene            6425..7105
                     /locus_tag="PK742_02625"
     CDS             6425..7105
                     /locus_tag="PK742_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQU75512.1"
                     /translation="MKIIVTGATGMVGKGVLLECLDDPMVTEVLSVSRRSAGIQHPKL
                     KELLHDNFLDFAPVSAQLAGYDACFHCMGVSSAGMQEADFKKFTYDMSMALAKEVHRH
                     NPQATFTYVSGQGTDSTEQGRIMWARVKGKTENDLLKMGFRQAFMFRPGAIIPRRGIL
                     PSSKLYRFFVRYFTWLIHLIKWLSPKSVVDTDQIGKAMAEVTANGYDKPIITPADILV
                     LAERRDLK"
     gene            complement(7354..10050)
                     /gene="infB"
                     /locus_tag="PK742_02630"
     CDS             complement(7354..10050)
                     /gene="infB"
                     /locus_tag="PK742_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQU75513.1"
                     /translation="MPEEKTIRIAAASREFNVGVQHIVEFLQEKGFEVDAKPNTKLTQ
                     EMYTLLIKEYSKDKVLKEKADQVNIGVARQLDKEEAEKVETVTEVPQVEVPVEVIAEK
                     KTEEEAPKQTEKKEEEPPVQEEQPAEEEVIKARKEKVEMKVLGKIDLESTRKKPAKKT
                     TTKKEEPPVVEEKPVEEPVVKKEEPVVAKKETPPEPEMIETKRVKLEGPKVMGKIIIK
                     EEKKPEKKADGDDKKKRKRIRKPERINVDRHRDTNDRRDNRNKPPLDPEEHQRQIQEQ
                     IRSTLAKLGTGKGKSQKSKIRKHKREVAREEQDGMQPDSSILQVAEFTSLSELASLMN
                     IAPTELVQKCFDLGTMVSINQRLDAEIIELLAEEFGFQVKFINVLEDDEEAEEIADAP
                     ESLLTRPPIVTIMGHVDHGKTSLLDYIRESNVVAGEKGGITQHIGAYEVQTEAGRRIA
                     FLDTPGHEAFTAMRARGAKLTDIAIIVVAADDQVMPQTREAISHAQAAGVPMVFAINK
                     IDKPTANPDKIKEQLSQLNILVEDWGGKFQSQEISAKSGLNVDVLLEKVLLEADLLDL
                     KANPDKEAIGTIIEASLDKGRGYVSNIMVQEGTLKVGDMVVAGPYFGRVKAMFNERNQ
                     KIDKAGPSTPLLLLGLNGAPQAGERFKIYEDEDEAKTVANRRMQIVREQGIRTKKHIT
                     LEEIGRRKALGTFRELNIIIKADVDGSAEALTDSLMKLSTPEVQVNVIFKGVGQITES
                     DIMLASASDAVIVGFQVRPSSAVRTLADKEGVEIRLYSIIYKAIEEVKDAMEGLLAPK
                     VEEKVTCTVEIRETFKISKVGTIAGCYVQDGKITRNTQVRIIRDGIVVHTGTLASLKR
                     YKDDAKEVTAGMECGLNINNFNDIQVGDIIEGFEEVEVKRKL"
     gene            complement(10161..11402)
                     /gene="nusA"
                     /locus_tag="PK742_02635"
     CDS             complement(10161..11402)
                     /gene="nusA"
                     /locus_tag="PK742_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQU75514.1"
                     /translation="MNSLELVESFSEFKDVKNIDRPTLMKVLEDIFRSVIRKKYETDE
                     NFDVIVNTEKGDLEIWHRREIVPDGEVENPATQIALSEALLQEPDFQVGEETYEAVSI
                     ESFGRRAILTIRQTLVQKILELEKDEIFKKYNDRVGDIVTGEVYQVWKKEVLLLDDDG
                     NELILPKGEMIPSDIYKKGDTLRAVVKSVEFRNNSPLVIMSRTSPLFLAKLLEQEVPE
                     VFDGIIVIRKIVRAPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDI
                     INFTNNNSLLIQRALSPARINSIELYNDVMKAAVFLAPDQVSLAIGKGGLNIKLACQL
                     TGYDIDVFRDTDEEEYDVDLDEFADEIEGWIIDELKSIGCDTAKSVLELSVDDLVRRA
                     DLEEETIREVIKILKEEFEED"
     gene            complement(11414..11884)
                     /locus_tag="PK742_02640"
     CDS             complement(11414..11884)
                     /locus_tag="PK742_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75515.1"
                     /translation="MENLTARIEQWLAPKLEELDCFLVDIKVKPSGSKIEVYIDRDKG
                     LQIETCEQVSRFLEFHLDNEADVPSNYNLEVSSPGMDNPFRVERQYRKNIGKTVEVLL
                     HSGVKYEGVLDAYDGEQLTLGIHLPPKKKGQQPEVRSEMFLLKDIKYTKKKISF"
     gene            complement(11974..12171)
                     /locus_tag="PK742_02645"
     CDS             complement(11974..12171)
                     /locus_tag="PK742_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HQU75516.1"
                     /translation="MSAPKKPFALRLGSDIMEALEKWASDEFRSTNGQIEYLLHDALK
                     RAGRLPGKSQDNKDRSSSKSS"
     gene            complement(12168..12698)
                     /locus_tag="PK742_02650"
     CDS             complement(12168..12698)
                     /locus_tag="PK742_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75517.1"
                     /translation="MQLMAYSEYNERSTITDLGLKVLLASSAIMSTGIMLAVYIQADR
                     NGEDTTELLWALALVLVMEVVLFFLIFRSPMIIDGSHEGIQVRYRPFMIRNRVFLWKE
                     VSAWQIRKVSPFGEFGGWGLRRSFRKKKTGLITGGGKALELQLQSGHTWVVSTGNPEM
                     LAMFCRKYAADKEVQV"
     gene            complement(12708..13559)
                     /locus_tag="PK742_02655"
     CDS             complement(12708..13559)
                     /locus_tag="PK742_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQU75518.1"
                     /translation="MMTDKIVKPGSGYLWLILFFLTLAGSIYGMAAVHPAFAIGLLVP
                     VLIISGLFVVNPNDSKVLVLFGAYVGTVKTNGFYFANPFYSKKKVTLRVRNIEGDKIK
                     VNDKEGNPIIIGTVIVWQVTDSAKASFEVDNYEHYVKLQSDAAVRHLAGTYSYDHFDD
                     EQKDITLRAGGDQINAELVKELEERLGLAGVRVLEARITHLAYAEEIAGAMLQRQQAT
                     AVVAARTKIVEGAVGMVEMALQMLSKQQIVELDEEKKAAMVSNLLVVLCSDKAANPVV
                     NTGTLHH"
     gene            complement(13694..14311)
                     /locus_tag="PK742_02660"
     CDS             complement(13694..14311)
                     /locus_tag="PK742_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HQU75519.1"
                     /translation="MRITILGAGNIGMAAAMKWREAGHTITFGVRDVQSQKAYDAVGE
                     GFTVLPFQEAMVDPEVVLFAVPFAIADEVVKAIRSDWENMIIIDATNPVNTPTEPFDS
                     AAEAISAWTGGEVVKAFNTTGVANMRNPNYGDKTIETFICGDDEAAKKVVTQLAEELG
                     FIPVDAGSLEHAAMLENLAKLWITLAYKQGKGPHFAFTLLHRQKS"
     gene            14429..14818
                     /locus_tag="PK742_02665"
     CDS             14429..14818
                     /locus_tag="PK742_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HQU75520.1"
                     /translation="MKKHLLLMALVTTLFVGGAKAQDAMLGEVRLFAGSFAPRGWAFC
                     DGQILNISEYSALFSIIGTYYGGDGRTTFALPDLRGAVPVGVGLSNGANTLDVGESVK
                     LGRDANGVKGLGMHYIICTSGIFPSRN"
     gene            complement(14828..15601)
                     /locus_tag="PK742_02670"
     CDS             complement(14828..15601)
                     /locus_tag="PK742_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQU75521.1"
                     /translation="MQDLRITLIQSDLAWEDPARNLSLFGKLVQSAGETDIIVLPEMF
                     STGFSMNAADLAEAPDGSTVQWMHEMAEKKQCCVVGSVIMEEGDQYFNRLVWMFPDGH
                     YEKYDKRHLFSLAGEENTYTPGHERLLVEVNGWTICPLICYDLRFPVWSRFKNDYDVL
                     LYVANWPEKRVHAWRQLLMARAIENQCYVLGVNRIGVDGNTVYHSGNSAVIDPMGERI
                     WELEHERAVFTTTLKYGYLEEIRTRLPFQNDRDMFSVND"
     gene            complement(15589..16746)
                     /locus_tag="PK742_02675"
     CDS             complement(15589..16746)
                     /locus_tag="PK742_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQU75522.1"
                     /translation="MQSPIIIRSKLPNTGISIFARMTALANQHGAINLAQGFPDFPPP
                     KRLVSLVADAMRKGHNQYAPMPGVPDLREAIADKTKDTYKCKVDAEKEVTVTAGATYG
                     LFAAFQALLHEGDEVLVFEPAYDSYVPGIQLAGAEPVFVELHAPDYSIDWEGVKKKIN
                     ARTRAILINTPHNPSGTCWTKNDLLQLEKIVVGTDIIVISDEVYEHITFDDGRHESVL
                     RYPKLAQQSIVISSFGKTYHNTGWKMGYVIANESITKEFRAVHQFLVFSVNTPMQYAV
                     AQFIREAPEHFQDLPQFYQRKRDFFLEAVKDSRFSFTPASGTYFQLLDYSAISDEQDT
                     VFAERITAEFGVAAIPLSPFYRGPVGDRVLRFCFAKSEKTLEKAAAILCRI"
BASE COUNT         4317 a         4360 c         4087 g         4408 t
ORIGIN      
        1 acaagtttag gaaatattgt gagaacaaag ggttgacaaa aatcttgtac tgaaataaaa
       61 cacaggtcac caaaaacaac cggattccaa gtacctttgc cgtcctttca tggacctgca
      121 ggagatcatt aaccgttacc ggcaggatga tgtgcgcaca gccggtatag tagaacagat
      181 caggaaagac cccagggtac acctgcatct gaagggcatt cccggctcat tactttcctt
      241 tctgacaaca gccgtgtacc tggaaaaggg tgggcatcac ctgctcatca tgaatgatca
      301 ggaagaggcc ggttatatac tggatgacct gcgcaccctg cttccgggaa aagacatcct
      361 gtttctgcct gactcatttc gcaagccggg cacctttgac gaagcagaca ccaacaatgt
      421 gttgttgcgc acggaaacga tcaaccgcat tctgcattca gatacccggg gtgagatcat
      481 cgtaacctat ccggaagcca ttccggaaaa agtagttcgt gccgaagtgc tggaacgaag
      541 taccctgctg atcaaaaaag gtgaaaaact ggatggacat ttcatagtag atgtgctgat
      601 ggagaacggc tttgtacgtt cagattttgt ctttgaaccg gggcagtaca gcgttcgcgg
      661 cggcatcatt gatattttct cctttggaaa tgaattgcca tatcgggtgg agttgttcga
      721 tgatgaagta gaatccatcc gaacctttga accggtcagc caattatcgc agaagaatgt
      781 ggcacaggta agcattgtac cgaacatcca gacccagttt ggagagtcgg tcaagttgcc
      841 cttattcgat tgcctgccgg aaaatacctg catctggttt cgcgatgcat cctattgcgg
      901 agaggtcttc gcccgttacc acgaagatgc cattgcctgg tatgacctga tggcgcaaca
      961 gaaacatctg gcagatcacc atccgtttgc ccaccatccg ccggaagccc tgatcgcttc
     1021 ggccaatgaa tggcagcaag gcatagaacg cttccctctg ctggaatttg gtctgcacag
     1081 tcatctgcag catgccatct ctatagacag cgatgccaca ccccaaccgg tattcaacaa
     1141 gaatttcaac ctgttgctga aagacctgga agaccatcac cggcagggtc tcgatatcgt
     1201 catctttgta tcacatgccc ggcaggcaga acggttctat cagatcttcg gcgacctgaa
     1261 agccaccttt ccgtatgtgg ttctgctggg aagcctgagc ggcggtttta ttgacaggca
     1321 aaacaaactg ctttgctaca cagaccatca gatctttgaa cgtttccata aatcgcaggt
     1381 gcgcagcggc tttgcccgca aagaagccat cagcatcaaa gagctgctgc agctacagcc
     1441 gggagattat gttacacaca tcgatcatgg tgtgggcgtt tatgcaggcc tggaaaaact
     1501 ggatgtgggt ggcaagatac aggaagcggt gaggttaaaa tacaaagacg gagatctgtt
     1561 gtatgtaaac atcaacagcc tgcataagat cagcaagttc agcgggaagg atggcacgcc
     1621 acccaaactc aacaagctgg gaagtgatgc gtgggaaaat ctgaagcgga aaacacgcaa
     1681 gcaggtcaaa gacattgcca aagatctgat agcactgtat gccaagcgcc gggcctcaaa
     1741 aggttttgca ttttctgccg atagttatct ccagaatgaa ctggaagcgt ctttcattta
     1801 tgaagacaca cccgatcagg aaaaggccac tgctgatgta aagaaggata tggaaaaatc
     1861 ttatccgatg gacagactcg tgtgcggcga tgtaggcttc ggcaaaacgg aaatagccat
     1921 acgtgctgca ttcaaggcag tgaccgatgg caaacaggta gcagtgcttg tacctaccac
     1981 tatccttgct tcacaacact accgcacatt ctctgaacga ctgaaagatt ttccctgcaa
     2041 cattgattac ctcaatcgct tcaaatctgc caaagaaaaa aaggccacac tggaggcatt
     2101 gaaagatgga agaactgata tcatcatcgg cacccattcc atcatcggga agtcggtgca
     2161 gtacaaggaa ctgggtctgc tcattctgga tgaagaacag aaattcggag tggctgccaa
     2221 agaaaaactg cgcaacctga aggctcaggt agatacgctg acgctgaccg ccactcccat
     2281 cccccgcacc ctgcagttct cgctgatggg tgcccgcgac ctcagcatca tcaatacacc
     2341 accacctaac agacaaccgg tcaccacaga actcaagacc ttcgatgcag agttcatccg
     2401 cgatgccatc tactttgaga tctatcgcaa cggacaggtg tattttatac acaatcgtgt
     2461 gcgtgatatt gaagagatag ccaggctgat acgtcaggta tgtccggatg ttagtgtagg
     2521 tgtagcacat gggcaaatgg aaggcgaaga actggaagaa cacatgctgc gcttcattga
     2581 taaagaatat gatgtgctgg tatgtaccaa tattgtagaa agcggactgg acattcccaa
     2641 tgccaatacc atcatcatca acaacgccca ttttttcggt ctgagcgacc tgcaccagct
     2701 acgtggcaga gtgggcagat caaacaggaa agcatactgt tatctgatag caccggcctt
     2761 atacaccctg ccggatgatt ccaaaaagcg tttgcgcacc atcgaacaat tcgcagaact
     2821 gggcagcggt tttcatattg ccatgcgtga tctcgatatc cgtggtgccg gcaacctgct
     2881 gggcgcagaa cagagtggct tcattacaga catcgggtat gatacttacc acaagatcct
     2941 ggacgaagcc atccgcgaac tcaagcaaaa tgaattccgt gaggtcttca gggaagaaat
     3001 agaagaacag atggattttg ttcaggatgt acagatcgat tctgatctgg agatgctgat
     3061 acctgatgat tatatcaaca atattgccga acgcatggcc atgtacaccc gtcttgacaa
     3121 tgtgcgcaac gaacaggaac tggaggcatt caggcaggag ctgcaggatc gttttggcag
     3181 gataccaccg caggtagatg aactgtttca ggccatcagg ctgcgttggg tggcaaaaga
     3241 ccttggtttt gaacgcatca tcttcaagct gcgcaaactg cgttgctatt tccccgaaaa
     3301 tcaggatgct gcttactatg aaacagcttt ctttcagcgc ttcctgcaat acattgccag
     3361 ccagaaagct accctgcata tgaaacagac aagcaggcac ctgctcctgg ccatggacca
     3421 ggttcatggc atggaacacg cccgcaaact gctggaaaag atcaggctgg acctccgaaa
     3481 ttgattacct ttaacaacaa acaaccagta tgctgcaaac attcttactt atgatgggat
     3541 tgctttgtaa cccgcaacct caggcttgtg atatcattgc ttctacctat gcagaaggag
     3601 tgacctggga gttcgtggtg aacgatgaca aaggtgccat gtacaagacc tatcagtata
     3661 aagtagatga aatatacgat gaagaagagc gtatggtgta tgccatcagg tatgtgaccc
     3721 ttaataaaaa aggcaaagaa gaagacaagg gaaacagaaa catcatcact gccggcgatc
     3781 tgtatcttgt gaatgcagaa acaatgatcc ctgattataa gagtgacaaa cccactaaca
     3841 taagccttcc gtgcagcccc aatcccggcg atgcagtagg tgatgtgcgc aaggtaagca
     3901 cctataccgt ggaaaacctc gggcagaaaa gctatatatc caacaagatc aatattaccg
     3961 atggcaaata cggtaaccgg gaaaccatca ctacaccact gggagaactt gattgcattg
     4021 tgctgaacta tacggtcagc ctggacctgc aggaatttac ctacaaagac tggatagatg
     4081 ctgatctgcg aactgtaaaa agagagatct acgataagaa aggaaaactt gcctttaccg
     4141 gcatgatgac cagctataac aaaccctgaa aatccgtatc atgagaagat tcattttgtt
     4201 tgctaccata ctattgtctg ccgcaacgct gtttgcacaa atggactgcg acgggtattt
     4261 ccctttcagg gaaggtacca gcatggaata caccaattat gacaaaaaag gaaagctgac
     4321 aggcagacag gaacaaacca ttacagagat acacgatata gacggcggtc tggaagcgat
     4381 cttcgctaca aagctttttg acgataaaga caaggaagcc atgaccggag attacttgct
     4441 gcaatgcaaa gaggaaggtt tttatgtgga tatgtctaat atgatacctc ctgatgtcgt
     4501 cgaatccatc agcgaaatgg aagcagaaat aaccggcgaa ttcatggagt tcccccggaa
     4561 tccgaaagcc ggccagcaat tgcccgatgg taatatgcag atcgctgcac tggtgaacgg
     4621 cactcctatg atgacgatca cattactggt aacagacaga ctgatagaag gatttgaatc
     4681 cgttaccact ccggcaggta cttttgaatg tatgcggtat tctgagtcaa ccgaagttaa
     4741 gatgatcttc aggatacgtg ccaggagtgt aagctggtat gcacccggag taggggttgt
     4801 aaaaaccgaa agctacgatg ataagggtaa gctgcaggga tcaaccgtgc tgacccggtt
     4861 caacaaataa gcacattgcc tcaacctatt gcagccactc tatgaacctg ctacattccg
     4921 ttgccagctg gtatttcaaa cgagagatca gccgcataga acaggtaacc acccggccgg
     4981 tggaaaccca ggaggccgtg ttccgctatc tgatgcagga agcccgtgat actgagttcg
     5041 gcaagaccca tgactttgca ggtgtccagc aaccggaaga cctgaaaaaa cgactgccgg
     5101 tgcaggacta tgacacgctg aaaccgtgga tagaacgggt catgaacggc gaacagaatc
     5161 tgctctggcc atccaccatt tcctggttcg cgaaatcaag tggtaccacc agtgataaga
     5221 gcaaattcat tcctgtaagc aaggaagctc tggacagttg ccattaccag ggtggtcgcg
     5281 atctgctggc cacctacctg aacctgcatc cggaatccaa actgttttcc ggaaaaggac
     5341 tcatcatggg tggcagccac caggtgaatc aactcaatga gcattccaaa tacggcgatg
     5401 tgagtgcggt catgatgcag aacatgccca tcttcggcca actggtaaag accccgaaac
     5461 tctctatcgc cctgatgagc gaatgggaag aaaagatcga gaagatggcc gaacatacgg
     5521 tcaaccagaa cgtcacccat attgccggtg tacccacctg gaccatcgta cttatccgtc
     5581 gcctgttcga gatgaaaggc acagacaacc tgcgtgatat ctggcctaat ctggagttat
     5641 atatacacgg cggcgtgagc ttccgcccct atcgcgaaca gttccaccag ctcattcgcg
     5701 gcgaaggcat gcactacatg gagacctata atgccagcga aggtttcttt gccttccagg
     5761 accgggctga tgctgatgac atgctcctca tgcctgacta tggcatttat tatgagttca
     5821 ttcccgcaga agaatttgag caggaagacc cgcaaaccgt aggatggcag gacgtggaag
     5881 ccggaaaaaa ttatgcagtg gtcatttcaa ccaactgcgg tttatggcga tacaaactgg
     5941 gcgataccgt tcgtttcact acgctcttcc cgcaccgcat ccagatatcc ggacgagtca
     6001 aacatttcat caatgccttt ggcgaagagg tcatagttga caattcagac aaggccatcg
     6061 acgaagcctg cagtgccaca catgctgtca tgaaggaata cactgccgga ccagtttata
     6121 tggcaggcgg cgatggtaag ggcggtcatg aatggatcat cgaattcgaa caggaaccgg
     6181 acgatctgag caggttcact gaagtactgg acagtaccct gaagagcctc aacagcgact
     6241 atgaagccaa gcggcacaaa gacatggcac tggtcatgcc gctggtacat atagcaccca
     6301 caggcacttt ttatgaatgg atgcgcagcc gcggaaaact gggcggacaa cacaaggtgc
     6361 cccgacttag caacgacagg aaatacctgg aagagctact cgtcattgta cataaataag
     6421 aactatgaaa atcattgtaa ctggagctac cggcatggta ggaaaaggtg tattgctgga
     6481 atgtcttgat gacccgatgg tcacagaagt tctttctgta tccagaagat ctgccggcat
     6541 tcaacacccc aaactcaagg agctgctgca cgacaatttc ctggactttg caccggtatc
     6601 agcacagctt gccggttatg atgcctgctt ccactgcatg ggtgtaagtt cagcaggtat
     6661 gcaggaagcc gatttcaaaa agttcactta tgatatgagt atggcgctgg caaaagaagt
     6721 acaccgccac aacccgcagg ccactttcac ctatgtgtcg gggcagggaa cagacagcac
     6781 ggaacagggc cgtatcatgt gggccagggt aaaagggaag accgaaaatg acctgttgaa
     6841 aatgggattc cgccaggctt tcatgttccg gcccggagct atcattcccc gtcgtggcat
     6901 actacccagc agcaaactgt atcgcttttt tgtcagatac ttcacctggc tcatacacct
     6961 gatcaagtgg ctgtcgccga aatcagtggt agatacggat cagatcggta aggccatggc
     7021 agaagtaacg gcgaacggct atgacaaacc gatcatcaca ccggcagaca tactggtgct
     7081 ggcagaaaga cgggacttga aatgagaaaa tgtgagaatg tgagaaatgt gaaaatgtga
     7141 aaatgtgaaa atgtgaaaat gggagaatgg gagaatggga gaatgggaaa atgagaaaat
     7201 tcggtaatta gcgaacagca gacacttata ccaggaacat tcagaaatcc acttactatt
     7261 ccgctcacct cattacgtcc ggcagattct ggcagacagc aatatgacca gcccaacttg
     7321 tgcggcgttt tgcatcagct ggtacgctga ttatcagagc ttccgcttca cttccacttc
     7381 ttcgaatcct tcgatgatat cacctacctg gatgtcgttg aagttgttga tgttcagccc
     7441 gcactccata cctgcagtga cttctttcgc gtcgtctttg tagcgtttga gggatgccag
     7501 ggtaccggta tgcactacga tgccatcgcg gatgatgcgc acctgggtat tccgggtgat
     7561 cttgccatcc tgcacataac aaccggcgat ggtacccacc ttgctgatct tgaaggtctc
     7621 tcggatctcc actgtacagg tcactttttc ttccaccttc ggtgccagga gaccttccat
     7681 ggcatccttc acttcttcga tggctttgta gatgatggag tacaacctga tctctacacc
     7741 ttccttatcg gccagggtac gcactgcact ggagggtctc acctggaagc ccacgatcac
     7801 cgcatctgag gcagaagcca gcatgatatc actttcggtg atctgaccca cacccttgaa
     7861 gatgacgttc acctggactt caggtgtact cagcttcatc aggctgtctg tcagtgcttc
     7921 tgcagaaccg tccacatcgg ctttgatgat gatattcagt tcacggaagg tacccagtgc
     7981 cttgcgtcgg ccgatctctt ccagagtgat atgtttcttg gttcggatac cctgttcacg
     8041 aacgatctgc atccttctgt tggcaacagt cttcgcttcg tcttcatctt catagatctt
     8101 gaaacgttcg cccgcctgtg gtgcgccgtt cagaccgagt aacaataaag gtgtagaagg
     8161 tccggcctta tcgatctttt ggtttctttc attgaacatg gctttgacac gtccgaagta
     8221 tggacctgcc acaaccatat cgcccacttt gagtgtgcct tcctgcacca tgatattgct
     8281 cacgtaacca cggcctttat ccagagatgc ttcaatgatg gtacctatgg cttccttgtc
     8341 tggattggcc ttcaggtcaa gcaggtctgc ttccagcaat actttttcca gcagtacatc
     8401 tacgttgagt ccgctcttgg cagagatctc ctgactctgg aactttccgc cccagtcttc
     8461 taccagaata ttcaattgcg acagttgctc cttgatctta tcaggattgg cagtaggctt
     8521 atctatctta ttgatggcaa agaccatcgg cacaccggca gcctgtgcgt ggctgatggc
     8581 ttccctggtt tgcggcatca cctggtcatc agctgctacc acaatgatgg caatatcggt
     8641 cagtttggca ccccttgccc gcatggcggt gaaggcttcg tgacccggag tatccaggaa
     8701 tgcaatgcgg cgaccggctt ctgtctgtac ttcataagca ccgatgtgct gggtgatacc
     8761 acctttttcg cctgccacca cgttagactc acgaatgtaa tccagcaaag atgttttacc
     8821 gtggtccaca tgacccatga tggtcacgat gggcggtctg gttaacaggc tttcaggggc
     8881 atctgctatt tcttccgctt cttcgtcgtc ttccagtacg ttgatgaact tgacctggaa
     8941 gccgaattct tcagccagca gctctatgat ctcagcatcc agacgctggt tgatggacac
     9001 catggtaccc aagtcgaaac atttctgtac aagttctgta ggagcaatgt tcatcaggct
     9061 ggccagttct gacagcgagg tgaattcagc cacctgcagg atgctggaat caggctgcat
     9121 tccgtcctgt tcttctctgg caacttcccg tttgtgcttt cttattttgc tcttctgcga
     9181 cttgccttta ccggtgccca gtttggccag tgtactgcgg atctgttcct gaatctggcg
     9241 ctggtgctct tccggatcga gtggtggttt gttgcggtta tcgcggcgat cattcgtatc
     9301 gcggtggcga tctacattga tccgctccgg tttccgtatg cgcttgcgtt ttttcttatc
     9361 gtcgccgtct gcctttttct ccggtttctt ttcttccttg atgatgatct tgcccatcac
     9421 cttcggacct tccagtttga cccgtttggt ctcgatcatc tccggttcgg ggggagtctc
     9481 ttttttagca acaacaggct cttctttctt cacaaccggt tcttctaccg gtttctcttc
     9541 cactacagga ggctcttctt tcttggtggt ggtctttttg gctggtttct tcctggtgct
     9601 ttccaggtca atcttaccca gcaccttcat ctctactttc tcttttcggg ccttgatgac
     9661 ctcttcttct gccggctgtt cttcctgaac cggaggttct tcttctttct tttcggtttg
     9721 cttaggtgcc tcttcttccg ttttcttttc agctattact tcaaccggta cttccacctg
     9781 aggcacttca gttaccgttt ccactttttc agcttcctcc ttatccaact gcctggccac
     9841 accaatgttc acctggtctg ctttctcctt gagcaccttg tctttggagt attccttgat
     9901 gagcagggtg tacatctcct gggtgagttt ggtattgggt ttggcatcca cctcgaatcc
     9961 cttctcctga aggaactcga caatgtgctg aacacccacg ttaaactcac gggaggcggc
    10021 ggcaattctt atggtctttt cttccggcat attcactttg ggagatggat tacacgaaac
    10081 catcgcccgg tcattggtta caaagttaag attatccgtt cagcaatcat gtggagcatg
    10141 atgtgccggg cggatcatca tcagtcttct tcaaactcct ctttcaggat cttgatcacc
    10201 tcacggatgg tctcctcttc caggtctgcc cttctcacca ggtcatctac actcagctcc
    10261 agcacgcttt ttgcggtgtc gcagcctatg ctcttaagtt catcaatgat ccatccttca
    10321 atttcatctg caaattcatc cagatccaca tcgtattctt cctcatctgt atcgcggaac
    10381 acatcaatat catacccggt cagctgacaa gccagtttga tgttaagacc gcccttacca
    10441 attgccagcg acacctgatc aggggccaga aaaacggctg ccttcatgac atcattgtac
    10501 aattcaatgc tgttgatcct ggcaggactc agggcacgtt gtatcaggag tgaattgtta
    10561 ttggtaaaat tgatgatatc gatattctcg ttacgcagtt cacgcacaat accatgtatg
    10621 cggcttcctt tcatacctac gcaggcgcct accggatcga tccggtcatc atagctttct
    10681 acagccactt tggcacgttc gccgggtgca cgaacgatct tgcgtatcac gatgatacca
    10741 tcaaacactt ccggcacttc ctgttccagc agcttggcca ggaaaagcgg tgaggtacgg
    10801 ctcatgatga ccagcggact gttatttctg aattctacag acttgaccac agcacgcagg
    10861 gtatctccct ttttataaat atctgaaggg atcatttctc ctttgggcag gatcagttca
    10921 tttccatcat catcaagcag gagaacttct tttttccaaa cctgatacac ctcgccggtc
    10981 acgatgtctc caacacggtc attgtatttc ttaaagatct catctttttc cagttcgagg
    11041 atcttctgaa ccagggtctg acggatggta agaatggcac gtcgtccaaa gctttcaatg
    11101 ctgaccgcct cataggtctc ttcacccacc tgaaagtccg gctcctgcag gagggcttcg
    11161 ctgagggcga tctgggtggc gggattctcc acttcaccat cgggaacgat ctcccgtctg
    11221 tgccagatct ccaggtcacc cttttcggtg ttgacgatca catcaaaatt ctcatccgtt
    11281 tcgtacttct tacgaatgac actgcggaag atgtcttcca gcactttcat cagtgtggga
    11341 cggtctatat tcttgacgtc cttaaattca ctgaagcttt caaccagttc taaactgttc
    11401 atggctgatg gttttagaat gaaattttct tttttgtata tttaatatcc ttcaacaaaa
    11461 acatttctga tcgcacttcg ggttgttgtc cctttttctt cggtggcagg tggataccca
    11521 gtgtaagctg ttctccgtca taagcatcca gcacgccttc gtatttcact ccgctgtgaa
    11581 gcagtacttc caccgtttta ccgatgttct ttctgtactg ccgttctacg cggaaaggat
    11641 tgtccatgcc cggcgatgac acttcgaggt tatagttgga aggcacatcg gcttcattgt
    11701 cgagatggaa ttccagaaaa cgactcacct gttcacaggt ctcgatctgc aggcctttgt
    11761 cgcggtcaat atagacctcg atcttgctgc ccgatggttt cactttgata tccaccagaa
    11821 agcaatccag ctcctccagt ttaggagcca gccattgttc gattcgtgct gtcaggtttt
    11881 ccatgccaca gaaagaaaag aggggacaaa atgccccctc tttcgaagtt gtcacaaaga
    11941 tacggagtct ttggtataaa ccaaattcct gtattagctg cttttagagc tgctcctatc
    12001 tttgttatcc tgtgactttc ccggcagcct gcctgccctt ttcagggcat catggagcag
    12061 gtactcgatc tggccattgg tagaacggaa ttcatcggaa gcccattttt caagagcctc
    12121 cattatatct gagcccaggc gcagggcaaa tggcttctta ggagcactca tacctgtacc
    12181 tccttatctg cggcatattt tctgcagaac atagcaagca tttccggatt accggtactg
    12241 actacccagg tatgcccgct ttgaagttgc aattccagtg ccttgccgcc tccggttatc
    12301 aatccggttt ttttctttct gaatgatctt cgaagacccc agcctccaaa ctcgccaaag
    12361 gggcttactt ttctgatctg ccaggcagac acttccttcc ataaaaacac acggttcctg
    12421 atcataaaag gcctgtaacg aacctgtatg ccctcatggc tgccgtctat gatcattgga
    12481 gagcgaaaaa tcaggaagaa taataccact tccatgacca gcaccagtgc aagagcccat
    12541 agcagctcag tggtatcttc accatttcta tcggcctgta tgtaaacagc cagcatgatt
    12601 cccgttgaca tgatggcaga agatgccagc agtaccttca ggccaaggtc tgtaatggtt
    12661 gatcgttcat tgtattcact gtatgccatc agttgcaata ctacctgtta atgatgtaag
    12721 gtgccggtat tgaccaccgg gttggctgcc ttatcagaac acaagaccac gagcagattg
    12781 ctgaccatgg ctgccttctt ttcttcatcc agttctacta tctgctgttt gctcagcatc
    12841 tggagagcca tttctaccat accaacagca ccctccacga tcttggtacg tgcagcaacc
    12901 actgccgttg cctgctggcg ttgcaacatg gcaccggcta tctcttctgc ataggccagg
    12961 tgggttatac gggcttccag tacccgcaca ccggccagac cgaggcgctc ttccagctct
    13021 ttgacaagtt ctgcattgat ctgatcgccg ccggcacgca gggtgatgtc tttctgttca
    13081 tcgtcaaaat ggtcatagga ataagtgcca gccaggtgac gaaccgcagc atcgctctgc
    13141 agcttcacat aatgctcata attatctact tcaaatgagg ctttggctga atctgtaacc
    13201 tgccaaacga tgaccgtacc tataatgatg gggtttcctt ccttgtcgtt cactttgatc
    13261 ttgtcaccct caatgttgcg aacacgcagg gtgaccttct tttttgaata gaaagggttt
    13321 gcaaaataaa atccattggt ctttacagta cccacatatg ctccaaaaag caccagtacc
    13381 ttactgtcgt tcggattgac cacaaacagt cctgaaatga tgagtaccgg cacaagcagg
    13441 cctatggcaa aggcaggatg tacggccgcc atgccgtata tggagcctgc cagggtcaaa
    13501 aaaaataaaa taagccagag atatcctgat cctggtttaa cgatcttgtc agtcatcatg
    13561 gtaattcaat ttgatatcaa aattatatca ttttggattt acaaacaaga ggcttaaaga
    13621 tttttaacac atagcagcac ctgcaacccc gaaaggtcgt gatctacatt ccaatattat
    13681 ggtatggatg tgctcagctc ttctgtctgt gcagcagggt gaaggcaaaa tgcgggcctt
    13741 tcccttgctt gtaagccagg gtgatccata gtttggccag gttctccagc atggctgcat
    13801 gttccaggct gccggcatca actggaataa aacccagttc ttctgccagt tgggtcacca
    13861 cttttttagc ggcttcatca tcgccacaga taaaggtctc tatggtctta tcaccataat
    13921 tggggttgcg catgttggct accccggtgg tattgaaggc cttcaccact tctccgccgg
    13981 tccaggcgct gattgcttca gctgcagaat caaatggttc tgtgggcgta ttgacaggat
    14041 tggtggcgtc aatgatgatc atattttccc agtcagaacg gatggctttg accacttcat
    14101 cggcaatggc gaacggtact gcgaacaaca ccacttccgg atcaaccatc gcctcctgga
    14161 aaggcaacac tgtgaatcct tcccccacag catcgtaggc tttctggctt tgcacatcac
    14221 gcacaccaaa agtgatggta tgcccggctt cacgccactt catggcagca gccatgccaa
    14281 tgtttcctgc tccgagaatg gtgatgcgca tattgcttgt ttgaatacga atataggcag
    14341 gactctgtaa agcagatgca ccctactacc gatcatttac ctgaaatctg gtttatctta
    14401 tctttatttc cacctataaa acctaatgat gaaaaaacat ctgttactca tggcactggt
    14461 caccacactt ttcgtaggtg gtgcaaaggc ccaggacgcc atgctgggcg aggttcgttt
    14521 atttgcagga agttttgccc cacgcggctg ggcattctgc gatggtcaga tactgaacat
    14581 ctcagaatac tccgctttat tctccatcat cggtacctat tatggcggcg atggacgaac
    14641 cacgtttgcg ctacctgacc tgcgtggtgc ggttccggta ggtgtaggtc tatccaatgg
    14701 agccaacacc cttgatgtgg gtgaatccgt gaagctgggc agagacgcca atggtgtcaa
    14761 gggattggga atgcactata tcatatgcac atccgggatc tttccttcca ggaattagaa
    14821 ccggcactca gtcgttcacg ctgaacatgt cgcggtcgtt ctggaatggc aggcgggtgc
    14881 ggatctcttc cagatacccg tatttcagag tagtggtaaa gaccgctctt tcatgttcca
    14941 gctcccagat acgttcgccc atggggtcga tgaccgcact gttgccactg tgataaacgg
    15001 tgttcccatc cacaccgatg cggttcactc ccagcacata gcactggttc tcgatggcac
    15061 gggccataag taattgtcgc caggcgtgta ctcttttttc cggccagttg gccacgtaga
    15121 gcagcacatc gtaatcgttt ttaaaacgtg accagaccgg aaaacgcagg tcatagcaga
    15181 tgagcggaca aatggtccag ccgttcactt ccaccagcag tcgttcgtga ccgggagtat
    15241 aggtattctc ttcgccggca agtgaaaaca ggtggcgttt gtcatacttt tcataatgcc
    15301 cgtcggggaa catccagacc agtctgttga aatactgatc gccttcctcc atgatgaccg
    15361 agcccaccac acaacattgt ttcttctctg ccatctcgtg catccactgc acggtggatc
    15421 cgtctggtgc ttcggccagg tcagctgcat tcatgctgaa tccggtagag aacatttcgg
    15481 gaaggacgat gatatcggtc tcgccggctg actgtaccag cttgccgaac aaagagaggt
    15541 tgcgggcagg gtcttcccag gccaggtcag attgtatcag tgtaattctc agatcctgca
    15601 taagatagcg gccgcttttt ccagggtctt ttcgcttttg gcaaaacaga aacgcagcac
    15661 cctgtcgcct accgggccgc ggtagaacgg actcaaaggt atggcagcta cgccaaactc
    15721 tgcggtgatc cgttctgcaa agaccgtatc ctgttcatct gagatggcac tgtagtcgag
    15781 cagttgaaaa taggtgcctg atgcgggcgt gaaggagaac cgggaatctt ttacggcttc
    15841 caggaaaaaa tcccgtttgc gctgatagaa ttgtggcaga tcctgaaaat gttccggcgc
    15901 ttcgcggatg aactgcgcca ccgcatactg cataggtgtg ttcacactga agaccaggaa
    15961 ctgatgcact gccctgaact ctttggtgat ggattcattg gcgatcacat aacccatctt
    16021 ccaacctgtg ttgtgatagg ttttgccgaa ggaggatatg acgatgctct gctgtgccag
    16081 cttcggatag cgcagcacgc tttcatgccg gccatcatcg aaggtgatgt gttcatacac
    16141 ttcatcgctg atgacgatga tgtcggttcc caccacgatc ttttcaagct gaaggagatc
    16201 atttttcgtc cagcaggttc cggacggatt gtggggggtg ttgatcagaa tggcccttgt
    16261 tctggcattg atctttttct tgacgccttc ccagtcgatg ctataatccg gtgcatgcag
    16321 ttccacaaaa acaggttctg cgcctgccag ctggataccg ggcacatatg aatcgtaggc
    16381 cggttcgaac accagtacct catcgccttc atgcagcaag gcctggaatg cagcgaacag
    16441 tccgtaagtg gcccctgctg ttacggtcac ttccttttct gcatccactt tgcacttata
    16501 agtgtcttta gtcttgtctg caatggcttc ccgcagatcg ggcacacccg gcatgggtgc
    16561 atactggttg tggccttttc gcatggcatc agccacgagt gaaacgagtc tttttggcgg
    16621 gggaaaatct ggaaacccct gggccaggtt gatggctccg tgctgattgg ccagagcggt
    16681 cattctggcg aaaatgctga tgcctgtgtt gggtaatttg gaacgaatga tgatcggcga
    16741 ttgcatatgg gtaaagtaag gaggaaattg tgaaaggagg aaggacgatg gttagtatcg
    16801 ggtattgagt agggagtatt gggtaatgag acttagtttc ctggcggttg gcatgacttc
    16861 ccgatagtcg aacaacacct tctaaagaac acgttattcc tgaagccgac tcaggattaa
    16921 tgactgcaga ctgataacag gaatctcctg catcacgcca cgtcctgtgg ccagtaacaa
    16981 cgctgaaaca aaataacccc gccataagaa gagctctcat acttgacata gtccaagtgc
    17041 tgtatctaag taatttgtac tttccaaaaa caacttgtaa cttccctaca tgccccattc
    17101 tgccttcatt gttccacatc aaaaaatcag accatgaagg ttaatttcct gttaagcccc
    17161 ccccccccat tt
//
LOCUS       DAOMAX010000025        16770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_371_length_16770_cov_3.945678, whole genome shotgun sequence.
ACCESSION   DAOMAX010000025 DAOMAX010000000
VERSION     DAOMAX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16770
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_371_length_16770_cov_3.945678"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2993
                     /locus_tag="PK742_02680"
     CDS             <1..2993
                     /locus_tag="PK742_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQU75523.1"
                     /translation="GAISACATTYSYGEVEDYSVFIPTPTDYNVGVTDIDMETACGLG
                     MENITVTVTNIGEESVSDFDISYYYDDPVSGLSGTVTEAYAGSPIPSFGSVDYTFTTP
                     ADLSNVGDYTLWAFTSMDLDTVPADDTTFVTFTNIPVISTFPYFEDFEEGTAGWTQYG
                     ISSSWELGSPSAAIISGAPPATPGSENSWATNVDGNYNSYETSYVESPCMDFSSLVLP
                     YMELDIWWDTYAFNDGAQLEYSTDAGATWTLLGDIGTGDNWYTTYGYSLGYDPMIPGY
                     EPGWVGSGGGWKTAHHDIAFLAGESSVKIRIKFASTWYPIYNGIAFDNILIQDPLPDD
                     VAVTAFASPMSAVDLSASEVVAVEITNLGTNTQTSIPVRYQVDGGAVVSETWTGSLAA
                     GETTVYSFSVPYDMSSDGDYNYCAWTALPGDEDISNDTLCDIIKNLAPISGTEAYYIH
                     LSTTDPFSSSDNVDKMDEVFGDGGWIEEYFDTMDPDEIFDEGTCFIYLEGGGSTAIEF
                     ATFLTNNGATVENWVASGGNLFLNAAPEEGGNINVGFGSTSFLYPNYIYYAESTEPDF
                     DIISTPYTPVGTDWTGWWYNFAHAKITGTGLTKLIEDYYTPSTAICSFKEWGDGMVVF
                     GTMTPAEYCSPIDEVGNIRKNIFELLKYCSPVDVGVVDLILPEDGCGLGTEETIEVAI
                     ENFGPTAVSSLTVKYVLDGGPVVTEVATVVVDPGGTEYYTFDATGDFSALGTHTLEVW
                     TDIYVDQDHSNDTLYLELETLEAAVVDLGDDKDVCDSVSLDALNPGMFYYWNTGETTQ
                     VITATEPGDYWVLVTHPVTGCVKSDTVEVDLTYTPDASFDFTMSGYTLDFINTSSPGA
                     DYTWYFGDGGISSEDAPVHTFGVGVYTVVLVAENNCGYSAFDTIIYVGVDAIDAASLE
                     NMVLLYPNPSMGTSVLNIHLDQAEDLRYEIYNATGQLVSSRNLGMVTRINETIHLEDQ
                     PSGIYQVKVFAGEEQVSKDLIIIR"
     gene            complement(3022..3657)
                     /gene="nth"
                     /locus_tag="PK742_02685"
     CDS             complement(3022..3657)
                     /gene="nth"
                     /locus_tag="PK742_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQU75524.1"
                     /translation="MTKKEKAAFIVTRLNELFPDPEVSLRHEDPYTLLIAVLLSAQCT
                     DERVNQVTPGLFRKADNPADMAQVSREEVEDIIRPCGLAPAKSKAIVGLSQILMNQHH
                     GEVPHTFEELEALPGVGHKTASVVMSQAFGVPAFPVDTHIHRLAHRWGLSNGKSVEQT
                     EKDLKRIFDRSLWNKLHLQIIYFGRAYCPARAHDPAACPICSVVGKKKTAP"
     gene            3771..5858
                     /locus_tag="PK742_02690"
     CDS             3771..5858
                     /locus_tag="PK742_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU75525.1"
                     /translation="MSKRYVIFLLLVTLHLSAFTQIQWASRLLSYSSQRGEVSYAAKQ
                     VLGPPSKLPSYGDCGCAWTPRFPDNYEEEFVRVAFDKKIYVSRIYISENHNGGAVKAV
                     MLYDQYNIPHEVYTRTTDKGEYGRLLIIDIPKTTFLTNELKLVLETESVSGYNQIDAI
                     GIADQAIDFQMPAVQNSDYFEYLSNAENLGVSVNSPGSELAPILSDDGSRLYFTRKDH
                     PQNTGSMLNDDIWYAEVKGAKVGPAIHPGEPVNNEINNYVIGVSNGGKMLSLANLYFP
                     DGRSGLGVSQTWAGEGNWRYPVNLITPGLISYNFYAEYYMNTDRSVLLVSLEKGDGMG
                     LKDIYVCFSENQLTWTFPMNLGKQINTAAEEMAPSLSPDGRYLIFSSNGYPGYGEQDI
                     YISKRLDDTWKNWSVPQNLGPEINSIGFDAYFTFNDSSEYAYFTSTRDGYFNPDIYRI
                     QLKPVEEEEEEEEDLLTEQTTSIQPAEPVIETEILPDGVPLVNDVLLFGTIFDAATEK
                     PIDGTVIFSLVNYPSEPDSLETLNHNYRRKVTDQVSYRVVVKKKGYLHYEDTILIAGY
                     NAQRVLREDFRIFPIEKGSSFILDKLNFNANSYVIRRESYEEVDGLLKFMLDNPDISI
                     EIGGHTNGFCDDEFCNLLSKQRAESVMNYLIQHGVEAYRIEAVGYGKSRPIASNDTPE
                     GRTLNQRVEITIR"
     gene            5862..7835
                     /locus_tag="PK742_02695"
     CDS             5862..7835
                     /locus_tag="PK742_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU75526.1"
                     /translation="MRRAILIFVCLYSLTSHGQVVHWADEVVGFSSQKSDLLFSASQA
                     LGKPNKFPDFSFTPCAWMPDLLDRADSTFIHLGFKEPQTVGSVVLVQSFHPEIPVSVY
                     LKQPDGKEIYIPVPANLRSDNIMQIPVLPARPGISTIRIVWNGSMQGDPFQLDALGVM
                     TEPGRVPIPFYTDTLGQINRPEALENLNSPYDEVLPVVSPDGRMLYFDRKKHPLNTGP
                     DLHDDIWYARLMADSSWSAPLNIGPPLNDEGHNFINAVSADGNTLLLGNTYNRTETQS
                     HWMSFSYKTSNGWSTPVPINIIGFQNRDQYNEFSMSADGQYIVLSIESNTTNGMRDIY
                     VSRRISDRYYSAPKNLGPVVNSAATEMSPFLAADGQTLYFASNGWPGYGGYDIYMTRR
                     LDETWEHWTEPLNLGSGINTANWEAYFTTDAMGNMAYFCSSKGGDNLNLYRVPLSPVA
                     QPADLVQFTGKVLNRSSGNMIDATISYVLLSDSVSEEGSTTSLGGNAFNAYLRPDSKY
                     LITINADGYFSEVTEWNSAQADGEQLFYLIPRKVGTAVTLENIYFKVNTSVLVDSSFT
                     ELEKTARFLLDHPDVHIEVRGHTNGLCDDSYCLSLSQRRARSVVDFFIDSGIDEDRLS
                     FAGMGKTEPIADNDTPEGRQRNQRVEFIITKVE"
     gene            7869..9062
                     /locus_tag="PK742_02700"
     CDS             7869..9062
                     /locus_tag="PK742_02700"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQU75527.1"
                     /translation="MTALSHRITALDESATIKMAQLSRDLKAKGHDIIDLSLGEPDFD
                     TPEHIREAAKLAIDTGYSHYTPVAGYPDVRQAISRKFERENGLRYDAAQIVISNGAKQ
                     SIINTVLCLVNPGDEVILPAPYWVSYKAMVQLAEGEPVVLPTTISSDFKITPEQLQAA
                     ITPKTKLMIFSSPCNPTGSVYTRSELEALAAVILQYPDLYVISDEIYEHINFMGKHAS
                     MASVSGMYERTITVNGLSKAYAMTGWRLGYLGAPLDVAKAVDKMQGQYTSAANSITQR
                     AVIAALEGSEEPLRNMQAAFLQRRDLVLSLLKHIDGLKYNIPQGAFYFFPDVTAYFGR
                     SFGHHQITDADALCMYLIEIGGVSVVTGSAFGDPNCIRLSYATSEEVLKEAIRRMEKA
                     LEQLT"
     gene            9059..10060
                     /locus_tag="PK742_02705"
     CDS             9059..10060
                     /locus_tag="PK742_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HQU75528.1"
                     /translation="MKEAHPLQTLFDAFRHLKVLVVGDVMLDNYIHGEVDRISPEAPV
                     PVILVNKRESRPGGAANVAMNIRAMGATPLLVSVIGDDRDGESLMNLLEEEKIDATGL
                     LKDPDRHTTNKTRIIAKGHQMLRLDLEKQADISEELESLVIERAKSLLDEDKPDVLIF
                     QDYNKGLLTERVIRSITMLCLERNIPIAVDPKKKNFFAYQMCTLFKPNLREIRESLLI
                     DVQPGDPESLAMAASALHDRLHNGITMITLADKGVFVRTSRESMTREAHVRNVADVSG
                     AGDTVISVASLCLGAKSDWKLMAALSNLAGGIVCEYPGVVQIDPKRLLKEAKELDLS"
     gene            complement(10061..11317)
                     /locus_tag="PK742_02710"
     CDS             complement(10061..11317)
                     /locus_tag="PK742_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU75529.1"
                     /translation="MYVLWRAIQETFSLVAQELRNNRLRTLLSLLGVTIGILCIISVL
                     SAVDSLRKNIDKGVAKLGSDMLYIQRWPWSFGGEEYPWWEYLKRPVMTYDEYRLLHER
                     LSLAEGVAIESWFNSREVKYTTYSVRNVSVSAVSEDYDKVYALNFASGRYFSPMESNS
                     GAGVALVGYEVGQKLFPPGVDPVGKYIWVLGRKVKVVGYIEKEGESLIDISQDNNILI
                     PYNYVRRVMNMKGPNIDPVIEVKPKEGITAAEIKDEITPLLRNSRKLDPREANDFAIN
                     EVTMAADAFDSLFGVVNMVGFLIGFFSCLVGGFGIANIMFVSVKERTNIIGIKKSLGA
                     KNYLILLEFLIEAIVLSLIGCAFGLLLVFALTQVANRTVNFEFVLSVQNVLFGIMLST
                     ILGVIAGIVPAISASRMDPVDAIRSK"
     gene            11402..12451
                     /gene="queA"
                     /locus_tag="PK742_02715"
     CDS             11402..12451
                     /gene="queA"
                     /locus_tag="PK742_02715"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQU75530.1"
                     /translation="MKLSKFTFDLPENLIAQNPSKERDEAKLMVVHRDSGKIENKKFK
                     NILDYFDEGDVMVLNNTKVFNARLYGRKEKTGARIEVFMLRELNSEMHLWDVLVDPAR
                     KIRVGNKLYFGEDDMLVAEVVDNTTSRGRTIRFIHEGTSEELRRILDLLGETPLPKYI
                     KRKPTDDDKDRYQTVYAKHEGAVAAPTAGLHFTPELLKRLELKGIDFAEVTLHVGLGT
                     FRSIEVEDLSKHKMDAEYVKIDKTAAEIVNKAKLANKRVCAVGTTSMRAVETAVSANK
                     LLKPEEGWTNLFIHPPHEFSICNSMITNFHLPKTSLIIMVAAFAGYDLTMEAYKKAIK
                     DKYRFYSYGDAMLII"
     gene            complement(12517..12738)
                     /locus_tag="PK742_02720"
     CDS             complement(12517..12738)
                     /locus_tag="PK742_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HQU75531.1"
                     /translation="MYWTLELASYLEEAPWPATKDELIDYAIRSGAPIEVIENLQELD
                     DEGEIYEGIEDIWSDYPTQDDFFFDEDEY"
     gene            complement(12867..13418)
                     /locus_tag="PK742_02725"
     CDS             complement(12867..13418)
                     /locus_tag="PK742_02725"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQU75532.1"
                     /translation="MKIYTKKGDDGTTSLIGGTRVPKHHVRVEAYGCVDELNVCIGLL
                     ADQPGTEACMPRLREIQDRLFTIGASLAADPEASRMVLPELRDSDISVLEQDMDQMDA
                     ELPEMKNFVLPGGHPTISFCHLARVTCRRAERQVSFLSEQEEVDQDVIRYLNRLSDYL
                     FVLSRHLAKQLGVAEIAWKPRQA"
     gene            complement(13420..14145)
                     /locus_tag="PK742_02730"
     CDS             complement(13420..14145)
                     /locus_tag="PK742_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU75533.1"
                     /translation="MATAIIELHGIRKAYDLGKMKLEVLKGIDLVIGKNEYVAIMGPS
                     GSGKSTLMNILGCLDKPTSGSYVLNNRKVSSMTENELAEVRNKEIGFVFQTFNLLARL
                     TAVENVALPLIYAARKKQERVRMAEDILQAVGLGHRMNHKPNELSGGERQRVAVARAL
                     VNGPSIILADEPTGNLDTRTSYEIMELFEEIHQQGNTVILVTHEEDIARYAKRIIRIR
                     DGEIERDEVNENKLDPTELKNKL"
     gene            complement(14149..14442)
                     /gene="gatC"
                     /locus_tag="PK742_02735"
     CDS             complement(14149..14442)
                     /gene="gatC"
                     /locus_tag="PK742_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQU75534.1"
                     /translation="MKVDVALIDRLSNLAKLRFEQDEKEDLAGKLESIIGFVDKLQEV
                     PVQGIEPLIHMGDAVNVWRDDAVHMEITKEEALKNAPLADSDYFKVPKVLKKK"
     gene            14573..15574
                     /gene="trpS"
                     /locus_tag="PK742_02740"
     CDS             14573..15574
                     /gene="trpS"
                     /locus_tag="PK742_02740"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQU75535.1"
                     /translation="METVVSGTRPTGVLHLGNYYGAVRNYVRMQEDPNYNGYFFIADF
                     HSLTTHQQPDELGKLVKQTIAAYLASGLDPEKVTIYIQSHLPQIPELYLLMNMLAYKG
                     ELEKVATFKEKIRQHKENVNAGLLTYPVLMATDIIIHRAHKVPVGKDQEQHLEMTRNF
                     AKRFNSRYEVDYFPEPTAFNYGEELVKVPGLDGSTKMSKSGTVNSAIFLDDSDDDIRR
                     KIMRAKTDTGPTAENQERPQEIINLFDLMQIVSTEETYTSFDKAYNNCTIRYGDMKKQ
                     LAEDMIGYIAPIRKKLNELLVDEDHLSKVVLEGREKAWDSASKTLADVKNIMGLNYF"
     gene            15578..16324
                     /locus_tag="PK742_02745"
     CDS             15578..16324
                     /locus_tag="PK742_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQU75536.1"
                     /translation="MAKSSAKKTTTTKAPAKKTAAKKAAPKKAAAPKTALEIPDSSGI
                     RHIAIAGNIGAGKTTLTRALAKHYGWQPHFEDVENNPYLYDFYSDMPRWAFNLQIYFL
                     NSRFRQILDIQQGASTVIQDRTIYEDAQIFAPNLHAMGLMSTRDFQNYTEIFNTLTGL
                     ISPPDLLIYLRASIPALVNQIQKRGREYEDNLRLDYLRRLNEYYEAWISKYKSGKILI
                     IDIETYNFAEKPEDLGEVINMVNRELFGLF"
     gene            complement(16328..>16770)
                     /locus_tag="PK742_02750"
     CDS             complement(16328..>16770)
                     /locus_tag="PK742_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75537.1"
                     /translation="YKLRYRQAGVFEPWNKMNKPLPVFKLTGLECATTYEFQVGSVCL
                     DDGTPVFGGWSELAYETSGDCRIGSVVDELLLYPVPAGTDLHIESPAVISHLFLYAAD
                     GRMISSSAPDVNNCTLDVQMLQPGVYLLHLITAEEVLWREFTVE"
BASE COUNT         4523 a         4188 c         3887 g         4172 t
ORIGIN      
        1 cgggagccat cagtgcctgc gctaccacct actcttatgg tgaagtagaa gattattccg
       61 ttttcattcc cactccaact gactataatg tgggcgttac agacatagac atggaaactg
      121 cctgcggtct gggtatggaa aacataacag ttacagtgac caacatcggt gaagaatccg
      181 ttagcgattt tgatatcagt tattattatg atgacccggt atccggtttg tccggtacgg
      241 taactgaagc ctacgcagga agcccgatac cttccttcgg atcagtagat tatacgttta
      301 ctaccccggc cgacctgagc aatgtgggcg actatacact ctgggcattt acatctatgg
      361 atctggatac ggttccggct gatgatacca ctttcgtaac ctttaccaat attccggtca
      421 tcagtacatt cccctatttc gaagattttg aagaaggcac tgccggatgg actcagtacg
      481 gtatcagcag ttcctgggaa ctgggaagtc cgtctgcagc catcatcagc ggagcccctc
      541 ctgccacccc gggttctgaa aactcctggg ctaccaacgt ggacggcaat tataacagct
      601 atgaaacttc ttatgtagaa agcccttgta tggatttcag cagcctggtt ctcccataca
      661 tggaactgga catctggtgg gatacctacg cattcaatga tggagctcag ctagaatatt
      721 ctacagatgc cggtgccacc tggaccctgc ttggagacat cggtacaggc gataactggt
      781 acactaccta tggatattct cttggatatg accccatgat acccggatat gaacccggct
      841 gggtaggctc tggtggagga tggaaaaccg ctcatcatga cattgccttc ctggcaggtg
      901 aatcatctgt taagatccga ataaagttcg cttctacctg gtacccaatc tataacggga
      961 tcgcttttga taatatcctg atccaggatc cgcttcctga tgatgtagct gttacggctt
     1021 ttgcaagtcc tatgtcggcg gtagatctgt ctgccagtga agtagttgct gtggaaatca
     1081 ccaacctggg taccaacacc caaaccagta ttccagtccg ctatcaggtg gacggtggcg
     1141 ccgttgttag tgaaacctgg acaggatctc tggctgcggg tgaaaccacg gtctattctt
     1201 ttagtgtacc atatgatatg tcatctgatg gcgactataa ctactgtgcc tggacagcac
     1261 ttcccggcga tgaagatatc agtaatgata cgctgtgtga tatcatcaag aacctggcgc
     1321 ctatttcagg cactgaagca tactatattc acctttctac tactgatcct ttcagctcat
     1381 cagacaacgt ggacaaaatg gacgaggtgt tcggagatgg cggatggatc gaagagtatt
     1441 ttgacaccat ggatccggat gagatttttg acgaaggaac ctgcttcatt tacctggaag
     1501 gtggcggatc cactgctatt gaatttgcaa cttttttgac caacaatggc gccactgttg
     1561 agaattgggt agcttctggt ggcaacttat tcctgaatgc cgctcctgaa gaaggtggca
     1621 atattaatgt tggttttggc agcaccagtt ttctgtatcc taactatatc tattatgcag
     1681 aatcaactga gcctgacttt gatatcatct ctacccccta tacaccggtt ggtacagact
     1741 ggaccggctg gtggtacaac tttgcacacg caaagatcac cggaacaggc ctcaccaaac
     1801 ttatagaaga ttactatact ccttccaccg ctatttgctc ctttaaggaa tggggtgatg
     1861 gtatggtggt gtttggcacc atgacaccgg cagaatactg ttctcccata gatgaggtag
     1921 gaaatatccg taagaacata tttgaattgc ttaaatactg ctctcctgtt gatgtgggtg
     1981 tagtggacct cattttaccg gaagacggat gtggcctggg tacagaagaa accatagaag
     2041 ttgctattga aaactttggc ccaaccgcag tttccagcct aactgttaaa tacgttctgg
     2101 atggaggtcc cgtggttact gaagtagcaa ctgtagtagt tgatccgggc ggaacagagt
     2161 attacacctt tgatgccacc ggagattttt ctgctctggg tacccataca ctggaagtat
     2221 ggactgatat ttatgtggac caggatcact ccaatgacac cctgtacctg gaactggaaa
     2281 ccctggaagc tgcagtggtt gatctgggtg atgataagga tgtttgcgat agcgtttctc
     2341 tggatgcgct taatccgggc atgttctact actggaatac cggcgaaacc acccaggtca
     2401 ttaccgcaac tgaacccggc gactattggg tactggttac ccacccggtt accggatgtg
     2461 taaaatcaga taccgtagaa gttgatctga cctacacccc tgatgcctca tttgacttca
     2521 cgatgtctgg ttataccctt gacttcatca atacctcatc gcccggagct gattacacct
     2581 ggtacttcgg cgatggtggc atatcatctg aagatgcccc ggtacataca ttcggagtgg
     2641 gagtttacac cgtcgtgctg gtagccgaaa acaactgcgg atacagcgcc tttgatacca
     2701 tcatttatgt gggtgttgat gccattgatg ctgcaagcct cgagaatatg gtattgctgt
     2761 atcctaaccc aagcatggga acctccgttc tcaacatcca cctggatcag gctgaagatc
     2821 tgcgttatga gatctacaat gccaccggac aactggtcag cagcagaaac cttggcatgg
     2881 tcacaaggat caatgaaacc atccacctgg aagaccagcc ttccggtatc tatcaggtta
     2941 aggtgtttgc aggagaagaa caggtttcca aagacctgat cattatccga tgatcatcaa
     3001 agaatcttat gagacggcac ttcagggtgc cgtctttttt ttacctacta ctgaacagat
     3061 cggacaggcg gccggatcat gtgcacgggc cgggcaatag gctctgccaa agtagatgat
     3121 ctgtaagtgc agtttgttcc acaatgaccg gtcaaaaatc cgcttcaggt ccttttctgt
     3181 ttgttccact gattttccgt tactcaaacc ccagcggtga gccagacggt gaatatgggt
     3241 atcaaccgga aacgcaggta caccgaaagc ctgcgacata accacgctgg cagttttatg
     3301 ccctacgcca ggtaacgctt ccagttcctc aaaggtgtgt ggaacttcgc cgtgatgctg
     3361 gttcatcagg atctgagaca aacccacgat ggcttttgat ttagccgggg ccagtccaca
     3421 gggcctgata atgtcttcta cctcttccct gcttacctga gccatatctg ccgggttgtc
     3481 tgctttcctg aataatcccg gtgttacctg atttacacgc tcatcagtgc actgggcgct
     3541 cagcaatacc gcgatcagca aagtgtaagg atcctcatgc ctgagtgata cttcaggatc
     3601 aggaaacagc tcatttaacc ttgttacaat aaaggccgct ttctcttttt ttgtcatacg
     3661 aatgcctgta aatttaaaag gataaacggg caatgaagtg ttgataagaa ttgacacctg
     3721 taatcataag agcctgaact gaccgttaat tttgcatcat ctgccactgc atgagcaaac
     3781 gttatgtcat ttttctgttg ctggttaccc tccatctgag tgcatttacc cagatccagt
     3841 gggccagccg cctgttgtct tattcatccc agcgtggaga ggtcagctat gctgccaaac
     3901 aagtactggg cccaccttcc aaattaccat cctatggcga ttgcggctgt gcctggaccc
     3961 cgaggtttcc tgacaactat gaagaagaat ttgttcgtgt agcattcgac aaaaagatat
     4021 atgtaagtcg catttacata tcagagaatc acaacggtgg cgccgtaaag gcagtcatgc
     4081 tgtacgatca gtataatatt ccacatgaag tgtacacccg cacaaccgat aaaggagaat
     4141 atggcaggtt gttgatcatc gatatcccga aaactacctt tcttaccaat gaattgaaac
     4201 tggtactgga aaccgaaagt gtttccggct acaaccagat agatgccatt ggcattgctg
     4261 atcaggccat tgattttcag atgcctgctg tacagaacag tgattatttt gaatacctga
     4321 gcaatgctga gaatctgggg gtatctgtaa attctcccgg aagtgagctg gctccaattc
     4381 tcagtgatga tggcagtcgt ttatacttta ccagaaagga ccatccgcaa aataccggct
     4441 ccatgctcaa tgatgatatc tggtatgcag aagtaaaagg tgccaaagta ggaccggcca
     4501 tccatcccgg cgaaccggta aacaacgaga tcaataatta tgtaataggc gtttccaatg
     4561 gcggcaagat gttgtcgctt gccaacctct acttccccga cggacgatcc ggtctgggtg
     4621 tttcacaaac ctgggctgga gaaggaaact ggagataccc cgtcaacctg atcacgcccg
     4681 gcctcatctc ctacaatttc tatgcagaat actatatgaa tacagaccgt tctgtgttgc
     4741 tggtatctct ggaaaaaggc gatggcatgg gactgaaaga catctacgtc tgcttcagtg
     4801 agaaccagct tacgtggacc ttcccgatga acctcggaaa acagatcaat accgctgcag
     4861 aagaaatggc ccccagctta tcgcctgacg gcaggtatct catcttctcc tccaacggat
     4921 accctggtta cggagagcag gacatctata tttccaaacg gctggatgat acctggaaaa
     4981 actggtcagt gccccaaaat ctcgggccag agatcaacag catcgggttt gacgcctact
     5041 tcacctttaa tgacagcagc gagtatgcct atttcacctc tacccgcgat ggctacttta
     5101 accccgacat atacaggatc caactgaaac ctgttgaaga agaagaggaa gaagaagaag
     5161 atttgctgac cgagcaaacc acatccatcc agcctgctga acctgtcatt gaaacggaga
     5221 ttctgccgga tggtgttccg ttggtaaatg atgtcttgtt gttcggtacc atcttcgatg
     5281 ccgctactga aaaacccatt gacggcacgg tcatcttcag cctggtgaat tacccctcag
     5341 aaccggattc tctggaaacc ctcaaccata actacagacg taaagtgacc gatcaggtaa
     5401 gctatcgggt ggtggtgaag aaaaagggat acctccacta tgaagatacc attctgattg
     5461 ccggctataa tgcccagcgt gtgctgcgtg aagacttccg catcttcccg attgaaaaag
     5521 gcagctcctt catcctggac aaactgaatt tcaatgccaa cagctatgtg atcagacgtg
     5581 agtcttatga agaagtggac ggactgctga aattcatgct ggataatcca gatatctcta
     5641 ttgaaattgg tgggcatacc aatggtttct gtgatgacga attctgtaac ctcttgagta
     5701 aacaacgtgc tgaatctgtt atgaactacc tgatccagca tggcgtggaa gcttaccgta
     5761 ttgaagcagt aggatatgga aaatcaagac ctatcgcatc caatgataca ccggaaggtc
     5821 gcaccctcaa ccaaagggtg gagatcacca tccgctaaac tatgcgcaga gcgatcctga
     5881 tctttgtttg tttgtattca ctgacctccc atggccaggt agtgcactgg gcagatgaag
     5941 tggtaggatt ctcttctcaa aaaagtgatc tgctgttttc agcttcacag gcgctgggaa
     6001 aacctaataa atttcctgat ttcagcttca ccccttgcgc ctggatgccc gatctgctcg
     6061 atcgtgctga cagtaccttt attcatttag gattcaaaga accgcaaaca gttggcagtg
     6121 tagtactggt gcaatccttt caccccgaaa ttcctgtttc ggtctatctg aaacaacctg
     6181 acggaaaaga gatctatatt ccggtgccgg ccaatttgcg atcagacaat atcatgcaga
     6241 tacccgttct gcctgcccga cccggaatca gcaccattcg tatagtgtgg aacggaagta
     6301 tgcagggcga tccatttcaa ctggatgccc tgggtgtgat gacagaaccc ggcagagtac
     6361 ccatcccctt ttatacagac acccttggcc agataaacag accagaagct cttgaaaatc
     6421 tgaacagccc ctatgatgaa gtgcttcccg tagttagccc ggacggtaga atgctctatt
     6481 ttgacagaaa aaaacatccg ctcaatacag gacctgacct ccatgacgat atctggtatg
     6541 ctcgtttgat ggccgacagt tcctggtctg ccccgctgaa catcgggccg cctttgaatg
     6601 acgaaggaca taatttcatt aatgccgtca gtgctgatgg caataccttg ctgctgggca
     6661 atacctataa cagaacagag acccagtctc actggatgtc tttcagctat aaaacaagta
     6721 acggatggtc tacaccggta cccatcaata tcattggctt ccagaaccgc gaccaataca
     6781 atgaattcag catgtctgca gatggacagt acatcgtgtt atccatcgaa tcgaacacca
     6841 ccaatggcat gcgagatata tatgtttctc gtcgcatatc tgacaggtac tacagcgctc
     6901 cgaaaaacct tggcccggtg gtaaacagtg ctgcaacaga aatgagtcct ttccttgccg
     6961 ccgacggaca aacactgtat ttcgcctcca acggatggcc cggatacgga ggctacgata
     7021 tctatatgac cagaaggctg gatgaaacct gggagcactg gaccgagccg ctgaacctcg
     7081 gatccggaat caataccgcc aactgggaag catacttcac cacagatgct atgggtaata
     7141 tggcctattt ctgcagctcc aaaggtggag ataaccttaa tctgtatcgt gtaccactta
     7201 gccctgtggc tcagcctgca gacctggtac agttcacagg aaaggtactg aacaggtctt
     7261 caggaaatat gatagatgca acaatcagtt atgtgctgct gtctgattcc gtctcagaag
     7321 aaggttctac cacttcctta ggtggaaatg cattcaacgc ctaccttcgg cccgacagca
     7381 agtatctcat taccatcaat gctgatgggt acttcagtga ggtgactgag tggaacagcg
     7441 cacaggctga tggtgaacaa ctgttttatc tgatccccag aaaagtggga accgctgtaa
     7501 cactggaaaa tatttatttc aaggtgaaca cttccgtgct ggttgattcc agctttacag
     7561 aactggagaa aacagcccgt ttcctcctgg atcacccgga tgttcatata gaagttcgcg
     7621 gacatacaaa cggactttgt gatgactcct actgtctcag cctgtctcaa cgccgggcac
     7681 ggtctgtggt tgatttcttc atcgattccg gtattgatga agaccggctc agttttgcag
     7741 gcatgggaaa aactgaacct attgccgata atgacacccc ggaaggacga caacggaacc
     7801 aacgggtgga gttcatcatt acaaaggttg aatgagcctt cttgtgtaac ttcgcagcaa
     7861 acgatcgtat gacagcattg tcgcatcgta tcactgcact ggatgagtcg gccactataa
     7921 agatggccca gctgagcaga gacctcaaag ccaaaggtca tgacatcatt gacctgagtc
     7981 tcggcgaacc tgactttgat acacctgaac atatccgcga agcagccaaa ctggcaattg
     8041 acacaggtta cagccactac acaccggtag caggctatcc ggatgtgcgt caggccatca
     8101 gcagaaaatt tgaacgcgaa aacggcttac gatatgacgc tgcgcagatt gtcatcagca
     8161 acggcgcaaa acaaagtatc atcaatacgg tactctgcct ggttaacccc ggagatgagg
     8221 tcatcctgcc ggctccatac tgggtcagct acaaggctat ggtgcaactg gctgaaggcg
     8281 aaccagtagt tcttcccact acgatcagca gcgatttcaa gatcacaccg gaacaattgc
     8341 aggcagccat cacaccgaag accaaactca tgatcttcag cagtccctgc aaccccaccg
     8401 gctctgtgta cacccgcagt gaactggaag cactggctgc cgtgatactg cagtatcctg
     8461 atctgtatgt gatcagtgat gagatttatg agcatatcaa ctttatgggt aaacacgcca
     8521 gcatggcatc tgtttccggt atgtatgaac gaaccattac ggtaaacggt ctgtcaaagg
     8581 catatgccat gaccggatgg cgactcgggt atctgggtgc tccgctggat gttgcaaagg
     8641 cggttgacaa gatgcagggg caatacactt ctgctgccaa cagcatcacc cagagagcag
     8701 tgatcgcagc actggaaggc tcagaagaac cgttgcgaaa catgcaggct gcttttttac
     8761 aaagaagaga cctggttctt tcgctattga aacatattga cggactgaag tacaatatac
     8821 cccagggtgc gttctatttt ttccctgacg taactgcata tttcggaaga tcattcggcc
     8881 atcatcagat cactgatgca gatgccttgt gcatgtacct gatcgaaata ggtggtgtat
     8941 ctgttgtgac agggtctgct ttcggcgatc cgaactgcat tcgcctctcc tatgccacct
     9001 ctgaagaagt gctcaaagaa gccatcagac gtatggaaaa agcactggaa caactgacat
     9061 gaaagaagcc catccattac agacattgtt tgatgcattc aggcacctga aagtgctggt
     9121 ggtgggcgat gtgatgctgg ataattacat acacggcgag gtggaccgca tcagtccgga
     9181 agctcctgtt cctgtgatcc tggtcaacaa acgtgaaagc aggccaggtg gcgctgccaa
     9241 tgtagctatg aatatccggg ccatgggagc taccccctta ctggtcagtg tgattggcga
     9301 tgaccgggat ggagaatcac tgatgaatct gctggaagaa gaaaagatcg atgccaccgg
     9361 attgctgaaa gatcctgaca gacataccac caataagaca cgcattattg ccaaagggca
     9421 tcagatgcta cgtctggacc tggaaaagca ggcagatatt tctgaagaac tggaatcgct
     9481 tgtaatagaa agggcgaaat ctttactgga tgaagacaag cctgatgtgc tgatttttca
     9541 ggattacaac aagggtctgc taaccgaacg tgtgatcaga agcatcacca tgctatgcct
     9601 ggagcgcaat attcccatag cggttgaccc caaaaaaaag aactttttcg cctatcagat
     9661 gtgcaccctg ttcaagccaa atttgcgtga gatcagagaa agcctgctta tagatgtgca
     9721 acccggagac cctgaaagcc tggccatggc tgcctcagct ttgcatgaca gactgcataa
     9781 cggaataacc atgatcaccc tggccgataa aggcgtcttt gttcgaacca gtcgcgaaag
     9841 catgacccgg gaagcccatg tgcgcaatgt ggcagatgtt tccggtgccg gtgataccgt
     9901 tatcagcgta gcatcattat gcctgggtgc aaagtctgac tggaaattaa tggcagcact
     9961 gagcaacctg gccggaggca ttgtatgcga atacccgggg gttgttcaaa tagatccgaa
    10021 aagactgctt aaggaagcaa aagagctgga tctttcctga tcacttggag cggatagcat
    10081 caaccggatc cattctggaa gctgaaatag cgggcacgat acctgcaatg acacccagaa
    10141 tggtgctgag catgataccg aacagtacat tctgcacaga cagtacaaat tcaaaattga
    10201 ccgtcctgtt ggccacctgg gtcaatgcga ataccagcag tagcccaaat gcacaaccaa
    10261 taagagaaag cacaatagct tctatcagga actccagcaa aatgagatag ttcttggcac
    10321 ccagagattt cttgatacct ataatattgg ttctttcctt tacggaaacg aacatgatat
    10381 tggcaatgcc aaaacctccc accaggcaac tgaaaaaacc tatcagaaac cctaccatgt
    10441 tcaccacccc gaaaagggaa tcaaaggcat cagcagccat ggtcacctca ttgatggcaa
    10501 aatcatttgc ttctctgggg tccagttttc ggctgttgcg caacaaagga gtgatctcat
    10561 cctttatttc tgcagctgtg attccctcct ttggctttac ttcaatgaca ggatctatat
    10621 tcggtccttt catattcatg acccttcgca catagttata gggtatcagg atattattat
    10681 cctgcgagat gtctatcagg ctttcgcctt ccttttcaat ataccctact acctttacct
    10741 ttctgcccag tacccagatg tacttaccca ccggatccac cccgggtgga aagagcttct
    10801 gacctacttc ataacccaca agtgccaccc ctgctcctga gttcgattcc atcggagaga
    10861 aatacctgcc ggaagcaaaa ttgagagcat acaccttgtc gtagtcttca gaaactgcac
    10921 tcacactgac atttctgaca gaataggtgg tgtacttgac ctcccggctg ttgaaccagg
    10981 attctatggc cacaccttct gccagcgaga ggcgctcatg gagcaggcgg tattcatcgt
    11041 aggtcatgac cggccgcttc aggtattccc accatgggta ctcttcaccg ccaaaagacc
    11101 agggccagcg ctgaatatag agcatatcac ttcccagttt ggctactccc ttgtctatgt
    11161 ttttgcgcag cgaatcaact gccgaaagca cagatatgat gcacaatatg cctattgtta
    11221 ctcccagcag actcaacagg gtgcgcaggc ggttattccg caattcctgg gctaccagac
    11281 taaaggtttc ctgtatggca cgccataaga catacataag ccccaaatat accgagccgg
    11341 gaaacagaaa ttagttttca gattgttaat tttgcaggct tattaaaatc tacactcctg
    11401 tatgaagcta tccaagttca catttgacct tcccgaaaac ctcatagccc agaacccttc
    11461 caaagaaaga gacgaagcca aactgatggt ggtgcaccgt gactccggca agatcgaaaa
    11521 taaaaagttc aagaacatac tggactattt tgacgaaggt gatgtgatgg ttctcaataa
    11581 caccaaggtt ttcaatgcac gtctgtatgg ccgcaaggaa aaaacagggg ctcgtattga
    11641 agtcttcatg ctgcgtgaac tgaacagcga aatgcacctg tgggatgtac tggtagatcc
    11701 ggcccgtaag atacgtgttg gcaacaagtt gtattttggc gaagatgata tgctggtagc
    11761 tgaggtggtg gataacacaa ccagccgcgg aagaaccatc cgcttcattc atgaaggcac
    11821 cagtgaagaa cttcgccgca ttcttgatct gctgggagaa acacctctgc ccaaatacat
    11881 caaacgcaag cctactgacg atgacaaaga ccgttaccag acagtctatg ccaaacatga
    11941 aggagcggtt gctgcaccta cagccggatt acactttact cctgaactac tgaaaagact
    12001 ggagttgaaa ggaattgatt ttgctgaggt gaccctgcac gttggcctgg gcactttccg
    12061 atccattgaa gtggaggatc tgtccaaaca taaaatggat gcagagtatg tgaagataga
    12121 caagactgca gccgagatcg tgaacaaggc caagctggcc aacaagcgtg tttgtgcagt
    12181 tggtacaact tccatgcgtg ctgtagaaac tgctgtaagc gccaacaaac tgctgaagcc
    12241 ggaagaagga tggaccaacc tgttcatcca ccctcctcat gaattcagca tctgtaattc
    12301 catgatcacc aacttccacc tgcccaagac cagtctgatc atcatggtgg cagcgtttgc
    12361 cggatatgac ctgaccatgg aggcttacaa aaaagccatc aaagacaaat accgtttcta
    12421 ctcttatggc gatgccatgc tgatcatctg atcatctgta acataaataa aaaagccctg
    12481 atgttatcag ggcttttttt gttgaccgaa tgttgatcag tattcgtctt catcaaagaa
    12541 gaaatcatcc tgagtgggat agtcagacca gatgtcttct ataccttcat agatctctcc
    12601 ttcgtcgtcc agttcctgca ggttttcgat cacctcaata ggtgcaccgc ttctgatcgc
    12661 atagtcgatc agttcgtctt tggttgcggg ccagggtgct tcttccagat aagaggccag
    12721 ttctagtgtc caatacatat gaaaccgagg gggtttgaat tttgcgcaaa aatatccttt
    12781 ttccgatgct atccaacagt tggcgcaaca aattattgca caattctgga acgggatatg
    12841 aacaggctga aaaaccgggc ctcagatcag gcctgacggg gtttccaggc tatctcagcc
    12901 acacccagct gtttcgcaag atggcggcta agaacgaata ggtagtcgct gaggcggttc
    12961 aggtagcgaa tgacatcctg atcaacctcc tcctgttctg aaagaaagga tacctgtcgc
    13021 tctgcccttc tgcaggtaac acgggccagg tggcagaaag aaatggtagg atgccccccc
    13081 ggtaaaacaa aattcttcat ctccggtagt tcagcatcca tctgatccat gtcctgttca
    13141 agtactgaga tatcgctgtc acgaagttct ggcaaaacca tacggcttgc ctccgggtcg
    13201 gcagcaagac tggcgcctat tgtaaacagt ctgtcctgta tctcccgtaa acgaggcata
    13261 caggcctctg ttccgggctg atctgccagc aaaccgatgc agacattcag ttcatctaca
    13321 cagccatagg cctctacccg cacatgatgc ttaggaacac gtgtaccacc aatcaggctg
    13381 gtagtgccat catcgccctt tttagtatag atcttcaact cagagtttgt ttttcaactc
    13441 ggtaggatcg agtttgtttt cattgacctc atcccgttca atttcaccat cccgaatgcg
    13501 gatgatccgc tttgcataac gtgcaatgtc ttcttcatgg gttaccagga tgaccgtatt
    13561 gccctgctgg tggatctctt caaacagctc catgatctca tagctggtgc gggtatccag
    13621 gttaccggta ggttcatcgg ccagaatgat ggaaggacca ttcaccagtg cacgggctac
    13681 tgccacacgt tgtctttcgc cgcccgacag ttcattgggt ttgtgattca tacggtgccc
    13741 cagtcctact gcctgcagta tatcttctgc catccgtacc cgttcctgtt tttttctggc
    13801 tgcgtagatg aggggaagtg ctacattctc taccgccgta agcctggcca gcagattaaa
    13861 ggtctgaaag acaaacccga tctctttgtt gcgtacttct gccagctcat tctcggtcat
    13921 gctgctcacc tttcggttgt tcagcacata actgcctgaa gtaggcttgt ccaggcaacc
    13981 cagaatgttc atgagggttg acttcccgga accggacgga cccatgatgg ccacatattc
    14041 attcttgccg attaccagat cgatcccctt caacacctcc agcttcattt tgccgaggtc
    14101 atatgccttc ctgataccgt gtagttctat gatagcggtt gccatgtctt acttcttttt
    14161 cagcactttc ggcaccttga aataatcact gtcagccaaa ggtgcattct tcaacgcctc
    14221 ctctttggtg atctccatat gtacagcgtc atcccgccat acgttgacgg catctcccat
    14281 atgaatgagt ggctctattc cctgtaccgg cacttcctgc agtttatcca caaaaccgat
    14341 aatgctttcg agctttccgg ccagatcttc tttctcgtcc tgttcaaagc gaagctttgc
    14401 caggttgctc agcctgtcta tcagggccac atccactttc atgcagtaaa gataagcaac
    14461 ggggtgaagc ctctgcacgc caccatccac atttaacgca tgacaacatt ttttaggtct
    14521 gaccggatct gacaaacagc tgtgccaccg cagttttcct atctttgccg ccatggaaac
    14581 agtggtgagc ggaacacggc caacgggtgt tttgcatctg ggaaattact acggtgcagt
    14641 gcgtaattat gtgcggatgc aggaagaccc gaattacaac gggtacttct ttatcgcaga
    14701 ttttcattcg cttaccaccc accagcaacc ggatgagctg ggaaagctgg tgaaacaaac
    14761 cattgccgca tatctggcca gtggcctgga tcctgaaaag gttacgatct acatccagag
    14821 tcacctgccg caaatacctg aactctatct gctcatgaac atgctggcct ataaaggaga
    14881 gctggaaaag gtggctactt tcaaagagaa gatccgccag cataaggaaa atgtcaatgc
    14941 cggactgctc acctatccgg ttctgatggc tacagacatc atcattcaca gggctcataa
    15001 agtgcctgta ggaaaggatc aggagcaaca cctggaaatg acccgcaact tcgccaagcg
    15061 gttcaacagc cggtatgaag tagattattt cccggagcct acagccttta actacggcga
    15121 agaactggtc aaggtgccag gtctggatgg cagcactaaa atgagtaaat caggtactgt
    15181 aaacagtgcc atattcctgg atgacagtga tgatgacatc agaagaaaga tcatgcgggc
    15241 aaagaccgat accggcccta ccgcagaaaa ccaggaaaga ccgcaggaga tcatcaacct
    15301 ttttgacctc atgcagatcg tgagcacaga agaaacatat acatcttttg ataaggcata
    15361 taacaactgt actatccggt acggcgatat gaagaaacag ctggccgaag atatgatcgg
    15421 atatatcgca ccgatcagaa agaaactcaa cgaattgctg gtggatgaag accatctgag
    15481 taaagtagta ctggaaggcc gtgaaaaagc atgggattct gcttccaaaa ccctggcaga
    15541 tgtaaaaaac atcatgggat taaactattt ttaagccatg gcaaaaagca gcgccaaaaa
    15601 gaccactacc accaaagccc cggcaaagaa aaccgcggcc aaaaaggccg cacccaagaa
    15661 ggcagctgcc cctaagacag cccttgaaat accagattca tctggtatca ggcacatagc
    15721 cattgccggt aacatcgggg ctggaaagac caccctgaca agggctttgg ccaaacatta
    15781 cggctggcag cctcactttg aagacgtaga gaacaaccca tatctctatg acttttattc
    15841 tgatatgcca cgctgggcct ttaacctgca gatctatttc ctaaacagtc gcttccggca
    15901 aatactggat attcagcaag gcgcatctac ggtcatccag gacagaacga tctatgaaga
    15961 tgcacagatc ttcgccccta acctgcacgc catgggactg atgagtaccc gcgactttca
    16021 gaattacacc gagatcttta atacgctgac aggcctgatc tctccccccg acctgctgat
    16081 ctaccttcgt gcttccattc ccgctctggt caaccagatc cagaaacgag gccgtgaata
    16141 tgaagacaac ctcaggctgg attatctgcg cagactgaat gaatactacg aagcgtggat
    16201 atccaaatac aaatctggca agatcctcat catagatatt gagacctaca attttgcaga
    16261 gaagcccgaa gatctgggtg aggtcatcaa catggtgaac cgagagttat tcggtctctt
    16321 ctgatgatca ttccacagta aattctctcc agagcacttc ttctgctgtg atcaggtgta
    16381 acagatacac acccggctga agcatttgca catccagtgt gcagttattg acatcaggag
    16441 cagaactgct tatcatcctt ccgtcagcag catatagaaa taaatgactg ataacagcag
    16501 ggctctctat gtgaagatcg gtgccggcag gcacaggata cagcagaagc tcatcaacca
    16561 cagaacctat gcgacaatct ccgctggtct cataagccag ttctgaccat ccgccaaaga
    16621 caggcgtacc gtcatccaga catacactac caacctgaaa ctcataggta gtggcgcatt
    16681 ccagtccggt cagtttgaat accggcaacg gcttgttcat tttgttccag ggttcaaata
    16741 ctccagcctg cctgtatctc agtttgtatt
//
LOCUS       DAOMAX010000026        16688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_373_length_16688_cov_4.612457, whole genome shotgun sequence.
ACCESSION   DAOMAX010000026 DAOMAX010000000
VERSION     DAOMAX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_16688_cov_4.612457"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK742_02755"
     CDS             complement(<1..516)
                     /locus_tag="PK742_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQU75538.1"
                     /translation="MQKMMQHVFLAATLVVAGSFQTAYGQCTTPGSLAAAPDGSGNMI
                     LSWSDTGADSYTLNLEDAPGNPEVLDITYTGITGTSFTSEILTPGFSYKFKVRSHCGG
                     DHSSWSSFYIFTADGDSVVIGGDDDSTGVGGDDGGIGGGVDDSTGVGGDDSSGVFLCD
                     APDGLMADAAAT"
     gene            777..1697
                     /locus_tag="PK742_02760"
     CDS             777..1697
                     /locus_tag="PK742_02760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU75539.1"
                     /translation="MADMHCKVCGNVEQQRFQQVYAFPTFEVWSCTVCNFHFVPPAFT
                     ELVSYDHYKTKAEQETVRQGNDWLKIQRHKSRLRKINRLQHGGKLFDLGVGWGHFLAA
                     ARDMGYNVSGVELSEYQWEYATRDLGLPVEHMNFFDLPASPGSTDVITMWDVLEHIED
                     AGAVLDRIHDMLCDNGLLVLQVPQIDSYISKKTRDKWSMLSHTHVNYFSPSTITRLLQ
                     DHGFEVLKIRSSIELKLLLMYVIYPRKRKKQGTADSISSDDRQHYFNKVTNRPKWILR
                     IAVFLHDVIYDLLSLLNIGEEMVVYARKKA"
     gene            complement(1701..3083)
                     /locus_tag="PK742_02765"
     CDS             complement(1701..3083)
                     /locus_tag="PK742_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HQU75540.1"
                     /translation="MTKRVQTFLSQSEAKAFDKQHKHKLQFNITKYDQTVVRGKQQYA
                     DLEFVKKRAKNIKYKAIENLDRYLVEFETNFTANGGKVIWANDDKEALQEILKIVKAK
                     KAKTAVKAKSMATEEIHLNEFLAEHGVRSVETDLGEFIQQLDGEPPYHIVTPAMHKSK
                     DDVAKLFADKLGTDINLTAQELTLVAREKLRHEYVQADIGFSGANFILPDIGGIAVTE
                     NEGNARLSTSFPKTHIVVVGIEKMLTGFQDLAMFWPLLATYGTGQKVTVYNTVMTGPR
                     KENEQDGPEEMYVILLDNGRSKLLADEVKREALYCIRCGACLNACPVYKNIGGHTYGT
                     TYSGPIGSVITPHYKGMEEFSHLSFASSLCGNCTEVCPVRINLHNLLLYNRHESVQKG
                     LTSMSEKTAWSLWRRAMLNRKTMERGGAGMKNMAMKVLFRNSWGKHRSMLHFAPKSFH
                     ERWMEMHPEK"
     gene            3303..4565
                     /locus_tag="PK742_02770"
     CDS             3303..4565
                     /locus_tag="PK742_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQU75541.1"
                     /translation="MKQLLRYLPLFFLLMAPAAAFAQGEMAVAGSDGLHLFGIRLEFY
                     LFVATLVGIAFFHHRTLQIALSGLVVILVTKFLTTNFNLVHHLEHEWEILLNLFGLLL
                     GFAILAKHFEESGVPDRIPRLLPRGWAGPLLLLVLVFVMSSFLDNIAAAMIGGSIAIV
                     IFRQNVHIGFIAAIVAASNAGGAGSVVGDTTTTMMWIDGVHPVMLIEAYSASLVAFLI
                     FGFLASRQQFALQPVVFDPNADLRPIRYNKLIICLMILAGAISTNILLDFPAAGVWAA
                     ILIGALFAPTPWGEARHALRGSAFLLSVVLCASMMPVDELPPASWQSAFVLGFVSSVF
                     DNIPLTKLALDQSNYDWGMLAYAVGFGGSITWFGSSAGVAITNLFPEGRHLGKWVRGG
                     WHIIIAYVAGFFAMLLLFGWNPFDHLKG"
     gene            4632..5918
                     /locus_tag="PK742_02775"
     CDS             4632..5918
                     /locus_tag="PK742_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75542.1"
                     /translation="MKKVLAGLFAILLPLLVFASSGSAGSEGLAVAGIRLEFILFALT
                     LMGVALFHNQTLYVALTGLASILILRFLTTDFNLVHHIEHEWEILVNLLGLLLGFAIL
                     AKHFEESGVPDNLPKILPGNWTGAFLLLVLIFVMSAFLDNIAAALIGGSIGLVIFRGN
                     VHIGFLAAMVAASNAGGAGSVVGDTTTTMMWIDGVAIGDVLHAFVAAIPALLIFGYIA
                     AKQQHKHQPIIKDLSAEPKAIDGKRLLVCVMILIGAVTTNVLLDFPAAGVWAAILIGA
                     TFTKTPWKEVPHALPGSVFLLSLVLSASMMPVDQLPAASWQTAFALGFISSVFDNIPL
                     TKLALDQGGYDWGVLAYSVGYGGSMVWFGSSAGVAMSNLYPQAKSVVNWVKNGWHVIL
                     AYVAGFFILYALVGWHPHPPHRDEHKAAAEAEATVH"
     gene            6001..6600
                     /locus_tag="PK742_02780"
     CDS             6001..6600
                     /locus_tag="PK742_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU75543.1"
                     /translation="MNLFSRKEQVTVQDEVLLTQYRISGDLSIVGELYKRYSHLVFGV
                     CMKYLKNEALAEDLSMQVFEKLITDLKNHEVNNFRPWLHMVTRNECLMHLRRDKKMRV
                     VELEPGRTEDDDADVVEFASEEHLDDVHLKEEQLVMLEAGIRELNEDQRTCIELFFLH
                     KRSYQEIADLTGYDLNKVKSYIQNGKRNLRIYMEKRNAG"
     gene            6590..7642
                     /locus_tag="PK742_02785"
     CDS             6590..7642
                     /locus_tag="PK742_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQU75544.1"
                     /translation="MLDKYYHTKDAYYSHDKMVAFLKGELEDYERRAMEKLIDDDPFL
                     RDALEGIKMSGLANTEKVVHRLHKKTDILTGERKPVTISLRARNLAMAAMLLVFLTAG
                     WYIATQLDFNTGSQTAQEYLPEMSAETQTNDPDAMSQQADNTLNEVSQSKQDEGEKYP
                     AIQKELKKQEVTLPEPVESVSNYEVQEDLATGKDNRKETYDFSDTQVTSVPASAEKRS
                     VKMEDDSADEEFMVTDAYLSGTPASESDAIFTTVEVMPQYPGGDKAMQEFIRKELRYP
                     AAAKDASIQGTVYVNFVVDATGEVGNIKLIRGIGGGCDEEAMKMVSKMPRWKPGRQNG
                     KNVPVSMTIPVQFKLQ"
     gene            complement(7634..7897)
                     /locus_tag="PK742_02790"
     CDS             complement(7634..7897)
                     /locus_tag="PK742_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75545.1"
                     /translation="MEQSLPKHDCSKFVEQVFLVLDGEMSEEETNLFIQDIERCSHCL
                     QHYNIEKELKAFLANREERKCCSETLRVSIMQQISDLSDQESL"
     gene            complement(7900..8511)
                     /locus_tag="PK742_02795"
     CDS             complement(7900..8511)
                     /locus_tag="PK742_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU75546.1"
                     /translation="MADHRMSIFTPVSDHRLNKQQKDVIFEREFYPLMDALYNFAFHL
                     VYDEEAANDLVQETFLKAYRFIESYQEGTNAKAWLFKILKNAFINDYRKKSRQPSRVD
                     FDDISGYHDTEESGPTEYVDLRQEVFSGLMGDEVTRALNELPVDFRTVILLCDIEDFS
                     YEEIAKIIDIPIGTVRSRLHRARNILKEKLRNYAANEGYQEKR"
     gene            8555..9790
                     /locus_tag="PK742_02800"
     CDS             8555..9790
                     /locus_tag="PK742_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQU75547.1"
                     /translation="MLSAQKLVSWLPHKLIALAAVAGLMAGLVTTRVVLSVATITLAC
                     NALLHEHVRAHFRLWLKNRNAMLFLGYFLICALSGFYSENLHAWWSSTQNKLPLLAIP
                     FGWTVIHKDLQAYGRALLYAFVMIISVAAAAVLINYFLHYETLNASIKLGSAIPTPMM
                     DHIRFSLETALAALLCLHLLLTGKQFRWLLVLLFLLLGTTLHFLAVRSGLLAFYGGLL
                     LMGLYQLFSGRNRKLGTITLLAGIVIGVMALRFIPSLHNKVLYFNYEWSLIEQGELKA
                     GHSDAQRVQSILYGWEVARQNFPLGVGTGDLRDVMDAEYLQHPDTASIKPERPHNQFV
                     YTLAAIGLAGFVLLLLAFFYPLSDKTSMKAWLMPAYLFIVLISFMTEHVLEIQIGMAF
                     IMLFNHLLPEAFRKKEIIW"
     gene            9787..10545
                     /locus_tag="PK742_02805"
     CDS             9787..10545
                     /locus_tag="PK742_02805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU75548.1"
                     /translation="MKKISAIVTCGNEAAHIQGVLESLSWCDECIVVDSYSEDGTPEI
                     AARFTDKVWQRAYVSPADQKNWAIPQASHEWVLILDADERVTPALEQEIRDLLHANTI
                     PYVAYRIFRINYFMGKRIRFSGWQHDAVVRFFHRDHGRYPDQMVHEELVADGPVGTLK
                     GKLLHFTFKDREHYLQKIDRYARWSALDKESSTGRITWFHTLLKPAFRFVKHFILQGG
                     VLDGYAGWVIATTNARGVALRYRYMLANRNATTS"
     gene            complement(10532..11794)
                     /locus_tag="PK742_02810"
     CDS             complement(10532..11794)
                     /locus_tag="PK742_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQU75549.1"
                     /translation="MRYISILLLIIPASLMAQDQCVDGRYMQDPLFSAADIWISPNVL
                     YASAYDQNGVLRDLMLDVYQPATTVDPLEARPLVIMVHGGGLLGGDKGSQLSVFLCTE
                     LAKRGYVAASVNYRLGWQQLGTCNGDTASLSRARYRAQQDVHAAIRFLEEKSGEYLID
                     TNYIFMAGSSVGATLSLLAAFSSEEDYDPMLVEELGALDSNGNPYADHDRAIAGVFGT
                     GIGMDKPWLLDRNDTPVMCFHGTCDFTVPYVEGPLFYCYEPVQYINFYGSRYLADHMR
                     ETGHPYRIYTNERGGHDAVDSLTLLEQAIPFLRSVLCEEPESKEIYEFHVDGCIVNRM
                     DSVELDIHPNPVQEVLSVTIGSGSTGTAVIEIFNSIGQQVLYYEEAFYAPVREMEIIL
                     PPAMEERGIYFLRVRIQESVGMETFMKW"
     gene            complement(11796..13394)
                     /gene="nadB"
                     /locus_tag="PK742_02815"
     CDS             complement(11796..13394)
                     /gene="nadB"
                     /locus_tag="PK742_02815"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQU75550.1"
                     /translation="MRNTDFLVIGSGIAGLTCALKMARRFPHMQVTVITKAEKDETNT
                     KYAQGGIAAVWDFETDSYQSHIEDTLTAGDGLCNRKIVEIVVKEGPERVKEIIHWGAD
                     FDKEADGDYALGREGGHSVNRVLHHKDITGKEIEKTLLEEVHELENVTLLQHHFVLDL
                     ITQHHQGRIVTRHTPDITCYGAYLLNKENGDIEKILARITLMASGGAGQVYKSTTNPV
                     IATGDGIAMVYRAKGRIANMEFVQFHPTALYDPGVSPSFLITEAIRGSGAVLKTASGE
                     PFMQKYHKLESLAPRDIVARAIDQEMKQSGDDFVYLDATGIAEEEIMHHFPNIFETCL
                     SKGIDIRKDMIPVVPAAHYMCGGIMTDEHGATSIRNLYACGECTCTGLHGANRLASNS
                     LLEAMVFAHRIYLQAATHLDQEELQADIPDWNADGTTDPREMVLITQSMRELKEIMTN
                     YVGIVRSNERLDRALKRLHLLYEETERLYDTATLSPQLCELRNLITIGFLIARGAVLR
                     KESRGLHYTTDYPEKGSWAEDTLL"
     gene            complement(13461..14036)
                     /locus_tag="PK742_02820"
     CDS             complement(13461..14036)
                     /locus_tag="PK742_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HQU75551.1"
                     /translation="MIIIEEKLVSEDILDVEFACNLTACKGACCVEGDEGAILEEQET
                     DILKKIYPVVQEYLTEEGRKAIEKQGYHIVNNEGELKTPLINNGPCAYVTYQDGVALC
                     GIEKAYRAGKLNWPKPVSCHLYPIRINKVGEYDALNYEKWDICKPACKHGKKLHVPVY
                     RFVKDALIRKYGPSFYEALDATFVYRQEQEE"
     gene            complement(14039..14743)
                     /locus_tag="PK742_02825"
     CDS             complement(14039..14743)
                     /locus_tag="PK742_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQU75552.1"
                     /translation="MSKLFLIPTELDAAAPDMIPAEVHRITSSLDLFFTEKERTARRY
                     LRKTGYDRSFDEVAIHRLDKDTTQEEMELFIRELSSRNGGLLSEAGLPCVADPGAGLV
                     ALAHQNGIRVVPLSGPSSLMLALMASGFNGQQFAFNGYLPVQSAERKEVLRKLEHRIY
                     SEGQTQLFIETPYRNGSLFQDIMQCCRPDTWLCVASGLTGEGESVRSMPVSSWKKKHM
                     NWEKVPAVFVLGRPYL"
     gene            14772..15365
                     /locus_tag="PK742_02830"
     CDS             14772..15365
                     /locus_tag="PK742_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HQU75553.1"
                     /translation="MKLTKQFYRRTDTTAVAEELLGCLLHVRTPEGICTGRIVETEAY
                     CGREDRGCHAFGGRRTPRTEVMYLPGGVTYIYICYGIHQMFNIVTHVKDEPHAVLIRA
                     VEPVSGIALMQQRRQTKELHRLARGPGLVGQAFQLDTRMNGLSLTGNDIWLTREPGFE
                     TGTVIKSPRVGMNFEGFYKTVPWRFRISGSQFTSPAR"
     gene            complement(15537..16346)
                     /locus_tag="PK742_02835"
                     /pseudo
     CDS             complement(15537..16346)
                     /locus_tag="PK742_02835"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
BASE COUNT         4031 a         4234 c         4014 g         4409 t
ORIGIN      
        1 ggtagcagcc gcatcagcca tcagtccgtc aggtgcatca cacaggaaaa cgccggatga
       61 atcatcgcca ccaactccgg tgctgtcgtc cacaccacca cctattccgc catcatcgcc
      121 accaactccg gtgctgtcgt catcgccacc tatgacaact gaatcgccat cggcagtaaa
      181 aatgtaaaag gaactccagg aagaatgatc accaccgcaa tgactgcgca ctttaaactt
      241 gtagctaaag cctggtgtca ggatctctga agtgaacgat gtaccggtaa tacctgtgta
      301 agtaatatcc agcacttccg ggttacccgg agcatcttcc agattcaggg tataggaatc
      361 tgcgccggta tctgaccagc tgaggatcat gttgcctgat ccgtcaggtg cagcagccag
      421 tgaaccgggt gtagtacatt gcccgtaggc tgtctgaaag gatccagcca ctaccagggt
      481 tgcggccaga aatacatgtt gcatcatttt ttgcatactg attggtttaa tgaatgaata
      541 atcccggtgt tgttgcttct gatgcaaaag tgaggcggaa tggaaaggga ggaaagaaca
      601 gatctaatgt atttccgctg gatgttggca ttctgcatcc cgatcatcct gtcatggttt
      661 ggtctaaaca cccgtcctgc aagggtttcg tgcaaaaatt tcgtgggaca tcatttcgat
      721 acattcagaa atcacatgcc tttttgacgt gcaggaagct cttatctttg cacgtgatgg
      781 ctgatatgca ttgcaaggtt tgtggaaatg tagagcaaca acgctttcag caagtatatg
      841 ctttccccac atttgaagtg tggtcctgca cagtatgtaa ttttcatttc gttccgcctg
      901 cattcacgga acttgtcagc tatgatcact ataagaccaa agctgagcag gaaacggtca
      961 ggcagggaaa tgactggctg aagatccagc gacacaagtc tcgtttaaga aagatcaatc
     1021 gacttcaaca cggtggtaaa ctctttgacc ttggcgttgg gtggggacat tttctggcgg
     1081 ctgcacgaga catgggttat aatgttagcg gtgtggaatt atcagaatat cagtgggaat
     1141 atgctacccg tgatctggga ctgcctgtag aacacatgaa tttctttgac ctgcctgcat
     1201 cgcccggaag caccgatgtc attaccatgt gggatgtgct ggagcacatt gaagatgccg
     1261 gtgctgtttt ggatcgcatc catgacatgc tgtgtgataa cggattgctc gttctgcagg
     1321 tacctcagat agacagttac atcagtaaaa agacaagaga taagtggagt atgctcagcc
     1381 atacccatgt aaattatttt tcaccctcca ctattaccag gctgttgcaa gatcacggat
     1441 ttgaggtgct gaagatcagg tcttccatag aactgaagtt gttgttgatg tatgttatct
     1501 acccgaggaa gcgcaaaaaa cagggcactg ctgattcgat cagttctgat gaccggcagc
     1561 actatttcaa taaagtgacc aatcgaccca agtggatatt gcgcatcgct gtattcctgc
     1621 acgatgtcat ctatgacctg ctatctctgc tgaatatcgg agaggagatg gtggtctatg
     1681 cgaggaagaa agcttaacac ctatttttca ggatgcatct ccatccacct ttcatggaaa
     1741 gacttaggtg caaaatgcag catgcttctg tgtttgcccc agctgtttcg gaacaacact
     1801 ttcatcgcca tgttcttcat acctgcaccg ccgcgttcca tcgtctttct gttgagcatg
     1861 gctctgcgcc acaaagacca ggctgtcttc tctgacatgg aagtaaggcc tttctgaaca
     1921 gattcatgac gattatacaa aagcaggtta tgcagattga tgcgcaccgg acatacctct
     1981 gtgcaattgc cacaaagcga tgaagcgaaa ctcaggtgag agaattcttc catgcccttg
     2041 tagtgtgggg taatgaccga accgatcgga ccgctgtagg tagtaccata cgtatgacca
     2101 ccaatgttct tatacaccgg acaggcattc agacaggcgc cgcagcgaat gcaatacagg
     2161 gcctctctct tcacttcatc ggccagtaac ttactgcgac cattgtccag cagtatcaca
     2221 tacatttctt ccggaccgtc ctgttcattc tcttttcgcg gaccggtcat aaccgtgttg
     2281 taaacggtta ctttctgacc cgtaccatag gtggccagca agggccagaa catggccagg
     2341 tcctgaaatc ctgtcagcat cttttcaatg cccaccacca cgatatgtgt ttttgggaaa
     2401 ctggtactca gtcgggcatt gccttcattc tctgtaacag caattccacc gatgtccggt
     2461 aaaatgaaat tggctccgct gaatccgata tctgcctgca catattcatg tcgaagtttt
     2521 tctcttgcca ccagggtaag ctcctgtgcc gtcagattga tatcggtacc cagtttatcg
     2581 gcaaagagtt tagctacgtc gtccttgctc ttgtgcatgg ccggagtgac gatgtgatag
     2641 ggtggttctc catccagctg ctggatgaac tctcccaggt cggtctctac actgcgcaca
     2701 ccatgttcgg ccaggaattc gttgaggtgg atctcttctg tggccatgct cttggccttg
     2761 accgccgtct tcgccttttt ggccttgacg atcttcagga tctcctgtaa agcctccttg
     2821 tcatcattgg cccagatgac cttcccgcca ttggctgtaa agttggtttc gaactctacg
     2881 aggtagcggt ccaggttctc aatggccttg tatttgatgt tcttggcacg tttcttcacg
     2941 aattccaggt cagcatactg ctgcttaccc ctgaccacgg tctggtcata cttggtgata
     3001 ttgaactgaa gcttgtgctt atgttgctta tcaaaagcct ttgcctcact ttgggacaga
     3061 aaagtctgta cacgtttggt cataccgtgt gtgctggttc tgacaaaaat acgctaccgg
     3121 cagcataccg tttcccacac cctctgcggg tcaaaaaaat agttctccat acatgtttgg
     3181 aatctccaac ttcgccatac atttgatgca cttttccaac aggcacctat ggctatcctg
     3241 cttcagccgc accctgcttc catgcgcatt gcatggcttg cttaccatta agtatcggtt
     3301 tcatgaaaca attactcaga tacctgccac tcttttttct gctgatggca ccggcagccg
     3361 cattcgcaca gggagagatg gctgttgccg gatcagatgg tttacatctc tttggcattc
     3421 gcctggaatt ctatctcttc gtggccaccc tggtgggcat cgcattcttc caccatcgta
     3481 cactgcagat cgctttgagc ggactggtag tcatcctcgt gacgaagttt cttactacca
     3541 acttcaacct ggtacaccac ctggaacatg aatgggagat cctgctgaac ctgttcggat
     3601 tgctgctggg ctttgccatt ctggccaaac attttgaaga gtctggggta cctgacagaa
     3661 ttccgcgttt gctgccccgt ggctgggccg gacctcttct tcttttagta ttggtgtttg
     3721 tcatgtcatc cttcctggac aatattgccg ctgccatgat cggtggctcc attgccatcg
     3781 tcatcttccg gcagaacgtg cacattggtt tcatcgcagc catcgtagcc gcatccaatg
     3841 ccggtggtgc aggctctgtt gtgggagaca ccaccactac catgatgtgg atagacggtg
     3901 tgcatcctgt gatgttgatc gaagcctatt ctgccagcct ggtagccttt cttattttcg
     3961 gttttctggc ttcccgtcaa caatttgcat tgcaaccggt ggtcttcgat ccgaatgcag
     4021 acctccgtcc tatccggtac aacaaactca tcatttgcct gatgatcctg gcaggtgcta
     4081 tttccaccaa catcctgctg gactttccgg ctgcgggcgt ctgggcagcc atcctgatcg
     4141 gtgccttgtt tgcacccaca ccctggggcg aagcccggca tgcattacgc ggttctgcct
     4201 ttttgctgtc ggtggtattg tgtgcttcca tgatgcctgt ggatgaattg cccccggctt
     4261 cctggcagtc agcgtttgtg ctgggttttg tatcttctgt tttcgataac attccactta
     4321 ccaagctggc gctcgatcag agcaactacg actggggcat gctggcctat gcggtaggct
     4381 tcgggggttc catcacctgg tttgggtcat cggccggagt ggccatcacg aacctcttcc
     4441 ccgaagggcg ccatctggga aaatgggtga gaggtggctg gcatatcatc attgcctatg
     4501 tggcaggatt cttcgccatg ctactactat tcggctggaa tccgttcgat cacctcaaag
     4561 gttgaatcat cgcccgcatc ttcggtcaag ttggtgtatc ttagcgcccg ctaaactgaa
     4621 ccacattacg catgaaaaaa gtgctggcgg gacttttcgc cattctcctc ccactcctgg
     4681 tatttgcttc atccggttct gcaggcagtg aaggcctggc agttgccggc atacgcctgg
     4741 aattcatatt atttgcgctt accctcatgg gtgtagcctt gttccacaac cagaccctgt
     4801 atgttgcgtt aaccggactg gcatccatcc tgatactgcg gttcctgacc acagacttca
     4861 acctggtgca ccatatagaa catgagtggg agatcctggt caacctgctt gggctgttgc
     4921 ttggttttgc tattctggcc aaacactttg aagagtcggg cgtacctgat aacctgccga
     4981 agatccttcc cggcaactgg accggagcat ttctgttgct ggtacttatt ttcgtgatgt
     5041 ctgcctttct ggacaatatc gcagcggcac tcattggtgg ttccatcggc ctggtcattt
     5101 tcagaggtaa tgtacacatc gggtttctgg cggccatggt agctgcatcg aatgccggtg
     5161 gtgccggttc tgtcgtgggc gataccacca ctaccatgat gtggatagac ggagtggcca
     5221 ttggagatgt gcttcatgca ttcgttgcag ccattccagc cctgctcatt ttcggatata
     5281 tagctgccaa acaacagcat aaacaccagc ctattatcaa ggatctctca gcagagccca
     5341 aagccattga cggaaaaagg ttactggttt gtgtcatgat cctgatcggt gcagtcacta
     5401 ccaacgtatt actggacttt ccggcagcag gtgtctgggc agctatcctg atcggagcaa
     5461 cgttcacaaa aacaccctgg aaggaagttc ctcacgcact tccgggttca gtattcctgc
     5521 tcagtctggt actcagtgca tctatgatgc ctgttgatca gctgcctgca gcctcctggc
     5581 aaaccgcctt tgcactggga ttcatttctt ctgtgttcga taacataccg cttaccaagc
     5641 tcgcactcga tcagggtggc tatgactggg gggtgctggc ctattctgta ggctatggag
     5701 gctctatggt atggttcgga agctctgcag gggtggccat gtccaacctg taccctcagg
     5761 ccaaatcagt ggtcaactgg gtgaagaacg gatggcatgt gatcctggct tatgtggcag
     5821 ggttctttat cctgtatgcg ctggtgggat ggcatccgca ccctccgcac agggatgaac
     5881 acaaggctgc tgctgaagct gaagccactg ttcactaagt cacaatttaa cgcagaattt
     5941 acccagctgc catccattct gcgtagattt actgaccgtt agcaaaacaa gcggcaggtt
     6001 ttgaatctat tcagccggaa agaacaagtc acggttcagg atgaggtgct gctgacccaa
     6061 tacaggatca gtggtgatct gtctattgtg ggagagctct acaagcgata ctcccacctg
     6121 gttttcggtg tttgcatgaa atatctgaag aatgaggcat tggccgaaga tctgtctatg
     6181 caggtctttg aaaagctgat cacagacctc aagaaccacg aggtcaacaa tttcaggccc
     6241 tggctgcaca tggtaacacg caatgaatgt ctgatgcacc tgcgccgcga taaaaaaatg
     6301 cgtgttgtgg agctggaacc gggcagaact gaagatgatg atgcagatgt tgtggaattt
     6361 gccagcgaag agcatctgga tgatgtacac ctgaaagaag agcaactggt gatgctggaa
     6421 gccggcatcc gtgagctgaa tgaagaccaa cgaacttgta tagagttgtt cttcctgcat
     6481 aaacggagtt accaggaaat agctgatctg acagggtatg acctgaataa ggtgaaaagt
     6541 tacattcaga acggcaagag aaatctgcgc atttatatgg aaaaacgaaa tgctggataa
     6601 gtactatcat accaaagacg cttactacag ccatgataaa atggtggcct tcctaaaggg
     6661 agaactggaa gactatgaac gaagggccat ggaaaaactc attgatgatg acccattcct
     6721 gcgggatgcc ctggagggga taaaaatgag tggcctggcc aacacagaga aggtggtaca
     6781 ccggttgcat aaaaagaccg atatcctgac cggcgaacgc aaaccggtaa ccatatcact
     6841 ccgtgcccgc aatctggcca tggctgccat gctcctggta tttctgaccg cgggatggta
     6901 tatcgctacc caacttgatt tcaacacagg ttctcagact gcacaggagt acctgccgga
     6961 aatgtctgcc gaaacccaga caaatgatcc ggatgctatg agtcaacagg ctgacaacac
     7021 cctgaatgag gtctcacagt caaaacagga tgaaggtgaa aagtatccgg ccattcagaa
     7081 agaattgaaa aagcaggaag tgaccctgcc tgaacccgtg gaatcagtaa gcaactatga
     7141 agtacaggaa gatcttgcca ccggaaagga taacagaaaa gagacctatg atttctctga
     7201 tacacaggta accagtgtgc cggcttctgc agaaaaaagg tcagtgaaaa tggaagatga
     7261 ctcagctgat gaagagttca tggtcactga tgcctatctg agtggcaccc cggcatctga
     7321 aagtgatgcc atctttacta cggtagaagt gatgcctcag tatcccggtg gcgacaaggc
     7381 catgcaggaa ttcataagga aagaactgag gtaccctgct gctgccaaag atgcttccat
     7441 tcagggaacc gtgtatgtga actttgtagt ggatgccacc ggagaagtag gaaacatcaa
     7501 actgataaga ggtattggcg gaggttgtga tgaagaagca atgaagatgg tcagcaaaat
     7561 gccaaggtgg aagcccggtc gtcagaatgg taagaatgtg cctgtatcca tgaccattcc
     7621 tgtgcagttc aaattacaat gactcctgat cgctcagatc actgatctgc tgcatgatgc
     7681 tgacccgcaa ggtttctgag caacattttc tttcctcccg gttggccaga aaagctttca
     7741 gctccttctc aatattgtag tgttgcaggc aatgtgaaca acgttctatg tcctgaatga
     7801 agagattggt ctcttcttct gacatctctc cgtccagcac caggaatacc tgctccacaa
     7861 acttgctgca atcgtgttta ggtaaagact gttccatgct cagcgctttt cttgatagcc
     7921 ctcgttggct gcatagtttc taagtttctc tttcaggata ttcctggccc ggtgcaatct
     7981 ggagcgcact gttccaatag gtatatcaat gatcttcgcg atctcttcat aagagaagtc
     8041 ttcaatatcg cataacagga tgaccgttct gaaatcaacc ggcagttcat tcaaagccct
     8101 ggtcacttca tcacccatca gcccgctgaa cacctcctga cgcagatcta catattcagt
     8161 aggtccgctt tcttccgtat catgataacc ggaaatgtca tcaaaatcta cccgcgaggg
     8221 ctggcgggat ttcttccggt aatcattgat gaaggcattc ttaagtatct tgaacagcca
     8281 ggcttttgca ttggtgcctt cctggtatga ttctataaag cgatacgcct ttaaaaaagt
     8341 ttcctgtaca aggtcattgg cagcttcttc atcatatacc agatgaaaag caaagttgta
     8401 gagggcatcc atgagcggat aaaactcccg ttcaaaaatg acatcttttt gctgcttgtt
     8461 taaccgatga tcactcacgg gggtaaaaat agacattctg tggtcagcca tcatacaaca
     8521 ggcagatgga gggaaatttt tacatttgga gcacatgctc agcgcacaga aactggtttc
     8581 ctggctgccc cacaaactca ttgctctggc cgcggtggca ggtctgatgg ctggactggt
     8641 cactacaaga gtagtactga gtgtggccac catcaccctg gcctgtaatg cactgctgca
     8701 tgaacatgtg cgtgctcatt ttcgcctttg gctgaagaac cggaatgcta tgctctttct
     8761 gggttacttc ctgatatgtg ccctgagtgg tttttattca gaaaacctgc atgcctggtg
     8821 gtccagtacc cagaataaac tgccactgct ggctattccg ttcggatgga ccgtcattca
     8881 caaagacctg caggcatacg gccgtgcctt gctgtatgct tttgtcatga tcatatctgt
     8941 tgccgctgct gcagttttga tcaattactt tttgcactac gaaacgctca atgcctctat
     9001 caaactgggc agtgccattc ccacacccat gatggaccat atacgattca gcctggaaac
     9061 agcactggct gcacttttgt gcctgcatct cctgctaacc ggaaagcagt ttcggtggct
     9121 gctggtcctg ctctttctgc tgcttggaac cactttacat ttcctggcgg taagaagcgg
     9181 gctgcttgca ttctacggcg gacttctcct catgggtctc tatcagttgt tttcaggcag
     9241 gaacagaaaa cttggaacca tcacgctcct tgcaggtatt gtcattggag taatggcact
     9301 tcgtttcata ccatcgctgc ataacaaagt actgtacttt aattacgaat ggtctcttat
     9361 tgaacaaggc gaactcaagg ccggacacag cgatgcccag cgggtacaaa gcatacttta
     9421 cggttgggag gtggccaggc agaactttcc gcttggagta ggtaccggcg atctgcgtga
     9481 tgtgatggat gctgaatacc tgcagcaccc tgacacagca tcgatcaaac ctgaaagacc
     9541 acacaaccag ttcgtatata cactggcagc tattggtctt gccggttttg tcctgttgtt
     9601 gctggctttc ttttatccgc tttctgataa gacatccatg aaggcctggc tgatgcctgc
     9661 ctatctgttc atcgtgctga tcagttttat gaccgagcat gtgctggaga tccagatcgg
     9721 gatggcgttc atcatgctct tcaaccacct tctgccggaa gcatttcgaa aaaaggagat
     9781 aatctggtga agaagatctc agcaattgtt acctgcggaa atgaagctgc tcacattcaa
     9841 ggtgtgctgg aatcgctttc ctggtgcgat gaatgtatcg tggtggacag ctattctgaa
     9901 gatggtacac ctgaaatagc tgccaggttc acagacaagg tctggcaacg ggcctatgtc
     9961 agtccggctg atcagaagaa ctgggctatt ccacaggctt cacatgaatg ggtgctcatt
    10021 ctggatgccg atgaaagggt aaccccggcc ttagagcagg agatcagaga cctgttacat
    10081 gccaatacca ttccatatgt agcttaccgg atcttccgta tcaattactt catgggaaaa
    10141 cgcatccgct tcagcggatg gcaacatgat gccgtagtgc gtttctttca ccgggaccac
    10201 ggcaggtatc ctgaccagat ggtgcatgaa gaactggttg ccgatggccc tgtcggtacc
    10261 ctgaaaggaa aactactgca cttcactttt aaagaccggg aacattacct gcagaaaatt
    10321 gaccgctacg cccgatggtc tgcactggac aaggaatcaa gtacaggccg cattacctgg
    10381 tttcacacct tactgaaacc tgccttccgg tttgtgaaac atttcattct ccagggtggt
    10441 gtactggatg gctatgccgg ttgggtcatt gctactacaa atgcccgggg tgtggcactt
    10501 cgttacaggt atatgctggc taacagaaac gctaccactt cataaaggtc tccataccca
    10561 ctgactcctg aatgcgtacc cgcaaaaaat aaatgccacg ctcctccata gcgggtggca
    10621 ggatgatttc catttctctg accggtgcat aaaaggcctc ttcgtagtag agcacctgct
    10681 gaccaatgga attgaatatc tctataacag cagttccggt ggacccgctt ccaatggtga
    10741 cagacagcac ttcctgtacc ggattcggat ggatgtccag ttcaacagaa tccattctgt
    10801 tcactataca tccatctaca tgaaattcat atatttcttt tgactcaggt tcttcacaca
    10861 gaacagaacg cagaaatggt atggcctgct ccagcagggt cagtgagtca accgcatcat
    10921 gcccgcctct ttcattcgtg tatatcctgt acgggtggcc ggtttcccgc atatgatcag
    10981 ccagatacct ggaaccgtaa aagttgatgt attgtaccgg ctcataacaa tagaacagcg
    11041 gaccctctac atatggaacc gtaaaatcac aggtgccatg aaaacacatg accggtgtat
    11101 cattcctgtc cagcagccag ggcttatcca ttccaattcc ggtaccgaat acgccggcta
    11161 tggctctgtc atgatctgca tatggattac cattgctgtc cagagctccc aactcttcta
    11221 ccagcatcgg gtcatagtct tcttccgatg aaaatgctgc cagcagtgac aaggtggctc
    11281 ctacgctgct gccggccata aaaatatagt tggtatcaat gaggtactca cctgacttct
    11341 cctccagaaa gcgaatggct gcatgcacgt cctgttgtgc ccggtacctg gccctggaca
    11401 aactggctgt gtcgccattg caggtaccca attgctgcca gccaagacgg tagttcacgg
    11461 aagcagccac atatcctctt tttgccagct ctgtacagag aaatacagaa agctgactcc
    11521 ccttgtcgcc accaagcaag ccacctccat gtaccatgat caccagtggt ctggcttcca
    11581 gcggatcgac cgttgtggcc ggctggtaca catccagcat cagatcacgc aatacaccgt
    11641 tctgatcata tgcactggca tacagtacat tcggactgat ccagatatca gctgcactga
    11701 acaatggatc ctgcatatac ctgccatcca cacactgatc ctgagccatc agagaagccg
    11761 gtatgatcag caataaaata gaaatgtatc gcatactata aaagcgtatc ttctgcccag
    11821 cttccttttt ccggataatc tgttgtgtaa tgcagccccc ggctttcctt tcgcagtaca
    11881 gctccacgtg ctatcagaaa accgatggtg atcaggttgc gcagttcaca caactgtggc
    11941 gacagtgtag ccgtatcata taatctttct gtttcttcat acaacaggtg aagtcttttc
    12001 aaagccctgt ccagacgttc attgcttcgc acgataccca catagttggt catgatctcc
    12061 ttgagctcac gcatgctttg ggtgatcaat accatctctc tcggatcagt ggtgccatcg
    12121 gcattccagt caggaatatc tgcctgtaat tcttcctggt ccagatgggt ggctgcctgc
    12181 aggtagatgc gatgggcaaa caccatggct tccagcaaag agttgctggc caacctgttg
    12241 gcaccatgca aaccggtgca ggtacattct ccgcaggcat acagattcct gatgctggtg
    12301 gcaccgtgtt catcggtcat gatgccccca cacatataat gtgcggcagg aaccacaggg
    12361 atcatgtctt ttctgatatc tatgcctttg gacaaacagg tctcaaaaat gttggggaag
    12421 tggtgcatga tctcctcctc ggcaataccg gtagcatcca gatagacgaa atcatcgccc
    12481 gactgtttca tctcctggtc gatggcacgg gctacgatat cacgtggtgc cagcgattcc
    12541 agtttgtgat atttctgcat gaaaggttca ccggaggccg tttttaacac agctcctgaa
    12601 cctcggatgg cttctgtgat aaggaaggat ggagacactc cggggtcata caatgcagtg
    12661 ggatgaaact ggacgaattc catgttcgct atgcgtccct tggcccggta caccatggcg
    12721 ataccatcgc ccgtagcaat gaccggattg gtggtgctct tatagacctg tcctgcaccg
    12781 ccactggcca tcagggtgat acgagccagg atcttttcaa tgtcgccgtt ctccttgttg
    12841 agaagatagg cgccatagca ggtgatgtct ggcgtatgcc tggtcacgat tcgaccctgg
    12901 tggtgctggg taatgaggtc cagcacaaaa tgatgctgca gcagggtcac attctccagc
    12961 tcatgtactt cttccagcag ggtcttctcg atctctttgc cggtgatgtc cttatggtgc
    13021 agtacacggt tcacactgtg tccgccttcc ctgcccagtg cataatctcc gtctgcttct
    13081 ttatcaaaat ctgcccccca gtgaatgatc tcttttaccc gttcggggcc ttcctttact
    13141 acgatctcca cgatcttcct gttgcaaagt ccatcgccgg cggtcagggt atcttcaata
    13201 tgcgactggt aactgtcggt ctcaaaatcc caaacagccg ctattccacc ctgtgcatat
    13261 ttggtattgg tctcatcttt ttcagccttg gtgatcaccg tgacctgcat gtgcggaaac
    13321 ctgcgggcca ttttcagagc gcaggtgagt ccggcaatgc cactgcctat taccagaaaa
    13381 tctgtatttc gcatacgggt taaaattaga ccattattca aaacgaaggc tgtatgcggc
    13441 aaacacggct ttgtcagata tcattcttcc tgttcctgtc tgtacacaaa agttgcgtcc
    13501 agggcttcat aaaaagacgg gccatacttt ctgatcaggg cgtctttcac aaaccgatac
    13561 acaggtacat gtagtttttt gccatgtttg caggcgggtt tacagatgtc ccacttttca
    13621 taattgagtg catcatattc acctaccttg tttattctga tgggatacaa gtggcaactg
    13681 acaggcttgg gccagttgag ttttcccgca cggtaggctt tttctattcc gcataacgcc
    13741 acaccatcct gataggtcac ataggcacag ggaccattgt ttatgagtgg tgttttaagc
    13801 tccccttcat tgtttacaat gtggtatccc tgtttttcaa tggcttttct gccctcttcc
    13861 gtgagatatt cttgtacaac gggatagatc ttctttagta tatctgtttc ctgctcttcc
    13921 agtatggccc cttcatctcc ttccacgcag caggctccct tacaggcggt caggttacag
    13981 gcaaattcga catcaagaat gtcttcgctg accagtttct cttcaatgat gatcatgttc
    14041 aaaggtaggg tcttcccaag acaaaaacag cgggcacttt ctcccaattc atatgcttct
    14101 tcttccagga agaaaccggc atgctgcgca ctgactctcc ttctccggta agtccggaag
    14161 ctacacacag ccaggtatct ggccggcagc attgcatgat gtcctgaaaa agtgatccgt
    14221 tgcggtaggg tgtttcaatg aacagctggg tctgtccttc tgaatagatc cggtgttcca
    14281 gtttacgcaa cacctctttc cgttctgccg actgtaccgg cagatacccg ttgaacgcaa
    14341 actgctgacc gttgaatccg gaagccatca gcgccagcat aagcgaagaa ggaccactca
    14401 atggtaccac cctgattcca ttttgatgtg ccaacgccac cagtcctgca cctggatctg
    14461 ccacgcaggg taagcctgct tcactaagca atccgccgtt gcgggaagac aattcccgga
    14521 tgaacaactc catttcttcc tgtgtggtat ccttgtccaa ccggtgtatg gccacctcat
    14581 caaaagagcg gtcgtatcct gtcttgcgca ggtaacggcg ggctgttctt tctttttcgg
    14641 tgaagaagag atcaagagaa gacgtgatgc gatgcacctc tgcaggaatc atatcaggtg
    14701 cggctgcatc cagttctgtg ggaatgagaa aaagcttcga caccttccgg aaatttttca
    14761 atttaggtgc gttgaaatta accaaacaat tctacaggcg aacagacacg acagcagttg
    14821 cagaagagtt gctgggatgt ttgcttcatg tgcgtacacc tgaaggcatc tgcaccggac
    14881 gcatcgtgga gaccgaagca tattgcggtc gggaagatcg gggatgtcac gcattcggtg
    14941 gccgacgtac cccccgaacg gaagtgatgt atctgccggg gggagtgacc tatatctaca
    15001 tctgctacgg cattcaccag atgttcaaca tcgtgaccca tgtgaaagat gaaccacatg
    15061 ctgtgctcat cagggctgtg gaacctgttt caggtattgc actcatgcag cagcgaaggc
    15121 aaacgaaaga gctgcaccgg cttgcccgcg gaccgggtct tgtaggtcag gcatttcaac
    15181 tggataccag gatgaatgga ctttcactga ccggaaatga tatctggctg acccgggaac
    15241 ccggctttga aactggtact gtaataaaga gccctcgtgt aggaatgaac ttcgaaggat
    15301 tctataagac ggtgccctgg cgattccgta tctccggttc gcaatttacg agtcctgccc
    15361 gctgaggcct gtaacacagg taacaacggt atctgttgcc tgagctgcac ctgaccatct
    15421 tcatccagca cctgcaaggt gatcgatcct gacagatcat ccggcagatg cacctcacca
    15481 aaattcttac ctaaaaatat ccttgaagac tctatgtcat tcctgtacct tgtcggtggt
    15541 cgttcggaat gggttactcc gctggacacc agttcggtgt acaacctgcc gtgatactct
    15601 tgcgtgttga tctcgccgaa atgtttatca cctgacacaa agacaaccgg tacatcaatc
    15661 agagccagcc tgtcaaacaa ccttttcctg gatgccggat aattctgcca tccttcaaaa
    15721 caatgatcat ctgacagaac ggaagtgcct gatacgatca gccacacatc agcccgaata
    15781 gacaacaaag aatcaaacca gtgccactgt tcttcaccaa gcatatctgc ttcagctgaa
    15841 ggctgctcgc ggaaataacg ggtatccagc agtatcagac ctgctgttat ttcaccatga
    15901 tgtatccatt caacggtaaa ggccgcgtcg cggtggcttc tttcagaact gctgtcctct
    15961 cccagaaatc tgcgaagcag agcagcactt tcttttttcc agggataggt cgcatctccg
    16021 ttgttctgac cgaaatcatg gtcatcgtat atgccggtta ccggtacctc cgcacatagt
    16081 ctttgatagc cctctgactg ctggagcagc gcatacttgt gtgcgatctt gtccaggttg
    16141 tttccgggcc acggtaccgg ccagtcagga tagatgatgt cgccggtcca taccaccaga
    16201 tccggatccc tttcccgcac acgatcaaat atgcctgttt gtttttgctg gttattgcag
    16261 gaaagaaaag tgatggtacc agctgcatca ggcagcggta tatgcgcatg aggggcaggc
    16321 tgcaggtaga aaaaactgca acccatcatg ttggttccaa gagacaggag gtagaacacc
    16381 gctacgtaca gacgtagccg gcggaagata acaggcagtt cacggacaac tgttggcatc
    16441 cctttgatac ttgaacagcc caaaagtatc cgtgcagtat tacctgggtt tcagagcaat
    16501 actgtgattt caataagaaa caggaagcct ttgttagcac aatcctgcaa agacgttaag
    16561 tgaatatcaa ttctgttttt tttgtatgca gtagttgaca cccggcctgt ttcccaccgg
    16621 gtgatgtgga agtcattcca gccaatgttt cagaagggac ggcgtttatg agcataaatt
    16681 attctgcc
//
LOCUS       DAOMAX010000027        16624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_374_length_16624_cov_3.835778, whole genome shotgun sequence.
ACCESSION   DAOMAX010000027 DAOMAX010000000
VERSION     DAOMAX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16624
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_16624_cov_3.835778"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK742_02840"
     CDS             <1..969
                     /locus_tag="PK742_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQU75554.1"
                     /translation="RETPGQARRDSSTGVLSVVSYLSLLNTHYLRPMTPIIQTIMLHR
                     KQVDVLRLDKMTTGGIGNKYYKLRYNLLAAKEQGLKSILSFGGAFSNHLHALSAIGKQ
                     EGFQTIGVVRGEDDPDNPTLQDLRNNDMHLHFISRQDYRQKNDASFIRSLEERYGSFY
                     LLPEGGSNSLAVQGCREILQGVKQDYDHILVAVGTGATVAGIVATPELTSRVTGIAVL
                     KGEDGLSEVVQKFIEPYEINTTWDISFRYHLGGYARYNADLMEYIRNIRDENELETDP
                     VYTSKVVWACADMLEKNEIRQDEKVLLIHTGGMQGWTGWNYRFRIS"
     gene            1379..2746
                     /locus_tag="PK742_02845"
     CDS             1379..2746
                     /locus_tag="PK742_02845"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQU75555.1"
                     /translation="MDFISKYIETNKERMLDELLEVLRIPSVSADPKYKADVEKTAHW
                     MAGKLKAAGADNVEVCPTEGHPIVYGDKLIDPSKPTVLVYGHYDVQPADPLELWNSPP
                     FEPVVKDGKIYARGSCDDKGQVYMHVKAFEAMMANNALPVNVKFIIEGEEEVGSAHLE
                     TFIKENTERLSCDVILISDTSIVANDVPSITTGLRGLCYMEIEVTGPNRDLHSGVYGG
                     GVANPLNTICDMISSLIDEKGHITIDGFYDDVLVVSAEERAEMAKTPFDLEEYKKDLG
                     IGEVKGEDGYTTLERVSIRPTLDVNGIWGGYTGEGSKTVLPSKASAKISMRLVPNQSP
                     DKIAKLFEAHIKKIAPPYVSVKVIAHHGGEPVVVPLDFAPMQAAMKAMEQSFGITPIP
                     TREGGSIPIVALFEQVLGAKSILMGFGLNSDAIHSPNEHYGLFNYYKGIETIPWFFKY
                     YAEMN"
     gene            2881..4716
                     /locus_tag="PK742_02850"
     CDS             2881..4716
                     /locus_tag="PK742_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQU75556.1"
                     /translation="MSAKPVLLILSLIITGGIRSMAQPIIDLELITTGFNEPVDIASA
                     GDERLFIVSRHGTIEILYPDLSVSTFLDIDDRVGSGGGEQGLQGLVFHPDYASNGFFY
                     VNYTDTLGDTHISRFSVSAGDPDLADPDSELLLIYADQPANNHNSGDLNFGPDGYLWL
                     CTGNGGGPANGNAQDTTSILGKVLRIDVDGGSPYAIPADNPFIGEPGVDEMWALGLRN
                     PWRFSFDRLTGDLWIADVGGAIKEEVNFRPVSSGGGENYGWQCYEASYQSALCDTSID
                     FTFPIFEYNHNPFTGGYAITGGFVYRGNAYPHLYGYYTTIDYVTGNAWALHSDGMGGW
                     LVDTLGFLVDHITSFGEDQNGEIYACEKDAGNIYRLKDLCGSFALTSDITDETALGVS
                     DGSIDLTVTGGNAPYSISWSTGASSEDLSGLSADTYTVTVTDDLGCVQTGVYEVAQLC
                     GPAIDVQVSDITTSSATISWSPSGAGLYKVQYITMGGTPGQITTPDTSVTLSGLDAGA
                     VYVFRVRNKCSGTTATYTASGAFMTDPLRDGMVSGLEVFPNPTTGLLYLQSDMQDTPV
                     MIRDVSGNPVMQYSVCPEVIDLSGLPNGIYILEIHGRSQQIILQH"
     gene            5066..6988
                     /locus_tag="PK742_02855"
     CDS             5066..6988
                     /locus_tag="PK742_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQU75557.1"
                     /translation="MKKIKLLGLIIIAYATSAFAQMGVINEDLKILSDDAATGDSFGQ
                     SVVLDGGMILIGAPFDDDNGLNSGAAYIVDAATGAQLFKLLPDDGVAGAEFGYAVAMK
                     DGVAAIGARADTELGAGAGAAYLFDAATGTQLYKLVPDDLSAGDEFGNAIDIDGGVVA
                     VGAWRADEFGEASGAAYLFDVASGTQLEKLLPDEGNNFQTFGVSVAIDAGVVAVGART
                     FFVLGDGYTFAKAFLFDVSSGDLLNTVQPDILNYNGDLGGHFADCLDMKDGLLVVGTP
                     NRSVFFDFSGAIYIFNVGTGEQLHFIYPEDGHDRDHFGISVSLDNDVVTIGAFEDDDS
                     GFDGGSAYYFDAISGDQISKLLASDGEAFDRFGVSVSTQDGLTVIGAKQDDDGGDNAG
                     AAYLFGDISACTGFSLMANITDETADGLSNGSIDLTITGGTEPFMISWSTGDFTEDLS
                     ALEAGSYSVTVTDDLGCMATGIYEVAQLCGAVTGLTVSDISTNTATISWNSSGASIYK
                     VQYITTGSSPGVVNTTDTTVTLTGLLPGSNYVFRVRNKCTGTTTTYTASGAFMTDPLR
                     DGMVSGLEVFPNPTTGLLYLQSDMQDTPVLIRDVSGNPVMHYNVCPDVIDLSGLPNGM
                     YILESGSERMIVLVQH"
     gene            complement(6995..7783)
                     /locus_tag="PK742_02860"
     CDS             complement(6995..7783)
                     /locus_tag="PK742_02860"
                     /inference="COORDINATES: protein motif:HMM:NF039649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6090 family protein"
                     /protein_id="HQU75558.1"
                     /translation="MAKFLRTFRQNSMAESRIGRYLLYAVGEIILLVIGILIAVQINL
                     RNEAAKNERAESIYIQNILKDIDAQQKSIEVQVDYESSYISSAKYLIETLENKEIQVD
                     QKFAAELANLLSRRTFVVQDATYTDLISSGKINLINDEGVRNDLISYYQEMERMEKVI
                     YHNNSDIIDGNISHVLEEFGYINYSFEKLNHQGYLKNSNSAYHLGHFDEQFADISTQI
                     MTDPRVRLHVMNAVKKRLLIATIHHEMMDGATTLSNELKNRFYE"
     gene            complement(7815..8492)
                     /locus_tag="PK742_02865"
     CDS             complement(7815..8492)
                     /locus_tag="PK742_02865"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HQU75559.1"
                     /translation="MTTHSQLKKTARLTGIWYLLMAISGLLGFMVFHPQLFVDDPQQT
                     LTNLQEQESLARVRLVLEFIIIISQALTAVWFYRLFKGINEWGAWTLGIWGMVNSVAI
                     MISAIAMGTALEVAWRTTDATDGVLASITTLSSLVTNAWGIGSLFFGLWLIPMGYIVT
                     SSTCMLLWLGRTLIIGGAGYLLSTFLQYLGMDGTATELLVIPATIGEFWMIGYLLIFG
                     IRQTSNA"
     gene            complement(8516..9553)
                     /locus_tag="PK742_02870"
     CDS             complement(8516..9553)
                     /locus_tag="PK742_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQU75560.1"
                     /translation="MRINGHGHLLPEPSQIPSFMKEKKLFWIDEDKRFMRQGDWSRPV
                     THPSFFLEEKLEWMRLNQIDHEVLLCLSQLYCNGWEETDCHDAIRFQNDFNAQVQADH
                     PDKFTCGFVVQPLYIDQALREIERCVKDLHLKLLCLPTHFLNKQGEWLSTAEADVDPI
                     YEMANEYNLAIQIHPYDGEKMIALKDAYWRFHLVWMMAQCADTLHIYTLRGLPDTFPH
                     IRTSFAHGGMLGIASYGRRIQGFDGRPDLFRGLQDPRKTLGHQNIYFDTLVHDSYTLE
                     LLKKRVGSSQIMMGLDDPYPLGEMEGIGTSYPGRVVDYAVKKGILTEQEGKDIWHKNV
                     LDWLGIEWKNH"
     gene            10026..11426
                     /locus_tag="PK742_02875"
     CDS             10026..11426
                     /locus_tag="PK742_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75561.1"
                     /translation="MVFSDNILENRIKSDHTWWETKQIPFYTELNKRRYFTPFYGLIQ
                     QSALHRAVVLMGPRRIGKTVMMYQAIQDLLDSGIEPKKILYFSLDTPIFTNVSLDELI
                     FRALTPNDESIEDCFIFFDEIQYLKNWEIHLKSLVDRNRRTKFIASGSAAAALRMKSI
                     ESGAGRFSDFFLPPLTFAEYIDLNGYNASLVKIEVDFGGPRRFYKTEDIEQFNRHFTD
                     YINFGGFPEIALNKEEVKNPEQVIQKDITDKVIMKDLPGLFGIENTLELQQFFNVLAF
                     RTGEILNYRNVSEETKTDNKTVKKYIEYLESAFLIKVLHRVDVTAERFKNITQFKVYL
                     TNPSLFIGLFGKISESDERFPHLVETAVFAQYLHREHQYLRYASWNRNGKAGEVDLIQ
                     LSPNFRKAYRALEVKWSDSPKSDKLKHFMLKNSIQKGIITSKRTFNDSDDLLVVPNSI
                     YAYVVGKNALDDQYGE"
     gene            complement(11490..12455)
                     /locus_tag="PK742_02880"
     CDS             complement(11490..12455)
                     /locus_tag="PK742_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU75562.1"
                     /translation="MGDTVQAPGNSIMVIYQDKKGVYWFGSWETGVYRYDGKSLVHYT
                     TEHGLPNNSIDEIKEDGSGNMYFTSCAPKSTIVSFDGNTFTKLSPVLDNAWKHQPGDL
                     WFRHAYQMEKVYRFDGDTLHVLTLPKPPQLSVPFEIYSIYQDRKRNIWFGANPVGVCR
                     YDGNSFDWITEEDVTEFRDEGANGVRSVLEDPNGDFWFNTEYRYSVYDSATLASNRFY
                     TRHESIGGLDGKEDSALDEYLSSVTDNNGDLWFVTYRDGVWKYDGTDITHYPVQVNGK
                     DITLFSIYKDNQGDLWLGSHENGAFRFNENTFERFSPGSALPQTN"
     gene            complement(12700..13233)
                     /locus_tag="PK742_02885"
     CDS             complement(12700..13233)
                     /locus_tag="PK742_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75563.1"
                     /translation="MLNDKYSSTIILCLIDRVVDYLNNGFTILLESDLVGLLMHSYLS
                     DSNLDAKKIHIDTRIKQSNDAQKIDFAIGEVQYSDFSRPFIQPTIVIELKAIPEFGFT
                     QSQKSKRFQYVLSDIEKLKKLNLSLSRWIVIFDGDNYFNNEKMKKIIQHRNEQDRGIE
                     MVVIKKENQKWTKFYYT"
     gene            complement(13684..14517)
                     /locus_tag="PK742_02890"
     CDS             complement(13684..14517)
                     /locus_tag="PK742_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75564.1"
                     /translation="MKAFVLMPFSEEFDDVFHIGIKETAEKEDVVAYRLDEELFDDGM
                     LDKIYSEIESTDFIIADLSNRNSNVFYELGYAHAIGKLCILITQYAENIPFDLKHRRH
                     IVYGSKLTYLQEQLRSNIQWAKNEIEAQKHNPFQIELKFDGNLEATDEYAQANIDFLI
                     DIENKSNRVSPEIQGIYIHSAKQWVIKQNGKKVPSRKSDLKLYSFKYSLSFETPKIPR
                     KGFTQIELNATRVLAYAFNGDEIKDSYTVQGSLFIEVVTNKGSYSSKLPISIYMDTLP
                     F"
     gene            complement(14753..16288)
                     /gene="gpmI"
                     /locus_tag="PK742_02895"
     CDS             complement(14753..16288)
                     /gene="gpmI"
                     /locus_tag="PK742_02895"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQU75565.1"
                     /translation="MPQSRKTMLIIMDGWGHGKNAAASAIAAARTPNVDSYYKLYPNT
                     ELRTDGLFVGLPEGQMGNSEVGHLNLGAGRIVFQELQRIFNAIDSGELAQNKILQAAF
                     EQAKTGGKLHIMGLVSDGGVHSHTRHLKALCQLAIEAGVDKNKLFVHAFTDGRDTDPK
                     SGVKYVGDLDAFLKSNGGRIASVVGRYYAMDRDKRWERIKKAYDLLVHGTGQAFTSAT
                     EAIEASYAAGVTDEFIEPAVLTDENGQALATIAEGDTVICFNFRTDRCRQITRALTQE
                     DFADHDMHTIPLHYVTMTRYDHSFKGVEVIFENEDIVDTIGEVLERNNKKQIRIAETE
                     KYPHVTFFFSGGREKEFTGETRIMRDSPKVATYDLQPEMSAYALRDAIIPELEKGEVD
                     FVCLNFANADMVGHTGVFEAAVAACEAVDTCVGDVVNTAISHGYDVLLTADHGNSDLI
                     INEDGSPNTAHTTNPVPLFYISSDPHSTHLKPGKLGDLAPTLLTLMGLDIPEAMTGEV
                     LLG"
BASE COUNT         4294 a         3612 c         4027 g         4691 t
ORIGIN      
        1 agggagactc cgggtcaagc ccggagagac agttcaacag gagtattgtc agtagtctct
       61 tacctctcac tactcaatac ccactacctt cgtcccatga cacccatcat ccagaccata
      121 atgctgcacc gaaaacaagt ggacgtgctc cgtctggaca agatgaccac tggaggtatc
      181 ggcaacaaat actacaaact gagatacaac ctgctcgcag caaaggagca gggcctgaaa
      241 tctatcctga gtttcggcgg cgctttttcc aatcacctgc atgcattatc ggccatcgga
      301 aaacaggaag gctttcaaac gatcggtgtg gtcagggggg aagatgatcc cgacaatcct
      361 accttgcagg atctgcgtaa taatgacatg cacctgcatt ttatcagcag acaagactat
      421 cggcagaaga atgatgcttc attcattcgt tcacttgaag aacgatatgg atctttttat
      481 ctgcttcccg aaggaggcag taattccctc gccgtgcagg gatgcagaga gatcctgcaa
      541 ggagtgaaac aagactatga ccacatactg gtggctgttg gtacaggtgc taccgttgct
      601 ggtatcgtag ccacacctga gttgacttcc agagtgacag gcatagcagt gctgaaagga
      661 gaggatggcc tgagtgaagt ggtgcaaaaa ttcatcgaac cctatgaaat aaacacaaca
      721 tgggatatct cattccgcta tcacctgggt ggctatgcca gatacaacgc agatctcatg
      781 gagtacatcc ggaatatccg ggacgaaaat gaactggaaa cagaccctgt atatacctct
      841 aaagtggtgt gggcatgtgc tgatatgctg gaaaaaaatg agatcagaca agacgaaaaa
      901 gtgctgctca tccataccgg tggcatgcag ggatggacgg gctggaatta caggtttagg
      961 attagctgat agttatctgg tttttttgag aagttgtcac cccggtttgt gaggaacgag
     1021 caataccggg gtctgtctgg aaatgaattg cgcctcgctc catgtgattc cagatcccgg
     1081 tattttgccc tcattcttcg gcctaaaacc cccgcctgcc gtcaggcagg gggatgacaa
     1141 ttcactttgg agttattgaa caattaccga agtaagagaa tacttgtaac ttaatttgga
     1201 ggccttatca atggatgagt cttcatgagc gatgttcaac ccttactgct caccactctt
     1261 tgctcactgc tttactgcca ctgctcctct gctacccgat acgaatcctc cccactgacc
     1321 ttcatcacca accgttccgg cctcatcacc tatatttgtt gcacacaaaa gaatcattat
     1381 ggattttatc agtaagtata ttgaaacgaa caaggaacgc atgctggacg agctgctgga
     1441 ggtactccgc atcccgtctg tcagtgcaga tccgaaatac aaggccgatg tggaaaagac
     1501 cgcacactgg atggccggaa aactcaaagc cgcgggtgca gacaacgtag aggtttgccc
     1561 aacagaaggt caccccatcg tctatggtga taagctcatc gacccttcca aacctactgt
     1621 attggtgtac ggtcactacg atgtacagcc cgccgatccg cttgagctgt ggaactcgcc
     1681 gccctttgaa ccggtggtga aagacggaaa gatctatgcc cgcggttcct gcgatgataa
     1741 aggtcaggtg tacatgcatg tcaaagcatt cgaagccatg atggccaata atgcgcttcc
     1801 cgtaaatgtg aaattcatca tcgaaggaga ggaagaagta ggttcagcac acctcgagac
     1861 cttcatcaag gagaatactg aaagactgag ctgcgatgtc attctcatct ctgatacttc
     1921 catcgtagcg aatgatgtgc ccagcatcac aacaggtctt cgtggtctct gctatatgga
     1981 gatcgaagtg acaggtccca accgcgacct gcacagcggt gtgtacggcg gtggtgtggc
     2041 caatccactt aacaccatct gcgatatgat ctcttcgctc attgatgaga aaggacatat
     2101 caccattgat ggattctatg atgatgtatt ggtggtaagc gcagaagaac gtgctgaaat
     2161 ggccaaaaca ccattcgatc tggaagaata caagaaagac ctgggcatag gagaggtgaa
     2221 aggggaagac ggatatacca cactggaaag agtatccatt cgtccaacac tggatgtaaa
     2281 cggtatctgg ggcggctaca ccggcgaagg atccaagacc gtactgccgt ccaaagccag
     2341 tgccaagatc tccatgcgcc tggttccaaa tcagtcgccc gataaaattg cgaagctgtt
     2401 cgaagcgcat atcaaaaaga tcgctccgcc gtatgtcagt gtgaaagtga tcgctcatca
     2461 tggtggtgag ccggtggtgg taccattgga ctttgctccg atgcaggcag ccatgaaagc
     2521 catggaacaa tcgttcggca tcacaccgat accgaccaga gaagggggca gcatccccat
     2581 cgtagcgctc tttgaacagg tgctgggtgc caagagtatc ctgatgggat tcggcctcaa
     2641 cagcgatgcc attcattcac ccaatgagca ctacggattg ttcaattact acaagggtat
     2701 tgagaccatt ccgtggttct tcaagtatta tgcggagatg aattagtatt gagtatagag
     2761 tatagggtat tgagatatcc tttaataatg ataagctccg gtctgatggc cggagctttt
     2821 ttgttaggac agaacagcgg tccgaaagtt tttttgtaat atcaggtaaa attttttgcc
     2881 atgtctgcaa aacctgttct gctgatcctt tccttgatca ttaccggagg tattcgttca
     2941 atggctcagc ccatcattga cctggaactc atcaccaccg gatttaacga gcctgtagat
     3001 atagccagtg ccggcgatga gcgtttattc atcgtaagca gacatggtac cattgagata
     3061 ctgtatcccg acctttcggt ttctactttt ctggatatag atgatcgtgt gggttcgggt
     3121 ggcggcgaac agggacttca gggattggtt ttccatcccg actatgcttc caacggcttt
     3181 ttttatgtca attatacaga cacgctgggc gatacacata tttcccgttt cagcgtgagt
     3241 gccggcgatc ctgacctggc agatcctgac agtgagctcc tcctgatata tgcagatcaa
     3301 cctgccaata accacaacag cggcgacctg aactttggtc ccgatggtta cctgtggtta
     3361 tgcaccggta atggtggcgg accagccaat ggcaatgcac aggataccac cagcatactc
     3421 ggaaaagtgt tgcgcattga tgtggatgga gggtctccct atgccattcc tgccgacaat
     3481 ccctttattg gcgaacccgg tgtagatgag atgtgggcac tgggattgcg taacccctgg
     3541 cgtttcagtt tcgaccgact gaccggcgat ctgtggatag cagatgtggg tggtgccatc
     3601 aaagaagagg tcaatttccg gcctgtttct tccgggggcg gagagaacta cggctggcaa
     3661 tgctatgaag cttcttatca gtcggcacta tgcgatacat ccatcgactt taccttcccc
     3721 atttttgaat acaaccacaa tccttttaca ggtggctatg ccattaccgg aggatttgta
     3781 tatcgtggaa atgcttatcc gcatctgtac ggatattata caactataga ttatgttacc
     3841 ggcaatgcat gggccttgca cagcgatggt atgggaggat ggctggtgga tacgcttggt
     3901 tttctggttg atcacatcac ttcatttgga gaagaccaga acggcgagat ctatgcctgc
     3961 gaaaaagatg ccgggaatat ttatcgcctg aaagatctct gcggaagttt tgctctgacg
     4021 tcagacatta ccgatgagac ggcattgggc gtttcagatg gcagtatcga tctgaccgta
     4081 acaggcggga atgcaccata tagcatcagc tggagtacgg gtgcttcttc tgaagatctg
     4141 agcggtctgt ctgcagatac ctacaccgta accgtgaccg atgatctggg ttgtgtgcag
     4201 accggcgtgt atgaggtggc acaattgtgc ggcccggcta ttgatgtgca ggtgtcagac
     4261 atcaccacca gttcggccac catcagctgg agcccttccg gtgcgggttt gtacaaagtg
     4321 cagtacatca ccatgggtgg aacaccggga cagatcacta cgcctgatac ctctgtaacg
     4381 ctgagcggac ttgatgccgg tgcagtatat gtgttccggg tgcggaataa gtgcagcgga
     4441 accaccgcaa cctacaccgc cagcggtgct ttcatgaccg atccgttgcg tgatggtatg
     4501 gtatccggcc tggaagtttt tcccaatcca accaccggtt tgttatacct gcagtctgat
     4561 atgcaggata cacctgttat gatcagagac gtatcgggta atccggtcat gcaatacagt
     4621 gtatgccctg aagtgatcga tctgagtggt ttgcccaatg gcatatatat actggagata
     4681 cacggaagat cgcaacagat cattttgcag cattaaaagt gtgtgtcggt ctctgttagg
     4741 gtttagtcac ttgtacaagg tgacctttat caccaggttc tgatctaaag cacaatacct
     4801 tacatgtact tagtgcagag acatgtttgt tctgacaggc agggtttgct gtcatgttcc
     4861 tgatatacat ggaataaaaa taatataaat gaacaggtat ttgcatttaa ttgggtgtaa
     4921 aggaactaca cctttttttt gaaggttgtt agtttatatc cagttgtcaa cggatgtata
     4981 taggatgcat gtcttacaaa cgaagttaaa gatgaaagga agttgaatga agcaactcac
     5041 acctttcatt tttaaaacaa ataacatgaa aaagatcaaa ttactaggat tgatcatcat
     5101 tgcttatgca acaagtgcct ttgcccagat gggtgtgatc aatgaagacc tcaaaattct
     5161 ttcggacgat gctgccaccg gtgattcttt cggacagtct gttgtgctag acggtggaat
     5221 gatcctgata ggagcacctt ttgacgatga caatggcctt aattcaggtg ctgcttatat
     5281 tgtggatgct gcaacaggcg cacaactttt caagctgctt cctgatgatg gagtggccgg
     5341 agctgaattc ggatatgctg ttgccatgaa agacggagta gctgccattg gtgccagggc
     5401 tgatactgag ttaggtgcag gtgccggtgc agcatatctg tttgatgcag caacaggcac
     5461 acaactttat aaacttgttc cggatgattt gtcggcaggc gatgagttcg gcaatgccat
     5521 tgatatagat ggcggtgtgg tagcagttgg tgcatggcgt gcagatgagt ttggcgaagc
     5581 ttccggagct gcctacttat ttgatgtcgc ttccggcaca caattggaga aattgcttcc
     5641 cgatgagggc aataactttc agaccttcgg tgtatccgtt gctatagatg ccggtgtggt
     5701 agctgtgggt gcacgcacct tttttgtatt gggtgatgga tatacgtttg ccaaggcttt
     5761 tctgttcgat gtatcttccg gcgacctgct gaacaccgtt caacccgata ttcttaacta
     5821 taacggtgat ctgggcggtc attttgctga ctgcctggat atgaaagacg gactgctggt
     5881 agtgggaaca cccaaccgaa gtgtgttctt cgacttttca ggcgccatct atattttcaa
     5941 tgtcggcaca ggagaacagc tgcatttcat atatcctgaa gatggacacg acagagacca
     6001 tttcgggatc tctgtatctc ttgataacga tgtggtaaca attggagctt ttgaagatga
     6061 tgacagcggt tttgatggtg gatcagcata ctattttgat gctatatccg gagaccagat
     6121 cagcaagctg ctggcaagtg atggtgaagc atttgatcgt tttggtgttt ctgtttctac
     6181 acaggacggg ctgactgtga tcggtgccaa gcaggatgat gatggcggag ataatgccgg
     6241 tgcagcttat ctgtttggtg atatttctgc ctgtacaggt ttttctctga tggcaaacat
     6301 tactgatgaa acggctgatg gtctctctaa tggcagtatt gatctgacca taacaggagg
     6361 aactgaacct ttcatgatca gctggagcac cggtgatttc acagaagatc tgagtgcact
     6421 cgaggcaggg tcctattcgg taactgttac agatgatttg ggatgtatgg caaccggaat
     6481 atatgaagtg gcacagttgt gcggcgctgt taccggtctg accgtttctg atatctctac
     6541 gaataccgca acgatcagct ggaactcttc aggcgcatcc atttataagg tgcagtatat
     6601 aactacggga agttcacccg gtgtcgtgaa cactacagat accacggtca ccctgactgg
     6661 tttgttgccc ggatccaact atgtatttcg ggtcagaaat aaatgtaccg gtactacaac
     6721 aacttatacc gccagcggtg ctttcatgac cgatccgttg cgtgatggta tggtatccgg
     6781 cctggaagtt tttcccaatc caacaaccgg tttgttatac ctgcagtctg atatgcagga
     6841 tacacctgta ttgatcagag acgtatcggg taatccggtc atgcactaca atgtttgccc
     6901 tgatgtgatc gatctgagtg gtttgcccaa tggcatgtat atactggagt caggcagtga
     6961 aagaatgatc gttttggtac agcactgatc atcgtcactc ataaaatctg tttttaagct
     7021 cgtttgaaag cgtggtagca ccatccatca tctcatggtg gatggtggct atcaacaatc
     7081 ttttctttac tgcattcatg acatggagac gaacacgtgg atctgtcatg atttgggttg
     7141 aaatgtctgc aaactgctca tcaaaatgac caagatggta agcgctgtta ctgttcttaa
     7201 gatacccctg atggttcagt ttctcgaaag agtaattgat atatccgaat tcttccagca
     7261 catggctgat attgccatct atgatatctg agttgttgtg gtaaatgacc ttttccatcc
     7321 gttccatttc ctggtagtaa cttattaagt catttctgac cccttcatca tttataaggt
     7381 tgatctttcc ggaagatatg agatctgtgt aggtagcatc ctgcactaca aaggttcttc
     7441 tggacagcag gttcgccagt tcagcggcaa atttctgatc aacctgtatc tctttgtttt
     7501 caagggtttc tatcaggtat ttggccgacg aaatataaga tgattcataa tctacctgta
     7561 cctcaatgga cttttgctgt gcatcaatgt ctttaagtat gttttggata tagatggact
     7621 cggcccgttc gtttttggct gcttcatttc ggagattgat ctgaactgct atcaggatac
     7681 cgatgaccag caggatgatc tcgcccactg catacaacag gtagcggcct atgcggcttt
     7741 ctgccataga gttttgcctg aatgttctta agaatttggc cattgaatgg agtaaatatc
     7801 aagatacgat ttctttaagc attagatgtt tgccttattc cgaagatcaa caaatacccg
     7861 atcatccaga actcgccgat ggtggccgga atgacgagaa gttctgtcgc cgtaccatcc
     7921 atgcccaggt actgaagaaa agtgctgagc agataaccgg cacctcctat gataagtgtt
     7981 ctgcccagcc agaggagcat acatgtagaa ctggtcacga tgtatcccat cggtatgagc
     8041 cacaggccga agaacaaact gcctatgccc caggcattgg ttaccagact gctcaatgtg
     8101 gtgatgctgg ccagaacacc atccgttgca tcagtggttc tccaggcgac ctcgagtgcc
     8161 gtgcccatgg cgatggcaga gatcatgatg gccactgagt tgaccatccc ccagatgcct
     8221 aaggtccagg cgccccactc gttgatgcct ttgaacagcc ggtaaaacca tacagctgtc
     8281 agagcttgtg agatgatgat gataaattcc agcaccagcc gcaccctggc cagactctcc
     8341 tgttcctgca ggttggtcag cgtttgttgg gggtcatcaa caaataactg tggatggaat
     8401 accataaaac caagcagccc ggatatggcc atcagcagat accaaatgcc ggtgaggcgg
     8461 gccgttttct tcagttggga gtgtgtggtc attgtgattt atcaggtgta tgagatcaat
     8521 gatttttcca ttcgattccc agccagtcca gcacattctt atgccagatg tcctttcctt
     8581 cctgttcagt gaggatacct tttttcactg cataatccac cacccttccg gggtaggagg
     8641 taccaatgcc ttccatttcc cccagcggat aagggtcatc caggcccatc atgatctggc
     8701 tgcttcccac tcttttcttg agcagttcaa gtgtgtatga gtcatgcacc agggtatcga
     8761 agtagatgtt ctgatggccc agtgtctttc gcggatcctg cagacccctg aacagatccg
     8821 gccgaccatc gaagccctgt atccgcctgc catagcttgc aattcccagc atgccgccat
     8881 gggcaaagct ggtgcgaata tgggggaagg tatcaggcag accgcggagg gtatagatat
     8941 gcaaagtatc ggcacactga gccatcatcc agaccaggtg aaaacgccaa taggcatctt
     9001 tcagggcgat catcttttcg ccatcgtaag ggtggatctg gatggccagg ttgtattcat
     9061 tggccatttc gtagatggga tccacatcag cctcagcggt tgacagccac tctccctgtt
     9121 tgttgaggaa atgggtgggc agacagagca gtttcagatg caggtctttg acacagcgtt
     9181 cgatctcgcg gagggcctgg tcaatgtaca ggggctggac cacgaaaccg caggtgaact
     9241 tgtccgggtg atcagcctgc acctgtgcgt tgaaatcatt ctgaaaacgg atggcatcat
     9301 ggcaatcggt ttcctcccag ccattgcagt agagctgtga cagacatagc aagacttcgt
     9361 gatcgatctg attcaggcgc atccactcta gtttctcttc cagaaagaaa ctggggtggg
     9421 tcacgggtct ggaccagtcg ccttgtctca tgaatcgttt gtcttcatcg atccagaaca
     9481 gctttttttc cttcatgaaa gaagggatct gtgaaggttc agggagcaga tgtccgtgtc
     9541 cgtttattct cattccaaca catttgggtt tgcaatttaa ccatattgcg ggcatagcag
     9601 attttagaga acgaatcagc acgataagaa cctgcgattt atctttctaa ggtcagatgc
     9661 cagggtcagc tggtctttta tgaagtgata ctttaatggc gctctgatac ccgctttcaa
     9721 tttcttttca ggatcttgtt ggttcaacag gcagacgatg gctcgttttc catcgtattg
     9781 gaatgtgcgg tcaataacca ggctgcatgt atctgccagt ctgcaggtcc ataaaaaata
     9841 aacggcatac gagtatggca cttttttgta ccccggtccg tgtaaacctg tccgtcaggt
     9901 gaaaatagca tcgattaagg tacagatata caatgagtta caacaggata caccaattta
     9961 gggatgaaga tgcttaaatc tgcattttat gcaataaatg ggattgtaat acttaaattt
    10021 gcagtatggt gtttagtgat aatattctgg aaaatcggat caaatcagat catacctggt
    10081 gggaaacaaa gcaaattcca ttctatacgg agcttaacaa aaggagatat ttcacaccat
    10141 tttacgggtt aattcagcaa tcagccttac atcgggcggt ggtattgatg gggcccagga
    10201 ggatcggtaa aacggtaatg atgtatcagg ccatacagga tctgcttgat agtggaatag
    10261 agcctaagaa aattctgtat ttcagtttag atacccccat tttcacgaat gtttctttgg
    10321 acgaattgat attcagggca cttaccccaa atgatgagtc tattgaagat tgttttattt
    10381 tttttgatga gatacagtat ctgaagaatt gggaaataca tcttaaatct cttgttgaca
    10441 gaaacagaag aacaaaattc attgccagtg gtagtgcagc agctgcatta agaatgaaga
    10501 gcatagaaag tggtgccgga aggttcagcg atttcttttt accgccacta acgtttgccg
    10561 agtacattga tctgaatggt tataacgcat ctctggttaa gatcgaggtt gatttcggtg
    10621 gccccagaag attctataag acggaagata tagaacaatt caacaggcac ttcacagact
    10681 acatcaattt tggaggcttt ccggaaattg cactgaacaa ggaggaagta aaaaatcctg
    10741 aacaggttat acaaaaagac attacagaca aggtcatcat gaaggatctg cctggtctgt
    10801 ttggcattga aaacacgtta gaactccagc agttttttaa tgttcttgct ttcagaaccg
    10861 gtgagatatt gaattacaga aatgtatcag aagagaccaa aacagataat aagaccgtca
    10921 aaaaatatat agagtatctg gaatctgctt tcctgatcaa agtactgcat cgtgtagatg
    10981 taacggctga aagatttaaa aacatcacac aatttaaggt ttacctgacc aacccaagct
    11041 tatttatcgg attgtttggt aagatctctg aatcagatga acgatttcca cacctggtgg
    11101 agacggcagt ttttgcacag tatttgcacc gcgaacatca atatctgcgt tacgccagct
    11161 ggaataggaa tgggaaggcc ggagaagttg atctgatcca gcttagtcct aatttccgga
    11221 aagcataccg ggcacttgag gtaaagtggt ctgattctcc gaagtctgat aaattaaaac
    11281 atttcatgct gaagaacagc attcagaaag gaataattac cagtaaaaga acctttaatg
    11341 actctgatga cctgctggtg gtgccaaaca gcatttatgc gtatgtagtt ggaaagaatg
    11401 ctttagatga tcagtatggt gaataacagc tattcatact tcctgatttt gtcagcgttt
    11461 ttaacgaact cagattcctg aagtgtccct taattggttt gtggaagtgc gctccctgga
    11521 ctgaaccttt caaatgtatt ctcattgaat ctgaatgcac cgttctcatg agagcccagc
    11581 cagagatcac cctggttatc tttgtaaatg ctgaacaggg tgatgtcctt cccatttacc
    11641 tgaacagggt agtgggtgat gtccgtgccg tcgtatttcc atacaccgtc acgataggtg
    11701 acaaaccata gatcgccatt gttgtcggtt accgacgaca aatactcatc cagagcactg
    11761 tcttccttcc cgtccaggcc acctatactt tcatgcctgg tatagaatcg gttgcttgcc
    11821 aatgtagcgc tgtcatacac actataccgg tattcggtat tgaaccagaa gtcgccgttc
    11881 gggtcttcca gcaccgaacg cacgccgttg gctccttcat cgcgaaattc tgtcacgtcc
    11941 tcttcggtga tccagtcaaa ggagtttcca tcatagcggc atacgccgac cgggttagcg
    12001 ccgaaccaga tgttcctttt ccggtcctga tagatgctgt agatctcaaa aggaacggaa
    12061 agctgtggtg gttttggtag tgtcagtaca tgcaaggtgt caccatcaaa acgatatacc
    12121 ttttccatct gataggcatg tctgaaccac agatcaccgg gctgatgctt ccaggcatta
    12181 tccaagacag gcgataattt tgtgaatgta ttaccgtcaa aactgacgat cgttgacttt
    12241 ggagcgcaac tcgtgaaata catattacct gatccgtctt ctttgatctc atcgatactg
    12301 ttgtttggca aaccgtgctc tgttgtataa tgaaccagcg acttaccatc gtatctgtac
    12361 accccggttt cccagctgcc aaaccagtat acaccttttt tgtcctggta gatgaccatg
    12421 atgctgttgc cgggtgcttg cactgtgtca cccattacgg actgttcatt tgaacttttc
    12481 tgatcagcgg tctgtccgct gcaggaaata aggagggtta aaatggaaag aagaagcagg
    12541 ttgggtttca tggtgtttgt gctgatctga gccaatgccg ataggtttat caataagatt
    12601 taaaattcgc agctattcct catcagggct tccaaaagta tgttagcaaa taataataaa
    12661 gaaaaaatgt taattatgct aaatgtagct tatcaaattt tatgtataat aaaattttgt
    12721 ccacttttga ttttctttct ttattacaac catttcgata cctctgtctt gttcgtttct
    12781 atgttgtata attttcttca ttttttcatt gttgaaataa ttatcaccat caaatattac
    12841 tatccacctg cttagtgata ggtttaattt ttttaacttt tctatgtcgc ttaatacata
    12901 ttgaaatctt ttactttttt ggctttgtgt aaatccaaat tctgggatgg ctttcaattc
    12961 tataacaatt gtaggctgta tgaaaggtct tgaaaagtct gaatattgca cttctccaat
    13021 tgcaaagtca attttttgcg catcatttga ctgtttaatt cgagtgtcta tatgaatttt
    13081 tttagcgtct aaattggagt cacttaaata tgagtgcata agaagaccta ctaaatcaga
    13141 ttctagcaaa attgtgaatc cattatttag gtagtctaca actctgtcta ttaagcaaag
    13201 gattattgta ctactatatt tatcattcag cacgttattt agatttgaat tttgatatta
    13261 tcaacatgag gctgccaacg atatatagaa gtgatctaaa gaaaaatgtc caatcaagtt
    13321 gccgatttac actttctatt tcttttccag gctcatcaaa gtattcccaa ttatttacga
    13381 tatcggagaa gtctgcagaa gtatatgttc cgctgtggtt tagtttactc tgagaatata
    13441 gatcagctgt tatcatgtaa attttgtcta attttttgtg tatgtgataa ttgttaaggt
    13501 atgtggattg attagaaagg tcggtttcaa acatttgcca tccaaatgaa aacaaaataa
    13561 aaattaggcc tgttccttca aggtttttca agatagattt cattctatat ataatttgct
    13621 ttatgatttt cattttgact atttaatttg tagagtagtc ttcgttgtaa ctggtttcat
    13681 agtttagaat ggtaatgtgt ccatgtatat cgaaatgggc agtttgctgg aatatgatcc
    13741 tttgttggtt actacttcaa taaaaagact gccttgtact gtataactat cttttatttc
    13801 atctccatta aaagcatatg ctaaaactct tgtggcattt aattctattt gggtaaaacc
    13861 ttttcttggg atttttggtg tttcaaatga aaggctgtat ttaaagctat aaagctttag
    13921 gtcagatttc ctagaaggga cctttttccc attttgtttt attacccatt gttttgcgga
    13981 gtgaatgtag attccttgaa tctctggaga aacacgattg gatttatttt caatgtcaat
    14041 aaggaaatct atattggctt gtgcatattc atcagtagct tctaaattgc cgtcaaattt
    14101 caattcgatt tgaaatggat tgtgtttttg agcttcgatt tcatttttgg cccattgtat
    14161 atttgaccgt aactgttcct gtaaataggt gagtttgcta ccataaacaa tgtgccgtct
    14221 gtgctttagg tcaaatggaa tattttctgc atattgtgta attagaatgc aaagtttacc
    14281 aatcgcatga gcatagccga gttcgtagaa tacattagaa tttcggttag ataaatcggc
    14341 tataatgaag tctgtagatt caatttctga gtatatttta tcgagcattc catcatcaaa
    14401 aagttcttca tcaagtctat aagcaacaac gtcctctttt tctgcagttt cctttattcc
    14461 aatgtgaaac acatcatcga attcttcaga gaaaggcatt aatacaaagg ctttcatttc
    14521 aatttattta atgatttccg ttatgccact aatgtagcta caaaagctaa tgttttgcta
    14581 gtatccagag ccgaaggcac cacgattctt cccagcccgg attgcagtgg cagcgccctg
    14641 gccagttgag agtttggtca aataaggtag gagatattca gccggggact acaacgaaaa
    14701 gccggaccaa agtgactggt tggcaggagg gggattgctc catattattt tctcatccca
    14761 acaacacttc tccggtcatc gcttccggaa tgtccaggcc catgagggtg agcagggtgg
    14821 gggcgagatc gccgagcttg ccgggtttca ggtgggtgct gtgaggatcg ctgctgatgt
    14881 agaacagcgg cacggggttg gtggtatgcg ccgtgttggg tgagccatcc tcattaatga
    14941 tgagatcgct gttgccgtga tctgctgtga ggaggacatc ataaccatgg ctgatggcag
    15001 tattcaccac atcgcctacg caggtatcta cggcttcgca ggcagccacc gcggcttcga
    15061 acacaccggt gtggcccacc atgtctgcat tggcaaagtt gaggcataca aagtccacct
    15121 cgcccttttc cagttcgggg atgatggcat cacgcaacgc ataggcactc atctcgggct
    15181 gcagatcata ggtggctacc ttgggagaat cccgcatgat gcgggtctcg ccggtgaatt
    15241 ccttttctcg tccgccggaa aagaagaagg tgacgtgcgg atatttttct gtctctgcga
    15301 tgcggatctg tttcttgttg ttgcgttcca gtacctcgcc gatggtatcc acgatgtcct
    15361 cattttcgaa gatgacctcc acgcctttga agctatggtc gtagcgggtc atggtgacat
    15421 aatgcagcgg tatggtatgc atgtcgtgat cggcgaaatc ctcctgggtg agtgcccggg
    15481 ttatctgccg gcaacggtcg gtgcggaagt tgaaacagat gacggtatcg ccttcggcta
    15541 tggtggccag ggcttgacca ttttcatctg tcagcacagc gggttcgatg aattcgtcgg
    15601 tcaccccggc ggcatagctg gcttcgatgg cttcggtggc agaagtgaac gcctggcctg
    15661 ttccatgcac gagcaggtcg taggcttttt tgatgcgctc ccagcgcttg tcgcgatcca
    15721 tggcatagta tcgtcccacc acggaagcga tgcgaccgcc attcgatttc aggaatgcat
    15781 ccagatcgcc cacatacttc accccgcttt tggggtcggt atcgcggcca tcggtgaagg
    15841 catgtacaaa gagtttgttc ttgtccacac cggcttcgat ggccagctga caaagggctt
    15901 tgaggtgacg ggtatgcgag tgcacaccac catcactgac aagtcccatg atatggagtt
    15961 ttcctcctgt tttggcttgt tcaaaagcgg cttgtaaaat tttgttctgt gccagttcgc
    16021 cggagtcgat ggcgttgaag atgcgctgca gttcctgaaa gacgatgcga ccggcaccga
    16081 ggttgaggtg acctacttcg ctgttgccca tctgtccttc gggaagacct acgaagagcc
    16141 cgtcggtgcg cagttcggtg ttgggataga gcttataata ggagtctacg ttcggtgtgc
    16201 gggcggcggc gatggcactg gctgcggcgt tctttccgtg tccccagccg tccatgatga
    16261 tgagcatggt ctttctgctt tgtggcatgt tgtaaaggta gtgagtattg ggtattgggt
    16321 agtgagtagt gagtagtgag tattgggtat tgggtagtga gtattgggta gtgagatgtg
    16381 aggggtgagc tcttatccgt gaaatctgtg tttcagcatt ttaacgcaaa ggcacaaagg
    16441 aggcgcaaag gacgccaagg caacaggact ccccatccgt gaaatctgct aaatccctgc
    16501 ttgcagcagg caagcgtgtt tcagcatttt aacgcaaagg cacaaaggaa acgcgaagga
    16561 cgccaaggaa acaggactcc ccatccgtga aatctgctaa atccctgctt gcagcaggca
    16621 agcg
//
LOCUS       DAOMAX010000028        16560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_375_length_16560_cov_5.447561, whole genome shotgun sequence.
ACCESSION   DAOMAX010000028 DAOMAX010000000
VERSION     DAOMAX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16560
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_16560_cov_5.447561"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            977..1939
                     /locus_tag="PK742_02900"
     CDS             977..1939
                     /locus_tag="PK742_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75566.1"
                     /translation="MSNTFNVGSGDEFQVSSTGDLVRINDVGYTWPDDDGDANQVLQT
                     DGSGNLDWETVVTDVQVFTGTTTLWAKPTGAVSVTVICIGGGGGGGSGGVTGDMSGGS
                     GGGAGGMSEMTFNAANLDANVLAYAGAGGTGGAAVSSGDGKDGGNGTKSFFGDYLKAS
                     GGNGGNGGSSTGAAGGSGGPGLKSQGGSGASADPADQGDDAGNSNYASLGGGAGGGIS
                     SGGTAYSGGDGGGNIQFMNSSPAAGSGGTSGGSGGNGTAESGNYLGSGGGGGAASASA
                     TAGSGGNGASYGAGGGGGGAAGSGHTSGAGGDGANGVVIVITYF"
     gene            1962..3470
                     /locus_tag="PK742_02905"
     CDS             1962..3470
                     /locus_tag="PK742_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQU75567.1"
                     /translation="MKNLIQLICMAFLAAVINPASAQNIFPDSGFVGIGTTTPELLLD
                     INSPDGHASIGLGTSTIYNGPAGIGFGEGVLTSNLDGDYNTAIGWLVLTDNLIGSYNT
                     AIGAEAMRYNKSGSQNVAVGYLSLKYNYFGESNVAIGNYSLFSNKSGDNNVSIGSNAM
                     LSNKIGNENTAIGSSAMSDNTKGNENTAIGRASLSNNTLGNSNTAIGALALNVNKTGN
                     YNIGIGYNATTTGNDFENSIAVGYIAVVDASNKVLIGNSSVSSIGGEVDWTVYSDARI
                     KEQVEENVPGLDFINQLRPVTYHYNITTQNQLMGRVDTATWTGKYDIEEQAFSGFIAQ
                     EVAAAAEDLGYEFSGVDASGRLLGLRYATFVVPLVKAVQELDAENTNLEDRISRLEAL
                     LGQQGIQMNESTTDEVQRIFVASNQTQATLSQNRPNPFTGSTSIRYFVPETAEQAIIK
                     IADAAGVSLFMADVKLGNGVLEVDATQLAAGTYSYTLLVDGKVVDTKLMVIQ"
     gene            complement(3658..4737)
                     /locus_tag="PK742_02910"
     CDS             complement(3658..4737)
                     /locus_tag="PK742_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQU75568.1"
                     /translation="MDNVYVAIMAGGIGSRFWPESRASKPKQFLDILNTGETLIQTTF
                     RRFLKIVAKDNIFIVTNEDYVGLILEQIPDVNPANILAEPARRNTAPCVAYVSHKIAA
                     INPDARVVVAPSDHLILNEPKFLEFIGKALQHTETHDHLVTLGIHPTRPDTGYGYIQF
                     TDREAAPGIFKVKTFTEKPNLELAVTFLRSGDFLWNAGIFIWKVEAILDAFDHHLQDM
                     HQAFWQARKMFGHPEEKAAIEKAYSLCTNISIDYGIMEKADNVFVIPASFGWSDLGTW
                     ASLWNNYNRDYWGNAVKGKKVMIYDSKDCMVMAPDDKLVILQGLDEYIIIDQGDVLLI
                     CKKEHEQEIKNITADVKRRLGDRFL"
     gene            4893..7094
                     /gene="recQ"
                     /locus_tag="PK742_02915"
     CDS             4893..7094
                     /gene="recQ"
                     /locus_tag="PK742_02915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HQU75569.1"
                     /translation="MLTSELSLKEALQEYFGFNTFKGNQEAIIQSMLDGRDTFVIMPT
                     GGGKSLCYQLPAVMLEGTAIIISPLIALMKNQVDLVRSYSSNDNIAHFLNSTLSRTTI
                     KKVKADITSGKTKMLYVAPETLKKEENLEFFRGIKVSFIAVDEAHCISEWGHDFRPEY
                     RQIKTMIGQIDAKIPIIALTATATPKVQSDIKKNLDLEDPNVFLSSFNRPNLYYEIRP
                     KRSREQAIKQIVKYIQEHPNKSGIVYCLNRKSTEEIAELLNVNGIKAVPYHAGMEAAT
                     RTQVQDQFLMEEMHVIVATIAFGMGIDKPDVRFVIHFDIPKSIENYYQETGRAGRDGL
                     EGKCIAFYSYKDIEKLEKFMRDKPLAEREMGAQLLAETAAYAETSVCRRRFLLHYFGE
                     GYDQDNCGHCDNCLNPKEKIEGKTFVQTALKVVQAVNENYGIPYLVDLLMGKRTTEIG
                     NYRHDQLEIFGSGKEKDDHFWHSVLRQCLLESLVYKDIEQYGLIRLTDAGRKFNDKPK
                     NIKLSINHDYEAEAADVQVEGSGGKSVLDQTLFSMLVKIRESVAKEQKLPPYVIFSES
                     SLEEMATTYPCNLEELSKIGGVSAGKANKYGVKFAAAIKKYVEENEIERPGDFNTMKQ
                     VANKSVHKIHIIQNIDKKLPLDEIARNRGMKMIELIDEISSIVTSGTKLNLDYYLNDI
                     IEEDLQDEVMDFFREMESGDLKEAFNELKDEGLSYEEVQLLHIKFMSEVAN"
     gene            7122..7889
                     /locus_tag="PK742_02920"
     CDS             7122..7889
                     /locus_tag="PK742_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQU75570.1"
                     /translation="MASNETFEIKLPQFEGPFDLLLFFIERDELDIYDIPISRITNDF
                     LDYIHQMNQLNIEVASEFILVAATLMRIKAKMLLPRKELDEFGNEIDPRKELVDRLVE
                     YKRYKDVLEELGNLEDQRRFKMQRGNIQAEHSVLAKQFSNEMELSAVNLFTLLKVFEK
                     VMERYRHQPEEVEHVVFQYNYTIEEEADNVISFCKSKDRVDFEELFSTCADRHQAIFR
                     FLSVLDLIQLQRIDLLIGEGFNQFWVSYNQFREDDAA"
     gene            7876..8973
                     /locus_tag="PK742_02925"
     CDS             7876..8973
                     /locus_tag="PK742_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQU75571.1"
                     /translation="MTQPEPDIPVSDQAEQLNKQFSYRKVLWVIALSLVLSGYLIFVS
                     FDVDAFRLISWTANSLLLLFLGYILLGIRHLAYMYRIRHLTNKHLSWRQSFEVITLWL
                     FSSAVTPSTVGGAAVAVYLLKKEKLSVGQSATTSMLTVFLDQAVFAVIAPLFTLLVGS
                     EEMFAKDMGCRTQSELPMMRLFHNVETLYYVAYIFYLLVVIGLAWGLFVNAGAFKSFL
                     GRLFSLPFLKRWKAEALRTGDDIVATSHMLKGKNLRFWLKAVGSTLLAWLSLFLISVF
                     IFMAFFDPATLEYLVVMARQTVVWMITLIPATPGGAGVAEISFSALMCDITSPALVPI
                     MALLWRMISFYPYLLMGIFFLPRWMKRVYNN"
     gene            9046..10293
                     /locus_tag="PK742_02930"
     CDS             9046..10293
                     /locus_tag="PK742_02930"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HQU75572.1"
                     /translation="MDNKNLHPESLMMGFGYKPELSEGAVKCPIFQTSTFVFKTAEEG
                     KRFFELAYGLQQAEVNEVPGLIYSRLNNPDLEILENRLCLWDKAEDCAVFASGMGAIT
                     TVMLEFLQPGDVLLYSSPVYGGTDHSINHVLPKWSVGHVEFNASMRKEEIIQKVEQEQ
                     GGKLAMIYVETPANPTNALIDLEMCREIADHFSTDERKVLVVVDNTYMGPLWSHPLQL
                     GADLVVYSATKYIGGHSDLIAGAVLGNRELMTRVRGLRTFLGNMCSPFTGWLMMRSLE
                     TLKPRMETQAANARQVADFLKDHPKVEKLYYLGHLEEGSEQYRIREKQYSSSGAMLSF
                     DVKGGEAEAFRFLNGLKLIRLAVSLGSTESLAEHPATMTHADVSPADKLKHHISESLV
                     RISIGVEHHEDLIDDIRQALENV"
     gene            complement(10349..11098)
                     /gene="lptB"
                     /locus_tag="PK742_02935"
     CDS             complement(10349..11098)
                     /gene="lptB"
                     /locus_tag="PK742_02935"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQU75573.1"
                     /translation="MILRSEGLVKKYKRRTVVNEVSVQVEQGEIVGLLGPNGAGKTTS
                     FYMIVGLIKADAGEVYLDNKPITKLPMYKRAQLGVGYLPQEASVFRKMSVEDNIAAVL
                     QMTKLSRKEQAHKLEELIDEFGLGHVRKSNGDVLSGGERRRTEIARALAVNPKFILLD
                     EPFAGIDPIAVEDIQGIVETLKFKKIGVLITDHNVQETLSITDRAYLLFEGKILKQGS
                     AEELAEDPQVRKVYLGQSFELRRKVRNGSSN"
     gene            complement(11095..12795)
                     /gene="recJ"
                     /locus_tag="PK742_02940"
     CDS             complement(11095..12795)
                     /gene="recJ"
                     /locus_tag="PK742_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQU75574.1"
                     /translation="MDKRWVIRESDAGAVHILQEALGIHPVLCKLLVDRGIDTYDKAK
                     QFFRPQLSDLHNPFEMKGMREAVNRILRASEQGERILVYGDYDVDGTTSVALMFSFLN
                     RFYPNIDYYIPDRYKEGYGISFQGIDYAEDNNCALIIALDCGIKAVEQVAYANSKGID
                     MIICDHHLPGDELPAAVAILDPHQPDCNYPYKYLSGCGIGFKLAQAITLEAGLDEDRA
                     LDLLDLVAVSIASDIVPITGENRVLAFYGLRKMDTNPTKGLKALMEISDVRPPLSISK
                     IVFGLGPRINAAGRLDDARKAVRMLISSTDQFARNHAGDLQGHNMDRKEIDRSITAEA
                     LEMLDNDPLTPERVTNVLFNPAWHKGVIGIVASRIMDHYYKPTIMLTESNGVVAGSAR
                     SVKGYDVYEAISACGDLLEQFGGHMYAAGLTMKPENVEAFKDRFEKVVKSTIRPEQLI
                     PEIEIDAVLNLKDITPGFYKILRQFAPFGPENLPPVFITEELTCNGRSKVVGNGHLKL
                     DVRDRNRFRASGIAFQQGDHYPYVSSGYPFDLAYYLEENEFNGQTNLQLNVKDIRVHA
                     "
     gene            complement(12806..14431)
                     /locus_tag="PK742_02945"
     CDS             complement(12806..14431)
                     /locus_tag="PK742_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQU75575.1"
                     /translation="MKKWIWIIWFLLPVVTSAQDSNRVPGQLIVELFSDARPEQLIAD
                     LQQAGFDCQLAYVCVQALHLYVISIPEEAHEQVFAYCQQHPDVKQLQYNHYLEIRGFP
                     DDSSFDLQWALYNDGSTGGTDDADIDADLAWDLSTGGWTAEGDTIVIAIVDDGFYLPH
                     NDLVFRKNYSEIPGNGLDDDDNGFIDDFDGWNSLDNSDNLPEFSHGTHVSGIAGARGD
                     NALGVTGVNWNVQILPLSIGAGAVIESNVLECYGYVYTERKRYDDSNGTEGSFIVATN
                     ASFGIDFADPNDFPLWCSMYDSLGTLGILSAGATANSNVNIDLVGDMPTACSSDFLIT
                     VNSTNKTDNKSTSGYGSVSIDLGAPGSLIYSTVVGNSWNYQTGTSMAAPHVAGAVALM
                     LSYGCENFLYQYKHDPETAALALKQFILDGVDPLEDLDTITVSGGRLNVYNAMLMLDE
                     YCDTLTAIAQVDEVQFGLYPNPATDYLTIQTNGGYFLYTLADVWGRTCRSGEISGRTT
                     ALVDIRNLPAGIYVCTLRTSAGKPAGSARVVVE"
     gene            14603..15172
                     /locus_tag="PK742_02950"
     CDS             14603..15172
                     /locus_tag="PK742_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HQU75576.1"
                     /translation="MIHFFENLDPVLKIFWYTALPVSIIFLIQTIMTFMGTDGGDGMS
                     ADFDSDLDGADAPFQLLSFRSLIHFLLGFSWSGISLFGTIHNSLLLIVVSALIGGLFV
                     LVFLWLMQQLIKLAENNSFTYQDTLDKVGEAYTPIPGQKAGIGKVLISIKGSTHELEA
                     LTDGDRIESGTPVRVLRLESDRILIVEKI"
     gene            15184..>16560
                     /locus_tag="PK742_02955"
     CDS             15184..>16560
                     /locus_tag="PK742_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQU75577.1"
                     /translation="MVSPVIIVVVAAIVFFVTVSVLVSRYKRCPSDKILVIYGRTGGT
                     SAKCIHGGGAFIWPVVQDYAFLDLKPLSIEANLTNALSRQNIRVDVPCRFTIAISTEA
                     DNMNTAAERLLGLSSEQIQELAKDILFGQLRLVIATMTIEEINSDRDKFLENISKNVD
                     SELKKIGLKLINVNVTDIKDESGYIEALGKEAAAKAINDAKISVAEQEKIGETGKALA
                     DREKDVQIAETHRDRDVKIAITQKDREVSIASAHKDEAIGKAEADRDTRIKTSEANAF
                     AIEGENTAKISIANSDANRREKEAEALRVAITAEKVQQAKALELAYQAEQKAETARSE
                     RERSTQVANVVIPAEISKQKAIIEAQAIAEAVRENAKGEADAIYAKMEAEAKGLYEIL
                     TKQAEGYREVVAAAGGDPTKAFHLLLIEKLPELVKTQVEAIKNIKIDKITVWDSGNDK
                     ESGQGSTAN"
BASE COUNT         4196 a         4061 c         4272 g         4031 t
ORIGIN      
        1 caaaacacaa caccatgaag gttaatttga tgttaagccc cccccatttt acaaactgat
       61 cgtttgtttg ttgctcacat ccatgtgtgg taaccgcatg tttgcacaga catgtgatcc
      121 cgcctgtgaa gacctgagca atctggacgt aacaactaat atcaattcac atcttttgcc
      181 tgatgcagat cggaatttag ggtctgaaga ttccagctgg tcagaaattt atctggaaaa
      241 ggtcattcac tttgatgaag cagaaggata cagcaatgta gatatgctga aactaaacag
      301 caataaattt ctgcacgtta caggaggttc gtatgatggt ttatttcttg gtgaatatgc
      361 aggggatgca gcttcgagct ccaatcatgg aacggtgggt atcggatata aagccttaga
      421 aacagtcact ggttctgcag ggcacaatac ttcggttgga tggtactcat ccagcaatat
      481 taccagcggc ggcaagaata caatgatcgg tagcagagct ggtgagttga ttacaaccgg
      541 atcatcaaat attggaattg gccaaaatac ctactataat aattcagata gcccgcaggt
      601 gaccggtgat ggcaacattg ctatcggcgg tacttcaact actactggtg tgttgggtaa
      661 tgtaacttca ggtgattata atgttgcaat aggttataat tcagttatag gcctaacaga
      721 aggtgttgaa aatacagtaa ttggatattc agctggctca tctattagcg acgggagcaa
      781 taacattttt gttggctcgg aatctgggaa aggtagcttt ccaagccaat caggtaataa
      841 taatattgta atcggtaaca atatttcagt aaatccatca accggtttgg atttaagcaa
      901 caggctaaat attggaaatc tattatatgg cgatttgagt tctggtaaat tcagtgtcgg
      961 caatagcggt tcaacattgt caaatacatt caatgtagga tccggagatg aatttcaggt
     1021 cagttccacc ggcgacctgg tacgtatcaa tgacgttggc tacacctggc cggatgatga
     1081 tggcgatgcc aaccaggtat tgcagaccga tgggtcaggc aacttagact gggagactgt
     1141 ggtaaccgac gtacaggtgt ttacaggaac aactactctg tgggctaaac ctaccggggc
     1201 tgtttcggtg acagtcatct gtattggcgg tggtggtggc ggaggcagtg gtggggttac
     1261 cggtgacatg tccggaggat cgggaggagg cgccggaggc atgagtgaaa tgaccttcaa
     1321 cgctgccaac ctggatgcca atgtactggc ctatgcgggt gccggtggaa caggaggagc
     1381 tgccgtaagt tccggcgacg gaaaggacgg tggcaatggc accaaatcct tttttggcga
     1441 ttacctgaag gcaagcggag gcaacggtgg gaacgggggc tcctcgacag gggcagccgg
     1501 agggtcaggt ggtccggggc tgaagtctca gggaggctct ggtgccagtg ctgacccggc
     1561 agaccagggc gatgatgcgg gcaacagcaa ctacgctagc ctgggtggag gagccggagg
     1621 tggtatcagc agtggcggaa ccgcctattc gggtggtgac ggcgggggca atatccagtt
     1681 catgaatagt tctccggcag caggcagcgg aggtacttcc gggggcagtg gtggtaatgg
     1741 aacagcagag agcggtaatt acctgggctc cggtggtggt gggggcgctg ccagtgcttc
     1801 cgcaacagcc ggaagcgggg gcaacggagc cagctacggc gcaggaggtg gcggtggtgg
     1861 tgctgccggc tcaggacata ccagtggtgc cgggggtgat ggcgccaacg gggtcgtcat
     1921 cgtgataact tatttttaaa tattcttata aatcaagcaa aatgaaaaac cttattcaac
     1981 tgatctgcat ggcgtttctg gcagctgtca ttaacccggc cagcgcacag aatatctttc
     2041 ccgactccgg atttgtgggt attggtacca caacaccgga acttctgctg gacatcaatt
     2101 ctcctgatgg gcatgccagt attgggttag gaacctcaac tatttacaat ggacctgccg
     2161 ggatcgggtt tggggaagga gtacttacca gcaatcttga tggtgattac aatactgcta
     2221 ttggatggct tgttttgact gacaacttaa ttggatcata taacacagcc attggagcag
     2281 aggctatgcg gtacaacaag tccggatcac aaaatgttgc cgttggatac ttgtctctta
     2341 aatacaacta ctttggagaa agcaatgtag ctattggaaa ttattcgctt ttttctaata
     2401 agagtggtga taataacgtt tccatcggtt ctaatgctat gctgagcaat aagataggaa
     2461 acgagaatac tgcgataggt tccagtgcaa tgagtgataa tacaaagggg aatgaaaata
     2521 ctgcgatagg tcgtgcaagt ttatcaaata atacactggg gaatagtaat acagcaatag
     2581 gagcattggc tctaaatgtc aataaaacag gtaattataa tattggaatt ggctacaatg
     2641 ctactacaac tggtaatgat tttgagaact caatagctgt tggatatata gctgtagtag
     2701 atgcttccaa taaagttcta atcggcaact ccagcgtctc cagcatcggt ggtgaggtgg
     2761 actggaccgt gtacagcgat gcccgcatca aagagcaggt ggaagagaat gtgccggggc
     2821 tggatttcat caatcagctg cggccggtta cctaccacta caacatcact acccagaacc
     2881 agctgatggg cagagtagat accgctacgt ggaccggcaa atacgacatt gaagaacagg
     2941 ccttttccgg attcatagcc caggaagtgg ctgctgcggc agaagacctg gggtatgagt
     3001 tcagcggcgt agatgccagc ggtcgcctcc tgggcctgcg ctatgccacc tttgtggtac
     3061 cgctggtaaa agcagtgcag gaactggatg cagagaatac aaatctggaa gatcgcattt
     3121 ccagactgga agccctgctt ggtcaacaag gtattcagat gaatgagtca acaacggatg
     3181 aggtacaacg gatctttgta gcctcaaacc aaacgcaggc caccctttct cagaacagac
     3241 ccaacccttt taccggtagt acctccatac gctattttgt acccgaaact gccgaacagg
     3301 caattataaa aatagccgat gctgccgggg taagcctgtt catggctgat gtaaaactgg
     3361 gcaatggtgt tttagaagta gatgctacac aattggcagc aggtacttat agctatacat
     3421 tgctggtgga tggaaaagtg gtggatacga aactgatggt gatacagtag ggtttgtatt
     3481 ggggttcagc tcttttctcc tctagaccct accgtcccgg ccgggtcagc agaactagac
     3541 caggcatctg atatattctg gtatctcgcc atgtcctgtg gcctgtgaca tggctgagac
     3601 aggttgctat gttttgaatc gggtagggag ataattttcc taaattcctt tccctcctta
     3661 caggaatctg tcgcccagtc tgcgcttcac atcagcggtg atgttcttga tctcctgttc
     3721 gtgctccttt ttacagatca gcagcacatc gccctgatct atgatgatgt attcatccag
     3781 accctggagg atgaccagtt tatcgtcggg tgccataacc atacaatcct tgctgtcgta
     3841 gatcatgact ttcttgcctt tcacggcatt gccccagtag tcgcggttgt agttgttcca
     3901 cagcgatgcc caggtaccca ggtcgctcca gccgaaactg gcgggaatga cgaagacatt
     3961 atcggccttt tccatgatgc cgtagtcaat ggatatattg gtacaaaggg aataagcttt
     4021 ttctattgca gccttttctt ccgggtgacc gaacatcttc ctcgcctgcc agaaagcctg
     4081 gtgcatatcc tgcagatggt ggtcaaatgc atcaagaatg gcctctacct tccatatgaa
     4141 gataccggca ttccaaagga aatcgccgga gcgcaggaag gtgaccgcca gttcgaggtt
     4201 gggcttttcc gtgaaggtct tcaccttgaa gatgcccggt gctgcttcgc ggtcggtgaa
     4261 ctggatataa ccataaccgg tatcgggcct ggtagggtga atacccaggg tgaccaggtg
     4321 gtcgtgggtc tccgtatgct ggagtgcttt gcctatgaat tccaggaact taggctcatt
     4381 gagtatcaga tgatcagaag gtgccacgac aaccctggca tccgggttga tggcagcgat
     4441 cttatggctc acgtaggcaa cacagggagc agtgttacgg cgtgccggct cggccaggat
     4501 attggccgga ttcacatccg gtatctgctc taatatcagt cctacatagt cttcattggt
     4561 aacaatgaaa atgttatctt tggcaactat tttcaaaaac cgccggaagg tggtctgtat
     4621 cagggtctcc cccgtgttca ggatgtcgag gaattgcttt ggtttgctcg ccctgctttc
     4681 tggccagaac ctgctgccga tacccccggc catgatcgcc acataaacgt tatccatccc
     4741 tgcctatgta atttttgtac tccggttctg tttgttattc agtatattta aacacgcaca
     4801 ccggtagccc tgtgagagcg gaattgcaat gcaaagttgc ataaatatcc gttctgcaag
     4861 agtgaaattg accgttttag aattagtgac gtatgctaac cagtgaatta tcattgaaag
     4921 aagccctgca ggagtacttc ggctttaaca catttaaggg aaatcaggag gccatcatcc
     4981 aaagtatgct ggatggacgt gacacctttg tcatcatgcc caccggtggc ggcaaatcgc
     5041 tgtgctatca gctgcccgct gtcatgctgg agggcactgc catcatcatc tctccgctga
     5101 tcgccctgat gaagaaccag gtggacctgg tgcgcagcta cagcagtaat gacaacattg
     5161 cccatttcct gaactcgacc ctcagtcgca ccaccatcaa aaaggtgaag gccgacatta
     5221 cctcaggcaa gaccaagatg ctgtatgtag ctcccgaaac cctgaagaag gaagagaacc
     5281 tggaattctt ccggggcatc aaggtttctt tcatcgctgt ggacgaggcc cattgtatct
     5341 cagaatgggg ccatgacttc cgcccggagt accgccagat caagaccatg atcgggcaga
     5401 tcgatgcgaa gatcccgatc atcgccctta ccgcaactgc cacccctaag gtgcagagcg
     5461 atatcaaaaa gaacctcgac ctcgaagacc ccaacgtctt cctgtcgtcg ttcaaccgcc
     5521 ctaacctcta ctatgagatc cgccccaaac gcagccgcga gcaggccatc aagcagatcg
     5581 tcaagtacat acaggagcat cccaataaat cgggtattgt atattgtctg aaccgcaaga
     5641 gcacggaaga gatcgcagaa ctgctgaacg tgaatggcat caaggcggtg ccctaccacg
     5701 ccggtatgga agctgctacc agaacccagg tgcaggacca gttcctgatg gaagagatgc
     5761 atgtgatcgt ggccaccatc gccttcggaa tgggtatcga caagccggac gtgcgttttg
     5821 tcatccactt cgatattccc aaatccatcg agaactacta ccaggaaacg ggtcgtgcag
     5881 gtcgtgacgg actggaggga aaatgcatcg ccttctacag ctataaggat atcgagaagc
     5941 tggagaagtt catgcgtgat aaacctttgg ctgaacggga gatgggcgct cagctgctcg
     6001 ccgaaactgc tgcttacgca gagacttctg tctgccgccg ccgcttcctg ctgcactatt
     6061 tcggcgaagg atatgaccag gataactgcg ggcactgcga caattgtctc aacccgaaag
     6121 aaaagataga aggcaagacc ttcgtgcaaa ccgccctgaa agtggtgcag gctgtcaacg
     6181 agaattatgg cattccttat ctggtggacc tgctcatggg caagcgtacc acggagatcg
     6241 ggaattaccg gcacgaccag ctggaaatat tcggcagcgg caaagagaag gacgaccatt
     6301 tctggcattc ggtattgcgt cagtgtttgc tggaatcact ggtgtataaa gacattgaac
     6361 agtacggtct catcaggctt accgatgcag gacgaaaatt caacgacaag ccgaagaaca
     6421 tcaagctctc catcaaccat gattacgagg cagaagctgc cgatgtgcag gtggaaggaa
     6481 gtggtggaaa gagtgtgctc gaccaaaccc tgttcagtat gctggtgaag atccgcgaaa
     6541 gtgtggcgaa agaacaaaag ctgccacctt atgtcatctt cagcgaaagc tctttggaag
     6601 agatggctac tacctatccc tgcaatctgg aagagctgag caagataggc ggggtgagtg
     6661 ccggtaaggc gaacaaatac ggcgtcaaat tcgcagctgc tatcaagaag tatgtagagg
     6721 aaaatgagat cgagcgcccg ggcgacttca acaccatgaa gcaggtggcc aacaagagcg
     6781 tgcacaagat ccacatcatc cagaacatcg acaagaagct gccgctggat gagatcgccc
     6841 gcaaccgtgg gatgaagatg atcgagctga tcgatgagat cagcagcatc gtaaccagtg
     6901 gcaccaaact caacctcgac tattacctca atgacatcat agaagaggat ctgcaggatg
     6961 aggtcatgga tttcttccgc gagatggaaa gcggcgatct gaaagaagcg ttcaatgaac
     7021 tcaaggatga aggattgagc tacgaagagg tccagttact ccacatcaag ttcatgagtg
     7081 aggtggccaa ctaatcagca gactccttaa cttcgaaccc gatggcgtct aacgagacct
     7141 ttgagatcaa actgccccag ttcgaggggc cgttcgacct gctgctcttc ttcattgagc
     7201 gggatgagct ggatatctat gatattccca tctcccgcat caccaacgat tttctggatt
     7261 atatccacca gatgaaccag ctcaacatcg aggtggccag tgagttcatc ctggtggcag
     7321 ccaccctgat gcgcatcaaa gccaaaatgc tgctgcccag aaaagaactg gatgaatttg
     7381 gcaatgagat cgatccgcgg aaagaactcg tggatcgtct ggtggaatac aaacgctaca
     7441 aagacgtact ggaagaactg ggcaacctgg aagaccagcg ccgcttcaag atgcagcgtg
     7501 gcaatatcca ggccgaacat tctgtgctgg ccaaacagtt cagcaatgag atggagctga
     7561 gcgctgtcaa tctgttcacc ctgctgaagg tttttgaaaa ggtgatggaa cgctatcggc
     7621 atcagccgga agaggtagaa cacgtagtgt ttcagtacaa ctacaccatc gaagaagaag
     7681 cagacaatgt gatcagtttc tgcaagtcga aagaccgggt ggacttcgag gaattattct
     7741 ccacctgcgc agatcgtcac caggccatat tccgtttcct ctctgtgctc gacctgatcc
     7801 agctgcagcg aatcgatctg ctcattggcg aaggtttcaa tcagttctgg gtaagctaca
     7861 accagttccg cgaagatgac gcagcctgaa ccggacatac cggtttcaga tcaggccgaa
     7921 caactgaata aacagttcag ttatcgtaag gtcctctggg tcattgcact cagtctggtc
     7981 ctgtcaggct acctcatatt tgtctctttt gacgtagatg ccttccggct catctcctgg
     8041 actgccaaca gcctgcttct cctctttctg ggctatatcc tgctgggtat cagacacctg
     8101 gcatacatgt accgcatccg ccatctgacc aacaagcacc tgagctggcg acaaagtttc
     8161 gaggtcatca cactttggtt attcagctct gccgtgaccc cttctactgt aggtggtgct
     8221 gctgtggcgg tttatcttct taaaaaagaa aagctgagtg taggacaaag tgccaccacc
     8281 agtatgctca ctgtctttct ggatcaggca gtattcgcgg tcatcgcacc tctgttcacc
     8341 ctgcttgtag gcagtgaaga gatgttcgcc aaagacatgg gctgtcgcac ccagagtgaa
     8401 ctgcccatga tgcgtctctt ccacaatgta gagaccctgt attatgtggc ctacatcttt
     8461 tacctgctgg tggtgatcgg actggcctgg ggactttttg tcaatgccgg cgcattcaaa
     8521 tcctttctgg gcagattgtt ttctctcccg ttcctgaaac gctggaaagc agaggccctt
     8581 cgcaccggcg atgatattgt agccacctct cacatgctga aaggaaagaa cctgcgcttc
     8641 tggttgaaag ccgttggcag caccctgttg gcctggttat ctctctttct catcagcgta
     8701 tttattttca tggcattttt tgatcctgca acactggaat acctggtggt catggcccgg
     8761 caaacagtgg tctggatgat caccctgata cctgccacac ccggaggtgc cggagtagct
     8821 gagatcagtt tcagtgcttt gatgtgcgat atcaccagtc cggcactggt accaatcatg
     8881 gctttgctct ggcgcatgat ctccttttac ccttatctgc taatgggaat tttctttctt
     8941 cccagatgga tgaaacgggt atataacaac taaacatccg gttcatttat ctatgctgat
     9001 aaaaatcacc ctgtctccgg tcaggggtaa ttctaccttt gcatcatgga caacaagaat
     9061 ctccacccgg aaagcctgat gatgggcttt ggctataagc cggaactcag tgaaggggcc
     9121 gtgaaatgcc cgatctttca gacctccacc tttgttttca aaactgcaga agaaggcaaa
     9181 cgtttctttg agctggccta tggattgcag caggcagaag tgaatgaagt gcccggactg
     9241 atctacagcc gcctgaacaa ccccgacctg gagatcctgg agaaccgtct ttgtttatgg
     9301 gataaggctg aagactgcgc tgtgttcgct tccggtatgg gtgctatcac cacggtcatg
     9361 ctggaattcc tgcagcccgg agatgtgctg ttgtattcct ctccggttta tggcggaacc
     9421 gatcacagca tcaaccatgt gctgcccaag tggagtgtag gtcatgtgga attcaatgcc
     9481 tccatgcgca aggaagagat catccaaaaa gtggaacagg aacaaggtgg caaactggcg
     9541 atgatctatg tggaaacacc ggccaacccc accaatgccc tgatcgatct ggaaatgtgc
     9601 cgcgagatcg ccgatcattt ttccactgat gaacgaaagg tgcttgttgt ggtcgataat
     9661 acctacatgg gaccattgtg gagccacccg ctccagctcg gtgccgatct ggtggtgtac
     9721 agcgctacca aatacattgg aggtcacagt gacctcatag caggtgccgt gctgggcaac
     9781 agagagctga tgacccgggt tcgtggattg cgcacttttc tgggaaacat gtgcagtccc
     9841 tttaccggct ggctcatgat gcgcagtctg gaaaccctga aaccacgcat ggagacccag
     9901 gcagccaatg ccaggcaggt ggcagatttt ctgaaggatc atcctaaagt ggagaaactg
     9961 tattacctgg ggcatctgga agaaggcagt gaacagtacc gcatacgcga aaaacaatac
    10021 agctcatccg gtgccatgct gagcttcgat gtaaagggtg gagaggctga agctttccga
    10081 ttcctgaacg gactgaagct gatacgcctg gcggtttccc tgggaagtac cgaatcgctg
    10141 gcagaacatc ctgctacgat gacacatgcc gatgtatcac ctgcagataa actgaaacat
    10201 catatatctg aaagtctggt gcgtatatcg atcggtgtgg aacaccacga agatctgatc
    10261 gatgatatca gacaggcgct ggagaatgtg tgaggaaaga gaatacaggt agagatctga
    10321 ttgaccggtc agctcattct ctgatcagct aattactact tccgttccgc accttgcggc
    10381 gcagctcaaa gctctggccc agatagacct ttcgcacctg cggatcctct gccagttctt
    10441 ctgcactgcc ctgtttcagg atcttccctt caaacaacag ataagcacgg tctgtgatac
    10501 tcagggtctc ctgcacgttg tggtcagtga tgagcacacc gatctttttg aacttcagtg
    10561 tttccacgat gccctgtatg tcttctaccg caatggggtc gatgccggca aagggttcgt
    10621 cgagcaggat gaatttggga ttcacggcca gagccctggc tatttccgtg cgtcttcttt
    10681 caccgcctga cagtacatcg ccgttgcttt ttcgcacgtg tccaagaccg aattcatcta
    10741 tgagttcttc cagtttgtgt gcctgttcct tcctgctcag tttcgtcatc tgcaatacag
    10801 cggcaatatt atcttccacg ctcatcttcc ggaatacact ggcttcctgc ggcagatagc
    10861 ccactcccag ctgagcccgt ttgtacatgg gcagtttggt gatgggcttg ttgtccagat
    10921 agacttctcc tgcatcagcc ttgatgagcc ctacgatcat atagaaactg gtggtcttgc
    10981 cggcgccgtt ggggcccagc agcccgacga tctcaccctg ttccacctgc acactgacct
    11041 cgttcaccac ggtgcggcgt ttgtatttct tgaccagtcc ttctgagcgg agtatcatgc
    11101 gtgtacgcgg atgtctttga cgtttagttg cagattcgtc tggccgttga actcattctc
    11161 ttcgaggtaa taggccaggt cgaacggata gcccgaagat acatagggat aatgatctcc
    11221 ctgctggaag gcgatgccgc ttgcccggaa gcggtttctg tctcgcacat cgagtttgag
    11281 atgaccgttg cccaccacct tgctcctgcc gttgcaggtc agctcttccg tgatgaaaac
    11341 gggcggcagg ttttccggac cgaacggggc gaactgacga agtatcttgt aaaaaccggg
    11401 tgtgatgtct ttcaggttca gtaccgcgtc tatctcgatc tctggtatca gctgttcagg
    11461 tctgatggtt gacttgacca ccttctcaaa tctgtctttg aatgcctcta cattttccgg
    11521 cttcatggtg agtccggctg cgtacatatg tccgccgaac tgctccagga gatcgccaca
    11581 tgcactgatg gcctcataca catcatagcc ttttactgac cgtgccgatc cggcaaccac
    11641 accgttgctc tcggtcagca tgatggtggg tttgtaatag tggtccatga tgcgactggc
    11701 cacaatacca atgacccctt tatgccaggc aggattgaac agcacgttcg tgacccgttc
    11761 gggagtaagc ggatcattgt ccagcatctc cagggcttct gcagtgatgc tgcggtcaat
    11821 ttcttttctg tccatgttgt gcccctgcag atcgccggca tggttgcggg caaactgatc
    11881 agtactgctg atgagcatgc gcacagcctt ccgggcatca tccagccggc cggcagcatt
    11941 gatgcgtgga ccgagaccga acacgatctt gctgatgctc aggggtgggc gcacatcgct
    12001 gatctccatc agggctttca gacctttggt agggttggta tccatcttcc gcagtccata
    12061 aaacgcaagc actctgttct ctcctgtgat gggaacaatg tcacttgcaa tactcactgc
    12121 cacaagatcc aggaggtcaa gggcacggtc ttcgtccagt cctgcctcca gggtgatggc
    12181 ctgtgccagt ttgaaaccga tgccacaacc gctcaggtat ttatagggat agttgcaatc
    12241 cggctgatgc ggatcgagga tggccaccgc agcaggcagt tcatcgccgg gaaggtggtg
    12301 gtcgcagatg atcatgtcaa tgcccttgct attggcatag gctacctgtt ccactgcctt
    12361 gataccacaa tccagtgcaa tgatgagtgc acagttgttg tcttctgcat aatctatccc
    12421 ctgaaaggaa atgccatacc cttctttgta gcggtcgggt atgtagtaat ctatattagg
    12481 atagaagcgg ttgaggaagg agaacatcag cgccactgaa gtggtgccgt ccacatcata
    12541 gtcgccgtac accagaatgc gttcaccctg ttccgatgcc cgaaggatac ggttgacggc
    12601 ttcccgcatg cccttcatct caaagggatt gtgcagatcg ctcagttgcg gacgaaagaa
    12661 ttgtttggct ttatcgtatg tgtcgatacc cctgtccacc agcagcttgc acagtacggg
    12721 atggataccc agggcctcct gcaaaatatg cacggcaccg gcatccgact ctctgatcac
    12781 ccagcgttta tccattgttg tcaacttact ccactaccac cctggcagag ccggcaggct
    12841 ttcctgccga agtgcgtagt gtacaaacat agatgccggc ggggaggttc cggatatcta
    12901 ccagggcagt tgtacgaccg gatatttcgc ccgaccggca ggtcctgccc caaacgtctg
    12961 ctaaagtata aagaaaatac ccgccattgg tttgtatggt caggtagtct gttgcgggat
    13021 tgggatacag cccgaactgc acctcgtcca cctgtgcgat ggctgtcagg gtatcacaat
    13081 attcatccag catcagcatg gcattatata cgttcagccg gccaccactt acggtaatgg
    13141 tatccaggtc ttccagcgga tccacaccat ccagaataaa ttgcttcaat gccagcgcag
    13201 ccgtttccgg atcatgctta tattgatata gaaaattctc acaaccataa ctcagcatca
    13261 gcgccacagc acccgccaca tgcggagcag ccatggaggt acctgtctgg taattccagg
    13321 agttgcctac cacggtagag tatatcagcg aacccggtgc gcccagatca atagaaactg
    13381 aaccatatcc ggaggttgat ttattatcgg tcttgttggt gctgttcact gtaatgagaa
    13441 aatcactgct gcatgcggtg ggcatatcac caaccaggtc tatgttcaca ttggaattag
    13501 ccgttgctcc tgcactgaga atgcccaggg tacccaggct gtcatacatg ctgcaccaca
    13561 aaggaaagtc attcgggtct gcaaagtcga taccgaatga tgcattggtg gcaacaatga
    13621 aactcccctc tgttccgttg ctgtcatcat agcgtttgcg ttctgtatag acataaccgt
    13681 aacactccag cacattactc tctatcactg cacctgcacc gatggatagt ggcaggatct
    13741 gtacattcca gttgacaccg gtcacaccga gtgcattgtc accgcgggca cctgcaatgc
    13801 ctgacacatg ggtgccgtga ctgaattcag gcaagttatc agaattatcc aggctgttcc
    13861 agccatcaaa atcatcaata aagccgttgt cgtcgtcatc taatccgttt ccgggaatct
    13921 cagaataatt cttgcgaaac actaggtcat tgtggggcag gtagaaacca tcgtccacaa
    13981 tggctatcac aatggtatct ccttctgcgg tccagccacc ggtgctgaga tcccaggcca
    14041 gatctgcatc gatgtctgca tcatcggtac cacctgtaga accgtcgttg tacaaagccc
    14101 actgcaggtc aaaagagctg tcgtccggaa aacccctgat ctcgaggtag tggttgtact
    14161 gcagctgctt tacatcagga tgctgctgac aatatgcgaa tacctgttca tgagcttctt
    14221 ccggtatgga aatgacatac aggtgcagtg cctgcacaca gacataggcc aattgacaat
    14281 caaaacctgc ctgttgcagg tcggcgatca gctgttccgg ccgggcatcg ctgaaaagct
    14341 ccacgatcag ttgtcccggc acccggttgc tatcctgggc agatgtaacc accggcagga
    14401 gaaaccatat tatccagatc cacttcttca ttaacacgct taaaggtaag aagaatacag
    14461 gttgagctat aacgttcatc cctggtcata cttgttgcaa ggccatattt ccggcagaga
    14521 attgtaagac cagtttgcac ataatatcat tcatggcttt cattttactt ccctacagaa
    14581 tatatttacc ggacaaacac ccatgataca tttctttgaa aacctggacc cggtactgaa
    14641 gatcttctgg tacactgccc ttccggtaag tattattttt ctgattcaga ccatcatgac
    14701 ctttatgggg actgacggcg gtgacggcat gtcagcagat tttgacagcg atctggacgg
    14761 tgccgatgca ccatttcagc tcctgtcttt ccgaagcctc atccatttcc tccttggatt
    14821 cagctggtca ggtatttctc tgtttggtac catccacaat tccttgctcc tgatcgtggt
    14881 gtctgccctg atcggcggct tgttcgtact ggtcttcctc tggctgatgc agcaacttat
    14941 caaactggcg gagaataatt cctttaccta ccaggacaca ctggataaag ttggagaagc
    15001 ctatacacct attccgggac agaaagcggg gatcggaaaa gttctcatca gcatcaaggg
    15061 ttccacccat gaactagaag cgctgaccga cggcgaccgg atagaatcag gcaccccggt
    15121 tcgtgtactg cgactggaaa gcgacagaat tctcattgta gaaaaaatat aaaataaata
    15181 tctatggttt ctcctgttat catcgttgtt gtagcggcga tcgtattctt tgtgaccgtt
    15241 tccgtgctgg tatcaaggta caagcgctgc ccttcagaca agatcctggt catttatggc
    15301 cggacgggtg gtacatccgc caaatgtata catggtggtg gtgcatttat ctggcctgta
    15361 gtacaggatt atgcattcct ggacctgaag cccttgtcta ttgaagccaa cctcaccaac
    15421 gccctgagcc ggcagaatat acgggtcgat gtgccctgtc ggttcacgat cgccatctct
    15481 actgaggctg acaatatgaa cactgctgca gaacgtcttc tgggactttc ttcagaacag
    15541 atccaggaac tggctaaaga catcctgttc ggtcagctgc gtctggtcat tgctaccatg
    15601 acgatcgaag agatcaactc agaccgcgat aaattcctgg agaatatttc gaagaatgtg
    15661 gattcagagc tgaagaagat cggcctgaaa ctcatcaacg tgaacgtaac agatatcaaa
    15721 gatgaatcgg gttacattga agcacttgga aaggaagcag ccgcaaaagc catcaacgat
    15781 gccaagatca gtgtggccga acaggaaaag atcggggaga ccgggaaggc tcttgcagac
    15841 agggagaaag atgtacagat agcagaaact catcgagacc gtgatgtgaa gatcgccata
    15901 acacagaaag accgggaggt gagtattgcc tctgcgcaca aagatgaggc tatcggaaaa
    15961 gcggaagcag acagagatac ccgtatcaaa acatctgaag cgaatgcatt tgcaattgaa
    16021 ggagagaata ccgccaagat ctccattgcc aattcagatg caaacaggcg ggaaaaggag
    16081 gctgaggcct tacgggtggc catcacagct gagaaagttc agcaggccaa agccctggaa
    16141 cttgcctacc aggcagagca aaaagcggag acagcccgaa gtgagcggga acgttctact
    16201 caggtggcta acgtggtgat acctgctgag atctccaagc aaaaagcgat cattgaggct
    16261 caggccattg ccgaagctgt gcgtgaaaat gccaagggcg aagctgatgc catctatgcg
    16321 aaaatggagg ccgaggcaaa aggtctttat gagatcctga ccaaacaggc cgaaggttac
    16381 cgtgaagtag tggctgctgc tggcggtgat cctacaaaag cattccattt attgctgata
    16441 gagaaattgc ccgagctggt taagacacag gtggaagcca tcaagaatat caagatcgac
    16501 aagatcactg tctgggattc tggtaatgat aaagaaagtg gtcagggttc cactgccaat
//
LOCUS       DAOMAX010000029        16036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_399_length_16036_cov_4.098742, whole genome shotgun sequence.
ACCESSION   DAOMAX010000029 DAOMAX010000000
VERSION     DAOMAX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16036
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_16036_cov_4.098742"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PK742_02960"
     CDS             complement(<1..886)
                     /locus_tag="PK742_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75578.1"
                     /translation="MKKGLLSLISSVAVLLTNAQTDVYLQIHHMLGDAPFAYEATAST
                     NLSEDFQVTRLEYYLSEITLIHDGGQETTIPDLWLLVDAGETVNAFLGSHDITTLESV
                     RFGVGVDQEHNHLDPSAYPGDHPLAPQLPSMHWGWASGYRFVAMEGWAGEGFAQNFQI
                     HALGDINYRTTEIATSGAILDGSLVVAIEGDYAQAIRDISVAAGVISHGETEQSAELL
                     WNFHDFVFKEGEASVVSSLDNPGAASIVVAPNPAGSIVQIRIPGQMTSGNNIALTDYS
                     GRQIMQVPAESQVTLDVSD"
     gene            complement(949..2166)
                     /locus_tag="PK742_02965"
     CDS             complement(949..2166)
                     /locus_tag="PK742_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75579.1"
                     /translation="MKTICTSLLLTVLTAWSVNSIAQIVGTNAFIQGDYVEVGINDHG
                     TYIADEAAPAGYHDAGGFTGFGMVADQGKDGWDVGSPMFCGDYFEPGSPEEGFAIKWD
                     GASYYNTFYVEDIDGSVLSYNATPVGANVVWRGSVPAAGLQVTQVTTVPIDRSTVLTT
                     VKIKNTGDLPVLNVYYSRNADPDNDFESGATFVTENIVVQNHPVDPWALVSATGTSAG
                     CYLAMASKQTNSIASYGAFSTAGMDPAAAYNGISPYSTSGSTTGDMAIQMSFRIPTIL
                     AGETKVINFIYGTNQSDLEDLVMFTTDEVARGMEESINGYASFQPDFRVSPNPSSGLL
                     NLLAFGFDEFASLQLAVMDLTGRMVFQTNLSNDQGRALYSNELPSDMVNGTYLAVLYV
                     NGVPYTEPFVLQR"
     assembly_gap    2470..2569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2765..3133)
                     /gene="ssrA"
                     /locus_tag="PK742_02970"
     tmRNA           complement(2765..3133)
                     /gene="ssrA"
                     /locus_tag="PK742_02970"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3201..4895
                     /locus_tag="PK742_02975"
     CDS             3201..4895
                     /locus_tag="PK742_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQU75580.1"
                     /translation="MQEENNQALTDLFPPILPSLDDWPIVKISKDRQEIVRAVAERTS
                     AQILAKNPGEKSIQDVLAKAVYLEKARISSKPWSIDPKDDKAFWDGIKKELVRLEAFA
                     DNDITEEAAVLERIVERYAREIAGHFDKNTYRFANRFVPFAFNRLLNSTRSREFIRLF
                     RNKRNLLKRIHLVGETEQIRSLARKGTVIAVPTHISNIDSITIGWAISAIGLPAFLYG
                     AGLNLFSVRFLAYFMKRLGAYKVDRRKKNSIYLETLKTYSTEVIERGGHSLFFPGGTR
                     SRSGELESNLKLGLLGTALEAQRQHIIQADSNEFRRIYVVPVTLNYSFVIEAPQLIDE
                     HLRQVGKERYFIQADRYSSSYKLLKFIYRFFSSSADFTIAFGQAMDIFGNKVDEDGNS
                     IDEFGNKIDIRDYFRSNGVLVEDVQRDNEYSRMLSEKIVQAYRVNNVAMPAHIVSFAA
                     FHIFQQMHTTSDLYSVLRMPAEFRRIPYQKLLQSVKNLQDELVRMSEKGKIRIGALVE
                     AEPEELLQYGLKSLGVYHAKKPLRKEKKTGDIVCQDMKTLLFYHNRLTGYGLEKHV"
     gene            4888..5931
                     /locus_tag="PK742_02980"
     CDS             4888..5931
                     /locus_tag="PK742_02980"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQU75581.1"
                     /translation="MSELPYVGVIGAGSFGTVIANLLAENRQVLLYTRRSEQAAVMQE
                     SRMNAGQQLHARIYPTTDLADMASRCNLLFPLVPSQNFKEMIRTLAPHLRPDHLMIHG
                     TKGLAIRKKGRKMLRQEGVLPREYVLTMSELIMRESVVVRVGCLAGPNLSKEMAKGLP
                     AAATIASRFDEVIREGQDALKSNRFHVYGGHDMFGIELAGVLKNILAIGSGVVSGLGM
                     GENSRAMLITKGLSEIIHLAKAMGADVRSFLGLAGIGDIIATCTSPTSRNYTVGYRLA
                     QGETLEAILASMEEPAEGVNTTRIANGLVRYYGLTSPIITTLYKGLFEGLSLPAAIEY
                     LLNYRFSMDVDFI"
     gene            complement(5932..6882)
                     /locus_tag="PK742_02985"
     CDS             complement(5932..6882)
                     /locus_tag="PK742_02985"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQU75582.1"
                     /translation="MSSLSVKHLISIRSLQTDDIQLIFSTADHFKEVLNRPIKKVPSL
                     RDITVANIFFENSTRTRISFELAEKRLSADVVNFSSSGSSVKKGETLLDTVNNILAMK
                     VDMVVMRHQSPGAPVFLSKHIDANIINAGDGTHEHPTQALLDAYSIREQIGKRNGKVP
                     ENFNGLKVAILGDILHSRVALSNIYCLQKLGAEVMVCGPPTLIPHDIASLGVQVTYNV
                     REALNWCDVANILRIQLERQDIKFFPSLREYALYFGVNRKLLEEVGKDIIIMHPGPIN
                     RGVEITSDVADSGQSIILQQVENGVATRMAALYLLAGKKE"
     gene            complement(6879..7385)
                     /gene="pyrR"
                     /locus_tag="PK742_02990"
     CDS             complement(6879..7385)
                     /gene="pyrR"
                     /locus_tag="PK742_02990"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQU75583.1"
                     /translation="MERLCHQLIENHTDFSGSCLIGLQPRGIFLADRIRKQLHELLPG
                     ISIPYGVLDPTFHRDDFRRNEKTLVPAPTHIPFSIENKRVVLVDDVFYTGRTVRSGLD
                     ALLDFGRPASIELLVLIDRRFSRHVPISPDYVGKRVDAVMEEKVRVDWQEVAGKDEVW
                     IIPYKATA"
     gene            7269..7502
                     /locus_tag="PK742_02995"
     CDS             7269..7502
                     /locus_tag="PK742_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75584.1"
                     /translation="MQLFTYSVGQKDASGLQPDQTGTRKISVIFYQLMAKPLYSKGEL
                     GAVKNDPALHQDAKLRNFSEMPEITTGDHLIAC"
     gene            complement(7520..8458)
                     /locus_tag="PK742_03000"
     CDS             complement(7520..8458)
                     /locus_tag="PK742_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75585.1"
                     /translation="MATKGRTIERVVYITAIAGLLGLNAYLFTNIRSDKAVQADTEQR
                     MSEMDSLNVVLDLKYNNIVDELTRQKGVSSVQDAYIDSVLADLAERKSDINGLLKSRD
                     VMTMEKGEAERLLKAASAKIDELETDKQEYITQIAEMTAAYEKLSNDFEIVYSNYETE
                     MRKSKRLATERDSVAYLGSAIMAKNISMVGLKSKGEGKEKEATKAKSADKIKVCFDLV
                     RNGLSAGKQQTFYVKIIDPNGVTVQSTESMYKSIPGINGSKKGYTASLTVDYKGDNSD
                     SYCVYWEQNYEFISGTYTAEVYHQGQLVGSNTFDFK"
     gene            complement(8559..9713)
                     /locus_tag="PK742_03005"
     CDS             complement(8559..9713)
                     /locus_tag="PK742_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HQU75586.1"
                     /translation="MQLVATTLQGLEQVLAEEIRSLGAEDIRIGKRAVEFTGDQYTLY
                     AANVYCTTALRILKNIKVFQARTPEELYTLSKEVNWEEFLHADQTFAIDFTVHSPYFT
                     HSQYAALKLKDAIADRFRERLGRRPSVDTKAPHIYIHLRIDQDQVFLSLNSSGESLHR
                     RGYRGQQHKAPLSEVLAAGMIRLTGWDGKTPFYDPMCGSGTLPIEAAMLADHLAPGLL
                     GRKYAFQHWPDYHEKLFRQILLDAQKQVLTTEKSMIFASDTDHKSYSLSKENAQRAKL
                     DHRIHFFLSSFEKASPPPVAGTLIMNPPYGERLPHADLVSFYKAIGDVMKQRYQGWQV
                     WILTANLEAAKFIGLRPSRKIQLFNGPMECRFFRYDMYAGSKKTLSQDTP"
     gene            complement(9790..10296)
                     /locus_tag="PK742_03010"
     CDS             complement(9790..10296)
                     /locus_tag="PK742_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75587.1"
                     /translation="MKTTMKLMLTLLFAGALSLGSQAQVVMKDFMSANHMGKVENSLN
                     NPGKPLYWKLEYKSTEGARIYYTLTFYKDAAMSQPMVSFPSLMRNLEWTYYLDVSMTK
                     DDATKVFAMIFKKDLRWSRVKYTPHQDCGWQDPTKWDRYNQVDDFQKLLDNTMMQLDK
                     NVKLSCYM"
     gene            complement(10319..11704)
                     /locus_tag="PK742_03015"
     CDS             complement(10319..11704)
                     /locus_tag="PK742_03015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HQU75588.1"
                     /translation="MSTKQVFALVLAGGMLLAAGCKGKESSDVANPLYNSVSQNSSDQ
                     SGTFLVDWWKQLFSFVASEKLSPPDASRMYAYIGTTMYEAQVCGAPDYMSLEGQLNEL
                     KDLPRPNKDEVYDWTTVTIESVYYVQDEMLGRYLPAGVAAINKLHEKQISERAATVPE
                     DVMMRSKEYGRALADAIIAWSETDGYERTRYEQYKAPSREGHPEYWEPTDFNQTALEP
                     FWGTHRTFAVPNNKMCDVDMKFAYDTDPNSDFYKEAMEVYTVDKNLTEDQRIVAQYWA
                     DDPGETATPPGHWINMSCNFVLAENWNLEKAAEMYALVAVSMQDACISLWHTKYRVNL
                     VRPKTYIEENIEPGWEPYVETPPFPGYTSGHSGFSGAAAEVMTALIGDNKPFTDSTHV
                     KIGLEPKTFNSFRDAAQEAAYSRMYGGIHYTCEIMDGLEQGKCAGDYVLNKIVTDTHR
                     KGMKSEEVAAK"
     gene            11897..15493
                     /locus_tag="PK742_03020"
     CDS             11897..15493
                     /locus_tag="PK742_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQU75589.1"
                     /translation="MKYVVYPVIATMLLTAGCSKKQSFQDRMNEWKFTENIDQAKVVP
                     LDGNTGKMFDILTGATTGVNFNNEIKETFELNYYRYGYSYNGAGVGVGDVNNDGLPDL
                     YFAGNVVPDRLFINKGNLQFEDITESCGINGKGGWSTGVTFVDINEDGFQDIYVCRAR
                     FDDADMRRNLLYVNNGDNTFTEKAAEYGIDDDSYSTHANFFDADNDGDLDLYVVTHPT
                     DFKDKNKQKNFQKIEQGVNQSNRFYRNEGNGKFVECHQEVGINNHGFGLSVTAGDLNN
                     DGWQDIFVGNDYIMHDYTYINQGNGTFKESSRELLTKTAYYGMGTDVADYNNDGLLDM
                     MTVDMEIEGNYGTKTFMQSNKQTFLRTLVNAGYLQQYGRNNLQLNNGMGKFSEVANFA
                     GVSTTGWSWSPLFADYDNDGNKDLYVTNGFMKDSHMDVMEVYIKLTRANRLSDSTDYY
                     ELRKMLPENSVLQFPNAMFHNTGDLTFDDVREDWGLYHPSMTYGGAYADLDGDGDIDL
                     ICNNANYPAFIIRNNEDKMMGNNYLRIDLKGNKSNPEGLGAKVYVTNGNTTQYVQLET
                     VKGYMSCSESVAHFGMGKNTTADKVVVRWLDGKENVMTNVPVNQLLVIDHKDAVYNKV
                     SEVPGQQPFFVDATNELKIDYVHKENEFDDFDREFLIPHELSNMGPGIAVADVNGDGL
                     DDFFVGGARMSAGVLYVQNTNGTFSRSQGYNPGADEIECEDMGAIFFDADGDGDQDLY
                     VVSGGSDFPKNDPHLQDRLYMNNGGNFALNKEALPAMMTSGSSVAANDFDKDGDMDLL
                     IGGRLVPGDYPVAASTYILRNNGGKFSDVTAEIAPDLAKAGLVSAVLWTDFNNDNQTD
                     IIVTGEWMPIMVFQNKGGKFQNITKNAGLDKSTGWWNSLTAGDFDNDGDIDYIAGNFG
                     LNCRYKPKEKPLELFYDDYDNDGRHDIIMCYYQKDKLYPIKTRERMIEQIPSIAETFP
                     NWDSYGRAEVWDFYGKERMEKSQGHYTAESFNTSYIENLGNGKFAIRKLNNVMQISTV
                     FGMETMDLNGDGFLDLIAHGNWYEMEIETNIQDAGIGNVLLGNGDGTFTPVHSRFSGF
                     YSPGNAKGLAKLTVGKDKTPVILASNSNDKMYAFKYIGKHRTVTLQADDSYAIVTMND
                     GKQRRMEFYTGASYLSQSDKFMLVTPEMKSVIVYNNKGESRELTANGWVAVK"
     gene            15517..>16036
                     /locus_tag="PK742_03025"
     CDS             15517..>16036
                     /locus_tag="PK742_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQU75590.1"
                     /translation="MKNPIAKISLLSLSAGLMLISSCKLFSGGDSDTRGSVIIDKARE
                     VTLDGNSGKRFDILTGKSTGIDFVNDLTENYEVNWWRYSYIYNGGGVCIGDVNKDGLP
                     DLFFTGNLVPNKLYINKGNMQFEDVTAQSGIVKEEWEFSYGATMVDFDGDNDLDIYIC
                     NSRWDKPEKRKNR"
BASE COUNT         4330 a         3766 c         3753 g         4087 t
ORIGIN      
        1 gatcactcac atccagtgtg acctgacttt ctgcaggtac ctgcatgatc tgtctgccac
       61 tgtagtcggt cagagcaata ttgtttcctg atgtcatctg tccgggtatg cggatctgca
      121 cgatggaacc ggcagggttt ggagctacca cgatagaggc cgcaccaggg ttatccaaac
      181 tactgacaac tgaggcttct ccttctttaa aaacaaaatc atggaaattc cacagtaact
      241 cagcagactg ttctgtttca ccatggctga tcacaccggc agccacagaa atatcgcgaa
      301 tggcttgtgc atagtctcct tcaattgcaa caaccagaga accgtccagg atcgcaccgg
      361 aggtagcaat ttcagtagtc cgatagttga tatctcccag agcatgtatt tgaaagtttt
      421 gagcaaaacc ttcacctgcc cacccttcca tagccacaaa cctgtatcct gatgcccaac
      481 cccaatgcat acttggcagt tgaggtgcca ggggatggtc gccgggatag gcagaaggat
      541 ccagatgatt atgctcctga tctacaccta caccgaagcg tacagattcc agtgtagtaa
      601 tgtcgtgaga gccaagaaag gcatttaccg tttcacctgc atccaccaat aaccacagat
      661 caggaatagt agtctcctgc cctccatcgt gaatgagtgt gatctcagac aaataatatt
      721 ccagtctggt tacctgaaag tcttcagaca gattggtaga agccgttgct tcataagcaa
      781 agggagcatc gcccaacata tggtggatct gcagataaac atccgtctgt gcatttgtca
      841 gtaagacagc aacagatgaa atcagtgata gtaaaccttt cttcatagtg tacaaaaata
      901 aaaaaaggcc ggagatccgg ccttataatc attgaattct aagtgaaatt atcgctgcag
      961 tacgaagggc tctgtataag gtaccccatt cacatacagt acagccagat aggttccatt
     1021 caccatatct gacggaagct cattgctgta taaagcacga ccctggtcgt tagagagatt
     1081 agtctggaag accattcttc cggtcaggtc cataacagcc agctgcagtg aagcaaattc
     1141 atcaaatccg aaagccagca gattcagtaa accggagctt gggttaggag acaccctgaa
     1201 atcaggttga aaactggcat agccattaat ggattcttcc atacctcgtg ccacctcatc
     1261 agtggtgaac atgacgaggt cttccaggtc agactgattg gtaccataga tgaagttgat
     1321 caccttggtt tctcctgcca gtatggttgg gatgcggaag gacatttgta tggccatatc
     1381 acctgtagtg gacccgctgg tactgtaagg agaaatgcca ttataagcag ctgcaggatc
     1441 cattccggca gtagagaagg cgccataact ggcaatagaa ttggtctgct tggaagccat
     1501 ggcaagatag catcctgcag atgtaccggt agctgagacc aaagcccagg ggtcaacagg
     1561 atggttctgt accacgatat tctcagtaac aaaagtggct cctgattcaa aatcattatc
     1621 agggtctgca tttcgtgaat aatacacatt gagtacaggc agatctcctg tattcttgat
     1681 cttcacagtg gtgagtacgg tacttctgtc tataggaaca gtggtgacct gagttacctg
     1741 aagaccggcg gcgggcacac tgcctctcca gacaacattg gcacctaccg gggtggcatt
     1801 gtagctcaat acagagccat caatgtcttc tacatagaag gtattgtagt aactggctcc
     1861 gtcccacttg atagcaaaac cctcttcagg tgaaccaggc tcaaagtaat cgccacagaa
     1921 cataggtgaa cctacatccc agccatcttt accctgatct gctaccatgc caaagccggt
     1981 aaacccacct gcatcatggt agcctgccgg tgctgcttca tcagcgatat aggtaccatg
     2041 atcattgata cccacttcga cataatctcc ctgaataaag gcatttgttc cgacgatctg
     2101 tgcgatactg tttactgacc aggcggtcag gacagtgagg agtaaactcg tacaaatagt
     2161 tttcataacg gtgtgtattt agttacttta aattacacat tctctatcag ttatccggat
     2221 aaaatcatgc agatgacata tttatgacaa tcgcatacac aaatacctct cgatgatgat
     2281 ccaacaatag gctttcggcc aaacctttca tggaaggtgc atcgtattat tacctatgtg
     2341 aatgccggta aatctgcatt accattgtca ggcggatcaa actgccatgt tttccttaca
     2401 tcgcctggtg cgctatgggt ttctttttga aaatgcctcg gcgggattgg ctccgcccat
     2461 ccctcgggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgggtttctt
     2581 tttgaaaatg cctcggcggg attggctccg cccatccctc gggctcccgt gtggcaaatc
     2641 ttttgacctt acatcgcctg gtggcgatgt gggtttttca tgatacaaat gaccttgcaa
     2701 gcgagcttgc ggtcatttgt atcatgaaaa acctccaccc tgcgggtgga ggtcaacggt
     2761 ttacaagtgg agctggcggg attcgaaccc gcgtccagac aaagcatttt caggctttct
     2821 ccatgctcag caataccttg tttgtaggga taaccagggt ggtattgctt acctgaggat
     2881 atccttagtt ccttgatctc atgccacagc ggaacaatgc agcaccagcc ctgattagat
     2941 gaagcttgct gcagttgtat caaggcacac ttctgcagaa gctaaggcta ccttattcct
     3001 tgaattaggc tgccatggcg tagttatact cgccgtataa aaagagtgtc ggtcattagt
     3061 ttgaacgagc caaaaaccaa agctcggcat gcttacctgt caatgaacga tgctgtcaat
     3121 tccggtcagc cccggaagtt caaagataac aaacgccggg gctgctttaa agttccatca
     3181 agccggtata tttgtcaggc atgcaggaag agaataacca ggcattaact gacttgttcc
     3241 cgcctatcct accatcattg gatgactggc ctatagtcaa gatcagtaag gaccggcaag
     3301 agatcgtaag agcggtggcc gaaaggacct cagcccagat cctggccaaa aaccccggcg
     3361 aaaaaagcat acaggatgtg ctggctaagg ccgtgtatct ggaaaaagcc cggatcagca
     3421 gtaaaccatg gtctattgac ccaaaagacg acaaggcatt ctgggatggt ataaagaaag
     3481 aactggtaag gctggaagca tttgctgaca atgacatcac tgaagaagca gctgttttag
     3541 agaggatcgt agaaagatat gccagagaaa ttgccgggca ttttgacaag aatacctatc
     3601 ggtttgccaa tcgttttgtt ccttttgcat ttaaccgcct gctgaattct accagaagca
     3661 gagaattcat cagacttttc aggaacaagc ggaacctgct gaagcgcatt catctggttg
     3721 gtgagaccga gcagatcagg tcgcttgcca ggaaaggaac ggtcatagct gttcctaccc
     3781 atatatcgaa tattgattcg atcaccatcg gttgggctat cagtgcaatc ggccttcctg
     3841 ctttcttata cggtgccggg ctgaatcttt tcagtgtgcg ctttctggct tatttcatga
     3901 aacgcctggg tgcctataag gtggacaggc gcaaaaagaa cagcatttat ctggaaaccc
     3961 tcaagaccta cagtaccgag gtcattgaac gcggaggaca ttccctgttc tttccgggtg
     4021 gtacccgttc gcgtagtggc gaactggaat caaatctcaa actcgggttg ctgggaactg
     4081 ccctggaagc acagcgtcag catatcattc aggctgatag caacgaattt cgcaggatct
     4141 atgtagttcc ggtaaccctt aactacagct ttgtgataga agcacctcag ctcattgatg
     4201 aacatctgcg ccaggtagga aaagagcgct atttcatcca ggcagatcgc tattcctctt
     4261 catataaatt attaaagttc atatacagat ttttcagctc aagtgcagat tttacaattg
     4321 cattcgggca ggcgatggat atatttggaa acaaggttga cgaagatggc aatagtatag
     4381 atgaattcgg aaacaagatc gatatacgag actatttccg atcgaacggt gtgctggttg
     4441 aggatgtaca aagagacaat gaatacagca ggatgctgag tgaaaagatc gtacaggcat
     4501 acagggtgaa taatgttgcc atgccagcgc acattgtttc atttgctgct ttccatattt
     4561 tccagcagat gcacaccacc agcgatctct attcagtgct gcgtatgccc gcagaattca
     4621 gacgaatacc gtatcaaaag ttactgcaat ctgtaaagaa cctgcaggat gaactggttc
     4681 ggatgtcaga aaaagggaag atcagaatag gtgccctggt agaagctgag cctgaagaac
     4741 tgctgcaata cggactgaaa tctcttgggg tatatcacgc caagaagcca cttagaaaag
     4801 aaaaaaagac aggcgatatt gtttgtcagg atatgaaaac acttctcttt taccataaca
     4861 ggttaacagg ctacggcctg gaaaaacatg tctgaactgc catacgttgg agttataggt
     4921 gccggtagtt tcggtacagt gatcgccaat ctgctggctg agaacaggca ggtactgttg
     4981 tacaccaggc gatctgagca agctgctgtc atgcaggaat cgaggatgaa cgcaggtcag
     5041 caattacatg cacgtattta cccgacaaca gacctggcag atatggcttc caggtgcaac
     5101 ctgttgtttc cgttggttcc gtcgcagaat ttcaaagaga tgatcagaac gctggcgcca
     5161 catctcagac ctgatcacct gatgatacat ggaaccaaag gactggccat tagaaaaaaa
     5221 ggcagaaaga tgctccgtca ggaaggagtt cttcccagag aatatgtatt gaccatgagt
     5281 gaactgatca tgcgggagag tgtcgtagtc agggtcgggt gcctggcagg tccaaacctt
     5341 agtaaagaaa tggccaaagg attaccggct gctgcaacca tcgccagccg gtttgatgag
     5401 gtcatacggg aagggcagga cgctctgaaa agcaatcgtt tccacgtata tggtggccat
     5461 gatatgtttg gcattgaact ggcaggtgtt cttaagaaca ttctggccat aggcagcggg
     5521 gtggtgagcg gacttgggat gggagaaaac agccgtgcca tgctcattac caaagggctc
     5581 tcagagatca ttcatctggc aaaggctatg ggtgcagatg ttcgttcatt tctggggctg
     5641 gcaggaatag gcgatatcat tgccacctgt acttccccaa ccagccgaaa ctataccgtt
     5701 ggttaccggc ttgcgcaagg agaaacactt gaagccatac tggcctccat ggaagaacct
     5761 gcagaaggtg tcaataccac ccgcattgct aatgggctgg tcaggtatta tggtctgacc
     5821 agtccgatca ttaccacctt gtacaaagga ttgtttgaag gtttaagcct tccggcagcc
     5881 atagagtacc tgctgaatta ccggttcagt atggatgtag attttatctg atcactcttt
     5941 tttgcctgcc agcagataca gggccgccat tcttgtagcc accccgtttt ccacctgttg
     6001 cagaatgatg ctctgacccg aatcagctac atcgctggtg atctccaccc ctctgttgat
     6061 cggcccgggg tgcatgatga tgatatcttt tcctacttct tccagcaatt tcctgttcac
     6121 accgaagtac agggcatact cccgcagaga gggaaagaac ttgatgtcct gacgctccag
     6181 ttgaatgcgc agtatgttgg ccacatcgca ccagttcagt gcttcccgta cattgtaagt
     6241 tacctgtaca cctaatgacg caatgtcatg aggaatgagt gtaggaggcc cgcagaccat
     6301 gacctcagca cccagttttt gcaggcaata aatattggaa agagccactc tgctgtgcag
     6361 aatatctccc agaatagcca ctttaagacc attgaagttc tcaggcacct ttccattccg
     6421 tttaccaatc tgctctctga tggaataagc atccagtaag gcctgggtag gatgttcgtg
     6481 cgtaccatcg ccggcattga tgatattggc atctatgtgt ttgctcagaa agaccggagc
     6541 acccggactc tggtgccgca tcaccaccat gtcaaccttc atcgccagga tgttgttgac
     6601 cgtatcgagc agggtctccc ctttctttac cgaagaaccg gaactactga agttgaccac
     6661 atcggcactc agtctttttt ctgccagctc aaatgatatt ctggtacggg tagagttttc
     6721 aaagaatata ttggccactg tgatatcacg caaagacggc accttcttga ttggccggtt
     6781 aagtacttcc ttaaagtgat ctgcagtgga aaaaatgagt tgaatgtcgt ctgtctgaag
     6841 cgaccggatg gagatcagat gtttgacact cagactactc atgctgttgc tttatagggt
     6901 atgatccaga cttcatcctt accggctacc tcctgccagt caacacgtac tttttcttcc
     6961 attacagcat ccaccctttt acctacataa tcgggtgata taggcacatg cctgctgaat
     7021 cttctgtcta tcagcaccaa cagttcaata gaagctggtc tgccaaaatc aagcaatgca
     7081 tccaggcctg aacgaaccgt tctgccagta tagaagacat catctaccag cactactctt
     7141 ttattttcga tagaaaaagg gatgtgcgta ggggcgggca ccagggtctt ttcatttctc
     7201 cggaaatcat cgcggtggaa ggttggatcc agcactccgt acggtatgct gatgccggga
     7261 agcaattcat gcaactgttt acgtattctg tcggccaaaa agatgcctct gggctgcaac
     7321 ccgatcagac aggaaccaga aaaatcagtg tgattttcta tcaactgatg gcaaagccgc
     7381 tctatagtaa gggagaactt ggtgctgtca agaatgaccc ggctttgcat caagatgcaa
     7441 aattgcgcaa tttttctgag atgcctgaaa taacaacagg cgaccatctg atcgcctgtt
     7501 gaaatatgca gctaaagggc tatttgaaat caaaagtgtt gcttcctacc agttgtccct
     7561 gatgatatac ttcagccgtg taggtacctg aaatgaattc gtagttctgc tcccagtaaa
     7621 cacaataaga gtcagagtta tctcctttgt aatcaacggt taggcttgcg gtatatcctt
     7681 ttttactgcc gttgataccc ggaatggatt tatacatact ttctgtgctt tgtacggtaa
     7741 ctccgttggg gtctatgatc tttacataaa atgtttgctg ttttccggca ctcaatccat
     7801 tgcgaacaag atcaaaacaa accttgatct tatctgcaga ttttgctttt gtagcttcct
     7861 tttcctttcc ttcccccttg gatttcagac caaccatgga gatgtttttt gccatgatgg
     7921 cacttcccag ataagcgact gagtctcgct ctgttgcaag ccgcttactt tttcgcattt
     7981 ctgtttcata gtttgaatac acgatttcaa agtcattgga tagtttttca taggctgcgg
     8041 tcatttccgc tatttgggtg atgtattcct gcttatctgt ttccagctcg tcgatcttgg
     8101 cgctggcagc cttgagcagc ctttcggctt ctcctttttc catggtcatg acatctctgc
     8161 ttttcagcag gccgtttatg tcactcttcc tttctgcaag atcggccaat acagaatcaa
     8221 tataggcgtc ctgcacagag ctgactcctt tttgccgtgt cagttcatcg acaatattgt
     8281 tgtatttaag atccagaacg acattgagtg agtccatttc actcatgcgt tgttcggtat
     8341 cagcctggac ggctttgtcg ctccgaatat tggtgaacag gtaagcatta aggcccagaa
     8401 gccccgctat tgcagttatg taaacgaccc gttcgatggt tctgcctttc gttgccataa
     8461 tcaagggttt tagttcctca atataacaaa gagcttcaaa acctgcacaa aaacagctca
     8521 atttgtgtaa acaggcaaga tcacaattcc cacctcattc agggggtgtc ttgtgacagg
     8581 gttttcttac ttccggcata catatcgtag cggaagaacc ggcattccat tggcccattg
     8641 aaaagctgaa tctttctgga tggccgtaaa ccaatgaatt ttgccgcttc caggttagca
     8701 gtcaggatcc aaacctgcca accctgatac cgttgtttca tgacatcacc tattgctttg
     8761 tagaaagaaa ccagatcagc atggggcaat cgttcaccat agggtgggtt catgatgagg
     8821 gtaccggcaa ccggaggcgg gctggccttt tcaaatgaag acaaaaagaa atgaatacga
     8881 tggtccagct tagccctttg cgcattttct ttggacagag aatagctttt atggtcagta
     8941 tctgacgcaa aaatcatgga cttttctgtt gtcagcacct gtttctgtgc atccagcaag
     9001 atctgtctga ataatttttc atggtaatca ggccagtgtt ggaaagcata ttttctaccc
     9061 aacagtcctg gagccaggtg gtctgccagc attgcagctt caattgggag ggtgcctgag
     9121 ccgcacatag gatcataaaa cggggttttt ccatcccatc cggtaagtct gatcattccg
     9181 gcagccagta cctcactgag tggtgcttta tgctgttgcc cgcggtagcc ccggcggtgg
     9241 agagactctc cgctgctgtt gagcgaaaga aacacctgat cctgatcgat acgcaggtga
     9301 atataaatat gcggtgcctt ggtatctaca gaaggccttc ttcccagacg ttcgcgaaac
     9361 ctgtcggcaa tggcatcttt cagtttcaga gcagcatact ggctatgtgt gaaataagga
     9421 gagtgaacag taaagtcaat ggcaaacgtc tgatcagcgt gcaggaattc ttcccagttc
     9481 acctcttttg ataaggtgta cagctcttcc ggggtacggg cctgaaatac tttgatgttt
     9541 ttaagtatgc gcaaagctgt ggtacaataa acattggcag catacaatgt atattgatcg
     9601 ccggtaaact caacagcccg tttcccgatg cggatatctt cagcccccaa agatctgatc
     9661 tcctctgcca gaacctgttc cagaccctgc agggtagtag ctaccagttg catgcagcaa
     9721 aggtagaaga agcgcagaca aaaaaggccc cggtaaaacc ggagccctta aaatcattca
     9781 agcataagtt tacatgtagc aactcagctt tacattctta tccagctgca tcatggtatt
     9841 atccagaagc ttctggaagt catctacctg attatagcgg tcccatttgg taggatcctg
     9901 ccatccgcaa tcctggtgcg gagtgtactt aacccttgac catctgaggt ctttcttgaa
     9961 gatcatggca aataccttgg tagcatcatc tttggtcata ctgacatcca gataataggt
    10021 ccactccaga tttcgcatga gagatgggaa tgacaccata ggctggctca ttgctgcatc
    10081 cttataaaag gtaagggtat aatagatcct ggcaccttct gtactcttgt attccaattt
    10141 ccagtacaat ggctttcccg ggttattcag ggaattctcc actttaccca tatggttggc
    10201 agacatgaaa tctttcatga ccacctgggc ctgagagcct aatgacaatg ctcctgcaaa
    10261 cagcagggta agcatcaact tcatagtagt tttcatctaa tgtatatttt cagaataatt
    10321 acttggcagc tacttcttca gacttcatcc ctttgcggtg ggtgtctgtt acgatcttgt
    10381 tcagaacata atcgccggca cactttcctt gttcaaggcc atccatgatc tcacaggtat
    10441 agtgaatgcc accatacatg cggctgtagg ctgcttcctg ggcagcatca cggaaagagt
    10501 taaaggtttt aggttccaga ccgatcttca cgtgggtact gtctgtgaaa ggcttgttat
    10561 caccaatcag ggcagtcatg acctcagcag ctgctccgga aaagcctgag tgaccggaag
    10621 tatagccagg gaacggaggt gtttccacat aaggttccca acccggttca atgttctctt
    10681 caatataggt cttgggacga accaggttca cacggtattt cgtgtgccac agagaaatac
    10741 aggcatcctg catggacact gctaccagtg catacatttc agcagctttt tccaggttcc
    10801 agttctctgc aagcacaaag ttacaggaca tattgatcca gtgacccgga ggtgtagctg
    10861 tttcacccgg atcatcagcc cagtactgag caacgatgcg ctgatcttcg gtcaggttct
    10921 tgtcaactgt atagacttcc atcgcttcct tatagaaatc agaattagga tctgtatcat
    10981 aagcgaactt catgtccaca tcacacattt tgttgtttgg aacagcaaat gttctgtggg
    11041 ttccccaaaa gggttccaga gcggtttggt tgaagtctgt aggttcccag tattcagggt
    11101 ggccttcacg gcttggagcc ttgtactgtt cataacgggt acgctcgtat ccgtccgttt
    11161 cgctccaggc aataatggca tcagctaacg cacggccata ctctttggaa cgcatcatca
    11221 cgtcttcagg aacagtggca gcacgctcac tgatctgctt ttcatgcagc ttgttgatag
    11281 cagccacacc tgcagggagg tagcgaccca gcatctcatc ctgtacataa taaacagatt
    11341 cgatggtcac tgtggtccag tcataaacct cgtccttatt aggcctgggc agatccttca
    11401 gctcattgag ctgaccttcc agtgacatgt aatccggagc tccgcatacc tgcgcttcat
    11461 acatagtggt acctatatag gcatacatgc gtgaagcatc gggcggcgag agtttctcgc
    11521 tggctacgaa gctgaaaagc tgtttccacc agtccaccag gaaagtaccg gattgatcgc
    11581 ttgaattctg agaaacactg ttgtacagcg gattggctac atcgctgctc tctttgcctt
    11641 tgcagcctgc ggcgagcagc atcccacccg cgagtacaag agcaaacact tgtttggtgc
    11701 tcattttaag ggagattttt tggtgtaaat gaaagtttaa cagggtcata aaaacaagac
    11761 gcaaaattaa ccgaaaaatg tggtttttcg gccttttaca aaacatttaa aggattaaca
    11821 aaacatttac ataagtgcaa atggtcaacc tgatgaggac cgtaattttg tgtcctcaaa
    11881 ttcaattcaa gacaacatga agtacgtcgt ttaccctgtg atcgccacga tgctgctaac
    11941 agccgggtgt tccaaaaaac aaagctttca ggatcggatg aacgagtgga agttcaccga
    12001 aaacatcgac caggctaagg tagtgccgct ggacggcaat accggcaaaa tgttcgacat
    12061 cctgaccgga gctaccaccg gtgtgaattt caacaacgag atcaaggaaa cctttgagct
    12121 caattactat cgctatggat attcctacaa cggtgccggt gtaggtgtgg gcgacgtcaa
    12181 caacgacggc ctgcctgacc tgtattttgc cggtaacgtg gtacctgacc gcctgttcat
    12241 caacaagggt aacctgcagt ttgaagacat cactgaatcc tgtggtatca atggcaaagg
    12301 tggttggtca acaggtgtca cctttgtaga tatcaatgaa gacggcttcc aggatatcta
    12361 tgtatgccgg gcccgctttg acgacgctga catgcgcaga aacctgctct atgtcaataa
    12421 cggtgataat acattcactg aaaaagcagc tgaatatggt atagatgacg atagttattc
    12481 tacccatgcc aacttctttg atgccgataa cgatggcgac cttgacctgt atgtggtaac
    12541 ccaccctaca gactttaaag acaaaaacaa gcagaaaaat ttccagaaga tcgaacaggg
    12601 tgtgaaccag tctaacaggt tctatcgcaa cgaaggaaat ggaaaatttg ttgaatgcca
    12661 ccaggaagtt ggtatcaata accacggttt cggcctgtct gtaaccgccg gcgacctcaa
    12721 caacgatggc tggcaggata ttttcgtagg taatgactac atcatgcacg attacaccta
    12781 catcaaccag ggtaacggaa cctttaaaga aagcagccgt gaactgctga ccaagaccgc
    12841 ctattacggt atgggtacag atgtagctga ttacaacaac gacggtctgc tggatatgat
    12901 gaccgttgat atggagattg aaggcaacta cggcaccaag actttcatgc agtctaacaa
    12961 acaaaccttc ctgcgtaccc tggtcaatgc cggttacctg cagcagtatg gccgtaataa
    13021 cctgcagctc aataacggta tgggtaaatt ctctgaagtg gccaactttg ccggtgtttc
    13081 caccacaggc tggagctggt ctcccctgtt tgccgactat gacaacgacg gaaacaaaga
    13141 cctctacgta accaatggct tcatgaaaga cagccacatg gatgtgatgg aagtctatat
    13201 caaactgacc agagcgaacc gcctctctga ctctactgac tactatgaat tgcggaaaat
    13261 gctgccggag aacagcgtac ttcagttccc taatgccatg ttccacaata caggcgatct
    13321 gacctttgac gatgtcaggg aagactgggg cctctatcat ccgtcgatga cctatggagg
    13381 tgcctatgct gacctggatg gcgatggcga tattgacctt atctgtaaca acgccaatta
    13441 tcctgccttc atcattcgta acaacgaaga caaaatgatg ggcaacaatt atctgcgtat
    13501 tgatctgaaa ggaaacaaaa gtaaccccga aggtctgggt gccaaggtct atgtgaccaa
    13561 tggcaatact actcagtatg ttcagctgga aactgtgaag ggttatatgt cctgctcaga
    13621 atctgttgct cacttcggta tgggcaaaaa cacaactgcc gataaggtag tggttcgctg
    13681 gctggatggc aaagagaacg tcatgaccaa tgtgcccgtt aaccagcttc tggttattga
    13741 ccataaagac gctgtgtaca acaaagtaag tgaagtgccg ggccagcagc ctttcttcgt
    13801 ggacgctacc aatgaactga agatcgatta tgtacacaag gaaaatgaat ttgacgactt
    13861 tgacagagag ttcctgattc ctcacgaact cagcaatatg ggccccggca ttgctgtagc
    13921 cgatgtgaac ggcgacgggc tggatgactt ctttgttggt ggagcccgca tgtctgctgg
    13981 tgtactgtat gtgcaaaaca ccaatggaac cttcagcagg tctcagggat ataacccagg
    14041 tgcagatgaa atagagtgcg aagacatggg tgccatcttc tttgatgcag acggcgatgg
    14101 agatcaggac ctgtatgtgg ttagcggcgg atctgacttc cctaaaaatg acccgcacct
    14161 gcaggatcgc ctgtacatga acaacggtgg caactttgcc ctcaacaaag aagcattgcc
    14221 tgctatgatg accagtggtt cttctgtggc agccaacgac tttgacaagg acggtgatat
    14281 ggacctgctc attggcggac gtcttgttcc cggcgactat cctgtagctg ccagtaccta
    14341 catcctgcgc aacaatggtg gtaaattcag tgacgtgacc gcagagatcg ctcctgacct
    14401 ggctaaagcc ggtctcgttt ctgctgtact ctggacagac ttcaataatg acaaccagac
    14461 ggatatcatc gtaaccggag aatggatgcc catcatggtc ttccagaaca agggtggaaa
    14521 attccagaac atcaccaaga atgccggact cgataagtcc actggctggt ggaacagcct
    14581 gaccgcaggc gactttgaca acgatggcga catcgactac atagccggca acttcggtct
    14641 gaactgccgc tataagccaa aagaaaaacc gcttgaactg ttctatgatg actatgataa
    14701 tgatggtcgt catgacatca tcatgtgcta ctaccagaag gataaactgt acccgatcaa
    14761 aacccgtgag cgtatgatcg aacagatccc gtccattgca gaaacattcc ctaactggga
    14821 tagctacgga cgtgctgaag tgtgggattt ctacggtaag gaacgcatgg aaaaatctca
    14881 gggacattac accgctgagt cattcaacac ctcttatatc gaaaacctgg gcaacggaaa
    14941 atttgcgatc aggaaactca acaacgtgat gcagatctct accgtattcg gaatggagac
    15001 catggatctg aacggtgacg gattcctgga tctcatagcc cacggtaact ggtatgaaat
    15061 ggagatcgaa accaacattc aggacgccgg tatcggtaat gtcctgcttg gaaatggcga
    15121 tggcacattt accccggtac acagccgttt cagtggcttc tactctccgg gtaacgccaa
    15181 aggtcttgcc aaactgaccg ttggtaaaga taagaccccg gtcatcctgg ccagcaacag
    15241 caacgacaag atgtatgcct ttaaatatat aggcaaacac cgcactgtta ccctgcaggc
    15301 agatgacagc tatgccatcg tgaccatgaa tgacggaaag cagagaagaa tggaattcta
    15361 tacaggtgcc agctaccttt cacagagtga taagttcatg ctggttaccc ctgaaatgaa
    15421 atctgtaatt gtctataaca acaaaggaga aagcagagaa ctcaccgcca atggttgggt
    15481 agctgttaaa taataaggaa aaagaataat caagaaatga aaaatcctat agcaaagatc
    15541 tcgttgctta gtttgtctgc cggactgatg ctcatcagtt cctgcaagct cttcagcggc
    15601 ggcgacagcg ataccagagg atccgtgatc attgataagg caagagaagt gacgctggat
    15661 ggaaacagtg gaaaacgctt tgatatcctg accggaaaat caacaggaat tgacttcgtc
    15721 aacgacctga ccgagaacta cgaagtaaac tggtggagat attcctatat ctacaacggt
    15781 ggcggcgtat gtattggtga tgtcaacaaa gacggcctgc cagacctgtt ctttaccggt
    15841 aacctggttc ctaacaagct ctacatcaac aaaggcaata tgcagtttga agatgtgacc
    15901 gcccagtcag gtatcgttaa ggaagaatgg gagttcagct acggtgctac catggttgac
    15961 tttgacggcg ataatgacct cgacatctac atctgtaact cccgctggga caaaccggaa
    16021 aaaagaaaga accggt
//
LOCUS       DAOMAX010000030        15819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_411_length_15819_cov_3.895902, whole genome shotgun sequence.
ACCESSION   DAOMAX010000030 DAOMAX010000000
VERSION     DAOMAX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15819
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_15819_cov_3.895902"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PK742_03030"
     CDS             <1..946
                     /locus_tag="PK742_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQU75591.1"
                     /translation="RGLKDRMRIMEKPQVTIGEDDLTGKNSVLVEMVITANSGFEGKT
                     LGDLDFRRRYRGVPLAIRHREEVLHEHLYDVELKAGDIILAEVKRHFMSELNNDEQER
                     NADFILLSEDEVPDFRKKRFLVTLGIVLTVIVLASTNVLDIMTGAIAATSLLVLIGFL
                     KMKEVYESINWNIIFLLAGALTLGTAMENSGLDLMIASGLVNNLGHLGPVAIISGLYL
                     VTSILTEIMSNTATAALIAPIAISTAATLGLDAMPFVMAVTFAASASFSTPVGYQTNT
                     MVYSAGNYRFKDFLRVGIWLNLFFWLLATLLIPLIYPV"
     gene            complement(1087..2472)
                     /locus_tag="PK742_03035"
     CDS             complement(1087..2472)
                     /locus_tag="PK742_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU75592.1"
                     /translation="MKKWSLMLFFLLAGFVTFAQTSKITSAITYLENGELDRALAAIE
                     EATVHEKTAGIAKTWFTRGRIFNAIAADESGKFAGLSENPLDMAMESLNKALTLPDAD
                     KYKKQMLVEYSLLQMNYFNMGSGSYGQRDFQGAHDNFRKSADANKKLLEIDPTLPLDT
                     GVIFNIGLTAYRIGNRDEAIDIFQKLVNMKYKEPYVYQALAEMYAEDGKMQEAFDVIA
                     TGRKNYPNDEALIITELNFYLSQGKVEEIVDKLNEAIAVDPNNEELYFAKGNAYGELM
                     KTAVIEGGSPSASVKACLGAPDAMEAMEGKHEGAEVWTYGKVKFVIEEGKVYEIEGAD
                     KDIKALTGKCNDREKYNAYFSEAVGAYRKAIDINPGNFDNYLNLGALYYNTAIEINKK
                     MINLPLDADKEYAELESRRNELYKEALPYFEKALEIDPENIPTMQALKEIYAKTNNFE
                     KMKEIKAKLGE"
     gene            complement(2520..5054)
                     /gene="gyrA"
                     /locus_tag="PK742_03040"
     CDS             complement(2520..5054)
                     /gene="gyrA"
                     /locus_tag="PK742_03040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQU75593.1"
                     /translation="MAEGERIIPINIEEEMKTAYIDYSMSVIVSRAIPDVRDGLKPVH
                     RRVLFGMQELGVNYNKPYKKSARIVGEVLGKYHPHGDGSVYDAMVRLAQDWSMRYTLV
                     DGQGNFGSVDGDSPAAMRYTEARFQKITEEMLTDIDKETVDFSNNFDDTLEEPTVLPT
                     KFPNLLINGSSGIAVGMATNILPHNLSEVCDGIVAYINNREITTQELMAYIKGPDFPT
                     GGQIYGVEGISEGFETGRGRIVVRGTADIETMKNGREQIIVTAIPYQVNKAMLIAKTA
                     ELVNEKRIEGISDIRDESNREGMRIVYELKRDAVASVVLNQLFKYTPLQTSYGVNNIC
                     LVGGRPRLLGLKELISYFVEFRHEVVVRRTQYDLRKAQERAHILEGLLIALDNLDEVI
                     NLIRSSKTPDDAKEGLMTRFGLSDIQSKAILEMRLQRLTGLERDKIRAEYEEVMALIA
                     RLEEILANEGLRYQIIVDETLEMKEKYGDERKTSIVAAEGELSIEDLINEEDVVITIS
                     HMGYIKRTAATEYRVQGRGGKGFRGVAARDEDFIEHILIASTHDTMLFFTQQGKCFWL
                     PVYQIPEGNRTSKGRAIQNMISLPPGDKVMAYVDVHDLKDEEFLNNHFLVFCTRKGMI
                     KKTALEAYKRPRANGIAAITINEGDELLEVKLTNGHCEIMMGVKSGRAIRFPEEKVRP
                     MGRTAAGVRGVHLAGDDDEVVGMVSVEKGDRERTILVVSEHGYGKRSYLVDPEDGEDV
                     YRVTNRGGKGVTTIKVTQKTGQLIAVKSVTDNDDLMIITVKGITIRMRVDDIRVMGRA
                     TQGVRLINLKDGDSIASITKAEREEETEEQVEGEGAEGSDTEPTNE"
     gene            complement(5158..7233)
                     /locus_tag="PK742_03045"
     CDS             complement(5158..7233)
                     /locus_tag="PK742_03045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQU75594.1"
                     /translation="MRKILFAATLGMSLSLCAQNVMTPETLWSLGRVGAETLSADGQA
                     LIYRVTYFDAAANNSESNLYRLELDGGKITPLSSGKGTEYSVMTLPDGSMGYLYKGQI
                     WKGDWNTGNATQWTNYEPGLDNVRFSPDGTRILFTGEVKTGTTVQDRYADLDQANAYV
                     LDDLMYRHWDHWEDAFSSHVFVADMASNGTISNVRDIMYGEPYDCPQQPFGGAEDVIW
                     NHDGSAIYYVSKKSTGKAYAISTNSDIYAYDLASGMTTNMTEGMMGYDTNPQVSPDGQ
                     WMAFLSMERDGYEADKNRMMVKNLQTGELRDLSTFLDESVASISWSNDGKKIWFIAPV
                     KGVEQLHAFDFDSYIRGLRIFPITPVVEGQFDITGIVGEQDGVLFVTRTDMNHATEVY
                     SLALKTKDMRQVTHVNDKIYNSLAQSRVEPHWIKTTDGKDMLVWLILPPNFDPNKKYP
                     SLLYCQGGPQGVLSQFYSFRWNFQLMAAQGYVVIAPNRRGMPGHGVEWNEEISKDWGG
                     QAIQDYLSAADWGKTLPYVDGSRMGAVGASYGGYSVYMLAGVHEGRFSSFIAHDGLFN
                     LQSFYGTTEEMWFANWDLGGPYWENGVQDKSYDQFNPMNYVANWDTPILIYQGGRDYR
                     TTEDQAFQAFNAAQLRGIKSRFVYMPEENHWVLSCQNGLLWQREFFRWLDETMPSAMQ
                     YKPKGAKEE"
     gene            7405..7884
                     /locus_tag="PK742_03050"
     CDS             7405..7884
                     /locus_tag="PK742_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQU75595.1"
                     /translation="MSQQLSTKVAEVLKNRFHTLAGHNRPTAEEVLAMPVESLRGIGF
                     SYAKAGYVHAVATFSLEEDLSRRRLNRMDDEEVIAHVTRIKGVGKWTAEMLLMFCLGR
                     PDVMPVDDLGIQQAMTRLYDLDPADSKQLKKDMLFIAERWRPYRTYACMHLWNWKDQ"
     gene            complement(7885..8577)
                     /locus_tag="PK742_03055"
     CDS             complement(7885..8577)
                     /locus_tag="PK742_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQU75596.1"
                     /translation="MDGKEHTILITGLSGALGKVVGGRLLNEGHRLLAIVRNESSENR
                     TKADFPGMENRISFFRADLTTDTFEGLSTEDLSGFVHLAGGFDAAPTFAEAPADQLEK
                     QFLLNTRSAFQLLRLVLPALKKRGGGSIVTISAKAALQPDGSNAAYAASKAALLNLTL
                     QAAREGRKENVRANVIVPGVIDTPANRSWGSADQIAKWTTPEDIASCICWLMSDASAG
                     VNGTVIPMYGQL"
     gene            8716..9408
                     /locus_tag="PK742_03060"
     CDS             8716..9408
                     /locus_tag="PK742_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQU75597.1"
                     /translation="MYITLLQITTDTIPSVSDLAAEPSEKLSLIELAFKGGIIMIPIA
                     FLLLLTVFIFFERYFTIRKAARGDKNFMLNIRDYISNGNIEAAKMLCKNTETPYARMI
                     EKGIMRIGSPLKNIEVAVENVGKLELYRMEKNLAILALASGIGPMIGFLGTVTGMIRA
                     FYDMSVSDQISPKLLSSGIYEAMVTTVAGLIVGIIANLAYNSLLSLVEKVIYKMEATT
                     VEFMDLLQEPAA"
     gene            9408..9806
                     /locus_tag="PK742_03065"
     CDS             9408..9806
                     /locus_tag="PK742_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQU75598.1"
                     /translation="MALRSRNTRKAEFSMSSMTDIIFLLLIFFLLTSTLVAPNAIPML
                     LPNSNTTAQATETLAVSIDRDLNFFVNKREVSVDDLEAVLTANLKGTKDEGIVLYADK
                     SVPVDEVVKVLNIANTLKIKVVLATNPDKK"
     gene            9852..10640
                     /locus_tag="PK742_03070"
     CDS             9852..10640
                     /locus_tag="PK742_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75599.1"
                     /translation="MATVYAYEKKAKRRSVLLTVLFAAGVLAILILAGFRPPFPPLEP
                     EGLLIDFGFDESGSGKIESASRISAAPQAQNQPVEQKIETQDFQETVTLPEEKKKTDK
                     PKTETKTDTKKDPEPVIDQGEVFNPNKHKFDSNSSSDGSSGGKGNEGHIDGNPNGYPG
                     NGSGAGDNGTNGFGYDLSGRSMVSKPVIGGTPPESGKVVLKITVDRNGVVTTAVYERK
                     GSTIVDQGVINEAIRSVKGKKLFNTSSDAPETQTGTLTINYTLK"
     gene            10699..12003
                     /locus_tag="PK742_03075"
     CDS             10699..12003
                     /locus_tag="PK742_03075"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQU75600.1"
                     /translation="MTYQETIDWLMQRLPMFQRIGPAAIKKDLTNTIRLCEALGHPEQ
                     AFSSVHIAGTNGKGSVSNWLSAILQSSGHQTGLYTSPHLLDFRERIRINGVLCEEAFI
                     TDFVSRYRPVIEEIEPSFFEITVAMAFLYFAEKGVDIAVIETGLGGRLDSTNVITPVL
                     GIITNIGWDHMAMLGNTLPLIAAEKAGIIKPGVPVLIGERQEEVQGVFKEMAVLQGAP
                     LYNSDNLVRLEVLSNDPAGLCFRAQLETTQFTLETCTPMFGSYQEKNLQTVLAAADLL
                     QQDWQISVNDIISGLQQVKELTGFRGRMEILQHQPLVLADCAHNSDGLDQLRRSIDAM
                     DVPHWHIVTGAVQDKDVAANLLHLPESASYYFCKPDIPRGMDARTLQQLAANMERKGV
                     AYGSVHEAIVSALSNSGSDEAILICGSIFVVAEALPNFKHKT"
     gene            12000..13418
                     /locus_tag="PK742_03080"
     CDS             12000..13418
                     /locus_tag="PK742_03080"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQU75601.1"
                     /translation="MKRLLIPVLLALVLSVLTQPVMAQKGYDYQVTIRGMEDTVLYFG
                     HHYGNKQYVIDTVRTDKNGTAHFAGQEPLPGGVYIVVMPKMNNKYFEMIVNEPAFEMA
                     TDTTDITGHMQVTGSVENQVMYDDLRFIGDRRSRVNVLRKEQEAAEQGSKRAEKIQEE
                     MDLINQEVLDWRNNLYAKYPGTFYVKLLKASADLTLPEAPVLPDGTPDKKYLIQWVHE
                     HYFDDIDLKDSRLLRSSVLHMKVEQYLNTYVPKIPDSVSAAIDRIIEGARGNNEVFQY
                     LVVTLLNQYASSDIMGMDAVFVHMVDKYYKSGDAFWLDDAALFRITNKAALMKPTLIG
                     KQAPPIMLQDSAGIDIPLYSIDSKYTVVVFWDVDCGHCKKEMPKLVEAYPKIKELGAE
                     VYAVYTEEEWDKWKDWLDEKHYNWINVGNVKRESNFQALYNVDQTPLLFILDKNKKII
                     GKHLKAEQLYDFLKNYQEFNDL"
     gene            13408..13848
                     /gene="msrB"
                     /locus_tag="PK742_03085"
     CDS             13408..13848
                     /gene="msrB"
                     /locus_tag="PK742_03085"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQU75602.1"
                     /translation="MTYKHLKINKRDSFRPVLQKSDKEWELELSQDQYHILRNKGTER
                     PFTGAFDAHFEPGKYVCAGCGTPLFYSGAKFNSHCGWPSFDAPATDENITYLKDTSHG
                     MIRIEIVCSNCGGHLGHVFDDGPTDTGLRYCVNSASLLFKKEED"
     gene            complement(13849..14898)
                     /locus_tag="PK742_03090"
     CDS             complement(13849..14898)
                     /locus_tag="PK742_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75603.1"
                     /translation="MKNPSPRQIVLRATLLVVLLVVVLEVIPSLLPVLNDFRYHLLLV
                     PILMFGLTYLIFYRVVENFIYRKIKLIYKNIHNLKTTKNVVESRLDLSTDIIGAVNKE
                     VLNWAEASRKEISQLRSMENYRKEYLGNVSHELKTPITSIQGYLETLIDGGINDPRIN
                     MDYLSKAIRNVERMVSIINDLESISHLESGQLKLQMQPFDMNALTKEVFSDLEIQAAQ
                     RNIRLKIKEGCDRVFMVEADKKMIREVLVNLITNSIKYGKDNGQTNVGFYNMGDNILV
                     EASDTGIGIGKEDLSRVFERFFRVEKSRSRDFGGTGLGLSIVKHVIEAHNQTITVRST
                     PNEGSTFAFTLRKAD"
     gene            complement(14898..15578)
                     /locus_tag="PK742_03095"
     CDS             complement(14898..15578)
                     /locus_tag="PK742_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU75604.1"
                     /translation="MSSKIKILIVDDEPDVLEFIEYNLTREDYDVVKASNGKDALKMA
                     ASEKPSLILLDIMMPEMDGIEVCRELRSNNDYDNTIIAFLTARNEDFTQVQGFDVGAD
                     DYITKPIKPRLLISRIKALLRRTAADLQNSGQQQFGDLIIDREKFLVFRKGEPINLAK
                     KEFELLQLLTSKPGRVFTRQEIFNDIWGMDVIVGDRTIDVHIRKLREKIGEEYIKTIK
                     GIGYKFDY"
BASE COUNT         3965 a         3905 c         3673 g         4276 t
ORIGIN      
        1 ccgcggacta aaggaccgca tgcgcatcat ggaaaaaccc caggttacca tcggcgaaga
       61 tgatctgaca ggcaagaaca gtgtattggt agagatggtc atcactgcca actcaggctt
      121 tgaggggaaa acgcttggag acctggattt ccgccgaagg tatcgtgggg tgccactggc
      181 tattcgtcac cgggaagaag tgcttcatga acacctgtat gatgtagagc tgaaggccgg
      241 cgatatcata ctggcagagg tcaagcgaca cttcatgagc gagctgaaca atgatgagca
      301 ggaacgcaat gcagatttca tcttgctgag cgaagatgaa gtgcctgatt tcagaaaaaa
      361 gcgttttctg gtaacactgg gtatcgtttt aactgtgatc gttctggcca gtaccaatgt
      421 gctcgatatc atgacaggtg ccattgcagc aaccagtctg ctggtgctga tcgggttcct
      481 gaagatgaag gaggtgtatg aaagcatcaa ctggaacatc attttcctgc tggcgggcgc
      541 tttgacgctg ggtactgcta tggaaaacag tggcctggac ctgatgatcg ccagcggact
      601 ggtcaataac ctgggtcacc ttgggccggt tgccatcatt tctggtctgt acctggttac
      661 cagtatcctg actgagatca tgagcaatac cgccacagcg gctttgatcg cacccattgc
      721 catcagtacg gctgcaaccc tgggtctgga tgccatgccg ttcgttatgg cggtcacctt
      781 cgctgcatca gcaagtttca gtacgcctgt cggctaccag accaatacca tggtctatag
      841 tgccggcaat tatcgcttca aagatttcct gcgtgtaggc atctggctga atctgttctt
      901 ctggttgctg gccactttgc tgatcccgct catttatccg gtttagttgc gcataaggcc
      961 tgtattgtaa caagttataa aagaaagagc tgcagactga aatgacctat tctgtcctat
     1021 aattaatatt atgttaagta gtagtcaata aaataagggg gatctgcttc ccccttattc
     1081 tgttatttat tcgcccagtt tggctttgat ctctttcatc ttttcaaagt tgttggtctt
     1141 cgcatagatc tctttcagtg cctgcatggt cggaatgttt tccggatcta tctccagtgc
     1201 tttttcaaaa taaggcaaag cttccttgta cagttcatta cgtctgcttt ccagctctgc
     1261 gtattcctta tcagcatcca gcggcagatt gatcatcttc ttgttgatct ctatggcggt
     1321 gttgtaatac aaagcgccca ggttcaggta attgtcgaaa ttacccggat tgatatcgat
     1381 cgccttgcgg tatgcaccaa cagcttctga gaaataggca ttgtattttt ctctgtcatt
     1441 gcatttgccg gtcagggctt tgatgtcttt atcagcacct tctatttcat ataccttacc
     1501 ctcctctatt acaaacttca ctttgccata ggtccagacc tcggcacctt catgcttacc
     1561 ttccatggct tccatggcat caggagctcc caggcaggct ttaacactgg ctgatgggct
     1621 tcctccttct atgaccgctg tcttcatgag ctcaccatag gcattgcctt tggcgaagta
     1681 cagttcttca ttattcggat ctaccgcaat ggcttcattc agtttatcta cgatctcttc
     1741 cacttttccc tgactcaggt aaaagttcag ttctgtgatg atgagggcct catcattcgg
     1801 ataattcttg cggcctgtag cgatcacgtc aaacgcttcc tgcattttgc catcttcagc
     1861 atacatttcg gccagagcct gataaacata aggctctttg tacttcatat tgaccagttt
     1921 ctggaaaatg tcaatggctt catccctgtt tcctatccgg taggcggtca gtccgatgtt
     1981 gaagatcacg ccggtgtcca gcggcaaggt cgggtcaatt tccagcagct ttttgttggc
     2041 atcagcactt ttcctgaaat tgtcatgagc accctgaaag tcacgctgtc cgtaagatcc
     2101 tgaacccata ttaaagtagt tcatctgcaa caggctgtac tctacgagca tttgcttctt
     2161 gtacttatct gcatcaggca aggtcaacgc cttattgagc gattccatgg ccatatcgag
     2221 cggattttct gacaatcctg caaattttcc gctttcatcg gccgctatgg cattgaatat
     2281 ccgccctctg gtgaaccagg ttttggctat tcctgctgtt ttttcatgca cagttgcttc
     2341 ctcaatagct gccagagcac ggtccagctc gccgttctcc aggtatgtaa tggctgaagt
     2401 gatcttgctg gtctgggcaa atgtcacgaa acctgccagc aggaaaaaca gcatcaaact
     2461 ccattttttc attttcttat tctggtttaa gtgttttatt tacaactaat tacgatcttt
     2521 tattcatttg ttggttcagt atcggagcct tctgcaccct caccttccac ctgttcttcc
     2581 gtttcctctt cccgctcagc cttggtgatg gatgcaatgc tgtctccatc cttcaggttg
     2641 atgagtctca caccctgggt agcccttccc atcacacgga tgtcatccac ccgcattctg
     2701 atcgtgatgc ctttcacggt tatgatcatc aggtcatcat tatctgtaac gctcttaact
     2761 gcgatcagtt gcccggtctt ctgggtcacc ttgatggtgg tcaccccttt tccgcctctg
     2821 ttggtcaccc ggtacacatc ttcaccgtct tcagggtcca ccagatagct tctttttccg
     2881 tatccatgct cactgaccac gagtatggtg cgctctctgt ctcccttctc tacagaaacc
     2941 ataccaacca cttcatcgtc atctccagca aggtgtaccc ctctcacccc ggctgcggta
     3001 cgacccatcg gacgaacctt ttcttcgggg aagcggatgg ccctgcccga cttcactccc
     3061 atcatgatct cgcaatggcc gttcgtgagt ttcacttcca gcagttcatc gccttcattg
     3121 atggtgatgg ctgctatacc gttcgccctg gggcgtttat aggcttccag agcggttttc
     3181 ttgatcatcc cttttctggt acagaacacc aggaagtggt tgttgaggaa ttcttcgtcc
     3241 ttcaggtcat gtacatccac ataggccatg accttgtcgc cgggaggcag actgatcata
     3301 ttctgaatgg cccgtccttt ggaggtccgg ttaccttccg gaatctgata taccggcagc
     3361 cagaagcact tgccctgctg cgtaaagaac agcatggtgt catgggtact ggcgatcaga
     3421 atatgttcta taaaatcctc atcacgggct gcaactcccc ggaaaccttt acctcccctg
     3481 ccctgaaccc ggtattcggt ggcagcagta cgcttgatat agcccatatg agatatggta
     3541 atgaccacat cttcttcgtt gatcaggtct tcgatggaaa gttctccttc agctgctacg
     3601 atgctggttt tgcgctcatc accgtatttt tccttcatct ccagggtctc atccacaatg
     3661 atctgatagc gcagtccttc attggccagg atctcttcca gacgggcgat cagtgccatc
     3721 acctcttcat actcagccct gatcttgtca cgctccagac cagtaagcct ttgcagtcgc
     3781 atctccagga tagcctttga ctgaatatca gacaagccga aacgggtcat cagaccttct
     3841 ttggcatcat ccggggtctt ggatgaacgg atcaggttga tcacttcgtc cagattatcc
     3901 agagcaatga gcagaccttc cagaatatgt gcacgttcct gtgccttgcg gaggtcatac
     3961 tgtgttctgc gaaccaccac ttcgtggcga aactcaacaa agtaactgat gagctctttc
     4021 aggccaagca gcctcggcct gccaccaaca aggcaaatat tattgacgcc gtagctggtt
     4081 tggagcggtg tatatttgaa taattggttg agaacaacag acgcaactgc atcacgctta
     4141 agctcatata caatgcgcat gccttcgcgg ttgctctcat cacgaatgtc tgaaatacct
     4201 tcaatgcgct tctcattgac cagttctgct gtcttggcga tgagcatagc cttgttgacc
     4261 tgataaggta tggcggtaac gatgatctgt tcccggccat ttttcatggt ctcaatatct
     4321 gcagttcctc gaacgacgat ccttccgcga ccagtttcaa agccctcact gatcccttct
     4381 acaccataga tctgtccacc ggtgggaaag tccgggcctt ttatataggc catcagttcc
     4441 tgggtagtga tctctctgtt attgatatag gccacgatac catcgcatac ttcactcagg
     4501 ttgtgcggta gtatattggt agccataccc acggcgatac cactggagcc attgatgagc
     4561 aggttgggga atttggtggg caacaccgta ggctcctcca gcgtatcatc gaagttgttg
     4621 gaaaagtcaa ccgtctcttt atcgatatcg gtcagcatct cttcggtgat cttctggaat
     4681 ctggcttcgg tatatcgcat ggctgccggt gaatctccat ccacagaacc aaagtttccc
     4741 tgtccgtcca caagcgtata gcgcatgctc cagtcctgag ccagacgcac catagcgtca
     4801 tagactgagc catcgccgtg cgggtggtac ttacccagca cctcaccgac tatacgggct
     4861 gatttcttat aaggtttgtt gtaattgacc cctagctcct gcatgccgaa cagcaccctg
     4921 cggtgtacag gtttcaatcc gtcacgaaca tccgggatgg cccgtgacac aatgaccgac
     4981 atcgaataat cgatgtaagc ggtcttcatt tcttcctcga tatttatcgg gataatccgt
     5041 tctccttctg ccatttttaa tgcttcctgt aagcatggca aaactacgaa aatgaggggt
     5101 caaatggcgc tttttaccct tgcgaaaacg gtcaagtttt caacattaaa acctgcctta
     5161 ttcttctttg gcgcctttgg gtttatactg catggctgat ggcatggtct catccagcca
     5221 gcggaagaat tcccgctgcc aaagcaggcc gttctgacaa ctaagtaccc agtgattctc
     5281 ttccggcata tacacgaatc tgctcttgat gcctctgagt tgcgctgcat tgaatgcctg
     5341 gaaagcctgg tcttcggtag tgcggtagtc gcggcctccc tgatagatca ggatcggggt
     5401 atcccagttg gctacgtagt tcatggggtt gaactgatca tagctcttgt cctgcacgcc
     5461 gttctcccag taagggcctc ccagatccca gttggcaaac cacatctctt ctgtggtgcc
     5521 atagaatgac tgcaggttga acaacccgtc atgcgcaata aatgaactga atctgccttc
     5581 atgcaccccg gccagcatat acacggaata accaccgtag ctggcaccta cagcacccat
     5641 tctgctgcca tccacatacg gcagtgtctt gccccagtct gcagcactca ggtaatcctg
     5701 aatggcctgt ccgccccagt ccttgctgat ctcctcattc cattccacac cgtgacccgg
     5761 catccctctt ctgttcgggg ctatgaccac ataaccctga gcagccatta gctgaaagtt
     5821 ccacctgaag ctatagaact ggctgagcac tccctgcggt ccaccctggc aatacagcaa
     5881 agacggatat tttttgttcg gatcaaagtt tggtggcagt ataagccaaa ccagcatatc
     5941 ttttccatcg gtggtcttga tccagtgcgg ttctaccctg ctctgtgcca aactgttgta
     6001 tatcttatca ttgacatggg tcacctggcg catgtctttg gtctttagtg ccagtgagta
     6061 cacctctgtt gcatggttca tatccgtgcg ggtcacgaat aatacaccgt cctgctcgcc
     6121 aacgataccg gtaatgtcaa actgaccttc caccacaggt gtaataggga aaatgcgcaa
     6181 tcctcgtatg taactgtcga aatcaaatgc atgcagttgc tccacacctt ttacaggggc
     6241 aatgaaccag atcttttttc catcattact ccagctgatg gaagctacag attcgtcgag
     6301 gaaagtgctc aggtcgcgga gttcgccggt ttgcaggttc tttaccatca tccggttctt
     6361 atcggcttca tagccatctc tttccatgct caggaacgcc atccactgtc catctggtga
     6421 tacctgcgga ttggtgtcat atcccatcat gccttcggtc atgttggtgg tcataccgga
     6481 agccaggtca taggcataga tatctgaatt ggtactaatg gcataagctt tcccggtgct
     6541 tttcttggat acgtaatata tcgcagaacc atcgtgattc catatcacat cttcggcgcc
     6601 accaaagggt tgctgcggac aatcataggg ctcgccatac atgatgtcac gaacgttgct
     6661 gatggtgccg tttgatgcca tatcggccac aaaaacatgg ctgctgaatg catcttccca
     6721 atggtcccag tggcggtaca tcagatcgtc cagcacataa gcatttgcct gatccagatc
     6781 agcatatctg tcctgaacag tggtacctgt ttttacttca ccggtaaaca ggatcctggt
     6841 gccatccgga gagaacctga cattgtcgag acccggttcg taattcgtcc attgtgtggc
     6901 attgccggta ttccagtcgc ctttccagat ctgtccttta tataaatatc ccatcgagcc
     6961 atcaggaagt gtcatgacac tgtattcggt tcctttacct gagctgagcg gagtgatctt
     7021 tcctccgtcc agttccagcc tgtacaggtt ggactcgctg ttgttggcag cagcatcaaa
     7081 ataggtgacc cgatagatca gtgcctgccc gtcagcactc agggtctcgg cccctacccg
     7141 gcccagcgac catagtgttt ccggggtcat tacattctgg gcacacaacg aaagcgacat
     7201 acctaatgta gctgcaaaca ggatcttgcg catcggatat tttttttcaa aggtatcaat
     7261 tgcagggatt atgccgattt ttaagcgatg gaatatctgc atcatctgca aaaggacaaa
     7321 caactcagac cggcacttaa aactggtggg ccatttgcct tgaagtcaaa aggtgaccct
     7381 tacctgtatc tgatgtacag cattatgagt caacagttat caaccaaagt agctgaggtg
     7441 ttaaaaaaca gatttcacac attggcgggg cacaacagac ccacagctga agaggtcctg
     7501 gctatgccgg tagaatcttt gagaggcatc ggattctcct atgccaaagc gggctatgta
     7561 catgcagtgg ctacattctc actcgaagaa gatctaagca ggcgaagact gaacagaatg
     7621 gatgatgagg aggtcatagc acacgtgacc cgcattaagg gagtgggaaa atggacggca
     7681 gagatgctgc tgatgttctg cctgggaaga ccggatgtaa tgccggtaga tgatctgggt
     7741 attcagcagg cgatgacccg cctgtacgac ctggatccgg ccgatagcaa acaattgaag
     7801 aaggatatgc tgttcatcgc agaaagatgg aggccctacc gtacatatgc ctgtatgcat
     7861 ctttggaact ggaaagacca gtgatcagag ttgcccgtac atcgggatga ccgtaccatt
     7921 cacaccggca gatgcatcgc tcatcagcca gcagatacag gaagcaatgt cttcaggtgt
     7981 tgtccattta gcgatctggt cggcactacc ccagctgcgg ttagcaggtg tatcgatcac
     8041 accgggaaca atgacattgg cccttacatt ctctttgcgt ccctctctgg ccgcctggag
     8101 ggtcaggttg agcaaagcag ctttgctggc tgcgtatgca gcgttgctgc catcgggttg
     8161 cagggctgct tttgctgaga tggtaacaat gcttcctcct cccctttttt tcaacgcagg
     8221 taagacaaga cgaagcagct gaaaagcaga acgggtgttc aaaagaaact gcttttctaa
     8281 ttggtctgcc ggagcctctg caaaggtagg tgcagcatca aaccctcctg ccagatgtac
     8341 gaatccggac aggtcttctg ttgacaatcc ttcaaaagta tcggtggtca gatcagcccg
     8401 aaagaaggat attctgtttt ccattccggg aaaatcggct tttgtacggt tttcagaaga
     8461 ttcgttccgc acaatggcca gcagtctgtg accctcgttc agcaaacgac ctccgaccac
     8521 tttacccagc gctccggaaa gacctgtaat aagtatggta tgttctttgc cgtccatgct
     8581 tgcgaaggta aaccaattca cttgttccac gtccacaata aaatacaatc acccctgtgt
     8641 gcgttagaag gcaaagcttt caggcagacc acttccctat ttttgttttc catcaaagaa
     8701 cactaaacaa cctgtatgta tatcacactc ctgcagatca ctaccgacac cataccctct
     8761 gtaagcgacc tggctgcaga gccgtcagag aaactgtcgc tcatagagct tgcattcaaa
     8821 ggcggtatca tcatgatacc cattgccttc ctcttgctgc ttaccgtttt catctttttt
     8881 gaaaggtatt ttaccatacg caaggcagct cgtggcgata agaacttcat gttgaatatc
     8941 agagattata tctccaatgg aaatatagaa gctgccaaga tgctttgtaa gaatacggaa
     9001 acaccctatg cccgcatgat cgaaaaaggc atcatgcgca tcgggtcgcc gttgaagaat
     9061 attgaagtgg ctgtagagaa tgtgggcaag ctggagctgt accgcatgga gaagaacctg
     9121 gccattctgg cactggcttc cggtataggg ccgatgatcg gatttctggg aacggttacc
     9181 ggtatgatcc gggcctttta tgacatgagc gttagcgatc agatcagtcc gaagttgttg
     9241 tcatccggta tatatgaggc catggtaacc acggtagcgg gtctgatcgt aggaatcatc
     9301 gccaacctgg catacaactc tttgttgtcg ctggtggaga aggtgatata caaaatggag
     9361 gcaaccaccg ttgaattcat ggatctctta caagaaccgg cggcataatg gcactacgat
     9421 caagaaatac cagaaaagca gaattcagca tgtcgtccat gactgacatc atctttctgc
     9481 tgctcatctt tttcctgctg accagtaccc ttgtggcacc gaatgccata cccatgttgt
     9541 tgcccaacag caatactaca gctcaggcaa cggaaacact tgcggtgagc attgaccgtg
     9601 atctgaattt ctttgtcaac aagcgggagg tttctgtgga tgatctggag gcagtactga
     9661 ctgctaatct gaaaggtacc aaggacgaag ggatcgtact gtatgcagat aagtctgtac
     9721 cggtagatga agtggtcaaa gtattgaaca tagccaatac gctgaagatc aaggtggttc
     9781 tggccaccaa tccggacaag aaataattat ggaaatgcgt agaattcgca tcctacagga
     9841 aatcaaagac tatggcaaca gtgtatgcat atgagaaaaa ggcaaagcgc agaagcgttc
     9901 tgcttactgt gctgtttgct gcaggagttt tggctattct gatcctggca ggttttcgtc
     9961 cgcccttccc tcctctggag ccggaaggac tgctgatcga tttcggcttt gatgaaagcg
    10021 gttcaggcaa aatagaatct gcttcgcgga tcagtgcggc accgcaggca cagaatcagc
    10081 ctgtcgagca aaagatcgaa acccaggatt tccaggagac cgtaaccttg ccggaagaaa
    10141 aaaagaagac cgacaagccc aaaacggaaa ccaagacaga taccaagaag gatccggaac
    10201 ctgtcattga tcagggtgaa gtgttcaacc cgaacaagca taagtttgac agcaacagct
    10261 ccagcgatgg ttcatcggga ggaaaaggta acgaaggtca tatagacgga aatcccaacg
    10321 gctatcccgg caacggttcc ggcgccggtg ataacggtac caacggcttc gggtatgacc
    10381 tgagcggacg cagtatggtc agcaagcctg tcatcggagg aactcccccc gaatccggaa
    10441 aggtcgtgct gaagatcact gttgacagaa acggtgtagt taccacagca gtgtatgaac
    10501 gaaaaggctc caccattgta gatcagggtg tcatcaatga agctattcgt tctgtcaagg
    10561 gtaagaaatt attcaacacc agctctgacg ctcccgagac acagaccggt actttgacca
    10621 tcaactacac actgaaataa attcataata ttcagaaaaa aggggctgca ccgagcagtc
    10681 cctttttatt tttgtggggt gacctaccag gagaccatag actggctgat gcaaaggctt
    10741 cccatgttcc agcgcatcgg accggcagct attaaaaaag acctcaccaa taccatcaga
    10801 ttatgtgagg ccttaggaca tccggaacag gcattcagtt cggtgcatat tgcaggaaca
    10861 aacggtaaag gttcagtatc caactggttg tctgccatcc tgcagtcatc gggtcatcag
    10921 acaggcctct acacctctcc tcacctgctc gattttcgtg aacgcatccg tatcaatggg
    10981 gtcttgtgtg aagaagcgtt tattacagat tttgtatcca gataccgacc ggtcattgaa
    11041 gagatcgaac ccagtttttt tgagatcacc gttgccatgg ccttcctgta ttttgctgaa
    11101 aaaggtgtgg atatagctgt tatagaaaca ggactcgggg gaagacttga ctccaccaat
    11161 gtcataactc ctgtgttggg tatcattacc aatatcggct gggatcatat ggccatgctg
    11221 gggaatacac ttccgcttat tgcagctgaa aaggctggta tcatcaaacc gggtgtgccg
    11281 gttctcattg gcgaaagaca ggaagaggtg caaggagtat ttaaggagat ggcagtctta
    11341 caaggtgctc cgttgtataa cagcgacaat cttgtacgcc tggaagttct ttccaacgat
    11401 cctgcaggtt tgtgctttcg ggcacaactg gagaccacac aattcacact ggaaacctgc
    11461 acccctatgt ttggcagcta ccaggaaaaa aacctgcaaa ccgttctggc cgctgctgat
    11521 ctgttgcagc aagactggca gatttccgta aacgatatca tttccggatt acaacaggtg
    11581 aaggaactga caggattcag agggcgcatg gagattctgc agcatcagcc gctggttctt
    11641 gctgattgtg cacacaacag tgacggatta gatcagctac gaaggagtat agatgccatg
    11701 gatgtaccgc actggcatat cgtaaccggt gctgtgcagg ataaagatgt agctgctaac
    11761 ctccttcatt tgcctgaaag tgccagctac tatttctgta aaccggatat tcccagagga
    11821 atggacgccc ggacattgca gcaacttgca gccaatatgg aacgaaaggg cgtggcatat
    11881 ggttcagtgc acgaagcaat agtcagtgca ctgtctaatt caggctccga tgaagctatt
    11941 ctgatttgcg gcagtatttt tgtggtagca gaagcgctgc cgaacttcaa acataagaca
    12001 tgaaaagact tctgatacct gtacttcttg cgctggtatt atctgttttg acacagcctg
    12061 tcatggctca gaagggttat gactaccagg taaccatacg tggaatggaa gataccgttc
    12121 tgtacttcgg acatcattac gggaataagc aatatgttat agatactgtc aggaccgata
    12181 agaatggtac tgcacatttt gccggccagg agcctctgcc aggaggcgtg tacattgtgg
    12241 tcatgccaaa aatgaacaac aagtactttg aaatgatcgt caatgaaccg gcatttgaga
    12301 tggctacaga tactacagac atcaccggac atatgcaggt gaccggttct gtggagaatc
    12361 aggtcatgta cgatgatctt cgttttattg gtgaccgtcg cagtcgggtg aatgtattgc
    12421 gaaaagaaca ggaagctgca gagcagggaa gtaaaagagc tgaaaagatc caggaagaaa
    12481 tggacctgat caatcaggaa gtactggact ggagaaacaa cctctatgca aagtatccgg
    12541 gtaccttcta tgttaagttg ttgaaggctt ccgcagatct taccctccct gaagcaccgg
    12601 tactgcctga tggaacgcct gataaaaagt atctgataca gtgggtgcat gagcattatt
    12661 tcgatgacat agatctgaaa gattccagat tgctcagaag cagtgtattg catatgaaag
    12721 tggaacagta tctgaacacc tatgtgccca agatacccga ttcagttagt gccgctattg
    12781 acaggatcat agaaggagcc cgcggtaaca atgaggtatt tcagtacctg gttgttaccc
    12841 ttttgaatca atatgcctcc agcgatatta tgggtatgga cgctgttttc gtacacatgg
    12901 ttgacaagta ctataaatca ggagatgctt tctggctgga tgatgctgcg ctctttcgta
    12961 tcacaaacaa ggcagcatta atgaaaccaa cactgatagg aaagcaggcc cctcccatta
    13021 tgctgcagga ttctgcaggt atagacattc cgctttatag tatagactca aaatacactg
    13081 tagtggtttt ttgggatgta gattgtggtc attgcaaaaa agaaatgccc aagctggtag
    13141 aagcctaccc gaaaataaaa gagcttggtg ctgaagtgta cgctgtttat acagaagaag
    13201 aatgggataa atggaaggat tggctggatg aaaaacacta caactggatc aacgttggaa
    13261 atgtgaaaag agaaagcaac tttcaggcct tatataacgt agatcagacc ccgctccttt
    13321 tcattctgga taagaataaa aagatcatcg ggaaacacct gaaagccgaa caattgtatg
    13381 atttcctgaa aaactaccag gagttcaatg acctataaac acctcaagat caataagcgt
    13441 gattcgttcc gccctgtgct gcaaaaatca gacaaggaat gggaactgga actgagtcag
    13501 gatcagtatc atatacttcg caacaagggc actgaacgac cgtttaccgg agctttcgat
    13561 gcgcactttg aaccaggcaa gtatgtatgt gcaggttgtg gcactcccct cttctatagt
    13621 ggggcaaaat tcaattctca ctgtggctgg ccctctttcg atgctcctgc aacagatgag
    13681 aacatcacct atcttaaaga taccagccat ggtatgatca ggatcgagat cgtgtgttcg
    13741 aattgtggcg ggcacctggg ccatgttttt gacgatggtc caacagatac cggactgcgc
    13801 tattgtgtca attctgcttc gctccttttc aagaaggagg aagattgatc agtcggcttt
    13861 gcgaagggtg aaagcgaaag ttgaaccctc atttggagta cttctgacgg ttatggtctg
    13921 gttgtgtgct tcaatcacat gtttgactat actcaacccc agacctgttc caccaaaatc
    13981 cctgcttcgg cttttttcca ccctgaaaaa tcgctcaaat acccggctta gatcttcttt
    14041 gccaattcct atcccggtat cagaagcttc caccagaata ttatcaccca tattatagaa
    14101 tcctacattg gtctggccat tgtcttttcc gtatttgatg gaattggtga tcaggttgac
    14161 cagaacctcc cggatcattt tcttatctgc ctctaccata aagacccgat cgcatccttc
    14221 tttgatcttt agcctgatgt ttctctgagc agcctggatc tccagatctg aaaacacttc
    14281 cttggtaagt gcattcatgt caaacggctg catctgtagt tttaactgcc cgctttccag
    14341 atgcgatatc gactccagat cattgatgat actaaccatg cgttccacat tccggatggc
    14401 cttgctcagg tagtccatat tgatacgcgg gtcattaatg cctccatcga tcaatgtttc
    14461 cagatacccc tggatactgg tgatgggggt tttcagttca tgcgacacat tgcccaggta
    14521 ctctttccgg tagttctcca tactgcgaag ctggctgatc tcttttctgg aagcctcagc
    14581 ccagttcaac acttctttat ttacagcccc gatgatatct gtgctgaggt caagtctgga
    14641 ttccaccaca ttcttggtgg tcttcagatt gtgtatattt ttataaataa gttttatctt
    14701 cctgtaaata aaattttcaa ccaccctgta aaaaatgagg taggtcaagc caaacatgag
    14761 tattggtaca agcagcagat ggtatctgaa atcattcagt acaggaagta aggaaggtat
    14821 cacttccagg accactacca gcagcaccac cagcagggtc gcccgtaaga cgatctgcct
    14881 gggcgaggga ttcttcatca ataatcaaat ttatacccta tacccttgat ggtcttgatg
    14941 tattcttcac cgatcttttc gcgtagttta cgaatgtgga catcaatggt acgatcgccc
    15001 acgatcacat ccatgcccca gatatcgttg aatatctcct gacgtgtaaa tacccgccca
    15061 ggtttggagg tcagcaattg cagcagttca aattccttct tggccaggtt gatgggttct
    15121 cctttgcgga ataccaggaa cttttcgcgg tcgatgatga gatcaccaaa ctgttgctga
    15181 ccggaattct gcagatctgc agcagttcga cgcagcaatg ctttaatgcg actgatcagc
    15241 agtcttggtt tgatcggttt ggtgatatag tcatctgcac ccacatcgaa accctgaacc
    15301 tgggtaaaat cttcattgcg ggcggtcaga aatgcaatga tggtgttgtc ataatcattg
    15361 ttgctgcgca actccctgca tacttcaatg ccatccattt ccggcatcat gatatccagc
    15421 aggatcagac ttggtttttc actggcggcc attttaagcg catccttccc gttgctggcc
    15481 ttcactacat catagtcttc tctggtcagg ttgtattcaa tgaactccaa tacatcgggt
    15541 tcatcatcta ctatcagtat cttgatcttg ctggacatac ctgaaatttt tgtaaaggta
    15601 gaaccacaac cttaaggacc tttgaattag gtgttaattc aagataaatt taataccgaa
    15661 ttaacaactg gactaataac agtacgattt gtaaaggatc atgtaatcta cgatattctg
    15721 gatggcagcc gttttcatgt cacgtggttc agcggtggct tgcaatgctt cactgcaaag
    15781 catccagacc tcacaccagt tggtgtccat agcttcctg
//
LOCUS       DAOMAX010000031        15794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_413_length_15794_cov_4.618146, whole genome shotgun sequence.
ACCESSION   DAOMAX010000031 DAOMAX010000000
VERSION     DAOMAX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15794
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_15794_cov_4.618146"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK742_03100"
     CDS             <1..712
                     /locus_tag="PK742_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75605.1"
                     /translation="RITRYAMENYKLGSSGIAPAITESAIDFSSLQQYYASFDPSRTG
                     GIQVFPGLLHDTIVSIIGVSVADTLKNASLRLLHPGGNKFNVLQHPISRDSAISLIEN
                     YFNGVRINGRVLNKHDDIDKISRYYPWSNLNTYFTDNGGNTTNQVDYHILLYHGWVEN
                     DTTLLNDLFNTGDTRNYIGSADSLVGYTGLWELFRSNRSVTESPTSPNTRKGLIEEIA
                     KPCPPRCDDLNGITKNDL"
     gene            807..1436
                     /locus_tag="PK742_03105"
     CDS             807..1436
                     /locus_tag="PK742_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75606.1"
                     /translation="MSGLLACLVICLLWKTSASADRRLMWMIASLYLMEIPAAGIVYF
                     NPHRPSNIWYYNLVLTGVYAAYISRYSVLFIEKKLAQTARVLALLFIVAHIMNMQFGQ
                     QMKMLCTNTLVPANIVLAILAFLHLREQVQRLTEHPLYQFSTWFSISTFVYFTGTVPI
                     IAVLNYWSAENIELADKLYIINDVLNVFYFLLIIAGLIWTKIKQTYSTS"
     gene            1400..2200
                     /locus_tag="PK742_03110"
     CDS             1400..2200
                     /locus_tag="PK742_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU75607.1"
                     /translation="MDKNQTDILYFLIITTSLLVFFSVLVINLLLVSRNRKLRHINEM
                     HRLQVLQEKELANVRLEVTETTLREVAADLHDDTGQLLTVAIMQLNRLSKNAPDDTVE
                     QALHAVRSGLDSVRNISRILNSDYLRSFGLKTALQRLKERLEKGGKLHLRYDLDDDLV
                     WSDPSLELHAFRIIQELVSNTIKHAEATEINIQLNMMEDKISMYYTDNGKGLPDDAGR
                     PAAPGAGLLNIEQRVKYMGGQIIPLQEAHRGFHLKFLFPTGLNKKEST"
     gene            2197..2853
                     /locus_tag="PK742_03115"
     CDS             2197..2853
                     /locus_tag="PK742_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU75608.1"
                     /translation="MTQRSVAIVDDHQLIRSGLRLLIEGFENYTILFEAENGSEALHY
                     LRRSESYPDVVLMDIGMPVMDGFETTAILTAEFPAIKVIALSVYDDDATISRMMESGA
                     HAYLLKDASTNEVKETLDEVERKGYSYSDRVINSIMQAKEHAIQQKTSRRPIDHLSER
                     ERTFITLCCSELTYKEIADKMEVSQRTVDGYRESVFSKLDLRSRTGIVLFAFHNGLVS
                     "
     gene            complement(2860..6198)
                     /locus_tag="PK742_03120"
     CDS             complement(2860..6198)
                     /locus_tag="PK742_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75609.1"
                     /translation="MSGNYTYLINRLDAFIRKFYLNQVIRGVLWLVAVAGSMYLVFSL
                     LEYYLYLSPAIRKLFFYGFLVISTALLAGGIILPLIRYFRLGKVMNYQTASSIIGSHF
                     PEVEDRLLNILQLEHQSPGVADRSLIEASVEQKIRQIKPVPFLRAIDLRKNKKYLPYA
                     LPPLAVLFFLLFAAPNILRDSNYRLINNNEVFERAAPFHFMISNDPLEVIQYQDFELV
                     VHLEGSVLPEAVAVETAAGSFPMTRTAPDTYTYVFRKVPDATDFHLLANGYPSSTYTL
                     RVIPRPVVKTFRVELDYPDYTGRRNEVLDNTGDMSIPEGTSVRWVFSTEAADSVELFF
                     GENKQTAQRDTRDRFVLEKTIHDGGSYAIGLANTYMYSNDTLRYWMQVIPDAYPKISV
                     EAATDSSDHKYVFFLGNASDDYGISRTAFHYTLEKQDEQGHYRESDTGTDKVPFTRAG
                     NQAAFTYAFDMNRFALQPGDRITYYFETWDNDGVHGPKATRTGVMQFAVPTLDELKDT
                     RDANNDAVEEGLEKVLDELQDIRKDTRDLQEKFIQKKDLNWEDKKNVEDLLKRQNQLQ
                     EQLNQLQENFQKNLDMQQEYMQPEQQILEKQQQLQELFEEVMDEEMKQLYEELQKLLE
                     ELSREESLQEMEQMDLNNKELENELDRMLELFKQLQLEQQMQETIDELNELAEEQLEL
                     SEESKDKQSDVMDNIDKQEDINKQFEEISKDLEKLQEMNEDLENPKEMGDSEQQQQEI
                     SEHLEQSMEQLQNNKQKQASEQQQQAGEKMQQMAQQMQDQMQQQQQEQQQEDMRALSQ
                     LLDNLLKLSLDQEDLIYELQQTKAANPRYVELMNEQQRIKEDTRMVEDSLLALSKRVP
                     DISSFVLREMAAVNKNLGGSLDALHERGTNQANVYQQYTMTGYNNLALMLDEVLQQLQ
                     QQMAQSMPGSQMCQKPGGSSELPSMSEMQKQLNEQLQQMKGQMSQGPKPGERQGMSQQ
                     LAQMAQQQAALREALQQLADELGGGNTEDGKLAKELREIADQMDKTEEDIVNKRLTEE
                     TLKRQEEILTRLLEAQDADRQRKTDNERQSNTADAQQREMPPALEEYLRKRQAELDQY
                     KTVAPELKPYYKQLVEEYFRNGGW"
     gene            6332..6856
                     /locus_tag="PK742_03125"
     CDS             6332..6856
                     /locus_tag="PK742_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75610.1"
                     /translation="MYENNMPDRRYRKTLSLMKTHLPAPARIMDLGVDNPFSEIMRKE
                     GYTVDNTGGEDLDEEVSLAKATGYDAVTAFEILEHLLSPYTLLKSIEAPKLIISVPLN
                     VWFSGPHRNPKDPRDNHYHEFYPYQMDWLLDKTGWKIDHSETWTSPDRWRPGIRPLLR
                     FIWPSYYFVVCSRK"
     gene            6922..8349
                     /locus_tag="PK742_03130"
     CDS             6922..8349
                     /locus_tag="PK742_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75611.1"
                     /translation="MSGLIGFFFLSAVAYAQSNYIGSGICLDFPEEPDNYVDLGDVYN
                     DLDFPLTFEAWVYPHTKASGNFYGLFSSDASGTGNYYGLWIRINEDDHVELEIGSGAG
                     AGTSHRRGLRTINTIPFETWTHVAIVATSATDMTFYFNGVEQSSVSTGGTAGTTSILH
                     NGNHAFIGRNINVHQNDQTFGQMDEIRLWDTERTAEEIRSHMCSKLTGGEAGLLGYWI
                     ADESFTATEVLDMAGSADGTIVGSVDRILSAAPLGNESYYLFSDVWGGVSLSIGSSGG
                     DLFEVTEVGSDVYGIFIYRVDGEPYADAGLLPHPAYYFGVFPLTTSTPEFGVRYNYSF
                     DNGVVSALNESSSDWSVHDDGADESWVTEGPELDTTLNQLSNTGNGRQEYIFGVSSPK
                     NAVSDMVIPLKGLWPNPASEKLHFNTVTGNGMVRIFNMSGEQMYVQSFQQAIDEVPVT
                     GLASGQYVLLIDADGKTSGALFVKE"
     gene            complement(8323..9474)
                     /locus_tag="PK742_03135"
     CDS             complement(8323..9474)
                     /locus_tag="PK742_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HQU75612.1"
                     /translation="MTAGSGRIVFLDVVRAFAILMMLQGHFVDTMLDPAYRDHSNWIY
                     YSWSFMRGITAPVFFFSSGLVFTYLLTRKAKPWRENERLYKGIGRGLQLIAIGYLLRI
                     NFLALLFKFDIYRWVAGVDVLHVIGIALLSLIGLYILHQEFRISLPWLLGITGLLVFY
                     LDPMVERGNYSLVPDFLSNYFVNQGYSTFTIFPWIGYTLLGGVVGNLLARYPKVAHTW
                     WLPVLLLGTGLLFHYQSIEALVFLYRNTGVSGFIEWRINNSHLLIRLGDVWVVMALII
                     WITRIWKNMPALIPKIGSETLTIYSVHYIVLWGTWFGLGINRLGSKTWDPWMSAMGAL
                     LFVISFIILIRYIDVIRNRWAAWVREPVSLYYRFVRKKVRLLFLYEKSA"
     gene            9661..11718
                     /locus_tag="PK742_03140"
     CDS             9661..11718
                     /locus_tag="PK742_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQU75613.1"
                     /translation="MPLSTKWLMLAQPFCWLWQIASGQTCDLLGADLSAQTDALVREE
                     MLKQHVVGLTLGIIRNGEVVYMKGYGFADREYSIPAEVSTIYRFASLSKVITSVAALH
                     AWENGLLNLQADVREYVPEYPAKPEGIITVEQLLACEGGIQQYNQVSDFNTDALVQYI
                     LDHPQDYDPVAAIDIFKDQALLARPGTQFNYSTFSFNLLAAVIERAVGMPYETYVDSL
                     LIQPMQLPYLQPEFRALRPYPNQSSWYRVEGDSAIADKIYGYDYEDVSWKLGGGGYTG
                     TIVDLSGFARALLNGSLLKKNTRNMMFTNRKVNGKNSWYGMGVFLGSRHGEPIVSQFG
                     HQAGARSIMYLAPESRDGILILSNTYGTDLKSLAEGLWDITRKSTRDTLCLDPFSDQL
                     TQPVWKGEPEVAGTGVTLHWDPIEQANHYEVQWNTRNEFGEVTMTDPTNWTRQLEMER
                     GLKQITSIPSIYLYDLPANTTVFLRVRALNDYLHEGVEGPWSKVLKFRSGSTSQAAPV
                     PYTMVFDKLNSIGQGDALLEASPGVEWKLVRSEDGLRWRSGRWSLAPSGGGLTLDVFP
                     GNGHASGFLELTMDLTGYSESTLRFSLDYLLHGTGDELPEVACWVRGDADDTWIILPE
                     KLNGNGQPGEQHTEFELMEILQNYGQQPGEFFQFRLGFYAGSDAHSYLPQPAITLLSC
                     VVE"
     gene            complement(11749..13764)
                     /locus_tag="PK742_03145"
     CDS             complement(11749..13764)
                     /locus_tag="PK742_03145"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQU75614.1"
                     /translation="MSDLQAFQKAILAGIPKELPPPAAYDPSVNHAPRRKDLLGPAEK
                     ELAVRNALRYFAPEHHAALAPEFADELRTYGRIYMYRFRPAYAMYARHLDHYPHQSRQ
                     AAAIMMMIQNNLDPVVAQHPHELITYGGNGAVFQNWAQYLLTMQYLATMTEEQTLVMY
                     SGHPMGLFPSHVDAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGSYMYIGP
                     QGIVHGTNITVLNAARMMDKNASDCRGKIFVTAGLGGMSGAQPKATVIAGGVCVVAEV
                     NPKATHTRHSQGWVDEVFDDLDALIPRMLQARENKEAVSIAYQGNVVDLWERLAKDDI
                     HIELGSDQTSLHNPFAGGYYPAGLSFEEANRMMADDPEAYKEKVYASLRRHVAAVNQL
                     VEKGMYFFDYGNAFLLESSRAGADILKEDGLFRYPSYVQDIMGPMCFDYGFGPFRWVC
                     ASNDPKDLETTDRIAAEVLEAYRNEATDDISLQLSDNINWIRSAQENKLVVGSQARIL
                     YADALGRIRIAQAFNEAIRKGEISAPVVLGRDHHDVSGTDSPYRETSNIYDGSAFTAD
                     MAIHNVIGDGFRGATWVSIHNGGGVGWGEVINGGFGMVLDGSDAASRRLESMLFWDVN
                     NGIARRSWARNAPAMTAIEREMQRSGNLKVTLPNLVDDAVLSDLFRD"
     gene            13982..14419
                     /locus_tag="PK742_03150"
     CDS             13982..14419
                     /locus_tag="PK742_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75615.1"
                     /translation="MRRYYPFLIVMIAGLGLMSCQDDTPYYDVRDEYIGDYDVADDCD
                     AFSSDYALTVFKEGNDNEIIFGFPGLYETGYEVVALVTGMKITIPIQQFVVATWPAEV
                     IYEFSGSGALDGDILTVDYQVLTIQNGLIYDDVDCIATMTRVY"
     gene            complement(14476..15702)
                     /locus_tag="PK742_03155"
     CDS             complement(14476..15702)
                     /locus_tag="PK742_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU75616.1"
                     /translation="MRFVLFMFILNLLFPASAQVFTQYTPDNSDVPDLLVHALHKGPD
                     GSIWICTESGLVKHDTGGIWTLFNTSNSGLPDNSVRSVYAESDGVVWVGTFLGGLARY
                     DGADWTVYNSSNSGLPENFIRCINRDLSDTMWIGTNAGLARWDGADEWTTFTIDNSGL
                     LSNNIVDIYVDENNDLYLGTLNGGLSTYIDGVIDYYRTENSFIADNTVMAVAEDAFAN
                     KWMATAFGGLSIFTEAGDFLNFNPFNSDIPDLEVNDVALDNEGLGILTMAATGLVQFD
                     GSNWVIWDTENSAIPTNYLNSVVVDDANRIWIATEDAGLLVLERNAVGVDVLSSAENT
                     EIYPNPAHDIVFLHTADGASLNWQLYNNAGMLCRSGMTRQNTTQILLNDLPAGLYHIR
                     VFHQGQPAGTSYLIIE"
BASE COUNT         3889 a         3910 c         3932 g         4063 t
ORIGIN      
        1 caggatcacc cggtacgcaa tggaaaatta taagctgggt agttcaggta tagcccccgc
       61 cattacagag tcagcaatag acttcagtag tttacagcag tactatgcca gctttgaccc
      121 ttcccgcact ggcggcatac aggtatttcc tggtttattg catgatacga tagtctctat
      181 tatcggtgtt tcagtggcag ataccctgaa gaatgcatca ctaaggctgc ttcatcccgg
      241 aggcaataag tttaatgtgc ttcagcatcc catctccaga gattcagcga tcagtcttat
      301 tgaaaattat ttcaacgggg ttaggataaa tggcagggtt cttaataaac acgatgacat
      361 agataaaata tcccgctact atccgtggag caacctgaat acctatttta cagataacgg
      421 tggaaatact acgaaccagg ttgattatca tatcttatta taccacggtt gggtggagaa
      481 cgatacgacc cttcttaatg atctgttcaa cacaggggat acacgcaact acattggcag
      541 tgcagattcg cttgttggat ataccggact gtgggagttg tttcgttcaa atagaagtgt
      601 tacagaatcg ccaacatcac caaatacaag aaaaggacta atagaggaga tcgccaagcc
      661 ttgtccgccc agatgtgatg atctgaacgg cattacaaaa aacgatcttt aatagtacct
      721 tgtatgcctc atactgacag ccattggaac attcgggcaa actattggag tttttatgga
      781 tagtacacgc acatcaggtc gtgtatatgt ccggactgct ggcctgcctg gtcatttgtc
      841 tgttgtggaa gacttctgcg tctgcagacc ggcgtcttat gtggatgatc gccagcctgt
      901 acctgatgga aataccggct gcagggatag tctatttcaa tccacacagg ccatccaaca
      961 tatggtacta caaccttgtt ttgacggggg tgtatgctgc ctacatttcc cggtattcag
     1021 tgctattcat cgaaaagaag cttgcccaaa cggcccgggt gctggcactg cttttcatag
     1081 tggcgcatat catgaatatg cagtttggac agcagatgaa aatgctttgt accaatacgc
     1141 ttgttccggc aaatatcgtg ctggccatat tggccttcct gcacctgaga gaacaggtac
     1201 agagattgac agaacatccg ctctatcagt tcagcacctg gttttcgatc tccacattcg
     1261 tgtattttac cggcaccgtt ccgatcattg ccgtattgaa ttactggagt gctgagaata
     1321 tagaacttgc agataaattg tatattatta atgacgtttt aaatgttttt tattttctat
     1381 tgatcattgc agggcttata tggacaaaaa tcaaacagac atactctact tcctgatcat
     1441 caccacatcc ctgctggtat ttttttcggt gctggtcatc aatttgttgc ttgtttcgag
     1501 aaacaggaag ctgcgacata tcaatgagat gcaccggctt caggttctgc aggagaaaga
     1561 attagcgaat gtcagactgg aagtgacaga aactacttta cgggaagtgg cagcagatct
     1621 tcatgatgat acagggcaac tgctcaccgt ggccattatg caactgaaca ggctttcaaa
     1681 gaacgcacct gatgatacgg tagaacaagc actgcatgcg gtacggtctg gcctggacag
     1741 tgtgcggaac atcagcagga tactgaacag tgattacctg aggtcattcg gattaaaaac
     1801 agcgctgcaa cgcttgaaag aacgcctgga gaaaggtggt aagctgcacc tgcggtatga
     1861 tctggatgat gaccttgtat ggtcagaccc ttcgttggag ttacacgcat tcaggatcat
     1921 ccaggagctt gtgtccaaca ccatcaaaca tgcagaagca acagaaatca atattcaatt
     1981 gaatatgatg gaagataaga tcagcatgta ctatactgac aacggaaaag ggttgcctga
     2041 tgatgcgggt cggccagcag ccccgggtgc aggtttgctg aatatcgagc agcgtgtgaa
     2101 gtatatgggc ggacaaatca tcccgctgca ggaagcacac aggggttttc atctgaagtt
     2161 ccttttcccc accgggctca acaaaaaaga atcaacatga cacaacgatc tgtcgccata
     2221 gtagatgacc accagctgat caggtcgggc ctgcgattgc ttatagaagg ctttgaaaat
     2281 tataccatcc tgtttgaggc tgaaaacggt tcagaagcat tgcattatct gcgcagatca
     2341 gaaagttacc cggatgttgt tttgatggat ataggcatgc ccgtcatgga cggatttgag
     2401 acaacagcga tccttactgc agaatttcct gccataaaag tgatcgcttt atcggtctat
     2461 gacgatgatg caacgatcag caggatgatg gaaagtggag cacacgctta tctgcttaaa
     2521 gatgcatcaa ccaatgaagt aaaggaaact ctagatgagg tggaacgaaa gggatatagt
     2581 tattcagacc gcgtgatcaa cagcatcatg caggcaaagg aacacgcaat ccagcagaaa
     2641 acctcgcgtc gtccgataga ccatctaagc gaaagagagc gaacctttat aacactctgc
     2701 tgcagtgaat taacatataa agagattgct gacaaaatgg aggtaagcca acgtaccgtt
     2761 gatggatacc gggaatctgt tttttccaaa ctggatctga gatcaagaac cgggatcgtg
     2821 ctttttgcct ttcataacgg gctggttagt taatacacat caccacccgc cgttccggaa
     2881 atattcttct accaactgct tgtaataagg cttgagttcc ggggcgacgg tcttgtactg
     2941 gtccagttcg gcctggcgtt tgcgcagata ctcttccaaa gccggcggca tttctctctg
     3001 ttgtgcgtct gcagtattgc tctggcgctc attgtctgtc ttgcgctgcc gatcagcgtc
     3061 ctgtgcttcc agcaatcggg tcaggatctc ttcctgccgt ttcagggtct cttctgtgag
     3121 gcgtttgttg acgatgtctt cttcggtctt gtccatctga tcagcgatct cccgaagttc
     3181 tttggccagt ttgccgtctt cggtattgcc accacccaac tcgtcggcca gttgttgcag
     3241 ggcttccctt aaggcagctt gttgctgggc catttgtgcc aactgctgac tcataccctg
     3301 gcgctcaccg ggtttcggac cctggctcat ctgacctttc atctgctgca actgctcatt
     3361 gagctgtttt tgcatctcgc tcatactggg aagctctgat gacccgcctg gtttctggca
     3421 catctgtgag ccgggcatac tctgggccat ctgctgctgc aattgctgta atacctcatc
     3481 cagcatcagg gccaggttat tgtagccggt catggtgtac tgctgataga cattcgcctg
     3541 gttggtgccc cgttcgtgca gggcatccag actgccgccc agattcttgt tgacagcagc
     3601 catttcacgc agtacgaaac tgctaatatc cggtactcgt ttgcttagtg ccagcaggct
     3661 gtcttccacc atccgggtat cttccttgat gcgctgctgc tcattcatca attccacata
     3721 ccgcggattg gctgctttgg tttgctgtaa ttcatatatg agatcttcct gatccaatga
     3781 aagcttcagc aggttgtcca gtaactgcga tagtgcccgc atgtcttcct gctgctgttc
     3841 ctgttgctgc tgctgcatct ggtcctgcat ctgctgggcc atctgctgca tcttttctcc
     3901 tgcctgttgc tgctgctcgc tggcctgttt ttgtttgttg ttctgaagct gctccatgct
     3961 ctgctccagg tgttcgctga tctcctgttg ttgttgttcg gaatcgccca tttctttggg
     4021 attttccaga tcttcattca tttcctgcag cttctccagg tctttgctga tctcttcgaa
     4081 ctgcttgttg atgtcttcct gcttgtctat gttatccatg acatcagact gcttgtcttt
     4141 agattcttca ctcagctcca gctgctcttc agccagttca ttcaactcat ctatggtttc
     4201 ctgcatctgc tgctccaact gcagttgctt gaacaattcc agcatccggt ccagttcgtt
     4261 ctccagctct ttgttattca gatccatctg ctccatttcc tgcagagatt cttcccggct
     4321 cagttcttcc aaaagctttt gcaattcttc atacaattgc ttcatctcct catccatgac
     4381 ctcttcgaac aactcctgaa gttgttgctg tttttccagg atctgttgtt cgggctgcat
     4441 atactcctgt tgcatgtcca ggttcttctg aaaattttcc tgcaactggt tgagttgttc
     4501 ctgcaactga ttctgacgtt tcagcaggtc ctctacgttc tttttatctt cccagttcag
     4561 gtctttcttc tgaatgaatt tttcctgcag atcacgggtg tcttttctga tatcctgtaa
     4621 ctcgtccagc accttttcca gaccttcttc tacagcatca ttgttggcat cacgggtgtc
     4681 tttcagctca tccagggtgg gcacggcgaa ctgcatgacg cctgtcctcg ttgccttcgg
     4741 accgtgtacg ccatcattgt cccaggtttc aaagtaatag gtaatgcgat cgccgggctg
     4801 aagggcaaaa cgattcatgt caaaggcata ggtaaaggcc gcctggttgc cggcacgggt
     4861 gaatggcact ttgtctgtgc ctgtatccga ttcccggtag tgtccttgtt cgtcctgttt
     4921 ttccagggta tagtgaaatg ccgtgcgact gataccataa tcatcgctgg catttccaag
     4981 aaaaaaaaca tatttatgat cactgctatc tgtggctgct tccactgaga tcttaggata
     5041 ggcatcgggg atgacctgca tccagtagcg aagtgtgtcg ttgctgtaca tgtaggtatt
     5101 tgccaaaccg atggcataag atccgccgtc gtgaatggtc ttttccagta caaagcgatc
     5161 gcgggtatct ctttgtgcgg tctgtttgtt ttcgccaaag aagagttcca cactatcagc
     5221 ggcttccgtg ctgaacaccc accttacaga tgtaccttca gggatactca tgtcgcccgt
     5281 attgtccagc acttcattac gcctgccagt ataatcaggg tagtccagtt ctacccgaaa
     5341 cgtctttact accggtctcg gaatgacacg cagggtatag gtggaagatg gatacccatt
     5401 ggccagcaaa tgaaaatctg tggcatcagg tacctttctg aacacatagg tataagtatc
     5461 gggtgcggtc ctggtcatgg gaaagcttcc cgctgcggtc tctactgcca cagcctccgg
     5521 caagacactg ccctccagat gaacaaccag ctcaaagtcc tggtactgga tcacctccag
     5581 tggatcattg ctgatcatga aatgaaatgg cgctgcccgc tcaaatactt cgttgttgtt
     5641 gatcaggcga tagttgctgt cgcgaaggat attgggtgcg gcaaacaaca ggaagaacaa
     5701 cactgccagc ggcggaaggg cataaggcag gtatttttta ttctttcgca ggtcgatggc
     5761 ccgcaggaaa ggaacgggtt tgatctgtct gatcttttgt tccacgctgg cttcgatcag
     5821 acttctgtct gcaactcccg gcgactgatg ttccaactgc aggatattga gaagccggtc
     5881 ttccacttca ggaaaatgac tgccgatgat ggaagaagcg gtctggtagt tcatgacctt
     5941 gcctagcctg aaataccgga tcagcggcag aatgatccca cccgctaaaa gtgcggtgct
     6001 gatcaccagg aaaccataga agaataactt tcttatcgcg ggcgacagat agagatagta
     6061 ttccagtaaa ctgaaaacga ggtacatgga tcccgctaca gccaccagcc acaatacacc
     6121 tctgatcacc tgattcaggt aaaacttgcg gatgaaggca tccagcctgt tgatgagata
     6181 agtgtagttt ccggacatac tatccttgac aaacttaacg caaagcctgt tcctgttttg
     6241 tttgtgtaac gaaatttaac gctacaggtt ttgaaaggat gcaaaaagac cgtcgtttgc
     6301 acaaagattt atcttagtgc caaattctgt gatgtacgag aacaatatgc cggacaggcg
     6361 ctaccgcaag accctgtcgt tgatgaagac ccacctgccg gcaccggcca ggatcatgga
     6421 cctgggggtg gacaatccat tcagtgagat catgcggaaa gaaggataca ccgttgacaa
     6481 tacgggtggc gaagacctgg atgaagaggt cagtctggca aaggcaacag gctatgatgc
     6541 tgttacggca ttcgagatcc tggaacatct gctgtcaccc tataccttac taaagtcgat
     6601 agaagctccg aaactcatca tctcagtacc cctgaatgtg tggttttcag gtccgcacag
     6661 aaaccccaaa gacccgagag acaatcacta ccatgagttt tatccctatc aaatggactg
     6721 gttgttggat aagaccggct ggaagataga tcacagcgaa acctggacct cgcccgacag
     6781 gtggcgacct ggcatacgtc cgttactgcg cttcatctgg cccagttatt attttgtggt
     6841 gtgcagcaga aaatagcata ccggcatacc tgagcttgag ctgcaacttg gtaactttaa
     6901 gtgatgcagc taataattaa aatgtctggt ctgattggat ttttcttcct gtcagcagtt
     6961 gcctatgctc agtccaacta catcggatcg ggcatctgcc tggactttcc ggaagaacct
     7021 gacaactatg ttgatctggg tgatgtctat aacgaccttg attttccgct gaccttcgag
     7081 gcctgggtgt acccgcatac caaggcaagc ggcaactttt acgggttgtt ttccagtgat
     7141 gccagcggaa caggaaatta ttatggattg tggatccgca tcaatgaaga tgatcatgta
     7201 gaactggaga tcggcagtgg tgcaggtgcg ggtacctcac accgtcgcgg actgcgaacg
     7261 atcaatacca taccatttga aacctggacc catgtggcca tcgtagccac ttcagcaaca
     7321 gatatgacct tttatttcaa tggagtggaa caatcaagtg tttctactgg tggaacagca
     7381 ggcaccacat ctatcctgca caatggaaac catgctttca ttggccggaa catcaacgta
     7441 caccagaatg atcagacctt tggtcagatg gatgagatac ggttgtggga tacggaaaga
     7501 actgcagaag agatccgaag ccacatgtgc agcaaattaa caggagggga agcaggattg
     7561 ctgggttatt ggatcgccga tgaatctttc actgcaaccg aggtattgga catggcgggt
     7621 tctgctgatg gaaccatcgt tggaagtgtg gatcgcatcc tttctgcagc accattaggc
     7681 aacgaaagtt attatctttt ttcagatgta tggggaggtg tctcactcag catcggatct
     7741 tctggaggtg atttattcga ggtaaccgaa gtagggtcag atgtgtatgg catattcatc
     7801 taccgggttg acggtgagcc atatgcagat gctggtttgt tgccacatcc tgcatattac
     7861 ttcggtgtgt ttccactcac tacctcaacg ccggagttcg gggtgcgata caactactca
     7921 tttgataacg gcgtggtcag tgccttgaac gaatcatctt cagactggtc tgtacatgat
     7981 gatggcgctg atgaaagctg ggtgacagaa ggtccagaac tggatacgac actgaaccag
     8041 ctaagcaaca ccggaaatgg caggcaggaa tatattttcg gggtctcttc cccaaagaat
     8101 gctgtatcag atatggttat accactaaaa ggattgtggc ccaaccccgc ttcagaaaaa
     8161 ctgcacttta atacggtaac cggtaatggt atggtccgga tatttaatat gtccggagaa
     8221 cagatgtatg tacaatcctt tcagcaagcc attgacgaag tgccggtgac gggtcttgcc
     8281 tccggacaat atgtgctgct cattgatgca gatggaaaaa cctcaggcgc tcttttcgta
     8341 aaggaatagt aagcgcactt tcttccgcac gaaacggtag tacagagaga ccggctcgcg
     8401 aacccaggca gcccagcggt tgcggatgac gtcgatatac cggatcagga tgatgaaact
     8461 gatcacaaac agcagggcac ccatagcact catccaggga tcccaggtct tactaccgag
     8521 gcgattgata cccaggccga accatgtgcc ccataacacg atataatgta cgctgtagat
     8581 cgtcagcgtt tctgatccga tcttgggaat gagcgctggc atgttcttcc agatgcgggt
     8641 gatccagatg atcagggcca tgaccaccca gacatctccc agtctgatca gcaggtggct
     8701 gttgttgatg cgccactcga tgaaaccact gacaccggta ttgcgataca ggaaaacgag
     8761 cgcttcaatg gactgatagt gaaacaaaag tccggtaccc agcaggagta caggcagcca
     8821 ccaggtatgc gccactttgg ggtatcgtgc cagcagatta ccgaccactc cccccaataa
     8881 ggtgtatcct atccagggga agatggtgaa ggtgctgtaa ccctggttga caaaatagtt
     8941 cgacagaaaa tcgggtacca ggctgtagtt accccgctcc accatcggat cgagatagaa
     9001 taccagcagt ccggtgatac ccagtaacca gggtaaactg atacggaatt cctgatgcag
     9061 gatatacaat ccgatcagcg aaagcagagc gataccgatc acatgcagca catccacacc
     9121 cgcaacccag cggtagatgt caaatttgaa tagcagggcc aggaaattga tccgcagcag
     9181 gtaaccaatg gcaatcagct gcaggccgcg tccgatacct ttgtataatc tttcattctc
     9241 ccgccagggc tttgcttttc ttgtcagcag ataggtgaac accagaccgg aactgaaaaa
     9301 gaataccggt gctgtgatac cacgcatgaa tgaccagctg taatagatcc agttggaatg
     9361 atcgcggtat gcaggatcca gcatggtatc cacgaaatga ccctgcagca tcatgaggat
     9421 ggcaaaagcc cgcaccacgt ccaggaaaac gatcctgccg gaacctgctg tcaagttatt
     9481 cgttttgata ctaatgtatt aagcggtctg tctgaagcga gtagatgttg atgctgtcac
     9541 cgcttttcgg ccccccggaa caccccccaa tcagggtgca aaggtacgat atttgcaaca
     9601 tgtataacat cttcccgtac ttactggaac gtaaaaggaa tcagataagt ttgtagcccc
     9661 atgcccctgt caaccaaatg gcttatgctg gcccaaccct tctgctggct ctggcagatc
     9721 gccagcggac aaacatgtga tctgctgggc gctgatctgt cagcccaaac cgatgccctg
     9781 gtgcgggaag aaatgctgaa gcagcatgtg gtgggcctta cactgggtat tatccgcaac
     9841 ggcgaagtag tgtatatgaa aggctatggg tttgccgaca gagaatacag cattccagcc
     9901 gaggtatcta ccatctaccg ctttgcttct ttatccaagg tcatcaccag cgtggcagcc
     9961 ttgcatgcct gggaaaacgg gctcctgaat ctccaggcag atgtgcgtga atatgtgccg
    10021 gaatatcctg caaaaccgga ggggatcatc accgtggaac aactgctggc ctgcgaagga
    10081 ggtatccagc agtacaacca ggtctctgac ttcaatacgg atgccctggt acaatatatt
    10141 ctcgatcatc cgcaggatta tgatcctgta gcagctattg atatcttcaa agaccaggcg
    10201 ctgctggcca gaccaggcac ccagttcaac tattctactt tcagtttcaa cctgctggca
    10261 gctgtcatag aaagagctgt cggcatgccc tatgagacct atgtggacag cctgctgatc
    10321 caaccgatgc agttgcccta tctgcaaccc gaattccgtg ccctgcgtcc gtatccgaac
    10381 cagtcatcct ggtaccgggt ggaaggtgat tctgccattg ccgacaagat ctatggatac
    10441 gactacgaag acgtttcctg gaaactgggt ggcggtggtt ataccggtac catcgtagat
    10501 ctgtcgggtt ttgcaagagc tctgctaaat ggcagcctgc tgaaaaaaaa tacccgcaac
    10561 atgatgttca ccaaccggaa ggtgaacggt aagaattcct ggtatggcat gggtgtattc
    10621 ctcggtagcc ggcatggaga acctatcgtc tcacagttcg gtcatcaggc aggtgcaaga
    10681 tccatcatgt atctggcgcc ggaatccaga gacggcatcc tgatactcag caatacctat
    10741 ggaacagacc ttaagtctct ggctgaagga ttgtgggaca tcaccagaaa gtccacccgc
    10801 gatacgctct gcctggatcc tttttctgat cagctgacac aaccggtatg gaaaggagaa
    10861 cctgaggtgg cgggtaccgg cgtcacgctt cattgggatc ctattgaaca ggccaaccac
    10921 tacgaggtac aatggaatac caggaatgaa tttggagagg tcactatgac cgacccgacc
    10981 aactggacca ggcaactgga gatggaaagg ggtctcaaac agatcacctc catcccatcg
    11041 atctacctct atgatctgcc tgctaacact actgtctttc tgagggtaag agcactgaat
    11101 gactacctgc atgaaggggt ggagggccca tggtcaaagg tgctgaagtt cagatccggt
    11161 tccacctccc aggccgctcc tgtgccttat accatggtct ttgataaatt gaacagcatt
    11221 ggtcagggtg atgctttact cgaagccagt ccgggagtgg aatggaaact agtgcggagt
    11281 gaggatggtt tgcgctggcg aagcggacga tggtctctgg caccatcggg cggcgggctt
    11341 accctcgatg tgtttcccgg caacggacat gcctccggat ttctggaact gacgatggac
    11401 ctcacaggct attcagaatc tacccttcgg ttcagcctgg attatctgct gcatggaacc
    11461 ggcgatgaat taccggaagt agcatgctgg gttcgtggag atgccgatga cacctggatc
    11521 atcctgcccg aaaaactgaa tggaaatggt cagcccggcg aacagcatac ggaattcgag
    11581 ctgatggaaa tacttcagaa ctacggccag caaccgggcg aattcttcca gtttcgtctt
    11641 ggattttatg ctggtagtga tgcccacagc tatctgccgc aacccgctat cacgctgttg
    11701 agttgcgtag tggagtaagg gataaagaag agttcaaggt gtgagggatc aatccctgaa
    11761 cagatcagac aaaacagcat catccaccag attaggcagg gtgaccttca ggttgccgct
    11821 tcgctgcatc tctctttcaa tagcggtcat ggccggcgca ttgcgtgccc agcttcttcg
    11881 ggcgatgccg ttgttgacat cccagaacaa catgctttcc agccttctgc ttgcagcatc
    11941 gctgccatcc agcaccatgc cgaatccacc attgatgacc tcaccccaac ctacaccacc
    12001 gccgttgtga atgcttaccc aggtggcacc gcggaaacca tcgccaatga cattatgtat
    12061 ggccatgtct gcagtaaacg cagagccgtc gtaaatattc gaagtttctc tatacggaga
    12121 gtctgttccg ctcacatcat ggtggtctct tcccagcaca acaggtgctg agatctcacc
    12181 ttttctgatc gcttcattga atgcctgcgc tatgcgtatg cgacccagcg catctgcata
    12241 caggatgcgg gcctgcgaac cgaccaccag tttattttcc tgtgcagaac gtatccagtt
    12301 gatgttatcg ctcagctgca ggctgatgtc atctgtggct tcattccggt aggcttccag
    12361 cacctcagct gctatgcggt ctgtggtttc gaggtctttc ggatcattgg aagcgcacac
    12421 ccagcggaag ggaccgaaac cataatcgaa acacatgggc cccatgatgt cctgcacata
    12481 actcggatat ctgaacaagc cgtcttcttt cagaatatcg gctccggcac gggatgactc
    12541 cagcagaaaa gcgttgccgt aatcgaagaa gtacatgcct ttttctacca gctgattgac
    12601 tgctgccaca tggcgccgta aactggcata taccttttct ttatatgctt cgggatcgtc
    12661 ggccatcatg cggttggctt cttcgaagct cagtcccgcg ggatagtaac cacccgcaaa
    12721 cggattgtgc agagaggtct ggtcgctgcc cagttctatg tggatgtcat cctttgcaag
    12781 tctttcccag aggtccacca cattgccctg ataggcgatg gacacggcct ccttgttttc
    12841 tctggcttgc agcatccggg gaatgagtgc atccagatcg tcgaagacct catccaccca
    12901 gccttgcgaa tgtcttgtat gggttgcctt cgggttgacc tcggccacca cacacacgcc
    12961 gcctgctatc accgtggctt ttggctgtgc gccactcatg ccacccagcc ccgcggtcac
    13021 gaagatcttt ccgcgacaat cggaggcatt cttgtccatc atacgtgctg cgttgagcac
    13081 agtgatgttg gtgccgtgca cgatgccctg cgggccgata tacatgtaag acccggcggt
    13141 catctgaccg tattgcgtca cgccaagtgc attgaatttt tcccagtcgt cgggtttgga
    13201 atagttgggt atcatcatgc cgttggtcac caccacccgc ggggcatcga catgcgaagg
    13261 gaacagtccc atcgggtgac cactgtacat cacaagtgtt tgttcttctg tcatggtggc
    13321 caggtactgc atggtgagca ggtactgcgc ccagttctgg aagaccgctc cgttgccgcc
    13381 ataggtgatg agttcatgcg gatgctgtgc taccaccgga tccaggttgt tctgtatcat
    13441 catcatgatg gccgcagcct gcctgctttg atggggataa tggtccaggt gacgggcata
    13501 catagcatag gccggacgaa accggtacat atagattcgt ccgtaagtgc gtagttcatc
    13561 agcaaattca ggtgccagtg cagcatggtg ttccggtgcg aagtagcgca gggcattgcg
    13621 aactgccagt tctttttcag ccggacccag caggtccttt cgtctcggtg cgtggttgac
    13681 tgaaggatcg taggcagcag gagggggaag ttctttagga ataccggcca gaatggcctt
    13741 ttgaaaggct tgcagatcag acatagagca aaggtagtga gtctctattg gtatcaggta
    13801 tcgggtattt tcataggtgt gggtttaaac ccgcacctat gaaagatgaa aaacatatca
    13861 cagattttaa cccacaccta tgaacgaaag acaaatacca tccaccgtta ccaagtgtta
    13921 actttgtaca actcagaaag actggcacag tgtttggctt tctattgtaa aatggacaac
    13981 catgagacgt tattaccctt ttttgatcgt gatgatagcc ggcctcggac tgatgtcctg
    14041 ccaggatgat accccttatt atgatgtgcg ggatgaatac atcggcgatt atgatgtagc
    14101 ggatgattgc gatgctttca gctctgacta tgcgctgacg gtcttcaagg aaggcaatga
    14161 caacgagatc atcttcggtt ttcccggctt atatgaaacc ggttacgaag tagtggccct
    14221 ggtaacgggt atgaagatca ccatacctat tcaacagttt gtggtggcca cctggcctgc
    14281 agaggtcatc tatgaattca gcggaagtgg tgcgctggac ggcgacatcc tgaccgttga
    14341 ctaccaggtg ctgaccatac agaacggcct gatctatgac gatgtggatt gcatcgctac
    14401 gatgacacgg gtgtattgac gaaaaacgtt tttcgtggtt cgttgatcgt tattcgtttg
    14461 gtctgattgt acttgttact cgatgatcag atagctggtg cctgcaggtt gcccctgatg
    14521 gaatacacgg atgtggtata agccggctgg gagatcgttc agcaaaattt gtgtggtgtt
    14581 ctgacgggtc attccgcttc tgcacaacat gcccgcgttg ttatatagtt gccagttaag
    14641 cgatgcacca tcggccgtat gcaggaacac gatatcatgg gctggattgg gatatatttc
    14701 agtgttttct gcagacgata aaacatccac ccctactgca ttcctttcca gcaccaataa
    14761 tccggcatct tctgtggcga tccagatgcg gttggcatca tccaccacca cactgttgag
    14821 gtagttggtg ggaatagcgc tgttttctgt atcccagatg acccagttgc tgccgtcaaa
    14881 ctggaccaat ccggtagctg ccatagtcag gatgccaagg ccttcattgt cgagggccac
    14941 atcattgacc tcgagatcgg gaatgtctga gttgaaaggg ttgaagttga gaaagtcgcc
    15001 ggcttcggtg aagatgctca gtcccccgaa agctgttgcc atccatttat tggcaaacgc
    15061 atcttccgca acggccatca cggtattatc ggcaataaaa gaattctctg tacggtaata
    15121 atcgatcact ccgtcaatgt aggtactgag tccgccgttg agggtgccca gatagaggtc
    15181 gttgttctca tccacataaa tatctacgat attattagac agcaatccgg aattgtcgat
    15241 ggtaaaggtg gtccattcgt cggcgccatc ccaacgggcc agacctgcat tcgtgccgat
    15301 ccacatagta tcagaaaggt cacggttgat gcaacggatg aagttctcag gcagccctga
    15361 attcgaggaa ttatagaccg tccagtcggc accatcatag cgtgccagtc cgcccagaaa
    15421 ggtacccacc cagaccacac catcgctttc tgcatacaca ctgcgcacac tattgtctgg
    15481 cagccctgag ttggaagtgt tgaacagggt ccagatgccg cctgtatcat gcttcaccag
    15541 cccgctttcc gtgcagatcc agatgctgcc atcaggtcct ttatgcagcg catgcaccag
    15601 cagatcgggc acatcagagt tatcaggagt gtattgggtg aagacctgag ctgacgcagg
    15661 aaacagcagg ttcaaaatga acatgaacag gacgaaacgc atggaataaa ggtagggaag
    15721 acacccgaca ggaaccaaaa aaaagacccg ctttcgcggg cctttctatc agataagaac
    15781 cattataaac tgtc
//
LOCUS       DAOMAX010000032        15725 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_417_length_15725_cov_4.449075, whole genome shotgun sequence.
ACCESSION   DAOMAX010000032 DAOMAX010000000
VERSION     DAOMAX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_417_length_15725_cov_4.449075"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="PK742_03160"
     CDS             <1..2115
                     /locus_tag="PK742_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75617.1"
                     /translation="DTKAAYLIELTVTDDLGCITSYKDTVIVTQPVADFVLGDTVPCL
                     DEIVVFEELATGSGITYLWDFGDGTTSTEANPEHSYGSYGAFDVTLIVTDDNGCSDTT
                     TKIAYVNVDSILGDITYDELVSSCNYSLVQFYVTTDDTICSYDWDFGDGFGGSGTSSP
                     IYPYLEAGIYDVSVTLTTCSGCVGTLTEEDFILVPGPYGSLEPLEDTVCIGNDAIFQL
                     NFSSTDTATIFYGNGDFGELDLPYQDELDSMFLDYAYDTSGIYVVSILLVDTNGCFNV
                     LDLPDSLWVGFPPNSIYAVDSGGCLGTEFFFDEGSTGLDPIVYWSWDIGDSVFVDTSG
                     ADFYYTYTEAGIYNTSLIVSTALGCADTLEFTVGVLDTPTVNISPDTVICPGNFVTLS
                     AEDGIVYSWSPPDGLDDPTSDTPSASPDATTTYSVVVSNGYCEASDTVQVRVLEELIT
                     NAGPDTALCLLGGVQLYGELVSDVPPDQIEFTWFPPTELSATDILDPYSSSADDITYY
                     LIASCGTLSDTADAQIQVVAPPDVEIAVDSILLIQDEVAQINASLLSSSSAVTYNWFP
                     PDQINCNNCSSVFVSPDVTTVYSVEVIDENGCTDIDFVYVRVMPCDETLMFIPNIISP
                     NNDGMNDIFRITYEGITLIEHIYVYDRWGELVAESKDPNKIWDGTYKGELCNPGVYVY
                     VIEAICVNDAPNIITGNITLVR"
     gene            2125..3126
                     /locus_tag="PK742_03165"
     CDS             2125..3126
                     /locus_tag="PK742_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU75618.1"
                     /translation="MNKVRSVILLCMLWVVSCKVQAQDWHYSTFHSAPTMINPAFTGL
                     FNGDLRIIGNYRSQWQFANAYETFSMAADGAFAVINNGDFASAGLTILADQAGDLNFT
                     TMHTAASMAYSKALNGYGNQYLTLGLQAAVNRRSIDYTNMVAFDPEPFATDVQDHYSY
                     LDMTVGASWYFLPSDEIYIFLGGAWFHFNEPNQSFLNNSEDILESRYSLHGGMQFPVM
                     DRMNILPSVMYNMQGQYTEFLLGSYLKFKLNHVTVNDETALYAGVWYRTSDAAVVSVR
                     YDYNTTVFALSYDVNTSDLSSASYGRGGLELNVIYVLDKKFSISKRLKNRSYSCPKF"
     gene            complement(3123..4658)
                     /locus_tag="PK742_03170"
     CDS             complement(3123..4658)
                     /locus_tag="PK742_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75619.1"
                     /translation="MNRKLIIICCTLLTYVLLVVSWGYQFGRGDLVQLDPLMVHAAHP
                     ELYPNDLYVQEAESTFPNERFFFLLLLRPFTGHLEWVSFLYHVFFSLLLLMGLYRLSS
                     RYLHSTWLRLAVPLIVFIPLYGINLGQNELYYGIFHPSLVAKSIGIWSIVFWLRRRVI
                     LALSVAALSTLFHPVAGLQVFSLVVAGEALWSITDKKSWLPKRLFIGILIWFCTAGLF
                     LILVHRRFETGVVPEGMDLYQIFYVFRNPHHYLPAAFGQRNWVILTPLIILGSIIWFF
                     RESRLGWFFVAAFISLGIYCAGVQMRSETITTLQWFKSTIWLEMLSVIALLHMAENIL
                     DRMPATLHKAALPVILLLPVLWGALISPGNIYRQATPYYDLPFRDYTDDAIDISRQAK
                     ALTPTDALFIHPCGFTELKFFGERSSYVDYKALTHSKAFLTEWARRLQYVYHLDYTSS
                     PAGYATSSYADEQLGKLSTEELKQMYAEKGIGYLITYRTSPAPDLPVMASNDTYVLYR
                     IAP"
     gene            complement(4655..5917)
                     /locus_tag="PK742_03175"
     CDS             complement(4655..5917)
                     /locus_tag="PK742_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75620.1"
                     /translation="MKQPALTRAFQFYQVCRYGALVLIGVILAKTGFSTSEIGVFETV
                     ILVSGVTSFFWLNGLLNTYVAQSSSQGSGTRALYSGTAVLTLFVIVLLILARPLLSDW
                     LALDDTGFFLLLLFILLNNTSFITEHILLVREQARSLTGLALAHAILLPLMVLLAALF
                     LNGMEAVILAMCLFLLGKNLFLLLLKPFTPNGGSTRELLLSASPLIGSFFFGGISVYV
                     DGIIVNSHFDKGAFAVYQYGAREFPVALLLANAFSMVMVRALGQHRSDGLRETKAGSL
                     RMMHRLIPLGILLMLSSKWIFPAVFNAQFSDSYVYFNIYLLLLIPRLIFPQSVLIAGG
                     QTRVQLYISIAEFVVNITSSLLLMQVMGLAGIAFGTVIAFVLEKLCMVFYLQHQGIQP
                     GSYIPLNWLMLYSFLLLSVFLMSTLAVL"
     gene            complement(5914..8028)
                     /locus_tag="PK742_03180"
     CDS             complement(5914..8028)
                     /locus_tag="PK742_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQU75621.1"
                     /translation="MTNESMAVDLEVQGMTCSNCALGVTRFLEKKGMKDVYVNFATNE
                     VRFHMPENEVSVRDIVNGIQELGYQVVQQDMAKPKKIGDIERKFLFSALFTIPLLLSM
                     IPGIPWLDNMYVQLVLATPVFLLGVWHFGRSALASLRTGVPNMDVLIFIGSTAAFTYS
                     LAGTILQLGHDYMFYETAATIITLVLLGNVIEKRSVKRTTTAIAELQKLQPDTARRLF
                     FDLASGEQTTMEVSVSDLRVNDLVQVNDGDRIPLDAVVKSGQMEVNESMITGESIPVV
                     KQAGDLVTGGTIIISGQAQLRIASVQQDGLLNNIIQLVKDAQTKKPSVQKLADRIAAW
                     FVPVVLSIAVLTFFISWFAVDVSLSGSLLRSIAVLVIACPCAMGLATPTAVMVGLGRS
                     VKQGILIKGGSTLEELARTEILVLDKTGTLTDGQFHIDRTDILSGDKKEIFSAVAALE
                     RHSSHPIAKALQAHFGPMSEPVESGDIQETKGVGLKGLINGNSWQVGNKLILHTPADE
                     TYDVYVTCNDQLVAGIRLADQVKQEAAAAVRYFKEQGIKTVLLSGDRSDTCATVAEQT
                     GMDSWLAEQTPASKLDWIREQSSGKKLTMVGDGINDSPALAAAHTGVSLSDASAAAIH
                     SAQVVLLDGDLSKLVMSHKLSRMTLQTIKQNLFWAFFYNVLAIPVAAAGFLSPMIAAL
                     SMAFSDVIVIGNSLRLRTRKIR"
     gene            8133..8906
                     /locus_tag="PK742_03185"
     CDS             8133..8906
                     /locus_tag="PK742_03185"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQU75622.1"
                     /translation="MENKKSSRRTFIQQSVLAAAGLGLAGKVQAAGWGDPGADDGING
                     FRIYPADGEFTLPALGYANNALEPHIDEMTMMIHHDKHHAGYVAKLNAAIDKAPELRG
                     RSLDQLLKNVGGLPEEVRTAIRNNGGGHWNHSFFWQVMSPNAAASMPSDALRNAMIAT
                     WNDMDTFKAEFKKTASGVFGSGWAWVIKDQQGNLKLTSTPNQDNPLMDTSAERGMPIL
                     GIDVWEHAYYLKNQNRRGDYIDSFLQVVDWDFVSKQFGK"
     gene            8963..9036
                     /locus_tag="PK742_03190"
     tRNA            8963..9036
                     /locus_tag="PK742_03190"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8997..8999,aa:His,seq:gtg)
     gene            9069..9911
                     /gene="prmC"
                     /locus_tag="PK742_03195"
     CDS             9069..9911
                     /gene="prmC"
                     /locus_tag="PK742_03195"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQU75623.1"
                     /translation="MSRLSAYRQQAVQQLTGLYEAQEAASIVRIWLEERLGERPLSPV
                     RELMAEEKRMLESDLQKMMTGEPLQYVLGKTWFDRLTLAVDNRVLIPRPETEELVHWI
                     VEEHPGFSGNILDIGTGSGAIALALKDRLPDALVFACDTSDEILDLAAENSNNSKLQL
                     VLLQADVLQSEAWNAFPSVQVIVSNPPYIPLYELAEMHVNVGEHEPHQALFVPDDDPL
                     LFYRTILTEGCKKLLPGGYFYFEIHEQMEQQLISLLTELGLLHFQFRKDMQEKVRMLR
                     VHNV"
     gene            9960..10241
                     /locus_tag="PK742_03200"
     CDS             9960..10241
                     /locus_tag="PK742_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75624.1"
                     /translation="MFNRLKEKWGVSWGRFALIFLTFALGGSACGYLAEIALEWLHIP
                     SRAVRIPVYIVLVTLLWPLCVLAISIVTGQFTFFRAYIRKIIARMGGRK"
     gene            10304..11059
                     /locus_tag="PK742_03205"
     CDS             10304..11059
                     /locus_tag="PK742_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQU75625.1"
                     /translation="MSWNIRYDHAGDSLHAWADRKQPMADWLQNSGAEVIGIQEGLFH
                     QVQFLDSVLTNFTYVGAGREDGAQAGEFSAIFIDTTRFFVLESSTFWLSETPGEVSVG
                     WDAALPRVCTYALIQDKRTNKMCWVFNTHFDHMGGKAREEAARLIVSRIAVKNNTTRC
                     PVVLTGDLNAEPKDPPLKVLGAIMSDGYISFAPHAVRGTWNAFDCEQIHDKRIDYIMV
                     NGTTFSAYDHILLCDPEIGALSDHWPVLAELRF"
     gene            complement(11075..11998)
                     /locus_tag="PK742_03210"
     CDS             complement(11075..11998)
                     /locus_tag="PK742_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQU75626.1"
                     /translation="MNYTLNQLQIFLKIVETESVTKAAGQLYLSQPAVSMQLKKFQDQ
                     FDIPLTEVVGRKIYITDFGREIAAAAEQIILQVDAINHRTLAYKGQLAGRLKLSVVST
                     GKYVMPYFLSDFMQQHSGVELIMDVTNKSKVIESLENNEVDFSLVSVLPTGLQVEKLD
                     LLQNKLFLVGNAKAKFGKEIRGKDIFRDLPLIFREPGSGTRQTMEKFIERHHIAAVKK
                     MELTSNEAVKQAVLAGLGFSIMPLIGIRNELRNKELQVIPVRGLPIKTNWSLIWLKGK
                     KHSPASEAYLAHLRKERTGIVQETFSWYEEF"
     gene            12085..13521
                     /locus_tag="PK742_03215"
     CDS             12085..13521
                     /locus_tag="PK742_03215"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006673.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQU75627.1"
                     /translation="MHKITIAKGDGIGPEIMEATLDILRSAGAPLAFEEIEIGEKVYL
                     SGNTSGISTEAWDTIRHNKVFLKAPITTPQGGGYKSLNVTTRKMLGLYSNVRPCRSLH
                     PFIATRHPGMDVVIIRENEEDLYAGIEHQQTDEVVQCLKLISRPGCEKIVRYAFEYAR
                     QQGRKKVTCFTKDNIMKQTDGLFRKVFNEIATEYPEIASEHWIIDIGAAKLADTPETF
                     DVIVTPNLYGDVVSDIAAQITGSVGLAGSANIGEECAMFEAIHGSAPDIAGKDLANPS
                     GLLQAAVMMLNHLGETAVAEQIQNAWLRTLEEGIHTADIYREGVSTLKVGTRAFAEAV
                     IHNLGKKPEQLKPAASAGSLMLNLPRYQRKQPAQKKLEGVDVFIHWAGYDANELAEKL
                     HRMEAGKLRLTMITNRGIKVWPNGFKETFCTDHWRCRFQPTDGEQLSNKHILELLAHA
                     VQDDIDTIKTENLYSFDGRPAYSLGQGQ"
     gene            13543..13719
                     /locus_tag="PK742_03220"
     CDS             13543..13719
                     /locus_tag="PK742_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75628.1"
                     /translation="MNHVSIGLLLSFTAMVICLGLAIVSHDVVNSRLFAGLSLVAAVL
                     TMRLLTQTNSSRYE"
     gene            13712..13993
                     /locus_tag="PK742_03225"
     CDS             13712..13993
                     /locus_tag="PK742_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75629.1"
                     /translation="MNRTEQYTLIDGTFDAAEAGDILYDLFSFKINYHERKNFSSQER
                     FGVDDANAVRRLPELRQTLKQIREQVASAVAADKKLIVHSEVLITITEI"
     gene            14073..15038
                     /locus_tag="PK742_03230"
     CDS             14073..15038
                     /locus_tag="PK742_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HQU75630.1"
                     /translation="MDLNLLIDNLTNPALLFFVLGIIAVWVKSDLEIPPTSSKFISLY
                     LLFAIGFRGGQELSHEPFSTEIGLSMLLGIGLSVLIPLYTFFILKRKLNTWDAGAIAA
                     AYGSVSAVTFVTAVSYLESQGLHLHGYMVAVMALMESPAIIMGLVLISMYNKDDADTQ
                     ISKRSAIKHSFTNGSVLLILGSLVIGMLADAQQAEGIKPFTNDLFKGFLAIFLLDMGI
                     ISARKMKVFLSVGLFPVLFAILIPLFNGSLVALLSGLVTDDVSNRFIFAILAASASYI
                     AVPAAMKVSVPKANPGHFLPMALAITFPVNITIGMPVYFSIIQCA"
     gene            complement(15045..15410)
                     /locus_tag="PK742_03235"
     CDS             complement(15045..15410)
                     /locus_tag="PK742_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HQU75631.1"
                     /translation="MHPLSQILVAIVAAEHLFILYMEMFAWETLGRKVFRGALPKELF
                     TPTKVMAANQGLYNGFLAGGLIWSLFIQDAVWQQHVATFFLCCVVVAGLYGGFSASKR
                     IIITQALPAAVALCVLLLA"
BASE COUNT         4038 a         3933 c         3904 g         3850 t
ORIGIN      
        1 gatacgaaag cagcttacct gatagaactg acggttacgg atgacctggg gtgtatcacc
       61 tcttacaaag acaccgtgat cgttacacag cctgtggccg attttgtatt gggagataca
      121 gttccctgcc tggatgagat cgtcgtattt gaagaactgg ctaccggaag cggtatcacc
      181 tacctctggg attttggcga tggtactacg tcaactgagg ccaacccgga acacagttat
      241 ggatcttatg gcgcatttga tgtgaccctg atcgtaaccg atgataacgg ttgttctgat
      301 actaccacca agatcgctta tgtgaatgtg gactccattc tgggtgatat cacctatgat
      361 gagctggtgt ccagctgcaa ctactctctg gtgcagttct atgtgaccac agatgatacc
      421 atttgctctt atgactggga ttttggcgat ggctttggcg gctccggaac ctccagccct
      481 atctatcctt atctggaagc cggtatctat gatgtgagtg ttaccctgac cacctgctcg
      541 ggatgcgtgg gcacgctgac ggaagaagac ttcattcttg tgcccggacc ctacgggtcg
      601 ctggaaccac tggaagatac cgtgtgcatt ggcaatgatg ccattttcca gctgaatttt
      661 tcatctactg ataccgccac cattttctat ggcaacggcg attttggtga actggacctg
      721 ccttatcagg atgagttgga ttccatgttt ctggactatg cctatgacac atcaggcatc
      781 tatgtggtgt ctatcctgct ggttgatacc aacggttgtt tcaatgtact ggatcttcct
      841 gacagcctct gggtaggatt cccccccaac agcatttacg cagttgacag cggcggttgc
      901 ctgggaactg agttcttctt tgatgaaggc tctaccggcc ttgacccgat cgtttactgg
      961 tcctgggata ttggcgattc cgtatttgtt gatacttccg gggcagattt ctattatacc
     1021 tacacggaag caggtattta taacacctct cttattgtat ctactgccct gggctgcgca
     1081 gatacactgg agtttacagt tggtgttctg gatacaccta cggtgaacat ctcaccggat
     1141 accgtgatat gtccgggtaa ctttgttact ctcagtgccg aagatggcat cgtgtactcc
     1201 tggtcgccgc ccgacggact ggatgacccc accagtgata cacccagtgc ttcgcctgat
     1261 gcaaccacta cctattctgt tgtggtcagc aacggctact gtgaagcatc tgataccgtt
     1321 caggtgcggg tgctggaaga actgatcacc aatgccggac cggataccgc attgtgcctg
     1381 ctgggagggg tacagctata cggtgaactc gtgtctgatg taccacctga tcagatcgaa
     1441 ttcacctggt tcccgcccac agaactttca gcgacagata ttctggatcc atattccagt
     1501 tcggccgatg atatcaccta ctatctcatt gccagttgtg gcaccctgag tgatacagca
     1561 gatgcacaga tccaggtggt ggcacctcct gatgtagaga tcgcggttga cagcatcctg
     1621 ctgatccagg acgaagtagc acagatcaac gccagtctgt taagctccag ttcggcggtt
     1681 acctataact ggttcccgcc tgatcagatc aactgcaata attgttcttc tgtttttgtt
     1741 tcacctgatg tgaccactgt ttacagtgtg gaagtgatag atgagaacgg ttgtaccgat
     1801 attgattttg tctatgtacg tgtcatgccc tgcgatgaga ccctgatgtt catccccaat
     1861 atcatcagcc ccaacaacga cggcatgaat gacattttcc gcatcaccta cgaaggcatc
     1921 acgttgatcg aacacatata tgtatatgac agatggggag agctggtggc agaatcaaag
     1981 gatcccaaca agatctggga cggcacctat aagggagagc tctgtaaccc cggtgtctat
     2041 gtgtatgtca tcgaagcgat ctgtgtgaat gatgcgccca atatcattac aggtaacata
     2101 accctggtgc gataagaagt aagcatgaac aaggtgaggt ccgtaatact gttgtgcatg
     2161 ctgtgggtgg tctcctgtaa agtgcaggcc caggactggc attactccac tttccattcg
     2221 gctccaacca tgatcaatcc tgccttcacc gggctgttca acggtgacct gcgcatcatt
     2281 ggaaactacc ggagtcaatg gcagtttgcc aatgcctatg agaccttctc tatggcggct
     2341 gacggagctt ttgctgtcat caacaacggc gattttgcca gcgctggact taccatcctg
     2401 gcagatcagg ccggcgatct gaattttacc accatgcata ctgctgccag tatggcctac
     2461 tccaaagcac tcaatggcta tggcaaccag tatctgaccc ttggtctgca ggcagctgtc
     2521 aacagacgct ccatagatta taccaacatg gtagccttcg atcctgaacc atttgctact
     2581 gatgtccagg atcattattc atatctggac atgaccgtgg gtgccagctg gtattttctt
     2641 cccagcgatg agatctacat ttttcttggc ggtgcctggt ttcatttcaa tgaacccaac
     2701 cagagctttt tgaacaactc tgaagatatt ctggaaagca gatactccct gcatggcgga
     2761 atgcagttcc ctgtaatgga cagaatgaac atcctgccca gtgtcatgta taatatgcag
     2821 ggacaataca cggaattttt gctgggaagc tatctgaaat tcaagctcaa ccatgtgacc
     2881 gtcaatgacg aaacagcatt gtatgcaggg gtatggtacc gcacgtctga tgctgcggtg
     2941 gtgagtgtca ggtatgatta caacactact gtatttgcac tcagctatga tgtcaatacc
     3001 tccgatcttt cttctgccag ctatggacgc ggagggctgg agctgaatgt catttatgtt
     3061 ctggacaaaa agttcagcat cagcaagaga cttaagaacc ggagttattc gtgcccgaaa
     3121 ttctaaggag ctatccggta taagacatag gtatcattgg aagccatgac cggaagatca
     3181 ggagcaggag atgttctgta cgtgatcaga taaccgatgc ctttttctgc atacatctgc
     3241 ttcagttctt ctgtgctcag cttacccagc tgttcatctg cataggaact ggtggcgtaa
     3301 cctgcaggag agctggtgta atccagatga tagacatatt gcaaacgacg ggcccattcg
     3361 gtcagaaagg cctttgaatg ggtcagggct ttatagtcca cataggaaga acgttcgccg
     3421 aaaaatttca gctctgtgaa gccacaggga tgtatgaaca aggcatcggt cggggtcagg
     3481 gcttttgcct gcctgcttat gtctatcgca tcatccgtgt aatcacgaaa gggcaggtca
     3541 taatagggtg tagcttgtct gtatatattt ccgggactga tcagcgcacc ccagagaaca
     3601 ggcaataaca ggatgacggg cagtgctgct ttgtgcaggg ttgcgggcat gcggtccaga
     3661 atgttttctg ccatgtggag caacgctatg acagacaaca tctccagcca gatggttgac
     3721 ttgaaccatt gcaaagtggt gatggtctcc gatctcattt gtacaccggc acaatatatt
     3781 cccagtgaga taaaagcagc aacaaagaac cagcccagtc tggactcgcg gaagaaccag
     3841 atgatactac ccagaatgat aagaggagtg agtatcaccc agttccgttg gccgaatgca
     3901 gccggaaggt agtggtgggg atttctgaat acatagaaga tctgatacag atccatgccc
     3961 tccggcacca caccggtttc aaagcgacgg tgcaccagaa tgagaaacaa tcccgcagtg
     4021 caaaaccaga tcagtatgcc tatgaacagc cgcttgggca gccagctctt cttgtcggtg
     4081 atggaccaca acgcttcgcc ggcaacaaca agactgaata cctgcagtcc cgccacagga
     4141 tgaaaaagcg tggatagtgc agcaactgac aaagccagga tcacccgtct gcgcagccag
     4201 aaaacgatac tccagatgcc aatggatttg gcaaccagac tgggatgaaa aatgccgtaa
     4261 tacagttcat tctgacccag gttgatgcca tacaagggta tgaatacaat aagcggaaca
     4321 gccagccgca gccaggtgct gtgcaggtac cgcgaagaca gacgatacag gcccatcagc
     4381 agcagcaaac tgaaaaagac gtggtacaga aaactcaccc actcaagatg accggtaaat
     4441 ggcctgagca acaacagaaa aaagaatcgt tcattgggga aggtggactc tgcctcctgc
     4501 acatacaggt cgttcggata gagctccgga tgcgctgcat gtaccatcag cggatccagc
     4561 tgcaccagat caccacgacc gaactgatac ccccagctta ctaccagtaa cacataggtg
     4621 agcagggtac aacagatgat gatgagtttc cggttcataa tacagccagg gtgctcatca
     4681 aaaatacact taacaacagg aaggaataga gcatcaacca gttgagcggt atgtagctgc
     4741 ctggttggat gccctggtgt tgcaggtaga ataccataca aagtttctcc agtacgaagg
     4801 cgatcaccgt tccgaatgca ataccggcaa gtcccatgac ctgcatcagc aacaagctgc
     4861 tggtgatatt caccacaaat tctgcaatgg aaatatatag ttgaacacgt gtctgcccgc
     4921 ctgctatcag aactgactga ggaaatatca gtctgggtat gagcagcagc agataaatat
     4981 tgaaatacac ataagaatcg ctgaactgcg cattgaatac tgccggaaag atccatttgc
     5041 tggatagcat cagcaggatg ccaagaggta tcagccggtg catcatgcgc agactaccag
     5101 cttttgtttc gcgaagtccg tcagaccggt gctgaccaag tgccctgacc atgaccatgc
     5161 tgaaagcatt ggccagcaac aacgctaccg gaaattctct ggcgccatac tgatacacgg
     5221 caaatgcccc tttgtcaaaa tggctgttca ctatgatacc atccacgtaa acagagatac
     5281 caccaaagaa aaagctgcct atcagcggag aggcagaaag cagcaactct ctggttgagc
     5341 caccatttgg tgtaaagggt ttcaacagca gcagaaacag attctttccc agcagaaaca
     5401 gacacatggc cagaataacg gcctccattc cgttcaggaa cagcgcagca agcaggacca
     5461 tgagcggcag caaaatggca tgcgccagcg ccagtccggt caggctgcgg gcctgttctc
     5521 ttacaagcag aatatgctct gttataaaag aggtgttgtt cagcaggatg aacagcaaca
     5581 acaaaaagaa tccggtatca tccagtgcca gccagtcgct gagcaaaggt ctggcaagaa
     5641 taagcaatac gatcacgaag agggtaagaa cagcagttcc cgaatacaaa gcccgtgtac
     5701 cactgccttg tgaactgctc tgagctacat atgtattcag cagtccgttc agccagaaaa
     5761 aactggtcac accggaaacg aggatgaccg tttcaaatac accgatctct gaagtgctga
     5821 atcctgtctt tgcaaggatc acaccgataa gtaccagagc accgtatctg catacctggt
     5881 agaattgaaa tgctctggtc agtgctggct gcttcatctg atctttctgg ttctcagccg
     5941 caggctgttg cctatcacaa tgacatctga aaatgccata ctgagtgccg cgatcatcgg
     6001 actcaggaaa cccgctgctg ccaccggaat ggccagtaca ttatagaaga atgcccagaa
     6061 cagattttgt ttgatggttt gcagggtcat tctactgagc ttgtgcgaca tgaccagttt
     6121 gctcagatcg ccatccagca ataccacctg tgcactgtga atagctgcgg cgctggcatc
     6181 actcaggctt acaccggtat gcgctgcagc cagggcagga gagtcgttga taccatcgcc
     6241 caccatagtg agtttcttac cggaactctg ctccctgatc cagtctagtt tggatgccgg
     6301 tgtttgttct gccagccatg aatccattcc cgtctgttct gctactgttg cacaggtatc
     6361 agaacggtcg ccgctgagca acaccgtttt gatgccttgt tccttaaaat acctgactgc
     6421 tgctgctgct tcctgtttta cctgatcagc taaacggatg cctgccacca gctggtcatt
     6481 acaggtcaca tatacatcat aggtttcatc ggccggagtg tgcagtataa gcttgtttcc
     6541 cacctgccag gaattgccgt tgatcagacc ttttaatccc acacctttcg tctcctgtat
     6601 atcgccggat tctacgggtt cagacatcgg accgaaatgt gcctgtaacg ctttggctat
     6661 aggatgagaa ctgtgtcttt ccagcgcagc tacagctgag aatatctctt ttttatcgcc
     6721 tgaaagtata tctgttctgt ctatatgaaa ctgtccgtcg gtcagcgtac ccgttttgtc
     6781 cagcaccagg atctctgttc ttgcaagttc ttccagtgtg ctgccgcctt taatgagaat
     6841 gccctgtttc acacttctgc caagccctac catcacggct gtaggagtag caagtcccat
     6901 ggcacacggg caggcgatca ccagtacggc aatgctgcga agcaaagaac cggacaggga
     6961 aacatccaca gcaaaccagg aaatgaaaaa ggtgaggact gcaatactca gcaccaccgg
     7021 tacaaaccag gctgcaatgc ggtcggccag tttctgtacc gatggctttt tggtctgcgc
     7081 atccttgacc agctgaatga tattattcag caggccgtct tgctgcaccg aggcaatgcg
     7141 caactgagcc tgaccactaa taatgatggt accaccggta accagatcgc ccgcctgttt
     7201 gaccaccgga atgctttcgc cggtgatcat gctttcattc acctccattt gtccggactt
     7261 aacgactgca tccagcggaa ttcggtcgcc gtcatttacc tgcaccagat cattcactcg
     7321 aagatcgctc acagagacct ccatggtggt ttgttcgcca ctggccagat caaagaacaa
     7381 tcgcctggca gtatccggtt gtaatttttg caactcagca atggcggtag tggtcctttt
     7441 gacactgcgc ttttctatta cgttgcccag caacaccaaa gtaatgatgg tggcagccgt
     7501 ttcatagaac atataatcat gcccgagttg caggatggta cctgcaaggc tgtaagtaaa
     7561 tgcagccgtg ctgcctatga aaataagcac atccatgttg ggtacacccg tacgtaaact
     7621 ggccagtgca ctcctgccga aatgccacac acccagcaga aaaaccggcg tggccaatac
     7681 cagttgcaca tacatattgt ccagccaggg aatacccggt atcatggaaa gcagcaaagg
     7741 gatggtgaac aaggcgctga acaggaactt gcgttctatg tcgcctatct ttttcggctt
     7801 agccatatcc tgctgaacga cctggtaccc cagttcctga ataccgttca cgatgtctct
     7861 tacagacacc tcattttccg gcatgtgaaa gcgcacctca ttggtagcga aattcacata
     7921 cacatccttc atgccttttt tctcgagaaa gcgtgtcaca ccgagtgcac agttggaaca
     7981 ggtcattccc tgcacctcca gatcgaccgc catagattcg ttggtcatac agtatgaaca
     8041 caaatttagc caaaatggtt accttcatgt atcccgtctg gcatacacag acatacccga
     8101 cctcttattc tttcatgaca aaccgtaaaa aaatggaaaa caagaaatct tccaggagaa
     8161 cctttattca gcaatccgta ctggcagccg caggcctggg actggcaggc aaggtgcagg
     8221 ccgcaggatg gggagatccg ggtgccgatg acggcattaa cggcttccgg atctatccgg
     8281 ccgatggtga attcaccctc ccggcgcttg gttatgccaa caatgccctg gaaccgcaca
     8341 tagatgaaat gaccatgatg atccatcatg ataaacatca tgcgggttat gtggccaagc
     8401 tgaacgcagc catagacaag gctcctgaac ttcgcggccg cagcctggac cagttgttaa
     8461 agaatgtggg cgggttgccg gaagaagtgc gaacagccat tcgaaacaat ggaggtggcc
     8521 actggaacca cagctttttc tggcaggtaa tgtcgcccaa tgctgctgcc tccatgccat
     8581 cagatgcact gcgcaatgcc atgatcgcca cctggaacga tatggacacc tttaaagcag
     8641 aattcaaaaa gaccgcttcg ggtgtattcg gttccggatg ggcctgggtc attaaagatc
     8701 agcagggaaa tctgaaattg accagcacac ctaatcagga taatccgctg atggatacca
     8761 gtgcagaaag gggtatgccc attctgggaa tcgatgtctg ggaacatgcc tattacctga
     8821 agaaccagaa ccgccgcgga gattacatcg acagttttct ccaggtagtt gactgggact
     8881 ttgtttcaaa gcagttcgga aaataggccg gtcatttgca gaaaaatgct gcagatgatg
     8941 taattttgcc cacctcttca cggtgactgt agctcagtcg gttagagcac tagattgtgg
     9001 ttctaggggt cgtgggttcg aatcccatca gtcacccaaa gccctgcaca tgcagggctt
     9061 ctttatttat gtctcgtctc tctgcatatc gacagcaggc tgtgcagcag ctgaccggtc
     9121 tctatgaggc acaggaagct gcttccatcg tgcgcatctg gctggaagaa aggctgggag
     9181 aaaggccgct ttcgcccgtc agagagttga tggcggaaga aaaaagaatg ctggaatccg
     9241 acctgcaaaa aatgatgacg ggtgaaccct tgcaatatgt gttggggaag acctggtttg
     9301 acaggcttac gcttgcggtc gataacaggg tcctgatacc aagacctgaa acggaagaat
     9361 tggtgcactg gatcgtggaa gaacaccctg gcttttcagg aaatatactg gacatcggaa
     9421 ccggcagcgg tgccattgcc ctggcactga aagacagact tccggatgct cttgtttttg
     9481 cctgcgacac ttctgatgag atacttgacc tggctgcaga aaacagcaac aacagcaagt
     9541 tacaactggt gttgttgcag gccgatgtgt tgcaatctga agcatggaat gcgtttccct
     9601 ccgtgcaggt catcgtcagt aatccaccct atataccgtt gtatgaactg gcagagatgc
     9661 atgtgaatgt aggcgaacat gaaccgcatc aggccttgtt cgtgcctgac gatgatccgc
     9721 tcttgttcta ccggaccatt ctgactgaag gctgtaagaa actgttaccc ggtggctatt
     9781 tctattttga gatccatgag cagatggaac aacagctcat ctctctgcta acggaacttg
     9841 gcttgcttca ttttcagttc agaaaagata tgcaggaaaa agtgcgcatg ctgcgtgtgc
     9901 acaacgtata acttggaaac ggatggagcg gggcgtagtt ttgcccgcag gtaaaataca
     9961 tgttcaaccg attaaaagaa aagtggggtg taagctgggg caggttcgcc ctgatcttcc
    10021 tgaccttcgc cctgggcggc agcgcctgtg gttatctggc agagatagca ctggagtggc
    10081 ttcacattcc ttccagggct gttcgcattc cggtatatat tgtacttgta accctgctct
    10141 ggcctttgtg tgtgctggcc atttctattg taacaggcca gtttactttt ttcagggcat
    10201 acatacgaaa gatcatcgcc cgcatgggag gtagaaaatg aggtcattcc tgttcattgt
    10261 atcactttct ttttcactgt gtacgcgatc acaggtcagt gtggtgtcct ggaacatccg
    10321 ttatgatcat gcgggtgatt cgctgcatgc ctgggccgac agaaaacaac ccatggccga
    10381 ctggctccaa aacagcggtg cagaagtgat cggtattcag gaaggactgt ttcaccaagt
    10441 gcagttcctg gattctgtgc tgaccaattt tacttacgtg ggagcgggca gggaagatgg
    10501 cgctcaggcc ggagaatttt ctgccatatt catagacaca acccggtttt tcgtcctgga
    10561 atcttccact ttctggctgt ctgaaacacc cggcgaagta tccgtaggat gggatgcagc
    10621 cctgccccgt gtgtgcacct atgcattgat acaggataag cgaacgaata agatgtgctg
    10681 ggttttcaat acgcattttg atcacatggg cgggaaggca agggaagaag cagccaggct
    10741 gatcgtaagc aggatagcgg tgaagaacaa cacgaccaga tgtccagtcg tcctgaccgg
    10801 cgacctcaat gccgagccga aggatcctcc gctaaaggtg ttgggtgcga tcatgtctga
    10861 cggttatatc tccttcgctc cacatgctgt tcgcggcacc tggaatgcct tcgactgtga
    10921 acagatccac gacaagcgga ttgattatat catggtgaat ggcaccacct tctctgcata
    10981 cgatcacata ctgctatgcg atccggagat cggtgcattg tctgaccact ggcctgtact
    11041 ggctgagctc aggttctgaa ggcatgcggg gaggtcagaa ctcctcatac cagctgaatg
    11101 tttcctgcac gatgccggtt ctttccttcc tcaaatgtgc cagataggct tcagatgccg
    11161 gcgaatgttt cttccctttc agccagatca gactccagtt ggtcttgatg ggaagacctc
    11221 ttaccggtat gacctgaagt tccttgttcc gcagttcgtt gcgaatgccg atcagaggca
    11281 tgatagagaa ccccagcccg gccagtacag cctgttttac tgcttcgtta gaggtgagct
    11341 ccattttctt cacagctgca atatgatggc gctcaatgaa tttttccatg gtctgtcgtg
    11401 tgcctgatcc gggctctctg aatatgagcg gcaggtctct gaagatgtcc ttccccctga
    11461 tctcttttcc gaattttgcc ttcgcattac ccaccagaaa cagtttgttc tgcaacagat
    11521 ccagcttctc cacctgtaaa ccggtgggca gtacagacac cagcgaaaag tccacctcat
    11581 tgttttccag actttctatc accttgctct tgttggtcac atccatgatc agttccacac
    11641 cgctgtgctg ctgcatgaag tcactcagaa agtaaggcat cacatatttt ccggtagata
    11701 ccacagatag cttgagtcgg cctgccagct gtcctttgta agccagggtg cggtgattga
    11761 tggcatctac ctgaagaatg atctgctctg ctgcagcagc gatctccctg ccaaagtctg
    11821 tgatatagat ctttctgcct accacttccg tgaggggtat atcaaactga tcctggaatt
    11881 ttttcagctg catggacacg gcaggttgag acaagtacaa ctgcccggca gcctttgtca
    11941 cgctttccgt ttctacgatc ttcagaaaga tctgtaactg attcagggta taattcataa
    12001 gtaatactta tgtaaactat cacaaagata tattttcact tatgaaaatc ctgattcaat
    12061 tttgcggcag ttaaaaacaa tcatatgcac aagatcacaa tcgcaaaagg agatggcatc
    12121 gggccggaga tcatggaagc cacactggac attctgagat cggccggagc accgcttgca
    12181 tttgaagaga tagagatcgg tgaaaaggtt tatctgtcgg gaaacacctc gggtatttca
    12241 acagaagcct gggataccat ccgccataac aaggtattcc tgaaggcacc catcaccaca
    12301 ccgcagggtg gcggctacaa aagcctgaat gtgaccacca gaaagatgct ggggttatat
    12361 tccaatgtaa gaccctgcag aagtctgcat cccttcatcg ctaccagaca cccgggcatg
    12421 gatgtggtca tcatccgcga gaacgaagag gatctgtatg ccggtatcga acaccagcag
    12481 acagatgagg tggtgcagtg tctgaagctg atcagcaggc cgggttgtga gaagatcgtt
    12541 cgctacgcat ttgaatatgc aaggcagcag ggaagaaaaa aggtgacctg tttcaccaag
    12601 gataatatca tgaaacagac agatggtctg ttccggaaag tgttcaacga gatcgccacg
    12661 gagtatcctg aaattgcaag tgagcactgg atcatagaca tcggggcagc caaactggct
    12721 gatacgcctg aaacatttga tgtgatcgta acaccaaacc tttatggcga tgtggtgagt
    12781 gatatagcgg cacagatcac cggctctgtg gggctggccg gttctgccaa tataggagaa
    12841 gagtgtgcca tgttcgaagc catacatggc tctgctcctg atattgcagg gaaagacctg
    12901 gccaatcctt caggactact gcaagcggcg gttatgatgc tgaaccacct cggcgagacc
    12961 gctgtggcag aacagataca gaatgcctgg ctgcgcaccc tggaagaagg catccataca
    13021 gctgatattt accgggaagg agtgagcact ttgaaagtcg gaaccagggc ttttgctgag
    13081 gcggtcatcc acaacctcgg gaaaaagccg gagcaactga aacccgcagc atctgccggc
    13141 agcctgatgc tgaacctgcc caggtaccag cgtaagcagc cggcgcagaa aaagctggaa
    13201 ggggtagatg tgttcataca ctgggcaggc tatgatgcca atgaactggc agaaaaactg
    13261 caccggatgg aagcaggtaa gctgcggctc accatgatca ccaacagggg tatcaaggtc
    13321 tggccgaacg gtttcaaaga gaccttctgt accgaccact ggcgatgccg attccagcca
    13381 acagatggag aacagctgtc taacaagcat atactggagt tgcttgcgca cgccgtacag
    13441 gatgatatag acaccatcaa gacggagaac ctgtattctt tcgacggaag acctgcctat
    13501 tcactgggac agggacaatg atcaaaataa aaaagtacgc acatgaatca tgtatccata
    13561 ggactgttgc tcagctttac agcaatggtc atttgtctcg gtttagccat cgtttcacat
    13621 gatgtggtaa acagcagatt gttcgcagga ttatcgctcg tggcagcagt cctcaccatg
    13681 cgattgctta cccaaaccaa cagcagcaga tatgaatagg acagaacagt acaccctgat
    13741 agacggcact tttgatgcag cagaagccgg agatattcta tacgatctgt tctctttcaa
    13801 gatcaattat catgaaagaa agaacttcag ttcgcaggag cggttcgggg tggatgatgc
    13861 caatgccgtg cggcgtttgc ccgagctgcg ccaaaccctg aaacagatca gggagcaggt
    13921 agcatctgcc gttgctgccg ataagaaact catcgttcac tctgaggtgc tgatcaccat
    13981 caccgagatc tgataattcc tgtatcactt atttcatttt gctgagcggg tcatagcgcc
    14041 cgctcagcct gaatatgcct gaaagtttac caatggacct gaacctgctg atagacaacc
    14101 tgaccaatcc cgccctgcta ttctttgtgc tgggcatcat cgccgtatgg gtaaagagtg
    14161 atctggagat cccacccaca tcatccaaat tcatttccct gtacctgttg tttgccatcg
    14221 gcttccgggg cggacaggaa ttatcgcacg agccattcag cacagagatc ggactgtcca
    14281 tgctgctggg aattggcttg tctgtcctca ttcccctgta cacctttttt atactgaagc
    14341 gaaaactcaa tacctgggat gcaggtgcca ttgctgcagc atacggatcg gtcagtgcgg
    14401 tcacttttgt aactgcagtt tcctatctcg aatcacaggg gctccatctg catggctaca
    14461 tggtagcggt catggcgctg atggaatcgc cggccattat catggggctg gtgctgatct
    14521 ccatgtacaa caaggatgat gcagacacgc agatcagcaa acgcagcgcc atcaaacatt
    14581 ccttcaccaa cggaagtgtg ctgctgatcc tgggcagcct ggtcatcggc atgctggcag
    14641 atgcacaaca ggcagagggt atcaagccct ttaccaatga tctgttcaaa ggttttctgg
    14701 ccattttcct cctggatatg ggcatcatca gtgccagaaa aatgaaagtg tttttgtcag
    14761 ttggcctttt tccagtcttg tttgccatcc tgatcccact gttcaacgga agtctggtgg
    14821 ccttgctcag cggtctggtg acagatgatg tgagcaaccg tttcatcttt gcgatcctgg
    14881 cagccagcgc atcatatatt gcagtccctg ctgccatgaa agtatcggtg ccaaaggcca
    14941 atccgggtca ctttctgccc atggcacttg ccattacctt cccggtcaat atcaccatcg
    15001 gcatgccggt ctatttcagc attatccagt gtgcttaagt tctttcaggc aagcagcagc
    15061 acacatagtg ccacagcagc aggcagtgct tgtgtgatga tgatcctctt gcttgcagag
    15121 aagccgccat acagtccggc taccaccaca caacataaaa agaaggtagc aacatgttgt
    15181 tgccacacag catcctgaat gaacagagac cagatcagtc cgccggccag gaaaccattg
    15241 tacaatccct gattggccgc catcactttg gtgggcgtga acagttcttt gggtaatgcc
    15301 ccgcgaaaga cctttctgcc cagtgtctcc caggcaaaca tttccatgta aaggatgaag
    15361 aggtgttctg ctgcaacgat ggccacaagg atctgtgaaa gtgggtgcat aatggatcag
    15421 gttgatgata aatgtaaggg ttttgtcggg tatgccacgt cccgccggtg ttaccgaagc
    15481 tgcttcacac ataaacctcc atgatcattt ggcggaattc cattcctgat aagccaacgc
    15541 cctgtctgag ataacataat agattgctat aaataccaaa gatcagatgt actttgttcg
    15601 aagggaattg tctccccgga cggggagtac ccgaaggggg aggggtgatg tacacgcact
    15661 caacatttgt ttattgaaac acaaaccttc tggtttgaca aacaactaaa atacccctta
    15721 cttcg
//
LOCUS       DAOMAX010000033        15709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_419_length_15709_cov_4.961479, whole genome shotgun sequence.
ACCESSION   DAOMAX010000033 DAOMAX010000000
VERSION     DAOMAX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_419_length_15709_cov_4.961479"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PK742_03240"
     CDS             complement(<1..1524)
                     /locus_tag="PK742_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HQU75632.1"
                     /translation="MRTQLLAGALFLSAVSFAQTNVGTLQGKVTDATTGLPLPNVNVI
                     IMQDGQLLSAQGTSANGNYMYEKLYPNTFDVAFAVKGRQLENRTVTGVEIQAGMITTL
                     NVTIGDKDIIAQDLSIRPDPVTNPIALAIVLPVEKKSDEVKEEFKGQYYPFIPEQDFV
                     LTKEKANSTFSADVDNAFYTIVRRMIMHRVIPPQGAVRIEEMLNYFDYEYPKPDGSDI
                     SVYTESAACPWNQDHLLLHIGLQTREVFQDDLPPSHLVFLMDVSGSMATSDKLPLAKS
                     SMRTLVRQLRPQDRVSIVTYRETVEVVAEDLPGSDKEAILRALAGMYADGSTAGAPGL
                     EKAYDIAGKHFIRNGNNRIILCSDGDFNVGPTSDDALLQLVRSQRKNGVYLTVLGFGM
                     DNLQDGRLELLADNGNGNYGYIDQIEEARKLLVEEMGGTLVTVARDVKLEATFDERYV
                     KAWRLIGYENRALTDKEFDKRKTDAGEMGMGQTVTALYELIPADELLNNDGNMGNFII
                     "
     gene            1875..2372
                     /locus_tag="PK742_03245"
     CDS             1875..2372
                     /locus_tag="PK742_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75633.1"
                     /translation="MKRKHLLWAVLPLMALITGCPVGVDHPLGEPGKEKLDEQLIGTW
                     VQPDTAMEATRIAIEKIDKHSFKLTVLEEGGMFMEETDFFRGWCTKVDGYTFVYLQDL
                     NNPAGQYLTYCYRMDGDDLYTYDMTLAVGGIDAVTSTEAFRKEVSASLKLEGSLTSEV
                     KWSRQ"
     gene            2397..3131
                     /locus_tag="PK742_03250"
     CDS             2397..3131
                     /locus_tag="PK742_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQU75634.1"
                     /translation="MQNEATVFDDIVQRRRSMRIFSADAPMDEGAVQRSLQRALLAPN
                     SSNMQVWEFYRVSDPEKLQVLAKYCMGQRAATTARELVLVVVRRDLWKKRADYMVEFN
                     EQYYADKPELQHRLKRIKKYYRRLMPMYYFQDWFGIWGRMRQLIVFFASFRRPVVWQV
                     RKQDLRVVCHKSAALAAQTFMLSMSAEGYDTCPMEGFDSRLVKRYMHLPRTAEINMII
                     ACGPGTPEGLYNERIRVPEEDVLFTR"
     gene            complement(3128..5662)
                     /locus_tag="PK742_03255"
     CDS             complement(3128..5662)
                     /locus_tag="PK742_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU75635.1"
                     /translation="MNRLLLMIALLCPLGLSAQTPKPNIVFFFLDDLNDFVEGYEGHP
                     QVETPNIAALSEHGAVFLNTFANSTKCMPSRISMFSGKEVAYTQVEKNVMCEPFRDFF
                     NEAHGNPDVFTFAEVLKDSAGYFTYGINKINHCFATHPDYDATAPDDCSRGLSWNKYF
                     YLDGGEGAEVTLWVAENVGLTPGLNWAKVADSMETKMLDYKLVDSALDFIDNYDADPS
                     VACNKPVFMMMGFRKPHLPWYIPEHYFSDFYLDDVYEIPFPYPYNNPETAFPYNGIKL
                     TPQPDPIWSDYFALPADGLAQIMAEEEHVQPAVDSAYLFQLPLPAIDTGLSPEERKSV
                     MAFSYQANATMAYLASAKFIDAQIGRMLDSMKINYPDFYNNTVFIFSSDHGYTLGEKR
                     SWQKGTLWETDIRVPLVIFDARSPLPTKRVVSNVTSLLDIFPTLLDYAGSPYPMIDGE
                     TPYLDGESLLPYIANPNLTSERAVVTGLRSKTVDCNCFPQWSVRTDQFHYIYYTSNNE
                     NIATPGCQEGEGFHEEELYEIGRFREHDPNEWDNLIDDPDYDPVLQYMRSLLPDSLNY
                     QKDLYKALMQVKSPGCLPSRMETVKIIPQLLNSNGVPVTGPALSNLTFTWTNNLTGHT
                     FSSKNLNFPLSLVPETIFEASEEILFYLHVTDNTSGQLVAFNLAHVYINPDLTPVVDY
                     SLSTNGSEIFIDDLVIEGQRNSTSWRIIGQYQGAEMPSPFTASSSGTYNYKLSVSYGN
                     KPSCVVQANSVITLLETPERISAELPLLQVYPNPAGNSCLIYLADENYSGLIRVYSGL
                     GTPVYEAQITADTPLRMQTAQLPAGVYTIRLEGRADVLPVRLVVAH"
     gene            5720..6205
                     /locus_tag="PK742_03260"
     CDS             5720..6205
                     /locus_tag="PK742_03260"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQU75636.1"
                     /translation="MRYAEMGEKTGWYYLSFSPDDIALLCPGRRQAFRVRGRLNQLTI
                     RQQALIPVGDGHFIMALNADLRKQLGVRLGYEVDIHLEHDESEQQIAEDLRVCLDDEP
                     AAAIFFNSLSRSHQMYFSRWIDSAKTEATRTKRIAMTVQAAARKLGYPEMIRENKKNR
                     E"
     gene            complement(6206..7216)
                     /locus_tag="PK742_03265"
     CDS             complement(6206..7216)
                     /locus_tag="PK742_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU75637.1"
                     /translation="MKRIATVIILVCTLGSAKAQDVHFSQFGAAPMLLNPALSGLSSC
                     TYRAALNYRNQWASIVGPSSYQTYAGSFDIGLFRQAFNYSMLGLGLMVFNDVSGDGAL
                     TNLTMMGSLAYHQNMGGRGAHYLSVGIQGGLVQKSVDYTNLVFESMIGQNGVDPNLPS
                     GEYGSDAFSYFDLNAGVNWRSRIGNNFAFQFGAAYHHLGEPTESFYSQVDNRLNARYS
                     GYGSVKLGFDKIVIIPSAIYMLQTQSSNEEITTGATVGFSLDKGASFLYLGGHYRLDD
                     AVIPSIGFDYNNIQFGLSYDINISDLSAVSAYKGGIELSLIYVGCLTKERKYTIECPR
                     FM"
     gene            complement(7312..9837)
                     /locus_tag="PK742_03270"
     CDS             complement(7312..9837)
                     /locus_tag="PK742_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU75638.1"
                     /translation="MKITLLAVTVLVFCVAGNIHAQIPEKPNILFIIVDDLNDYSGLY
                     ESHPQISTPTIDSLAATGLTFLNGYASNPGCAPSRTSMLSGKSALYTQVFVNGDYDNK
                     FRDNFTAEKNNEVVFTLPEILKDSGGYYTFAINKVFHSPTQNDFDKTTADACSKNLSW
                     NRMINFDDPDELKDEMETYSFMNHFDFGAIPDDLETQLVDYLAANTAIAFLNEVADGI
                     AETCDRPFFLAVGFKRPHSDRYIPAHYFPPYYQNQPESFTTPYMYNLPYNGYPYSGVV
                     MPPQPPEGEYADFYSFPEDGVANTLALSGDYRTPRELYLAGLADYPVEDSTLSEEEKE
                     DITIHSLNAGYVMGYISAVQYIDQQIGRVISTVASDPVLSENTIIILISDHGYALGEK
                     NHYGKWGLWECINRVPVIFSGRGIPADRTGNTITLLDIFPTLLEMTGTNPPLNPDGSP
                     YLDGHSIMPVLEKPDAILTRPALSIHKRGSGVGSCYPHYSVRTDRYHLISYRWNNDGS
                     LGTGVCDDTKYIYENELYDIGANREVDPEEWNNLAGDPEYSGLVNYLSQFFPDSSLYN
                     KPVHGVSILSKNLPCAANHTTKVRLRSRLYSPEGDLITPSDPSVAYRWTHSLGNDTIT
                     TPQLNFNLNTIPAAVFDTLDHIIFYLEILDPETGAMLAFDQFRVFTDDAFEPEAIADF
                     HLTTTDNTAYVTDLELGASVTSRLWDIDGVYTTATPVPAPYTATTVGMHHITLHAGYG
                     NGCTRTVNRNFAVSELRMGEPQEGAILYPNPGKNTCWVATDAEASDIRLVDMAGREQA
                     MTWAPAGDGLYQIRAEGLAAGVYLVVITRQDGIQTISWFVSGR"
     gene            complement(9841..12411)
                     /locus_tag="PK742_03275"
     CDS             complement(9841..12411)
                     /locus_tag="PK742_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU75639.1"
                     /translation="MKTLILFFTYLSVVPVVIAQTGDKPNILFVVVDDMNDWVEGFDG
                     HPQTRTPVMSGLADMGTLFYNAYASAPQCGPSRTSFMTGKDPHYSGVYDNTKLKCASL
                     SSNYTEEEGNETYYTLPGILKDSGGYYTYNIGKVFHCWKDYIDFDSDTPDPCARALSW
                     NDYYFYDDDGAADSLFIDLFFDGLGPFDWGRVDSIYEPLTADYIQADSAISFINRVGS
                     GSGTCDRPFFLALGIKKPHFPLYIPQQYFMNEFYMDDVYEEPFHIAFNFPEGTTPYNG
                     IVLPDQTSPSRYLDYFALPEDGMARTICDKQEKNFEEYTLYLSPLPVVSDTLTDSLNR
                     LFLEQSKRANFLNAYLAGIHFADAMLGRVMEALEAYPEMYNNTIVVVFGDHGFSLSEK
                     RHWQKSTLWETDVRTSLLIADLRDPEPAVSYRMAGFVDLMPTLLDLAGVDLPYFPDGN
                     LYPDGRSLTPFMHDGFKKTDRPVLSTIANANGKPASCYPHHSVRNNRFHFIHYMVQSG
                     TNVMDCDSVPGALEEELYDIGYEKETDPNEWNNLAYDPNYAPVITFLKQWIPGHPMYL
                     QEAYYLELTPDDSAVCLYDNDTNISFSCQVYDSLGTLTDPGTLAHHVLEWTNNYDETV
                     QYGSSYMLNAGSIPDAVMLDENAVYLYAQLRDTTNQNITGFQLYTIHLHSEDAPTMSF
                     SHAVDDHTVSISGYSLTGSAIDPVWNFGDGTVLHIALPGAHTYSEDGYYQLTHTLRYG
                     NDCMLIQTDTVIIGDTIPEDTLITDTTLLILDLMENQFEVNPNPASSSIQVSWPQPSG
                     PGTFMIGNSSGVLISKRSYPEGMPESTIWHLEQLPAGLYFIIWQTEQHTDLRRIAVTH
                     "
     gene            12449..13231
                     /locus_tag="PK742_03280"
     CDS             12449..13231
                     /locus_tag="PK742_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQU75640.1"
                     /translation="MNVLCIMQIATYNLNGIRSALNKGWMNWMQETSFDVVCIQELKA
                     QNEQIDLEAFAQAGYHVYTETAEKKGYSGVAIFSKQKPDHVEYGCGNQEFDKEGRVIR
                     ADYGDLSVFSIYFPSGTTGDVRQSVKYVFLDYIYEYLMTLQKTRKKLVVCGDYNIAHR
                     EIDIHDPRGNKNSSGFLPEERAWMDKWFGSGYTDSLRVFSDAPHQYTWWSFRANARAN
                     NKGWRIDYISVTDNLKDKLRSATILPDALHSDHCPMVIELDT"
     gene            complement(13232..>15709)
                     /locus_tag="PK742_03285"
     CDS             complement(13232..>15709)
                     /locus_tag="PK742_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU75641.1"
                     /translation="ALHCYPVLLPAQERPNVIVILLDDLNDYASLYDGHPQSPTPAIA
                     AIEDRGITFLNAHASAPKCNPSRTSFLTGKDCAYTQVYNNMACHPFRSYFNEADGNGV
                     VYTLPEIMKDSGGYFTFGINKINHCFDTWPDLDTTAAAPCNREWSWNRYSLFVEGESE
                     VVKDWGNDHQLGFGGLAWSIIPDSLESYMYDYQAVDTLQQFLHEALTDPSTVCNKPLF
                     VMIGLRRPHSPWYIPEKYYAPYYLDDYYENPLIPSYNHPQDQFPYNGVVMPPQPETLF
                     GDYYSLPDTSLARYVIDQENNYADFTNRIYQLDPLPPVGAGLSEDERFQILVNSYMAN
                     AVIAYLAATSFVDAQIGRIMTYLEEEQPAFLSNTIIVIASDHGFSLGEKSHWKKSTLW
                     ETDLRAPFIVADYREERNGVSGINASLLDLFPTLCGMTGVPEPKFPDGSRYLDGMDLG
                     PFMAEPNLYAERPALAAYEEHSNLECSCFPMYSVRNDRFHLHRFASNGPVFLDTDCNE
                     EENYYEYLLYDIGVDRQTDPNEWNDLAADPDFRPVMDYLLQWLPGHPLYLQKTWKPRI
                     QFETDCLVADTTILVADMVLTDTNGLETLLPDTLTARWFTNMSADTLTGAEVIFPAAW
                     NIDALLTGEPLLLFLELTDTNGIVRGLDMQYVFTTGHGLPDVELSVYPSGLQVFLEDI
                     HYSGSPSDAYWVMGNGDTLFSMFPSSYTYSSDGDYTAEVHLLYGNDSSCEVVKTFDWS
                     LTDIGFSDLFLVYPNPSKGMFTLTSDEIVEVQQILLFDMQGRQLRNWTADKGSLWQLD
                     AGDLPAGVYLLQINTEKEEYSLPLMLE"
BASE COUNT         4057 a         3631 c         4052 g         3969 t
ORIGIN      
        1 tatgatgaaa ttacccatat ttccatcgtt gttcaggagt tcatcggcag gaatgagttc
       61 atataatgca gtaacggttt ggcccatgcc catctcaccg gcatcggttt tccgtttatc
      121 aaattcctta tcggtcagcg cacgattctc atatccgatg agtcgccagg ctttcacata
      181 gcgctcatca aaggtggcct ccagtttcac atcacgtgca acagttacca gcgtgccgcc
      241 catttcttcc accagcagtt tgcgtgcttc ttctatctgg tctatgtagc cataattacc
      301 gttgccatta tcggccagca attccagtct gccatcctgc aggttgtcca tcccgaaacc
      361 gagcacggtc aggtaaactc cgttcttccg ttgagaacgc actagttgaa gcagtgcatc
      421 atcgctggtg ggtcctacat tgaagtcgcc atcggaacaa agaatgatcc tgttatttcc
      481 gttgcgaatg aaatgctttc cggcaatatc ataggctttt tccagccccg gagcaccggc
      541 ggtagaacca tcggcataca tgcctgccaa tgcccgcaga atggcttcct tgtcgcttcc
      601 gggaaggtct tccgccacca cctccaccgt ttcccgatag gtcacgatgc tcactctgtc
      661 ttgcggacga agctgtctga ccagggtacg catgcttgat ttagcaaggg gcagtttatc
      721 gctggtagcc atgctgccac ttacatccat cagaaacacg agatgtgaag gaggcaggtc
      781 atcctggaag acttctctgg tttgcagacc gatatgcagc agcagatgat cctggttcca
      841 ggggcaagct gcagattccg tataaacaga gatatcagat ccatccggtt tcgggtattc
      901 atagtcgaaa taattgagca tttcttctat acgaactgct ccctgtggcg gaatgacccg
      961 gtgcatgatc atccgcctga caatggtata gaaagcattg tccacatcag ccgagaaagt
     1021 ggagttggct ttttcttttg tgagtacaaa atcctgttcg ggtatgaagg gatagtactg
     1081 tcctttgaat tcttctttga cttcatcgct tttcttctcc accggcaaca caatggccag
     1141 ggcaatggga ttggtaacag gatcgggtct gatgctgaga tcctgtgcaa tgatgtcttt
     1201 atctccgatg gtcacgttca aagtagtgat catgccggcc tgtatctcta ccccggtaac
     1261 ggtcctgttc tccagttgtc ttccttttac cgcgaaggct acatcgaagg tgttcggata
     1321 cagcttttca tacatataat tgccgttggc gctggtaccc tgggcagaca acagctggcc
     1381 gtcctgcatg atgatgacat tgacattggg tagcggtaag ccggtagtgg catcggtcac
     1441 tttaccctgt aaggtgccta cattggtctg agcgaaggaa acggcactca gaaaaagagc
     1501 accggcaagt aattgtgtgc gcatggtgtg gttgttggga aagtttataa cagatgcata
     1561 cggggagata ttgtataagt ggcagtgctt ctgctcccat aggtgcgggt tttaacccgc
     1621 acctatggaa gaaaaaataa tcacgaggtg caggcataca gatgtttgag agtgtggggt
     1681 gaaccctgca acaggcggga gagaatggtg agcagttagt aattcaataa tgcgcatttc
     1741 tcactactca ctcgttgccg caggaaaggt ggacttctct cttgtacgtc atcaaagcgc
     1801 ttgtgtaaaa agttaccatt cgttaatgtt tatggatttc cgattgggaa tttatccatt
     1861 ataaaaaaac acacatgaaa cgaaaacatc ttttgtgggc tgtgcttccg ctcatggcac
     1921 tgatcaccgg ttgtccggta ggcgttgatc atccgctggg tgaaccggga aaggaaaaac
     1981 tggatgaaca gctcataggt acctgggtgc agccggatac ggcgatggaa gccacgagga
     2041 tcgcgatcga aaagatcgac aagcactcct ttaagctgac ggtgctggaa gagggcggca
     2101 tgttcatgga agaaactgac ttcttccggg gctggtgcac aaaagtggat ggctacacct
     2161 tcgtctatct acaggatctg aacaatcctg caggacaata cctgacctac tgttacagaa
     2221 tggacggcga cgatctttat acctatgata tgactcttgc agtgggtggc atagatgcgg
     2281 taaccagtac agaggcattc aggaaggaag ttagtgcctc cctgaagctg gagggcagcc
     2341 tgacaagtga agtaaagtgg tccagacaat gagcgggcat gcagtaagtt tgcagcatgc
     2401 aaaatgaagc aacggtcttt gatgacattg ttcagcgcag aagatccatg cgcatctttt
     2461 cagcagatgc acccatggat gaaggtgctg tacaacgcag cctgcaacgg gccttgctgg
     2521 cacccaacag cagcaatatg caggtgtggg aattctaccg ggtcagtgat ccggagaaat
     2581 tacaggtgct ggcgaagtac tgtatgggtc aacgtgctgc caccaccgca cgtgaactgg
     2641 tactggtggt ggtgcgtcgt gatctgtgga aaaaacgggc tgactatatg gtggagttca
     2701 atgaacagta ctatgcagac aagccggagt tgcagcatcg actgaaacgc attaaaaaat
     2761 attacaggcg tttgatgccc atgtattact tccaggactg gttcggcatc tgggggcgga
     2821 tgcgacaact gatcgtgttc tttgcatcct tccgccgccc ggtggtctgg caggtgcgca
     2881 agcaagacct gcgtgttgtt tgccacaaga gcgccgcact ggcagcacag accttcatgc
     2941 tgagtatgag cgccgaaggc tatgacacct gtccgatgga aggttttgat tctcggctgg
     3001 tcaagcgata tatgcatctt ccccgtactg cagagatcaa catgatcatc gcctgcggtc
     3061 cgggcacacc ggaaggtctc tacaatgaac ggatacgcgt tccggaagag gatgtattgt
     3121 tcacccgtta gtgagcaacc accagtctta ccggcaatac atcagcacgt ccttccaggc
     3181 gtatggtgta cacaccggca ggcagctgag cagtttgcat gcgcaggggt gtatctgctg
     3241 tgatttgagc ttcatacacc ggagtaccca gaccggaata gaccctgatc aaaccgctgt
     3301 agttctcatc agcaagatag atcagacaag agttgccggc cgggttagga tacacctgaa
     3361 gcaaaggcag ttcagctgag atacgttcag gggtttctaa cagagtgatg acactgttgg
     3421 cctggaccac acaggagggt ttattgccgt aagacacact taatttataa ttataagttc
     3481 cggatgaact tgcagtgaat ggagagggca tttcggctcc ctgatattgt ccgataatgc
     3541 gccagctggt gctgttgcgt tgtccttcta tgaccaggtc atcaatgaat atctctgaac
     3601 cattggtgct gaggctgtag tcaaccacgg gtgtaagatc cggattgata tacacgtgtg
     3661 ccagattgaa tgccaccagc tgaccggatg tattatcagt tacatgcaga taaaaaagga
     3721 tctcttcact ggcctcaaag atcgtttcag gtaccaggct taaaggaaag ttgagattct
     3781 ttgaactgaa ggtatgaccg gtcaggttat tggtccaggt aaatgtaagg ttggacagag
     3841 ccggaccggt aacaggtact ccattggaat tcagcaactg tggaatgatc ttgaccgttt
     3901 ccatgcggga tggtaaacag cccggagatt tgacctgcat caaagcttta tacagatctt
     3961 tttgatagtt caaactgtca ggcagtagtg aacgcatgta ttgaagcaca ggatcataat
     4021 caggatcgtc tatcaggtta tcccactcat tggggtcatg ctctctgaaa cgacctatct
     4081 cgtagagttc ttcttcatgg aatccttcac cttcctgaca tcccggtgtg gcaatatttt
     4141 cattattgga agtatagtag atgtagtgga actggtctgt gcgcacactc cactgcggaa
     4201 agcaattaca gtcaacggtc ttggaacgaa gtccggttac tactgcccgc tctgacgtga
     4261 ggttgggatt ggcgatatac ggcaacagac tttcaccatc cagatagggt gtttcaccat
     4321 ctatcatcgg gtagggtgaa ccggcataat caagcagggt cgggaaaata tccagcagtg
     4381 aggtcacatt actgaccact cttttggttg gcagaggtga acgggcgtcg aagatgacca
     4441 gcggaacccg aatatcggtt tcccacaaag tgcctttctg ccagctgcgt ttttcaccaa
     4501 gggtatagcc gtggtcactg gagaaaatga acaccgtatt attgtagaaa tccggataat
     4561 tgatcttcat actgtccagc attctgccaa tctgtgcgtc tatgaatttt gcagaggcca
     4621 gataagccat ggtggcattt gcctgatagg aaaatgccat gacagacttg cgttcttcag
     4681 ggcttagtcc ggtgtctatg gctggcagtg gtaactgaaa cagataagcg ctgtcaacgg
     4741 caggttgtac atgctcttct tctgccatga tctgagccag cccgtcggcc ggcagggcaa
     4801 aatagtcaga ccagatcgga tccggttgcg gcgtcagctt gatgccgttg taaggaaagg
     4861 cggtttcagg gttattgtaa ggatacggaa atggtatctc atagacatca tccagataaa
     4921 agtcagagaa ataatgctcc ggaatatacc agggcagatg gggtttacgg aagcccatca
     4981 tcatgaaaac gggcttatta caggccaccg atggatcggc gtcatagtta tcaataaaat
     5041 cgagggctga atcaaccaac ttgtaatcca gcatctttgt ttccatagaa tctgccactt
     5101 tagcccagtt caggccgggg gtaagtccaa cattttcagc tacccaaagg gtcacttctg
     5161 ctccttcacc accatccagg taaaagtatt tgttccatga caggccacgg ctgcaatcat
     5221 cgggtgccgt agcatcgtag tcgggatgtg tggcaaagca gtgattgatc ttgttaatgc
     5281 cataagtgaa ataacctgca ctgtctttca gcacttcagc aaaagtgaac acatcaggat
     5341 ttccgtgtgc ttcattgaaa aagtcgcgga agggttcgca catcacattc ttttccacct
     5401 gggtataggc tacctcttta ccgctgaaca tgctgatgcg ggaaggcatg catttggtgc
     5461 tgttggcaaa tgtgttcaga aaaacggcac cgtgttcaga cagggctgct atgttggggg
     5521 tttccacctg aggatgaccc tcgtagcctt ctacaaaatc attcagatca tctaaaaaga
     5581 agaatacgat atttggttta ggtgtttgtg cagataaccc taacggacac agcaatgcga
     5641 tcatgaggag gagcctgttc atacaacaaa actaataagg ttacctttaa tcgtgccttt
     5701 agcatgtcat aaaagggttt tgcgatacgc agagatggga gaaaagaccg gatggtatta
     5761 cctgtctttc agcccggatg atatagctct gttgtgtccg ggaagaaggc aggcttttcg
     5821 tgtcagggga cgtttgaatc aactgacgat caggcaacag gctctgattc cggtgggcga
     5881 tggtcacttc attatggcac tgaacgctga cctccgcaaa cagcttggcg tgagattggg
     5941 atatgaggtt gacattcatc tggaacacga tgaatcagag cagcagatcg ctgaagatct
     6001 gcgggtctgt cttgatgatg aaccagcagc agcgatattt ttcaattcgc tttccagatc
     6061 tcatcagatg tatttttcac gttggataga cagtgccaaa acggaagcta cccgtaccaa
     6121 acggatcgcc atgacggtcc aagccgctgc acggaagctg ggatacccgg agatgatcag
     6181 agagaacaaa aaaaaccggg aatgatcaca taaagcgagg acactcaatg gtgtacttgc
     6241 gctctttggt cagacagcca acatagataa gagataattc aatacctcct ttataggcac
     6301 taaccgcact caggtctgag atgttgatgt cataactcag gccaaactga atgttgttgt
     6361 aatcaaatcc aatgctggga atgaccgcat catccaggcg ataatgacca cccagataga
     6421 ggaaggaggc gcccttgtca agcgaaaaac caacggtagc accggtggtg atctcttcgt
     6481 tgctgctttg ggtttgcaac atatagatgg ccgaaggaat gatcacgatc ttgtcaaagc
     6541 ccagtttgac actaccatat ccggaataac gggcattgag tctgttgtca acctggctgt
     6601 agaagctttc ggtaggctca cccaaatggt ggtaagcagc cccgaactga aaggcaaaat
     6661 tgttacctat gcgtgaacgc cagttgacac cggcattgag gtcgaaataa gagaaagcat
     6721 cagagccgta ttcgccgctg gggaggttgg gatccactcc attctggccg atcatacttt
     6781 cgaaaacgag gttggtataa tccacgcttt tctgcaccag tccgccctga atacctacag
     6841 agagataatg cgcacctctt ccgcccatgt tctggtggta ggccagactt cccatcatgg
     6901 tgaggttggt cagggctcca tcgccgctta catcattgaa taccatcagc cccaggccca
     6961 gcatggaata attgaatgcc tggcggaaca atccgatatc aaatgaaccg gcataggtct
     7021 ggtaagaaga aggacccacg atactggccc actggtttct gtagttgagt gccgcccggt
     7081 aggtacaact ggacaggcct gacaatgcag gattcagcaa cattggagct gccccaaact
     7141 gcgaaaaatg cacatcctgt gctttagcag agcccagggt gcaaaccagg atgatgaccg
     7201 tagcgatcct tttcatacgc ttgaataacc gtgtaactat caaaattagt ataaatccga
     7261 gaaatcaaca caaaataaac acccatgaaa cgtcagacca atgtcaaacg cttatcttcc
     7321 tgaaacaaac cagctgattg tttgtatgcc gtcctggcgg gtgatgacca ccaggtacac
     7381 acctgcggcc aacccctctg ccctgatctg atacagacca tcgccggccg gagcccatgt
     7441 cattgcctgt tctcttccgg ccatgtccac caaccgtatg tcgcttgctt cggcatctgt
     7501 tgccacccag caggtgtttt ttcccgggtt aggatataaa atcgccccct cttggggttc
     7561 gcccatccga agttccgaaa cagcaaaatt cctgttgacc gttcgggtac aaccatttcc
     7621 gtatccggca tgcagggtaa tatgatgcat acccacagtg gtggcggtgt agggagccgg
     7681 aacaggggta gccgtggtgt acacaccatc tatatcccaa agccgtgagg taacagaagc
     7741 gcccagctcc agatcggtca cataggccgt attgtctgtt gtggtcaggt gaaaatctgc
     7801 aatagcttcc ggttcgaaag catcgtctgt gaacacccgg aactgatcaa aggccagcat
     7861 tgctcctgtt tccgggtcga gtatctccag gtagaagatg atgtggtcaa gggtatcgaa
     7921 gacagctgcc ggtattgtat tcaggttgaa gttcagttgt ggtgtcgtga tggtgtcatt
     7981 gcccagcgaa tgggtccacc ggtaagctac agaaggatca gagggtgtta tcagatcacc
     8041 ttccggcgaa tacagcctgg agcgaagtct gaccttggta gtatggtttg ctgcgcatgg
     8101 caggttcttg gaaaggatac ttacaccatg aacgggttta ttgtacagag agctgtcagg
     8161 aaaaaactgc gacaaataat tcaccagccc ggaatattca ggatctcctg ccaggttgtt
     8221 ccattcttcc ggatcgactt cgcgattggc accaatgtca tacaattcat tttcgtatat
     8281 atatttagtg tcatcacaaa caccggtacc caatgatccg tcattgttcc accggtagct
     8341 gatcagatga tagcggtctg ttctgactga gtagtgcgga tagcagcttc ctacgccaga
     8401 tcctctcttg tggatggaaa gtgccggccg ggtcagtatt gcatcaggtt tctccagaac
     8461 aggcatgatg gaatgaccgt ccaggtaggg cgatccatcc ggattgagcg gagggttggt
     8521 tcctgtcatc tccagcaggg tcgggaaaat atccagcagg gtgatggtgt taccggtgcg
     8581 gtctgccggt attccccgac ctgaaaagat aaccggaaca cggttgatgc attcccatag
     8641 gccccacttg ccatagtggt ttttttctcc cagggcatag ccatggtcac tgatcagtat
     8701 gatgatggtg ttctctgaca acaccggatc agaggctacc gtactgatca cccggcctat
     8761 ctgttgatcg atgtattgca cggccgatat ataccccatc acatagccgg cgttcagcga
     8821 gtgtatggtg atgtcttctt tctcttcttc tgacaatgta gaatcttcca cagggtaatc
     8881 ggcaagaccg gccagataca gctctctggg cgtccggtaa tcaccgctca gtgccagggt
     8941 attggccaca ccatcttcgg gaaaggaata aaaatcagca tattctcctt cgggcggttg
     9001 tggtggcatg accactcctg aatagggata accgttatag gggaggttat acatataggg
     9061 ggtggtgaat gattcaggtt ggttctggta gtatggagga aaataatgcg caggaatata
     9121 cctgtcgctg tgcgggcgtt tgaaaccaac tgccagaaag aaaggtcggt cgcaggtttc
     9181 agcaattccg tctgctacct cgttcagaaa ggcgatggca gtattagctg ccaggtagtc
     9241 cacgagttgc gtttccaggt catcagggat ggctccaaag tcgaaatggt tcatgaacga
     9301 ataggtctcc atttcatcct tcagctcgtc aggatcgtcg aaattgatca tcctgttcca
     9361 gctgaggttt ttagagcagg catctgcagt agttttatca aagtcgttct gagtaggtga
     9421 atgaaagacc ttgttgatgg caaatgtgta gtatccgccg ctgtctttga gaatttcagg
     9481 cagggtaaac accacctcat tattcttttc agccgtgaag ttgtcgcgaa atttattgtc
     9541 gtagtcacca tttacaaata cctgtgtgta cagagcactc ttgccgctca gcatgctggt
     9601 tcgtgaaggg gcacaacccg gattgctggc atagccgttc agaaaggtca gtccggtggc
     9661 agccaggctg tcaatggttg gggtagagat ctgcggatga gattcataca agccggaata
     9721 atcattgagg tcatcaacta ttatgaacag aatgttcggc ttttcaggta tctgcgcatg
     9781 tatgtttccc gccacacaga agaccagaac tgttactgca agcaaggtta ttttcatgga
     9841 tcaatgggtt acggcaattc ttcgcagatc agtatgctgc tctgtttgcc atatgataaa
     9901 atagagtccg gcaggcaatt gctccaggtg ccagatagtg ctttcaggca tgccttccgg
     9961 atagcttcgt ttgctgatca aaacaccact gctgttgcct atcatgaagg tgcctggtcc
    10021 tgaaggctgt ggccagctta cctgaatact ggaagaggca ggatttggat taacctcaaa
    10081 ctgattttcc atcagatcca ggattagtag tgtggtatct gtgatgaggg tatcttccgg
    10141 tatcgtatca ccaataatga ccgtgtcagt ttgtatgagc atacagtcgt ttccgtaacg
    10201 aagtgtatgg gtgagctgat agtaaccatc ttcagaatag gtgtgtgctc ccggcaaggc
    10261 gatgtgcagc actgtaccat ctccaaagtt ccagaccgga tcgatggctg atcctgtaag
    10321 gctgtaaccg gaaatggaaa cggtatggtc atctaccgcg tgcgaaaaag acatggtggg
    10381 tgcatcttcg ctgtgcaggt gtatggtgta cagctgaaat cctgtaatgt tctgattggt
    10441 agtatctctg agctgtgcat agagataaac tgcattctca tccaacatca cagcgtcggg
    10501 tatggatcct gcattcaaca tatagctgct gccatactgc accgtttcat catagttgtt
    10561 ggtccactca agcacatgat gggccagcgt tccagggtct gtcagggtgc ccagagaatc
    10621 atagacctga caactgaaac tgatattggt gtcattgtca tacagacaaa cagcgctgtc
    10681 atctggagta agctccagat agtatgcctc ctgcaggtac atgggatgcc cgggaatcca
    10741 ttgtttcaga aatgttatga caggcgcata gttggggtca tatgccagat tattccattc
    10801 atttgggtcc gtttcttttt cataaccgat gtcatacagt tcttcttcca gtgctcccgg
    10861 aacactgtcg cagtccatta cattggtgcc tgactgaacc atgtagtgaa tgaaatggaa
    10921 acggttgttg cgcacgctgt ggtgaggata acagcttgca ggtttgccgt ttgcattggc
    10981 gatggttgac aataccggac ggtctgtttt tttaaaacca tcgtgcatga aaggtgtcag
    11041 gctgcggcca tccggataca ggttgccatc gggaaagtag ggcaggtcaa ctccagccag
    11101 gtccagcagg gtgggcatga gatcaacaaa accggccatc cggtaactca ctgcgggttc
    11161 cggatcacga aggtctgcaa tgagcaggga agttctgaca tcggtttccc agagggtcga
    11221 tttttgccaa tgccgttttt cactgagaga gaaaccatga tcgccaaaga ccaccacgat
    11281 ggtgttgtta tacatttcgg gataggcttc cagggcttcc atcacacggc ccagcatggc
    11341 atcagcaaaa tggatcccgg ccagataggc attcagaaaa ttggcccgtt tagactgttc
    11401 caggaatagc ctgttgagcg aatcggtaag ggtatctgaa accacaggaa ggggtgagag
    11461 atacaaggtg tattcttcaa aattcttttc ctgcttgtca cagatggtcc gtgccatgcc
    11521 atcttcgggc agggcgaaat agtccagata gcgggatggt gaagtttgat caggaagcac
    11581 aatgccattg tagggcgtgg tgccttccgg aaaattaaag gcgatgtgga agggttcttc
    11641 atagacatca tccatataga actcattcat aaaatactgc tgtggtatat acagcggaaa
    11701 gtgtggcttt ttgataccca gtgccagaaa gaacgggcga tcgcaggtac cactgccgct
    11761 gcctacccgg ttgatgaacg atatggctga gtctgcctgg atgtaatcgg ccgtaagggg
    11821 ttcatatatg gaatccacac gaccccagtc aaacggcccc agcccgtcaa agaacagatc
    11881 aatgaacaga gaatctgcag caccatcatc atcatagaaa tagtaatcat tccagctcag
    11941 cgcccgggca cacggatcag gtgtgtcact gtcaaaatct atgtagtctt tccagcaatg
    12001 aaataccttg ccgatattgt aggtgtagta gccaccactg tctttcaata tgccgggaag
    12061 ggtataataa gtttcattgc cttcttcttc tgtatagtta gaactgagag aggcgcattt
    12121 gagcttcgta ttatcataca ctcccgaata gtgcggatcc ttcccggtca taaaactggt
    12181 acgtgatggc ccgcattgtg gtgcgcttgc ataggcgttg taaaataaag tgcccatatc
    12241 tgccaggcca gacatgaccg gagtgcgggt ttgcgggtgc ccgtcaaaac cctctaccca
    12301 gtcattcatg tcatcgacca caacaaatag tatgttgggc ttgtcgccgg tttgagctat
    12361 gacaacagga actaccgata agtaagtaaa aaagagaatc aatgttttca tgactatcgg
    12421 gaatttaata ttagtcaatt tctgtcacat gaacgtactt tgcatcatgc agatcgctac
    12481 atataatctt aatggaattc gttcagcact aaacaaaggt tggatgaact ggatgcagga
    12541 aacctctttt gacgtggtat gtattcagga gttaaaggca cagaatgagc agatagatct
    12601 ggaagcattt gcacaggccg gataccatgt gtacacagaa acggcagaga aaaagggata
    12661 tagtggcgtg gccattttca gcaagcagaa gccggatcat gtggaatatg gctgtggcaa
    12721 tcaggagttt gataaggaag gacgggtcat acgtgcagac tatggcgatc tgagtgtgtt
    12781 cagcatatat ttcccttcgg gtaccacagg tgatgtaagg cagtcggtca aatatgtatt
    12841 tcttgattat atatatgaat atttaatgac actgcagaaa accaggaaga aactggtggt
    12901 ttgcggcgat tataatatcg ctcatcgcga gatcgatatc catgatcccc ggggaaacaa
    12961 aaactccagt gggtttctgc ccgaagaaag agcctggatg gataagtggt tcggttcggg
    13021 ctatacagat tcgttgcggg tattttctga tgccccgcat caatatacct ggtggagctt
    13081 ccgggcaaat gccagggcga acaacaaagg atggcgcatt gattacatct cggtaacaga
    13141 caacctaaaa gacaaactaa gatcggcaac cattctgccc gatgcgctgc acagtgatca
    13201 ttgcccgatg gtcatagaac tggatacctg atcattccag cattaatgga agggaatatt
    13261 cctctttttc agtattgatc tgcaggagat acactccggc aggcagatcg ccggcatcga
    13321 gctgccacag gctgcccttg tctgccgtcc agtttcgcaa ctgccttccc tgcatatcga
    13381 agagcaggat ctgctgtacc tccacaatct catctgaggt cagggtgaac atgcctttag
    13441 aggggttggg gtacaccagg aacagatcac tgaaacctat atctgtgagt gaccagtcaa
    13501 atgtttttac cacctcgcaa ctgctgtcat ttccatacaa caggtgtacc tctgcagtat
    13561 agtctccgtc actggagtag gtataagaag acgggaacat gctgaacagg gtatcgccgt
    13621 tgcccataac ccagtaagcg tctgaaggag aaccggaata atggatatct tccagaaata
    13681 cctgcagacc agaagggtac acagacagct ctacatccgg taatccatga ccggtggtaa
    13741 acacatactg catatcaagg ccgcgaacaa tgccattggt atctgtcagt tccaggaaca
    13801 gtaatagcgg ctcaccagtg agcagggcat caatattcca tgcagcagga aaaatcacct
    13861 ctgcacctgt cagggtatct gcagacatgt ttgtgaacca cctggccgtc agggtgtcag
    13921 gtaacaaggt ttcaagtccg ttggtatctg tcaataccat atcggcaacc aaaatggtgg
    13981 tatcagctac cagacaatct gtttcaaact gaatgcgtgg cttccatgtt ttttgcagat
    14041 agagcggatg accgggcagc cattgcagca ggtaatccat gaccggcctg aaatcagggt
    14101 cagctgccag atcgttccat tcattgggat ctgtttgtct gtcaacgcct atatcatata
    14161 gcaaatattc ataatagttt tcctcttcat tgcaatcagt gtccagaaat accggaccat
    14221 tggaagcaaa gcggtgcagg tgaaaacggt cgttgcgcac agaatacatc gggaaacaag
    14281 aacactccag gttgctgtgt tcttcatagg cagccagggc aggacgctct gcatacaggt
    14341 tcggttcagc cataaagggg ccgagatcca taccatccag gtatctgctg ccatcgggga
    14401 acttcggttc cggtacaccg gtcatgccac acagggttgg aaagagatcc aggagactgg
    14461 cgtttatacc agacacaccg ttgcgttctt cgcgataatc tgctacaatg aacggcgccc
    14521 gaagatctgt ttcccacagg gtgctttttt tccagtggct tttttctcca agactgaaac
    14581 cgtggtcact ggctatcaca atgatggtat tggacaggaa tgcaggttgc tcttcttcca
    14641 ggtaggtcat gatcctgcct atctgtgcat caacaaatga cgttgctgcc agataggcga
    14701 tcaccgcatt ggccatataa gaattgacca ggatctggaa tcgttcatct tcgctcaatc
    14761 cagctcctac gggaggcaat ggatccagct gatatatcct gttagtaaaa tctgcatagt
    14821 tgttctcctg atcgatcaca taacgggcca ggctggtgtc aggtagcgaa taatagtcgc
    14881 cgaagagcgt ttccggctgt ggaggcatga ccacaccgtt atagggaaac tgatcctgcg
    14941 gatggttata ggaagggatg aggggatttt cgtaataatc gtccagataa tatggtgcgt
    15001 aatacttttc aggaatgtac cagggtgaat gcggtctgcg caaaccgatc atgacgaaca
    15061 ggggtttgtt gcatacggtc gatggatcgg tcagtgcttc atggagaaat tgctgcaagg
    15121 tatccacagc ctggtagtca tacatgtaag attccagact gtcaggaatg atgctccagg
    15181 ccaggccacc aaatcccagc tggtggtcat tgccccagtc cttgaccact tcgctttcac
    15241 cttccacgaa cagactgtac ctgttccacg accattcgcg gttgcagggg gcagcagcag
    15301 tggtatccag gtcgggccag gtatcaaagc aatggttgat cttgttgatg ccaaatgtga
    15361 agtatccgcc gctgtctttc atgatctccg gcaaggtata taccacacca ttgccatctg
    15421 cctcattgaa gtaggagcgg aaaggatggc atgccatgtt gttgtaaacc tgtgtatagg
    15481 cacagtcttt accggtcaga aaggaagttc tggacggatt gcatttgggt gcgctggcat
    15541 gggcgttgag aaaggtgatg cctctgtctt caatagctgc aatagcgggt gtgggcgact
    15601 gtggatgacc atcgtacaaa gaagcatagt cgttcaggtc atccagcaga ataacgataa
    15661 cgttgggccg ttcctgtgcc ggcagcaaaa ccggataaca atgcaaagc
//
LOCUS       DAOMAX010000034        15488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_428_length_15488_cov_4.686710, whole genome shotgun sequence.
ACCESSION   DAOMAX010000034 DAOMAX010000000
VERSION     DAOMAX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15488
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_15488_cov_4.686710"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..111)
                     /locus_tag="PK742_03290"
     tRNA            complement(38..111)
                     /locus_tag="PK742_03290"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Ala,seq:ggc)
     gene            complement(156..4133)
                     /locus_tag="PK742_03295"
     CDS             complement(156..4133)
                     /locus_tag="PK742_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75642.1"
                     /translation="MYKLFAGFCLLLSVSVSAQKTGGVYLPEPDAQTPDWFLPLYADE
                     IPNVFVMDSLFDASHLNGMPEEEHRTIWEFYYQRWRRSVEPFIQADGSVAQVEVSATG
                     TVHAKATGSWTLAGPATTHFDAADDPSQPECPWQVNVYAITADVNNPDRMLAAAETGG
                     LFYSSNKGLAWTLALDNATTYGSVAISPGDPDLMLAGANGSLARSTDGGSSWTTMSLA
                     AGNIHSIAFHPSDPQKVLLATDYGLFRSTDGGLSFAVTDGITSHMYDVYFHTTDPERV
                     IAIRVQSGVIDCMRSNDAGVTFESGFAGVTPVNTSGARMSLTADDPERVYVVALGTLP
                     SVTAPHVYRSDDGGISWELMCIGIESGLTGDDSSPLGMSNGQGFYDLDIVANPLNADE
                     VIVASTTAYRSTNGGSSFLRLGGYGGSFNIHPDIQEMIAIDGDTWITTDGGVNYSTDF
                     FASTANYESRTNGIFGTDFWGFSQGWNADYMAGGRYHNGNTSLSDQYPEGASIRLGGG
                     EAGTGYAMAGREGYFAFSDIGNFQVPLSFDGPRKYFAFGMYPNEDGYGFDASDVEFLP
                     YCYGVVFLGNGNALWRSDNLGASFTTLYDFGAGIGNKVKKFEISRSDPQTIYVATANK
                     LNRSDDGGTSFTNLTLPTGVSTYRMDLAIDAQDPQTVWISSPSNPAGKRVYVTHDGGV
                     SWENLTTTLIDGQGYACVVHHKGSEGGVYVLGNNGAAFYRDDTMADWTDVSDGLPAGN
                     RPIRSFAFYRDSKIRVAGNRGVWERDLEDTYAPIAQPTVDKLVSACALDTFYFEDYSV
                     MDHSGATWSWSFSPAPEFVSNATARNPKVVFGAEGVYDFTLTISNAWGTDTKTETGAI
                     SISGNECGVDTVAGMAIALNGNADPADVSVPPLGITTNHLTISMWFKPDGIQTDYSSL
                     FMLEGSDAAGFNFRPGDNKLGYHWPGGAWWWESGLIVPEGRWSHLAMVVEPDGITLYV
                     NGEGSKHNITVSPTNLENNNVLLGSYKHWGGRYIKGEMDEVAVYDRSMSEEEIQDLMH
                     LTRNNPHPDNLPQDDESLIAYYQFNESVGYFYNKAGGNQALPEGSAVQIASTAPVAGG
                     TFDRVYADTEDPVVFDHTGVTLTWAEVPGTDVVVSRLHARPDVVPGDSLLPDPERYFI
                     VRQFNGAGTASLNAISFTSIGGINDSMTAHPEYVELYNRPYYSEGDSWGDLFATADEA
                     AGSTLAFSPAGDSWLGQFSISADAKAFAGSTALETEVAGGITLYPNPSADGWIHWRTT
                     MNAREGYQVRITDVAGRVVRQFRVNVGLSDQEQHVLISAPGTYHLELIFTDGSRQVLP
                     FVVAY"
     gene            complement(4156..5268)
                     /gene="lpxB"
                     /locus_tag="PK742_03300"
     CDS             complement(4156..5268)
                     /gene="lpxB"
                     /locus_tag="PK742_03300"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQU75643.1"
                     /translation="MKYYILAGEASGDLHAANLVKALREQDTQAQFRGFGGKRMQAEG
                     VQLDRHIDQLAFMGFTEVLLHLRKISANFKTAKQSISNFQPDVVICIDYPGFNLRMLP
                     FIKSLGIRTVYYISPQLWAWKAGRVKTIKQYVDHMLVILPFEKAFYEGYDYQVEYVGH
                     PLLDEISDMDAEPDFLPKNSLPDGSLVALLPGSRKQELKRSMPVLLEVANERADLQFV
                     IAGAPSLDAAAYASFDLPANVHLVHGQTYALLQHVKAALVTSGTATLETALFGVPQVV
                     CYRGSTLSYWIARRLVKVRFISLVNLIMNSELVPERIQSSFNRRQLLRDLDDLLQPEK
                     AAIMQQGYMELRLKLGGQGASSRAAAIITDALHKAL"
     gene            complement(5268..5561)
                     /locus_tag="PK742_03305"
     CDS             complement(5268..5561)
                     /locus_tag="PK742_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75644.1"
                     /translation="MNFFQRDSVLVGIVVSVFFSITMFYCLHTINSMLIGRPFGGASF
                     QGVTERFVSTLAVFFNIIPFVIYLRTRKDLSMKGVGIVTVLLALFVLVFYYIL"
     gene            complement(5563..6384)
                     /gene="surE"
                     /locus_tag="PK742_03310"
     CDS             complement(5563..6384)
                     /gene="surE"
                     /locus_tag="PK742_03310"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQU75645.1"
                     /translation="MAEKPTILVTNDDGFHAPGIHALVAAMKEIGQVIVLAPDKPQSG
                     MGHAITINDPIRLQQVDVFGDVEAYACSGTPVDCVKLAKDKILHHKPDLCVSGINHGS
                     NSSINIIYSGTMSAAMEAAIEGIPAIGFSLLDYAHDADFRPSAHYASQIARQVLENGL
                     PDNTLLNVNIPALPVQEIRGMRICRQAVAKWEEEYDERNDPRGHRYFWLTGKFVNYDQ
                     GDDTDEWALDNGYVSVVPVQFDLTAHHAISFFQKNWDLHQTHPESHSGRSVKDLG"
     gene            complement(6387..8243)
                     /locus_tag="PK742_03315"
     CDS             complement(6387..8243)
                     /locus_tag="PK742_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75646.1"
                     /translation="MRRSFSIGLLLLLPLITWSQSEKRFIKSANKLFEAGAYAESAAI
                     YLILLQQHYQDDLNRNLAVCLEQMGDHKAAEYWYEKYLTVHPEDMPALLSYAHLLKYN
                     GKYHLAKEAYLLYATADPVGYYFAGTCDYAINNQDRTNTCFIEGLGINTPGSDISPAF
                     INRGIMFSSNGDLGVEGKKQENDPGSGSPFYSLYYVSEENTNYQASVQPLRDINQQYN
                     NAAVSYDQMLDRIFITRNNYLHGRLIKDKDRMVQLSIMEGLIDGLSVYKLRPFEWNDK
                     TYSVGQPFVLPGGDLLLFVSDMPGGFGGTDIYYCINTGDGWSEPINMGAPVNTPGNEM
                     YPFLSDQQILYFSSDYLPGFGGLDIFMSARDGQHWSKPVNMGLPLNSSQDDFGLILTK
                     GSGYFCSNRPGGAGNDDIYRVAFLKPVADLNVINARLQPVADALVVLYENDHRVMEAE
                     TDLSGHVTLPVEEGHQYSVHISKDGYMESVVDFLDQYRSTTGSMEVSLLEYYDYIDES
                     EWEYDIPGNSGGEQDTADNEQGRDEHDSEQHDGMLTYELFIGSFEDPDYTRITSLARF
                     GELSVRQTGRNVLEFYIINLDHETALKAHEAAIAAGFQTSRVITYRNKERID"
     gene            complement(8245..9195)
                     /locus_tag="PK742_03320"
     CDS             complement(8245..9195)
                     /locus_tag="PK742_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQU75647.1"
                     /translation="MNRRCIYLILLLLPLLGQTQNEPVFDQYRFNQLVLNPAYAGSQG
                     TLEGTAMARRQWVGLEDAPGTESVILHAQLANGKIGLGGKLLHDQIGPTEKTTYALDY
                     AYRMYMGSGTLAVGLELSAANYRLNYTQLDAYQDGDPAFTGVPGQLMSYNAGLGVWFN
                     SKRFYSGLSVPNVVSKADENDPLAPVLNELDPYIQTIHAYWTGGYMLQLSRNVALLPH
                     GMVRYDLHAPIVYEGGLELLFREQVQLGAAWQSVEALTIKGEFFLDLNNTLSDRALGV
                     GYAYNQRLDETQPVFGSTHEVFITLQWNKRATRFVSPRFF"
     gene            complement(9192..10376)
                     /locus_tag="PK742_03325"
     CDS             complement(9192..10376)
                     /locus_tag="PK742_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQU75648.1"
                     /translation="MIRHHLFLLLLIAAVSGVHAQMIAVDDVVTVNEDEEVVIDVQFN
                     DINLGGGLTTTFLYTTPPNGSLELLDNDSIRYTPDPDYNGVDTFSYQVCNGVEPVPEC
                     DFALVIVTVLPLPDYPIASDDTVITTVATSVVADVQANDINLDAENLVTAVVTPPSNG
                     TAVVILTDSILYTPAGFYTGWDSLQYSVCKTGSSIYCDSAWLFIRVDTVNFFPPVALD
                     DSFMVDAGSETMLDVLVNDFDGDGDALVLSEIIPVSGLGFASIESNMIKYLAIGTGTD
                     TFFYSVCDANLPSYCDTAMVVVTMLDIDIPDSFSPNNDGFNEIFEINGLVQYPGFELS
                     IFNRWGQLVYRTTEPDFKWHGEISIDGANSSDEVVQGTYYYFLQLNNGFEPLTGSIVL
                     RR"
     gene            complement(10379..12004)
                     /gene="pruA"
                     /locus_tag="PK742_03330"
     CDS             complement(10379..12004)
                     /gene="pruA"
                     /locus_tag="PK742_03330"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQU75649.1"
                     /translation="MSNAFFRVPAAINEPVKGYAPGSPEREKLKQALSELKAATADIP
                     MFINGQEVRTGKTVTMHPPHELSHTLGHYHKGGKEHIQQAIDAALAAKHDWENTPWEQ
                     RASIFLKAADLLAGPYRYKMNAATMLAQSKNPFQAEIDAACELIDFWRFNVQYMAQLY
                     AEQPESQPGMWNRMEHRPLEGFVFALTPFNFTSIAGNLPTAPALMGNTVVWKPANSEI
                     YSAWVIMEILREAGLPDGVINLVYVGGVEAGEVIFSHPDFAGIHFTGSTQTFQTIWKT
                     IGNNIAGYKSYPRIVGETGGKDFVVAHKSADAMEVATALVRGAFEFQGQKCSAASRAY
                     IPDNIWPAVKEKMLTDLASIKMGGVEDFGNFFNAVIDEKAFDSIAGYIDQAKADGLEI
                     IAGGGHDKTKGYFIEPTVILAKDPAYRTMCEEIFGPVLTIFVYPAEEWTATLKLVDST
                     SPYALTGAIFSRDRYAINEGVNLLRHAAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     AGSILNLLRWVSPRAIKETFVPPTDYRYPFLHS"
     gene            12003..12911
                     /locus_tag="PK742_03335"
     CDS             12003..12911
                     /locus_tag="PK742_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQU75650.1"
                     /translation="MYRGICCKDSQFDAGESILTAIVTGILGDVSQNNYFHFPHMKKK
                     ARKSTDSALWVKENLQQWKKDVRAYKRCGKAEDLHKLRLDCKRLQAYVYFLKLVAPDR
                     VKRKEWKAWQNLYELSGKIRDHQQHHDLLIRMQFPEVADALFHPRLTDSMHKQWKKAY
                     RKSSPSINKGYRKLIRIAGNRKITKGMNKAIVHGWKELEDMLTHDNSQFELHDLRKMI
                     KRALFARQFLTDNKYKQPKKLITYCKRLEEILGKWHDQEKLEKWMTANGSGLSGGTAQ
                     HICDLSRAQRHTYIVDLERILAEFRL"
     gene            12923..13150
                     /locus_tag="PK742_03340"
     CDS             12923..13150
                     /locus_tag="PK742_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75651.1"
                     /translation="MRSKNLLSLFCAALLMLSAAGCTICSTCTYSYYYYGVESTYEEQ
                     YCGSRYEVRNFEDDFIDGAQQQNVSTDCNRH"
     gene            13241..>15488
                     /locus_tag="PK742_03345"
     CDS             13241..>15488
                     /locus_tag="PK742_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQU75652.1"
                     /translation="MATKKKKKQPARSVNLRGFIRDERTTRTAGILLILLSFFLLVSF
                     ISYLFTWSKDFSLVQENTVGGVFRTDRKASNQLGNLGAATGHLFFYKWYGIASFLFVW
                     WLLILGVNTFFSRRVFPLRKHFFRGILWLVWLSTALGFILYNQPFPWGGAFGKGMTDS
                     LEAVTGKIGTAAILVVTALIYLVVFHRIDLQWKKSPKEELPADEHDKPVSDAPVYTSS
                     GDIISAEDGQENETEIAEDEDDPAPEMELHTITRDPAEIIKPAVDPLPSDDDNMELEI
                     AEAVEEKIIDDPSLGHYDPIMDLPDYKYPAIELLEDHQSEGVAIDTNELERNKDQIIS
                     TLSNYNIQISKIKATIGPTVTLYEIVPAAGVRISKIKNLEDDIALSLAALGIRIIAPI
                     PGKGTIGIEVPNVNKEVVSMRSQIASEKFQQAKMDLPICLGKTISSENYIADLTRMPH
                     LLMAGATGQGKSVGINSILISLLYKKHPSQLKFVMVDPKKVELSLFSAIEKHFLAKLP
                     GEEEAIITDTRKVVNTLNALCIEMDTRYELLKNAQVRNIKEYNAKFMRKKLNPKEGHM
                     YLPYIVLVIDEFADLIMTAGKEVEMPIARIAQLARAVGLHCIIATQRPSVNIITGTIK
                     ANFPARVAFKVASKVDSRTILDASGADQLIGRGDMLLSIGSDIVRLQCPFVDTPEVDE
                     VVQHIAHQPSYPEAFLLPEYVDEKDVKERGEMDDDRDELFDEAARIVVQHQQGSTSLL
                     QRRLKLGYN"
BASE COUNT         4016 a         4001 c         3678 g         3793 t
ORIGIN      
        1 aaaaaaacag gaacactgta aggtattcct gtttccgtgg agacagtggg acttgaaccc
       61 acgacctctt gcatgccatg caaacgctct agccaactga gctatgcccc cgaagagggt
      121 gcaaatatac agggaattgg caaatcggaa aagcctcaat aagctaccac aaatggcagc
      181 acctgcctgc tgccatctgt aaagatcagc tccaggtgat aggttccagg tgcagagatc
      241 aggacatgtt gctcctggtc tgaaagtcca acattcaccc ggaactgtct cacgaccctg
      301 cctgcaacat ctgtgatcct gacctgatag ccctctcttg cgttcatggt cgttctccag
      361 tggatccagc catcggccga aggattggga tacagggtta ttcctccggc aacttccgtc
      421 tccagagcag tgctgcctgc aaacgccttt gcatcggccg agatagaaaa ctgtcccagc
      481 cagctgtcgc ccgcaggaga aaaggcaaga gtgcttcccg cggcctcatc tgcggtagca
      541 aacagatctc cccaggaatc tccttcggaa tagtatggcc tgttgtataa ctccacatat
      601 tcaggatggg cagtcatact gtcgttgata ccacctatgg acgtaaaact gatcgcattc
      661 agagatgcgg tgccagcgcc gttgaactgc ctcacaataa aataccgctc cggatcgggt
      721 aacaaagaat cgccgggaac cacatccgga cgggcatgca aacgggatac caccacatct
      781 gttccgggca cttctgccca tgtgagtgta actcctgtgt gatcaaatac taccggatct
      841 tccgtatctg catacaccct gtcaaacgta ccgccggcca ctggagctgt agatgcgatc
      901 tgtactgcac tgccttccgg taaggcctga ttaccaccag ctttattata gaaatagccg
      961 acactttcgt tgaactgata gtaggctatc aaagattcat catcctgcgg caggttgtcc
     1021 ggatgcggat tgttccgcgt caggtgcatg agatcctgta tctcttcttc actcatggaa
     1081 cgatcatata cggccacttc atccatctcg ccttttatat atcgcccgcc ccagtgctta
     1141 taggaaccca gtaatacatt attattttct aaatttgttg gcgagaccgt gatgttgtgt
     1201 ttgcttcctt caccgttcac atacagtgtg atgccgtcag gttccaccac catggccagg
     1261 tgcgaccagc ggccttcggg tacaatcagt ccactctccc accaccaggc accgcccggc
     1321 cagtggtacc ccagtttgtt atcgccggga cggaaattga atccggctgc atcagatcct
     1381 tccagcatga acaaagagct gtagtctgtc tggatgccat cgggtttgaa ccacatggaa
     1441 atggtcaggt ggttggtggt aatacccaaa ggcggaacgg acacatctgc aggatcggca
     1501 ttaccgttca gggcgatagc cataccggca acagtatcga ctccacattc atttccggaa
     1561 atgctgatgg ctcctgtttc tgttttcgtg tctgttcccc aggcattgga aatggtcagg
     1621 gtgaagtcat acaccccttc tgcgccaaac accactttcg gatttctggc agttgcattg
     1681 cttacaaatt caggagctgg tgaaaaactc caggaccagg tggcaccact gtgatccatg
     1741 accgagtaat cttcgaaata aaaagtatcc agcgcacagg cagataccag cttgtcaact
     1801 gtgggctgcg caatgggtgc ataggtatct tccaggtcac gttcccatac ccccctgttt
     1861 cctgctaccc ggatcttgct gtcgcggtag aaggcaaagc ttctgatggg tctgttacca
     1921 gccggcagtc cgtctgatac atctgtccag tctgccatgg tatcatccct atagaaggcc
     1981 gctccgttat tacccagcac atacacacca ccttcagaac ccttgtggtg caccacacag
     2041 gcatagcctt gtccgtctat cagagtggta gtgagattct cccagctgac accaccatcg
     2101 tgcgtcacat atactctttt accagcagga ttggaaggag aggatatcca taccgtttgc
     2161 ggatcctgcg catcgatggc cagatccatt ctataagtgg atacacctgt gggtagtgta
     2221 agattagtga atgaggtgcc gccatcatca gatcggttga gtttgttggc agtggccaca
     2281 tatatggttt gcggatcact tctgctgatc tcaaatttct tgaccttatt acctataccg
     2341 gcaccaaaat catacagggt agtgaagctc gccccgaggt tatcagaacg ccatagcgca
     2401 ttcccgtttc ccaggaaaac caccccatag cagtaaggaa gaaactctac atcgctggca
     2461 tcaaaaccgt atccatcctc attggggtac atgccaaatg caaaatattt tctgggaccg
     2521 tcaaaagaaa gaggaacctg aaagtttcct atatcagaaa aggcgaaata accttctctt
     2581 cccgccatgg cataaccggt gcccgcttct ccgccaccca gacgaatgga agctccttcg
     2641 ggatattgat ctgataagga tgtgttgcca ttgtggtatc gtccgcctgc catatagtct
     2701 gcattccatc cctggctgaa gccccagaaa tctgtgccga aaatgccatt ggtgcgggat
     2761 tcataattag ctgtggaagc aaaaaagtct gtagagtagt tcactccgcc atcagtggtg
     2821 atccaggtat ctccatctat ggcgatcatt tcctggatat ccggatggat gttgaaggaa
     2881 cctccgtatc cacccaggcg caggaaactg ctgccaccat tggtagagcg ataggcagta
     2941 gtagaagcca cgatgacctc atctgcattc aggggatttg ccacgatatc cagatcataa
     3001 aaaccctgac cattgctcat tccgagtggt gaggagtcat caccggtaag gccactctca
     3061 ataccgatgc acatgagttc ccagcttata ccaccatcat ctgaacgata cacatgcgga
     3121 gcggttacgg aaggaagcgt gccgagggca accacataca cccgctcagg atcatcggca
     3181 gtcagagaca tacgggcacc agaggtattg accggagtaa caccggcaaa cccgctttca
     3241 aaggtgacac ctgcatcgtt gctgcgcatg cagtctatca caccagactg aacccggatg
     3301 gcgatgaccc tttccggatc tgttgtatga aaatacacat cgtacatatg tgatgtgatt
     3361 ccatctgtta ctgcaaatga aagtccgccg tcagttgatc tgaataaacc atagtctgtt
     3421 gccagcaaaa ctttctgcgg atcagaagga tgaaaagcaa tcgagtgaat atttccggca
     3481 gcgagagaca tagtggtcca gctgctgccg ccatcggtgc ttcttgcaag gcttccgtta
     3541 gctccggcca gcatcagatc cgggtcgccg ggagaaatgg ccactgatcc ataggttgtt
     3601 gcattatcca gtgccagtgt ccatgccaga cctttattcg aagaatagaa caggccgcct
     3661 gtttctgcag cagcaagcat tctgtcgggg ttgttgacat cggcagtgat agcatagaca
     3721 ttcacctgcc acggacattc cggttgagac ggatcatcgg ccgcatcaaa atgtgtggtg
     3781 gccggtcctg ccagggtcca ggaacctgtg gccttagcat gcactgttcc ggtggctgat
     3841 acttccactt gtgccacact gccatcggcc tgaatgaacg gttccaccga ccttctccag
     3901 cgctggtagt agaattccca gatcgttctg tgttcttctt ccggcatccc gttcagatga
     3961 cttgcatcaa acagactgtc catgacaaag acatttggaa tctcatcagc atacaacggc
     4021 aggaaccagt caggggtttg cgcatcgggt tcaggcagat atactccacc ggtcttttgt
     4081 gctgaaacag aaacagacag aagcaggcaa aaacctgcaa ataacttgta cataaaacaa
     4141 atttgtagtt taaagttaca aagccttgtg caaggcatca gtaataatgg cggctgctct
     4201 ggaagaggcc ccttgtccgc caagcttcaa gcgcaattcc atatagccct gttgcatgat
     4261 cgctgccttt tcaggttgga gcaaatcatc gagatcccgc agcaattgcc ttctgttaaa
     4321 actgctctga atacgttcag gtaccagttc actgttcatg atgaggttga ccaggctgat
     4381 gaaacgaacc ttcaccagtc ttctggcgat ccagtatgac agggtgctac cacgataaca
     4441 taccacctgt ggaacaccaa acagggcggt ctccagggta gcggttccac tggttacaag
     4501 ggcggccttc acatgctgca gaagtgcata agtctgacca tgcaccaggt gcacattggc
     4561 aggcagatcg aaagatgcat atgctgccgc atccagtgag ggagcacccg cgatcacaaa
     4621 ttgtaaatca gctctttcat tggcaacctc cagcaagacc ggcatggatc gtttcagttc
     4681 ctgtttgcgg cttccgggta acaaggctac cagggagcca tcgggcaagg agtttttagg
     4741 aagaaaatcc ggttctgcat ccatatcgct gatctcgtcg agcagtggat gtcccacata
     4801 ttccacctga taatcatagc cttcataaaa ggctttttca aatggcagaa tgaccagcat
     4861 gtggtcaaca tactgtttga tggtctttac ccgtccggct ttccatgccc acaactgagg
     4921 tgagatataa tagaccgttc tgatgccaag tgatttgatg aatggcagca tgcgcaggtt
     4981 gaatccgggg taatcaatgc agatgaccac atccggctga aagttactga tgctttgttt
     5041 tgctgttttg aaattggcag agatcttgcg caggtgcagc agcacttctg taaaacccat
     5101 gaaggccagc tggtcaatat gccggtcaag ttgtacgcct tcggcttgca tacgctttcc
     5161 gccaaaacca cgaaactgtg cctgggtatc ctgttcccgg agggccttca ccaggttggc
     5221 tgcatgcaga tcgccgctgg cctctccagc caggatatag tacttcatca gagtatatag
     5281 tagaatacca gcacaaacaa ggccagcaaa acggtcacga tacccacccc tttcatactc
     5341 aggtctttcc tggttcgcag ataaattaca aagggaatta tattaaagaa aaccgcaagt
     5401 gtagaaacga aacgttcggt aacaccctga aaagaagcac cgccaaatgg ccggcctatc
     5461 aacatggaat tgatggtgtg caggcaataa aacatggtga tggaaaagaa tacagacacc
     5521 actataccta ccagaacgga atcacgttga aagaaattca tatcagccca ggtcttttac
     5581 ggatctgccg gaatggcttt ccggatgtgt ttgatgcaga tcccagttct tttgaaaaaa
     5641 tgaaatggcg tgatgagcgg tcaggtcgaa ctgcaccggc accacagaaa cataaccatt
     5701 gtccagggcc cactcatctg tatcatcacc ctgatcatag ttcacaaatt ttcctgtaag
     5761 ccagaagtag cgatgtcctc tcgggtcatt tcgttcatcg tactcttcct cccatttggc
     5821 taccgcctgc ctgcatatgc gcatacccct gatctcctga acaggcaggg cgggtatatt
     5881 gacattcagc agggtgttat caggcaaacc gttttccagt acctgccggg ctatttggga
     5941 tgcatagtgt gccgatggtc tgaaatcagc atcgtgtgca taatcaagca atgaaaaccc
     6001 aatagccgga ataccttcta tggcagcttc catagctgca gacatggtac ctgaataaat
     6061 gatattgata gaagagttgg aaccatggtt gatacctgac acacaaagat ccggtttgtg
     6121 atgtaggatc ttgtcttttg ccagtttcac acaatctacc ggtgtgccgg agcaggcata
     6181 ggcttctaca tcgccaaaca cgtccacctg ctgcaatctg ataggatcat tgatggtgat
     6241 ggcatgaccc attcccgatt gcggtttgtc aggtgccaga acgatgacct gtccgatctc
     6301 tttcatggct gccaccagag cgtggatgcc cggagcatga aaaccgtcgt cgttggtgac
     6361 cagaatggta ggtttctctg ccatactcag tctattcgtt ctttgtttcg gtaggtgata
     6421 actctggagg tctgaaagcc tgcagcaata gctgcttcat gtgcttttaa tgctgtttca
     6481 tgatccagat tgatgatata gaactccagc acattacggc ctgtttgcct gacactaagc
     6541 tctccgaatc tggccagtga agtaatgcgg gtataatcag gatcttcaaa ggagccaatg
     6601 aacaattcat acgtaagcat gccgtcgtgt tgttctgagt catgttcatc ccggccctgt
     6661 tcattgtcag cagtgtcttg ctcaccaccg gagttacccg gaatatcata ctcccattct
     6721 gactcatcta tataatcata atactccagc aaactcactt ccatactgcc ggtggtgctt
     6781 ctgtactgat ccagaaaatc aactacagat tccatatagc catccttgct gatatgcaca
     6841 ctgtactgat gcccttcttc aacaggcaga gtcacatgcc cgctgaggtc ggtttctgct
     6901 tccattaccc gatgatcatt ttcatagagc acgaccaggg catctgccac cggttgaagt
     6961 ctcgcattga tcacattcag atcagcgacc ggcttcagaa aagccacccg gtagatatca
     7021 tcatttcctg cccctcctgg tctgttgctg cagaaataac cagaaccttt ggtcaggatc
     7081 aatccgaagt catcctggct gctgttaagc ggcaagccca tgttaactgg tttgctccag
     7141 tgctggccat ctctggcaga catgaaaata tccagaccac caaaaccagg cagatagtct
     7201 gatgaaaagt acagtatctg ctgatcagac aggaagggat acatctcatt tccgggagta
     7261 ttgacaggag cacccatatt gatgggttct gaccaaccat cgccggtgtt gatgcaataa
     7321 tagatgtctg tgccgccaaa acctccgggc atatcgctca cgaacagcag caggtcacct
     7381 cccggcaaga cgaaaggctg ccctacagag taggtcttgt cgttccattc aaaaggtcga
     7441 agtttataaa cactcaaacc atctatcagg ccttccatga tgctgagctg caccatccgg
     7501 tctttgtctt tgatcagcct gccatgcaga taattattcc ttgtgatgaa aatacgatcc
     7561 agcatctggt cataggatac tgctgcatta ttgtattgct ggttgatatc acgcaatggc
     7621 tgaacagatg cctggtaatt agtattttct tcagatacgt agtatagtga atagaatggt
     7681 gatccagaac ccggatcatt ttcctgtttt ttgccttcca cacccagatc gccattggat
     7741 gagaacatga ttccgcgatt gatgaatgcg ggcgatatgt cagaaccggg agtattgatg
     7801 cccaatcctt ctatgaagca ggtattcgtt ctgtcctggt tattgatggc ataatcacag
     7861 gtgcccgcaa agtagtatcc caccgggtca gcagttgcat agagcagata agcctcttta
     7921 gccagatggt acttgccgtt gtacttcagc aggtgagcat aggaaagtaa ggcaggcata
     7981 tcttccgggt gaaccgtcag gtatttctcg taccagtact ctgcggcttt atgatcgccc
     8041 atttgttcca gacaaacagc caggttgcgg ttgagatcat cctggtaatg ttgttgcaac
     8101 aggatcagat aaatggcggc actttctgcg taggcgccgg cttcaaacag cttgttggca
     8161 gatttgatga aacgcttctc agactgactc catgtaatca atggaagcag cagtagcaga
     8221 cctatggaaa acgatctacg catatcagaa gaaacgcggg ctgacaaaac gtgttgcccg
     8281 cttattccat tgtaatgtta taaaaacctc gtgtgttgac ccaaataccg gctgggtctc
     8341 atccagtcgc tgattatagg catacccaac acccagagct ctgtctgaaa gtgtattgtt
     8401 caggtccaga aagaactctc cttttatggt cagcgcctct acagattgcc aggcagcacc
     8461 taattgcacc tgctctctga acaggagctc caggccacct tcatacacga ttggtgcgtg
     8521 cagatcatac ctgaccatac catgcggcaa cagggcaaca ttcctggaaa gctgcaacat
     8581 ataaccaccg gtccagtaag catgaatagt ttgaatgtat ggatccaatt cgttgagtac
     8641 cggggcaagc gggtcattct cgtctgcctt gctgaccaca ttaggaacag ataatccgct
     8701 gtaaaaccgt ttgctgttga accacacacc cagacctgca ttgtaagaca tcagttgccc
     8761 gggtactccg gtaaacgcag gatctccatc ctgatacgca tcaagctggg tataattcaa
     8821 ccggtaattt gctgcagata attccaggcc tacagccagg gtacctgacc ccatatacat
     8881 ccggtaggca tagtccagtg cgtaggtggt tttttcggta gggccaatct ggtcatgcaa
     8941 tagctttccg cccagcccga tcttaccatt ggcaagctgc gcatgcagaa tgaccgactc
     9001 tgttccgggt gcatcttcca gtcctaccca ttgccttctg gccatggctg ttccctccag
     9061 agtaccctga cttccggcat aagcgggatt gagcaccagt tgattgaacc tgtactggtc
     9121 aaataccggc tcgttctgtg tctgccccag aagaggcagc agcagcagga tcagatatat
     9181 gcacctcctg ttcatcttct cagtactatg cttccggtca acggttcaaa accattattt
     9241 aattgcaaaa aataataata ggtaccctgt accacttcat cactggagtt ggctccgtct
     9301 atgctgatct ctccatgcca tttaaagtct ggttctgtgg ttcggtaaac aagctgcccc
     9361 cagcgattga aaatgcttaa ttcgaatccg ggatattgca ccaggccgtt gatctcaaag
     9421 atctcattaa atccatcatt attcggactg aaagaatccg gaatatcaat atccagcatg
     9481 gtaacaacta ccatggctgt atcacaatag ctgggcaggt tggcatcgca aactgaatag
     9541 aaaaatgtat ctgttcctgt tccgatggcc agatacttga tcatatttga ttctatactc
     9601 gcaaaaccaa gaccagaaac cggaatgatc tcactgagaa caagggcatc cccatcgcca
     9661 tcaaaatcat tgacaaggac atccagcatg gtttcggaac ctgcatccac catgaaggaa
     9721 tcatccagtg ctacaggcgg aaaaaaattg accgtatcta cacggatgaa caaccaggca
     9781 ctgtcacaat agatggaaga gcccgtcttg cacacactgt attgcagaga gtcccatccg
     9841 gtatagaaac cagccggtgt gtacaggatg ctgtcggtca gaatgaccac cgcagttccg
     9901 ttggaaggag gggtcaccac ggcagtaacc agattttctg catccagatt aatatcattt
     9961 gcctgtacat cagctactac agaagtggcc acggtggtaa tgacagtatc atcggatgcg
    10021 atcggataat caggcaacgg aagcaccgtc acaatgacca gggcaaaatc acattcgggt
    10081 acgggttcta ctccattaca gacctggtag ctgaaagtat ccaccccatt atagtcagga
    10141 tccggagtgt accggatgct gtcattatcc agcaactcaa ggctgccatt gggtggcgta
    10201 gtgtacagga aagtagtggt aagcccacca cccagattga tatcgttgaa ctgcacatca
    10261 atgaccacct cttcatcttc attcacggta actacatcat caaccgctat catctgggca
    10321 tgtacgcccg agacagcagc aatgagcagc aggagaaaca ggtggtggcg tatcatattc
    10381 agctatgcag gaatgggtat ctgtaatcag taggtggtac gaacgtttcc ttgatggccc
    10441 gcggagaaac ccaacgaagc aggttcagaa tggaaccagc cttatcgttg gtaccggaag
    10501 ccctggcacc accaaatggt tgttgtccta ctacagcacc agtcggttta tcgttgatat
    10561 agaaatttcc ggcggcatga cgcagcaggt tcactccctc attgatggca tagcgatcgc
    10621 gggagaagat ggcaccggtc agtgcgtaag gtgaagtact gtcaacaagc ttcagggtgg
    10681 cggtccactc ttctgccgga tacacaaaga tggtcagcac gggtccgaat atctcttcac
    10741 acatggtacg gtatgcagga tccttcgcca gaataacggt gggttcaatg aaatatcctt
    10801 tggtcttgtc atgtcctcct ccggctatga tctccagtcc gtcggctttg gcctggtcaa
    10861 tatatccggc tatgctgtca aaagctttct catcaatgac ggcattgaag aagttgccaa
    10921 aatcttccac acctcccatt ttgatgcttg ccagatcggt cagcatcttt tctttgactg
    10981 ccggccatat attgtcagga atgtatgcac gggacgcagc agaacatttc tgaccctgga
    11041 attcaaatgc tccccttacg agtgcggtgg ctacttccat cgcatctgcg cttttgtggg
    11101 ctactacaaa gtcctttcct ccggtctctc ccacgatacg cggataactt ttgtatccgg
    11161 ctatgttatt tccaatcgtc ttccaaatgg tctggaaggt ctgggtggat cctgtaaagt
    11221 gaataccggc aaaatccggg tgactgaaga tcacttcgcc ggcttctact ccgcccacat
    11281 acaccaggtt gatcacccca tcaggcaatc ctgcttcgcg aaggatctcc ataatgaccc
    11341 aggcagaata gatctctgag ttggcaggtt tccaaaccac agtattgccc atcagagccg
    11401 gtgctgtagg cagattaccc gctattgaag taaagttgaa cggtgtcaga gcaaacacaa
    11461 aaccttccag aggccggtgt tccatcctgt tccacatgcc cggctgactt tcaggttgct
    11521 cggcatagag ctgtgccatg tactgcacat tgaaacgcca gaaatctatg agttcgcagg
    11581 cagcatcgat ctctgcctga aaaggattct tgctttgtgc cagcatggtg gccgcattca
    11641 tcttatatct gtatgggccg gccagcagat cagctgcttt caggaaaatg gatgcccgtt
    11701 gttcccatgg ggtattctcc cagtcgtgct ttgcggcaag tgcagcatct atggcctgct
    11761 gaatatgttc cttacctccc ttgtggtagt gaccaagggt atgactcagt tcatgcggtg
    11821 ggtgcatggt caccgttttc ccggtgcgta cctcctggcc gttgatgaac atcggaatat
    11881 ctgctgttgc tgcctttagc tcgctcagag cctgtttcag cttttctctt tcggggctgc
    11941 ccggagcata tcctttcacc ggctcattaa tggctgcagg taccctgaaa aatgcattgc
    12001 tcatgtatcg tggaatttgc tgcaaagata gtcaattcga tgcgggcgag tccatcctga
    12061 cagccattgt gaccggaata ttgggtgatg tcagccagaa caattacttt cattttccgc
    12121 atatgaaaaa aaaggcacgc aaatcaactg attctgcgct gtgggtgaaa gaaaacctac
    12181 aacagtggaa gaaggatgtg cgtgcatata aaaggtgcgg caaagcggaa gatctccaca
    12241 aactcaggct ggactgcaaa cggctccagg catacgtgta cttcctgaaa ctggtagcac
    12301 ctgatcgtgt caaaagaaag gaatggaagg cctggcaaaa tctctatgag ctgtctggta
    12361 agattcgcga tcaccagcaa caccatgatc tgctgatccg aatgcaattt cctgaggttg
    12421 ctgacgccct gtttcatccc cgactgaccg attccatgca taaacaatgg aaaaaggcat
    12481 acaggaaatc ttctccttct atcaataagg ggtaccggaa attgatccgc attgcaggca
    12541 acaggaaaat cacgaaaggc atgaataagg ccatcgtaca tggctggaag gaactggaag
    12601 acatgctgac ccatgacaac tctcagtttg aactgcacga ccttcgcaaa atgatcaaac
    12661 gggcattatt tgcccgtcag tttctgacag ataacaagta caaacagccc aaaaagctca
    12721 ttacctactg caaacgactg gaagaaatat tggggaaatg gcatgaccag gaaaagctgg
    12781 agaaatggat gaccgccaat ggctctggac tctccggagg aaccgcccag catatctgtg
    12841 acctaagccg tgcacagcga cacacctata ttgttgatct ggagcgcata ttagctgaat
    12901 tcagattgta actttaccat acatgcgatc aaagaatctc ctgtcactgt tttgtgccgc
    12961 cctgctgatg ctcagtgccg ccggctgtac catatgctct acctgcacct actcctacta
    13021 ttattatgga gtggaatcta cctacgaaga gcaatactgc ggaagcaggt acgaagtgcg
    13081 caacttcgaa gatgatttca tagatggtgc acagcagcaa aacgtcagta cagattgcaa
    13141 ccggcactga tcttccacaa tcctgtacac cctagagcgt ttctgcacaa tagccgcctg
    13201 ttaacacaaa tggcatattg tatttttacc tccacctcac atggccacca aaaagaagaa
    13261 gaagcaacca gcacgttcag taaacctccg tggttttatt cgtgatgagc gtaccaccag
    13321 aactgccggc atcctgctga tcctgctaag cttttttttg ctggtatctt tcatcagcta
    13381 cctgttcacc tggagcaaag atttcagcct ggtacaggaa aatacggtag gtggtgtgtt
    13441 ccgcaccgac agaaaagcct ctaatcagct tggaaatctg ggtgcagcta ccggccacct
    13501 gttcttctat aaatggtatg gcatagccag cttcctgttc gtctggtggc tgctcatcct
    13561 gggtgtcaat accttcttca gcagacgggt cttccccctt cgtaagcatt ttttcagagg
    13621 aattctctgg ttggtctggt taagtaccgc actgggtttt atcctctaca accaaccttt
    13681 cccctgggga ggcgccttcg gaaaaggaat gacagacagc ctggaagcag ttaccggcaa
    13741 gataggaacg gctgccattc ttgtagtgac agctcttatt tatctggtgg tctttcatag
    13801 aattgatctg cagtggaaaa aaagtccgaa agaagagttg ccggcagatg aacatgacaa
    13861 accggtatct gatgcccctg tgtacacctc ttcaggtgat atcatttctg ccgaagatgg
    13921 tcaggaaaac gagacagaga ttgcggagga tgaagacgac cctgctcctg agatggagct
    13981 gcacaccatt acccgagatc cggcagagat catcaagcct gcagtggatc ctcttccctc
    14041 cgacgacgat aacatggaac tggagatagc tgaggccgtg gaagaaaaga tcattgatga
    14101 cccttctctc ggccactacg atcctatcat ggacctgcct gattataaat atccggccat
    14161 cgaactgctg gaagatcatc agtcagaagg agttgctatt gataccaatg aactggaacg
    14221 aaacaaagac cagatcatct ccaccctcag caattacaat atccagatct ccaagatcaa
    14281 ggcaaccatc ggccccaccg taaccctgta tgagatcgtt ccggctgccg gtgtgcgcat
    14341 ctccaagatc aagaacctgg aagacgatat cgccctgagt ctggcagctc tgggcatccg
    14401 catcatcgcc ccgataccgg gcaagggaac catcggtatc gaagtgccta acgtgaataa
    14461 ggaggtggtg agcatgcgtt cacagatcgc gtcagagaaa ttccagcagg ccaagatgga
    14521 cctgcccatc tgcctgggta agaccatctc cagcgagaac tatatcgccg acctgacccg
    14581 gatgccccac ctgctcatgg cgggtgctac cggacagggt aaatcggtgg gtatcaacag
    14641 catcctcatc tccctgttgt ataaaaagca tccatcgcaa ttgaagttcg tgatggtgga
    14701 tcccaaaaag gtggaactgt cattgttctc agctattgaa aaacatttcc ttgccaagct
    14761 gcctggtgag gaagaggcca tcatcacaga tacccgcaag gtggtcaata ccctgaacgc
    14821 cctatgcatt gagatggata cgaggtatga actcctgaag aatgcccagg tgcggaacat
    14881 caaggagtac aatgccaagt tcatgcggaa gaagctcaac ccgaaggaag ggcatatgta
    14941 cctgccttat atcgtgctgg tgatcgatga atttgccgac ctgatcatga cggcaggtaa
    15001 ggaagtggag atgcccattg ccaggatagc ccagctggca agggctgtgg gactgcattg
    15061 tatcatcgcc acccagcgac ccagcgtcaa catcataacc ggtaccatca aagccaactt
    15121 ccccgccagg gttgctttta aagttgccag caaggttgac agccggacca tcctggatgc
    15181 cagtggtgcc gatcagctga taggccgcgg cgacatgctc ctgtccatcg gcagcgatat
    15241 tgtgcgcctg caatgtcctt ttgtagatac tcctgaagtc gatgaggtgg tgcagcatat
    15301 tgcccaccag ccctcctacc cggaggcctt cctcctgcct gaatatgtgg atgaaaagga
    15361 cgtaaaggaa cgcggtgaaa tggacgatga ccgggatgaa ctgttcgatg aagccgcccg
    15421 tatcgtggta cagcatcagc agggttccac ctccctgtta cagcgaaggc tcaaactggg
    15481 atacaacc
//
LOCUS       DAOMAX010000035        15406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_433_length_15406_cov_4.668947, whole genome shotgun sequence.
ACCESSION   DAOMAX010000035 DAOMAX010000000
VERSION     DAOMAX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_433_length_15406_cov_4.668947"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PK742_03350"
     CDS             <1..646
                     /locus_tag="PK742_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75653.1"
                     /translation="SIRMVLLMIGSLLVVLLFALPVLGMQASWLQQQLSDPFAIANLK
                     ADAGWNWFDLLPGLVLLTGMTAGFYAMRNQLLYGLFVLFVSVLLTGKTGMVMLAPKVE
                     AYTQRAAVEFFSQHRDEDAYLAAIGYKTYADAFYGRMQPAGKPRLEDYSDKKQKADWD
                     NWLLAGNIDRPVYFITRMGKESYLESKPDIQKLYEKNGFLMYYRPVPPPGGNLQ"
     gene            753..1922
                     /locus_tag="PK742_03355"
     CDS             753..1922
                     /locus_tag="PK742_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HQU75654.1"
                     /translation="MKQLIIGLLLSAPLMLAAQDVHFFQGTWEEVNAKAKAENKPIMV
                     DAYTVWCGPCKMMDKTMFHDNAAVADLLNSSVIPYKIECEHDFGLTFNQKFRVAGYPS
                     LLFFNSDGQLFDRLLGYDDNTANFVSTLKEVLSGDQQDTYGYDAKELSMPWPDFYKQS
                     FRNAEDSTWKYPKDADPNAWLDQQENLLSELSWGVMSRMMVNDKYNEHFKEHFSEYKR
                     LYKKEADNKLQNLLYGQVRQAAADSNEASFQEALELMKKAFPDEPDNAYYLNLYYCQS
                     TGQWDKCAAALQARIDDPDLEVGLGEINSMAWSLYESCEDRKVLEQALTWFDPFLEDM
                     ADYPAMDTYAALLFKTGRLDEAEKWAINAIDTGKADNMNVSDTEKLLEMIRQKNR"
     gene            complement(1923..2762)
                     /locus_tag="PK742_03360"
     CDS             complement(1923..2762)
                     /locus_tag="PK742_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQU75655.1"
                     /translation="MDLISYFSDIPSSHRALILAGGITFFWMVESIIPLFRFRYQKFR
                     HALPNLFFTITTVVVNLLFAFMIVRAADNAVVHQRGLLYLVHLPFWLFTLVGLMLLDL
                     IGAWLIHLVEHKVKWMWKFHVVHHSDRFVDTTTANRHHPVESMFRAVFTLLAVLVAGT
                     PIWLVMLYQSLSVVLSQFNHANITLPAWLDKSISWLIVSPNMHKVHHHYLQPLTDTNY
                     GNIFALWDRFFGTFAFIRDPKQELEYGIDLLMDEKESQQIDRLLAMPFKRYQPLKGSK
                     FSE"
     gene            complement(2795..3499)
                     /locus_tag="PK742_03365"
     CDS             complement(2795..3499)
                     /locus_tag="PK742_03365"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQU75656.1"
                     /translation="MKRLPGMFLSAVGLLTIALVTYSFINKQSSSLPEERSLEVDAYH
                     GLAVAGAFEVEITKGAQGPVQLSGAHLEDVEVKVKDGVLMIGCEKNCGQVQVNITVPD
                     LDRIDLAGSGKIHSKDALGGNSGLHMAVSGSGHIETNVQAEKVKAHISGSGKINCVGS
                     TQSVIVNISGSGQFDGSALKSRDAQVEVSGSGRTEVYASESAKADVSGSGRVMVHGNP
                     AEFKHSVSGSGKVEKM"
     gene            3593..5140
                     /locus_tag="PK742_03370"
     CDS             3593..5140
                     /locus_tag="PK742_03370"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQU75657.1"
                     /translation="MIANDGEKFKKVVSHAKEYGYIYPSSEIYDGLSAVYDYGPYGSL
                     LKNNIKEYWWKAMVQMHENIVGIDAAILMHPTTWKASGHVDAFNDPLIDNKDSKKRYR
                     ADVLIEDFMAKLDDKIHKEVDKARKRFGGSFDEAMFISTNNRVLELTDRKKQIGDRLA
                     KAMTDGNMEELRQIIVDCEIADPESGSRNWTEVRQFNLMFSTKLGSVADAADTIYLRP
                     ETAQGIFVNFLNVQKTTRQKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQYFVR
                     PGEEDNWYSYWKEHRMKWHKSLGTDDSMLKFKDHENLAHYAKAAVDIEFQFPFGFKEL
                     EGIHSRTDYDLSRHQEFSGKKLQFFDPELNQSYVPFVVETSIGCDRMFLAMLCNAYDE
                     EQVQDDTRTVLKLPPALAPVKVAVLPLVKKDGLPEKALEIFHQLRFSHQCQYDEKDSI
                     GKRYRRQDAIGTPYCITVDHQSLEDGTVTIRERDTMQQERVSVEEVQQVVNERVQMNL
                     LLKKLVS"
     gene            5137..5760
                     /locus_tag="PK742_03375"
     CDS             5137..5760
                     /locus_tag="PK742_03375"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HQU75658.1"
                     /translation="MKKYTVFMLVSLLTISAYSQQYSQDLLRLCNWMTGSFSSNQQST
                     VDTSYYDIRLHMEPIFTDRTDGCWFYVEQALASNQDRPYRQRVYHVYEGRQGTFTSDV
                     YEIDQAIRYTGAWKNEELLHAITMDQLELKDGCSVNLRWNLATGTYEGGTAEKTCPSE
                     LNRAAYATSQVMVLAGRIVSWDRGFDEAGEQVGGAEKGPYQFVRVHD"
     gene            5753..6151
                     /locus_tag="PK742_03380"
     CDS             5753..6151
                     /locus_tag="PK742_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75659.1"
                     /translation="MTDLRSMWWLSMLLCCSWFGCSDDPPVDDDFHASEDYNRSIQVE
                     VKHLYDMSTAADSLLTDVTILLFEDRDNFAFGGYPDAFRITDTTGKASFEFLSGSYYW
                     IRATHPLLGELEDSVSTPANTLSFVFLTYF"
     gene            complement(6152..6736)
                     /locus_tag="PK742_03385"
     CDS             complement(6152..6736)
                     /locus_tag="PK742_03385"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQU75660.1"
                     /translation="MKRIQLSLVLSLVIGMAACTDNAQQGKTREDHETSPENKENRVS
                     PATSTTADLHGNTVTVQYGSPRVKGRVIWGGLVPYGQVWRTGANEATTITFSQDVLIE
                     GQLLKADTYSLFTIPEADQWIIIFNLEEKQWGAFKYDQEQDALRMEVTPVIGESLTEN
                     MTFTLEPQDNGGVIRFQWEFLTIDIPFVNAPAAG"
     gene            6770..6901
                     /locus_tag="PK742_03390"
     CDS             6770..6901
                     /locus_tag="PK742_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75661.1"
                     /translation="MFFPPSNKPTDPDDPGFNPETNRQLLWFGLIALVIILLINLFR"
     gene            complement(6902..7066)
                     /locus_tag="PK742_03395"
     CDS             complement(6902..7066)
                     /locus_tag="PK742_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75662.1"
                     /translation="MNSGDWIQVGGLMFLVILAIAICWIWLHLNWYYALPITVLSGTT
                     VWWLHRQIPW"
     gene            complement(7063..8985)
                     /locus_tag="PK742_03400"
     CDS             complement(7063..8985)
                     /locus_tag="PK742_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQU75663.1"
                     /translation="MIAIQNLSLHFGGQTIFEGISCTLREGERVGLVGKNGAGKSTLL
                     RVLSGEQEVRNGQVVLPKGDTIGYLKQELSNLKGRTVYEETVSAFDEALRIEARLTAI
                     EKELEQHTDTESTTFHDLMEEMHELSHRFEIMDVGSVEKQVEQVLTGLGFDRNDLQRP
                     VETFSGGWQMRIELAKILLRKPEIVLLDEPTNHLDIESIQWLEQYLQTYPGAIVLVSH
                     DRSFLDNVTNRTIEIVNGKIYDYPVAYTDFTALRQERIELQSAAQRNQEKHIEHTEQL
                     IEKFRYKASKAKFAQTLIHKLEKMDRIEVDDEESASIQFVFPQPPRSGKLVVEASGLS
                     KSYGSKEVLRDLQFNIERGDRIALLGKNGEGKSTLSRIIASIEPYTGTCRLGHNVTVG
                     YYAQNQADTLKGDESVFDVIDQVATGEIRTKIRGLLGAFLFRGDAIYKKVSVLSGGEK
                     SRLALCRLLLQPSNLLILDEPTNHLDMRSKDVLKEALLRFDGTVILVSHDREFLQGMT
                     NRIFHVANLGIKEYLGDVHDYLESRKQETTGSIGQVSKPKKGQAEKADYIKRKEQEKN
                     QRKLERAVQQAEQEIHTLEKSIADMELKLKDPEFFKKLAHDDPFFAQYEAEKEKLEKA
                     MGVWERAAAELEALKP"
     gene            9044..9706
                     /locus_tag="PK742_03405"
     CDS             9044..9706
                     /locus_tag="PK742_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HQU75664.1"
                     /translation="MPVHRSTLQFLRQLEKNNNREWFQSNRKAYETARVNVLEVIATV
                     LDGMSAFDPGLAAVEPASTLFRINRDVRFSNDKSPYKTNFGAVLSSGGKKSPQAGYYL
                     HLEAGSNSIAGGGVWMPDKDRLAAIRQEIDYNLKEFESILEAKNFKKYFGELSDEKLK
                     TRPKGYAADHPGLEHLKQKSYVAFRGFSEAELQDKKFEKEVVRTMEALLPLNEFLNRA
                     MD"
     gene            9760..11616
                     /locus_tag="PK742_03410"
     CDS             9760..11616
                     /locus_tag="PK742_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU75665.1"
                     /translation="MKKVVSALCGCFIGACSYGQSITVYQDTIVCSNDTITLYALVDG
                     SYGTDEYTVQEVPYSPEPIGGISMTMTDDTYLGPYSIGFDFCFFGEIYTQFYICSNGW
                     ISFVVPGAGWATNWTPDGPIPSAASSVPKTAVMGPWTDWHTGLCTNCIYRETVGVAPN
                     RKTIITFEEVPLFSCTTYEGTFQFVLHETTNLVENHLWEVDVCPAWDLGIATQGIHNQ
                     TGTVAYTVPGRNATDWAATDESWQYVPSSITWYDAGTGLVVGTGDSIDVSPDVTTTYV
                     AEVTLCDGSTYTDSATVTIAAPFDIDSSVQQIACFGDDNGSVGLTVTGASGPITYSWS
                     SGETTSGISGLSPGIYTVAVEEEGGCVVNLEFEITEPPLLELNLAEYQDITCFDGNDG
                     YINMDPEGGTPVYTFFLNGSAGSDSNFVNLEAGDYEIIVQDMNGCTASFEQTLTQPVE
                     LEVDAGNNINIPFGGSGNINATTNSTGTVTVSWLPTEGLSCADCLDPDASPSFTTTYI
                     ITVTDEAGCVATDEITIQVILDFTVPNAFTPNGDGVNDWFGISADFLESVTLDIYNRW
                     GDLVFHADDQNTRWDGLLNGVEQEIGTYIFKVNAVTITGTELIKTGTVTLLR"
     gene            11826..13475
                     /locus_tag="PK742_03415"
     CDS             11826..13475
                     /locus_tag="PK742_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU75666.1"
                     /translation="MITRKTLIIALLLASTASFAQIESVVKDEWIPESRMEQHNEFKA
                     GDYAYPAKPRSKWNIGLSLGVPFVTGDVAADPFGGHDGPPMGVGLNIRKGWGYLVSVR
                     AHANYGVTYGQNYTPVTYEKNDRINGNFANDSSAASTGVDYLTTGTQYIPNFKNTTIS
                     GGIDFIFNLNNVNFHKAESRFLPYLFAGIGAMSYNVKVNALDADGNIYDYNTLIIDYR
                     DVADREPKLDDLMDDTYETQADVDGSEKGDDVKTLRFSGDFGAGLLWRLGEKGNFELG
                     VEHRLSWTGDDLLDGQQWELGGTQTSATDFYHFSALTVGVNIGKNAQQPLWEVNPMGF
                     IYSKLNEFDIANLLADADDDGVVDYLDREPNTPAGTPVDTHGVSLDSDKDGCPDSEDP
                     EPFSTPNMPIENCQNVFVTENRVNEIIDERLKGIDLASLGGGAGSNWYLPMIFFDLDK
                     SNIRPDAVASLASVADIMKQYPKLKVEVVGYADTRASENYNLKLSENRAKAAIEYLSS
                     KGIDQSRFTMKYEGESNNLIPNATRESEHQMNRRVEFHIVK"
     gene            complement(13655..14944)
                     /locus_tag="PK742_03420"
     CDS             complement(13655..14944)
                     /locus_tag="PK742_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75667.1"
                     /translation="MINSSPPPAWLRWVAYGMIGIPVWWLSYFVDRTDISSVWLGYFL
                     PFIPFLFLMRNASALSWKELTIAAILIRTGLLWSTPNLSDDLYRFQWDGRLQDLGISA
                     YAQAPEDIMAGNPDDPVLQQLFPHLNSPEYYSIYPPGAQGLFWLSMQDVPHSVFWLKC
                     MMLILEVLTLYLLPHLLTLMGKLPQQTGWYALNPLVLIELSGNIHLETGMIAGCLLSA
                     LLLWKQRYVPAGIAMGMALSIKFTPLMILPLLWRPLGFRNAWTYYLAAGITLAAMFLP
                     VTNIQDLGHIGSSIGLYFHTFEFNGSWFYLYRLSDPDAYWLRDIFRILFPLLTFSLII
                     RWSWKSNTEKLPVYVVLSLAAFLFLSGNVHPWYLTPLLAAAVFTRLQFPFVWAVLIPL
                     TYITYRTSAYTENYWLVTLEYLLVYTVLITDLKRSNG"
     gene            complement(14941..>15406)
                     /locus_tag="PK742_03425"
     CDS             complement(14941..>15406)
                     /locus_tag="PK742_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75668.1"
                     /translation="VDINFFRYASVFLIGSISIGFAYFIATRQGEDSARHTWGTFIRR
                     FPIFLAITMGLSLHNARAVFKGWLGQKSAFIRTPKFNIVGKEGIWKNKQYVPTKISAI
                     TYLEGLFCLYFMGGIFLAFYYGDYGLLPFHLMAAFGFGLIFYFSLKHSRYKA"
BASE COUNT         3979 a         3719 c         3816 g         3892 t
ORIGIN      
        1 gagcatacgt atggtgctgc tcatgatagg atcacttctg gtggtgctct tatttgccct
       61 gccggtactt ggcatgcagg cctcctggtt acagcaacaa ctcagcgacc cgtttgccat
      121 tgccaacctg aaggcagatg ccggatggaa ctggttcgat ctgctgcccg gactggtgct
      181 gctaacagga atgaccgccg gtttttatgc tatgcgcaac caactgctgt atgggctttt
      241 cgtcctgttt gtgtctgttt tattgacggg aaaaacgggt atggtcatgc tggctccaaa
      301 ggtggaagct tatacccaac gggcggccgt tgaattcttc agtcaacacc gtgacgagga
      361 tgcctacctg gctgctattg gttacaagac ctatgctgat gccttttatg gaaggatgca
      421 gccggcagga aagccccggc tggaagatta ctctgataaa aagcaaaagg cagactggga
      481 caactggcta ctggccggaa acattgacag accggtttat ttcattaccc gtatgggaaa
      541 ggaatcttac ctggaaagca aaccggatat acagaaattg tatgagaaga atggcttctt
      601 gatgtactac aggcctgtac caccaccggg aggcaacttg cagtagcctg gtcttggcag
      661 aagggcatca tagtgtcata ttaacatgca cgatagcttt gtttagcacg gattttgcga
      721 gatttgtaca tagtgaaaca accccccgtt ttatgaaaca actgatcatc ggccttttat
      781 tgtcggcgcc tttgatgctg gcagcccagg atgttcactt cttccaggga acctgggaag
      841 aggtcaatgc caaagccaaa gcagagaata aacccatcat ggtagatgcc tataccgtat
      901 ggtgtggacc ctgtaagatg atggataaga ccatgttcca tgataatgct gctgtagcgg
      961 atctgctgaa cagcagtgtc attccttaca agatcgaatg tgaacatgat ttcgggttga
     1021 cattcaacca gaaattccgg gtagccggtt atcccagtct gttgttcttc aacagcgacg
     1081 gacagctttt tgaccgtctg ttgggctatg atgacaatac agccaatttt gtatccacac
     1141 tgaaggaggt attgtccgga gatcagcagg atacctacgg atatgatgcg aaagagctca
     1201 gtatgccctg gccggatttt tacaagcaat cgttccgaaa cgctgaagac agcacctgga
     1261 agtatccgaa agatgcagac ccgaatgcct ggctggatca gcaggagaac ctgttgtctg
     1321 aactgagctg gggggtcatg tcacgcatga tggtgaatga taagtacaac gaacatttca
     1381 aggaacattt ttcagagtat aaaagattgt ataagaaaga agcagacaac aaattgcaga
     1441 atcttttata cggtcaggta agacaggccg cagccgacag caatgaagct tcatttcagg
     1501 aagctctgga gctgatgaag aaagcttttc cggatgaacc ggacaacgcc tattacctca
     1561 acctgtatta ttgccagtca accggacaat gggataaatg cgctgccgca ctccaggcaa
     1621 gaattgacga tccggatttg gaagttggcc tcggtgagat caacagcatg gcctggtcgt
     1681 tgtacgaatc ctgtgaagac agaaaggtcc tggagcaggc gctcacctgg ttcgatcctt
     1741 tcctggaaga catggcagac tatccggcca tggataccta tgccgctttg ctgttcaaaa
     1801 caggacgtct ggatgaagca gagaaatggg ccatcaatgc cattgatacc ggcaaggcag
     1861 acaatatgaa tgtcagtgat acagagaaat tgctggagat gatcaggcag aagaaccgct
     1921 gatcactccg aaaatttcga cccctttaaa ggctgatatc tcttaaaagg catggccagc
     1981 agacgatcga tctgctggct ttctttttca tccatcagca gatcgatgcc gtattccagc
     2041 tcctgtttcg gatcacgaat gaaggcaaat gtgccgaaga aacgatccca caaggcaaag
     2101 atgtttccat agttggtatc cgtgagcggc tgcagataat gatgatgcac cttatgcata
     2161 ttcggagaaa cgatgagcca gctgatactc ttatccagcc aggccggcaa tgtgatgttg
     2221 gcgtgattga actgcgatag cacgacactc agcgactgat acaacatgac cagccagatc
     2281 ggagtgcctg ccaccagtac cgcaagcagc gtgaatacag cacggaacat actttccacc
     2341 ggatgatgcc ggttggcggt ggtggtatct acaaagcgat cgctgtggtg caccacatga
     2401 aacttccaca tccatttcac tttgtgttcg accagatgaa tgagccaggc accgatcaga
     2461 tccagcagca tcagtcccac cagggtgaac agccagaagg gaaggtgtac caggtacagc
     2521 aatccacgtt gatgaaccac agcgttatct gctgcccgca cgatcatgaa agcgaacagc
     2581 aggttgacca ctaccgtagt gatggtaaag aacagattag gaagtgcgtg ccggaatttc
     2641 tgatatcgga aacggaacag cggaatgatg ctttccacca tccagaaaaa agtgatccct
     2701 cctgccagga tgagtgccct gtgcgaagag ggaatatcac taaagtaact gatcaggtcc
     2761 atggaagaaa attacaaact tcttccctga cctgttacat cttttctacc ttgcctgatc
     2821 ctgaaacact gtgtttgaat tcagcaggat tgccgtgtac cattacacgt ccgctgccgg
     2881 atacatctgc tttggcactc tcactggcat acacctcagt tctgccactg ccactcactt
     2941 ctacctgtgc atctcttgat ttcagggcac tgccgtcaaa ctgaccactg ccggaaatgt
     3001 ttactatgac agattgtgtg ctgccaacac agtttatctt tcctgagccg gaaatatgtg
     3061 ccttgacctt ttcagcctga acatttgttt caatatgtcc gctgccggac actgccatat
     3121 gtaacccact gtttcctccc agtgcatctt tgctgtgtat ctttccgctg ccggccaggt
     3181 cgatcctgtc cagatctggt actgtgatgt tcacctgaac ctgtccgcag ttcttttcgc
     3241 aacctatcat cagcacccca tctttcactt taacttccac atcttccaga tgcgccccgg
     3301 aaagctgtac aggtccctgt gcgcctttag tgatctccac ctcaaatgcc ccggcaacag
     3361 caagaccatg gtaggcatct acctccagag atctttcttc aggaaggctt gaactttgtt
     3421 tgttaatgaa actgtaggtc accagagcga tggtcagcaa tcctacagct gacaggaaca
     3481 taccgggaag tcttttcata gcttatttga tttgttcaaa tctaaggcat tgctccaaga
     3541 ccgtctgtta aatgatgtta tggtggcccg tgctataacg tatctttgcg ccatgatcgc
     3601 caacgacgga gaaaagttca aaaaagtggt cagccatgcc aaggagtatg gctacatata
     3661 ccccagcagt gagatctatg acggactgag tgccgtctat gattacggtc cttatggcag
     3721 tctgctgaag aataatatca aggaatactg gtggaaggcc atggtgcaga tgcacgaaaa
     3781 tatcgtaggc attgatgcag ccatcctcat gcatcccact acctggaaag cctcaggtca
     3841 cgtggatgca ttcaatgacc ccctcattga caacaaggat tccaagaagc gttatcgagc
     3901 cgatgtgctc attgaagatt tcatggccaa actggatgac aagatccaca aggaagtgga
     3961 caaggccagg aaacgttttg gcggaagctt tgacgaagcg atgttcatca gcaccaacaa
     4021 ccgggtgctt gaactcactg atcggaagaa acagatcggc gaccggctgg ctaaggcgat
     4081 gaccgatgga aacatggaag aattgcgcca gatcattgtg gactgtgaga tagccgatcc
     4141 ggaatccggc tcccgaaact ggaccgaagt gcgacagttc aacctgatgt tcagcaccaa
     4201 actgggctct gtggctgatg cagctgatac catctacctc cgccccgaga cagcccaggg
     4261 catttttgtc aattttctga atgtgcagaa gactaccagg caaaaagtgc cattcggcat
     4321 cgcacagatc ggaaaggcat tccgcaatga gatcgtggcc agacaattca ttttccgtat
     4381 gcgggaattc gagcagatgg agatgcagta tttcgtccgg ccgggcgaag aagataactg
     4441 gtacagctac tggaaagaac accgcatgaa atggcataaa agcctgggta ctgatgacag
     4501 tatgctgaaa ttcaaagacc acgagaacct ggcacattat gccaaagctg cggtcgatat
     4561 tgagttccag ttcccgttcg gattcaagga actggaaggc atccattccc gcaccgacta
     4621 cgatctgtca cgccaccagg agttctccgg aaagaaatta caattctttg atccggaact
     4681 gaaccagagc tatgtacctt tcgtggtaga gacatccatc ggctgcgacc ggatgttcct
     4741 ggccatgttg tgtaatgcat atgatgaaga acaggtgcag gatgataccc gcaccgtgct
     4801 gaaacttccg cctgcgcttg cacctgtcaa ggtggctgtg ctgccgttgg tcaagaaaga
     4861 cggactgcct gagaaggcgc tggagatctt ccaccagctg cgtttcagtc atcagtgtca
     4921 gtatgatgag aaggattcta tcggcaagcg ttaccgtcgt caggatgcta ttggcacacc
     4981 gtattgtatc acggtagatc accagagtct ggaagacgga acggtcacca ttcgcgaaag
     5041 agataccatg cagcaggaaa gggtgtctgt agaagaggta cagcaggtag tgaatgagcg
     5101 tgtacaaatg aatctgttat tgaaaaaact cgtatcatga aaaaatatac agtgtttatg
     5161 ctggtttctt tattgactat atcagcatac agccagcagt attcacagga tctgctgcgc
     5221 ctctgtaact ggatgaccgg ctccttcagc agcaaccagc agtcaactgt tgataccagc
     5281 tattatgata ttcggctgca catggaaccc atatttaccg accgcactga tggatgctgg
     5341 ttctatgtgg agcaggcatt ggcttccaac caggacaggc cctatcgtca gcgggtgtat
     5401 catgtatatg aaggccgtca gggaactttc acctctgatg tgtatgagat cgatcaggcc
     5461 attcgctata ccggtgcctg gaagaatgaa gagctgctgc atgccatcac catggatcag
     5521 ctggagctca aagacggctg cagtgtgaac ctgagatgga atctggccac tggcacctat
     5581 gaaggaggaa cggcagaaaa aacctgcccc agtgaactga atcgtgctgc ctatgctacg
     5641 agtcaggtca tggtactggc cggacgaata gtttcctggg accggggttt tgatgaagca
     5701 ggtgaacagg tggggggtgc agaaaaagga ccctatcagt tcgttcgggt acatgactga
     5761 tctgaggtct atgtggtggc tttctatgtt gctgtgctgc tcctggtttg gctgctctga
     5821 tgatcctcct gttgatgatg acttccatgc ttctgaagac tataaccgca gtattcaggt
     5881 ggaggtcaaa cacctctatg acatgagtac tgctgctgat agtctcctca cagatgtgac
     5941 catcctgtta tttgaagacc gtgataattt cgcctttggc ggatacccgg atgccttccg
     6001 cattacagat accaccggaa aggcctcctt tgagtttctc tcaggcagtt actactggat
     6061 cagggccaca catccgttgc ttggtgaact ggaagattct gtcagcacac cggccaatac
     6121 cttgtcattt gtgtttctga cctacttctg atcagcccgc agccggtgca ttcacaaatg
     6181 gaatgtctat ggtcaggaat tcccactgaa atctgatcac accgccattg tcttgtggtt
     6241 ccagggtgaa ggtcatattt tctgtgagtg attcacctat gaccggtgtt acctccatgc
     6301 gcagtgcatc ctgctcctga tcatatttga acgctcccca ttgtttttcc tccaggttga
     6361 aaatgatgat ccactggtct gcttcaggaa tcgtgaacag gctgtaggtg tcagctttaa
     6421 gcagctgccc ttctatcaga acatcctggc tgaaggtgat cgtggtggct tcgttggcac
     6481 ctgtgcgcca gacctgacca tatggtacca gtccgcccca gatgacacgc ccctttaccc
     6541 tcggacttcc gtattgcaca gttacagtat tgccatgaag atcagcagta gtggaggtgg
     6601 ccgggcttac ccggttctct ttattttcag gcgaagtttc atggtcttcg cgggtcttgc
     6661 cctgttgtgc attgtctgta caggcagcca tgccaattac gagtgacaat acgagcgaca
     6721 gctgaatacg tttcatcatc ttacttttgt ggtacaaagg tagtacggta tgttctttcc
     6781 gccatccaac aaaccaacag atccggatga tcccggattc aatcctgaaa caaacaggca
     6841 attgctatgg ttcggcctca tcgccctggt catcatactt cttatcaatc tctttcgatg
     6901 atcaccaggg aatctgtctg tgcagccacc agaccgttgt acctgataat acagtgatag
     6961 gcaaggcata gtaccagttc aggtgcagcc agatccagca gatagcgatg gccagtatga
     7021 ccaggaacat cagtccaccc acctgaatcc agtcgccgct gttcatggct tcagtgcttc
     7081 gagttctgcg gcagcccttt cccacacacc catggctttc tccagttttt ctttctcagc
     7141 ttcatactgt gcaaagaagg gatcatcatg cgccagtttt ttaaaaaact ccggatcctt
     7201 cagcttcaat tccatatctg caatagactt ctccagtgta tggatctcct gttcagcctg
     7261 ctgcactgca cgctccagtt tgcgctggtt tttttcctgc tccttgcgct tgatgtaatc
     7321 tgctttttct gcctgaccct tcttcggctt gctgacctga ccaatggagc ctgtagtttc
     7381 ctgtttcctg ctttccaggt agtcatgtac atcacccaga tattctttga ttcccaggtt
     7441 ggctacatga aagatcctgt tggtcatgcc ctgcaaaaac tcccggtcat gcgataccag
     7501 aatgaccgta ccatcaaaac gcaacagtgc ttccttgagt acatccttgc tgcgcatatc
     7561 gaggtggttg gtgggctcat ccagtatgag cagattggat ggttgcagca gcaaacgaca
     7621 aagtgccagt cgcgattttt cgccaccgga taacacactc acttttttgt aaatggcatc
     7681 gccacggaag aggaaggcac caagcaggcc tcttattttt gtacgtatct cgcctgttgc
     7741 aacctgatca atgacatcga acacactctc atcccccttc agggtatctg cctgattctg
     7801 ggcatagtaa cctaccgtaa cattatgtcc aaggcggcag gtaccggtgt agggttctat
     7861 gctggcaatg attcggctca gtgtggattt gccttctccg ttcttaccga gcagggcaat
     7921 gcggtcacct ctttcaatgt taaactgcag gtcgcgaagc acttcttttg atccgtaact
     7981 tttagataat ccactggcct ccacaaccag tttaccggaa cgaggaggtt gtggaaagac
     8041 gaactgtata gaggccgatt cttcatcgtc cacttcaatg cgatccattt tttccagctt
     8101 gtggatcagt gtctgggcga atttcgcctt gctggcctta tagcggaatt tttcaatgag
     8161 ttgttcggtg tgttcaatgt gtttttcctg gttacgctgg gcagcagact ggagttcaat
     8221 gcgttcctgt cgcagggcgg tgaagtctgt gtaggccacc ggataatcat agatctttcc
     8281 gttcacgatc tcgatggtgc ggttggtcac attatccaga aaactccggt catgcgatac
     8341 caggacgatg gctccgggat aggtttgcag atactgttcc agccactgga tactttcaat
     8401 gtccaggtgg ttggtaggtt catccagcag cacgatctcc ggttttcgca acaggatctt
     8461 tgccagttct atgcgcatct gccagccacc actgaaggtc tctaccggtc gctgcaggtc
     8521 gttgcggtca aaacccagac cggtaagcac ctgctccacc tgcttttcta cagagcccac
     8581 gtccatgatc tcaaaacgat ggctgagttc atgcatttct tccatcaggt cgtgaaaggt
     8641 tgttgattct gtatctgtat gctgttcgag ttctttttca atggcggtta gtctggcttc
     8701 gatgcgcagg gcttcatcaa aagctgatac agtttcttca tataccgttc tgcctttgag
     8761 gttgctcagt tcctgcttca gatagccaat ggtgtcacct ttcggcaaaa caacctgccc
     8821 gtttcgaact tcctgttctc cagacaacac acgaagcagg gtggacttgc ctgcgccgtt
     8881 cttccccacc agtccaaccc tttctccttc ccggagggtg cagctgatac cctcaaaaat
     8941 ggtctggccg ccaaaatgca ggctcagatt ctgaatcgcg atcatgctgc aaaggtattg
     9001 cagagatatg ggtagatttg tctgtcagtc aaatcgttcg tttatgcccg ttcatcgttc
     9061 tactctgcag tttctcaggc aactggaaaa aaacaacaat cgcgaatggt tccaatccaa
     9121 ccgtaaagcc tatgaaactg ccagggtcaa tgtgctggag gtcattgcca ccgtactgga
     9181 tggcatgtct gcattcgacc ccggattggc tgctgttgaa cctgcgtcca cactgttccg
     9241 catcaacaga gatgtacgat tttcgaacga taaatcaccc tataaaacca acttcggggc
     9301 tgtgttgagt tccggaggaa agaaaagccc tcaggccgga tattacctgc acttggaagc
     9361 cggcagcaac agcatagctg gcggtggtgt ctggatgccc gataaagaca ggctcgcagc
     9421 catcaggcag gagatcgatt ataacctgaa ggaatttgag tccattctgg aagccaagaa
     9481 ctttaaaaag tattttggtg aattgtcaga tgaaaaacta aagacgagac ctaagggtta
     9541 tgcagcagac catccgggac tggaacatct gaaacaaaaa agctatgtag ccttccgggg
     9601 tttttcagag gcagaactgc aggataaaaa gttcgaaaaa gaggtggtca ggaccatgga
     9661 agccctgttg ccactgaatg aatttctgaa ccgggcgatg gattgatctg acatccggct
     9721 gatgatccgg ctgtttataa tttgatatct ttggaaaaca tgaaaaaggt cgtatcggct
     9781 ctttgtggtt gctttatagg tgcctgttca tatgggcaat ctatcaccgt gtatcaggat
     9841 accatcgtct gctctaatga caccatcacc ttatatgcgc tggtagatgg ctcctatgga
     9901 accgatgaat atactgtgca ggaagtgccc tattcgcctg aacctatagg aggtatcagt
     9961 atgaccatga cggatgatac ctatctgggg ccctattcca tcggttttga tttttgtttc
    10021 ttcggcgaga tctataccca gttctatatc tgttccaatg gctggatcag ttttgtcgtg
    10081 cccggagccg gatgggccac caactggacc cctgacgggc ccattccgtc ggcagccagc
    10141 agcgtgccca agaccgcagt gatgggtccc tggaccgact ggcatacagg attgtgcacc
    10201 aactgtatct atcgtgagac agtgggtgtt gcacccaacc gtaaaaccat catcacattt
    10261 gaagaggtgc ctttattcag ttgcactact tacgagggta catttcaatt cgttctgcat
    10321 gaaaccacca acctggtaga aaatcacctg tgggaggtgg acgtttgtcc ggcctgggat
    10381 ctgggcattg ccacccaggg catccataac cagacaggta ccgtagctta cacagttccg
    10441 gggcgcaatg ccacagactg ggcagcaact gatgaaagct ggcagtatgt gcccagcagc
    10501 atcacctggt atgatgccgg caccgggctg gtggttggaa cgggcgatag tattgatgta
    10561 tcgccggatg taaccacaac ctatgttgca gaagtgacct tgtgcgatgg ctctacctat
    10621 accgacagcg ccacagtgac cattgctgct ccgttcgata tcgattcttc tgtacagcag
    10681 atcgcctgtt ttggcgatga caatggttct gttggtctaa cggttacagg tgcttccggc
    10741 cccattacct atagctggtc ttcaggtgaa actaccagcg gcatcagtgg tcttagcccg
    10801 ggtatttata ctgtagctgt ggaagaagag ggcggttgtg tggtgaacct ggaatttgag
    10861 atcacagagc cgccattact ggagctgaac ctggcagagt atcaggacat cacctgcttt
    10921 gatggaaatg acggctacat taatatggat ccggaaggag gaactcctgt ctataccttt
    10981 tttctgaatg gttctgccgg ttctgacagc aattttgtca atcttgaagc cggcgattat
    11041 gagatcatag tgcaggatat gaatgggtgc accgccagtt ttgagcaaac gctcacacag
    11101 cccgttgagc tggaagtgga cgcaggtaac aacatcaata ttccattcgg tggttcagga
    11161 aacatcaatg ccactaccaa cagcaccgga acagttactg tcagctggtt acccacagaa
    11221 ggactgagtt gtgcggactg tctggatccg gatgcgtctc catcctttac caccacctat
    11281 atcattacgg ttacagacga agctggttgt gtggccacag atgagatcac aattcaggtc
    11341 atactcgact tcaccgtgcc taatgcgttc actcccaatg gcgatggtgt taatgactgg
    11401 tttggaattt ctgcagattt tctggaatct gttacattag atatatataa ccgctggggt
    11461 gacctggttt tccatgcaga cgaccagaat acccgctggg acgggctcct gaatggggtt
    11521 gagcaggaga tcggcaccta tatctttaag gtgaatgctg tgacaattac cggaacagaa
    11581 ctcataaaaa caggtacagt taccctgctt cgctgatttt tgtaacacgg ttacctgatt
    11641 tgtaacgtaa aaaacatccg tttttttttg tgcacctcaa tttttcgcat agttttgctc
    11701 ccgcaaggct gtcctttggc ctgtaaacac aatttaatat gaagaattgc cttcatattt
    11761 atttggttta cagcaggtct ataggtagtt ttgtgccgaa ataagaattt ttaaacaaac
    11821 aagtcatgat tacccgcaag accttaatca ttgctctgct gctggccagc acggcatcat
    11881 tcgctcagat cgagtccgtc gtcaaggacg aatggatccc cgaaagcaga atggaacaac
    11941 acaatgaatt caaagcagga gactatgcgt atcctgcaaa accacgcagc aagtggaaca
    12001 tcggccttag cctgggcgtt ccgtttgtaa ccggtgacgt agcagctgac ccctttggtg
    12061 gccacgatgg tcctcctatg ggcgttggtc tgaacatccg taaaggatgg ggttacctgg
    12121 tatctgtgcg tgcacatgcc aattatggtg ttacctacgg acagaactac acccccgtaa
    12181 cctacgagaa gaacgacagg atcaacggca actttgccaa tgactcatct gccgcttcta
    12241 ccggtgtgga ttacctgacc acaggaaccc agtacattcc aaacttcaag aatactacca
    12301 tcagtggtgg tattgacttc atcttcaacc tgaacaacgt taacttccac aaagcagaat
    12361 ccaggttcct cccttacctg ttcgcaggta tcggtgccat gtcttacaat gtgaaggtga
    12421 acgcactgga tgctgatggt aacatttatg attacaacac cctgatcatt gactacagag
    12481 atgtagcaga tcgcgaacct aagctggatg acctgatgga tgatacctat gaaactcagg
    12541 ctgatgtgga cggaagcgaa aagggtgatg atgtgaagac cctgcgtttc agcggcgact
    12601 tcggagcagg tctgctctgg agactgggtg aaaaaggaaa ctttgaactg ggtgtggaac
    12661 acagactgtc ctggaccggt gatgacctgc tcgatggtca gcagtgggaa ctgggcggaa
    12721 cccagacctc tgcaactgac ttctatcatt tctctgctct gaccgttggt gtgaacatcg
    12781 gtaagaacgc tcagcagccg ctgtgggaag tgaacccgat gggcttcatc tactctaaac
    12841 tgaatgagtt tgatattgct aacctgcttg ctgacgccga tgatgatggt gtggtagatt
    12901 acctcgaccg tgagcctaac actcctgccg gtactcctgt tgacacccac ggtgtttctc
    12961 tggacagcga caaggatggt tgccctgaca gcgaagatcc ggaaccgttc tctaccccga
    13021 acatgccaat cgaaaactgc cagaacgtat tcgttactga aaaccgtgtg aacgagatca
    13081 tcgacgagcg cctgaaaggt attgacctgg cttctctggg tggcggcgcc ggtagcaact
    13141 ggtatctgcc tatgatcttc tttgatctgg ataagtcaaa cattcgccct gatgctgtgg
    13201 catctctggc aagtgttgca gacatcatga agcaatatcc taaactgaaa gtggaagtgg
    13261 ttggttatgc tgacacccgt gccagcgaaa actataacct gaaactctct gagaaccgtg
    13321 ctaaagctgc tattgaatac ctgagttcta aaggtatcga tcagagcagg ttcactatga
    13381 agtatgaagg tgagtcaaac aacctgatcc ccaacgcaac ccgtgaatct gaacatcaga
    13441 tgaaccgccg cgtggagttc cacatcgtga aataagacct attcatgatt tgttgataga
    13501 cggggtcccc caggggcccc gttttttatt gccttttttt acggtacacg acgctttatt
    13561 agaaagagag aagaaactgc tttgatggtc agatacacat tcagtattcg tgccacgcag
    13621 tccatccttt tgaggacgta gaacgttcag aatgctatcc gtttgatctt ttcagatctg
    13681 tgatcagcac agtatagacc agcagatatt ccagggtaac cagccagtaa ttttcagtat
    13741 aagccgaagt ccggtaggtg atataggtaa gggggatcag tactgcccat acaaagggaa
    13801 actgaagcct ggtgaacacg gctgctgcta ggagaggagt cagataccat ggatgaacgt
    13861 tacctgacag aaaaagaaat gcagccagcg acaggacaac atataccggc agcttttcag
    13921 tattgctttt ccaggaccac ctgataatga gggaaaatgt caacaggggg aatagtatcc
    13981 tgaaaatatc cctcagccaa taagcatccg gatcagaaag gcggtagagg tagaaccagc
    14041 taccgttgaa ttcgaatgtg tggaaataca aaccaatact actgcctata tgacccagat
    14101 cctgaatatt ggtaactggc aggaacatgg ccgccagtgt tatacctgca gccagataat
    14161 aggtccaggc atttctgaat cccaggggtc gccacagaag cggcaggatc atgagtggag
    14221 tgaacttgat gctgagcgcc attcccatag caattccggc gggcacatag cgttgtttcc
    14281 ataatagcag ggcactcagc aagcaacctg ctatcatacc tgtttccaga tggatattac
    14341 cactcagttc tataagtaca agggggttta gtgcgtacca gcctgtttgc tggggcagtt
    14401 tacccatcag tgtcaggaga tgtggcaaca ggtacagagt caggacttcc agaatgagca
    14461 tcatgcattt cagccagaat acggaatgcg gtacatcctg catggataac caaaaaaggc
    14521 cctgtgcacc tggcggatag atggaatagt attctggaga attcaaatgg ggaaagagct
    14581 gttgcaaaac gggatcatca gggttgcctg ccatgatgtc ttcgggagcc tgagcatagg
    14641 ctgagatgcc cagatcctgc aggcgaccat cccattggaa gcgatacagg tcatcactga
    14701 gattaggagt tgaccacaac agcccggtac gtatcagtat cgcggcaatg gtcaattcct
    14761 tccatgacaa agcggatgca ttccgcatca ggaaaagaaa aggaataaaa ggcaggaagt
    14821 aacctaacca gacagaagaa atatcagtcc ggtcaacgaa ataagaaagc caccagaccg
    14881 gaatcccgat cattccataa gccacccatc tgagccatgc agggggtggt gaggaattga
    14941 tcatgcttta taccgggaat gcttgagcga aaaatagaaa attaagccga atccaaatgc
    15001 agccatgagg tgaaaaggca gcagaccata atcaccgtaa tagaacgcca ggaaaatgcc
    15061 gcccataaag tacaggcaaa aaagtccttc caggtaggtt atggctgaga tcttggtggg
    15121 cacatattgc ttgttcttcc agatgccttc cttaccaact atgttgaatt tcggtgtacg
    15181 gatgaatgca gatttttgtc cgagccagcc tttaaatacc gctcttgcat tgtgcaggga
    15241 taaacccatg gtaattgcca gaaatatggg gaatcttctg ataaaggtgc cccaggtgtg
    15301 tcttgcactg tcttctccct gacgggtggc aataaagtag gcaaagccaa tactgatgga
    15361 tccgatcagg aaaacactgg catacctgaa gaagttgata tctaca
//
LOCUS       DAOMAX010000036        15244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_441_length_15244_cov_3.958128, whole genome shotgun sequence.
ACCESSION   DAOMAX010000036 DAOMAX010000000
VERSION     DAOMAX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_15244_cov_3.958128"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PK742_03430"
     CDS             complement(<1..737)
                     /locus_tag="PK742_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQU75669.1"
                     /translation="MFELIVYDTANRVATITLNRPEKRNAFSPQLVAELKEAFAAAEA
                     DDSVRVVVLAANGPAFSAGADLAYLQALQSNSYEENLEDSESLMALYRQIYMLDKPVI
                     ARVQGHAIAGGCGLATVCDFVLAVPEANFGYTEVRIGFIPAIVMVFLLQRISGRAARE
                     LLLTGKLIPAETAQQYGLVNKVIRAEDLDGAVEQLAKELAETTSPSAVRMTRQMLGQI
                     SDMSMDEALYFAAEMNAKARETDDCRKG"
     gene            complement(737..3121)
                     /locus_tag="PK742_03435"
     CDS             complement(737..3121)
                     /locus_tag="PK742_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU75670.1"
                     /translation="MKQVLILSFLFPLLVQAQDLKQTIRGNIIDRESKATLPGANVLL
                     YADTVFLSGTTTNEDGNFRLEGPVGRVDLVVRYIGYQDFFITNLVVSSGKEVVLQIEL
                     EESAQALDEVTITAFREGEVVNDMATVSVRSFTVEETDRYAGSRGDPARMASNFAGVQ
                     GADDSRNDLVIRGNSPIGVLWRLENLDIPNPNHFAIAGTTGGPVSIINNKYLSNSDFF
                     TGAFPAEYGNATAGVFDLQMRKGNDEQYEFSGQLGFLGTELFAEGPLSKENKSSFLVS
                     YRYSTLQLFESLNIQIGTEAVPRYQDGAVKLFFPLKNGSISLFGIGGKSNIDILVSDY
                     TSPEEVELYGDSDRDQYFGTSMAAAGINYGHTISDKTYGKLTLAYTWQENHADHELVF
                     RNADFAIDSIIPNMGYSYRDQKLAAIYNLNHKFNSRNSMKAGVTLEQIHLLHIDSNLD
                     LSTWVFDNRYDFDGSTYFIRGYAQWKYKFSDAFHMVAGLHAAWFTLNSNSRSLEPRIS
                     GQYTFASGHRITGGLGLHSQLLPTYQYTYQELQADGSYIRINEDIGFVRSMHAVAGYE
                     WPVGDHLHLKLETYYQFLYDVPVEVEASAFSLANEGSGFARFFPGPLENTGTGDNKGV
                     ELTLERHFADHYFYLLTGSLYDSRYVGSDGVERPTNFNGKYAANILAGREFRISQKHV
                     LSASTKVTWAGGRLTSPVDTAATLTAKELEFVDSLTNTIRLKDYFRVDLRVAYLINTG
                     KLTHELAIDLVNMLNIQNVLGLTYAPNPTDPTVSPVREEYQLGFLPIFYYKIDF"
     gene            complement(3199..3738)
                     /locus_tag="PK742_03440"
     CDS             complement(3199..3738)
                     /locus_tag="PK742_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75671.1"
                     /translation="MKQLFPVAMMALLISFSSCGKKGMSDELKGKLHSFETDWKATGE
                     SLANFGTTMNSTFASMDDMMKDKNTMDHAGMAADKMAVMEELHNSCSNIEGKMEMVRT
                     AYTSTMTGFTDDEAAYREWKTKVQKHEIKEGDAEAGINDFQVKLQAYKDNLDAWGNLV
                     TELNDKCTENCKAMDALMQ"
     gene            complement(3773..6379)
                     /locus_tag="PK742_03445"
     CDS             complement(3773..6379)
                     /locus_tag="PK742_03445"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQU75672.1"
                     /translation="MEVRKTYDPAGIEQKWYEHWQQAGYFHSEPDDRDPFTIVIPPPN
                     VTGMLHMGHVLNNTLQDVFVRRARMQGFNACWVPGTDHASIATEAKVVGMLRERGIKK
                     SDLSRDAFMEYAWEWKEKYGGIILQQLKELGCSCDWERTRFTMDPEYYDDVIDVFIDL
                     YNKGYIYRGLRMINWDPEAKTALSNEEVIYKEVRSKLYFVRYLLEDGSDSLTIATTRP
                     ETILGDTAICVHPDDERYSNWIGKKVLVPIVNRLIPVIADTYVDREFGTGALKITPAH
                     DPNDYEIGQRHGLEIIDMMNENGTVSEAAGILVGEDRFAARKKLVAMIEEAGQLVKIE
                     ELTNNVGYSERTDAVVEPRLSMQWWLDMKKVSGPALDAVMNGTVKLHPDKFINTYRHW
                     MEHVRDWCISRQLWWGHRIPAWYNENKDLVVAKTQEEAVAHFRAKGLSENGIIQDEDV
                     LDTWASSWLWPISVFNGMKDPGGKDINYYYPTDVLVTAPEILFFWVARMIIAGYEYRN
                     DRPFDHVYLTGIVRDKQRRKMSKSLGNSPDPLDLIHQYGADGVRMGMLLCAPAGNDIL
                     FDEKQVEQGRNFCNKLWNAFRLVSGWEVADADTPARNKAAMEWFDAKLQQTIVSVDDQ
                     FAQFRVSDALMSLYKLAWDDFCAWYLEFIKPAYGQPIDRATKERTISFFETLLRLLHP
                     IMPFVTEELYHLLQERASGDDVMMSSWPVAGQVDTTALRSGELAMEVISAIRDLRARN
                     NLSPKESLTLWYEGDLSSLGDFTDAIRHLANISNMEVLSAEASGTGFLSGGIKWMLES
                     GEGGDPAEQKAKLEEQLKYTRGFLESVRKKLSNERFVSNAPADVVEVERKKQADAEEK
                     IRLLEESLLSLN"
     gene            6469..7668
                     /locus_tag="PK742_03450"
     CDS             6469..7668
                     /locus_tag="PK742_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQU75673.1"
                     /translation="MKIGLIREGKFPPDSRVALTPEQCTEVMRTWPDINIVVQPSHGR
                     CYTDAEYGQAGITLQENLSDCDVLIGIKEVPVESLISHKTYLFFSHTIKEQPYNRKLL
                     QTLLKQQIRMIDYETLHDEEGNRVIAFGRWAGIVGAHNAFWTWSRRNGGFKLTRARDC
                     HDFAELKTHYSHLQLPPLRIVVTGTGRVARGSMEVMELLKIRQVTPEEFLTSTCQEPV
                     VVNLDSHHLFERISDGGYERAEFHRHPERYRSAMHPYLSKSDILVNGIYWNPHGPRLF
                     EKTDMHADDFSIQVIADISCDVDGSVPATVRTTTISEPVFGYNKYTDETTAPFESDTI
                     DIMAVDNLPNELPRDASAEFGRRMIEAVIPDLMAESSRMLDEATICNKGELQEAYLYL
                     SDFAGVR"
     gene            complement(7665..8267)
                     /locus_tag="PK742_03455"
     CDS             complement(7665..8267)
                     /locus_tag="PK742_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU75674.1"
                     /translation="MELNEHLSPRAREDYSLVEQARSGSRTAFAALFSRYKDSIYYMI
                     LKMVHNRDDADDLTIESFGKAFSNLEKYTPDYAFSTWLFKIASNNTIDFIRKKRMDII
                     SLDDDENSESQNLSNSVSSNVPDPEEQFIREQRASLMRDMMDTLNPKYRRLIQLRYFE
                     ELSYDEIAAELNLPLGTVKAQLFRAKELLYQVVRGVREKY"
     gene            complement(8258..9373)
                     /locus_tag="PK742_03460"
     CDS             complement(8258..9373)
                     /locus_tag="PK742_03460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU75675.1"
                     /translation="MDSFNIFLYIYAAVVFIQLLHHLFFYSRLAWGRTAKPSGQELPG
                     VSVIVCARNEDTNLKQHLPLLLRQDYPAFEVIVVDDNSDDGTVEYLYYLAEKEPLLKR
                     VKVGNVNKPMAGKKFPLTLGIKAAQYPVVLLTDADCAPASDQWIRTMMQGYQAHTEVV
                     LGYAPFHKLAGWLNKTIRFETFFAGMNYLSYAKAGMPYMGVGRNLSYKTGMFFRYGGF
                     ADHRELPSGDDDLFINKIARRKNTRVVTDSNSFMYSAPKETWTDWKYQKQRHLSTARY
                     YRPLHKFFLSLQPVTQILVYLLLPLAVWKDPAFWYVPAGLFLFRWIINRILYTSVMRK
                     LDEKDLVPFIEVFDLLQVFYYFRFFKAAVVKTRYRWN"
     gene            9486..10616
                     /gene="tgt"
                     /locus_tag="PK742_03465"
     CDS             9486..10616
                     /gene="tgt"
                     /locus_tag="PK742_03465"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQU75676.1"
                     /translation="MFFQLQSTDPGGKARAGLIKTSHGEIETPIFMPVGTAGTVKAVH
                     FDALKDSIDASIILGNTYHLYLRPGTGLLQQAGGLHRFINWNRPILTDSGGYQVYSLA
                     QMRKILDDGVRFQSHIDGSRHHFTPAGVMQIQREIGADIVMAFDECPPYPSDRKYAEK
                     SMHLTHRWLDACVQTMQETEPLYGYDQYLFPIVQGSTYEDLRKQSAEYIANSDCPGNA
                     IGGLSVGEPHDMLYGMTETVCQILPESKPRYLMGVGTPENLLECIHLGIDMFDCVMPT
                     RNARNGMLFTREGIINIRNKKWENDLDPIDTDATTEVSRQHSKAYLRHLFVSGEMLGP
                     MIASIHNLGFYLWLVRAAREQIVAGTFSSWKNKMVKKVSTRL"
     gene            10616..11746
                     /locus_tag="PK742_03470"
     CDS             10616..11746
                     /locus_tag="PK742_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQU75677.1"
                     /translation="MKKPGSILLSPFNRVLSRLDRYIIAKFFVTSFFTFAIITSIAVV
                     IDYSEKTDAFVKNKPSTNEILFDYYINFIPHINALLAPLLIFLAVIFFTSRLAYNSEI
                     IAMLNSGMSYRRFLRPYMICAVFSALLLLYANHWLVPVANKTRVAFEDQYVRAQKSYG
                     NDIHMRLDEHTLISLNRFKYSTGEGNQFALERYTGTGKDRKLVYKINADRIQHIPGVH
                     NWRFINYRIWKIDGSSESFTKGKQMDTILDMEPDRFEVNTIIKESLNFKELNDFMQEE
                     NLSGTGGIEAYEVESHRRTAAAISVIIMIVIGAIMGSVKVRGGIGLNIVAAIAMGSLY
                     VVFLQFATSFSIKGSLPPIIGANVPNFIYGAVALILFRRFNK"
     gene            complement(11749..13287)
                     /gene="dnaB"
                     /locus_tag="PK742_03475"
     CDS             complement(11749..13287)
                     /gene="dnaB"
                     /locus_tag="PK742_03475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQU75678.1"
                     /translation="MSENNPQTTKRTYQPRKNPADLSDLVYGKLPPQATDLEEAVLGA
                     FMIDRDAVTNAIEILREDSFYDDRHRLIFKAIRQLFEKTQPIDILTVTEELRKMGRLE
                     EAGGPYYITSLTSRVASAAHVEHHARIVSQKFIQRELIRISTGIVHDAYEDTTDVFEL
                     LDNAEQSLFNIADQNLRTSYVELDNLLASTIKSIEESRKNEDSLTGVPSGFTNLDRLT
                     GGWQKSDLIIVAARPGMGKTAFTLSLARNAAVDFNRPVAFFSLEMSAQQLVKRLISSE
                     AELPAEKIIKGQLAEHEWVQMVKKSGKLGDAKIFIDDTPALNIFELRAKCRRLKMQHD
                     IQMIMIDYLQLMTTSESSKIGNREQEISTISRALKSIAKELNVPIIALSQLSRKVEDR
                     GASKKPMLSDLRESGAIEQDADMVVFLYRPEYYNLDQDEDGNSMRGIAQVIIAKHRNG
                     SLDTVNLRFIGQYIKFAELDEFASTENSNLRPLSDFTGDTTAHITRRSRMDELDDDQD
                     EPPF"
     gene            complement(13519..14697)
                     /locus_tag="PK742_03480"
     CDS             complement(13519..14697)
                     /locus_tag="PK742_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQU75679.1"
                     /translation="MPNDLFEGVDYFRIDDLLTTEQILVRDTVRQFVKQEISPIIEEY
                     AQKAETPKQLIPGLAEIGAFGPTIPVEYGGGGLDNVCYGLIMQEIERGDSGIRSLVSV
                     QGSLVMYPIYTFGSEEQRRKYLPKLGSGEMIGCFGLTEPDHGSNPGGMVTNIKDAGDH
                     VILNGAKMWISNAPFADIAVVWAKDEEGIIRGLIVERGMEGFTTPETHGKWSLRASAT
                     GELVFDNVRVPKENIFPNVKGLKGPLSCLSTARYGISWGAMGAAMDCYDTARRYSMER
                     KQFGKPIGQFQLQQKKLAEMLTEITKAQLLAWRLGVLKDEGKATPAQISMAKRNNVDM
                     ALHIAREARQMLGGMGITGEYPIMRHMMNLESVITYEGTHDIHLLITGMDITGLDAFK
                     "
     gene            14769..15128
                     /gene="folB"
                     /locus_tag="PK742_03485"
     CDS             14769..15128
                     /gene="folB"
                     /locus_tag="PK742_03485"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQU75680.1"
                     /translation="MGKILLQGMRFKSHIGVYDYEQQYGNNFSVDVEVSGPQIDGEDD
                     QLVQTIDYARIYSIVQDVMGSHCHLIETACKSILERMVQEDLTGNHTVVRIHKLHPPL
                     GGEVDQVGIEMEWNKFN"
BASE COUNT         3734 a         3876 c         3623 g         4011 t
ORIGIN      
        1 cccttccggc aatcgtctgt ctccctggct ttcgcattca tctcagccgc gaaatacagc
       61 gcttcatcca tactcatatc agagatctga cccagcatct gcctggtcat tctgacagca
      121 gaaggactgg tggtctctgc caactctttg gccagctgct caaccgctcc atccagatcc
      181 tctgcccgga tcaccttatt gacaagtccg tattgctgcg ccgtttctgc agggatcagt
      241 tttccggtca gcagcaattc tctggcagct ctgccggaaa ttctttgtaa cagaaatacc
      301 atcacaatgg ctggtatgaa accgatgcgc acttctgtat aaccgaaatt cgcttcaggc
      361 acagccagta cgaaatcgca aacagtggcg agtccgcaac cacctgcaat ggcatgtccc
      421 tgcacccggg ctatgaccgg cttatccagc atatagatct gccggtataa ggccatcaat
      481 gattcagaat cttccagatt ctcttcatac gaattggatt gcagagcctg caggtaagcc
      541 agatctgctc cggcagaaaa agcggggcca ttagcagcca gtaccactac ccgtacactg
      601 tcatcagctt ctgctgctgc aaaagcttcc ttgagttctg cgaccagctg cggactgaac
      661 gcattgcgtt tctccgggcg gttcagggtg atggtggcta cccggttggc ggtatcgtac
      721 acgatcagtt caaacatcag aaatctattt tataataaaa tatgggtaaa aagcccagct
      781 gatattcttc tcttaccggg ctaacggtcg gatcagtggg gttgggagca taggtcagac
      841 caagcacatt ctgaatgttg agcatattga caaggtctat ggccagttca tgggtaagct
      901 tacctgtatt gatgaggtag gccacacgaa ggtccaccct gaaataatcc ttcaaccgaa
      961 tggtattggt cagcgaatct acaaactcca actctttggc tgtaagggta gcagctgtat
     1021 caactggtga ggtgagcctt cctcctgccc aggttacttt tgtgctggcg ctgagcacat
     1081 gcttctgact gatgcggaat tcccttcctg ccaggatgtt ggctgcatac tttccattga
     1141 agttggtggg tctttcaacc ccatcgctgc ccacataccg gctgtcatac aaagatcctg
     1201 ttaacagata gaaataatga tcggcaaaat gtctttccag agtaagttct actcctttgt
     1261 tgtcacctgt tccggtgttc tccagagggc cgggaaagaa acgggcaaag ccactgccct
     1321 cgttggcaag cgagaaagca gaagcctcca cctctaccgg tacatcatac aggaactgat
     1381 aataggtttc caacttcaga tgcaaatgat ctccaacagg ccattcataa ccagccacgg
     1441 catgcatact gcgcacaaat ccaatatctt cattgatcct gatatagctg ccgtctgcct
     1501 gcagctcctg gtaggtgtac tgataggtgg gcagtaactg gctgtgcaaa ccgaggccac
     1561 ctgtaatgcg atgacctgat gcaaacgtgt attgtccgga tatccgcggt tccagtgacc
     1621 tgctgttgct gttgagggtg aaccaggctg cgtgcaggcc ggctaccatg tgaaaggcat
     1681 cgctgaattt atatttccat tgtgcatacc cgcgaatgaa ataggtactt ccatcaaaat
     1741 cataccggtt atcgaacacc cacgtggaga ggtccagatt ggaatcaatg tgcaagaggt
     1801 ggatctgttc cagtgtgaca ccggccttca tgctgttcct gctgttgaac ttgtggttga
     1861 ggttgtagat agcagccagt ttctggtctc tgtagctgta tcccatattg ggaataatac
     1921 tgtctatagc aaaatctgca tttctgaata ccagttcatg atctgcatga ttctcctgcc
     1981 aggtatatgc cagtgtcagt tttccatagg tcttgtcgct gatggtatgg ccataattga
     2041 tgcctgctgc cgccatactg gtgccgaaat actggtcgcg gtcgctatcg ccatacagtt
     2101 ccacttcttc cggagaagta tagtcactta ccagaatgtc aatattgctc tttcctccta
     2161 ttccgaacaa ggagatgctg ccgttcttca gggggaagaa caattttacc gccccatcct
     2221 gataccgtgg tactgcttct gtaccgatct gaatattcag gctttcaaat aattgcaggg
     2281 tgctgtaccg gtaactgacc agaaagcttg atttattttc cttgctaagc ggtccttctg
     2341 caaacagctc cgttccgaga aagcccagct gtccgctgaa ctcatattgc tcatcattcc
     2401 cttttcgcat ctgcagatcg aacacccccg cagtggcatt gccatactct gccggaaaag
     2461 ctccggtaaa aaagtcagaa ttggagaggt atttattgtt gatgatggac acaggtccac
     2521 ccgttgtgcc tgcgatagca aagtgattgg gattcggaat gtccagattt tccaaccgcc
     2581 acaatacccc gatgggcgaa ttgcctctga tgacgagatc atttcggctg tcatctgctc
     2641 cctgaacgcc cgcaaaattg gaggccattc tggcgggatc gccgcgacta ccggcatacc
     2701 ggtcagtttc ttctacggtg aatgaacgca cggatacagt ggccatatca ttaaccactt
     2761 ctccttcacg gaaagcggtg atggtcactt catccaacgc ctgtgcactt tcttcgagtt
     2821 ctatctgtaa aacaacctct ttaccactgc tcactaccag gttagtgatg aagaaatcct
     2881 ggtagccgat atagcgcacc acaaggtcca ctctgccaac cggcccttcc agcctgaagt
     2941 tgccgtcttc attggtggtg gtgccgctca gaaataccgt atcagcatac aacaggacat
     3001 tggctcccgg aagtgtggcc ttgctttccc ggtcgatgat gtttccacga atggtttgtt
     3061 tgagatcctg cgcctgtacc agtagaggaa acaggaaaga caaaatcaat acttgcttca
     3121 taaaggcggt gtttccgcca taaatatgca acaaaaaagc cggcattgtg ccggcctttc
     3181 tgcatgtagt gttccgtatt attgcatgag ggcatccatg gccttacagt tctcggtgca
     3241 tttgtcgttg agttctgtta caagatttcc ccaggcatcc aggttatcct tgtaagcctg
     3301 cagttttacc tggaaatcat tgataccggc ttccgcatca ccttccttga tctcatgctt
     3361 ctgcactttc gtcttccatt cacgatatgc agcttcatca tcagtgaaac cggtcatggt
     3421 agatgtatat gctgtgcgaa ccatctccat cttgccctca atattggagc agctgttgtg
     3481 cagttcttcc atcacagcca ttttatcagc agccatgcct gcatggtcca tggtattctt
     3541 gtctttcatc atatcatcca tggaagcaaa ggtgctgttc atggtagtac caaagttggc
     3601 gagtgattcg ccggtggctt tccagtctgt ctcaaaactg tgcagcttgc ctttgagttc
     3661 atcagacata cctttcttgc cgcaggaaga aaaggaaatg aggagtgcca tcatggcaac
     3721 aggaaacaat tgtttcattg catatttatt taggttacaa aactaccact tgttaattca
     3781 gcgaaagcag gctttcttcc agcaggcgga tcttttcttc tgcatctgcc tgtttttttc
     3841 tttccacttc caccacatca gccggagcat tgctgacaaa gcgttcattg cttagttttt
     3901 tcctcacaga ttccagaaag ccccgtgtgt acttcagctg ttcttccagt ttggcttttt
     3961 gttcggccgg atcgccgccc tctccgcttt ccagcatcca cttgatacct ccagacagga
     4021 atcctgttcc ggaagcttca gcgctcagca cttccatatt actgatgttg gcgaggtgcc
     4081 tgatggcgtc tgtgaaatct cccagactgc tcagatcacc ttcataccag agggtgagag
     4141 attctttggg cgacagatta tttctggccc taagatcacg aatggctgag atcacttcca
     4201 tggccagttc tcctgaacgg agtgcagtgg tatccacctg accggctaca ggccagctgc
     4261 tcatcatgac atcatctcct gacgctcttt cctgtaacag gtgatacaat tcttctgtaa
     4321 caaaaggcat gataggatgc agaagccgga gcaaggtctc aaagaatgat atggttctct
     4381 ctttggtggc ccggtctatt ggctgaccat atgcaggttt gatgaattcg agataccatg
     4441 cacagaagtc atcccaggcg agtttgtaca gactcatcag ggcatctgat acccggaatt
     4501 gcgcaaactg gtcatcgacc gaaactatgg tctgctgcag tttggcatcg aaccattcca
     4561 tggctgcttt atttctggca ggcgtatctg catcagctac ctcccatccg cttaccaggc
     4621 ggaacgcatt ccagagcttg ttgcagaagt tcctgccttg ttccacctgt ttctcatcaa
     4681 acaggatgtc atttcctgcg ggtgcacaca gcagcatgcc catgcgcacg ccatctgctc
     4741 cgtactggtg gatcagatcg agtgggtcag gtgaattacc cagactcttc gacatctttc
     4801 tgcgttgttt gtcgcgaacg ataccggtca gatatacatg atcgaatggc cggtcgttgc
     4861 ggtattcata tccggctatg atcattctgg ccacccagaa gaagaggatc tccggtgcgg
     4921 ttaccagcac atcagtggga taataatagt tgatgtcttt cccgcccgga tccttcatgc
     4981 cgttgaagac ggagatcggc cagagccagc tcgaagccca ggtatccagc acatcttcgt
     5041 cctggatgat cccattttct gacagccctt ttgcacgaaa atgcgcaacg gcttcttcct
     5101 gcgttttagc taccaccagg tctttgttct cgttatacca ggccggaata cgatgccccc
     5161 accagagctg gcgactgata caccagtcac gtacatgctc catccagtgg cggtaagtat
     5221 tgatgaattt atcgggatgc agttttactg ttccgttcat caccgcatcc agcgcaggcc
     5281 cggatacctt tttcatatcc agccaccact gcatgctcag acgtggttca actaccgcat
     5341 ctgttctttc tgagtagccg acattattag ttagttcttc gatcttcacc agctgtccgg
     5401 cttcttcgat catggccacc agtttctttc tggctgcaaa ccgatcttct cccaccagga
     5461 tgcctgcagc ttcgctgacc gttccgttct cattcatcat atcgatgatc tcgagtccat
     5521 gacgctgccc gatctcatag tcgttcgggt catgtgccgg agtgatcttc agggcaccgg
     5581 ttccgaattc acggtccacg taggtatcgg caatgacagg gatcagtctg ttgacgatgg
     5641 gcaccaatac tttcttcccg atccagttgc tgtatcgctc atcatccgga tgaacacaga
     5701 tagcagtatc acccaggatc gtttccggac gggtagttgc aatggtcagg ctgtcgctgc
     5761 catcttccag caggtatctt acaaagtaca gtttggagcg cacttcctta tagatgacct
     5821 cttcgttgct cagtgctgtc ttagcctccg gatcccagtt gatcatacgc aacccccgat
     5881 agatatatcc tttgttgtaa aggtcaatga atacatctat gacatcatcg tagtattccg
     5941 gatccatggt gaacctggtt cgctcccagt cgcaggaaca acccagttct ttcagctgtt
     6001 gcaggatgat acctccgtat ttttccttcc actcccaggc atattccata aaggcatcac
     6061 ggctgaggtc gctttttttg atgccccttt cccgcagcat ccccaccacc tttgcttcgg
     6121 tggcaatgga ggcatggtct gtaccaggta cccagcaggc attgaacccc tgcatgcgag
     6181 ccctgcgcac aaaaacatcc tgcaaggtat tattgagcac gtgccccatg tgcagcatac
     6241 cagtcacatt gggtggaggt atcacgatcg tgaacggatc acggtcatcg ggctctgaat
     6301 gaaaatatcc ggcctgctgc cagtgttcat accatttctg ctcaatccct gcaggatcat
     6361 atgttttacg cacttccatc tgctgttctt agggcaaaaa taaggctttc ggtgcgctct
     6421 gccgaaggca attcagcaaa gccctctatc tttaccacca tggggagtat gaagatcggg
     6481 ttgatcagag agggaaaatt cccgcctgat tcccgggtgg cgcttacacc ggaacagtgt
     6541 acagaggtca tgcgcacttg gccagacatc aatattgtgg tacaaccttc tcacgggcgc
     6601 tgttacactg atgctgaata cggccaggca ggcattacac tgcaggaaaa tctgagtgat
     6661 tgtgatgtac tgataggcat caaggaagtt cctgtggaat cgctgatatc gcacaagaca
     6721 tatctgtttt tttcacacac tataaaggag cagccttaca accggaagtt gcttcaaacc
     6781 ctgctgaagc aacagatcag gatgatcgat tatgaaacgc tgcatgatga ggaagggaac
     6841 agggtgatcg ctttcgggcg atgggccggt atcgtggggg cgcataatgc cttctggacc
     6901 tggagccgga ggaatggggg attcaagctg acacgagccc gggattgtca tgattttgca
     6961 gagctgaaga cccattattc acatcttcag ttaccaccgc tgcggatcgt tgtaaccggc
     7021 accggccggg tagcccgcgg ttccatggaa gtgatggaac tgctgaagat caggcaggtt
     7081 acaccggaag aattcctgac cagtacatgt caggaacctg tagtggtaaa tctggactct
     7141 catcacctgt ttgagcgcat cagtgacggg ggttatgaac gggctgagtt tcaccgtcac
     7201 cctgaacgtt acagatcggc catgcaccca tatctttcca aatcagatat actggtcaat
     7261 ggtatatact ggaatccgca cggaccaaga ttgtttgaaa aaaccgatat gcatgccgat
     7321 gatttcagca ttcaggtcat tgctgatatt tcctgcgatg tggatggaag cgtgcctgct
     7381 acggtgcgca ctactaccat cagtgagccg gtttttggct acaataaata cactgatgaa
     7441 accaccgcac cttttgaatc agacaccata gatatcatgg ccgttgataa tctgccaaat
     7501 gaacttccca gagatgcctc agcagaattt ggccgaagga tgatagaagc ggttattcca
     7561 gatctgatgg cagagagcag ccgcatgctg gacgaagcta ccatctgcaa taagggtgaa
     7621 ctgcaggaag cttatctgta tctcagtgac tttgccggag tgcgttaata cttctctctg
     7681 acacctctca ccacctgata cagcaattct ttggcccgga atagctgcgc ctttacagta
     7741 ccaagtggca ggttgagttc tgcagcgatc tcgtcataag acaattcttc aaaatatcgc
     7801 agctgtatga gcctgcggta tttcggattc agcgtgtcca tcatatcacg catcaaactt
     7861 gcccgctgct cacgtatgaa ctgctcttcc ggatcaggta cattggaaga aacggaattg
     7921 gaaagattct ggctttcaga attctcatca tcatccagtg aaatgatatc catacgtttc
     7981 tttctgataa aatcaatggt gttattggac gctatcttga ataaccatgt actgaaggcg
     8041 taatcaggtg tatatttctc cagattactg aatgcctttc caaagctttc tatggtcagg
     8101 tcatcagcat catcacggtt gtgcaccatc ttcaggatca tataataaat ggaatccttg
     8161 tagcgggaga agagcgcagc gaaggcggtt ctgctgccgg agcgtgcctg ttccaccagc
     8221 gaatagtctt cgcgggcccg tggagacaag tgctcattca attccatctg taccgtgttt
     8281 tcactactgc agctttgaag aaccgaaagt agtaaaacac ctgcaggagg tcgaacactt
     8341 ctatgaacgg caccagatcc ttttcgtcca gttttcgcat cactgaggta tagagtattc
     8401 tgttgatgat ccacctgaac agaaaaaggc ctgccggaac ataccagaaa gccggatctt
     8461 tccagacggc aagcggcaat aagagataga ccagtatctg ggtgacaggc tggagtgaga
     8521 ggaagaattt gtgcagcggc ctgtaatacc tggcagtact cagatgacgt tgtttctggt
     8581 atttccagtc ggtccaggtc tctttgggtg ccgaatacat aaaggagttg ctgtcagtta
     8641 ccacccgtgt attctttctc ctcgcgatct tgttgataaa gagatcatca tcgcccgacg
     8701 gaagctcccg gtggtcagca aaacctccgt accgaaaaaa catcccggtc ttgtatgaca
     8761 ggtttctgcc tactcccata tagggcatcc cggctttggc ataagacagg taattcattc
     8821 ctgcaaaaaa ggtttcaaag cgtatggtct tattaagcca gcctgccagt ttatggaaag
     8881 gtgcataccc gagtactact tccgtatgtg cctgataacc ctgcatcatg gttctgatcc
     8941 attgatcaga tgcgggcgca caatcagcat ctgttagcag caccaccgga tactgtgctg
     9001 ccttgatacc cagagtaagc ggaaatttct ttcctgccat tggcttgttc acattgccta
     9061 ctttgacccg tttcagcaat ggctcttttt ctgccagata atagaggtat tctacggtac
     9121 cgtcatcaga attatcatct accacaatga cctcaaaggc cggatagtcc tgacgaagca
     9181 acagtggcag atgctgtttg agattggtgt cttcattccg ggcacatacg atcacagaaa
     9241 ctcccggcaa ttcctggccg gagggtttcg cagttctgcc ccaggccaac cggctgtaaa
     9301 agaacaaatg atgcagcaac tgtatgaaaa ccacagctgc atagatgtag agaaagatgt
     9361 tgaatgagtc catgaagacg cagcaaaaat acggcaattg acaaggccga agaactgcca
     9421 ctgacataca atcaacagcc tatgcacaac agcagactgc ctgattttca agtacctttg
     9481 ccggcatgtt ctttcagctg caaagcacag atccgggtgg aaaagccaga gccggtctga
     9541 tcaaaacttc ccacggcgag atcgagactc ccatcttcat gccggttggc acggccggta
     9601 ccgtgaaggc tgttcatttt gatgcgttga aagacagcat agatgcttcc atcattttgg
     9661 gaaacaccta tcatctctat ctgcgaccgg gaacaggact cctgcagcag gctggcgggc
     9721 tgcaccgctt catcaactgg aacagaccta tattgacaga cagcggagga taccaggttt
     9781 acagtctggc gcaaatgcgc aagatactgg atgacggtgt tcgttttcaa tcgcatattg
     9841 acggatcacg ccaccacttt acaccggccg gtgtgatgca gatccagcga gagatcgggg
     9901 ctgacatagt tatggcattt gatgagtgtc cgccctaccc atctgacaga aaatatgctg
     9961 aaaaaagcat gcatctcacc catcgctggc tggatgcatg tgtgcaaacc atgcaggaaa
    10021 cagaaccgct atacggatat gatcagtatc tgttccctat cgtacagggc tctacgtatg
    10081 aagacctgcg taagcaatca gcagaataca tagccaacag tgattgcccg ggtaatgcca
    10141 tcggcggact gagtgtaggt gaaccacatg acatgctgta tggcatgacc gaaactgttt
    10201 gccagatact tcctgaaagt aaaccgcgtt acctgatggg tgtaggtacg cctgaaaatc
    10261 ttctggaatg catccacctg ggtattgata tgttcgattg tgtcatgcct acccgaaatg
    10321 ccaggaacgg tatgctattc acacgggaag gcatcatcaa tatcagaaac aaaaaatggg
    10381 aaaatgacct ggatcccatt gatactgatg ctaccacaga agtgagcagg caacacagta
    10441 aagcatatct gcgtcacctg tttgtaagtg gcgaaatgct cggacccatg atagcgagca
    10501 ttcacaatct aggtttctac ctttggctgg tgcgggctgc ccgtgaacag atcgtggcag
    10561 gaactttcag cagctggaaa aataagatgg tcaaaaaggt cagcacccgt ttgtgatgaa
    10621 gaaaccaggt agcatattat tgtcgccttt caacagggtt ctatcacgcc tggatcgcta
    10681 catcattgcc aaattctttg taaccagttt cttcaccttt gccatcatta cctctatcgc
    10741 cgtggtgatt gactacagcg aaaagaccga tgcttttgta aagaacaaac catccaccaa
    10801 cgagatcctg tttgattact atatcaactt cattccccac atcaatgccc tgctggcacc
    10861 gttgctgatc tttcttgcag tcatcttctt tacatcccgt ctggcctaca attctgagat
    10921 catcgccatg ctgaacagcg gcatgagcta ccggcgtttt ctcaggccgt atatgatctg
    10981 tgctgtattc agcgccctct tgctgctgta tgccaatcac tggctggtac ctgtggccaa
    11041 caagaccaga gtagcttttg aagaccagta tgtcagggca caaaagagtt acgggaatga
    11101 tattcatatg cggctcgatg agcatacact gatcagtctc aacagattca agtattctac
    11161 cggcgaaggc aatcagtttg cgctggaacg atacaccggc acaggaaagg accggaaact
    11221 ggtgtataag atcaacgcag accgcattca acacataccc ggtgtacata actggcgatt
    11281 catcaactac cgcatctgga agatcgatgg cagttctgaa tcatttacca aagggaagca
    11341 aatggatacc attctggata tggagccgga tcggtttgaa gtgaatacca tcatcaagga
    11401 atcattgaac ttcaaagagc tgaacgactt catgcaggag gaaaacctta gtggtaccgg
    11461 tggtatagag gcctatgaag tagaatcaca ccgccgcact gcagcagcca tttcggtcat
    11521 catcatgatc gtgatagggg ccatcatggg ttctgtaaaa gtgcgtggag gtattggact
    11581 caacatcgta gcagcaattg ccatgggaag tctctacgtg gtatttctgc agtttgctac
    11641 ctctttcagt atcaaaggca gtctgccgcc tatcattgga gccaacgtac ccaactttat
    11701 ttacggagca gtagctctga tcctgttcag gcgattcaac aaataagatc agaatggcgg
    11761 ttcgtcctgg tcgtcatcca gttcatccat gcgtgatcta cgggtaatat gggcggtggt
    11821 atcgccggtg aaatcagaca gcggacgaag gttgctgttt tctgtcgaag caaattcatc
    11881 cagctcagca aacttgatgt actgtccgat gaacctaagg ttgaccgtat ccagtgaacc
    11941 attccggtgc ttggcgatga tcacctgagc tatgcctcgc atggagttac catcttcatc
    12001 ctgatccagg ttatagtatt ccggacgata caggaataca accatatcag catcctgttc
    12061 tatcgcaccg gattcacgca ggtcactcag cattggtttc ttgctggcac cgcggtcttc
    12121 cactttacga ctcaactgcg acagtgcaat aatgggtacg ttcagttctt tggctatact
    12181 cttcaacgcc cgtgagatgg tactgatctc ctgttcacgg ttacctatct tgctgctttc
    12241 agaagtggtc atcaactgga gataatcgat catgatcatc tgtatatcat gctgcatctt
    12301 caaccttcgg cacttggcac gaagctcgaa gatgttgagt gcgggtgtat cgtctataaa
    12361 gatcttggcg tctcccagct taccgctctt cttgaccatc tgcacccatt catgttcagc
    12421 cagctgaccc ttgatgatct tctcagcagg gagttcagct tcagagctga tgagacgctt
    12481 gaccagctgt tgtgcagaca tttccaggct gaagaaagca acaggtctgt tgaaatcaac
    12541 tgcggcattt cttgccagtg ataaggtgaa agcggtctta cccataccgg gtcttgccgc
    12601 tacgatgatc aggtctgatt tctgccagcc cccggtaagc ctgtccaggt tggtaaaccc
    12661 tgatggaacg ccggtcagag agtcttcatt cttcctggat tcttcgatgg atttgatggt
    12721 ggaagcaagc aggttgtcca gttctacata ggaggttcgc aggttctgat cagcaatatt
    12781 gaacaggctt tgctcagcat tgtccaggag ctcgaacaca tctgtggtgt cttcataggc
    12841 gtcgtgcacg atgccggtgc tgatgcggat gagttcccgc tgaatgaatt tctgagacac
    12901 gatgcgggca tgatgttcca cgtgtgctgc cgatgctacc cgactggtca ggcttgtgat
    12961 gtagtatgga ccgccggctt cttccaggcg ccccatcttc ctgagttctt ccgtaactgt
    13021 cagaatatct atgggctgtg tcttttcaaa caattgcctg atggctttga atatcagccg
    13081 gtgcctgtca tcataaaagc tgtcttcacg aagtatctct atggcattgg tcactgcatc
    13141 acggtcaatc atgaaagcac ccagaacggc ctcttccagg tcagtagcct gaggcggtaa
    13201 tttgccgtaa accaggtcag aaaggtcggc gggatttttc ctgggctgat aggtgcgttt
    13261 ggtggtttgt gggttgtttt cgctcactgt cttggttgct taaaaaaagg ttcattccaa
    13321 tttgccagga gggctgctga cgggaggtca aaagtactcg ctgaccattc tgtgcacaag
    13381 catttcggcc gaaaaaactc tgcacagata atcgtgcatg aacacatcac tgctgctcac
    13441 aagttacact tatgcaagcc aatttgtaaa cggaatatcc acacgcatca tgtgcatgtg
    13501 gacaagtggt tcacaagttt atttgaaggc atccagtccg gtaatatcca taccggtaat
    13561 cagcaggtga atgtcatgcg tgccttcata ggtgatgacc gattccaggt tcatcatatg
    13621 acgcatgatg gggtactcgc cggttatacc catgccaccc agcatttgcc tggcttcacg
    13681 ggcaatgtga agagccatgt ccacattatt ccttttggcc atagagatct gtgccggggt
    13741 agctttgcct tcatctttca gaactcccag tctccatgcc agcaactgcg ccttggtgat
    13801 ctccgtcagc atctcagcca gtttcttttg ctgcaactgg aactgtccga tcggtttgcc
    13861 gaactgtttt ctctccatcg aatatctgcg ggcagtgtca taacagtcca tggcggctcc
    13921 catggcaccc cagctgatgc cataccgggc agtgctcaga caggacagcg ggcctttcag
    13981 tccttttaca ttggggaaga tattctcttt cggaaccctg acattatcga aaacgagctc
    14041 gccggtggca gaagcccgaa gcgaccattt accatgcgtt tccggtgtgg tgaatccttc
    14101 catacccctt tcaacgatga gtccgcggat gattccttcc tcatctttag cccatacaac
    14161 tgcaatatca gcaaaaggtg cattggagat ccacatttta gccccattca atataacatg
    14221 atcgccggca tccttgatat tggtaaccat cccgccgggg ttagaaccat gatcgggctc
    14281 ggtcaggccg aagcaaccga tcatttctcc ggatccaagt ttgggaaggt atttcctccg
    14341 ttgctcttca gaaccgaaag tatatatggg atacatgacc agtgagccct gtacagacac
    14401 cagcgaacga ataccactgt cacccctttc gatctcctgc atgatgagtc cgtagcagac
    14461 attgtccagt ccgccgccac cgtattctac cggaatagta ggcccgaaag caccgatctc
    14521 agcaaggccg ggaatgagtt gtttgggtgt ttctgccttc tgggcgtact cctctattat
    14581 aggactgatc tcctgtttaa cgaactgcct gacagtatcg cgaaccagta tctgttcagt
    14641 cgtaagcagg tcatctatgc ggaagtaatc caccccttca aaaagatcgt tgggcatatc
    14701 attgatttga accgcaaaga tagaaatgat ggcttgtttt catctgcagt tggcgtagat
    14761 tagcagttat gggaaagatc ctgttacaag gcatgcgctt caagagtcat atcggagtat
    14821 atgactatga gcaacagtac ggcaacaatt tttctgtaga tgtggaggtg agcggccctc
    14881 agatcgacgg ggaggatgac cagctggtac aaaccattga ttatgcccgc atctacagca
    14941 tagttcagga tgtcatgggg agccattgtc atctgatcga aaccgcctgc aaatccattc
    15001 tggagcgtat ggttcaggaa gacctgaccg gcaaccatac cgttgtgcgc attcacaaac
    15061 tccatccacc tctgggcgga gaagtggatc aggtgggaat agaaatggaa tggaacaaat
    15121 tcaactgatg gtctgttgaa tagcacctga tgccgtatat ttgcgcctac cccgaataaa
    15181 caggtcggat ggccgagtgg ctaggcagag ctctgcaaaa gctcgtacag cggttcgaat
    15241 ccgc
//
LOCUS       DAOMAX010000037        15224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_445_length_15224_cov_4.796229, whole genome shotgun sequence.
ACCESSION   DAOMAX010000037 DAOMAX010000000
VERSION     DAOMAX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15224
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_445_length_15224_cov_4.796229"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..932
                     /locus_tag="PK742_03490"
     CDS             135..932
                     /locus_tag="PK742_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75681.1"
                     /translation="MFPHKSLLWLAIPLFALAGLSSCTKKEGCTDPNALNYDASAEKD
                     CCCEYATTFDLELHLHGYVGANALLEGNTYTINGVDTRLDLVQFYVSNIRVVDASGTE
                     FPAENYLLVNPEVNGYDAGAVEAGSYTALRFDIGIDSLTNHADPSVYPANDPLGPQFP
                     PMHWSWDAGYIFIRIDGMADQDLNGTPETAFEMHVGKDANRVAVEIPYAFSAEAGDAF
                     TFHLNVNWNNFFDGVDMTGDITTHTSDNLDLTSILVTNINGMFSLEE"
     gene            929..1954
                     /locus_tag="PK742_03495"
     CDS             929..1954
                     /locus_tag="PK742_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQU75682.1"
                     /translation="MTAAYLTKKQLNFWLPALLLWMSAGCAIDPDIPEPSYSVPIPEG
                     FPAIPYPDDNFPDAGRIALGKELFFDPVLSIDSTVSCNNCHLTQWAFADHAPISAGVE
                     GRLGFRNAPTLANVAWQPIMTMDGGSPTLEQQFYIPLEDFHEMAFNMVLLTDRLQADP
                     YYTDRFYQVFEDLPSPFTITRAIAAYERTIISGNSKYDQVMRGIANFTPSEQIGYDLF
                     FSDSLACSSCHSPPFFTNFEFLNNGLYETYYPDSGRARITVDPDDAGKFKVPTLRNIA
                     WTWPYMHDGSLPDLATVIDHYQNGGSGHRNQDPRVHPLHLNDEQKQDLILFLNTLTDD
                     DFLQQSD"
     gene            1978..3096
                     /locus_tag="PK742_03500"
     CDS             1978..3096
                     /locus_tag="PK742_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQU75683.1"
                     /translation="MRNKFWWTLPVLIWVLSGCEKDMPMVEPSPYTYDPTPYTLTKPV
                     GLPAFPTSDFGNPLTNEGVDLGRKLFYDPILSADSTLSCAGCHHIEFAFTDSDKVFSL
                     GIDGILGVRNSMPIFNLIYTGLDPAFGGFFWDGRALHLEDQALLPIQDPVEMHNTLEA
                     AVAAVQAQERYQKDFYEAFCTTTVTSDLIARALAQFMRTVVSGNSKFDQAFNAVPGVF
                     LTEQELEGWMLFNDNEGADCFHCHGINGGLFTDFLFRNNGLNDASTYTDFPDPGRGNV
                     TGDTLDYGKFKTPSLRNIALTAPYMHDGRFATLDEVLEFYSTGVHDTPFTDPFMQKAY
                     QGGVQLSDEEKEALKAFLMTLTDTSFANNPAWQNPFAE"
     gene            3106..3468
                     /locus_tag="PK742_03505"
     CDS             3106..3468
                     /locus_tag="PK742_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75684.1"
                     /translation="MPIDEHVPIDFDPRWLAVLQQVKEQFGKKPNMESLLFLIGINEL
                     GTLNTSFSKEQKQDLMHIAVCTLLEPEGYYESLGPDEDGWPHFALIKRFDVLSLEEQE
                     TLLKQNIIRYFERLKKEP"
     gene            3465..4193
                     /locus_tag="PK742_03510"
     CDS             3465..4193
                     /locus_tag="PK742_03510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQU75685.1"
                     /translation="MKHLLWAPFLLLAACTSNKTGEQAPVTDTSMLQPETEQTEPMDN
                     QPEQIVKISTSYGDMLIKLYNETPKHRDNFIKLVKEGYYNGLLFHRVMQEFMVQGGDP
                     DSRNAAAGARLGSGSPGYTIPAEFRKDLFHKKGALAAARQPDQVNPQKASSGSQFYIV
                     QGKPASDDELAMMGQRMGITFTEDQKEVYRTLGGTPFLDQNYTVFGEVISGMEVIDKI
                     AAVQTDANDRPREDIKMTITLEEE"
     gene            complement(4205..5101)
                     /locus_tag="PK742_03515"
     CDS             complement(4205..5101)
                     /locus_tag="PK742_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75686.1"
                     /translation="MKRILTLMIAFMIGTALFAQQEFTFTPAGKKSKTIATVMQKGDT
                     WILREGQGSVVIDYLPVNLPEEFKTDGQEVVFEGVLGEIPANVRLQGKPVEITLIKRL
                     YRAVPKDKTDDIGHTDGGDKKQPEAIENLRGVVMLVANTWLIEGERNGHTERFVPDNM
                     PEEFREEGLEVFVSGSAGYLDPNVRAMGRPFRITELTAVVEQKMDTTAIQEPMKDLFP
                     FQEAGRITAVEGVVKLFSSDPDIYIIETNNGTRRYLPAIIPDDFKVHNLRVLVTGVYG
                     EIPANVRLMGTPFEIEVIEEIK"
     gene            complement(5148..7145)
                     /locus_tag="PK742_03520"
     CDS             complement(5148..7145)
                     /locus_tag="PK742_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQU75687.1"
                     /translation="MSMLPSRYFFWLALLLLSLPSRAQESVLVRWVPENSGTPSYILA
                     ILHNTEAARYPLSNVPYEVFPLVNTVAFEWLEESYETEQAKEIMQLREELNLKRYLER
                     DDNIRYELFIINNLQENVEQYAGLMPFYTMQLFRDFLRGEGRTYQEELLYQYALEETK
                     PVLSMLSMRNIAEYMATVPSEQQADVLSRFVNDQDLFATYEHDKAGLYRNQSLTELGL
                     LVTGTEQPAWLQAMRTRVNRDLVAQMMELSAGQSVLYILDAERIGGPGGIVPLLLEEG
                     AEVSDMPFTLSSDAEIEEYYTGTNELQADQFPEYLLDNLSDRSGYAVRNRIIKVDELQ
                     AGELPPAAITDPFGDGYDYLGTDTSFISEWFELRSNDAYFSIRMPEKTKWEETLTESI
                     NGNIKSVQAQVNHARSDLFYSIGYTIYPPNFDPGIRADFFDDFVYRSIRKINGQLVTQ
                     RIISDPEYVGREFVVSVDDSFYVRSQIILRGNILYQILCGGPDNKPYAPYAEEFIRSF
                     RLDRGKATNWYTYLDQQISCQFPRQPLIQNQNYPTQYGQVQVKTFNTEDFDTQLSYFL
                     SINQYPDGYPVKGSKSFYNGLISDAERQYFGRAVSVTKVRKGRNKGVEAVLQLNNGKV
                     YRMHFFVVDKVLYQYLVGGTASAMESAGVDYFLDHFLISDQ"
     gene            complement(7201..8121)
                     /locus_tag="PK742_03525"
     CDS             complement(7201..8121)
                     /locus_tag="PK742_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HQU75688.1"
                     /translation="MPNEDFQQHLHPILQELELVRRDQLNRMAFILIALLVAVAVGML
                     LLIPTFIIVILFLSLPVYLLMFGTRRKRLDVRSEFRPRVMRVIADSLPEWDYDQHRFM
                     PRAVYDAASLNLPVPDVYEGQDLFYATVHGAPSSLSELLTRQQERSPDGSIQYTTLFN
                     GLLLSVQLPKAFSGIHYLAGTGDEQQVQDYLKRFGDINTFLPEPVQDDTCRIGEQVWL
                     STIPQEAVSLMRGPLGPVLRELSDKAASPLLLSCKGQHFCLAVPLPEKIMEPSVFRSL
                     LTTRHIVQYKELLETLLGIPALLTTPANNS"
     gene            complement(8144..8815)
                     /locus_tag="PK742_03530"
     CDS             complement(8144..8815)
                     /locus_tag="PK742_03530"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HQU75689.1"
                     /translation="MSLKRSVIAVATIMLVLFLDQLLKIYIKTNLQLGEEFGIFGLSW
                     ARIHFTENEGMAFGMTFGGNYGKLLLTVFRLIAVGFLGYYLTVLIKHKAPRSLIFSLS
                     LICAGAMGNIIDSVFYGVIFSDSIYQVASILPEGGGYAPLLHGHVVDMLYFPMFHGHY
                     PEWMPFLGGKYYMFFRPVFNLADSSITLGVLSILLFQRSIFGHAEKSSSAGSGQESEP
                     TDITS"
     gene            complement(8812..9462)
                     /locus_tag="PK742_03535"
     CDS             complement(8812..9462)
                     /locus_tag="PK742_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQU75690.1"
                     /translation="MVTSKATPKAVKEKPVQKEKPVVKQPVSRKKGAAKKTSKPVAEP
                     TDPNQEVITTSVTKSELARIKSQASEVSVKQQGRLVTFKKDEDTVVQKPLEKTRYSDK
                     ELTEFRDLINRKLEDAKSELKYLQEQISRTSELGDDSDSRFKGLEDGTSTAEREYLSQ
                     MASRQIQYISHLEKALIRIQNKTYGICRETGKLISKERLRAVPHATLSIEAKKNQG"
     gene            complement(10019..13369)
                     /gene="ileS"
                     /locus_tag="PK742_03540"
     CDS             complement(10019..13369)
                     /gene="ileS"
                     /locus_tag="PK742_03540"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQU75691.1"
                     /translation="MTMEAKLTFTELKQLNLPNIDKEILAFWDAENIFQKTILERQDA
                     TPFIFYEGPPSANGTPGIHHVLSRTLKDIFCRFRTMDGYLVERKGGWDTHGLPVELGV
                     EKLLGITKEDIGKTITVEEYNSICRREVMKYKDQWEEVTRIMGYWVDMEKPYVTFEND
                     YIETLWQLLQKLHTKGLLYKGYTIQPFSPAAGTGLSSHELNMPGAYREVKDTTIVAQF
                     KATGAEAEKLLGVTAEREVFFLAWTTTPWTLPSNTALAVGRKIVYVQVRTLNPYTGKP
                     VEVVLAKDRLYAYFKEEQKGQPLEQHEGWNKHLPWEPVAEFSGEALSGLRYEQLLPYA
                     QPESGDAFRVVTADFVSTEDGTGIVHIAPSFGADDFRTAQQNGIGSLTLVDRQGRFSE
                     DAGELAGRYVKNYTDDPEWENPDVFIAVKLKEENRAFRVEKYEHNYPHCWRTDKPVIY
                     YPLDSWFIRTTAVKDRLLELNRTINWKPRSTGEGRFAQWLENLVDWNLSRSRYWGTPL
                     PIWRTADGTEEICIGSVKQLTEEFSKAKEAGLNTDSDLKLVNGLLDIDLHKPYVDRIV
                     LVSATGQPMYREADLIDVWFDSGAMPYAQWHWPFENEETFRRSFPADFIAEGVDQTRG
                     WFFTLHVLGTMLFDSVAYRNVVSNGLVLDKAGNKMSKRLGNGVDPFDTIYKYGADAVR
                     WYMITNAQPWDNLKFDFDGITEVQRKFFGTLFNTCSFFGLYANVDAWRPGKDNISISD
                     RPELDRWILSLLHSLTRDVRQAYESYEPTRAGRLIELFVTEHLSNWYVRLSRRRFWKG
                     ELSADKNAAYHTLYTCLHTIARLMAPVSPFFAEFLYRQLRVQEFLGDAASVHLSAFPV
                     ADQAVIDSDLEETMQLAQDLSSTILALRKKVNIKVRQPLRKAMIPVLQEDVRDKIRHI
                     SDLVLAETNIKELSLMEDTAGVVTRKVKPDFKKLGPLLGAHMKEAATMLGSLSQERIA
                     ELERWSQITLEIAGKEFVIPADHVEIVSEDIPGWLVANAGNLTVALDIQLTPELVAEG
                     NAREFVSRMQKLRKDSQLEITDRIVVEVVADKDLWDSLLDFKNYICTEILADELVSAV
                     DNQDFVKLEINEREIRARVIKTEAYGKK"
     gene            complement(13366..>15224)
                     /locus_tag="PK742_03545"
     CDS             complement(13366..>15224)
                     /locus_tag="PK742_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQU75692.1"
                     /translation="ISGEILLFLLLAVIVTIVVLFILFRSWIAVVFPLLFIGSGVVFS
                     MGLMSLIGFKVTLLTGLIPSLLIVIGVPNSVYLLNKYHIEFRKHGNKVKALTVIIEKV
                     GYAMFFTNLTTAVGFGVFYFTRVQMLTEFGLITFISIVMTFLISIIIMPVIFSFLPKP
                     RKSDTNHLNNKLFHGIINKLVSWTFGHGRSVIAASIILVLVALPGVLMLRSAGYILDD
                     VSHRTRLYQDQQFFESEFNGILPFEIVIEKKQALTRIRDTIVNPVYHHETEDSVIALD
                     TVYVVRTDTSDNQVTSYATLSKIAELQTLLSQYSCFSTSLSILDGLKFARQAYYNGNP
                     RYYDLPDFERLSPNDARVGDFLKNTKQSALTRSVFTDKTGSKTRVSVQIADVGSDSMP
                     HIMEALRPRVDSIFDQEHYHVYFTGTSVVALEGYNYLIKSLLGSVSLALIIIAIIMTT
                     QFRSLRMLLLALLPNLLPLFVTAAIMGYFHIPLKPSTVLIFSVAFGIAVDYSIHFLAK
                     YRQELIRHSFNVRDTVRVALQETGMSMIYTSIILFFGFIIFTFSEFEGTKNLGILTSL
                     TLIVALFTNMLLLPSLLIFFDKKLEAMHKRRHARTTSYVDLISEDQTDNDVK"
BASE COUNT         3803 a         3769 c         3594 g         4058 t
ORIGIN      
        1 tgtgacagac gcttcatgaa cttcaaagtt accagtttta caaaaaactg accctgatat
       61 ctgtcatttg gtgacttact gcacgttatt ccggaatcag gtgttgtacc tttaagaacg
      121 ataaaaaaca aaagatgttc cctcacaagt ctctattatg gctggccatt ccattgtttg
      181 cgctggctgg tttgtcttcc tgcactaaaa aggaaggatg tactgatccg aatgcactca
      241 actatgatgc ttcggcagaa aaggattgct gttgtgaata cgccaccaca ttcgacctgg
      301 agcttcattt gcatggctac gtaggtgcca acgccctcct tgaaggaaat acctatacca
      361 tcaacggagt ggatacccgc ctggatctgg tgcagttcta tgtcagtaat atccgggttg
      421 tagatgccag cggtactgag tttccggcag aaaattacct gctggtgaat ccggaagtga
      481 atggatacga tgcaggagct gtggaagcag gatcatatac cgcacttcgg tttgatattg
      541 gtattgactc gctgaccaac catgcagacc cctctgtcta tcctgccaat gacccgctgg
      601 ggccacagtt tccacctatg cactggagtt gggatgccgg ttatattttc atacgcattg
      661 atggtatggc cgatcaggat ctgaatggca cccctgaaac tgcttttgaa atgcatgtgg
      721 gtaaagatgc gaatcgggtt gctgtagaaa taccctatgc gttctctgcc gaagcaggcg
      781 atgcgttcac tttccacctg aatgtgaact ggaacaattt ctttgatgga gtggatatga
      841 ccggcgatat caccacccac acatctgata atctggacct cacgtcgata ctggtaacca
      901 atatcaacgg catgttcagc ctggaagaat gaccgctgct tatctgacaa agaagcaatt
      961 aaacttttgg ctgccggcgc tcctcctctg gatgagcgcc ggttgtgcta tagatccgga
     1021 tataccggaa ccttcctatt ctgtgcccat tccggaagga tttccagcca ttccctatcc
     1081 ggatgacaac tttccggatg ccggcaggat cgcacttgga aaagagttgt tctttgaccc
     1141 ggtattgtca atagattcta ccgtatcctg caacaactgc cacctgacgc aatgggcctt
     1201 tgctgaccat gcaccgatca gtgcaggggt agagggacga ttaggttttc gcaatgctcc
     1261 taccctggcc aatgtagcct ggcaacccat tatgaccatg gacggcggaa gccctacact
     1321 tgaacagcaa ttctacattc cgctggaaga ctttcatgag atggctttta acatggtgct
     1381 gctaaccgac cgcctgcagg cagaccctta ctacaccgat cgtttttacc aagtctttga
     1441 agatctgcca tctcccttta ccatcacccg ggccatcgca gcctatgaac gtaccatcat
     1501 cagcggaaac agcaaatatg accaggtaat gcggggcatt gcaaacttca ccccgtctga
     1561 acagatcggg tatgacctgt tcttcagtga ttcactggca tgcagcagct gtcattcgcc
     1621 gccgttcttc accaattttg aatttctgaa caacggatta tatgaaacct attatcctga
     1681 cagcggaagg gctcgcatta cggtcgatcc ggatgatgcc ggcaagttca aagtaccaac
     1741 actaaggaat attgcctgga cctggcccta tatgcatgat ggcagtctgc ctgatcttgc
     1801 aactgtcata gatcattatc agaacggagg ttcgggtcat cgcaaccagg acccacgtgt
     1861 ccatcccctg catctgaatg atgaacaaaa acaggatctg atcctttttt tgaatacact
     1921 gaccgatgat gactttcttc aacaaagcga ttagcgagta tgcatacctt tgtattgatg
     1981 cggaataaat tctggtggac gctgcccgtt ttgatctggg tgctatccgg atgcgaaaag
     2041 gatatgccga tggtggaacc atcgccctac acctatgatc caacaccgta cacgctcacc
     2101 aagccggttg gacttcccgc cttccccacc agtgattttg gcaatccact taccaacgaa
     2161 ggagtagatc tgggcaggaa acttttttat gaccctatcc tgtctgcaga cagcacccta
     2221 tcctgtgccg gatgtcatca catcgaattt gcctttactg acagtgataa ggtattcagc
     2281 ctgggcattg atggcatatt gggtgtgaga aattccatgc ccatttttaa tctgatctat
     2341 accggactgg acccggcttt tggaggtttt ttctgggacg gtcgtgccct gcatctcgaa
     2401 gaccaggcat tgctgcccat tcaggatccg gtagaaatgc acaataccct ggaggcagcg
     2461 gtagcggccg tacaagcaca ggaaaggtat cagaaagatt tttacgaggc attctgcact
     2521 actaccgtaa cttctgatct gatagcaaga gccctggccc agttcatgcg cacagtggta
     2581 tctggaaact ctaaattcga tcaggcattc aatgccgttc cgggtgtatt cctgaccgaa
     2641 caggaacttg aaggatggat gttgttcaat gacaacgaag gggcagattg ctttcattgt
     2701 catggcatca atgggggtct gttcactgat tttctgtttc gcaataatgg gctgaatgat
     2761 gcttccacct acaccgattt tcctgatccg ggcagaggta acgtaacagg agataccctt
     2821 gattacggca aattcaaaac accgtctctt cgcaatatcg cactcactgc tccctatatg
     2881 cacgacggtc gttttgccac actcgatgaa gtactggaat tttacagcac aggagtgcat
     2941 gacacaccat ttacagatcc tttcatgcag aaagcttatc agggtggcgt gcagctgtct
     3001 gatgaggaaa aggaagcact aaaagctttc ctgatgaccc tcactgatac ttcttttgcc
     3061 aacaatccgg cctggcagaa tccgtttgca gaataacttt gcctgatgcc gatagatgaa
     3121 catgtaccta ttgatttcga ccccaggtgg ctggctgtgt tgcaacaggt gaaagaacag
     3181 tttggaaaga aacccaatat ggaaagtctg ttgttcctga tcgggatcaa tgaactggga
     3241 accctcaata cctccttcag caaagagcag aaacaagacc tcatgcacat agcggtctgt
     3301 actttgctgg aaccggaggg atactatgaa tcactagggc cggatgagga tggttggccg
     3361 cactttgccc tcatcaaacg ctttgatgtg cttagtctgg aagaacagga aacattgttg
     3421 aagcagaata ttatccgtta ttttgagcga cttaaaaaag agccatgaag cacttactat
     3481 gggcgccttt cttattgctg gcggcatgca ccagcaacaa aaccggtgaa caagcaccgg
     3541 taacagacac gtctatgctg caacctgaaa ctgaacaaac agaacctatg gataatcaac
     3601 cggaacagat cgtaaagatc agcacctctt atggtgatat gctgatcaag ctttacaacg
     3661 agacacccaa acacagagat aatttcatca aactggttaa ggaaggttat tataacggcc
     3721 tgctctttca ccgggtcatg caggagttca tggtacaggg tggtgatcct gacagcagga
     3781 atgcggcagc aggagcaaga cttggaagtg gaagtccggg ctataccata ccagctgaat
     3841 tcagaaaaga cctgtttcac aaaaaaggag ctcttgcggc ggcccgccag cccgatcagg
     3901 tgaatccgca gaaagcgtct tccggctcac agttttacat agttcagggt aagcctgcca
     3961 gtgatgatga gctggccatg atgggccagc gaatgggaat cacttttaca gaagatcaga
     4021 aggaggttta ccgcacatta ggtggtacac cgtttctgga tcagaactat accgtattcg
     4081 gagaggtgat ctcaggcatg gaggtcatag ataagatcgc tgccgttcaa actgatgcca
     4141 atgaccgccc gcgtgaagac atcaaaatga ccatcaccct ggaagaagag tgatcaggtc
     4201 atgatcattt gatctcttct atcacctcaa tctcgaatgg tgtacccatc agcctgacat
     4261 tggctggtat ttcaccatat actcctgtta ccagtacacg aaggttgtgc actttgaagt
     4321 catcagggat gatggcaggc aaatagcggc gggtaccatt gttggtctcg atgatataga
     4381 tatcagggtc tgaactgaat aatttaacca ccccttctac agcggtgatg cgacctgctt
     4441 cctgaaatgg aaataaatct ttcataggtt cctgaatggc ggtagtatcc attttttgtt
     4501 ctacaactgc tgtcagctct gtgatgcgaa agggtcttcc catggcccgg acattcggat
     4561 ccagataacc ggcgcttccg ctcacaaata cttccagtcc ttcctcccgg aattcttccg
     4621 gcatattgtc aggcacgaac cgctctgtat gcccgtttct ttcgccttct atcaaccagg
     4681 tgttagctac cagcatgacc acgccacgga gattttcaat ggcttcgggc tgttttttat
     4741 cgccaccatc tgtatggccg atatcatcgg tcttgtcttt gggaactgct ctgtataatc
     4801 ttttgatcag ggtgatctca accggtttcc cctggagcct gacattggca gggatctctc
     4861 ccaacactcc ttcaaagacc acttcctggc catctgtttt aaattcttcc ggcaggttga
     4921 ccggcaggta atcaatgacc acactgcctt gtccttcacg cagtatccag gtgtcgcctt
     4981 tctgcatgac agtagcaatg gtcttgcttt ttttgccggc aggcgtgaag gtaaattcct
     5041 gctgggcaaa aagtgcggta ccgatcataa aggcgatcat cagggtcaaa attcttttca
     5101 tagccaatgt ttttcaggta tataacagca caaattatgc cgaatgatca ttgatcactg
     5161 ataaggaaat gatccaggaa ataatccaca ccagcagatt ccatggcaga agccgtacca
     5221 ccaaccaggt attggtacag caccttgtca actacaaaga agtgcatgcg gtacactttt
     5281 ccgttgttca gctgcagaac tgcttccacg cctttgttcc ggcctttcct gaccttggta
     5341 accgagacag cccgcccaaa gtactggcgt tctgcgtcgc tgatgagacc attgtaaaaa
     5401 gacttactgc ctttaacagg atatccgtcc ggatattggt tgatgctcag gaaataggat
     5461 aattgggtat caaagtcttc cgtgttgaag gtctttacct gcacctgccc gtattgggtg
     5521 ggataattct ggttctgtat aagaggctgc ctcggaaact gacagcttat ctgctgatcc
     5581 aggtaagtgt accagttggt tgccttacct ctgtccaggc ggaagcttct gatgaattct
     5641 tcggcatatg gcgcataggg tttattgtca ggccctccgc agagtatctg atacaggata
     5701 ttaccccgca ggatgatctg cgagcgcaca taaaatgaat catcaactga gaccacgaat
     5761 tctctgccaa cgtactcagg atcagaaatg atgcgttgcg ttacgagttg tccgttgatc
     5821 ttgcggatac ttctgtacac aaaatcatca aaaaaatctg cccggatacc gggatcgaaa
     5881 ttagggggat aaatggtgta tccgatcgaa tagaaaagat ctgagcgggc atggtttacc
     5941 tgtgcctgca ccgacttgat gtttccgtta atgctttctg tcagtgtttc ttcccatttg
     6001 gttttttcag gcatgcggat ggaaaagtat gcatcgttgg aacgcagctc aaaccattca
     6061 ctgatgaaag aggtatcagt tcccaggtag tcataaccat cgccgaaagg atctgtaata
     6121 gctgccgggg gcaattcgcc ggcctgcaac tcatccacct taatgatcct gttgcgcact
     6181 gcatatccgc ttcggtcgct caggttatcc aacaggtatt caggaaactg atctgcctgc
     6241 agttcattgg ttcctgtata atactcttcg atctcggcat cagaactcag cgtgaaaggc
     6301 atatcgctca cttcagcgcc ttcttccagc agcaagggta caattccgcc tggccctccg
     6361 atgcgctcag catccaggat atacagcact gactgaccgg cagaaagttc catcatctgt
     6421 gccaccaggt cgcggttaac cctggtacgc atggcctgca gccaggctgg ttgttccgtg
     6481 ccggttacca ggaggccaag ctctgtcagt gactgattgc ggtataatcc ggctttatca
     6541 tgttcatagg tagcaaaaag gtcctgatca ttaacaaacc tgctgagcac atctgcctgc
     6601 tgctctgaag gtactgttgc catatactcg gcaatgtttc tcatgctgag catagacagc
     6661 accggtttgg tctcttccag tgcatactga tacagcagtt cttcctgata ggtacggcct
     6721 tcgccacgca aaaaatcacg aaacaactgc atggtgtaga agggcatgag ccctgcatac
     6781 tgctctacat tttcctgcag gttgttgatg atgaaaagct catatctgat attgtcatcc
     6841 cgttcaagat agcgtttcag attgagctct tcacgcaact gcatgatctc tttggcctgc
     6901 tcagtctcat aagattcttc cagccactca aaagcaactg tattgaccag cggaaacacc
     6961 tcataaggca cattggacaa cgggtatctg gcagcttctg tattatgcag tatggccagg
     7021 atgtaggatg gtgttcctga gttttcaggt acccagcgca ccagaaccga ctcctgcgca
     7081 cgggatggaa gagacagcag caacagggca agccagaaaa aatatctgga gggaagcata
     7141 ctcattaacg caaatgtacc gcaattatcg catgcacttg ttccacaatt accgcactga
     7201 tcaggaattg tttgccgggg tggtcaataa tgcgggaata ccaagtaacg tctcgagcag
     7261 ctctttatac tgaacgatgt ggcgggtagt caataaagag cggaatactg aaggttccat
     7321 gatcttttca ggcagaggta ccgccagaca aaagtgctga cctttacagc ttagcaacaa
     7381 gggggaagcg gccttatctg acaactcacg aagcaccggg cccagcggac cacgcatcag
     7441 gctgacagct tcctgcggga tggtactcag ccaaacctgc tcaccgatac ggcaggtatc
     7501 atcctgcaca ggctcaggca ggaaagtatt gatatctccg aaacgcttca ggtagtcctg
     7561 cacctgttgt tcatcacctg tacctgccag atagtgaata ccgctgaagg ctttgggtaa
     7621 ctgaacagac aacaacaggc cgttgaataa agtggtgtac tgaatgctgc catccggcga
     7681 acgctcctgc tgtctggtca acaactcact caagcttgag ggtgcaccat gaacggtggc
     7741 ataaaacagg tcctggcctt catatacatc aggcacaggc agattcagtg atgcggcatc
     7801 atatacggct ctgggcatga aacggtgctg gtcataatcc cattcaggca ggctatcggc
     7861 aatgacacgc atgacccttg ggcggaattc tgaccgcaca tccaggcgtt ttcttctggt
     7921 accgaacatg agcagataca ccggcaggct cagaaacagt atcacgatga tgaaggtagg
     7981 aatgagcagc agcataccca cagccactgc aaccagcaat gcgatcagaa tgaaagccat
     8041 tctgttcaac tggtctctcc ttacgagttc cagttcctga aggataggat gtaaatgctg
     8101 ttgaaagtct tcgtttggca tccggtctgt ttatggccag gcatcaggag gtaatgtctg
     8161 tgggttcgct ttcctgtccg gatcccgctg acgatgactt ttcggcatga ccaaaaatac
     8221 tgcgttgaaa gagcaggata gacaagactc ccagtgtaat ggatgaatct gccaggttaa
     8281 aaacaggacg aaaaaacata taatattttc ctcctaaaaa gggcatccat tcaggataat
     8341 ggccatgaaa catagggaaa taaagcatgt caaccacatg cccgtgcagt agcggtgcat
     8401 agccacctcc ttcgggcaaa atggatgcta cctgataaat ggaatcagaa aagatcactc
     8461 cgtaaaatac gctgtcaatg atattaccca tggcgcctgc acaaatgagt gacaggctga
     8521 agatcaggct gcggggagcc ttgtgtttga tcagaaccgt aagataataa cccagaaagc
     8581 caacagctat caggcggaac actgtgagaa ggagtttgcc ataattgccg ccgaaggtca
     8641 taccaaaggc cataccttca ttttcagtga aatggatccg tgcccatgac aggccgaaga
     8701 tgccaaactc ttcacccagc tgcaggttgg tctttatata gatcttgagc aactgatcca
     8761 ggaaaaggac cagcataatg gtagctactg caatgaccga acgcttcaag ctcaaccctg
     8821 gtttttcttg gcttctatgc tcagggtggc gtgaggaaca gcccgcagcc tttccttgga
     8881 tatcagctta cctgtttccc tgcaaatacc ataggtcttg ttctgaatgc gaatgagcgc
     8941 cttctcaagg tgagaaatat actggatctg gcgtgatgcc atctggctga ggtattctct
     9001 ttctgcagtg ctggtaccgt cttccagacc tttgaatctg gaatcactgt catcgcccag
     9061 ttcagatgtc ctgctgatct gttcctgcag atacttcagc tcacttttgg catcttccag
     9121 cttgcggttg atcaggtctc tgaactcggt cagctctttg tctgaatacc tggttttctc
     9181 caaaggcttc tgcactactg tatcttcatc ctttttaaag gtgaccagtc ttccctgttg
     9241 tttcacactg acctcgcttg cctgcgattt gatcctggcc agttcagatt ttgtaacgga
     9301 cgtagtgatc acttcctggt tcggatctgt aggctcagct accggttttg aggtcttttt
     9361 tgcggctcct ttctttcggc tgaccggctg ctttacaacc ggcttttctt tctgaacagg
     9421 cttctctttg actgctttgg gggttgcctt actggtaacc actgtaaccg gtttcttctt
     9481 ttcaggaaca gccttactga caactttttc agttgttgac tttttagtca ctgctttctt
     9541 cactgctttc ccggcatcag cttttttcac atttgtttta actggtgctg ccttttttgc
     9601 tgcggtctta ggggccgact tttttgcaat cgttttgggg gctgcttttt taattgcagc
     9661 cttgggggca gtggtctttc cggcagcctt ctttgttacc ttttttgcaa ctgttttacc
     9721 ggctgctgct tttttcggag cggctttggt aactactgct ttcttagcaa ccttttttgc
     9781 aacggtcttc ttggctgccg cttttttgac cactttctta gctgcagttt tgggagcagc
     9841 tttctttgag gttgttttac cggcaacaat tttctttact gcttttttag gagcagcttt
     9901 tttctttacc gctgatttgg ccggagcaga ttttttggca gccgatttct tcggtgcggt
     9961 cttttttaca gcagcctttt tgggtgccga ttttttaggg gcggctttct tagtgctctt
    10021 actttttgcc ataagcttcc gttttgatga ccctggcccg gatttctctt tcattgatct
    10081 ccagtttcac aaagtcttga ttatcaacgg cagaaaccag ttcatctgcc agaatttcgg
    10141 tgcaaatata gtttttaaaa tccagtaaag aatcccacag atccttgtct gcaacgacct
    10201 ccaccactat tctgtctgtg atttccagct ggctgtcttt acgcagtttt tgcatacggc
    10261 tcacaaactc ccgggcattt ccttcagcca ccagttcagg ggtcaactga atatccaacg
    10321 ctacagtcag gttacctgca tttgccacca gccagcctgg aatatcctct gatacgatct
    10381 ccacatgatc ggccggaatg acaaactctt taccagctat ttccagtgtt atctgactcc
    10441 aacgctccag ttcagcaatt cgttcctgac taagtgaacc gagcattgta gctgcctcct
    10501 tcatatgagc accgagaagg gggcccagtt ttttaaagtc gggttttacc ttgcgggtca
    10561 ctacaccagc agtatcttcc atcagactca gttccttgat gttggtttct gccagaacca
    10621 gatcagaaat atgcctgatc ttatctctca catcttcctg cagtaccggg atcatcgcct
    10681 tgcgtaaggg ctgacgaact ttgatattga ccttcttgcg caatgccagg atggtagaag
    10741 aaagatcctg ggcaagctgc atggtctctt ccaggtctga gtctatgaca gcctggtcag
    10801 caaccgggaa tgcagacagg tgtacggatg ctgcatcacc cagaaactcc tgcacacgca
    10861 attgcctgta caggaattct gcaaagaaag gagaaaccgg tgccatcagt ctggcaatgg
    10921 tatgcagaca ggtgtataaa gtatggtagg ctgcgttttt atcggccgat aattctccct
    10981 tccagaaccg cctgcggctg aggcgcacat accagttact gaggtgctcg gttacaaata
    11041 gttctatcag cctgccggcc ctggtaggtt cataagattc ataggcctgc cgcacatctc
    11101 tggtcagtga atgcaacagt gacaggatcc acctgtccag ttccggccta tctgagatgc
    11161 ttatgttatc cttacccgga cgccaggcat ctacattggc atacaagcca aagaaggaac
    11221 aggtattgaa cagggtgccg aagaatttcc gctgtacctc cgtgatgcca tcaaaatcaa
    11281 atttcaggtt atcccagggt tgcgcattgg tgatcatata ccagcgcacc gcatcagcac
    11341 catacttgta aatggtatcg aatggatcaa caccgtttcc aagacgcttg gacattttat
    11401 tgcctgcttt atcgagtacc agcccgttgg aaaccacatt acgataggct actgaatcaa
    11461 acagcatagt acccagcaca tgcaatgtaa agaaccaacc acgggtctga tctacgcctt
    11521 cggcaatgaa gtctgcagga aaagaccgtc tgaaggtctc ttcattttca aagggccagt
    11581 gccactgtgc gtagggcatg gcaccgctgt caaaccagac atcgatcagg tctgcctcgc
    11641 gatacatggg ctgtcctgtt gctgacacca atacaatgcg gtcaacgtat ggcttatgga
    11701 gatctatatc cagcaatccg ttgaccagtt tcagatcact gtctgtattc aatcctgctt
    11761 cctttgcctt actgaattct tctgtaagtt gcttcacaga accaatacag atctcttcag
    11821 taccatcagc agtgcgccag atgggcaggg gtgttcccca gtagcgggag cgactcagat
    11881 tccagtcaac cagattctcc agccattgtg caaaacgtcc ttcaccggtg cttctgggct
    11941 tccagttgat ggtgcggttc agctcgagca accggtcttt gaccgcagtg gttctgatga
    12001 accaggaatc gagcggataa tatatgaccg gcttgtctgt tctccagcaa tgcgggtaat
    12061 tgtgttcgta tttctcaacc cggaaagccc ggttctcttc cttgagtttt acggcaatga
    12121 acacatccgg attttcccat tcaggatcgt ccgtgtaatt ttttacgtaa cgtccggcca
    12181 gctcgccggc atcttcagaa aatctaccct gcctgtccac cagggtcagg cttccgatac
    12241 cattttgctg tgctgtgcga aaatcgtcgg caccaaagct cggtgcaatg tgcacgatac
    12301 cggtaccatc ttccgtgctg acgaagtctg ccgttactac ccggaaggca tcgccgcttt
    12361 ccggttgggc atagggcaat aactgttcat agcgaagacc actcaatgct tcaccgctga
    12421 actcagctac cggttcccag ggcaggtgct tgttccagcc ttcgtgctgt tccaatggct
    12481 gacccttctg ctcttccttg aagtacgcat acaacctgtc tttggccagt acaacttcca
    12541 ccggtttccc ggtatatgga ttcagcgtgc gcacctgcac gtacacgatc ttcctgccaa
    12601 ctgccagagc agtattggaa ggaagggtcc acggagtggt ggtccaggcc aggaagaaaa
    12661 cttccctttc ggcagtaaca cctaaaagct tttcagcttc agcgcctgtg gctttgaact
    12721 gcgccacgat ggtggtgtct ttcacctccc ggtaagctcc gggcatgttg agttcgtggc
    12781 tgctcagacc ggttcctgca gcgggcgaga atggctggat agtatagcct ttgtacagga
    12841 gacctttggt atggagtttt tgcaacaact gccagagggt ctctatgtaa tcgttctcaa
    12901 aggtgacata gggtttttcc atatccaccc agtaacccat gatccgggtt acctcttccc
    12961 actggtcttt gtacttcatg acctcacggc ggcagatact gttgtattct tctaccgtga
    13021 tggtcttgcc tatatcttct ttggtaatgc caagcagttt ctctacaccc agttccacag
    13081 gcaggccgtg ggtatcccat ccgccttttc tttctaccag gtaaccatcc atggtgcgga
    13141 agcgacaaaa gatgtctttg agagttcggg acagcacgtg gtgaataccg ggagtgccgt
    13201 tggcagaagg cggaccttca tagaaaataa aaggtgttgc atcctgcctt tcaagtatgg
    13261 ttttctgaaa tatgttttct gcatcccaga aagccaggat ctccttgtcg atattcggca
    13321 ggttcagctg tttcagttca gtgaaggtca gttttgcttc catggtcatt tcacatcatt
    13381 atctgtctga tcttctgaga tcagatctac ataggaggtc gttctggcat gtctgcgttt
    13441 atgcatggcc tccagtttct tatcaaagaa aatgagcagg ctgggcagca gcaacatatt
    13501 ggtgaacagg gcgactatca gtgtgaggct ggtcagaata cccaggttct tggtgccttc
    13561 aaattcagag aaggtgaata tgatgaagcc gaagaacagg atgatggagg tatagatcat
    13621 gctcatacct gtttcctgca gggccaccct aacggtatct cttacgttga aactatgtct
    13681 gatcagttcc tgtcggtatt tggccaggaa atggatggaa taatcgaccg ctattccgaa
    13741 tgccacactg aaaatgagta ctgtggatgg tttcagcgga atatgaaaat atcccatgat
    13801 ggcagctgtc acgaacaaag gcagcaggtt aggcagcaag gccagcaaca gcatgcgcaa
    13861 tgaccgaaat tgggtggtca tgataatggc aatgatgatc aatgccagag aaacagagcc
    13921 cagcaggctc ttgatcaggt aattatatcc ttccagagcc accacactgg tgccggtaaa
    13981 atagacatgg tagtgctcct gatcaaaaat ggaatctact ctgggtcgca gggcttccat
    14041 gatatgcggc atagaatcag agcccacgtc tgctatttgc acacttacgc gggtcttgct
    14101 gccggtcttg tctgtaaaga cacttctggt cagtgcagat tgttttgtat tcttcagaaa
    14161 atcacctacc ctggcatcat tcggactcag ccgttcaaaa tccggcagat cataatacct
    14221 tgggtttccg ttgtaatatg cttgccgggc aaactttaaa ccatccagaa tagacaaact
    14281 ggtactgaaa caggaatact gactgagcag tgtttgcagt tctgcgatct tgctcagggt
    14341 ggcatagctg gtcacctggt tgtcagatgt gtctgtgcgc accacataga ccgtatccaa
    14401 tgcaatgaca ctatcttctg tttcatgatg atagaccgga ttcacgatgg tatcgcggat
    14461 tcgggtaagt gcctgttttt tttcgatgac gatctcaaaa ggaagaatac cattgaattc
    14521 agattcaaag aattgctggt cctgatagag tcttgtgcgg tgacttacat cgtccagtat
    14581 atatccggca gaacgcagca tcagcacacc gggcagtgcc accagtacca gtatgatgct
    14641 ggctgcaata acactgcggc cgtgaccaaa ggtccagctt accagcttat tgatgatacc
    14701 atgaaacagt ttgttgttca ggtgattggt atcacttttt ctgggtttag ggagaaagct
    14761 gaaaatgacc ggcatgataa tgatggagat caggaaggtc atcacgatgc tgataaaagt
    14821 gatgagcccg aactcagtaa gcatttgcac cctggtaaag tagaacacac caaagcccac
    14881 tgcggtggtc aggttggtga aaaacatggc atagcccact ttctcaataa tgaccgtcag
    14941 ggctttgacc ttgttgccat gtttcctgaa ttcaatgtgg tacttgttga gcagatacac
    15001 tgaattaggc accccgatca caatcagcag actgggtatg aggcctgtaa gcaaggtcac
    15061 cttgaatcct ataagtgaca taagccccat gctgaacacc acaccgcttc ctatgaaaag
    15121 caaagggaac acgaccgcta tccatgaccg gaacaaaatg aacagcacta cgatcgtaac
    15181 aatgactgcc agcagcaaaa acaacaggat ctcacctgaa atgg
//
LOCUS       DAOMAX010000038        15223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_446_length_15223_cov_4.385483, whole genome shotgun sequence.
ACCESSION   DAOMAX010000038 DAOMAX010000000
VERSION     DAOMAX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15223
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_446_length_15223_cov_4.385483"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PK742_03550"
     CDS             <1..834
                     /locus_tag="PK742_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQU75693.1"
                     /translation="GAGLPFIPPWLREDLAEWDLAAGNKLDEIIRLEDLRGVVHNHST
                     WSDGRNTLREMAEACMKLGYEYLVISDHSKTAVYAGGLEVEKVIQQHKEIEQLNVELA
                     PFRIFKSIESDILNDGSLDYPDDILQQFDLVIASVHQNLRMDPEKATSRLMAAVENRY
                     TAILGHMTGRLLLSRPGYQPDHKKIIDACVANGVAIEVNANPYRLDMDYHWIPYAMEK
                     GLLMCINPDAHSVEGIADMHWGVCAARKGGLLRAQTLNALPLEAFEKWVSDRRAAKGI
                     D"
     gene            846..1694
                     /locus_tag="PK742_03555"
     CDS             846..1694
                     /locus_tag="PK742_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HQU75694.1"
                     /translation="MRHYLSLIKFSHTVFALPFALIGFFLALQTLDGPFPWTDLLLVL
                     GCMVFARSAAMAFNRYLDSDIDALNARTAVREIPKGVISRPQALIFVILNSIAFIVCA
                     GLLNELCLYLSPVALLVILGYSYTKRFTWLCHFVLGLGLSLAPVGAWLALTGQFAWLP
                     IVFGLIVLTWVSGFDILYALQDETFDREHHLHSVPEWLGRRKALSLSIIVHVLTAALV
                     IVAGQIADGGTWYWIGAGFFTAMLIYQHMLVKPDDISRVNLAFATTNGMASVAFGILV
                     IADILF"
     gene            1694..2104
                     /gene="ruvX"
                     /locus_tag="PK742_03560"
     CDS             1694..2104
                     /gene="ruvX"
                     /locus_tag="PK742_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQU75695.1"
                     /translation="MGRILAIDHGKKRCGIAVTDPMRMIGSPLTTVDTEKVFEFLHDY
                     FQKETVDLLLVGYPTNIQGEATDATPLVDRFIEQCRKKFPSLELRTWDESFSSRKAMQ
                     TLVQSGVKKKDRRQKGALDQVSAAVILQEYLQNL"
     gene            2106..2693
                     /gene="def"
                     /locus_tag="PK742_03565"
     CDS             2106..2693
                     /gene="def"
                     /locus_tag="PK742_03565"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQU75696.1"
                     /translation="MILPIVAYGNPVLKKVAEPIGSDYPELKELLSNMWETMYNGRGV
                     GLAAPQIGLGIRIFLVDSEQLVKENEEAEEDEELYRVTQGIKKAFINPRIIEETGEPW
                     NYEEGCLSIPDVRVKISRKELVRIRYVNEQFEEIEETYDGMNARIIQHEYDHLEGILL
                     TDHMSPLKRSLLKGKLDNISKGKIDVNYRMRFLQR"
     gene            complement(2690..3307)
                     /locus_tag="PK742_03570"
     CDS             complement(2690..3307)
                     /locus_tag="PK742_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU75697.1"
                     /translation="MKRFSFLVLLLLPLSLFAQDKRILQFSGLVMTSDSLRAIPLVNI
                     YSKTSGTGTFTDWKGFFSLVAREGDTIIFSAIGFETAEAVIPDSLPEDRYSMVQLMTG
                     DTIFLAETVIFPWPSREDFRETFLYADIPADQYEIAAKNLEREKLKELGEAYGYDADA
                     NQDYYTKQIARELYYAGQNPPIRIFDVFAWKAFIEAWQRGDFKNH"
     gene            complement(3365..3601)
                     /locus_tag="PK742_03575"
     CDS             complement(3365..3601)
                     /locus_tag="PK742_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75698.1"
                     /translation="MQIKKTIGTSLLISVIVLHVTVRIMAKDAYVISTADYSSTKEVT
                     PAAINYVSADQSDIKMYQSALGWNLRPQQNPIPY"
     gene            complement(3871..4368)
                     /locus_tag="PK742_03580"
     CDS             complement(3871..4368)
                     /locus_tag="PK742_03580"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75699.1"
                     /translation="MKNLFTFLLTIATVSLFAQGSNTGTGGTTINPELENLGGFKPDI
                     NNFNPNNSGVFGPFGNADATEGPSHVANYTDRQESEGAGPRTLATVTYSLYPNPATDY
                     VVIELSEKITGTLQLLNIVGQVIQSAPIDQSLIRLELRDVEPGVYFISIMSGDEKIVK
                     QLKIQ"
     gene            4627..6081
                     /locus_tag="PK742_03585"
     CDS             4627..6081
                     /locus_tag="PK742_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75700.1"
                     /translation="MYQNKAIIILRTFTPQEMRQMDDFVLSPFFNKNQKVADLYLVIR
                     DSHPDFDAPVIERQRLFEAVFPGSAYDEQKLRYLLTDLTKLLEEYICWKAVEEEVLFK
                     YHLLLYSYRQRGLDKPFMSVLKQAEKTLEDWPRRDVSHAFYEYLIEEDKYKFSALQKE
                     HLPETNLQKVVDNLDKYFILNKMRYSAEIINNRNVVAINYRLFLYEEIMNHLRNNPLD
                     HVPAAKIYYNVILTLTEPENKLHYSNLLLLLEEHRNIFSREELFDMYVYAKNFCIRKI
                     NNGHTEYMKELFGLYQIILENRIIFRENYLSQRDYKNIIYLGLRLEEFGWVKSFIEEY
                     NDQLDPRYRKNAYTYNMAYYHFFLGEYDETLTMLRSVEFTDVYYHLDSKSLLLKTYYE
                     LGETEAFLSLVEAFKIYIKRNKLIPVNQKNNYNNLIRFVTKLYKWKLSPRNNLQEIAS
                     EIERTKPIADVIWLRKKLEEAEQIAAKHSGSWRK"
     gene            6175..9027
                     /gene="gcvP"
                     /locus_tag="PK742_03590"
     CDS             6175..9027
                     /gene="gcvP"
                     /locus_tag="PK742_03590"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQU75701.1"
                     /translation="MSHTDRFVDRHVGPSMDDIREMLAVIGEPSLEALIDKTIPQQIR
                     LKRDLELSAPLSEYEYLRALDQKASQNKVFRSYIGLGYYNTITPSVIRRNILENPGWY
                     TQYTPYQAEIAQGRLEALLNYQTMVSDLTGLEIANASLLDEGTAAGEAMIMFFHHKNK
                     KDITAPKFFVADDCFPQTIDVLRTKAAPMGIELVIGSAESFRPADDYFGALLQYPGVN
                     GDVRDYTSLIEACHQHEVWVTLAADIMSLAMLKTPGEMGADCAVGNSQRFGVPMGFGG
                     PHAAYFATKDEFKRVIPGRIIGVSIDRRDKPALRMALQTREQHIRREKATSNICTAQA
                     LLAIMAGMYAAWHGPVGIRNIAGNIHRNAGRLQETLAALGFEVVNKNWFDTLTIRHSG
                     QADAIRKEAVSMGINFYYDAEVVRISLDETVSEQDLKDILTVFEKVSGKQAKLSDEST
                     GLKADALRKTEYLTHPVFNRYRSESEMMRYIKGLENKDLSLTTSMIPLGSCTMKLNAA
                     TELLPITWPAFSAMHPFVPADQVKGYLEIIAELEKDLCEITRFTSCSLQPNSGAQGEY
                     AGLMVIRAYHLDRGDTQRTKVLIPASAHGTNPASAVMAGMDVVVVASDANGYIDMDDL
                     RAKAEQHKNELGALMVTYPSTHGVFEETIIEVCEIVHQYGGLVYMDGANMNAQVGLTS
                     PAHIGADVCHLNLHKTFAIPHGGGGPGMGPICVNDKLAPYLPGHPLVKTGGDKAITPV
                     SAAPFGSASILLISYGYIKMLGGEGCTMSTKAAILNANYLKSRLEGAYDILYVGQSGR
                     VAHEFIIDFRKWKHTIGLEVEDVAKRLMDYSFHAPTVSFPVAGTLMVEPTESESREEL
                     DRFCEAMLSIREEIRALEEGKADASDNVLKNAPHTLADVIVSDWTHAYTREEAVYPVP
                     YLRERKFWTSVNRINNTHGDRNLICVCPPMESYMEA"
     gene            9168..10457
                     /locus_tag="PK742_03595"
     CDS             9168..10457
                     /locus_tag="PK742_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HQU75702.1"
                     /translation="MNPDIHFVSPQEALSCIHSGDTVFIHGSAQTPVGIMPYLAERKN
                     ELEHVQLVFISLYGDIILDRPEYDDAFDLNAMFVSGSVRNAVAEGRADYIPVFLSEIP
                     LLFQRDIIPLDVAIVQVSPPDKHGYCSLGVSVDIARTAVNKARHVIAVINPNVPRTHG
                     DSLIHSIRFDSMVWMDEPLQEVTFGDKVGEEELAIGKHIAGLIEDGSTLQMGIGAIPD
                     AVLRFLTNHKNLGAHTEMFSDGLIPLFESDVINNRYKVIEPNRTVTGFAVGSKKLYDY
                     VDDNPAFAFLDIDFVNDPAVISRNPKVVAINSCIEVDLTGQVVSDSIGTRQYSGVGGQ
                     MDFIRGAAMCPEGKPIIALSSRTKKGQSRIVNTLKAGAGVVTTRAHVHYVVTEYGVAY
                     LFGKNLRQRAKALINISHPDDREELHQACFERFRIFV"
     gene            10526..12196
                     /locus_tag="PK742_03600"
     CDS             10526..12196
                     /locus_tag="PK742_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQU75703.1"
                     /translation="MQRRKFIRQVSLAVPALFTSRALGAWPSFLSAGATGQVLIIGAG
                     SAGLYAARQLADQGISVTILEASSQHGGRVSPLDGFADFHVEAGAEFVHGKGNTTGDP
                     PSFLWSSINDYNSDLLQEYGAYKEIYDVSGVPKLSPPYWDMQLEQCWQFYLDMYTYSG
                     PDVLMSDYLFDTYGIDESHPYWHFYEAWIGAEFGTSIKRIGMNSIAISELLWKTGSKD
                     FLLDTGYRDLLDTLFFGPVLSDIQYNKVVTGIDTGGDQVSVQCEDGSVFTADKVIVTV
                     PLTILKQNSITFEPPLPALKTSAIEIIGMDAGMKIFLKFSENFWGDDVQDITIDGYST
                     FIWAPGMGKTGATDHVLVCFVMGERAEYLSSLDAGAIDVVMSELDSLFDGAASPLLLD
                     HFIQDWATEPFIGGAYSYPSPGTYMSESESARIDLAAPVDCKLFFAGEATSLQHPATV
                     HGALESGARVAEEVLACPFPTDLNSPQYQDFQAYYENGLIYVEWLPFFTAGEELMLVN
                     AQGQMVGKWPIDNSRSSSELDVRHLPRGVYILYLINGAKRNSRLLIFS"
     gene            12205..14043
                     /locus_tag="PK742_03605"
     CDS             12205..14043
                     /locus_tag="PK742_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQU75704.1"
                     /translation="MQKIVLTACLGLSVLSAQAQLNVDLLYQNDYPENMSNIWGWTAP
                     GGEEYVILGSFDGTYLIDITDPSSPDELQFIDGNNSTWRQIAVWDHYAYVVNESGGGL
                     LCIDLSGLPGTAAYAFSDADGAGLTTAHQVWADENGYVYVFGSNLDGGSSVILNANAD
                     PMDPPMVGSTDTWYVHHGFARGDTLYESNIYQGWFSVWDVSDKSMPVLLATQETPGTF
                     THNVVPSDNGQFLFTTDEKSNGSLAAYDISDLSDIQYQSEFRANPGTSSIPHYAWYFD
                     GFVVTAWYKDGVIITDATHPEFMIKTGYYDTHPGSGSGFSGAWGCYPYFPSGTLVVSD
                     ISEGFFVLHPTYVHASFVEGTITDSDSGLPVFGATASLLTLSGTTVSSSLDGGYITGA
                     VADGIYTLEVSKAGYATASIPVSLSAGVTLAEDILLEPVTATCESPVILGASSITSSS
                     AYLDWTNVGADQYSVIYKKVGGSGTIHTTTATNYLATGLESCSTYKWAVRASCPDGSK
                     KKSVFGTFFTGGASCRDGALTVVEDISVFPNPAADIITVVVADEADITSLLVLDVLGN
                     MVLQATVSADNFGMVQLDCSALAAGSYRIIAETGEQFFTAPLQVIH"
     gene            complement(14092..>15223)
                     /locus_tag="PK742_03610"
     CDS             complement(14092..>15223)
                     /locus_tag="PK742_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HQU75705.1"
                     /translation="RIALSIIRLTGTSPNRVILGFMIATGLLSMWISNTATTLVMLPI
                     AASVIDLLRNDGDGFTNGDRNFALSIMLGIAYSANIGGVATLIGTPPNMVMAGFINQQ
                     YETSISFFRWMMLGIPFAIIMTAILYVVMVKWMYPNHLGHDAEAGKLVIRQELQKLGS
                     WSPDEKRVMGVFLFAILLWTIREPMSKQFSWFTLSDTGVAMLAALLIFALPTDLKNMK
                     FVLDWSDTKTLPWGILILFGGGLALADGMSDAGLIDVIGNFVHTLNNWPLWVISGFLI
                     LVVLFMTELMSNVALVTIFLPVAAGIASGLGIPFQHLLIPITMAASCAFMLPMATPPN
                     AIVFASGHVKVRQMVRIGVVLNLISVLLLAVLAATLIPYLFG"
BASE COUNT         3881 a         3567 c         3954 g         3821 t
ORIGIN      
        1 ggtgccggct tgccgttcat tccgccctgg cttcgggaag acctggcaga atgggacctg
       61 gcagcaggga ataaactgga tgagatcatc cgcctggaag atctgcgtgg cgtggtgcac
      121 aaccacagca cctggagtga tggccggaat acgcttcggg agatggcaga agcctgcatg
      181 aaactgggct atgagtatct ggtcatcagc gaccattcca aaacagcagt gtacgccggc
      241 ggcctcgagg tggaaaaggt gatacagcaa cacaaagaga tcgaacagct gaatgttgag
      301 ctggcacctt tccgcatttt caaaagtata gagagcgaca ttctcaacga cggttcgctg
      361 gactaccccg atgacattct gcagcagttc gatctggtca tcgcatcggt gcaccagaac
      421 ctgcgcatgg atccggaaaa ggctacgagc cggctgatgg ctgccgtaga aaataggtac
      481 acggccattc tcggccatat gaccggcaga ctgttactgt cacggcccgg ttatcagccc
      541 gatcacaaaa agatcattga tgcctgcgtg gccaacggag tggccattga agtgaatgcg
      601 aatccttacc gactggatat ggactatcac tggataccct atgccatgga aaaaggattg
      661 ctcatgtgca tcaatccgga tgcacacagt gtggaaggca tcgctgatat gcactggggt
      721 gtctgtgctg cccgaaaagg cggactgctc cgtgcacaga cattgaatgc gttgccatta
      781 gaggcctttg aaaaatgggt atctgacagg cgtgctgcaa agggtattga ctaactttgc
      841 ggtctatgcg tcactacctg tcgctcatta aattctctca tactgtcttt gccctgccat
      901 tcgccctgat cgggttcttt ctggcactgc aaacgctgga cggaccgttc ccctggacag
      961 atctcttgct ggtgttggga tgtatggtct ttgcccgcag tgcagccatg gcttttaaca
     1021 gatacctgga cagtgacatt gatgcactga acgccagaac cgcggtgcgt gaaataccaa
     1081 aaggagtgat cagtcgcccg caggccctga tctttgtgat actcaacagc atcgctttca
     1141 tcgtgtgtgc cggtctgctc aatgagctgt gtttgtacct ctctccggtt gccttgctgg
     1201 tcatactggg ctattcctat accaagcgat tcacctggct ctgtcatttt gtgctgggtc
     1261 ttggcttgtc gctcgcaccg gtgggagcct ggctggcgct gaccggacag ttcgcctggc
     1321 ttccgatcgt gttcggcctg atcgtgctga cctgggtgag tggttttgat atcctgtatg
     1381 ctttgcagga tgagaccttc gaccgtgagc atcacctgca cagtgttccg gaatggctgg
     1441 gcaggagaaa ggccctgagc ttatctatca tcgttcatgt gcttacggct gcactggtca
     1501 tcgtggccgg gcagatcgca gacggaggca cctggtactg gatcggtgca ggattcttca
     1561 ccgccatgct gatctaccag cacatgctgg tcaaaccgga tgatatcagc cgggtgaatc
     1621 tggctttcgc cacaaccaac ggaatggcaa gtgtggcatt cggcatactg gtcattgcag
     1681 atatattgtt ctgatgggtc gaatccttgc catagatcat ggaaagaaac gctgcggtat
     1741 cgcggtgacc gatcctatgc gcatgatcgg ttctccgctc accacagtag ataccgaaaa
     1801 ggtgttcgaa ttcctgcatg attactttca gaaagaaaca gtggatctgt tgctggtagg
     1861 ctatccgacc aacatacagg gtgaggccac tgatgccaca ccattggtgg atcgcttcat
     1921 cgaacagtgc agaaaaaaat tcccgtcgct ggaacttcgc acctgggatg aatcgttttc
     1981 cagcaggaag gccatgcaaa cgctggtgca gagtggagtg aagaagaagg acaggaggca
     2041 gaaaggagca ctggatcagg tgagtgcagc ggtcatttta caggaatatt tgcaaaattt
     2101 gtagcatgat cttacccatt gttgcatatg gtaatccggt tttaaagaag gtggcagagc
     2161 ccatcggatc agactatccg gaactgaagg agttgttgtc caacatgtgg gagaccatgt
     2221 ataatggtcg gggagtaggg cttgctgcac cgcagatcgg tctgggcatc cgtatttttc
     2281 tggtggatag tgagcaactg gtcaaagaga atgaagaagc cgaagaagat gaagaattat
     2341 accgggtcac ccagggtatc aaaaaggcct tcatcaatcc ccggatcata gaagagaccg
     2401 gcgaaccctg gaactatgag gaaggctgcc tgagtattcc tgatgttcgg gtaaagatca
     2461 gccggaagga actggtgcgc atccggtatg tgaacgaaca gttcgaagag atcgaagaaa
     2521 cgtatgacgg catgaacgcc cgaatcatac agcatgaata tgaccatctg gaaggcatat
     2581 tgttgaccga ccacatgagt ccgctgaaaa ggagtctgct caaaggcaag cttgataata
     2641 tcagcaaggg caagatcgat gtgaactacc gcatgcgttt tctgcagcgt tagtggtttt
     2701 tgaaatcgcc acgttgccag gcctcaatga aggctttcca cgcaaacaca tcaaagatcc
     2761 ggatgggtgg attctgtccg gcataataca attctctggc gatctgcttg gtatagtaat
     2821 cctggttggc atcggcatca tagccatatg cttcgcccag ttccttgagt ttttcccttt
     2881 ccaggttctt ggctgcgatc tcatactgat cggccggaat atcggcatac aggaaagttt
     2941 cccggaaatc ttcccgcgag ggccatggaa agatgaccgt ttcagccagg aatatagtat
     3001 cgccggtcat gagctgcacc atggaatacc ggtcttcagg taatgagtca gggatgacag
     3061 cttcagcggt ttcaaaaccg atagcggaga aaatgatggt atcgccttct ctggctacga
     3121 gtgagaaaaa tcctttccag tcggtgaagg ttcccgtacc actggtcttg gaatagatat
     3181 taacaagggg aatggcccgg aggctgtcgc ttgtcatgac cagaccggag aactgaagaa
     3241 tacgtttatc ctgggcaaag agcgacagcg gaaggagaag aagtaccaga aaagagaagc
     3301 gtttcatgca actgcgaagc tacattgcat ttgtcaaatg aacgacagca cggtacttta
     3361 ctgatcagta gggaataggg ttttgctggg gcctgagatt ccagccgagt gccgattgat
     3421 acatcttaat atcagactga tctgcagata cataattgat agcagcaggg gtgacctctt
     3481 tagtagagga ataatctgct gtggagatca cataagcatc tttggccatg atacggacgg
     3541 tcacgtgcag gacgatcacg ctgatcagga gactggtgcc gatggttttt ttgatctgca
     3601 taccgctttg atgtggttaa caatatgcaa ggtatttcag tccacactgc ccgacaacta
     3661 cagattttac tgcgtatcgg gtgctgtttt caggtataaa acgccattat ttaagaatta
     3721 ttaaggattt cgtgtcattc agatgatttt ttcatgacag attcaggcgg ttatcggaac
     3781 agccccttta ttactgtcag tgcgaaaggg aaaaaaaacc cgcctcgagg agtaaggcgg
     3841 gttggcatag ctaaaagcta tgggaactga ctattgaatc tttagttgct tgacgatctt
     3901 ctcgtcacct gacatgatgc tgataaaata gacaccgggt tccacgtcac gaagttccag
     3961 gcgaatcagg ctctggtcga tcggggcact ttgaatgacc tgtccgacaa tattcagtaa
     4021 ttgcagggta cctgtgatct tttcagaaag ttctatcact acatagtctg ttgcagggtt
     4081 gggatacaga ctgtaagtaa cagtggcaag tgttctgggg cctgcacctt cactttcctg
     4141 cctgtctgta tagttggcta catgagatgg gccttcggtg gcatctgcgt taccaaatgg
     4201 cccgaataca ccactattat tcggattgaa gttattgata tccggcttga atccgcccag
     4261 attctccagt tcaggattga tcgttgttcc gcctgtgcct gtgttgcttc cctgtgcaaa
     4321 cagcgaaact gtagctattg tcagcagaaa tgtaaacagg ttcttcatcg ttcaggtgtt
     4381 cagtggttga gtactttcag ggtttgaata gttaaaacta ttcagctctt tttacaatat
     4441 atactacaaa aaaggtgctt tatcggttcg tgccggacag cattttttag cattttttaa
     4501 tataacacac tgtaaatcaa gattttaatc tatttgtgat aaatcggaaa ttggctgttt
     4561 atcggaaaaa ggacatgaaa aaggcttgca gggttcctgt aactgcatac ttttacagac
     4621 cctcttatgt accagaacaa ggccatcatc atccttcgca ccttcactcc gcaggaaatg
     4681 cgtcagatgg atgatttcgt cctgtcgccc ttcttcaaca agaaccagaa ggtagctgat
     4741 ctgtacctgg taattcgtga tagtcaccct gattttgatg cccctgtcat agagcggcaa
     4801 cgtttattcg aggccgtatt tccggggtca gcctatgatg agcagaagct gcgttacctg
     4861 ctgacagacc tgaccaagct gctggaggaa tacatctgct ggaaagcggt agaagaggag
     4921 gtgttgttca aataccattt gttattgtac agttaccggc aacggggatt ggacaagccg
     4981 ttcatgagtg tgctcaaaca agcagaaaag accctggaag actggcctcg ccgcgatgtg
     5041 tcgcatgcat tctatgagta tctgatagaa gaagataagt acaagttcag tgccttgcag
     5101 aaggaacacc tgccggaaac caacctccag aaagtagttg ataacctgga caagtatttt
     5161 attctgaaca aaatgcggta cagtgcagag atcatcaaca accggaatgt ggtggccatc
     5221 aactaccgcc tcttcctgta tgaggagatc atgaaccacc tgcgcaacaa tcctcttgac
     5281 catgtgccgg ctgccaagat ctattataat gtcatcctca cgcttaccga gccggagaac
     5341 aaactgcact attccaacct gttgttgttg ctggaagaac accggaacat tttcagccgg
     5401 gaagagcttt ttgatatgta cgtgtatgcc aagaattttt gcatcaggaa gatcaacaac
     5461 ggccacacag aatacatgaa ggaactgttc ggtctgtatc agatcatcct ggagaatcgc
     5521 atcatcttcc gtgagaatta cctgtcgcag cgggattata agaacatcat ttacctcggt
     5581 ctgcgactcg aggaattcgg ctgggtgaag tcctttatag aagagtacaa tgaccagctg
     5641 gatccccgtt acaggaagaa tgcctacacc tataatatgg cttattacca tttcttcctg
     5701 ggcgaatatg atgagaccct caccatgctt cgctctgtag agtttacgga tgtctattac
     5761 cacctcgatt ccaagtcgct gctgctcaag acctactatg aactgggtga aacggaagcg
     5821 tttttgtcgc tggtagaggc attcaagatc tatatcaagc gcaacaaact cattcctgtc
     5881 aaccaaaaga ataactacaa caacctgatc agattcgtta ccaaactgta caaatggaag
     5941 ctcagtccgc ggaataatct tcaggagatc gcctcagaga tcgaacgcac caaacccatt
     6001 gcagatgtca tctggctccg gaagaaactg gaggaagccg agcagatcgc tgcgaagcat
     6061 tcgggttcct ggcgaaaatg accggtgtat atcctgtgca aacaatgacc gaaaacattc
     6121 atttcagcaa ccggcatcgt acctttgcac cgcattcact tttaatctac acatatgtcc
     6181 cacacagacc gttttgttga ccgccatgtt ggcccttcca tggatgatat ccgcgagatg
     6241 ctggccgtga tcggagaacc ttctctggaa gcacttattg acaagaccat accacagcag
     6301 atccgtctga aaagagacct cgagctgagt gccccgctga gcgagtatga gtatctgcgt
     6361 gcgctcgatc agaaagcgtc tcagaacaag gtcttccgtt cctatatcgg actgggatat
     6421 tacaatacca tcacaccgtc ggtcatccgc cggaatatcc tggagaatcc gggatggtac
     6481 acccagtaca cgccctatca ggcagagatc gcccagggca gactggaagc actgctcaat
     6541 taccagacca tggtgagcga cctgaccgga ctggagatcg cgaatgcgtc attgctggat
     6601 gaaggaactg ctgccggtga agccatgatc atgttcttcc accataagaa caagaaagac
     6661 atcacagcac caaaattctt tgtggccgat gactgtttcc cgcaaacaat agatgtactg
     6721 cgcaccaagg ctgctcctat gggaattgaa ctggtgatcg gatctgcaga aagtttccgg
     6781 cctgccgatg actatttcgg tgcattgctg caatatccag gtgtgaacgg agatgtgcgt
     6841 gactatactt cgctgataga ggcttgtcac cagcatgaag tatgggtcac cctggcggcc
     6901 gatatcatgt cactggccat gctgaagacc ccgggtgaaa tgggtgccga ttgtgccgta
     6961 ggtaactcac agcgtttcgg tgtgccgatg ggttttggcg ggccacacgc tgcatatttc
     7021 gctaccaaag atgagttcaa gagggtcatt cccggccgta tcatcggggt gtccatagat
     7081 cgtcgcgaca agcctgcact gcgcatggca ctgcaaacaa gagaacaaca tattcgccgt
     7141 gaaaaggcga cctccaatat ttgtacagcc caggcattgc tggccatcat ggccggtatg
     7201 tatgccgcct ggcacggacc ggtgggtatt cgcaatattg caggcaacat ccaccgcaat
     7261 gcaggcaggc tgcaggaaac actcgcagcc ctgggctttg aagtggtcaa taaaaactgg
     7321 ttcgacacgc tgacgattcg ccacagcgga caggctgatg ccatccgcaa ggaagctgta
     7381 tctatgggca tcaacttcta ctatgatgct gaggtggtgc gtatttcctt agatgagaca
     7441 gttagtgagc aggatctgaa agacatcctt acagtctttg aaaaagtatc aggaaagcag
     7501 gcgaagcttt ctgatgaatc aaccggtctc aaggctgatg cattgcgaaa aacagaatac
     7561 ctgacacatc cggtgttcaa ccgctaccga agtgaaagtg agatgatgcg atatatcaaa
     7621 ggactggaga acaaagacct gtcgctcact accagcatga tcccgctcgg atcatgtacc
     7681 atgaaactca atgcagccac agagctgcta cccattacct ggccggcctt ctctgccatg
     7741 catccttttg tgcctgcaga tcaggtgaag ggctacctgg aaatcatcgc agaactggag
     7801 aaagacctgt gtgagatcac ccgttttacg tcttgttcac tccagcccaa ttccggtgca
     7861 cagggcgaat atgcaggact gatggttata cgtgcctatc accttgacag aggagatacc
     7921 cagcgaacca aagtgcttat tcctgcatct gcacatggaa cgaatcctgc ttctgctgtg
     7981 atggcgggaa tggatgtagt ggtagttgcc agcgatgcga acggttatat tgatatggat
     8041 gatctgcgtg ccaaggcaga acagcacaag aatgaactgg gtgctttgat ggtcacctat
     8101 ccttccacac atggcgtgtt tgaagagacc atcatcgagg tatgtgagat cgttcaccag
     8161 tatggcggac tcgtttatat ggacggcgcc aacatgaatg cccaggtagg tctgacctcg
     8221 ccggcgcata tcggtgcaga tgtctgtcac ctcaacctgc ataagacctt tgccattccg
     8281 cacggtggtg gtggtcccgg tatgggcccc atctgtgtga acgataaact ggctccttat
     8341 ctgcccggtc acccactggt gaaaacaggc ggcgacaaag ccatcacccc tgtaagtgca
     8401 gcaccatttg gcagcgccag tatcctgctt atttcttacg gttatatcaa gatgcttggt
     8461 ggcgaaggct gcaccatgag caccaaggcc gccattctta acgccaacta tctgaagagc
     8521 cggctggaag gcgcctatga tattctttat gtagggcaat caggccgcgt agcccacgaa
     8581 ttcatcattg acttccggaa atggaaacat accatcggac tggaagtgga agatgtggcc
     8641 aagcgactga tggactatag cttccatgca cctacggtgt ctttcccggt tgcaggtacc
     8701 ctgatggtgg agccaacaga gagcgaaagc cgtgaagaac tggatcgttt ctgcgaagcg
     8761 atgttgtcta tccgcgaaga gatacgggca ctggaagaag gtaaggctga tgcaagtgac
     8821 aacgtgctga agaatgcacc tcataccctg gccgatgtga tcgtgagcga ctggacacat
     8881 gcctacacca gagaggaagc ggtgtatcct gttccgtatc tgagggagag gaagttctgg
     8941 acctctgtga accgtatcaa taatacccac ggcgacagga atctgatctg tgtgtgtccg
     9001 ccgatggagt cgtatatgga agcgtaagga attactaagt gcggaatgcg gaatgcggag
     9061 tgatgtcctt cgcattccgc attctgcatt tctgaaaacg gtgactttca ttctaccatt
     9121 ggctcctgtg tgcatgtacc tttgtggcac aagtaattca aaaagttatg aaccccgata
     9181 ttcattttgt ttcaccgcag gaagccttat cctgcataca cagcggagat actgttttca
     9241 ttcacggcag tgcacaaacg ccggtaggca tcatgcctta tctggcagaa agaaaaaacg
     9301 agctggagca tgtacagctg gtattcatca gtctgtatgg cgatatcata ttagaccgtc
     9361 cggagtatga tgatgccttt gatctgaacg ccatgttcgt atcgggcagc gtgcgcaatg
     9421 cagtagcaga agggcgtgca gattatattc ctgtttttct cagtgagata cccctgttgt
     9481 ttcagcgtga tatcattccg ctggacgttg ccatcgttca ggtatcgcca cctgataagc
     9541 acggttactg ttctctggga gtttcagtgg atattgcccg cacagcggtc aacaaggcca
     9601 ggcatgtcat agctgtcatc aatcccaacg ttcccagaac ccacggagat agtctgatac
     9661 actccattcg ttttgacagc atggtttgga tggacgaacc gcttcaggag gtcaccttcg
     9721 gcgataaagt aggggaggaa gaactggcca tcggcaaaca tatagcagga ctgatagagg
     9781 atggaagtac gctgcaaatg ggcattggag ccattcctga tgcggtgttg cgtttcctca
     9841 ccaaccacaa gaatctgggg gcacatacag aaatgttcag cgacgggctg atcccgctgt
     9901 tcgagagtga tgtgatcaat aacaggtaca aggtcattga acccaaccgc accgtaaccg
     9961 gatttgcggt gggatcgaaa aagctgtatg actatgtgga cgataatccg gcatttgctt
    10021 tcctggatat tgactttgtg aatgatcctg cggtcatcag tcgcaacccg aaagtagtgg
    10081 ccatcaactc ctgcatagaa gtagatctta ccggacaggt ggtcagtgat tccattggca
    10141 ccaggcagta cagtggtgtg ggcggtcaga tggatttcat cagaggtgct gccatgtgtc
    10201 cggaagggaa gcccatcatt gccttatcat ccaggacgaa aaaaggacaa agccgtattg
    10261 tcaatacgct taaagcaggt gcaggtgtag tgaccaccag agcacatgtg cactatgtgg
    10321 taacggaata tggtgtggcc tatctgtttg ggaagaatct tcgccagaga gcaaaagcac
    10381 tgatcaacat ctcgcatcct gatgaccggg aagagctgca ccaagcctgt ttcgaacggt
    10441 tcaggatatt tgtgtaacaa tcagttgcag atcgtttgca accttctgat tatatcaggt
    10501 agtcttgcat gtataattta tctgcatgca aagaagaaag ttcatacgcc aggtcagttt
    10561 ggcagttccg gctctgttta catccagggc attgggtgca tggccttcgt ttctctcagc
    10621 cggggcaacg ggtcaggtat tgatcattgg tgcaggcagc gcaggtttgt atgctgcccg
    10681 ccagcttgca gatcagggaa tatcagttac cattctggaa gcatcatcac agcacggggg
    10741 cagggtcagc cctcttgatg gatttgcaga cttccatgta gaggccggcg cagaatttgt
    10801 gcatggcaaa ggcaacacca ctggagatcc tccttcattt ctctggagca gtatcaacga
    10861 ctataacagc gacctgctgc aggagtatgg agcatacaaa gaaatatatg atgtttctgg
    10921 tgttcctaag ctgtctcctc cttactggga catgcagctg gaacagtgct ggcagtttta
    10981 cctggacatg tacacctatt ccggaccgga tgtattaatg agcgactacc tcttcgatac
    11041 ctacgggata gatgaaagcc acccgtactg gcatttttac gaagcctgga ttggtgcaga
    11101 gtttggtacc tccatcaagc ggatcggaat gaacagcatt gccatcagtg agttgctctg
    11161 gaaaacaggc tccaaagatt ttctgctgga caccggatac cgtgacctgc ttgatacact
    11221 tttctttggt cctgttctgt cagatattca atacaacaag gtagtaacag gcattgatac
    11281 cggcggcgat caggtttcgg tgcaatgcga agatggcagt gtttttactg cagataaagt
    11341 gatcgtaacc gttccactca ccattctgaa gcagaacagc atcacttttg aaccaccact
    11401 tcctgcactg aaaacttctg caatagaaat catcgggatg gatgccggca tgaagatctt
    11461 tctgaagttc agcgagaact tctggggaga tgatgtgcag gatattacca ttgatggata
    11521 ctccactttt atctgggcgc ccggaatggg gaagaccggt gctactgatc acgtactggt
    11581 gtgttttgtc atgggtgaaa gggctgaata tctgagctct ctggatgccg gtgccattga
    11641 tgtggtcatg agtgaactgg attcactgtt tgacggagct gcaagtccgc tgttactgga
    11701 tcatttcatt caggattggg caactgaacc atttattggc ggagcctaca gctatccttc
    11761 gccgggaacg tatatgtctg aatcagaatc tgccaggatc gatctggcgg ccccggttga
    11821 ctgtaaatta ttctttgccg gagaagccac aagtctgcaa catcccgcca ctgtacacgg
    11881 agcactggaa agtggtgcac gggtggctga agaagtactg gcatgtccct ttcctacaga
    11941 tctgaactca ccacagtatc aggatttcca ggcctattat gaaaatggat tgatatatgt
    12001 agaatggctt ccttttttta ctgccggaga agagctgatg ctggtcaacg cacagggcca
    12061 gatggttggc aaatggccca ttgacaacag caggtcatca tctgaactag atgtgcgtca
    12121 tctgcctcgt ggtgtctata ttttatacct gatcaacgga gcaaaacgaa acagccgatt
    12181 actcattttt tcctaatttc agggatgcaa aaaattgtcc ttactgcttg cctgggtttg
    12241 tctgtattgt ctgcacaggc tcaactgaat gtagatctgc tctaccaaaa tgactatcct
    12301 gaaaatatgt ccaatatctg gggatggacc gctccgggtg gtgaggaata tgttattctg
    12361 ggaagttttg acggtacgta tctgatcgat atcacagatc cttcttcacc cgatgaattg
    12421 cagttcattg acgggaacaa cagtacctgg cgacagatag cagtatggga tcattatgct
    12481 tatgtggtga atgaatccgg tggcggcttg ctgtgtattg atctgagcgg attacccgga
    12541 actgctgcct atgcattcag tgatgctgat ggagcaggtc tgacaactgc tcaccaggtc
    12601 tgggcagatg agaatggtta tgtatatgtg ttcggttcta atctggatgg aggttcttct
    12661 gttattctga atgccaatgc tgacccgatg gacccaccaa tggtaggaag tacagatacc
    12721 tggtatgtac accatggatt tgctagagga gataccttat atgaatctaa tatataccag
    12781 ggatggttct ctgtctggga tgtctctgac aagtccatgc cggtactgtt ggccacccag
    12841 gaaactcccg gtacttttac ccataatgtg gttccttctg ataacggtca gtttctgttc
    12901 accactgatg aaaaaagcaa tggctcactg gcagcctatg atataagtga tctgagtgat
    12961 atacagtatc agtcggagtt cagggctaac cccggaacca gttccatccc gcattatgcc
    13021 tggtattttg acggatttgt ggtgaccgcc tggtacaagg acggtgtgat cataactgat
    13081 gccacgcacc cggaattcat gatcaagacc ggatattatg atacgcatcc cggaagcggc
    13141 agcggtttca gtggtgcctg ggggtgctat ccttattttc cttcaggaac actggtggta
    13201 tctgatatct ctgaaggttt ttttgtgctg catcccactt atgtacatgc ttcatttgta
    13261 gaaggaacca tcacagacag cgatagcggt ctgccggtat ttggcgcaac ggcttctttg
    13321 ctcacgctaa gtggcactac cgtaagttct tcgttggatg gcgggtatat taccggagct
    13381 gtggcagatg gtatatacac cctggaagta agtaaagcgg gatatgcaac cgcttccatt
    13441 cctgtcagtc tgagtgcagg tgtcacactt gcggaagaca ttctgctgga gcctgtaacc
    13501 gccacctgtg aatcgccggt cattctgggt gcatcttcta tcaccagctc ttctgcctat
    13561 ctggactgga ccaatgttgg cgcagatcag tacagtgtga tctacaaaaa ggttggagga
    13621 agtggcacca ttcataccac caccgcaacg aattatctgg ctaccggcct tgaaagctgc
    13681 tctacttata aatgggcggt cagagcgagt tgtccggatg gttccaaaaa gaaatcagtt
    13741 tttggcacct tctttacagg tggtgcatca tgcagggatg gtgcgctgac cgttgtggaa
    13801 gatatctctg tgttcccgaa tcctgctgca gacataataa ccgttgtggt agcagatgag
    13861 gcagacatca cttcattgct ggtattggat gttcttggaa acatggtatt gcaagcgacg
    13921 gtttctgccg ataattttgg aatggtacag ttggattgca gtgctctggc tgcgggaagc
    13981 tacaggatca ttgccgaaac cggcgaacaa ttctttactg ctccactgca ggtcattcac
    14041 tgatcatcat ttggcgatga tatgatgtgg gttagtcagt gtgcggtcac ttcatccgaa
    14101 caggtatgga ataagcgttg cggcaaggac cgccagcaat aagacactga tcagattcag
    14161 tacgacaccg atccgcacca tttgtctcac ttttacatgc ccgctggcaa agacgatcgc
    14221 atttggcgga gtggccatcg gcagcatgaa ggcacagctt gctgccatgg tgatgggaat
    14281 gagcaggtgc tggaacggaa tgcctaagcc gctcgcaata cctgcagcta caggaagaaa
    14341 aatggttaca agtgccacat tgctcatgag ttctgtcatg aacaatacca ccagtatcag
    14401 aaaaccgctg atgacccaga gtggccagtt gttaagcgta tgtacaaaat ttccgatcac
    14461 atctatcagt cctgcatctg acatgccatc ggccaatgca agcccgccac cgaacagaat
    14521 cagaatgccc cagggcagtg ttttagtgtc agaccaatcc aggacgaatt tcatgttctt
    14581 gaggtctgtt ggtaaggcga agatgagtaa ggctgccagc atcgctacac cggtatcgct
    14641 cagcgtgaac caactgaact gtttgctcat gggctcgcgg atggtccaca aaagaatggc
    14701 gaacaggaaa actcccatga cccttttttc gtctggcgac cagctaccca gtttttgcag
    14761 ttcctgtctg atgaccagct tgcctgcttc ggcatcatga ccaagatgat tcggatacat
    14821 ccacttcacc atgaccacgt agagtatggc cgtcatgata atggcgaacg gaatgcccag
    14881 catcatccat cggaagaagg agatggaagt ctcgtattgc tggttgatga aacccgccat
    14941 gaccatattg ggcggcgtgc caataagcgt tgccacacct ccgatgttgg ctgaataggc
    15001 aatgcccagc atgatgctca aggcaaagtt cctgtcgccg ttggtgaatc catcaccatc
    15061 gtttcgcaac aggtctatga ccgatgctgc aatgggcagc atgaccaggg tggtggcggt
    15121 attggagatc cacatgctta acaatcctgt ggcgatcata aagcccagga tgacacggtt
    15181 cggactggtg cctgttagcc ggatgatgga caacgcaatg cgt
//
LOCUS       DAOMAX010000039        15081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_455_length_15081_cov_3.769666, whole genome shotgun sequence.
ACCESSION   DAOMAX010000039 DAOMAX010000000
VERSION     DAOMAX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_455_length_15081_cov_3.769666"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK742_03615"
     CDS             complement(<1..307)
                     /locus_tag="PK742_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQU75706.1"
                     /translation="MRVISGNLRGRRFQPPAKIPARPTTDIAKEGLFNILSNRIDLAN
                     TSFLDLFGGTGSISYEMGSRGCCDMLLVEKDPKSAAFIRKQAEEFGLDLQVMQMDVFE
                     "
     gene            complement(304..1104)
                     /locus_tag="PK742_03620"
     CDS             complement(304..1104)
                     /locus_tag="PK742_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HQU75707.1"
                     /translation="MEVFRHTDFDPAFTAHLELYLQLRSNGFTYACTEPDNNRLMLLN
                     NLDLSGEISDPDEKRMIGELSKEDLVFLPAARRTVIWSHLPFSLFPAEIGAGDIEKQA
                     SLLLDLSGQQICQDRYDELVVAFALPVNIYSRYRDLFPGAVHRHLAGSLLSSNAEDHV
                     IHMVIQEHLVLLSVRIDGKLHLANSYRFQTTDEFLYFLLLATEQTGFDRENGSVILSG
                     QVAEDGKLFQETAKFFNHIRLETSELWPDTADHETLPQYFTPLLKMMS"
     gene            complement(1143..1787)
                     /locus_tag="PK742_03625"
     CDS             complement(1143..1787)
                     /locus_tag="PK742_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75708.1"
                     /translation="MNKSLSRILMIVLGILIIFLIYQTVTGIKGPIEFDKESKARYEE
                     VEKRLDAIRNMQFTYKEATGKYASTWDSLMMVINHDSLSIQRKILIPKSSYDANVYGP
                     NPKLADDTTKYEVVSHSMVSIRDTLTKIIPYKLEDISIIPFSDGAKFYMKSDIIEAGG
                     GRIKVPVFVVTAPNRLILKGLDEKYFNAEAGYQLGSLYETTTEFAYKRDGVEYE"
     gene            1998..3329
                     /locus_tag="PK742_03630"
     CDS             1998..3329
                     /locus_tag="PK742_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQU75709.1"
                     /translation="MQLQVTYRQILTISLPLMLTALFNNIINLIGVAFMGRVGETELA
                     ASGIAALIYITLTMIPYGLSVGFQIIIARRAGENKSELIGRVFNSNMVFMFGVGLLLY
                     AFLVWLAPGLLRPLFQSDAVFDAGLLYLRYRGPEIVFAAVAFTLIAFYTSIGNNRIIS
                     WSALIMLIINVFFHYNLVFGKLGFPEMGIAGSGMAALISSTAAMLINFIYLWFSPYRK
                     RFHLFHFRRFEWDLVYSGLKLSGPVVLQHLLSTGTWVIFFLFIEKMGSGALAASNVAK
                     EVYMVLGVTTWGIANATNSLVSNLLGQGRHEEMFPMLGKILRISIGFSMIFCIAILVF
                     PDLFTGLFTNDPEIRELARNPVRITGACLFMMATASVLFRAVTGTGATRYSLLLELIT
                     LIIYMAYVIVLIPIMQVPLTVAWTSEFLYWLVLAILSYYYLRTGNWKKASV"
     gene            complement(3339..4883)
                     /locus_tag="PK742_03635"
     CDS             complement(3339..4883)
                     /locus_tag="PK742_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75710.1"
                     /translation="MKKLTLLALFLSVLTSLQAQESELSEGILFDGEPYLAMDPQNPQ
                     HMVVAWMGYAWLNRIAIHYRVTFDGGNTWSPPAFIPHQVDGYTCADPSLAFDSEGRVF
                     VCYIDYIEDGSAGAVYVRRSDDGGLTWNDAVEVISVEEDPGEYPLDRPWMAIDRSGGP
                     NDGNIYINTKPAPWEPIPNASYFIRSTDGGASFEDWVYTDAPGWSVGDFIASPMAVPT
                     VGSDGLLHIAYPGWDPAESLIPRIMLASSDDGGNAFSYNTILEVTGGGVDDTLPKLGY
                     SIYSDPTNPDHIIIVYMADYLADVDIYLLETPNGGATWNEPVRVNADTPGNGIMQDLG
                     WGAFNQTGDFLVSWRDRRNAGMEGYQLPAEIKGRVWHAGDTWSDEFNFSETAAPHNEI
                     LNGNGNDFMGCALSEDTAFAVWGDARDGILRIWFAKKSMADSSVWLLDLNQMPPLEVY
                     PNPANDLIQVKTNIPSKAAVFDIHGSLLFTTIVGNNQPLLISQLPPGRYQIRTSSGQT
                     ATFIKE"
     gene            complement(4903..5328)
                     /locus_tag="PK742_03640"
     CDS             complement(4903..5328)
                     /locus_tag="PK742_03640"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU75711.1"
                     /translation="MAENKTQKNNGSVAEFIASAEPSARREDLQQVVDMMHKITGLEP
                     DMWGDSIIGFGSYAYRYESGRAAEWFLVGCSPRKQALTLYLMAGHERFPDLMRRLGKY
                     KTGKACLYINKLADVNRDVLHELIAAAYHYMNDKYNNKA"
     gene            complement(5331..8009)
                     /gene="cphA"
                     /locus_tag="PK742_03645"
     CDS             complement(5331..8009)
                     /gene="cphA"
                     /locus_tag="PK742_03645"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQU75712.1"
                     /translation="MKILETRVMKGPNYWSIRRHKLVVMRLDLEDLEEKPTNAIPGFR
                     EQLEKHIPSLWEHRCSEEEKGGFFKRVEEGTWMGHVVEHVALELQTLAGLDTGFGRTR
                     GTGEKGIYNVVYSYLEEKTGIYAGQVAVDFCQALVTGDEFDLGPHIQRMREIRERERL
                     GPSTGSIVEEALKRDIPYIRLNRHSLVQLGWGVNQRRIQATIASTTSNIGVEIACDKE
                     ETKNLLQQLNVPVPKGDTAYDEEDLERIIKRIGYPIVIKPLNGNHGRGSTMHIMTWDD
                     AVAALKKAKEISRWVIVEQFITGYDYRLLVINHQFVAAALRKPAAVTGDGKRTIAQLV
                     EETNQDPRRGYGHENVLTTIKLDDHTDRLLEKMGMTINSVPAAGEEVFLKSTANLSTG
                     GTATDVTEHVHPYNVFTAERISRLVGLDICGIDIMSPDISEAMTDNRGVVLEVNAAPG
                     FRMHLAPSDGIARNVAEPVVDMLFPSGSQGRIPIIAVTGTNGKTTTTRLNAHIAKTMG
                     YKVGFTTSDGIYIQNRMLEKGDCTGPLSTKFVLMDPTIDYAVLECARGGLLKAGLGFD
                     KCDIAIVTNVTADHIGLKDINSVEEMAKVKGVVPETVKKNGYAILNADDDLVFGMRKN
                     LDCNIVLFSMDENNARVKAHCDRGGVAAVLEDGWVSIIKGTWKIRIEKVVNIPLTLGG
                     KAVFMIQNILPCIAAAFVQGFKVEDVRLALNTFIPSPTMTPGRMNVFQFKHFEVLVDF
                     AHNPDGFEAISKYLEKVDAHPKVGLIAAAGDRRDEDIRELGRIAGRTFDEVVIRQDKN
                     LRGRTDIEIMNLIKEGITEVNPDMPVICINPEADSIKHVIENAVKGSHITILSDVIAE
                     ALDLILQYKEKDDEFAFHKEEIPNRHEAGAASQQSQ"
     gene            8127..8960
                     /locus_tag="PK742_03650"
     CDS             8127..8960
                     /locus_tag="PK742_03650"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQU75713.1"
                     /translation="MVPKGVLIAVGGAEERNTDSKGKLEVLRQILARARGKQSVVEVI
                     PTASGIPRQIGNEYLHAFTHLGCSEVRIMPIRTKKDTEKKDYFSRLEHADIVMLSGGN
                     QARLSRAFLETGLLQLLQQRHQNDEHFVIAGTSAGAMAQSRHMINGGAPAEALKHGKA
                     RLIEGLGFIREAIIDSHFINRGRFGRLMVAVAEHPELCGIGLGEDTGVIIRQHRYLEV
                     IGSGQVVIMDGRKLAYNSIVQTPGRMLSLEHLVFHLMSKGMGYDMAEGVFSDVRVMSD
                     R"
     gene            complement(8944..9864)
                     /locus_tag="PK742_03655"
     CDS             complement(8944..9864)
                     /locus_tag="PK742_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQU75714.1"
                     /translation="MKSILAIHGGAGTITRSSMSEEMEAAYLQGLRDALEAGRIAYLR
                     GDAAEDICAAAVVSLEENPLYNAGKGAVFTHAGDHEMDAAMMRGSDLAAGAVAGVRFV
                     RNPVLLARAVMKYEEHVLLSGTGADAFARLQGLETVSNNWFDTPHRRLQYEQALAEDI
                     IILDHHEKKFGTVGAVALDQKNNIAAATSTGGMTNKKFGRIGDSPLIGSGTYANNETC
                     AVSCTGHGEYFIRYAVAYDLSCRIAYGKQTLNDAADELIYKVLQDAGGEGGLIAINRL
                     GEVTLPFNSPGMYRGILHENGDVEVAIYRS"
     gene            9968..10645
                     /gene="msrA"
                     /locus_tag="PK742_03660"
     CDS             9968..10645
                     /gene="msrA"
                     /locus_tag="PK742_03660"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQU75715.1"
                     /translation="MKYVFGFAGTVLAAGWLLTGCAQNTQKTDDMSAAKQVSNAPEGS
                     ETATFAGGCFWCTEAIYEEVEGVISVVSGYSGGHVENPSYEQVCDGRTGHAECVQITY
                     DPAKVDFETLLDIHMHTHDPTTLNRQGNDVGTQYRSVIFYHNESQKEIAEKYIDIQNE
                     RGTFGNRIVTEIVPLEHFYPAENYHQDYLANNPEQPYCVFVVKKKVQHFEELYPDKMK
                     EGYRVKQ"
     gene            complement(10646..11647)
                     /locus_tag="PK742_03665"
     CDS             complement(10646..11647)
                     /locus_tag="PK742_03665"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HQU75716.1"
                     /translation="MNRADWISIWVELGESMRPGNEGLEAIIRQTEQANGWFTAAYQW
                     QAIDHIRNNWLQARALEDWLLPYQLPVNVPGRIGLIPAGNIPLVGMHDLLSVMMAGHT
                     ALVKPSSKDPFFLPWLRNQLQGIAPEAADRFLLVEKLAEFDAVIATGSDNSSRYFEYY
                     FGKYPHIIRRNRSSAAILDGSETEEQLIALGSDIFTYFGLGCRNVSKLYLPAGYDPSR
                     LLSLWEVFGSVRDHHKYRNNLDYNLTLLLLNNSRHLAADYLILAENEALHAPLATVYY
                     QHYQDEDELRLQLEALAGNLQCVVSVKPGNTLPGTTQQPGLSDYADHVDTLTFLQDI"
     gene            11723..12070
                     /locus_tag="PK742_03670"
     CDS             11723..12070
                     /locus_tag="PK742_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQU75717.1"
                     /translation="MAIHITEDCINCGACEPECPNNAIYEGGVEWAFADGTTVKGEVT
                     MLDGSVIEATSKHAPVAEDIYYIVPDKCTECVGFHEEPQCAAVCPVDCCLPDPEHVEN
                     EEQLLAKKAKMHL"
     gene            12133..12693
                     /locus_tag="PK742_03675"
     CDS             12133..12693
                     /locus_tag="PK742_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU75718.1"
                     /translation="MLQFQFDPFPELFTERLHLRRVLESDVAEIYRLRSDPRVLQYLD
                     RDPCPDLETARKWIAMVSGLVDNNEAIQWAVSEKDGEKLMGYIGLWRMTPEHHRGEIG
                     YALLPEYFRKGVMKEALRETIRYAFSGMKLHSIEAKISPGNEASIRLVEGCGFVREGY
                     FREDYFWNGKYHDTLVYSLLEGDFRP"
     gene            complement(12844..13833)
                     /locus_tag="PK742_03680"
     CDS             complement(12844..13833)
                     /locus_tag="PK742_03680"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQU75719.1"
                     /translation="MRLKTATLLTAYIGGFICVLTVPMPWFLMLPVWIFMGVACAGIG
                     MSVMHDANHGSYSPNPQVNKALGLTLNLLGGAVFNWKLQHNVLHHTYTNIHGLDDDID
                     TKAGMRFSPHQTLEDKHKGQFIYAFGLYSLITIYWIFAKDFIQYRQYRKNGVNADKPS
                     VYKRRLLQLILLKTSYLFVFLVVPVVFFQIAPWLVLTGFLLMQMTGGLILSVVFQLAH
                     SIEETTHPLPDELNTIHNSWAVHQMETTVNFSQNSRFITWYVGGLNYQIEHHLFPKIC
                     HIHYPKISAIVRDTAEQFNIPYLENKKLSEALRSHIRLLRSLGQRPTWESLLD"
     gene            14134..>15081
                     /locus_tag="PK742_03685"
     CDS             14134..>15081
                     /locus_tag="PK742_03685"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HQU75720.1"
                     /translation="MEKPIVAILCGGLTAERNISLESGEIVFQHLDRERFEPYKILIN
                     DDGWFEAQTGARLDLNTFSFQLKDEQIRPDRVFMAIHGSPGEDGKIQGYLDMQGISYT
                     CCDTVVSSVTFNKTFCKEILRDKVTMARGVVVKPGELSLLNEIRDTYTLPVFVKPNNN
                     GSSYGITKVQDWLKLEPALEEGFHYDHEILVEEGITGTEVTCGVYRHQGEIVVLPLCE
                     VRAGTHDFFNYTAKYTAGESQEIIPAPIPEQDAEAVRNVSSAIYSYLGCRGIVRIDYI
                     IAGGTPHFLEVNTVPGLSAASIVPQMARASGLSLTEFFTR"
BASE COUNT         3729 a         3574 c         3748 g         4030 t
ORIGIN      
        1 attcgaatac atccatctgc atcacctgaa ggtcaagccc gaattcttct gcctgcttgc
       61 gaatgaatgc agcactcttt ggatcttttt ctaccagcag catatcacag caaccgcgtg
      121 atcccatttc gtaagagatg cttccggtgc cgccaaacag atccaggaaa gaggtattgg
      181 ccaggtctat cctgttggat agaatattaa aaaggccttc tttggcaatg tctgtggttg
      241 gtctggcagg gatctttgca ggaggctgaa aacgcctgcc cctgaggtta cctgagatca
      301 ccctcatgac atcattttta ataagggggt gaaatactga ggcaaggttt catgatcggc
      361 tgtgtcaggc cagagttcac ttgtttccag tctgatatgg ttaaagaatt tggcagtttc
      421 ctgaaatagt tttccatctt ccgcaacctg accactcaag atcacgctgc cgttttcacg
      481 gtcaaaacct gtttgttcag ttgcaaggag cagaaagtac agaaactcat cggtagtttg
      541 aaaccggtag gagttggcca gatgcagttt tccgtctatc cgtacagaga gaagaacaag
      601 atgttcctgt atgaccatgt ggatgacatg atcttctgcg tttgatgaca gcaaactgcc
      661 cgccagatgc cggtgcactg cccccggaaa aagatcccgg tagcggctat agatatttac
      721 cggaagggca aatgcaacaa ccagttcgtc atatcggtcc tggcatatct gctgaccgga
      781 aagatccagc agcaacgaag cttgtttttc gatatcacct gcaccgatct cagcagggaa
      841 gaggctgaaa ggaaggtggc tccagataac ggtgcgtctg gctgcaggca ggaacacaag
      901 gtcttctttt gacagctctc cgatcattct tttttcatcc ggatctgaga tctcaccact
      961 cagatccagg ttgttaagca gcatcagcct gttattgtca ggctccgtac aggcataggt
     1021 aaaaccattt gaccgcaact gcaggtataa ctccaggtgg gcggtaaatg caggatcgaa
     1081 atcggtatga cggaacactt ccatgtccgg caaagttcgt aaaacaggag cgttttaccg
     1141 gcttattcgt actctacacc gtctcgttta taagcaaact ctgtggtggt ctcatagaga
     1201 cttcccagct ggtagccggc ttcggcgttg aaatactttt catcaagtcc tttcaggatc
     1261 agtctgttgg gggcggtaac cacaaataca ggcactttga tccttccgcc acctgcttca
     1321 atgatatcag acttcatata gaattttgct ccgtcactga agggaatgat gctgatgtct
     1381 tccagtttat agggaatgat cttggtgagg gtgtctctga tggaaaccat agagtggctt
     1441 accacttcat atttggtggt gtcatctgcc agcttaggat tggggccgta aacattagca
     1501 tcatagctgc tcttaggaat cagtatttta cgctggatgg ataagctgtc atggttgatg
     1561 accatcatca gactatccca ggtagaagca tattttcctg tagcctcctt ataggtgaac
     1621 tgcatattgc gaatggcgtc aaggcgcttt tccacctctt cgtagcgagc cttgctttcc
     1681 ttgtcaaatt caatagggcc tttgatacct gttactgtct ggtagatcag aaatatgatc
     1741 aggattccca gcacgatcat cagaattctg gagagcgatt tgttcataca ggagtttgat
     1801 tatagcaccg caataatact atttttccac gataacaatc ttcacagacc attctttact
     1861 tcgagttaac acaaggggta acaagcaggt aaggtttgtg cttatcttca cgtctgatgg
     1921 ctcctttcat tgtgcaggca ggttccggaa acaggaattt gacaaaacgg tgaaacgctg
     1981 tccagccaac cagaactatg cagcttcagg taacctatcg ccagatcttg acgatctctc
     2041 tgccgttgat gctgaccgca ttgttcaata acatcatcaa cctgatcggg gtggctttta
     2101 tgggacgtgt aggcgaaacc gagctggcag ccagtggcat agctgccctg atctacatta
     2161 ccctgaccat gattccctat ggcctgtcgg tcggttttca gatcatcata gccagaaggg
     2221 caggtgagaa taagtcagaa ctgataggca gggttttcaa cagcaacatg gtattcatgt
     2281 ttggtgttgg tcttttgctt tatgcttttc tggtttggct ggcacccggg ttgctgagac
     2341 ctttgttcca gtctgatgct gtatttgatg cggggctgtt gtatctgcgg taccggggtc
     2401 cggagatcgt atttgcagcg gtggctttta ccctgatcgc attttacact tctattggca
     2461 acaaccgcat catttcctgg agtgctctga tcatgctgat catcaatgtc ttctttcact
     2521 acaacctggt attcggcaaa ctggggtttc ctgaaatggg catagcaggt tccgggatgg
     2581 cagcgctcat aagcagtacg gctgccatgc tgatcaactt tatctatctg tggttctcgc
     2641 catacagaaa acggtttcat ctgtttcact tcaggcggtt tgaatgggat cttgtgtact
     2701 ctggtctaaa actatcaggg ccagtggtct tgcagcattt gctgagtaca ggtacctggg
     2761 tcatcttttt tctgttcatt gaaaaaatgg gcagcggtgc gctggcagcg tccaatgtgg
     2821 ccaaagaagt ttacatggtg ctgggggtga ccacctgggg aatagccaat gccaccaaca
     2881 gcctggtgag taatttgctg ggtcagggca ggcatgaaga gatgtttccg atgttgggga
     2941 agatcctgcg catcagcatc ggtttttcga tgatcttttg catagctatt ctggtgtttc
     3001 ctgacctgtt caccggactg tttaccaatg atccggagat ccgggaactg gccagaaatc
     3061 cggtgcgtat aaccggagcc tgtctgttca tgatggccac tgccagtgtt ttgttccgtg
     3121 ctgttacagg cacgggggct acccgttatt cgttgttgct ggaactgatc accctgatca
     3181 tttatatggc ctatgtgatc gtattgattc ccatcatgca ggttccacta actgttgcct
     3241 ggaccagtga atttctttac tggctggtgc ttgctatatt gagttactat tatctgcgaa
     3301 caggtaactg gaagaaggca agtgtgtaaa cgccggtttt attccttaat gaaagttgct
     3361 gtctgaccag agcttgttct gatctggtat cttccaggag gcaactgcga aattaaaagc
     3421 ggttggttgt tacccacaat ggtcgtgaaa agcaatgatc cgtgtatgtc aaacacagca
     3481 gcttttgaag gtatatttgt ttttacctga atcaggtcat tggcaggatt cgggtacacc
     3541 tccagaggcg gcatctgatt gagatccagc agccatacgc tactgtctgc catgctcttt
     3601 ttggcaaacc atattcgcag tatgccatca cgggcatctc cccatacggc gaatgcagtg
     3661 tcttctgaca aggcacaacc catgaagtcg ttgccatttc cattcaatat ttcattgtgc
     3721 ggagctgcag tttcactaaa attgaattca tcactccatg tgtcgcctgc atgccaaact
     3781 cttcctttga tctcagccgg gagctggtat ccttccattc ctgcattgcg gcgatcacgc
     3841 cagctaacca gaaaatcgcc ggtctgattg aacgctcccc aacccaggtc ttgcatgatc
     3901 ccgtttccgg gggtatctgc attcaccctg acaggttcat tccaggttgc tccgccgttc
     3961 ggtgtttcga gcaggtagat gtctacatcc gccagatagt ctgccatata tacgatgatg
     4021 atatggtcag gatttgtcgg gtctgagtat atgctgtagc ccaatttggg aagcgtgtca
     4081 tccacaccac ctccggttac ctctaaaatg gtgttataac tgaaagcatt tcccccatca
     4141 tcactgctgg ccagcatgat ccgggggatg aggctttcag caggatccca tccgggatag
     4201 gcaatatgca gaaggccatc agaacccacg gttggtacag ccatcggcga tgcgataaaa
     4261 tcaccgacac tccagccggg ggcatcggta tatacccagt cttcaaagga ggctccgcca
     4321 tcggttgacc ggatgaagta ggaggcattg gggatgggtt cccagggagc aggcttggtg
     4381 ttgatataga tgttgccatc attcggacca ccggaacggt cgatggccat ccagggccgg
     4441 tcaaggggat attcacccgg gtcttcctcc acactgatga cctcgacggc atcattccag
     4501 gtaagccctc cgtcgtcaga ccgtcgcaca tataccgcac ccgcagaacc gtcttcgatg
     4561 taatcaatat agcaaacgaa caccctgccc tcgctgtcga atgccagaga aggatcagcg
     4621 caggtataac catctacctg atgcggaatg aaagctggag gactccaggt gttaccacca
     4681 tcaaaggtca ccctgtagtg tatggcaatc ctgttcagcc aggcgtatcc catccatgcc
     4741 accaccatat gttgcgggtt ctgcggatcc atcgccagat atggttctcc atcgaacagg
     4801 attccttcag acaactcaga ttcctgggct tgcaatgatg ttaatacaga caggaacagc
     4861 gccaggaggg taagtttttt catgtgtgga atgcggcaac aatcatgcct tgttattgta
     4921 tttgtcgttc atgtagtggt aggcagcagc aatgagttcg tgcagcacat cacggttcac
     4981 atcagccagt ttgttgatgt acagacaggc tttgccagtc ttatatttcc ccagtctgcg
     5041 catgaggtca gggaagcgtt catggccggc catcagatac aaggtaaggg cctgtttgcg
     5101 gggagaacaa ccaaccagaa accattcggc agcccgacca ctttcatacc gataggcata
     5161 tgatccgaaa ccgatgatgc tatcgcccca catatcaggt tccagtccgg tgatcttatg
     5221 catcatatca accacctgct gtaggtcttc tcttcttgcc gatggttctg cagatgctat
     5281 aaattcagcc acagagccat tgttcttttg tgtcttgttc tcagccatga tcactggctt
     5341 tgctgagaag cagcaccggc ttcatgtctg tttgggatct cctctttatg gaaagcgaat
     5401 tcatcatctt tttctttata ctgcaggatg aggtcaagtg cttcggctat cacatcgctc
     5461 aaaatagtta tatgacttcc ttttactgca ttttcgatca catgtttgat ggaatcagct
     5521 tccgggttga tgcagatcac cggcatgtcc gggttcactt ctgtgatacc ttctttgatg
     5581 agattcatga tctcgatatc ggttcttccc cggaggttct tgtcttgtct gataacaacc
     5641 tcatcaaacg tgcgacctgc aatacgcccc agctcccgga tgtcttcatc ccggcggtcg
     5701 ccggcagcag cgatcagtcc tacttttgga tgggcatcta ctttttccag gtatttgctg
     5761 atggcttcaa acccatccgg gttatgagca aagtcaacca gcacttcaaa gtgtttgaac
     5821 tggaacacat tcattcttcc cggtgtcatg gtaggtgagg ggataaaggt gttgagtgcc
     5881 agcctgacat cttccacttt gaagccctgc acgaatgcgg cagcgataca gggcaggatg
     5941 ttctggatca tgaacactgc tttaccaccg agagtaagcg gaatattcac cactttttca
     6001 atacgaatct tccaggttcc tttgataatg ctcacccatc cgtcttccag tacggcagcc
     6061 acgccgcccc ggtcgcaatg tgcttttaca cgagcattgt tttcatccat gctgaacaag
     6121 acgatgttgc agtccaggtt cttacgcata ccgaatacca ggtcatcatc ggcattcaaa
     6181 atggcatatc cgtttttctt caccgtttcg ggtacaactc ctttcacctt cgccatctct
     6241 tccacagagt tgatgtcttt gaggccgata tgatcggcgg tcacattggt gacaatagcg
     6301 atatcacatt tatcgaatcc caatccggct tttagcagtc cgcctctggc gcattccagc
     6361 acggcataat cgatggtggg atccatcagg acgaatttcg tgctgagtgg accggtacaa
     6421 tcaccctttt ccagcatacg attctggata tatatgccat cggaggtagt aaaaccaacc
     6481 ttgtagccca tcgtcttggc aatatgcgca ttgagcctgg tggtggtggt ctttccgttg
     6541 gttcctgtta ctgcaatgat cgggatcctt ccctgactgc cgcttggaaa cagcatgtcc
     6601 acaaccggtt cagccacatt tctggcaatg ccatcggaag gtgccaggtg catacggaat
     6661 cccggtgcag cattcacttc gagaacgaca ccgcggttat cagtcatggc ttcagagata
     6721 tcggggctca tgatatcgat cccgcaaatg tccaggccaa ccaggcgaga aatgcgttca
     6781 gcagtgaaga cattatacgg atgcacatgc tctgttacat cggttgcagt tccgccggtt
     6841 gaaagattgg ctgttgattt caggaagacc tcttcgccgg ctgcaggaac tgagtttatg
     6901 gtcatgccca ttttctccag tagcctgtca gtatggtcat ccagtttgat ggtggtgagc
     6961 acattctcat gtccgtaacc tcgtcgcgga tcctggttgg tttcttccac cagctgagcg
     7021 atggttcgtt tgccatcacc ggttactgct gcaggcttac gcaaagcagc ggccacgaac
     7081 tggtggttga tgaccagcag acggtaatca tatccggtga tgaattgctc cacaatgacc
     7141 catcgggaaa tttccttggc tttcttgaga gcggctacgg catcatccca ggtcatgata
     7201 tgcatagtag agccgcggcc atgattgcca ttcagtggct tgatgacgat ggggtatccg
     7261 atgcgtttga tgatgcgttc gaggtcttct tcatcatagg cagtatcacc ttttggaacc
     7321 ggcacgttaa gctgttgcag caggttcttg gtttcctctt tatcacaggc tatctctaca
     7381 ccaatattcg aggtagtgga ggcaattgta gcctgtattc tcctttggtt gacgccccag
     7441 ccaagctgca ccagagagtg gcgattaagg cgaatgtatg gaatatcacg cttgagtgct
     7501 tcttccacaa tgctgcctgt ggaggggccc agtctttctc tttcgcggat ctcccgcatg
     7561 cgctggatat gcggacccag gtcaaactca tcaccagtca ccagcgcctg acagaaatca
     7621 actgccacct gcccggcata gatgccggtc ttctcttcca ggtaggaata gaccacatta
     7681 tagatccctt tttcgccggt tccgcgggtg cggccaaatc cggtatccag gccagccagg
     7741 gtttgtaatt ccagtgccac atgctctacc acatggccca tccaggtacc ttcttccacc
     7801 cgcttgaaga atccgccttt ttcttcttcg ctgcaacggt gttcccagag actgggaatg
     7861 tgcttttcca gctgttcgcg gaaacccgga atagcattgg tgggcttttc ttccaggtct
     7921 tcaagatcca gtcgcatgac caccagtttg tgtctgcgta tgctccagta gttcgggcct
     7981 ttcatgaccc tggtttccag tattttcata tacgttaagt gactgccaaa atacggaaat
     8041 cctgtacaag gttttatttc caccatcggt gtggaaatgt gcaggccgaa ttgtgggggg
     8101 cttctaactt tgctgcaaac ttgtgtatgg taccaaaagg tgtgctgata gcggttgggg
     8161 gggcagaaga acgcaataca gacagcaagg ggaagctgga ggtgctccgc cagatattgg
     8221 cgagggccag aggaaagcaa tctgtggtag aggtgatacc caccgcctca ggcataccca
     8281 ggcagatcgg aaatgagtac ctgcatgcct ttacccatct gggttgcagt gaagtgcgaa
     8341 tcatgcccat tcgcaccaaa aaggatacag aaaaaaaaga ctatttcagc aggctggaac
     8401 atgctgatat cgttatgttg tctggcggta atcaggccag gctcagcagg gcctttctgg
     8461 aaaccgggtt gctgcagcta ttacagcagc gtcatcagaa cgatgaacat tttgttattg
     8521 ccggaaccag tgcgggggcc atggcacaat caagacatat gatcaatggg ggcgctcctg
     8581 ccgaagcatt gaagcacggc aaggccaggc ttattgaagg gctgggcttt atcagggagg
     8641 cgatcattga ttctcatttt atcaaccgcg ggcgatttgg ccggttgatg gtagccgtag
     8701 ctgaacaccc tgaactgtgt gggatcgggc ttggcgagga tacgggagtc atcatcaggc
     8761 agcatcggta tctggaagtg ataggctccg gtcaggtggt gataatggat ggccgaaagc
     8821 tggcatacaa cagcattgta cagactccgg gaaggatgct cagcctggag cacctcgtct
     8881 ttcacctgat gtcaaaagga atgggatacg atatggctga aggagtcttt tcagatgtcc
     8941 gggtcatgag cgatagatag ccacttccac atcgccgttc tcatgcagaa tacctctgta
     9001 cattcctgga gagttgaatg gcaatgttac ttcacccagc cggttaatgg caatgaggcc
     9061 gccttcgcca ccggcatcct gcagtacctt gtatataagc tcatctgcag catcatttaa
     9121 agtttgtttt ccatatgcga tcctgcagga aaggtcatag gcaacggcat accggatgaa
     9181 gtactctcca tgaccggtgc agctgacagc acaggtttca ttgttggcat aagtgccgga
     9241 accaatcaga gggctatcgc caatgcgacc aaactttttg ttggtcattc cgcctgtaga
     9301 ggtagcggca gcaatgttgt ttttctgatc aagggcgact gctccaacgg taccgaattt
     9361 cttttcatga tggtccagta tgatgatatc ttcggccagt gcctgttcat attgtagccg
     9421 gcgatgtggt gtgtcaaacc aattgttgga aacagtttca aggccctgaa ggcgtgcaaa
     9481 tgcatctgca cctgttccac ttaacagcac atgttcttca tatttcatga ccgcacgagc
     9541 caggagaacc gggttgcgca caaaccgcac accggcaacg gcccccgcgg caagatctga
     9601 accacgcatc atggctgcat ccatctcatg atcacctgca tgtgtgaata cagcgccctt
     9661 cccggcatta tacagcggat tttcttccag agagaccact gcggcagcac agatatcttc
     9721 tgcagcatca ccccgcaaat aagctattct gcctgcttcc agggcatcac gaagtccctg
     9781 caggtaggct gcttccattt cttcagacat agatgatctg gtaatggtgc ctgctccgcc
     9841 atgtatggcc agtatgcttt tcatcctgca aagatggtgc aattgcgttt acaaaacctt
     9901 tactttgccc aaggcatgtg gctgaacttt caaagcatac ctgtcgttct cctttcaaac
     9961 aattgtgatg aagtatgtat ttggatttgc cggtacggta ttggcagcag gttggctgct
    10021 gaccggctgt gcacagaaca ctcaaaaaac cgatgatatg tcagcagcaa aacaagtatc
    10081 caatgctccg gaaggcagcg aaacagccac ctttgcagga ggttgtttct ggtgtacaga
    10141 agctatttat gaagaggtgg aaggtgttat cagtgtggtg agcggatata gcggcggaca
    10201 tgtggagaac cccagctatg agcaggtttg cgatggtcgc accggtcatg ccgaatgtgt
    10261 gcagatcacc tatgatccgg cgaaggtgga ttttgaaacc cttttggaca tacatatgca
    10321 tacacatgat cctaccaccc tgaaccgcca gggcaatgat gtaggaacgc agtatcgcag
    10381 tgtcatcttt tatcacaacg aatcacaaaa agagatcgcc gagaagtata tcgacatcca
    10441 aaatgaacga ggtacttttg gcaataggat cgtgacagag atcgttccgt tggagcattt
    10501 ctaccccgct gagaattatc accaggatta cctggccaac aatccggagc agccgtattg
    10561 tgtgtttgta gtaaaaaaga aggtacagca ttttgaagag ctgtatccag acaagatgaa
    10621 agaaggctac cgggtaaaac agtgatcaga tatcctgtag aaaagtcagt gtgtccacat
    10681 gatctgcata atcagacagc ccaggttgct gtgtggttcc cggtaaagta tttccgggtt
    10741 ttacagacac cacacattgc aggtttcctg ccagagcttc cagttgaagc cggagttcgt
    10801 cttcatcctg ataatgctga taataaaccg tggcaagcgg ggcatgtaaa gcttcatttt
    10861 ctgccaggat aaggtaatcg gccgccagat gcctgctgtt attgagcaac agtaacgtaa
    10921 ggttgtaatc caggttattc ctgtatttat ggtgatcacg aacggaacca aaaacttccc
    10981 acaaagaaag tagccgggag ggatcatatc cggctggcag atataactta cttacatttc
    11041 tgcaacccag gccgaagtag gtgaatatgt cacttccaag agcgatcagt tgttcttctg
    11101 tttcgctgcc atcaagtatg gcagcagaac ttctgttgcg tctgatgatg tgcgggtatt
    11161 tcccgaaata gtattcaaag tacctgctgc tgttatcgct gccagttgca atcaccgcat
    11221 caaattctgc caatttttct accagcagaa agcggtctgc tgcttcagga gcaattcctt
    11281 gcagctgatt gcggagccag ggcagaaaga atgggtcttt gctactcggc tttaccaaag
    11341 ctgtatgacc tgccatcatt actgaaagca gatcgtgcat accaaccaat gggatatttc
    11401 cggccggaat caaaccgatc cgcccgggta cattgaccgg aagctgatag ggtagcagcc
    11461 agtcttccag cgccctggcc tgtagccagt tgtttcgtat gtggtcaatg gcctgccact
    11521 ggtaggcagc agtaaaccac ccgttggcct gttctgtctg acggatgatg gcttccagtc
    11581 cctcgtttcc ggggcgcata ctctcgccca gttcaaccca aatacttatc cagtcagctc
    11641 tgttcataaa tcctcgtcgc aaagtgtttg acaattagat ggtcagcctc tatatttgga
    11701 accgatcaac gcttgtaaag ttatggcaat tcatattaca gaagactgta tcaactgcgg
    11761 agcctgtgag cccgagtgtc ccaacaatgc gatctacgaa ggcggggtag agtgggcttt
    11821 tgccgatggc accaccgtga aaggagaagt tacaatgctg gacggcagcg ttattgaggc
    11881 cacttcaaaa catgctccgg tggctgaaga catttattat attgtacctg acaagtgcac
    11941 tgaatgtgtg ggttttcatg aagagccaca atgcgctgct gtatgtccgg ttgactgctg
    12001 cctgccggat cctgagcatg tggagaatga agaacagctg cttgcaaaaa aagctaaaat
    12061 gcacctttga caatcatcac tatatgatga accccgccct gtgcggggtt tttttatctt
    12121 catagctgac atatgctgca atttcaattc gatccttttc cagagctatt cacagagagg
    12181 cttcacctga gaagggtgct ggagtcagat gtagcagaga tctatcgttt gcgttctgac
    12241 ccgagagtgc ttcagtatct ggaccgtgat ccatgtcctg atctggagac cgctcgtaaa
    12301 tggatcgcaa tggttagcgg gttggtagat aacaatgaag ctatacagtg ggcggtgtca
    12361 gaaaaagacg gcgagaaact catgggttat atcggattgt ggcgaatgac acccgaacac
    12421 cacaggggag agataggcta tgccctgctg cctgaatatt tccggaaagg tgtcatgaaa
    12481 gaagctttga gggaaaccat cagatacgca ttcagtggta tgaaactgca ctctattgaa
    12541 gcgaagatca gtcccggaaa tgaggccagt atcaggctgg tggagggatg cggatttgtc
    12601 cgcgaagggt atttccgcga agattatttc tggaacggca aataccatga cacccttgtt
    12661 tatagtttac tggaaggtga tttccgtcca tgatagatct gactgaagtt ctggttccgg
    12721 aagacaaaca cgttctattg cgccccgctt ccatgcaaga cctgtctgct tggaagaatt
    12781 ctcactgtga ctgatgtaat atgaataaag cttatatggg gctgatcaag tcaaattgac
    12841 cagtcagtcc agcaagcttt cccatgtagg cctttgtcca agcgacctta aaagccttat
    12901 atgagaacgg agggcttcag aaagcttttt attctccagg taaggaatat taaactgttc
    12961 tgctgtatct ctgactatcg cagatatttt agggtaatgg atgtggcaga tctttggaaa
    13021 caggtggtgt tcgatctggt aatttaaacc acccacatac catgtaatga acctggaatt
    13081 ttgagagaaa ttaactgtgg tctccatttg atgcacagcc cagctattat gaatggtgtt
    13141 caactcatct ggcagggggt gtgtggtttc ttcaatgctg tgcgcaagct gaaatacgac
    13201 tgagagtatc aggcctcctg tcatttgcat caaaaggaat cctgtcaata ccagccaggg
    13261 agcgatctgg aagaacacta ctggtacaac aaggaaaaca aataagtagg atgtcttgag
    13321 caatatcaat tgcagcaggc gtcttttgta aacggatggt ttgtctgcat tgacaccgtt
    13381 ctttctgtat tgccggtatt gaatgaagtc tttggcaaag atccagtata tggtgatcag
    13441 cgaatacagt ccaaaagcat agataaactg tcctttgtgt ttgtcttcca gtgtttgatg
    13501 aggagagaat ctcataccgg cttttgtatc tatgtcgtca tccaaaccat gaatattcgt
    13561 gtaggtatgg tgcagcacat tatgttgcaa tttccagttg aatacagctc caccaaggag
    13621 attcagggtt agccccaggg ccttatttac ctgagggttt ggtgaatatg agccgtggtt
    13681 ggcatcatgc atgacactca tgcctatacc agcacaagct acccccatga aaatccatac
    13741 agggagcatc agaaaccagg gcataggcac ggtcagaaca caaataaatc caccaatata
    13801 agctgtcaac aaggtggctg ttttgagcct catagctgtg ttggcaaagg tgtctatgcc
    13861 ttcttgctga aaatagtgtt gaacatttga ccgtaagacg tggtaaaact cgttaccgtc
    13921 ctggccaata aacttgaccg cacctttctt ttgtgtcaag ggaaatgata ctgataagta
    13981 ctaaaggtac gataacttta ttcaggtgcc ggtgataatt caatccattc atcggccagc
    14041 agaatatccg ccaaaagcat tatgcaccgc atgacgttgg caaaacaact gtagttgccc
    14101 ggcgttgatg atttattttg tgaaaacatc tgtatggaaa aacccattgt ggcgattctg
    14161 tgcggcggat tgaccgccga acgcaatatc tcactggaaa gcggtgagat cgtattccag
    14221 catctggaca gagaaaggtt tgaaccatat aagatcctga tcaatgatga cggctggttt
    14281 gaagcccaaa caggagcaag gctcgacctg aatacctttt cctttcagct caaggatgag
    14341 cagatcagac ctgatcgggt attcatggct attcatggtt ctcccggcga agacggaaag
    14401 atccagggct acctggatat gcagggcatc tcctatactt gttgtgatac agtggtttcg
    14461 tcagtcactt tcaataagac cttttgtaaa gaaatactca gagataaggt aaccatggca
    14521 aggggtgttg ttgtaaaacc gggtgaatta tctctgttga atgagatcag agatacctat
    14581 acacttcctg tatttgttaa gcctaataac aacggatcga gttatggtat taccaaagtg
    14641 caggactggt tgaaactgga accggcactg gaagaaggat ttcattacga ccatgagatc
    14701 ctggtggaag aaggaataac cggaaccgag gtgacctgtg gggtgtaccg acatcaggga
    14761 gaaattgttg tactgccact ttgcgaggtg cgtgccggaa cacatgattt tttcaattat
    14821 accgccaaat acactgccgg agaaagccag gagatcattc ctgcacccat acctgaacag
    14881 gatgcagaag cggtaaggaa tgtttcatct gccatttaca gctacctggg ttgcagaggt
    14941 attgtcagaa tagattatat cattgctggt ggtacaccac actttttaga ggttaatacc
    15001 gttccgggtc tgagcgcagc cagcatagtg ccgcagatgg ccagggcctc cggacttagc
    15061 ctgacggaat tcttcaccag g
//
LOCUS       DAOMAX010000040        15079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_456_length_15079_cov_4.288538, whole genome shotgun sequence.
ACCESSION   DAOMAX010000040 DAOMAX010000000
VERSION     DAOMAX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15079
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_456_length_15079_cov_4.288538"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="PK742_03690"
     CDS             complement(<1..1898)
                     /locus_tag="PK742_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75721.1"
                     /translation="MRRYILIFVLFMPIFSMAQTAIHGVINDYTIATGIGCDNKLEVT
                     DAAAFSPGDKVLIVQMKGAVIDSSNTSSFGTILNWGTAGKYELNELADVGDDHLQLAY
                     LTENTYDFSFRVQVVRVPVYDDVLVDSVLTCPAWDGETGGILALIVNGTLTLEADMDV
                     SEKGYRGGEFTNFPDSCPFGFSWNGYYTTTTSGNGAIKGEGFTSVSDARRAGRGKLAN
                     GGGGGNDHNAGGGGGTLGGEGGEGGERDVDLFSCPGPGTGRPGVLPDHSNAENRIWLG
                     GGGGAGHGNNGFAKAGGHGGGIIFLQVNSLDGNGFSIRSNGGSPASANGDGGGGGGAG
                     GSLLLDVSTLSSDVLVELKGGNGSNITGNACTGPGGGGGGGLVRHTGASLPAGFLTDY
                     GGGAAGITTTASSPCFNETNGATDGATGQTIADWPLLEADIPYVPLMATISNDTTICA
                     GNIVELTAGGGTTFSWSPPDYLSATDIAEPTCFPDASVTYTVIVGNGTACFDTVSVSV
                     EVEPGLTVVAGPDTTLCGANQIQFFASGGDTYTWSPGTGVSNVNIADPIVFVSTTTDY
                     VVTASNGTCTGTDTVSVIIYANPEVVTLNDTTICLGESLDIFADGALYYNWFPETAVP
                     CSDCSSMS"
     gene            2048..2482
                     /locus_tag="PK742_03695"
     CDS             2048..2482
                     /locus_tag="PK742_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75722.1"
                     /translation="MNQSNRSVPGWYWILAVLFLLWNLMGVSQFFAITFISDESLARL
                     SVEERALYESYPGWVTVVFALAVFGGSIGSIGLLLRKRWARLAFIISLIGIVPQMIQN
                     VFMTDAREVYGPGSVVMPVMILVLGIFLVWYAGMCIRKGWLR"
     gene            2732..2983
                     /locus_tag="PK742_03700"
     CDS             2732..2983
                     /locus_tag="PK742_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75723.1"
                     /translation="MSFGSIAEMIATLKRNARPKREGYKPDQKKHLFRREADPAKLEE
                     ARQERESHRRNAGRLRIIIFLFLIISVLALYLFSSRVFV"
     gene            3078..4268
                     /gene="kbl"
                     /locus_tag="PK742_03705"
     CDS             3078..4268
                     /gene="kbl"
                     /locus_tag="PK742_03705"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQU75724.1"
                     /translation="MYGSIQQRLQHELQEIKDSGLYKNERIIASPQGADITLADGSQV
                     INFCANNYLGLSAHPKVIEAAKKAIDTHGYGMSSVRFICGTQDIHKELEEKIATFLGT
                     EDTILYAAAFDANGGVFEPLFNEEDAIISDALNHASIIDGVRLCKAQRFRYEHNNMDD
                     LEAKLKEASGARTRIIVTDGAFSMDGTIARLDLICDLAEKYDALVMIDECHASGFLGK
                     TGRGTHEHRGVMGRIDIITGTLGKALGGASGGFTSGRKEIIDMLRQRSRPYLFSNTLA
                     PSITGASIAVLDLLSETTELRDKLESNTKYFREKMTAAGFDIKPGEHPIVPIMLYDAV
                     LSQQMAEKLLAEGIYVIGFYYPVVPKGQARIRVQLSAAHDRHHLDKAIEAFIKVGKEL
                     GVVS"
     gene            4327..5592
                     /locus_tag="PK742_03710"
     CDS             4327..5592
                     /locus_tag="PK742_03710"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75725.1"
                     /translation="MRLYVTGLLFSVLLCTGQLFAQQTRAALFLGNSYTYVNDLPKMI
                     ADVALTTGDTLIYDSNTPGGYTLQQHSTNATTLAMISTGAWDFVVLQEQSQLPSFPIE
                     QVEVEVFPYAALLDSLISQANPCTETVFYMTWGRKNGDAMNCPFWPPVCTYTGMDDLL
                     RERYEIMAADNQALVSPAGAVWRYIRETDPTIELYSADESHPSVAGTFATACAFYSVM
                     FRKDPVLTSYDAGLDPVLASTIRAAASAVVFDSLDHWHVGEYDPVAAFSVWPGPEGHI
                     ETENLSEQADTWQWSMGDGTVYTDAEPAHTYAESGSYMVQLIASRGDCPGDTSEQTIL
                     VTVSEPNVVTNMIPGLSWVLSGNVLTLTTSTEAIRYRMLDSSGKVLTSGTIPAAGHLN
                     LPLDEFAAGFCLLQLRSGRGQQIIPVPVH"
     gene            5669..6103
                     /locus_tag="PK742_03715"
     CDS             5669..6103
                     /locus_tag="PK742_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQU75726.1"
                     /translation="MAGKKKTDPAALLLLMDQAVRHAKQQLVAALQRSGLELTADQWT
                     LLRIVVESDGIAQHVLADQSGKDRPTVTRMLRLMADRGWVVQEPAPGDRRSFLILPTK
                     AGQQVWKAGQAVVKEQLRQTFPKSTGTLQKSTRKLCRELLKK"
     gene            6227..7744
                     /locus_tag="PK742_03720"
     CDS             6227..7744
                     /locus_tag="PK742_03720"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQU75727.1"
                     /translation="MQKLLTAFTVFLVAITIKAQTEGDQLFATDQVVTIELTFTQEGF
                     WDSLVANYETASYMLADLDITDQTGTYSFPQIGVRLKGNSSYGHPGNKKSFKIDFNEY
                     VSGQNYDGLKKLNFSNNFKDPTFLREKIYSDLCLSNDLPAPRTSYADVYMNGTHWGFY
                     TVIEQIDDQFLDWNMEDDGGNMFKAGDAFDGPGGEADLVYYGADQSSYTERYEIENNE
                     DLNDWTDLIAFIDFINNTTDAEFETGIDDRLETDQYLRSMAMDNLFSNLDAYINSARN
                     YYIYHHEGTGRWNWIKWDCNEAFGSYQGGPMGGGLSMTELDLHYVNNSRPLLERIMEI
                     DGMQARFDDALCYWINGDFSSAKIQEEIDRYYELIKPYVYADENKMYTDIQFENNIYE
                     DITTGGGPGGGTIFGLMSFMEARLDFVEGALDCTAVVAIDESSAHDAFFCRPNPAGQV
                     VWVQTIEPERITVFDLKGQQVLQAASGWVDISALPEGLYILRSDSGNTTRLSVVR"
     gene            7855..8181
                     /locus_tag="PK742_03725"
     CDS             7855..8181
                     /locus_tag="PK742_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HQU75728.1"
                     /translation="MTLQQAQQLVDEWINTTGVRYFNELTNMAMLTEEVGEVARIIAR
                     RYGEQSEKDSDREKVLADELADVLFVLICLANQTGVDLEEALRRNLEKKSSRDAERHK
                     NNEKLQ"
     gene            8335..10212
                     /locus_tag="PK742_03730"
     CDS             8335..10212
                     /locus_tag="PK742_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75729.1"
                     /translation="MKLWRITLLLFCLLAMPQVKAQLVNAYAKVTSVSGGGTVLNLGT
                     MEETYDTFEDGEFVIIMQMQGATITGGTGAGFGTITDIESAGLFEIGTILTHTASSIT
                     LTAPLSNTYEPIAGSVQVVSYPTMGGGGNYTTTSNLRARKWSGEIGGVIAFNVSGNLY
                     LAHEISADSMGFDWGAKAGTSASTECWYSTYYVKEDGNIHAAYKGGGLQNPLTTQMCG
                     RAPIANGGGGGVGHNGGGGGGGNFTGGGDGDPGTCSALFGGAGAGGHALNSWIPADTI
                     YRIFLGGGGGGGHENNGKGSHGGYGGGIIIIEADSIFADCAGSVKISANASEAPSKKN
                     DGQGGGGAGGSIILYTEYIGATAGCPLSVSADGGDGGDVNHDDDHGGGGGGGQGFIYV
                     EAEEMDLMPMDYLTFSTANGEGGLNCYTCGAADDGEGVDDSGIFFGEAPLLPVGFVYV
                     NAQLLSEQLVSLSWTSGDEFSCERYDIYRSYDGNQWQVIGSVPCVGYGAGSSDYVFVD
                     DQPLKFYNYYIIKQYNSTGRSRNSNMVSVFLDAATNGQLNLYPNPSDGQMVQLHSIHE
                     LSGGKLLITDLQGRHVSAEAEMTGPYDLVITTTGLLPGIYLITLQTARGLISNARMII
                     E"
     gene            10241..11470
                     /locus_tag="PK742_03735"
     CDS             10241..11470
                     /locus_tag="PK742_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU75730.1"
                     /translation="MSGRLQPLRQIEFRRFLAMRSALTIAYQIQAVVCGWEIYRLTGD
                     PLSLGLIGLAEAIPSISIALYAGHLADKSNKRNLLLWTVAAFFLCSGGLALVTSPELQ
                     AAHSTAFTVNAMYALIFLSGFARGFYSPTGFSFIPQLIPKEQLSQATTLNSTIWQFSA
                     IIGPALGGFLYAGFGMTGAMTTVLVFSVLSFVSVARISSKPVVQAGNKEPVADRLREG
                     LRFVFGHKVILNALALDMFSVLFGGAVALLPVFAEDILQVGPQGLGVLRAAPSVGAVL
                     TMSWLSLRKVPGKAGTTLLWAVTGFGVTILLFGISTSFYLSLFLLFLNGAFDSVSVII
                     RANIIQLETPDEMRGRVSAVNSMFIGSSNEIGSFESGLTARWFGAVGSVLFGGSMTLL
                     IVGYTALKAKTLKEYSY"
     gene            11639..>15079
                     /locus_tag="PK742_03740"
     CDS             11639..>15079
                     /locus_tag="PK742_03740"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75731.1"
                     /translation="MRKRTLFFFLCSTHLFFPALLRAQMDCSAHPDDMMMHDEHCAVF
                     ALVPEDGASHVSVSNGSWFAPATWNTGTVPGADATVLIDSGTVVTYDGSSNTEIFTIR
                     VNGTLQFATGINTLLVVNTLVVDPAGIFTIGTAASPVNAAVTAQIIFPDQGAINTMWD
                     PYQFSKGLVSHGSTTMYGAAKKSTISLTGSLAQGVNTLHLTESPLGWQPGDRLILPGV
                     HTNGGGSMEDNSRYEDEVLIISSISGTNVQFEREADGSHSLLYSHDPPPGFGLQPVVG
                     NLTRNIRIEAENAGALPVQQRGHLMLMHNPAQHMNYVYMYGLGRSDKSLLVDDPVVDS
                     MGMLISGGSNPRGRYACHVHQAGRHDHAGEPLTITGCVVEGTPGWGFVHHSSHADLTD
                     NIVFGFKGAGFVAEAGDELGSMVHNLSVKGYGLQDGDYTDLLDRLNAFDLGLEGDGFW
                     MMGSNLSLIDNIATSCSGAGFNYFSDDDDITPNRRLKIPKAALPNPSITGALDSIYAG
                     VTPVRANVGSQAYNVNQGMNFWTHLYNNDNVGDFSRMEYDGLTHNATSTVQNFHIWNI
                     RDKGIAINYSSQLRFLDGILLGDMEHLYTNPDGSLVSENAGSAFVSNGVTGEVQISGC
                     TIAGWQKALIALRTDNLVNGDDLEYNYKTSLISGNTLFNNVVSLVPETGQDAYYVPGV
                     ERFPAYLQLTEDNVFETIASDLPPLADFSYTSAGGLAISFDAGMSADPDPKVSNAGNG
                     IAAYRWQFGDGETGYGKDPLHTFSSAGTYTVTLTVTDCLGQTTDKTKNISVNTEYYSN
                     QIRFSNMEMDSLHDWKLVTSLDRFTSGSWGTGKDWTLMDGKAVIHKSLNDRYLVQVNR
                     NDHLLQGVVPFSIQLRNIGDGASSNSLRVEITGVNGELSDDDPVHPDHISAWNNGDPE
                     FSSTVLLSEEWGLTSYNWQTFSQDIDFGSGYQFLVIKIFSQGLKVSDSDEQGVDNLCL
                     PCYCATPGGLLADDLTDHSATLIWDNVGALNYEVRYRPKGGSWISTEVNNTFMQLTSL
                     LPNKRYQWQVRAECEGSWTAWSAISNFMTPATGSLCTSPTDLSVSWTTANAALVTWES
                     IPGSLLYRVAWRATSGGPWLTQYISDTSVQLSSLLPSTLYNWRVSAQCTEGWKPFLSL
                     S"
BASE COUNT         3808 a         3625 c         3881 g         3765 t
ORIGIN      
        1 atgctcatac tactgcagtc tgagcaagga acagccgttt ccggaaacca gttgtaatac
       61 agagcgccat cagcaaaaat gtccaggctc tcacccaggc agatggttgt gtcattcaaa
      121 gtgaccacct ccgggttggc gtaaatgatc acactaacag tatcagttcc cgtacaggta
      181 ccattgcttg ccgtcaccac gtagtctgtt gtagtactta caaaaacgat cgggtctgca
      241 atattgacat tacttactcc ggtaccagga ctccaggtat aggtatcgcc tccgctggca
      301 aagaactgta tctgatttgc tccgcaaaga gtcgtatcag gtccggccac tacagtaagt
      361 ccgggttcca cctccacgct cacggatacc gtatcaaaac aggcagtgcc attacctaca
      421 atgaccgtat aggtgacaga agcatcgggg aagcaagtag gttctgcaat gtctgttgcc
      481 gataaatagt caggcgggct ccagctgaaa gtggtgccac caccggcggt cagttcaacg
      541 atattgcccg cacaaatggt cgtatcattg ctgatggttg ccatcagcgg tacatagggt
      601 atatcagctt ccagcagagg ccagtcagca atggtctgac cggttgcacc atcggtcgca
      661 ccgtttgttt cattgaagca ggggctgctt gcagtggtag tgataccggc agcacctcca
      721 ccatagtctg tcagaaaacc tgccggcaga gaggcaccag tatggcgcac caatccaccg
      781 ccaccaccac cgcccggacc ggtacaggcg tttccggtaa tattgcttcc gtttcctcct
      841 ttcagttcta ccagcacgtc actgctcagc gtactgacat ccagcaaaag gcttccgcct
      901 gcacctccac caccgccgcc atcgccgttg gctgatgccg gagaaccacc gttggagcgg
      961 atggagaatc cattaccatc cagtgagttg acctgcagaa aaatgatgcc accaccatga
     1021 ccacctgctt tcgcaaatcc gttgttgcca tgtccggcac cgccacctcc acccagccag
     1081 atcctgttct ctgcgttgga atgatcagga agtactccgg gtctgcctgt gccggggccg
     1141 ggacaggaga acagatccac atcacgttca cctccctcac ccccttcacc tcctaaagta
     1201 cctccacctc caccggcatt gtgatcgttt ccgccacctc ctccgttggc cagtttaccc
     1261 cgaccggcac gacgagcatc tgaaacggaa gtaaaacctt cacctttgat ggctccgttg
     1321 ccactggttg tggtggtata gtaaccgttc caggaaaaac caaaggggca gctgtcagga
     1381 aagttggtga actctccgcc cctgtatccc ttttcactga cgtccatatc tgcttccagg
     1441 gtcagtgtgc cgtttacaat cagagccagt atgcctcctg tttcaccgtc ccaggcagga
     1501 caggtcagaa cagaatcgac cagtacatca tcatacacag gaaccctgac aacctgcacc
     1561 cggaagctga aatcatacgt gttctcagtg agatatgcca gttgcagatg atcatctccc
     1621 acatcagcca attcattgag ttcatatttt ccggcggttc cccagttcag gatagtgcca
     1681 aatgatgaag tattggaaga atcaataaca gcacctttca tctgaacaat aaggaccttg
     1741 tcgcccggcg aaaaagcagc agcatcagta acctccagtt tattatcaca tcctatacca
     1801 gtggctatcg tgtagtcatt gattacccca tgaatggcag tttgtgccat gctgaaaatg
     1861 ggcatgaaca agacaaagat caggatatag cggcgcatgg gtatgatttg ccaccgaagt
     1921 tactgacgtt tgttggagaa aaacacaaca ccggcgggta aaatgtaccg cagatctgca
     1981 gcctttctcc gggtaagtat tgcaaattat gtaagttagc agtacatcaa attgtgccat
     2041 caaccacatg aatcaatcta acagatctgt tcccggctgg tattggatac tggccgtctt
     2101 gttcctccta tggaatctga tgggtgtaag ccagttcttc gccattacct tcatttctga
     2161 tgagtcactg gccaggcttt cagtggaaga acgggcttta tatgagagct atccgggctg
     2221 ggtcacggtg gtctttgcgc ttgcggtctt tggaggtagc attgggtcta ttggtttgtt
     2281 acttcgaaaa aggtgggcac gtctggcctt catcatttcg ctgatcggaa tagtccctca
     2341 aatgatccag aatgtcttca tgaccgatgc cagagaggta tatggaccgg ggtcagtggt
     2401 catgcccgtg atgatcctcg tgctgggtat ctttcttgtt tggtatgccg gtatgtgcat
     2461 cagaaagggt tggttgagat aaattcgttt cagcacccat caggtcatgc gatttggtga
     2521 tatatctatt ctgaaatata tcatcagaga tgatcaggat atttcgccgg ctcagaatgc
     2581 aacaacatct gcgaaacgtt gaaaaggcta tggaagtatt ctgccgaatg ctcgaaaggg
     2641 agtgcagata acggtacaag atgttttact gatcttccgg tattcagtgg gccattgtaa
     2701 cctggctgaa atatcctgcg tatatcctga tatgtctttt ggctccatag cagaaatgat
     2761 cgccacactg aaaaggaatg cccgtcccaa aagagaaggg tataaaccgg atcagaaaaa
     2821 gcacctgttc agaagggagg cagatccggc aaagctggaa gaagccaggc aggaaaggga
     2881 aagtcatcga agaaatgccg gtcgtttgcg gatcatcatt tttctgtttc tcatcatatc
     2941 cgttctggca ttgtatctgt tcagttcacg tgtatttgtt tgaaccggca cggagtatca
     3001 ctcttgtatg ctgataggga agcaggtatt tatcagccat ctatccccgg ttccatacct
     3061 ttgcacaaaa cctccatatg tacggttcta tccaacaacg cctgcaacat gaactgcagg
     3121 agatcaaaga cagcggactc tataaaaatg aacgcatcat agcttccccg cagggagccg
     3181 atatcacgct ggctgacggc agtcaggtca tcaatttctg tgcgaacaat tatctcggac
     3241 tcagtgcaca cccgaaggtg atagaggctg ctaaaaaagc gatagatacc catggctatg
     3301 gcatgagctc tgtacgtttc atttgtggaa cgcaggatat tcataaggag ctggaagaga
     3361 agatcgccac gttcttagga acggaagata ccattctcta tgccgcggca ttcgatgcca
     3421 acggtggggt tttcgaaccg ctgttcaatg aagaagatgc catcatcagc gatgccctta
     3481 accatgcatc catcattgac ggtgtgcgtt tgtgtaaggc acagcgattc cgctatgaac
     3541 acaacaatat ggatgatctg gaagccaaac tgaaagaagc ttcgggtgcc cgcaccagaa
     3601 tcatagtaac agatggtgca ttcagtatgg acggaaccat cgcccgcctg gatctgatct
     3661 gtgatctggc agaaaaatat gatgcactgg tcatgataga tgaatgccat gcatcaggct
     3721 tcctcggaaa gaccggtcgc ggaacacatg aacacagggg cgttatgggt cgtattgata
     3781 tcatcaccgg cacccttggt aaagcgttgg gcggcgcttc cggcggattc acttccggtc
     3841 gcaaggagat cattgacatg ctgcgtcagc gctcccgtcc gtacctgttc agcaataccc
     3901 ttgcgcccag tatcaccggt gcttccatcg ccgtgctgga cctgctgagc gagaccaccg
     3961 agctgcgtga taaactggag tcgaacacga agtacttccg cgagaaaatg acggctgccg
     4021 gatttgatat caagcccgga gaacacccta ttgttcctat catgttgtat gacgctgtac
     4081 ttagtcagca gatggcagaa aaactgctgg ccgaaggcat ctatgtgata ggcttttact
     4141 atccggttgt tcctaaagga caggctcgta tccgggtgca gctcagtgcc gcacacgaca
     4201 ggcatcacct ggataaagcc attgaagcct tcatcaaggt gggtaaggag ttgggtgtgg
     4261 tcagctgacc gaaaggttca ttctgaaact aatgggtatc ggtttaaaga tccaatccag
     4321 gagattatgc gcttatacgt taccggtctt ttgttttctg tgttgttgtg caccgggcaa
     4381 ttgtttgcac agcaaaccag agcggcactg tttctgggaa actcctatac ctacgtgaat
     4441 gatctgccca agatgatagc tgatgtagcg ctgaccaccg gagataccct gatctatgac
     4501 agcaatacac ccggcggata tacgctgcag caacacagta cgaacgcaac cacgctggca
     4561 atgatcagta ccggtgcctg ggattttgtc gtgttgcagg aacagagtca gcttcccagc
     4621 ttcccgatcg agcaggtaga agtggaagtg tttccttatg cagcattact ggacagtctg
     4681 atcagccagg caaacccttg caccgagacc gttttttata tgacctgggg aagaaagaac
     4741 ggcgatgcta tgaactgtcc gttctggccg ccggtatgca cctatacagg tatggatgac
     4801 ctgttgcggg aacggtatga gatcatggcc gccgataacc aggcccttgt ttcaccggca
     4861 ggtgctgtat ggcggtacat tcgcgagacc gatccaacca tcgaattgta ctcagcagat
     4921 gaaagccacc cttctgtggc cggcaccttt gccactgcct gtgcattcta ttcggtgatg
     4981 ttcaggaaag accccgtgct tacttcttat gatgccgggc tggatccggt tttggccagt
     5041 accatcagag ctgcagcttc tgcggtggta tttgacagtc ttgatcactg gcatgtaggt
     5101 gaatacgacc ccgttgcagc attcagtgtc tggccgggac cagagggtca tatagaaaca
     5161 gaaaacctgt ctgaacaggc agatacctgg caatggagca tgggtgatgg cacggtgtac
     5221 acagatgcag aacctgctca tacatatgca gaaagtggca gctatatggt tcagctgata
     5281 gcttccaggg gtgattgccc aggcgatacg tcagagcaaa ccattcttgt aacagtatca
     5341 gagccgaacg ttgtgaccaa catgattccg ggactttcct gggtgttgtc aggaaatgtg
     5401 ctgaccctga ccacctccac ggaggcgatc agatacagga tgctggacag cagcggaaag
     5461 gtgctgacaa gcggtaccat tcctgctgcc ggtcatttga accttccgct cgatgaattt
     5521 gctgccggtt tctgcttgct gcagctcaga tctggaaggg gtcagcagat cataccggtt
     5581 ccggtacatt aagtgatccg gcagagttat ccaccattga acataccagt agctcctgaa
     5641 tctttgtagt ttagttgcta tagcaattat ggcaggaaaa aagaagaccg atccggctgc
     5701 cctgttactg ttgatggacc aggcggtcag gcatgccaag caacaactgg tagccgcctt
     5761 gcagagatcc ggattggaat tgacggcaga tcagtggacc ttgctgcgca tagtagtaga
     5821 atctgatggc atagcccagc atgtattggc agaccagtcg ggcaaagaca gaccaacagt
     5881 tacccgcatg ctcagactga tggctgaccg cggctgggtg gtacaggaac ctgcacccgg
     5941 cgacaggcgt tcttttctga tcctgccaac caaagcaggc cagcaggttt ggaaagcagg
     6001 ccaggcagtg gtcaaggaac aactcaggca aacctttcca aaatctacag gtaccctgca
     6061 gaaaagtaca cgcaaattat gcagagagct gcttaagaaa tgagccgaaa tgggcactta
     6121 ttagaccaat aatgtccaat attgcgtggc gacccaagca ttctcttttt ttcatccgga
     6181 ataccaaatc cattaaacca taatggaata ctgcagtcca atgaccatgc aaaaactcct
     6241 gacagccttt acagtatttt tagttgctat aacaattaaa gcccagacag agggcgatca
     6301 gttatttgcc acagaccagg tggtcaccat tgaactgacc tttacacagg agggattctg
     6361 ggacagcctg gtggccaatt atgaaaccgc atcctacatg ctggctgatc tggacatcac
     6421 agaccaaacc ggtacgtatt catttcccca gataggggta cgtctgaaag gcaactccag
     6481 ctatggtcat ccgggtaaca agaaaagttt taagatagac ttcaacgaat atgtgagtgg
     6541 ccagaattac gacggactca aaaaactcaa cttttccaac aatttcaaag acccgacatt
     6601 tctgcgggag aagatctatt ctgacctgtg tctttccaat gacctgcctg ctccccgcac
     6661 ttcctatgct gatgtgtata tgaatggcac ccactggggg ttttatacgg tcatcgaaca
     6721 gatagatgat cagttcctgg actggaacat ggaagatgac ggcggcaata tgttcaaagc
     6781 cggcgatgcg tttgacggtc ccggtggcga agctgacctg gtgtactatg gagctgatca
     6841 gtcttcctat accgaacggt atgagatcga aaacaatgaa gacctcaacg actggacaga
     6901 cctgatagct ttcattgatt ttattaataa caccaccgat gcagagtttg aaacaggtat
     6961 cgatgaccgg cttgaaaccg atcagtatct gcgcagtatg gccatggata acctcttttc
     7021 caatctggat gcctacatca actcagcccg caattattat atctaccatc atgaaggaac
     7081 cggcaggtgg aactggatca aatgggattg caacgaagct tttggttcct accagggcgg
     7141 cccaatgggg ggtggattat ccatgaccga actggacttg cattatgtga acaattcccg
     7201 gcctttgctg gagcggataa tggaaattga cggcatgcag gcccgctttg atgatgcatt
     7261 gtgctactgg atcaacggtg atttttcgtc tgcaaagatc caggaagaaa tagaccgcta
     7321 ttatgaactg atcaaaccct atgtgtatgc agatgaaaac aaaatgtaca ccgatataca
     7381 gtttgaaaat aatatatatg aagatataac tacaggtggc ggaccgggag gtggtaccat
     7441 ttttgggctg atgtctttta tggaagccag gcttgacttt gttgaaggag cgttggactg
     7501 tactgctgtg gtggcgatcg atgagtcatc ggcacacgac gcttttttct gtcggcctaa
     7561 tccggctggt caggtggtat gggtccaaac catagaaccg gaacgcatca cggtatttga
     7621 tctgaagggt cagcaggtat tacaggcagc cagcggttgg gtagatatct ctgcgcttcc
     7681 cgaggggctc tatatattgc gctcggattc gggaaacact acacgtctgt cagtggtcag
     7741 gtagtaaaag aaggcaggta tgccagacgg atttaaaccg gttacaacac cccttcccat
     7801 ttcccagcct gaaggcaggc acatgttaac ttttcacttt ctacttttaa ccccatgacc
     7861 ctgcaacagg cacagcaact ggttgatgaa tggatcaaca ccaccggtgt caggtatttc
     7921 aatgaactga ccaatatggc catgctgacc gaagaagtgg gcgaagtggc tcgtatcatt
     7981 gcccggcgtt acggagagca aagtgaaaaa gacagcgacc gcgaaaaagt gctggcagat
     8041 gagctggccg atgtcctgtt tgtgctgatc tgcctggcta atcagacagg ggtagatctg
     8101 gaagaagcac tgcggcggaa tcttgaaaaa aagtcatcca gagacgcaga acgtcacaaa
     8161 aacaacgaaa aattgcagta gatagcggat caatggccgg tcaatagtgt agcaggcaaa
     8221 gaacagtcca catttttcct gtaacaactc ccggtacctg caaccctttt ggcagtactt
     8281 gcttctatat ttgttgaact tcggctcagt gtctgccggg gtgaacattt gatcatgaaa
     8341 ctctggcgaa tcaccctact gttgttttgt ctgcttgcca tgccgcaggt gaaggcacaa
     8401 ttggtgaatg cttatgcaaa ggtcacctct gtttctggtg ggggtaccgt acttaacctg
     8461 ggtaccatgg aagaaacgta cgacactttt gaagacggtg agtttgtgat catcatgcaa
     8521 atgcagggtg caaccattac cggtggaaca ggtgccggtt ttggaacgat cacagatatt
     8581 gaatctgcgg gtttgtttga gatcggtact attctgaccc atacagcgtc ttccatcact
     8641 cttacagcgc cgctttccaa tacctatgaa ccaattgccg gaagtgtaca ggttgtgtct
     8701 tatccaacta tgggcggtgg cggcaattat accactacat caaatctgag ggccaggaaa
     8761 tggtctggtg aaattggcgg agtgattgcc ttcaatgtat caggtaatct ctatctggcg
     8821 catgagatat ctgcagattc catgggtttt gactggggtg ccaaagccgg tacatccgct
     8881 tcaaccgaat gctggtattc tacttattat gtgaaggaag atggaaacat ccatgccgct
     8941 tacaagggtg gtggattgca aaatccgttg accacccaaa tgtgtggcag agcacctatt
     9001 gccaatggcg gtggtggtgg agttggtcac aacggtggtg gcggtggcgg aggtaatttt
     9061 accggtggcg gagatggtga tcccggaacc tgcagcgcac tttttggcgg agcaggtgcc
     9121 ggagggcatg ctctgaactc ctggatcccg gccgatacga tctatcggat ctttttaggc
     9181 ggcggcggtg gtggtggcca tgagaataac ggcaaaggat ctcatggagg ctacggaggc
     9241 ggaatcatca ttattgaggc cgacagtatt tttgctgatt gtgccggttc tgtaaagatc
     9301 tctgccaatg ccagtgaagc accctcgaag aagaatgacg gacaaggtgg tggtggtgcc
     9361 ggaggttcta tcatcttata tacagaatac atcggggcaa ctgccggttg tccgctgtct
     9421 gtttcggcag acggaggtga tggcggagat gtcaatcatg acgatgatca cggcggcggc
     9481 ggaggtggcg gccagggttt tatctatgtg gaagccgaag aaatggatct gatgccgatg
     9541 gactatctga ccttttcaac agcaaacggt gaaggaggac ttaactgtta tacctgcgga
     9601 gcggccgatg atggagaagg ggttgacgat tccggaatat tctttggcga agccccttta
     9661 cttcctgttg gttttgttta tgtgaacgct caattgctgt cagaacagct ggtaagcttg
     9721 tcatggacca gtggtgatga gtttagttgt gaaagatatg atatctacag atcctatgac
     9781 ggcaaccagt ggcaggtgat cggatcggta ccttgtgttg gctacggagc cggatcttct
     9841 gattatgtct ttgtagatga tcagccatta aagttttata actattatat cataaaacag
     9901 tataatagta caggcagaag ccgcaattcc aacatggttt cggtattcct ggatgcagca
     9961 acaaacgggc agttgaacct atatcctaat ccgtctgacg gacaaatggt acagttgcat
    10021 tctattcatg agctatcagg ggggaaattg cttatcactg atttgcaggg tcgtcatgta
    10081 tctgcagaag cagaaatgac cggcccttat gatctggtca ttaccacaac aggtctgttg
    10141 cccggcattt atctgataac cctgcaaact gccagggggt tgatcagcaa tgcccgaatg
    10201 atcattgaat agtccctaac ttcgccggca aacaaacgct ttgtcaggac gtcttcaacc
    10261 gctcagacaa atagaattca ggcgctttct cgctatgcgg tcggcactca ccatcgccta
    10321 ccagatccag gcggtggttt gcggttggga aatctatcgt ttgaccggcg atccgctatc
    10381 actgggtctt atcggtctgg cagaagccat cccttctatt tccattgccc tgtatgcagg
    10441 tcatctggca gataaaagca acaaacgcaa cctgttgttg tggaccgtag cagctttttt
    10501 tctttgttcc ggcggactgg cactggttac ctcaccggag cttcaggcgg cgcattccac
    10561 tgccttcaca gtaaatgcca tgtatgcact gatctttctt tcagggtttg ccagaggttt
    10621 ctattcacca accggatttt cattcatccc acaactgata ccgaaagaac aactgagtca
    10681 ggcaaccaca ctgaacagca ccatctggca attctcagcc atcatcgggc ctgcattggg
    10741 tggattttta tatgcaggtt ttggcatgac gggtgccatg accaccgtat tggtcttcag
    10801 tgtactgtct tttgtcagcg tagcccgcat cagctccaaa ccagttgtac aggctggtaa
    10861 taaagaaccg gtagccgatc gtttgcggga aggtctgcgt tttgttttcg gccataaggt
    10921 catcctgaat gccctggcac ttgacatgtt ctctgtgctg tttggcggtg cggtggcatt
    10981 acttcccgtt ttcgccgaag acatcctgca ggttggtccg cagggccttg gggtgttgcg
    11041 agcagctcca tcggttggtg cggttctgac catgagctgg ctctcgttgc gcaaggtacc
    11101 ggggaaagcc ggtacaactt tgttatgggc tgttacaggc tttggagtga ccatcctgtt
    11161 gtttggcatc agcaccagtt tttacctgtc gttattcctg ctgtttctca acggagcttt
    11221 tgacagtgtg agtgtgatca tccgggccaa tatcatacaa ctcgaaacgc ctgatgagat
    11281 gcgtggcagg gtctctgctg tcaacagtat gttcatcggt tccagcaatg agatcggttc
    11341 ttttgagagc ggactgaccg cccgctggtt cggagcggtg ggcagtgtgt tgttcggcgg
    11401 aagtatgacc ctcctgattg tgggttacac tgcactgaag gcaaagactc tgaaagagta
    11461 ctcttattga ccgatgcaac ggtttttttt aagtttgcgt ctaatccata ttcccgacca
    11521 gactttcccc ccaacataca ctacctgaac acccgcttcc ccgcaggcaa caggtaagcc
    11581 ttgtgcagca cccacatcaa catgtgacac acacctgaac acatgtaaaa aatcaagtat
    11641 gagaaaaaga accctattct ttttcctgtg cagcacacac ctgttctttc ctgccttgct
    11701 ccgggctcaa atggattgct ctgcgcatcc tgatgatatg atgatgcatg atgagcattg
    11761 tgccgtattt gcactggtgc cggaagatgg cgcatctcat gtttctgtta gcaatggcag
    11821 ttggtttgcg cctgctacct ggaacaccgg aacggttccc ggagcagatg caactgttct
    11881 gatagactcc ggcacagtgg tgacctatga cggcagcagc aacactgaga tcttcaccat
    11941 tcgggtaaat ggtaccctgc agtttgccac aggcataaat accctgctgg tggtcaatac
    12001 actggtggtt gatccggccg ggatctttac cattggtaca gctgccagcc cggtcaatgc
    12061 ggcagtcacg gcacagatca tctttcccga tcagggtgcc atcaatacca tgtgggatcc
    12121 ctaccagttc agcaaagggc tggtttcaca tggatccacc accatgtacg gggcagcaaa
    12181 aaaatcgacg atcagtctga ccggatcact ggcgcaggga gtgaataccc tccacctgac
    12241 agagtctccg ctgggctggc aacccggaga tcggcttatt ttacccggag tgcataccaa
    12301 tggtggcggc agtatggaag ataacagcag atatgaagat gaagtgctga tcatctcgtc
    12361 catttccgga accaacgtgc agtttgagcg ggaggcagat ggaagtcatt ctctgttata
    12421 cagccatgac ccaccgcccg gcttcggact tcaaccggtg gtaggcaacc tgacacgcaa
    12481 cattcgtatt gaagcagaga atgccggggc tttgcccgtg cagcaaagag gtcatctgat
    12541 gctcatgcac aacccggcac aacacatgaa ttatgtgtac atgtatgggt tgggcagaag
    12601 tgataaatct ctgttggtgg atgatccggt ggttgacagc atgggcatgc taatcagcgg
    12661 cggatcaaac ccaagaggcc gttacgcctg tcatgttcat caggcaggaa gacatgatca
    12721 tgccggtgag ccgcttacca tcacaggttg tgtggtagaa ggaacacccg gatggggatt
    12781 cgtacatcac agcagccatg cagatctgac agataatatc gttttcggat tcaagggtgc
    12841 gggttttgtg gcggaagcag gcgatgagct gggttctatg gttcacaacc tgtctgtcaa
    12901 aggttatgga ctgcaggatg gtgattacac tgatctgctg gaccggctga acgcctttga
    12961 tctgggtctg gaaggtgatg gtttctggat gatgggcagc aacctttctt tgatcgacaa
    13021 tatagcaacc tcctgttcag gcgcaggttt caattatttc agcgatgatg atgacatcac
    13081 accgaatcgc cgcttgaaaa taccaaaagc tgccttgccc aacccttcca ttacaggtgc
    13141 actggatagt atctatgccg gtgtgactcc ggttcgtgcc aatgtgggtt cgcaggcata
    13201 taacgtaaat cagggcatga acttctggac ccacctgtac aacaacgaca atgtgggtga
    13261 tttttcacgg atggaatacg acggccttac ccataatgca accagcacag tacagaattt
    13321 ccatatatgg aatattcgcg acaaaggaat tgccatcaac tattcctcac aactgcgttt
    13381 cttagatggc attctgctgg gtgatatgga gcatctctat accaaccctg acggctcact
    13441 ggtgtcagaa aatgccggtt ctgcatttgt cagcaatggg gtaaccggag aagtacagat
    13501 atcgggttgt accatcgccg gatggcaaaa agccctgata gcactgcgta cagataatct
    13561 ggtgaatgga gatgacctgg agtacaatta caagacctct ctcatctccg gtaatacctt
    13621 gttcaacaat gtggtaagcc tggtgccgga gaccggacag gatgcttatt acgttcctgg
    13681 tgtggaacgt ttcccagcct atcttcagct tacagaagac aatgtcttcg aaacaattgc
    13741 ttcagatctt ccgccgctgg ctgatttcag ctatacctct gcaggtggcc tcgctatcag
    13801 ctttgatgcg ggcatgtcag ctgatcccga tccaaaggtt agcaatgccg gaaacgggat
    13861 cgcagcttac agatggcagt ttggagatgg agaaaccggg tatggaaaag accctttgca
    13921 tacgtttagt tcggcgggca cctatactgt taccctgacg gtgacagact gcctgggcca
    13981 aactacagat aaaacaaaaa acatatcagt aaacacagaa tattacagta atcagatcag
    14041 gttcagtaac atggagatgg atagtctgca cgactggaag ctggtaacca gtcttgatcg
    14101 gttcacttcc ggcagctggg gaaccggaaa agactggacc cttatggacg gaaaagcggt
    14161 cattcataaa agcctcaacg accgctatct ggtacaggta aacagaaatg atcatctgtt
    14221 acaaggtgtg gtgccatttt ccattcaatt gcggaacatc ggagatggcg catcatccaa
    14281 ttcattgcgg gtggaaatta ccggcgtgaa cggcgaactt tctgatgatg atccagttca
    14341 tccggatcat atttctgcct ggaataatgg tgatccggaa ttcagctcca ctgtattgct
    14401 gtcagaagaa tggggcctta cttcttacaa ctggcagaca ttctcacagg atattgactt
    14461 tggatcgggt tatcagtttc tggttatcaa gatcttttct caggggttga aggtgagcga
    14521 cagtgatgag cagggtgtag ataacctgtg tctgccttgt tattgtgcca ctcccggcgg
    14581 gctgctggca gatgacctga cagatcacag tgctacgttg atatgggaca atgtgggtgc
    14641 tctgaattac gaagttcgct acagacccaa aggaggcagc tggatcagta cagaagtgaa
    14701 caatacattt atgcagctca cttcactcct tccaaataaa cgttatcaat ggcaggtgcg
    14761 ggctgaatgt gaaggcagct ggacagcctg gtctgcaatc agcaatttca tgacaccggc
    14821 aaccggcagt ttgtgtacat cgccaacaga cttgtctgta tcatggacca cagcaaacgc
    14881 agctttggta acctgggaaa gtattccggg ttcattgctg tacagagttg cctggagggc
    14941 caccagcgga ggcccatggt tgacccagta catttctgat acgtctgttc agttgtcttc
    15001 cctgttgcct tccactttgt ataactggag ggtgagtgct cagtgtactg aggggtggaa
    15061 gccattctta agtctttca
//
LOCUS       DAOMAX010000041        15012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_462_length_15012_cov_4.607140, whole genome shotgun sequence.
ACCESSION   DAOMAX010000041 DAOMAX010000000
VERSION     DAOMAX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15012
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_15012_cov_4.607140"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PK742_03745"
     CDS             complement(<1..1114)
                     /locus_tag="PK742_03745"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU75732.1"
                     /translation="MAIVILIIYVLAITLLVLYCLTQLSLAIHYLNYKKRLRDEQHLE
                     PLKMEDAATFPTVTVQLPIYNELYVIERLIDAVAVFEYPANKLDIQVLDDSTDETVDV
                     VAARVAYWKEKGVQIEQVRRVDRKGFKAGALQEGLKTAKGEFVAIFDADFIPASDFLL
                     QTVPYFNDDKVAVVQTRWEHLNKDYSILTKIQAFALDMHFRVEQSGRNSAGYFMNFNG
                     TAGLWRVAAIQDAGGWMSDTLTEDLDLSYRAQLKGWKFRYVETIASPAELPTDMNAVK
                     SQQYRWNKGGAETARKIGYRVLVAQLPLKVKLHAIAHLFNTTNYIFIFLTAILSVPLL
                     LIKNTYVDINFFRYASVFLIGSISIGFAYFIATRQGE"
     gene            complement(1114..1803)
                     /locus_tag="PK742_03750"
     CDS             complement(1114..1803)
                     /locus_tag="PK742_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU75733.1"
                     /translation="MKVKVIIPVFNESGSIGHVLTDIPRDIVNEVIVVNNGSTDDSPE
                     IAASAGATVLSEPVKGYGGACLKGLRYLESLPVDQQPDIVVFMDGDYSDYPEETAVLI
                     EPIVAGKADMVIGSRALGQREKGSMMPQQIFGNWLATTLMRWFFGARYTDLGPFRAIT
                     WSGLQKLEMQDTNYGWTVEMQIKAVKRGLRYTELPVSYRKRIGKSKITGTVKGTVMAG
                     YKIIGTIIKYA"
     gene            complement(1876..3246)
                     /locus_tag="PK742_03755"
     CDS             complement(1876..3246)
                     /locus_tag="PK742_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQU75734.1"
                     /translation="MSEVNSSLSLANPHPANYNGTQKLGLALIAIGVLSLALAWVGIG
                     KDQALYFFIAMLAGLMGGGLIYFYGTYGKLPAGIKNNRVFFSSIASRGALGWMLGIIL
                     TGFYISLYFFPKYLNGLISLFDPLSQLVRGKDSDQWFVYGTFYTIAVLVMGIKFMMKY
                     RHNRYQLIRTSSVTFFQLILAWTLPIIMENLYNYGPYLSYFWPLDYDAIFPGSLSYVL
                     KSGKLGQFVVVWGLIMTFVATPILTYFFGKRWYCSWVCGCGGLAETAGDPFRHLSDKS
                     LRAWKIERWLIHSVLAFIIILTVLLLVDNAATGKVLGDAAGKSLAKTYSFLIGSIFAG
                     VIGTGFYPILGNRAWCRFGCPMAAYMGIIQRFKSRFRITTNGAQCISCGNCSTYCEMG
                     IDVRWYAQRGQNIVRASCVGCGICSAVCPRGVLKLETGPENAQRTGIQLDSHGLIPLE
                     AVKILD"
     gene            complement(3256..4593)
                     /locus_tag="PK742_03760"
     CDS             complement(3256..4593)
                     /locus_tag="PK742_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQU75735.1"
                     /translation="MHIVIIGNGISGITAARTLRKHSDHEITVISGETDHFFSRTALM
                     YIYMGHMRYQETKPYEDWFWSKNRINLKKAWVKQIDTDNNHILFSDDSGMHYDRLLIA
                     SGSKSNKFGWPGQDLDGVQGLYSFQDLEAMERTTKHGISRAVIVGGGLIGIEMAEMLH
                     SRHYPVTLLVREQSYWNNVLPAQESAMITRHIREHGFDLRLGVELKEILDDGTGKVKG
                     VVTSEGETIDCQLVGLTAGVSPNIEFVKSSKIVCGRGIIVNQFQQTNINGVYAAGDCA
                     QMETPPADRRPIEQVWYTGKIQGETAALNILGRPTAYNPGYWYNSAKFLDIEYQTYGM
                     VLPVLREGEEAFYWEDANGKRSIHLVWRKQDRRFIGINNFGIRLRHTTFNHWLKQQAT
                     VDEVLRDIRAAAFDPEFFKPFEKELISAWNIQFPDHQIQLNNKRSLRARIFHS"
     gene            complement(4733..6727)
                     /locus_tag="PK742_03765"
     CDS             complement(4733..6727)
                     /locus_tag="PK742_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQU75736.1"
                     /translation="MKKLLIGSLILLTFQTQAQNMKPLAALVTSSYNEATAMHPVLFS
                     ESVASPARQASGFDAFAADATFMVLDPAAQADLYALRPASFSMEVMVHGEPVKLLLVQ
                     QNILAEDFRVTTSSHPEGENYSPGVYYRGIIQGDETSLAAISVFEDGLMGLFSGDLWG
                     NVNIGPYTAPDARSMEYVVLAERDLLVNDPFSCATPENSGPETFTNPSGTRTINVPQV
                     YIEADYQLYQNKGSVSNTVNYLTGVFNNSATIYANDGITVENSEIFVWTSDDPYSEGS
                     SFAALSSFQSYRTVFNGDVAHLCALDAGGLGGVAATIDGLCNSNKYCYSDIDASYSDF
                     PTYSWTVMVFTHELGHLFGSHHTQWCGWPGGAIDNCYSTEGGCGPGPAPVSGGTIMSY
                     CHLTGYGINFSNGFGPLPADAIVSTIETAPCLDGGGVSEEYCVAGGSNASEEWIDRVR
                     LGSINRVSGSDGGYYDGTSMSVNMKQGMSKYMRVSAGMAGGPYTEYWKVWIDFNQDLD
                     FDDPGEEIFSLASTLTSNIPVLVSIPADASTGSTRMRITMKYGSAPAACELYAYGETE
                     DYTINITPMLPEALIAAEPFVVSPNPVADLLGFYWEEMAGEDLQVVITSLTGQVVASF
                     SQPAFSGACSMQVNHIPAGIYAIQATTSNGMELVQQFIKQ"
     gene            complement(6825..7934)
                     /locus_tag="PK742_03770"
     CDS             complement(6825..7934)
                     /locus_tag="PK742_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQU75737.1"
                     /translation="MQRSWFTYRNFLTVLGLLFAVWFIWNFYLLIGYFFIAGVVSLLG
                     RPVVERMDKIRLGKFRIPRAFSALTAMLVIFGLLATVVAVFVPLLQDQAEIISSIDTQ
                     KVADSLEEPLLRMEDFIVQLQGGDEGTSLEGLIETKLSEWISVTSLSNILSSILNSLG
                     SIAVALFSIVFISFFFLRDEHLFYEIIMTATPTRMEEKMKNILRGSKIALTRYFGGII
                     IQSTVVILLVTTGLWIMGFPYALLIGFFCGIINIIPYLGPIIGTLFALLVTVTTTLNV
                     HPDANLTIIIIKIVVLIQGVQLLDNYVSQPIIFSKRMKAHPLEIFFIVLIFGTLAGIP
                     GMIMAVPAYSFIRLIAREFFSEFKIVQRITENMEQ"
     gene            complement(7950..8561)
                     /locus_tag="PK742_03775"
     CDS             complement(7950..8561)
                     /locus_tag="PK742_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HQU75738.1"
                     /translation="MSERSKDALILMVKNPVAGKVKTRLAKDLGNEQALEVYQALLQQ
                     TAKVASEVPADRFVFYSDVVDRNDLFRQELFRKYTQCGGDLGVRMEYAFSIPFKNEYK
                     RAVIIGADCPDLDAATISEAFSRLDDHDFVLGPAADGGYYLLGMKKWHRWMFAGQPWS
                     QPELLESTRQTIGSHNMTLAELKTLNDIDTLEDLRQSTFSHLS"
     gene            8670..11381
                     /locus_tag="PK742_03780"
     CDS             8670..11381
                     /locus_tag="PK742_03780"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQU75739.1"
                     /translation="MDRFSYIANAEPAYIESLYRSYKQDPADVDAEWGKFFEGFDFAQ
                     ESFGTNGQAALDADEFKVYNLILAYREKGHLIAKTNPIRPRKDRNAQLQLQQLGFSEK
                     DLSRTFHAGKLIGMENATLADIITRLERVYCSSMGFEYDYIIDPEEKEWWRSRIEEGK
                     DHIDFPDDKQRRILEKLNQTVVFEQFLDKKYIGQKRFSLEGGETTIPALDAIINKGAE
                     LGVEEFVIGMAHRGRLNVLANIMGKTYEHIFNEFEGNVSPDLTMGDGDVKYHLGFSSM
                     VTTSGGHTVHLKLSPNPSHLEAVNPVMQGFVRAKADIMYEGNHQRILPVTIHGDAAVA
                     GQGVVYEVLQMSNLSGYRVGGTIHFVINNQIGFTTDFDDARTSNYCTSYASTIQAPVI
                     HVNGDDAEAVVYAVEMATAYRMQFQKDVFIDMLCYRKHGHNEGDDPKYTQPHLYKLIA
                     NHPNPREVYVQKLLQGHEIQQEMAKSLEKDFWDELQERLDYVKQHNLPYKYQEPEQAW
                     RQLRKNVGPEDFQSSPKTAISKKTLDQVADGIFSVPEGFKILRKVQKLFDSSQNMLRN
                     GQLDWALGELLAYGSLLAEGHDVRLSGQDVRRGTFSHRHSVIRDEESDELHNRLNAIQ
                     NRKGEFRIFNSLLSEFGVLGFEFGYSMASPDPLVIWEAQFGDFSNGAQTIIDQFISSS
                     LSKWQRMSGLVMLLPHGYEGQGPEHSSARLERFLQLCAEYNIVVSNTTTPANFFHLLR
                     RQLKWDFRRPLIHMSPKSLLRHPLVISDVKDITSGTFREVIGDDFADAKQVKRVLLCT
                     GKVYYDLLQYQQEHKRNDVAIVRLEQLYPFPMTQLEETLKQYGHAPMVWVQEEPMNGG
                     AWFFIHNILADRGITRISRKASASPASGFMKVHLKEQQCIVEEAFKL"
     gene            11392..12663
                     /gene="odhB"
                     /locus_tag="PK742_03785"
     CDS             11392..12663
                     /gene="odhB"
                     /locus_tag="PK742_03785"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQU75740.1"
                     /translation="MSIQLKVPSVGESVTEVTLSKWLVADGDYVEIDQSICEFESDKA
                     TLEFPAEKAGRISFKVEEGSDLKIGDVIAELDTDAKAPAKKAVEKEVVSATKETAKTG
                     SKEGSEEQKTNIEKRSSTNDTYAKNHPSPAAQKLAAENHVPLAKINGSGKDGRITKAD
                     VLQAVKDGVSASGAVEMMKRNSREEKAEKVSKLRKTIARRLVSVKNETAMLTTFNEAD
                     MSAIMELRTKYKVAFEKKHGVGLGFMSFFVRAVSNALEEFPAVNAYWNEDQVIYHDYV
                     DISIAVSTPRGLVVPVLRNAESMSMAGIELGIKELAAKGREGTLGLEEMEGGTFTITN
                     GGVFGSLMSTPIINAPQSAILGMHKIQERPVAMNGQVVIRPMMYLALSYDHRIIDGKE
                     SVSFLVRVKEQLENPQQLLFGKDPVDILLDM"
     gene            12678..14081
                     /gene="lpdA"
                     /locus_tag="PK742_03790"
     CDS             12678..14081
                     /gene="lpdA"
                     /locus_tag="PK742_03790"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQU75741.1"
                     /translation="MNKYDVVVIGSGPGGYIAAIRCAQLGMKTAIIEKYDTLGGTCLN
                     VGCIPSKALLDSSEHYHNAAHSFEKHGIGVSGLSVDFSAMIARKAGVVKQTTDGINFL
                     MKKNKIDVYHGHGSFESRNTIAIAKTDGKTDKIEADKVIIATGSKPASLPFITIDKKR
                     IITSTEALSLDKLPKSMIVLGGGVIGCELGSVYARLGTEVTIVEYMDNLIPTMDADLG
                     KDLQRSLKKMGMKMQLSTKVTAVKATTKQVTLTAENKKGESIEMKADYCLVSVGRKPY
                     TDKLGLDNVGIELNERGQIPVNEKLETKVAGIYAIGDVIQGAMLAHKAEEEGVFVAET
                     MAGQHPHVNYLLIPGVVYTWPEVASVGYTEAQLKEKGTAYKTGKFPFMASGRARASMD
                     TDGFVKVLADAGTDEILGVHMIGPRAADMIAEAVVAMEYRASAEDIGRMSHAHPTYTE
                     AFKDACLAASENRALNI"
     gene            14085..>15012
                     /locus_tag="PK742_03795"
     CDS             14085..>15012
                     /locus_tag="PK742_03795"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75742.1"
                     /translation="MKKYIFPMLIAGLAFSCQAQDDKRAGILFYNVENLMDTIDNPVK
                     IDDEFVPTSEKQWNTERYQTKLDHIGQVIASTGVQLPVIVGLAEIENRFVLEDLVKEA
                     HMQAGHYGIVQFDSPDERGIDCALLYDQQRFKVLDAYPVPVYLPKDNGGSTRDILYVT
                     GKFNGTDEVVHIFVNHWPSRYEGEEVSRPRRIMAAQVLRHQVDSLNDLYSDPAIIIMG
                     DFNDTPFDESVMVTLGAVTPDGHYTDESLVNLVANFQKDNVGSYNYKGNWQALDQLIV
                     SESILTDDAAFRVDDRKVRFIRNDFQMYHSDKY"
BASE COUNT         4120 a         3497 c         3714 g         3681 t
ORIGIN      
        1 cttctccctg acgggtggca ataaagtagg caaagccaat gctgatggat ccgatcagga
       61 aaacactggc atacctgaag aagttgatat ctacataagt gttcttgatg agcagcagcg
      121 gaacactcag gatagctgtc aggaagatga aaatataatt ggtcgtattg aaaaggtggg
      181 cgatggcatg cagcttgacc ttgagaggca gttgtgcaac gagaacacga taacctatct
      241 tacgggcggt ttccgcacca cctttattcc agcgatattg ctgcgatttc acagcattca
      301 tatctgtagg tagttcagct ggtgaggcga tggtctccac atatcggaat ttccatcctt
      361 tcaactgcgc acgatagctg agatcgaggt cttcggttag ggtatcactc atccatccgc
      421 cagcatcctg aatggcagcc acacgccaga ggcctgcagt accattgaag ttcatgaagt
      481 agccggcaga attcctgccg ctttgttcca cacggaaatg catatccagc gcaaaggcct
      541 ggatcttggt caggatgctg tagtctttgt tgaggtgctc ccagcgggtt tgaactacgg
      601 ctaccttatc atcattgaag tatggaacag tttgcagcag gaaatctgat gcaggaataa
      661 aatctgcatc gaagatggct acaaactcac ccttggcggt cttgaggcct tcctgcagtg
      721 cgccagcctt gaagcccttc cggtcaaccc tgcgcacctg ttctatctgt acaccttttt
      781 ctttccagta ggctacacga gctgccacca catcaactgt ttcatcagtg gagtcatcaa
      841 gaacctggat atcgagttta ttggcaggat actcaaacac ggctactgca tcaatgagcc
      901 gctctataac atagagttca ttgtagatag gcagttgtac cgttaccgtt ggaaaggtgg
      961 cagcatcttc catcttcaat ggttccaggt gctgttcatc acgcagtcgc ttcttgtagt
     1021 tcagatagtg aatggccagg ctaagctgtg taagacagta tagcaccagc agggtgatag
     1081 caagcacgta aatgatcagt atgacaatgg ccatcaggcg tatttaatga tcgttccaat
     1141 gattttatat ccagccatca cagtaccttt caccgtacca gtgatcttgg actttccaat
     1201 tctttttctg tagctgacgg gtaactcggt atatctcagg ccgcgtttta cagctttgat
     1261 ctgcatctct acggtccagc cgtaattggt gtcttgcatc tccagctttt gcagcccgct
     1321 ccaggtgatg gcccggaatg gacccaggtc agtatatcgg gctccgaaga accagcgcat
     1381 cagcgtagtg gcaagccagt tgccaaagat ctgttgcggc atcatactac ctttttctcg
     1441 ctgcccgagt gctctgcttc caattaccat atctgcctta ccggcaacga tgggttctat
     1501 gagtacagca gtttcttccg ggtaatctga ataatcgcca tccatgaaaa ccacaatatc
     1561 cggctgttga tcaaccggta atgattccag gtagcgcagc cccttcaggc aagcacctcc
     1621 gtatccctta acaggttcag aaagaacggt ggctccggcc gaagcagcta tctcaggaga
     1681 atcgtcggta gaaccattgt tcaccacgat cacttcattg acgatatcac gcggaatgtc
     1741 agttaatacg tgacctattg acccgctttc attgaatacc gggatgatca ctttcacctt
     1801 catgcacgcc tgaatctgcc ttggtttgaa accgagtgca aaggtacaag aataacatgg
     1861 gatataggtc agcccttaat ccagtatttt aacggcttcc aaagggatca gaccgtgact
     1921 gtccagctga atgccggtac gctgtgcatt ttcaggaccg gtttccagct tgagaacccc
     1981 tcttgggcaa acagcagagc agataccaca gcctacgcag gatgccctga cgatattctg
     2041 gcctctttgt gcataccagc gcacatcaat acccatttca caataagtgc tgcagttacc
     2101 gcacgaaata cactgtgctc cgttggtggt gatgcgaaag cgtgacttga atcgctggat
     2161 gatacccata taagctgcca tcgggcaacc aaagcgacac caggccctgt ttcccagaat
     2221 gggataaaat cctgttccta tgacccctgc aaagatgctg cctatcagaa agctgtaggt
     2281 tttggccagt gattttccgg cagcatctcc cagtacttta cctgtggctg cattatctac
     2341 cagcagtaac actgtcagta tgatgataaa agcgagaaca gaatgaataa gccaacgttc
     2401 gatcttccag gcacgcaatg atttatcaga caggtggcgg aacggatcac cggccgtttc
     2461 tgccagcccc ccgcatccac atacccagga gcaataccat cttttaccaa agaaataggt
     2521 gaggatgggt gtagccacaa aggtcatgat gagcccccat accaccacga attgtcctaa
     2581 tttgccgctt ttcagtacat agctcagaga accggggaag atggcatcat aatcaagcgg
     2641 ccagaagtaa gacagatacg gaccatagtt atacaggttt tccatgatga tgggaagggt
     2701 ccaggcgagg atcagctgga aaaaggtgac tgaagatgtt ctgataagct gatacctgtt
     2761 gtgccggtat ttcatcatga atttgatacc cattaccagc acggcaatag tataaaaggt
     2821 gccataaacg aaccactgat ctgaatcctt tccacgcaca agctggctaa gcggatcgaa
     2881 tagtgagatc agaccgttca ggtatttggg gaagaaatac aaactgatat aaaaaccggt
     2941 caggatgata cccaacatcc aacccagcgc tcctctgctg gcgatggaag aaaaaaagac
     3001 ccggttattc ttgatgccgg cgggcagttt tccataggtg ccgtaaaaat aaatgagacc
     3061 accgcccata agaccggcca gcatggcgat gaagaaatat aaggcctggt cttttcctat
     3121 acctacccag gccaaagcca gtgacagaac accgattgct attaatgcca ggcccagttt
     3181 ctgggttccg ttatagtttg cgggatgcgg attggccagt gacaggctgc tgtttacttc
     3241 tgacatatac tgttattatg aatggaatat tctggcacgc agactgcgtt tattgttcaa
     3301 ttgtatctgg tgatcgggga attgaatgtt ccaggctgag atcaattctt tttcaaacgg
     3361 cttaaagaac tccggatcaa atgcagcagc cctgatatcc cgcagaactt catcaacggt
     3421 tgcctgttgc ttcagccagt gattgaaggt ggtatgcctt aaacgtatgc cgaaattgtt
     3481 gataccaatg aagcgcctgt cttgttttct ccaaaccagg tgaatggagc gtttcccgtt
     3541 ggcatcttcc cagtaaaacg cttcttcacc ttcacgcaaa acaggcaaaa ccataccgta
     3601 ggtctggtat tcgatgtcca ggaatttggc actgttatac cagtatccgg gattgtatgc
     3661 tgttggtctt cccagaatgt tcagtgcagc agtttctccc tggatcttcc cggtgtacca
     3721 tacctgttca atgggccgtc ggtcggcagg gggtgtttcc atctgggcac agtcgccggc
     3781 ggcatacact ccattaatat tcgtttgctg gaactgattc acaatgatgc ctctgccgca
     3841 aacgatctta ctgcttttga cgaattcaat attcggactc acgccggccg tgaggccaac
     3901 caactgacaa tctatggtct ctccttcgct ggttactact ccttttacct tgcctgttcc
     3961 gtcatccaga atctccttga gctctacacc aaggcgaagg tcgaagccat gttcacgtat
     4021 atgcctggtg atcattgcag attcctgtgc cggtaataca ttgttccagt atgattgctc
     4081 tctgaccagg agtgtgaccg gatagtgcct gctgtgcagc atttcagcca tttcaatacc
     4141 gatcagtcct ccgcccacaa tgacggctct gctgatgcca tgtttcgtgg tgcgttccat
     4201 agcttcgaga tcctgaaaac tatagagccc ctgtacgcca tccagatcct gtcccggcca
     4261 gccgaattta ttcgactttg atccgctggc aataagcaat ctgtcatagt gcatgccaga
     4321 atcatcagaa aataagatat gattattgtc agtatctatt tgttttaccc aggctttttt
     4381 aaggttgatc ctgttcttac tccagaacca gtcttcgtat ggcttggtct cctggtagcg
     4441 catatgcccc atgtagatgt acatcaatgc ggtgcgggaa aagaaatgat ctgtttcacc
     4501 agagatgaca gtgatttcat ggtcactgtg tttgcgcagt gtgcgggcgg cagtgatacc
     4561 tgagatgcca ttgccaatga ttactatgtg catagaaagc gggatacttt ggacgggtaa
     4621 acttaacaaa gaaatggaaa gcctgtttca atagttccat agatccatca aagttttgta
     4681 aataaaaaag gccggcaaat gtgccggcct ttttatcagg tgctgaaagg cgtcattgtt
     4741 tgatgaattg ctgaaccagt tccatgccat tggaagtggt ggcctgtata gcatagatgc
     4801 ctgcaggaat gtggttgacc tgcatggagc aggcaccgga aaatgcaggt tgcgagaaag
     4861 aggcaactac ctgtccggta agggaggtaa tgaccacctg aagatcttcg ccggccattt
     4921 cttcccaata gaagccaagc agatctgcta ccggattcgg gctaactaca aatggttctg
     4981 ctgcaatcag tgcttcaggt aacatgggtg tgatgttgat agtatagtct tctgtttcgc
     5041 cgtaagcata taattcacat gctgcaggag cagagccata cttcatagta atgcgcattc
     5101 gtgtgctgcc ggtagaggca tcagcgggaa tgcttaccag cacaggaatg ttgcttgtta
     5161 aggtggaagc caggctgaaa atttcttcac ccggatcatc aaaatcaaga tcctgattaa
     5221 aatcgatcca gaccttccag tattctgtat aggggccgcc tgccattcct gcactgactc
     5281 gcatgtattt actcataccc tgcttcatat taacagacat ggaagtgccg tcatagtagc
     5341 cgccatcact gccagacacc ctgttgatgg atcccagacg aacgcggtct atccattctt
     5401 ctgaagcatt ggatcctccg gcaacacagt attcttctga aactccgcca ccatccagac
     5461 agggtgctgt ttcaatggta gaaacaatgg catcagcagg taatgggcca aacccattac
     5521 tgaaattgat gccgtagccg gtaaggtgac agtaactcat gatggttccg ccagataccg
     5581 gtgcaggacc ggggccgcaa ccgccttcgg tagaataaca attgtcaata gcacctccgg
     5641 gccagccaca ccattgtgta tggtgcgagc caaaaagatg tccgagttca tgggtgaata
     5701 ccatgacggt ccatgaatag gtagggaaat ctgagtaaga ggcatcaata tctgaataac
     5761 agtatttatt gctgttgcac aatccgtcaa tagtagcagc cacgccacca agaccgccgg
     5821 catccagggc acacagatgg gcaacatcgc cattaaaaac ggtgcgatag gactggaagg
     5881 aagacaaagc tgcaaatgaa gagccttctg agtaggggtc atcacttgtc cacacaaaga
     5941 tctcagagtt ctctacagtg atgccatcat tggcatagat cgttgcagag ttgttaaaga
     6001 ccccggtcag gtaattgacc gtattggata cacttccttt gttttggtag agctgatagt
     6061 cagcttctat gtacacctgg ggcacattga ttgttcttgt gccagaggga tttgtaaatg
     6121 tttcaggccc tgaattctct ggagtggcac aggaaaaagg atcattcacc agcaggtcac
     6181 gttcagccag taccacatat tccatagatc gggcatcggg agctgtatag gggccaatgt
     6241 tcacatttcc ccacagatcg ccggaaaaca gacccatcag tccgtcttcg aatacactga
     6301 tcgccgccag agatgtttca tcgccctgga tgatgccacg gtagtaaaca cccggtgaat
     6361 agttttcacc ttccggatga gatgaggtgg ttacacggaa atcttctgcc agaatattct
     6421 gctgcaccag caacagtttt accggctcac cgtgcaccat tacttccatt gaaaatgagg
     6481 caggtcgaag tgcatacaga tctgcctgcg cagcaggatc gagtaccata aatgtggcat
     6541 ctgctgcaaa agcatcgaaa ccggaggctt gtctggctgg agatgcaact gactcagaga
     6601 aaagaaccgg atgcatggca gttgcttcat tgtaggaaga tgtcaccagc gcagccagtg
     6661 gtttcatgtt ctgagcctgt gtctggaagg tgagcaggat caaggatcca attagtaatt
     6721 ttttcatatt tcctgattga aggttattac ctcaaaaata caagttagca agcaggcaac
     6781 ctgttgtcag atatgaataa ttacagcagt atgtgacagt ctgatcactg ttccatgttt
     6841 tctgtaatcc gttgaacgat cttgaattcg ctgaagaatt cgcgggctat gagtctgatg
     6901 aaactatagg ccggaacagc catgatcata cctggaatac cggctaatgt accgaatatg
     6961 agcacgatga aaaatatctc caggggatgt gctttcattc gtttgctgaa aataatgggc
     7021 tgtgaaacat aattgtccag caattgaaca ccctggatca gtaccacgat cttgatgatg
     7081 atgatggtca gatttgcatc cggatgcaca tttaaagtgg ttgttactgt aaccagcaaa
     7141 gcgaataagg tacctatgat aggccccagg tagggtatga tattgatgat gccacagaag
     7201 aatccgatca acagggcata gggaaacccc atgatccaga ggccggtggt taccagcaga
     7261 atgaccaccg ttgactggat gatgataccc ccaaagtatc tggtcagggc gatcttactg
     7321 cctcgcagaa tgttcttcat tttttcttcc attctggtgg gcgtagctgt cataatgatc
     7381 tcgtagaaca agtgttcatc tcgcaggaaa aaaaatgaaa tgaatacgat actgaacagc
     7441 gctacagcaa tgcttcccag gctgttgagg atactgctca gtatgttgga gagggaagtg
     7501 acactgatcc attctgaaag ttttgtttca atcagtcctt ccagagaagt gccttcatcg
     7561 cccccctgca gctgtacaat gaaatcttcc attctcagca aaggctcttc caggctatcg
     7621 gccactttct gtgtgtcaat ggaagaaatg atctctgcct ggtcttgcag cagcggaaca
     7681 aaaacggcta ctacggttgc cagcaggcca aagatcacca gcatggctgt cagagctgag
     7741 aatgctcttg gaatgcgaaa ttttcccagt ctgattttat ccattctctc cactaccggt
     7801 cggcccagaa gtgagaccac tccggcaatg aagaaatatc caataagcag atagaaattc
     7861 cagatgaacc agacggcgaa taacagtccg agtacggtca ggaaattgcg gtaagtaaac
     7921 caggatctct gcataaaaag aaaccgaatt taagaaaggt ggctgaaggt gctttggcga
     7981 aggtcttcca gtgtgtcaat gtcgttcagg gtttttagct ctgccagagt catattgtga
     8041 ctgccgatcg tttgccgggt ggattccaga agttcaggct gactccaggg ctggcctgca
     8101 aacatccatc ggtgccattt cttcataccc agcagataat aacctccatc tgctgcaggg
     8161 ccgagcacaa aatcatggtc atccagtctg gagaaggctt cgctgatggt tgctgcatca
     8221 aggtcagggc aatcggcacc aatgatcaca gcccgtttat attcattttt aaaagggatg
     8281 gagaatgcat attccatccg caccccgaga tcgcctccgc actgggtgta ttttctgaac
     8341 aattcctgac ggaacaggtc atttcggtca accacatcgc tgtagaatac gaaacggtcg
     8401 gcaggaacct ctgaggcaac ctttgccgtt tgctgcaata aggcctggta cacctccagt
     8461 gcctgttcat ttcccaggtc tttagccagg cgggtcttca cctttccggc caccgggttt
     8521 ttcaccatca ggatcagtgc gtctttagat cgttcgctca tgccactaca gatttcgact
     8581 taatcggtca accaaatatc caaataatgg gttaatgata gcggaaaccc cttaaaacta
     8641 ccgatatttg caccctgaat cagcgacgca tggacagatt ctcctacatt gccaatgccg
     8701 aacccgccta tattgaatcc ctctaccgtt cttacaaaca agatccggcc gatgtagatg
     8761 cagaatgggg caaattcttc gaaggttttg actttgccca ggaatcgttt ggcaccaacg
     8821 ggcaggctgc actggatgca gatgaattca aggtgtacaa cctcattctg gcctatcgcg
     8881 agaaggggca tctgatagca aagaccaacc cgatcaggcc tcgaaaagac cggaatgcac
     8941 agctccagct gcagcagctt ggtttttctg agaaagacct gagccggact tttcatgccg
     9001 gaaaactcat aggcatggag aatgctaccc tggctgatat cattacccgc ctggaacggg
     9061 tgtattgctc ttccatgggt tttgaatacg attacatcat cgacccggaa gaaaaagagt
     9121 ggtggcgcag cagaattgaa gaaggtaagg atcatatcga ctttcctgac gataaacaac
     9181 gccgtattct ggaaaaactg aaccagacag ttgtatttga acaatttctg gacaagaaat
     9241 acatcggtca gaagcgcttt tctctggaag gcggcgagac taccatcccc gcacttgatg
     9301 ccataatcaa caagggtgct gagctgggtg tggaagaatt cgtgatcggt atggcacacc
     9361 ggggaaggct gaatgtcctg gctaatatca tgggcaagac ctatgaacat attttcaatg
     9421 agtttgaagg aaatgtttcg cctgatctca ctatgggcga tggcgatgta aaatatcatc
     9481 ttggtttttc aagtatggtt accacctcag gcgggcatac cgtgcacctg aagctcagcc
     9541 ctaatccctc acacctggaa gccgtcaacc cggtcatgca gggcttcgtc cgtgcgaagg
     9601 ctgatattat gtatgaggga aaccaccagc gcatcctgcc agtcaccata catggagatg
     9661 ctgctgttgc cgggcagggt gtggtgtatg aagtgctaca aatgagtaac ctgagtggct
     9721 acagagtagg aggtacgata cattttgtca tcaacaacca gatcgggttc actactgatt
     9781 ttgatgacgc cagaacaagc aactactgta cttcgtatgc ttccaccatt caggcaccgg
     9841 tcattcatgt gaacggcgat gatgcagaag ctgtggtcta tgctgtagaa atggctactg
     9901 cctaccgcat gcagtttcag aaggatgttt tcattgacat gttgtgttac cgcaaacacg
     9961 gacacaacga aggtgatgat cctaagtaca cccaaccaca tttatataaa ctgatcgcca
    10021 accatcccaa tccgcgggaa gtttatgtgc agaaactttt gcaggggcat gagatccagc
    10081 aggaaatggc caaatctctg gaaaaagatt tctgggatga actgcaggag cgcctggatt
    10141 atgtaaagca gcataacctg ccatacaaat accaggaacc tgaacaagcc tggagacagc
    10201 ttcgcaaaaa tgttggtcct gaagattttc aatcttctcc aaagactgct atcagcaaaa
    10261 agacacttga tcaggtagca gacggaatat tttctgttcc tgaaggattt aagatactgc
    10321 gtaaggtgca gaagctgttt gacagtagcc agaatatgct caggaacgga caactggatt
    10381 gggcattagg agaattgctg gcctatggat cattgctggc agaaggacat gatgtacggt
    10441 taagcgggca ggatgtaaga agaggcacct ttagtcacag gcattctgta attcgcgatg
    10501 aagaatcaga tgaacttcac aacaggctga atgctattca gaacagaaaa ggtgaattca
    10561 gaatttttaa ctctttgtta tctgaatttg gtgtgctggg ttttgaattt ggatacagta
    10621 tggcctcgcc tgatccactt gtgatctggg aagcgcagtt tggcgatttc agcaatggtg
    10681 cacagaccat tatagaccag ttcatcagtt cttctctgag taagtggcaa agaatgagcg
    10741 gactggtcat gcttctgcca catggctatg aaggccaggg gcctgaacac tcttctgccc
    10801 gtctggagcg cttcctgcag ctctgtgcag agtataatat cgtcgtgtcc aacaccacca
    10861 cacctgccaa ctttttccac ttgcttcgcc ggcaactgaa gtgggatttc cgtcgcccgc
    10921 tcatacatat gagccctaag agcctgcttc gtcacccact ggtcatcagc gatgtgaaag
    10981 acattacctc cggaactttc agagaggtga taggcgatga cttcgctgac gctaagcaag
    11041 tgaagcgtgt tctgctgtgc acaggaaagg tgtattacga tctgttacaa taccagcagg
    11101 agcataaacg aaacgatgtg gctatcgtca ggctggagca attgtatccg ttcccgatga
    11161 cgcaactgga ggagaccctg aaacaatatg gtcatgcacc gatggtatgg gtacaggaag
    11221 aacccatgaa tggcggtgcc tggttcttta ttcacaatat cctggcagac agaggtatca
    11281 cccgtattag cagaaaagca tcagcatcgc cggcatccgg attcatgaaa gtgcacctga
    11341 aggaacaaca gtgcattgtt gaagaggcat tcaaactttg aaagtacaac catgagcata
    11401 caattaaaag taccctccgt gggagagtca gtcaccgagg tcaccctcag taagtggctg
    11461 gtggccgatg gtgattatgt agagatcgac cagagcatct gcgaatttga aagtgataaa
    11521 gccaccctgg aatttcctgc agagaaagca ggacggatct cttttaaagt agaggaaggc
    11581 agtgacctga agatcggtga tgtgatcgct gaactggata cggatgccaa ggcaccggcg
    11641 aagaaagctg ttgagaaaga agttgtttct gcaacgaagg aaactgctaa aactggatca
    11701 aaggaaggca gcgaagaaca aaaaacaaat atcgaaaaac gttcatcgac caacgatact
    11761 tacgcaaaga atcatccttc tcctgctgct cagaagctgg ccgctgaaaa tcatgttcct
    11821 ctggcaaaga tcaacggttc aggcaaggac ggacgcatca ccaaggcaga tgtactgcag
    11881 gctgttaaag atggtgtcag tgcatccggt gcagtggaaa tgatgaagcg aaacagccgt
    11941 gaagaaaagg ctgagaaagt aagcaagctg cgtaagacca ttgccagaag gctggtgagt
    12001 gtaaagaatg aaacagccat gctcaccact ttcaacgaag cagacatgag tgccatcatg
    12061 gagcttcgca cgaagtacaa ggtggcattt gagaaaaagc acggcgtggg tcttggcttc
    12121 atgagcttct ttgttcgtgc ggtaagtaat gcgctggaag aatttcctgc agtgaatgct
    12181 tactggaatg aagaccaggt aatctatcat gattatgttg atatttcaat agcagtgtcc
    12241 actccacgag gactggtcgt gccggtattg cgcaatgcag aaagcatgag tatggcaggc
    12301 atcgaactgg gcatcaagga gctggctgca aaaggcaggg aaggtactct cggtctggaa
    12361 gaaatggaag gaggtacatt taccatcacc aatggtggtg tgttcggttc gctcatgagc
    12421 acacccatca tcaacgcacc gcaaagtgct atactgggaa tgcataagat ccaggaaagg
    12481 ccggtggcaa tgaatggtca ggtggtcatc aggcccatga tgtacctggc attgagttat
    12541 gatcatcgaa tcattgacgg aaaggaatct gtcagcttcc tggtcagggt gaaagagcaa
    12601 ttggaaaatc cgcaacaatt attgtttggt aaagacccgg tagatattct gctggatatg
    12661 tgatcaaata acagcacatg aataagtatg acgttgtagt gatcggctcc gggccgggag
    12721 gatatatagc ggctatccgc tgtgcacagc tgggtatgaa gacagccatc attgagaagt
    12781 atgatacgct gggaggtacc tgcctgaatg tgggatgcat tcccagtaaa gcattgcttg
    12841 actccagcga acattatcat aatgctgccc acagttttga gaaacatggc atcggtgtga
    12901 gcggattgtc tgtagatttt tctgccatga tagcacgcaa ggcaggtgtg gtcaaacaga
    12961 ccacagatgg catcaacttc ctgatgaaga aaaataagat cgatgtctat cacggacatg
    13021 gatcctttga aagcaggaac accattgcca tagccaaaac ggatggtaaa acagacaaga
    13081 tagaagcaga caaggttatc attgcaaccg gctccaagcc tgcatcatta cccttcatca
    13141 ccattgataa aaaacgtatt atcaccagta cagaggcttt gtcactggac aaacttccca
    13201 aaagcatgat cgtactgggc ggtggggtaa taggctgcga gctgggttct gtatatgcac
    13261 gcctgggaac agaggtcacc atcgtagaat atatggacaa cctcattcct accatggacg
    13321 cagatctggg taaagacctg cagcgctccc tgaagaagat gggtatgaag atgcagctca
    13381 gtactaaagt gacggcagta aaagctacca ccaaacaggt caccctcact gctgagaaca
    13441 agaaaggaga gtctatagaa atgaaggccg attattgcct ggtctccgtg ggtcgcaaac
    13501 cctatacaga taaactgggc ctggataatg tgggcattga actgaatgaa cgcggacaga
    13561 taccggtcaa tgaaaaactg gaaaccaaag tggcgggaat ctacgccatc ggcgatgtga
    13621 tacaaggtgc catgctggct cacaaagcgg aggaagaagg cgtgttcgtg gccgagacca
    13681 tggcaggaca acatccgcac gtcaactatc tgctcattcc gggagtggtc tatacatggc
    13741 cggaagttgc aagtgtaggg tacaccgaag cacaactgaa agaaaaaggc accgcctata
    13801 agacaggtaa gtttccattc atggccagtg gtcgtgcaag ggcaagcatg gatactgacg
    13861 gttttgtgaa agtactggca gatgccggaa ccgatgagat actcggagtg catatgatcg
    13921 gtccgcgggc agcagacatg atcgcagaag cagtggtggc catggagtac cgtgccagtg
    13981 ctgaagacat cggcagaatg agccatgcac accctactta cacagaggcg ttcaaggacg
    14041 cttgtctggc tgcttcagaa aaccgagctt tgaacatctg acccatgaaa aaatacatct
    14101 tccctatgct catcgccgga ctggcgttct cttgtcaggc gcaggatgac aaacgtgccg
    14161 gaattctttt ctacaacgtg gaaaatctga tggataccat agacaatccc gtaaagatcg
    14221 atgatgagtt tgtgcccaca tcagagaaac agtggaacac agaacgctac caaaccaaac
    14281 tggatcacat agggcaggtc attgccagta caggagtgca gttgccggtc attgtaggac
    14341 tggctgaaat tgagaaccgt ttcgtcctgg aagatctggt aaaggaagca catatgcagg
    14401 ccggacatta tggtatcgtg cagttcgatt cacctgatga aagagggatc gattgtgcgt
    14461 tgctgtatga tcagcagcgc tttaaagtgt tggatgccta tcccgttcct gtttatctgc
    14521 caaaagataa tggaggaagc acccgcgaca ttctctatgt gacaggtaag ttcaatggaa
    14581 cagatgaggt ggtgcatatt tttgtcaacc actggccatc caggtatgaa ggagaagagg
    14641 tttcccgtcc gcgacgcatc atggccgcac aggtactacg ccaccaggta gatagcctca
    14701 acgatctgta cagcgatccg gctatcatca tcatgggcga tttcaacgat acacctttcg
    14761 atgaaagtgt catggtaacc ctcggtgctg taactcctga cgggcactat acagatgaaa
    14821 gcctggtcaa cctggtagcc aatttccaga aagataatgt gggctcgtac aattataaag
    14881 gaaactggca ggcgcttgac cagcttatag tgagtgaatc catattaact gacgatgccg
    14941 ctttccgggt agatgaccgt aaggtccgtt tcattcgcaa cgactttcag atgtaccaca
    15001 gtgataagta tg
//
LOCUS       DAOMAX010000042        14945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_467_length_14945_cov_5.083747, whole genome shotgun sequence.
ACCESSION   DAOMAX010000042 DAOMAX010000000
VERSION     DAOMAX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_467_length_14945_cov_5.083747"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..602)
                     /locus_tag="PK742_03800"
     CDS             complement(138..602)
                     /locus_tag="PK742_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HQU75743.1"
                     /translation="MSRVRFIWIAGLMLGLAACEQNRIFEQNLPVDVNGWSYTDPKEY
                     TIVIQDTSILYNVFVNVRHADTYRYNNLWVILQTTMPDGSALSDKIQIALALPEGSWT
                     GTCIDGICYHSVLIRSRVTFPQSGSYTLRLVQDMREEPLPGLLDVGIRIERL"
     gene            complement(599..1888)
                     /gene="ricT"
                     /locus_tag="PK742_03805"
     CDS             complement(599..1888)
                     /gene="ricT"
                     /locus_tag="PK742_03805"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQU75744.1"
                     /translation="MSCAGCSVGTSGVPAGCGSNGHCLTGGCNKLNTHNWLAHLPAPP
                     ELRFQIYEVSFKNGLHKSFFANPKGLDLMTGDLVVVDADPGFDVGRISLSGELVRLQM
                     KRRKVSENSKLPAIQRLAGEGDKERLTESRAKEGATMIKARTIARELKLDMKVGEVEF
                     QGDGRKATFYYIADGRVDFRELIKIYAREFKVKIEMRQIGARQEAGKIGGIGSCGREL
                     CCSTWLTDFKSVSTGAARYQNLSINQAKLSGQCGRLKCCLNYELDTYLDALEAFPKDM
                     NVIQTKNGKARLSKTDIFRRTMVYDMEGSASPVTITLDKIAQIRTRLQNGEELEGLDE
                     FKEVEVVQVVEKFADVTGEVTLKTLEKAGNKKRQKNRNKNRGNNERQKTDNKESKKQT
                     GPRNEGGNKQENRRSSRNRNRNRNKGNNRNTGGDQNS"
     gene            complement(1901..3073)
                     /locus_tag="PK742_03810"
     CDS             complement(1901..3073)
                     /locus_tag="PK742_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75745.1"
                     /translation="MLFREVIGHEALKHRLISSVQQNRVPHAQLFLGPEGCGNLPMAL
                     AFAQYLNCESPGNTDSCGQCPSCQKALKNIHPDIHFTFPFIAISTGKKEEIKEKCADW
                     LPEWRTFLQQQPYGNYNNWINFIQKENKQGNINAKECGDIIHRLSFKTFESSWKVLVL
                     WLPEFLEKEGNRLLKLIEEPPEQTIFLFVGHNPDRIINTILSRLQIVPFGRLQEDEVA
                     WYLQEEQGVPAQSARQVAVLSNGNIARAAELVQVSQESYTSTLRQWMLACHAYDITDM
                     FAWNEQFIKWGREQQKQFFFYCIHFFREVYLMQHGAAVLNRLTEDEMNFAQKLGGKLS
                     AASVERIMELFDTYIYFIERNASARILITQMTVSFAHFFHRHPADIRHPFYAEWEL"
     gene            3150..3557
                     /locus_tag="PK742_03815"
     CDS             3150..3557
                     /locus_tag="PK742_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQU75746.1"
                     /translation="MRTAVIRRVTFNAAHRLFRADWSEERNREVFGLCSNPNYHGHNY
                     TLFVRVTGDIDPETGYVLDLKVLKDIISEEVTGPFDHSNLNLDRPEFANLNPTAENIA
                     RVAWQKIRKRLPDHMDLQIRLSETDNNFVEYPA"
     gene            complement(3554..3943)
                     /locus_tag="PK742_03820"
     CDS             complement(3554..3943)
                     /locus_tag="PK742_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQU75747.1"
                     /translation="MEHIIQVRSVTGETPYRTEIQAGTHIILADEPEESGGQDLGATP
                     TRYLCSALASCIGITLRMYADRKEWKTGAITVDVELDRSGDKPVFSIGLRFANTLPED
                     QLQRLQLIAGKCPVHKLLHGNNEFRYV"
     gene            4095..5093
                     /locus_tag="PK742_03825"
     CDS             4095..5093
                     /locus_tag="PK742_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQU75748.1"
                     /translation="MEQLASDIQELHERIHEESAFIDLLRLETSKAIVGQKYMIDRLL
                     LGLLAQGHILLEGVPGLAKTLAIKTLAASVDARFSRLQFTPDLLPADLVGTLIYNQKE
                     AAFEVRKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDKTYPLKEPFLVLAT
                     QNPIEQEGTYPLPEAQVDRFMLKVVISYPSKEDEQQIIRQEVGGKRPVVAQVVKPDAI
                     LKARDSVRQVYMDEKIERYILDIVFASRTPADYQLDKLKHLIAYGGSPRASINLALAS
                     KAYAFIKRRGFVIPEDVRAVCHDVMRHRIGLTYEAEAENITSEEIIDEILNVVEVP"
     gene            5174..6049
                     /locus_tag="PK742_03830"
     CDS             5174..6049
                     /locus_tag="PK742_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQU75749.1"
                     /translation="MDKATLLKKVRLIEIKTRGLSTQLFSGEYHSAFKGRGMSFSEVR
                     EYTYGDDVRNIDWNVSARMHHPYIKVFEEERELTVMLLIDISRSAYYGTHQQTKNELI
                     TEISAVLSFSAINNNDKVGVILFSDRIEKFIPPKKGKSHILRIISDLLETKPDGTGTD
                     IALLLEYFSNVVRKRSIAFLFSDFMDNDYLRPLRIAAKKHDLIGVRLFDPTEAQLPDA
                     GLVQMQDSETGELIWVDTSSAEVRKQYHRNYLQRVAGFEANFARCGADTIAIATNQPY
                     MPALHGFFRKRMQRR"
     gene            6046..6945
                     /locus_tag="PK742_03835"
     CDS             6046..6945
                     /locus_tag="PK742_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75750.1"
                     /translation="MKRLLVISLLFTAMVSFAQQVTVSTDTTTYLIGDYIRVQLQADA
                     PAGTTITFPQADLMDPELEVISVNPPDSIPGSTGIRQEMVFSVYDSGTYRVPQLPFVF
                     TSKNSTDTIYSDSLLFRVNTLEVDTTAAIKPIKDNLEVSYRDLRWLYYAGGGLLLLVI
                     AAATIWYLRKRKQQKAAAPVILRTKLSTYTLEQLQQLDAEQLWQQGQIKSYYSGLTDI
                     LRRYIEQRFGFPAMERTSDEILELLRGAQTAAPDEIFRLADMAKFAKSRPLPDQNKRA
                     MELAVQWVTETTPDNEPEMDLPG"
     gene            6942..7949
                     /locus_tag="PK742_03840"
     CDS             6942..7949
                     /locus_tag="PK742_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQU75751.1"
                     /translation="MTEWISDIAFADPWYFLLLLVIPLYLFLRKKWLRRTRVPFMIST
                     GAGVHTGTWRTRFIWIPEALRMLAIASAVIALARPQTGFSNRNVTTEGIDIVMALDIS
                     SSMYAIDFQPNRITAAKEAAKEFIDNRPNDRIGLVVFAGETFTQCPITLDHLLLKTLV
                     DDADNWQLEDGTAMGDGLFMAVNRLADTTQLNTKVVILLTDGVRTAGEFSPLDAANAA
                     AQLNIRVYTIGVGSKSNAPIPVVDKNGRNIYDLDPRISFDEESLQQIADITGGQYFRA
                     TSKEKLKEVYREIDSIEKQKVEVSVTRRYDERFHIFALIAIIALMFELLLRFTIFKSL
                     N"
     gene            7949..8992
                     /locus_tag="PK742_03845"
     CDS             7949..8992
                     /locus_tag="PK742_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQU75752.1"
                     /translation="MQWEHVTYLYGLLVIPVLFMLFLLALRIRSRQMRRWGDQLLVQR
                     MMEHFSLSRRWWKTILSLLALACFILALANLRMGSKREKVQAQSSEVIICFDVSRSMQ
                     AEDVKPDRLSRARILASQIIESLSGNKVGLIVFAGKAYVQMPLTVDSRAALMYLNTVN
                     TDMVQTQGTAIGEALTAAKLVFDNGGESSGNNRAVILITDGESHDEDAPNAASELADM
                     GVHIITIGVGTEKGAPIPMERNGGSDFKKDNQGNIVLSKLNESMLEELSNTGNGFYMN
                     LSQGRAVVQQVEKEVSGLDKDSGQEYTFVEYRNHFQVFLLAGILLLLLEMLISEKGNK
                     IIRRLLRTETTGQ"
     gene            8989..9714
                     /locus_tag="PK742_03850"
     CDS             8989..9714
                     /locus_tag="PK742_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU75753.1"
                     /translation="MKGNFLIILMLLSPGVFAQQENKYIQEGNKQYDRKQYDAAEQYY
                     RQALEKQPARQEATYNLGNALYNQQKLDESIASYDAIASTTSDPSIRSKAYHNMGNAY
                     LEKKDYEKSIEAYKQALKADPTDMDTKYNLAYARSMLKQQQDQQQQNKQDQQDKQDQQ
                     QNQDQQNQDQNKQDQQNQNQNKQDQQQQNQQQENRQPQDKKFTPEELERIMQTLNKDD
                     KDVQNKVSKQRTEGSRTKSDKDW"
     gene            9763..11481
                     /locus_tag="PK742_03855"
     CDS             9763..11481
                     /locus_tag="PK742_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQU75754.1"
                     /translation="MATAQTTFDAQVSRTQASTADRIELSFTLTNGNKPKNFKAPVMN
                     GLRVLGGPYQSSSFQSINGRTSSSISYKYVVQGSKSGTVTIGSATVEAGGKTYSTKPI
                     TLTFTDAPVQADQSDSNGDLQAFLRNNFYLRAVVSDNDVYAGEELTVTYYIYINARSS
                     ITDYRLNGATQVPAFDGFYAEDIDVSHVQGEYQTINGQQFLVQPVKKVRLTPQRAGDL
                     LADPMTVDATLSIRKQRSNDSFMDDFFGRGYDRINASATSPSVRIRVRDLPQPAPASF
                     NGAVGSFDMHTTINTTKTTTDEPITYKVEVKGKGNLELFQVPELDLPPGWETYEPEVT
                     STASSKTYEYLLIPRSAGNFELPPHEWSYFDPKERQYFTLESEGYSIEVEQGKQYSGA
                     GTTKEDVEELNRDIRFIGRSEPHFSKGSGIRVGSPVFATAVGFPFAAGLFTWLFLFWR
                     NRHDDPVARAHKRAGSKALKQLRAAKELSEGDDTKAFCNEAIRVIWQYLGSKWQLPVS
                     RQNKEQITQIFAEKDAPDDLRDETIELLRKLERSIYAPAISGSSSRELFEELESWITH
                     MEKWQA"
     gene            11478..12224
                     /locus_tag="PK742_03860"
     CDS             11478..12224
                     /locus_tag="PK742_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU75755.1"
                     /translation="MRKWILSIVVLTTLQATAQDSLWQAANIAYEQGFYKNADSLYTI
                     LLNDHGPSAEVYYNLGNTCYKQEDWPRAILNYERCLQIDRRHKDAIFNLELSNTHLRD
                     RIDPIEPSLLHLFWRDLCHLLPSGTWGWITLFFAWSSLGFLLLLLFSKNLSLRKTGLY
                     GMFSAAALTILLLAITYAAHYQEEREQYAIIMQPSLILKSEPSESSTNLFVLHSGLKL
                     RILDSDDSWSLVSMPDGNTGWVQTEALEQI"
     gene            12558..13262
                     /locus_tag="PK742_03865"
     CDS             12558..13262
                     /locus_tag="PK742_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQU75756.1"
                     /translation="MTTKTAFNPFEHSRLFFMAGVAISLGIIQLAFGWNGMHRIPQLP
                     DLPKISDGTWVEVEYFTEKVEEQLPEKQVQKEEVIIPEPDAPIKVVDDVKKITTPIKL
                     NNPEKDPVGLKGPSRTATTTTGGGTEKKEIGTIAEVMPAFPGGYEALYKWLAKNTSYP
                     KWAKDAGIEGTVYVEFILGTDGKVQSAKVLRGIGGGCDEEVLNAVLRMPAWNPAKQGN
                     VEVPLRMTIPFTFKIS"
     gene            13556..14260
                     /locus_tag="PK742_03870"
     CDS             13556..14260
                     /locus_tag="PK742_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQU75757.1"
                     /translation="MTSPTAFDPFSHTRLFFMTGLAVALLFILAAFRWDVSLPQRPTP
                     DQETAPDFGDPITLIAPVVVPLPEHPLVMEAPKTPPMIDLSADILEVPDEIPLTARLN
                     FTEPDDTPVPLKGFTGNTVLPEMPSETDDARLAAEVMPEFPGGKEALYKWLSTNIKMP
                     SVAIDLNMSGVVYVDFVIDTNGKVTRVKIRKGTGTVLDEAALEAIQRMPDWTPGMQGG
                     RPVPVFQTLPVRFELR"
     gene            complement(14380..>14945)
                     /locus_tag="PK742_03875"
     CDS             complement(14380..>14945)
                     /locus_tag="PK742_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75758.1"
                     /translation="MPLLGTFYLLYSNPFSYPDPGENFMLLLRVALLTFFFPAVFVGL
                     MSALKFIGSISLRDRQDRVVPYIATMAFYIWAFYVFYSESFNGMVTFLLLASCISIVL
                     AFLINILFLKVSMHTTAAGGMVAFFLLLIPFSQYNALWPFLTIILIAGLVGTSRLILS
                     AHSKKEIYLGYLVGFMSFMIAMNIFHF"
BASE COUNT         3984 a         3772 c         3664 g         3525 t
ORIGIN      
        1 tacaaagaag catagtcgtt caggtcatcc agcagaataa cgataacgtt gggctgttcc
       61 tgtgccggca gcaaaaccgg ataacaatgc aaagcagtca acaggagtcc ggttcggaac
      121 agtgcgcctg acatacctta taatctttct atccggatgc caacatccag taaccccggc
      181 agaggttctt cacgcatatc ctgtaccagg cgcagggtat aactgccgga ttgaggaaat
      241 gttacacggc ttctgatcag tactgaatgg tagcagatac catcaataca ggtaccggtc
      301 caggaacctt caggcaatgc cagtgctatc tgaatcttat cgctgagtgc agaaccatca
      361 ggcatggtgg tttgcaggat cacccatagg ttattgtacc gataggtatc tgcatgtctt
      421 acattcacaa acacattata aaggatcgag gtatcctgaa tgacgatggt gtattctttg
      481 gggtctgtat aagaccagcc atttacatct accggcagat tctgctcaaa gatgcggttc
      541 tgttcacagg cggccagccc cagcatcagc ccagcaatcc atatgaacct gaccctgctc
      601 atgaattctg atcgcctcct gtattcctgt tgttgccttt gtttctgttt cggttcctgt
      661 tgcgggatga acgcctgttc tcctgcttgt tgccaccttc gtttctgggt ccggtctgtt
      721 ttttactttc cttattgtcc gttttctgtc gttcgttgtt gccgcgattt ttattgcggt
      781 tcttctggcg ctttttatta ccggcttttt ccagcgtttt caaagtaacc tctccggtca
      841 catctgcaaa cttttctacc acctgcacca cttccacttc tttgaactca tccagccctt
      901 ccagttcttc accgttttgt aaacgtgttc tgatctgtgc aattttatca agggtgatgg
      961 taaccgggct ggcgctgcct tccatatcgt acaccatggt acggcggaaa atatctgtct
     1021 tgctgagtct tgcctttccg ttcttggtct gaatgacatt catgtctttc gggaaggcct
     1081 ccagggcatc caggtaggtg tccagctcat aattgagaca acatttgagc cgcccgcact
     1141 gaccactgag ttttgcctgg ttgatgctga ggttctggta acgtgctgcg ccggtactga
     1201 ccgatttgaa gtcagtcaac caggtggagc aacacagctc tcttccacag gaacctatgc
     1261 ctcctatctt acctgcttcc tgtctggcac cgatctgccg catttcaatt ttcaccttga
     1321 attctcttgc gtagatcttg atcagttcgc ggaaatcaac tcgaccatcg gctatatagt
     1381 aaaaggtggc ttttctgccg tctccctgaa actccacctc accaactttc atatccagtt
     1441 tcagttcacg tgcaatggtg cgggccttga tcatggtagc cccttccttt gcacggctct
     1501 cggtaagtcg ttccttatcg ccttctccgg ccagtcgctg aatggcagga agcttgctgt
     1561 tttcagaaac ctttctgcgt ttcatttgca accgcaccag ttctccggat aatgatattc
     1621 gtccaacgtc gaaaccaggg tcggcatcaa ccaccaccag gtcgccggtc atcaggtcaa
     1681 gtcctttcgg gttggcaaag aagctcttat gaagtccgtt cttaaatgat acttcataga
     1741 tctggaaacg cagctcgggg ggggcaggca aatgtgcaag ccagttgtgg gtgttgagtt
     1801 tattgcaccc gccggtcaga caatgtccgt tactgccgca acccgccggc acaccactgg
     1861 ttcctacact acatcctgca caactcatat gtttacaaag ttacaattcc cattccgcat
     1921 agaaaggatg ccgtatatct gccggatggc gatggaagaa atgagcaaag gaaacggtca
     1981 tctgggtgat gagaatgcgg gcgctggcat tccgttcaat gaagtagata taggtgtcaa
     2041 acagctccat gatgcgttcc acactggcgg cagaaagctt gcctccaagc ttttgggcga
     2101 agttcatttc atcttctgta agccggttaa gaaccgcagc tccgtgttgc atcagatata
     2161 cctcccgaaa gaaatggatg cagtaaaaaa agaattgttt ttgttgttca cgcccccact
     2221 taatgaactg ctcattccag gcaaacatat ctgttatatc atacgcatgg caagccagca
     2281 tccattgccg cagggtggag gtgtatgatt cctggcttac ctgaaccagc tctgcagctc
     2341 gtgcaatatt gccattgctg agaacagcta cctggcgggc actttgggca ggcacaccct
     2401 gttcttcctg caggtaccac gccacctcgt cttcctgcaa ccgaccgaag ggcacgatct
     2461 gcaaacggct taagatggtg ttgatgatgc gatcaggatt gtgccccaca aacagaaata
     2521 ttgtttgctc aggaggttct tcaatcagct tgagcaggcg gttgccttcc ttctccagaa
     2581 attcaggcaa ccagagtacc agcaccttcc aggacgattc aaaggttttg aatgacaggc
     2641 ggtgaatgat atcaccgcat tctttggcat tgatatttcc ctgcttgttc tccttctgga
     2701 taaagttgat ccagttattg taattgccat agggttgctg ttgcagaaat gtacgccact
     2761 cagggagcca gtctgcgcac ttctctttga tctcttcttt cttacctgta gatatggcaa
     2821 tgaaagggaa tgtgaagtgt atatccggat ggatattttt caatgctttc tgacaggacg
     2881 gacattgccc gcaggaatcg gtattgcccg gagactcaca gttcagatac tgggcgaagg
     2941 caagtgccat gggcaggttg ccacatcctt ccggacccag aaacagctgg gcatgcggta
     3001 ccctgttttg ctgcacactg ctgataaggc ggtgtttgag agcctcatga cctatcactt
     3061 cccggaataa catgggcgac taagttacac caacagcctt tcctgatgta attttgaacg
     3121 aagcatccag cttggatgtt cttgtttcta tgagaacagc agttatcaga cgggtcactt
     3181 ttaatgcggc acaccgcctg ttccgggcag actggagcga agagcgcaac cgcgaggttt
     3241 tcggtttgtg cagcaatccc aactaccacg ggcacaatta caccctgttt gtcagggtta
     3301 ccggcgatat tgatccggaa acaggctatg tattagacct taaggtgctg aaagacatta
     3361 tcagcgaaga agtgaccggc ccgtttgatc acagcaacct caacctggac aggccggaat
     3421 ttgcaaacct gaatccaacg gctgagaata ttgcccgggt ggcctggcaa aagatccgca
     3481 aaagactgcc agaccacatg gatctgcaga tacgcctgtc agaaactgat aataacttcg
     3541 tggagtaccc ggcttagacg tatctgaatt cattgttgcc gtgcaataac ttatgcaccg
     3601 ggcattttcc ggctatgagt tgtaagcgct gcagctggtc ttccggcagg gtgttcgcaa
     3661 agcgtaaacc gatgctgaat actggtttat cgccacttct gtccagttct acatctacgg
     3721 tgatggcacc ggtcttccat tcttttctgt cggcatacat ccgcagggtg atcccgatac
     3781 aggatgccag ggcgctgcac aggtaacggg ttggcgttgc gcccagatcc tgcccgccgc
     3841 tttcttccgg ctcatctgca agaataatat gggtgccggc ctggatctct gtgcgatagg
     3901 gcgtttcgcc tgtcaccgac cgtacttgta tgatatgttc cacagtgtgg ttttgattaa
     3961 ttatgccaaa agtaggaggt ctgttaacaa caattaaccc acaagaatca tcttttcact
     4021 tgacatgccc gttttcaatg ctataaattt gtcacccatt ttggttctta cacataaaac
     4081 agggaagttg cgatatggaa caactcgcat ctgatattca ggagctgcat gagcgcatac
     4141 atgaagagag tgcctttatt gatctgctcc gcttagagac ctccaaggcc attgttggcc
     4201 agaaatacat gattgaccga ttgctgctgg gactgctggc ccagggacat atcctcctcg
     4261 agggggttcc cggactggcc aagaccctgg ccatcaaaac ccttgcagcc agtgtggatg
     4321 cacgcttctc gcgattgcag ttcacgcctg acctgctgcc cgccgatctt gtaggtaccc
     4381 tgatctacaa ccagaaggaa gcagcttttg aggttcgcaa gggccccatc tttgccaatt
     4441 tcattctggc tgatgagatc aaccgggcac ccgccaaagt acaaagcgct ctgctggaag
     4501 ctatgcagga aagacaggtg accattggcg ataagaccta tccgctcaag gaaccctttc
     4561 tggtactggc aacccaaaac cccattgaac aggagggaac ctacccgctg cctgaagcac
     4621 aggttgaccg cttcatgctg aaagtggtca tttcctatcc aagcaaagag gatgaacaac
     4681 agatcatccg gcaggaagtt ggtggcaaaa gaccggtggt ggcccaggtt gtaaaaccag
     4741 acgccatact gaaggcaaga gatagtgtgc gtcaggtgta tatggacgaa aagatcgaac
     4801 gctatatcct ggacatcgta tttgcatcac gcactccggc cgattatcag ctggataaac
     4861 tcaaacacct catcgcctat ggcggttctc cacgtgcatc tatcaacctg gcactggcaa
     4921 gcaaggcata tgcttttatc aaacgaaggg gctttgtaat tccggaagat gtacgtgcag
     4981 tttgtcacga tgtgatgaga caccgcatcg gactgaccta cgaagcggaa gccgagaaca
     5041 tcacatctga agagatcatt gacgagatac tgaatgtggt tgaagtacct tgagatcgtg
     5101 gtaccgaaat agcgtgaaaa gcccggcatg acacctgttg gtttattcgc aatgaaaacc
     5161 tgctgcatga ccaatggata aagctacact acttaaaaaa gtccgcctga tcgagatcaa
     5221 gacccgaggt ctctcgaccc agctgttttc cggagagtac cacagtgctt tcaaaggcag
     5281 gggaatgtcg ttcagtgagg tgcgtgaata tacctatggt gatgacgtgc gcaacatcga
     5341 ctggaatgtg agtgcccgaa tgcaccatcc ctatatcaaa gtattcgaag aagaaagaga
     5401 gctgaccgtc atgttgctca ttgacatcag ccgttcagca tattatggta cccaccagca
     5461 gaccaagaat gaactgatca cggaaatttc tgccgtgttg tctttctcag ccatcaacaa
     5521 caatgataaa gtgggggtca tcctcttttc tgacagaatt gaaaaattca ttccgcccaa
     5581 gaaaggcaag tcgcatattc tgagaatcat cagcgatctg ctggaaacca aaccggatgg
     5641 aacaggaaca gatattgccc tactgctgga atatttcagc aacgtggtac gcaaacgaag
     5701 catcgctttt ctgttctctg atttcatgga caatgattat ctgaggcccc tgcgcattgc
     5761 agcaaaaaaa cacgatctga taggagtaag gttatttgat cctaccgaag cgcaactgcc
     5821 tgatgccgga ctggtgcaga tgcaggatag tgaaacaggc gaactcatct gggtggatac
     5881 atcatcggcc gaagtgagaa aacaatacca ccgcaattac ctgcaaaggg tagcaggatt
     5941 cgaggccaac tttgcacgtt gcggggcaga taccatcgcc atcgccacca atcagcccta
     6001 tatgcccgcc ctgcatggtt tcttccggaa aagaatgcaa cgcagatgaa aagactgctg
     6061 gtcatatcac ttctgtttac tgctatggtt tccttcgcac agcaggttac ggttagcaca
     6121 gatacgacca cctatcttat tggcgattac atacgggtgc agttgcaggc cgatgcaccg
     6181 gcaggtacca ccatcacctt cccgcaggcc gatctgatgg accctgaact cgaggtgatc
     6241 agtgtcaatc cgcctgacag tatcccggga agcaccggca ttcggcagga aatggtattc
     6301 agtgtgtacg atagtggcac ctaccgcgtc ccgcagcttc ccttcgtttt tacttcgaag
     6361 aacagcacag acaccatcta ttcagacagc ctgttgttcc gtgtcaacac attggaagtg
     6421 gatactacag cggccatcaa accgatcaag gataatctgg aagtttctta ccgcgatctt
     6481 cggtggttgt attatgcagg tggcggactg ttattgcttg ttattgcagc ggccaccatc
     6541 tggtatctgc gcaaacgcaa acagcaaaaa gcagcagctc cggtcattct gcgtactaaa
     6601 ctgtcaacct atacactgga acaattgcag caactggatg cagaacaact ctggcaacaa
     6661 ggacagatca agagctatta ttccggattg acagatattc tgcgacgtta catagaacaa
     6721 cgatttggtt tccctgccat ggagcgaacc agcgatgaga tcctggaatt gcttcggggt
     6781 gctcagaccg cagcaccgga tgagatcttc cgcctggctg atatggctaa gtttgccaaa
     6841 tccagaccac tgcctgacca gaacaaacgg gccatggaac tggcagtaca atgggtcacc
     6901 gagaccacgc ctgataatga acctgaaatg gacctgcccg gatgacagaa tggatcagtg
     6961 atatcgcctt cgccgacccc tggtacttcc tgctgctgct ggtcattccg ctctatctgt
     7021 tcctgcggaa aaaatggctc agacgcacca gggttccttt catgatctcc accggagcgg
     7081 gggtacatac aggcacatgg agaacacgct tcatctggat acccgaagcc ctgcgcatgc
     7141 tggccattgc atctgcggtc atcgcactgg ccagaccgca aacaggtttc tccaacagaa
     7201 atgtcactac agaaggaata gacatcgtga tggcactgga tatttccagc agtatgtatg
     7261 ccatagactt tcaacccaac cgcatcactg cagcaaaaga ggccgccaaa gaattcatag
     7321 ataatcgtcc gaacgatcgc atcgggctcg tggtgtttgc cggagagacc tttacacaat
     7381 gtcccatcac cctggatcat ctgttgctga agacgctggt agatgatgca gacaactggc
     7441 aactggaaga cggaacggct atgggcgacg gactcttcat ggcagtgaac cgtctggccg
     7501 ataccaccca gctcaatacc aaggtggtca tcctgcttac tgatggggtg cgcaccgccg
     7561 gcgagttctc tccgctggat gctgccaatg cggcggcaca gctgaacatc cgggtctata
     7621 ccattggcgt gggaagcaaa agcaatgcac ctattccggt ggttgacaag aacggacgga
     7681 acatctatga tctcgatccg cggatctcat ttgatgaaga atcactgcag cagatagcag
     7741 acattaccgg cggacagtat ttccgtgcta cttccaaaga gaaactaaag gaagtgtacc
     7801 gcgagataga cagcatagaa aaacaaaaag tagaagtgag cgtgaccaga cggtacgatg
     7861 aacgcttcca tattttcgct ttgatagcca tcattgcact catgtttgaa ttactgttac
     7921 gtttcaccat tttcaaaagc ttgaactgat gcagtgggaa catgtaacat atctctatgg
     7981 tttgctggtg ataccggtgc tattcatgct ctttctgctg gcacttcgca ttcgttccag
     8041 acaaatgcga agatggggag atcaactact tgttcaacga atgatggagc acttctcact
     8101 ctcgaggcgt tggtggaaga ccattctttc tttgctggcg ctggcctgtt tcatcctggc
     8161 actcgcaaac ctcaggatgg gcagcaagcg ggaaaaagtg caggcgcaaa gcagtgaggt
     8221 catcatttgt tttgatgtgt cacgcagtat gcaggcagaa gatgtaaaac ctgatcgctt
     8281 gtcaagagcc cgcatcctgg cttcgcagat catagaatca ctgtcgggca acaaggttgg
     8341 cctcattgta tttgccggca aagcttatgt gcagatgccg ctcacggttg acagccgggc
     8401 agctctgatg tatctcaata ccgtcaacac cgatatggtt cagacccagg gtacggccat
     8461 tggtgaagca ctgactgctg ctaagctggt gttcgataat ggtggtgaaa gcagtggcaa
     8521 caaccgggcg gtcatcctga taaccgatgg cgaaagtcac gatgaagatg cgccgaatgc
     8581 agcttcagaa ctggcagata tgggcgtgca catcatcact atcggagtgg gcacagagaa
     8641 aggagcacct attcctatgg agcgaaacgg aggcagcgat tttaaaaaag ataatcaggg
     8701 caacatcgtg ctcagcaaac tgaatgagtc catgctggaa gaactaagca ataccggcaa
     8761 cggattctat atgaacctca gccagggtcg tgccgttgta caacaggtgg agaaggaagt
     8821 gtcgggactg gataaggaca gcggacagga atatactttt gtggaatacc gcaaccactt
     8881 tcaggtattt cttctggctg ggatcctgtt gctgttgctg gagatgctca tcagcgaaaa
     8941 aggaaataag atcatacgca ggttactgag aacagaaacc acaggccaat gaaagggaat
     9001 tttctgatca ttttaatgct gttgtcgccg ggtgtatttg cccagcagga gaacaagtat
     9061 atccaggaag gtaacaagca atacgaccgg aagcagtatg atgcagcaga acaatattat
     9121 cgtcaggcac tggaaaaaca acccgcccgt caggaggcta cctacaacct gggcaatgcg
     9181 ctgtataacc agcagaaact ggacgagagt attgccagct atgatgccat cgcttccacc
     9241 accagcgacc catccatccg ctccaaagcc taccacaaca tgggtaatgc ttacctggag
     9301 aagaaggatt atgagaaaag cattgaagcg tacaaacagg cgctgaaagc cgatcccaca
     9361 gacatggaca ccaagtataa tctggcctac gcccggtcca tgctcaaaca acaacaggat
     9421 cagcagcaac aaaacaagca ggaccagcag gataagcaag accagcagca aaatcaagat
     9481 caacagaatc aggaccagaa caaacaggat cagcagaacc aaaatcagaa taagcaggat
     9541 cagcagcagc aaaaccagca gcaggagaat cgtcagccac aggacaagaa attcactcct
     9601 gaagaactgg aacgtatcat gcaaaccctg aacaaagacg acaaggacgt gcagaacaaa
     9661 gtgagcaagc aacgaaccga agggtccaga acaaaatcag ataaagactg gtaattgaac
     9721 aggatacaac atatattatt cattttatta tttattggta gcgtggcaac tgcccagacc
     9781 acgtttgacg ctcaggtgtc acgtacgcag gccagcaccg cagaccgtat agaactcagc
     9841 ttcaccctta ccaacggcaa caagccgaag aacttcaagg ctcctgtcat gaatggattg
     9901 cgggtgctgg gtggtcctta ccaaagcagc agttttcaat ccattaatgg tcgcacctcc
     9961 agcagcatct cctataaata tgtggtgcag ggaagcaagt caggaaccgt gaccattggt
    10021 agtgccacag tggaggcagg tggaaagact tatagtacca aacccattac gctgactttc
    10081 actgatgcac ccgttcaggc agaccagagc gacagcaacg gagacctgca ggctttttta
    10141 cgaaataact tttacctccg ggcagtagtg agtgacaatg atgtgtatgc cggtgaagaa
    10201 ctgacggtca cttattatat atatatcaat gccaggtcat ctattaccga ctaccgcctg
    10261 aatggagcta ctcaggttcc ggctttcgac ggtttttatg cagaagatat tgacgtcagt
    10321 catgtgcagg gtgaatacca gaccatcaac gggcagcagt tcctggtgca accggtcaag
    10381 aaggtcagac taacacccca gcgggccgga gatcttcttg ccgatccgat gaccgtagat
    10441 gccacactga gcatccgcaa acaacgcagc aacgattctt ttatggatga cttctttggc
    10501 agaggatacg accgtatcaa tgcttcagct acgtcgccat ccgtacgtat tcgggtgaga
    10561 gatctgcccc aacctgcacc ggcatcattc aacggagctg ttggcagttt tgacatgcat
    10621 accaccatca acaccacgaa gaccacaaca gacgaaccca tcacctataa agtagaagta
    10681 aaaggcaagg ggaatctgga actgtttcag gttcctgaac tcgatctgcc tcccggatgg
    10741 gaaacgtacg aaccggaggt cacttccact gccagcagca agacctacga atacctgctc
    10801 attccgcgaa gcgccggcaa ctttgaactg ccgccgcatg agtggtcgta ttttgatccg
    10861 aaagaaaggc agtatttcac cctggaatct gaaggataca gtattgaagt ggaacaaggc
    10921 aaacagtaca gtggagcagg taccaccaag gaggatgtgg aggaactcaa ccgcgatatc
    10981 cgcttcatcg gccggagtga gcctcatttc agtaaaggca gtggcatacg tgttggaagc
    11041 ccggtgtttg ccacagccgt tggatttccc ttcgccgcag gcctcttcac ctggttgttt
    11101 ctgttctgga gaaaccgcca cgatgatcct gttgccaggg cacacaagcg ggcaggcagc
    11161 aaagcgttga agcaactgcg tgcagcgaag gaattatcgg aaggcgatga caccaaagcg
    11221 ttctgcaatg aagccatccg tgtcatctgg caatacctgg gaagcaaatg gcagctgcct
    11281 gtgagccggc agaacaagga acagatcacg cagatctttg ctgagaaaga tgcacctgat
    11341 gatctgcgtg atgagaccat tgaactgctg cgcaaactgg aaagaagcat ttatgctccg
    11401 gctatatcag gcagcagcag ccgcgaattg tttgaggaac tcgaatcctg gatcactcat
    11461 atggaaaaat ggcaggcatg agaaagtgga tactctccat agtagtgctg accaccctgc
    11521 aggcaactgc acaggacagt ttgtggcagg ccgccaacat tgcttatgag cagggcttct
    11581 ataagaatgc agacagcctt tataccatat tgttgaatga tcacggtcca tcagccgagg
    11641 tctattacaa tctgggcaat acctgctaca aacaggaaga ctggccgcgg gcgatattga
    11701 attatgaacg ttgtctgcag atagaccgca ggcataaaga tgccatcttt aacctggaat
    11761 tatccaacac ccatttgcgt gatcgcatcg atccgataga accatccttg ttgcatctct
    11821 tctggcgtga cctctgccac ctgttgccct ccggcacctg gggatggatc accctgtttt
    11881 tcgcctggtc ttcactggga tttttactgc tgttattatt cagtaaaaac ctgtcgctcc
    11941 gcaagaccgg attgtatggc atgttctctg cagcagccct caccatcctg ctgctggcca
    12001 tcacctatgc agcgcactat caggaagaaa gggaacagta tgccatcatc atgcagccga
    12061 gtcttatcct gaaaagtgaa cccagcgaaa gcagcaccaa cctctttgtg ttgcacagcg
    12121 gactcaaact tcgcatcctc gacagcgacg acagctggtc gctggtatcc atgcccgacg
    12181 gcaataccgg ctgggtgcag acggaagcgc tggagcagat ctagttcagg ttggcagttg
    12241 ccagtaggcg gttggcggtt acttttatcc gtgtattgtc tcaagttata gggagctgtc
    12301 gggagggtat ggtcagcaca tccgaactac tagtactcac tcccgatagc gatcactcac
    12361 aactgataga ggcgttcacc ctccgcattc cgcattgtcc tgactgccaa ctgcccaggg
    12421 tcaacttaaa aagtcatccc actgccacaa tactgtcacc acatactgtc ggccccggtt
    12481 tgacagttac taggtaaaag cgcttatgga aattggttac gcaccgcatc tattcattaa
    12541 ccatttaact aagcgccatg acaacaaaaa ccgcattcaa tcccttcgag cacagccgcc
    12601 tgttctttat ggcaggagtg gccatcagcc tcggcatcat ccagctggca ttcggctgga
    12661 acggtatgca ccggatccct caattgcctg atctgccaaa gatctctgac ggcacctggg
    12721 tggaggtcga atactttact gaaaaagtgg aagagcaact gcctgaaaag caggtgcaaa
    12781 aagaagaagt gatcattcct gaaccggatg cacccatcaa ggtggtggac gatgtaaaga
    12841 agatcaccac tcccattaaa ctcaataatc ccgaaaagga tcctgtgggt ttgaaaggcc
    12901 cttccagaac cgcgaccact acaacaggtg gcggaacaga gaagaaagag atcggtacca
    12961 tcgctgaagt gatgccggct ttccccggtg gatatgaagc tttgtacaag tggcttgcta
    13021 aaaacaccag ttatccgaag tgggcaaaag acgccggcat agaaggaact gtatatgtag
    13081 aattcattct gggtaccgat ggcaaagtgc aaagtgcaaa agtgctgcgt ggtatcggtg
    13141 gcggatgtga tgaagaagta ctgaatgccg tgctccgtat gcccgcctgg aatcccgcca
    13201 aacaagggaa tgtggaagtg ccactgcgca tgaccatccc cttcaccttc aagatttcat
    13261 agttgaattg ggttttctta tggttgtcat cagaccccgg ccttcgtgcc ggggtttttt
    13321 gtatcagaat acaaacaaca ctatgctgca caggatacgc aggaaacaat aaatgaagat
    13381 gggttcttta aaagaaaccc acctctgaca ccccacctta cacccaactc tgtcactatt
    13441 ctgccacaaa gaatttgctg aaatactttc tgactgaagc tgaagttcta acctcattag
    13501 cttatggcag aggtctacct ggttttcttc attcttcacc ttacaaccaa gcgctatgac
    13561 atctccaaca gcattcgatc ccttcagcca cacccgcttg ttcttcatga ccggtctggc
    13621 agtagcactc ctctttatcc tggcggcatt caggtgggat gtttccctgc cacaaagacc
    13681 aacacctgat caagaaaccg ctccggactt cggtgatccg atcaccctga tcgctcctgt
    13741 ggttgttcca ttaccggaac atccgttggt aatggaagca ccgaaaactc cacccatgat
    13801 agacctgtct gctgacatcc tggaagtacc ggatgagata cctctaaccg ctcggttgaa
    13861 ctttactgaa ccggatgaca cgccggttcc tctaaaagga ttcaccggca atactgtcct
    13921 gcctgaaatg ccatctgaaa cagacgatgc aaggttagca gcggaagtca tgcctgagtt
    13981 tcccggaggt aaagaagcat tgtataaatg gttgtccacc aatatcaaga tgccttctgt
    14041 tgccattgat ctcaacatga gcggagtggt ctatgtagac ttcgtgattg atacgaatgg
    14101 gaaggtgacc cgggtgaaga tcaggaaagg aactggtaca gttctggacg aggcagcact
    14161 ggaagccata caaaggatgc cggactggac accgggcatg cagggcggac gtccggttcc
    14221 ggtctttcag accttgccgg tgcggtttga actacgataa tgaaaaatag gtatgtgagt
    14281 ggtgaaccct gcctttggca aggagtgaat ggttagtttc tttgaaagaa gtaatactaa
    14341 ccattcacct ctcactgctc actttgctac agcgctccat caaaaatgaa aaatattcat
    14401 cgcgatcatg aagctcatga agcccaccag gtaacccagg tagatctctt tcttgctgtg
    14461 cgcactcagg atgagccggg aagtgcccac cagaccggca atgaggatga tcgtgagaaa
    14521 tggccagagc gcattgtact ggctgaaggg aatgagcagc aggaagaatg ccaccatacc
    14581 tccggcagca gtggtatgca tactcacttt cagaaacagg atgttgatca gaaatgccag
    14641 cacgatggag atgcagctgg ccagcagtaa aaaggtgacc atgccattaa agctttcact
    14701 gtagaacaca taaaatgccc agatgtaaaa agccatggtg gcgatgtaag gcactacacg
    14761 atcctgcctg tctcggagtg aaatggagcc gatgaatttc agcgccgaca tcagcccgac
    14821 aaaaaccgcc ggaaagaaaa aggtgagcag tgctacccgc aacaacaaca tgaaattctc
    14881 acccggatcg ggataagaaa agggattgct gtacaacagg tagaaggtgc ccagcaaggg
    14941 catca
//
LOCUS       DAOMAX010000043        14802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_477_length_14802_cov_4.205872, whole genome shotgun sequence.
ACCESSION   DAOMAX010000043 DAOMAX010000000
VERSION     DAOMAX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_14802_cov_4.205872"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK742_03880"
     CDS             complement(<1..297)
                     /locus_tag="PK742_03880"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75759.1"
                     /translation="MTRFIAARLLQGFLVMLGVSVLVFFLFQLVRFNPAYALAGEAAS
                     TETVANISRELGLDRPVGAQLLTHLNNLSPLSRHALDPAARNYLEPGSVKAITIM"
     gene            complement(307..1962)
                     /locus_tag="PK742_03885"
     CDS             complement(307..1962)
                     /locus_tag="PK742_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HQU75760.1"
                     /translation="MRSFILGLSRFLVGALFIFSGLIKANDPVGFAIKLEEYYDIFAS
                     GGGILSFFHSSIILNTVVYQAAFICILEVALGVLLLLGMWPRLVSWLLLLMIIFFTWL
                     TGFSAFTGQVTDCGCFGDAIPLTPLQSFYKDLVLMVLIIIIFAGRNRINRLLPAVLSF
                     AIFFATTAFSIWVVNSVLKYDVFIDFRPYKVGNNIAEQMAIPDDAPAPVVEMQYIYRN
                     KQSGKEGVAKIRSDENNMDALKPFGDSNTWEFVERKDKVIDAGFIPKITDFAVLHEDG
                     EDITDQVLHFDDYLIMVVSAGLDHTERSAWDGINELQQAAEAEGISTFGLVSSNRKDI
                     EKFRHNHQTAFPFYQGDHKVCLAIARTNPNILLLKNGTVVAKWPWRETPSFNEMKSMY
                     FPDRPATEITFLQNETSGLFSTGEDVVSKLENSTEPYNEFFLMDAAGNDLAYDMLAES
                     GPHYMVIIADMTQLTREVFASMQPVLQELENRQAHYFVVSGSSLGSLQQMQDATGLHF
                     SFFNSDAEVLGKIVETNTGMVVVQDGRVVAVYDEANFPVAEEL"
     gene            complement(1968..2522)
                     /locus_tag="PK742_03890"
     CDS             complement(1968..2522)
                     /locus_tag="PK742_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HQU75761.1"
                     /translation="MNPEANHTSLQYDEVVKACRDIFSKKIRDYGTSWRVLRPSSLTD
                     QIFIKAQRIRTVEQTLERRVSDTIEDEFIGIINYSIIALIQLELPEDAPVHLAEQQAL
                     QMFDSHFSGIKSLMENKNHDYGEAWRSMRTSSFTDLILMKIMRIKQIEENDGQTLISE
                     GVASHYMDMVNYSVFALIQLKEKA"
     gene            2537..3430
                     /gene="folP"
                     /locus_tag="PK742_03895"
     CDS             2537..3430
                     /gene="folP"
                     /locus_tag="PK742_03895"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQU75762.1"
                     /translation="MQPKTTYFPTLPTLETKAGKVDLSTPKVMGIINATPDSFFSESR
                     VARVEEAVQKAADMVSEGARMLDIGGMSSRPGADIIDPKVEATRVIPIVQAVAQAFPH
                     IPVSIDTIHADVAEAAVEAGASIINDISAATWDRRMTAVAALTGAPVILMHMQGTPAL
                     MQNDPHYENVALEVREYLLRRCDALKTAGVEQLILDPGFGFGKTLEHNYQLAAGCRQL
                     ADTGMPLLVGVSRKSMVNKVLNISSNNALTGTIALHMYLLQQGASILRVHDVREAVET
                     VAIFRATRAGQLHENVPLLSA"
     gene            3427..4242
                     /gene="cdaA"
                     /locus_tag="PK742_03900"
     CDS             3427..4242
                     /gene="cdaA"
                     /locus_tag="PK742_03900"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQU75763.1"
                     /translation="MNLTLLFRIDFLEIGLTDVLDIVLMSLLIYQLYRIVRGSLAYNI
                     FFGILAVYLLSLLADAFDMELMKGVLGQFINIGVLGLLIVFQPEVRRFLLQLGRNSDI
                     RKPAFWRRFNLRSYQQHQSEYREVEELIKAVLQLSTEHTGALLVIPRTSRLQFFANSG
                     VQVQARIDADLLQSIFIKNGPLHDGAVIISDGRIQAARCVLPVSENTSGLPGHFGLRH
                     RSALGITEVSDATAIIVSEENGHISYAKDGQLFQNIAEETLRKVLTDALFGRG"
     gene            complement(4225..4800)
                     /locus_tag="PK742_03905"
     CDS             complement(4225..4800)
                     /locus_tag="PK742_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75764.1"
                     /translation="MKFRLPIFLILFISVAIVSCNNADKQKERIADLQMEMDSVFAST
                     RELTPSQKEKAHELIDAYLQYADQHQQDPNAAAYMFEAARLTTALPEYERAIEIFRLI
                     VNKFPESEYAPRSLLSIGGIYDVSIGDYEQAKKAYEELNEKYPLQAEEYDVPAILRTL
                     GMPADQILRQLRSANGTADSMDIRNGSASAE"
     gene            4839..6122
                     /locus_tag="PK742_03910"
     CDS             4839..6122
                     /locus_tag="PK742_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQU75765.1"
                     /translation="MYKVSFIAIILFALTACSPRYHKTFIGEDDIQSTFIKGERYTCD
                     DWENYIPNGYTPMRYVRVNFHFMLDENGEYNFPEQQARTYIRDLVRACNDKLADNKQM
                     NLPEGNDTPALPTRYRIVLTGDPSTGSDGIYFHRDDTACFFDKQKNSGRYGLFDKSMF
                     QYGVGQDSIINIFFLEHHPDSVAAHDDFNVTASGISFATNIKIFGAYYNATTTFYGDE
                     GQPFNKGAWFYAGLVNHEIGHTLGLSHTWRTDDGCDDTPKNPGCWGPNDPPCNGINSN
                     NMMDYNTCQCAISPCQLGKIHANFNMTGSRQRAVLQEKWCDYDPYNRAHIARNTTVTF
                     QGYHDLNSDVVIEEGATLIVRCRLALPADARIIVRPGGTLIIDGGTITNICGEEWEGI
                     EVWESIKYGIKGEVKVARGGIVENARNFTRKTLEE"
     gene            complement(6173..7228)
                     /locus_tag="PK742_03915"
     CDS             complement(6173..7228)
                     /locus_tag="PK742_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75766.1"
                     /translation="MKNAIVTTLILTAGSLLYAQEPAAVPAAMEEPAKVIVTITENGE
                     TYTIELPSLESLADMEDLKGLETLQYLEGLEDEDLEFDTFLAEVQADTLDSTSVTIGK
                     WKVVVKEKGDGTDDVDVKFDRVEDDDVKDIDTYRDVDVFETDWFLFDLGYNTYVDKDM
                     QFVKLMADSIALDQKSWGSWDVNLHIFRQRLNIAQGFVNLNWGLSFEWHNYMFRGNYY
                     PTPQQSVFTLQESASTTNLEKNRFSSTHFTLPVMLGFETKPWDTDHSFRMGFGYSPGL
                     MVKGKTKIKDDRGTNKVKDDFNLESPFRHEVNVMMGYGGFNLYASYDLNSMFAEGEGP
                     ELHPVSVGLIIRRGFDN"
     gene            complement(7231..8052)
                     /locus_tag="PK742_03920"
     CDS             complement(7231..8052)
                     /locus_tag="PK742_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75767.1"
                     /translation="MKLNLDNYESWLVDRMEGLLDPAQEAELDAFLELHPELQEDAAL
                     MEMTRLVPDQEVIFTDKASLKKEEGGRILALFTMGRLSMAAAAVLLAFVGIRFLTNPA
                     ESTLATGYQSAAQQAAVPVMAASAESLEARDSVPGIRHQGAPVQFAVQEKKMVEEKKQ
                     PRETQFANYLNPVQLQQLPAVEKRPVLHLNDFEEQYALINNYDYENMMKGTTSGSGPE
                     TIVDKMNDAMAIANEMSSVLGFGKKDEEEHSGSHMRTTSIRILGIEYYNRKRINQ"
     gene            complement(8049..8537)
                     /locus_tag="PK742_03925"
     CDS             complement(8049..8537)
                     /locus_tag="PK742_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQU75768.1"
                     /translation="MTTTEYNECVELWADGVFRFIVKNLRHEEDARDVVQNAFEVLWK
                     NHSDIDAAKAKSYLFSTAYHNMIDLIRKQKRVDYVEEVEEGARGGTSAYQVDLKEALD
                     KALARLPEIQRNAVLLRDYEGYSYEEIGEILELTESQVKVYIFRARTTLKKYLVSIHH
                     LI"
     gene            8658..9503
                     /locus_tag="PK742_03930"
     CDS             8658..9503
                     /locus_tag="PK742_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU75769.1"
                     /translation="MTILFPTDFSEASANAFGMVAVLAEKTGLPVTLIHIENPPIISP
                     AYDDAGMSMVTDTLLADMDTMVHNRIAAFRDELNTRFHLDQRNVGLQEEVRIGFHGEE
                     IARAAEELDAAYIVTSVRDVPLVNRVLFGGTVNAILRHTNRPVITLPEHQPLHQFQRI
                     GYATDFSLDDGALITRLLDFARLYDARVYCFHVHDSNLDVEYDILRELEEMYSTEITE
                     GRITFYLQENLRILDGLEYFIQEHNLDMLAMMRQKHYWPEFLNGSHTRSMALHARIPM
                     LIFHE"
     gene            9569..10387
                     /locus_tag="PK742_03935"
     CDS             9569..10387
                     /locus_tag="PK742_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HQU75770.1"
                     /translation="MSAKRPFRIAILDMYNNHPNEGLRNIKEIVSEEYLKAEWNVYDV
                     RGKGEVPGLDYDVYISTGGPGSPYDGEDKQWEQDFFQLMDGIWNNNANSQEEKKYVFA
                     ICHSFQLLARHFNLGKVCKRKSTAFGVFNIHKTENARKDPYFRTLPDPFYAVDSREWQ
                     LIQPNAEEVISHDAVVLAIEKRRDHVPYERAIMAMRLTPYIYMTQFHPEADASGMLHY
                     FKMPEKKQHVLEHHGDWKLEEMIMHLSDPDKIPLTHEALLPAFLRDARETLSLT"
     gene            10389..11570
                     /locus_tag="PK742_03940"
     CDS             10389..11570
                     /locus_tag="PK742_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75771.1"
                     /translation="MIQDIRRQYNGKFTQEAYDQFFRSLWEHFHQPIEFKVCETPVFV
                     PEWLRNLLIQAGEEVIDTLVQPGFKEATEASIPDHLRVPGEDDHTTFLAIDFGICKVG
                     DEILPQLIEMQGFASLYGHQHAMARGYRRFFDIPGHMSHLFSLEDLEYEDLLRRTLLG
                     DHKAENVILLDIEPNKQKTWVDFWDTKFMTGIEPVCITELHRSGNDLWYERDGQKINV
                     HRIYDRLIFDELEARKDLRYDWNKTEPVNVEWVSHPNWFFRISKYTMPMLRSRFVPET
                     HFVHELPVIPDDLENYVLKPLFSFAGAGVKFDVQRDDISSLEHPEHFILQKKVQYEPV
                     IQTPDVPAKFEVRMMYIWEKDSPRPRLVINLVRLSKGVMIGVNFNKNKDWVGGSVGFF
                     E"
     gene            complement(11550..12134)
                     /locus_tag="PK742_03945"
     CDS             complement(11550..12134)
                     /locus_tag="PK742_03945"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQU75772.1"
                     /translation="MNKVQIYLASGSPRRQQILRDAGFDFQLWKMDVDEQFPPEMPAE
                     DVPSYLAAKKMYAAARQLHDKDGVIITADTVVILNGRIIGKPEDEADACNILSRLAGQ
                     THTVITAVCLRKGASEQIITSETKVFMLPMSMEEIRHYVVTARPLDKAGAYAIQEWVG
                     LARISRIEGDYYNVVGFPMSAVYPHLKALLEESD"
     gene            complement(12131..13066)
                     /locus_tag="PK742_03950"
     CDS             complement(12131..13066)
                     /locus_tag="PK742_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HQU75773.1"
                     /translation="MNRAWRIVRPVNLGIVALTFVFIRLFLFQPVTKNGSPSLYLQNW
                     EYSLLAISVILITAAGYIINDIRDVEADRINKPDRQFVGSSVSESLAWRYYFILTIAG
                     LISGLFISLRVGRLPLFGVHVMAVLLLYLYAGFLKRVALAGNLTVSVLIAISVLTPAV
                     FEPAFYEVENLSEYQTAAYVWKWVAGLAVFSFLLTLPRELVKDLEDLEGDRLAGFRTF
                     PALAGEQATRVLVICWLVVVSALTVYTSRYLWLFYDDRSFLVIGGVMMFGLCWMMLVL
                     SKPVTLQLNHLVSLVSKLMLLPGLLSLWVQYLIIK"
     gene            complement(13066..13305)
                     /gene="iscX"
                     /locus_tag="PK742_03955"
     CDS             complement(13066..13305)
                     /gene="iscX"
                     /locus_tag="PK742_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HQU75774.1"
                     /translation="MVFEEPPIHWNDYEDIALKLYERFGDDFNEGKIYRIRFTELIEW
                     VLEIDGFHGTREECNEGHLEMIQSSWVYEWRDNQS"
     gene            complement(13317..13652)
                     /locus_tag="PK742_03960"
     CDS             complement(13317..13652)
                     /locus_tag="PK742_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQU75775.1"
                     /translation="MGNYSIRFVFEDGSEPITVEAKTGRTILEVALLNDIELNHNCGG
                     VCACSTCHVYIEKGMESLEEITDKEEDFVDRARDPRLHSRLSCQCDLLEGDGEIEVIV
                     PDQSTIIAS"
     gene            complement(13667..14452)
                     /locus_tag="PK742_03965"
     CDS             complement(13667..14452)
                     /locus_tag="PK742_03965"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HQU75776.1"
                     /translation="MDHTKDIRDLTIVLAGTGRLAWHWPDMLALAGLRVAYVLGRSTD
                     GVQELAAKAGSFGIMDPAHLPQAEDLFVILAVSDAAIQEVAGSLKGERRMMIHCSGSA
                     VSEGITDGVCYPLQTFSRERQPVYNNIPFFLTAYRPDTEAILKQLVLMINGKFRFIDD
                     DTRSRLHLAAVVVNNFTNHLVQLASEYLHGAGMSYQDLLPLLEETVYKLEEMDPGSAQ
                     TGPAVRRDLEVISKHLQMLQNHPALQDVYELMSRNLQEKYRHH"
     gene            complement(14454..>14802)
                     /locus_tag="PK742_03970"
     CDS             complement(14454..>14802)
                     /locus_tag="PK742_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75777.1"
                     /translation="YQTGDTIAAGAQFRELNGNESAVVEPQFLIYGSQTGTLPAELVY
                     AGGTIVFQMIQPETDTFIFQTREQNVPQDWIIMKAIVFPMINLVWLGMIVLAIGFAIS
                     MRKRLEDLRRRKG"
BASE COUNT         3776 a         3703 c         3544 g         3779 t
ORIGIN      
        1 cataatagta atggctttaa cagaccccgg ttccaggtag tttcttgctg caggatcaag
       61 cgcatgtctg ctaagcggcg acaggttatt gagatgagtc agcaactggg cacccacggg
      121 ccggtcaagt cccagttcac gactgatgtt ggcaaccgtt tcagtggaag cggcctcacc
      181 agcaagtgcg taggcagggt tgaagcgaac aagctgaaac aggaaaaata ccagcacact
      241 gacccccagc atgaccagga aaccctgcag gagccgtgct gcgataaatc ttgtcaatag
      301 tatcaattac agttcttccg caacaggaaa attggcttca tcatacacgg ctactaccct
      361 tccatcctgc accaccacca taccggtatt ggtctccacg atcttgccca gcacctctgc
      421 atcgctgttg aagaaggaga aatgcagtcc cgtggcatcc tgcatctgct gcagacttcc
      481 cagactggaa ccactaacca caaaatagtg tgcctgcctg ttctccagct cctgcaaaac
      541 aggctgcata ctggcgaaga cctctcttgt aagctgggtc atatcagcaa tgatgaccat
      601 ataatgcgga ccgctttctg ccagcatatc ataggccagg tcattccctg cggcatccat
      661 caggaagaat tcattgtacg gctctgtgct gttctccagt ttgctcacca catcttctcc
      721 ggtgctgaag agaccggaag tttcattctg aaggaaggtg atctctgttg ccggcctgtc
      781 gggaaagtac atggacttca tctcattaaa tgagggtgtt tctctccacg gccacttggc
      841 aacaaccgtt ccattcttca gcaaaaggat gttgggattg gtgcgggcga tggccagaca
      901 aaccttatga tcgccctgat agaatgggaa agctgtctgg tgattatgcc tgaatttttc
      961 aatgtccttg cgattgctgg acacaaggcc gaaggtagaa ataccttctg cttcagcggc
     1021 ttgttgcagt tcattgatac catcccaggc agatctctct gtatggtcaa ggcctgcaga
     1081 gaccaccatg atcaggtaat catcgaaatg aagcacctga tctgtaatat cttccccatc
     1141 ttcatggagt actgcaaagt ctgtgatctt tgggataaag cctgcatcaa tgaccttgtc
     1201 tttgcgttcc acgaactccc aggtattgct atcgccgaaa ggtttcagcg catccatgtt
     1261 gttctcatcg ctgcggatct ttgctacccc ctctttgcca ctttgtttat tccggtagat
     1321 gtactgcatc tccaccacag gagccggagc atcatccgga atggccatct gctctgcaat
     1381 gttgttgccc actttgtacg gacggaagtc tataaataca tcgtatttca gcactgaatt
     1441 cactacccag atactgaagg ctgttgtggc aaagaagatg gcaaatgaca ggacagcagg
     1501 gagcagccgg ttaatgcggt ttctacctgc gaaaatgatg atgatcagca ccatcagaac
     1561 cagatcctta tagaaggatt gcaggggcgt gagcggtatg gcatctccaa agcagccaca
     1621 gtcggtcacc tggccggtga aggcagagaa gcccgtcagc caggtaaaaa atatgatcat
     1681 gagcagcagc aaccaggaaa ccaaccgggg ccacataccc agcaataaca gcaccccaag
     1741 tgccacttca agaatacata taaaggctgc ctgatagaca actgtattca ggataatgga
     1801 agagtgaaag aaagaaagga tgccgccacc tgaagcgaag atgtcatagt attcttccag
     1861 tttgatggca aaacccaccg gatcattggc tttgataagc ccggaaaaaa tgaacagggc
     1921 tccaaccaga aagcgggaaa gaccgagaat gaatgacctc atagtgctta tgctttttct
     1981 ttgagctgaa tgagtgcaaa cactgaataa ttgaccatat ccatatagtg ggaggccact
     2041 ccttcagaga tcagagtctg cccgtcgttc tcttctatct gtttgatgcg catgatcttc
     2101 atgaggatca gatctgtaaa ggaagaggta cgcatacttc gccaggcctc tccatagtca
     2161 tggttcttgt tttccatgag tgatttgata ccggaaaagt ggctgtcaaa catctgaagt
     2221 gcctgttgtt ctgccagatg aacaggtgcg tcttcaggca gttccagctg gatgagggca
     2281 atgatggaat agttgatgat cccgatgaat tcatcttcga tcgtatcact aaccctgcgc
     2341 tccagcgttt gctcaaccgt gcggatgcgc tgtgctttga tgaagatctg gtcggtcagg
     2401 ctggacggac gcaacacccg ccaggaggtt ccgtaatccc gtattttctt ggagaatatg
     2461 tctctgcagg ccttcaccac ttcgtcatac tgcaggctgg tatggtttgc ttccggattc
     2521 attagatttg gctcagatgc agcccaaaac tacatatttc cctacgcttc cgaccctgga
     2581 gacgaaggct ggaaaggtgg acttatccac accaaaagtg atgggcatca tcaacgccac
     2641 gcctgactcc tttttctctg agagccgggt ggcccgggta gaagaagccg tacagaaagc
     2701 tgcagatatg gtaagcgaag gcgcaaggat gctggatatt ggaggcatgt cttcacggcc
     2761 gggggctgac atcattgatc cgaaagtaga ggcaacccgt gtcatcccga tagttcaggc
     2821 agttgcccag gcatttcctc atataccagt atcaatagat accatccatg cagatgtggc
     2881 agaagctgct gtagaagcag gtgcatctat catcaatgat atcagtgcgg ccacctggga
     2941 caggcgcatg accgctgttg ctgcactgac gggggctccg gtcatcctca tgcacatgca
     3001 gggtacgcct gccctgatgc aaaacgatcc tcactatgaa aatgtggcct tagaagtacg
     3061 tgaatatttg ctgcgcaggt gtgatgcgct taaaactgca ggtgtggaac agcttattct
     3121 ggatccggga ttcgggtttg gaaagaccct ggaacacaac tatcaactgg cagcaggttg
     3181 caggcagctg gctgatacag gaatgccgtt gctggtaggt gtttcaagaa aatctatggt
     3241 caataaagta ttgaatatca gttcaaataa tgcacttacc ggaaccattg ctttacatat
     3301 gtatctgttg cagcagggcg cttctattct gagggtacac gatgtgaggg aagcggtaga
     3361 gaccgttgct attttccgtg ccacccgtgc cgggcaactg cacgaaaatg tacctttgct
     3421 gtcggcatga acctcaccct cctgttccgg atagatttcc tggagatcgg cctgaccgat
     3481 gtgctggaca tcgtgctgat gagcctgctc atataccagt tataccgaat tgtaagaggc
     3541 agcctggcct ataatatctt ctttggcatc ctggccgttt acctgctgag cctgctggcc
     3601 gatgcctttg atatggagtt gatgaagggg gtacttggcc agttcatcaa tatcggcgtg
     3661 ttaggattgc tcattgtttt tcagccggag gtcagacgct ttctattgca gcttggcagg
     3721 aacagtgata tccgcaaacc tgccttctgg cgacggttca atctcaggtc ctaccagcag
     3781 catcagagtg aatacagaga ggtagaagag ctgatcaagg cagtacttca actatcgacc
     3841 gaacatacag gagccctcct tgtgattccc cgcaccagca ggctgcagtt ctttgccaat
     3901 tcaggggtac aggtgcaggc ccgtattgat gcagatctgc tccagagcat tttcattaag
     3961 aatggtccgc tgcatgatgg ggcagtgatt attagtgacg gccgcataca ggctgcccgt
     4021 tgtgtgctac cggtaagtga aaataccagc gggctaccgg gacatttcgg cttgcggcac
     4081 cgcagcgccc tgggcatcac ggaagtaagt gatgccactg ccatcattgt ttcagaagag
     4141 aacggacata tttcttatgc caaagacgga caactttttc aaaacattgc agaagaaacc
     4201 ttgcggaaag tgctgacaga tgcgctattc ggcagaggct gatccattcc tgatgtccat
     4261 actatcggcc gtaccgttgg cacttctcag ctgacgcagg atctgatctg ccggcatacc
     4321 cagcgtgcgc agtatggcag gcacgtcata ttcctccgcc tgtaacggat acttttcatt
     4381 caattcttcg taagcttttt ttgcctgctc atagtcgcct atggaaacat cgtaaatgcc
     4441 accaatggac agcaggcttc tgggggcata ttcagattcc ggaaacttat tgacgatcag
     4501 tctgaatatt tcaatggctc tttcatattc gggtaaagca gtggtcagcc tggcagcctc
     4561 aaacatatag gctgcagcat tgggatcctg ctgatgctga tctgcatact gcaaataagc
     4621 atctatcagt tcgtgtgcct tttctttctg agaaggcgtc agctctctgg tactggcaaa
     4681 aacgctgtcc atttccatct gcaggtctgc gatacgttct ttctgcttat ctgcgttgtt
     4741 acaggatacg atggcaacag aaatgaacag aatgaggaat atcggtaaac gaaacttcat
     4801 atttttatcc ggttttctgc aaaggtaata gccagaatat gtataaagtc agtttcatcg
     4861 ccataatctt gtttgccctg acggcctgct ctccccgtta ccataagaca ttcattggcg
     4921 aagatgatat acagtccacc ttcatcaaag gcgagcgata cacctgcgac gactgggaaa
     4981 actatatacc gaacggctac acccctatgc gttatgtacg ggtcaacttt catttcatgc
     5041 tggatgagaa tggcgaatac aacttcccgg aacaacaggc ccgcacctac atacgtgatc
     5101 tggttcgtgc ctgcaatgat aagctggcag acaataaaca aatgaatctt cccgaaggga
     5161 atgacacccc tgcccttcct accaggtacc gcattgtact tacgggcgac ccttccaccg
     5221 gaagtgacgg catatatttt caccgcgatg atactgcctg cttttttgac aagcaaaaga
     5281 acagcggtcg atacgggctg tttgacaaga gcatgttcca gtatggtgtg ggacaagaca
     5341 gcatcatcaa catttttttt ctggagcatc acccggacag tgtagccgcc cacgatgatt
     5401 tcaatgtcac tgccagtggc atttcctttg caaccaatat caagatcttt ggtgcatact
     5461 acaatgccac tactaccttt tacggtgatg agggtcaacc attcaataaa ggagcctggt
     5521 tttatgccgg gctggtgaat catgagatcg gtcacacact tgggctcagc cacacatggc
     5581 gtaccgacga tggatgtgat gataccccga aaaatccggg atgctggggt ccgaatgatc
     5641 cgccgtgcaa tggcatcaac agcaacaata tgatggacta caacacctgc cagtgcgcca
     5701 tttcaccttg tcagctggga aagatccatg ccaatttcaa catgaccgga agcagacaac
     5761 gggctgtgct acaggaaaaa tggtgtgatt atgatccata taacagagcc catattgcca
     5821 ggaacaccac tgtgaccttt cagggatacc atgacctcaa cagtgatgtg gtcattgaag
     5881 aaggagctac actgattgta aggtgcaggc tggcattgcc ggcagatgcc aggatcattg
     5941 tgagacccgg cggcaccctg atcattgacg gaggtaccat caccaatatc tgcggtgaag
     6001 aatgggaagg cattgaggtg tgggaaagca tcaaatacgg aatcaaagga gaagtgaaag
     6061 tggcccgcgg gggaatcgtg gaaaacgcca ggaattttac cagaaagacg ctggaagaat
     6121 aaaaaagagg acgagtttcc ccgtcctcct atccaacacg ataaaagtac gcttagttat
     6181 cgaacccgcg acggatgatc agaccaactg acaccgggtg gagttcaggt ccttcgcctt
     6241 ctgcaaacat gctgttcaga tcatagctgg cgtacaggtt gaaaccgcca tatcccatca
     6301 tcacattcac ttcatggcgg aaaggacttt ccaggttgaa atcatctttc actttgttgg
     6361 taccacggtc atctttgatc ttggtcttgc ctttgaccat cagtcccggg ctgtaaccaa
     6421 atcccatacg gaaagagtgg tcagtatccc agggtttggt ctcgaaaccc agcatgaccg
     6481 gcagggtaaa atgggtggaa ctgaatctgt ttttctccag attggtggta gaagcagact
     6541 cctgtaaagt gaagacagat tgctgcggag taggataata attgccgcgg aacatatagt
     6601 tgtgccattc aaaactgagg ccccagttca ggttcacgaa accctgggcg atattgagcc
     6661 gctggcggaa gatgtgcagg ttcacatccc agctgcccca gcttttctga tccagagcaa
     6721 tgctgtctgc catcagtttg acaaactgca tgtctttatc cacgtaggtg ttgtaaccca
     6781 gatcaaagag gaaccagtct gtctcgaata catctacatc gcggtaggta tcaatgtcct
     6841 tgacatcgtc atcttccaca cggtcgaatt tgacatccac atcatctgta ccatctcctt
     6901 tttccttgac cacaactttc catttaccga tggtcacaga agtactgtcc agtgtatcag
     6961 cctgtacttc tgccaggaaa gtgtcgaact ccagatcttc atcttccagc ccttcgaggt
     7021 actgcagggt ttccagtcct ttcaggtctt ccatatctgc aaggctttcc aggctgggaa
     7081 gttctatggt ataggtctct ccgttctcgg tgatggtgac gatcaccttt gcgggttctt
     7141 ccattgctgc aggaacagcg gcaggttcct gtgcatacag caggctgcct gctgtcagga
     7201 tcagggtagt tacaattgcg tttttcatgc ttactggttt atgcgtttac ggttataata
     7261 ttcaattcca aggatgcgga tggaagtagt tctcatatgg gaaccactgt gttcttcctc
     7321 atcttttttt ccaaagccca gcacagaact catctcattg gcgatggcca tggcatcatt
     7381 catcttgtca acgattgttt caggaccgct tccggaagtg gtacctttca tcatattctc
     7441 gtagtcgtaa ttattgatca gagcgtactg ttcttcaaag tcattgagat gaagcaccgg
     7501 tcttttctca acagcgggaa gctgctgcag ctgcaccggg ttcagatagt tggcaaactg
     7561 tgtttcacgg ggctgttttt tctcttccac catctttttc tcctgaacag caaactgaac
     7621 aggtgcaccc tggtggcgga tgccgggcac tgaatcacgt gcttccagac tttctgcgga
     7681 tgctgccatg acgggcactg ctgcctgttg tgcggcactc tggtatccgg tggccagggt
     7741 gctttcggcc ggattggtca gaaatctgat tcccacgaat gccagcaaca cagctgctgc
     7801 tgccatagaa agccgcccca tggtgaacag ggcaagtatt cttccgccct cttccttctt
     7861 cagagatgcc ttatctgtaa aaatgacttc ctgatcaggc acgagtcgtg tcatttccat
     7921 cagggcagca tcttcctgca gctccggatg cagttccagg aacgcatcca gttcggcttc
     7981 ctgagcagga tccagcagac cttccatgcg gtcaaccagc cagctttcgt aattgtcaag
     8041 attcagtttc atatcagatg atgtatactg accaaatatt ttttcaaagt tgtcctcgcc
     8101 cggaagatgt acaccttcac ctgcgattcc gttaattcca ggatctctcc gatctcttcg
     8161 tagctgtagc cttcataatc acgcaggagt accgcattcc gctgtatctc cggcagtctg
     8221 gccagcgctt tgtccagcgc ctctttcagg tccacctggt aggccgaggt tccaccccgt
     8281 gctccttcct ccacttcttc cacataatcc acccgctttt gcttccggat cagatcgatc
     8341 atgttgtgat aggcggtact gaacaagtaa gacttagcct ttgccgcatc tatatcacta
     8401 tgattcttcc aaagaacttc aaatgcattc tgcaccacat ctcttgcatc ttcctcatgc
     8461 ctgaggtttt tgacgatgaa ccggaatact ccgtccgccc acaattcaac gcattcgttg
     8521 tattcggttg ttgtcatctg tccgttagtc gttgaagggg gtgcaaaggt tacagggaaa
     8581 cgtaaatttt ttatttacct gactgaaatc accgtttttc gggcctggag aggttaaatt
     8641 taaggaaaat caatgctatg accatcctgt ttccaaccga cttcagtgaa gcttctgcca
     8701 atgcttttgg tatggtggcc gtactggcag aaaagaccgg gttaccggta acgttgatcc
     8761 atatagagaa cccgcctatc atcagtcctg cttatgacga tgccggcatg tcaatggtaa
     8821 cagacacctt gctggcagac atggatacga tggtccacaa caggatagct gcattccgtg
     8881 atgagctgaa caccaggttc cacctggatc agcgtaatgt tggtctgcag gaagaagtgc
     8941 gcatcggttt tcatggagaa gagatcgcca gagcagcaga agaactggat gcagcctata
     9001 tcgtaacaag tgttcgtgat gtacccttgg taaaccgcgt actgtttgga ggcactgtca
     9061 atgccatatt gcgccacacc aaccgtccgg tcattaccct tccggaacac cagcctttgc
     9121 atcagtttca gcgcataggc tatgcaacag atttcagcct ggacgatggt gccctgatca
     9181 cccggctgct ggactttgcc agactgtatg atgcccgtgt gtactgcttc cacgtgcatg
     9241 attccaatct ggatgtggaa tatgacatcc ttcgtgaact ggaagagatg tacagcacag
     9301 agatcacgga agggcggatc accttctacc tgcaggaaaa tctgcgcata ctagacggtc
     9361 tggagtattt cattcaggag cacaatctgg atatgctggc catgatgcga cagaagcatt
     9421 actggccgga gttcctcaac ggcagccata cccgttccat ggcattgcat gcccgcatac
     9481 cgatgctgat cttccacgaa taagggtgtg tacattcctg gtctataaag aataagaagg
     9541 aacttacctt tgtggctgat cgtgttgtat gagtgcgaag agacctttcc gcattgccat
     9601 cctggatatg tataataacc atccgaacga agggttgcgg aatatcaaag agatcgtgtc
     9661 tgaagagtac ctgaaggcag aatggaatgt atatgatgtc agggggaaag gtgaagtacc
     9721 tggcctggat tatgatgtct atatctctac cggaggcccg ggcagcccct atgacggcga
     9781 agacaaacaa tgggagcagg actttttcca gctcatggac ggcatctgga ataacaacgc
     9841 caacagtcag gaagagaaga aatatgtgtt cgccatttgt cactcctttc aattactggc
     9901 aagacacttc aacctgggga aggtctgtaa gcggaagtca actgcctttg gtgttttcaa
     9961 catccacaag acggaaaacg ccaggaaaga cccttacttc agaaccctgc cggatccttt
    10021 ttatgccgta gattccagag aatggcagct cattcaaccc aatgctgaag aagtgatcag
    10081 ccacgatgcg gtggtgctgg ccattgaaaa acgcagagac catgttccgt atgagcgggc
    10141 catcatggcc atgcgcctca ccccttatat ttatatgacc cagtttcatc cggaagctga
    10201 tgccagtggt atgctgcatt attttaagat gcctgaaaag aagcaacatg tgctggagca
    10261 tcacggcgac tggaaactgg aagagatgat catgcatctg agtgatccgg ataagatacc
    10321 gctcacccat gaagcattat taccggcatt cctgcgagat gccagagaaa ctttaagttt
    10381 gacctgagat gatccaagat attcgccggc aatacaacgg gaaattcaca caggaggcct
    10441 atgatcagtt cttccggagt ttgtgggaac atttccatca accgattgaa ttcaaggtat
    10501 gtgaaacgcc tgtctttgtt ccggaatggc tgcgcaacct gcttatccag gctggcgaag
    10561 aggtcataga caccctcgta cagccggggt ttaaggaagc tactgaagcc tccattcctg
    10621 accacctgcg ggtacccgga gaagatgacc acaccacttt tctggccatt gacttcggca
    10681 tctgcaaagt gggtgatgag atccttccgc agctgatcga gatgcagggc tttgcatcat
    10741 tgtacgggca tcagcatgct atggccagag gataccggcg tttcttcgac attccgggtc
    10801 atatgagcca cttgttttca ctggaagacc tggaatatga agacctgctt cgccgcaccc
    10861 tgctgggtga tcataaagca gagaacgtga tcctgctgga tattgaaccc aacaaacaga
    10921 agacctgggt tgatttctgg gataccaagt tcatgaccgg tattgagccg gtgtgcatta
    10981 ctgagctgca tcggtcagga aatgatctgt ggtacgaaag agacgggcag aagatcaacg
    11041 tgcaccgtat atatgaccgg ctcattttcg acgagctgga agcaagaaaa gatcttcggt
    11101 atgactggaa caagacagaa cctgtcaacg tggaatgggt gagtcatccc aactggttct
    11161 tccgtatcag taaatatacc atgcccatgc tgcgcagccg tttcgttcct gaaacgcatt
    11221 tcgtgcatga attaccagtc attccggatg acctggaaaa ttatgtcctc aaacccctgt
    11281 tctcttttgc aggcgccggt gtgaagtttg atgtgcagcg tgatgatatc agttcactgg
    11341 aacatcctga acatttcatt ctgcaaaaga aagtgcagta tgaacctgtt attcagactc
    11401 ctgacgttcc tgccaaattt gaagtgcgca tgatgtatat atgggaaaaa gacagccctc
    11461 gcccaagact ggtgatcaat ctggtcagac ttagtaaagg agtgatgatt ggcgtgaact
    11521 tcaacaagaa taaagactgg gtaggcggat cagtcggatt cttcgagtaa tgctttcaga
    11581 tgcggatata ccgcactcat tggaaagccc accacattat aataatcgcc ttctattctg
    11641 ctgatcctgg caagccctac ccattcctga atggcatagg caccggcctt atccagcggc
    11701 ctggcagtga ccacataatg tctgatctct tccattgaca tgggcagcat aaacactttg
    11761 gtttctgacg ttatgatctg ttcagaagct ccttttcgca aacaaaccgc tgtgatgacc
    11821 gtatgtgtct ggccagcaag tctgctgagt atattacagg catctgcttc atcttccggc
    11881 tttcctatga tgcgaccgtt gagtatgacc acggtgtctg cagtgatgat gaccccgtct
    11941 ttatcatgca gctgtctggc tgctgcatac attttttttg ctgccagata agatggcaca
    12001 tcttctgccg gcatctcagg tggaaactgt tcatccacat ccattttcca gagctgaaaa
    12061 tcaaagcctg catcacgaag gatctgttgc cttctgggtg atccggaagc aagatatatt
    12121 tgcactttat tcatttgatt atcaggtatt gcacccacaa ggaaagcagg cccggcaaga
    12181 gcatcaactt gctgaccagg cttacaagat gattcagctg cagggtgacc ggtttgctga
    12241 gcacgagcat catccagcac aaaccgaaca tcatcactcc gccaataaca aggaaggatc
    12301 tgtcatcata gaacagccag agatacctgc tggtatatac tgtcagtgct gaaactacca
    12361 caagccaaca aatgaccagt acacgagtgg cctgttcgcc cgccagagca ggaaaagttc
    12421 tgaaccccgc cagcctgtcg ccctccagat cttccagatc ttttaccagt tctctcggaa
    12481 gggtcagcag gaaactgaat actgccaacc ctgccaccca tttccatacg taagccgcag
    12541 tttgatactc agacaggttt tccacttcat aaaaagcagg ttcaaaaact gccggggtaa
    12601 gcacactgat cgctatcaga acagacaccg tcaggttgcc tgccaacgct acccttttga
    12661 gaaaacctgc atataaatag aggagcagca cggccatcac atgcactccg aataaaggta
    12721 gtcgccccac acgcaatgaa atgaacagcc ctgaaatcaa acctgcaatg gtgagaatga
    12781 aatagtatct ccacgcaagg ctttcactca cagaactgcc cacaaactgg cggtctggtt
    12841 tgttgatgcg gtcagcttct acatcgcgaa tgtcattgat gatatagccg gcggctgtga
    12901 tcaggatgac acttattgca agcagactgt actcccagtt ctgcaaatac agagaagggc
    12961 tgccattctt tgttacaggc tgaaacagaa aaaggcggat aaatacgaag gtcagggcta
    13021 caatacccag attaacagga cgaacaatgc gccatgccct gttcatcagg actggttgtc
    13081 acgccattca tacacccagg agctttgaat catctccagg tgtccttcat tgcattcttc
    13141 acgggtacca tgaaacccgt ctatttccag cacccattca atgagttcag tgaatcgtat
    13201 gcggtagatc ttaccttcat tgaaatcatc gccaaaacgc tcatacaatt tgagtgctat
    13261 atcttcatag tcgttccagt gaataggtgg ttcttcaaat accatgtttt attgaattat
    13321 gatgcgatga tggtgctttg atccggaacg atgacctcga tctctccatc cccttccagc
    13381 aggtcgcact ggcaactgag acgtgaatgg agcctgggat ctctggcccg gtcaacgaaa
    13441 tcctcttcct tgtctgtgat ctcttccagc gattccatgc ctttctctat gtaaacatga
    13501 caagtgctgc atgcgcaaac gccgccgcaa ttgtggttga gttctatatc attcagcagg
    13561 gccacttcca gaatggtcct gccggttttg gcttccacgg ttattggctc actaccgtct
    13621 tcaaacacaa acctgatgct gtaatttccc ataatgcggt gcaaagttag tgatgcctgt
    13681 acttttcctg cagatttcgc gacatcaatt cataaacatc ttgcagagca ggatggttct
    13741 gcagcatttg cagatgtttg ctgattactt caagatctct gcgaactgca ggtccggtct
    13801 gtgctgatcc gggatccatt tcttccagtt tgtaaacagt ttcttccagc aacggaagca
    13861 ggtcctggta tgacatgcca gcaccatgca gatactctga agcaagctgt accaggtgat
    13921 tggtaaaatt attgaccacc acagctgcca gatgcagccg cgatcttgta tcatcatcaa
    13981 tgaaacggaa tttcccgttg atcatcagca ccagttgttt caggatcgct tctgtatcag
    14041 gtctgtatgc agtcagaaaa aatggaatgt tgttatatac cggttgacgt tcacgtgaaa
    14101 atgtttgaag cggataacat accccatctg taattccctc tgaaacagca gaaccggaac
    14161 agtgtatcat catacgtcgc tctcctttga gagaacctgc tacttcctga atggcggcgt
    14221 cacttaccgc aaggatcaca aaaagatctt ctgcctgagg cagatgggct ggatccataa
    14281 taccgaatga tcctgccttt gctgccagtt cctgtacgcc atcagtactt ctgcctaaca
    14341 catatgctac ccgcaaacca gccagtgcca gcatatcagg ccagtgccag gccaatctgc
    14401 cagtgcctgc cagtacaatg gtcagatccc ggatgtcttt tgtgtgatcc atatcagccc
    14461 tttcttcgcc ggagatcctc cagacgtttg cgcatgctga tggcaaagcc aatggccagg
    14521 acgatcatac caagccagac cagattgatc atcgggaaca cgatggcttt catgatgatc
    14581 cagtcttgcg gaacattctg ttctctggtc tgaaaaataa aggtatcggt ttccggctgt
    14641 atcatctgaa aaacgatggt tcctccggca tataccaact cagcaggcag ggtacctgtc
    14701 tgactgccgt atatcaggaa ttgcggctcc actactgctg attcattccc gttgagttca
    14761 cggaactgag ccccggctgc aatggtatct cctgtctgat ac
//
LOCUS       DAOMAX010000044        14770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_478_length_14770_cov_4.784370, whole genome shotgun sequence.
ACCESSION   DAOMAX010000044 DAOMAX010000000
VERSION     DAOMAX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14770
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_14770_cov_4.784370"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..822)
                     /locus_tag="PK742_03975"
     CDS             complement(166..822)
                     /locus_tag="PK742_03975"
                     /inference="COORDINATES: protein motif:HMM:NF039623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HQU75778.1"
                     /translation="MDEQLSQWEAAGDRRAIFLACYRTMTANMLAHLEKQFFQDSPWV
                     QSLLDHFADYYFDALLRYENGEETAAVWKQVHDLSKEENISALQRLLMGVNAHINYDL
                     VLSLYDVLHQDWSAWSESERRQKQQDHERVNTVIANTIDVVQDNILEPAQPFMAVVDR
                     AFGRLDEYLLSKLIANWREDVWQHASALLEIQDETRRKTYISELERTVLKRTRMIGMV
                     "
     gene            complement(1126..1626)
                     /locus_tag="PK742_03980"
     CDS             complement(1126..1626)
                     /locus_tag="PK742_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQU75779.1"
                     /translation="MNEEDKRLKKVCIICAKGNLEDVYAALVMANGAVMEGIEAKLFF
                     TFFGLEAITKSHMEHLHTAVVGNPAMRLPGNIPFPSLMGIVPGMEAMASSMMRKKMEE
                     LDIPHVGEFMEMIEAGGGEIFACKLAVDMFGLKKEDLSEHVKDIITIAEFYELAGGDH
                     SQIIFV"
     gene            complement(1645..1959)
                     /locus_tag="PK742_03985"
     CDS             complement(1645..1959)
                     /locus_tag="PK742_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HQU75780.1"
                     /translation="MATKMYAGKPVEVNDEGFFLDKEAWTKDMAPEIAKEVGIDALGD
                     KHYDVIEYIRKRVAAGEQLSMRGMGKSGVVDLKEFYQLFPGKPLKYATRISGVPKPTS
                     CV"
     gene            complement(1962..2660)
                     /locus_tag="PK742_03990"
     CDS             complement(1962..2660)
                     /locus_tag="PK742_03990"
                     /inference="COORDINATES: protein motif:HMM:NF019464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HQU75781.1"
                     /translation="MITETNIDQQLMQDINRKLDLLLEGMQEQQQQKEQVNDLMADAG
                     HIVKDLTQTATLQLDQSGVQVDQELVGEMLLKLLRNLDNINGMLDMLESFSDLSKDAE
                     KIIQNASMDATEKLDALHRKGYFRFAGEMGTVMDRVVEHFSTDDIRDLADNVVNILET
                     VKRITQPDMMEAVNNAIVIFRNVETQDVPEMGLFRVMRELNSREAKKGLGFFVTFLKN
                     LGKQELISHPTQKQ"
     gene            complement(2680..3909)
                     /locus_tag="PK742_03995"
     CDS             complement(2680..3909)
                     /locus_tag="PK742_03995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQU75782.1"
                     /translation="MRRLLILGAGTAGTIMANKLRKDLPTHAWEITIVDEDREHLYQP
                     GLLFIPFGIYKKEDVLKPKANFLPRGVNRIFEGFQKIEPESNKVILNSGKELHYDLLI
                     IATGTRVAPEETPGLKGELWYKDVFDFYTLEGSLALARKLRDWEGGKLVVNIADMPIK
                     CPVAPLEFAFLADWFFQERGIRDKVEIYYVTPMSGAFTKPKASAILSGILEQKHIHVI
                     GDFFLGEVNNEEKKIVDFGGAEVDFDLLVSVPLHMGQQAIIDSGIGDDFGFVPTDKFT
                     LQARDHENIFVLGDASNIPTSKAGSVVHFASEVLHENILCAIDGRPMTASFDGHANCY
                     IETGFGNAALLDFNYDTEPTTGTFPLPGVGPFTLLSESKANHYGKLIFRWIYWHILLK
                     GKELPVSNKMSLAGKDI"
     gene            complement(4108..6279)
                     /locus_tag="PK742_04000"
     CDS             complement(4108..6279)
                     /locus_tag="PK742_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75783.1"
                     /translation="MKKILFTLLAVATFGAASAQSWGYAREGHWAADVAADADGNVFA
                     TGYSWNDPDFLTYNDNYVIKLDADGNELWVNDGLDIPFDYFTSTNVFPDGAGGCTVVG
                     YSNFATAAAYGLDADGNLTWDSEAWTSTSNNNLLYQGASAKLSDGRIVIGGFNEDYFY
                     RFSVVGTDGSLLSEFEIEADTTGWAFSYFSYRETGLCATADGGFAFSAGGEGFFNLMK
                     FDSDLNLDWSADYDHDPDTWEYGNYNNCLSQTSDGGYLLAASGSGGAFGTYMGSVRKM
                     DAAGNLEWTTYLNHGADWEEGSHAMQVGDYYYAWTQDAGDNSSHAWVLDAGGAEIDSV
                     FIPVINCTWGFGETGMEIWAIRPAADGGYYISGRQYVEDCDQRFTVIKSEADGSFGPC
                     IFNCVWPGDANNDGYADADDLFEIGINYGAEGFTRDDDAIDWSAKLARAWMEEDTLYW
                     YVLNDLKYTDCDGNGIINDDDTTAVISNFGLDHPLNTTRTSAEGVPLYFDPSVTTLEV
                     GMNHIPILLGDAIDPVDAIYGIAFTVTTSSSSVDGASLKIRFGDSWMAGADEQLSLSV
                     NSAEEKKAASAMVRTARTNTEGQGEIATLDVVVIDNLAGKLETEDITLHIVDAKAIML
                     DRSEIPVDAQSASFTADISEPSSLNDLEAGGISIYPNPVAAGQQPVVNGSGIEQIEIL
                     DLTGKQVASIRQEPWPVLPAGTYTIRVTNQSGVYTGQLVVL"
     gene            complement(6449..8620)
                     /locus_tag="PK742_04005"
     CDS             complement(6449..8620)
                     /locus_tag="PK742_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75784.1"
                     /translation="MKRLFTFCFLIVVAGATTMAQWSYIQDDPAIQAQANNMAEDAFG
                     NIIAVGGAFGYPLLGDGPPRVTKLDASGNLIWEVELSVPAGIGSISASGVSVVDDGYI
                     IFCGGFEKPLIYKLDPDGAEVWNTELWCSPLALGIATDVRGCVLDDGTIAMFGLDSSV
                     PTNIKIFIVNEDGELISEVTHSTTLDEVYIISAWDLASQGDGFVVTGGGLDATSTWMP
                     YAVKFDAAGEVVWESLFPEYAGLEAYGVCATSDGGYALSAYNFFGGSTAMIKMDGDGN
                     FEWGNTYAATDPGFVAYAYDIAQLADGSYAMLHVNLDTWYLFYGDIEVLLIDETGTET
                     DRITAAAGLSNRANKMIATADGGYAFCGNFTINDYLDPAYDQVYMVQKSSAAGALVDC
                     VYNCVWPGDANNDGLANTDDILAIGVAAGSSGPERDDMSIDWYPHAALSWSDSLAAGD
                     DLKYVDCNGNGTINDDDTAAVALNYSAEHPIYALRTEAGEIELAIDLPETTLTPGYYT
                     FPVLLGDAVNYPDAIYGIRFTVTYEGNDLDLSSVNFLFDDNWFGTAADEIRFRRNDTD
                     LQQLDVALVRKDQNNTSGYGEIGELGFVVIDNIAGRSRSETIVFQITDVRAIDVDMNE
                     INVNGSVAVVDSEGPTGISLQEDVLVRLYPNPVKGSSVMLDLRGLDLPELVTISDLTG
                     RVVSVQQPDSPMLDISMLLAGAYQIQLNWLNNTYMTNLVIE"
     gene            8772..10241
                     /locus_tag="PK742_04010"
     CDS             8772..10241
                     /locus_tag="PK742_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75785.1"
                     /translation="MASPKAIQTFQSLGADRQKRFLLFVQSPYFNTREKLWQLTDYIR
                     HRTEEEEDWDATGASIFIFGDAQQQKPLTNLLSDLNRLLEQFLSTEALLDHEDLRETA
                     LARGMTALSAPRVTQAYLRRKQRLGVQDPLSLHHLNRLADQFHFQQTRQHSNEDLLES
                     QKQLTLFYLTRQLKTWCELLNRRHVLDLPFDPEQLNLFRQILESASDLIDTHATSGMY
                     RAIFIWLSNQESDAWYDRLQADLLDMLAATDADEIREVCAYLQNYFVRRINEGKKRFL
                     QELYNLFQYMLAHDLVLEGQTISQWTYKNIVTVGLRLQYFDTTESFIHEWYSRLPEQD
                     RDNAYYYNLAALHYESRQYDKARKLLQRVHFTDPVYYLDSHCILLKIYWEEGEDEAIR
                     SLHETVRIYLLRQRSLNKQQIQLYRQLFRFTLRLFRLRYTSAHLSATEQQRIYRKIRT
                     DLDLQEEVANRGWLQDQWKLTGGFLKITPSDNEPTEEVE"
     gene            complement(10194..10979)
                     /locus_tag="PK742_04015"
     CDS             complement(10194..10979)
                     /locus_tag="PK742_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU75786.1"
                     /translation="MKKLLQIEWIKLWPYKAFRILFIVYFLLLGLSLLIGRSLNNGEG
                     EELNKLFKILSVPNIWNYYLFVAVLLNIVLGIIVIFVISNEYSFKTMRQNLIDGLNRN
                     QLFVSKLLLISILTFLSTLVVYISGLIAGKVYNPDATFSDLLERNVLIGGYAIACFGT
                     LSMASLFAIILKRSGLATIVFILFIFPLDVILNQGLLKGCCNDYLPVSVLFKGIIEWP
                     GNMIGSFGEEAQTALAWLPVTMGLVYIGLFNFLSWFIIRRRDL"
     gene            complement(10979..11863)
                     /locus_tag="PK742_04020"
     CDS             complement(10979..11863)
                     /locus_tag="PK742_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU75787.1"
                     /translation="METILNTAALTKHYGPITAVNGIDLHVERGSVYGILGPNGSGKT
                     TTLGMLLGAIHPSSGSFSWFGKGTGDENRKRIGAILEHPIFYPYLSGAQNLRITARIK
                     GVPEKDVDRVLQIVELSARKNSPAATYSLGMKQRMAIAAALLGDPEVLVLDEPTNGLD
                     PQGIAEIRGLIREVAASGKTILIASHLLDEVEKVCTHVAVIKNGNLLAQGHVNEILGD
                     EQLLEIASADPAGLKLQLEQLHCSVRSDESGLFLVGLPEGMDASSLNKKLVESGQAIH
                     HLRVRKKKLEESFLEITA"
     gene            11919..12536
                     /locus_tag="PK742_04025"
     CDS             11919..12536
                     /locus_tag="PK742_04025"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQU75788.1"
                     /translation="MLQSHYQDTLTEAGCDEAGRGCLAGPVVAAAVILPKGFYHPDIN
                     DSKQLNEIQRNALVPVIKEHAIAWAISFCTPEEVDALNILRASFVAMHRAVRQLRPPP
                     GLLLIDGNRFYPYPGIPHQCFVKGDARFTPIAAASILAKTERDHLMDILHEQHPEYGW
                     KENKGYPTSVHRDAIRLYGINHHHRKTFRLAPDTQTLLFNESDPV"
     gene            12533..13588
                     /locus_tag="PK742_04030"
     CDS             12533..13588
                     /locus_tag="PK742_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HQU75789.1"
                     /translation="MKRSPLYLLLLLIVTACGGQQHLPPGIHYPATFAAIIPCDDCSG
                     IKYELTLDENMTFTERMIYIGKDDKIYQQNGSWYMVSDSVLLLEKKQGSMNQFLVEQG
                     DLRMLDTHGKRFGGALADRYLLMPGSLSGLISPSSEITSADAGLSDFRATGSDPDWTL
                     EIDFARHIIFRLRGEPEWVMPLPYMHIDSGFQLQYSGQGDRGAMHIVLLPKGCETGFQ
                     QVRVTAGQKTFNGCGQYENQRAQLNGYWVLHELNGKPVESDGGRTIPHCQIDIGAGTI
                     SGHTGCNQFNGPVTITRNTFQPGNLAMTKMACPKSPESALVSLLNVPSQYRFDQGQWQ
                     MIVNGNIVARWIRPDIQ"
     gene            complement(13582..13965)
                     /locus_tag="PK742_04035"
     CDS             complement(13582..13965)
                     /locus_tag="PK742_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQU75790.1"
                     /translation="MRQNYSSGTAWENMIGYSRCVRVGNFIKVSGTTAVNDQGQIMGD
                     SVYEQCHLIYKKIEHALTMAGSSMDHVVMTRIYIRDIQKWEEAGKAHSEVFDEIRPAA
                     TMVEVSGLIHPDMLVEIEVEAFMHY"
     gene            14053..14388
                     /locus_tag="PK742_04040"
     CDS             14053..14388
                     /locus_tag="PK742_04040"
                     /inference="COORDINATES: protein motif:HMM:NF023123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HQU75791.1"
                     /translation="MIISTTELRVKDLLQLPLFFRLSIASVEQARRAPGNLQVSVRGT
                     LLSGMTLTAWKDEASMMAYRNSGAHAEAMRNLKKVTRRYRTIWYEADGIPNWKEARQK
                     LLQKPFREF"
     gene            complement(14415..>14770)
                     /locus_tag="PK742_04045"
     CDS             complement(14415..>14770)
                     /locus_tag="PK742_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75792.1"
                     /translation="LLPSTLYNWRVSAQCTEGWKPFLSLSSFQTLAEREAGQMTENWQ
                     VYPNPAGDVVHMDLPGGTGQISLYDLSGQKLLQLDVPEGSTSLSVPVDHLNRGLYILH
                     WIHTGGQETRQILLQ"
BASE COUNT         3789 a         3832 c         3436 g         3713 t
ORIGIN      
        1 atggcgaaac tgaaaaagct ggcggaagga agttgaaaag cggaatttga aatacggaat
       61 gattggtcag tagtgagccc cgatagcgat cgggaggggt gaatggtgag acctttcatt
      121 gaaaggattt cccaatactc aatacccact actcaatact ctcccctaca ccatcccgat
      181 cattctggtt cttttcagca cggttctttc cagttctgag atatacgttt ttcgtcttgt
      241 ttcgtcctgg atctccagga gtgcagaagc atgttgccag acatcttccc gccagttggc
      301 gatcagtttt gacaggagat actcatccag tcttccgaat gctctgtcaa ccaccgccat
      361 aaaaggctga gcgggttcca gtatattgtc ctgcaccaca tcaatggtat tagcaataac
      421 agtattcacc cgctcatgat cctgttgttt ctgcctgcgt tcagactctg accaggcaga
      481 ccagtcctga tgcaagacat catacaagga gagcaccaga tcatagttga tgtgtgcatt
      541 gactcccatc aataggcgtt gtaatgctga tatattttcc tccttgctca gatcatgcac
      601 ctgcttccag acagcagcag tttcttctcc attttcatat cgcagcaatg catcgaaata
      661 gtaatcagca aaatgatcga ggaggctttg tacccaggga gagtcctgaa agaactgctt
      721 ctccagatgg gccagcatgt tggcggtcat ggtccggtag catgccagaa atatggctcg
      781 tctgtcgccg gcagcttccc attgagacag ctgttcatcc atgtattgca aagtggccat
      841 gagaggtgga gtgcaatata aaaatactga atttgaaata cgggagataa agtagtcaga
      901 agtgagtagt gagtggtgag gtccttcatt caaagaaaat cccaataccc tccctcccga
      961 tagctatcgg gaggggtgag tagtgaaaga acagatccgt tccgacagaa aagatatccc
     1021 aatactaaat actcaatacc cactactcac tcaaagacga atgccggaac aaaagttgcc
     1081 ttctgtcccg gacattcgct tgcggtaccc aaccacagag tagggttata caaagatgat
     1141 ctgagaatgg tcgccaccag ccagttcata gaactcggca atggtgatga tgtcttttac
     1201 atgttcactg aggtcttcct ttttcagacc gaacatatcg accgccagtt tgcaggcgaa
     1261 tatttcgccg ccccctgctt cgatcatctc catgaattct cccacgtggg gaatatccag
     1321 ttcttccatt ttctttcgca tcatgctgct ggccattgct tccattccgg gtacgatacc
     1381 catgagggaa gggaagggaa tgttgccggg caggcgcatg gcgggattac ccaccacggc
     1441 tgtatgcaga tgctccatat gtgatttggt gatggcctcc aggccaaaga aggtgaagaa
     1501 taatttggct tctattcctt ccatgacagc gccattggcc atgaccagcg cagcgtacac
     1561 atcttccaga ttgcccttgg cacagatgat gcatactttt tttagtcttt tatcttcttc
     1621 gttcatgacg tttacttttt ggggtcagac acaactggtg ggtttcggca ctccgctgat
     1681 gcgggtagca tatttgaggg gtttacccgg aaagagctgg tagaattctt tcaggtccac
     1741 cacacctgat tttcccatgc cacgcataga cagttgttcg cctgcagcta cccgcttcct
     1801 gatgtactcg atcacgtcat agtgcttgtc gcccagggca tcaatgccta cttcctttgc
     1861 gatctcagga gccatgtctt tggtccatgc ttctttgtca agaaagaaac cttcatcgtt
     1921 tacttctacc ggtttaccgg catacatttt cgttgccata attattgttt ttgggttggg
     1981 tgagaaatca attcctgttt gcccaggttc ttgaggaagg tcacgaagaa gcccaggcct
     2041 ttcttggctt cgcgactgtt gagttcacgc atgacccgga agagtcccat ttcgggcaca
     2101 tcctgggtct cgacattgcg gaagatgaca atggcattgt tcaccgcttc catcatatca
     2161 ggttgcgtga tgcgtttcac ggtttcgaga atgttgacca cattatctgc cagatcgcgg
     2221 atgtcatcgg tgctgaagtg ttccaccacc ctgtccatca cagtgcccat ttcgcccgca
     2281 aaccggaagt atccttttcg gtgcagtgca tccagttttt cagtagcgtc catactggca
     2341 ttctgaatga tcttctctgc gtctttgctg aggtcagaaa agctttccag catgtccagc
     2401 ataccgttaa tattatccag gttgcgcagc agtttgagca gcatttcgcc cacgagttcc
     2461 tgatctacct gcacacctga ctgatccagc tgtagggtag cagtctgtgt aaggtctttt
     2521 acaatgtggc ctgcatcggc catcaggtca ttcacttgtt ccttttgctg ctgctgttcc
     2581 tgcatgccct ccagcagcag atccagcttg cggttgatgt cttgcatcaa ctgttgatct
     2641 atgttggttt cagtgatcat ggttgggtcg tttattcgtt cagatatctt ttcctgccag
     2701 actcattttg ttgcttaccg gcagttcctt gcctttgagc agtatatgcc agtagatcca
     2761 gcggaagatg agcttgccat agtgattggc cttgctttca ctcagcaggg tgaaaggacc
     2821 tacacccggc agcggaaaag ttcctgttgt tggttcggta tcgtagttga aatcgagcag
     2881 ggcggcattg ccaaatcctg tttcgatata gcagttggca tgtccgtcaa agctggcagt
     2941 cataggtctg ccgtctatgg cacagagtat attctcatgc agcacttcgg aagcgaaatg
     3001 caccacactg cctgctttgg aagtaggtat gttggaggca tcacccagca caaagatgtt
     3061 ttcatgatct ctcgcctgca gggtgaattt atctgtgggt acaaagccga aatcatcgcc
     3121 tatgccactg tcaatgatcg cctgctgacc catgtgcaat ggtacactta ccagcagatc
     3181 gaagtccact tcggcaccac caaagtccac gatcttcttc tcttcattat tcacttcacc
     3241 caggaaaaaa tcgcctatca catggatgtg cttctgttcc agaatgccac tgagaatggc
     3301 agaggctttg ggtttggtga aggctcctga catgggggtc acatagtaga tctctacttt
     3361 atcgcgaatg cctctttcct ggaagaacca gtcggccaga aaagcgaatt ccagaggtgc
     3421 taccggacat ttgatcggca tgtcagcgat gttgaccacc agtttgcctc cttcccagtc
     3481 ccggagcttc ctggctaatg cgagtgatcc ttccagtgta taaaaatcaa atacatcttt
     3541 ataccacaat tcacctttca gaccgggagt ttcttccgga gccaccctgg taccggtagc
     3601 aatgatcaga agatcatagt ggagttcttt gccgctgttg agtatgacct tgttactctc
     3661 gggttctatc ttctgaaatc cttcaaaaat gcggttgacg cccctgggca gaaaattagc
     3721 tttcggcttc aggacgtctt ctttcttata aatgccaaag ggaatgaaca acaggccggg
     3781 ttgatacaga tgttcccggt cttcatccac tatggtgatt tcccacgcat gtgtggggag
     3841 gtctttcctc agtttattgg ccatgatggt acccgcggta ccggctccta atatcaataa
     3901 ccttctcatg gttgggtagt gctttgatta cactacaaag caactccagc cacgaaacag
     3961 gctgtgcaac cgtggttaca caagaaaatg acagatatca ccaaaataaa aaccccgtcc
     4021 cggaggagtg tgggacgggg tttgaaccga tcttaacctt aactatctgc aatagtgcaa
     4081 accgtatgcc agagaacggc ccactcatca aagtacgacc agctgaccag tatatacacc
     4141 ggactggttg gtcacacgaa tggtatatgt accggcaggt aaaacgggcc atggctcctg
     4201 gcggatgctg gccacctgct tgcctgtcag atcaagaatc tcaatttgct ctataccact
     4261 accattcact accggttgct gaccggcagc aacagggttg ggatagatgc tgatgccacc
     4321 tgcttccaga tcattcagag aagaaggttc gctgatatca gcagtgaagg aggcagattg
     4381 tgcatctacc ggtatctcgc tgcgatccag catgattgcc ttggcatcaa cgatatgcag
     4441 ggtgatgtct tccgtttcca gttttccggc caggttatcg atgaccacta catccagtgt
     4501 agcgatctca ccttgtcctt cagtattggt ccgggcagtg cgcaccatgg cagaggcagc
     4561 tttcttttct tcggcactgt tcacactcag tgacagctgt tcatcggcac cggccatcca
     4621 gctgtcgcca aagcggatct tcaaagatgc accatccaca cttgaactgg atgtggtcac
     4681 ggtgaaggcg attccataaa tggcatcaac cggatcaatg gcatcaccca gcaggatcgg
     4741 aatatgattc atacccactt cgagggtggt tacagaagga tcgaaataca ggggaacccc
     4801 ttctgcagag gtgcgggtgg tattaagcgg atgatccagg ccgaagttgc tgatcacagc
     4861 ggtggtatcg tcgtcgttga tgataccgtt gccatcgcag tcagtgtatt tcaggtcgtt
     4921 gagtacatac cagtaaaggg tatcttcttc catccaggca cgggccagct tggcactcca
     4981 gtcgatggca tcatcgtctc tggtaaaacc ttctgcacca taattgatac caatttcgaa
     5041 caggtcatct gcatcagcat agccgtcgtt gttggcatcg ccgggccaca cacagttgaa
     5101 gatacaggga ccgaaacttc cgtctgcttc agatttgatg acggtgaacc gctgatcgca
     5161 gtcttccaca tactgacgtc cggagatata atagccacca tcggcagcag gtcgaatagc
     5221 ccaaatttcc ataccagttt cgccaaagcc ccaggtgcag ttgatgacag gtatgaatac
     5281 gctgtctatt tcggcaccac cggcatccag tacccaggcg tgcgaactgt tatcgccggc
     5341 atcctgtgtc caggcgtagt aataatcgcc tacctgcatg gcatggctgc cttcttccca
     5401 gtctgcaccg tgattcaggt aagtggtcca ttccaggttt ccggctgcat ccatcttgcg
     5461 aacgcttccc atataggtac caaaagctcc gccactgcct gaggcagcca gcagataccc
     5521 gccatctgag gtttggctca gacagttgtt gtaattgcca tactcccagg tatcgggatc
     5581 gtgatcataa tcagcactcc agtccaggtt caggtcactg tcaaacttca tcaggttgaa
     5641 gaaaccttca ccacctgctg aaaatgcgaa gccgccatct gctgtggcac agaggccggt
     5701 ctcacgataa gagaagtagg agaaagccca tccggtggta tcagcttcta tctcaaattc
     5761 agacagcagg ctgccgtctg ttcctactac tgaaaagcgg tagaagtaat cttcgttgaa
     5821 gccacctatg acgatacggc catcactgag ttttgcagag gcaccctgat acagcaggtt
     5881 gttgttgctg gtactggtcc atgcttcaga atcccaggta aggtttccat ctgcatccag
     5941 accataggca gcagcggttg caaagttgga ataaccaaca acggtacaac cgccggctcc
     6001 gtcaggaaac acattggtag aagtgaagta atcaaatggt atgtccaggc catcattcac
     6061 ccacaattca ttaccatcgg catccagttt aattacatag ttgtcgttat aggtaagaaa
     6121 atcagggtca ttccaggaat aaccggtagc aaaaacattg ccatcagcat ctgcggcaac
     6181 gtctgctgcc cagtgacctt cgcgggcata accccaggac tgggctgaag cagcgccaaa
     6241 ggtggcgact gccagtaagg taaacaggat cttcttcata ggctgctatt tgtgtggata
     6301 caattccacg ttacaaatct gcaagaccag ctgcacaaaa atatgtacaa aaaatagtgt
     6361 ttacaggtgc tgtttgataa aataaaaaac acaaagtgct taataacagc actttgtgtt
     6421 tagcagtcca attgaaaaac agaaaattct attctattac cagattggtc atataagtat
     6481 tgttcaacca gttcagttgt atctggtagg cacctgccaa cagcatgctg atatccaaca
     6541 tgggcgaatc cggttgctga acgcttacca ccctgccggt aaggtcagag atggtgacca
     6601 gttcaggcag atccaaacca cgcagatcca gcattactga tgaacccttt accggattag
     6661 gatagagccg aacaagcacg tcttcttgca ggctgatgcc tgtggggcct tctgaatcta
     6721 caaccgctac actgccgttc acattgatct cattcatatc tacatcaata gcacgtacat
     6781 cggtgatttg gaatacgatg gtctcgctgc ggctgcggcc tgcaatatta tcgatgacca
     6841 cgaaaccgag ttctccgatc tcgccatagc cgctggtatt gttctggtct tttcgaacca
     6901 gtgctacgtc cagttgctgc aggtctgtat catttctgcg aaagcggatc tcatctgccg
     6961 cggtaccgaa ccagttatca tcaaacagga agttgacaga agaaagatcc aggtcattac
     7021 cctcataggt aacagtgaaa cgaataccat agatggcatc aggataatta acggcatcac
     7081 ccagcaacac cgggaaggta taataaccgg gtgtcagtgt ggtttccggc aggtcgatgg
     7141 caagttcaat ttcgcctgct tcggtgcgca gtgcatatat gggatgttct gcactatagt
     7201 tcagagcaac cgctgccgta tcatcatcgt tgatggtacc attgccgtta caatcaacat
     7261 acttcagatc atcacctgcc gcaagagaat cagaccagct gagagcagca tgcggatacc
     7321 agtcgatgga catatcgtcc cgctcaggac cggagcttcc tgcagccaca ccaatggcca
     7381 ggatatcatc tgtgttggct aagccatcat tattggcgtc gccgggccag acgcagttgt
     7441 acacgcagtc aacaagggcg cctgcagctg atgatttctg caccatatac acctggtcat
     7501 aggcaggatc cagataatcg ttgatggtga agttgccaca gaaggcataa cctccgtctg
     7561 cagttgcgat cattttgtta gccctgttac taagacctgc cgcagctgtt atcctatcag
     7621 tttctgttcc ggtttcgtct atcagtaaga cttcaatgtc gccataaaac agataccagg
     7681 tatccagatt cacgtgcagc atggcatagc tgccatcagc cagctgtgct atatcatagg
     7741 cataagcaac aaaacccggg tcggtcgcgg cataggtatt tccccattca aagttgccat
     7801 cgccgtccat tttgatcatg gcagtagagc cgccaaagaa gttataggca gacaatgcat
     7861 agccaccatc ggaggtagca cagactccat acgcttccag gcctgcatat tccgggaaca
     7921 gactttccca gaccacctcg cctgcagcat caaacttcac tgcataaggc atccaggtgg
     7981 atgtagcatc cagcccacct cctgttacca cgaagccatc gccctgagaa gcgagatccc
     8041 atgcagaaat gatatatact tcatccagcg tggtggaatg ggtcacctca ctgatcagtt
     8101 cgccatcctc atttacgatg aagatcttga tgttggtagg aacggatgaa tccagcccga
     8161 acatagcgat ggtgccatcg tccagcacgc aacctctcac atcagttgca atacccaaag
     8221 ccagtgggct gcaccataat tccgtgttcc atacctcagc accatccggg tccagtttgt
     8281 agatgagtgg tttttcaaag ccaccacaaa agatgatata accatcgtcc actactgata
     8341 ccccactggc agagatggaa cctatgcctg ccggtacgct gagttccact tcccagatca
     8401 gattgccgct ggcatctagc ttggtgaccc gcggcggacc atcacccaac agcgggtagc
     8461 cgaaagcccc tcccaccgct atgatattgc cgaaagcgtc ttcagccatg ttattagcct
     8521 gggcctgaat tgccggatcg tcctgaatat atgaccactg ggccatggtt gttgcacctg
     8581 caacaacgat cagaaagcag aatgtaaaga gtcgtttcat aattattcca tttaacgtta
     8641 gcaaaaatgc ctggttttca ccattgacaa tcatactttt caatccattt acagggtaaa
     8701 tattgtaaat actgcatgag atatcactga ttttcaacga gttttatcta tacttacaga
     8761 taatttacag aatggcaagt cctaaagcca tacaaacctt tcagtcattg ggtgccgacc
     8821 ggcaaaaacg attcctgctg tttgtgcaga gcccgtattt caatacccgg gaaaaactgt
     8881 ggcagctgac tgattatatc cgtcatcgga cagaagagga ggaagattgg gatgccacgg
     8941 gggcttccat attcattttt ggtgatgcac aacagcaaaa accgctcacc aatttattga
     9001 gtgatctgaa tcgattgctg gaacagttcc tgtcaaccga ggccctgctt gatcatgaag
     9061 accttcgtga aacagcactg gccagaggca tgacagcact cagtgcacca cgggtaacac
     9121 aagcttatct gcgccgcaaa caaaggcttg gggtacagga tccgttgtct cttcatcacc
     9181 tgaaccggct ggcagaccag ttccattttc agcaaacaag acaacacagc aatgaagacc
     9241 tgctggaaag tcagaaacag ctgacattgt tctacctgac ccgccagctc aagacctggt
     9301 gcgaactgct caaccgccgg catgtgctgg atcttccttt tgatccggaa cagctcaacc
     9361 tgttcagaca gatcctggaa agcgcctctg atcttattga cacccatgcc accagcggca
     9421 tgtaccgggc catcttcatc tggctgagca accaggagag cgatgcctgg tatgatcggt
     9481 tacaggcaga cctgctggat atgctggctg ctaccgatgc cgatgagatc agggaggtgt
     9541 gtgcctatct gcaaaactat tttgtgcgcc gaatcaatga ggggaagaaa agattcctgc
     9601 aggaactgta taatctgttc cagtatatgc tggcgcatga tctggtactg gaagggcaga
     9661 ccatcagtca gtggacctat aagaatatag tgaccgtagg tttgcgtttg cagtatttcg
     9721 atacaacaga gtcgttcatt catgagtggt acagtcgcct gccggaacag gaccgtgaca
     9781 atgcctacta ttacaacctg gcagccttgc attatgagag ccgccagtat gataaagcca
     9841 ggaagttgct gcaaagggtg cactttaccg atccggtcta ttacctggac agccactgca
     9901 tcctgctgaa gatctactgg gaggaaggcg aagacgaagc catccgttca cttcatgaga
     9961 ccgtaaggat ctatctgctc aggcagcgca gtctgaacaa gcaacagata cagttgtacc
    10021 ggcagctgtt ccggttcaca ctcagattgt tccggttgcg ctatacatct gctcatttat
    10081 cagcaactga acaacaacgc atctaccgca agatcagaac cgacctggat cttcaggaag
    10141 aggtggccaa ccggggctgg ctgcaggatc agtggaagct tactggagga ttcctaaaga
    10201 tcacgccttc tgataatgaa ccaactgagg aagttgaata acccgatata caccaacccc
    10261 atagtgaccg gcagccaggc cagtgcggtt tgcgcttctt caccaaagct tccgatcatg
    10321 tttcccggcc actcgatgat acccttgaat aagacactga caggcaggta gtcattacag
    10381 caacccttga gtaatccctg attcagaata acatccaacg gaaagatgaa caaaatgaaa
    10441 acaattgttg ccaaccctga acgcttcagg atgatagcaa ataatgacgc catggaaagg
    10501 gttccgaaac aggcaatggc atacccgcca atcaggacat tgcgttccag caggtcgctg
    10561 aacgttgcat caggattata cacctttcct gcaatcagtc cggatatata aaccaccagt
    10621 gttgacagaa atgtgagaat ggaaatgagc agtaacttgc tgacaaacag ctggttcctg
    10681 ttcagcccgt ctatcagatt ctggcgcatc gtcttgaagc tgtactcgtt actgatcacg
    10741 aaaatgacaa tgatacccag tacgatgttc agcaatacag ccacaaacag atagtaattc
    10801 catatatttg gaacagataa tattttgaac agcttgttca gctcctcacc ttctccgttg
    10861 ttcaggctcc tgcctatgag cagactcaga ccgagcaaca ggaaatatac gatgaacagg
    10921 atgcgaaaag ccttgtaagg ccatagcttg atccattcaa tttgcagcag tttcttcatc
    10981 aggctgtgat ttccaggaat gactcttcca gtttcttttt acgtactctg aggtgatgaa
    11041 tggcttgtcc ggactccacc agttttttat tcagcgaaga ggcgtccata ccctccggca
    11101 aaccaaccag aaaaagaccg ctctcatcag aacgaaccga gcaatgtagt tgctccagct
    11161 gtagcttcag ccctgccggg tcagcagatg cgatctccag cagttgttca tctcccagta
    11221 tctcattgac atgcccctgt gccagcagat ttccattctt aatgaccgcc acatgggtac
    11281 atactttttc cacttcatca agcaggtggc tggctatcag aatggtcttg ccggaggcag
    11341 ctacctcgcg gatcagccct ctgatctctg ctataccctg cggatcaagc ccgttggtgg
    11401 gttcgtccag taccagtact tccgggtcgc ccagcaatgc tgctgcaatg gccatacgtt
    11461 gtttcatgcc cagtgaatag gtagcagcag ggctgttctt tctggcactg agctctacga
    11521 tctgcaaaac cctgtccaca tctttttcgg gcacaccctt gatcctggcg gtgatgcgca
    11581 ggttctgtgc tccactcaga tagggataga aaataggatg ttccagtata gcgccaatgc
    11641 gttttctgtt ctcatctcct gttccttttc caaaccagga aaaacttcct gaactgggat
    11701 gaatggcacc gagcagcatc ccaagggtag tggtcttgcc actgccgttc ggacccagaa
    11761 ttccatatac agacccccgc tctacatgca ggtcaatacc attcacagca gtgatagggc
    11821 cgtagtgttt ggtaagtgcc gcggtgttca agattgtttc caattgcacg cttttgggta
    11881 aaactactaa tttcactctg acgcatcaca cgttaaaaat gttacagtcg cactaccagg
    11941 ataccctaac agaagccggc tgcgatgaag caggtcgcgg ttgtctggcc gggccggtag
    12001 tagctgctgc cgttatcctc ccgaaagggt tttaccatcc tgacatcaac gacagcaagc
    12061 aactgaacga gatacaacgc aatgctctgg ttcctgttat caaggaacat gccatagcct
    12121 gggccatctc tttttgtacc ccggaagaag tagatgcact caatattctt cgtgcctctt
    12181 ttgtggccat gcacagggct gtcagacagc ttcgcccgcc ccccggtctg ctcctgatag
    12241 acgggaaccg tttttatcct taccccggta ttccgcacca atgctttgtt aaaggtgatg
    12301 cccgctttac acctatagct gccgccagta ttcttgcgaa gacagaaaga gatcatctca
    12361 tggacattct gcatgaacaa cacccggaat atgggtggaa agaaaacaag ggctatccta
    12421 ccagtgtaca tcgtgatgcc atcagactgt atggcatcaa ccaccaccac cggaagactt
    12481 tccggctggc gcccgatact cagaccttat tgttcaatga atcagatccg gtatgaaaag
    12541 atccccctta tacctgttac tgctcctgat cgtcactgcc tgcggtggcc agcagcatct
    12601 tcctccggga attcactatc cggccacctt tgctgccatc attccctgcg atgattgctc
    12661 cggcatcaaa tatgaactga ccctggacga aaacatgacc ttcacggaac ggatgatata
    12721 tattggaaaa gatgacaaga tctatcagca aaacggctcc tggtatatgg tcagcgatag
    12781 tgtgctcctc ctggagaaaa aacagggtag tatgaaccag ttcctggttg aacagggcga
    12841 tctgcgtatg ctggatacac acggcaagcg ttttggcggg gctctggccg atcgttacct
    12901 cctgatgccc ggttcactta gcggactgat ctcaccatcc tcagagatca cttcagctga
    12961 cgccgggctc agcgacttcc gtgccacagg ctcagacccg gactggaccc tggaaattga
    13021 ctttgcccgc cacattatct tccgccttcg cggggaacct gaatgggtca tgccgctacc
    13081 ttatatgcat atagattcag gctttcagct gcagtacagc ggacaaggcg accgcggcgc
    13141 catgcacatc gtgttgctgc caaaaggctg tgagaccggt tttcagcagg tcagggtaac
    13201 tgccggtcaa aaaaccttca atgggtgcgg tcagtacgaa aatcaaagag cacaattaaa
    13261 cggttactgg gtacttcatg aactgaacgg caaaccggtg gagagtgatg gtggccgcac
    13321 cataccgcat tgccagatcg acattggcgc cggtacaatt tccggtcata ccggctgcaa
    13381 ccaattcaac gggcccgtca ctataacacg aaacaccttt cagccgggca atctggccat
    13441 gaccaaaatg gcctgtccga aaagtcctga atcagcactt gtcagtttgc tgaatgtgcc
    13501 ttcacagtac agatttgacc agggtcagtg gcagatgatc gtaaatggca acatcgttgc
    13561 acgttggata agacctgata ttcaatagtg catgaaggct tccacctcga tctccaccag
    13621 catatcagga tggatcaacc cagacacttc taccatggta gcagccggcc gtatctcatc
    13681 aaacacttca gaatgagcct ttcctgcttc ctcccacttc tgaatgtcac gaatataaat
    13741 tctcgtcatg accacatggt ccatggacga accggccatg gtcagtgcgt gctcaatttt
    13801 tttatagatc agatgacatt gctcatagac cgaatcgccc atgatctgac cctgatcatt
    13861 gaccgctgtg gtaccgctta ccttaataaa attgcctacc cgcacacaac gggaataacc
    13921 tatcatattt tcccaggctg tgcccgaact gtaattctgc ctcataagat ccggtttatt
    13981 ttcttgtacg tacaaaatta gaaggggttt tggtgatact tgtcaataca ttcattaaac
    14041 atgcaacaat aaatgatcat cagtactaca gaactccgtg taaaagatct gcttcagttg
    14101 ccccttttct ttcgtctgag catagcctct gtagaacagg ccagacgggc tccgggaaac
    14161 ctgcaggtct cagtcagagg aaccctcctg tctggtatga ccctgaccgc ctggaaagac
    14221 gaagcaagta tgatggcata ccgcaacagc ggtgcccatg cagaagctat gcgcaatttg
    14281 aaaaaagtta cccgcagata ccgaaccata tggtatgaag cagacggcat accaaactgg
    14341 aaagaagccc ggcaaaaatt gctgcaaaaa cctttcagag agttttaaaa aaaaaggggg
    14401 caatgccccc gtgtttactg cagtaatatc tggcgggttt cctgaccacc ggtatggatc
    14461 cagtgcagaa tatataaacc cctgttcagg tgatctaccg gtacagaaag actggtgctt
    14521 ccttcaggca catccagttg caacaacttt tgtccgctaa gatcatacaa actgatctgg
    14581 cccgtacctc ccggcagatc catatgcaca acatcgcctg caggatttgg atatacctgc
    14641 cagttttcag tcatctgacc tgcctctctt tctgcaagcg tttggaaaga tgaaagactt
    14701 aagaatggct tccacccctc agtacactga gcactcaccc tccaattgta caaagtggaa
    14761 ggcaacaggg
//
LOCUS       DAOMAX010000045        14286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_498_length_14286_cov_4.067037, whole genome shotgun sequence.
ACCESSION   DAOMAX010000045 DAOMAX010000000
VERSION     DAOMAX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_14286_cov_4.067037"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PK742_04050"
     CDS             <1..832
                     /locus_tag="PK742_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75793.1"
                     /translation="NQKQNILNAINNSVSINNFKKEYESRWAGQVKTANLLFPTIRNN
                     VSQKSIKTFEWGDKWPEDAWLFADISDGGFIHCEKIEVQEGCVLIGAQFIKFGNRLAV
                     QASQAEIATNLAVPVEAGSIVNGLPSVAGPHNWDIPGKNDPDGFFEAPKGIRWNTDYI
                     DLPDDYVATGIKIYCINNVVGIKLKATKLLGDVNTEDIKLSTRAADTAWFPDDAGAGS
                     PSSSLTIDNTPPNHTGAWADMHPVVADPPAYIVGGGVYQYGNRVAILLTPASIEILNP
                     "
     gene            complement(913..1584)
                     /locus_tag="PK742_04055"
     CDS             complement(913..1584)
                     /locus_tag="PK742_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75794.1"
                     /translation="MKAIPVSILILLSAAWTSQLFSQEVIICPDQPIPPGWVVIDVET
                     CAGCCTPGQLDYRPVIRKVDTLAPETELTVCPQPMPDGWVITDYKTCAGCCGQPGQLV
                     YQPVIKKTDHLPAGTELTVCPQTLPWGWVITDMTSCAGCCAQPGQLVYQPEITRIDLY
                     PLGTRVEICPDQDIPPGWVVVKTSTCAGCCGQPGKLVYRQVIEKIEEIRPVYRLRYFK
                     PEQQD"
     gene            complement(1605..3752)
                     /locus_tag="PK742_04060"
     CDS             complement(1605..3752)
                     /locus_tag="PK742_04060"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQU75795.1"
                     /translation="MKMNKLFTLIIIGSLTSSASAQEGMWIPSLLETNEDEMHAMGFQ
                     LDAEDVYSINEGSMKDAVLIFGGGCTGEVISDKGLVLTNHHCGFDAVNQLSSVDHNYL
                     RDGYWAENMAGELPGPGLKVTFIVRMEDVTSSILKGLDPSANNAVIIQQRSDSILVAA
                     TKDSHYGGYVRPFYYGNQYFLFVTEEFTDIRLVGVPPRSVGEFGGETDNWIWPRHTGD
                     FSIWRIYAGPDNKPAAYSPDNKPFVPRYHFPVSLAGAKEGDFTMVFGFPGRTQEYLPA
                     AAVELIMNEQNPNRVALRELRIRTAESFMHGNDTITLMYASKVKSLANAYKKWQGETL
                     GLTANNAIDKKLEFEYRFDDWCSTPAGKPYAGVTDELDSLYRALNAYTGFLDHTIEGA
                     LGVEAIRFARSFRGLATRLNEEEPDKDRIAQAIDDMRNTAASHFSNYYHPIDEAVFPL
                     LMKRVYEQVDTALLPDYFLEQIVRYKGDFVTWAESLWNSSALVSLEGTNNLLDRIAKG
                     EKDALANDPVYMLYDAFLQQYLLTNNHSVQPLQEAIDRKMQLYMAGIMASGLKDRLYP
                     DANSTLRVAYGQVNGYTPRNGVYYKPYTTHEGILEKYKDGDEEFDVPDQLLALLQGND
                     FGRYADANGDLPVAFLASNHTTGGNSGSPVLNANGELIGTNFDRVWEGTMSDIMYDFD
                     RCRNISVDIRYTMFITEYLGGARWVIDEILQTK"
     gene            complement(3792..4658)
                     /locus_tag="PK742_04065"
     CDS             complement(3792..4658)
                     /locus_tag="PK742_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HQU75796.1"
                     /translation="MVLYNPKDWWKLIFAFRKSDTFRRSLPGILGVAAYATATVWVEK
                     EILHLEFVNTTVIHSLVGFVLSLLLVFRTNSAYDRWWEGRKIWGSFVNNSRNLALKVS
                     VLMQSKELRERFRILIPNYIYAAKEHLRGPVDIRELEPCDKYPVTYYNRQGHVPSQVM
                     KAMYSEIEVWKQSENPEPERLLYLNNELQSFTDNIGACERIKKTPIPYSYNIFLKKVI
                     FLYIFTMPIGFAQQFSYWTALIVPLLFYIFISIEMIAEEIEDPFGKDENDLPTDAIYE
                     TIRENVDEIIGR"
     gene            complement(4820..7636)
                     /locus_tag="PK742_04070"
     CDS             complement(4820..7636)
                     /locus_tag="PK742_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75797.1"
                     /translation="MRIPVLSILLFWSVYLFSQQQIVEIHDNLEIHNLDEKMLVLVDS
                     GCTLTIEDILAGHTPTGFQPLTDSVGKSDTPVAYWLTFRLTSAEDINHQAVIIKNPDY
                     QNSVNRSVFTGGNEFADAYFLDGSGKMTRVSDGFLVPAKDRPLYSHPLMNRMPFTLSA
                     GDTLTVYVRLFNQSDFTTGLSAEVRPDAIALPAEMEGLSVLMGISMGVTGILAILVFM
                     FFLVDRDKANLFFSLLCVTLMLHYSIIHNDLWFVRWFIPNRPWLAEYVWIFFTTFGEL
                     FFLQFGRHFVNLPKVAPRIDKYVRWASWLIVLQFLLDTTLAVRHSGTDLTLASGILLV
                     AILALCIRMAFLPGRLPKIFAVGAVWLLFFNCLGILYSAEYFQLFNPWPVGQMGLMII
                     YSYGLAYKLQLTERTRSDAERILELDAVKSRFFANISHEFRTPLALILGPLRKAQEQA
                     PVSENDSATTDVDIPVSSRHLQMMRRNAERLNQLIDQLLDLSKLENGSMQLQVARADL
                     SAFLRAIVGSFDSLAEIKQIHFQTSYPQADMKVFFDADKLEKIIVNLLSNAFKFTPER
                     GRVSVDSTIEKGRLKIRVSDSGPGISQDEVDRIFERFYQVEGTEDTGTGIGLSLVKEL
                     VELHHGQITVESSRQGTTFKVSLPVDQENFHNTEIVAAAKTPVNRHTLHFLPDAEEPV
                     DSIAEITDNPLPLLLIVEDNTDLRSFIAEGLEEHYRIIKAENGALGLTAALEYTPDII
                     ISDVMMPEMDGMTLCKKLKEDEHTSHIPVILLTAKAGKENRLTGLETGADDYLTKPFD
                     QDELRVRAGNLVAQRKKLRERFSSNHVWKPKELAVTSADERFLQKVSDIIEKQMDNEF
                     FSVEDLADAVAFSRSQLHRKLKALVDKSPSEIIRSFRLTRAKELLEKGDGNVSEVAIK
                     VGYGSLSYFTKSFKAEFGVLPSEI"
     gene            complement(7705..8253)
                     /locus_tag="PK742_04075"
     CDS             complement(7705..8253)
                     /locus_tag="PK742_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HQU75798.1"
                     /translation="MKTIRIMMLGIAVLFFHHVSIAQNRSQDEKNINQILSDMEKAWN
                     TKNGQLWASHMAEQHDWTIWFGMFLPDMDRETNANTHQGLFDTQFQHTNLHMHMTRIR
                     FLSDDIAIANYLANTYETGTKEKNWPEMVGSMVVQRTANGWEVISFGNQDIEYNEILK
                     TNEPSAEAIEGFARNQFRQWYQ"
     gene            complement(8285..8887)
                     /locus_tag="PK742_04080"
     CDS             complement(8285..8887)
                     /locus_tag="PK742_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75799.1"
                     /translation="MEKQTLQKQVWNTSLVLAPILLAAAQFFWDDGRVTTTAGVLQVL
                     AFTVWIFSFQGLFQVIRKDLPTYSVIGFILAAYACIGGNNFGVDGIYTEAMGFTDLEA
                     AAEFTGSIGSSTLAFLFIPGGLFPLSMMILSVQLTRKKKIRLLTGVLFFIGALCFPLS
                     RIPRIDMLAHIDNLILLIAHLLIYTDLKAGTENVGQLQTA"
     gene            complement(8910..9092)
                     /locus_tag="PK742_04085"
     CDS             complement(8910..9092)
                     /locus_tag="PK742_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75800.1"
                     /translation="MSHKPVSIIHRLSRMLPIALLLVAGYTVGIMSRTIYQDLSGTFQ
                     KHYSAFFTEISNYYSK"
     gene            complement(9448..10707)
                     /locus_tag="PK742_04090"
     CDS             complement(9448..10707)
                     /locus_tag="PK742_04090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQU75801.1"
                     /translation="MASTKLLIIGGGTAGIMVAAKFRKEHKNVDVTLLEPGDTHYYQP
                     AWTLVGAGTFDFKKTARSMSSVMPKGVNWVKDKATGFDPENNLVHTEKNGDIPYDYLV
                     VATGLVMEPTMIEGLAEALDKGTVVSNYTNPEYTWEVIRNFKGGNAVFTQPTTPIKCG
                     GAPQKIAYLAADYFRKKGIADKSNVIFATPGTVIFGVKPIAHTLMEVINRYDIHFKPF
                     YAPHRIDSENKIIHFKHSAPEENRCVINEDNSLGETMVGDTDISIPYDMLHLAPPQAA
                     PRFVRESRLANAQGWLDVNINTLQHNRYPNVFGLGDVAALPTAKTGAAIRKQVPVVVN
                     NLVHLMQENKLGSKEYNGYSSCPLVTGYGKMVLAEFDYQNNFTPDPKLKQMLVFNSDK
                     EHWRLWMLKKYGLPYLYWNKMLKGVDV"
     gene            complement(10904..11611)
                     /locus_tag="PK742_04095"
     CDS             complement(10904..11611)
                     /locus_tag="PK742_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75802.1"
                     /translation="MIRIFRNLRSRLIGGGAFRKYLLYAIGEIVLVMIGILLALRVNN
                     NNIARVEHERETKYLQNIRLDLDKDVENLTFLIDFRADRIQSLDRLITYCDGIQPQDP
                     DSLALDVIMSLYEYWFNPSNVTVKDLLGSGNMNLIRNASIKEKLFDLELLYQSNTSNL
                     EHETYEYQQYLSKPIFTHADVDKMAQIFLKEHTAGELGLTVANFEGLLHDPVYRNGCA
                     IASLTSLEYSDLFRQIL"
     gene            complement(11639..11992)
                     /locus_tag="PK742_04100"
     CDS             complement(11639..11992)
                     /locus_tag="PK742_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HQU75803.1"
                     /translation="MSNNRVFQMSFAGVYPHYITKAEKKGRTKEEVDAVICWLTGYDQ
                     KGLQEQIDQKTSLEQFFGQAPQIHPNASLITGVICGYRVEDIEDPLMQKIRYMDKLVD
                     ELAKGKKMEKILRES"
     gene            complement(11999..12532)
                     /locus_tag="PK742_04105"
     CDS             complement(11999..12532)
                     /locus_tag="PK742_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75804.1"
                     /translation="MSKAVIILVLSYSIVQAQQPADTTLLPSVCYYDEPVNDYLREEL
                     RPARDYVYYLTCQELMPVISATAQIDSLNATIYYLYNSWDLQWIIMKWGSYPAVRMTE
                     FFFRENDMILCRERTLVPEEGISDVRSYFRNGKLLHQAINFDCGAPFADDHLVTEQER
                     IQQLYLSLISIQPEPNE"
     gene            complement(12547..12765)
                     /locus_tag="PK742_04110"
     CDS             complement(12547..12765)
                     /locus_tag="PK742_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HQU75805.1"
                     /translation="MSFQAYIDNIKAKTGKSPADFKKLAEQKEFLIDGKLNPDVKATQ
                     ITNWLKEEFGLGHGHAMAIYATFKGKTK"
     gene            13214..>14286
                     /locus_tag="PK742_04115"
     CDS             13214..>14286
                     /locus_tag="PK742_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQU75806.1"
                     /translation="MLEEVRYISRDHYPYGMMMPDMDWSSENPYRLGFQGQERDDELK
                     GEGNSWTFRYRMHDPRIGRFFSVDPLTRKYPYYSPYQFSGNKLIHKIEYEGLEEQDPE
                     MLKIGYGSEGLSGFEYGTVSQTHVGGGLGISLSRYTENSFGLNYHFSSSLDTYVRKNN
                     VNVIGASSITKASFGGTLFRGFTIMWVVESHFDVGELHSVSTITKGPRLFYELKNGTT
                     FQLGISNDFDWFNTMDFGYNGYTKSFGPAQDGGITMNGELGVYWNEMNNALIFNAWGV
                     TPHRIPDSSQPQGYALSSETRDGRQIYQTENTKGSYFGIGKGTYYFYWPRLNSTIGVS
                     VGYEGQLLGKEIQDWIHFKPQFS"
BASE COUNT         3703 a         3231 c         3407 g         3945 t
ORIGIN      
        1 aaatcagaaa caaaatattc tgaatgccat caacaattcc gtcagcatca acaattttaa
       61 aaaagagtat gaatcaaggt gggcaggaca ggttaaaacg gcaaacctgt tattcccaac
      121 tattagaaac aatgtttccc agaagtctat aaagacattt gagtgggggg ataaatggcc
      181 ggaagatgca tggttgttcg ctgatatttc cgatggcgga ttcatccatt gcgaaaagat
      241 cgaagtgcag gaaggatgtg tgttgatagg agcacagttt ataaagtttg gaaaccggct
      301 tgctgttcag gcctcacagg cggagatcgc aacaaacctg gcagtacctg ttgaggcagg
      361 ctcaattgtc aatggcctcc catctgtggc aggtccccat aactgggaca taccaggtaa
      421 aaatgacccc gatggatttt ttgaagcccc aaaagggatt cgctggaata ccgactacat
      481 cgatttgccg gatgattatg tggcaacagg gatcaaaatt tattgtatca acaatgtggt
      541 tggaatcaag ctgaaggcga caaaactctt gggcgatgta aatacggaag atattaagct
      601 cagtacacga gcggctgata cggcatggtt tccggatgat gcaggtgcag gatcaccctc
      661 atccagtctg accatagaca acactccgcc aaaccacacc ggtgcctggg ctgatatgca
      721 tccggtggtg gctgatccac ctgcatatat tgtaggaggt ggtgtgtatc aatatggcaa
      781 ccgggtagct attcttctga cacctgcgtc gattgaaata ctgaatccat aacagcaaaa
      841 gacatcacta cagcaacggc atgtgatgcc atttatttag atatgataat ttcaatatat
      901 cagataaaca ggttaatcct gttgctcagg tttgaaatac cgtaacctgt ataccggtct
      961 gatttcttct atcttttcaa tgacctgtcg gtataccagt ttacctggtt gaccgcagca
     1021 gccagcgcag gtactggtct ttactactac ccacccgggg ggaatgtcct gatcggggca
     1081 tatctccacc ctggtgccta acggatacag gtcgatgcgg gtgatctcgg gctgatagac
     1141 aagttgccct ggctgtgcgc aacagccggc acaactcgtc atatcagtaa tgacccaacc
     1201 ccagggaagc gtttgcgggc aaacagtcaa ttctgtaccg gcaggcaggt gatcggtttt
     1261 tttgatgacc ggctgataca ccagttgccc cggctgaccg cagcaaccgg cacaggtttt
     1321 atagtctgtg atgacccaac catccggcat cggctgcgga caaacggtca gttccgtttc
     1381 cggtgccagc gtatccactt ttcggatgac aggtctgtaa tcaagttgtc cgggggtaca
     1441 gcaacctgcg caggtttcca cgtctatcac cacccatccc ggagggatcg gctgatcggg
     1501 acaaatgatc acttcctgtg aaaaaagctg gcttgtccat gcagctgaca acaggataag
     1561 gatactgaca ggaattgcct tcatatcgac cttggtttac aggatcattt tgtctgcagg
     1621 atctcatcga tcacccacct agcgccaccc aggtattctg tgatgaacat ggtataacga
     1681 atgtccacgc tgatgttcct gcaacggtcg aagtcataca tgatatcact catagtgcct
     1741 tcccacaccc ggtcaaagtt ggtgcctatc agttcgccat tggcattcag taccggactt
     1801 ccggagttac ctccggtagt gtggttgctg gccaggaaag ctacaggcag atcgccgttg
     1861 gcatccgcat agcgaccgaa gtcattgccc tgtagtaatg caagcaactg gtcaggcaca
     1921 tcgaattctt catcgccgtc cttatacttt tccaggatgc cttcatgtgt tgtataaggc
     1981 ttatagtaca caccatttcg gggagtataa ccattcactt gtccgtaggc cacccgcagg
     2041 gtcgagttgg catctggata gagcctgtct ttaagtccgc tggccatgat gccggccata
     2101 tacaactgca tttttctgtc gatggcctcc tgcagaggtt gaacagagtg attgtttgtc
     2161 aacaggtact gctgcaggaa agcatcatat aacatgtaca caggatcgtt ggccaatgca
     2221 tctttttcac ctttagcgat ccggtctaaa agattatttg ttccctccag acttaccagg
     2281 gcactgctgt tccacaaaga ttcagcccag gtaacaaaat ctcctttata cctgactatc
     2341 tgttccagga aatagtctgg cagcaatgca gtatccacct gttcatacac cctcttcatc
     2401 aacagtggaa aaacagcctc atcgatagga tggtaataat tggagaagtg cgatgctgca
     2461 gtattccgca tgtcgtcaat agcctgagct atgcgatcct tatcaggttc ttcttcattc
     2521 agtctcgtgg caagtccgcg aaaacttctc gcaaaacgaa tagcttccac accgagcgct
     2581 ccttcaatgg tatgatccag aaaaccggtg taagcattga gggcacgata caaactgtcc
     2641 aattcatcag ttacacctgc atacggtttt ccggcaggcg tggagcacca gtcgtcgaag
     2701 cggtattcaa attccagctt tttatcaata gcgttattgg cagtgaggcc gagtgtttcg
     2761 ccctgccatt tcttgtaggc attggccagg cttttgacct tactggcata catgagggtg
     2821 atggtatcgt tgccgtgcat aaaggattca gcggtacgga tacgcagttc gcgaagtgct
     2881 accctgttgg gattctgttc gttcataatg agttctacag ctgcagcggg caggtactcc
     2941 tgggtgcggc cgggaaagcc gaagaccatg gtgaaatcgc cttctttggc acctgccagg
     3001 ctgacaggaa agtggtacct cggaacgaag ggtttgttat cgggcgaata ggctgctggt
     3061 ttattatcag gaccggcata gatgcgccag atactgaaat cgcccgtatg tctgggccat
     3121 atccagttgt cagtttcccc accgaactct ccaactgaac gagggggaac gcccaccaac
     3181 cggatgtctg tgaactcctc ggtaacaaat aagaagtact gattaccata gtagaacgga
     3241 cgaacatatc cgccataatg ggagtctttg gtggcagcca cgagaatgga atctgaacgt
     3301 tgctggatga tcacagcgtt gttggcggat ggatccagtc ctttcagaat actgctggtc
     3361 acatcttcca tcctgacgat gaaggtgacc ttcaggccgg ggccgggaag ttctccggcc
     3421 atgttttcgg cccagtaacc gtcccgcaga taattatgat ctacggagct cagctgattg
     3481 acggcatcaa atccgcaatg gtgattggtg agcaccagac ctttatcaga aatgacctct
     3541 ccggtgcaac ctcctccaaa gatcagaact gcatccttca tacttccctc attgatcgaa
     3601 tacacatctt cggcatccag ttgaaatccc atggcatgca tctcgtcttc attggtttcg
     3661 agcagagagg gaatccacat accttcctgt gctgatgcag aggaagtaag ggagccgata
     3721 attataagag taaagagctt attcattttc atagcagata aaaataagga actacacatt
     3781 gatgttcgga attaccgacc gatgatttca tccacatttt cccggatggt ctcatagatg
     3841 gcatcggtag gcaggtcatt ttcatcttta ccgaaggggt cttcaatttc ttctgcgatc
     3901 atttctatgc tgataaaaat atagaagagc agaggaacta taagggcggt ccagtagctg
     3961 aattgttggg caaatcctat gggcatggtg aaaatgtaca ggaagatgac cttcttcaga
     4021 aagatgttgt aagagtaagg aatgggcgtt tttttaatac gttcacaggc gccgatgttg
     4081 tctgtgaatg actgcagttc attattcaga tacagaagtc gctccggttc agggttctca
     4141 ctttgtttcc atacctcgat ctctgaatac atggccttca tgacctggct tggcacgtgg
     4201 ccctgtctgt tgtaatatgt tacggggtat ttgtcgcatg gttccagctc gcgaatatct
     4261 accggccccc ggaggtgctc ctttgcagcg tagatgtagt tcggaatgag aatacgaaag
     4321 cgctcccgca actctttact ttgcataagc acggaaacct tgagtgcgag gttgcgactg
     4381 ttgttgacaa agctgcccca gatctttcgg ccttcccacc agcggtcgta ggcagaattg
     4441 gtgcgaaata caagcagcag agacaaaaca aaccctacca gagaatgtat gactgttgta
     4501 ttgacgaatt ccagatgtaa gatctccttt tccacccaaa cggtggccgt ggcatatgca
     4561 gcaacaccca gtatgccggg caaacttctt ctgaatgtgt cactctttct gaatgcaaag
     4621 atcagtttcc accagtcttt cgggttgtag agtaccatgg cgataaagat acaggtgaaa
     4681 gagataaaga atgcaggagg gtgcagtttc tctcatcagg taacggtctg tcttgcagca
     4741 acactgcatg gtgatgaata gctttatgtt tcttatcagc ggaaagtaaa atcagttcag
     4801 acttgtgtga gatataagtt cagatctcac tgggaagtac gccaaattct gccttgaagc
     4861 ttttagtaaa ataagaaagg cttccatagc ctactttaat ggctacctca ctgacattgc
     4921 catcgccttt ttccaatagc tcttttgctc tggttagtct gaagcttcgg atgatctcag
     4981 acggactttt gtccaccagt gccttgagtt ttctgtggag ctggcttctg ctgaatgcca
     5041 ccgcgtcagc aagatcttct acagaaaaga actcattgtc catttgtttt tcaatgatat
     5101 cacttacttt ttgcaggaat ctctcatctg ctgaggtaac tgccagttct ttcggtttcc
     5161 agacatggtt gctgctgaaa cgttcccgta acttttttct ttgtgctacc aggttgcctg
     5221 ccctcacacg caattcatcc tggtcaaaag gttttgtaag gtagtcatca gcacctgttt
     5281 ccagaccggt aagcctgttt tcttttcctg cttttgctgt gagcagtatg accggaatgt
     5341 ggcttgtatg ttcatcttct ttcaattttt tgcaaagggt cataccatcc atctcaggca
     5401 tcataacatc acttatgatt atatcagggg tatattccag ggcagcagtt aaacccagtg
     5461 caccattttc agccttgatg atgcgataat gttcttcaag accttctgca atgaaggacc
     5521 ggaggtcagt attatcttct actatcagca aaagggggag cgggttatct gttatttcag
     5581 ctatgctatc aacaggttct tctgcatcag gcaaaaaatg cagcgtatgc cggttgaccg
     5641 gagtcttcgc agctgcaacg atctccgtgt tatggaagtt ttcctgatca accggcaggc
     5701 ttactttgaa ggtggtgcct tgcctgctgc tttctacggt gatctgtccg tggtgtaatt
     5761 caaccagttc tttcaccagg ctgaggccaa tgccggttcc ggtgtcttca gttccttcaa
     5821 cctggtagaa ccgttcaaag atcctatcca cttcatcctg gctgatgcct ggtccgctat
     5881 cagatacacg tatcttcagg cgtccttttt cgatggttga atctacagat acccgtcctc
     5941 tttcaggtgt gaatttgaat gcattggaca ataaattgac aatgatcttt tccagtttat
     6001 ctgcatcaaa aaataccttc atatcagcct gtggatagga ggtctggaaa tgtatctgct
     6061 tgatctctgc caggctatcg aacgagccga cgatagcacg tagaaatgca gacagatctg
     6121 cccgggccac ttgcagctgc atgcttccgt tttccagttt tgacagatcc agcagctggt
     6181 caatcaattg attaagtcgt tcagcatttc tgcgcatcat ttggaggtgt ctggaagaaa
     6241 ccgggatatc gacatctgtt gtggcggaat cattttcgct gaccggtgcc tgttcctgag
     6301 cttttcttaa aggaccaaga atgagtgcaa gcggcgttct gaattcatgg gagatgttgg
     6361 caaagaacct gcttttgact gcatccagtt ccaggatccg ctctgcatct gaccttgtcc
     6421 tttcggtcag ttgcagctta taagcaaggc cgtaagaata gatgatcatc agccccattt
     6481 gtccaacagg ccagggattg aagagctgaa agtattctgc gctgtagagg atcccaaggc
     6541 agttgaaaaa aaggagccac acggcaccca cagcaaagat cttgggcagc cttcctggca
     6601 gaaatgccat gcggatgcat aatgccaata tagcaacgag caatataccg gaagccagag
     6661 tcaggtctgt tcctgaatga cgaacggcca gggttgtatc aagcaagaat tgcagtacga
     6721 tcaaccagga tgcccagcgc acatatttat caattcgggg agctacctta ggcaggttaa
     6781 caaaatgcct tccaaattgg agaaagaata gttcaccgaa ggtggtgaag aagatccata
     6841 catattctgc cagccagggc ctgtttggaa tgaaccagcg tacaaaccac agatcgttgt
     6901 gaatgatgct gtagtgcagc atcagtgtaa cgcacagcag gctgaagaac agattggctt
     6961 tatcacggtc caccagaaaa aacatgaata ccagtatggc cagaatgcct gttacgccca
     7021 tactgattcc catgagcaca gataaccctt ccatttccgc aggcaacgcg atggcatccg
     7081 ggcgcacctc tgctgataaa cctgtagtaa agtctgactg attgaacagt cttacataaa
     7141 cagtcagggt atcacctgca gaaagtgtga aaggcatcct gttcatcaaa ggatggctat
     7201 acaagggtct gtcttttgca ggcacaagaa atccatcact aacccttgtc atcttgccgg
     7261 atccatccag gaagtatgca tctgcgaact cgtttcctcc cgtaaaaaca gaacgattca
     7321 cagaattctg atagtcggga ttcttaatga tcactgcctg gtggttgata tcttcggcag
     7381 atgttaaccg gaacgtaagc cagtacgcaa ccggcgtatc gctttttcca actgagtcgg
     7441 tcaacggttg aaatccggta ggagtatgcc ccgccagaat gtcttcaatg gtcagtgtac
     7501 aacctgaatc taccagtacc agcatcttct catcaagatt gtggatctcc aggttatcat
     7561 gtatctccac tatctgttgc tgtgaaaaca gataaacaga ccaaaatagc aatatggaca
     7621 ggaccggaat tctcattttg tgttttgaaa ataaaaaaaa tatgaacagt gatgcaagga
     7681 catcactgtt caattaatag caacttattg ataccactgt ctgaactggt ttcgggcaaa
     7741 cccctctata gcttcagctg atggttcgtt cgttttcagg atctcgttgt actcgatgtc
     7801 ctggtttcca aaagagatga cctcccagcc gttcgcagtt ctttgcacaa ccatggagcc
     7861 taccatttca ggccagtttt tttcctttgt tccggtctca taggtattgg ccagatagtt
     7921 ggctatagca atatcatcgc tcagaaacct gatcctggtc atatgcatgt gaagattggt
     7981 gtgttgaaac tgggtgtcaa acagaccctg gtgggtattt gcgttggttt ctctgtccat
     8041 gtccggcaga aacataccga accagatggt ccagtcgtgc tgttctgcca tatggctggc
     8101 ccagagttgc ccgttcttcg tgttccaggc tttttccatg tcagacagga tttggttgat
     8161 gttcttttca tcctgcgatc tgttttgtgc aatgctcaca tggtggaaga atagcacagc
     8221 gatgccaagc atcatgatac gaatggtctt catttgaatg gatttattgg tgataaaaaa
     8281 gttttcaagc tgtttgaagc tggccgacat tttctgtgcc ggctttcaga tctgtgtata
     8341 taagcaggtg tgcaatcagc aggatcagat tgtcaatatg tgccagcata tcaatacgtg
     8401 gaatacggct caggggaaag cagagggcac caatgaaaaa caacacaccg gttagcaggc
     8461 ggatcttttt cttcctggtc agctgcacac tcaggatcat catgctcaga gggaacaggc
     8521 ctccgggaat aaacagaaaa gccagtgtcg agctacctat acttccggta aattcagcag
     8581 ctgcttccag atcggtgaaa cccatggcct cagtatatat accatccact ccgaagttat
     8641 ttcctccaat acaggcatat gctgccagaa taaatcctat tactgaatag gttggcagat
     8701 cttttctgat gacctgaaat aaaccctgga atgaaaatat ccatacggtg aatgccagta
     8761 cctgcaagac acctgcagtt gtggtaaccc ggccatcatc ccagaagaac tgggctgcag
     8821 ccagcaggat cggggcgagc acgagactgg tattccagac ttgtttctga agtgtttgtt
     8881 tttccatttt ttttgctttt aagtgtgaat tatttggagt agtaattgct gatctctgtg
     8941 aagaaggcgc tgtaatgctt ttgaaaagtg cctgatagat cctggtagat cgttctgctc
     9001 atgataccga cggtataacc ggcaaccagc agcagagcta tcggtaacat ccgggacaaa
     9061 cgatggataa tggatactgg tttgtgcgac atcttgtgtt cgttttgggt gttaagaatg
     9121 atgacgcaaa gatgaaatca cctgccaggc tggtgttagc aggttggtcg caagaagtat
     9181 aacattcgtc gcacaagccg tcagatgcta ccagattgcc agttttgcga cgtgaacaac
     9241 ctcaggtcag ttggtagaac tgagcagatc acagaaaatc atgtctccgt tctgggtact
     9301 cagccagata tgtgcaatgc tgaccatgac ggggcattca tatcactatt ctggagcatg
     9361 tttttcagga atagcagact tattttcccg tcatccccca aaaaaaaagg ccgcctgacg
     9421 gcggccttat aacccaaaac tgcaaaatca aacgtccaca cccttcagca tcttgttcca
     9481 atacagatag ggcaggccgt actttttcag catccagaga cgccagtgtt ctttatcgct
     9541 gttgaatacc agcatttgct tcagtttggg gtcaggtgtg aagttgttct ggtaatcgaa
     9601 ttctgccagc accatcttgc cataaccggt taccagcggg caggaagaat atccgttgta
     9661 ttccttgctt cccagtttgt tctcctgcat caggtgtacc aggttgttga ccactaccgg
     9721 cacctgcttg cggatggcag ctccggtttt ggcagtgggc agagcagcca catcaccgag
     9781 cccgaacaca ttaggatacc tgttgtgctg caaggtgttg atgttcacgt ccagccatcc
     9841 ctgtgcattg gcaaggcgtg attcacgtac gaatctcgga gcagcctgcg gaggtgccag
     9901 gtgcagcatg tcatagggga tggaaatatc ggtatcacct accatggtct cgcccaggct
     9961 gttgtcttca ttgatcacgc aacggttctc ttccggagca ctatgcttga aatggatgat
    10021 cttgttctcg ctgtcaatgc gatgaggtgc atagaagggc ttgaaatgaa tgtcgtagcg
    10081 gttgatgacc tccatcaggg tatgggcaat aggcttcaca ccaaaaatga ccgttcccgg
    10141 agtggcaaag atgacattgg acttatctgc aattcctttc ttacggaaat aatctgctgc
    10201 cagataggcg atcttctgcg gtgcaccgcc gcacttgata ggtgtggtgg gctgcgtaaa
    10261 gaccgcattg ccgcctttga aattcctgat cacctcccag gtgtattccg ggttggtgta
    10321 attgctgacc acagtgcctt tgtccagggc ttctgccagt ccttctatca tggtaggttc
    10381 catgaccaga ccagtggcaa ctaccaggta atcataaggt atatcaccgt tcttttcggt
    10441 gtgcaccaga ttgttctccg gatcaaaacc ggttgccttg tcctttaccc agttgacgcc
    10501 ttttggcatg acactgctca tggagcgtgc ggtcttcttg aagtcgaaag tgccggctcc
    10561 caccagggtc caggctggct ggtagtaatg ggtatcaccc ggttccagca gggtaacatc
    10621 cacatttttg tgttcctttc gaaactttgc agcgaccata atgccggctg taccaccgcc
    10681 aatgatcaga agttttgttg aagccattga tttgattttg tggttagggt tttcaggata
    10741 tacgaattcc gtatgcctga atggaagtca aatctgggga aatacgtgat ggttgtatgt
    10801 aactgatatc acattgagtg gaagggcatg aaatcagatc tgaaattgtc tttcagttct
    10861 ccttcagttc ctggtccagc agatcgatgt tctcttcaga aacctacagt atctggcgga
    10921 agagatcaga atattccaaa gaggtaagcg aagcaatggc acagccgttc ctgtacacag
    10981 ggtcgtggag taagccctca aagttggcga cggtcaggcc aagttcgccg gcggtatgct
    11041 ccttcagaaa tatctgtgcc atcttatcta catctgcatg tgtgaagatg ggtttggaga
    11101 ggtactgctg gtactcgtag gtctcgtgct ccaggttgga agtattggac tgatacaaca
    11161 attccaggtc gaagagtttt tccttgatgg aggcattcct gatcagattc atgttacccg
    11221 aaccgagcag atcttttacg gtcacattac tgggattgaa ccagtattca tacaatgaca
    11281 tgatcacatc cagggccagg ctgtcgggat cctgtggttg tatgccatca caataggtaa
    11341 tgagtctgtc caggctttga atgcgatctg cccggaagtc gatgaggaat gtcagattct
    11401 ccacgtcctt gtccaggtca agacgaatgt tctgcaggta tttggtctcc cgctcgtgct
    11461 ctacccgtgc gatgttgttg ttgttcactc tcagagccaa taatataccg atcatgacca
    11521 gaacgatctc gccaatggca tagagcaggt attttcgaaa tgcaccacca ccgatgagcc
    11581 tgcttcggag atttctgaat atgcggatca tgagaggaag ttagttattt gttccatttc
    11641 aggattcccg caggatcttc tccatcttct tccccttggc cagctcatcc accagtttat
    11701 ccatataccg gatcttttgc atgagcgggt cttcaatatc ttccacccgg tagccgcaga
    11761 taacaccggt aatgagtgag gcattgggat ggatctgtgg tgcttgtcca aagaactgtt
    11821 ccagacttgt tttctgatcg atctgttcct gcagaccttt ttggtcatag cctgtcagcc
    11881 agcagatgac ggcatctacc tcttctttgg tacggccctt cttttctgcc ttggtgatgt
    11941 agtgcgggta cacaccagca aaggacatct ggaagacacg gttgtttgac atactggtct
    12001 attcgtttgg ttcgggttgg atgctgatga ggctcaaata tagttgttgt atccgttcct
    12061 gttcagtgac cagatgatca tcagcaaaag gggctccgca atcgaagtta attgcctgat
    12121 gcaatagttt gccgtttctg aagtaagacc tgacatctga tattccttct tcaggcacga
    12181 gtgttctttc gcggcacagg atcatgtcat tttcccggaa gaagaattcg gtcattcgga
    12241 ctgcagggta tgaaccccat ttcatgatga tccattgcag atcccagctg ttatacaaat
    12301 agtagatcgt tgcattcagg ctgtctatct gagccgttgc actgatgacc ggcatcaatt
    12361 cctggcaagt cagataatat acatagtctc tagcaggacg taattcttct ctcaaataat
    12421 catttaccgg ctcatcgtag tagcacacag agggcaggag agtggtgtct gcaggttgtt
    12481 gtgcctgcac tatactgtat gaaagaacta aaatgatgac cgctttgctc atcgattcag
    12541 atcctgttat ttggtcttcc ctttgaacgt ggcatagatg gccatggcat ggccgtgccc
    12601 cagcccgaac tcttccttca gccagttggt gatctgtgtg gctttaacat ccgggttgag
    12661 cttcccgtca atgagaaatt ccttttgctc tgccagcttt ttgaaatctg ccggcgactt
    12721 acctgttttg gctttgatgt tgtctatgta ggcctggaat gacatgggat cttattttaa
    12781 gtttcagaca ttttactggc aattggatgt ggcgttgaag atacaaatct agatctgcgg
    12841 ttaatagggt cactacccaa agtaaaatga ttgcaatgtt tagatttcaa gattggaata
    12901 agcctgctga tagaaattac aatcgggttc atagaaatta ttaactgaat taactcagct
    12961 tttttgtgac ttggtttctt ctattagaat gatgagatca acgatagcaa aactactgtt
    13021 cataagaatt cctgagataa taaatggatg gaattctgcg cacttcaaaa ccattgccga
    13081 gcgtgagaaa aatgcggaat tctcaaaata aactagacaa agggaccgat aatttatgca
    13141 gcaaattgga gactatttga aattgtaaat tagcattatc ttgatgaaat ttcaacatta
    13201 gtaggtactg taagtgcttg aggaagtgcg ctatatcagc agagatcatt atccgtatgg
    13261 gatgatgatg ccggacatgg attggagctc tgaaaatccc tataggcttg gttttcaagg
    13321 ccaagaaagg gatgatgagt tgaaaggaga aggaaatagt tggacattta gatatcgaat
    13381 gcatgaccct cgaataggtc gattcttttc tgtagatcct ttaacacgaa agtacccata
    13441 ttattccccg tatcagttta gcggaaataa attgattcat aaaattgaat atgaaggcct
    13501 cgaagaacag gaccctgaga tgcttaaaat aggttatgga agtgaaggtc tatctggctt
    13561 tgaatacggt acagtttcgc aaacacatgt tgggggtggc cttgggatat cacttagtcg
    13621 ctatactgag aatagttttg ggctaaacta tcacttctct tccagtcttg atacctatgt
    13681 cagaaagaac aatgtaaatg taattggagc aagctcaatc acaaaagcat catttggagg
    13741 aactttattt agaggcttta cgattatgtg ggttgtagaa tcacattttg atgttggtga
    13801 acttcatagt gttagtacaa taacgaaagg tccaaggctt ttttatgaat taaaaaacgg
    13861 aacaacgttc caacttggca ttagcaatga tttcgactgg ttcaatacta tggattttgg
    13921 atacaatggc tatacaaaaa gttttggacc cgctcaggat ggcgggataa caatgaacgg
    13981 cgagctagga gtctattgga atgaaatgaa caatgcgctc atttttaatg catggggagt
    14041 gactccacat agaattcctg attcaagtca gcctcaggga tatgcattaa gctctgagac
    14101 tagagacggg aggcagattt atcaaacaga aaacactaaa ggaagttatt ttggaatagg
    14161 aaaaggaaca tattactttt attggcccag gttaaactct actattggag tttcagtggg
    14221 ttatgaaggg cagcttcttg gtaaagaaat tcaagattgg atacacttca aacctcaatt
    14281 ttcaca
//
LOCUS       DAOMAX010000046        14192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_501_length_14192_cov_4.155266, whole genome shotgun sequence.
ACCESSION   DAOMAX010000046 DAOMAX010000000
VERSION     DAOMAX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_501_length_14192_cov_4.155266"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK742_04120"
     CDS             complement(<1..310)
                     /locus_tag="PK742_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQU75807.1"
                     /translation="MRHTLLLLLLGAMVCTPMQAQIVINEASNRNASQTADEDGDFGD
                     WLELYNAGVGSVNLEDFGLSDDPSDPLMWTLPDITLDPGAFLLIYASGKDRKPDGGID
                     H"
     gene            424..1452
                     /locus_tag="PK742_04125"
     CDS             424..1452
                     /locus_tag="PK742_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQU75808.1"
                     /translation="MAYLHNRISHRELKERLMAENFHRTTISFYRYFPVHDPQTFRDT
                     LFANLDAMQVFGRIYVAEEGINAQISVPSHRLEELRSYLNTINGLEDLRLNVAVDDDG
                     KSFWLLKIKVREKIVADGIEDPTFSMENKGKYVRADAFNHLATDPDTIIVDMRNHYEY
                     EVGHFRRAIEVPSDTFREQLPMAVDMLQEYKDRNIIMYCTGGIRCEKASAYMLHHGFQ
                     HVYHLEGGIINYAHQVQEEQLDNVFIGKNFVFDDRLGERISDDVIAVCHQCGNPCDTH
                     TNCINSACHLLFIQCPACAEKYGGCCSEACFTFIQLPEEEQKEKRKGIHKEHNIFNKS
                     RRRLRPMN"
     gene            1454..2854
                     /locus_tag="PK742_04130"
     CDS             1454..2854
                     /locus_tag="PK742_04130"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQU75809.1"
                     /translation="MKKLISVPVLLLSLAAVAQSTIDESILEHMDNKHVSGLAAVIIS
                     NDGIEWEGYYGLANRQADIPVTDSTIFMLASISKTVTATALMQLWEDGLFELDDPVND
                     YLTFDVMHPDHPETEITIRQLLTHTSSIKDNWDVMDPLYVNGDSPIALEDFMNAYFTP
                     GGEYYSATANFYAAAPGSGYHYSNIGAALCGSLVEAITGTAFNTFCNEHIFDLLCMEH
                     TAWFLSELDESLIAHPYSYFAGFYTDEGLYGYPDYPDGQLRTTARSLAKFLFTNMTGG
                     SFEGNTLLESATIDSMRTIQDPLIDDEQGLIWYTWSYNGATMWGHNGGDAGVVTDMFF
                     DPATQRGYIILTNGNAFNGPVMSILMNSGPDDWYEEPIAMSCELPVALSGSSDEVQLS
                     ISPNPAANHTRIMGTPVPGSRYRISDMEGKVVCNWTLVPANGFISTTELLNGSYLLEW
                     SDGRSATACVLMVQNR"
     gene            2908..4209
                     /gene="metK"
                     /locus_tag="PK742_04135"
     CDS             2908..4209
                     /gene="metK"
                     /locus_tag="PK742_04135"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQU75810.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALVDNFLAWDPQSKVACETL
                     VTTGQVVLAGEVKSKTYLDVQEIARQVIRDIGYTKSEYMFEANSCGIVSAIHEQSPNI
                     NQGVDKEDRKLQGAGDQGMMFGYACNETQDRMPLALDLSHRLLWELSQIRREGKVMTY
                     LRPDAKSQVTIEYSDDHKAMRVHTIVISTQHDDFIKPANQSEAARKAAEKKMLAQIYA
                     DVEKYLIPRTMKGLSASVKKLFNSKYILHVNPTGVFVIGGPHGDTGLTGRKIIVDTYG
                     GKGAHGGGAFSGKDPSKVDRSAAYAARHIARNMVEAGVCDEVLVQVAYAIGVAQPVSL
                     HVNTYGTAKVDMNDSAISEVVGGLFDMTPYAIEQRFKLRTPIYSETAAYGHMGRSSKK
                     VKKTFRKVENGVEKVKTMEVVLFPWERSDYVPKIRKAFGIR"
     gene            complement(4210..5073)
                     /locus_tag="PK742_04140"
     CDS             complement(4210..5073)
                     /locus_tag="PK742_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HQU75811.1"
                     /translation="MRQLKITASITSRDSDSLEKYLSEISRIDLISPEEEAALARQIR
                     EGSDEALEKLTKANLRFVVSVAKQYQGQGLSLSDLINEGNVGLIKAAKRFDETKGFKF
                     ISYAVWWIRQSIMLAIVEQARLVRMPLNKVGSYSRLNRLSQQLEQEYEREPTLEELAE
                     IMHMKEDEVKDITRGHHRHVSVDAPLGDSEEGSMLDTMSDTDDRSSPDKDLVSDSLKK
                     EIEEVLNVLNYREREIIAAYFGINGTPQMNLEEIAESLDLTKERVRQIKERAIRRLRK
                     TTTSTELKSYL"
     gene            complement(5160..7292)
                     /gene="pnp"
                     /locus_tag="PK742_04145"
     CDS             complement(5160..7292)
                     /gene="pnp"
                     /locus_tag="PK742_04145"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQU75812.1"
                     /translation="MNPIQKTFDFGDGRTMMLETGKLAKQADGSVVIRMGDTMLLATV
                     VSAKEAKEGIDFMPLTVEYREMYAAAGRFPGGFLKREARPSEYEILISRLIDRALRPL
                     FPEDYHADTQVIVNLISLDKEILPDALAGFAASAALACSDIPFGGPVSEVRVGRINGE
                     YVINPTRSAMEQSDMDMIIAGTVDNIMMVEGEMKEVSEKDMLGAIKFGHETIRRQCEL
                     LLSLQSELGKDTKRTYSHEVHDEALRDTIMSNLREKVKAVAYGGTAKQERSDAFKAII
                     TEYIETMSEEERAEKEGLAKRYYHDLEWEVMREMILADHKRLDGRALDQIRPIWTEVD
                     YLPSVHGSAVFTRGETQSLTSVTLGTKLDSQLIDGVVFEGESKFLLHYNFPPYSTGEA
                     RPLRGTGRREIGHGNLALRALKNMLPEENPYTIRVVSEILESNGSSSMATVCAGSMAL
                     MDAGVQMKKPVAGIAMGLISDPGMTRYAVLSDILGDEDHLGDMDFKVTGTADGITATQ
                     MDIKVDGLNYEVLEKALDQARNGRLHILGEMAKTITAPREDYKPMVPRIEKMTIPREF
                     IGAVIGPGGKIIQEMQRETNTTINIEEVGEFGIIDIASPDKASIEAAKARIRGITAMP
                     EVGEEYEGTVKKIVDFGAFVEFLPGKEGLLHISEISWTRLPSMEGVLKEGEKVKVKLL
                     EVDKKTGKFRLSRKVLMERPQAPAKNEE"
     gene            complement(7371..7640)
                     /gene="rpsO"
                     /locus_tag="PK742_04150"
     CDS             complement(7371..7640)
                     /gene="rpsO"
                     /locus_tag="PK742_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQU75813.1"
                     /translation="MPVTAERKKEIVKEFGGSEKNTGSVESQIAIFTERINMLTSHLQ
                     ANKKDHATKRSLLMLVGKRRRFLNYLMKKDIEGYRALIARLNIRR"
     gene            complement(7721..8575)
                     /gene="accD"
                     /locus_tag="PK742_04155"
     CDS             complement(7721..8575)
                     /gene="accD"
                     /locus_tag="PK742_04155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQU75814.1"
                     /translation="MSWFKRMREGIQTSTREKKDTPEGVWFKCPDCKKTTTTTDYEDH
                     LWCCPNCGYHGRVGSHEYFQILFDEEPKLLFEEIISTDALGFVDLKPYSKRLDEARKK
                     SGYSDAISVGHGTIEGQGVVIACMNFPFIGGSMGTVVGERISRGIDYSISHKLPFIII
                     SKSGGARMMESGFSLMQMAKTSAKLTQLARHGLPYISFMTDPTTGGVTASYAMLGDIN
                     MAEPKALIGFAGPRVVKETIKKDLPEGFQQAEFVLETGFLDLIVERKDLRSEMARLLA
                     YMMPDTGK"
     gene            complement(8651..9724)
                     /locus_tag="PK742_04160"
     CDS             complement(8651..9724)
                     /locus_tag="PK742_04160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HQU75815.1"
                     /translation="MSISNISSLLGEKAEYLLNHECKTISKKDLHLPGNDFVDRVWMQ
                     SDRNPQVLRSLQTLFGNGRLANSGYLSILPVDQGIEHSAGASFAANPIYFDPENIIKL
                     GIEGGCNAVATTFGALGTVSRKYAHKIPFIVKINHNEFLSYPNKFDQIMFASIKASWD
                     LGAVAVGATIYFGSDESTRQLQEVARAFEYAHELGMATILWCYLRNPGFKKDGVDYHV
                     SADLTGQANHLGVTIEADIIKQKLPENNGGYLALNTKESAYGKNDKRIYTELTTDHPI
                     DLCRYQVANCYMGRAGLINSGGASSGASDLAEAVATAVVNKRAGGMGLISGRKAFQRP
                     MKEGVGILNAIQDVYLSNEVTIA"
     gene            complement(9832..12027)
                     /locus_tag="PK742_04165"
     CDS             complement(9832..12027)
                     /locus_tag="PK742_04165"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQU75816.1"
                     /translation="MIFRKINMLVLTAVIGVSSVSASGPFPAPDEGMWLVSMITNMNI
                     QEMKKIGFELEAEDIYSLDKPSLKDAVMVFGGFCTGEFVSANGLVFTNHHCGYDAVAG
                     ASTTEHNYLDNGFWAGSYDEEIPIEGLYIELLVRADNITDSILPLVKDLDESARGMKV
                     REIIDRITAREEVSSGLGVEIKSMYNGNQYYIFYRKRYNDVRLVGVPPQSIGKYGGDT
                     DNWEWPRHTGDFSIFRVYADASNEPAEYSENNKPYHPKRHLKIDISGIQEGDPAMIMG
                     FPGSTNRYLTSWAMKEVIYETNPAQIDMFKAVTDAMKVEMDKSDAVRLELSPDYASYM
                     NVLKLYTGQIEGMTRVLDAVAVKEAEEKAFNNWLARQDKNTQEMYGGLMDEYASVYGA
                     LSAINREFYYKRYAVILPSTAGIALTLQGLEPLLADKKASKEDKDAVIAEIQAAGQEA
                     WPTMYGNIERDVVTSMLIVMNNNLPEAQKPESLKNILATAQGMNDDEKITNWAKNAFA
                     TSIVTSESRLNAFLAKPKLKTLQNDQVYRLFTNINEEGLAKLADFRGNRRELSRLNRE
                     YTAATMKMHPDKAFYPDANFSMRLTYGKVWDYSPKDAVNYHWQTYLDGVIEKMDNNDE
                     EFMVPDALFNLYKAGDFGRYADETGDVPVCFLSDNDITGGNSGSPIMNRRGDMIGLAF
                     DGNYEGTPGDYVFDPKMNRTINVDIRYVLFIVDKMANCQHIMGELDFAE"
     gene            complement(12105..12458)
                     /locus_tag="PK742_04170"
     CDS             complement(12105..12458)
                     /locus_tag="PK742_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75817.1"
                     /translation="MKNLYALLLSVFLLGSLQAFSAVRPEGEPDVASHVDGQTSLAIF
                     PNPVKTEATISFSADVERISVMNIVGREVLTVEVQSGTNFLKIDLSELQPGVYFLSAQ
                     SQGKNLLTKRFMKEL"
     gene            complement(12552..14078)
                     /locus_tag="PK742_04175"
     CDS             complement(12552..14078)
                     /locus_tag="PK742_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HQU75818.1"
                     /translation="MSRPNTLGALKKSGYRPRSIKEELRSNLITKLRSGEPLFEGIYG
                     FEHTVIPDLERAILSRHNMLLLGLRGQAKTRIARRMVELLDEYIPVVAGSELNDDPMD
                     PISRYAKDLIAEKGDDTPIEWLHRNDRFTEKLATPDVSVADLIGDVDPIRAATLKVPY
                     SDERVIHFGLIPRSHRCIFVINELPDLQARIQVALFNMLQEGDIQIRGFRVRMPLDIT
                     FVFTANPEDYTNRGAIITPLKDRIESQILTHYPKDLETSRRITRQEARTHPDQEALVK
                     LGGIAEELVEAIAMEARKSEYVDQKSGVSARMTITALENLVSAAERRALRHGESLTWV
                     RMSDFWGVIPSITGKVELVYEGEQEGPFIVALNLIGKAVRNRFPSYFPAPESGRKPLG
                     KGREEGADIPADYASVVDWFNSGHQLDLLYDLPETEYRKALDKVTGLSDLVSNYYPQV
                     SPDEHYFLMEFVLHGLAEYSRLNKQILQHATVFGDLLSAMFQSDDSEDWEEEDEDDDF
                     "
BASE COUNT         3451 a         3618 c         3419 g         3704 t
ORIGIN      
        1 aatgatcgat tccgccgtca ggttttctat ccttgccgct ggcatagata agcagaaatg
       61 cccccggatc cagggttatg tcgggtaagg tccacatcaa gggatcagac gggtcatcgc
      121 tcaaaccaaa gtcttcgaga ttgacactac ccacgccggc attatataat tcgagccagt
      181 cgccgaaatc tccatcttca tcggctgtct gtgatgcatt ccggttagac gcttcgttga
      241 tcacgatctg tgcctgcata ggtgtgcaga ccattgcccc cagcaggagc aggagaagtg
      301 tgtgtcgcat gagtatgccg tgttaagaca aaataaagat accgttaagc gaaggatttt
      361 gaaaactgct acctttatgt aagaatttta atcgcaaggc aagcgatcgc tgaattcaac
      421 agtatggcct atttacacaa ccggatctca caccgggagc tcaaggagcg cctgatggcg
      481 gaaaatttcc accgcaccac catttccttt taccgttact ttcctgttca cgacccgcaa
      541 acatttcgcg atacgctgtt cgctaacctg gatgctatgc aggtattcgg caggatctat
      601 gtagccgaag aaggcatcaa cgcccagatc agtgtaccat cccaccgcct ggaagaactg
      661 cgcagctacc tgaataccat caatggcctg gaggatctgc gcctcaacgt tgcggtagat
      721 gatgacggaa agtccttctg gctgctgaag atcaaggtca gagaaaagat cgtggcagat
      781 ggtattgaag accccacctt cagtatggag aacaagggca aatatgtgcg agcggatgct
      841 tttaaccacc ttgcaacaga ccctgatacc atcatagtag atatgcgcaa ccactatgaa
      901 tatgaagtgg gccatttccg ccgggcgata gaggtaccat ccgatacctt ccgtgaacaa
      961 ctacccatgg cggttgacat gctgcaggaa tacaaagaca ggaatatcat catgtactgc
     1021 acaggaggga tccggtgcga aaaagccagt gcctatatgc tgcaccatgg ctttcaacat
     1081 gtgtatcacc tggaaggagg tatcatcaac tatgcacatc aggtacagga agaacaactg
     1141 gataatgttt tcataggcaa gaactttgta tttgacgatc gcctgggcga acgcatcagt
     1201 gatgatgtga tagcggtttg tcaccagtgc ggcaacccct gcgataccca taccaactgc
     1261 atcaacagtg cctgtcatct gctcttcatc cagtgtcctg cctgcgcaga aaaatatggc
     1321 gggtgctgca gtgaagcctg ctttaccttc attcagcttc cggaagaaga acaaaaggaa
     1381 aaaagaaaag gaatccacaa ggaacataat atctttaata aatcaagacg tcgtttgcgc
     1441 ccaatgaatt gatatgaaaa aactgatatc tgttcctgtt ctgttacttt cactggcagc
     1501 cgttgcccag tcaaccatag acgaatccat tctggaacac atggataaca aacatgtgag
     1561 tggcctggca gcggtgatca tttcaaacga tggcattgaa tgggaagggt actacggact
     1621 ggccaacaga caggctgata ttcctgtgac cgacagtacc atcttcatgc tggcatccat
     1681 atccaaaaca gttacagcaa cagccttgat gcaattgtgg gaagacggtt tgtttgaact
     1741 ggatgatcct gtgaatgact acctgacctt tgatgtgatg catccggatc atccggaaac
     1801 agagatcacc atcaggcaac tgctcaccca cacatcgtcc atcaaagaca actgggatgt
     1861 catggatcca ctgtatgtta acggagattc acccattgct cttgaagatt tcatgaatgc
     1921 ctattttaca cctggcggcg agtattattc tgccactgcc aatttctatg ctgcagctcc
     1981 cggcagtgga taccactaca gcaatatcgg tgctgcactg tgtggctctt tggtagaagc
     2041 aataaccggc acagcattta acaccttttg caacgagcat atttttgacc tcctgtgtat
     2101 ggaacataca gcctggtttc tttctgagct ggatgaatct ctgatagctc acccttactc
     2161 ctactttgcc gggttttaca cagatgaagg gctctatggc taccctgatt atcctgacgg
     2221 gcaactgcgc accaccgcac gctcactggc caaattcctg ttcactaata tgaccggagg
     2281 aagctttgaa ggcaataccc tattggaatc agcaaccata gacagcatgc gcacgataca
     2341 agacccactc attgatgatg aacagggcct gatctggtac acctggagtt acaatggtgc
     2401 caccatgtgg gggcacaacg gtggtgatgc cggcgtggtt acagatatgt tcttcgatcc
     2461 tgccacacag cgtggataca tcattctgac caatggcaat gcattcaacg gtccggttat
     2521 gagtatattg atgaattcag gacccgacga ctggtatgaa gaacctatag ccatgtcctg
     2581 cgaacttccg gttgcgctga gcggatcatc agatgaagtg caactgagta tcagccccaa
     2641 tcctgctgca aaccatacca ggatcatggg cacccctgta ccaggttcca ggtacagaat
     2701 ttctgatatg gagggaaagg ttgtctgtaa ctggaccctc gtgccggcaa acggcttcat
     2761 cagcactacc gaattgctca acggaagcta cctgttggaa tggagcgacg ggcgttctgc
     2821 aacagcatgt gtattgatgg tgcagaaccg ttaaagatgc ctgtctgaat ccctatattt
     2881 gcacatcatt ttacaatctt ttcacgcatg ccttacctgt tcacatccga aagtgtgtct
     2941 gagggacatc ccgataaagt ggccgaccag atctcagacg ccttagttga taattttctc
     3001 gcctgggatc cccagtcaaa agtagcctgt gaaacgctgg tcaccaccgg acaagtggtg
     3061 ctggccggcg aggtcaaaag caagacctac ctcgacgtgc aggagatcgc ccgacaggtg
     3121 atccgcgata tcggatatac aaaaagtgaa tacatgttcg aggccaattc atgcggcatc
     3181 gtatcagcca ttcacgaaca aagcccgaat atcaaccagg gcgttgacaa ggaagaccgg
     3241 aaactccagg gcgccggcga tcagggcatg atgttcggct atgcctgcaa cgagacacag
     3301 gatcgcatgc ctctggcact ggacctctcg caccggttgt tgtgggagct cagtcagatc
     3361 agacgggaag gaaaggtcat gacctacctg agacccgacg ccaagagcca ggtgaccatt
     3421 gagtacagcg atgaccacaa ggccatgcgg gtacacacca tagtcatctc tacacaacac
     3481 gatgatttca tcaaacctgc aaaccagtca gaagctgcca gaaaagccgc agagaaaaag
     3541 atgctggctc agatctatgc tgatgtagag aaatacctca tacccagaac catgaaggga
     3601 ttgtctgctt ctgtaaagaa actgttcaac agcaaataca ttctgcacgt gaaccctacc
     3661 ggtgtatttg tgatcggtgg accacatggt gatacagggc ttacaggtag aaagatcatt
     3721 gtagatacct atggtggaaa aggcgctcat ggtggtggtg ctttcagtgg aaaagatccc
     3781 agcaaggtag atcgttccgc cgcatatgca gccagacata ttgcccggaa tatggtagaa
     3841 gcaggggttt gtgatgaggt actggtgcag gtagcatatg ccattggtgt tgcacagcct
     3901 gtcagcctgc acgtgaatac ttacggtacg gcgaaagttg atatgaacga cagtgccatc
     3961 agcgaagtgg taggtggtct gtttgatatg acaccatatg ccatcgaaca gcgcttcaaa
     4021 ctgcgtacac ctatttattc ggagacggca gcctacggac atatgggacg ttcctccaag
     4081 aaagtgaaga agaccttccg caaggtggag aatggcgtgg aaaaggtgaa gaccatggaa
     4141 gtggtactct ttccctggga acgttcagac tatgtaccga agatccgcaa ggcgttcggt
     4201 atcaggtgat cagaggtaag atttcagctc tgtgctggta gtggtctttc gtaaacgccg
     4261 gatggctctt tctttgatct gacgaaccct ctctttggtc aggtccaggc tttctgcaat
     4321 ctcttccagg ttcatttgtg gtgtaccatt gataccaaag tatgctgcaa tgatctctct
     4381 ttcacgatag ttgagcacat tcagaacctc ttcgatctct tttttgagtg aatctgatac
     4441 cagatcttta tcagggctgg aacgatcgtc tgtatcgctc atggtgtcca gcatactacc
     4501 ttcttcagaa tcacccaacg gcgcatccac agaaacatga cgatggtgac cacgggttat
     4561 atccttcacc tcatcttctt tcatgtgcat gatctctgcc agttcttcca gagttggctc
     4621 tctttcatat tcctgttcca gttgctgcga taatctgttc aacctggaat agctgcctac
     4681 tttattcagc ggcatacgca ccagcctggc ctgttctaca atagccagca tgatgctttg
     4741 cctgatccac caaacggcat aggaaatgaa cttgaagcct ttggtctcgt caaatctctt
     4801 agcagctttg atcagtccaa catttccttc attgatgaga tcagaaagag acaaaccctg
     4861 accctgatac tgcttggcta cagatacaac aaaacgaaga ttggctttgg tcagtttttc
     4921 cagcgcctca tcacttcctt cccggatctg cctggccagc gcagcctctt cttcaggtga
     4981 gataagatct atccttgaaa tttcgctgag atacttctcc aaagaatccg agtcacggga
     5041 tgtaatggag gcagtgatct tcagttggcg catcagagag gctggctttt actaaagata
     5101 cgaaagggct gctattttgt tgcagccctt tcaaaaggtt cagaaacttc acacgaagtt
     5161 tactcttcgt tcttggcagg agcctgcgga cgttccatca gcactttacg cgacagtctg
     5221 aactttccgg tcttcttatc tacctccagc aattttactt tcactttttc accttctttc
     5281 agcacaccct ccatactggg aaggcgggtc cagctgatct cagagatgtg aagcaggcct
     5341 tcctttcctg gcaggaattc tacgaatgcc ccgaagtcaa ctatcttctt gacggtgcct
     5401 tcatactctt cacctacttc cggcatggca gttatacctc tgatgcgagc cttggctgcc
     5461 tcaatgctgg ccttgtcagg agaggcgata tcaatgatcc cgaattcacc tacttcttcg
     5521 atgttgatgg tcgtgttggt ttcacgctgc atctcctgga tgatcttacc tccaggtccg
     5581 atcactgctc cgatgaactc tctcggtatg gtcatcttct caattcgcgg taccataggc
     5641 ttgtaatctt ccctcggtgc agtgatggtc ttggccatct cgcccaggat gtgcagcctt
     5701 ccgtttctcg cctggtccag agctttttcc agcacttcat agttcagccc atctactttg
     5761 atgtccatct gggtagcggt gataccatcg gcagtaccgg tcactttgaa gtccatatca
     5821 cccaggtggt cttcatcgcc caggatgtca gacaatacag catagcgggt catgcccgga
     5881 tcagagatca gacccatagc gatacctgca acaggcttct tcatctgcac accggcatcc
     5941 atcagcgcca tacttccggc acaaacagtg gccatggaag aagacccgtt ggattccagg
     6001 atctctgata ctacacgaat ggtataagga ttctcttcgg gcagcatatt cttcaatgca
     6061 cggagcgcca ggttaccatg gccgatctcc ctgcgaccag ttcctcttag tggacgggcc
     6121 tcaccagttg aatagggagg gaagttatag tggagcagaa atttgctttc accttcgaag
     6181 accacaccat cgatcagctg tgaatccagc ttggtaccga gggtaacgga agtgagtgac
     6241 tgtgtttcac cacgggtgaa tactgccgat ccgtgtacgg aaggaaggta gtccacttcg
     6301 gtccagatcg ggcggatctg atccagtgca cgaccatcca gacgcttgtg gtcagccagg
     6361 atcatttcac gcatgacttc ccattccagg tcgtggtaat aacgtttggc cagaccttcc
     6421 ttctcagcac gctcttcttc agacatcgtt tcaatgtact ctgtgatgat ggctttgaag
     6481 gcatctgatc tttcctgttt ggctgttccg ccgtaagcca cagcctttac tttctcacga
     6541 aggttgctca tgatggtatc gcgtagcgct tcatcatgta cttcgtggct gtaggtacgt
     6601 ttggtgtcct tacccagttc tgactgcaaa ctgagcagca gttcacattg cctgcggatg
     6661 gtttcatgac caaacttgat ggcgccgagc atatcttttt cgctcacttc cttcatctca
     6721 ccttccacca tcatgatatt atcaaccgta ccggcaatga tcatgtccat atcgctttgc
     6781 tccatggcag aacgggtggg gttgatcaca tattcgccat tgatgcgacc cacgcgaact
     6841 tcactgacag gacctccgaa tggaatgtca ctgcaggcaa gtgcagcaga tgcagcaaaa
     6901 cctgccagtg catcaggcag aatttcttta tccagagaaa tcaggttaac gatcacctgg
     6961 gtatcagcat gatagtcttc aggaaacagc ggacgcaaag cccggtctat aaggcggctg
     7021 atcaggatct catactctga aggtcgggcc tctcttttca ggaagccacc gggaaaacgg
     7081 ccggcagcag cgtacatttc acggtactca actgtaagtg gcataaaatc aatgccttcc
     7141 ttggcttctt tggcagaaac tacagttgcc agcagcatgg tatcacccat gcgaatcact
     7201 acggaaccat cggcttgttt ggcgagcttt ccggtttcca gcatcatggt tctgccgtcg
     7261 ccaaaatcaa atgttttttg aatcggattc attgttaggt attgtataaa aagaaaaagg
     7321 gctatgcaga tactacttcc accacatagc cctttgcaat aaggtataga ttatcgtctg
     7381 atgttcagcc tggcgatgag cgcccgatat ccttcgatat ccttcttcat cagataattc
     7441 aggaaacgac gacgcttacc taccagcatc agcaagctgc gcttggtggc atgatctttc
     7501 ttgttggcct ggaggtgaga ggtgagcata ttgatacgct ctgtaaatat ggcgatctgc
     7561 gattcaacag aaccggtgtt cttttcagag ccgccaaact cctttacgat ctctttcttt
     7621 ctttctgcag ttactggcat tggaatacgt acattttaag gcatgcaaag ataccatcat
     7681 ttgccgaaaa tgcaaatagg caagccctga gagcagagaa ttatttaccg gtatccggca
     7741 tcatgtaagc cagcagccgg gccatttcgc tgcgcaggtc tttgcgttct acgatcaggt
     7801 ccaggaagcc ggtctccagc acgaattcag cctgctggaa gccctcaggg aggtcttttt
     7861 taatggtttc ctttactact ctggggccgg caaagcctat aagtgccttt ggttcagcca
     7921 tattgatgtc gcccagcatg gcataactgg cggtcacccc gcctgtagtg gggtctgtca
     7981 taaaggagat ataaggtaac ccgtgcctgg ccagctgggt cagtttggcg ctggtcttgg
     8041 ccatctgcat cagcgagaat ccggattcca tcatgcgggc tccaccagat ttggagatga
     8101 tgataaatgg caatttgtga gaaatgctat agtcaatacc cctgctgatc cgctctccta
     8161 ccacggttcc catagacccc ccgatgaaag gaaaattcat acaggctatc accactccct
     8221 gtccttcaat ggttccgtga cctacactga tggcatcaga atagcccgat ttcttccggg
     8281 cttcgtcaag gcgtttggag tagggtttga gatccacaaa acccagtgca tcggtggaaa
     8341 tgatctcttc aaacagcagt ttgggttctt catcaaacag gatctgaaaa tattcatgag
     8401 aacctaccct tccgtggtac ccacaattcg ggcagcacca tagatggtct tcataatctg
     8461 tggtggtagt ggtcttttta cagtcagggc atttgaacca aaccccttcc ggtgtatcct
     8521 ttttctctcg ggtggaggtt tggatacctt cccgcattct cttgaaccaa ctcacgttgc
     8581 agtgtttaag gacaggcagc ggcggagatc atccgtggct gccggacaaa tgtaatgatt
     8641 caaccgaata tcaggcgatg gtgacttcgt tgctgagata tacgtcctgg atggcgttca
     8701 gaatgcctac accctctttc atggggcgct ggaaggcctt ccgaccactt atgagaccca
     8761 taccgcctgc acgcttgttc accactgcgg tagctactgc ctcagccagg tcactggcac
     8821 cggaagatgc acctccggag ttgatgagac ctgccctgcc catataacaa ttggccacct
     8881 ggtagcggca caggtcaatg ggatggtctg tggtcagctc agtatagatg cgcttatcat
     8941 tcttgccgta agcagattcc ttggtgttca gtgccaggta accgccgttg ttctccggca
     9001 gtttctgctt gatgatatct gcttcaatgg tcacaccgag gtggttagcc tgtccggtca
     9061 ggtcggcact cacatgatag tcaacaccat ctttcttgaa tcccgggtta cggaggtaac
     9121 accacagaat agtagccata cccagttcgt gggcgtattc aaaagccctc gctacttcct
     9181 gaagctggcg ggtgctttca tcagatccga agtagatggt cgctcctacg gccacagcac
     9241 ccagatccca gctggccttg atggaggcga acatgatctg gtcgaattta ttgggatagc
     9301 tcagaaattc gttgtggttg atctttacga tgaaagggat cttatgtgcg tatttacggc
     9361 tcacggttcc gagtgcaccg aaagtggtag caacggcatt acatccacct tctataccca
     9421 gtttgatgat attttcgggg tcgaaataga tcgggttggc ggcaaatgat gctccggcac
     9481 tgtgctcaat tccctgatcg accgggagta tgctcaggta accggagttg gccagcctgc
     9541 cattgccaaa taatgtctgc aggctgcgca gcacctgggg gttgcggtcg ctctgcatcc
     9601 agacacgatc tacaaaatcg ttaccgggta ggtgaagatc ttttttgctg atggtcttac
     9661 actcatggtt gagcaggtat tcggctttct cgcccaacag actgctgata ttactgatag
     9721 acataattgt ttgctttagg tttcgggtac gggcaaagat aagatgcacc aaccggatgc
     9781 aaaagaaaac gggcaccgca agcggtgccc gttgaaaatc agttgtttct gttattctgc
     9841 gaagtccagt tcacccatga tgtgctggca gttagccatc ttatccacga tgaacagtac
     9901 atagcggatg tccacattga tggtgcggtt catcttcgga tcgaaaacat aatcgcccgg
     9961 agtaccctca taattgccat cgaatgccag accgatcatg tcgccacggc ggttcatgat
    10021 ggggcttccg ctgtttccgc cggtaatgtc attatcagac aggaaacaaa cgggtacatc
    10081 tccggtctca tcggcatacc tgccaaaatc acccgctttg taaaggttga acagggcatc
    10141 gggtaccatg aattcttcgt cgttgttatc catcttctcg atgacgccat ccaggtaggt
    10201 ctgccagtgg taattcacgg catctttcgg actgtaatcc caaaccttac cgtaggtcag
    10261 tcgcatgctg aagttggcat ccggatagaa ggccttgtca ggatgcatct tcatggtagc
    10321 agctgtatat tcccggttga gtcgggacag ctcacgacga ttacccctga aatctgccag
    10381 cttggccaga ccttcttcat tgatattggt gaacagcctg tacacctgat cgttctgcag
    10441 ggtcttcagt ttgggcttgg ccaggaaggc attcagcctg gattcgctgg tcacaatact
    10501 ggtagcaaaa gcattctttg cccagttggt gatcttttca tcatcattca tgccctgagc
    10561 agttgccagg atattcttga gcgattcggg cttctgtgct tcaggcaggt tgttgttcat
    10621 gacgatcagc atggaggtga ccacatcacg ttcaatgttg ccgtacatgg tgggccatgc
    10681 ttcctgaccg gctgcctgga tctcggcgat cacggcatcc ttgtcttcct tagatgcttt
    10741 cttgtctgcc agcagcggct ccagcccctg cagggtcaga gcgataccgg ccgtggaagg
    10801 cagaatgacc gcatagcgct tataatagaa ttcccggttg atggcactca gtgcgccata
    10861 tacagaggcg tattcatcca tcaggccgcc atacatctct tgggtgttct tatcctgacg
    10921 agccagccag ttattaaatg ctttttcttc agcttctttt acagccaccg catccagtac
    10981 acgggtcata ccttctattt gcccggtata gagtttaagc acattcatgt agctggcata
    11041 atcaggagaa agttccaggc gaacggcatc actcttgtcc atttccactt tcatggcatc
    11101 tgtaactgcc ttgaacatgt ctatctgggc cggattggtc tcatagatga cttctttcat
    11161 ggcccagctg gtcaggtaac ggttggtaga gccgggaaag cccatgatca tggcaggatc
    11221 accttcctgg ataccgctga tatcgatctt gaggtggcgc ttaggatgat agggcttgtt
    11281 gttttcgctg tattcagcag gttcattgct ggcatctgca tagacacgga agatgctgaa
    11341 atcgccggta tgacgaggcc attcccagtt gtcagtatct ccgccatact ttccgataga
    11401 ctgtggaggt acacccacca gacgcacatc attgtaacgt ttgcggtaga agatgtaata
    11461 ctggttgccg ttgtacatac ttttgatctc tacgcccagg cctgaagaaa cttcttcacg
    11521 ggcagtgatc cggtctatga tctcacgtac cttcatgcca cgtgcactct cgtccaggtc
    11581 tttaaccaat ggcaggatgg aatctgtaat gttatcggca cgaaccagta attcaatata
    11641 caaaccctct atcgggatct cctcatcata ggagcctgcc cagaatccgt tgtccaggta
    11701 attgtgttcg gtagtgcttg ctccggccac cgcatcataa ccgcaatggt ggttagtgaa
    11761 taccaggccg ttggcagaca cgaattcgcc ggtacaaaaa ccgccaaata ccattacggc
    11821 atctttcaga gaaggcttat ccaggctgta gatatcttca gcttccagct caaagcctat
    11881 cttcttcatc tcttggatgt tcatattggt gatcatggag accagccaca taccttcatc
    11941 aggagcgggg aagggacccg aggcgctgac actgctcacg ccgatcacag ctgtaagcac
    12001 cagcatgttg atttttctaa atatcatgat acgtgtttaa ttaagtgcta agtaagagcg
    12061 cacaaatata agaagggagg ggctaagaaa cgagtaatga cagcttacag ctccttcatg
    12121 aagcgcttgg tgagcaggtt ctttccctgc gattgtgcgc tgaggaaata gaccccgggt
    12181 tgcagttcac tgaggtctat cttcaggaaa ttggttcctg attgtacttc tactgtcagc
    12241 acctcacgac ctacgatgtt catcacagaa atgcgttcca catctgctga gaaggagatg
    12301 gtagcctctg ttttgaccgg attggggaaa atggccagag aagtctgacc atcaacatgg
    12361 gaagcaacgt caggctctcc ttccgggcgc acggctgaaa aggcctgaag gctacccaaa
    12421 agaaacacag acaataagag agcgtacaga ttcttcaata gctgacgttt acactgtaaa
    12481 aataataatg ttctacgtaa tccttcaaat tattgtttcc acatgtgaca tcttaacagg
    12541 gtttaactgc ttcagaagtc atcatcttcg tcttcttctt cccagtcttc gctgtcatca
    12601 gactggaaca tagcacttag cagatcgccg aaaacagtgg catgctgcag gatctgcttg
    12661 ttcagtctgg aatactctgc cagcccgtgc aggacgaatt ccatcagaaa ataatgttca
    12721 tcgggtgaca cctgcgggta gtaattgctc accagatcag acaaaccggt cactttatca
    12781 agtgccttac ggtattcggt ctccggcagg tcatacagca ggtccagctg gtggccactg
    12841 ttgaaccagt caacaacgga tgcataatcg gcaggaatat ctgccccctc ttccctgccc
    12901 ttcccaagtg gttttctgcc ggattcgggt gccggaaaat aagacgggaa tctgttccgc
    12961 accgctttcc ctatcaggtt cagcgctaca ataaaaggac cttcctgttc accctcatac
    13021 accagttcca ctttaccggt aatggagggg atgacgcccc agaaatctga catacgcacc
    13081 caggtaagtg attcaccatg ccttaatgct cttctttctg cagcacttac aaggttttcc
    13141 agtgcggtga tggtcatgcg ggcagacacg ccacttttct ggtccacgta ttcgcttttg
    13201 cgggcttcca tggcaatggc ctctaccagt tcttctgcaa taccgcccag tttgaccaga
    13261 gcttcctgat ccgggtgggt tcgggcttcc tgtcgggtga tgcgtctgct ggtctcgaga
    13321 tcttttggat agtgggtcag gatctgactc tcaatacggt ctttcagcgg ggtgatgatg
    13381 gcacctcggt tggtatagtc ttccgggttg gcggtgaaca cgaaagtgat gtcaagcggc
    13441 atgcgcaccc ggaaaccgcg tatctggata tcaccttcct gcagcatatt gaacagagcc
    13501 acctgtatcc ttgcctggag atcgggcagt tcattgatca cgaaaatgca ccggtgtgat
    13561 ctcggtatca ggccgaagtg gatgacccgt tcatctgagt aaggtacttt gagcgttgct
    13621 gctcgaatag gatctacatc acctataaga tctgccacag acacatcagg ggtggccagt
    13681 ttttcggtga accgatcatt gcggtgcagc cattctattg gcgtgtcatc gcctttttca
    13741 gcaatgaggt ctttcgcata acgcgagatg ggatccatgg gatcatcgtt cagttcacta
    13801 cctgccacca caggaatata ttcatccagc agttccacca tgcgccgtgc aatgcgggtc
    13861 tttgcttgcc cgcgtaaacc cagcagcaac atgttgtgcc tggataagat ggctctttcc
    13921 agatcaggaa tgaccgtatg ctcaaaacca tagatccctt cgaataaagg ctcaccactg
    13981 cgcagtttcg ttatcaggtt gctgcgcaat tcttccttga tgcttctggg ccggtagccg
    14041 ctcttcttta atgcgcctaa tgtgttgggt ctggacatgt tcgttattcg ttgtgcgttg
    14101 atcgttgttc gttgtgcgtt attcgttctg cttagtcgtt ataaattgct atcccactct
    14161 cactcccact ctcactccca ctcctcactc tc
//
LOCUS       DAOMAX010000047        14008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_512_length_14008_cov_3.918655, whole genome shotgun sequence.
ACCESSION   DAOMAX010000047 DAOMAX010000000
VERSION     DAOMAX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_512_length_14008_cov_3.918655"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4327)
                     /locus_tag="PK742_04180"
     CDS             complement(<1..4327)
                     /locus_tag="PK742_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75819.1"
                     /translation="MKRILPLVLLLTVGFSVLAQPQEFSYNHEDFLSELGPFMRANGN
                     EEGRLAFEEFKNQMEKKNLTDLQVEQFIDLCNAMLGKKIRPNPAFTNLCDATVAFILA
                     GRLPDQFTDWMAVSTAVLMERKSADFIRFLEFSESFYGQNALHKTPGKEWTYYSYDFK
                     LTQEEGIPIVQFMDVSLQGTSTNDRAGIYETKGNYYPLDKTFKGSGGRINWAKVTLDS
                     LNVFAELPASWQISLEKAEYSIDSAVFYFIGYLEKPLEGTVKDKIFANVTDPEAVKYP
                     QFQSYDNSIRIKDILDRVDYYGAFSIRGKDIEGFGAGNDLATLKFRDDYDSEVMTTRA
                     KNYLIKQDAIFSSSAAVTIHMGDDSIYHADANLNYQADKRTVTITRGKIGAAGGDYYS
                     SYHRIDANLEQIIWNINDTSLIMRNMRGAGQKSAYFESEDLFSMELYQMIQGIAPNNP
                     IVMIKRYCERNDVREVPADIIAREINPSLTIDGIRSLLYQMMLEGFLFYDPDTDMVTV
                     KDKTFNYTNAYTGRQDYDIISMLSQVDGNNAELDLVKYELHLHGIEQVYLSDSQFVLI
                     YPDEGNILMRKNRDMLYGGRTVAGLVDFISKNFYFDYDTFDIKMNAVDSMIMFVESTE
                     SDNMGNVLYLPIKTSLSVTDGKLQIDRADNKSSRKDFSWYPVFTAYSKSKADYQKKEV
                     FNNVYKKDRFYFEVDPFTIDSLDIVNWAKEEFAGTMVSDGIFPEFREALTLQKDRSLG
                     FRTTTPGGGFAMYDGKGTFKDSVFLSNEGFRGNGTIEFMASRSRSNDFLFFPDSTLGK
                     VQDFTVLRTGGDIEFPSVLNSNVDMVWLPGKDVMHVRQGETPFDFFDGIASMRGDLRV
                     TSKGLWGDGTISWMEADLTSRELFFGEQDIFADSAGLTIKSIREGVFALKMDDINAEV
                     SFETQHGIFRSNNDTVLTRMPYNMYKTTINEFDWDMANRVIDFKSTNDDYGTFYSMEP
                     TQDGLNFEGNIGRLDLNTNVLEIGGIPHIGIADSWIIPDSGKVTIDADAKMRTLHNAT
                     ILGDSIFAYHTIYNATLDIWGRNSLKGNGFYKYISPQKKEQEIPLENITVIKKVDSLE
                     GYADYHIKALGYVNDSMPIELEEKLLFKGPVTLTMTDEHMRFLGYARVMLEDTLFPTS
                     WFRIDDLIDPNNPEFGINIAVTDKGDSVYAGVLRSFDSTNLYVRIMGEKVRKPDPVVF
                     SARGTGVYDEKAGMWYFGNRDKAIDPSLPGSLMTYNEKTGNVEGIGVMRLGFETDIVE
                     TRSYGKVVKMYDKNTYEFTNTIAIQLPLIESHMEKLSELLYAGNIENPPVEAYDNPLL
                     LASLANLGDERDVSRMLSSVEQLGYLSKPKEVKYNLLLSNLPMTWNEVSKTFHSTAST
                     GQIIWFGEQQFNQEIKVYAEFSKKRGGEMFTIYMETPYEDYVYIRCRKGQMRVYTSSD
                     EINEDI"
     gene            complement(4389..5225)
                     /gene="prmA"
                     /locus_tag="PK742_04185"
     CDS             complement(4389..5225)
                     /gene="prmA"
                     /locus_tag="PK742_04185"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQU75820.1"
                     /translation="MNYLEFTFTLEDQEMQDILIALLAEMGFDTFDETGHLLLAYTDA
                     DAFDEARTEALNTLCAQLNCSYRYTTMADKNWNEEWEKQFQPVIIENRCIIRAPFHQA
                     DDAYEQEIIIAPRMAFGTGHHASTYLMVQAMMDMDLREKTICDAGTGTGVLAILAAKL
                     GATDILALDNNEWAYNNALDNLALNDVLEVVDMQLAEPPLIQDKKFDVILANINRSVI
                     LEYMELFRHALNDRGTLLLSGILTADMPLITRAAARAGLQEVRNLTREDWSCVEYTIH
                     AE"
     gene            complement(5222..5977)
                     /gene="tpiA"
                     /locus_tag="PK742_04190"
     CDS             complement(5222..5977)
                     /gene="tpiA"
                     /locus_tag="PK742_04190"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQU75821.1"
                     /translation="MRKKIAAGNWKMHKTLQEAEQTASALEAGITNDPACEVWIAPPA
                     PYLATLAATFKKMTWGSQDLSQHDSGAFTGEWSSEMLHSCGAGFSLIAHSERRQYHRE
                     DDVLAHQKILAALRHELTAVYCCGEHLSDREANDHFRIVENQIREALLALSHDAMGHV
                     VIAYEPVWAIGTGVTATTEQAGEMHRFIRSTLADMFGDHIAASARILYGGSVKPGNAA
                     ELFSDPDIDGGLVGGASLQADDFLAIIQAASAS"
     gene            complement(5979..7160)
                     /locus_tag="PK742_04195"
     CDS             complement(5979..7160)
                     /locus_tag="PK742_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HQU75822.1"
                     /translation="MGYPPVVLFDDDRYGQLLPLTFTRPAACLRSGILTQAERWEKLT
                     GQEPGYLTSPVLQNKYRNKTGAALYINGRLLADETAMKELLALQPGQSIWQEQQLIAC
                     YSTDTISSAEQLTGVEKADISVVTYTLIQRPWDLFSMAKTWITADFLWITSGKQSAPV
                     SATNTVIGKDLFIEPGAQVEAAVINTTSGPVYIGKDAEVMEGSMIRGPFALGDHSTLK
                     MGAKIYGATSIGPHCKVGGEVNNSMIFGFSNKAHDGFLGNSVIGEWCNLGADTNNSNL
                     KNNYAEVRMYDYLTGRFIHTGLQFCGLIMGDHSKCGINTMFNTGTVVGVNANIFGDGF
                     PRNFIPSFSWGGAAGFTTYKLADALTVAEAVMQRRGLQLNQTDKDILTYIFESTRAQR
                     Q"
     gene            complement(7221..7469)
                     /locus_tag="PK742_04200"
     CDS             complement(7221..7469)
                     /locus_tag="PK742_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HQU75823.1"
                     /translation="MKKDIHPKEYRTVVFKDFSSDYAFLSKSAANTKETIVWEDGNEY
                     PLVKLEISHMSHPFFTGKMKFVDTAGRIEKFNKKYGKK"
     gene            7575..8045
                     /locus_tag="PK742_04205"
     CDS             7575..8045
                     /locus_tag="PK742_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HQU75824.1"
                     /translation="MHWRKLNGEPIRVPIAQKVEDAILHETALGNRLKVCIGTDSQVY
                     GNDIEFATVIVFLREGRGGFMYIASQTLRQRMGLKERLLHEVTLSIQVAYSICDLLDR
                     YRVDLEVHADINTNPGFKSNTALSEAMGYILSMGFVFKAKPDSFASTSCANKVV"
     gene            complement(8042..8632)
                     /gene="yihA"
                     /locus_tag="PK742_04210"
     CDS             complement(8042..8632)
                     /gene="yihA"
                     /locus_tag="PK742_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQU75825.1"
                     /translation="MESVQAEYKGSYSKVDECPDPGLPEMAFIGRSNVGKSSLVNAIC
                     LQKNLAYISSNPGKTQSINFYLLNEVLYLVDLPGYGYARVSRTQRSAWEKMVRGYLEQ
                     RTSLFCVFQLVDASIPPQQADLDFTNWLGEQGLPVTLVFTKTDKRKKDKAAGAQAYLK
                     KLSEFWEQLPPHLSTSSTTGAGVDLLRKYMQELLPV"
     gene            8700..9425
                     /gene="ubiE"
                     /locus_tag="PK742_04215"
     CDS             8700..9425
                     /gene="ubiE"
                     /locus_tag="PK742_04215"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQU75826.1"
                     /translation="MHDQITPYQSDATKKEQVRNMFDNIAPKYDLLNRMLSLGIDQYW
                     RRSALKKLRQYQPDHVLDVACGTGDFSIAALKYGVHEVTAVDLSAEMLSKGRDKVHSK
                     GLESKIKMVQGDSEALAFPDDTFDAATVAFGVRNFEHLEAGLRDIRRVLKPGAPLLIL
                     EFSRPKVFPVKQLFQFYFRTICPLVGRLVSGDGKAYTYLYESVQAFPEREQMADILQQ
                     QGYRSVQWRTYTFGICALYIGEK"
     gene            9398..10102
                     /locus_tag="PK742_04220"
     CDS             9398..10102
                     /locus_tag="PK742_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU75827.1"
                     /translation="MRIVYWRKIVCILLLLLPFMAKAQFHVKEYEDRFARRPYHFGIG
                     LSYNSSYFKIRYDQSFINNDTLLTASALSGPGFNVSIVSNLRLTKHLDLRFIPGLAFA
                     EKVIQYDQVQDAATSQRIESIYFNFPLSVKFKSDSYKDMKVYVLGGTTYSFDLASNAK
                     ARNAEDIVKIGTHDVSLDYGFGFEFYFPYFIFAPEIKICNGLNNVHVRDPLLIYSSSL
                     DKLYARTILFTIHLEG"
     gene            complement(10103..10639)
                     /gene="dcd"
                     /locus_tag="PK742_04225"
     CDS             complement(10103..10639)
                     /gene="dcd"
                     /locus_tag="PK742_04225"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQU75828.1"
                     /translation="MILSDRRIREEMEKGSILIEPYRPECLGTNSYDVHLGRYLAVYS
                     SQILDARKHNAIEELIIPDDGLVIHPGVLYLGVTEEYTETHAHVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNTWTLEISCTQPVRIYAGMPIGQLIYFEVAGDIDNFYNKKKNAK
                     YVNRTIRPVESMMFMNDF"
     gene            complement(10644..11558)
                     /locus_tag="PK742_04230"
     CDS             complement(10644..11558)
                     /locus_tag="PK742_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75829.1"
                     /translation="MLLPAQIEVSLDATLRGHRAAVYAIDRTPEGDEVFSGAGDGMIA
                     RWLPESSADGEAVARLDSHIFSLLYLSESGSLIAGDMQGNIHFLPANNPDDFRQTSLN
                     SHAVYSMVRWQDDVFAGCGNGYIHRLSADSAEVLQSVQISGRHIRCLYIVNDLLYAGC
                     SDNCIYLLDPFDLSATAKQTGHQGSVFSLAFNPYLNQLLSGSMDAQVRIWTPDLDPVA
                     ALEAHLFTVNAIAVHPEWPIFATASRDKNIRIWDASNYTLLKVLTHDRYDGHTHSVNK
                     IFWSGSGGHLISCSDDRTLKVWDVRINL"
     gene            complement(11564..11995)
                     /locus_tag="PK742_04235"
     CDS             complement(11564..11995)
                     /locus_tag="PK742_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75830.1"
                     /translation="MSLDAILWLGLSSGICSSLLFFLSGRLLLSRLSRELPLQLQSLV
                     DDESVQRSIKDKFDDFVRLRLGQALPVLNMFIDDKLIEELKPVFMEEVTRGIPDLAGN
                     DRIARKMRNILSARMQKISVRLIPLWVTAHILCAYFFYSIY"
     gene            complement(11992..12633)
                     /gene="pyrE"
                     /locus_tag="PK742_04240"
     CDS             complement(11992..12633)
                     /gene="pyrE"
                     /locus_tag="PK742_04240"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQU75831.1"
                     /translation="MLLNASAASDFAAKLLQIQAIKLQPDKPFTWASGWYSPIYCDNR
                     MTLSYPDIRKDIARWLQEIRAAYFADSAVIAGVATAGIAHGLLLAEADSLPFIYVRSD
                     AKKHGMQNRIEGRLQPGDKVLVIEDLISTGGSSLQAVEALRQAGADVVGLAALFTYDF
                     EHARKAFEQANCPFCTITNYPSVVQAALSKDLIQADQEALLNTWREDPENWGK"
     gene            12632..13279
                     /locus_tag="PK742_04245"
     CDS             12632..13279
                     /locus_tag="PK742_04245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQU75832.1"
                     /translation="MAQIYKIFVNEIPVTILEGNPSDLPETIDEHRPVYFFTERDKIR
                     KAFALAETGNYVRSLTIIGEDARQIRQTLFSAYRLIRAAGGVVMNQHRQILLIHRHGM
                     WDLPKGKAEAGEQMQRTALREVEEETGVKPLVIVQKLRPTYHTYVTVQKVRILKKTNW
                     YLMQHDGKGKLRPQEEEGIDSALWVSAEDLIPKMQGTYGNIRDVLDQALLTVYFS"
     gene            complement(13260..13730)
                     /gene="coaD"
                     /locus_tag="PK742_04250"
     CDS             complement(13260..13730)
                     /gene="coaD"
                     /locus_tag="PK742_04250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQU75833.1"
                     /translation="MNSRIAVFPGSFDPITIGHHDLIDRAAGLFDKLIIAIGVNGQKQ
                     SFFPLEKRLQWLQDLYGHRNNISVEAYEGLTVDFCARHQAGWIVRGVRNAMDFEYEKT
                     IAALNRSLENGPETIFLPASPQFSHISSTIVRELVRHGGPYAHLVPLVVTKSTQ"
     gene            complement(13727..>14008)
                     /locus_tag="PK742_04255"
     CDS             complement(13727..>14008)
                     /locus_tag="PK742_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HQU75834.1"
                     /translation="QAEEFGLDLQVMQMDVFEYIRSASASYSLIFAGPPYGLPNLDKI
                     PDLIMEKGLLASGGWLILEHNPNHHFDEHPNLKMKRNYGTTIFSIFEMP"
BASE COUNT         3412 a         3330 c         3480 g         3786 t
ORIGIN      
        1 agatgtcttc attgatctca tcgctggaag tatataccct catttgtcct tttctgcaac
       61 ggatatatac ataatcttca tatggtgttt ccatgtagat cgtaaacatt tcaccgcccc
      121 ttttcttgct gaattcagca tacactttga tctcctggtt gaactgctgc tcgccaaacc
      181 agatgatctg tccggtagag gcggtgctgt ggaaggtttt gctcacttcg ttccaggtca
      241 tcggcagatt agacagcagc aggttgtatt tcacctcttt aggctttgac aaatacccca
      301 gctgttctac cgaactgagc attcggctca cgtcgcgttc atcgcccaga ttggccagtg
      361 atgccagcag cagcggattg tcatacgcct cgacaggcgg gttttctatg tttcctgcat
      421 acagcagctc cgacagtttt tccatatgcg attcgatcag tggcaactgt atggcgatgg
      481 tgttggtaaa ttcatacgtg ttcttatcat acattttgac caccttgccg tatgaccttg
      541 tttccacaat atctgtttca aagccgagtc gcatgacgcc tatgccttcc acgtttccgg
      601 tcttttcatt gtatgtcatc agtgatcccg gaagcgatgg gtcaattgcc ttgtcgcggt
      661 ttccgaagta ccacataccg gctttttcat catagacacc tgtaccacgg gcagagaata
      721 ccaccgggtc aggctttctg accttttcgc ccatgatgcg cacatacagg ttagtactgt
      781 caaatgatcg aagcacgcct gcatatacag aatcgccttt atcagtgact gcaatattga
      841 taccaaactc cggattattg ggatcgatca ggtcatcaat gcggaaccat gaagtaggga
      901 agagggtgtc ttccagcatg acccgggcat aacccagaaa gcgcatatgt tcatcggtca
      961 tggtcagggt gacgggtcct ttgaacaaca gtttttcttc cagttctatt ggcatggaat
     1021 catttacata acccagcgct ttgatatggt agtctgcata tccttccagg ctgtcaacct
     1081 ttttgatgac ggtaatattt tccagcggaa tttcctgttc ctttttttgg ggggagatgt
     1141 atttgtagaa gccattccct ttgagggaat ttcttcccca tatatccagt gtggcattat
     1201 aaatggtgtg gtaagcaaag atggaatcac cgaggatggt tgcattgtgc agggtgcgca
     1261 tcttcgcatc tgcatcaatg gtcaccttgc cgctgtcagg tatgatccag gaatcagcga
     1321 taccgatatg tggtatgcca ccgatctcca gcacatttgt attgaggtcc agccgtccga
     1381 tatttccttc aaaattcagt ccgtcctgtg tgggttccat actgtagaag gtgccgtagt
     1441 catcgttggt tgacttaaag tcaatgacgc ggttggccat atcccagtca aactcgttga
     1501 tggtggtctt gtacatgttg tacggcatgc gggtgagcac ggtatcattg ttggaacgga
     1561 agataccatg ctgggtctca aagctgacct ctgcattgat atcatccatt ttaagggcga
     1621 atactccttc gcggatactt ttgatcgtaa gccctgcaga gtcagcaaaa atatcctgct
     1681 cgccaaagaa tagttctctg gaggtaaggt cagcttccat ccagctgatg gtaccatcgc
     1741 cccagagtcc tttgctggta acacgaaggt caccacgcat actggcgata ccgtcaaaga
     1801 agtcaaatgg tgtctcaccc tggcgcacat gcatcacatc tttgccgggc agccaaacca
     1861 tgtccacgtt gctattgaga acactgggaa attcaatatc gccacctgtg cgcaatacgg
     1921 tgaagtcctg cactttaccc agggtgctgt caggaaagaa gaggaagtcg ttggaacgtg
     1981 agcgggaggc catgaattca atggttccgt ttccacgaaa cccttcatta ctcagaaata
     2041 cactgtcttt aaaagttcct ttcccgtcgt acatggcaaa ccctcctccg ggggtggtgg
     2101 tcctgaagcc taatgaacgg tctttctgca gggtcagcgc ttctctgaat tccgggaaaa
     2161 ttccatcaga taccatggta cctgcaaatt cctccttggc ccagttgaca atgtccagac
     2221 tgtctatggt aaatggatcc acctcaaaat agaatctgtc ctttttatat acattgttaa
     2281 agacctcctt cttctggtaa tctgcctttg atttggaata ggcagtgaaa acaggatacc
     2341 aggaaaaatc cttcctgctc gatttgttat ctgcacggtc tatctgcagt tttccgtctg
     2401 ttacagacaa gcttgttttg ataggcagat aaagcacatt gcccatatta tcagattctg
     2461 tcgattccac gaacatgatc atggagtcaa ctgcattcat cttgatatca aacgtgtcat
     2521 agtcaaaata aaaattctta gaaatgaaat caacgaggcc tgcaactgtt cgcccgccgt
     2581 aaagcatgtc tctgttcttg cgcatcagaa tgtttccttc atctgggtag atgagtacga
     2641 actgactgtc gctcagatag acctgttcaa taccgtgcag gtgcagttca tacttgacga
     2701 ggtccagttc tgcgttgttt ccatccacct gagaaagcat ggagatgata tcatagtcct
     2761 gcctgcccgt gtatgcattg gtataattga atgttttgtc tttaacggtc accatgtcgg
     2821 tgtcggggtc atagaacaga aagccttcca gcatcatctg gtacagcagt gagcgaatgc
     2881 catcgatggt caggcttggg ttgatctctc tggcaatgat atcagccggt acttcccgca
     2941 catcattacg ttcacaatag cgttttatca tgacgatcgg gttgttgggc gcaatgcctt
     3001 gtatcatttg atacagttcc atactgaaga ggtcttcact ttcaaagtag gcgctttttt
     3061 gtccggctcc ccgcatgttt cgcataatca gagaggtgtc attgatattc catatgatct
     3121 gttccaggtt ggcatcaatg cggtggtagg agctgtagta atcgccaccg gctgcaccga
     3181 tcttacccct ggtgatggtg actgttcttt tatcagcctg gtagttgagg ttggcatctg
     3241 catgatagat ggagtcatcg cccatatgaa tggtaacggc agcgctgctg gagaagatcg
     3301 catcctgttt gatcaggtag tttttagctc tggtggtcat gacctcagag tcataatcat
     3361 ctctgaattt cagggtagcc agatcgttac cagcaccaaa gccttcaatg tcttttcccc
     3421 ggatggaaaa agctccgtag tagtccacac gatccagaat atccttgatg cgaatgctgt
     3481 tgtcatagga ctggaactgt gggtatttca ccgcttccgg gtctgttaca ttggcaaaga
     3541 tcttgtcttt cacagtgcct tcgagcggct tttccaggta acctatgaaa tagaaaacag
     3601 cactgtcaat ggagtattcg gctttttcca gactgatctg ccaggaggcg ggcagctcag
     3661 caaacacgtt caggctgtcc agtgtaactt ttgcccagtt gatacgtcca ccgctgcctt
     3721 tgaatgtttt atcgagcggg tagtaatttc ctttggtctc atatatgcct gcacggtcat
     3781 tggtagaagt accctgcaga ctaacgtcca tgaactgaac gatggggatg ccttcttcct
     3841 gggtcagctt aaaatcataa gagtaatagg tccattcttt accgggtgtt ttatggaggg
     3901 cgttttgtcc gtaaaatgat tcagagaatt ccagaaagcg tataaaatct gcagattttc
     3961 tttccataag aaccgctgtt gacacggcca tccagtcggt gaactgatca ggcagtctgc
     4021 cggccagaat gaatgccact gttgcgtcgc acaggttggt aaatgccgga ttggggcgga
     4081 tcttttttcc cagcatcgcg ttgcacaggt caatgaattg ttccacctgg aggtctgtca
     4141 gattcttttt ctccatctgg tttttgaact cttcaaaggc aagtcttcct tcttcattgc
     4201 cattggcccg catgaatggt ccgagttcag acaggaaatc ttcgtggttg taggagaact
     4261 cctgcggctg ggccaaaaca gagaaaccga cagtcagcag caataccaga ggcaggattc
     4321 ttttcatttc gtcaagttat gcaaaattga acacaaaaga gggtcataaa ttgtctgtta
     4381 acaggagctt attcagcatg aatggtgtat tccacgcatg accagtcttc tctggtgagg
     4441 ttgcgcactt cctgcaggcc ggcacgtgca gcagccctgg tgatgagcgg catatctgca
     4501 gtgaggatac cggatagcag caatgtaccc cggtcattca gggcgtgccg gaacagttcc
     4561 atatactcca gaataacaga gcggttgata ttggccagga tcacatcaaa ttttttgtcc
     4621 tgtataagcg ggggttcagc cagttgcata tccactactt ccagcacatc atttaatgcc
     4681 aggttgtcca gtgcattgtt ataggcccat tcattattat ccagggcgag gatatcagtg
     4741 gcgcccagtt tcgctgccag gatcgccagc acccccgttc cggtgcctgc atcgcagatg
     4801 gttttttcac gtaggtccat atccatcata gcctgaacca tcagataggt actggcgtgg
     4861 tgaccggtac caaatgccat tctgggggcg atgatgatct cctgttcgta agcgtcatca
     4921 gcctgatgaa agggagcacg aatgatgcac ctgttttcta tgatgaccgg ctgaaactgt
     4981 ttttcccatt cttcgttcca gttcttatcg gccatggtgg tgtacctgta gctgcagttg
     5041 agctgcgcac acagggtatt caatgcttcg gtgcgtgcct cgtcaaaggc gtcggcatct
     5101 gtataggcaa gcagcagatg gccggtttca tcaaaagtgt caaaccccat ttctgccagc
     5161 agtgctatca ggatatcctg catttcctgg tcttccaggg tgaatgtaaa ttccaggtaa
     5221 ttcatgaagc ggatgctgcc tgtatgatgg ccagaaaatc atcggcctgc agggaagcgc
     5281 cacccaccag gcctccatcg atatcgggat cactgaacaa ctcagcggca tttccgggtt
     5341 tgacactgcc tccatacagg atcctggcag atgcagcaat atgatcgcca aacatatcag
     5401 ccagtgtgct gcggatgaaa cggtgcattt cgccggcttg ctcagtggta gcggtaacac
     5461 ctgtgccgat ggcccagaca ggttcatagg caatgaccac atgacccatg gcatcatgac
     5521 tcagagcgag cagtgcctct cggatctgat tttccactat acgaaaatga tcatttgctt
     5581 ctctgtcaga caggtgttcg ccgcagcagt acacagctgt aagttcatga cgcagggctg
     5641 ccagtatttt ctggtgggcc agcacatcat cttcgcggtg gtactgacgt ctttcactgt
     5701 gagcaatcag tgaaaagcca gcaccacagg agtgcagcat ttctgaagac cactcgccgg
     5761 taaaagcacc agaatcgtgt tgactcagat cctgagaacc ccaggtcatt tttttgaacg
     5821 ttgctgccag ggttgccagg tatggagcgg gcggtgcgat ccatacctca caggcaggat
     5881 cattggtgat acctgcttcc agggctgatg ctgtttgttc agcttcctgt aaagtcttgt
     5941 gcattttcca gttccctgct gctatcttct ttctcatatc attgtctttg tgcacgtgtg
     6001 ctttcaaaaa tataggtgag tatgtcttta tcagtctggt tcagctgaag cccacggcgt
     6061 tgcatgaccg cttcggccac ggtcagggcg tctgccagtt tataagtggt gaatccggcg
     6121 gcacctcccc aggagaatga tgggatgaaa tttcgaggga atccatcgcc aaagatattg
     6181 gcatttactc ccaccaccgt tccggtattg aacatggtgt tgatgccgca tttggaatga
     6241 tctcccatga tcaaaccgca gaactgcagt ccggtatgaa tgaatcttcc cgtgaggtag
     6301 tcatacattc ttacttctgc ataattattc ttcaggttgc tgttattggt atctgccccg
     6361 aggttgcacc attcgcctat gaccgagttg cctaagaatc catcgtgggc tttattggag
     6421 aacccgaaga tcatactgtt attgacctcg ccacccacct tgcaatgcgg tccgatcgat
     6481 gtggcaccgt agatctttgc gcccattttg agggtgctgt gatcgcccag tgcaaaaggc
     6541 ccccggatca tagaaccttc cattacttcg gcatcctttc caatgtacac cggtccggat
     6601 gtggtattga tgactgcagc ttctacctgt gcaccaggtt caatgaacag gtcttttcct
     6661 atgaccgtat tggtagcaga gacgggagca ctttgtttgc ctgaggttat ccagaggaaa
     6721 tcagctgtga tccatgtttt tgccatactg aacagatccc atgggcgctg tatcagggta
     6781 taggtgacga ccgaaatatc tgctttttct acacctgtaa gttgctcagc agaagagatg
     6841 gtatcagtac tgtaacaggc tatcagctgt tgttcctgcc agatgctttg tccgggctga
     6901 agtgcaagaa gctctttcat ggcagtttca tcggccagca accttccgtt gatatacaat
     6961 gcggcgcccg tcttattccg atatttattc tgcaagacag gtgaggtcag atagcccggt
     7021 tcctgaccgg tcagtttttc ccatcgttca gcctgggtca gaatgccgct gcgcagacag
     7081 gctgcaggcc tggtgaaggt aaggggaagc aattgcccgt aacgatcatc atcgaagagc
     7141 acaacaggag ggtagcccat gtagcaaaaa taaaaaaccc cgccagtgta tgacggggtc
     7201 tatgcgatct tcggccggaa ttatttcttg ccgtactttt tgttgaattt ttcgatacga
     7261 ccggcggtgt ccacaaactt catctttccg gtaaagaaag ggtgagacat gtgagagatc
     7321 tccagtttta ccagggggta ttcattgcca tcttcccaaa caatggtttc tttggtattg
     7381 gcggcagact tactcagaaa tgcatagtcg ctggaaaagt ccttaaaaac gacagttctg
     7441 tattctttgg gatggatgtc ctttttcatt ttacggatgc aattgagggt gcaaatttaa
     7501 ttaaatttca ggaaacacta agtgaaagtg atttatttac ttttaggaat cgtttaaagt
     7561 acactaaaat ggacatgcac tggcgaaagc tcaacggaga acctataagg gttcctatag
     7621 cacagaaggt ggaagatgcc atcctgcatg aaacggccct gggcaacagg ctaaaggtat
     7681 gtattggcac agattctcag gtgtatggga atgacattga gtttgccacc gtcatcgttt
     7741 ttctgcggga aggcaggggc ggattcatgt atatagccag ccagaccctg aggcagcgta
     7801 tgggtcttaa agagcgcctg ttgcatgagg tgacgctcag tattcaggta gcttatagca
     7861 tatgtgacct tctggaccgg tacagggtgg atctggaggt gcatgcagac atcaacacca
     7921 atccgggatt caagagcaat acagcactta gtgaggccat gggatacata ctcagcatgg
     7981 gtttcgtgtt taaggccaaa cctgattcat ttgccagtac cagctgtgcc aataaggtgg
     8041 tctaaacagg aagcagttcc tgcatgtact ttctgagtag atctactccg gcaccggtgg
     8101 tggatgaagt actcaggtga ggaggaagct gctcccagaa ttcgctcagt ttcttcaggt
     8161 aggcctgagc ccctgccgct ttatctttct tgcgtttgtc tgtcttggta aagaccaggg
     8221 ttaccggaag tccttgttcg cccagccagt tggtgaaatc aagatcggcc tgttggggtg
     8281 gaatggaagc atcaaccagc tgaaaaacac aaaaaaggga ggtgcgttgc tccagatagc
     8341 ctctgaccat cttctcccat gcgcttcgtt gggttctgga aacccttgca taaccgtagc
     8401 ccggcagatc aacgaggtaa agtacctcat tgagcaggta gaagttgatg ctctgtgttt
     8461 tgccgggatt ggaggagata tatgccagat ttttttgcag acagatggca ttgaccaggc
     8521 tggacttgcc cacattactt cgcccgataa aggccatttc cggaagtccc ggatccggac
     8581 attcatccac tttgctgtag ctgcctttgt attctgcctg tacactttcc acgcaacaaa
     8641 actacaacct gtctgttgga gtagtctgaa ggggcaaata cttacctttg caggcgctca
     8701 tgcacgatca gatcacacct tatcagtcag atgccaccaa aaaggagcag gtgcgcaata
     8761 tgtttgacaa cattgcaccg aagtatgacc tgctgaaccg gatgttgtct ttgggcattg
     8821 atcagtactg gcgcagatct gccttgaaaa aactcaggca gtaccaaccc gatcatgtgc
     8881 tagatgtagc ctgcggaacg ggcgatttca gtattgctgc tctgaagtac ggagtgcatg
     8941 aagtgaccgc agtagatctg tcggctgaaa tgttatcaaa aggacgagac aaggttcact
     9001 caaaggggct ggagtcaaag atcaagatgg tgcagggcga ttctgaagcg ctggcctttc
     9061 ctgacgatac ttttgatgcc gctaccgtag ctttcggggt caggaatttt gagcatctgg
     9121 aagcaggact gagagacatc aggcgtgtgt tgaaaccggg tgctccgttg ctgatactgg
     9181 aattttccag gccgaaggtg tttcctgtaa aacaattgtt tcaattttat tttcgcacaa
     9241 tttgccctct ggtggggcgt ttggtgtctg gagacggtaa ggcctacacc tatctctatg
     9301 aatccgttca ggcctttccc gagcgtgaac agatggcaga tatcctgcaa cagcagggat
     9361 accggtctgt tcagtggaga acctatactt ttggtatatg cgcattgtat attggagaaa
     9421 aatagtatgt atcctgctgt tgctgctgcc atttatggct aaggcacagt ttcatgtaaa
     9481 ggaatatgag gacaggtttg cccgaagacc ttatcatttt ggtataggat tgtcttataa
     9541 ttccagctac ttcaagatcc ggtatgacca gagcttcatc aacaatgata ctctgcttac
     9601 agccagcgcc ctaagcggac ctggtttcaa cgtgagtatt gtgagtaatc tcaggctcac
     9661 caaacacctt gatctgcgat ttattccggg tctggccttt gctgaaaagg tcattcagta
     9721 tgatcaggta caggatgctg ccacctcgca gcgcattgag tccatttact tcaactttcc
     9781 cttgtctgtc aaattcaaga gtgattctta caaggacatg aaggtttatg tgctgggtgg
     9841 aaccacatac tcttttgacc tggccagtaa tgccaaagcc cggaatgccg aagatatcgt
     9901 caagatcggc acccatgatg tgagtctgga ctatgggttt gggtttgagt tctacttccc
     9961 ttacttcatt tttgctccgg agatcaagat ctgtaacggt ttgaacaacg tacacgtgcg
    10021 ggatccgctg ctcatttatt caagttcact ggacaagctc tatgccagga ccatcctgtt
    10081 caccatccac ctggaaggct gatcagaaat cattcatgaa catcatagat tcaaccggac
    10141 ggatggtacg gttgacatac ttggcattct tctttttgtt gtagaagtta tcgatatctc
    10201 cggccacctc aaagtaaatg agctgaccga tgggcatacc ggcataaatg cgcaccggct
    10261 gggtgcagga tatctccagg gtccaggtgt tgcagaagcc tacgtcgccc tttcctgcag
    10321 tggcatgaat atctattccg agtctgccca cactagattt tccctccagg aagggcacat
    10381 gggcatgggt ctctgtatat tcttctgtta cacccagata gagtacaccc gggtgaatga
    10441 ccaggccatc gtcaggaatg atcagttctt ctatagcgtt gtgtttccgg gcatccagga
    10501 tctgcgagct gtacactgcc aggtagcggc ccaggtgtac atcgtatgag ttggtaccca
    10561 ggcattccgg gcggtatggt tctatgagaa tggagccttt ctccatttct tctctgatac
    10621 gtctgtctga taagatcata ggtctataga ttgatccgga catcccaaac tttgagggtc
    10681 cggtcatcgg agcacgaaat taaatgcccc ccggaaccgc tccaaaagat tttattcaca
    10741 gaatgggtat gtccgtcata gcggtcatgt gtaagcacct tcaggagggt ataattgctg
    10801 gcatcccaga tgcgtatatt cttgtctctg cttgcggtag caaaaatggg ccattccggg
    10861 tgtactgcaa tggcattgac tgtaaacagg tgggcttcca gtgctgccac cggatccagg
    10921 tccggggtcc agatgcgcac ctgtgcatcc attgagcctg acaacagttg gttcaggtag
    10981 ggattaaaag ccagactaaa tacagagccc tgatgaccgg tctgttttgc ggttgcagac
    11041 aggtcaaacg ggtcaagcag gtaaatgcag ttatcgctgc atccggcata cagcaggtcg
    11101 ttcactatat acaggcagcg tatatgcctg ccggagattt gaacagactg gagaacttct
    11161 gcactatctg cactcaggcg gtgaatgtag ccgtttccgc acccagcaaa aacatcgtcc
    11221 tgccagcgca ccatcgaata taccgcatgg ctgtttaggg aagtttgtct gaaatcatct
    11281 gggttattgg caggcagaaa gtggatatta ccctgcatat ctccggctat cagactacct
    11341 gattcgctca ggtaaagcaa tgagaaaatg tggctgtcaa gccgggcaac tgcttctccg
    11401 tctgccgaag attcgggcag ccacctggca atcattccat cgcctgcacc gctgaaaact
    11461 tcatctcctt ccggagtgcg gtcaatggca tataccgcag ctctgtgtcc tctcagagtg
    11521 gcatcaagtg aaacctcaat ttgagccggt aaaagcatgt ttatcagtag attgaataaa
    11581 aaaagtatgc gcacaaaata tgagcagtta cccaaagtgg aataagccgg acagagattt
    11641 tctgcatcct ggcagacagg atattccgca ttttccgggc tattctatcg ttgcctgcca
    11701 ggtctggtat tccgcgggta acttcttcca taaagaccgg cttcagttct tcgatgagct
    11761 tatcgtcaat gaacatattc agcaccggca gtgcctgtcc cagtcttaaa cgcacaaaat
    11821 cgtcgaattt gtctttgatg gatcgttgca cgctttcatc atccaccaga gattgcagct
    11881 ggagcggcag ctcgcggctc aaacggctga gaagtaatcg gccactgaga aagaacagca
    11941 ggctgctaca aattccggaa gacagaccaa gccagagaat ggcatccagg ctcattttcc
    12001 ccagttttcc gggtcttcac gccaggtatt cagcaatgct tcctggtctg cctgaatcag
    12061 atccttgctc agtgcggcct gcactacaga agggtaattg gtgatggtac agaaagggca
    12121 gtttgcctgt tcaaaggctt tgcgggcatg ttcaaaatca taggtgaaca aggcagccaa
    12181 acccactaca tcggctccgg cctgtctcaa agcctccact gcctgcaggc tgcttcctcc
    12241 tgtagaaatg agatcttcta tcaccagtac tttgtcgccc ggttgcaatc gtccttcaat
    12301 acggttttgc ataccatgct ttttggcatc agagcgcaca taaatgaacg gcagagagtc
    12361 tgcttcagcc agcaggagtc cgtgtgcgat acctgcggta gctacccctg caatcacggc
    12421 cgaatctgca aaatatgcgg ctctgatttc ctgtaaccat cttgcaatat cctttctgat
    12481 atcgggatag gagagggtca tccggttgtc gcagtagatg ggcgaatacc atccggaagc
    12541 ccatgtaaat ggcttgtcag gttggagttt gattgcctgt atttgtagta gtttcgcagc
    12601 gaaatcagaa gctgctgaag cattgaggag catggcccaa atctataaaa tattcgtcaa
    12661 cgagatacct gtcacgatac tggaaggcaa cccatcagac ctgcctgaaa ccatagatga
    12721 acacaggccg gtgtattttt ttactgagcg tgataagata cgtaaggcat ttgcactggc
    12781 tgagaccgga aattatgtgc gaagtctaac catcattggt gaagatgcca ggcagatcag
    12841 acaaaccctg ttttcagcgt atcgcctgat tcgggcagcg ggaggggttg tcatgaacca
    12901 gcatcggcag atattgctga tccaccgcca cggcatgtgg gatctgccaa aaggcaaggc
    12961 agaagccgga gagcaaatgc agcgcactgc gctcagggag gtggaagaag aaacaggggt
    13021 caaaccgctg gtcatcgtgc agaaactgcg gccaacttac catacttatg tgacggttca
    13081 gaaggtcaga atacttaaga agaccaactg gtatctgatg cagcatgatg gaaaggggaa
    13141 gctcaggcct caggaagaag aaggtattga ttcagctctt tgggtttctg cagaagacct
    13201 tatacccaag atgcagggaa cctatggcaa catcagagat gtgctggacc aggccttgct
    13261 tactgtgtac ttttcgtaac aactaaagga accaggtgag catacggacc accgtgtcgc
    13321 accagttcgc gaacaatggt gctgctgatg tgggagaatt ggggagaagc tggcaggaat
    13381 atggtttccg gcccgttctc cagggaacga ttcaatgcgg cgatggtctt ttcatattca
    13441 aaatccatgg catttcgcac accccgaacg atccatccgg cctggtgtct ggcgcagaag
    13501 tcaacggtca ggccttcata ggcctctact gatatgttgt tgcgatgtcc ataaagatcc
    13561 tgcagccatt ggagtctttt ttccagggga aagaagcttt gtttttgtcc attcactccg
    13621 atggcaatga tcagcttatc aaacaggccg gcggcccggt ctatcagatc gtgatggcca
    13681 atggtgatgg gatcaaaaga ccccggaaat acggcaatcc tgctgttcat ggcatttcaa
    13741 atatactgaa tatggtggtg ccgtagtttc gtttcatctt caggttcggg tgttcgtcaa
    13801 agtgatggtt cggattatgt tcgagtatca gccaaccgcc tgaagccagc agtcctttct
    13861 ccatgataag gtcaggaatc ttatccaggt tgggcagtcc gtatggtggt ccagcaaata
    13921 tgaggctgta ggaagcagag gcagagcgaa tgtattcgaa tacatccatc tgcatcacct
    13981 gaaggtcaag cccgaattct tctgcctg
//
LOCUS       DAOMAX010000048        13978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_514_length_13978_cov_4.939381, whole genome shotgun sequence.
ACCESSION   DAOMAX010000048 DAOMAX010000000
VERSION     DAOMAX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_514_length_13978_cov_4.939381"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PK742_04260"
     CDS             <1..210
                     /locus_tag="PK742_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75835.1"
                     /translation="HDLLLHYLEEIMQTPVDVLTIAYEPAPGSRRASLSFHLTEKEKQ
                     DVRASVTGQENMQVYRQLGNLLSPF"
     gene            complement(196..1596)
                     /gene="rlmD"
                     /locus_tag="PK742_04265"
     CDS             complement(196..1596)
                     /gene="rlmD"
                     /locus_tag="PK742_04265"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQU75836.1"
                     /translation="MRKPRNRELNGLTVESVAAHGMGLARHEGKVIFIEKAIPGDVVD
                     VRLTKNKKDYAMAYPVRFEKRSALRSEAFCEHFGVCGGCTWQDVAYEQQLQFKHDLVA
                     DAFRRTGKVMELPAIPPVTGSLHDRYYRNKLEYTFSNNRWLTAEEIDNKEELNRNALG
                     FHIPGRFDKILDIRYCYLQPAPSNEIRLAVRAYAMLRNLTFYDLRTHEGYLRNLVIRN
                     TSTGGLMIILVVAEDRPETMAIIRHLQQAFPEITSLYYAINTKLNDAMYDLEMVHAGG
                     EKTIREEIDGISYHLGPKSFFQTNTHQAAILYRTALGMADLQADDVVYDLYTGLGSIA
                     LLAAGSCKKVVGVETIDAAVEDARKNAIANHIDNCTFVAGDMMKVFNDAFIEKHGKAD
                     VVITDPPRAGMHEDVCRTLLRLNPRSIIYVSCNPVTQARDVQWLSEGYRVTQIQPVDM
                     FPQTFHIENVVKLERR"
     gene            complement(1598..3457)
                     /locus_tag="PK742_04270"
     CDS             complement(1598..3457)
                     /locus_tag="PK742_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75837.1"
                     /translation="MRAFLFTTLAICATIPVTVQAQATRNTLPAAQPVVVSPSMVQHD
                     YFPVLFSTEAPAPGSGSYREFLMERKKELYDHYQPTGQRIATGDAAAPEVLTGFQGNP
                     YNFSVPNDNDVAISNDGMIVSVINSTINIFTETGEELYAVSLSAFSDTLDILESDFDP
                     RVKYDPIHDRFVLVFLNGFDPLTSYIIVAFSQTNDPTGLWNMYALPGNPKDNDRWTDY
                     PMIALTEGELFLTGNLIIPGEPWQTGFSETLIWQMSLDEGYSGADLNAIFWDDVFYGG
                     APIRNIHPVQGGSTPYGPDIYMLSNRNFSESNDSIFIVHVSGTLDDPGTTVTVELGLT
                     DLPYGVPPVARQYNNHEFDTNDGRILGSFMENGQIQFVSNTLDPATGFCGIYHGIITD
                     LDGDYSIHGYIIGDDTMDLGYPNVSYTGRYDNDNQSIITFDHTAPEVFAGMSGIFYND
                     GNYSELVNIHTGDSYVNILSGYYERWGDYTGSQRKYNEPGVIWVSGNFGREVDLGPFT
                     NRENATWVASLRTNDSLPLALESDREPVFTNVYPNPVQTWFQLDLELTQNAVLGFSVL
                     DVNGKLVDELLYGEGKTGNNTFRFSTASLPAGIYFLQITANGSLWRTERIVKQ"
     gene            complement(3480..4628)
                     /locus_tag="PK742_04275"
     CDS             complement(3480..4628)
                     /locus_tag="PK742_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQU75838.1"
                     /translation="MLIAVNARFLIRDKLEGIGWYTYETIKRLVQRHPEHRFLLLFDR
                     EPDASFLFADNVEPLQVGPPARHPWLWQWWFQRSLPAVLKKYKPDVLLSPDGFLPLKV
                     DIPTVLVIHDLGFEHYPEHTPPMVNRFYRRNTPKYAKAASRIVTVSEFSRQDIIKRYH
                     IDPEKVDTVYNGANELYQPIPAMEQDQVRQQYTTGAPYFLYVGSIHPRKNVEVLLRAF
                     EALKTQYQPHVKLVLAGRMAWKTDKTKKVMEQLSCRNDVVFTGHLQQEELARITASAA
                     ALVFPSLFEGFGIPVVEARYSGIPVICSNSSSLPEVAGPDALYFDPEDVAGLTDAMMT
                     FLEDQDTYAEAFSDTLTVRTRFNWDRSADGLEEAIHKILPDFIPVSGT"
     gene            4760..6217
                     /locus_tag="PK742_04280"
     CDS             4760..6217
                     /locus_tag="PK742_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQU75839.1"
                     /translation="MKRSFLSNLAFLLFLNFLVKPFWILGIDRTVQNVTGPDQYGLYF
                     SLFNFSLLFQVLLDFGINNFNNREIAKYPEKLGQTWSTMLQSKAIFALLYAALVLIVA
                     VLIGFDQTHWRILGWMILMQVMLSFFTYLRSNVSALHLFRHDAVLSVSDKLFAGTLCA
                     LLFWTKVLPWEITVKNFIICQILGYSLASVLAILMLLGRRIHWKWTTDLQLIRQVIRK
                     SAPFALLTFLMAAYYRIDGVMIERMLGAEGAHQAGVYASAFRLLDALSIIGYMFAAIL
                     LPMFSRMQEEGSNVQPLLETGLRLMACISVPAGTTFIFFRQEIMQALYADATNQYGQI
                     FGWLMAGFVLISLVYILGTMLTALGSLRILNRIAAAGLVVNVALNLLLIPTQQAVGAA
                     IATVCTQTIVLILHLAAVYSRTDITFHQGMWLRLAGLTAACVVICPGVLWLNLPWAIA
                     MGLCLLLGACLALLLGLVKRHDWKPLLHEAAEPAT"
     gene            6259..7152
                     /locus_tag="PK742_04285"
     CDS             6259..7152
                     /locus_tag="PK742_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HQU75840.1"
                     /translation="MTRKHNDLVELVRILLKWRKPIIIVTVIAAIGTAAFSWFFMPDY
                     FKSSVNFYPSNPIMTDRQVLYSTTVGEIEIDYFGGTADVDRILTLAHTSRMKDYIINK
                     YHLTEHYGYDSTKTLARYKTKKKFEKNYKAVETEFGAVEITVWDKDKQLASDMANHIA
                     LTIDNQNKDMITRDKNVVIQTFKQDIAKKEAANDSLANVIEQAKASGQRAERIMQLET
                     QFETSLTDLNNSRKLLEQYQTSASNNFSTIHVTEEAFPAIRKDKPVRSLIVIGATIGA
                     FLFMLILAVVTENYRKIREEL"
     gene            7154..8587
                     /locus_tag="PK742_04290"
     CDS             7154..8587
                     /locus_tag="PK742_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQU75841.1"
                     /translation="MDLLRRISEKQLLFTGAGILLISIFAAILANEPLICLAPVAFLI
                     TCQIIIDFRPLFFLLLLSTPLAIEYRFDGGFGTDLPTEPLEVLLMFTFIFYALLRKEQ
                     ISRRFLLHPVSILLYIHLAWIIIAVIFSQDVAISFKWLLAKIWYVVVFYVIGGHIMQD
                     KNAWRTFFWCLFVPTVGVVIHTLMRHAAYNFAFSEVNKSMWPIFRNHVNYAVYLALFL
                     PFTFVAAGWYERGSWKKFFIRLGGILIAVAIYFSYTRSAWLSLAVALVCYFLIRWNTL
                     MPAVFLGIAGAIIFVVYMLHDNTYLQYAPDYKKTIYHTDFNQHMEATTSLEDVSSAER
                     IYRWIAGVKMIEDKPFIGFGPAQFESNYQEYAVNKFTTYISRNEEKSTIHNYYLMVTV
                     EQGFPGLLFWMILLVAILYYGQRSYTRSGPAHKPIMMATLLAFITILINIALSDLIEA
                     DKIGTMFFLFAAMIVVADLKERKGELD"
     gene            complement(8584..9834)
                     /locus_tag="PK742_04295"
     CDS             complement(8584..9834)
                     /locus_tag="PK742_04295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQU75842.1"
                     /translation="MAKAGTYMIDTLSEQLKLKQLELDTLLNITNAINNNASASELFE
                     LFSHTLVNQLGVERMLLVRYENEWAIQAKAGYSNGLKPDVETDLLPIRQLTFTEELAD
                     PKPAILSAFDIVIPVYHKDRPLAYLLISNPQVANLEKVEDKIKFAQTVTNIILVAIEN
                     KRLFNREKEQEVLQRELELAAQVQSMLIPDRLPSNDFVDVDAIYMPHTNVGGDYYDFL
                     YLNDEEFFFCIADISGKGMSAALLMANFQGQLRELVKKNHPTIDEFLQELNSGVLHNT
                     RGEKFITMFLGKYNTRTRVLKYVNAGHNPPVLINSKGNRLLDLGCTILGIFDELPQVR
                     MGQIPIVEDTTIMCYTDGLTDQVDMNGKPFSTEDITSLLEENLGEDVSAINHVLGNRI
                     MQYRNGQSFTDDITIMTVRIFGRN"
     gene            complement(9861..11237)
                     /locus_tag="PK742_04300"
     CDS             complement(9861..11237)
                     /locus_tag="PK742_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU75843.1"
                     /translation="MERQKVLIQLLREILVAIAVACIIYVLVVFVSGQQFSVSAGISF
                     VLRACCCWWILFRVPAVLLRRWWKKRSAVREVSRVATRFLMNRNALFGALVLLYTSII
                     FTGAHLLAPDGSSHAAQQVNELAFCPPGTEAVLFSLPHVKHTHRESFLKVLLRGRDLQ
                     ERLIAVTDARADTSGVYVTRFMGDGIIGPEMSYRWEELGLLEPEDDAAAESKLKELLI
                     HRTYHLGTDDLGRDYLSRLIVGVRVSFAVGFVAVFIALLIGIPVGAVAGYYQQHPPVI
                     HLGKRRWKLPADGFIMWTMSVVWAIPTLLLVFPVVYAFGQQFYTIFIAVGITMWVDIA
                     RVVRGQVMQVRELEFIEATRSFGYGKMRTILLHILPNITGPVIVITAANFAYAILTES
                     GLSFLGIGVQPPAPSWGLMISKYKDNLITDPYLALLPGAAITLLVLAFFLVGNGLRDA
                     MDIRMQES"
     gene            complement(11216..12352)
                     /locus_tag="PK742_04305"
     CDS             complement(11216..12352)
                     /locus_tag="PK742_04305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU75844.1"
                     /translation="MIWYSLLVYGMTILYALFIIYYLWGWRRLKTFQPVNTIPEHKVT
                     IIIPARNEARHLPDLLQDLADQQYPNQLLEVIVVDDFSTDHTAQIAAQFNGLPVRVIS
                     LAKHLEPEAPKPAHKKRAIELAVEQATGTLILTTDADCRIGPGWVAAMESYHRETGKV
                     FIAGPVTYFPDTTLLGKFQTMDFMTLVGIAAASIRNGFYNLCNGANLGYTREAFLAVD
                     GYDQADHTPSGDDMMLMHKMGKKFPGQVGFIRNQQAVVSTYPAPDFRTFWQQRLRWSS
                     KSTNYEDKRITIILLFAYLFNLLLVVHIIAGAFYPVFLRLAMWQFIVKIGFDTLFTYA
                     IAHFFRRENLLWSMLPVQVLHIFYIIVIGPASLFGSFEWKGRKF"
     gene            complement(12349..13158)
                     /locus_tag="PK742_04310"
     CDS             complement(12349..13158)
                     /locus_tag="PK742_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQU75845.1"
                     /translation="MADQLQLTYKKSTILFHRFGHGPEAMLAFHGFSETGSSFVCLEP
                     SLGQRYTVWAIDLPYHGGTRWRESTHFAKADLEAIVQQLMEENGLHTISLFGFSMGGK
                     CAMEICHRFPGQVRELYLLASDGIKTRTVYNVAVYPAWGRSLFKSIISRPGWFFTFIR
                     IMHRMKILSPWLYRFTMNHMDTEEKRQRLYDTWVSMANFEIDIKSLKQTIRQHAIPLK
                     LVFGERDEVIPPAVAHEMAAGLPTATVRIIRRGHYFIDDKLNHTLEELTEI"
     gene            13296..>13978
                     /locus_tag="PK742_04315"
     CDS             13296..>13978
                     /locus_tag="PK742_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HQU75846.1"
                     /translation="MPRDTSIQHILIIGSGPIIIGQACEFDYSGSQASRSLREEGIRV
                     SLINSNPATIMTDPVTADHIYLLPLEPASIEKILKENIESGDRIDAVLPTMGGQTALN
                     LAKEVNEMGIWEKYGVRLIGVDIEAIDRAENRELFRQLMVELGISVAPSHIANSFLEG
                     KEYAQQIGFPLVIRPSFTLGGTGGSFVHSKDDLDAALQRGLTASPTHEVLVEKAVLGW
                     KEYELELLR"
BASE COUNT         3470 a         3526 c         3405 g         3577 t
ORIGIN      
        1 catgatcttt tactgcacta tctggaagag atcatgcaga cacccgttga tgtactgacc
       61 attgcatatg agccagcacc gggcagcagg cgggcttcac tcagttttca cctgacagaa
      121 aaagaaaagc aggatgttcg ggcttctgta accggacagg agaacatgca ggtgtacagg
      181 cagcttggca acctgttatc gccgttctag tttcaccaca ttctcgatgt gaaatgtttg
      241 cgggaacatg tcaaccggtt gtatctgcgt aacccggtaa ccctcactca gccattgtac
      301 atcgcgggcc tgtgtaacag gattacagct cacatagatg atgctgcgtg ggtttagtcg
      361 cagtaaggtc ctgcacacat cttcatgcat acccgccctg ggtggatcag tgatgaccac
      421 atcagccttg ccgtgttttt caataaaggc atcattgaac accttcatca tatcgccggc
      481 tacgaaagtg cagttgtcaa tatggttggc gatcgcattt tttctggcat cttccaccgc
      541 tgcatcgatg gtttccactc ccaccacttt cttacaggac ccggctgcca gtagtgcgat
      601 acttcccagt ccggtataga ggtcataaac cacatcatca gcctgcaggt ctgccatgcc
      661 cagagccgtc cggtacagga tagctgcctg atgtgtgttg gtctgaaaaa atgatttggg
      721 gccgagatga tagctgattc cgtctatttc ttcgcgaatg gttttttcac caccggcatg
      781 caccatctcc agatcgtaca tagcgtcatt cagcttggtg ttgatggcat aatagagcga
      841 agtgatctcc gggaaggcct gctgcagatg cctgatgatg gccatggttt ccggccggtc
      901 ttcggctacc accagaatga tcataagtcc tcctgtggag gtgtttctga tgaccaggtt
      961 gcgcaggtat ccttcatggg ttcgcagatc atagaaagtg aggttacgca gcatggcata
     1021 agcccgcact gccaggcgta tctcgttgga aggagctggc tgcagatagc aatatctgat
     1081 atccaggatc ttgtcaaatc gtcccggaat atgaaaacca agggcgttcc tgttcagctc
     1141 ttccttattg tcgatctcct cggccgtaag ccaacgattg ttggaaaaag tatattcaag
     1201 tttattgcga taataccggt catgcagcga gccggtaacc ggtggaatag caggcagctc
     1261 catgacctta ccggttctgc gaaaggcatc ggccaccagg tcatgtttga actgcagctg
     1321 ctgttcatag gccacatcct gccaggtaca accgccacaa acaccaaagt gctcgcagaa
     1381 agcttctgag cgaagcgcac tgcgcttctc aaagcgcacc ggataggcca tggcatagtc
     1441 tttcttgttt ttggtaagac gtacatccac tacatcgccg gggatggcct tctctataaa
     1501 aatgaccttc ccttcatgcc gggcaagacc cataccatgg gctgccacgg attctacggt
     1561 caggccgttc aattcgcggt tgcggggttt tctcatctta ttgcttcacg atcctttcag
     1621 tgcgccagag tgatccgttt gcagtgatct gcaggaaata gataccagcg ggcaaagatg
     1681 cagtagaaaa gcgaaacgtg ttgtttcctg tcttgccttc gccatacaga agttcatcca
     1741 ccagtttacc attcacgtca agaacggaaa atcccagcac ggcattctgt gtcagctcaa
     1801 gatccagctg gaaccaggtt tggaccgggt taggatagac attcgtgaat accggttcac
     1861 gatcactttc caatgcgagg ggcaagctgt cattggttct caggctggca acccaggtgg
     1921 cattttcacg gttggtgaaa gggcccagat ctacttctcg tccaaaattg cctgacaccc
     1981 agatcacacc cggctcattg tatttgcgct ggcttccggt ataatcgccc cagcgttcat
     2041 aataaccgct caggatgttc acatatgaat cgccggtatg aatattcacc agttctgaat
     2101 agttgccatc attataaaag atgccagaca tgccagcgaa cacttcgggt gcagtatgat
     2161 caaaggtgat gatgctctgg ttgtcgttgt catagcggcc ggtataggat acattcggat
     2221 agcccagatc catggtgtca tcgccaatga tgtatccgtg tatagaatag tctccatcca
     2281 gatctgtgat gatgccgtgg tagataccgc agaagccggt tgccggatcc agtgtattgg
     2341 acacgaactg tatttgtccg ttctccatga agctgcccag gatgcgcccg tcgttggtat
     2401 caaactcatg gttgttatac tgacgggcca cagggggtac cccatatggc aggtcggtaa
     2461 ggccgagttc taccgtgacc gttgtaccgg gatcgtccag tgttccgctt acatgtacga
     2521 tgaaaataga atcattgctt tcagagaaat tcctgttaga caacatgtag atatccgggc
     2581 cataaggcgt gctgccccct tgtacagggt ggatgtttcg aatgggagca cccccgtaga
     2641 atacatcatc ccaaaaaatg gcattcagat cggcgccact gtatccttcg tccagactca
     2701 tctgccagat gagtgtttcg ctgaaaccgg tttgccaggg ttcaccggga atgatgaggt
     2761 ttccggtcag gaacagttca ccttcggtca gtgcgatcat ggggtagtct gtccaccggt
     2821 cgttgtcttt cgggttgccg ggtaaggcat acatgttcca gagtccggtg ggatcgtttg
     2881 tctgtgaaaa ggccacgatg atataactcg tgagcggatc gaagccattc agaaacacca
     2941 gtacgaaacg atcatgaatg gggtcatact tcacccttgg gtcaaagtct gactcgagta
     3001 tatccagcgt atcactgaat gcgctgagcg atacggcata cagttcttca ccggtttctg
     3061 tgaatatatt tatggtgctg ttgatgaccg atacgatcat gccatcgttc gaaatggcta
     3121 cgtcgttgtc gttcggaaca gaaaaattgt aggggttacc ctgaaaccct gtcagcactt
     3181 ccggagcagc tgcatcgcct gttgctatac gttgtcctgt tggctgatag tgatcataca
     3241 actctttctt gcgttccatg agaaattctc tgtaggagcc ggagccggga gccggagctt
     3301 ctgtactgaa gagtacaggg aaataatcat gttgtaccat tgatggcgag accacaacag
     3361 gttgtgcggc aggcaatgta ttccgggtgg cctgtgcttg aacggttaca ggaatggtgg
     3421 cgcaaatagc cagtgtggta aacagaaacg ctctcatcag gtaaaaatac gacaatcact
     3481 caagtgcctg ataccggtat aaagtccggc agtattttat gtatggcctc ctccagacca
     3541 tctgcagaac gatcccagtt gaaacgggtc cttacggtca gcgtatcact gaatgcttcc
     3601 gcataagtat cctgatcttc caaaaaggtc atcatggcat cggtcagacc ggccacatct
     3661 tccggatcga aatataatgc gtcaggacct gccacctcgg gcaaactgga actgttggaa
     3721 cagatgacag gaataccgct gtatctcgct tccaccaccg gaattccaaa accttcgaag
     3781 agcgaaggaa acaccagtgc cgccgcagat gcagtgatgc gggccagttc ttcttgctgc
     3841 agatgcccgg tgaagaccac atcatttctg caggaaagct gctccatgac ctttttggtc
     3901 ttatcggtct tccatgccat tcgcccggcc agtaccagtt tgacatgtgg ctgatactgt
     3961 gttttcaaag cttcgaatgc acgcaacaac acctccacat tttttctcgg atggatggaa
     4021 cccacataca gaaaataggg tgccccagtt gtgtattgtt gtctgacctg atcctgttcc
     4081 attgcaggaa ttggctggta taattcattc gcaccattgt acactgtatc tactttctcc
     4141 ggatcgatgt ggtacctttt aatgatatcc tgcctggaaa actctgacac tgttacaatg
     4201 cgtgaagcag ctttcgcata tttaggtgta ttgcgccgat agaaccggtt gaccatgggc
     4261 ggcgtgtgtt cggggtaatg ctcaaaaccc agatcatgaa tgacaagaac cgtaggtata
     4321 tccaccttca aaggcaggaa tccgtcgggc gagaggagca catcgggctt atatttcttc
     4381 agtaccgcag gaagggaacg ctggaaccac cattgccaca accaggggtg ccttgccgga
     4441 ggaccaacct gcagcggctc cacattgtca gcgaataaaa atgatgcgtc cggctcccgg
     4501 tcgaacaaca acaaaaaccg atgctccggg tgccgctgca ccaggcgctt gatggtctca
     4561 taggtgtacc aaccaatacc ttccagttta tccctgataa ggaaacgtgc attgacagca
     4621 atcagcatga gatcaaatag gtggttttcc ggtaaacaga acaggtagag ttggttcgct
     4681 aagcagagag attggtaagc gttaccttat aaaaagtggt ttgatgcaat aatactattt
     4741 ttatcgcata atctgttgca tgaagcggag cttcctgtct aaccttgcat tcctcttatt
     4801 tctgaatttt ctggtcaagc ctttctggat actgggcata gacaggactg tgcagaacgt
     4861 gaccggacct gatcaatacg ggttgtattt ctctctgttc aacttcagtt tattattcca
     4921 ggtattactt gattttggca tcaacaactt caacaaccgg gagatcgcaa aatatccgga
     4981 aaagctgggg cagacctgga gtaccatgtt gcagtcaaaa gcaatattcg ctttgttgta
     5041 tgctgcgctg gttttgatag ttgccgtgtt gatcggattt gaccagacac actggcgtat
     5101 tctgggatgg atgatcctca tgcaggtcat gctgtctttc tttacttacc tgcgcagcaa
     5161 tgtgtcagca ttgcatttgt tccggcatga cgctgtgtta tccgtcagcg acaaactgtt
     5221 tgcgggcacg ttgtgtgctt tgttgttttg gacgaaggtc ctgccctggg aaataacggt
     5281 gaaaaacttc atcatctgtc agatactggg ttattcactg gcctctgtgc tggccatcct
     5341 gatgctgctg ggcaggagga tccattggaa atggaccaca gaccttcagc tgatcaggca
     5401 ggttatcaga aagagtgcgc cgtttgcctt gctcactttt ctcatggcgg cctactaccg
     5461 catagacggg gtcatgatag aacgtatgct tggcgcagaa ggcgcccacc aggcaggtgt
     5521 gtatgcctct gcatttcggt tactggatgc gctgagcatc atcggctata tgttcgccgc
     5581 tatcttactt cccatgttca gcaggatgca ggaagaaggc agcaacgtgc agccgctcct
     5641 ggaaacaggc ctgcggctta tggcctgcat cagtgttcct gccggtacca cgttcatctt
     5701 cttcaggcag gagatcatgc aggcattgta tgcggatgct acgaaccagt acgggcagat
     5761 atttggctgg ctgatggccg gtttcgtcct gatcagtctg gtgtatattc ttggcaccat
     5821 gcttacagcg ctcggatcgc tgcgcatact gaaccgcatc gcagcggctg gcctggtggt
     5881 gaacgtggca ctcaatctcc tgctcatacc cacacagcag gcagttggtg ctgccattgc
     5941 cacggtttgc acacaaacga tcgtactgat tctgcatctg gcagcagttt atagcaggac
     6001 agatataact ttccatcagg gtatgtggtt gcgtctggcc gggttaacgg cagcctgtgt
     6061 ggtgatctgt cccggtgttt tatggctgaa cctgccctgg gccatagcga tggggctttg
     6121 tctgttgctg ggtgcctgcc tggcgctgct gcttggcctg gtaaagcgtc acgactggaa
     6181 acctttgctg cacgaagctg cagagcccgc cacttaggta gtttttctac ctttgcacga
     6241 ctaaaaaaaa tcatacggat gactcgtaaa cacaatgatc tggtagaact ggttcgcatt
     6301 ctgctgaaat ggcgtaagcc tatcatcatc gtgaccgtga tagctgctat cggtaccgca
     6361 gctttcagtt ggttcttcat gcctgattac ttcaagagtt ccgtcaactt ctacccttcc
     6421 aacccgatca tgaccgaccg tcaggtgttg tacagcacca ccgtgggcga gatcgagatc
     6481 gactatttcg gtggtactgc agatgtggac cgcatcctga cccttgcgca taccagcagg
     6541 atgaaagatt atatcatcaa taagtatcac ctgactgaac actacggata tgactctacc
     6601 aagaccctgg ccaggtataa gaccaaaaag aagtttgaaa agaactacaa agctgtggag
     6661 accgaattcg gtgcggtaga gatcactgtt tgggataagg acaagcagct ggccagcgat
     6721 atggccaacc acattgcgct gaccattgac aatcagaaca aggacatgat cacccgcgac
     6781 aagaatgtgg tgatccagac tttcaagcag gatattgcca aaaaagaagc agccaatgat
     6841 tctctggcca atgtgatcga acaggccaaa gcctccggac agcgggcgga gcgcatcatg
     6901 cagctggaaa cccagtttga gacctctctg acagatctga ataattcacg caagctgctg
     6961 gaacaatacc agacatcagc cagtaataac ttcagcacca tacacgtaac ggaagaagct
     7021 tttcctgcca ttcgcaaaga caaaccggtg cgttcgctga tcgttatcgg cgctaccatc
     7081 ggagccttcc tgttcatgct gatcctggct gtggtcacag aaaattatcg caagatccgc
     7141 gaagaactct gagatggatc tgctcaggcg catcagtgaa aaacagttgt tgttcacagg
     7201 agccggtatc ctgctgatca gcatctttgc tgccatactg gccaatgagc ctttgatatg
     7261 tctggcacct gttgcctttc tgataacctg ccagatcatc atagatttca gaccgctttt
     7321 ctttctgtta ctgctctcta cacctttggc cattgaatac cgttttgacg gtggttttgg
     7381 cacagacctg ccaacagaac cactggaggt gctgctcatg ttcaccttta tcttttatgc
     7441 cctgttgcga aaggaacaga tcagcaggcg cttcctgttg caccctgttt ccatcctgtt
     7501 gtatatacac ctggcatgga tcatcatcgc ggtgatcttc tctcaggatg tagccatctc
     7561 tttcaaatgg ctgctggcca agatctggta tgtggtggtg ttttatgtga taggcgggca
     7621 tatcatgcag gataaaaatg cctggcgaac atttttctgg tgcctgttcg tacctacagt
     7681 aggtgtggtc attcatacat tgatgcgaca tgccgcctac aattttgcct tcagcgaagt
     7741 aaacaaaagc atgtggccta ttttcaggaa ccatgtgaac tatgccgttt atctcgccct
     7801 cttccttcct ttcactttcg tggcagcagg ttggtatgaa aggggaagct ggaaaaagtt
     7861 ctttatccgg ctgggaggta tcctgatagc agtggccatc tatttttcct atacccgttc
     7921 agcctggcta tcgcttgcag tagctctggt atgctatttt ctcatacgat ggaacaccct
     7981 tatgccagca gtgtttctgg gcatcgccgg tgctatcatc tttgtggtgt atatgctgca
     8041 tgacaatacc tatctgcagt atgcacctga ctacaagaag accatctacc atacggactt
     8101 caaccagcac atggaggcca ccacctcgct ggaagatgtg tcaagtgcag aacgtatcta
     8161 tcgatggata gccggtgtca agatgataga agacaagccc ttcatcgggt ttgggcctgc
     8221 ccagttcgaa tccaattacc aggagtatgc ggtcaataag ttcactacct atatcagccg
     8281 gaacgaagaa aaaagcacca tccacaatta ctacctgatg gtaaccgtag agcagggctt
     8341 tcccggtctg ctgttctgga tgatcctgct ggtggccatt ctgtattacg gacaaagaag
     8401 ctacacccgg tcaggtccag cacataaacc catcatgatg gccacgctac tggctttcat
     8461 cactattctg atcaatattg cactcagcga tctgatagag gctgataaga tcggcacaat
     8521 gttcttccta tttgcagcca tgatcgtggt tgccgatctg aaagaacgca aaggcgaact
     8581 ggattaattc ctgccgaaga tcctgacggt catgatcgtg atgtcatctg taaatgactg
     8641 accatttctg tattgcatga tgcggtttcc cagcacatgg ttgatggcac ttacatcttc
     8701 ccctaaattc tcttccagca gagaggtgat gtcttcggta gagaaaggct tcccgttcat
     8761 gtcaacctga tcggtcaggc catctgtgta acacatgatc gtagtgtctt ctacaatggg
     8821 gatctgtccc atgcgcacct gaggcagctc atcaaaaata ccgaggatgg tacaacccag
     8881 atccagtaaa cggttgcctt ttgagttgat gagtaccgga ggattatgac ctgcgttgac
     8941 gtacttcagc accctcgttc tggtattgta tttacccagg aacatcgtga tgaatttctc
     9001 ccctcttgta ttatgcaaca ctccgctgtt cagttcctgc aaaaattcat caatggtggg
     9061 atggttcttc ttcaccagtt cccgaagctg cccctggaaa ttggccatca gcagggccgc
     9121 actcatcccc ttcccgctga tgtctgctat acagaaaaag aattcctcat cgttcagata
     9181 gagaaagtcg tagtaatcgc cgcccacatt ggtgtgtggc atatagatcg catccacatc
     9241 tacaaaatca ttggagggaa gcctgtccgg aatgagcatg ctctggacct gtgcggccag
     9301 ttcgagttcc ctttgcaata cttcctgttc tttctcgcgg ttgaacagtc ttttattctc
     9361 gatcgctacg agtatgatgt tggttaccgt ctgggcaaat ttgatcttgt cttctacttt
     9421 ctccagatta gccacctgtg ggttgctgat cagcagatac gccagcggac gatccttatg
     9481 ataaaccgga atgacaatat caaaagcaga caagatcgca ggcttgggat ctgccagttc
     9541 ttctgtaaaa gttagttgtc tgatgggcag caggtcggtt tccacatcgg gtttcaggcc
     9601 attggaataa cccgcttttg cctgaatggc ccattcattc tcatacctga ccagcaacat
     9661 acgttctaca cccagctgat tgaccagtgt atgactgaac aattcgaaca actctgatgc
     9721 agatgcattg ttgttgatcg cattggtgat gttcagcaag gtgtccagct caagctgttt
     9781 cagtttgagt tgttcactca gggtatcgat catataggta cctgcctttg ccatcagcct
     9841 gcaagatacg gagaagcgga ttagctctcc tgcatgcgaa tgtccatcgc atcacgcagg
     9901 ccattgccta ccagaaaaaa agcaagcacc agtaaggtga tggctgctcc cggaagtagt
     9961 gcgagatacg gatctgtgat aagattatcc ttgtatttac tgatcatcag cccccaggat
    10021 ggggcaggtg gctgtacacc tataccaagg aaactcagac cggattcggt caggatggca
    10081 taggcaaaat tagctgcagt aatgacgatg accggtccgg taatgtttgg cagaatatgc
    10141 agcagaatgg tgcgcatttt accataacca aaactgcggg tcgcttcaat gaattcaagc
    10201 tctcttacct gcatgacctg tcccctcacc acacgggcta tgtccaccca catggtgatg
    10261 cctactgcaa taaaaatcgt atagaactgc tggccgaagg catagactac aggaaatacc
    10321 agcagcaatg tcggaatggc ccagaccaca ctcatcgtcc acatgataaa accatcggct
    10381 ggcagtttcc atcttctttt tcccagatgt atgaccggtg gatgctgctg ataatatcct
    10441 gctacagccc ctaccggaat tcctatcagc agcgcaatga agaccgcaac aaatcctaca
    10501 gcaaatgata cccgcacacc tactatcaac cggctgagat aatcacgacc cagatcatct
    10561 gtacccagat gataggtcct gtggatcaga agttctttca gttttgattc agctgccgca
    10621 tcatcttccg gctccaataa ccctaactcc tcccagcgat aagacatttc cggtccgatg
    10681 atgccatcgc ccatgaatct ggtaacatac accccactgg tatctgccct ggcatcggtc
    10741 acagcaatca gtctttcctg caagtctctt ccacgtaaaa ggaccttcag aaaggattcc
    10801 cggtgtgtgt gtttgacatg cggaagactg aacaagacag cctctgttcc aggtggacaa
    10861 aatgccagtt cattgacctg ttgcgccgca tgcgaactac catccggagc aagcaaatgt
    10921 gcccctgtga aaatgatgga tgtatatagg agcacaagtg caccaaaaag tgcattccgg
    10981 ttcatcagaa atcttgtggc aaccctggat acctcacgaa ctgctgagcg ctttttccac
    11041 cacctgcgaa gtaagactgc aggcacacga aacaagatcc accagcaaca acatgcccgg
    11101 agaacaaagg aaatgccggc agaaacagag aattgctgcc cggatacaaa taccactaat
    11161 acatatatta tgcacgcaac tgcgatggcc accaggatct ccctgagcag ttgtatcaga
    11221 actttctgcc tttccattca aaactgccaa acaaggaggc aggacctatc acgatgatat
    11281 agaaaatatg cagcacctgt accggcagca tgctccacaa taaattctct cttcgaaaga
    11341 agtgtgctat ggcgtaggtg aacaaggtat cgaacccgat cttgacaatg aactgccaca
    11401 tggccagacg aaggaagacc ggataaaatg cgccggctat gatatgcacc accaacagca
    11461 gattgaacag gtaagcgaag agcaggatga tggtgatgcg tttgtcctca tagttggtgg
    11521 atttactgga ccagcgtaag cgctgttgcc agaaggtcct gaaatcaggt gcaggatagg
    11581 tggacacaac cgcctgctga ttgcgaatga aaccgacctg tcccggaaat tttttgccca
    11641 tcttatgcat gagcatcatg tcatctccac tgggagtatg atcagcctgg tcatagccat
    11701 caacagcaag aaacgcttca cgggtatagc ccaggttggc gccgttacag agattgtaaa
    11761 acccattccg gatggacgct gcggcaattc ccacaagggt catgaaatcc atggtctgaa
    11821 acttacccag caatgtggta tccggaaaat aagtgaccgg accggctata aagacctttc
    11881 ctgtttcccg atgataagat tccatcgctg ccacccaacc cggaccaatg cgacaatcag
    11941 catcggtggt caggatcagt gtgcctgtgg cttgttctac cgcaagttct attgcccgct
    12001 ttttatgagc aggctttggc gcctctggct caaggtgttt tgccagtgaa atgaccctga
    12061 ccggaaggcc attgaactgc gctgcgatct gtgccgtatg atctgtagag aaatcatcta
    12121 ccactatgac ctccagcagt tgattaggat actgttggtc tgcaagatcc tgcaacagat
    12181 cgggcaggtg tctggcttcg ttgcgtgcgg ggatgatgat cgtaacctta tgctccggaa
    12241 ttgtattcac cggctggaat gtcttaagtc ttctccatcc ccacagataa tagataatga
    12301 acagggcgta taatatggtc atgccatata ccagcaaact ataccatatc atatctcagt
    12361 tagttcttcc agtgtgtgat tgagcttgtc gtctatgaag tagtgtccgc ggcggatgat
    12421 acgaacagta gcagttggta aacccgcagc catctcatgc gccacagcag gtggaatgac
    12481 ctcatcccgt tcgccgaaga ccagcttcag cggaatggcg tgttgccgga tcgtctgttt
    12541 caaacttttt atatcgatct caaaattggc catggacacc caggtatcat acaatcgctg
    12601 tcttttttcc tctgtgtcca tgtggttcat ggtgaaccgg tacaaccagg gcgataatat
    12661 cttcatacga tgcatgatcc ggataaaggt aaagaaccag cccggccgac ttattatact
    12721 tttaaacagc gaacgccccc aggccggata gactgccaca ttatagaccg ttctggtctt
    12781 gatgccatcg ctggccagca gatacaactc cctgacctgc cccggaaacc gatggcagat
    12841 ctccatagca cattttcctc ccatactgaa gccaaaaaga gaaatggtgt gcaggccatt
    12901 ttcctccatc agctgctgta caatggcttc cagatcggcc tttgcaaaat gcgttgattc
    12961 ccgccacctg gtgccgccat ggtagggcag gtcaatggcc cagaccgtat agcgctgacc
    13021 cagagacggt tccagacaaa cgaaactgct gcctgtttca ctgaatccat gaaaagccag
    13081 catggcttcc ggtccgtgtc cgaaacgatg gaacaggatt gttgatttct tgtatgtaag
    13141 ctggagttga tctgccatgc acgttggact tgcgtgattt ttggcgtact tttgtgctcc
    13201 ggaatgcagt tcaaggcaaa ggtagaaatc ttcttcactc cggctctcct gcctttcatt
    13261 tcccccattt tttccgatcg taaaagaact tttatatgcc cagagatacg tctatccagc
    13321 acattctgat cattggcagc ggaccgatca tcatcggcca ggcctgtgaa tttgattact
    13381 ccggttccca ggcttccaga agtctgcggg aagaaggcat ccgcgtctct cttatcaatt
    13441 ccaatccggc caccatcatg acagatccgg tcacggcaga tcatatttat ctgctgcccc
    13501 tggaaccggc ttccattgaa aagatcctga aagaaaatat agaaagcggc gatcgtattg
    13561 atgccgtgct gcctaccatg ggcggacaaa ccgcactcaa cctggccaaa gaggtcaatg
    13621 agatgggcat ctgggaaaaa tacggcgtgc gactgatcgg tgtcgatatc gaagccatag
    13681 accgggccga gaatcgtgaa ctcttccggc aactgatggt ggaactcggc atctctgtgg
    13741 caccttccca tatcgccaac tccttcctgg aaggaaagga atacgcccag cagatcggtt
    13801 ttccgctggt catcagacca tccttcaccc tgggtggaac cggcggaagc ttcgtacatt
    13861 ccaaagatga tctggatgca gctttgcaac gcggactgac cgcttcgccc acacatgagg
    13921 tgctggtgga aaaagcggtg ctgggatgga aagaatacga actggaattg cttcgcga
//
LOCUS       DAOMAX010000049        13972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_516_length_13972_cov_4.987569, whole genome shotgun sequence.
ACCESSION   DAOMAX010000049 DAOMAX010000000
VERSION     DAOMAX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13972
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_516_length_13972_cov_4.987569"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1503
                     /locus_tag="PK742_04320"
     CDS             157..1503
                     /locus_tag="PK742_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQU75847.1"
                     /translation="MKRISHHSRLSWLLASLLFLPVLVSGQAIPVTVGMNSEGQWQLY
                     RNGEPYYINGVGGTDHLDLAAACGANSFRTWSPDDAGELLDEAQRLGMTVMLGLWVQH
                     ERHGFDYDNESKVKAQLDYFTEVVKTYKDHPALLLWGVGNEVDLFYSNTKVWNAIEDI
                     AAMIHQIDPNHPTSTVTAGLDPAEVRLIMANAPSIDIYGINTYGDIGIVPDTIRSAGW
                     QGPYIISEWGPNGHWEVSKTKWNAPVEQSSSEKAASYKARYNNYIAGDRVKCVGSYVF
                     LWGQKQETTSTWYGLLSEDGRQSEAIDELIRAWTGKEPANRAPAIASASLNGQSKGAD
                     IILQPGQPCQALATVTDADGDQMKFIWEVVSESQDIKAGGDAESRPPSVKGLGMRKDS
                     QQVSFRAPQSEGAYRLFLYVYDAANHYAYLNIPFYVLPDPSGEQYQKAIQLREQSL"
     gene            1575..3554
                     /locus_tag="PK742_04325"
     CDS             1575..3554
                     /locus_tag="PK742_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75848.1"
                     /translation="MSGTRTSNLKMFVSGSFIMWLILICMMAANTAVAQVEDSTETAL
                     GIRPAEEPSPLRKISVSGFYRFFATYTTHDLPYVLNPVINDTVLPASLFIGDDSQLPN
                     LQLTISGKPAPHTSFSFDVFAFQFLNGAIGTTYGAQVPDSLRPSFQDPLSGTRLGGQM
                     NLNLGLNFTGIQETRSGTFTLRAGGIHWVRLSDLTMASFRGYNRFTLFERNPWDPAGA
                     SLPARYETYFNEGGINQDTRWGNRAFKGLILDGNGLPKDLSFTALVGKSEFNGGFSPW
                     PSYTYGGKLQKKTGPGQYISINSLHATTWADSLSEEKVGFHLTTLEWMVHMKGFSLLA
                     EGGLGNYFSPVQDAGNGGLISATLTTPAKKTLPRWSLHYFYISPKVINDNSIFLNTAI
                     REYNPAALPAGTIGSTAVLQPFGSSMLRIGQMSNNRQGADLNMEYTLGDFKFNIGLAA
                     QAELEPISSVITYGHPVNQLTRSRMWRWLFPAEVGPYQRYSVIYRDVYETVNLFDDSS
                     GVVINKKFFSVFEPQVKYHSKVFGKNIYLFYLGYYSSVQRSFSAFTVTSEEAYIRQYA
                     NELEAYLELGDRILISGYAGYERTLGNYLTDIDEESRRPRNQTGTGYGAGLDIDLGFN
                     ARLYLRHRWFAFEDASFADDAFQGQETMVEIKIFF"
     gene            3554..5284
                     /locus_tag="PK742_04330"
     CDS             3554..5284
                     /locus_tag="PK742_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75849.1"
                     /translation="MHKQLTTGIFCTLLCLSTAVQAQQTTPKFTVNGAARSIYFADVL
                     EQELAEPDTVTPPRQNSGHALADIGVNIRPSEDLEIQGMVRIRNDFGGFWGAGVTFDI
                     RQLYLKGVLNKVVRYQLGDINYQLTKYTLYNNDQELYDLSPVLLAHLSDQVMYDNFQN
                     LDHSWRQQGAAVDFGFNFRSVIESADMHLFTSRVMPSNFATVSDRLFSSASIEWVQGA
                     YLSGAFTYASLYDLKGTSRNDILLQNPVYTASLSSEKSFGNWTLSGDLEAGMSQTRYL
                     EDPLAPERSDVFSDLMFQLQDAGGKLSISAGYKWVGPDFRSPGAQTKRVLFDAYPAAF
                     DRLTNNQLVRSLTMLDLIRESSLYNLQLSEGLMVYDPRYDNITPYGAATPNRQGLQAN
                     ATCKLLDDRLSANLNGLMLTEVRGQGTTSYRQFNRLEARLNWEQESYSKNKTRALKAS
                     VSLRMDNTSRPADQEGVPSVDLSTMIAGTGLEIEILEKTDLVAGLLYHSYSGFEFMNQ
                     TDAYGDIVNFNEFNADGSQWMGGAGIRYRFSDQVYVMGQYNNFGTSNVIGTLPAYGVS
                     QLALLFSMEF"
     gene            5287..6291
                     /locus_tag="PK742_04335"
     CDS             5287..6291
                     /locus_tag="PK742_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75850.1"
                     /translation="MKRIALITAISTLLLAGCTRETDLFDGPDLNDLYGEFNLLQALA
                     VNDSEIDFAAGEKAVFTAEFSKNINWTLTITGQESGAVKTITGFSRTLDASNATWDGT
                     TTQLPMFRPEACDVSLTFETEEEVYETSVSVETARPVEGLLLEDFEAGWNPGWGSFVQ
                     SGADMSFVIRTDENAAQGGSYYDMGGEVGWDWLIGLIDIPGSAYGAPTFDLATNPDNI
                     YFNVLLSKQPDLSNALVLFQFREDDNGDGVYTDGEEDMFSLEVALNDQDGWQLISARY
                     ADLVTLVNGAPADPIGNGLNEPDKLIQVSILMLANPVSGYAQALMDLIIFTENGPLKP
                     "
     gene            6288..7487
                     /locus_tag="PK742_04340"
     CDS             6288..7487
                     /locus_tag="PK742_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75851.1"
                     /translation="MSHHERIWLLTLPLVCSLHACSFRTMSVYEQPASADTLSHVGNF
                     YSVDIWNDYITNEFWFSPDTAACLQVTNAFDLNDNGNGSLLLQWNKQAGCSWLGIGFG
                     WDGWASKNLSQIVDRGAIQFMARSSSGNLKGLPWAMALEDYNGAQAWAGVFPEFIDNQ
                     EITGQWTRVQVPLKAFDTKMFHADLSMVKQLIIQLEADGVVYIDDVRVVDLGEHNNRS
                     VDISYNGSSRTLLDQPGAGVQMALTDSSIVLTAVVEDAIHVGTSSEEWPKMENDGLIL
                     AFSSNPDLDNSRKQLFLSDRLLFLPAMKNGLVWDDVNRQTLARPVLFEKTASGYRLTA
                     ELSFRELGISKWEQDENYLLDVAVVDRQSSELVVRYRWNGDDKPAFERNPSTWGSIHV
                     TSELSAK"
     gene            7484..8314
                     /locus_tag="PK742_04345"
     CDS             7484..8314
                     /locus_tag="PK742_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75852.1"
                     /translation="MKATHIAGLFLISLSLSCSQSDPEDVLGRQGDEVNFSGYLWDVK
                     IFENSVMGPGPNYFSGRYEDVWVDENGYLHMHISEHDGRWYSSEVVSRDTMGYGTYTW
                     VVQGDLQNIPENIVLGLFTWDNNTFQTDGNSEIDIEFAKWGNAEAEETLHYSVQPLNF
                     GPYYPERTYDADTPDGSLVGVSTHSFLWTDSIIVWNSYSGEGINPDNLIASWFFDLDN
                     PPRVKNENGISSDPIIIPAPGETTNARINFWIMTWISAGPSDGLEHEVVIRSFSYQAE
                     "
     gene            8311..9738
                     /locus_tag="PK742_04350"
     CDS             8311..9738
                     /locus_tag="PK742_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HQU75853.1"
                     /translation="MRKFLLLAVVWLCVLQVHAQHKNTWQVYETARGNEHLLQQLDDV
                     PTAGSVQPLESERVVFADTTHRFQVHTGMGGAVTDAVAVVYDSLSESTRAAFIQACFD
                     QKNGLGYNLIRVPVHSCDFSPESYTYITEGDSTLQTFSIDHDRTYRFPLLQHIQSVAG
                     KDLLLYASPWSPPAFMKTNKDMLHGGSLLPEYRTAWANYYIRFIQEYEAAGFPVWGLT
                     VQNEPMAVQRWESCIYIAEEERDFVRDHLGPALHAAGMDDKKLIVWDHNRDLLFQRAA
                     TILNDPEASAYVWGVGFHWYETWAGGEPMFENVGLVNSSFSDKALVFTEGCNELFRRE
                     YLGSWAHAERYGEAVIEDFNHGTTAWTDWNLMLNMYGGPNHVGNYCFAPIHADVASDT
                     LLLTPAYYYLGHFSRFIRPGAVRVAAGTNHSDLLTTAYLHPDGSLTVVVMNKSDQPIS
                     YQLQTGQESANMAIPAHAIQTCILR"
     gene            9798..11396
                     /locus_tag="PK742_04355"
     CDS             9798..11396
                     /locus_tag="PK742_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HQU75854.1"
                     /translation="MAAPVNEQPISTGNYRLMLIALIVALGGLLMGFDASVISGVVGF
                     IETKFELTKIQLGWSVSSLTLTSTLAMMVSGPLSNRIGRKKVLTYAAILYAISAIGSA
                     LAPDFVSLVIFRMIGGFGVGASLIIAPMYIAEISPPEMRGRMVSFNQLNIVIGISLAF
                     FTNYLILRYSQSDAAWVKKAHIDTEPWRWMLGLETLPAVLYFFGLFLVPKSPRWLILK
                     GRFEEAKKIMTRFSNAETADREMAEIRQSIEEESKKEHSKQAGVADLFKPALRLVITI
                     GIVVAVLQQITGINSVFFYAPMIFEQSGIGTDASFVQAILVGITNLVFTVLAILFIDR
                     FGRKPLLIVGVAGIVVSMFLLSYGFRAAEYSLSEAAFAKTGITADQASISALYDHSYP
                     DEQAFREAAEQQLGTAFYSEHGSTLSNAALHINPVLILIGILGFVASFAISIGPVMWV
                     LFSELFPNRIRGLAISFVGLINSAVSFIVQLVFPWELEKLGSATTFFIYGVFALLGFL
                     FIWRYVPETKGKSLEALEKELVRS"
     gene            11423..12688
                     /locus_tag="PK742_04360"
     CDS             11423..12688
                     /locus_tag="PK742_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQU75855.1"
                     /translation="MSVLMSCRQTDAQSLPEQVSIRQEGNSYELLVDGAPYTIKGAGL
                     EFGSIAELAEHGGNTFRTWRTDNGVRSAIDILDEAHSYGIKVVMGIDIARERHGFDYN
                     DPVVVQQQLNDVRQQVIALKDHPALLIWGIGNELNLRATNPNVWNAVNDISRMIHEVD
                     PNHLTTTSLAGISADLVKDIRERAPDLDLLSIQMYADIINLPQRIRESGWTGPYLVTE
                     WGATGHWEVQETSWGAPVENNSTVKANYILERYQTGIVPELDRCLGAFIFLWGQKQER
                     TPTWYGLYMEDGKETAAIDAMHFIWNNTWPENTSPAIDSVRLNGMAPGSDIFLHPGDT
                     AEAFSAIRDADGDQLQFQWIIMPESTDLGDGGDFETTPPVITSWISTPDQATTQLVVP
                     NQPGAYRLFLYAFDGHDHAAHANIPFYIK"
     gene            12697..>13972
                     /locus_tag="PK742_04365"
     CDS             12697..>13972
                     /locus_tag="PK742_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75856.1"
                     /translation="MKEQSAGNETMTLQETRRTPEPHLEKILVQGEEFYCIRHYDEMR
                     PFFMNLVSDNNLWMFLSSNGGITAGRINSQQAIFPYYTDDKITDLAPVTGSVTHIRYT
                     DSSFNTHVWSPFAGYILPGYNSERSISKSVLGNRILFEETNHDIQLSFQYGWSFSNRF
                     GIVRTATLVNRGDQEISLDLLDGVQNILPANVGERLQTWRSNLVDAYRKNELLSDPEM
                     AVFSLSAQIVDKPEPSESLRATVAWSLGLENATTLLCGRQIRSFLTGGRVHAEQDIRA
                     ERGAFIKQTRVTLQPGETKEWLILLDIGLGAGEVINLQQLIRNDQNIWQTVLNDTANG
                     QEQLRTLLQAADGIQYSADHLSTARHQSNVLFNILRGGVFTEQYEVRSADFLQYLEQH
                     NKEAAHVYRTRLHNGKRTIPRRYLLDACRIHPS"
BASE COUNT         3599 a         3401 c         3496 g         3476 t
ORIGIN      
        1 tgcagcagtt ctttgaggta gtactgaacc gttcttatga caaagggaat ttcagaaaaa
       61 agcttcatga gatgccttac ctggtagaaa cagagctgtt ccaggaagac gtatcgcatc
      121 gacctgcccg attattcacg tatgatcata ccattcatga aacgcatatc gcatcatagc
      181 cgtttgtcct ggttgctggc ttcactattg ttcctcccag tgcttgtttc tggtcaggca
      241 atacctgtta cggtaggcat gaacagcgaa ggtcagtggc agttataccg caatggtgag
      301 ccatactata taaacggtgt tggaggtaca gaccacctgg atctggcagc agcatgcggt
      361 gccaacagtt tccggacctg gagtccggat gatgccggtg aattacttga tgaagcacaa
      421 cgcctgggta tgacggtcat gcttggccta tgggttcagc atgaaagaca tgggtttgac
      481 tatgacaatg aatccaaagt gaaggctcag ctggactatt tcacagaagt ggtaaagacc
      541 tataaagacc accccgccct cttgctgtgg ggtgttggta atgaagtgga tctgttctac
      601 agcaatacaa aggtctggaa cgccattgaa gatatagctg ccatgatcca tcagattgat
      661 cccaaccatc ctacttctac cgttacggca ggactggatc ctgctgaagt gagactcatc
      721 atggccaacg ctccttctat tgatatatac ggtataaaca catacggaga cattggaatt
      781 gtgcctgata ccatccggtc tgccggctgg caagggccat acatcatcag tgaatggggg
      841 cccaacggac actgggaggt cagcaagaca aaatggaatg ctcctgtaga gcagagcagc
      901 agtgaaaaag ccgcttccta caaagcaaga tataataatt atatagccgg cgacagggtt
      961 aaatgcgtgg ggtcttatgt cttcctgtgg ggacaaaaac aggaaaccac ctctacctgg
     1021 tatggcctgc tctctgaaga cggccgccag tctgaagcca tagatgagct gatcagagcc
     1081 tggacaggaa aggaacctgc caacagggca cctgccattg cttccgcttc gctaaacgga
     1141 cagtcaaaag gtgcagacat cattctgcaa ccagggcaac cctgccaggc actggcaact
     1201 gtgacagatg cagacggcga tcagatgaaa ttcatctggg aagtggtgag tgaaagccag
     1261 gatataaaag ccggtggcga tgcagaatcc cgtccgccct ccgtgaaagg tctgggcatg
     1321 cgaaaagaca gccagcaggt aagtttccga gctccgcaaa gtgaaggtgc ctaccgcctg
     1381 ttcctgtatg tctatgatgc agccaatcat tacgcatacc tgaacatacc tttttatgtc
     1441 ctgcccgatc cttcaggtga gcaataccag aaggcaatcc agctgcggga acagtcgctc
     1501 taaaaaaaat tcaggtacta attgtaaaaa gtacaaaaag tagtacattt atcattccaa
     1561 cagaactgat cggtatgtca ggaacccgta cgtctaatct gaaaatgttt gtatcaggca
     1621 gctttatcat gtggctgata ctgatctgta tgatggctgc caacacagct gtagcacagg
     1681 tagaagacag tacggaaact gcattgggca tccgtcctgc agaagaacca tcgccgctca
     1741 gaaagatctc tgtgtcagga ttttaccgct tctttgccac ctataccaca catgaccttc
     1801 cctatgtgct gaatcctgtt attaatgata ctgtattgcc agccagtctc ttcattggtg
     1861 atgacagcca gcttccaaat ctgcaactca ccatctccgg gaaacctgct ccgcatactt
     1921 cattttcatt tgatgtgttt gcctttcagt ttctcaatgg tgccatcggc accacctatg
     1981 gggcacaagt gccggattca cttcgtccct cttttcagga tccattaagc ggaacacgcc
     2041 ttggcggaca gatgaacctg aatctcgggc tgaactttac cggtatccag gaaacccgct
     2101 ccggcacatt taccttgcgg gcaggcggca ttcactgggt gcgtttgtct gacctgacca
     2161 tggcttcttt caggggatat aatcgcttta cactgtttga acgcaatcca tgggatccgg
     2221 caggagcttc tttgccggcc cgctacgaga cctatttcaa cgaaggtggt atcaaccagg
     2281 atacccgttg gggaaaccgg gcattcaagg gtttgatact ggatggaaac ggccttccca
     2341 aagacctttc ctttactgcc ctggtgggta aatctgaatt caacggaggc ttcagtccct
     2401 ggccaagtta tacttatggg ggaaaactgc aaaagaaaac aggacccgga cagtacatca
     2461 gcatcaacag tttgcatgct accacctggg ccgatagtct gtcggaagaa aaggtaggat
     2521 ttcacctcac caccctggag tggatggtgc atatgaaagg attttcattg ctggccgaag
     2581 gtggactggg aaattacttc agtccggtgc aagatgccgg caacggaggg ctgatatccg
     2641 ctaccctgac cacacctgcg aagaagacat tgccccgatg gagcctgcac tatttctata
     2701 tcagtccgaa ggtcatcaat gacaattcca tttttctcaa tactgctatc cgagagtaca
     2761 atcctgcagc attacctgcc ggaacaatag gcagcactgc ggtattgcag ccattcggca
     2821 gttctatgct gcgcatcggt cagatgtcta ataaccgaca gggagcagat ctgaatatgg
     2881 aatacaccct gggtgatttt aaattcaata tcggtctggc cgcacaggca gaactggaac
     2941 ccatttcttc ggtcatcact tatggacatc ctgtcaatca actgaccaga agccgcatgt
     3001 ggcgctggct gtttcctgca gaagtggggc cttaccagcg ttattcggtc atataccgcg
     3061 atgtctatga gaccgtaaac ctctttgacg acagttccgg agtggtcatc aacaagaagt
     3121 tcttcagcgt atttgaaccg caggtaaaat accattccaa agtattcggc aagaatattt
     3181 acctgttcta tctgggatac tattcttcag tacaacgatc gttctcagcc tttacggtta
     3241 ccagcgaaga agcttacatc aggcaatatg ccaatgaact ggaagcctat ctggaactgg
     3301 gcgaccggat actgatttct ggctatgcag gctatgaaag aaccctgggc aactatctga
     3361 cagatattga tgaagaaagc agaagacccc gaaatcagac cggtacagga tatggcgccg
     3421 gcctggatat cgatctggga tttaatgccc gcctgtatct gcgtcaccga tggttcgcat
     3481 ttgaagatgc cagttttgcc gatgatgcat ttcagggcca ggaaactatg gtagaaatta
     3541 aaatattttt ctagtgcata aacaattgac cacaggaatc ttctgtacgc tgctctgcct
     3601 ttcaaccgca gtgcaggcac aacagacaac tcccaagttc accgtgaatg gtgccgcccg
     3661 ttccatatac tttgctgatg tgctggaaca ggaactggca gagcccgaca cggttactcc
     3721 accccggcag aacagcggac atgcgctggc tgatatcggt gtgaatatcc gtccgtctga
     3781 agatcttgag atccagggaa tggtgcgcat ccgcaatgat tttggtggat tctggggtgc
     3841 gggtgtcaca ttcgatatac gccagcttta ccttaaaggt gtgctgaaca aagttgtgcg
     3901 ctaccagctg ggcgatatta attatcagtt gaccaaatac accctctaca acaacgatca
     3961 ggaactatat gacctgtcgc ctgttctgct tgcacatctg agcgatcagg tcatgtacga
     4021 taatttccaa aatctggatc acagctggag gcagcagggc gccgcggttg acttcggatt
     4081 caacttccgg tctgtcatcg aatcggcaga tatgcacctc ttcaccagca gggtcatgcc
     4141 gtccaatttt gccacggtaa gcgaccgcct gttcagctca gccagtattg aatgggtgca
     4201 gggagcgtat ctgtcaggtg catttaccta tgccagtctg tatgacctga agggtacttc
     4261 acgcaatgat attctgttgc agaaccctgt gtacaccgca tcattgagca gcgaaaaaag
     4321 ctttggcaac tggacccttt ccggcgatct ggaagcaggt atgtcgcaaa cccgctacct
     4381 ggaagatccg ctggcacctg aacggtctga tgtcttttct gacctgatgt ttcaactgca
     4441 ggatgccggg ggtaaactga gcatctctgc agggtacaaa tgggtaggac cggatttcag
     4501 aagtccgggt gcacagacca agcgtgtgtt gtttgacgcc tatcctgcag cctttgatcg
     4561 tcttaccaat aaccagctgg tgcgttctct gaccatgctg gacctgatcc gtgaatcatc
     4621 attgtacaac ctgcaactca gcgaaggtct gatggtctat gatccacgat atgataacat
     4681 cactccttac ggtgcagcca cacccaacag acaggggctg caggcaaacg ctacctgcaa
     4741 actgctggat gaccggctga gtgccaacct gaatggactt atgcttactg aggtaagggg
     4801 tcagggaacc accagttaca ggcaattcaa caggcttgaa gcacgtctga actgggagca
     4861 ggaaagctat agtaaaaaca aaacgagagc attgaaggct tccgtttctt tacggatgga
     4921 taacacctcc aggccggcag atcaggaagg cgttccatct gttgatctca gcacgatgat
     4981 agctggcacc ggactggaaa tagagatcct ggagaaaaca gatctggtag ccggattgtt
     5041 atatcattca tacagtggtt ttgaattcat gaaccagaca gatgcctacg gagatattgt
     5101 caactttaat gaattcaatg cagatggatc tcagtggatg ggtggcgcag gtatccgtta
     5161 ccgtttcagc gatcaggtgt atgtcatggg gcagtacaac aactttggca ctagcaatgt
     5221 cattggcaca ttacctgctt atggcgtttc tcagttagca ctcttattct caatggaatt
     5281 ctgaatatga aaagaatagc acttataacc gcaataagca ccctccttct ggcaggatgt
     5341 acccgtgaaa ctgatctgtt tgacggtccg gatcttaatg atctctatgg cgaattcaac
     5401 ctgttgcagg cacttgccgt aaatgattca gagatcgatt tcgccgctgg tgaaaaagcc
     5461 gttttcaccg cagagttctc gaagaacata aactggaccc tgaccatcac cggacaggaa
     5521 tccggtgctg tgaagacaat aaccggattt tccaggaccc tggatgcatc aaacgctacc
     5581 tgggatggca ccaccaccca gttgcccatg ttcaggccag aagcatgtga cgtatcactc
     5641 acttttgaaa cagaagaaga agtatatgaa acttccgttt ctgtagagac cgcccgacct
     5701 gtggaaggcc ttctgctgga agattttgag gccggctgga accctggatg gggaagcttc
     5761 gttcagtcag gtgctgacat gagctttgtg atccgtaccg atgaaaatgc cgcacagggc
     5821 ggatcctact acgatatggg aggagaagtg ggctgggact ggctcattgg tctgatcgat
     5881 attccgggaa gtgcttatgg tgctccaacc tttgacctgg caaccaatcc ggataatatc
     5941 tatttcaatg tgttgctgtc caaacagccc gacctgagca atgcactggt gctgttccag
     6001 ttccgggaag atgataatgg cgatggtgta tatactgatg gagaagaaga tatgttctca
     6061 ctggaagtag cattgaatga tcaggatggt tggcaactga tctctgcacg ctatgctgat
     6121 ctcgtcacgc tggtgaatgg cgctcccgcc gatccaattg gaaatggtct gaacgaaccg
     6181 gataaactga tacaggtgag catactgatg ctggccaatc cggtttccgg ttatgcgcag
     6241 gcactcatgg atctcatcat cttcaccgaa aacggtccgc tgaaaccatg agccatcacg
     6301 aacgaatctg gttgctgact ttgccgcttg tatgcagcct gcacgcctgt tccttccgga
     6361 ccatgtctgt atatgaacag cccgcttccg cagacacact gtctcatgtt ggcaatttct
     6421 acagcgttga tatctggaac gattacatca ccaatgagtt ctggttctct cctgataccg
     6481 cagcttgtct gcaggtaacc aatgcatttg atctgaatga caatggcaac ggttcactgt
     6541 tgcttcaatg gaataagcag gccggttgca gctggcttgg aatcggtttt ggatgggatg
     6601 gctgggcctc caagaatctg agtcagatcg ttgaccgggg tgctatacaa ttcatggcac
     6661 gcagctcttc cggaaacctg aaaggactgc cctgggccat ggcactggaa gactataacg
     6721 gggcacaggc atgggcgggt gtgttccctg aattcataga caaccaggaa ataaccggcc
     6781 aatggacaag ggtacaggta cccctcaaag catttgatac gaaaatgttc catgcagacc
     6841 tcagcatggt caaacaactc atcatccagc tggaagcaga tggagttgtg tacattgatg
     6901 atgtacgtgt agtggacctg ggtgagcaca acaatcgctc agttgatatc agttacaacg
     6961 gtagtagccg gaccctgctc gaccaacccg gtgccggtgt tcagatggct ttgacagaca
     7021 gcagtatcgt gctgacagcg gtagtagaag atgccataca tgttggcacc tcttctgaag
     7081 aatggcccaa aatggaaaac gatggactca tcctggcgtt ttcttccaat ccggacctgg
     7141 ataacagcag aaaacaactg tttctgagcg accggttgct gttcctgcct gccatgaaga
     7201 acggattggt gtgggatgac gtgaaccggc aaacccttgc acgtccggtg ctttttgaaa
     7261 aaaccgccag cggttaccgt ttaacggcag aactgtcatt ccgtgagctg ggtatcagca
     7321 aatgggaaca agacgaaaac tatctgctgg atgtggccgt agtggaccgt cagtcatcag
     7381 aactggtggt caggtatcgt tggaacggcg atgacaaacc ggcatttgaa cgcaacccct
     7441 ctacctgggg aagcattcat gtgacttcag aactatcagc aaaatgaaag caacacatat
     7501 agcaggtctg ttcctgatct ccttaagtct gtcctgttcg caatctgatc cggaagatgt
     7561 gctgggacgt cagggcgacg aagtgaattt ctcaggctac ctctgggatg tcaagatctt
     7621 tgagaacagt gtcatgggtc ctggcccgaa ctatttctcc ggtcgttacg aagatgtctg
     7681 ggtagatgaa aatggttatc tgcatatgca catttctgag cacgacggcc gctggtattc
     7741 ttctgaagta gtgagccgtg ataccatggg ttacggaact tatacctggg tggtgcaggg
     7801 agatcttcag aatattccgg agaacattgt attgggatta ttcacctggg ataacaatac
     7861 ctttcaaacc gatggcaaca gtgagatcga tattgaattt gccaaatggg gaaatgcaga
     7921 ggccgaagag acactgcatt attctgttca acccctcaac tttggaccgt attatccgga
     7981 acgtacctac gacgctgaca cacctgacgg aagtctggta ggtgtgagta cgcattcttt
     8041 tctttggacc gatagcatca tcgtctggaa cagttattcc ggcgaaggta tcaaccctga
     8101 taacctcatt gcttcctggt tttttgatct ggacaatccc cctcgcgtca agaatgagaa
     8161 cggtatctct tcagacccca tcatcattcc tgcacccggc gaaaccacca atgcacgtat
     8221 caatttctgg atcatgacct ggataagtgc cggtccgtct gatggcctgg agcatgaggt
     8281 cgtgatacgt tccttcagct atcaggcaga atgagaaaat ttttactgct tgcagtagta
     8341 tggctgtgtg tgcttcaggt acacgcccag cacaagaaca cctggcaggt ctatgaaacc
     8401 gccaggggca acgaacatct gttgcagcag ctggatgatg tacccactgc agggtctgtc
     8461 cagccactcg aatcagaaag agtggtcttt gcagatacta ctcatcgttt tcaggtacat
     8521 accggtatgg gcggtgctgt aacagacgct gttgctgtgg tctatgattc actcagtgaa
     8581 agtacacgtg ctgcattcat acaagcctgc tttgatcaaa aaaacgggct tggatacaac
     8641 ctgatcagag tgcctgtaca cagctgtgat ttctcgcccg aaagttatac ttacattacc
     8701 gaaggcgaca gtactctgca gaccttttcc attgatcatg accggaccta tcgttttccc
     8761 ctgctgcagc atatacagtc tgttgcaggt aaggacctgt tgctgtacgc aagcccctgg
     8821 agtccgccgg ccttcatgaa gacgaacaag gatatgctgc acggcggaag tttgttgcct
     8881 gaataccgta ctgcctgggc caattattat atacgtttca ttcaggaata tgaagcagcc
     8941 ggctttccgg tatggggtct gacggtacaa aatgaaccca tggctgtgca acgctgggaa
     9001 tcctgtatct atatcgccga agaagaacgt gattttgttc gtgatcatct gggtccggca
     9061 ttacatgcag caggtatgga tgataagaag ctgatcgtgt gggatcacaa ccgtgacctg
     9121 ctgttccaaa gagctgctac catcctgaac gatccggaag cttctgcata tgtgtggggg
     9181 gttggttttc actggtatga gacctgggcc ggtggtgagc ccatgtttga gaatgtgggc
     9241 ctggtgaaca gctcattctc cgataaagca ttggtcttta ctgaaggatg taatgagctg
     9301 ttcagacgcg aatatctggg tagctgggca catgccgaac gatatggcga agctgtcata
     9361 gaagacttca accatggtac cactgcctgg accgactgga acctgatgct caatatgtat
     9421 ggcggcccta accatgtggg caattactgc tttgctccga tccacgcaga tgtagcatca
     9481 gacaccttgc tgctgacccc ggcctattac tacctggggc atttttcccg ttttataaga
     9541 cccggtgcag tacgggttgc agccggtacc aatcacagtg atctgcttac caccgcttac
     9601 ctgcatccgg atggcagcct taccgtagtg gtcatgaaca aaagcgatca gccgatctcc
     9661 taccagcttc aaaccggtca ggaatctgct aatatggcca ttcccgccca tgccattcaa
     9721 acctgcatac tccggtagtt ttttgtgcaa taagtaggaa aattcctact tatagttatt
     9781 tttacaaaaa gctacttatg gcagcacctg tgaacgaaca acccatcagc acaggcaact
     9841 atcgcctgat gctgatagcc ctcatcgtag cccttggcgg actgctcatg ggattcgacg
     9901 catcggtcat ttccggtgtg gtgggtttca tcgaaacgaa atttgaactg accaagatcc
     9961 agctaggatg gtctgtcagt tcacttaccc ttacctcaac gctggccatg atggtaagcg
    10021 gacctttgag taaccgcatc ggcaggaaga aggtgcttac ctatgctgct attttatatg
    10081 cgatatcggc catcggctct gccctggctc ctgactttgt gtcgctggtc atcttccgca
    10141 tgattggtgg ttttggtgtg ggtgcttcgc tcattattgc acctatgtac attgcagaga
    10201 tcagtccgcc cgaaatgcgt ggccgcatgg tctcattcaa ccagctcaat atcgtgatcg
    10261 gaatttccct ggcattcttc accaattacc tgattctgcg ttacagccag tctgatgctg
    10321 catgggtaaa aaaggcacat attgataccg agccctggcg ctggatgctc gggttggaaa
    10381 cattgcctgc tgtgctgtac ttcttcggac tatttcttgt accgaaaagt ccgcggtggc
    10441 ttatcctgaa gggtcgattc gaagaagcca aaaagatcat gacccgattc tccaatgccg
    10501 aaacagcaga tcgcgaaatg gcagagatcc gtcagagcat cgaagaagaa agtaaaaaag
    10561 aacacagcaa acaggcaggc gtagctgacc tgttcaaacc ggcattgcgc ctggtcatca
    10621 ccatcggcat agtagtagct gtactgcaac agatcacagg tatcaattcc gtattctttt
    10681 atgcacccat gatatttgaa caatcaggca tcggcacaga tgcttctttc gtccaggcca
    10741 ttctggtagg tataaccaat ctggtattca ctgttctggc cattctgttc atagacaggt
    10801 ttggccgcaa gccgctgctc atcgtgggtg tggccggaat tgtggtgtcc atgttcctgt
    10861 tgtcttacgg atttcgtgct gcagaatatt ccctgtcaga agctgcgttt gccaaaacag
    10921 gcattaccgc agatcaggca tccatctctg ctttatatga tcattcctac cctgatgaac
    10981 aggcttttcg ggaagcagca gaacaacagc ttggtactgc attttactct gaacatggca
    11041 gtacactttc caatgcagcc ctgcatatca atccggttct gatcctgata ggtattctgg
    11101 gcttcgtggc atcctttgct atttccattg gaccggtcat gtgggtattg ttctctgaat
    11161 tgtttcccaa ccgaattcgc gggctggcta tttcctttgt aggactgatc aacagtgctg
    11221 tgagttttat tgtacaactg gtgtttccct gggagctgga aaagctgggc agtgcaacca
    11281 ccttcttcat ttacggtgta ttcgctttgc tgggtttcct gttcatctgg cgttatgttc
    11341 ctgaaaccaa aggcaaaagc ctggaagcac tggagaaaga gctggtgaga tcatgatatc
    11401 aagatcatta tatattattt cattgtctgt attaatgtca tgccggcaaa cagatgccca
    11461 gagtttacct gaacaggtat ccatcaggca ggaaggtaac agctatgaac ttttggtgga
    11521 cggggcaccc tataccatca aaggagcagg tctggaattt ggcagtattg ctgaactggc
    11581 agaacacgga ggaaatactt tccgcacctg gcgtacagat aacggagtgc ggtcagccat
    11641 tgacatcctg gatgaagcac attcatatgg catcaaagtg gtgatgggta ttgatatcgc
    11701 ccgtgagaga catgggtttg attacaatga ccccgttgtg gtgcagcaac agctgaatga
    11761 tgtcaggcag caggtcatcg ccctcaaaga tcatcccgct ctgttgatat ggggcatagg
    11821 caatgaactg aatctgcgtg ctaccaatcc gaatgtctgg aatgcggtca acgatatcag
    11881 ccgcatgata catgaagtgg atccgaatca cctgaccact acctcactgg ccggtatcag
    11941 cgcagatctg gtcaaagata ttcgcgagcg tgcgcctgat ctggatctgc tcagcatcca
    12001 gatgtatgca gatatcatca acctgccgca gcgcatcaga gaatccggct ggaccggacc
    12061 atatctggta actgaatggg gagctaccgg tcactgggag gtgcaggaaa cctcctgggg
    12121 tgcaccagta gagaacaaca gtaccgtaaa agccaactac atactggaac gttaccaaac
    12181 cggtatcgtt ccggaactgg accgttgtct gggtgcattc atttttttgt ggggacaaaa
    12241 acaggaacgc acacccacct ggtacggact ctacatggaa gacggaaaag aaacggcagc
    12301 gatagatgcc atgcatttca tatggaacaa cacctggcct gaaaacacca gtcctgcaat
    12361 agattctgta agactgaacg gcatggcccc cggcagtgat atttttctgc atccgggcga
    12421 tacggcagaa gcattttctg ccatcagaga tgccgatggt gatcagcttc aattccaatg
    12481 gatcatcatg ccggaaagta cagacctggg tgatggcggt gattttgaaa ccactccgcc
    12541 tgtcattacc agctggatat caacacctga tcaggcaacc acacaactgg tggttccgaa
    12601 ccagcccgga gcataccgtt tgtttctgta tgcttttgat ggacacgacc atgctgcgca
    12661 tgccaatatt cctttctata ttaaatgacc ctatccatga aggagcagag tgcaggaaat
    12721 gaaaccatga cattgcagga aacacgcagg acaccggaac ctcatctgga aaagatcctg
    12781 gtgcagggtg aagaattcta ttgcatcagg cactatgatg agatgcgccc cttcttcatg
    12841 aacctggtaa gcgacaacaa cctctggatg ttcctgtcca gtaatggcgg catcactgca
    12901 ggacgcatca attcacagca agctatcttt ccctactata ctgatgacaa gatcacagat
    12961 ctggcacccg taaccggaag tgttacccat atcaggtaca ccgactcttc tttcaacacg
    13021 catgtatggt cgccgtttgc aggatatatc cttccaggct acaactcaga acgcagcatc
    13081 agtaaaagtg tactgggaaa caggatcctg ttcgaagaaa caaatcacga catccaactc
    13141 agctttcaat acggatggtc tttttccaat cgctttggca ttgtcagaac agccactttg
    13201 gtcaaccggg gtgatcagga gatctctctg gatctgctgg atggcgtaca gaatattctg
    13261 cctgcgaatg tgggcgaaag acttcagacc tggcgcagta atctggtgga tgcataccgg
    13321 aaaaatgagc tgttatctga tccggaaatg gctgtgtttt ctctgagtgc ccagatcgtt
    13381 gataagcctg agcccagtga atcactgagg gctacagtgg cctggagcct tgggctggaa
    13441 aatgcaacca ccctgttatg cggtcgtcag attcgcagtt ttcttaccgg aggccgggtt
    13501 catgctgagc aggatatacg tgcagaacgc ggtgcattca tcaagcagac cagagtcaca
    13561 ttacagcccg gtgaaacgaa agaatggctg atcctgctgg acattggcct gggtgccggc
    13621 gaggtcatca acctgcaaca gctcatcaga aacgaccaga atatttggca aaccgtgctg
    13681 aatgatacag caaacggaca ggaacagctg cgcaccctgc tgcaagctgc agatggtatt
    13741 caatactctg ctgatcatct gagcacagca agacatcagt caaacgtgct gttcaatatc
    13801 cttaggggtg gagtgttcac agagcaatat gaggtgcggt cggcagattt tctgcagtat
    13861 ctcgaacagc ataataaaga agcagctcat gtgtaccgta cccggttgca caacggaaaa
    13921 cgaaccatcc cccgccggta tctgctggat gcctgccgta tccatccttc tc
//
LOCUS       DAOMAX010000050        13608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_543_length_13608_cov_4.188372, whole genome shotgun sequence.
ACCESSION   DAOMAX010000050 DAOMAX010000000
VERSION     DAOMAX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_543_length_13608_cov_4.188372"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..557)
                     /locus_tag="PK742_04370"
     CDS             complement(33..557)
                     /locus_tag="PK742_04370"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQU75857.1"
                     /translation="MNDQQAKKVSESTTIFTEIVMPNDTNPMNNLMGGNLLKWMDIAS
                     GICAGKHAGKVAVTAAVDNVSFGRPIKIGDVVTIEAQVTRAFNSSMEIYIEVFKENIR
                     SRSAKVKCNEAYYTFVALDDDGRPCAVPPVDPQTDEEKHRFERAMRRRELRLILAGRM
                     KPEEAAELKAIFIP"
     gene            580..1437
                     /gene="ygiD"
                     /locus_tag="PK742_04375"
     CDS             580..1437
                     /gene="ygiD"
                     /locus_tag="PK742_04375"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HQU75858.1"
                     /translation="MHRRQFIQNSLMAMTALQLKDLTELLPTGYDGVMPAMFVGHGSP
                     MNAIEENAFSNEWERIGKQLPLPQAILCVSAHWMTNGTKVAMTDKPETIHDFYGFPEE
                     MYRINYPAPGSPAWALTVKDTVQTVSVQEDHDWGLDHGSWSVLKKMFPEANVPVFQLS
                     LDAKASPEQHYALARELSGLRERGLLIVGSGNIVHNLRMAQWNDSAYDWTIEFDEQVK
                     ELINKGDHQSLVAFRKLGRAADLSIPTAEHYLPMLYALGASLPGDDLFYFNEKNTMGS
                     VSMRSFVLG"
     gene            1500..2690
                     /gene="sucC"
                     /locus_tag="PK742_04380"
     CDS             1500..2690
                     /gene="sucC"
                     /locus_tag="PK742_04380"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQU75859.1"
                     /translation="MNLHEYQGKELISRFGVRIQQGIVAKTVDEAVAAAKKLHEDTGT
                     SIWVVKAQIHAGGRGKAGGVKVAKSLEEVAEKAGAILGATLVTPQTGAHGKLVNQVLI
                     AQDVYYPGAEDTKEFYVSVLLDREKSRNVIVYSTEGGMDIEEVADKTPHLIHKEWIDP
                     AVGIMPFQARKVAFNLGLSGESFKEMTRFVTSLYNAFTGNDCSLLEINPVLKASDGKI
                     IAVDTKCVVDNNALGRHADIAAMRDLTEEDPTEVEAGEAGLNFVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGNPANFLDVGGTADAQRVETAFKIILKDPEVKAILVNIFGGIVRC
                     DRVAQGIIDAYKNIGEISVPIIVRLQGTNAEEAKQMIDESGLKVKSAILLKEAAALVQ
                     EAFA"
     gene            2704..4185
                     /locus_tag="PK742_04385"
     CDS             2704..4185
                     /locus_tag="PK742_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQU75860.1"
                     /translation="MYQVSAWETYFKPFHDRIIGVDETFSGPYGEKKIIYADWTASGR
                     LDRKIEEKFLREIYPIVANTHTDTNYTGSAMTRAYHHAMGIIKAHVHAGPDDVLISSN
                     SGMTGVVNKLQRILGFKLHEDFRNKVSLTPDERPVVFITHMEHHSNQTSWLETIAEVR
                     IIDPDENGLVDLKHFAALLEEYRDRPLKIAAVTSCSNVTGIMTPYHTMAGMIHRAGGY
                     CFVDFACSAPYIDINMHPDEQDEYLDAVYFSPHKFLGGPGAPGAVVFNKKLYHTSVPD
                     HPGGGTVKWTDPWGYHLYLDDIELREDGGTPAFLGTMRTALSILLKEEMGTEQIAARE
                     KEIMDMLWPRLKAITNLHILAGEIRERLPVISFYIDDLHYNLVVRMLNDRFGIQVRGG
                     CACAGTYGHYLLYIDSQNSEEIANEILSGDFSHKPGWVRLSLHPTTTNDELEYILQSI
                     ESLCLHASEWMEDYDCDLQHLEIRNKVLTTDPSEITARRLLQL"
     gene            4264..5631
                     /locus_tag="PK742_04390"
     CDS             4264..5631
                     /locus_tag="PK742_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQU75861.1"
                     /translation="MEQIIQSLRSIPADRISFEEVGRVLYQTDPASYPYAEHLPEKVT
                     TGYSRKIVTLDPIECLVLYWSPGAASAVHFHEGFWGYVAVVRGICQNVEYAMKDGILR
                     ETSITTVHAGGIVPEQDNIIHTIRNGSETQPLITVHFYHPALVNLDGLQIYDLANGRI
                     GILNDQAASASWDEPVSSFSRITDHAFSYDTSGDDEPASHIIRPLIPKPDATTISNML
                     EAYYDDQATMYDYLDQAYASRKLYIEGINSRIAGHLQQAGNVDRLLAMACGTGRRATE
                     IRTLSGQDYQIIGVDLSKEMVEQAKDRDLEIMHASWNDFELHLSGKFDILTTLYAFGH
                     LSCHEVRVDYLRRLLRLAKQGALLFVDVFNLDDQTEWGPLIREMHRKLHLKHFNYESG
                     DVFYSRNRHDHLAYLHYFTETEIRKLVAESGWKIRSLEYVGYSNHPGETSSSDSGNIF
                     LVLEA"
     gene            complement(5632..6519)
                     /locus_tag="PK742_04395"
     CDS             complement(5632..6519)
                     /locus_tag="PK742_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQU75862.1"
                     /translation="MRNRATGFVLMSTMFFALMNVCIKLLPGIPSYEIVLFRSVISFI
                     LSLTLLLRLRIFPFGKGNYFYLIMRGIAGSAALLIYFYTLQHLPLATAVTVQYLSPLF
                     TVLFAAFLLKEKLRWQQWIFLLLSFAGVVLIKGFGNDFPVSLLLLGMLSAALTGFAYN
                     AVRKAGETEHALVVVFYLPLVTIPVITPFAVNNWVMPSGNEWLLLIAVGLLTQIAQVY
                     QTRAYQIEMASRVTPYTFLGIVFAIIFGIFIFHEHYALTGYLGMIIVMTGVVLNFFFL
                     NRLTSLRRLQAYLRHFPGG"
     gene            6656..8266
                     /locus_tag="PK742_04400"
     CDS             6656..8266
                     /locus_tag="PK742_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU75863.1"
                     /translation="MYNSAKSLLFILLLTGASVFAQTASDTTMTMQEAQLKLERFMQL
                     IDSKYLEDVDPGVMVDEAINAILEHLDPHSVYFTEEELQQANEPLEGNFEGVGIQFNI
                     LRDTIIVVNTISGGPSEKVGIMSGDKIVVIDGENVAGIGITNKGVTDRLRGPKGTRVK
                     VSIDRKGLTKPQDFSITRDVIPLYSVDASYMLDPETGYIRVSKFAASTVEEFRDAVSE
                     LKGDGMENLILDLRGNGGGYLNAAIDLSDEFLDAGKLIVYTDGRAYPRQDAYATSFGK
                     MQSGKVVVLIDESSASASEIVSGALQDWDRALIVGRRSFGKGLVQRPYNLTDGSAVRL
                     TVSRYYTPSGRCIQKPYDDGIDAYHKDLDERFQDGELTADHVFEYPDSLKYFTNAKRV
                     VYGGGGIMPDIYVPIDTTWTTEYYLDILRNSLHNQFSLTYVNDHREELNKQYPDAFAF
                     LNGFKVTEGLWKEFIDYATSEEVAFNEEEFETCKERLDLLLRSTIARNLYDFEAFWVV
                     YNSQDEAIDKAMEAMHDETFRKMKIASK"
     gene            complement(8312..13333)
                     /locus_tag="PK742_04405"
     CDS             complement(8312..13333)
                     /locus_tag="PK742_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HQU75864.1"
                     /translation="MIKRIPSLIVLLWFASALSAQTDLYGNEWISYDQQYLKIRTAAD
                     GLYRLHYDELADALTAIGTDIATIAPDDFQLFQLGEEQYLYIEGDGDGSFDPGDFIEF
                     YGIRNDGKLDTRLYENEQYQIHTYTSLFTDTAVYFLTWNSDPGHLRMEQLANVLSDMP
                     EPESHYEFRTLMINGSTTVLNHGASYFAPGRYYLEIYSSKYDDGEGFSETRFAGTTRN
                     KTLSTSNIYSGEDAFVPTLRTAVIATNEVEHHYTISFNGTTLYDTSFTGYDFKKYEFS
                     IDNLLSSNTVSFASLNGSNDYLRNCYIEITYPRIWDFNNASKVGLRLVNSLSATKYLV
                     ITDFNEKSTDPVIWDFDDHKRMIGTIVGSNTEIHMPYDASGNRIWLSSQDTLDILQVQ
                     ELEPVTFIDFSNPVNQGDYLIISHAGLRDDGTGEDWIQAYADYREGFAGGSFQPLIAD
                     IAQLTDQFAYGIKTHPLAVRNFVFFAEDHFETDPRYLFLIGKARKYDGCRNNAESFAQ
                     NTVPTFGNPGSDVLLVCRPGSYIPELPVGRLVAYNGTEVKNYLDKVVEYETVQATTDQ
                     TIESKAWMKNMLHFAGGLNEYEQSLFNTYLRNLGDIAEDTLYGANVIQFNKITTDPIF
                     YETSDYIDSLINSGVSLVTFFGHSTTGSFDYNIGDPEDFTNYGKYNVIFGNGCNTAAI
                     FDGTITLGEKYINTEDRASIAFIAATTFSLAGSLYNYGLRFYAELSRYRYGQGIGDAL
                     QGAAEYISAFDNIYDLLTLQHTALQGDPALRLNTHSLPDYVIEEPYVFFEPSVITTLE
                     DSFTVQVVVTNIGMAIDKDYTVSVERIRPDGSSELVTRTLRSAQFRDTVGIRLFTDAN
                     SGVGLNSFTITVDYGDSIAEIAEWNNVISKTQLILGDDANPVYPYEFALINEAPDYLA
                     ASTSNPFAGTRTYVMQIDTTASFNSPLLQSHSVTQSGGVLKWEAPPVTWLSGRVYYWR
                     VSVDTAGGADYLWRTSSFRYKSGEEIGWNQSHYFQYADDLYDNISLDEDRNFHFVNNY
                     VNYQVATGIYPTTDWTEVTSYLNGEEMAVGSCANNGFVVFVIDPVSGYPWQVSEVDDS
                     GFGPYGDIYCSADAYEQIIQFSTADLESRTAFYHFMKDSVPDGTYFLCYSNNYPQFNE
                     WLDDTLSLGGESLFDAFNEYGAVMINDLAVYDFDRSYIFYAKKGEPASAFEIIGDTLG
                     NKIDAVVNISGLWDEGSYTSERIGPAAAWDQVKWNYEAPDSEADSVYLEVLGEDEYGM
                     QTLLSSGLVSGDTTISWIDANMYPYIRLRTNTFDDVVRTPAQLNEWQVIYTPVGEAAL
                     NAFADFSWQKDSIQQGEPLHFRVAVDNVSAWDLDSMLIAFTIQDAANVLHPVPFERQD
                     SIRAFERLTADITIDTKDIPPGASTLIVEVNPPFDQPEQYHFNNIGYLPLHVSGDLSD
                     PNIDVTFDGVHILDGDIVSASPAIVIALKDENTFLALSDTSLMQVSVKYPDGSVVPFA
                     YHDGTLIFYPAETAATNNTARIEMNPDFSQDGLYELWVNGADVSGNSSGDGVDYRIGF
                     EVVNKPMVSNVLTYPNPFTTQTRFVFTLTGSEVPDYIKVQILTVSGKVIREVLAPELG
                     PLHIGTNITEFAWDGTDKFGDPVGNGLYLYRVVFRLDGQSLEHFDTGTDQYFESGLGK
                     MYLAR"
     gene            complement(13351..>13608)
                     /locus_tag="PK742_04410"
     CDS             complement(13351..>13608)
                     /locus_tag="PK742_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75865.1"
                     /translation="HLGIEAGYKDFIYLRAGVNNVQQAIADDATTESWVLQPNLGVGV
                     VIGPIHLDYAYTNIGNQAEVLYSHVVSLQLSMPSRQVPDQN"
BASE COUNT         3771 a         3236 c         3184 g         3417 t
ORIGIN      
        1 ccataaaaaa agccggcaat agccggcttt ttttatggaa taaatatcgc ctttaactca
       61 gccgcttctt ctggtttcat gcgccctgcc aggatcagac gcaactctct tcgccgcatg
      121 gcccgctcaa acctgtgctt ctcttcatcg gtttgaggat ctaccggagg aacagcacat
      181 ggacgaccgt catcatccag ggcaacgaaa gtataatagg cttcgttgca cttcactttt
      241 gcagatctgc tcctgatatt ctctttgaat acctcgatat agatctccat ggaggagttg
      301 aaagctctgg tcacctgagc ttcaatggtc accacatccc cgatcttaat agggcgccca
      361 aagctgacat tatccactgc agcagttacc gccaccttcc ctgcatgctt tccggcgcaa
      421 ataccactgg caatgtccat ccatttaagc agattacctc ccatgagatt gttcattgga
      481 ttggtatcat taggcatcac gatctctgta aaaatggtcg tagattcgga aacttttttt
      541 gcttgctggt cattcatatt gcatgcaaag gtaccgaata tgcaccgcag acaattcata
      601 caaaacagcc tgatggccat gacagcctta caactaaaag atctgaccga actgctccca
      661 accggatacg acggggtgat gccagccatg ttcgtgggac atggcagccc catgaatgcc
      721 atagaagaga atgctttcag caatgaatgg gaacgcattg gcaaacagct gcccctgcca
      781 caggccatct tgtgcgtatc tgcacactgg atgaccaatg gcacaaaggt ggcaatgact
      841 gacaaacctg aaaccatcca tgatttctac ggttttccgg aagaaatgta ccggatcaac
      901 taccccgcac ccggctctcc tgcctgggct ttaaccgtta aggacacggt gcaaaccgtt
      961 tctgtacagg aagaccatga ctggggtttg gaccacggaa gctggagtgt gcttaaaaaa
     1021 atgttcccgg aagccaacgt gccggtcttt cagctgagtc tggatgccaa agcaagcccg
     1081 gagcaacact atgcactggc ccgggaactt tctggcctcc gggaaagagg attgctcatt
     1141 gttggcagcg gtaatattgt gcacaacctt cggatggcac aatggaatga ttcagcctat
     1201 gactggacga tagaatttga tgaacaggta aaagaactga tcaataaagg tgaccatcag
     1261 tctttggtag cattcaggaa acttggccgt gctgccgatc tttccatacc gacagctgaa
     1321 cattacctgc caatgctcta tgccttaggt gcttccttac ccggcgatga cctgttctat
     1381 ttcaatgaaa aaaataccat gggatctgtt tccatgcgca gtttcgtgtt gggataatct
     1441 ggacctgaag acggtcagcc acggtggcat tgtgtatctt tgcgggcaaa acagttgaga
     1501 tgaatctgca tgaataccag ggtaaagaac tgatcagtcg ctttggcgta cgcatacagc
     1561 aaggcattgt agcaaagacc gttgatgagg cagtagctgc cgccaaaaag cttcacgaag
     1621 ataccggcac ttccatttgg gtggtgaagg cccagatcca cgccggtggc cgtggtaaag
     1681 ccgggggtgt caaagtggcg aaatcactcg aagaagtagc agaaaaagct ggcgctattc
     1741 tcggcgccac attggtaacg cctcagacgg gagcccacgg caaactggta aaccaggttc
     1801 tgattgcaca ggatgtgtac taccccggag ctgaggatac gaaagaattc tatgtttcgg
     1861 ttctgctgga tcgcgagaaa agcagaaacg tgatcgtgta ttccacagag ggcggaatgg
     1921 atattgaaga agtagccgac aaaaccccac atctgatcca caaagaatgg atcgatcctg
     1981 ctgtaggaat catgcctttt caggcccgga aagttgcatt caatctggga ttgtctggcg
     2041 agtctttcaa agagatgaca cgcttcgtga ccagcctcta caatgccttc acaggaaatg
     2101 attgttcact tctggagatc aaccctgttc tgaaagcgtc tgacggtaag atcattgcgg
     2161 tagataccaa atgcgtggtt gacaacaacg ccctgggaag acatgctgat attgcagcta
     2221 tgcgagatct tacggaagaa gatcctacgg aagttgaagc tggagaagcc ggactcaact
     2281 tcgtcaaact ggatggaaat gtaggttgta tggtaaatgg tgccgggctg gcaatggcta
     2341 ccatggacat catcaaactt tccggcggca acccggccaa cttcctcgat gtgggaggaa
     2401 ctgccgatgc acaaagggtg gaaacagcat tcaagatcat tctgaaagat cctgaggtca
     2461 aggccatact ggtcaatata ttcggtggca ttgttcgctg cgaccgtgta gcgcaaggga
     2521 tcatagatgc ctataagaat attggtgaga tcagtgtgcc tatcatagtc cgcctgcaag
     2581 gaaccaatgc tgaagaagcc aaacaaatga tagacgaaag cggcttaaaa gtaaagagtg
     2641 ccatcctgct gaaagaagca gctgcgctgg tgcaggaagc ttttgcttaa gaattctgcc
     2701 tttatgtatc aggtatctgc ttgggaaaca tatttcaaac catttcacga ccgtatcatc
     2761 ggggtggatg agaccttttc cggaccttat ggagaaaaga agatcatcta tgccgactgg
     2821 acagcaagcg gcagacttga tcggaagatc gaagaaaagt ttttacggga gatatacccc
     2881 attgtagcca atacccatac cgatactaat tataccggca gcgctatgac acgggcctat
     2941 caccatgcca tgggtatcat caaggcgcac gtacacgccg gtcctgatga tgtgctgata
     3001 tccagtaact ccggcatgac aggagtggtg aacaagctgc agcgtatcct tggttttaaa
     3061 cttcatgaag atttcagaaa caaggttagc ctgacacctg atgaacgacc tgtagtgttc
     3121 atcacccata tggaacatca ttccaaccag acatcctggc tggaaaccat tgcagaggtg
     3181 cggatcatag accctgatga aaatgggctg gttgatctga agcattttgc tgccctgctg
     3241 gaagaatacc gtgacaggcc gctgaagatc gctgccgtaa cgtcctgcag caatgtaacc
     3301 ggtatcatga caccctatca taccatggcg ggtatgattc accgggctgg tggatattgt
     3361 tttgtggact ttgcctgttc tgctccttat attgatatca atatgcaccc tgatgaacag
     3421 gatgaatacc tggatgcagt gtacttttct ccccataaat ttttaggagg gccgggggca
     3481 cccggagctg tggtcttcaa taagaaactc tatcatacat cagtacctga ccatccgggc
     3541 ggaggaacag tgaaatggac cgatccctgg ggttaccatc tttatctgga cgatattgaa
     3601 ttgcgtgaag atggcggcac tcccgctttt cttggcacca tgcgcacagc cctgagcatt
     3661 ctcctgaaag aagaaatggg aacggaacag atagctgccc gtgaaaaaga gatcatggat
     3721 atgctttggc ccagactgaa ggccatcacc aatctgcata tcctggcagg agaaatacgt
     3781 gaaagattac ctgttatttc cttctacatt gacgatcttc attataacct ggtagtgaga
     3841 atgctcaatg accgtttcgg tatccaggta agaggtggct gcgcctgtgc cggtacatac
     3901 ggtcactatc tgttgtatat agacagccag aacagtgaag agatcgcaaa tgaaattctg
     3961 tctggcgact tttcccataa gccgggatgg gtcaggcttt ccctgcaccc taccacaacc
     4021 aatgatgaac tggagtatat cctgcaatcc atagaatctc tgtgcttgca tgcttctgaa
     4081 tggatggaag actacgactg cgacctgcaa catctggaga tcagaaacaa ggtgctgact
     4141 acagatcctt ccgaaattac agcccgcagg ttattgcaac tctgaaacta cctgttattt
     4201 attatatgtt gtttattaat gtgaaagcat tgacagcttt tgactatttt cattgctccg
     4261 aacatggagc agatcataca atctttgcga tccatcccgg cagaccggat cagttttgaa
     4321 gaagtgggca gggtcttata ccagacagat cctgcttctt atccttatgc tgagcactta
     4381 ccggaaaagg ttaccaccgg gtattcccga aagatcgtaa ccctggaccc tattgaatgt
     4441 ctggtgttat actggagccc gggagctgca agtgcagtgc atttccatga aggattctgg
     4501 ggttatgtgg cagttgtcag gggtatttgc caaaatgtgg aatatgctat gaaagatggc
     4561 atccttcggg aaacatccat cactaccgtt catgcaggag gcattgttcc ggagcaggac
     4621 aatatcatcc acaccatacg gaatggatcg gaaacccagc ccctgatcac agtgcacttc
     4681 tatcatcccg cactggtgaa cctggacggt cttcagatct atgatctggc aaatggccga
     4741 ataggtatcc tgaatgatca ggctgccagt gccagctggg atgagcctgt ttcatctttc
     4801 agcaggatca cagaccatgc tttctcttat gatacgtcag gcgatgatga acccgcatct
     4861 catatcattc gccccctcat ccccaaaccg gatgcaacaa ccataagcaa catgctggaa
     4921 gcctactatg atgatcaggc taccatgtac gattatcttg accaggcata cgcatccaga
     4981 aaactatata ttgaaggaat caacagtcga atagcaggtc atttacaaca ggctggcaat
     5041 gtggaccgtt tgctggcaat ggcatgcgga accggcaggc gggctacaga gatcagaact
     5101 ttgtcgggcc aagattatca gattatcggg gtcgatctca gtaaggagat ggtggaacaa
     5161 gctaaagaca gagacctgga gatcatgcat gccagttgga acgattttga actgcatcta
     5221 agcgggaagt ttgacatact gaccacgtta tatgcatttg gtcatctgtc ctgccatgag
     5281 gtccgggtgg actatttgcg cagattactc cggcttgcca aacagggagc attgcttttt
     5341 gtagatgttt tcaacctgga tgaccagact gaatggggcc cgctgatcag agaaatgcac
     5401 cggaaacttc atctgaagca tttcaactat gaatcaggtg atgttttcta cagcaggaac
     5461 agacacgacc acctggcata cctgcattac ttcacagaaa cggagatcag aaaattggtg
     5521 gccgaaagtg gctggaagat caggtcactg gaatatgttg gatacagcaa ccaccccggc
     5581 gaaacttctt catcagattc aggcaatatt ttcctggtac tggaagcctg atcacccacc
     5641 cggaaaatgc ctgagatagg cctgcaacct gcgtaaactg gttaacctgt tcagaaagaa
     5701 gaaattcagc acgacaccgg tcatcactat gatcatcccc agatatccgg tcagcgcata
     5761 gtgctcatga aatatgaaga tgccaaagat gatggcgaaa acgatcccca gaaaagtata
     5821 aggcgttacc ctgctggcca tctcaatctg atatgccctg gtctgataca cctgggcgat
     5881 ctgtgtcagc aacccgacag caattaacaa cagccattcg ttgcccgaag gcatcaccca
     5941 gttgttcacg gcaaaaggcg tgatgaccgg aatggtgacc aatggcaaat agaacaccac
     6001 aaccagtgca tgctcagtct cgcccgcctt tctgactgca ttataggcaa agccggtaag
     6061 cgccgccgac agcataccca gtaacaacag agaaacggga aaatcattgc caaacccttt
     6121 tatcagcacc accccggcaa aagagagtag taaaaagatc cattgttgcc agcgaagctt
     6181 ctctttcagc agaaaagcag cgaacagaac agtgaaaagc ggcgacagat attgcactgt
     6241 gactgcagtg gccagtggca ggtgttgcag cgtataaaaa taaatcagca aggccgctga
     6301 accagctatt ccccgcatga tgaggtaaaa gtaattgccc ttcccgaacg ggaatatcct
     6361 gagacgcagc aacagagtaa gtgataagat gaaagagatc acagaacgaa acaacactat
     6421 ttcatagctg ggtattccag gcaacagctt gatacacacg ttcatcaggg cgaagaacat
     6481 cgttgacatc aatacaaagc ctgttgccct gttacgcatg tcacaaaggt aaacaaccgc
     6541 cggatgcagc agctttccgg gatttatggc atgaaatgtg aaatactttt aaaaattgcg
     6601 tcttatcata aaaacttgct tcctttaacg ttgataattt gttaagccta tcattatgta
     6661 caacagcgca aaatctcttc tattcattct gttactgacc ggggcatctg tgttcgcaca
     6721 gaccgccagc gacactacca tgaccatgca ggaagctcag ctcaagctgg agcgtttcat
     6781 gcagctcata gattccaaat acctggaaga tgtggacccg ggcgtaatgg tcgatgaggc
     6841 catcaatgcc attctggaac acctggaccc ccactctgtc tattttaccg aagaagaatt
     6901 acagcaggcc aacgagccgc tggaaggcaa ttttgagggt gtgggtattc agttcaatat
     6961 tcttcgagat accatcatcg tagtgaatac catttcaggc gggccatcag aaaaagtggg
     7021 catcatgagc ggcgataaga tcgtggtgat agacggcgaa aatgtagccg gaattggcat
     7081 caccaacaaa ggagtgaccg acagacttcg cggaccaaaa ggcaccaggg tgaaggtcag
     7141 tattgaccgc aaaggcctga caaaacctca ggacttttcc attacccgag atgtgatacc
     7201 actatacagc gttgatgcat cctatatgct ggatcctgaa acaggctata tccgcgtaag
     7261 caaatttgcc gcatctactg ttgaagaatt ccgtgatgct gtgtctgaac tgaaaggcga
     7321 tggcatggag aacctgatcc tggatcttcg cggaaacggc ggaggatatc tgaatgcagc
     7381 catcgatctg agcgatgaat ttctggatgc cggaaaactt atcgtttata ccgatggccg
     7441 ggcataccca agacaggatg catatgctac ctcatttgga aaaatgcaat ccgggaaagt
     7501 agtggtgctc atagatgaat cgtcggcttc tgccagtgag atcgtttccg gagccctgca
     7561 agactgggat cgtgccttga tcgttggaag gcgcagtttt ggaaaaggtc tggtgcagcg
     7621 tccgtacaac ctgaccgacg ggtctgctgt cagacttact gtaagcagat actatactcc
     7681 ttctggcagg tgtatccaaa agccttatga cgatggaata gatgcttatc acaaagatct
     7741 cgatgaacgc tttcaggatg gagaacttac cgccgatcat gtatttgaat acccagactc
     7801 cctgaagtat ttcaccaatg caaaacgggt ggtttacggc ggaggtggta tcatgcctga
     7861 tatctatgta ccaatagata ctacctggac cacagagtac tatctggata tactgcgtaa
     7921 cagcctgcac aaccagttca gtctgacata tgtaaatgac caccgcgaag aactgaataa
     7981 acaatatccg gatgcctttg catttctgaa tggttttaag gtgaccgaag gactttggaa
     8041 agaattcatt gattatgcaa cttctgaaga agtagcattc aatgaggaag agtttgaaac
     8101 ctgtaaggaa agacttgact tgttacttcg ttctaccatc gccagaaatc tgtatgattt
     8161 tgaagccttc tgggtggtct ataactctca ggatgaggcg atagacaaag caatggaagc
     8221 tatgcatgac gaaaccttcc gtaaaatgaa gatcgcttct aaataatagc ttcaagataa
     8281 aaaaagcccc gttgtttaac ggggcttttt tttatctggc cagatacatt ttacccagac
     8341 cgctctcgaa atactgatcg gttcctgtat caaagtgctc cagtgactga ccgtccagtc
     8401 tgaaaaccac acggtacaga tagagtccgt ttccaaccgg atcaccaaat ttatctgttc
     8461 cgtcccaggc aaactcagtg atattcgttc ctatatgcaa tgggccaagc tcaggagcca
     8521 gtacttcgcg gatcactttt ccgctcacgg tcaggatctg aactttaatg taatcaggta
     8581 cttcactgcc agtcagcgtg aacacaaatc tggtttgtgt agtaaatgga ttcgggtagg
     8641 tcagaacatt gctcaccatc ggcttattga ccacttcaaa tccgatgcga tagtccaccc
     8701 cgtcgccact gctgttacca ctcacgtcag ccccgttcac ccataattcg tataaaccat
     8761 cctgactgaa atcaggattc atttcaatcc tggcagtatt gttcgtggca gcggtctcag
     8821 ccggatagaa tatcagggtg ccgtcatggt aagcaaatgg aacaacagat ccgtcaggat
     8881 acttcacaga aacctgcatc agactggtat cagaaagtgc cagaaatgta ttttcatctt
     8941 tcagggcgat aacgatagcc gggctggctg aaacaatatc gccatccaga atatgtactc
     9001 cgtcaaaagt gacgtctatg ttcggatcgg acagatcacc agatacgtgc aggggtaaat
     9061 agcctatgtt gttgaagtga tattgttccg gctggtcaaa cgggggattg acctccacga
     9121 tcagcgtgga agcacctgga ggaatgtctt ttgtgtcaat ggtaatatcg gccgttaacc
     9181 tttcaaatgc acgaatggaa tcctgtcttt caaaaggtac gggatgcaat acattggcag
     9241 catcctgaat ggtgaacgca ataagcatac tatccagatc ccaggcgctc acattatcta
     9301 cagccactct gaaatgcagc ggctcaccct gctgtatgct gtctttctgc caggaaaaat
     9361 cagcaaatgc attcagggca gcctctccta ccggtgtgta gatgacctgc cattcgttga
     9421 gttgagcagg tgttcttacc acatcatcaa aggtattggt gcgcaggcgt atataaggat
     9481 acatgttagc atcgatccag ctgatggtgg tatcgccgga aacaagcccg ctggaaagta
     9541 atgtttgcat tccgtattca tcttctccca gaacctccag atacacactg tctgcttctg
     9601 aatccggagc ttcataattc catttcacct gatcccaggc ggcggcaggt ccaatgcgtt
     9661 ctgaagtgta gctgccttca tcccaaaggc ctgaaatatt gaccacggca tcgatcttgt
     9721 ttcccagcgt gtcgccaatg atctcaaatg cagaagcagg ttcacctttt tttgcataga
     9781 agatgtatga tctgtcaaaa tcatatacag ccagatcgtt gatcatgacc gcaccgtact
     9841 cgttgaatgc atcgaaaagg ctttcgccac ccagtgacaa ggtatcatcg agccactcat
     9901 tgaattgcgg atagttattg ctgtaacaca agaaataggt gccgtctggt acactgtcct
     9961 tcatgaaatg atagaaagca gttctgctct ccagatcagc cgtactgaac tgaatgatct
    10021 gttcataggc atcggcagaa cagtaaatat ctccataagg tccgaaaccg gaatcatcca
    10081 cttcagatac ctgccaggga tagccactta ccggatcgat cacaaatact acaaacccat
    10141 tattggcaca ggagcctaca gccatctctt cgccattcag atagctggtc acttcggtcc
    10201 agtcagtggt agggtaaata cctgtagcca cctggtagtt gacatagttg ttgacgaagt
    10261 ggaaattgcg gtcctcatcc agagagatgt tatcgtataa atcatcggca tactgaaaat
    10321 aatggctttg attccaacct atctcctcgc cgcttttata acggaaggaa cttgtgcgcc
    10381 agagataatc agcaccacct gcagtatcca cactgacacg ccagtaatat accctgccgg
    10441 agagccatgt aacgggcggc gcttcccatt tcagcactcc tccgctctgg gtaacagaat
    10501 ggctttgcaa caaaggactg ttaaaagagg ctgtggtatc gatctgcatc acataagtac
    10561 gggtacctgc aaatggatta cttgtggaag cagcaagata atcaggtgct tcattgatca
    10621 gtgcaaattc atagggatag accgggttag catcatcacc aagtatcagc tgggtcttgc
    10681 taataacatt attccactct gcgatctcag ctatggaatc tccatagtca acggtaatgg
    10741 taaaactatt caatcccact ccgctgttag catcggtaaa aagcctgata cctacggtat
    10801 cccgaaactg agcacttctc agtgttcggg taaccagctc actgctgcca tcaggcctga
    10861 tcctttcaac tgatactgtg taatccttgt caatggccat tccaatgttg gtcaccacca
    10921 cctgcactgt aaaagagtct tccagtgtgg taataactga aggttcaaaa aacacatagg
    10981 gttcttctat cacataatcc ggtaaactat gggtattgag cctgagagcc ggatcaccct
    11041 gcagggctgt atgctgcagg gtcaggaggt cataaatgtt gtcaaaagcg gaaatatatt
    11101 ctgctgcacc ctgcagtgca tctcctatgc cctgaccata cctgtatcgg gaaagttctg
    11161 catagaatct caggccatag ttatacaggc tgcctgcaag tgaaaaagta gtggcagcaa
    11221 taaatgcgat agaggccctg tcttcggtat tgatatactt ttcacccagt gtgatggtgc
    11281 catcaaagat tgccgcagta ttgcatccgt taccaaaaat cacattgtac tttccataat
    11341 tcgtaaaatc ttcaggatcg ccgatattgt aatcaaatga gccggtagtg ctgtgaccaa
    11401 agaatgtgac cagtgaaact ccactattga taagggaatc gatatagtct gatgtttcat
    11461 aaaaaattgg atctgtagtg atcttgttga actgtataac attggctcca tacagcgtgt
    11521 cttcagcaat atcacccagg ttacgcaggt aggtattgaa caggctctgc tcatattcat
    11581 tcaaccctcc tgcaaaatgc agcatgttct tcatccatgc tttgctttca atagtctgat
    11641 cggtcgttgc ctgcacagtt tcgtactcta ccactttatc caggtagttt ttcacttctg
    11701 tgccgttata tgctaccagt ctgccaactg gaagttccgg aatatagctt cccggcctgc
    11761 acaccaggag tacatctgaa cccggattgc caaatgtagg tacggtattc tgcgcaaagc
    11821 tttctgcatt gttcctgcaa ccatcatact ttcttgcttt gcctatcagg aacagatacc
    11881 ttggatcggt ttcaaaatga tcttcggcaa agaatacaaa attcctgaca gccagcggat
    11941 gtgttttaat accataagca aactgatcgg tcagctgtgc tatatccgca atcagcggct
    12001 ggaaagatcc gcctgcaaac ccctctctat agtcagcata agcttgtatc cagtcttcac
    12061 cagttccgtc atcacgcaac cctgcatgtg aaatgatcag gtagtcaccc tggttgaccg
    12121 ggttactgaa atcaatgaag gttaccggct ccagttcctg cacctgcaga atatcaaggg
    12181 tatcctggct gctcagccat atcctgttgc ctgaagcatc gtaaggcata tggatctctg
    12241 tattggaacc aacaatggta ccgatcattc ttttgtgatc gtcaaaatcc cagataaccg
    12301 gatcggtgga cttttcattg aaatcagtga tgaccagata tttggtggcc gataaagagt
    12361 tgaccagccg cagacccact ttgctggcat tattgaaatc ccagatacgc ggataggtga
    12421 tctcaatata acagtttcgc agatagtcat tactgccatt cagtgacgca aacgacacgg
    12481 tattgctgct caacaaattg tctatagaaa attcatattt tttaaaatca tatcctgtaa
    12541 aactggtgtc atacaaagtg gttccattaa aggaaatggt atagtgatgt tccacttcat
    12601 tggttgcaat gaccgctgtt cgaagggtgg gcacaaaagc atcttctccg gagtatatat
    12661 tggaagtgct cagtgtcttg tttctggtgg tacctgcaaa cctggtctca gaaaaacctt
    12721 ctccgtcgtc atatttagat gaatagatct ccaggtaata ccttccggga gcaaaataac
    12781 ttgcaccgtg attcaacacc gtagtggagc cgttgatcat gagggtgcga aattcataat
    12841 gcgattccgg ttctggcatg tcactaagca cattggccaa ttgttccatg cgcaggtggc
    12901 ccggatctga gttccaggtg agaaaataaa ctgcggtatc ggtaaagagg ctggtatagg
    12961 tatgtatctg atactgctca ttttcataga gacgggtatc cagctttcca tcattacgga
    13021 ttccgtaaaa ctcaatgaag tctcccggat caaagctgcc atctccatct ccttcaatgt
    13081 acagatattg ctcttcgccc agctgaaata gctgaaagtc gtcaggagca atagtggcta
    13141 tatctgtacc gatggctgta agtgcgtctg ccagctcatc atagtgcagg cggtaaagcc
    13201 cgtctgcagc tgttctgatc ttcagatact gctgatcata tgatatccat tcattgccat
    13261 acaggtctgt ttgcgccgac agtgccgaag cgaaccagag cagtacaata agcgagggta
    13321 tgcgtttgat cataggcgca ggttagcaga tcagttttga tcaggaacct gacgcgaagg
    13381 catggacaac tgcagcgaca ccacatgaga atacagcact tccgcctggt ttcctatatt
    13441 agtataagcg taatcaagat gtataggtcc tatgaccacc cccacgccca gattgggttg
    13501 caatacccag ctttcagtgg tagcgtcatc tgcaatggcc tgttgcacat tattcactcc
    13561 ggccctcaga taaatgaaat ctttatagcc ggcttcaatg cccagatg
//
LOCUS       DAOMAX010000051        13607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_544_length_13607_cov_4.564419, whole genome shotgun sequence.
ACCESSION   DAOMAX010000051 DAOMAX010000000
VERSION     DAOMAX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_544_length_13607_cov_4.564419"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1100
                     /gene="gap"
                     /locus_tag="PK742_04415"
     CDS             90..1100
                     /gene="gap"
                     /locus_tag="PK742_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQU75866.1"
                     /translation="MNTIKIAINGFGRIGRLVFRQIHDLDGMEVVAINDLTNPQTLAH
                     LLKYDSAQGRFEQEVKATEDSIIVNGNAIRIYAERNPADIPWDKHEVDVVIESTGFFT
                     SEEKAHAHITAGAKRVVISAPATGDMKTVVFNVNHDILDGSETIISCASCTTNCLAPM
                     AQVLHKNFGIAIGSMTTVHSYTNDQNTLDAPHSKGDLRRARAAAANIVPNSTGAAKAI
                     GLVLPELKGKLDGGAQRVPTITGSLTELTCVLGMTVTKEEIHAAMKAASNETLGYNED
                     PVVSSDIIGMTYGSLFDATQTKVISVGDKQLVKTVAWYDNEMSYVSQLVRTVRYFAEL
                     IA"
     gene            1125..2318
                     /locus_tag="PK742_04420"
     CDS             1125..2318
                     /locus_tag="PK742_04420"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQU75867.1"
                     /translation="MNTITDFDFNGHKALIRVDFNVPLDKETQAVTDDTRLRAALPTI
                     NKILGDGGSVILMSHLGRPKDGPEEKYSMKHVIGRLESLLGRPVLFATDCIGESAASA
                     ASKLQPGEVLLLENLRFHPEETKGDEDFAEQLSRLGDVYVNDAFGTAHRAHASTTIVA
                     KFFDAGHRMAGLLLAAEVEAGTKVLRNAEKPFTAIIGGAKVSDKILILEKLLDLADNI
                     IIGGGMAYTFYKAQGGHIGSSLCEEDRLDAALQLLEQASAKGVNVYLPEDSILADRFA
                     ADAATQVTASKEIPDGWMGLDIGPAALREFSYVIAQSKTILWNGPMGVFEMEAFQKGT
                     AEVAQAIARASAQGAYSLVGGGDSVAAINTFHLSDKVSYVSTGGGAMLEFFEGKELPG
                     VAALN"
     gene            2603..3058
                     /gene="mraZ"
                     /locus_tag="PK742_04425"
     CDS             2603..3058
                     /gene="mraZ"
                     /locus_tag="PK742_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQU75868.1"
                     /translation="MSGLLGEFEVSMDPKGRVKVPSALIKQLSADDNARFVITRGFEK
                     CLVLYPYSEWQKVSARLNSLNMFVKKNRDFVRYFMSGATELTLDASERVLLPKPLQDY
                     ANITKELVLSAFQGKVEVWDKKQYHSTIDMNSDDFAGLAEEVMGNTPDE"
     gene            3051..3947
                     /gene="rsmH"
                     /locus_tag="PK742_04430"
     CDS             3051..3947
                     /gene="rsmH"
                     /locus_tag="PK742_04430"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQU75869.1"
                     /translation="MSEAYHIPVLLHESVSALNIIPDGIYVDVTFGGGGHSRAILNEL
                     GPLGKLIAFDQDPEAAENAPNDDRFLLIPWNYRYLKRMLRVAGIDQVNGILADLGVSS
                     HQFDEASRGFSYRFDAPLDMRMNTEAGTTAAGILAQWDEAALQDMFSKNAELRNARQL
                     ARAITMTRVQSPLLTTGDLVRLAETVMKGERHKYLAQVFQALRIAVNDELNGLQDMLQ
                     DAAEILQPGGRLVVISYHSLEDRIVKDALRPHEDDNPANMTGQKEEHFRIIARKPVVP
                     EAEEIKNNPRAGSARMRIAERQ"
     gene            3964..4314
                     /locus_tag="PK742_04435"
     CDS             3964..4314
                     /locus_tag="PK742_04435"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HQU75870.1"
                     /translation="MTRQTDSKRKKNVSLAALLRNLEKVPFLQTDYIASSIPFFIFLL
                     MLGFFYIWNNHRGVTMVKDLRNTEQQMTEAQWEFNDAKNELTRYSRQSTVAERVRDQQ
                     MYELTDPPYTIIDK"
     gene            4311..6407
                     /locus_tag="PK742_04440"
     CDS             4311..6407
                     /locus_tag="PK742_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HQU75871.1"
                     /translation="MTEVKKDILWRLYLTYAFFVLAGVAIVVQILRIQLVEGEDLRAD
                     ASEKQIKSVSIAAKRGSIYAEDGSLLAASFPYYRVIMDPSVIDAKVFARDLDSLCLGL
                     SRVLKDQPPSYYKEKILQARRTESKYLVLHKKATVPQYYELQKLPVFRLGRNRGGLYK
                     EEIQVRERPYGSLASRTVGIERENNKVGLEGAYDSVLAGVTGLQLQRRVAGNVWMPLN
                     DANQVDPVDGLDIITTIDVNIQDITETALRRILIKNDAAWGTAVVMEVATGKIRAISN
                     LTRLEEGNYAEVLNYAVGTKVEPGSTFKLFSLMALLEDGYVTIQDSVDLNLGTYQYGD
                     RVMKDSEGKHEYRNVTVEKAFANSSNVGISRLVTEHYRTNKEKYVKHITDIGLDKPTG
                     VCIPGEPAPKFKLDPSDKKNWSGVTLPWMSVGYELEITPLQMLTFYNAVANDGKMMQP
                     MLVEGTSRYGQMQETYKPVVLNQKICSDKTLKQVQACLEMVVDSGTAKNIRNDHYKIA
                     GKTGTAQRLVNRFYSNTSYLASFAGYFPADDPKYSCIVVVSEPSKLVFYGGYVAGPVF
                     REIADKVYSHDLRMVGPVNASDSSYAGLTTRTKGYAGDFREILDWMDISEPAGNSSEW
                     WMLSIDADKPKVSDVAIMDKVMPSVKGMGLRDAMYLLETMGLKVEVSGTGKVVYQSLS
                     PGTGIYRGAKVFLKLG"
     gene            6404..7870
                     /locus_tag="PK742_04445"
     CDS             6404..7870
                     /locus_tag="PK742_04445"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQU75872.1"
                     /translation="MKTLQQLITDLDVRSLRGRVDATVHAVTADSRQVAYGTLFIAVK
                     GTAHDGHAFIPQVVKAGAEVIVCERIPEAADTSVTWVEVANSSQALGVIAHTFFDHPT
                     RELKLVGVTGTNGKTTTATLCYQLFTALGYPTGLLSTIENRILKEVLPATHTTPDPVQ
                     LNRILRQMVDAGCSHAFMEVSSHAAHQHRIAGLEFSGAVFTNITHDHLDYHGTFDNYI
                     AAKKMFFDALPASAFALVNADDKRASVMLQNTKADTRRFSVKSDGDFTCRIIANEITG
                     LQLKLDGEEFHSRLTGEFNAWNLLAVYSVARLLGLSKQEVLPALSALGKVNGRFEVLR
                     AAEQNLTAVVDYAHTPDAVEKVLETLRGMLHKQQGIITVIGCGGDRDKTKRPLMAAAA
                     AKWSTQVVLTSDNPRTEDPALIIRDMENGLDEEMRRHTLSIADRKEAIRTAIKLSRPG
                     DVVLVAGKGHETYQEIMGVKHPFDDKQVITETFQNLNR"
     gene            7870..9174
                     /gene="mraY"
                     /locus_tag="PK742_04450"
     CDS             7870..9174
                     /gene="mraY"
                     /locus_tag="PK742_04450"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQU75873.1"
                     /translation="MLYYLFNWLEAEFNIPGAGLFQFLSFRAAMAILFSLIISIIFGK
                     RIIRYLQKQQIGESIRDLGLEGQLSKKGTPTMGGIIILISLIIPTLLFADLGNIYIIL
                     MLIVTVWMGIIGFLDDYIKVFRKNKEGLRAKFKLLGQIVLGLIVGLVLYFSDDVKIRD
                     YEHIYVYEVENGQPVGKPVESYDANEVLLEGNGMLNADGTRYLSHDTRSSITTIPFVK
                     NNEFDYEWLVAWAPKNARGWVFPVIYILIIITIITAVSNGANLTDGLDGLAAGTSSII
                     VFTLLIFSYVAGNFLFADYLNIMYIPNSGELVVFSAALIGACVGFLWYNAFPAQVFMG
                     DTGSLAIGGIIASLAIIIRKELLIPILCGIFLMENLSVMMQVSYFKYTKRKYGEGRRI
                     FRMSPLHHHYQKSGMHETKIVARFWIVGVFLAIITIVTLKVR"
     gene            9178..10515
                     /gene="murD"
                     /locus_tag="PK742_04455"
     CDS             9178..10515
                     /gene="murD"
                     /locus_tag="PK742_04455"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQU75874.1"
                     /translation="MKQRMVILGAGESGTGAARLAVRKGYDVFVSDKGKIAEKYRMML
                     QAEGIAFEEGRHSMERILAADEVIKSPGIPESVPVLRAIREKGIPVVGELEFGFRHCH
                     GKIIAITGSNGKSTTTKMTWWILHRAGLDVAMGGNIGKSFAGILADGDHDWYVLEVSS
                     FMLDDTATFRPFIAMLLNITPDHLDRYAYQMENYVASKFRITANQNESDLFIYNADDP
                     VIREGLKRHPSRAVPLTFTAADAPRQGAWISGSKIQFQYNQNTFDMTISELAVQGKHN
                     RYNSMAAAISGHVLDIRKESIRESLMDFTGLEHRLEFVAKVHGISFINDSKATNVNST
                     WYALESMNDPVIWIAGGVDKGNDYSEVLDLVKKKVKAIVCLGTDNRKLQEAFSRHVDI
                     MVTTDTMQEAVDMAYRLGKDGDAVLLSPACASFDLFENYEDRGNQFKNIVREL"
     gene            10520..11707
                     /locus_tag="PK742_04460"
     CDS             10520..11707
                     /locus_tag="PK742_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQU75875.1"
                     /translation="MTAVQFLKNKLRGDRYIWLVVLGLSLFSILAVYSSTIALSFQEA
                     NGNTEYFVLKHIGIMLFGVVMMYLAHLIPYRFYSRISQILLWLSIPLLIYTLFFGVEI
                     NDASRWITIPVVNLTFQTSDLARFALIMYTARLLSRKQELVHSFKEAFVPVMLPVGLV
                     CALIFPDNLSTASILFVTCLLLMFIGRVHFKYIAVTALSGLLLVASIIALSYLMPDVG
                     RFATWQSRLESFTGGGDVPDQVVVAKTSIAMGGVLGQGPGNSTQVHHFPLAYADYIYA
                     LIIEEYGLLGGVVILLLFLILMYRCIRIVVYAPSSFGALLAIGLGLSLTIQALINMGV
                     ATSLLPVTGLTLPFISMGGTSLWFTSLSFGIILSVSREIELLKLQEQGELPEDETIHE
                     GIE"
     gene            11679..12803
                     /gene="murG"
                     /locus_tag="PK742_04465"
     CDS             11679..12803
                     /gene="murG"
                     /locus_tag="PK742_04465"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQU75876.1"
                     /translation="MKQFMKESNKTYKLLVSGGGTGGHIFPAVAIANAFRRRHPETEV
                     LFVGAKGKMEMQKVPAAGYPIEGLWISGFSRSLSWTNLLFPVKLIHSYFRAGAIVRRF
                     RPDLAVGTGGFASGPALNAAMRAGIPAIVQEQNSFPGITNKILSRKARKVCVAYEDME
                     RFFPAGKTELTGNPVRREILDNQATREEGRAHFGISTRKKVVLVVGGSLGSRTFNECM
                     MTDLNRIRQEEIHVIWQTGTLMFDQCRKAASGMDNVSVVDFIPEMAKAYAAADVIVSR
                     AGAIAISELCVVGKPVILVPFPFAAEDHQTKNAQALVDKGAAVMISDADAPARLVDAM
                     LHLLRDPEKQEHLSAAISQLAVTNAAERIADIMENILGIA"
     gene            12804..>13607
                     /locus_tag="PK742_04470"
     CDS             12804..>13607
                     /locus_tag="PK742_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HQU75877.1"
                     /translation="MEQLSRIYFIGIGGIGMSALARYFHGKGLFVSGYDRTETELTRN
                     LQQEGMTICFEDDVALLDKDADLVVYTPAIPADHQQLNYYRDHGYQLKKRSQVLGELT
                     HDHFTIAVAGSHGKTTTSSMIAWILRDSGYDCTAFLGGICVNFESNFVQGANQVIVVE
                     ADEFDRSFHTLHPDIAVVTAIDSDHLEIYGTQEALEENFLVFAKQIHDNGLLIHKQQV
                     TALQAHTGHRSTYSLRDDAADLYAVSTEIGEQGSRVILSNGFDFVLEYPG"
BASE COUNT         3630 a         3293 c         3533 g         3151 t
ORIGIN      
        1 agccgatttc cggttttttc taaacaaatc gggaaaaaaa atcgttggcc gggtatcatc
       61 ctaattatca caaaactatt tttgcggcca tgaatacaat aaagatcgcc atcaatggat
      121 tcggacgtat cggaagactg gtcttccgcc agatccacga cctggatggc atggaggtag
      181 ttgctattaa cgacctcacc aatccccaga cccttgcaca tctcctgaag tatgacagcg
      241 cccagggcag atttgaacag gaagtaaaag ctacagaaga cagcatcata gtgaatggca
      301 atgccatccg catctatgct gagcgaaatc cggcagatat tccatgggat aaacacgaag
      361 tcgatgtggt cattgaatca accggtttct tcaccagtga agagaaagca catgcccata
      421 ttaccgctgg cgccaaaaga gtggtcattt cggctccagc aaccggcgac atgaaaacag
      481 tggttttcaa tgtgaaccat gatatcctgg acggatcaga gaccatcatc tcctgcgctt
      541 cctgtaccac caactgcctg gctcccatgg cacaggtact gcataagaat ttcggcatcg
      601 ccatcggcag catgaccacg gtgcactcct ataccaacga ccagaacacc ctcgatgcac
      661 cgcatagcaa aggtgatctg cgtcgtgcaa gagctgcagc agccaatatc gtacccaact
      721 cgacaggagc agccaaggcc atcggcttgg tgctgccgga actgaaaggt aaactggatg
      781 gaggtgcaca gagggttccc accataacag gttctcttac tgaacttacc tgtgtgctgg
      841 gcatgaccgt gactaaagaa gagatacatg ctgccatgaa ggcggcttcc aatgagactt
      901 tgggatacaa cgaagacccc gttgtcagct cagatatcat cggtatgacg tacgggtccc
      961 tgtttgatgc tacccagacc aaggtcatca gcgtgggcga caagcagctg gtcaagaccg
     1021 tggcatggta tgataatgaa atgagttacg tgagccagct cgtgcggact gtgcgctatt
     1081 tcgctgaact gatcgcctga caagggcctg agaaaactac tactatgaac accattactg
     1141 attttgattt caatggacat aaagccctga tccgggtcga tttcaacgta ccgctggaca
     1201 aggagaccca ggctgtgacc gatgatacca gattaagagc agctcttcct accatcaata
     1261 agatccttgg cgatggcgga tccgtcattc tgatgtcgca tctgggcaga ccgaaagacg
     1321 gtccggaaga gaagtacagt atgaagcatg tgatcggaag gctggaatcg cttctgggca
     1381 gaccggttct tttcgccact gattgcatcg gtgagtcagc tgcatcagca gcttcgaaac
     1441 tgcaaccggg tgaggtgctg ctgctggaaa atcttcgctt tcatccggaa gagaccaaag
     1501 gagatgagga ctttgcagaa cagctttccc gtcttggaga cgtttatgtg aatgatgcct
     1561 ttggcactgc tcaccgggca cacgcctcca ctaccatcgt agcaaagttc ttcgacgcag
     1621 gtcatcgaat ggccggcctg ttgctggcag cggaagtgga agccggaacg aaagtgttgc
     1681 gaaatgctga aaaacccttc accgcgatca taggcggtgc caaggtgagc gataagatcc
     1741 tcattctgga gaaactgctg gacctggccg ataacatcat tattggcgga ggaatggcct
     1801 atacttttta taaagcacaa ggtggccaca tcggcagctc gctgtgcgaa gaagaccgac
     1861 tggatgctgc tcttcagctt ctggaacagg cttcagcaaa aggggtgaac gtgtacctgc
     1921 cggaagacag tatcctggcc gacaggttcg ctgcagatgc tgccacgcag gtaaccgcca
     1981 gcaaggagat ccctgacgga tggatgggcc tcgacatcgg accagccgcc ctgcgggaat
     2041 tcagctatgt gatcgctcaa agcaagacca tcctctggaa tggcccgatg ggtgttttcg
     2101 aaatggaagc atttcagaaa ggtactgcag aagtagccca ggccattgcc cgtgcgtctg
     2161 ctcagggtgc atactctttg gtaggtggcg gcgattctgt agcggctatc aataccttcc
     2221 atctgtctga taaggtcagt tacgtaagca caggtggcgg cgccatgctg gagttctttg
     2281 aaggaaaaga gcttcccggg gtagcagccc tgaactgata attcatatca ttagtaatac
     2341 cacctgcggt ggtcagcatg gctgaccacc gctttttttt gcacctaagc ttccgtttct
     2401 tctcgcttgc ccccactgtt tgcggcactc tgtgacaggg tgatctgcac ctctaggacc
     2461 cgtttttagc tgtcatccac aattgtgcgt aaccagtgtg cattgtttgt gaataaaaat
     2521 aggtgggtta aagtgggaga aagtgggaga aaatggaata tttgcgtcag ttttacgata
     2581 catttccacc agaacaggaa ttatgtcggg tttattaggc gaatttgaag taagcatgga
     2641 tcccaaaggg agggtcaagg tgccgtcagc gctgatcaag cagctgagcg ccgatgacaa
     2701 tgcacgcttc gtcatcaccc gaggtttcga gaaatgcctg gtactgtatc cctactctga
     2761 atggcaaaag gtgagtgccc gtctgaacag cctcaacatg ttcgtgaaga agaaccgcga
     2821 cttcgtgcgc tacttcatga gcggtgctac cgagctcaca ctggatgcca gcgaaagggt
     2881 tctcctgcct aaaccccttc aggactacgc caacatcacc aaagaactgg tgctgtcggc
     2941 cttccaggga aaggtggagg tatgggataa gaaacagtac cacagcacca tcgacatgaa
     3001 ctccgacgat ttcgcaggac tggcggaaga agtgatgggt aatactcccg atgagtgagg
     3061 cctaccacat accagttctt ttacatgaaa gcgtaagcgc acttaacatc atccctgacg
     3121 gtatctatgt agatgtcacc ttcggtggtg gcggtcatag ccgggctatc ctgaatgaac
     3181 tcgggccact tggaaaactg atcgcttttg accaggatcc ggaagcagct gagaatgcac
     3241 caaacgatga tcgtttcctg ctcatcccct ggaactaccg ttatctgaag cgcatgttgc
     3301 gggttgctgg catcgatcag gtgaacggca tactggcaga tctgggagtg tcttcccacc
     3361 agttcgatga agcctccaga ggattctctt accgcttcga tgctccgctc gatatgcgga
     3421 tgaacacaga agcaggaaca actgccgcag gtatcctggc gcaatgggat gaagcagctc
     3481 tccaggacat gttcagcaag aacgcagaac tgcgaaatgc cagacagctg gccagagcga
     3541 tcaccatgac cagggtgcaa tcgccgctgc tgaccaccgg tgatcttgtc aggctggccg
     3601 agaccgtgat gaaaggcgaa aggcacaaat acctggcaca ggtgtttcag gcactccgca
     3661 tcgcagtcaa tgatgagctc aacggtttgc aggacatgct gcaggatgct gcagagatcc
     3721 ttcagcccgg cggaaggttg gtggtcatca gctatcactc tctcgaagac agaattgtga
     3781 aagacgcatt acgcccccat gaagatgaca atcccgccaa tatgacgggg caaaaagaag
     3841 agcacttccg catcatcgcc cgaaagccgg tggtgcctga ggcagaagag atcaaaaaca
     3901 atccgcgagc aggaagtgca cggatgagaa tagctgaaag gcaataaaaa acgaaagacc
     3961 aatatgacca ggcagacaga cagcaaacga aaaaagaacg tgtcgctggc tgcgcttctc
     4021 cgcaacctgg aaaaggtgcc ctttctccag acagactaca tcgccagcag tattccattc
     4081 ttcatttttc tgctcatgct gggattcttc tacatctgga acaatcatcg cggcgtgacc
     4141 atggtgaaag acctgcgcaa taccgagcaa caaatgaccg aagcgcaatg ggaattcaac
     4201 gatgccaaga atgaactgac cagatacagc aggcaaagca ctgttgcaga gcgggtcaga
     4261 gaccagcaga tgtatgaact gaccgatcca ccttatacca tcatagacaa atgacggaag
     4321 tcaaaaaaga tattctctgg aggttatacc tcacctacgc cttctttgtg ctggcggggg
     4381 tagccatcgt cgtgcagatt ctccgcattc aactggtgga aggagaagat ctccgtgctg
     4441 atgcaagtga aaaacagatc aagtctgtat ccatcgctgc caaacgcgga agcatctacg
     4501 cagaagacgg ctccctgctg gcggcctcct ttccatacta ccgcgtcatc atggatccat
     4561 cagtgataga cgccaaagta tttgccagag atcttgactc gttgtgcctt ggtttaagcc
     4621 gggtgttgaa agatcagccg ccatcttatt ataaggaaaa gatcctgcag gcacgacgca
     4681 cggaatccaa gtacctggtg ctgcataaaa aggcaaccgt tcctcaatac tatgaattgc
     4741 aaaaactgcc cgtttttcgc ctgggcagaa ataggggagg tctctacaaa gaagagatcc
     4801 aggtaagaga aagaccctat ggcagcctgg cttcgagaac tgttggtatt gaacgggaaa
     4861 ataataaagt tggcctggaa ggagcctatg actctgtgct ggcaggggtt accggattgc
     4921 agttacaacg cagggttgca ggcaatgtat ggatgccgct caacgatgcc aaccaggttg
     4981 atccggttga cgggctcgat atcattacaa ctattgatgt aaacatccag gatattacag
     5041 aaacagcctt gcgtcgtatt ctcatcaaga acgatgctgc ctggggaacc gctgtagtaa
     5101 tggaagtagc taccggtaag atccgggcca tcagtaacct gaccagactg gaagaaggca
     5161 attatgccga agtactcaac tatgcagtgg gcaccaaagt ggaacccggt tccaccttta
     5221 agcttttttc tctgatggcc ttactggaag atggttacgt gaccattcag gacagtgttg
     5281 acctcaatct gggcacttat cagtatggcg acagggtcat gaaagacagt gaaggcaagc
     5341 atgaataccg caatgtgacg gttgaaaaag cctttgccaa ttcctccaat gtgggcatca
     5401 gcagactggt cactgaacac taccgcacca ataaggagaa gtatgttaag catatcacag
     5461 atatcgggct ggataaacca accggtgtct gtattccggg tgaacccgca ccgaaattca
     5521 agctggatcc gtcagataaa aagaactggt ctggtgttac tctcccttgg atgagcgtgg
     5581 gctatgaact ggagatcacc cctctgcaaa tgctcacctt ttacaatgca gtggccaacg
     5641 acggaaagat gatgcagccc atgttggtgg aaggcacatc ccgctacggg caaatgcagg
     5701 aaacctataa gcctgtagtg cttaatcaga aaatatgtag cgacaaaacc ttaaagcaag
     5761 tacaggcatg tctggaaatg gtggttgaca gtggtacagc aaaaaatata cgaaatgatc
     5821 attataaaat agccggaaaa accggtacag cacaacgttt ggtgaaccgt ttctacagca
     5881 acacttctta cctggccagc tttgcaggat acttcccggc tgatgaccca aagtattcct
     5941 gcattgtagt ggtaagtgag ccgtccaagc ttgtattcta cggtggttat gtagcaggcc
     6001 cggtattccg cgagatcgca gacaaggttt attcgcatga tctgcgcatg gtaggaccgg
     6061 tcaatgccag cgattcttcc tacgccggac taaccaccag gaccaaaggt tatgcaggtg
     6121 atttccgtga gatactggat tggatggaca tcagcgaacc ggctggaaac agcagtgaat
     6181 ggtggatgct ttcaatagat gcagataaac ctaaagtgtc tgatgtggcc atcatggata
     6241 aggtcatgcc atctgtcaag ggtatggggc ttcgcgatgc gatgtacctg ctagaaacaa
     6301 tgggtctgaa ggtagaagtg agcggaacgg gcaaggtggt atatcagtca ttgtcacctg
     6361 gtaccggaat ctaccgtggt gcaaaagtat ttctgaaact gggatgaaaa cactgcagca
     6421 actcataaca gatctggatg tgcgttccct gcggggaagg gtggatgcaa cagtgcatgc
     6481 cgttacggcc gatagccggc aagtagcata tggaaccctg ttcatcgcag tgaaaggtac
     6541 cgcacatgat ggtcatgctt tcattcctca ggtagtgaaa gcaggcgccg aagtgatcgt
     6601 atgtgaaaga atacccgaag cagcagatac atctgtaacc tgggtcgaag tggctaacag
     6661 cagccaggca ctgggggtga tagcccacac attcttcgac catcctacac gtgaactgaa
     6721 actggtggga gttaccggta ccaacggcaa gaccaccact gcaacattgt gctatcaact
     6781 gttcaccgca ctgggttatc ctacgggatt gctctcaacc attgagaacc gcattctgaa
     6841 ggaagtactg ccggctactc ataccacacc tgatccggtg caactcaatc gcatcctacg
     6901 gcagatggtt gatgccggat gcagccatgc cttcatggag gtgagttctc atgccgcaca
     6961 tcagcaccgt atagcgggac tggaatttag cggagctgtt ttcaccaata tcacccatga
     7021 tcacctggac taccacggca catttgataa ttatatcgcc gcaaagaaaa tgttctttga
     7081 tgctttacct gcatctgcct ttgcactggt aaatgcagat gacaaaaggg catcagtgat
     7141 gctgcagaac acaaaggcag acacccgccg cttctcagtt aagagcgatg gtgatttcac
     7201 ttgccgcatc attgccaatg agatcacagg tctgcaactg aaactcgatg gcgaagaatt
     7261 tcattccaga ctgaccggcg aattcaatgc ctggaatctg ctggcggttt atagcgtggc
     7321 acgtttgctc ggcctcagca aacaggaagt tttacctgca ctcagcgcac tgggtaaagt
     7381 aaatggtcgc tttgaagtat tgcgtgccgc tgagcagaac ctgaccgctg tggttgacta
     7441 tgcacacaca ccggatgccg tagagaaagt gctggaaaca cttcgtggca tgctgcacaa
     7501 acaacagggc attataacgg taataggttg tggtggagat cgcgacaaga ccaaacgccc
     7561 gcttatggct gcagcagcag caaagtggag tacacaggta gtgctcacgt cagacaatcc
     7621 ccgcacggaa gatcccgcac tgatcatccg cgacatggag aatggactgg atgaagagat
     7681 gcgcaggcat accctgtcta tcgccgacag aaaagaagcc atccgcactg ccatcaaact
     7741 gtccagaccc ggcgatgtgg tgctggtggc cggtaaaggg catgagacct atcaggaaat
     7801 aatgggtgtg aaacaccctt tcgacgataa gcaagtgatc acggaaacct ttcaaaacct
     7861 gaaccgctaa tgctgtacta tctgttcaac tggctggaag ctgaattcaa catacccgga
     7921 gccggactat ttcagttcct ttcattccgg gcggccatgg ccatactgtt ctccctgatc
     7981 atctcgatca ttttcggcaa acgcatcatt cgctacctgc agaaacaaca gataggcgag
     8041 agcatccgtg accttggcct ggaaggacaa ctgagtaaaa aaggtacacc tactatggga
     8101 ggtatcatca tcctgatcag cctcatcatt cccacattgc tgttcgcaga tctgggtaac
     8161 atctacatca ttcttatgct cattgtaact gtttggatgg gcatcatcgg tttcctggat
     8221 gactacatca aggtgttccg caagaacaag gaaggtcttc gtgcaaagtt caaactgctt
     8281 gggcagatag tgctgggttt gattgtaggt ctggtgctgt atttcagtga tgatgtcaag
     8341 atcagagatt atgagcacat ctatgtgtat gaagtggaga acggccagcc tgttggaaaa
     8401 ccggtggaat cgtatgatgc caacgaggtg ttgctggaag gtaacggtat gctgaatgcc
     8461 gatggcacca ggtacctgtc gcacgatacc cgttccagta tcaccaccat ccctttcgtc
     8521 aagaacaatg agtttgacta tgaatggctg gtggcatggg cgccgaagaa tgcacgtgga
     8581 tgggttttcc ctgtcattta tatactcatc atcatcacca taatcacggc cgtttcgaat
     8641 ggggccaacc tgaccgatgg tctggacggt cttgcagcgg gcacctcgtc gatcatagtt
     8701 tttacattac tgatattttc atacgtagcc ggtaactttc tgtttgcaga ctacctcaat
     8761 atcatgtaca tcccaaactc aggtgagctg gtggtattct ctgctgcatt gatcggagcc
     8821 tgtgtgggct ttctctggta caatgcattc ccggcacagg tgttcatggg cgatacggga
     8881 agtctggcca ttggcggcat catcgcatca ttggccatca tcatcagaaa ggagctgctt
     8941 atccccatcc tctgcggcat ttttctcatg gagaatctca gtgtgatgat gcaggtaagc
     9001 tatttcaagt acaccaaacg aaagtatggc gaaggcaggc gcatcttccg catgtcgccg
     9061 ctccatcacc actatcagaa atcaggcatg cacgagacca agatcgtagc ccgcttctgg
     9121 atcgtgggtg ttttcctggc catcatcacc atcgtaaccc tcaaagtcag ataatccttg
     9181 aagcagcgca tggtcatatt gggagcagga gaaagtggca ccggtgccgc ccgcctcgct
     9241 gtgcgcaaag gatacgatgt attcgtgtct gacaagggaa agatcgcaga aaagtacagg
     9301 atgatgctgc aggcagaagg tattgctttt gaagaaggcc ggcactcaat ggaacgcatt
     9361 cttgcggctg atgaagtgat caaaagcccg ggaataccgg aatccgtacc ggtgctccgg
     9421 gccatcagag aaaagggtat tccggtggta ggtgaactgg aattcggatt ccgtcactgc
     9481 catggaaaga tcatcgccat cactggcagc aatggaaaaa gcactaccac caaaatgacc
     9541 tggtggatac tccatcgtgc cggccttgat gtggccatgg gcggaaatat cggaaagagt
     9601 ttcgccggca ttctggccga tggagaccac gactggtatg tactggaggt cagttcgttc
     9661 atgctggatg ataccgcaac cttcaggcca ttcatcgcca tgctgctgaa catcacaccc
     9721 gaccacctgg atcggtatgc ataccaaatg gaaaactatg ttgcgagcaa attccgcatc
     9781 actgccaacc agaacgaatc agatcttttt atatacaatg cagacgaccc ggtcatcagg
     9841 gaggggctga aacgccatcc ctcccgggct gtacctctca cattcacagc tgccgatgca
     9901 ccccgtcaag gtgcctggat aagcggcagt aaaattcaat ttcaatacaa ccaaaacacc
     9961 tttgatatga cgatcagtga actcgcagta caaggcaaac acaaccgcta caactcaatg
    10021 gcagcagcta tttcagggca tgtgctcgac atccggaagg agagtatccg cgaaagtctg
    10081 atggacttta ccggactcga acaccgcctg gaatttgttg ccaaggtaca tggcatcagc
    10141 ttcatcaatg actccaaagc taccaatgtg aactccacct ggtatgcgct ggaaagcatg
    10201 aatgatcctg ttatctggat agcaggtggc gtagataaag gaaatgacta cagcgaggtg
    10261 ctggatctcg ttaagaagaa agtaaaggcc atcgtctgcc tgggtactga caaccggaaa
    10321 ctccaggaag cattcagccg gcatgtggac attatggtca ccacagacac catgcaggaa
    10381 gctgtagata tggcctaccg cctgggtaaa gacggtgatg ctgtgctgtt gtcgccggca
    10441 tgcgcctcct ttgatctgtt cgaaaattat gaagaccggg gcaaccagtt caagaacatc
    10501 gttagagaac tctgatacta tgacagctgt ccagttcctg aaaaataaac tgcgtggcga
    10561 ccgctatatc tggctggtag tgctggggct atctctgttc agcattctgg cagtgtacag
    10621 ctccaccatt gcactttctt ttcaggaagc taatggcaat acggagtatt ttgtgctcaa
    10681 acacatcggt atcatgttgt ttggtgtggt catgatgtac ctggcacacc tgataccgta
    10741 ccgtttttac agcaggatct ctcagatatt gctatggctg tctattccgc tgcttattta
    10801 caccctgttc ttcggggttg agatcaatga tgcctccaga tggatcacca taccggtagt
    10861 aaaccttacc ttccagacca gcgacctggc aagatttgca ctgatcatgt acacagccag
    10921 actgctatcc agaaaacagg agctggtaca tagttttaaa gaagcatttg ttccggtcat
    10981 gctgccggtg ggcctggtat gtgcacttat ttttcctgac aacctcagta ctgcgtctat
    11041 actgttcgtc acctgtttgt tgctgatgtt catcggaaga gtgcatttta aatacattgc
    11101 tgtaactgct ttgtccggat tgttgctggt agcttccatc atcgctctgt catacctcat
    11161 gcctgacgta ggtcgttttg ctacatggca aagcagactt gaaagtttta ccggcggagg
    11221 agatgtgcct gatcaggtag tggtggcaaa gacatcaata gccatgggcg gagtactcgg
    11281 gcagggaccg ggtaacagta cccaggtaca ccatttcccg cttgcctatg ctgactatat
    11341 ctatgcactc atcattgaag aatacggttt gcttggtggg gtcgttatac tgttactctt
    11401 tcttattctg atgtacaggt gcatccgtat cgtggtatat gcaccctctt ctttcggagc
    11461 gctgctggct ataggcttag gactgagtct taccatccag gcattgatca acatgggtgt
    11521 ggcaacaagt ttgcttcccg ttacgggtct caccttgccg tttatcagca tgggaggaac
    11581 cagtttgtgg ttcaccagtt tgtcgttcgg tattattctg agcgtaagcc gtgagataga
    11641 actattgaaa ttacaggaac agggcgagct gcctgaagat gaaacaattc atgaaggaat
    11701 cgaataaaac atataaactg ctggtgagcg gtggtggtac cggcggacat atctttccgg
    11761 ctgttgctat tgccaatgca ttcaggagaa ggcatccgga aacagaggtg ctctttgttg
    11821 gtgcaaaagg aaaaatggaa atgcagaaag taccggcagc cggatatccc attgaaggac
    11881 tctggatctc cggattcagc agaagccttt cctggaccaa tctgctgttt ccggtcaaac
    11941 tcatacacag ctatttccgg gcaggagcca ttgtgcgcag gttccgcccg gatctggctg
    12001 tgggcaccgg cgggtttgca agcggccctg cactgaatgc agccatgcgt gcaggtattc
    12061 cggccatcgt acaggagcag aactcttttc ccggtatcac caacaagata ctgtctcgca
    12121 aagcacggaa agtatgtgtg gcatatgaag acatggaacg gttcttcccg gcaggaaaaa
    12181 cagaactgac aggcaacccg gtacgcaggg agatactgga taaccaggca acaagagagg
    12241 aaggaagggc acatttcggc atttcaacca gaaagaaagt agtgctggta gtaggaggaa
    12301 gtctgggaag ccgcacattc aacgaatgta tgatgacaga tctcaaccga atcagacagg
    12361 aagagataca tgtcatctgg caaaccggca ccctgatgtt cgatcaatgc agaaaagctg
    12421 catccggtat ggacaatgtt tcagtggtcg atttcatacc ggaaatggcc aaggcttatg
    12481 cggctgccga tgtgatcgtg agtcgtgccg gagccatcgc catctctgaa ttatgtgtgg
    12541 ttggaaagcc ggtcattctg gttccttttc cgtttgccgc cgaagaccac cagactaaaa
    12601 atgctcaggc attggtagat aaaggagcag cagttatgat cagtgatgcg gatgcacctg
    12661 ccaggctggt agatgcgatg ctgcacctct tgagagaccc ggaaaagcag gaacatttat
    12721 cggctgccat cagtcagctg gccgttacca atgcagctga aagaatagca gacatcatgg
    12781 aaaatattct gggaatagca taaatggaac agctcagcag gatatacttc ataggcattg
    12841 gcggcatagg catgagcgcc ctggccaggt attttcacgg gaaaggactg tttgtatcag
    12901 gatatgaccg gacagaaaca gaactgacca ggaacctgca acaagagggt atgaccatct
    12961 gctttgaaga tgatgttgcg ttgctggata aggatgccga tctggtggta tatacaccgg
    13021 ccattcctgc agatcatcaa caactgaact actacaggga tcatggctac caactgaaaa
    13081 aacgttcgca ggtattggga gaactgacgc acgatcattt caccattgct gttgccggtt
    13141 cacacggaaa gaccaccacc tcctccatga tcgcctggat acttcgcgac agcggatatg
    13201 actgcactgc atttctgggt ggcatctgcg tgaacttcga atccaacttc gtgcagggag
    13261 ccaatcaggt gattgtagtg gaagccgatg aatttgaccg ttccttccac acgctgcatc
    13321 ctgatattgc agtggtcaca gccattgaca gcgatcatct ggagatctac ggaacgcagg
    13381 aagcactgga agagaatttt cttgtttttg caaaacagat ccatgacaac gggttgctca
    13441 tacacaagca acaggtcaca gcattgcagg cgcataccgg ccacagatca acttattcat
    13501 tgcgggatga tgcggcagat ctgtatgctg tatccacaga gatcggagaa cagggcagcc
    13561 gggttattct cagcaatggc tttgatttcg tcctggagta tccgggt
//
LOCUS       DAOMAX010000052        13403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_554_length_13403_cov_4.218610, whole genome shotgun sequence.
ACCESSION   DAOMAX010000052 DAOMAX010000000
VERSION     DAOMAX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_13403_cov_4.218610"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..686
                     /locus_tag="PK742_04475"
     CDS             48..686
                     /locus_tag="PK742_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75878.1"
                     /translation="MKNLLLVFLFIGSANITYCQNQIANDRIFRRTFSKTELEDLQLL
                     FDFFNQTICDSNEVLEDCYQAYFIRLNEAAEDGVMYLHIPFEEQQEVYKKLSDSTFRE
                     IWVFGEAWFQETPDHILRTIYFNANGDFMRFLKKASRKDAFINVCYESAKLTGMPGAT
                     VVAEIYRNNNTFDIEDVKVKFVIAVMNLTLNDQYKRKEMIRPDDRSKIKSKE"
     gene            819..1943
                     /locus_tag="PK742_04480"
     CDS             819..1943
                     /locus_tag="PK742_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQU75879.1"
                     /translation="MKNFKAGTYISQGSFKSFVPNEINRIWQIDDPEVIGLLSKADRQ
                     LGRLDMYSEYIPNLDLFIRMHVIKEATQSSKIEGTRTNIEDALQNKHNVAEERREDWE
                     EVQNYIKALNQAIQSLEDLPFSSRLIRQAHKTLLHGVRGKNKQPGAFRNSQNWIGGAT
                     LSDATFIPPPASEISRLMGDLEKFAHNEEVFFPDLLKIALIHYQFETIHPFLDGNGRV
                     GRLCITLYLVEKGLLKKPVLYLSDFFEKNRQLYYDNLMKTRLDNNIKQWYKFFLVGII
                     ETAKSSIATFDNILKLQKEINEKINTLGSRAGNANLVIDSLYQNPLTNAQQVASLTGL
                     SLPTAYKIVSDLETLGIIREFTGAQRGKQYWFKDYLDLFK"
     gene            2046..2687
                     /locus_tag="PK742_04485"
     CDS             2046..2687
                     /locus_tag="PK742_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQU75880.1"
                     /translation="MSAKPLKYYSRPEENLNIATHGLGVLLSIVALVLLIGKARAAAT
                     PYYLVSFTVFGISLILLYTASTLYHASVRPELRSKFKVLDHAVIFVLIAGTYTPFTLI
                     TLHGWVGWTIFGVVWGIAAVGMVFKLFHTGRYEKISTIAYVAMGWIIVFAIVPLIQNL
                     ASGGLWWLLAGGACYTLGAVLYRMHNLPYNHAIFHVFVLLGSFSHFMAVYKFV"
     gene            2811..4184
                     /locus_tag="PK742_04490"
     CDS             2811..4184
                     /locus_tag="PK742_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75881.1"
                     /translation="MTRIRLLILLTTFSFCGRSIAQCSDVSLLVSSSDTTYVQLYHPA
                     LFLIPSGDANICYWTVTTFSGELVHSDTTSGDFEAQSFSYFDHSVPVTDSMKVTLIIE
                     NPVEDITCAINDTLFWEETEVIPGVFFGNWAILSDNGGVEGAAYSVTFQVDMSEVSDP
                     FTTPEVNGTFNDWCGGCAPMTDDDADNIWQITIDLEAGFYEYKYAADTWTIQESLTPG
                     DPCTMTTDIFTNRTLDVSEDMVLEAVCWGSCEACETETTVYSVTFMVNMNEVSEDFTT
                     PEVNGTFNDWCGGCAPMTDDDADNIWQITIDLEEGSYEYKFAADTWTIQESLTPGDPC
                     TVTADIFTNRQLDVFEDIVLNAVCWGSCEACDTTGGPTSSSLFELNDQVQLSPSVVDD
                     GFVLSGLTQTYSFSITDLNGRLMDRYRNVPAGQKIMTDTYAPGLYILILTSETGEVIA
                     MKKFFKE"
     gene            4321..5667
                     /locus_tag="PK742_04495"
     CDS             4321..5667
                     /locus_tag="PK742_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75882.1"
                     /translation="MSVLRRKPLRRLKEQVLKNTIGTLGVLICTMLLVHDLTACSMYK
                     ITTDGKTMVGCNYDAWFTTPKIWFENARTPEEYGAGFTGAREVSNNRTAPQSGMNEAG
                     LAFSRLVAYYPAQENPFRDRLVIADEAAYLSDILHTCANVEEAREYIERFDHSIFLED
                     VFIYVDGSGKYLIVEPYQIISGDDASYLLSNFCPSITDNEQARKLERFRNGEDFLSEH
                     TLSSSLSFCRSLSDTMHVCRNKKGDGTLLTSIWDTKERLLVLYFYHSYDQEVRFDLTT
                     ELSKGDHVMAIPELFPENAEFEELKSYKTPFNTPELRITLALLGAFLSITTLYFVRQI
                     WRNRHSRSAGNSLNLVAGLNILLTGYLFILATNVSVFYFDAPYKHYSSGLISASSYIP
                     FLVLLAIVPVTRFTIRNLRAEKRYLWFKMLLVTNNLIYFILLVGFGYWGLFTFWKQ"
     gene            5859..8702
                     /locus_tag="PK742_04500"
     CDS             5859..8702
                     /locus_tag="PK742_04500"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU75883.1"
                     /translation="MILTACSLSVRAQDPIAIPFTSTEIYLPVTTYFVKQASDGLIWF
                     STDAGVFSYNGYNITNYSSANGLSDNEIFRIYEDNKHRLWFLGLNGKCSYYYNGEIFN
                     EENDPMLQKLRFNTMISAETEDRDGNLYIASREGQIYRITPDNQVTMTENENGVFFLY
                     AFRDTVLNIPQYFNKKIGRLPRGGQMGDLVIASIEREIYVLNRDNSFTLITTLPEESQ
                     EIIFLGVKADSTIYAGTRNGLYQVSVPDGKILSVNFRGLSITSVTTDDDGQLWLSTLD
                     KGLFLVPSLYNDVYNLSSGWPGNRIFSIYKDKQDSLFISMPGNWYSVIAPDSSFQHFQ
                     IPGKRSLKLEISQIRKFGDDTWIVAKSGIYRLTPTGNKLYRQYANDLYVYDDTILIAQ
                     QNILKFSLPEYEAFDREVAPQPSRTDPYLLIPSRCGQIQPGRTGEFWFATVHGLYCYR
                     NGQVIDFGDHDIRLSNYVRGLEYDSLRNILYVATNTTGILMLENDQIVAEIDTRCGLP
                     FNESYAVKLDDKGVLWASSSNELIRVLWQDGKPLVDMVGRKLGLYSSRILNMEFRGNE
                     LYLGTETGLIRIDTSRVVPTTAPRLYLRQIRAGQTVIADIDHIQLPYRQNGITIEYTG
                     IYFRRNAPLQYQYLLEGYDKEWQTTTDRSLNFRSLPPGDYIFHVKAVNTNGDTSETLS
                     IPFSIATPFWQTTWFIALTGLAFLGLITLLWRWRLQHVHRRYEKEKSIIETGKKLAEL
                     EHKTFKLQMNPHFIFNTLNSIKGYYAEQNTVQANEYITKFSRLLRNILESDAQYISLE
                     QEIQALSLYMDLMQLRYDNSFSYHIDTTNVETASTAIPPMLLQPFVENSIIHGIHPLT
                     IPGELNISFRKSGKRLLCTITDNGVGRTASHLQNRNKEHQSKGMQIIRDFFAAMNSQL
                     EEDAFTMQIRDLEKDGKPGGTVVEITMLLITLL"
     gene            8707..9453
                     /locus_tag="PK742_04505"
     CDS             8707..9453
                     /locus_tag="PK742_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQU75884.1"
                     /translation="MNTNSIRSFIVDDETQSRSALRQELQLHCTEVENVGEAGNIPDA
                     VEAINKLQPDLLFLDIKLRDGLGFEILDKLEHRNFHIIFTTAYSEYALKALKLNALDY
                     LLKPIDSQELIQAIAKVMEMPVRNKPMLKQPLPDQSGRISLNTSEGTFVVALSDITHC
                     TAYGNYCFIHLIGNRRIMITRTMKDLEKQLGEFGFERIHHSHIVNLSHVVSYKNKDGG
                     TVVLTDDTQLPISTRKRQNLLTYFDTISLK"
     gene            complement(9512..10906)
                     /locus_tag="PK742_04510"
     CDS             complement(9512..10906)
                     /locus_tag="PK742_04510"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75885.1"
                     /translation="MKNKCISLLFAFLVLPATLLACDLSSFTVNSVADLGGGYYEFNV
                     TFCAGSGQNAYRYGADQNTYTWAIRVDNGAVVTSAPAFLTSPNTGSIYFSDTISYMGY
                     HYLVYDYSSGGNGWWTCIDGGCGPVQSTCIDVTFTTYGMPNTILGGGAESAGIVIAGL
                     GYGCNDEPDMIQHLLVPEAHAGNDTQVCPGGTVTLDAGASDGTPPYTYLWSTGATSSS
                     ISVAPAVSTTYSVTVTDGNGNTATDDVTVSIASTPTANAGADKQIYIGYGATCVSLNG
                     SATGGTSPYTYAWSNGSTSAIPSVCPATSTTYTLTVTDANGCASTDAVEVAVENIVCG
                     PGKVYVCHYGTTKCYKTNQVNSHLNHGDYLGTCDGMRLADVDEQPMLSAYPNPACNEV
                     SLLFHLEEETAVTIEFYSMSGQLVARPLMNNLRESGDWLEVIALHDMEPGMYMVRLVS
                     DAGDQYMTSLMISR"
     gene            complement(11030..12910)
                     /locus_tag="PK742_04515"
     CDS             complement(11030..12910)
                     /locus_tag="PK742_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQU75886.1"
                     /translation="MLLRIMVVLIMAFLLACNRQPVPAYMTSLDSLEDFLQLADNDAP
                     NASIRSVKVLLDLHTDSMYFIDSDIFPSHYEFANRYLEHTENKYDFFVGQYGEDSVRH
                     LHMVNLSWFVEKNLFLFEFGPYDLADCAAVSKVYDRLMETTFIGYRLAFYPNVSKWED
                     CERIPWISPQTISFLYPYMAYNTGEAYGYLRIFDDGEKDPQADPTDIIVLTRLPFDIP
                     LVAGIISSAPQNSLSHINVLSRNRGTPNVYIRYASENSDLQQMEGKLVHLVARANSYI
                     LEPASESDARAFWKKWQPEDQWQLSSDHSVQRLLNPSETDLCSVSAVGGKAANFSILH
                     DMGTVPLPEGAFIIPVYYYYEHLEKNGLLPELKAILDDPLLKTDPSYRKRRLKDLRTA
                     IMEAPVDLKLLHAIDSALHHFSDYPSYRFRSSTNAEDLDFFSGAGLYKSRSAHAEEGK
                     AGIADAITAVWASLWSDRAYVERDFFGIDQHTVEMAILVHRSFPREEANGVLITRNPY
                     NVNPGYQVEVHFGGTGVVRSFDDGPDEVIIYPIDLVRNRYTIEYRSKSDFGRMRSSVM
                     SDNELAALADQARNIQERFVEVYQPDDPSAFAVDIEFKIDEVDGIRRLYIKQARPFPA
                     GR"
     gene            13003..>13403
                     /locus_tag="PK742_04520"
     CDS             13003..>13403
                     /locus_tag="PK742_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQU75887.1"
                     /translation="MITVQEICRVLEAWAPPVLQEEYDNSRLLTGNRKQEVTGILVTL
                     DVTEAVIEEAIARKCNLVISHHPVIFGGLKKLTGDNYVERTVLLAIKNDIALFAIHTN
                     LDNVRGGVNQAIAAKLQLQETRVLRPIKGLL"
BASE COUNT         3763 a         3145 c         3026 g         3469 t
ORIGIN      
        1 gactacattc atacagttac tgaggtcacc taattcgaat actactcatg aaaaacctgc
       61 tactagtatt tctttttatt ggttcggcga atatcacata ctgtcagaat caaattgcca
      121 acgacagaat attcaggcgc actttttcta agactgaatt agaagacctt cagctattgt
      181 ttgatttctt taaccaaacc atatgcgatt cgaatgaagt attggaagat tgctatcaag
      241 cctatttcat aagactaaat gaggcagctg aggatggtgt aatgtatttg catatacctt
      301 tcgaagaaca acaggaggtc tataagaaac tcagtgattc cacatttcgg gaaatatggg
      361 ttttcggaga agcctggttt caagaaacac ccgatcacat tttacgtaca atctatttta
      421 atgcaaatgg tgatttcatg aggttcctga aaaaggcgag cagaaaagat gcgttcatta
      481 atgtctgtta tgagtcagcc aaacttactg gcatgccggg tgctactgtt gtggcagaaa
      541 tatacaggaa taacaatacg tttgacatag aagatgttaa agtaaagttt gtcattgcgg
      601 tcatgaatct gaccctgaac gatcagtaca agagaaagga gatgatccgg cctgatgatc
      661 gatctaaaat aaaaagtaag gaataggcat tcgatcaggt ttggaatgct cattaaccac
      721 tattttattt aatctttttg tccttttatt tatttatgca gacttttatt taactttttt
      781 attcttttct taaattaggc tatctttgtt gaagaaacat gaaaaacttc aaagcaggaa
      841 cctatatctc tcagggcagc tttaaaagct ttgtgccaaa tgaaataaac cggatctggc
      901 aaattgatga tccggaagta attggtctgc ttagcaaagc tgacaggcag ttaggtcggt
      961 tggatatgta ttccgaatac attccaaacc tggatctttt tatcagaatg catgtaataa
     1021 aagaggccac acaatccagc aaaattgaag gcaccaggac aaacattgag gatgcattac
     1081 agaacaaaca caatgttgca gaagaaagaa gagaagattg ggaagaagtt cagaattata
     1141 taaaagcatt gaaccaggcc attcaatcac tggaagatct gccattttct tccagattga
     1201 tcaggcaagc acataagact ttattgcatg gtgtaagagg gaaaaataaa caacccggag
     1261 ctttcagaaa cagtcagaac tggatcggag gagcaaccct atcagatgcc acatttatac
     1321 cacctccggc gtcagaaatt tcaaggttaa tgggagacct ggagaaattc gcacataacg
     1381 aagaggtctt ctttcctgat ctgctaaaaa ttgctttgat ccattatcag tttgaaacga
     1441 tacacccatt tctggacggg aatggccgag taggccgatt atgcatcaca ctttatcttg
     1501 ttgaaaaagg cctgcttaaa aaacctgtat tgtatttgtc agatttcttt gaaaaaaaca
     1561 ggcagctata ttacgacaat ctaatgaaga ccagattgga taataatatc aaacagtggt
     1621 acaaattttt tctagttggt attattgaaa cagctaaaag cagcattgca acttttgaca
     1681 atattctaaa acttcagaaa gagatcaatg aaaagatcaa tacacttgga agccgggccg
     1741 gcaatgcaaa cctggtcatt gacagcctat atcagaatcc ccttacaaat gctcagcaag
     1801 tagccagtct gactggattg tcattaccaa ctgcttacaa gatcgtatca gatctggaaa
     1861 ctcttggtat aattcgagaa tttacaggag cacaacgcgg taaacaatat tggtttaaag
     1921 attacctgga cctgtttaaa taaatatgtc ttttttagct gcagttggca tccccgccaa
     1981 ccgtccatcc caccccattc accatcccca aactccgtaa tttcgtcctg gaatttccca
     2041 tttgcatgtc ggccaaacca ctgaaatact actcccgtcc ggaagagaat ctcaacatcg
     2101 ctacccatgg tctgggtgtg ctgcttagca tcgtggcgct tgtgctgctg atcgggaaag
     2161 cccgtgcagc tgcaacacct tattacctgg tgagctttac ggtatttggc atcagtttga
     2221 tcctgctcta taccgcctct accctgtatc atgcatcggt cagaccggag ctgcgttcaa
     2281 agttcaaagt actggaccac gcggtcatct tcgtgctgat agccggcacc tacaccccgt
     2341 tcaccctgat cacattgcat ggctgggtgg gctggaccat cttcggggtg gtctggggca
     2401 tcgctgcagt gggtatggtc ttcaagctgt tccacaccgg caggtatgag aagatatcta
     2461 ccatcgccta tgtggccatg ggctggatca tcgtgtttgc cattgtacca ctgatacaga
     2521 acctggccag cggcgggctc tggtggctgc tggcaggcgg tgcctgttat accctcgggg
     2581 cagtgctgta ccgcatgcat aatctgccat acaaccacgc catctttcat gtgtttgtgc
     2641 tgctgggtag tttttcgcat ttcatggctg tttataagtt tgtctgaaca tcagcctgtt
     2701 cgggtctgtt tattgtcagg ataatcagga aggatggatt gcttcaggta ttactttact
     2761 tttgcgcatc agaaaaaaat aaccacttac gccggcataa tttgaaacca atgaccagaa
     2821 tcagactact tattctactc accacatttt ccttttgtgg cagatcaatt gcacaatgtt
     2881 cagatgtgtc tctgcttgtc agttcttcag acacgaccta tgtacaactc taccatccgg
     2941 ccttgttcct gatcccttca ggcgatgcca atatctgtta ctggactgtt accacttttt
     3001 caggtgaact cgtgcacagc gacaccacaa gcggagattt tgaagcgcaa agcttttcct
     3061 actttgacca ttctgttccg gtcaccgatt ccatgaaagt caccctgatc attgaaaacc
     3121 ctgtggagga tatcacctgt gcgatcaatg acaccttgtt ctgggaagag acggaggtca
     3181 ttccgggtgt attctttggc aactgggcta tacttagtga taatggcggt gtagaaggtg
     3241 ctgcttattc tgtcaccttc caggtagata tgagtgaagt atctgaccca ttcaccaccc
     3301 ccgaagtgaa cggcaccttc aacgactggt gtggtggatg cgccccgatg accgacgatg
     3361 atgccgataa catctggcag atcaccatag acctggaagc gggtttctat gagtataaat
     3421 atgctgctga cacctggacc atacaggaat ctcttacacc cggagatcct tgtaccatga
     3481 ccactgatat ctttaccaat cgtacactgg acgtttcaga agacatggta cttgaagccg
     3541 tttgctgggg aagctgtgaa gcctgtgaaa cagaaacgac cgtgtattct gtcaccttca
     3601 tggtaaacat gaacgaagta tcagaagact tcaccacccc cgaagtgaac ggcaccttca
     3661 acgactggtg tggtggatgc gccccaatga ccgacgatga tgccgataac atctggcaga
     3721 tcaccataga cctggaagaa ggttcatatg agtacaaatt tgccgctgac acctggacca
     3781 tacaggaatc tcttacacca ggcgatccct gtaccgttac agcagacatt ttcaccaacc
     3841 gtcaactgga tgtttttgaa gatatcgtac tgaatgcagt ttgctgggga agttgtgaag
     3901 cgtgtgacac tacaggcggc ccaacttcat catcgttgtt cgagctgaac gatcaggtac
     3961 agctgtcacc ctctgtagtt gatgatggat tcgtactcag cggattgacg cagacatatt
     4021 cattcagtat aacagatttg aacggtcgat tgatggatcg ctatagaaat gttcctgccg
     4081 gtcagaagat catgacagat acttatgctc cgggtctata tatacttata ctgacatccg
     4141 aaaccggtga ggtcattgct atgaagaaat tcttcaaaga atagcccatc ctcaccctat
     4201 tctgtttgac cagatcgttt ggttaccggt tgtacttgta aaaacatcag taccggaatc
     4261 agaaatacgc atttccggcg ttcttttctc ttttgatcat ggtgattaaa agaactgaaa
     4321 atgtctgtcc tgcgcaggaa acctctgaga aggctcaaag aacaagtact aaaaaatact
     4381 ataggtacac tgggggtatt gatctgtacc atgctgctgg tgcatgatct aacagcctgc
     4441 agcatgtaca agatcacaac cgatggcaag accatggtcg gctgcaacta cgatgcctgg
     4501 ttcacgacac ctaagatctg gtttgaaaat gcacgtacac cagaagaata tggtgccgga
     4561 ttcaccggag ccagagaggt ctccaataac agaactgctc ctcaatcagg aatgaacgaa
     4621 gccggtctgg ccttttcccg attggttgcc tactaccctg ctcaggaaaa cccattcaga
     4681 gacaggctcg ttattgccga tgaagcagcc tatctgagtg acatcctgca tacctgtgca
     4741 aatgtggagg aagcccgtga atatattgaa cggtttgatc acagtatttt ccttgaggat
     4801 gtcttcatct atgtggatgg gagtggaaag tatctgatcg ttgaacccta tcagatcata
     4861 tccggtgatg atgcaagcta cctgctttct aatttttgcc catccataac agataatgaa
     4921 caagccagga aactggagag attcagaaac ggggaagatt tcctgtcaga acataccctg
     4981 agttcatcgc tgtcattctg caggtctctg tcagatacca tgcatgtttg caggaacaaa
     5041 aaaggcgatg gcacgttgct gacatccata tgggacacga aggagcgcct gctggtactc
     5101 tatttctatc attcctatga tcaggaggtc cggtttgacc tgaccacaga actgtcaaaa
     5161 ggtgatcatg taatggccat tccggagtta ttccccgaga atgccgaatt tgaagaactg
     5221 aagagctaca agacgccatt caatactcca gaactcagaa taacacttgc actgctggga
     5281 gcttttttaa gcattaccac actttacttt gtgcgacaga tatggagaaa caggcattcc
     5341 aggtctgccg gcaattcgct gaaccttgtt gccggtctga atattctgct taccggctat
     5401 ctgttcatac tggcaaccaa tgtatccgtt ttttactttg atgctccgta caagcattat
     5461 tcttcaggtt tgatcagtgc ttcatcctac ataccattcc ttgtactgct tgctattgta
     5521 cctgttaccc ggttcacgat ccggaacctg agagctgaaa aaagatacct gtggttcaaa
     5581 atgctgttag tcacaaataa cctgatctat ttcatactct tggttggctt tggctactgg
     5641 ggacttttta ccttctggaa acaatagagc ctttccctct tcaacggggt atcagccaac
     5701 tttttttaag actttaaaca gataagtgcc gggcatttcc tgataagtgc agcgtcttct
     5761 tcagaatgtg ttaaacacaa gtgtgtacca gacataatac atactaaaat cgcccctaat
     5821 tctgcttatt ttgcacttct gtgcggattt ttatttgcat gatattaaca gcttgttcgc
     5881 tctctgtgcg tgcacaggac cccattgcta ttccattcac tagtaccgag atctatctgc
     5941 cggttactac ctactttgtg aaacaggcca gcgacggact catctggttc agcaccgatg
     6001 caggtgtatt cagttacaat ggttataata tcaccaatta ctccagtgcc aacgggcttt
     6061 ctgataacga gatctttcgc atttacgaag acaacaaaca ccggctctgg tttctgggac
     6121 tgaacggaaa atgttcttac tactataatg gagagatctt caatgaagaa aatgacccta
     6181 tgctgcaaaa gcttaggttc aacacgatga tatctgctga gaccgaagac cgtgacggca
     6241 acctgtacat agcttcacga gaaggacaga tatataggat cacacctgac aatcaggtga
     6301 ccatgacaga gaacgaaaac ggtgtattct ttttgtatgc gttcagagat accgtgctga
     6361 atataccgca atacttcaac aagaagatcg gccgtctgcc cagaggtggc caaatgggtg
     6421 atttggtcat agcatccatt gaacgagaaa tatatgtatt gaaccgtgat aactccttca
     6481 cattgatcac aaccttaccg gaagaaagtc aggagatcat ttttctggga gtgaaggctg
     6541 acagtactat ttatgcaggt acccgcaacg gtttatatca ggtgtcagta ccagacggaa
     6601 agatcctgtc tgtaaatttt cgtggactga gtatcacatc tgttacgacc gatgatgacg
     6661 ggcagctctg gctttctacc ctggataaag gactctttct tgtaccctca ctgtataatg
     6721 atgtatataa tttaagcagt ggctggcccg gcaacagaat attcagcatt tataaagaca
     6781 aacaagactc actgttcatt tccatgccag gaaactggta ctctgtcatt gctcctgata
     6841 gtagttttca gcactttcag atccccggca aacgaagcct gaagctggag atctcgcaga
     6901 tcagaaaatt tggagacgac acctggatcg ttgccaaatc aggtatctat cggcttaccc
     6961 ctaccggcaa taaattgtac cgccagtatg caaatgatct gtacgtttat gacgatacga
     7021 tactgatagc ccagcagaat attttgaaat tcagcctgcc tgaatacgag gcatttgaca
     7081 gagaagttgc accgcagcca tccaggacag atccgtattt gttgatcccc tcccgttgtg
     7141 gacagataca accgggccgt actggtgaat tctggttcgc aaccgtgcat ggcttgtact
     7201 gttaccgcaa tggccaggtc atagactttg gcgatcatga tatcaggtta agcaattacg
     7261 taagaggtct ggaatatgac agcctccgga acatcttata tgtagctacc aatactaccg
     7321 gtatcctgat gcttgagaac gaccagatcg ttgcagagat cgatacccgt tgcgggcttc
     7381 cattcaatga aagctacgca gttaaacttg atgacaaagg agttctctgg gcttccagca
     7441 gcaatgaact gatcagggtt ctctggcagg atggaaaacc tttggttgac atggtgggca
     7501 gaaaactggg gctctactca tccaggattc tgaacatgga attcagagga aatgaactct
     7561 atctcggaac tgaaaccggg ttgatccgca tagacacctc acgggtagtt ccaaccactg
     7621 ctcccagatt atacctgcgg cagatccgtg cagggcagac cgtgatcgct gatattgatc
     7681 atatccaact tccgtataga caaaacggta tcaccataga atacaccggc atttatttca
     7741 gacggaatgc tccccttcaa tatcaatacc tgttggaagg atacgacaaa gaatggcaga
     7801 ccacaaccga ccgatcattg aatttcagat ctcttccacc cggagattat atattccatg
     7861 tcaaagccgt caataccaac ggagatacca gtgagacctt atccatcccg ttttccatag
     7921 caaccccatt ctggcagacc acctggttca ttgccctgac aggtctggct tttctgggtc
     7981 tgatcaccct gttgtggcgc tggcggctgc aacatgtaca ccgcaggtat gaaaaggaaa
     8041 aatccatcat agaaaccgga aagaagctgg cagaactgga gcacaagacc ttcaagttgc
     8101 agatgaaccc tcacttcatc ttcaatacgc tgaacagcat caaaggctat tatgcagaac
     8161 aaaatacagt tcaggccaac gaatacatca caaagttctc ccgtctgctc cgcaatatcc
     8221 tggaaagtga cgcacagtat atctctctgg aacaggagat acaggctctg agcctgtaca
     8281 tggacctgat gcagctgcgc tacgataatt ccttctccta tcacattgat accacaaatg
     8341 tagagacagc ttccaccgcg atcccgccaa tgttgctgca accctttgtg gaaaacagca
     8401 tcatacatgg cattcatccg ctgaccattc cgggtgaact gaatatttct ttcagaaaat
     8461 caggcaaacg actgctgtgc accatcactg ataatggtgt tggcagaaca gcatcacacc
     8521 tgcaaaacag aaacaaagag caccagtcga aaggaatgca gatcatccgc gatttcttcg
     8581 ccgccatgaa cagccagctg gaagaagatg cttttacaat gcagatccgc gatctggaga
     8641 aggacggaaa gcccggcgga actgtggtag agatcactat gctcctgatt accttattgt
     8701 aacaatatga acacaaatag tataagaagc ttcattgtag atgatgaaac ccagtcaagg
     8761 tcagcactgc gtcaggaact gcaactccac tgtacagaag tagagaacgt tggcgaagcc
     8821 gggaacatcc ccgatgccgt ggaggccatc aataagctgc aacccgatct gttgttcctg
     8881 gatatcaaac tacgagacgg cctgggattt gagatcctgg ataaactgga acaccgcaac
     8941 ttccatatca tctttacaac tgcctattca gaatatgcac tgaaagcact caaactgaat
     9001 gctctggatt atctgctcaa acctatagac agccaggaac ttattcaggc aatagccaag
     9061 gtcatggaga tgcctgtccg caacaagcca atgttgaaac aaccgctgcc tgaccagtcg
     9121 ggtcgaatca gtctgaatac ctccgaaggc acttttgtag ttgctctgtc tgacatcaca
     9181 cactgcacgg catatggcaa ttactgtttc atacacctga tcggtaatcg tcgcatcatg
     9241 atcacccgca ccatgaaaga cctggaaaaa caactgggcg aattcggatt tgagcgcatc
     9301 caccattctc atattgtcaa cctcagtcat gtggtcagct ataagaataa ggatggtggt
     9361 acagtggtgc tcactgatga tacccagttg cccatctcca cccgtaaacg ccagaacctg
     9421 ctcacctatt tcgacaccat cagccttaaa taaaaaatgc ccccggctat ggctccgggg
     9481 gcatcagggt aaagggggat ctgattatct tttatctgct gatcatcagc gaggtcatat
     9541 actgatctcc cgcatcactg accagtctga ccatatacat tccgggttcc atgtcatgca
     9601 gtgcaatgac ctcgagccag tcgcccgact cacgcaaatt gttcattaac ggacgggcta
     9661 ccaactgacc tgacatgctg taaaattcaa tagtgacagc tgtttcttct tcgagatgaa
     9721 agagcagact tacttcgttg caggcagggt tcgggtaggc agacaacatg ggttgctcgt
     9781 ccacgtctgc aagtcgcata ccatcacagg taccgaggta atcgccgtga ttaagatggc
     9841 tgttcacctg attggtctta tagcattttg tagtaccata gtggcacaca tacaccttac
     9901 cggggccaca tactatgttt tcaactgcca cctcaacagc atctgtggaa gcacagccat
     9961 tggcatctgt tacggtcagg gtataggtag tggaggtggc aggacatacg ctggggatag
    10021 cagaggtgga gccgttactc caggcatagg tataaggaga tgttcctccg gttgctgatc
    10081 cgttgagcga aacacaggta gcgccatacc cgatatagat ctgtttatca gcaccggcat
    10141 tagctgtcgg tgtggaagca atagatacag taacatcatc tgttgctgta tttccattac
    10201 catctgtaac cgttacagaa taggttgtgg atacagccgg agctacactg atagaagaag
    10261 aggtagcacc cgtgctccag agataggtat agggaggcgt gccatcagat gcacccgcat
    10321 ccagagtgac ggtaccaccc gggcatacct gggtgtcatt tccggcatga gcttccggta
    10381 cgagcagatg ctggatcatg tccggctcat cgttgcatcc gtacccaagt ccggcaatta
    10441 cgatacctgc tgattctgca ccgccaccca gaatggtgtt gggcatacca taggtagtga
    10501 aggtcacatc gatacaggtt gactgtaccg gaccgcagcc accgtctatg caagtccacc
    10561 agccgttgcc gccactggaa taatcatata cgagatagtg gtatcccata taggaaatgg
    10621 tgtcgctgaa atagatggat ccggtgttcg gactggtcag gaatgcaggg gcgcttgtta
    10681 ccactgcacc attgtccacc cggatagccc aggtataggt gttctggtca gcaccataac
    10741 gataagcgtt ctgaccggaa cctgcacaaa aggtcacatt gaattcgtag tagcctccac
    10801 ccagatcagc tactgaattg acagtaaaag atgacaggtc gcaagccagc aatgtggcag
    10861 gcaaaaccag gaaggcaaac aggagtgaaa tacatttgtt tttcatggtt gtaggtttta
    10921 tgaaaacaaa tgtatctggc ctgccaactc aaacggtaag attcagacaa gtggccggta
    10981 aggatgtatg caattcatct ttctgaggat aagtgttttg gaggggaggc tatcgaccgg
    11041 caggaaaagg acgagcctgt ttgatataca gtctcctgat gccatccacc tcatcgatct
    11101 tgaattcaat atcgaccgcg aatgccgatg gatcgtccgg ctgatacact tccacgaatc
    11161 tttcctgaat gttccgggcc tgatctgcaa gagcggcaag ctcattatcg gacattaccg
    11221 aagatctcat tcttccaaaa tctgatttac ttctgtattc aatagtgtac cggttccgga
    11281 ccagatcgat cggatagatg atgacctcgt ccggcccgtc gtcaaatgat cttactactc
    11341 ctgttccacc aaaatgcact tccacctggt atccgggatt tacattatac ggattccggg
    11401 taatcagaac cccgttcgct tcctcacgcg gaaaggagcg gtgcaccaga atggccatct
    11461 ccaccgtgtg ctgatcgatg ccaaaaaaat cacgttccac ataggcacgg tcgctccaca
    11521 ggcttgccca tactgcagtg attgcatcag caatgccggc ttttccttcc tctgcatgtg
    11581 ctgatctgga cttatacaat ccggctccgc tgaaaaaatc cagatcttct gcgttagtgg
    11641 atgacctgaa ccgatacgac gggtagtcag aaaaatgatg cagggctgaa tcgatcgcat
    11701 gcaacaattt cagatctacg ggagcttcca tgatggcagt ccgcagatct ttcagccgcc
    11761 gttttcggta tgaaggatcg gtcttcagga gaggatcatc caaaatagct ttcagttcgg
    11821 gcagcagacc attcttctcc agatgctcat aataatagta aaccggaatg ataaaagccc
    11881 cttcgggtaa tggcaccgta cccatatcat gcaggatgga aaaattggcc gccttccctc
    11941 caaccgctga cacagaacac agatcggtct ctgaaggatt aagcaatcgt tgaacagaat
    12001 gatctgaaga tagttgccat tgatcttctg gctgccattt cttccagaag gcccttgcat
    12061 cactttcact ggcaggttca aggatatagc tgttggcccg agctaccagg tggaccagct
    12121 taccttccat ttgctgcagg tcactgttct cagatgcata gcggatatac acattcgggg
    12181 ttcccctgtt ccggctcagc acattgatgt gacttaaaga attctgcggt gcgctggaga
    12241 tgatcccggc taccaatggg atatcaaagg gcaatcttgt gagaacgatg atatcagttg
    12301 ggtctgcttg cggatccttc tctccatcat cgaaaatccg gagatacccg tatgcttcgc
    12361 ctgtattata tgccatatag ggatacagaa aactgatggt ttgaggtgag atccagggta
    12421 tccgttcgca gtcctcccat ttggacacgt tgggatagaa cgccagtctg tagcctataa
    12481 aagtagtctc catcagccga tcatacacct ttgaaaccgc ggcacaatcg gccagatcat
    12541 aagggccgaa ttcgaacaga aagagattct tctccacaaa ccagctcagg ttgaccatgt
    12601 gcagatgccg cacagagtct tcaccgtatt gtcctacaaa aaaatcatac ttgttctccg
    12661 tatgctccag atagcgattg gcaaattcat agtgactggg aaaaatgtcg gaatcaatga
    12721 aatacatact gtcggtatgc aggtccagaa gcactttcac agagcgaatg ctggcattgg
    12781 gtgcatcatt atcggccagt tgcaaaaaat cctccaggct gtccagactg gtcatgtaag
    12841 ccggaacagg ctgtctgttg caggctaaca gaaaagccat gatcagaaca accattatcc
    12901 ggagcagcat gatataaatg taccaatgcc aaagaaggaa ttcagcaaat tccaataatt
    12961 tgaaatgaaa cttacaaaag ggtaatagat taatttcgta gcatgatcac tgttcaggaa
    13021 atctgcaggg tgctggaagc atgggcacca ccggtgctgc aggaagaata tgacaacagc
    13081 aggctgctga caggaaaccg gaagcaggaa gtgaccggca tcctggttac tttggatgtt
    13141 accgaagctg tcattgaaga ggcgatagcc agaaagtgta acctggtgat cagccatcac
    13201 ccggtcatat tcggcggtct caaaaaactg acgggcgata attatgtgga gcgcacggtg
    13261 ttgctggcca tcaaaaatga tatagccctg tttgccatcc ataccaatct ggacaatgtt
    13321 agaggtgggg tgaatcaagc cattgctgca aaactgcaat tgcaggaaac aagggtgctt
    13381 cgtcctataa aaggattgct caa
//
LOCUS       DAOMAX010000053        13189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_574_length_13189_cov_3.750343, whole genome shotgun sequence.
ACCESSION   DAOMAX010000053 DAOMAX010000000
VERSION     DAOMAX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_574_length_13189_cov_3.750343"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /gene="ccsA"
                     /locus_tag="PK742_04525"
     CDS             complement(<1..2217)
                     /gene="ccsA"
                     /locus_tag="PK742_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQU75888.1"
                     /translation="MEEIQSPVVQAINPWMGQAGHFLVLLALVGAAWSAVCYWLSVRE
                     KDLTSSLEWRRLARWGFLTHAASIAGMVLVLFFMIFGHHFEYQYVWQHSSRDLPMKYI
                     LSCFWEGQEGSFLLWSFWVMVLGLVLMRRSGEWEGRTMSILSLQQVFLLSFLTGVYVF
                     GKKIGSSPFLLLEDALTQDPVFLFSDYMQFIQDGTGLNALLQNYWMVIHPPVLFLGFA
                     SITVPFVYAMAGLWSGRYKDWIAPAIPYSLFSGMVLGTGILMGGAWAYEALSFGGFWA
                     WDPVENASLIPWLVIVAALHTMLVSKSTGHALRANFILVSLSFFFVLYASFLTRSGIL
                     GDTSVHSFVVSGLENHLLLYLFVFTFISCWLIIRHWKKIPAEKKEEATASREFWMFIG
                     SLVLLFSALHVIFFTSIPAFNHLFAWINRIIGTAIKTDFTAPVDPVHYYTNVQIWVAI
                     IIAVLTAFGQFLRYRQSDMKKFWRSLIISFGLTIPVTIFSARALEYPLFLRSGNIPLP
                     DPWVLLLFMSTFTVLANLQYFITVMKGRVRVSGGSVSHVGFGILLLGILISNASQQVI
                     SKNTMGINYGDSFDQEFKRDNILLYKDSPVVMGQYLVTYAGDSTDNRKTFYKVRYEKL
                     DDKTGEVSSSFILYPYLLTDKKSQQLNPNPDTKHYLTHDVFTHVSSIPARNQPAQDVI
                     EKDHTIHMGDTIWLSKTALVLDRINMYQTGDTIAAGAQFRELNGNESAVVEPQFLIY"
     gene            complement(2221..2841)
                     /locus_tag="PK742_04530"
     CDS             complement(2221..2841)
                     /locus_tag="PK742_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQU75889.1"
                     /translation="MLPSVKSCSSPRREDRINCQAPACLPLADKSGWLEESSKLCPDR
                     ETYCLIRSYCRCIDNSRPAPNASCRNFAKNLRLKRSYIILLFFMAVCIGVLASQLGNV
                     SSYAHFNDNKLLDGKEVRLKGTLAADQPVVYDPQVDPNSFTFYLVDSRGVEGKVVCYD
                     EKPRDFEKSDEIVLTGSLHDSVFYANDLLVKCPSKYVDEEIEKKEI"
     gene            2753..4156
                     /locus_tag="PK742_04535"
     CDS             2753..4156
                     /locus_tag="PK742_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQU75890.1"
                     /translation="MSAKGKQAGAWQLILSSLRGEEHDFTEGSIHRAVILLAIPMILE
                     MMMESVFAVVDIFFVNRLGPDATSVVGLTESVITLVYSMGIGLSAAATAMVARRTGEK
                     NRAAAAEAGKQAVFIGILVTLLVSVPGVLFSRTILSWMGAEPQAIDNGVGYTRTLFGS
                     SLFILLLFLINGIFRGAGNAAIAMKSLWLANIINIILDPLLIFGYGPFPELGLQGAAI
                     ATTIGRATGVVYQLYHLRKGQLHIRYLPIRMQWPIVKGLLAIAWPATFQFIIASASWI
                     ILAAMVATFGSEASAGYQTAIRILIFFILPAWGLSNAAATLVGQNLGAGSPQRAEKSV
                     VVTATYNVIFMAVVTVFFLLFSPPVIRFFIPDTGSDQFSYALDALQIVSAGYIFYGIG
                     MVVTQAFNGAGDSRTPTVINFVCFWLLQVPLAWYFLQKGWFGVHGVFAAIPVAETLIA
                     VISLILFRRGKWKATAV"
     gene            4229..6502
                     /locus_tag="PK742_04540"
     CDS             4229..6502
                     /locus_tag="PK742_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU75891.1"
                     /translation="MKRQILGLAGLLMLAVAAFGQQGDIRGFVYDIESGEPVIFANVV
                     IAGTTTGSATDINGFFSIPQVDAGSFQLMVTSIEYDTLIEPVTVTANQITNVKLYARE
                     ASRQLQTVTVTAQTQAKLTEVRISTIPITPKQIERLPSIGGEPDLAQYLQVLPGVIFT
                     GDQGGQLYIRGGSPIQNKVLLDGMVVYNPFHSIGLFSVFETDIIRSVDVYTGGFSAEY
                     GDRVSAVIDITTRDGNKKEFGGEVSVSPFMARTLLEGPIKKLDESGSSVSYLLTAKHS
                     YLDKTSTALYPYINGGDGIPYQFTDLYGKVSVNSNTGNKINFFGFNFRDSVSFSDVTT
                     FNWNSTGAGANFVVVPAESNVLIEGIFAYSNYAVTQNEADERPRNSLISGFNGGVDFT
                     YYIPDGDVKYGVEVVGFQTDYNFYNRLGLRYQQQQYTTQLSAFVKYRKVISKRLVIDP
                     SVRIQYYASLGEISPEPRLGLKYNLTSRIRLKFAGGLYSQNLISTRSDRDVVNLFSGF
                     LSGPESILATPDGEDATTKLQKATHAIGGIEFDVTDYIEFNVEPYIKYFNQLIEINRN
                     KIVPQDPDFATETGKAYGIDFLLKYDYKRLYIWLGYSLGYINRYDGEQTYPTHYDRRH
                     NMNVVTTYTFGEDQSWEVSGRWNLGSGFPFTQTAGFYESIDFLDGINTDYTTTNGNLG
                     IIYADDLNGGRLPYYHRLDVSVRKWKEFASGNKLNIVGSVTNVYNRENIFYFDRIRYE
                     RVDQLPILPSLSVSYTF"
     gene            6590..10075
                     /locus_tag="PK742_04545"
     CDS             6590..10075
                     /locus_tag="PK742_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU75892.1"
                     /translation="MRRWCVLCYAIFAGLTQTASAQFLDNDYELYGNLNQAALGSSVF
                     TAGDINGDGLSDWLAAEPDYKNGSYNKEGRVIIGLSQPDAPPVLFYLTAPVHQSLQYF
                     GSSVSTACDVNADGYSDILIGAKGYSNGETNEGAIFVFYGSVSGPDTTVFWMYESNSE
                     TASLGTSVSGPGDVNGDGYSDILAGAPLYESTPGKKNEGRIYLFYGSAEGVQTAGFWK
                     IRGGNANAKLGADVSGAGDFNADGYADLVAGMPGKSSLFASACGGARVYAGGPSGPVL
                     TATLYSYESFSEFGGSVGFAGDVNGNGYSDIIIGAPKMDNGMLLNAGAVFLYEGAGLN
                     DFSIPSSTFGIYAESHLGTDVSGLGDFNGDGYGDFAVGVPDYGFGGESEGRVLIYTGN
                     QDFSLFFHVPDFFYYSGIEGARVGASVGAAGDVNGDGLSDFITGAPELSLDLTGQGGV
                     FYVLGKRDERISGSRSLYPNQQLLHPCPDAQFGGSIANAGDVNGDGYHDLLVGAIKYT
                     CDDAEGGAAYLFYGGPDGFSDEPDWGVHCDQPFAKLGVSVSTAGDINNDGYADLMIAG
                     MEYDPGGLVVVYYGGPTGPSTDAPVAGDPAVSIHPDWFMTGEVGQSNLGSGLASAGDV
                     NGDGFNDIILGAYTWDNNIYTIPKPGRAYLIYGSASGLPEELDTTNSWIFTGTLDKND
                     NVGRTTSGAGDINGDGYSDIIIGAPRYENGQLHEGAFLVFYGGPDGITTDTPDYICEK
                     NSPQSNFGTNVSGAGDINGDGYSDIIIGSPLAENDSLGFEKAGALYAFYGSPDGLNPT
                     DEFSPPPADFAFYGTEANIQLGHIVNNIGDINGDGYSDVAVGSSHSNGDKGKVIIFFG
                     GKNGLSLQNKWSLGGEQDEEEFAWGIAGGDFNGDGLADLAVGSQNYDDTYTDEGSVFI
                     FHSQQNPHGGLTLQHAPESGSFSLPANSTVSGDQFVIGHSATSAIGMGNVRLRWEIVS
                     GNGSFTGLADSTASYSPAWNEVSDEWYTIDEGSAASSLFPGVYIEQPIQADLFCEERI
                     KWRVSLNFDPVQMIDGRPNAPWYYGSLQEDNEQGLWIGCYEEREAHANTTETEQQLFC
                     WPNPTTEYLYVLPDPHSSSIEVFDLQGHMVLQQMTDGTGICQLDLSLVASGSYLVVKR
                     DNRSLATSTAMIIRQ"
     gene            complement(10076..10783)
                     /locus_tag="PK742_04550"
     CDS             complement(10076..10783)
                     /locus_tag="PK742_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75893.1"
                     /translation="MMKRILLFPVLFVSLAALSQSADDLLNEYYKAINQEKLCQVTSF
                     IMTGNLTQMGVDIPMEVLQERPGKIRTSGTYMGMQFVQTYNGQEGWSLNPFMGQTEPV
                     AITGNELTSLHRQADLDGMLYDYAAKGSHVSMAQPEMIDMVSHPKIELTTEDTVVYEV
                     IFDPETFYCKLIRSRSDVQGRMSVVESVFSDFRTIDGIVFATSVETRIDGMTALRIHF
                     DEIKLNEDIDDSLFDKP"
     gene            10824..11441
                     /locus_tag="PK742_04555"
     CDS             10824..11441
                     /locus_tag="PK742_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HQU75894.1"
                     /translation="MDILFRQLLTGLILAVSSITGVAQQNDHDSWKQWYTEGFLEDEN
                     SPLDSAGITFLQFYPYDTAWVVEADFSYSSDGSVFIMPTSNGRQKEYVRYGTATFVLN
                     GISMQLTVFQSLKLREIPEYADYVFIPFNDDTNGTETYGGGRYLDLRFADVEDHKLTI
                     DFNRSYNPYCAYSDGYSCPVPPNENRLPVKVQAGEMLYGGMVPEH"
     gene            complement(11438..11860)
                     /locus_tag="PK742_04560"
     CDS             complement(11438..11860)
                     /locus_tag="PK742_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75895.1"
                     /translation="MKKLIVICLLAMGLSASAQQDGLGLGVIIGEPTGLSVKKFVGDD
                     AFSAAAAWSVKYDYFYVHLDYLKHNYFLGDQLPVYIGLGGALGFGKDMAVTARVPLGI
                     SYLFRGPFDVFFEVVPGLRIIPETDFDVMGAGGFHFYF"
     gene            complement(11944..>13189)
                     /locus_tag="PK742_04565"
     CDS             complement(11944..>13189)
                     /locus_tag="PK742_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU75896.1"
                     /translation="LPGADLIEPFKLFPEATLELPNKGTRVDLRPYADELEGLIGDIW
                     IGFSSPDGAAWVSQSTPGVALRTYTEFFGTWAEYYSDFHFRAITGPYSGAPNADFTFD
                     ASGDPSVLFTDLSTNYPDSWEWDFGDGVGSSTLQNPSYTYTANGDYEVCLTAGNAIGT
                     STHCETVTIGSFTPPAALFTFDDSADPAISFTDLSTNAPTAWVWTFGDGGGSTVADPS
                     HTYSTNGTFNVCLTASNLAGDDTYCTDVVIEGNAVAPVADYTYSGVGLTVNFTDISTN
                     TPTFWGWDFGDGTSSFDQNPVHSFGNSGVFEVCLTAGNMAGTDESCKTLSVATDLEDL
                     AAAGVVVYPNPATDQVLVNLSDWGGNATLQLYDLQGKLVLQQMMVPGTQVINLTELSS
                     GAYRLWISSVQKTATGVLLVDN"
BASE COUNT         3449 a         3235 c         3205 g         3300 t
ORIGIN      
        1 gtatatcagg aattgcggct ccactactgc tgattcattc ccgttgagtt cacgaaactg
       61 agccccggcc gcaatggtat ctcctgtctg atacatattg atcctgtcca gcaccaaggc
      121 ggtcttgctc agccagatcg tatctcccat atgaatggtg tgatccttct ctatcacatc
      181 ctgggccggc tggttcctgg ctggtatgga agatacatgt gtgaatacat catgtgtcag
      241 ataatgcttg gtgtcagggt tgggattgag ctgctgcgat tttttatctg tcagcagata
      301 aggatacaat atgaatgaag aagagacctc gccggtcttg tcatccaatt tttcatatct
      361 gaccttataa aatgtctttc tgttgtcagt actatcgcct gcataggtaa ccagatactg
      421 gcccatgacc acaggagagt ctttgtacaa aagaatattg tcacgcttga actcctgatc
      481 gaatgaatca ccataattga tgcccatggt attcttcgaa atgacctgct gactggcatt
      541 ggaaatcagg ataccaagca agagaatgcc aaagccaaca tgcgaaaccg aaccacccga
      601 tacacgcacc cttcccttca ttactgtgat gaagtactgc aggttggcca ggactgtaaa
      661 ggtggacata aacaacagca gcacccaggg gtcaggtaat ggaatattgc ctgaccgaag
      721 gaacaatgga tactccaggg ctcttgcaga aaagatggta acaggaatgg tcaagccgaa
      781 agaaatgatc agcgaacgcc agaatttctt catatctgac tgcctgtatc gcagaaactg
      841 cccgaaggcc gtcagcaccg caatgatgat ggctacccat atctgcacat tggtataata
      901 atggaccgga tctaccggtg ccgtgaaatc tgtcttgata gcagttccaa tgatcctgtt
      961 gatccaggcg aacaggtgat tgaaagcggg aatactggta aagaagatga catgcagtgc
     1021 tgagaataac agcaccagag atccgatgaa catccagaat tctctggaag cagttgcttc
     1081 ttccttcttc tctgcaggga tcttcttcca gtggcgaatg ataagccaac aggaaatgaa
     1141 cgtaaacaca aacaaataca acaacaggtg attctccagt ccgctaacca cgaaggaatg
     1201 cacgctggta tcgcccagga tgccagacct ggtcaggaaa cttgcataca acacaaagaa
     1261 aaaggacaaa gaaaccagaa tgaagttggc acgcagtgca tgaccggttg actttgaaac
     1321 cagcatagta tgcaaagcag caacaatgac cagccaggga atgaggctgg cattctctac
     1381 cggatcccat gcccagaaac caccgaagct cagtgcttca taggcccatg ctcctcccat
     1441 caggataccg gtaccaagca ccatcccgga gaacagacta tatggaatag ccggtgctat
     1501 ccaatctttg tatctgccag accacaaacc ggccatggca tatacaaaag ggacagtgat
     1561 actggcaaaa cccagaaaca acaccggcgg gtggatcacc atccagtaat tctgcaacaa
     1621 ggcgttcagg cctgtcccgt cctggatgaa ctgcatataa tcgctgaaaa ggaaaacagg
     1681 atcctgagtc agtgcatcct ccagcagcaa aaatggtgaa gagccaatct tcttaccgaa
     1741 cacataaaca ccagtaagaa atgacaacag aaatacctgc tgcaaagaca ggatggacat
     1801 cgttcttcct tcccattcgc ccgacctgcg catcagcaca agccccagca ccatgaccca
     1861 gaaggaccaa agcagaaagc tgccctcctg cccttcccag aaacaggaca ggatatattt
     1921 cattggcaga tcgcgactgg aatgctgcca gacatactgg tattcaaaat ggtggccgaa
     1981 gatcatgaag aacagcacta gcaccatacc tgcaatagaa gcggcatggg tcagaaaacc
     2041 ccagcgggcc agccttcgcc attcgaggct gcttgtcaga tccttttcac gaacggaaag
     2101 ccagtaacat actgccgacc atgcagcacc aaccaaagca agcagcacca gaaaatgtcc
     2161 ggcttgtccc atccagggat tgatcgcctg aactacgggg gattgtatct cctccatgcg
     2221 tcagatctct tttttttcga tctcctcgtc cacgtatttt gatggacatt tcaccaacag
     2281 atcattggca taaaaaacac tgtcatgcaa tgaaccggtc agcacgatct catcactttt
     2341 ctcaaaatca cgcggtttct catcataaca gaccaccttc ccctccacac ccctggaatc
     2401 taccagataa aatgtgaatg aattcgggtc cacctgcgga tcatatacca ctggctggtc
     2461 tgccgccagc gtgcctttca gcctaacttc tttgccatca agcaacttgt tgtcgttgaa
     2521 atgtgcatag gagcttacat tgcccaactg agatgccagt acaccaatgc agactgccat
     2581 gaaaaaaagg agtatgatgt aggagcgttt caaccttaaa ttttttgcaa aattacgaca
     2641 cgaagcatta ggagccggtc ttgaattgtc aatacatcta caataactcc tgatcagaca
     2701 ataagtttca cgatcaggac ataattttga gctctcctct aaccaaccag atttgtcagc
     2761 caaaggtaaa caggcgggag cctggcagtt gatcctgtct tccctgcggg gggaagagca
     2821 tgattttacc gaaggaagca tccatcgggc ggtcatttta ctggccattc cgatgatcct
     2881 ggagatgatg atggaaagtg tctttgccgt ggttgatatc ttttttgtca atcgcctggg
     2941 tcctgacgca acctccgtgg tcggattgac agagagtgtc atcaccctgg tctattccat
     3001 gggcatcgga cttagtgcgg ctgccactgc catggtggcc cgtcgcacag gtgaaaagaa
     3061 ccgggcagcc gctgctgaag cgggcaaaca agcggtattc atcggaatcc tggtaacatt
     3121 gctggtgtct gttcccggtg tactattctc caggaccatc ctctcctgga tgggagccga
     3181 gccccaggcc atagacaacg gggtcggtta tacgagaacc ctcttcggca gcagcctctt
     3241 cattctgtta ctgttcctga tcaatggcat ttttcgtggt gccggaaatg ctgccatagc
     3301 gatgaagagt ttgtggctgg ccaacatcat caacatcatc ctggacccat tgctgatatt
     3361 cggttatggt ccttttccgg aacttggcct gcagggggcg gctatagcca caaccatcgg
     3421 cagggccacc ggtgtggtgt accagctata ccacctgcgc aagggacagt tgcatatcag
     3481 atacctaccc atacgtatgc aatggccaat tgtaaaagga cttcttgcca tcgcatggcc
     3541 tgctactttt cagttcatca ttgcatctgc cagctggatc attctggctg ccatggtggc
     3601 aacgttcggc agtgaggcaa gtgcaggtta tcagacagcc atccgcatcc tgatcttttt
     3661 tattctgccg gcttgggggc tgagcaatgc agcggccaca ctggttggac aaaacctggg
     3721 agcaggatct ccgcaaaggg cagaaaaaag tgtggtagta acagctactt acaatgtcat
     3781 cttcatggca gtggtaaccg ttttttttct gttgttcagc ccaccggtca tccgtttttt
     3841 tattcctgac accggtagtg atcaattcag ttatgcactg gatgccctgc agattgtcag
     3901 cgcaggttat atattctatg gtatcgggat ggtggtgacc caggcattca acggagccgg
     3961 cgacagccgc accccaacag tcatcaattt tgtatgcttc tggctgcttc aggtaccgct
     4021 tgcctggtat ttccttcaaa agggatggtt tggagtacat ggtgtgtttg ctgccatacc
     4081 cgttgcagaa accctgatag ccgtgatcag cctgatcctg ttcagacggg gcaaatggaa
     4141 ggcaactgcc gtttaggggt caaatagtgg tcatattact gcaatggctg aagttggtag
     4201 tgattattga gtattttcgc aaccgtttat gaagagacag atattgggtt tggcaggtct
     4261 gctgatgttg gccgtagctg ctttcgggca acagggagac attcgtggtt ttgtgtatga
     4321 tattgaatcg ggtgaacccg tcatttttgc caacgtggtc attgcaggca ccactacagg
     4381 gtctgcaacc gatatcaatg gtttcttttc tattccgcag gttgatgcag gttcatttca
     4441 gctgatggta accagtatcg aatatgatac actgatcgaa cctgtaactg ttactgccaa
     4501 tcagatcacc aatgtcaagc tgtatgcccg ggaggcttcc cgccagctgc aaaccgtaac
     4561 ggttacagcg caaacacagg ccaagctgac agaggtgcgc atttctacca tacccattac
     4621 gcctaagcag atcgaaagac tgccttccat aggtggtgaa cctgaccttg cccagtatct
     4681 gcaggtattg ccgggtgtca tctttaccgg agatcagggc ggacagttat atatccgtgg
     4741 tggatcaccc attcagaaca aggtattact ggacggcatg gtagtgtaca atcctttcca
     4801 ctccattgga ctgttctctg tttttgagac agacatcatc cgaagtgtag atgtctatac
     4861 aggtggtttc agtgctgaat acggcgaccg tgtgtctgca gtcattgata tcaccacccg
     4921 cgatggaaat aagaaagaat ttggcggtga agtaagtgtg agcccgttca tggcaagaac
     4981 cctgctggaa ggccccatca aaaaactgga cgaaagcgga agcagcgtga gttatctcct
     5041 cacagccaag cactcgtatc tggataaaac gtcaactgcc ctgtatccct atataaatgg
     5101 tggcgatggt atcccctatc agttcactga tctgtatggc aaggtatctg tcaattcaaa
     5161 caccggaaac aagatcaatt tttttggctt caattttcgc gacagcgtct ctttctcaga
     5221 tgtgaccact ttcaactgga acagtaccgg tgcgggtgcc aacttcgtgg tagtgcctgc
     5281 tgaaagcaat gtgctgattg aagggatttt tgcttattcc aattacgcag taacccagaa
     5341 tgaagcagac gagagaccac gcaacagcct catcagcgga ttcaacggtg gtgttgactt
     5401 tacctattac attcctgatg gtgatgtaaa atacggtgta gaagtagtag gctttcagac
     5461 agactataac ttctacaacc gcctcgggct tcgttatcag cagcaacaat acaccaccca
     5521 gcttagtgcc tttgtgaaat acagaaaggt catcagcaag cggctggtca ttgatcccag
     5581 tgtacgtatc cagtattacg ccagcctggg tgagatatca cctgaaccca gactaggcct
     5641 gaagtacaac cttaccagcc gcatcaggct aaaatttgcc ggaggacttt attctcagaa
     5701 cctcatttca acccgaagtg accgtgatgt agtgaacctg ttttcaggtt tcctgagcgg
     5761 gccggaaagt attctggcaa ctcctgatgg agaagacgcc accaccaagc tgcaaaaagc
     5821 tacccatgca attggcggaa ttgaatttga tgtaacagac tatattgaat tcaatgtgga
     5881 gccgtatatc aagtatttca accagctgat cgagatcaac cgcaacaaga tcgttccgca
     5941 ggatcctgac tttgccacag agaccggaaa agcttatggt atagacttcc tgctgaagta
     6001 cgattataag cgtctgtata tctggcttgg ctactcactc ggatacatca atcggtatga
     6061 tggtgagcag acctatccaa cccattatga ccgtcgccat aacatgaacg tggttaccac
     6121 ttacaccttc ggtgaagacc agagctggga agttagcggt cgctggaatc ttggttcagg
     6181 tttccctttc acccagactg cagggtttta cgaaagcatc gactttctgg acggcatcaa
     6241 tacagattat accaccacca acggaaacct gggcatcata tatgctgatg atctgaacgg
     6301 tggccggtta ccatactacc accgcctgga tgtgtctgta cgaaagtgga aggagttcgc
     6361 ttccggcaat aaactgaata ttgtcgggtc agttacgaac gtttataaca gagaaaacat
     6421 cttctatttt gaccgcatcc gctacgaacg tgtagaccag ctgcccatcc ttcccagttt
     6481 atcggtcagt tatacttttt aattctggta agaaataaat ttaatatact tttgtgacgg
     6541 cactctttcc ccgtgctgca cacaaatgaa agcaacctat acgcacttct tgaggaggtg
     6601 gtgcgttctg tgttatgcta tttttgcagg cttaacacaa actgcctcag ctcaatttct
     6661 ggacaatgac tatgaacttt acggcaacct aaaccaggca gccctgggaa gttctgtgtt
     6721 cacggcagga gacattaacg gcgacggact ctcagactgg cttgccgccg aacctgacta
     6781 caagaacgga tcctataaca aagaaggaag ggtcatcatt ggtctcagcc agccggatgc
     6841 tccgccggta ttgttctacc ttacagctcc ggttcaccaa agcctgcagt attttggctc
     6901 ctctgtttcc actgcctgcg acgtgaatgc cgatggttac agtgacattc ttattggtgc
     6961 caaaggatac agcaatggcg aaaccaatga gggcgccata tttgtcttct acggttctgt
     7021 atccggccct gataccaccg tattctggat gtatgaaagc aacagcgaga cagcttctct
     7081 gggcaccagt gtttcgggcc ccggcgatgt gaacggagat ggatacagcg atattctggc
     7141 aggtgccccc ctgtatgaat ctaccccggg caagaagaac gaaggacgta tctatctgtt
     7201 ttacggttct gctgaaggtg tgcaaacagc cggtttctgg aagatccggg gtggaaatgc
     7261 aaacgctaaa ctgggcgccg atgttagcgg tgctggcgat tttaacgcag atggttatgc
     7321 tgatctggtg gcaggcatgc ccggcaaaag ctctctgttt gcttctgcct gcggtggtgc
     7381 aagggtctat gccggaggac cttccggccc tgtgctgacg gccaccctct acagctatga
     7441 aagcttttca gaatttggag gatcagtggg gtttgccggc gatgtgaacg gaaacggata
     7501 ctcagacatc atcataggtg ctccgaaaat ggacaatggt atgttgctga atgccggtgc
     7561 tgtattcctg tatgaaggtg caggcctgaa cgatttctct atacccagca gtacattcgg
     7621 tatctatgct gaatctcacc tcggaacgga cgtgagcgga ttgggtgatt ttaatggcga
     7681 tggatacggc gattttgccg taggtgtgcc cgactatgga tttggtggag aaagtgaagg
     7741 acgtgtgctt atttataccg gcaaccagga cttttccctt ttcttccatg ttcctgactt
     7801 cttctattat tcgggtattg aaggcgctcg tgttggcgct tccgttggcg ctgccggcga
     7861 tgtgaacggc gacgggctgt cagattttat tacaggagct cccgaactat cgctggacct
     7921 gaccggccag ggtggtgtct tctatgtgtt gggaaaaaga gatgaacgca tctccggctc
     7981 cagatcatta taccccaatc agcagctgct gcacccctgc cctgatgctc agtttggtgg
     8041 cagtatagcc aatgccggcg atgtgaatgg cgatggctac catgacctcc tggtgggagc
     8101 gatcaaatat acctgcgatg atgcagaagg cggagcagct tacctgttct acggtggccc
     8161 cgatggcttt tccgatgaac ctgactgggg tgttcactgc gatcagcctt tcgccaagct
     8221 tggagtttct gttagcacag cgggcgatat caacaacgat ggatatgctg atcttatgat
     8281 agcaggtatg gaatatgacc cgggtggact tgtagtggtg tactatggcg gcccgacagg
     8341 cccttccacc gatgcgcctg tggccggcga tcctgcagtt tccattcacc ctgactggtt
     8401 catgactggt gaagtaggtc agtccaacct gggcagcggg cttgcttctg ccggcgatgt
     8461 gaacggcgac ggtttcaatg acatcattct cggagcttat acctgggaca acaacatcta
     8521 taccattcca aaaccaggaa gagcttacct gatctatggc tcagccagtg ggttacctga
     8581 agaacttgac accaccaatt catggatttt tacaggcacc cttgataaaa atgacaacgt
     8641 aggcagaacc acatccggtg caggcgatat taatggtgat ggctattctg atatcatcat
     8701 tggggcaccc aggtacgaaa acggccagct gcacgaaggt gcatttctgg tattctatgg
     8761 aggccccgat ggcataacca cagatacccc tgattatata tgtgaaaaaa attcacctca
     8821 aagtaatttc ggaaccaacg taagcggagc aggcgatatc aacggcgatg gctattctga
     8881 catcatcatt ggatcgccgc tggcagaaaa tgattctctg ggatttgaaa aagccggtgc
     8941 actgtatgcc ttttacggat cgccggatgg gctcaatcca accgacgaat tctcgccccc
     9001 tcctgccgat tttgcattct atggaaccga ggccaatatt cagctgggac atatcgtgaa
     9061 caatattggc gatatcaacg gtgatggcta ctcagatgtg gcagttggct ccagccattc
     9121 caatggagat aaaggaaagg tcatcatttt ctttggcggt aaaaacggtt tgtcgctgca
     9181 aaacaaatgg tcattaggtg gagaacagga tgaggaagaa tttgcctggg gcattgccgg
     9241 tggcgatttt aacggagatg gcctggctga tctggccgta ggctctcaga actatgacga
     9301 tacctataca gatgaaggaa gtgtcttcat ttttcatagt cagcagaacc cgcatggcgg
     9361 acttaccttg cagcacgctc cggagtcagg cagcttttcg cttcctgcca attccactgt
     9421 atcaggcgat cagtttgtga tcggtcactc tgctacctca gctattggaa tggggaatgt
     9481 caggctgcga tgggaaatag tgagtggcaa cggatccttt acaggattgg ctgacagtac
     9541 cgccagctac agccctgcct ggaatgaagt tagtgatgag tggtacacca tagacgaagg
     9601 atcagctgct tcaagtcttt tccccggggt atatattgaa caacctattc aggcagacct
     9661 gttctgtgaa gagcgcatca aatggcgggt gtctctgaat tttgatccgg tgcagatgat
     9721 agacggcaga cccaatgcac cctggtatta tggaagcctg caggaagata atgaacaggg
     9781 tttatggatt ggctgctatg aggaaagaga agcacatgcc aacaccaccg aaacagaaca
     9841 acagctgttt tgctggccaa accctactac cgaatatttg tatgtgctac ctgaccctca
     9901 ttccagcagc attgaggtat ttgatctgca gggtcatatg gttctgcaac aaatgactga
     9961 tggtacgggt atctgtcaac ttgacctcag tcttgtggct tccggctcct atctggtggt
    10021 caaacgtgat aacagaagtc tggctacctc cactgctatg ataatcagac agtgatcagg
    10081 gtttgtcgaa caggctgtcg tcaatatctt cattcagttt gatctcatca aaatgaatgc
    10141 gtaaagcggt catgccatca attctagtct ccactgacgt tgcaaatact attccatcga
    10201 tcgtgcggaa atcagaaaag acagattcta caacagacat ccttccctga acatcagacc
    10261 gtgaccggat caatttgcag taaaaagttt ccggatcaaa aatgacctca tagaccacag
    10321 tatcttcggt ggtcagttct atcttaggat gagacaccat gtctatcatt tctggctgtg
    10381 ccatgctgac atgacttcct ttagctgcat agtcatacag cataccgtcc aggtctgcct
    10441 gccggtgtag tgatgtcagc tcatttccgg tgatggctac aggttcggtt tgccccatga
    10501 atggattcag gctccagccc tcctgaccat tatacgtctg cacaaactgc atgcccatat
    10561 aggtcccgga tgtccggatc ttcccgggtc tttcctgcag cacctccatg ggaatatcca
    10621 cacccatttg ggtcaggttg ccggtcatta tgaatgaggt tacctggcac agtttctcct
    10681 ggttgatggc cttgtaatat tcgttcagca ggtcatctgc cgactggctt aaggcagcaa
    10741 gagaaacaaa caaaaccggg aacagcagga tgcgtttcat catgattctt tgccacaaag
    10801 ttaattcagg atagcttcgc cgtatggata tcttatttcg ccagctccta accggtctta
    10861 ttctggcagt ttcttctata acaggagtag cacagcagaa tgatcatgac tcctggaagc
    10921 aatggtacac agaaggcttt ctggaagatg agaacagccc tctggacagt gccggaatca
    10981 cctttctgca attttatccc tatgatacgg cgtgggtggt agaggcagat ttttcttatt
    11041 catcagatgg atcggtattc atcatgccca cctcgaacgg caggcaaaaa gaatatgtaa
    11101 gatatggcac tgctacattt gtgctgaatg gtatcagcat gcagttaacg gtgtttcaaa
    11161 gtctgaaact tagggagata ccggagtatg cagattatgt gttcattcca ttcaacgatg
    11221 ataccaacgg cacagagacc tatggcggcg gacgctacct ggatctgcga tttgccgatg
    11281 tagaagacca taaactgacc attgatttca acagaagcta caatccgtac tgcgcctatt
    11341 cagatggcta ctcctgccct gttcctccca atgaaaatcg cctgcccgta aaggtacagg
    11401 caggcgaaat gttgtatggc ggaatggtgc cggagcacta aaagtaaaag tgaaaacctc
    11461 cggctcccat cacatcgaaa tctgtttcag gtatgatcct taatccgggc accacctcaa
    11521 agaagacatc gaacggccct ctgaacaggt agctgatacc aagaggcact ctggctgtaa
    11581 cagccatatc tttcccaaaa cccagagcac caccaagacc gatgtaaacc ggcagctgat
    11641 ctcccagaaa gtaattgtgt ttcaggtagt ccaggtggac gtagaaataa tcatacttga
    11701 ccgaccaggc agcagctgct gagaaggcat cgtcaccaac aaattttttc actgaaaggc
    11761 ccgtgggttc tccaatgatc acacccagtc caagtccatc ctgctgtgct gaggcagaca
    11821 gacccatggc cagtaaacaa atcacgatca gtttcttcat gtattgattt ttttgtttcg
    11881 gtagtagcag gttgcagtct gctactaccg tacaaatatc atacctctat gcatgaaaag
    11941 atctcagtta tccaccagga gaacgccggt tgccgtcttt tgcacggaac tgatccaaag
    12001 ccggtaagct cctgaagaca gttcggtcag gttgataacc tgcgtacctg gaaccatcat
    12061 ttgctgcaat accagtttgc cctgcaggtc atacaattgc agtgtggcat tacctcccca
    12121 atctgagaga ttcaccaaca cctgatctgt ggcaggatta ggataaacga caacacctgc
    12181 agctgccaga tcttccagat cagttgccac actcagtgtt ttgcaggatt catcagttcc
    12241 ggccatattg cctgcagtca gacagacttc aaatacgccg ctgtttccga atgaatgaac
    12301 aggattctga tcgaaagagg aagttccgtc gccgaaatcc catccccaga aagtaggggt
    12361 attggtgctg atatctgtga agttcactgt caatcccaca ccactgtagg tatagtcggc
    12421 aaccggtgca actgcatttc cttcaatcac cacatcagta cagtatgtat catcgcctgc
    12481 aagattggaa gccgtcaaac aaacattgaa agtaccattg gtgctatatg tatgtgacgg
    12541 atcagcaacc gtgcttcccc ctccatcgcc gaaggtccac acccatgctg ttggtgcatt
    12601 ggtgctcagg tctgtgaagg aaatggcagg atctgcgctg tcatcaaatg tgaataatgc
    12661 agcaggtggc gtaaagcttc ctatcgtaac cgtttcacaa tgggtagagg taccaatggc
    12721 atttcctgcg gtcagacaga cctcataatc accattggct gtataagtat aagatggatt
    12781 ctgcagggta ctgcttccca cgccatcacc gaaatcccat tcccagctat caggataatt
    12841 ggtactcagg tcggtgaata ggaccgaagg atcgccgctg gcatcgaatg taaagtcggc
    12901 gttgggagca ccgctgtatg gacctgtaat ggctctgaaa tgaaaatcag aatagtactc
    12961 agcccaggtg ccaaagaact ccgtataggt tcttagagca actcccgggg tagattgtga
    13021 tacccaggct gcaccatcag gcgaactgaa cccgatccag atatcgccaa tcagtccttc
    13081 cagttcatcg gcatacggac gaagatctac acgggtgcct ttattgggca attcaagtgt
    13141 ggcttcagga aagagtttaa agggttcgat gaggtcagct cccggcagg
//
LOCUS       DAOMAX010000054        12994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_594_length_12994_cov_4.499575, whole genome shotgun sequence.
ACCESSION   DAOMAX010000054 DAOMAX010000000
VERSION     DAOMAX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_594_length_12994_cov_4.499575"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK742_04570"
     CDS             <1..761
                     /locus_tag="PK742_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HQU75897.1"
                     /translation="TEHRGHATEITERAIKDGVEVVAVAGGDGSVNEVAMALRGTSVK
                     LAILPYGSGNGLARALGNPMKIAEAVRLINTGKVRAVDTAKVNDHVFFSLIGIGFDAF
                     AVKVFDREEARGFLAYAWAAIRSFSAYQSYSFVMDCDGVEYNGAAFLINICNSDQYGY
                     NVRIAPFAEPDDGLLDVVLVRDLPKWKAVWLVFLVLMNKHHNSRMLEFIKARKVHLQT
                     SRHAYMQVDGETMHKDKQFDVQICNEQLQVLVKP"
     gene            771..1115
                     /locus_tag="PK742_04575"
     CDS             771..1115
                     /locus_tag="PK742_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HQU75898.1"
                     /translation="MSNRNKGKQGVVYSTDPDFQFREEDAQESDTLLPQQQNLKVYRD
                     KKQRGGKEVTIIEGFIGRTEDLEKLGKDLKNKCGTGGSVKDGLILVQGDQRNKVGDYL
                     TSKGYKFKFAGG"
     gene            complement(1112..1447)
                     /locus_tag="PK742_04580"
     CDS             complement(1112..1447)
                     /locus_tag="PK742_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HQU75899.1"
                     /translation="MAEITFDTFLQTDMRTGTILEAVVFENARKPAYQLTIDFGSLGI
                     KRSSAQITDHYTPESLIGQQVIAVVNFPPKQIANFFSECLVLGSVDADGKVVLLQPER
                     KVENGLRIA"
     gene            1716..4799
                     /locus_tag="PK742_04585"
     CDS             1716..4799
                     /locus_tag="PK742_04585"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU75900.1"
                     /translation="MCKPVWLLVLIFSSVTVYAQFNLAGDAISLGGDCYRLTTTLTSQ
                     EGAAWYTTPISLEETFKISFDIYTGTSDGGADGMTFTFQTNNTGVGIGGGSLGVQGIS
                     PSVHLEFDTWQNGDKGDPWYDHMGLNSMGEASHSLSTALTPYYYVETGVTNIEDNTYR
                     EVIVLWEADIQTYSVWVDCAYRFSYTGDLVNDIFGGDPEVYFGFTGSTGGAVNTHIVC
                     FNYIVYPEDPEPLPDAFICEGDSVQMEAAEGFVTYTWSPATGVSDPGIADPWFSPVTT
                     TTYYLTMSDECGISEYDTVLVEVEEDDSEFSYPADTYCAEGTVLPDYIESPGGFFSIE
                     PPGLLINSTTGQLNLDGATPGTYVVEYVPPAGICPVSSTQTITIEPFPDASFSYPETI
                     YCAAGTALPADVVVGGGSFSASPAGLSVNTSTGLIDLATATIGETYTIYYDVTVFCSS
                     ADSFTITIQDFEDAGFAYDETHYCPTGTVLPAFINTAGGTFSVSPAGLVVDPSTGELD
                     LSSGTVGTNYTITYLTSATDCGASSSVTLFIDPLDDASFAYSDDHYCATGTILPDYIT
                     TSGGSFTADPPSLLVDAVSGEVDLETGAVGTTYTITYTTADGPCQNWDAVSITIDAND
                     DSTFSYTDDTFCPEGVALPDLIATPGGSFTAWPAGLGVDAFSGLLDLSTATPGTYTVT
                     YTTPVSLCTSSMDLTITIDTVTDAFFAYDNPAYCRVGSIIPFVLHPGGGFTADAGLVI
                     DAETGEVDLESCTPGGPYAVYYQSPGCTEEDTVWLTIWPEPELSFAPDGPLCIEDAPL
                     LLTADPTGGSFAGTGVAGGSFVPGAAGEGLFELTYSYTDVNGCSNEISAFLEVIENSV
                     SVGADVTIIEGTSTALQAVGGVIFSWEPADGLSCTGCASPLASPAQTTTYWVTSWDAY
                     GCMAIDTVRVEVVPFDDVTVFVPNTFTPNFDGINDYLFVYGSDIERINYLRIFDRWGN
                     EVWFGENLPAGMENTGWNGTSNGTPVNSGTYAFIAEVQLTFGVSKRVSGNVTLLR"
     gene            4853..6652
                     /locus_tag="PK742_04590"
     CDS             4853..6652
                     /locus_tag="PK742_04590"
                     /inference="COORDINATES: protein motif:HMM:NF037833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU75901.1"
                     /translation="MKYCLILISVLLVQGARAQWVLNGDAEELGDNCFQLTEELPTQA
                     GSVWYDSLINLEEDFEVNFEIYLGTLDASGADGIAFVLQPVSTGLGESGGGLGYEGIE
                     PSLDIEFDTWQNGIYGDPTYDHISIMQDGILDHFAATALTPYTQILSGSTNAEDGNYH
                     QVRITWEAGSQTISCFVDCDLRVTYSGDIIDDIFDGDPFVFMGFTAATGGSYNDQIVC
                     LDYVTAVDELDDVTVCPGDSVQLFLPEGFVSYSWSPATGVSDPTAPDPWLSPDVTTTY
                     TVTITDECGNTITNIVTVTVGSPELVDLGPDLSLCEGDEELISVFLAGATYEWNNGST
                     LPAITITESGTYWVELTLGNCTDADTINVNYSPAPFVNFGPDTVVCGLSEVVFLDATN
                     PGATYLWQDGSTSPTYTVTEDGLYFVTVSIGSCIDQDSLAVNYSSYPAVDLGPDIYLC
                     PDSVVLLLAGPPVFVYTWQDGSSGNTYLITESGEYWVDVNSNGCITRAYIDVIDDPCI
                     CEMIVPNAFSPNNDGYNDEFRQLECSQLNRFNMKIYNRWGELMFETSDYSAGWDGTFR
                     GEQCEMGAYVFVIEYDIIDGESGIKQGSFLLLR"
     gene            6748..10320
                     /gene="dnaE"
                     /locus_tag="PK742_04595"
     CDS             6748..10320
                     /gene="dnaE"
                     /locus_tag="PK742_04595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQU75902.1"
                     /translation="MKFSHLHNHTQFSLLDGASPIKKLYSKAVEDGMPALAITDHGNM
                     FGVFHFVAEAAKHTNEDGSLKVKPIVGCEFYVVEDRHKRQFTREERDVRYHQVLLAKN
                     DVGYRNLVKLCSLGYMEGMYSKYPRIDRSLIEQYHEGLIATTCCLAAEVPRAIIRKGE
                     EEAEEVFKWWLDLFGDDYYIELQRHGIPEQDEVNAVLQKWSAKYNVPMIATNDSHYVD
                     RQDANAHDILLCINTAEKQATPKAKDFSDEEGSMRGTRFAFYNDEFFFKTQAEMGKVF
                     RDVPEALDNTNAIIDKVEVLDLKKDILLPHFQLPAGFTDQDAYLHHLTMEGAKARYKD
                     ITPDIQERLDFELFTIRTMGFAGYFLIVADFINHGKDIGVFVGPGRGSAAGSAVAYCI
                     GITNIDPIHYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIEYVVDKYGKNQVAQLIT
                     FGTMAAKMSIKDVGRVLDLPLAEANMLAKYVPERPGINLNRLMFAPLKGEGSLAEKEN
                     LSPDEMTNIQQLRKIMQGDDLQAKVIQEAVKLEGSVRNTGIHAAGIIIAPSDLTDLLP
                     VAVAKDSELLVTQYEGEIIESAGVIKMDFLGLKTLSILKTALKHIKLNHHRDIDLDIL
                     ALEDEPTFGLYQRGETNGTFQFESAGMQKYLKELKPDKFEDLIAMNALYRPGPMDYIP
                     TYIARKQGREKITYDLPEMEEILKETYGVTVYQEQVMLLSQRLANFTKGDADTLRKAM
                     GKKQKAVLDKMKSKFMEGCVANGHPDKVCEKIWTDWEAFAQYAFNKSHSTCYALVAYQ
                     TAYLKANYPAEYMCAVLTHNQNNLDKVSFFMDECRRMEIMVLGPDINESGVSFTVNEK
                     GEIRFGLAAIKGVGEAAVQEIIQQREQDGAFQTFFDLVRRVNLRTVNKKTMESLVLAG
                     AFDTFGLKRAQYFTQQKNDQLNVLEHAIKYGGTYQQGSQQEQATLFGDAVEVEIAEPT
                     VPDVPEWNSVDKLKQEYDVCGIYLSGHPLDEHRLEVEAFRTCSIADMHQFVGRTISLI
                     GLVGSSQTRTTKTGDKFSIFTLEDYSGNLECMLFKEMYVKFKHYVEDPGYIIHVKGNC
                     QPGYRDKEKIEVRVQEVELLSETRAKRSIKVRLKMPLARVDTKQIVDLKAILDAHPGN
                     DQLAMEVYHEQMQVPLRSVNGLVQVDKGLIKALGRFGEVQLAVG"
     gene            10402..10719
                     /gene="trxA"
                     /locus_tag="PK742_04600"
     CDS             10402..10719
                     /gene="trxA"
                     /locus_tag="PK742_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQU75903.1"
                     /translation="MATEFTDANFKEQVLDSDKLTVIDFWAVWCGPCRMVAPVIEELA
                     KDYDGQVNIGKVDVDSNPMVSQQFGIRNIPTILFVKNGEVVDKQVGAVPRSVLEAKMK
                     QHM"
     gene            10786..11721
                     /locus_tag="PK742_04605"
     CDS             10786..11721
                     /locus_tag="PK742_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQU75904.1"
                     /translation="MQTELLRLQDIRQVEHLELLAKQVVEGFILGLHKSPFHGFSVEF
                     AEHRIYNPGESTRYIDWKVFGRTDRLYSKKFEEETNLRCQLVIDTSSSMYFPEQPAPS
                     GKFTMNKVVFSAVCAAAIIQMLRGQRDAAGLTLFSDAVHLHTKAGSGHVHHQLLFSNL
                     EKFLGEKSRNNKTRAADCLHRIAEQIHRRSMVVIFSDMMEGGEDQDDLFSALQHLKYN
                     KHEVVLFHVVDHLKELEFEYENRPYVFVDMETGEKVRIQGNEIRTHYKERMQKYLNNL
                     KLRCHQYQIDFVEADINKDFKQVLMPYLVKRMKMK"
     gene            11725..12489
                     /locus_tag="PK742_04610"
     CDS             11725..12489
                     /locus_tag="PK742_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQU75905.1"
                     /translation="MAVLHFKSSGKGEPLIILHGLFGMLDNWQTIANRLDNRYLVYLV
                     DLRNHGRSPHMESMTYPEMAEDIVQLMDAEGIPAANILGHSMGGKVAMQLAFDHPLRV
                     QKLIVADIGPKGYPPGHDQIFEALDAVVLPAVTQRRDAEEMMQQVLHDPGIILFLMKN
                     LQRHTDGSYGWRMNLEVIREQYSSIRGPVSGESFHDPALFIRGSRSDYIPDNDWPAIQ
                     ELFPLAALVTIPDAGHWVHADQPDMLLNEVVRFLLS"
     gene            12518..>12994
                     /locus_tag="PK742_04615"
     CDS             12518..>12994
                     /locus_tag="PK742_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75906.1"
                     /translation="MKAIVLSVAVLVCAVGYTQPVITADVLPEVAESFSLTPFDATGF
                     DPGDTGAGVTWNFSDLDASGSVETTVAHDASTIGGHEDFPEANVAWTNDGLTFTFFKS
                     NSSVLENWGSVVNDADLGFISIPYSDGEELLTLPLNFGDTNEDDFDRAFSAGGFDVA"
BASE COUNT         3333 a         3022 c         3404 g         3235 t
ORIGIN      
        1 atacggaaca tcgtggtcat gccacagaga tcactgaaag ggcgataaaa gatggcgtgg
       61 aggtggtggc agttgccgga ggcgatggtt cggtcaatga ggtggccatg gctctccgcg
      121 gaacaagtgt gaagctcgct attctgcctt atggttccgg taacggactg gcacgtgcat
      181 tgggtaatcc gatgaagata gctgaggcag taaggctcat caataccggg aaagttcggg
      241 cagtagatac agcaaaagtg aatgatcatg tgttcttcag tctgatcggt attggctttg
      301 atgcgtttgc agtgaaggtg ttcgaccggg aagaagcccg cggattcctg gcctatgcct
      361 gggctgccat caggagtttt tctgcctatc agtcttattc ttttgtgatg gattgtgatg
      421 gagtggaata caatggtgcg gcatttctga tcaatatctg caacagcgac cagtacggat
      481 acaatgtgcg catagcacca ttcgctgagc ccgatgatgg tttgctggat gtggtgttgg
      541 taagagatct tcccaaatgg aaggcagttt ggctggtatt tctggtgctc atgaacaaac
      601 accataacag tcgtatgctg gagttcatca aagcccgcaa agtacacctg caaacttccc
      661 gtcatgccta tatgcaggtg gacggtgaga ccatgcacaa agacaagcag tttgacgtac
      721 agatctgcaa tgaacaatta caagtactgg tgaagcctta attttggaat atgtctaatc
      781 gcaacaaggg gaaacaaggt gtggtctatt ccaccgatcc ggattttcag ttccgggaag
      841 aagatgcaca ggagtctgat actttattgc cacagcaaca gaacctgaag gtctatcgcg
      901 acaagaaaca acggggcgga aaagaggtta ccatcattga gggattcatc ggccgtaccg
      961 aagacctgga gaaactgggt aaagatctca agaacaaatg cggtacgggt ggcagtgtga
     1021 aagacggact tattttagta cagggcgatc agcgcaacaa ggtgggcgac tacctgactt
     1081 caaagggata caaattcaaa ttcgccggag gctaagcgat tcgcaggcca ttctccactt
     1141 ttctttccgg ttgcagcaag accaccttac catctgcatc cacactgccc agcaccagac
     1201 attcagaaaa gaaattagcg atctgtttgg gcgggaagtt cactacagca atgacctgct
     1261 gcccgatgag cgattcaggt gtatagtgat cagtgatctg cgcagaagat cttttgatac
     1321 ccagtgatcc gaaatctatg gtcagctggt aagcgggttt cctggcattt tcaaatacta
     1381 ctgcttccag gatggtgccg gtacgcatat cggtttgcag gaaagtgtca aatgtgattt
     1441 cagccatgga tatagtttgg gcatgaatgt aagtaacaat agagttgaat gtttgcccat
     1501 aatgccggta cactcatttt ctgaaagatt ttctggcagg aatcttcttt attgtccgat
     1561 ttatcaggtt ctgtttctcc tcagaagccc ttttgcaagg tgttttgcag ttatcttata
     1621 tttaactgat ttgtcaggca agagacatct tatgctgatc cggaaagaat aaggcacttg
     1681 ttgtaaaaaa tacaataact ttatcgaccc aacccatgtg taaacctgtc tggctgttag
     1741 ttctcatctt tagttcggtt actgtgtatg cacagttcaa cctggctggt gatgccatca
     1801 gcctgggtgg tgactgctat cggttgacca caactctgac cagtcaggaa ggagccgcat
     1861 ggtacaccac acccatcagt ctggaggaaa ccttcaagat cagttttgac atttataccg
     1921 gaacctcaga cggaggtgct gatggtatga cattcacctt ccaaaccaat aatacaggag
     1981 taggtattgg tggaggtagt ctgggtgtgc agggcatcag tccgtcggtg cacctggagt
     2041 ttgatacctg gcagaatggc gacaaaggcg atccctggta tgaccacatg ggtctgaaca
     2101 gcatgggcga agcaagtcat agtttgtcaa ctgcactaac gccttactac tatgtagaaa
     2161 cgggggtcac caacatagaa gacaatacct accgtgaggt gatcgtattg tgggaggccg
     2221 atattcagac ctactccgtt tgggtggact gtgcgtatag attttcttac accggcgatc
     2281 tggtcaacga catctttggt ggtgatcctg aagtctattt tggttttaca ggttctacgg
     2341 gaggtgcggt caatacccac atcgtttgct tcaattacat agtttatccg gaagacccgg
     2401 agccgctgcc agatgctttc atctgcgagg gcgacagtgt gcagatggaa gcagcagaag
     2461 gcttcgttac ctatacctgg tcacccgcca ccggcgtgag cgatcccggt attgctgatc
     2521 cgtggttcag tccggtaacc accactacct attacctcac catgtcagat gaatgcggaa
     2581 tatctgaata tgacacggtg ctggtggaag tggaagaaga cgattctgaa ttcagttatc
     2641 cagccgatac ctattgtgct gaaggtactg tgctgccaga ttacatagaa agtcccggtg
     2701 ggtttttcag tattgaacca ccgggtcttc tgatcaacag tactaccggg cagctgaatc
     2761 tggatggagc cactcccgga acgtatgtgg tagagtacgt gccacctgca ggtatatgtc
     2821 cggtttcgag tactcagacc atcaccatcg agccattccc cgatgcgtca ttctcttatc
     2881 ctgaaacgat ctactgtgca gcaggcactg cattgcctgc agatgtcgtg gttggaggtg
     2941 gaagtttttc agcatcaccg gcagggcttt cagtcaatac ttcaaccgga ctcattgacc
     3001 tggccacagc aactatcggc gaaacatata ctatatatta tgacgtaaca gtgttctgtt
     3061 catcggccga cagttttacc atcaccatac aggattttga agatgcgggg tttgcgtatg
     3121 atgaaacaca ctattgcccg acaggtactg tgctcccggc attcatcaat actgcgggag
     3181 gcactttttc tgtttcacct gccggactgg tcgtggatcc atcgaccgga gaactggacc
     3241 tgagttcagg aaccgtcggc accaactaca ccattactta tctgacctcg gctacagact
     3301 gcggcgcttc gtcttctgtc accctgttca tagatccgct ggatgatgca tcctttgcct
     3361 attcagatga tcactactgt gctacaggta ccattctgcc ggattatatc accacctcag
     3421 gtggaagctt cactgcagat cctccctcac ttttggttga tgcagtaagc ggggaggtgg
     3481 acctggaaac aggcgcagta ggaacgacct ataccatcac ttacaccact gccgatggtc
     3541 cctgtcagaa ctgggatgct gtatccatca ccattgatgc caacgacgac tccacattca
     3601 gttataccga tgatacattt tgtccggaag gtgtggcact gcctgatctg atcgcaactc
     3661 ccggaggttc tttcactgcc tggcctgccg gacttggagt ggatgccttc agtggtctgc
     3721 tggatctcag taccgccaca ccgggaacct atacggtaac ttataccaca cctgtaagct
     3781 tgtgtacctc ttcgatggat ctgaccatca ccattgatac cgttacagat gcattttttg
     3841 catatgataa cccggcctac tgcagggtag gaagtatcat tccatttgtc ctgcatcccg
     3901 gaggtggttt cacggctgat gcaggtttgg tcattgacgc tgaaaccgga gaagtggatc
     3961 tggaatcctg tacacccggt ggcccgtatg cggtgtatta tcagagtcct ggatgcaccg
     4021 aagaagacac agtatggctg accatctggc ctgaacctga actgtccttt gcgcctgacg
     4081 gacctctgtg tattgaagac gcaccgcttc ttctcactgc tgatccaacc ggaggcagct
     4141 ttgcaggcac cggagtggcg ggaggtagtt ttgttccggg tgcagcagga gaaggtctgt
     4201 ttgagctaac ctattcctat actgatgtaa atggctgcag caatgagata tctgcatttc
     4261 tggaagtgat cgaaaattca gtcagtgtcg gagctgatgt aaccatcata gagggtacca
     4321 gtaccgcatt gcaggctgtg ggtggtgtca tcttcagctg ggagcctgca gatggactga
     4381 gttgtaccgg ttgtgcttca cccctggcat ctcctgcgca gaccaccacc tattgggtga
     4441 cctcctggga tgcatacggg tgtatggcca ttgataccgt aagggtagaa gtggttcctt
     4501 tcgatgatgt gacggtcttt gtacccaaca ctttcacgcc gaattttgat ggtatcaatg
     4561 attacctctt tgtgtatgga tctgatattg aacgcatcaa ctacctgcgc attttcgacc
     4621 gatggggaaa cgaggtctgg tttggtgaga acctgcctgc gggcatggag aataccggat
     4681 ggaatggtac ttccaacggc actccggtca acagtggtac ctatgccttc atcgcagagg
     4741 tacagctcac tttcggggta tcaaagcggg tcagtggaaa tgtgaccctg ttgcgttagt
     4801 caagccaaag acttaggcgg gtgtaactta tttttgttcg ataattccag ctatgaaata
     4861 ttgtctcatt ctgatctctg tgttgttggt tcaaggtgcc cgggcacaat gggtattaaa
     4921 tggtgatgcc gaagaactgg gcgataactg ttttcagctg acagaagaac tgcccaccca
     4981 ggccggatct gtctggtatg acagcctcat caacctggaa gaagattttg aggtcaactt
     5041 tgagatctat ctgggtacgc tggatgcttc cggtgccgat ggtattgcct tcgttctcca
     5101 gccggtcagt accggtctgg gcgaatctgg aggcggacta ggctatgaag gcattgagcc
     5161 atcgcttgat attgaattcg atacctggca gaacggtatt tacggcgatc ctacttatga
     5221 tcatatcagc atcatgcagg atggtatcct cgaccacttc gcggcaactg cactgactcc
     5281 ctacacccag atcctttcag gatcaactaa cgctgaagac ggaaactatc accaggtgcg
     5341 catcacctgg gaagcaggat cgcaaaccat cagttgtttt gttgactgtg atcttcgggt
     5401 cacctattcc ggcgatatca ttgatgatat attcgacggc gatccatttg tgttcatggg
     5461 ttttacagcc gccaccggtg ggtcctacaa tgaccagatc gtttgtctcg attatgttac
     5521 cgcagtagat gaactggatg atgttacagt ttgtccgggc gatagtgtgc aactattcct
     5581 tccggaaggt tttgtatcct attcctggtc gcccgcaacc ggtgtgtctg accctactgc
     5641 tcccgatccg tggttgtcgc cggatgtcac cacaacctac actgttacca tcaccgatga
     5701 atgcggcaat accatcacca acatagtgac ggttaccgta ggcagcccgg agctggtgga
     5761 tctgggccct gatctgagcc tctgtgaagg cgatgaggaa ctgatcagcg tattcctggc
     5821 aggagctacc tatgagtgga ataacggcag caccctgcct gcaatcacca ttacagagtc
     5881 aggcacttac tgggtagaac ttaccctggg taactgcacc gatgcagata ccatcaatgt
     5941 caattattcg ccggcaccat ttgtcaactt tggcccggat acagttgtct gcggactttc
     6001 ggaagtagta tttctggatg ccaccaatcc aggcgccacc tacctgtggc aagatggcag
     6061 caccagccca acctatacgg ttacagaaga cggtttgtat ttcgtgaccg taagcattgg
     6121 ttcctgtatc gatcaggatt cactggctgt aaactacagc tcctatcctg cggttgatct
     6181 ggggcctgac atatatctct gtcctgacag tgtagtgctt ttactggctg gcccgccggt
     6241 ctttgtctat acctggcagg acggcagttc aggaaacacc tatctgatca cagaatccgg
     6301 cgagtattgg gtggatgtga attccaacgg atgcatcaca cgtgcttata tagatgtgat
     6361 agacgaccca tgcatctgtg aaatgatcgt tcccaatgcc ttttcaccca ataatgatgg
     6421 ttacaatgat gaattcaggc aactggaatg ctcgcagctg aaccggttca atatgaagat
     6481 ctacaatcgc tggggtgagc tgatgttcga gaccagcgat tacagtgccg gatgggatgg
     6541 aactttccgg ggtgaacaat gtgagatggg agcttacgtc tttgtcattg aatacgacat
     6601 catagacggc gaaagcggca tcaaacaggg cagtttcctc ctgctgcgat aggggttcag
     6661 aaaaatatcc accaccatgt gaaaaaatac gacgggcccg gggggtccgt ttttttattt
     6721 tagccattcc gatacccgaa ttgcataatg aagttctcgc acctccataa ccacacccaa
     6781 ttctccctgc tggacggtgc ttcgcccatc aaaaagcttt atagcaaggc agtggaggat
     6841 ggtatgccgg cactggccat cactgaccac ggaaacatgt tcggcgtatt tcatttcgtg
     6901 gctgaagcag caaaacacac caacgaggat ggcagtctga aggtcaagcc catagttggc
     6961 tgtgagtttt atgtggtgga agatcggcac aaacgccagt tcacccgtga ggaaagagac
     7021 gtacgctatc atcaggtgct gctggcaaaa aatgatgtgg gctaccgaaa tcttgtcaag
     7081 ctgtgttctc tgggatatat ggaggggatg tacagcaagt acccccgaat agacaggtcg
     7141 ctgatcgaac aatatcacga aggcctcatt gccacgacct gttgtctggc tgctgaagtt
     7201 ccgagggcca tcatccgcaa aggagaagaa gaggccgaag aggtattcaa gtggtggctg
     7261 gatctttttg gcgatgacta ctacatagaa ctgcagcgtc acggtattcc ggagcaggac
     7321 gaggtgaatg ctgtgttgca gaaatggtcg gcaaaataca acgtgccgat gatcgcaacc
     7381 aacgactcac attatgtgga tcgtcaggat gcgaatgcgc atgacatttt attgtgtatc
     7441 aatactgctg agaaacaggc aactcccaag gcaaaggact tttcagatga agagggttcc
     7501 atgcggggta cccgttttgc tttctataat gatgaattct tcttcaagac ccaggccgaa
     7561 atgggcaagg ttttccggga tgttccggaa gcgctggaca ataccaatgc catcattgac
     7621 aaggtggagg tgctggacct gaagaaggat atcctgctgc cgcactttca gctgcctgcc
     7681 ggattcaccg atcaggatgc ctatctgcac cacctcacca tggaaggtgc gaaggctcgc
     7741 tataaggaca tcactcctga cattcaggaa cgattggact ttgaactgtt caccatcaga
     7801 acgatgggat ttgcaggcta cttccttatc gtggcagatt tcatcaacca tggcaaggac
     7861 atcggcgtat tcgtgggccc cggacgaggc tctgctgccg gctctgcggt ggcttattgc
     7921 attggcatca ccaacatcga cccgatacat tataatctcc tgttcgagcg cttcctcaat
     7981 cccgacagaa agagcatgcc cgatattgat accgacttcg acgatgaagg ccggcaaaag
     8041 gttatcgagt atgtggtgga caaatacggc aagaatcagg tagcgcagct catcaccttc
     8101 ggtaccatgg cagccaaaat gagtatcaag gacgtgggcc gagtactgga ccttccgctg
     8161 gcggaagcca acatgctggc aaagtatgtt ccggaacgcc cgggtatcaa cctgaacagg
     8221 ctcatgtttg caccgttaaa gggtgaagga agcctggcgg agaaagagaa tttgtcgccg
     8281 gatgaaatga ccaatatcca gcagctgaga aagatcatgc agggtgatga cctgcaggcg
     8341 aaggtcattc aggaagctgt caagctggaa ggctctgtgc gcaacaccgg tattcatgct
     8401 gccggcatca tcatcgctcc gtctgatctg acagatctgc ttccggtagc agtggccaaa
     8461 gattcagagc tgctggtcac ccagtatgaa ggcgagatca ttgaaagtgc aggcgtaatc
     8521 aagatggact ttcttgggct gaaaacatta agtatcctca agaccgctct caaacatatc
     8581 aaactcaacc accacagaga tattgatctg gatatactgg cactggaaga cgagccaact
     8641 ttcgggttgt atcaacgggg agagaccaat ggtacgtttc agttcgagag tgcaggtatg
     8701 cagaaatacc tgaaggaact taaacccgat aagttcgaag acctcattgc catgaacgcc
     8761 ctgtacagac cgggtcccat ggattacatt cccacctaca tagcaaggaa acagggcaga
     8821 gaaaagatca cctatgacct gcccgaaatg gaggagatcc tgaaagagac ctatggcgta
     8881 acggtctatc aggaacaggt catgctgctc agtcagagac tggccaactt caccaagggt
     8941 gatgctgata ccctccgcaa ggccatggga aagaaacaaa aggccgtgct cgacaagatg
     9001 aagagcaagt tcatggaagg ttgtgtggcc aacgggcatc ctgacaaggt ttgcgaaaag
     9061 atctggacag actgggaggc atttgcccag tatgcattca ataaatcaca ttccacctgt
     9121 tatgcgctgg tggcctatca gactgcctat ctcaaagcca attatccggc agagtatatg
     9181 tgtgccgtac tcacccataa ccagaataac ctggacaagg tgtctttctt catggatgaa
     9241 tgcaggcgca tggagatcat ggtactcgga ccggatatca atgagtcagg agtttcgttt
     9301 acggtgaatg aaaaaggtga gatccggttt gggctggccg cgatcaaagg agttggcgag
     9361 gcagccgtgc aggagatcat ccagcaacgt gaacaggacg gagcgtttca aacatttttt
     9421 gatctggtaa ggcgtgtcaa cctccgcacg gtcaataaga agaccatgga atcgctggtg
     9481 ttggcaggag cttttgatac attcggactg aaacgggcac agtattttac ccagcagaag
     9541 aatgatcagc tgaatgtact ggaacatgcc attaagtatg gtggcaccta tcagcaggga
     9601 agccagcagg aacaggctac attgtttggc gatgcagtgg aggtggagat cgcagaacca
     9661 acagttcctg atgtaccgga gtggaacagt gtagataagc tcaagcagga atatgatgtg
     9721 tgcggcatct acctgagtgg tcacccgctt gatgaacacc gtctggaagt ggaagctttc
     9781 cgcacctgtt ccattgcaga tatgcatcag ttcgtgggaa gaaccatcag cctgatcggt
     9841 ctggtgggca gttcacaaac caggaccacc aaaaccggag ataagttttc catcttcacc
     9901 ctggaagact attccggtaa tctggaatgt atgctcttca aggaaatgta tgtgaagttc
     9961 aaacactacg tagaagaccc gggctacatc atccatgtaa aagggaattg tcaacccggc
    10021 tatcgtgata aggagaagat agaggttcgt gtgcaggaag tggaactgtt gagtgagaca
    10081 agggccaaac gttccatcaa agtgcgtctt aagatgcctt tggcaagggt agataccaaa
    10141 cagatcgttg atctgaaggc cattctggat gcgcatcccg gaaacgacca gctggccatg
    10201 gaggtctatc atgaacagat gcaggtgccg ctccgttctg tcaatggcct ggtgcaggta
    10261 gataaagggc tgatcaaagc gctgggaagg tttggcgaag tgcagctggc ggttgggtga
    10321 ggttgcgtat ctttgccggg aacttatctg cccgtcagct ggtttgcaga atacaagaat
    10381 aatcaagaac aactatagca aatggcaaca gaatttacag acgctaattt caaggagcag
    10441 gtccttgata gcgacaaact gaccgtgatc gatttttggg ctgtgtggtg cggcccctgt
    10501 cgtatggtag ctcccgtgat cgaagagctg gccaaagatt atgatggaca ggtcaacatc
    10561 ggaaaggtag atgtggacag caatcctatg gtcagccagc aattcggtat ccggaatata
    10621 cctaccatcc tcttcgtaaa aaatggagaa gtggttgaca agcaggtagg agctgttcct
    10681 cgctcagtgc tggaagccaa gatgaagcag cacatgtaag tgtgtgccgg taacatacca
    10741 gttaaagcct tctttcgttt aaagaaggct ttttaatttc acccaatgca aacggaacta
    10801 ttacgtcttc aggatatccg ccaggtagag cacctggaac tcctggccaa acaggtagtg
    10861 gaaggcttta ttctgggttt gcataagagc ccctttcatg gtttctctgt cgaatttgct
    10921 gaacaccgga tatacaatcc cggtgaaagt acccgctata ttgactggaa ggtttttggc
    10981 cggacagatc gtttgtattc caagaagttt gaagaagaga ccaacctgcg ttgccagctg
    11041 gtcatagata cttcctcgtc catgtacttc ccggaacaac cggcgccttc cgggaaattc
    11101 accatgaata aggtggtctt ttctgctgta tgtgccgcag ccatcatcca gatgctccgc
    11161 ggtcagcggg atgcagccgg cctcaccctg ttcagcgatg ctgtgcacct gcataccaaa
    11221 gccggatcag ggcatgttca ccaccagctg ttgttcagta atctggaaaa attcctgggc
    11281 gaaaaaagca gaaataacaa gacccgggca gcagattgtc tgcatcgcat cgcagaacag
    11341 atacaccgca gaagcatggt ggtcatcttc tcagatatga tggaaggcgg tgaagatcag
    11401 gatgacctct tttctgcatt gcaacacctt aaatacaata agcatgaggt agtgcttttt
    11461 catgtggttg atcacctgaa ggaactggaa tttgaatatg agaacaggcc atatgtattt
    11521 gtggacatgg agacaggcga aaaagtgcgc atccagggta atgagatcag aacgcattac
    11581 aaagaacgca tgcagaaata tctgaacaat ctgaaactgc gttgccatca gtatcagatc
    11641 gattttgtgg aggcagacat caacaaagat ttcaaacagg tgctaatgcc ctatctggtc
    11701 aagcggatga aaatgaaata aagcatggca gttctgcatt tcaaatcttc aggtaaagga
    11761 gaacctctca tcattctgca cggtttgttc ggtatgctgg ataactggca gaccatagcc
    11821 aaccgcctgg acaacagata cctggtctat ctggtggacc tgcgaaacca cggaaggtcg
    11881 ccgcacatgg agtccatgac ctatcctgaa atggctgaag atatagttca gctgatggat
    11941 gcagaaggca ttccggctgc taatattctc ggacattcca tgggtggcaa agtagccatg
    12001 cagctggcat tcgatcatcc tttacgggta cagaaactta tcgttgcaga tatcggtccg
    12061 aaaggttacc ctcctggcca tgaccagatc ttcgaggcgc tggatgcggt ggttcttccg
    12121 gccgtcacac aacgaagaga tgcagaagag atgatgcaac aggtattgca cgacccgggt
    12181 atcatccttt ttctgatgaa gaatctgcaa cgccacaccg atggctctta cggttggcgc
    12241 atgaacctgg aggtgatcag agaacagtac tcgtccattc gcggaccggt atcaggagaa
    12301 tcatttcatg accccgctct ttttatacgc ggcagcagat ctgattacat ccctgataat
    12361 gactggcctg ccattcagga gctgttcccg ctggcagcac tggtcactat tcccgatgcc
    12421 ggccactggg tgcatgctga tcagccagac atgctcctga atgaggtggt caggttcctc
    12481 ctatcatgag tatctttgtt taaacaaagt cataactatg aaagcaattg tactgtctgt
    12541 tgcagtattg gtatgcgctg tggggtacac ccagccggtt attactgcag atgtcttacc
    12601 ggaggtggct gagagcttca gcctgacacc ctttgatgcc acaggttttg atccgggtga
    12661 tactggtgca ggtgttacct ggaatttcag cgatcttgac gcaagcggat cggtggaaac
    12721 aactgtggct catgatgcct ccaccattgg tggtcacgag gattttcctg aagccaatgt
    12781 tgcgtggaca aacgacggac tgacgtttac tttcttcaag tcaaacagca gtgtacttga
    12841 aaactgggga agtgttgtca atgatgctga tctgggtttt atttctatcc catacagtga
    12901 tggggaggaa ctcctgacct taccactgaa ttttggagat acgaacgaag atgattttga
    12961 cagagcattc tcagcaggag gatttgatgt ggca
//
LOCUS       DAOMAX010000055        12482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_640_length_12482_cov_4.375392, whole genome shotgun sequence.
ACCESSION   DAOMAX010000055 DAOMAX010000000
VERSION     DAOMAX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12482
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_12482_cov_4.375392"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..961)
                     /locus_tag="PK742_04620"
     CDS             complement(368..961)
                     /locus_tag="PK742_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HQU75907.1"
                     /translation="MRLTLLFTFLLMVCSARAQFYSDVVADTIFTFKDKRDNNKYTAV
                     KIGDMIWMGENLRYKTKDSWCYKDKELNCEKFGRLYEWEEAQTACPPGWHLPSMAEFD
                     SLVARLGGDKKAGYALAYDSGVGMQMKFGYPPNVNGRFNAEDSQTNFWTSSENNKDTA
                     WSYYMIKIKLPLVFSSYFSKNYNMSCRCVKDDSEIGG"
     gene            1049..1723
                     /gene="mqnB"
                     /locus_tag="PK742_04625"
     CDS             1049..1723
                     /gene="mqnB"
                     /locus_tag="PK742_04625"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HQU75908.1"
                     /translation="MNVLLVSATHLEIMPLLQRCDWPSGWGPVYSTMIGKHTVDVLLS
                     GPGVVAASYQLGTLLGMRRYDLAVQAGIAGSFRQELAPGKVVHIKQDILADFGSSSPD
                     GFLPAASIGLLESSAYDTSEGHLLETAGISWPESVTAANGITVQQVSGTEADIASMRQ
                     HFDPDTESMEGAAFYFACRMHRLPAAQLRSISNLVEPRNKENWNIPLAVAQLDVALQL
                     LFEELL"
     gene            1720..2553
                     /locus_tag="PK742_04630"
     CDS             1720..2553
                     /locus_tag="PK742_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HQU75909.1"
                     /translation="MKLTLGFSPCPNDTFIFDALVNGHLNTGDLEFEVVLEDVETLNK
                     MAKEGKLDITKLSFHAYAYVSDQYQILQAGAALGKGCGPLLISADPIPLSKVDYCVVG
                     IPGRLTTANLLFSLAFPDAITKKELVFSAIEQELLDDRIDVGVIIHENRFTYESKGLH
                     KLLDLGTFWEEKYGVAVPLGGIMIRRSLGDAVQHRVNDLIRQSVSYAFSHPEVSAPYV
                     AQHAQEMDPAVMQQHISLYVNEHSLDLGDDGRSAIEHMYRETHAIGLTPAVLQPIFAG
                     R"
     gene            complement(2527..3132)
                     /locus_tag="PK742_04635"
     CDS             complement(2527..3132)
                     /locus_tag="PK742_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQU75910.1"
                     /translation="MKDEGVIKYSQVWKRNVLPSLKEADALLAIRDALFRRHWIGTDA
                     DGVGFGNVSVRLPDNAFLITASQTGHIPHLSTVQLSMVTAVDINENTLWCTGLMPASS
                     EAMSHAAIYAVKPDVHCVIHVHDAELWNSLIHVAPTTDAGIPYGTPDMAYALQELILD
                     MEDNELIVMGGHPDGIISAGSNTDDAWQTLQNWSAAGKDRL"
     gene            complement(3134..4225)
                     /gene="mtnA"
                     /locus_tag="PK742_04640"
     CDS             complement(3134..4225)
                     /gene="mtnA"
                     /locus_tag="PK742_04640"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQU75911.1"
                     /translation="MNVQGTPYRTIWPDLSDPHRIHIIDQRQLPHKLEIVSLKKVEDA
                     ALAIRDMWVRGAPLIGATAAWGMYLAAADIPARCNMQFYFEEVYHLLLSTRPTAVNLK
                     WALDRMLTVLRDAGDMDEARLLASRMARQIAEEDVWANERIGDHGVELIREIYQNKQR
                     PVNILTHCNAGWLATVDWGTATAPLYKAHLQGIPVHVWVDETRPRNQGASLTAWELQQ
                     MGVPHTLIVDNAGGHLMQQGKVDIVITGADRVDAEGYAANKIGTYLKALAAADNEIPF
                     YIAMPSSTIDWETRGGQIPVEERSQDEVLNIQGKLGNKIVSVNLAPEHTIAANPSFDI
                     TPPRLITGYITERGRSDIGWKGITALFSA"
     gene            4281..5207
                     /locus_tag="PK742_04645"
     CDS             4281..5207
                     /locus_tag="PK742_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75912.1"
                     /translation="MKNVLLVSFLFLWQPIFGQSVFVLDQEEKLLGRISGDSVYTGPE
                     EVTFVLRGQLIRSLRDNRSWLVDCDDFFGRKAGLVKTNGGKTISCIIRKGSVFLGDHP
                     VDENHEKLLQLVRQDSVHYLVLHGLSGDTLGHVTGAPDDAGMLFAISLLYMETFQLEQ
                     DIAEHLRWMEEQRNVPAEARIYPLMDSSPTREWTWDGAQFRFYLGGRLQSVWVYDGRR
                     LRCTEGLAAGMEWTWESGVLRPSFDPDPNKQWTWTGEQLQPYWGSNPDQMWTLNGNIL
                     RPTWNADTRLQWVVEGEFPLPALALIVLGYAR"
     gene            5255..5758
                     /locus_tag="PK742_04650"
     CDS             5255..5758
                     /locus_tag="PK742_04650"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQU75913.1"
                     /translation="MKKILFAALLGVVTLGAVQAQTVKIGYINSMELLSMMPEAKEAD
                     AKLQKMAKDLEAQYREYVLEYQRQLSILQSDSTLSEVAREAKIQDLTNLEQRITDFES
                     SSQEKLNTKRSELYEPILDGANKIVQEVAKDNGYTHVFDSSTGAIIYAPEGDDIIELV
                     KTRMGIQ"
     gene            6324..7853
                     /locus_tag="PK742_04655"
     CDS             6324..7853
                     /locus_tag="PK742_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75914.1"
                     /translation="MKNLIEISKIVTKKRISKIEIFDKSLLNKKDSKFNEFYEGLVNN
                     QFRNDDEAAQFLYDTTPLDDKYRQLKSRFTKRLMNTLFFLDANDPSFSNYHSAYYTCN
                     KNWALIRILLSSGARNAATKLANKTLHVAQGFRFSDIILNCSRILMSNSSLTGRLREY
                     DEFSLIANKAMKDLQAEMESEEYYQRLTIGLATSASIDEERAQLAEDYLVKVSRLCDE
                     SDSYILHFNMHQIRILALQFRGNYTGTIKACEEAEQFLEENPPFRQEVKLAALALIRI
                     NCYLQLRDYENGRKNAEKCLELFSEGTNNWFVFLEQYFLLAMHTENYINAAGIYLKVI
                     NHPRFPFTASERQEKWKIFEAYINYIFETENLDLDLLTDEGKRKFRILKFLNEVPHFS
                     KDKKGFNVAILVIQILYMLEKGDYTGIINRSEALNVYCSRYLRKDDSYRSSCFLKMLL
                     TMEKEDFKYDRTKQMAMKYLKKMTEDETGDNTVNEWEIIPYETLWEKVLNRLQNKVAA
                     A"
     gene            7850..8245
                     /locus_tag="PK742_04660"
     CDS             7850..8245
                     /locus_tag="PK742_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQU75915.1"
                     /translation="MKHLILIPCILFSMLANAQLRQPMEEITEPADLETIKVQKLYED
                     SLNTTYVIWVRTEVPKHYHAEHTETIYVLEGIGLMTLGDQVFNIVSGDLLVVPFGTPH
                     SVKTMSKDPLKVISVQSPFFDGSDRIPVK"
     gene            complement(8251..9168)
                     /locus_tag="PK742_04665"
     CDS             complement(8251..9168)
                     /locus_tag="PK742_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU75916.1"
                     /translation="MEEKKQVRILLVEDNPADQRLVEIYLQESTMLDSAVTKVGELRK
                     GLLKLEEGDFDVVLLDLTLPDSSGFTTIQTMLEEFPEQTVIVMTGLEDEVVALNSVKA
                     GAQDFIVKGQFDSNLLSRTITYAIERHQLQMKLENYARAIKRNEERLLQAQRMARIGN
                     WELDIVTNQMQWSDEVYRIMGIKEGSIEPTLQDYLKYVLPEDAASVKSAINRTMEKGQ
                     PYQVEYMMKLPNGTIKHIANAGQIQVNRKSGGLSLSGTIQDISAFRSNNNGTDGAQMK
                     ETLKNALKMLEDGADADARTTIRTLMEKL"
     gene            complement(9172..9627)
                     /locus_tag="PK742_04670"
     CDS             complement(9172..9627)
                     /locus_tag="PK742_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU75917.1"
                     /translation="MAQQPEVMNILLVEDNPGDIRLTQEAFKEGTIPKTLHVVKDGVE
                     AMEFLRQRGKYAEAPMPDIILLDLNLPRKDGREVLADIKADEHLRFIPVIILTTSDSE
                     QDISRAYSLYANCFVTKPVDLEQFIFIIRQIEIFWFQVTKLPTRHKYQN"
     gene            complement(9719..10369)
                     /locus_tag="PK742_04675"
     CDS             complement(9719..10369)
                     /locus_tag="PK742_04675"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HQU75918.1"
                     /translation="MRGLFIAGLLCISVAASAQSYSIALLKYNGGGDWYANPTSLGNL
                     VAYADKYAGMDIDSEPATVEVGSRDLFNYPFAHMTGHGNVVFSDAEAENLRKWLMAGG
                     FLHIDDNYGMDPYIRPALKKVFPDQELTELPFSHPIFHQLYNFNGGLPKIHEHDGKPP
                     QAFGLFYEGRLVCLYTFECDLGDGWEDPDVHNDPEPVRQKALQMGVNILQFVFTAI"
     gene            complement(10366..11082)
                     /locus_tag="PK742_04680"
     CDS             complement(10366..11082)
                     /locus_tag="PK742_04680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQU75919.1"
                     /translation="MDSKAPRFILDDLLESDVLDLPDQEGRHAMQVLRMQFGDTVELT
                     DGKGNLAIATITHAGKKHCSVAVHERLSEQPIEPAVTLAIAPVKSMDRMGWLLEKLTE
                     VGISRIIPVITDRSERRSIRSDKAIATMQSALKQSRRCWLPEWQEPVSFADFWKQQNE
                     NVFFAHCMAGEKQLLSRCVPPGQPCTILIGPEGDFTPEELQLALENKAIPVSLGEQRL
                     RTETAALQAVITVHLLNGKL"
     gene            complement(11101..11907)
                     /locus_tag="PK742_04685"
     CDS             complement(11101..11907)
                     /locus_tag="PK742_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQU75920.1"
                     /translation="MKILRVKFSAALFTILFSAVILSFGNNKGRKYLEGLGFHYIPAG
                     VVYSDSGEVSVRGFYMYEAEITNGQYAEFLQYLKKHDRQADLQMAGIHNGNWETALSF
                     ENTYAADYHTDPAFVDYPVVNITYEAAQLFCDYLQEMIREKVPDAYVRLPDEIEWIHA
                     AKGGKNDAEYPWENLGVNGKKEVEFCNYNSPDDGTCLATNLSRSYYPNAYGLYNMSGN
                     VAEMLETKGRTKGGSWGSTAIQMRITAPDEYAGFDDASPFIGFRPVVILP"
     gene            complement(12052..>12482)
                     /locus_tag="PK742_04690"
     CDS             complement(12052..>12482)
                     /locus_tag="PK742_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75921.1"
                     /translation="GPGGNIIVLPSNGTPPYTIVWNDAFLSGDTIIGTWPGTYILTVT
                     DANGCSVVDTVMVPIGTYLSNEAQSILVYPNPFNDMLSIYTDIREGHLSVYDMNGSAV
                     LSKTQFSENITLSTALWPAGMYMMKLEGSDFFRMFLIVRN"
BASE COUNT         3192 a         3037 c         3011 g         3242 t
ORIGIN      
        1 aaaaagcctt ccgcaggaag gatcatttgg aagtttgcgc ccccacttac cggatggccg
       61 aaggcaatct gcttccggtt catgatgtat gaatcatttt gcccttactg caggctgagc
      121 ttgctcaaag ccgaaaggaa caggcaaaaa aaaagccttc cgcaggaagg atcatttgga
      181 agtttgcgcc cccacttacc ggatccactt gcaacagaga aataatacgt tacctacgag
      241 cagagcgcac tcagaactga atggaatctg agaaaaaaga aggcattatt taaaaaaact
      301 cactgctcac atttcacttc tcacacctgc ctctttccgc atatgactgt aaagttcaga
      361 acaagatcta tcctccaatt tcactgtcat ccttcacaca gcggcaactc atattatagt
      421 tcttggaaaa atagctgctg aacaccaatg gcagtttgat cttgatcata tagtaagacc
      481 aggctgtatc cttattgttt tcagaagagg tccagaaatt ggtctgcgaa tcttctgcgt
      541 tgaagcgccc gttcacattg ggcgggtagc cgaatttcat ctgcatcccc acaccactgt
      601 cataggcaag agcgtatccg gcttttttat cgccacccag acgggcaacc aggctgtcaa
      661 actcagccat agacggcagg tgccatcccg gtggacaagc ggtctgcgct tcttcccatt
      721 catagagtct gccaaacttc tcacagttca actctttgtc tttataacac caactgtctt
      781 tggtcttata ccgaaggttc tcacccatcc agatcatatc tccgatcttg actgcagtat
      841 atttgttgtt gtctctttta tctttaaatg taaagatggt atctgccaca acatcagaat
      901 aaaactgagc ccgggccgaa cataccatca gcaggaaagt gaacaataat gtgagtctca
      961 tgcctggagt ttgattggag gtaagtgcaa atttcgtgct atttttccgc aacataaagc
     1021 tacaggagtt tcatccaacc tgcttatcat gaatgtacta cttgtaagcg ctactcacct
     1081 ggagatcatg ccgttgctgc agcggtgtga ttggccatca ggctgggggc ctgtgtattc
     1141 caccatgatt ggtaagcata cagtggatgt gctgttgagc ggcccgggtg tggtggctgc
     1201 atcctatcag cttggaaccc tgctgggcat gcgaaggtat gatctggctg tgcaggccgg
     1261 aatagccgga agtttcaggc aagaacttgc acccggaaag gtggtacata ttaagcagga
     1321 tatacttgca gactttggca gttcatctcc tgatgggttt ttacctgcag catctatagg
     1381 cttgctggaa tcatctgcat acgataccag cgaaggacat ctgctggaaa cagccggtat
     1441 aagctggccg gaatctgtca ctgccgccaa tggtattaca gtgcaacagg tcagtggaac
     1501 tgaagcagat atagccagca tgcggcaaca ctttgatcct gacacggaaa gcatggaagg
     1561 cgctgcattt tattttgcgt gccgcatgca caggttgcct gctgcgcagt tgcgttcgat
     1621 ctccaatttg gtagaacctc gcaataaaga gaattggaat ataccgctgg ctgttgcgca
     1681 actggatgta gctttgcagc tgctatttga agaactgtta tgaaactgac actgggtttt
     1741 tctccctgcc cgaatgatac attcattttt gatgcgctgg tgaacggaca tctgaatacc
     1801 ggcgatctcg aatttgaggt tgtactggaa gatgtggaaa ctctgaataa aatggccaaa
     1861 gaaggcaagc tggacatcac caagctttcc tttcatgcct atgcttatgt gagtgatcag
     1921 taccagatcc tgcaggccgg agctgctttg ggaaaaggtt gcggcccgct tcttatcagt
     1981 gcagatccga taccactttc caaagtggac tattgtgtgg tgggcattcc cggcagactt
     2041 accactgcca acctgttgtt cagcctggct tttccggatg ccattaccaa aaaggaactg
     2101 gtcttttctg ccattgaaca ggagttgctg gacgacagga tcgacgtggg agtcatcatt
     2161 catgaaaacc ggttcaccta tgaatcaaag ggattgcata aactcttgga cctgggaaca
     2221 ttctgggaag aaaaatacgg tgtggcagtt ccgcttggcg gaatcatgat acgccggtca
     2281 ctgggagatg cagttcaaca ccgggtcaat gatctgatca ggcagagtgt ttcctatgct
     2341 ttcagtcacc ctgaagtatc agcaccctat gttgcacagc atgctcagga aatggatcct
     2401 gcggtgatgc agcagcacat cagcctgtat gtaaatgagc acagtctgga tctgggtgat
     2461 gacggaagaa gtgctataga acacatgtac cgtgaaacac atgccatcgg gttgaccccg
     2521 gctgtcttac aaccgatctt tgccggccgc tgaccagttc tgcagggtct gccatgcatc
     2581 atcggtgttg ctgccggcac taatgattcc atcaggatga ccgcccatga cgatcaattc
     2641 gttgtcttcc atatccagga tcaattcctg taaggcataa gccatatcgg gtgtgccata
     2701 aggaataccg gcatcggttg taggagccac atgtatcaaa ctgttccaga gttctgcatc
     2761 atgcacatgg atcacacaat gcacatccgg ctttacagca taaatggcag catggctcat
     2821 ggcctctgaa gaagccggca tcagtccggt gcaccacaat gtattctcat tgatatctac
     2881 cgcagtgacc atagacagtt gtaccgtgct gaggtgtggt atatgaccgg tctggctggc
     2941 agtgatcaga aaagcattgt caggcaggcg aacagacaca tttccaaatc ccacaccatc
     3001 ggcatctgta ccgatccagt gcctgcgaaa aagcgcatcg cgaatggcca gcaatgcatc
     3061 cgcttctttc agggatggaa gcacatttcg tttccacacc tgactgtatt taatgacacc
     3121 ttcgtctttc atgtcatgca gaaaaaagtg ctgttatacc cttccacccg atatcagatc
     3181 ttcccctttc ggtaatatat ccggtgatca ggcggggcgg ggtaatatca aacgacggat
     3241 tagctgcaat ggtgtgttcc ggtgccaggt ttacagatac gatcttgttt cccagtttcc
     3301 cctgaatgtt cagcacttca tcctggcttc gttcttccac cggtatttgt ccgccccggg
     3361 tttcccagtc tatggtagaa gatggcatgg caatataaaa tggtatctca ttatctgctg
     3421 cagccagggc tttcaggtag gttcctattt tattggcagc atatccttca gcatccaccc
     3481 ggtctgcacc tgtgatcact atgtccacct tcccctgttg catcagatgt ccgccggcat
     3541 tatccacgat cagcgtatga ggcacaccca tctgttgcag ttcccatgcg gtcaatgatg
     3601 caccctgatt tcttggcctg gtttcatcca cccaaacatg aaccggaata ccttgcagat
     3661 gcgctttata taaaggagcg gtggcagtgc cccagtcaac cgtagccagc catcccgcgt
     3721 tacaatgggt aaggatattg acaggccgct gtttgttttg atagatctct ctgatcagtt
     3781 ccacaccgtg atctccaatg cgttcgttcg cccacacgtc ctcttcagcg atctgtctgg
     3841 ccatgcgtga agcaagcagt ctggcttcat ccatatcgcc cgcatcacgc aagactgtca
     3901 gcattctgtc cagggcccac ttcaggttca cggcggtagg ccgggttgac agcagcagat
     3961 gatatacttc ttcaaaatag aactgcatat tacatctggc aggaatatcg gcagcagcca
     4021 gatacatgcc ccatgcggct gtagctccaa tgagaggtgc tcccctcacc cacatatcgc
     4081 gaatggcgag ggccgcatct tccacttttt tgagggaaac gatctccagt ttgtgaggca
     4141 attgccgctg atcaatgata tggatccggt gtggatcaga cagatcaggc cagatggtac
     4201 gatatggtgt gccctgaacg ttcatgttat accggcaaag atggcaaacg ccagccagat
     4261 tcaaccctta cctttatttc atgaagaatg tgctgttggt ttctttcctg tttctatggc
     4321 aaccaatctt tggccagtct gtttttgtac ttgatcagga ggagaaactg ctgggaagaa
     4381 tttcaggtga cagtgtgtac accgggcctg aggaagtgac ttttgtgctg agagggcagt
     4441 tgatcaggtc tttgcgggat aacaggtcat ggttggtgga ctgcgatgat ttttttggca
     4501 ggaaggccgg actggtaaag accaacggag gaaagaccat atcctgtatc atacggaagg
     4561 gaagcgtttt tctgggagat catccggtag atgaaaacca tgaaaagctg ctgcagctgg
     4621 tcaggcagga ttcggttcat tacctggtgt tacacggcct ttccggagac acgctggggc
     4681 atgttacagg agcgcctgat gatgctggta tgctttttgc catttctctg ctgtatatgg
     4741 aaacctttca actggaacag gatattgccg aacacctgcg gtggatggaa gaacagcgca
     4801 atgtgccggc agaagcacgt atctatccgc tgatggacag cagtccgacc cgcgaatgga
     4861 catgggacgg agcgcaattc cggttttatc tggggggtcg tttgcaatct gtgtgggtat
     4921 atgacggacg aaggctcagg tgcacggaag gacttgctgc cggtatggag tggacatggg
     4981 agtcgggcgt actaaggccg tctttcgatc cagatccgaa taaacaatgg acctggacag
     5041 gcgaacagtt gcagccatac tggggcagca atccggatca gatgtggacg ctgaatggaa
     5101 atattctgcg gcccacgtgg aatgctgaca caagattaca atgggtcgtg gagggtgaat
     5161 tccctttacc cgcactggct cttatagttt taggctacgc acgataagtg aaaacggtat
     5221 tattatattt gcacctcaat ttgaagcgat caacatgaaa aaaatcctct ttgctgccct
     5281 tttgggcgta gttacccttg gcgctgtgca ggcacagacc gttaagatcg gctacattaa
     5341 ttcaatggag ctgctcagca tgatgccgga agccaaggaa gcagatgcca aactgcaaaa
     5401 aatggccaaa gacctggaag ctcaatatcg tgagtatgtg ctggaatacc agcgtcagct
     5461 tagtattctg cagtcagaca gcaccctgag tgaagtagcc cgggaagcca agatccagga
     5521 ccttacaaac ctcgagcagc gcatcactga ttttgagtcg agctctcagg agaagctcaa
     5581 taccaagcgt tcagagctgt atgaaccgat attggacggt gccaacaaga tcgttcagga
     5641 ggttgccaaa gacaatggtt acacccatgt ttttgacagc agtacaggag ccatcatata
     5701 tgccccggaa ggagatgata tcatagagtt ggtgaagacc cggatgggta ttcagtgatc
     5761 cttatctgat aagactgcaa agccgttcca acaggagcgg cttttttttg tgcaacaaaa
     5821 aaggccctga cgggcctttc ttgttcgtga acctaattta tgaaacaaac caactgtact
     5881 ttacagttca tccgggtcaa tgatcactat catcgggatg atctctggag tattatttct
     5941 tttcatagcg caatggattt atcgggttag caatctaaga acaatgctaa ggtataagca
     6001 gaatcggata gttggcatac agtagagacc aatttgtaac atttttaatt gcaagattat
     6061 tgttcctatt gctcaatagt aaggctttta tctaaatatt taaagatttt ttgtgacttt
     6121 tttactgctt tttgtgcatt ttccgggaat aaccgccaga accctagtct tacaataagt
     6181 ctgcaagata taccgctgat ttattatttg caaaattgat ttgtaggatt atatttgtga
     6241 caagtaatga tgctgatatc tatcaaaatg cagtgtccaa ttcatataat aaactgtcaa
     6301 tcaattactt aacctgatca cacatgaaga acttaataga gatcagcaag attgtgacca
     6361 aaaagaggat ctccaagatc gagatctttg acaagagcct gctgaacaag aaagacagta
     6421 agttcaatga attctatgaa ggtctggtca acaaccagtt caggaatgat gatgaagccg
     6481 ctcagttcct gtatgacacc accccgctgg atgacaagta ccgccagctg aaatccaggt
     6541 tcaccaagcg tctgatgaac accctgttct ttctggatgc caatgaccct tccttttcca
     6601 actaccacag tgcctattac acctgcaata agaactgggc tcttatccgt attctcctga
     6661 gttccggagc cagaaatgct gccaccaagc tggccaacaa gacccttcat gtggcacagg
     6721 gattccggtt ctcagatatc atcctgaatt gttcccgcat cctgatgagc aacagcagcc
     6781 tgacaggcag gttgcgggaa tatgatgagt tcagcctgat cgccaacaag gccatgaaag
     6841 atcttcaggc cgaaatggag tctgaagagt attaccagcg cctcaccata gggctggcca
     6901 caagcgcctc cattgatgag gaaagggcac aactggccga agactatctg gttaaagtga
     6961 gccggctctg tgatgaaagt gactcctata tcctccattt caacatgcac cagatcagga
     7021 tcctggcgct gcaattcagg ggtaactata ccggcaccat caaggcatgt gaggaagctg
     7081 aacagtttct ggaagaaaat ccgcctttcc gccaggaagt gaagcttgcc gcactggcac
     7141 tgatacgcat caattgttac ctgcagttgc gtgactatga aaatggcagg aagaacgcag
     7201 aaaaatgtct ggaactgttc agcgaaggca ccaataactg gtttgttttt ctggagcagt
     7261 attttctgct ggccatgcat acagagaact acatcaacgc tgcaggtatc tatctgaaag
     7321 tgatcaatca tccccgcttt ccgttcacag catcagaaag gcaggaaaag tggaagatat
     7381 ttgaagctta tatcaactac atttttgaga ccgaaaacct cgacctggat cttctgaccg
     7441 atgaaggcaa gcgcaaattc cgcatcctga aattcctcaa tgaagtgccc cacttctcca
     7501 aagacaagaa aggattcaat gtggccattc tggtcattca gatcctgtat atgctggaaa
     7561 aaggcgacta taccggcatc attaaccggt cagaagcact gaatgtctat tgcagccgct
     7621 atcttcgtaa agatgacagt taccgaagtt cctgcttttt aaagatgttg ctcactatgg
     7681 aaaaggaaga cttcaagtat gaccggacca aacaaatggc catgaaatac ctgaagaaga
     7741 tgacggagga tgaaacaggc gataatactg tgaatgagtg ggagatcatt ccatacgaaa
     7801 cactgtggga aaaggtactg aatcgcctgc aaaacaaagt agcagctgca tgaaacacct
     7861 cattctgatt ccctgtatcc tgttcagtat gctggcaaat gcacaattgc gccagcccat
     7921 ggaagagatc actgaacccg cagatcttga gaccatcaag gtacagaaac tctatgagga
     7981 cagcctgaac accacatatg tcatttgggt gcgcacggaa gtgccgaagc actatcatgc
     8041 tgagcataca gaaaccatct atgtgctgga aggcatcggt ttgatgaccc ttggcgacca
     8101 ggtgttcaac attgtttccg gcgatctgct ggtcgtgcca ttcggcacac cgcacagcgt
     8161 aaagaccatg agcaaagacc cgcttaaggt cataagcgtg cagtcgccat tttttgacgg
     8221 atcagaccgc attcctgtca agtaaaaaga tcacaatttc tccatcaggg ttctgatagt
     8281 agtccgggca tctgcatctg cgccgtcttc cagcattttt aaggcgtttt tcaatgtctc
     8341 tttcatttga gcgccatccg ttccattatt gttgctgcga aaagccgaaa tatcctgtat
     8401 ggttcctgac aaagacaatc cacctgattt cctgttcacc tggatctggc ctgcattggc
     8461 aatgtgcttg atcgtgccat tgggaagttt catcatgtat tccacctggt agggctgtcc
     8521 tttttccatg gtgcggttaa tggccgactt caccgatgct gcatcttcgg gcagtacata
     8581 cttcagatag tcctgcaggg taggttcaat actcccttct ttgattccca tgatcctgta
     8641 cacctcatca ctccactgca tctggttggt aacgatgtcc agctcccagt ttcctatccg
     8701 ggccattctt tgtgcctgca gcagtctttc ttcattccgt ttaatagccc gggcatagtt
     8761 ctcgagcttc atctgcaact gatgacgttc aatggcgtag gtgatggtgc ggcttaacaa
     8821 attggagtcg aactgtcctt tgacgatgaa atcctgggca cctgccttca ctgagttcag
     8881 ggcaactacc tcatcttcca gtccggtcat gactatgaca gtctgctccg ggaactcttc
     8941 cagcatggtc tgaatggtag tgaaacctga actgtcaggc agggtaagat ccaacagcac
     9001 cacatcaaaa tctccttctt ccagtttcag cagacctttc cggagctcac ccaccttcgt
     9061 gaccgcagaa tccagcatgg tggactcctg cagatagatc tctaccaaac gctggtctgc
     9121 aggattatct tccaccagca gtatgcgcac ttgcttcttc tcctccatgg gtcagttttg
     9181 gtatttatgc ctggtaggca atttggttac ctggaaccaa aatatctcaa tttgccgaat
     9241 gataaaaata aactgctcca gatctaccgg tttggtcacg aagcagttag catacaggct
     9301 ataggctctg gagatatcct gttcactatc ggaggttgtc aggataatga ccggaatgaa
     9361 gcgcaggtgt tcatcggcct tgatatcggc cagtacttcc ctgccgtctt ttcgcggcag
     9421 gttgagatca agcaggatga tgtcgggcat gggagcctca gcgtactttc cccgctgcct
     9481 tagaaattcc atggcctcca ctccatcttt tacgacatgc agtgtctttg gtatcgttcc
     9541 ttccttgaac gcctcctggg ttagccggat atcgccggga ttatcctcta ccaggagaat
     9601 gttcatcact tcgggttgtt gtgccatcac ggattttgga caaaatataa aataattgtt
     9661 acacccgggt aactgaccgg gctacaggag gagagatgtc aaagttaagc gatagccttc
     9721 agatggccgt gaacacgaat tgcaaaatgt tgacacccat ttgcagtgcc ttttgcctga
     9781 ctggttcagg gtcattatgc acatccgggt cttcccagcc atcacccagg tcacattcaa
     9841 aggtgtacag gcagaccaga cgaccttcat aaaagagtcc aaaggcctgc ggtggtttac
     9901 catcatgctc atggatcttg ggcagaccac cgttgaaatt gtacagttgg tggaatatag
     9961 gatggctgaa aggcagttcg gtcagttcct gatcagggaa gacctttttc agtgcaggac
    10021 ggatataagg atccatcccg tagttatcat cgatgtgcag gaagcctccg gccatcagcc
    10081 acttgcgcag attctctgct tcggcatcac taaaaaccac gtttccgtga ccggtcatgt
    10141 gtgcaaaggg atagttaaaa agatcgcgac tgcccacttc taccgtagcg ggttcagagt
    10201 cgatgtccat gcctgcatat ttgtctgcat atgctaccag attacccaga gaggtcgggt
    10261 tggcatacca gtcgccgcca ccgttgtatt tcaacagggc aatgctgtat gactgtgccg
    10321 aagcagccac agagatacac aacaaaccgg cgataaaaag tcctctcata atttcccgtt
    10381 cagcaaatgt acggtaatga ccgcttgcag cgccgcggtt tcagtgcgca agcgctgttc
    10441 tcctaacgag acggggatgg ccttattttc cagagcgagt tgcagttctt ccggtgtgaa
    10501 atctccctcg gggccgatga ggatggtaca tggctgacca ggcggtacac acctgctgag
    10561 cagctgcttc tctcctgcca tgcaatgggc gaagaatacg ttttcgttct gttgtttcca
    10621 gaagtcggcg aaggacaccg gctcctgcca ttcaggcagc cagcatcttc tgctttgctt
    10681 cagtgccgac tgcatcgtgg cgatggcctt gtcgctgcga atgcttcttc tttcagaacg
    10741 atcggtgatg accgggatga tacgggagat gcctacttcg gtcagttttt ccagcagcca
    10801 tcccattctg tccatggatt tgaccggtgc aatggccagt gtgacagccg gttctatggg
    10861 ctgttcggac aaccgttcat gaaccgcaac actacaatgt tttttacctg catgagtgat
    10921 ggtagcgatg gcgaggtttc cttttccatc tgtcaattcg actgtatcac cgaattgcat
    10981 gcgcaacacc tgcatggcat gccggccttc ctgatcgggc aggtcaagca catcagattc
    11041 caatagatcg tccagtatga aacggggcgc tttgctgtcc ataggacgaa ggtaatggct
    11101 ttaaggcagg atgaccacag gccggaaacc gatgaaagga gacgcatcat cgaaaccggc
    11161 atattcatcc ggagcagtga tgcgcatctg gatggcagta cttccccagg agccgccttt
    11221 ggtgcgacct tttgtttcga gcatctcggc tacattgcca ctcatgttat agaggccgta
    11281 tgcattcggg tagtaggaac gactcaggtt ggtggccagg caggtaccgt cgtcaggtga
    11341 attatagtta cagaattcca cttctttctt tccgttcaca cccagattct cccaggggta
    11401 ttctgcatcg ttctttccgc ctttggccgc atggatccat tcgatctcat cgggcaggcg
    11461 tacgtaggca tccggcactt tctctctgat catttcctgg agatagtcgc agaatagctg
    11521 tgcagcttca tacgtgatgt tcactacggg ataatccaca aaagccggat cggtatggta
    11581 atctgcagca tacgtatttt caaatgacag ggcagtttcc cagttcccgt tgtggatgcc
    11641 ggccatctgc agatcagcct gcctgtcgtg tttcttcagg tattgcagga actcggcata
    11701 ttgtccgttg gtgatctctg cttcgtacat ataaaaacct ctgacagata cttcgcctga
    11761 atcgctgtac acgacaccgg cagggatgta atgaaagcca agaccttcca gatacttgcg
    11821 tcctttattg ttgccgaaag acaggatgac ggcagagaac aggatggtga acagggctgc
    11881 gctgaatttc actcgcagga ttttcaacaa tattattttt cttacaggat gcactcaagc
    11941 acttctttac acataattca atggaaaatc gtgccagtgt acagaataca agaataaggc
    12001 cggcgcaagc gctggaaaac gaccgttccc ggtaaatgcg tagatatagc tttagttcct
    12061 tacgatcagg aacatcctga aaaaatccga cccttctaat ttcatcatat acatacctgc
    12121 gggccataat gcggtggata atgtgatatt ctcactgaat tgcgttttgc tcaatacagc
    12181 actaccattc atatcatata cagacaaatg tccctcccgg atgtctgtgt agatggacaa
    12241 catgtcatta aaaggattag ggtacaccaa aatgctctga gcttcattac tgagataagt
    12301 acctattggc accatcaccg tatccaccac actgcacccg ttggcatctg ttacggtcag
    12361 gatgtaggtg cccggccagg tgccaatgat ggtatctcca ctcaggaagg catcattcca
    12421 cacgatagtg taaggcggtg tgccgttgga gggcaacaca atgatgttgc cacctggccc
    12481 gc
//
LOCUS       DAOMAX010000056        12394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_648_length_12394_cov_4.265175, whole genome shotgun sequence.
ACCESSION   DAOMAX010000056 DAOMAX010000000
VERSION     DAOMAX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12394
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_648_length_12394_cov_4.265175"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6165)
                     /locus_tag="PK742_04695"
     CDS             complement(<1..6165)
                     /locus_tag="PK742_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU75922.1"
                     /translation="MKKVLFFIGLLNIAMLHAQSDVTVTLVIAPPYSPYLSDYTEFGG
                     QNILQLTNTTSATLQLKLVGQIEGLDNGIQVSTDVGYQPVAPVTLAPFQTLFVTGATQ
                     SMDFLDANHITTNLTSDQEYSLFTSGILPEGMYTICIRALDYNTGTPLSADAPSGCTT
                     ITIAYPTPPILINPICSTAINMNLPVFTWTPVISSGSFFFYDLYVMELLEGQIPEDAM
                     YLAIETGLGNPVVIHNLATTTYNYKPFDVPLVAGKTYIWCVVARDANNLIQIVNDGRS
                     EVCMFTYEEPYVPPLVEEVEQDLLTETIFSLNNSSVTGQLMYRFYGNEEFGMPPAPVY
                     NPTLTVYDIPKSAEELALMQPQNDNADGNSGNGYQMSGTGLFDDFFVSEVSTALGLIG
                     GKIDIDPGVKYLYQNTLNSGGAAPLRNTSVSIYLEYVAVVRNIAGEEYYFPVIPGAEL
                     YFNATAVNAHGNPIDPQLAYAASQAGLNVDDAMALRPLGSELLGTATTDENGNFTFNF
                     DMTENTGLLQAGPLTLLTWYFNPPEIPNEEHEWVNPLDEVSNPWDALLNPVDELMNDP
                     YDALFGGNMFMQDNGGWNASGSGTEGFTTDFNFGNTFNNLFNSNGLNQNFNQNFNQFG
                     PGPAPFDPPVLSAWDLLTYQNYTVTNLFKVLRIKVNDPKYVSPDVLIFVQPGDHVTLP
                     PVTTFVNSYDAHITFIAGGKESDDPLLAPGVPMSNVNVRIGHLKSWWDTRPNNFPAGE
                     GMDINPDEFFSVDPGTPYFYSWNQDEDKPGQLRLTSELPTTASGKAIFSRLVGNINYT
                     NSVFYFEAQNALTGTYNYTGSWGSFWKTVYLPPTMNEAVHSWNFRPDTIEKEVKLEPL
                     DPELLLRTVTVSNIETAPLEGVDVYMLEYNKAGSTYTYTGFDLGETDAEGYQRFTNID
                     MIYGPSGLENPYRRVMLNKWGYAIKYLPVNVSTPTADYHYLQPAKKGQRLDLGEVVMT
                     GNAGVYGYVKDDLDKPVAALVRIGDGPWYMTGMTMDDTNYDSPVISSVPVEHPPVYFS
                     LPLGDLYSSIDFFNSGDYMTSDDILSDIQVVDLSGMGTQYNDGGFQIVDQMPEFGWAD
                     NGILDNMLDMKSKFTINTGGTGSNTRVIIVPMSDQYMADTFYVNVPANVSDHNLGTFV
                     VFEKAHRVKVQVKGDNGNGLFGPLPLIGASVEVGDYGGGTGQDGFAWFRFTTPDSYFR
                     MYMQANGYVPFENYEYIPISKSYQIKTYTLEQGMTVTGQVINAVTGAGIENARVFVPT
                     GTTLYGLTDVETYTDANGFYTLQGVPREVIDIHAAVSSSDPSYIGNTYHVYFPGPAIS
                     HANIALQPIGSMDLGNLYGFPIEIDSYLAQMNGHAIISGAFVDVPSNANFSMLDENTR
                     IRFVNIEVAPDGPPGAGGLPTAVAVAGEVNTLELVTRVRVHEAFVADMFSHKTGWFPT
                     IAVQPADEGEGTIKGKVNMDLESFKFSYDYSGSFYLGETSSSATVNAFLADPAASPER
                     SYYLMNLKYGVPTDISFTIHDFDAKADRTRSKLSNDSVLIATLLYPDLPLAGDVEVDA
                     GTISVTPTSIVIGNPGEEISFGLEDWTVESDGGWSYSIPLGGIVMPSASIQTQLVDIP
                     ISNLILRPTELIMEAGNVNLDDLMVGGNVTKLYQYTGSEAVFNFDPACSWDLGPHWRF
                     SLYGENDGPSSYIKGLPGFVSTQHIDFGSFTLYSNNSTYIQPITQVLPFHNISTISIQ
                     NISSGVDGLEIAGAFFLDIPDMTTPTIILEYTKPSYAIVRKVKSLDIGLQNPGKVYFD
                     GYLGTEYYTISDNYFEANGELIFEDDNLNDNNKIELKGKLVKDLTGTVLSIPKLQNNN
                     TDAAYQYIQMDGGYQSKLKVLQGEQKVVGGAWNTMWFEADLVGVEGMGNDKTLKYLVS
                     GAVEADSDPSNKLEIEDIQTPFGGLTLVFDWQKGSFLGSLTISVPIVIYSFQLNSGLF
                     ELYVDGTGFYFDVIGNVSLPGLEWLDVNVGCLTGYYEELPDAVIDRHANIMYLADVPD
                     YLINDGIGGIYFNANVAPSIANWSASIDLLVFAVGMGVN"
     gene            complement(6168..7895)
                     /locus_tag="PK742_04700"
     CDS             complement(6168..7895)
                     /locus_tag="PK742_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75923.1"
                     /translation="MQVPALHLVVKLLGASLCFLLLSRSEIILAQEADSAAANDKKLH
                     FGSGITMSAQFYHMDGATARQQPFMYAISGTPSLSYGNVAVPFTLLYSNQVLSFQQPF
                     NRFGISPRFDWGTIYLGSSSMRFSNYTLAGQRFDGAGADLQLGILRIGAMYGRFSREV
                     RPDDAVDDPETFINEAATASFLRTGYAAKFGLGTRESYVDFVYLNAADQQPAEYDYSE
                     WEMHPAQNVVLGIRSVINAKGLVVITNDWAASAYTRDQFADSIEIEDAFIRSVTKNLL
                     TPNQSTQLRFAGESAITLKTKYVSPGLLYKRIELDYKTMGAYYFLNDIEQYQANLSFR
                     FLQNKLTGSGSFGQQHDNLQDHHLSTSRRNIGALSLNYTPSAVWGVSGSFSNFGITTN
                     PLPTSLADTTRLKQVSNTFSLVPRYQLLKGKHNHLFTLVSSYSALNNLGDPIGEAAET
                     TSLTNSLSYSWTWVPYSFQTGCTPTFIRTTTILGEYQATGVSLQASKAFLAGKVSSGI
                     QTGVFANSFNQVSNGRTVTAGIQGSVNWPKWPGASLGLQYMNNQSADETVLASFRELY
                     ANISINYNF"
     gene            complement(7963..8331)
                     /locus_tag="PK742_04705"
     CDS             complement(7963..8331)
                     /locus_tag="PK742_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQU75924.1"
                     /translation="MQEINKISPDALLEMVREPWSPVIIAGLNGQELKIARFRGEFVW
                     HLHEHEDEAFMVIEGAFDMHFRNKVVHLEKGEIIVVPKGTEHKPVAEQDAVVLLFEPI
                     GTINTGNTGGPFTREAEPVP"
     gene            complement(8319..9083)
                     /locus_tag="PK742_04710"
     CDS             complement(8319..9083)
                     /locus_tag="PK742_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQU75925.1"
                     /translation="MPFLVLIWFSSLFAFSDSDSPVFTDPVAKEADNMVLKHFPDTAA
                     ARHFLLGRFDPAKHPDFIRLTEQDAIKDFYLQKETAAAFRKMQAAAADDGISLKIVSG
                     GRTFQDQKNIWEWKWKEQKKAGRKDSLDIATRILEYSLMPGTSRHHWGTDIDINNINN
                     SYFQTSRGMKVYEWLTTHAGEYGFCQVYADESGRPGCAIEKWHWSYLPLAETYLQIYN
                     SVITYQDLDQFSGSGQADDLEVISRYVNGISTACRK"
     gene            complement(9165..9719)
                     /locus_tag="PK742_04715"
     CDS             complement(9165..9719)
                     /locus_tag="PK742_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75926.1"
                     /translation="MKVLVVSLMTAICCISAMQAQIPDAAERTGQGVPSAMHHIWLSD
                     TAGQQIYYGQLLHASDSGVVVTDRRGYGTDTLFYAYEHLGLIRCRKSSSFFYKALAGA
                     GIGMLTGVAVGYLSSQDDNPGDLLDLSGLLPFMYGVVGFEVGGLIGFLTGFYKARWLP
                     EGRAVLTSEELKQLQEYQTRYWSR"
     gene            complement(9719..10345)
                     /gene="rsmG"
                     /locus_tag="PK742_04720"
     CDS             complement(9719..10345)
                     /gene="rsmG"
                     /locus_tag="PK742_04720"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQU75927.1"
                     /translation="MDRILKHFPDLDKRQISQFRQLGPLYSYWNQKVNVVSRQDIDNL
                     YEHHVLHSLSVARFIQFQPGSKVLDLGTGGGFPGIPLAIMFPEVHFHLIDSIDKKVKV
                     AEAVAKAIGLRNVTTEQVRAEDHKAQYDFVVSRAVADLMIIYGWVINRIRRGSSFNDV
                     PNGLLCLKGGNLDMEMAPFKDLIYKFPVKDFFNEVWFADKYLLYLPME"
     gene            complement(10387..11601)
                     /locus_tag="PK742_04725"
     CDS             complement(10387..11601)
                     /locus_tag="PK742_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQU75928.1"
                     /translation="MRTYLSRRDFLRNTSLASASLFIPRFLRASGTMSFADAGKPKIL
                     VVIQLSGGNDGLNTVVPFRNDIYYEKRPVIGLNPDELIKIADDTGLHPALSGLADLYH
                     NGDAVIINSVGYPNPNRSHFRSMDIWQSGSDPNQYLHTGWLGRVLDATCTNDCVPPHF
                     AVEVDDSLSLALKGETISGFAVRDPRTLKKTGVNPFIDELAEGYTGEGDIHDQVVYLH
                     KVLSDAYESTGYLQEQAKIYSSQTEYPLTDLGKQLKAVAELIISGSETRIYYVSLSGF
                     DTHAGQRGQQDRQLRVYSDALRAFCQDLKSNHVFQDTLVLTFSEFGRRVEQNASNGTD
                     HGTANNVFVVGGGLRKAGIVNELPDLTDLDDGDLKYQTDFRSIYATILDRWLMADAGN
                     ILGGDFGYIDFL"
     gene            complement(11603..>12394)
                     /locus_tag="PK742_04730"
     CDS             complement(11603..>12394)
                     /locus_tag="PK742_04730"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HQU75929.1"
                     /translation="YEFDEKEHDDGEKTVLGRTGNWNGNDIIDMLLEDRQTARYVCEK
                     IYRFFIGEQVDRSWLDELSDQFYNSNYDISKLMRSIFLDDRFYSRDVQGSLIASPVEL
                     IVRYKRLISLETKNDNDTLKLQKVLGQVLFFPPNVAGWPGGRNWIDASSLRLRMDLPR
                     TIMEGGAFDLRPKPDFEDAPIAEGQSKTDKKASVRSDWNTLLRYFRKTKREELTKKVL
                     DTFIQSDYDERLVALIDKWVDHSSADKQIISTCAAVFSIPEFQLI"
BASE COUNT         3180 a         3072 c         2904 g         3238 t
ORIGIN      
        1 attcacaccc atacccactg cgaatacaag caggtcgata ctggcactcc agttagctat
       61 ggaaggagca acattcgcat tgaaataaat accgccaatt ccatcgttga tcagataatc
      121 aggtacatct gccagataca tgatattggc gtggcgatca attactgcat caggcagctc
      181 ttcgtaataa ccggtaagac aacccacatt cacatccagc cattccagac ccggcaggct
      241 cacattgcct attacatcaa aatagaatcc ggtaccatcc acatacaatt cgaacaggcc
      301 gctgttcagc tggaagctgt agatcacgat gggaacagag atggtcagtg atccgaggaa
      361 ggaacctttc tgccagtcga acaccagagt caatccgcca aaaggcgtct gaatgtcttc
      421 gatctccagt ttgttggatg gatcggagtc agcttccact gcaccactca ccaggtattt
      481 cagggtctta tcattcccca taccttccac accaaccagg tcagcttcga accacatagt
      541 gttccatgcg ccacctacaa ctttctgttc gccctgcagc actttcagtt tgctctggta
      601 acctccgtcc atctggatat actgataggc cgcatcggta ttgttgttct gcagtttagg
      661 aatgctcagc accgtgcctg tcagatcctt gaccagtttt cctttcagtt cgatcttgtt
      721 gttgtcgttg aggttatcat cttcaaagat cagttcacca ttggcttcaa aataattgtc
      781 ggaaatggta taatattcag tacccaggta tccgtcaaaa tagaccttac ccggattctg
      841 taatccgata tccagagact tcaccttccg cacaatggca taagaaggtt tggtgtattc
      901 caggatgatg gtaggggtgg tcatatcagg aatatccagg aagaaagcac cggcaatttc
      961 cagtccgtcc accccgctgc tgatattttg tatgctgata gtagaaatgt tatggaacgg
     1021 cagcacctgg gtaatgggct gaatgtaggt actattgtta ctgtacaggg taaatgaacc
     1081 gaagtcgatg tgctgggtac ttacaaagcc cggcaagcct ttgatatagc tggagggccc
     1141 gtcgttttca ccatagagac tgaagcgcca gtgcggaccg aggtcccagc tgcaggcagg
     1201 atcgaaattg aatactgctt cagatccggt gtactgatag agtttggtca cattacctcc
     1261 caccatcagg tcatccaggt ttacgttgcc tgcttccatg atgagttcag tgggcctcag
     1321 gatcaggttg ctgataggta tatcaaccag ttgtgtctga atgctggccg atggcataac
     1381 gataccaccc agtgggatgc tgtaagacca gccgccatca ctttcaacgg tccagtcttc
     1441 caggccgaaa gagatctctt cacccggatt acctatcaca atagatgtag gtgtaacact
     1501 gatggtaccg gcatccactt ctacatcgcc ggccaggggc agatcaggat acagcagggt
     1561 ggcaatcagt acggaatcat tggaaagttt actcctggtt ctgtcggctt ttgcatcaaa
     1621 atcgtggatg gtgaaactga tatctgtggg caccccgtat ttcaggttca tcagataata
     1681 gcttctttca ggagaggcag caggatctgc caggaaagca ttgaccgttg cagaagatga
     1741 agtttcaccc agatagaagg aaccggaata gtcataagag aacttaaagg attcgaggtc
     1801 catattgacc tttcctttga tagtgccttc tccttcatca gccggttgta cagcgatggt
     1861 agggaaccat cctgttttat ggctgaacat atcagcaaca aaggcttcat gtacccttac
     1921 ccttgtaacc agttccagtg tattcacctc accggccacc gctacggcag tgggtaaacc
     1981 tccggcaccg ggcgggccat caggtgccac ctcaatgttg acgaatctga tccgggtgtt
     2041 ttcatccagc atagagaaat tggcattgga aggcacatct acaaatgcac cactgataat
     2101 ggcatgtccg ttcatctgcg ccaggtatga gtcgatctct atcgggaaac catacagatt
     2161 acccaggtcc atactaccaa ttggctgtag tgcaatattg gcatgagaaa tggcagggcc
     2221 ggggaaatag acatgatagg tgttacctat atacgatgga tcagaactgc tcactgcggc
     2281 atggatatcg atgacctctc tgggcactcc ctgcagggta tagaatccgt tggcatctgt
     2341 ataggtctct acatcagtaa gtccgtacag ggtggtgccg gttggaacaa atacacgtgc
     2401 gttttctatt cctgcaccgg ttactgcatt gattacctga cctgtaacgg tcataccctg
     2461 ctccagggta taggttttta tctgatacga tttagaaata ggtatatact catagttttc
     2521 aaaaggcaca tacccattag cctgcatata cattctgaaa tagctgtcag gtgtagtgaa
     2581 ccggaaccat gcaaatccat cctgtcctgt tcccccgccg tagtcgccca cttcaacaga
     2641 agcacctatc agcggtaaag gaccaaacag gccatttccg ttatcacctt tcacctgcac
     2701 ttttacacgg tgagcttttt caaatacgac aaatgttccg aggttatgat ctgaaacatt
     2761 ggcaggcaca ttcacataaa aagtatcagc catgtactga tcagacatcg gtactatgat
     2821 gacccgtgta ttgcttccag ttccacctgt attgatggtg aacttagact tcatgtcgag
     2881 catattatcc agtatgccgt tgtctgccca tccgaactcc ggcatctgat caactatctg
     2941 aaagcctccg tcgttgtact gtgttcccat acctgaaaga tccaccacct ggatatcaga
     3001 caggatatca tcgctggtca tgtaatcgcc ggagttgaaa aagtctatag aagaatacag
     3061 atcacccaac ggcaaactga aatacaccgg tggatgttca acgggcacac tgcttataac
     3121 cgggctgtca tagttggtat catccatggt cataccggtc atataccagg ggccatcgcc
     3181 gatcctgacc aaagcagcca caggtttatc cagatcatct ttcacatatc catagacacc
     3241 tgcgttaccg gtcatgacca cttcgcccag atcgaggcgt tgtcctttct ttgccggttg
     3301 cagatagtgg tagtcagccg tgggtgtgct tacattaacg ggcaggtatt tgatggcata
     3361 gccccatttg ttcagcatga cccgtctgta cggattctcc agaccactgg gtccatagat
     3421 catatctatg ttggtaaaac gctggtaccc ttctgcatcc gtttctccca gatcaaaccc
     3481 ggtgtatgta taggtggagc ctgctttgtt gtactccagc atatagacat ctactccctc
     3541 cagcggtgcg gtctcaatat ttgaaacggt tacagtgcgc agcagcaatt ccggatccag
     3601 aggttccagt tttacttcct tttctatcgt gtctggtctg aaattccagg agtgaacggc
     3661 ttcattcatc gttggcggca gataaacagt tttccagaag cttccccagc tgccggtata
     3721 gttataggta ccggtcagag cattctgcgc ctcaaaatag aatacactgt tggtatagtt
     3781 gatattgcca accagtctgg agaaaatggc cttgcctgaa gcggttgtag gcagttcact
     3841 tgtaagccga agctgaccgg gcttgtcttc atcctgattc cagctgtaga aataaggtgt
     3901 acccgggtcc acagagaaga attcatcagg attaatatcc attccttcgc cagcagggaa
     3961 gttattgggt cgggtgtccc accaggattt caggtggcct atgcgcacat tgacattgct
     4021 cataggtacg ccaggcgcca gcaacggatc atcactttcc ttaccacctg caatgaacgt
     4081 gatatgtgca tcataagaat tcacaaatgt ggtgacgggg ggaagggtca catgatctcc
     4141 gggctgcacg aatatcaaaa catcaggaga tacatacttg gggtcattca ctttgatgcg
     4201 gagcaccttg aacaggtttg ttaccgtata attctggtaa gtcagcagat cccaggcaga
     4261 gagaacaggt ggatcaaacg gagctgggcc gggaccgaat tggttgaaat tctgattaaa
     4321 attctgattc agcccgttgg aattgaacag gttgttaaac gtatttccaa aattaaaatc
     4381 tgttgtaaat ccctccgtac ctgaaccgga tgcattccat ccgccattgt cctgcatgaa
     4441 catattgccc ccgaacaggg cgtcataagg atcattcatc agttcatcaa ccgggttcag
     4501 caatgcatcc cacggattgc ttacttcatc cagcggattg acccattcat gttcttcatt
     4561 gggtatttcg ggcggattga aataccaggt gagcaatgtg agcggaccgg cctgtaacag
     4621 gccggtgttt tcggtcatat caaaattgaa ggtaaagttg ccgttctcat cggtggtagc
     4681 agtacccagc aattcagaac caagcggtct cagggccatg gcgtcatcca cattcagacc
     4741 agcctgactt gctgcgtatg ccagctgcgg atcgatgggg tttccatgtg catttacagc
     4801 cgttgcattg aagtatagct cagcgccggg aatgaccggg aaataatatt cctcacctgc
     4861 tatatttctg accacggcta catactccag gtaaatggac acagatgtat ttcgcaaagg
     4921 tgctgcgcca ccgctgttca gtgtattctg atacaggtat ttcacacctg gatcaatatc
     4981 gatcttgccc cctatcaggc caagtgcggt ggatacttca gaaacaaaaa aatcatcgaa
     5041 cagacctgtt cctgacatct ggtaaccatt tccggaattg ccatcggcat tgtcattctg
     5101 cggttgcatc agcgccagct cttcagcaga tttcggaata tcataaactg tcagggtagg
     5161 attgtacaca ggtgcaggcg gcatgccaaa ctcttcgttg ccatagaagc ggtacatgag
     5221 ctgaccggtt acagaagagt tgttgagact aaagatcgtt tcggttagca ggtcttgttc
     5281 cacttcttca accaaaggcg gtacataagg ttcttcatag gtgaacatgc aaacctcact
     5341 tctgccatcg ttgacgatct ggatcaggtt gttcgcatct ctggccacta cacaccatat
     5401 gtaggtctta ccggcaacca gcggcacatc aaacggctta tagttatacg tagtagtagc
     5461 caggttgtga atgaccaccg gattacccag gcctgtttca atagccaggt acatggcatc
     5521 ttcgggtatc tgaccttcca gcaattccat tacatacaga tcataaaaga aaaatgaacc
     5581 ggatgaaatg accggtgtcc aggtgaaaac cgggaggttc atattaatgg cagtactgca
     5641 gatcgggttg atcagaatgg gaggtgttgg ataggcaatg gttatggttg tacatcctga
     5701 aggtgcatct gcagacaggg gtgtgccggt gttataatcc agtgctctga tacagatggt
     5761 gtacatgcct tcgggcagga tgccgctggt aaacaggcta tattcctgat cggatgtcag
     5821 gttggtggtg atgtggttgg catccagaaa atccatagac tgggtagcac cggtaacgaa
     5881 cagcgtctga aacggagcca gtgttacagg agcaaccggc tggtagccaa catcagtaga
     5941 aacctgaata ccgttatcca gtccttctat ctgacccacc agtttcagct gaagggtagc
     6001 agaagtggta ttggtcagct ggagaatatt ctgcccgcca aattccgtgt agtctgacaa
     6061 gtagggacta tagggtggtg cgatcaccag tgtaacggtc acatcgcttt gggcatgcag
     6121 catagcgata ttcagcaaac cgataaaaaa caataccttt ttcatgatca gaaattgtag
     6181 ttgatactga tattggcgta caattcacgg aaagatgcca gtacggtttc atccgcactt
     6241 tgattattca tatactgtaa tccaagagat gccccgggcc acttcggcca gttgaccgaa
     6301 ccctggatcc cggcggtaac cgttcgtccg ttggaaacct ggttgaatga gttggcaaaa
     6361 acaccggtct gtattccact gctgaccttt ccggccagga atgcctttga tgcctgcaga
     6421 gaaacacccg tagcctgata ctctcccagt atagtagtgg ttcggatgaa agttggcgta
     6481 catcctgtct ggaaactata aggcacccag gtccacgaat aactcagact gttggtcagg
     6541 gaagtggtct ctgcagcctc gccgatagga tctccgaggt tattgagtgc tgaataagaa
     6601 gaaaccaggg tgaacaggtg attgtgttta cctttcagca gctggtacct aggaacaagg
     6661 ctgaaagtat tacttacctg cttgaggcgg gtagtatctg ccaggctggt aggaagtgga
     6721 ttggtggtga tgccgaagtt ggaaaaactg ccggatactc cccaaacggc cgatggtgta
     6781 tagttgagac tcagcgcacc tatatttcgt ctggatgtgg acaggtggtg atcctggagg
     6841 ttatcatgtt gttgaccaaa tgaaccgcta cctgttagct tgttttgcag aaaacggaaa
     6901 ctgagattag cctgatactg ctctatatca ttcagaaaat agtaagctcc catggtcttg
     6961 taatccagtt caattcgctt atacaacagg ccgggcgaaa cgtatttggt tttcagcgtg
     7021 atggctgact cgccggcaaa ccggagctgg gtactttgat tcggggtgag cagattttta
     7081 gtgaccgagc gaataaaggc gtcttcaatt tcaatactgt cagcaaactg atcgcgggta
     7141 taggcactcg ctgcccagtc gttggtgatg accaccaaac cctttgcatt gatgaccgat
     7201 ctgattccga gtacaacatt ctgagccggg tgcatttccc attcactgta atcgtactca
     7261 gccggttgct gatctgcggc atttaaataa acaaagtcca catagctctc tctggttccg
     7321 aggccgaact tggcagcata gcctgttctc agaaaactgg cggtagctgc ttcgttaatg
     7381 aaggtttccg gatcatctac tgcatcatcc ggtcttactt ctctgctaaa ccttccatac
     7441 atggcaccaa tgcgcagaat gcccagttgc agatctgcgc ctgccccatc aaagcgctga
     7501 ccggccaggg tgtaattgga aaaacgcatg gaggaagagc ccaggtaaat ggtaccccag
     7561 tcaaacctcg gactgatgcc aaaccggtta aatggttgct gaaaactcag tacctgattg
     7621 ctgtacagta aagtgaaagg taccgccaca ttgccgtagc tcagtgaggg tgttccgctg
     7681 atagcataca taaaaggttg ctgacgggcg gtggctccat ccatgtgata gaactgagca
     7741 gacatggtga taccggaacc aaaatggagt ttcttatcgt tcgcagcagc actgtcagct
     7801 tcctgggcaa gtatgatttc tgacctgctg aggagcagga aacacaggga tgccccgagc
     7861 aatttaacaa ccaggtggag ggctggcacc tgcatagggc attctttagg ttttcccaaa
     7921 tatacccagt tgtaacaaca aaagcaatga tcagaaaaaa attcagggta ccggttctgc
     7981 ctccctggta aacggaccgc cggtattacc tgtattaatg gtgccaatgg gctcaaaaag
     8041 gagtaccaca gcatcctgtt cggccaccgg tttatgttct gttcctttgg gtaccacgat
     8101 gatctcacct ttttccaggt ggaccacttt attgcgaaag tgcatgtcaa aagcgccttc
     8161 tataaccata aacgcctcat cttcatgttc atgcaaatgc cagacgaatt cgccgcgaaa
     8221 cctggcaatt ttcagttcct gtccgttcag ccctgcaatg atcacaggcg accatggttc
     8281 cctgaccatt tccagcagtg catccggact gatcttgttt atttcctgca tgcagttgaa
     8341 attccgttca catacctgct gataacctcg aggtcatctg cctgcccgct tccgctgaac
     8401 tgatccaggt cctggtaggt gatcacacta ttgtagatct gtaggtaggt ttcagccagt
     8461 ggcagataac tccagtgcca tttttcaatc gcacatccgg gtcttcctga ttcatctgca
     8521 tacacctggc agaatccgta ttcgccggca tgggtagtaa gccattcata gaccttcatt
     8581 ccacgactgg tttgaaagta tgaattattg atattgttta tatcaatgtc cgtcccccag
     8641 tgatgcctgc tggtacccgg catcaggctg tattccagga tacgagtggc aatgtcaagg
     8701 ctgtcttttc tgcctgcttt cttttgttct ttccatttcc attcccagat atttttctgg
     8761 tcctgaaagg ttcttcctcc acttacgatc ttcagagata tgccatcatc ggctgctgcg
     8821 gcctgcatct tccggaaagc agctgcagtt tctttctgta aataaaaatc cttgatggca
     8881 tcctgttctg ttagccgaat aaaatcagga tgcttagccg gatcgaacct gcccagcaga
     8941 aagtgccgtg ctgcagccgt atcaggaaaa tgcttcagca ccatattatc tgcttctttg
     9001 gcaacaggat ctgtaaatac aggactatcg ctgtcagaga aagcgaacag gctgctgaac
     9061 catatgagta ccaagaatgg cataaggggg catgaaaata cagcataagt ggtgaccccg
     9121 caacagcaga tccaaactat taggccagaa atgttacgtg caacttaacg gctccagtat
     9181 ctggtctggt attcctgcag ttgtttcagt tcttcactgg tgagcacagc acgtccttcc
     9241 ggaagccatc ttgctttgta aaacccggtc aggaaaccta tcagaccacc cacctcaaag
     9301 cctactactc catacatgaa aggcagcaag cctgacagat ccagcaggtc tccggggtta
     9361 tcatcctgag aagaaagata tccaacagct accccggtaa gcataccgat acccgcccct
     9421 gccagggcct tgtagaaaaa ggaagaactt tttctgcacc ttatcagacc caggtgttca
     9481 taggcataga agagggtatc cgttccatat cctcttctgt ctgtcacaac cacacctgag
     9541 tcagatgcat gaagcaattg cccgtaatag atctgttgtc cggcagtatc agaaagccag
     9601 atgtgatgca ttgcagacgg gacaccctga cctgtgcgct ccgcagcatc cggaatctgg
     9661 gcctgcatag ctgaaataca gcaaatggct gtcatgagtg agacaactaa gaccttcatc
     9721 attccattgg caggtagagc agatacttat cagcaaacca aacttcattg aagaaatcct
     9781 ttacaggaaa tttatatatc aggtctttaa atggagccat ctccatgtcc agattacctc
     9841 ctttgagaca aaggaggcca ttgggcacat cgttaaaact actgcctcgt ctgatgcggt
     9901 taatgaccca gccataaatg atcatcaggt ctgccactgc cctgctcacc acaaagtcgt
     9961 actgcgcttt gtggtcttcg gcccgtacct gttctgtggt cacgttgcgc agaccgatgg
    10021 ctttggcaac tgcttctgcc actttcacct tcttgtcaat ggagtcaatc aggtgaaaat
    10081 gaacttcagg aaacatgatg gccagcggaa tgcccggaaa accgccgccg gtacccaggt
    10141 ccagcacctt gctgcccggc tggaattgta tgaacctggc tacagagaga gaatgcagaa
    10201 catggtgctc ataaaggtta tcaatgtcct gtctggaaac aacattgacc ttctggttcc
    10261 agtaactgta caagggaccc aattgcctaa actgggatat ctggcgcttg tccagatcag
    10321 ggaaatgttt caggatacgg tccacctaac aattttatcc attaatacgc agttttccca
    10381 atcttattac aaaaagtcaa tatatccgaa atcgccaccc agaatattcc ctgcatctgc
    10441 catcagccac ctgtccagaa tggtagcata gatgcttcga aaatccgtct gatacttcag
    10501 gtcgccgtca tccagatctg tcagatcagg caattcgttc acaatgcctg cttttctcaa
    10561 acctccgccc accacgaaca cattattggc agttccgtga tcggttccgt tgcttgcatt
    10621 ctgttccacc cgtcttccaa attcactgaa tgtaagaaca agtgtatcct ggaaaacatg
    10681 attagacttc agatcctggc agaaggccct cagagcatct gaatagaccc gaagttgcct
    10741 gtcctgctga ccgcgttggc cggcatgggt gtcaaatccg gacaatgata catagtagat
    10801 cctggtttca cttccgctta tgatcagttc agccactgct ttgagttgtt ttcccagatc
    10861 ggtaagcgga tactctgtct gggaagaata gatcttcgcc tgctcctgga ggtagccggt
    10921 gctttcataa gcatcactga gtactttgtg caggtaaacg acctgatcgt gaatatcgcc
    10981 ttcaccggtg taaccttctg ccagttcatc aataaaagga ttgactccgg tttttttgag
    11041 tgttctggga tcccggactg caaacccgct gatggtctct cctttcaatg ccagactcag
    11101 gctgtcatcc acctctacgg caaaatgagg tggtacacaa tcgttcgtac aggtagcatc
    11161 caataccctg cccagccagc ctgtatgcag gtactggttt gggtcactgc cactctgcca
    11221 gatatccatg ctgcgaaaat gcgatctatt ggggttgggg taacccacac tgttgatgat
    11281 gactgcatct ccattatggt acagatcagc caaaccggat aaggccggat gtaagcctgt
    11341 atcatcagct atcttgatga gttcatcggg attcagtccg atgaccggac gtttttcata
    11401 atagatgtca tttcggaacg gcaccacggt attgagtccg tcgtttcctc cggacagctg
    11461 tatcacaacc aggatcttag gtttccccgc atcggcaaaa gacatggtgc cactggcacg
    11521 caggaaccgt ggaatgaaca gcgaggcact ggccaggctg gtgtttctga gaaaatctct
    11581 tcttgacagg tatgtacgca tatcagatca gttgaaattc aggaatggag aagaccgcag
    11641 cacaggtact gatgatctgc ttatcggctg aagaatgatc gacccactta tcaatgagtg
    11701 caacaagtct ttcatcatag tcagactgga tgaaagtatc gagtactttc tttgtcagct
    11761 cctcccgttt ggtcttgcgg aaatacctga gcagcgtatt ccagtcgctg cgcacactgg
    11821 ctttcttatc tgtcttactc tggccttcag caataggagc atcttcaaag tcaggtttcg
    11881 ggcgcaggtc aaatgcaccg ccttccatga tggttcgggg caggtccata cgcaggcgca
    11941 gtgaactggc gtctatccag ttcctgccac ccggccaacc tgcaacgttc ggcggaaaga
    12001 acaacacctg ccctaatact ttctgtagtt tcagggtgtc gttgtcattt ttggtctcga
    12061 ggctgataag gcgtttgtag cgaacgatca attcaaccgg agaagcgatc agcgaaccct
    12121 gcacatcccg gctgtaaaac cgatcatcga gaaaaatgga acgcatgagc ttgctgatat
    12181 catagttact gttatagaac tgatcactta actcatcgag ccagcttctg tcaacctgtt
    12241 ctccgataaa gaaccgatag atcttttcac aaacataccg ggctgtttgc ctgtcttcca
    12301 gcagcatatc aatgatgtca tttccattcc agttaccagt tcttcccaac acggtctttt
    12361 ctccgtcatc atgttctttc tcgtcaaatt cata
//
LOCUS       DAOMAX010000057        12118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_671_length_12118_cov_4.485534, whole genome shotgun sequence.
ACCESSION   DAOMAX010000057 DAOMAX010000000
VERSION     DAOMAX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_12118_cov_4.485534"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK742_04735"
     CDS             <1..271
                     /locus_tag="PK742_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75930.1"
                     /translation="NLTDYEVKGSDMSVSLLMGLGRQWTFRDIVTLDLWFALGYGGNW
                     VNADTDNLPEENYYNPDQSFKYSYVRFGDSPMIFDGGLSVGILLK"
     gene            complement(533..1141)
                     /locus_tag="PK742_04740"
     CDS             complement(533..1141)
                     /locus_tag="PK742_04740"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQU75931.1"
                     /translation="MAFTLPALPYAFDALEPHIDARTMEIHHDKHHAAYTNNLNNAIA
                     GTEWEGKSIEEILANVGKISGAVRNNGGGFFNHNLFWEVMAPNAGGAPSGDLAAAIDR
                     DLGGFDKFKEAFSNAAATRFGSGWAWLIVTGEKKLMVTSTPNQDNTLMDIAEVKGTPI
                     LGLDVWEHAYYLNYQNRRPDYIGAFWNVINWNKVAANFAAAM"
     gene            1537..1980
                     /locus_tag="PK742_04745"
     CDS             1537..1980
                     /locus_tag="PK742_04745"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQU75932.1"
                     /translation="MGILTDPDSYFMQQAIRLAEQALEQDEVPVGAVVVSNGHIIARA
                     HNLVERLHDPTAHAEMQAITAACNHFGSKYLTECTLYVTLEPCTMCATAIYWAQVGRL
                     VYGASDPKLGFTRHQHIMHPKTQILGGIEEDTCGQLLKDFFKGKR"
     gene            complement(2381..4243)
                     /locus_tag="PK742_04750"
     CDS             complement(2381..4243)
                     /locus_tag="PK742_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75933.1"
                     /translation="MKRIHYLSALMAAGLLYAGNAVAQDPVCAIPGATLCDNMDSYTA
                     GDALGPGASWWTTWSGAEGGSEDGEVTDLYAYSAPNSMTIEEGGLNDVILKLGNQSSG
                     YWRLEWQMLVPEGKSGYYNIQESETPGIAWNTELVFALVDYVTPAPDGTGNVLIPGAA
                     EFSYPVGEWFKVEHLIDLDADNIEIWIDGVMVYTGAYTGNIGSVDFYSIDADCRYYID
                     DVLLTDMMPVTGTGAYYINSNTTGGEPWFSSSNLDALAANFGEGYTHEYFETVDVDAA
                     FSSENCFVFLEGSDSHADELEAFLDANIDAIEAWVASGGHLLLNAAPNEGDGMSFGFD
                     GTSLVYAYFTDNAEAADPGHPIFAGPFTPVGTNWTGTSFGHAVVTGTDLNPLIWDEFS
                     TDNWVLAEKAWGAGTVLFGGMTTNNFHSPSVEAANLRANIISYLSCTAPPVCNTPGAP
                     SVEIISSTYASVTWEPVEGVERYVFAIRDNTTGERWNRQTTDTFMELTALEPGHSYTV
                     RLRSVCYPSGFSAPGPQTDFTMPLRIGDVSNSMTIYPNPNNGQFNLLLSGYDAAQVMV
                     NITNTVGQVVFTASIAVDGDNTVQEINLENMAAGTYFVHLTDGQRNDVQTLVIK"
     gene            complement(4373..4612)
                     /locus_tag="PK742_04755"
     CDS             complement(4373..4612)
                     /locus_tag="PK742_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75934.1"
                     /translation="MIKTLTQDHLIRFMYGETGPEETGLIRESLLTDTDLHDQFEQLR
                     DAARGLDTVMASPPEYTVKRILRYSKSTAPTEHMM"
     gene            complement(4686..5264)
                     /locus_tag="PK742_04760"
     CDS             complement(4686..5264)
                     /locus_tag="PK742_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU75935.1"
                     /translation="MQVQSMPDKQLVRLYISGDEHALSELITRHRDKIYTSILFTVKE
                     AALAEDIFQDTFVKVIDTLRSGRYREQDKFLPWVMRIAYNLCIDHFRRNKRMPIVTTG
                     DGYDIFDVLGFKDKSIEENLIHDNTYDKVRNLVNHLPEEQRQVLILRHYSGMSFKEIA
                     DMTGVSINTALGRMRYALINIRKMMAEKNISI"
     gene            5494..8307
                     /gene="uvrA"
                     /locus_tag="PK742_04765"
     CDS             5494..8307
                     /gene="uvrA"
                     /locus_tag="PK742_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQU75936.1"
                     /translation="MAVGKKKSEKLENKKEQYSGIFIKGAREHNLQNIDVTIPRNQLV
                     VVTGVSGSGKSSLTIDTLYAEGQRRYVESLSSYARQFLMRLNKPDVDYIRGICPAIAL
                     DQKTTTRTTRSTVGTLTEIYDYLRLLFARVGKTFSPVSGEEVRKHSVTDVVDHFSSIA
                     AGKKVFMTVPLPRQKGRSLTETVHVLHQKGYNRLLVDGDVVKMEAVLDGEAELKAKQD
                     LHLLVDRLVTKENDEELLQRVADSADAAFYEGHGELWLLEEGAEPMSFSNRFEADGIT
                     FEEPTPDFFNQNNPYGACRRCEGFGTIIGIDPSLVIPDPSLTVYQGAVACWRGEKMKA
                     WLERLVDHASLFDFPIHKPYNQLTDAQKKLLWTGNAHFQGLDDFFRMLEENAYKIQYR
                     VMLARYRGRTVCPECEGSRIRSDARYVLVAGKHIGELLDMPVDQLQAFIASMGLDVTE
                     EKIARRILTEVNGRLGYMLDLGLGYLTLNRRSNTLSGGETQRINLTRTLGSNLTSSLY
                     ILDEPSVGLHPRDTERLVRVLKELRNLGNTVVVVEHEEEVIRSADHLLDIGPEAGVHG
                     GKLVFSGTLQDLDGSKESLTAKYLTGKEVIEVPKQKRKPRNFIQIKQANMHNLKSLDV
                     RLPLHCLTAVTGVSGSGKTTLIRHLFAPELQRLLEHEADSPASSSLISGDWRKITQLE
                     MVTQDPIGKSSRSNPVTYVKAYDAIRELYASQGSAKARGYKPSHFSFNVDGGRCETCK
                     GEGEIVVDMQFLADVHLVCDDCGGKRFKEEILDIKYKDKNISDILAMSIEEAVAFFSG
                     EKDIAGKLQPLMDVGLGYVQLGQSSSTLSGGEAQRVKLASFLGKGRGRDHILFIFDEP
                     TTGLHFHDIRKLLDSFQMLIEHGHSVIVIEHNLEVIKCADWVCDLGPEGGEAGGYLVF
                     EGAPEELTNVAQSFTGKYIKEKF"
     gene            8497..8901
                     /locus_tag="PK742_04770"
     CDS             8497..8901
                     /locus_tag="PK742_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75937.1"
                     /translation="MKPTWRRLLAVLAGVITGGIVIFLMETIGHTVIPPPDHVDLSDI
                     DSMNAYMQEAPVAVLLFVVLGWLLGCLMGGWVAARIFKESLLASFICGTVLLVFSIIN
                     MTSFYHPVWVWIAALAGMVPAAWYGGRLGIRR"
     gene            complement(8891..9679)
                     /locus_tag="PK742_04775"
     CDS             complement(8891..9679)
                     /locus_tag="PK742_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQU75938.1"
                     /translation="MEKWLQVHGREWFYRDIGEGKPLLLLHGFCEFSMVWDPVLILLD
                     DRYRLIIPDLPGSGRSPVGASWTMHDLAADLLVLLDQEGLDEVVVMGHSMGGYLAMEM
                     LAGYPERLTGIGLIHSHVYADTEEKKESRRKAIRFVGENGARAYVRQLVPGLFAPLTD
                     RVLVRAEIDALDDQDPEGLQQALEAMINRLSTEDVLQNAHLPVLMVQGHHDTLLPMEN
                     LLAQAALAPQTELYVLPQAGHMGMLEQPREMAGHINDYLSRFIS"
     gene            9731..>12118
                     /locus_tag="PK742_04780"
     CDS             9731..>12118
                     /locus_tag="PK742_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQU75939.1"
                     /translation="MRYQFFLLLLCPLSLIAQVTFPVNGVHDDRHTPYAFTHMTLVQS
                     PTLTVEDATMIIQDGIIQSTGKGITIPEHAVVIDCSGLTLYPAFIDLACDAGIPEKPK
                     EKRTDGPQMESNDLRAVSWNQAVTPEFSAAGVFVPDTAANRKLRNAGFGAALTHREDG
                     IIRGTGAVVLLGDESANEMLLAEHSSLHYSFSKGSSSQDYPSSQMGSIALIRQSLYDA
                     QWYAAGGSEQETNLSLQALSDHLSHTAFFHVSDKLEILRASQIAKEFGLDLIAEGSGN
                     EYQRIDALKQSGAQLIIPLNFPDAPDVHDAWDAEQVALTTLQHWELAPANAAYLEKAG
                     MHIAITSDQLKDKKDFLPNLRKAVKYGLSEQEALSALTTTPAKMAGVSDQIGVLEKGK
                     QANFLVVAGNIFLDDGVLYEVWVHGVPYTVQQKPLSDQRGAWYLHLDNERYGLLIRGK
                     EQKPTFAVVRNTDTIDATGSLSGYNINLRFRLDSVSYNLSGTVRPGHFTGSGTRNGEW
                     MSWNAEKGNVYAEKETSVPVPDTLNLGAIPYPFQAFGNYTQPEPKSWLITNTTVWTNE
                     AGGILEQTDVLISNGKIIGIGKGLSAKDAEVIDGTGMHLTSGIIDEHSHIAISRGVNE
                     GTQSISAEVRIGDVINSEDVNIYRQLAGGVTTSHLLHGSANAIGGQTALIKLRWGDTP
                     EEMKFNGVDGFIKFALGENVKQSNWGDDNTIRYPQTRMGVEQIMEDGFTRAEAYIQAK
                     ANKNELVRTDLELEALAEILQSKRFITCHSYVQSEINMLMHLAERHGFKVNTFTHI"
BASE COUNT         3159 a         2911 c         3078 g         2970 t
ORIGIN      
        1 aaacctgacc gattatgaag taaaaggttc tgacatgtct gtatctctgc tgatgggact
       61 tggccggcaa tggacattca gagacatcgt cacactcgat ctttggtttg cactgggtta
      121 tggcggcaac tgggtgaatg ctgataccga caatctgccg gaagaaaatt attacaaccc
      181 tgatcagtcc ttcaaataca gctatgtgcg ttttggcgat tcgccgatga tctttgacgg
      241 cggactgagc gtggggattc ttttgaaata attcggaagg cgatggacgg tccctcccta
      301 ccgatggcta tcgggaacca tagggacgga attacttatg acgatccgct ttttacattc
      361 aactccctct ggtcagcggt gaaccttact ttcagcaagg agtgagaggt gaatggatct
      421 gtttcattac gattaacccc ttaacccttt ttcacctttt actcttatac aaacacaaat
      481 ccccgaagcg atcgcttcgg ggatttgtgt ttgtatttct gtatgtcaac gatcacatcg
      541 ccgcagcgaa gttggctgca accttgttcc agttgatgac attccagaac gctccgatat
      601 aatccgggcg acgattctgg tagttcaggt agtaggcgtg ttcccataca tccagtccga
      661 ggatgggtgt gccctttact tcagcaatgt ccatcagggt attgtcctgg ttgggcgtgc
      721 tggtcaccat cagtttcttt tcgccggtca cgatgagcca tgcccagccg gaaccgaaac
      781 gggtagcagc tgcattgctg aaagcttcct tgaatttgtc gaaaccaccc aggtcgcggt
      841 cgatggccgc agccaggtca cctgaagggg cgccaccggc attcggagcc atcacttccc
      901 agaacaggtt gtggttgaaa aatccgccgc cgttattgcg cacggcaccg gagatcttac
      961 ccacgttagc cagtatctct tcaatgctct tgccttccca ttcggtaccg gcgatagcgt
     1021 tgttcaggtt gttggtataa gctgcgtggt gtttgtcatg gtggatctcc atggtccggg
     1081 cgtcgatatg cggttccaga gcatcaaatg catacggaag cgcaggaagt gtaaatgcca
     1141 taatcagtat ttgatttaaa tgaggttatg aaattgagta cacctatata acgatatgta
     1201 aaggtaagag ttcatttgaa gaaagcagtg gggccccgta taacagctgc tgcatcgctt
     1261 tgctcacgca tctgtagcac gggatcagct ccttcgtcgc tgacagtgag aagtgaacac
     1321 tgcgtgagtg aggggtgagg gggagcagta tagcaatgaa gcagtatagc agtgagcagt
     1381 atatcagtaa gccgcatttg gggtaggatc gaaagtactc ctttactgct ggcgaagccg
     1441 actgcttatt gctatatggt attttaataa gaagagtcca attaactaat tgatccattc
     1501 cctcattcgc taattattac agaatacctt tgctccatgg gtatcctgac cgatccggat
     1561 tcgtatttca tgcagcaggc catccggttg gctgaacagg cactggaaca ggatgaagtg
     1621 cctgtaggtg cggtggtggt cagcaacgga cacatcatag cacgtgcaca caacctggtg
     1681 gaacgactgc atgatcctac agcgcatgcc gagatgcagg ccatcacggc agcctgcaat
     1741 catttcggca gcaagtacct gacagagtgc accctctatg tcactctgga gccctgcacc
     1801 atgtgtgcca ctgccatcta ctgggcgcag gtagggcgac tggtctatgg tgcttcagat
     1861 cccaaactgg gtttcaccag gcaccagcat atcatgcatc ctaaaacgca gatactgggt
     1921 gggattgaag aggacacctg cggacagctt ttgaaagatt tctttaaggg gaaaagatag
     1981 gatgagcccc gtataacagc tgctgtatcg ctttgcttac gcatctaaag cacgggatca
     2041 gctccttcgt cgctgacagt gagcagtgaa gggtgaaccc tgcgggagtg aggagtgagg
     2101 gtctctacac atgttttcca aaaacctttc gacccttgca cctttccacc ctttcaccct
     2161 ttcactcttt tccacccttg taccctttac cccgatagct atcgggggag cagtgaatgg
     2221 tgaaccttgc aggagtgagg agtgagagcc gctgaacatg tttgtctaaa aaaccttcga
     2281 cccttacacc cttttccacc cttcgacccc tgcacccttt ttcacccaaa aaaaagggaa
     2341 cagtttcctg ttccctttga tctgttcagt tacttgctga tcatttgatc accagtgtct
     2401 gtacatcgtt gcgctgacca tcggtcaggt gcacgaagta agtaccggca gccatatttt
     2461 cgagattgat ctcctgaacg gtgttatcgc cgtcaactgc aatgcttgca gtaaatacta
     2521 cctgacctac agtattggta atgttcacca tgacctgagc ggcatcatat ccactcagca
     2581 gcaggttgaa ctgaccgttg ttcgggttcg ggtagatggt catgctgttg ctgacatctc
     2641 cgatacgcaa tggcatggtg aagtcagttt gcggtccggg agctgagaaa cctgaaggat
     2701 agcaaacaga acgcagacgc actgtatagg agtggccggg ctccagtgct gtcagctcca
     2761 taaaggtatc tgtggtctga cgattccagc gttcgccggt ggtgttatca cgaatggcaa
     2821 acacatagcg ctctactcct tctaccggct cccaggtcac actggcgtag gtagatgaaa
     2881 tgatctctac agatggagca ccaggagtat tacataccgg aggagcagta cagctcaggt
     2941 aagaaatgat attggcacgc aggttagctg cttccactga agggctgtgg aagttattgg
     3001 tagtcatacc gccaaataac acagtaccag caccccaggc tttttcagcc agcacccagt
     3061 tgtctgtaga gaattcatcc cagataagcg ggttcagatc agtaccggta acaacagcat
     3121 gtccgaagga agtacccgtc cagttggtac caaccggagt aaaaggtccg gcaaaaatcg
     3181 ggtgaccggg atctgccgcc tcagcgttgt cagtaaaata tgcatacacc agagaggtgc
     3241 catcgaaacc aaagctcatg ccatcacctt cgttgggtgc agcattaaga agcagatgtc
     3301 cgccagaggc aacccatgct tcaatagcat cgatattggc atccaggaat gcttccagtt
     3361 catcggcgtg agaatcagaa ccttccagga acacaaagca attttcactg ctgaaggctg
     3421 cgtccacatc taccgtttca aagtattcat gggtataacc ttctccgaaa ttggcagcaa
     3481 gagcatccag gttagaactg gagaaccatg gttcaccgcc ggtggtgttg gagttgatat
     3541 aataagctcc ggtaccggta acaggcatca tatcggtaag cagcacatca tctatgtagt
     3601 agcgacaatc agcatctatg gaatagaagt caacagaacc gatattgccg gtataggcac
     3661 cggtatatac cattactcca tcgatccata tttcaatatt atcagcatcc aggtctatca
     3721 gatgttccac cttgaaccat tcgcccacgg gataggagaa ttcggcggcg ccgggaatca
     3781 gtacatttcc tgtaccgtca ggggctggtg taacgtaatc aaccagtgca aagaccagtt
     3841 ctgtgttcca ggctataccg ggtgtttcac tttcctggat gttgtagtag ccggacttac
     3901 cttccggtac cagcatttgc cattccaggc gccagtagcc acttgactgg tttccgagtt
     3961 tcaggatcac atcattcagg ccaccttctt caatggtcat ggaattgggt gctgaataag
     4021 cgtagaggtc ggtcacttcg ccatcttcag aaccaccttc tgcgccgctc caggtggtcc
     4081 accaggaggc gccgggcccg agggcatcgc ccgcggtata ggagtccatg ttatcacaaa
     4141 gtgttgcacc gggtatggca caaacggggt cctgagccac ggcgtttcct gcatagagga
     4201 ggcctgcggc catcagggca gacaggtaat gtatgcgttt cataacgatc aaattttcag
     4261 caatttagtt aagatgtgtt acgattgcaa acatgccgga aacaaaccgg ggctgcagcc
     4321 ggaagaaaac agtctgcaga aataaaaaaa ccccgccatg gcggggtgtc tcttacatca
     4381 tgtgttcggt aggtgcggtg ctcttgctgt atcgcaggat acgctttacc gtgtactcag
     4441 gtggtgaggc cattacggta tccaaacctc tggcagcatc acgtaattgt tcaaactgat
     4501 catgaaggtc tgtatctgta agcaaacttt ctctgatcag gccggtttct tcggggccgg
     4561 tttccccgta catgaagcga ataagatggt cttgtgtaag ggttttgatc atgcagtctg
     4621 tttaaggtga tgatttattt tatccaatac acaccttaaa cggacggtct tttctcccta
     4681 ttgtatcata tgctgatatt tttttcagcc atcattttcc ggatgttgat cagtgcatac
     4741 cgcatacgac ccagggccgt gttgatgctg acccctgtca tatctgctat ctccttgaaa
     4801 ctcataccgg aataatgacg caatatgagt acctgacgct gctcttcagg caggtggttc
     4861 acaaggttgc gcaccttatc ataggtattg tcatggatca gattctcttc aatgctcttg
     4921 tctttgaaac ccagtacatc gaaaatgtcg tagccatcgc cggtagtaac gatcggcatg
     4981 cgtttgtttc ggcggaaatg atcaatacac aggttatagg cgatgcgcat cacccagggc
     5041 agaaatttat cctgctcccg gtaacgacct gaacgcaggg tgtcaatgac cttcacaaag
     5101 gtgtcctgga agatgtcttc tgccaaagcc gcctccttca ccgtaaaaag gatggatgtg
     5161 tagatcttgt cgcggtgacg ggtgatcagt tctgatagtg catgctcatc acctgaaatg
     5221 tacaggcgca cgagttgctt gtcgggcatg gattgaactt gcatacgatt ataaggttgt
     5281 agtaaaaaac ttcagtaatt gttggcgtag aagtttgtcc ttataagcaa gttctgttgt
     5341 taggagttgt acttttgttg atccaaaaat aggattgttg cccaaattca ggtaacaata
     5401 cccccaaatg tacggttaaa atttattaag gtttcaaaag tttagttttt ttgccaaatt
     5461 tttgacaatt cataaacaat ttcagggatt ataatggctg tgggtaagaa aaagtctgaa
     5521 aaattagaaa ataaaaaaga gcagtactcc ggtatattca taaagggagc cagagagcat
     5581 aacctgcaga atattgacgt gaccattcca cgtaatcagc tggtggtggt gacaggtgtg
     5641 agcggcagcg gaaaatcttc gctgaccata gataccctct atgccgaagg ccagcgccgt
     5701 tatgtagaaa gcctgtcttc ttatgccaga caattcctga tgcgcctcaa caaacctgat
     5761 gtagattata tcaggggtat ctgcccggcc atcgcactgg accagaagac caccacccga
     5821 accacccgtt ctaccgtcgg caccctgaca gagatctatg actacctgcg cttgctgttc
     5881 gcccgggtgg gaaaaacctt ttcgccggtg agcggagaag aagttcgtaa gcattcggtc
     5941 actgatgtgg tggaccattt ctcctccatc gccgcaggca aaaaagtgtt catgaccgta
     6001 ccccttccaa gacagaaggg tcgttcgctc acggagaccg tgcatgtgct tcaccagaaa
     6061 ggatacaatc gtctgctggt tgatggcgat gtggtgaaga tggaagctgt gctggatggc
     6121 gaagcagaac tgaaagccaa acaggatctg catctgctgg tggacaggct ggttaccaaa
     6181 gagaacgatg aagaattgct gcaacgggtg gctgattctg ccgatgctgc attctatgaa
     6241 gggcacggcg aactctggct gctggaagaa ggtgctgaac ctatgtcttt ttccaatcgc
     6301 ttcgaggccg atggtatcac atttgaagaa cccaccccgg atttcttcaa ccagaacaat
     6361 ccctatggtg cctgcagacg ctgcgaaggt tttggtacca tcatcggcat tgatccatca
     6421 ctggtcattc ctgatccatc tcttactgtt taccaggggg ctgttgcctg ctggagggga
     6481 gagaaaatga aagcctggct ggaaagactg gtggaccatg catcactctt cgactttccc
     6541 atccacaaac cctacaacca actcacggat gcgcagaaga aattgctgtg gaccggcaac
     6601 gctcatttcc agggactgga tgattttttc cgcatgctgg aagagaatgc ctataagatc
     6661 cagtatcggg tcatgctggc cagataccgg ggccgcactg tttgtcccga atgcgaggga
     6721 agccgtatca gaagcgacgc acgctatgta ctggtagcgg gaaaacatat cggcgaactg
     6781 ctggatatgc cggtagatca gttgcaggct ttcattgctt ccatggggct cgatgtgacc
     6841 gaagaaaaga tcgcacgaag aattcttact gaagtgaacg gaagactggg gtatatgctc
     6901 gatctcggac tgggttacct gaccctgaac cgacgttcaa atacattgtc aggcggagag
     6961 acccaacgca tcaacctgac cagaacatta ggaagcaact taacttcctc cctgtatatc
     7021 ctggatgaac ccagcgttgg attacatccg cgtgatacag agcgacttgt tcgtgtattg
     7081 aaagaattgc gcaacctggg taacaccgtg gtggtggtgg aacacgagga agaagtgata
     7141 cgcagcgcag atcatctgct ggacattggt cccgaagcag gtgtacacgg aggaaaactg
     7201 gtattcagcg gaaccctgca ggacctggac ggatcgaagg aaagtctgac agcaaaatat
     7261 ctgactggaa aggaagtgat cgaggtgccg aagcagaaaa gaaaaccacg caatttcatc
     7321 cagatcaaac aggcgaatat gcataacctg aaatcgctgg atgtgcgact acccctgcat
     7381 tgcctgacag cggtgaccgg tgtgagtggc agcggcaaga ccaccctgat caggcatttg
     7441 ttcgcccccg aactgcagcg cttgctggaa cacgaagctg acagtccggc aagttcatca
     7501 ttgatcagtg gcgactggcg gaagatcacc cagctggaaa tggtgaccca ggatccgatc
     7561 ggaaaatcca gccggagcaa tcctgtaacc tatgtcaagg cctatgatgc cattcgcgaa
     7621 ctgtatgcat ctcagggatc agctaaagcc aggggataca agcccagtca tttttccttc
     7681 aatgtggatg gaggacgatg tgaaacctgt aaaggtgaag gagagatcgt ggtggacatg
     7741 cagttcctgg cagatgtgca tctggtatgc gatgactgtg gcgggaaaag attcaaagaa
     7801 gagatacttg atattaagta taaagataaa aacatatctg atatacttgc catgagtata
     7861 gaggaagcag tagctttctt ttcaggcgaa aaagatattg caggcaaact gcagcctctt
     7921 atggatgtag gtctgggtta tgtacagctg ggacaaagca gctccaccct gagcggcggt
     7981 gaggcccagc gggtcaaact ggcaagcttt ctggggaaag gacgcggaag ggatcacata
     8041 cttttcatat ttgacgaacc aaccaccggt ctgcattttc acgacatacg caaactgctg
     8101 gattctttcc agatgctcat cgaacatggt cacagcgtca tcgtgataga acacaacctg
     8161 gaggtcatca agtgtgctga ctgggtgtgc gatctcgggc cggaaggcgg tgaagccggt
     8221 ggttatctgg tctttgaagg agcaccggaa gaattgacca atgtggcaca aagtttcacc
     8281 ggaaaatata taaaggagaa gttttgagga gcagggaagc cgtatagctg tgaaggagtg
     8341 ggcagtgaat ggtgaggatg gagggacgtt atccaggttg aacacagaat ccgatacacc
     8401 gacagctccc gatagttaca gggaggggtt ccatccttat acccttatcc agtcttcctc
     8461 accctttgac cttttctatt ttcttatttt agttccatga aaccaacctg gcgccgactc
     8521 cttgccgtac tggccggtgt gataaccgga ggcattgtga tctttctgat ggaaaccatc
     8581 gggcataccg tcataccacc accagatcat gtggatctgt cagatattga ttccatgaat
     8641 gcctatatgc aggaagcacc tgtggcagtt ttgctttttg tggtgctggg ctggctgtta
     8701 ggctgtctga tgggtgggtg ggttgctgcc aggatattca aagaatctct gctggcatca
     8761 ttcatttgtg gtacggttct tctggtcttc agcattatca atatgaccag cttctatcat
     8821 ccggtatggg tttggatagc agctcttgcg gggatggtac cggcagcctg gtacggaggc
     8881 agacttggca tcaggagata aacctgctca gataatcatt tatatgtcct gccatttctc
     8941 ttggctgttc cagcatgccc atatggccag cttgcggaag tacatacagt tcagtttgcg
     9001 gtgccagtgc agcctgagcg agaaggtttt ccatcggtaa cagcgtatca tgatgacctt
     9061 gcaccatcag gacaggcaga tgcgcatttt gcagcacatc ttctgttgac agccggttta
     9121 tcatagcttc caaagcctgc tgaagcccct ccggatcctg atcatccagt gcatctattt
     9181 ctgcccgaac cagtacacgg tcagtaagcg gtgcaaacaa tcccggaaca agctggcgca
     9241 cataggccct tgctccgttt tcacctacaa aacggatcgc ctttcttctg ctttcttttt
     9301 tctcttcagt gtcagcataa acatgagagt ggatcagacc aatacctgta agtctttccg
     9361 gatatcctgc aagcatttcc atggccagat agcctcccat ggaatgcccc atgaccacca
     9421 cctcatccag gccttcctgg tcaagaagta caagaagatc tgctgccaga tcatgcattg
     9481 tccaggaagc cccgaccggt gaacgaccgg aaccgggcag gtcaggtatg atcagacggt
     9541 acctgtcgtc gagcagaatg agcaccggat cccagaccat actgaactca cagaacccat
     9601 gcagtaacag caagggtttc ccctctccga tatcgcggta gaaccattcc ctgccatgca
     9661 cctgcagcca tttttccatg atggcatgca agatataaaa tccctatttt cgtcagccaa
     9721 gcatttactc atgcgttacc aatttttcct actcctgtta tgcccgttat cattgatcgc
     9781 acaggtaact tttcctgtaa atggtgtaca tgatgaccgg catacaccct atgcatttac
     9841 ccatatgacc ctggtgcaat cgcccactct tacagtggaa gatgccacca tgatcattca
     9901 ggacggcatc attcagtcaa ccggcaaggg aataaccatt ccagaacatg ctgtggtaat
     9961 agattgcagt ggattgacct tgtatccggc cttcattgac ctggcgtgtg atgccggcat
    10021 accggagaaa ccaaaagaga agagaactga cggccctcag atggaaagca atgacctgcg
    10081 ggcagtcagc tggaatcagg cagttacgcc tgaattttct gcagccggtg ttttcgtacc
    10141 agacactgcc gccaacagaa aacttcgcaa tgccggtttt ggtgctgccc tgacccaccg
    10201 ggaagatggc atcatacggg gaaccggcgc agtggtcttg ctgggcgatg aatctgccaa
    10261 tgaaatgcta ctggctgagc atagcagttt gcactattca ttcagcaaag gaagttcttc
    10321 acaggattat ccaagctcac agatgggcag tattgcgctg atcaggcaga gcctgtatga
    10381 tgcacagtgg tatgcagccg ggggttctga acaggaaacc aacctttctt tacaggcgtt
    10441 gtctgatcat ttatcgcata ctgctttttt tcatgtttct gacaaactgg agatccttcg
    10501 ggcttcacaa atagctaaag aatttgggtt ggatctaatt gcagaaggaa gcggtaatga
    10561 ataccagcgc atcgatgcgt tgaaacaatc aggagcgcaa ctcatcattc ccctgaactt
    10621 tccggatgca cctgatgtac acgatgcctg ggatgcagag caggtagcat tgaccacact
    10681 gcagcactgg gagctggctc ctgccaatgc agcctatctg gaaaaggcgg gaatgcatat
    10741 agccatcaca tcagatcagc tcaaagacaa aaaagatttt ctgccgaatc ttcgtaaggc
    10801 tgttaaatat ggcctgagtg aacaggaggc tctgtctgcc ctcactacca caccggcaaa
    10861 gatggcagga gtaagtgacc agatcggcgt actggaaaaa ggcaagcaag ccaacttcct
    10921 ggtagtagca ggaaatatat tcctggacga tggtgtgttg tatgaagttt gggtgcatgg
    10981 agtaccttat acagtacagc aaaagccatt gagcgatcag cggggagcat ggtacctgca
    11041 cctcgataat gagcgatacg gactactgat cagaggaaag gaacaaaaac ccacatttgc
    11101 agttgttcgg aacactgata ccattgatgc taccggcagc ctgagtggat acaatattaa
    11161 tcttcgtttc aggctggaca gtgtcagcta taatctgagt ggaaccgtca gacccggaca
    11221 cttcaccgga agtggtaccc gcaatggaga gtggatgagc tggaatgctg aaaaaggtaa
    11281 tgtgtatgca gaaaaagaaa catctgttcc cgtacctgac accctgaacc tgggagccat
    11341 tccttatccc ttccaggcat ttggcaatta tacacaaccg gaaccaaaat cctggctgat
    11401 caccaataca acagtgtgga ccaatgaagc cgggggcatt cttgaacaaa ccgatgtact
    11461 catcagcaac gggaagatca tcggtatagg aaaagggctg agtgccaaag atgcagaggt
    11521 cattgatggc accggcatgc accttaccag cggcatcata gatgaacatt cgcatattgc
    11581 catcagcaga ggtgtgaatg aaggtaccca gtctatttct gccgaagtgc gcattggaga
    11641 tgtcatcaat tcagaagatg ttaatattta ccggcagctg gcaggaggtg ttaccacttc
    11701 tcacctcctg cacggctctg caaatgccat aggtggtcag accgcactga tcaaattgcg
    11761 ctggggtgat acacctgaag aaatgaagtt caacggtgta gatggtttca tcaaattcgc
    11821 tcttggagag aacgtcaaac aatccaactg gggcgatgac aatacgatca gatatccgca
    11881 aacgcgtatg ggtgtggaac agatcatgga agacggattc acccgggcag aagcatacat
    11941 ccaggcaaaa gccaacaaga atgaattggt tcgcaccgat ctggaactcg aagcactggc
    12001 cgagatactc cagtcaaaaa gattcattac ctgccacagc tatgtgcaga gcgagatcaa
    12061 catgctgatg catctggcag agcgccacgg attcaaggtg aacaccttca cccacatt
//
LOCUS       DAOMAX010000058        11956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_685_length_11956_cov_4.143181, whole genome shotgun sequence.
ACCESSION   DAOMAX010000058 DAOMAX010000000
VERSION     DAOMAX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_11956_cov_4.143181"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK742_04785"
     CDS             complement(<1..598)
                     /locus_tag="PK742_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HQU75940.1"
                     /translation="MRFYFLITSLLAFCSTLSAQNTNLHLLITDKYGLPVEAAFIDDL
                     LYQVNGITNSEGKADLYAAPGSYLLFITSIGYYDTAVTVTSPSTVHIQLRKKIYDIPV
                     VYVYGNTAGVHHPLTYTNISKKELQSLNYGQDMPMILEQTPSAVATSDAGNGVGYTGI
                     RIRGSDPTRVNISLNGIPFNDAESQQAYWVRIRCRSARS"
     regulatory      complement(639..735)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(759..1268)
                     /locus_tag="PK742_04790"
     CDS             complement(759..1268)
                     /locus_tag="PK742_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU75941.1"
                     /translation="MIRHVVITGPESSGKSTLATLLGEQLHWPVVDEVARTLLTASGP
                     VYSREDVIRIGLTQLQYNQQMLMQKGRVVADTDLLTIHIWLEDKFGAADESLIQLWLD
                     HIPDLYLLQTPDMPWQPDPLREDPERRTYLFYRYRSWLDQYACPYLVCDNTVNERLIR
                     SLEAIRQDR"
     gene            complement(1265..1939)
                     /gene="pnuC"
                     /locus_tag="PK742_04795"
     CDS             complement(1265..1939)
                     /gene="pnuC"
                     /locus_tag="PK742_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HQU75942.1"
                     /translation="MFLNVWQDIAAMPAVEVVAAACGVLSVWFARGNSIWVYPTGIVS
                     VTLYILICLQVGLYADSLINAYYLFMSIYGWYFWLHGGKIQAAESHRSSEALDDFTEE
                     TGTAEEKAHISYNTLGMNLRWLGVTALVYVLMAMLLSAYTDSDVAWIDAFTTAVFLTA
                     MYAMARKKIEHWIFWILGDAVSIPLYIYKGLPVTSVQYVVFLALAIWGFAVWYRDLEK
                     QMKLSE"
     gene            complement(1964..2668)
                     /locus_tag="PK742_04800"
     CDS             complement(1964..2668)
                     /locus_tag="PK742_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HQU75943.1"
                     /translation="MRIQQLMAIATLTTGSMFVAGCDLFSLDDLNSGLTEAEVVEGLK
                     EALNVGTDTAVAQGSSLNGFFLNPEIKIPFPEEVSIVKTVVESVPGGSLLVDEFVTQL
                     NRAAEDAAEKATPIFKDAILNITFTDAFNILNGADTAATSYLRTNTFSALYDAFKPDI
                     ETSLTNVGAQGAWEAVVNVYNAVPFTDPVSADLADYTTNKGLKGLFVLVGNEEVKIRN
                     DISHQVSDILQKVFGN"
     gene            2882..3550
                     /locus_tag="PK742_04805"
     CDS             2882..3550
                     /locus_tag="PK742_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75944.1"
                     /translation="MIIDDTGGGTLPIDTGGTGGIDTTFYTGIPCDPDTVYFQNDILP
                     ILVSNCAIEGCHDVASHEEGIILSTYDYVMSTGTVHAGHPNSSEMYEKITDFGDDDLM
                     PPPPAPPLSSDEIALIYTWIDQGALDNYCSDCDTSSVTFSETVWPIVDAYCTGCHDAT
                     LPSAGLSLLNYDDVAFVAENGSLMSSLNGSDGYVLMPSGSSALPDCLIDLIQTWVDDG
                     YPDN"
     gene            3566..4246
                     /locus_tag="PK742_04810"
     CDS             3566..4246
                     /locus_tag="PK742_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75945.1"
                     /translation="MRRLQWLAVILVIVSWFAESCQHTPFNFGELLISENCSPDTAYF
                     VNEAFPIINSNCAMSGCHDAITHEEGVNLSSWDAIMNSDVVKPGNANGSKLIEVLTES
                     GEEAMPPDGPLSSEQIDVLKLWINQGALNNECLDGNCDTTSVHFGSTIDPLINIYCEG
                     CHSGSSPSGGINLTTYSNIADFAASGQLLGAVEHSAGYEPMPPGSSLTNCKTDQIRIW
                     IENGYPND"
     gene            4239..4628
                     /locus_tag="PK742_04815"
     CDS             4239..4628
                     /locus_tag="PK742_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75946.1"
                     /translation="MIKKVHLFALSASGLILAASLSSCYYDNVETLTAGNVCDTSAVS
                     YANDIVPVLQGNCYVCHGTGSYTSSGAGILVEGYEATADLANTGFLTAVIDWTDGASP
                     MPQGSPQIPACERAKIRNWVSQGAQNN"
     gene            4630..5508
                     /locus_tag="PK742_04820"
     CDS             4630..5508
                     /locus_tag="PK742_04820"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HQU75947.1"
                     /translation="MKNLITTILLGCAVSVQAQTSTAHDIFSDTRIITSQSVETNWKG
                     EGTFIISHRFGDIYQNDAYSILYNFLGFDGGANMRIGLDYGITDRLMIGGGRSGYNKT
                     YDAFAKWNIMRQQSGDKNLPVAITLYSDISFISDTTNAVLDSFFVDRFSYAYQLMIAR
                     KFNEKLSVQIMPTLIHRNLVATKAESNDVFALGAAVKYNLTPVLAISAEYFYNLPGQL
                     PAGFNDYSALGIDIKTKGHVFQIQLTNSPFLIPEYYIGATQGNIIDQDASGQFDLNLR
                     LGFNITRDFQLGGRQY"
     gene            complement(5511..5966)
                     /locus_tag="PK742_04825"
     CDS             complement(5511..5966)
                     /locus_tag="PK742_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75948.1"
                     /translation="MITAVTLAYIGIGLMIALSGVGSAIGVSIGGKATIGALKKKPEA
                     FGNYMLLSALPGSQGLYGFAGFFIVSGKLQGLTDMTIFQGASVLAAGMALGLVGLFSA
                     IQQGQVTAHGIEGIASGHDVFGKTMVLAVFPELYAIVAFAATFLISASL"
     gene            complement(5963..7753)
                     /locus_tag="PK742_04830"
     CDS             complement(5963..7753)
                     /locus_tag="PK742_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQU75949.1"
                     /translation="MIAPMLKYQLLVHHRELSQVLNELRKLGIAHIDTTWNDQDAAAK
                     EDQLRNYRRLQERYQWLSNFKAADLPENPPLNTEALIIETDQIRTRLSELEKETRETR
                     LRWKVLEPWGDIPAGRLQSLQEYGIYTYFFHCPAGSFQQDWKKEMALEIIHEDKHQVH
                     FVVLSDRLIPPSLNASMLDLKDASLQELAIAQHQQQQEHRALRERLMTLAACIPMLLA
                     EMLRLEEESEWENVLNGTQVLADGKLHLIHCWVPENRSELFVSFLNHSNLVWKVSRPT
                     PDESTPVLLQNDRFSRLFHPVGELFSLPAYTELDLTPFFAPFFTLFFGMCLGDVGYGL
                     IILLALTVLKWSRRAPALRNILTLGQFLGTSAAVFGLLTGTVFGADLSKHSAEWLVQW
                     QTYFLSVDQLFNLSLSVGLIQILFGMALRVWNRSRLFGWLYGLSSLGWILAITGGLLS
                     VGWNRQLSGMVLVIAGLALIIFFSDPGKGLLSRFGLGLWDLYGVTGLFGDVLSYVRLF
                     ALGLSSGILGFVVNNIAFSLLDGRSVIGILFFVIILLFGHGLNFAISTLSAFVHPIRL
                     TFVEFYKNAGFTGGGIQYNPFMKKHFKTES"
     gene            complement(7750..8343)
                     /locus_tag="PK742_04835"
     CDS             complement(7750..8343)
                     /locus_tag="PK742_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQU75950.1"
                     /translation="MSLQIAYNKIALQGLEKALKIRESALSTIRSKESALRTEVKKQL
                     LVLKEVHQALLDHQQEGERFAGLWSEWDTSLVSVHHIQTRTGSFAGVRYRIFERADIS
                     ERPFDFRSNPPWWPQGVSYVKQAYTLQIRYKLQKEFLDKLEKERKKTTQKLNLYEKVQ
                     IPECREGIRKIKRFLEDQETLEKATQKIVKKKKSNLS"
     gene            complement(8343..9662)
                     /locus_tag="PK742_04840"
     CDS             complement(8343..9662)
                     /locus_tag="PK742_04840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQU75951.1"
                     /translation="MQTSVINRFYTDIHHITKATISLRATGIGNEELALVDGRLAQVV
                     KMEGDMVTLQVFSGTEGIANDAKVSFLGTAPALEVDERLRGRFFNAFGKPLDEWPAPT
                     GRKREIGGPPVNPVRRKQPNTPIFTGIAGIDLNNTILGGQKIPFFADPDQPYNEVMAT
                     VALRAKADKIILGGMGLSNDDLLFFKDTFRRAGVLDKIIAFVNTTEDPPVERLLVPDM
                     VLTAAEFFAVDKDEQVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLARIY
                     EKAVQFEGKGSITIIAVTTLNQGDITHAIPDNTGYITEGQLFLRKDSETGKVIIDPFR
                     SLSRLKQLVIGKQTREDHPQIMNACIRLYADAAEARIKKENGFELTDYDIRTLAYARD
                     YTAELLSIDVQMDMDEMLNKAWQLISKHFQPDETGIRQELVDRYWMN"
     gene            complement(9664..11427)
                     /locus_tag="PK742_04845"
     CDS             complement(9664..11427)
                     /locus_tag="PK742_04845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQU75952.1"
                     /translation="MKVRGTVQGLIANLVQVKPDGPVRQNEICYLQAGEDLLMGEIIK
                     ISGEIAFVQVYDSTRGVRIGAEVNFTGDLLEVTLGPGMLGKKYDGLQSDLDAHEGTFL
                     LRGMRSPALDVHQKWDFRPLVQTGSMVSAGAWLGEITENGLPHKIMAPFSMEGQGKIA
                     EIKPAGEYKAADTVAEIIMTDGRKHAVTMVSTWPSKLALKAYGRKIRPFKMLETGIRT
                     IDTVHPMLEGGTGFIPGPFGCGKTVLQQAIAKQAKADIVVIAACGERANEVVEIFTEF
                     PELDDPATGRKLIERTIIIANTSSMPVAAREASVYTAMTICEYYRNMGLRVLLLADST
                     SRWAQALREMSNRMEDLPGPDAFPMDLTATIAAFYARAGYVELTNGKQGSITFIGTVS
                     PAGGNLKEPVTEATKKAARCFYALSQRRADSKRYPAVDPIDSYSKYLEYPEFRDYMKE
                     HVSTRWIAHANEIKNILLRGKEAADQINILGDDGVSIDYHMQFWKSELLDFILLQQDA
                     FDEVDMFCSVTRQEFMADLLLQICQTEYHFDHFSEVRPFFRKMINSLKQLNYAIFESP
                     GFNRILEEVRSMAIQPAEKSI"
     gene            complement(11424..>11956)
                     /locus_tag="PK742_04850"
     CDS             complement(11424..>11956)
                     /locus_tag="PK742_04850"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HQU75953.1"
                     /translation="LQQNLKLFPYQDQMLFPGKESPRISLEASWRIAWTTFVRQTNNR
                     LLHEYTVLQEALFLIRILLVEDNGDAIADVHRLSGVEEPNISWPDYHRRILEATGISG
                     QIMRLMTIRHPEELEWEADNLAWNWLEERLFQKEFRIDRLLAYAWQQQLIARWSRIRN
                     ADAKVPVFDIDVNTYV"
BASE COUNT         3109 a         2868 c         2675 g         3304 t
ORIGIN      
        1 tcgacctggc cgatctgcac ctgatgcgta cccagtaggc ctgttgcgac tctgcgtcat
       61 tgaaagggat accattcagg ctgatgttga ccctggtagg gtcagagccc cgaatgcgga
      121 tccctgtgta ccctaccccg tttccggcgt ctgaagttgc aactgcagat ggggtttgtt
      181 ccagtatcat aggcatatcc tggccgtagt tcagggactg aagttccttt ttactgatat
      241 tggtataggt tagcggatgg tgaacaccgg ctgtattacc atatacatac acaacaggta
      301 tatcatatat ttttttacgc agctgtatat gcacggtgga tggcgatgtt acagttacag
      361 ctgtatcata gtagccaatg gaagtgatga acagcagata acttcccggg gcggcataca
      421 ggtcagcttt accctctgag ttggtgatgc catttacctg gtatagcaga tcatcaatga
      481 atgctgcttc cactggtagt ccgtacttat ctgtgatgag cagatggagg ttcgtgttct
      541 gagctgacag ggtgctacag aaggcaagaa gcgatgtgat caaaaaataa aagcgcatac
      601 ggttagtttg cgcctgtcaa atggggcctt agccagacaa tgtatttttt cctctttccc
      661 ttccgggtat tgccccggca ggttcgatgg gtatgatctc agatgcaccc aggtgcaaca
      721 ccccatgaga tggggcaaag gtaagcaaat ctgactgttt accggtcttg tctgatcgcc
      781 tccagactcc tgatcagtct ttcatttacc gtattatcac ataccaggta agggcaggcg
      841 tattggtcca gccaggatct gtacctgtaa aacaggtaag ttcttctttc cggatcttcc
      901 cgcagcggat ccggttgcca gggcatatca ggcgtttgca acaaatagag atcaggaatg
      961 tgatccagcc acaattgaat gagagactca tccgcagcac cgaatttatc ttccagccag
     1021 atgtgaatgg tcagcagatc agtatcagcc actacccttc ccttctgcat cagcatctgt
     1081 tgattgtact gaagttgagt cagtccgata cggataacat cttcgcggct ataaacaggt
     1141 ccggaagccg tcagcaatgt tctggccact tcatccacta ccggccagtg cagctgttca
     1201 cccaggaggg tggccaaagt ggactttccg gatgattccg ggccggttat taccacgtgc
     1261 ctgatcattc agacagcttc atttgttttt ccaggtcgcg gtaccaaact gcaaagcccc
     1321 agatggccag agccagaaag accacatatt ggacagaggt aaccggtaat cctttgtaga
     1381 tataaagagg gatactgact gcatcgccaa ggatccagaa tatccagtgt tcaatttttt
     1441 ttctggccat ggcatacatg gcagtcagga aaactgctgt ggtgaaagca tcgatccagg
     1501 ctacgtctga atctgtatag gcagacagca gcatcgccat aagtacatac accagtgcag
     1561 tgactcccag ccaccgaaga ttcatcccaa gagtgttata agagatatgg gccttttctt
     1621 ctgctgtacc cgtttcttca gtaaaatcat ccaacgcttc agaactgcgg tgcgattcag
     1681 ccgcctgaat ttttccgcca tgcagccaga aataccaacc atatatactc atgaagagat
     1741 aatatgcatt gataagcgag tcagcataca gtccgacctg taggcagatc agtatatata
     1801 gtgtaacact gacaatacct gtaggataga cccagatgct gttaccgcgg gcaaaccaga
     1861 cactcagcac accacaggca gcagcgacca cttccacagc cggcatagct gcaatatcct
     1921 gccagacatt caggaacatg cggtaaaaat gcccagtggt atatcagttt ccaaatactt
     1981 tttgcaaaat atcagaaacc tgatggctga tgtcgttgcg aattttgacc tcttcatttc
     2041 ccaccaggac aaacaagcct ttcagtcctt tattagtggt atagtctgcc aggtcagcac
     2101 tgaccgggtc ggtaaaggga acggcattat atacattcac gactgcttcc caggctccct
     2161 gtgcacccac atttgtcagt gatgtttcaa tgtcgggctt gaatgcatca tataaagcac
     2221 taaaggtatt ggtgcgaagg tagctggtag cggcagtgtc tgctccattc agaatattga
     2281 aggcgtcagt aaaggtgata ttcagaatag catctttaaa aatgggagta gctttttctg
     2341 cagcatcttc ggcagcccgg ttgagctgag ttacgaattc gtccaccagc agtgaaccgc
     2401 cgggaacaga ctccactact gttttaacga tgctcacctc ttcgggaaac gggatcttga
     2461 tctccggatt gaggaaaaat ccattgagcg aactaccctg agcaacagct gtatctgtac
     2521 ctacattcag tgcctctttc aaaccttcta ccacctcggc ctcggtcagg ccggaattga
     2581 ggtcgtccag tgaaaagaga tcgcagcctg ccacaaacat tgagccggtt gtcagggtgg
     2641 caattgccat tagttgctga atacgcattt gattatcctt ttctgagtct gacgaaatat
     2701 tatgccaaaa ttgagcgttt ttgctttaag tcaccggaaa acgagaaaaa atgtccccat
     2761 tttcagtttg gtgaatcttt aactttgcat taaagttagc atatcaagta tgaaaaggtc
     2821 cttatttctc ctctctggcg gcctcttttt tttggcggca tgtgttcatg atcctttcgc
     2881 agtgattatt gacgataccg gtggcggaac gctacccatc gatacgggtg gaacaggtgg
     2941 tatagacacc acattctaca caggtattcc ctgcgaccct gatacggtct atttccagaa
     3001 tgatatcctt cctattcttg taagcaactg tgccatcgaa gggtgtcatg atgtggcctc
     3061 ccatgaggaa ggtatcatac tcagtaccta tgactatgtg atgtcaaccg gaacggtgca
     3121 tgcgggtcac ccgaattcca gcgagatgta tgagaagatc actgattttg gtgatgatga
     3181 cctgatgcca ccaccacctg caccgccgct gtcttcagat gaaattgccc tgatctatac
     3241 ctggatcgat cagggagcgc tggataatta ttgttctgat tgtgatacca gttcagttac
     3301 tttcagcgaa acagtttggc ctatagttga tgcttattgt acaggttgcc atgacgcaac
     3361 gctgccctct gccggactta gtttgctgaa ctatgatgac gtggcgtttg ttgctgagaa
     3421 cggctcgctg atgagctcac tgaatggttc agatggatat gtcctgatgc caagcggaag
     3481 ttcagcttta cccgattgtt tgatcgatct gatccagaca tgggttgatg atggatatcc
     3541 ggataattaa catttaagga tcagcatgag aagattacag tggcttgcag tgattctggt
     3601 catagtttct tggtttgcag aatcttgtca gcatacccca ttcaacttcg gagagcttct
     3661 gatctctgag aactgttcac cggatactgc ctattttgta aacgaagcgt tcccgatcat
     3721 caacagcaac tgcgccatga gtggttgcca tgatgccatt acacatgaag aaggtgtaaa
     3781 tctcagttcc tgggatgcca tcatgaacag cgatgtggtc aaaccgggga acgctaatgg
     3841 cagtaaactg attgaagtgc tgacagaaag tggagaagaa gctatgcccc ctgatggccc
     3901 gctcagttca gaacagatcg atgtgctgaa gttgtggatc aaccagggag cattgaacaa
     3961 tgagtgcctg gatggtaatt gtgataccac ctctgtgcat ttcggatcca ctattgatcc
     4021 tctcattaat atttactgtg aaggttgtca ttcaggaagt tcaccatccg gtggtattaa
     4081 tctgacaaca tatagtaata ttgcagactt tgcagcaagc gggcaactat tgggcgcagt
     4141 agaacacagt gccggatacg aaccaatgcc acccggcagc tcactgacaa attgtaaaac
     4201 agaccagatc aggatctgga tagaaaacgg ttatcctaat gattaagaaa gttcaccttt
     4261 ttgcattgtc tgcctcaggt ttgatacttg cagcttcttt gtcttcctgt tattacgata
     4321 atgtggagac actgacagca ggcaatgttt gtgatacttc ggctgttagt tatgcgaatg
     4381 acattgttcc tgttttgcag ggcaattgtt atgtttgcca tggtaccggc agttatacca
     4441 gcagtggtgc aggcatattg gttgaaggat atgaagcaac tgcagatctg gcaaacaccg
     4501 gttttctgac tgccgttatt gactggaccg atggagcgtc tcctatgcct cagggatcgc
     4561 cgcagatacc tgcatgtgaa agggccaaga tcagaaactg ggttagtcag ggagcacaaa
     4621 acaactaaca tgaaaaacct gatcactacc attttgctgg gctgtgctgt atctgtacag
     4681 gctcaaacaa gtacggcgca tgatattttt tcagataccc ggatcatcac ttcgcaatca
     4741 gtagagacca actggaaagg tgaaggtact tttatcatat ctcaccggtt tggagatata
     4801 taccagaacg atgcctattc catattatat aatttcttag gttttgatgg cggtgccaat
     4861 atgcgcatcg ggctggacta tggcattact gaccggctga tgattggcgg tggcaggagt
     4921 ggttacaaca agacctacga tgcatttgcc aaatggaaca tcatgcgtca gcagtcagga
     4981 gataaaaacc ttcctgtagc tataacttta tacagtgata tatcctttat atcagatacg
     5041 accaatgcag ttcttgacag tttttttgtg gacagattca gctatgccta tcagttgatg
     5101 atagccagaa agttcaatga gaagctgagt gtacaaatca tgcctacttt gatacaccgg
     5161 aatctggtgg caaccaaagc agaaagtaat gatgtttttg cccttggtgc tgcagtgaag
     5221 tacaacctga caccggtgct tgcaatatct gcagaatact tttacaatct gccaggacag
     5281 ttaccggccg ggttcaatga ttattcagcg cttggtatag atatcaagac caaggggcat
     5341 gtctttcaga ttcagctgac caattctccg ttccttattc cggaatacta cataggtgct
     5401 acccagggta acatcattga ccaggatgcc agtggtcagt tcgatctgaa tctgcgtctg
     5461 ggtttcaata ttacccgtga cttccagctt ggaggacgac aatactgagc ttacagagag
     5521 gcgctgatca ggaaggttgc tgcaaaggca acgatagcgt agagttcagg aaaaactgcc
     5581 aataccattg tttttccaaa tacatcgtgc cctgatgcaa taccttcgat accatgtgcc
     5641 gttacctgac cttgttgaat tgctgaaaac aatccaacca gaccaagagc cataccggca
     5701 gcgagtactg aagctccctg aaatatggtc atatcagtca gcccctgcag ttttccgctt
     5761 acaataaaaa atcctgcaaa accatacaag ccctgtgatc cgggtaaggc actcagcaac
     5821 atgtaattac caaatgcctc aggcttcttt ttgagggctc cgatggtagc ttttcctcca
     5881 atggacacgc caattgcgct accaacccca gacaaggcga tcattaatcc aattcctatg
     5941 taggctaatg ttactgcagt gatcatgatt ctgttttaaa gtgttttttc ataaatggat
     6001 tatactgtat gccgcctccg gtaaagccgg cattcttata aaattcaaca aatgtgagtc
     6061 ttatcgggtg tacaaaagca ctgagggttg aaattgcaaa attcaaaccg tgaccaaaaa
     6121 gcaggatgat cacaaaaaac aagatgccaa tgacagatct gccgtccagc aaactaaagg
     6181 ctatgttgtt caccacaaaa ccaaggatac cggatgacaa gcccaatgca aacaagcgga
     6241 cataagacaa cacatcacca aaaagccctg ttacgccata caggtcccac aggcccaggc
     6301 caaatctgga gagcagtcct ttaccgggat cgctgaaaaa gatgatcaga gccagtccag
     6361 ctatgaccag caccatacct gatagctgcc ggttccatcc tacactaagc agtccacctg
     6421 taatggccag tatccatccc agagaagaca agccatatag ccagccaaac agccgacttc
     6481 ggttccatac ccggagagcc attccaaaca ggatctgtat caggccaaca gacaacgaca
     6541 ggttgaacaa ctgatcaaca gaaagaaaat atgtctgcca ttgcaccaac cattctgcgc
     6601 tatgcttact aagatcagcg ccaaagacag tgccggtaag caggccaaag acagctgcag
     6661 aagttccaag aaactgtccc agtgtcagaa tatttctaag cgcaggtgct ctcctactcc
     6721 atttgagcac ggtaagggcc agcaggataa tgaggccgta tcccacatcg cccagacaca
     6781 taccaaaaaa aagggtgaag aaaggtgcaa aaaacggggt caggtcgagt tctgtatatg
     6841 cgggcaaact gaacagctct cctacaggat ggaataacct gctgaaccgg tcattctgta
     6901 acaaaaccgg agtactttca tcgggagttg gccgggacac cttccatacc agattgctgt
     6961 gattcaaaaa cgaaacaaac aattctgatc gattctcggg cacccagcaa tggatcagat
     7021 gtagtttgcc atcagccagc acctgtgtgc cattcaggac attctcccac tctgattctt
     7081 cttccagccg aagcatctct gccagtaaca ttgggatgca ggccgcaagt gtcatcaggc
     7141 gttcccggag agccctgtgc tcttgttgtt gttgatgttg tgcaatagca agttcctgca
     7201 gagaagcatc tttcagatca agcatggatg cattcaggga aggtggaatc aaacgatctg
     7261 aaagcaccac aaaatgtacc tgatgcttgt cttcatgaat gatctccagg gccatctctt
     7321 ttttccagtc ttgctgaaat gagcccgcag ggcaatgaaa gaaataggta taaattccat
     7381 attcttgaag cgattgtaac cgacctgcag gaatatcgcc ccatggttcc agtactttcc
     7441 atcgcaatct agtttcacgg gtctcttttt ccaattcaga aagcctggtt ctgatctgat
     7501 cagtttcgat gatcaatgct tcagtattta acggaggatt ctccggcagg tctgcagctt
     7561 taaagttgct cagccactga tacctttcct gcagccggcg ataatttctc agttgatctt
     7621 cctttgctgc tgcgtcctga tcattccagg tagtatcgat atgcgcaatg cccagttttc
     7681 tgagctcatt cagcacctgc gaaagttcac gatgatgcac cagcagttga tatttcagca
     7741 taggtgcgat catgataaat tgcttttttt ctttttcacg atcttctggg ttgctttctc
     7801 cagtgtttcc tgatcttcca gaaaacgttt gatctttctg atgccttctc tgcattcagg
     7861 tatctgaacc ttttcataca gattgagctt ctgtgtggtc ttctttctct ccttttccag
     7921 tttatccaga aattctttct ggagcttata tctgatttgc agggtataag cttgctttac
     7981 atagcttaca ccctggggcc accagggtgg attggaacga aaatcaaagg gtcgttcaga
     8041 gatgtcagcc cgttcaaata tcctgtacct tacgccagca aaactgcctg ttctggtttg
     8101 aatatgatga acagatacca ggcttgtatc ccattctgac cacaaacctg caaagcgctc
     8161 tccttcttgc tgatggtcca gaagtgcctg atgaacctct ttcagtacca gaagctgttt
     8221 ctttacttct gttctcaaag cactttcttt tgagcggatc gttgacaaag cagattctct
     8281 gatcttcaga gctttttcaa gtccctgcaa tgctatctta ttatatgcaa tctggagtga
     8341 catcagttca tccagtatct gtcaaccaat tcctgtctga tacctgtttc atcaggttga
     8401 aaatgtttgc tgatgagctg ccatgccttg ttcagcatct cgtccatatc catttgcaca
     8461 tctatggata atagttctgc tgtataatca cgggcataag ccagggtacg tatatcgtaa
     8521 tctgtcagtt caaatccgtt ctcttttttg atcctggctt cggcagcatc tgcatacaat
     8581 ctgatacagg cgttcatgat ctgcgggtgg tcttctctgg tctgcttacc tattaccagt
     8641 tgcttgagcc tggaaagact tctgaatgga tcaataataa cctttcctgt ttcgctgtct
     8701 tttcttagaa acaactggcc ttcagttatg taacctgtgt tatccggaat ggcatgagta
     8761 atatcaccct gattcagagt ggtcactgct atgatggtaa tggagccttt gccttcaaac
     8821 tgaactgctt tttcatatat cctggccaga tctgaataca gagacccggg catactgtct
     8881 ttagatggta tctgatccat tctgttgctg actatactaa gtgcatcagc atacaacgtc
     8941 atatcagtga gcaaaaccag tacctgttcg tctttatcaa ctgcaaaaaa ctcagcagct
     9001 gtaagcacca tatcaggaac cagtaacctt tcaacgggag ggtcttcggt ggtattgaca
     9061 aaagctatga tcttatccag cacccctgca cgtctgaatg tatctttaaa aaagagcagg
     9121 tcatcattgg aaagccccat tccgcccagt atgatcttat cagctttggc acgcagggct
     9181 actgtagcca tcacttcatt gtagggttga tcaggatcag caaagaacgg gatcttctgc
     9241 cctcccagaa tggtgttgtt cagatctatg ccagctatgc cggtaaaaat aggtgtgttg
     9301 ggttgtttcc ttctgaccgg attaaccggt gggccaccga tctctctttt tcggccggtt
     9361 ggagccggcc attcatccag cggcttacca aaagcgttaa agaatctgcc acgaagtctt
     9421 tcatctactt ccaatgccgg agcagtgccc agaaaagaaa cctttgcgtc atttgcaata
     9481 ccttccgtac cactgaaaac ctgaagggtt accatgtcac cttccatttt taccacctga
     9541 gccaacctgc cgtcaaccag agccagttct tcgttcccta taccggtggc ccgcagactg
     9601 atagtagctt tggtaatatg atgaatatct gtatagaacc gattgatcac ggatgtttgc
     9661 atatcagatt gatttttcgg caggttgtat tgccatggat cttacttctt ccagaatgcg
     9721 gttaaatccc ggagattcaa agatggcata gttcaattgt ttcaggctgt tgatcatttt
     9781 tctgaaaaat ggacgtactt cagaaaaatg atcaaagtga tactctgttt gacatatttg
     9841 cagcaacaga tcagccatga attcctgacg ggtaaccgaa cagaacatat caacctcgtc
     9901 aaaagcatcc tgctgtaaca ggatgaaatc aagcagttct gatttccaga attgcatatg
     9961 gtagtcaatg cttactccat catcgcccag aatattgatc tggtctgcag cttcctttcc
    10021 ccgtagcaaa atgttcttga tctcattagc atgtgcgatc catctggtac tcacatgctc
    10081 cttcatataa tctctgaact caggatactc caggtacttt gaataggaat ctatcgggtc
    10141 aactgcagga tatcttttgc tatcggcacg tctttgtgaa agtgcataga aacatctggc
    10201 tgctttcttg gtagcttctg ttacaggttc tttcaggtta cctccggccg gagataccgt
    10261 accgataaaa gtgattgaac cctgctttcc atttgtcagt tccacatatc cggctcttgc
    10321 ataaaaagct gctatggtag cggtaagatc cataggaaaa gcgtcaggcc ccggcagatc
    10381 ttccattcgg ttagacattt cacgaagagc ctgtgcccag cgtgatgtgg aatctgccag
    10441 caacaatacc cgcagcccca tattccggta gtattcacaa atggtcatgg ctgtatagac
    10501 gctagcttct ctggcagcta caggcatgct ggatgtattg gctatgatga tggtcctttc
    10561 gatcagtttt cgtcctgtgg ccgggtcatc cagttcagga aactctgtaa agatctccac
    10621 cacttcattt gctctttcac cacatgctgc aatgactacg atatctgctt tcgcctgctt
    10681 ggcaatggct tgctgcagta cggttttgcc gcaaccgaat ggtcccggta taaatcctgt
    10741 acctccctcc agcattgggt gcacggtatc aattgttctg atgccggttt ccagcatttt
    10801 gaacgggcga atttttctac catacgcttt gagtgccaat ttacttggcc aggtgctaac
    10861 cattgttact gcatgttttc tgccatcagt catgatgatc tcagcgactg tatctgctgc
    10921 tttatattct ccagccggtt tgatctcagc tattttcccc tgaccttcca tactgaaggg
    10981 agccatgatc ttgtgcggca gtccattctc tgttatttct ccaagccagg ctccggcaga
    11041 taccatgctt ccagtctgaa ccaatggtct gaaatcccat ttttgatgta catccaatgc
    11101 aggagaacgc atgccacgaa gcaggaatgt gccttcgtgg gcatcgagat cactttgcaa
    11161 tccgtcgtat tttttaccca gcatacccgg accaagcgtc acttccagca gatcgccggt
    11221 aaagttcact tctgccccga tgcgtactcc tcttgtgcta tcatacacct gcacaaaagc
    11281 gatctcacca ctgatcttaa tgatctcacc catcaacaga tcttcgcccg cctgcaggta
    11341 gcatatttcg ttttgtctga caggtccgtc aggttttacc tgcaccagat tggcaatcag
    11401 accctgaact gtaccacgaa ctttcataca tatgtattta catcaatgtc aaataccgga
    11461 accttggcgt cagcatttct gatcctgctc catcttgcta tcagttgttg ttgccatgca
    11521 taggccaata atcggtcaat tcgaaattct ttctgaaaga gccgttcttc aagccagttc
    11581 caggcaaggt tgtcagcttc ccattccagt tcttccgggt gtctgatggt cattaaacgc
    11641 atgatctgtc ctgaaatgcc ggttgcttcc agtatccggc gatggtaatc tggccaggaa
    11701 atgttcggtt cttccacacc ggaaagccgg tgaacatctg caatggcatc accattgtct
    11761 tctacaagca ggatcctgat caggaacaga gcctcctgta aaacggtgta ttcatgaagc
    11821 aaccggttgt ttgtctgacg aacaaaggtg gtccaggcga tcctccagga tgcctccagc
    11881 gatatcctgg gcgattcttt gccgggaaat agcatttgat cctggtaggg aaataatttc
    11941 aggttttgct gcaggt
//
LOCUS       DAOMAX010000059        11923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_691_length_11923_cov_4.221014, whole genome shotgun sequence.
ACCESSION   DAOMAX010000059 DAOMAX010000000
VERSION     DAOMAX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_691_length_11923_cov_4.221014"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PK742_04855"
     CDS             complement(<1..189)
                     /locus_tag="PK742_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HQU75954.1"
                     /translation="MKRNLLVVSTSTVHGSPYLSYIMSEVQDFFRDVDEIIFVPYARP
                     SGVSWESYSAKAAAAFAEA"
     gene            complement(192..1430)
                     /locus_tag="PK742_04860"
     CDS             complement(192..1430)
                     /locus_tag="PK742_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU75955.1"
                     /translation="MKTPGHHRPLTSRILNATVIVAALGYFVDIYDLILFGIVRGPSL
                     RALGYDGQELVDKGLFLLNMQMAGMLLGGLLWGILGDMKGRISVLYGSIFLYSIANIL
                     NAMVVSIDQYALLRFLAGVGLAGELGAGITLVNETMTKEQRGYGTMVVVTFGALGAVV
                     AAMIADNFQWQTSYYVGGALGLALLLLRIGTYESGMFRKVQEKGTGLSFLKLFASRDR
                     LYKYFLCILIGIPIWFVIGILVILSPELSKVIGVRGEVQAGITILWCYIGLSAGDFAS
                     GMISQLIKSRKKVVLYYIIGCTGVILLYLYSRDVSLWYFYFLSFVLGFSAGYWALFVT
                     MASEQFGTNIRSTVTTTVPNFVRGSVIPVTISYQYLAGKFNNVDGAAVVGMICMLIAF
                     LSLLPLKDTYGKDLDYMETI"
     gene            complement(1427..1945)
                     /locus_tag="PK742_04865"
     CDS             complement(1427..1945)
                     /locus_tag="PK742_04865"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQU75956.1"
                     /translation="MNLSIIVAVSENGVIGLNGDMPWKKLPSDLKFFKETTMGHWCIL
                     GRKTYDALGGKVLPGRRFIIITRDTAYQSADSLVVHSVFEAMHHPAIADEKQVFVLGG
                     GDIYRQLRPYCNTIYLTRIHASFDGDTYFNTPDEEEWILAERVSVQQDEYNPYDHDFL
                     TYKRKGSDTGKV"
     gene            complement(1942..2736)
                     /locus_tag="PK742_04870"
     CDS             complement(1942..2736)
                     /locus_tag="PK742_04870"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQU75957.1"
                     /translation="MQQYHDLLRRILDEGVAKSDRTGTGTVSIFGHQMRFDLSEGFPL
                     LTTKKLHVRSIIHELLWFLQGNTNIAYLRDNNVSIWDEWADEDGNLGPVYGHQWRSWT
                     TAEGNVIDQISQVVEQIKTNPDSRRLIVSAWNVGDIPKMALPPCHLLFQFYVADGKLS
                     CQLYQRSADTFLGVPFNIASYALLTMMMAQVTGYQPGDFVHTLGDTHLYNDHLEQARL
                     QLSREPRKLPRMVINPDVRDIFSFRFEDFTLQDYDPHPHIKARVSV"
     gene            complement(2850..4160)
                     /locus_tag="PK742_04875"
     CDS             complement(2850..4160)
                     /locus_tag="PK742_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75958.1"
                     /translation="MRIHIAFVILYGFICTTGQAQQTSISMQFKPRMELRDGYQTLPV
                     DGTTPAFHTNTRVRLQMGYTSNRVEVVAQPQYIYVWGQNSFNSSADNNFSLYQAYAQL
                     NLAGKTIDSTAAIDKVAFRIGRQELNYDGGRILSNLDWAPQGIRHDAALFILEDGVWS
                     LHAGAGYNQDGPANFGSNFSKNYYKSFQMVRLHREGKAFKGSLLFFKDDFQQFETDSL
                     GIPYSTEVTGRINAGTWLSYAISPQLSVEGEYYVQKGKDPTGNDLNAWLMNAQLVWKA
                     IEGKLVITPGIDIVSGNDGLEADPVNHAFNPTYGINHKFYGFMDYFYTSSPFGSGGLQ
                     DIFVRTSYKINNDWNTWVHFHYFASAGNINDVSDPGNAADPYFGTEVDWVLNYRFDQT
                     TQLQFGAATMLGSSTLQQVKGQPATDGGMGFFSYLQFQFTPKFL"
     gene            complement(4273..5973)
                     /locus_tag="PK742_04880"
     CDS             complement(4273..5973)
                     /locus_tag="PK742_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQU75959.1"
                     /translation="MSWVDWIVLMCTLAFIVGYGIYRTRRSNSMEAYLRGNNENRWWE
                     IGLSVMATQASAITFLSTPGQAYEDGMRFLQFYFGLPLAMILVAVFFIPVFYKLRVYT
                     AYEFLEDRFDVKTRSLTAILFLVQRGLAAGITIYAPSIIISQLIGWPLNTTILFIGVL
                     VTMYVLTGGTRAVSVTHKQQMAVIFLGLFTALGFLLWHLHHAGGVLNSLKVAGVTGRM
                     QLIDWKWDPQNRYTIWSGLIGGLFLQLSYFGCDQSQVGRYLGGRDVKESRLGLMFNGL
                     MKIPMQFLILLTGVMVWVFYLTHTPPVWFDQQAYTQANTPAYAAELQESAELWNTSYI
                     KQQQEVRKAISIIQENGDLEAGQRSALKESIEAQESARDQVGSLIRTADPDAGDDHDY
                     IFMTFVLQVLPIGLIGLLFAVIFSASMSSTSSEINALAGTFAVDIYKRLLKRDAEDKH
                     YVLASRLFTLLFGALAITFALVASLFDNLIEAVNIIGSLFYGTILGIFLTAFLLKRVK
                     GHAVFTAALITQCIIFYLDFSVRYSWPLPSAEIGYLWYNVIATGLVMVFSAILNRFFR
                     "
     gene            complement(6091..8607)
                     /locus_tag="PK742_04885"
     CDS             complement(6091..8607)
                     /locus_tag="PK742_04885"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQU75960.1"
                     /translation="MHLKLYSSLLLCGLSAGLSAQQAQMDPSEIYASLQKMSQTTRVL
                     YIAAHPDDENTRLLTFLSRGKHFETAYLSLTRGDGGQNLIGDEQGADLGVVRTQELLA
                     ARRVDGTRQFFTRAIDFGYSKSAQESLSLWQHDSILSDVVWVIRQFQPDIIITRFPPD
                     ERAGHGHHTASAMLALEAFDLAADPGAFPEQLKWCEPWKVERVFWNASSWWDKTLPDQ
                     VDNKTVFGVDVGGYDPILGMSYGELAGKARSQHRSQGFGVSPYRGEQIEYLRLLKGEL
                     QPGSTDPFEADSHWAKMVNGTETRSLLVKMLEEYDMKDPLAALPDLLKMYALVPSEDR
                     TPQWTDKQNRLSDIIAASVGLWLEPVVIAKETDRIQISAECLSAAGVSATVTGMDLYF
                     PGDGNDLFPMLHPRGKAAQISSEKQQLPANIFVRDTFWIAVTPGANATPYWLQRPYGT
                     TYQAADAKWRGQPEWDDLPAVRFRIQLGESELAFTRKLVEKKIDPARGEYYVPVQMLP
                     AYHVHVEEQLCLFKPGLPSEVHVKLDGLDAETTGDLKLRIPSGWSVSPASIRIQGNEH
                     TLYTFQVTASDNGSEGVITPVFHSHGNDYEQEITDIIYEHLPEQHIAHIASRPAMVLL
                     MHVPEGSYAYIEGAGDLVLESLQAAGVNVEPLSVNDLAVTDLSVYDAIITGVRAYNVS
                     DRLISAQPLLLQYVQQGGTLLVQYNTTNNFTLGRNADGASMGPYPFSLTRGRTTDENA
                     SPEFLLPDHPVWNTPDKITADDFEGWVQERGLYYADATADYYQAPLGFADPGEETLRG
                     AILTTDYGQGTFIYTGLSFFRQLPAGVPGAYKLFFNMMTY"
     gene            complement(8659..9030)
                     /locus_tag="PK742_04890"
     CDS             complement(8659..9030)
                     /locus_tag="PK742_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75961.1"
                     /translation="MKRRRTTLLYPAWALAMFLSFLSGYALGNSQIQSVPPDQQEEWK
                     GSLPETTSRNSITVPESTKDIVIVFTVDEGNRIHVLDISGGKNPVTDYIKRSLEGRVI
                     GSPGAVPGINYVMTLRFPSSV"
     gene            9174..9962
                     /locus_tag="PK742_04895"
     CDS             9174..9962
                     /locus_tag="PK742_04895"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU75962.1"
                     /translation="MELNGKTVWITGASSGIGAALAVALSVKGCSLILSARRREQLQE
                     VAGQCHTKAMIVVMDVSDQKSIIGAWQEIQASGGMPDILINNSGISQRSAALETQIET
                     DRRIMEVNYFGAVTLTKLVAPYLVQKGSGMIVNISSLSGKFGWKLRSSYAASKFALLG
                     FFESLRAELEGSGVEVLNVIPGRIRTEIAQHAVLGDGSAYAKSDRGQETGIPVEVCAG
                     KIVRAMKKNKKEIVIARIDGLMIHIRYWMPWLYYRIAAKRDPNK"
     gene            9985..11049
                     /locus_tag="PK742_04900"
     CDS             9985..11049
                     /locus_tag="PK742_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQU75963.1"
                     /translation="MSQLPVISGIQQIGIGNPYVHEAWKWYRIHLGMDIRIFEEAAEA
                     NLMLPYTGGQPHKRHAVLAINLNGGGGFEIWQYTSRKPVAADFPIQFGDLGIYGCKIK
                     SRDVQATYNQMKKSGVEVLGDVSTDPAGRLHFFLRDLYGNVFEVVPSDNWYTRQRALT
                     GGAYGTIMGVTDMDRSIRFYKEILGYDQVIYDVQGRFDDLQVLPGGNTEVRRVLLTHS
                     DDRKGPFSRMLGRSEIELMSISGRTPRKIFENRFWGDLGFIHLCFDIVGMQAMEEKCR
                     KLGHPFTVNSAESFDMGEAAGHFTYIEDPDGALIEFVETHKVPVLKKLGWYIHLKDRD
                     PEKPLPDWMLKALRFNRKSD"
     gene            complement(11046..11546)
                     /locus_tag="PK742_04905"
     CDS             complement(11046..11546)
                     /locus_tag="PK742_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75964.1"
                     /translation="MNIREASMDMVDRLKAHIALMDADQYAEKLEVLNGSSVGDHFRH
                     IIDFYLCIGNGLEAGLVSYDNRERDILISQCPLYASGKLNDIRETIAVLDPEKSLLVA
                     QSFSTDKTVPAVKLQTTVGRELMYAYDHAVHHMAIIAIGIRVHFSDLQMEEGFGLAPS
                     TIRNLS"
     gene            complement(11614..>11923)
                     /locus_tag="PK742_04910"
     CDS             complement(11614..>11923)
                     /locus_tag="PK742_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75965.1"
                     /translation="SVRLQWLVRQKKLAYYSRVAVNKLTNRWTFRLYFTDYDKWIRNY
                     HNDWFRKPFVHIHSDSGVDPEKALAFTNGYLKGDRSFAYYKLLTNLFSTLHFIQQSDH
                     "
BASE COUNT         3268 a         2884 c         2801 g         2970 t
ORIGIN      
        1 ggcctctgca aatgcggctg cggctttggc agaatacgat tcccatgaaa ctccggaagg
       61 tctggcatag ggtacaaaaa tgatctcatc cacatctcta aaaaagtcct gcacttcaga
      121 catgatatat gacagatagg ggctgccgtg tacggtggaa gtgctgacca ccaaaagatt
      181 tcttttcata gtcaaatagt ttccatataa tccagatcct taccgtaggt gtctttcagt
      241 ggcaacagcg acaggaacgc aataagcata cagatcatgc ccacaactgc cgctccgtca
      301 acgttgttga attttccggc cagatactga taagagatgg ttaccggtat gaccgatccc
      361 cgaacaaaat tgggaacagt tgtagtgacg gtcgatctga tatttgttcc aaactgctct
      421 gaagccatgg taacgaaaag cgcccagtag cccgcactga aaccgagaac aaagcttaga
      481 aaataaaaat accagagtga aacatcacgg ctgtacagat acaacagaat gaccccggta
      541 catccgatga tgtagtacaa gacgactttc tttctgcttt tgatcagctg actgatcatt
      601 ccgcttgcaa aatcgcctgc tgacaatcct atatagcacc aaagaatagt aattcctgcc
      661 tgcacttctc ccctcacacc tatgaccttg cttagctctg gagaaagaat gaccagtatt
      721 ccaatgacga accagatggg aatacctatc aggatgcaca gaaagtattt atataatctg
      781 tcgcgactgg caaacagttt caggaaagaa agacctgttc ctttttcctg cacctttctg
      841 aacattcctg attcatatgt accaatccgc agcaacaaca aagcaagtcc aagtgcgcca
      901 cccacataat aggaggtctg ccactggaag ttatcagcga tcatggcagc taccacagca
      961 cctaatgccc caaaggtgac cactaccatg gtgccataac cacgttgttc tttggtcatc
     1021 gtctcattga ccagagtgat cccggctccc agctcacctg ccagtccaac tccggccagg
     1081 aaccgaagca gagcatactg atcgatgctg accaccatcg cattgaggat attggcgatc
     1141 gaatacaaaa agattgaacc gtagagcaca gatatcctgc ctttcatatc gcccaggata
     1201 ccccacaaca atccgcccag cagcataccg gccatctgca tattgagcag gaacaagcct
     1261 ttgtcaacca gttcctgtcc gtcatatccc agagcacgaa gacttggacc ccggacgata
     1321 ccaaacagga tcagatcata tatgtcaacg aaatatccca gagctgccac gatcacggtg
     1381 gcattcagaa tgcggctggt aagcggccga tggtgccccg gtgttttcat acttttccgg
     1441 tatctgatcc cttcctcttg taggtcagaa aatcatgatc gtaaggatta tattcatctt
     1501 gctgaacaga cacccgctca gccaggatcc actcctcttc atcgggtgta ttgaagtagg
     1561 tatctccatc aaaactggca tgtattcgtg tcaggtagat ggtattgcag tacgggcgaa
     1621 gttgtctgta gatatctcct ccgcccagaa caaagacctg tttctcatca gcgatggccg
     1681 ggtggtgcat ggcttcaaag acagaatgta ccaccaggct atctgcagac tgataggcag
     1741 tgtctcgtgt aatgatgata aatcttcggc cgggtaagac cttcccgcca agagcgtcat
     1801 aagttttacg tccaagaatg caccagtgcc ccatggtagt ttctttgaag aatttcaggt
     1861 cagaaggaag cttcttccag ggcatatctc cattcaggcc tattacgcca ttctcgctga
     1921 cggccacaat gatgctcaga ttcatacgct caccctggct ttgatgtggg gatgcggatc
     1981 gtagtcctgc aaggtaaaat cttcaaaacg gaaagaaaaa atatctctca catcaggatt
     2041 gatcaccata cggggcagtt ttctaggttc gcgggaaagc tgcaaccttg cttgttcaag
     2101 atgatcatta tacagatgcg tatcgccgag ggtatgcaca aaatcaccag gctgatagcc
     2161 ggtaacctgt gccatcatca tggtcagcaa agcatacgat gcaatattga acggaacacc
     2221 taaaaaagta tcagcactgc gctggtaaag ttggcaggaa agtttgccat ctgccacata
     2281 gaactgaaac aggaggtggc agggggggag ggccatttta ggaatatcac ccacattcca
     2341 ggctgaaacg atcagacgtc tggaatcagg gttcgttttt atttgctcca ccacctgtga
     2401 tatctgatct atcacattcc cttcggcggt tgtccagctg cgccactgat ggccgtaaac
     2461 aggcccgagg tttccatctt catctgccca ttcatcccag atgctcacat tgttatcacg
     2521 caggtaggca atattggtat taccctgcag aaaccacagc agttcatgaa tgatggatct
     2581 tacatgaagc ttcttggtgg tgagcaacgg gaatccttca gacaggtcaa agcgcatctg
     2641 gtgaccgaaa atactgacgg ttccggtgcc ggtgcgatcg cttttagcga caccctcatc
     2701 caggatccgg cgcagcagat catggtattg ttgcatgttg taaaattaga aggaatgtgt
     2761 accaaccaca acaacgcctg cggcagttta aacagtttac ccacaaaaaa aggcgaccaa
     2821 caggccgcct tgtatttgca tgagaatagt tacaggaatt ttggggtaaa ctggaactgg
     2881 agatagctga agaatcccat acctccgtcg gttgcaggtt ggcccttgac ctgctgcaag
     2941 gtagaagaac ccagcatggt ggcagccccg aactgcagtt gtgtggtttg atcaaagcgg
     3001 taattcagca cccagtcaac ttcggtaccg aagtaaggat cagcggcatt acccggatcg
     3061 gatacatcat tgatattgcc tgcacttgca aaataatgga agtgcaccca tgtattccag
     3121 tcattattga tcttgtaaga ggtgcgcaca aaaatatcct gcaaaccacc actgccaaaa
     3181 ggacttgaag tatagaaata atccataaat ccataaaact tgtggttgat accataagta
     3241 ggattaaatg catggtttac cggatcagct tccagaccgt catttccgga tacaatatca
     3301 ataccaggag taatgacaag tttaccttca attgctttcc agaccagttg tgcattcata
     3361 agccaggcat tcaggtcatt tccggtgggg tctttgcctt tttgcacgta gtattcacct
     3421 tccacactaa gctggggtga aatggcataa gacaaccatg taccagcatt gatcctgcct
     3481 gttacctcgg tagaataagg aatgcccagt gaatcggttt caaattgctg gaaatcatct
     3541 ttaaaaaaca acaaagagcc tttaaaggcc ttcccttccc ggtgcagcct taccatttga
     3601 aaagatttat agtaattctt tgaaaaattg cttccaaaat tggcaggtcc gtcctggtta
     3661 taacctgcac ctgcatgtaa agaccagaca ccatcttcca gaatgaacaa tgcggcatca
     3721 tgtcttatac cctgaggtgc ccagtcgagg ttgctcagta tccttcctcc gtcgtaattc
     3781 agttcctgac ggccgatacg gaaagccacc ttatcaatgg cagctgtgct gtcaatggtt
     3841 ttccctgcca ggttcaactg tgcgtaggcc tgatacagag agaagttatt gtctgctgag
     3901 ctgttaaagg aattctgccc ccatacatat atatattgtg gctgggccac cacttctact
     3961 ctgttggagg tataacccat ttggagccgc accctggtat tggtgtggaa ggccggtgtt
     4021 gttccgtcaa ctggtaaggt ctgatagcca tcacgaagct ccatacgggg tttgaactgc
     4081 atggaaatac ttgtttgctg agcctgtccg gttgtgcaaa taaatccgta aaggatcacg
     4141 aatgcaatgt gaattcgcat aggtattcag attttggatt tgcaaaacta tctgacctca
     4201 ggataggttt tgtaaacgtt tttcaaaaca ttgtaaacga catgtaaaca gatccagtca
     4261 aataagtggg ctctaccgga aaaatcggtt cagaattgca gaaaacacca ttaccaaccc
     4321 tgttgcaatg acattatacc acaggtatcc tatttctgca gaaggcaatg gccagctgta
     4381 acgaacgctg aagtccaggt aaaatatgat acactgggtg atgagggccg ctgtaaaaac
     4441 tgcatgtcct ttaacccgct taagcagaaa ggctgtaaga aaaataccga gtatggtacc
     4501 atagaacaga cttccgatga tgttcacagc ttcaataaga ttgtcgaaca aggaagcaac
     4561 aagggcaaaa gtgatagcca atgccccaaa caagagggta aataatcttg aagccagcac
     4621 ataatgttta tcttctgcat cacgtttcaa caatctctta tatatatcta ctgcaaatgt
     4681 gcctgccagt gcattgatct cagaggaggt actgctcata cttgcagaaa agatcactgc
     4741 aaacaacagt cctatcaggc cgataggcag cacctgcaac acaaatgtca taaagatgta
     4801 gtcgtggtca tcgccggcat ctggatcagc agtcctgata agcgatccaa cctgatcacg
     4861 ggcgctttcc tgtgcttcaa ttgactcttt cagcgcagag cgttgcccgg cttccagatc
     4921 accgttctcc tgtatgatgg agatggcctt acgtacctcc tgctgttgct tgatatagga
     4981 ggtgttccat agttcagcag actcctgcaa ctcagctgca taggcaggtg tgtttgcctg
     5041 agtatatgcc tgctgatcga accagacagg aggtgtatgt gtcaggtaaa acacccatac
     5101 catcactcct gtcaggagaa taaggaactg catcggaatc ttcatgagcc cgttgaacat
     5161 gagtccaagc cggctctctt tcacatcgcg accacccaga taccgaccta cctggctctg
     5221 atcgcagccg aaatagctca gctgcaggaa aagtccgcct atcaatccgc tccagatggt
     5281 atatctgttt tgcggatccc acttccagtc aatgagctgc atcctgcctg ttacaccggc
     5341 tactttcagg ctattcagaa ccccgcccgc atgatggaga tgccagagca ggaaaccaag
     5401 cgctgtgaac aatcccagaa agatgactgc catttgttgc ttgtgggtga ccgatacagc
     5461 tctggttcca cccgtaagca catacatggt aaccagcaca ccgatgaaca gaatggtggt
     5521 gttgagcggc catccgatca gctgcgaaat gatgatgctg ggagcataga tggttatacc
     5581 ggcagccagc ccgcgttgta ccagaaaaag tatggctgtg agagaacggg tcttgacatc
     5641 aaaacggtct tccagaaatt cataagctgt ataaaccctg agcttataga ataccggaat
     5701 aaaaaacacc gccaccagga tcattgcaag tggcagccca aaataaaact gaagaaatct
     5761 catgccatct tcataagcct gaccgggtgt ggacaggaaa gtgatggccg aagcctgtgt
     5821 agccatcaca ctcaggccga tctcccacca acggttttca ttatttccac gcaggtaggc
     5881 ttccatgctg ttggatctgc gggtgcgata aatgccataa cccacaatga atgccagggt
     5941 gcacatcagc acgatccagt caacccagct catgaaaaca catttgtaat acaagtatat
     6001 aatatgatct gcagaagcag aaacagcagc aatgcgatgt accagcgccg ccagtaagtt
     6061 tgttcctttg gatcaatact cattgacagc ttaataggtc atcatgttaa aaaatagttt
     6121 gtatgctccc ggtactccgg ccggcaactg ccggaaaaaa ctcaggccgg tataaatgaa
     6181 ggtaccctgt ccatagtcag tggtcaggat cgctccgcgt agcgtttctt cgccgggatc
     6241 tgcaaaacca agtggtgcct gataataatc tgcagtggca tctgcataat acaaaccccg
     6301 ttcctggacc catccttcaa aatcgtctgc tgtgatcttg tctggtgtat tccagaccgg
     6361 atgatcaggc agaagaaatt caggactggc attttcatct gtggtcctcc cccgggtcag
     6421 actgaatgga tacggaccca tagaagcccc gtctgcattc cggccgaggg taaaattatt
     6481 ggtagtgttg tactgcacca gcaatgtacc gccctgttgc acatactgca aaagcagtgg
     6541 ctgagcactg atcagacgat ctgaaacatt gtaagcacgc acaccggtga tgatcgcatc
     6601 gtacacggaa aggtcagtaa cagccagatc atttactgac aatggctcca catttactcc
     6661 tgctgcctgc agtgattcca gaaccagatc gcctgcacct tctatatatg catagcttcc
     6721 ttcgggcaca tgcatcagaa gtaccatggc aggtcttgag gcaatatgcg caatatgttg
     6781 ttcgggcaga tgttcatata ttatgtcagt gatctcctgc tcataatcat taccgtggct
     6841 gtgaaataca ggtgtaatga ccccttcact gccattgtct gatgctgtta cctgaaaagt
     6901 gtacaacgtg tgttcattgc cctgaatccg gatggaagca ggagacacac tccagccgga
     6961 cggaatgcga agcttcaggt cacctgtggt ttcagcatcc aggccatcaa gttttacatg
     7021 cacttcagag ggtaatcccg gtttgaagag gcacaattgt tcttccacat gaacatgata
     7081 tgcaggcagc atttgcacag gcacatagta ctctccgcgt gccgggtcga tcttcttttc
     7141 aaccagtttt ctggtaaagg caagttctga ttctcctaac tgaatacgaa aacgtaccgc
     7201 cggcagatca tcccattcag gctgaccccg ccattttgca tctgctgcct ggtaggttgt
     7261 accataaggt ctttgcagcc agtagggggt agcattggct ccgggagtaa ctgctatcca
     7321 aaaggtgtct ctcacaaaaa tattcgccgg tagttgttgt ttttctgaac tgatctgagc
     7381 tgctttcccg cggggatgca acataggaaa caggtcgttt ccatcgcccg gaaaataaag
     7441 atccatacct gtaacagtgg ctgacacacc tgctgccgac aagcattcgg cagagatctg
     7501 aatcctgtca gtttcctttg ctatcaccac cggctccagc cataatccga ctgaagccgc
     7561 aatgatatct gacaaccggt tttgtttatc tgtccattga ggtgttcggt cttccgacgg
     7621 aacgagggca tacatcttca gcagatccgg tagtgccgcc agaggatcct tcatatcgta
     7681 ttcttccagc attttgacca gtaatgaccg tgtttctgta ccgttgacca tcttcgccca
     7741 gtgtgaatcg gcctcaaaag gatctgtact accgggctgc aattctccct tcaacagcct
     7801 gaggtactcg atctgttctc ctctgtaagg agatacgccg aacccctgac tgcgatgctg
     7861 gctgcgggct ttccctgcca gttctccgta tgacatgcca agtatcggat catagccacc
     7921 aacatctaca ccaaaaaccg ttttgttatc tacctgatca ggaagggtct tatcccacca
     7981 ggaggaagca ttccagaaca ctctttccac cttccatggt tcgcaccact tcagttgctc
     8041 cggaaatgcc ccggggtctg ctgccagatc aaaagcttcg agtgccagca tggctgatgc
     8101 cgtatgatgc ccgtgcccgg ctctttcatc aggtggaaac cgcgtaatga tgatatcagg
     8161 ctgaaactgt ctgatgaccc aaaccacatc agaaagaatg gaatcatgtt gccacagaga
     8221 caatgattcc tgtgcacttt tggaatagcc gaagtctatg gcccgggtaa agaactgcct
     8281 ggtaccgtcc acccgccgtg ccgccagcaa ttcctgggtg cgcactactc cgaggtctgc
     8341 accctgttca tcgccgatca ggttctggcc tccatcacca cgcgtaagag acaaataggc
     8401 tgtttcaaag tgtttccctc ttgacaggaa agtaagcaaa cgggtattct catcgtcagg
     8461 atgcgctgca atatagagca ccctggtagt ttgactcatt ttttgcagag aagcatagat
     8521 ctctgacgga tccatctgcg cctgttgtgc tgatagccca gcagacagtc cgcataaaag
     8581 cagtgaggaa tatagtttaa gatgcacacc ctaacatttg gtatgcggta aaaataagaa
     8641 tacgtgcggg aaggggtgtc agacagaaga ggggaatctg agtgtcataa cgtaattgat
     8701 gccgggaacg gctcccggag atccaatgac ccgaccctcc agagatcgct tgatgtaatc
     8761 tgtaacagga ttcttaccac ctgaaatgtc cagcacatga atacgattac cttcatctac
     8821 ggtaaacaca atgacaatat cttttgtaga ttcaggaaca gtaatactgt ttctggaggt
     8881 cgtttcaggc aaactacctt tccactcttc ctgttgatcc ggtggaacac tttgtatctg
     8941 gctgttaccc agggcatacc cggatagaaa gctcagaaac atggccaggg cccaggcagg
     9001 atacagcaaa gttgttctgc ggcgcttcat agtggttttc tgctcatgtg aaattaacag
     9061 ttgatacatt ccagaacaag gatttaacca agtttaacca tatgacgcac aaacgttagt
     9121 aattgttaca gcaagtgcaa aaaaaaagtc agttttggca acattctact attatggagc
     9181 taaacggtaa gacagtctgg atcacaggag cctcttcggg catcggggcg gcattggcag
     9241 tggccttgtc tgtgaaaggt tgcagcctca ttttatcagc aaggaggcgg gagcagctgc
     9301 aggaagttgc cggtcagtgc cacactaagg ctatgatcgt ggtcatggat gtatctgacc
     9361 agaaatcaat tatcggcgca tggcaggaaa tacaagcatc aggtggtatg cctgatattc
     9421 tcatcaataa cagcggtatc agccagcgtt ctgctgcgct ggaaacacag atagaaaccg
     9481 acagacggat catggaagtg aattacttcg gtgctgtaac acttactaaa ttagttgctc
     9541 cttatttggt gcagaaaggt tcaggcatga tcgtcaacat cagcagtttg tctggaaaat
     9601 tcggttggaa gctgcgcagt tcctatgctg ccagcaagtt tgccctgctg ggcttctttg
     9661 agtcacttcg tgctgaactg gagggttcgg gtgtggaggt gctgaatgtt attccgggcc
     9721 gcatccgcac cgaaattgct caacatgcag tcttaggcga tggaagcgcc tatgccaaat
     9781 cagaccgtgg tcaggaaaca ggaatcccgg tggaagtttg cgccggcaag atcgttcggg
     9841 cgatgaaaaa aaataaaaaa gagatcgtaa tagcacgaat agacggtttg atgatccaca
     9901 tacgctactg gatgccctgg ctttattacc ggatcgccgc caaacgtgat ccgaacaagt
     9961 aggatagttg gtatttttgc cggcatgagt caactacctg taataagtgg cattcagcag
    10021 atcgggattg gtaatccgta tgtacatgaa gcatggaagt ggtatcgcat ccatctgggt
    10081 atggacatca ggatctttga agaagcagcg gaagccaatc tgatgcttcc ctacaccgga
    10141 ggacagccgc acaaaagaca tgctgtactg gccatcaacc tgaatggcgg gggtggattt
    10201 gagatctggc aatacaccag cagaaaaccg gtggctgcag attttcccat tcagttcggt
    10261 gatctgggta tatatggctg taagatcaaa agccgcgatg tacaggccac ttacaaccag
    10321 atgaaaaaga gtggtgtgga agttctggga gatgtgagta cagaccctgc aggaaggctg
    10381 catttttttc tgagagacct gtacggaaat gtttttgagg tggtgccttc tgataactgg
    10441 tacacccggc aacgagcctt gacaggtgga gcctatggta ccattatggg cgttaccgat
    10501 atggaccgtt ccatcaggtt ctataaggag atactggggt atgaccaggt gatctatgac
    10561 gtgcagggaa gatttgatga cctccaggtg ctgcccggtg gtaatacaga agttcgtcgt
    10621 gtgttactta cgcatagtga tgacagaaaa ggtccgttca gcaggatgct ggggcggtca
    10681 gagatagagc tgatgtccat ctcaggcaga actcccagaa agatctttga gaacaggttc
    10741 tggggcgacc ttggattcat tcatctttgt tttgatatag tggggatgca ggctatggaa
    10801 gaaaaatgcc gcaaactagg tcatcctttc accgtcaaca gtgccgaaag ttttgacatg
    10861 ggagaggctg ccgggcattt tacttacata gaagaccccg atggtgcact cattgaattt
    10921 gtagagacac acaaggtacc ggtactcaag aaactgggct ggtatataca cctgaaagac
    10981 cgcgatccgg aaaaaccact gccagactgg atgctgaagg cacttcgctt caacaggaag
    11041 agtgattaag aaaggttgcg gatggtggaa ggtgccaaac caaatccttc ttccatctga
    11101 agatcagaaa aatgcacccg gattccgatg gctatgatgg ccatatgatg cacggcatgg
    11161 tcataggcat acatgagttc tctgcccaca gtggtttgaa gtttcacagc cggtacagtc
    11221 ttgtctgtac tgaatgactg agccaccagc agagactttt cagggtccag tactgctatt
    11281 gtttcacgga tatcgttcag cttgccagat gcatacaacg gacactggct gatcagaata
    11341 tccctctcac gattatcata agaaacaaga cctgcctcca agccattgcc aatacacaga
    11401 taaaaatcaa ttatgtgtct gaaatgatct cctactgatg acccattcag cacctcaagc
    11461 ttctctgcat actgatcagc gtccatcaaa gcaatgtgtg ctttgagcct gtcaaccatg
    11521 tccatcgaag cctctctaat attcatcata caaaaataca ggctagaaca gcgctccgta
    11581 tcatagggtt cacattcatt tgtaaaaaaa tccctaatga tcagactgct gaataaaatg
    11641 aagtgtgctg aaaaggtttg tgagtagttt gtagtaagca aatgatcggt cgcccttcag
    11701 gtatccgtta gtgaatgcca gggctttttc cggatctact ccactgtctg aatgtatgtg
    11761 cacgaaaggc tttctgaacc agtcgttgtg atagttccgg atccacttat catagtctgt
    11821 gaaatacaag cggaaggtcc atcggttggt aagtttgttc actgcaaccc gtgaataata
    11881 agcaagtttt ttctgtctta ccagccattg caggcgaaca ctt
//
LOCUS       DAOMAX010000060        11748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_710_length_11748_cov_4.572223, whole genome shotgun sequence.
ACCESSION   DAOMAX010000060 DAOMAX010000000
VERSION     DAOMAX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_11748_cov_4.572223"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..717)
                     /locus_tag="PK742_04915"
     CDS             complement(94..717)
                     /locus_tag="PK742_04915"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HQU75966.1"
                     /translation="MRWFVLSVLPLIWSCERPDTIAPRITLLPDPQPVHYLDEPYVDP
                     GASVLDNLTCNTEGMLYTDSQVDVSHYGSYQVIYTATDEAANSQTAVRDVDIVLATTN
                     YHHLYYQATDTCTSGIYTYVGLIQDCDCEGQEVTVANISNFGLSATFDLPVTGTYNEI
                     LELDTLKTGVYFLGNATMSSGADTLFWTYTITADGDEDVCHSQWVKE"
     gene            complement(780..1445)
                     /locus_tag="PK742_04920"
     CDS             complement(780..1445)
                     /locus_tag="PK742_04920"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HQU75967.1"
                     /translation="MKRMKILVPALFLGAGVLFMTGCGTDDLDAPVVTLNGDNPQIID
                     LGDAYTEAGAIATDNEDGDISASIVTDATGVNTAMVGKYTVTYTVVDEAGNTGTAERD
                     VWVRATADDYVGFFTVTENCIGSIDPYEINIVKINATTVRVSNLGDFDAGETLFDMEL
                     SGDLNDVVTINDSDDNDATLFCTASGSLVDGMSGAMHFNVSYTFDDGTDSFTCSDVDI
                     EQN"
     gene            1544..1936
                     /locus_tag="PK742_04925"
     CDS             1544..1936
                     /locus_tag="PK742_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75968.1"
                     /translation="MKIRNLAIIFSLGLLVASCTDKKAAEEIEKLKMDMAAADSLCQA
                     DKAMLMDSIANLHMMWEEMSAPANKTSSGSTKSSTSTTTTTTDTKSDDKSVNDRGGST
                     QVEKKVTDRGGSDAEKTVKKDINKRGGN"
     gene            complement(1984..3324)
                     /locus_tag="PK742_04930"
     CDS             complement(1984..3324)
                     /locus_tag="PK742_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQU75969.1"
                     /translation="MQVKKIAIMGAGLVGSLLSIYLKRRGYEVQVYERRPDLRSTGAA
                     GGRSINLAISDRGWKALAGAGLEEQIRKLAIPMHGRMMHSVEGGLTFQPYGQPGQFIN
                     SVSRGQLNIDLMNAAESAGVPFHFEQRVLRMDVEAGKATLENLTNQDRYELEADLIIG
                     ADGAYSMVRNSLMKTDQFNYSQLYESHCYKELSIPPAPDGGFLIDKNALHIWPRKSFM
                     LIALPNLDGSFTVTLFAPTKGPESFETITTPESLQVYFQKYFPDVLPLMPDLEADFFG
                     NPTGSLMTVKCFPWTVGNGMLIGDAAHAVVPFYGQGMNCGFEDCTVLDELLNTLDDNW
                     SKVLPAYQESRKPNADGIADLARLNFIEMRDLVADPDFLFKRKIAGKVAATHPDRFIP
                     VYSMVSFSHLPYADALKEYTRQDEVLSDVITWPHIGEKWDNEYLPRITELLQNQ"
     gene            3412..3966
                     /locus_tag="PK742_04935"
     CDS             3412..3966
                     /locus_tag="PK742_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQU75970.1"
                     /translation="MNNHEKWMQVISQVSEQVEELFGTADDLSLNIKPAPDRWSVAQV
                     LEHLILINESYIPVIRTVRNGQWKPGWLGSRPFMTRFWYRTIYSSIHPDNRKKNKTRP
                     IWEPSASSIEPGIVNRFLQQQEDFKRWITEAQDLVEQNVIIHSPAGKFITYTVGGAID
                     IMTQHELRHLIQAKETWQEVQSQS"
     gene            complement(3957..5219)
                     /gene="kynU"
                     /locus_tag="PK742_04940"
     CDS             complement(3957..5219)
                     /gene="kynU"
                     /locus_tag="PK742_04940"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HQU75971.1"
                     /translation="MNVIAGYMSLDEVRAMDATDPMASFRSHYHIPQHDGKDAVYLCG
                     NSLGLQPKKTRQYIEEELKDWETLGVEGHMHGRHPWFYYHHFLEESVARLAGASPLEV
                     VIMNTLTVNLNLLMVSFYRPTKSRYKILMEYMAFPSDQYAMEMQVRYHGFDTADAIVE
                     VKPREGEHALRTEDILQLIDEHRNELALVMMGGVNYYTGQLYDMATITKAAHDAGALA
                     GFDLAHAAGNVPLHLHDWGVDFACWCTYKYLNSGPGGASGVFIHERHAHDATLPRFAG
                     WWGNAEDTRFQMKKGFHPQPGAAGWQQSNAQILPMAAHRAAMELFDQAGMDALRTKSL
                     ALTGLLEELLQIQPGKFHIITPADPAQRGCQLSILTGAGGRQLFDRLTASGVIADWRE
                     PNVIRVAPVPMYNSFEDVYRFAELMYQD"
     gene            complement(5321..7858)
                     /locus_tag="PK742_04945"
     CDS             complement(5321..7858)
                     /locus_tag="PK742_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75972.1"
                     /translation="MKTNWLLILLTICHSVYGQTNSISISGTNYDIQEDLYGIHIQKY
                     FTNTYTSTSSTTYVGMDPELAEEGLIGLQPEIVRFPGGGSSRFMHPHPSYGSSYSYGW
                     NLVEICNYYAYVMDDYCSCNCEELAATASVDGTFDDFDELDDFFSEMGSVTDCSDAAT
                     DMENNEGGRLREVISNQNDAASPYIDQFITLILNMEDELERDVDVVYCANILTSSATE
                     IVETLNYLITSGVNVVAVELGNETYSNFNAPVFNDDMNYKGFENYWDYINGIGLSASC
                     TKYDELIAKPRNGNTDHDFIDAIRSTYSWGINIKIGVPSIGHDDFTNVFRFSEEEEEE
                     EFTPGTMPMGCPGRHGDWNAGIQSKMSETVEVSGIDYPSFDGVVIHPYYADYDLFDRM
                     EAIVCAAGGIEDDVPDCEDNEIQQLTEEYDFSMVVEELDPAFDGNLLFTTYLFLKRNM
                     ELAGVSSNDGYFEQFIKAIQDDYGFFGGEYGKELWTTEWNYANLSDVVPYAGGPKDLF
                     SIYGNTLWQGYVVFEYLIRQIQANLLSITDDDGLGFDADFYTISTIHHFLGESNVHLI
                     SNRKDNIGDYNAPVLEDDEYVRRISFLSMAMLAPIFNLDLKYTACSYGGVNKKLYLTT
                     FNKSGEYFVYYVNLNNTDQEVTISGLDSYYTPPPIPMECPTCWRQHYQLNYVGGDECY
                     LDNCYLYNTMGSSYLFDTDFNTFYSDPADIPFTIQPDINKENVILPLPEEEMSEDLSV
                     ILPKMSFGYVYIPVHTWGTLRESGYTQKLIAYPNPTSSNVQIELSDSIFGDIEVFDNL
                     GAIVLQYSPNTYETLINLDLSNLANGLYHIKIVFNDMDVEMIPIIKN"
     gene            complement(8192..8704)
                     /locus_tag="PK742_04950"
     CDS             complement(8192..8704)
                     /locus_tag="PK742_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75973.1"
                     /translation="MNDFYHFMSSGHPNAVQINRIIDSIRGRYISIFTILEGSIDRVI
                     AGYYAPTKTKRDHLLTNLLGHRFCSFELKSSVLTEILKDSGIEGYKAKLSGLAQLRNA
                     LAHRPIYAIGEFTEDPNELFYYIPSYQMNYEHNLRLESIKYELTEENFNEYSEKTLEI
                     AKMLDQFVSK"
     gene            9134..9577
                     /locus_tag="PK742_04955"
                     /pseudo
     CDS             9134..9577
                     /locus_tag="PK742_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024172.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            9719..10381
                     /locus_tag="PK742_04960"
     CDS             9719..10381
                     /locus_tag="PK742_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQU75974.1"
                     /translation="MQLRTIFYIDGFNFYYGLRTAKAKIHARWQQYYWIDLVGFCRSF
                     LDPNIHELVCVKYFTAMPLDPNKASRQTTFFNANRFVNNDLLRVIYGKYYTKTVKCNA
                     IGGCHQEFDLREEKRTDVNISVNLIEDCVFDNVDLIVLISADSDLVPPLEFIRRNYPN
                     KRIKIFFPPRRNSFDLNRFVRKKAKRLENNEGKFRSSIMDNIIGNDEISYEIPPSWVY
                     IE"
     gene            10819..10890
                     /locus_tag="PK742_04965"
     tRNA            10819..10890
                     /locus_tag="PK742_04965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10852..10854,aa:Ser,seq:gct)
     gene            complement(11283..>11748)
                     /locus_tag="PK742_04970"
     CDS             complement(11283..>11748)
                     /locus_tag="PK742_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU75975.1"
                     /translation="DDIWVLDIVLEQGSYEYKFAYDAWTGQEELTEGDACTITTDAFT
                     NRALDVSSDTVLTAVCWGSCDECVTIPEAIENVTSNLSVFPNPASEQIFISGLPASGI
                     VQLFNSLGQPVRSEQITSVSGSISVVGLSAGVYTIQVQDQDGQIQSSIILIR"
BASE COUNT         3444 a         2612 c         2470 g         3222 t
ORIGIN      
        1 gatatggccg gactgaatct gcccagttat ctgaaggggg atgggaatga aaaaagagat
       61 gctgattcag gaattcagga tacggaataa agatcactcc ttcacccact ggctatgaca
      121 cacatcttca tcgccatcgg cggtgatggt gtaggtccag aaaagggtat ctgcacctga
      181 tgacatggtg gcattaccca ggaagtacac gcctgtcttg agtgtatcca gttccagtat
      241 ctcattataa gtgccggtaa caggcagatc aaaagtggct gacaaaccga agttgctgat
      301 attggctacg gtcacttcct gcccttcgca atcgcaatcc tgaatcagac ccacataagt
      361 atagatgcct gaggtacagg tgtcagtagc ctggtagtag aggtggtggt agttcgtggt
      421 ggccagcacg atatctacat cgcgaacagc ggtctggctg ttagctgcct catcggtggc
      481 tgtatagatg acctggtaag atccgtagtg cgatacgtcc acctgcgaat ctgtgtacag
      541 catgccttcg gtattgcagg tgaggttgtc cagcaccgaa gcacccggat ccacatacgg
      601 ttcatccaga taatgaacag gctgcgggtc cggcaggagg gtaatacgcg gagcgatggt
      661 atcaggtctt tcacaggacc agatcaaagg gaggactgaa aggacaaacc agcgcatggc
      721 ttaaaggtaa aagaaaaccc cggcacaagg ccggggtcat ctctaagatt tgatctgaat
      781 cagttctgtt cgatatctac atcagaacag gtgaaggagt cagtaccatc gtcaaaggta
      841 taggagacgt tgaagtgcat agcaccgctc ataccgtcaa ccagtgaacc tgaagctgta
      901 cagaacagtg tggcatcatt atcatcagaa tcattgatgg taaccacgtc gttcaggtcg
      961 ccgctcagtt ccatgtcaaa cagggtctca ccggcatcaa aatcgcccag gttagatacc
     1021 cgtacagtag tggcgttgat cttaacgatg ttgatctcat aaggatcgat gctgccgata
     1081 cagttttcgg taacggtgaa gaaacctaca tagtcatctg cagttgcacg aacccataca
     1141 tcacgttctg cggtaccggt gttgccggct tcatccacta cggtgtaggt tacagtgtac
     1201 ttgccaacca tggcggtatt tactcctgta gcatcagtaa cgatagaagc agagatatct
     1261 ccgtcttcgt tatcggtagc aatggcgcca gcttctgtgt aagcatcgcc cagatcgatg
     1321 atctgtgggt tatcgccgtt gagtgtgacc accggggcat ccagatcatc ggtaccacaa
     1381 ccggtcataa acagtactcc ggcacccaaa aacagggctg gaactaaaat tttcattctt
     1441 ttcatgacgt taacgtttaa atttaggtgc aaaattaacc ttaggatttt ggttattctt
     1501 acttttgttg tcaaatatta ttccaaattt taaaatctta acaatgaaaa tcaggaatct
     1561 cgctatcatt ttctctctgg gcctgcttgt tgcctcatgt accgacaaaa aggcagctga
     1621 agagatcgaa aaactgaaaa tggacatggc agccgccgac agcctctgcc aggctgacaa
     1681 ggccatgttg atggactcta ttgccaacct gcacatgatg tgggaagaaa tgtcagctcc
     1741 tgccaacaag accagcagcg gcagcaccaa gtcatctact tctaccacta caaccacaac
     1801 agataccaag agcgacgaca aatctgtaaa tgatcgtggt ggttctaccc aggtagagaa
     1861 aaaggtaact gaccgcggtg gttcagatgc agaaaagacc gtcaagaagg atatcaacaa
     1921 gcgaggcgga aactgataac cctattcata tagcaaaaag cccggcctga ggccgggctt
     1981 tttttattga ttctgcaaca attcggttat cctgggcagg tactcattgt cccatttttc
     2041 cccgatatgg ggccaggtga tcacatcaga cagcacttca tcctgcctgg tgtactcctt
     2101 taatgcgtca gcatagggca gatgactgaa gcttaccata ctgtacactg gtatgaagcg
     2161 gtcaggatgt gtggctgcta cttttccggc tatctttcgt ttgaacagaa agtcagggtc
     2221 agccaccagg tcacgcattt caataaaatt caacctggcc agatctgcta taccatcggc
     2281 attgggcttg cggctttcct gataggcagg taagaccttt gaccagttgt catccagggt
     2341 attcagcaac tcatccagca cggtacagtc ttcaaacccg caattcatcc cctggccata
     2401 gaatggcact actgcatggg ctgcatcgcc aatgagcatg ccatttccca cagtccaggg
     2461 gaaacatttc acggtcataa gtgagcctgt cggattgcca aagaaatctg cttccaggtc
     2521 tggcataagg ggcaacacat ccggaaagta tttttgaaaa tatacctgca gtgattccgg
     2581 tgtagtgatc gtttcaaatg attccggtcc tttggtgggt gcaaacaggg ttaccgtaaa
     2641 actgccatcc agattgggca gggctatgag catgaagctc ttcctgggcc agatatgtaa
     2701 agcattctta tctataagga atccaccatc cggagcagga ggaatactca gttctttata
     2761 gcaatgagat tcatagagct ggctgtagtt gaactgatcg gtcttcatca ggctgttgcg
     2821 caccatggaa taggcaccat ctgcgccaat gatgagatcg gcctccaatt catagcgatc
     2881 ctggtttgta agattttcca gggttgcttt ccctgcttcc acatccatcc gcaaaacccg
     2941 ttgctcaaaa tgaaagggca ctccggcaga ttctgcggca ttcatgagat ctatgttcag
     3001 ttgcccgcgt gataccgaat tgatgaactg cccgggttga ccataaggct gaaaggtgag
     3061 cccaccttcc acactgtgca tcatcctgcc atgcatgggt atggccagtt ttctgatctg
     3121 ttcttccagc cctgcaccgg ccagtgcctt ccagccccgg tcactgatgg ccaggttgat
     3181 ggaacgtccg ccggcagcac cggtagaacg aagatcagga cgccgttcat acacctgtac
     3241 ttcataccca cggcgtttca gataaatgct gagcaaggag cctaccagac ctgctcccat
     3301 gatggcgatc tttttaacct gcatgagaca aaagtaggga atgcgttatc acttacaggt
     3361 gactttggtt ggacgcagca tccggtgatg actaaccact acctttaacc catgaacaac
     3421 cacgagaaat ggatgcaggt tatcagccag gtttctgaac aggtggaaga actgtttggt
     3481 actgccgatg acctttcact gaacataaaa cctgcaccag accgctggag tgtggcccag
     3541 gtgttggagc atctgatcct gatcaacgaa agctacattc cagtgatcag aactgttcgc
     3601 aacggtcagt ggaaacccgg ttggttggga agccgtccct tcatgacccg tttctggtat
     3661 cgcaccattt atagttccat tcatcctgat aaccggaaga agaacaagac ccgcccgatc
     3721 tgggaaccat ctgccagttc aatagaacct ggtatcgtaa accggtttct gcagcaacag
     3781 gaagacttta aaaggtggat taccgaagct caggatctgg tggaacagaa tgtcatcatt
     3841 cattctccgg caggaaaatt cattacctat accgttggag gggccattga catcatgaca
     3901 caacatgagc tgcgacatct gatccaggca aaagaaacct ggcaggaagt acagtctcag
     3961 tcctgataca tcagctctgc gaaccgatat acatcttcaa aagagttata cattggtacg
     4021 ggggcaaccc ggatcacatt gggttcacgc cagtctgcta tgacacctga tgccgtcagt
     4081 ctgtcaaaca actgtcggcc acccgcacct gtaaggatgc tcagctggca cccgcgttgt
     4141 gccggatctg caggggtaat gatgtggaac tttcccggct ggatctgcaa cagttcctcc
     4201 agcagtccgg tcagtgccag gcttttggta cgcagcgcat ccatacctgc ctgatcgaac
     4261 aattccattg cggcacgatg ggcggccatg ggcaggatct gggcattgct ctgctgccag
     4321 cctgcagcgc cgggttgcgg atggaatcct tttttcatct gaaagcgggt atcttctgca
     4381 ttaccccacc agcctgcaaa tctgggtagc gtggcgtcat gcgcatgccg ctcatgaatg
     4441 aatacgccgc ttgctccacc gggtccgctg ttgagatact tataggtgca ccaacaggca
     4501 aaatcaacac cccagtcatg caggtgcagc ggtacatttc cggcagcatg tgccagatcg
     4561 aaaccggcaa gagcacctgc atcatgcgcc gcttttgtga tggttgccat gtcatataac
     4621 tgaccggtat aatagttgac ccctcccatc ataaccaggg ccagttcatt ccggtgttca
     4681 tcgatcagct gcaggatgtc ttcggtgcgc agggcgtgtt caccctctct gggtttcact
     4741 tccacgatgg catctgccgt atcgaacccg tggtatcgca cctgcatttc catggcatac
     4801 tgatcagacg gaaaagccat gtattccatc aggatcttat agcgtgattt ggtaggacga
     4861 tagaagctta ccatcagcag attgaggttg accgtgaggg tattcatgat cactacctcc
     4921 agaggagatg ctcccgccaa tcgggcgacg gattcctcca gaaaatggtg gtaatagaac
     4981 catggatgac gcccgtgcat atgaccttcc acaccaagtg tctcccagtc tttcaattct
     5041 tcttctatat actgtcgtgt ctttttaggt tgcaatccaa gcgagttgcc acacagatac
     5101 acggcatctt ttccgtcgtg ctgaggtata tggtagtgtg atctgaagga ggccatcgga
     5161 tctgtggcat ccatggcacg cacctcatcc agactcatgt atcccgcaat tacattcatc
     5221 ccgcaaaggt aagcagcagg aaaataacga tattaaaaga gtaatttaca atttaacaaa
     5281 aataatctta ttcgctctga gaaccatatc acaagcacga tcaatttttt attataggta
     5341 tcatttcaac gtccatatca ttaaaaacta ttttaatatg atataagcca tttgccaaat
     5401 tagacaagtc taagttaatg agtgtttcat atgtatttgg gctatattga agaactattg
     5461 cgcctaaatt atcaaacact tcaatatccc caaaaatact atctgataac tcaatttgca
     5521 cattggaact tgttggattt ggatatgcta taagcttttg ggtataacca ctttctcgta
     5581 aagtgcccca ggtatgaaca ggtatataaa catatccgaa actcattttt ggtagaataa
     5641 cacttaaatc ctcagacatt tcctcctccg gcaaaggcaa gattacattc tccttattta
     5701 tgtcaggctg tatagtaaat ggtatatccg caggatctga ataaaatgtg ttgaagtctg
     5761 tatcaaacaa atatgatgat cccattgtat tatacaaata gcaattatct aaataacatt
     5821 catccccacc tacataattt aactggtaat gttgacgcca acatgttgga cattccatgg
     5881 gtataggtgg tggagtataa tatgaatcta agcctgaaat tgttacttct tgatcagtat
     5941 tattcaagtt aacataatac acaaaatatt ccccagactt attaaatgta gttaagtaaa
     6001 gctttttgtt aacgcctcca tatgaacatg cagtgtattt caaatcaaga ttgaaaatag
     6061 gtgccaacat tgccattgac aaaaatgaaa tcctcctaac atactcatca tcttcgagga
     6121 caggtgcatt ataatcacca atattatcct tacgattgga tataaggtgc acatttgatt
     6181 ctcccaaaaa atgatgtata gtcgaaatag tataaaaatc tgcatcaaaa cctagaccat
     6241 catcatctgt aatagacaat agatttgctt gtatctgcct aatcaaatat tcaaatacta
     6301 cataaccttg ccaaagagta ttaccatata tgctaaatag atcttttgga cctccagcat
     6361 atggaaccac atccgaaaga tttgcataat tccattctgt agtccaaagt tcctttccat
     6421 attcacctcc aaaaaaacca taatcatctt gaatagcttt tataaactgc tcaaaataac
     6481 catcgttact ggatacacca gccaactcca tatttctctt taaaaataag taagttgtaa
     6541 ataacaagtt tccgtcgaat gcaggatcaa gttcctctac aaccatcgaa aaatcatatt
     6601 cctcagttaa ttgctgaatt tcattatcct cacagtctgg tacatcatct tcaatacccc
     6661 cagctgcaca tacaatagcc tccattcgat caaaaaggtc atagtcagca taataagggt
     6721 gaattacgac gccatcaaag gaaggataat caatgcctga aacttcaact gtttcactca
     6781 tttttgactg tatacccgca ttccaatctc catgtcttcc agggcaaccc attggcattg
     6841 ttccgggggt aaactcttcc tcctcctctt cctcactaaa tctgaataca ttggtaaaat
     6901 catcatgtcc aatacttggc actccaattt ttatatttat accccaagaa tatgtacttc
     6961 taatggcatc aataaaatca tgatctgtgt taccatttct tggctttgca attaattcat
     7021 catacttagt gcaactcgct gacaagccaa ttccgttaat atagtcccaa taattttcga
     7081 atcctttata attcatatca tcattaaaaa ctggagcatt aaagttagaa taggtttcat
     7141 ttccaagctc aacggctact acattaaccc ccgaagtaat aagataattt aaagtttcaa
     7201 ctatttccgt ggcagatgac gtaagtatgt ttgcacagta tacaacatct acatctctct
     7261 caagctcatc ctccatattc aaaattagag taataaattg atcaatatat ggacttgcgg
     7321 catcattttg attgctaatc acttccctta acctaccgcc ttcattgttt tccatatcag
     7381 ttgctgcatc tgaacaatca gttacacttc ccatctctga aaaaaaatcg tcaagctcat
     7441 caaaatcgtc aaaagttccg tcaacactag ctgtagcagc taattcctca caattacagc
     7501 tacaataatc atccattaca tacgcataat aattacatat ctcaactaga ttccaaccgt
     7561 aagagtagct ggaaccataa gacgggtgcg gatgcataaa tctgctactt ccgcctccag
     7621 gaaatctaac aatttctggt tgaagtccaa ttagtccctc ctctgccaat tctggatcca
     7681 ttccaacata tgttgttgag cttgtgctag tatatgtatt ggtaaaatat ttttgaatat
     7741 ggattccgta gagatcctct tgaatatcat agtttgttcc actaattgat attgaatttg
     7801 tttgcccata aacagaatgg caaattgtca ataatatcaa gagccaattg gttttcatat
     7861 atactgtatt tagattttgc gaatatacta tattaatttt attcttctct tttacgaacc
     7921 tatatcatta ttgggaatag ccttacaaga tatttatata cagcatgctc ggaggttgca
     7981 accgtaacag attaagtcta tctaagaaat atttgaagaa cacctaataa tttattaact
     8041 atatacataa tgggtacatt tgttgtcctc gttctgaaca ggggggctat cagaaggttt
     8101 tctctgtggg ggagttgaaa gggaataggc atattcagct ttcagcgaga cggtattacc
     8161 aaatccaaag gcggcaaggt gataccgttt tttatttgga aacaaactgg tctagcattt
     8221 ttgctatttc taatgttttc tcgctatact cattaaagtt ttcttcagtt agctcatact
     8281 tgattgactc taaccttaaa ttgtgttcgt aattcatttg atatgatggt atatagtaaa
     8341 atagttcgtt cgggtcttct gtaaattccc ctattgcata tataggtcta tgtgcaaggg
     8401 catttcttaa ttgagctaaa ccagacagct tggctttata tccttcaatt cctgagtctt
     8461 taaggatttc tgttagaaca gatgacttta attcaaagga acagaatcgg tgtccgagaa
     8521 ggtttgtcaa aagatgatct ctttttgttt ttgttggagc ataataccct gcaattactc
     8581 tatcaattga tccttcaagt attgtgaaaa tactaatgta gcgacccctg atagagtcaa
     8641 ttattctatt gatttgcact gcatttggat ggccactact catgaagtgg taaaaatcat
     8701 tcattgtaat ttaggtattt attgggaata gatttatttg tgactaaaag cctgagccat
     8761 gactaaattt aagacattat tacagctaca aaagcacttc cacaatgagc gggtttgttt
     8821 tgagtatttg gagctgaagc gatggaatgg caagccagag tgtccgcatt gcgggagtga
     8881 acactattac cggaccaaga caaggtttaa ggacaggggc ttagacgggt atcagcagtt
     8941 tgcagctaag ctgtgacctg ggagtaactc agaaaacagc ttggttcatt ctgcaccgca
     9001 tcaggactat gttaggaaca aatgcagccg aactcctgac cgaagacgtg gaattagatg
     9061 aaacctatat aggaggaaaa gaaagcaaca agcacggcaa gttccgaaag aatacccgaa
     9121 cactgacggc cctaaatggg tgaacaagaa aacgcatgta ttgggtgcat tggagagaaa
     9181 gggagagatc aggtatttac aggtagaacg tgcattggcc aagaatatga gagagtttgt
     9241 cctcgccaac gtcaagccag gggctattct acatactgac gaatacagtg gttatcggcc
     9301 atcgcacagg ctctatcttg tggacagcgt agcacatggt cggggagagt atgtcagggg
     9361 taatgtccat atcaacacca tagagggggc gttcagcctg ttgaagaggg gtattattgg
     9421 catctaccat tatgtcagcc cgaagcacct gcagaagtac tgccatgaat ttgaatatag
     9481 gtacaacacc cgcaatgcca cagataccga acgcttcgac aaggtattgg agagagtagg
     9541 caatgcccgg ctcagaaata ttgatttaat tacttgataa ttagcaagtt aaatgcaaat
     9601 agtatgaaaa taagtgttaa atttttgcgt ttttgtctag ttggacgtta gatttgcagt
     9661 ataacctcgc taactggtaa ctcggttagt ttgccgccga catacaggcg gtttttttat
     9721 gcaactaaga actatattct atattgacgg attcaatttt tactatggct taaggacagc
     9781 caaggcgaaa atacatgccc gttggcagca gtactattgg attgatttag tggggttttg
     9841 tagatcattt ctagatccaa atattcatga attagtatgt gtgaaatact tcacagccat
     9901 gccccttgat ccaaataaag caagtaggca aacaacattc tttaatgcga acagatttgt
     9961 caataacgat ttattgaggg tgatttacgg taagtattat actaagacgg ttaaatgtaa
    10021 tgctattgga ggttgccacc aggagtttga tttaagagag gaaaaaagaa ctgatgttaa
    10081 tatttcagtt aatctaattg aagactgtgt tttcgacaat gttgatttaa ttgtactaat
    10141 ttctgcagat agtgacctag taccaccgtt agagtttata aggagaaact atcctaataa
    10201 aagaataaaa atttttttcc cacctagaag aaacagtttt gatctgaatc gatttgttcg
    10261 gaaaaaagct aaacgtttgg agaataacga gggtaagttt cgcagttcca ttatggataa
    10321 tattattgga aatgatgaaa ttagctatga aatccctcca tcatgggtat acattgaata
    10381 aatcaagtgt tggagataat ggcagaacga ccaaagaaat acgaaaaaga agctgaagat
    10441 ccacggagac tttgagcagg tcatagacgc tgcctttaac accccgcacc cagaactcac
    10501 agaagtacgc ctacaccatc cggaaaaggg agagcgcacc gtcaaggcct tagaagtggg
    10561 caaagactac atcatagtca gcgcagccgt aggcaggagg gagcagtatg aagccccaga
    10621 atatcataaa taagttgaat ttggtgaaga attaacacga cttaaattgg gccctccaac
    10681 aaacatctta agagattagg tacaaaaaat tcgataaatt tgtgtgcctt aagatgaggc
    10741 gaagatttga atagatggat cacccaacaa gcaaggggtg aaaaaaaggg gatcatttag
    10801 acccccctac tttctattct gcatgtagtt caacggatag aacaccagac tgctaatctg
    10861 gagatgtggg ttcgattctc accatgcggt cttcgcccct caataaattc ttccattaat
    10921 tccatgcggt ccacttaagg cagttttaga gtgaccccaa gtccacttag ttttacaaag
    10981 ttaactgcaa ataaactaga ttgtttcaat aatgcaagaa taattgtatt tagaacttgt
    11041 taactattgt ttcacagctc gcaatcagtt aacccccatt aactgaaatc gcctcaaatt
    11101 cagtcttagc cgaacaaatt acttaggcta ttctcaaaaa tttcttagac aaacctaatc
    11161 aggtcagcta attacctgtg taaattgctg tatattaacg ccttacaaag ctgttcccat
    11221 taggtatata gtcgccaaac aaagacggcc ttccggccgt ctttcaaaca aactgaacgt
    11281 tattaccgta tcaatataat gctgctctgt atttgaccgt cctgatcctg cacctgtatg
    11341 gtgtagaccc cggcggaaag tcctacaaca gaaatactgc cagatactga tgtaatttgc
    11401 tctgaacgga ccggctgacc cagactgtta aacagctgaa caattccgga agcaggcaaa
    11461 ccactaatga atatctgttc actggccggg ttaggaaata cagacaagtt tgacgtaaca
    11521 ttttcaatgg cctcaggtat tgtcacacat tcatcgcatg atccccagca aaccgctgtg
    11581 agtaccgtat cagaactgac gtcaagcgcc ctgttcgtga atgcatcggt agtgatggta
    11641 caggcatcgc cttcagtcag ttcttcctga cctgtccatg catcataggc aaacttgtac
    11701 tcataggagc cttgctccag cacgatatcc agcacccata tatcatca
//
LOCUS       DAOMAX010000061        11644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_721_length_11644_cov_4.270256, whole genome shotgun sequence.
ACCESSION   DAOMAX010000061 DAOMAX010000000
VERSION     DAOMAX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11644
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_721_length_11644_cov_4.270256"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PK742_04975"
     CDS             complement(<1..693)
                     /locus_tag="PK742_04975"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQU75976.1"
                     /translation="MRMRFLKYLAGYTIPVVVWVSISQDGLWSFTAFAYAFVLISILE
                     LFLPDDPSNLEATEEELAKKDRIYDWMLYLNVPIQFGLLWFFLDTVSTGTFTWWELVG
                     KVHAMGLSCGVTGINVAHELGHRVKPYERVMAKLLLMSSLYMHFIIEHNRGHHKRVST
                     AEDPASARKGEGLYSFYGRTLIMSIGSAWQLEKKRLLHKGGKIWSLQNEMLRFFLFQC
                     LFLVAIGWFFGLF"
     gene            721..1398
                     /locus_tag="PK742_04980"
     CDS             721..1398
                     /locus_tag="PK742_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQU75977.1"
                     /translation="MSGTYSALLLFHHVISILYMTGFMTPIQFSDLDLSRAVDFDTYL
                     QKMQEWVAQGATSGPVQSESLIQYTKLNLARMERQLKTIQLLPEQEVLEGRLRPMEWV
                     VITESWCGDAAQTLPLMHITAKFLHIPMHLVLRDEHPELIERYHTNGAHAIPKLVMFD
                     RETGEELAVWGPRPEPAQTMVLSAKQAGIPHDDYLRDLHTWYGRDKNLTLQTELVELL
                     MQFRRTA"
     gene            1395..2126
                     /locus_tag="PK742_04985"
     CDS             1395..2126
                     /locus_tag="PK742_04985"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQU75978.1"
                     /translation="MISYRNVNPNKRFSASREEENSLLFTSFFMPLVAIALMWLVKAF
                     EHVSGYDLAVHGIFPRTLKGLQGVLFSPFLHGDWQHLVSNSLPFLILGFLMLYSYRKV
                     AFKSFVFIWFASGFMVWLTGRPSYHIGASNLVYGFAFFLFFSGIFRRDIQSIAMALLV
                     TFIYGSMVWGIFPFRWEISWEGHLMGGISGAVMAWYYRKVDLPEPVELEDEEEDDDAF
                     FDDDDDSPIRYLYKEDEDRAHPDGR"
     gene            2095..2748
                     /gene="mnmD"
                     /locus_tag="PK742_04990"
     CDS             2095..2748
                     /gene="mnmD"
                     /locus_tag="PK742_04990"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HQU75979.1"
                     /translation="MKTERIRTGDDSYTLRVPELDEHYHSIHGARAESMHVFIDAGLK
                     AVKNKPSIHLLEVGLGTGLNCLLTAAHQQPETTIRYTALEPFPLEKEILDSIAPENEP
                     ERGRFWQIHHTPADGDAQITPGFLLHRSMQKLETLALHDPVDLVYFDAFAPRVQPELW
                     TTEVFRLLYDLMSDNSILVTYCAKGDVRRAMQQAGFQVERLPGPPYKREMLRARKNS"
     gene            2729..3769
                     /locus_tag="PK742_04995"
     CDS             2729..3769
                     /locus_tag="PK742_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQU75980.1"
                     /translation="MPEKTADILIIGGGIAGLSMAAALDMKGMPFVLCASPSTEPASA
                     VAAGLINPVTGRRYTLQTRYEQLMASAKEVYAHLAGVSGKSILEAINITRLHRSAEAR
                     EAFDALTDIPNAYLNTNAPLHAGQVHTEYGGMQVLNGFRVDVSALSGNLAMLHFQHGT
                     VQHFVLDFPISFSQERYHTALGSFHTIIDCRGYQTAFDQSWQFIPFRQNKGEVLRVKI
                     TDPPSAGVYIKDCILVPLQDGTWWAGAHNSWTDHSPFPTTAGKESLENALNKLLSSSY
                     KILDHQAAFRPAIRDRAPVVGRHPHLQRYYLLNGLGTKGYSLAPYHALMLADHILNEK
                     PIDPKVDVARFY"
     gene            3793..6228
                     /locus_tag="PK742_05000"
     CDS             3793..6228
                     /locus_tag="PK742_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQU75981.1"
                     /translation="MRLQKLLLTVLITPLISVAQVHTEQTYVVDRFGQPRDHNVDMEH
                     LFLDIAIDPYQGMVAGVANYVFHPIQQQVDTLFLDGPGIAVNAVKVDGNSARFRVDSA
                     GVTIFFSPSLSWGSTHELNIEYTAHPKKGIYFVGWNEDTKDIATDPDRIRNQVWTQGQ
                     GTDNRFWIPSFDDVNDKLLTSLRITFDKNYRVISNGNLTDERNNSDGKTKTWVYEMAH
                     PHANYLIMLAIGDYAYRDYTSKNGIVSRQYYYPDLEDRVEPTYAYSAEMMDWISEETG
                     LNFPWPTYANVPVQEFLYGAMENTTATIFTDALFQDAGGQNDRSYVAVNAHELAHQWF
                     GDYVTEWGNRDHWLQESFATHYSKKFRQSVYGEDEFQWIRRGEMRAAIAADNRDTYPL
                     AHSLAGSSRHYPKGSFVLDMMRYVLGNEQYRKVINDYLLAHPYENVRSEDLEIQCMKS
                     LGINMWWFFDQWVYRSGYPVYQVNTTFTSDTTTITVVQKQDSTATQHLFRMPVVFQVH
                     YKDGGFDQETRWVEDSTTVVQIPNILKREVAFVLFDPGPQNISQVEFEKSFDAYKMQA
                     YNAPLMIDRYDAVVAMQKVAIDDKRDILIDLYDKQNYHAIREEIIAQLANDDHKKSIA
                     LLKRALKDTDINVRRAVVTNLKTLPKKMEKDIAPMLDDSDYTNVELALRFLCEQDKEH
                     AEKYLEHCADKYGAAKNIRIAWLEYSYANGNIIAAPAELAKYASNLYEFRTRGNAIQA
                     INRLNICDAAIAASLVEATQSFNRRLAGPTTRVLKGFYENKLYTSIIDKAIDDAIQAG
                     GDKEKLEALRKKD"
     gene            complement(6229..6666)
                     /locus_tag="PK742_05005"
     CDS             complement(6229..6666)
                     /locus_tag="PK742_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU75982.1"
                     /translation="MKLQEVIVQHGSPEYDLVVGLRYNILRKPLGMQFTTEQLAAESK
                     NHHLALFHGETPVACLMLLPVTENRSMQMKQVAVDDTLQGQGIGARLIAFAERFARAH
                     GAHTMFCHARQNAVPFYLRQGYVIDKEPFEEIGIPHRYMEKKI"
     gene            complement(6663..8000)
                     /locus_tag="PK742_05010"
     CDS             complement(6663..8000)
                     /locus_tag="PK742_05010"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQU75983.1"
                     /translation="MQRIFVIGAGKSSTVLIRYLLDHAAANDWFVTVGDMSAELANQK
                     VAGHPCGEGVTFNVLDDEQRVSSIRQSDLVISLLPANMHGPVAADCVHYGRNFISASY
                     VSPEMSALHEAAVDRGVLLLNECGLDPGIDHMSAMRMIHDAQARGGVIHSFESYAGGL
                     IAPESDNNPWKYKFTWNPRNIVLSGQGVARFYWKDQLKLIPYNKLFQRYDLLEVPGYG
                     TFEGYPNRDSTKYKSLYGLDGTHTLVRGTLRKKGYCDAWNALVALGITDESYEVDLPQ
                     GTTWAQFIDNYLPFDPELSLKEKYIRYFQLEQLPEVVEKLEWLGLFSDRKLDRFQGSP
                     ARIFQDLLEDKWKLDTGDKDMIVMLHKMEVEMDQKMWEVQMSLAVCGDDEMITAMAKT
                     VGLPLAITAKNVLAGKIQRTGVVIPVTPDIYMPILHELEQDFGISFIHEGKEI"
     gene            8066..8536
                     /locus_tag="PK742_05015"
     CDS             8066..8536
                     /locus_tag="PK742_05015"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQU75984.1"
                     /translation="MEVIRHTTELRHYSSVNELSKPHAALLAEAARVAGQAYAPYSNY
                     QVGCAIELANGEIICGSNQENASFPAGTCAERSALFYAGSRYPGVAIVRVAVTTLFPA
                     ADPVAPCGICRQALLEYENRQDHPIELLMSHPGGRVYVSDSVANILPVFFRYKK"
     gene            complement(8648..10099)
                     /locus_tag="PK742_05020"
     CDS             complement(8648..10099)
                     /locus_tag="PK742_05020"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HQU75985.1"
                     /translation="MQLWFIEGTPPRDPISVTNLWTGYKGYNTDEAFDDNTPAITMNL
                     PADASEFRIKSRVSGHGFGGTANCAEFCAKEHYFKVNDDVVWTREVWRDNCDLNPVYP
                     QGGTWVYDRANWCPGAEVWTYDFELTPFVTPGSTYTFDYAAEDYNWNGSGSFPYYQTE
                     VQLVAYGPYNFDLDAAVTDILAPSGDDMWGRMNPICNNPIIRIQNTGASTLTSLKVEF
                     GLKDAPYSTYNWSGELQPMEWEEVTLPDYVYQPADQFVVTISEPNGGADEYELNNTMT
                     SEVAFPVSYPADLIIEFKANNKPNENDWFVYDANGNELYSRTTFTAGTIHKDTIHLED
                     GCYEFYLWDWDEDGISWWANSDGTGYLRIKDANTGTILKSFEPDFGGLIYQQFTVGTY
                     VDIDEPLDAEDAFEVYPNPAADHLYLKIRLSGRREAEVFIYDYAGNLIHTEKPGRIQD
                     ETLHMDVSQYASGMYVAVLKAGNTWKKVPFMVE"
     gene            complement(10733..10993)
                     /locus_tag="PK742_05025"
                     /pseudo
     CDS             complement(10733..10993)
                     /locus_tag="PK742_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11015..11368)
                     /locus_tag="PK742_05030"
     CDS             complement(11015..11368)
                     /locus_tag="PK742_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75986.1"
                     /translation="MAFTAAYSWDGTSDILVEVTYANEAGADDTWLRASDAGANRSIY
                     ASSSDRYLDLWNPVYAAVDGTPYNSLDQQITVSFWTYGDPVYQPQSGTTFEIRDDNAY
                     RVVNVHGPWGDGVFY"
     gene            complement(11328..11552)
                     /locus_tag="PK742_05035"
     CDS             complement(11328..11552)
                     /locus_tag="PK742_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75987.1"
                     /translation="MNAGDITGMQVYINEAGSLAQDLIIRMAVAPADTFNSHFYTADW
                     TTVYHNDVLPFFTRLGQCGFYCCLQLGWHF"
BASE COUNT         2953 a         3006 c         2846 g         2839 t
ORIGIN      
        1 aaacaagccg aaaaaccagc caatggccac cagaaacaag cactgaaaca ggaaaaaacg
       61 aagcatttca ttctgcagag accagatctt acccccttta tgtagcaacc gttttttctc
      121 cagctgccat gccgatccga tactcatgat cagggtgcgt ccgtaaaagc tgtacaaccc
      181 ctctcctttt ctggcagaag caggatcttc ggcagtactg acccgcttat ggtgacctct
      241 gttgtgttca atgatgaagt gcatatacaa ggaactcatc agcagcagtt tggccattac
      301 cctttcataa ggtttcaccc ggtgccccaa ttcatgggcc acattgatac cggtaacgcc
      361 gcagcttagt cccatggcat gaaccttccc caccagctcc caccaggtga acgttcccgt
      421 ggaaacagta tcaagaaaga accaaagcag tccgaactgt atgggtacat tgagatacag
      481 catccagtca tagatccggt ctttttttgc cagctcttcc tccgtcgcct ccaggttaga
      541 cggatcgtct ggcaggaaca actccagaat gctgatcaga acgaaagcgt aggcaaatgc
      601 cgtgaaagac cacagtccgt cctgcgaaat actgacccag accaccactg gaatggtata
      661 gcctgccagg tatttcagaa accgcatgcg catgatgcaa atctaaataa tggaaagcct
      721 atgtcaggaa cctacagcgc ccttctgttg tttcatcatg taataagcat cctgtacatg
      781 accggtttta tgacacctat tcagtttagt gacctcgacc ttagccgtgc cgttgatttt
      841 gatacctacc tccaaaaaat gcaggagtgg gtagctcaag gcgctacaag cggaccggtg
      901 cagagtgagt ctcttatcca gtacaccaaa cttaacctgg ccagaatgga acgacagttg
      961 aaaactattc agctgttgcc tgagcaggaa gtgctggaag gaagacttcg ccccatggaa
     1021 tgggtggtca ttactgaatc ctggtgtggt gatgccgccc agaccctgcc actcatgcat
     1081 attaccgcta agtttctgca tattcccatg cacctggtgc ttcgcgatga acatcccgaa
     1141 ctcatagaac gataccatac caacggcgca catgccatcc cgaaactcgt gatgttcgac
     1201 cgggagaccg gagaagaact ggccgtgtgg ggtccgcgac cggaacctgc gcaaactatg
     1261 gtgctttcag ctaagcaggc aggcattccg cacgacgact atctccggga tctgcataca
     1321 tggtacggcc gcgacaaaaa tcttaccttg cagactgagt tggtggaact gctcatgcaa
     1381 ttccgtcgta cagcatgata tcctaccgca acgtcaatcc caacaagcgt ttttccgcaa
     1441 gcagagaaga agagaacagc ctgctcttta ccagtttctt catgcctctt gtggccattg
     1501 ctctgatgtg gctcgtcaaa gcttttgaac atgttagcgg atatgacctg gctgtgcatg
     1561 gcatctttcc ccgaacactc aaaggacttc agggtgtgtt gttcagtccg tttttacacg
     1621 gcgactggca gcatctggta tccaattccc tgcccttcct catcctgggt tttctgatgc
     1681 tctacagtta tcgcaaggtg gcattcaaaa gttttgtgtt catctggttc gcatctggtt
     1741 tcatggtctg gctgaccggg agaccttcct atcatattgg tgccagtaac ctggtgtatg
     1801 gcttcgcttt cttcctcttc ttcagcggca tctttcgtcg cgatatacaa agcattgcca
     1861 tggccctgct cgtcaccttt atctatggca gcatggtctg gggcatcttc ccgtttcgct
     1921 gggagatcag ctgggaaggt cacctcatgg gtggcatttc cggagcggta atggcctggt
     1981 attacagaaa ggttgacctg ccggagccgg tagagctgga agacgaggaa gaggacgatg
     2041 atgcgttctt tgacgacgac gatgacagcc ctatccggta cctttacaaa gaagatgaag
     2101 accgagcgca tccggacggg cgatgattcc tataccttac gggttcctga actggacgaa
     2161 cactaccact ccatccatgg tgcacgggcc gagtccatgc atgtgttcat cgatgccgga
     2221 ttgaaggcag tgaagaacaa gccatccata catttactgg aagtgggtct gggtacagga
     2281 ttaaactgcc tgctcaccgc agcacatcag caaccggaaa ctaccatcag atacacagca
     2341 ctggaaccat tcccgctgga aaaagaaata ctggattcca ttgctcctga aaatgaaccg
     2401 gagcggggaa gattctggca gatacaccat accccggccg atggtgatgc acagatcaca
     2461 ccgggatttc tgcttcatcg ctccatgcaa aaactggaaa ctctggcctt gcatgatccg
     2521 gtcgatctcg tgtatttcga tgctttcgca cccagggtgc agccggaact gtggacgacg
     2581 gaggtgttcc gtttgctgta tgacctgatg tcagataaca gcatactggt gacctactgt
     2641 gccaaaggcg atgtaagacg tgccatgcaa caggcaggat ttcaggtgga gagacttccg
     2701 ggtccgcctt ataaaagaga gatgcttcgt gcccgaaaaa acagctgata tactcatcat
     2761 tggtggaggc attgcagggc tttccatggc tgctgcactg gatatgaaag gtatgccctt
     2821 tgttttatgc gcatcacctt ccacagagcc cgcttctgct gtagctgccg gtctgatcaa
     2881 tccggttacc ggcagaagat atactttgca aacacgatat gagcagctta tggcatcagc
     2941 aaaagaagtg tatgcgcatc ttgctggtgt cagtggtaaa agtattctgg aggcgatcaa
     3001 catcacacgt ttgcacagat ctgcagaagc cagggaagca tttgatgcgc ttaccgatat
     3061 tccgaatgca tacctgaata ccaatgctcc tttacatgca gggcaggtgc atactgaata
     3121 cggcggcatg caggtactga acgggtttcg tgtggatgtg agcgccttat ccggcaacct
     3181 ggccatgctg cattttcaac atggaacagt gcaacatttc gtcctggatt ttcccatttc
     3241 attttcgcag gaacgctatc atacagccct cggatcattt cacaccatca ttgattgcag
     3301 agggtatcag accgcctttg atcaaagctg gcaattcatt ccgttcagac agaacaaggg
     3361 cgaggtgctt cgcgtgaaga tcacagatcc accgtcggcc ggtgtatata tcaaagattg
     3421 cattcttgtt cctttgcagg acggaacatg gtgggccggg gcgcacaact catggactga
     3481 tcattcaccc ttccccacaa ctgccggaaa agaatcgctc gaaaacgcat tgaacaaact
     3541 gctgtcatct tcctataaga tcctggatca ccaggcagct ttcagaccgg ctatccgcga
     3601 cagagcaccg gtagtgggca gacatccgca tctacaacga tactatctgc tgaacggact
     3661 gggcaccaaa ggatactcac tggcacctta tcatgcactc atgctggccg atcatattct
     3721 gaacgaaaaa cccatcgacc cgaaagtgga tgtcgccaga ttttattaat tatgagcacc
     3781 aaaattcaaa ccatgcgctt gcaaaaactt ctgcttactg tcctgatcac accacttatt
     3841 tctgtggcac aggtacatac cgaacagacc tatgtggttg atcgtttcgg acaaccgcgt
     3901 gaccataatg tggacatgga acacctcttt ctggatatcg ccatagatcc ttaccagggt
     3961 atggtagccg gggtggccaa ctatgtattc catccgatac agcagcaggt agatacactt
     4021 tttctggatg gacccggcat cgctgtcaat gcagtaaaag tggatggcaa cagcgcacgt
     4081 ttccgtgtgg actctgcagg tgtgaccatc ttcttctctc cttcgctgtc ttggggcagc
     4141 acccacgaac tgaatattga atacacggca catccgaaga agggcatcta ttttgtgggt
     4201 tggaatgaag acaccaaaga cattgcaaca gatccggatc gcatacgcaa ccaggtatgg
     4261 acccagggac agggaacgga taacagattc tggataccct cttttgatga tgtgaatgat
     4321 aagcttctga cctcgctgcg catcaccttt gataagaact accgggtaat cagcaacggc
     4381 aacctgaccg acgaacgcaa caacagcgac ggaaagacca agacctgggt gtatgaaatg
     4441 gcgcatccac atgccaatta tctgatcatg ctggctatag gcgactatgc ataccgcgat
     4501 tacacctcta agaacggcat cgtttcccgt cagtattatt atcccgatct ggaagaccgg
     4561 gtggaaccca cttatgccta ttcagcagag atgatggact ggatctctga agagaccgga
     4621 ctgaactttc cgtggccaac ctatgccaat gtaccggtgc aggaatttct ctatggtgcc
     4681 atggaaaaca ctaccgctac gatcttcaca gatgccctgt tccaggatgc aggcgggcag
     4741 aacgaccgca gctatgtggc agtaaacgca catgagctgg cccatcagtg gtttggagat
     4801 tatgtgacgg agtggggaaa ccgtgaccat tggttgcagg aaagttttgc tacacactac
     4861 agcaagaagt tcagacaatc cgtttacggt gaggatgaat ttcaatggat acgcagagga
     4921 gaaatgcggg cagccatagc agctgacaac agagatacct atccgctggc ccattcactg
     4981 gcaggctctt ccagacatta tcccaaagga agttttgtgc tggacatgat gcgttatgtg
     5041 ctgggtaatg aacaatacag aaaagtgatc aacgactatt tactggcgca tccatacgaa
     5101 aacgtgcgct ctgaagatct ggagatacag tgcatgaaaa gcctgggcat caacatgtgg
     5161 tggttctttg accagtgggt ctatcgaagc ggttaccccg tctatcaggt gaacaccaca
     5221 tttacttcag ataccaccac cattacggtc gtgcagaaac aagacagcac agccacacag
     5281 catctgttca gaatgccggt ggtatttcag gtgcactaca aggatggtgg atttgatcag
     5341 gaaacacgct gggtggaaga cagcaccacg gtggtgcaga tcccgaacat cctgaagcgg
     5401 gaagtggcat tcgttctctt cgatccgggc ccccagaaca tcagccaggt ggagtttgag
     5461 aagtcattcg atgcttacaa aatgcaggca tacaatgcgc cgctcatgat agaccggtat
     5521 gacgccgtag tggctatgca gaaggttgcg atcgatgata aacgagacat cctcatcgat
     5581 ctgtatgaca agcagaacta ccacgccatc cgggaggaga tcattgcaca gcttgccaat
     5641 gacgatcaca agaagagcat cgcactgttg aagagggctc tgaaagatac tgatatcaat
     5701 gttcgtcgtg cggtcgtgac caatttgaaa acactgccaa aaaaaatgga aaaggatatc
     5761 gctcccatgc tcgacgacag tgactacacc aatgtggaac tggcattgcg attcctttgt
     5821 gagcaggaca aggaacatgc agaaaaatac ctggagcatt gtgcagataa atacggagct
     5881 gccaaaaaca tccgcattgc ctggctggaa tattcttatg ccaacggaaa tatcattgca
     5941 gcacctgcag aactggccaa atatgccagc aacctgtatg agttcagaac aagaggcaat
     6001 gccatccagg ccatcaacag actgaacatc tgcgatgcag ctattgcggc cagtctggtg
     6061 gaagccaccc agtcgttcaa tcgaaggctg gccgggccca ccacccgggt actgaagggt
     6121 ttttatgaga acaaactata cacgagcatc attgacaagg ctattgatga tgctattcag
     6181 gccggcggcg acaaagaaaa actggaagca ctcaggaaga aagactgatc agatcttctt
     6241 ctccatgtag cgatggggaa taccgatctc ttcaaagggt tccttgtcga tcacataccc
     6301 ttgtctgaga tagaacggaa cagcattttg gcgggcatga cagaacatgg tgtgcgcacc
     6361 atgtgcacgg gcaaatcgct cagcgaaagc gatcagcctg gcccctattc cctgaccttg
     6421 cagtgtgtca tctacagcca cctgtttcat ctgcatgctt ctgttttcgg ttacaggcag
     6481 cagcatcaaa caggctaccg gagtttctcc atggaataat gccaggtgat gattcttact
     6541 ttctgctgca agttgttctg ttgtgaactg catgcctagt ggcttgcgca gaatgttgta
     6601 acgcagacca actaccaggt cgtattccgg tgagccgtgc tgtacgatca cttcctgcag
     6661 tttcatatct cttttccttc gtgtatgaag ctgatgccaa aatcctgttc cagttcatgc
     6721 agaatgggca tatatatatc gggtgtaacc ggtatgacca ctcctgttct ttggatcttt
     6781 cctgcaagca cattcttcgc tgtgatggcc agaggcaggc ccacggtctt ggccatggca
     6841 gtgatcattt catcgtcacc acaaacagca agactcatct ggacctccca catcttctga
     6901 tccatctcca cctccatctt atgcagcatg acgatcatat ccttatcacc agtatccagt
     6961 ttccatttat cttccaacag atcctggaat atcctggcag gacttccctg aaaacgatcc
     7021 agctttctgt cggaaaacaa gcccagccac tccagtttct ctaccacttc aggcagttgt
     7081 tccagttgga aatatcggat atatttttcc tttaatgaca attccggatc aaaaggcaga
     7141 tagttgtcaa tgaactgcgc ccaggtggtt ccttgaggaa gatccacttc atagctttca
     7201 tcggtgatac ccagtgccac cagtgcgttc caggcatcac aatatccctt cttccggagg
     7261 gtaccccgaa ccagagtgtg tgtgccgtcc agcccgtaca ggcttttgta tttggtagaa
     7321 tcacgattgg ggtagccttc aaatgtaccg tatcccggca cttccagcag atcataacgc
     7381 tgaaatagtt tgttgtaagg gatgagtttc agctggtcct tccagtagaa cctggccaca
     7441 ccctgtccgg acagcacgat attcctgggg ttccaggtga acttgtattt ccagggattg
     7501 ttgtcgcttt ccggagcgat gagtccgccg gcataagatt caaatgaatg gatgacccct
     7561 ccacgtgcct gcgcatcgtg aatcatgcgc atggcactca tatgatcgat gccgggatcc
     7621 agaccacatt cattcaaaag cagcacacct cgatctaccg ctgcttcatg caaggccgac
     7681 atctccgggc tgacgtagct ggctgaaatg aaatttctgc catagtgcac gcaatcagct
     7741 gcaaccggcc catgcatgtt tgcaggtaac aatgatatga ccagatcaga ctgcctgata
     7801 ctgctaaccc gctgttcatc atccagtaca ttgaaggtga ccccttctcc acatggatgt
     7861 cctgccactt tctggttggc aagttcggcc gacatatcgc ccacagtaac gaaccagtcg
     7921 ttggcggcag cgtgatcaag gaggtatcgg atgagaacgg tactggactt gccggcaccg
     7981 atgacgaaaa tccgttgcat gattgtggtt tcgggggcga agttagtact ttagggcgtg
     8041 ggcaattcaa aagccctgtg gaaatatgga agtgatcagg catactaccg aactccgcca
     8101 ttacagttct gtaaatgaat taagcaagcc gcatgctgcg ctcctggctg aggctgcacg
     8161 ggtggcaggt caggcctatg ccccctactc caactaccag gtgggctgcg ccattgaact
     8221 ggccaacgga gagatcatct gtggcagtaa ccaggagaat gcttctttcc cggccggcac
     8281 ctgtgctgaa aggtctgccc tgttctatgc cggctccaga tatccgggtg ttgctattgt
     8341 ccgggtagcg gtgaccaccc tgttccctgc cgcggaccct gtggcaccct gtggcatctg
     8401 ccgccaggct ttgctggaat atgagaaccg acaggatcat ccgatagaac tgctgatgtc
     8461 gcaccccggc ggaagggtgt acgtatctga ttcggtagcc aacatcctgc cggtgttctt
     8521 ccggtataag aaatagggtg ggatggggaa atgtgaaaat gagagaatgt ggaaatggga
     8581 aaacaggatt tttttgttac ctgaagaccc catttctact tgcgtatttt cacattcaca
     8641 cattttctca ttcaaccata aacggaacct tcttccaggt gtttcccgct ttcagcaccg
     8701 ccacatacat gcctgaagca tactggctga catccatgtg aagggtttcg tcctggatgc
     8761 gtccgggttt ttcagtgtgg atcaggtttc ctgcataatc atagatgaat acctcggctt
     8821 ctcttcttcc gctcagtctg atcttcagat acaggtgatc ggcagcgggg ttcggatata
     8881 cttcgaatgc atcctcagca tcaagaggtt cgtctatatc cacataggta cctaccgtga
     8941 actgctggta gatcagacca ccaaaatctg gttcaaagct cttcaggata gtgcccgtat
     9001 tagcatcctt gattcggaga tagccggttc catcgctgtt ggcccaccag gagataccat
     9061 cttcatccca atcccacaga tagaattcat agcatccgtc ttccaggtga atagtatcct
     9121 tatggatggt accggcagtg aaagtagttc ggctgtatag ttcgttgcca ttggcatcat
     9181 atacaaacca gtcattttca ttcggtttgt tgttggcctt gaattcaatg atcaggtcag
     9241 caggatagga aaccgggaat gccacttctg atgtcatggt attgttcagt tcatactcat
     9301 cggcaccgcc attgggttcg gagatggtca ccacgaactg atctgcaggc tgatacacat
     9361 aatccggcag ggtcacctcc tcccactcca tcggctgcag ttctccactc caattatagg
     9421 tgctgtaagg tgcatccttc agtccgaatt cgaccttcag agaggtgagc gttgaagcgc
     9481 ctgtattctg aatacgaata atgggattgt tacaaatggg attcatgcgt ccccacatgt
     9541 catcaccgga tggtgcgaga atatctgtca ccgcagcatc cagatcaaaa ttgtaagggc
     9601 cgtaagctac cagctgtact tctgtctgat aatatggaaa agatcctgat ccgttccagt
     9661 tgtaatcttc tgctgcgtaa tcaaaggtgt aagtacttcc tggtgttaca aaaggcgtca
     9721 gttcaaaatc ataggtccac acttctgctc cagggcacca gttggcacgg tcatataccc
     9781 aggtaccacc ttgcggataa accggattga gatcacagtt gtcacgccat acctctctgg
     9841 tccagaccac gtcgtcgttc actttgaaat agtgttcctt ggcacagaat tctgcacagt
     9901 tggcggtacc tccaaaacca tgtccgctta ccctggactt gatgcggaat tcactggcat
     9961 ctgcaggaag gttcatggtg atggcaggag tattgtcatc aaatgcttca tcggtattgt
    10021 atcccttgta tccggtccac aggttggtca cggagatcgg atcgcgggga ggcgtaccct
    10081 caatgaacca gagctgcatg tcgagcaatt cctgccagtt accagctgcc agacgaacgc
    10141 tgtcatgcag caacggccga tagtcactca catcaaaagt ccatgtgaat ccgtcgccca
    10201 gatcgagtcc aatgccatac ggcgtgatat agcgacctag ttcatagcgg ttcactactt
    10261 caaacggggc agaataatat tcatagtatt ctactgcgat attatcggtt gcaggaacca
    10321 ggacagaatc tgcaaccact ccatattcgt catagaggta ggtatagaca tcacccggcc
    10381 ataccagaat ggtatcgagg ccactggtgg gatcggccgg atcgaaggtg accagcgtga
    10441 ccggatccat cagcacggta tcgatcacca gaaccgagtc cagatgactg gtgaataccc
    10501 cctgttccca gatgacatcc ggacgcaggt tgctcatggt ggtatagcga aagagtgttg
    10561 ccggatcata tggtctggac accgctccga tcagtgcgag gtcagcgttg ctggaagaag
    10621 cgtcagactc ataaatgcca tcgccatcgt tgaaggagta atacaggatg agatcatcgg
    10681 cattcgggtg ggcttcatcc aggtccttat acaggtattc ctgaatggtg gcttcgtcca
    10741 gtacggcatc ccacatggca aattcatcca tccatccgta atatgactga gatcctgccc
    10801 agaggccttt cccgatgcgc atttctgttc cacctgccat gctgcgggtc ttatctgtac
    10861 cactgtgcca caacactccg ttcttgtaga tcttcatatc gccggtggag gcatctttgg
    10921 taaaagccca atgcgtccag ttgccttcgt aatcagctac ggcagcggcc ttgttaatgc
    10981 ggtcatagct tccgcctccg tcattgccgg catctcagta aaacacaccg tcgccccagg
    11041 gtccgtgcac attcacaacc ctgtaggcat tatcgtcgcg gatctcaaaa gtggttccgc
    11101 tttgtggctg gtaaaccgga tcgccgtagg tccagaagga tacggtgatc tgctgatcca
    11161 gactgttata aggagtacca tccaccgcag catatacagg attccataga tcgaggtagc
    11221 gatcgctgga agatgcatat atacttctgt tggcacccgc atcactggcc ctcagccagg
    11281 tatcatctgc tccggcttca ttggcgtagg taacctccac caatatatca gaagtgccat
    11341 cccagctgta agcagcagta aaagccacat tggcccagcc gggtaaaaaa tggcaggaca
    11401 tcgttatgat ataccgtggt ccagtcggca gtataaaaat gactgttgaa ggtatctgcc
    11461 ggagctaccg ccatgcgtat gatcagatcc tgtgccaggc ttcctgcttc attgatatac
    11521 acctgcatac cggtgatatc accagcattc atacctgctg ccagcagttc atcggcggtc
    11581 cagatatact gggtgcgacc aagcggctag gaggtggaga gcggattgct gacagatgct
    11641 gctc
//
LOCUS       DAOMAX010000062        11371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_754_length_11371_cov_4.147490, whole genome shotgun sequence.
ACCESSION   DAOMAX010000062 DAOMAX010000000
VERSION     DAOMAX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_754_length_11371_cov_4.147490"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PK742_05040"
     CDS             complement(<1..290)
                     /locus_tag="PK742_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQU75988.1"
                     /translation="MMDPARILYEDNHLIAVNKLPGEISQGDKTGDIPIGELVSEYLK
                     VKYQKPGNVYLGVLHRLDRPASGVLLFAKTGKAAERMSRIIAEREVEKTYFV"
     gene            complement(287..646)
                     /locus_tag="PK742_05045"
     CDS             complement(287..646)
                     /locus_tag="PK742_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQU75989.1"
                     /translation="MLHLESFREYCLAKPGVTEGLPFGPDVLVMKVGGKMFALAPLNT
                     PEMRMNLKCDPEDALLLRDKYPMHVLPGYHMNKMNWNTVIVDGSIPDKLLLEWIDHSY
                     DLILASLPAKVRKTILP"
     gene            complement(706..2808)
                     /locus_tag="PK742_05050"
     CDS             complement(706..2808)
                     /locus_tag="PK742_05050"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQU75990.1"
                     /translation="MKRTVLLLLSVIITSGLYAQSAEQCGTPDVDHRLQEENAGYTAR
                     RAYVEDFTQKYINKHLASGTRADVITIPVVVHVVYENDLENISDAQVLSQIDVLNEDL
                     RRLNADAADTPAEFDAVAADFEIEFCMATVDPDGNPTTGIVRIPTTSSSFGISDDVKF
                     TASGGDDAWPADEYMNMWSCDLGSGLLGYAQFPGGPDETDGVVITYSSFGREGYVIAP
                     YDLGRTGTHEVGHWLNLYHIWGDAAGCGTDDSVDDTPVQDVQTFGCPTYPLADACSES
                     IQFQNYMDYTDDACYNMFTEGQKDRARAIFEAGGARFALGNSLACVSYNFDAEALEIV
                     SPVGNYCFSTFSPIVTIRNTGLETMTSVDIQYSIDGASPLTYFWTGSLETYETEDVTL
                     PSISLGDGAHSIVVTVVDPNGQPDENSSNDEASSDFFINTFGLTLPLIQGFEVDPFPY
                     AGYTVHNPDGSFTWERTTAAAKTGSASVFMNHFENTAIGAVDELELPAYNMTGIPAAH
                     LDFDVAYANYTPGGEYSDSLEVLVSTDCGVNWTRIYYKAWPDLNTAPATGAAFIPAND
                     QWRLESLSLTPYIGDKLVVKFRTVSSWENNLYLDNINIASGTVAVNDLSDNYWFEVYP
                     NPADNWVNISYNFASGQLLHVDIIDMSGRIVFSDDLRNTPGTISQTSLPVMGLPSGMY
                     TIRINDDAHTAQRSMLKN"
     gene            3075..4379
                     /locus_tag="PK742_05055"
     CDS             3075..4379
                     /locus_tag="PK742_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQU75991.1"
                     /translation="MITDFLIIFLLICLNGLFAMSEIAMISLRKSRLETAARKGDKRA
                     AAALELHNQPGRFLSTVQIGITLISILTGIYSGDTLKEPIIGWLSQWDAVAVYADSLA
                     VTIVVIFITGLSLLVGELLPKRIGLSYSESIAKIMAAPMTMLSKVTAPFSWVLTSATN
                     GLIRLMRLKHSNDDRVTEEEIKAMIQEGTDSGEIEEIEQDIVERVFHLGDRRIASLMT
                     HRSDIVWLDINSGPEELKHTISTEVHSVYPVCDGDLDKALGIVYIKNLFLASDEEKYG
                     KLRDYIKPVLFLPENNTSYTVLEKFKQSKVHYALVVDEYGSVQGLVTINDILEAIVGE
                     FDELNLPDYSIIRRDDGSFLIDAGLPFYDFLVYFDIEEFDNLEELEYNTVAGLIIQEL
                     DRIPSEGEKLTWKDNEFEVIDMDGSRIDKVLFRPAEPNENES"
     gene            4376..5215
                     /locus_tag="PK742_05060"
     CDS             4376..5215
                     /locus_tag="PK742_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75992.1"
                     /translation="MILIADSGSTKTNWALLKEATQAHTFSTAGFNPNYSSPEAFEGA
                     VREVFSAAQAALVSAVYFYGSGAGNLRNDAILQQLLGSCFVHAEIRVFNDLMAACRGL
                     AGNQPGVVCILGTGSNACLYDGVNMSHDQYSLGYLLGDEGSGFAFGKRMLAAYLYEQM
                     PADLMAAFSASYPVTRDAVLHMAYQEQSPNKHIAGYSRFCSAHADHPWMAQQISDELE
                     RFYQAFLRRSETSGLPVHATGSVAAVFEDQLRKVCSSHGRLTGQIHADPLPGLIRFHQ
                     QKH"
     gene            5218..6870
                     /gene="pgi"
                     /locus_tag="PK742_05065"
     CDS             5218..6870
                     /gene="pgi"
                     /locus_tag="PK742_05065"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQU75993.1"
                     /translation="MFPDINGIETPAFASLQQHAETWKTQHMRALFDQDPTRFDRFSF
                     HQEGMLVDFSKHMITDETLTLLTDLAASCQLKEAMDALFSGEPVNRTENRAVLHTALR
                     DQSDRMVVVDGEDVKPAIRQVLEKMRLFCNEIHAGIRTGADGRSYTDVVNIGIGGSDL
                     GPAMVCEALKPFHKKGIRVHFVSNVDATHLVECVQDLDPATTLFIIASKTFTTQETMT
                     NARSAKQWFLESGMQEEDIARHFVAVSTALQEAQAFGIDPDNIFGFWDWVGGRFSLWS
                     AIGLSIMLAIGADGFDSLLNGAYAVDEHVRTAPLNKNIPVIMGLLGWWYNQFFEARTH
                     AVLPYDQYLEKFPAYLQQADMESNGKRIDRRGKEVSYPTGPVVWGAPGTNGQHAFYQL
                     IHQGTQLIPCDFIVPARSQNPLGDHHAILLSHCLAQSEAMMKGKTAEEVREELEHSGM
                     TEEEIQFQLPYRVFPGNIPSTTIIVPECNAFQLGQLIALYEHKIFVQGVLWNVFSFDQ
                     WGVELGKKLAAKILPEISGRKEPSGHDGSTIGLIKEVLRLRS"
     gene            complement(6867..7835)
                     /locus_tag="PK742_05070"
     CDS             complement(6867..7835)
                     /locus_tag="PK742_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HQU75994.1"
                     /translation="MNLVRDILRNPAFDFSVTCLQTFQEQAAANPVYKAWLEGIQTDP
                     QQITHPYEIPFLPVELFKTHTIRTGHFAPELSFMSSGTTGQRSTHDIASASDYTAVLL
                     EGFRITFGPPEQYRILCLLPSYHETGHSSLVFMCRQLMQFSAHPDNGFYHGQTNRLLD
                     LLYAPCERPTILIGVSYALLDLAENVHAPLQDTIVVETGGMKGRRVEMIRDELHEALC
                     AGFGLDTIYSEYGMTELLSQAWSSGGGIFYPPPWMQIQIADAEDPFHMLPPGRTGLLQ
                     IIDLANQHSCSFIGTRDLGRLRDDGGFEVLGRADNSDIRGCNLLIT"
     gene            7882..8331
                     /locus_tag="PK742_05075"
     CDS             7882..8331
                     /locus_tag="PK742_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75995.1"
                     /translation="MSAKWYIAAGVGLLFLASCIKPPEYSEVPYLEFSEINKTSFVEY
                     DPDSLKLTIYFEDGDGDIGGAEIDSLNMFFEDSRVPGYFVPFKIPFVELQGNHKAISG
                     YIHVTRGISQCINPVPVDTFHYNIQIRDRAGNYSNIIRTPDLLLNCD"
     gene            complement(8339..8524)
                     /locus_tag="PK742_05080"
     CDS             complement(8339..8524)
                     /locus_tag="PK742_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU75996.1"
                     /translation="MTTPSPPLMIFLTDRVDEQKEISGTPHTGNLYLPAVPYGNFCRF
                     EDWLSGSRKWRIDLGTD"
     gene            complement(8625..9260)
                     /locus_tag="PK742_05085"
     CDS             complement(8625..9260)
                     /locus_tag="PK742_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQU75997.1"
                     /translation="MIRSALLFLVFCTGASQLLAQQQQVHGWIQAGAEFDLTKDLELQ
                     VSLMARPEIYPDYTLTQYNTEIGLAYAWNKHWKAGLEGRFGLDEGFPESRLAASVRYR
                     DGMGPWELQWRMKAQIQWTQFELPEKELRNRFLLGYEINKHLMPYLAAEYFLTADHAE
                     TSFREYRYMAGAEYELNKKQDLELTYIYSNAWNTGNQNIRHIASLSWKYNW"
     gene            complement(9257..9874)
                     /locus_tag="PK742_05090"
     CDS             complement(9257..9874)
                     /locus_tag="PK742_05090"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HQU75998.1"
                     /translation="MNEEKDFRIMEIKFFDPDFYDFILRYGFNLIIAFLLLKFIYRAD
                     KKNKNYAFTFYVFNTLIFFLCYLMNNIKLDLGFAFGLFAVFSILRYRTISIPLKEMTY
                     LFLVIGIAVINALTTKKVSFVELMFTNVTILAITYLLERLWYKEGLSERVLEYEKIEN
                     IKPQNRELLVQDLRDRTGLDIREVEIVQTDFVRDLARVRIFFKDK"
     gene            complement(9867..10610)
                     /locus_tag="PK742_05095"
     CDS             complement(9867..10610)
                     /locus_tag="PK742_05095"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HQU75999.1"
                     /translation="MQSLAPIRLEEMEAVELLNRKDTKFIFPASRINSVLNRLKDSYR
                     ILEVNGDRTTGYHNIYFDTADHFCYLQHHNGRLNRYKIRIRSYVNSGLTFLEIKFKSN
                     RFRTIKNRIQVPTGTTELRDEMKQFIRDHCPIDPDLLQPVVEISFDRVTLVSKDMTER
                     ATIDLNLKASSMGKELNLQHLAIAELKQDGIISGSLFKWVLRRNHGTEKRISKYCICL
                     SATHPELRQNTFRIKHHYIRKLEKSDTYE"
     gene            complement(10628..11029)
                     /locus_tag="PK742_05100"
     CDS             complement(10628..11029)
                     /locus_tag="PK742_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76000.1"
                     /translation="MRGRFLYLPAIILAACLLNSCALGPGEGGKSTITGKVFIQEYNS
                     GGILVNEYYVADERVYLIYGDDTTYDDEFRTSYDGSYTFPYLRKGSYRLFSYSECLGC
                     DGSNEPVFVDVTINKNGEEISAPDIVIINYI"
     gene            complement(11086..>11371)
                     /locus_tag="PK742_05105"
     CDS             complement(11086..>11371)
                     /locus_tag="PK742_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76001.1"
                     /translation="LQPTYCGQLPVHLSRSSSELLLVAYITLAGTRKKEQIYAHIQHT
                     GQGNYRISGSHRLYFSHYGLKPPSAMFGLIQVNDAIDLYFDFLLQVTETP"
BASE COUNT         2941 a         2635 c         2741 g         3054 t
ORIGIN      
        1 atgacaaaat aggtcttctc aacttcacgc tctgcgatga tcctgctcat tcgttcggct
       61 gccttgccgg tttttgcaaa cagcagtaca ccacttgctg gcctgtccag acgatgcagc
      121 acacccagat acacattccc gggcttttga tactttacct ttaaatattc actcaccagc
      181 tcaccaatgg ggatatcgcc ggtcttgtct ccctgagaga tctcgccggg caatttgttg
      241 acggcgatca ggtggttgtc ttcatataag atcctggcag gatccatcat ggcagaatgg
      301 tttttctaac cttcgcaggt aagcttgcca ggatcagatc ataagaatgg tcgatccatt
      361 ccaatagaag tttgtcagga atactgccat ccacaattac cgtgttccag ttcattttat
      421 tcatgtggta acccggcaac acgtgcatgg ggtacttgtc tcgcagcaac agagcatctt
      481 ccggatcgca tttcaggttc atgcgcattt cgggtgtgtt cagtggggcc aacgcaaaca
      541 ttttgccacc tactttcatg accagtacat caggcccgaa tggtaagcct tctgttacac
      601 cgggtttggc aagacaatat tctctgaatg attccaggtg gagcataggt tgtaaaaata
      661 aaaaagcgca gacagtgctg cgcttttaat tatttgcttt tcagatcagt tcttcagcat
      721 acttctttgt gcggtatgtg catcatcgtt gattcgaatg gtgtacatgc cagagggtaa
      781 tcccatcaca ggcagagagg tctggctgat ggtacccgga gtattgcgca gatcatcaga
      841 gaatacgatg cggccagaca tgtcaattat atcaacatgc aacaactggc ctgaagcaaa
      901 gttgtaagag atattgaccc agttgtctgc gggattcggg tacacttcaa accaatagtt
      961 gtctgaaaga tcatttaccg ctacggtacc actggcaata ttgatattat ccagatacag
     1021 attgttttcc cagctgctca ctgtgcggaa cttcacaacc agcttatcgc caatgtacgg
     1081 tgtcaggctc aggctttcca gtcgccactg gtcattggca ggaataaatg cagcacctgt
     1141 tgcaggggcc gtattcaggt ctggccaggc tttgtaataa atacgggtcc agttgactcc
     1201 gcaatcagta ctgaccagta cttccagcga atcagagtat tctccacccg gcgtatagtt
     1261 ggcgtaggcc acatcaaaat ccaggtgggc agcaggaatg ccggtcatat tataggcagg
     1321 cagttcgagt tcatcaacag caccgatagc agtattctca aagtgattca tgaacacgga
     1381 ggcagaacca gttttggcag ctgctgttgt tctttcccag gtaaagctgc catcagggtt
     1441 atggacggta tagccggcat aggggaatgg atctacttca aatccctgaa taagcgggag
     1501 ggtgagcccg aaggtattga taaagaaatc actgctggcc tcgtcattgc tgctgttttc
     1561 atcaggttgt ccgtttggat cgaccacggt aactacgatg ctgtgtgcac catctccaag
     1621 agaaatggaa ggcagtgtca catcttccgt ttcataggtt tccagtgaac cagtccagaa
     1681 ataggttaat ggagaggctc cgtcaatgct gtattgaata tctacactgg tcatggtttc
     1741 cagaccggta ttgcggatgg taacaatagg agagaaggtg gagaaacaat agttgccaac
     1801 aggcgatacg atctccagtg cctctgcatc aaaattataa ctcacacagg caagagaatt
     1861 acccagggca aacctggctc cgcctgcttc aaatatggca cgggctctgt ctttctgtcc
     1921 ttcggtaaac atattgtagc aggcatcatc tgtatagtcc atataattct gaaactgtat
     1981 ggactcactg caggcatctg ccagcggata ggtaggacaa ccaaaggtct gtacatcctg
     2041 tacgggggtg tcatctactg aatcatctgt tccgcaaccg gctgcatcac cccagatatg
     2101 gtagaggttc agccagtgac ctacctcatg cgttcctgtt cgaccgaggt cataaggtgc
     2161 aattacatat ccttcacggc caaagctgga ataagtgatg accactccat cagtttcatc
     2221 gggccctccg ggaaactgcg cataacccag taaacctgaa cccagatcgc agctccacat
     2281 attcatatat tcatcggcag gccaggcgtc atcgccacct gaagcagtga attttacatc
     2341 atcactgatg ccgaatgaac tggacgtagt tggtattctt acaataccgg tggtcgggtt
     2401 tccgtccgga tcgaccgtag ccatgcaaaa ctcaatttca aaatcagccg caactgcatc
     2461 aaactctgct ggtgtatctg cagcatctgc attgagtctg cgcagatctt cattaagtac
     2521 atcaatctgt gagagcacct gggcatcgga gatgttttcc aggtcatttt catataccac
     2581 atgcactaca accggaatgg tgatgacatc agctctggta ccactggcca ggtgcttatt
     2641 gatatatttc tgggtgaaat cttcaacata tgcacggcgt gctgtgtaac cggcattttc
     2701 ttcctgcagg cgatggtcca catcaggagt tccgcactgt tctgccgact gtgcatacaa
     2761 accggaagta ataatcacgg acaggaggag taatacagtt cttttcataa gaatggtatg
     2821 taggatgtat ttacagtcaa aattactgaa tatcagtgac caaatagagt aaaaacaagg
     2881 tgcaaaacct aattatgacc ttttgacatt atcatgaaat agaccgattc tgttcaaata
     2941 gctaattttg cgcaaagacc tgttctgatg tctgctgctg acgaccccgg cccgcccccg
     3001 aaataccctt tcatttcata tctacagacc tcctttggca taaggcggaa ttacctattt
     3061 ttaagcgatc acgcatgata accgatttcc tgatcatttt tctgttgata tgcctgaacg
     3121 ggcttttcgc catgagcgag atcgctatga tctcactgcg taaaagcagg ctggaaacag
     3181 ccgcaagaaa gggcgataag agagctgctg cagcactgga actgcacaac caacctggca
     3241 gattcctctc tactgtccag ataggaataa ccctgatcag catcctgacg ggtatatatt
     3301 ccggtgatac cctgaaagag cccattattg gctggctttc tcagtgggat gcagttgcgg
     3361 tttatgcaga tagtctggcg gtcaccattg tggtcatctt cataaccggc ctgtctctcc
     3421 ttgtgggtga attgctgcct aaaagaattg gcctcagcta ttcagaatcc atagccaaga
     3481 tcatggcagc acctatgacc atgctgtcaa aagttactgc ccctttcagc tgggtgctga
     3541 catctgccac caatggcctg atacgtctca tgaggctgaa gcactcgaat gatgaccggg
     3601 taacagaaga agagatcaag gccatgatac aggagggaac tgactccggc gaaattgaag
     3661 agatcgaaca ggacattgtg gagcgggtgt ttcatctggg cgacagaagg atcgcatcac
     3721 ttatgaccca ccgcagtgat attgtatggc tggatatcaa ttcgggtcct gaagaactga
     3781 agcataccat ctccacagag gtgcactctg tctatcctgt ttgtgacgga gacctggaca
     3841 aggcacttgg tattgtttat atcaagaacc tgttccttgc aagcgacgaa gagaaatacg
     3901 gaaaactgcg tgattatatc aagccggtgc tgtttctgcc ggaaaacaat acttcttata
     3961 ccgtactgga aaagttcaag caatcaaaag tgcattatgc cctggtagta gatgaatacg
     4021 gttcggttca gggcctggtt accatcaatg atattctgga agctattgta ggcgagtttg
     4081 atgaactgaa cctgcctgac tacagcatca ttcgccgcga cgatggcagc tttctgatag
     4141 atgctggcct tccattctat gacttcctgg tgtactttga tattgaagaa tttgacaacc
     4201 tggaagaact ggaatacaac acggttgccg gtttgatcat acaggagctg gacaggatac
     4261 cctctgaagg ggaaaagtta acctggaaag acaatgagtt tgaagtgatc gatatggacg
     4321 gttccaggat cgataaggta ttgttccgtc cggctgaacc caacgaaaac gagtcgtgat
     4381 ccttattgct gacagtggtt ccaccaaaac caactgggct ttgctgaaag aagcaactca
     4441 ggcgcatact ttttcaactg caggcttcaa tcccaattat tcttcacctg aagcgtttga
     4501 aggtgccgtc agagaagtgt tcagcgctgc gcaggcagct ttagtttctg ccgtatattt
     4561 ttatggttca ggagccggaa accttcgcaa tgatgccatc cttcaacaat tactgggcag
     4621 ctgttttgtt catgcagaga tcagggtgtt caatgacctg atggctgcct gtcgcgggtt
     4681 ggcaggaaac cagcctggtg tagtctgcat tcttggcaca ggttccaacg catgtttgta
     4741 tgacggtgtg aacatgtcac atgatcagta cagcctgggc tatctgctgg gggatgaagg
     4801 cagtggtttt gcctttggca agcgcatgct cgcggcctat ctgtatgagc agatgcccgc
     4861 tgacctgatg gctgccttct ctgcctccta tcctgttacc agagatgcgg tgttgcacat
     4921 ggcttaccag gagcaatcgc ccaataagca cattgccgga tatagcagat tctgcagtgc
     4981 acatgctgat catccctgga tggcacagca gatttcagat gagctggaac gtttctacca
     5041 ggcattcctc cgtcgttctg aaacctccgg gcttcctgtt catgcaaccg gttctgtagc
     5101 agccgttttt gaagatcagt tgcggaaggt atgcagcagc catggccggt taaccggtca
     5161 gatccacgca gatccgcttc cgggtctgat acgttttcat caacaaaaac actgactatg
     5221 tttcctgata taaacggtat tgaaacacca gcttttgctt ctcttcagca gcatgcagag
     5281 acatggaaga cacagcacat gcgtgcactt tttgaccagg accctacacg ttttgatcga
     5341 ttttcttttc atcaggaggg catgttggtt gatttctcca aacacatgat cacagatgaa
     5401 acacttacac tgcttacaga tcttgccgct tcctgccagc tgaaagaggc catggatgcc
     5461 ttgttttcgg gggagcctgt caaccgtaca gaaaaccgtg cggtactgca tactgccttg
     5521 cgagatcaga gcgatcggat ggtggtggtg gatggtgaag atgtgaagcc tgcaatcaga
     5581 caggtgctgg aaaaaatgcg tctattctgc aatgagattc atgccggaat tcgcactggt
     5641 gccgatggca gatcctacac cgatgtggtc aatattggca taggaggatc agacctcggg
     5701 cctgcaatgg tgtgtgaagc actaaaacca tttcacaaga agggaatccg ggtgcatttt
     5761 gtgtctaatg tagatgcaac acatctggta gaatgtgtac aggatctcga tccggctacg
     5821 accttgttca tcattgccag taagaccttt accacccagg agaccatgac caatgcccgt
     5881 tctgcaaagc aatggttcct ggaaagcggc atgcaggaag aagacattgc caggcatttt
     5941 gtagccgttt ctacagcctt acaggaagct caggcattcg ggatagatcc ggacaatatt
     6001 tttggtttct gggactgggt tggcggacga ttttcacttt ggagtgctat tgggctgagc
     6061 atcatgctgg ctattggcgc cgatggtttt gattcattac tgaatggtgc atacgcggta
     6121 gatgaacatg tcagaacagc tcccttgaac aagaacattc ctgtcatcat gggtttgctg
     6181 ggctggtggt acaaccagtt ttttgaagcc cgcacacatg cagtcttacc ctatgatcag
     6241 tacctggaga aatttcctgc ctatctccag caggcagata tggaaagcaa tggaaagcga
     6301 atagacagaa gaggcaagga ggtcagttat ccaaccggtc cggtggtttg gggtgcaccc
     6361 ggtaccaacg gccagcatgc tttttatcag ctgatccatc agggaacaca gctcattcca
     6421 tgtgatttca ttgttccggc ccgatcgcag aatccgttgg gtgatcatca tgccattctt
     6481 ctgtctcatt gtctggcaca aagtgaagcc atgatgaaag gaaagacagc agaagaagtt
     6541 cgtgaagaac tggagcattc cggaatgacc gaagaagaaa tacagtttca gctgccctac
     6601 agggtgttcc cgggaaatat cccatctaca acgatcattg ttcctgaatg taatgccttt
     6661 caacttggcc agctcattgc cctttatgaa cacaagatct ttgttcaggg tgtactgtgg
     6721 aatgttttca gctttgacca atggggtgtg gaactcggca agaagcttgc agctaagatc
     6781 ctgcctgaaa tttcaggcag aaaggaaccg tccggacatg atggatctac catcggcctg
     6841 atcaaagagg ttcttagact aaggtcttaa gtgatcagga gattgcatcc tctgatatca
     6901 ctgttgtctg cacggccaag cacctcaaat ccgccatcat ctcttaacct tcccaggtct
     6961 cttgtgccaa tgaaagaaca ggagtgctga ttggccaggt caatgatctg caggaggccg
     7021 gttcttccag gtggtaacat atggaatggg tcttcagcat cggctatctg aatttgcatc
     7081 cagggtggtg gatagaatat acctccaccg gaagaccatg cctgcgacag taactctgtc
     7141 atgccatatt cactgtaaat agtatccagt ccaaaacctg cgcaaagagc ctcatgcagt
     7201 tcatctctga tcatttcaac acggcgccct ttcatccctc cggtttctac aacgattgta
     7261 tcctgaaggg gtgcatgcac attttcagca agatcaagca aggcatagct gacacctatc
     7321 agaatggtag ggcgttcgca aggtgcatac agcagatcca gcaaacggtt tgtttgcccg
     7381 tgatagaaac cattgtcagg atgcgcactg aattgcatca gctggcggca catgaaaacc
     7441 agactggaat gcccggtttc atgatagctg ggaagcaggc aaagtatccg gtattgttcg
     7501 ggtggcccaa aagtgatccg gaatccttcc agcagaacag cagtataatc gctggcagat
     7561 gcaatgtcat gtgtggaacg ctgccctgtt gtaccactgc tcatgaagct cagttccggt
     7621 gcaaaatgcc cggttctgat cgtgtgggtc ttgaacaact ctacaggcag gaatgggatt
     7681 tcatacgggt gggtaatttg ctgcggatct gtttgaatgc cttccagcca ggctttgtac
     7741 acagggttgg cggcagcctg ttcctgaaag gtttgcaggc atgtcacgga aaagtcgaaa
     7801 gcaggattcc gcaggatatc gcgaaccaaa ttcaccgcat aaagttatac taatgtggta
     7861 tttttagagt tgataatgcg tatgtctgcc aaatggtata tcgctgcagg agtaggcttg
     7921 ttgtttctgg catcctgcat caaaccaccg gaatattctg aggtgcctta cctggaattt
     7981 tcagagatca acaagacctc ctttgtagaa tatgatcctg acagcctgaa gctgaccatc
     8041 tactttgaag acggtgacgg agacattggt ggggcagaga ttgacagcct gaacatgttt
     8101 ttcgaagaca gcagggttcc gggttatttc gtaccgttta agattccgtt tgttgaactg
     8161 cagggaaacc ataaggcgat atcaggttat attcatgtga ccagaggtat ctctcaatgt
     8221 atcaatccgg taccggttga tacctttcac tacaatattc agatccgtga cagggcagga
     8281 aactacagta atatcattcg cacccctgac ttgctgctta actgtgatta agggctgatc
     8341 agtctgtacc caggtctatc ctccatttcc tgctgccact cagccagtct tcaaaacggc
     8401 aaaaattgcc gtaaggaact gccggcagat agaggttgcc tgtgtgaggg gttccggata
     8461 tttctttttg ttcatccacc ctgtctgtca gaaagatcat caggggtggg gaaggtgtag
     8521 tcatgtctgt taaaaagaaa gtgtcagcca acacgatacc gggagtgctc tgctgggaat
     8581 atccgggaat tgtcagaaaa aaaagcagac aacaggccag tatattacca gttgtacttc
     8641 cagctgagtg aagcaatatg cctgatgttt tggtttcctg tattccaggc attgctgtaa
     8701 atatacgtga gttccagatc ctgcttttta ttgagttcat attcagcacc tgccatataa
     8761 cggtattctc tgaaagatgt ttctgcatgg tctgctgtca gaaaatattc agctgcaagg
     8821 tagggcatca ggtgtttgtt gatctcatac cctagcaaga agcggttgcg cagttctttt
     8881 tccggcagtt caaactgggt ccattgaatc tgagccttca tgcgccattg cagctcccag
     8941 gggcccatgc catcccggta tcttaccgaa gccgccagtc tgctttccgg aaaaccctca
     9001 tcaagcccga agcggccttc cagtcctgct ttccagtgtt tgttccaggc atatgcaagc
     9061 ccgatctctg tattgtattg tgtcagggtg tagtcagggt atatctcagg tctggccatc
     9121 aggctgacct gcagttccag gtctttggtc agatcgaatt cagcacctgc ctggatccag
     9181 ccatgcactt gctgctgctg tgccagtagc tgagaagcac cggtacagaa aaccagaaac
     9241 aacagggcgg atcgtatcat ttatccttaa aaaagatcct gacacgagcc agatcacgta
     9301 caaaatctgt ctggacgatc tccacttctc tgatatcaag gcctgtccgg tctctcagat
     9361 cctgtaccag gagttcacgg ttctgcggct ttatgttttc tatcttttca tattccagca
     9421 cacgttctga cagcccctct ttataccaca gtctttccag aagataggtg atggccagaa
     9481 tggtgacatt ggtgaacatc agttctacaa agcttacttt tttggtggtc agtgcattaa
     9541 tgaccgcaat accgatcacc agaaacagat aggtcatttc ctttagcgga atactaatgg
     9601 ttcggtatcg aaggatggag aatacggcaa acaaaccaaa tgcgaaacca aggtccagtt
     9661 tgatattatt catcagatag caaaggaaaa agatcagtgt attgaagaca taaaaagtga
     9721 atgcataatt cttgttcttc ttatcagcac ggtatatgaa tttgagtaac aggaatgcta
     9781 tgatcagatt gaatccgtaa cggagaataa aatcatagaa atctggatca aagaacttga
     9841 tctccatgat ccggaagtct ttttcttcat tcataggtgt ctgatttttc taattttctg
     9901 atataatgat gtttgatcct gaaggtgttc tgtcttaatt caggatgtgt ggctgataga
     9961 caaatgcagt acttgctgat ccgtttttca gtgccgtgat ttctgcgtaa aacccacttg
    10021 aacagcgaac cggaaataat gccatcctgc tttagttctg ctatggccag atgctgcagg
    10081 ttcagttctt tgcccatact ggatgctttc aggttcaggt caatggtagc tctttcggtc
    10141 atgtctttac ttaccagagt aacccggtca aaactgatct ccacaaccgg ttgaagaaga
    10201 tcggggtcga tcgggcaatg atcccggatg aactgtttca tttcatcacg aagttcggtg
    10261 gttccggttg gcacctgaat cctgttctta atggtacgga aacggttgct tttaaatttg
    10321 atctccagaa aagttagtcc ggaattgaca taggagcgta tccggatctt gtatctgttc
    10381 aaccttccgt tatggtgctg gaggtaacag aaatgatctg ctgtatcaaa atagatattg
    10441 tgatatccgg tggtccggtc tccgttcact tccagtatcc tgtaggagtc tttcaacctg
    10501 ttcagaacgc tgttgatacg cgaagccgga aagatgaatt tggtatcctt tctgttgaga
    10561 agctccaccg cctccatctc ttccagacgg atgggtgcaa ggctttgcaa tatgtctgat
    10621 gccggattca gatatagttg attatcacaa tatcaggagc tgaaatttct tctccgtttt
    10681 tattgatggt aacatctaca aaaacaggtt cattggaacc atcgcaaccg agacattctg
    10741 aataggagaa caaacgatag ctgccctttc tcagatacgg gaaagtatag cttccgtcat
    10801 aacttgttct gaactcatca tcgtaggtgg tatcatcgcc ataaatgaga taaacccttt
    10861 cgtctgccac atagtactcg ttcaccagaa tgccaccgct gttgtattcc tgtatgaaca
    10921 cttttccggt gatggtgctt ttccctcctt caccgggccc cagagcacaa ctgttcaaca
    10981 ggcaggcggc cagtatgatg gcaggtaaat acagaaatct ccccctcatt aagattaatt
    11041 taacatacaa atgtagcgat tgccgcaata attcctgatg gcagatcagg gtgtttctgt
    11101 aacctggagc aggaagtcaa agtacaggtc tatggcatca ttcacctgga tcagtccgaa
    11161 catggctgat ggtggtttca ggccatagtg actgaagtac agacgatgac tgccactgat
    11221 cctataattt ccctgacctg tgtgctggat atgggcatag atctgctctt tttttctggt
    11281 gccggccagt gtgatatagg ctaccaggag cagttcactg gaagatctgg acaagtgcac
    11341 cggaagttga ccgcaatagg taggctggag c
//
LOCUS       DAOMAX010000063        11232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_773_length_11232_cov_4.660821, whole genome shotgun sequence.
ACCESSION   DAOMAX010000063 DAOMAX010000000
VERSION     DAOMAX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_11232_cov_4.660821"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PK742_05110"
     CDS             <1..1228
                     /locus_tag="PK742_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQU76002.1"
                     /translation="INNVVNRGMQLMVQVIKEPIQTKGPRLSCELSLPGRFLVLTPFN
                     DVVGVSRKIESADERKRLQKIIESIRPKNYGVIVRTVAEGQSVQTLHNDLMDQVKKWE
                     EITKKAEGAVPPQVLFSEATKTNTLLRDILNDSFNSIVVNDQSMANDVKNYIKRIAPG
                     AEKMVAVHSGNRSIFDANGVTRQIKSLFGRTVNMSSGAYLIIEHTEALHVIDVNSGNK
                     SAVRGDQESNAVAVNLEAAREIARQLRLRDIGGIIIIDFIDMKKPDNKKLIYTELRKA
                     MESDRAKHSILPISKFGVAQITRQRVRPEVNIATAEVCPTCNGSGKIEASILILTELE
                     RKLGYLYQQQNQKKLKLVVHPFVEAYIKKGRIFRTLQWKWYRQFGQMLKVVGNDDYQY
                     MEYHFFDVNDEEIQVE"
     gene            1251..1871
                     /locus_tag="PK742_05115"
     CDS             1251..1871
                     /locus_tag="PK742_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQU76003.1"
                     /translation="MQNEALQVLQDSKLIEELRSFSETTTFPPDAVILQQMDYIRYLP
                     FLLSGSIKVMGEDEEGHEIMLYYLHPGETCIMSVLGALNNEKSKIRAVIDEEAEVMMI
                     PVEKTQYLVSQFPSWSAFIFRLYHKRFEELLRIVNSIAFQKVDKRLEDLLESRSVASK
                     TTELHVTHKQLADELGTAREVVSRLLKQMEREGKLELGRNRITLLQ"
     gene            1930..2220
                     /locus_tag="PK742_05120"
     CDS             1930..2220
                     /locus_tag="PK742_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQU76004.1"
                     /translation="MYLEDLLKEDVAIIDVRTPMEFMGGHVAGSINIPLNEIMSRVDE
                     LKEMAKPLVLCCASGNRSGQAAMALSQLGMKEVYNAGSWLTVNAVQSQVQQS"
     gene            2217..2546
                     /locus_tag="PK742_05125"
     CDS             2217..2546
                     /locus_tag="PK742_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQU76005.1"
                     /translation="MREKLMKWLGLTPVNYSDLLDRGAVIVDVRTKGEYSSGHIRGSV
                     NIPLDRLSDQMKKLQKNKPVITCCASGMRSASAKGMLQSKGFEVVNGGAWHSLQRKLI
                     NLTEKSA"
     gene            2543..2995
                     /locus_tag="PK742_05130"
     CDS             2543..2995
                     /locus_tag="PK742_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQU76006.1"
                     /translation="MKKTAALRLSYLLVGLSTFGFSACAQTGGEEQRDTTMTIEAAGE
                     VVDLPLARFHDRMMEQPGQLVDVRTPEEYAGGHAQGASNINFLDKKFLEEFEAAIDKD
                     KPVYIYCASGNRSGKAKSALEKAGYKYIYNLTGAGYAQWAAAGYPTEQ"
     gene            2998..3873
                     /locus_tag="PK742_05135"
     CDS             2998..3873
                     /locus_tag="PK742_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQU76007.1"
                     /translation="MNYLQALENGISRTFHWTWQLVSMQVPWYLNFFYGLIIISLVVW
                     MLEIAFPWRKQQGTFRKDFWLDGFYMFFNFFLFAIGLNGIWAMFGQLGRDLHFTAGSL
                     TLVDMSAWPSWAQLLVFFLILDFVQWFTHILLHRIPAFWQFHKVHHSVQEMGFAAHLR
                     YHWMENVLYKPLKTLGVMVIGGFEPEQAYLVHFIALTIGHFNHANIHITWGPLRYILN
                     NPVMHLWHHAKELPSNHPYGVNFGISLSVWDYIFRTNYIPKDDPDIPLGFPGVEEFPD
                     TFGKQFISGFGKHRT"
     gene            3870..4097
                     /locus_tag="PK742_05140"
     CDS             3870..4097
                     /locus_tag="PK742_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76008.1"
                     /translation="MTFFSKHISTFLLLSLTLGLAPFSPEPHIWGKIRWIRGGAIGMT
                     GMDWFDVLLHGTPWVLLVLALIAKATEKVRG"
     gene            complement(4087..4821)
                     /locus_tag="PK742_05145"
     CDS             complement(4087..4821)
                     /locus_tag="PK742_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQU76009.1"
                     /translation="MEESNSLHHDQEQNVSAGGLSRYLPEFVYGGIDGSVTTFAVVAG
                     AAGAGLEPRIVLILGFANLFADGFSMSVGAFLSAKSSLAMYHKHRRREYWEIDHLRDA
                     EVEEIRQIYRNKGFEGELLEQVVARITEDKDRWVDVMMKEELEMIPESKSPGMIGLAT
                     FISFFLVGLLPLIIFILSYFMPVPQQKYLISAGITLLGFIGIGYMKSHVTETSVSRAI
                     GETVLLGAIAAALAYLVGDLLEKIIL"
     gene            complement(4867..6507)
                     /locus_tag="PK742_05150"
     CDS             complement(4867..6507)
                     /locus_tag="PK742_05150"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQU76010.1"
                     /translation="MRIALAQLNFHIGNFEHNFSKMEARVQEAKEAGADLIVFPELAV
                     CGYPPRDFLEFEDFIDKCEEVVNRLAAFSKDIGIIVGSPARNPVVEGKDLFNAAFLLA
                     DGKIAGQANKALLPTYDIFDEYRYFEPNDQFSVVEFRGKRIALTVCEDIWNVGNENPL
                     YKSCPMDTLRTQSPDLMINISASPFDYGQAHDRIEVVRANTLAYALPMIYVNHVGAQT
                     DLIFDGGSLAVSAGGHVFLEMPYFEEALHIIELEKAESYTGAPNVNGKDKIGLIHDAL
                     LSGIRDYFGKLQFNKAILGLSGGIDSAVTALLAARALGPEHVHCVLMPSPFSSGHSVS
                     DSEALCRRNGISYTIIPIDNMYEQMVAQMHPVFGDLSFDVTEENMQARIRGMTLMAMC
                     NKLGYILLNTTNKSEAAVGYGTLYGDLCGGLAVLADVYKTEVYQLASYINKDSELIPE
                     NIIHKAPSAELRPNQTDQDSLPPYDLLDQILFQYIEKHQSPEDIIDKGFDQEIVRKTL
                     RMVNRNEWKRFQMAPVLRVSPKAFGMGRRMPIEGQYLS"
     gene            complement(6558..7241)
                     /locus_tag="PK742_05155"
     CDS             complement(6558..7241)
                     /locus_tag="PK742_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQU76011.1"
                     /translation="MKKIALLSCLFIFLAGSVKAQDFRFGLTAAPLISWFNVDGTDVE
                     NDGTRLGFQYGLLFEPTIGSVERYAISTGVIVNMLGGFLTAEDTTLGNINTTLRVQYI
                     EIPATIKLRTNEINYFTYYGMFGLNLGMNIKARYDVTDGSGNYLPDGNGGELQDVNLN
                     DKNSIDQYKFFNLALSMGIGTEYSITETTTLTAGLVFQNGFVNVFETAATDEMIQMKQ
                     MVLRMGVLF"
     gene            7263..8327
                     /locus_tag="PK742_05160"
     CDS             7263..8327
                     /locus_tag="PK742_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76012.1"
                     /translation="MNTDRFYRHKHKAFSIIPIVLAVSILLVSGCRKGPQLPHPDVSG
                     IEVDASIHRFDEALMAADGSNYREVMLAMELQDSAFFHLYTGQILNMRPYGDSTFSLY
                     DTLYKYMVADPYMIRLYDSTKALFPDMRSQEKALQKALKYYSYYFPEMQIPSFYAYIA
                     PFVYQVVVADEVIGIELNMFMGKDFSYYSELSANLPQYLVYRFDPDYMVVSVMRAMMD
                     GSIPNKGADGTLLDDMLAEGKMMYYLDLMLPDLPDSIKIGFTEEQLNFCYDNESDIWK
                     FFAGEELLFSTRNQDRQRYLGESPGAYGMPEGAPGRIGIWVGWQMVRAYMDAHPETNI
                     HQLLLMTEGLDFLQESGYKP"
     gene            complement(8324..8716)
                     /gene="apaG"
                     /locus_tag="PK742_05165"
     CDS             complement(8324..8716)
                     /gene="apaG"
                     /locus_tag="PK742_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQU76013.1"
                     /translation="MDMVTATTDGVKVSVETFYQKDYAGMATAEHVFAYRITIENKGP
                     VPVRLLRRHWHIFDSSNDWREVEGEGVVGEQPLIEPGGLHQYVSGCSIRGMAGKMFGT
                     YTMERAHDNETFQVLIPEFRMIVPAVLN"
     gene            complement(8756..9319)
                     /gene="gmk"
                     /locus_tag="PK742_05170"
     CDS             complement(8756..9319)
                     /gene="gmk"
                     /locus_tag="PK742_05170"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQU76014.1"
                     /translation="MSRQLLIITAPSGAGKTTIVRYLLGHFRQLAFSISATTRPKRDH
                     ETDGVDYYFLDHDTFQEKIRQHAFAEYEEVYPGRWYGTLRTEMDRLWNDDKCIVFDID
                     VLGALNLQSQYPEESLSMFIAPPSLEVLENRLRSRGTETPESLTARLQRAAMELERCN
                     SFDQVIINDDLETACQKAARIVTAFLS"
     gene            complement(9316..10179)
                     /locus_tag="PK742_05175"
     CDS             complement(9316..10179)
                     /locus_tag="PK742_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HQU76015.1"
                     /translation="MTGYGRSRLPINGKTYTVEVRALNSKGLDINVRLSNMFREREMD
                     IRNLVSGLVIRGKVDILINNDPGEEQRSADLDPALISAYVQRLREAAGTILVSDEKLF
                     ELAAQMPNVAGQTQETLLPEQWSTVAKALQDACAELTVFRKQEGSNLQKDLELRTRNI
                     EDTLLAVEQLDPERRDRSKDKLMQQLNSQFSDEQIDKNRFEQELIYYLEKMDITEEII
                     RLRSHCTYFLENITQEAIEKGKKLAFISQEMGREINTIGAKSYHAGMQKLVVDMKDEL
                     EKIKEQLNNVL"
     gene            complement(10211..11107)
                     /locus_tag="PK742_05180"
     CDS             complement(10211..11107)
                     /locus_tag="PK742_05180"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQU76016.1"
                     /translation="MQTETRAHLYIFGANVIYGLNYVIAKVALSAIPPFALVLTRVSI
                     PLFIFGLLAVTLEKQSVKRKDHIKLFYCGLLGVAANQLMFIKGLDLTSEIHASLIMIT
                     TPILVLIMSWFFLRDPLTWKKAIGVLTGAAGVWMLIRSGARDAGSEATLGGDLLIMGN
                     AASYALFLVLAKPLMHNYKPFTVTFWLFLYGLIWVLPFGVPEALHMPWQGYAPGVMWS
                     WAYVVFISTLVVYIMNIIGLKLGNPALVSIYIYTQPLLATLVAVLLGTDQLTVTMVLA
                     GLLIFTGVALVSRKPVQKESIQ"
BASE COUNT         2887 a         2782 c         2763 g         2800 t
ORIGIN      
        1 gatcaacaat gtggtcaacc gcggcatgca actcatggtg caggtcatca aagaacccat
       61 ccagaccaag gggcccaggt taagttgcga actttccctg cccggcaggt ttctggtgct
      121 taccccgttc aatgacgtgg tgggtgtttc caggaagatc gaatctgcag atgagcgcaa
      181 aagactgcag aagatcatag aaagcatccg gccgaaaaac tacggtgtga tcgttcgcac
      241 agtagccgaa ggacaaagtg ttcagaccct gcacaacgac ctgatggatc aggtaaagaa
      301 atgggaagag atcaccaaaa aggcagaagg cgctgtacct cctcaggtgc tgttctctga
      361 agccaccaag acgaacaccc tgcttcgtga cattctcaat gattctttca acagcatcgt
      421 ggtcaatgac cagagcatgg ccaacgatgt aaagaattat atcaaaagaa tagcccccgg
      481 tgcagaaaag atggtagctg tgcacagcgg caaccgttcc atctttgatg ccaacggcgt
      541 aaccaggcag atcaagtcgc tgtttggccg cacggtcaat atgagcagtg gtgcctatct
      601 catcattgaa catacagaag cgctgcacgt cattgatgtg aacagtggca acaaatcagc
      661 tgttcgcggc gaccaggagt ctaacgccgt ggcagtgaat ctggaggcag ccagagagat
      721 cgccagacaa ctcaggttgc gtgatattgg tggcatcatc atcatcgatt tcatcgatat
      781 gaagaagccc gacaacaaaa agctgatcta cacagaattg cgcaaggcga tggaatctga
      841 ccgggccaag cactccattc tgcccatcag caaattcggg gtggcgcaga tcacccgtca
      901 acgggtgcgg ccggaggtga atattgccac ggcagaggta tgccctacct gcaatggttc
      961 gggtaaaata gaggccagta tcctgatcct gacggaactg gaaaggaaat taggatattt
     1021 atatcagcag cagaatcaga agaaactgaa actggtggtg catccctttg tggaagctta
     1081 tatcaaaaaa gggcgtattt tccgtaccct tcaatggaaa tggtacagac agttcgggca
     1141 gatgctgaaa gtagtgggca atgatgacta ccagtacatg gaataccatt tcttcgatgt
     1201 caatgatgaa gagatacagg tagaataaaa tcttttaagg tacccgaccc atgcagaacg
     1261 aagctttaca ggtattgcag gacagcaaac tcattgagga actgcgcagt ttctctgaga
     1321 cgactacttt tccacccgat gctgtcatcc ttcaacagat ggattacatc cgttatctcc
     1381 ctttcctgct gagcggaagc atcaaggtga tgggtgaaga tgaagaaggt catgagatca
     1441 tgctctacta tctgcatccg ggagagacct gtatcatgtc tgtgctgggt gctctgaaca
     1501 atgagaagag caagatccgt gcggtgatcg atgaagaagc cgaggtcatg atgatcccgg
     1561 tagagaagac ccaatacctg gtcagtcagt ttcccagctg gtctgccttc atctttcgct
     1621 tgtaccacaa acgctttgaa gagctgttgc gcatagtgaa cagcattgca tttcagaagg
     1681 ttgacaagcg tctggaagat ctgctggaaa gcagaagcgt cgcttccaaa acaacagaac
     1741 tgcacgttac ccataagcaa ctggccgatg agctgggtac tgcccgcgag gtcgtatcga
     1801 ggttgctcaa gcaaatggaa cgagaaggca aactggagct gggcagaaac cgcatcaccc
     1861 tgcttcagta acccgggtca ccaactgacc tgtccggccc tgttactttt gtccctcaaa
     1921 caaagaaaga tgtatctgga agatctgttg aaagaagatg tggccatcat agatgtgcgc
     1981 acaccgatgg aattcatggg gggccatgtg gccggttcca tcaacattcc cctgaatgag
     2041 atcatgagcc gcgtggatga gctcaaggaa atggccaagc ctctggtgtt gtgctgtgcc
     2101 tcaggaaacc ggagcgggca agccgcaatg gctttaagcc aacttggcat gaaagaggtc
     2161 tataacgccg gctcctggct tactgtcaac gccgtacaaa gtcaggttca gcaatcatga
     2221 gagaaaaact gatgaagtgg ctgggactta ccccggtcaa ctacagcgac ctgctggatc
     2281 gcggagctgt catagttgat gtgcgcacca aaggcgaata cagcagcggg catattcgcg
     2341 gatctgtgaa tattccgctg gatcgtttat ctgaccagat gaaaaaactg cagaagaaca
     2401 aacccgtcat tacctgctgc gccagtggta tgagaagtgc atcagccaaa ggcatgttgc
     2461 aatccaaagg ttttgaagta gtgaacggcg gagcatggca cagcctgcag cgcaaattga
     2521 ttaacttgac tgaaaaatca gcatgaaaaa aacagccgca ctcagactct cctacctgct
     2581 ggttggactt agcacctttg gcttttctgc ctgtgcacaa actggtgggg aagaacagcg
     2641 tgatacgacc atgacaatag aagcagccgg agaggtggtt gaccttccgc tggcccggtt
     2701 tcacgaccgc atgatggagc aacccggaca gctggttgac gttcggaccc cggaagaata
     2761 tgccggcggg catgcacaag gtgcttccaa catcaacttt ctggacaaga aattcctgga
     2821 agaatttgaa gccgctatag acaaagacaa gcccgtctat atctactgtg cttccggcaa
     2881 cagaagtggc aaagccaaat ctgctctgga aaaagcggga tataaataca tctataacct
     2941 gacaggcgca ggttatgccc aatgggcggc agccggctat ccaacagagc aataggtatg
     3001 aactatctgc aggcactgga aaatggtatc agcaggacct ttcactggac ctggcaactg
     3061 gtcagtatgc aggtgccctg gtatctgaat ttcttctatg gcctgatcat catttcactg
     3121 gttgtctgga tgctggagat cgcattcccc tggcggaaac aacagggaac tttccgcaaa
     3181 gatttctggc tggatggctt ttatatgttc ttcaactttt tcctgttcgc catcggactg
     3241 aacggcatct gggcaatgtt cgggcagttg ggcagagacc ttcattttac agccggcagc
     3301 ctgaccctgg tggacatgtc tgcctggcca tcgtgggcac aactgctggt gttcttcctg
     3361 atcctggatt tcgttcagtg gttcacgcac attctgttac acaggattcc ggccttctgg
     3421 cagttccaca aggtgcatca ctcggtgcag gaaatggggt ttgctgcaca tcttcgctat
     3481 cactggatgg agaatgtatt gtacaagcct ctgaagaccc tgggtgtcat ggtcatcgga
     3541 ggattcgagc cggaacaggc ctacctggta catttcatcg ccctgaccat cgggcatttc
     3601 aatcatgcca acatccacat cacctgggga ccgttgcgct atattctcaa taatccggtc
     3661 atgcatctct ggcatcacgc caaagagctg ccgtccaacc atccttacgg ggtcaacttc
     3721 ggcatcagtc tgagtgtgtg ggattatatc ttccgcacca actacatacc gaaagatgat
     3781 ccggatattc cacttggatt tccgggcgtg gaagaatttc cagacacctt tggcaagcag
     3841 ttcatctccg gattcggaaa gcacagaaca tgacattttt cagcaagcat atcagcactt
     3901 ttcttctgct gagtcttaca ttaggattgg cgccgttttc acctgaaccg cacatctggg
     3961 gaaagatccg ctggatacgg ggcggagcca tcggcatgac cggcatggac tggtttgatg
     4021 tactgcttca cggaacacca tgggtattat tggtgctggc attgatagcc aaagcaacag
     4081 agaaggtcag aggatgatct tttccagcag gtcgcccacc agataagcga gggctgcagc
     4141 tatggcaccg agcaatactg tttcacctat tgcccttgaa acacttgttt ctgttacatg
     4201 tgatttcata tacccgatgc caatgaaacc cagcaaggta atacctgcac tgatcaaata
     4261 tttctgctgc ggaacaggca tgaagtatga cagaatgaaa atgatgaggg gcagcaagcc
     4321 caccagaaaa aatgaaatga aagtggccag ccctatcata cccggcgatt ttgattcggg
     4381 tatcatttcc agttcttcct tcatcatcac atccacccag cgatccttgt cttcggtgat
     4441 cctggccacc acctgttcca gtagttctcc ttcaaatcct ttgttgcggt agatctgcct
     4501 gatctcttcc acctcggcat cacgcaggtg atcgatctcc cagtattcac ggcgccggtg
     4561 tttatggtac atggccagtg atgatttggc actcagaaag gcacctacag acatgctgaa
     4621 accatcggca aagagattgg caaaccccag tatcagaacg atcctcggtt ccagtccggc
     4681 accggccgca cctgccacta cggcaaaagt ggtgacactg ccgtctatcc caccatatac
     4741 aaactcggga aggtaacgac tcaggccacc ggcagaaaca ttctgttcct gatcatgatg
     4801 caatgaattt gactcttcca tagcagttgt ggtagttgct aaggtaaagg tttcaggatt
     4861 cgggaattat gacaaatact gtccttctat gggcattctt cttcccattc cgaaggcctt
     4921 cgggcttacc cgcagcaccg gagccatctg aaaacgcttc cactcattgc ggttcaccat
     4981 gcgcaaggtc tttctgacga tctcctgatc aaaacccttg tcgatgatgt cttccggact
     5041 ctggtgcttt tctatgtatt ggaataaaat ctgatcgagc agatcatagg gaggcaggct
     5101 gtcctgatcg gtctggttag gacgcagttc tgctgaaggt gctttatgaa tgatattttc
     5161 cgggatcagt tcgctgtctt tattgatata ggatgccagc tgatagacct ctgtcttata
     5221 tacatctgcc agtaccgcaa gccctccgca gaggtcgccg tacaaggtgc catagcccac
     5281 agcagcttca cttttgttag tggtattcag cagtatgtaa ccaagtttgt tgcacatggc
     5341 catcagggtc atacccctga tcctggcttg catgttctct tcggtcacat caaacgaaag
     5401 atcaccaaaa acaggatgca tctgcgccac catctgttca tacatattgt caatgggtat
     5461 gatggtatag ctgataccgt ttcttctgca caatgcttct gaatcactga cagagtgacc
     5521 actggagaag ggcgaaggca tcaggacaca gtgcacatgc tcaggcccca gtgctcttgc
     5581 tgccagcaga gctgtaacag ctgaatctat tcctccggaa agacccagta tggctttgtt
     5641 gaattgcagt tttccgaaat aatcccggat accagataac aatgcatcat gtatcagtcc
     5701 gatcttatct ttcccgttca cgttcggcgc acctgtatag ctttcagctt tttccagttc
     5761 aatgatatga agagcctctt cgaagtaggg catctccaga aatacatgtc cgcctgcact
     5821 tactgcaagg gaaccaccgt caaagatcag atctgtttgt gcacccacat ggttcacgta
     5881 gatcatgggc agtgcgtagg ccagggtatt ggctctgacc acttcaattc tgtcatgagc
     5941 ctgaccatag tcaaagggtg aggctgagat attgatcatc aggtcaggac tctgggtgcg
     6001 cagggtatcc atcgggcagc ttttatacaa tgggttctca ttgcctacat tccagatgtc
     6061 ttcgcaaact gtcagggcga ttcgcttccc tcgaaattcg accacactga actgatcatt
     6121 cggttcaaag tatctgtatt catcaaatat atcataggtg ggaagcaagg ctttattggc
     6181 ttgtccggct atctttccat cagccagcag aaacgctgca ttgaacaggt ctttaccttc
     6241 cacaacagga ttgcgtgccg ggcttcccac aatgatgccg atgtccttgc tgaaggcagc
     6301 caatcggttc actacctctt cgcatttgtc tatgaagtct tcgaactcca gaaagtcacg
     6361 aggtgggtaa ccacatactg ccagttctgg aaaaacaatg agatctgcac ctgcttcctt
     6421 cgcttcctgc acacgggcct ccatcttcga aaagttgtgt tcgaagttgc cgatgtgaaa
     6481 attaagctgg gcaagtgcaa tgcgcataaa aaaccccgga ggttggtccg gggtaaagtt
     6541 aggggaaatg acttgtttca gaacagtaca cccatgcgaa gtaccatttg tttcatctgg
     6601 atcatctcat cggtggcggc cgtttcaaat acattgacaa atccattctg gaacaccagg
     6661 cctgctgtga gggtagtagt ttctgtaatg ctgtattctg ttccgatacc catgctcaaa
     6721 gccaggttaa agaatttata ctggtcaatg gagtttttgt cgttcaggtt aacatcttgc
     6781 agctcaccgc cgtttccgtc cggcagataa ttgcctgaac catcggtcac gtcgtagcgt
     6841 gccttgatat tcatgccgag gttgaggccg aacataccat agtaggtgaa atagttgatc
     6901 tcattggtgc gcagcttgat ggtagccgga atttcaatat actgtacccg cagggtggtg
     6961 ttgatattac ccagggtggt atcttcggcg gtcagaaaac cacccagcat attgacgatc
     7021 acaccggtgc tgatggcata gcgttcaact gaaccaatgg ttggttcaaa caacaaaccg
     7081 tactggaagc ccagtctggt accgtcattt tccacatcgg taccatctac attgaaccag
     7141 gagatcaggg gcgcagcggt cagtccgaag cgaaaatcct gggcttttac tgaacccgca
     7201 aggaaaataa acaggcagga tagcagggca atttttttca tactgtatag ttttaccgga
     7261 caatgaacac cgacagattc tacaggcaca aacataaggc attttcaata atcccaattg
     7321 ttctggcagt ttccatcctg ctggtttccg ggtgcaggaa aggaccacaa ttgccccacc
     7381 cagatgtatc aggtatagaa gtggatgcca gcattcaccg gtttgatgaa gcgctgatgg
     7441 cagctgatgg ctccaactac cgggaggtca tgttggccat ggaactgcag gacagtgcat
     7501 tttttcattt gtacacaggc cagatcctga atatgcgtcc atatggcgat tctactttca
     7561 gcctgtatga tactttgtat aagtacatgg tggctgatcc gtacatgatc cgtttgtatg
     7621 atagcaccaa agctttgttt cctgatatgc gttcacagga gaaggcctta caaaaagcgc
     7681 tcaaatatta cagctattat tttcctgaaa tgcagatccc gtccttctat gcctatatcg
     7741 ctccttttgt gtatcaggtg gtggtggctg acgaggtcat cggcatcgag ctgaacatgt
     7801 tcatgggaaa ggatttcagc tactacagcg aactgtcggc caacctgccg caatatcttg
     7861 tttaccgctt cgatcccgat tacatggtgg tcagtgtgat gcgagccatg atggatggca
     7921 gcatccccaa caaaggcgct gacggcacat tgctggacga tatgctggca gaaggcaaaa
     7981 tgatgtacta tctggatctg atgttgccag atctgccgga tagcatcaag atcggattta
     8041 cagaagaaca actgaacttc tgttacgaca atgaatcaga catctggaag ttctttgccg
     8101 gagaagagct cctgttctcc accagaaatc aggacaggca aaggtatctg ggtgaatcgc
     8161 ccggagccta tggtatgccc gaaggtgccc cgggaaggat cggtatctgg gtgggatggc
     8221 agatggtcag ggcctatatg gatgcgcatc ctgaaaccaa tattcatcag ttgttgctga
     8281 tgacagaagg gctggacttt ctgcaggaaa gcggatacaa accttaattg agcacagcgg
     8341 gaacgatcat gcggaattca gggatcagca cctgaaaggt ttcgttgtca tgcgcacgtt
     8401 ccatggtata ggtgccgaac attttaccgg ccatcccccg aatgctgcag cctgatacat
     8461 actggtgcag accgcccggt tcaataagag gttgctcgcc cactacacct tcgccctcca
     8521 cttcccgcca gtcattgctg ctgtcaaaga tgtgccagtg tctgcgtaat aatctgaccg
     8581 gaaccggacc tttattttca atggtgatgc gataggcaaa gacatgttct gccgtggcca
     8641 tgccggcata gtctttctgg tagaaggtct ctacgctgac cttcacccca tcagtggtgg
     8701 cggtaaccat gtccataacg agtaaacagg ataaatgtac aaaaaatacc ttctgtcagg
     8761 acaggaatgc ggttacaata cgggccgctt tctgacaggc agtttccaga tcatcattga
     8821 tgatcacctg atcaaaacta ttgcatcgct ccagttccat ggccgctctc tgtaatctgg
     8881 ccgtcagaga ttcaggtgtt tcagtacctc tgcttcgcag tctgttctcc agcacctcca
     8941 gcgaaggcgg agcgatgaac atagacaggc tttcttccgg gtactggctc tgcagattca
     9001 gggcacccag cacatcaata tcaaagacga tacacttatc atcattccac agacgatcca
     9061 tctctgtcct gagggtacca taccatctgc ccggatacac ctcttcatac tcagcaaatg
     9121 catgctgcct gatcttttcc tgaaacgtat catgatccag aaagtagtag tcaaccccat
     9181 ctgtttcatg gtctcttttt ggccgggtag tagcagagat actgaaggcc agctgccgga
     9241 aatgcccgag cagatatcga acgatagtgg tcttgcctgc tcctgaggga gcggtaatta
     9301 tgagcaattg acggctcaca aaacgttatt caattgttct ttgatctttt ccagctcatc
     9361 tttcatgtca accaccagtt tctgcatgcc tgcatggtat gatttggcac ctatggtatt
     9421 gatctcacgc cccatctcct gactgatgaa agccagcttc ttacctttct ctatcgcttc
     9481 ctgcgtgatg ttttccagaa agtatgtgca atgtgaacga aggcgaatga tctcttctgt
     9541 aatatccatc ttttcaagat agtagatcag ttcctgctca aacctgttct tgtctatctg
     9601 ttcatcactg aactggctgt tcagttgctg catgagtttg tctttggagc gatctcttct
     9661 ttcgggatcc agttgctcta ctgcaagcag tgtatcttca atatttctgg tgcgcagttc
     9721 gaggtctttc tgcagattac ttccttcctg ctttctgaaa acggtgagct ctgcgcaggc
     9781 atcctgaagt gccttcgcca cggtgctcca ttgctcaggc agaagcgttt cctgtgtttg
     9841 tcctgctaca ttaggcattt gtgcagccag ttcaaacagc ttttcatcac ttaccagaat
     9901 ggtgcccgca gcttcccgca gacgctgcac ataggcgctg atgagtgcag gatccaggtc
     9961 tgctgaacgc tgttcttcgc cggggtcgtt gttgatcaga atatccacct ttcccctgat
    10021 aacaagtccg gatacaagat tgcgaatgtc catctcccgt tcccggaaca tattgctgag
    10081 tcttacattg atgtccagtc ctttactgtt gagtgctctt acttcaacgg tataggtctt
    10141 gccattgatg ggtagtctgg atcgaccgta gccggtcatg gataaaagca tactgccgtt
    10201 ttgtgtaaaa ttactggata ctttcctttt gcaccggttt gcgacttacc agggctacac
    10261 cggtaaagat cagcagacca gccagcacca tggtgacagt gagttgatct gtaccaagca
    10321 gcactgctac cagtgttgcc agcagtggct gcgtgtagat gtaaatactg accagggccg
    10381 ggttgcccag tttcaatcct ataatgttca taatatatac gaccagtgta gatataaata
    10441 ccacataggc ccaggaccac atgaccccgg gtgcatatcc ctgccatggc atatgcagtg
    10501 cctccggtac accaaacggc aatacccaga tcaggccgta tagaaacaac cagaaggtga
    10561 ccgtgaaggg tttgtagttg tgcatcagcg gtttggcaag aaccaggaac aaagcataac
    10621 tggccgcatt gcccatgatg agcagatcgc cgccaagggt agcttcagaa cctgcgtctc
    10681 tggctcctga ccgtatcagc atccagacac cggcggcacc tgtcagcaca ccgatagctt
    10741 tcttccaggt aagcgggtca cgcaggaaga accacgacat gatcagcacc agaatgggag
    10801 tagtgatcat gatcagggag gcgtggatct cacttgtcag gtccaggcct ttgatgaaca
    10861 tcaactgatt ggctgccaca ccgagcaggc cgcaatagaa cagcttgata tgatcttttc
    10921 tcttaactga ctgcttctcc agagtaacag ccagcaagcc gaaaatgaac aaagggatac
    10981 tcacgcgggt gagtaccagg gcaaatggcg gaatagcaga cagagccact ttggctatga
    11041 catagttcag gccgtaaatg acattggcgc caaaaatata caggtgggcc ctggtctctg
    11101 tttgcacccg gcaaaggtaa tctgattgct gtcagaaatc gaggctgagg gaggcatacc
    11161 agcgggggtc tgtaaaaccg ttcagttcaa tacccacggc ccggtcaata cgtatgaaat
    11221 aaccgaaaag ag
//
LOCUS       DAOMAX010000064        11072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_787_length_11072_cov_3.412272, whole genome shotgun sequence.
ACCESSION   DAOMAX010000064 DAOMAX010000000
VERSION     DAOMAX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_11072_cov_3.412272"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1180)
                     /locus_tag="PK742_05185"
     CDS             complement(14..1180)
                     /locus_tag="PK742_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQU76017.1"
                     /translation="MANEMYSRRSRWKLILFIIANIIIGATLFYTNNLASRLAEEELR
                     KVNQIADVYRYLATTDNIEDFGFFLDLIQANKTVPVISTDEDGNILAYQNIDSARIAT
                     HPDYLQQQLEEMKNFAQPIRFDITESSHQYIYYQHSKLYAQLRLYPIVQLVIFAAFLF
                     TAYIFFNTSRRSEQNRVWVGMAKETAHQLGTPISSMVAWIEYLKEHTSPDDSRQNVVE
                     EMDKDVKRLELIADRFSKVGSAPDLMERDLNSELRATLEYISRRASKKVQFHMQEDVS
                     PLIARVNPTLFNWVVENLLKNALDAMEGAGEISIHTNLTDKDIIVDITDTGKGIPKNK
                     QRTVFEPGYSTKKRGWGLGLTLAKRIIESYHNGRIFVKQSNAKGTTFRITLPLG"
     gene            complement(1258..3792)
                     /locus_tag="PK742_05190"
     CDS             complement(1258..3792)
                     /locus_tag="PK742_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQU76018.1"
                     /translation="MLKYKAVRISLVVLASLVLFILLTGLLVPVLFGDQIKAAFIREL
                     NKNLATEVVISEDDIQLEVLRHFPYSSVSFSNVAIRESYPGSKENFLEAGSIRLLFNT
                     VQVLRKNYTVSRIIIEDASVSLERNAGGQINYKFWKDSEGSDSEEVAIELQEVSFKNV
                     RFAYRDIPSKVFMTDDINNGKLSGNLQSDKFDLKVDARLFHRDLQVGGDHYLSDQDAH
                     VSGTILVSVPDDLYTLNNMVLEVAGNKILTNGTVTASKETLLDLDIAGQDLELEKILQ
                     LLPERYAGKIEAIASEGQLSLTAKVLGTASSASTPGIEASFNLHDGTLYHAAAKEKIR
                     RVDLQGSYSNGTLHRASSSSLQLTSLSGEYLGEPISGTLSYENFIDPTIRFDFNGRLP
                     ASVVFPFLIPGLEDISGSLLIDHFRFAGKIRAENSRRAFTEAPTGRIVMEDVAFKTGD
                     EKISLKGTLATEGIALRMDDLSLNGWGTDIQFSGTLAQWLPVIVDPQAQLAINGTLQC
                     NSIDLDRMLAVSSSPSAATAKEPAEKTAASAELWERLSGTIALRMDQFSYQDLELDNL
                     ACDLYFGRSLIAFRDIKGSTKLGKVNMNATFRRMPDRRFMLETIGMISGMDIARLFDS
                     FGNFGQDDLTSKNLKGTADIIIENANMTFDENYELQYPSVYVLAQVAINDGELIDYKP
                     LEELSGYIDVDELKHIRFNRMENQIEINKEVITIPAMEVNSSALNLYVTGTHTFDNHI
                     NYQFKIRLGEILSKKFLKRHKQDEYESSGNGVNIYVSMTGTVDHPIIEVNKKGAKDAF
                     EQEEDKGGFLDIFKPDTPLYEEPLFKKERPEETETDTLEFIEWEDD"
     gene            4032..4106
                     /locus_tag="PK742_05195"
     tRNA            4032..4106
                     /locus_tag="PK742_05195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4067..4069,aa:Val,seq:tac)
     assembly_gap    4109..4208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4781..8800
                     /locus_tag="PK742_05200"
     CDS             4781..8800
                     /locus_tag="PK742_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQU76019.1"
                     /translation="MKLVVSGLGTVKNMLVFTCIVISSIHQIALSQNGRQVRSEITDL
                     SVSNFKPESVSGSVVEYISPVYNISVEKIGKDLWYKATFSSSIESISLIFDIRFMVSA
                     GTWTDWTELNMEVQANALAIYEYSSQLSFTTIDAVAFQVRCFASDQIDPNKFITNESK
                     VILYNFEDWIVAIPSDSTISNLKSGGCFCPQPSYVTRSGWGCWDGDSPSCDPPVYTEV
                     THLIVHHEAGSNDEPVGGWDDRVFSIWYDHVFLSPEDFCDIAYNWLIDHDGIVYEGRG
                     GGDNVQGAHMCGASYNTMGVCILGNMSLIPVPIAAKSTLVDLLSWKACQEGIDPLGMS
                     YHDDPSPGTLLENIAGHSDGCADGYTECPGTYLYSDLSEIRESVNECVSGGTGAGYDI
                     SIIDVDLSDYSIYCDESFTITVTAENIGYEFMEAPNLSYYLSSDCIIGDGDEYGLGTD
                     IFADLDVGEADNETITVSMPTGYPSGSYYILALADYTDSYAEINELNNVLCAEFSYTC
                     DELPDLILTDYYLSETTVECNQEIDCFCTGWNVGGAPIDDADIAYYFSVDNVLSADDI
                     ELGTDDLPSLEPGESSDESDPLVLPSGYESGTYYILFVIDYEGVVDELDELNNVEFVE
                     IEYSCSYDIAAYFISVDEEAISGETVSGTVRFVNEGDVVIPDFSYSLSRTGGSCVYEE
                     ELDDITTAGGTVFLDLAPGEYYDASFTISIISDLDVEYIKVYADDETVYSEFDELNNF
                     DCFGISIIDPFPVDVGVTALINPTSGCDISGFYSLEFEVFNYGASPVSGVYIGYQVDG
                     GGLFALTYPGEILPGASVIISTGESFDFTGVGEHNVLVWTDMDEDIYSENDTLYAIIL
                     NIDEPVIDLGPSISACDSVILDAGNPGFDYLWSTGETSQQIVATETGYYSVTVESPGG
                     ECSSSDDVFVSISYSPSASFTYIADGLSVSFSSTSEFGDTFSWDFGDGFTSIEENPSH
                     SYVSDGTYAVVLEVSNDCGISVFSGTISVTIAGDGLPDLTGYGLTVSPSVVIPGELVT
                     VSFTMQNIGDADVVGDFISRYKVSLDCELGSDDIKIHTAEYSDLPSAGESIFYSIVDT
                     IPDTLSAGVYYIHLKIDAQTDIDESDEDNNFICSGAVVVEDSTSEELPDLLASIVSVA
                     GSIYPGSFVELGVDIYNVGLVISPFTNYSIFITDDCIGEEFQLLSTNIVSAILPGSIE
                     NYIANISIPLEWESGSSYIYVVVDEDDIVSEISESNNTDCELVNISSLLNSELGSSLN
                     IYPNPASDLLNVSATGQILLQDVTIVNNLSQAVALLNPTDEFSLQSVSIDISRIPSGI
                     YWAVIVTNQGVYKEPIIVE"
     gene            complement(9067..>11072)
                     /locus_tag="PK742_05205"
     CDS             complement(9067..>11072)
                     /locus_tag="PK742_05205"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HQU76020.1"
                     /translation="NFVLAKDVAGHIRHAESTDELRDLLSADGGGVIFTTIEKFRLKE
                     LDEGKETIHPVLSERYNIIVMADEAHRTQYGFYEGGFARNIRIALPNASFIGFTGTPV
                     DSKDADTQLVFGNTLHTYDIKQAVEDGATVPIYYEPKMVPLNIREEFLEQLEEITEGE
                     EDNTTGVWAAIEDAAGAEDRVERVAKDILTHFEARTTALEGKAMIVCMSRRNCVKMYD
                     ALTALEGCPEIAVVMTSNPGKDPAEWNHHMRTKESMEAVKKRFRNPDDPLKMVIVRDM
                     WLTGFDAPCVHTMYVDKIMKGHNLMQAIARVNRIFEDKPNGLVVDFIGISGLLAEATK
                     KYTGGGGKGDPTIDIDEAVALCLEQLARVKELMGGFEVDDIEAMTAGEKLAWSTQVIN
                     DLMVDDTTTDNFLLEERKLSELVAMTSSDSRIWDIEEDVAIIQNIRSAIRKIKYPPGP
                     GRQENERIRDLISRSLKSNAILDLAAMYDLDRIDLSIIDDKFQAIVQDKQGSNIKIEL
                     LRRIINDEIRIRTARNMKRMTNLREELEKVLGKYHQNSLDSIAAIKHLLDIAKELQED
                     DKRTKELGLAEDELAFYDLLTAHKDLLNSAGPIQELVHKVVASVKKNLQLDWTKKEDA
                     KAAIRLAVKRELRGKVPFSELDRILKEVIEQAEGQYGQWPMVA"
BASE COUNT         2711 a         2273 c         2638 g         3350 t
ORIGIN      
        1 cggaaggcat gcatcatcct aaaggcaggg tgatccggaa ggtcgtgcct ttggcgttgg
       61 actgtttcac aaagatgcgg ccattgtgat aggattcgat gatgcgtttg gccagtgtca
      121 ggcccagtcc ccagccgcgt ttcttggtgc tgtatcccgg ttcgaaaacg gtgcgctgtt
      181 tattcttggg aatgcctttt ccggtatcgg tgatatctac gataatgtct ttgtctgtca
      241 ggttggtgtg gatgctgatc tcgcccgcac cttccatagc atccagtgca tttttgagca
      301 ggttctctac gacccagttg aagagggtag gatttacacg tgctatcagc gggctcacat
      361 cttcctgcat atggaactgt acttttttac tggcacgtct gctgatgtat tccagcgtag
      421 cccgcagctc gctgttcaga tcacgctcca tcaagtcggg tgctgaccct actttggaga
      481 acctgtcggc tatgagttcc aggcgtttca cgtccttgtc catttcctcc accacgttct
      541 gccggctgtc gtcaggagag gtatgttctt tcaggtattc gatccaggcc accatggacg
      601 aaatcggtgt tcccagctgg tgggctgttt ctttcgccat tcccacccag acccgattct
      661 gttctgaacg gcgggaagtg ttgaagaaga tataagctgt gaacaggaag gcagcgaaaa
      721 tgaccagctg cacgatgggg tagaggcgca gctgtgcata gagtttactg tgctggtagt
      781 agatatactg gtgactgctt tcagtgatgt caaagcggat aggctgggcg aaattcttca
      841 tctcttccag ctgttgctgc aggtagtcag gatgggtagc aatgcgggca ctgtcaatgt
      901 tctggtaggc cagaatattc ccgtcttcgt cggttgatat aacaggaacg gtcttatttg
      961 cctgaatgag atcgaggaag aaaccaaagt cttcaatatt atcagtagtg gccaggtagc
     1021 ggtacacatc ggctatctgg ttcacctttc gcagttcttc ttcagccagt ctcgatgcca
     1081 ggttgttggt gtagaacaag gtagcgccaa tgatgatatt ggcgatgatg aacaggatca
     1141 gcttccatcg tgacctccgg ctgtacatct cattggccat gcctgaaaat tagtgattta
     1201 gtctgttagg ggatgaaaaa ccgaaggttg atctggtaat agacaaaatg ggaggtatta
     1261 gtcatcttcc cattctatga attccagtgt atcagtttca gtttcttccg gcctttcctt
     1321 tttgaacaga ggttcttcat acagaggcgt atcaggtttg aagatgtcca ggaatccacc
     1381 cttgtcttct tcctgttcaa aagcatcttt ggcgcctttc ttattgacct cgatgatggg
     1441 gtgatcaacc gtaccggtca tactcacata aatgttcacg ccattaccgg atgactcata
     1501 ttcatcctgt ttgtgccttt tcagaaactt cttactgagt atctcaccga gccggatctt
     1561 gaactggtag ttgatatgat tatcgaacgt atgggtgccg gttacataca ggttgagggc
     1621 cgaagagttg acctccatgg ccgggatggt gatgacctct ttgttgatct cgatctggtt
     1681 ttccatccga ttgaatctga tgtgtttcaa ttcatccaca tcgatgtaac ccgacagctc
     1741 ttccaggggt ttgtagtcaa tgagttcacc atcattgatg gccacctgag caagtacata
     1801 tacggaagga tactgcagtt cataattttc atcgaaggtc atgttggcgt tttcgatgat
     1861 gatatcggcg gtgcccttca ggttcttcga ggtcagatca tcctggccga aattgccgaa
     1921 actgtcgaac agccgggcaa tgtccatccc gctgatcatc ccgatggttt ccagcatgaa
     1981 acgacgatcc ggcattcggc ggaaggtagc gttcatgttc acctttccca gctttgtgct
     2041 tccttttata tcccggaagg cgatcagcga tctgccgaaa tacagatcac aggccaggtt
     2101 atcgagttca aggtcctgat agctgaattg atccatacgc agggcaattg tgccgctcag
     2161 tctttcccat aattctgcag atgcagctgt tttttctgcc ggttcttttg cagtagccgc
     2221 agatggtgag gaagaaacgg ccagcatacg atccaggtct atgctgttac attgcagggt
     2281 gccattgatt gccagttgtg cctgcgggtc cacaatgacc ggcagccatt gtgcaagggt
     2341 accggagaac tggatatcgg taccccatcc gttcagcgag agatcatcca ttcggagagc
     2401 tattccttct gtagccagag tacctttcag gctgatcttt tcatcaccgg ttttaaaagc
     2461 cacatcttcc atcacgatcc ggccggttgg cgcttcggtg aatgcccttc tggaattttc
     2521 ggcccggatc ttcccggcaa atctgaagtg atctatgagc agggaacctg agatatcttc
     2581 cagtccgggt atgagaaaag gaaatactac agaagcgggc agacggccat tgaagtcgaa
     2641 ccggatggtc gggtcaataa agttttcata ggagagcgta cctgaaattg gttcaccgag
     2701 gtattcgcca ctcaaagagg tcagttgcag actggaactg gaagccctgt gcagggtgcc
     2761 gttcgaataa gatccctgca gatctacccg acgtatcttt tctttggccg cagcgtggta
     2821 cagggtgcca tcatgcaggt tgaaacttgc ttcaataccc ggggtgctgg cagaacttgc
     2881 agtacccagt actttggctg taagactaag ctgaccttct gaggctatgg cttcgatctt
     2941 tccggcatag cgttcgggca gcaactgcag gatcttttcg agttccaggt cctgaccggc
     3001 aatatccagg tccagcaggg tttctttgga tgctgttaca gttccgttgg tcaggatctt
     3061 gtttccggcc acttccagca ccatattgtt cagggtgtac aggtcatcgg gtacagaaac
     3121 cagaatggtt ccactcacat gggcatcctg atcggagagg tagtgatcgc cacccacctg
     3181 cagatcacgg tgaaacaggc gggcatccac tttcaggtcg aatttatctg actgcaggtt
     3241 tccgctcagt ttgccgttgt tgatatcgtc ggtcataaag acctttgagg gaatatcgcg
     3301 gtaagcgaag cgaacatttt tgaatgacac ctcctgcagt tcgatggcca cttcttcgct
     3361 atcgcttcct tcactgtctt tccagaattt gtagttgatc tgtccgccgg cattccgttc
     3421 caggcttaca gaagcatctt cgatgatgat cctgcttacg gtataatttt ttcgcagcac
     3481 ctgtacggta ttgaacagga ggcggatgct tccggcttcc aggaaattct ctttggagcc
     3541 gggataactt tctctgatgg ccacgttgct gaagctgacg gaggagtagg ggaaatggcg
     3601 cagcacttcc agctggatat catcttcact gatgacgacc tcggtggcca gattcttgtt
     3661 gagttctctg ataaaggccg ctttgatctg gtcgccgaag agcaccggca ccaggaggcc
     3721 tgttagcaga atgaacagga ccagcgaggc caggacgacc agtgagatgc ggacggcttt
     3781 atacttcagc atagagcgac tgtaagatta caaaaggcca tacggtcact aaaacgtggt
     3841 gcgcacggta aattgtgaat agcgggcagg atatttggaa ataatggaca gcatattatc
     3901 tttgcactct ccttttttgg aagtccaata agagagggta gctcagttgc atgcctaacg
     3961 gcagccaggg gttcagagca tcccgcccta acaggcggga gggtcactgg ttcgactgag
     4021 aaaataaaat agggagctta gctcagttgg ttcagagcat ctgccttaca agcagagggt
     4081 cactggttcg aatccagtag ctcccacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnag agcatctgcc ttacaagcag agggtcactg gttcgaatcc agtagctccc
     4261 acagatcaaa accccgccac gatagtggtg gggttttttg ttggcagatt ttgccaagtc
     4321 tcacttgagc aaaatctgcc aacaaaaaac acagtccggc tccagccggg caggttttga
     4381 tcttagtacc cccacccacc tggatctcgc ggagcgaaat ccagtcatga gattttctac
     4441 tggatccatt ggtaactgat accatccctg ctccagccgg gcaggtttag atctccttaa
     4501 atccttaatt tctggcctta caggccaaaa aatccaggtt tattgtcttt tttcagcgat
     4561 ttcatagctg gaaccctttt ttatttgcaa acattttgca attacccgct ttgcttcata
     4621 ttgtaaacta gagtaagaag gtactctctt ttggaaatat agagtctttg tattcaaccc
     4681 tctctcagag gaattaaatc aaagtattta taaaataatt ttgaaaacat tgcttaatct
     4741 ttgaaagatt tattatttta attgataaat cttttataaa atgaaactag tggtaagcgg
     4801 attagggact gtaaaaaata tgctggtttt tacctgtatt gtaatcagtt cgattcatca
     4861 gattgcatta tctcaaaatg ggagacaagt aagatcagag ataactgatc ttagcgttag
     4921 taattttaag ccagaaagtg tatctggatc agttgtggaa tacattagtc cagtttataa
     4981 tatttcagtt gagaaaattg gaaaagattt atggtataag gcaactttta gctcttcaat
     5041 agaaagtatc agtttgatat ttgatattag attcatggta tctgcgggta cttggaccga
     5101 ttggacagaa ttaaatatgg aggtccaagc taatgctttg gcaatttatg aatatagctc
     5161 tcagctaagt ttcacgacaa ttgatgcagt tgcatttcaa gttagatgtt ttgcgtcaga
     5221 tcagattgat ccaaataagt ttattactaa tgaatcaaag gtgattctgt ataacttcga
     5281 agattggatc gtagctattc catcagactc caccataagc aatttgaaat caggaggatg
     5341 tttttgccca cagccaagtt atgtaacaag atctggatgg ggatgctggg atggcgactc
     5401 tccatcatgt gatccacctg tatataccga ggtaacacat ttaattgtac atcatgaagc
     5461 tggttcaaat gatgaacctg ttggagggtg ggatgataga gtatttagta tttggtatga
     5521 ccatgttttt ttaagcccag aagatttctg tgatattgca tataattggc taatagatca
     5581 tgatggaata gtttacgaag gtcggggtgg tggagataat gttcagggtg ctcatatgtg
     5641 tggagccagc tataacacta tgggggtttg tattttagga aatatgagtc taatccctgt
     5701 tcctattgct gcaaaatcta cacttgttga tcttttatca tggaaagctt gccaagaggg
     5761 aatagaccca ttaggtatgt catatcatga cgatccttca cctgggactt tacttgagaa
     5821 tatagctggt cacagtgacg gttgtgccga cggctatact gaatgtccag gtacatattt
     5881 atactccgat ctttctgaaa ttagagagtc tgtaaatgaa tgtgtttcgg gagggacagg
     5941 tgcaggttat gatatttcta ttattgacgt tgacttgagc gactattcga tttattgtga
     6001 tgagtctttt actataacag tcactgccga aaatattgga tatgaattta tggaagctcc
     6061 aaatttgagt tattacctct cgagtgattg tataatagga gatggagatg aatatggtct
     6121 tggtactgat atatttgcgg atttagatgt aggtgaagcg gataatgaga caattactgt
     6181 aagcatgcct actgggtacc catcagggtc ttattatatt ttggctttag cggattatac
     6241 tgattcatat gcagagataa atgagctaaa taatgtatta tgcgctgagt ttagttatac
     6301 ctgtgatgaa ctacctgact taattttaac tgattattat ttaagtgaaa ctacggtaga
     6361 gtgcaatcaa gaaattgatt gcttttgcac tggatggaat gttggaggcg ccccaattga
     6421 cgatgcagat attgcttact atttttccgt tgataacgta ctaagtgcag atgatattga
     6481 acttggtaca gatgatttgc cttcattgga acctggggaa tcttctgacg aaagtgaccc
     6541 actagttttg cctagtggtt acgaaagcgg aacatattat atactatttg ttattgatta
     6601 tgaaggagta gtagatgagt tggacgagtt gaataatgtt gagtttgtag aaatcgagta
     6661 tagttgtagc tatgatatag ctgcttattt catatctgtt gatgaggaag ctatttcagg
     6721 ggaaacggta tccggaactg taagatttgt taatgaagga gatgttgtga ttcctgattt
     6781 tagttacagt ttatcacgaa ctggtggaag ttgtgtctat gaagaagagt tggatgatat
     6841 tactacggct ggtggcactg tatttttgga tttagctccg ggggaatatt atgatgcttc
     6901 attcacaata tccataatat cagatttgga tgtagaatat attaaagtgt atgccgatga
     6961 tgagacggta tattctgaat tcgatgaact taataatttt gattgttttg gtattagtat
     7021 tatcgatcca ttccctgtgg atgttggagt gacagcgcta attaacccta ctagtggatg
     7081 tgatatttct ggcttttaca gtcttgaatt tgaagtattc aactatgggg caagcccggt
     7141 atctggtgtt tatattggtt atcaagttga cggtggtggt ctctttgccc tgacctatcc
     7201 aggggagatt ttacctgggg catctgttat tatttctaca ggtgaatcgt ttgattttac
     7261 cggcgtagga gagcacaatg tccttgtttg gaccgatatg gacgaagata tatactcgga
     7321 aaatgatact ctatacgcta taatacttaa tatagatgag ccggttattg atctagggcc
     7381 gagtatatct gcatgtgaca gtgttatttt agatgctggt aatccgggat ttgattatct
     7441 atggagtaca ggggaaacgt ctcagcaaat tgttgctaca gagactggtt attatagtgt
     7501 tacagtcgaa tcaccggggg gtgagtgttc ttcttctgat gatgtatttg tatccatttc
     7561 ctattctcca agtgcttctt ttacctacat agctgatggt ctaagtgtaa gttttagtag
     7621 tacttctgaa tttggagaca cgtttagttg ggatttcggt gatggattta catcaattga
     7681 agagaatcct tcacattcat atgtttctga tggtacttat gcagttgttt tggaagtgag
     7741 caatgattgc ggaatttctg tatttagcgg aactatttcg gtgactattg caggtgatgg
     7801 actcccagat ttaacgggat atggtttgac tgtttcaccg tcagttgtaa ttccaggaga
     7861 attggttaca gtttcattta ccatgcagaa tattggagat gctgatgttg ttggtgattt
     7921 tatttcaaga tataaggttt cacttgattg tgaacttggt tctgatgata taaaaattca
     7981 tactgcagaa tattctgatt tgccaagtgc cggggaaagt atattctata gtatagtaga
     8041 tacaatacct gacactttat ctgcaggtgt ttactatata catcttaaga ttgatgcaca
     8101 gacagacatt gatgagtctg atgaagataa taactttata tgttcaggtg cagttgtagt
     8161 tgaagatagt acttcagagg agttgccaga tttgttggca tcaattgttt ctgtcgcagg
     8221 atccatttat ccaggttcat ttgtagagtt gggtgtagat atatataatg ttggattagt
     8281 tatttctcca tttaccaatt attcaatctt tataactgat gattgtatag gggaggagtt
     8341 tcaattgtta tcgacgaata tagtttcagc aatactgcca ggaagcattg agaattacat
     8401 tgcaaacatt tcgataccct tggaatggga atctggttct tcatatatat atgtcgtagt
     8461 tgatgaagat gatattgtat cagagataag tgaatctaat aatacggact gtgaactcgt
     8521 taatatatca agtttattga attcggaact tgggtctagt ctaaatatct atcctaatcc
     8581 tgcatcagat ttattgaatg tttctgctac tggtcaaata ctacttcaag acgtaacaat
     8641 tgtaaacaat ttaagtcaag ctgtagcttt actaaaccca acagatgaat ttagtctaca
     8701 aagtgtaagc attgatatat cccgaattcc atctggtatt tattgggcag ttattgttac
     8761 aaatcaaggg gtttacaaag aaccaatcat agtagagtag tataggattg aatggactta
     8821 tgattgcaat gccgtggtaa aaatttaggg gggctcaatc gccccccctt tgcttatgtt
     8881 atttctttta ctgcttactt gcttttcctg ctttgtttta ccgctgtaag cggtgtccaa
     8941 atgcattaca gccaaggcaa taatgcaact atcagcctag ataccctgat ctttactgat
     9001 aagttgaatt taaatttcat gatgcttaaa ctatgcttgt ttgaaataag ttccaaatct
     9061 aagtcgctat gccaccatcg gccactgtcc atactgacct tcggcctgtt ctataacttc
     9121 ctttaagata cgatccaatt ctgaaaaggg cactttacct cgtaattccc gcttaacggc
     9181 caggcgaatg gctgctttgg catcctcttt cttggtccag tccagttgca ggttcttttt
     9241 gacggaggct actaccttgt gtacaagctc ctggatgggg cctgctgagt tgagcaggtc
     9301 cttgtgggct gtgaggaggt cgtagaaggc tagttcgtct tctgctaggc ctaattcttt
     9361 ggtacgttta tcatcctctt gcagttcttt agcgatatcc agcaggtgct tgatggctgc
     9421 tatggaatcc aggctgttct ggtggtactt accaagcacc ttttccagct cttcccggag
     9481 gttggtcatc cgtttcatgt tcctggcagt gcggatgcgg atctcatcgt tgatgatgcg
     9541 cctgagcagc tctattttga tgttggagcc ttgcttatcc tgcacaatgg cctggaactt
     9601 atcgtcaatg atggagagat ctatgcggtc cagatcatac atggctgcca gatccaggat
     9661 ggcattggat ttcagtgatc tgctgatcag gtctctgatg cgctcatttt cctgcctacc
     9721 gggaccagga gggtatttga tcttccggat ggcggagcgg atgttctgaa ttatggctac
     9781 gtcttcttcg atatcccaga tacggctatc ggaactggtc atggccacca gctcactcag
     9841 tttgcgctct tccagtaaaa agttatcggt ggtggtatca tctaccatca ggtcattgat
     9901 cacctgggtg ctccacgcca gcttttctcc tgcggtcatt gcttcgatat catccacctc
     9961 aaagcccccc atcaactcct tgaccctggc cagttgttcc aggcacaggg caacagcttc
    10021 atcaatatca atagtaggat ctccttttcc gccacctccg gtgtatttct tggtggcctc
    10081 tgccagcaga ccggagatgc ctataaagtc caccaccagg ccattgggtt tgtcttcaaa
    10141 gatccggttc accctggcaa tggcttgcat caggttgtgg cccttcatga ttttgtccac
    10201 gtacatagta tgtacgcagg gggcatcgaa gccggtcagc cacatatccc gcacgatgac
    10261 cattttcaga ggatcatccg gattgcggaa tcgcttctta acagcctcca tggattcttt
    10321 ggtgcgcatg tggtggttcc actctgccgg gtccttgccg ggattgctgg tcatgaccac
    10381 ggctatctcc gggcaccctt ccagggcggt gagcgcatca tacattttca cgcagttgcg
    10441 gcgactcata cacacgatca tagccttccc ttccagggct gtggttcggg cctcaaaatg
    10501 tgtcagaatg tctttggcca ccctttctac ccggtcttcg gctccggcag catcttcaat
    10561 ggctgcccag acacccgtag tattgtcttc ttcaccctcg gtgatctctt ccagctgttc
    10621 caggaattct tccctgatgt tcagcggtac cattttgggc tcatagtaga tgggcactgt
    10681 ggcaccgtct tcaaccgctt gtttgatatc gtaggtatgc agggtgttgc caaataccag
    10741 ctgcgtatca gcatccttgc tatccacagg ggtaccggta aagccgataa aggaagcatt
    10801 tggcagggcg atgcggatat tccgggcaaa gccgccttca tagaagccat attgggtgcg
    10861 atgggcttca tcggccatca caatgatgtt gtagcgttcc gacaacaccg gatggatggt
    10921 ctctttgcct tcatccagct ctttcagacg aaacttctca atggtagtga agatgacacc
    10981 ccctccatca gctgaaagca ggtcacgcag ctcgtcagtg ctctccgcat gcctgatatg
    11041 acctgctaca tctttggcca ggacgaagtt tt
//
LOCUS       DAOMAX010000065        10922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_813_length_10922_cov_4.145486, whole genome shotgun sequence.
ACCESSION   DAOMAX010000065 DAOMAX010000000
VERSION     DAOMAX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_813_length_10922_cov_4.145486"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..878
                     /locus_tag="PK742_05210"
     CDS             63..878
                     /locus_tag="PK742_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQU76021.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLAASLAVLEFKTLLIDADPQAN
                     STSGNGFDPKENRPNIYDCMINEKKASDAIIKTETPNLYLLPAHLDLVGAEIELINHP
                     NRERIMGAALEDIKNEFDFIFIDCSPSLGLITVNALTAADAVLVPVQCEYFALEGLGK
                     LLNTIKIVQNRLNENLQIEGILLTMYDARLRLSNQVVTEVKRHFQTLVFNTIIHRNTR
                     LGEAPSFGKPAIMYDAECTGAINYLNLAREILQNNKLAGVHSEDEGIEESLEG"
     gene            892..1779
                     /locus_tag="PK742_05215"
     CDS             892..1779
                     /locus_tag="PK742_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQU76022.1"
                     /translation="MSKQKQDLGKGIRALLENMDQEAGADFVQKKKAEMSHSSTIPLE
                     AIEINPFQPRVEFQEEALSDLADSIRVHGVIQPVTVRRLESGRFQLISGERRLRASKL
                     AGLKEIPAFVRDANDQEMLEIALIENIQREDLNAIEISINFKRLLDECNLTHEQLAGR
                     LGKNRTTVTNFLRLLKLPPDIQTGLRDKSISMGHARAILSLEDPVAQLDVFREVIKQG
                     MSVREVEAFIKQYQQGGKKAGGSKPVQVPVHVRKVQEELSSHLSTRVVIKQDKSGKGQ
                     ITISFFSDDDLYRIKDLLD"
     gene            1806..2384
                     /locus_tag="PK742_05220"
     CDS             1806..2384
                     /locus_tag="PK742_05220"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HQU76023.1"
                     /translation="MAIAILFAGNCLSQTDSLIAPTEIRLNAVTEETLHSPKKAALYS
                     ALLPGLGQAYNQKYWKIPVVYTALGISGYSIWFNARYYSDLKNAYLIRTDGDSTTIDQ
                     YANILPVESQLIQYAEFYKKSMDLSVIITAAIWTLNIVDAVVDAHLYNFDISDDLSMQ
                     IKPAFRREQQIGGNGVQTWNSVYGVQIKLSIR"
     gene            2381..3136
                     /gene="dapB"
                     /locus_tag="PK742_05225"
     CDS             2381..3136
                     /gene="dapB"
                     /locus_tag="PK742_05225"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQU76024.1"
                     /translation="MKVALIGYGKMGRSVESVIEEYNSKGPEIPVEVALKIDLENMDT
                     FTPDSLQGIDVAIEFTNPESAVGNIKKCLDSGVAVVCGTTGWYQHVEEVKAYCQERNG
                     TLLYTPNFSVGVNILFELNEKLAAIMSSRTGYTVTITELHHKEKLDAPSGTAINLAEQ
                     IIQANPGISKWTLGKSDDETTISIMSVRETGVPGTHVIHYEADEDEITVIHKAHNRKG
                     FATGAFNAALWVKGRTGIFTMKDVLDLNAGSRF"
     gene            complement(3152..5668)
                     /locus_tag="PK742_05230"
     CDS             complement(3152..5668)
                     /locus_tag="PK742_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQU76025.1"
                     /translation="MRKLFAAFFVLVPLFSIAQTPEKPNIIFIMTDDMNDYVEGFDGH
                     PQAETPNISSLADMGTIFYNAYSNSVLCAPSRTSFMSGKNPEYTSVMINGLYNCDFRS
                     NFPPGLPIYTFPQILKDSAGYFTVSVGKLFHCDDGEVDYDLGNPDPCTRQLSWNKTYQ
                     KHLSPSVNDAGQANPQGIDEIKWSKIDSFLVDSMPDFEAIDSTIYFLQQYAANPGDFC
                     DKPFMIALGIQKPHSHVYIPEQYFSDDYIDDFLAEPFDINYNYPKGSYPYNGLVMPAL
                     TDPPYMDMDSLGPLALEQIPLAPYNEFANWYITGLPYIPEIDPGLTDEERYEILVEAK
                     KANGIMAYMAAIRFVDDMVGKLMDTLANYPDLYNNTIIVFSSDHGYSWGEKRMYGKSQ
                     MWETDSRVPFIIADLRNPNPQFCTKSVGLVDLFPTLMEMAGIEQPKNPDGTDYPDGHS
                     LIPLMEDPDRQWEYPVLTQVKNNFYGNVCWPQNSIKNNRFHLIQFRSNGEGTTSVCDL
                     PNSWVENYLYEVGEERNVDPEEWYNLAEKPEYQPVIQYLEEFLPGGSMYTEKAFTVKV
                     FDKSPKCLYGSSDVIQMKAVVYNENGSQLLGPALADYQVIWSNSLTGETSTGYTYSFD
                     LSTLTPSQFLENDKILFYAHVVDTDSGHVRGMEVKYAYINPGNKPVASFDVSMDGLTA
                     TVDPYTLTGSYTSSLWNFGDGHTTSEFVPGPYTYPAAGSYNIRNIVYYGNGCKRTFKK
                     PVVATMLAGAENERSAGAFRMAIIPNPANEFCSLQFNETVSQGIIRVYDMTGKMVMEQ
                     TVPEGNTFVSLMTWKLAAGLYQIELNSTNGDLQTSLQVNH"
     gene            5763..7184
                     /gene="dacB"
                     /locus_tag="PK742_05235"
     CDS             5763..7184
                     /gene="dacB"
                     /locus_tag="PK742_05235"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HQU76026.1"
                     /translation="MKYGILVAFFFGIAELVNAQNTVGQLLAQWEMEPALKTATWSFN
                     MVRVEDGNTIASRNPDTCLPLASGMKAITTLAAYEVLGKDFRWETTLKTDGILTDSIL
                     NGNLILHGYGDPTLGSSRITGSDRFDTLFRSWAATLYASGIRVIQGRILTDLSSMDND
                     PVPGSWNWDDIGQYYGAGCYATNIFENTYTLYYSSTSSKTRIDSIFPTIDGMEVTNDV
                     TVSGSRDNAFIYGAPGDLHRRVTGYIPANRKAFEVDGSMPDPPAFAVQELKKALTDAG
                     IEIRQSSMTEKAQTKVLISHQSPVLDSVIWHTNRKSINLYAESILKTMAWKQTGTGSY
                     TSGFKAVRSVLSKTGANMDGFNMEDGSGLSRLNAVSSAQMTAFLSYAYRQPFGTSYLE
                     SLSVSGVSGTLKNITGGTVAEGKVFAKSGSMQKVRSYSGFVQASDGKLYAFCIIVNNY
                     NCSSSVIRKKLEDLMVAMVRYAP"
     gene            complement(7172..7534)
                     /locus_tag="PK742_05240"
     CDS             complement(7172..7534)
                     /locus_tag="PK742_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76027.1"
                     /translation="MDKLVMVYNADGGWENAIMDSLHKIFSPSTYPCKLCAVTFGLRK
                     MDKTWEDWLNKQPMEKVFLHKDEWPSSGYDQSITLPAILLDSGSSAKVLVSAAEWNDI
                     PDAATLIRVLEMKLTQGA"
     gene            complement(7534..7905)
                     /locus_tag="PK742_05245"
     CDS             complement(7534..7905)
                     /locus_tag="PK742_05245"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQU76028.1"
                     /translation="MSSRTQLNRALRLKCPRCGESDLFKDPNPFNLGHLSEMHRQCPH
                     CKLKIEPETGFYYGAMWISYAIGVFASLILIGLLILAFHVDTLWAFIAVVVFHIVFSP
                     YLFRLARAVWLSFYVHQHRDI"
     gene            7951..10170
                     /locus_tag="PK742_05250"
     CDS             7951..10170
                     /locus_tag="PK742_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76029.1"
                     /translation="MHTRLIAWCWLIPLSAGAQPFIASYEHNDLEKCIQQCDKEIDKG
                     GDKQLAYLYKSKAYHRKYSADNSCLTCLTKSMNSLEMLAVKDADGVFRQGRQADTDSI
                     LTDMRAEVREAILSGPSWFVDDLLPRMEKFSDHYYEKYLRGLQATLDDDLYQATKWYN
                     EAARQIYLEWKNGKQPADESLEVFSALGDGLIDFNDHRSAITVWQRALKMYPRQEVLP
                     AYESFLNQASHNSLVTQNDSMLDIYLQAYDSLAIAFPELQVNDMLTGLLLYADEYRDT
                     PVNFIAGLVCARYPALMPVISDSLMQHWESSVAFTVVRGKLRTAWDQEAVENIIQLST
                     CAGDAGFEGKPPAHRFHQLVSSDPVMAAYWYYCLTEHGQNLSEWNADLSLLTGKLNDM
                     LEEQFTPELYTARLALPADKKDALELLDISLRMIKEQIDQGDFSGSGEWLRGELSRDP
                     ENEQLRALYKYWVTQDYLLNLEAPQSRRSHLQWKGDPDKCDAGTLPEEAYTDFMQAMN
                     YLRRLAGVPDSAVLLPEWNEKCQAAALMMHAEGHLSHQPGSNFQCFSKEGQQAAGMSN
                     LSLGYGGIPALLGQVYDSGSSNGSVGHRRWVLTPYRRLYGMGSTDDAMALWTLGGRDA
                     NFDRNITDQYTDRFVAWPPEWYFPAELMPYRWSFSMGFADFSSTTIRVFHGKKEIPVH
                     VLPLEQGYGQNTIVWEIFDLPPLDIVEQSFRVEVRHVLVNSQPMDYTYEVISLPIME"
     gene            10201..10671
                     /locus_tag="PK742_05255"
     CDS             10201..10671
                     /locus_tag="PK742_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76030.1"
                     /translation="MSLALILIIIALGLGFLFVEVFLIPGTSVLAILGFVVIIIGVYL
                     GYREFGQTTGNLLTVFSIVGAGLTMYIGYKRVQSKKWGLHTVVDGKVNVHDFSEYEVG
                     EEGVAASDIRPEGKASFSNDRRVNVYSMGEFIDAGTAIGIVRIQHNKIYVKPIV"
     gene            10673..>10922
                     /locus_tag="PK742_05260"
     CDS             10673..>10922
                     /locus_tag="PK742_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76031.1"
                     /translation="MPEQPVYQLLVGLIGIVLLYLIFSFLKYYRPWLSARISGVRIGI
                     IELYRLQWIRIDPVKFLDTLILVEKAELPVNREQIKQAY"
BASE COUNT         2988 a         2492 c         2664 g         2778 t
ORIGIN      
        1 ttttgagaac gaagataaaa ccttatacct cctgccaata ggagaaacaa ccaactttta
       61 atatgggaaa gatcatagca atagccaatc aaaaaggtgg agtaggcaaa actaccacgg
      121 ccattaatct ggcagccagc ctggccgtac tggagttcaa gaccttgctg atagatgctg
      181 atccgcaggc aaacagcacc tcaggaaacg gttttgatcc gaaagagaac cggcctaata
      241 tctatgattg catgatcaat gaaaaaaagg cgtctgatgc gatcattaaa acggaaacac
      301 ccaacctgta tttgttgccg gctcacctgg acctggtagg tgcagaaatt gaactcatca
      361 accacccgaa cagagaacgc atcatgggtg ctgctctgga agacatcaaa aatgaattcg
      421 actttatatt tatagattgt tcaccttcac tggggctcat tactgtgaac gcactgactg
      481 ccgcagatgc agtgctggtg ccggtacagt gtgagtattt tgccctggaa ggactgggga
      541 aactgcttaa taccattaag atagttcaga accgactcaa tgagaacctg cagatcgaag
      601 gtattctgct caccatgtat gatgcacgac tgcgtttatc caaccaggtg gtaacagaag
      661 tgaaaaggca ttttcaaacc cttgttttca acaccataat ccatcgaaat accaggctcg
      721 gtgaagcacc cagttttgga aagccggcaa tcatgtatga tgcagaatgt accggagcta
      781 tcaattatct gaacctggcc cgtgagatcc tgcagaataa caaactggca ggcgttcatt
      841 ctgaagacga gggcatagaa gaatctctgg aaggataagc atgcagtaac catgagcaag
      901 cagaaacaag acctgggtaa aggcattcgg gcgctccttg aaaatatgga tcaggaggca
      961 ggtgctgact ttgtccagaa gaaaaaggcg gagatgtcgc attccagtac cattccactg
     1021 gaggctattg agatcaatcc atttcaaccc agggttgaat ttcaggaaga ggcacttagt
     1081 gatctggctg attctattcg tgtacacggt gtcatacagc cagtaaccgt cagaagactg
     1141 gaaagtggac gctttcagct catctccggc gaacgcagac ttagagccag taaactggca
     1201 gggctaaaag aaattcctgc atttgttcgg gatgccaacg atcaggagat gctggagatc
     1261 gctcttattg agaatattca gcgggaagac ctgaatgcta tagagatctc catcaatttc
     1321 aagagactgc tggatgagtg caacctgacc cacgaacaac tggccggtcg cctcggcaag
     1381 aacaggacca cggtaaccaa cttcctcaga ctgctgaagc ttccacctga tatccagaca
     1441 ggtttaagag acaaatccat cagtatgggg catgcacgtg ctattctaag cctggaagat
     1501 ccggtggcgc aactggatgt tttcagggaa gtgatcaagc agggtatgtc cgttagagaa
     1561 gtagaggcat ttatcaaaca gtatcagcaa ggtggaaaaa aggcaggtgg aagcaagcct
     1621 gtacaggttc ctgtgcatgt gcgtaaggta caggaagaac tctcttctca cctgtccaca
     1681 cgggttgtga tcaagcagga taaatcagga aaagggcaga tcaccatctc tttcttttca
     1741 gatgatgatc tttacaggat caaagatctt ctggattgac cagcagcata cgagtacttt
     1801 cttgcgtggc aatagccata ctgtttgcag gcaactgcct gtctcagaca gattctctta
     1861 ttgctccaac ggaaatacgc ttgaatgcag taacagaaga aacgctgcat tcgccaaaaa
     1921 aggccgcttt atactcggct ctgcttcccg gactggggca ggcatataat cagaagtact
     1981 ggaagatccc ggtggtctat accgctttag gaatttcagg ttattccatc tggttcaatg
     2041 cccgctacta cagtgacctg aaaaatgcat acctgatcag aactgatggc gattccacta
     2101 ccatcgatca gtatgcaaat atccttcccg tagaatcaca actgatccag tatgctgaat
     2161 tctacaagaa aagcatggac ctgtctgtca ttataacggc agccatctgg acactgaata
     2221 ttgtggacgc agtggttgat gcccatttgt ataactttga catctcagat gacctgtcca
     2281 tgcagatcaa gcctgccttt cgccgtgaac aacagatagg aggtaatggc gttcagacct
     2341 ggaacagtgt gtatggggtg cagataaaac tcagtatcag atgaaagttg ctttaatagg
     2401 atatggaaaa atgggccgtt cagtagaatc ggtcattgaa gaatacaaca gcaaagggcc
     2461 tgaaattcct gtggaagtgg cgctgaaaat agatctggag aatatggaca catttacccc
     2521 agacagcctt cagggaattg atgtggccat tgaatttacc aacccggaat ctgcagttgg
     2581 caacatcaaa aaatgccttg attcgggtgt ggcagtggtc tgtggcacca ccggatggta
     2641 tcagcatgta gaagaggtga aagcctattg ccaggaaaga aacggaacct tgctatacac
     2701 accgaacttc agcgtcgggg tcaatattct ttttgaactc aatgaaaagc tggcagctat
     2761 catgtccagc cgtacaggat atactgttac aataacagaa ttacaccata aggaaaagct
     2821 ggatgcccca agcggcacgg ccattaacct ggctgaacag atcattcagg ccaaccccgg
     2881 aataagtaaa tggacactcg ggaaatcaga cgatgaaacc accatctcta tcatgtctgt
     2941 cagagagaca ggtgttcccg gaacacatgt gatccattat gaagccgatg aagatgaaat
     3001 aaccgtcatt cataaagcgc ataacagaaa aggctttgct accggggcat tcaatgcagc
     3061 cctctgggtc aaagggcgca caggcatctt tactatgaaa gatgtgctgg accttaatgc
     3121 tggaagccgg ttttaaggct ggtgcctcct gtcagtgatt cacctgcaag gatgtttgca
     3181 gatcaccatt agtgctgttc agctcaatct ggtataagcc ggcagccagt ttccaggtca
     3241 tcagacttac aaatgtgttg ccttccggaa cagtttgctc catgaccatt ttccctgtca
     3301 tgtcatacac cctgatgatg ccttgtgaaa ctgtttcatt gaattgcagt gaacagaatt
     3361 cattggcagg gttaggaata atagccatgc ggaatgcccc agcagaacgt tcattttctg
     3421 caccggccag catcgtggct accacaggtt tcttgaacgt gcgcttacaa ccgttaccgt
     3481 aatatacaat attgcggatg ttgtaactgc cggcagccgg atatgtgtat gggccgggaa
     3541 caaattctga agttgtgtgg ccgtcgccaa agttccatag actggaagta taacttccgg
     3601 tcagggtata aggatcaacc gttgcggtaa gaccgtccat gctgacatcg aaagaagcca
     3661 ccggtttgtt acccgggttg atataggcat acttgacttc cattcctctc acgtgtccac
     3721 tgtcagtatc taccacatga gcatagaaga ggatcttgtc attttccaga aactgtgagg
     3781 gcgttaatgt ggagaggtcg aaactataag tatatccggt actggtttct ccggtcaaac
     3841 tgttactcca gatcacctgg taatctgcaa gagccggtcc aagaagctga ctaccgttct
     3901 cattgtacac aactgctttc atttgaatga catcgctgct gccatacaaa cacttcggac
     3961 tcttatcaaa taccttaact gtgaatgcct tttcggtgta catggatccg ccgggcagaa
     4021 actcttccag gtactgaatg accggctggt attcaggttt ttctgccagg ttgtaccatt
     4081 cttccggatc tacatttcgc tcttcaccaa cttcatagag atagttctct acccaggaat
     4141 tgggcaggtc acaaactgat gtagtgccct ctccgttgct tctgaattgt atcagatgaa
     4201 atctgttatt cttgatgctg ttctgaggcc agcaaacatt accgtaaaaa ttattcttca
     4261 cctgggtcag aacaggatat tcccattggc gatcagggtc ttccatcaac ggtatcaggc
     4321 tgtgtccgtc cggatagtcg gtaccatcag gatttttagg ctgttcaatt cccgccattt
     4381 ccatcagtgt gggaaagagg tccaccagac ctactgattt ggtacaaaac tgaggattcg
     4441 gatttcttag atctgctatt atgaaaggaa cacgggaatc agtttcccac atttgagatt
     4501 ttccatacat tctcttttcc ccccaggaat atccgtgatc actcgagaaa acaataatgg
     4561 tgttgttata taggtccgga taatttgcca gggtatccat tagtttgccc accatgtcat
     4621 ctacgaaccg gatggcagcc atataggcca tgataccatt cgctttctta gcttccacca
     4681 ggatctcata acgctcttca tctgtcagac cagggtctat ttccggtata taaggcaggc
     4741 cggttatata ccagttggca aactcattgt aaggagccag cggtatctgt tccaatgcta
     4801 gaggcccgag actgtccatg tccatatatg gaggatctgt caatgccggc atgactagtc
     4861 cgttgtaagg ataggagcct tttgggtaat tatagttgat atcaaaaggc tcagccagga
     4921 aatcatctat atagtcatca gaaaagtatt gttcagggat gtacacgtgt gagtgcggct
     4981 tctgaatgcc caatgcgatc ataaatggtt tatcgcagaa atctcccgga ttggcagcat
     5041 attgctgcag gaaatagatg gtgctgtcta tggcttcgaa atccggcatg ctgtccacca
     5101 gaaaactgtc tatcttgctc catttgatct catcaattcc ctgcgggtta gcttgtccgg
     5161 catcattcac agatggagat agatgctttt gataggtctt gttccagctg agttggcggg
     5221 tgcatgggtc cggattgcca aggtcataat caacttctcc gtcatcacaa tgaaacagct
     5281 ttccaacgga aacagtgaag taacctgcac tgtctttcag tatctgcgga aacgtataaa
     5341 tcggtaaacc gggagggaaa ttgcttctga agtcgcaatt atacaatccg tttatcatga
     5401 cgctggtgta ttccggattt ttaccggaca taaagctcgt gcgtgacggg gcacaaagca
     5461 cagaattgct gtatgcattg taaaagatag tccccatgtc agcaaggctt gaaatgttgg
     5521 gtgtttcagc ctgcggatga ccatcaaaac cttcaacata atcattcatg tcatcggtca
     5581 tgataaaaat gatattgggt ttttcagggg tctgggcaat tgaaaacaat gggaccagta
     5641 caaaaaacgc ggcgaataat ttcctcataa ggaaccttta atggcactca aaggtacaaa
     5701 actgactttt ttccaccggc tttgccttca atatattctg catgtttgcc gttctttacc
     5761 acatgaaata cgggatttta gttgccttct tcttcggtat cgcagagtta gtcaatgccc
     5821 aaaacactgt cggtcagtta cttgctcagt gggagatgga gccggcgctg aaaacggcca
     5881 catggagttt taacatggtc agagtggaag acggaaatac catcgccagt cgaaatccgg
     5941 acacttgtct tcctcttgcc agcggcatga aagctatcac aacactggca gcttatgaag
     6001 tgctgggaaa ggattttcgc tgggaaacta cgctgaaaac tgacggaatc ctgacagata
     6061 gtatcctgaa tggaaacctg attcttcatg gttatggcga tccaacatta ggcagcagca
     6121 gaataaccgg ctccgatcgt tttgatacac ttttcagatc ctgggctgct acactgtacg
     6181 ccagtggtat tcgcgtaata cagggcagga ttctgacaga tctgtcatca atggataatg
     6241 atccggttcc cggcagctgg aactgggatg atataggtca gtactacggt gcaggttgtt
     6301 atgcaacgaa tatatttgag aacacctaca cactgtacta ttccagtact tcttcaaaga
     6361 caaggatcga cagcattttt ccaaccatag acggtatgga ggtgaccaat gacgtgaccg
     6421 tttcaggcag tcgagataat gcctttatct atggtgcgcc aggagatctg cacagaaggg
     6481 tgaccggtta tattcctgcc aacagaaaag catttgaagt ggatggctcc atgcctgatc
     6541 caccagcatt tgctgtacag gagttgaaaa aagcactgac tgatgcgggt atcgagatcc
     6601 gtcagtcatc catgacagaa aaggcccaga ccaaagtact tatcagccat cagtcgccgg
     6661 tacttgacag cgtgatctgg cataccaaca ggaagagcat caatctctat gccgagtcta
     6721 ttttaaagac aatggcatgg aagcaaaccg gcaccggcag ttatacaagc gggttcaaag
     6781 ctgtcagatc cgtgctgtca aaaacaggag ctaacatgga cggattcaac atggaagacg
     6841 gcagcggact ctccagactc aacgctgtaa gcagtgcaca gatgaccgca tttttatctt
     6901 atgcctaccg gcagcctttc ggcacgtctt atctggaatc actcagtgtg tcaggcgttt
     6961 caggaacgct caagaatata acaggtggca ctgtagccga agggaaagtg tttgccaaaa
     7021 gcggatctat gcaaaaggtg cgcagctact cgggctttgt acaagcttct gacggcaagc
     7081 tctatgcttt ctgcatcatc gtcaacaact acaactgcag ttcttcggtg atcagaaaaa
     7141 aactggaaga cctgatggtg gccatggtgc gctatgctcc ctgagtgagc ttcatttcca
     7201 gcaccctgat cagagttgca gcatccggta tgtcattcca ttcggcggcg cttacaagta
     7261 ctttggctga tgagcctgaa tcaagcaata ttgcaggtaa tgtgatactc tggtcgtaac
     7321 ccgatgaagg ccattcatcc ttgtgcagga acaccttctc cattggttgt ttgttaagcc
     7381 agtcttccca tgttttatcc atctttctca ggccaaatgt taccgcacaa agcttacacg
     7441 gataagtaga aggtgaaaag atcttatgca aagaatccat aatggcattt tcccagccgc
     7501 catcggcatt gtaaaccatc accagtttgt ccatcagatg tcgcggtgtt ggtgcacata
     7561 gaaacttagc cacaccgctc tggccagcct gaacaggtag gggctgaaca caatatgaaa
     7621 gaccactact gcaataaatg cccagagtgt gtccacatga aatgccagta tcagcagacc
     7681 gatcaggatg agactggcaa acaccccgat ggcatagctg atccacatgg ctccgtaata
     7741 gaatccggtt tctggttcta tcttcagttt gcaatgcgga cattgcctgt gcatctctga
     7801 caggtggccc agattgaaag ggttgggatc tttgaacaga tcactttcac cacaacgtgg
     7861 acatttgagc cgaagggccc ggttgagctg ggtacgggaa gacatgatct gcaaaaatac
     7921 ttttattgtg aaccaatcgt ttgtgaccat atgcacacac gcctgatagc ctggtgctgg
     7981 ctgatccccc tgtcggccgg tgcacagcca ttcattgctt cctatgagca caatgacctg
     8041 gaaaaatgca tccagcagtg cgacaaggag atcgataaag ggggcgataa acaactggct
     8101 tacctctata agtctaaggc ataccataga aagtattcag ctgacaatag ttgtcttacc
     8161 tgcctgacca agtccatgaa cagcctggaa atgctggctg tcaaagatgc cgatggtgtc
     8221 ttccgtcaag gtcgccaggc ggatacagac tccatactaa cagacatgcg tgcagaggtc
     8281 agagaagcta tcctgtcagg accttcctgg ttcgtggatg acctgcttcc caggatggag
     8341 aaattctccg accattacta tgagaaatat ctgcgtgggc tgcaggccac tctggatgat
     8401 gatctgtacc aggcaaccaa atggtacaat gaggctgcca gacagatcta tctcgaatgg
     8461 aagaacggga agcaaccggc agatgaatcg ctggaggtgt tctctgcact gggcgatgga
     8521 ttgatcgatt ttaatgatca tcgttcggcc attacggtct ggcaaagagc attaaaaatg
     8581 tatccccgac aggaggtgct cccggcttat gagtcatttc tgaaccaggc gtcacataac
     8641 tctcttgtta cccagaacga cagtatgctg gatatatacc tgcaggccta tgattcactt
     8701 gcaatagcat ttccggagct tcaggtgaac gatatgctaa ctggtctttt gctttatgca
     8761 gatgagtatc gcgatacacc tgtcaacttc attgcgggtc ttgtttgtgc aaggtatccg
     8821 gctctgatgc ctgtcatcag tgactcgctg atgcagcatt gggaaagctc agtggctttc
     8881 acagtggtaa gagggaagct tcgaaccgcc tgggatcagg aagctgtgga aaacatcatt
     8941 caactgtcaa cctgtgcggg tgatgcagga tttgaaggaa aacctcctgc acatcgtttt
     9001 catcagctgg taagttctga cccggtgatg gctgcttact ggtattattg tcttacggag
     9061 catggtcaaa acttgtcaga atggaatgca gatctttccc tgcttactgg taagctgaat
     9121 gatatgctgg aagaacaatt cactccggaa ctctatacag caagacttgc tctgcctgcc
     9181 gataagaaag acgcattgga actgcttgat atctcactca gaatgataaa ggagcagatc
     9241 gaccagggag atttttctgg ctctggtgaa tggctgcggg gtgaactcag ccgggatcct
     9301 gagaacgagc aactccgggc gctgtataag tactgggtca cgcaagacta tctgcttaac
     9361 ctcgaagctc cacaaagcag acgatcacat cttcagtgga agggtgaccc ggataagtgt
     9421 gatgcaggta cattgccgga agaggcatac actgatttca tgcaggccat gaactatctg
     9481 cgcaggcttg ccggtgtgcc ggacagtgcc gtattactgc ccgaatggaa tgaaaaatgc
     9541 caggcagcag ctctgatgat gcatgcggaa gggcatctga gtcaccagcc cggcagcaac
     9601 tttcagtgct tttcaaaaga ggggcaacag gcagcaggca tgtctaattt atcattggga
     9661 tatggaggta ttcctgcttt gctgggccag gtatatgaca gcggaagcag caatggctct
     9721 gtcggacaca gaagatgggt gctgacccct tacagaaggc tgtacggtat gggcagtacc
     9781 gatgatgcca tggctttgtg gaccctgggg ggaagagacg ccaactttga ccgtaacatc
     9841 acagatcagt acacagaccg cttcgtagcc tggccaccgg aatggtactt tccggctgag
     9901 ttgatgccat accgatggtc gttttctatg ggtttcgccg atttctcatc caccaccatc
     9961 cgtgtgtttc atggaaaaaa agagatcccg gtccatgttc ttcccctgga acaaggctat
    10021 ggtcagaata ccatcgtgtg ggagatcttt gatctgccgc cacttgatat tgtggaacag
    10081 tcttttaggg ttgaggttag gcatgtattg gtaaacagcc agccgatgga ctatacctat
    10141 gaggtgattt ctttgccaat aatggaatga tgtaatgcca tccccgtata tttacggtcc
    10201 atgagtcttg cactgatcct cattatcatc gccctggggc tgggattcct atttgtagag
    10261 gtcttcctca ttcccggaac ttctgtactg gccatcctgg gctttgtggt cattatcatt
    10321 ggcgtatatc tgggctatcg cgaattcggg caaaccaccg gcaatctgct gacggtcttc
    10381 agcattgtgg gtgccggttt gaccatgtat atagggtaca aacgggtgca atcaaaaaaa
    10441 tggggactgc ataccgtggt ggatgggaaa gtcaatgtac acgacttcag cgagtacgaa
    10501 gtgggggaag aaggcgtagc agcatctgat atccgtcccg aaggcaaagc cagtttcagc
    10561 aatgaccgtc gggtgaatgt gtacagcatg ggcgaattca ttgatgccgg aacggccatt
    10621 gggatcgttc ggatacagca taacaaaata tatgtgaaac ccatcgtctg atatgcctga
    10681 acagccagta tatcaactat tagtcgggct gatcggtatt gtcttactct atctgatatt
    10741 ttcattcctg aaatattatc gtccgtggct ttctgcccga atttctgggg tgcgtattgg
    10801 aatcatagaa ctttatcgtc tgcagtggat cagaattgac ccggtcaaat tccttgatac
    10861 actgattctt gtagaaaaag cggagcttcc tgtgaataga gagcaaataa aacaagcata
    10921 tc
//
LOCUS       DAOMAX010000066        10907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_817_length_10907_cov_3.547272, whole genome shotgun sequence.
ACCESSION   DAOMAX010000066 DAOMAX010000000
VERSION     DAOMAX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_10907_cov_3.547272"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            442..1155
                     /locus_tag="PK742_05265"
     CDS             442..1155
                     /locus_tag="PK742_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76032.1"
                     /translation="MDWEWYRDGNTMRVWMHLLLKAEYQDREYRGVVIRRGQVLYSHK
                     DFADELGLSIQNVRTSLSHLKLTGEVTLELTGGLWLATLVQYDFWNPADVKVTGQLTA
                     GDAGNQQANQQGAYIEDSKKRKKVKKESVRTREELRSFLSGEAFTHRSDYVGVHKYLS
                     LYQFIESNGGDHLLQLEKLLTYLEYTELKKFSRDKKVSLPAIIEAMINAKGSFDKSKK
                     TSFYLTARNWIRREQKQGA"
     gene            1152..2624
                     /locus_tag="PK742_05270"
     CDS             1152..2624
                     /locus_tag="PK742_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HQU76033.1"
                     /translation="MKNDKVNQTIIRSSKSLAADMTDIIYGKLPPQAVDLEEAVLGAF
                     MIDRDVLRFLEELKPEYFYDDRHRLICQAIKRLHEKRSVVDILTVTEECRKMGKLEDA
                     GGPFYITSLTSRVASAAHTEHHIRIIQQKYVGRTLILVGTGMVHDAYSDETDIFELLD
                     RSKEMIKEVETGMGLDAGVQTLEDAVVELRRPDKASAPLITGVTGYDRYMDAEPGTLT
                     VVSGRTGMGKTVYIQEEAEACAEAGYPTAIFTMEVSSRVLTARMAADSGVPFERILYN
                     QVKAGEENQLNRRLSHMQSLPLNLIDDPKQLQDLLRLIRRQVFEGIKRIYIDQLRFVK
                     FSEKGFDGVTARSSEVVRQIKMAAKELNIPITLVAQVNREAQKKGGDFRPDLIDLKDT
                     GSLEEDADNVVLLWRPEYYYQKGYDRFERVFCKARGIQREVCSKGLLEVNHAKVRNGE
                     PGIDAWTFDGPRFAIKEYNEQFYKQSHGKKDDDEDDDLPW"
     gene            2662..3813
                     /locus_tag="PK742_05275"
     CDS             2662..3813
                     /locus_tag="PK742_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76034.1"
                     /translation="MSEEYVRSIKPLFNRVVIRIARNFEDNFYQLASGLVLTTDWKPS
                     EYAHVDGEVLAVGQLRYKPLKKENMLSDGSMPLEYDVDKCVIEPGDRVFMLNTAIMDS
                     VAMGLYWSIEGGGFAALVRYDLIVGAVRDGKPFGVNGHILMEKIRIREQQVVDLSVGG
                     DVTQEFMKEISKERFHQKLDSKLIQKIKSDNDGMGKLVGMDQQFYTDAYKVVGLPRDI
                     IRNYGWNPTAKDREDYIHIGQTLLVETCWPLQNTYVRDNSFDFVVCRQEHVAGMLMKG
                     KAEGIVLNSKRILYQAVIRKLTDQDGLIMTTEMGGRYVRGFVVNVGPDTHAMDVSPEN
                     KAHWRGRLIKRGDEVLLSKGARGMQFGKIAMIVHEKDIKFICSAKQIAV"
     gene            3810..4136
                     /locus_tag="PK742_05280"
     CDS             3810..4136
                     /locus_tag="PK742_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76035.1"
                     /translation="MRAGARKLASILWAYNLKWRDPEAWSDCPVEEEFNKLCHIWVRS
                     WQESQGVKEGGPGYFPNCLSWWEELPEVAQRHYADLELTEEERLKEIRFCAPELSGNI
                     KLVNLN"
     gene            4136..4825
                     /locus_tag="PK742_05285"
     CDS             4136..4825
                     /locus_tag="PK742_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76036.1"
                     /translation="MVLPFCESHGLKLTASSFEECWINPEKHKILAKSQQLKDWVEFQ
                     PHPKKFPSHVTNEQVIRYIIAMYDAGSPFLLVKDFNIRKACAGEYAAFKLDEDNMTFS
                     EVVQEILNGEWDVVSQMIVQYCKLSKGVTYSTYVTLYRNYHEKTLADPRIKAKAIKED
                     FEALAEYERKVLAEDQSPEIRRELFRLVAEDELISRSYRPEYKAEEYYLKYGHVALKA
                     EVKNAEEEAGV"
     gene            4800..7094
                     /locus_tag="PK742_05290"
     CDS             4800..7094
                     /locus_tag="PK742_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76037.1"
                     /translation="MPRKKREYKYKSADKFIVYHEEDRDLDTIVITLPKPPPLETIEG
                     YGLPPEQQMFKRTVWPEKFRVLQAKQYENVEDYWDEIERDWEYYKDDMWFIDQEWERR
                     MNGYWLFINGKPTWLCGWHYFYLNYWPMDSDTGDGYSEYRDRDRKSWIFWYWAYTDPL
                     VLGVNYPKHRREGATTRAQCVNFLISTMPMGGFKVEIKSADGGLDAIVKTGVQSKTET
                     DAQDAFRLHFFYAFRKMVFWFKPVSDNNQDPKKMITYKAPAKSPFNQRKKKKAYKLLR
                     SEVDARSSAETAYDSRKLRFYHGDEVGKTIESNVLKRHDVVRECLTLGANTKIIGFAV
                     YTSTVGEMEKKGGKNFASICRQSHFSRRSKTTRQTASGMVNLFIPADEGNDGQTPDGV
                     KFMDEFGASRKQLAHESILALIASKEEAEDWEGAAEVKRLFPLQFRDCFARAGQEERF
                     NMAIIQDVLSHFDVHGYHKNVRFGRFEWYGDPRWEGEAHGYKIHLLPTPDDIMTGKAR
                     VRFVETGPENYDWEISYLFEDDSMSNQWEYDWQRRRIMPKNQRHFSQGSDPFRIRGKT
                     TSGKASNGAGTIYREYDPSVDDPRLERIKNQINPETGDFYWVTDRFCGVYNKRPQMER
                     YCEQQLMASIYYGCRNFSESQVTAIISHYEIRGFHEYLYHYIDDNKKEERENPGHDTS
                     EKIKDEIWNAIADHIEKNGRRELHPTLFEQCEEIDLDMNDYDVFAACGYAKLQSRNRF
                     IHRRNDVPSVQQMNYSQIFPTYDT"
     gene            7091..9316
                     /locus_tag="PK742_05295"
     CDS             7091..9316
                     /locus_tag="PK742_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76038.1"
                     /translation="MRESLLQKYKDEPIGFSDDNVPVAEKNTKEWNLRNAQALWSMHA
                     CGNTVQAITANDAKSGNNKWDYPTLDSYGEGTQDPEKYKQRILGKNPNNEDARKAYVN
                     ISWDNQSVLPRYEDSVLSLIRKSEPNIQPRGVDMTSFSERDRMKFWIKERQSNPFYNR
                     MNERMGGEFVPSLPFQPADQQEIEMAESTTMELNHEISIKEAQELVLNHSEWDEVSFL
                     VRRELFRKGITVCRVEFDHRTRKPRVTNIPADRALFPKSNRRDYDDADTFGHWDYMSI
                     GRIKEMAGLETEDFARFAEDLKNNYGQDVTRAQRADDGTVDSHGGLNNDATVLVLFGE
                     RRSWNTEVRETTKFGSIYHTDLKMEGEKISKSRYEMWYKFCWVVGTDVMLQFGPVEHV
                     YRNRKGRCKSSYIVQKVRNRSIVASAIPIIDEMEIASKKFQIGWAQAAPPGFVVNFRA
                     LQGITWDNQTISPFDVLQIYLQTGRLVVDVDMTDRRKATSFLVQDLPGGLKNLIADAT
                     ATYNMHIEKLNSITGVTEPVLGTAPRPGQLNGVTEMAQEGATNRLENLFDAFGNVKKN
                     IVDRIVRMIQSISMIEGEYEDMFYEMSQLRQHMIKLPARIAHYNYEMVMDQDFSPQMV
                     AKLIGAAEASLSGRNAGDTTQLTYPDYLLVMSLIDKKRFKLAQAIMEYRLKVRAREAQ
                     QINMQNVQANADAQNQSLMTKGKMDENLIRVEGEEDRKGIQEKGRQDRQTQSQQQQNQ
                     E"
     gene            9365..10507
                     /locus_tag="PK742_05300"
     CDS             9365..10507
                     /locus_tag="PK742_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76039.1"
                     /translation="MELEKMEFLGEGGADGPENGNTDTGAGGEGGGEGGNGDGGAGGS
                     GAGDGGAGGGGDDPYVFDDASDKPVVQSLRKALGDQYNPDNVNAILKMVQEVPGLKTQ
                     LQQFESYKPYQPQAQFLAENPGLSEVVKYLQAGGKNVGLFQELASMNLGEMRPEQAIA
                     FQLKFEHGYSDQEVNVYLKQKYGIGEQVDDPLDEGYIMKQAQLKSDGGAAMNWVKEFQ
                     EKAPKSADKRESGPSLEEIGRQWDPVIENAKKTKLDIAHQIVTPTGEKVDVPFQFLSN
                     DEIEARTVRIARGLALDLGLPLTEQNQNLILTLARNAAIGMAVPNIVKSAMTAAAKSG
                     MDYMSKQYAGNPGLGGGNLNGGGITDGELKFVSETGQNQDKNKDYW"
     gene            10633..>10907
                     /locus_tag="PK742_05305"
     CDS             10633..>10907
                     /locus_tag="PK742_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76040.1"
                     /translation="MERRGDQWAGSATKIVQSLGFERPASNETVNHWEDDSYMRPVKI
                     GATVSAGQGGSGAAFDITLAAGEVFTDDNGGKHIFPRVGDVLMFPNVT"
BASE COUNT         2959 a         2583 c         3196 g         2169 t
ORIGIN      
        1 ttaccgtttc catcagtgga gccggagtca gaaatatata gataagccca cccctttgtg
       61 tgtaacgtct gttgatcagg gaggtaaatt tcccaggaga agggggtgtc tggggcgcat
      121 ttccccgtaa catggagaaa gtgacgagga aataaaagtt ttgcacctac taaatcggcg
      181 ataatgatgc gttattacgg gaaaacaccc tttttcggtg tgacatgaaa atagtgtcct
      241 atttggagaa atgggtggga tgtagcgaat attgcgctgt gaccaatatc gtgaaaagcc
      301 atcgaactta tttaaagaaa cctctccggg ccgcgggtga tattggtcac gctcttcttc
      361 cgtgtgcctt ggaggggctt tttatggctt atggcgaacg acaggtataa aggcagttgg
      421 atccgtgcgt atcgttccct gatggattgg gagtggtatc gggacgggaa taccatgcgg
      481 gtgtggatgc atctgctgtt aaaggcggag taccaggacc gggagtatag gggtgtagtg
      541 atcaggcgag gccaggtctt gtatagccat aaggattttg ctgatgaatt gggcctgagt
      601 attcaaaacg tacgtaccag cctatcgcat ctgaaactaa caggcgaagt aacattggaa
      661 ctaacagggg gtttatggct tgcaaccctt gtgcagtatg acttctggaa ccctgctgat
      721 gttaaggtaa cagggcaact aacagctgga gacgcaggca atcaacaggc aaatcaacag
      781 ggcgcatata tagaggattc taagaaaaga aagaaagtaa agaaagagag tgtgcgtacg
      841 cgcgaggagt tgaggtcatt tctgtcaggg gaggccttta ctcacagaag cgattatgta
      901 ggcgttcaca agtacttgag cctttaccag ttcatcgaga gcaacggcgg agatcatctg
      961 ctgcagctgg agaaactact gacgtacctg gagtacacgg agctgaagaa gttcagccgg
     1021 gacaagaagg ttagtctccc ggcgatcatt gaggcgatga tcaatgccaa aggtagtttt
     1081 gacaagagca agaagaccag tttctacctg acggctcgga actggatacg cagggagcaa
     1141 aaacaaggag catgaagaac gataaagtaa atcagaccat catccgcagc agcaagagct
     1201 tggctgcgga tatgacggat atcatttacg ggaagctgcc tccgcaggct gtggatttgg
     1261 aagaggcggt tctgggtgcg ttcatgatcg accgggacgt gctgaggttt ttggaggagt
     1321 tgaagccgga gtatttctac gacgaccggc accggctgat ctgccaggcg atcaagagac
     1381 tgcatgagaa gcgatcggtg gttgatatcc tcaccgtcac cgaggagtgc cggaagatgg
     1441 gtaagctgga agatgcaggc gggccgtttt acatcacgag tctgaccagt agggtggcgt
     1501 cggcggcgca caccgagcac cacatcagga tcatccagca gaagtacgta gggcggacgc
     1561 tgatcctggt aggtacagga atggtacacg acgcatacag cgatgaaacc gacatcttcg
     1621 aattgctgga ccgcagcaag gaaatgatca aggaggtgga gaccggcatg gggctggatg
     1681 cgggcgtgca aaccctggag gatgctgtgg tggaactccg gcggcctgat aaagcctcag
     1741 cgccgctgat cacgggcgtc accggatatg accggtacat ggatgccgag cccggtacgc
     1801 tgacagtggt gagcggccgg acgggtatgg gtaagaccgt gtacatccag gaagaggcgg
     1861 aagcctgcgc tgaggcggga tatcccacag ccattttcac tatggaggtc agctccagag
     1921 tgctgacggc aaggatggca gcggattcag gcgttccctt cgagcggatc ctctacaacc
     1981 aggtgaaagc cggggaggaa aaccagctga accgaaggct gtcgcatatg caatcgcttc
     2041 cgctgaatct gatcgatgac ccgaaacagc tgcaggatct gttgaggctg atccgccgtc
     2101 aggtgttcga aggcatcaag cgcatctaca tcgaccagct gaggttcgtg aagttctcgg
     2161 agaaaggctt tgacggagtc actgcccgat catcagaggt ggtgcggcag atcaaaatgg
     2221 cggcgaagga gctgaatatc cccatcacgc tggtggctca ggtgaatcgg gaggcgcaga
     2281 agaaaggcgg ggatttccgc cccgacctga tcgacctcaa ggataccggc agtctggaag
     2341 aagatgctga taacgtcgtg ctgttgtgga gaccggaata ttactaccag aaagggtatg
     2401 accggtttga gcgggtgttc tgcaaagccc gcggcatcca gcgtgaggtg tgcagcaagg
     2461 gcttactgga agtcaatcac gccaaggtca ggaacggcga acccggcata gacgcctgga
     2521 cgttcgacgg gccgaggttt gcaatcaaag agtataatga acaattttac aagcaatcac
     2581 atggaaaaaa agacgacgac gaggacgacg acctcccctg gtgatatcaa ttcgccggag
     2641 gaagtgctga aacggtttac cgtgtctgag gaatatgtcc gcagcatcaa acccctgttc
     2701 aaccgtgtgg tgatcaggat cgcccgcaat ttcgaagaca acttctacca gctggcatcc
     2761 gggctggtgc tgaccacgga ctggaagccc agtgaatacg cccatgtgga tggcgaggta
     2821 ctggctgttg gacagcttcg gtacaaaccc ctgaagaaag aaaacatgct gtcagacggc
     2881 agtatgcccc tggaatatga tgtggacaaa tgcgtgatcg agcccggaga cagggtgttc
     2941 atgctcaaca ccgcgatcat ggattctgtg gccatggggc tgtactggag catcgagggc
     3001 ggtggtttcg ccgctctggt caggtacgac ctgattgttg gcgctgtcag agacgggaag
     3061 cccttcggtg tcaatggcca catcctcatg gagaagatcc gcatccggga acagcaggtc
     3121 gtggacctga gtgttggcgg tgacgtcacc caggagttca tgaaggagat ctccaaagag
     3181 aggttccatc agaagcttga tagcaagttg atccagaaga tcaagtccga caacgatggt
     3241 atgggtaagc tggtgggcat ggatcagcag ttctacaccg acgcctacaa ggtcgttggc
     3301 ctgcccaggg atatcatcag gaactacgga tggaacccaa cggcaaagga cagggaggac
     3361 tacatccata tcggacagac gctcctggtg gagacgtgct ggccgctgca gaacacctac
     3421 gtcagggata attcctttga tttcgtggtc tgccggcagg agcatgtggc ggggatgctg
     3481 atgaagggca aggcagaggg catcgtcctg aactcaaagc ggatcctcta ccaggctgtt
     3541 atccggaagc tcaccgatca ggatgggttg atcatgacca cggaaatggg aggccggtac
     3601 gtcagaggct ttgtcgtcaa tgtcggaccc gacacccacg ccatggatgt gagccctgag
     3661 aataaggctc actggagggg aagactgatc aagcggggcg acgaggtgct gctgagcaaa
     3721 ggcgctcgcg gcatgcaatt cgggaagatc gctatgatcg ttcacgaaaa agacattaaa
     3781 ttcatttgca gcgcaaaaca aattgcggta tgagagctgg agcaagaaaa ttagcatcga
     3841 tcctttgggc atataacctg aagtggcgtg accccgaagc ctggtctgac tgtccggtgg
     3901 aagaggaatt caacaaactc tgccatatct gggtgagaag ttggcaggaa tcgcagggcg
     3961 tgaaagaagg aggcccggga tatttcccca actgcctgag ttggtgggag gaactgccgg
     4021 aggtagcgca gcggcattac gccgacctgg aactgacgga agaagaacgc ctgaaggaga
     4081 tccggttctg cgcacccgaa ctcagcggta acatcaaact cgtaaacctg aactgatggt
     4141 actaccgttc tgcgagagtc acgggctgaa actgacggct tcctccttcg aggaatgctg
     4201 gatcaatccg gagaagcata agatcctggc gaaatcccag cagctgaagg attgggtgga
     4261 gttccaaccg cacccgaaga agtttccctc ccatgtcacc aacgaacagg tgatcaggta
     4321 catcatcgcc atgtatgacg ccggctctcc tttccttctg gtcaaggact tcaatatccg
     4381 caaagcctgt gccggcgaat acgctgcgtt caagctggac gaggataaca tgaccttttc
     4441 cgaggtcgtg caggagatcc tgaacggtga atgggatgtg gtgtcgcaga tgatcgtgca
     4501 gtactgcaag ctcagtaagg gcgtgaccta ttccacctat gtcacgctat accggaacta
     4561 tcacgagaaa accctggctg atccacgaat taaggcgaaa gccatcaaag aagactttga
     4621 ggctctggcg gaatacgagc ggaaggtgct ggcagaagac cagtcgccgg agatccgcag
     4681 agagctgttc cggctcgtgg cagaggatga actgatctcc agaagctacc ggccagagta
     4741 caaagccgag gaatactatc tgaaatatgg gcacgttgcg ttgaaagcag aagtgaagaa
     4801 tgccgaggaa gaagcgggag tataaataca agtcagcgga caagttcatt gtctatcacg
     4861 aggaagatcg cgacctcgat accatcgtta tcaccctacc taagccccct ccgctggaaa
     4921 ccattgaggg gtatggcctg ccgcctgagc agcaaatgtt caagcgcacg gtgtggccgg
     4981 agaagtttcg cgtcctgcaa gcgaagcagt acgagaatgt tgaggactac tgggacgaga
     5041 tcgagcggga ctgggagtac tacaaagacg acatgtggtt catcgaccag gaatgggagc
     5101 ggagaatgaa cggctactgg ctgttcatca acggcaaacc tacctggctg tgcgggtggc
     5161 attacttcta tctgaactac tggcccatgg actccgacac cggagacggg tacagcgaat
     5221 accgtgaccg cgatcggaag agctggatct tctggtattg ggcgtacaca gatcctctgg
     5281 tgctgggtgt gaactacccc aaacaccggc gggaaggcgc taccacaagg gcgcagtgcg
     5341 tgaatttctt gatatcaacc atgccaatgg gcgggttcaa ggtggagatc aagtccgcag
     5401 acggcggcct cgatgccatt gtcaagaccg gcgtgcagtc gaaaaccgag acggatgccc
     5461 aggatgcgtt caggctgcat ttcttctatg ccttccggaa aatggtgttc tggttcaagc
     5521 cggtctctga caacaaccag gatccgaaga agatgatcac ctacaaggcg ccggcgaaat
     5581 ccccgttcaa ccagaggaag aagaaaaagg cctacaagct gctgcgcagt gaggtggatg
     5641 ccagatcatc agctgaaact gcctacgata gccggaaact caggttctac cacggggatg
     5701 aggttggtaa aaccatcgaa tccaacgtcc tgaagcggca tgatgtcgtc agggagtgtc
     5761 ttaccctggg agccaatacg aagatcatcg gcttcgccgt ctatacctcc acagtggggg
     5821 aaatggaaaa gaagggcggt aagaactttg cttcgatctg ccggcagagc catttcagcc
     5881 gccgcagtaa gaccaccagg cagacagcat cgggcatggt gaacctgttt atcccagccg
     5941 acgaaggcaa tgacggccag acacctgatg gcgtgaagtt catggatgaa ttcggagcct
     6001 ccaggaagca gcttgcccat gaaagcatcc ttgccctgat cgcttccaaa gaagaggcgg
     6061 aggattggga aggtgctgca gaggtaaaaa ggctgttccc gctgcaattc cgtgactgtt
     6121 tcgcccgggc ggggcaggag gaacggttca atatggccat catccaggat gtcctcagcc
     6181 atttcgatgt gcacggctat cacaagaacg tccggttcgg ccggttcgaa tggtacggag
     6241 accccaggtg ggaaggagag gcgcacggat acaagatcca cctgctgcca actcctgatg
     6301 atatcatgac cggcaaagcc cgggtaaggt tcgtggagac gggcccagag aattacgatt
     6361 gggagatctc ctacctgttc gaggacgatt ccatgtccaa ccaatgggag tatgactggc
     6421 agcggcgccg gatcatgcca aagaaccagc ggcacttcag ccagggcagt gacccctttc
     6481 gcatcagggg caagaccaca tccgggaaag ccagtaatgg cgccggcact atttaccgtg
     6541 agtatgatcc cagcgtagat gatccccgcc tggaaaggat aaagaaccag atcaacccgg
     6601 aaaccggtga tttctactgg gtgaccgaca ggttctgcgg ggtctataac aagcgcccac
     6661 agatggagcg ttactgtgaa cagcagttaa tggctagtat ttactacggt tgccgtaact
     6721 tcagcgaatc gcaggtgacg gctataatca gccattatga aattcgtggc tttcatgagt
     6781 acctttatca ctacattgac gacaacaaaa aagaggaaag agagaaccct ggccatgata
     6841 ccagcgagaa aatcaaggac gagatctgga atgccattgc tgatcacatc gagaagaacg
     6901 gccggcgcga actccacccc acactttttg agcagtgcga agaaattgac ttggatatga
     6961 atgactatga tgtatttgct gcctgcggat atgcaaagtt gcaatcacgg aaccggttta
     7021 tacaccggag aaatgatgtt cccagtgtcc agcaaatgaa ttattcccaa atctttccaa
     7081 cctacgacac atgagagagt cgctgcttca aaagtacaag gacgagccga taggcttttc
     7141 cgacgacaat gttccggtag cggaaaaaaa tacaaaggag tggaacctcc gaaacgcaca
     7201 agccctgtgg agcatgcatg cttgcgggaa cactgttcag gccatcaccg ccaacgatgc
     7261 gaagtctggg aataacaaat gggattaccc caccttggac tcctacggag agggcacgca
     7321 ggatcccgaa aaatacaaac agcgcattct cggcaagaac cccaataacg aagatgcccg
     7381 caaagcctat gtgaatatca gctgggataa ccagtcggtt ctccccaggt atgaggacag
     7441 cgtgttgtcg ctgatccgca agtccgaacc caacatacag ccgcggggtg tggacatgac
     7501 cagcttcagc gagagagatc gcatgaagtt ctggatcaaa gagcgtcaga gcaatccctt
     7561 ctacaacaga atgaatgagc ggatgggcgg agaattcgtg ccatcgctgc cgttccagcc
     7621 agcagatcag caggaaattg aaatggcgga gtccactacc atggaactca accatgagat
     7681 cagcatcaaa gaggcacagg agttggtgct gaatcacagc gaatgggatg aggtgagttt
     7741 cctggtacgc cgggagttgt tccggaaagg catcaccgtc tgcagggtgg agttcgacca
     7801 caggacccgc aagcccagag taacgaatat ccccgccgac cgtgctcttt tcccgaaaag
     7861 caatcgccgc gactatgatg atgcagatac cttcgggcac tgggattata tgagcatcgg
     7921 caggatcaag gaaatggccg gtctggaaac agaagacttt gcgaggtttg ccgaggatct
     7981 gaaaaataac tacggccagg acgtcacaag ggcgcagagg gctgacgacg gcacagttga
     8041 tagccacgga ggcctcaata acgacgctac ggtattggtg ctctttggag agcgccgctc
     8101 ctggaacacc gaggtcaggg aaaccacgaa gttcggcagc atctaccaca ccgatctgaa
     8161 aatggaggga gagaaaatct ccaagagccg gtatgaaatg tggtataagt tctgctgggt
     8221 ggtaggaact gatgtcatgc tgcaattcgg tccggtggag catgtctatc gcaaccgcaa
     8281 gggccggtgc aaatcctctt atattgtcca gaaggtgaga aatcgtagta tagttgcttc
     8341 cgccatcccc atcattgacg aaatggagat cgccagcaag aagtttcaga tcggctgggc
     8401 tcaggcagcg cctccgggct ttgtcgtgaa tttccgggct ttgcagggta tcacctggga
     8461 caatcaaaca atttctccct ttgatgtcct gcagatctat ctgcaaaccg gccgcctggt
     8521 cgtggatgtg gatatgaccg accgcagaaa agctacttcc ttcctggtgc aggatctccc
     8581 tggcggtctg aagaacttga tcgcagacgc cacagccacc tacaacatgc acatcgagaa
     8641 gctgaactcc ataacagggg taacggagcc ggtgctggga accgcaccga ggccggggca
     8701 gctgaatggc gtgacggaaa tggcgcagga aggagcgacc aaccgactgg agaacctgtt
     8761 tgacgccttc ggcaatgtca agaaaaatat tgtagatcgc attgtccgga tgatccagtc
     8821 catttccatg atagaagggg agtacgagga tatgttctac gagatgtctc agctcagaca
     8881 gcacatgatc aaactgccgg cacggatagc tcattacaac tatgaaatgg tcatggatca
     8941 agacttcagt ccgcagatgg tggcaaaact cataggcgcc gctgaagcaa gtctgtctgg
     9001 aaggaatgcc ggcgacacca cacagttgac gtatccggat tatcttctgg tgatgtcctt
     9061 gatcgataaa aagcggttca agctggctca ggctattatg gaataccgtc tgaaagtcag
     9121 ggctcgcgag gcgcagcaga tcaatatgca gaatgttcag gctaatgcgg atgcgcagaa
     9181 tcagagcctt atgaccaaag gcaaaatgga tgaaaacctg atcagggttg aaggtgaaga
     9241 agacagaaag ggtatccagg aaaaaggacg ccaagaccgt cagactcaat ctcaacagca
     9301 gcaaaatcaa gaataatttg aaaatttcgt tacagcactt aaatttgtag gacaaaggcg
     9361 aactatggaa ctggaaaaaa tggagttttt aggcgagggc ggagcagatg gccccgaaaa
     9421 cggtaatacc gacacaggcg caggtggcga aggtggaggt gaaggcggca acggagacgg
     9481 cggagcaggt ggctcaggag ccggagacgg tggcgcagga gggggaggcg acgatcccta
     9541 tgttttcgac gacgcatctg ataagcccgt agtccagtca ctccgtaaag cgctgggaga
     9601 ccagtacaat ccagacaatg taaatgcgat tttgaagatg gtacaggaag tacccggtct
     9661 gaaaacgcag ttgcaacagt tcgaatctta caaaccgtat caaccgcaag cacagtttct
     9721 ggcggaaaat ccggggctat ccgaagttgt caaatacctc caggcgggag gtaaaaacgt
     9781 agggttgttc caggagttgg cctccatgaa cctcggtgaa atgcgcccgg aacaggcgat
     9841 agctttccag ctgaagtttg agcacggtta cagcgatcag gaagtcaatg tgtatctgaa
     9901 acagaagtac ggaattggcg aacaggtcga tgatccactg gatgaaggtt atatcatgaa
     9961 gcaagctcaa ctcaagagtg acggcggggc cgccatgaac tgggtgaagg agtttcagga
    10021 aaaagcacct aaatcggctg acaaacgtga gtcaggccca agtctcgaag agataggccg
    10081 tcaatgggac ccggttatcg agaacgctaa aaagaccaaa ctggacatcg cccatcaaat
    10141 cgtcactcct actggagaaa aagtagatgt tcccttccaa ttcctgagca acgacgagat
    10201 cgaagccagg accgtgcgaa tcgccagagg gctcgcactt gatctcgggc tcccgttgac
    10261 ggagcagaat cagaacctta ttcttactct tgccagaaac gcagcaatcg ggatggcagt
    10321 gccaaacatc gtgaaatcag cgatgaccgc agctgcaaaa tcaggcatgg attatatgtc
    10381 taaacagtac gctggaaacc ccgggctcgg aggcggtaac ctcaatgggg gtggtattac
    10441 agatggagaa ttgaagtttg tgagtgaaac gggccaaaac caggacaaaa acaaagacta
    10501 ctggtaaggc ctttgatctg ttaatacaga atccaaatgg cacaagataa taaagcccct
    10561 gtccagggta ttcacgccga cgcatatttg tcggcattgg acatcaatga gccggataat
    10621 ctccgcgtgc tcatggagcg cagaggagat cagtgggctg gctctgcgac caaaatagtc
    10681 cagtctctcg gctttgaaag gccggcatct aacgagactg tgaaccactg ggaagatgat
    10741 tcctacatgc gtcctgtcaa gatcggtgct accgtttccg caggtcaggg tggatccgga
    10801 gctgctttcg acatcacgct ggcagccggt gaagtcttta ccgatgataa cggcggtaag
    10861 cacattttcc cgcgtgtcgg ggacgtgctg atgttcccga acgtgac
//
LOCUS       DAOMAX010000067        10481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_855_length_10481_cov_4.004988, whole genome shotgun sequence.
ACCESSION   DAOMAX010000067 DAOMAX010000000
VERSION     DAOMAX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_855_length_10481_cov_4.004988"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..744
                     /locus_tag="PK742_05310"
     CDS             109..744
                     /locus_tag="PK742_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76041.1"
                     /translation="MDYNCFGILAFAEVRPVLSIMQVKMYLAVSVAVMLLVSCAKAER
                     ETDLYVRMYKVQYRPTTNDKEILTDQKVHKQIRIDRTETGIILTDEIYDVDSKTFHSP
                     FVSWSISFNRNDIVAVGKYAGDSINDLKLEYAIIDSIPVMINATEEQLYILRSVDPPV
                     DANGLLYLSLKYGVIFQYSLDWLIYLYLYEKQSLQYDVDELLRSAQRYYQD"
     gene            complement(810..2300)
                     /locus_tag="PK742_05315"
     CDS             complement(810..2300)
                     /locus_tag="PK742_05315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQU76042.1"
                     /translation="MDFGRYTQHLLAAAFILAAIAATGIFRYMEGPPRGVHQWRQTDC
                     ASIALNYFQDSPGFFHPQEHNLTGENGATVSEFPVLYYLDAQLYRLFGPHELIMRLVN
                     LLFLVAGLGYLYRLGMLLHGSFLLAMIPVLLLISSPVWVYYGVHFLPNVPAISCCIAG
                     WFYFFQWRETPSKKWLITWLVLFLSGALLKTSDGMSLLTAGIILLLQERKEAWWKRGW
                     FAGGLVTLMLLGIWIMFAAWYNRIHQNELSLIGILPFWQYDEDYFQSIAARFYRDWSL
                     HLFHPAVWVILGLMCLQVFVFFKRLPVLLRQVISWTGIGSVVYLLLWTEPLYHHDYYM
                     LTPFIFLIFLVIGSMYLLRRRTAVVSNAIGGILTAGSLAVILLYQPGMQELRNQGVQY
                     ENVASGFYTIGPWLESMGVPPDARVISVGDQTRNISLYLMNRRGWTDVYNRRKITYQQ
                     KVDEGARYVVVSGEPTKQSEDTPVDSLTFLGEYEGIRLYEIPGSVN"
     gene            2396..4279
                     /locus_tag="PK742_05320"
     CDS             2396..4279
                     /locus_tag="PK742_05320"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQU76043.1"
                     /translation="MASNDYNYSEEHIRTLDWIEHMRLRPGMYIGKLGDGTSPDDGIY
                     ILVKEVVDNAIDEFAMGFGKKIEIRVDKQQVMVRDYGRGIPLGKVIDCVSKMNTGAKY
                     DSKAFQKSVGLNGVGTKAVNALSSWFRVQSTREGKSKVAEFEKGVITKDNKIAASTDR
                     DGTLVEFIPDPDIFRNFHFIPEYLENQIWNYAYLNAGLTIQFNGQKYYSQNGLLDLLE
                     RKTNKEELRYPIIHLKGEDIEIAMSHNNDYGEEYYSFVNGQFTTQGGTHLAAFREAVV
                     KTIREFYKKDYDASDIRQSIIAAVSVRVQEPVFESQTKTKLGSLNMAPDGPAVRTFIN
                     DFVRRELDNYLHKNPQAAEALQKRIMQSERERKEISGIKKLANERAKKANLHNRKLRD
                     CRFHFSEDKDPRANDTMLFITEGDSASGSITKVRNVETQAVFSLKGKPLNVFGLTKRV
                     VYENEEFNLLQHALNIEDGVDGLRYNQIVIATDADVDGMHIRLLLLTFFLQFFPEVVK
                     NGHLYILQTPLFRVRNKKETIYCYDEEEKQKAMAKLGGKPEITRFKGLGEISPEEFAG
                     FIGDDIRLEPVMIGTEKKVQELLQYYMGKNSMQRQEFIIKNLRIEKDTIIENATVEET
                     TES"
     gene            4276..4755
                     /locus_tag="PK742_05325"
     CDS             4276..4755
                     /locus_tag="PK742_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76044.1"
                     /translation="MKSYLIILSAGMLTFAASCASVEQENDTSALESRIDSLEAEIAE
                     LKTELAPGLAVLMNQNYDHLKKLEVAINEGNWEYADFCLHEMDETFEQIVDLHNNHDE
                     LVQPADTQFKAFIFPTFEQLEEKVDTKDQAGASQLFIELKTNCSKCHTANNHGFIRL"
     gene            4771..7362
                     /locus_tag="PK742_05330"
     CDS             4771..7362
                     /locus_tag="PK742_05330"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HQU76045.1"
                     /translation="MEETNKREEGQVYHVSGMYKDWFLDYASYVILERAVPDSRDGLK
                     PVQRRILHAMKEMDDGRFHKVANIIGQTMQYHPHGDAAIGDALVNMGQKDLLIDTQGN
                     WGDVRTGDSAAAPRYIEARLSKFALEVVFNKDTTEWQLSYDGRKPEPVSLPVKFPLVL
                     AMGVEGIAVGLSTKILPHNFCELLEASIACLKGEEFQLFPDFATGGFIDVSNYDDGMR
                     GGKVRVRAKLEVADKKTIIIREIPYGTTTTSIIDSILKANDKGKIKIKKVTDNTAKDV
                     EILIELPAGVSPDVTVDALYAFSDCEVSISPICCVIRKDKPVFVPVSELLKDSAMFTK
                     GLLKQELEIRLAELEDKWHYSSLEKIFIENRIYRDIEECETWEAVIETIDRGLDPFKK
                     NLQREVTRDDIVRLTEIKIKRISKYDSFKADELIRSIEAEIKEVKNNLKNLTKYTIAW
                     FQGLLDKFGKGRERKTEIKVFDVIEAKAVVANNTKLYMNRADGFVGFGLKKEEFVCEC
                     SDLDDIIVFTEEGIMKVVRIADKVFIGKKIIHAGVWRKDDDRMTYHAIYTDGKTGKSF
                     AKRFNVTSITRDKEYNITQGTPRTKVWYFTANPNGESETVNIQLHPNARAKIKNFDFD
                     FGQLAIKGRSAMGNTVTKYPLKKLVRSEVGSSTLGGRGLWLDTTVGRLNRDNRGQYLG
                     EFDTGDMILVLFKDGNYELTDFELTNRYEMREIEQVSKFDADEVITAIYYEGESGVTY
                     VKRFYVEATVPGKKYPFISETKGTQLIYVSTEEQPRISMTILKGKKEEEVTEEIDLAS
                     FIDIKGWKSQGNRLSPHTIKEIAGLVAEEEPETEDDNVGIGGTIEWDLEKGDKKVKGG
                     KGQGELF"
     gene            complement(7354..8271)
                     /locus_tag="PK742_05335"
     CDS             complement(7354..8271)
                     /locus_tag="PK742_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQU76046.1"
                     /translation="MAMRGKWMFKALVQKTLSVLPGSHHLNYLFQRHVTKGVQLSDQY
                     FSDKLQHARDHIRLYRAHTGRNCPSSTIELGTGWYPIVPFYFYLCGAENVMTTDISRH
                     CKPGFALETARRILDEIPDEPGFQDDRKAQLTAWLQAPKSDTRTMLSPLGIDYRVGDA
                     RQSGLPGDSVEFIHSNNTFEHVYPDILRDILKEFRRIGKPDGLHSHFIDMSDHFAHMD
                     SSISIYHFLRYSEAQWKRIDNSIQPQNRWRLSQYHQLFRETGYQLITEEIRPGHKAQL
                     EKEPLHSSYTSFDTADLAVSHAHVLLRSE"
     gene            8568..10181
                     /gene="pckA"
                     /locus_tag="PK742_05340"
     CDS             8568..10181
                     /gene="pckA"
                     /locus_tag="PK742_05340"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQU76047.1"
                     /translation="MKAVGHKNPNATLEQYGLKHSATSYWNLTPAELTEHSISRGMAE
                     MADSGALVVDTGKFKGRAPKDKYTVKDAKTENTVDWNNFNIPTDNATFDHLYNKVMAY
                     LADKDVWVRDAYACADPKYRLNIRVINEFPWGNMFCYNMFLRPTAEEIDTMTNDWLIV
                     NAPGFQADPATDNVRSANFAMIDFTKQIILIGGTGYTGEMKKGIFTVLNYVLPQDKGV
                     LAMHCSANVGKDGDTAVFFGLSGTGKTTLSADPDRKLIGDDEHGWTEDTVFNFEGGCY
                     AKCVDLTKEKEPQIFDAIKFGSILENIEYVPGTRTVDFTNVKKTENTRVSYPIDYIDN
                     IMVPSMGGTPKNIFFLTCDASGVLPPISKLTPGQAMYQFLSGYTAKVAGTEAGVTEPQ
                     LTFSTCFGAPFLPLHPTKYAEMLGEKMRKHNVNVWLINTGWTGGPYGVGNRIKLSYTR
                     AMITAALNGELDKVSYKKHEVFGLMMPETCPNVPDEKLDPRSTWADQKAYDEKAANLA
                     AQFVKNFEKYADKANEEILAAAPKVPANA"
BASE COUNT         2926 a         2591 c         2622 g         2342 t
ORIGIN      
        1 catcaccctc gagaaaggcg aacgcagcga actggccaac ctggcgaacg aattgctgtg
       61 atctgaaaat attttacagt ccccacagtg tttcccaaag ggaacgctat ggattataat
      121 tgctttggca ttttggcgtt tgctgaagtc cggcctgtat tatccatcat gcaagtaaaa
      181 atgtatctag ctgtttccgt ggctgtaatg cttcttgtgt cctgcgctaa agcagaacga
      241 gagactgatc tttatgtacg aatgtataag gtgcaataca gaccgaccac caatgacaaa
      301 gagatactta ctgatcaaaa agtgcataag cagatcagga tcgacaggac tgaaactgga
      361 attattctga cggatgagat ctatgatgtt gactcaaaga cctttcattc tccttttgtt
      421 agctggagta tatcatttaa tagaaatgat attgttgctg taggaaaata tgctggtgat
      481 agcatcaatg atttgaagtt ggaatacgcg atcattgatt ccataccggt catgatcaat
      541 gctactgagg agcaacttta tattctgcga tctgtagatc ctcctgttga tgcaaatggg
      601 ctgttgtatc tctccttgaa gtatggtgta atatttcaat acagcctgga ttggctgata
      661 tatctgtatt tgtatgaaaa acaaagttta cagtacgatg ttgatgaact attacgatcg
      721 gctcagagat actaccaaga ttaatattat ttgaatgttc ccacagtgtt gcacgcccgc
      781 ccgccgaagg agggcaggga actctgtggt cagttgacag atccgggtat ctcatataac
      841 ctgataccct catactcacc cagaaatgtc agcgaatcta ccggcgtatc ctcgctttgt
      901 ttggtgggct ctccacttac caccacatac ctcgcacctt cgtccacttt ttgctgatag
      961 gtgattttcc gtctgttgta cacatccgtc cagcctcgcc tgttcatcag atacagcgat
     1021 atatttctgg tctggtctcc tacactgatg accctggcat cgggaggtac tcccatgctc
     1081 tccagccagg ggccgatcgt gtagaagccc gatgcaacgt tttcatattg tacaccctgg
     1141 ttccgcagct cctgcatgcc cggctgatac aaaaggatga ctgcaagcga acctgcggta
     1201 agtattccgc caatagcatt actgacaact gcagttcgtc tgcgtaacag atacatgctg
     1261 ccaatgacaa gaaagatcag aaatataaaa ggtgtcagca tgtaataatc atggtggtac
     1321 aagggctcgg tccacagcaa cagatacacg acagatccga tacctgtcca gctgatcacc
     1381 tgcctcagca ataccggtaa ccttttgaaa aacacaaaaa cctgtaggca catcagcccc
     1441 agaataaccc atactgccgg atgaaataga tgcaggctcc agtcccggta aaatctggcc
     1501 gcaatacttt ggaaatagtc ttcgtcatat tgccagaaag gcagtatgcc tatcagactg
     1561 agttcattct ggtggatccg gttataccat gcagcgaaca tgatccatat tcccagcaac
     1621 ataagtgtta ccagtccgcc ggcaaaccac ccccgcttcc accaggcctc ttttctttcc
     1681 tgcaggagaa gaatgatacc ggcagtcagc agactcatgc catctgaggt cttcagcaag
     1741 gcacctgaca ggaagagcac cagccaggtg atcagccatt tttttgatgg cgtttctctc
     1801 cactgaaaaa agtagaacca gcctgcaata cagcagctga tggccggtac attcggcaga
     1861 aagtgtacac catagtacac ccagacaggg gagctgatca ataacagaac gggtatcatc
     1921 gccagaagaa aactgccgtg tagcaacatc ccaagccggt acaggtaccc aagccctgcc
     1981 accaggaaca aaagattcac gaggcgcatg atcagttcat gcggaccaaa cagacgatat
     2041 aactgggcat ccagatagta cagaaccgga aattcgctga cggtggctcc gttttctccg
     2101 gtcagattgt gctcctgtgg atgaaaaaat cccggagaat cctggaagta attcagagca
     2161 atggaggcac agtctgtctg tcgccactgg tgcacacctc tgggaggccc ttccatgtac
     2221 cggaatatgc ctgtggccgc gatggctgca agaatgaatg ctgcagccaa caaatgctgt
     2281 gtatatctcc cgaaatccat gaacgtccaa taatacacat tccccctgca tgttgtgcat
     2341 attttgaagg gagaaaaatg ggctgaagcc tgcatcaggg ctaattttgc cacctatggc
     2401 ctcgaacgac tacaactatt ccgaagaaca tatccgaacg ctcgactgga tcgagcatat
     2461 gcgcctgcgt ccgggtatgt acatcggaaa gctgggcgat ggtacctctc cggatgacgg
     2521 tatctacatc ctggtaaagg aagtggttga taatgccatt gatgagttcg ctatgggttt
     2581 cggcaagaag atcgagatca gagtggacaa gcagcaggtc atggtgcgtg actatggcag
     2641 gggcatcccg ctggggaagg tcatcgactg tgtcagtaaa atgaacacag gtgccaaata
     2701 cgacagcaag gcttttcaga aatctgtcgg tctgaatggt gtgggtacca aagcagtcaa
     2761 tgcactgagt tcctggttcc gggtacagag taccagagaa ggtaagtcca aagtggccga
     2821 gttcgagaag ggtgtcatca ccaaagacaa taagatcgct gccagtactg accgtgatgg
     2881 tacactggtg gaattcattc cggatcctga catcttccgc aatttccatt tcattccgga
     2941 atacctggag aatcagatct ggaattatgc ctacctgaat gcaggactta ctatccagtt
     3001 caacggtcag aaatactatt cacagaatgg tctgctcgat ctcctggaac gcaagaccaa
     3061 caaggaagaa ctgcgctatc ccatcatcca cctgaaaggg gaggatatcg agatcgccat
     3121 gtcgcacaac aacgactacg gcgaagaata ctacagcttc gtgaatggtc agttcactac
     3181 ccagggcggc acgcacctcg ctgcattccg cgaagcagtg gtaaagacca tccgggaatt
     3241 ttacaagaaa gattatgacg catccgacat ccgccagagc attatcgcgg cagtgtcggt
     3301 acgggtgcag gaacctgtgt tcgaaagcca gaccaagacc aaactcggat cgctgaacat
     3361 ggcaccggac ggtccggccg tgcgcacctt catcaatgat tttgtgcgac gagaactgga
     3421 taactacctg cacaagaatc cgcaggctgc tgaagcgctg cagaagcgca tcatgcaaag
     3481 cgagcgggag cggaaagaga tatccggcat caagaaactg gcgaatgaac gggccaagaa
     3541 agccaacctg cacaacagga aactgcgtga ctgccgcttt cacttcagtg aagacaaaga
     3601 cccccgggcc aatgatacca tgctcttcat cactgaaggt gattctgcct caggttccat
     3661 caccaaggtg cgcaatgtgg agacacaggc ggtcttttct ctgaaaggta aaccgctcaa
     3721 tgtgttcggt ctgaccaaaa gggtggtgta tgagaatgaa gagttcaacc tgctgcagca
     3781 tgcactgaat attgaagatg gtgtggatgg attgcgttac aaccagatcg tcatcgctac
     3841 cgatgccgat gtggatggca tgcacattcg tttgctgctg ctcactttct tcctgcagtt
     3901 ctttccggaa gtggtcaaga acggtcacct gtatattctc cagacacctc tgttccgtgt
     3961 gcgcaacaag aaagagacca tttactgcta cgacgaagaa gaaaaacaga aggccatggc
     4021 caaactgggg ggcaaaccgg agatcacccg gttcaaaggt ttgggagaga tctcaccgga
     4081 agaatttgcc ggattcatcg gagatgacat acgtctggaa ccggtcatga taggtacgga
     4141 aaagaaagtg caggaactgc tccagtacta tatgggaaaa aactccatgc aacgccagga
     4201 gttcatcatt aaaaatcttc gcatcgagaa agatacgatc atagaaaacg caacagtaga
     4261 agaaaccaca gaatcatgaa aagttatttg atcatattgt ctgcaggtat gctgacattc
     4321 gcagcttcct gtgcatcggt tgaacaggaa aatgacacta gtgctttgga atcaagaatt
     4381 gattctcttg aagcagagat cgcagagctt aaaactgaac ttgctcccgg actggcggta
     4441 ctgatgaacc agaactatga ccacctcaaa aaactggagg tggccatcaa tgaagggaac
     4501 tgggaatacg cagatttttg cctgcatgaa atggacgaga cattcgaaca gatagtggat
     4561 ctgcataata accacgatga gttggtgcaa cctgcagaca cacagttcaa ggcctttatc
     4621 tttccaacgt ttgaacagtt ggaagagaaa gtggatacaa aagaccaggc aggagcatct
     4681 cagctgttca tcgaactgaa gaccaactgc agtaagtgcc atacagccaa taaccacgga
     4741 ttcatcaggc tgtaagcaga accaacaaac atggaagaaa cgaacaagcg ggaagaaggg
     4801 caggtgtacc atgtgtcggg catgtacaag gactggttcc tggactatgc ctcctatgtc
     4861 attctggaaa gggcggtgcc tgacagccgc gacggactga aaccggtgca gcggcgcatc
     4921 ctgcacgcca tgaaagagat ggatgacggt cgcttccaca aggtggccaa catcatcgga
     4981 cagaccatgc agtaccaccc gcacggtgat gcggccatcg gcgatgcact ggtcaacatg
     5041 ggccagaaag atctgctcat cgatacccag ggtaactggg gtgatgtgcg caccggcgac
     5101 tcagcagctg caccgcgata catcgaagca cgcctgtcca agttcgctct cgaagtggtg
     5161 ttcaacaagg ataccaccga atggcagctg agctacgatg gcagaaaacc ggagcctgtt
     5221 tcactgccgg taaaattccc cctcgtcctc gccatgggtg tggaaggcat cgctgtcggc
     5281 ctcagcacca agatcctccc gcataatttc tgtgagttgc tggaagccag tatcgcctgc
     5341 ctgaaaggtg aagagttcca gcttttccct gacttcgcca ccggtggttt catcgatgtg
     5401 agcaattacg atgatggcat gcggggcgga aaggtaaggg tccgtgccaa actggaagta
     5461 gccgataaaa agaccattat cattcgtgag atcccttacg gaactaccac aacttctatc
     5521 attgattcta tcctgaaagc gaatgataaa ggcaagatca agatcaaaaa ggtgacggac
     5581 aataccgcca aagatgtcga gatcctgatc gaactgcctg caggtgtgtc gccagatgtt
     5641 acggtcgatg cactctatgc cttctccgat tgtgaagtga gcatctcgcc catctgctgc
     5701 gtcattcgta aggacaaacc ggtattcgtg ccggtcagtg aactgctgaa agactctgcc
     5761 atgttcacca aaggcctgct caaacaggaa ctggagatcc gccttgctga actggaagac
     5821 aaatggcact acagctcact cgaaaagatc tttattgaga accgcatcta ccgcgacatc
     5881 gaagagtgcg agacctggga agccgtcata gaaaccatag acagaggcct ggatcctttc
     5941 aagaagaacc tgcaaaggga ggtgaccaga gatgatatcg tccggctgac ggagatcaag
     6001 atcaagcgca tctccaaata tgactccttc aaggcagatg aactcatcag atccattgaa
     6061 gcggagatca aggaggtcaa gaacaacctg aagaatctga ccaaatatac catagcctgg
     6121 ttccagggcc tgctggataa gttcgggaaa ggcagggaac gaaagaccga gatcaaggtg
     6181 ttcgatgtca ttgaagcgaa agcggtggtg gccaacaata ccaaactgta tatgaaccgt
     6241 gccgatggtt ttgttgggtt cggcctgaag aaggaggaat ttgtgtgcga atgttccgac
     6301 ctcgatgaca tcatcgtctt taccgaagaa ggtatcatga aagtggtgcg tattgccgat
     6361 aaggttttca tcggaaagaa gatcattcat gcaggtgtct ggcggaaaga cgacgaccgt
     6421 atgacctacc atgcgatcta tacggacggc aaaacaggaa agtccttcgc caagagattc
     6481 aacgttacct ctatcacccg agataaggaa tacaacatca cacagggtac tccccgaacg
     6541 aaggtctggt acttcaccgc caatcccaat ggtgaaagcg agaccgtcaa catccagctg
     6601 catcccaatg cacgggccaa gatcaagaac ttcgattttg atttcggtca actggccatt
     6661 aaaggacgaa gcgccatggg taacaccgtt accaagtatc cgctgaagaa actggtgcgc
     6721 tctgaagtgg gtagcagtac actgggcgga agaggattat ggctggatac caccgtggga
     6781 agactcaacc gcgataaccg tggtcagtac ctcggcgaat tcgataccgg cgatatgatc
     6841 ctggtgctgt tcaaagacgg aaattatgag cttacagatt ttgaactcac caaccgctac
     6901 gagatgcgtg agatagaaca ggtcagtaag ttcgatgcag atgaggtcat caccgccatt
     6961 tactacgaag gagaatccgg tgttacctac gtcaaacgat tctatgtgga ggccaccgtg
     7021 ccaggtaaaa aatacccctt tatttccgaa acgaaaggta cgcagttgat ctatgtgagc
     7081 accgaagaac aaccacgcat ctccatgacc atcctgaaag gaaagaagga agaagaggtt
     7141 accgaagaga tagacctggc atcattcata gacatcaaag gctggaagag tcagggcaat
     7201 cgcctgagtc cgcacaccat caaagagatc gcaggtctgg tcgctgaaga agaacctgaa
     7261 accgaagacg ataatgtcgg catcggtggt accatcgagt gggacctgga aaagggcgat
     7321 aagaaagtaa aaggcggtaa aggtcagggc gagttattct gaccgcagta acacatgcgc
     7381 atgactgacc gccagatctg cagtatcgaa gctggtatag gaggagtgta aaggttcttt
     7441 ttccagttgc gctttgtgcc ccggacggat ctcttccgtg atcagctgat agcctgtttc
     7501 tctgaacaac tgatggtact gcgataaccg ccacctgttt tgtggctgaa tgctgttgtc
     7561 tatacgcttc cactgtgctt cactgtaccg caggaaatga tagatgctga tgctgctgtc
     7621 catgtgcgca aagtggtctg acatgtcaat gaagtgactg tgcagaccat ccggtttccc
     7681 aatccgtctg aactctttga ggatatcccg aagaatgtca ggataaacat gctcaaaggt
     7741 gttgttggag tgaatgaatt ccaccgaatc accaggtaaa ccgctctgcc tggcatcacc
     7801 tacccgatag tcaataccca atggtgacaa catagttctg gtatcagatt tcggggcttg
     7861 aagccatgca gtaagttgtg ctttccggtc gtcctgaaag cctggttcat ccggaatttc
     7921 gtccaggatt cgccgggcag tttccagggc aaaacccggt ttgcaatggc gtgaaatatc
     7981 tgtggtcatc acattttcag caccacacag ataaaaataa aatggtacga taggatacca
     8041 gccggtacct aattcgattg tcgatgaagg acagttccgg ccggtatgtg cccggtacaa
     8101 acgaatatga tctctggcat gctggagctt atctgaaaaa tactgatcac tcagctgtac
     8161 acctttcgtc acatgacgct gaaacaggta attgagatgg tgtgatcccg gcagcaccga
     8221 taaggtcttt tgtaccagtg ccttgaacat ccactttccc cgcattgcca tcgcccaaaa
     8281 atacgggaat tgctcttgtg tctctccgga tgggtattgc ccgccctccg tcggcgtaca
     8341 agcaggggga ggggtgattg tctccctgga cggggagtac ccgcaggggg aggggtgatt
     8401 gtctccccac cactgcgcta aagaaaagca ttggttcgcc aataccccac caccatgaca
     8461 ttttagccta ttttcaggtg gtgatttata tcatttgttg tcaccgctca cgggcatttt
     8521 gcctaccttt gtacctgaat gcataaactt ctcataaagt attgacaatg aaagccgtag
     8581 gacataaaaa tccgaatgca accctggaac aatacgggtt gaaacacagt gccaccagct
     8641 actggaacct cacacccgca gagctgacag aacacagcat cagcaggggg atggccgaaa
     8701 tggccgattc cggagcactc gtggtggata ccggaaaatt caaaggccgt gcgcccaaag
     8761 acaagtacac ggtgaaggat gcgaaaaccg aaaacacggt ggactggaac aacttcaata
     8821 ttccgaccga caatgccacc ttcgatcacc tgtacaacaa ggtcatggca tacctggccg
     8881 ataaagatgt ctgggtgcgt gatgcctatg cctgcgccga tccgaaatac agactgaaca
     8941 tccgggtcat caacgaattc ccctggggca atatgttctg ttacaacatg ttcctgcgcc
     9001 ccaccgccga agagatcgac accatgacca acgactggct catcgtgaac gctcccggat
     9061 tccaggctga ccccgctact gataatgtgc gctctgccaa cttcgccatg atcgatttca
     9121 ccaaacagat catcctgatt ggtggaaccg gctacaccgg cgagatgaag aaaggcatct
     9181 ttaccgtgct caactatgtg ctgccccagg acaagggcgt gctggccatg cactgctctg
     9241 ccaacgtggg taaggatggc gatactgctg tcttcttcgg actgagcggt accggtaaga
     9301 ccaccctgag tgctgaccct gatcgcaaac tcatcggcga tgatgaacac ggctggaccg
     9361 aagacacggt cttcaacttt gaaggcggat gctacgccaa gtgcgtggat ctgaccaaag
     9421 agaaagaacc ccagatcttc gatgccatca aattcggttc tatcctggag aacatcgaat
     9481 acgtacctgg tacccgcacg gtcgatttca ccaacgtgaa gaagaccgag aacacccgtg
     9541 tcagctaccc gatcgactac atcgataaca tcatggtacc cagcatgggt ggaacaccga
     9601 agaatatctt cttccttacc tgcgatgcat cgggtgtact gcctcccatc agcaaactga
     9661 ccccgggtca ggccatgtac cagttcctga gcggatacac cgccaaggta gcaggtactg
     9721 aagcaggtgt gaccgaaccg cagctgacct tctctacctg ctttggcgcc cccttcctgc
     9781 cactgcatcc taccaaatat gcagagatgt tgggtgagaa gatgcgcaag cacaatgtga
     9841 acgtatggct gatcaatacc ggctggaccg gcggacctta cggcgtgggt aaccgcatca
     9901 agctgtcata cacccgtgcc atgatcaccg ctgctctgaa tggagaactg gataaggtat
     9961 cttataagaa acatgaggtg ttcggactta tgatgcccga aacctgtccg aatgtaccgg
    10021 atgaaaaact cgatccgcgt tctacctggg cagaccagaa agcatacgac gagaaagcag
    10081 ccaacctagc tgcgcagttc gtgaagaact ttgaaaagta tgccgacaag gccaacgaag
    10141 agatcctggc tgcagcaccc aaggtgcctg cgaatgcgtg atgatcaaaa catagttaca
    10201 aaaggtgcgg tcatttgacc gcaccttttt tttggagcaa ttcacctgct gctccccatt
    10261 aaatgtggtc gggctttccg ctaaatccct cccttgcagc attgtgagcg atcagaagca
    10321 gtgcttcaag gaaggggata ccgctgcaat cccggctatg aagcaaacta tggagcttcg
    10381 ttggagctac tgccagacga tcttttgctg acccagcacc ataccggttt catccagcag
    10441 attgatcagg tagataccgg atggggcatt gtttctgtcc a
//
LOCUS       DAOMAX010000068        10451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_860_length_10451_cov_3.690746, whole genome shotgun sequence.
ACCESSION   DAOMAX010000068 DAOMAX010000000
VERSION     DAOMAX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_860_length_10451_cov_3.690746"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2446)
                     /locus_tag="PK742_05345"
     CDS             complement(<1..2446)
                     /locus_tag="PK742_05345"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76048.1"
                     /translation="MYRLLYVCCILLIVFSTSCNRTTVQELYSGPSFEPADQFFLART
                     WPDTLFPLEAYRMAMQLAAQDRDEHLSGTRGGGADWMEEGPYNISGRFNTIAIHPDNS
                     EIILAGLASGGIFKTTDGGDTWYPVFDDFAYLAIAHILYDPVDPDIVWAATGDPNISG
                     FPFIGDGVYKSLDGGETWTYSGLSEAGILSKVAIAASDHNTIYVGSMGIPFERNEDRG
                     LYKSVDGGTTWTKVLFVDDDCGVIDIALHPADPDRIFVAVWNRIRNNHESLVAGPDAG
                     VYRSLDGGDTWTKLTGGLPGGNLSRISVEMDPDNPETIYASVVDDSYSLEGIYKSENN
                     GATWNDLPLVSGVSGVMSGFGWYFDGIQLNPFETNELFVLGVGLYRTTNDGTTWNDKG
                     GSTHADKHAMAFAGASDYWLATDGGLYQTSSDASVWNDGEDINSAQFYHIDQNPHEPG
                     VYWGGMQDNGTSRGSDLTGGLWEDIYGADGFHSEFRTDDEDKFYVEWQNGGIEAFNGF
                     DFWDATEGISSSDRRNWNMPYMISRFDNDVLYAGTYRVYKSTVGYLPDYAPISGDLTD
                     GNIFGSAFHTLTVIEEDRFDPLVLYTGSVDANVYRTLNGGATWNNVTGTLPEQYVTDL
                     VASPSTPDRVWVTHSGYKEYDFLPHIHRSDDNGTTWTNVSGDLPPLAINAVAVHPEND
                     DMLFVATDGGVYYTLNGGSSWNRLGNNMPVIAIYDIEYDAEHNKVLAGSYARGLWTFD
                     VASIIPPVISGLADTLVCAGDILTLTATGASTYFWTSDEAEIICAGTCETAEIIATTD
                     ATIEVIGTNDYGLSDTI"
     gene            2533..3420
                     /locus_tag="PK742_05350"
     CDS             2533..3420
                     /locus_tag="PK742_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQU76049.1"
                     /translation="MTRLSTIGAFSLLSWLLLPVSAQAQQQNSLLWKVEGKSLTSPSY
                     IYGTIHYIGKDDFFLRDEVTDALSTTASSVFEIALDDQEEMSTLTGLLMLPPGTYISD
                     FVTPEEYERIDRYLMDSLGQSIMLYRSMKPFVLGQSITAGRSFGTETESYEMVLMEMA
                     QTNNKKIYGLESVADQVGFMDSIPYDVQLDWTLEALDHPGEADTVFPALVRYYKAEDL
                     EGMLQCMLHASPEIQDYGDLLLYNRNATWIPVMEDMMEEESRFFAVGAGHLPGPKGVL
                     TLLREAGYTVTPVNNKTHD"
     gene            3413..4192
                     /locus_tag="PK742_05355"
     CDS             3413..4192
                     /locus_tag="PK742_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU76050.1"
                     /translation="MIEVQNLSKTFRLTKKQMKESGHSGDPEVLAVQDVSFRCLPGRI
                     FTLLGPNGAGKTTTLRMIATILKPTEGHITVAGFDTVHQGAEVRARLGFLTGSTGLYD
                     KLSPNELIDYFGRLNNMDEQTIARRKDELFTLLGIHEFANRRIGRFSTGMKQKVSIAR
                     TMIHDPEVIVFDEPTSGLDVIAAKGIIDLIRNSKLQQKTVILSTHIMSEVNMLSDDLA
                     IIHKGRMIYNNTYEQFQAGMQAATLEDEFIRLATASETATL"
     gene            4196..5404
                     /locus_tag="PK742_05360"
     CDS             4196..5404
                     /locus_tag="PK742_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU76051.1"
                     /translation="MNNIITIFRKELKEILRDKRTLIIMLVLPLVVYPLLFTGVSSFM
                     QGQAEKAGEETLRIGLGAGGDAALFREYLSAWPGKTDVRSISSDTAEAKVQIQADSLD
                     VVFFFPDGFTASVDSLDPVSYTYYYTSTNKEFQVDMIRGISQGFQQSLTARKLAQLSV
                     NERVLEPAQAEAHNIASNREQFGSIIGGIIPYFFIIFCLIGCMYPAMDIAAGEKERGT
                     LETLLVSPASRLDIYLGKFMAVGLSGFISAMAAIAGILISSKMISAEAGAEASGIMEM
                     MEGIIQPGSVLLLLGILLPLNAFFAAITLMLSIYARTYKEAQGMISPMMIVTVFPSVA
                     GMLPGVTLNYVTALVPILNVSLGAKEIISGTAETGPLVVTYISLIAFALLALMASVRF
                     FNQEKNIMRG"
     gene            5780..6259
                     /locus_tag="PK742_05365"
     CDS             5780..6259
                     /locus_tag="PK742_05365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU76052.1"
                     /translation="MTTSLLWKVRSFHQLNVFELQAIYRLRQEVFVVEQQCAYLDADG
                     KDQFADHIFGVDDDGRVMAYCRVVQPGISCSEVSIGRVVTHPELRHTGLGKSVMERAF
                     KCVDDRYGKVPVRIEAQLYLKAFYEQQGFEPVGDVFLLDGIDHIEMLRPAEGQFSRV"
     gene            6354..6764
                     /gene="mscL"
                     /locus_tag="PK742_05370"
     CDS             6354..6764
                     /gene="mscL"
                     /locus_tag="PK742_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQU76053.1"
                     /translation="MGMMKEFKEFAMKGNLVDIAVGFVMGAAFAKVTSSFINGIVMPF
                     IGLIQGKDMSEWKYVIKEGTAGADGAVTGEVAVLYGTFISVVIEFIVVAFVMFLVIKG
                     INAMKKKEEAAPAAPPAPSKEEVLLGEIRDLLKK"
     gene            6844..7791
                     /locus_tag="PK742_05375"
     CDS             6844..7791
                     /locus_tag="PK742_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQU76054.1"
                     /translation="MKKMMLTLVAGLMTTCMFAQTDTSWTRGGSATLNFNQIGLTNWA
                     AGGENAFSSQLLTGFFANYKKDKVTWDNYLIANYGLITANNYADIRKNDDRLELNSKY
                     GQYAFGDFYYAAQLNFLTQFTDGYDYLTSDVIPISTIMAPGYLTGALGMDWKPNDKFS
                     LFLSPATGKFTFVLDDSIANAGTYGNNAAVYDELGALITPGEKMRAEFGASLIATLAT
                     TLSDNISTQGRLTLFNNFTDPVKENRGNIDVNFVNTFSMKAGKFITLSLYLESIYDHD
                     IKIPTYDENDNVIGEGPKTQWKEVFGVGLTYRLGDPKPE"
     gene            complement(7955..8929)
                     /locus_tag="PK742_05380"
     CDS             complement(7955..8929)
                     /locus_tag="PK742_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQU76055.1"
                     /translation="MSKGPISQFITHHYRHFNAASLVDAAKGYEAHLAEGGKMMVTLA
                     GAMSTAELGVSLAEMIRQGKVDIISCTGANLEEDIMNLVAHTHYERIPHYRDLTPQDE
                     WALLEKGLNRVTDTCIPEEEAFRKIQHHIEKIWKDAEAAGERYFPHEYMYKLLLSEVM
                     KDDYEIDPKNSWMIAAAERNIPIVVPGWEDSTMGNIFASYCIKGELNARTMKSGIEYM
                     VWLADWYRNNSGGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWGYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIHTPKYIIESDATIVAPLIFAYLLGW"
     gene            9116..10258
                     /locus_tag="PK742_05385"
     CDS             9116..10258
                     /locus_tag="PK742_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQU76056.1"
                     /translation="MLNKKIILKRQEHQGVSVLMLYFPYDRQLIELSKSAGARYSQTN
                     KAWYVPDNKEQLNRLFEVFRDVAWLDIQDLRKKKVRNNRLATGKNASAILPPLSQENL
                     QHLDRFCKWMEHRNYSKLTTKSYLECVRTFFRFHNNLLPKDVTKMHLVAFNNGYILKN
                     KFSVSYQNQFVNALKLFLKETFSLPIPEEDLERPRKEKRLPNVLSREEVAILLKKTPN
                     IKHKLLLSITYACGMRRGEVLELKLSDIDLKRQVMYVRLGKGKKDRMIPLPESIRLQL
                     PVYFRAYQPTLYLFEGTESGKPYSEGSFQSVMKQALERCGIKKKVSLHTLRHSYATHL
                     LEAGTDLRYIQELLGHSSSKTTEIYTHVSTRKLSEIRSPLDDMQID"
BASE COUNT         2754 a         2513 c         2599 g         2585 t
ORIGIN      
        1 tgatggtatc agataatcca tagtcattgg taccgatcac ctcgatggtg gcatcggtag
       61 tggcgatgat ctctgctgtt tcacaggttc ctgcacaaat gatctctgct tcatcgctgg
      121 tccagaagta ggtggaagct ccggtagcgg tcaacgtcag gatatcgccg gcacagacca
      181 aagtatcagc caggccactg ataacaggtg gtatgatgct ggccacatca aaggtccaga
      241 gaccccgggc ataagagcct gccagcactt tattatgctc agcatcatac tctatatcat
      301 agatggcgat gaccggcata ttattcccca gcctgttcca gctgctgcca ccgttgagag
      361 tatagtacac gccaccgtct gtagcaacga acagcatgtc atcgttttcc ggatgcactg
      421 caaccgcatt gatggccagc ggaggcagat cgccactcac attggtccag gtggtgccat
      481 tgtcatcgga acgatgtata tgtggtaaaa aatcatattc tttgtaccca ctatgtgtta
      541 cccatacacg atcaggagtg gaaggtgatg caaccagatc ggtcacgtac tgttccggaa
      601 gagttcctgt cacgttattc caggtagcac ctccattgag ggtacgatag acgttggcat
      661 caacagaacc tgtgtataat accagggggt caaagcggtc ttcttctata acggtgagtg
      721 tatgaaatgc agagccgaaa atattgccat ctgtcagatc tccgctgatg ggtgcatagt
      781 ctggcaaata cccgactgta cttttgtaca ccctgtaagt gccggcatac agcacatcat
      841 tatcaaagcg gctgatcatg tagggcatgt tccagttcct tctgtcgctg ctgctgatac
      901 cttcggtagc atcccagaaa tcaaacccat taaaagcttc aatgccgcca ttctgccatt
      961 ccacataaaa tttgtcttca tcatctgttc tgaattcaga gtgaaaacca tcggcgccgt
     1021 agatatcttc ccagagccca ccggtcaggt cgcttcctct gctggtgcca ttgtcctgca
     1081 ttccgcccca gtacactccc ggttcatgcg gattctgatc gatatgatag aactgagcag
     1141 agttgatgtc ttctccatcg ttccagacag acgcatcact gctggtctgg tagagaccgc
     1201 catcagttgc cagccagtaa tcggatgctc cggcaaatgc catagcatgt ttatcagcat
     1261 gggtacttcc ccctttatca ttccaggtag taccatcatt ggtggttcgg tacagtccaa
     1321 cgcccagtac aaagagttca tttgtttcaa acgggttgag ctgaatgccg tcaaagtacc
     1381 agccgaatcc actcatcaca ccactgacac ctgataccag aggcagatca ttccaggtag
     1441 cgccattatt ttctgacttg tagattcctt ccagagagta gctgtcgtcc accacagagg
     1501 catagatcgt ttcagggtta tcgggatcca tttccacact gatgcggctc aggttaccac
     1561 cgggcagtcc gccggtcagt tttgtccagg tatctccccc atccaggctc cggtaaacac
     1621 cggcatcagg gccggccacc aggctttcat ggttatttct gattcggttc cagacggcta
     1681 cgaagatgcg gtcagggtct gcagggtgca gggcgatatc gatgactccg caatcgtcgt
     1741 ccacaaacaa cactttggtc catgtggttc caccgtccac cgatttatag agccctctgt
     1801 cttcattccg ctcaaaagga atacccatag aacccacata aatggtattg tggtcgcttg
     1861 ctgcaatagc cactttagat aatataccgg cttcagaaag tccgctgtag gtccaggttt
     1921 cacccccatc caggcttttg taaactccat ctcctatgaa aggaaatcct gaaatattcg
     1981 gatcgccggt ggcagcccat acgatgtcag gatcaaccgg atcatagaga atgtgggcaa
     2041 tggccaggta ggcgaaatcg tcgaacaccg gataccaggt atcaccgcca tcggtcgttt
     2101 tgaagatgcc cccgctggcc agtcctgcca ggatgatctc actgttatca ggatgaatgg
     2161 cgatggtatt gaacctgccg ctgatattat aggggccttc ttccatccag tctgcaccac
     2221 cacctctggt tccggacaga tgttcatctc tgtcttgtgc ggccagctgc atggccattc
     2281 tgtatgcctc aagggggaac agggtatccg gccaggtgcg ggccagaaaa aactgatctg
     2341 ccggctcaaa agacggacct gagtacagtt cctgcacggt tgtgcggtta caggaggttg
     2401 aaaatacgat caacaagatg cagcagacat aaagaaggcg gtacatagct taattttaaa
     2461 aagcttaaaa ataattaaca tctgccctat cgccatactg tgtagttttg ggcgtttcct
     2521 tgtgacggag ttatgaccag actatcaact atcggtgcct tcagcttatt atcctggctg
     2581 ctgctgcctg tatctgcgca ggctcagcag cagaacagtc tgttgtggaa ggtggaaggc
     2641 aaaagcctta cttcaccatc gtatatatat ggcaccattc attacatcgg gaaagacgat
     2701 ttctttttac gtgatgaggt gaccgatgca ctgagtacaa ctgcctcttc ggtattcgag
     2761 atcgccctgg atgaccagga agaaatgtcc accctgaccg gtctgctcat gctgccaccg
     2821 ggcacctaca tctcagattt tgttacgccg gaagagtacg agcgaataga ccggtatctc
     2881 atggacagcc tggggcagag catcatgctg taccgtagca tgaaaccctt tgtgcttggc
     2941 caaagcatca ctgccggaag atcattcgga acagaaacag aatcttacga gatggtactg
     3001 atggaaatgg cgcaaacgaa caacaaaaaa atatacggtc tggagtctgt agcagatcag
     3061 gttggcttca tggattccat accctatgat gtacagttgg actggaccct ggaagcactg
     3121 gatcatccgg gagaagcgga cacggtcttt ccggctcttg tcaggtatta caaggccgaa
     3181 gatctggagg gcatgctgca gtgcatgttg catgccagtc cggaaataca ggactacggc
     3241 gaccttcttc tgtataacag aaatgccacc tggataccgg tcatggaaga tatgatggag
     3301 gaagaaagca ggttctttgc ggtgggagca ggccatcttc ccggacccaa aggcgtatta
     3361 acattactgc gtgaagcggg ttataccgtg acacctgtca ataacaaaac acatgattga
     3421 agtacagaat ctgagcaaga ccttccggct cacgaagaag cagatgaagg aaagcggcca
     3481 ttccggcgat ccggaagtgt tggcagtgca agacgtgagt ttccggtgtt tgccgggacg
     3541 aatcttcacc ctactggggc caaatggtgc aggaaagacc acgactctgc gcatgatcgc
     3601 aaccattctg aaacctactg aagggcacat cactgtggca ggatttgaca cagtgcatca
     3661 aggtgctgaa gtacgtgcca ggcttggttt cctgaccggc agcacaggat tgtatgataa
     3721 actgtcgccc aatgagctga tcgattattt cggcaggctc aacaatatgg acgagcagac
     3781 catcgcccga agaaaggatg agttgttcac cctgctcgga attcatgagt ttgccaacag
     3841 gcgcatcggg cgattcagca ccggaatgaa gcagaaggtg tccatcgcac ggaccatgat
     3901 acacgacccc gaggtcatcg ttttcgatga acccacgtca ggtctggatg tgattgctgc
     3961 gaagggtatc atcgacctta tccgcaacag taaactgcaa caaaaaacgg tcattctgag
     4021 tacccacatc atgagtgaag tgaacatgct gagcgatgac ctggccatca tccacaaagg
     4081 ccggatgatc tataacaata catacgaaca gttccaggca ggcatgcagg cagctacact
     4141 ggaagatgaa ttcattcgcc tggccaccgc ctcagaaacc gctaccctat aagccatgaa
     4201 caacatcatt accattttca gaaaagaact gaaagagata ctgcgagaca agcgtacact
     4261 gatcatcatg ctggtgttac ctctggttgt atatccactg ctatttacgg gtgtttccag
     4321 cttcatgcag ggacaggcag aaaaggccgg agaagaaaca ctgcgcatcg gattaggcgc
     4381 aggcggcgat gctgccttgt tcagagaata tctctcagcc tggccgggga agaccgatgt
     4441 gcgcagcatc agttcagata cagccgaagc aaaagtgcag atccaggcag acagtctgga
     4501 cgtagtgttc tttttcccgg atggcttcac agcgtcggtt gacagtcttg atcctgtcag
     4561 ttatacatac tactatacct ccaccaataa ggaatttcag gtggacatga tcagaggtat
     4621 cagtcagggg tttcagcagt cgctcactgc ccgcaagctg gcccagcttt cagtcaacga
     4681 acgggtactg gagccggcac aggcagaagc gcataacata gcgagtaacc gggagcagtt
     4741 tggtagtatc atcgggggca tcattccgta tttcttcatc atcttctgcc tgataggctg
     4801 tatgtatccg gccatggata ttgcggcagg tgaaaaagag agaggcacgc tggaaacgct
     4861 gctggtttcg cctgcttcca gactggacat ttatctgggt aaattcatgg cagttggatt
     4921 gtctggtttc atctcagcca tggctgccat tgcgggcatt cttatatcga gtaaaatgat
     4981 atcagctgaa gcaggtgcag aggccagcgg tatcatggag atgatggaag gtatcatcca
     5041 gcccggcagt gtcttgttgc tgctgggtat cctgctgcca ttgaatgcat tctttgctgc
     5101 cattacgctg atgctgtcta tttatgccag aacctacaaa gaagcccagg gcatgatcag
     5161 cccgatgatg atcgtaaccg tttttccttc ggtagccggt atgctgccag gcgtcacact
     5221 caattacgta acagcactgg ttcctatcct gaatgtatca ttgggtgcca aagagatcat
     5281 ttccggcacg gcagagaccg gtccgctggt ggttacctat atcagcctga tagcattcgc
     5341 tttgctggcc ctgatggcca gtgtgcgctt cttcaaccag gagaagaata tcatgcgggg
     5401 atgaggggaa tgcagttggc agtttgcggt ggacagttgg cggttaggaa tggagcagta
     5461 caatgcggaa tacggagtgc ggaaggcgaa gtgtggaatg cagttggcaa ttggtggttt
     5521 ggagtcgagc agtgagcagt aaagagtgag gagtgagcag tacaatgcgg aatacggagt
     5581 gtggaagtcg aagtacggaa tgctgttggc agtatattga gaggtgagaa gtgagaggtg
     5641 aatggtgaat accactaccc gatacctgat actcgatacc tgatactttt tacccacacc
     5701 ccatttattc acagaaccag ccattttatc aacaaaactt tcgggcaaaa atgtcgttat
     5761 ataatttgga atcagaaata tgaccacaag tctgctttgg aaggttcgca gttttcatca
     5821 gctcaatgta tttgagttgc aggcgatcta tcggcttcgc caggaagtgt tcgtggtgga
     5881 acagcaatgt gcctatttgg acgctgatgg caaagaccag tttgccgatc atattttcgg
     5941 agtggatgat gacggacgtg taatggctta ctgccgcgta gtacagcctg gcatcagctg
     6001 ctctgaggtt tccatcgggc gagtggtaac acatccggaa ctgcgccata ccggtctggg
     6061 taaatcagtc atggaacggg cattcaaatg tgtagatgat cgctatggca aagtgccggt
     6121 gcgcattgaa gcacagttat acctcaaggc tttttacgag cagcagggct ttgagcctgt
     6181 gggcgatgta tttctgctgg atgggataga ccatattgaa atgctgcgtc ctgcagaagg
     6241 tcagttctcc cgtgtctgac ctgttgcgtt ttccacctga cattttcttg acaatatcca
     6301 attttccgcc atatttgtgg tgaatctgtt ttttcaccga aaaaccaaca actatgggaa
     6361 tgatgaagga attcaaggag tttgccatga aaggcaatct cgtggacatc gccgtgggct
     6421 ttgtcatggg tgccgctttt gccaaagtga cctccagttt tatcaatggt attgtcatgc
     6481 cattcattgg cctgatccag ggcaaggaca tgtctgaatg gaaatacgtg atcaaagaag
     6541 gaaccgcagg cgcagatggt gctgtaaccg gcgaggtggc tgtactctat ggtacattta
     6601 tctcagtagt gatagaattc attgtggtgg cctttgtgat gttccttgtt attaaaggca
     6661 tcaatgccat gaagaagaaa gaagaagctg ctcctgccgc acctcccgct ccaagcaaag
     6721 aagaggttct gttaggtgag atcagagatc tgctgaaaaa ataacaacac tcccctgccg
     6781 gcagccacct gtgctgccgg catttttcct gatcacacac acaggaattt taaaacactg
     6841 actatgaaaa aaatgatgct gaccctggtg gcgggtctga tgaccacctg tatgttcgca
     6901 cagaccgata cttcctggac cagaggcgga tcggcaaccc tcaacttcaa ccagatcggc
     6961 ctgaccaact gggcagcggg tggtgaaaat gctttcagca gccagttgct tacaggtttc
     7021 tttgccaatt ataaaaagga caaggtgacc tgggataatt acctcattgc caactatggt
     7081 ctgataaccg ccaataatta tgcagacatc cgcaagaacg acgaccgcct ggaactcaac
     7141 tccaagtatg gacaatatgc cttcggcgat ttttactatg ctgcgcagct gaactttctg
     7201 acacagttca ccgatggtta tgactacctg acaagcgatg tcattcccat ttccaccatc
     7261 atggcccccg gctacctgac aggtgcattg ggtatggact ggaaacccaa cgataagttc
     7321 agcttgttcc tctctcctgc caccggtaag ttcacttttg tgctggatga cagtatagct
     7381 aatgccggta cgtatggaaa caatgcagct gtatatgatg aactgggagc actcatcaca
     7441 ccgggagaaa agatgcgggc agaatttgga gcgtcactga tagcaaccct ggctactacc
     7501 ctctctgata atatctccac ccagggaaga ctgaccctgt tcaacaactt taccgatccg
     7561 gtaaaagaga acagaggcaa tattgatgtc aacttcgtca acaccttcag tatgaaggcc
     7621 ggcaagttca ttaccctgag cctgtatctg gaaagcatct acgaccacga catcaagatc
     7681 ccgacctacg atgagaatga caacgtgata ggagaaggac ccaagaccca gtggaaagag
     7741 gtgttcggtg tgggactgac ctatcgtctg ggtgatccga aaccggaata gggatagggc
     7801 ggaacactga tcaaaccgat tgaactgata taacagatca aaaaaaggcc ggaatctccg
     7861 gccttttctc attaagatat gtcaaatcag ttatatccct gcttgcagca ggcaagtgtg
     7921 ttccatgcct aacggacagg aaggggccca gacatcacca acccagcagg taggcgaaga
     7981 tgagcggagc aacgatcgta gcatcgcttt caatgatgta tttcggcgtg tggatgtcca
     8041 gcttgcccca agtgatcttc tcattgggta ctgccccgga ataggaacca taagaggtag
     8101 tactgtcgct gatctggcag aagtaacccc agaaaggcac atcatgccat tccaggtcct
     8161 ggtacatcat aggcaccacg cagatgggga aatcgccggc aatgccccct cctatctgga
     8221 agaaacctac gccttttccg cctgagttgt ttctgtacca gtcggccagc caaaccatat
     8281 actcgatacc gctcttcatg gtgcgggcat tcagttcacc tttgatgcag taggaagcaa
     8341 agatgtttcc catggtactg tcttcccatc cgggtacaac gatgggaata ttgcgttctg
     8401 ccgcggcgat catccaggag tttttggggt cgatctcata atcgtccttc atgacttcgc
     8461 tcagcagcag tttgtacatg tattcgtggg ggaagtaacg ttcgccggca gcttctgcat
     8521 ccttccagat cttctcaatg tgatgctgga tcttgcggaa tgcctcctct tccgggatgc
     8581 aggtatcggt tacccggttc agtccttttt ccagcagagc ccattcgtcc tgcggggtca
     8641 gatcgcggta gtggggaata cgctcataat gcgtatgcgc taccaggttc atgatgtcct
     8701 cttccaggtt ggcgccggta caggagatga tgtccacctt cccctgacgg atcatttcgg
     8761 ccagcgatac gcccaactca gccgtgctca tggcaccggc cagggtaacc atcattttgc
     8821 ctccttcagc caggtgggct tcatagcctt tggcggcatc caccagcgaa gcagcattga
     8881 aatgacggta gtgatgggta atgaactgcg atatgggtcc tttggacatg gcgatgggtt
     8941 ttgggcaaag atagttcgtt gtgtgtaatg cgttgtgcgt taatcgttat tcgttgttcg
     9001 ttgttcgtcc cgttacacta tacctgatac ttgctgccat ttctcatgca ggcctgaccg
     9061 ttgcagctga tgatatacaa ctgtcttcta tatattagca ctagcttgac ccgctatgct
     9121 gaacaaaaag atcatcctga aacggcagga acaccagggt gtgtccgtgc tgatgttgta
     9181 tttcccatat gacaggcagc tcattgaact gagtaaatct gccggagccc ggtattccca
     9241 aaccaataag gcatggtatg tgccggataa taaagagcag ctgaacagac tgtttgaagt
     9301 gtttcgtgat gtagcgtggc ttgatataca ggatctgcgg aaaaagaaag ttcgcaacaa
     9361 cagacttgca acaggtaaaa atgcgtctgc aatactaccc ccactttcac aggagaatct
     9421 gcagcatctg gaccgttttt gtaaatggat ggaacacagg aattacagca aactgaccac
     9481 taagtcatat ctggaatgtg tacgaacctt tttcagattt cataacaacc tgttaccaaa
     9541 ggatgtaacc aaaatgcacc tggtagcatt caataatggc tatatcctga aaaacaaatt
     9601 ttctgtcagt tatcagaatc aatttgtcaa tgcactaaaa ctattcctga aagagacctt
     9661 tagcttacct ataccggagg aagatttgga acggccccga aaagagaagc gattacccaa
     9721 tgtactttcc agggaagaag tagcgattct attaaaaaag actccgaaca tcaagcataa
     9781 gctcctgctg agcattacct atgcatgtgg gatgcgcaga ggcgaagttc tggaactcaa
     9841 actatcagat atagacctga agaggcaggt catgtatgtc aggctgggaa aaggcaagaa
     9901 ggacagaatg attcctttac ctgagagtat ccggttgcag ttacccgtat atttccgggc
     9961 ctatcaaccg accttgtacc tttttgaagg cacagaaagc ggcaaaccct attcagaagg
    10021 cagttttcag agtgtcatga agcaggctct ggaacgatgt ggaataaaga aaaaagtcag
    10081 tttgcataca ctcaggcaca gttatgcgac gcatcttctg gaagccggaa ctgatctgag
    10141 gtacattcag gaactcctgg gccatagcag cagcaaaact accgagatct acacccatgt
    10201 cagcactcgg aaactaagtg aaatacgctc cccactggac gatatgcaaa tcgattaaag
    10261 ttgactaatt ttaacattta ctgtagatat ccctcccaat aaggatatat ctgacaaaaa
    10321 actactgata taaacaagtt atagggcatt ttaaaagact tcactaacaa atgaaattat
    10381 taggccttac aatatcattt ttacttcttt ttacttcttg taaggagaca aacaaagaag
    10441 cttttgactt a
//
LOCUS       DAOMAX010000069        10395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_867_length_10395_cov_4.258994, whole genome shotgun sequence.
ACCESSION   DAOMAX010000069 DAOMAX010000000
VERSION     DAOMAX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10395
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_10395_cov_4.258994"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PK742_05390"
     CDS             <1..244
                     /locus_tag="PK742_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76057.1"
                     /translation="VHFIQSLEQKFGLELFNRMLLTYKDEIGLHTITLSELHDALDDG
                     KLSMDTIVANPLVATKQEFDAGFFAPLKDTWVANMV"
     gene            complement(252..1541)
                     /locus_tag="PK742_05395"
     CDS             complement(252..1541)
                     /locus_tag="PK742_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQU76058.1"
                     /translation="MKKGLLFAFMLAGLRLFGQSYDLAITEIMYNPDSSLNGQDWVEL
                     YNYGTTQIDISGWLLKSEDVLDEFEIPDGTILQPGEFKVIAQWEDTFHMVYPTVSNVI
                     GDFEFGLDNGGGQVRLFNGGGAPVIQISYGDTLPWPKSADGYGMSLEVDDYTAELNDP
                     SNWFGGCVGGSPGSEYSDCNYSVQLSEINYNSIFYHDSGDWIELVNSGIGAIDISDWS
                     IRDSKDNNVFHIPAGTILEAGAHLVVCTDIFQYTTYYPAIDNLIGDLGFGFSGQGDAV
                     RIYDNEGILQYGLYYHDDPPWADEADGNGFTLELLDFYGTPNVPGAWTAGCPYGSPGT
                     AIILPCPAAVEDYELLPFTAGPNPFNTALYLRSETVHNGLITITDLQGRPVYSTPWNG
                     SLVWNGQNGSGEEVASGLYLVRLQTDGEQWSMLVVKE"
     gene            complement(1627..2325)
                     /locus_tag="PK742_05400"
     CDS             complement(1627..2325)
                     /locus_tag="PK742_05400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQU76059.1"
                     /translation="MRNLLFIAIAVAVFSSCQKSGTAQSVELKTENDSVSYALGVNIG
                     ESLKKQGAGDLNFELMLTMAERAYAGDTTLQMNEQECMNYLNTYFQRKQMEAANATME
                     EGKQFLAENAKKEGVMTTPSGVQYKILKSGDANAPSPVMGDKVLVNYTGTFINGEEFD
                     SNKSKGAPATLTLGQVIQGFNEILQMMHEGDHWMVYVPSEQGYGSRTGGRIPANSVLV
                     FDLELVQVVPAPAK"
     gene            complement(2370..2801)
                     /locus_tag="PK742_05405"
     CDS             complement(2370..2801)
                     /locus_tag="PK742_05405"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQU76060.1"
                     /translation="MYVLDEQEMEPDYSFAGFGIRAVAYLIDAGILALVRLFVSMLFG
                     VSFLSTGNNIIWFGSLFGLLYFILLESGARRGTIGKQLLGLQVTDESGHRISLGQSVI
                     RNLMKIVSSLILGVGFLMVILDERKRSLHDRAAKTYVVSVR"
     gene            complement(2822..4156)
                     /locus_tag="PK742_05410"
     CDS             complement(2822..4156)
                     /locus_tag="PK742_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQU76061.1"
                     /translation="MIRFWQHITLRNRFFWWLGGLIILYTVSFFIPFLYPVAIVAMLF
                     FAALTLVDAALLFIVKQPLSVSRRLPQVMSLHDENEVHLHITGRFPAGLRLRIIDELP
                     QQLQKRDLELQYRLPARTSRELTYHLRPLERGAYHFGKVHIFISYLFGLAERKVSIDL
                     EDMVKVYPSVMQMKKFELYTIRRIAHFQGMKKMRRIGHSYEFDQIKQYVPGDDVRSLN
                     WKATGRSASLMVNHYEDEKAQQVYCLIDKSRTMKTPFDGLSLMDYAINTSLVISNIAV
                     RKEDKAGIITFASKVNTVLRADRHAGQMRKILELLYNEKAIPEESSFEHLYYQVRRHV
                     HVRSLLFLYTNFESYYAIERALSVLRKLNKLHLLVVVFFENEEINRYGDQDAGNTESV
                     YLTTFAKRFTLEKQLIMQELNKYGIQTIITSPSDLTIHTINKYLELKSRGLA"
     gene            complement(4153..5118)
                     /locus_tag="PK742_05415"
     CDS             complement(4153..5118)
                     /locus_tag="PK742_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQU76062.1"
                     /translation="MENANSQVEQLRQVMDAVRAEIGKVIVGQEGLSELLMTGLLSGG
                     HMLIEGVPGIAKTLTARMLAKTIRADFSRIQFTPDLMPSDVIGTPVFNQQKSIFEFRK
                     GPVFSNIVLIDEINRAPAKTQAALFEVMEEQQVTADGTTYVLSFPFLVVATQNPIEQE
                     GTYKLPEAQLDRFLFRIRIGYPSEQEELQILQRFKQDLTTRVQETVSSVVSPDVLKQL
                     RQVVESVTISDQLLQYIASLIHLTRNHPDLFLGASPRASLAIMRTSRAYAALQGRDFV
                     IPDDIHRVAYPVLNHRIILTPDKELEGVSTEDVIRQMLEKVEVPR"
     gene            complement(5118..6395)
                     /locus_tag="PK742_05420"
     CDS             complement(5118..6395)
                     /locus_tag="PK742_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76063.1"
                     /translation="MNRGTVIAIVVGVTIATGFVIWMVRNFNSHNYFWYETYRPSSDQ
                     PYGLSYTVDLLEHFQDEKPEWIRKDLITALEQTQAPNYLFIGEKPLYTDEMADALIAY
                     ISKGNHAMIAANEFPVTLMGKLFNDGTEAGNVLPPFNEADWYQDTNTGTAILNSYHDK
                     YFDLYFRYPGLPKDRFRFSYRVRDQKESHSWRYFQTDTGGLQVAAHDDRHYPSMLDLT
                     IGNGHLYLFCNPVLLTNIYAVDSNKIPFFNIMFSHLEQGGILLDEYSKIPPSQQAGDP
                     EFNSPLRFILSQPSLRSAWYMLLAGAFLFVLFRGRREQQVIPLAEPNVNRSLEFAMTV
                     GRMHYLRGDHLHLVQQQMRLWRSFVKDKYQLVAGEDQELFISKLSMKSQVPESAIRDI
                     LDTYKSFKDRLSLKEKEMTDFYTKTRTFYKSCQ"
     gene            complement(6392..7048)
                     /locus_tag="PK742_05425"
     CDS             complement(6392..7048)
                     /locus_tag="PK742_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HQU76064.1"
                     /translation="MRHLFNILLLLAGLFSLFAAQAENGFQYREQDTRQWSETVDGEQ
                     FREATPDTEVPEMQEVPVRTISTGWLQILGYTLIIGILAVIIFYLLRSRIAGNRRVAA
                     GDEIRIEDLDQRPMESDLDRLLREAVRNEDWRLAFRIQYLLVIRALHDTGRIIWKREK
                     TNHDYVRELGLHPLADDLHTESLVFDRIWYGLRPMDHQGYLEHSNKLQRLIQRMEAGE
                     "
     gene            complement(7032..8831)
                     /locus_tag="PK742_05430"
     CDS             complement(7032..8831)
                     /locus_tag="PK742_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HQU76065.1"
                     /translation="MKETTFIDQNKAKWREFEEEFKSPQGDPEKLTNLFVQITDDLSY
                     ARTHYPHRSVRIYLNRIAQQVYFSIYKNRLNRWNKFKHFWSDELPEVMHHSRRELLFS
                     TVIFLLALGVGVLSSIHDGEFARLILGDSYISMTEENIRSGDPMKVYKESQPNDMFLA
                     ITTNNLKVAFFSFILGVTFGMGTLYILLYNGIMVGAFQYFFVERALFRESFLTIWIHG
                     TMEMSAIVIAATAGFIIGRSLLFPGTFSRLASFRQGGVRAMKVFMGIIPIIITAGFLE
                     SYFTRHTDTPDLIRALIIFAEFAFMITYYVIYPARRARKGFQTEQRPDELLPVILPEV
                     KFDRVLRESEVYTGTFSLFRRHLKALLTWSISFAALVTLAGFFDHSYMALNPFRKLYL
                     GELHDLISPGNSLTVFCLHVLSMAFVMHLSVALVFRTRSGEGNFRQLFTLRLLLPALL
                     AGSIWALLFLMPLTASTVLQMLLLPIPVYLICAWQLKRTGERLQPFIPLSRQYGSILM
                     VVAVLFITVFILMLLLDTSVSYFYSELLQWMFSDSFSMKGRIISAIMQMITLASYYLL
                     FSLIVFGLSLMFFSGKEIVSAGTLKAQIDEAFI"
     gene            8863..9588
                     /locus_tag="PK742_05435"
     CDS             8863..9588
                     /locus_tag="PK742_05435"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQU76066.1"
                     /translation="MNSIDITTSQQVVITYELASVRDRILAFFLDTIVLLGLYLGIFF
                     IFGVIGGMQDSNLFYWVAFLVMSFYSLFWELLLNGQSPGKRSLGIQVIHVNGLEPSLN
                     SYLLRWAFRTIDIWFTLGSVAVMSISAGARGQRLGDIVANTTVVRTANRQDVRMEQLE
                     QLNSRSNYEPKYTGVHAFREEQMLLVKEVLDRYLKYRNTAHREALEMTAAKVAEQLGL
                     EKIPDDLVEFLKRVIKDYVAVSR"
     gene            9788..>10395
                     /locus_tag="PK742_05440"
     CDS             9788..>10395
                     /locus_tag="PK742_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76067.1"
                     /translation="MRSRIFLLIPLVCLLHYSLQAQVAASITNREYTRLEILEDLDFL
                     EQHLTEKHPGLYEYTDSGFVVRWMDSLRQALPEKLSEQQLYADISRLCHLIGDGHTML
                     FPSSTTTAYYNAYELFFPMRTFWDGEHLYTYMHYSDADTIADGTRILYINGIPAEEVV
                     QFCLDRITHDGYNKTYPVWVLNNWFAEFYGYFYGHPSSHTVV"
BASE COUNT         2592 a         2568 c         2580 g         2655 t
ORIGIN      
        1 cgtccatttc attcaatcac ttgaacagaa attcggcctg gaattattta accggatgct
       61 gctgacctat aaggacgaga taggtttgca taccatcacg ctgtctgaat tacacgatgc
      121 gctggacgac ggcaaactca gcatggatac cattgtggcc aacccgctgg tggctaccaa
      181 gcaggaattc gatgccggat tctttgcacc actgaaggat acctgggtgg cgaatatggt
      241 gtaagggtct gttactcctt caccaccagc atactccact gctctccgtc agtctgcaac
      301 ctgaccaggt aaagtccgct tgctacttcc tctcccgatc cattttggcc attccagacc
      361 agagaaccat tccatggtgt gctatagacc ggcctgcctt gcaggtctgt aatggtgatc
      421 aggccattgt gtaccgtttc tgaacgcaga tacaaagcgg tgttgaacgg attgggacct
      481 gcagtgaagg gcagtagttc ataatcttcc actgctgccg gacaaggtag gatgatggca
      541 gttccgggag agccatacgg acagcctgca gtccaggcac cgggcacatt cggtgttcca
      601 tagaagtcta acaactccag tgtaaaacca ttgccgtctg cttcatcagc ccagggtgga
      661 tcatcatggt agtagaggcc gtattgcagg ataccctcat tgtcataaat tcgaacggca
      721 tcaccctgac cactgaaacc gaagcccaga tcgccgatca ggttgtcaat ggcaggatag
      781 taggtggtat attgaaagat gtcggtacaa accaccaggt gggcgccggc ttccagaatg
      841 gtgcctgctg gtatatggaa aacattgttg tctttgctgt cgcggatgct ccagtcggat
      901 atatcgatcg caccgatccc gctgttgacc agttctatcc agtcgccgct gtcgtggtag
      961 aagatagagt tgtaattgat ctcactcagc tgcacagaat agttgcagtc gctgtattca
     1021 ctacccggac ttccgcccac acaacctccg aaccagttgg acggatcgtt gagctctgcg
     1081 gtatagtcat ccacttccag gctcatgcca tagccgtcgg cagatttcgg ccagggcaat
     1141 gtgtcgccgt agctgatctg aatgacgggc gcaccgccgc cgttgaagag ccgaacctga
     1201 ccgcctccgt tatccagacc gaattcaaaa tcgcctatca cattggaaac cgtaggatag
     1261 accatgtgga aggtgtcttc ccactgagca atgaccttga attcgcccgg ttggaggatg
     1321 gtgccatcag gaatttcaaa ctcatccagc acatcttcgc tcttgagcag ccatccgctg
     1381 atgtcgatct gagttgtgcc gtaattgtaa agttctaccc aatcctgacc attcagagag
     1441 ctgtcaggat tgtacatgat ctcggtgatg gcaagatcat aagactgacc gaacagtcga
     1501 agaccggcca gcataaaggc gaagaggagt ccctttttca tacgtgcctt aaaggtaggg
     1561 tatgttatgc gaatgttaag tcaccatcag gcaacaaaaa aggccggtat gaaccggcct
     1621 gttgaattac ttggcgggtg ccggcactac ctgtaccagt tccagatcga ataccagaac
     1681 agagttggca gggatccttc ctccggttct ggagccgtaa ccctgttcag aaggcacata
     1741 gaccatccag tggtctcctt cgtgcatcat ctgaagtatt tcattgaatc cctgaatgac
     1801 ctgccccagg gtcagggtgg caggagcccc cttggatttg ttgctgtcaa actcttctcc
     1861 gttgatgaaa gtgccggtgt agttgaccag caccttatct cccatgacag gactgggagc
     1921 attggcatcg ccgcttttaa ggatcttata ttgtactcct gacggggtgg tcatcacacc
     1981 ctctttcttc gcattttcgg ccaggaattg tttgccttct tccatggttg cattggctgc
     2041 ttccatttgt ttgcgctgga aataggtatt gaggtagttc atacattcct gctcattcat
     2101 ctgcagtgtg gtatctccgg cgtaggccct ttctgccatg gtcagcatca gttcaaaatt
     2161 gagatcgccg gcaccttgtt tcttcagaga ttcaccgata ttgaccccga gggcgtagct
     2221 taccgaatca ttttctgttt taagttcaac tgattgtgcg gttccgcttt tctgacagga
     2281 agagaaaaca gcaacagcta tggctatgaa caacaaattt ctcatgttgt agaagttttt
     2341 tgtgagctgc aaaggtaatt atctgccagc tatctgacag acaccacata ggttttggct
     2401 gcacggtcgt gcaggcttcg cttgcgttca tccagtatga ccatcagaaa accgacacca
     2461 agaatcaggg aggagacgat cttcatcagg ttgcgaatga ccgactgacc caggctgatg
     2521 cgatgtccgg attcatctgt gacctgaagc cccagcagct gtttgcctat ggttccgcga
     2581 cgtgcacctg attccagtaa aatgaagtat aacaagccga ataagctgcc aaaccagatg
     2641 atattgtttc cggttgataa gaatgaaacg ccaaacaaca tggatacgaa caggcgcacc
     2701 aaagccaata tccctgcatc gatcagatag gccactgccc gaattccaaa cccggcaaag
     2761 gagtagtcgg gttccatttc ctgttcatca agaacataca tgacactaag atacaacagg
     2821 actatgcaag tccgcgggat ttcagttcca gatacttgtt gatggtgtgg atggtgagat
     2881 ctgacggact ggtgatgatg gtctgaatgc catacttgtt gagttcctgc atgatcagct
     2941 gcttctccag ggtaaatctt tttgcgaagg tggtgaggta aacactttcc gtattacctg
     3001 catcctgatc tccatacctg ttgatctctt cattctcaaa gaaaaccact accagcagat
     3061 ggagtttgtt gagttttcgc aacacggaga gtgcccgctc tatggcgtag taactttcaa
     3121 aattggtata aaggaacaac agacttcgca cgtgcacatg gcgccttacc tggtaatata
     3181 aatgctcaaa actgctttct tccggtattg ccttttcatt gtacagcagc tcaagtatct
     3241 tacgcatctg acctgcatgg cgatcagctc ttaacactgt gtttacttta ctggcaaagg
     3301 tgatgatgcc tgctttgtct tcctttcgca ctgctatgtt agaaatgaca aggcttgtgt
     3361 tgatggcata gtccatcagg ctgagaccat cgaacggtgt cttcatggta cggctcttgt
     3421 ctatcagaca ataaacctgc tgtgcttttt catcttcata atggttgacc atgagtgatg
     3481 cactgcggcc tgtggctttc cagttgagtg atcgcacatc atcgccgggc acatactgtt
     3541 tgatctgatc gaactcatag ctgtgcccga tccgccgcat cttcttcatt ccctgaaaat
     3601 gggcaatgcg ccggatggtg tacaactcaa acttcttcat ctgcatcaca gaaggataca
     3661 ccttcaccat gtcttccagg tctatactga ccttgcgttc tgccagtccg aataaatatg
     3721 aaatgaatat atgaactttg ccaaaatgat aagcccctct ttccaaaggc ctgaggtggt
     3781 aggtcagctc tctggaagtt cttgcaggta acctgtactg cagttccagg tcacgcttct
     3841 gcaattgttg cggcaactca tcaatgatgc gcaggcggag gcctgccgga aagcgtccgg
     3901 tgatgtggag gtgtacttcg ttctcatcgt gcaaactcat gacctgtggc aacctcctgc
     3961 tcacactcag cggctgttta acgatgaaca gcaaagcggc atctaccaga gtgagtgcgg
     4021 caaagaacaa catagccaca atagccacag gatacaggaa gggaatgaag aacgagaccg
     4081 tatatagaat gataagcccc cccagccacc agaaaaagcg gttgcgcagt gtgatatgtt
     4141 gccagaacct tatcaacgtg gtacctctac tttttccagc atttgcctga tgacatcttc
     4201 ggtactgact ccttccagct ctttatccgg ggtgaggatg atgcggtggt tgagcaccgg
     4261 gtaggcaacc cgatgaatat cgtccggaat aacaaagtct cttccctgta gggctgcata
     4321 ggcccttgag gtgcgcatga tggccagtga tgcacgtggc gatgcaccca ggaacagatc
     4381 cggatggttt ctggtgaggt ggatgaggct tgcaatgtac tgcaataact gatcgctgat
     4441 ggtcactgat tccaccacct gacgaagctg tttcagcaca tcaggtgaaa ccaccgatga
     4501 taccgtttcc tgtactctgg tggtcaggtc ttgtttgaat ctttgcagga tctgcagttc
     4561 ttcctgttct gatggataac cgatgcgaat gcggaacagg aaacggtcca gttgcgcttc
     4621 aggaagcttg taggtgcctt cctgctcgat cgggttttgc gtagcaacta ccagaaaagg
     4681 gaaggagagt acataggtgg tgccgtcggc cgttacctgc tgttcttcca tcacttcgaa
     4741 caaagcggcc tgcgtcttgg ctggtgcccg gttgatctca tcgatcagta cgatgttgga
     4801 aaacaccgga cccttgcgga attcaaagat ggatttctgt tgattaaaaa caggcgtacc
     4861 gatcacatct gatggcatga gatcaggtgt gaattgtatc cggctgaaat ctgcccggat
     4921 ggtcttggcc agcatccggg ctgtcagggt tttggctata ccgggaaccc cttcgatcag
     4981 catgtgtccg ccagacagca atccggtcat cagcaactcg ctgaggccct cctgtcccac
     5041 gatcactttg ccgatctctg ccctgacggc atccatgacc tgtctcagtt gttctacctg
     5101 tgaatttgca ttttccatta ttgacatgat ttataaaacg ttcgtgtttt ggtatagaaa
     5161 tcggtcatct ctttttcttt taatgacaac cggtctttga aacttttgta agtatcgaga
     5221 atatctctga tggccgactc aggcacctga cttttcatag acagtttgct gatgaacagc
     5281 tcctggtctt cgcctgcaac cagctgatat ttatccttga caaatgacct ccagaggcgc
     5341 atttgttgtt gcaccagatg gaggtgatcg ccacgcaggt agtgcatgcg acctaccgtc
     5401 atggcaaact ccagtgagcg gttcacgttg ggctctgcca atgggatgac ctgctgttct
     5461 cttctgccac ggaacaacac aaacaagaat gcgcctgcca gcagcatgta ccaggctgaa
     5521 cggagcgatg gctgagacaa aatgaaccgc aacgggctgt tgaattccgg gtcgcccgcc
     5581 tgctgtgagg gagggatctt gctatactca tccagcaata taccgccttg ttccaggtgg
     5641 ctgaacatga tgttgaaaaa agggatcttg ttgctgtcaa ctgcatagat attggtcagc
     5701 aacacaggat tgcagaacag atacaggtgc ccgttgccga tggtcagatc aagcatggag
     5761 gggtagtgcc ggtcatcatg ggctgctacc tgaaggcctc cggtatctgt ttgaaaatac
     5821 ctccaggaat gagattcctt ctggtcacgg acgcggtaac tgaatcgaaa tcggtctttc
     5881 ggcagtccgg ggtaacggaa atacaggtcg aagtatttgt cgtgataact gttcaggatg
     5941 gcagtgccgg tattagtatc ctgataccaa tcggcttcat tgaacggagg aagaacgttg
     6001 ccggcttctg taccgtcatt aaacagctta cccatcagag taacaggaaa ctcattggct
     6061 gctatcatgg catggttccc cttgcttata taagcgatca gggcatcagc catctcgtct
     6121 gtgtacagtg gtttttcgcc aatgaacagg taattgggtg cctgcgtctg ctccagtgct
     6181 gtgatcagat ctttcctgat ccattcgggt ttttcgtcct ggaaatgttc gagcagatcg
     6241 accgtataag acaggccata cggctgatca gaagacgggc gataggtctc ataccagaag
     6301 taattatggc tgttgaaatt ccgcaccatc cagatgacga aacctgttgc aatggtcact
     6361 cctactacga tggctatgac ggtacctctg ttcattcgcc tgcctccatg cgttgtatga
     6421 gtcgctgcag tttgttgctg tgttccaggt acccctgatg atccatcggc cgcagtccgt
     6481 accatatccg gtcaaacaca agtgactctg tgtgcaggtc atcggccaaa ggatgcagcc
     6541 ccagttccct tacataatca tggttcgtct tttcccgctt ccatatgatg cgacccgtgt
     6601 catgcagggc acgtatcacc agcaggtact gtatcctgaa tgccaggcgc cagtcttcat
     6661 tcctgacagc ttcgcgaagc aaacggtcaa ggtcagactc catcgggcgc tgatccaggt
     6721 cttcgatccg gatttcatct cccgcagcaa cccgcctgtt tcctgctatt cggctgcgca
     6781 gcaaataaaa aatgatcact gccagaatgc caatgatgag cgtgtagccc agtatctgca
     6841 gccatccggt agaaatggtt ctgaccggta cctcctgcat ttcaggaact tcggtgtctg
     6901 gtgtggcttc cctgaactgc tctccgtcaa ctgtttcaga ccattgccgg gtatcctgtt
     6961 cacggtactg aaatccgttt tctgcctgtg cagcgaaaag actgaacagg cctgcaagta
     7021 acagtaatat attaaataaa tgcctcatct atctgtgctt tcagggttcc ggctgatacg
     7081 atctctttgc cgctgaagaa catcagcgac agaccgaaaa cgatcaggct aaataaaaga
     7141 tagtaggagg cgagcgtgat catttgcatg atggcagaga tgatgcggcc tttcatgcta
     7201 aagctgtcac tgaacatcca ttgcagcaat tccgaataga aataggagac actggtatcc
     7261 agcagcagca tcagaatgaa caccgtaatg aacagtaccg ctaccaccat caggatgctg
     7321 ccatattgcc tggataatgg tatgaaaggt tgcaagcgtt caccagttcg cttcagttgc
     7381 catgcacaga tgaggtacac cggtattggc agcaacagca tttgcaaaac agtactggcg
     7441 gtaagtggca tgagaaacag cagcgcccag atactgccgg ccagcaatgc aggaagcagc
     7501 aggcgaaggg tgaacagttg tctgaaattt ccttcgcctg atcgtgtgcg aaaaacgagg
     7561 gctacagaca ggtgcatgac aaaggccata gacagtacat gcagacaaaa aaccgtaagg
     7621 ctgttgcccg gactgatcag gtcatgcagc tcacccagat acagttttct gaatgggttt
     7681 aaggccatat aactgtggtc aaagaaaccg gccagggtaa caagtgcagc aaaggagatg
     7741 ctccaggtga gcaaagcctt caggtgtcgg cgaaacaaag agaatgtgcc ggtataaacc
     7801 tctgactcac ggagaacacg gtcaaacttc acctccggca gaatgaccgg cagcaattca
     7861 tcgggccgct gctctgtttg aaatcctttt cttgcacgac gggcgggata gatcacataa
     7921 tacgtgatca tgaatgcaaa ctctgcaaat atgatcagtg cccgaatcag atccggggta
     7981 tccgtatgcc gggtgaaata agattccaga aatcctgccg tgatgatgat ggggatgatg
     8041 cccatgaaaa ctttcattgc tctgacgcca ccctgcctga atgacgccag acgggaaaag
     8101 gtgccgggaa acagcaggct ccggccgatg atgaatcctg ctgtagcagc aatgacaatg
     8161 gccgacattt ccatggtgcc atggatccag atggtcagaa aagattcacg gaacaaggcc
     8221 cgctctacaa agaaatactg gaaggctcca accatgatgc cattgtacag gagaatatac
     8281 aaggtgccca tgccaaaagt gacgcccagt atgaaactga aaaaggccac cttcaggttg
     8341 ttggtggtaa tggccaggaa catatcattg ggctgcgact ccttatacac tttcatggga
     8401 tcgccgctgc gaatgttctc ttcagtcatg gagatgtaac tgtcgcccag tatgagacgg
     8461 gcaaactcac cgtcatggat actggataac acacctactc caagagccag cagaaagatg
     8521 acagtgctga ataagagctc acggcgactg tggtgcatga cctccggaag ctcgtcagac
     8581 cagaagtgct tgaatttgtt ccagcggtta agcctgttct tgtaaatgct gaaatatacc
     8641 tgctgtgcaa ttctgttcag atagatcctg accgaacgat gcggatagtg ggtgcgggca
     8701 taactcagat catcggtgat ctgcacaaac aggttggtca gtttctccgg gtcaccttgc
     8761 gggctcttga attcttcttc aaattcccgc cactttgctt tattttgatc tatgaaagtg
     8821 gtctccttca ttggatgccc taaaaatata cattgaccct gaatgaacag cattgacatt
     8881 actaccagtc agcaggtggt catcacctat gagctggcca gcgtgagaga ccgcatcctg
     8941 gccttcttcc tggataccat cgtgttgctt ggtctttacc tgggcatctt ctttatattc
     9001 ggcgtgatag gcggcatgca ggattccaat ctgttctact gggtagcgtt tctggtcatg
     9061 tctttctaca gccttttctg ggaattgctg ctcaatggtc agtcgcccgg aaaaagatct
     9121 ctcggcattc aggtcattca tgtgaacgga ctggaaccat cgctgaacag ctacctgctg
     9181 cgctgggcct tcagaaccat agacatctgg ttcaccctgg ggtctgtggc tgtgatgagt
     9241 attagtgcag gagcccgcgg gcaaagactg ggagatatcg tggccaacac aaccgtagtg
     9301 cgcaccgcca acagacagga tgtgcgcatg gaacaattgg aacaattgaa cagcagatca
     9361 aattatgaac caaaatatac cggagttcat gccttcaggg aggagcagat gcttctggta
     9421 aaggaagtgc tggatcgtta tttaaagtat cgtaatacag cgcatcggga agcactggaa
     9481 atgacagctg ctaaagtggc agagcaactg gggctggaaa aaatacctga tgatcttgtg
     9541 gaatttctca aacgggtcat caaagattat gtagctgttt ccagataaat agggtgaaaa
     9601 caccctgttc ctgttcaaat agctccggta cctttgtgcc gggttctctg ttattcccat
     9661 gaagagatca cctgtgaaaa ccaacggggc aggacctgag gagagtcttg cccttttttt
     9721 attctgtaat gctacctttg gatgttgtct gtgtgaaggt attccgtcag acttcccctt
     9781 tttaagcatg cgcagtagaa tatttctcct cattccactg gtgtgcctgc tgcattattc
     9841 cctgcaggca caggtggcag ccagcatcac caaccgagaa tacacccggt tggagatact
     9901 ggaagatctg gactttctgg agcagcacct cactgaaaaa catcccggtc tgtatgaata
     9961 cactgacagt ggctttgtgg tacggtggat ggattcactt cgtcaggccc tgccggaaaa
    10021 actgtctgag caacaactgt atgcagacat cagcagactt tgtcatctga taggcgacgg
    10081 gcataccatg ctcttcccct ccagtaccac cacggcctac tacaatgcat atgaactgtt
    10141 ctttcccatg cgcaccttct gggatggaga acacctttat acctatatgc attattctga
    10201 tgcagatacc attgctgatg gcacaaggat actgtacatc aatgggatcc ctgctgaaga
    10261 agtggtacaa ttctgcctgg atcgcattac gcacgacggt tacaataaga cctacccggt
    10321 gtgggtactg aacaactggt ttgctgagtt ctacggatac ttttacggac atccgtcttc
    10381 ccatacggtg gttat
//
LOCUS       DAOMAX010000070        10322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_874_length_10322_cov_4.410052, whole genome shotgun sequence.
ACCESSION   DAOMAX010000070 DAOMAX010000000
VERSION     DAOMAX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_10322_cov_4.410052"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PK742_05445"
     CDS             complement(<1..757)
                     /locus_tag="PK742_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HQU76068.1"
                     /translation="MQQSVSISNALISVFYKDGLDEIVHELASAGVSLYSTGGTATYI
                     EQLGLPVNQVEELTSYPSILGGRVKTLHPKVFGGILARRDHAGDVAQMEEYALPSFDL
                     VIVDLYPFEETLRSTDDEQAIIEKIDIGGVSLIRAAAKNFRDVAIVSSKDQYSRVLEM
                     LRSQHCSTNLAQRQQLAAEAFDVTRRYDTAIASWMQGGGQNMLSSFGQNNDLRYGENP
                     HQNAAFYGNLNEAFHKIQGKEISYNNLVDIESAV"
     gene            complement(834..3302)
                     /locus_tag="PK742_05450"
     CDS             complement(834..3302)
                     /locus_tag="PK742_05450"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HQU76069.1"
                     /translation="MGAYYTVGQIAGILGAGGQIVSPEAGVGRLFTDSRKLYAGDQGL
                     FIAIRGPHHDGHRYLDEAYRSGIRNFLVSDGGGWQPDANYLLAADTVRALQMLAAFHR
                     QQFAYPVIGITGSNGKTVVKEWIYHLLKTQEDIIRNPRSFNSQTGVPLSVWHMDHHHT
                     LAVFEAGVSRPGEMDHLAEVIRPDIGVFTHFGPAHAAGFLSEEEKAEEKAKLFSQAGV
                     VIYRADIKPVRDACCKLAGEQVSWSMDPEVRALWQVTAEAASLHTTMISLTAENDQLR
                     FTVPFTDEASIENAVCAVLTCLQLHADREQLLGQLDTLPVVTMRLELVAGNNGCQLIN
                     DAYSADAGSLEAALHFLSRQADETPGQEKIVVLSDMEGSGLAEEQLCRQLAVSLQAAG
                     TDRLFAVGPVLSRHAGVFHPIQVHCFPDTGSLLEQVPAAAFHNAIILVKGARRFRLEK
                     VIEAWASKQHNTVLEIDLQHMAHNLHAYRSLLPEQVRVMAMVKAFGYGSGAAEVAHFL
                     AFNRVDYLAVAYGDEGLELRRHGVHLPVMVMNPDPQKVGALIDAELEPEVFSLPQLQA
                     VIQAAADREVRIHIELDTGMNRLGFREEEFADMIRLIRASQRVNPVSIFTHLSAADMP
                     EEDAWSRQQIALFDRWSDSLMQALDRPVLRHVLNSPGIARFPEAAYDMVRLGIGLYGD
                     DPSEELQRKLLPVSSWFTTVAQVRKVEAGESIGYGRQYRAKEPMVLAVLNIGYADGFR
                     RSLGNGVGRVWLHGDYAPVVGRVCMDMCMVDVTHLPDVRPGDKAEVMGTHQTLRQLAE
                     AMKTIPYEVLTGISHRVKRIYQEE"
     gene            3451..6750
                     /gene="secA"
                     /locus_tag="PK742_05455"
     CDS             3451..6750
                     /gene="secA"
                     /locus_tag="PK742_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQU76070.1"
                     /translation="MFTKSLSKLFGGNKYEKDIRAIEPLVAQTNEHFARLSSLTNDQL
                     RGKTNEFRQRIADHLSDIDKEINSIRQEAESLDVEMVDEKDELYRRVDELIKDRDKSL
                     EVILLELLPEAFAVVKETARRFSEHTEVRSTATDLDRDLSVNKDFVVIEGDTAIYHNE
                     WTAAGAPIKWNMVHYDVQIIGGIVLHQGKISEMATGEGKTLVATLPAYLNALAGQGIH
                     IVTVNTYLAQRDSEWNAPIFNWLGITVDCIDKYQPHSPERRNAYNCDITYGTNNEFGF
                     DYLRDNMVRNVEEKVQRKHHYAMVDEVDSVLVDDARTPLIISGPIPKGDQQEFGALKP
                     RITKLVDTQKRIANIFLNDARKLIGEGKTKEGGIALLKAHRAFPKSGALIKFLSEQGM
                     RQLLLDTEGVYLAENNKRMPEIDKDLYFVIDEKSNSVDLTDMGIELITESGEDPGFFV
                     IPDVGAMIADIEKKNLNPDDKVAAKDQLMQDFAIKSERIHSVQQLLKAYALFEKDVEY
                     VVMDGKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYFRMY
                     HKLAGMTGTAETEAGELWEIYKLDVTVIPTNKPIVRDDMNDKVFKTRKDKYNAVIARI
                     EELVGQQRPVLVGTTSVEVSELLSRMLKLKGVKHNVLNAKQHQREAEVVAEAGLAGAV
                     TIATNMAGRGTDIKLGPGVKEAGGLAILGTERHDSRRVDRQLRGRAGRQGDPGSSEFF
                     VSLEDNLMRLFMSDRIAKVMDTLGYKEGDVIEHSSVTKSIERAQKKVEENNFGIRKRL
                     LEYDDVMNSQRELIYRKRNHALAGERLKLDLNNTFHDLCDEISGNWTGTGDYEGFTLD
                     VIRYLGMEPEVSKDKFSDARQEELTGMLYQTAMQFYKRKQQAMVQKVLPLIKDLHANR
                     GETLENVVVPFTDGRKGLNIVVNLQKAVETDGREVALAIERTVSLMIIDDHWKDHLRN
                     MDDLKQAVQGAVYEQKDPLLIYKFEAFKMFREMLSELNMEIAGFLFKGNVPLRSPEEV
                     QQAAAQRRTDMSQMRTSRQEEVAERGPGNRQPEKRNIEPVRVEKKVGRNEPCPCGSGK
                     KYKNCHGKDEA"
     gene            6763..7434
                     /gene="deoC"
                     /locus_tag="PK742_05460"
     CDS             6763..7434
                     /gene="deoC"
                     /locus_tag="PK742_05460"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQU76071.1"
                     /translation="MSDTILRLRTAIEHTILKPDTIPADIQRICREAAEYGFAAVCVP
                     PYYVTDAAELLQESAVELATVIGFPYGYSSLMAKFEEAEDALAQGADHLDVVINIAAV
                     KAGDWDTINNEMDMLTRLVHTEGKVIKYIAETGLMTEIETDLICRMANDHGIDYLKTS
                     TGVNGPGASVEQVTSLRKLLRADIRIKASGGIRTREDAIALLDAGADRLGTSSGVALM
                     ADNAS"
     gene            7454..7843
                     /locus_tag="PK742_05465"
     CDS             7454..7843
                     /locus_tag="PK742_05465"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQU76072.1"
                     /translation="MRYLFFTICTIGMLSARAQQGSVVIHDHAGAESLVEKHQYFNEE
                     HNQIPGWRIQVISTPSMTEAKGEKTRVLQQFDNQAFIVFEAPNYKVRLGNYRNRFDAY
                     RDLQDVLSMYPNAFICKDLINVREPQP"
     gene            7840..9030
                     /locus_tag="PK742_05470"
     CDS             7840..9030
                     /locus_tag="PK742_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HQU76073.1"
                     /translation="MSPERIKELSRQYFEHVRTLRRHLHSHPELSFQEYETAAFVAEE
                     LRNMGLQPHTGIAGTGVTAIIEGQAGSAVIALRADMDALPIQEENEVEYRSQHDGVMH
                     ACGHDVHTSSLLGAARILQETKDNWRGSIRLIFQPGEEKLPGGASTMIAEGVLKNPSV
                     DGIFGQHAFTPYKVGTVAFCPGRMMASTDELYLTVRGKGGHAAYPHLLKDPVPVAARI
                     IDALHQITGRLISPLEPAVVSIGKVEARGATNVIPDEVHMEGTVRAMNEDIRNTILEA
                     VERITRQTAAASGMEVVVDIRRGYPSLVNDVEMTGRAIARAENYLGKEQVFLAEPRMG
                     AEDFAFYSQEIPACFYRLGTGNPEKGITSFIHTPTFDIDEDALMTGMGLMAWNAIEEL
                     QFRS"
     gene            complement(9015..9791)
                     /gene="truA"
                     /locus_tag="PK742_05475"
     CDS             complement(9015..9791)
                     /gene="truA"
                     /locus_tag="PK742_05475"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQU76074.1"
                     /translation="MSSYLCVVRYFLEIAYDGTAYNGWQVQPNGVTVQETVDKALSTI
                     LRDAIHCIGCGRTDAGVHASQFYLHFDTDMPVPDDFLQRMNAFLPKDIGVYRLLPVHD
                     KAHSRFDARSRSYEYVLCRQKDVFRFGYVYYKPFHKLDLDRMQQACVILQQFHDFPSF
                     CKSKAGSKTTLVRIDEAKWEERGNDLVFCITADRFLRGMVRLVVGAMLQIGEGKMSLE
                     EFEKGIREKQRFRLALSAPAHGLYLNHVRYDYIPERQDLN"
     gene            9808..10203
                     /locus_tag="PK742_05480"
     CDS             9808..10203
                     /locus_tag="PK742_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HQU76075.1"
                     /translation="MWQRLQTLWMALAIICMLIYLLLPTGHAGDLGFSTKDDILRAAL
                     AVAVMALSLFAITSYRNRKLQMRINLISVALIAAIYVLWLISEERSQEAYTVAWPMFL
                     PGFAILLLLLANRGIGRDERLVKSMDRLR"
BASE COUNT         2685 a         2803 c         2559 g         2275 t
ORIGIN      
        1 aaacggcaga ttctatatct actaaattat tataagatat ttccttaccc tgtatcttat
       61 ggaatgcttc attcaggttg ccgtaaaagg ctgcattctg atgtgggttc tcgccatacc
      121 gaaggtcgtt attctgacca aagctgctca gcatgttctg cccaccgccc tgcatccagg
      181 aggcaatagc agtatcatac cggcgggtca catcaaatgc ttcggctgcc agttgctggc
      241 gctgcgcaag gttggtgctg cagtgctggc tgcgcaacat ctccagtacc ctgctatatt
      301 gatctttgga agaaactatg gccacatccc ggaaattctt ggccgcagca cgtatcagtg
      361 atacgccgcc aatgtctatc ttctctatga tggcctgttc atcatctgtg ctgcgcaagg
      421 tttcctcaaa aggatacagg tcaacaatga ccagatcaaa tgagggcaaa gcatactctt
      481 ccatctgcgc tacatcgccg gcatgatccc ttcgggccag aatgccgcca aacacttttg
      541 ggtgtagtgt tttcaccctt ccacccagaa tgctggggta agaggtcagc tcttccacct
      601 ggttaacagg taaaccgagt tgttcaatat aggttgcagt gccgcctgta gaatacagcg
      661 aaacacctgc tgatgccagc tcatgaacga tctcatccag accgtcctta tagaaaaccg
      721 agatcaatgc attggaaatg gaaacagatt gttgcatgcc tgaaaaagat gtgcaaagtt
      781 agcgcaatca aggcataaaa ggaacgggaa gcctagcaat tattgcgttc aggtcactct
      841 tcctgataga ttcttttcac acggtgcgag ataccggtaa gcacttcata gggaatggtc
      901 ttcatggctt ctgcaagttg cctgagggtc tggtgtgttc ccatcacttc tgctttgtcg
      961 ccgggacgaa catccggcag atgggtcacg tcaaccatac acatgtccat acaaaccctg
     1021 cccaccaccg gcgcataatc gccgtgcagc cataccctgc caactccgtt tcccaggctt
     1081 cgcctgaaac catcggcata gccaatattc aacaccgcca gtaccatagg ttcttttgcc
     1141 cggtattgtc tgccataccc gatgctctct ccggcttcca ctttccttac ctgcgccact
     1201 gtggtaaacc agctgcttac gggcaacagc tttctctgca gttcttctga gggatcatcg
     1261 ccatacaaac cgattccaag cctgaccatg tcataggctg cttccggaaa cctggcaata
     1321 cccggagagt tgagcacatg ccgcagtacc ggtcgatcca aagcctgcat caggctgtca
     1381 ctccatctgt caaaaagggc tatctgctgc ctggaccagg catcctcttc aggcatatca
     1441 gcagcactga gatgggtgaa tatactgacc ggatttaccc gttgcgatgc ccgtataagc
     1501 cggatcatgt cagcaaactc ttcttcccgg aaacccagcc tgttcatacc ggtatcaagt
     1561 tcaatatgga tgcgcacctc acgatctgcg gcagcctgaa tgaccgcctg caactgaggc
     1621 aggctgaaca cctccggttc cagttcagca tctatcagcg cacccacttt ttgcggatcg
     1681 ggattcatga ccatgaccgg aagatgcaca ccatgtctgc gcagctccag tccttcatcg
     1741 ccataggcca ctgccagata gtccacccgg ttgaatgcca ggaaatgcgc cacttctgca
     1801 gcgccgcttc cataaccgaa agccttgacc atggccatta cccgaacctg ctccggcagc
     1861 agtgaacggt aggcatgcag gttatgcgcc atatgctgca gatcgatctc cagcactgta
     1921 ttgtgttgtt tcgatgccca ggcttcaatg accttttcca gccggaaacg cctggcgcct
     1981 tttaccagga tgatggcatt gtgaaaagca gctgccggca cctgctccag caaactaccg
     2041 gtatccggaa aacaatgtac ctgtatggga tgaaagactc ctgcatgcct gctcaaaacc
     2101 ggaccaacgg caaacaatct gtctgtacct gctgcctgca aactcacagc gagctgcctg
     2161 cacaactgct cctctgccag gccactgccc tccatatcgc tcagcaccac gatcttctcc
     2221 tgacctggtg tttcatctgc ctgcctggat aaaaaatgca gggctgcttc cagtgatcct
     2281 gcatcagcac tgtaggcatc attgatcagc tgacaaccat tgttgcctgc taccagttca
     2341 agcctcatgg tcactaccgg caaagtgtcc agctgaccaa gcagctgttc ccggtctgca
     2401 tgcagctgca agcaggtcag cactgcacaa acggcatttt caatagaagc ttcatctgtg
     2461 aacggaacgg tgaagcgcag ctggtcattc tctgcggtca ggctgatcat ggttgtatgc
     2521 aaggaagcgg cttctgccgt tacctgccac aacgctctga cctccggatc catgctccag
     2581 cttacctgtt ctccggccag cttgcagcag gcatcccgca caggtttgat atctgcccgg
     2641 taaatgacca ctcctgcctg actgaacaac ttagctttct cttctgcctt ttcctcttct
     2701 gataaaaaac ctgctgcatg cgccggtccg aaatgggtga aaacacctat gtctggtctg
     2761 atgacctctg ccagatgatc catctctccg ggccgggaaa cacccgcctc gaacactgcc
     2821 agtgtatggt gatgatccat atgccagact gataagggta cccctgtctg tgagttaaaa
     2881 cttcttgggt tgcggatgat gtcttcctgt gtcttgagca gatgatagat ccactctttg
     2941 accacggtct tgccattgct gccggtgatg cctatgaccg gataagcaaa ctgctgccgg
     3001 tgaaaggctg ccagcatctg caatgccctg accgtatcgg ctgccagcag atagttggcg
     3061 tctggttgcc agcctcctcc gtcagacacc agaaagttgc gaataccact gcgataagcc
     3121 tcatccagat acctgtgccc gtcatgatgc ggccccctga tagctatgaa cagaccctga
     3181 tcgccggcat aaagctttct ggaatctgtg aacaagcggc ctactcccgc ctccgggctg
     3241 acaatctgtc cgcctgctcc caatattcca gcgatctgtc cgactgtata ataagccccc
     3301 atgaatgacg taaaagtacg gtatggaaaa ggaatcacgc accaggccgc cggacgctaa
     3361 taattgtatc tttgtgaccc tgcagaactg tcaggaccat ttaccgggga acggtttttg
     3421 ccccggaaca tactttcgca gcttatgaat atgtttacca agtccctgtc gaagctcttc
     3481 ggtgggaata aatacgaaaa agatatcaga gccatagaac ctctggtagc tcaaaccaac
     3541 gaacattttg cacgtctgtc atcccttacc aacgaccagc ttcgcggtaa gaccaatgag
     3601 ttcagacaac gcatcgctga ccacctgtct gatattgata aggagatcaa cagcatccgt
     3661 caggaagctg aatcactgga cgtggaaatg gtggacgaga aagatgaact ctatcgccgt
     3721 gttgatgagc tgatcaaaga ccgcgacaaa tctcttgagg tcattctgct cgaactgctg
     3781 ccggaagcct ttgctgtggt caaggaaact gcaagacgtt tcagtgaaca caccgaagtg
     3841 cgaagcaccg ctactgacct ggaccgcgac ctgtctgtca acaaagactt cgtggtcata
     3901 gaaggagata ctgccatata ccacaacgaa tggaccgctg caggtgcgcc tatcaaatgg
     3961 aatatggtgc attatgatgt acagatcatt ggtggtatcg tattgcacca gggcaagatc
     4021 tcagaaatgg ccacaggaga aggcaagacc ctggtggcaa ccctgcccgc ctacctgaat
     4081 gcactggcag gacagggtat tcacattgtt acggtcaaca cctatctggc acagcgagac
     4141 agcgaatgga atgccccgat attcaactgg ctgggcataa ccgttgattg tatagacaag
     4201 taccagccgc actctcctga acgaagaaat gcttacaact gcgatatcac ctacggtacc
     4261 aacaatgagt tcggttttga ctacctgaga gacaacatgg tgcgcaatgt tgaagaaaaa
     4321 gtacagcgca aacaccatta tgccatggta gatgaggtgg actctgtact cgtggatgac
     4381 gcccgtacac cgctcatcat ttccggacct atcccgaaag gcgatcagca ggaatttggc
     4441 gccctgaaac cacgcatcac caaactggta gatacgcaga agcgcattgc caatatcttc
     4501 ctgaatgatg ccagaaaact gataggcgaa ggcaagacga aagaaggcgg tatcgccctg
     4561 ctgaaagccc accgtgcttt cccgaaaagt ggtgcgttga tcaaattcct gagtgaacag
     4621 ggaatgcgcc agctgctgct ggacaccgaa ggggtgtacc tggcagaaaa caacaagcgc
     4681 atgcctgaga tcgacaagga cctctacttt gtgatcgatg aaaaatccaa ttcggttgac
     4741 ctgaccgata tgggtataga actcattacc gaatcaggcg aagacccggg cttcttcgtt
     4801 attccggatg tgggcgccat gatagccgac attgaaaaaa agaacctcaa ccccgatgat
     4861 aaggtggctg ccaaagacca gctgatgcag gactttgcga tcaagagcga acgcatccac
     4921 tctgtacaac aactgctcaa ggcatatgcc ctgtttgaaa aagacgtgga gtatgtggtg
     4981 atggacggaa aggtcaagat cgttgatgag cagaccggac gtatcctgga aggccgccgc
     5041 tacagcgatg gtctccacca ggccatcgaa gccaaagaga atgtgaaggt ggaagctgcc
     5101 acgcaaacct atgccaccat caccctccag aattatttcc gcatgtacca caagctggcc
     5161 ggtatgaccg gtaccgccga aactgaagcc ggcgaattgt gggagatcta caaactggac
     5221 gtgaccgtta tcccgaccaa caaacctatt gtgcgtgatg acatgaacga taaggtcttc
     5281 aagaccagaa aagataaata caacgccgtt attgcccgca tcgaagaact tgtagggcag
     5341 caaagaccgg tgctggttgg tactacctct gtggaagtat ctgagttgct gagccgcatg
     5401 ctcaaactca agggtgtcaa acacaatgta ctcaacgcca aacaacacca gcgggaagcc
     5461 gaagtggtgg ccgaagccgg tctggccggg gcagttacca ttgctaccaa catggctggt
     5521 cgtggtaccg acatcaaact cgggccgggc gtgaaagaag ccggtggtct ggccattctc
     5581 ggtactgaac gccacgactc acgccgggtg gacagacagc tgcgtggacg ggccggccgg
     5641 cagggtgacc ccggttcttc tgaattcttc gtgtctctgg aagacaacct gatgcgcctc
     5701 ttcatgagcg accgtattgc taaagtgatg gacaccctgg ggtacaagga aggtgatgtc
     5761 attgaacaca gcagcgttac caaatctatt gaaagagcgc agaaaaaagt ggaagaaaac
     5821 aacttcggca ttcgaaagcg cctccttgag tacgatgatg tcatgaacag ccagcgtgaa
     5881 ctcatctacc gcaaacgcaa ccatgcactg gccggcgaac gcctgaaact ggacctcaac
     5941 aacaccttcc acgacctgtg tgatgagata tcaggaaact ggaccggcac cggcgattat
     6001 gaaggattca ccctcgatgt gatccgctac ctcggtatgg aaccggaagt gagtaaggat
     6061 aaattctctg acgcccgcca ggaagaactg accggtatgc tctaccagac tgctatgcag
     6121 ttctacaagc gcaagcaaca ggccatggtg cagaaagtgc ttccgctcat caaagacctg
     6181 catgccaaca gaggcgaaac cctggaaaat gtggtggtgc catttactga cggccgcaag
     6241 ggtctgaaca tcgtggtcaa cctgcaaaag gctgttgaaa ctgatggtcg cgaagtagcc
     6301 ctcgccattg aacgcacggt gagtctcatg atcattgacg accactggaa agatcacctg
     6361 cgcaacatgg acgacctgaa acaggccgtg cagggagctg tgtacgaaca gaaagacccg
     6421 ctgctcatct ataagttcga agcattcaag atgttccgcg aaatgctgag tgaactgaac
     6481 atggaaattg ccggattcct ctttaaagga aatgtgccgc tgcgttcgcc ggaagaagtt
     6541 cagcaggccg ctgcccaacg tcgcaccgac atgagccaga tgcgcaccag cagacaggaa
     6601 gaagtggctg aacgaggccc cggaaaccgc cagcccgaaa aacgcaacat tgaaccggtg
     6661 cgggtagaga aaaaagtagg tcgcaatgag ccctgcccct gcggaagcgg gaaaaaatac
     6721 aagaactgtc acggcaaaga cgaagcctga taatacagct acatgtctga taccatcctg
     6781 cggctgagaa ccgccataga acataccatc ctgaaaccgg ataccattcc ggccgatata
     6841 caacgcatat gcagggaagc cgcagaatac ggtttcgcag ccgtttgtgt gccaccctat
     6901 tatgtaacgg atgccgccga actgctgcag gaaagcgccg tagaactggc caccgtgatc
     6961 ggatttccct atggctacag cagcctgatg gccaaatttg aagaagccga agacgccctg
     7021 gcacaaggcg cagaccacct ggatgtggtc atcaatattg ctgctgtcaa ggccggcgac
     7081 tgggatacca tcaacaacga aatggacatg ctgacccgcc tggtacatac agaaggtaag
     7141 gtcatcaagt acattgcaga gaccggattg atgacagaga tcgagaccga tctgatctgt
     7201 cgcatggcca acgaccacgg gattgattac ctcaagacct ctaccggcgt gaacggtccc
     7261 ggtgccagtg tggagcaggt taccagcctc agaaaacttc tgcgggcaga catccgtatc
     7321 aaagcaagcg gaggtatccg cacccgcgaa gatgccattg ccctgctgga tgcaggagca
     7381 gacagactgg gcacctcttc cggcgttgca ctgatggctg acaatgcctc ctgatattta
     7441 ctaactttga actatgcggt acctgttttt caccatctgc accataggta tgctcagcgc
     7501 ccgggcacag caaggcagcg tggtcattca tgaccatgca ggagcagaaa gcctggtgga
     7561 aaaacaccag tatttcaatg aagaacacaa tcagatacca ggctggcgca tccaggtgat
     7621 ctctactccc agtatgaccg aagccaaagg tgaaaagacc agggtgttgc agcagtttga
     7681 caaccaggca ttcatcgttt ttgaagcacc caactacaag gtcagactgg gcaactaccg
     7741 caatcgcttt gacgcttacc gcgacctgca ggatgtgctg tccatgtacc ccaatgcctt
     7801 catctgcaag gacctgatca atgtgcgtga accccagcca tgagccccga acgcattaag
     7861 gaattatcca gacagtattt tgaacacgtg cgcaccctgc gcaggcacct gcacagccat
     7921 ccggagctat cgttccagga atatgagacc gccgcatttg ttgctgaaga actcaggaat
     7981 atgggccttc aaccgcatac aggaattgcc ggaacgggcg taactgctat tatagaaggt
     8041 caggcaggca gtgctgtgat agcgctccgg gcagatatgg atgccctgcc catccaggaa
     8101 gagaatgagg tggaataccg gtctcaacac gacggtgtca tgcatgcctg cggacacgac
     8161 gtacatacca gttcactgct gggtgctgca cgcattttgc aggaaacaaa agacaactgg
     8221 cggggaagca ttcgcctgat ctttcaacca ggtgaagaaa aactgcctgg cggcgccagt
     8281 accatgattg ccgaaggtgt gttgaagaat ccttcggtcg atggcatttt cggacaacac
     8341 gcatttacac cttataaagt gggaaccgtg gctttctgtc ccggacgcat gatggccagc
     8401 acagatgaac tgtacctgac cgttcgcgga aaaggcgggc atgccgctta tccccacctg
     8461 ttgaaagatc ctgtgcctgt ggctgcccgt atcattgatg cgctgcacca gatcaccggc
     8521 agactgatat cgccgctgga acctgccgta gtgtccatag ggaaagtgga agccagaggg
     8581 gctaccaatg tcatccccga tgaggtacac atggagggca ccgtgcgggc catgaatgaa
     8641 gacattcgca ataccatact ggaagcggtg gaacgcatca cccgacagac tgctgccgct
     8701 tcaggtatgg aagtggtggt cgatatacgc cgcggatacc cttctctggt caacgatgtg
     8761 gagatgaccg gaagagccat cgccagagca gaaaactacc ttggaaaaga acaggtattc
     8821 ctggcagaac cacgcatggg tgccgaagat tttgccttct attctcagga aattccggct
     8881 tgtttctacc ggctgggaac aggcaatccg gaaaaaggga tcacctcttt catccataca
     8941 cccacctttg atattgatga agatgcactg atgaccggaa tgggattgat ggcctggaat
     9001 gccatagaag aacttcagtt cagatcctga cgttccggaa tatagtcgta ccgtacatga
     9061 ttcagataaa gtccatgagc gggcgctgat aatgccagcc ggaatcgctg cttctctctg
     9121 atgccttttt caaattcttc cagactcatt ttaccttccc ctatctgcag catcgcaccc
     9181 accacgagcc gcaccatgcc acgcaaaaaa cgatcggcgg taatacagaa taccaggtcg
     9241 tttccccgct cttcccattt cgcttcatct atccgcacca gcgtggtctt actgccggcc
     9301 ttgctcttgc aaaaactggg aaagtcatgg aactgctgca ggatgacaca ggcctgttgc
     9361 atcctgtcca gatccagttt gtggaatggt ttgtaataga cataaccaaa acgaaataca
     9421 tccttctgcc tgcagagaac gtactcatat gacctggaac gtgcatcaaa acggctgtgt
     9481 gctttatcat gtaccggcaa taaccggtac accccgatat ccttcggcag aaaagcgttc
     9541 atgcgctgta aaaagtcatc gggaacaggc atatctgtat caaagtgcag atagaactga
     9601 gatgcatgta caccggcatc tgtcctgccg cacccgatac aatgaatagc atcacgtaga
     9661 atggtactca gtgccttgtc aaccgtttcc tgtacagtaa ccccgttggg ctgcacctgc
     9721 catccattgt atgccgtgcc gtcataggcg atctccagaa aatagcgcac cacacaaagg
     9781 tagctgctca caccttatat ttgccgcatg tggcaacgac tgcaaaccct gtggatggcc
     9841 ctggccatca tctgtatgtt gatctatctg ctgcttccaa ccggacatgc aggagatctt
     9901 ggtttctcta ccaaagacga tattctgagg gctgctctgg ctgtagcggt catggctttg
     9961 tcattgtttg ccatcacttc ctatcgtaac agaaaactgc aaatgcgcat caacctcatc
    10021 agtgtggcgc tgatcgcagc gatctatgta ctttggctga tcagcgaaga acgcagccag
    10081 gaggcatata cggtggcctg gcccatgttt ctgccgggct ttgccatcct cctcctgctg
    10141 ctggccaaca ggggcatagg ccgcgatgaa aggctcgtga agagcatgga cagactgcgt
    10201 taaaatatcc ataaatacag gcatttaccc acatcttaat atgcaattaa agcgtaatgc
    10261 tttactttgc agtgaacctg gtttatgcgt acctccaact ggcttttggg cctcattatt
    10321 tt
//
LOCUS       DAOMAX010000071        10297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_879_length_10297_cov_3.989260, whole genome shotgun sequence.
ACCESSION   DAOMAX010000071 DAOMAX010000000
VERSION     DAOMAX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_879_length_10297_cov_3.989260"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="PK742_05485"
     CDS             <1..1823
                     /locus_tag="PK742_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HQU76076.1"
                     /translation="AGRAIALFFVLFTPGCSGLKAQEVLVPEWAKSAIWYQIFPERFR
                     NGDPSNDPTLQDIMGCWPHDSITPWQLSPWTSDWYEKQPWEQEAPYDMGGIITRRRYG
                     GDIQGMLDELAYIKSLGVNAIYLNPVFWAPSLHKYDAIMYHHIDPHFGPDPEGDKAII
                     ATEDPLDPRTWKWTSADRLMLEFIKACHQRHIRVIFDGVFNHMGIRSFAFQDVLKNQQ
                     NSPYKDWFTIYSWDTDTSAFSYQGWFGVPDLPEIREDEQGIVNGPKSYIYNCTSRWMM
                     PNGNVANGIDGWRLDVAFCVDHAFWKDWRMLVKAINPDAYLTAEVVDKIEVVSPYLEG
                     DEFDAVMNYGFGVVSSEFFINRDRQISVSEFDKRLRELRNAFPADITYVQQNLFDSHD
                     TQRFTSYIVNHDVAYFRDWGDYFNKTRANNPAYDERKPTAEEYRIQKLMALFQFTYVG
                     APMVYYGDEVGMWGANDPDCRKPMVWDDLAYNDEVYNADGSTRTPDKVSPNMEMHDWY
                     ANIARLRGAYKVFSLGSFETLLTDDETGVYVYRRRLEGEPDAVVILNNSEQDQQVDIS
                     QWLKGTVYNPMKKVEQYFPSKDPMVTVPYKSGLVLMSYTK"
     gene            2378..4057
                     /locus_tag="PK742_05490"
     CDS             2378..4057
                     /locus_tag="PK742_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76077.1"
                     /translation="MTSIIQQLGFSQLQISSLISLLSVTFCVNGQVAFGPVIESSECL
                     SSPGNDAFVDAIKSKDGGILVLGYLRESVEGIIIPEGYLAPTVVIKKNADLENQWIQF
                     IGGSDLGTSGHTILEDEDGSIYCIGTSGANTGDILGNNGVKDIFITKLTSEGDKLWTT
                     VIGSTATDEVTSAGFTSTGDILVTGYSFGDGGDIPYHYASFTSTDVIYFSIDTSGNLN
                     WLKVFGGTGVDQPVFNTVLNNDGNTLICIASSSADHDLVPYGLTDNWKFIMMEVDADG
                     NTIYSTGMDNDSTLEVFEGAMLLKEDSMLVFTTMSRYADLAVSKGIEDGAIVVFTPEL
                     ELDRMYNYGGSNYDYLLRAIYEPTKQVYYVLGSSYSIDQDLPGNYSGGIGDDFWLMSV
                     DEEFNLLWSRNFGGANDSSSFGDAAWYVPGNMIYLSEKLHLFTSCYPTDNPPDFDLNC
                     TPSTGIFTTTDGWRVTFDLLSMDIISDQGKNDIINIYPNPANDIINISCIDCTNISAI
                     RLTSVYGQDLLMKNSNCNEEFEIDVSGFSRGIYFIIVSVDAANFIYPIILN"
     gene            4206..8828
                     /locus_tag="PK742_05495"
     CDS             4206..8828
                     /locus_tag="PK742_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76078.1"
                     /translation="MGRATALSVDPNNTNDIVLGTRSSGIWKTTDGGSNWINITGFTN
                     PGLGIDRIVRYNTTTDVLLATTQFNGSDVSLYSAGLFKSSDAGGSWSEVSSYPSEYEL
                     TGYPWLTALASSESDDLFYIGNKQKMFSISTGWSWLSKPGYPSEKVFLDFDDISTDAS
                     QNSHTISDIDLKPSDHDFIVVSPRNGTYTDYVYSKDGGETWSSFNIPVSPSIPSGYDG
                     YEMEIKTHIMDDGDSYAILVYKYYSNTSSTTSYEAYIFTYDLDLPTPYSTGTYPLTSM
                     DYKGVVTDFFVIDGDGSTVGDICIFSLSQRNVKRTELPFSPGSATVSAISSYGAEDPG
                     THIDVRSMEVIYNSSSNDYEAFIAHDGGISNCADIENAVGDDDWTNINGNGLHITEFR
                     GFGVSEFENGRILATAIDGNGFLFKRSLTYPYEIEILEPFGGDNYRSEVSNHDRDIGV
                     FAYNGTGTKSDASISSFNLSTVALLNDLSFEIPDGLTTPDKTKWEYNYALRPFDFDVY
                     GSVWAGNLDIYEYEYDPLATHVISTNGDLRTETSIHWLSPPSPATKNPISAYKRVDDS
                     NGSDVITWFATKKERLLGSPYADPVKIVSQAYDRDAQSISPADDITPTFDEDGNSSNG
                     IRNQLDYSFVSCIETNDDDPEGLWVSFAGNTQGGHGQILENVLEDGDTARFGRIYYYD
                     LATEEWENRSTGLPNYPVHCLEYWRGSDDVVFAGTDVGVFVWNASSSQWECFDTGDQL
                     PYYSINKLEINYCTMKLYASAFGGGLWETDLPIGFPGSRPYTNAMVISSDVTWEQDFD
                     ARVDIVVKPGASLTIKNCEVRMPKDHRIVVERGAVLWLDNAVITNYCDQWVGVEVWGN
                     ESKPHPTSASSIIAGTYPSATDDQGVVVMLNGSVISNAKIGIGTYRNNGGVLGPGYNG
                     GIVIAEDGNFLNNTVGVKFMPYDYPETIGGLDYENTDDDNVSYFEYCTFTTNDKIFGD
                     GHQDYQVYMDDVDGVQFYGCTFENEIDETTLSNLGSGIYSADATYYVGAASCGTSCED
                     SSTFRGYFRAIEAANVASLPVLDITIKKSVFEENQRGILLSNVLHSSVIQNRFEVNNA
                     SSMQNYGLYLEDCEDYEVEENLFKGVGTGGESPLSAGIYVANNSNAVTEIYRNTFRYL
                     ESGIRSQTVNSKLQIKCNVFDTDIYVYNIAVTSGGIPNQGYCTGNVADPAGNVFSNDF
                     IYEGDYKAQTGVATFKYRHHTDLDPMYYSSSLITLQDCNIYSTSGDACPTNFPSGGGG
                     EESSSMMMSEAGSMDSEIDAIMSSLAGFGGMELGESEAYDTFMEELAYYQDKKETLLK
                     DAYLSYVMEGKMDSAEQVLLNEAEVWAKTYLISHYINSGKFKSASEVIKSIETSDDQL
                     IDFLNFSQLIIELKSTGQTLTSVTDDNLAQVEALSGKQSPAGVACENLLQSINGIDIP
                     EVFDEITTFEEGRTSQAEAIESMVSIYPNPTDKQLFVSLNGVGEESDVYGIYTISTTN
                     GKLSLSGMLKDQLSTLDVSMLSSGLYIITITLNSNGKIHSEKLVIE"
     gene            complement(8864..9643)
                     /locus_tag="PK742_05500"
     CDS             complement(8864..9643)
                     /locus_tag="PK742_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQU76079.1"
                     /translation="MNVYFIDAFTDHVFGGNTAAVVPLQEWLSDAQMQRIAAQHNLSE
                     TVFFIPRGDAFDIRWFTPVTEVDLCGHATLAASYVIWNMLGHPVSYIIFQSKSGALRV
                     TRNNGDLTLDFPMDYYLDQAAPDKMVQALGKAPKEAYIGKTNHLLIYDSEDDIRRLKP
                     DFQLLSEVKAHGIIVTARGYDVDYVYRFFAPSVGINEDPATGSAQTTLTNYWSKKLER
                     ETWISLQLSSRIGKFQTAIEDDRVLITGQAVVYLTGKINVG"
     gene            complement(9640..10104)
                     /locus_tag="PK742_05505"
     CDS             complement(9640..10104)
                     /locus_tag="PK742_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQU76080.1"
                     /translation="MESARIQHLWEQLYDGDPWTWIRLKDILKDVDAVQAACHPIPNA
                     NSIWQLVQHCSGWRRNVLRKMQGEAFRSPDDNYLSEPDNVSPQAWEQLLADFEQVDTD
                     WRNFISTLSDEDLDRPYAPADGKYTWYAVIHGLMHHDNYHFGQIIMLKKMLP"
BASE COUNT         2978 a         2021 c         2404 g         2894 t
ORIGIN      
        1 ctgctggcag ggctattgcc ctgtttttcg tcttgttcac tccgggatgt tccggcctga
       61 aagcgcagga agtgctggta cctgaatggg caaagagtgc catctggtac cagatatttc
      121 ccgaacgttt ccgcaacgga gatccatcca atgatccaac acttcaagac atcatgggct
      181 gctggccgca cgacagcatc acaccctggc agctgagtcc atggacctcc gactggtacg
      241 aaaaacaacc ctgggagcag gaagcacctt atgatatggg cggcatcatc acccgaagaa
      301 gatatggcgg agatattcag gggatgctgg atgaactggc ttatatcaaa tcacttggtg
      361 tgaatgccat ttatctgaat cctgtgttct gggcaccgtc actgcataag tatgatgcca
      421 tcatgtatca ccacatcgat ccgcacttcg ggcctgatcc ggagggtgat aaggccatca
      481 ttgcaacaga agatccgctg gatccgcgaa cctggaaatg gaccagtgca gacaggctga
      541 tgctggagtt cataaaagct tgtcaccagc ggcatatccg tgtcattttc gacggggtgt
      601 tcaaccatat gggtattcgc agtttcgcct ttcaggatgt gttaaagaac cagcaaaatt
      661 ctccttacaa agactggttc accatctata gctgggatac tgatacctca gccttttcat
      721 accagggatg gttcggtgta ccggatctgc cggaaatcag agaagatgag cagggtatcg
      781 tgaacggtcc gaagtcttat atctataact gcacttccag atggatgatg cccaacggaa
      841 atgtggccaa tggcatagac ggctggcgac tggacgtagc tttctgtgtg gatcatgctt
      901 tctggaaaga ctggcgtatg ctggtgaaag ccatcaaccc cgatgcatat ctcacagccg
      961 aagtagtgga caagatagaa gtggtcagtc cctatctgga gggggatgaa tttgatgcgg
     1021 tcatgaacta tggatttggg gtcgtgtcaa gcgagttctt catcaaccgc gacagacaga
     1081 tatccgtttc cgaattcgac aagcgtttga gagagttgcg caacgctttt cccgccgaca
     1141 ttacctatgt gcagcagaat ctgttcgaca gccacgacac ccagcgtttt accagttaca
     1201 ttgtcaacca cgatgtagcc tacttccgcg actggggtga ttacttcaat aaaacaagag
     1261 ccaataatcc ggcatacgac gaacgcaaac caacagcgga agagtatcgc attcaaaaac
     1321 taatggctct gttccagttc acttatgtgg gggcacccat ggtatattac ggagatgaag
     1381 taggtatgtg gggtgccaat gaccccgatt gccgaaagcc catggtgtgg gatgatcttg
     1441 cgtataatga tgaagtctat aatgccgacg gaagtacccg cacaccagac aaagtatcgc
     1501 ctaatatgga gatgcacgac tggtatgcaa acattgccag gctaagagga gcctacaagg
     1561 tcttcagcct gggaagtttc gaaaccctgc tgaccgatga tgaaaccggt gtgtacgtct
     1621 atcgccgaag actggaaggc gaaccggatg ctgttgtgat actgaacaac agtgagcagg
     1681 atcagcaggt cgatatcagt cagtggctga agggcacggt gtacaacccg atgaaaaaag
     1741 tggaacaata ctttccatct aaagatccca tggtcacagt tccctacaag agcgggctgg
     1801 tgttgatgag ttatacaaag taagagggaa aattattttg caacaggaat agtaaaattt
     1861 tagcacatca gctgaacaac tatatcatat ttattaatgt aagtatttta cttagaggag
     1921 aacggggctt agggcatagc aaattgcttc actgggatca gcaacaggtg ccatggttag
     1981 tatttaagaa atctgccaac tggctcttaa aacccactca caggctataa tagtagctca
     2041 taatcatccc agtggtaccc ttcgcccgtc tgagaatgac ctgaagctga cacaaaagct
     2101 aaaacaggta ggagatttga tagatgtgag gctccttgat cacatcatca taacctctga
     2161 gagctactat tcttttgctg atgaaggaac tctgcaaatc taaacctctt attattagag
     2221 gttttttgtt aatgtaagtt ataggtcaat ataaagtaaa attgtgataa ataaaggaaa
     2281 aattgataaa agtcacctga attatttttt atatggctaa cttttgtgat caagtttagt
     2341 aaaaattagc gttttcacaa agaaatatgt tgagtaaatg acatcaatca ttcaacagtt
     2401 agggtttagt cagcttcaga ttagtagctt gataagtctg ttatcagtaa ctttttgtgt
     2461 aaatggtcag gttgcatttg gacctgtaat tgaatcatct gaatgtttaa gtagtcctgg
     2521 gaatgatgca ttcgtagatg caataaaaag taaagatggc ggaatcttgg ttttaggata
     2581 tttgcgggaa tctgttgaag gaattatcat accagaagga tatctagctc caacggtcgt
     2641 aatcaagaag aatgctgacc ttgagaatca atggattcaa tttatcgggg gatctgacct
     2701 gggtacatcc gggcacacaa tattggaaga tgaggacgga tcaatttatt gtattggcac
     2761 aagtggtgcg aatacaggag acatcctcgg gaataatggg gttaaggaca tctttattac
     2821 caaacttaca tctgaaggag acaaactctg gacaactgtt attggaagta cagcaactga
     2881 tgaagttacc agtgctggct ttacctccac aggtgatatt ctggttaccg gttacagctt
     2941 tggcgacggt ggtgatatcc cttatcacta tgcttccttt accagcacgg atgtgatcta
     3001 tttttctatt gatacctccg gaaatctgaa ctggctgaag gtatttggcg gaaccggtgt
     3061 ggatcagcct gttttcaata ccgttctgaa taatgatggt aatacgctca tctgcattgc
     3121 gagcagttct gccgaccatg acctggtacc atacggactt actgataact ggaagttcat
     3181 catgatggaa gtggatgcgg atggcaatac gatctattct accggcatgg ataatgacag
     3241 cacgctggag gtttttgaag gtgccatgct cttaaaagag gatagtatgc tggtattcac
     3301 taccatgagc agatatgccg atctggcagt ttccaaaggc attgaagatg gggccatcgt
     3361 ggtttttact cctgaactgg agctcgacag gatgtacaac tacgggggct cgaactacga
     3421 ctacctgctc agggccatct atgagccaac caaacaggtg tattatgtgt tgggcagttc
     3481 atattcgatc gaccaggatc ttccgggcaa ctactccggt gggatcgggg atgatttctg
     3541 gttgatgtca gtggatgagg aatttaacct gctgtggagc cgaaactttg gtggggcaaa
     3601 tgacagcagt tcctttgggg atgcagcatg gtatgtcccc ggtaatatga tctatttaag
     3661 tgaaaaactt catttgttta caagctgtta tccaacagat aatccaccgg actttgattt
     3721 aaactgcaca ccttcaacag gcatttttac taccactgat ggctggagag taaccttcga
     3781 cttgctatca atggacatta tatcagatca aggaaaaaat gacattatta atatatatcc
     3841 taacccagct aatgatatca ttaatattag ctgcattgat tgcaccaata tttcagccat
     3901 tcgtctgact tcagtatatg ggcaagattt gttaatgaaa aattccaact gtaacgaaga
     3961 gttcgagatt gatgttagtg gtttttccag aggtatttat tttatcatcg tgagcgtaga
     4021 tgctgcaaat tttatatatc caataatatt aaactagtca catgagaaca gcaattatat
     4081 tatgtttatg catatcaata tcattttgga atcatcaggt caaggcacaa tcagtctgtg
     4141 gagtcacatc tttgatgacc tccgattgga ccagtgtagg ccccaattcc ttcacaaatt
     4201 ccacaatggg acgtgccaca gcgctttccg ttgaccctaa taacacaaat gatatagttc
     4261 taggtacaag atcaagtggt atctggaaaa ccacggatgg aggaagcaat tggatcaata
     4321 ttaccggatt taccaaccca ggtcttggaa ttgaccgaat tgttagatac aacacaacaa
     4381 cagacgtatt gcttgcgaca actcaattta atggatcaga tgtcagtttg tattcagctg
     4441 gactatttaa gtcatcagat gcaggtggtt catggtcaga ggtcagctct taccctagtg
     4501 aatatgaatt aactggatat ccatggttaa ccgctttagc ttcttctgag tcagatgact
     4561 tgttttatat tgggaacaag caaaagatgt tttcaataag cacgggttgg tcgtggcttt
     4621 ccaaaccagg ctatccaagt gaaaaggtgt ttttagactt tgatgatatt tctaccgacg
     4681 cttcacaaaa ttctcacacc atatcagata ttgacttaaa accatcggat catgatttta
     4741 tagttgtaag tcctagaaat ggaacataca ccgactatgt ttattcaaaa gatggtggag
     4801 aaacatggag ttcctttaat attccagttt cgccttctat tccatctgga tacgatgggt
     4861 atgaaatgga aatcaagact catattatgg atgatgggga tagttatgcc atattagtct
     4921 ataaatacta tagtaacact tctagtacaa cttcatatga ggcttatatt tttacctatg
     4981 atctagactt accgactcca tattcaactg ggacttaccc attaacaagt atggattaca
     5041 aaggagttgt cacagatttt tttgtaattg atggcgacgg ttctacagta ggtgacatat
     5101 gtattttttc tttgtctcaa agaaatgtta agcggactga acttcctttt tctcctggta
     5161 gtgctaccgt tagtgcaatt tcatcatatg gggctgagga tcctggcact catatcgatg
     5221 taaggtcaat ggaagtaata tataattcaa gcagcaatga ttatgaagca tttattgccc
     5281 acgatggtgg tataagcaac tgcgctgata ttgagaatgc tgtgggggac gatgattgga
     5341 ctaatatcaa tggcaatgga ctgcatatta ctgaatttag gggatttggg gtatctgagt
     5401 ttgaaaatgg aagaatcctt gcaactgcga tcgatggaaa tggattttta tttaaacgct
     5461 cactaactta tccatatgaa atagaaatat tagaaccgtt cggcggagat aattatagat
     5521 cagaagtatc aaaccatgat agagatatag gagtatttgc ttacaatgga accggtacta
     5581 aatcagatgc aagtattagt tcgtttaatc taagtacagt agcattatta aatgacctga
     5641 gttttgaaat accagatgga ttgaccacac cagataagac aaaatgggaa tataactatg
     5701 ctcttcgacc ttttgacttc gatgtttatg gctctgtatg ggcagggaat ttggatattt
     5761 atgaatacga atatgaccca ttggcgaccc atgttatctc aactaatggg gatttgcgaa
     5821 cagaaacttc aattcattgg ctttcacccc catcccctgc aactaagaac ccaattagtg
     5881 cttacaaacg tgttgatgac tccaacggtt ctgatgttat tacatggttt gcaacaaaaa
     5941 aagagcggct tttggggtca ccatatgcag atcctgtaaa gatagtgtct caagcttacg
     6001 atcgagatgc tcaaagtatt tcacctgcag atgacatcac tcccacattt gatgaagatg
     6061 gaaactcatc aaatggaata agaaaccaac ttgattattc atttgtatca tgtattgaaa
     6121 ctaacgatga tgacccagaa ggtctttggg tttcatttgc aggtaatacg cagggaggtc
     6181 acggtcaaat cttagaaaat gtactagaag atggcgatac tgcaaggttt ggtagaatct
     6241 attactacga tctcgctact gaggaatggg aaaacagatc gacaggactg cccaattacc
     6301 cggttcattg cttagagtat tggagaggaa gtgatgatgt agtttttgca ggaactgatg
     6361 tcggagtttt tgtttggaac gcaagttcat ctcagtggga atgtttcgat accggagatc
     6421 aactgcctta ttacagtatc aacaagttgg agattaacta ctgtacaatg aagctgtatg
     6481 ctagtgcctt tggtggtggc ctgtgggaaa ctgatttgcc gattggcttc cctggaagta
     6541 ggccctacac aaatgccatg gttattagca gtgatgttac atgggaacag gattttgatg
     6601 ctcgagtcga cattgttgtt aaaccggggg cgtccctgac aattaaaaat tgtgaggtta
     6661 gaatgcctaa ggatcatcgc attgttgtag agaggggcgc agtgctatgg ttagataatg
     6721 cagtcattac aaattattgt gatcaatggg taggagttga ggtctggggt aatgaaagta
     6781 agccccatcc aactagtgct tcatccatta tagccgggac ctacccctca gccactgatg
     6841 accagggtgt ggttgtcatg ttaaatggta gtgtaatatc aaatgctaaa attggcatcg
     6901 gcacatacag gaataatgga ggtgttcttg gaccgggtta taacggaggt attgtaattg
     6961 cagaagatgg aaactttcta aacaacacag ttggagtcaa atttatgccc tatgactatc
     7021 ctgaaacaat tggtggctta gattacgaaa acacggatga tgataatgtt tcatactttg
     7081 aatattgcac ctttaccaca aatgacaaga ttttcggaga tggccatcaa gactatcaag
     7141 tctatatgga cgatgtagac ggtgttcagt tctatggatg tactttcgag aatgaaattg
     7201 acgaaaccac actttcaaat ctaggatcgg gtatttactc agctgatgcc acctattatg
     7261 ttggtgctgc ctcatgcggt acttcttgtg aagactcatc cactttcaga ggctacttcc
     7321 gagctattga agctgccaac gtcgcaagcc tcccagtatt agatattact attaagaagt
     7381 cagtatttga agagaatcaa cgtggaatac tattgagcaa tgtgcttcat tcatctgtta
     7441 ttcaaaacag gtttgaggta aataatgcat cttcaatgca aaactatggc ttgtaccttg
     7501 aagactgtga agattatgaa gtagaggaga atctatttaa aggggttgga accggtggcg
     7561 agagtccatt gagtgccggg atttatgtag caaacaactc taatgcagtt acagagatat
     7621 atcgaaacac ttttagatac cttgagtcgg gaataagaag tcagacagtc aactcaaaac
     7681 ttcaaattaa atgcaatgta tttgacacag atatctatgt atacaatatt gcagtgacat
     7741 caggtggcat ccctaatcag ggatattgca ctggaaatgt agcagacccg gcaggaaatg
     7801 tctttagcaa tgattttata tatgaaggtg actataaggc acagacaggt gtggcaacct
     7861 ttaagtatcg tcaccataca gaccttgatc caatgtacta ctcatcttct ctcattacgc
     7921 tacaagattg taatatttat agcacatctg gagatgcctg tccgaccaat ttccctagcg
     7981 gtggaggtgg tgaagaatct tcatctatga tgatgtccga agcgggttca atggactctg
     8041 aaatcgatgc aatcatgtca tccttggcag gatttggggg aatggaactt ggagaatcag
     8101 aggcctatga cacttttatg gaggagctgg cttattatca ggataaaaag gagacgctac
     8161 tgaaggatgc ttatctgagc tatgttatgg aaggaaagat ggattctgca gaacaggttc
     8221 tattaaatga agctgaagta tgggctaaaa cttatctgat ctcccattac atcaattctg
     8281 gaaaattcaa gagtgcttct gaggtgatca aaagcatcga gacatcagat gatcagctaa
     8341 ttgattttct gaatttctct caattaatta tcgaactcaa atctactgga cagacactca
     8401 catccgttac tgatgataat ctggctcagg tagaagcctt gtcaggaaag cagtcacctg
     8461 caggtgtagc ttgtgagaac ctgttacagt ctatcaatgg tatagatatt ccggaagtat
     8521 ttgatgagat caccactttc gaagaaggtc ggacaagcca ggcagaggca attgaaagta
     8581 tggtctcaat ctatcccaat cctacagaca aacagttgtt tgtcagctta aatggggtgg
     8641 gtgaagaaag cgatgtttat ggtatatata ctatatctac tacaaatggt aagttatcac
     8701 tatctggaat gttaaaagac caactctcca cattagatgt ctcgatgcta tcctcaggtc
     8761 tttacataat tacaattacc ttgaatagca atggaaaaat acatagtgaa aagttggtta
     8821 ttgaatagta tgttagagta gatctgtgac tattgattgt ttattaacct acattgatct
     8881 taccggtcag gtaaacaaca gcctgtccgg taataagcac ccggtcgtct tctatggctg
     8941 tttgaaattt cccgatgcgg gaggagagtt gcaaggagat ccaggtttca cgttccagtt
     9001 tcttgctcca gtagttggtc agtgtggtct gcgctgaacc ggtagcagga tcttcattga
     9061 tgccgacaga cggcgcgaag aaacgatata cgtaatctac atcgtaccct cgtgcagtaa
     9121 caataatgcc atgcgctttt acctctgaca atagctgaaa gtccggcttc aaacggcgga
     9181 tatcatcttc actgtcatat atcagcaggt ggttggtctt gcctatatag gcttctttgg
     9241 gtgccttgcc caatgcctgt accatcttgt ctggcgctgc ctgatcaagg tagtagtcca
     9301 tggggaagtc cagggtgagg tcgccgttgt tgcgggttac acggagggca ccgcttttcg
     9361 attgaaagat gatataactg accggatgac ccagcatgtt ccagatgaca taggaagcag
     9421 cgagggtagc gtgaccgcag agatccactt cggtgacagg cgtgaaccat cggatatcaa
     9481 atgcatcacc acggggaatg aaaaataccg tttcactgag gttgtgctgt gcggcgatgc
     9541 gttgcatctg tgcatcgctc agccattcct gcaggggtac cacagcagcg gtgttgccac
     9601 caaacacatg atccgtaaaa gcatctatga agtacacgtt catgggagca tttttttaag
     9661 catgattatt tgaccgaagt ggtaattatc gtggtgcatc aggccatgga tgacggcata
     9721 ccaggtgtat ttgccatcgg cgggagcata gggtctgtcc agatcttcgt ctgataaagt
     9781 ggagatgaaa ttcctccagt ctgtatccac ctgttcaaag tcggccagga gttgttccca
     9841 tgcctgagga gaaacgttgt ccggttcaga gaggtagttg tcgtcgggtg atctgaatgc
     9901 ttccccctgc atcttccgca gtacattcct tctccagcca ctgcaatgct gtaccagttg
     9961 ccagatgctg ttggcattag gaataggatg gcaggcagcc tgcacagcat cgacatcttt
    10021 caggatgtct ttcaatcgta tccatgtcca cgggtcaccg tcatacagtt gttcccacag
    10081 gtgctggata cgggcagatt ccatgcccca aatatagaat aaggagtcat gctgtttcgg
    10141 ttagtttttc accacactgt tagaaacaac agaacggaca tgacatttaa cttcacccca
    10201 tggtctgttt acctgtccgg gcaggggcca ttcactactt taaaacttcc agcagaagtg
    10261 aatgccatgc atttctcttc taaagttacg aggtgat
//
LOCUS       DAOMAX010000072        10174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_894_length_10174_cov_3.493428, whole genome shotgun sequence.
ACCESSION   DAOMAX010000072 DAOMAX010000000
VERSION     DAOMAX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_894_length_10174_cov_3.493428"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK742_05510"
     CDS             complement(<1..368)
                     /locus_tag="PK742_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76081.1"
                     /translation="MHLLDYPCKKVLFLQMLEHLFRPHDQAWLKQCMRFYMAPYPMIS
                     ISAWNIEWLKQAGRPDVEYYVGNGVNFDQFPKEQKDHDNPVVLVEGLQPGNPSKDASR
                     QAWRVARALMREGGTGTGYG"
     gene            complement(575..1201)
                     /locus_tag="PK742_05515"
     CDS             complement(575..1201)
                     /locus_tag="PK742_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76082.1"
                     /translation="MVTISNVVSVNRDTGRSGDLPLNEELIRYAWANPADSGQTYLQV
                     SRGRMSERWLVDDAIADVISNLNTDAGREVLVAATLRKFRGETRTGDFAFAARNVVEM
                     MDDKDGYAVIFIGDEGEDKYLKVVVEEASADLQTEANTTLDQIESVQVQKINGVDQGA
                     SHPVIYFSSRYIVDYTPASGNAEARYLVKPNEVMGGKEYEVYTAGSSS"
     gene            complement(1249..1797)
                     /locus_tag="PK742_05520"
     CDS             complement(1249..1797)
                     /locus_tag="PK742_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76083.1"
                     /translation="MAVTEATIVNSKNVFRKDGSRKFEARAELLSTSTSIYANFNVVG
                     GLVKIPLGKVQNGIIHGVRIACDKVNAFSAVLAYFFRGEVDEIADHAAYGFAADQYEN
                     NKKLLGSVSVSLSNPGSSVIDTGSSTGIDLDFLADVDPDDTEANPELQERAIYCYLLA
                     QSGGVAPAVGTKINLEVHYCGW"
     gene            complement(1784..2077)
                     /locus_tag="PK742_05525"
     CDS             complement(1784..2077)
                     /locus_tag="PK742_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76084.1"
                     /translation="MELQAMLFAFLGVLFYILVEIRKVAGPGFSPSYWMKRNGWFSLS
                     SLLISVLLGYILPTTGQIPDVSDERFVNFTFFCLGAALDPFLKTFKPLNNGSN"
     gene            complement(2087..2656)
                     /locus_tag="PK742_05530"
     CDS             complement(2087..2656)
                     /locus_tag="PK742_05530"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQU76085.1"
                     /translation="MSQIPDSMWGEFITWFAKVVAGAVAIWKGIWPALKWAWNWRKRR
                     REARKRLEKDVTEIKNMAHVMLAQMEVFRDNQIAQGEMKLQHLDISDKMVVMLSQLGE
                     LEFANEAFLKFVDKDLPDLEHSNWISSIVHQEDRQAVREYLNDCVNNKRRFKYEFRLN
                     LNGHGVHLVEFEGKSGEKAGYFINVKQKQ"
     gene            complement(2686..4014)
                     /locus_tag="PK742_05535"
     CDS             complement(2686..4014)
                     /locus_tag="PK742_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76086.1"
                     /translation="MRRLLFAIALLLSIPAYSQLGIATEIVGRKYYGSTQLYGDTIWA
                     INGPITFGIAHDLNLYRSSSTVLRTDDYFRAVRIGIGADPTTIFYAAGTDAGDYATYD
                     GSGLEIRLTTVPPTPEPWLEVYDDNGVAMFSGIIDELGNGGIAGRNDAGLEIWKLNGY
                     LNGHSFFNQEYFSIGRNGVVSAYGGTLAGGMLTVNNSRFDRDPAADLNNPNEYMIVVE
                     DEDNDGDWLGVGFISGGTGTYEDNIGAALIYENTGSAGQGVLHGYAKSSTTDAADPDL
                     IFTAGNEVFALDYIPSATEDNSDSIATFDAYGRIGWKTFIGQSILQGVPDVTVAAGST
                     VYTVFNQTSTAVEIRRQLIIPIDGTIKKLYVTTSSAQPGTGTATATIRINRVSSSVVA
                     TIGSGASAGTFTDLSNTVSVSAGDLVSIKLTNNAATTSARIISVACVLEY"
     gene            complement(4011..4763)
                     /locus_tag="PK742_05540"
     CDS             complement(4011..4763)
                     /locus_tag="PK742_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76087.1"
                     /translation="MATTHKDGSYFRRYSNAVASYPEATAYEATVVSTGGTDLDLRGA
                     NPPQKVNNKWLLIKNGYQSFVAKITKSTFNYIKVDRDVLAGEWDVFVIDDNWSLPVTI
                     SNDGVKYVFINGQVLKQNGRVRFSKQSNDEPIVMYGTDTWLITNGEVEQREYFDVGSG
                     SQAIPGDPGTVDAFIPETITAEYIEDSRLMPFTGRVEITHNSDILIPDLHYTVEDVDL
                     LLNRIYVKEPESGHPAFNFDTTGYILVKLYYS"
     gene            complement(4747..10050)
                     /locus_tag="PK742_05545"
     CDS             complement(4747..10050)
                     /locus_tag="PK742_05545"
                     /inference="COORDINATES: protein motif:HMM:NF037529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76088.1"
                     /translation="MGSSKNEIPGVEKRLEYAKRMISGDIQAKVQALVDKLGKDDMIL
                     DKDLGPFAEKATEPQSTLYDQLEQLKKKEAADRPLFEAFIERQMQYSLSAVYHIESVF
                     PDHSPEKVNDLVEAWVRNMFQHDKMVTEAQGEPVPEMPFLKADGEGPGAEVVTQEEVE
                     GILGLKGVDYTEAGSTEDLDDDDGAADIWASEAEDINNEETRATYGSGENYENPTYNT
                     GDVEIEGEVEGEKTTLPTEAALESVINRIRKLYPSLDIVVTDAIPEGANPVFFQNGSM
                     HINPALIDADGMVSQLATMWLAIFRGFNPAGYNQISQIYREENDGADLDESVVFQEIA
                     DAVAAGKEPTGFEKVWQWIKNFLAGIFGYFPVDISKMTVKQFANLAARELWTGKGVAD
                     IRANGVASALLNTKGAFAKFQRSRLMPSGERFSTEYGPDSVVANMNGLKVRLSDVSRR
                     LIEGMTYQVGGGVRLGYKGLRILGRTHLGHFIESNRQVRIKGNLTAIQTIAQEIGHAY
                     DMAVAKFTDTLYFHVKNDGKVEIEQPGDSGLNPGDMKSLREVNKMNKKLQRAGRTMAT
                     YKNVVDANLAAIGKQLIGWDAARVESSKGYSNLDIEGKIKEAIAEFVSEYVVGEHPVP
                     VQSLMFYDMFENQLEMMASQGSPEYRNALRSARVEYQVYKAMPALWRQMTNLNTIKDG
                     DLYSVETKSVMSQWLQKGKNVIGYARKKQLQYEIAMTDDAFVMKHLHYSLKAIGKAPK
                     KESAALKEIQKNINYYIRALYGTYGMANAMLHKPFRRRVVHGIPQLPRSTGERSLHDV
                     ISDFNDDGLLNSFESYLMAKRQLVYADRGMLQEDGRAASGEYIQSLREAVADFEALAG
                     RRRAREGAEAVYQFQAHGLQYLVDSGLKTQDEMDRMMILNPFYVPIAADKHVDTAPAE
                     FYGGTSEDLDLRTNDPLRNIKPLDIKDMDKWESPMDMIAKNMVRQIHAANKGLMNWNT
                     VEYLHEIQKMIPDMGDNWIQRVPETDVVMDGYDIHYEDGVEVDRTPRFRRKHVDPSVK
                     EKAVVIRVINKDAYTKGGQKKTGGGTNTLEVVELDEFGQPVLDEDGNEVMTTMQVTKP
                     TYYQVPAELFELINFGHQYNLPIPQLARMAGELLRNGAINMNPVFSFLRNPFRDFSSA
                     TYNVESKIPYNAVDLFRALAKNIVDAATTDETMTPLEWLTSASGADLSSSVYGSSFRE
                     NIVREAQKGLLRKGWDGANPVGEDFILRRAARWSDSINRHGAFLKLYREGADVRDMVA
                     AFREASGDYAVGGKIPKAVAALYPFMKIQVWFARKARYILTDAAKDPRKLGRFAAVSM
                     MWMGLEMACWVAMQQGFPGEDDKDRERRRTRYRNSQPWLKYVTMGVPLPGGGIMFLPK
                     STFQMWLGSSMTFALEKAQGADPGFTYKNLRTMMADQHFYISTSKDENGLRYGFYNII
                     PHVARPVLEGLSNYNTFQRRPIVPYYMDKLDKQAQYYDSTPYVYRQLGLRFDISPLVL
                     QHLVESYTATVGRFAGKVFSAWLFDQVNDYDYSEGSAWRQVVVPNMLSEAYDHLGRNS
                     YPVSKAYDLFDKVETAQSTFDKEYESYLEYVLDSPVRNDQVESQYVASMLKTLKDPYV
                     RLALFFKAKQISTGDKIGINGQWVTDSNLKSIKDSIAAGASLEDIDISLGSSGWLKGS
                     GGVEGVLKNGFAIIKEFQRSPDPEMRAWAKQQEVEITKLFADFYAGVTVELEKMKADE
                     NYVPYDDSGLLKGAMLEPLKKLQEQVQRMDDFQVKQHGNNP"
BASE COUNT         2269 a         2850 c         2326 g         2729 t
ORIGIN      
        1 atgccatacc cagtccccgt cccaccctcc cgcatcagcg ctctcgccac tctccacgcc
       61 tgccggctgg catccttact gggattgcca ggctgcaaac cctctaccag caccacggga
      121 ttgtcatgat ccttttgctc cttcgggaat tggtcaaagt tcacgccgtt gccgacatag
      181 tactccacat ccggacgacc ggcttgcttc aaccactcaa tgttccaggc ggagatactg
      241 atcatcggat aaggagccat gtaaaacctc atgcactgct tcaaccatgc ctgatcgtgt
      301 ggccggaaca aatgttccag catctgcagg aacaatactt tcttgcaggg atagtccagt
      361 aagtgcacat cgtgaggact ggtcagtatc aagagatcgg cccccgccgg tgacgacacg
      421 acatcacacg acaaggggcc gaaccaacca aaaacatgac cctttaacgg cagtaggccc
      481 accgagaaat tctcgctgag cctattcgcc cactccagga taactctgac tcccccgtga
      541 ggtgaatcag ttggagttgt tatgatcacc cgcatcagct ggagctgcct gcggtatata
      601 cttcatattc cttgccgccc ataacttcat taggcttcac aagatacctg gcttctgcat
      661 tgccagatgc cggagtgtag tccacgatgt atcggctgct gaagtagata acgggatgcg
      721 aggctccctg atctacgccg ttgatcttct gcacctgaac gctctcaatc tggtcaagag
      781 tcgtattggc ttcggtctgc aggtcagcag atgcttcttc taccacgact ttcaggtact
      841 tgtcctctcc ttcgtcaccg atgaagataa cggcgtaacc gtctttgtcg tccatcattt
      901 ccacgacatt ccgtgcggca aatgcgaaat caccggttct ggtctcccct ctgaacttgc
      961 ggagggttgc agctaccagg acttcacgac cggcatcggt gttgaggttg gaaatgacgt
     1021 cagcaatggc atcatctaca agccagcgct cgctcatacg accacggctg acctggagat
     1081 aggtttgacc ggaatcagcg gggtttgccc acgcatacct gatcagctcc tcattgaggg
     1141 ggagatcacc ggaacggccg gtgtctctgt tcacggaaac tacattggaa atcgttacca
     1201 taattgttat ttactggtgt tttgcgaggg tcctaccctc agttcatatt accatccgca
     1261 atagtgcact tcaagattga tttttgtacc aaccgcaggg gcaaccccgc cagactgcgc
     1321 taacaagtag caataaatag ccctttcttg cagctcagga ttagcttctg tatcatccgg
     1381 atcaacgtca gcgaggaaat ccaaatcgat gccggttgac gatccggtat caatgaccga
     1441 gcttccggga tttgaaaggc tgacagaaac cgaccctaag agctttttgt tgttttcata
     1501 ctgatcagca gcaaatccgt atgccgcatg atcagctatt tcatcgactt ctccgcgaaa
     1561 aaaatatgcc agcaccgccg aaaaagcatt gaccttatca caggcaatcc ttaccccatg
     1621 aataatgcca ttttgcactt tgcctaaagg tatcttcacc aaacctccaa ctacattgaa
     1681 gtttgcatag atgctggtgg atgtactgag aagctctgct ctggcttcga atttgcgaga
     1741 accgtctttc ctgaagacgt tcttcgagtt tactattgtg gcttcagtta ctgccattgt
     1801 tcaatggttt gaaggttttg agaaacgggt ccagcgcagc tcccaaacaa aagaatgtga
     1861 agttgacgaa cctttcatct gatacatcag ggatctggcc ggtagtcggg agaatatacc
     1921 cgagcaaaac cgatatcagc aaactgctga gggaaaacca gccgtttcgc ttcatccagt
     1981 acgatggact gaagccgggg cctgccactt tcctgatctc taccaggatg tagaaaagga
     2041 ctccgaggaa agcgaataac attgcctgta attccatagc ttaaaattat tgtttttgtt
     2101 tgacattgat gaaatatcct gccttctccc ctgacttgcc ctcaaattct actaagtgca
     2161 ctccatgacc gttgaggttg agcctgaact catacttgaa tctccttttg ttattcacgc
     2221 agtcattgag gtattccctg actgcctggc ggtcttcctg atggacaata gaggatatcc
     2281 agttgctatg ctccagatca ggtagatcct tgtccacgaa tttcaggaag gcttcattgg
     2341 cgaattccag ctccccgagt tggctgagca tcaccaccat cttgtcgctg atgtccaggt
     2401 gctgtagctt catctctccc tgggcgatct ggttatctct aaacacctcc atctgagcca
     2461 gcatgacatg agccatgttc ttaatctcgg tcacgtcttt ctccagccgc ttccgtgcct
     2521 cacggcgacg ctttctccag ttccacgccc atttcagtgc aggccagatg cctttccaga
     2581 tagcgactgc ccctgcaacc accttggcga accaggtaat aaactctccc cacattgaat
     2641 ccggtatctg tgacattggc gccatttgtt tctttttgat taaggttaat actctaatac
     2701 acacgctact gaaattatcc ttgcagaagt ggtggctgca ttgtttgtta gctttatgct
     2761 gacaagatcg ccggctgata cacttaccgt atttgacaga tcggtaaaag tgcctgcaga
     2821 tgctcctgac cctattgtgg ccaccaccga tgagctgacc ctgtttatac gaatggttgc
     2881 agtagcggtg cccgttcctg gctgtgccga agacgtggtg acatatagtt ttttaatcgt
     2941 gccgtcaata ggtatgatca gctgcctacg gatttcaact gccgtgctgg tctgattaaa
     3001 cactgtataa actgtggatc ctgccgctac tgttacgtct ggtacgccct ggagaatact
     3061 ttggccgata aatgtcttcc atcctatccg gccgtaagca tcgaaggtgg ctatgctatc
     3121 actgttgtct tctgtggctg atggtatgta gtccagagca aatacttcgt ttccagcggt
     3181 aaatattagg tctgggtctg cagcatcggt agtgcttgac ttggcgtatc catgaagtac
     3241 gccctgtcct gccgaacctg tgttttcgta aataagagct gctccgatat tgtcctcata
     3301 ggttcctgtt cctcctgata taaagccaac ccccaaccag tctccgtcat tatcctcgtc
     3361 ctctacaacg atcatgtact cgttggggtt attcaggtct gcagcagggt ctctgtcgaa
     3421 cctgctgttg ttgacggtga gcataccccc ggcaagtgtt cctccgtatg ccgatacaac
     3481 accgttccgg cctatgctga aatactcctg attaaagaat gaatgcccat ttaggtagcc
     3541 atttagcttc catatctcca gtcctgcatc gtttctgccg gctatacctc cgttgcccag
     3601 ctcgtctata atgccggaaa acatcgccac accattgtcg tcatatactt cgagccaggg
     3661 ttcaggtgtc ggcggtaccg ttgtcagcct gatttccagg ccagagccgt cataggtggc
     3721 atagtctcca gcatccgtac cggcggcata gaatattgtt gttggatctg ctccgattcc
     3781 aattctgaca gctcggaagt aatcgtcagt ccgaagcacg gtagaggagc ttcgatacag
     3841 gttgagatcg tgcgctatgc caaaagttat cgggccattg atcgcccaaa tggtatcacc
     3901 atacagctga gtgctgccgt agtatttcct ccccactatc tcagtggcaa tgcccagctg
     3961 ggaataggcg gggatggaaa gcagcaatgc gatcgcaaac aaaagtcgtc tcatgagtag
     4021 tagagtttta ccaagatata tccggtggtg tcaaaattaa aggctggatg gccgctttcg
     4081 ggttccttca catatatgcg gttcaacaga agatctacat cttcaacggt ataatgcagg
     4141 tccggtatga ggatatcgga gttatgtgtg atctccaccc tgccggtgaa cggcatcaac
     4201 cgggaatcct caatgtactc ggctgtgatt gtttccggaa tgaacgcatc caccgtgccg
     4261 ggatcgcctg ggatcgcctg gcttccagag cctacgtcaa aatattccct ttgctctacc
     4321 tcaccgttgg tgatcaacca ggtatcagtg ccgtacatga caataggctc gtcgttgctt
     4381 tgcttagaaa acctgacacg gccgttctgt ttcaacacct gaccgttgat aaatacatat
     4441 ttaacgccat cgttgctgat ggtgaccgga aggctccagt tatcgtcaat gacaaagaca
     4501 tcccactctc cggcaaggac atcccgatct accttaatgt agttgaaagt tgatttcgtg
     4561 atcttggcga caaaggactg atagccgttc ttgatcagca gccatttgtt attcaccttt
     4621 tgaggcggat tcgcccctct cagatctagg tctgttccgc ctgtgctgac aacggtagct
     4681 tcgtaggctg tagcctccgg gtagcttgct acggcattgc tgtatcgcct gaagtaactg
     4741 ccgtctttat gggttgttgc catgctgttt gacttggaaa tcatccatac gctgcacttg
     4801 ttcctgcagt ttcttcaacg gctcaagcat agcccctttc aggaggcctg agtcgtcata
     4861 aggaacatag ttctcgtcag ctttcatctt ttccaattca acagttacgc ctgcataaaa
     4921 gtcggcaaaa agtttggtta tctcaacctc ttgctgtttt gcccatgccc gcatttccgg
     4981 gtcagggctc cgttggaact ccttaatgat ggcaaatcca tttttcaaca cgccctctac
     5041 gcctcctgat cctttcagcc atccggaaga acccaggctg atgtcaatgt cttccagaga
     5101 ggctccggct gcgatgctat cttttatgga tttgagattt gagtctgtca cccactggcc
     5161 attaatgccg atcttatccc ctgtgctgat ctgcttggct ttgaagaaca gagccagcct
     5221 gacgtaagga tctttcaggg ttttcaacat tgaagccaca tactggcttt caacctggtc
     5281 attccgcacc ggagagtcga gaacatactc caggtatgat tcatactcct tgtcaaatgt
     5341 ggactgagcg gtctctacct tatcgaacag gtcatacgcc ttagatacgg gatagctgtt
     5401 ccggcccagg tggtcgtatg cttcagacag catgttcggg accactacct gcctccaggc
     5461 tgatccctcg ctgtaatcat agtcattaac ctgatcgaaa agccatgcag agaacacctt
     5521 gccagcaaat cttcccacag tagcggtata gctttccacc aaatgctgaa gcaccagcgg
     5581 ggatatatca aacctcagcc ccagctggcg gtacacatac ggcgtgctat cgtagtactg
     5641 agcctgcttg tccagtttat ccatatagta cggcacaatc ggacgcctct ggaaggtgtt
     5701 gtagttggac aacccttcca gcaccggcct ggccacatgc ggaattatgt tgtagaagcc
     5761 atatcgaaga ccgttttcat ccttactggt gctaatgtag aaatgctgat ccgccatcat
     5821 ggtgcgcaga ttcttatagg taaagcctgg atctgcgccc tgagctttct ccagagcaaa
     5881 cgtcatggat gatcccagcc acatctggaa tgtggatttc ggcagaaaca taatgccacc
     5941 tccaggaaga ggtacgccca tggttacata cttcagccac ggctgagagt ttctgtatct
     6001 ggtccgacgg cgctccctgt ccttatcgtc ctctccgggg aagccctgct gcattgccac
     6061 ccaacacgcc atttccagtc ccatccacat cattgacacc gctgcaaacc ttccgagttt
     6121 gcggggatct ttcgctgcgt ctgtgaggat ataccgggct tttctggcaa accacacctg
     6181 aatcttcatg aatgggtaca gggctgcgac cgctttcggg atcttgccgc caactgcata
     6241 atcgccactg gcctcacgga aagcagcaac catgtctcgg acatccgccc cttcgcgata
     6301 cagcttcagg aaagctccat gtcggttgat gctatctgac cacctcgcag cccttctcag
     6361 gataaaatct tcgcctaccg ggtttgcccc atcccaacct tttctcagta agcccttctg
     6421 cgcttctctg acgatattct ccctgaaact ggatccgtac acgctggaac ttaaatcggc
     6481 tcctgatgcg ctggtcagcc actccaaagg agtcatggtt tcatctgtag tagcggcatc
     6541 cacaatattc tttgccaggg cacggaaaag atcaacggca ttgtacggaa tcttgctctc
     6601 cacattatag gtagcagaac tgaagtcccg gaacgggttt ctcaggaaag agaacaccgg
     6661 gttcatgttg atggcaccgt ttctcagcaa ctcgccagcc attctagcca actgaggtat
     6721 ggggagatta tactgatgac cgaagtttat gagttcgaac agctctgcag gaacctggta
     6781 atatgtcggc ttggtcacct gcatggtcgt catgacctca ttcccatctt catccaacac
     6841 cggctgtccg aattcgtcca gctctaccac ttccagggta tttgttccgc cccccgtctt
     6901 cttctggcca ccttttgtat atgcgtcttt gttgataacc ctgatcacca cagccttttc
     6961 ctttacgctg ggatctacat gctttctccg gaatctgggt gtgcgatcta cttctacacc
     7021 gtcctcatag tggatatcat atccatccat gaccacgtct gtctcgggta cacgctgaat
     7081 ccagttgtca cccatgtcag gtatcatctt ctggatctca tgcagatact ccacagtatt
     7141 ccagttcatc agccccttat tggcagcatg tatctggcgc accatgttct tggcgatcat
     7201 gtccatggga gactcccatt tgtccatgtc ctttatatcc agcggcttga tgttccgcag
     7261 gggatcattg gtacgcaggt ccagatcttc tgatgtaccg ccgtagaact ccgcaggagc
     7321 agtatctaca tgcttatccg cagcgatcgg aacatagaag gggttgagaa tcatcatccg
     7381 atccatttca tcctgggttt tcaacccgga atctacaaga tactgaaggc catgtgcctg
     7441 gaactgatat acagcttctg ccccttccct tgcccggcga cgaccggcca atgcctcaaa
     7501 gtcagcaacc gcctctctca ggctttggat atactctccg gatgctgcac gaccgtcttc
     7561 ctgcagcatg cccctgtccg catatacaag ctggcgcttt gccatcaggt aggactcaaa
     7621 actgttgagc aggccatcgt cattgaagtc gctgatcaca tcatgcaatg accgctctcc
     7681 tgtggacctc ggcagctgag gaataccatg taccacccgg cggcggaacg gcttatgaag
     7741 catagcgttt gccatgccat aggttccata cagggcacgg atgtagtaat tgatattctt
     7801 ctggatctcc ttcagtgcgg cactctcctt cttaggggcc ttcccgattg ctttcaggct
     7861 gtaatgcagg tgtttcatca cgaatgcatc atcagtcatt gcgatctcat actggagctg
     7921 cttcttcctg gcgtaaccga tcacattctt tcccttctgc agccactggc tcatgacaga
     7981 cttcgtttcc accgagtaca ggtcgccgtc cttgatggtg ttgaggttgg tcatttgcct
     8041 ccacaacgca ggcatggcct tatacacctg gtattcgact ctcgctgagc gcagggcatt
     8101 cctgtactcc ggagatcctt ggctcgccat catttccagc tggttctcga acatatcata
     8161 gaacatgagg gattgcacgg gcactggatg ttcgcccaca acgtattcac tgacaaattc
     8221 agcaatggcc tccttgatct tgccctcgat atctaaattg ctgtaccctt tgctgctttc
     8281 aacccgagcg gcatcccatc cgatcagctg tttcccgata gcagcaaggt tagcatccac
     8341 gacattctta taggtggcca tggttctacc ggcacgctgg agcttcttat tcatcttatt
     8401 tacctcccgc aggctcttca tgtcgcctgg gttcagaccg gaatcacctg gctgctcgat
     8461 ctccacctta ccgtcattct tcacatggaa gtacagggtg tcagtgaact tcgccacagc
     8521 catatcgtaa gcatgtccta tttcttgggc aatggtctga atagctgtga ggttgccttt
     8581 gatccggacc tgcctgttgg actcaatgaa atgccccaga tgtgtgcgcc ccaatatcct
     8641 cagacctttg tacccgagac ggacaccgcc ccccacctgg taggtcatgc cctcgatcag
     8701 gcgccggcta acatcagaca gccttacctt caggccgttc atgttcgcca ctacggagtc
     8761 aggcccgtac tcagtgctga acctctcccc gctcggcatc aaccgagatc gctggaactt
     8821 cgcaaatgct cctttggtat tcagaagggc tgaggcaacg ccattcgccc tgatatcggc
     8881 aacacctttg ccggtccaca gctccctggc tgcgagattg gcaaactgct tcaccgtcat
     8941 tttgctgata tcaacaggga agtacccgaa gatgccagcg aggaagttct tgatccactg
     9001 ccagaccttc tcaaatcccg ttggctcttt accggcagca acagcatctg caatctcctg
     9061 gaagacaact gactcgtcca gatcggctcc atcgttttcc tcccggtata tctggcttat
     9121 ctgattgtat ccggcagggt tgaagcccct gaaaatggca agccacatcg tagccaactg
     9181 tgataccatg ccatcggcat cgatcagagc cggattgata tgcatacttc cgttctggaa
     9241 gaaaacagga tttgcgccct ccggtatagc atcggtcacg actatatcca ggctcgggta
     9301 cagcttccgg atgcgattga tcacgctctc cagtgcggct tcagtaggca gagttgtctt
     9361 ttctccctca acctcaccct cgatctcaac gtcaccggtg ttgtaggtag ggttttcgta
     9421 gttctcccca ctgccatagg tggcccgagt ttcttcattg ttgatgtcct cagcttcgga
     9481 cgcccagata tcagctgcgc catcatcatc atcgaggtct tcggtagatc cggcctcagt
     9541 ataatccacg cctttcagcc ccaatattcc ctctacctct tcctgggtga ccacttctgc
     9601 acccgggcct tctccatcgg ctttgaggaa tggcatttcc ggtacgggct ctccctgagc
     9661 ttcagtgacc atcttatcgt gctggaacat atttctcacc catgcctcca ccaggtcatt
     9721 gaccttttcg ggagaatggt cagggaatac gctctcaata tgatagactg cggacaggct
     9781 gtattgcatc tgccgctcaa tgaaagcctc gaatagtggt cggtcagcag cttccttctt
     9841 cttcagctgc tccagctggt catacaatgt ggactgaggc tctgtggctt tctctgcaaa
     9901 tggccccaga tccttgtcaa ggatcatgtc gtctttaccg agtttatcca cgagcgcctg
     9961 tactttggct tggatatccc cgctgatcat gcgcttggca tattctaacc ttttttctac
    10021 gccggggatc tcgtttttgg aagaacccaa atcacgactg acaagattcc gaagccgctg
    10081 gatggttgta gtcatccgtt gtgttggctc cggagactcg ccccattctt tctccccgac
    10141 aatgcgctta tgcttatacc ctttggaaaa taaa
//
LOCUS       DAOMAX010000073        10158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_897_length_10158_cov_4.168861, whole genome shotgun sequence.
ACCESSION   DAOMAX010000073 DAOMAX010000000
VERSION     DAOMAX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_10158_cov_4.168861"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1127)
                     /locus_tag="PK742_05550"
     CDS             complement(42..1127)
                     /locus_tag="PK742_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQU76089.1"
                     /translation="MRIAFDAKRIFHNTSGLGNYGRNLINSLEQYYPDNDYLLFTPRN
                     GRVPFKPASSRILYPDTNTPALWRSFSIIRQIAREQPDIYHGLSNELPFTMRSAKCRS
                     AVSVHDVIFRRYPEAYGIIDRNIYHYKTRFAIRHADVIFTSSQATANDLEQLYGADPG
                     RMVVLFPGVEQIFYEPAEPSAVTENLPTEFFLFVGLGNRRKNFEIVLEALSRQSESQR
                     IPIVAVHGPGKYASAMRLLTTQRKLEKWVHFIPSDNAGLRALYHQAVALIYPSLYEGF
                     GMPVAEALVCNCAVLASDRSSIPEAGGNAALYFDPEHADALADAMFRIQDDQFRANCI
                     QKGIAYRERFTPEVLAGTAMDAYRRLI"
     gene            complement(1189..3582)
                     /locus_tag="PK742_05555"
     CDS             complement(1189..3582)
                     /locus_tag="PK742_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HQU76090.1"
                     /translation="MHSSARIALLSLFAIGVWCTTPALALTCGASEVPVHIFIDPDSW
                     PGEISWDITDADGGIIASGDYNSDSTCVVDGECYTFHLYDSYGDGIYSPHGVELYYDN
                     ELVAFFTGDYDYGTSIDIGCPPGTACSSSIEVTEGTYTAPAPNSWYEFTPAEYGTYII
                     STCDLDNTCNTQLWVYDHCDGLVLSEYAEGTVYYNDDACGEQAEITGILLEGETYYIR
                     VGDLGSSCEGTSINWSLTYAGPPSGCTDPYACNYSPLATVDDGTCIYPGSPDCPAGPD
                     LVLDSTYFDGYPSFTSDFSLESYDSDWDDCAFEEACVTGTGIRYVLKFGVKIKNIGDV
                     DYHLGSPTDGAPGFVYSDCHNHWHYADYGEYLLYDSLGNEIPSGHKNGYAVMDVGCFD
                     GTPQYGGWNMGISAGCYDIYGRGTSCQWVDLTDVPDGQYTLVARINWENNPDIDGHVE
                     TDLSNNWASICILIERDEPGGEPTVTRVEGCTPYYDCYGEIFGSATYDCNGICNGPAR
                     IGDLDLDGTQTSDDVTQYTTEILDNTILAATCNDANNDSDIDVYDATLVNGCAWENLG
                     VTHVIELCDLPYGFNEYGDTVTYSIGEIDPGEGYLDIYSVNTTADILAFQLKILGITT
                     IDSVVPMAESIMSSDFRFAFSNDEIIGIGYDEIGYEKNYTPNPVARVYFQATYPTTIC
                     LDYITNAVNEAYHEIITAKDNGCFTLTGLEDAHAAYGLQVSIQPHPVTGVSVMRFSNP
                     HNDTFELRMLDLSGRMVSETETQSDHFNISNNEYLPGVYIYQLTNGTESLQGTVVIQ"
     gene            complement(3616..4008)
                     /locus_tag="PK742_05560"
     CDS             complement(3616..4008)
                     /locus_tag="PK742_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQU76091.1"
                     /translation="MAAVTRLHLYYLSTCDTCARIMKELQVDQFPFVLQDIKYEPVTR
                     KQAEEMAELVAQQYSSPPYESLFSKVALKYRSMGLNKMDLTEADYKKYLLQEYTFLKR
                     PVFIIGNRIFMGNAPKTILAVAEALHAF"
     gene            complement(4011..5222)
                     /locus_tag="PK742_05565"
     CDS             complement(4011..5222)
                     /locus_tag="PK742_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU76092.1"
                     /translation="MRINQPSTEQLQELLLDIYRQQDTDALQAIVLQKLVSFFGYDYA
                     VVLQQIGSPERYIRKQLFATEDRFGQSAVPLEFKVAIGSWPSQEWATSDVHPCTWLYH
                     DPLSTGHIGIGLPAPGTAYCVLAYSRNKPRMGAKTMDLFWRTVRSHYSTLGGTDILLH
                     SIAEPMPEMGQMELKLRQLEESNQELKQFASRASHDLQEPLRTISNYITLFLRNHGAG
                     LESEGREYLGFAKNAAERMHHLVKDLLTYTRLDHTSDQMTEVSCNKVLETALDNIKMS
                     VEESDAIILYEDLPEINGHEQQLVSLFQNLLDNAIKFRGDDDPVVMVQVEDAGQTYEF
                     SVCDNGIGIPDDFHDKIFQFFTKLHPASEYKGTGLGLSICKKIVENHGGQIRAESADK
                     EGTTFIFQLSR"
     gene            complement(5246..5845)
                     /locus_tag="PK742_05570"
     CDS             complement(5246..5845)
                     /locus_tag="PK742_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQU76093.1"
                     /translation="MNKRRSVRMFSSEPVLQEVLENIVMTASTAPSGANKQPWTFCVV
                     TDQKLKRQIRIEAEKEEKISYEQRMPQEWLDDLKPFGTDWHKEFLETVPALIVVFKRN
                     YETDADGHKHNNYYVSESVGLATGFLLAAIHQAGLVALTHTPSPMNFLKQILDRPANE
                     SPFLLIPVGKPHPDTKVPDLRRKHAGEVLVWYDLDRTDK"
     gene            5950..6567
                     /locus_tag="PK742_05575"
     CDS             5950..6567
                     /locus_tag="PK742_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQU76094.1"
                     /translation="MKIPESLQTVNAAAESVYQERSSRFIALVRPCSDDATARQFRDA
                     LKKEHHKAVHVVLAYRIGHTQAVEYSTDDGEPTGSAGKPVLNELRSRMLSNTAVCVVR
                     YYGGKKLGIPGLIQAYRQAASQALELAGSQELVHRMRIRVHVPASEQHLVANLISGST
                     ARIEDAVYAETTTFIVSLEHKDETVLQKLESHWQLAVERIADTDN"
     gene            6569..8155
                     /locus_tag="PK742_05580"
     CDS             6569..8155
                     /locus_tag="PK742_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76095.1"
                     /translation="MSAAAQQTTEAEALAAIRFPGYSFETNHVFQTDHATYTRLQPFS
                     AGIQVDRQVVIVQPYNGEMRVMGLLPPVSDLITSLVYTEACIWILQNDSWEAVQMSTQ
                     TDRHGHDRTDVFLNEEGDTISVRSWRSYFGDSTVTARVFRPDPITVAEAEYGGDLVDN
                     NDADDPVLNSLRSLVTVTATFEDDTFFLYDTFIRLKDFAAPVQPVAWSLTPVFDAGRG
                     DPAFEDVNTLYHLQQCRNWLVSLGFEELCSFEIDIDTHGADGADNSFFSSGGTPSIQF
                     GEGGVDDAEDADVIIHEYMHALSHSAAPFSNTGIERRAMDEGYADYLAASYSLQWSSY
                     RWQDVFSWDGHNEFWDGRTTDNDKHYPEDNSLNIYAASGIWSGALADILESIGKENTD
                     KLVLEALYGSYDEMTMPEAALWLLDVEDMLFGGLYHEEVFTILDTRGLLPSTGVQEQQ
                     LYNISFYNTNAFADGTGPLLIITDPGKQLLQMFDMSGRMVSSFCGEGPELEWMPPLTS
                     GSYLLTVTGRTTGSTMLIVK"
     gene            complement(8156..9004)
                     /locus_tag="PK742_05585"
     CDS             complement(8156..9004)
                     /locus_tag="PK742_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU76096.1"
                     /translation="MNRYAKQVLVPTDFSEESFAALEYAVFYANRTDSGIHLIHVANR
                     EMLRKQGGDVRTYRALLQDIRQQLRTFRNRSGGAFRIHADYKLSEDSVAQTILNYAAN
                     FEIELCCIGTSGERSAGHRGGLGLNTGRMINEAGFPILTCRSHPEKIHFRNILLPIDL
                     TRHTNEKVDRIIRFAMRFNSTVYLLAVSEFMEEFSFSRKSLLEKLESAALHIRSQGIK
                     CHTEIIRYDTVSRSVVEYASEIRADLLVVMSEPQSLVTELIFGNRTSKVIAQSPIPVI
                     SFRPVH"
     gene            9038..9910
                     /locus_tag="PK742_05590"
     CDS             9038..9910
                     /locus_tag="PK742_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HQU76097.1"
                     /translation="MEQVRASEMILNDDGSVYHLHLKPDHIADTIITVGDPDRVDSVS
                     RHFDRIEFKGVKREFVTHTGYIGQKRLTVLSTGIGPDNIDIVLNELDMLANMDLENRQ
                     VRKVHKTLTIIRLGTSGTICSDIPVHSLIVSDGAAGLDNLLHFYHAEKTEEETAMEQA
                     FNSWMDLPSGIVPYFFLADKELVQAFGSGFTSGITLTAPGFYGPQGRQVHAAIRIPDL
                     LDRAAGFRSGQTRITNIEMESAAIFGLCRALGHRAGSVNVILANRATGQFSVDPHRAV
                     EQMIIQALEVITRC"
     gene            complement(9911..>10158)
                     /locus_tag="PK742_05595"
     CDS             complement(9911..>10158)
                     /locus_tag="PK742_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76098.1"
                     /translation="FVFAAWITRKERDEELEKKLNDAFETGLQHLKEVAELVSVEGLS
                     AADLFTYYTRHISYRLDDRKKAGMELYLRHAAALPLL"
BASE COUNT         2668 a         2426 c         2418 g         2646 t
ORIGIN      
        1 ggtgctaaag cagccaaggc gctgggtatg atgggacgcc gctagatcag cctgcggtaa
       61 gcatccatag cagttccggc cagaacctcc ggtgtaaatc tttcccggta tgctattcct
      121 ttctggatgc agtttgctct gaactgatca tcctgaatcc tgaacatggc atctgcaagt
      181 gcatctgcat gttcagggtc aaagtacaaa gctgcattac caccggcttc cggaatggaa
      241 gaccgatcag aagcaagaac agcacaattg cataccaatg cctcagccac aggcatacca
      301 aatccttcat acaaggacgg gtagatcaat gctacggcct gatgatataa agcacggagg
      361 cccgcattat cagacgggat aaaatgcacc catttttcca gttttctttg cgtggtcagg
      421 aggcgcatag ccgatgcata ctttccgggg ccatgtaccg caactatcgg aatacgttgg
      481 gattcagatt gacgggacaa agcctccagt acgatctcaa aattctttct tcggttgccc
      541 agtcccacaa acagaaaaaa ctcagtgggt aaattttcag tgacggccga tggctcggct
      601 ggttcgtaga atatctgttc cacgccggga aacaacacca ccattcttcc cggatctgca
      661 ccataaagtt gttcgagatc attggccgtt gcctgactgg acgtaaagat gacatctgca
      721 tgcctgatag caaacctggt cttgtagtgg tagatgtttc tgtctatgat gccataggct
      781 tcaggatacc ggcgaaaaat aacatcgtgt acactcaccg cagaacgaca ttttgctgaa
      841 cgcatggtaa atggcagttc attgctaagt ccatgataga tatcgggctg ctctctggca
      901 atttgcctga tgatgctgaa actgcgccac agggccggtg tattggtgtc aggatagaga
      961 atacgtgaag atgccggctt aaaaggaaca cggccatttc gcggagtgaa caacagataa
     1021 tcgttgtccg gatagtattg ctccagtgag ttgatcaggt tacgaccgta atttcccaga
     1081 ccactggtat tgtgaaagat acgtttggcg tcaaaagcga tccgcatgga gcgaagatag
     1141 agagaagaaa aaatgcgaag cgggactttc gtcccgcttc attgttcatt actgaatgac
     1201 cactgttccc tgtaaacttt cggttccgtt ggtcagctgg tagatataga caccaggcag
     1261 atattcatta ttgctgatat taaaatgatc agactgagtt tctgtttcac tgaccatcct
     1321 tcctgaaaga tccagcatgc gcagctcaaa agtatcatta tgaggattgc tgaatcgcat
     1381 gacagataca cctgttaccg gatgcggctg gatagacact tgcagaccat aagcagcatg
     1441 ggcatcttcc agaccggtaa gggtgaaaca accattatct tttgctgtga tgatctcgtg
     1501 ataggcttca ttaacagcat tggttatata atccaggcag atcgttgtag gataggttgc
     1561 ctgaaaatag acccttgcca caggattagg tgtataattc ttttcataac cgatctcatc
     1621 atatccgata ccgatgatct catcattgct gaatgcaaaa cggaaatctg atgacatgat
     1681 ggattcagcc atcggcacca ccgaatcaat agtggtgata ccgagtatct tcagctggaa
     1741 ggcgagaatg tcggcagtag tattgaccga atagatgtcc aggtatcctt cacccgggtc
     1801 tatctcaccg atactatacg tcactgtatc gccatattca ttgaatccgt atggcaggtc
     1861 acacagctcg atcacgtggg tcacacccag attctcccag gcgcagccat tcacaagcgt
     1921 tgcatcatat acatctatat cactgtcatt gttggcgtcg ttacaagtgg cggccagaat
     1981 ggtgttatcc aggatctcag tggtgtactg cgttacatca tcgctggtct gggtgccatc
     2041 cagatcaaga tctcctatcc ttgccggccc gttgcagatg ccgttacaat cataggtagc
     2101 agaaccaaat atctcaccat agcagtcata atagggtgtg catccttcca ccctggtaac
     2161 ggtgggctcg ccacccggct catctctttc tatcaggata caaatggaag cccagttgtt
     2221 ggagagatct gtttccacat ggccgtcaat atccggatta ttctcccagt ttatgcgggc
     2281 cacgagggta tattgtccgt cgggtacatc cgtaaggtcc acccactggc agctggtacc
     2341 acgaccgtaa atatcataac accctgcgct gatacccata ttccatccgc cgtactgggg
     2401 tgtaccatca aagcaaccca catccatgac cgcataacca tttttatgtc cggatggtat
     2461 ttcattgccc agcgaatcat ataacaggta ttcaccatag tctgcataat gccagtgatt
     2521 gtggcaatcg ctgtacacaa acccgggtgc cccgtcagtg ggagacccaa ggtgataatc
     2581 gacatcgcca atgttcttga tcttcactcc gaacttgagc acataacgaa ttccggtgcc
     2641 tgttacacaa gcttcttcaa aagcacagtc atcccagtct gaatcatatg attccagcga
     2701 aaaatcagaa gtgaaggaag gatacccgtc aaaataagtt gaatcaagca ccaggtcagg
     2761 accggcaggg caatcaggag aacccggata gatacaggta ccgtcatcaa ctgtggccag
     2821 tggtgaataa ttacaggcgt atggatctgt acaaccgctt ggcgggcctg catacgtcag
     2881 gctccagttg atactggttc cttcgcagga ggatccaaga tctccaaccc tgatataata
     2941 ggtctctcct tccagcaaaa tgccggtgat ctctgcctgt tcgccgcagg catcatcatt
     3001 ataataaaca gttccttctg catattctga cagtaccagg ccatcacagt ggtcatagac
     3061 ccagagctgt gtattacagg tattatccag atcgcaggta gagatgatat aggtgccgta
     3121 ctctgctggt gtaaactcat accaggagtt gggtgccggt gctgtatagg ttccttctgt
     3181 cacttctata ctggaagagc aggctgtacc cggagggcaa ccgatatcga tggatgttcc
     3241 gtagtcataa tcgccggtaa aaaaagcaac cagctcatta tcataataga gttctactcc
     3301 atgaggagaa tagataccat cgccataact gtcatacagg tggaaggtat agcattctcc
     3361 atctactaca catgtactat cgctgttata atcgccgctg gcaatgatcc ccccatcggc
     3421 atcagtaata tcccagctga tctctccggg ccaggagtcc ggatcaatga aaatatgaac
     3481 cggcacttct gaggcaccgc aggtcaaggc gagcgcagga gtggtgcacc agacacctat
     3541 cgcaaatagt gataacaagg ctattcgggc agaggagtgc atacaggtaa ttttccatga
     3601 aaatacggaa aaccatcaaa aagcatgcag agcttcggca actgccaata tggttttcgg
     3661 ggcatttccc atgaagatcc ggttaccgat gatgaataca ggtcttttga ggaatgtata
     3721 ttcctgtagc agatacttct tataatctgc ttcggtcagg tccattttat tcaagcccat
     3781 acttcggtat ttcaaggcca ctttgctgaa cagtgattca taaggaggcg aagaatattg
     3841 ctgagcaacc agttctgcca tctcttcggc ctgtttccgg gttaccggct catatttgat
     3901 atcctgcaga acgaatggaa actgatctac ctgcagttct ttcataatgc gggcacaggt
     3961 atcgcaggtg gacaggtagt acagatgcaa tcgggttaca gcggccattt ctatctgctc
     4021 agttggaaaa tgaatgtagt tccttcttta tctgcagatt ctgctctgat ctgaccacca
     4081 tgattttcaa cgatcttttt gcatatggac aaccccagac cggtgccttt gtattcactt
     4141 gcggggtgta gcttggtaaa gaactgaaag atcttgtcgt gaaaatcatc gggaataccg
     4201 ataccattat cacatacact gaattcatag gtctggccgg catcttctac ctgcaccatg
     4261 accacagggt catcatctcc tctgaattta atggcattat ccaggaggtt ctggaaaaga
     4321 ctgaccagtt gttgttcatg cccgttgatc tcgggaaggt cttcgtacag gatgatggca
     4381 tcgctttctt ctacagacat cttgatatta tccagagccg tttccagaac cttgttgcag
     4441 gaaacttcag tcatctggtc agaggtatga tccagtctgg tataggtaag caggtccttg
     4501 accagatggt gcatacgttc tgcggcattc ttggcaaacc ccagatactc acgtccttct
     4561 gattccagac ctgcaccatg gttgcgcaga aagagggtaa tgtagttgct gatggttcgc
     4621 agaggttcct gcagatcatg tgatgccctg gaggcaaact gcttcaattc ctgattagat
     4681 tcttccagtt ggcgcagttt cagttccatt tgccccattt ctggcattgg ttctgcgatg
     4741 gagtgcagca gtatatcggt acctccaagg gtactgtaat ggcttctgac cgtacgccag
     4801 aaaagatcca tggtctttgc acccatcctg ggtttattcc tggaatatgc cagcacgcag
     4861 taagctgttc cgggtgcagg taagccaatt ccaatatgac cggtacttaa tggatcatgg
     4921 tataaccagg tacacggatg cacatcagat gttgcccatt cctgagatgg ccagcttccg
     4981 atggctacct tgaattcaag tggtacagca gattggccga atctgtcttc tgtggcaaac
     5041 aactgtttac ggatatacct ctccggagag ccgatctgct gcagcacaac cgcataatca
     5101 tatccgaaaa aagaaaccag cttttgtaat acaattgcct gtaatgcgtc agtatcctgc
     5161 tggcgataaa tatccagcag caactcttga agctgctccg ttgagggttg gttaattcgc
     5221 atgacaggtt acagctaaag tacaattatt tgtctgttct gtccaaatca taccaaacca
     5281 gcacttcacc ggcatgcttt cggcgaagat caggcacttt ggtgtcagga tgaggcttac
     5341 ctaccggaat cagcagaaag ggagactcat tagccggacg atccaggatc tgtttcagaa
     5401 aattcatagg acttggcgta tgcgtgagcg ccaccagtcc tgcctgatgt atggctgcca
     5461 gcaaaaaccc tgttgctaac ccaacagatt cagaaacgta ataattgttg tgtttgtgtc
     5521 catcagcatc tgtttcataa ttgcgtttaa aaaccacgat gagtgcagga actgtttcca
     5581 gaaattcctt gtgccagtca gttccaaatg gtttcaggtc atcaagccac tcctggggca
     5641 tgcgctgttc atatgatatt ttttcttctt tttcagcttc tatacggatc tgtctcttca
     5701 acttctgatc cgtaaccaca caaaaggtcc acggctgttt gttggcaccg gatggtgcgg
     5761 ttgatgctgt catgactatg ttctccaata cttcctgcaa aacaggttct gaagaaaaca
     5821 tccgcacact tctgcgttta ttcatccatt cataaaattg tctgcttctt tgcagcattt
     5881 cctggtgtgt gtatgtggga tgttgatagg atataaaagg aagatctttc atcgcaataa
     5941 ctttaagcgg tgaaaatacc cgaaagcctt caaacggtca acgctgcagc agaatctgta
     6001 tatcaggaaa gatccagcag gttcattgca ctggtgcgtc cttgcagcga tgatgccacg
     6061 gccagacagt ttcgagatgc cttgaaaaaa gagcatcata aagccgtgca tgtggtgctg
     6121 gcctatcgta tcgggcacac acaagctgtt gaatattcta ctgacgacgg agagcctacc
     6181 ggatcagcag gcaaaccagt tctgaatgaa ttgcgctcac gtatgctaag caatactgct
     6241 gtttgcgtag ttcgttatta cggcggcaaa aagctgggta ttcccggact gatccaggcc
     6301 taccgacagg ctgcttctca ggcgctggaa ctggcaggta gtcaagaact ggtccatcgt
     6361 atgcgcatca gggtgcatgt tcccgcttct gaacaacatc tggtagccaa cctgatatcc
     6421 ggaagcacgg caagaataga agacgccgtg tatgccgaaa ccactacttt tatcgtttca
     6481 ctggaacaca aggatgaaac ggttctgcaa aaactggagt ctcactggca actggctgtt
     6541 gaacgcattg ctgatacaga caattagttt gtcggctgcg gcacagcaaa ctacagaagc
     6601 tgaagcactt gcagctatcc gcttccctgg atattcattt gaaaccaatc atgtttttca
     6661 aacagatcat gctacctaca ccaggcttca gccgttttca gccggtattc aggtagatcg
     6721 tcaggtggtg atcgtacagc catacaacgg tgagatgagg gtaatgggat tgcttccacc
     6781 cgtgtcagat ctgatcacaa gcctggttta tacggaagct tgtatctgga tactgcaaaa
     6841 cgattcatgg gaagctgtac agatgagtac ccagacagac aggcatggtc acgacagaac
     6901 agatgttttc ctgaatgaag aaggcgatac catttcggtc agaagctgga gaagctattt
     6961 tggcgacagt accgttactg cacgtgtctt caggccagac ccgatcactg tggccgaggc
     7021 tgaatatggt ggcgatcttg ttgacaacaa tgatgcagat gacccagtgc tcaacagcct
     7081 gcgttcttta gtaaccgtga ccgccacctt tgaggatgat accttttttc tgtatgatac
     7141 cttcatcaga ctgaaagatt ttgctgcacc ggtacaacct gttgcctggt ctttgacacc
     7201 ggtctttgat gctggtcgcg gcgatcctgc ctttgaagat gtcaatacac tctaccacct
     7261 gcagcaatgc aggaactggc ttgtatcact gggcttcgaa gagctctgtt cttttgaaat
     7321 agacattgat acacatggag cagacggagc tgataattct tttttcagca gcggtggtac
     7381 gccttccata cagtttggcg aaggtggggt tgatgatgcg gaagatgcag atgtcattat
     7441 tcatgaatac atgcatgctc tgtctcacag cgctgcaccg ttcagcaata ccggaataga
     7501 aagacgagct atggatgaag gatatgcaga ctatctggca gcctcttaca gtctgcagtg
     7561 gagcagttac cggtggcagg atgtgttcag ctgggatggc cataacgaat tctgggatgg
     7621 ccgaaccact gataatgaca agcactaccc ggaagataat tctttaaata tctatgccgc
     7681 cagtggtatc tggagcggcg ccctggctga tatcctggaa agcataggga aagaaaatac
     7741 agacaaactt gtgctggaag cactgtacgg cagttatgat gaaatgacca tgccagaagc
     7801 tgcactctgg ttgctggacg tggaggatat gctgtttggc gggttgtacc atgaggaggt
     7861 atttacaata ctggacacac ggggtctgtt gccgtcaaca ggcgttcagg aacaacaatt
     7921 atataatatt tcattttata atacaaatgc ctttgcggat ggaaccggtc cattgcttat
     7981 catcactgac cctggtaaac aacttcttca gatgtttgac atgtcaggcc gcatggtatc
     8041 atcattttgc ggagaaggcc cggagttgga gtggatgcca ccacttacct caggcagcta
     8101 tctgttaaca gttacaggcc ggaccacagg tagcacgatg ctgatcgtga agtaatcaat
     8161 gtacaggacg gaaactgata accggtatag gcgattgtgc gatcacttta ctggttcggt
     8221 taccaaatat cagttcggta accaggctct gaggttcaga catgaccacg agcaggtctg
     8281 cacgtatctc tgatgcatac tccacaacag aacgacttac cgtatcatag cgaatgatct
     8341 ccgtgtggca tttgataccc tgtgaacgaa tatgcagcgc agcagactcc agtttctcca
     8401 acagtgactt tctgctgaaa ctgaattctt ccatgaactc tgaaacagcc aacaaataga
     8461 ctgtactgtt gaaacgcata gcaaagcgga tgatacgatc cactttttca tttgtgtgcc
     8521 tggtcaggtc tataggcaac agtatattcc tgaaatggat cttttccggg tgactgcgac
     8581 aggtgaggat aggaaaccct gcttcattga tcattcttcc tgtgttcaga cctaagccac
     8641 cacggtgtcc tgctgatctt tcgccgctgg tgcctataca gcacaactct atttcaaagt
     8701 ttgcggcgta gttcaggatc gtctgtgcca ccgagtcttc agacaattta tagtctgcat
     8761 ggatcctgaa ggctccgcca ctccggttcc tgaatgttcg caactgctgc ctgatatcct
     8821 gcaagagtgc acggtaggta cgcacatcac ccccctgttt cctaagcatc tccctgttgg
     8881 ccacatggat cagatgaata ccactgtctg tgcggttggc atagaaaacc gcatattcaa
     8941 gtgctgcaaa tgactcttca gaaaaatcag taggtaccag tacctgcttg gcatagcggt
     9001 tcatcagccg ttaaagttac gcagtatctt tggttgcatg gaacaagtgc gagcaagtga
     9061 gatgatcctg aatgacgatg gttctgtata tcacctgcat ctgaaaccag accatatagc
     9121 agacaccatc attaccgtgg gtgatcccga cagggtagat agtgtaagca ggcattttga
     9181 ccgcattgaa ttcaaaggcg ttaagagaga attcgttacg catacaggct acatcggaca
     9241 aaaacgtctg actgttctgt caacaggcat agggccagac aacatagata tcgtactgaa
     9301 tgaactggac atgctggcca acatggatct ggagaacagg caggtcagga aggtacataa
     9361 aacgctcacc atcattaggc tgggcacatc aggtaccatt tgcagcgaca ttccggtaca
     9421 ttctctgatc gtttctgacg gagcagcagg tctggataat ctgctccact tttaccatgc
     9481 agaaaaaacg gaagaagaga ctgccatgga gcaggcattc aacagctgga tggaccttcc
     9541 ttcaggtatt gttccctatt tttttctggc cgataaagaa ttggtgcagg catttggctc
     9601 cggttttaca tccggtatta ccctgaccgc acccggtttt tacggacctc agggaagaca
     9661 ggtacatgca gccattcgca ttcccgatct gcttgacaga gcggcaggtt ttcgatctgg
     9721 ccagacacgt atcaccaata ttgaaatgga aagtgctgcc atattcggtc tgtgcagggc
     9781 attgggtcac cgtgcaggat cggtaaatgt cattcttgcc aaccgggcca cagggcagtt
     9841 ttcggttgat ccgcatcggg cggtggaaca aatgatcatc caggctctgg aagtgatcac
     9901 ccgttgctga tcaaagcaat ggcagtgcag cggcgtgacg cagatacagc tccataccgg
     9961 ccttttttct atcatccaga cgataactga tatgcctggt gtagtaggtg aacagatcgg
    10021 cagcagacaa tccttctacg ctgaccagtt cagcaacttc cttcagatgc tgcaaaccgg
    10081 tttcaaaagc gtcattcagt tttttttcca gttcttcatc tcgttccttc ctcgtgatcc
    10141 aggcagcaaa aacaaaat
//
LOCUS       DAOMAX010000074        10092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_907_length_10092_cov_4.617515, whole genome shotgun sequence.
ACCESSION   DAOMAX010000074 DAOMAX010000000
VERSION     DAOMAX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_907_length_10092_cov_4.617515"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PK742_05600"
     CDS             <1..975
                     /locus_tag="PK742_05600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU76099.1"
                     /translation="IHPAYAVEHGMEIHPLSKRIWKDIRADQGAGPFRLVRRVLKRGL
                     QLSLEYPDIQFLFTTRRLLRKKNGYDLLITIAVPHAIHWGAALARTRKHRIAKTWVAD
                     CGDPFMFEKTDTFRKWFYFAWFEKLFCRKADHITVPIEDAKEAYYPEFRHKIKMIPQG
                     YDFNEVPIDHNLYRKNPVPTFAYAGAFIPGVRDPKNFLEYLLTLKCPFLFIIYTNQTS
                     IVIPYAERSEGRIEIRPYVPRAELLPVLAGMDFLVNIGNGTNIQSPSKLIDYQICGRP
                     VLSVDSFSLNSSVITQFLSGDYSGQISLGDLSAYDIKNVSKQFLQLTQ"
     gene            1115..2260
                     /locus_tag="PK742_05605"
     CDS             1115..2260
                     /locus_tag="PK742_05605"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HQU76100.1"
                     /translation="MRSSWAKNIVWLFVIFFGLTFVISNPKIDANRYNDKLQEMGDHD
                     YTFQTLGHQLYRDTEYVDVLQPLLTFVVSRFTSDYRVLFAAFGLIFGYFYSRNIWYLI
                     DRTSGRFKWYGVTLLIIFTVINGFWNINGFRFWTAAHIFVFGVLHYLETGKAKHLFLT
                     ILTVFVHFSFFFPVLVFLLYLAGGNRKHLYFYFFLLTFTFSEASLTQVREYLSFLPSI
                     FEAKVSNYTSDVYEQVLNERKSLLNWYVQYYGAALRYVVLVWLAWIYFQGGAFIREHA
                     LAGKLFSFVLLLYALSNLGSLIPSGNRFIVLSNLFAVALIFWYYHSNRQPGWINRIAV
                     LSMPALALYFLISVRIGLDMMGVQMLVGNPVTAIFFRDETPLVDWIR"
     gene            2266..3228
                     /locus_tag="PK742_05610"
     CDS             2266..3228
                     /locus_tag="PK742_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76101.1"
                     /translation="MLRRINRHLQDLNQTRLDRKICRQLRSAGREWNAEHVRVIFSES
                     RGGSTWLFEMLLQSGEMVSQWEPLHPVRGTVSELPLHYGDRPYLAPGEKDDALLNCLI
                     ASFSGKAWTPWTARYNSPESVDSADKLLVKFVRANMLAPWILHQIQLRYKPVMLIRHP
                     VAVALSQLDAFRGADRDAVTYKVPNCHNNQRFVQNEQYLNGLEGALERKVALWCINNL
                     PVLQNEQFMLQVQPVFYEYLVLNPQEQFAQIALNWDLTFSGVQANNASSTTGRAGVDQ
                     EPLAQISKWMGRINDQQKENCKNILQYFGCTLYSVDDPLPLNIK"
     gene            3231..4298
                     /locus_tag="PK742_05615"
     CDS             3231..4298
                     /locus_tag="PK742_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76102.1"
                     /translation="MKIADLFNVLSYRSSDKICVFLVDDYGHLGMSGELTEKEINTCP
                     GILPGDVFNRYDGLESPDDLHSLFDALTDYKGSDNKPVVFTPVVCTGNPTFSDEENDM
                     YSWESFNSSLKRSYGKEILDLWQQGENEGVFQPELHGREHIHPRILINKLKEQAPAFT
                     WMRDHRSIAVLNDGNVHLRPFKWMESQDEEWQQKMLGDAVGIFESFFKRKPALFVPPG
                     LIHGKHTEQLAGEYGILYMDSARSAMRSEANGQMKREIAWTGKRSNEIRYLCRNAMFE
                     PATFGENSITQTLQAADKAFRWRVPLVISSHRVNFAGGRDESNRARGLLILRKLLEIL
                     TRRYPDMRFISASELISLMTI"
     gene            4385..5500
                     /locus_tag="PK742_05620"
     CDS             4385..5500
                     /locus_tag="PK742_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76103.1"
                     /translation="MKNQLRDLVLGRLTTRHKRRILIIESDDWGTIRMPSSSVYQMLL
                     SKGYPVDKCPYNRNDSLETDADMLALIEVLRSAKDHTGRPAVITANWIAANPDFDRIR
                     DSGFQEYFWEPSNSTLERHPDSRQVFALQKQALTERLFYPQFHGREHVHVAAWMKKLR
                     AGDEAIHSAFNHRMISFNVNEVPSCESDIMDAYNAGDTNDLKFSEASVTEGLKLFQET
                     WGFASTTAIPPCYFWHEAHEQVMYNNGVMGIQGIAVQKSPVVGKEKWKKKYHWTGQRN
                     KNGQIYLRRNVFFEPSILGREGAVSSAFAGIARAFEKGHAAIISSHRLNYIGTINPEN
                     RTSNLKLLKELLQLVVSKFPEVEFMHSADYLEFIRKP"
     gene            5536..6624
                     /locus_tag="PK742_05625"
     CDS             5536..6624
                     /locus_tag="PK742_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU76104.1"
                     /translation="MLYNIDPGGLEVYLLRFFRHYQGQFRATLICKSGKTGALLKGYE
                     DAGVEVITVRTGYMNPVSLWKIYRLLRNRKFDGICDLTSNFGGLYMWLARKASIRSRV
                     AYYGQSTNHFRSSALKSSFDRMVNRLVYRNATAIVLNSHTATDNFFPYRVAGDPRFKV
                     VYNGVDAAMFSDEKDDEMKQALGIPVSSFVIGHTGRFDEKKNHQAVLDLAVRACATWD
                     DCYVICCGKGTEQLQESINRRQLDGRVLALGYRDDVHRILRTFDVFYFPSYTEGQPNS
                     LIEAMMAGLPFVASDIVPIRDTVPEHLQGQLVGPDDVGSAFHLIQRMKEDRQLRIRLS
                     CRDWAIEAYNANDRFREFLESVAGDQIV"
     gene            6621..7787
                     /locus_tag="PK742_05630"
     CDS             6621..7787
                     /locus_tag="PK742_05630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU76105.1"
                     /translation="MKVLFAYNGRVEIDEHGRYYGNELNDRLVERYRYFGDQVTFIVR
                     TRPIHAVAASELQPFESNLQVIPVPEMNTPFLFLRNRTVIHHKIKHAVNEHDVIIVRE
                     PGYVGRIAAQYARYFNKPYLTEVVGDPYDALSNHSTLGKIYAPIARRSMLKSIAKADP
                     VLYVTKEYLQQRYPSTGYQLGLSDVVLKPSGEEVLESRIHKIGMRKHDDELVVGTAAA
                     LDVKYKGHEYVLQAMSILARQGIQLKYQVAGKGTGDRLVRLANDLGVRSQLDIIGQIP
                     HGQVFDFLDNIDLYIQPSVTEGLPRALVEAMSRACPCLGSRVGGIPELLPDKYLFEKR
                     NAKQLAGIIAGLSRHDLENMARQNAEAAKRFDPERSAESRLEFYDRFKRTYSSQ"
     gene            7784..8455
                     /locus_tag="PK742_05635"
     CDS             7784..8455
                     /locus_tag="PK742_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQU76106.1"
                     /translation="MNLSRIKIGLYEQAERLVGKQAIDRMYLDRCIKIYRKQQVIFIH
                     IPKAAGTSVARAVLGKRAGHFTAETVRDRMGNDPYYKLYSFAVTRHPVDRLYSAYRYV
                     KGNGGTEGGVRRHPDYDGPLFHTFEKFVMEWLPLQNILNGPVIFRPQYSFLFDSGIEL
                     LVNDVLKLEEPEKLEQILTERLGRKIILERRNMSVTDLDNGISSLVRRRISEIYHRDH
                     ELLGY"
     gene            8465..9607
                     /locus_tag="PK742_05640"
     CDS             8465..9607
                     /locus_tag="PK742_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQU76107.1"
                     /translation="MKGKILYTATSDIHLAAFHYPYIKWLDEQGYEVHLAMEKRADLE
                     FSHARQIHYMPFPRTPLDRRNVSTYRQLKNLIDKEQYDLIHCHTPMPGVLTRLAARAR
                     RKKGAAVLYTAHGFHFFKGAPLKYWLSYFPAEYALSFITDAIITINSEDFTYADKRLH
                     PRKAFMIPGIGIDSGKFRVYSEEEKKAVRLKYGFKQQQFLLLYIAEFIPRKNHRFILE
                     NLPALIRQVPTVQVIFAGRGELMDQMLNLARNLRVEDHVRFMGFMHDLGPLAAIADVG
                     ISASRQEGLGLGLAEQMLCGVPVVATEDRGHKELVIHEQTGLMFPQGDQIAFREALVR
                     FSKEPSLRQKMGNAARLHAEKFRIEASLDSMRNIYNGYLKPDQKTD"
     gene            9607..>10092
                     /locus_tag="PK742_05645"
     CDS             9607..>10092
                     /locus_tag="PK742_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76108.1"
                     /translation="MLKGTKNKWIYLAYYFRQLDQDKMRRFVRFASEKTGRSRWSLWA
                     DAIRSVFRYNTGLIDYFLFRFYEKTPEERAAWAGTGFMYEYHLAMNPLDARDILANKI
                     RFYEKYDQFVVHAHCTIDDLHANNEQAVKVLRHPTDKLVLKDALGQCGWGIEVISKKE
                     FS"
BASE COUNT         2819 a         2080 c         2491 g         2702 t
ORIGIN      
        1 attcatcctg catatgctgt tgaacacggc atggagatac atccgctatc aaaaaggatc
       61 tggaaagata tccgtgcaga ccagggagca ggcccgttca gattggtacg gcgagtactg
      121 aaacgaggcc ttcagttatc actggaatat cctgacatac aattcctgtt taccaccaga
      181 agattattgc gaaagaagaa tggatatgac ctgctgatca caatagctgt gccgcatgcg
      241 atccattggg gggctgcatt ggccaggaca agaaagcatc ggatagcaaa gacctgggtg
      301 gcagattgtg gcgatccatt catgttcgag aagaccgata ctttccggaa atggttctat
      361 tttgcctggt ttgaaaaact tttttgcaga aaggcagatc acatcacagt gccgatcgaa
      421 gatgcgaaag aagcgtatta tccggaattc cgccataaga tcaaaatgat cccgcaggga
      481 tacgacttta atgaagtgcc tattgatcat aacctctata ggaagaaccc tgtccccaca
      541 ttcgcatatg ccggagcttt tattccgggt gtgcgggatc cgaagaactt cctggaatat
      601 ttgctgacac tgaagtgtcc attcctattt attatttata ctaatcaaac gagtatagtt
      661 attccatatg ctgaacgatc agaaggcaga atagagataa ggccatatgt gccaagagct
      721 gaattactgc cggtactggc tggtatggat tttctcgtga atattggtaa tggtacaaat
      781 attcaatctc ccagcaagct gatcgattat cagatatgtg gaagacctgt tctgtctgtt
      841 gattcctttt cgctgaattc aagtgtcata actcagtttt tgtcaggtga ttattcagga
      901 cagattagcc tgggtgatct gtcggcctat gacattaaaa atgtttcaaa gcaattttta
      961 caacttaccc aatgatgaac gatccggaat gcagccccaa accttaagaa gcagaagcag
     1021 ggtccaggat gaggacagaa gaagaggagg ctcatttgcc agggtgattt ttctggtctg
     1081 gcctttcggt tctcttgcct ttaccgcgta tcatttgcga agttcatggg ctaagaatat
     1141 tgtctggctt ttcgtgatct tctttggttt gaccttcgtt atctccaatc ctaagatcga
     1201 tgccaatcga tacaatgata agttgcagga aatgggggat catgattata catttcagac
     1261 ccttggccat cagttgtacc gcgatactga gtatgtggat gtgctgcagc ctttactgac
     1321 atttgtggtt tccaggttta ccagtgatta tcgggttctc tttgctgcat tcgggctcat
     1381 ttttggatat ttctattccc ggaatatctg gtacctgatc gaccggacca gtggtaggtt
     1441 caaatggtat ggcgtaacac tgctcattat tttcacggtc atcaacgggt tctggaatat
     1501 taatggcttc agattctgga ccgctgctca tattttcgta ttcggtgtgc ttcactattt
     1561 ggagacagga aaagcaaagc atctattcct gaccatttta actgtgtttg tgcatttttc
     1621 ttttttcttc cctgttctgg tctttctgct gtatctggcg ggaggaaaca gaaagcatct
     1681 atatttctac tttttcctac tgacctttac tttttcagag gcgagtctta cccaggttag
     1741 agaatattta tccttcctgc catccatttt tgaagctaaa gtgagtaact ataccagcga
     1801 tgtatatgag caggtattga atgaaagaaa gagtttgctg aactggtacg ttcagtacta
     1861 tggagccgca ttgagatatg ttgtactcgt atggcttgcc tggatatatt tccagggagg
     1921 ggcatttatc agggaacatg cacttgcagg taaactgttc tcttttgtat tactgttata
     1981 tgcattatct aacctgggca gtctgatacc aagcggaaac agatttattg ttttgagtaa
     2041 cctgtttgca gttgccttga tcttttggta ttaccattcc aaccgtcagc ctggatggat
     2101 caacaggatc gctgtgttaa gcatgccggc actggcgctt tatttcctca tatccgtgag
     2161 aattgggctg gatatgatgg gtgtgcaaat gctggttggt aaccctgtta cggcgatctt
     2221 tttcagagat gaaacgccat tggttgattg gataagatag atgtaatgtt acgcaggatc
     2281 aacagacatt tacaggatct gaatcagaca agactggatc gtaagatctg cagacaactc
     2341 agatctgccg ggcgggaatg gaatgctgag catgtcaggg tcatttttag tgaaagcaga
     2401 ggcggaagca catggctgtt tgaaatgttg cttcagtcag gagagatggt gtctcaatgg
     2461 gaaccactcc atcctgtcag aggtacggtc agtgaacttc ctctgcacta tggcgacaga
     2521 ccatatcttg caccgggcga gaaagacgac gctttactga attgcctgat cgcttctttt
     2581 tccggaaagg catggacccc ctggaccgcc aggtacaaca gtcctgaatc agtagattcg
     2641 gcagacaaac tcctggtaaa atttgttcgt gccaacatgc ttgctccctg gatcctgcac
     2701 caaattcaat taagatacaa gccggtcatg ctgatcagac atcctgtcgc tgtggcatta
     2761 tctcagttag atgccttcag aggggctgat cgggatgctg ttacttataa agtaccgaac
     2821 tgtcataaca accagcgatt tgtgcaaaat gagcagtatc tgaatggtct ggaaggtgca
     2881 ctggaacgaa aagtagcact ctggtgcatc aataatctgc cagttcttca gaacgaacaa
     2941 ttcatgttgc aggttcagcc ggtattttat gaatatttgg tgttgaaccc gcaggagcag
     3001 tttgcacaaa tagctttgaa ctgggatctg acattcagcg gtgtgcaggc caataacgca
     3061 agctccacaa ccggaagagc cggagttgat caggaaccat tggcacagat atcaaaatgg
     3121 atgggcagga taaatgatca gcagaaagaa aattgcaaaa acatcctgca gtattttggg
     3181 tgtacgctgt attctgttga tgatccactg ccactcaaca tcaaataggt atgaagatcg
     3241 ccgatctgtt taatgtcctt tcttaccgtt cttctgataa gatctgtgtc ttcctggtgg
     3301 atgattacgg tcacctggga atgtccggcg agttgacaga aaaagagatc aatacctgcc
     3361 ctggtatttt accgggtgat gttttcaaca ggtatgatgg cctggagagc cccgatgacc
     3421 ttcattcttt atttgatgca ctgaccgact acaaaggctc tgacaacaag cccgtagttt
     3481 ttactccggt tgtctgcacg gggaacccaa cattcagtga tgaggagaat gatatgtata
     3541 gttgggaatc cttcaattca tctctgaaga ggtcatatgg aaaggagatc ctggatcttt
     3601 ggcaacaggg agagaatgaa ggagtttttc agccggaatt gcatggaaga gagcatatcc
     3661 acccccgtat tttgatcaat aagctgaagg aacaggctcc ggcttttacc tggatgagag
     3721 atcaccggtc gatagctgtt ttgaatgatg gaaatgttca tttgaggcca tttaaatgga
     3781 tggaaagcca ggatgaagaa tggcagcaaa aaatgttggg agatgctgtc ggaatatttg
     3841 aaagcttttt taagaggaaa ccggctctgt tcgttccgcc gggtttgata cacggaaagc
     3901 atacagaaca actggctggt gaatacggga tactgtatat ggattcagcc agatcagcaa
     3961 tgcgctccga agcaaatgga caaatgaaaa gagagatcgc atggaccggg aaaagatcca
     4021 atgagatcag atacctgtgc cgcaatgcta tgtttgaacc tgccacattc ggcgaaaatt
     4081 ctataaccca aacacttcag gctgctgata aagctttcag atggcgggtt cccctcgtta
     4141 ttagttctca tcgggtgaat tttgccggag gcagagatga atccaacaga gcaagagggc
     4201 ttttgattct aagaaagctt ttagaaattc tcaccagaag gtatccggat atgcgtttta
     4261 tatctgccag tgaactcatc agtctaatga caatttaggt attagatgtt caggcggcat
     4321 tttgttctgg aacccggatc tgaaaaacac gtactacgtt taaacaacac cttgtaataa
     4381 gtcagtgaag aatcaattac gtgatttggt tctgggtcgt ctcaccacaa gacacaaacg
     4441 ccgcatcctc atcatcgaat cagatgactg gggtaccatc cgcatgccct catcttcggt
     4501 ttaccagatg ttgctgtcta agggatatcc cgttgataaa tgcccctata accgcaacga
     4561 ctctctggaa acagatgctg atatgctggc actgatagaa gtactgagat ctgcaaagga
     4621 ccatacagga aggccggctg tcatcactgc taactggatt gccgccaatc ctgattttga
     4681 tcgcatcagg gactctggtt ttcaggaata tttctgggaa ccgagcaaca gtacactgga
     4741 acgccatcct gatagcagac aagtctttgc attgcaaaag caggcgctga ctgaaagatt
     4801 attctaccct cagtttcatg gtcgtgaaca cgtacatgta gccgcctgga tgaagaaact
     4861 gagggcaggt gatgaagcca tacattcggc tttcaaccac cgcatgatct cattcaatgt
     4921 aaacgaagtt ccatcctgtg aatcagatat catggatgcc tataatgcag gagatactaa
     4981 tgatctgaag ttttcagagg cttctgttac agaggggctt aagctgtttc aggaaacctg
     5041 gggttttgct tccaccaccg ctatacctcc atgttatttt tggcatgaag ctcatgaaca
     5101 ggtcatgtac aacaatggtg tgatgggcat tcagggcatt gctgtacaga aatcgccggt
     5161 ggttggcaaa gagaaatgga agaagaaata ccattggacc ggtcaaagga ataagaacgg
     5221 tcagatctac ctgagaagga atgtgttctt tgagccgagt attcttggca gagaaggggc
     5281 cgtttccagt gcctttgcag gcattgcaag ggctttcgaa aaagggcatg ctgccatcat
     5341 cagttctcac aggcttaatt atataggcac cattaacccc gagaacagaa ccagtaacct
     5401 gaaattacta aaggaactac tgcaactggt cgtatcaaaa tttcctgaag tggaattcat
     5461 gcattctgca gattatctgg aatttattcg caaaccatga ccaccgaatc tcgagtgaaa
     5521 aagaacttct gttttttgtt gtacaacatc gacccgggtg gcctggaagt gtatctgctc
     5581 cggtttttca gacactacca ggggcagttc cgagctaccc tgatatgtaa gagcggtaag
     5641 acaggtgcct tgctgaaagg gtatgaggat gcaggggttg aagtgatcac ggtcaggacg
     5701 ggctatatga atcctgtctc actgtggaag atctatcgcc tgttgcgaaa cagaaagttt
     5761 gacggcatct gtgacctgac cagcaacttt ggcggactgt acatgtggct tgcccgaaaa
     5821 gcatccatcc ggtcacgagt agcctactat ggtcagtcca ccaatcactt caggtcttct
     5881 gctctgaaat catcgttcga cagaatggtc aacagactgg tgtacaggaa cgccactgcc
     5941 attgtgctga attcgcatac agctaccgat aatttttttc cgtacagagt agcaggtgat
     6001 cctcgcttca aagtggtgta taacggtgtg gatgctgcca tgttcagcga tgaaaaggat
     6061 gatgaaatga aacaggcatt gggcatcccg gtctcttcat ttgtgatcgg acataccgga
     6121 agatttgacg agaagaaaaa tcatcaggcg gtcctggatc tggcagtacg tgcctgtgca
     6181 acctgggatg attgctatgt gatatgctgt ggtaaaggaa cagaacagtt gcaggaatcc
     6241 atcaaccgaa ggcaacttga cggaagggta ttggcattgg gctatcgtga tgatgtgcac
     6301 cgtattctcc gcacatttga tgtgttttat ttcccatcat atacagaggg gcaacccaat
     6361 tcactgatcg aagcgatgat ggcaggactt cctttcgtag catctgatat cgtaccgatc
     6421 agagacacag tacctgaaca tttgcaaggt cagctggtag gccccgatga tgtgggatct
     6481 gcctttcatt tgatacagcg aatgaaagaa gatcggcagt tacgaatcag attgtcctgc
     6541 agagactggg caattgaagc ctataatgcc aatgacagat tcagagaatt tctggaatct
     6601 gtagcaggag atcagatagt atgaaagtgc tatttgcata taacggcagg gtggagattg
     6661 atgaacatgg ccggtattat ggcaatgagt tgaatgatcg tttggttgaa cgataccgtt
     6721 attttggaga ccaggttacc ttcatagttc gtacaaggcc cattcatgct gttgctgcat
     6781 ctgagttgca gccttttgaa agtaatctgc aggtcattcc tgttcctgaa atgaacacgc
     6841 cattcctgtt tttacgaaac cgcacagtaa tacatcataa gatcaaacat gcggtaaatg
     6901 aacatgacgt gatcattgta agagaaccgg gatatgtagg cagaattgcg gcacaatacg
     6961 cacgatattt taataaacct tacctgactg aggtagttgg cgatccatac gatgctttga
     7021 gcaaccacag tactttagga aagatctatg cccccattgc cagaaggtct atgttgaaaa
     7081 gcattgccaa agctgatcct gttttgtatg ttaccaaaga gtatctgcag cagagatatc
     7141 caagcactgg ttatcagtta ggattatcag atgtagtact gaaaccttcc ggtgaggaag
     7201 tattagagtc ccggatacac aaaataggca tgcgcaagca tgatgatgaa ctggtagtag
     7261 gaacagcagc agcactggat gtgaagtata aaggacatga atatgtattg caggcaatga
     7321 gtattcttgc caggcagggc attcaactta agtatcaggt agcaggaaag ggaaccggcg
     7381 atcgtttggt gagattggcg aacgatttgg gagtaagatc tcagctggat attattggac
     7441 agattcctca cggccaggtc tttgattttc tggacaacat tgatctgtat attcaaccga
     7501 gtgtaacaga gggccttcca agggcgttgg ttgaagcaat gagcagagcg tgtccttgtc
     7561 tcggaagcag ggttgggggc atacctgaat tactgcctga taagtacctt tttgagaaaa
     7621 ggaatgcgaa acaactggct ggtatcatcg ccggactgag cagacatgat ctggaaaaca
     7681 tggccagaca aaatgcagaa gcagccaaga ggtttgaccc ggaaagatcc gcagaatcta
     7741 gattggaatt ctatgaccgt ttcaaaagaa cctattcatc acagtgaacc tctcccgcat
     7801 caaaatagga ttatatgaac aggccgaaag gcttgttggt aaacaagcta tagacaggat
     7861 gtacctggat cgctgtatta agatctatag aaagcaacag gtcatattca tacatatacc
     7921 aaaagctgca ggtaccagcg tggccagagc tgtgctgggc aaaagagcag gacatttcac
     7981 tgcagaaact gtccgggaca gaatgggtaa tgatccatac tataagttgt actcttttgc
     8041 agttaccaga catccggttg acaggctcta ttctgcatat cgctatgtga agggaaacgg
     8101 tggaacagaa gggggcgttc gcagacatcc ggattatgac ggacccttat tccatacatt
     8161 tgagaaattt gtcatggaat ggttgccgtt gcaaaacatc cttaacggcc ctgtgatttt
     8221 caggccgcag tacagttttc tctttgattc aggcatagaa ctgctggtga atgatgtact
     8281 gaaactggag gaaccggaaa agctagagca gatcctgacg gaaagactgg gaagaaaaat
     8341 aatattggag agaaggaaca tgtctgtaac tgatctggat aacggcattt catccttggt
     8401 gcggagaaga atatctgaaa tatatcaccg tgatcatgaa ttattgggat attgaatgtt
     8461 gcaggtgaaa ggtaaaattc tgtacacagc caccagcgat atacacctgg ccgctttcca
     8521 ttatccatac ataaaatggc tggatgagca gggttatgaa gtgcatctgg cgatggagaa
     8581 acgggcggat cttgaattca gtcatgccag gcagatacat tatatgccat tcccgcgaac
     8641 acctcttgac cgtcgtaatg tatccaccta caggcagttg aagaacctga tcgataaaga
     8701 acaatacgac ctgatacatt gccatacacc tatgccggga gtactcacaa ggctggcggc
     8761 cagagccaga aggaaaaagg gggctgcggt cttgtacact gcccacggct ttcatttttt
     8821 taaaggggct ccattgaagt actggctttc ctatttccct gcagaatatg ccttgtcatt
     8881 tatcactgat gccatcatca ccattaacag tgaagatttc acctatgctg ataaaagact
     8941 gcacccccga aaggcattta tgataccagg aattgggatc gactctggaa aattcagagt
     9001 gtattcagaa gaggaaaaga aagctgtcag gcttaagtat ggttttaagc aacagcagtt
     9061 tctcctgctg tatattgctg agttcatccc ccgaaagaat catcgattca ttctggaaaa
     9121 tctgccggct ttgatcagac aggtcccgac cgtgcaggtg atattcgccg gtcgcggtga
     9181 gctgatggat cagatgctga atcttgccag aaaccttcgg gtggaagatc atgttcgctt
     9241 catgggcttc atgcatgacc tgggaccgct cgcggcgatt gctgatgtag gtatctcggc
     9301 aagcaggcag gaaggacttg gtctgggact tgccgaacaa atgctgtgtg gagttcctgt
     9361 tgtggcaact gaagacagag ggcacaaaga actggtcatc catgaacaga caggtttgat
     9421 gtttcctcaa ggcgatcaga tcgcctttcg tgaagcactt gtaaggttct ctaaagagcc
     9481 ttcattgcgc caaaaaatgg gaaatgccgc ccgtttgcat gccgaaaaat tcaggatcga
     9541 agcatcgctt gatagcatgc ggaatattta taacgggtat ctgaaaccgg atcaaaaaac
     9601 ggactgatgc tgaagggaac aaagaataaa tggatctatc tggcctatta cttccgccag
     9661 ttggatcagg acaaaatgcg aaggtttgtt cgttttgcct ctgaaaagac aggcaggtcc
     9721 cgctggtctc tctgggctga tgccatacgt tccgtgttca gatataatac cggcctgata
     9781 gattatttcc tgttcagatt ttatgagaag actcctgaag aaagagctgc atgggcagga
     9841 accggattca tgtatgaata ccatcttgct atgaatcctt tggatgcaag ggatatcctg
     9901 gccaataaga tcaggtttta cgaaaagtac gatcaattcg tggtgcatgc acattgtacg
     9961 attgatgatc ttcatgccaa taatgaacag gctgtcaagg tcctccggca tccgacagat
    10021 aaactggtac tgaaagatgc cctcgggcag tgcggctggg gcattgaagt gatcagcaaa
    10081 aaagagtttt ct
//
LOCUS       DAOMAX010000075        10068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_909_length_10068_cov_4.277839, whole genome shotgun sequence.
ACCESSION   DAOMAX010000075 DAOMAX010000000
VERSION     DAOMAX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_909_length_10068_cov_4.277839"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="accB"
                     /locus_tag="PK742_05650"
     CDS             <1..328
                     /gene="accB"
                     /locus_tag="PK742_05650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQU76109.1"
                     /translation="VAPVAPPPVQASENKPVVKESDTTTENGNLITIKSPIVGTFYRS
                     PGPDKEVFVKVGDDIEVGSVLCIIEAMKLFNEIESEFAGKIVKILVDDASPVEFEQPL
                     FLIEPR"
     gene            356..1705
                     /gene="accC"
                     /locus_tag="PK742_05655"
     CDS             356..1705
                     /gene="accC"
                     /locus_tag="PK742_05655"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQU76110.1"
                     /translation="MFKKILIANRGEIALRVIRTAREMGIKTVAVYSTADKDSLPVRF
                     ADEAVCIGPAPSRESYLSIPNIMAAAEITNADAIHPGYGFLSENEMFADICQKYGIKF
                     IGPRADHIRSMGDKITAKESMRKAGVPVVPGSDGLLKDVADAREQAKKIGYPVMLKAT
                     AGGGGKGMRIVWGEKDLENAYASASTEAKASFNNDGLYMEKFIEEPRHIEIQVAGDQY
                     GKACHLSERDCSIQRRHQKLVEESPSPFVDEKLREKMGEIAVRAAESIGYEGVGTIEF
                     LTDKHKNFYFMEMNTRIQVEHPVTEEVVDYDLIKEQIKLAAGEPISGKNYYPILHAME
                     CRINAEDPSQGFRPSPGKIVNFHSPKGHGVRVDTAVYAGYVIPPYYDSMIAKLICRAR
                     TRDEVILKMQRALDEFIVEGVKTTVPFHKKLMRDERFRAGVFDTSFMNSFDLTDLPS"
     gene            complement(1709..2644)
                     /locus_tag="PK742_05660"
     CDS             complement(1709..2644)
                     /locus_tag="PK742_05660"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQU76111.1"
                     /translation="MPVAETHYQLPGQTGFYRGKVRDVYSIGEDLLVMVASDRISAFD
                     VVLPRPIPYKGQILNQIAAGFLKQVQHIVPVWLLQTPDPRVAIGLRCQPYKVEMVIRG
                     YLTGHAWREYSSGKRTICGVQVPDGLREHDPFPEPIITPTTKASEGHDEDISKEEIIA
                     RGIVPEAEYLQLETITRQLFAFGTRYAAAQGLILVDTKYEFGVHNGNIMLMDEIHTPD
                     SSRYFYAAGYEDRQAAGEPQKQLSKEFVREWLISHGFMGKEGQLIPDMTDEVVLNISE
                     RYQELYQQVTGNSFVPDHSSAEETESRIIDALKKL"
     gene            complement(2683..4956)
                     /locus_tag="PK742_05665"
     CDS             complement(2683..4956)
                     /locus_tag="PK742_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76112.1"
                     /translation="MDSIRTEWVLAAYQSGNKAPDLLLILYADGIHSLKPEQHLPNPD
                     SVSAIIEERRSGKDLIYHITYDDLKLYLARKDDLIMISESSFLVENALNCLEEKNGIS
                     VDEGFQAVHSAPVEGNKFYLYLPSMLSYLQTKGRFPVFPGQELNPPAGWAVLQCKEST
                     GMLLIDGYMEAGTNNQTLHPYLSTASGKSGLSSVLPAETDFVWERMPGQEFRTILQGD
                     QLALDEYQNYWNDWVGSSWATAASMFRDTSIHFEQIFAVSTRKTDALDRLTPMMDAEP
                     LNYREKLVYRMAPSSVLRSMAGISDSTFCTRIDDYLLFSPYLSSIEDCIDAGWSRSVL
                     ADRSAMKLIDSVQLSAWNQLLFLDLDAWSNLFDEPVRDQLVFSDKINPLVVQMNPHNG
                     LFLINAYLPAAVEEEPGGSWAVELDAPVMNGPVALYNQLTETDCFVLQDSLNQLYLID
                     TKGRLLWKKALEQPLVGNISTVDFYNNRKYQLLFATSHAIQLVDMKGNDVEGFPIRIS
                     APVTSGLSAIDYEHDGDYRMFAGCSNGNTYGFYRNGKPLTGWKPLKTGVVTTRVMHCS
                     SNGKDYLYFLTETNELQVRSRSGDLRFPPSSVPPDRLFDPVPDKTREPDGLILMKKGG
                     ELMKINWNGDATVIPFATDAHHALAADINGDGQTDIIYANSAGIFAVEQTGKVLFHTD
                     PGDPLACTLQLLNGANGYRILVNVTTTAQSYLLDEHGNLIETDPFPMSGKAAWTSKPL
                     PILVVSDGQLLQSYRVE"
     gene            5212..5676
                     /locus_tag="PK742_05670"
     CDS             5212..5676
                     /locus_tag="PK742_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HQU76113.1"
                     /translation="MHNTFAKLQGSISAQYTNLWFNVQNMKQVTLIRTATISGAIAVA
                     AGALGAHGLEGRITTELLTAFQTGARYHLVHSVVLLVLALNSGNQYKSGFWLITAGIL
                     LFSGSLYGLALSSLAGLQLSILGPLTPAGGLLLIAGWLVTGWRAAQIKKGGI"
     gene            complement(5707..8307)
                     /locus_tag="PK742_05675"
     CDS             complement(5707..8307)
                     /locus_tag="PK742_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU76114.1"
                     /translation="MKQMKFVLLAAIVLGSAAFTMAQTSGKKIKVNRNVMMADELVKS
                     GSYYNAIDIYLQEFNEDGEPYVAFHLADAYFKARDYKNSETWFQKAFKLDPTNFPEAQ
                     YYYALSMKYNAKYQAAADEFKAFNKTRMRGSQAAYYKRLAKNDLKGCDLALALLEQPE
                     EVEITHLNRNINNPYTDFSPRYNPDNDKLVYASLVSEEVIDMGANKKTELRSRIFEAK
                     QQGEGWAKGEMISGPFNDSPAHTGNGSYSLDGKRFYFTECNPNDSLQMICNIYVSEYA
                     AGEWSKPKPLNDVNAAYGFTTTHPCIGEYKGKEILYFASNREGTVGGMDIWYSEVRNK
                     GAEYTAPQNCGRKINTMGDEITPFYSKKEEVLYFSSNGLINVGGLDIYRVTGGLKSWK
                     NPMNVGFPINSSVDDFYYSTDSKNERTFRAGFFVSNRSGGYSVKGETCCDDIYQYRYI
                     IPPVFTIMGRVFDVKDSAQVDGAAVELLASAGKIDSAASSSAKMFEFYRGSEFENYTV
                     TAIKDGYYQGKAKTSTVGLLLDDTLYVDVYIEKIPIKKEIEVKNIYYELDRAELRPES
                     YPSLDSLYNVMIDNPAIIAEVGSHTDSRGSDSYNLDLSQRRAQSVVNYLVGKGIDSAR
                     LVATGYGETKLKNKCKNGVKCSEEEHAINRRTEFTIIGEIPNTNITYNQSEIDALKAL
                     EREKKEKEDMDRQKRLEDLQQLDEDGGDSNNDGADGGKTNTNTGGGDTGGTADKASVD
                     TKLTKKGAFYEGSAMVNGAEATTYILNMSPSLDIAMVSDEFFVKLKASGTVSDADFKN
                     ENATALSDGTTAKGDIFILKSLQLGDVMLTNVTTKINTTTPQNLVVGVKAVEKGGCTF
                     DSKKVSFKCK"
     gene            complement(8338..9381)
                     /locus_tag="PK742_05680"
     CDS             complement(8338..9381)
                     /locus_tag="PK742_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU76115.1"
                     /translation="MPKFNKLLLVVFLLAGFRSYAQDIHFSQFNAAPLALNPALTGNY
                     ACDWRAGLNYRNQWNSIPAPYVTYSAFADMPIVQGIRGNDKLAAGLLLYNDVSGDGNL
                     SNLSVLASVAYHLALGSPSHYLSLGLQGGMMQKSLDWNNLLFGNQFDGVDFNSATPNF
                     EPYTGDNILNADLNVGLTYKGQFSQNFSLEVGGASNHLLTPGESFLSADTITDRDGTI
                     GNELGMRYVGHMRAIIGFTKDLSLLPTVLYQTQSGAQEMVLGADLGYFLRNPNFPATI
                     YVGLYTRNGDALIPSIAMDYKNFKLGISYDVNTSGLDVATNGRGGFEISLVYTGCILP
                     VVPENYVLPCPRY"
     gene            complement(9393..>10068)
                     /locus_tag="PK742_05685"
     CDS             complement(9393..>10068)
                     /locus_tag="PK742_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU76116.1"
                     /translation="TLQASGATTYLWTPDEFLDTTDGDMVTTTPEETITYTVTGYFYD
                     CTDTRNLTIEVEEPSADAGMDVTIYNGSDTQLEATGGATYVWSPADGLSDPNISDPIA
                     SPEESTTYVVAVTTDLGCTYFDSVNVVVLFEPQVYIPNAFTPNNDGVHDQLNVLIFNE
                     VTPLDFSIYNRWGEKVFQTSNMSSGWDGSVNGVDQEIGTYMYIFVALDERGNEFTLTG
                     TVTLLR"
BASE COUNT         2650 a         2406 c         2238 g         2774 t
ORIGIN      
        1 agttgcacct gtagcgcccc ctcctgtgca ggcttctgaa aataaaccgg ttgtaaagga
       61 atctgataca actacagaaa acggcaacct gatcaccatc aagtctccta ttgttggaac
      121 gttctatcgc tctccgggac ctgataagga agtgtttgtc aaagtaggcg atgatattga
      181 ggttggcagt gtattgtgta tcattgaggc aatgaagcta ttcaatgaga ttgaaagtga
      241 atttgctgga aagatcgtga agatacttgt agatgatgcc agccctgtag aattcgaaca
      301 acccctcttc ctgatcgagc cccgttaata ctgagagggt cagaaaacga gccacatgtt
      361 caaaaaaata ctgatcgcaa accgcggaga gattgccctc agggtgatca gaacagcccg
      421 cgaaatgggc ataaaaacgg ttgcggttta cagcacggca gataaagaca gtctgccagt
      481 cagatttgca gatgaagctg tgtgtatagg tccggctccg tctagagagt catacctgag
      541 cattcccaac atcatggctg cggccgaaat cacaaatgct gatgccattc atcccggata
      601 cgggttcctt agtgagaatg agatgtttgc agacatctgc cagaaatatg gcatcaagtt
      661 cattggtcct cgtgccgatc atattcgctc catgggcgat aagatcacag ccaaagaatc
      721 tatgcgcaag gctggcgtgc cggttgttcc gggtagcgat gggttactta aggatgtagc
      781 agacgcccgt gaacaggcca aaaagatcgg ttatccggtc atgctaaaag ctactgccgg
      841 tggtggtgga aagggaatgc ggatcgtttg gggtgagaaa gatctggaaa atgcatatgc
      901 cagtgcaagc accgaggcaa aagcttcttt caacaacgat ggcctctata tggagaaatt
      961 cattgaagag ccaaggcaca ttgaaatcca ggttgccggc gatcaatacg gcaaggcctg
     1021 ccaccttagt gaacgtgact gttctattca acgcagacat cagaagcttg tagaagaaag
     1081 cccctctcct tttgtggatg aaaagttacg ggaaaagatg ggcgaaattg ctgtcagagc
     1141 ggccgaaagc attggctatg agggtgtcgg aaccattgaa ttcctgactg acaagcataa
     1201 gaatttctac ttcatggaga tgaacacccg tattcaggtg gaacatcctg ttacagagga
     1261 agtagttgat tatgacctga tcaaggaaca gatcaaatta gctgccggcg aaccgatcag
     1321 tgggaagaat tattatccca tattgcatgc catggaatgc cgcatcaacg ccgaagaccc
     1381 gtcacaggga ttcagaccca gtccgggtaa gatcgtcaac ttccattcac caaaaggtca
     1441 tggtgttcgt gtggacacag ctgtatatgc cggctatgtg atccccccat attatgactc
     1501 tatgatagcc aaactcattt gtcgtgcacg tacccgcgat gaggttattc tgaagatgca
     1561 gcgtgcactg gatgaattca ttgtagaagg agtaaagacc actgtaccat tccacaagaa
     1621 actgatgcga gatgagcgat tccgtgccgg tgtatttgac acgtctttca tgaacagctt
     1681 tgacctgact gatctgcctt cctgataact acagcttttt cagtgcatca atgatgcgtg
     1741 attctgtttc ttcagccgaa gaatggtcag gaacgaaact atttcctgta acctgctggt
     1801 ataattcctg gtatcgttca ctgatgttca gtaccacttc atcagtcata tcagggatca
     1861 gctgaccctc tttccccatg aatccgtggc tgatcagcca ttctcttaca aattctttag
     1921 agagttgttt ttgtggctct ccggcagcct gtctatcctc ataacctgct gcatagaagt
     1981 aacgggaaga atcaggtgtg tggatctcat ccatcagcat aatgttacca ttgtgtacac
     2041 caaattcata tttggtatca accaggatca ggccttgagc agctgcatat cttgttccga
     2101 atgcaaataa ctgacgggta attgtttcca gctgcagata ttctgcttcc ggcacaatac
     2161 cacgtgctat gatctcttct ttgctgatat cttcatcgtg gccttcagat gctttggtag
     2221 tgggtgtaat gattggttcg ggaaaggggt catgctcacg gagtccatca ggtacctgca
     2281 ccccgcaaat agttctttta ccactgctat attctctcca tgcatgccct gtaaggtatc
     2341 ctctgatgac catctctact ttatagggct gacatcgcaa accgatggct actctggggt
     2401 ctggtgtttg cagtaaccaa accggaacaa tatgctgcac ctgtttcaga aatcctgcag
     2461 cgatctgatt caggatctgt cctttatagg ggattggtct gggcagaact acatcaaagg
     2521 ccgagatcct gtcgctggca accatgacca gcagatcttc accgatggaa tagacatctc
     2581 ttacttttcc tctgtaaaac cctgtttgcc cgggtagttg ataatgtgtt tctgcaaccg
     2641 gcatgtgcac ttgatttaca tccaaaagta cacagaagcc ccctattcga ccctatagct
     2701 ctgcaggagc tgtccatctg acaccaccaa aatgggcagt ggcttgctgg tccacgcagc
     2761 tttgccactc atgggaaaag gatcagtttc aatcaggttt ccatgctcat ccagcagata
     2821 actctgtgca gttgtagtga cattgacaag gatccggtat ccatttgcac cattcaacag
     2881 ttgaagtgtg caggccagtg gatcgccggg gtcagtatga aacagtacct ttcctgtttg
     2941 ttcaacggca aatatccctg cactatttgc atatataata tctgtctggc catctccatt
     3001 aatatcggcc gccaaagcat ggtgtgcatc tgtagcaaac gggataactg ttgcatcgcc
     3061 gttccaatta attttcatca gctcaccgcc tttcttcatc aatatcaggc catccggttc
     3121 tctggttttg tcaggaactg ggtcaaaaag cctgtcgggt ggaactgatg aaggaggaaa
     3181 gcgtaaatca ccacttctgc ttcttacctg caattcattt gtttctgtaa gaaaatacaa
     3241 ataatctttg ccgttactgc tgcaatgcat gacgcgggtg gtcactacac cggtctttag
     3301 aggtttccat ccggtaagcg gcttgccatt tctatagaac ccatatgtat ttccattact
     3361 acaacctgca aacatcctgt agtctccatc atgttcataa tctatagctg acaggccgga
     3421 tgtaacgggt gcgctgatgc gaatgggaaa accttccaca tcattacctt tcatatctac
     3481 cagctgaata gcatgactgg ttgcgaacag caactggtac tttcggttgt tgtagaaatc
     3541 aactgtagat atatttccaa caagcggttg ctccaacgcc ttcttccaca acaaccggcc
     3601 tttcgtatcg atcagatata gctggttcag gctatcctgc agtacaaaac aatctgtttc
     3661 tgtaagctga ttatagagcg ccaccgggcc attcattacc ggagcatcca gttcaacagc
     3721 ccatgaacca ccaggctctt cttccacagc ggcgggcagg taggcattga tcaggaataa
     3781 cccgttgtga ggattcatct gcaccaccaa tgggttgatc ttgtcagaaa agaccaactg
     3841 atctctgaca ggttcatcaa acaagttgct ccatgcgtca agatcaagaa acaatagttg
     3901 gttccaagca gaaagctgaa cgctgtcaat cagtttcatt gcagaccggt cagcaaggac
     3961 agatctgctc cagccggcat caatacagtc ttcaatggaa gacaggtatg gagagaacag
     4021 caaataatca tctatgcgtg tgcaaaaagt tgaatcagat attcctgcca tcgatctaag
     4081 aacagaagaa ggtgccattc tgtacaccaa cttttcccgg taattcagag gctctgcatc
     4141 catcatgggc gtcagtctgt ccagggcatc tgtttttcgg gttgacactg cgaatatttg
     4201 ttcaaaatgg attgatgtat cccggaacat agaggccgct gttgcccacg aagagcctac
     4261 ccagtcattc cagtaatttt gatattcatc cagtgccagc tggtctccct gcaatatggt
     4321 tctgaattct tgcccgggca tgcgttccca aacaaaatca gtttcggcag gtaatacgga
     4381 tgacaagccg gacttacccg aagcagtaga aagatatggg tgcagtgtct ggttattggt
     4441 gcctgcctcc atatacccat caatgagcaa catgcctgta gattctttgc attgcaacac
     4501 cgcccatcct gcaggtggat tcaactcctg tcctggaaaa accggaaatc ttcctttagt
     4561 ttgaagataa gatagcatgg acggcaggta caagtaaaat ttattgcctt ctaccggagc
     4621 agaatgaaca gcctgaaaac cttcatccac agagatacca ttcttctctt ccaggcagtt
     4681 caaagcattt tcaaccagaa aggaagattc tgatatcatg atcagatcat cttttctggc
     4741 caaatacagt ttcagatcat cataggttat atggtaaatc agatctttgc ctgacctgcg
     4801 ctcttcaata atagcagaaa cgctatcagg attgggcaaa tgctgttcgg gtttaaggct
     4861 atggatacca tcggcatata aaatcagcaa gaggtctggt gctttattac ctgactgata
     4921 ggcagccaga acccactcgg tcctgatgct gtccatgtga aatgcatcag ttaggaacct
     4981 caatgttttt gtactctgta tcagatcatc cagcaatgga aaagaatact gattgctcag
     5041 agaatcgcta agccacatta gctgaccttg gccaccagtt actaaaacag gtgaagcaaa
     5101 aatgccttgc cagggatcgc tatacttctt ccagcgtatc caattctgct gccaggcaat
     5161 aaccgacact gccgccaaaa cgatcagcgc tgctccaaga atctttttca tttgcacaat
     5221 acctttgcaa aactacaagg gtcaatatca gcccaataca caaacctttg gttcaatgta
     5281 cagaatatga agcaagtaac actgatcaga actgcgacca tttccggagc tatagcggta
     5341 gctgccgggg cacttggggc acatggactt gaaggacgaa taacgacaga attactgacg
     5401 gcttttcaaa caggagcccg ttaccattta gtacacagtg tcgtgctttt ggtgctggct
     5461 ctaaattcag gtaaccaata caagtcaggt ttttggctaa tcacagcagg cattctgctc
     5521 ttctccggat ccttgtatgg tttagcgcta agctccctgg caggtttaca attgtccata
     5581 ctaggtccgc tcactccggc aggcgggttg ctgctgattg ccggttggct ggttacaggc
     5641 tggcgggctg cacaaataaa aaagggcggt atttgatacc gccctgtatt gctaacctga
     5701 tttgggttat ttacacttga aacttacttt cttggagtca aaggtacatc cgcctttttc
     5761 aactgccttt acacctacta ccaggttctg aggagtggtg gtgttgatct tggtagtaac
     5821 atttgtcagc atcacatcgc ccaactgtaa tgacttcagg atgaaaatat cgcctttagc
     5881 agtagtacca tcagacagtg ctgttgcatt ttcattttta aaatcagcat cagagactgt
     5941 tccggaggct ttcagcttca caaagaattc atcgctgacc attgcgatat ccagacttgg
     6001 agacatgttc aggatatagg ttgtggcttc agcaccattt accatggcag aaccttcata
     6061 gaatgcacct ttcttggtaa gcttggtatc tacgctggct ttatcggcag ttccaccggt
     6121 atcgccacca ccagtattgg tgttggtctt gcctccatca gctccatcat tgttgctgtc
     6181 tcctccatcc tcatccaact gctgcagatc ttccaagcgc ttctgacgat ccatatcttc
     6241 tttctccttc ttctcacgtt ccagagcttt cagtgcgtcg atctcagact ggttgtatgt
     6301 aatgtttgta ttcgggatct cgccaatgat ggtaaactcc gtgcgacggt taatagcatg
     6361 ttcttcttca gaacatttta ctccgttctt acacttattc ttaagttttg tttcaccata
     6421 gcctgtagca accagtcggg ctgaatcaat tccctttcca accaggtagt tcactacaga
     6481 ctgggcacgg cgctggctga ggtccaggtt ataggaatca gaaccgcgac tatctgtatg
     6541 agatccaacc tctgcgataa tggcagggtt gtcaatcatt acattataca atgaatcaag
     6601 tgaaggatag gactccggac gaagttcagc tctatccagc tcataataga tgttcttgac
     6661 ctcgatctct tttttgatag ggatcttttc aatatacacg tccacataca aagtgtcatc
     6721 cagcaggagt ccaactgtac tggtttttgc cttgccctgg tagtagccat ccttgatggc
     6781 tgtaactgta taattctcaa actctgaacc ccggtagaat tcaaacatct tcgcagaaga
     6841 gcttgccgca gaatcgatct taccggcaga tgccagcaac tctaccgctg cgccatcaac
     6901 ctgtgcacta tccttcacgt caaaaacacg acccatgata gtaaatacag gcggaatgat
     6961 atatctgtac tgatagatat catcacaaca ggtctcaccc tttacagagt atcctccaga
     7021 acggttagaa acaaagaatc ctgcacggaa agttctttca ttcttgctgt cagtggaata
     7081 atagaaatca tctactgagc tattaatagg aaatccaaca ttcattgggt ttttccagct
     7141 tttcagtcca cctgttacac gatagatatc caatccacct acattgatca ggccatttga
     7201 gctgaaatac agcacttctt cctttttgct gtaaaagggt gtgatctcat cacccatggt
     7261 gttgatctta cggccacagt tttgcggagc tgtatattca gcgcccttat ttcttacctc
     7321 tgagtaccag atatccattc caccaacagt tccttccctg ttagacgcaa aatagaggat
     7381 ctctttccct ttgtactctc cgatacatgg atgagtagtt gtaaacccgt aggcagcatt
     7441 gacatcgttc aatggctttg gcttactcca ttctcctgct gcatattcac ttacataaat
     7501 gttacagatc atttgcagac tatcgttcgg gttacactct gtaaagtaga atcgtttacc
     7561 atccagagaa tagcttccat ttccggtatg tgccggagaa tcattaaagg gacctgatat
     7621 catctcaccc ttggcccagc cttcaccttg ctgcttggct tcaaagatgc ggctgcgcaa
     7681 ttcagtcttt ttgtttgctc ccatgtctat cacttcttca gacaccagtg aagcatacac
     7741 cagcttatcg ttgtcgggat tatatctggg agaaaagtct gtataggggt tattgatatt
     7801 gcggttcagg tgagtgatct ccacttcctc aggttgctcc agcagagcga gtgccagatc
     7861 acaacctttc aggtcattct ttgccaaccg cttataataa gctgcctgag aaccccgcat
     7921 tctggtttta ttgaatgcct tgaactcatc agctgctgcc tgatatttgg cattatactt
     7981 catgctgaga gcatagtaat actgggcttc cggaaagttt gtcggatcga gtttgaaagc
     8041 cttctgaaac catgtctcag aattcttata atcccgggcc ttgaagtagg catctgccag
     8101 gtggaaagcc acataaggtt cgccgtcttc attaaactcc tgtaaataga tatcaatagc
     8161 gttatagtat gagcctgatt ttaccagttc atctgccatc attacgttcc tgttcacttt
     8221 gatcttcttc ccactagtct gggccatagt gaacgctgca ctgcccaaca cgatggcagc
     8281 cagcagaacg aatttcattt gtttcatatg ctaagtttac gctttacttt aggtagatca
     8341 atatcttgga caaggcaata catagttttc aggtacaacc ggcaagatac atccggtgta
     8401 caccaggctg atctcaaaac caccgcgtcc gttagtggct acgtccagtc ctgaggtatt
     8461 cacatcatag ctgataccca gtttaaagtt cttatagtcc atagcaatac ttggtatcag
     8521 tgcgtcacca tttctggtgt acagccctac atagatcgta gcggggaagt tcggattacg
     8581 gaggaagtac cccagatctg cgcccagcac catttcctga gctccgcttt gtgtctgata
     8641 caacactgtt ggtagcaggc tgaggtcttt ggtaaagccg atgatagctc tcatatgacc
     8701 tacgtatctc atacccagtt cattgccaat tgtaccatcg cggtctgtga tggtatcggc
     8761 agacaggaat gattctccgg gagttaacag gtgattgctg gcaccgccta cttccaggct
     8821 gaagttttga gagaactgcc ctttgtaagt aaggcccaca ttcagatctg cattgaggat
     8881 attatcgccg gtataaggtt caaagtttgg cgtagcgctg ttgaaatcaa ctccatcaaa
     8941 ctgattacca aaaagcagat tgttccagtc cagtgatttt tgcatcattc ctccctgtaa
     9001 accaagagag aggtaatggc tcgggcttcc gagtgcaagg tggtaagcta ctgaggccag
     9061 cacagagagg tttgataagt taccatctcc tgacacatca ttatacagca acaagcctgc
     9121 tgcgagttta tcgttcccac ggataccctg tacaatgggc atatcagcga atgccgaata
     9181 ggtcacataa ggagccggaa tactgttcca ctggttcctg tagttcagcc ccgcacgcca
     9241 gtcgcaggca taattaccag taagtgccgg gttcaatgca agcggtgcgg cattgaattg
     9301 cgagaagtga atatcctgag catatgaccg gaatccggcc agcaggaaaa cgacaagtag
     9361 cagtttgttg aatttaggca tatatgctgt ttttatctaa gtaatgtgac tgttccggtt
     9421 agtgtgaatt catttccacg ttcgtccaat gctacaaata tatacatgta ggtgccaatt
     9481 tcctgatcca ccccgttcac actgccatcc caaccggacg acatgttgct ggtctggaac
     9541 accttttctc cccaacggtt ataaatagag aagtcaagtg gcgttacttc attgaaaatg
     9601 agcacattaa gttgatcgtg aaccccgtca ttgttgggtg tgaaggcatt gggtatatac
     9661 acctggggtt caaagagaac caccacgtta actgaatcaa aatacgtaca acccaggtct
     9721 gtggtaacgg ccaccacata ggtagtagat tcttccggag aggcgatcgg atcagaaatg
     9781 ttggggtcac ttaagccatc tgcaggcgac cagacatagg tcgcaccacc tgttgcttcc
     9841 aactgggtat cgctgccatt ataaatggtg acatccatgc cagcatctgc cgaaggttct
     9901 tccacctcaa tggtcaggtt tctggtatcg gtacaatcat agaagtaacc ggttaccgtg
     9961 taggttattg tttcttcagg tgtggtggtc accatatctc catctgtagt atccagaaat
    10021 tcatcgggag tccagagata ggtggtcgct cccgatgcct gtagtgtc
//
LOCUS       DAOMAX010000076        10059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_911_length_10059_cov_4.011995, whole genome shotgun sequence.
ACCESSION   DAOMAX010000076 DAOMAX010000000
VERSION     DAOMAX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_10059_cov_4.011995"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="gatB"
                     /locus_tag="PK742_05690"
     CDS             complement(<1..594)
                     /gene="gatB"
                     /locus_tag="PK742_05690"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613106.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HQU76117.1"
                     /translation="MNKESYEAVIGLEVHAQLLTKTKAYSSDIAEYGGDPNTHVSVVT
                     LGHPGTLPVFNKRTAELAITMGLATHCKIREHNLFARKNYFYADLPKGYQITQDQTPI
                     CTEGYVEIGESGKEKRIGITRIHMEEDAGKSIHELDPYFTLIDLNRAGTPLIEIVSEP
                     DLRSGEEAYEYLTEIRKLVRYLAICDGNMEEGSLRCDA"
     gene            675..2075
                     /locus_tag="PK742_05695"
     CDS             675..2075
                     /locus_tag="PK742_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76118.1"
                     /translation="MSTRLLLLFFSVFFLLAGRQSPVLGQAGNIATEAQNGSYQLFSL
                     TDLGIFRQARFQSVVNLSAGSSTWAYVEADGNYTPNWRPYFSGLTLAGYDQTIAPIGG
                     TASATYNSGYGSFWGDDGLMPAVTSGNYYTFNITEYSKPGDTVNEYMGVLETSYDPVS
                     FTSLTQSPVSGIDRSTYVTITVSLSDMPSAGEYLYLRYAPTYNFTGSNFLDMYVSGLT
                     ASATIPCQDQDSIYYYVYSSNRTKPQIATEVTAHGQVAHDMLTLNLINNGGPNYFYTI
                     LPVNSCDILPVTWLDFTAEAYEQTVGLNWWTSSESNSDHFKVQRSTDGSDWEDIGQVL
                     AAGQSFQTIPYSYTDDAPLSGRAYYRLLQVDQDGTSDASEIRQVFFQIQDQIHIFPNP
                     SSGTFHISMQGMQGEQAYCLITDMAGKTVYAERFFPDGMMTNKTLNTGLPDGVYQVII
                     TAGQLDICRQQLVIQR"
     gene            complement(2062..4776)
                     /gene="alaS"
                     /locus_tag="PK742_05700"
     CDS             complement(2062..4776)
                     /gene="alaS"
                     /locus_tag="PK742_05700"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQU76119.1"
                     /translation="MYALFPVGNGVTKVSNFNVKRQALLDSDMQTAANIRQEFLKFFE
                     EKGHKIVPSAPIVVKDDPTLMFTNAGMNQFKDLFLGNREASETRVADTQKCLRVSGKH
                     NDLEEVGVDTYHHTMFEMLGNWSFGDYFKADSIAWAWEFLTDRMGIDKDRLYVTVFEG
                     DDSEGLAFDQEAYDEWKKHISEDRILKGNKKDNFWEMGDTGPCGPCSEIHVDVRTEQD
                     RKAQDGRELVNASHPEVIEVWNLVFMQFNRKADRSLVNLPARHVDTGMGFERLVRTLQ
                     GKQSNYDTDIFQPVIRHTETLCNKKYGADEKTDIAFRVIADHIRAVAFCIADGQLPSN
                     NKAGYVVRRILRRAVRYGYSFLDLRDPFFHTLVPVLAEQMADAFPELKSQLALVERVI
                     QEEETTFLRTLENGLKKLHDVVNDLQAAGEKTIPGQVVFELYDTFGFPDDLTALLARE
                     EGLLVDEAGFETALTEQKERSRKAGTSETSDWTIVHDGKDVQFTGYDSTSGDAQILRM
                     REVKQQQKKLYQIVLDKTPFYAESGGQTGDTGILNIGGETLQVLDTRKENDLIIHFTN
                     KLPAAPGGVVHAEIDAGRRSETARNHSATHLLHAALRRILGDHVQQKGSYVGPDRLRF
                     DFSHFAKMTEEEIAEVESMVNEKILENVPLDERRSVPLDEARMLGAMMLFGEKYGDHV
                     RVITFDPGYSRELCGGIHVQATGQIGLFKIVSESAVAAGIRRIEAITGHASMQYANNH
                     IETVNRIGELLHAKDPAKGVEQLLEEQQQLKKEIEQFQLQALQSEKNRLQKQAFTKNE
                     IRCIIGKVSVANAELLKKLAYDLRNEEPNLFAVLGAEIADKAQLAVMISDEVVNSRGL
                     HAGQLVKELGKFIQGGGGGQPHFATAGGKDPSGIEAALEAASAKLSAE"
     gene            4800..5771
                     /locus_tag="PK742_05705"
     CDS             4800..5771
                     /locus_tag="PK742_05705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQU76120.1"
                     /translation="MKKTKYYYNPNSLRFEKLEISRLRVALTIFGWVCTALVFAGIIV
                     WIAFTWFDSPKERYLKKQIAEMEFEYELMTDKLDTMAIILNELQYRDDNIYRTIFEAD
                     PVSRNERLVGIGGSERFGRMSNLDNAELVKSTALKLELIKRRMVVQSKSYDELSDMVR
                     DKEKLLAHTPAIQPVSNKDLERISSGFGYRIHPVYKFAKMHEGLDFTAPRGTDVFATA
                     DGVVETANISSRGYGNEIVINHGYGYKSRYAHLNGFAVKQGQKVKRGELIGYVGNTGL
                     STAPHLHYEVEKDGIKVNPINFFYNDLTPEQYLRVIDLANQYNQSYD"
     gene            5808..6143
                     /locus_tag="PK742_05710"
     CDS             5808..6143
                     /locus_tag="PK742_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQU76121.1"
                     /translation="MPYKEKEIEKRYWTIGEVADELQVSASLLRFWESEIGIIQPKKN
                     RKGDRFYSRKDIDTLRLIYHLVKEKGYTLKGARQQLLHSKDGEEAKFEAIESLKKIRE
                     FLVQIRDQL"
     gene            complement(6144..7844)
                     /locus_tag="PK742_05715"
     CDS             complement(6144..7844)
                     /locus_tag="PK742_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76122.1"
                     /translation="MPAGRFILFLFLVTVLAACDLQAQNTNIPLNGYGDRLLRRYAIL
                     DTSLDLHTSMRPYGRKDISSWMQKNYGKPTNTLESYDSRDVFNTFWLRDDASEFLEGF
                     QTGRNKPLLGIFFKESANFFQTEGDHYFVNLNPVIYNSFGGTSDSGKLVFENTRGLEV
                     RAGIDDRVGFYVFATDNQATYPGYVTDAANLNVQKVVPSLGWSKPFKAQGFDFPVSRG
                     HMDVQLFEHLSLQVGQDKMFVGDGVRSLVWSDNAREPFYVRLQTQFWRIRYQANFLSL
                     INMNQPKLPDNRWRKKYASLHQLNVEVLPNLDLGIFETVVFQRNDSLGYPTGFDLNYA
                     NPLIFYRSVEWGLGSPDNVLLGLLWKWTFLQQYAFYGQFVLDELRFFDLTKATGWWGN
                     KYALQTGLQYINAFGLDFLDLQYEMNLVRPYMYSYSNDNGSSYTHYAQAIAHPLGANF
                     AEHLFTVNYQPLAQWNLSNVFFTAVSGADSAGMNYGADIFLNNDSHVQDYGNDLLQGF
                     RTTTLYNETTLSWQFWHNTFIDARVVYRSSTGTQPIKDQEWFFSLGVRMNAVIPTFRF
                     "
     gene            7887..9284
                     /locus_tag="PK742_05720"
     CDS             7887..9284
                     /locus_tag="PK742_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76123.1"
                     /translation="MRKQLLWIVFLPLTTLAQHLGFNGAGFFENSNEEVIRYMQSWTG
                     DFTIRVPGGAISKFHDPYNVRQGWGMTDESVQSWFARDGFDEDGNGLDKWLRKTGEQP
                     DHSYLDDLIALQKKFPKMRVLYVLNVLNSTPEANMQAIRYLVQNGVRVTGVEAGNEIY
                     GKYSSFDEYARDFQPIFDLLDREFPDISKGLVAGANVQRKQLQQWNDALAAYRGDYDA
                     VILHYYYTARELGSAYDQLEKVKYTPGKYYPALDQAFQAAKLAMTKEDMIGRGIQYAK
                     EQFPGKKIWITEWNTKPSDKLNNTLLNAGWQYEELLRHRSDVEYLLVHNGAGPDKYAM
                     MSRRQSKYDSQPEYMIRRAAFYSFQLALEAAYSKPLERGKNTYINGGQRSQYWFSNLE
                     KDTWEPAIVWNKDQFKDVQIHFVHAPYLYSAAGVAGYMSKGSTPSYEIDGISTMPFTG
                     SIPAYSYGYIEWIPR"
     gene            9360..>10059
                     /locus_tag="PK742_05725"
     CDS             9360..>10059
                     /locus_tag="PK742_05725"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HQU76124.1"
                     /translation="MAMQFKDIQHLYLRGGFGATPSQIAYRTGKERTDLVEELFAASR
                     SYRGLNHLEDPVKGKKVGNLRILFMLLRSQDQMRELNLEWIRLMAGTNAALREKMTFF
                     WHDHFATSTPLAWLMQVQNNTLRKHALGNFRTMLHAISKDPAMIVYLNNQQNKKDHPN
                     ENFAREVMELFSLGIGHYTEQDVKEAARAFTGWTVNKKGEYEFDEKEHDEGEKTVLGR
                     TGNWNGNDIIDMLLE"
BASE COUNT         2572 a         2448 c         2439 g         2600 t
ORIGIN      
        1 ggcatcacag cgcaggctgc cttcttccat attgccatcg cagatggcca ggtagcgcac
       61 cagtttgcgt atctccgtca ggtactcata ggcttcttca cctgaacgca ggtcgggttc
      121 actgacgatc tctatcaaag gggtacctgc ccggttcagg tcgatcaggg tgaaataagg
      181 gtccagttcg tggatgcttt ttccggcatc ctcttccata tgtatgcggg tgatgccgat
      241 gcgcttttct ttcccggatt ctcctatttc cacataccct tcggtacaga tgggcgtctg
      301 gtcctgggtg atctggtatc ctttgggaag atccgcatag aaataattct tgcgggcgaa
      361 cagattatgc tcacggatct tacaatgcgt ggccagaccc atcgtgatgg ccagttcagc
      421 ggttcgcttg ttgaataccg gcaaagtgcc gggatgcccc agggtcacta cactgacatg
      481 ggtattcgga tcgcctccgt attccgcaat atcagacgaa taggccttgg tctttgtgag
      541 cagctgggcg tgcacctcca gaccgatcac cgcttcataa gattccttgt tcatagagcg
      601 gggcaaagat acgcagaagc agcgccgatg aacaatttgg cggtctgttg ttacttttat
      661 cagttttcaa gatcatgagt acccgtctgc ttctgttgtt tttttcggtt tttttcctgc
      721 tggccggccg gcaaagtccg gtattggggc aggccggcaa tattgctaca gaagcccaaa
      781 atggcagcta ccaattattt tcgctcacag acctcggcat cttcaggcag gcccgattcc
      841 agtctgtagt caatctgtct gcaggatctt ctacctgggc ctatgtggaa gctgatggca
      901 attatacacc taactggagg ccctattttt ccgggctgac cctggcaggc tatgaccaga
      961 ccatcgcacc catcgggggt acggcaagtg ctacctacaa ttcaggctat ggtagtttct
     1021 ggggcgacga tggcttgatg ccggctgtca catcaggcaa ctactataca ttcaatatta
     1081 ctgagtacag caagcctggc gatacggtca atgaatacat gggggtgctg gaaacttcct
     1141 atgatccggt ttcctttacc tcacttaccc agtctccggt ttcaggcatt gaccgatcta
     1201 cctatgtgac catcacagtg tccctcagcg atatgccatc ggccggagaa tacctgtatc
     1261 tgcggtatgc acccacctac aatttcaccg gcagtaattt ccttgatatg tatgtaagcg
     1321 gattgactgc atctgccacc attccctgtc aggatcagga ttccatctat tattatgtgt
     1381 attccagtaa ccgtaccaaa ccccaaatag ccacagaggt aactgcccac gggcaggtag
     1441 cgcatgacat gctgaccctc aacctgatca ataacggagg accgaattat ttctatacta
     1501 ttcttcctgt taactcctgt gatattttgc cggtaacctg gctggatttt acagctgaag
     1561 cctatgagca aactgttggc ctgaactggt ggacctcatc agaatccaac agcgatcatt
     1621 tcaaagtgca aagaagtacc gacggttctg actgggagga tatcgggcag gtgctggccg
     1681 caggacaaag ttttcaaaca ataccgtatt cctatacaga tgatgctccc ttgtcaggca
     1741 gggcctatta ccggttgctg caggttgacc aggatggaac atcagatgcc agtgagatca
     1801 gacaggtctt tttccaaata caagatcaga tacacatatt ccccaatcct tcgtcaggaa
     1861 cattccatat cagcatgcaa ggaatgcagg gtgaacaggc ttattgtctg atcacagata
     1921 tggccggaaa aactgtgtat gcagaacgct tcttccccga cggaatgatg accaataaga
     1981 ccctgaacac cggtttgcct gatggcgtat atcaggtgat catcaccgca ggacagctgg
     2041 atatctgcag gcaacaactg gtcattcagc gctgagcttt gcagatgcag cttccagcgc
     2101 tgcttctatt ccggaaggat ctttaccacc ggcagttgca aaatgtggtt gtccgccgcc
     2161 accgccctga atgaacttac ccagttcctt caccagctgc ccggcatgta gtcctcttga
     2221 attcaccacc tcatcgctga tcatgaccgc cagctgtgct ttatcggcga tctcagcgcc
     2281 cagcactgcg aacagattgg gttcttcgtt gcgcagatcg tatgcgagtt tttttaacag
     2341 ttctgcgttt gccacagata cctttcctat gatacagcga atttcatttt tcgtaaaggc
     2401 ctgtttttgc aaacggttct tttctgactg aagagcctgc agctgaaact gctcgatctc
     2461 ttttttcagt tgctgttgtt cttccagcaa ttgttccaca cctttggccg gatccttggc
     2521 atgtagcaat tcaccgatcc tgttgacagt ttcgatgtgg ttattggcat actgcatgga
     2581 cgcatgcccg gtaatagctt ctatccgtct gatgccggcg gcaacagcag actcagaaac
     2641 gatcttgaac agcccgatct ggccggtggc ctgcacgtga ataccgccac acaattcacg
     2701 gctgtaccca gggtcaaaag tgatgacccg cacatgatcg ccgtactttt cgccgaataa
     2761 catcatggca ccgagcatac gggcttcatc aagtggtacg gaacggcgtt catccagagg
     2821 tacattctcc aggatctttt cattgaccat gctttctacc tctgcgatct cttcttcggt
     2881 cattttggca aaatgtgaga agtcaaaacg caggcggtcc ggccctacat agcttccttt
     2941 ctgttgtaca tgatcaccca gaattcttct cagcgctgcg tgtagcaggt gggtggcact
     3001 gtgattgcgg gcagtttcag acctcctgcc tgcatctatc tcggcatgaa caacaccacc
     3061 cggagctgca ggaagcttat tggtgaagtg aatgatcagg tcattttctt ttctggtatc
     3121 cagcacctgt aaagtttcgc cgccaatatt cagtattccg gtatcacctg tttgtccgcc
     3181 gctttctgca taaaaaggtg tcttgtcaag aacgatctga tagagtttct tttgttgttg
     3241 tttaacctcc ctcattcgga gtatctgtgc atctccggaa gtgctgtcat atccggtaaa
     3301 ctgcacgtct tttccatcat gaacaatggt ccagtcgctg gtttcagaag tacctgcctt
     3361 tcggctgcgt tccttttgtt cggtcagtgc agtttcaaag ccagcttcat ccaccagcaa
     3421 tccttcttct ctggcaagta aggctgtcag gtcatccgga aagccgaagg tgtcatacag
     3481 ttcgaatacc acctgaccgg gaatggtctt ttcgcctgct gcctgcagat cgttcacgac
     3541 atcatgaagt tttttcagac cattctccag ggttcggagg aaggtggtct cttcttcctg
     3601 aatgacacgt tctaccaaag ccagctggct ttttagttca ggaaaagcat cagccatttg
     3661 ttcggcaagt acaggtacca gtgtatgaaa gaaagggtcg cggagatcca gaaacgaata
     3721 accatagcga acagcccggc gcaggatcct gcgcaccaca tagccggcct tattgttgga
     3781 gggaagttgt ccatcggcta tacagaaagc aactgcacgg atatgatctg ctatgacacg
     3841 gaaagcaata tccgtttttt catcggcacc atattttttg ttgcaaagtg tttccgtatg
     3901 tctgatgaca ggctggaaaa tatcggtatc gtaattggac tgtttgccct gcagggtacg
     3961 caccagacgt tcaaatccca taccggtgtc cacatgtctg gcaggcaggt tgacaagaga
     4021 tctgtcggcc ttccggttga actgcatgaa gaccagattc caaacctcta tcacttccgg
     4081 gtggctggca tttaccagtt ctctgccgtc ctgcgctttt ctgtcctgtt ctgttctcac
     4141 atctacatgg atctctgaac aagggccgca gggtccggta tcgcccatct cccagaagtt
     4201 atctttcttg tttcctttta atatgcggtc ttctgaaatg tgttttttcc attcatcata
     4261 ggcttcctgg tcgaaagcca ggccttcact gtcatcacct tcgaatacgg tcacatacaa
     4321 acggtctttg tctattccca ttctgtcggt caggaattcc caggcccagg ctatactgtc
     4381 agccttgaag taatcgccaa agctccagtt gcccagcatc tcgaacatgg tgtggtgata
     4441 agtatccaca cctacttctt ccaggtcatt gtgtttgcct gacacccgta agcatttctg
     4501 tgtatcagcc acccgtgttt cagaagcttc ccggttaccc agaaagaggt ctttgaactg
     4561 attcatcccg gcattggtga acatgagggt agggtcatcc ttcaccacga tgggtgcgga
     4621 agggacgatc ttatgtccct tctcttcaaa gaatttcaga aactcttgtc tgatgttggc
     4681 cgctgtctgc atgtctgaat ccaataatgc ttgtcgtttg acgttgaaat tgcttacttt
     4741 cgtaaccccg tttccaaccg ggaacagggc gtacaaaggt aaggatttca ggccttagga
     4801 tgaagaagac caagtactac tacaacccga acagccttcg ctttgagaag ctggagatca
     4861 gtcgcctgcg ggtggctctg accattttcg ggtgggtatg tacagccctg gtttttgccg
     4921 ggatcatcgt ctggatcgct ttcacctggt tcgattcgcc caaggagcgc tacctcaaga
     4981 agcagatcgc cgaaatggaa ttcgagtatg agctgatgac agacaagctt gacaccatgg
     5041 ccatcatcct gaatgagctt cagtaccgcg atgataacat ctacaggacc atcttcgaag
     5101 ccgatccggt ttcacgtaac gaaaggttgg tggggattgg cggttccgaa agatttggcc
     5161 gaatgagcaa tctggataat gccgaactgg taaaaagcac cgcactgaaa ctggaactta
     5221 tcaaacgacg gatggtcgta cagagcaaga gctatgatga gctgagtgac atggttcgcg
     5281 ataaggagaa actgctggct catacacctg ccattcagcc ggtttccaac aaagacctgg
     5341 agcgcatctc ttccgggttc ggataccgca tccatcctgt ttacaaattt gccaagatgc
     5401 atgaaggcct tgacttcaca gcaccccgtg gcacagatgt atttgctact gccgatggtg
     5461 tggtggaaac tgccaatatc tcttcccgtg gatatggcaa tgagatcgtg atcaaccacg
     5521 gctatggtta taaatccaga tatgcccacc tgaacggctt tgctgtaaag caggggcaga
     5581 aggtcaaaag gggtgaacta attggctatg taggcaatac aggtctcagt accgcaccac
     5641 atcttcatta tgaagtggaa aaggatggca tcaaggtcaa cccgatcaac ttcttctata
     5701 acgacctgac ccctgaacag tacctccggg tcatagatct ggccaatcag tacaatcagt
     5761 cttacgattg acatgcctgc ctgtagtatt tgattacctt tagcatcatg ccttacaaag
     5821 agaaagagat cgagaagcgt tactggacca tcggtgaggt ggcagatgag cttcaggttt
     5881 cagcttccct gcttcgattc tgggaaagtg agatcggcat tattcagccg aagaagaaca
     5941 gaaaaggcga caggttctat tccagaaaag acatcgatac cctcaggctt atttaccatc
     6001 tggttaaaga aaaaggatat accctgaaag gtgcccgcca gcaactactg cacagtaaag
     6061 atggtgaaga ggcgaaattt gaagccattg aatcactgaa gaagatcagg gaatttctgg
     6121 ttcagatccg cgatcagctc tgatcagaac cggaaggtag ggatgaccgc attcattcgc
     6181 acccctaaac tgaagaacca ttcctggtct ttgatgggct gagtgccggt gctgcttctg
     6241 taaaccaccc ttgcatcaat aaaagtgtta tgccagaatt gccatgatag tgtggtctca
     6301 ttgtacaaag tggtggtacg aaagccctgt aaaaggtcgt tgccatagtc ctgaacatgg
     6361 ctgtcattgt tgagaaagat gtcagcccca tagttcatgc ccgcactgtc tgcgccgcta
     6421 actgcggtga agaaaacatt actcagattc cattgtgcaa gtggctggta gttgacggtg
     6481 aagaggtgtt ctgcaaaatt ggcaccaagc gggtgcgcaa tggcctgtgc atagtgggta
     6541 taactgctgc cattgtcatt gctgtaagaa tacatatatg gtcttaccag attcatctcg
     6601 tactgaagat ccagaaaatc cagaccaaat gcattgatgt attgtaaacc tgtttgcaat
     6661 gcatatttgt ttccccacca tccggttgct ttggtaaggt caaagaaacg cagttcatcc
     6721 agaacgaact ggccgtagaa ggcgtattgt tgcagaaagg tccatttcca caacagcccc
     6781 aataaaacgt tgtcaggact acccaaaccc cattccactg aacgatagaa tatgagcggg
     6841 ttggcatagt tcaggtcaaa tccggtgggg tatccgaggc tgtcgttgcg ttgaaagacc
     6901 accgtttcaa agatgccaag atccaggttg ggcagtactt ccacattgag ctggtgcaga
     6961 gaagcatact ttttgcgcca gcggttgtca ggtaatttag gctggttcat gttgatgaga
     7021 gacaggaaat ttgcctggta tcgaatgcgc cagaactggg tttgcagccg gacataaaat
     7081 ggctctctgg cattgtcact ccataccagt gaccgcaccc cgtcgcccac gaacatctta
     7141 tcctgcccga cctgtaaaga caaatgttcg aacaactgca catccatgtg cccgcgtgaa
     7201 acaggaaagt caaagccctg cgccttaaag ggttttgacc agcccagcga cggaaccacc
     7261 ttttgcacat ttaggtttgc cgcatcggtt acataccccg gataagtggc ctgattatcg
     7321 gtggcaaaca cgtaaaagcc cacacggtca tcaataccgg cacgtacttc cagacctctg
     7381 gtgttttcaa agaccagctt acctgaatca cttgttcccc cgaaactatt gtagatgaca
     7441 ggattcagat tcacaaagta gtgatcgcct tctgtttgga agaaatttgc actttcctta
     7501 aaaaagatcc ccaacagagg cttatttcta cctgtctgaa aaccctccag aaactcagat
     7561 gcatcatccc gcagccagaa tgtattgaac acatcccggc tatcatatga ttccagggtg
     7621 ttggtgggct tgccataatt tttttgcatc caggagctga tgtccttcct gccataaggc
     7681 cgcatggaag tgtgaaggtc cagtgaagta tccagaatgg catagcgccg caacaacctg
     7741 tcgccgtatc cattcaacgg aatattggta ttttgtgcct gcagatcaca ggcagccaac
     7801 accgttacca gaaaaagaaa taaaatgaac cggccagctg gcatgtattt tggattatac
     7861 cacgcatagt taagaaaaaa cccccgatga gaaaacagtt attgtggata gtatttctgc
     7921 cgttgaccac cctggcacag catctgggtt tcaacggagc gggtttcttt gaaaattcca
     7981 acgaggaggt catacgctac atgcagtcct ggaccggaga tttcactatt cgggtaccgg
     8041 ggggtgccat cagtaagttt catgatccgt ataatgtcag acaggggtgg ggaatgaccg
     8101 atgaaagcgt tcagagctgg tttgcccgcg atggttttga cgaagacgga aatggactgg
     8161 acaagtggtt gaggaagaca ggtgaacagc ctgatcacag ttatctggat gatctcatag
     8221 ccctgcagaa aaagtttccg aaaatgcgtg tgctgtatgt gctgaatgta ttgaacagca
     8281 cacctgaagc caatatgcag gctatcagat acctggtgca aaatggggtg cgggttaccg
     8341 gtgtggaggc aggcaacgag atctatggca agtacagcag ttttgacgag tatgccagag
     8401 atttccaacc catttttgat ctgctggacc gtgagtttcc tgatatcagt aaaggattgg
     8461 tggcaggagc caatgttcaa cgtaaacagt tacagcagtg gaatgatgca ctcgctgcct
     8521 accggggcga ttatgatgcg gtcatactgc actactatta taccgcccgc gaactgggct
     8581 ctgcttatga tcagctggaa aaagtaaagt acactccggg aaaatactat cctgcacttg
     8641 atcaggcttt tcaggcggca aagttggcaa tgacgaagga agatatgatc ggccgtggaa
     8701 tacagtatgc caaagaacaa ttccccggca aaaagatctg gatcacagaa tggaatacca
     8761 aaccatcaga taaactgaat aatacattgc tcaatgcagg gtggcaatac gaagaactgc
     8821 ttcgtcaccg ttctgatgtg gaatacctgc tggtacataa tggagctggt cctgacaaat
     8881 acgccatgat gtctcgcaga caaagcaaat atgattcaca acctgaatac atgattcgca
     8941 gagctgcgtt ttacagcttt caactggctc tggaagcagc ttattccaag cctctggaac
     9001 gaggtaagaa tacttatata aatggcggac agcgcagcca gtactggttc agcaacctgg
     9061 aaaaggatac ctgggaacca gccatagtgt ggaataaaga ccagttcaaa gacgtgcaga
     9121 tccattttgt gcatgcacct tacttgtaca gcgccgccgg tgtggcaggt tatatgtcta
     9181 agggcagtac cccctcttat gagatagatg gtatcagcac catgcctttt acgggttcga
     9241 tacctgcata cagttacgga tatattgaat ggataccgag atagcgattg taacattttt
     9301 gcaggactga acgtcatacc tgtacctgct gatgatcgct gttttcaaaa ccggaaaata
     9361 tggcgatgca atttaaagac atccagcacc tttacctccg cggagggttt ggtgcaacgc
     9421 cttcccagat cgcttaccgc acaggtaagg aaagaacaga tctggttgaa gaactgtttg
     9481 cagcttcccg ttcttacagg gggctgaacc acctggagga tcctgtcaaa ggaaagaagg
     9541 taggtaacct gcgtatcctg ttcatgctgt tgcgttctca ggatcagatg cgtgaactga
     9601 atctggaatg gatcagactc atggccggaa cgaatgcagc tcttcgcgag aagatgacct
     9661 tcttctggca tgatcatttt gcaaccagta caccgcttgc ctggctcatg caggtgcaga
     9721 acaataccct gcgcaagcat gcattgggaa atttccgtac catgctgcac gccatttcaa
     9781 aagatcctgc catgatcgtg tacctgaaca atcagcagaa taaaaaagat caccccaatg
     9841 agaattttgc cagagaggtg atggagctgt tttctcttgg tatcggtcat tatacagaac
     9901 aggatgtcaa ggaagcagca cgggcattta ccggttggac ggtcaataaa aaaggtgagt
     9961 atgaatttga cgagaaagaa catgatgaag gagaaaagac cgtgctggga agaactggta
    10021 actggaatgg aaatgacatc attgatatgc tgctggaag
//
LOCUS       DAOMAX010000077        10010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_916_length_10010_cov_3.777901, whole genome shotgun sequence.
ACCESSION   DAOMAX010000077 DAOMAX010000000
VERSION     DAOMAX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_10010_cov_3.777901"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PK742_05730"
     CDS             <1..1825
                     /locus_tag="PK742_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76125.1"
                     /translation="QAYWVDLPDFAASVDQVQIQRGVGTSVNGVSAMGASINLTTNLL
                     EEKPSATLAWQTGSFGLQRISAGWNTGLLQDHWILEGRLSAIHSDGYVDRASADLRSF
                     FVTAAWKNRDFKSVLNVFSGLEETYQAWAGIPKDSLQTNPTYNPYTYSDQVDHYGQTH
                     VQWHQYWNPDNDRSLTLSFNLTSGAGYYEQLEVDQQLSDYGIMLPSVGDTILQTTDLI
                     TRKWLDNMMYGGLFKYSRNFKRSQLTAGGAVFRYDGLHFGEVIQASYLPLLDPAYRYY
                     ADSAYKTDANLYSQFLYQAGEKTHLWADIQFRTIHYAFTGLDSAGMPLPDAVTLPFVN
                     PKAGVRWKWTENVTSYLSVAIAGREPNRDDYVAAAGQLPKPEYLYDAEGGASYRKSNL
                     TLSANMYFMYYQDQLVLTGQLNEVGAYIRQNIPRSYRTGLELVWNTRLPAGFEWKGNL
                     TGSINRILAYTQYLDNWDEGGQSTRNYESTTIAFSPQWIAHQSLSWSSSRSWIAGLWV
                     SARWDSRFVSRQYLDNTEDPDASLDPYVVHDISAALTWKHKEKDQDLITLSFRVNNLL
                     DVLYVTNGWSYRYLSAGTEQVLTGYYPQAGRHFVTGLILHI"
     gene            1966..4563
                     /locus_tag="PK742_05735"
     CDS             1966..4563
                     /locus_tag="PK742_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU76126.1"
                     /translation="MNRRNLLIACGTALTLAAAVFFTMKPDHSESAAAADYFDPSYSA
                     VSWAQQAQLERSGLAQSWMDAVKAEINRRDYAFRQEGSDLYTASHAAQGLTFHISESG
                     FLVSGADWDAELNLDAVYAGSLPVDAGDLIAVDLDLNKLVYHYEVMDVEYINDAKGMR
                     QNFIVKDAERGDIRVEQQILTELETAIENNSLVLKQNGRNVYTYTDMKVWDNDGRILT
                     ASMHMPDSKQLALAVDASAATFPLTIDPVSRTYDWKVEGDNLNAGFGFWVAGNGDING
                     DGYDDVVIGAPEYTNTFAMEGAFFVYYGGPDGLPLTPDFVYYGGQEESGMGKCISIEG
                     DVNGDGYSDVLVGQHQYSPPSDQGRALLFYGGPSGISSTPGWTFTAGRKKAKLGEAVT
                     MAGDLNGDGYTDVCIGAHAWDNDEEMGDLGNKAGKFWVFHGGPSGLSTVPAMECVADV
                     TDANLGVSMDRAGDVNGDGYDDLHVGGYIFLVGDGMICTFHGGPGGADGTPDFMAVGG
                     AHDTSFYAVNLSAAGDVNGDGYDDVVIGAPRFDAGGTYQAGKLHLHLGGPSGLDTTIA
                     WIVQGTQYDERFAFNVNDAHDLNADGYDDVLVTSKYFIPEGSTDSVGKTMIYLGGPNG
                     PSLEPNWEFVGESTNGAGQNACLAGDVNGDGQEDIILSGSAYSGDLYHEGVVYGFYGL
                     PYKCDPPVDFVVSGVSPTTATFEWRWVFGSTTYKLFIKELGSTGAPVSLNLNGNDITV
                     TGLKPATKYQCYVKARCEDGYTNRSENIIISTPPMRTENNVIVEDPTIYPNPVRDRMN
                     IDFGTNEGSATVLVYDMSGTLMIEKHYDLSQSRTVELTDMNAVPDGVYLVTLDINNVR
                     TTKKITKTN"
     gene            4618..5025
                     /gene="arfB"
                     /locus_tag="PK742_05740"
     CDS             4618..5025
                     /gene="arfB"
                     /locus_tag="PK742_05740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQU76127.1"
                     /translation="MDTDQILKECTFTFSRSGGKGGQHVNKVSTRAEIWWDLETSAAI
                     SEEERKLLRIQLPARYVHGSLVHLSSSQSRSQGSNKEYVQHKLLEILRLALAPVKQRK
                     PTKKPAAANRKRLEEKARRSSAKESRNRRWETD"
     gene            complement(5022..6617)
                     /locus_tag="PK742_05745"
     CDS             complement(5022..6617)
                     /locus_tag="PK742_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQU76128.1"
                     /translation="MSLSSEIQRRRTFAIISHPDAGKTTLTEKFLLYGGAIQVAGAVK
                     SNKIKKSATSDFMDIERQRGISVATSVMTFEYDSTLINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVVDCVKGVEEQTEKLMQVCRMRAIPVIIFINKMDRDGKDPFDLLDELEEKLQ
                     IKVRPLSWPVNMGSHFKGVYSLYHKHLNLFTPGSQRLDADILTISDLQDPTLDDKVGN
                     KDAEQLRHDVELIEGVYEPFSLTTYREAHIAPVFFGSAINNFGIQELLETFIEIAPVP
                     QHRITEERDVRPDETPFSGFVFKIHANIDPRHRDRIAFLRVVSGTFYRNTVYKHVRLD
                     KNLRFNNPYTFMAASKDVIEEAYPGDVIGLYDTGNFKIGDSLTEGEALHYKGIPSFSP
                     ELFMELINADPMKSKQLDKGVVQLTDEGVAQLFIHSSGNKKVVGTVGQLQFDVIQYRL
                     ENEYGAKCRFTPIQMHKACWITSDDKKKLDEFMRLKSEFMYFDKEKNPVYMAETSWAL
                     NVAIENNPDIAFHTTTEFMSAVS"
     gene            6682..7155
                     /locus_tag="PK742_05750"
     CDS             6682..7155
                     /locus_tag="PK742_05750"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQU76129.1"
                     /translation="MKKGMTDYLLVAMQFALLGGILLMDGLLITTPDPVLKWAGALIL
                     FTGILTGIVAVIQMNRYLTVFPTPKKGARLLSSGVYSWIRHPMYTALILAGSGYALWS
                     GSLLRLVLTIILLILFEYKSQYEEVRLSKFFDEYESYQKTTGKFIPRLQKWKEIL"
     gene            7152..7925
                     /locus_tag="PK742_05755"
     CDS             7152..7925
                     /locus_tag="PK742_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQU76130.1"
                     /translation="MKNHIIHHKPTILFLTLGVFFVVNALIAEFIGVKIFALEPSLGL
                     NTFRWKLFGIEGSLNFTVGVLLWPVVFIMTDIINEYFGMKGVRRLSYMAVIFILYAFA
                     AVFIAIRLAPADFWPGSYVNQGVPDMQAAYAAIFGQGMWIIAGSVVAFLIGQLVDVFF
                     FHRIKAITGEKRIWLRATGSTAISQVFDSVVVLYIAFVLGPAHWSVSMWLAIALVNYC
                     YKLLAAVVLTPLLYLVHHWIDNFLGAELSQQLRREAMSE"
     gene            complement(7926..8654)
                     /locus_tag="PK742_05760"
     CDS             complement(7926..8654)
                     /locus_tag="PK742_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQU76131.1"
                     /translation="MRSILPTIIIAAGVVLTGMILGNAFKNRYDYQQTIAVTGLGKTD
                     FESDLVVWSGYFQKKNPDLKEAYAQLNEDRDLIKSYLKEKGIPDNEIKFSSVDINRDY
                     DSYYDTNGKYVSRFAGYSLNQYVTIESHQLDEIDALSREVTELINKGVEFYSNQPSYY
                     FTRLDSLKIDLIARATEDGRKRAENIAEQAHARLGSLTNASMGVFQIIAQNSTEDYSW
                     GGTFNTSSRFKTANITVKLEFESK"
     gene            complement(8680..9693)
                     /locus_tag="PK742_05765"
     CDS             complement(8680..9693)
                     /locus_tag="PK742_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76132.1"
                     /translation="MGRALIIIPLLTSLLHLTQAQNVALQIQEHTLQTSFRGIACGDG
                     GQVWTSGENGTIGHSVDGGVTWQFIVVPGMENASFRDIEWCDDGSVVVMSATQPAAMI
                     RSADGGQHFDITFSKMDSTWFLDGFDIHRNGWGLCYGDPINGRMTLLETKDFGKSWTV
                     LPTGPPVDPGTAAFAASGSGLHILSARKAVFITGGTASDLYQTKDRGRSWQKVSSTGL
                     PSGPTSGAYGMAVSGKKIYVVGGDYTNENDTTGVFVFSENGGKTWKKPVVSPSGYRSG
                     IAIGKNLIIVCGPTGVDYTNIQGGLVFKHWSDTAMHTLTNCEGVFYIAGPDGRTGHAF
                     DTK"
     gene            complement(9678..>10010)
                     /locus_tag="PK742_05770"
     CDS             complement(9678..>10010)
                     /locus_tag="PK742_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76133.1"
                     /translation="ALENLQNDTTIAGLHCTEYLVIRSGYTRYGTDTRVWITNDLHVQ
                     PQRFDIETDVNRATTPLPLYLGASEGLVVRMQYETDGLMITYDLSAWDSTLTNTDIFT
                     LPEGWDVH"
BASE COUNT         2544 a         2325 c         2419 g         2722 t
ORIGIN      
        1 acaggcctac tgggtagatc tgcccgattt tgcggcatct gttgatcagg ttcagatcca
       61 acgcggtgtg ggcacttccg taaacggtgt cagcgcaatg ggagcatcca tcaacctgac
      121 caccaacttg ctggaagaaa agccatcagc taccctggca tggcagaccg gtagtttcgg
      181 attacaacgg atatcagccg gatggaatac tggtctgctg caggatcact ggatactgga
      241 aggcagattg tctgccatac actcagacgg ttatgtggac agggcttctg cagatctgcg
      301 gtctttcttt gttaccgctg catggaagaa ccgagacttc aaatcagttc tgaacgtctt
      361 ttccggattg gaagaaacct accaggcatg ggcaggtatt ccgaaagatt cgttgcaaac
      421 aaacccgacc tataatccat atacctatag tgatcaggtt gatcactatg gtcaaaccca
      481 tgtacaatgg caccagtact ggaaccctga caacgacagg tcactgactc tttcttttaa
      541 tctgacctcc ggtgccggct attacgaaca actggaagta gatcagcagc tgtcagatta
      601 tggtatcatg ctgccatcag tgggcgacac catcctgcaa acaacagacc tcattacacg
      661 caaatggctg gacaatatga tgtatggcgg attgttcaaa tactccagaa actttaaacg
      721 cagccagctt accgcaggag gtgcagtgtt tcgctatgac ggcctgcatt tcggcgaggt
      781 cattcaggct tcttatctgc cattgctgga tcctgcctac agatactatg ctgattcagc
      841 ctataaaaca gatgccaacc tgtattccca gttcctctac caggccggag aaaaaacaca
      901 tttgtgggca gatattcaat tcagaacgat ccactacgct tttacaggat tagattctgc
      961 aggtatgccg ttgccggatg ccgttaccct gccttttgta aacccgaaag ccggggttag
     1021 atggaagtgg acagaaaatg tgaccagcta cctgtcagtg gctatagccg gcagagagcc
     1081 taacagagat gattatgtgg ctgctgctgg tcagcttccc aagcctgagt atctgtatga
     1141 tgcagaaggc ggagcatctt acagaaaaag taacctgact ttatcggcca atatgtattt
     1201 catgtattat caggatcagt tggtccttac aggacagttg aatgaagttg gtgcttatat
     1261 caggcagaat attccacggt cttaccggac tggcctcgaa ctggtctgga ataccagatt
     1321 acccgctggt tttgaatgga aaggaaatct gaccggcagc atcaatcgca tcctggcata
     1381 tacgcaatac cttgacaatt gggatgaggg tgggcaaagc acccgcaact atgaaagcac
     1441 taccatcgct ttttctccac agtggatagc tcatcaatca ctctcatgga gctcgtcacg
     1501 ttcatggatt gcaggattat gggtttctgc cagatgggat agccgctttg tatcacgtca
     1561 gtacctggac aatacagaag atccggatgc cagtcttgac ccctatgttg ttcacgatat
     1621 ctccgccgca ctgacgtgga agcacaaaga aaaagaccag gatctgatca ccctcagctt
     1681 ccgggtgaac aatttactgg atgtattgta tgttaccaat ggatggtcgt acagatacct
     1741 gagtgccgga acagaacagg tgcttactgg ttattatccg caggcaggga gacattttgt
     1801 gacaggatta attttgcaca tttagttttc aacctgctga tattgacaaa aatcaatcaa
     1861 aaaatgcttt tacaatactt ttaagttcct aaatttgttg tcagcatacc caatgaatag
     1921 agcaacgtgt atcctgaatc aattaaaccc gccaaaaaac cagtcatgaa cagacgtaac
     1981 ctgttgattg catgcggaac agcactgacc cttgctgctg ccgtattttt taccatgaag
     2041 cccgatcact ctgagagtgc agcggccgct gattatttcg acccctctta ttctgccgtt
     2101 tcatgggcac agcaggctca gttggaaagg tccggacttg ctcagtcctg gatggacgca
     2161 gttaaggcag agatcaaccg tcgggattat gctttccgtc aggaaggttc agacctctat
     2221 accgccagcc atgcagcgca gggacttacc tttcatatat ctgaatcagg tttcctggta
     2281 tcaggcgctg actgggatgc tgaactgaac ctggacgctg tctatgcagg cagcctgccg
     2341 gttgatgccg gcgatctgat cgctgtagat ctggatctta ataagctggt ttatcactat
     2401 gaagtaatgg atgtagaata catcaatgat gcgaaaggta tgcgccagaa cttcatcgtt
     2461 aaggatgctg aacgtggcga tatcagagtg gaacagcaaa ttctgaccga actcgaaacg
     2521 gctattgaaa acaacagcct ggtgctgaaa caaaatggcc gtaacgtata tacttatact
     2581 gacatgaaag tatgggacaa tgacggacgc atcctgaccg cttccatgca catgccagac
     2641 agcaagcaac tggctctggc tgttgatgcc tctgctgcta ccttcccgct taccattgac
     2701 cctgtttcca gaacatatga ctggaaagta gaaggcgata acctgaatgc tggttttggt
     2761 ttctgggttg ccggtaacgg cgatattaac ggcgatgggt atgatgatgt agtgattggc
     2821 gctcctgaat acacgaatac cttcgcaatg gaaggtgctt tctttgtata ttatggtggt
     2881 cctgatggcc ttccgcttac tcctgacttt gtttactatg gcggtcagga agaatcagga
     2941 atgggtaaat gtatctcgat tgaaggtgac gtgaacggcg atggttattc cgatgtactg
     3001 gtcggtcagc atcagtacag tcccccgtca gatcagggac gtgcgctgct gttctatggt
     3061 ggtccttctg gaatcagctc cacaccaggc tggacattca ccgctggtcg taaaaaagcc
     3121 aaattgggtg aagcagttac catggccggt gatcttaatg gcgatggtta tacagacgtt
     3181 tgtatcggtg cccacgcctg ggataacgac gaagaaatgg gcgatctggg taacaaggca
     3241 ggtaaattct gggtgttcca cggcggccct tcaggtctgt caacagttcc tgctatggaa
     3301 tgtgtggccg atgtaacaga tgctaacctg ggtgtttcta tggaccgtgc cggcgatgtg
     3361 aatggcgatg gttatgatga cctccacgtg ggtggttata tattcctggt tggcgatggt
     3421 atgatctgta ctttccacgg tggtcccggt ggtgccgacg gtactccgga tttcatggct
     3481 gttggtggag ctcatgatac ttcattttac gctgtaaacc tgtcagcagc aggcgatgtg
     3541 aatggcgatg gttacgatga tgtagtgatc ggtgctcccc gctttgatgc cggcggaacc
     3601 tatcaggctg gtaaattgca cctgcacctg ggtggacctt ccggtctgga taccactatt
     3661 gcatggatcg ttcagggtac tcagtatgat gaacgatttg cattcaatgt gaacgatgcg
     3721 catgacctga atgccgatgg ttatgacgac gttcttgtta cctcaaaata ttttatacct
     3781 gaaggcagta cagactctgt tggtaagacc atgatctacc tgggtggtcc gaatggtcct
     3841 tccctggagc ctaactggga gttcgttgga gaatcaacca atggtgccgg acagaacgcc
     3901 tgtcttgccg gcgatgtcaa tggtgacggt caggaagata tcatcttgtc aggaagtgcc
     3961 tatagcggcg acctctacca tgaaggtgtg gtatatggtt tctatggtct tccctataag
     4021 tgtgatccac ccgttgactt tgtagttagc ggtgtatctc ccacaacagc cacttttgaa
     4081 tggcgctggg tattcggctc tactacttat aaactgttca tcaaggaatt gggcagcact
     4141 ggtgcaccag tatctctgaa cctgaatggt aatgatataa cggtaaccgg tctgaagcct
     4201 gctaccaaat atcagtgcta tgtaaaagcc cgttgcgaag atggctacac caaccgttct
     4261 gagaatatca tcatcagcac tcctcccatg cgcacagaga ataacgtgat cgtagaagat
     4321 cctaccatct atccgaatcc tgttcgcgac agaatgaata ttgattttgg aaccaatgaa
     4381 ggaagtgcta ccgttctggt ctatgacatg tcaggaacac tgatgattga aaaacattac
     4441 gacctgagtc agtctcgtac cgttgaactg actgatatga acgcagtacc tgatggtgtt
     4501 tacctggtta cactggacat caacaatgtt cgcacaacca agaagatcac caagacgaac
     4561 tgatccaaaa cctatcatca tattcagata cctccccaag ggaggtatct tttttttatg
     4621 gatacagatc aaatactaaa ggaatgcacc tttaccttca gcagaagcgg aggtaagggt
     4681 ggtcagcacg tcaataaggt ttctaccagg gctgagatat ggtgggatct ggaaacgtct
     4741 gcagccatat cggaagaaga aaggaagctg ctgagaattc agcttcctgc acgatacgta
     4801 cacggtagcc tggtccatct aagctcttca cagtcacgct cacagggaag taacaaagag
     4861 tatgtgcaac ataaactact ggagatactc cgcctggcat tagcacctgt aaagcagaga
     4921 aaaccaacca aaaagcctgc tgctgcaaac agaaaaaggc tggaggagaa agcccgccgt
     4981 agttccgcta aagagtccag aaacaggcga tgggaaactg attagctcac tgcagacata
     5041 aattccgtgg tcgtgtggaa agcgatgtca ggattgttct ctatggccac attcagcgcc
     5101 cagctggttt cagccatata aaccgggttc ttttccttat cgaaatacat gaattcgctt
     5161 ttaagcctca tgaattcatc cagtttcttc ttgtcatctg aggtgatcca gcaggccttg
     5221 tgcatctgaa taggagtaaa cctgcacttg gctccgtact cattttccag tctgtactgg
     5281 atcacatcga actgaagctg gcctactgtt ccaacaactt tcttattgcc gcttgaatga
     5341 atgaacagct gcgctacacc ttcgtctgtt agctgaacaa cacccttgtc aagctgctta
     5401 gacttcatag gatctgcatt gatcaattcc atgaacagtt ccggagagaa gcttgggata
     5461 cccttgtagt gaagtgcttc gccttcagta agagaatcgc caatcttaaa gttcccggta
     5521 tcatacagac cgatcacatc gccgggatag gcttcttcaa tgacatcctt actggccgcc
     5581 ataaaggtgt acggattatt gaagcgcaga ttcttatcca gccggacgtg tttatacacc
     5641 gtattgcgat aaaaggtgcc ggacaccacc cgcaggaagg caatgcggtc gcggtgtcgc
     5701 ggatcaatat ttgcatggat cttaaagacg aagccggaga agggtgtttc atctggcctg
     5761 acatctcttt cttctgttat ccggtgctga ggaaccggtg cgatctcaat gaaggtttcc
     5821 agcaattcct gaataccgaa gttgttgata gcactgccaa agaagaccgg agctatgtgc
     5881 gcctctctgt aggttgtaag cgaaaacggc tcatagacgc cttcaatcag ttccacatca
     5941 tgtcgcaact gctcagcatc tttgttgccc actttgtcgt cgagtgtagg atcctgcaga
     6001 tctgatatgg tcaggatatc agcatccagt cgctgagaac caggagtaaa aagattcagg
     6061 tgtttgtggt ataatgaata gactccctta aaatgagaac ccatgttgac tggccaggag
     6121 aggggtctta ctttgatctg cagcttttct tccagttcat ccagcagatc gaacgggtct
     6181 ttaccatcac ggtccatctt attgatgaaa atgatgaccg gaatagcccg catacgacaa
     6241 acctgcatca gtttttctgt ctgctcttcc acacccttaa cacagtccac caccaatatg
     6301 acagaatcta cagcagttag tgtgcgatag gtgtcttctg caaaatcttt gtgccccggt
     6361 gtatccagaa ggttgatcag agtactgtca tactcaaagg tcatgacact ggtagcaaca
     6421 gagattccgc gttgcctttc aatgtccatg aaatctgagg ttgcactttt tttgatcttg
     6481 ttacttttta ccgcacctgc cacctggata gcaccaccat acagcagaaa tttctctgta
     6541 agagtggtct ttccggcatc cgggtgactg ataatggcaa aagttcttcg cctttggatc
     6601 tcgctgctca gactcatcag ggcgcaaaat tacacaaatg gtacggcgtg tatcaggact
     6661 cattcagtag atttatggct gatgaaaaaa ggaatgactg attatctgct ggtggctatg
     6721 caatttgccc tgctgggtgg catactgctg atggacgggc tgttgatcac aacaccggat
     6781 ccggtcttga aatgggcagg ggcgctcatc ctgttcactg gaatactgac aggtattgtg
     6841 gcagtcattc agatgaacag gtacctgacc gtatttccaa caccaaaaaa gggggcacgt
     6901 ttgctttctt ccggcgtgta tagttggatc cgtcatccca tgtacactgc actgatcctt
     6961 gcaggatcag gttatgcgtt gtggagcgga agcctgctgc gactggtgtt gacaattatt
     7021 ctactgattc tgttcgagta taagtcgcag tatgaagagg tcaggctttc taagttcttt
     7081 gatgagtatg aatcctatca gaagaccacg gggaaattca ttccaagact gcaaaaatgg
     7141 aaagaaatat tatgaaaaat catatcatac atcacaagcc caccattctg tttttaaccc
     7201 tgggggtctt ttttgttgtc aatgcactga tcgctgaatt tatcggggtg aagatctttg
     7261 ccctggaacc cagccttgga ctgaacacat tcagatggaa attattcggc attgaaggaa
     7321 gtctcaactt cacggtagga gtcttactat ggccagtggt tttcatcatg accgacatca
     7381 tcaatgaata ttttggtatg aaaggggtgc gtaggctatc ttatatggct gtgatcttta
     7441 ttttatatgc atttgcggcg gtgttcattg ctatcagact ggcacctgca gatttctggc
     7501 cggggtccta tgtaaaccag ggtgtacctg acatgcaggc agcctatgca gccatttttg
     7561 gccagggaat gtggatcatt gcgggatcgg ttgttgcatt tctgatcggt cagctggtag
     7621 atgttttttt ctttcacaga ataaaggcga tcaccggaga aaagcgaatc tggctgcggg
     7681 ctaccggttc aactgccatt tcgcaggtgt ttgacagcgt agtagtatta tatattgcat
     7741 ttgttctggg gccggctcac tggtcagtca gcatgtggct tgccatagct ttggtcaatt
     7801 attgctacaa gctgcttgcg gctgttgtgc ttacgccact tctgtacctg gtacaccact
     7861 ggatagacaa ctttctgggt gctgaactga gtcagcaact tcgccgggaa gcgatgtcag
     7921 aatgatcatt tactttcaaa ctccagtttg accgtgatgt tggcggtctt aaatctggag
     7981 ctggtattga aagtgcctcc ccaggagtag tcttcggtgg agttctgcgc tatgatctga
     8041 aagacaccca tgctggcgtt ggtaagagaa cccaatctgg catgggcctg ttctgcaatg
     8101 ttctctgcac gttttcttcc atcctcagtt gcccgggcaa tcagatcgat cttcagtgag
     8161 tccagtctgg taaagtagta agaaggttga ttgctgtaaa attccacccc cttgttgatc
     8221 agttcagtca cttcacgtga cagggcatcg atctcatcca attgatgtga ttctatggtc
     8281 acatactgat tcagactata gcctgcaaac ctggatacat acttgccatt ggtatcatag
     8341 taactatcat agtcgcgatt gatgtcaact gaagagaatt tgatctcgtt atcaggaatg
     8401 cctttctctt tcaggtagct tttgatcaga tctcggtctt catttaattg tgcataggct
     8461 tctttaagat ccgggttctt tttctgaaaa taaccggacc ataccaccag gtcgctttca
     8521 aaatctgttt tacccaaacc tgtcactgca atggtttgtt gatagtcata acggttcttg
     8581 aatgcgtttc caagtatcat accggtcagt acaactccgg cagcgatgat gatggttggg
     8641 agtatcgatc tcatgggtgt aattaatgtt gtaagttatt cattttgtat caaatgcatg
     8701 acctgttctt ccgtccggtc ctgctatata aaatacacct tcacagttgg tcaatgtatg
     8761 catggcagta tcagaccaat gtttaaaaac gaggccgcct tgaatgttag tataatccac
     8821 tccggtgggt ccgcaaacta tgatcaggtt cttaccaatg gcaattcctg accggtagcc
     8881 tgacggagaa acaactggct ttttccaggt ctttcctcca ttctcagaga atacaaaaac
     8941 acctgtagta tcattttcgt tggtataatc gccgccaacc acgtatatct tttttccaga
     9001 cacagccatt ccgtaagcac cagaggttgg tccggatggc aatccggtag aagacacttt
     9061 ttgccaggac cggccgcgat cttttgtctg gtaaagatct gaagctgtgc cccctgtaat
     9121 gaatactgct tttcttgctg acaggatgtg caggccgctt ccgcttgccg caaaagcagc
     9181 tgttcccgga tctaccggag ggcctgttgg caaaacagtc caggacttcc caaaatcttt
     9241 ggtttcgagc aaggtcattc tgccgttgat ggggtcgccg taacaaaggc cccagccatt
     9301 cctgtgtata tcaaaaccat ctaaaaacca ggtgctgtcc attttgctga aggtgatgtc
     9361 aaaatgttgt ccgccatcag ctgaccggat catagcagca ggctgagtag cactcatcac
     9421 cacgacagaa ccatcgtcac accattctat gtctcgaaat gatgcgtttt ccattcccgg
     9481 aacaacgatg aactgccagg ttacaccacc atccaccgaa tgtcctatgg ttccattttc
     9541 accactcgtc catacttgtc cgccatcgcc gcaggcaatt cccctgaagc tggtctgaag
     9601 ggtgtgttcc tgtatttgca gtgccacatt ctgagcctga gtcaaatgca gcaggctggt
     9661 taaaagcggt atgatgatca gtgcacgtcc catccttcgg gtaaggtaaa tatgtctgta
     9721 ttggtaaggg tactgtccca tgccgacaga tcataggtta tcatcaggcc gtcggtttca
     9781 tattgcatcc tgaccaccag tccctcggaa gcccccaggt agaggggtag gggtgtggta
     9841 gccctgttca catcagtttc aatgtcaaat ctttgaggct gaacatgcag gtcgtttgtg
     9901 atccagaccc ttgtatcagt accatagcgg gtatagccgc ttcttattac caggtattca
     9961 gtacagtgca gccctgcaat ggtggtatca ttctgaagat tttccagagc
//
LOCUS       DAOMAX010000078         9925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_927_length_9925_cov_4.660993, whole genome shotgun sequence.
ACCESSION   DAOMAX010000078 DAOMAX010000000
VERSION     DAOMAX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_9925_cov_4.660993"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..2350
                     /locus_tag="PK742_05775"
     CDS             134..2350
                     /locus_tag="PK742_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQU76134.1"
                     /translation="MKRVYLFLFFISVFLRVQAQLITIAEARASAEGTTVTVRGIVVN
                     GSELGPIRYMQDETGAIAAYDFAATADVVAGDSIEVTGVLDNYNSLLEISPVTSLTIL
                     SSGNPVPAPLPVTLTDGWVEEMEGYLVEVSGVHFTETGTFSTAGSGTNYDVTDGISSA
                     QVRIQTSTNIDGTVIPEEDLFITGIMSQYAPGGSGGYQLLPRDLTDISTGGNPPVISS
                     GLSQTNITTTGFTVGFETLYNGNTIIYYGTSAGALTSTSEDAAMTTSHALGLAGLTPA
                     TIYYVQAASVSASNDTSWSAVAAMATSSNSSGEIRVWFNNPVDNSVSTGVNAMYDTHF
                     ADTIISYIDNAVYSIDMALYNIDNINNIVTALNDAYVRGVTVRVICDAGVNSAAYDLL
                     SIGAGNKKKSPSGMTPDGDYYGIMHNKFLVIDVVSTDADQPWVIGGSTNFTDEQLKID
                     KQNMIAIQDHTLAKAYRAEFEEMWSGSFGPEKTNNTPHDFIIDGKHIELYFSPSDNTE
                     SHILSSIQSADHDLYFGVFSYTRYGISYEIQDAIDRGVFAAGIWDQTDPDDPTAIEVL
                     ESAMGSTLFEASGSPLFHHKYLIVDPNCPQSDPLVLTGSHNWTSSANSRNDENTLIIH
                     DSTIANIYYQEFSQRYQDEGGTEVVGDVCDYVAVDAVQAVPLQVYPNPANNWIVLESD
                     QEGKVSIFDLSGNMLNTFQITEGTNTIPVIALQTGMYVMLLQTEGAVFTGMISIQH"
     gene            complement(2351..3787)
                     /locus_tag="PK742_05780"
     CDS             complement(2351..3787)
                     /locus_tag="PK742_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQU76135.1"
                     /translation="MIKGIVKASALFTMGNMMPLLTSVVLLFPYTENLSTALYGELAL
                     YIAFTIFIQFTANYGMDNYVGIQHFRYKKDPEQMKAFVGSVVGALLVIGIVLITVFLL
                     LGSFIFQMVFHGELDFFPFGFMCVITGVCNAFFRTYINFLYYRDKPVRHVVFNLFNFV
                     VTIAVCTIGVYRYPEALTGPIWGRFLSGIAIFLLSAVFFQREYGIRFNRSYLEGLHKF
                     CFPVYIYVILGWVVFYINSFIINYYNTTSDVGIYDFALKVVLLIDVTQTAVSATINPR
                     IYQIWTDTGLQKSTTGENRFHHVFTMFSVIMIAVSILALPLLVQLFVRNESYYAIFPF
                     IPVLAVSFAFRVLANTYYNPLMYFKKTGGLPRAYGYSSLVQIVSCVAFLQWFGLEGAF
                     WSFFLSKVAVVFFSWMESRKIFRFEFNNYKMFLLPILYVLTTMTIFYLIPQNQYFLAA
                     SLQLAVAGILVLLFFRKDLGAYRLLLNR"
     gene            complement(3897..4226)
                     /gene="ssb"
                     /locus_tag="PK742_05785"
     CDS             complement(3897..4226)
                     /gene="ssb"
                     /locus_tag="PK742_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQU76136.1"
                     /translation="MRNKVQLIGHLGADPEIIEMGNDRKLAKINLATNESYKNANGER
                     VTDTQWHQVVAWGKLAGLAEKLLVKGSEVAVEGKLIHKSYTDKQGVKRYTSEVQISDL
                     ILLKKKD"
     gene            4451..5242
                     /locus_tag="PK742_05790"
     CDS             4451..5242
                     /locus_tag="PK742_05790"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU76137.1"
                     /translation="MFWSSLPPQKAVFIVGYWNSGTTLLTDILRKHPDLHLARGRRLP
                     NLEERSIEKLFLKAGTPFHQFGDYSEVLAHGFENYHSPHWNKDQTADFLRAFHRKFRT
                     PKGKDLLLKNPWLFFYQEWIKETFTGYPVRKIMILRNGYSQVVSKDYWLRTEGEPASQ
                     LKARAIFWHKAMDRYFQTWHNDPDCLTIRYENLCSDPMAIVRQVCLHLNIDPNPLTPH
                     LPDAFENRLTRWHQLEDGLKEMVHAEISAVQDRMDKIFPLRNPLT"
     gene            5309..5668
                     /locus_tag="PK742_05795"
     CDS             5309..5668
                     /locus_tag="PK742_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76138.1"
                     /translation="MTLKRCQECEREILGRTDKKYCSDMCRNNYNNRERSKEHQIVRQ
                     INCMLLRNRKILSRLLTSKVSKISRNKLMEQGFHFGYCTSTYTTRKGETYLFCYEYGF
                     LELADGQFCLVKRAEQY"
     gene            complement(5665..6561)
                     /locus_tag="PK742_05800"
     CDS             complement(5665..6561)
                     /locus_tag="PK742_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQU76139.1"
                     /translation="MNTYICTLLIGTTMLLSTTLHATTLEKSYFWEFEGKRYNLRYSF
                     DADDYKYYKQIKRDYNDFTVYMKETPGRNVADDFALELNRMARQYKLNQKETVEFVAS
                     FVQHFRYRDDGKYEYPRYPAETLVEQGGDCEDTAILLAALLRSMGYETILLSPEGHMG
                     VGLAVDGDLDGTGVEYQGEKYYYIETTNTGWGIGDYPDHLSTAIRILDPGSRVGCKPL
                     ALRAPGVKPSQANKDQATSSQASVGQHTAPQSGFQTDYTANKELVADDRGVLSTDTMV
                     IDGQKQETVTYQSSGRIAMSDH"
     gene            6702..7208
                     /locus_tag="PK742_05805"
     CDS             6702..7208
                     /locus_tag="PK742_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU76140.1"
                     /translation="MGATYIIETIRCRLRPLEPDDAEKMYALNLDPEVIRFTGDASFS
                     SVDEARTFLKAYDQYKKYGIGRWVVERKEDKEVIGWCGLKFHPDDDLYDIGYRFFRKY
                     WGQGYATETAGACLRYGREQLGLAEVYAHAMTGNIASWKVLEKLGMKRMEEIMMDGEP
                     AYLYKVQF"
     gene            complement(7205..8749)
                     /locus_tag="PK742_05810"
     CDS             complement(7205..8749)
                     /locus_tag="PK742_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQU76141.1"
                     /translation="MLVKTFASAVHGVQAHTITIEVNMTSGGTEMKSFIVGLPDNAVK
                     EAWQRIETAISHNGFRIPRKKYVFNLAPADIRKEGSGYDLPMAIGLLACTGNIPDTHL
                     KDYLIMGELSLDGQIQPVKGALPIAIQARKEGFKSFILPEQNAREAAIVDNLDVLGVT
                     HLNQVVDHLMDIKKIEPVHINTRDEFMHHLGQHDVDFSDVKGQENIKRALEIAAAGGH
                     NVILIGPPGAGKTMLAKRMPTILPPLTLHEALETTKIHSVAGKLGHGASLMYNRPFRS
                     PHHTVSDVALVGGGNNPQPGEISLAHNGVLFLDELPEFRRNALEVLRQPMEERKVTIS
                     RARFSVDYPANFMLIASMNPCPCGYYNHPEKDCVCGPGVVQRYLNKISGPLLDRIDLH
                     VEVTPVAIEELTAVKEEEKSASIRDRVIRAREIQEHRYASGSYGSNALLPSSAVREWC
                     RIDQPGQMLLNKAMERLGLSARAYDRILKVARTIADLADASDIRIEHLAEAIQFRSLD
                     RENWAG"
     gene            complement(8854..8964)
                     /gene="rrf"
                     /locus_tag="PK742_05815"
     rRNA            complement(8854..8964)
                     /gene="rrf"
                     /locus_tag="PK742_05815"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9033..>9925)
                     /locus_tag="PK742_05820"
     rRNA            complement(9033..>9925)
                     /locus_tag="PK742_05820"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2694 a         2333 c         2276 g         2622 t
ORIGIN      
        1 ttcagaacag atgccagccc cagccggcca atactttttc ctttacaaaa aaataaccac
       61 cggtaagcac cggtcatgct tcccccaatg gtcgtatagg tatctttgca cacttaaaat
      121 ccatccaata catatgaaac gcgtctatct ctttttgttt ttcatttctg tattcctccg
      181 ggttcaggca caattgatca ccattgcaga agccagagca agtgctgagg gtacgaccgt
      241 tactgttcgg ggtatcgttg ttaatggttc tgagctgggc cccattcggt atatgcagga
      301 tgaaacgggt gcaattgcag cctatgattt tgcagcaaca gcagatgtgg tagcgggcga
      361 ttccatcgag gttacaggtg tattggataa ttacaactca ttgctggaaa tcagtcctgt
      421 aacatcactg accatactca gctccgggaa tccggttcct gctccactac ctgttacact
      481 gacagatgga tgggtggaag aaatggaagg ttatctggtg gaagtgagtg gtgttcattt
      541 taccgaaaca ggcacttttt caaccgccgg atcaggcacc aattatgacg taacggacgg
      601 tatcagttcc gcacaggtca gaattcagac aagtaccaac atagacggaa cggtcatacc
      661 ggaagaagat cttttcataa caggcatcat gagccagtat gcaccaggtg gcagtggcgg
      721 atatcagctg cttccccggg atctgacaga catctcaacc ggaggaaacc ctcctgtcat
      781 ttcttccggt ttatcacaga ccaatatcac tacaactggt tttacggtag gttttgaaac
      841 attatataat ggcaatacca ttatttatta tggaaccagt gcaggtgccc tgaccagtac
      901 atctgaagat gctgccatga ccaccagcca tgcacttgga cttgcaggtc ttacccctgc
      961 caccatctac tatgtacagg cagcatcagt cagtgcctcc aatgatacca gctggtctgc
     1021 ggtagcggct atggctacat catctaacag ttcaggtgag atcagggtct ggttcaataa
     1081 ccccgttgat aacagtgtga gcaccggtgt caatgccatg tatgacacac attttgctga
     1141 tacgatcatt tcttacattg ataatgctgt gtattccatt gacatggctt tgtacaacat
     1201 tgacaacatc aacaatatcg ttactgcact gaacgatgca tatgtgcgtg gtgtaacagt
     1261 gcgggtcatt tgcgatgcag gggtcaacag tgctgcatat gacctgctaa gtataggggc
     1321 cggaaacaag aagaaatctc catcaggaat gacaccagac ggcgattact atggcatcat
     1381 gcacaacaag tttctggtca tagatgtggt ttctacagac gctgatcagc cgtgggtgat
     1441 aggcggttct accaacttca cagatgaaca gctgaagata gacaagcaaa acatgatcgc
     1501 cattcaggat cacactttag cgaaagcata cagggcagaa tttgaagaga tgtggagtgg
     1561 ctcattcggc cctgaaaaaa ccaacaacac gccgcatgat tttataatag atggaaagca
     1621 cattgaactg tatttcagtc cgagtgacaa tacagagtct catattctgt cttcgataca
     1681 aagtgcagac catgatctgt attttggtgt tttctcctac acgcgttatg gtatcagcta
     1741 tgaaattcag gatgctatag accgtggagt atttgctgca ggcatctggg atcaaacaga
     1801 tcctgatgat ccaacggcca tagaagtact ggagtcagct atgggatcca cattgtttga
     1861 ggcatcagga tcaccgcttt tccatcataa atatctgatc gttgacccga actgtccgca
     1921 atcagatccg ctggtactga ccggttctca caactggacc agcagtgcca acagcaggaa
     1981 cgatgaaaac accctgatca tacatgacag cactattgcc aatatctact accaggaatt
     2041 cagccagcgt tatcaggatg agggaggaac agaagtggta ggtgatgtgt gtgactatgt
     2101 tgccgtggat gcggtacaag ctgttcctct gcaggtatat ccgaatccgg caaataactg
     2161 gatcgtgctt gaatctgatc aggaaggaaa ggtgagcatc tttgatcttt ccggtaatat
     2221 gctgaatacc ttccaaatta ctgagggcac caataccata ccggtaattg ctttacaaac
     2281 cggcatgtat gtgatgttac tgcaaaccga aggtgcggta tttacaggta tgatcagtat
     2341 tcagcactga tcagcggttc aacaacagcc tgtacgctcc aaggtccttt cggaagaaaa
     2401 gcaggaccag aattcctgct acagccagtt gcagcgatgc tgccaggaaa tactgatttt
     2461 gcggaatcag gtagaaaatt gtcatggtgg tcaacacata caggatcgga agcaggaaca
     2521 tcttgtagtt attaaattca aaacggaata tcttcctgct ttccatccag gaaaagaaaa
     2581 ctacggccac cttagacagg aaaaaactcc agaaggcccc ttctaatcca aaccactgca
     2641 ggaaggccac acagctgaca atctgcacca gtgaactgta tccgtatgca cgaggcaacc
     2701 ctcctgtctt tttgaaatac ataagcggat tatagtaggt atttgccaat acccggaagg
     2761 caaaagaaac agcaagaaca ggaatgaaag ggaatattgc ataatagctt tcattgcgca
     2821 cgaaaagttg aacaagcagg ggtagtgcga gaatagatac agcaatcatg atcacgctga
     2881 acatggtaaa aacatgatga aaccgattct cgccggttgt acttttctgc aaaccggtat
     2941 cagtccagat ctgatagata cgcgggttga tagtagccga tacagctgtc tgggtcacat
     3001 ctatcagcaa aactactttc agagcaaagt catagatacc cacatcagat gtagtattgt
     3061 aatagttgat gataaagctg ttgatataga ataccaccca gcccagtatg acatagatat
     3121 aaacagggaa acagaatttg tgcagccctt ccagatagga tctgttgaac cggataccat
     3181 attcacgctg aaaaaaaaca gcagaaagca ggaaaatagc aattccggaa agaaaacgcc
     3241 cccaaatggg ccctgttagt gcttcgggat agcgatacac acctatggta catacagcaa
     3301 tggtcaccac aaaattgaac aggttgaaca ccacatgtct gacaggttta tcacggtaat
     3361 acagaaagtt gatataggtt ctgaagaaag cattgcatac gccggtaatg acacacatga
     3421 atccaaatgg gaaaaaatcc agctcaccgt ggaataccat ttgaaaaatg aaggagccaa
     3481 gcaacagaaa tactgttatc agtacaatgc cgataaccag caatgcgcca acaacagacc
     3541 ccacgaatgc cttcatctgc tctggatcct tcttataccg gaaatgctgg ataccgacat
     3601 agttatccat gccataattg gcggtaaact ggataaaaat ggtaaaggcg atataaagtg
     3661 ccagctcacc atacaatgca gtggaaagat tttctgtata gggaaacagc aacaccacac
     3721 tggtcaggag cggcatcata ttgcccatgg taaacagggc tgaagctttt acaatacctt
     3781 ttatcatgca ggccggttcc tgcaaatgta tcatttatgc ccgcaggccc tgggttgctg
     3841 ccaaaaaaaa atccgggagg cataacctcc cggacactga ctattggcaa tcctggtcag
     3901 tctttctttt tcagcaggat gagatcactg atctgtactt ctgatgtata gcgtttcacg
     3961 ccctgcttat cagtatatga tttatggatc agtttgccct ctacagccac ttcagagcct
     4021 tttacaagca gtttctctgc caggccggcc agcttgcccc aggccactac ctgatgccat
     4081 tgggtgtcgg ttacccgctc gccattggca tttttgtagg actcattggt agccagattg
     4141 atcttggcaa gttttctgtc atttcccatt tcaatgatct cagggtctgc acccaggtgg
     4201 ccgatcagct gaactttgtt tctcattgta ctcataacat ctttgattta agcgttttac
     4261 atttttaata catcactgaa ctgtctgttt cgaaacctga cggtgcaaag atttcccggg
     4321 tttttccaat tattcgtata ataaacgttt atatccgata ataaacgttt gcaactggtt
     4381 attgtcggct ttacaggctt gtggtaaggc cttcaacaaa agcagcattt tgcagtattt
     4441 ttgccggtct atgttctgga gttcacttcc gccacaaaaa gctgtattta ttgttggata
     4501 ctggaattcc ggcactactt tactgaccga tatcttacgc aaacatcctg atctgcatct
     4561 ggccagaggt cgtcgtttgc ccaatctgga agaaagaagc atcgaaaaac tttttctaaa
     4621 ggcaggaact ccatttcatc agtttggcga ttacagtgag gttctggccc atggatttga
     4681 aaattatcac tcgccccatt ggaataaaga ccagaccgct gattttttga gagcttttca
     4741 caggaaattc agaacaccta aaggaaaaga cctgttgctc aaaaacccct ggttgttttt
     4801 ttatcaggaa tggatcaaag aaacttttac aggttatcct gtcagaaaga tcatgatcct
     4861 ccgcaatgga tattcgcagg tggtatcgaa agactattgg ttacgcacgg aaggagagcc
     4921 cgcctcacaa ttgaaggcca gggccatatt ctggcataag gccatggaca gatactttca
     4981 gacctggcac aacgatcctg actgcctcac cattcgatat gaaaacctgt gcagtgatcc
     5041 gatggccatc gtcaggcagg tatgcctgca cctgaacatt gatcctaatc cacttacacc
     5101 tcatcttccg gatgcttttg aaaacaggct caccagatgg catcagctgg aagacggact
     5161 caaagagatg gtgcatgctg agatcagtgc tgttcaggat aggatggaca aaatctttcc
     5221 tctccggaat ccgcttacct gaatctgttc ggaattaaaa ataacatact atatttacgc
     5281 atgttcaatt catgcaaaac catagcttat gaccctcaag cgatgtcagg aatgcgaacg
     5341 ggagatcctg ggacgcacag acaaaaagta ttgttcagac atgtgtcgca acaattacaa
     5401 caacagagaa agaagtaagg agcatcagat cgtaagacaa atcaattgta tgcttcttcg
     5461 caaccgtaag atattgtccc ggttgttgac aagcaaagtc agcaagatat cgcgtaacaa
     5521 actgatggaa cagggctttc atttcggcta ctgcacatcg acttatacca cccgaaaagg
     5581 agagacctac ctgttttgtt atgagtatgg ttttctggag ctggctgatg gacaattctg
     5641 cctggtcaaa cgtgcagagc agtattaatg atctgacatt gcaattcgtc ctgacgattg
     5701 ataagtgact gtttcctgct tttgcccgtc tatgaccatc gtatcagtag aaagcactcc
     5761 acgatcatca gctaccagtt ctttgtttgc cgtatagtcg gtctgaaacc cggattgtgg
     5821 tgctgtgtgc tggccgaccg aagcctgaga agaagtagcc tgatctttgt ttgcctgtga
     5881 tggctttacg ccgggtgccc gtaaagcaag gggtttacat cctacacgac ttcccggatc
     5941 aaggatacgt atggcggtac tcaggtggtc aggatagtcg cctatacccc atcctgtgtt
     6001 ggtggtttcg atgtaatagt atttttcacc ctggtactcg accccagttc cgtctagatc
     6061 gccatcgact gcaagcccca cacccatatg accttccgga gacagcagga tcgtttcata
     6121 ccccatgctt ctcaacaatg ctgccagcaa gatggcagtg tcttcacaat cgccgccctg
     6181 ttccaccagt gtttctgccg gatatcttgg atattcatat ttgccgtcat cccgataccg
     6241 aaaatgttgc acgaaactgg caacaaactc gacagtttct ttctgattca gcttgtactg
     6301 cctggccatt cggtttagct ccagagcaaa atcatctgcc acattccttc cgggtgtctc
     6361 cttcatgtac acggtaaaat cgttgtaatc acgtttgatc tgcttgtaat atttgtaatc
     6421 atctgcatca aaggagtatc ggaggttata ccgtttacct tcgaactccc agaaatagct
     6481 tttttccaga gtagttgcgt gcagagttgt ggacagcaac atcgttgtgc caatcaacag
     6541 ggtgcagatg tatgtattca taaggtgcat ttgaatgtat taaccgatca gagtagcatt
     6601 tttatttatc attcaaaaac tttaccaatt ttttaagcat aacgcaatca atcctgaaaa
     6661 tattttccaa attgaactaa cccattctaa atttgtgcgc cgtgggtgct acgtatatta
     6721 ttgaaaccat tcgctgcagg cttcgtcctc tggagcctga tgacgctgaa aagatgtatg
     6781 cattaaacct ggatccggag gtgatcaggt ttaccggaga tgcctctttc agcagtgtag
     6841 atgaagcccg gaccttccta aaagcatatg atcagtataa gaagtatggc ataggcagat
     6901 gggtggtaga gcggaaggaa gacaaagagg tgataggttg gtgtggactg aaattccatc
     6961 cggacgatga tctgtatgat attggataca ggtttttcag gaagtactgg ggccagggct
     7021 acgcaactga aacagccgga gcctgtctgc gatacggcag ggagcagctt gggctagctg
     7081 aagtgtacgc ccatgccatg accggcaata ttgcgtcgtg gaaagtgctt gaaaaacttg
     7141 gaatgaagcg aatggaggag atcatgatgg acggtgagcc ggcttatctg tataaggtac
     7201 aattctaacc ggcccagttt tctctgtcca gacttctgaa ttgtatggct tctgcgaggt
     7261 gttcaatacg tatatcagaa gcgtctgcaa gatctgctat cgttctggct actttcagga
     7321 tcctgtcata ggctcttgct gacaagccca gtctttccat agctttattg agcagcattt
     7381 gacctggttg atctatccgg caccattcac gcacagcaga agagggaagc aaagcattgg
     7441 atccataact acctgatgca tatctgtgtt cctggatctc tcttgccctt atgaccctgt
     7501 ctctgatgga tgcacttttc tcttcttctt taactgcggt cagttcttca atggcaacag
     7561 gtgttacttc cacatgcagg tcaatgcgat ccagtaatgg tcctgagatt ttattgagat
     7621 atcgttgtac gacacccgga ccgcaaacac aatctttttc cggatggttg tagtagccgc
     7681 aaggacaagg gttcatactg gcgatcagca tgaagttggc gggatagtca acagaaaagc
     7741 gggctcttga aatggtcact ttcctttctt ccataggttg ccggagcact tcaagggcat
     7801 ttcgacgaaa ttcaggcagt tcatccagaa aaagaactcc attgtgtgcc agggagatct
     7861 ctcccggttg gggattattt ccaccaccca caagagccac atcgctgaca gtatggtgcg
     7921 gcgaacggaa ggggcgatta tacatcagtg aagcaccgtg ccccagcttt cctgccacag
     7981 aatggatctt ggtagtttca agagcttcat ggagtgtcag tggtggcagg atggtaggca
     8041 ttcttttagc cagcatggtt tttccagcgc ccggtgggcc gatgaggatc acgttatgtc
     8101 cgccggctgc tgcaatttcc agagcccgtt tgatattttc ctgacctttc acatcgctga
     8161 aatctacatc atgctgaccc aggtggtgca tgaattcatc gcgggtgttg atatgaaccg
     8221 gttcaatctt cttgatatcc atcaagtggt caaccacttg attcaggtgg gtaacgccca
     8281 gcacatcaag attatcaact attgccgctt ctctggcatt ttgttccggc aggataaagc
     8341 ttttaaaacc ttcttttctt gcctggatag caatgggcaa tgctcctttt acgggttgaa
     8401 tctgtccatc cagagagagt tcgcccatga tgaggtaatc tttgagatgt gtatcaggga
     8461 tatttccggt gcaggccagc aggccgatgg ccataggcag gtcataaccg ctgccttctt
     8521 tccggatatc agccggtgcg agattgaaga catatttttt tcttggtatt cgaaagccgt
     8581 tatggcttat agcagtttcg attcgctgcc aggcttcttt cactgcatta tcgggaaggc
     8641 ctacgatgaa gcttttcatc tcagtaccgc cggaggtcat attgacctca atagtaatgg
     8701 tatgtgcctg cacgccgtgc acggcgctgg cgaaggtttt aactagcatg cgggtcaagc
     8761 atttgggcaa acttactcta tagagttgat aaaagcagat atgaaagagt ggaaaaacaa
     8821 aaagccccgt caaatggacg gggctttttc aaaaatcggc gacgacctac tctcccggat
     8881 tacctccagt accatcagcg ctgtggggct taacttctct gttcggaatg ggaagaggtg
     8941 gacacccacg ctatagtcac catagactca tgcggcatac gccaataagt ttgacatgcg
     9001 tttggagttg tatgaaactc tgttcagtaa aaaaaatgaa agcttacggg taattagtac
     9061 tgctcggctt tgatgttacc acctttacac ctgcagccta tcaacgtcgt catcttcaac
     9121 gacccttaaa agagatctca tcttgaggcg ggtttcgtgc ttagatgctt tcagcgctta
     9181 tcccttccga acatagctac cctgcgatgc agctggcgcc acaacaggta cactagaggt
     9241 tcgtccaata cggtcctctc gtactagtat cagctcctct caaatctcta acgcccataa
     9301 cagataggga ccgaactgtc ttgcgacgtt ctgaacccag ctcgcgtgcc gctttaatgg
     9361 gcgaacagcc caacccttgg gaccttctcc agccccagga tgcgacgagc cgacatcgag
     9421 gtgccaaacc tccccgtcga tgtgagctct tgggggagat cagcctgtta tccccggcgt
     9481 accttttatc ctttgagcga tggcccttcc atgcggaacc accggatcac tatatcccac
     9541 tttcgtgcct gatcgacttg ttggtctctc agtcaagcac ccttatgcta ttgcactcga
     9601 agcacggtta ccaagcgtgc tgagggtacc tttgaaagcc tccgttactc ttttggaggc
     9661 gaccacccca gtcaaactac ccaccatgca ctgtctccct cattgagggg ttagaatcca
     9721 atcaaataaa gggtggtatt tcaaggttga ctccacgacc actagcgtga ccgcttcaaa
     9781 gtctcccacc tatcctacac atcatttaac cgaattcaat gcaaagctgt agtgaaggtg
     9841 cacggggtct ttccgtcccg ttacgggtaa tcggcatctt caccgatgct acaatttcac
     9901 cgagctcgtg gccgagacag tgctc
//
LOCUS       DAOMAX010000079         9908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_928_length_9908_cov_5.377753, whole genome shotgun sequence.
ACCESSION   DAOMAX010000079 DAOMAX010000000
VERSION     DAOMAX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9908
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_928_length_9908_cov_5.377753"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PK742_05825"
     CDS             <1..1305
                     /locus_tag="PK742_05825"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQU76142.1"
                     /translation="LKPGAQVMFVRNDVGEHRRYYNGKLATVQRINGNEITVAMKDSG
                     TELLLEKEEWKNIRYKLNKENDRMEEEELGSFKQYPVRLAWAITIHKSQGLTFDKVMI
                     DAGQSFAAGQVYVALSRCTTLDGLVLLSRIGQNSILTEPRITEFSSRQTAESSLQQTL
                     EKEKKIFQAGRLLQAFDLQKLIHRLKDFPELVLSKDLPDKGAAYELSQQLIAAAIETD
                     GIVGKFRDQLRDMLAKDTDAAQIRERLDKAAHWLASQIGKKMLQPLADHLDDLKKAKR
                     VRQYTKSVQEVYASVQQNIMHLYGLEYDGSVIQKGALDTYLPVPAEQVLVKKPKEKRA
                     PKGETVRITLELFKQGKSAEVIAMERNLSPNTIESHLAQLVGLGEVLITQIVSDDKID
                     QIKAACLREPNASLTDLKKKLSDDISFGEIKAVQAHMRLSEE"
     gene            1423..1824
                     /locus_tag="PK742_05830"
                     /pseudo
     CDS             1423..1824
                     /locus_tag="PK742_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            complement(1905..2423)
                     /locus_tag="PK742_05835"
     CDS             complement(1905..2423)
                     /locus_tag="PK742_05835"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HQU76143.1"
                     /translation="MAIAAPFNLKQWINDNRDKLKPPVGNQQVYRLNEDFIVMVVGGP
                     NARKDYHWEEGEELFYQVEGDVTVRIQEDGKPRDIVIKEGDMFLLPPRVPHSPRRPAN
                     TVGLVIERYRKPDEKDGFHWYCENCNNLLYEHYFELTDIVTQLPKVMQTFYDSEDLRT
                     CKNCGTVMSKPQ"
     gene            2480..3271
                     /locus_tag="PK742_05840"
     CDS             2480..3271
                     /locus_tag="PK742_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU76144.1"
                     /translation="MDLSLKGKHALVCGSTQGIGKAIAEELAVMGATVILMARNAEKL
                     LQVAAALPSPHGQEHRYITADFSLPETVKVAVEEFLSVFGRPVHILINNTGGPPSGPI
                     TEAPIASFEAAFHAHLVNNHILATTLLPGMRESGYGRIINIISTSVKAPLQNLGVSNT
                     IRGAVANWAKTWANEVAPYGITVNNILPGATETERLSTIVKNKAGKSGTSEEEAAASM
                     LKEIPMKRFGRPEEVAQAAAFLASPSSGYITGINLPVDGGRTPNL"
     gene            complement(3272..3778)
                     /locus_tag="PK742_05845"
     CDS             complement(3272..3778)
                     /locus_tag="PK742_05845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQU76145.1"
                     /translation="MIIEKNRVVSLAYRLRRDNAEGEMIETVDSSQPFVFLFGAGGLL
                     PEFESNLSGKDVGDSVDFGIEAANAYGLPDDNALEFVPKDIFMIDGQLAEDLLTIDGF
                     VNLRDQEGRLMRARVAEVNEAEVLLDFNHPLAGQDLFFSVDVLEVREATEEEIEHGHV
                     HGPGGHHH"
     gene            3909..5858
                     /locus_tag="PK742_05850"
     CDS             3909..5858
                     /locus_tag="PK742_05850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQU76146.1"
                     /translation="MKKYLVPVLIWLGTALPAMAQNGTEDPVLFTVAGEPVTRSEFLY
                     VYQKNNPTKQGDFSEASLQEYLDLYINFKLKVAEAHALRIDTTRKVREELDKYGDQLI
                     KSNFDKEVLEAAIVKHYDRMGTERLVYHVMQGLGKESTPADSAAALAKINEAWEKVQK
                     GADFENTAREYSSDPNISASGGRVGWVTGFSIPDMRFEDMAFETKVGGVSPVFLTKYG
                     YHFLLVKEERPASGQVHVQHILIKTNPKDDNAANTRQEALADSIYFMLMNGADFNELC
                     DRYSDDQGTAKRGGQLDWFGVGKMVQPFEEAAFSLKNPGDISKPVKTAYGWHIIRLQE
                     KRGIAPFEDARVDIKSRIERTSQYSELRNDYVSKVKQTYKFMEYPDNIAAVVATLDST
                     FLNNEWSADKAKGMDKPVFTIGSKTYTQDDLAITLQVKQRTSRDRDIQGKFRSIYEAS
                     RDNMLIEYDMSARNEDFRRLMQEYRDGIPLFALLEQKVWTAAAQDTAGLEAFYDMHKS
                     DYMWEERVDASIYKCADAATAKAVRKMVKKNTADSLILQKMNTDSVVVVNIEHNKFLN
                     GQNSNVDAMNKKPGMSEDILGSNGNITFIKLHSVIPPMPKTLKEARGYVISDYQDQLE
                     QEWITSLREKYPVVVNEKVFNSMVQ"
     gene            5860..6714
                     /locus_tag="PK742_05855"
     CDS             5860..6714
                     /locus_tag="PK742_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76147.1"
                     /translation="MKNLHASAITGWLLLLTAFGCGNRNQSPAGEPLARVYDQYLYAE
                     DIKGLTGEDTSPEDSAAIVREYIDNWVRHQLVLRVAESNLPQQLEDIEKQAEDYKESL
                     LIYAYERRWLAENLDTLIAEDTLKSYFDRNRKDFTLKSDIYRLSYAVMPVAVSGYDTL
                     RNWFVKDISKYRNELESFSLTHCTNYVYESQTWLDEASLFRLLPLSLYENGRLRSNQV
                     VEMADENNRYLVKVHEYVVGGNPAPFDYVREDIRRILINKQKLDVLKSNYARMYEDAM
                     QRNNAEIY"
     gene            6716..8068
                     /locus_tag="PK742_05860"
     CDS             6716..8068
                     /locus_tag="PK742_05860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQU76148.1"
                     /translation="MNKLILLTLSLLAGTALSAQKTIDRIVGQVGDKIILQSEVESIY
                     YQEASNGEVADDLRCYILQELVTQKLLLIQAEKDSVVVTDEQVEYELERRLRYYESLF
                     GSRDKMEEFYGKTFQEMKEEFREDIRNILLSDQMKSNIAGDMTVSPSEVKDFFNNIPK
                     DSLPYFDAEVEVAQLTIKPKPTAEQKAYAKQKAEEIHQRLVDGDSFDLLASLYSEDPG
                     SKEDGGSLGCQTRGTFVPEFEAAAFKLQPDEISNVVATQFGYHIIKMDSRQGDKVCLR
                     HILITPPVTNTNVTIATKKLDSIRTQIMAGNISFRDAAAKFSDDEYSKLVGGDMTNPQ
                     TGSSLFAIDEMDPELYYAIEKLKPGEVSAIVQFTDYDGSRGLRIVQLKSQSAPHVANL
                     EDDYYRLQAAALNNKQQEALNEWVDQKIKSVYLRVDPIYDGCENMQELIRASYDLGRK
                     "
     gene            8075..9040
                     /locus_tag="PK742_05865"
     CDS             8075..9040
                     /locus_tag="PK742_05865"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQU76149.1"
                     /translation="MKQFNSDVEAIDAMAADYKKLGDEIHKVIVGQEEVVKLVLMSIL
                     SNGHSLLVGVPGLAKTLLIKTIADVLELSFKRIQFTPDLMPSDITGTEILDEHRNFKF
                     LKGPLFAHIILADEINRTPPKTQAALLEAMQERNVTVGGEVHKLPLPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNISLDYPSFEEEVNVVKTTTIDSKPELHKIIGDEEIRFYQH
                     LIRRIPIADNVLEYAVQLAGKTRPGTSGAHAVANEFLAWGAGPRASQYLVLGAKCHAA
                     INGKYSPDMEDVRAVAFPVLRHRVLKNYKAEAEGISVEEIIRKLM"
     gene            complement(9295..>9908)
                     /locus_tag="PK742_05870"
     CDS             complement(9295..>9908)
                     /locus_tag="PK742_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQU76150.1"
                     /translation="DLEALRAKKAECEKEIAEREAKIAAGGMKKGEVNFNQNKIDTLK
                     NEVKDLDEQLTNIPQWLESARNFEWGGDLGGSDETVLTRLFDCPVMVYNWPAEVKAFY
                     MKRDPNDEKYVKGVDVLAPEGYGEIVGGAEREDNIDFLIERINNEGLPMEAFQWYLDL
                     RRFGTVPHAGFGLGLERFVMWFTGIQHIRESIPFPRFYGRLKP"
BASE COUNT         2700 a         2532 c         2525 g         2151 t
ORIGIN      
        1 ctcaaacccg gcgcacaggt catgttcgtg cgcaatgatg tgggtgaaca ccgccgttac
       61 tacaacggca agctggccac tgtgcagcgg atcaacggaa acgagatcac cgtggccatg
      121 aaagacagcg gcaccgaact gctgctggaa aaagaagagt ggaagaacat ccgctacaag
      181 ctgaacaagg agaacgaccg catggaagaa gaggaactcg gttccttcaa acagtatccg
      241 gtcaggctgg cctgggccat caccatccac aaaagtcagg gcctcacgtt cgacaaggtc
      301 atgatagatg ccggacagag ttttgcagcc ggacaggtct atgtggcgct cagccgatgc
      361 accaccctgg acggactggt actgctgtca cgtatcgggc agaacagcat cctgactgaa
      421 ccccgcatca cagagttctc ttcccggcag acagcagaaa gttcactaca gcaaaccctt
      481 gaaaaggaga aaaagatctt ccaggcgggc agactgttgc aggcattcga tctgcagaaa
      541 ctcattcatc ggctgaaaga ttttccggag ctggtgttga gcaaagacct gcccgacaag
      601 ggcgctgctt acgaactgtc gcaacagctg atcgctgctg ccatagagac ggatggtatc
      661 gtggggaagt tccgcgatca gctgcgtgac atgctggcga aggatacgga tgcggcacag
      721 atccgcgagc ggctggataa agctgcacac tggctggcat cacagatcgg caagaaaatg
      781 ctgcaaccac tggccgatca tctcgacgac ctgaagaagg cgaaacgggt aagacaatac
      841 accaaatctg tacaagaggt ctatgcatcc gtgcagcaaa acatcatgca tctgtatggc
      901 ctggagtacg atggttcggt catacagaaa ggagcattgg atacatatct gcctgttcct
      961 gctgagcagg tgctggtcaa aaaaccaaaa gaaaaaagag cacccaaagg cgaaacggta
     1021 cgcatcaccc tcgaattatt caaacaggga aagagtgcgg aggtcatcgc tatggaaagg
     1081 aacctgtcgc ccaacaccat tgaatcgcac ctggcacaac tggtaggact gggggaagtg
     1141 ctcatcaccc agatcgtatc agatgacaag atcgatcaga tcaaagcagc ctgtttacga
     1201 gagccgaatg catcgctcac cgatctgaaa aagaagctga gtgatgatat ctcgttcggc
     1261 gagatcaagg cggtgcaggc acatatgcgt ctgtcagagg aatagcgaag gcatcaatgt
     1321 aagaagggaa cacggattta actgatgaga cagattaagc agatgatcag accttgcagg
     1381 ttaatcttaa tgagtattta ttagaatata tttataccat ctatgaaagc ggtcaaatat
     1441 ttggatcaag atatcacaga gctggtaatt cactgttttt ataaagttta taatacgctg
     1501 ggctatggct ttcttgagcg tgtgtacctg aatgctttaa tgatagagct caagacagtt
     1561 ggtttgcgga cttagaagga gcgtatggtc aaggtattct atgataataa agaagttgga
     1621 acgtacctac cagatctgat cgttgaagat caggttattc tggaactgaa agccgcagag
     1681 acactaatgg aagaacatga ataccaactt ctgaactatc tgaaggctac agatctggag
     1741 gtaggactat tgctgaattt tggaaagaag gcacagatca gacgcaaaat attcgaaaac
     1801 aaatttaaga caggtggagc ttgagaatgt ccgacgaaat aaaatcagtt ccaatcagtc
     1861 caatccctgc ctgacgcagt caggcgtcca atctgtgttc cgccttattg cggttttgac
     1921 atgaccgtac cacagttctt gcaggtgcgc aggtcttcac tgtcataaaa ggtctgcatt
     1981 actttgggca gctgggtcac gatatcggtc agctcgaaat agtgttcgta cagcaggttg
     2041 ttgcagttct cacagtacca gtggaagccg tctttttcat ccggtttgcg gtagcgttcg
     2101 atcaccagac ctacggtgtt ggcgggacgt cgtggtgaat gcggaacccg tggaggcagc
     2161 aggaacatat cgccctcttt gatgacgatg tctctgggtt tgccgtcctc ctgaatacga
     2221 acggtcacat caccttccac ctggtagaac agttcttcac cttcttccca gtggtagtct
     2281 ttcctggcgt tcggcccgcc cactaccatc acaataaagt cttcattcag ccgatagacc
     2341 tgctgattac ccacgggtgg tttcagcttg tcgcggttgt cattgatcca ttgcttcagg
     2401 ttgaaaggtg ctgctattgc catatctttg gatgtttacg gggtataaat gtaacccatt
     2461 cagtacagaa agcgtaaaga tggatctctc attgaaaggt aagcacgcac tggtttgcgg
     2521 cagcactcag ggcatcggaa aagccatcgc agaagagctg gcggtcatgg gtgccacggt
     2581 cattctgatg gcacgcaatg cagagaaact gctacaggtg gcagcagctt taccatcgcc
     2641 gcacggacag gaacaccggt acatcacagc cgatttttca ctacccgaaa cggtcaaagt
     2701 tgcggtagaa gaatttctct ctgtcttcgg ccgccctgtg cacatcctca tcaacaacac
     2761 cggtggacca ccttccggac cgatcacaga agcgccgata gcatcctttg aagcagcatt
     2821 ccacgcccac ctggtgaaca atcatatcct ggccaccacc ttgttgccgg gcatgcgtga
     2881 aagcggctat ggtcgcatca tcaatatcat ctccaccagt gtaaaagctc cgttgcagaa
     2941 cctgggagtg agcaatacca ttcgcggagc agtagccaac tgggccaaga cctgggccaa
     3001 tgaggtagca ccatatggta tcacggtcaa caatatactg cccggcgcta ccgaaacgga
     3061 acgcctcagt accattgtaa aaaacaaagc cggcaagagc ggcacctcag aagaagaggc
     3121 cgcggcttcc atgctgaaag agataccgat gaaacgcttc ggcaggcctg aagaggtggc
     3181 acaggccgct gctttcctgg ccagcccttc gtccggctat atcactggca tcaatctgcc
     3241 ggtggacggg ggaaggaccc ccaatctatg atcagtggtg gtgtccgccc ggaccgtgca
     3301 catgaccgtg ctcgatctct tcttcagtag cctcacgaac ttccagtaca tctacgctga
     3361 agaacaggtc ttgtcctgcc agcggatggt tgaaatccag cagcacttcg gcctcgttca
     3421 cttctgccac cctggcacgc atgaggcgtc cttcctggtc gcggaggttc acgaacccgt
     3481 caatggtgag caggtcttcg gccagctggc cgtctatcat gaagatgtct ttaggtacaa
     3541 attccagggc attgtcgtcg ggcaggccat aagcgttggc cgcttcaata ccgaaatcta
     3601 cagagtcgcc cacgtccttt ccagacaggt tagactcaaa ctccggcagc aggccaccgg
     3661 caccaaacag aaagacaaaa ggctgagaag aatcaacggt ttcgatcatt tcgccttccg
     3721 cattatcacg acgcaagcgg taggccaggg agaccaccct gttcttttcg atgatcataa
     3781 tgaaatgtta agagccaaag gtaggtaaat aatcagcaag gacgggcgtt tttggggtcg
     3841 gtatgctgcc atccaacggc caaaaaatgg taattttgcc tcctcttaaa acgaatcata
     3901 ccgccatcat gaaaaagtac ctagtacctg tgttgatctg gctgggcaca gccctcccgg
     3961 ctatggccca gaatggcacg gaagatccgg ttctgttcac agtagccgga gagcccgtca
     4021 cccgcagtga attcctctat gtgtatcaga agaacaaccc caccaaacag ggtgattttt
     4081 ctgaagccag tctgcaggaa tacctggatc tctacatcaa tttcaaactg aaggttgctg
     4141 aagcgcacgc cctgcgcatt gacaccaccc gcaaggtgcg ggaagaactg gacaaatacg
     4201 gcgaccagct catcaagtcc aacttcgaca aggaagtact ggaagctgcc atcgttaagc
     4261 attatgaccg catgggcaca gaacgtctgg tgtatcatgt gatgcaggga ttgggaaagg
     4321 aaagtacacc ggcagattct gctgctgccc tggcgaagat caacgaagcc tgggaaaaag
     4381 tgcaaaaggg tgctgacttt gaaaataccg cccgcgaata ctccagcgat ccgaacatca
     4441 gtgccagtgg tggcagggta ggctgggtga ccggcttcag cattccggac atgcgttttg
     4501 aagacatggc tttcgagacg aaggttggtg gcgtgtctcc cgtgttcctg accaaatatg
     4561 gctatcactt cctgctggta aaagaagagc gcccggccag tggtcaggtg catgtgcagc
     4621 acatcctcat caagaccaac ccaaaagacg acaatgccgc aaacacacgt caggaagctc
     4681 tggcagacag catctatttc atgctgatga acggggcaga tttcaatgaa ctgtgtgacc
     4741 gctattctga tgaccagggc acagccaaac ggggcggaca gctcgactgg tttggcgtag
     4801 gcaagatggt acagcctttt gaagaagctg ccttctccct gaagaacccc ggtgatattt
     4861 ccaaacctgt gaaaaccgct tacggctggc atatcatccg cctccaggaa aagcggggta
     4921 ttgctccgtt cgaagatgcc cgcgtggaca tcaagagccg tatagaacgc accagccaat
     4981 attctgaact gcgcaatgac tatgtgagca aggtgaaaca aacctataag ttcatggagt
     5041 atccggacaa cattgcagct gttgtggcta cccttgacag caccttcctc aataatgagt
     5101 ggagtgcaga taaggctaaa ggcatggata aaccggtatt caccatcggc agcaagacct
     5161 atacccagga cgatctcgcc atcaccctgc aggtgaaaca acgcaccagc agagaccgtg
     5221 atatccaggg taaattccgc agcatctacg aagcttccag agacaacatg ctgatagaat
     5281 acgacatgtc agcccgcaac gaagacttcc gccgcctgat gcaggagtac cgcgacggta
     5341 ttccgctgtt cgccctgctg gaacagaaag tatggaccgc agctgcgcag gataccgctg
     5401 gtctggaagc tttctatgac atgcacaaga gcgactacat gtgggaagaa agggtggacg
     5461 ccagcattta taagtgcgct gacgctgcta ctgccaaggc tgtcagaaaa atggtgaaga
     5521 agaatactgc cgatagtctc attttgcaga agatgaatac tgacagcgta gttgtggtca
     5581 acattgaaca caacaaattc ctgaacggtc agaacagcaa tgtggatgcg atgaacaaga
     5641 agcccggaat gagcgaagat attctgggct ccaacggcaa catcactttc atcaagctgc
     5701 acagtgtgat accacctatg cccaagacgc tgaaagaagc acgcggctat gtaatttctg
     5761 attatcagga tcagctggaa caggagtgga tcacttctct ccgcgaaaag tatccggtgg
     5821 tagtcaacga gaaggtattc aattcaatgg ttcaataatt tgaagaacct ccatgcatcg
     5881 gcaattacag gctggctcct gctcctcacg gctttcggct gcgggaacag gaaccagtcg
     5941 cccgccggtg aacctctggc acgggtatat gatcaatacc tctatgcaga ggatatcaag
     6001 ggattgaccg gcgaagacac ttcaccggaa gacagtgccg ccatcgttcg tgaatatata
     6061 gacaactggg tacggcacca gctggtattg cgggtggccg aaagcaacct tccgcagcag
     6121 ctcgaagaca tagagaaaca ggcggaagac tataaagaat ctctgctcat ttatgcatat
     6181 gaacggcgat ggctggctga gaacctggat accctgatcg ccgaagatac cctgaagtcg
     6241 tactttgaca ggaaccggaa ggattttacg ctgaagagcg acatctaccg gctttcctat
     6301 gcggtaatgc cggtggccgt gagcggttat gatacccttc gcaactggtt tgtcaaagac
     6361 atcagcaagt accgcaatga actggaatct ttcagcctga cccattgcac taattacgta
     6421 tatgaaagtc agacctggct ggatgaagca agtctattca ggttactgcc actcagcttg
     6481 tatgagaatg gcaggctgcg ctcaaaccag gtggtagaga tggcagacga aaacaaccga
     6541 tacctcgtta aagtacacga atatgtggtg ggcggaaacc ctgctccttt cgattatgtg
     6601 cgggaagaca tcaggcgcat actcattaat aaacagaaac tggacgtgct gaaaagcaat
     6661 tacgcacgca tgtatgaaga cgccatgcag cgcaacaacg ccgagatcta ctgatatgaa
     6721 caaactcatc cttcttaccc ttagcctttt ggcgggcact gccctgtctg cacagaagac
     6781 catagaccgc attgttggcc aggtgggcga caagatcatc ctccagagtg aggtggaaag
     6841 catctattac caggaggctt ccaatggtga agtggcagac gatctgcgct gttacatcct
     6901 ccaggaactg gtgacccaga aactgctgct catccaggct gaaaaagaca gtgtagtggt
     6961 gaccgacgaa caggtggagt acgaactgga aaggcgtttg cgctactatg aatcgctgtt
     7021 cggttccaga gacaagatgg aagaattcta cggcaagacc tttcaggaaa tgaaagaaga
     7081 attccgtgaa gacatacgca acatactcct gtctgatcaa atgaagagca acattgccgg
     7141 cgatatgacc gtttcgccca gcgaagtgaa agatttcttc aacaacattc cgaaagacag
     7201 cctgccctac ttcgatgcag aagtggaagt ggcacaactg accattaaac cgaaaccaac
     7261 cgcagaacaa aaagcatatg ccaaacagaa agctgaagag atccaccagc gcctggtgga
     7321 cggcgatagt tttgatctgc tggccagtct gtactcagaa gaccccggtt ccaaggagga
     7381 tggtggttca ttaggctgtc agacccgtgg tactttcgta cccgaattcg aagctgcagc
     7441 tttcaaactg cagccggatg agatcagcaa tgtggtagcc acccagttcg gataccacat
     7501 catcaagatg gacagccgcc agggcgataa ggtctgtctc agacatatcc tcatcacccc
     7561 gccggtcacc aataccaacg tgaccattgc caccaaaaaa ctggatagca tacgcaccca
     7621 gatcatggca ggcaatatca gtttccgtga tgcagcagcg aaattcagcg acgacgaata
     7681 ttccaaactc gtgggtggcg atatgaccaa tccgcaaacc ggttcttccc tttttgccat
     7741 cgacgagatg gacccggaac tgtattacgc cattgagaaa ctcaagccgg gtgaagtgtc
     7801 tgccatcgta cagttcaccg attacgatgg ctcccgcgga ctgcgcatcg tacagctcaa
     7861 gagccagagt gcgccacacg tagccaacct ggaagacgac tactacagac tgcaggcagc
     7921 agcgctcaac aacaaacagc aggaagcgtt gaacgagtgg gtggaccaga agatcaaatc
     7981 tgtttacctt cgggtggacc ctatatacga cggttgtgag aatatgcagg aacttatccg
     8041 ggcatcctat gacctcggca ggaaataaga aaacatgaag cagttcaaca gcgatgtgga
     8101 agccattgat gcgatggcag cagattataa gaaactgggc gacgagatcc acaaggtcat
     8161 tgtaggtcag gaagaagtgg tgaagctggt actgatgtcc atcctcagca atggacacag
     8221 tctgctggtg ggtgtgcccg gtctggcgaa gaccctgctc atcaaaacca tcgccgatgt
     8281 actcgaactc agcttcaaac gcatacagtt cacgcctgac ctcatgccga gtgacatcac
     8341 cggtacggag atcctggacg aacaccgcaa cttcaaattc ctgaaaggtc cgctcttcgc
     8401 ccacatcatc ctggccgatg agatcaaccg tacaccgccc aagacccagg ccgctctgct
     8461 ggaagccatg caggaacgca acgtgaccgt gggtggtgaa gtacacaaat tgcctttgcc
     8521 cttcttcgta ctggctaccc agaaccctat tgaacaggaa ggaacctatc cgctgcccga
     8581 agcgcagctg gaccgtttca tgttcaacat cagcctggac taccccagct ttgaagaaga
     8641 ggtcaatgtg gtaaagacca ccaccataga cagcaagccg gagttgcaca agatcatcgg
     8701 cgatgaggag atccgcttct accagcatct catccgccgc atacccatag cagacaacgt
     8761 attggaatat gccgtgcagt tggcaggcaa gacccgtccc ggaacttcag gtgcacacgc
     8821 cgtggccaat gaattcctcg cctggggagc aggaccacgt gccagtcagt atctcgtact
     8881 tggtgccaaa tgccacgccg ccatcaatgg caagtactct cctgatatgg aagatgtgcg
     8941 tgcagttgcc ttccccgtgc tccgacaccg cgtgctcaag aactacaagg cagaagccga
     9001 aggcatcagc gtggaagaga tcatcaggaa gctgatgtga tgaatgagag aattaggtaa
     9061 tttgctaatg tgaaaattag gtaatgtgag aatgagctaa tgtggtaatt agctaatgga
     9121 ttttgaatga taactatcgg ggactaatct ttgaccagcc tattttccaa atagctgatt
     9181 agctaatgag aaaattagtc aatgggtttt gtctcgttgc tatcgcgaat cagtccttta
     9241 tcagcccttt ctccaattga ctaattagct aattctctca ttggctaatt ccagttacgg
     9301 cttcagcctg ccgtaaaatc tcgggaacgg aatggattca cgaatgtgct ggatgcctgt
     9361 gaaccacatg acgaagcgtt cgagtcccag accgaaacct gcgtgcggaa cagtaccgaa
     9421 tctgcgcagg tcgaggtacc actggaatgc ctccatcggc aatccttcgt tgttgatacg
     9481 ctcgatgagg aagtcgatgt tgtcttctct ttcggcacca cccacgatct cgccataacc
     9541 ttccggtgcg agtacgtcca caccctttac atatttctca tcgttcgggt cacgcttcat
     9601 atagaaagcc ttcacttctg ccggccagtt gtacaccatg accgggcaat cgaacagacg
     9661 tgtgagtacg gtctcatcgc ttccacccag gtcgccgccc cactcgaagt tgcgggcgct
     9721 ctccagccac tgcggaatat tggtaagctg ttcgtcgagg tctttcacct cgttcttcag
     9781 cgtatcgatc ttgttctggt tgaaattcac ttcgcccttc ttcatgccac ctgcagcgat
     9841 cttcgcttcc cgctctgcga tctctttttc acactcagcc tttttcgcac gaagtgcttc
     9901 gagatctg
//
LOCUS       DAOMAX010000080         9907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_929_length_9907_cov_4.141799, whole genome shotgun sequence.
ACCESSION   DAOMAX010000080 DAOMAX010000000
VERSION     DAOMAX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_929_length_9907_cov_4.141799"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PK742_05875"
     CDS             <1..1096
                     /locus_tag="PK742_05875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQU76151.1"
                     /translation="GISFYTFQTLSYSIDVYRGTTKAEKHFGYFALFVSYWPQLVAGP
                     IERSGDLLPQLREEFKFEYNRVREGLIRIGWGFFKKVVVADRLSIFVQQVYQHPGEHG
                     GFAVVLATWMFAFQVYCDFSGYSDIAIGSARMMGVNLTDNFKTPYFSKTIREFWQRWH
                     ITLSVWIRDYLYFPMGGSRVTYSRLMFNTMFTFAVMGLWHGANWTFVMFGVIHGVLLS
                     VRRTADKFFPNLKFDLGPRLKWLTFAISAFWVFNLTCIPDIFFCSATSGDAMIQIQSI
                     FTVPNTTHTLIHNSLTGADTSVQFILSWVFIAFLLFFDIRMYTNKDETIEGLITRQPV
                     AVRWTFYVVLLIFVGWFAMTTNAAFIYFQF"
     gene            1098..2168
                     /locus_tag="PK742_05880"
     CDS             1098..2168
                     /locus_tag="PK742_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76152.1"
                     /translation="MKKLFRNIALLLLIYIVISQVAVNSLPFSWGNTRLNTKYEEFKS
                     RVGDFNTIFIGASTTYRHIDPTIFDSRLAEEKPDWHIHSFNFGIPANRTPQSIYALKN
                     LIEMKGNDIEYIVLDLSELTKMGVDNLHKKEMIYWYTRDNIVSVMQTSYDSEKGTLNK
                     IGVPLLHLFSYGEKLLMVGMGSAMLEQHAGINIEELALGPDRNGYYSLDQEMVDDPEG
                     DLAIRYDVLRTQDTIDFRTMQCQKLYDKYRDKGTGYSKTMEQELEDLIKLCNGKGIHM
                     VVMLSQRLGDRYEYLIPLYNRLPEANKVSFANPAEYPELNDRNNLFDLAHLNRQGSIL
                     FTDLFATKFIEKVEAQEGADAR"
     gene            2184..3557
                     /locus_tag="PK742_05885"
     CDS             2184..3557
                     /locus_tag="PK742_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HQU76153.1"
                     /translation="MIRKVRDKIRKAFRDGDFSEIFKKSASGFFISVLGRNFGFVQQL
                     VITNYYGAAAFGVFRVCFSILSLVGIAGRFGVDMAISRFVAQYRKQNRYDLVNEIFQM
                     GLRLVFPIAVILTIGVFFLSPALSEHVYHKPYTGYIQIFAIGILFFVLSGVIEEGIRG
                     LKKIKEYTWINNVSTQAFSTIILLVALFFTTNPYMVNISYVAGLIFTFILGIYYWYKF
                     VPHQKIEEPQLKRKDLLAVSLPMLSAKYLTTLYTWLGTLILAAYVTDDHVGIFNGAAR
                     MSAFATMPLIAVNNISGPRFAEAFGENDEKSLRKTVRLSTRLIFWTAMPIMAAFYLFP
                     KTILGIYGDEFNTSDAILTFAVINAGQVVNFMTGPVTQLLNMTGRQLVTQRYAIITTI
                     TSIGLSFLLIPSMGMLGAAIATAVARTVLNLGCALHIYFTMKIVTIYNPFADLWLLIS
                     KLRRKHA"
     gene            3559..4470
                     /locus_tag="PK742_05890"
     CDS             3559..4470
                     /locus_tag="PK742_05890"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU76154.1"
                     /translation="MNQLHPWPDFLIVGTARSGTTALHEHLGKHPGIFVPLQKEPCYF
                     TFAGEDPSFKDSRHRYTTTKQDYLQLGADCEDRLWGESSTPYLYFFDKSIANIRQQVP
                     GYEAIRIIMVLRDPAERAYSQYMHNRRDLREPLSFEDAIAAEAQRMQENWHFDFFYVD
                     KGFYFQQVQAYLQAFDKVHIILFEEFEKDPVAVVNGVLDFLELPDDVDLGMVKKKNQS
                     GEMKHKWIKRLMSNRTNPVLNGLRRLMSRKARKQLRNYVKGLVLKHNLKKVDMDPATR
                     KQLVELYRRDIEQLQKLIGRDLSAWLR"
     gene            4458..5342
                     /locus_tag="PK742_05895"
     CDS             4458..5342
                     /locus_tag="PK742_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HQU76155.1"
                     /translation="MATLRSIQTFLCIGVQKAGTTTLADILATHPDICIPEVKETKFF
                     YFAEDYSKGIDHYNHTFYRGCSEHKAVGELDPDYIMHPEAAGRVRETLGDGIKLIVVF
                     RDPARRAYSQYLMSRKKGIEKLGFNEALSAEDSRRNWPNYKTVAYVERGLYATQLKRW
                     LELFPRTQFLFLDFEKDIVQQLPETLRHIQEFLGVSVLDLDHTIRSNEASEAVNDSVR
                     DMVRRPNFVKTVLKKLIPSARFRKKARRFMIERNQQAASASRLEEEEAREINRKYFAE
                     EIAGIRELTGLPFEHWSI"
     gene            5339..6673
                     /locus_tag="PK742_05900"
     CDS             5339..6673
                     /locus_tag="PK742_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76156.1"
                     /translation="MSLLTPEKGMFKEPYSVWGKLWLAIMIIVFTTRMPIKTTLVNYY
                     MYFEGFWYMFITAFSFIYLAYVLFQRKGVDEMNYLYVMMFSLPVLSAVAANLAEGQSI
                     PGGLIAQRHWFGLGGIFLIIYLFKANMFTIKDFFRALEWMGWLTLILYLYCYIFLDPR
                     DYLDYSFVGYSDVKGGYRFRFGVDFIAYVALFSTIKFIVDRKRIYILYSIMMLAYLFF
                     LHQGRIVMVSIIGCIFLYYLIEVNWKKTIVYVVTFGSGFLLLMTLLSFLFPEFVGRYV
                     EQYVNVVLVFLGQETGEGSVDARAEEIVIVFAYMINYPIGWIIGVGKYGSDYIDPSEP
                     ITQIAPGDIGIIGSVMTYGIIGTVIMYIQFWIAFRSMRFVKRYKKDVYFMTAKYFLFY
                     CFLTSIAKGNLFSQPGSTAIFILIIYAFKLLEVDMDKRGVPLQPETAVNKAN"
     gene            6678..7664
                     /locus_tag="PK742_05905"
     CDS             6678..7664
                     /locus_tag="PK742_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76157.1"
                     /translation="MPKNKMIMVIGSLNANPADREISREDIRSVQLIHPNREISEVSF
                     FHSWGELGMSSMAVHPDDKMKLYFATSSQVFLFNLHANEQTDLQIPNLTDVHEISMVG
                     DELWISNTKHDEIVAYHIRENRVSRRIDLSDFASSTPEEGEGGEGVEVVDKFHCNLIF
                     QGYDGHLYILVHHTSGRQLIKRIAQKFIKSQGNGGVVNIDTKKRYPLNFKAPHSVRRI
                     NGEYWVFDSGHFELKVFDQQWKLKKSINTKGFGRGGEYQPETGLYYAGVSATRKRYLG
                     LFPGGDSVPNMVQVVKAADYKIEETIMIPNIEQINNVYCIDNDVANKLLQMV"
     gene            7667..8647
                     /locus_tag="PK742_05910"
     CDS             7667..8647
                     /locus_tag="PK742_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HQU76158.1"
                     /translation="MPAESPYPLPTFIVVGANKGGTTSLFHYLQQHPEVYLSPLKEPH
                     YFSKDIDPEQFSKGFAHNKLRDIAAYVSGPMTDHKHAGFVRSWDHYCKLFKFANGAKA
                     IGELSTSYLYSSAAAREIREALGDIRIIICLRNPINRAYSHYRMNLWTGNSKAKTFMQ
                     ALREDQTHEPKVWGNAHLYVEIGLYYEQVKRYYEQFSPDHIKIIFSQELRKHPEKVVR
                     DLYEFIGVNPDFQPDTTGRFNEVYTPKFKGLTWFLNKTGIRPMIKRLSGQRMKQAVVK
                     TMFRNKEDKGSMTDEERNYLADIYREDVDQLSKLIGCDLNHWIPKQEGTS"
     gene            8644..9645
                     /locus_tag="PK742_05915"
     CDS             8644..9645
                     /locus_tag="PK742_05915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU76159.1"
                     /translation="MNRVCVILVNWKTWQDTAECIESLLRADAPGMQIVVVENDSPDD
                     SWEKLNAWARGEVTVEIPADNKLRHLSTPPASKPLQFATGNAGDTQGIQTEAPLVLIR
                     SDKNLGFAGGNNVGLRYMLQHGFDYAWLLNNDTVIEPDAVQALIRHQQSHGDHTGITG
                     SKLRLYHKPEVLQGLGAVFNPKKGTSRIIGAQQEDHGQFDQEAEQITYAIGAAMFVNR
                     KFLEEVGLMSEDYFLYNEENDWSARASRKGWTVGVAADSVVYHKQGASTGNSPKKSKW
                     AIQALRYKYRGKILLYKKYYRKYLPYLYIHLLRRSLRYILRGNLREAGVIYQAILNT"
     gene            9655..>9907
                     /locus_tag="PK742_05920"
     CDS             9655..>9907
                     /locus_tag="PK742_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76160.1"
                     /translation="MIISHKHKYVFVELPQTASSAIARELVANYDGEEILFKHALYRT
                     DFLKKATPEEKQYRVISGLRNPMDICVSNYFKFKTDHENR"
BASE COUNT         2656 a         2275 c         2411 g         2565 t
ORIGIN      
        1 aggtatttca ttttatacat ttcaaacgct cagctattcc attgatgtgt atagaggtac
       61 cacaaaagct gagaagcact ttggctactt tgcgctgttc gtttcatact ggccgcaact
      121 ggtggcagga cctattgaaa gaagcggaga tctgctacca caactcagag aggaattcaa
      181 atttgagtat aacagggtaa gggaaggact tatcaggata ggctggggtt tctttaagaa
      241 ggtggtggta gctgaccgcc tcagcatttt tgtgcagcag gtttatcagc atcccggcga
      301 acacggaggc tttgctgtgg tactggctac ctggatgttt gcctttcagg tgtactgcga
      361 tttttccgga tattcagata tagctatagg ttcagccagg atgatgggag tcaatctgac
      421 agacaacttc aaaactccct acttcagcaa aaccatcaga gagttctggc agcgctggca
      481 tatcactttg tctgtatgga tcagagacta cctgtatttc cctatgggcg gcagcagggt
      541 cacctattcc agactcatgt tcaatactat gttcactttc gcggtgatgg gattatggca
      601 tggagccaac tggacatttg tcatgttcgg tgtcattcat ggtgtcttgc tgagtgttcg
      661 acgaacggca gataaattct tccccaacct gaaatttgat cttggcccca ggttaaagtg
      721 gctcaccttt gccatttcgg ctttctgggt attcaacctg acttgtatac ctgatatttt
      781 cttttgctct gctacctctg gtgatgccat gatccagata cagagcattt tcaccgttcc
      841 gaatactacg cacaccctga tccataacag tttgaccgga gctgatacaa gtgtgcaatt
      901 catcctatcc tgggtgttca ttgcttttct tttgtttttt gatataagga tgtacaccaa
      961 taaagatgaa actattgaag ggttgatcac ccgtcagccg gttgctgtaa gatggacatt
     1021 ttatgttgta ctgctgatat ttgttggttg gtttgccatg accaccaacg cagcattcat
     1081 ctacttccag ttctgatatg aagaagctat tcagaaatat tgctctgctg ttgctgatct
     1141 acattgtgat cagtcaggtg gccgtgaaca gcctgccttt cagttggggt aatacaaggc
     1201 tgaatacaaa gtatgaggaa tttaaaagca gggtaggaga tttcaataca atctttatcg
     1261 gagccagtac tacctatcgt catattgacc ccaccatttt tgattcaaga ctggctgaag
     1321 agaaaccaga ctggcatata cattctttta attttggtat tccggccaac agaacaccac
     1381 aatcgatcta tgccctcaaa aatctgatcg aaatgaaggg caatgatatc gagtatattg
     1441 tactggatct cagtgagctc accaaaatgg gcgttgacaa cctccataag aaagagatga
     1501 tctactggta cacacgagat aacatcgtgt cagttatgca gaccagctat gactctgaaa
     1561 agggcacgct caacaagatt ggtgtgccac tgcttcacct gttctcttat ggtgagaagt
     1621 tgctcatggt tggaatgggc tctgctatgc tggaacaaca tgccggcatc aatatagaag
     1681 agctggcact tggacctgac agaaacggat attattctct ggatcaggaa atggttgatg
     1741 acccggaagg tgatctggcc atacggtatg atgtactcag aacccaggat accatagact
     1801 tccgtaccat gcaatgccag aagttgtatg ataagtacag ggataaaggt accggctaca
     1861 gcaagaccat ggagcaggag ctggaagatc tgataaaact ctgcaacggg aaaggtatcc
     1921 atatggttgt catgttatct cagagacttg gcgacagata tgaatatctg atacctctgt
     1981 acaacagact tcctgaagcc aacaaggttt cttttgccaa tcccgctgaa taccctgaac
     2041 tgaatgaccg aaacaacctt ttcgatctgg cgcatctgaa tcggcaggga tccatacttt
     2101 ttacagatct tttcgcaacg aagttcatcg aaaaggtaga agcacaggag ggtgcagacg
     2161 cccggtgata cgggagcagc gatatgatcc gcaaggttcg agataagatc cggaaggctt
     2221 tccgggatgg cgacttttca gagatcttca agaagagcgc ctcgggtttt tttatcagtg
     2281 tactgggtcg aaatttcggt ttcgtgcagc agctggtcat caccaactac tatggtgcgg
     2341 ccgcattcgg tgtatttcgt gtttgttttt ccatcctgag cctggtaggc atagcgggaa
     2401 gatttggggt ggatatggcc atcagcaggt tcgttgccca ataccggaaa cagaatcgat
     2461 atgacctggt caatgagatc tttcagatgg gactccgcct ggtctttcct atagcagtta
     2521 tcctgaccat aggtgttttc ttcctgtcgc cggctctgtc agaacacgta tatcataaac
     2581 cctataccgg ttatattcag atcttcgcca taggtatcct gttctttgtg ttgagcggag
     2641 ttattgaaga aggtatcaga ggtctgaaaa agataaaaga atatacctgg atcaataatg
     2701 tcagtactca ggctttcagc accatcattt tgcttgttgc cttgttcttc accacgaatc
     2761 cgtacatggt caacatctcg tatgtggccg gactgatatt cacatttatt ctgggcattt
     2821 attattggta caagtttgtt ccccaccaga agatcgaaga gccccagctg aaaagaaaag
     2881 acctgctggc agtgtcactg cccatgttgt ctgccaaata cctgaccacc ctctatacct
     2941 ggttgggcac tttgatactg gcagcctatg tgacagatga tcatgtgggt atcttcaacg
     3001 gtgccgcccg gatgtcggca tttgctacca tgccactaat tgcggtcaac aacatttcag
     3061 gtccgaggtt tgctgaagcc ttcggagaga atgatgaaaa gtcactccgt aagacagttc
     3121 gcctgtcaac ccgattgatc ttctggaccg cgatgcccat tatggcagcc ttctatcttt
     3181 ttcccaaaac cattctcggt atttacggag atgagttcaa tacctcagat gccattctca
     3241 cctttgcagt catcaatgca ggtcaggttg ttaattttat gacaggtccg gttacacagt
     3301 tactgaacat gaccggccgg caactggtca cccagcgtta tgctattatc acaaccatca
     3361 cgagtatcgg ccttagtttt ctgctgatcc cttccatggg aatgttgggt gctgccatag
     3421 ctacagcagt agcacgtact gtgctgaatc tggggtgtgc cttacacatt tatttcacca
     3481 tgaagatcgt gaccatatac aatccttttg ctgacctgtg gctgctcatc tcgaaactca
     3541 ggaggaagca tgcctgatgt gaatcaacta cacccctggc ctgatttcct gatcgttggg
     3601 accgcccgga gcggcacgac cgctcttcat gaacacctgg gcaagcatcc cggcattttt
     3661 gtgcccttgc agaaggaacc ctgttacttc acttttgccg gtgaagatcc ctccttcaaa
     3721 gattcccgtc accgctacac cactaccaaa caagattacc tgcaactggg tgcagactgt
     3781 gaagacagat tatggggtga atcatccaca ccctacttat acttcttcga taaaagcatt
     3841 gccaatatcc ggcagcaggt acctggatat gaagccatcc gcatcatcat ggtgcttcgt
     3901 gatccggcag aacgtgcgta ttcgcaatac atgcacaaca gacgagacct ccgtgaacca
     3961 ctgagctttg aagatgccat tgctgcagaa gcacagagga tgcaggagaa ctggcatttt
     4021 gattttttct atgtggacaa gggcttttac ttccagcagg tgcaggccta tctgcaggca
     4081 tttgacaaag tacatatcat tctgttcgaa gaatttgaaa aggacccagt ggctgtggtt
     4141 aatggtgtgc tggattttct ggaactaccg gatgatgtgg acctcggaat ggtgaaaaag
     4201 aagaatcaga gcggcgagat gaaacacaag tggatcaagc gcctgatgtc taacagaaca
     4261 aaccctgtac tgaacggatt gcgccggttg atgtccagaa aagccagaaa gcagctgcgc
     4321 aattatgtga aaggcttggt gctgaaacac aaccttaaaa aggttgatat ggatccggct
     4381 accagaaaac agctggtgga attatatcgc agggatattg aacaattaca aaaattgatt
     4441 ggcagagatt tgtcggcatg gctacgctga gatccataca aaccttcctg tgtatcgggg
     4501 tgcagaaggc cggcaccacc acactggctg atattctggc cacccatcct gatatctgta
     4561 ttccggaggt caaagagacc aagttcttct attttgctga agattattca aaggggatag
     4621 accactataa ccacaccttt tataggggat gttcagagca taaggcagtg ggagaacttg
     4681 atcccgacta tatcatgcat ccggaagcgg caggcagggt tcgcgaaaca cttggtgatg
     4741 gtatcaaact gatcgttgta ttcagagatc ctgcacgcag ggcctattct caatacctta
     4801 tgagtcgcaa aaaaggaatt gagaagcttg ggttcaacga agcactttcg gcagaagatt
     4861 cccgcaggaa ctggcccaat tacaaaacgg tagcctatgt agaacgcggc ttgtatgcca
     4921 cacaactaaa gcgctggctg gaactgttcc caagaacaca gttcctcttt cttgactttg
     4981 aaaaagatat cgttcagcaa cttcctgaaa cactcaggca catccaggag tttttagggg
     5041 tatctgtgct ggatctggac cacaccatca gaagcaatga ggcgtctgaa gctgtaaatg
     5101 acagcgtgag agatatggtc agaaggccta attttgtgaa aactgtattg aagaaactga
     5161 tcccttccgc cagattcaga aagaaagcac gcaggttcat gatagaacgg aaccaacagg
     5221 cagcatctgc ttcgaggctg gaggaggaag aagcccgtga gatcaaccgc aaatattttg
     5281 cagaagagat cgcaggtatc agagaattga ccggattacc atttgaacac tggagcatat
     5341 gagtttgctg acccccgaaa aaggaatgtt caaggaaccg tattcggtat ggggaaagct
     5401 gtggctggcc atcatgatca tcgtgttcac cacgcggatg cctatcaaga ccacgctggt
     5461 aaactactac atgtattttg aagggttctg gtacatgttc attaccgcat tctccttcat
     5521 ctacctcgca tatgtgctgt ttcagcgaaa gggtgtggat gagatgaatt acctctatgt
     5581 gatgatgttc tcccttccgg ttttgtctgc cgtggcagca aatcttgcgg aaggacaatc
     5641 catacctgga ggattaattg cacagcgaca ttggttcggt ctgggcggta tctttttgat
     5701 catatacctg ttcaaggcca atatgttcac catcaaggat tttttccggg cgctggagtg
     5761 gatgggatgg cttacgttga tattatatct ctactgttat atatttctgg accccagaga
     5821 ttacctcgat tattcctttg tgggatacag tgatgtgaag ggtggctatc gcttccgttt
     5881 cggggttgac tttatcgcct atgtggcgct gttctccacc atcaagttca ttgtggatcg
     5941 caagcggatc tatattctgt actccatcat gatgctggct tacctgttct ttctgcacca
     6001 gggacgtatc gtgatggtat ctatcatcgg ttgtattttt ctctattacc tgatcgaggt
     6061 caactggaag aaaaccattg tgtatgtggt caccttcggt tcgggatttc tgctcctcat
     6121 gaccctgctg tcttttctct ttccggagtt tgttggccgt tatgtagagc agtatgtcaa
     6181 cgtagtgctt gttttcttag gtcaggaaac aggtgaaggc tcggtggatg ccagggctga
     6241 agagatcgtc attgttttcg catatatgat caactatccc atcggctgga tcatcggggt
     6301 aggaaaatac ggttctgact acatagatcc gtctgaaccc atcacccaga tcgctccggg
     6361 tgatattggt atcattggtt ctgtcatgac ctatggtatc ataggcacgg tcatcatgta
     6421 tatccagttc tggatagcct tcagaagtat gcggtttgtg aagaggtata aaaaggacgt
     6481 gtatttcatg accgccaaat actttttatt ttattgtttt ctgacctcca tagcaaaggg
     6541 caatctgttc tcgcagccgg gttctacagc cattttcatt ctcattatct acgccttcaa
     6601 attgctggaa gtagatatgg ataaacgtgg tgtacctttg cagccggaaa ctgcagtcaa
     6661 taaagctaac tgaaactatg cctaagaata agatgatcat ggtgataggc tcactgaatg
     6721 ccaatcctgc tgaccgggag atcagtcggg aggatattcg ttctgtgcag cttatacatc
     6781 ctaacagaga gatatcagag gtgtcttttt ttcatagctg gggagagctt ggtatgagtt
     6841 ctatggccgt tcatccggac gataaaatga aactgtactt tgccaccagc agccaggtat
     6901 tcctgttcaa cctgcatgcc aatgaacaga ccgatctgca gatacccaat ctgaccgatg
     6961 ttcatgagat cagtatggtg ggtgatgaac tctggatctc caataccaag cacgacgaga
     7021 tcgtggctta ccacatcaga gagaacaggg tgagcagacg cattgacctg agtgattttg
     7081 catcgtccac accagaagaa ggagaaggcg gggaaggcgt ggaagtggta gataaattcc
     7141 attgcaacct cattttccag ggctatgacg gacatctgta tatactggtg caccatacct
     7201 ccggccgaca acttatcaag cgcatcgccc agaaattcat caagagccag ggcaatggcg
     7261 gtgtggtgaa catcgatacg aagaagcgct atccgctcaa tttcaaggca ccacacagtg
     7321 ttcgccggat caacggagag tactgggtct ttgacagcgg acattttgaa ctgaaggtct
     7381 tcgatcagca gtggaagctc aagaagagca tcaataccaa aggttttggt cgcggtggtg
     7441 aataccaacc cgaaacgggt ttgtactatg caggagtttc tgctacccgc aaacgctacc
     7501 tggggctctt tcccggtggt gattcggtac ccaatatggt gcaggtggta aaagcagcag
     7561 attacaagat cgaagagacc atcatgattc cgaatattga acagatcaat aacgtttact
     7621 gtatcgataa cgatgtagcc aacaagctgc tgcagatggt gtgaccatgc ccgctgaaag
     7681 cccataccca ttacctactt tcatcgtagt cggagccaat aagggtggca ccacttcctt
     7741 gttccattac ctgcagcaac atccggaggt gtatctgagt ccgctcaagg aacctcacta
     7801 cttttccaaa gacatagacc cggaacagtt cagtaaggga tttgcacaca ataagctgag
     7861 agacattgcc gcctatgtca gcggccccat gaccgatcat aaacacgcag gtttcgtgcg
     7921 tagttgggat cattactgta agctgttcaa gttcgccaat ggtgccaaag ccatcggcga
     7981 actgagtacc tcttacctgt attcttcggc agcagccaga gagatccgcg aagcacttgg
     8041 tgatatccgc atcatcatct gcctgcgcaa tcccatcaac cgggcttatt cacactatcg
     8101 catgaacctg tggaccggca acagcaaggc aaagaccttc atgcaggcgc ttcgcgaaga
     8161 ccagacccat gaacccaagg tgtggggcaa cgcccacctg tatgtggaaa taggcctgta
     8221 ctatgaacag gtgaagcgct attatgagca attctctcct gaccatatca aaatcatctt
     8281 ttctcaggag cttcgtaagc atcctgagaa agtagtccgt gatctgtacg aattcattgg
     8341 tgtgaacccg gattttcagc cggatacgac aggacgcttc aatgaagttt atacgcccaa
     8401 gttcaaggga ctgacatggt tcctgaacaa gaccggcatt cgccccatga tcaaaagact
     8461 cagcgggcaa cgcatgaaac aggctgttgt aaagacgatg ttccgcaaca aggaggacaa
     8521 aggcagtatg accgatgaag aacgcaacta cctggcagat atctatcgcg aagacgtgga
     8581 tcaactatca aaactgatcg gttgcgacct gaaccactgg atacccaaac aggagggcac
     8641 ttcatgaacc gggtctgcgt catactggtc aactggaaga cctggcagga tactgccgag
     8701 tgcatagaga gtttactgcg ggctgatgct cctggtatgc agatcgttgt cgtggagaat
     8761 gattcgcccg atgacagctg ggagaaactg aatgcctggg ccaggggaga agtaacagta
     8821 gaaattcctg ccgataacaa actcagacat ctgagcactc caccagcttc caaaccgctg
     8881 cagttcgcca caggcaatgc gggcgataca cagggcattc aaacagaagc accactcgtg
     8941 ctgatcaggt ctgacaagaa tctgggcttt gccggtggaa ataatgtggg cctgcgctat
     9001 atgctgcagc atggtttcga ctacgcctgg ctgctcaaca atgatacggt catagaaccg
     9061 gatgccgttc aggcactgat cagacatcag caatcacatg gagatcatac cggcatcaca
     9121 ggcagcaagt tacggttgta tcataagccg gaagtgttgc agggtctggg tgctgtgttc
     9181 aatccgaaaa aaggtaccag tcgcatcata ggtgcgcagc aagaggacca cggtcagttc
     9241 gatcaggaag ctgaacagat cacctatgcc attggtgcag ccatgttcgt gaacaggaaa
     9301 ttcctggaag aagtaggcct gatgagtgaa gattattttc tctacaacga agagaatgac
     9361 tggagtgcca gggcttcacg gaaaggatgg accgtgggtg tagcggcaga cagtgtggtg
     9421 tatcacaagc agggcgccag caccggaaac agtccgaaaa aaagcaaatg ggccatccag
     9481 gcactgcgtt acaagtatcg cggcaagatc ctcttgtaca agaagtatta tcgaaaatat
     9541 ttaccttatt tatatatcca cctgctccgc aggtcgcttc ggtatattct acgaggcaac
     9601 cttcgtgaag ccggagtcat ctatcaagcg atcctgaata cctaagcacc agacatgatc
     9661 atcagccaca agcataagta cgtattcgta gaactgccgc aaaccgcatc cagcgccatt
     9721 gccagagaac tggtagccaa ctatgacgga gaagagatcc ttttcaagca cgcactctac
     9781 cgcacagatt ttctgaagaa ggctactccg gaagaaaagc aataccgggt catcagcgga
     9841 ctgcgcaacc cgatggatat ctgtgtaagt aattatttca agttcaagac cgaccacgag
     9901 aacaggt
//
LOCUS       DAOMAX010000081         9792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_948_length_9792_cov_4.540310, whole genome shotgun sequence.
ACCESSION   DAOMAX010000081 DAOMAX010000000
VERSION     DAOMAX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_9792_cov_4.540310"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK742_05925"
     CDS             <1..1063
                     /locus_tag="PK742_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQU76161.1"
                     /translation="YFSATKINWMLEHVPGARDAAESGDLLCGTIDTWLIWKLTAGAV
                     HATDHSNASRTMLYDLQATGWDPELLGLFGIPAAMMPEIRDSDGFFGNIVLGENKIPI
                     RGVAGDQQAALYGQGCHSAGQAKNTYGTGCFLLLHIGEQAHITDNGLLTTVAWKLDGT
                     ISYALEGSVFQAGSVIQWLRDGLGLLESAEESAKMAAALSDNGGVYLVPAFTGLGAPY
                     WDPDARGMICGLTRGTDRRHVVRAALESIAYQSADILRTMEAAAGIPLSELRTDGGAT
                     GNNWLMQFQSDIIQKPVLIAGIQESTALGAAMLATRHDNAFQQTRSAHQRVCKPVMSA
                     SDSTSLYAGWLAAVKRASS"
     gene            1101..2492
                     /gene="lpdA"
                     /locus_tag="PK742_05930"
     CDS             1101..2492
                     /gene="lpdA"
                     /locus_tag="PK742_05930"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQU76162.1"
                     /translation="MNYDVIVVGSGPGGYVTAIRASQLGMKTAVIEKAELGGVCLNWG
                     CIPTKALLKSAQVFSYMQHAGEYGIQLNGSVQADFNGMIGRSREVAGGMSKGVTFLMK
                     KNKIDVIAGTGMLKPGQNVEVTDAEGKKANYTAKHIIIATGARSRELPALKQDGKKII
                     GYREAMTLAQQPKRMVVVGAGAIGVEFAYFYSTIGTEVTIVEFMDRLVPVEDEDVSKE
                     LARNFKKQGIGVMTSSSVESVDTSGDGCVVKIKTAKGEESITCDVVLSATGVVANIEN
                     IGLEICGIKTEKGKIQVDDFYRTNVPGYYAIGDVVHGPALAHVASAEGITCVEAIAGM
                     HPEPIDYNNLPGCTYCTPEIASVGYTEKAAREAGYEIKVGKFPFTASGKAKAAGAPEG
                     FVKVIFDARYGEFLGAHMIGANVTEMIAEVVTARKLETTGHEILKAVHPHPTMSEALM
                     EATAAAYDEVIHL"
     gene            complement(2537..3886)
                     /locus_tag="PK742_05935"
     CDS             complement(2537..3886)
                     /locus_tag="PK742_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76163.1"
                     /translation="MRYLLLTALSLAVTGSQAQVILGDAINDGILPADDEPICDIPIY
                     PEMLAYTHVMSEDSIVPDFTLFDLDGTAYHLADIMADGKPVVLFGCSYTCYVYRGKVP
                     SINSMVDYFGEDVNFFLVNTVEAHPFGDVSPYFGYEYPGDENIADGVLYPQPTTYGER
                     KAIATDMVANMDLPDIPVLLDDPCNNFWTHFGAFPIPAILINTDGTVYDYHEWLNNYP
                     DNLMASLYTLLDGGIIEDPTPDGTFEMGVYTEECMSGEPGSTIVHHIELINTDTVDAY
                     IGVDRTAEWIPDGWEISLCTDICYPPDVLTTNIYVEAGDTAAFSLYFFTDEMPGEGQT
                     DLYVHQMFNPDINYTITLKACTEEVADTTTAILQAGAASWSVFPNPASGPFTVTVPFI
                     TGNYIIVSVYDLQGNEVFAATTKGQQPLTLNTGLPPGNYILRLASADRQTSTLITLQ"
     gene            complement(3892..5208)
                     /locus_tag="PK742_05940"
     CDS             complement(3892..5208)
                     /locus_tag="PK742_05940"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76164.1"
                     /translation="MSQLSAQVILGPPINVPGLPSADELICDIPLELEVEIEDGPLEG
                     DLVNDFTLYDTEGNQAVLSEVLALGKPVLLISASYSCMVFRGKVALINNIKTERSDEL
                     TVLVVYTVEAHPYGDISPYYGFENTGEQNFEDGVLEPQAVTYGQRVEQAAVMKDALGL
                     QTRVLIDGPCNEWWTANGQAPNNAYLIDTNGIVYDAQAWLDRYPEDIKSSISGLVGED
                     ITGAPVPTGIVGLDSYDEDCVEGEPGSTIIAHVNLSNEDTSASFLDIIRLSEDIPGTW
                     TTSICTDVCYPPETENVDMFVEEGTAASLSFYFYTALTGAEGQANLLIKNIYIPSNSF
                     EVLLKACTELGAPAEVEIVKHEMYLYPNPVAEQLQVFIPGWSGQDIQIRILDLYGRTV
                     HIVPCTLDTIDPVCSVSLQDFPTGSYLIQVMNTHTQESFTETILKH"
     gene            complement(5415..7025)
                     /gene="ftsZ"
                     /locus_tag="PK742_05945"
     CDS             complement(5415..7025)
                     /gene="ftsZ"
                     /locus_tag="PK742_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQU76165.1"
                     /translation="MEFDLPKEQSSIIKVIGVGGGGCNAVNHMFNQGIKDVNFVICNT
                     DSQALDTSPVPNKVQLGPGLTKGRGAGSHPETGKDATMESLKEIKEMLEKNTEMVFVT
                     AGMGGGTGTGGAPVIARTAKEMGVLTIGIVTMPFGFEGKRRREQAERGLAEMRGNVDA
                     ILIISNDKLREIYGNLEFNAAFAKADDILATAAKGIAEIITVPGYVNVDFEDVKTVLR
                     NSGLAIMGSAMAEGEDRALQAVKEALASPLLNDNDISGATNILLNISSGTRPVLMDEI
                     SEITEFVQEAAGNECDIIWGNCNDESLGEHIMVTLIATGFETEKERRQKQQSRNVKRM
                     SLDSDEDAGITVSTEAIIEELEPEPPVVLDEAPETKHPEEEIRVTRLQLDEEKPRQFS
                     FEFTNSFFDEMQNDQMDEDIRQQEEPDSLQFEIRKVEDTEDDEPELEVPARMEHRKPD
                     LADNKDRMRMINQQENERVKRLKSLSLRLNNLDELEKTPAYLRRNVRLEDTPDSAEHH
                     ESKYSLNDSERDGLQIKRNNSFLHDNVD"
     gene            complement(7046..8392)
                     /gene="ftsA"
                     /locus_tag="PK742_05950"
     CDS             complement(7046..8392)
                     /gene="ftsA"
                     /locus_tag="PK742_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQU76166.1"
                     /translation="MGMRIDTNRIVTGLDIGTTKICAIVGRMNEHGKIEVLGMGKADS
                     FGVMRGVVANIDKTVEAIRQAVKEAETKAGVEIREVYVGIAGQHIRSLQHRDILTRND
                     MESEISRDDVNRLIENMHKLVLPPGDRIIHVLPQEFIVDHEPNIKDPVGMCGVRLEAN
                     FHIITGQIAAAKNIFKCVEKAGLKVLDLILEPLASSASVLSMEEKEAGVALVDIGGGT
                     TDIAIFQDGIIRHTSVIPLGGNIITDDIKEGCLIMGNQAEALKVKFGSALATETSDNE
                     IVSIPGIRGREPKEIKVENLARIIQARMEEILEHVYYEIKASGFQKKLIGGIVVTGGG
                     SKMKHLKQLVEYVTGMDTKIGFPVEHLTKSKFQEISHPMYATAIGLILKGFEDERYHE
                     RAAEELSAKKVEAEAQVEVETEDSHEQPARKGGWFNNILTNTRRWFEEDEVNDFNN"
     gene            complement(8383..9171)
                     /locus_tag="PK742_05955"
     CDS             complement(8383..9171)
                     /locus_tag="PK742_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76167.1"
                     /translation="MRVPVKMIRLLRGVALAALTVCLLALTGRQVKKQDDMPCNAVLV
                     GIDTDNGMAFIDSADVLKLLENTLADSLLNRPLHSIPVAEMEAVVLSSPYADQADVYL
                     DAVGNIHVDVVQKQPVARIINKNGVHYYMDEKANKFPVHPTFTCRVPVVTGNISESLS
                     EGDTIQSAELKNAYEIIMYMNTSDLWKAQLEQLQVRGDGVFEFVPKIGGTVVLGKAED
                     LESKLDKLELFYREGLNYTDWDRYSRIDLTYRDQIVCTKKQAWE"
     gene            complement(9168..>9792)
                     /locus_tag="PK742_05960"
     CDS             complement(9168..>9792)
                     /locus_tag="PK742_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQU76168.1"
                     /translation="YAVSTEIEEHGSRVILSNGFDFVLEYPGIHNIENAIAATCVALE
                     LGISEEAVRKALRSFRGIHRRFEVVWKQDGRVLIDDYAHHPEEIRMFINSLRAIYPGK
                     QVEVVFQPHLFTRTRDLATDFAGALDLADHVVLLPIYPARELPIPGIDANTIARHMKG
                     SVRLLESTQLQEFVQDDEHQVIAMVGAGNIDQLVRPMASWLEKKYSR"
BASE COUNT         2408 a         2453 c         2321 g         2610 t
ORIGIN      
        1 ctacttcagc gctaccaaga tcaactggat gctggaacat gttccgggtg ccagagatgc
       61 cgcagaaagt ggtgatctgc tttgtggtac catagatacc tggctgatat ggaaactaac
      121 tgcaggagct gtgcatgcaa cagatcacag caatgccagc cgaaccatgt tatatgatct
      181 gcaagcaaca ggatgggatc cggaattact cggtctgttt ggcattcctg ctgctatgat
      241 gcctgagatc agagactctg acggtttttt tggcaacatc gtgctgggag aaaataagat
      301 ccccattcgc ggtgtggcgg gcgatcagca ggcagcttta tacggacagg gatgtcacag
      361 tgcgggacag gccaagaaca cttatggtac ggggtgtttt ctactgctgc acattggtga
      421 acaggctcat ataactgata acggattgct aaccaccgtt gcctggaaac tggatggaac
      481 catctcctat gctttggaag gaagtgtctt tcaggccggt tctgtgatac aatggttacg
      541 agatggtctg gggctgctgg aaagtgctga agaatctgca aaaatggcag cagcattatc
      601 cgataacgga ggtgtctatc tcgttccggc ctttacgggc ctgggggcac cctactggga
      661 tcccgatgcc agaggcatga tatgcggtct caccagagga acagatcgca ggcatgtggt
      721 gcgggcggcg ctggaaagca tcgcctatca gtctgccgat atactccgga ccatggaagc
      781 agccgcaggt attcccttgt cagaacttcg cacagatggt ggtgccacag gaaacaactg
      841 gctgatgcaa ttccagtcag acatcattca aaagcccgtt ttgattgccg gaatacagga
      901 gagtacagca ctgggagcag ccatgctggc caccagacat gataacgcat tccaacagac
      961 ccgcagtgca catcaacgtg tttgtaagcc ggtcatgtct gcttctgaca gcacctcatt
     1021 gtatgcggga tggcttgctg ctgtaaaacg agccagttcc tgacgcttgc actatctttg
     1081 cagcgctaaa atctcacact atgaattatg atgtgatcgt ggtgggcagt ggccccggag
     1141 gatatgtaac tgctatcaga gcttctcaac tgggcatgaa gaccgcagta atagaaaaag
     1201 ctgaactggg tggtgtttgt ctcaactggg gatgtattcc taccaaagca ctgctcaaga
     1261 gtgcccaggt gttctcatac atgcaacatg ccggcgaata tggcattcag ctgaatggtt
     1321 ctgtgcaggc agatttcaat gggatgatag gtcgcagccg cgaagtggcc ggaggcatga
     1381 gcaagggagt caccttcctg atgaagaaaa acaagatcga tgtgatagcc ggtaccggaa
     1441 tgctgaagcc tggccagaac gtagaggtca cagatgccga aggtaaaaag gcgaattaca
     1501 cagccaaaca tatcatcatc gccaccggag ccagaagccg cgaattacct gcactgaaac
     1561 aggatggtaa aaagatcatc gggtaccgcg aggccatgac cctggcacaa cagccaaaac
     1621 gcatggtcgt agtgggtgcc ggagccattg gtgtagagtt tgcttatttc tacagcacca
     1681 tcggcacaga agtgaccatt gtagaattca tggacagact ggttcctgtg gaagatgaag
     1741 atgtgagcaa agaactggca cgcaacttca agaagcaggg tattggtgtc atgacctcca
     1801 gtagtgtaga gtctgttgat acatccggcg atggctgcgt ggtgaagatc aagactgcca
     1861 aaggcgaaga gtccatcact tgtgatgtag tactaagcgc caccggtgtc gtggccaaca
     1921 ttgaaaatat cgggctggag atctgcggca tcaaaaccga aaagggcaag attcaggtag
     1981 atgatttcta ccgcaccaat gtacccggct attatgccat tggtgatgtg gtgcacggac
     2041 cggcgctggc ccatgtggca agtgcagagg gtatcacctg cgtggaggct attgccggta
     2101 tgcatcctga acctattgac tacaacaacc tgcccggttg tacgtattgt actccggaga
     2161 tcgcttctgt tggctataca gaaaaagcag ccagagaagc cggatacgag ataaaggtgg
     2221 gcaagtttcc ctttactgcc tccggcaagg caaaagcagc aggcgcaccg gaaggatttg
     2281 taaaggtcat ctttgatgcc cgatatggag agtttctggg agcacacatg atcggtgcca
     2341 acgtaaccga gatgatagct gaagtggtaa ccgcccgcaa gctggaaacc accggacatg
     2401 agattctgaa agcggtgcat ccacatccta ctatgtcaga agcactgatg gaagcaacag
     2461 ctgctgctta cgatgaggtc attcacctat aagatcaggc aataaaaaaa gcctgcagat
     2521 atctgcaggc ttttttttat tgcaaagtga tcagggtact tgtttgtcgg tctgcagatg
     2581 caagtcgcag aatatagtta ccaggaggta aaccggtatt caatgtcaga ggttgctggc
     2641 ctttagtggt agcagcaaaa acttcgtttc cctgcagatc atacactgac actattatat
     2701 aatttccggt tataaaagga actgtaaccg tgaatggccc gctagccgga ttcggaaaca
     2761 cagaccatga tgccgcaccg gcctgaagaa tcgcagttgt tgtgtcagcc acttcttctg
     2821 tacaggcttt cagggtaatg gtataattga tatcagggtt gaacatctgg tgaacataaa
     2881 gatctgtctg accttcgccc ggcatttcat ctgtaaaaaa gtacaggctg aaagcggctg
     2941 tatcacctgc ctccacataa atattggttg taagtacatc aggcggataa cagatatcgg
     3001 tacataggct gatctcccaa ccatcaggta tccattcggc agtgcgatcc acacctatat
     3061 atgcatctac tgtatctgta ttgatcagtt caatatgatg cacaatagta gagcccggtt
     3121 ctccgctcat gcattcttct gtataaacac ccatttcaaa agtgccatcc ggggtggggt
     3181 cttcaataat tccaccatcc agcaaagtat ataaagaagc catcaggtta tcagggtagt
     3241 tattcagcca ttcatggtag tcatacactg taccatcagt attgatcagg atggcaggta
     3301 taggaaaggc accaaaatgg gtccagaagt tattgcatgg atcatccagc aacacaggga
     3361 tatcaggcag atccatattg gctaccatat ctgttgcaat agcttttcgt tcaccatagg
     3421 tagtgggctg cggatacagg acgccgtcgg caatgttttc gtcgcccgga tattcatagc
     3481 caaaataggg gctcacatct ccgaaaggat gggcttccac ggtattgacc agaaagaaat
     3541 tgacatcttc accaaaataa tcgaccatag aattgatgga tggtactttt ccgcggtaca
     3601 cataacaggt gtaactgcaa ccaaacagta ctactggttt tccatcggcc atgatatctg
     3661 caaggtgata ggcagttcca tccagatcaa agagggtaaa atccggcact atgctgtctt
     3721 cagacatgac atgtgtatat gccagcattt ccggatatat aggtatatca catataggtt
     3781 cgtcatcagc aggcaatatt ccatcattga tggcatcacc aagaatgacc tgtgcctgag
     3841 aacctgttac ggccaggctc agcgcagtga ggaggaggta gcgcatgaaa tttagtgttt
     3901 aagtattgtt tctgtaaatg attcctgcgt gtgtgtgttc atcacctgaa tcaagtaact
     3961 gcctgttgga aaatcctgca atgaaacgga gcaaaccgga tcaattgtat ccaatgtgca
     4021 gggaactata tgaacagtgc ggccatacag atccagaatt ctgatctgaa tatcctgacc
     4081 agaccatccc ggaatgaata cctgcagctg ctctgccacc gggttggggt agagatacat
     4141 ttcgtgcttt actatctcca cttcagccgg tgcacccaat tctgtacagg ctttaagcaa
     4201 tacctcgaaa gagttggacg gtatgtagat gttcttgatc agcagattag cctgaccttc
     4261 ggcacctgtc agggcggtat aaaagtagaa tgacagtgaa gcggccgtgc cttcctccac
     4321 aaacatatct acattttcag tttcaggcgg gtagcacaca tctgtacaga tactggtggt
     4381 ccaggtgccg ggtatatctt cagacaatct gatgatatcc aggaaagaag cggaagtatc
     4441 ttcattagag agattcacat gtgctatgat agtggagccg ggttcgcctt ctacacaatc
     4501 ttcatcataa gaatccagac ctactattcc tgttggaaca ggtgcacctg taatatcttc
     4561 acccaccaac ccgctgatag acgacttgat gtcttccgga tagcgatcca gccaggcctg
     4621 ggcatcatat acaataccat tggtgtctat caggtaggca ttattaggag cctgtccatt
     4681 ggcggtccac cattcattgc agggtccgtc tatcaaaacc ctggtttgca gacccagggc
     4741 gtctttcatg acagcagcct gctccaccct ctgaccatag gtaaccgcct gaggctcgag
     4801 cacgccatct tcaaaattct gttcacctgt attttcaaac ccataataag ggctgatatc
     4861 accatagggg tgagcctcaa cggtatatac cacgagcacg gtcagctcgt cagaacgttc
     4921 agtcttgata ttgttgatca gggccacctt acctctgaag accatacatg aataactggc
     4981 acttatgagt aagaccggct tgccgagagc gagcacctca gacagaacag cctggtttcc
     5041 ttctgtgtca tataaggtaa agtcattgac cagatcacct tccagggggc cgtcttcaat
     5101 ttccacttcc agttccagcg gaatgtcaca gatcaactcg tctgcagatg gcagacctgg
     5161 cacattgatg gggggaccca gtataacctg agcagacaat tgagacatat tccatgtcac
     5221 aattgctaat aataacacta ataaattctt catagccgta aatgggtgcg ggtcacgagg
     5281 agttacggta tgtttcaaca tttaaagcta acaaaagttt gtctgacctt caaacaacac
     5341 ctgttgttaa tttctaaaaa agaagaaccc cgggaagccg gggttcttcg tgcatactat
     5401 aacagatcat atcatcagtc cacattatca tgcagaaagg aattattcct tttgatctgc
     5461 aggccgtctc tttcgctgtc attcagtgag tacttggatt catgatgttc ggctgaatcg
     5521 ggggtatctt ccagcctgac atttcgccgc aggtaggccg gagttttctc cagttcatcg
     5581 agattattca gccgcaggct cagcgatttc agccttttta cacgttcatt ttcctgttgg
     5641 ttgatcatgc gcatccgatc cttgttatca gccagatcag gcttgcgatg ttccattctt
     5701 gccggtactt ccagttcagg ttcatcatct tcggtatctt ccactttccg aatctcaaac
     5761 tggaggctat caggttcttc ttgctgccgg atgtcttcat ccatctggtc gttctgcatc
     5821 tcgtcaaaaa aagaattcgt gaactcaaat gagaactgcc ttggtttttc ttcatccagt
     5881 tgcaggcggg tcacacggat ctcctcttca ggatgctttg tttccggagc ttcatcaagt
     5941 actaccggcg gttccggttc cagttcttca atgatggctt ctgtgctgac agtgattccg
     6001 gcatcctcat cactgtccag cgacatacgc tttacgttcc tgctttgctg tttctgcctt
     6061 ctttcctttt ccgtttcgaa acctgtagct atgagggtta ccatgatatg ttcacccagc
     6121 gattcatcgt tgcagttacc ccagatgata tcgcattcat tgccggctgc ttcctgtaca
     6181 aactcggtga tctcagagat ctcatccatg agtacaggtc tggtacctga gctgatgttc
     6241 agcagtatat tggtagcacc actaatgtcg ttatcattaa gcaacggaga tgccagcgct
     6301 tctttgacag cctgcagtgc ccggtcttct ccttcggcca tggcagaacc catgatggcc
     6361 agaccgctgt tgcgcagtac tgtcttgacg tcttcaaagt caacgttgac ataccccgga
     6421 acggtgatga tctcagcaat acctttggca gcagtagcca ggatatcatc ggcttttgca
     6481 aaggctgcat tgaactcgag gttgccgtag atctcgcgga gtttatcgtt ggaaatgatc
     6541 agaatggcat ctacatttcc ccgcatttct gccagacctc tttctgcctg ttctcttctt
     6601 cttttgcctt caaagccgaa aggcatggtc acgatgccga tggtcagcac gcccatttcc
     6661 ttggcagttc tggcaataac gggtgcaccg ccggttccgg ttccgccacc catacctgcg
     6721 gtaacgaaca ccatttcggt attcttttcc agcatctcct tgatctcttt gagagactcc
     6781 atagtagcat ccttccccgt ttccggatga gaacctgcac cccggccttt ggtaagtccg
     6841 gggcccagct gcactttgtt gggtacgggg ctcgtatcca gcgcctgaga atctgtattg
     6901 cagatgacaa agttgacatc cttgatcccc tggttgaaca tgtggttcac ggcattgcat
     6961 ccgccaccac caactccgat gaccttgatg atggacgatt gttcttttgg cagatcaaat
     7021 tccataggta tgtgtgttgg ttagcttaat tgttgaaatc atttacttca tcttcttcga
     7081 accacctgcg ggtgttggtg aggatgttat tgaaccatcc accttttctg gccggttgtt
     7141 cgtggctatc ttctgtttct acttccacct gtgcttccgc ttctactttt ttggcactga
     7201 gttcttcagc agcccgttca tggtagcgct cgtcttcaaa ccctttcagg ataagaccga
     7261 tggctgtggc atacatcggg tggctgatct cctggaattt ggatttggtc aggtgttcaa
     7321 ccggaaagcc gatcttggta tccataccgg taacatactc taccagttgt ttcaggtgtt
     7381 tcattttaga gccaccacct gttaccacga tacctccaat aagtttcttc tggaaaccac
     7441 ttgccttgat ctcgtaatac acgtgttcca gtatctcttc cattctggcc tggatgatgc
     7501 gggccagatt ttccactttg atctcttttg gctcgcggcc acggataccg ggaatggaaa
     7561 caatctcatt gtcgctggtc tctgtagcca atgcagaacc aaacttcact ttgagtgctt
     7621 ccgcctgatt tcccataatg aggcagcctt ccttgatatc gtcggtgatg atgttgccac
     7681 ccagcggaat gacagaggta tggcggatga ttccgtcctg gaaaatggct atgtctgtgg
     7741 taccacctcc aatatcaacg agtgcaactc cggcttcttt ttcttccata ctcagcacag
     7801 aggccgatga tgccagcggt tccaggatca ggtccagcac ttttagtcct gccttctcca
     7861 cgcacttgaa aatattcttg gcagctgcta tctgaccggt gatgatgtgg aagttagctt
     7921 ccagccttac accgcacatg ccaaccggat ccttgatatt gggttcatgg tcaacgatga
     7981 actcctgtgg cagaacatgt atgatgcgat cgcccggggg aagcacgagc ttatgcatgt
     8041 tctcgatcag ccggttcacg tcatctctgc tgatctcact ttccatgtcg ttcctcgtca
     8101 ggatatcccg gtgctgcaga ctgcggatat gctgccctgc aattcccaca tatacttcgc
     8161 ggatctccac accagctttt gtttcagctt ctttcaccgc ctggcgaatg gcttccactg
     8221 ttttgtcaat gttggctacc accccacgca tcacgccaaa gctgtcagcc ttgcccatgc
     8281 ccagcacttc gatcttgccg tgttcattca tccggccaac gatcgcacag atcttggtgg
     8341 taccgatatc gagtccggtc acgatcctgt tggtgtctat tctcattccc atgcttgttt
     8401 tttagtacat acgatctggt cccggtacgt caggtcgatc ctgctgtatc ggtcccagtc
     8461 tgtatagttc aatccctcac ggtaaaacaa ttccagtttg tccagctttg attccaggtc
     8521 ttctgccttt cccagcacaa cggtaccgcc aatcttgggc acaaattcaa atacaccgtc
     8581 acccctcacc tgcagctgtt ccagctgtgc tttccacagg tcagaggtgt tcatatacat
     8641 gataatttcg tatgcgtttt ttagttcggc agactggatg gtatcacctt ctgagagtga
     8701 ttcgctgatg tttcctgtta ccaccggtac acgacatgta aatgtagggt gaacaggaaa
     8761 cttattagcc ttttcatcca tgtaataatg cacaccgttc ttgttgataa tacgtgctac
     8821 aggttgcttc tgcactacat ctacgtggat gttcccaaca gcatccagat aaacatcagc
     8881 ctgatctgca tacggactgg acaataccac cgcttccatc tcagctaccg gtatgctgtg
     8941 caagggtctg ttcaggagtg aatcagcaag ggtattctcc agcaatttca gcacatctgc
     9001 actgtctatg aacgccatcc cgttatccgt gtcaatgccc accaatacag cgttacaggg
     9061 catatcatcc tgctttttta cctgcctgcc ggtcaatgcc agcaaacaga ccgtcagggc
     9121 agccagcgct accccgcgta acagtctgat catttttaca ggcaccctca tctgctgtat
     9181 tttttttcca gccaggaagc catgggcctg accagttgat caatatttcc tgcccccacc
     9241 atagcgatca cctgatgttc atcatcctgt acaaattcct gtagttgtgt ggattcaagc
     9301 aatcgaacac tgcctttcat atgtctggca atggtatttg cgtctatgcc gggaatgggc
     9361 aattctcttg ccgggtatat gggtagcagc acaacatggt ctgccagatc aagtgccccg
     9421 gcaaagtctg ttgccaggtc tctggtccgg gtgaacaagt ggggttgaaa taccacctca
     9481 acttgctttc ccggatagat ggcccgcaag ctgttgatga acatgcgaat ctcttcagga
     9541 tgatgcgcat agtcatctat aagtaccctt ccgtcttgct tccacaccac ttcaaagcgg
     9601 cgatgtattc ccctgaacga ccgcaatgct tttctgacag cctcttctga aatgcccagt
     9661 tccaaggcta cacaagttgc tgcaatggca ttctctatgt tgtgaatacc cggatactcc
     9721 aggacgaaat caaagccatt gctgagaata acccggctgc catgctcttc gatctctgtg
     9781 gatacagcat ac
//
LOCUS       DAOMAX010000082         9677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_966_length_9677_cov_4.163583, whole genome shotgun sequence.
ACCESSION   DAOMAX010000082 DAOMAX010000000
VERSION     DAOMAX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_9677_cov_4.163583"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PK742_05965"
     CDS             <1..1095
                     /locus_tag="PK742_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HQU76169.1"
                     /translation="PAATELEQRMMEWLRDLCGLPSHWQGVLQDTASTATLTAVIAAR
                     EKKTTHRVKNDGFSGLPKMILYTSEHAHYSVEKAAMSAGIGRNNVRKIQCNADLSMDT
                     DALRMQVEADLQDGHLPFMVCATLGTTSTLAFDHLQEIAAVCNAFDLWLHVDAAYAGS
                     AFILEEERGVMAGIGAADSYVFNPHKWLFTQFDCSAFYVSDPDDLLTVFSVSAEYLKA
                     TREQGIINYKDWGIPLGRRFRSLKLWYVLRTFGRKGLQDKISRHLELARTLQQRLTAM
                     DGWIMPYPMHMNVLCLQYRPERLKTLAEWNEVTRNILDEINSDGRFYLTGTTVEEMFL
                     IRIVPAQTEVSRDTIEALAELIEEITMRCA"
     gene            complement(1076..2473)
                     /locus_tag="PK742_05970"
     CDS             complement(1076..2473)
                     /locus_tag="PK742_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQU76170.1"
                     /translation="MGWGKVFSEKRRFFHFHGTRLPVWSTGYWIAQAKLVGITYTLYH
                     SKFQEVGWKEHVPDDKPILFAISHRNAFMDSLAFVHIHDSQVWQLARGDAFKNPLMRK
                     LFYFFHMLPIWRERDGVDTREANEETFNACYDILALNGMVGIYPEGDCINERHIRPLK
                     KGICRIAFGAMEKYNWEIDLHIVPVGVSYTDAEKFGKRQLIQFGPAVRVQTYKEQYQK
                     NSAAAINTLKDELQLRMQDQVVHIPRSHNHHDIDQAVVIGARAFLKEEGSPYTPIAAL
                     RKEQELSKKLETIRLNDHEGISHLAKTLHKYVKGRDALDLRENTFDQQRQSMGYRVGM
                     LLVLLLTFPVFITGWITQIIPFRLPQWIVRKNIKQRIFFSSLQYAIGLFTFTFFYLLY
                     FILAWVLSGHFWMAAILLLVLPVWGKSTYLWWRAYKRWLSVVRLKIKLRRKDDTAVHM
                     MADREELLRIMRNAS"
     gene            complement(2499..3428)
                     /locus_tag="PK742_05975"
     CDS             complement(2499..3428)
                     /locus_tag="PK742_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HQU76171.1"
                     /translation="MLVEAVQIDEIRLHFDRDNLFLLNFILAFLMYGIALNLTIEDFR
                     NLIRQPRKAVAGVLSQWVIMPLLTYILVLVMQPSPSIALGMFLLAACPGGNMSNYLSS
                     IARGNVALSVTLTAASTVLCIFMTPINFTLYGSLYGPTRELMHEISVEPGELFQAVFI
                     ILGVPLVLGMLTARFLPRFTAIIHKPVRWLSLIIFISFIVFAFLANYAIFLEVIGIIF
                     ILVLLHNGLGLAAGYTVASVFRMDFADKKCLSIETGIHNAALGLILIFSFFSGLGGMA
                     VVAGWWGIWDLVSGFALAGFWWWRHERKAASVS"
     gene            3629..5758
                     /locus_tag="PK742_05980"
     CDS             3629..5758
                     /locus_tag="PK742_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQU76172.1"
                     /translation="MKSHDSTHIKNVVLVGHSGSGKTTLAECMLFEAGVINRRGTIAE
                     KNTIGDYSDIEHERGNSIFSHIMHVDWKDTKINIIDTPGMDDFVGEVISSFKVADTGI
                     MVINAANGVEVGTEIIWEYADHLKKPMLFVANHLDEEKSDFDAMVEQAKDRFGRKVTV
                     VQYPYNQGVDFNAVIDVLNMVMYRFGADGGKPEKLDIPAEEMDKAKQLHNDLIEAIAE
                     NDETLMERYFEKGTLDETDMQKGLLISIRDHELFPMFCISAKHNMGSGRIMGFIDYCC
                     PSAAHMNPEKLTNGADLPCNSSNPLVAFVFKTMSEPHLGDMSFFKVCSGKLKVGDDLV
                     NTANGQTERLSQIYLVQGKKREPVEEVLAGDIAATVKLKKTHTNNTLSAKGQNVEVVP
                     IHYPEPKVQAAIHVHNKGDEEKMGMALNHIHEEDPTITIDHNMELKQTIINAQGEMHL
                     AMLKWKIEHLYGIQFDYEKTRIPYRETIQKSVRSDYRHKKQSGGAGQFAEVHMLVEPW
                     YEGMPDPPDLNVRGREEHPLKWGGKLVYYNCIVGGSIDARFLPSILKGVMDKMENGPL
                     TGSYVRDVRVSVYDGKMHPVDSNDMAFKLAGMMAFRNAFREADPKILEPVYDLEIMVP
                     ADMMGDVMGDLQTRRGIVMGMDSDGHYQKIKARVPLSELHDYSSTLRSFTQGRGKFTR
                     QFAEYAPVPFDLQQKLIEAHQEELAEA"
     gene            complement(5820..6146)
                     /locus_tag="PK742_05985"
     CDS             complement(5820..6146)
                     /locus_tag="PK742_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HQU76173.1"
                     /translation="MGKFVVSTRANGEIQFNLKADNGQVILSSEGYKSKDACMNGIES
                     VRKNSATEARFEKLTAKNGKFYFNLKATNGQVIGSSQMYEAEASRDNGIASVMKNAPD
                     AGVAEE"
     gene            complement(6201..7067)
                     /locus_tag="PK742_05990"
     CDS             complement(6201..7067)
                     /locus_tag="PK742_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76174.1"
                     /translation="MPVTNPLLRYSAAATAFLAGAIPVQGQMVFTDVDPDLILPVGDS
                     YDLDLNNDGTIDFVISHQESTAPSFFYVSSSFSPDARMEHGLVQPLSLNSVAGQLPGL
                     LYAYQLPVDYLVGYDLNFHQETAQLFYKLQAVDYPAPGSLFTVSSEGNFQAETEGFVP
                     LNFRIDDAVRFGWVRLAVNADMDIVVMSYAYEATVEEPAITFIDTTQAIQPAAFLEPT
                     VYAAGEMLFIDLSEVTEQAIYTIHDLGGRHLLEGTVSGQGLYSRTLSLPAGVYVITLT
                     WEGQRYVRRIFL"
     gene            complement(7188..7442)
                     /locus_tag="PK742_05995"
     CDS             complement(7188..7442)
                     /locus_tag="PK742_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQU76175.1"
                     /translation="MVITFEELRHIKDALPHGSMERIAEELNINIDTVRNYFGAEHFE
                     SPEKIADVHFEKGASGGVVQLESTDILEAARRILQEQAVH"
     gene            complement(7568..9139)
                     /locus_tag="PK742_06000"
     CDS             complement(7568..9139)
                     /locus_tag="PK742_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76176.1"
                     /translation="MKQAWKWTLSLLFMPAALMAQSTDVNELPPLDELGEGKFMGYTG
                     GLYPEGRNIMPAGFFIEAVNISRSIQPLNKQGSPDPNGQIGVVAIGASTVAMFGDALG
                     KMIYDVPGIRPEIVYVNGGIGGQDLNKIHDPMARYWLEVDKRVQLAGLTNEQVQVAWM
                     QEDDLRNTTAKFPDRADMLADAFTYAAQQLKIRYPNLQLLYFTGRHTTEYMPADAKVK
                     HMEPRAYLNGWGTKFVIERQINGDPELSFKGPDAKAPLLLWGPYFWTQGEKPRADGYT
                     FTKDLVNADGVHPNASGEVKVARDILDFWQQDPVSVQWLTGGEAVADEPSSGFLVKYL
                     NDVLAEIPHSRLSGDVLLLLLKDAEVRMKSSANGQDGYISFPRLDDGMYTWVLVDQGD
                     FIQRGDFLIKDGQRVFRKEESPEKDTSVDEVRDDRSAYVSKNDVPEDQPAWFLNGSNK
                     LPKLIRLLGGDKWAKAVFTTPDGEVVLVVEDVLHKHTRVNDILPPGNYILKFYDENGD
                     QINVDREIPEYLRLK"
     gene            complement(9182..>9677)
                     /locus_tag="PK742_06005"
     CDS             complement(9182..>9677)
                     /locus_tag="PK742_06005"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HQU76177.1"
                     /translation="PPEAQKIENTLRDLGLNKMCDDVILSINQAAEKAAIEAKPILIN
                     SIRQMTVQDAMDILFGANNAATNYLEKTTTTQLTEKFTPVIDNALASVNATKYWGDAV
                     NYYNQIPLVQDLNPDLTGFVTQKALEGLFLMIEQEELKIRQDPGARAAQIVKDVFAYY
                     DANK"
BASE COUNT         2536 a         2450 c         2375 g         2316 t
ORIGIN      
        1 ccagctgcta ctgaactgga acagcggatg atggaatggc ttcgagatct gtgcggattg
       61 ccctcccatt ggcagggtgt gttacaagac acggcctcca ccgctacgct gaccgctgtc
      121 attgcagcca gagaaaagaa aacaacacat cgggtaaaga acgatggatt ttccggtctg
      181 ccgaaaatga tcttatacac aagtgaacat gcacattact ctgtagaaaa agctgccatg
      241 tctgcaggaa ttggcagaaa caatgtgcga aagatccaat gtaatgctga tctctccatg
      301 gatactgatg cactgcgcat gcaggtggaa gccgatctgc aggatggtca cctgccgttc
      361 atggtctgcg ccacgctggg cacaaccagt acgctggcgt tcgatcattt acaagagatc
      421 gcagctgttt gcaatgcctt tgacctttgg ttgcatgtgg atgctgccta cgcaggcagt
      481 gcctttattc tggaagaaga acgcggtgtg atggcgggta tcggggctgc cgacagttat
      541 gtattcaatc cgcataagtg gttatttacc cagttcgatt gttccgcttt ttatgtgtct
      601 gatccggatg atctgctaac cgttttcagt gtttctgcag aatacctgaa agctacccgc
      661 gagcagggca ttatcaatta caaggactgg ggtattccac tgggaagaag atttcgatca
      721 ctgaaactct ggtatgtatt acggacattc gggaggaagg gtttgcagga caagatctca
      781 cggcatctgg agcttgcacg aaccctgcaa caaagactga cggccatgga cggctggatc
      841 atgccttacc ccatgcatat gaatgtgttg tgcctgcaat acaggccgga gagactgaaa
      901 acattagcag aatggaacga agtgacccgg aatatcctgg acgaaataaa cagcgacgga
      961 cgattttatc tgaccggaac cacggtggaa gagatgtttc tgatacgtat cgtaccggca
     1021 caaacagagg tgagcagaga tacgatcgaa gcgctggctg aactgatcga agagatcacg
     1081 atgcgttgcg catgatgcga agtaattctt cccggtctgc catcatgtgt acagcagtat
     1141 catccttccg gcgcaacttg atctttagtc gaacgactga caaccaccgc ttgtaagccc
     1201 tccaccacag ataggtggac tttccccaaa caggcagcac cagtaacaag atagcggcca
     1261 tccagaaatg accggagagc acccatgcaa gaataaagta caacaggtag aaaaaagtaa
     1321 atgtgaacag cccgatagcg tattgcaaag aggaaaagaa tatacgctgt ttgatgttct
     1381 tcctgacgat ccattgaggc aggcggaaag ggatgatctg cgttatccat cctgttatga
     1441 agaccggaaa agtgagcagc aataccagga gcatgccaac acggtaaccc atgctttgtc
     1501 tctgctgatc gaacgtattc tctctgagat caagtgcatc cctgcctttc acatacttgt
     1561 gcaaggtctt cgccaggtgg ctgataccct cgtgatcatt cagcctgatc gtttccagtt
     1621 tcttgctgag ttcctgctct ttccgcagcg ctgctatcgg agtatagggt gacccttctt
     1681 ctttcagaaa agcccttgca ccaatgacca ctgcctgatc gatgtcatga tggttatggc
     1741 tgcgtggaat atgcaccacc tgatcctgca tccggagttg cagctcatcc ttcagcgtat
     1801 tgatggctgc cgcactgttc ttctgatact gttctttgta ggtctgtacc cttactgccg
     1861 ggccgaactg aataagctgt cgcttgccga atttttctgc atcggtataa ctcacgccaa
     1921 ccggcacgat gtgcaggtct atctcccagt tgtatttttc catcgcaccg aaagcaatgc
     1981 ggcagatgcc tttcttgagc ggacggatat gtcgctcgtt gatgcaatcg ccctccggat
     2041 agatgcccac cataccattg agcgccagga tatcatagca ggcgttaaag gtctcctcat
     2101 tggcttccct ggtatccacc ccatcccgct cacgccatat gggaagcatg tggaagaagt
     2161 agaacagctt gcgcatgagg ggattcttaa aggcatcacc ccttgccagc tgccagacct
     2221 gtgagtcatg gatatgcaca aacgccaggc tgtccatgaa agcattacga tgactgatgg
     2281 caaacaggat cggtttgtca tccggcacgt gttccttcca gccaacctcc tgaaatttac
     2341 tgtgatagag tgtataggta atgcccacca gtttggcctg tgcgatccag tatccggtac
     2401 tccagacagg cagcctggtt ccatgaaaat ggaaaaagcg gcgtttttcg ctgaatactt
     2461 taccccagcc cataaaaagt tgtgcaaact tagaaaaatc aactgacgga ggctgccttc
     2521 cgttcatgcc gccaccacca gaagccggcc agggcaaacc cactcaccag atcccagatg
     2581 ccccaccatc ctgccaccac ggccatacca cccaggccac tgaagaaaga aaagatcagg
     2641 atcagtccga gagcagcatt atgaataccg gtttctatgc tcaggcattt cttatctgca
     2701 aagtccatgc ggaatacaga tgcaacagta taaccagctg ccagtccgag gccattgtgg
     2761 agcaacacca gaatgaaaat gataccgatc acttccagaa aaatggcata gttggccaga
     2821 aatgcaaaga ctatgaaaga gatgaagatg ataagcgaca accagcgtac cggcttatgt
     2881 atgatagccg taaagcgggg cagaaagcgg gcggtcagca tgcccagcac caatggtaca
     2941 cccaggatga tgaatactgc ctggaagagt tcgccgggtt ctacgctgat ctcatgcata
     3001 agctcccggg tcgggccata gaggctgccg tataaggtga agttgatggg agtcatgaaa
     3061 atacacagca ccgtagaggc agcagtcagg gtaacagaaa gcgccacatt accccgggcg
     3121 atgctgctca ggtagttcga catattaccg ccggggcagg ccgccagcag aaacataccc
     3181 agtgcaatgc tgggagaagg ctgcatgacc agcactaata tataggttag caatggcatg
     3241 atgacccact gcgacaatac gcccgcaaca gcttttctgg gctgacggat caggttcctg
     3301 aaatcctcaa tggtcaggtt cagggctata ccatacatga gaaatgccag gatgaaattg
     3361 agcaggaaga ggttatcccg gtcaaaatgc agcctgatct catctatctg aacggcttcc
     3421 acaagcatgt gcaatgataa ttcattaatg agtgaaagag caaataggcc gatctggcaa
     3481 cctgtgtgca caatagtgct tcagctcctg tttaaaccgc tcaaaaaaca ggttaaaaac
     3541 taatctttcc tcttgtttgc gcaggtcata ttccctaatt tgcaagcctg caattgatca
     3601 ttcacttaaa ccataaagga ggaatgttat gaagtcacac gatagcaccc acatcaaaaa
     3661 cgttgtgctg gttggtcatt ccggttccgg gaagacaaca ctcgctgagt gtatgctttt
     3721 cgaagccggg gtgatcaaca gaagaggaac catcgctgag aagaacacca ttggtgatta
     3781 cagcgatatt gagcacgaaa ggggaaacag catcttcagt cacatcatgc atgtggactg
     3841 gaaagacacc aagatcaaca tcattgacac accgggcatg gacgacttcg taggtgaggt
     3901 catttcgtca tttaaggtgg cagataccgg tatcatggtc atcaacgcag ccaatggcgt
     3961 ggaggtgggt accgagatca tctgggaata tgcagaccac ctgaaaaagc ccatgctctt
     4021 tgtggccaac cacctggatg aagaaaaatc agatttcgat gccatggtag agcaggccaa
     4081 agatcgcttt ggaagaaagg tcaccgtggt acaatatccg tacaaccagg gtgttgattt
     4141 caatgcggtc attgacgtgc tgaatatggt catgtaccgt tttggtgccg atggagggaa
     4201 gcctgaaaaa ctggatattc ctgccgaaga aatggacaaa gcgaagcagc tgcacaatga
     4261 cctgatagaa gccatcgccg aaaacgatga gacactgatg gaacgctatt ttgaaaaagg
     4321 caccctcgat gaaactgaca tgcagaaagg gttgctcatt tccatccgcg atcatgagct
     4381 gttcccgatg ttctgcatct ctgccaagca caatatgggc agcggccgca tcatgggatt
     4441 cattgattac tgctgcccga gtgcagcaca tatgaatccg gaaaaactga ccaacggagc
     4501 agatctgccc tgtaattcat ccaacccgct ggtggcattc gtattcaaga ccatgagtga
     4561 accccatctg ggtgatatga gcttcttcaa agtttgctca ggcaaactga aagtgggcga
     4621 tgacctggtg aacaccgcca acggtcaaac cgaacgcctc tcacagatct atctggtaca
     4681 gggcaagaaa cgggaacctg tagaagaggt gctggccggt gatattgccg ccaccgtcaa
     4741 actaaaaaag acacatacca acaataccct gagtgccaag gggcagaatg tggaggtggt
     4801 acctattcat taccccgaac ccaaggtgca ggctgccatt catgtgcata ataaaggcga
     4861 tgaagaaaag atgggtatgg cgctcaacca tattcatgaa gaggatccta ccatcaccat
     4921 agaccacaac atggagctca aacagaccat catcaatgcc cagggcgaga tgcacctggc
     4981 catgctgaag tggaagatcg agcacctgta tggcattcag ttcgattatg aaaagacccg
     5041 cataccctac cgggagacca tccagaaatc agtgcgcagc gactaccgtc ataaaaagca
     5101 aagtggtggt gccggacagt tcgcagaagt gcacatgctg gtagaaccct ggtatgaagg
     5161 catgcctgat ccgcccgatc tgaatgtccg tggtcgggaa gaacatcctt tgaaatgggg
     5221 aggcaaactg gtgtattaca actgcatcgt gggaggttcc attgatgccc gtttccttcc
     5281 gtccatcctg aaaggggtca tggacaagat ggagaatggt ccgcttaccg gttcctatgt
     5341 gcgtgatgtt cgtgtgagtg tgtatgatgg taagatgcac ccggttgaca gtaatgatat
     5401 ggccttcaaa ctggccggca tgatggcatt ccgcaatgcc ttccgcgaag ctgatcccaa
     5461 gatcctggaa ccggtatatg acctggagat catggtacct gccgatatga tgggcgatgt
     5521 gatgggcgac ctgcagacac gtcgcggcat tgtgatgggt atggacagcg atggccatta
     5581 tcagaagatc aaggcacggg ttccgctgtc agagttacat gactattcca gcacacttcg
     5641 cagtttcacc cagggaagag gaaagttcac ccggcagttt gcagagtatg ccccggttcc
     5701 ttttgacctg cagcagaaac tgatcgaagc acatcaggaa gaacttgcag aagcctgaca
     5761 agttgcagat ataaaaaagg cgctctacag agcgcctttt cttttttcat tcagtccgtt
     5821 cattcttctg caactcctgc atcaggtgcg ttcttcatga cactggcaat gccattgtct
     5881 ctgctggctt cagcttcata catctggctg gaaccgatca cctggccgtt ggtggcttta
     5941 aggttgaaat agaacttccc gttcttggcg gtcagttttt caaaacgtgc ttccgtagca
     6001 ctgttcttgc gaacactttc aatgccattc atgcaggcat ctttggactt atagccttcg
     6061 ctggacagga tgacctgacc gttgtcagcc ttcagattga actggatttc cccgttggca
     6121 cgggttgaaa caacaaattt tcccatgata ataaagattg ttggttatgc tggctctaag
     6181 ctacaggata agaccaagac ctacaagaat attctgcgca cataccgctg tccttcccag
     6241 gtcagtgtga tcacatacac tcctgcaggc aatgacaaag tacgggaata caaaccctga
     6301 ccggacactg ttccttccag caaatgtcgt ccacccaggt catgaatggt ataaatggct
     6361 tgttcagtca cctcactgag atctatgaac agcatctcac ctgcagcata caccgttggt
     6421 tccagaaatg ctgccggctg tattgcctgc gtggtgtcta taaaagtaat ggccggttct
     6481 tccactgttg cttcgtatgc ataactcata acaacaatgt ccatatctgc gttcacagcc
     6541 aggcgtaccc atccaaaccg cactgcatca tcaatacgga aattgagtgg tacaaaccct
     6601 tctgtttcag cctgaaaatt cccttcactg gaaacggtga acaaggagcc cggagccggg
     6661 tagtcaaccg cctgcagttt atagaataac tgagctgttt cctgatgaaa gttgagatca
     6721 tagccaacca gataatcaac cggcagctga taagcataca ataaaccggg cagctgacct
     6781 gccactgaat tgagtgacaa aggttgtacc agaccgtgct ccattctggc atccggactg
     6841 aaagaactcg acacatagaa aaaagacgga gctgtagatt cctggtgact gatcacaaaa
     6901 tcgatggtgc cgtcattgtt cagatccaga tcataggaat ctccaacagg cagaataaga
     6961 tcagggtcca catcggtgaa taccatctga ccctgcacag gaatggcacc ggccagaaag
     7021 gcggtagctg cagccgaata tcggaggaga gggttggtga ccggcatgcc gcaaaagtaa
     7081 ccaaaaccga attggtgaac gatgacttat gttaggcagg cagagggaat gaaaaaggcc
     7141 ctgtccggta aaagacaagg ccaggttcaa gtatgaaaag agagaagtta atggacagcc
     7201 tgttcctgca ggatacgcct ggctgcttcc agaatgtctg tgctctcgag ctggacaaca
     7261 ccaccgctcg cacctttctc aaagtgcacg tcggcgatct tctccggcga ttcgaaatgc
     7321 tctgccccaa aatagttacg gaccgtgtcg atattgatgt tcaattcttc cgcgatccgt
     7381 tccatactac cgtgtggcag tgcatccttg atatgcctga gttcttcaaa agtgatgacc
     7441 ataaacctga tgttttgttg aacaatagaa gtgcacctaa taatacagcg cttctccggt
     7501 ttttcaaaat tttttaaccc ggatccggga aaaaatcaag tcacaatacc ggctttctcc
     7561 gttgagatca cttcaggcgc aggtattccg gaatttctct gtccacgttg atctgatctc
     7621 cgttctcatc ataaaacttc aggatgtagt ttcccggagg cagtatgtca ttgaccctgg
     7681 tatgcttgtg cagtacgtct tccaccacca gcacgacctc gccgtcgggt gtggtgaaca
     7741 ctgccttcgc ccatttatcg ccacccagca atctgatcag tttgggcaat ttgttggatc
     7801 cgttcaggaa ccaggcgggc tgatcttcgg gtacatcatt cttagacaca taagcggagc
     7861 ggtcatcacg aacctcatct accgaggtat ccttttccgg agattcttct ttgcggaata
     7921 cccgctgacc gtctttgatc agaaaatcac cccgctggat gaaatcaccc tggtccacca
     7981 gcacccaggt gtacatgccg tcatccagtc ttggaaaaga aatatagccg tcttgtccgt
     8041 tggcactgct cttcatgcgt acctctgcat ctttcagcag cagcagaagc acatcgccgg
     8101 aaagtctgct atggggtatt tcggcaagta catcatttaa atatttcacc aggaaaccgg
     8161 aagaaggttc atctgccact gcttcgcctc ctgtgagcca ttgcacagat accggatcct
     8221 gctgccagaa atccagaatg tcgcgggcca ctttcacttc gccagatgca ttggggtgta
     8281 caccgtcagc attgaccagg tcttttgtaa aagtgtaacc atcggcacga ggtttttctc
     8341 cctgcgtcca gaagtacggc ccccacagca ataagggcgc tttcgcatcc ggacctttaa
     8401 aggaaagttc cggatcgcca ttgatctgtc tttctatcac aaattttgtt ccccatccgt
     8461 ttagatatgc ccgtggctcc atgtgcttga ccttggcatc ggcaggcatg tattctgtgg
     8521 tatgccgtcc ggtaaagtac agcaactgca ggttcgggta gcggatcttc aactgctgag
     8581 cagcataggt gaaagcatct gccagcatat cagcccggtc cggaaatttg gcagtggtgt
     8641 tccgcaggtc atcttcctgc atccaggcta cctgtacctg ttcattggtc aaaccggcaa
     8701 gttgcacacg cttgtccact tccagccagt agcgggccat cggatcatgg atcttgttga
     8761 ggtcctgccc tccaatacct ccgttcacat acacgatctc cggcctgata cccggcacat
     8821 catagatcat cttccccagc gcatctccga acatggccac cgtgctggct ccaatagcca
     8881 ccacgccgat ctgcccgttt ggatccggtg atccctgttt attcaggggc tgaatggacc
     8941 gtgaaatatt gactgcttca ataaagaaac cggctggcat gatattgcgt ccttccggat
     9001 acaatccgcc cgtatatccc atgaacttgc cttctcccag ttcatccagc gggggaagtt
     9061 cattgacatc ggttgactgc gccatcaggg cagcaggcat aaaaagcagg gataatgtcc
     9121 atttccaggc ttgtttcatc ttggttcttt actatttata catgaaagtt ccgtgccaga
     9181 atcacttatt cgcatcataa taggcgaaca catccttgac gatctgcgcc gcacgggcac
     9241 ccgggtcctg tctgatcttc agctcttcct gttcgatcat cagaaataac ccttccagcg
     9301 ccttctgtgt tacaaatccg gtcaggtccg gattgaggtc ctgcaccaac ggtatctggt
     9361 tgtaatagtt gaccgcatca ccccagtatt tggtggcatt cacactggcc agagcattgt
     9421 caatgacagg tgtgaacttt tcggtcagct gggtagtggt ggtcttttcc agatagtttg
     9481 ttgcagcatt gttcgcaccg aacaggatat ccatcgcatc ctgcacggtc atctggcgga
     9541 tggaattgat caggatgggc ttggcctcta tagccgcctt ctctgctgcc tggttgatgg
     9601 acagtatgac atcatcacac atcttgttca gtcccaggtc acgaagggta ttctcgatct
     9661 tctgagcctc cggtggg
//
LOCUS       DAOMAX010000083         9513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_994_length_9513_cov_4.253648, whole genome shotgun sequence.
ACCESSION   DAOMAX010000083 DAOMAX010000000
VERSION     DAOMAX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_9513_cov_4.253648"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PK742_06010"
     CDS             complement(<1..968)
                     /locus_tag="PK742_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HQU76178.1"
                     /translation="MTGWLIFIAVLLVLIVLFQVTRTLDLVSQLRGDDDSQLEQTSRM
                     QAIALFIFMALGVIGFFWSFRHFAPTNVDVASEHGELIENMFIATLVVTSIVFVLCNV
                     LLFTFVLMYRYKKGREAVHFAHSNKLEFFWTVVPTIVLTGLVVFGLQAWTRIMGPPEE
                     DAVIFEMTGQQFFWTSRYPGADGKLGPRDYSLICAENPLGIVTREYVEHRINTLKGNA
                     ELGVEGEIAALENRKSELPGLIAALEDKIAKRPNRYMKDQLQEELDALEDEMDEIDDH
                     ILRRNQTLDRITKKYTEDYYMANAEALTWGYDDFLPSELHLPVNHGA"
     gene            complement(986..2359)
                     /locus_tag="PK742_06015"
     CDS             complement(986..2359)
                     /locus_tag="PK742_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76179.1"
                     /translation="MSSTTKFDFTSTHKRNFIILFVIGMMAVLTGVFTGQADAQKTWA
                     NLLVNAVFFTGLSALAVFFLAAHQIAFAGWHALVKRVPEAMTSFLWIGAICILVVTGG
                     TIGNYNNLYHWSDTFLIKEKVTMAELEAYEKEHAHGHEMEGDHESGEHHGEEHSMLSN
                     GTVVLASAGSEGGESGMIDNPYYDEILDGKSAYLNDAFFMTRTIIYLVLWVFIGYQLR
                     RFSIKEEETGEKMWFQKTRTWAAVFLVVWAVSSSMMAWDWVMSLDPHWYSTLFGWYNF
                     ISLWVASLSVVILLIIYLKRNGYMPQTNENHLHDLGKYIFGFSVFWTYLWFSQFMLIW
                     YGNIPEETAWFLERKREYNFLFGANLAVNFLVPFLVLMRRDAKRNFTVLTVVASLLIV
                     FHWVDFYLMIIPSTVHGAAPIGYFEIGMALAFAGVFLYSTFRALSKVSLVPQHNPFIQ
                     ESIEHHI"
     gene            complement(2374..3039)
                     /locus_tag="PK742_06020"
     CDS             complement(2374..3039)
                     /locus_tag="PK742_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQU76180.1"
                     /translation="MKRSYFLILMAAAVLTGLQACKKASGDYPGDSYTWDMMYSKAYE
                     PYALNPLMPDSVGAMLPVNGTIPYPGNAISGQMDPALASISLPYEYAKTDEDYERAGL
                     ELINPLQATPENIAAGQHAFNNMCAVCHGTEGNGKGVIVASGKYGAVPPSYFDAGYID
                     MPDGKMFHSITYGKNAMGSYAYALTKKERWQIILYINSMQDAWLASNQPAQTQVAETD
                     VNP"
     gene            complement(3051..3581)
                     /locus_tag="PK742_06025"
     CDS             complement(3051..3581)
                     /locus_tag="PK742_06025"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HQU76181.1"
                     /translation="MSGKKVLFGLFDDEHVLLDAVKGVRAEGFKITEVMTPFPVHGLD
                     EAMGLRMTKLHSAGFLFGATGTLTALGFITWISTVNYPINYGGKPYFSLPAWIPITFE
                     LTVLFAAVGMTLTYLYLNRLAPGIKHRILDERITSHMFAMSFEIDADTSDARKAEIRK
                     ALEANGAVEIHEQDLD"
     gene            complement(3583..5016)
                     /gene="nrfD"
                     /locus_tag="PK742_06030"
     CDS             complement(3583..5016)
                     /gene="nrfD"
                     /locus_tag="PK742_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQU76182.1"
                     /translation="MFVSPIREPLIQGHKTYAQMSDDIIGPVEAKPTKTWMLAVTCTA
                     LLAITGFVMIGLTITYGIGLWGLNKTIGWAWDITNFVWWIGIGHAGTLISAILLIFRQ
                     KWRTGVNRSAEAMTIFAVMCAGLFPLIHMGRIWLAFFIFPYPNTRGPVWSNFNSPLLW
                     DVFAISTYFTVSLLFWYVGLIPDLATVRDRAKTAFRKKIYNVFSFGWTGTAKQWQRFE
                     SLSLVLAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVLTLM
                     LVVRKIFHLEDYITLSHIESMNKIIILTGSIVGVAYITELFTAWYSQNQYEQYAFINR
                     SMGPYAWSYWGMMTCNVISPQLFWFRKLRRSVVFTFFMSIVINIGMWFERFVIIVTSL
                     HRDYIVSSWSYYTPTYVEVFIYLGTIGFFMTLFLIFAKVAPVIALAEVKSILKSAGDQ
                     YIGEHAAHAHHTEDAGHGHYETLIKPQ"
     gene            complement(5025..8135)
                     /locus_tag="PK742_06035"
     CDS             complement(5025..8135)
                     /locus_tag="PK742_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HQU76183.1"
                     /translation="MAEKKYWKGLEELHPTPEFIELAQQEFPEELPVEVMGNHENNGA
                     SRRDFLKMLGFSVTAAAIAGCEMPVNKVAPYIFKPEDVTPGIATWYASTFMNGGDYCS
                     ILVKTREGRPIKVEGNNASMITRGGTSARVQASVLSLYDTNRLTGPKVRNDKGKHTGI
                     SWAEVDAAVTGKLNAGENTAVRILTGTVVSPSTKRVFARFTEQYPNTKVYQYDAVSYA
                     GMLAANETAFGVKALPHYHFEKARVVVGFECDFLGTWISPVEYTKGYSAGRKVGKGKK
                     EMSRHYQFESRMSLTGANADYRTALKPSELMTALVNLYDEVTGASLSGGKKIGDDKKA
                     KRIREAAKWLKDNAGSSLLVCGLNDMNAQLLVNAINNALGNYGRTLDMSMPSMLRQGT
                     DNDLKAFISDVSKGEADVVMIYGCNPVYDSPLGSSIAEALKKVDVCVSFADSMDETSA
                     VCHYICPDSHYLESWNDAEPVKGCYSLAQPAIRNLFDTRQAQDSLLAWCGASMSYHDF
                     MVETWVNGPLAGSANMTRAFNQALQTGLFQMPMEDMGDHEMMYDPQPVVMNGDVSSAA
                     AAVLSSGSKMGNGLEVTFYESVAIGDGRYANNPWLQEMPDPVSKITWDNYLNVSPEWA
                     EENDLMHGDVVTLKAAGQSVTVPVAIQPGQAKGSASIALGYGRSMIGKAGEGVGVNVW
                     PMVDASGDCLVYCASGATVEKTGKNIRFAQTQTHFNLNDGLAKRTIVKETTLAEYQAN
                     PLSGNMDRAEKLGHLDTFYPDFQAGRNGFDWGMSIDLNSCVGCGACTIACISENNVPI
                     VGRDEVLRAHEMHWIRIDRYYAGEDSENPEVVFQPMLCQHCENAPCENVCPVGATNHS
                     SEGFNQMAYNRCIGTRYCANNCPYKVRRFNWFDYGGNDTFGTMNDPHVNEEMGMLEDL
                     TRMVLNPDVTIRSRGVIEKCSFCVQRIQEAKLGAKKDGRQLRDGDVRTACQSACPADA
                     IVFGNMFDETSQVHQLNTSERAYGVIEENHWLPSVLYLTKVRNKDAADHTHEQELDIM
                     DLYNYQ"
     gene            complement(8178..>9513)
                     /locus_tag="PK742_06040"
     CDS             complement(8178..>9513)
                     /locus_tag="PK742_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HQU76184.1"
                     /translation="ETPDVDLQRFLRKPYLNLNTLVVLNSVYNKINRRNLLVSVLLLV
                     TILVPGRVHATAEEGKELFKANCAKCHMIDRKMTGPALKGVRDRWADSTHLYSWIRNS
                     QAFLATGDEYANNLYLEYNKSQMQAFPNLTDADISNILLYIDAETIRLTEAANVPAGG
                     GGTAVAAEPKDNTVLYIILIAVLLIISLILGRTIGYLGQLSREETGEPEPVRMPVLRN
                     PRLRPVLTLVGLFLFCWLCYAMYDSAAALGRQQGYAPEQPIQFSHEIHAGINQIECKY
                     CHTGAEKGKSAVIPSINVCMNCHYVINQMSPGTDGYDPATYTAEIQKIYEYAGFDPEN
                     LAYSNEPKPIKWTRIHNLPDHVYFNHSQHVAVAGLECQTCHGMVQEMKVVEQAENLGM
                     GWCVNCHRQTEVNFSNAFYADYRELKDKMDKGEIEAVHASDIGATECQRCHY"
BASE COUNT         2432 a         2379 c         2222 g         2480 t
ORIGIN      
        1 gccccatggt tgaccggcag gtgcagttca gacgggagaa aatcatcgta tccccaggtc
       61 agtgcttctg cattggccat atagtagtct tccgtatatt tcttagtgat gcggtctaag
      121 gtctggtttc tgcgcagaat atgatcatct atctcatcca tttcatcttc cagtgcatcc
      181 agttcttcct gcaactgatc tttcatatat ctgttggggc gtttggcgat cttgtcttcc
      241 aaagccgcaa taagaccggg taactcagac ttgcggtttt caagagcagc gatctctcct
      301 tccacaccca gttcagcatt acctttcagg gtattgatac ggtgttccac gtactcgcgg
      361 gttacaatac ccagcgggtt ttctgcacag atcagactat aatcacgagg acccagttta
      421 ccgtcggcac ccggatagcg actggtccag aaaaactgct gaccggtcat ttcaaagatc
      481 acggcatctt cttcaggcgg acccatgatg cgggtccatg cctgcaatcc aaaaaccacc
      541 agaccagtca atactatagt aggtacaacg gtccagaaaa attccagttt gttgctatgt
      601 gcaaagtgta ctgcttcgcg gccttttttg tatctgtaca tcagcacgaa ggtgaaaagc
      661 agcacattac acagcacaaa tacgatgctg gtaaccacaa gagtagcaat gaacatgttc
      721 tcaatgagtt cgccgtgttc tgaggctaca tctacatttg ttggtgcaaa gtgccggaag
      781 ctccagaaga acccgataac gcccaaagcc atgaagatga acaaagcaat tgcctgcatc
      841 cttgaggtct gctccagctg gctgtcatca tcaccacgca actggcttac cagatccagt
      901 gtgcgggtaa cctgaaacag tacgatcagg acgagcaata cagctatgaa tatcaaccaa
      961 ccagtcattg ttgttgctta tttaattata tatggtgttc aatactttct tggataaacg
     1021 gattgtgttg aggcacaagt gacacttttg acaatgcccg gaaagtagaa tacaggaata
     1081 cacctgcaaa tgccagggcc ataccgatct caaagtagcc aattggagca gcgccatgaa
     1141 cggtagatgg aatgatcatc aggtagaaat ctacccaatg gaacacgatc agcaatgatg
     1201 ctactacggt caacacagta aagtttcttt tggcatctct tctcatcaga acaaggaaag
     1261 gcaccaggaa gttgactgcc aggttcgctc cgaacagaaa attatattcc cttttccttt
     1321 ccagaaacca cgctgtttct tcaggaatat tgccatacca gatcagcatg aattgtgaga
     1381 accacagata ggtccagaat acactaaatc cgaagatgta cttacccaga tcatggaggt
     1441 gattctcatt ggtttgcggc atgtaaccat ttctcttcag ataaatgatc agcaatatta
     1501 ccacagacaa agaagccacc cacaaggaga tgaagttata ccagccaaac agggtggaat
     1561 accagtgtgg atcaagcgac atcacccagt cccaggccat catagaactg gaaactgccc
     1621 atacaaccag gaaaactgca gcccaggtac gagtcttttg aaaccacatt ttctctcctg
     1681 tttcttcttc ttttatggag aacctgcgca actgataccc aatgaagacc cacaatacca
     1741 gataaatgat cgtccgggtc ataaagaatg catcgttcag ataggctgac tttccatcca
     1801 gtatctcatc ataatatgga ttatctatca tacctgattc tccgccttca gaaccggcgc
     1861 tggccagaac cactgttcca ttactgagca tggagtgttc ttcaccgtgg tgttcgccag
     1921 attcgtggtc accttccatt tcatgcccgt gtgcatgttc tttttcatag gcctccagtt
     1981 cggccatggt gaccttttcc ttgatcagga aggtatcgct ccagtgatag aggttgttat
     2041 aattgccgat agtaccgcct gttacaacca ggatacagat agccccgatc cagagaaagc
     2101 tggtcatggc ttccggcacc ctcttaacca gcgcatgcca tccggcaaat gcgatctggt
     2161 gagccgccag gaaaaaaaca gccagggctg ataagccggt aaaaaatacc gcattcacca
     2221 acaggttcgc ccaggttttc tgtgcatctg cctgaccggt aaaaacacct gtcagcacag
     2281 ccatcatacc gatcacaaaa aggattatga agttcctttt atgtgtggaa gtgaaatcaa
     2341 attttgttgt actgctcatt gctatctgct gtttcaggga tttacatctg tttcggcaac
     2401 ctgtgtctga gccggctggt tagaagcaag ccaggcatcc tgcatagagt tgatataaag
     2461 aatgatctgc cagcgctctt tcttggtaag tgcataggca taactaccca tggcattctt
     2521 accatatgta atagagtgga acatcttacc atcaggcatg tcaatgtatc ctgcatcaaa
     2581 atatgatggc ggcactgctc catattttcc ggaagcaaca atcactcctt ttccatttcc
     2641 ctctgttcca tgacaaaccg cacacatatt gttgaaggca tgctgacctg ctgcaatatt
     2701 ctcaggagtt gcctgcagcg ggtttatcag ctcaaggccg gcacgttcat agtcttcatc
     2761 tgtcttagcg tattcgtagg gaaggcttat agatgccaga gcaggatcca tctggcctga
     2821 aatagcattt ccaggataag gaatggtacc atttaccgga agcattgcac ctacactgtc
     2881 aggcatcaga ggattcaaag cgtatggctc atacgctttg gaatacatca tatcccaggt
     2941 ataactatcg cccggataat ctcccgaggc cttcttacat gcctgcagtc cggtaagaac
     3001 agcggcggcc atcaatatga gaaaatagct tcttttcatc tctgatattt tcagtcgagg
     3061 tcttgttcat gaatttcaac cgcaccattg gcttccagtg ccttgcgaat ttctgctttc
     3121 ctggcatctg aagtgtctgc gtcaatttca aaactcatcg caaacatgtg gctggtgatc
     3181 ctttcatcca ggatccgatg tttaataccc ggagccagtc tgttcagata cagataggta
     3241 agcgtcatac caacagcagc gaacagcact gttaattcaa atgtgatcgg gatccaggcc
     3301 ggcaaagaga agtatggttt gccgccatag ttgatgggat agttcaccgt actgatccag
     3361 gtaatgaaac ccagggctgt cagtgtgccg gtagcaccaa acagaaaacc tgcactgtgc
     3421 aacttggtca tacgaagacc catggcttca tccagcccgt gtaccggaaa aggtgtcatc
     3481 acctccgtga tcttgaagcc ttctgcacga acacctttta cagcatccag cagtacatgc
     3541 tcatcatcaa acaatccgaa taatactttc tttcctgaca tgttattgcg gtttaatcag
     3601 ggtttcgtaa tgtccatgac ctgcgtcttc agtatgatgg gcatgggctg catgctcacc
     3661 gatatactga tcgccggccg atttcagaat actcttcact tcagccagtg cgatcacagg
     3721 tgccactttg gcgaaaatga gaaacagcgt catgaagaat ccgatggttc ccagatagat
     3781 gaatacttcc acataggtgg gtgtatagta agaccagctg gaaacgatat aatctctgtg
     3841 gagggaggtc acgatgatca cgaagcgctc aaaccacata ccgatgttaa tgacaatgga
     3901 cataaagaag gtgaagacca cactgcgacg cagtttgcgg aaccagaaca gctgcggaga
     3961 gatcacgtta caggtcatca taccccagta tgaccaggca tatggcccca tgcttcggtt
     4021 gatgaaagca tactgttcat actgattctg agaataccag gcggtaaaga gctctgtaat
     4081 gtaggctaca cccacaatag aacctgtcag aatgatgatc ttgttcatcg actcgatatg
     4141 cgacagggtg atatagtctt ccaggtgaaa gatcttgcgc actaccagca tcagggtcag
     4201 taccatagca aatcctgaga aaatggctcc ggctacgaag tagggcggga agatggtggt
     4261 atgccatccc ggaatgaccg atgtggcaaa gtcaaaggat acgatggtgt gtacagaaag
     4321 taccagtggt gtggacagac cggccagcac cagtgacaga ctttcaaaac gctgccattg
     4381 tttggcagtg ccggtccatc cgaaactgaa cacattgtag atctttttgc ggaatgctgt
     4441 tttggcacgg tcacgaacag ttgcaaggtc cggaatcaga cccacatacc agaacaggag
     4501 tgataccgtg aaataggtcg agatggcaaa tacgtcccac agcagtggtg agttgaagtt
     4561 ggaccaaacc ggaccgcggg tgttcggata agggaagatg aagaaggcca gccagatcct
     4621 acccatgtga atgagcggga aaagtccggc gcacatgacc gcaaagatgg tcatcgcctc
     4681 tgccgaacgg ttcacccctg tacgccattt ttgacggaag atcaggagga tggccgagat
     4741 cagcgtaccg gcgtgaccga taccgatcca ccaaacgaag ttggtgatat cccaggccca
     4801 gccgatggtc ttgttcagac cccagagtcc gataccgtag gtgatggtca gtccgatcat
     4861 cacaaatccg gtgatggcca gtaatgccgt acaagtaact gccagcatcc aggttttcgt
     4921 gggttttgcc tctaccggtc cgatgatgtc atcagacatc tgggcatagg tcttatgccc
     4981 ttgaatgagt ggttccctga ttggtgaaac gaacatctct ttgcttattg atagttatac
     5041 agatccatga tatcgagctc ctgttcatgt gtatgatcag cagcatcctt gttgcgtacc
     5101 ttggtcagat acagtaccga tggtaaccag tgattttctt cgatcacacc gtaggcacgc
     5161 tcgcttgtgt tgagctggtg tacctggctg gtttcatcga acatatttcc gaaaacgatc
     5221 gcatctgcag gacaagcgct ttggcaggct gtgcgaacgt caccatcacg caattgacgg
     5281 ccatccttct tagcacccag tttagcttcc tgaatacgtt gtacgcagaa ggagcatttt
     5341 tcgataaccc ccctggagcg aatggtcaca tcggggttca gcaccatgcg ggtcaggtct
     5401 tcgagcatgc ccatttcctc attgacatga ggatcgttca tggtaccaaa agtgtcgttt
     5461 ccaccgtaat cgaaccagtt gaagcgacgc accttatacg gacagttatt agcacagtag
     5521 cgggtgccga tacaacggtt gtaagccatc tggttgaatc cttcagagct gtggttggta
     5581 gcacctaccg gacaaacgtt ttcacaaggt gcattttcac agtgctgaca aagcatcggc
     5641 tggaagacca cttccgggtt ctctgaatct tcaccggcat aatagcggtc aatacgaatc
     5701 cagtgcattt catgtgcacg gagcacttca tcgcgaccca caattggcac gttgttctca
     5761 cttatacaag cgatggtaca tgcaccacat ccaacacagg agttcaggtc tatgctcatt
     5821 ccccagtcaa agccgttacg acctgcctgg aaatccggat aaaaggtatc aagatgaccg
     5881 agtttctctg ctctgtccat atttccggag agcgggttag cctggtattc agccagggtg
     5941 gtctctttta caatggtacg tttggcaagc ccgtcattga ggttgaaatg cgtctgtgtc
     6001 tgggcaaagc gtatgttttt gcctgtcttt tccacagttg caccggaagc gcagtacacc
     6061 agacaatcgc cagatgcatc caccatcggc cagacgttca caccaactcc ttcaccggcc
     6121 tttccgatca tgcttcttcc ataacccaat gctatggagg cgctgccttt tgcctgaccc
     6181 ggctggatgg caactggcac ggtaacagat tgacctgcag ctttaagtgt taccacatca
     6241 ccatgcataa ggtcattctc ttctgcccat tctggtgata cgttcaggta attatcccag
     6301 gtgatcttgg agaccggatc cggcatctcc tgtaaccatg ggttatttgc atatcgtcca
     6361 tcgccaatgg caacagattc atagaaagtc acttccaggc cgttgcccat tttagaaccg
     6421 gatgagagta ctgctgctgc agccgaagaa acatctccgt tcattactac cggttgagga
     6481 tcatacatca tttcatgatc acccatgtct tccattggca tctggaacaa acctgtttgc
     6541 aaggcctgat taaatgctct ggtcatattg gcacttcctg ccaggggacc attgacccag
     6601 gtttctacca taaaatcatg gtaagacata ctggcaccgc accaggcaag cagtgaatcc
     6661 tgtgcctgac gggtgtcgaa caggttcctg atagcaggct gggccagact gtaacagccc
     6721 ttcacaggtt ctgcatcgtt ccatgactcc aggtagtgac tgtcaggaca tatataatgg
     6781 caaacagcag aagtttcatc catactgtca gcaaaggaga cacaaacatc tacctttttg
     6841 agtgcttccg cgatgctgga acccagtggt gaatcgtaca ccggattaca accatagatc
     6901 attaccacat cagcttctcc tttagataca tcgctgatga aggccttcag atcattgtca
     6961 gtaccctggc gaagcatgga aggcatgctc atatccagcg tacgtccgta attacccaat
     7021 gcgttgttga tggcgttgac cagcaactgt gcattcatat cgttgaggcc gcaaaccagc
     7081 agggatgatc cagcgttgtc tttcagccat ttggctgctt cacgaatgcg tttggctttc
     7141 ttgtcatcgc cgatcttttt gccacctgaa agtgatgcac ctgttacttc atcatacagg
     7201 ttcaccaggg cagtcatcag ttcagatggt ttcagggcag tgcgatagtc agcattggca
     7261 ccagtcagac tcatacggga ttcgaactga tagtggcgtg acatttcctt tttcccttta
     7321 cctacttttc ttccggcgga ataccctttc gtatattcca caggtgagat ccaggtgccc
     7381 aggaaatcac attcgaaacc tacaacgacc ctggcctttt caaagtggta gtgcggcagt
     7441 gcttttacac caaaagcggt ttcattggct gccagcattc ctgcataaga cactgcatca
     7501 tactgataga ctttggtgtt agggtattgt tcagtaaagc gggcaaaaac tcttttggtt
     7561 gacgggctta caactgtacc ggtcaatatc ctgaccgcgg tattctcgcc ggcattcagc
     7621 ttaccagtta cggcagcatc tacttcagcc caggaaatgc cggtatgttt ccccttgtcg
     7681 ttcctcactt tagggccggt aagcctgttg gtatcataca agctcagcac agatgcctgc
     7741 acacgggctg aagtaccacc cctggtgatc atggaagcat tattgccttc taccttgatc
     7801 gggcgtcctt ctcgtgtttt aacaagaatg gaacaatagt ctcccccgtt catgaaggtg
     7861 ctggcatacc aggttgcgat acccggtgtt acatcttccg gtttgaagat ataaggtgct
     7921 accttattga caggcatttc acaaccggca atagcagcag cagttacgct gaatcccagc
     7981 attttcagga aatctctcct gctggcgccg ttgttttcat ggtttcccat cacttccacc
     8041 ggaagttctt cagggaattc ctgctgagcc agttcgatga actccggagt cggatgaagt
     8101 tcttccagtc ctttccagta ctttttttca gccatcgggt atctagatct ttatgtttaa
     8161 agttgggttc ggttcgatca atagtggcaa cgctgacatt cagtggcacc gatgtctgat
     8221 gcgtgcacgg cttctatttc tcctttgtcc attttatctt tcagttcgcg gtaatcagcg
     8281 tagaaagcat tactgaagtt gacttcggtc tgacggtgac aattcacaca ccatcccata
     8341 cccaggttct ctgcctgctc taccactttc atttcctgaa ccatgccatg acaggtctga
     8401 cattccaggc cggctactgc cacgtgctgg ctgtggttga aatacacatg atcaggcagg
     8461 ttgtggatcc tggtccattt aatgggctta ggttcattgc tgtatgccag attctccggg
     8521 tcaaaacctg cgtattcgta gatcttctgg atctctgctg tgtaggtagc aggatcgtag
     8581 ccatcggttc ccggcgacat ctggttgatc acatagtgac agttcataca gacgttgata
     8641 gatgggatca cggcagactt gcctttctca gcaccggtgt ggcaatactt acattctatc
     8701 tggttgatgc cggcgtggat ctcatgactg aactggatgg gttgttcagg agcgtatccc
     8761 tgctggcgac ccagggcagc agcgctgtca tacatggcat aacacaacca gcagaacagg
     8821 aacaaaccga ccagggtcaa caccgggcgc agtctaggat tcctgagcac aggcattctg
     8881 acaggctcag gctcaccggt ttcttcacgg gataactgcc caaggtagcc gatggtacgg
     8941 ccaagtatca gagagatgat gagcagtaca gctatcagaa tgatatacag aacagtgttg
     9001 tcttttggtt ctgcagctac cgcagtgcca ccgcctcctg caggtacatt ggcagcttca
     9061 gttaacctga tggtttctgc atcaatgtat aacaatatgt tgctgatgtc tgcgtctgtc
     9121 aggttgggga aggcctgcat ctgacttttg ttgtattcca gatacaggtt gttggcatat
     9181 tcatcgccgg tagcaagaaa ggcctgggag ttcctgatcc aggagtacag gtgtgtagag
     9241 tcagcccaac ggtcacgaac acccttcagg gcagggccgg tcattttccg gtctatcatg
     9301 tgacacttgg cgcaattcgc cttgaaaagt tcctttcctt cttctgcggt agcatgtaca
     9361 cggccaggta ccaaaatggt taccagcagc aatacagaaa cgagaaggtt acggcggttg
     9421 attttgttat agacgctgtt cagcacaacc agtgtattta ggtttaggta aggtttccgt
     9481 aaaaatcgtt gcaaatctac atcaggtgtt tca
//
LOCUS       DAOMAX010000084         9384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1016_length_9384_cov_4.367349, whole genome shotgun sequence.
ACCESSION   DAOMAX010000084 DAOMAX010000000
VERSION     DAOMAX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1016_length_9384_cov_4.367349"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PK742_06045"
     CDS             <1..200
                     /locus_tag="PK742_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopenicillin N synthase family oxygenase"
                     /protein_id="HQU76185.1"
                     /translation="HRVVNPPREMWHTSRFSIPFFLHPRSEMDLTCLDSCVNEENPLQ
                     YEPISAGGYLDERLREIGLKK"
     gene            197..727
                     /locus_tag="PK742_06050"
     CDS             197..727
                     /locus_tag="PK742_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76186.1"
                     /translation="MILDQPSYERFVAGKTRLIPDNVKVVVALFAGGAAWNTGLSYLT
                     IVLVSLDLLMMTLKSGVETQLSQRRYRDYVDVLFMRIGKWKQLAPIEAILVSRTGERS
                     WAVFGALTFNVKSKSARYMVDIITTGDRDVIILNTMDQEAAYELARSLAIQEDVKLYE
                     QASGGHATEISPYHND"
     gene            complement(805..2823)
                     /locus_tag="PK742_06055"
     CDS             complement(805..2823)
                     /locus_tag="PK742_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76187.1"
                     /translation="MKKLYLLLALGAPLAVWAQCNTGNATDCDCLDGSTDCDLLPDMR
                     ASYILLADPDYQPEYVGELGVSVSTPNTGHGPLRVFATDYYICDGDTVYEPGGLDVCS
                     DGTAPRQIIKQRIYHKNGTEMSYWERNAGTMTYHPGHGHFHVDDWGIYTLREEVPGED
                     PLDWPIIGSGSKLGFCLMDYGSCGSGYYYAYCENDLGEVLTSDAPNYGLGGGGYSCGL
                     TNQGISAGYLDIYHYWLEGMSIFLPDNICNGDYKIVVQVDPYNYFLEENDDNNVMVAD
                     VTLTMQPESDEGIVTINGSTFICEGETVELSAPNVGTSYMWSNGATTSTIEVAEPGEY
                     YCTVERSCGMLYTDTISVTIADIAEPVVDDPGVTCEGASATLNASGDDVTWYDADGFV
                     LGSGNTYNTLPLYEDTYLYAANTAYYPVDGYAEPHEHTGGSDYNGDTYNGYLIFDASQ
                     EFTLKSVKVYTDTPGERTLELRSAAGAILETATVDVPAGESRVDLNWVIETGNNYQLG
                     TNEAQNLDALGYQSPRFKRNSGDVYFPYNVGGVASIHTSSAAGYYYYFYDWEYDAGKV
                     CTSLQTEVFLKVEVCDGISEIAGIKDLDIYPNPNSGSFSVSADFANTTDLSIRVSNLT
                     GQVVFENHPGQVVGAVTIPVTLTNQPAGVYTVSLISEGKAVNRQVVVE"
     gene            complement(2930..5248)
                     /locus_tag="PK742_06060"
     CDS             complement(2930..5248)
                     /locus_tag="PK742_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU76188.1"
                     /translation="MKENDYDHFLTHRSYGIPDPEPVQHPEAGMLPWNAPCTDCYEDL
                     DRRTPNTRTFIRQNGSWIREQSYGAMHYTDDAGFLRTTDPHLYPSEISGIYKAPAQLL
                     PTFLNTIEGYAALQLANGYIWQHDHNLRYNGNLLQYADVHAGMDGAWLHDAWLLTDLE
                     MVFGKGSIKTNYILNSIDAVQADLPVQEFSETFQLPEGMRIVPETENGYYTNAGWWMG
                     SVAVEQYGVKLATIGAPWVIDQQRSKFHDEAQLQASGYLIEQDGNSWTIRLLVKTDWL
                     LAPERQYPVIIDPLLTGEATYTASDIGFEFNTVCWDDADYCGYSMDITVPGTTTLTAA
                     YFDGTYYSQNFGCFFTTDCLMSEAAFRILGPCDDSPSPTSFWTCIPPVGDSSGTCWGI
                     DLDMFNTIACIPPQCEDYVFNFEMRTYHCSCTKPPCDITCHYMPSDSWIITIEGQTVV
                     ETPVISNTHPDFIICAGDSIDLFTGGYWGVPPYYYEWLPDGTISDTIWVAPDTDTWYT
                     AIIHDLCEVQDTLQQLVTVLPAPSLSPGPFEGCYEVEANAGSGYASYVWSDGQTGQFA
                     TFDSSGTYYVTVTDGNGCVGISDPVEVIVHTYPNINAIPDTVFVSDGELAEMSVTTTS
                     TGDVTFNWIPAENVTCTDCADTYGIVYTALGYFYVTGEEFGCVSPPDTVVVINTQTDL
                     IVPNAFTPNADGLNDVFKPYSELIYPKYELYIYNRWGEEIFFSDDMLFGWDGTRNAME
                     QEVGTYIWVITYQRFNDQGKVYTLKGTVTLLR"
     gene            complement(5398..6096)
                     /locus_tag="PK742_06065"
     CDS             complement(5398..6096)
                     /locus_tag="PK742_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76189.1"
                     /translation="MKKSIFFYLLFICPLFVFAQDDDAGQVIYLGTGTVLSGFGYSYY
                     TPSVFYERSLAGRQSLFAGSHLALGSGSGMRLQLAVRGYSNENTWSSFYVSPGFSAGV
                     HMMDIRYQAPSQDDVQWVDVYEPSTFVFLPFAFASMLIEDNEIYDQQLRYMAGVQLAV
                     GYRKALGARQRWIADAQIQAGYYAPLNDPEPTVTYMDQVLTLSPNGTSNPSSWSKSFL
                     GKPLRGQVLVGYRF"
     gene            complement(6147..9071)
                     /locus_tag="PK742_06070"
     CDS             complement(6147..9071)
                     /locus_tag="PK742_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU76190.1"
                     /translation="MNPKYLWTLILLLATASVSSQTFQLFAEDFDDDYAGSFLLNSAG
                     PGDSIGNNQWIINDSYTGGFGYPNTPSQLMVESGTIGGAPYSTYLHVHDVEAAPAVIN
                     ASYDPGSASDHFAEMAYGFCTLGLVDIEFTFFWIGEGSATAYGQVYYSADGGPWTEVG
                     EPLYNEQSLWKYEVITSTDFEDVADLRFGFRWLNNVSGDDPSVSFAIDDIIAVGTYDA
                     LASPVDINITFLFPDPVCALSTLVFGWELSAPLCDGTYQVQLSNASGSFASPTDLGVF
                     DIASADTSGAVAVVIPGTTPEGDCYKVRVVRLSPLPYIVGEASVCFEVQDCPNTITTL
                     PPVVTYDTNAVCVNSVIDVPFFSTGVYLPGNVYTAQLSDSTGSFDSPYTIGTFTSSAT
                     YDPALGSMPGMVSGIVPVVPPGCSYFIRVVSSAPEVTGSVYGPLCIQECDIETNDITD
                     IYVCISEEIGDTLTITYDTNVFDSIATYCPDNTFCVEILDAMFFNQASLCDLGITVDT
                     ESGTIELMIPGYTDLLALGLDAGVWYMRVIASCADPSENSLGTLIHLTIGAPADDPPV
                     LIPEDTLFCEGAVIAATVVPYNPNSVYQFQFGAGTPFTWPFNPILINFAGVTGDVSLK
                     VREINYGCPGPWSDSIVFHVIDVPVVAISGPAKACTGDTVTYSVPYFLSTYYDWSITG
                     GTVVDTANNEISVVWDAEGYYTLTIFALNECGSGTGNKTIHIIPTIPIGDVPDQLICE
                     GQTVNLNANAAGIEGYAWYEYGDTTLVYDDFYFSVTPDSTITYLLVGTDEEGCNSSDT
                     VMIAVEYPTNEFDTAIFCIGTQALLDAGHPGSNYSWSTGATTQQITAGMTGIYNVTID
                     PADQVCNIFKEFRVEMVTDTCDPIVEIPNAFSPNNDGVNDFFSLYGAAVTDLDLLIYN
                     RWGELVYETHDIGIVNNMQAGWDGTFRGEPQEMGAYAYFLKATGGSGNSVVLKGSVTL
                     IR"
BASE COUNT         2392 a         2356 c         2299 g         2337 t
ORIGIN      
        1 cccatcgtgt ggtgaatcca cccagagaga tgtggcatac cagccggttc agcattcctt
       61 tcttcctgca cccgagatca gagatggatc tgacctgcct ggacagttgc gtcaatgaag
      121 agaatccact gcaatacgag cctatttctg caggaggcta cctggatgaa agactgagag
      181 agatcggttt gaaaaaatga tcctggacca accctcttat gagcgatttg ttgcaggcaa
      241 gacccggctg attccggata atgtaaaggt cgttgtagcg ttgtttgcag gcggggctgc
      301 ctggaatacc ggcttgtctt atctgaccat cgtgttggtc tctctggatc tgttgatgat
      361 gaccttaaag tctggtgtag aaacacaatt aagtcaacga cgttaccgcg attatgtcga
      421 tgtgctgttc atgagaattg gaaaatggaa acagctggct cctatagaag ctattctggt
      481 atcgagaaca ggagaaagat cctgggctgt tttcggagca ttgaccttta atgtgaaaag
      541 caagagcgca aggtatatgg tggacatcat caccaccggc gatcgcgatg tcatcatact
      601 caataccatg gatcaggagg cagcatatga acttgcccga tcgctggcca tacaggaaga
      661 tgtgaaactg tatgagcagg cttccggcgg gcatgccaca gaaatttcac cgtaccacaa
      721 tgactaacct gtaagcattc gtaataccaa ataaaaagcc cggttgtaag tctttacacc
      781 gggctttttg tttttattga tatgttattc cactaccacc tgtcggttca ccgctttgcc
      841 ttcgctgatg agcgacacgg tgtacacgcc cgcaggctgg ttggtaagag ttaccggaat
      901 ggtgaccgca cccaccacct gaccaggatg gttttcaaac actacctgtc cggtcaggtt
      961 gctgacacgg atgctgagat cagtggtgtt ggcaaagtct gcactcacgc tgaagctgcc
     1021 actgtttggg ttgggataga tgtccaggtc tttgatacct gcgatctctg agatgccatc
     1081 acaaacttcc actttgagaa atacctctgt ttgcaggctg gtgcatactt tacctgcatc
     1141 atattcccag tcatagaaat aataataata tcctgctgca ctgctggtat gaatgctggc
     1201 cacacctcca acgttatagg ggaaatatac atcacctgag tttcgtttga accgtggact
     1261 ctgataaccc agcgcatcca gattttgtgc ttcattggta cccagctggt agttgttgcc
     1321 ggtttcgatg acccagttca gatccacccg gctttcgcca gcaggaacat ctaccgttgc
     1381 tgtttccaga atggctccgg cagcgctgcg cagttcaagt gttctttcgc ccggagtatc
     1441 agtataaacc tttacacttt tcagggtgaa ttcctggctg gcatcaaaga tcagatatcc
     1501 gttgtaggta tcgccattgt aatctgaccc tccggtatgt tcatgcggct ctgcgtaacc
     1561 gtcaaccgga tagtaggcgg tattggctgc atagaggtac gtgtcttcat agagtggcag
     1621 cgtgttatag gtgtttccgg aaccaagcac aaaaccatcg gcatcatacc aggtcacgtc
     1681 atcgccggat gcattcaggg ttgctgatgc tccttcgcag gtcacaccag gatcatctac
     1741 taccggttct gcaatatctg caatggtcac tgagatggta tctgtgtaca gcattccgca
     1801 gctgcgttct acagtacaat aatattcgcc cggctcagcc acttcgatgg tactggtggt
     1861 agcaccattg gaccacatat aggaggtacc tacattggga gcactgagtt ctactgtttc
     1921 accttcgcaa atgaaagtgc tgccattaat ggtcacgatg ccttcatcac tttccggttg
     1981 catggtcagg gtcacgtcag ccaccataac gttgttgtca tcgttctctt ccagaaagta
     2041 gttatacgga tccacctgca ccacgatctt gtaatctccg ttacagatat tgtctggcag
     2101 aaagatgctc ataccttcca gccagtagtg atagatatcg aggtagccgg cgctgatgcc
     2161 ctggttggtg agaccgcagg aataaccgcc accgccaagt ccatagttgg gtgcgtcgct
     2221 ggtgagtact tcgccaaggt cattctcgca gtaggcatag taatatccgc tgccgcagct
     2281 gccgtaatcc atcaggcaga aacccagttt ggatccggaa ccgatgatgg gccagtcaag
     2341 cggatcttct ccgggcactt cttcccgcag ggtatagatt ccccagtcgt ccacatgaaa
     2401 atgaccgtgg ccggggtggt aggtcatggt gccggcgttg cgttcccagt aactcatttc
     2461 tgtaccgttc ttgtggtaga tgcgttgttt gatgatctgt ctgggtgctg ttccatcaga
     2521 gcaaacatcc agaccgccgg gttcatacac cgtatcgcca tcgcagatgt agtagtctgt
     2581 ggcaaacacc ctgagcggac cgtggcccgt gttcggggtg gacacagaca ctcctagttc
     2641 acctacatat tccggttgat aatcaggatc ggccagaagt atgtaggatg cccgcatatc
     2701 aggcagcagg tcacagtcag tgctgccatc cagacaatca cagtctgtgg cattgcctgt
     2761 gttacattga gcccagacag ctagaggtgc gcccagggcg agaagcaggt acagtttctt
     2821 catacggttg ttcgtaattg agtctataaa aataaggttt tacctgatta gacaactaaa
     2881 ataacctttt gtcatccttg cttttacagt aaaatgttac aaagtggctt cagcgcagca
     2941 gggtgaccgt gcctttcagg gtatagacct tcccctggtc attgaagcgc tggtaggtaa
     3001 tgacccagat ataagtgccc acttcctgtt ccatggcatt tctggtaccg tcccagccaa
     3061 acagcatatc atctgaaaaa aagatctctt ccccccagcg gttatatatg tatagttcat
     3121 atttcggata gatgagttcg ctatagggtt tgaacacatc attcagcccg tcagcatttg
     3181 gggtgaaggc attaggtacg atgaggtcgg tctgggtatt gatgaccacc actgtgtctg
     3241 gtggcgacac acagccgaat tcttctccgg tcacataaaa atatcccagg gcagtataga
     3301 caatgccgta ggtatcagca caatctgtgc aggtcacatt ttctgcaggg atccagttga
     3361 atgtgacatc accggtgctg gtggtggtca cactcatctc tgccagttca ccatcgctga
     3421 caaaaaccgt atcaggtatc gcattgatgt ttggataggt gtgcacgatc acctctacag
     3481 gatcggaaat acccacacaa ccattgccat cggtcacggt tacataatag gtgccactgc
     3541 tgtcaaaggt ggcgaactgt ccggtctgtc cgtcactcca cacataagat gcataaccgc
     3601 ttccggcatt ggcttctact tcataacaac cttcaaacgg accgggcgaa agggatggag
     3661 ccggcagcac cgtgaccagc tgctgtaagg tatcctgtac ctcacacagg tcatgaatga
     3721 tggctgtgta ccaggtatcg gtatcaggag ctacccagat ggtatctgat atggtaccat
     3781 ccggcaacca ttcgtagtaa tagggaggta cgccccagta tccgccggtg aacaggtcta
     3841 tactatcgcc ggcacaaatg atgaaatccg gatgggtatt gctgatcacc ggtgtttcca
     3901 ctaccgtctg tccttcgatg gtgatgatcc agctgtctga tggcatgtag tgacaggtga
     3961 tgtcgcaggg gggcttggta caggaacaat gataggtgcg catctcaaaa ttaaagacgt
     4021 agtcttcgca ttgcggaggg atgcaggcga tggtattgaa catgtccaga tcaatacccc
     4081 agcaggttcc tgaactatcg cccaccgggg gaatgcaggt ccagaaactc gtgggtgacg
     4141 gcgaatcatc gcaggggccg agaatgcgga atgctgcttc agacatcaga caatctgtgg
     4201 tgaaaaagca gccgaagttt tgcgaataat atgtaccgtc aaaatacgca gcggtcaagg
     4261 tggtggttcc gggtacggta atgtccatgc tgtacccgca ataatcggca tcatcccagc
     4321 acaccgtatt gaattcaaag cctatatcac tggctgtgta ggtagcttca ccggtgagca
     4381 agggatcaat gatgaccggg tactgccgtt caggtgccag caaccagtcg gtctttacca
     4441 gcagccgtat ggtccaggaa tttccgtcct gttcgatcag atatccggag gcctgcaact
     4501 gcgcctcatc gtgaaacttg ctgcgctgct gatcaatgac ccagggtgcg ccgatggttg
     4561 ccagcttcac gccatattgc tccactgcca cagagcccat ccaccagccg gcgtttgtgt
     4621 aataaccgtt ttctgtttca ggaacaatgc gcatgccttc cggcagctga aaggtctctg
     4681 aaaattcctg aacaggaaga tctgcctgca cagcgtctat actgttcagg atataattcg
     4741 ttttgatgct tcctttgccg aacaccattt ccaaatctgt aaggagccag gcatcatgca
     4801 gccaggctcc gtccatacct gcatgcacat ctgcatactg caggagattt ccgttgtaac
     4861 gcaggttgtg gtcatgctgc cagatgtaac cgttggccag ttgcagggct gcatatcctt
     4921 caatggtatt cagaaaggtt ggcaacagct gagcaggtgc cttgtagata ccggatattt
     4981 ccgagggata cagatgcggg tctgttgttc gcaaaaaacc ggcatcatca gtatagtgca
     5041 tggcaccgta actctgctct ctgatccagc tgccgttctg cctgatgaag gtccgggtat
     5101 taggtgtgcg ccgatccagg tcttcatagc aatcagtaca gggtgcattc cagggcaaca
     5161 tgccggcttc cgggtgctgc acaggctccg gatcgggtat tccgtaagat ctgtgggtta
     5221 aaaaatgatc ataatcgttt tctttcacct gcatctgctg ggcagcaagt ggcacagacc
     5281 accacaggaa aacaagaagt ggagcccgga ataaacaaga cgaaaatgtt ggttttggca
     5341 agcacattta cccctactaa aataggtgat ttacaccact tgcccgtcat tgtttcatca
     5401 gaagcgataa ccaaccagca cttgcccacg caatggctta cccagaaaac ttttgctcca
     5461 tgatgatggg ttggaagttc cgttgggcga caaggtaagc acctgatcca tataggtaac
     5521 cgttggctcc gggtcattaa gaggtgcata gtatcccgcc tgtatctggg catcagctat
     5581 ccagcgttgt ctggctccca gggctttcct gtagccaacc gccagttgca caccggccat
     5641 atatcgcagc tgctgatcat agatctcatt atcttcaata agcatggaag caaacgcaaa
     5701 cggcaggaac acaaaagtag atggttcata cacatccacc cactgtacat catcctgaga
     5761 gggtgcctga tagcgtatat ccatcatatg tacaccagct gaaaatcccg gcgatacata
     5821 aaacgaactc caggtgttct catttgaata accccgaaca gcaagctgca ggcgcatacc
     5881 gctaccggaa ccgagagcca ggtggcttcc agcaaaaagt gattgcctgc cggctaatga
     5941 tctttcatag aatacggatg gggtgtagta gctgtaacca aatccggaaa gcaccgtacc
     6001 agttcccaga tagatgacct gaccggcatc atcatcctgt gcaaaaacga aaagagggca
     6061 aatgaacagc agataaaaaa agatactttt tttcatagca atgagggtta tgccacaaac
     6121 ataaacacaa atatgataaa agcaaactat ctgatcaaag ttacactgcc cttcagaact
     6181 acgctgttac cactgccacc cgttgccttc agaaaatagg cataggctcc catctcctgg
     6241 ggttcgccgc ggaaagtgcc gtcccatccg gcctgcatat tgttgactat acctatatca
     6301 tgcgtttcat ataccaattc accccacctg ttgtagataa gcaggtcaag atcagtaacc
     6361 gctgctccgt ataaactgaa gaaatcattg accccgtcat tattgggaga gaatgcatta
     6421 ggtatctcca cgatcggatc gcaggtatca gttaccattt ccacccgaaa ctccttgaag
     6481 atattgcaga cctgatctgc aggatctatg gtcacattgt agatgccggt catgcctgct
     6541 gtgatctgct gggtagtagc gccggtactc cagctgtagt ttgaaccggg gtgacccgca
     6601 tcaagcaatg cctgcgtacc gatacagaag atggccgtat caaattcgtt ggttggatac
     6661 tctactgcta tcatcacggt atcagagctg ttgcaacctt cttcgtctgt acctaccagt
     6721 aaataagtga tggttgaatc aggcgttaca ctgaaataga aatcgtcgta caccagggtg
     6781 gtatcgccgt attcatacca ggcatagccc tcaatgcctg cagcgttggc attgagattc
     6841 acggtctgcc cttcgcagat cagctgatca ggtacatctc ctatcggaat agtgggtatg
     6901 atatgaatgg tcttgttgcc tgtgccactg ccgcattcat tcagtgcaaa gatggtcagg
     6961 gtataataac cttccgcatc ccagaccaca gagatctcat tgtttgctgt atctacaact
     7021 gttcctcctg taatggacca gtcatagtaa gtgcttaaga aatacggcac actgtaggtg
     7081 acggtgtcgc cggtacaggc tttggctggc ccgctgatgg ctaccaccgg cacatcaatg
     7141 acatgaaata caatgctgtc gctccagggc cccggacagc cgtagttgat ctcgcggact
     7201 tttaacgaaa catcgccggt tacccctgca aagttgatga gtattgggtt gaacggccag
     7261 gtaaatggtg tacctgctcc aaactgaaac tgatatacag aattgggatt gtatggcacc
     7321 accgtggctg caatgaccgc accttcgcag aagagggtgt cttcaggtat cagcaccgga
     7381 gggtcatctg ccggtgcccc gatggtgaga tggatcaggg tacccaggct gttctctgaa
     7441 ggatctgcac agctggcaat gacccgcata taccagacac ccgcatccag ccccagggcc
     7501 agcagatctg tataaccggg tatcatcagt tctatggtgc ctgattcggt atcaacagtg
     7561 atacccagat cacaaagact ggcctggttg aagaacatgg cgtccaggat ctccacacag
     7621 aaagtattgt cagggcaata agtggctatg ctgtcaaata cattggtatc ataagtgatg
     7681 gtgagcgtat cgccgatctc ttctgatata cacacataaa tatctgtgat atcatttgtt
     7741 tctatgtcgc attcctgaat acacaacggc ccatagaccg atcctgtcac ttccggtgcg
     7801 ctgctcacca ccctgatgaa atagctgcat cctggtggaa ctaccggtac tattcctgaa
     7861 accattcccg gcatagaacc cagtgccggg tcataggtgg cgctgctggt aaaagtgcct
     7921 atggtataag ggctgtcaaa gctgcctgta gaatcactta gctgagcggt atacacattg
     7981 cccggcaaat acactcctgt gctgaagaac ggaacatcaa ttacagaatt tacgcagact
     8041 gcattggtat cataagtgac cacgggtggc aaggtggtga tggtattggg gcagtcctgc
     8101 acttcaaagc aaaccgatgc ctctcctaca atatagggta atggactcag ccgcaccacg
     8161 cgtaccttgt aacaatcgcc ttcgggtgtg gtgccgggta tgacaacagc tactgcacca
     8221 ctggtatctg ccgaggcaat atcaaaaact ccaagatctg tgggcgaagc aaaacttccg
     8281 ctggcattgc tgagctgtac ctgataggtg ccatcgcata agggggcaga caattcccag
     8341 ccgaatacca gggtactcag ggcacaaaca ggatcaggaa acaggaaagt gatattgata
     8401 tccaccggac tggccagggc atcataggta cctactgcta tgatatcgtc tatggcaaaa
     8461 gaaacggagg gatcgtcgcc ggatacattg ttcaaccagc ggaatccgaa acgcagatcg
     8521 gctacatctt caaaatcagt tgaggtaatg acctcatatt tccagagtga ctgttcgttg
     8581 tataatggct ctcccacttc tgtccacggg ccgccatcag cactataata tacctgacca
     8641 taagctgtgg cacttccttc cccgatccag aagaacgtga actcaatgtc caccaacccc
     8701 agggtacaga acccataggc catctctgca aaatggtcgg atgcacttcc agggtcatag
     8761 ctcgcattga tcaccgcagg tgctgcttcc acatcatgca catgaagata ggtggaatag
     8821 ggggctccgc cgatggtgcc gctttccacc atcaactgcg atggagtatt gggataacca
     8881 aatcctccgg tgtaactgtc attgatgatc cactggttat tgccgatgga atcgcccggg
     8941 ccggcactgt taagcagaaa cgagcctgca tagtcatcgt cgaaatcttc ggcgaacaac
     9001 tggaatgttt gcgaactgac agatgcagta gccagcagca ggatcagggt ccataggtac
     9061 tttgggttca taggcctcag aataccaagt taaggaatat gagattattc ttaaaaacag
     9121 gcttcggcac ccccactttc aacttccatc ttcataccat ccacctttct taatctagtt
     9181 caatgcagcc gctgcatcac cattctacct ttacatcgta ttcatgcatg aactatgtaa
     9241 ttgtgctaac gtttcatgaa aactgacttt atggaaacag caagcggaca tattattctg
     9301 catcccgcca gagagagagg acatgcccaa cacggctggc tggattctta tcatagtttc
     9361 agttttgcag gttactatga tcct
//
LOCUS       DAOMAX010000085         9378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1019_length_9378_cov_3.594122, whole genome shotgun sequence.
ACCESSION   DAOMAX010000085 DAOMAX010000000
VERSION     DAOMAX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1019_length_9378_cov_3.594122"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK742_06075"
     CDS             <1..235
                     /locus_tag="PK742_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HQU76191.1"
                     /translation="AQPRISSFPGCHGVELLQDILHPHIFFTFSVWDDHEALERYRAS
                     ELFNTTWAKTKVLFNDKPEAWSVVGVGSGKGEK"
     gene            complement(403..924)
                     /locus_tag="PK742_06080"
     CDS             complement(403..924)
                     /locus_tag="PK742_06080"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQU76192.1"
                     /translation="MANWKQPEEGTFIPYQKTYFDLVPAGDLVDLITNQFNDTETLLL
                     RLSDDQAGHAYATGKWTIKEVINHLVDCERIMCYRALCIARGEQTSLPGFEQDDYVTA
                     SGANERSMKDLVNEFRTVRQASISLLRTFDDAALSRLGTANHHTVSVNALCHVIAGHE
                     LHHMKIIRERYLL"
     gene            complement(991..7284)
                     /locus_tag="PK742_06085"
     CDS             complement(991..7284)
                     /locus_tag="PK742_06085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU76193.1"
                     /translation="MKNLQKILLLCMMILFVSSGLMAQITGDQDVCIDETETYYSSLS
                     GTLTWTITPVSPATFPSGNSLFLTWNVPPGTYTLIADNGAASENLTVQVYATPAPVID
                     YLENDCNTHEGIEGAGAGVTACDTFCINELVEYAVPDDPLNTYLWTISGGTIVGPDNQ
                     STVYVIWDVAMTGGDFPCGRLTVTETNPAGCSGTDEVFGVSSYPNGTITVSPGISTIC
                     LNQSVAFSLSVTGGPFITYSWDFGDGTIVVNSGASPETHTYITSGTYQVVVTASTECC
                     STSDTVEVIVDNTEGPNIFCISAVCANTVGAEYCTDAVCSTYDWDVDGGVITSAPNQP
                     CITVDWGAGPVGTISLLVGGCSPGLCADTVFATVPIMPDGTFDITGPVVVCQGVTEPY
                     SAPYVPGASYTWTLIPPSGIPVTLPYGMPPYIQDITFSAAGTYQLICSMEHDLLDCVG
                     ADTLIIQTAPLLTLSGPASACDGSTVFFSSNYPADWEYNLPITPFGTATTSASTTFPG
                     PGVYHIIASSASVCNSPDSISIVINPLPATPVISGPSVVCPGGTYTYTVSGYSPTASI
                     VWTFTDVSGSIFGYDNSFDLTLPVAFTTGTLSVMVTENGCSNSASITLDAPGTPAPVI
                     TTSAANVCPDQTVTYSATLSYPGLSDVEWTISPPSAGTITPGLLPGTVDILWHGNASN
                     TTQTVTLTVTETTCLTSTGTDVITFDIYPRPVIVATGGAFCPGTSFTLTATGGNSYQW
                     FDENSVPVSATVTQQGSYYVVGTDGNGCTNIDYTDVFEYPEPEAFISTPDNVICNDDG
                     TAMTNSIDLYALDNGYTFMWSTGGTTNPITISNLGTYSVTVTDMNGCTNTDTYDVFCP
                     VPVEDTCDTAITPLCACDLFPPTFVQSSPDCNEFTFSSNSTCVGGVFNWTFGDGFSGT
                     GTSVVHSYNQAGYYHVYYSYYDPTCCPPLPEDTVVAVPVAADFAWEVVCNTLTVVDNA
                     SFLPPYSIVTWEWDWGDGSPLTYGSAPGVHTYAVPNTYPVTLTVYTAAGCEASYTQNI
                     TIGGPAVSTTISPSACNGPVAFAATVLGGNVIDWQWDFGDGFTSGQQNTEHTYDTPNT
                     YTWTLTATDAAGCQTTATGNITVVAPPASFALLYASPSCGPTTLSVPGAVIYTSYQWF
                     NNGTAIPGATGSTYTATLSGNYSVEVVDVDGCIINSLPAMITIYPQPVITFTFPPLLC
                     SDDDIIIQSNTYGLYTWDWKVDGIPYFAGPTLTIPGGTLGAGPHTVELTVSTTTGPTP
                     PCSDYQSVVIMVEQSPTVFVTSSDPGGVCSGTPVTLTANSATAVMWDWSNGDMTSTTI
                     VNAAGNYSVTVTDAVGCTASASAYANIFPLPDLSMLPIGCDSSCIYPTPEIIHGPPGM
                     LSYQWYIDNTPAGTLQDLELNTSNLPTFGVPYQIKLVATSIYGCVDSSEFQYTPLECE
                     VECFTIEDTIWCNDDGTYTMEIQVTNLGAATSTYLWLHDVNMPFTLTPSFVVPLTLAT
                     GATSAPISINVSYPIGSPLPAELCFLASLYNADTCCHDTTMYCIPLPPCGPCDNLVVT
                     ADTSAGCCATVNLTNGYNGSFFSGIEVAPLSPGVSIASLMVGPSYTGTWSAYGTASAM
                     YVQPTGGFVPMGSIPGLFDVCLNVTVVAPPVQLVAVNWLYTDADGNQVVACSDTLEFT
                     CPTPNVNPCGEISGDITCLGDGTYAYNYTFTNNNPNDVYMLVFTSVTPFVNITPNPVI
                     LPSAVAWGGSYTGTVIIDPLGLPAGTNICFDLTLADYDGWCCHEMDSICIPIPECDTC
                     QCNKWENFIANVDQPGALPDYSLIASCGAVYNSIPAGTVIDFISGGYSCLALDGTSCD
                     ADHSWTLTGPGTAIDGSGYPSFSLLNPGLYTLDVVGYCQGNSCDTCTMYFEVVTADCT
                     CGEWGLFTVSTPFKTWINRPCGSKFTVNAFDAISLNGVYNCSGACDAVYQWTIRQNGV
                     PYASGFGMPVNFTPTAAGNYQVQMTAICGGVKCDKCTMTFRVKDADPPLRIAEEDEQI
                     LRIQPNPASNEVTVRIDSPEEESGHLQVLNELGVPLRLEEMKLEKGSNTLLLDIADYP
                     AGIYYIQYVGNGRVLNGQLIIVH"
     gene            7424..8083
                     /locus_tag="PK742_06090"
     CDS             7424..8083
                     /locus_tag="PK742_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQU76194.1"
                     /translation="MTTNLSAQSHLEIPYIEVTGTAKLEVVPDEFYVNITLRERYDGR
                     DKITIDKQEEDLRRAFIAIGIPDTQFYLADVDADYMKVRWNTKDVITQKNYTLKVTTP
                     TLLSKAFQAMDEKEIKDAYLSRVDHSQMDSLRREVRMEAVRAAKDKATYLLAAIDTDL
                     GAPLIVREQEMYYGNRNMEMYNVRMDDMDKASEPLPELQFQKIQLEQSVFVRFAIAAA
                     Q"
     gene            8129..9373
                     /locus_tag="PK742_06095"
     CDS             8129..9373
                     /locus_tag="PK742_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQU76195.1"
                     /translation="MDLLSILIRLLSTGLAFFLPDLLLYGAVKKAWNSRGWKIYLISN
                     LVLFGIVFLSSFLLRESTLILQRIQLYFFGLFVAILFAKVFTGLLLLVELFIRFIYSL
                     FTKKKRKSEDAEAKTLISRRQFIAKGAILTGLLPMTGIIYGMAKGRFRFTLHEVDIAI
                     SNLPPAFDGFTITQLSDLHIGSFDHSTMHELADMVQEVNALGSDAIFFTGDIVNSRAD
                     EMDGWYELFRTFHAETKLSILGNHDYGDYVNWPDEAAKERNLDQVKAIHGKLGFQLLL
                     NERTVIEKDGQQLHIIGIENWGKGDFPKFGDLGKAAAGLTADDCMILLSHDPSFWDAH
                     VLDSPFQPELTLSGHTHGFQMGIETPAFRFSPSQWVYRQWAGLYAEGRQQIYVNRGLG
                     TVGYPGRLGIWPEITKIRLIRE"
BASE COUNT         2465 a         2269 c         2451 g         2193 t
ORIGIN      
        1 agctcagccg cgaatcagta gttttcccgg atgccatggc gtagaattac ttcaggacat
       61 cctccatccg catatcttct tcactttttc tgtatgggat gaccatgaag cactggaaag
      121 gtaccgtgct tctgagctct ttaacaccac ctgggctaag accaaagtat tgttcaacga
      181 caaacccgaa gcatggagtg tggtgggtgt gggaagtgga aagggtgaaa agtgaggagt
      241 gaggagtgga aagagtgagg agtgaggagt gaggagtgag gagtgaggaa tggaaagagt
      301 gaaaagtgag aagtgaaatg tcgctatacc cgatacctga tacctgatac ctgatacccg
      361 atacctgata cccgatacct gatacctgat acccgatact aactacagca agtaccgctc
      421 ccggatgatc ttcatgtgat gcagctcatg tccggcgatg acatggcaga gagcgttaac
      481 agaaaccgta tggtgattag cggttcccag acgtgaaaga gcagcatcat caaaagtcct
      541 gagcaggctg atactagcct gtcgcacggt gcggaattca tttaccagat ctttcatgct
      601 gcgttcattg gcacctgatg cagttacata atcatcctgt tcaaatccgg gcagagaggt
      661 ctgttctcct cttgcgatgc acaaagcacg ataacacatg atgcgttcgc agtccaccag
      721 gtgattgatc acctctttga tggtccattt tccggttgcg taggcatgac cggcctgatc
      781 atctgacagt cgcagaagca gtgtttcagt atcgttgaac tggttggtga tcagatctac
      841 cagatcgcca gcaggcacca ggtcaaaata ggtcttctga taagggatga aggttccctc
      901 ttccggttgt ttccaatttg ccatagagct aatataccaa caaaaaaagg tccgccttgc
      961 ggcggacctc tcctattcat gcacttgttg tcagtgcaca atgatcagct gtccgttcag
     1021 gacacgacca ttgcctacat actggatgta gtagataccg gcgggatagt cagctatatc
     1081 cagcaacagg gtattacttc ctttttccag tttcatttct tccaggcgca acggaacccc
     1141 aagctcattc agtacttgca ggtggccgct ctcctcttca ggcgagtcaa tacgaacggt
     1201 cacttcattg gatgcagggt ttggctgaat gcgcaggatc tgttcatcct cttctgctat
     1261 tctcaatggt ggatcagcat cttttacccg gaaggtcatg gtgcacttat cgcatttgac
     1321 acctccgcaa atggcagtca tctgcacctg atagttaccg gctgctgtcg gagtgaaatt
     1381 aacaggcata ccaaaaccac tggcataagg cacaccattc tgtctgatgg tccattgata
     1441 gaccgcatca caggcacctg aacaattata tacaccattc aatgagatcg catcaaaagc
     1501 attgaccgtg aacttactgc cacacgggcg attgatccag gtcttgaatg gagtactgac
     1561 ggtgaacaat ccccattctc cgcaggtaca atcggccgtt accacttcaa agtacatggt
     1621 acaggtatcg caggaatttc cctggcaata gcctactaca tcaagcgtgt acagaccggg
     1681 attcagcaga ctgaatgatg gatatccgga tccgtctatc gctgtaccgg ggcccgtgag
     1741 tgtccaggaa tgatctgcat cacaggaggt gccgtccaat gccaggcatg aatatccacc
     1801 actgatgaaa tcgatcacgg ttcctgcagg gatactgttg taaacagcac cacagcttgc
     1861 aataagagaa tagtctggca atgcacccgg ctgatccaca ttggcaatga agttctccca
     1921 tttattacat tggcaggtat cacattcggg aattggaatg caaatgctgt ccatctcatg
     1981 gcaacaccat ccatcatagt ctgccagtgt caggtcgaaa caaatgttcg tgccggcggg
     2041 aagcccgagt ggatcaatga tcacagttcc tgtatatgaa cctccccatg caactgcaga
     2101 cggcaggatg accgggttcg gggtgatgtt cacaaatggc gtcacactgg tgaacaccag
     2161 catatagaca tcattcgggt tgttgttggt gaaggtgtaa ttgtatgcat aagtaccatc
     2221 tccgagacag gtgatatcgc cggagatctc tccgcatgga ttcacgttcg gagtcgggca
     2281 ggtaaattcc agcgtatcag agcaggccac tacctggttg ccatcggcat ctgtatagag
     2341 ccagttgacg gccaccagtt gcaccggtgg tgccaccact gtaacgttta aacacacatc
     2401 aaaaagaccg ggtatggatc ccatcggcac aaagcctcct gttggctgca catacatggc
     2461 agatgccgtt ccataggcag accaggtacc ggtataggag ggacctacca tgagcgatgc
     2521 aatgctcacg ccgggagata gcggtgctac ctcgatacca ctgaagaagc tgccattata
     2581 tccatttgtc aggttgacag ttgcacaaca acctgcactg gtatctgcgg taaccacgag
     2641 gttatcacat ggaccgcagg gcggtaaagg gatacaatac atcgtggtat catgacaaca
     2701 ggtatcagcg ttatagagtg acgccaggaa acacagttct gcaggcagtg ggctgcctat
     2761 aggatacgat acattgatgg agataggtgc agaagtggca ccggtagcaa gcgtaagcgg
     2821 caccacgaaa gacggtgtca gggtgaacgg catattcaca tcatgcagcc acaaataggt
     2881 agaagtagcg gcaccaaggt tcgttacctg aatttccatc gtgtaagtgc catcgtcatt
     2941 gcaccagatg gtatcttcta tggtaaagca ttctacttcg cactccagtg gtgtgtactg
     3001 gaattcagaa ctgtccacac atccataaat ggaagttgct accagtttga tctgatacgg
     3061 aacaccaaag gtgggcaggt tggaggtatt caactccaga tcctgtaatg tgccggcagg
     3121 tgtattgtca atataccact ggtaagacag catgcccgga gggccgtgaa tgatctccgg
     3181 tgtcggatag atacaggatg aatcacaacc tatcggcagc atactcaggt caggcagcgg
     3241 gaagatattg gcatatgctg atgcacttgc tgtacatccc actgcatcgg taacagtgac
     3301 cgaatagttt cctgccgcat ttacgatggt cgtgctggtc atatcgccgt tgctccaatc
     3361 ccacatcaca gcagtggcag aattggcggt cagcgtaacc ggtgtaccgg agcaaacacc
     3421 acccggatca gaactggtca caaacactgt tggcgattgt tccaccatga tcacgacaga
     3481 ctgataatca gaacaagggg gtgtcgggcc ggtggtggtg gaaacggtca gttccactgt
     3541 atgcgggccg gcgccaagtg ttcctccggg aatggtcagg gtggggccag cgaaatacgg
     3601 aataccatca actttccagt cccaggtata tagcccgtaa gtattggact gaatgatgat
     3661 atcatcatca gaacacaaaa gcggcgggaa ggtgaaggtg ataacaggct gaggatagat
     3721 ggtgatcatg gccggcagac tgttgatgat acaaccgtcc acatccacca cttccaccga
     3781 ataatttcca ctcaatgtag ccgtgtaggt gcttcctgtg gcacccggaa tagcggtgcc
     3841 attgttgaac cactggtagg aagtatagat cactgcaccg ggaacagaca gggtggttgg
     3901 tccgcatgat ggtgaagcat acaacaacgc aaacgaagca ggaggcgcaa caacggtgat
     3961 attaccggtg gctgttgtct gacatcccgc cgcatctgtt gcggtcaatg tccaggtgta
     4021 ggtattcggt gtgtcatagg tatgttctgt attctgctga ccggaagtaa atccgtcgcc
     4081 gaaatcccat tgccagtcga tgacgttacc gcccagtacg gtagccgcga aggcaaccgg
     4141 tccgttgcag gcagaaggtg aaatggtggt actgacggcg ggtccgccaa tggtgatgtt
     4201 ttgtgtatag ctggcttcgc aacctgctgc tgtatatacg gtcagggtaa cagggtatgt
     4261 gttgggaaca gcataggtat gtacgccggg agcactgccg tatgtgagcg gactaccatc
     4321 gccccagtcc cattcccatg tgactatgct atacggtggc aggaagctgg cattgtccac
     4381 tacggtcagc gtattgcaca ccacttccca tgcaaaatct gctgctaccg gaacagctac
     4441 aacggtatct tccggcagtg gcggacaaca ggtcggatca tagtaactgt aatacacatg
     4501 gtagtatccg gcctggttat aggaatgtac caccgatgtt ccggtaccac tgaaaccgtc
     4561 gccaaaagtc cagttgaaca caccgcccac acaggtggag ttgctgctaa aggtgaactc
     4621 attacaatca ggcgaactct gtacaaaagt gggtggaaag agatcgcagg cacacagcgg
     4681 agtgatggct gtatcacagg tatcttcaac aggaacagga cagaacacat cataggtatc
     4741 agtattggta cacccgttca tatcagtaac tgtaacactg taggtaccca ggttgctgat
     4801 ggttatcgga ttggtagtgc cacctgttga ccacatgaaa gtatagccat tgtccagtgc
     4861 atacagatct atgctgttgg tcatggcagt accatcatcg ttgcaaataa cattgtcagg
     4921 tgtagaaata aatgcctccg gttccggata ttcaaagaca tctgtgtagt caatattggt
     4981 acagccgttg ccgtctgtgc ccaccacata ataactgccc tgttgggtga cagttgcaga
     5041 cacaggcaca ctgttttcat caaaccactg ataagaattc cctccggttg cggtcagtgt
     5101 aaaggaagtt cccggacaga acgcaccgcc ggtggccacg atcacaggtc ttggataaat
     5161 atcaaaagtg atcacatctg taccggtact ggtcagacag gtggtctctg taacggtcag
     5221 tgttactgtt tgcgtggtgt tggaggcgtt accatgccag agaatgtcaa ccgtgccggg
     5281 aagcaggccc ggtgtaatgg tacccgctga aggcggagaa atggtccatt ccacgtcaga
     5341 caatccggga taactcaggg tagctgaata ggtaacggtc tgatccggac aaacatttgc
     5401 tgccgatgtg gtgatgacag gagctggtgt tcccggagca tccagggtaa tgctggcaga
     5461 gttgctgcaa ccattctcgg ttaccatgac cgacagtgta ccagttgtga aggcaacagg
     5521 cagtgtgagg tcaaacgaat tgtcataacc aaaaatggat cctgacacat ctgtgaaggt
     5581 ccagacgata cttgccgttg gcgaatagcc gctcaccgta taggtatagg tgcctccggg
     5641 gcaaacaact gaagggcctg agatgaccgg tgttgcaggt agtggattaa taacgatact
     5701 gatggaatca ggcgagttgc atacagatgc cgatgatgcg atgatatgat acacacccgg
     5761 cccggggaag gtagtacttg ctgatgtggt cgctgttccg aaaggagtaa tcggcaaatt
     5821 gtattcccag tcggcgggat agttggaact aaagaacaca gtagatccat cgcatgcaga
     5881 ggcaggacca ctcaaagtca gcaacggtgc agtctgaatg atcagtgtat cggctcccac
     5941 acaatccagc aggtcgtgtt ccatggaaca aataagctgg taggtaccgg cagcagagaa
     6001 ggtaatatcc tggatataag gaggcatgcc atacggcagc gttacgggaa taccagaggg
     6061 tggtatcagt gtccaggtat aggaagcacc gggcacataa ggtgctgaat aaggttctgt
     6121 cactccctga caaacgacga ccgggccggt aatatcaaaa gtaccatcag gcatgatggg
     6181 tacggtagcg aagaccgtat ctgcgcacaa gcccggagaa cagcctccta ccagcagact
     6241 gatggtacct accggacctg caccccagtc aacggtgatg cagggttgat tcggagcaga
     6301 ggtgatcaca cctccgtcca catcccagtc gtaggtactg cacactgcat ccgtgcaata
     6361 ctctgcaccc acggtattgg cacaaacggc gctgatacag aaaatattcg gcccctctgt
     6421 attgtccact atcacctcta cggtatcaga ggtactgcag cattcagtgg aagcagtgac
     6481 caccacctga taggtaccgg aagtaatata tgtatgcgtt tcaggcgaag cgccggaatt
     6541 gaccacaatg gtaccatcgc caaaatccca gctgtaggtt atgaatggcc caccggtcac
     6601 actcagtgaa aaggctactg actgattcag acaaatggta ctgatgcccg ggctgacggt
     6661 aatggttccg ttgggatagg aagacacccc aaacacttca tcggttccgc tgcatccggc
     6721 agggttggtt tctgtgaccg tgagccggcc gcagggaaaa tctccgcctg tcatggccac
     6781 atcccagatc acatagacgg ttgactggtt atcgggcccg acgatggtac cgccgctgat
     6841 ggtccacaga taggtattca gcggatcatc aggtaccgca tattccacca gttcattgat
     6901 acaaaaagtg tcgcatgcag ttactcctgc gcctgctcct tcaataccct catgtgtatt
     6961 gcagtcattc tcgagataat cgatcaccgg agccggggtg gcatacacct gaacggtcag
     7021 attctctgat gcggcaccgt tgtctgcgat cagggtgtag gtacccggcg gtacattcca
     7081 tgtcagaaac agggaattac ccgacgggaa agtggccgga ctaaccgggg tgatggtcca
     7141 ggtaagtgta ccggacaacg acgaataata ggtttccgtc tcgtcgatgc acacgtcctg
     7201 atcgcccgtt atctgcgcca tgaggccaga tgagacaaaa aggatcatca tgcatagtaa
     7261 caggatcttt tgcaggttct tcatgcgtga acttttagag ggtaagtgtt agtagtgtgc
     7321 acacctgtgc agtttaattc ttgctaaagg actacctttc ctgcaataaa ttcactgcta
     7381 aatgaaaagt gtcgcccttt tttgtgtaac tgtcctgata ggcatgacaa cgaacctcag
     7441 cgcccaaagc catttggaga taccctatat agaagtgacc gggaccgcaa aactggaggt
     7501 ggtgccggat gaattctatg tgaatatcac tttgcgtgaa cggtatgatg gccgcgacaa
     7561 gatcaccatc gacaaacagg aagaagatct gcgaagggca tttatcgcca tcggcattcc
     7621 tgatacccag ttctacctgg ctgatgtgga tgcagattat atgaaggttc ggtggaatac
     7681 caaagatgtc atcacccaga aaaactatac cctgaaagtt accaccccta ccctgctgag
     7741 caaagccttc caggctatgg atgaaaaaga gatcaaagat gcctatctga gccgggtgga
     7801 tcattctcag atggacagct tgcgccggga agtaaggatg gaggcagtcc gggcagcgaa
     7861 agataaggcc acttacctgc tggccgccat agataccgat ctgggtgctc cgctgatagt
     7921 ccgggagcag gagatgtact atggtaatcg caatatggaa atgtacaacg tgaggatgga
     7981 cgatatggat aaagcatcag agccactgcc ggagctgcag tttcagaaga tacagctgga
     8041 gcaatcagta tttgttcgtt ttgccattgc cgctgcacaa taagcatgaa cccgatacgt
     8101 ctgtaagctg ttagcctgat actttcctat ggatctgctt tccattctca tccgtttgct
     8161 gagcaccgga ctggcatttt tcctgcctga cctactgttg tatggtgcgg taaaaaaagc
     8221 ctggaacagc cggggttgga agatctatct tatatccaac cttgtcctgt tcggcattgt
     8281 tttcctgtct tccttcctgc ttcgcgaaag cacgctgata ctacaacgca tacagttgta
     8341 tttctttggt ttgttcgtgg ccattctatt tgccaaggtg ttcacggggt tgttgttgct
     8401 ggtggaattg ttcatccgtt ttatctacag cctgttcacc aaaaagaaaa gaaagtcgga
     8461 agatgcggaa gcaaagaccc tgatctcccg ccgacagttc atcgccaaag gagccatact
     8521 gaccggtttg cttcccatga ccggcatcat ctacggtatg gcaaaaggac ggttccgttt
     8581 tacattacat gaagtcgata ttgccatctc caacctgcct cccgcatttg atggatttac
     8641 catcacccag ctaagcgatc tgcatatcgg ttctttcgat cacagtacca tgcacgaact
     8701 ggcagatatg gtccaggaag taaatgccct tggctctgat gccatattct ttaccggcga
     8761 tatcgtgaac agccgtgccg atgaaatgga tggctggtat gaactgttcc ggacattcca
     8821 cgccgaaacg aaactgtcca ttctgggcaa ccatgattat ggcgattatg tcaactggcc
     8881 cgacgaagcc gccaaagaaa gaaacctgga ccaggtaaag gccattcatg gcaaactggg
     8941 atttcagttg ctgctgaacg aacgtacggt catagaaaaa gacggacagc aattacatat
     9001 tatcggtatc gaaaactggg gtaaaggcga cttccccaaa ttcggagatc tgggcaaggc
     9061 agctgccgga ttgacggccg atgactgcat gatattactg tcgcacgacc cctccttctg
     9121 ggatgcacat gtgctcgatt cacccttcca accggaactt accctgagcg gacatacaca
     9181 tggtttccag atgggtatcg aaacacctgc tttccgtttc agtccgagtc aatgggtgta
     9241 ccgtcagtgg gccgggctct atgccgaagg caggcaacaa atttatgtga accgtggtct
     9301 gggtacagta ggctatccgg gaagactggg catctggccc gagatcacga agatcagatt
     9361 gatcagagaa taagaaaa
//
LOCUS       DAOMAX010000086         9332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1028_length_9332_cov_4.320470, whole genome shotgun sequence.
ACCESSION   DAOMAX010000086 DAOMAX010000000
VERSION     DAOMAX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1028_length_9332_cov_4.320470"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK742_06100"
     CDS             complement(<1..195)
                     /locus_tag="PK742_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76196.1"
                     /translation="MSNNAKFQAAPQQESTSGSSGVDLQLLLLFTWKNRYIILLIMML
                     SLLAGYMVNKYSVPRYSAKSV"
     gene            complement(204..971)
                     /locus_tag="PK742_06105"
     CDS             complement(204..971)
                     /locus_tag="PK742_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HQU76197.1"
                     /translation="MKWYCHAVLFGAVILALSSCKTLNPNAMARYDKDYAFTVLTDTM
                     PQDYPLAPGDRFDLYIFSNNGYLLVDRSISGNNPAQQNLQQNTLFYDISQDGSCTLPM
                     IGEQQLAGLTEKEAEALLSDLYKEYFLEPYVQLRVINRRVTVFRGNDLAKVVEMPSLN
                     MTVMEAIAAAGGIPPTGKAYRITLIRNAGRTDQSVSLIDLRDPEGLEQAGKLVRPNDV
                     IYIEPTINTAFFKEIAPIISAVTSIFILYTYFDTISQ"
     gene            complement(1098..3044)
                     /locus_tag="PK742_06110"
     CDS             complement(1098..3044)
                     /locus_tag="PK742_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQU76198.1"
                     /translation="MITSLQRFIIHRYSGRILSKWIVLLHDMVLVAAAFGLAQLLRFN
                     FKMEGIRNDHFVTQLSISCLVYLAAFFLFRSFSGIIRHTGLEDVKRILNAGLLSMTTL
                     VGLTILVRTSSLTSGMTISLAVIIIHFLLTVVFLTGSRFLIKLFYHNAVKKRSAFKRV
                     VIFGAGYSGMMAKNSMESDTASNYKVSAFLDDDPQKAGKFLHGIPVFLPEAGFRDFLH
                     KFPDTELIIAIPNLTPARKSQIIEHCLNVGIKVKSVPPIGNWINGELSVQQIRNVSIE
                     DLLNRAPIQLERSMAAKEIYSKVVMITGAAGSIGSEIVRQVMNLNPAKLILIDQAETP
                     MHDLELEVAPLRRRFNTDVQFVICDITNLPKLTRLFEFYRPQIVYHAAAYKHVPMMER
                     NPGEAVLTNVFGTKHMVDLAIVYEIEKFVFISTDKAVNPTNIMGATKRAAEIYVQNQS
                     RYESSKTVFVTTRFGNVLGSNGSVIPLFMRQIEQGGPVTVTSKDVTRFFMTIPEACQL
                     VLEAGAMASGGEIFLFDMGERVRIYDLAVKMIRLAGRVPDKDIFIEEIGLRPGEKLHE
                     ELLNAAEDNLPTHHPKIMVGRTREFESEWVHRCMERLRVAYADNNTIKLVLALKEMVP
                     EFKSLNSDFQQLDREATMEVAATA"
     gene            complement(3214..4248)
                     /locus_tag="PK742_06115"
     CDS             complement(3214..4248)
                     /locus_tag="PK742_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQU76199.1"
                     /translation="MSGKQGFKCLLLGYGHIGKRHAAVIAEHPETELSGIADLTDVRS
                     VAGYPSDIPWYPSWKDALANSGGHDLVIIALPNGEHIPAALAALKSGYHVLIEKPMGL
                     TALSCQQVLELAEQQKKQVFVVKQNRYSPQVQWLKDLVDSGKMGKIHMIQVNCFWNRD
                     DRYYLQQGHPWRGKLTSDGGVLFTQFSHFIDILYWIFGPLMVESAHMENLAHSTTTEF
                     PDSGSFRFRTAGQALGFMQFSTAVWNRNLESSITILGDSGTIQLGGQYMDTVLHCHIP
                     DYILPQIPSANPPNLYGNYSGSAANHVQVLEQVVMALKGEKSAATNGQEGMEVVRIIE
                     TVHRLANHPI"
     gene            complement(4245..5222)
                     /locus_tag="PK742_06120"
     CDS             complement(4245..5222)
                     /locus_tag="PK742_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76200.1"
                     /translation="MNLIPVLIALLVIMVTTLIWIFIARRSEIYVHRKERSVRKVITG
                     MGVIFTVATILSYWQKDFSDYTVLIAVSLLAVVSLLDDIMDLSIGLRLFIQLIVAGVV
                     YMAYPVLDPWWMILLLVVWMNMFNFMDGSNGMLGLYALVVLASVLFSSGLPVQLILSG
                     GSLYHMSFILILALLVFLAGNLRKHAVWIAGDAGSVVLGLLVIWILLTDRSGTALQAV
                     DEANFSWLFIPVSCALFVTDTGWTLIRRIYLRQPVWQRHRLHAYQMLIYHKDKNPVLI
                     AFAYAVLQLLVNMLFLISGGGVWMAIGIFVVLSAAWWMINRNWPEKSDL"
     gene            complement(5219..6319)
                     /gene="wecB"
                     /locus_tag="PK742_06125"
     CDS             complement(5219..6319)
                     /gene="wecB"
                     /locus_tag="PK742_06125"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQU76201.1"
                     /translation="MNQVNIDLIAGARPNFMKIAPIIRALQEAEKNGSKLQYRLVHTG
                     QHYDRNMSDQFFEQLGIPEPDVNLGSGSGTQAEQTARIMMAYEKLLMEQPADLCLVVG
                     DVTSTLACSVAAKKLGIKVAHVEAGIRSYDLTMPEEINRMVTDSITDYFFTTSEVANA
                     YLRRTGVEQDRILFVGNTMIDTLLHHRKDFRKPEIWDEAGLEQGKYIVMTLHRPSNVD
                     EESTLIDLIRTIIDHSRNLPLVFPIHPRTRKVLEHSGLDHPRLLQVDPLGYLEFNYLV
                     ERAAAVITDSGGITEETTVMGVPCMTLRANTERPETIDIGTNELLGTNPDAIPASMEK
                     LFSGEWKKGSIPPLWDGHTAERIVEHLENIFQ"
     gene            complement(6316..7446)
                     /locus_tag="PK742_06130"
     CDS             complement(6316..7446)
                     /locus_tag="PK742_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQU76202.1"
                     /translation="MQNKMWLSSPHMCGREEGYVKDAFDTNWIAPLGPHVNGFEQGLS
                     ARMLEHPVAALSSGTAALHLALILLGVERDDVVICQSFTFSASANPIVYQGAVPYFVD
                     SEADTWNMDPDLLEEAISRLVTVGKRPKAVVVVHLYGMPAKMDRITEICERYQVPVVE
                     DAAEALGSALHEKPCGTFGRFGVLSFNGNKIITTSGGGALVCPDDAFAERARFLATQA
                     RDTAPHYQHSVIGYNYRMSNVCAAIGRGQLEVLDQRVEQRRKNFAYYQETLSDLPGIS
                     FLSETAGSFSNRWLTCITIDPDHSGGVTRESVRLALETQQIESRPLWKPMHLQPVFAN
                     APAMSTGVSERLFNTGLCLPSGSNLTSEDLSRVVEHIRKNWK"
     gene            complement(7448..8083)
                     /locus_tag="PK742_06135"
     CDS             complement(7448..8083)
                     /locus_tag="PK742_06135"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76203.1"
                     /translation="MNRKLIIVGAGSVGLHLAANADMYGIKEHLIGFVDDDPGKQAGS
                     YYGYPVLGTLSYVLEQQDIDVVIGIAFPDVKKKIVKKLSGNDSLGFPGLIAKAHSWIS
                     ADCSIGKGVIIYPGCSINYGSVIGDFVVMNMNCALGHHATVGRYSSLAPGVCFGGHTT
                     VGEGVDIGIGAATIQNTLIGDGAVIGGQSMIIRNVEAGETVVGVPAKNKSI"
     gene            complement(8098..8739)
                     /locus_tag="PK742_06140"
     CDS             complement(8098..8739)
                     /locus_tag="PK742_06140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQU76204.1"
                     /translation="MSLYKNYIKPAMDRIAAFLLLLLLFPLLLLIMLIQWFILKDRIF
                     FIHPRPGLHEKIIHVIKFRTMNEKKDESGALLPAMQRITPWGAFLRRTSLDELPQLIN
                     VLKGDLSFVGPRPLEVRYLPYYSEQQRKRHLVKPGITGLAQINGRNAIGWEEKFKYDL
                     RYIEEISLMTDLRILLGTVRKVLAAEGVNQDTAQTVLPFDQYMNRKRSDDQVG"
     gene            complement(8744..>9332)
                     /locus_tag="PK742_06145"
     CDS             complement(8744..>9332)
                     /locus_tag="PK742_06145"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HQU76205.1"
                     /translation="ALGQCGWGIEVISKKEFSAEQLVSHMEQKGYDLAEVFIDQHEVL
                     QELSPSGLNTLRVITQLNDHGGVDYLGARLRITINSQVDNMASGNMAAPVDLNTGVVS
                     GPAVFSDITKEPVHVHPISGLTIPGLQLPHWPAVLRMTKEAALLHPENRSVGWDIALT
                     PAGPELIEGNHNWCKLLWQLPVQTGLKDVLLTYLK"
BASE COUNT         2434 a         2349 c         2067 g         2482 t
ORIGIN      
        1 aacagattta gcagaatagc ggggcacact gtatttgttg accatgtatc ctgcaagcag
       61 agaaagcatc atgatcagca gaattatata tctgttcttc caggtaaaaa gaagcagcag
      121 ttgaagatct actccggatg aaccggaggt gctttcctgt tgcggtgctg cctgaaattt
      181 tgcattgtta ctcatgcgtt ccgttattgg ctgatcgtat caaagtatgt gtacaggata
      241 aatatgcttg ttactgcaga gatgatggga gcgatctcct tgaagaatgc agtattgatg
      301 gtgggttcaa tatagatcac atcattgggt ctgaccagtt tgcccgcctg ttccagacct
      361 tcgggatcac gcagatcgat caggctgaca gactgatctg ttcttcctgc atttctgatc
      421 agggtgatgc gatatgcctt cccggttggt ggaatacctc cggcagcagc gatggcttcc
      481 atgacggtca tattcaggct gggcatttca accacctttg ccagatcgtt accgcgaaat
      541 actgttactc ttctgttgat cacacgcaat tgcacataag gttccaggaa atactcttta
      601 tacaggtcag acaacaaagc ttcagcttcc ttttctgtaa gaccagccag ttgttgctca
      661 cctatcatag ggagcgtaca agatccatcc tgactgatgt catagaatag tgtgttctgc
      721 tgaagatttt gctgagccgg attgttgcct gagatgctgc gatctaccag cagatacccg
      781 ttgttggaaa aaatatagag gtcaaagcga tcgccgggcg ccagcggata gtcttgcggc
      841 atggtatctg tcagcactgt gaaagcatag tccttgtcat acctggccat ggcattcggg
      901 ttcagtgttt tgcatgagct caaagcaagt atgactgctc cgaaaagcac ggcatggcag
      961 taccatttca taggttcatg tgattttggt aaggacttac aggcaatgat aggtgatttc
     1021 atgccgaagg gctctgcaac atccacccgg atgtgtttcg gctgtccaaa attactacaa
     1081 cagaaatcct gttttcatca ggcagtggca gccacttcca tggtagcctc ccgatccagt
     1141 tgctgaaagt cagaattcag gctcttgaac tcaggcacca tttccttcag tgcaagtacc
     1201 agtttaatgg tgttgttatc tgcataagct acacgaagtc tttccataca acggtgtacc
     1261 cattcagatt caaactcacg tgttctgccc accatgatct tagggtggtg tgtaggcagg
     1321 ttatcttctg cggcattgag taattcctca tgcagtttct cgccgggtct caggccgatc
     1381 tcttctatga aaatgtcttt gtctggaaca cgacctgcca gtctgatcat cttcacagcc
     1441 agatcgtaga tgcgcacccg ttctcccata tcaaacagga aaatttcacc accactggcc
     1501 atcgcaccgg cttccaatac cagctgacag gcttccggaa tggtcataaa gaaacgtgta
     1561 acatctttac tggtaacggt taccggacct ccctgttcta tctgacgcat gaaaagcgga
     1621 atgacagagc cattagaccc gagcacgttt ccaaatctgg tggtcacaaa cacggtcttg
     1681 gatgattcat acctcgattg attctgtaca tagatctcgg cagcacgttt tgttgccccc
     1741 atgatattgg tagggttaac agccttatca gtggaaatga atacaaattt ttctatttca
     1801 taaacaatgg cgaggtccac catgtgtttg gttccgaaaa cgttggtcag taccgcttca
     1861 ccaggattgc gttccatcat cggcacatgt ttgtatgctg cagcatggta cacgatctga
     1921 ggacggtaga actcaaacaa ccgggtcagt tttggcaggt tggtgatatc gcagatcaca
     1981 aattgcacgt ctgtattaaa tcgtcttcgc aggggtgcca cttccagctc caggtcatgc
     2041 atgggtgttt ctgcctgatc gatcaggatc agcttggccg gattcaggtt catgacctgg
     2101 cgcacgatct cgctgccaat agagccggct gcaccggtta tcatgaccac cttgctgtag
     2161 atctctttgg cagccataga tctttccaac tgaataggag ccctgttcag gaggtcttct
     2221 atacttacgt ttctgatctg ttgtacagaa agttccccgt ttatccagtt cccaattgga
     2281 ggaacgctct tcaccttgat acctacattg aggcaatgtt caatgatctg gctttttctg
     2341 gcaggagtca gattgggtat ggctatgatc agttcagtat caggaaactt atgaagaaaa
     2401 tcacggaagc cggcttcagg aaggaagaca ggaataccat gtaaaaactt acctgctttt
     2461 tgcggatcat catccagaaa ggcagatacc ttatagttcg aggcagtatc actttccatg
     2521 gaatttttgg ccatcatacc actgtatcct gcaccaaaaa tgaccacacg cttaaatgct
     2581 gaacgtttct tgaccgcatt gtgatagaac agcttgatca gaaatctgga tcctgtaaga
     2641 aagacaacgg tcagcagaaa atgtatgatg atcacagcca ggctgatggt cattccactg
     2701 gtcaggctgg aagttctgac caatatggtc aaacccacca gagtggtcat agacagtaat
     2761 ccggcattga gaatgcgttt cacatcttcc agacctgtat gcctgatgat gcctgaaaag
     2821 ctcctgaaca aaaagaatgc tgccagataa accagacagg atattgacaa ctgtgtgaca
     2881 aagtgatcat tcctgatccc ctccatttta aaattgaacc tgaggagctg ggcaaggcca
     2941 aatgccgcgg ccaccaatac catgtcatgc agcagaacga tccacttgga caatatcctg
     3001 ccagagtagc gatggatgat aaaacgttga aggctggtga tcataatctt ctgggttaag
     3061 cgtgttcgat cagtgtgtgc ctttcatagt ttgcaaagat gccaccataa agtgacttcc
     3121 ttattaacaa atgtcaaaag ataagttcaa gttattgtac tcatttgact acaatatgtg
     3181 acatagccgt tatgcctgcc ggtatcagat ccgtcaaatg gggtggttgg caagccggtg
     3241 taccgtttca atgatcctga ccacttccat tccctcctga ccatttgtag cggccgattt
     3301 ttcgcctttc agtgccatca ccacctgctc cagaacctgg acgtgattgg ccgctgaacc
     3361 actgtaatta ccatatagat tgggtgggtt tgctgacgga atttgaggaa gtatataatc
     3421 cggtatatgg cagtgaagta cggtatccat gtattgacca cccaactgga tcgttccgct
     3481 atcgccaagt atagtaatgg agctttccag gtttctgttc cagacggctg tactgaactg
     3541 catgaagccc agtgcctgac ctgctgttct gaacctaaaa gatcctgaat caggaaattc
     3601 ggtagtggtg ctgtgagcca ggttctccat atgtgctgat tccaccatga gcggcccgaa
     3661 aatccaatag agtatatcaa tgaaatgact gaactgggtg aaaagcactc ctccgtctga
     3721 agtcagcttt cccctccacg gatgaccctg ttgcagataa taccggtcat cgcggttcca
     3781 gaaacaattg acctgaatca tatggatctt gcccatttta ccggaatcca ccaggtcctt
     3841 cagccattgc acctgcgggc tgtacctgtt ctgtttaacc acaaatacct gcttcttttg
     3901 ttgttcagcc aactccagta cctgctgaca cgacagggca gtaagcccca ttggtttttc
     3961 aatcagcaca tgatagcctg atttcaaagc agccaaagcg gccggaatat gttcaccatt
     4021 gggaagagca atgatgacca gatcatgtcc gcctgaattt gccagggcat ctttccagga
     4081 aggataccag ggtatgtctg acggatatcc ggcaacagat ctcacatctg tcaggtcagc
     4141 aataccagat aactctgttt ccggatgctc agcgatgact gccgcatggc gtttgccaat
     4201 gtgaccgtaa cccagtaaaa gacatttgaa cccctgttta ccactcatag atctgatttc
     4261 tcaggccagt ttctattgat catccaccag gctgcagaaa ggaccacaaa gataccgatg
     4321 gccatccaca ctcctccacc tgaaatcagg aagagcatat ttaccagcaa ctgcagcacg
     4381 gcatacgcaa aagctatcag caccgggttc ttgtctttat gatagatcaa catctggtaa
     4441 gcatgtaacc ggtgtctttg ccagaccggt tgtcttaaat agatccttct gataagtgtc
     4501 catccggtat ctgttacaaa caatgcacag gaaaccggaa tgaacagcca tgaaaagttg
     4561 gcctcatcca cagcctgaag agcggttcct gatctgtctg tcagcagaat ccaaataacc
     4621 agtaagccca gtaccactga gcccgcatcg ccggctatcc agaccgcatg ctttctcagg
     4681 ttgccagcca gaaaaaccag caatgcaaga atcaggatga atgacatgtg atataaagat
     4741 cctcctgaca gaatcagttg tacgggcaaa ccggaactga ataatacact ggccaatacc
     4801 acaagcgcat acaaacccag cataccattg ctgccatcca taaagttgaa catgttcatc
     4861 cagaccacca gcaataagat catccaccac gggtccagga ccggataagc catatagacc
     4921 acacctgcca cgatcagctg aatgaacagt cgcagcccga ttgacaggtc catgatgtcg
     4981 tcaagcaaac tgaccaccgc cagtaaagac acggcaatca atacagtgta atcactaaaa
     5041 tctttctgcc aatagctcag gatagttgca acagtaaaaa tgacccccat tccggtaatg
     5101 acctttctga ccgacctttc ttttcgatgt acatagatct cagatcttct tgcaatgaaa
     5161 atccagatga gggtggtcac catgatgacc agaagtgcga tcaatacagg gatcagattc
     5221 actgaaaaat attttcaagg tgttcaacta ttctttctgc agtgtgacca tcccacaaag
     5281 gcggaatgct tccttttttc cattctccac tgaacaattt ttccatactt gcaggaattg
     5341 catcggggtt ggttcccagc agttcattcg tacctatatc aatagtctca ggcctttcag
     5401 tattggcccg tagggtcatg cagggaaccc ccatgacggt tgtctcttct gtaattccac
     5461 ctgaatcagt gattacggca gcggctcttt ccaccaggta gttgaattcc agatagccaa
     5521 ggggatcgac ctgcagaaga cgtggatgat ccagccctga atgctccagc acttttcttg
     5581 ttctggggtg gatggggaag accagtggaa ggttcctgct atggtcgatg atggttcgta
     5641 tcaggtcgat caatgtagat tcttcatcta cgtttgacgg gcgatggagt gtcataacga
     5701 tatactttcc ctgctccagt ccggcttcat cccagatctc cggtttacga aagtctttcc
     5761 tgtgatgcaa caacgtatct atcatagtat tgccaacgaa taaaatacgg tcttgctcta
     5821 cacctgttct tcgcagatag gcattggcta cttcgctggt tgtgaaaaaa taatctgtga
     5881 tgctgtcggt caccattctg ttgatctctt ccggcatggt tagatcgtat gaacgtatgc
     5941 cggcctccac atgcgccact ttgataccca acttcttagc cgctactgaa caggccagtg
     6001 tggaggtgac gtcgccaact accaggcaaa gatctgcagg ttgttccatc aggagttttt
     6061 cgtaggccat catgatcctt gcagtttgtt ctgcctgggt gccacttcct gaacccaggt
     6121 tgacatcggg ctccggaatt cccagttgct caaagaactg atcgctcatg ttgcggtcat
     6181 aatgctgccc ggtatgaacg agtctgtact gtaatttact tccgttcttt tcagcctcct
     6241 gtaatgccct gatgatcggg gcgatcttca tgaaattggg ccgtgcgccg gcaatgaggt
     6301 caatattcac ctgattcatt tccagttttt tctaatatgt tcaactactc tgctcaggtc
     6361 ttcactagta agatttgagc cgctgggcaa acaaaggccg gtgttgaaca gcctttcaga
     6421 cactcctgta ctcatggcag gagcatttgc aaaaacgggt tgcagatgca tgggcttcca
     6481 aagtggcctg ctttcaattt gttgtgtttc cagggcaagt ctgacacttt ctctggttac
     6541 gccaccacta tgatccgggt ctatcgttat acaggtcagc catcggttgc tgaaactgcc
     6601 tgctgtttca gacaaaaaac tgatgcccgg cagatcgctc agtgtttcct gataataagc
     6661 aaagttcttt ctgcgctgct ccactctttg atccaggact tccagttgcc ctctgccgat
     6721 agccgcacat acattgctca tgcgatagtt gtatccgatc acactgtgct gataatgcgg
     6781 ggcagtatct cgtgcctgag tggccagaaa tcgtgccctt tcagcgaatg catcatcagg
     6841 gcataccaat gcaccaccac ccgaagtagt aatgatcttg ttgccattga aactgagaac
     6901 tccgaacctg ccgaaagtac cacaaggctt ctcatgaagc gcagagccaa gtgcttcagc
     6961 tgcatcttcc acaaccggaa cctgataacg ttcacagatc tcggtaatgc gatccatttt
     7021 tgcaggcatt ccatacagat gcaccaccac cacagctttc gggcgcttcc ctaccgttac
     7081 cagtctgctg atggcttctt ccagcagatc aggatccata ttccaggtat cagcttccga
     7141 atcaacgaaa tatggaacag caccctgata cactatcgga ttggcacttg ccgaaaaagt
     7201 aaagctctga caaatgacca catcatctct ctccacgcct agaaggatca gtgcgagatg
     7261 cagcgctgca gtacctgaag ataatgcggc aactgggtgt tccagcatcc tggcagaaag
     7321 gccctgttca aatccgttca catgaggtcc aagcggagcg atccagttgg tatcaaatgc
     7381 atcttttaca tagccttcct cacggccgca catatgcggt gatgacaacc acattttatt
     7441 ttgcatacta aatactttta ttctttgcag gaacgcctac aactgtttca ccagcttcta
     7501 catttcttat gatcatggac tgtccgccta tcaccgcccc atcaccaatc agagtattct
     7561 gaatggtggc tgcgcctatg cctatgtcaa ccccttctcc aacagttgta tgacctccaa
     7621 aacaaacacc aggagcgagg ctgctgtaac ggcctaccgt tgcatgatgc cccagtgcac
     7681 aattcatatt catgaccaca aaatcgccga tgaccgaacc ataattaata ctgcaacccg
     7741 gataaataat aactcctttt ccgatggaac aatcagctga aatccaggaa tgtgcttttg
     7801 ctatcaaacc gggaaaaccc agtgaatcat tgcccgaaag tttcttaact atcttcttct
     7861 ttacatccgg aaatgcaata ccgataacaa catctatatc ctgttgttcc aacacatagc
     7921 tcagggtacc cagtacagga tatccataat agctgcctgc ctgtttcccg gggtcgtcat
     7981 caacaaaacc gatcaggtgt tctttgatcc cgtacatgtc tgcattagcg gccagatgca
     8041 gacctacgct gcctgcgcca actatgatga gctttctatt catgttatct atacgtttta
     8101 accaacctga tcatctgaac gcttccggtt catatattga tcaaaaggta gcacggtttg
     8161 tgcggtatcc tggttgaccc cttcagctgc aagcaccttt ctgacagtgc ccagcagaat
     8221 acggaggtct gtcattaatg agatctcttc gatgtaacgg agatcgtatt taaacttttc
     8281 ctcccatcca atcgcattcc ttccgtttat ctgagccagt cctgttattc cgggcttgac
     8341 cagatgtcgt ttgcgctgtt gttcactata gtaaggcaaa tacctgactt ccagaggcct
     8401 tggtcctaca aatgaaagat cgcctttcag cacattgatg agttgcggaa gctcatccag
     8461 cgaggttctg cgaaggaaag caccccaggg agtaatccgc tgcatagcgg gcagcaatgc
     8521 tcctgattca tctttctttt cattcatggt tctgaatttg atcacatgaa tgatcttctc
     8581 atgcaaacca ggtctcgggt ggatgaagaa aatcctgtct ttcagaatga accattggat
     8641 caacatgatg aggagcaata aaggaaacag gagcagcaaa agaagaaaag cagctattct
     8701 gtccatcgcc ggcttgatat agttcttata cagactcaag acatcatttc aaataagtaa
     8761 gtaatacgtc cttcagtcct gtttgaaccg gcagttgcca caataactta caccaattgt
     8821 ggttgccttc tatcaactca ggaccagctg gtgtcagggc gatgtcccac cctacagaac
     8881 ggttctccgg atgcaacagt gctgcctctt tggtcatgcg aagaactgca ggccagtgag
     8941 gcaactgcag gcccggaata gtcaaaccag agatcggatg tacatgaaca ggttctttgg
     9001 tgatatcgct gaacacagcc ggaccactca ctacaccggt attcagatca accggtgctg
     9061 ccatatttcc tgatgccata ttgtccacct gagaattgat ggtgatcctg agacgtgcac
     9121 ccaggtagtc taccccgccg tgatcattca actgggtaat gacacgcagg gtattcagac
     9181 ccgatggtga aagttcctgc aggacctcat gctgatctat gaaaacctcg gccaggtcat
     9241 aacccttctg ttccatatgg ctgaccagtt gctctgcaga aaactctttt ttgctgatca
     9301 cttcaatgcc ccagccgcac tgcccgaggg ca
//
LOCUS       DAOMAX010000087         9126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1072_length_9126_cov_4.733767, whole genome shotgun sequence.
ACCESSION   DAOMAX010000087 DAOMAX010000000
VERSION     DAOMAX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_9126_cov_4.733767"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PK742_06150"
     CDS             <1..848
                     /locus_tag="PK742_06150"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQU76206.1"
                     /translation="GIFTGVSNYQGDLAPVAFKPSETHLAPGVFAKFNFNKFAALKAS
                     YYHGKLSGYDSHYAEPFRQKRNLSFVSNIDEISLFGEINITGYQPLKRKNLLSPYAFL
                     GIGMFHFNPRAEYMGEWYDLQPLGTEGQGTNAYPDREPYKLWQVCVPLGLGFKGAINE
                     HWNVGVEFGWRKLFTDYVDDVSSTYVSKEILIAENGLLSWELSNRYDEVNDGVEWLKD
                     DTYLRGDPADKDWYFFCGFIASYNLDPIIGAMTGFHYFDQTKNRKFRGLRDSRKRMGC
                     PGAPR"
     gene            916..1773
                     /locus_tag="PK742_06155"
     CDS             916..1773
                     /locus_tag="PK742_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQU76207.1"
                     /translation="MKQTLLAAAVALLCLNEARAQQLEVGFSGGISQYYGDLAHQWIH
                     QKTIHPAISLVTTYHISENYAIGLSVGHTTISGNDALSEDDFLQVRNLSFRSPVTEVR
                     GLITWYPLPFRPGSRTWFPYVQSGLSLFRFNPQANYKGEWIALQPLGTEGQGTSTFPG
                     RQPYSLTQIGIPVALGCKVAINEYLFIGLETSLLWAMTDYLDDVSLTYVNPDVLIEEN
                     GLLAYQLSNRTGEFLQAEPLNLGNGSMRGNTESLDQVGYFEVTFSVLIPGVSKSWFDP
                     HRKQLNCSF"
     gene            1937..2899
                     /locus_tag="PK742_06160"
     CDS             1937..2899
                     /locus_tag="PK742_06160"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQU76208.1"
                     /translation="MNRSDLTHTFESGWHNCRHWLVLAWMILSCSQGSVQAQLNVVTK
                     RQFLEFGLVGGTMMYQGDLSHNYYFYVNYKPTFGGFIAYPLNEHFSAKLMYLQGYIAG
                     ADSNALEVPIQTRNLDFFSDIHEWSIQLDMYLLRFNSLKEGSSSFSPYFSTGLTVYHF
                     NPMSTFTDPATGLDTVYALQPLGTEGQGTSAFPDRKPYMLTQVAIPLEFGFKFTPVRY
                     LAIGLTGGVKLTFTDYLDDVSTTYVNPEILINENGMVSYLLSNRTWEVTENGEPLALG
                     NKATRGGADSNDYYGYLAVTLSYLFIPEYKFNVSRKAGIHCPKF"
     gene            2999..3928
                     /locus_tag="PK742_06165"
     CDS             2999..3928
                     /locus_tag="PK742_06165"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HQU76209.1"
                     /translation="MRIRNLLFALLLTSATSSMAQMPLLSWVEIGVFGGVANYYGDLT
                     QDYVVWDESHPAYGGFIKYNFNYRSGLKLDVIHGTISAADANSDRADQLLRNLSFTSD
                     VTEVALVFEYSFPGMFPKELRMPFSPYANVGIAGFHFNPRTFFRGEWYDLQPIGTEGQ
                     GINELDFREPYSLYEFAIPFGIGVKWAFSERWNLGLEYAARWTFTDYLDDVSRTYVDR
                     NLLIEENGILAYQLSNRTGEYLLADNIDFGTNDFRGDPTDKDWYMFVGLTLSRNFISG
                     TDEGFISLDKPGLGCFQPKYVKKHTKKNYRSTF"
     gene            3996..5483
                     /locus_tag="PK742_06170"
     CDS             3996..5483
                     /locus_tag="PK742_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQU76210.1"
                     /translation="MTPGLILLVLGLYFSLLMVVAYLTSRKADQSSFYIGNRNSRWYL
                     VAFGMIGASLSGVTFMSVPGAVGDGGFHYFQIVLGYLAGYLVIIRLLLPLYYRMQLTS
                     IYEYLEQRFGSVTHRSGAGLFLISRVLGASFRLYLVAIVLNKFILEPFGLPFWVAVVA
                     TIVLIWIYSFQGGIKTIVWTDTLQTTFMLLSVVVTLIYITGQLDLNVSETIGTVRNSA
                     YSSMFEWHWQPANSFYKYFLSGMFITIVMTGLDQDMMQKNLSCRNLEESQKNMRWMSI
                     SLLPVNLLFLVLGALLYIFLEQSGTGHAVFGPDCQIAIGDDCIRKDSLFASLSITVFP
                     VWMGIVFLLGLTAAAYSSADSALTALTTSFCLDILQHKEHTVPVRLRYLVHAGFSVLL
                     VLVILIFRNLNNESVINELFTVAGYTYGPLLGLFAFGMFTRRQVKDAWVPVVCILSPV
                     ACYFLNKYDTQLLGGYQFGFELLLVNGALAWMLLYFISKKPEDVR"
     gene            5473..6180
                     /locus_tag="PK742_06175"
     CDS             5473..6180
                     /locus_tag="PK742_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQU76211.1"
                     /translation="MSDKYRKKKMYKTKDWTEFTAESSWRMFKIMAEFVEGFERMDKI
                     GPCVSIYGSARTDPANPYYPLAEKLAARMAQEGFGIITGGGPGIMEAANKGAHSMNGK
                     SVGLNIELPFEQYANPYIDQDKLINFNYFFVRKVMLVKYAQAFIYMPGGFGTLDELFE
                     AITLIQTHKIERVPVILIGEEYWKGLLQWMDKTVLHAERNISPEDMDLFFITDDVEAA
                     VTHVTEFYATSHLRPNF"
     gene            6183..6665
                     /locus_tag="PK742_06180"
     CDS             6183..6665
                     /locus_tag="PK742_06180"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQU76212.1"
                     /translation="MALTSITTLKVGAKAPSFKALDQDGRPFDSKEMLGKTWVLFFYP
                     NDMTATCTIEVCDLRDHYKKLKQAGLEIVGVSRGDVASKKKFSSRNKLPFPLLADEDL
                     KISGKYKVFGDKPFMGKILESIYRTTFLIGSDGKILHIIKQVKAKEASAQILEAVRSL
                     "
     gene            6740..7549
                     /locus_tag="PK742_06185"
     CDS             6740..7549
                     /locus_tag="PK742_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76213.1"
                     /translation="MKRTITFSMLFCLAMLFAWRGYVQAKEDGAPSGSTGAPGDDGTC
                     AQVDCHTGSAVPRSGFISTDIPLAGYVPGQDYSIEVSVSSENRNTFGFQLTPQDASGI
                     KVGSLSVTDADRTKITGLGKYITHKKNGIDGTDGMINWNFTWTAPESPTDLTFYVAVN
                     IADGDGDATGDSIFIANLPVSADPLATAIHEMTIATKDWMTVTPGDQLMFADWVLAEQ
                     PVAEIWNLSGQHVSSFTLDEKSHSVAMLDKGMYLITIPTLYGRLTGRFIRQ"
     gene            complement(7550..8008)
                     /locus_tag="PK742_06190"
     CDS             complement(7550..8008)
                     /locus_tag="PK742_06190"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HQU76214.1"
                     /translation="MRIREIVAISGMGGLFKVEGQKVNGLIVTSLAEGWTKFVSNRQN
                     MFSPLDNISIYTDADNVELADLFLDIHAKMAEHPAPDSNADGAALRNWFLAILPTHDQ
                     EKVYTSDIKKIIKWYHILNEHGIIDDEIKARAEEEASGNKEVTEEKGSEQ"
     gene            8077..>9126
                     /locus_tag="PK742_06195"
     CDS             8077..>9126
                     /locus_tag="PK742_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQU76215.1"
                     /translation="MAFQLTSQYTPSGDQPGAIDQLVNAVNEGEKAQVLLGVTGSGKT
                     FTIANVIQQVQRPTLILSHNKTLVAQLYGEFRQFFPENAVEYFVSYYDYYQPEAYIAT
                     TDTYIEKDLSINDEVEKLRLKTTSSLQSGRRDVIVVATVSCIYGIGNPAEFKESIVRV
                     SQGDQVTRNFLLHRLVEALYARTLTDFRRGTFRVIGDTVEVYPAYADYCYRITFFGDE
                     VEEIEIFDADTGRKTETAEHIAIFPANLYIAPKEILPQIIHEIHEEMDEQVAYFERAG
                     KYMEAKRLKERVNFDIEMIKELGYCSGIENYSRFFDRRKPGSRPFCLIDYFPDDFLLV
                     IDESHVTVPQIGAMYG"
BASE COUNT         2338 a         2058 c         2216 g         2514 t
ORIGIN      
        1 tgggtatttt taccggtgtc agcaactacc agggtgatct tgccccggtt gctttcaagc
       61 cttcagaaac ccacctggct ccgggggtgt ttgccaaatt caattttaac aagtttgctg
      121 cactaaaagc gagctattat catggcaagc tatcgggtta tgacagccac tatgccgagc
      181 cattcagaca aaagcgaaat ctgtcatttg tttccaatat tgatgagatc tcgctttttg
      241 gtgagatcaa catcaccggc taccaacctt tgaaacggaa aaatctgctg tcaccatatg
      301 ctttcctggg tatcggaatg ttccatttca accccagagc tgagtatatg ggagagtggt
      361 atgaccttca gcctctgggt acggaaggtc agggcacgaa cgcctatccg gaccgtgaac
      421 cctataaact gtggcaggtt tgtgtgccac ttggtctggg tttcaaggga gccatcaatg
      481 aacactggaa tgtgggcgta gagttcggct ggcgcaaatt gtttacagac tatgtggatg
      541 acgttagctc tacctatgtc agcaaagaga tcctgatagc cgagaatggg cttctgagct
      601 gggagctcag caaccgctat gatgaagtga atgacggcgt ggaatggctg aaggatgaca
      661 cctacctgcg tggcgatcct gcagataaag actggtattt cttctgtggt ttcattgctt
      721 cctataacct ggatcccatc attggagcca tgaccggatt tcattacttt gatcagacca
      781 agaatcgcaa gttcagaggc ctgagagact cccgcaaaag gatgggttgc ccgggcgcac
      841 cccgttaaag ttttttttca cttgtgacaa ctttttgggg tcattcatcg tatagacgaa
      901 tgaacaacct aattgatgaa gcaaacctta ctggctgctg cagtggcctt gttatgtctg
      961 aatgaagccc gtgcccaaca gttagaggtt ggtttcagcg gcggaatatc tcagtattac
     1021 ggagatctgg cgcaccaatg gattcatcaa aagaccatcc atcctgccat atcgctggtt
     1081 accacctatc atatttctga gaactatgcc atcggtctgt ctgtgggcca tacaaccatt
     1141 agtggtaatg acgccctgtc tgaagatgat tttctgcagg taagaaacct ctcgttcaga
     1201 tcgcccgtaa ccgaggttcg cggactgatc acctggtacc cgctgccttt caggccaggc
     1261 tcccgaacct ggttccctta tgtacaatca ggcctcagcc tgtttcgatt taatccacaa
     1321 gccaattata agggagaatg gatagctctg caaccattgg gaactgaggg gcagggcacc
     1381 agtacctttc cgggaagaca accatattcg ctgactcaga tcggtatccc ggtagcgctc
     1441 gggtgcaaag tggctatcaa tgaatatctg ttcatcgggt tggaaacatc tttgctctgg
     1501 gccatgacag attatctgga tgatgtgagc ctgacctatg taaatcctga tgtattgatc
     1561 gaagagaatg gactccttgc ctaccaatta tctaatcgta ccggagagtt cctgcaggct
     1621 gaaccgctga atcttggaaa cggcagcatg agaggtaaca ccgagagtct ggatcaggtg
     1681 ggatattttg aggtcacatt ttccgttttg attcccggtg tcagcaagtc ctggttcgat
     1741 ccgcaccgga agcagctcaa ctgctctttt tgaagctaat tctgcacact gaagtcatag
     1801 tttagtggat aaaacagggg ctctcccggc ttgcttcatc taattttaag cctgcattga
     1861 tgtcacatga ggtcaaaaga cgtcaaccct ttgcgatgaa ggtcagatta atttcacatt
     1921 cgtaaaatac agttccatga accggtctga cctgacacac acctttgaat caggctggca
     1981 taattgccgg cattggttgg tgttggcatg gatgatactg tcatgctctc agggaagtgt
     2041 gcaggctcag ctgaatgtgg ttaccaaaag acaatttctg gagttcggac tggtgggtgg
     2101 cacgatgatg taccagggcg acctttcgca taactattat ttctatgtaa attacaagcc
     2161 taccttcggt ggcttcatag catatccgtt gaatgagcat ttttctgcca agctgatgta
     2221 tctgcagggc tatattgccg gagccgatag caatgccctg gaagtgccca ttcagactcg
     2281 aaacctcgat ttcttttctg atatacatga gtggagtatc cagttggata tgtatctgct
     2341 gcgatttaac agtctgaagg aaggcagctc ttctttcagc ccgtatttct ctaccggact
     2401 taccgtgtat cactttaacc ctatgtctac cttcaccgac ccggctaccg gactggatac
     2461 ggtgtatgca ctgcagcctc tgggaaccga aggtcagggt accagtgcat ttcctgaccg
     2521 caagccttat atgctcacgc aggtggccat accactggag ttcggtttta agtttactcc
     2581 agtcaggtat ctggccatcg ggttgacagg tggtgtaaag ctgaccttca cagactatct
     2641 ggatgacgta agtactacct acgttaaccc ggagatcctg atcaatgaaa atggaatggt
     2701 gtcctatctc ttatccaaca gaacctggga ggttactgaa aacggcgaac cacttgcact
     2761 gggaaacaag gccaccaggg gaggggcaga ctccaatgac tactatggat atctggcagt
     2821 taccttgtca tacctgttca taccggaata caagttcaat gtaagccgga aggcaggaat
     2881 tcactgcccc aaattctagt aattaccgca atattcgggt caaaaacctg ttttatcagt
     2941 tccatgcgac cctgtttgta aaggaacctg tatatttgtt tggtttaaag tgccatctat
     3001 gcgcatcaga aatctgcttt ttgcactctt actgacctcc gcaacgtcat ctatggcgca
     3061 aatgcccttg ttgagttggg tggagatcgg tgtgtttggg ggtgttgcca attattatgg
     3121 cgacctgaca caggactacg tggtttggga tgaatcgcac cctgcatatg gtggattcat
     3181 taagtataat ttcaactatc gctccggcct gaaactggat gttattcatg gaaccatctc
     3241 tgccgcagat gccaattctg accgtgcaga ccagctgctt cgcaacctga gctttacttc
     3301 tgacgtaaca gaagttgcgc tggtgtttga atacagcttt cccggaatgt ttcccaaaga
     3361 gttgcgaatg cctttttctc cctatgcaaa cgtgggtatt gcaggctttc attttaatcc
     3421 cagaaccttt ttccggggtg aatggtatga tctgcaaccc atcggtacag aaggacaggg
     3481 catcaatgaa ctggatttcc gcgaacctta cagcctgtat gaatttgcca tcccttttgg
     3541 aataggcgtt aaatgggcct tcagcgaacg atggaacctg gggctggaat atgcagccag
     3601 atggaccttt acagactacc tggatgatgt gagcagaact tatgttgaca gaaatctgct
     3661 gatcgaagag aatggtattc tggcttatca gctttccaac agaaccggcg aataccttct
     3721 ggcagacaat attgattttg gtaccaatga ttttcgtggt gatcctacag ataaagactg
     3781 gtacatgttc gtgggtctga cactgagcag gaatttcatc agtggcacag atgaaggttt
     3841 tatctcactg gataagccag gcctcggttg tttccaaccc aagtatgtga aaaagcatac
     3901 caagaagaac tatcgcagta ctttctgacc atgtccacct cctgtggtta acagattgac
     3961 ttcctgttca ggaagtttcc aagcatcttt atgccatgac accgggactg atattgctgg
     4021 tactgggatt gtatttctct ctgctgatgg tggtggctta tctcacctca cgcaaagcag
     4081 atcaatcatc cttctatatt ggcaaccgta actcacgatg gtatctggtt gctttcggaa
     4141 tgataggtgc cagcctcagt ggtgtgacct ttatgtctgt gccgggagct gtgggcgacg
     4201 gaggctttca ctattttcag atcgtactcg gataccttgc cggatacctg gtcattatca
     4261 gattgctgct gcctttgtac taccgcatgc agctgacttc catctacgaa tatctggagc
     4321 aacgtttcgg atcagttacc catcgctccg gcgcaggact ctttctgatc agccgggtac
     4381 tcggtgcatc cttccgttta tatctggtcg ccatcgttct gaataagttc attctggagc
     4441 ctttcggttt gccgttctgg gtagcggttg tggcaaccat cgtgctcatc tggatctatt
     4501 cctttcaggg cggtatcaag accatcgtct ggaccgatac cctgcagacc accttcatgc
     4561 tgctgtcagt ggttgtgacc ctgatctata tcaccggcca actggatctg aacgttagtg
     4621 aaacgatcgg aacagtgcgc aacagtgcct acagcagtat gttcgaatgg cactggcaac
     4681 cggctaattc attctataaa tatttcctga gtggaatgtt catcaccatc gtgatgaccg
     4741 gtcttgacca ggatatgatg cagaagaatc tgagttgcag gaatctggaa gagagccaga
     4801 agaacatgcg ctggatgagt atttctttgt tgccggtcaa cctgctcttc ctggtactgg
     4861 gggcactact gtatattttt ctggaacaaa gtggtacagg tcatgcagtg tttggtccgg
     4921 attgtcagat tgccatcggc gatgactgta tccggaaaga ttcactcttt gcttctcttt
     4981 ccattacggt attcccggtc tggatgggta tcgtatttct gcttggtctc acagccgcag
     5041 catactccag cgctgattca gctctgactg ctcttaccac cagtttttgc cttgatatac
     5101 ttcaacacaa agaacatact gtaccggtaa gattgcgtta tctggtgcat gccggattca
     5161 gtgtgctcct ggtattggtc attctgatat tccgcaacct gaataatgag tcagtgatca
     5221 atgagttgtt cacagtagcc ggatatactt atggtcccct gctgggattg tttgctttcg
     5281 gcatgtttac ccgcagacag gtaaaggatg cctgggttcc ggtggtctgt atcttatcgc
     5341 cggtggcatg ctattttcta aacaagtacg acacgcaatt attaggcggg tatcagttcg
     5401 gttttgaact gttgcttgta aatggagcac tcgcctggat gttattatat ttcatatcaa
     5461 aaaaacctga ggatgtcaga taagtaccgg aaaaagaaaa tgtataagac caaagactgg
     5521 acagagttta ccgctgagtc ttcctggcga atgttcaaga tcatggctga gttcgtagag
     5581 ggatttgaac gcatggataa gataggaccc tgtgtgtcca tctatgggtc tgcaagaaca
     5641 gatccggcta atccatatta cccgcttgca gagaagctgg ctgccagaat ggcgcaggag
     5701 ggttttggta tcattaccgg tggtggcccg ggtatcatgg aagctgccaa taaaggcgca
     5761 cattccatga atggcaaaag tgtaggactg aatattgaat tgccatttga acagtatgcg
     5821 aatccgtata ttgatcagga taaactcatc aacttcaact acttctttgt aaggaaggtc
     5881 atgctggtaa agtatgctca ggctttcatc tatatgccgg gtggatttgg cactctggat
     5941 gagttgtttg aagccattac actcattcag acccacaaaa tagaacgtgt gcctgtgatc
     6001 ctgatcgggg aagagtactg gaaggggtta ttgcagtgga tggataaaac agtgctgcac
     6061 gcagagcgaa atatcagccc tgaagacatg gatctttttt ttattaccga tgatgtggaa
     6121 gcggcagtga cccatgtgac tgagttttat gctacttcac acctgcgacc taatttctga
     6181 taatggcact gacttctatc actacactga aggtgggcgc taaggcaccc tcttttaaag
     6241 cactggatca ggatggaaga ccattcgact caaaagaaat gctgggaaag acctgggttt
     6301 tgtttttcta tccgaacgat atgacagcta cctgcaccat agaggtttgt gatcttcgtg
     6361 accattacaa aaagttgaaa caggcaggat tagagatcgt tggtgtttcc agaggagacg
     6421 tggcatccaa gaaaaagttt tccagcagaa ataaacttcc ttttcctttg ctggcagatg
     6481 aggatttgaa aatatcagga aaatataaag tttttggcga taaaccattc atgggaaaga
     6541 tcctggaatc catctacaga accacctttc tgatcggatc agatggtaag atacttcata
     6601 tcataaaaca agtaaaagca aaagaagcat cagctcagat cctcgaggcg gtaagatctc
     6661 tttagtcata tagttgcaat aatacaacgg tagctgaacc aatttccaaa aaaatgcttt
     6721 gtttgtgaaa attccacaaa tgaagagaac gattacattt tccatgctgt tttgtctggc
     6781 catgttattc gcatggcgtg gttatgtaca ggcaaaagaa gatggtgcgc caagcggtag
     6841 caccggagct ccgggcgatg atggaacctg tgctcaggtt gattgccata ccggcagcgc
     6901 tgtaccaaga agcggtttta tcagtacaga tatcccactt gcaggatatg tacccggcca
     6961 ggattattcc attgaagtaa gtgtcagctc tgagaacaga aatacatttg ggtttcagct
     7021 gacccctcag gatgcatcag gaattaaggt aggttccctc tccgtgaccg atgcagatcg
     7081 taccaagatc accggacttg gtaaatacat cacccataaa aaaaatggta ttgatggtac
     7141 agatggtatg atcaactgga actttacctg gacagctccg gaaagtccga ctgacctgac
     7201 tttttatgtg gcagttaata ttgctgatgg cgatggagat gcaaccggcg atagcatttt
     7261 tattgccaat ttgcctgtat ctgctgaccc gcttgcaacg gctattcatg aaatgaccat
     7321 tgctacaaaa gactggatga cagtaactcc gggtgatcaa ttgatgtttg cagactgggt
     7381 gttggctgaa caaccggtgg cagagatatg gaatctcagt ggtcaacacg ttagcagttt
     7441 cacccttgat gaaaagtctc attctgtggc catgctggac aaaggcatgt accttattac
     7501 gatacccaca ctttacggaa gactgacagg aaggtttatc agacagtaat tactgttctg
     7561 atcccttttc ttccgttact tctttgttgc ctgaggcttc ttcttcagca cgggctttga
     7621 tctcatcatc aatgatacca tgttcgttca gaatgtggta ccatttgatg atcttcttga
     7681 tatcactggt atatactttt tcctgatcgt gtgttggaag aatggccagg aaccagttgc
     7741 gcaatgcggc accatctgca ttggaatcag gagcaggatg ttcggccatt tttgcatgaa
     7801 tgtccagaaa aagatctgcc agttccacgt tatctgcatc tgtataaatg gagatgttgt
     7861 ccaatggtga gaacatattc tgtctgttgg atacgaactt cgtccaccct tcagccagag
     7921 aagtgacaat gagcccgttc actttctgac cctcaacctt gaacaatccc cccatgccgc
     7981 tgatggctac gatctctctg attcgcatat gctgttcttt tgcgcaaagg taagcaggaa
     8041 acgattcttc gtcacctgaa gttgtagatt tgccgaatgg cttttcaact tacatcgcag
     8101 tacaccccat caggagatca gcccggagct atagaccagc tggtgaatgc ggtgaatgaa
     8161 ggagaaaaag cacaggtgtt actgggggtg accggttccg gaaagacctt caccattgcc
     8221 aatgtcatcc agcaggttca gcgacctact cttatcctga gtcataataa aacgcttgtt
     8281 gcgcagctgt atggagaatt cagacaattc tttcctgaga atgcggtcga gtattttgtt
     8341 tcctactacg attattacca gcccgaagct tatatagcca ctactgatac ctacatagaa
     8401 aaggatctgt ctatcaatga cgaagtagag aagcttcgcc tgaaaacgac ctccagtctg
     8461 caaagcggaa ggcgagacgt tatcgtggtg gccaccgttt cctgtatcta tggtataggc
     8521 aacccggcag aattcaagga gagtattgtc cgggtcagtc agggtgatca ggtgacgagg
     8581 aattttttgc tgcacagact tgtggaagca ctgtatgccc gtacgctgac cgatttcaga
     8641 cggggaacat tccgggtgat cggcgatacg gtagaggtct atccggccta tgcagattat
     8701 tgctatcgga tcaccttctt cggtgatgag gtggaagaga tagagatatt tgatgctgat
     8761 accggcagga aaacagaaac ggccgaacac atcgccatct tccccgccaa cctctacatc
     8821 gctccgaaag aaatacttcc gcagatcatc catgagatcc atgaagagat ggacgaacag
     8881 gtggcttatt tcgaaagagc cggcaaatac atggaggcca aaagactgaa ggaacgggtc
     8941 aactttgaca tagaaatgat caaggaactg ggttactgtt ccggaatcga aaactattcc
     9001 aggtttttcg accgcagaaa acccggtagc cgcccgttct gcctgatcga ttatttcccg
     9061 gatgattttc tactggtcat tgatgaaagc cacgtgaccg ttccccagat cggagccatg
     9121 tatggc
//
LOCUS       DAOMAX010000088         9118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1073_length_9118_cov_4.310604, whole genome shotgun sequence.
ACCESSION   DAOMAX010000088 DAOMAX010000000
VERSION     DAOMAX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_9118_cov_4.310604"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..2825)
                     /locus_tag="PK742_06200"
     CDS             complement(243..2825)
                     /locus_tag="PK742_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQU76216.1"
                     /translation="MDAKFSPKVKEVISYSREEALRLGHDHIGTEHLLLGLIREGEGV
                     AMKVLSSLDVDVLLLRKSVEDAIRDKAPRQSFSAGSLPLTKQAEKVLKITVLEAKVLK
                     SETIGTEHLMLSILKNKDNIATQILGQFDVDYDIFRQELDFVKDGTAPDLPSNSAEDE
                     PYDDDEKGERQFSVPRKPGAAKSKTPVLDNFGRDVTRAAEEGRLDPIVGRQVEIERVS
                     QILSRRKKNNPILIGEPGVGKTAIVEGLALRIIQRKVSRVLFDKRIIMLDLAALVAGT
                     KYRGQFEERMKAIMNELEKNRDVILFIDEIHTIVGAGGATGSLDASNIFKPALARGEL
                     QCIGASTLDEYRQYIEKDGALDRRFQKVLVDPPTADETIEILSNIRPKYEEFHNVEYS
                     EEAIKACVTLSNRYISDRFLPDKAIDVMDEVGARVHLKNIHVPDEVVQLEGKIEEIKE
                     EKNKVVKSQRYEEAAKLRDTEKRLLEELETAKNKWEEQVKTEKYPVGEEDIAEVVAMM
                     TGIPTRRIAQSEGNKLVGMADELKQGVIGQDEAIVKITRAIQRNRVGLKDPKKPIGSF
                     IFLGPTGVGKTELAKVLSEFMFDSKDALIRIDMSEYMEKFSVSRLIGAPPGYVGYEEG
                     GQLTEKVRRKPYSVVLLDEIEKAHPDIFNILLQVLDDGQLTDGLGRKVDFKNTLIIMT
                     SNIGARQLKDFGQGVGFSTGAKQAAAEDHSKGVIESALKRTFSPEFLNRIDDVVIFNS
                     LGKEDIFRIIDITLREVVGRLETLGYGLKLEDSAKDFLAEKGYDPQFGARPLHRAIQK
                     YLEDPLAEQILAGTFKTGDTIKVTFNEQEGRLEIAPASTGKKKAKKEEESEEKPEEQN
                     EEKD"
     gene            3052..3423
                     /locus_tag="PK742_06205"
     CDS             3052..3423
                     /locus_tag="PK742_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQU76217.1"
                     /translation="MKFTVDKKERYCIFRPDEDKLNSLLSPDLKSELVILNQEGYRNI
                     ICDLSPVTFIDSSGLSSLLTGHRLCQESKGTFVLMGCNEAVQKLIKISQLDSILNVVP
                     TESEATDYILMEEVQREIEGE"
     gene            3427..4371
                     /locus_tag="PK742_06210"
     CDS             3427..4371
                     /locus_tag="PK742_06210"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HQU76218.1"
                     /translation="MTFQITILGSNSAVPAHGRNPSAQVVEISKKPYLIDCGEGTQMR
                     FSQYGIKSSRIEQIFISHLHGDHYFGLIGLISSYHLLRRNKPLDIFSPAGLEEIIRVQ
                     LEAGQTELCYPLHFHIVDTERHQMIFENRQMEVWSLPLNHRIPCSGFLFREKKKERRI
                     NKELFEAMQLPHHLAPRLKAGEDVQDPNTGEFLSNKKLTLPPNHERSYAYCSDTAYYE
                     PLIDLIKDADILYHDCTFDKAGTDRAVQTYHGTTEHAATIAQKAGVRRLLIGHFSAKY
                     EDLSPLLEEAREIFPETELALEGEIFQVEAAPVLFNKG"
     gene            complement(4354..4974)
                     /locus_tag="PK742_06215"
     CDS             complement(4354..4974)
                     /locus_tag="PK742_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76219.1"
                     /translation="MTHTRNLLWAACLLFPLSLMAQEPALNRVQAMPDADDTELFIKE
                     FHRVMNLIANDFPNDFKNIKGPVIMDDGEATSNFSTSAALPEASEAYITTRLEADFST
                     VNTVYVAVFFDGIDGETADVIYSNLVWLINNCSFECCNMVYDELDDSADSGAKTTFYL
                     PYDSNNTEAYENMLFEVRLFKSFDLTDDLELIDTWMVVLNVSTFVE"
     gene            5063..5701
                     /locus_tag="PK742_06220"
     CDS             5063..5701
                     /locus_tag="PK742_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HQU76220.1"
                     /translation="MRIRSLQTILPVILLLFTSGTGDEGIRIGKQTWMNRNLEVEQFS
                     NGTRIKQAKTEDEWLDAGAKGEPVWCYYEFDDKLGKPYGKLYNWYAVSHPSGLAPSGW
                     RIPEVDDYLKLMEEFGGKDSCALVLKNTDGWSGRKKGNGSNASGFSALPGGMVDDAGW
                     FGLEGYFGGWWTASTDSTGKPVMVGLDFAHTGATFTSEDKSFGYAVRCIQGK"
     gene            complement(5705..7060)
                     /locus_tag="PK742_06225"
     CDS             complement(5705..7060)
                     /locus_tag="PK742_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQU76221.1"
                     /translation="MANILLVDDEKSIRKTLREILEYEGYAVDEAQDGAEGLRMIQDG
                     DYDIVLCDIKMPKMDGMEVLAKATKSNPDVPIIMISGHGTLETAVDAVKNGAYDYVSK
                     PPDLNRLLITIRNALDKSNLITETKVLKRKVTKTREIIGESPGISKIKDTIERVAPTD
                     ARVLITGDNGTGKELVARWIHERSNRATGPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AVKQRIGKFEQANGGTLFLDEIGDMSLSAQAKVLRALQENKITRVGGDKEIPVDVRVV
                     AATNKDLLKEIDAGNFRMDLYHRLSVILIHVPSLNEREDDIPLLAERFVKEICEDYGM
                     PRKAITPKAMLELQAITWTGNIRELRNVIERLVILSDQKITEEEVQSYAQTPKSGGNP
                     KDLFDRFEKFQDFKEYIEKEFILHRLQKNSWNVSKTAEELDIQRSHLYNKIEKYDLKR
                     E"
     gene            complement(7090..7842)
                     /locus_tag="PK742_06230"
     CDS             complement(7090..7842)
                     /locus_tag="PK742_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HQU76222.1"
                     /translation="MIRTSKKILLTNLILGCLTTIAAAQDTTFTLLDPTTGLTAYQLE
                     NKDATSEEGYLLNGKKEGLVRTFFANGVLQTATEYHEDIPNGWYLECEKNGVVLKEEH
                     YKMGVIDGEVRTYTTKQGVRIVQAIYHYRDGKLHGTCADYSDMGKMSALATYSYGIKE
                     GPSRWYYTNGKPAMEQHYVNGELYGTTTSWGPDGKVTQYGNYKHNQKDGEWKEYYTGG
                     QIKSEGSYVADEKTGTWKYYDEEGNLSNSETY"
     gene            complement(8017..8387)
                     /gene="rnpB"
                     /locus_tag="PK742_06235"
     ncRNA           complement(8017..8387)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK742_06235"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(8402..>9118)
                     /locus_tag="PK742_06240"
     CDS             complement(8402..>9118)
                     /locus_tag="PK742_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQU76223.1"
                     /translation="AAGEIERYLLVLINRTDADLRIHYEFLLQGSVYYSYRKELADGA
                     DTDLHHFRTDQLNDLPAFRITCWPLKSSEQTAHEAVREVRLKAKQFFSPVYAFGNDGA
                     LLMPFWKSLPGKMEKPVFQTPADDFDWEEQDTPQVHEVLEKASMPDFIDLHAEKLDQA
                     WELLDKQEILQMQLNHCRNFVERAIRHKLHKVYVIHGLGKGILRKEIERLLDEYPSVT
                     SYSNQYNPRFGHGATEVILE"
BASE COUNT         2137 a         2268 c         2135 g         2578 t
ORIGIN      
        1 tggacggcaa aaaaagacga cccgggtttc acctgaggtc gtttttttat tggcggacac
       61 acgaatgcga agcatacgca gatggggtta gttcacccca cttttatgga tcgtgtcaac
      121 catccggtca tcccgacaat cacatctgac tttcgaagaa atttgcggac gacaggaaag
      181 atcaactgtt aaaaaggccc gggaaaaccc gggccgtgtc tgttcatcta atggctgaag
      241 gatcagtctt tctcttcatt ctgctcttcc ggcttctctt cagattcttc ttccttcttt
      301 gcctttttct tgcctgttga agcgggagca atttcgagcc tgccttcctg ttcgttgaac
      361 gttaccttaa tggtatcgcc tgttttaaag gtgcctgcca ggatctgttc tgcgagcggg
      421 tcttccagat acttctgaat ggcacggtgc aaaggtcttg caccgaactg aggatcatat
      481 cctttttcag ccaggaagtc tttggcagaa tcttccagtt tcagaccata accaagagtt
      541 tccagtcttc caaccacttc acgcagggtg atgtcaatga tcctgaaaat atcttcctta
      601 cccagtgaat taaagatgac cacatcatct atcctgttca ggaattcagg agagaaagtg
      661 cgcttcaggg cactttctat cacaccttta ctatggtctt cagcggctgc ctgtttggcg
      721 ccggtactga agcccacgcc ctgaccgaaa tctttcagct gacgggcacc aatgttggag
      781 gtcatgatga tgagtgtgtt cttgaagtca accttgcgtc cgagcccgtc tgtaagctgg
      841 ccatcatcaa gaacctgcaa cagaatattg aaaatgtcag ggtgagcttt ttcaatttca
      901 tcgagcagca ctacactgta tggtttgcgt ctgacctttt cggtcagctg cccgccttct
      961 tcatacccaa cataacccgg aggcgcaccg atcagtctgc tcacactgaa tttttccatg
     1021 tactcactca tatctattcg aatgagagcg tctttggaat caaacatgaa ctcactgagt
     1081 accttggcca gttctgtttt gcccacaccg gtaggcccca ggaatatgaa tgagccaatg
     1141 ggttttttag gatctttcag tcccacccgg ttgcgctgga tagccctggt gatcttgacg
     1201 atggcttcgt cctgcccgat gactccctgt ttcaattcat cggccatacc taccagtttg
     1261 ttgccttcac tttgggcaat acgtcttgta ggaatgccgg tcatcatggc cactacttct
     1321 gcaatatctt cttccccgac agggtatttt tctgtcttga cctgttcttc ccatttattt
     1381 ttggcggtct ccagttcttc caacaggcgt ttttctgtat cgcgaagttt ggcagcctct
     1441 tcatatcgct ggctcttgac caccttattc ttctcttcct tgatctcttc gatctttcct
     1501 tccagctgca ctacctcatc aggaacatgg atgttcttca ggtgcacacg agcgcctact
     1561 tcatccatta catcaatggc cttatcgggc aggaagcgat cgctgatgta tcggttactg
     1621 agtgtcacac aggctttgat ggcctcttcg ctgtattcaa cattatgaaa ttcttcatat
     1681 ttaggcctga tgttgctgag aatctctatg gtttcatcgg cagttggcgg atctaccaga
     1741 accttctgaa agcgtctgtc aagtgctccg tccttttcga tgtattgtct gtattcatcc
     1801 agggtggaag ctccgatgca ttgcagttca cctctggcca gagctggttt gaaaatattg
     1861 gaagcatcca gtgaaccggt agccccgcca gctccaacga tggtatgtat ctcgtcaatg
     1921 aagaggatga cgtcgcggtt cttttccagc tcattcatga tggctttcat gcgttcttca
     1981 aactggcccc tgtatttggt acctgccacc agcgctgcga ggtccagcat aatgatgcgt
     2041 ttgtcaaaca gcacccggct aaccttacgc tggatgatgc gcaatgcaag gccttctacg
     2101 atggcggtct ttccgacacc gggttcacca atcaggatcg ggttattctt tttgcgccga
     2161 ctgaggatct ggctgacacg ttcaatttcc acctgtctgc ctacaatggg atccagtctg
     2221 ccttcttcgg cggcacgggt cacatcacgg ccaaagttgt ccagtacggg tgttttgctt
     2281 ttggcggcac ccggttttct gggaacgctg aactgacgtt cgcctttttc atcatcatca
     2341 taaggttcat cttctgctga gttggaaggc aggtcaggag cagtaccgtc ttttacgaaa
     2401 tccagttcct ggcggaagat gtcataatcc acgtcaaatt gtccgagtat ctgagtggcg
     2461 atattgtctt tattcttcaa aatggaaagc atcaggtgct ccgtgccgat ggtttcgctc
     2521 ttcagcactt tagcctccag cacggtgatc ttgagaactt tttcagcctg tttggtaagt
     2581 ggcaggcttc cggcgctgaa tgactgacgc ggagctttat cacgaatggc atcttctact
     2641 gatttgcgca gcagcagcac atctacatcc agactactga gcactttcat tgccacacct
     2701 tcgccttcgc ggatcaggcc taacagcagg tgttctgtgc cgatgtggtc atgccccagc
     2761 cgcagggctt cttccctgct gtaggatata acctctttta ctttcggaga aaattttgca
     2821 tccatatatg tgtgtacgtt caaaaattgc accaataagg agaaacatga cagaattaca
     2881 acggcacctt cgggtggatt gttctgtaac gcagtttaag agccgttttt atgacagaaa
     2941 aaagattccc tgccggaggc tgcgctcttc tcacaaattt gtgaaataat ctccattacc
     3001 tgttatccac aagtgcctaa aagtttttat cattgttcac aaatcaagta catgaaattc
     3061 actgttgata aaaaggaaag atactgcatt ttcaggcccg atgaagacaa attgaacagc
     3121 ctgctcagcc cggatcttaa aagcgagctg gtcattctga accaggaggg atacagaaac
     3181 atcatctgcg atctcagccc ggttactttc attgacagtt ccggactcag cagtttactt
     3241 accggacatc gtctttgtca ggagtcaaaa ggcacttttg tgctgatggg atgcaacgaa
     3301 gcggtacaaa aactcatcaa aatttcacag ctggattcca ttctgaatgt agtacctaca
     3361 gaaagcgagg ctactgacta tatcctgatg gaggaagtac agcgtgaaat tgaaggtgag
     3421 tagcctatga cctttcagat caccattctg ggcagcaatt cagccgttcc tgcacacggc
     3481 agaaacccta gcgcccaggt cgtggagatc agcaagaaac cctatctgat agattgcgga
     3541 gagggcaccc agatgcgttt ctcgcagtat ggtataaagt ccagccgcat cgaacagatc
     3601 ttcatcagcc atttacatgg cgatcattac tttggtctga tcggtctcat ttcttcctat
     3661 catttgttaa gacgtaataa gccgctggat attttttcgc cggcagggct ggaagagatc
     3721 attcgtgtgc aactggaggc gggacaaacg gaactttgtt atccgttgca tttccatatt
     3781 gtagatactg aacggcatca gatgatcttt gaaaacaggc aaatggaggt ctggtcactt
     3841 ccgctcaatc accggatacc ctgcagcgga tttctgttca gagagaaaaa gaaagagcgc
     3901 cgcatcaaca aggaactgtt cgaagcgatg cagttgcctc atcacctggc accccggctg
     3961 aaagcaggtg aagatgttca ggaccccaac acaggcgagt tcctgtcaaa caaaaagcta
     4021 acgcttccgc caaatcatga acgcagttac gcctactgca gtgataccgc ctactacgag
     4081 cccctaattg acctgataaa agatgcagat attctttacc atgactgcac atttgacaaa
     4141 gccggtactg accgtgctgt tcaaacctat catggtacca ctgagcatgc tgccacgatt
     4201 gcacaaaaag ccggagtgag acgtctgctc attggacact tcagtgccaa gtacgaagat
     4261 ctgtcgccct tgctggaaga agccagagag atcttccctg aaaccgagct tgctctggaa
     4321 ggggagattt ttcaggtaga agcagcccct gtcttattca acaaaggttg agacattcaa
     4381 cacgaccatc caggtgtcta tcaattcaag gtcgtcggtc aggtcaaaag atttgaataa
     4441 tctcacttcg aacaacatgt tttcgtatgc ttctgtattg tttgaatcat agggaagata
     4501 gaaggttgtt ttggctccgc tgtcagctga gtcatccagt tcatcataca ccatattaca
     4561 gcattcaaag ctgcagttat tgatgagcca aaccaggtta ctataaataa catcagcagt
     4621 ttcaccatct attccgtcaa agaaaacggc tacatacacc gtattgacgg tactgaaatc
     4681 tgcttccagc cgggtggtga tatatgcctc agatgcttca ggtaaagcag cgctggtact
     4741 gaaattactc gtagcttctc cgtcatccat gatgaccggg cctttgatgt tcttaaaatc
     4801 attcggaaaa tcattggcaa tgaggttcat gaccctgtga aattctttga tgaacagttc
     4861 tgtatcatca gcatcaggca tagcctgtac cctgttcagg gcaggctcct gggccatcag
     4921 agagagtggg aataacaggc aggcagccca cagtagattg cgtgtatgtg tcattggttc
     4981 aaatttctgc atcaaagtaa acaaaatcaa ggcaataaga cgttgtgaat gaagctcctt
     5041 acctttaaat aaattccatc ttatgcgcat tcgcagtttg cagacaatac ttcctgttat
     5101 tttattgttg tttacttccg gcaccggaga tgaaggcatc aggatcggga aacagacctg
     5161 gatgaaccgg aatctggaag tggagcaatt cagcaacggc acccggatca aacaggcaaa
     5221 gacggaagat gaatggctgg acgcaggtgc caaaggcgaa ccagtttggt gttattatga
     5281 gttcgatgat aaacttggta agccatacgg caagctgtat aactggtatg cggtgagtca
     5341 cccctccgga ctggcacctt caggatggcg tattccggaa gtggacgatt acctgaagtt
     5401 aatggaagaa tttggcggaa aggacagctg tgcactggtg ttaaagaaca ctgatggctg
     5461 gagcggaagg aagaaaggca acggcagcaa tgcgtccggt ttcagtgcat tgcctggtgg
     5521 catggtggat gatgcaggct ggttcggact ggaggggtat tttggcggat ggtggactgc
     5581 cagtactgac tcaaccggaa agccggtcat ggtggggctt gactttgccc atacgggagc
     5641 caccttcacc tctgaagaca agagtttcgg ttatgcggtc aggtgcatac agggtaagta
     5701 gtgatcattc tcttttcaga tcgtacttct cgatcttatt gtagagatgt gatctttgga
     5761 tatccagttc ttctgctgtc ttggaaacat tccagctgtt cttttgaagc cggtgcagga
     5821 taaattcctt ttcaatgtac tctttgaaat cctggaattt ctcaaagcga tcgaacagat
     5881 ctttcgggtt accgccactt ttaggggttt gggcataaga ctgcacttct tcttcagtga
     5941 tcttctgatc actcaggatg accagacgtt ctatgacgtt gcgcagttca cggatatttc
     6001 ctgtccaggt aatagcctgc agttccagca tggctttagg tgtgatagcc tttctgggca
     6061 taccgtaatc ttcgcagatc tctttgacga aacgttcggc caggagtgga atatcatctt
     6121 ctctctcatt cagagaaggc acatggatga ggatgacact caggcggtgg tagaggtcca
     6181 tccggaaatt accggcgtcg atctctttca gcaggtcttt attagtagcg gccaccaccc
     6241 gtacatcaac agggatctct ttatcgccgc caacccgtgt gatcttgttt tcctggaggg
     6301 cacgaagcac tttggcctgt gcgctgaggc tcatgtcgcc gatctcatcc agaaacaatg
     6361 tacccccatt ggcctgttca aacttaccga tgcgctgctt gacggcagaa gtaaaggcac
     6421 ctttttcgtg gccgaacaat tcgctttcga tcagttcact gggaatggcc gcacagttga
     6481 cctcgatcag cgggcctgtg gcacgattgc ttcgttcatg gatccatcgg gcaaccagtt
     6541 ctttaccggt accgttatcg ccggtaatca gaacccgtgc atcggttgga gccacccttt
     6601 cgatcgtatc cttgatctta ctgatacccg gcgactcgcc gatgatctct ctggtcttgg
     6661 tcaccttgcg cttcagcacc ttggtctctg tgatcaggtt gcttttatct aaggcattgc
     6721 gaatggtgat gagcagcctg ttcagatcag gtggcttgga aacataatca taggctccat
     6781 tcttgaccgc atcaacagcg gtttcaaggg taccatggcc ggatatcatg atgatgggta
     6841 catcaggatt tgacttggtt gctttagcca gcacttccat tccgtccatt ttcggcattt
     6901 tgatatcgca tagcacgata tcataatctc cgtcctggat catgcggagg ccttcggcac
     6961 catcctgggc ttcatctacg gcataacctt cgtattccaa aatctcccgc agggtcttgc
     7021 ggatgctctt ttcatcgtca accagtaaga tattcgccat ggctgtaaaa gtacgtaatt
     7081 gacaagagtt tagtatgtct cagagttgct cagattccct tcttcatcgt agtatttcca
     7141 ggttccggtt ttttcatcgg ccacatagct tccctctgat tttatctgac caccggtgta
     7201 gtattccttc cattctccgt ccttctggtt gtgcttgtaa ttcccgtatt gggtcacttt
     7261 gccatccggt ccccaggagg ttgtggttcc atacagctcg ccattgacat aatgttgttc
     7321 catagccggt ttgccattgg tataatacca tctgctgggg ccttctttga taccatatga
     7381 ataagttgcc agggctgaca tttttcccat atcagaataa tctgcacatg ttccatgcag
     7441 ctttccgtct cgatagtgat aaatagcctg gacgatccga acaccttgtt tggtagtata
     7501 ggttcttact tctccatcta tcactcccat tttatagtgt tcttctttca gcacgacgcc
     7561 attcttctcg cattccaggt accaaccgtt cggtatatct tcatgatatt ctgtggcagt
     7621 ttgcagtacg ccgttggcaa agaatgtgcg cacgaggcct tctttttttc cattcaacag
     7681 atagccctct tcgctggtgg catctttgtt ttccagctga taggcagtca gcccggtagt
     7741 gggatccagg agtgtaaaag tggtatcctg tgcagcagcg atcgtagtca ggcaacctaa
     7801 gataagatta gtcagcagga tctttttgga tgttctgatc atggtctttt tgtttccttt
     7861 ctgtttgttc aatttgttca ccacggcagc attcatcaat caccgtgtgg caaacggaac
     7921 actggccgtg tccgtgcacc cggatcaccc tgacgggatt tccgcagtac ggacagataa
     7981 tataatctct gagggaatgc attcccatat tctaaaaaga tctgcaggct cataagccgg
     8041 attctgtttg agtcctgtca tttatctggg catacattca catgcatgct cttgctgcct
     8101 acccgccgga ggttcagacg agcagccttc tctccgcgaa ggatactccg gcttatttgg
     8161 cattgtaacc cgcaaggttt acccatgcac gctgttacca gcatgcatcg tgggctctta
     8221 ccccacgttt tcaccttgac cccggacaaa ccggggcagt tattttctgt ggcactttct
     8281 cttgcacgtt gccgtacagc cacccgttag gtggtgcggt actctgtgtt atccggactt
     8341 tcctcatcac gccgtaaacg acgagacgcg acaggccggc ctgcagatat gttgaagaac
     8401 gttattccag aatgacctcg gtggcaccat gtccgaaccg cggattgtac tggttggaat
     8461 acgaagttac tgaaggatat tcatccagca agcgttcgat ctccttcctc agaatgccct
     8521 ttcccaggcc atgaatgaca tagaccttat ggagtttgtg ccggatggcc ctctccacaa
     8581 aattccggca atggttcagc tgcatctgca ggatctcctg cttgtccagc aattcccatg
     8641 cctgatccag cttttcagca tgtagatcta tgaagtcagg catggaagct ttctccaata
     8701 cttcatgtac ctggggtgta tcctgttctt cccagtcgaa gtcatcggcc ggagtttgga
     8761 ataccggttt ttccatcttc cccggcagtg acttccagaa tggcatcagg agtgccccat
     8821 cattgccaaa ggcatagacc ggactgaaga actgttttgc tttaagtctc acttcacgca
     8881 ccgcctcatg agctgtttgt tctgaacttt tcaatggcca gcaggtgatg cggaaagccg
     8941 gcaggtcatt cagctggtct gtgcgaaaat ggtgcagatc ggtgtcggct ccgtcggcca
     9001 gttcctttct gtagctgtaa tacacactgc cctgcaggag aaattcatag tggatgcgaa
     9061 ggtctgcatc tgtgcggttg atcaacacca gcaggtatct ttcaatctct ccggctgc
//
LOCUS       DAOMAX010000089         9057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1084_length_9057_cov_3.992557, whole genome shotgun sequence.
ACCESSION   DAOMAX010000089 DAOMAX010000000
VERSION     DAOMAX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_9057_cov_3.992557"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..3333
                     /locus_tag="PK742_06245"
     CDS             193..3333
                     /locus_tag="PK742_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU76224.1"
                     /translation="MGVLCRSVLLTWLILAVNHNAFTQFHEVRFRHIGIEDGLSQSSV
                     NTIYQDSRGFIWIGTQDGLNRFDGTDFTIYRTGLNDHAICGNYIYDILEREDAEMWVA
                     TFGGGLIRIDQRTQQIDCFSQSEGNLPSNRIFSIAEDDQQILWLGSNEGLIRFDPKEK
                     TSEVLFHSRMNFIGSVEWIQDRLWIGGDSGLIVLDPVTRKSDENWIDLVGHTGTVSTI
                     AASGDTVMLACQEGLFCINTRTGIKSEISFPDHLPKGVIQSVCRIHNGNWLIATGNGL
                     FEWHPVRGIINTYYSNVSDPASLSNNNVIAVCQSEDQVVWVGTRNGLSVIENPEPAIR
                     LVRSIPGESGLQSRQVNAFAAYNDTLVWAGTSNGLHLIDMEQELVTPWNSSSSNAYMS
                     FPVEILSLYRDEEGVIWVGTKQDGVYRLTGSAVQPVLQKISVPGAERLSVHCIRKDPT
                     GRLWLGTSAKGLWRVDPDGSAPVIYSYQEDGTGLSHTYVFCLEADTRDRMWIGTPTGG
                     VNVLDTRSEKIWHIQNDPEEPGSLSNDIILSFYTDDRGYLWVGTVDGLNRTELPLTEI
                     TADLLDRGKVRFRKYGRNEGLPNEVVYGMLDDGAGSLWMSTNRGIAVFHMADEKVSAV
                     ITPADGLQNNEFNQNAFYLTSNGTMFFGGVEGMNFFEPGKVFSGKDALQVVLTNVALF
                     NETVPVGGNGLYGLHLENDIGFMDELELSYKHDMVTFHYAALSFKRPEQTVYRYKLEG
                     FDKKWVYADDGRSATYTNLKPGKYSFLVEAAGPDLNWSGTSIKVMVGTPPWLTWYATL
                     FYLLLAAGLIYTYTRLRIRAETRKLLQQQEVANARIEERELYRKKSAADFHDEAGKFI
                     TKINLFAEMAGKEAAQEEKLSGFIQKIRENTLELGTNMRDFLWSMDVRMDNLYDLVDR
                     MRDAGELMLTETGIMFRAEGLDPEFKNVPLSQEMRRVCLQLFKEAFLNTARHSGATKA
                     VLKVTLSGQQLQVLYQDNGTGIVLNEAAKKGHYGLKLMQERAEKIGGSLQIESPEKGG
                     TLVTLDVKIPQMGDTE"
     gene            3361..3993
                     /locus_tag="PK742_06250"
     CDS             3361..3993
                     /locus_tag="PK742_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU76225.1"
                     /translation="MDIQESIRVAIVEDEQEIRELLEQIIDLSPGFSCKRTFSSCEDA
                     LPGLIADPPDVVLMDINLPGMDGISGTGKLTEQIPELNIIMLTIQDDPDLIYQSLCAG
                     ATGYLLKDTPPVKLLQAIEEVRQGGSPMSPSIARKVTMSFRPAVNYDLSDREKEILTR
                     LTQGENYQVIAAELFISGHTVRTHIRNIYRKLQVNTRGEAVSTALRNKIV"
     gene            4078..4620
                     /locus_tag="PK742_06255"
     CDS             4078..4620
                     /locus_tag="PK742_06255"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HQU76226.1"
                     /translation="MKTSGLLTLLFAFVALAAACNKEQEPDPNNNNNNNNNGGNNPPT
                     ETMLSCKTDGADWQSDTTNVVAVMVEVAGTKTLTISGEAADGSSLVLSISLWTGVEGS
                     FPTSLMSFTNYVAMAYTDTSDEAFSAPNINNAAATGTLTITYWDEEKVSGSFSFTGGQ
                     DDTSDTMTISEGSFSVTQVN"
     gene            4626..5642
                     /locus_tag="PK742_06260"
     CDS             4626..5642
                     /locus_tag="PK742_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76227.1"
                     /translation="MKRVHIIHLVLWALVPTAGIMAQPVIGLSNMPQVGDSVIIALSN
                     QPVAPGTAGADQTWDMTWISAAEEQYFVYEDPAVTPWADLFDDATICGRSWEDYYSYY
                     HIDATGVAAVGYAGVIGLSDTFTMDYSDSELFIPLDYSFGDSHADDFAGTSTAAGFSL
                     PFTGDVDFEADGYGTLILPTGTYENVVRYHFFRTQEITFGTTTTTTKEQWAWVSSDYR
                     FWLLLMEENFDGFSTTPLIWYDKAPLPAVPVAIGSAENYSVRLFPNPVSAQQSLSFEW
                     NEQSNAVLTLTDLHGRKVWQRNMELKPGMQSLSFDGQVDAGCYLLQIATEKADHVTRL
                     IVSE"
     gene            5746..7791
                     /locus_tag="PK742_06265"
     CDS             5746..7791
                     /locus_tag="PK742_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76228.1"
                     /translation="MIQKLSSLFVWLLAGLIQTASAQFNHLWSYSHYSNNNDEIKAIA
                     AHDSGTVVTAGSFINTCIFDGDGPSLNLTSAGGKDVSLASYQADGTLNFVIGFGGSSD
                     DVAADVVLDADGNIYVTGSFQNSMDVDPGAGEFILEASGLNDVFLIKYNALGELVWAH
                     RFGEDFTDDGKELVLGDDGFLYMSGGYRIEMKFAGVADSITLSNPGFTTDVFLAKFNL
                     DGEAVWARSFGGGGSDFVDGLAYWNDMLFLTGDFTTTIDLDASAAVDNHSSNGLNDVF
                     LCAYDTDGDYLWGHTFGGSSADFGHDLATDNDGNIYMTGNFNNTINFSSTGGSLELTS
                     ALQSDMFLAAYTAFGDDLWAIRAGGSDAEEGYAVAVSASGPVVTGTFGSTVDFDPGPG
                     TWEQTSAGFYDIYLVQYDFDGALIEAGNMGSPYSDYGHVLTMAPNGDILTGGRMQSPL
                     DIDPGPGEILLYGNTGFDGYIARYNTCISYFLEESATICAGETYDFHGTVLSEAGEYV
                     ASFTTVDGCDSTFELSLVIHDIDITITSDDTVLNAVYSPDYSYQWLDCNDGMTPVEGA
                     TEAVFSATESGSYAVEITWGPCSEISACVSVDIEEPVGFSEELHADWLLWNGLNNWII
                     SSDQPAQVQVFDMQGQAVYNSTINGTTELSTGQLAAGWYILKYSTDLYQDSRKILVY"
     gene            complement(7794..8924)
                     /locus_tag="PK742_06270"
     CDS             complement(7794..8924)
                     /locus_tag="PK742_06270"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HQU76229.1"
                     /translation="MKFLSCILLLASVSVGAQTTVIDSIHSGGQSRQYYLYVPAAYDG
                     SEPRPLVLNLHGYGSYAFQQIFYGEFRPIADTANFLILLPDGTQDVLGFQHWNTFNAF
                     GTGVDDVAFISDLLDSISAEYNIDQNRIYSTGMSNGGFMSYELACQLSDRIAAIASVT
                     GTMDTDHFDYCSPSHPTPVMEIHGTADATVPYAGSGDFLPIDELLDYWISYNNCNTTP
                     EFTEIPDTDPGDGSTVEHYIWSGGDNGVSVEHFKVINGGHTWPGTILLLPASGSTNKD
                     INASAEIWRFFSQYSLELLTGLDESDAHPEELLVYPNPGNGLFVLEFPGKNSPIPVLV
                     KDLSGRLVMTGNSADEQMDIRSLPAGIYMLTAGNAGTIYIKQ"
BASE COUNT         2409 a         2053 c         2261 g         2334 t
ORIGIN      
        1 gaagtggaaa ctgagttcgc ttttctgcat gctgttgctg cccgtgatga atggttgatg
       61 gtagagttgc ttgacagtga tctgaataag atcagcacgg cctggctaaa atggcgaaac
      121 agtgaaagaa tgctccttac ctggtcgctt tttatgtagg tatttgtcca ccgcaatgcc
      181 tattttagag ttgtgggtgt tctctgcaga tctgtcctcc tgacctggct gatactggct
      241 gtcaatcaca acgctttcac gcagtttcat gaagtgcgct tcaggcatat tggtattgaa
      301 gatggcctgt ctcaaagttc tgtcaatacc atctaccagg atagcagagg ttttatttgg
      361 ataggcacac aggatggcct gaatcgtttt gatggtaccg attttaccat atatcgcaca
      421 gggctgaatg accacgctat ttgcggaaac tatatctatg acattctgga aagagaagac
      481 gcagaaatgt gggtggcaac tttcggcggg gggctcatca ggatagatca gcgaacgcag
      541 cagatcgatt gcttcagcca gtctgagggt aacctgccca gtaacagaat cttttcaata
      601 gcggaagatg atcagcagat tctttggctg ggaagtaatg aaggactgat caggtttgat
      661 cccaaagaaa aaacttcaga ggtcttgttt cacagccgga tgaatttcat tggaagtgta
      721 gaatggatcc aagacagact ttggatcggc ggcgattccg gtttgatcgt actggatccg
      781 gtaactcgaa aaagcgatga gaactggata gacctggtgg gtcataccgg aactgtcagt
      841 accattgccg ccagtggcga tacggtcatg ctggcttgtc aggaaggact gttctgtatc
      901 aataccagaa ccggaatcaa gtctgagatc agctttccgg atcacctgcc caaaggagtc
      961 attcaatctg tctgcagaat ccacaacggg aactggttga tagccaccgg taacgggctt
     1021 tttgaatggc accctgtcag aggaattatt aatacgtatt atagtaatgt gtcagaccct
     1081 gccagcctca gcaacaacaa tgttatagcc gtctgtcagt ctgaagacca ggttgtgtgg
     1141 gtgggcacca gaaacggatt atcagtcatt gaaaatccgg agccggctat aagactcgtc
     1201 agaagtattc ccggcgagtc aggcctgcaa tcaagacagg ttaatgcctt tgcagcttat
     1261 aatgataccc ttgtctgggc cggaactagc aacgggcttc atctgattga catggaacag
     1321 gaactggtga ctccgtggaa tagcagctct tcaaatgcct atatgtcttt tcctgttgag
     1381 atactgagtt tgtatcgcga tgaggagggt gtaatatggg taggaaccaa acaggacggt
     1441 gtgtaccgcc tgactggcag tgctgttcag ccggtgctgc aaaagatctc tgttccggga
     1501 gctgagcggt tatctgttca ctgcatacgg aaagatccaa caggcaggct ctggctggga
     1561 acatctgcaa aaggtctctg gcgtgtagat ccggatggat cagctcctgt catttattct
     1621 tatcaggaag atggcacagg gcttagtcat acttatgtat tctgtctgga agctgatacc
     1681 agagatcgga tgtggatagg cacccccacg ggcggggtga atgtacttga tacaagatca
     1741 gaaaagatct ggcatattca gaatgatccg gaagaacccg ggtctctgag taatgacatc
     1801 atattatctt tctatacaga tgatcggggc tatttgtggg tgggaaccgt tgatggattg
     1861 aaccgcaccg aactcccgct tacagaaata actgccgacc tgcttgacag gggaaaagtg
     1921 cgttttagaa aatatggaag aaacgaaggt ttacccaatg aagtggttta tggtatgctg
     1981 gatgatggtg caggctctct ctggatgtca acaaatcggg gcattgccgt ctttcatatg
     2041 gccgatgaaa aagtgtctgc tgttatcacc ccggccgatg gtttacagaa caacgaattc
     2101 aatcagaacg ctttttatct tacttcaaat ggcactatgt tctttggtgg tgtggaagga
     2161 atgaattttt ttgaacccgg gaaggtattt tcaggtaagg atgccttaca ggtggttctg
     2221 accaatgtgg cattgttcaa tgaaacagtt cctgtgggtg gaaatggact gtatggcctc
     2281 cacctggaaa atgatattgg ttttatggat gagctggaac tttcctacaa acatgacatg
     2341 gtgacctttc actatgcggc tttgtcattc aaaagacctg aacaaacagt gtaccgatac
     2401 aagctggaag gttttgacaa aaaatgggtg tatgcagatg atggtcgttc agctacttat
     2461 acaaacctca aacccgggaa gtatagtttt cttgtagaag cagcaggccc tgatctgaac
     2521 tggtcaggca cctctataaa agtcatggtt ggcactccgc cctggctcac ctggtatgcc
     2581 actttgtttt atttgctgct agctgcagga ctgatctata cctatacaag attgcgcatt
     2641 cgggccgaaa ccagaaaact gctgcaacaa caagaagtgg ccaatgcacg gatagaagag
     2701 cgtgaattat accgaaaaaa aagtgcagct gattttcatg atgaagccgg gaaattcatt
     2761 accaagatca atctgtttgc tgaaatggca ggtaaagaag ctgcacagga agaaaaacta
     2821 tccggtttca ttcagaagat cagagagaat acccttgaac tgggcaccaa tatgcgtgac
     2881 tttttatggt caatggatgt gcggatggac aatctgtatg acctcgttga tcgtatgcgt
     2941 gatgccggcg aattgatgct gaccgaaaca ggtatcatgt tcagagctga agggctggat
     3001 ccggagttta agaacgtgcc gctttctcag gaaatgcgca gggtttgcct ccagctgttc
     3061 aaagaggcct ttctcaatac tgccagacat tccggagcta ccaaagcagt tttaaaagta
     3121 actctttccg gacagcagct gcaagtctta tatcaggaca acggaacagg aatagttctg
     3181 aatgaagcag caaagaaagg gcactacgga ttgaaactga tgcaggaaag agcagaaaag
     3241 atcggtggtt cgcttcaaat tgaaagccct gaaaagggag gcacactggt aaccctggat
     3301 gttaaaatac cacagatggg tgatacagaa tgaaattctg tcaccttact tttataccac
     3361 atggacatac aggagagtat tcgggtagcg atcgtggaag acgaacagga gatcagagaa
     3421 ctgctggagc agatcattga cctgtcgccg ggattcagct gcaaaagaac gttttcctcc
     3481 tgcgaagatg ccctgcccgg gttgatagct gatcctcctg atgtggtatt gatggatatc
     3541 aacctgccgg gaatggatgg aatttcaggc acaggaaagc ttaccgaaca gatacctgaa
     3601 ctgaatatca tcatgcttac cattcaggat gaccctgatc tcatttacca gtctctgtgt
     3661 gccggcgcca caggctatct gctgaaagat acacctccgg tcaaactgct gcaggccatt
     3721 gaagaggtca gacagggcgg gtctccaatg tctccttcca ttgcacgcaa ggttaccatg
     3781 tcttttagac cggctgtcaa ctatgacctc tctgacagag aaaaggaaat tctgacccgg
     3841 ctcacacagg gcgaaaacta ccaggtcata gcagcagaac tcttcataag tggtcatacg
     3901 gtcagaaccc atatcagaaa catctaccgc aaactgcagg tcaacacaag aggcgaggct
     3961 gtttccaccg cattgcgcaa taagatcgtt taaaatacct catatgtgcg attgaccggg
     4021 catccttccg gccatagttt tgcattgtaa tttatttcat atctaaaaca caatgcaatg
     4081 aaaacatccg gacttctaac attacttttt gcatttgttg cgctggcggc tgcctgcaat
     4141 aaagagcagg aaccagatcc aaacaacaac aataacaaca ataataacgg gggcaacaac
     4201 ccgcccactg aaacaatgct gagctgcaaa acagatggtg cagactggca atctgatacg
     4261 accaatgtcg ttgcggtcat ggtagaagtg gccggaacca aaacccttac catcagtggt
     4321 gaagcagccg acgggtcttc tctggtattg tccatctcac tatggacagg ggtggaaggg
     4381 agttttccta ccagcctgat gagttttacc aactacgtgg caatggccta tacagatacc
     4441 tccgatgaag cattctctgc tccaaacatc aacaatgccg ccgcaacagg taccctgacc
     4501 atcacctact gggatgaaga aaaagtgagc ggctccttct cttttacagg tgggcaggat
     4561 gatacatcag atacgatgac catctctgaa ggttccttta gtgtaaccca ggtcaattga
     4621 aaaaaatgaa aagagtacac atcatacatc tggttttgtg ggcactcgtt cccaccgccg
     4681 gaatcatggc gcaaccggtt atcggtttgt ccaatatgcc gcaggttggt gattcggtca
     4741 tcattgcgct cagtaaccag cctgttgcac ccggaactgc cggagcagac cagacctggg
     4801 atatgacctg gatatcagca gcagaagaac aatacttcgt ctatgaagat cctgctgtta
     4861 ccccatgggc cgacctgttt gatgacgcta ccatttgtgg tcgctcctgg gaagactact
     4921 acagctacta ccatattgac gccacaggcg tggctgctgt tggatatgcc ggagttatag
     4981 gtctgtctga tacatttacc atggactatt cagacagtga attgtttatc ccgttggatt
     5041 attccttcgg cgattcacat gctgatgact tcgcaggaac cagcaccgct gccggttttt
     5101 ccctgccctt taccggcgat gtggacttcg aggcagacgg ttatggtacg ctgatcctgc
     5161 caacaggtac atatgaaaac gttgttcgat accatttctt cagaacacag gagatcacct
     5221 tcggcactac caccaccaca acaaaggaac aatgggcctg ggtatcttct gactatcgtt
     5281 tctggctgct gcttatggaa gagaactttg acggcttcag caccacccct cttatctggt
     5341 atgataaggc acccctgcct gccgtaccgg tggcaattgg atcagctgaa aattattctg
     5401 tcagattgtt tcctaaccct gtttcagcac aacagtcact gtcttttgaa tggaatgaac
     5461 aaagcaatgc ggtccttaca cttacagatc tccatggccg caaagtctgg cagagaaata
     5521 tggaactgaa acccggcatg caatcacttt cttttgacgg acaggttgac gctggttgct
     5581 acctgttgca aattgcaaca gaaaaagcgg atcatgttac caggctgatc gtgagcgaat
     5641 aaacatacaa tacctgccat gcaggcattt acccggatgt agccggatct catgcaggtc
     5701 cggctgcatc ttgttttata cctcctgtat ggagtatatt agcagatgat tcaaaaatta
     5761 tccagtctct ttgtttggtt gctggccgga ctcatacaga ccgcatctgc acaattcaat
     5821 cacctctggt cctactcgca ttactccaac aataatgacg agataaaagc cattgctgcg
     5881 catgattcag gtactgttgt tactgccggg tcatttatta atacctgcat atttgatgga
     5941 gatggaccct ctctgaacct gacctctgca ggtggaaaag atgtcagcct ggcaagctat
     6001 caggctgatg gtacactcaa ctttgtgatc ggtttcgggg gaagctcaga tgatgttgca
     6061 gctgatgtgg tgttggatgc cgatggtaat atctatgtga ccggttcctt ccagaacagc
     6121 atggatgttg atccgggtgc cggcgaattc attctggaag catcaggcct caacgatgtt
     6181 ttcctgatca aatacaacgc cctcggagaa ctggtctggg cacatcgttt tggagaagat
     6241 tttacagacg atggtaaaga gctggtgctg ggcgacgatg gttttctgta tatgtcgggt
     6301 ggttaccgca ttgaaatgaa atttgcaggc gtagcagatt ccattacact gtccaatccc
     6361 ggatttacca ctgatgtgtt tcttgcaaag ttcaacctgg atggtgaagc cgtctgggca
     6421 agatctttcg gcggtggagg gtctgacttc gtggatggtc ttgcttactg gaatgatatg
     6481 cttttcctca ccggcgattt tacaaccacc atagatctgg atgcttcagc agccgttgat
     6541 aaccatagca gcaatggcct gaatgacgta ttcctctgtg cctatgacac cgatggcgat
     6601 tacctctggg ggcatacctt cggtggatct tctgcagatt ttggtcatga tcttgctacc
     6661 gataatgatg gcaatatcta tatgaccggc aacttcaaca atacgatcaa tttcagttct
     6721 acaggtggtt ctctggagct cacctctgct ctgcaaagcg atatgttcct ggcggcctat
     6781 acagccttcg gcgatgatct ctgggccata cgtgcaggcg gaagtgatgc agaagaggga
     6841 tatgcagtag ctgtatcggc atcaggtccg gtggtaacag gaacattcgg ctcaaccgta
     6901 gattttgatc ccggtcccgg tacatgggag caaacatcag ccgggttcta cgatatctac
     6961 ctcgtacagt acgattttga cggtgcattg atcgaagccg gaaatatggg ctctccctat
     7021 tccgattatg gtcatgtgct gaccatggca ccaaatggag atatcctgac aggaggaaga
     7081 atgcagtcgc cactggacat tgatcccggg ccaggtgaaa tactcttgta cggaaacacc
     7141 ggatttgacg gatacattgc acggtacaat acatgtatct catatttcct ggaagagtca
     7201 gctaccattt gcgccggcga gacctatgat tttcatggta ctgttctgag cgaagcagga
     7261 gaatatgtgg cctcattcac caccgttgat ggctgtgatt ctacctttga actttctctt
     7321 gttattcatg atattgatat aaccattact tcagatgata cagtactcaa tgctgtatat
     7381 tcaccagatt attcctatca gtggctggat tgtaatgatg gtatgacacc tgttgaaggt
     7441 gctacagaag ccgttttctc cgccactgaa tcaggtagct atgctgtaga gatcacatgg
     7501 gggccttgct cagagatctc agcatgtgtt tccgttgata tagaagagcc tgtcggattc
     7561 tcagaagaat tgcacgcaga ctggttgctg tggaatggcc tgaacaactg gatcatcagc
     7621 agcgatcagc cggcacaggt tcaggtattt gatatgcagg gacaagcagt gtacaacagc
     7681 acgatcaacg gaacaacaga gttatctacc ggtcagcttg cagctggatg gtatatcctc
     7741 aaatacagca cagatctcta tcaggacagc aggaagatac tggtgtatta accttactgc
     7801 ttgatataga tcgtaccggc atttcctgcg gtcagcatat agatgccggc aggcagcgaa
     7861 cggatatcca tctgttcatc tgccgaattt ccggtcatga ccaggcgacc gctaaggtct
     7921 tttaccagca ccggaatagg actgttcttg ccgggaaatt ccaggacgaa aagtccgttt
     7981 cccgggttgg gatacaccag taattcttca ggatgtgcat cactttcgtc caggcctgtc
     8041 agcaattcca ggctgtactg gctgaagaag cgccagatct ctgcactggc attgatgtct
     8101 ttattcgtgc tgccgctggc aggtaataac aaaatggtac ctggccaggt atgtcctccg
     8161 ttgatcacct tgaagtgttc tacagagaca ccgttatcac caccagacca tatgtagtgt
     8221 tctactgtac tgccatcgcc cgggtctgta tccggtatct cggtaaactc cggtgtcgtg
     8281 ttgcaattgt tataggatat ccagtagtcc agcagttcat ctatcggcag aaaatcacca
     8341 ctgcctgcat aaggaactgt ggcatcagca gtgccatgta tctccatgac aggagtgggg
     8401 tgggaaggac tgcagtaatc gaagtgatca gtatccatag tacctgtaac cgatgcgatg
     8461 gctgcaatgc ggtcgctcag ctgacaggcc agttcatagc tcatgaatcc tccattgctc
     8521 ataccggtag agtagatcct gttctggtcg atattatatt cggcagagat actgtccagc
     8581 agatcgctga taaaggccac atcatccaca ccggtgccga aagcattgaa ggtgttccag
     8641 tgctgaaatc ccaacacatc ctgggtgcca tctggcagca gaatgaggaa gttcgcagta
     8701 tcggctatcg ggcggaattc accatagaat atctgttgaa acgcatagga accatagccg
     8761 tgcagattca gtaccagggg cctgggttca ctgccatcat aggcagccgg cacatacagg
     8821 taatactggc ggctctgtcc gccggaatgg atgctgtcaa tgacagtggt ctgggcacca
     8881 acagaaacgg aagccaacaa caggatgcag gagagaaatt tcatgggata aatatacaca
     8941 tgaaaaaagc gggctatgcc cgcttttcaa atccaaagat ttggtagtaa gatccaatag
     9001 ctaattagct aattattcag aacctttcgt atgcgctgaa gtgaaagcct gcctcaa
//
LOCUS       DAOMAX010000090         9027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1092_length_9027_cov_4.604882, whole genome shotgun sequence.
ACCESSION   DAOMAX010000090 DAOMAX010000000
VERSION     DAOMAX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_9027_cov_4.604882"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PK742_06275"
     CDS             complement(<1..630)
                     /locus_tag="PK742_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76230.1"
                     /translation="MKRIQLFEFEDFGWLPDSLRSSMTRLLLIFHRMAGTEKVVTDLT
                     AYVLTKVKADHITDLGSGAGGVMPAVVKALNADREMPVRLLLTDLHPNQRSVRLIEAQ
                     KLSWMNYHPEPVDATRMETVPGGLKTMIASFHHLPPGMAKQVLQSASEQKQPLLIYEV
                     AENKIPLIAWWLFLPISLALLIIMSLFMTPFCRPLTWQQLVFTYLIPVIP"
     gene            complement(702..1571)
                     /locus_tag="PK742_06280"
     CDS             complement(702..1571)
                     /locus_tag="PK742_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU76231.1"
                     /translation="MRQLKITKSITNRESESLEKYLQEIGKVDLVTAEEEVLLAQRIK
                     QGDQEALEKLVKANLRFVVSVAKQYQHNGLTLNDLINEGNVGLVKAAQKFDETKGFKF
                     ISYAVWWIRQSIMQALAEQSRLVRLPLNKVGSLSKINKAFSELEQQYEREPTAEELAE
                     CLELGVEEIKNTMSVSTRHVSVDAPFDDGENSSLLDVLENREVEKTDEKLDYTFSLKV
                     ETERTLATLTERERDVIKLFFGIGVPYAMTLEDIGEEFGLTRERVRQIKDNAINKLRS
                     SARNKALKAYLAQ"
     gene            1766..3376
                     /locus_tag="PK742_06285"
     CDS             1766..3376
                     /locus_tag="PK742_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU76232.1"
                     /translation="MLLPVFLGFGLVLPPSDTIGWMAVGEQALSEFGKSVCTAGDVNG
                     DGYDDFLVGQHYFSGPEYREGRVALYFGGPTGPADSPDWTFEANQENATLGISVSTAG
                     DVNNDGYADIILGAHTYSGPLGNEGAAFLFYGSPTGPGSSPDWTFIGDQSGGDFGGSV
                     ADAGDLNNDGYGDVTIGAVFYSNGETGEGRAYVFLGSASGLSLTPDWVQEGQLAGAQF
                     GGDVSGAGDVNSDGYDDLLVGARKYTSTVNSEGGLFLYYGGPSGPSATASWTYFSGAM
                     QAKLGQNVSGAGDVNGDGYADIIAGAHNYSGPETDEGKVYVFYGSPTGPGSTPDFTFE
                     SNLENALSGNQVADAGDVNDDGYGDIIFGSREYSNGETSEGRAFVFYGSSTGLHPEDY
                     WYAESDQEQAFFGYSVASAGDVNGDGADDVIIGAKYYDETLEDEGAAFLYYGTPNPGC
                     TAPADLVAAAITTTSAQLSWNGPAGASGYRIILKRPGHPAILLASTSTTMLLSGLDPG
                     TTYRVVIRAKCGSEFSQRSEPFLLTTVE"
     gene            3456..5858
                     /locus_tag="PK742_06290"
     CDS             3456..5858
                     /locus_tag="PK742_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU76233.1"
                     /translation="MKLHTTHIPFLLSVVLSWTATQADAQSIYTTDWQYSGDIEIEAF
                     GWSLCNAGDVNGDGYDDLLVAAIDHSEPIETEEEEGKLYLFYGGPDGLSATPDWTYQP
                     NDDFTICGFDVSGGDLNGDGYSDIAAGNLQWAGDQIDEGKITLWYGGPDGPGAEPDWI
                     FEGDQDYCLMGSAVALNGDINNDGFADLFVAAKMYDSGEEDEGKVWMFWGSADGPVGP
                     VWSYEPDQDGAICGFPTNYLGDVNADGFDDVIIGVNGYAETWTKQGMAVAFYGSADGL
                     ADTPDWVTYGESKKDYYGHWVDGAGDVNGDGYDDAIVSAILYEDDSTDVNEGAVYFFR
                     GTPSGLQTSYVWKATGNQLEANFGYCISGAGDINADGYADIIVGAKYYTDPEYKEGSS
                     YVFWGGPTGPEDDYCWFAEGGQDSAYLGRHVDGDADFNGDGYSDFLTSAYRYSNILPN
                     DGITYCMYGGPRETDFHLEADTFCINGTDPAPVIDGWPGGTFSASDGLILADTETGVI
                     DLSASSVGVHELTYSYDNGYCVATSTRSVFIGPEVEAGFAYPNDTLFLSGPFPSPNFF
                     PGSSPGVFSAVPEGLIFVSIYTGQINLDLSEPGVYTVTNSVAGDYCNGSYSINIHLIP
                     ECTKPDKPVASDITPTTATITWNAVDWVDDYYVYLIRVGDTTEYDHYPDTILYLTDLL
                     PGTSYRLYVAGYCGGILTQKSENLDFYTQYTEISDVLPAGYQIFPVPAHDLLQVTLPA
                     SGGETRCYITDLQGKPVSGEYVLTGGSTHSLELESIPSGYYQLHLSNAQGYWVSGLSI
                     LH"
     gene            complement(5855..6193)
                     /locus_tag="PK742_06295"
     CDS             complement(5855..6193)
                     /locus_tag="PK742_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HQU76234.1"
                     /translation="MKNVAFVLLLGIFGFSQANAQSTKEKVVIKTSAVCESCKNTIEK
                     AVYQVDGVRSVYLDVETQMATVKFDGTVTHVDAIKKAISQAGYDADELPADKNAYDNL
                     TTCCKKDAVH"
     gene            complement(6217..8412)
                     /locus_tag="PK742_06300"
     CDS             complement(6217..8412)
                     /locus_tag="PK742_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76235.1"
                     /translation="MCFSAAAAQTLNGRVQGMDADQQMTPLPGATLVWLADGSGTITD
                     PDGFFFLDFPGGEDLRLQISFVGYRTDTIKVDTWADVDIMLLSDLILDDVVIAEERSA
                     LAINTSDGVNKITMNERELLKAACCNLSESFETNVTVDAEYTDAVTGTRTIRMLGLDG
                     VYALITADGIPVVRGLASGYGLSYIPGTWIREIDLTKGPGSVENGYEGISGWINARMK
                     SPGPQPAEKLFVNVYGNSLGRWEANANAAAGIGKSLHTGLLVHTMQSQLDVDHNADGF
                     LDMPLGETYTIMNNWVWNNSKHTEIRAGAKWTAQDIQGGQLSFDPQQARDTLNGYGVG
                     LDARRMESYLNAGYSASRPETVFTWHSTGIWHDQSAFYGLSDYSGQETYYRSDLSGGT
                     YIGNTDHLVRAGVSFLYNRFDEQFRDLFMDRTEIVPGWFAQYQFKLKDKVSVMAGYRM
                     DMHNLYGTFQTPRLHLRYSPTTKTTWRASVAKGYRVANVLVENAALLVSSRDLIIADD
                     LLPEEAWNTGITYIRRFPLFNRTSSITADYYHTWFVNQVVTDRDASAQTILIDNLDGR
                     SGSDVAQLEWIVEPVRHLDIRMAYRYTWSYSTFGGELMELPLVNRHRAMLNASWNWQE
                     KGWQADATLHYFGSARLPDLSDNLLAADVSGRSPAYVQLMGQLTRSWDTFSWYAGVEN
                     ATGYTQQDPIIGAGDPFGPNFDASVLYAPLMAARIYSGIRLVIQEKQEQ"
     gene            complement(8500..8910)
                     /locus_tag="PK742_06305"
     CDS             complement(8500..8910)
                     /locus_tag="PK742_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76236.1"
                     /translation="MKRLSQRIASVLLLLLVIGTTTGVQLYSHICMTSGTAEVSMQQI
                     HSCCSETGASSASATIENDCCSSGVSYLKLDLPAWQPDHDFQVLVQHIVLDLPAANTI
                     TGQDAVLCSRYFDPPPATALDGRDHQRLYQVFLI"
BASE COUNT         2204 a         2274 c         2218 g         2331 t
ORIGIN      
        1 aggtatgacc ggtatcagat aagtaaatac cagctgctgc catgtcaaag gcctgcagaa
       61 cggggtcatg aataaagaca tgataataag cagtgccaga cttattggaa gaaataacca
      121 ccaggctatc aagggtatct tgttttcggc cacctcatag atcagcaacg gttgtttttg
      181 ctcacttgca ctttgaagaa cctgctttgc cattccaggt ggaaggtgat ggaaacttgc
      241 gatcatggtt ttcagacctc ctggcactgt ttccattctg gtagcatcta cgggttcagg
      301 atgatagttc atccaggaaa gcttttgtgc ttcgatcaat ctcactgacc tctggttcgg
      361 atgcaggtct gtaagcagca gtctgaccgg catctccctg tcagcattca gggccttcac
      421 cactgcgggc atgacaccac cggcacctga acccagatcg gttatatggt cagctttgac
      481 ctttgttaat acatatgctg tcaaatcagt cactactttt tcagtacctg ccatgcggtg
      541 aaagatcagc agtaacctgg tcatcgacga acgcaaagaa tcaggcaacc agccaaagtc
      601 ttcaaattcg aacagctgga tacgtttcat gcaaattgtt ttttgtaaaa ataaaacagc
      661 ccgccaaagt gggcgggctg aattttaatt gttgctgcag atcactgtgc cagataggct
      721 ttcagcgcct tgttccttgc agaggaacgc agcttgttga tggcgttatc cttgatctga
      781 cgaacccgtt cacgggtcag accgaactct tcgcctatgt cttccagcgt catggcataa
      841 ggaactccta taccaaagaa gagtttgatc acgtcgcgtt ctctttcggt cagggtggcc
      901 agagtacgct cggtctccac tttcagtgaa aaggtataat ccagtttctc atccgtcttc
      961 tctacttcgc ggttctccag cacgtcgagc agggaagagt tctcaccatc atcgaatggc
     1021 gcatccacac tgacatgacg ggtgctcaca ctcatggtgt tcttgatctc ttctaccccc
     1081 agttccagac attcagccag ttcttctgcc gtaggctcac gctcatactg ctgttccagt
     1141 tcactgaatg ccttgttgat cttgctgagg cttcctacct tgttcagagg cagacgaacc
     1201 aggcggcttt gctctgccag tgcctgcatg atgctctgac ggatccacca tacggcgtaa
     1261 gaaatgaatt tgaaaccttt ggtctcatcg aatttttgag cggccttcac caggcccaca
     1321 ttgccttcat tgatcaggtc gttcagggtc agtccgttgt gctggtactg cttggccacc
     1381 gataccacga aacggaggtt ggcctttacc agcttttcca gtgcttcctg gtccccctgc
     1441 ttgatccgtt gggccaggag aacctcctct tcggcggtca ccaggtccac tttcccgatc
     1501 tcctgcagat atttctccag cgattcactt tcacggttgg taatggattt ggtaatcttt
     1561 aactgacgca tagtgtagtt tattttgtta tggcacgagg caagtaagag ttatacgaag
     1621 cagaatcata aaagtttcag taaaaaccct gttttttagc cctttttttg ccgaattaca
     1681 gccgggggag tgcaaaagta acatctttgg tcagctgaat caacccctgc tgtttatcat
     1741 acgtcttacc ttataacttt gttccatgtt gttacctgtt tttctgggct tcgggctggt
     1801 attacctccg tctgacacca ttggctggat ggctgtgggt gaacaagccc tgagcgaatt
     1861 cggcaaaagt gtttgcaccg ccggcgatgt gaacggcgat ggctatgacg atttcctggt
     1921 tggtcagcac tatttttccg gccctgaata ccgtgaagga agggtagccc tttattttgg
     1981 cgggcccaca ggacctgcag acagtcctga ctggaccttt gaagccaacc aggaaaacgc
     2041 cacccttggc atatctgtgt caaccgccgg tgatgtcaac aacgacgggt atgctgatat
     2101 catcctcggt gcccatacct acagcggccc cctgggtaat gaaggagccg cctttctctt
     2161 ctatggtagc ccgacaggtc cgggtagcag ccccgactgg accttcatag gcgaccagtc
     2221 tggcggagat tttggcggat ctgtagcaga tgccggcgac ctgaataacg atgggtatgg
     2281 cgatgtgacc attggagctg tattttattc caatggcgaa acaggcgaag gacgtgccta
     2341 tgtgtttctt ggcagtgcct ccggcctctc actgacccct gactgggtgc aggaaggtca
     2401 acttgccggt gcacagttcg gtggcgatgt gtcaggcgca ggtgatgtga acagcgatgg
     2461 gtatgatgac ctgctggtgg gtgccagaaa atataccagt acagtaaaca gcgaaggagg
     2521 tctgttcctg tattacggcg gcccttccgg accctcagcc acagcttcct ggacctactt
     2581 ttcaggtgcc atgcaggcca aacttggtca gaatgtttca ggtgccggag atgtcaatgg
     2641 cgatgggtat gctgatatca tagccggtgc gcataactac agcggacccg aaacagacga
     2701 aggaaaggtc tatgtctttt acggctcgcc cacaggaccg ggaagtaccc ctgattttac
     2761 ttttgaatcc aacctggaaa atgccctcag tggaaaccag gtggctgatg caggtgatgt
     2821 gaacgacgat ggctatggag atatcatctt cggcagccgt gaatacagca acggtgagac
     2881 cagcgaagga agagcttttg tgttctacgg cagcagcact ggcctgcacc ctgaagatta
     2941 ctggtatgca gaaagtgatc aggaacaggc attcttcggg tacagcgtgg cttctgccgg
     3001 cgatgtgaac ggcgatggag cagatgatgt gatcatcggt gccaaatact acgacgaaac
     3061 cctggaagac gaaggtgcag cctttctgta ctatggaact ccaaacccgg gctgtaccgc
     3121 tcctgcagac ctggtcgctg ctgctatcac cactacttca gcccagctca gctggaacgg
     3181 acctgcgggt gcctctggct acaggatcat tctgaaaaga cccggacacc ctgctatcct
     3241 gctggccagc accagcacca ccatgttgtt gagcggcctg gatccgggta ccacctacag
     3301 ggtggtgatc agggcaaaat gtggcagcga attctcacaa agatctgaac cgtttttatt
     3361 gacaaccgtt gaataattgg ataattttag aggacgttag gatccggtca tacatgttta
     3421 attgtcttta tatgttataa aatattaatt cgtctttgaa actacatact acacacatac
     3481 catttctgct gtctgttgtt ctgtcatgga ctgccacaca ggcagatgct caatccattt
     3541 ataccactga ctggcaatac tcgggcgata ttgaaataga agcattcggc tggtcattgt
     3601 gcaacgccgg cgatgtgaac ggcgatgggt atgatgacct cctggttgcc gccatagacc
     3661 attcagaacc cattgaaaca gaagaagaag aaggtaaact ttatctcttc tacggaggtc
     3721 ctgatggtct gtcagctaca cctgactgga cctatcagcc caacgatgat tttaccattt
     3781 gcggtttcga tgtaagtgga ggcgatctga acggcgatgg ctacagcgat attgccgcag
     3841 gaaatcttca gtgggccggc gatcagatcg atgaaggaaa gatcacactt tggtatggcg
     3901 gaccggatgg cccgggggcc gaacctgact ggatctttga aggcgaccag gattactgcc
     3961 tcatgggaag cgcagtggcc ctgaacggag acatcaacaa cgatggtttt gccgacctct
     4021 tcgtggcagc caaaatgtat gacagcggcg aagaagatga aggcaaagta tggatgttct
     4081 ggggcagtgc cgatggtcct gtcgggccgg tatggagtta tgaacccgat caggatggtg
     4141 cgatctgcgg tttccctacc aattatctgg gcgatgtcaa tgcagatggt tttgatgatg
     4201 tcattatcgg tgtgaacggt tatgctgaaa cctggaccaa acaaggaatg gctgtggcat
     4261 tctacggaag tgccgatggc ctggcagata ctcctgactg ggttacctac ggagaaagca
     4321 aaaaagatta ttacggccac tgggttgatg gcgcaggcga tgtcaatggc gatggttatg
     4381 acgatgccat tgtctctgct atcctgtatg aagatgattc aacagatgtc aatgaaggtg
     4441 cagtgtattt tttccgtggt acaccttccg gtttacagac cagttatgtc tggaaagcta
     4501 ccggcaatca gctggaagcc aactttggat attgtatctc cggtgcaggc gatatcaatg
     4561 cagatggtta tgcagatatc attgtgggtg ccaaatacta tactgatcct gaatacaagg
     4621 aaggctcttc atacgttttc tggggcggac caaccgggcc agaagatgat tattgttggt
     4681 ttgccgaagg cggacaagac tcagcatacc ttggcagaca tgttgacggc gatgctgatt
     4741 ttaatggcga tggttactct gacttcctga caagcgccta ccggtattcc aatatcctgc
     4801 cgaatgatgg catcacttat tgcatgtatg gcggcccacg ggaaacagat ttccacctgg
     4861 aagctgacac cttttgtatc aatggcactg accccgctcc tgttattgat ggctggccgg
     4921 gtggtacttt ttcagcctcc gatggtctga tcctggctga tacagaaacc ggcgtcatag
     4981 atctttcagc atcttcagtg ggagtgcatg aactgaccta tagttatgac aacggttact
     5041 gtgtggctac cagtacccga tcagtgttca tcgggcctga agtagaagca ggttttgcct
     5101 acccgaatga taccctgttc ctgagcgggc cgtttccttc accgaatttt tttcccggtt
     5161 catcacccgg agttttcagc gctgtacctg aaggcctcat tttcgtgagc atctataccg
     5221 gccagatcaa tcttgacctg tctgaacccg gggtctatac ggtaaccaac agtgtagccg
     5281 gcgactattg caacggcagc tattccatca acattcacct gatacctgaa tgtaccaagc
     5341 ccgataaacc ggttgcgtct gatattactc cgaccaccgc aaccataacc tggaatgctg
     5401 ttgactgggt agatgactac tatgtgtacc tgatccgggt gggagatacc accgaatacg
     5461 atcattaccc agataccatc ctgtatctga cagacctctt gcccggtact tcttatcgtc
     5521 tgtatgtggc cggctactgt ggaggcatcc tgacacagaa atcagaaaat ctggatttct
     5581 atacccaata tacagagata tcagatgtac tgccggcagg ctatcagatc tttcccgttc
     5641 ctgcacatga tttgttacag gttacgctgc cggcttccgg tggcgaaacc aggtgttata
     5701 tcactgatct gcagggcaaa ccagtgtctg gtgaatatgt attaacggga ggttccaccc
     5761 acagcctgga actggagtcc atcccgtcag gatactacca gttacacctc agcaatgcac
     5821 agggttactg ggtttccgga ctgagcatcc tgcactaatg cactgcatcc tttttacagc
     5881 aggtggtcag attatcatag gcatttttat ctgccggtaa ctcatctgca tcataacctg
     5941 cctgactgat cgctttcttg atggcatcca catgggtgac tgttccatca aacttgacag
     6001 tagccatttg ggtttcaaca tccagataca cagacctgac accatccacc tgataaaccg
     6061 ccttttcaat ggtgttctta caagactcac ataccgctga ggtcttgatg actaccttct
     6121 ctttggtgct ttgtgcattt gcctgtgaaa agccaaagat gccaagcaat aaaacaaacg
     6181 ctacattttt catatttcaa tttttgaatt tattaattat tgttcttgtt tttcctgtat
     6241 gaccaatcgg atacctgaat agattcttgc agccatcaat ggtgcataca ataccgaagc
     6301 atcaaaattc ggtccgaacg ggtcaccggc accaatgatc gggtcctgct gggtataacc
     6361 ggtggcattc tccacccctg cataccagct gaatgtatcc catgacctgg taagctgtcc
     6421 catgagctga acataagcag gcgaacgtcc ggaaacatct gctgcaagca gattatccga
     6481 cagatcaggc agtctggcag aaccgaagta atgcagggtg gcatctgcct gccagccttt
     6541 ctcctgccag ttccacgaag cattcagcat ggccctgtgt ctgtttacca gaggcagttc
     6601 catcagctca cctccgaatg tgctgtatga ccaggtatat ctgtatgcca tgcgaatatc
     6661 caggtgtctt acaggttcca caatccattc cagttgtgct acatcgctgc ctgacctgcc
     6721 atccagatta tctatcagaa tggtttgtgc acttgcatcg cgatcagtaa ccacctgatt
     6781 aacgaaccag gtatggtagt aatctgccgt gatggaagag gtgcggttga aaagcggaaa
     6841 tctcctgata tacgttattc cggtattcca tgcttcctca ggcagcaggt catcagctat
     6901 gatgagatca cggctgctca ccagcagggc agcattctcc accagcacat tggcaacgcg
     6961 gtaacctttg gctacagaag cccgccaggt ggtcttggtg gtagggctgt atctcaggtg
     7021 caacctgggt gtctgaaagg ttccgtagag gttatgcata tccatacgat aacccgccat
     7081 cacactgacc ttatccttca gtttgaactg gtattgtgca aaccaaccgg gcacgatctc
     7141 ggtcctgtcc atgaacagat cgcggaactg ctcatcaaac cggttgtata aaaaggaaac
     7201 acctgccctt accaggtgat cggtattacc gatataggtg ccgcccgaaa ggtcagatct
     7261 gtaataggtt tcctgtccgg aatagtcact cagaccataa aaggcagact gatcgtgcca
     7321 gatcccggta ctgtgccagg tgaagaccgt ttccggcctc gaagcactgt aacctgcatt
     7381 cagataactc tccatgcggc gggcatccag acccacgcca taaccattca gggtgtctct
     7441 tgcctgctgc ggatcaaatg aaagctgccc tccctgtata tcctgtgcgg tccactttgc
     7501 accagctctg atctctgtgt gtttactgtt gttccagacc cagttgttca tgatggtata
     7561 ggtctcaccc agcggcatgt ccagaaaacc atcggcatta tgatcgacat ccagttggct
     7621 ttgcatagta tgcaccaata agcctgtatg cagagactta ccaatgccgg ctgcagcatt
     7681 ggcattcgct tcccatcgcc ccaatgaatt accatagaca ttgacaaaga gtttttcagc
     7741 aggctgaggt ccgggcgatt tcattctggc attgatccat ccgctgatgc cttcatagcc
     7801 attctctaca gagcccggtc ccttggtgag atcgatctct ctgatccagg taccaggaat
     7861 ataggacaga ccatagccgg atgccagccc ccggactacc ggtatgccat cggctgtgat
     7921 cagggcatag accccgtcaa gacccagcat gcggatggtt cgtgtgccgg taactgcatc
     7981 tgtatattca gcatccaccg tcacattggt ttcaaaactt tcgctcagat tgcagcaggc
     8041 agctttcagc aattcacgtt cattcatcgt gatcttgttg accccatcgc tggtattgat
     8101 ggccagtgca gatctctctt ctgctatcac tacatcgtcc agtatcaggt cactcagcaa
     8161 catgatgtcc acgtcagccc aggtatccac ttttatggta tctgtccggt agccaacaaa
     8221 actgatctgc aacctcaggt cttcaccccc aggaaaatcc aggaagaaaa agccgtcggg
     8281 atcagtgatc gtgccggagc catcagccag ccagaccaaa gtggcacccg gaagaggagt
     8341 catttgctga tctgcatcca taccttgcac cctgccattc agggtctgcg cagcagctgc
     8401 agaaaaacac aaagtactca agataattat tatatatttc atgatctaca tattaacgtg
     8461 aataatatcc tccgttcagg aggatgacct cacttcatat cagatcagaa atacctggta
     8521 gagacgctgg tggtctctgc catccagagc ggtggcaggc ggtgggtcaa aataccggga
     8581 acagagaaca gcatcctgtc ctgtaatggt attggcggca ggcagatcga gtacgatgtg
     8641 ttgcaccagt acctggaaat catgatcagg ctgccaggca ggcagatcca gtttcagata
     8701 ggatactccg gatgagcagc aatcattttc tatggtggca gaagctgaag aagcccctgt
     8761 ttcactgcaa caagaatgga tctgctgcat agagacctca gcggtgccac ttgtcatgca
     8821 gatatgcgaa tacaactgca cacccgtggt ggttccgatc acgagcaaca ggagtaaaac
     8881 agacgctatg cgttgtgaca gacgcttcat gaacttcaaa gttaccagtt ttacaaaaaa
     8941 ctgacccgga tatttgtcat ttggtgattt actgcacgtt attccggaat caggtgttgt
     9001 acctttaaga acgataaaaa acgaaag
//
LOCUS       DAOMAX010000091         9015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1093_length_9015_cov_4.051674, whole genome shotgun sequence.
ACCESSION   DAOMAX010000091 DAOMAX010000000
VERSION     DAOMAX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9015
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_9015_cov_4.051674"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK742_06310"
     CDS             <1..597
                     /locus_tag="PK742_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76237.1"
                     /translation="SLVGSYATILATLYAGIIALMVFLRQQDLLGGPSFTIIVFLSVI
                     LLALLTLAYYYLYLVVDLLRNRRIMRRLVRYIEVLSFYSPADFTRIFLLSLLRYSVFS
                     AQYLILLRLFGVHLQIGEGWTAVSVIFLAQTALPSFTVAELFTRGNISLYFLRYYTDN
                     TGAILAASTSLWLLNLIFPAVAGYFFILRKNFFKTRNQ"
     gene            594..1433
                     /locus_tag="PK742_06315"
     CDS             594..1433
                     /locus_tag="PK742_06315"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQU76238.1"
                     /translation="MRKATLFIPVLLATAMMTSAPDTKVVPGGEMCQVQKDAFQAGES
                     LTYKVYYNAGPMWVGAGEVYFKLHDIDHKGKKTLYGVSEAVSYSSFDWFFKVRDKLET
                     YMDPVTLQSYEFKRDVYEGGYTFYRSYDWDRKTNTIISFEDRKNGKTKTETITGVDPC
                     ATDLLSVFYWARTIDFEKYKVGDKIPVSLAADDKVYDIYIKYEGKEEYKTKQGTFRCM
                     KVKPLLVEGQVFRSGEGMTIWFTDDDNRIPVRIESQLLVGRITCDLKSYSGIKYPFTA
                     KVK"
     gene            1430..1720
                     /locus_tag="PK742_06320"
     CDS             1430..1720
                     /locus_tag="PK742_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQU76239.1"
                     /translation="MIKQLSLQELEEWRLEGRPFRLIDVREDDEREVFHIGGEHMPFG
                     SVQNNFKPINSNEIVVFYCRHGVRSRIVISRLQSLYPDAQFYNLSGGIGDQE"
     gene            complement(1711..2616)
                     /locus_tag="PK742_06325"
     CDS             complement(1711..2616)
                     /locus_tag="PK742_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQU76240.1"
                     /translation="MAHSPRWRPLSSGSAIGIISTARAIMQDDIREAISMIQARGFQV
                     KLGRSIGVSDDQFAGSDELRANDLKNFLEDQDIEAILLARGGYGTMRTLNQLSEDVWQ
                     SHHKLIIGYSDVTVLHGWLNTRLGTPSLHATMPVSFTTHSAASVETLLDSLLGKLPEY
                     RIPAHLKNRDGLAKGVITGGNLSILYSMLGTRWDWDASGMILLLEDVDEYLYHIDRMM
                     VALYSAGKLERLAGVIVGGMTDMHDNAIPFGMDATSIIASWLPENIPVCMGFPAGHQS
                     DNLAVPFGIEATLEVNSSGGHLYYS"
     gene            2644..4701
                     /gene="metG"
                     /locus_tag="PK742_06330"
     CDS             2644..4701
                     /gene="metG"
                     /locus_tag="PK742_06330"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQU76241.1"
                     /translation="MNTPARYLITAALPYANGPLHIGHIAGAYLPADIYVRYLRMQKH
                     DAVFVCGSDEHGVAITIQAKKEGVSPKEIIDKYHEINAASFRELGIDFDIYHRTSSAL
                     HHETSSAFFRTLYDKGVFEERESEQFYDEEYKQFLADRYIIGTCPKCQNPNAYGDQCE
                     RCGTSLSPDDLIEPRSAMSGKQPVKRQTRHWYLPLDKYQGWLRSWLVDGEGRQEDWKK
                     NVLGQCKSWIEEGLHPRAITRDLDWGVPVPVEGAEGKVLYVWLDAPIGYISATRQWAM
                     DHQKDWTPYWQDASTKLVHFIGKDNIVFHCIIFPVLLKAHGGYILPTNVPANEFMNLE
                     GEKMSTSRNWAVQVHQYLQDFPDQQDVLRYVLATNLPEAKDSEFTWKDFQARNNSELV
                     AILGNFVNRVLVLTHKFAEGRIPEFNEVFSSQEYIVSYRENMRFLLEETYKPAMEQYR
                     FREALAAAMDMVRSGNKLLTDFEPWKIFKSDPTTCLAVLRVCLDSLADISLVMHPFLP
                     FTAGKIRSMIGLNDLFDLDSFASPELETGRDIAPAIHLFTPVEDATVQQQVDNLGRNK
                     TVTEPEKPVSKPMIQFEDFSKLELKTGVITAAETIPKADKLLKLQVDLGSETRTIVSG
                     IAAHFKPDSLAGQRVVVVTNLEPRKMRGVESQGMILMAEDPSGQLFFVGTGPDGLGGW
                     NVS"
     gene            5095..7422
                     /locus_tag="PK742_06335"
     CDS             5095..7422
                     /locus_tag="PK742_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQU76242.1"
                     /translation="MGFSDDEINSIMGSVGAPAEPASTNAMEQTNQLLNEEGQQQQGN
                     SQQGEQQQQEEQQQQEQQQESEQQQAQSQDQGSSGPPPATIYGQNFFRTGNAELFDRV
                     PNAKVNDNYIMGSGDQIVISVYGGVSSYNESFTVSDDGNIDVYGIGKIYVKGLTYAGA
                     KKLLRQKYSGYINLAGSSFDVNLIYSKAIKVNIVGEVITPGSYNISSINTAYNALTAA
                     GGPNDIGSVRNIQIKRAGEVVKTLDVYEFLMNPGNTDDTYLMNNDYIVVTPVGKVVEI
                     AGEVKRPFRYELKEGENLMELLEYSGGLKGTAYTRTVTIKRYAENENQVIDINLDSLR
                     LQGKDYELIDGDMITIAKIPEVVENVVSITGAVRFPGTYQLSEGLRISDLVDKAKGLN
                     YDAYLTRAYLIRKNEKMNEVYIPFDLKEVMDDPNSVFNFELTKFDVIEVFSKDRFREV
                     FNVSIEGAVKAPGQFSYYDKMTLKDLLYYAGGLRVEAANNKIEISRIVNFNEAKDQNE
                     PTRVVIESISIGKELELTDEAENFIIQPYDHVYIRTTPEFELQKMVTVQGEVIYPGNY
                     TLLSKTETVADVIERAGGVTQYAYVEGATIKRNNVTNTLLFLDKAMKDPESKYNYVLR
                     DLDVIVIPRQGDLVSLEGAIEFPFEISEADQETGQVMVPYDKGRTARFFIKKYGKNFD
                     DDAERGKTYVVQPNGYVKRTHNFLWFIHFYPHVKVKGSTVVVPYKEKEKEEPEQTDTV
                     AAEPFDWNVFMATISASILSFATIYVLINNSNRNN"
     gene            7445..8590
                     /locus_tag="PK742_06340"
     CDS             7445..8590
                     /locus_tag="PK742_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76243.1"
                     /translation="MQYERPYNDEINIGDLITKLGEYRKYLVKKWWVILVSALVVGMA
                     LRFYINWRPEHYISHQDFSVKGTEGESTSTLGSLASSFGFGISTGAEFTNEYFLAIMQ
                     SRRLIKETLLQERTIQLDKQEPRTDYLVNFYLDMYPRWAKKKKLKKFRMDHGNIDSLT
                     IYEDSCLSIIYDEIIDHDLTVDYTDDLDLNQLEFSSINRQFSLDFANYLANAASDFYI
                     NSQIVVEVETVSMIQHRADSIRAVMESKEDQLAALQDRTAFTIKAAGLINQGRLLRDI
                     ELLNLEYGEVFAQLELAKFDLRNKTPLVSVVDSPRYSTIKEKEQIMLFMIIGFVLGIV
                     ISSIVLVIRKYTRDSVAEAKQKQELIRQYEEQQAAVLLEQKENREIE"
     gene            8731..>9015
                     /locus_tag="PK742_06345"
     CDS             8731..>9015
                     /locus_tag="PK742_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76244.1"
                     /translation="MSWKPEYAFLIVASTAIDYYSGMRMSAITDKKKRRPFLMLSIFT
                     NLSLLLLFKYFNFFNESARAVFDSFNIFYNVPEFNMFLPVGISFYTFQTLS"
BASE COUNT         2476 a         1949 c         2171 g         2419 t
ORIGIN      
        1 tcactggtag gaagctatgc cactatcctg gctaccttat atgcaggtat tattgcctta
       61 atggtatttc ttcgccaaca ggatttgctt ggagggcctt cctttaccat cattgttttt
      121 ctttcagtga tcctcctggc gctgcttaca cttgcttact actatctgta cctggtagtt
      181 gacctgttgc gaaacaggcg tatcatgaga cgtctggtca ggtatatcga ggtgctttct
      241 ttctactcgc cagcagactt tacccgtata ttcctgcttt cacttctgcg ctattctgtg
      301 ttctcggcac agtatttgat tttactacgg ttgtttggag ttcatttaca gattggagaa
      361 ggttggacgg cggtcagtgt aatatttttg gctcagacag ctttgcctag ttttactgtt
      421 gcagagttgt ttaccagagg taatatttcg ttgtacttcc tgcgttacta cacggacaat
      481 acaggagcta ttctggccgc atcgacttcg ctgtggttgc tgaatctgat atttcctgcg
      541 gtagccggtt actttttcat tctgcgtaag aacttcttta aaaccagaaa ccaatgagaa
      601 aagcaacact gttcattccg gttctactag cgacagctat gatgacctct gcacccgaca
      661 ccaaagtggt acccggtggt gagatgtgtc aggtacagaa agatgctttc caggcaggcg
      721 agtcattgac ctataaggtc tattataatg ccggcccgat gtgggtcggg gctggtgagg
      781 tctattttaa acttcatgac attgaccata aaggaaagaa aacactgtac ggtgtttcag
      841 aagctgtttc ctattcttcc tttgactggt tcttcaaggt gcgagataag ctggagacct
      901 atatggatcc ggttactctg cagagctatg agttcaagcg tgatgtgtat gaaggtggtt
      961 atacgttcta tagatcttat gactgggaca gaaagaccaa taccatcatc tcttttgaag
     1021 acaggaaaaa tggaaagacc aagacggaaa ccattaccgg tgtggatccg tgtgccactg
     1081 acctgctgtc tgttttctac tgggcccgaa ccattgattt tgagaagtat aaggtaggag
     1141 ataagatccc tgtctccctg gcagctgatg acaaggtata tgacatctat atcaagtatg
     1201 aaggcaaaga agagtacaag accaaacaag gtacatttcg ctgtatgaag gttaaacctc
     1261 ttttggtgga aggtcaggtg ttcagatccg gagaaggtat gaccatctgg tttaccgatg
     1321 atgacaaccg cattccggtc aggatagaat cgcagttgct ggttggacgt atcacctgtg
     1381 accttaaatc gtattcaggc attaaatatc ctttcaccgc caaagtgaaa tgatcaaaca
     1441 actttcttta caggaactgg aagagtggcg gctggaaggc cgccctttcc gtttgataga
     1501 tgtgcgggaa gatgatgaaa gagaagtgtt ccatatcggt ggtgaacaca tgccttttgg
     1561 ttctgtgcaa aacaatttca aaccgatcaa cagtaacgaa attgtagtct tctattgcag
     1621 acatggggtc agaagcagaa tagttatatc cagattacag tcactctatc ctgatgcgca
     1681 gttttataat ttgtctggcg gaattggtga tcaggaataa tacagatgtc cgcccgagct
     1741 gtttacctcc agtgtggctt ctattccgaa cgggaccgca aggttgtctg actgatgacc
     1801 tgccggaaat cccatgcata ccggaatgtt ttccggaagc caggatgcga taatggatgt
     1861 cgcatccatc ccaaaaggta tggcattgtc atgcatatcg gtcatccccc ccactatcac
     1921 tccggccagc ctttccagtt ttcccgcact gtaaagagct accatcatcc tgtcaatatg
     1981 atacaggtat tcatccacat cttccaacaa caggatcatt cccgaagcat cccagtccca
     2041 tcgtgtgccg agcatactgt acaggatgga caggtttcct ccggttatta ccccttttgc
     2101 caaaccatcc ctgttcttca aatgagcagg tattcggtat tcaggaagct tccccagcaa
     2161 actatccagc agtgtttcta ccgatgctgc agaatgggtt gtgaaactga ccggcatggt
     2221 ggcatgcaga ctgggagtgc ccaggcgggt attcagccaa ccatgcaata cagtcacatc
     2281 agagtagcct atgatcagct tatggtgtga ttgccagaca tcttcagaga gttgattcaa
     2341 tgttcgcatg gttccataac cacctcgggc caggagtatg gcctctatgt cctggtcttc
     2401 cagaaagttt ttcaggtcat tggcacggag ttcgtctgat ccggcaaact gatcatcaga
     2461 tacacctata ctcctgccaa gttttacctg aaagcctctg gcctgtatca tagaaatggc
     2521 ctctctgata tcatcttgca tgatagcacg ggcagtgctt ataatgccta ttgctgaacc
     2581 ggatgacagg ggtctccaac ggggtgaatg tgccaagctt tcgtaatttt aaggcccaaa
     2641 ataatgaata cacccgcaag atatctgatc acagctgcat taccttatgc aaatggtcct
     2701 cttcatattg gtcatattgc cggggcatat cttccggccg acatttatgt gcgttacctg
     2761 cgaatgcaaa aacatgatgc tgtttttgtt tgcggcagcg atgagcatgg agtagcgatt
     2821 actattcaag ccaaaaagga gggggttagt cctaaagaga tcattgacaa gtatcatgag
     2881 atcaacgcag cctctttcag ggagttgggt attgactttg atatttacca cagaacatcg
     2941 tcggcattac accacgaaac ttcatcggca ttctttcgga ctttatacga caagggtgta
     3001 tttgaagagc gggaaagtga acagttttat gatgaagagt ataagcagtt cctggcagac
     3061 aggtacataa taggcacctg cccgaaatgt cagaacccga atgcatatgg agatcagtgt
     3121 gaacgatgtg gtacttctct cagtccggat gacctgatag aaccacgttc tgccatgagt
     3181 ggaaagcagc ctgtcaagag acagaccagg cattggtacc tgccacttga taagtatcag
     3241 gggtggcttc gcagctggct ggtggatggt gaaggaagac aggaagactg gaaaaagaat
     3301 gtgcttggtc aatgcaaatc gtggatagaa gaaggtttgc atccgcgggc gatcacccgt
     3361 gatctggact ggggtgtgcc tgttcctgta gaaggtgccg aaggtaaggt cctgtatgta
     3421 tggcttgatg cccccatcgg ctatatcagt gctacccgac agtgggcgat ggatcatcag
     3481 aaggactgga ccccatactg gcaggatgct tctaccaagc tggtccactt tatcggtaaa
     3541 gacaatatcg tttttcattg tatcatattc ccggttcttc tgaaagcaca tggcggatac
     3601 attcttccca ctaatgtgcc tgccaatgag ttcatgaatc tggaaggaga gaaaatgtca
     3661 accagcagga actgggctgt tcaagtgcat caatatctgc aggattttcc tgatcagcag
     3721 gatgtgttga gatatgtgct ggccaccaat ctgcctgagg caaaagacag tgagttcacc
     3781 tggaaggatt ttcaggccag gaacaatagc gaactggtag cgatacttgg gaattttgta
     3841 aatcgggtac tcgtgctgac ccataaattt gccgaaggaa ggataccgga gtttaatgag
     3901 gtattttcaa gtcaggagta catcgtttcc tatagggaaa acatgcggtt tctgctggaa
     3961 gagacctaca agccggcaat ggagcaatac cggttcaggg aggcacttgc tgccgctatg
     4021 gatatggtgc gctcaggtaa taaactcctt actgatttcg agccctggaa aatattcaaa
     4081 tctgacccta caacctgcct ggctgtattg agggtttgtc tggattcact tgcagatatt
     4141 tcgctggtaa tgcatccttt cctgcctttt actgccggca aaattcgaag tatgatcggt
     4201 ttgaatgacc tctttgacct tgactcattt gccagcccgg aactggaaac aggtcgtgac
     4261 atagccccgg ctatacacct gttcactcct gttgaagatg ctacggttca gcagcaggtg
     4321 gataatctag gccggaataa aacagttact gagcctgaaa aaccggtatc caagcctatg
     4381 atacaatttg aagatttttc taaactggag ctcaaaaccg gtgtgatcac tgcggcagag
     4441 actattccta aggctgataa gttgcttaaa ctacaggttg atctggggtc agagacccgc
     4501 accatcgtat ctggcattgc ggctcatttt aagccagaca gccttgcagg ccaacgggtg
     4561 gtggttgtta ccaatctgga acccaggaaa atgcggggcg tggaaagcca gggcatgatc
     4621 ctgatggcag aagacccttc cgggcaactt ttctttgtcg gaaccggccc tgatggcctg
     4681 ggaggctgga atgtaagctg atagctgatc cgtcccaact tcagatattc cgggcttgta
     4741 ttgcaacgga agctacatca acagcgtctt acaggggtct tataaggcca attttccact
     4801 tttcaggcag aaaaaagtat tctgttttac aggaatggaa gtttgctttt actcgtatct
     4861 ttgcacgaaa ttttaaaacc acctatgaag gatctgctac ggatatcact gtatatcccc
     4921 tttatttttg cttcggtatc cttatcagcc cagacaggca cacagagttc ctctcagagt
     4981 actcaaggct cctcccagag cactcaaggc tccagccaga gctcgcaggg ctctcctgca
     5041 ggagctgtaa gtgtacctgg tgttggttct atctctgaat ctaccctaca gtctatgggt
     5101 ttcagtgatg atgagatcaa ttccatcatg ggttctgtag gtgctccggc tgaacctgca
     5161 tccaccaatg ccatggagca aaccaatcag ctgctgaatg aagaagggca gcaacagcaa
     5221 ggaaacagcc agcagggtga acaacagcag caggaagagc aacaacagca agagcagcag
     5281 caggaaagtg agcaacaaca ggctcagtcg caggatcagg gttcaagcgg accacctcct
     5341 gctaccatct acggacagaa tttcttccgt acaggaaatg ctgagttgtt tgacagggtg
     5401 cccaatgcta aagtgaatga taattatatc atgggcagcg gagatcagat cgtgatctcg
     5461 gtatatggag gtgtatccag ctataatgaa agttttacgg taagtgatga tggcaatatt
     5521 gatgtgtatg gcattggcaa gatatacgta aagggactta cctacgcagg ggctaagaaa
     5581 ctgttaaggc agaagtacag cggttacatc aatctggcag gaagtagttt tgatgtcaat
     5641 ctgatctact ccaaagccat caaggtgaat attgttggag aagtgatcac acccggttcc
     5701 tataacatct cttctatcaa caccgcatat aatgctctga ccgcagcggg tggccctaat
     5761 gatataggct cagttagaaa tatccagatc aaaagagctg gtgaggtggt caagaccctg
     5821 gatgtgtatg aatttctgat gaatcctgga aacacagatg atacctacct gatgaacaat
     5881 gattacatcg tagttactcc ggttggtaaa gttgtagaga ttgcaggaga ggtcaagcgc
     5941 ccgttccgct atgaactgaa agaaggtgag aacctgatgg agttgctgga atacagcggt
     6001 ggactgaaag gaactgccta tacaagaaca gttaccatta aaagatacgc tgagaatgaa
     6061 aatcaggtca ttgatattaa tcttgatagc ctgcgtttac aaggtaaaga ttatgagttg
     6121 atagacggtg atatgatcac catagcaaag atcccggaag tggtggagaa tgtggtcagt
     6181 attaccggag ctgtgcgctt tcccggcact tatcagcttt ctgaaggatt gcggatatca
     6241 gatctggtag ataaagccaa agggcttaac tatgatgcct acctgactcg tgcttatctc
     6301 attcgcaaga atgaaaagat gaatgaggta tatattccat tcgaccttaa ggaggtgatg
     6361 gatgatccca attctgtttt caacttcgaa ctgaccaaat ttgatgtgat cgaagttttc
     6421 agcaaagacc gcttccgtga agtcttcaac gtctctatag aaggtgcagt aaaggctcct
     6481 ggtcagttct cctactacga caaaatgacc ttgaaagatc tgctttacta tgcaggtggt
     6541 ttgcgtgtgg aagcggcaaa caacaagata gagatctcac gtattgtgaa ctttaacgaa
     6601 gccaaggacc agaatgaacc aacccgggtg gtgatcgaaa gtatcagcat tggtaaggaa
     6661 ctggagctca cagacgaagc tgaaaatttc atcattcagc cttatgacca tgtctatatc
     6721 agaacaacac ctgagtttga acttcagaaa atggtgacag ttcagggtga ggtcatttat
     6781 cccggcaatt acaccttgtt gtctaaaaca gaaacagttg ctgatgtcat agaaagagcg
     6841 ggtggtgtta cccagtatgc ctatgtggaa ggtgccacca tcaaaagaaa caatgttacc
     6901 aatacgctgc tgttcctgga taaagccatg aaagatccgg agtccaagta caactatgta
     6961 ctccgtgatc tcgatgtcat cgtgataccc agacagggtg atctggtgag tctggaagga
     7021 gccattgagt ttcctttcga gatctcagaa gctgaccagg agaccggaca ggtcatggtg
     7081 ccatatgaca agggaaggac tgcaagattt ttcatcaaga aatacggtaa gaattttgat
     7141 gatgacgcag agcggggcaa gacttatgta gttcagccaa acggctatgt taaacgcacc
     7201 cacaatttcc tctggttcat acatttctat ccacatgtca aggtcaaggg aagcactgtt
     7261 gtggtgccct ataaagagaa ggaaaaggaa gagcctgaac aaacagatac agtagcagct
     7321 gaaccattcg actggaatgt attcatggct accatcagtg cctctatcct gagctttgcc
     7381 acgatatacg tgctgatcaa caactccaac agaaataact aaccggcgaa acagcaggag
     7441 caacatgcag tacgagcgtc cttacaatga tgagatcaat attggtgacc tgatcaccaa
     7501 actgggtgaa taccggaagt atctcgtaaa gaaatggtgg gtcattctgg tttctgccct
     7561 ggtggtgggt atggcattgc ggttctatat caactggagg cctgaacact atatttctca
     7621 tcaggatttc tcagtaaagg gaactgaggg agaatctacc tctactcttg gatctctggc
     7681 cagttcattc ggtttcggta tctctacagg tgctgagttc accaatgagt atttccttgc
     7741 catcatgcag tcacgccgct tgatcaagga aacattgctg caggagagga ccattcagct
     7801 ggacaagcag gaaccacgca cagattatct ggtgaatttt tacctggaca tgtatccaag
     7861 gtgggccaag aagaaaaagc tcaaaaaatt caggatggat catggaaata ttgattccct
     7921 gacgatctat gaagacagct gtttgtctat tatttatgat gagatcatag accacgacct
     7981 gacagttgac tatacggatg acctggacct caaccagctt gaattttcca gtatcaaccg
     8041 gcagttttca ctggactttg ctaattatct ggcaaacgca gcttcagatt tctatatcaa
     8101 cagccagatc gtagtggaag tggaaacggt cagcatgatc cagcaccgtg cagattccat
     8161 cagggctgtc atggaaagca aggaagatca gctggccgcc ttgcaggaca gaactgcatt
     8221 taccataaaa gccgccggtc tgatcaatca ggggcgtctg cttcgtgata ttgaactgct
     8281 gaatctcgag tatggcgaag tatttgcaca gctggaatta gccaagtttg accttagaaa
     8341 caagactccg cttgtcagcg tggttgattc accacgttat agcacgatca aggaaaaaga
     8401 gcagatcatg ctgttcatga tcataggttt tgtactgggt atagttattt ccagtatagt
     8461 tctggtgatc cggaagtaca cccgtgattc tgtagctgag gcaaaacaga agcaggagct
     8521 catcagacag tatgaagaac aacaggctgc tgtcttgctg gaacaaaaag aaaacagaga
     8581 aattgaataa cgcacaggcg taaagagaca gcaatgatct ttaactcctt tcagtttctt
     8641 gtttttctac cctgcgtaat attcctgtat ttcgctattc cttcaaaatg gaggtggtta
     8701 tttctgttgc tatccagcta ttatttctat atgagctgga agccggagta tgcattcctg
     8761 atcgtagcat ctactgctat cgattattat tcaggtatgc ggatgtcggc cattacagat
     8821 aagaagaaac gccgtccttt cctgatgcta agcatcttta ctaacctcag tttactgtta
     8881 ctctttaagt attttaattt tttcaatgag tctgccagag cagtctttga ttcctttaac
     8941 attttctaca atgttccgga attcaacatg ttcctccctg taggtatatc attttataca
     9001 tttcaaacgc tcagc
//
LOCUS       DAOMAX010000092         8988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1100_length_8988_cov_4.149110, whole genome shotgun sequence.
ACCESSION   DAOMAX010000092 DAOMAX010000000
VERSION     DAOMAX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1100_length_8988_cov_4.149110"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK742_06350"
     CDS             complement(<1..586)
                     /locus_tag="PK742_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HQU76245.1"
                     /translation="MKLRNILLVLFALLAFAPVKAQIGIFKKDKQEAPEDTLYHLFLD
                     SVIIEARNLRNYNYSRYEYVVRKVYPLADTAVALFHEVHEVTADMKKREDKKYRKQLE
                     KDLKENFEDRLKNMSRTEGLVLIKLIERKTGMSMYDLLRDVKNTRTAWWWQNLGKLYG
                     YDLREGYSPDQNPMLETIISEYEQKHNIPPPTPFK"
     gene            complement(592..1752)
                     /locus_tag="PK742_06355"
     CDS             complement(592..1752)
                     /locus_tag="PK742_06355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQU76246.1"
                     /translation="MKFLAAPLVLSLVLSGLLQSAKRLPVEELMPAAEDTVAADTLTH
                     SYTSISAIGDLMCHSTQYKYVATGDGHYDFTPCFSAIAPVLKMADLCVGNLETTLAGR
                     SRPYSGYPQFNTPDDYVVYLKEAGIDFLVTANNHSNDTGEKGIRRTLQVIDSVGFYHT
                     GTFGSEEERNAPQLLELNGIKVCILAYTYGTNGFELPAGKPWLVNQIDSALIRQDVRQ
                     ARAQGAEVIITFMHWGTEYSRKPDSYQEKVATWCVDAGADIILGSHPHVLQPAGRYIT
                     RNAAVDTGFIAWSMGNFISNQRDRYTYEGIVWHIGIEKELATGKIRIADLSYTPTWVY
                     RGTLPDHQVHIVYSARDTLPEWMPAGMWKELDNSALHTAALMENLNGIIFEP"
     gene            complement(1756..2985)
                     /locus_tag="PK742_06360"
     CDS             complement(1756..2985)
                     /locus_tag="PK742_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQU76247.1"
                     /translation="MIDKPAKSIASELASQAGFMVMEKMMEIEEKQSRLFIGIPKETS
                     FYENRVPLTPESVETLTSNGHRLIIESRAGEASRFSDKDYSEAGARIVYDKKEVYEAD
                     ILLKVAPPSAEDLELMHEGQTIISPLHLPTLKKEIITSLMSKRITALAFEYIVDESGT
                     FSFVRCMSEIAGSSAILIASELLATHKPGRGILLGGLSGISPTEILILGAGVVGEFAA
                     RAALGLGATVKVYDNNVYKLSRLQNNIGTRVYTSVINKKTLLKDIRTADVLVGAIHSE
                     LGRTPIIVSEAMVMEMKAGAVILDVSIDQGGCIETSEVTTHAHPTYIKHDVIHYCVPN
                     IPSRVPRTASYAISNIITSTLIKFADHGGMESMLLYNAGIRHGVYMYKGHLTNIYLGQ
                     RFELKSNDINLFLASSR"
     gene            complement(2982..3395)
                     /gene="tsaE"
                     /locus_tag="PK742_06365"
     CDS             complement(2982..3395)
                     /gene="tsaE"
                     /locus_tag="PK742_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQU76248.1"
                     /translation="MQLVAHTEADLDHVVTQLLDLYPGQRLWMLKGQLGAGKTALVRA
                     FCRHYGVTDEVSSPSFGIVNSYAGDHHVFHFDLYRLEDPSELREIGLEDYIDSGNPVF
                     IEWPEIIEAALEEEGFLPVRVIVMDNQDRIFNLGK"
     gene            complement(3435..4985)
                     /locus_tag="PK742_06370"
     CDS             complement(3435..4985)
                     /locus_tag="PK742_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HQU76249.1"
                     /translation="MSNIRILWADDEIEMLKPQILFLEEKGYEVVAVSNGQDAIDQCT
                     DPQVDLIFLDEMMPGLSGLETLEKVKEKRPNIPVVMITKNETENLMEEAIGSQISDYL
                     IKPVKSQQILLTVKKLTENKRLVNERTDMSYQKEFQNIFFSLQDSLSHDKWMELYQKL
                     IYWELQLDKAGGSGMKEVFQSQKNEANVEFCKFVDKQYLNWVGKTEKDSPTMSHNLLE
                     RHVFPLLEPGVPVFFILIDNLRFDQYRIIEPLISERFRKRSENTYYSILPTATQYARN
                     AIFAGLLPAEIQKRMPALWLNDEEEGGKNQHEEEFLKDNIVRHRLDIKYTYNKILNHQ
                     EGKTLQDNILNLMNNDLNVIVYNFVDMLSHARTEMEVLKELASDETAYRSITRSWFDH
                     SPLHDALKKLSGKKVRIVVTTDHGTIRVNSPSKVVADRNTTTNLRYKSGRNLQYNKKD
                     VLEIRKPEEAGLPKAHVSSSYIFAKEDSFFAYPNNYNYYVNYFRNTFQHGGISMEEMI
                     IPFAVFEG"
     gene            5040..6263
                     /locus_tag="PK742_06375"
     CDS             5040..6263
                     /locus_tag="PK742_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQU76250.1"
                     /translation="MNKRKILNDPVYGFITVPFEIIFDLIEHPLVQRLRRIQQLGLSN
                     LVYPGANHTRFHHALGAVHLMGKAISVLRSRGHDITEEEAEAATIAILLHDSGHGPFS
                     HALETTLVSQVTHEDISLRIMEELNGIFSGRLDLAIRIFQGQYHKQFLHHLVSSQLDV
                     DRLDYLIRDSYFTGVHEGVIGYDRLIEMMDVVNDELVIEEKGIYSVEKFIVSRRVMYW
                     QVYLHKTVVCAEQMLIKVLRRANYLVKNGVEIEATPALHYFLKHEVEKKDFTPGSEAF
                     RHFAMLDDTDIYVALKYWMNAEDKVLSFLSRSIINRKLFRIEMDKHPVSASRLKEITD
                     KVRKRFQIEDDFDMQYYVFHDSTSNSAYTLENKNIKIKRKQGQVEDVTKVSDHLHLAA
                     LADTVVKHYLCYPKP"
     gene            6268..7305
                     /gene="lpxD"
                     /locus_tag="PK742_06380"
     CDS             6268..7305
                     /gene="lpxD"
                     /locus_tag="PK742_06380"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQU76251.1"
                     /translation="MEISAFELCTLLGGTLEGDPDVKVSRPAKIEEGSQGDICFLSNE
                     KYLKFAYTTQASVLIIDEHLELDAGVKPTLIRVKNAYTSFSQVLDAWQKSKMRSVMNG
                     VEQHAYIHPSAMIGQNVHIAPFCFVGEGATVGDNTVLMAGAYIGNEVRVGQDCMLHPG
                     VHILYGCEIGNRCIIHAGAVIGSEGFGFAPQPDGTFIKVPQTGNVVIEDEVEVGANTT
                     IDRATMGSTFIRRGVKLDNLIQIAHNVEVGENTVIAAQTGISGSTRIGRNCMIGGQVG
                     IVGHIEIADGTRINAQSGVSKGIRKSGTAVTGSPAFDYSEAMRAQVVFRKLPELMERI
                     AELEKKLKESA"
     gene            7309..8712
                     /locus_tag="PK742_06385"
     CDS             7309..8712
                     /locus_tag="PK742_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HQU76252.1"
                     /translation="MSSNRQQTIRKAVTVAGVGLHTGQQVEMTFRPAPVNHGFKFKRV
                     DLDGTPVIDADADLVVDTARGTTIAKNNVRVSTIEHTLAALVGMGVDNCLIELNASET
                     PIMDGSSRVFVEAIDSVGVEEQEADREPIVIKENLSYVDPVRQTEMMLIPADDYQVTV
                     MIDFDSKVLGQQHASLHHLEEFKAEFASCRTFAFLHELEMLYDNNLIKGGDLNNAIVI
                     VDKEVTPEEMDKLKKMFHKDSVTVKKEGILNNLELHYVNEPARHKLLDVVGDLALAGR
                     PIQGKVIANRPGHPSNVEFAKVIKAYHKQRKQMLAPQYDPDNEPVIDINRINQMLPHR
                     YPMLLVDKVVEITDKYVVGIKNVTFNEAFFQGHFPNNPVMPGVLQIEALAQAGGIFVL
                     NQVPDPENYDTYFLKIDKVKFKRKVLPGDTLILKMELMQPIRRGICEMKATAYVGSTL
                     VTEGELTAQVVKRSAVV"
     gene            8709..>8988
                     /locus_tag="PK742_06390"
     CDS             8709..>8988
                     /locus_tag="PK742_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HQU76253.1"
                     /translation="MNQPLAYVHPQAEIADNVVIEPFAFISKNVVIGEGTWIGANVTI
                     MPGARIGKNCRIFPGAVISAIPQDLKYRGENATVIIGDNTTIRECVTVN"
BASE COUNT         2312 a         2080 c         2193 g         2403 t
ORIGIN      
        1 atttgaaagg ggttgggggc ggaatattgt gcttctgctc gtattcactg atgatggttt
       61 ccagcatcgg gttctgatcc ggcgaatacc cttcacgaag atcgtacccg taaagctttc
      121 ctaagttttg ccaccaccag gcggtgcgtg tgtttttaac atcacgaagc aggtcataca
      181 tgctcattcc ggtctttcgt tcaataagct tgatcagcac cagcccttct gtgcgactca
      241 tattctttag ccggtcttca aagttttctt tgagatcttt ctctagttgc ttccggtact
      301 ttttgtcttc cctcttcttc atgtcggcag taacctcatg cacctcatgg aacagagcta
      361 ccgcagtatc tgccagcgga tatacctttc tgaccacata ctcatacctg ctgtaattgt
      421 agttgcggag attgcgagct tctatgatca cggagtccag aaaaaggtga tacagggtat
      481 cttccggtgc ttcctgcttg tctttcttga aaatacctat ctgcgctttt acgggtgcaa
      541 aagcaagcag ggcaaacaag acaagtagta tgttccggag tttcataagg gtcagggttc
      601 aaaaataatg ccgttcaggt tttccatcaa tgctgctgta tgtaaagcgc tgttatccaa
      661 ttctttccac atgcctgccg gcatccattc aggcaaggtg tcacgggcag aatacacgat
      721 atgtacctga tggtcaggaa gggtgccgcg gtaaacccag gtaggtgtat aagacagatc
      781 tgctatgcga atctttcctg tagccagttc tttttcaatg cctatgtgcc atacaattcc
      841 ttcataggta taacggtctc gctggttgga tatgaaatta cccatagacc atgcaatgaa
      901 accggtatca actgcagcat tccgtgtaat atatctgccg gctggctgta gcacatgcgg
      961 atgactgccc agaataatat cagcaccggc atccacacac caggtagcca ccttctcctg
     1021 ataactgtcg ggttttctgc tgtattccgt accccagtgc atgaaagtaa tgatcacctc
     1081 tgcaccctgt gccctggctt gccgcacatc ctgccgtatc aaagccgaat ctatctgatt
     1141 taccagccat ggttttcctg caggcagttc aaatccgttc gtgccatagg tataggcaag
     1201 tatgcagacc ttgatgccat taagttccag aagttgcggt gcatttcttt cttcctccga
     1261 tccaaaggtt cctgtatgat aaaaacctac cgaatcgatc acctgcaggg tgcggcggat
     1321 gcctttttcg ccggtatcat tgctgtgatt attggcagta accaggaagt cgatgcccgc
     1381 ttctttcaga tagaccacat aatcatcagg tgtattgaac tgcggatacc ccgagtacgg
     1441 cctcgacctt cctgcaaggg tggtctccag atttcctaca cagagatcag ccattttcag
     1501 aaccggagca atggccgaga aacagggggt gaaatcgtaa tgaccatcgc cggttgcaac
     1561 atatttatat tgtgtgctgt gacacataag atctcctatg gctgaaatgc tggtgtaact
     1621 gtgggtcagt gtatcagcag caactgtatc ttccgcagca ggcatcaatt cttcaacagg
     1681 caggcgtttc gccgactgta acaggccgct cagtacaagc gacaggacca aaggagctgc
     1741 caggaatttc attgcttatc tgctgctggc caggaacagg ttgatgtcgt tagactttag
     1801 ttcaaaacgc tgcccgaggt aaatattggt cagatgacct ttatacatgt aaacgccatg
     1861 tctgatgcct gcattataca acagcatgct ttccatgcca ccgtgatcag caaatttgat
     1921 cagggtgctg gtgatgatgt tgctgatggc ataagaagct gtgcggggaa cacggctggg
     1981 aatattaggt acacaataat ggatcacatc gtgtttgata taggtggggt gtgcatgtgt
     2041 ggtcacttca ctggtctcga tacagcctcc ctggtcaata cttacatcaa gaatgacggc
     2101 gccggctttc atctccatga ccatagcttc actaacaatg atgggtgtgc ggcccagctc
     2161 actgtgaata gcacctacca gtacatctgc agtgcgaata tccttcaata gcgtcttttt
     2221 gttgatcacc gaggtgtaga cacgggtgcc gatattattt tgtaatcttg ataatttata
     2281 tacattgtta tcatatacct tgactgtagc tccaaggccc agggcagctc tggcagcaaa
     2341 ttcgccaacc actccggcgc ccagaatgag tatctcagta ggagagattc ctgacagccc
     2401 gcccagcagg atccctcgtc cgggtttatg ggttgccaac agctcactgg caatcagaat
     2461 ggcagagctt ccggcaattt cgctcataca cctgacaaaa gagaaggtgc cgctttcgtc
     2521 cacaatgtat tcaaatgcaa gtgccgtgat gcgtttgctc atcaggctgg taatgatctc
     2581 tttcttaagt gtgggcaggt gcagcggaga gatgatggtt tgaccttcgt gcatcagttc
     2641 caggtcttcg gccgagggag gggcaacttt cagtaaaata tcggcctcat acacctcttt
     2701 tttgtcatag acaattctgg caccggcctc tgagtagtcc ttatcagaaa aacgggaagc
     2761 ttctcctgcc cggctttcaa tgatcagacg gtgtccattg gatgtaaggg tttccacaga
     2821 ctccggggtc agcggaacac gattctcgta aaaactggtc tcctttggga tgccaatgaa
     2881 taagcggctc tgtttttctt ctatctccat cattttttcc atgaccatga accctgcctg
     2941 agaggccagt tctgaggcaa tggattttgc cggcttatca atcattttcc taaattaaag
     3001 attctgtcct gattatccat aacgatcact cgaaccggaa gaaacccttc ttcctcaagg
     3061 gcagcttcta tgatctccgg ccactcaata aataccgggt ttccgctgtc aatatagtct
     3121 tccagcccga tctcccgaag ttctgaaggg tcttccagac ggtacagatc aaagtgaaaa
     3181 acatgatgat ctcctgcata actgtttact atgccaaatg atggactgga aacctcgtct
     3241 gtaacaccgt aatgtctgca aaaggctctg acgagggcgg ttttcccggc tccaagctga
     3301 cctttgagca tccatagtcg ttggcccggg taaagatcca ggagctgtgt taccacatga
     3361 tccaggtcag cttctgtatg ggcaaccaat tgcatattgc aaagctatcc aaccatgctc
     3421 ttgcctgcaa ccattcagcc ctcaaagaca gcaaacggaa tgatcatttc ttccatactg
     3481 ataccgccgt gctggaaggt gtttcggaaa taattgacgt agtaattgta attgttcggg
     3541 taggcaaaaa agctatcttc ttttgcaaag atgtaagagg agctaacatg ggccttgggt
     3601 agtcctgctt cttcgggttt gcgtatctcc agcacatcct ttttgttgta ttgaaggttt
     3661 cggccgctct tgtaacgcag gttggtagta gtgttcctgt cagcaactac cttagacggg
     3721 ctgtttaccc tgatggtgcc atggtctgtg gtcaccacga tgcgcacttt cttgccactc
     3781 agttttttca aggcatcatg caagggcgaa tggtcaaacc atgatcgtgt gatggacctg
     3841 taagcggtct cgtcactggc cagctctttc aacacctcca tctctgtgcg tgcatgcgac
     3901 agcatgtcca caaagttgta aacgatcaca ttgagatcat tattcatcag gttgaggatg
     3961 ttgtcttgta aagtctttcc ctcctggtga ttaagtatct tgttgtaagt gtatttgata
     4021 tccaggcgat gtcttacaat gttgtctttc aggaattctt cttcgtgctg attcttgcct
     4081 ccttcttctt catcattcag ccacagtgca ggcatccgtt tctggatctc tgctggcagc
     4141 agtcccgcaa agatggcatt gcgggcatac tgcgtggccg taggaagaat gctgtaataa
     4201 gtgttttcag atctctttct gaagcgctca ctgatcaacg gctcaatgat ccggtactga
     4261 tcaaaacgga ggttgtcaat tagaatgaaa aaaaccggta cacccggttc caggagcggg
     4321 aatacatgtc gctccagcaa attgtgagac atggttgggc tgtctttttc tgttttaccc
     4381 acccagttga gatattgttt gtcaacaaac ttgcagaact ccacattggc ttcgttcttc
     4441 tgcgactgaa atacctcttt catacctgaa ccaccggctt tgtccagttg taattcccag
     4501 tatataagtt tctgatagag ctccatccat ttgtcatgac tcaggctgtc ctgaaggctg
     4561 aagaagatgt tctgaaattc cttctggtag ctcatgtccg ttcgctcatt gaccagtctt
     4621 ttgttctctg taagtttttt gactgtaagc aggatctgct gagatttgac cggcttgatg
     4681 aggtagtcac tgatctgtga accgatggct tcttccatca ggttttctgt ttcattcttg
     4741 gtaatcatca ccaccggtat gttcggtcgc ttttccttga ccttctccag cgtttccagt
     4801 ccggaaagcc cgggcatcat ttcatccagg aagatcagat ccacctgagg gtctgtgcac
     4861 tggtctattg catcctgtcc gttgcttact gccactactt cgtatccctt ttcttccagg
     4921 aacaggatct gtggtttcag catttctatc tcatcatcag cccaaaggat acgtatatta
     4981 ctcatgttat cagttacttt tgtgaagctt cggcaaagtt acaggttaaa cagccaggtt
     5041 tgaataagcg aaagattttg aacgaccctg tttacgggtt tattacggtt ccatttgaaa
     5101 ttattttcga cctcattgag catccgctgg tgcaaagact caggcgtatc cagcaattgg
     5161 ggttgagcaa cctcgtctat ccgggtgcca accataccag gtttcaccat gcactgggcg
     5221 ctgtgcatct gatgggtaaa gccatcagcg ttctgcgcag cagaggacat gatattacgg
     5281 aagaagaagc tgaggcggct accatcgcca ttctgctgca tgattcagga cacggcccgt
     5341 tctctcatgc gctggaaacc accctggttt ctcaggtaac tcatgaagat atcagccttc
     5401 gcatcatgga agagctgaac ggaattttca gtggccggct ggatctggcc atccgtattt
     5461 ttcagggcca gtatcataag cagttcctgc accatctggt aagcagtcag ctggacgttg
     5521 accggctgga ttatctcatt cgagacagct acttcacggg cgtacatgaa ggggtaatag
     5581 gatatgacag gcttattgaa atgatggatg tggtcaacga tgagctggtc atagaagaaa
     5641 aggggatcta ttctgtagag aaattcattg tgagcaggcg ggtcatgtac tggcaggttt
     5701 acctgcacaa gaccgtggta tgtgctgaac aaatgctgat caaggtactt cggcgggcta
     5761 attatctggt caagaacggg gtggaaattg aagctacacc agccttgcat tactttctga
     5821 aacacgaggt ggagaaaaag gattttacac ccggaagcga ggcgttccgg cattttgcta
     5881 tgctggatga cactgatatc tatgtagcat tgaaatactg gatgaacgca gaagacaaag
     5941 tgctttcttt cctttcgagg tctatcataa accggaaact gttccgcatt gagatggata
     6001 aacatccggt cagcgcatcc cgtcttaaag agatcaccga caaggtcaga aagcgttttc
     6061 aaatagagga tgattttgat atgcagtact atgtatttca cgacagcacc agcaacagtg
     6121 cctacacgct ggagaataaa aacatcaaga tcaaacgcaa gcaagggcag gtggaagatg
     6181 tgaccaaagt gtcagatcat ctgcatctgg ctgccctcgc tgatacggtc gtaaagcact
     6241 atctttgtta tccgaaaccc tgaccggatg gagatttctg cctttgaatt gtgtaccctt
     6301 ctgggaggca ccctggaggg tgatccggat gtaaaagttt cccgaccggc taaaattgaa
     6361 gagggaagcc agggtgatat ttgtttcctg agcaacgaaa agtatctcaa attcgcttac
     6421 accacacagg cttcggtgct gatcattgat gagcacctgg agttggatgc cggtgtgaag
     6481 cctacactca tccgggtgaa gaacgcctac accagttttt cgcaggtact ggatgcctgg
     6541 cagaaaagca agatgcgcag cgtcatgaac ggtgtggaac aacacgccta tattcaccca
     6601 agtgccatga tcggacagaa tgtgcacatt gctccgttct gttttgtggg agaaggtgca
     6661 acagtaggag ataatacagt actgatggcc ggtgcgtata tcggaaatga agtacgtgta
     6721 gggcaggatt gtatgctgca cccaggagta catattttgt atggatgtga gatcggtaac
     6781 agatgtatca tacatgcagg agctgtgatt ggcagcgaag gttttggttt tgcaccacaa
     6841 cctgacggta cattcatcaa agttccgcaa accggcaatg tggtgataga agatgaagtg
     6901 gaagttggag caaatacaac cattgaccgt gccacaatgg gttcaacctt catcagaaga
     6961 ggggttaaac tggataatct catacagatc gcacataatg tggaggttgg tgaaaacacg
     7021 gtcattgctg ctcagacagg catcagtggc agcacccgca tcggcagaaa ttgtatgatc
     7081 ggtggtcagg ttggcatagt aggccatatt gagattgccg atggaacccg catcaatgca
     7141 cagagtggtg tttcaaaagg tatcagaaaa tccggcaccg cggttacggg ctcaccggca
     7201 tttgattaca gcgaggccat gcgtgcgcag gtggtcttcc gaaagctgcc ggaactgatg
     7261 gaacgcatag cagaattgga aaaaaagctc aaagaatcag catagatcat gtccagtaac
     7321 agacagcaaa ctatcaggaa agcagtcact gtagcaggag tagggttgca tacaggtcag
     7381 caggtggaaa tgaccttccg gccggcacct gttaatcatg gatttaaatt taaacgggta
     7441 gatctggatg gcacccctgt tattgatgca gatgcagacc tcgtggtgga caccgccagg
     7501 ggaacgacca tcgcaaagaa taatgtcagg gtaagtacca ttgaacatac cctggcggca
     7561 ctggtaggaa tgggtgtaga taactgcctg atcgaactca atgccagcga aacacctatt
     7621 atggacggga gctccagggt gttcgtggaa gctatagatt ctgttggtgt ggaagagcag
     7681 gaagccgaca gagaacccat cgtcatcaaa gagaatctct cttatgttga tcctgtccgt
     7741 caaactgaaa tgatgctgat tccggccgat gattaccagg taacggtcat gatcgacttt
     7801 gattcaaaag tactcgggca gcagcacgct tcattacacc atctggaaga attcaaagca
     7861 gaatttgcgt cttgcagaac ttttgcattt ctccatgaac tggagatgtt atatgataac
     7921 aacctgatca aaggcggcga tctcaacaat gcgatcgtga tcgttgacaa ggaagtgact
     7981 ccggaagaaa tggacaagct aaagaagatg ttccataagg attccgttac cgtgaaaaag
     8041 gaaggtatcc tgaataatct ggaattacac tatgtcaatg aacccgcccg ccataaactg
     8101 cttgatgtag tgggcgatct ggcacttgcc ggcagaccta ttcagggaaa ggttattgcc
     8161 aacagaccgg gtcacccttc caatgtggag tttgccaagg tcatcaaagc atatcataaa
     8221 caacgtaagc aaatgctggc ccctcagtat gacccggata atgaacccgt catagacatt
     8281 aaccggatca accagatgct gccgcaccgg tatccgatgt tgctggtaga taaagtggtg
     8341 gaaataactg acaagtatgt ggtaggcatt aagaatgtga cctttaacga ggcattcttc
     8401 cagggtcact ttcccaataa tcctgtcatg cctggggtgt tgcagatcga ggcgctggca
     8461 caggcaggag gtatcttcgt actgaatcag gtacctgatc cggagaatta tgatacctat
     8521 tttctgaaaa ttgataaggt caaattcaaa cgcaaggtgc tgccgggaga tacattgatc
     8581 cttaaaatgg aactgatgca gcctattcgt cgtggcatat gtgaaatgaa ggctacagcc
     8641 tatgtgggca gtaccctggt aacagaaggt gaactgaccg cccaggtcgt aaaacgcagt
     8701 gctgtagtat gaaccaacca ttggcgtatg tacatccaca ggcagaaata gccgataatg
     8761 tggtcattga accgttcgct ttcatttcca agaatgtagt gataggagag ggtacctgga
     8821 ttggtgccaa cgtgaccatc atgcccggag ccagaattgg caagaactgc cggatctttc
     8881 cgggtgcagt catatctgca attccccagg atctgaagta ccgcggagag aatgctacag
     8941 tgatcattgg cgataatacc accatccgcg aatgcgtaac agttaacc
//
LOCUS       DAOMAX010000093         8942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1110_length_8942_cov_3.947226, whole genome shotgun sequence.
ACCESSION   DAOMAX010000093 DAOMAX010000000
VERSION     DAOMAX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_8942_cov_3.947226"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PK742_06395"
     CDS             complement(<1..1058)
                     /locus_tag="PK742_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQU76254.1"
                     /translation="MTVLVAALTTFGTMFALNRYFPAGNDTAAQTGSPFQLADYKFPV
                     APDGMAPVDFTFAAALTTPAVVHVKTSFTQTAAAGGQDIFRQFFGDDFNWDYGSRQAQ
                     ATGSGVIVSADGYIVTNYHVVKNADDIEVVLFDKQKMKATLVGTDPSTDVALLKVDGK
                     DMPTIQYGNSDSVRVGEWVLAVGNPFDLTSTVTAGIVSAKGRKIDILGENASTPVESF
                     IQTDAAVNPGNSGGALVNTEGRLIGINTAIATPTGTFAGYSFAVPVNIVQKVVKDLRK
                     YGTVQRAYLGVEIDITKDDLGGVRVASVVDGSGADAAGIKEGDIITHINKAPITSFPE
                     LQEQVSQYDPGDKVNVDLV"
     gene            1197..1994
                     /gene="dapF"
                     /locus_tag="PK742_06400"
     CDS             1197..1994
                     /gene="dapF"
                     /locus_tag="PK742_06400"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQU76255.1"
                     /translation="MEIPFHKYQGTGNDFVMIDNRDLSIPRDSVSLFAALCHRHLGVG
                     ADGVILLQEKEGFDFEMVYANADGRESTMCGNGGRCIVRFAEACGIVRERYHFLAVDG
                     MHEAMAYPDAVSLKMQHTAFPVRDGEAWVLNTGSPHWVRFTDGVGQLDMKKEGAAVRY
                     SDTYNKEGINVNFAEWLGDKLFMRTYERGVEDETLSCGTGVTAAALSLAAELHWPAGA
                     HRISVDTPGGTLRVHFTLEDDGSYRDIWLTGPARHVYSGTIRPAGLY"
     gene            complement(1988..2509)
                     /locus_tag="PK742_06405"
     CDS             complement(1988..2509)
                     /locus_tag="PK742_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76256.1"
                     /translation="MKRILLIIALLGGLSKANAQLEYIHSAGVGYYHSYLDKYGAIQY
                     WPRLNFLQMGWNGTLSLDTRLSLGYYVEQGTYAEEAYFTTLFPVTINWNMKNGNTRFA
                     TEQSGWYIGAGAALHEGWGTYSNYGPYATGGIRFDVGEAPFDINAGYMIDLSGNQSSQ
                     INISFNYMLNMYQ"
     gene            2689..3648
                     /locus_tag="PK742_06410"
     CDS             2689..3648
                     /locus_tag="PK742_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQU76257.1"
                     /translation="MIKYYKREQNNIVEIDRPEKDCWINVYPPFDHKRLAQLSDAIDI
                     PIDFLLDSIDINERSRFEVDDNVKLIVINTPIENDLEHSVDNEAFYITVPIGIILTKD
                     HNIIISSAQNRVIDWLFSVAIKHLDPADKDTVVLKIFEKNVFYFTQYLNEMNKRRYLI
                     EKELMHSSRNTELSKLLNIQKSLVYFVTDLRANELLMMKMARTNIVLGIKDDEEKSDY
                     LQDILIDSGQASEMANVYTNILNGTMDAFGSIISNNLNMVMKRLTSVTIILMVPTLIA
                     SFYGMNLSPLPLAGEGNAFWYILFISLVSAGVLYWVFRRIRWF"
     gene            3735..4544
                     /locus_tag="PK742_06415"
     CDS             3735..4544
                     /locus_tag="PK742_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76258.1"
                     /translation="MKNLITASLLLITHTVAFADVTLSERLEKPLYVVLSNDNVTINQ
                     ELREAFDNNWVLNEVNYITEDEYEPLSWTEENCFLIYQKNQQIDGEGNTVFSEVIRIV
                     AFTKKGKLVENIAGTPVFNSIEPHEADLVSSLRILQDKVRFELYREQGETEFSSYSEK
                     IQQSSGIVQLKKLYISAQDLEQGMDYASISDIYEGEVRIVDRSYIEQLIEQKASDAVY
                     VLIDHMQTSGISYLSTKTIMDAETGTVLYKDSDTALSPRGFGKRDFKKLAD"
     gene            complement(4597..5514)
                     /gene="fmt"
                     /locus_tag="PK742_06420"
     CDS             complement(4597..5514)
                     /gene="fmt"
                     /locus_tag="PK742_06420"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQU76259.1"
                     /translation="MPDHPRIIFMGTPDFAVPSLQAILDAGFPVPAVITAPDRPAGRG
                     LQMKASPVKAFAESKGIPVLQPEKLRDPEFLHTLLSYHADLQVVVAFRMLPEVVWSMP
                     PLGTLNLHASLLPDYRGAAPINHAILQGETETGVTTFFLQHAIDTGDIILQEKVAITP
                     DDTAGTLHDKLMQTGAQLVRKTVEAIATGAVQPVPQSGNSSKTAPKIFREDCEINWDQ
                     PVENIRNFVRGLSPYPTAWTTFQDKTLKVFSCSAEPSGSTKAAGSFETDGETYLRVAG
                     ADGWVYLTDIQPEGKKRMDIQAYLRGYRG"
     gene            complement(5514..6731)
                     /locus_tag="PK742_06425"
     CDS             complement(5514..6731)
                     /locus_tag="PK742_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQU76260.1"
                     /translation="MISQILLVLTIWSAAACKNSDSVEASGVVASGPEPAAIQVGAQR
                     TSEYYPLLAGKRVGVVVNQTSMIGNTLLPEALQGAGFEVTAIFAPEHGFRGEQEAGES
                     VSNGKDQKTGIPIVSIYGKRKKPTAADMKEVDIMIFDIQDVGARFYTYISTMTYVMEA
                     CAENNIPLIVLDRPNPNGFYVDGPVRRTGYESFVGIHPVPIVHGMTVGEYAKMVNGEG
                     WLSGGLTCELTVITCEGYDHKMKYDLPVPPSPNLKTMRSIYLYPSLCLFEGTNFSVGR
                     GTPDPFSVVGSPYVRGYRFSFVPSKKFSRSEPVFEDETCYGIDLRTMPIDSVADGRIH
                     LEYLMKMYKAFDPGQEFFRSDGFFDKLAGNDVLAEQLRARLSEEEIRLSWQDELIRFK
                     AIRKKYLLYPDFE"
     gene            6762..7997
                     /locus_tag="PK742_06430"
     CDS             6762..7997
                     /locus_tag="PK742_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU76261.1"
                     /translation="MKFTLFIARRVIRQENRSFSGLIIIIARLAIALSLAVMIVATAM
                     VSGFRETISDKIYGFWGHVNISRQSLRSSFDDLPMPVDSAFLNWAGQKPDIAAVQVYA
                     RKAGMLKTRTDLEGVIVKGVSSDFYSDSFRKYMVGGELPAFDGDQPAGDMVLSRSIAQ
                     RMQLEVGDSAVLYFIDQDESGLFNQRYRKLRIAGIYNTGLEEFDKMFVLADIRHIRRL
                     NDWGPQEVGGYEIFANRTDLILPVYEEVAREADPFWEVQSVYELIPGIFDWLNLQRIN
                     ERIIIILMLVVALINMVTSLLILILERTTMIGTLKALGANNRSVRSIFLVQAAYIIVW
                     GMVIGDAIGLSLCYLQQAFGIIRLPEESYYVSVAPVSIHYGSIILLNIATLAVSLLFL
                     LIPSALVSRLHPVKTLRFS"
     gene            complement(7896..8129)
                     /locus_tag="PK742_06435"
     CDS             complement(7896..8129)
                     /locus_tag="PK742_06435"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HQU76262.1"
                     /translation="MPELEEGRDYIIDTNGLLVLTAAYLLRRGYCCGNGCRNCPYDKV
                     SGKPKRFNRMQSGYQGTGDEQKQQTDSQGGDVE"
     gene            complement(8131..>8942)
                     /locus_tag="PK742_06440"
     CDS             complement(8131..>8942)
                     /locus_tag="PK742_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HQU76263.1"
                     /translation="IRVFIERLNGQRSLLNNIPFGAKFGGATGNFNAHFVAYPDVDWI
                     MFADQFVSNRLNLQRYRYTTQIEHYDNLSAAFDGLKRINVILLDLCKDLWTYISMDYF
                     RQKVNPNEVGSSAMPHKVNPIDFENAEGNLGIANALFEHMSSKLPVSRLQRDLTDSTV
                     LRNIGVPLAHTSIAFRSILKGLRKLEVNEDKITADLDRNWAVVAEAIQTILRREGYPQ
                     PYEALKALTRGKSSITREDMHAFIDSLDINAALKEQMKGISPFNYTGFRFF"
BASE COUNT         2267 a         2125 c         2209 g         2341 t
ORIGIN      
        1 accaggtcca cattgacctt atcgcccgga tcatactggc tcacctgctc ctgtaattca
       61 gggaaactgg tgatgggggc tttgttgatg tgggtaatga tatcgccttc cttgataccg
      121 gctgcatcag ctccggaacc atccaccaca ctggccacac gcactccgcc caggtcatct
      181 ttggtgatgt caatttccac acccagataa gcacgctgca cggtgccgta tttgcgcagg
      241 tctttgacaa ccttttgtac gatattgacc ggcacggcaa aactgtagcc tgcaaacgta
      301 ccagttggtg tggcgatggc agtattgata ccgatcaacc tgccttccgt attcaccagc
      361 gcacctccgc tgttacccgg gttgacagct gcatcggtct ggatgaaact ctctaccgga
      421 gtggaagcat tttctccaag aatatcgatc ttccggcctt tcgcactcac gatgccggct
      481 gtaacggtgg aggtcaggtc gaagggattt cccacagcca gcacccattc gcccacgcgg
      541 acagaatctg aattaccata ttgtatggtg ggcatgtcct taccatccac tttaagcaga
      601 gccacatctg tggaaggatc agtgccaacc agggtggctt tcattttttg tttatcgaac
      661 aacaccactt caatatcatc ggcattcttc accacatgat agttcgttac gatataacca
      721 tcagcactta ctatcacacc cgaaccggta gcctgtgcct gcctgctgcc ataatcccag
      781 ttgaaatcat caccaaaaaa ctgtcggaag atgtcttgtc cgcctgcagc agcagtctgt
      841 gtgaaagagg tctttacgtg caccacggca ggagtggtca gcgcagcagc gaaggtaaag
      901 tcaacaggtg ccataccatc cggagctacc ggaaatttat agtctgctaa ctggaatgga
      961 gaacccgtct gtgcagcggt atcgttgccg gcggggaaat acctgttcag ggcgaacatg
     1021 gttccgaagg tggtcagagc ggccaccagc acagtcatta cgagatgttt ggtcttcatc
     1081 atggttctac tcttttgtca gaataccagc aaattttatg ctcaaacgag atccttgcct
     1141 tctgccgtat cttcgcttaa caaactttaa cggcaaacgt accgtttcgg tttaatatgg
     1201 agataccatt tcataaatac cagggtacag gcaatgattt tgtcatgatt gacaacagag
     1261 acctgtctat tccgcgtgac agtgtgtcgc tttttgcagc tctgtgccac agacacctgg
     1321 gggtaggtgc agacggcgtc atactcctgc aggaaaaaga aggcttcgac tttgaaatgg
     1381 tctatgccaa tgctgatggc agagaaagta cgatgtgcgg taatggcgga cgatgcatcg
     1441 ttcggtttgc cgaagcatgt ggcatcgttc gtgagcgtta tcacttcctg gctgtggatg
     1501 gtatgcatga agctatggct tatccagatg cagtaagtct gaagatgcag catactgcat
     1561 ttcctgtgcg ggatggagag gcatgggtac tgaatacagg ttcaccacac tgggtgcggt
     1621 tcaccgatgg agtagggcaa ctggatatga aaaaggaagg tgctgcggtg cgatatagtg
     1681 atacatataa taaagaaggt atcaatgtca attttgccga atggctgggc gacaaacttt
     1741 tcatgcgcac ctatgaaaga ggtgtggaag acgagacctt atcatgcggt accggtgtaa
     1801 cggctgcggc tttgtcgctg gcagctgagc tccactggcc ggcgggagca cacaggatca
     1861 gcgtggatac accagggggg actctgcggg tgcactttac cctggaagat gatggaagtt
     1921 atcgggatat ctggctgacc ggtccggcca gacatgtgta cagcggaacc atacgccctg
     1981 ccggactcta ctgatacatg ttaagcatgt aattaaagga gatgttgatc tgactggact
     2041 ggttacccga caggtcgatc atgtatccgg cgttaatgtc aaacggagct tcgcctacat
     2101 caaaacgaat acccccggta gcatatggac cgtaattact gtaggtgccc cagccttcat
     2161 gaagggcagc tcccgcccct atataccacc cggattgttc agtggcaaaa cgggtgttac
     2221 catttttcat gttccagtta atggttaccg gaaagagggt ggtaaagtat gcttcttcag
     2281 cataggttcc ctgctccaca taataaccca gagaaaggcg ggtatccaga gacagggttc
     2341 cgttccatcc catctggagg aaattcagtc ttggccagta ttgaatggcg ccatacttat
     2401 ccagataact gtggtagtaa cctaccccgg cactgtgtat atattccagt tgcgcattag
     2461 ccttgctcag tccgcccaac agggctatga tcaggaggat tctcttcata cgtaaaggtt
     2521 gtgccgacat gtttgttacc tgccggtcaa acaaaaataa gcaggagtat tgacatagcc
     2581 tgacccggtg gcacgatgaa cgccccattg cactataaat tatacaggtt ttgaaaaaaa
     2641 caggctacat ttgcatgttc caaatccttc accgcttcat gggcagtcat gatcaaatac
     2701 tacaaaagag aacagaataa cattgtagaa attgacaggc ctgaaaaaga ctgctggatc
     2761 aacgtatatc cccccttcga ccataagcga cttgcgcagc tgagcgatgc catagacatt
     2821 cctatcgact tcctgctgga ctccattgat atcaacgaac gttcccgctt cgaggtggat
     2881 gacaatgtca agctgatcgt gatcaataca cctattgaaa atgacctgga acattcggtt
     2941 gacaatgagg cattttatat aacggtgcct attggtatca tcctgacgaa agatcacaac
     3001 atcatcatct ccagtgccca gaaccgggtc attgactggt tgttctcagt agccatcaaa
     3061 cacctggatc cggccgataa ggatacggtt gtgctgaaga tattcgagaa gaacgtattc
     3121 tattttactc aatatctgaa tgagatgaac aagcggcgtt atctcatcga aaaggaactg
     3181 atgcacagca gccgcaacac agaactttcc aagttgctga acatccagaa gtcgctggta
     3241 tattttgtaa cagaccttcg ggccaatgaa ctgctcatga tgaaaatggc caggaccaat
     3301 atcgtgctgg gcattaaaga cgatgaggag aaaagcgatt acctgcagga tattctcatt
     3361 gacagtggcc aggcttcaga aatggccaac gtatatacca atatccttaa cggcaccatg
     3421 gatgcttttg gctccattat atccaataac ctgaatatgg tgatgaaacg actcacctct
     3481 gtgaccatca tattaatggt acctaccctg atcgcttcct tctacggcat gaacctttcg
     3541 cctctaccgc ttgccggcga ggggaatgcc ttctggtata tccttttcat ttctctggtg
     3601 agtgcggggg tgctgtactg ggtattcaga agaatacgct ggttctgagg ggtgtggatt
     3661 tgtgaattcc ggaaatacat actttttttg tatctttaat gcaacttcag cggccttcag
     3721 gtcgttttac cattatgaag aacctgatca cagcatccct tctcctgatc acacacaccg
     3781 ttgcgtttgc agacgtgaca ctttcagaga ggctggaaaa gccactttat gtggtgcttt
     3841 ccaatgataa tgtgaccatc aatcaggagc tccgagaggc ttttgataac aactgggtac
     3901 tgaatgaggt caattatatc accgaagatg aatatgaacc tctcagctgg acagaagaaa
     3961 attgcttcct catttaccag aagaatcagc agatagacgg agagggcaat acggtctttt
     4021 cagaggtgat ccgtatcgtg gcttttacta aaaagggcaa actggtagag aatattgccg
     4081 gtaccccggt gttcaacagc attgaacccc atgaggctga cctggtcagt tccctgcgga
     4141 tcctgcagga taaagtgagg tttgagctgt accgggagca gggggaaaca gaattttcca
     4201 gctattcaga aaaaattcaa caaagttctg gtatagttca actgaaaaag ttgtacattt
     4261 cggctcagga tctggaacaa gggatggatt atgcatccat cagtgacatt tatgaaggtg
     4321 aggtccggat cgttgaccgt tcttatattg agcaattgat tgaacaaaaa gcgtcggatg
     4381 ctgtatatgt tttaatagac catatgcaaa cttccggaat ttcgtatctt agcaccaaga
     4441 ccatcatgga cgcggaaaca ggtacggttc tttacaaaga ttcagatacc gccctgtcgc
     4501 ccagagggtt tggaaaaaga gattttaaga aactggcaga ttgagggtta agcatttgtc
     4561 aggttttgca agaccgcctc tcaggcggtc ttttttttac cccctgtagc ctctcagata
     4621 agcttgtatg tccattcttt tcttgccttc aggctgaata tcggtcagat agacccagcc
     4681 atcagcgcct gctacccgca ggtaggtctc gccgtcagtt tcaaaactgc ctgcagcttt
     4741 tgttgatcct gatggctcag cagaacagct gaaaactttc agggtcttat cctgaaaagt
     4801 ggtccaggcg gtaggatagg gtgacaagcc gcgaacgaag ttgcgaatgt tctctaccgg
     4861 ctgatcccag ttgatctcac aatcttcacg aaatatctta ggtgctgtct tagagctgtt
     4921 gcccgattga ggaacaggct gaactgctcc ggtggcaatg gcttctaccg tttttcttac
     4981 cagttgtgcc cccgtttgca tgagtttatc atgcagggta ccggcagtat catccggggt
     5041 aatagccact ttttcctgca gaatgatatc gcctgtatct atagcgtgtt gcaggaagaa
     5101 tgtggtcact ccggtctctg tttcgccttg caggattgcg tggttgatgg gagcagcacc
     5161 gcgataatcg ggcaggagcg aagcatgcag attcagggtg cccagagggg gcatgctcca
     5221 gaccacttcc ggtaacatgc gaaaggctac cactacctgc agatcggcgt gataagaaag
     5281 aagagtatgc agaaattccg ggtctctcag tttctcgggc tgaagtaccg gaatgccttt
     5341 agattcggca aacgctttta ccggcgatgc tttcatctgc agaccgcggc cggctggcct
     5401 gtcaggcgca gtgatcaccg ctggaacggg aaaacctgca tcaagtatgg cctgcaggga
     5461 gggcacagca aaatctggcg tgcccatgaa aatgatgcgg ggatgatccg gcattattcg
     5521 aaatcaggat agagcagata tttttttctg atggccttga aacgtatcag ttcatcctgc
     5581 cagctcagcc tgatctcttc ttcgctcaaa cgtgcacgca gttgttctgc cagtacatca
     5641 ttccctgcca gtttgtcgaa gaatccgtca gaacgaaaga actcctgtcc ggggtcaaag
     5701 gccttgtaca ttttcatcag gtattccagg tggatgcgtc catcggcaac agaatctatg
     5761 ggcatggttc tcaggtcgat gccgtaacag gtctcatctt caaagaccgg ctctgatctg
     5821 ctgaatttct tactgggaac aaatgaaaaa cggtatccac gaacataagg ggagcccact
     5881 acagagaatg ggtcaggtgt gcctcttccc acagagaagt tggtaccttc aaacaggcag
     5941 agcgaaggat acagatagat gcttcgcatg gtcttcaggt ttggagaggg aggtaccgga
     6001 aggtcatatt tcatcttgtg gtcataacct tcgcaggtaa taacggtcag ctcgcaggtc
     6061 agtccgccgc tcagccaacc ttccccattc accatcttcg catattcacc aacggtcatc
     6121 ccatgaacta tcggcaccgg atgtatccct acaaaagact cataaccggt gcgtctaacc
     6181 ggaccatcca cataaaatcc gtttggattt ggtctgtcca gaacaataag aggaatattg
     6241 ttttctgcac aggcctccat gacataggtc atagtactga tgtaggtgta aaaacgggcg
     6301 cctacatcct ggatatcaaa gatcatgata tccacttcct tcatgtctgc agcggtaggc
     6361 ttcttgcgtt ttccatagat cgaaacgatc ggaatgcctg ttttctggtc ttttccgtta
     6421 gagacgctct ctccggcttc ctgctcaccc ctgaatccat gttcaggagc aaagatggcc
     6481 gtaacctcaa aacctgcacc ctgcaaagct tcaggaagca gggtattgcc tatcatggag
     6541 gtctggttaa ccactacccc gacacgcttt ccagccagca gggggtaata ttctgatgtt
     6601 ctttgtgcac ccacctgaat ggcagcgggc tcaggcccgg aagcaacaac tccgcttgct
     6661 tcaactgaat cgctattttt acacgcagct gcgctccaga tggtcagaac cagtaggatt
     6721 tgtgatatca tcttggatgc taaattacac gcaatcacgg aatgaaattc acgcttttta
     6781 ttgcacgaag ggttatccgt caggaaaata ggtcattttc agggctgatc atcatcatcg
     6841 cccgcctggc aatagccctg agtctggcgg ttatgatcgt ggcaactgcc atggtgagcg
     6901 gatttcgtga aaccatttct gacaagatct atgggttctg ggggcatgtg aatatttcca
     6961 ggcaaagcct tcgttcttct tttgacgatc tgcctatgcc ggtagattcg gcttttctaa
     7021 actgggcagg tcagaaacct gatattgccg ctgtacaggt ctatgccaga aaagccggga
     7081 tgctgaagac ccgaaccgac ctcgaagggg tgatcgtgaa aggcgtgagc agcgattttt
     7141 acagtgattc attccggaaa tatatggttg ggggtgaatt gcctgctttt gatggagatc
     7201 agcctgccgg cgatatggtc ttatcacgtt ctattgcgca gcgcatgcag ctggaagtgg
     7261 gtgatagtgc cgttttgtat ttcattgacc aggatgaatc cggtctcttc aaccaacgat
     7321 acaggaaact gcgtattgca ggtatttaca ataccggtct ggaagagttt gacaagatgt
     7381 ttgtgctggc agatatccgg catatcagaa ggctcaatga ctgggggccg caggaagttg
     7441 gtggctatga gatttttgcc aacaggacag acctgatcct gccggtctat gaagaggtgg
     7501 ccagagaagc tgatcctttc tgggaagtac aatcggtgta tgaattgatt ccgggaatat
     7561 tcgattggct caacctgcaa cgaatcaatg aaagaataat tattatttta atgttagtgg
     7621 tggcgctgat caatatggtc acttcgctgc tgatccttat actggagcgt accaccatga
     7681 tcggcacact gaaagcgctg ggtgcaaaca atcgttctgt cagaagtatt tttcttgttc
     7741 aggcagccta tatcatcgtc tggggcatgg ttatcggaga tgcgatcgga ctatccctgt
     7801 gctatctgca gcaggccttt ggtatcattc gcctgccgga agaatcctat tatgtgtctg
     7861 tggcacctgt atcgatacat tacggcagca tcatcttact caacatcgcc accctggctg
     7921 tcagtttgtt gtttctgctc atccccagtg ccctggtatc cagattgcat ccggttaaaa
     7981 cgcttaggtt ttcctgatac cttgtcataa ggacaattcc tgcagccgtt gccgcagcag
     8041 tatccgcgtc taagcaggta tgcagcggtc agtacgagta atccgttcgt atcaatgata
     8101 tagtctctgc cttcttccag ctcaggcatg tcagaagaaa cggaaaccgg tataattgaa
     8161 tgggctgatg cctttcatct gttctttcag tgcagcattg atatccaggc tgtcaatgaa
     8221 agcatgcatg tcttcacgag tgatgctgga ctttccacgg gtgagggcct tcagtgcttc
     8281 atatggctgc ggatatcctt cccgccgaag gatggtctga atggcttcgg caactacagc
     8341 ccagtttctg tccagatctg cagttatctt atcttcattg acctccagtt tccgcaggcc
     8401 ttttaatatg cttctgaaag cgatggacgt atgggccagg ggcactccga tgttgcgcaa
     8461 caccgtactg tctgtaaggt cacgctgtaa tctggatacg ggcaacttgg atgacatatg
     8521 ctcaaacaat gcattggcga tgcccaggtt tccttcagca ttctcaaaat caataggatt
     8581 taccttgtgt ggcatggcag atgagccaac ctcgtttgga ttgacctttt gtcggaaata
     8641 atccatagag atataggtcc ataagtcttt acaaagatca agcaggatca cattgatgcg
     8701 tttcagtccg tcaaaggcag ctgaaaggtt gtcataatgc tcgatctgag tagtgtaccg
     8761 gtatcgctgc aggttcagcc tgttgctgac aaactgatct gcgaacatga tccagtccac
     8821 atccggatag gccacaaaat gtgcgttgaa attacctgta gcaccaccga atttggcacc
     8881 gaaagggata ttgttgagca aactgcgctg tccgttcagc ctttcaatga aaaccctgat
     8941 ct
//
LOCUS       DAOMAX010000094         8903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1116_length_8903_cov_4.607143, whole genome shotgun sequence.
ACCESSION   DAOMAX010000094 DAOMAX010000000
VERSION     DAOMAX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1116_length_8903_cov_4.607143"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="rny"
                     /locus_tag="PK742_06445"
     CDS             complement(<1..1592)
                     /gene="rny"
                     /locus_tag="PK742_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQU76264.1"
                     /translation="MDIMSVLIGAGGGLLIGLLLGRLFFSKSSAELEQARKDAADMLS
                     AAQKDISLLKEKATTEAENLKKDRLLEAKEKFLQMKAEIETERVSKLGKLEEQELRMK
                     NREKQLAQERENLKKKENEANSVRENLNRQLELVSVKKTELEKAYSEHVAKLEQIAGL
                     TAEQAKTQLVETLKDEARNQAMAHVKEIMEESKLKANKEAKKIILQTIQRTAAEQTIE
                     NTVSVFNLDSDDQKGQIIGREGRNIRALEAATGIELIVDDTPETIIISGFDPVRREVA
                     RLSLQRLVADGRIHPARIEEVVAKTRKQMEEHIMEIGERTVIELGISGLHNELIRMVG
                     RMRFRSSYGQNLLMHSKETANLCATMAAEMGLNPKLAKRAGLLHDIGKVPEEESELSH
                     ALLGAKLCEKYGEHPAIVNAVGAHHDEIEMTYVISPIVQACDAISGARPGARREILES
                     YLKRIKEMENLAMAYDGVEKAFAIQAGRELRVIVEAEKVDDKKSEDLSFQIAQKIQDE
                     MQYPGHIKVTVIREKRSVSFAK"
     gene            complement(1658..1963)
                     /locus_tag="PK742_06450"
     CDS             complement(1658..1963)
                     /locus_tag="PK742_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQU76265.1"
                     /translation="MQDQDVIHINVTICDRPYRLKIKPQEEEAVRSAAREISEHVKDL
                     QGQFAGKDKQDYLAMYALTLAVGSQTNENRLSGLQDTILEALTDLDDHLQKSLQQHS"
     gene            complement(2041..2352)
                     /locus_tag="PK742_06455"
     CDS             complement(2041..2352)
                     /locus_tag="PK742_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76266.1"
                     /translation="MKEITDSLKSIRANADRLLRLLQASRQETALLKNQLDTLRAQVA
                     EKNQAIDQLRKEMDVLKTAQLIRLQDNEPAESTEDQEEVKRRINTYIKEIDSCIRRLE
                     A"
     gene            complement(2349..4772)
                     /gene="pheT"
                     /locus_tag="PK742_06460"
     CDS             complement(2349..4772)
                     /gene="pheT"
                     /locus_tag="PK742_06460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQU76267.1"
                     /translation="MRISWNWLKELLPASVSVDQAAELLTDIGLEVESVEVWRSVKGG
                     LEGLVTGEVLAVSPHPDADRLQVTQVSIGEEAPLQIVCGAPNVAKGQKVVVATVGTTI
                     HPVSGEAFTIRKAKIRGVSSEGMLCAEDEIGLGDGHDGILVLPAATQVGIALTEIIPV
                     LEDHLLEIGLTANHADAFSHYGVARELSAALAVRSMGTIHPDFPENSPLPDGKPAIVV
                     EVQNHSACPRYSGIVIRNIQVGPSPEHIQERLKAIGMRAVNNVVDVTNLVLHELGQPL
                     HAFDLDKISGQRIVVRNVADGTTFHGLDDKEYTLSAQDLMICDAEGAMCIGGVFGGKD
                     SGVSASTTSIFLESAFFSGAGIRRSESRLGLKTDASSRFAKGTDPEMTVAALGRALFL
                     LQQTSEAEPVGGLVDIYPEKISPFKVELRKARLRNIAGTDIPDETVELIFRHLDIQIV
                     SSDEQGWQTEVPVRKNDVTREIDLIEEVMRFYGYDHIPVPKAVRTPFSLSPKPDLERI
                     RLQACRFLAAQGFYEVFTNSISRSKYVDRWMPAHQDQVVRLLNSLNAELDSMRPTTIF
                     SHLEAAQYNVNRKQQDLRFFEFGKVFNRSGDSYTEVSTLSLLISGRREPENWNTSKEI
                     TTIFDMKACVQLLSNQWHISAEETAFEDHPLLEHAIALHKGQEVVGVYGEVKPAIRDA
                     FDIRYPVYFAEIFWIKWLDGRSEDLTMHTLPKYPEVRRDLALILEPEVSFDKVRSVTR
                     QTAGDLLQDVVLFDVYEGEKLQGKKSYAIGLTFRDDTKTLTDKEVEASVNRVIKALEK
                     ETGATIRST"
     gene            complement(4804..5658)
                     /locus_tag="PK742_06465"
     CDS             complement(4804..5658)
                     /locus_tag="PK742_06465"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HQU76268.1"
                     /translation="MNKINIIITSAILLFAACEEIGPAIDFTPVDETLIDTSYIELPA
                     EAPQTKVVLLEDFTGVQCVNCPDAHEATAELLATYPDQLVVVAEHNYFAGPYSDSYQD
                     LGTPEAEELDDYLGPAPAWPAGFVDRKDFGTGTLYTLFVSNYAVYVEDQLPLVPACNL
                     YLEATYDDASAKALVKVTIKYTSDVTSVNHLSVMILENGIVDLQSTNDGIDAGYVHDH
                     VLRDMLTPATGVLLNDELTAGRVFVKEFSISVPDTWDPDKLDIVAFVHNFSDTDKEVL
                     QAASSHLE"
     gene            complement(5660..7234)
                     /locus_tag="PK742_06470"
     CDS             complement(5660..7234)
                     /locus_tag="PK742_06470"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HQU76269.1"
                     /translation="MLRSFILIILLAVLPLLSVAQISGDLQLQNDYFQRDSAIGAFNT
                     PQFDNLKSSVDSWLNTYYKKEDWGFEAGLRLDIFNNSNILNPGTPYTAQGIGRWYVSK
                     KFEDMKLTGGYIYTQFGTGLTFRSYEERPLGIDNALVGLEVDYHLTENWTVKGVAGKI
                     KRQFDLYGPVVKGLVTEGVWQLGDQLTILPGASAVNRTLDQSNMDIIASTINSYDLED
                     RFVPMYNVYLGSAFSTINYKKFSLYAEYARKSHEAIRDADGQFEDADGSAIYSSLTYS
                     TKGFGITGQVRKVDNWVVRTSPNEILLNGIMNYLPSLTKQNSLRLTARYQAVAQELGE
                     LGYQANLTWSPAKGYTITANFSHISDPENELLFQEEYIDVEMRKKTYKLLVGLQLVKY
                     NQAVFEFKPNAALVEPITPFVEFTYKFDRKKSIRSEFQFQKNDHDFGSWVYGLVEFNI
                     APKWSFTASDMWNFDPLKTDEALHYPLIAAVFSHKVHRFSVSYVKQVEGIVCTGGVCR
                     YEPAFSGLKFGLITSF"
     gene            7429..7917
                     /locus_tag="PK742_06475"
     CDS             7429..7917
                     /locus_tag="PK742_06475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQU76270.1"
                     /translation="MKSLAFFALLTIGISAMAQESLPDLELYDIRGNKVNVTEFGQNG
                     KITVFNFWATWCTPCKKELNNIADLYEDWVADYNVEIVAVSIDDAKTRANVVSYADGQ
                     GWEYTVLLDQNKDLQRLLNGQTVPFTVIVDQQGNVVDKHSGYVEGDEYILEDKLIELS
                     GN"
     gene            8063..>8903
                     /locus_tag="PK742_06480"
     CDS             8063..>8903
                     /locus_tag="PK742_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76271.1"
                     /translation="MNSKAFRGFIVLSAILPVLNGCGWLNSKEPTTDEERIARLVEMD
                     HGAIPVAYFFRNPDQTAYQISPDGRFISYLGPYQSRLNIYIQHTGQQDTVRLTAATER
                     DITSYFWKNESTLIYLQDNAGDENYRIYSVDTSGTEKVLAEFRNVRINVVDELQQNPS
                     EILISMNKSNPALFEPYRLDINSGEITRLAENKDIQNPVTSWVADNRGILRAAVSVEE
                     GTTTHLLYREQEDMPFRSLISSNWKDMITPLFFDADNTHIIALSNLDRDKTALIRLNP
                     DKPD"
BASE COUNT         2122 a         2228 c         2068 g         2485 t
ORIGIN      
        1 tatttcgcga aggaaacact tcttttttcc cgtatcacgg tcactttgat atggccggga
       61 tactgcattt cgtcctggat cttctgggcg atctggaagc tcaggtcttc agatttctta
      121 tcgtccacct tttcggcttc aactattacc cgcagctcgc gaccggcctg gatggcaaat
      181 gctttctcca ctccgtcata ggccatggcc aggttctcca tttccttgat gcgcttcagg
      241 tagctttcca gtatctctct tcgggctccg ggacgggcac cgctgatggc atcgcaggcc
      301 tgtacgatgg gagagatcac ataggtcatc tcgatctcat catggtgggc tcctacagca
      361 ttcacaatgg caggatgttc accatatttt tcgcacagct tggctccgag aagggcatga
      421 gacaattctg attcttcttc cggcacttta ccgatatcat gcagcaaacc ggcacgtttg
      481 gccagcttgg ggttgagccc catctctgcc gccatggtgg cacacagatt ggcagtttcc
      541 ttggagtgca tgagcaggtt ctggccatag gaagaacgga atctcatacg acccaccatt
      601 ctgatgagtt cattatgcag acctgaaata cccagctcga taaccgttct ttcaccgatc
      661 tccatgatgt gctcttccat ctgcttgcgt gtcttggcca ccacttcctc aattcgggcc
      721 gggtggatac gaccatcggc caccagtcgc tgtaatgaca ggcgggccac ttctcggcgc
      781 accggatcaa aaccggagat gatgatggtt tctggtgtat catccacaat gagttctata
      841 ccggttgctg cttccagcgc acggatattc ctgccttccc gcccgatgat ctgacctttc
      901 tgatcgtcgc tgtccaggtt gaacacagaa acggtgttct caatggtctg ctcggctgca
      961 gtgcgctgta tggtctggag aatgatcttc ttggcttcct tgttcgcctt gagttttgat
     1021 tcttccatga tctctttgac atgggccatg gcctggttac gggcctcatc cttgagggtc
     1081 tctaccagtt gggtcttggc ttgctctgcc gtgagtcctg cgatctgttc cagcttggcc
     1141 acgtgctcac tgtaggcctt ttccagctct gttttcttga cactgaccag ttcgagttgt
     1201 ctgttcagat tttcgcgaac actgtttgct tcgttctctt ttttcttgag gttttctctt
     1261 tcctgcgcca gttgtttttc gcggttcttc atccgcagtt cctgttcttc cagcttgccc
     1321 agcttgctga ccctttcagt ctcgatctcg gctttcatct ggaggaattt ctctttggct
     1381 tccagcagcc ggtctttttt cagattctca gcttctgtag tagctttttc tttcagcaga
     1441 ctgatgtctt tctgtgcagc gctcagcata tcggcagcat ctttccgtgc ctgttccagt
     1501 tcagcagagc ttttgctgaa gaacagcctg cccagaagga gtccgatcag cagccccccg
     1561 ccagcaccta tcaatacgct cataatgtcc atacctgtgg ttttatagtg acaggctggt
     1621 gtaataaagg caggcgaacc gtgtgtcagg ttacctgtca gctgtgttgc tgcaggcttt
     1681 tttgcaaatg atcatccagg tctgtgagtg cttccagaat ggtatcctgt aatccgctta
     1741 ggcggttttc attggtttgt gaaccaactg ccagtgtaag ggcatacatg gccagataat
     1801 cctgtttatc cttgcctgca aattgtccct gcaggtcttt cacatgctct gatatttctc
     1861 ttgcggcact gcgaacggcc tcttcctcct gcggtttgat cttcagccgg tagggtcggt
     1921 cgcagatagt aacatttata tggatgacat cctgatcttg cacaatccgg ttttaccttc
     1981 agatacacca gtacctaatc ttccaaaaat acagaaattc cggatatgca aatgccctgt
     2041 tcaggcttca agtctcctta tacaggagtc aatctccttg atataggtgt tgattcgacg
     2101 ctttacttct tcctgatctt cagtactttc tgcaggttca ttgtcctgca gcctgatcaa
     2161 ctgcgcagtc ttgagcacat ccatttcttt ccgcagctga tcaatggcct gattcttctc
     2221 tgctacttgt gcacgaaggg tgtccagttg gttcttcagc aaagcggttt cctgacggct
     2281 ggcctgcagc agcctgagca gccggtctgc attggcacgt atggatttca ggctgtctgt
     2341 gatctctttc atgtgctgcg tatggtggct cctgtttctt tttccagtgc tttgatgacc
     2401 cggttcacac ttgcttccac ttctttgtct gtaagggttt tggtatcatc acggaaggtc
     2461 aggccaatgg catatgactt tttgccctgc agtttctccc cttcatatac gtcaaagagc
     2521 acaacatcct gcaacagatc gcctgctgtt tgccgtgtca cggaccggac cttgtcaaac
     2581 gatacttccg gttccaggat gagtgccaga tctcttctaa cttcaggata ctttggtaag
     2641 gtatgcatgg tcaggtcttc actgcggcca tccagccatt tgatccagaa gatctcagca
     2701 aaataaaccg gatagcgaat gtcaaaagca tctctgattg ccggttttac ctctccgtac
     2761 acacctacta cctcctgccc tttatgcaaa gcgatggcat gttccagtag cggatgatct
     2821 tcaaatgctg tttcttctgc tgatatatgc cactgatttg aaagcaattg aacacatgct
     2881 ttcatatcga aaatagtagt tatttctttg gatgtattcc agttctccgg ttccctgcgc
     2941 ccgctgatca acaagctcag ggtggatact tcggtgtaac tatcgccgct ccggttgaac
     3001 accttgccga attcgaagaa gcgaaggtcc tgttgttttc ggttgacatt atactgagca
     3061 gcctccagat gactgaatat ggtggtaggc cgcatgctgt ccagttctgc attgagtgaa
     3121 ttcagcagtc ttacaacctg atcctgatga gcaggcatcc agcggtccac atattttgat
     3181 cgggaaatgc tgttggtaaa tacttcataa aatccctggg cagccagaaa tctgcaggcc
     3241 tgcagtctga tcctttccag atcaggttta gggctcaacg aaaaaggtgt acgaacagcc
     3301 ttgggaacag gaatatgatc ataaccgtaa aaccgcatga cctcttctat cagatcgatc
     3361 tcacgtgtta cgtcgttttt tctgacaggt acttccgttt gccaaccttg ttcatctgag
     3421 ctgactattt ggatgtcaag atgccggaat atcagctcaa ccgtctcgtc tggtatatct
     3481 gttccggcaa tattcctgag ccgggctttt ctaagttcaa ctttaaacgg agagatcttt
     3541 tccggataga tgtcaaccag gcctccaacg ggttctgctt cgcttgtctg ctgaagcagg
     3601 aacaaagctc ttcccagggc agctacggtc atctccggat cggttccttt agcgaatctg
     3661 gacgatgcat ctgttttcag tccaagcctt gattctgaac gacgaattcc ggcgccactg
     3721 aaaaaagcac tttccagaaa gatactggta gtggatgcgg atacaccgct gtccttaccc
     3781 ccgaacaccc cgccaataca catggcaccc tcggcatcac agatcatcag atcctgtgct
     3841 gataaggtat attccttgtc atccagacca tgaaatgtgg tcccatcggc tacatttctg
     3901 accactatac gttgcccgct gatcttgtcc agatcaaatg catgcagtgg ttgcccaagt
     3961 tcgtgcagca cgagattggt cacgtccact acattgttga cagcacgcat acctatagct
     4021 ttcagtcttt cctgaatgtg ctcaggagat gggccaacct gaatgttgcg gataacgatg
     4081 ccgctgtatc tcgggcaggc agaatgattc tgcacttcta ctactatagc gggctttccg
     4141 tcaggcaaag gactattttc aggaaaatca ggatggatgg ttcccattga tcttacagca
     4201 agtgcagcgc ttagttcacg ggctactcca taatggctga aggcatctgc atgattggca
     4261 gtaagtccta tttccagcag gtgatcttcc aataccggaa tgatctcagt aagagcaata
     4321 ccaacctgtg ttgcagcggg taagaccagg ataccgtcat ggccatcgcc cagcccgatc
     4381 tcatcttcgg cacagagcat gccttcactg ctcactcccc gaatcttggc ttttctgatg
     4441 gtaaacgctt ctcctgaaac cggatggatg gtggtaccta cagtggctac taccaccttt
     4501 tgtcccttgg caacattagg agcaccgcaa acgatctgca ggggagcttc ttcgccgata
     4561 ctgacctggg taacctgtaa acggtcagca tcggggtgag ggctcacagc cagcacctct
     4621 ccggtaacga gcccttccaa tcctcctttt actgaccgcc acacctccac actttccact
     4681 tcgagaccga tgtcggtcag cagttcagcg gcctgatcta cagagacaga tgcaggcaat
     4741 aattctttca gccagttcca ggatatcctc attaccggca aagttaatga ttcaggtatt
     4801 tcgctactcc aggtgtgaag aggctgcctg gagcacctct ttatcggtat cactgaaatt
     4861 gtgcacaaag gccacaatgt ccagtttatc cggatcccag gtgtcaggaa cggagatact
     4921 gaactccttc acgaaaaccc tgcctgcggt cagttcgtca ttcagcagca caccggttgc
     4981 aggggtaagc atatctctca acacatgatc atgcacatag ccggcgtcta taccatcatt
     5041 ggttgactga agatcaacaa tgccgttttc caggatcatc acactcaggt gattcactga
     5101 ggtcacatca ctggtgtatt tgatcgtcac cttgaccagt gcctttgctg aggcatcatc
     5161 atatgttgct tccaggtaca gattgcaggc cggaaccagc ggcaactgat cttccacata
     5221 cactgcataa ttggatacaa acaaggtgta cagggtgcct gtgccaaagt ctttacggtc
     5281 aacaaagccg gcaggccagg caggggcagg acccagatag tcatcaagct cttctgcttc
     5341 aggggtaccc agatcctggt agctgtcaga gtatgggccg gcaaaataat tgtgttcagc
     5401 taccactacc agctgatccg gataggtggc aagtagttct gctgtagctt catgggcatc
     5461 agggcagttt acacactgaa cacctgtaaa atcttccagc aataccacct tcgtttgggg
     5521 ggcctctgca ggtagttcta tgtaggatgt atctatcaga gtttcatcga caggagtgaa
     5581 atcaatagcc ggcccgatct cttcacaggc agcaaagagc agtatggcgg aggttattat
     5641 tatgtttatt ttgttcatat cagaatgatg taattagtcc gaatttaagt ccgctgaaag
     5701 caggctcata ccggcaaaca ccaccggtac aaactattcc ttcaacctgc ttcacataac
     5761 ttactgagaa acggtgaact ttatgagaga aaacggcagc aatgagcgga tagtgcaaag
     5821 cttcatctgt ttttaacggg tcgaaattcc acatgtcact ggctgtgaaa gaccatttag
     5881 gagcaatgtt gaattcaaca agaccataca cccagctgcc aaaatcatgg tcgttcttct
     5941 gaaactgaaa ctctgagcgg atggattttt tcctgtcgaa tttgtaggtg aactccacaa
     6001 atggagtgat gggttctacc agtgcagcat ttggtttgaa ttcaaacact gcctgattgt
     6061 attttaccag ttgcaatcct accagcagct tgtaggtctt cttgcgcatt tccacatcaa
     6121 tgtactcttc ctgaaagagg agctcatttt cagggtctga aatatgtgaa aagttggctg
     6181 tgatggtgta accttttgcc ggcgaccagg tgaggttggc ctgatagccc agttctccca
     6241 gttcctgtgc aacagcctga taacgggctg tgagccgcag cgaattctgt ttggtcagtg
     6301 atggcaggta gttcatgatc ccgttcagca ggatctcatt gggcgatgtg cgcactaccc
     6361 agttatccac tttgcgcacc tgacccgtta ttccgaagcc ttttgtactg taagtaagag
     6421 aagagtatat cgcacttcca tcagcatctt cgaactgccc gtcggcatct ctgatggctt
     6481 catggctttt gcgggcatat tcagcataca aactgaactt cttgtaattg atggtagaaa
     6541 aagctgaccc cagataaaca ttgtacatgg gtacaaatcg gtcttccaga tcatagctgt
     6601 tgatcgtgga tgcaatgata tccatgtttg actgatccag ggtgcgatta accgcagaag
     6661 cccctggcag aatggtcagc tgatcaccca gctgccatac cccttccgtg accaggcctt
     6721 ttaccacagg cccatacaga tcgaactgtc gtttgatctt tccggctacc cctttgaccg
     6781 tccagttttc ggtgagatga tagtccactt ccagaccaac cagtgcattg tctatgccca
     6841 atggtcgttc ttcataagag cggaatgtca aacccgtacc aaactgggta tagatgtacc
     6901 cgccggtcag cttcatgtct tcaaattttt tactgacgta ccatcggcca atgccctggg
     6961 ccgtataggg ggtaccaggg ttcagaatat tggaattatt gaagatgtcc agtcgcaaac
     7021 cggcttcaaa accccagtct tcttttttat aataggtatt cagccaggaa tctacagatg
     7081 acttcaggtt atcaaactgt ggggtattaa aagcgccaat ggcagaatcc ctctggaaat
     7141 aatcgttttg aagttgaagg tcaccgctga tctgcgccac ggaaagcaag ggaagaacag
     7201 ccagtagaat gatcagaata aagcttctca gcatggtcat aggtttcaat cgcaaattta
     7261 ggattttcag cgtcttgcag cccggaccaa acggctcggc aaacctgagg atgccatttt
     7321 catgtcatat tgaactgctg acctgcatat gggcagacct gacagtatgt tccatggcat
     7381 gaatcttgct gattacaggt acatttgtac cagtatagat ccaaccatat gaagtcactc
     7441 gcatttttcg ctttattgac cataggcatc tcagccatgg cgcaggaatc tctgcctgat
     7501 ctggaacttt atgacattcg gggtaacaag gtgaatgtta ctgaattcgg gcagaacggc
     7561 aagatcaccg tttttaattt ctgggcaacc tggtgtacgc catgtaaaaa ggaactcaac
     7621 aatattgcag atctgtatga agactgggtg gctgattaca atgtagagat cgttgcggta
     7681 tccattgatg atgcgaagac aagagctaat gtggtttctt acgcagatgg ccagggttgg
     7741 gaatacactg tcctgcttga tcagaataaa gacctgcaac ggttgctgaa cggacagact
     7801 gttccattta ccgtgatcgt agatcagcag ggtaatgtgg ttgataagca cagtggctat
     7861 gtagaaggag atgaatatat tctggaagat aaactcattg aactgagcgg aaactaaact
     7921 tcgttctcac ctgatcagaa gcccgccaca ggcgggcttc tttgttaaat gctgttaaac
     7981 agcgttgcgg gctgaaaccc tttgcctgtg ttgcctcgtt tcttgttata tatttgacgg
     8041 tcctgaagga ggagtgcagg tcatgaacag taaggcattt agaggattca ttgttttgtc
     8101 agccatatta cccgtgttga acggttgtgg gtggctgaac agcaaggaac caacaacaga
     8161 cgaagaacgc atagccagac tggtggaaat ggatcatggt gccattcctg tagcctattt
     8221 ctttagaaat cctgaccaga cagcctacca gatctctccc gacgggcgtt ttatctctta
     8281 tctgggcccc tatcaatccc ggctcaatat ctacattcag cataccggac agcaagacac
     8341 agtacggctg acagccgcta ctgaaagaga tattaccagc tatttctgga agaatgaaag
     8401 taccctgatc tatctgcagg ataatgccgg agatgagaac taccggatct attcagtaga
     8461 tacttctggt acagaaaagg tactggcaga attcagaaat gttcgtatca atgtggtcga
     8521 tgagctgcag cagaatccca gtgagatcct gatctccatg aataaaagta atccggcatt
     8581 gtttgaacca tatcgcctgg atattaatag cggagagata accagacttg ctgagaataa
     8641 agatattcag aacccggtta ccagctgggt ggcagataat cgtggaatcc ttcgggctgc
     8701 tgtttcagta gaggaaggta ccaccacaca cctgttgtac agagagcagg aagacatgcc
     8761 cttcagatcg ctcatttctt ccaattggaa agatatgatc acccccttgt tctttgatgc
     8821 agacaataca catatcatag ccttgtctaa tcttgatcgc gataaaacag cacttatccg
     8881 actgaatcct gacaaacctg atc
//
LOCUS       DAOMAX010000095         8897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1118_length_8897_cov_4.145216, whole genome shotgun sequence.
ACCESSION   DAOMAX010000095 DAOMAX010000000
VERSION     DAOMAX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1118_length_8897_cov_4.145216"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK742_06485"
     CDS             <1..188
                     /locus_tag="PK742_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76272.1"
                     /translation="SGEAVLVMQDISGNNLLKMNLLYTVGKNISELDLRSFPNGLYLL
                     QIQTSSGSVTQKIEVSR"
     gene            350..1081
                     /locus_tag="PK742_06490"
     CDS             350..1081
                     /locus_tag="PK742_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76273.1"
                     /translation="MKHRICIAFLLLSFSVSSVFAQVVTREKALATNVINAMGGTTAF
                     RNVPYISWQFFEKRTITWDKWNGDVRIDYLDQPLTIIVNLRADKVMVARDGKRMTDTD
                     SLAYYLDKGRTIWMNDSYWLFMPFKLLDKGVILHYMGKGETVDGKETEVLELTFRQVG
                     ATPLNKYHIHIDPGTYDVLQWDYYIRTDEEQPTIITPWTDYKWYGKIRLSSGRGSFGK
                     ITNIKLPDSLPAFTFRDPVKPLQGF"
     gene            1259..3232
                     /locus_tag="PK742_06495"
     CDS             1259..3232
                     /locus_tag="PK742_06495"
                     /inference="COORDINATES: protein motif:HMM:NF038117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HQU76274.1"
                     /translation="MKRTNLWLRMAGAALVLAMTAQSLKAQNAFIDLQPAGRYETMTF
                     GESATEIAAYDPQSQRLFSTNAFTGQVDVLDISDPEAPALLFSIDLSAYGSGANSVAF
                     RSGFLACAVEADVKTDNGSVVFFDADGNYVNSLEVGALPDMITITPNGDFALTANEGE
                     PSDDYLTDPLGTISIIDLRAGIPALTADDVTTLDFSAFNDAVLDPSIKINGPGASVAM
                     DLEPEYIAVSSNSKFAYVTIQEANAVAIVQIESPAIVGLVGLGFKDHMLPGNGLDASD
                     KADNIDIVNWPVKGLFMPDAIGFLRAGSGAYLITANEGDSRDYDAFSEEDRIKDVILD
                     TVAFPNYAGLQNDDSIGRLKIINTMGDIDGDGDYDELYTYGARSFSVWDLAGNLVYDS
                     GDELEQLTAAWYPDNFNASNDNQDLKNRSDDKGPEPEVLAIGRIFKNPYLFLGLERMG
                     EIMVYDMSDPASPSFVTHTNSRDFSVDPEDDASGDYGPEGLLFIPATQSPNGRNLLVT
                     SNEVSGSIAIYYTDYACGASKVSVCYDGVSYCLPITVAEALVTLGGTPGPCDGMRSML
                     GEEDLALGINAYPNPASDHVNIVLNDLPAGNWNLRLFDITGRMVFTGNVENDGSNWVT
                     YPVDVQQMPAGTYTCQVTSNNGVAFSTRIAVVQ"
     gene            complement(3290..5425)
                     /gene="rnr"
                     /locus_tag="PK742_06500"
     CDS             complement(3290..5425)
                     /gene="rnr"
                     /locus_tag="PK742_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQU76275.1"
                     /translation="MAKKKKINKPTGGRSGNLAKDLLEFIGERPAKGQSLKNILKWSV
                     KQAEGAGIFDAMEDLLYAQQIEEYQQDKFRLLQKDSGGSSVIGVVDITRSGNAYVLID
                     GRLNDVFVAARNTNRAFNGDTVRVRLRAGKKGKPEGEIVEVIKRARDTFIGHLRMNDG
                     FAFVETDKAFNNYDFFIAPEEVRRSKAQDGERVIVRLKDWPPAMKSPIGQIVSRLQPA
                     GDHESDMQAIMVETGIQYVFPKEVDQEAEKISNDIPEEEIRLRRDMRSTWTITIDPHD
                     AKDFDDAISLKQLDNGRWEVGVHIADVSHYVTPGTAIDKEAYSRATSVYLVDRVIPML
                     PEKLSNNICSLVPHQDRLVFSAIFEMDEEGKVHKEWFGKSVIHSDRRFTYEEAQEVIE
                     DRLDEYKAEIHTLNKIALALRNERFRKGAVAFETQEVKFILDENKKPVGLYVKERKAA
                     HMLIEDLMLLANRKVAEFMSKIQPPVPFVFRVHDFPDMDRLEEFSMMARRFGYKVRFD
                     QPNQVSARMNELMKEIQGKPEQNVLETMAIRCMAKAVYTTNNIGHYGLAFPHYTHFTS
                     PIRRYPDLMVHRSLFHQLHNMPRWASKEELEEKCRHSSAMERKAMEAERESVKYKQVE
                     FLMGHVGEEFDGVISGIQHYGIFVELVQNKCEGLVRTDSVRDELIYDEAKRELRSMTG
                     GRTFRMGETVRIKVQKADLDARQLDFVLA"
     gene            complement(5427..5975)
                     /locus_tag="PK742_06505"
     CDS             complement(5427..5975)
                     /locus_tag="PK742_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76276.1"
                     /translation="MFEQLLEIVRQQADEQIVRNDAIDNKFNEDAIQEAANSIQDTLR
                     DQLANGNLQDVMQLFRQGDAGADNPMVQQISSMFSKQLGSKFQVDGLKADGIASQLIP
                     MILSRFIKKTNDPDDASVDINDIFGSLTGNKSSGIDFSKILKQEKDDDGFGMDDIMDM
                     VKKGASSQKGGGGLLGSLLGGK"
     gene            6087..6581
                     /locus_tag="PK742_06510"
     CDS             6087..6581
                     /locus_tag="PK742_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQU76277.1"
                     /translation="MKSLYIIRHAKSSWEYSELHDLERPLTDRGKRDAVIMGTLLRKQ
                     NVHPGIVISSPALRAISTCRIICDEIGYDKSRIEINTSLYFKDVEEILAVIHSRGSRH
                     DDMFLFGHNPNFSNLAHTFSPEFDEIIPTCGIVCIRFEAESWGLAEAGSGKLEYFDMP
                     KNYR"
     gene            6768..7064
                     /locus_tag="PK742_06515"
     CDS             6768..7064
                     /locus_tag="PK742_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HQU76278.1"
                     /translation="MASYIAEIRLLNAGSDAFMQCIPRQRMMVNDMMAEGLITSYAVS
                     ADRKKMWCVVEADSVDEATRIIDQFPLSPFMEAEVKELLFHHMHGSLMGTISLN"
     gene            7087..>8897
                     /locus_tag="PK742_06520"
     CDS             7087..>8897
                     /locus_tag="PK742_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76279.1"
                     /translation="MSGRLLTILLILAGSSFLLTDMHHSGMTQEDTPENELAEAYDAF
                     AIDLLKKQDPETGRIPDGALMSAYRTLKARGYYRHEQGNTRSEDGWQQVNDYFPSLAI
                     TKITYDPNNTNVFYFCTGEGWFNADAVRGAGIFKSTDGGETWFQLPSTDSAIFQYCQD
                     IDVHPITGDIYVATRSGGLQRSTNGGESWQKVLGSGAGSGRNSICDVEITSDGGVFVG
                     IGIFETDGIYYSANGDSATFSKQTVGLPSTGYYRIELAVSKSDPDIAYAIPCSTSFRI
                     QGIYRTDDRGATWQATNLPGDDNELAARQAWYDLVIEVSPEDPNLIIAGGLNIWRSTD
                     GGGTWDQLTRGSLDSSLLRYMHVDQHAAVFRNQEEVYFGNDGGIYKTLNFSADNPTIY
                     SRNDGYNVTQYYAHAMSGQAGSDVLIGGTQDNGSHLAYNPGLSSFKPVSGADGAFCAI
                     NSINEDIFYTTTQYRRLFRFDNGGFEMPDTLTNPNVTDDNLLFINPIEIDPVDPENLY
                     MASNAGLWRLINASTADTSGWEKACTYGGTISAIAVSTNPPNLVFIGRNVSNAEPAVL
                     YDASFVDGGSSPFGLDPSNMIPDATGFGSTRYCSSIAV"
BASE COUNT         2185 a         2209 c         2133 g         2370 t
ORIGIN      
        1 agtcaggcga ggcagtgctc gtgatgcagg atataagtgg taataatttg ttgaaaatga
       61 acctgttata tactgttggt aaaaatattt cagagctgga tttgaggtca tttccaaacg
      121 gtttatattt gcttcagatt caaacatcct caggttctgt aacacagaag atagaggttt
      181 ccagataaat ctggtttttg ccattggaaa cctgttttgg ttgaaaaccc cggctgcaaa
      241 ccggggtttt ttcttgcaaa ctgatgagga aggtggacgg cttgtgatag gaggaaccaa
      301 accggatcac acatagtatt tgtaagaaac agtctaactg agtccggaaa tgaagcatcg
      361 catttgtatc gccttcctgt tgttatcctt ttcagtttct tccgtttttg cccaggtggt
      421 cacccgtgaa aaagcacttg ctaccaatgt cattaatgcc atgggtggaa ccactgcgtt
      481 tcgcaatgtg ccctacatca gctggcaatt cttcgagaag cgcaccatca cctgggataa
      541 atggaacgga gatgtcagga tcgactacct cgatcaaccg ctcaccatca tcgtcaacct
      601 gcgagcagac aaagtgatgg tagcccgcga tggtaaacgc atgaccgata cagattctct
      661 ggcttactac ctggacaagg gcagaaccat ctggatgaac gacagctact ggctgttcat
      721 gccgttcaag ctgctggaca aaggagtcat cctccactac atgggcaaag gagaaacagt
      781 ggatggaaaa gagaccgaag tactggagct gaccttccgg caggtagggg ctacacctct
      841 caacaaatac cacattcata ttgaccccgg cacctatgac gtgctgcagt gggattatta
      901 tatcaggaca gacgaagagc agcccaccat catcaccccc tggacagact acaaatggta
      961 cggcaagatc cgcctgtctt ccggcagggg atcgttcggt aagatcacca atatcaaact
     1021 gcctgattcg cttcctgctt ttacttttcg ggatccggta aaacctcttc agggtttcta
     1081 gagttgcagg ccctcaaagc cacaaatggc tatcatttgt caacaactga attcaccttc
     1141 gcctaacgct atctttattt ttctcacatg cacttcagga tgtaatgaca ttttcataat
     1201 accatatact aattgagtta aggttcaatg gagagcttcg ccccaaactc aatccaagat
     1261 gaaacgaacc aacctatggc tcagaatggc aggtgcagcc cttgtgctgg ccatgaccgc
     1321 acagagtctg aaagctcaga atgcatttat tgaccttcag ccggcaggca ggtatgaaac
     1381 aatgaccttt ggagaaagcg caaccgaaat tgccgcttat gatccgcaaa gtcagcgatt
     1441 attttcaacc aatgccttta ccggacaagt agatgtgctg gatatcagtg acccggaagc
     1501 accggccctc ttgtttagta ttgacctgtc agcttacggt tcaggagcta acagcgtagc
     1561 atttcgaagt ggcttcctgg cttgtgctgt ggaggcagat gtcaaaacag acaacggcag
     1621 cgtggtattt tttgatgccg atggcaacta tgtgaacagt ctggaagttg gtgctctgcc
     1681 tgatatgatc accattactc cgaacggcga ttttgccctg accgccaatg aaggggaacc
     1741 aagcgatgat tacctcacag acccattggg taccatcagt attattgacc tgcgtgccgg
     1801 tattcctgca ctgacagcag atgacgtgac aacacttgat ttttctgcat tcaatgatgc
     1861 tgtcctggat ccctccatca aaataaatgg tcccggagct tctgtggcca tggatctgga
     1921 accggaatat attgctgtgt catccaactc aaagtttgcc tatgtaacta ttcaggaagc
     1981 gaatgcagtt gccattgtcc agatagaatc acccgcaatt gtcgggctgg ttggtcttgg
     2041 atttaaagac catatgctgc ctggaaacgg gctggatgca tcagataagg cagacaatat
     2101 cgacattgtt aactggccgg ttaaagggct ctttatgcct gatgcaatag gctttctgag
     2161 agccggtagc ggtgcctatc tgatcacagc caatgaaggt gatagccgtg attatgatgc
     2221 attcagtgaa gaagatcgta taaaagatgt tatactggat actgttgcct ttccgaatta
     2281 tgccggcctt cagaacgatg acagtatcgg gaggctgaag atcatcaata cgatgggtga
     2341 tattgatggc gatggcgatt atgatgaatt gtacacttat ggtgcccgca gtttttctgt
     2401 gtgggatctg gcaggaaacc tggtctatga ttcaggtgat gaactggagc aactgaccgc
     2461 tgcctggtat cctgataact tcaatgcttc caatgacaac caggacctga aaaaccgaag
     2521 cgatgataaa ggccctgagc ctgaggtcct cgccatcggg agaatattca agaacccata
     2581 cctgtttctc ggcctggaaa gaatgggcga gatcatggtg tacgatatga gcgatcctgc
     2641 ttcaccgtct ttcgtaaccc acaccaacag ccgcgatttc agtgttgatc ctgaagatga
     2701 tgcatccggc gattatggcc cggaaggcct cctgtttatt ccagccacac agagtccgaa
     2761 cggaagaaac ctgctggtta ccagtaatga ggtgagtggc agcattgcca tctattacac
     2821 tgactatgcc tgtggagcca gtaaagtttc cgtttgctat gacggagtaa gctactgtct
     2881 ccccattacg gtagcagaag ctttggttac acttggcgga actccgggtc cctgtgatgg
     2941 tatgcgcagc atgctgggtg aagaagatct cgcactgggc atcaacgctt acccgaatcc
     3001 tgcaagcgat catgtgaata tagtcctgaa tgatctgccg gccggaaact ggaacctgcg
     3061 tctgtttgac atcaccggtc gtatggtgtt taccggaaac gtagagaacg acggcagcaa
     3121 ctgggtaact tatccggttg atgtacagca aatgcctgca ggtacctata cctgccaggt
     3181 gaccagcaac aacggagtgg ctttcagcac ccgcattgcg gtggtacaat aacaagacct
     3241 gatttctata tatgtgtgtt ccggcggacc gaatggttcg ccggattttt tatgccagca
     3301 caaagtccag ctgacgggca tccagatcgg ccttctgtac tttgatgcga acagtttcac
     3361 ccatacggaa ggtgcggcct ccggtcatac ttctcaattc gcgtttagcc tcatcataga
     3421 tcagctcatc tctgacgctg tctgtccgca caagtccttc acatttgttc tgaaccagtt
     3481 ccacgaagat gccgtaatgc tgtatgccac tgataacccc atcgaactct tcacccacat
     3541 gtcccataag aaattccacc tgcttgtact tgacactttc ccgttcggct tccatggctt
     3601 tacgttccat ggccgatgaa tgtctgcatt tctcttccag ttcttccttg cttgcccagc
     3661 ggggcatgtt gtgcaactgg tggaaaagac tgcggtgcac catcaggtct gggtatctgc
     3721 ggataggaga ggtgaaatgg gtatagtgcg ggaaagcaag tccgtaatga ccgatattgt
     3781 tggtggtata aacagccttg gccatacaac gaatggccat ggtctcgagt acattctgtt
     3841 ccggcttgcc ctggatctcc ttcatcagtt cattcatcct ggcacttacc tgattcggtt
     3901 gatcaaaacg aaccttatag ccgaatcttc tggccatcat gctgaactcc tccagcctgt
     3961 ccatatccgg aaaatcgtgc accctgaaaa caaaaggtac aggcggctgg atcttagaca
     4021 tgaactcagc tactttcctg ttggccagca gcatgagatc ttcaatgagc atgtgagcag
     4081 cctttctttc cttgacataa agacccaccg gcttcttgtt ttcgtccagg atgaatttca
     4141 cttcctgggt ctcaaaagca acagctccct tcctgaatct ctcgttgcgc aaggcaagag
     4201 cgatcttgtt cagggtatgt atttctgcct tgtattcatc cagacgatct tcgatgacct
     4261 cctgcgcttc ttcataggtg aatcttctgt cgctgtgaat gacagatttt ccaaaccatt
     4321 ccttatgcac ctttccttct tcatccattt caaaaatggc actgaatacc agcctgtcct
     4381 ggtggggaac cagtgaacag atattattgg aaagtttttc cggaagcatg gggatgacac
     4441 gatccaccag ataaacggaa gtagcccggc tgtaggcttc cttgtcgatg gctgttccgg
     4501 gcgtgacata gtgcgacaca tcggcaatgt gcacacctac ttcccatcgc ccgttgtcca
     4561 gttgtttcag gctgatggcg tcatcaaaat ctttcgcatc gtgggggtca atagtgatgg
     4621 tccaggtact gcgcatatcc ctgcgcagcc tgatctcctc ttccggaatg tcattgctga
     4681 tcttctccgc ttcctggtcc acctctttcg gaaagacata ctgtatgcct gtctccacca
     4741 tgatggcctg catgtcactc tcgtgatcgc cggcgggttg caggcgggaa acgatctgac
     4801 cgatgggact cttcatggca ggcggccagt ctttgagccg cacgatgacc cgttcgccat
     4861 cctgggcttt gctgcgtctt acctcttccg gtgcaatgaa gaaatcgtag ttgttaaagg
     4921 ctttgtccgt ttccacgaat gcaaagccat cgttcatgcg caaatgaccg atgaacgtat
     4981 cacgggcccg tttgatgacc tccacgatct cgccttccgg cttgcctttc tttccggcac
     5041 gaagtctgac cctgaccgta tcgccgttga aggcacgatt ggtattgcgg gcagccacga
     5101 acacatcgtt gagtctgcca tctatcagca cataggcatt gccggaacgg gtaatgtcca
     5161 ccacgccgat cacagaactg ccaccactgt ctttctgtaa gaggcggaat ttatcctgct
     5221 ggtattcttc tatctgctgt gcatacagaa gatcttccat cgcatcgaag atgcctgctc
     5281 cctcggcctg tttcacagac cacttgagaa tgttcttgag cgactgccct ttggcaggtc
     5341 gttcgcctat gaattcaagc aggtctttgg ccagattgcc actgcgccct ccggttggtt
     5401 tattgatctt tttctttttg gccatactat ttgcctccaa gcagactacc cagcaatccg
     5461 ccgccgcctt tttgtgagga agcacctttc ttcaccatgt ccatgatatc gtccataccg
     5521 aacccgtcgt cgtccttttc ctgtttcagg atcttgctga aatctatacc tgaagactta
     5581 tttcctgtca gcgatccgaa tatatcattg atgtcaactg atgcatcatc cgggtcattg
     5641 gtctttttaa tgaagcgtga caagatcatc ggtatgagct gtgaggcaat accatcggct
     5701 ttcaaaccat ctacctgaaa cttagacccc agctgtttac tgaacatgct gctgatctgt
     5761 tgtaccattg ggttgtcagc acctgcatcg ccctggcgga acagttgcat cacatcctgt
     5821 aaattgccat tggcaagttg gtcgcgaagc gtatcctgaa tgctgttggc agcctcctgt
     5881 atagcgtctt cattgaattt gttatcgatg gcatcattgc gaacgatctg ttcatccgcc
     5941 tgctgcctga ctatttccag taattgttca aacatgcaat aaaggtacgg cgaagcggat
     6001 acttaatgtt tagtaattgt tgatttctca taaaattttc atcgcctccc tgtgctgttc
     6061 tggtgcactt tttaatttta catagcatga aatccctcta tatcatcagg catgccaagt
     6121 ccagctggga atacagtgaa ctgcacgatc tggagagacc acttacagac aggggcaaac
     6181 gtgatgctgt catcatggga acattgttgc gcaagcagaa tgtgcatccc ggtattgtga
     6241 tcagcagccc cgcactccgt gccatttcta cctgtcggat catatgtgat gagatcgggt
     6301 atgacaagag ccgcattgag atcaatacgt cactttactt caaggatgtg gaagagatcc
     6361 tggcagtcat acacagcaga ggctccaggc atgatgatat gttcctgttc gggcacaacc
     6421 ccaatttctc caatctggcg cacacctttt cacctgaatt tgatgagatc attcccactt
     6481 gtggcatagt ttgtattcgt tttgaagcag aaagctgggg gcttgcagaa gccggttccg
     6541 gtaaactgga gtactttgat atgcccaaga actaccgttg agcagcacta tgacatgccg
     6601 gatgcagatc gttagcaatg tcttttcagg ctgttaggct catgctcaca gccaggcaga
     6661 gctggttcac agatccagcc aggtgggatg aatgcagcac ttgtggtggt ggagaaaaat
     6721 gacactgacc tgaatggttt tccgtacaac accttacctt taaaccaatg gcttcttaca
     6781 tagctgagat cagattgctg aatgcaggtt cagatgcatt catgcaatgc ataccccgcc
     6841 agcgcatgat ggtgaacgat atgatggctg aaggccttat aaccagttat gcagtcagcg
     6901 ctgacaggaa gaaaatgtgg tgtgttgtgg aggcagattc tgtagatgaa gccacacgga
     6961 tcattgatca gtttcctctc agccctttta tggaagctga ggtcaaagaa ttgctgtttc
     7021 accatatgca cggttcactc atgggtacca tctccctgaa ctgattcctt tattatcttc
     7081 gttcatatga gtggccgact attgaccata ctgctgattc tggccggtag cagtttcctg
     7141 cttacagaca tgcatcattc cggcatgacg caggaagata cacctgaaaa tgaacttgca
     7201 gaagcctacg atgctttcgc tatcgacctg ctgaaaaagc aggaccccga aaccggtcgc
     7261 ataccggatg gtgcacttat gtcggcctac agaaccctga aagccagagg ttactaccgg
     7321 catgaacagg gcaataccag atctgaagac ggatggcagc aggtgaatga ttactttcct
     7381 tctctggcca ttacgaagat cacctacgat ccgaataata ccaatgtatt ttatttctgt
     7441 accggcgaag gatggttcaa cgcagatgcc gttcgcgggg ccgggatatt caaatcgacc
     7501 gatggcggag agacctggtt tcaattgcca tccacagatt ctgccatctt tcagtactgt
     7561 caggatattg acgtgcatcc cattaccggc gatatctatg tagccacccg ctcaggtgga
     7621 ttgcaacgtt ccaccaacgg cggagagtcc tggcagaaag tgctgggaag tggtgcagga
     7681 tccggacgaa acagcatctg tgatgtagag atcaccagcg atggcggggt gtttgtcgga
     7741 attggcatct tcgagaccga cggcatctat tattctgcca acggagatag tgccaccttt
     7801 tccaaacaaa ctgtcgggct tccttctacc ggatactatc gcattgaact ggctgtgtcc
     7861 aagtcagatc cggatatcgc ttatgccatt ccctgttcca ccagttttcg tatccagggc
     7921 atctaccgca ccgatgatcg tggtgccacc tggcaggcta ccaatctgcc cggcgatgac
     7981 aatgaactgg cagcacgtca ggcctggtac gatctggtca ttgaggtatc gccggaagat
     8041 cctaatctta tcattgccgg cggactgaac atctggcgaa gtactgacgg agggggtacc
     8101 tgggatcagc ttacgcgggg aagtctggac tcttccctgc tccgatacat gcacgtggat
     8161 cagcatgcgg ctgttttcag aaatcaggag gaagtgtatt ttggcaatga cgggggtatc
     8221 tataaaacac tcaatttttc tgccgataac cccaccatct acagcaggaa cgatggctat
     8281 aatgtgaccc agtattatgc ccatgctatg tccggccagg caggcagcga tgtgcttatt
     8341 ggtggtacac aggataatgg ttcgcacctg gcatataacc cgggtttatc ttcattcaaa
     8401 cccgtatcag gtgccgatgg agctttctgt gccatcaaca gtatcaatga agatattttt
     8461 tatacaacta cccagtatag aagactgttc agatttgata acggtggttt tgaaatgccg
     8521 gatacactga ccaatccgaa tgtaacagat gataacctcc tgttcattaa tcctattgag
     8581 atcgatccgg tcgatcctga aaatctgtat atggccagca atgccggact ctggcgtttg
     8641 atcaatgcgt ccaccgcaga tacatccggt tgggagaaag cctgtaccta tggaggaacg
     8701 atctctgcaa ttgctgtttc aacaaatccg cctaacctcg ttttcattgg cagaaatgta
     8761 agcaatgcag aacccgctgt gctgtatgac gccagttttg tggacggtgg ttcatcgcct
     8821 ttcgggctcg atccatccaa tatgatccct gacgcaaccg gttttggtag tacccgctac
     8881 tgctccagta ttgcagt
//
LOCUS       DAOMAX010000096         8850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1124_length_8850_cov_4.050142, whole genome shotgun sequence.
ACCESSION   DAOMAX010000096 DAOMAX010000000
VERSION     DAOMAX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_8850_cov_4.050142"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /gene="lon"
                     /locus_tag="PK742_06525"
     CDS             complement(<1..1890)
                     /gene="lon"
                     /locus_tag="PK742_06525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQU76280.1"
                     /translation="MRDYGFKEGIMTSVVDEDVEFLPMMSFDEDEEGLKAEVPDVLPI
                     LPLRNTVLFPGVVLPITVGRDKTIKAVADTFRGKKLIGVLAQRDADNEDPELEHLYPV
                     GTVARIVKQLRMPDGSTTIIIQGKGRFTAGEVVTTDPYLQTKATLLKESELPDETSFE
                     AIISSIRDLANDIIQQSPNIPSEAAIVLKNIDSPAFLIHFVASNLQIGVVEKQQLLEA
                     PGLQQRAEGVLKFLQSELKMLELKNQIQDRVKMDIDKQQREYFLSQQLKQIQEELGGS
                     PGEAEISRFMDRANRKKWNAKVREAFDKEIEKLRRIHPNAAEYSVITNYIDLLLELPW
                     EEFTTDNFNIKHAREILDEDHYGLDKIKDRLLEYLAVLKLKGDMKSPILCLVGPPGVG
                     KTSLGQSVARALGRKYVRMSLGGLHDESEIRGHRKTYIGAMPGRILQSLKRAKSSNPV
                     FILDEVDKVGSDFRGDPSSALLEVLDPEQNTTFYDNFLELEYDLSKVLFIATANNLNT
                     IQPALRDRMEIIYLSGYSAEEKIEIAKRHLVPRQRTEHGLKSREVILKDEVLRMLIEH
                     YTRESGVRELERVIASVMRRVARYVAEGEMYNKTIRKEELKDILGAPRFMKEDAAQLD
                     SPGVAI"
     gene            2036..4177
                     /locus_tag="PK742_06530"
     CDS             2036..4177
                     /locus_tag="PK742_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQU76281.1"
                     /translation="MRLLVWILLFHITASQAQQSFDGIWSSGNIPQDFIKTSGQKFQA
                     DIATIDQNLKRSEQRTRESFYLQSNYVFDQLLKGGSITFGDPVTKYLEGVLDKVLAAD
                     PKLRKELRIYTFKSTIPNAFTNGSGIILVTTGLLAQLENEAELAFILAHEAVHYREQH
                     AINNYVENTRLLQGRGLYRNLSAEEVELTQFQYSRDLETESDLEGLEYYLKTDYQPDA
                     AIAAMDVLLYSYLPFEEKPYDLHTWVNDYYGAMEKYTIPESAVNEIEAEEDYDDSHST
                     HPNIKSRKAELSEKLATIRSSGQALFLVADENRFLSLRDTCRYQNIMLNLRDLNYEAA
                     LYDVYMLQEKYPEDPQLEKWRFRCAYSLAMYETYRRTPSGQTSYKKVEGNSQPVYYFF
                     QKLDKEDLICMAIRAGWKYVQANPDDSESRRMLKGLAYEFLYSTDLSLDAFMNEEAAV
                     RLLSDTSATDIDDAGTKVTNIKKKKNQQEKEAYLNLFAEFIDDPSFRSFFDQQEISVN
                     SDTDNEPESLHASRVLLIEPIYLRFDTRSDQPLKFEASAEAQANLETLSKSICSKLGI
                     KLEYLDKHVTKGETADKLNELARLNDWFNEELDHLDADVPVINSSDVPFRQVVTAHNC
                     SHVMWAGMASVRERSLNIFSAILGGFYFPPYLPFLLVESFTPAYTTQAFGLVADAETG
                     IIEAGYDHSISMKDTPAVQKSLLYNMIKILK"
     gene            4174..4965
                     /locus_tag="PK742_06535"
     CDS             4174..4965
                     /locus_tag="PK742_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76282.1"
                     /translation="MKKYLYFIFAFILFANNTHAQVSGYLGKRAWVNLSVPIGLQLPD
                     LSDLYEEVPAARIYLRPGLSASYLVSENVDLGISYQRSSVKGYNIRISDQRDFNSAYY
                     YGNMAIKSNMFSFFLEFHNKGSFGSLPPLGKFYRFEILLARGKPVYSEASTPFSYSPL
                     FSLSDFPGAEDFSYSLPELLYTLGTRTIFFEGLVFEYGAQLGIPLSGAGWYSMINYAN
                     GERNLYTDYANDPEDAQEQFANLMRGYVVRSMIMNFHLSIGYLAF"
     gene            complement(4966..6786)
                     /locus_tag="PK742_06540"
     CDS             complement(4966..6786)
                     /locus_tag="PK742_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQU76283.1"
                     /translation="MRLYLLIIATMAVFYTVHAQPAIGLETYASGFTYPLHVTNAGDE
                     RLFVVQRQGKIRIIDGSGTVLATPFLDITSQIESGYEERGLLGLAFHPDYESNGYFYV
                     SYTDLSGNSVISRFSVSADPDLADEDSEYIIYSATQPYSNHNGGCIHFGPDGYLYIGL
                     GDGGSAGDPGNRAQTMTNKLGKIHRIDVDGGDPYAVPADNPFAGAVDTLETIWASGLR
                     NPWRFSFDRETGDMWIGDVGQNLWEEIDFQPAGEGGNNYGWKCYEANHSFALAGCDDE
                     SAYVFPVAEYGHSFGTGGYSITGGYRYRGADFPAMYGYYLYTDFVSGNWWWIREGVAG
                     EWEIAFLDYIEDDIAGFGEDMNGELYCADLYGGTIYRIVDLCGDFLATAVADSFYCDA
                     APGAIDLTITGGAGPYTILWNTGSTDEDLSGLEAGIYSVGVFDDEGCVRELNVEVPTG
                     TVPVPAISFDGTTLTADGGIAWQWYLDGEAIPAATLSTFTPVDNGIYSVEVTWDNGCA
                     AVSDDLDVMLNGLTDLPAGWNLYPNPVSDQLILQVASDMQAVQMSLRDLQGRMVWETS
                     MNLLAAGTDLVIDCTGFAAGTYQLVISGNGETRMLQVLKQ"
     gene            6917..7798
                     /locus_tag="PK742_06545"
     CDS             6917..7798
                     /locus_tag="PK742_06545"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HQU76284.1"
                     /translation="MKNISIAAILLLGTFAFSSCAKERIEGCTNPYALNYNPKASDEN
                     GSCWIPVSTHKILFADFNATWCGPCGDWGAPAFSGAVNLTEGFAEPMSIHTSDEMSNP
                     ISEEFMMFYGITGIPTLKVWNSENSFSDSTGIAGAVVAELAEEPLAEASALVVMTDMG
                     NSYELGVSFTPFETIVGDYFVAVYIMQDGLNFPQNGAGEDGAYDPDFIHNHVLLGSAS
                     NTMFGEQFVYGNGFAGQLVTKVYSVPKEASWVEENMYALGVVWKYSKDKTTGEFIYEV
                     VNVTSSRDIVNTGASTL"
     gene            complement(7846..8559)
                     /locus_tag="PK742_06550"
     CDS             complement(7846..8559)
                     /locus_tag="PK742_06550"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQU76285.1"
                     /translation="MTKLSVNINKVATIRNARGGRVPDLVKFAQDCERFGAEGITVHP
                     RPDERHITLQDVYDLAPVVTTEFNIEGYPDERYMQIIRDIRPAQATLVPDPPDVLTSN
                     NGWDAIARQDELKLLVDELHGYGVRVSIFLNPDLAQVMAAKATGTDRIELYTGPYAEE
                     FPSGKEEAVVPYLKAAAAARSIGLGLNAGHDLSLENLAYFRQCIPWLDEVSIGHAIIS
                     DALYYGIENTIQLYLRRLA"
BASE COUNT         2267 a         2181 c         2097 g         2305 t
ORIGIN      
        1 gatggctacc cccggagagt ccagttgtgc cgcatcttct ttcatgaacc tgggggcacc
       61 aagaatatct ttcagttctt cctttctgat ggtcttattg tacatttcac cttctgccac
      121 atatctggcc acccgacgca taacagaggc aatgacacgc tccagctcac gcactccgct
      181 ttctctggtg taatgttcga tcagcattct aagcacctca tctttcagga tcacttcacg
      241 gcttttcagg ccgtgctccg tgcgctgtct cggaaccaaa tggcgtttgg cgatctcgat
      301 cttctcttca gcagaatatc cgctcaggta aatgatctcc atacggtcac gcagtgcagg
      361 ttgtatggta ttcagattgt tggccgtagc aatgaacaat actttgctca gatcatactc
      421 cagttcgaga aaattatcgt aaaaagtggt gttctgctcc ggatccagca cttccagcaa
      481 ggcgctggac ggatcaccgc gaaagtctga tcctacctta tccacttcat ccagaataaa
      541 gaccggattg gatgattttg cacgcttcaa ggactgcagg atcctgcccg gcatggcacc
      601 aatataggtc ttccggtggc cgcggatctc cgattcatca tgcagaccgc ccaggctcat
      661 gcgcacatac tttctgccca atgccctggc aacactctga ccaagtgatg tctttccaac
      721 acccggagga cccaccagac acaggatagg agatttcata tcgcctttca gcttcagcac
      781 tgccagatat tccagcaaac ggtctttgat cttatcgaga ccgtagtggt cttcatccag
      841 gatctcacgg gcgtgtttaa tattaaaatt atctgtggtg aattcttccc acggtagctc
      901 cagcagcaga tctatgtagt tggttataac actgtattca gcagcattgg gatggatgcg
      961 gcgcagtttt tctatttcct tatcaaaggc ttcacgcact tttgcattcc atttttttct
     1021 gttcgccctg tccataaaac ggctgatctc agcctctccc ggagaaccgc ccagttcttc
     1081 ctggatctgt ttgagctgct ggctgagaaa atactcccgc tgctgcttgt ctatgtccat
     1141 ttttacacgg tcctggatct ggtttttcag ttccagcatc ttgagttcgc tttgcagaaa
     1201 cttcaatacg ccttctgctc tctgctgtaa accgggagct tccagcaact gctgtttttc
     1261 taccacacct atctgcaggt tggaagccac aaaatggatc aggaatgccg gcgagtcaat
     1321 gttcttcaga acaatggcgg cttcactggg aatattgggg ctttgctgaa tgatgtcatt
     1381 ggccaggtcg cggatggaag agataatggc ctcaaatgat gtttcatcgg gcagttcaga
     1441 ctctttcagc agggtagctt tggtttggag gtagggatct gtggtgacca cctcgccggc
     1501 ggtgaaccgg ccttttccct gaatgatgat ggtggtggaa ccatcaggca tgcgcaattg
     1561 cttcacgatc ctggcgacgg ttcctaccgg atagagatgt tccagctccg gatcttcgtt
     1621 gtcggcatcc cgctgggcca gcacgccgat cagttttttt ccacggaagg tatcagccac
     1681 agccttgatg gtcttgtctc tgccaaccgt aataggcagc accacacccg gaaaaagcac
     1741 ggtgttgcgc aggggcagaa taggaagcac atctggtact tcggccttca gaccttcttc
     1801 atcttcgtca aaagacatca taggcaggaa ctccacgtcc tcgtccacaa cagatgtcat
     1861 gatgccctcc ttaaaaccgt aatctctcat cccctttcag ttggtacaat attatgatga
     1921 atcaggtcaa tgattatgcc accggcctca gtctgaccaa acggcagata attgctgtca
     1981 aaatttccac ttacatatcg ccagatttta tttacttgcc tgcaaatcca tgaacatgcg
     2041 cttacttgtt tggatacttt tgttccacat taccgcttca caggcacagc agtcctttga
     2101 cggcatttgg tcatcaggaa acattccaca ggacttcatc aagacctccg gacagaaatt
     2161 tcaggcagat attgccacta ttgatcagaa ccttaagaga tcagaacaac gcacccggga
     2221 gtcattctac ctgcaatcca actacgtttt tgaccagttg ctcaaaggtg gctccatcac
     2281 ttttggcgat cctgttacca aatatctgga aggagtgctg gataaggtgc tcgcagcaga
     2341 cccgaaactg cgcaaagaac ttcgcatata cacgttcaaa tctaccatac caaatgcctt
     2401 taccaacggc agcggcatca ttctggtaac caccggcctg ctggctcaac tggagaacga
     2461 ggcagaactg gctttcattc tggcacatga ggctgtgcac taccgtgaac agcacgctat
     2521 taataattat gtggagaata ccaggttgct gcaaggcaga ggattgtaca ggaacctgag
     2581 tgcagaagag gttgaactta ctcagtttca gtacagccgg gacctggaaa ccgaatcaga
     2641 cctggaaggg ctcgaatatt acctgaaaac agactatcag cctgatgcag ctatagcagc
     2701 catggatgtg ttgctgtaca gttacctgcc gtttgaagaa aaaccgtatg acctgcatac
     2761 ctgggtcaat gactactatg gtgctatgga aaaatatacc attcctgaaa gtgccgtcaa
     2821 tgagatagaa gcagaagaag actatgatga cagccacagt acccacccga atattaaaag
     2881 cagaaaagct gaattgtctg aaaaactggc aacgatcaga tcatccggtc aggctttgtt
     2941 cctggtggca gacgaaaacc gtttcctgtc acttcgtgat acctgccgct accagaacat
     3001 tatgctcaac ctgcgagacc tgaattacga agctgcgcta tatgacgtct atatgttgca
     3061 ggaaaagtat ccggaagacc cgcaactgga aaaatggcgc tttcgctgtg cgtattctct
     3121 ggccatgtat gaaacctacc gccgcactcc atccggccag acctcctata aaaaagtaga
     3181 gggaaacagc cagccggttt attatttctt tcagaaactg gataaagaag acctgatatg
     3241 tatggccatc cgggccggat ggaagtatgt acaggccaat cccgacgatt cagaatcccg
     3301 gaggatgttg aaagggcttg catatgaatt cttgtattcc actgaccttt cactggatgc
     3361 atttatgaat gaggaagcag ctgttcgcct tctttcagac acttcggcca ctgatattga
     3421 tgatgccgga acaaaagtga ccaacatcaa aaagaaaaaa aatcagcagg aaaaagaggc
     3481 gtatttgaac ctgtttgctg agtttattga tgatccatca ttcaggtctt tttttgacca
     3541 gcaggagatc agtgtcaact cagatactga caatgagcct gaatcccttc atgcatccag
     3601 agtgttgctc atcgaaccga tctacctgcg ttttgatacc cggtcagatc agcctttaaa
     3661 atttgaggcc tcagctgaag cccaggccaa cctggaaaca ctgtcaaaaa gcatctgcag
     3721 caaactgggt atcaaactgg aatacctgga taagcatgtt accaagggag aaactgcaga
     3781 caagctgaat gaactggcca gattgaatga ctggttcaat gaagagctgg atcatctcga
     3841 tgctgatgta ccggtcatca acagcagcga tgtgcctttc aggcaggtgg ttacagcaca
     3901 caattgcagc cacgtaatgt gggccggcat ggcatcagtg agagaacgaa gcctgaatat
     3961 cttcagtgcc attttgggtg gtttttattt tccgccttac ctgcccttcc tgctggttga
     4021 atctttcact ccagcttata ccacccaggc attcggactg gtagcagatg cagaaacagg
     4081 tatcatagaa gccggttatg accatagcat cagcatgaaa gatacgccgg cggtacaaaa
     4141 gtctttattg tacaacatga tcaagatttt aaaatgaaaa aatatttata ttttattttt
     4201 gcttttattt tatttgcaaa caacacccat gcacaggttt cgggctatct gggaaaaaga
     4261 gcctgggtca acctgtctgt acccattggc ctgcaactgc ctgatctatc tgatctctat
     4321 gaagaggtgc ctgctgccag gatctacctc aggcccggat tgagtgcttc ctatctggtc
     4381 tcagaaaatg tggacctggg catttcctac cagcggtcat ctgttaaagg gtataacatc
     4441 aggatatcag atcagagaga tttcaatagt gcctattact atggcaacat ggccattaag
     4501 tccaatatgt tttcattctt tctggaattc cataataaag gatcttttgg ctctctgcct
     4561 ccgctcggta aattctatcg ttttgagatc cttcttgcca ggggtaaacc tgtttattct
     4621 gaggcctcca cacccttcag ctattcaccg ttattcagtt tgtcagactt cccgggtgca
     4681 gaagatttta gttattcgct gcctgaacta ctctataccc tgggcacccg aaccatattc
     4741 tttgaaggcc tggtatttga gtatggtgct cagcttggta ttccgttatc tggtgcgggc
     4801 tggtacagta tgatcaacta tgccaatgga gagcgtaatc tgtacaccga ttatgccaat
     4861 gaccccgaag atgcccagga acagtttgca aacctgatgc gtggatacgt ggtacgctcc
     4921 atgatcatga acttccacct gagtatcggg taccttgctt tctgatcatt gcttcagtac
     4981 ctgtagcatt ctggtctctc cgttaccgga aatgaccagc tgataggtac cggcagcgaa
     5041 ccctgtacaa tcgatcacca gatctgttcc ggcggccagc aggttcatgg aagtttccca
     5101 gaccattcgc ccctgtagat cacgtaaact catttgtact gcctgcatat ctgaagctac
     5161 ctgcaggatc agctggtctg atacgggatt aggatacaga ttccatccgg caggcagatc
     5221 ggtcagtccg ttgagcatga catccagatc atctgaaact gctgcacaac cgttatccca
     5281 ggtcacctct acgctgtaga taccattatc gaccggagtg aatgtactca gagtagcagc
     5341 cggtatcgct tctccatcca ggtaccattg ccaggctatt ccaccatcgg cagtaagagt
     5401 agttccgtca aaactgatag ccggtaccgg aacggttcct gtgggtactt ccacattcag
     5461 ttcacgcaca catccctcat catcaaacac acctaccgaa tagatgccgg cttccagtcc
     5521 ggacagatct tcgtcggtag aaccggtgtt ccaaaggatg gtatatggcc ctgcgcctcc
     5581 tgtaatggtc aggtcaatgg caccgggagc agcatcgcaa taaaagctgt cggccacagc
     5641 agttgccaga aaatcgccgc aaagatccac tatcctgtag atggtgcctc cgtacagatc
     5701 tgcacaatac agttcgccgt tcatgtcttc gccaaagccg gcaatgtcat cttctatata
     5761 atcaaggaat gctatttccc attcgccggc cacaccttcc ctgatccacc accagtttcc
     5821 tgaaacaaag tcggtgtaca gataatagcc atacatggca ggaaaatcag cacctctgta
     5881 acggtatccg ccggtaatac tgtagccacc ggtaccgaaa ctgtgcccgt attcggcaac
     5941 aggaaaaaca taggcagatt catcatcgca tccggccagt gcaaaactgt gattggcctc
     6001 atagcatttc cagccgtagt tgtttccacc ttcaccggca ggttgaaagt caatttcttc
     6061 ccaaaggttc tgtcctacat cgccgatcca catatcgcct gtttcccgat cgaaactgaa
     6121 tctccaggga tttcttaatc cggaggccca aatggtctcc agcgtatcaa cggctccggc
     6181 aaacggatta tcggccggaa cagcataggg atcgccgcca tctacatcaa tgcggtgtat
     6241 cttgcccagc ttattggtca tggtttgtgc ccggttaccg gggtcgccgg cagaacctcc
     6301 atcacccagg ccaatataga ggtagccgtc ggggccaaaa tggatgcatc ctccgttatg
     6361 attggagtat ggctgcgtgg ctgaatagat gatatactca gaatcttcgt cagccagatc
     6421 aggatcggcg cttacgctga atctgctgat gacagaatta ccggaaaggt ctgtatagct
     6481 tacatagaaa tatccgttgc tctcgtagtc ggggtggaat gccaggccca gcagcccacg
     6541 ttcttcataa cctgattcga tctggctggt gatgtccaga aaaggagtgg ccagaaccgt
     6601 tccgctgcca tcaatgatgc ggatcttacc ctgacgctgc accacaaaaa gacgctcatc
     6661 gccggcattc gtgacatgca aaggataggt gaatcctgat gcataggttt ccagtccgat
     6721 ggcaggttgt gcatgaactg tgtaaaaaac ggccatagtg gcgataatga gcagatatag
     6781 gcgcattttg aaacttttgt gctaagatac gcaactgtgt aactgtctta ttgtaagtac
     6841 tgtgtcagat cttcccctgc cctgtttgga attgtttcaa attgcctgta tttttaaacc
     6901 tcaaatcgat caaccgatga aaaatatttc tattgcagca atactcctcc tgggtacctt
     6961 tgcattctct tcgtgtgcga aggaaaggat cgagggctgt accaatcctt atgcactgaa
     7021 ttacaaccct aaagccagtg atgagaacgg cagctgctgg atcccggtgt ccacgcacaa
     7081 gatcctgttt gccgatttta acgctacctg gtgcggtcct tgcggcgact ggggagcacc
     7141 tgccttcagt ggtgccgtca atcttaccga aggttttgct gaacccatgt ccatccacac
     7201 ttcagatgag atgtccaatc ccatctctga ggagttcatg atgttctatg gcattaccgg
     7261 tattcctact cttaaggtat ggaattcaga aaattcattt tctgacagta ccggaattgc
     7321 aggtgcagtg gttgccgaac tggcagaaga acctctggca gaagcatcag cactggtggt
     7381 catgacagat atgggaaatt cctatgaact gggtgtttct ttcactccgt ttgaaactat
     7441 tgtaggcgac tatttcgtag cagtctatat catgcaggat ggtctcaatt tcccacaaaa
     7501 cggcgcaggt gaagatggcg cttatgatcc tgatttcatc cataatcacg ttttactggg
     7561 ttctgcttcc aacaccatgt tcggagagca gttcgtatat ggaaacgggt ttgccggtca
     7621 gctggtcacc aaagtttatt cagttcctaa ggaagcttcc tgggttgaag aaaacatgta
     7681 cgcactgggt gtggtctgga agtattccaa agacaaaaca accggtgagt tcatttatga
     7741 agttgtgaac gttacttcca gccgcgacat cgtcaacacc ggcgcttcta ctttataatt
     7801 ctgtttcaga aatcatataa gccgttcctg ttggagcggc tttttttatg ccagccggcg
     7861 aaggtatagc tgaatggtat tctcaatacc ataatacaag gcatcactga tgatggcgtg
     7921 gccgatggat acttcatcca gccagggaat acattgtctg aaataagcca gattctccag
     7981 actgaggtca tggcctgcat tcagtcccag gccgatgctt ctggcggccg ctgctgcttt
     8041 cagataaggt acaacggctt cttcctttcc tgatggaaac tcttccgcgt agggacctgt
     8101 atataactcg atccgatccg taccggttgc cttggcagcc atgacctgtg ccagatccgg
     8161 attcagaaat atggataccc gcaccccgta tccgtgcagt tcatctacca gtagtttcaa
     8221 ttcatcctgc cgtgcgatgg catcccagcc attgttgctg gtcagtacat ccggcggatc
     8281 aggtaccagg gtagcctgag cgggacgaat gtcgcggatg atctgcatat atctttcatc
     8341 cgggtagcct tctatattga attcggtggt caccaccgga gccaggtcat agacatcctg
     8401 cagggtgata tgacgctcat ccggacgcgg atggacagtg atgccttcag cgccgaaacg
     8461 ctcacagtcc tgtgcaaact tcaccagatc aggcactctg ccaccacggg cgttgcggat
     8521 ggtggctacc ttgttgatgt tgacggagag tttcgtcatg ctgcaaaact aagtagctaa
     8581 tgagaaaatg gaggaatggg gaatgagaga atgtgaaaat gagggaatgg ggaaatgaga
     8641 gaatgtgaaa atgaggaaat gggggaatgg ggaaatgagc tgatgagggt attagccgat
     8701 tagccgatgg aagggaatgg agggaatggg gaaatgagag aatgtgaaaa tgaggaaatg
     8761 gggaaatggg aaaatgagct gatgagggtt ttagccgatt agccgatggt agggaatgga
     8821 gggaatgggg aaatgagaga atgtgaaaat
//
LOCUS       DAOMAX010000097         8783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1139_length_8783_cov_4.341086, whole genome shotgun sequence.
ACCESSION   DAOMAX010000097 DAOMAX010000000
VERSION     DAOMAX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1139_length_8783_cov_4.341086"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..569
                     /locus_tag="PK742_06555"
     CDS             63..569
                     /locus_tag="PK742_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76286.1"
                     /translation="MRWLLLFLICPLISEAQTNDGFFLFSVADTASHWLVTGQDHVQA
                     QVQVGETEDMFLPVVQVGSSEWMALRIPYEFLGQPMLIRISRPSGNMEEVMELQLATA
                     VPGSMEGCYRCMAGSIIFSKARVVIDMPKQPESWELLPRRNVTAANTSFDCYDLSILQ
                     PFCAVKRN"
     gene            584..1546
                     /locus_tag="PK742_06560"
     CDS             584..1546
                     /locus_tag="PK742_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HQU76287.1"
                     /translation="MQILLFVIGLGLVILGARLLVEGASSLAKKWRVSELVIGLTIVA
                     FGTSTPELVVNLYASFQGSADLAIGNVVGSNIFNILVILGISSLIYPLTVQTFTVLRE
                     IPFSLLGAIVLLIMANDFLLDGNAQDLISRADALVLLGFFSIFVYYSFIIARSVSETP
                     EVTIMERSTLRSILMVAGGLTGLTLGGKWMVDSAVQMARVMGMSESVIGLTILAIGTS
                     IPELATSVTAALRKKSDIAVGNVVGSNIFNIFFILGISGTARPLFVSTGTNRDLLVLL
                     LSTSMLFFLVYFHKRKQLQHWHGIIFILMYVFYLAFLLSGRVVF"
     gene            1592..2899
                     /locus_tag="PK742_06565"
     CDS             1592..2899
                     /locus_tag="PK742_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76288.1"
                     /translation="MRRFYSFLGLLAIAGMAMAQPVVNGDLSDADYVVIGNKLNSNAG
                     FGGSIDVSQILYYPDDAAGILYLGFEGRLNTGSNDGLGVLINVAGAGSPVGWGAGSNL
                     AFGGGGHFLDQTFYADFEVDYGFAFNPGFWVTDVYWDAVKYTGGGGAEYQGNCNQTGG
                     AAANSSAGGAVFSNGSVTFAFHNGGGAGQGLEMAIPYSELGATADMDIEVAAFIVSES
                     AFFSDVTVPGNVTTGNLGFGPNFGFIGGGPYHTGPDALSLFVCEAPAGLAVTGLTATT
                     ATLSWNAVTGATGYVLQLSNEVISSGKRVKLGAGITSVNLGPGQLVPGTDYSWGVRTR
                     CDGEMSSWSAMQNFSTPLRLAGGEDDLALVYPNPSNGQFTVSLPAQGNGSVVIKVIDL
                     QGRIVSMVNSGDSNVQIHVENPGVYQLWIQYDTVSFVQQVIIQ"
     gene            3087..4322
                     /locus_tag="PK742_06570"
     CDS             3087..4322
                     /locus_tag="PK742_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76289.1"
                     /translation="MRKTILALTACISMILPAHAQLSAGSLMFVAFNADGTDGVAFAA
                     LEDVPAGTIIYFTDNEWDGSSFNTGETYWQWSSGSTLTAGSVVIIEGIDQGTLNGSSP
                     NPTASVGSCSWSTTSSPDNNSGLSASGEAVYMYLAASYNMPTTFISAIATGNFNPSLG
                     ELTGTGLTEGVNAIQVLGNSDVIIFYTDVFGADCVETIATEGNWVEDNGSGDQSNDGN
                     YPDFPADVIDFLGCICSCETPVGLTASAITPYSATLSWTAVAGATGYVIQYENELTGP
                     NYRGKISDGTTSVNVGAGKIQPGNSYVWGVKARCADCYSDWSEPSYFTSPMRLAGGDD
                     AVALIFPNPSNGQMTIQLPESNAETMIMITDLSGKMVLSQSVGAGETAVQLNIAVPGM
                     YQVLISSNESSFIQKVIVQ"
     gene            complement(4386..6881)
                     /locus_tag="PK742_06575"
     CDS             complement(4386..6881)
                     /locus_tag="PK742_06575"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQU76290.1"
                     /translation="MKYHYLLSLAFFFQTELAYSQLNGEKRTLQAVRTEESIRIDGIL
                     DEAVWQQADAATDFTQFQFQWKVPSAYKSEVKIVYDNNALYVGAMLYDPSPDSIRKQL
                     SQRDELGATDFFGIALDTYKDGINGFEFIVSAGGVQLEAKTINGGNEDFNWDAVWQSA
                     AHINEKGWVVEMKIPYSAIRFPNQDAQEWGLQFFREVNRSRDKSTWSPIDPEIAGFTL
                     QQGTLLGVNGIEAPIRLSLTPYLSAYYDVFNDRPNDQVSHATSYNGGADLKYGLNDAF
                     TLDMTLIPDFGQVQSDNQVLNLTPFEVYFVERRQFFTEGVELFDKGNLFYSRRVGGTP
                     YAFWDVYGNLGDGETVINNPQQARLLNATKVSGRTQGGLGIGVFNAIEGETYATIEDA
                     EGASREELTGPLTNYNVVVFDQNLKNNSNAYLINTNVTRNGGFDDANVTGGGFALKNE
                     KQTYGAEGKFIASQQYRGGQNSADMGYAHSLYAGKIGGNFQATAGYWFEGPSYDPNDL
                     GFLQNNNTVNYGIQLQYNHFDPIWHINSLYTSGGVDYTRIVDPNEFFNFSGWLNVTAG
                     FQNFLFAGMFWATEPVVTYDWFEPRSPGRYYTFPVNNNIGGFVSTDYSKRIAFDLNAN
                     FRWFAEEVNRDIEGKRSRVNVYFSPRVRFSDKLTLILDQSVSVWPNDIGWTDPSIGWE
                     EIPENFVLLGRRDYSEYSSVINLAYTPTVKMGFTFRLRHYWSFATYQEFYELGDEDGL
                     LNPTTYNTSNALGGTDNDANFNAFNIDFVYTWFFAPGSEMNVVWKQAIYQFGDYVYPN
                     YGDDLEAVFNSPVGNNFSVKVIYYLDYLYLRKK"
     gene            complement(7008..>8783)
                     /locus_tag="PK742_06580"
     CDS             complement(7008..>8783)
                     /locus_tag="PK742_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQU76291.1"
                     /translation="LVARNGAGKSTLLKVIMGTEIADSGTVVINKEIRTGFLEQEPKL
                     IAGQSIMENVFSGNTPVLQAIGAYEQCLEAMEEDHSPAHAARLEEAHAQMDALEAWDY
                     EQKIRLILSKLDIHHLDQPVETLSGGQAKRVALAKMLIQEPDLLILDEPTNHLDVAMI
                     EWLEEYIGTRDMTMLVVTHDRYFLDSVCNQIIELEDGKLFNYSGNFSDFLEKKAHREA
                     VEAAELDKARNLYRRELEWVRRMPKARTTKSKSRLDAFDTLSEKASGKGPEEQLKLEV
                     KMNRIGGKILEMVKVRKAFGSLVITDGFTYTFKKGERVGIVGKNGVGKSTFLNMIMGM
                     EHADSGKIQAGETIVFGYFSQEPLRVADDKRVIDVVKDIAEFIPMADGSKLSASQLLT
                     RFLFAPETQYTFVSKLSGGEKRRLQLMTILIRNPNFLILDEPTNDLDILTLSILEQFL
                     LDYTGCLMIVSHDRYFMDKLVDHLFVFEGDGLISDFTGNYTEYRQAADKQNKEDKKKV
                     TAPKPKQAAEAVKKKRSFKENREMEMLEKDIAALEQEKQNIEQQLSSPTGDHQAIGVL
                     SERLMIVSNELDEKSMRWLELAELE"
BASE COUNT         2059 a         2141 c         2083 g         2500 t
ORIGIN      
        1 gattccggaa cgcaaccacc gtactgtagt ctgaattgat tactttagag caaaatccgc
       61 tgatgcgctg gttattgctt ttcctgatct gtccgttgat ttctgaggct caaacgaatg
      121 atggtttctt cctgttttca gtggcagata ccgccagtca ctggctggta accgggcagg
      181 atcatgtgca ggctcaggtg caggtgggag aaacagaaga catgtttctg ccggtggtgc
      241 aggtaggcag cagcgagtgg atggctttgc gcattccata tgaatttctc ggacagccca
      301 tgttgatccg catctccaga ccgtcgggaa atatggagga ggtcatggaa ctgcaactgg
      361 caacggcagt gcctggcagt atggaaggct gttaccgctg catggccggt agcatcattt
      421 tcagcaaggc cagggtggtc atagatatgc cgaagcaacc cgaaagctgg gaattgcttc
      481 cgcgacgtaa tgtaacggcc gccaatactt ctttcgattg ttatgacctg agcatcctgc
      541 aacctttttg cgctgtaaag cgtaattaga cagaacatat tccatgcaaa ttttgctgtt
      601 tgtcatcggt ctcgggctgg tgatcctggg tgcccgcctg cttgtagaag gggcttcctc
      661 ccttgccaag aaatggagag tgtcagaatt ggtcatcgga cttaccatcg tggcattcgg
      721 cacctctaca ccagaactgg ttgtaaacct gtatgcttct ttccagggca gtgctgatct
      781 ggccataggt aatgtggtgg gcagtaatat cttcaacatt ctggtaatac ttggcatatc
      841 gtcattgatc tatccgctca ccgttcaaac attcaccgta ttacgtgaaa taccattcag
      901 ccttttaggt gccatcgttt tgttgatcat ggctaatgat tttctgcttg atggaaatgc
      961 ccaggatctg atcagcaggg cagatgctct tgtcctcctg ggtttcttct ccatcttcgt
     1021 ctattactct ttcattattg ccagatcggt cagcgaaact cctgaagtta ccatcatgga
     1081 aagaagcact ctcagaagta ttttaatggt agccggaggt ctgacaggtc taaccttggg
     1141 ggggaagtgg atggttgaca gtgctgtgca aatggcccgg gtcatgggaa tgagtgaatc
     1201 tgtcattggc ctgaccatac tggcgatagg tacttctata cctgaactgg ctacttcagt
     1261 aacggccgct ctccggaaga aaagcgacat tgctgttggc aatgtggtgg gcagtaacat
     1321 cttcaatatt ttctttattc tgggaatcag cggcactgct cggccactat ttgtcagtac
     1381 cggcaccaac agagatctgc tggtcttgct attgtccacc agcatgcttt tctttctggt
     1441 ttattttcat aaaaggaaac aattacaaca ctggcacggt attattttca tccttatgta
     1501 tgtattttat ctggcttttc ttttatccgg acgggttgtt ttttgacata tatccgaaag
     1561 actgtagatt ggccatctaa acttgaaaac catgagaaga ttttactcct ttttagggct
     1621 actggccatt gccggtatgg ctatggcaca gccggtcgta aacggcgacc tctcagatgc
     1681 agattatgtg gtaatcggta ataaactcaa cagcaacgca ggttttggag gcagtatcga
     1741 tgtctctcag atcctgtact atcccgatga tgcagccggt atcctgtatc tcggatttga
     1801 gggcagactg aataccggtt ccaatgacgg attaggggtt ctgatcaatg tagcaggtgc
     1861 aggatctccc gtcggttggg gtgcaggcag caacctggct tttggcggag gggggcactt
     1921 cctggatcaa accttttatg ccgatttcga agtggattat ggatttgcat tcaatcccgg
     1981 attctgggtt acagatgtgt actgggatgc agtaaaatat acaggtggcg gcggagcaga
     2041 ataccagggt aattgcaacc agaccggtgg tgctgctgcc aatagttctg ctggtggtgc
     2101 agtgttttcc aatggcagtg tgacctttgc cttccataat ggcgggggcg ctggccaggg
     2161 tctggaaatg gccatacctt attcggaact gggagcaacc gctgatatgg atattgaagt
     2221 agcagctttc attgtaagtg aatcagcttt tttctcagat gtgaccgtgc ccggaaatgt
     2281 gactaccggc aaccttggtt tcggtccaaa tttcggattt atcggtggcg gtccatacca
     2341 taccggcccc gatgcactat ccctgtttgt gtgtgaagcg cctgcaggac tggcggttac
     2401 cggcctgact gctaccacag cgaccctcag ctggaacgca gtgacagggg ccaccggata
     2461 tgtgctacaa ctcagtaacg aagttatctc atcaggtaag cgggtgaaac ttggtgccgg
     2521 tattaccagt gtcaatttag gtcctgggca gcttgtaccc ggaactgatt attcatgggg
     2581 agttcgtact cgatgtgacg gagagatgtc ttcctggtct gccatgcaga atttcagcac
     2641 cccactccgg ctggctggtg gtgaagatga cctcgctctt gtctatccaa acccgtccaa
     2701 tggacaattc acggtcagtt tgccggcaca gggtaacggg agtgttgtta ttaaagtaat
     2761 tgaccttcag ggcagaatag tgagtatggt caattccggc gattccaatg tgcagattca
     2821 tgtagagaat cccggcgtgt atcagctctg gatacaatat gatacggtca gctttgtaca
     2881 acaggtcatt attcaatagg cttgaagtgt atgcctggca gaaggcggtt tggtcagact
     2941 gccttctatt taataaattg ataatcaaca tattataaga aaaattggca aataatctat
     3001 tatttgacat ttttttactt atttgttctt aaacttttgg ataatctgca atcctgcgct
     3061 gtagctttgt gtcaaacaca tacacaatga gaaagactat tctcgcactt acagcctgta
     3121 tcagtatgat actgcctgcc catgcacagc tcagcgcagg gagcctgatg ttcgtggcct
     3181 tcaatgccga cggaaccgat ggtgttgcct ttgctgctct ggaagatgta cctgccggta
     3241 ccatcattta ttttacagat aatgaatggg acggatcttc tttcaatacc ggagaaacct
     3301 actggcaatg gtcttccgga tctaccctga ctgccggttc tgtggttatc atagaaggta
     3361 ttgaccaggg tacactcaac ggaagttcac ccaaccctac tgccagtgtg ggttcctgta
     3421 gctggagtac tacttcctct ccggataaca acagcggact ctctgcttca ggtgaagcgg
     3481 tttacatgta tctggcagca tcctataata tgcctaccac tttcatttca gcaattgcca
     3541 ccggtaattt caacccgtct cttggcgaac ttaccggtac tggcctgact gaaggtgtaa
     3601 atgctattca ggtattgggt aacagcgatg ttattatttt ctataccgat gtttttggtg
     3661 cagattgcgt tgaaaccatt gcaacagaag gtaattgggt ggaagacaat ggctccggcg
     3721 accagtcaaa tgatggaaac tatcctgatt ttcctgctga tgttattgat tttctgggtt
     3781 gcatctgttc ctgcgaaaca cctgttggtt taacagcctc agccattact ccttactctg
     3841 caaccctttc ctggaccgca gttgcaggtg caacgggata cgtgatccag tacgaaaatg
     3901 aacttaccgg accgaactat cgtggaaaga tctctgacgg cactacctct gtaaatgttg
     3961 gagctggtaa gatccaaccg ggcaattctt atgtatgggg ggttaaagcc cgttgtgctg
     4021 attgctattc tgactggtct gagccttcct attttacatc accgatgcgt cttgcaggtg
     4081 gtgatgatgc cgtcgcactt attttcccga atccgtcaaa tggtcagatg accatccagc
     4141 tgcccgaaag caatgctgag accatgatca tgataactga cctttccggt aaaatggtac
     4201 tttctcagtc agtaggtgca ggtgaaactg ctgttcagct gaatatcgct gtgccaggca
     4261 tgtaccaggt gctgatctcc tctaatgaat cttctttcat ccagaaggtg atcgttcagt
     4321 gatctgttaa ttaataccag aacggccggg cttgcgcccg gccttttttt tgtgaatgca
     4381 ctgaactact ttttcctcag gtacaggtaa tccaggtaat agatcacttt aacactgaag
     4441 ttgttcccta caggtgagtt aaatactgct tccagatcat cgccgtagtt cggataaaca
     4501 taatcgccaa actgataaat ggcctgcttc cataccacat tcatttcact gcccggagca
     4561 aagaaccagg tataaacaaa gtcaatattg aaggcattga aattggcatc gttgtcagtt
     4621 ccgcccagcg cattggaggt attgtaagtg gtcgggttca acagtccgtc ttcgtcgccc
     4681 agctcataga actcctgata ggtggcgaaa ctccagtaat ggcgaagccg gaaagtaaat
     4741 cccattttta ctgttggtgt gtaagccagg ttgatcacac tgctgtattc gctgtaatca
     4801 cggcgcccta acaaaacaaa gttctcaggt atctcttccc atccgatgga aggatctgtc
     4861 cagccgatgt catttggcca tacactgacc gattgatcaa gtataagtgt gagcttatcg
     4921 ctgaaccgga ccctgggtga gaaatataca ttcactctgc tgcgcttacc ttctatgtcg
     4981 cggttcacct cttctgcaaa ccaccggaag ttggcgttca gatcaaaggc aattctttta
     5041 gaataatctg ttgatacaaa cccgcctata ttattgttga caggaaaggt atagtatctg
     5101 cccggactgc gtggctcgaa ccagtcatag gtgaccaccg gttccgttgc ccagaacatg
     5161 cctgcaaaca ggaaattctg aaaacctgct gttacattta gccaacctga gaaattgaag
     5221 aattcgttgg gatctactat gcgggtataa tccactccac cagaggtgta cagcgagttg
     5281 atatgccaga tcgggtcaaa atgattgtat tgcaactgga tgccataatt cacagtattg
     5341 ttattctgta aaaatcccag atcatttgga tcataggaag ggccctcaaa ccagtaaccc
     5401 gccgtcgcct ggaagtttcc acctatttta cctgcataca ggctgtgggc atatcccata
     5461 tcggcactgt tctgtccacc acggtattgc tgcgatgcaa tgaattttcc ttctgcaccg
     5521 taagtctgct tttcgttctt taatgcaaat ccgccgcccg ttacattggc gtcgtcaaag
     5581 cctccgtttc tggtcacgtt cgtattgatc agataggcat tgctgttatt cttcaggttc
     5641 tgatcgaaca ccacaacatt atagttggtg agcggaccgg taagctcttc tctggatgca
     5701 ccttctgcat cttcaatggt agcataggtt tcgccttcta tggcattgaa aactcctatt
     5761 cccaaaccac cctgagtcct gccggatact ttcgtggcgt tcagtaacct ggcctgctgt
     5821 gggttgttga tcaccgtctc tccatcgccc aggttgccgt aaacatccca gaatgcatat
     5881 ggagttccgc ccacccttcg agagtaaaag agattgcctt tgtcaaataa ttcaaccccc
     5941 tctgtaaaga actgccttcg ttcaacaaaa tacacctcaa aaggggtcag attgagaacc
     6001 tgattgtcac tctgcacctg gccgaagtcg ggtatcaggg tcatatcgag ggtgaatgca
     6061 tcattgaggc cgtacttcag gtcggcgcca ccattgtatg aggtggcatg acttacctga
     6121 tcattgggtc ggtcattgaa gacatcatag taggccgaca aataaggtgt aagagacaat
     6181 cttatgggag cttcaatacc attcactccc agcaatgttc cttgttgcaa tgtaaaaccc
     6241 gctatctccg gatcgatcgg actccaggtt gacttatcac ggcttctgtt cacctcccgg
     6301 aaaaactgca atccccattc ctgtgcatcc tggttgggaa aacgaatggc actgtatgga
     6361 attttcatct ccactaccca acccttttca ttgatatgag cggcactctg ccaaactgca
     6421 tcccagttga aatcttcgtt tccaccgtta atggttttgg cctccagttg tacaccacct
     6481 gcagatacga taaactcaaa tccgttgatg ccgtctttgt aggtgtctag ggcgattcca
     6541 aaaaaatctg tggcacccag ctcgtcccgc tggctgagct gctttctgat actgtcaggc
     6601 gaagggtcat acaacatagc tccaacatat agggcattgt tgtcatatac gatcttcact
     6661 tcagacttgt aggctgaggg aaccttccac tgaaactgga actgggtaaa atcagtagcg
     6721 gcatctgcct gttgccacac tgcttcatcc agaataccat caatgcgaat ggactcttcc
     6781 gtgcgaactg cctgcagggt tcttttttca ccgttcagct gactatatgc cagctcggtc
     6841 tgaaaaaaga atgccagtga taaaagatag tggtacttca tgtggttggt cttcaggcaa
     6901 acctactcaa taccccaaaa cggtacagtt gaagggttct caaatattgt taatgcttgt
     6961 taacgtctga tggcttgttc aggtatgctg agcagctatc gcatagatta ttccagctct
     7021 gcaagctcca gccagcgcat acttttttcg tccagttcat tggatacaat catcagcctt
     7081 tctgataaca caccaatagc ctgatgatcg ccggtaggtg aactcagctg ttgttcaatg
     7141 ttctgcttct cctgttccag tgcagctatg tctttttcca gcatttccat ctctctgttc
     7201 tccttgaaac tgcgtttctt tttaacggcc tctgctgctt gttttggttt gggcgcagtt
     7261 acttttttct tgtcttcctt gttctgctta tctgctgcct gtctgtactc tgtgtaattg
     7321 cctgtaaaat cgctgatcag accatctcct tcaaaaacga acaggtgatc caccagctta
     7381 tccataaagt agcggtcgtg cgatacgatc atcaggcaac cggtataatc cagcaggaat
     7441 tgctccagga tgctgagcgt caggatgtcc aggtcgttgg taggttcgtc cagtatgagg
     7501 aaattcggat tgcggatgag gatggtcatc agctgcagcc tgcgcttctc gccgccactc
     7561 agtttggata caaaagtgta ctgtgtttcc ggtgcgaaga ggaatctggt cagcagttga
     7621 gaagcactga gcttgcttcc atcggccatc ggaatgaatt cagcgatgtc tttcaccaca
     7681 tcgatcaccc gtttgtcgtc ggccacccga agaggctcct gtgagaaata tccgaatacg
     7741 atggtctcac cggcctggat cttgccactg tctgcatgct ccatgcccat gatcatattg
     7801 agaaaagtgc tctttcccac accattcttc cccacaatgc ctacccgctc acctttcttg
     7861 aatgtgtagg tgaaaccatc ggtgatgacc agtgagccaa atgccttccg caccttgacc
     7921 atctccagga tcttcccgcc tatgcggttc atttttactt ccagtttcag ctgttcctca
     7981 gggcctttac cggatgcttt ttcactcagt gtatcaaaag catccaagcg agatttgctt
     8041 tttgtggtcc gggctttcgg catgcgcctc acccattcca gttcacgccg gtacaggttt
     8101 ctggccttgt ccagttctgc ggcttccaca gcctcgcgat gtgccttctt ctccagaaaa
     8161 tcagagaaat tgccggagta gttaaagagc tttccgtctt ccagttcgat gatctgattg
     8221 cacacactgt ccagaaaata ccggtcgtgc gtaaccacca gcatggtcat atcgcgggtg
     8281 ccgatatact cttccagcca ctcgatcatg gcaacatcca ggtggttggt gggttcatcc
     8341 aatatgagca ggtcgggttc ctgtatgagc atttttgcca aagctacccg cttcgcttgt
     8401 ccacctgata gagtctccac cggctggtcc agatgatgaa tgtccagctt cgacaggatc
     8461 agacggatct tctgttcata atcccaggct tccagcgcat ccatctgcgc atgcgcttct
     8521 tccagtcgtg cggcatgcgc aggtgaatgg tcttcttcca tggcttccag acattgttca
     8581 taggcaccaa tggcctgcag caccggtgtg ttgccggaaa aaacattctc catgatgctt
     8641 tgtcccgcta tcagtttcgg ctcctgttcc agaaatccgg ttcggatctc cttgttgatg
     8701 acaacggttc ctgaatctgc gatctccgtg cccatgatga ccttcagcag ggtggacttg
     8761 ccggcgccat tgcgtgctac cag
//
LOCUS       DAOMAX010000098         8755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1146_length_8755_cov_3.473678, whole genome shotgun sequence.
ACCESSION   DAOMAX010000098 DAOMAX010000000
VERSION     DAOMAX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_8755_cov_3.473678"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..993
                     /locus_tag="PK742_06585"
     CDS             142..993
                     /locus_tag="PK742_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76292.1"
                     /translation="MLQAGMLFADIYDPMKALSIILALSLLSGCTFSSLAQQPNLSGR
                     KYTLHPCTTYWQMPGMGLTFLNDHQVCYSITYPGQLEMLGDGQYHSRITTDTLSYRIE
                     GDSIILERCEASSCLAGRPVKLQIPQPLYSVWFNAETNEKIPEDRTDTLQIYAGVDRQ
                     VILAATDITFHVWLDKTEISRTVIQEGWNEIRIPGRYFEYLFDKIYPVYELRYKIDGK
                     QYFALLHDEMTTADKSHSVQADFDPPFEPCPFHVLYPDVDLNQLHTGRSAEFRCGWFE
                     YLPVKQH"
     gene            1137..1562
                     /locus_tag="PK742_06590"
     CDS             1137..1562
                     /locus_tag="PK742_06590"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HQU76293.1"
                     /translation="MLEINFMAVLVAVVANFILGFLWYTPIFGKAWGKEMGHDPNEKP
                     KSSDMMRGLLFMVIGNFLMAYVFAHNIAAWSFVPDMENMSATGNILNSAIFTWLGFYL
                     PGELGGTVWEKKSWKLFFINTGYHLASLLVVATILILWK"
     gene            1689..2285
                     /locus_tag="PK742_06595"
     CDS             1689..2285
                     /locus_tag="PK742_06595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQU76294.1"
                     /translation="MPSVYQLKPAFQRLLTPLMRGLRSMGMTPNQLTLLAILLSAFMG
                     FSIWLHTRHFAWILVVPIGYFIRMALNALDGMMATTYEMQSKTGQVLNELGDVLSDAC
                     IILPMALIPGNNLWLTSVIALLAMLNEFAGVLAGNISGTRRYDGPMGKSDRALMIGLF
                     CLIYFFFPSISGWIVYLFCAMGILLIWSTINRLQKGIK"
     gene            2282..3226
                     /locus_tag="PK742_06600"
     CDS             2282..3226
                     /locus_tag="PK742_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQU76295.1"
                     /translation="MTIAGHSIPEEILWILAVIFGVLLSCSAIIYLLRQRRDTSLLKE
                     IHLRVWSWWRIAIGVAVVIAGPPWMGALLIAYISFVAMREMFSIGQLRGADRAGIFAA
                     YLAIPVQYFLAWHFYFEQFLYFIPMAMFIVIPVLLVLNGRMNQIGRSMSIIPAILMLT
                     AYMPSHMVLLYRLPVQPHPAGAGGLLIFLIMLTAFNDVFQFTWGKLLGKHKILPYVSP
                     NKTWEGFVGGILTTSCLGMILSFLTPLLWWQACITGFVVGLTGFLGDSIISAIKRDLR
                     LKDTDDLIPGHGGAMDRLDSILITAPFYFYLIKYFAGI"
     gene            3223..3879
                     /locus_tag="PK742_06605"
     CDS             3223..3879
                     /locus_tag="PK742_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQU76296.1"
                     /translation="MKDLLLYLFYQLIVRPWLTLIIGVRFSNRQVFDNVRQFIVVANH
                     NSHFDMVSIMAALPWRKLKNTRGVAAAEYFGKTSFSARLMNFFFHAILIERHRKEGAP
                     SAIEILDGHLKAGKSLVLFPEGTRGQAGVIADFKKGVAILLRDNPGIPFIPVYLDGFG
                     RVLPKDKWLIIPLVCKVRFGEPVYPAGQDVDEILEKVRDAIFKLKDKDERDRNVFDYK
                     "
     gene            3885..4232
                     /locus_tag="PK742_06610"
     CDS             3885..4232
                     /locus_tag="PK742_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76297.1"
                     /translation="MQTPLISIVYFAIAAMLGALGQFLYKTGADKAGGSVMSYLGNIR
                     LLGGVLCYIAVMALFVAAFKKGGSLTVLYPVYASTFIWAALLAMLAFGTPIKLVNVGG
                     MLLLIAGMYLMGK"
     gene            4240..5046
                     /locus_tag="PK742_06615"
     CDS             4240..5046
                     /locus_tag="PK742_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76298.1"
                     /translation="MNRNTSFLFLVLILLTANLNAQICYKDKAVVVIHNNELPLSEKK
                     ALVILPGLDENRNSRKHMQAFFDTTEYDLFIPDYIDHNSFENSIANFRFFAHEHQFEE
                     YGELYFISYILGTWVLNRYLEEAKPGNVAAMVYDRSPLQERAALVATENIPFLARVFV
                     GRVVEQLSERPYLTYSDTSVAKGIMIESRATNLIRFFRKKTLAIGPINWQEPDLQQDH
                     LDLMYVPLNHDQMYDHYELIGADILYFFKHHSFTSDARRTWYEWDPFTGK"
     gene            5124..6098
                     /locus_tag="PK742_06620"
     CDS             5124..6098
                     /locus_tag="PK742_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76299.1"
                     /translation="MQNRRQFLRLGGLATAASLWPFGRLSASTDGDCTLIPSETAGPF
                     PLDLSENEFYFRQDVREDRDGVQLNLKLRITGTDNCEPMSNVRVNIWHCDKDGNYSGY
                     GSETELTYLRGYQIADENGEVEFITIFPGWYPGRTCHIHFQVYVNSSYAAISQLTWEQ
                     DIKNELYAEHADIYTAGTDPVSPGSDGIFADGYAFQTATLTPNTDTGGYDAFLELSVQ
                     GSGTATGYLEMEAAKRFDLGQNFPNPADDFTSIPFTLLQEADVTLEVYALNGSRLFNS
                     TTSHYQTGSHTINLKRTDGPFPSGNHILQLRVRTNGKDHTAFRMMYWK"
     gene            6173..7051
                     /locus_tag="PK742_06625"
     CDS             6173..7051
                     /locus_tag="PK742_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76300.1"
                     /translation="MKNRWIIVIGLFLLTFQGMAQIPENRTGQLADISMKDGTRVIGK
                     LEQVTPDTVILFTAYFGDLTLLQSQIKVVRYLSEPDTDIPAGYTFVNPNASRHFFSPT
                     AIPLKKHEGYYQNFYVAFNMVNWGFTNNLSIGVAAVPFTWFMEDGMNIALTGKLGFEV
                     ADNWYLSGGFIGGFLSNVAYGGIGFGLITYGTEDNNVTLGGGYAGLAVDPGSEYEEQH
                     ETIVYNAAGMLRLSDHFALVTENWYFAVPDFLLLSYGGRYMGDKVTVDVGFVNNGDLV
                     KILPIGIPLLGVVIYL"
     gene            complement(7057..8499)
                     /locus_tag="PK742_06630"
     CDS             complement(7057..8499)
                     /locus_tag="PK742_06630"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76301.1"
                     /translation="MGRDILVVLIMMFPFLAKCQVPEDFISHRGNLITVRFNLKNEQE
                     TRELARYFEVDFDSLSRFHDIGKLVDEGWFIMKEKKNLLIIARSMDMGGKFDWLYVAV
                     PVAGASYNNAPGYPRGLPFGFNEFKDKASVFPAGDSSTVFMLYGHSDAGTVMLSGNFN
                     DWSTGATPMEKVADGWMVRVPLREGQHYYKFIVDGRWMSDPQNKHDEGDGYGGSNSVY
                     FKTNHVMRLSGYREARKVILAGSFNQWDEHMCAMQQDEQGWYLPVYLKEGTYTYKYIV
                     DKKWITDPDNPVSRADAEGNINSVLSLGQPTVFRLPGYSNARQVVLSGNFNEWDPGEL
                     LMTRTSSGWELPYVLAPGNYEYKFVVDGNWIQDPDNPLHYADNLNSNSILTVQANHRF
                     FLKGYENAGQVQLSGSFNGWAFPGYNMQRVAGGWEFNLYLPPGKSLYRFIVDGNWMAD
                     PGNNQREFNEYGDFNSIIWREMKDVYTYDK"
     gene            complement(8502..>8755)
                     /locus_tag="PK742_06635"
     CDS             complement(8502..>8755)
                     /locus_tag="PK742_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76302.1"
                     /translation="LTTLKGAGASTLEVASGQLPDLQLGGYDFDSMTFLLTDLSQLET
                     AYGTIMHGMLGYEFLSKGIMVINPRTKILRIYFYEDPIK"
BASE COUNT         2184 a         2188 c         2075 g         2308 t
ORIGIN      
        1 gggatccctc ctatctcgat ccgcgagacc cgagaagtta tggcagtccg cctgacaatc
       61 ctgccagata ctttgaacaa cggcatttcc tggcaggagt gagtgtcggg ttttgaataa
      121 tgatcccaac ctgacaaatt tatgctgcag gccggtatgt tatttgccga tatttatgat
      181 cccatgaaag cactttccat tattctggca ctctccctgc tatccggttg tacgttttct
      241 tctctggccc agcaaccaaa cctgagtggc aggaaataca ccctgcatcc ctgcaccacc
      301 tactggcaga tgcccggtat gggactgact tttctgaatg accaccaggt gtgttattcg
      361 atcacctacc cgggtcagct tgaaatgctg ggagacgggc aataccacag caggatcaca
      421 acagacactt tgagctatcg catagaagga gattccatca tactggaacg ttgcgaagcg
      481 agcagctgtc ttgccgggcg accggtgaaa cttcagatac cacaaccttt gtattccgta
      541 tggttcaatg ctgagacaaa cgaaaagata ccggaagacc ggacagacac actgcaaata
      601 tatgctggtg ttgaccggca ggtgatactg gctgcaactg atatcacctt tcatgtctgg
      661 cttgataaga ccgagatcag caggacggtc attcaggaag gatggaatga gatccggata
      721 cccggcaggt atttcgaata tctgtttgac aagatctacc cggtgtatga attgcgttat
      781 aagatcgatg ggaaacagta tttcgctttg ttacatgatg agatgaccac agcagataag
      841 tcacattcgg tacaggcaga ttttgacccg ccatttgagc catgcccctt ccatgtgttg
      901 tacccggatg ttgacctgaa tcagctgcat acaggccgat ccgcagaatt caggtgtggc
      961 tggttcgaat atttgcccgt caaacagcat taatagctta ataagtggcc ttttcaggtc
     1021 ccctgagctg atatcctgtc gaatttaaaa acctcaacaa aacttaacaa ttcgctgatg
     1081 ataaagtaca tatgaatgtg tactttcaat actttattac ccaaaaacca accactatgt
     1141 tagagatcaa ctttatggcc gtattggtgg ccgttgtagc caacttcatt cttggtttcc
     1201 tctggtacac gcccattttt ggaaaagcct ggggaaaaga aatgggccat gacccgaacg
     1261 agaagcccaa atcatctgac atgatgcggg gtctgctgtt catggtcatc ggcaacttcc
     1321 tgatggcata tgttttcgcc cacaacatag cagcctggag ctttgtacct gatatggaga
     1381 acatgtcggc caccggcaat atcctgaatt cagccatttt tacctggctt ggtttttacc
     1441 tgcccggcga gctgggtggc accgtatggg aaaagaaatc ctggaaactg ttcttcatca
     1501 acacaggata ccatctggca tctttgctgg tggttgcaac cattctgata ctttggaaat
     1561 gagaccaatg aaatgaatac tgaaagagct gtgaaatgca gctctttttt tatgcacccg
     1621 gtagtccttg cagagagtgg gcttaatatg tactttagtt cagttcgcaa acctgaaatg
     1681 atccatacat gccttctgtg tatcagttga aacccgcctt tcaacgcttg ctgaccccat
     1741 tgatgcgagg tttgcgaagc atgggtatga ccccgaatca gctgaccctg ctggccatcc
     1801 tgttgtctgc tttcatggga ttttccatct ggttacacac aagacacttt gcctggatat
     1861 tggtagtacc tatcggctat ttcatacgga tggcattgaa tgcattggat gggatgatgg
     1921 ctactaccta tgagatgcag tcaaagaccg gccaggttct taacgaactt ggtgatgtat
     1981 tgtcagatgc ctgtatcatc ctgccgatgg ccctgatacc cggcaacaac ctgtggctga
     2041 catctgtcat tgccctgctg gccatgctga acgaatttgc cggagtgctt gcgggaaata
     2101 tcagcggaac tagaaggtat gacggcccca tgggcaagag cgacagggcg ttgatgatcg
     2161 gattgttctg cctgatctat ttctttttcc cttccatttc cggatggatc gtttacctgt
     2221 tctgcgccat gggcatacta ctgatctgga gtacgatcaa tcgccttcaa aaagggatca
     2281 aatgaccatt gccggccatt ctattcctga agagatcctc tggatactgg ctgtcatctt
     2341 tggggtgctg ctcagctgct ctgccatcat ctacctgctc agacaacgcc gcgatacctc
     2401 gttactgaaa gagatccacc tgcgtgtctg gtcctggtgg cgaattgcca ttggtgtagc
     2461 agtggtcatt gcaggtccgc cgtggatggg agccctgctc attgcctaca tttcattcgt
     2521 agccatgcgg gagatgttca gcatcgggca gttgcggggt gccgacagag ccgggatatt
     2581 cgctgcctat ctggccattc cggtccagta ctttctggcc tggcatttct attttgaaca
     2641 gttcctgtat ttcatcccga tggctatgtt catcgtcata cctgtgctgc tggtcctgaa
     2701 cgggcgtatg aaccagatag gcaggtccat gtccatcatc ccggccatcc tcatgctgac
     2761 cgcctatatg cccagtcata tggtgctgct gtacagattg ccggtacaac cacatccggc
     2821 aggtgccggt ggattgctga tctttctcat catgctgacc gctttcaatg atgtgtttca
     2881 gttcacctgg ggtaagctgc tgggcaagca caagatcctt ccgtatgtga gccccaataa
     2941 gacctgggag ggatttgtgg gaggtatcct gaccacttcc tgcctgggaa tgatcctgtc
     3001 ctttcttacc ccgttactct ggtggcaggc ttgcatcacc ggcttcgtgg tcgggcttac
     3061 gggcttcctg ggtgattcga tcatttctgc catcaaaaga gatctgcgac tgaaggatac
     3121 tgatgatctc atacccggac atggaggtgc catggaccgg ctggatagca tcctgatcac
     3181 tgctcctttt tacttctatc tgatcaaata cttcgccggt atatgaaaga tctgctgtta
     3241 tacctgttct accagctgat cgtcagaccc tggctcacac tcatcatagg tgtgcgtttt
     3301 tccaaccggc aggtgtttga caatgtcaga caattcatcg tggtggccaa ccacaacagt
     3361 cactttgata tggtcagcat catggcagcc cttccgtggc gaaaactgaa aaacaccaga
     3421 ggagttgccg ctgccgaata cttcggaaag acctccttct ctgccaggct gatgaatttt
     3481 ttctttcatg ccattctgat agaacggcac cggaaagaag gtgcaccctc tgccattgag
     3541 atcctggacg gacacctgaa agcaggcaaa tcattggtat tgtttccgga gggaaccaga
     3601 ggtcaggcag gggtgatagc agattttaaa aaaggcgtcg ccattctgct cagggataat
     3661 ccgggtattc ccttcattcc tgtgtatctg gatggattcg gcagagtgct gccaaaggat
     3721 aaatggctga tcatccccct ggtttgtaaa gttcggttcg gagagccggt ctatcctgca
     3781 ggacaagatg tggatgagat cctggagaag gtcagagacg ctatcttcaa actgaaagac
     3841 aaagatgaaa gggacagaaa cgtatttgat tataaatgat atttatgcag acaccattga
     3901 tctctatcgt ctattttgcc attgccgcca tgctgggtgc actcggacaa ttcctgtata
     3961 agaccggcgc cgacaaggcc ggcggttcgg tcatgagtta cctgggtaac atccggttgc
     4021 tgggaggtgt actttgctac atcgcggtca tggcactgtt tgttgcagcc tttaaaaaag
     4081 gtggatctct cactgtcctg tatcctgttt atgccagtac cttcatctgg gctgccctgc
     4141 tggccatgct ggcttttggt acaccgatca agctcgtcaa tgtgggcgga atgctgctgc
     4201 tcattgcagg tatgtatctg atgggaaaat aacatacgga tgaacagaaa tacaagcttt
     4261 ctttttcttg tactcattct tctgacagca aatttgaacg cccagatctg ttacaaagac
     4321 aaggcagtgg tggtcatcca caacaacgaa ttgccattat cagagaaaaa ggccctggtc
     4381 atactacccg gactggacga gaaccgcaac tccagaaaac atatgcaggc cttctttgac
     4441 accacggagt atgacctgtt cattccggat tatattgacc ataattcctt cgaaaattcc
     4501 atcgccaact tcagattctt cgcccatgaa caccagtttg aagagtatgg agaattatat
     4561 ttcatctcct acatcctggg cacctgggtg ttgaaccgct acctcgaaga agcgaagccc
     4621 ggcaatgttg cggccatggt gtatgaccgc agtccgctgc aggaaagagc agcactcgtg
     4681 gccacagaga atattccatt tctggcgagg gtcttcgttg gacgggtagt ggaacagctg
     4741 agtgaaagac cctatctgac ctactcagat acctcagtgg ccaaagggat catgatagaa
     4801 agcagggcta cgaatcttat tcgtttcttc cggaagaaga ccctagcaat cggcccgatc
     4861 aactggcagg aaccggacct tcagcaagac catctggacc tcatgtatgt accgcttaat
     4921 cacgaccaga tgtacgacca ctatgaactg atcggtgcag acattctgta cttttttaaa
     4981 caccacagct tcaccagtga tgccaggaga acctggtatg aatgggaccc gttcaccgga
     5041 aaatgaatct gggcattaaa cctgacattc caaggatcgt ctaactacga tacttaccta
     5101 cctttaatgc aaaccaatat ctgatgcaaa accgccgtca atttctgcgt ttgggtggcc
     5161 tggccacagc tgcttctctc tggccttttg gtcgcctgtc tgccagcact gatggcgatt
     5221 gcaccctgat cccgtcagag actgccgggc catttccgct cgatctcagt gaaaacgaat
     5281 tctatttcag acaggatgtc cgggaagacc gggatggcgt gcagctcaat ctgaaactgc
     5341 ggataaccgg gacggataac tgtgaaccaa tgtccaatgt gcgggtgaac atctggcact
     5401 gcgataagga cggcaactat tccggatacg gaagtgaaac cgaactcacc tacctgcgtg
     5461 gataccagat cgctgatgag aatggtgagg tggagttcat taccattttt cccggatggt
     5521 atccgggccg aacctgccac attcattttc aggtctatgt caattcctct tatgcggcca
     5581 tatcacaact cacctgggaa caggacatca agaatgaatt atatgctgag catgccgata
     5641 tctataccgc aggaaccgac ccggtctccc cgggttcaga tggaattttc gccgacggat
     5701 atgctttcca gacagcaacc ctgacaccca ataccgatac cggtggatac gacgcctttc
     5761 ttgagctgag tgtgcaaggc agcggaacgg ctaccggtta tctggaaatg gaagcagcta
     5821 aacgttttga tctgggacag aattttccca accctgccga tgatttcacc agcattccgt
     5881 tcacgctctt acaggaagca gatgtaaccc tggaagtcta tgccttgaat ggcagccgcc
     5941 tgttcaacag cacaacttca cattaccaaa caggttcaca taccatcaac ctgaaacgta
     6001 ctgacggacc ttttccgtct ggaaatcata tcctgcaact gagggttcgc accaacggca
     6061 aagaccatac agcattccgg atgatgtact ggaaatagac ctgacatttg ttccttatgg
     6121 tcgtgtgacc tgtgtcattt tagttctggt catggtggac tagtttacct gtatgaaaaa
     6181 caggtggatc atagtcatag gactgtttct cctgacattt caggggatgg cacagattcc
     6241 ggagaaccgg acgggtcaac tggccgatat cagcatgaaa gacggaaccc gcgtcatcgg
     6301 gaaactggaa caggtaacac cggatacggt gatacttttt actgcatatt ttggtgatct
     6361 gaccttgttg caatctcaga tcaaggttgt cagatatctg agtgaaccgg atacggacat
     6421 accggcagga tacacttttg tgaatcccaa tgcatcgagg cattttttca gcccgacagc
     6481 catcccgttg aagaagcatg aagggtatta ccagaacttc tatgtggcct tcaacatggt
     6541 caactggggt ttcaccaaca acctctccat tggggtagca gcggtgcctt tcacctggtt
     6601 catggaagat ggaatgaata tcgccctgac cggtaagctg ggctttgaag tagcggataa
     6661 ctggtatctg tcaggcgggt tcattggagg cttcttatcc aatgtcgcat acggtggcat
     6721 cggtttcggg ctgatcacct atggaacgga agacaacaat gtgaccctgg gtggcggata
     6781 tgccggtctg gcggtggatc ccggatccga atatgaagaa cagcatgaaa ccatcgtcta
     6841 taacgcagcc ggtatgctgc ggttgagtga tcactttgct ttggtgaccg agaactggta
     6901 ctttgctgtt cctgattttc tgctgctgag ttatggcgga cgctacatgg gcgataaggt
     6961 gaccgtggat gtgggttttg tcaacaacgg agatctggta aagatccttc caataggtat
     7021 tccgttgctg ggtgtggtga tctatttgta ggcggcttat ttgtcataag tatatacatc
     7081 tttcatctcc cgccagatga tgctgttgaa atcgccgtat tcattgaact cccgctggtt
     7141 attaccggga tcggccatcc agttgccgtc cactataaaa cggtaaagag attttcccgg
     7201 aggcagatac aaattaaact cccatccacc tgctaccctt tgcatattat atcccgggaa
     7261 agcccagcca ttaaaactac cactcaactg cacttgtccg gcattttcat accctttcag
     7321 aaagaagcgg tgattagcct ggacagtaag tatcgagttg ctgttcaggt tatcagcata
     7381 atgcaacgga ttatcagggt cctgtatcca gtttccatcc accacaaatt tatattcata
     7441 gttacccggt gccagaacat atggcaattc ccagccagag gaagtgcggg tcatcagaag
     7501 ctcgccggga tcccattcgt tgaaattgcc actaagtacc acctgcctgg catttgaata
     7561 acccggaaga cgaaaaacag taggctgccc cagactcaga acggaattga tatttccttc
     7621 agcatcagct ctggatacag gattgtcagg gtcagtgatc cactttttat ctactatata
     7681 cttatatgta taagtcccct ccttcagata aaccggcaaa taccaacctt gttcgtcctg
     7741 ttgcatagca cacatatgct catcccactg gttgaagctg cccgccagta tgacctttct
     7801 ggcttcccgg tacccactca gtctcatcac atgattggtc ttgaagtaca cactgttgct
     7861 tcctccgtaa ccatcgcctt catcatgctt gttctgcgga tcactcatcc atcgtccatc
     7921 cacaatgaac ttgtagtagt gctgcccttc ccggagtggt accctgacca tccagccatc
     7981 agcaaccttc tccattggcg tggcacctgt gctccagtca ttgaagttgc cactgagcat
     8041 gaccgttccg gcatcactat gtccgtatag catgaagacc gtgctgctat cgccggcagg
     8101 aaagacactg gccttgtctt tgaattcatt gaaaccaaaa ggcaaccctc tgggataccc
     8161 cggagcattg ttgtatgaag cgcctgcaac aggcacagcc acatacaacc aatcaaactt
     8221 tccgcccata tccatagacc tggctatgat gagcagattt tttttctctt tcatgatgaa
     8281 ccagccttca tcaaccagtt taccgatatc atgaaatctg gacaaagaat caaaatccac
     8341 ctcaaaatac ctggccagtt cacgagtctc ctgctcattc ttcaggttga agcgaacagt
     8401 gatcaggttt ccacgatggg aaataaaatc ttccggaacc tgacatttgg ccaggaaagg
     8461 gaacatcatg atcagaacaa ccaatatatc tctgcccatt atcattttat cggatcttcg
     8521 taaaaatata tacgtaatat tttagttctc ggattgatga ccataattcc cttcgacagg
     8581 aattcataac caagcatacc atgcatgatg gttccgtagg ctgtttccaa ctggctcaga
     8641 tcggtcagta aaaaggtcat ggagtcaaaa tcatatcccc ccagctgcag atcaggcaac
     8701 tgtccgctgg ctacctccag ggtgctcgct cctgctcctt tcaaggtggt caggt
//
LOCUS       DAOMAX010000099         8727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1160_length_8727_cov_4.448455, whole genome shotgun sequence.
ACCESSION   DAOMAX010000099 DAOMAX010000000
VERSION     DAOMAX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_8727_cov_4.448455"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="PK742_06640"
     CDS             <1..1366
                     /locus_tag="PK742_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76303.1"
                     /translation="AQLSTEEGSSTISDKEGNLLFYTNTGFIYNTMNEPIHEDPIVGY
                     SYDPGNPSQGNQVMEWALLLPDPINQDTLLYLLTNASTLSYTKINIELNDGKGGLVDS
                     AINVLIGDSAVPMDTKLTSVRHANGRDWWIFMRSYCTAEVQFFQKWLLTPSGFQGPDY
                     IELSDWVEEEGYQFGNMFFSQNGDRLIVIEETGFEVYNFDRCSGELEQIGIIEDIPVT
                     RLWYGALSASGQKLYIIYHNFIEGSTENNSGILQYDLGFLPDSYAVKESESLVFEEDY
                     NNYGIWSIKYEPITDNMYFTLLNTTDTLYSHNMYLHAITEPELPGLLCNVQENYLQLA
                     FEPGGMRTLGGLPNMPNYALGALAGSPCDTLNETNSVAEVTSPPFKAYPNPVQDHLYI
                     DNPLHTTCQAVVFNAMGQEVEGFVLQPGGLTVSTTSWPTGIYQLVLESHSRILEKINL
                     VKLE"
     gene            1406..2146
                     /locus_tag="PK742_06645"
     CDS             1406..2146
                     /locus_tag="PK742_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQU76304.1"
                     /translation="MKTYTLLFLLPLLAAAPAKDDLFTPLNEALSISRYEVSNFEYRQ
                     FLDDIKTSDPDLYRFCMYDSTAWQNRFGYAYMEPMTTHYHSHPAYDQYPVVNITPAAA
                     TAYCEWLTDKIGKEQYRFRLPTEKEWMEASAAHTETGLPYAQADLIDHKKKKDVPLAN
                     VKVIKDGESNFMSDDAFLTAPVESFQPNDIGLFNMSGNVAEMTLDGYLKGGSWYDPAE
                     ECRVSKRQLYQTPDPRVGFRVIREKRSE"
     gene            2168..2980
                     /locus_tag="PK742_06650"
     CDS             2168..2980
                     /locus_tag="PK742_06650"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQU76305.1"
                     /translation="MSGSFKAAVNGTWQRWRFQWSADDALVFRWYYRFFYRPKAGSIQ
                     SFLDAYSKQTKNFRVIQVGANDGITHDLIHKFIKRDQWQGVLLEPQLAVFARLQQLYH
                     KNRQITPLRAAIGEQDGEASLYKIAFSQARWATGLASFNKEVILQAFASGYVENKALA
                     EGVDIPAAETDRITAESIPVISPATLIEQYHLDHFHLLQIDTEGFDLEVIRLFAAQTT
                     LPDAVIFENAHLDDEQRETARKILDEIGLQCRDFGKDTLAFRPVLQPLVPGA"
     gene            2977..4017
                     /locus_tag="PK742_06655"
     CDS             2977..4017
                     /locus_tag="PK742_06655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQU76306.1"
                     /translation="MKIALIHYRLILRGGLERRLINYSQWLQEQGHEVHIIVAKSSGE
                     VTLPEGVQLHILSPGLMPKVFRQRYFDHLLGRFMRHNHFDKSLSLMRTSHQDAVLCPG
                     NHLGFLKANGRRARTLSDREQIRADQLAFQRSKVVLAASKMMANEAIGWYHTPAEKVH
                     ILHPPVNDHLFHPELKQQQAVLRERYGLPADKRLFLFVSSSHKRKGLPLLLRCFAQAT
                     DTDSVLVIAGLPKVLSDLPNVIDLQFIPQMEELYAAVDFTIHPATYEPFGQIVSESLC
                     CGTPVIVSDMVGAAEIIGNKEGLVIPLADEKLWLDTIRTIRQEAFHIAPGVAERKGLL
                     VSQHMERMLDVI"
     gene            4084..5232
                     /locus_tag="PK742_06660"
     CDS             4084..5232
                     /locus_tag="PK742_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76307.1"
                     /translation="MKKRSPVQEIILSSFLLISFMSQAQDYEVYVSSRGENAVKKYSA
                     YGDYLGYFVAPGEGGLNGTEDILFHPDGSVLVTGFGNTTIKRYDGETGAFLGDFSSGY
                     ALATPSKMSIGPDSLIYVTQWDAVQNNIVRFDLDGNFVDEFTTEGAPNGLGHLWDADG
                     NFYVALFGTGADGVVRKFDADGNDLGDFINSGVLQGPTSIWVNAEGHMFVEDWSVGTV
                     LEYSSSGSYLGVFNSGSMNNPEGIAMTPDGLLLIGDWGQDVVHILDTLGNHLGIFAAG
                     EGLSDPNSVKIRMIDTPDTTVHVTDLPVSTWEVLPNIGCGPFTLQLHGGFSFLYVYDE
                     QGTMVDQLNVQGKQSFQWEPADLPAGMYLLVCSTSDGLQATRKILLQK"
     gene            5277..7379
                     /locus_tag="PK742_06665"
     CDS             5277..7379
                     /locus_tag="PK742_06665"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HQU76308.1"
                     /translation="MQSRPVSVHLLLPVLSLLAFGCNESLQTTSLQVASPSGNNSITF
                     MLLEEGIPAYSVYHLDSLLIDTSLLGFDLEKYGHIGFGMEVLRTERDSEDDTWEQPWG
                     EEHYVHNHYNELLVELMQPEYPYRRLNIYFRAYDDGIAFRYEFPRQDDDMDTVVILDE
                     QTQFQLTEDPTVWWTPGDWDIYEHLYNTTKFSKIDALSKQNHPNLAQTYIPQNAVNTP
                     VTMRLQDGVHLTFHEAHLEDYAGMTLAVDNENLLMTSALVGSDRLGYKVKRAIPFETP
                     WRTVQISDDAIGLLNSRMILNLNPPSRIKDISWFTPMKYAGIWWEMHLDKSTWDMASG
                     RHGATTEHAMELIDFAAANGLKGVLVEGWNTGWEHWIGFEDREGVFDFVTPYADYDLD
                     SVVAYGKSKGVDIIMHHETSAAVRTYDQQLDTAFQLMQSLDLHMVKTGYVGKIIPAGE
                     YHHGQYMVNHYRRVVETAAKYHIAIDAHEPIKDTGERRTWPNFVSREGMRGQEFNAWA
                     SDGGNPPEHLPIVAYTRMMAGPFDFTPGVFNLSLKPYKQHNQINTTLAQQLALYVVIY
                     SPVQMACDLPENYAHPAFQFIRDVGVDWEQSIPLNGEVGNWITMAREERGTGNWFVGA
                     ITDENARDVQVDFSFLPAGTSFAATVYRDGKTAHYRKDPYSLTIETMTITPETNLTFH
                     LAPGGGLAISLMKETAPE"
     gene            7457..>8727
                     /locus_tag="PK742_06670"
     CDS             7457..>8727
                     /locus_tag="PK742_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQU76309.1"
                     /translation="MDVEILSRIQFAFTVAFHYIYPPLSIGLGLFLVISEGMYLRTGD
                     KLYENLTKFWTKIFALTFSIGVATGIVMEFEFGTNWATYSRYVGDVFGSALAAEGIFA
                     FALESGFLGILLFGWNRVSKRMHFISTVGVWLGSMFSAVWIVVANSWQQTPAGFHIVG
                     EGMEARAEITDFWAMVFNPSSADRLTHVWIGAFLAGAFLVLSVHAYYLLKKKHVEISK
                     KGFGIALIMAVVFSLAQLFTGHRSAHGVSENQPAKLAAMEGHYEAFAPADMYLFGWVN
                     EEKEETSGLALPNGLSWLIHLDGDEEVTGLDAFAEEDRPGAVNAVFQFYHLMVAIGIA
                     LILLSVTGAIYWKRRKLYETSWLLQLFAWSVILPQLANQFGWYTAEMGRQPWVVYGLL
                     RTSDALSKAVTANQVWFSLVLFTFIYIVLFLL"
BASE COUNT         2213 a         2127 c         2233 g         2154 t
ORIGIN      
        1 ggcacaatta tctactgaag aagggtcgag tactatttct gataaggaag ggaatctttt
       61 gttttacaca aatacaggtt ttatttataa cactatgaat gaacctattc acgaagatcc
      121 tattgttggc tattcttatg accctggaaa tcccagtcag ggaaatcaag tcatggaatg
      181 ggcattacta ttgcctgatc ccattaatca agatacattg ctttatttgt taactaatgc
      241 ttcaactcta agctatacaa agattaatat agagctcaat gatggtaaag gaggtttggt
      301 cgattcggca ataaatgttc tcattggtga ttctgcggta ccaatggata caaaacttac
      361 ttctgtgagg catgcaaatg ggagagactg gtggatattt atgaggtctt attgcacagc
      421 agaggttcaa ttttttcaaa agtggctgtt gaccccctca ggttttcaag gacccgacta
      481 tattgaactt tctgattggg ttgaagagga gggatatcaa tttggcaata tgttctttag
      541 tcagaatggt gacaggttga tagtcattga agagactgga tttgaagtgt ataattttga
      601 cagatgtagc ggagagcttg aacaaattgg aataatcgag gatatacccg taactcgctt
      661 gtggtatggg gctttaagtg caagtggtca aaaactttac attatatatc acaattttat
      721 cgaggggtca actgaaaaca actcaggcat ccttcagtat gatcttggct ttttacctga
      781 tagttatgct gtaaaagaga gcgaatcact tgtttttgag gaagactata ataactatgg
      841 tatttggtct ataaagtatg agcctattac ggataatatg tattttacat tattaaatac
      901 tacggatact ttgtactcac acaatatgta tttacatgcc atcactgaac cggaattacc
      961 aggtctttta tgcaatgtac aggagaacta tctgcaattg gctttcgaac caggtggtat
     1021 gcgtacctta gggggcctac ccaacatgcc caactacgcc ctcggtgcac ttgcgggtag
     1081 cccttgcgat acgttaaatg aaaccaactc tgttgcagaa gtaacatccc ctcctttcaa
     1141 agcctacccc aacccggtgc aggatcattt gtacatagac aacccgctgc atacaacttg
     1201 tcaggcagtg gtgttcaatg ccatggggca ggaagtggag ggttttgtat tgcagcccgg
     1261 tgggctaact gtcagtacca cctcctggcc aacgggtata taccagcttg ttcttgaatc
     1321 acacagcagg atattagaaa aaataaacct ggtaaaactg gaatgaatca ttgcaatttt
     1381 tctaagttca cctcgttact cttcaatgaa aacatatact ctcctattcc tcctgccctt
     1441 gctggcagct gctcccgcaa aagacgacct gttcacacca ctgaatgagg ccttatcaat
     1501 cagcagatac gaggtcagca attttgaata ccgccagttc ctggatgata tcaaaacctc
     1561 tgaccccgac ctgtaccgct tttgcatgta tgacagtacc gcctggcaga accgtttcgg
     1621 gtatgcctat atggaaccta tgaccacaca ttatcatagt catccggcct atgatcagta
     1681 cccggtggtt aacatcacac cggctgctgc aactgcatac tgcgaatggc tgacggataa
     1741 gataggcaag gaacaatatc gttttcgact gccaacagaa aaggaatgga tggaagccag
     1801 tgcagctcat acagaaaccg gactgcccta tgcacaggct gatctgatcg atcacaaaaa
     1861 gaaaaaggat gttccactgg ccaatgtcaa agtgatcaaa gatggtgaaa gcaacttcat
     1921 gtcagatgat gcatttctca cagcaccggt tgaaagtttc caacctaatg atatcggctt
     1981 attcaatatg agtggcaatg tggcagaaat gaccctggat ggttacctca aaggcggcag
     2041 ctggtatgac ccggcagaag aatgcagggt cagcaaacgg cagctgtatc agacccccga
     2101 tccacgagtg ggctttcggg tgatccgtga aaagcgatct gaataagtcg ggtatcttta
     2161 cggccgtatg agcggctctt tcaaggcagc ggtcaacggc acctggcagc gatggcggtt
     2221 tcagtggagt gcagatgatg ccctcgtctt tcgttggtat tatcgttttt tctatcgccc
     2281 gaaggccggc agcatccagt cctttctgga tgcctacagc aaacagacca aaaatttccg
     2341 ggtcatacag gtaggtgcca acgatggcat tacccatgat cttattcata agttcatcaa
     2401 gcgtgaccag tggcaggggg tcttgctgga gccacagttg gcagtatttg caagattgca
     2461 gcagctctat cataaaaaca ggcagatcac tccgctcagg gctgctatcg gcgaacagga
     2521 cggggaagca tctttataca agatcgcttt cagccaggcc cgctgggcca ccggactggc
     2581 cagtttcaac aaagaggtca ttctgcaggc atttgcttcc ggctatgtgg agaataaagc
     2641 gctggcagaa ggcgtggata ttcctgctgc tgaaaccgac cgcatcacag cagaatccat
     2701 tccggtcatc tcgccggcca ctctgatcga acagtatcat ctggatcatt ttcatctgtt
     2761 gcagatcgat acggagggct ttgatctgga ggtgatcaga ctgttcgcag cccagacaac
     2821 attgcccgat gcggtcatct ttgagaacgc tcatttagac gatgaacagc gggaaacagc
     2881 ccggaaaatc ctggacgaaa tcggactgca atgccgagat tttggtaagg atacattggc
     2941 cttcagacct gtacttcaac cactcgttcc cggcgcatga agatcgccct gatacattac
     3001 cggctcatcc ttcgtggcgg actcgaacga cgcctgatca attacagtca gtggttgcag
     3061 gaacagggac atgaggtgca catcatcgtg gcgaaatcat cgggagaagt aacacttcct
     3121 gaaggagtgc agctacatat cctatcgccc ggactgatgc ccaaggtatt ccggcaacgc
     3181 tatttcgatc atcttctggg aagattcatg cgtcacaacc atttcgacaa aagcctgtcg
     3241 ctcatgcgca cctctcacca ggatgcggtg ctgtgccccg gcaatcacct gggctttctg
     3301 aaagccaacg gcaggagggc ccgtaccttg tcagaccgcg agcagatccg tgccgaccag
     3361 ctggctttcc agcgcagcaa ggtggtgctg gctgcttcta agatgatggc caatgaagcc
     3421 atcggatggt atcatactcc agcagaaaaa gtgcacatcc tgcatccgcc tgtgaacgat
     3481 catttgtttc atccggaact gaaacaacaa caggctgtcc ttcgtgaacg atacggttta
     3541 cctgccgaca agcgactgtt cctgttcgtt tccagttcgc ataaaagaaa aggactacct
     3601 cttctgcttc gatgcttcgc ccaggcaaca gataccgatt cggtactggt catcgcagga
     3661 ttgccaaaag tgctgtcgga ccttcccaat gtgatcgacc tgcagttcat cccacaaatg
     3721 gaagaactgt atgccgccgt cgatttcacc atccaccctg ccacctatga accgttcggg
     3781 cagatcgtgt ctgaatcgct ttgttgcggt acaccggtca tcgtttcaga catggtggga
     3841 gctgctgaga tcatcggcaa caaagaaggg ctggtcattc cgctggctga tgaaaaacta
     3901 tggctcgata ccatacggac catcagacaa gaagcatttc acatcgcacc gggtgtggcc
     3961 gagagaaaag gtctgctggt ttcgcagcat atggaacgta tgctggatgt catttaacac
     4021 ttcttgagaa aggcctgacg gtatcatggc agtgggttgc tcgcatgacc ctactttcgg
     4081 tgcatgaaaa aacgttcgcc ggtccaggag atcattctgt ctagcttcct gctgatatcc
     4141 ttcatgtcgc aagcgcagga ctatgaagtg tatgtgagca gccgcggaga gaatgccgtc
     4201 aaaaaatact ccgcctatgg cgattatctc ggctactttg tggctccggg tgaaggtggg
     4261 ctcaacggca cggaagacat tctttttcat cctgatggtt cagtacttgt aacaggattc
     4321 ggtaatacca ccatcaaaag atacgatggt gagacagggg cttttctggg cgatttcagt
     4381 tcgggatatg ctcttgccac tccatcgaaa atgagcatcg ggcccgacag cctgatctac
     4441 gtgacccaat gggatgccgt gcagaacaac atcgttcgtt tcgatctgga cggaaatttt
     4501 gtggatgaat tcaccaccga aggagctccc aacgggcttg gtcacctctg ggatgccgat
     4561 ggtaattttt atgtggcatt gttcggtaca ggtgccgatg gtgtggtcag aaaatttgat
     4621 gccgatggca atgacctggg tgactttatc aactcaggtg tgctgcaagg acctaccagc
     4681 atctgggtga atgccgaagg acacatgttc gtggaagact ggagtgtagg tacggtgctg
     4741 gaatacagca gcagtggcag ctacctgggt gtgttcaatt caggttccat gaacaatccg
     4801 gaaggtattg cgatgacacc cgacggcctg ctgctcattg gcgactgggg acaggatgtg
     4861 gtacatattc tggatacact cggaaatcac ctgggcattt ttgctgccgg cgaaggattg
     4921 tcagatccca acagcgttaa gatcagaatg atcgataccc ctgatacaac ggtgcatgta
     4981 accgatctgc ctgtgtccac atgggaggtc cttccgaaca ttggctgtgg tccgttcact
     5041 ttgcagctgc atgggggttt ttcatttctg tatgtctatg acgaacaggg taccatggtg
     5101 gatcagctga acgtgcaggg caaacaaagt tttcaatggg aacctgctga tcttcctgcc
     5161 ggtatgtatc tgcttgtgtg cagcacatca gacggacttc aggctacccg gaagatcctg
     5221 ctgcagaaat agccctccgt ctaaaagcgg tttaggttgt acttttggaa aacactatgc
     5281 aatccagacc ggtatctgtt cacttgttac tacctgtact gagcctgctg gccttcggct
     5341 gtaatgagag tttgcaaacc acctcactgc aggtagcctc gccctccgga aacaacagca
     5401 tcactttcat gctgctggaa gaaggtatcc ccgcctattc agtctatcac ctcgacagtc
     5461 tgctcataga tacctccctg ctcggatttg acctggagaa atacggacat atcggctttg
     5521 gtatggaagt gctgcgcacg gagcgtgaca gcgaagatga tacctgggag caaccgtggg
     5581 gggaagaaca ctatgtgcac aaccactaca acgagctgct ggtggaactc atgcagccgg
     5641 aatatccata cagaagatta aatatttatt ttcgtgcata cgatgatggc atcgccttcc
     5701 ggtatgaatt cccccggcag gatgatgaca tggacaccgt ggtcattctg gatgaacaga
     5761 ctcagttcca gctgacggaa gatcccactg tgtggtggac ccccggcgac tgggacatat
     5821 acgaacacct gtataacacg acgaagtttt cgaagataga tgcgctgagc aaacagaacc
     5881 atcccaacct ggcccagacc tatattccgc agaatgcggt taacacgcct gtcaccatgc
     5941 gcctgcagga tggtgtgcac ctgacctttc acgaagctca cctggaagat tatgccggca
     6001 tgaccctggc tgttgacaat gagaacctgc tcatgaccag tgccctggtg ggatcagaca
     6061 gactgggtta caaggtcaaa cgtgccatac cctttgaaac accctggcgc actgttcaga
     6121 tcagtgatga tgccatcggg ttgctgaatt ccagaatgat cctcaacctt aaccccccca
     6181 gcagaataaa agacatatcg tggttcacac ccatgaaata tgccggtatc tggtgggaaa
     6241 tgcatctgga caaatccacc tgggatatgg ccagcggccg gcatggtgcc accaccgaac
     6301 acgccatgga actcatagat tttgccgctg ccaacggact gaaaggcgtg ctggtggagg
     6361 gatggaatac cggctgggaa cactggatcg gttttgaaga ccgggaaggc gtgtttgatt
     6421 tcgtgacccc ctatgccgat tatgacctgg actctgtagt ggcatatgga aagtcaaaag
     6481 gtgtggacat catcatgcac cacgagacct ctgcggctgt gcgcacctat gatcagcaac
     6541 tggataccgc atttcaactg atgcagtcgc tggatctgca catggtcaaa acaggatatg
     6601 taggcaagat cattccggca ggcgaatacc accatggtca gtacatggtg aatcactatc
     6661 gcagggtagt ggaaacagca gcgaaatatc acattgcgat agatgcacac gaacccatca
     6721 aagacaccgg tgagcgccgc acctggccca atttcgtttc gcgggaaggc atgcgtggac
     6781 aggaattcaa tgcctgggca tcagatggcg gcaatccgcc ggaacactta ccgatagtag
     6841 cctatacccg catgatggca ggcccctttg atttcacccc cggtgtattc aacctgtcgc
     6901 tcaagcccta caaacaacac aaccagatca acacgacact ggcgcagcaa ctggcactct
     6961 atgtggtcat ttacagtccc gtacagatgg cctgcgatct gcccgagaac tatgcgcatc
     7021 ctgccttcca gttcatccgt gacgtgggtg ttgactggga acaaagcata ccgctcaatg
     7081 gtgaagtggg caactggatc accatggcga gagaagaaag gggtaccggc aactggttcg
     7141 tgggggccat caccgatgag aatgcccgcg atgtgcaggt ggacttcagt tttctgccgg
     7201 caggcacctc ctttgccgca accgtttatc gcgatgggaa gaccgcacat taccggaaag
     7261 atccgtacag cctgaccata gagaccatga ccatcacacc ggaaaccaac ctgacctttc
     7321 acctggcacc gggcggcgga ctggccatca gcctgatgaa agaaacagca ccggaatagt
     7381 aactgtggtc accgcagcaa cacgaaccac gcagctactt ttgtggttgt attttcaggc
     7441 actcaaatga caacccatgg acgttgagat actctcacgg attcagtttg ctttcactgt
     7501 tgcatttcac tacatctatc cgccactcag tatcgggctg ggtttatttc tggtcatatc
     7561 tgaagggatg tatctgcgaa cgggcgacaa gctgtatgaa aacctgacga agttctggac
     7621 caagatcttc gccctgacct tcagtatcgg tgtggctacc ggcattgtga tggaatttga
     7681 attcggtacc aactgggcaa cctattcccg ttatgtgggc gatgtgttcg gcagtgccct
     7741 ggcagcggag ggcatctttg ctttcgcatt ggaaagcgga tttcttggca tcctgttgtt
     7801 cggatggaac cgggtgagca agcgcatgca cttcatttca accgtgggag tctggctggg
     7861 atccatgttc agtgccgtat ggatcgtggt agccaacagc tggcagcaga cacctgcagg
     7921 ttttcatatt gtgggggaag gaatggaagc acgtgcagag atcactgact tttgggcaat
     7981 ggtattcaac ccctccagcg cagatcgcct gacgcatgtc tggatcggag ctttcctggc
     8041 gggagcattc ctggtgctga gtgtacatgc ctattacctg ctgaagaaaa aacatgtgga
     8101 gatatcgaaa aaaggtttcg gtattgcact gatcatggcg gttgtttttt ccctggctca
     8161 gttgttcacg gggcaccggt ctgcccatgg cgtatctgag aatcagcccg caaaactcgc
     8221 agccatggaa gggcactatg aagcatttgc tcctgccgac atgtacctgt tcggctgggt
     8281 caatgaagaa aaagaggaga ccagtggttt ggctttgccg aacggactga gctggctcat
     8341 tcatctggac ggtgatgagg aagtgaccgg actcgatgcc tttgcggaag aggatcggcc
     8401 cggtgcggtc aatgcggtct ttcagttcta ccatctgatg gtggccatcg gaatcgccct
     8461 gatcctgttg tctgtcacag gtgccattta ctggaaacgg agaaagctgt atgagaccag
     8521 ttggttgctc cagctgtttg cctggtcggt catcctgcct cagctggcca atcagttcgg
     8581 ctggtatacg gctgagatgg gcagacaacc ctgggtcgtg tatggactgc tgcgcacgtc
     8641 tgatgcactg tcgaaagctg tcactgccaa ccaggtctgg ttctctctcg tcctgttcac
     8701 atttatttat atagtgctgt tcctgct
//
LOCUS       DAOMAX010000100         8594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1187_length_8594_cov_4.852676, whole genome shotgun sequence.
ACCESSION   DAOMAX010000100 DAOMAX010000000
VERSION     DAOMAX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1187_length_8594_cov_4.852676"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..947
                     /locus_tag="PK742_06675"
     CDS             270..947
                     /locus_tag="PK742_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQU76310.1"
                     /translation="MLGAYLIGIVFAIAGMIASGVLKSKFRKYSQIPVAGGLTGRDIA
                     ERMLRENGIDDVQVISVAGQLTDHYNPQNKTVNLSEGVYNSNSIAAAAVAAHECGHAV
                     QHAKGYAWLELRSQLVPVVSIASQWVMWVILAGILLINTFPSLLLIGIILFGATTLFS
                     FVTLPVEFDASRRAIAWLGQSSVAGTMEMSKAKDALRWAASTYVVAALGSLATLLYYI
                     MIYTSRR"
     gene            1109..1552
                     /gene="rplM"
                     /locus_tag="PK742_06680"
     CDS             1109..1552
                     /gene="rplM"
                     /locus_tag="PK742_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQU76311.1"
                     /translation="MDTLSFKTVSANNATVTRKWLIVDAEGQTLGRLSTRVAAILRGK
                     NKPYFTPHVDCGDYVIIINAEKVKLTGSKMLDKVIQHYTGHPGGRKVTNPRTLLRNNP
                     EKLLERSIKGMLPKNKLGSAMYKKLFVYAGSEHPHVAQKPETLTF"
     gene            1555..1941
                     /gene="rpsI"
                     /locus_tag="PK742_06685"
     CDS             1555..1941
                     /gene="rpsI"
                     /locus_tag="PK742_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQU76312.1"
                     /translation="MDKLNAVGRRKAAVARVYIDKGKGNITVNGKDFKEYFCVPFMQD
                     QVLMPLQALEMTGDFDIRVNVKGGGIKGQAEATRLSIARALVKVNEEWKPALKAKGLL
                     TRDSREVERKKYGLRKARRATQFSKR"
     gene            1963..2826
                     /gene="rpsB"
                     /locus_tag="PK742_06690"
     CDS             1963..2826
                     /gene="rpsB"
                     /locus_tag="PK742_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQU76313.1"
                     /translation="MEKITYNDLLEAGVHFGHLRKKWNPKMLPYIFMEKKGIHLIDLN
                     RTLEKLDEAANALRGIARSGKKVMFVATKKQAKEIMEEQARRVGMPFVTERWLGGMLT
                     NFTTIRKSIKKLQTIDKMLGDAGTNITKKERLVLGRERDKLEKVIGGVAQLNRLPAAL
                     LIVDIGYEHIALAEAHKLNINTIGMVDTNCDPNKVDYAIPANDDAAKSIELIIKYLTD
                     AIAEGLEERAKSKEEADEAARNEDDDDDMEVVVNIDEDEDGEKRKSRTSKGVADKKGP
                     ARRPAGRRPSR"
     gene            2858..3697
                     /gene="tsf"
                     /locus_tag="PK742_06695"
     CDS             2858..3697
                     /gene="tsf"
                     /locus_tag="PK742_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQU76314.1"
                     /translation="MSTVTISAADVNKLRQLTGAGMMDCKKALTECGGDFDAAVDWLR
                     KKGQKVSEKRADRDVNEGVAVALLNDDATSGIILALGCETDFVAKNDDYVNFARSIAE
                     LALANKPADKDALMNLDLNGMTVANAISEQVGKIGEKIEVTDYAAIDAASVVAYNHSN
                     GKVSVLIGMNKAGDAVAEIGKDVAMQIAAMNPIAIDKDGVPEDVKQREMEIGREQARA
                     EGKPEQILDKIATGKLEAYYKDNTLLNQKFVKDNSKSIRDVLAGVDKELSVTAFKRIA
                     LGA"
     gene            3767..5401
                     /locus_tag="PK742_06700"
     CDS             3767..5401
                     /locus_tag="PK742_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU76315.1"
                     /translation="MISARNITLSYGKRVLFDEVNISFTKGNCYGVIGANGAGKSTFL
                     KILSGEIEPNKGNVEITPGERMAVLRQDHSVFNDYAVLQTVMMGHSQLWKVMEERDAI
                     YAKPDFSEEDGLQAAHLEAEFGEMGGYTAESDAATLLGSLGVEESLHEMQMKDIPGSM
                     KVRVLLAQALFGNPDVLLLDEPTNDLDVETIAWLEDFLADYENIVIVVSHDRHFLDAV
                     CTHVADVDRSKIQIHTGNYTFWYETSKLAARQMSDKNKKMEEKRKELQEFIARFSANA
                     SKSRQATSRKKALEKLVIEEITPSNRKYPGIIFRPDREVGNQVLKVTNLSASNDGRSL
                     FSNCTFTVERNDKIAVFSKDHLAVTALFEMLNNNRQADSGQIEWGTTITSAYLPNDNA
                     AFFTKGMTLMDWLAQFVPANVEDADEQFLRGYLGKMLFSGDDVMKMTNVLSGGEKVRC
                     MISRMMLQNPNLLMLDEPTNHLDLESIQAFNENVAEFPGVVLLSSHDHTFMQTVANRV
                     IELTPSGIIDKLMTFDEYLNDERIHQQREEMYGVGV"
     gene            5570..6316
                     /gene="fabG"
                     /locus_tag="PK742_06705"
     CDS             5570..6316
                     /gene="fabG"
                     /locus_tag="PK742_06705"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQU76316.1"
                     /translation="MRLLENNVCLVTGASRGIGEAIALRFAENGAHVAFTYQSSEEKA
                     RILEDKLNAAGVKAKGYKSNAGSYAEAEQLIADILADFGQLDVVVNNAGITRDNLMLR
                     MSEQEWDDIMAVNLKSCFNITKHAIKPMMRAKKGSIINISSIVGLSGQAGQANYAASK
                     AGIIGFTKSVAKELGSRNIRSNAIAPGFIQTDMTDELPENLKETYFKQIPLGRFGKPE
                     EVADVAVFLASDMSGYVNGQVLSVCGALYT"
     gene            6373..8466
                     /locus_tag="PK742_06710"
     CDS             6373..8466
                     /locus_tag="PK742_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76317.1"
                     /translation="MFQLLSDFPWWAWLLCVLTGAAFAAMLYARDRKLPVSWKPWRYI
                     LAAFRFLAITIIAILLLEPYLRSTYNETLKPVAVLVEDNSASIGTALEDTTLWQQAWR
                     ELGDKLEGTYDVQRYTFGTQLTADGQADFSAPATDMSAALHSLNQLYRYANVGAVILS
                     GDGIYNTGENPLYTNIDLKAPVFTVALGDTTRQRDLRVSRVLCNNIVFTGDRFVVHAE
                     TMALLASGKQMQAYVEEVTENSSVRKAGQTVLISSDQDEPAFDFILQADQPGLHHYRV
                     KLEALDGEQNTVNNSMDFYAEVLNARQKILLIADAAHPDIAAIRSAIDQNKNYDLTVR
                     LSNEYDGDLSGYSLIILHKIPSGTGASNSILKDIQSVKTPLWIITGQLPDWDDLNALQ
                     SLVRIRGGSSKGDEVSAVPADGFNLFITEDPVMRSFSGFPPLLAPYGDYSTGTGSQIL
                     FRQQLGGARTNNPLWLFSQPGTRKTSVVCGENLWKWRLHDYLENGDHAQFDELIGKTI
                     QYLATKEEEQQFRVSASRNVFREVEPVRLDAELYNDAYELINEPDVACVIRGEDGNEY
                     PFTFSRSGQRYTLDAGYLPPGRYTVQGTTRYNDKPLQDEAAFSVVAVQLETSNTVADH
                     QLLYKLASDHGGQMFRPDQLNALADSLLQSAWAKPTLREKIQTRSVINLRWLLFLIIG
                     LLAVEWFVRKWSGGI"
BASE COUNT         2372 a         2019 c         2344 g         1859 t
ORIGIN      
        1 aaaggcagag cccgaacgcg aaggacctgt tcaaggggga gtgagagtgg gagtgtggag
       61 tgagagtggg agtgaggagt gggagtggga gtgaggagtg aggagtgaga gtggaaaaag
      121 aaaacagaca aggtagtctg aaggtgatct gagtggttta cccgttgggg aggcggatac
      181 aatttccttt cattttggtc gtctgaactt taagaaactt tttaccaccg ctttcgttat
      241 tattttcaca acaaataagt gaaccatcca tgttaggagc ctatttaatc ggtatcgttt
      301 ttgccatcgc aggtatgatc gccagtggtg tgctcaaaag caaattccgc aagtattccc
      361 agatcccggt agccggcggg ctgaccggtc gcgatatcgc agaacgcatg ctgcgggaga
      421 acggcattga cgatgttcag gtcatctcgg tggccgggca gctgacggat cattacaatc
      481 cccagaacaa gaccgtcaac ctcagcgaag gagtctataa cagcaatagt attgcagctg
      541 cagcggtagc agcccatgaa tgcgggcacg ctgtgcagca cgcgaagggc tatgcctggc
      601 tggaattgcg cagccagctg gtgccggtgg taagcatcgc ttctcagtgg gtcatgtggg
      661 tcatcctggc gggtatcctg ctcatcaata ccttcccgag cctgctgctt atcggtatca
      721 tcctgttcgg tgccaccact ttgttcagtt tcgtgaccct gccggtagag tttgatgcca
      781 gcagaagggc tatagcctgg ctgggacaaa gctctgtagc tggtaccatg gagatgagca
      841 aagccaaaga tgccctgcgt tgggccgcat ctacctatgt ggtagctgcc ctgggttctc
      901 tggccaccct gctctattat atcatgatct atacgagcag gcgctgatca tatgcgaaag
      961 aaataaatta tttccaaaat tagactgatt atcagcaggt tatgaatata gcctgctttt
     1021 ttcttttaca tttcttgcct atgcgcaggg ggtagcggta tctttgcact cccgcttcag
     1081 gcggtatgaa caaaaaaatt gtcaaacagt ggatacactc agttttaaaa cggtatcggc
     1141 caacaacgcc acggtaacca ggaaatggtt gatcgtggat gcagaaggcc agaccctggg
     1201 tcgcctcagc acccgcgtgg cggctattct ccggggcaag aacaaaccgt atttcacccc
     1261 gcacgtggac tgcggagatt atgtgatcat catcaacgct gaaaaagtga agctgaccgg
     1321 cagcaagatg ctggacaagg tcattcagca ctataccggc catcccggcg gtcgtaaggt
     1381 gaccaatccc cgtacccttc tgcgcaacaa cccggagaag ctcctcgaac gctccatcaa
     1441 gggaatgctg cccaagaaca aactgggaag cgccatgtac aagaaactat ttgtttacgc
     1501 aggttcagag catccgcatg tagctcagaa acctgaaaca ctcaccttct gattatggac
     1561 aaactgaatg cagtaggaag aagaaaagct gccgttgccc gggtctatat tgacaaaggc
     1621 aaaggcaaca ttaccgtgaa cggtaaggac ttcaaggaat atttctgtgt gcccttcatg
     1681 caggatcagg tgctcatgcc cctgcaggcc ctggagatga ccggcgattt cgacattcgt
     1741 gtgaacgtga aaggcggtgg catcaaaggc caggccgaag ctacccgttt gtctattgcc
     1801 cgtgccctcg tgaaggtcaa tgaagaatgg aagccggccc tgaaggccaa aggcctcctc
     1861 acccgcgact ccagagaagt ggaaaggaag aaatacggtc tgcgcaaagc acgtcgtgcc
     1921 acccagttct ctaaacgtta atcccgaaaa gatcagatta agatggaaaa aatcacctat
     1981 aacgatcttc tggaagcggg tgtgcacttc ggtcacctgc gcaagaagtg gaatccaaaa
     2041 atgctgccct acatcttcat ggagaagaag ggtattcacc tgatcgacct gaaccgcacc
     2101 ctggagaagc tggacgaagc tgccaacgca cttcgcggta tcgcacgaag cggtaagaag
     2161 gtcatgttcg tagctaccaa gaagcaggcg aaagagatca tggaagaaca ggcccgccgc
     2221 gtgggcatgc ctttcgtgac cgaacgctgg ctgggaggta tgctgaccaa cttcactacc
     2281 attcgcaaga gcatcaagaa actccagacc atcgataaga tgctgggaga tgccggcacc
     2341 aacatcacca aaaaagaacg cctcgtgctg ggtcgcgaac gtgataaact ggaaaaggtg
     2401 atcggtggtg tggctcagct gaacaggttg cctgcagcac tgctcatcgt ggacataggg
     2461 tacgaacaca tcgcccttgc ggaagcacat aagctgaata tcaacaccat cggtatggtg
     2521 gataccaact gcgatccgaa caaggttgac tatgccatcc ctgccaacga tgatgctgcc
     2581 aaatccatcg aactcatcat caaatacctg accgatgcca tcgctgaagg tctggaagaa
     2641 cgtgccaaga gtaaagaaga agctgatgaa gctgcccgca acgaggatga tgatgatgat
     2701 atggaagtag tggtcaatat tgacgaagac gaagatggtg aaaagcggaa aagccgcacc
     2761 agcaagggag tggccgataa gaaaggccct gcccgtcgcc ccgccggccg ccgcccttca
     2821 cgctaagatt agtttgaaca attaaaaagg agaattaatg agtactgtaa cgatcagcgc
     2881 cgccgacgtg aataaactcc gtcagttgac cggcgcaggt atgatggatt gtaaaaaagc
     2941 cctgaccgaa tgtggtggcg attttgatgc cgctgttgac tggttgcgga aaaaaggaca
     3001 aaaagtaagc gaaaagcgtg cagaccgtga tgtaaatgaa ggggtagccg tagcactgct
     3061 gaatgatgat gctaccagtg gtatcattct ggctttaggt tgtgaaaccg acttcgtagc
     3121 caagaacgat gactatgtga actttgcccg ctccattgct gaactggccc tggccaacaa
     3181 accagccgat aaagacgctc tgatgaatct ggacctgaac ggtatgaccg ttgccaacgc
     3241 catcagtgaa caggtgggta agataggaga gaagatcgaa gtaacagatt atgctgccat
     3301 tgatgccgcc agtgtggttg cctataacca cagcaacggt aaggtaagtg tactgatcgg
     3361 tatgaacaaa gccggcgatg cagttgcaga gatcggaaaa gatgtggcca tgcagatcgc
     3421 tgccatgaat cctattgcta ttgataagga tggtgtgccg gaagatgtga agcaaagaga
     3481 aatggagatc ggccgcgaac aggctcgtgc agaaggtaag ccggagcaga tcctcgacaa
     3541 gatcgctacc ggaaaactgg aagcatatta caaggacaat accctgctca accagaagtt
     3601 cgtgaaagac aacagcaagt ctatccgcga tgtactggcc ggcgttgaca aggaactcag
     3661 cgtgaccgcc ttcaagcgga tcgccctggg cgcctgatgc gcatgcagaa caatcattca
     3721 gaaacgctgc agtcttgcag cgtttctatt ttcaagaagt accatcatga tcagtgcaag
     3781 aaatatcacc ctctcttacg gcaaacgtgt gttgtttgat gaggtcaaca tcagttttac
     3841 caaaggcaac tgctatggcg tgataggcgc caacggtgcg ggcaagtcca ccttcctcaa
     3901 gatcctttca ggtgagatcg aaccgaataa aggcaacgtg gagatcactc ccggcgaacg
     3961 catggctgtg ctgcgacagg accattctgt gttcaatgac tatgccgtat tgcaaacggt
     4021 catgatggga cacagtcagc tgtggaaggt gatggaagaa cgcgatgcca tctatgccaa
     4081 gcccgatttc tctgaagagg atggactaca ggcagcccac ctggaagctg agttcggaga
     4141 gatgggagga tacaccgcag aaagtgatgc cgcaaccttg ctgggatcac tgggtgtgga
     4201 agaaagtttg catgagatgc agatgaaaga cattccggga agcatgaaag tacgggtact
     4261 cctggctcag gcactgttcg gaaatccgga tgtgctgctg ctggatgaac ccaccaacga
     4321 tctggacgta gagaccatcg cctggctgga agattttctg gcagattatg agaacatcgt
     4381 catagtggtg tctcacgacc gtcacttcct cgatgcggtc tgtacccatg tggctgatgt
     4441 ggatcgcagc aagatccaga tccataccgg taactatacc ttctggtacg agaccagtaa
     4501 actggccgcc cgacagatgt ctgacaagaa caagaagatg gaagagaagc ggaaggaact
     4561 ccaggagttc atcgcccggt tctctgccaa tgcttccaaa agccgccagg ctaccagccg
     4621 gaaaaaagca ctggagaaac tggtcattga agagatcaca ccatccaaca gaaagtatcc
     4681 tggaatcatc ttccgcccag accgcgaagt gggcaaccag gtgctgaagg tcaccaacct
     4741 gagtgcctcc aatgatggac gcagcttgtt cagcaactgc acttttaccg tagaacgcaa
     4801 tgataaaata gctgtattca gcaaggatca cctggcagtt acagcccttt ttgagatgct
     4861 gaataataat cgtcaggctg acagcggaca gatagaatgg ggtactacca tcacctctgc
     4921 ctatctgccc aacgacaatg cagccttctt caccaaaggc atgaccctga tggactggct
     4981 tgcgcagttt gtaccagcca atgtggaaga tgcagatgag cagttcctgc gaggatattt
     5041 aggtaagatg ttattcagcg gcgatgatgt catgaagatg accaacgtgc tgagcggtgg
     5101 tgagaaagtg cgctgtatga tcagccgaat gatgctgcag aaccccaacc tgctgatgct
     5161 cgatgaaccc accaaccacc tggacctgga aagtattcag gcattcaacg agaatgtggc
     5221 agaatttccc ggggtagtgc tgctgagctc acatgaccat accttcatgc aaacagttgc
     5281 caatcgtgtg atcgaactga caccttccgg catcatagac aagctcatga cctttgatga
     5341 atacctcaac gacgagcgta ttcaccagca gagggaagag atgtatggag taggagtgtg
     5401 agccttcggc agtgaggagt gaggagtgag gagtgaggag tgaggagtga ggagtgagga
     5461 gtgaggagtg gaattttggg gaagtaagtt accggtctga acgaaccacg caaaacgatc
     5521 aacgatcaac gatcaacgaa tcacgcaaaa cgaataacga ataacgaaaa tgagattact
     5581 cgaaaacaat gtatgcctgg tgaccggtgc ttcacgggga atcggagaag caatcgccct
     5641 gaggtttgca gaaaatggtg cacatgtggc gttcacttat cagtcgtcag aagagaaagc
     5701 acgtattctg gaagataaac tgaatgcagc aggtgtaaag gctaaagggt ataagtcgaa
     5761 tgccggttcc tatgcagaag ctgagcagct gatagcagat attctggccg acttcggtca
     5821 actggatgtg gtagtgaaca atgccggtat cacccgtgat aacctcatgt tgcgcatgag
     5881 tgaacaggaa tgggatgata tcatggctgt gaacctgaag agttgtttca atatcaccaa
     5941 gcatgccatc aaacctatga tgcgggcaaa gaaaggcagc atcatcaaca tctcatccat
     6001 tgtagggctg agcggtcagg ctgggcaggc caactatgct gcttccaagg caggcatcat
     6061 cggattcacc aagagtgtgg ccaaagaact gggttcccga aacatacgga gcaatgccat
     6121 cgctcccggt tttatccaaa cagatatgac cgatgagctt cctgaaaatc tgaaggaaac
     6181 ctatttcaag cagatccccc tgggccgctt tggtaaacct gaggaagtgg ccgacgtagc
     6241 cgtgttcctg gccagcgata tgtctggcta tgtgaacgga caggtattga gcgtatgcgg
     6301 cgctttgtac acctgatctg caacccggaa aatcttctag ctgaattacc gttactttag
     6361 cggtctgtca ttatgttcca gttgctatct gatttcccct ggtgggcctg gttgctgtgc
     6421 gtactaaccg gtgctgcatt tgctgccatg ctgtatgccc gcgacaggaa actgccagtc
     6481 agctggaaac cctggcgata tatactggca gcattcagat ttctggccat caccatcatc
     6541 gccatcctgc tgctggaacc ctatctgaga agcacctaca acgaaacact gaaaccggtt
     6601 gcagtgctgg tagaagacaa ctctgcttct ataggaacag ctcttgaaga taccacccta
     6661 tggcagcagg cctggcggga attaggagat aaactggagg gaacctatga cgtgcagcga
     6721 tataccttcg gtacccaact gacagccgac gggcaggccg atttttctgc tccggctacg
     6781 gatatgtcgg ccgcactcca ttcgctcaac cagctctatc gctatgcgaa tgtcggtgca
     6841 gtgatcctct cgggcgatgg catctacaac accggcgaaa atccactcta tacgaatatt
     6901 gatctcaaag ctcctgtctt tacagttgcc ctgggcgata ctacccgtca gcgagatctg
     6961 cgggtcagca gggtgttgtg caacaacatc gtttttaccg gcgatcgttt cgtagtgcat
     7021 gcagaaacca tggctttgct ggcatcaggt aagcagatgc aggcgtatgt ggaagaggtc
     7081 actgagaaca gttcggtcag aaaagcaggg caaacagtac tcatcagcag tgatcaggat
     7141 gaacctgcgt tcgattttat acttcaggct gatcagccag ggctgcatca ctatcgtgta
     7201 aaactggaag cactggatgg agagcaaaat acagtcaaca acagtatgga cttttatgca
     7261 gaggtgctga atgcacgcca gaagatcctg ctgatagccg atgcagctca tcctgatata
     7321 gcagcgatac gaagtgccat cgatcagaat aagaactacg atctgaccgt cagactatcg
     7381 aatgaatacg acggcgacct gagcggatac agcctgatca ttctgcacaa gatcccgagc
     7441 ggaacgggag ccagtaacag tattctaaaa gacatacaat cggtcaaaac tcccttgtgg
     7501 atcattaccg gacaactgcc cgattgggat gatctgaatg cactgcaatc actggtgcgc
     7561 attcgcggcg gcagcagcaa aggcgatgaa gtaagtgcag ttccagccga tggtttcaac
     7621 ctttttatta ccgaggatcc ggtcatgaga tccttctccg gatttccacc actgctggca
     7681 ccttatggag attactcaac cggcacaggg tctcagatcc ttttccggca gcagctgggt
     7741 ggtgcaagaa ccaataaccc cctctggctt ttcagccagc caggtacgcg gaagacctct
     7801 gttgtttgcg gagagaacct atggaaatgg cgtttgcatg attacctgga gaacggcgat
     7861 catgcgcagt tcgatgaact tataggcaag accattcaat acctggcaac caaagaagaa
     7921 gaacagcagt tccgggtaag tgcaagcaga aatgtgttca gagaagttga gcctgtaaga
     7981 ctggatgcag aattgtataa tgatgcctat gaacttatca atgaacctga tgtagcctgt
     8041 gtgatcaggg gggaagatgg taatgaatat cctttcacct tcagtcgctc cggtcagcgc
     8101 tataccctgg atgcaggata tttgcctccc ggcagatata ccgtgcaggg aactaccaga
     8161 tacaacgaca aaccactcca ggacgaagct gctttcagtg tagtggcggt acaactggaa
     8221 acttccaata cggtggctga tcatcagttg ctgtacaaac tggcaagtga tcacggcgga
     8281 caaatgttcc gtcccgatca gctgaatgca ctggcagatt cactgttgca aagtgcctgg
     8341 gcgaaaccga cattgcgtga aaaaatacag acacgttcgg tcatcaatct gcgctggctt
     8401 ctgttcctca tcatcggttt gctggccgta gagtggtttg taagaaagtg gagcgggggt
     8461 atttaggagt gagagggagg agtgagagtg agagtgagag ggaggagtga gagtgagagt
     8521 gagagggagg agtgaggagt gaggagtgag taatttgggg aagcaaaata gaagaactgc
     8581 aggatcgcat ccgg
//
LOCUS       DAOMAX010000101         8580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1189_length_8580_cov_4.227566, whole genome shotgun sequence.
ACCESSION   DAOMAX010000101 DAOMAX010000000
VERSION     DAOMAX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_8580_cov_4.227566"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2177
                     /locus_tag="PK742_06715"
     CDS             <1..2177
                     /locus_tag="PK742_06715"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQU76318.1"
                     /translation="QEKEKQLILKEYRNLLRSLKETITTSERKQLRLAFEMALEAHKD
                     MRRKSGEPYIIHPLQVAQIVVKEMGLNATAAICAILHDVVEDTELTLDDIKREFGGKV
                     AKIIDGLTKVSGVFDLTSSIQAENFKKLLLTLADDVRVILIKIADRLHNMRTMDSMPR
                     KKQLKIASETLFLYAPLAHRLGFYAIKSELEDLGMKYTESETYKEIARKLSDTKRERS
                     RYINEFTRPIKERLDEEGMKYQIYGRPKSIHSIWNKMRTKHVTFEEVYDLFAIRIIID
                     SPQNKEKADCWKVYSIVTELFTPNPDRLRDWISTPKANGYESLHTTVMGHRGRWVEVQ
                     IRTVRMDEIAEKGFAAHWKYKEGTADKSLDTWLREIRELLRNPDYNALEFLDDFKSNL
                     FSKEIYVFTPKGDLKILPQDSTALDFAFEIHTDVGYHCIGAKVNHKLVPLSHRLSNGD
                     QVEVITSRKQKPNEDWLGFLITSRARSKVKHALKEEKRQVALVGRDMLEKKMKSLKLP
                     LDDKNMAELMSYYHQPTSLELFYRIQNKDIDLKDLKNFQIKGGRIIAPKRQDKGKGTI
                     NPNLITANADLLIFDEMSDKIAYSLAKCCNPIVGDDVFGFITVNEGIKIHRTNCPNAT
                     ELLSKYGYRAVKTRWTKQQEIAFLTGLKITGVDDVGLVNKVTNVISGEMKLNMQSMTI
                     GAKEGIFEGTFMVYVKNTDELEDLIHRISSLKEITSVTRIET"
     gene            2174..2677
                     /locus_tag="PK742_06720"
     CDS             2174..2677
                     /locus_tag="PK742_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQU76319.1"
                     /translation="MSKQAEGKDKPGLIDEVKNIFAAHMEQHGLRKTPERFAILEEIY
                     RRNDHFDAETLYNEMLRQQYRVSRATVYNTLELLLTCDLVSRHQFGKNQAQYEKSYGY
                     KQHDHIICVDCKKVVEFCDPRIQQIQSMMGELLNFRITHHSLQMYGICGTCQKARELA
                     AEKATKT"
     gene            2680..3039
                     /locus_tag="PK742_06725"
     CDS             2680..3039
                     /locus_tag="PK742_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQU76320.1"
                     /translation="MEYQVERKENSIALISVRGRIVGEYQLVPLTEEVDELLEDQYRH
                     IIFDLSQLEFMTSAGLSFFLKTLTRVRHLDGEVILVALNHLLNDLLVTTKLNAVFIIE
                     PDEEAAMHYLKETGAVH"
     gene            3115..4383
                     /locus_tag="PK742_06730"
     CDS             3115..4383
                     /locus_tag="PK742_06730"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQU76321.1"
                     /translation="MKVDVLLGLQWGDEGKGKIVDYLAEKYDVVARFQGGPNAGHTLI
                     LNNGQKFVLHTIPSGIFLPHTRNLVGAGVVIDPVTFGMEIARLRETGVDPAGRLFISR
                     KAHLILPTHKMLDAASEASKGHDKIGSTLKGIGPAYMDKTGRNGLRVGDLELSDFRER
                     YEKLKAKHLELLALYGDKPTEIAGEKEWLAAVEELLKLDIVDGEYFIHDALTSGQKVL
                     AEGAQGSMLDVDHGTYPFVTSSNTITAGVCTGLGIAPHMVGEVIGISKVYCTRVGSGP
                     FPTELFDEEGDELRRIGNEFGATTGRPRRCGWIDLPALKYTIMLNGVTQLALTKVDVL
                     NTFASVKACVAYKHKGQTTDRVPFDLVGADIEPVYQAFPGWQCELDGEGGRSGLPQTL
                     QDYLQFLETALGVPVTMLSAGPERDKLMEF"
     gene            4386..5681
                     /locus_tag="PK742_06735"
     CDS             4386..5681
                     /locus_tag="PK742_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQU76322.1"
                     /translation="MDRIREVFTCEDPVLFRQQLLQWISAFDVYAVLDSCGNDVYGPP
                     AFDMLCGAGMASAISLEAGDAFQALESFAATGDWLFGHLNYDLKNETESLSSSHPDSI
                     GFPDLFFFRPTILIRLQGHEISIESLTASPKQIMDTIVCLEPLSSYQPGAMQMHPVMP
                     RSAYLNKVEAIRRHIINGDVYELNFCQEFLGKNARLEPVKVFDALCAADKAPMSVLHR
                     NGDRYLISASPERFLMKQGNRLLSMPIKGTIRRGAGADDEELKEQLRQSIKDRAENVM
                     ITDLVRNDLTRFAKTGSIRVDELFGVYPFARVFQMISTISAELQDDAVPVKTIRQAFP
                     MGSMTGAPKVKAMELIDRYEVSRRGLYSGAFGYFTPDADFDFNVVIRSVLYNQRSGVV
                     SVQAGGAIVYDSDPVQEYEECLLKASSLFDVLRAELIQQ"
     gene            complement(5668..6948)
                     /gene="tyrS"
                     /locus_tag="PK742_06740"
     CDS             complement(5668..6948)
                     /gene="tyrS"
                     /locus_tag="PK742_06740"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQU76323.1"
                     /translation="MSNFVEELRWRGMLADIMPGTEDHLNKGMTTGYIGFDPTADSLG
                     VGNMVQVMTLLHFQRCGHKPIALVGGATGMVGDPSGKSAERNLLDEAALRHNVECQQK
                     QLEKFLDFDCGANSAEVVNNYDWMKDFSFLGFLREVGKHITISYMMAKDSVQTRLEKG
                     ISFTEFSYQLLQGYDFYYLWKNRGVMLQMGGSDQWGNITTGNELVRRMEGGEVFAVCT
                     NLIKKADGTKFGKTEGGNIWLDPKRTSPYKFYQFWLNASDTDAETYIKIFSLKDRTTL
                     ESLISEHAAEPHLRKLQKALAEEVTARVHSEEDLEKAIAASDILFGKSTSESLRKLDN
                     DTFLDVFEGVPQFHIARSVLESGADLPSLLCEHSNVLPSKGELRRALQEKSLQLNKEK
                     IEDADMTVTTADLIGNRYLLFQRGKKNYYLFIAE"
     gene            7047..7721
                     /locus_tag="PK742_06745"
     CDS             7047..7721
                     /locus_tag="PK742_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76324.1"
                     /translation="MNFLSHYYHELPVSDPYFVAGVIMPDILSNYSKRNGEKVRLNPS
                     KLKAGVEAPYRSLADGVRQHYFVDGFFHDSGFFDEMTSVIEEKVHALDFQCFRRRLFA
                     FSHVFLELMLDRSLLLEDRTIADNMYGLLEELDAKTVSGFFNLQEQEDHADGIARHIQ
                     VFTERRFIYHYADEERLMMILDAINSGFGNPAFTQSDRMQLTHLIHDMDGILRTEKFP
                     KFPADS"
     gene            7723..>8580
                     /locus_tag="PK742_06750"
     CDS             7723..>8580
                     /locus_tag="PK742_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76325.1"
                     /translation="MLKRHLLIILLMCGVFSAQAQAVRKYSNEFLSIGISGQGMAMGG
                     SMVAGVDDVTAGYWNPAGLLLIGNDMQVGLMHAEYFAGIAKYDYGSVAIPLQQKDAVL
                     AFSLIRFGVDDIPNTLFLIGPDGSINYDNITSFSVADYAFLSSYATRLAWNDLRIGGS
                     AKIIHRTAGYFATSWGFGLDIGAQMEKGNWEFGVMGRDITGTFNAWSFGFTEEDQAVL
                     LATGNALPESSLEITTPRIILGAGYNMALGEHFTLRPELNLDLTTDGKRNVLLAADPV
                     SMDPHLGIEA"
BASE COUNT         2216 a         2086 c         2259 g         2019 t
ORIGIN      
        1 accaggaaaa agagaagcag ctcatcctga aagagtaccg caatctactg cgttcgctga
       61 aggagacgat caccaccagt gaacggaaac agctgcgtct tgcctttgaa atggcactgg
      121 aggcgcataa agatatgcgc agaaaatctg gtgagcccta catcattcac ccgcttcagg
      181 tggcacagat cgtggtcaag gaaatgggtc tcaatgccac tgccgctatc tgtgccattt
      241 tgcatgatgt ggtggaagat actgagctta ccctggatga tatcaaaagg gagttcgggg
      301 ggaaggtggc gaagatcatt gacggactga cgaaagtatc cggagtgttc gatctgacct
      361 cctccattca ggcagaaaat ttcaagaaac tgttgctcac cctggccgat gatgtcaggg
      421 tcatcctgat caagatcgcc gacaggctgc acaacatgcg tacgatggac agtatgcccc
      481 ggaaaaagca gctgaagatc gcctctgaaa ccctgtttct atatgcacca ctggcacaca
      541 ggctggggtt ttatgccatc aagtctgagc tggaagacct gggcatgaaa tacacagagt
      601 cagagactta caaggagatc gcccgtaagt tgagtgacac caaacgggaa cgcagcaggt
      661 atatcaatga atttacacga cccatcaaag agcgtctcga tgaagagggt atgaaatacc
      721 agatctatgg aaggcctaaa tccatccatt ctatctggaa caagatgcgc accaagcatg
      781 tgaccttcga agaagtatat gacctcttcg ccattcggat catcatcgat tcaccacaaa
      841 ataaggaaaa ggcagattgc tggaaggtgt attccatcgt tactgaatta ttcaccccca
      901 atcctgaccg tctccgcgac tggatcagca cgccgaaggc taacggttat gagtcgctgc
      961 ataccacggt catggggcat cgcggtcgct gggtggaagt gcagatacgc actgttcgca
     1021 tggatgagat cgcagagaag ggttttgcag cgcactggaa atacaaggaa ggaacagcag
     1081 ataaatcact tgatacctgg ctgcgtgaga tccgtgaact gttgcggaat cccgactata
     1141 acgccctgga atttctggac gattttaaga gcaatctgtt ctcgaaagag atctatgtat
     1201 ttacacccaa gggtgacctc aagatcctgc cacaggacag tacggcactg gactttgcct
     1261 ttgagatcca caccgatgtg ggctatcatt gtattggtgc caaggtcaac cacaagctgg
     1321 tacccctgag ccaccgcctc tccaacggag accaggtgga ggtgatcacg agtcgtaagc
     1381 agaaacccaa tgaagactgg ctcggatttc tgatcaccag ccgggcgagg tccaaggtca
     1441 agcatgcact gaaagaagaa aagcggcagg tggcactggt gggaagagac atgctggaaa
     1501 aaaagatgaa gtcactgaag ctgcctctgg acgataagaa catggcagaa ttgatgtctt
     1561 actatcacca gcctacctcg ctggaattgt tctatcggat acagaacaaa gacatcgacc
     1621 tgaaggacct gaagaatttt cagatcaaag gaggccgtat catcgcaccc aagcggcagg
     1681 ataaagggaa gggaaccatc aatcccaacc tcattaccgc caacgcagac ctgctcattt
     1741 ttgatgagat gagcgacaag atcgcctact cattagccaa gtgctgcaat cctattgtgg
     1801 gcgatgatgt gttcgggttc atcacggtca atgaaggcat caagatccat cgcaccaact
     1861 gccccaacgc tacggaactg ctcagtaaat atggttaccg ggcggtcaag accaggtgga
     1921 ccaaacaaca ggagatcgca ttcctcacag ggcttaagat caccggagtg gacgatgtgg
     1981 ggcttgtcaa taaagtcacc aatgtgatca gcggcgagat gaaactcaac atgcaaagca
     2041 tgaccatcgg cgccaaagaa ggtatctttg agggcacctt catggtgtat gtcaagaata
     2101 ccgacgagct ggaagacctg atccatcgca tcagcagcct gaaggagatc accagtgtaa
     2161 cgaggatcga aacatgagca agcaagcaga aggcaaagac aaacccggac tgatagacga
     2221 agtgaaaaac atctttgctg cccatatgga acagcatgga cttcgcaaaa cccccgaacg
     2281 cttcgccatc ctcgaggaga tctaccggcg taacgatcat tttgatgctg agacgctgta
     2341 caatgaaatg cttcggcagc aataccgggt aagccgggca acggtctata ataccctgga
     2401 actgctgctt acctgcgatc tggtgtcgcg tcaccagttt ggcaagaacc aggcacagta
     2461 tgaaaaatca tacggataca agcaacatga tcatatcatc tgtgtggatt gtaaaaaagt
     2521 tgtggaattt tgtgatccac gcatccagca gatacagagt atgatgggcg aattgctgaa
     2581 ctttcgcatc acccaccact ctttgcagat gtatggcatt tgcggtacct gccagaaagc
     2641 ccgagagctg gcggcagaaa aagcgacgaa aacgtgagca tggaatacca ggttgaaaga
     2701 aaagagaaca gcattgcact gatcagcgtc aggggacgga tcgtgggcga gtaccagctg
     2761 gtacccctta ccgaagaagt ggatgaactg ctggaagacc agtaccgcca tatcatcttt
     2821 gacctgtcgc aactggagtt catgacatct gccggattgt ctttctttct aaagaccctg
     2881 acccgggtgc ggcacctgga cggcgaggtc attctggttg cactcaacca tttgttgaat
     2941 gatctgctgg taacaacaaa actcaacgct gtttttatta tcgaacctga tgaggaagca
     3001 gctatgcatt acctgaaaga aaccggggcg gttcactagc cgccgggccc gtgttcatat
     3061 tttctgactg aattgtcggc tccattcctg aattttgcct ttcaagcgac acacatgaaa
     3121 gtggatgttt tattagggct gcaatggggc gacgaaggaa aaggcaagat agtggactat
     3181 ctggccgaaa agtatgatgt agtagcccgt tttcagggcg gcccgaacgc aggccatacc
     3241 ctgatcctga acaacgggca aaaattcgtg ctgcatacca tcccatcagg gattttcctg
     3301 ccgcatacca gaaacctggt gggagccggt gtggtcatcg atcccgtaac cttcggcatg
     3361 gagatagcac gtttgcggga aaccggtgtg gatcctgccg gcaggctgtt catctcccgc
     3421 aaagcccacc tcatcctgcc tacccataaa atgctggacg ctgcttcaga agcatccaaa
     3481 ggacatgaca agatcggtag tacgctgaaa ggaataggtc cggcttatat ggacaaaaca
     3541 ggacgaaacg gactgcgtgt gggcgatctg gagctctccg attttcgtga gcgatacgaa
     3601 aaactcaaag ccaagcacct ggagttgctg gcactgtatg gtgacaagcc cacagagatt
     3661 gcaggagaaa aagaatggct tgccgcagtg gaagaattac tgaagctgga catagtggac
     3721 ggtgaatatt ttattcatga tgctttgacc agcggacaaa aagtactggc cgaaggtgca
     3781 cagggcagca tgctggatgt ggaccacggc acctacccgt tcgtgaccag cagtaacacc
     3841 atcacagccg gagtctgcac tggtctgggc attgctccac acatggtagg cgaggtgatc
     3901 ggaatttcca aagtgtactg taccagggtg ggtagcggac ctttccctac tgaactgttc
     3961 gatgaagaag gcgatgaact tcgtcgtata ggaaatgaat tcggcgccac tactggcaga
     4021 ccccgcaggt gcggatggat agacctgccg gctctgaaat ataccatcat gctgaacggt
     4081 gtcacccagc tggcactgac caaagtggat gtgctcaaca cctttgcctc agtgaaggct
     4141 tgtgtagcct ataagcataa agggcaaacc acagacaggg tacctttcga tctggtaggt
     4201 gccgatatcg aaccggtgta tcaggcattt ccgggctggc agtgtgagct ggatggagag
     4261 ggcggaagaa gcggattgcc gcaaaccctt caggattatc tgcagttcct cgaaacagcc
     4321 ctcggagtgc ccgttaccat gttatctgca ggtcctgaaa gggataagct catggaattt
     4381 taataatgga ccgcatccgg gaagtcttta cctgtgaaga cccggttctg ttccggcagc
     4441 aactgctgca gtggatctcg gcgttcgatg tatatgccgt actggacagc tgtggcaatg
     4501 atgtatatgg cccgcccgca tttgatatgc tttgcggagc aggtatggca tcagcgatct
     4561 ctctggaagc aggcgatgct tttcaggcac tggaatcatt tgcagcaacc ggcgactggt
     4621 tgttcgggca tctgaactat gacctgaaga atgaaaccga atctctgagc agcagccatc
     4681 ctgattccat tggtttcccg gatctttttt tctttcgtcc gacgatcctc atcagactgc
     4741 agggccatga aatcagtata gaaagtctga cggcctctcc aaagcagatc atggatacca
     4801 tagtttgcct ggaaccactt tcatcttatc agcccggtgc catgcaaatg catccggtca
     4861 tgccccggtc agcgtatctc aataaggtag aggcgatccg ccggcacatc ataaacggcg
     4921 atgtgtatga actgaatttc tgccaggaat ttctgggaaa aaatgcccgt ctggaacctg
     4981 tcaaagtatt cgatgccttg tgcgctgcag ataaagctcc catgagtgtg ctgcaccgga
     5041 acggcgacag gtatctcatc agtgcaagtc cggaacgctt tctgatgaag cagggtaaca
     5101 ggctgctttc catgcccatt aagggtacca tcagaagggg agcaggtgca gatgatgaag
     5161 agctcaaaga gcagctgcga caaagtatca aagaccgggc agagaatgtc atgatcactg
     5221 atctggtgcg caatgatctg accaggtttg ccaaaacggg cagcatccgg gtagatgagc
     5281 tgtttggggt ctatcctttc gcacgggtat ttcagatgat cagtacgatc agtgcagagc
     5341 tgcaggatga tgccgttccc gtgaagacca tacgccaggc atttcccatg ggaagtatga
     5401 ccggagcccc gaaggtcaaa gcgatggaac tgattgacag gtatgaggtc agcaggaggg
     5461 gattatactc aggagccttc gggtacttca cacccgatgc cgactttgac ttcaacgtgg
     5521 tgatcagaag tgtgctctat aaccagcgat caggggtggt atcagtgcag gccggcgggg
     5581 ctatagttta tgattctgat ccggtgcagg agtatgaaga atgtctgctg aaagcctcct
     5641 cgctctttga tgtgttgcgg gctgaactta ttcagcaatg aacagatagt agttcttctt
     5701 gcctctctgg aacagcagat atcggttgcc gataagatct gcagtggtga ccgtcatatc
     5761 cgcatcttcg atcttctctt tgttgagctg cagggatttt tcctgcaggg ccctgcgcaa
     5821 ttctcccttg ctgggaagca cattgctgtg ttcacacaag agggagggga ggtcagcacc
     5881 tgattccagc accgatctgg caatatgaaa ttgaggcacc ccttcgaata catccaggaa
     5941 ggtatcgttg tccagtttgc gcaaagactc gctggtggac tttccaaaga ggatgtcgct
     6001 ggcggcgatt gctttttcca gatcttcctc agagtgcacc ctggcggtca cctcttctgc
     6061 cagtgccttt tgcagtttgc gcaggtgcgg ttctgctgca tgttccgaga tcagagattc
     6121 cagggtggtc cggtctttca gtgaaaatat tttaatataa gtttcggcat cagtatcaga
     6181 ggcattgagc cagaactggt agaatttata aggactggtg cgtttcggat cgagccagat
     6241 gttgccacct tcggtcttgc cgaatttagt accatcagcc ttcttgatca ggttggtaca
     6301 aacggcaaat acttcgccgc cttccatcct ccgcaccagt tcattaccgg tggtaatgtt
     6361 tccccactga tcacttcctc ccatctgcag catcacaccc ctgttcttcc agaggtagta
     6421 gaagtcatat ccctgcaata gctggtaaga gaactctgta aaggagatgc ctttttccag
     6481 acgtgtctgt acgctgtctt tcgccatcat atagctgatg gtgatgtgct ttcccacttc
     6541 tctcagaaaa ccaagaaagg agaaatcttt catccagtcg tagttgttca ctacctcagc
     6601 gctgttagca ccgcaatcga aatccagaaa tttctccagt tgtttttgct ggcattccac
     6661 attgtgccgg agggccgctt catccagcag gttgcgttct gcactttttc cactggggtc
     6721 gcccaccata ccagtggcac cacctaccag tgcaatgggt ttgtggccac agcgctgaaa
     6781 atggaggagg gtcatgacct gaaccatgtt gcccacgccc aggctatcgg cggtagggtc
     6841 aaaaccgata taaccggtgg tcattccctt gttgaggtga tcttccgtgc ctggcatgat
     6901 atcagccagc attccccgcc agcgcagctc ttccacaaaa ttcgacatgc cgcaaaaata
     6961 ggccattcac gtcattgcgc tgtcaaagct gacatggttt tcagccgtta attgaacaat
     7021 tttgacggaa acgcacttga accagtatga acttcctgag ccactactac catgaattgc
     7081 ctgtcagcga tccgtatttc gtggccggtg tgatcatgcc tgacatactt tccaactact
     7141 ccaaacggaa cggcgaaaag gtgcgcctga atccatcaaa actgaaagcg ggagtggaag
     7201 cgccttaccg gtcgctggca gatggggtca ggcagcatta ttttgtagat ggcttttttc
     7261 atgactccgg tttctttgac gaaatgacca gtgtcattga agaaaaggtg catgcccttg
     7321 attttcagtg cttccggagg cgattatttg cctttagcca cgtctttctg gagctcatgc
     7381 tggaccggtc actgctgctg gaagaccgaa ccattgctga caatatgtat ggcttgttgg
     7441 aagagctgga cgccaagacg gtaagtggtt tctttaacct ccaggagcag gaagaccatg
     7501 ccgatgggat agccaggcat attcaggtgt tcacggaacg ccgtttcata taccattatg
     7561 ccgatgaaga acgtttgatg atgatcctgg atgccattaa cagcggtttt ggtaaccctg
     7621 cgttcactca atcagacaga atgcagctga cccacctgat tcatgacatg gacgggatat
     7681 tgcgcactga aaagtttcct aaatttccgg ctgattcctg atatgctgaa acgccactta
     7741 ttaatcatac tgctgatgtg cggagtgttc tctgcacagg ctcaggctgt tcgaaagtat
     7801 tccaatgagt ttttgtcgat cggcatttcc ggccagggta tggctatggg aggcagcatg
     7861 gttgccgggg tggatgatgt gactgccgga tactggaatc cggccggctt actgctcata
     7921 ggcaacgata tgcaggtggg gctgatgcac gctgagtatt ttgcgggcat cgccaagtat
     7981 gactatggaa gtgtggccat tccgctgcag cagaaagatg ccgtactggc attttcactg
     8041 atccgttttg gtgtggatga tattcccaat acgctctttc tgattggacc tgacggaagc
     8101 atcaactacg ataacatcac ttctttttca gtagctgatt atgcatttct cagttcctac
     8161 gccaccaggc tggcatggaa tgacctgcgc ataggaggaa gtgccaagat cattcaccgt
     8221 actgccggat actttgccac ttcctggggt ttcggattgg acatcggggc acagatggag
     8281 aaaggtaact gggaatttgg tgtcatgggc cgcgacataa ccggaacctt caatgcctgg
     8341 tcattcggtt tcacggaaga agatcaggct gtgttgctgg caaccggaaa tgcgctacct
     8401 gaaagctcgc ttgagatcac cacgccccgc atcattctgg gtgccggtta caacatggca
     8461 ctgggcgaac attttaccct gaggcctgaa ctgaatctgg accttaccac cgatggtaag
     8521 cgcaatgtct tgctggcagc agatccggtc agcatggatc cgcatctggg cattgaagcc
//
LOCUS       DAOMAX010000102         8472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1225_length_8472_cov_4.394677, whole genome shotgun sequence.
ACCESSION   DAOMAX010000102 DAOMAX010000000
VERSION     DAOMAX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1225_length_8472_cov_4.394677"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK742_06755"
     CDS             complement(<1..463)
                     /locus_tag="PK742_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQU76326.1"
                     /translation="MKVVLLGTAHPFRGGLASFNERLILEFIRQGHEASISTFTTQYP
                     GILFPGTSQFSKSPAPDIPIKREVSSIDPVSWWKLGRKLKKQSPDILIIKYWLPFMAP
                     CFGTVAAIARKNKHTKVICIADNIIPHEPKFYDRFFTRYFLKRCDAFITMSN"
     gene            complement(460..1410)
                     /locus_tag="PK742_06760"
     CDS             complement(460..1410)
                     /locus_tag="PK742_06760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU76327.1"
                     /translation="MQVSVVIPLFNEEESLPELAEWLGHVLEGKDWEVIFVDDGSKDG
                     SWRVVEQLHRQETRFKGIRFRRNYGKSAALSQGFGMAQGDVVITMDADLQDSPDEIPE
                     LMDMISKDGFDLVSGWKKKRYDPVMKTIPSKFFNGVTRMMSGIRLHDFNCGLKAYRRE
                     VVKSIEVYGEMHRYIPVIAKAAGFNRIGEKVVEHRARKYGVTKFGWERFVNGFLDLLS
                     IMFVSRFGKRPMHLFGTVGTLSFLTGGVLTIWLIAVKLIRLHNGVPFRQVTDQPLFYV
                     ALVAMIVGVQLFVAGFLGELISRSSIDRNKYQVDQILDRP"
     gene            complement(1412..1957)
                     /locus_tag="PK742_06765"
     CDS             complement(1412..1957)
                     /locus_tag="PK742_06765"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQU76328.1"
                     /translation="MENLDQNQLTEEQGVPRNSIVWKFAIIFGLIFIIYIILSNMFRE
                     QMMEQSTLVTILTGLIMLAGIIITQMQVRDKSFMGTMNFSKGLGTGMLFTIYLTLIIV
                     VFGLLFNTVIFPETLQEQQQLAIDQMREQEMSQEQIDQAMKFTGFMFKPVGAALVGLF
                     SNLFFGLILSLIGAAVTSRNK"
     gene            complement(1960..3231)
                     /locus_tag="PK742_06770"
     CDS             complement(1960..3231)
                     /locus_tag="PK742_06770"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQU76329.1"
                     /translation="MQLLIQRIRIVDPASPHHFSVRDIRISNGIITAISEKLHAEEGE
                     TLWQADGSSVSPGWVDLLAASGDPGQEYREDLHSLAAAAVAGGYTGVAVMPSTHPALQ
                     TKADIEYVIHKTAGKAVECYPLGAVTRNRDGKELTEIYDMRSAGAVAFTDADLPVADA
                     GIMLRTLQYVKQFDGTIINVPSDHRIVGNATINEGVMSTRLGMYGIPDVLEEVMVERD
                     ILLAEYAASKVHITGISTARALDRIRLAKQKGIQVTCSVTPYHLYFDEEMLGDYDTNF
                     KVNPPLRTARDVAALKEGVADGTIDAVASFHIPWDTDAKACEFEYAGFGMATLETCFG
                     AVCSALTGYVPVDKVIQLFTSGPRNILGLPPVSIAEGQTANLTLFNETDAWTRSDFVS
                     KGVNQAFAGQPLTGRPLATFFANQFVTLINN"
     gene            complement(3242..5257)
                     /locus_tag="PK742_06775"
     CDS             complement(3242..5257)
                     /locus_tag="PK742_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HQU76330.1"
                     /translation="MSFLAPGMLWGLLLLAVPVIIHLFSFRRFKTVYFPDIRFLKQVR
                     EESATRNRLKQWLILAARMLAVFFLVMAFAQPFLPGAEKAVTGSSHAVSVYVDNSFSM
                     EAEHDGLSLLEAARQQARAVVNAYGLDDRFQILSNTPDALSQQWLSKEEALDVIGLIE
                     TGPEVQTISEVTVQQFNMLRRAGDAEPAAFLISDFQRSTTDAISDTSYAVTCIHLGSR
                     SVANLAVDSAWWESPVQVNGEQSTLLYQVTNYGDEDRKNMGVTLRIDGQVKGVETIDV
                     RGGETIRDSISFLVQGTGWQELELSVEDYPVNFDDRLYLSFRPVTRVRVTEIGFGSTD
                     RILPGIFSNALFSFASVPAGNIDYNRLADNDLIILHELDDLSSGLQSSLEKALRAGTS
                     LLLIPGSTMNLQAVNSFLAPMGAVALEPFSGKRQASSVNLQHAIFDGVFDRVPKNLAL
                     PFQQNGWAIKPSVRSDGESVISFADGTPMVYSMPAGSGTLYLLGTPLDQAHTDLPGQG
                     AIFVPMIYRMALLSKGYQIPYLIAGREQWVSLAANGSAAEETVATVSGNGEEFIAAVR
                     QLQDHTEISLYPYAREAGFYKVSADSVSKVIALNADRRESQLECLSVNELKELGNDRL
                     AVLEGSERFVADSVLRLRDGRQLWKICVILALLALASEILLLRFLPV"
     gene            5396..5812
                     /locus_tag="PK742_06780"
     CDS             5396..5812
                     /locus_tag="PK742_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76331.1"
                     /translation="MKRYLTCVMLLFWLIPVQSQIPDNQIGSDQEVFSQDPVDFDMFM
                     TDELGNEEAPLIVQMYPVPVQDYLVVRLSCRYTLHNVRITVLDRQGYQVFGMRGEEMG
                     PGTKRVVIPFEELPAGNYYIWIQYEEGMVTRQVIRQ"
     gene            complement(5814..6638)
                     /locus_tag="PK742_06785"
     CDS             complement(5814..6638)
                     /locus_tag="PK742_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU76332.1"
                     /translation="MSNQLLKGKRGIIFGALDESSIAWKVAEKCFEEGATFTLTNAPI
                     AMRMGEINKLAEKCNTTIIPADATNMEDLEKLIDESVERLGGKLDFILHSIGMSPNVR
                     KNRPYTDLSYDFLQKTLDVSAISFHKVMQTAYQKDAINEWGSVVGLTYIAAQRTFPSY
                     SDMAEAKSTLESIARSFGYHYGKHRKVRVNTISQSPTWTTAGSGVKGFDAFYAYADKL
                     SPLGNASAESCADYCLFLFSDMSRMVTMQNLYNDGGFSSVGVSDEIMQMLTKELEQ"
     gene            complement(6647..7177)
                     /locus_tag="PK742_06790"
     CDS             complement(6647..7177)
                     /locus_tag="PK742_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76333.1"
                     /translation="MRNTILCTLILLIAFPVFGQQASRQKKAKPYRVEINDASFTNSK
                     PEFGATLSGIYEHDTLMEMKAWFGFSYGDIQRTFFYWKGELITVTEVHRVYNAYDPGM
                     PVDSISPSFNARYVYENGKLTLIKQNGTYSLIDAPQDKASVEAMYLNFSEQYTRALDE
                     KRAVKKNRKKIKKRNQ"
     gene            7226..7831
                     /locus_tag="PK742_06795"
     CDS             7226..7831
                     /locus_tag="PK742_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQU76334.1"
                     /translation="MNTGRDTSRSRLFREPVWLMIHLVLLGIIILFAGLTAAYLIGPD
                     AGEEAGFRIPRIFWLSSILVLLVSLSMRRVLKAYERDNSSTLSGWLLAATVTALLFVV
                     CQWIGWTGLHQQGITLQDTPAAGYLYVLSGLHVVHVGVGIILLLVSLTKSRRLLQHAA
                     TALVYFSDPVRRRRLELLARYWHTIDYLWLFLFLVFLYKHA"
     gene            complement(7868..8257)
                     /locus_tag="PK742_06800"
     CDS             complement(7868..8257)
                     /locus_tag="PK742_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76335.1"
                     /translation="MKKLTMLITAGLMFLGASAYAQNAGQVTKQQKKDHMTEKYSESG
                     RSKAEVKQHHAASADGNDRQEKKEAMIDHYGSKDAAKEAAREHRMEQREGADRKDQMR
                     ARANEDPARKDAMQQKMQEKKRKKNGQ"
     gene            8379..8452
                     /locus_tag="PK742_06805"
     tRNA            8379..8452
                     /locus_tag="PK742_06805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8413..8415,aa:Pro,seq:ggg)
BASE COUNT         2085 a         2115 c         1973 g         2299 t
ORIGIN      
        1 tattggacat ggtgatgaag gcatcgcagc gtttcagaaa atagcgggtg aagaatctgt
       61 catagaactt cggttcatgg gggatgatat tatctgcaat gcagatcact ttagtatgct
      121 tattctttcg tgcgatagca gccaccgtac caaagcatgg tgccataaaa ggcaaccagt
      181 acttgatgat caggatgtca ggactctgct ttttgagttt tcttcccagt ttccaccagc
      241 tgaccggatc aatgctggaa acttcccgct taataggaat atcgggggcc ggcgacttgc
      301 tgaactggct ggttccggga aagagaatgc cgggatattg ggtggtaaaa gtgctgatgg
      361 aagcttcgtg gccctgcctg atgaattcca gtatcagcct ttcattgaag gaagccaggc
      421 ctccgcgaaa aggatgcgct gttcccagca ggaccacctt catggacgat ccaatatttg
      481 atcaacctgg tatttgttgc gatctataga agatcttgag atcagctcac ccagaaagcc
      541 tgcaacaaat aattgcacgc caacgatcat agccaccagt gctacataga acaggggttg
      601 atctgttacc tgccggaagg gtacaccatt atgcaatctg atcagcttaa cagcgatgag
      661 ccagatggtc agtacaccac ctgtcaaaaa tgaaagtgtc cctactgtac caaacagatg
      721 cattggtctt ttaccaaaac gggaaacgaa cataatggat aacagatcga gaaatccatt
      781 cacaaaacgt tcccaaccga attttgttac gccatatttc cgtgcacggt gttccactac
      841 cttctcacct atcctgttaa atccggcagc cttggcaatt accggaatat aacggtgcat
      901 ctctccgtac acttcgatac tcttgaccac ttcgcgtctg taggctttca gcccgcagtt
      961 aaagtcatgc agcctgatac cactcatcat acgggtaaca ccattgaaga atttagatgg
     1021 tatggtcttc atgacagggt catagcgttt tttcttccat ccactgacca gatcaaatcc
     1081 atctttgctg atcatgtcca tcagttcagg tatttcatcc gggctgtcct gaagatcagc
     1141 atccatggta atgaccacat ctccctgcgc cattccgaac ccctgactga gtgcggcact
     1201 cttgccgtaa ttgcgtctga atctgatgcc tttgaacctt gtctcttgtc tgtgcaattg
     1261 ttccaccacc ctccaggaac catctttgct gccatcgtcc acaaaaatga cttcccagtc
     1321 tttaccctcg agcacatgcc ccagccattc tgccaattca ggcagtgact cttcctcatt
     1381 aaagagcggg atgactacac tgacctgcat actatttatt ccggctggta acagcagctc
     1441 ctatcagtga gaggatgagg ccgaagaaca ggttactgaa cagaccaacc agtgcagcac
     1501 ctacgggttt gaacataaat ccggtaaatt tcatggcctg gtctatttgc tcctgcgaca
     1561 tttcctgttc acgcatctgg tctatggcaa gttgctgctg ttcctgcagg gtttcaggaa
     1621 agatgaccgt attgaacaac aggccgaaca ctacaatgat cagagtcagg tatatagtaa
     1681 acagcatacc tgttcccaga cctttggaaa aattcatggt acccataaac gacttgtctc
     1741 ttacctgcat ctgtgtaatg atgatcccgg ccagcatgat cagtccggtc aggatggtca
     1801 ccagggtgct ctgttccatc atctgctctc taaacatatt acttaatata atatatataa
     1861 taaagatcag gccaaaaatg atggcgaact tccagacgat cgaattacgg ggtacgcctt
     1921 gttcttcagt cagttgattc tggtcgaggt tttccatgat cagttgttta ttagtgttac
     1981 gaattgattt gcaaagaagg tagcaagtgg tcgtcctgta agtggttgcc cggcaaaagc
     2041 ctgattgact cctttagata cgaaatctga tctggtccag gcatcggttt cattgaataa
     2101 ggtcaaattg gctgtctgac cttctgcaat gcttacaggc ggcagaccca ggatgtttct
     2161 agggccggag gtaaacagtt gtatgacttt gtcaacaggt acataaccgg tcagtgcaga
     2221 acaaactgcc ccgaagcagg tttcaagtgt agccattcca aaaccggcat attcaaactc
     2281 gcatgctttt gcgtcggtat cccatggaat atggaaactg gccacggcat caatggtacc
     2341 atcagccact ccttctttta aggcagccac atctctggcg gtgcgcaaag gcggattgac
     2401 cttgaaattg gtatcataat cacccagcat ctcttcatca aaatagaggt ggtatggtgt
     2461 gacagaacat gtaacctgta ttcctttttg tttcgccaga cggattcggt ctaatgccct
     2521 ggctgtagaa ataccggtaa tatgaacctt agacgcagca tattcggcca gcaggatatc
     2581 tctttccacc atcacctctt ccagtacgtc aggaatgcca tacatgccca accgggtgct
     2641 catgacccct tcgttgatgg tggcatttcc cacgatccga tgatcgctgg gtacattgat
     2701 gatggtaccg tcaaactgtt tgacgtattg cagcgtgcgc agcatgatgc ctgcatctgc
     2761 aacaggcaga tcagcatcag tgaacgccac ggcaccggct gagcgcatat catagatctc
     2821 tgtcagctct ttcccatctc tgtttcgggt aacggctccc agcggataac attcaaccgc
     2881 tttacctgcg gtcttatgaa taacatattc aatatctgcc ttggtttgca gggcagggtg
     2941 agtggagggc atgacagcga caccggtata acctccggca actgcagcag ctgcgagtga
     3001 gtgcaggtct tcccggtatt cctgtccggg atcgccggag gcagccaaaa gatccaccca
     3061 tcccggagaa acggatgatc catctgcctg ccataatgtt tctccctctt ctgcatggag
     3121 cttttcagat attgctgtaa tgatcccgtt gctgatgcgt atatcacgaa cagaaaaatg
     3181 atgaggggaa gctgggtcaa cgatgcggat acgctgaatt agcagttgca tgcgatgttt
     3241 tttacactgg cagaaaacgc agcagcagta tctctgatgc cagggctaac agcgctaaaa
     3301 taacgcaaat tttccacaac tgtcggccgt cccgcagtcg cagtacagaa tcagcaacga
     3361 aacgctcact gccttccagc accgcaagcc tgtcatttcc cagttctttt agttcgttaa
     3421 cagacaagca ttccagttgg ctttctcttc tgtcggcgtt cagcgcaatg accttgctga
     3481 ccgaatcagc tgacacttta taaaatccgg cttcgcgggc atacgggtat agactgatct
     3541 cggtatgatc ctgcaactgt ctgactgcag cgatgaattc ctctccgtta cctgaaacag
     3601 ttgccacagt ttcttctgca gcacttccat tggcagcaag tgaaacccat tgctctcttc
     3661 cggcaatgag atagggaatc tgatatcctt tgctcagtaa tgccatccgg tagatcatcg
     3721 gaacaaatat ggcgccctga ccgggcaggt ctgtatgcgc ctgatccagc ggtgttccga
     3781 gcaggtacaa agttccggaa cccgccggca tggaatacac catgggggtt ccatctgcaa
     3841 atgaaatgac tgactcaccg tcagaacgta ctgatggttt gatcgcccag ccattttgct
     3901 gaaacggcag ggcaagattc tttggcacac ggtcaaagac cccgtcaaat atggcatgtt
     3961 gcaggttgac ggaagaggcc tgtcgttttc ctgaaaaggg ctccagggct accgcaccca
     4021 ttggcgccag aaaactgttc actgcctgca ggttcatcgt tgacccgggt atcagcaaca
     4081 aggaagttcc ggcacgcagg gctttttcca gactgctttg caaacccgac gacaggtcat
     4141 ccagttcatg cagtatgatc agatcattat cagccagtct gttatagtct atatttcctg
     4201 caggtacaga tgcaaaactg aacagggcat tgctgaagat tccaggcagt attctgtctg
     4261 tactaccaaa tccgatctct gtgacacgca ccctggttac agggcgaaag ctgagataca
     4321 acctgtcatc aaaattgaca ggatagtctt ccacactcag ttccagttcc tgccagcctg
     4381 ttccctgaac caaaaaagaa atgctgtcgc gaatggtttc acctccacgc acgtcaatgg
     4441 tctctactcc ttttacctgc ccatcaatcc gcagggtcac ccccatattc ttcctgtctt
     4501 catcgccgta attggtcacc tgatagagga gggtactttg ttctccgtta acctggaccg
     4561 gcgattccca ccatgcgctg tcaacagcca ggttggcaac agatctgctg ccaagatgaa
     4621 tgcaggtaac tgcataggag gtatcagaga ttgcatcggt tgttgaccgt tgaaaatcgc
     4681 tgatcaaaaa tgcggcaggt tcagcatctc cggcccgtct cagcatattg aattgctgca
     4741 ccgtcacctc tgaaatggtc tgtacttccg gaccggtttc gatcagccct attacatcca
     4801 gggcctcttc tttagaaagc cattgctgag acagggcatc tggtgtatta ctcaaaatct
     4861 gaaaacgatc atcaagacca tatgcattta ccacggcccg ggcctgctgc cgggctgctt
     4921 ccagtaatga gagcccatca tgctccgctt ccatactaaa ggaattgtcc acatagacac
     4981 tcacagcatg tgagctgccg gttaccgcct tttctgctcc gggcagaaaa ggctgggcaa
     5041 atgccatgac aagaaagaaa acggccagca ttctggcagc cagtatcaac cattgcttca
     5101 gtctgtttct ggttgcactt tcttctctga cctgcttcag aaatctaatg tcaggaaagt
     5161 aaaccgtttt aaaacgacga aagctgaaca ggtggatgat taccggcacg gccagcaaca
     5221 gcaaacccca caacatacca ggtgccagaa aactcatcat gtaaagatag tatccaccac
     5281 ctaacacagc acagttcaga atattgttta ctccggccga acatcaggtc tttacatgac
     5341 attcatcaat tgctgacagg ccataccatc ctaacttgga aacaaaggca aagccatgaa
     5401 aagatatctt acctgcgtga tgcttctgtt ctggttgata ccggtacaaa gccagattcc
     5461 tgataaccag atcggcagcg atcaggaggt attttctcag gatcctgttg attttgatat
     5521 gttcatgacc gatgaacttg gaaacgaaga ggccccgttg atcgtgcaga tgtatccggt
     5581 tccggtgcag gactatctgg tggtgcggct cagttgcagg tacactttgc ataacgtgcg
     5641 catcactgtg ctggacaggc aggggtatca ggtatttggc atgcgtggtg aagaaatggg
     5701 ccccggcacc aaacgggtgg tcataccctt tgaagaattg cctgcaggca actattacat
     5761 ctggatacaa tacgaagaag gtatggttac ccgccaggtg atcaggcaat gacctattgc
     5821 tccagttctt tggtgagcat ctgcatgatc tcatcgctta caccaacgct gctgaatccc
     5881 ccgtcattgt agaggttttg catcgtgacc atccggctca tatcgctgaa caggaagagg
     5941 cagtagtcag cacagctttc tgcacttgca tttccaagcg gagagagttt atctgcatag
     6001 gcataaaagg catcaaatcc tttgaccccg ctgcctgctg tggtccaggt gggcgactga
     6061 gaaatcgtat tcacacgcac tttgcggtgc ttaccatagt ggtaaccgaa actgcgggca
     6121 atactttcca gagttgattt cgcttctgcc atatcagaat aagagggaaa agtgcgttgg
     6181 gcagctatat aggtcagccc cactacacta ccccattcat ttatagcgtc cttctggtag
     6241 gctgtctgca tgaccttatg gaaagagatg gcagaaacgt ccagtgtttt ttgaagaaaa
     6301 tcatatgaca gatctgtata aggtctgttc ttgcgaacgt ttggcgacat gccgatgctg
     6361 tgcagaatga aatccagttt acctcccagc ctttccacac tttcatctat gagtttttcc
     6421 aggtcttcca tattggtggc atcagccgga atgatagtgg tattgcactt ttctgccagt
     6481 ttgttgatct cacccatacg catggcgatg ggggcattgg tcagagtgaa agtggcccct
     6541 tcttcaaagc acttctcagc cactttccag gcaatggaac tttcatcaag agcgccaaag
     6601 atgatccctc tttttccttt cagcaattgg ttagacatag gttgggttat tggttacgtt
     6661 tttttatttt ttttctgttc tttttgacag cccttttttc atccagggca cgggtgtact
     6721 gttcagaaaa attcaggtac atggcttcta ctgaagcctt atcctgcggg gcatcaataa
     6781 ggctgtaggt gccgttctgc ttgatcagtg tcagttttcc attttcatac acatagcgtg
     6841 cgttgaagga ggggctgatg ctgtcaaccg gcatgcccgg gtcataggca ttgtacacac
     6901 ggtgtacttc tgtaaccgtg atcagttctc ctttccaata gaaaaaagta cgctgaatat
     6961 ctccataact gaaaccaaac caggctttca tttccatgag ggtatcatgc tcatagatac
     7021 ctgaaagtgt agctccgaat tccggcttgg aattggtaaa agaggcatca ttgatctcta
     7081 ccctgtaagg ctttgctttc ttctgacggg aagcttgctg cccgaaaaca ggaaaggcta
     7141 tcaggaggat cagcgtacat agaatagtat tgcgcatcgt tccaaaaata aacatttgaa
     7201 gctatttgac cgttattttt agccgatgaa caccggcaga gatacttccc gcagccgtct
     7261 tttcagagaa ccggtctggc tcatgatcca cctggtgctg ctgggtatta tcatattgtt
     7321 tgcaggcctt actgcggcct acctgatcgg tccggatgca ggtgaagagg ccggttttcg
     7381 cataccccga atcttctggc tcagcagtat cctggtttta ctggtgtcac tgtccatgcg
     7441 tcgggtgttg aaagcctatg agcgtgataa cagttctaca ctgtcaggat ggttacttgc
     7501 agcaaccgta actgcattgc tttttgttgt ttgtcaatgg attggctgga caggtttgca
     7561 tcagcagggt attacccttc aggatacgcc ggctgcaggc tacctgtatg tgctgtccgg
     7621 gttacatgta gtgcatgtgg gtgtaggcat catcttgctc ctggtttctc tgacaaaaag
     7681 ccgaaggttg ctgcaacatg ccgccacagc actggtatat ttcagtgatc ctgtgcgcag
     7741 gcgaaggctg gagctgcttg ccagatactg gcataccatt gactatctgt ggttgttcct
     7801 gtttcttgta tttctataca aacatgcatg aaaaaacccg cctgcgggcg ggttttttca
     7861 tgcgctatta ttgaccgttc ttctttcttt tcttttcctg catcttttgc tgcatggcat
     7921 cctttcttgc agggtcttca ttggctctgg cccgcatctg gtctttacgg tctgccccct
     7981 cacgctgctc catacgatgt tcgcgggcag cttctttggc agcatcttta gagccatagt
     8041 gatcgatcat ggcctctttc ttttcctggc ggtcattacc gtctgcactt gctgcatgat
     8101 gttgtttcac ttctgctttg gagcgaccac tttcactgta tttttcagtc atatggtctt
     8161 tcttttgttg cttggtcacc tggcctgcat tctgggcata agcagaagca cccaggaaca
     8221 tcagtccggc agttatcagc atggtcagtt tcttcatgat cttgtttttt agtatgttca
     8281 gagtaatgct aaaagtgtgc caaaataaca aaagccaggc tacaatttac ccggattgcg
     8341 aaaatgcggg ggaaatcaga taattttgag ccccgtttcg ggatgtagct cagtccggta
     8401 gagtactcgt ctggggggcg agtggtcgct ggttcgaatc cagtcatccc gacaatcaca
     8461 tctgactttc ga
//
LOCUS       DAOMAX010000103         8429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1233_length_8429_cov_4.529496, whole genome shotgun sequence.
ACCESSION   DAOMAX010000103 DAOMAX010000000
VERSION     DAOMAX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_8429_cov_4.529496"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK742_06810"
     CDS             complement(<1..534)
                     /locus_tag="PK742_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76336.1"
                     /translation="MNRAVVYIGLFASITLFAVTVSAQLSAVPFNGGNGEGSAVASSI
                     YSEADSCYFTGSILPDTLIDCSYESTILDGSVYFAENYFWSTGDTTPVIAVDSSGWYS
                     VESSFGNCAIADSVYVWRSSEPLLSVVISDASSPEIADGFIAVDVLSATFPIDVYWIP
                     GPNDYLLDSILPGWYQFF"
     gene            complement(527..1993)
                     /locus_tag="PK742_06815"
     CDS             complement(527..1993)
                     /locus_tag="PK742_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQU76337.1"
                     /translation="MTCALRKYLILIYLLLSSGYLLLANGMEVSNVRLVNRDTTANTI
                     WILADIHWNNAWHLDAANAPGNYDAAWVFVKVRANNGASMHLLFELDDIVTPVNAHIE
                     VPPDKMGFFISLNDTTSGNAAFFDIAFRWNYDDYGISDTAEISAKVFAIEMVRIPEGA
                     FVAGTSAGGGEVDPFTPTTINTGLSETIPTGTDGIDGTPSGGFPAGEVAPAYNNFPNG
                     YKSFFCMKYEMSQQQYADFLNDLTPIQASQCFQPLETFDKSPFRYDIHLDSVSGLYVS
                     GNPYVALNVLSYADGAAYADWAGLRPMTELEFEKICRGPESSFPGQFPWGDSTITNLD
                     YTLINEGTLSEQLNLLDLSQGNAMYKAVNGVINGPVRCGAFAASSPIANRKITGAAYY
                     GVMEMGGNVLERVISLEFPEGRNYKGSHGDGYLTTDGFHTNADWPSASLPVGSSGRGG
                     KWNVEKDRMLISDRLLIGSHLGSRYHNAGFRFVRTSYE"
     gene            2059..3198
                     /locus_tag="PK742_06820"
     CDS             2059..3198
                     /locus_tag="PK742_06820"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HQU76338.1"
                     /translation="MKFNTKAVHAGVDPDPSTGAIMTPIFQTSTYVQAAPGDHKGYEY
                     ARTQNPTRHALEKSLAALENGSHAVCFSSGMAAVDAAIKLLDQGDEVISTNDLYGGTY
                     RSFTKIYSRFGLGFHFTDLTNPENILPLINSNTKMIWVETPTNPMLNIIDIEAVAAIA
                     RGHGLLLVVDNTFASPYLQNPLDLGADIVLHSATKYLGGHSDVVHGTLIVKDPNLAER
                     LFFIQNGNGAVPGPQDCFLVLRGIKTLHLRMQRACENAARIARFLHDHPKVDKVYWPG
                     FEHHPNHAIAAKQMRDFGAMVSFTLHGDDYDEALRVLSNTHLFSLAESLGGVESLIGH
                     PASMTHASIPKEERLKTGLVDSLIRLSIGIEDGDDLIEDLQKAIG"
     gene            3200..3976
                     /locus_tag="PK742_06825"
     CDS             3200..3976
                     /locus_tag="PK742_06825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HQU76339.1"
                     /translation="MDFRTLSLLALAIGPAIAIMAFVYARDVHDREPFGVLLMSFFWG
                     CFSVVPAIILETILPGLVPGSSGSGILSIAIYTFVIIAFSEEFSKYIFLRYYSYRKRS
                     FNEPFDGIIYMVMVGMGFATIENLMYVFGADTPQNQWATAGMRAITAVPAHASFAIVM
                     GYYAGLAKFSKDKEKQLLMQGILYATILHGFYDFFLFQNISEGIAIGALVSLIIGIRF
                     SLKAMRMHRDSSPFRKPATSEAITSEDEDEGHFESPYKPS"
     gene            3979..4755
                     /locus_tag="PK742_06830"
     CDS             3979..4755
                     /locus_tag="PK742_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HQU76340.1"
                     /translation="MADKRIVDLLQSAASLYNNPSFIQEDPISIPHSYNTLQDIEISG
                     FWTALLSWGQRPVIIRNARHLFHLMDDAPYDFILHHQESDLKRFLDFRHRTFQPTDTL
                     YFIHFFRWYYQQFDTLETAFLISGHALHLTTEHMLDHFHQLFFSLEDAPQRTRKHVPH
                     PSGKSTCKRLNMFLRWMVRDDGKGVDFGLWKNIRPDQLVCPLDVHVERVARKLGLITR
                     KQTDWQAAVELTANLRRIDPDDPVRFDFALFGLGVQERMA"
     gene            4796..5131
                     /locus_tag="PK742_06835"
     CDS             4796..5131
                     /locus_tag="PK742_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76341.1"
                     /translation="MQSALLQVCRYAEIEPDEQQLTDIVNEDMLQAKLEELVSWLLDK
                     DFEKLLWILYRVDVDEQRTKEILAQHVPADAPHILATLILERQKQKEEIRRSFQKAPV
                     ADEDEDLLL"
     gene            complement(5128..5610)
                     /locus_tag="PK742_06840"
     CDS             complement(5128..5610)
                     /locus_tag="PK742_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQU76342.1"
                     /translation="MEVLLLILGLVCMLVGLVGSVLPVLPGLPICYLGFLLLQWSAFG
                     EFSSYFLIAWAVIILIMVVIEYLIPKWGTKKYGGTRAGQNGALVGTIVGVFVFPPLGL
                     LLGPFVGAYVGELIDDSENNDKALRSAWGSLVGFMAGSVLKVTVALVMLFFFIREWIL
                     "
     gene            5659..6927
                     /gene="fahA"
                     /locus_tag="PK742_06845"
     CDS             5659..6927
                     /gene="fahA"
                     /locus_tag="PK742_06845"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HQU76343.1"
                     /translation="MSRANDPGLQSWISVPEESDFPIQNLPFGIFQTDGLSPRVGVAI
                     GDQVLDLAALNRDGFLTAFDLPDSILENQYLNDFIKLGKDKTSAVREHLSDLLQEENA
                     VLRDAPDALNKYFHLQASVQMLLPVQPGDYTDFYSSEVHATNIGRMFRPDNPLMPNWK
                     YIPVGYHGRSSTIIVSGEHFHRPKGQILEKDGEPPVFRASTQLDFELETAFITFDGPA
                     MGQSITTSEAEQYIFGMVLFNDWSARDIQRWEYVPLGPFLAKNFASSISPWVVTLDAL
                     QPFYCEGPVQDPPVLDYLRFNGPKNIDISLEVGIIPEGNEETTVCHSNFKYMYWNINQ
                     QLAHQTVNGCLVRAGDMYGSGTISAPAEDGYGSMMELTWRGQNPLTMKDGSTRKFIED
                     GDTVVIRGWCERNGIRVGFGEVSSKVLPAI"
     gene            6937..7791
                     /locus_tag="PK742_06850"
     CDS             6937..7791
                     /locus_tag="PK742_06850"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQU76344.1"
                     /translation="MLRIVPEEVKVPLRHQYMLGAISPRPIAFVSTIDAGGNPNLSPY
                     SFFNAVGSNPPTVIFSPALSGRDAKTKDTLLNIRETGEAVINIVSYSMVQQMSLASSP
                     YERGVNEFEKSGFTALASEIVRPFRVAESKVQMECIVQDIIETGTQGAAGNIVIAEIV
                     LMHIDESVLDENGRIDPYKMDYVARMGGNYYTRVIPESIFELVQPKDTCGLGVDQLPE
                     QIRLSKRLTGNHLGALGNFLTHPTPAEVADMLSAHPEWTDPADKETLAISLLENNQAW
                     EAFCILVA"
     gene            7795..8385
                     /locus_tag="PK742_06855"
     CDS             7795..8385
                     /locus_tag="PK742_06855"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQU76345.1"
                     /translation="MAPKTVESFLESNGQWQKTLTILRDLACSTGMEEAIKWGIPVYS
                     YKGKNVLGISAFKSYAGLWFYQGALLPDEKKVLMNAQEGKTKAMRQWRFGPGEKIPKP
                     TVLRYMREAMKLVDDGKEIKPEKNKALEIPTELQQALAGNKALQQAFGSLSLSHKREY
                     ADYIAEAKQTITRERRLKKITGMILKKTGLHDHHRK"
BASE COUNT         2387 a         1992 c         1952 g         2098 t
ORIGIN      
        1 aaaaaactgg taccaacccg gaagaatact atccagcaga tagtcattag ggccgggtat
       61 ccagtaaaca tctataggga atgttgctga caaaacgtcc acagctatga aaccatcagc
      121 aatttcaggg gatgaagcat ctgaaatgac cacagacagc aatggttcag aagatctcca
      181 tacatataca ctgtctgcaa tggcacaatt tccaaaagag ctttccacag aataccagcc
      241 agatgaatca acggcgatca ccggagtagt atcgccggtt gaccaaaaat agttttctgc
      301 aaaatatact gaaccatcca gtattgtgga ctcataagaa caatcaatga gggtatccgg
      361 caggatactg ccggtaaaat agcaactatc agcttctgag taaatggatg aagcaaccgc
      421 agaaccttct ccattaccac cgttaaatgg cactgcagaa agctgtgcag atactgttac
      481 tgcaaacaat gttatactcg caaaaagacc tatataaaca acggctctat tcatatgagg
      541 ttcttacaaa tctgaatcct gcattgtggt aacggctgcc cagatgactt ccaatcagaa
      601 gtctgtctga aatgagcatt ctgtcttttt ctacattcca ttttccaccc cttccggaag
      661 accctacagg aagtgaagca gatggccagt ctgcattggt atgaaatcca tcggtggtca
      721 gatagccatc accgtgtgaa cctttatagt tccggccttc aggaaactca agagagatga
      781 ccctttccag aacgtttcct cccatctcca taacaccata ataggcggcc cctgtaattt
      841 tcctgttggc aatcgggctg ctggctgcga aagcaccaca tctgactggg ccattaataa
      901 cgccatttac agctttatac atggcatttc cctgagacag atccaacaag ttcagctgtt
      961 cactcagcgt accttcattg atcagtgtat aatccagatt tgtgatggtt gagtcgcccc
     1021 aaggaaactg accggggaaa gaactttccg gacctctgca aattttttca aactccagtt
     1081 ctgtcattgg ccgcaaaccc gcccagtcag cgtaagcggc accgtctgca taactgagta
     1141 cattgagtgc tacatacgga ttgccgctga catataaacc agacactgag tccagatgaa
     1201 tatcgtatct gaaaggtgat ttatcaaaag tctccaaagg ttgaaagcac tgtgatgcct
     1261 ggatcggagt aagatcgttg agaaaatcag catattgctg ctgactcatc tcatatttca
     1321 tgcagaagaa tgatttatac ccgttgggga aattattgta ggcaggagca acctctccgg
     1381 ccggaaagcc cccggaaggt gtaccatcta ttccgtctgt gccggttggg atggtttcgc
     1441 tcaatccggt atttattgtt gtgggagtaa aagggtcaac ttcgccgcct ccggctgatg
     1501 ttcctgccac gaatgcccct tcaggtatcc tgaccatttc aatggcaaag accttagctg
     1561 atatttcagc tgtatcagaa attccataat catcgtagtt ccaccgaaaa gcgatatcaa
     1621 aaaatgcagc attgcctgag gtggtatcat tcagactgat aaaaaatccc attttatcag
     1681 gtggcacctc tatatgggca ttgacaggcg tcactatgtc atccaactca aacaacaggt
     1741 gcatggaagc tccgttgttt gccctgacct taacaaatac ccatgcagca tcataatttc
     1801 ccggggcgtt ggcagcatcc agatgccatg cattattcca atggatatct gcaagtatcc
     1861 atatggtatt tgcagttgta tctctgttca ccagccgaac attgcttacc tccatgccat
     1921 ttgccagcaa caaatagcct gaagagagca gcaggtaaat taaaataaga tactttcgga
     1981 gagcgcaagt cattacagca tttaaaagta tgcttcaaat gcgcttaaaa aaataccaat
     2041 tttgcacaaa atgtgcctat gaaattcaat accaaagccg ttcatgcggg tgtagatccg
     2101 gacccttcca ccggagccat catgacaccc atcttccaaa cctccaccta tgtgcaggcg
     2161 gcccctggcg atcataaagg gtatgagtac gcccggacgc aaaatcccac aagacatgct
     2221 ttggaaaaaa gccttgctgc tctggaaaac ggaagtcatg cggtctgttt ctccagtggc
     2281 atggcagctg tagatgccgc aatcaagcta ctggaccagg gagatgaggt catctccacc
     2341 aatgatctgt acggtggcac ctaccgttcc ttcacgaaaa tatattcgcg tttcggactg
     2401 ggttttcatt tcactgattt gaccaatcct gagaacatac ttccgctcat caacagcaat
     2461 accaaaatga tctgggtaga aacacccacc aatccgatgc tgaacatcat tgatattgag
     2521 gcggtggcag ccatcgccag ggggcacggt ctcctgctcg tggttgacaa cacgtttgct
     2581 tcaccctatt tacagaaccc gttagatctt ggggccgata tcgtattgca cagtgctacc
     2641 aagtacctgg gcggacatag tgatgtcgtg catggaacgc tgatcgtaaa agatcctaat
     2701 ctggccgaac gattattctt tattcaaaac ggcaacggag ctgttccagg accacaggat
     2761 tgttttcttg tactcagagg aatcaaaaca ttacatcttc gtatgcaaag agcctgcgaa
     2821 aatgcggcaa gaatagcccg tttcctccat gatcacccca aggtggataa ggtttactgg
     2881 ccgggttttg aacaccatcc caatcatgcg atagctgcaa agcaaatgcg tgatttcgga
     2941 gcgatggtct ccttcactct gcatggagat gattatgatg aagcgctgcg agtgctgagc
     3001 aatacgcatc ttttctcact ggctgagtca ttgggcggag tagagagcct gatcgggcat
     3061 cctgccagta tgacccatgc atcaatacct aaggaagaac gattgaagac cggtctggta
     3121 gattccctta tccgtctgag cataggtatt gaagatggtg atgatcttat tgaagacctt
     3181 caaaaagcaa ttggataata tggatttcag aacactgtct cttctggctc tggcaatcgg
     3241 tcctgccata gcgatcatgg cttttgtata tgcccgcgat gtacacgacc gagaaccctt
     3301 cggcgttctg ctgatgagtt ttttctgggg gtgtttcagc gtggtgcctg ccatcatact
     3361 ggaaacgata cttccaggac ttgtaccagg cagcagcgga tcaggcattc tgtccattgc
     3421 catttatact ttcgtgatca ttgcattcag tgaagagttt tccaaataca tttttctccg
     3481 ttactacagc taccgaaagc gttctttcaa cgaacctttt gacgggatca tctatatggt
     3541 catggtaggt atgggctttg caaccattga aaacctcatg tatgtttttg gtgcagacac
     3601 tcctcagaac caatgggcaa cagcaggcat gcgtgccatt actgcggtac ccgcacatgc
     3661 cagtttcgct attgtgatgg gatactacgc cggacttgcc aaattcagca aagacaaaga
     3721 gaagcagttg ttaatgcaag gaatcctgta tgccactatc ctacacggtt tttacgattt
     3781 cttcctgttc cagaatatca gtgagggtat tgccatcggt gcattggttt cacttatcat
     3841 tggaatcaga ttctctctga aggccatgcg tatgcacaga gattcttccc ctttcagaaa
     3901 gcccgcaact tctgaagcaa ttacgtctga agatgaggat gagggtcatt ttgaatcgcc
     3961 ttacaagccg agctgatatt ggcagataaa cggatcgtag atttattaca atcggcagca
     4021 agcctgtata acaatccatc atttatacag gaggatccga tcagcattcc gcacagttac
     4081 aacactctgc aggacataga gatcagcggt ttctggacgg cacttctttc ttggggacag
     4141 cgtccagtca tcatccgcaa tgcacgacat ctgtttcatc tgatggatga tgccccttat
     4201 gattttattc tgcatcacca ggaatcagat ctaaaaagat ttctggattt cagacaccgt
     4261 acctttcagc caacagatac attgtatttc attcatttct tcaggtggta ctaccagcag
     4321 tttgataccc tggaaacagc atttctgatc tctggccatg cacttcatct gacaacagag
     4381 cacatgcttg atcattttca tcagttgttc ttttcgctgg aggatgcacc tcaacgcacc
     4441 aggaaacatg tgccccatcc ctcaggaaag agcacctgca aaagactgaa catgtttctg
     4501 agatggatgg taagagatga cggtaagggt gtggacttcg gactctggaa gaatatccgt
     4561 cctgatcagc tggtatgtcc gctggatgtc cacgtagaac gggtagccag gaaactggga
     4621 ttgatcacca ggaagcagac cgactggcag gccgcagttg agctgactgc aaaccttcga
     4681 agaatagatc ccgatgaccc ggtaaggttt gatttcgccc tgtttggtct tggggtgcag
     4741 gaaagaatgg catgattttt ccgttatgat ggatatgcaa tcttcagaat cctggatgca
     4801 gtctgcactg ttacaagttt gccgatatgc cgagatcgaa ccagacgagc aacaactcac
     4861 agatatcgtt aatgaggaca tgctgcaggc taagctggaa gagcttgtaa gctggctgct
     4921 ggataaggat tttgaaaaat tactgtggat tttataccgc gttgatgtgg atgaacagcg
     4981 caccaaagag atcctggcgc aacatgttcc ggccgatgca cctcatattc tggccacact
     5041 gatactggaa cgacaaaaac aaaaagagga gatccggaga tcctttcaaa aggcaccggt
     5101 tgcagatgaa gatgaagact tgctgcttta aaggatccac tctctgataa agaagaacag
     5161 catgaccagc gccactgtca ctttcagcac agaacctgcc ataaacccaa cgagtgaccc
     5221 ccaggccgat cgcaacgctt tatcattgtt ttcactgtca tctatcaact cgcccacata
     5281 ggctccaaca aatggtccga gcagtaatcc cagtggcggg aaaacaaaca caccaacaat
     5341 ggttcccaca agtgctccgt tctggccagc cctggtgcca ccatattttt ttgttcccca
     5401 ttttggaatc aggtattcaa tgaccaccat gatgaggatg ataacggccc atgcgatcag
     5461 gaaataagaa gaaaattcgc cgaaggcaga ccattggagc aacaaaaatc ccagataaca
     5521 aatgggaaga ccgggtagta ccggcaacac ggaaccaacc agcccaacaa gcatacaaac
     5581 aagacccagt atcaatagca gcacttccat gtgcttcaaa gatagcttcc gtacctttgc
     5641 gcttgcaaac tggagtttat gagcagagca aatgaccccg gactacagtc ctggatatca
     5701 gtaccagaag aaagcgattt ccctatccag aacctgccat ttggcatctt tcagacagat
     5761 ggtctgtcgc cacgggttgg ggttgccatc ggagaccagg tgctggacct cgcagccctg
     5821 aatcgcgatg gattcctgac agcatttgac ctgcccgatt ccattctgga gaatcagtat
     5881 ttaaacgact ttattaaact gggaaaagac aagacctctg ctgtcaggga acatttgtca
     5941 gatcttttac aggaagaaaa tgcagtacta agggatgcac ctgatgcctt gaataaatac
     6001 tttcatctac aggcgtcagt acagatgctg ttaccggttc aacccggtga ttataccgat
     6061 ttctattcca gtgaagttca cgctaccaat attggcagga tgttccgtcc tgataatcca
     6121 ctcatgccaa actggaaata tattccggtt ggatatcatg gaagaagttc taccatcata
     6181 gtttccggag agcattttca tcggccgaag ggtcagatcc tggagaagga cggagaacct
     6241 ccggtcttca gggccagcac acaactggac tttgaactgg aaactgcttt catcactttc
     6301 gacggacctg ccatgggtca gtctataaca accagtgaag cggagcaata tatcttcggt
     6361 atggtgctat tcaatgactg gagtgcaaga gatattcagc gctgggaata tgtgccactt
     6421 ggcccattcc tggcaaagaa tttcgccagc agtatttcac cctgggtggt cacgcttgac
     6481 gctctgcagc cattctattg tgaaggacct gtacaggacc cgcctgttct ggactatctg
     6541 cgattcaacg ggccaaagaa tatcgacatt tcacttgaag tgggcatcat tcctgaagga
     6601 aatgaagaaa ccactgtatg tcattccaat ttcaaataca tgtactggaa catcaaccag
     6661 caactggctc atcagaccgt gaatggttgt ctggttcgtg ccggcgatat gtatggcagt
     6721 ggtaccatca gtgctcccgc agaagatggt tacggcagta tgatggagct cacctggcgt
     6781 ggacagaacc cactgaccat gaaagacggt tccacaagaa agttcatcga agacggagac
     6841 accgtggtca ttcgcggttg gtgtgaacga aatggaattc gtgtaggttt tggagaagtc
     6901 agcagtaaag tattacctgc aatttaacct gatatcatgt tgagaattgt tcctgaggaa
     6961 gtaaaggtgc cgttgcggca tcagtatatg ctgggtgcta tcagcccaag gcctattgca
     7021 tttgtaagca ctatagatgc cggtggcaat ccgaaccttt caccgtacag cttctttaat
     7081 gcggtgggca gcaatccgcc aacggtcata ttctcaccag ctctgagtgg cagagacgca
     7141 aagacaaaag acactttgct gaacatccgt gagaccggtg aagccgtcat caatattgta
     7201 agctacagca tggttcagca gatgtcgctg gccagttcac catatgaacg aggtgtcaat
     7261 gagtttgaaa agagcgggtt cactgcgctg gcatcagaga tcgtcaggcc gtttcgggtg
     7321 gcagaatcaa aggtacagat ggaatgcatt gtgcaggata tcatagaaac gggtacacag
     7381 ggagcagcgg gaaacattgt cattgctgag atcgtgctga tgcatattga tgaatcggta
     7441 ttggacgaga atggcagaat agatccatat aaaatggact acgttgcacg gatgggtggc
     7501 aactactata cccgtgtgat accggaaagc atatttgaac ttgtacagcc aaaagatacc
     7561 tgcggacttg gagtagatca gttgcctgaa cagattcgtt tgagcaaaag acttaccggt
     7621 aaccacctgg gtgcactggg caacttcttg acacatccaa caccagcaga ggttgctgat
     7681 atgttgtcag cacatccgga atggaccgat ccggcagata aagaaacact ggccatttct
     7741 ctcctggaga acaatcaggc ttgggaggct ttttgtattt tagttgcctg aaacatggct
     7801 cctaaaaccg tagaatcatt tcttgaaagt aacggacaat ggcagaaaac actgaccatt
     7861 ctgcgtgacc ttgcctgcag cacaggcatg gaagaggcca tcaaatgggg tattcctgtg
     7921 tactcttaca aagggaaaaa tgtacttggc atcagtgcct ttaaatccta tgcaggattg
     7981 tggttctatc agggggcatt actcccggac gaaaaaaaag tactgatgaa tgcccaggag
     8041 ggaaaaacaa aagccatgcg gcagtggcgc tttgggccag gtgaaaagat ccccaaacca
     8101 acagtgctgc gatatatgcg ggaagccatg aaactggtgg atgatggcaa agagatcaaa
     8161 cctgagaaaa acaaagccct ggaaattcct actgaattac aacaggctct cgcagggaac
     8221 aaggcattac aacaagcctt cgggtctttg tctttgtcgc acaaacggga gtatgctgac
     8281 tacattgccg aagccaaaca gactatcaca cgtgagagac gcctgaaaaa aataaccgga
     8341 atgatcctaa aaaagaccgg acttcacgac caccaccgga aatgagatct ttatttacca
     8401 gaactgttcc tgtcatactt actgctttg
//
LOCUS       DAOMAX010000104         8385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1244_length_8385_cov_3.860144, whole genome shotgun sequence.
ACCESSION   DAOMAX010000104 DAOMAX010000000
VERSION     DAOMAX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_8385_cov_3.860144"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK742_06860"
     CDS             <1..389
                     /locus_tag="PK742_06860"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQU76346.1"
                     /translation="FALGAYMLSAAIGMLLLETINYVEHYGLRRRKLDNGRYERVMPW
                     HSWNSNQYLGRIILYELTRHSDHHYLASREYQILRHMDEAPQLPAGYPAMMLLALLPP
                     AWFRVMDPRVEAVMAAHPVEPENEGL"
     gene            386..997
                     /gene="udk"
                     /locus_tag="PK742_06865"
     CDS             386..997
                     /gene="udk"
                     /locus_tag="PK742_06865"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQU76347.1"
                     /translation="MIVIGVCGGSGSGKTTFVNKLREALEVPHLPVLPQDAYYRDSSH
                     LPLEVRKKQNYDHPDSIEWELMSAHLGELLSGQTVPVPTYSMLTCTRTEEITHMHPTD
                     ILVVEGILIYTHAALREQIHLRVFLDVDEAHRYERLERRDMKQRGRTAEEVRERYLAT
                     VAPMYDEHIWPSRLHANLIVPKGGKNERAAGLIAGMVKGMRGA"
     gene            1121..1768
                     /locus_tag="PK742_06870"
     CDS             1121..1768
                     /locus_tag="PK742_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU76348.1"
                     /translation="MKHTALLLAGVCLLLSVSASAQKYPLGKGNLRSGGGFNFTQLDN
                     PDAKTYRFNVNPNLGYFVLDELLLGFNMNYSGTFGPEQYTGTLRFSPQVRYYYAINKS
                     WFLVGDFYYNMDRQRKMLGGATELDDNSSISYGPGFDYFLTRRISCEGVLLYTNYLYP
                     DAQHVNKLAFNFGFNIRLPIKRKDKLENLEIYKDTGNPYDRQGDEDLPDYVPTDR"
     gene            complement(1899..4343)
                     /locus_tag="PK742_06875"
     CDS             complement(1899..4343)
                     /locus_tag="PK742_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76349.1"
                     /translation="MKKTLLLLLATCLTSLLQAQSGSVSGQITNEEGTPLSEVTVRLD
                     DRFTITGKDGLYQLKEVENGAHTISISYLGYESLSIEFEIVNNEEKKVDLRLRETVTD
                     LPEVVISSVSMTGGQRNLRNLPGSAAYISPKQLQMFQYSDITRTLRSLPGINIQEEDG
                     FGMRPNIGMRGTGVDRSGKITLMEDGILMAPAPYAAPEAYYFPTFGRMQAVEVLKGSS
                     QIQYGPYTTGGALNLISTQIPDHLTTNLLMSGSGYGGKQLHAWMGDTYERFAFLAETF
                     RISSDGFKELDNGGPTGFEKQDYLIKLRLQSAADARYLQSLTLKAGLTEEVSDETYTG
                     LTYQDYLTAPFRRYAGSQLDKMTTRHEQLSLRYSMVLSPSLQWEVTAYRNDFHRNWYK
                     LDKVTDSLGTAVSISSLLADPDSYTGQYEIITGGNTTGIGALQVKANNRTYYSQGAEA
                     VLHYSHNGVNWNQHIQAGIRVHEDQIDRYQWVDLYGMDQGIMELQMTGTAGSESNRLV
                     TADALAAYLQYTLRRGQWTLTPGLRLEDITKTEKNYGTLDPERTGADLEVSSNNTSTL
                     IPGLLAEYRMSDQWYLYTGVHRGFAPAGVDTGSLPESSVNTELGARYWSRHAQATAVI
                     FYNDYSNLLGVDMTSTGGLGTGQTFNGGAATVYGLECSLGADLLPATSNWQLPVQIAY
                     TFTKGYFDSSFESDLEEWEEVEAGDELPYLAPQQLYLQLSAIRDRWSVHSGTRYTADM
                     RAVAGQGAIPTEELVPAHVVSDLSVHYTFAPGASAWAGVNNLTNNTYIASIRPAGWRP
                     GMPRTFTIGVRTLLTR"
     gene            complement(4418..5857)
                     /locus_tag="PK742_06880"
     CDS             complement(4418..5857)
                     /locus_tag="PK742_06880"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQU76350.1"
                     /translation="MNFRLQAGAAESSFCNILVIRDESDINHPLLEGMSATLERKIRD
                     KDNGWITWYGKDEVRIIAILKDSNGKRDHELTEEARVKGAQALKKANYEKAATATVVR
                     VSDSVSTEEMEAFIEGMCLAGYQFLRYRTEKKENTVKTVGVENSGLDQGALDMIAAVT
                     DANFAARDLINEPVLQMDSVQLAAFAAESGKKHGFQVTVLNKQEIEKLGMGGLLGVNK
                     GSSVPPTFSVLEYKPEKAVHDKPFILVGKGVTYDTGGYSLKPGNFMATMKCDMSGAAA
                     VIGTMIAVASNELPIHLIGLVPSTDNRVNSDALVPDDIITMSDGTTVEVLNTDAEGRL
                     ILADALHYAKQYDPELVIDLATLTGAAAAITGNLGAALMGTDTKYREILINSGNATYE
                     RLWEIPYWKEFADMLKGDIADLKNIGGPVGGSATAGKFLEHFTDYPWLHLDIAGPAFL
                     KEDQDYKQKGAVGFGVRLLFHFFRNISLR"
     gene            complement(5910..7304)
                     /locus_tag="PK742_06885"
     CDS             complement(5910..7304)
                     /locus_tag="PK742_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQU76351.1"
                     /translation="MYAIVDIETTGGLAGRHRITEVAVVLHDGEKTTDTWSSLINPEQ
                     DIPPFITQLTGISNDMVETAPVFSDIAEELYQILKDRIFVAHNAGFDYAFLKAMFEEE
                     GYLFQNKKLCTVRLGRKILPGHSSYSLGNICRDLGITITDRHRAMGDAKATTTLFEII
                     MRSDSGTIIADTLKRHHGQQALPPHLDKSVIQELPKKPGVYYFHDNKGRLLYVGKARN
                     IRHRVKNHFNFADHDGHENKLREEIHDVTCTETGNEFTALLLELDEIKRKKPPYNKAQ
                     KFWSRNYCIFSYTAQDGYKHLAIERYKSSLQPLRVFPHFLMARAFLKEKSEAFRLCAR
                     FCHLQQVAGACYDRQTGHCQGACEGLEAPDAYNDRVDEAISSFHDDLGTFIITGKGRQ
                     DGETSVVLVEQGHYLGFGFVPDDTGALTTESIKEYIQWRPDHPDVQYLILRHLEKHPG
                     SRIIIPASSREASA"
     gene            7485..8288
                     /locus_tag="PK742_06890"
     CDS             7485..8288
                     /locus_tag="PK742_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQU76352.1"
                     /translation="MHPIAKPFRFLFTVWAAVVFVALLLITAPFFFLFSILLGKRSIG
                     PLLFLCRFIAWGLSILLVIPFRFHRNPNVDRKQAYVVVANHRSNFDAPIAAASTWGII
                     RFLAKKELLKIPVLGAIFRRTAVNVDRSSPESRKKSMEILAASLARGESIFLFPEGTR
                     NKTTDQTLISFKDGAFRLAIDLQVPVLPQVYTGTDRVMPNSPMLLRPGMVHVYYLDPV
                     STVGMTDEDVPRLREMVKEKVREKYESAVRSGSESGSGSESGSESEEWE"
BASE COUNT         1873 a         2013 c         2340 g         2159 t
ORIGIN      
        1 tgtttgcgtt gggcgcatat atgctttcgg cagccatagg aatgctcttg ctggaaacca
       61 tcaattatgt ggagcactac ggacttcgca gaaggaagct ggacaatggc aggtatgaaa
      121 gggtcatgcc ctggcattcc tggaacagta atcaatatct ggggcgcatt atcctgtacg
      181 agcttaccag acattctgat catcactacc tggccagcag ggagtatcag atactgcgtc
      241 atatggacga ggcaccacaa ctaccggcag gctatccggc catgatgctg ctggcactgc
      301 tgccacctgc ctggttccgg gtgatggacc cccgtgtgga agcggtcatg gcggcgcatc
      361 ctgttgaacc tgagaatgaa ggactatgat cgtaataggt gtatgcggcg gatccggttc
      421 cggtaagacc acttttgtga acaaactaag ggaagcactg gaggtaccac acttgcccgt
      481 gttgccgcag gatgcctatt accgcgacag cagccatttg ccattagagg tacgcaaaaa
      541 gcagaattat gaccatcctg acagtattga gtgggagttg atgtctgctc atctcggtga
      601 gctgctttcc ggacagaccg taccggtgcc tacctacagc atgctcacct gcaccagaac
      661 agaagagatc acccatatgc accccacaga tattctcgtg gtggagggca ttctgatata
      721 tacacacgct gcattgcgag agcagataca tctgcgggtc ttcctcgatg tggatgaggc
      781 acaccgttat gaaaggctgg aaagacgtga tatgaaacaa cgtggccgca cggctgaaga
      841 ggtcagagag cgctatctgg caaccgttgc ccctatgtac gatgaacata tatggccctc
      901 caggctgcat gccaatctga tcgttcctaa aggcgggaag aatgagaggg ctgccgggct
      961 gatagccggg atggtgaagg ggatgagggg agcttgaggg ggagtgggag tgagagtggg
     1021 agtgagagtg ggagtgggag tgagagtggg agtgagagtg ggagtgagag tgggagtgat
     1081 aactagcggg gttcgtatat ttaattgtca atagccactt atgaaacaca ctgcattgct
     1141 cctggcaggg gtatgcctgc tgctttccgt ttccgcctca gcccagaaat atccgcttgg
     1201 gaagggtaac cttcggagcg ggggagggtt caatttcacc cagctggaca atcccgatgc
     1261 caagacctac cgttttaatg taaaccccaa tctgggctat ttcgtcctcg atgagctgtt
     1321 gcttgggttc aacatgaatt actccggaac attcgggccg gagcaatata ccggcaccct
     1381 gcggttcagt cctcaggtca ggtactatta tgctattaac aagtcttggt ttctggttgg
     1441 cgacttctac tacaacatgg acaggcaacg aaagatgctt ggtggtgcta cagagctgga
     1501 tgataattcc agcatatcat atggccccgg ttttgattat tttctaaccc ggagaattag
     1561 ctgcgaaggg gtgctgctct ataccaatta cctgtatccg gatgcccagc acgtgaacaa
     1621 actggccttc aatttcggct tcaatatacg tctgcccatc aagcgaaagg acaaactcga
     1681 gaacctggag atctataagg atacaggcaa tccgtatgac aggcaggggg atgaagacct
     1741 gccggactat gttccgaccg accggtgatc taaaggagat ttattttaat ttttactttc
     1801 aataaatctt ataaagtact caaatacagt attttattta ttagtgagta aaaaaattga
     1861 acaccccttt ttacccaggt attttgccat gatcaccttc atctggtcaa gagtgttctg
     1921 actccaattg taaaagtccg gggcattccg ggtctccatc ccgccgggcg gatcgaagct
     1981 atataggtgt tgttggtaag gttgttgaca cccgcccagg cggaggcgcc gggggcaaat
     2041 gtatagtgca cgctgaggtc gctgaccaca tgggcgggaa ccagttcttc ggtcgggatg
     2101 gcaccctgtc cggcaacagc ccgcatatcg gccgtgtacc ttgttccgga atgcacagac
     2161 catctgtctc tgatagccga cagttgcaga taaagttgct gaggggcaag gtagggcagt
     2221 tcatctccgg cttccacttc ttcccattct tccagatctg attcaaagct ggaatcaaaa
     2281 taacctttgg taaaggtata ggctatctgg accggcaatt gccagttgct ggtagccgga
     2341 agcagatcag caccgagtga acattccagt ccatatacag tggcggcacc tccattaaag
     2401 gtctggcctg tacccagtcc gccggtgctg gtcatatcaa cgcctaacag gttgctgtaa
     2461 tcattataga atatcacggc ggttgcctgt gcatgccttg accagtaacg ggcaccgagt
     2521 tctgtattga cggacgattc cggcagggaa ccggtatcca ctcctgcagg ggcgaaccct
     2581 ctgtgtaccc cggtatagag ataccattga tctgacatgc ggtattctgc cagcagtccg
     2641 gggatcaggg tactggtatt gttggaagat acctccagat cagcccctgt gcgttccgga
     2701 tcaagggttc cgtaattctt ttcggtcttg gtgatgtctt ccagtctcag gcccggggtc
     2761 agggtccact gacctcttct tagggtatat tgcaggtatg ctgccaaagc atctgctgtg
     2821 accagtctgt tgctttcaga gcccgctgta ccggtcatct gcagttccat gataccctgg
     2881 tccatgccgt acagatctac ccactgatac ctgtcaatct ggtcttcatg cacccggata
     2941 cccgcctgga tgtgctgatt ccagtttaca ccattatgac tatagtgcag cacggcttct
     3001 gctccctggc tgtaataggt ccggttgttg gctttaacct ggagggcgcc gatgccggtg
     3061 gtatttcccc cggtaatgat ctcgtactga ccggtataac tgtcagggtc agccagcagg
     3121 ctggaaatgg aaacggcagt acccaggcta tcggtcacct tgtccagttt gtaccagttg
     3181 cggtggaagt catttctgta tgcggtcact tcccactgca gagagggaga caggaccatg
     3241 ctgtaccgaa gtgacagctg ttcatgtctg gtggtcattt tgtccagctg agaaccggca
     3301 tagcgtctga acggagctgt cagataatcc tggtaggtca gacccgtata ggtttcatca
     3361 gaaacttctt ccgtcaggcc ggctttcagc gtcaggctct ggaggtagcg ggcatcggcg
     3421 gcagattgca ggcggagctt gatcaggtag tcctgtttct caaatccggt cggtccacca
     3481 ttgtccagtt ccttgaatcc atcgcttgag atgcggaagg tttcagccag gaatgcgaat
     3541 ctttcatagg tatctcccat ccaggcatgc aactgcttgc cgccatatcc ggatccgctc
     3601 atcagaaggt tggtggtcag gtggtccggt atctgggtgc tgatgagatt cagtgcccca
     3661 ccggtggtat agggaccata ctggatctgg ctgcttcctt tcagcacctc cactgcctgc
     3721 attcttccga atgttggaaa gtaatatgcc tctggcgcag cataaggtgc cggtgccatg
     3781 aggatgccat cttccatcag ggtgatcttt ccgcttctgt ccactccggt gccacgcata
     3841 ccgatattcg gccgcatgcc gaatccgtct tcttcctgaa tattgatacc cggcagcgac
     3901 ctcagggtcc gggtgatatc tgagtactga aacatctgca attgcttggg agagatgtat
     3961 gccgcactgc ccggcaggtt gcgcagattg cgctggccac cggtcatact gacagagcta
     4021 atgaccactt cggggaggtc ggtcactgtt tcccgaagtc gcagatcaac ctttttttct
     4081 tcattgttaa ctatctcaaa ttcaatagat aaggattcat atcctaaata agagatagag
     4141 atagtatgcg caccgttctc cacctctttc aactgataaa gtccgtcttt cccggtgatc
     4201 gtgaaacggt cgtccagcct gacggtcacc tcactaagag gtgttccttc ttcattcgtg
     4261 atctgaccgg aaacacttcc ggattgggcc tgaaggaggc tggtaaggca ggtcgccagg
     4321 agcagtagta aggtcttttt catctattta gaatttgtct aaacagatgc aaagatgtac
     4381 caggcagggt ctgaattgca ataccctgag atgggtatta tcgcaggctg atattgcgga
     4441 agaagtggaa caggagtcgt accccgaatc ccacagctcc tttttgcttg tagtcctgat
     4501 cttctttcag gaaggcagga ccggcgatat ccaggtgcaa ccaggggtag tcggtgaaat
     4561 gttccaggaa ttttccggcg gtggcagaac ctccaacagg tccgccgata tttttcaggt
     4621 cggcgatatc tcctttcagc atatcggcaa actccttcca gtacggaatt tcccaaagtc
     4681 gttcataggt agcattgccg ctgttgatga ggatctcccg gtatttggtg tcggtaccca
     4741 tgagggcagc tcccagattt ccggttatgg ctgcggcggc accggtcagg gtagccaggt
     4801 caatgaccag ttcagggtcg tattgtttgg cataatgcag ggcatcagcc aggatgagcc
     4861 ggccttctgc gtcggtattc agtacctcta cggtggtacc atcgctcatg gtgatgatat
     4921 cgtcgggcac aagggcatca ctgttcaccc ggttatcggt agatggcacc agtccgatca
     4981 ggtgaatggg caattcattg gaggctacgg cgatcatcgt cccgatcacg gccgcagctc
     5041 cgctcatatc gcacttcatg gtggccatga aattgccggg tttcaggctg tagccaccag
     5101 tatcataggt gacacctttt cccaccagga tgaaaggttt atcatgtacc gctttttcag
     5161 gcttgtattc cagaacgctg aaggtgggtg gtaccgagct gcccttattg accccaagca
     5221 atccccccat tcccagtttt tctatctcct gtttgttgag cacggttacc tggaatccat
     5281 gctttttgcc tgattctgct gcgaatgccg ccagttgcac agagtccatc tgcagaaccg
     5341 gctcattgat gagatcgcgg gcggcaaagt tggcatctgt gacagctgcg atcatatcga
     5401 gagccccctg atcgagacca ctattctcta ccccaacggt ctttacggta ttttccttct
     5461 tctccgttct gtaacgaagg aactggtaac cagccaggca cattccctct atgaaggctt
     5521 ccatttcttc ggtagaaacg ctgtcggaaa cccgcaccac cgtggctgtt gccgcttttt
     5581 cgtagttcgc ttttttcaat gcctgagcac ctttgactct ggcttcttcg gtcaattcat
     5641 gatctctttt accattgctg tctttcagta tggcgatgat gcgcacttca tccttcccgt
     5701 accaggtgat ccagccattg tccttatcgc ggatctttcg ctcgagggta gcactcatgc
     5761 cttccagcag agggtggttg atatcagatt cgtcgcggat gaccaggatg ttacaaaagg
     5821 aagattcggc agcacctgcc tgcaggcgga aattcataga taaataattg aaactcaaag
     5881 atagggcatt ttagacgccc cgaaatgcat caggcagacg cctcccgtga cgaagcggga
     5941 atgatgatgc ggcttccggg atgcttttcc agatgacgga gtatcaggta ctgcacatcg
     6001 ggatgatctg gccgccactg gatatattct ttgatgcttt cggtggtgag tgcgcctgta
     6061 tcatccggca caaagccgaa ccccaggtaa tgcccctgtt ctacgagcac cacagaggtt
     6121 tctccgtcct gccttccttt tccggtaatg atgaatgtgc cgaggtcatc gtggaaactg
     6181 ctgatggctt catccactct gtcgttgtat gcatctgggg cttccaggcc ttcgcatgct
     6241 ccctgacaat ggccggtctg tcggtcataa caggcgcctg ccacctgctg gaggtgacaa
     6301 aacctggcac ataaacgaaa ggcttcagac ttttctttca gaaatgctct tgccatcaga
     6361 aagtgcggaa agaccctcag tggttgcagg ctgcttttat accgttcgat ggccaggtgt
     6421 ttgtagccat cctgtgcggt ataggagaag atgcagtaat ttctgctcca gaacttttgt
     6481 gccttattat aaggtggttt ctttcttttg atctcgtcga gttccaatag caatgctgtg
     6541 aactcatttc cggtctcggt gcaggtcaca tcgtggatct cttcacgcag tttgttctca
     6601 tgaccatcgt gatcggcgaa gttgaagtgg ttctttaccc ggtgcctgat atttcgggct
     6661 ttgcctacat agagcagccg gcccttgttg tcgtggaagt aatagacacc aggtttttta
     6721 ggaagctcct ggatcacaga tttgtccagg tgtggaggca gcgcctgttg accatgatgt
     6781 cgtttcaggg tgtcagcgat gatggttccg ctatccgacc gcatgatgat ctcgaacagg
     6841 gtggtagtgg ctttggcatc gcccatggcc ctgtggcgat cggtaatggt gatgcccaga
     6901 tcgcgacaga tgtttcccag tgaatagctg ctgtgtccgg gcaggatctt acgacccagt
     6961 cttacggtac agagtttttt gttctgaaag agatagcctt cttcttcaaa catggccttg
     7021 aggaatgcgt aatcaaagcc tgcattatgg gcaacgaaga tccggtcttt caggatctga
     7081 tagagttctt cggcaatatc ggagaatacc ggggctgttt ccaccatatc attgctaatg
     7141 ccggtcagct gggtgatgaa gggcggaatg tcctgttcgg gattgatgag tgacgaccag
     7201 gtatcggtgg tcttttctcc atcgtgcaac actacggcca cttcggtgat gcggtgtctg
     7261 cccgcaagtc ctccggtggt ctcgatgtca acgatggcgt acatggttat aaaggtaaaa
     7321 aggggagtga gaagtgagga gtgagtagtg aggagtggga gtgggagtgg gagtgggagt
     7381 caggaggggg agtggagagt gggagtggga ggagtgacgg ggaggtaaga taccacattg
     7441 aagcagatag gtgttccggt attgaattgt tattattgct ttcgatgcat cccatcgcca
     7501 aaccattcag attcctgttc accgtttggg cggcagtggt gtttgtggca ttgctgctga
     7561 tcactgctcc gttcttcttt ttgttttcga tcctgctggg aaagcgttct atcggtccgc
     7621 tgctgtttct gtgcagattc atcgcctggg gtctgagcat cctgctggtc attcctttca
     7681 ggtttcaccg gaatccgaac gttgacagaa aacaggctta tgtagtggtg gccaaccatc
     7741 gctccaactt tgatgcgccc atagcagctg catccacctg ggggatcatc cggtttctgg
     7801 caaagaagga attgctgaaa ataccggtac tgggtgcgat ctttcgtcgg acagccgtga
     7861 atgtggaccg gtccagcccc gaaagcagaa agaaaagtat ggagatcctg gcagcctcgc
     7921 ttgcaagggg agaatccatc tttctcttcc ccgaaggaac caggaacaag accaccgacc
     7981 agacactgat atcctttaaa gatggagcct ttcggctggc gatagacctg caggtgccag
     8041 tgttgcctca ggtgtacacc ggaactgaca gggtcatgcc gaacagtcct atgttgttac
     8101 gccccggtat ggtgcatgtt tattaccttg acccggtatc tacggttggt atgacggatg
     8161 aagatgttcc ccggttacgg gaaatggtga aggagaaggt gcgggagaag tatgaatcag
     8221 cagtgaggag tgggagtgag agtgggagtg ggagtgagag tgggagtgag agtgaggagt
     8281 gggagtgaga gtgaggagtg ggagtgagga gtgggagtgg gagtgagagt gggagtggga
     8341 gtgggagtgg gagtgagagt gggagtggga gtgagagtgg gagtg
//
LOCUS       DAOMAX010000105         8291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1265_length_8291_cov_4.089728, whole genome shotgun sequence.
ACCESSION   DAOMAX010000105 DAOMAX010000000
VERSION     DAOMAX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1265_length_8291_cov_4.089728"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..663)
                     /gene="frr"
                     /locus_tag="PK742_06895"
     CDS             complement(88..663)
                     /gene="frr"
                     /locus_tag="PK742_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQU76353.1"
                     /translation="MNDQLMEELDLIKGMAEEHMQKAVHHLEAELVKVRAGRANASML
                     EGVKVDYYGTHTPVQQVANISTPDARTIAIQPWEKHMLGEIEKAILAANIGLTPQNNG
                     EMVRLNLPPLTEERRKELVKNVKHLGEGARVTVRNARKEANDHIKKIQKDGLPEDMAK
                     DAEDLVQKMTDQYIAEVDKVLDRKEKEIMTV"
     gene            complement(674..1390)
                     /gene="pyrH"
                     /locus_tag="PK742_06900"
     CDS             complement(674..1390)
                     /gene="pyrH"
                     /locus_tag="PK742_06900"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQU76354.1"
                     /translation="MPVQYKRILLKLSGESLMGSQQYGIDAAMLQQYAAEIKQAAALG
                     VEIAIVIGGGNIFRGMRGTGSGIDRAQGDYMGMLATVINGMALQSALENAGMYTRLLS
                     AIKMEQICEPYIRRRAMRHLEKGRIVIFGAGTGNPYFTTDTAASLRAVEVEADVILKG
                     TRVDGIYTADPEKDPTATRYDKITFREVYQKNLNVMDMTAFTLCEENRLPIIVFDMNK
                     QGNLSALLSGEQVGTLVSND"
     gene            complement(1430..1948)
                     /locus_tag="PK742_06905"
     CDS             complement(1430..1948)
                     /locus_tag="PK742_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQU76355.1"
                     /translation="MRYKMIIGLALLVGILFLWGRNLQAQVHVSDKKYDIMLSTLLSP
                     GVPAVSVDSIEHGKYLLLDAREKREFEVSHIENAMWVGYDDFDLKRVAGISKEEYLAV
                     YCSVGYRSERITEKLREAGYLHVYNVYGGIFEWVNTGHPVVTDLDTPTEEVHAYSSSW
                     GVWLKQGRKVYE"
     gene            complement(1969..2814)
                     /locus_tag="PK742_06910"
     CDS             complement(1969..2814)
                     /locus_tag="PK742_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQU76356.1"
                     /translation="MERRPSGLRFFMEWTIIAGYAGALIMGITLGLLGAGGSILTVPV
                     LYYLFGVDAVLSTAYSLFIVGFTALAGAVPNMRKGLVHYQTAILFSIPSLIAVYLTRA
                     FLVPAIPDTVMTLGDWVLTKEMAILGFFAVIMVFAAISMLRRSHLEDPSSDSIGKRNP
                     APILLEGLVVGTLTGLVGAGGGFLIIPALVIFGKLPMKLAIGTSLLIIAIKSIFGFFG
                     DVQSGQEIDWSFLLLFTGITIVGILIGTRWTTFVEAGKLKKAFGWFVLLMGVYIIARE
                     LFLQA"
     gene            complement(2843..4378)
                     /locus_tag="PK742_06915"
     CDS             complement(2843..4378)
                     /locus_tag="PK742_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQU76357.1"
                     /translation="MSHHQTLEEIQDFKGKYPRQIWYLFFSEMWERFSFYGMRGMLTY
                     FMINQLLMEDKTANLQYGATQAFVYAFTFIGGLFADKILGFRKSLFWGGLLMITGSII
                     LAVDPHQFFFLGISFNIIGTGFFKPNISTMVGALYKDGDARRDAGFSLFYSGINIGAL
                     LGGYFCISIGKGTMLTSMVPEGATWNVAFGLVAVVMTISLLTFVYTRNTMATIGLSPL
                     RDASKPGNSIQASKRWYEYAVYAGGLISIPIIMKMVANPVYVDWFMYIVGPAALLWLF
                     YEMTKHSGAEVRRLFAALLFIVFSVIFWAIFEQAGGSLTIFAANNLDNELLGFIKLDP
                     NGVNNAANSLFVIFFAPVIGLIWIALAKKKKEPNTIVKFGLGFLFLGLAFYIFFGTRF
                     FATMEGIASLDVFTLAYFVVTLGELCLSPIGLSAMTKLSPARLQGLMMGMWFLASAYG
                     QYVAGLFGASIAGDVTTASNYDKLMIYTQGYKDMAIYAVIAGVILIIVAPFVRKRLMG
                     HVH"
     gene            complement(4553..6274)
                     /locus_tag="PK742_06920"
     CDS             complement(4553..6274)
                     /locus_tag="PK742_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQU76358.1"
                     /translation="MTAERKLAAHPKGLYVLFFTEMWERFGYYLMLGIFSLYMLDSLE
                     NGGMGFSGAKKSDIYGTYIGLVYLTPFIGGLLADRILGYRRSIFLGGSLMAAGYLGLS
                     IHNETAFYVSLLLIILGNGFFKPNISTLVGNLYNDPRYIANKDSGYNIFYMGINIGAF
                     ICNFVAAFLRNNYGWGFAFIAAGIGMIIGLIVFASGTRHVKEADTLKPVKPGDMGTGV
                     ILAATILPMFVFGIIGYFLKGVGGDGNIFGTDTNDAFIFGCIPVIGFFLYLMFSADKE
                     DRGPIAALLSVYGCIVVFWAVFHQNGDALTVWAEEYTDREMPAAIAKPANAIGMTQNV
                     TYFPELGMSEEAFTHHLDSLKVEASKLEAGSDASKELNAHIGSLETSKRYFENLPADE
                     RPAEGKDLKLVSTEIYQSVNPFWVIVLTPLVVGVFSWLRRRKKEPTTPGKIAIGLVIT
                     ALSALVMVMAVKSTNLDDEKASSMWLIATYGVITIGELCLSPMGLSLVSKLSPPRLTA
                     LMMGGFFLSTSVGNKLAGILSSNFEATENKDGFFLTNFALVMVAAVMMFLLLRWLNRV
                     MREKGLV"
     gene            complement(6320..7837)
                     /gene="nhaC"
                     /locus_tag="PK742_06925"
     CDS             complement(6320..7837)
                     /gene="nhaC"
                     /locus_tag="PK742_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HQU76359.1"
                     /translation="MDGNLYFGATNQTAFMDDRLHPDGQEPGILHALLPVLVLVGLLV
                     LNVLFFGDMATSGANQIALIIAAAVAAVMGLMLKVPLAHMIEGVVHSIMSAMNAILIL
                     LLIGALAGTWLISGVVPAMIYYGLQILNPTIFLFAACIICAVVSLATGSSWSTVATVG
                     IALLGIGQAMGVNEGWIAGAIISGAYFGDKMSPMSDTTNLAPAMAGTDLFTHIRYMVL
                     TTVPSMVITLLLFLFIGFFHMPEVSPENMNALGNAIHQSFHINIFLFIVPAVVIWMII
                     KKADPITALLIGSLLGGVVAIIFQPEVVAEVSGITGSYAKQSYMAVVNAMSGSISVSS
                     DNEIAAGLLSARGMAGMLNTIWLIVCAMCFGGVMESVGLLARITRPLVERAKTTGSLI
                     ATTATSCVFLNVTASDQYLAIVVPGRMFAKTFRQRGLAPQNLSRTLEDSGTVTSVLVP
                     WNTCGATQAGVLGVATLTYLPFCFFCIISPLMTILYGYLGIRIAKIPSDDQMATA"
     gene            complement(8024..>8291)
                     /locus_tag="PK742_06930"
     CDS             complement(8024..>8291)
                     /locus_tag="PK742_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76360.1"
                     /translation="TGIAGNILHVSFDANGEYLRFGPNQGAGGRYTVMAYDKNGVYGY
                     VMYGTQEFKLASYNNHYAHLLLDMERYDEITKERREVSGRTLSP"
BASE COUNT         2122 a         2199 c         1983 g         1987 t
ORIGIN      
        1 actcctcact cctcactcct cactacccaa tacccaatac ccaataccca atactcaata
       61 cccaatactc aatactcact accaaaatca caccgtcatg atctctttct cttttctgtc
      121 cagcaccttg tctacctcag ctatatactg atcggtcatc ttctgcacca ggtcttccgc
      181 atctttggcc atgtcttcgg gcaaaccgtc cttctggatc tttttgatat gatcgttggc
      241 ctccttgcgg gcgttgcgaa cggtgactct tgcgccttca cccaaatgct tcacattttt
      301 caccagttcc ttgcgccttt cttccgtaag aggtggcagg ttgagacgga ccatctcacc
      361 attgttctga ggtgtgagtc cgatattggc tgccaggatg gccttctcga tctcgcccag
      421 catatgcttt tcccagggct ggatagcgat ggtccgggca tcaggtgtgc tgatattggc
      481 tacctgttgt acgggcgtat gggtaccgta ataatctacc ttgacacctt ccagcatact
      541 ggcattggca cgacctgccc gcaccttgac cagttcggcc tcaaggtgat gaacagcttt
      601 ctgcatatgc tcttcagcca tgcctttgat caaatccagt tcttccatta actgatcgtt
      661 cataccagat tatttagtca ttactcacaa gggtacctac ctgttcgccg ctgagcagag
      721 cgctcagatt accctgttta ttcatatcaa atacaatgat gggcagacgg ttctcttcac
      781 acaaggtgaa tgcggtcata tccatcacat ttaaattctt ttgatatact tcacggaaag
      841 tgatcttgtc gtagcgtgta gctgtcgggt ccttctccgg atctgcggta tagataccat
      901 ccactctggt ccctttcagg atcacatcag cttctacttc cactgcccgg agagatgcag
      961 cggtatcagt ggtgaaatag ggattgcctg tacccgcacc gaaaatgacg atacgtcctt
     1021 tttccaggtg ccgcatggcc cttctcctaa tatagggctc acagatctgt tccattttta
     1081 tggcgctcaa caagcgggta tacatgccgg cattttcgag tgcgctttgt aaggccatgc
     1141 cgttgatcac agtggccagc atgcccatgt agtcaccctg tgcccggtca ataccactgc
     1201 cggttccccg catacccctg aaaatgttac ccccaccgat cacaatggcg atctcgaccc
     1261 cgagagccgc tgcttgcttg atctcagctg catattgttg cagcatggcc gcatcaatac
     1321 catattgctg actacccatg agtgactcac cactcagttt taataagatg cgtttatatt
     1381 ggacaggcat ggttgcaaat ataccacttt gcccgtgagc tgatgaggct tactcgtaca
     1441 ccttacggcc ctgtttgagc cagactcccc aggagctgga ataggcatgc acttcttccg
     1501 ttggagtatc cagatctgtc accaccgggt gaccggtatt gacccattca aagatgccac
     1561 cgtacacatt ataaacgtgc agatagccgg cttctctgag cttctcagtg atgcgttcac
     1621 ttcggtagcc gacagagcaa tacacagcca gatattcttc cttactgata ccggccactc
     1681 tcttcaggtc aaagtcatcg taccctaccc acatggcatt ctcgatatgg ctcacctcaa
     1741 attcgcgttt ctctcttgcg tccagcaaaa gatatttgcc gtgttcaata ctatcgaccg
     1801 aaacggcagg tactcctgga gataacaatg tggacagcat gatatcgtac ttcttgtcgc
     1861 tcacatgcac ctgcgcctgc agatttcttc cccacaggaa aagtattcct accagcagtg
     1921 ccagacctat gatcatttta tatcgcatcg gtattcttcg ccaaaacttc atgcctggag
     1981 aaacagttcc cgggcaatga tatagactcc catcagcagc acgaaccagc caaatgcctt
     2041 cttcagtttc ccggcttcta caaaggtagt ccatctggtt cctatcagga tacccacaat
     2101 ggtgataccg gtgaagagca acaggaagct ccagtcgatc tcctgaccac tctgcacatc
     2161 gccaaagaat ccgaatatgg attttatggc gatgataagc aaggaggtac cgatggccag
     2221 cttcatgggc aatttgccaa aaatgaccag tgccggtatg atgaggaaac ctccgccggc
     2281 gccaaccagt ccggtcaggg tgcctactac caatccttcc agcaaaatag gtgccggatt
     2341 tctcttccct atggaatcag atgacggatc ttccaggtga ctgcggcgca gcatactgat
     2401 ggcggcgaaa accatgatga cagcaaaaaa accgaggatg gccatctctt tggtcaggac
     2461 ccaatcgcca agcgtcatta ccgtatccgg aatagcgggc accagaaatg cccgtgtcag
     2521 atagaccgca atgagagatg ggatggaaaa aaggatggcg gtctggtagt gtacaagtcc
     2581 tttccgcata ttcggtactg cccctgccag tgcggtaaaa cccacaatga acagggagta
     2641 ggctgtgctg agaaccgcat ccacaccaaa taagtaatac aggacgggca ctgtgagaat
     2701 actaccgcct gcacccagca atccgagggt gatgcccatg atgagggctc ctgcatagcc
     2761 cgctatgatc gtccattcca taaaaaaacg caggcccgaa ggtctgcgtt ccaagttaca
     2821 agttatgtga ccaaccgcac acttagtgca cgtgacccat caggcgcttg cgcacgaatg
     2881 gtgctacaat gatcaggatc acacctgcta tcaccgcata tatggccatg tctttatagc
     2941 cctgtgtgta gatcatcagc ttgtcatagt tgctggctgt ggtcacatcg ccggcaatgc
     3001 tggcaccgaa cagacctgcc acatattgcc cgtaagcgga tgccaggaac cacataccca
     3061 tcatcagacc ctgaagtctt gccggactga gtttggtcat ggcagacaga ccgatgggag
     3121 ataagcaaag ctcacccagc gtgaccacga aatatgccag tgtgaataca tccagtgatg
     3181 cgatgccttc catggtagca aagaacctgg taccgaaaaa tatatagaat gccagtccga
     3241 ggaatagaaa cccaagaccg aatttcacaa tggtgttcgg ttcttttttc tttttggcca
     3301 gtgcgatcca gatgagacct atgaccggtg cgaagaagat cacgaacagc gagttggctg
     3361 cattattcac cccgttggga tccagtttaa tgaagcccag caattcgttg tccaggttgt
     3421 tggctgcaaa gatggtgagt gaccctccgg cctgttcaaa gatggcccag aagatgaccg
     3481 agaacacgat gaacaacaag gcggcaaaca atctgcgcac ttctgcacct gagtgtttgg
     3541 tcatctcata gaacaaccat agcaaagcgg caggaccgac gatatacatg aaccaatcca
     3601 cataaaccgg attggccacc attttcatga tgatgggaat ggagatcaga ccacctgcat
     3661 agacagcata ttcataccag cgcttggaag cctgaatgga attacccggc ttactggcgt
     3721 cacgcagcgg cgataaacca atagtggcca tagtatttct tgtatagaca aaggtgagga
     3781 ggctgatggt catgaccacg gccaccagac caaaggccac gttccaggtg gctccttcgg
     3841 gaaccatgga tgtcagcatc gtacccttgc caatggagat acagaaataa ccgcccagca
     3901 gggcgccgat gttgataccg gagtagaaca gggagaaacc tgcatcacga cgggcatcac
     3961 cgtctttgta cagcgcacct accatggtag aaatattcgg tttgaagaaa ccggtgccga
     4021 tgatgttgaa gctgataccg aggaagaaga actgatgcgg atcaacggcc aggatgatgg
     4081 aaccggtgat catcaaaagc ccgccccaga acagcgactt ccggaatccg aggatcttat
     4141 cggcaaacaa accgccgata aaggtgaatg catagacaaa tgcctgtgtg gctccatact
     4201 gcaggttggc cgtcttgtct tccatcaaca gctggttgat catgaagtag gtcagcatgc
     4261 cccgcatacc gtagaagctg aaccgctccc acatttcact aaagaacaaa taccagatct
     4321 gtctggggta tttgccttta aagtcctgga tctcttcgag agtttggtga tgactcatag
     4381 atagttgttt tgttggctcc ctgcctgagg gagggatttg gtgccgctaa agttaaaaga
     4441 tttcccggga ggcaagacct cctgatcttc cggcattcca aaaccatgcc cggaccacaa
     4501 aaaagggcgc agttttaaca ctgcgcccga tttttcttgc ttccgtatga ccttatacca
     4561 ggcctttctc ccgcatgaca cggttcagcc agcgcagcag caggaacatc atgactgctg
     4621 ccaccatgac cagagcaaag ttggtcagga agaatccgtc tttgttttca gttgcttcaa
     4681 aattgctgga gaggatgcct gccagcttgt ttccaaccga ggtggacagg aagaaacctc
     4741 ccatcatcag agctgtgaga cgtggtggtg ataatttaga caccagggaa agacccatcg
     4801 gactcagaca aagttcaccg atggtgatca ctccataggt agcaatgagc cacatactgc
     4861 ttgccttttc atcgtccagg ttggtgctct tcaccgccat gaccatcacc agtgcagaca
     4921 aggccgtgat gaccagacca atagcgatct tacccggcgt ggtcggctct ttcttccttc
     4981 ggcgcaacca gctgaaaaca cctaccacca gcggagtgag aacgatcacc caaaacgggt
     5041 ttacactttg gtagatctcg gttgatacca gcttgagatc cttaccttct gctggtcgtt
     5101 catctgccgg cagattctca aaatatctct tacttgtttc caggctgcct atatgtgcat
     5161 tgagttcttt ggaagcatcg ctgccagctt ccagtttgga agcttccact ttcaggctgt
     5221 ccaggtgatg tgtgaaggcc tcttcagaca tgccgagttc aggaaaatag gttacgttct
     5281 gggtcatgcc gatggcattg gcaggtttgg cgatggctgc aggcatctcc cggtctgtat
     5341 attcttcggc ccacacggtc agggcatctc cattctggtg aaagaccgcc cagaacacta
     5401 cgatacatcc atatactgat aacaaggcag cgatcggtcc gcggtcttcc ttatctgcag
     5461 aaaacatcag atacagaaag aacccgatga cagggataca gccgaagatg aacgcatcat
     5521 tggtatctgt tccgaaaatg ttaccatcgc cacctacacc tttcaggaag taaccgatga
     5581 taccgaacac gaacatcgga aggatggttg ctgccaggat cacaccggtt cccatatcgc
     5641 cgggtttgac cggtttcagt gtatcagctt ccttcacgtg tctggtacca ctggcaaaca
     5701 cgatcagacc gatgatcata ccgataccgg cagcaatgaa ggcgaagccc cagccgtagt
     5761 tgttgcgcag aaaagcagcc acgaagttgc aaatgaaggc accgatattg atgcccatgt
     5821 aaaagatgtt gtacccggaa tctttattgg ctatgtagcg tggatcattg tatagattac
     5881 ctaccagggt agaaatgttt ggtttgaaga aaccgttgcc caggatgatg agcagcaacg
     5941 atacatagaa tgctgtctcg ttgtgaatgg ataatcccag gtatccggca gccatgagag
     6001 agcctcccag aaagatggac ctcctgtacc cgaggatcct gtcggccagc agaccaccta
     6061 taaaaggggt gagataaacc agtcctatat aggtaccgta gatgtcagat tttttagcac
     6121 ctgaaaatcc catacctccg ttctccaggc tgtccagcat gtacagggag aagataccca
     6181 gcatgaggta gtagccgaag cgctcccaca tttccgtaaa gaacagcaca tacaatcctt
     6241 tcgggtgtgc tgccagtttg cgttctgcag tcatgtaatg ttttttattg gttgactaaa
     6301 aatatcattt ctccggaaat caggccgttg ccatctggtc atcagatggg atcttagcga
     6361 tccgtatgcc cagatagcca tataatatgg tcataagcgg actgatgata caaaagaaac
     6421 agaatggcag ataggtcagg gtggccacac ccaatacacc ggcctgcgta gcaccacagg
     6481 tattccaggg taccagaacc gaggtgacgg tgccgctgtc ttccagggta cggctgaggt
     6541 tttgcggggc cagtccacgc tggcggaagg tcttggcaaa cattcttccg ggtactacaa
     6601 tggccaggta ctgatcagag gcggtcacat tcaggaatac gcaggaggta gctgtagtgg
     6661 caatgaggga acctgtggtc tttgcacgtt ccacgagtgg tcgtgtgata cgagccagca
     6721 aacccacact ttccatgacc cctccgaaac acatagcaca tacgatcagc cagatggtat
     6781 tcagcattcc tgccataccg cgggcactca gcagaccggc agcgatctca ttatcgcttg
     6841 atacggaaat ggaccccgac atggcattta ccactgccat ataggattgt ttggcatagg
     6901 aaccggtgat gccggaaacc tccgccacga cctccggctg gaagatgatg gccaccactc
     6961 ctcccaacag tgatcctatg agcaaagcgg tgatagggtc tgctttctta atgatcatcc
     7021 atatgacaac ggccggtacg atgaagagaa aaatattgat gtgaaaggat tggtggatgg
     7081 cattgcccag ggcattcatg ttctctggcg acacttcagg catatgaaag aaacctataa
     7141 aaagaaataa caacaatgta atgaccatac ttggcactgt ggtcagtacc atgtagcgga
     7201 tatgtgtgaa caggtcggta cccgccatag ccggggccag gttggtggta tcactcatgg
     7261 gcgacatctt atcaccgaag taagcaccac tgatgatggc tcctgctatc catccctcgt
     7321 tcactcccat agcctgtccg ataccgagca gggcgatgcc taccgtggct accgtactcc
     7381 aggaagaacc tgttgccaat gaaaccactg cgcagataat acaggctgcg aagagaaaga
     7441 ttgtgggatt cagaatctgt aatccatagt agatcatggc aggtaccaca ccgctgataa
     7501 gccaggttcc tgccagtgcg ccgatgagca gcaggatcag gatggcattc atggcagaca
     7561 tgatgctgtg taccacccct tcgatcatgt gggcaagcgg aaccttcagc atcagaccca
     7621 ttacagcggc taccgcagca gcaatgatca gtgctatctg attggcacct gatgtggcca
     7681 tgtctccaaa gaagagcacg ttcaacacca gtaatcctac cagcaccagt accggcagca
     7741 aggcatgtaa tattcccggt tcctgtccgt cagggtgtaa acgatcgtcc ataaacgctg
     7801 tctgattggt tgcaccaaaa tacaggtttc cgtccataca gaccctgtgt tctgttatac
     7861 cggaatagcc ggacagtttc tgatgctact tacaacggta ataaaaggcc acaaagctgc
     7921 ggccttttat tatcttgatt cacctgccgg tcgttgtacc ggcgaaccta ttgtgctctc
     7981 cgtggcgggg atagcctgag gatagctgag ggctcttcct ttgtcaggga gacaatgtcc
     8041 tgccagacac ctccctgcgc tctttggtga tctcatcata cctctccata tccaggagca
     8101 ggtgggcata gtggttgttg taggatgcca gtttgaattc ctgggttccg tacatcacat
     8161 atccgtatac accattttta tcgtaagcca tcacggtata gcgaccgccg gcaccctggt
     8221 tcgggccgaa ccggaggtat tctccgttgg cgtcgaaact gacatgcagg atattacctg
     8281 cgatacctgt a
//
LOCUS       DAOMAX010000106         8280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1270_length_8280_cov_4.067842, whole genome shotgun sequence.
ACCESSION   DAOMAX010000106 DAOMAX010000000
VERSION     DAOMAX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_8280_cov_4.067842"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="pepE"
                     /locus_tag="PK742_06935"
     CDS             <1..523
                     /gene="pepE"
                     /locus_tag="PK742_06935"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF003642.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HQU76361.1"
                     /translation="GMNLRGLETFADPVAAIREAKGVFTGGGNTFVLLKELYDSGVLD
                     VLRHKILDGMPYMGTSAGSNITGMTIGTTNDMPIVYPPSFEALGVLPVNINPHYLDPD
                     PDSKHMGETRETRIFEFHTMNNQPVIGLREGSWLEIRNEKALLKGALPARLFSQGKDP
                     EELSPNTDISFLL"
     gene            520..1446
                     /locus_tag="PK742_06940"
     CDS             520..1446
                     /locus_tag="PK742_06940"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQU76362.1"
                     /translation="MNNKAFPVWQVAVLLLAAVLIIPALVVSVQQPLAPLQLRMLRES
                     GMAMAALAVLCFMVSSLTGNHSQVDKLWSIVPMGYVWYFAWMNNMEDRSVLMAVMVTI
                     WGFRLTYNFARRGGYSWKFWTGEEDYRWSLLRKSPAFNATWRWVLFNLLFISLYQMTL
                     IWLFCLPAVAVAGMDTKLTMVDWLLATAMFLAILLETIADQQQWNFQRKKLSQANTVE
                     TAKGFLDTGLWSYVRHPNYTAEQSVWILFYGFSIVATEQWINWSVIGCILLLMLFQGS
                     ARFSEGISAGKYPDYHQYMQQTSRFIPLRIKR"
     gene            1572..2924
                     /locus_tag="PK742_06945"
     CDS             1572..2924
                     /locus_tag="PK742_06945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HQU76363.1"
                     /translation="MKISLKQLSLATAVSAVMLTVGCAKEADFLQDQTGLDLEAKVST
                     SDFDATVPQAYMDHMLTMVKATPGWTPPVAARGFGYTGLALYESVVPGISGGNSMVGQ
                     LNELTSLPEIEADEKYQWEICANEALYGIISSLMPTASADNLAILASIHDDFDAGFAS
                     VPADIYERSVAFGQAIAEAIYDYSTTDGGHECYLYNFPADYVPPVGDGMWIPTPPGYS
                     AALQPYWGDVRPFIAADVVEAIAVPPYAYSETAGSTMYNQALETFNYVADATDETTDI
                     ALFWADDPGATFTPPGHAWHIAMQVISDEDVSLAQAALTYGKLGVALNDAFITCWKNK
                     YIYNLIRPISYIQEMWDPSFATIVGTPPFPEYTSGHSTNMGAWKKVMEDIYGKSYAFT
                     DHTHEAVYGTRSFANFNEAAYEAAISRMYGGIHYKQACYQGVKMGEIVGENVNDLNWY
                     "
     gene            2997..3716
                     /locus_tag="PK742_06950"
     CDS             2997..3716
                     /locus_tag="PK742_06950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQU76364.1"
                     /translation="MKKIKLVLQIAAVLILQACSKNGQTPVNQAETEIVAGNALDSVS
                     YAIGQNVARILKGQGFDTLNYSVFTAAIESSMNGEQLWFDPAEANRYINLYVIDQVRE
                     LSSRNLAFANTWLDSIAHLQGVREVSKGVYMRITKEGNGKQPAATDEVEINFHGELPD
                     GEVFDDSFARNSSVTFVVNSAIDGWQAVLPLLQEGAEAEIWLHPDMGYGTQGGYFGVI
                     PANSALYYKMEFIRIVPGGGY"
     gene            complement(3713..5191)
                     /gene="proS"
                     /locus_tag="PK742_06955"
     CDS             complement(3713..5191)
                     /gene="proS"
                     /locus_tag="PK742_06955"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQU76365.1"
                     /translation="MAEKITSREVDYSQWYNDLVLQSGLAEYSPVRGSMVIKPYGWAI
                     WEEMKSILDKRFKETGHVNASFPLLIPKGFLEQEEGHAEGFAKECAVVTHYRLKTDPD
                     KKGKLMVDPESRLEEELIIRPTSETIIWNCYKNWIRSYRDLPILINQWANVMRWEMRT
                     RLFLRTSEFHWQEGHTAHATKEEAIAETEQMLHIYAEFMEQYMAIPVWRGIKTASERF
                     AGAEETYCVEGLMQDGKALQMGTSHFLGQNFAKAFDVTFLNRENKEEYAWATSWGVST
                     RMMGALIMAHSDDNGLVIPPRLAPLHVVIIPIYRNDEQLDQIRAKVDPLMTELKQLGI
                     ICKFDDSDQNKPGWKYAEYELKGVPVRLAMGMRDLENGTVEVARRDTREKMVWEMTGL
                     SDRIRQLLDEIQQQIFRNALAFREDNTHTVNTWDEFREIINSKGGFILAHWDGTAETE
                     EQIKEATKATIRCIPLDAEEEDGVCVFSGKPSKRRVVFAKAY"
     gene            5285..6667
                     /locus_tag="PK742_06960"
     CDS             5285..6667
                     /locus_tag="PK742_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76366.1"
                     /translation="MKLFTILPVSLLATGTFLLSGTMSAQVYDDLYYTPGSSTSNTTT
                     ETYTTPDYGSDYGTSDSDYRVYSDDFSSEKYYDEDGNTYVVNNYYYEDDWDDYSYSMY
                     VNRFYTPYYGFSYYSPCYDPWFWRPGWGWNVGYNSWTGWNVGWGYSWGWCSGWGWGYG
                     NPWYNPYYSPWYSPYAYYGWGYSPWYNPYHYGWGNAYYNGYNNGYWNGYANGYYDGYY
                     NGYGNGYYYGPREATASNTGDRDYTSGVMGTDRDNGAIFSEAPGRGKSLDNTGDHVSA
                     AQDRGLNSITRDVHTSAAQPVEGRDQTNLSSVKVVKHYSAQEAAESPVRGDVISRPDV
                     RQERSDVLNNSTQPKGYESRPSDQYQWRDNSQPRNQERPAQRYESTTPDRSKSQPNYS
                     QPRNSQSQPKYNNNSNRNNTPSQPKYNNSSSSSNRNSGWNNSGSSSRSSSGTRSSGSR
                     SSSSTNRQPR"
     gene            6700..8211
                     /locus_tag="PK742_06965"
     CDS             6700..8211
                     /locus_tag="PK742_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76367.1"
                     /translation="MKKQYAILALALAMPILSQAQSDEDALRYSMLGFGGTARSLGVA
                     NAFGALGGDFSSLAMNPAGIGIYRSSEFVFTPGLTHFDNQSAFYGSATNADKYNFNLS
                     NLGMVFTNMKTGKANADNGWVATQFAVGYNRLENFNNRLIYTGFNDRNSLTSLYVEQL
                     NANGGTNPGSVTGSFPFGAGLAWETYLINPNFNDSTSYYTEIQGGNIQQTKTLETSGA
                     YDELDFSFGGNYGNKLYVGFTLGVPIVDYNAVVTYTEEDVQNVHDNFNQMTLTDQLNV
                     NGAGINGKFGVIYRVNDYLRLGGAVHTPTALGLTDVYRSTMTSSLDTSGSYGYDSPTG
                     RYEYSIVTPWRAIASAAITMKQYGFISFDYEFVDYSAAGFNFNRIGSVDDITLETALN
                     NTINEKYTGAHNIRVGAELAYEVFRFRGGYAIMGTPFASGFAEGSADFARSTYTLGAG
                     IRDEHFYVDLGYANTRSTEYDTQYNFSDGNGANEGATIDKTVSNFLLSLGFRF"
BASE COUNT         2104 a         2001 c         2023 g         2152 t
ORIGIN      
        1 cggaatgaat ctgcgtgggc tggaaacctt tgctgatcct gtggccgcta tccgcgaggc
       61 gaaaggggtg tttaccggtg gaggcaatac ttttgtatta ttgaaggaac tatatgattc
      121 aggagtcctg gatgtgctca gacacaaaat actggatggc atgccttaca tgggtacaag
      181 cgcaggtagc aatataacgg gaatgaccat tggtaccacc aatgacatgc ccattgtcta
      241 tcctccttca tttgaggccc tgggggtgtt acctgttaat attaaccccc actacctgga
      301 ccccgaccca gacagcaagc atatgggtga aacaagagaa accaggatct ttgagtttca
      361 taccatgaat aaccaaccgg tcatcggttt gcgtgaaggc agctggcttg agatcagaaa
      421 tgaaaaagct ctgctgaaag gggcactgcc ggcccgctta ttcagtcagg gaaaagatcc
      481 tgaagaatta tctcccaaca cagatatcag ttttctgtta tgaataataa ggcatttccg
      541 gtatggcagg tagcagtgct tctgctggct gctgtcctga tcattccggc gctggtggtt
      601 tccgtgcagc aacctctggc accattgcaa cttcgcatgt tacgcgaaag cggtatggcc
      661 atggcagcac tggctgtatt gtgtttcatg gtgtcttctc tgactggaaa ccacagtcag
      721 gttgataaat tatggagcat cgtaccaatg ggttacgttt ggtattttgc ctggatgaac
      781 aacatggaag acaggtctgt actgatggca gtgatggtca cgatatgggg ttttcggctg
      841 acctataatt ttgccagacg aggtggttac agctggaaat tctggaccgg cgaagaagac
      901 taccggtggt cattactcag gaaaagcccg gcattcaatg ccacctggag gtgggtgctt
      961 ttcaacctgt tgttcatcag tctgtatcag atgaccctca tctggttgtt ctgtcttccg
     1021 gctgtagcag ttgcaggaat ggatacgaaa cttacaatgg ttgactggct gttggcaact
     1081 gcaatgtttc ttgcaattct gctggagacc atcgctgatc agcagcaatg gaattttcag
     1141 cgaaagaaac tcagtcaggc caatactgtt gagacggcca aaggtttcct ggatacggga
     1201 ttatggtcat atgtgcggca tcctaattat acagctgagc aaagtgtatg gattttattt
     1261 tacggtttta gtattgtggc cacagagcaa tggatcaact ggtctgtgat aggctgtatc
     1321 ctgttactga tgctgttcca ggggtctgca cgcttcagtg aaggaatatc tgcaggcaag
     1381 tatcctgatt atcatcaata tatgcagcaa acaagccgct ttattccgct ccggatcaag
     1441 cgttgatacg ggtggtttga aaataattta aatatcgttt catttagttt acaaagcttg
     1501 ctgatctatt tacctggagt cttttgagtt ggatgctgta cttattccct ttgcagcaaa
     1561 caactctgga tatgaaaatt tccctgaaac aattatccct tgccacagca gtttcggctg
     1621 taatgctaac tgtcgggtgt gccaaggaag cagactttct gcaagatcag actggtctgg
     1681 acctggaagc taaagtgtca acctcagatt ttgatgccac tgtgcctcag gcttacatgg
     1741 atcatatgct gaccatggta aaagccacac cgggctggac ccctcctgtt gctgcaagag
     1801 gttttggtta caccggtctg gcattgtatg aatctgttgt acccggaata tctggcggta
     1861 acagcatggt agggcagttg aatgaactga cctctttacc ggagatcgaa gctgatgaaa
     1921 aatatcagtg ggagatctgt gccaatgaag cgctgtacgg catcatcagc agtttgatgc
     1981 caactgcatc ggcagataac ctggcaatac tggcttctat ccacgatgat ttcgacgccg
     2041 gttttgcttc tgtacctgca gatatctatg agcgctcagt ggctttcggt caggccatcg
     2101 ctgaggccat ctatgattat tccaccacag atggaggtca cgaatgctat ctctacaact
     2161 tccctgcaga ctatgttccg cctgtaggtg atggtatgtg gattcctact cctccgggct
     2221 actctgcagc acttcagcca tactggggtg atgtgcgccc gttcatcgca gccgatgtgg
     2281 tagaagctat tgccgtacct ccctatgcgt attctgaaac agctggttct actatgtaca
     2341 accaggccct ggaaactttc aactacgtgg ctgatgctac agatgaaaca acagatatcg
     2401 ccctgttctg ggctgatgat ccgggtgcca cctttacccc tccaggccac gcatggcata
     2461 ttgctatgca ggtgatcagc gatgaggatg tcagccttgc tcaggcagca cttacctatg
     2521 gtaaactggg tgtagccctg aatgacgcct ttattacatg ctggaaaaat aaatacatat
     2581 acaacctgat cagacccatc agttatatac aggaaatgtg ggatccttct tttgctacta
     2641 ttgtaggtac tccgcctttc cctgaatata cttccggcca cagtaccaac atgggtgcct
     2701 ggaaaaaggt gatggaagat atttatggca agtcatatgc ttttacagat catacacacg
     2761 aagcagtgta cggaaccaga agctttgcca atttcaatga ggcagcctat gaagcagcta
     2821 tcagccgcat gtatggagga atccactaca agcaagcctg ttaccagggt gtcaaaatgg
     2881 gtgaaattgt cggagagaat gtcaatgacc tgaactggta ttgatcgcgt tctcttctga
     2941 tgtggatata aggatgggtc gccctgcggc ctgtccttat tacttataca acgactatga
     3001 agaaaatcaa acttgtacta cagattgctg ctgtcttaat tcttcaagcc tgcagcaaga
     3061 acggccaaac gccggtcaat caggctgaaa cggaaatagt tgccggtaat gccctggatt
     3121 ctgtaagcta tgccattggg cagaatgtgg ccagaatcct gaagggtcag ggttttgata
     3181 cgcttaatta ctctgtcttt actgcggcca ttgaaagctc catgaacggt gagcaactct
     3241 ggttcgatcc ggcagaagcc aatcgctata tcaatttgta tgtgatcgat caggtcagag
     3301 aactctcttc caggaacctg gcatttgcca atacctggtt ggattccatt gcgcatctgc
     3361 aaggtgtcag agaggtaagc aaaggtgtgt acatgcgcat caccaaagaa ggtaatggta
     3421 aacaaccggc tgcaacagat gaagtagaga tcaactttca tggtgagctg cctgacggag
     3481 aggtctttga tgacagtttc gctaggaatt cttccgtgac ttttgtggtc aattctgcca
     3541 tagatggctg gcaggcagta ttgccactat tgcaggaggg tgctgaggca gagatctggc
     3601 tgcatccaga tatgggctat ggcacacagg gcggttactt tggcgtgatc ccggccaaca
     3661 gtgccctgta ttataagatg gaattcatcc ggatcgtgcc cggaggaggt tattaataag
     3721 ctttggcaaa aactaccctg cgcttgctgg gttttccgga gaagacgcac acaccatctt
     3781 cttcttcggc gtccagcgga atgcaacgaa tggtagcttt ggtagcttcc ttgatctgct
     3841 cttcagtttc tgcagtgcca tcccagtgag ccagaataaa gcctcccttg ctgttaatga
     3901 tctctctgaa ttcatcccag gtgttcacag tatgggtgtt gtcttctctg aatgccagtg
     3961 cattccggaa tatctgttgc tggatctcat ccagtaactg acgtatcctg tctgaaagac
     4021 cggtcatttc ccagaccatc ttttcacggg tatcacgccg ggccacttct accgtgccgt
     4081 tttccagatc acgcattccc atggccagtc ttacaggtac tcccttgagt tcgtattctg
     4141 catatttcca gcccggtttg ttctggtcgc tgtcatcaaa tttgcaaata atgcccaatt
     4201 gcttcagctc tgtcatcaaa gggtccacct tcgcccggat ctgatcaagt tgttcatcat
     4261 tgcggtagat cggaatgatg accacatgca aaggagccag ccgtggagga atgaccagcc
     4321 cgttgtcatc gctgtgtgcc atgatcaacg cacccatcat tcgggtactc actccccagg
     4381 atgttgccca ggcatattcc tctttgtttt cgcggttcag aaaggtcaca tcaaaggctt
     4441 tggcgaagtt ctgccccaga aaatgactgg ttcccatctg cagcgccttg ccatcctgca
     4501 tcagcccttc tacacaatag gtctcttcgg cacccgcaaa tcgctcactg gctgtcttga
     4561 ttcccctcca gaccggaatg gccatgtact gctccatgaa ttctgcatat atatgcagca
     4621 tctgttcggt ctcagcaatg gcctcttctt tggttgcgtg ggcggtatgc ccttcctgcc
     4681 agtgaaactc tgaggtgcgc agaaacaacc tggtgcgcat ttcccagcgc atgacattgg
     4741 cccactggtt gatcaggatg ggaagatctc ggtagctgcg tatccagttc ttatagcaat
     4801 tccatataat ggtctcggaa gtaggccgaa tgatgagctc ttcctccagc ctggattcgg
     4861 ggtccaccat gagcttccct tttttgtcag ggtcggtttt taaccgatag tgggtcacta
     4921 cggcacattc tttggcaaaa ccttcagcat gcccttcttc ctgctccaga aagcccttgg
     4981 gaatcagcag cggaaaactg gcattcacat gtcctgtctc tttgaaacgc ttgtccagaa
     5041 tactcttcat ctcttcccag atggcccagc catagggttt gatgaccatg cttccccgca
     5101 caggagagta ctctgccagc ccgctctgca gcaccaggtc attgtaccac tggctgtaat
     5161 ctacctccct gcttgtgatc ttttcggcca tttcttaagt ttggtatgat gtttgaatct
     5221 aattctgtgt gcaaaaataa taaagctgca caggacgatt aggaaatcag ctaaatcagg
     5281 tcttatgaaa ctctttacaa tactccccgt ctcactgctc gcaacaggca cattcctgtt
     5341 gtccgggact atgtccgccc aggtctatga tgacctctat tatacaccag gcagcagtac
     5401 cagcaacacc accacagaga cctataccac accggattac ggttctgatt acggaaccag
     5461 tgattcggat taccgcgtct attctgacga cttttcctct gaaaagtact acgatgaaga
     5521 cggcaacacc tatgtggtaa acaattatta ctacgaagat gattgggatg attacagcta
     5581 ttccatgtac gtaaatcgtt tctacacccc gtattacggt ttcagctact attctccctg
     5641 ttatgatcct tggttctggc gacccggttg gggatggaac gttggctata acagctggac
     5701 cggctggaat gtaggctggg gttattcctg gggatggtgc tccggttggg gatggggtta
     5761 tggaaaccca tggtacaatc catattacag cccatggtac agtccttatg cctactatgg
     5821 atggggttac agcccctggt ataatccgta tcactatggt tggggaaatg cctattacaa
     5881 tggctataac aacggttact ggaatggcta tgccaacgga tactacgatg gttactacaa
     5941 cggctacggc aacggctact attacggacc tcgtgaggct actgcttcca ataccggaga
     6001 tcgtgactac acttctggtg tgatgggtac tgaccgcgac aacggcgcca tcttctctga
     6061 agctcccgga cgtggcaaga gtctggacaa cacaggtgat cacgtttctg cagcacagga
     6121 ccgcggactg aacagtatta caagagatgt acataccagc gctgcacagc cggttgaagg
     6181 aagagatcag accaacctgt catctgtgaa ggtggtgaaa cactacagtg cccaggaagc
     6241 agcagagtct cccgttcgcg gtgatgtgat ctcacgcccc gatgtcaggc aagaaaggtc
     6301 tgatgtgctg aacaacagca cccagcctaa aggttatgaa agcaggcctt ctgatcagta
     6361 tcagtggaga gataacagcc agcccagaaa ccaggaaaga cctgctcagc gctatgagtc
     6421 cactactcct gatcgttcca agagccagcc gaattatagc cagcccagga acagccagag
     6481 tcagccgaag tataacaata actccaacag gaacaatacg cccagtcagc cgaagtataa
     6541 taacagcagc agcagcagca acaggaacag cggatggaac aactctggca gcagtagcag
     6601 aagttcatca ggaacccgca gctccggttc acgcagcagc agctcaacca acaggcagcc
     6661 caggtaagca gcgtctgatc acacagaaaa cggaacaaca tgaaaaagca atacgcaata
     6721 cttgctctgg cccttgccat gcccatactg tcacaggcac agagcgatga agatgccctc
     6781 cggtactcta tgctgggttt cggcggaacc gcaaggtctc ttggtgtagc caatgccttt
     6841 ggtgcccttg gcggagattt cagctcactg gccatgaacc ccgcagggat cggtatctac
     6901 agaagttcag aatttgtatt tactcccggg ctgacgcatt tcgataacca gagtgcattc
     6961 tacggaagcg ctaccaatgc agataaatac aatttcaatc tgtctaatct gggtatggtc
     7021 tttaccaata tgaaaacagg aaaggccaat gcagataacg gatgggtggc tacccagttt
     7081 gcagtagggt acaacaggct ggagaacttc aacaacagac ttatttacac cggctttaac
     7141 gatcgcaact cacttaccag cctgtatgtg gagcagctca atgccaacgg aggtaccaat
     7201 ccgggcagtg tgaccggttc cttccctttt ggcgcaggcc ttgcctggga aacctatctc
     7261 atcaacccga acttcaacga cagtaccagt tattataccg aaatacaggg ggggaatatc
     7321 caacagacaa aaaccctgga aacatccggt gcctatgatg aactggactt ttcctttggc
     7381 ggcaactatg gcaataaact gtatgtgggc tttaccctgg gtgtgccgat agttgattac
     7441 aatgcagtgg ttacctatac ggaggaagat gtacagaatg tacacgacaa cttcaaccaa
     7501 atgacactca ctgatcagct gaatgtaaac ggagccggta tcaatggaaa atttggggtg
     7561 atctaccggg taaatgacta cctgagattg ggcggagcag tgcatacacc aactgccctg
     7621 gggctgaccg atgtgtacag aagtaccatg acctccagtc tggatacttc cggatcatat
     7681 ggctatgatt cgccaaccgg tcgctatgaa tacagcatcg tcactccgtg gagagccatt
     7741 gcatcagctg caattactat gaaacagtac ggtttcatca gttttgacta tgaatttgtg
     7801 gactacagcg cagcaggttt caacttcaac cggatcggtt cggtagatga tattacactc
     7861 gagaccgcac tcaataatac catcaatgaa aaatataccg gtgcacataa catccgtgtg
     7921 ggtgcagaac tggcatacga ggtattccgt ttcagaggtg gttatgccat tatgggaaca
     7981 cctttcgcaa gcggatttgc agaaggcagt gcagattttg cccgaagtac ctataccctt
     8041 ggtgctggta tccgcgatga acatttttat gtggatctcg gttacgctaa tacacgttca
     8101 acagagtatg atacacaata taatttcagc gacggaaatg gcgccaatga aggagcaacc
     8161 attgacaaaa ccgtcagcaa ttttcttttg agtcttggtt ttaggtttta atgattgtca
     8221 gtaaggtggc cggcggcccg cagcaatgcg ggccgccggt gttttgtacc aaccttcttt
//
LOCUS       DAOMAX010000107         8212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1279_length_8212_cov_3.378080, whole genome shotgun sequence.
ACCESSION   DAOMAX010000107 DAOMAX010000000
VERSION     DAOMAX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_8212_cov_3.378080"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK742_06970"
     CDS             complement(<1..438)
                     /locus_tag="PK742_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQU76368.1"
                     /translation="MELEKFSYDNQIVRKFLIATVVWGIVGMTVGLWVALQLVYPQLN
                     LGIDFTTYGRIRPVHTNAVIFAFVGNGIFMGVYYSLQRLCKTPMFSKMLSNIHFWGWQ
                     LIIVSAALTLPLGYTTGKEYAELEWPIDIMIALIWVVFGINMFG"
     gene            complement(441..617)
                     /gene="ccoS"
                     /locus_tag="PK742_06975"
     CDS             complement(441..617)
                     /gene="ccoS"
                     /locus_tag="PK742_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQU76369.1"
                     /translation="MSVIFLLIGCSLLLAIIFLFIFIRSAKRGQFDDDYTPSVRILFD
                     DVPPRKSNHLKSKH"
     gene            complement(703..3102)
                     /locus_tag="PK742_06980"
     CDS             complement(703..3102)
                     /locus_tag="PK742_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HQU76370.1"
                     /translation="MARQGETTTRLTCYHCGEDCTEEVYYVDEKPFCCFGCKTVYGIL
                     ADNDLCAYYELESAPGKNRRDAVETSKYEILDRPDIRARFVTFEDARQMHMRFYLPHM
                     HCSSCIWLLENLHTLHPAVAQARVDFRRKEITIIAAREEISVREVASLLDTIGYEPYL
                     SLQDMEKKDDGSVNRGRLIRMAVAGFCFGNIMLFSAPEYLAGGAMGETSLRLLFQYLS
                     LLFSLPVFFYCADEFYISSWKSLRKGFLHIDVPIALAIIITFVRSLYTVFVEGSGGYF
                     DSMSGIVFFMLLGRYFQDRTYKMVSFTRDYKSYFPLSATKLEDGKEEAVAVTELQKGD
                     TYIVRNNELIPADSILIRGQARIDYSFVTGEAEPVEKSIGELIYAGGRQRGSHLELRV
                     IKEVSQSYLTQLWNQSGNRQKQDEESRTMVQLLSKHFSLIVLILSVMAFIFWLFMDPS
                     RSLDALVTPMIIACPCALLLSATFTNGNAIARLGKAGLYLRNAGVIEKLTRVSVIVFD
                     KTGTITPSRHTRIEYEGTPLQSEERSLLSSLVHESSHPYSRAIATWCGTGKLQPVRHF
                     REIAGSGIEGEIGHTKVRLMRAPDERQGTVFLVNGEELGMFRFSAEVYPGMFALTGRL
                     RREYPIYLLSGDSDRDKPVMDQLFDGNHQRYYCQPEDKRRFIEELQKSGEVVMMVGDG
                     LNDAGALMQSDVGVAVTDDIHSFTPASDLIMEVQGIGQTDRLLRYAAAARKVIIWSFV
                     ISLIYNVAGLWFALQGLLQPVIAAILMPLSTITIVLFTTGLTSLFSRRLLRPVAGSDP
                     E"
     gene            3221..3832
                     /locus_tag="PK742_06985"
     CDS             3221..3832
                     /locus_tag="PK742_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76371.1"
                     /translation="MRRILPLVMLFIVSVAGNHVLAQLTEIDPKDDNEPRQTESVEKD
                     KDKKDSRFVYGGFMAAGFGNYTTINLSPVVGYRVTDKFTPGIGFTWQYAQYKDADNMV
                     YNPFADYKFNTLGGRVFMQYSILYGVFAHAEYEQLWYNFEFLESPYLQINDQQPGLFL
                     GGGYRFGAGNSAGLQILALWNALWDTDNLIYPNPWTLRFSVMF"
     gene            complement(3833..5773)
                     /gene="gyrB"
                     /locus_tag="PK742_06990"
     CDS             complement(3833..5773)
                     /gene="gyrB"
                     /locus_tag="PK742_06990"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQU76372.1"
                     /translation="MNAPKSNNYTADNIQVLEGLEAVRKRPAMYIGDTGVKGLHHLVY
                     EVVDNSIDEALAGYASTVLVTIHTDNSITVQDDGRGIPTEIHQKEKKSALEVVMTVLH
                     AGGKFDKDTYKVSGGLHGVGVSCVNALSTHLKAEVHRNGKIYIQEYSIGKPLYAVKEA
                     GETDRTGTMVTFRPDDTIFTVTEYKFDTLAARLRELAFLNKGIKLSLTDEREKQEDGS
                     FLSKEFFSEDGLTGFVRYLDGSRSSIIPEPIYTEGARDGVQVELALQYNDSYNENIHS
                     YVNNINTIEGGTHVSGFRRALTRTFKAYGDREGLFEKAKVDITGDDFREGLTALVSVK
                     VPEPQFEGQTKTKLGNSEVLGIVDTIAGEALNNYLEENPKMARMIIGKVVLAAQARTA
                     ARKARELVQRKGALSSTGLPGKLADCAEKDPEKCELFLVEGDSAGGTAKQGRDRHTQA
                     ILPLRGKILNVEKALDYKVFDNEEIKNIFTALGVMPNEDKSIDTTKLRYHKIVIMTDA
                     DVDGSHITTLILTFFFRKMYELVELGYIYIATPPLYMVKKGKDFKYCWNEEQRKEAVT
                     ELAKGGKEESVNIQRYKGLGEMNAEQLWETTMNPETRTLRRVTIESAAEADRIFSMLM
                     GDEVPPRREFIEANARYAKLDV"
     gene            complement(5913..7577)
                     /locus_tag="PK742_06995"
     CDS             complement(5913..7577)
                     /locus_tag="PK742_06995"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQU76373.1"
                     /translation="MKWWSTGVLLLVMMHAQAQLFDNKGSFTHADTIRGSITPERSWW
                     NVTHYDLTIRFNEENKTLEGKNTISFRVEDTGSSMQIDLMQPLEVDRVLLDGEPVSIS
                     RHDDVFYLQTPSLANGSIHKLEISYHGKPRVARLPPWDGGVIWSRDHNGNSWISVACQ
                     GLGASVWYPNKDHQSDEPDSAAMHIITNKGQTAVSNGRFRGMTENADGSLTWNWAVVN
                     PINNYNLIPYIGKYEDHHSTYQGEAGTLDVDMWFLEGHMDQAISHVLPDINKTLEAFE
                     YWFGPYPFYEDGFKMVEAPHLGMEHQSAIAYGNKFKKGYLGGDLSGTGEGDDWDYIVV
                     HESGHEWFGNNITTKDIADMWVHEGFTDYSETLFIEYWKGKDAADKYVRGLRKNISND
                     EPVIGPYLVNTEGSGDMYYKGANLLHTIRQVIDNDSLFRDILRGLNHDFYHQTVTSLM
                     VEDYISRKAGMDLGRIFTQYLRTTHIPVFDYHFEDGKSGPVLVYRWANIVKGMNMPYD
                     IQVDDQLVRLYPSQEYQYYDLPAGFDTRKNISGNLDYYVLTQRSGK"
     gene            complement(7649..>8212)
                     /locus_tag="PK742_07000"
     CDS             complement(7649..>8212)
                     /locus_tag="PK742_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76374.1"
                     /translation="AGDLNLIAGYQAMTLRSGRATHTLFVQAGAELPTGKYKADFVQA
                     GLPPGFYPGSASIDPLAGIQYILSDSAWTAMFGASVKYSLPNADHLQEGLFWQAQTVV
                     ARSLGASGRWVPFAGLVAEHRNGYRKYDFLNEESAYSVAHGMAGMEYRGDRFSVGARF
                     DHTIWQQYSTEETTNNGAYSVYVNWFF"
BASE COUNT         1964 a         2136 c         1934 g         2178 t
ORIGIN      
        1 tccgaacata ttgataccaa acaccaccca gatcagggcg atcatgatat caattggcca
       61 ttccagttca gcgtattctt tacctgtagt gtaacccagt ggcaaagtaa gtgcagcaga
      121 cacgatgatc agctgccagc cccagaagtg aatattgctc agcattttac tgaacatggg
      181 tgttttacat aaacgctgca gggaatagta aacccccatg aagatgccat tacccacaaa
      241 tgcgaagatg accgcattgg tgtgtacagg tctgatcctg ccataggtgg taaaatcaat
      301 tcccaggttg agttgcggat acaccagctg cagtgcaacc cacaaaccta ccgtcatgcc
      361 cacgattccc catacgacgg tcgcgatgag gaatttgcgg acgatctggt tgtcatagct
      421 gaatttttcg agctccatac tcagtgcttg gatttaaggt gattggattt tctgggcgga
      481 acatcgtcaa agaggatacg cacagaaggt gtgtaatcgt catcaaactg cccgcgtttg
      541 gcggatcgaa tgaagatgaa cagaaagatg atagccagca gcaggctgca tccgatcaac
      601 agaaagatga cgctcataat cggacactaa attccaacat ttggacagtc agctgtgttg
      661 atgctactca gagcggttgg tgacttttat caacctccgg cctcattctg ggtccgaacc
      721 agcgacaggt cgcagcaaac gccgcgagaa cagagaggtt agcccggtgg tgaataaaac
      781 gatggtaata gtgctgaggg gcatgagaat agcggcaatg accggctgca ataacccctg
      841 cagagcgaac cagaggcctg ccacgttata gatcagagag atcacaaagc tccagataat
      901 gacctttctg gcagcagcgg cataccggag caggcggtcg gtctgaccga tgccctgtac
      961 ttccatgatg agatcgctgg caggtgtgaa gctgtgtata tcgtcggtca cggcaactcc
     1021 tacatcactt tgcatcagtg caccggcgtc attgaggcca tcacccacca tcatcaccac
     1081 ttcacctgac ttttgcagtt cttcaatgaa acggcgcttg tcttccggct ggcaataata
     1141 tcgctggtgg ttgccatcaa acaactgatc catcaccggc ttatcgcgat cactgtcgcc
     1201 tgacagcaga tatattgggt attctcttcg caatctgcct gtcaatgcaa acattccggg
     1261 gtacacctca gcactgaagc ggaacatccc cagttcttct ccgttcacca ggaataccgt
     1321 accctgtcgt tcatccggag cccgcatcag tcgtaccttt gtatgaccga tctctccttc
     1381 aattcctgaa ccagctattt ctctgaaatg gcgcacgggc tgaagctttc cggttccgca
     1441 ccacgttgca atggcacggc tgtaaggatg ggaagattcg tgcaccaggc tggaaagcag
     1501 tgatcgttct tcactttgca gcggagttcc ttcgtattct atcctggtat gccggctggg
     1561 ggtgatggtt ccggtcttat caaaaactat gaccgatacc cgggtcagtt tttctatgac
     1621 acctgcgttg cgcagataca ggcccgcttt acccagcctg gctatggcat tgccgttggt
     1681 gaaagtggca gacagcagca gtgcacaagg gcaggctatg atcatgggtg taacgagtgc
     1741 atcgagtgat ctggacggat ccatgaataa ccagaatatg aaagccatga ccgacaggat
     1801 cagcacaatg aggctaaaat gcttgctgag taactgcacc atcgtcctgc tctcttcatc
     1861 ctgtttctgc ctgttgccag actggttcca caactgagtc agatagcttt gtgaaacctc
     1921 tttgatgacc cgaagctcca gatggcttcc gcgttgtctt cccccagcgt agatcagctc
     1981 accaatactc ttctctaccg gctcagcttc gcccgttaca aaactgtaat cgatgcgtgc
     2041 ctgacctcga atcagaatac tgtctgcagg aatcagttca ttgttcctta ctatataggt
     2101 atcacctttc tgcaattccg tcacggcaac cgcttcttcc tttccatcct ccagtttagt
     2161 ggcagacaag ggaaagtacg acttgtagtc gcgggtaaaa gaaaccatct tgtaggtcct
     2221 gtcctggaag tacctgccca gcagcatgaa aaaaacgata ccagacatgg aatcgaaata
     2281 acctccgctg ccctccacga agacggtgta caggctgcgc acaaatgtga tgatgatggc
     2341 cagggcaata ggtacatcaa tatgcagaaa tccctttcgc agactcttcc agctgctgat
     2401 gtaaaattca tctgcgcagt aaaagaacac cggcaatgag aacagcagag acagatactg
     2461 gaaaagcagt ctgagagatg tttcgcccat ggcacctcct gccagatatt caggagcgct
     2521 gaacagcatg atattaccga agcagaatcc tgccacggcc atgcggatca gtcttccccg
     2581 gttgacactt ccatcgtctt tcttttccat atcctgcaaa gacagatatg gctcgtaacc
     2641 aatggtatcg agcagggaag caacctcccg cacagagatc tcttcccggg cggcgatgat
     2701 ggtgatctct ttccgacgga aatcaacacg ggcctgtgcc acggcaggat gcagggtgtg
     2761 gagattctcc agtaaccaga tacaactgct gcaatgcatg tgcggcaggt agaaacgcat
     2821 atgcatctgc cgtgcatcct cgaaggtcac aaatcgtgcc cgaatatcgg gtctgtcaag
     2881 gatctcatat ttggatgtct cgaccgcatc acgcctgttc ttaccaggag cagattccag
     2941 ttcatagtac gcacagaggt cattatctgc caggatacca tagacggtct tgcagccgaa
     3001 acagcaaaat ggcttttcgt ccacataata gacctcttcc gtgcagtctt cgccacagtg
     3061 gtaacaggtc agtctggtgg tggtctctcc ctgccttgcc atgaatgata cgaaggtaag
     3121 tacctgcgga ggcccctgca ggtgatatat gtcatacttc cggcttattt gtaccacccc
     3181 gcgagcaact tttaggtagc tttgacgttc atttcaggtc atgcgccgaa tattacccct
     3241 tgtgatgctt ttcatcgttt ctgtggcagg taaccatgtc ctggcccagc tcacagagat
     3301 cgatcccaaa gatgacaatg aacctcgaca gaccgagtct gttgaaaagg ataaagacaa
     3361 gaaggacagc aggttcgttt acggaggttt catggcagcc gggtttggca actacactac
     3421 gatcaatctg tcgccggtgg taggctaccg ggtgaccgat aaattcacac caggcatcgg
     3481 attcacctgg cagtatgcac aatacaagga tgctgataac atggtttaca acccctttgc
     3541 agactacaag ttcaatactc ttgggggcag ggtctttatg cagtattcca ttctgtacgg
     3601 tgtattcgca catgcagagt atgagcaact ctggtacaac tttgagtttc tggaatcacc
     3661 ttacctgcag ataaatgatc agcagcccgg actatttctg ggtggtggat atcgctttgg
     3721 agcaggcaat tctgccggcc tgcagatcct ggccttgtgg aatgctcttt gggatacaga
     3781 caaccttatt tatcccaacc cctggaccct gaggttcagc gtgatgttct gatcatacat
     3841 ccagtttggc gtagcgggcg ttcgcttcga tgaactcacg tcggggcgga acttcatcac
     3901 ccatcagcat actgaagatc ctgtctgcct cagccgcact ttctatggtc acccttctaa
     3961 gggtgcgggt ttccgggttc atggtggttt cccacagctg ttctgcgttc atttcaccaa
     4021 gacccttata gcgctgaatg ttaactgatt cttctttgcc accttttgcc agttcggtca
     4081 cggcttcttt ccgctgttct tcattccagc agtatttaaa gtcctttccc ttcttcacca
     4141 tatataaagg tggagtggcg atgtagatat atcccagctc aacaagctca tacatcttgc
     4201 ggaagaagaa ggtgaggatc agggtggtga tgtggcttcc atccacgtcg gcatctgtca
     4261 tgatgacgat cttgtggtag cgcagctttg tagtgtcgat ggatttgtct tcgttgggca
     4321 taacacccag ggctgtaaaa atattcttga tctcttcgtt gtcgaagact ttataatcca
     4381 atgctttttc tacgttcagg atcttaccac gaagcggaag aatggcctgt gtatggcgat
     4441 cacgaccctg tttggctgta ccacctgcag aatcgccctc caccaggaag agctcacatt
     4501 tttcagggtc tttttcggca caatcggcca gttttccggg cagtccggtg ctgcttaagg
     4561 caccttttcg ctgcaccagc tcacgggcct ttctggcagc ggttctggct tgagcagcca
     4621 gcacgacctt gccgatgatc atcctggcca tcttggggtt ctcttcgagg tagttattca
     4681 gggcctcacc ggcaatggta tctacgatac ccagtacctc actattgcca agttttgttt
     4741 tggtctgccc ttcgaactga ggttcaggca ccttgactga aaccagagcg gtaagccctt
     4801 cgcggaagtc gtcgccggta atatccactt tggccttttc aaacagacct tccctgtcgc
     4861 cataagcctt gaaggttctg gtgagtgccc gacggaagcc tgatacatgc gttccgcctt
     4921 caatagtatt gatattattg acgtaagagt ggatgttctc gttgtagctg tcattgtact
     4981 gcaaagccag ttctacctgt acaccatctc tggcaccttc ggtataaatg ggttcaggta
     5041 taatggaact cctgctgcca tccagatacc tgacaaagcc tgtcagtcca tcttctgaaa
     5101 agaactcttt actaaggaag gatccgtctt cttgtttttc acgttcatct gtcagagaga
     5161 gtttgatgcc cttgttgagg aaagccagtt cgcgaagtct ggcagccagg gtatcaaatt
     5221 tatattcggt aacggtgaag atggtatcat ccggcctgaa agtgaccatg gtaccggttc
     5281 ggtcggtctc tccggcttct tttaccgcat acagtggttt acctatgctg tactcctgaa
     5341 tatagatctt gccatttcgg tgcacttctg ctttcaggtg ggtggacaat gcgttcacac
     5401 atgacacccc cacaccgtgc aaaccgcctg acactttata ggtgtcttta tcaaatttac
     5461 caccggcatg gagtacggtc atgaccactt ccagtgctga tttcttttct ttctgatgga
     5521 tctctgtcgg gatacccctt ccatcatcct gcacagtgat ggaattgtcg gtatgaatgg
     5581 tgaccagtac tgtgctggcg tatcctgcca gtgcctcatc aatggagtta tctaccacct
     5641 catacacaag gtggtgcaaa cccttcacgc cggtatctcc gatatacatg gccggccttt
     5701 tcctgaccgc ttccagccct tccagtacct ggatattatc agctgtatag ttgttgcttt
     5761 ttggtgcgtt catccctgca gaatatctct ttttatagac ccgtcaaaga taccacattc
     5821 aggccgtaag acacaggtgt ccagggaggt tttttccacc tgttaaggca tcgcaaaacc
     5881 ctttcctgtt aagcgtttca ggcgatctca actcattttc cgctgcgctg ggtcagcaca
     5941 tagtaatcca gattaccgga tatatttttc cgggtgtcaa aacctgcagg cagatcatag
     6001 tactgatact cctgactggg gtacaggcgc accagctgat catccacctg aatgtcataa
     6061 ggcatattca ttcccttcac gatattggcc catctgtaca ccagaactgg acctgattta
     6121 ccatcttcaa aatggtagtc aaacacaggt atatgtgtag tgcggagata ctgtgtaaag
     6181 atgcgtccga gatccattcc tgctttgcgg cttatataat cttccaccat cagggaagta
     6241 acggtctggt ggtagaaatc atgattcaaa cccctcagga tatcgcgaaa gagactatca
     6301 ttatcaatga cctgcctgat ggtgtgcaac aggttagctc ctttatagta catatcgccg
     6361 ctcccttccg tattgaccag atagggaccg atgaccggtt catcgttcga aatatttttt
     6421 cgcagtccac gcacgtattt atcggctgca tccttccctt tccagtattc aatgaataag
     6481 gtctcgctgt aatcggtaaa tccttcgtgc acccacatat ctgctatgtc tttggtggtg
     6541 atgttgttgc caaaccattc atgaccagat tcgtgcacca caatataatc ccagtcatca
     6601 ccttcgccgg tacctgaaag atctccaccc agataccctt tcttgaattt atttccgtat
     6661 gctatggcac tctgatgttc catacccaga tgaggagctt ccaccatttt gaatccgtct
     6721 tcatagaatg gataggggcc aaaccagtac tcgaatgcct ccagagtctt gttgatatca
     6781 ggtaaaacgt gactgatggc ctgatccata tgcccttcca ggaaccacat atccacgtcc
     6841 agtgtgccgg cctctccctg ataggtggaa tgatgatctt catactttcc gatatagggg
     6901 atcaggttat agttgttgat cggattgacc actgcccagt tccaggtaag tgatccatct
     6961 gcattttctg tcattccacg aaagcggcca ttagataccg cagtctgtcc tttgttggtt
     7021 atgatatgca tggcagcact gtcgggttca tcgctttgat ggtctttatt cggataccac
     7081 acgctggcac caagaccctg gcaggccaca ctgatccagc tgttgccgtt gtggtctctg
     7141 gaccagatca cgccaccatc ccagggcggc aatctggcca ctctgggttt gccgtgataa
     7201 ctgatttcca gcttatggat actgccattg gccaaagagg gtgtttgcag atagaacaca
     7261 tcatcatgcc gggatatgga aacgggttct ccatccagca gcaccctgtc cacctccagt
     7321 ggttgcatca gatctatctg catgctgcta ccggtatctt ccacccggaa actgatggta
     7381 tttttacctt ccagggtctt attttcttcg ttgaaacgga tggtcagatc atagtgggtc
     7441 acattccacc aggagcgttc aggtgtgata cttccacgga tggtgtcagc gtgtgtaaag
     7501 gatcctttat tgtcaaaaag ctgtgcctgt gcgtgcatca tcacgagcag cagaacgccg
     7561 gtactccacc atttcatagt ctggtatttc cggctaaaat acgatgttgt gatggcagga
     7621 tgtggttcct tcacatatat taacgcaatc agaaaaacca gtttacatac acagaatatg
     7681 ctccattatt ggtggtttct tctgtactgt attgctgcca aattgtatgg tcaaagcggg
     7741 caccaacgct gaaccggtca ccccggtatt ccattcctgc catgccatgt gccactgaat
     7801 aggcgctttc ttcattcaga aaatcatact tccggtatcc gttgcggtgt tctgcaacca
     7861 ggcccgcgaa gggcacccac ctgcctgagg cccccagact tctggctacc acggtctgag
     7921 cctgccagaa caatccttcc tgcaaatgat ctgcgttggg aagagaatat ttcacagacg
     7981 caccgaacat agcagtccat gcagaatctg aaagtatata ctggataccg gccaacgggt
     8041 caatacttgc tgagcccgga taaaaccccg ggggaaggcc tgcctgaaca aaatcagcct
     8101 tgtattttcc ggttggcagt tctgctccgg cctgcacaaa cagggtgtgt gtggcacggc
     8161 cggaacgcag ggtcatggcc tggtatccgg caatcaggtt cagatctcct gc
//
LOCUS       DAOMAX010000108         8190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1284_length_8190_cov_4.716779, whole genome shotgun sequence.
ACCESSION   DAOMAX010000108 DAOMAX010000000
VERSION     DAOMAX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1284_length_8190_cov_4.716779"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK742_07005"
     CDS             complement(<1..285)
                     /locus_tag="PK742_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU76375.1"
                     /translation="MKKLLVPVDFSDYTWSTVTAALDLAARIDAEVRLLHVFDDPFVE
                     SDLDDTTIRNAVSRYTENLLHKMETDAKTQMADLKTKIDGYQADRKLSVSV"
     gene            415..1305
                     /locus_tag="PK742_07010"
     CDS             415..1305
                     /locus_tag="PK742_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQU76376.1"
                     /translation="MSSVYSIKDLERLSGVKAHTIRMWEQRYGLLKALRTPTNIRYYN
                     DHDLKYLMSIALLTRKGYRISVLADKTEEEIAWMVGELTAMHTEYDAQIEGLIIATID
                     YDEDRLEKILNTCILQLGFEETFRCIVFPFLEKVGLMWVSGAILAAQEHFMSQLLRQK
                     ILVAIDGHVVRHTDKSKHFLLFLPNGEWHELTLLFLQYLLKARNHRVTYLGASVPLQE
                     VLTVAETLRPDFFYTIITSVPSGYSPDEYLNTIAGKFPDATVYASGTQFINTHHSLSD
                     NVRILNGLDNVLAGVEELAA"
     gene            1391..1906
                     /locus_tag="PK742_07015"
     CDS             1391..1906
                     /locus_tag="PK742_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQU76377.1"
                     /translation="MTRNEFHDMIIELKQPMKAFALNLTREREDALDLVQETYYRAIA
                     NQDKFHEGTNLKAWLMTIMKNIFINNYRKASKRNTILDASDNLFMLNSRDAAIANQAE
                     RDFVMKDLIRAINSLDIEYRRPFLMHYQGFKYEEIAEDLSLPLGTVKSRIFFARKQMQ
                     SYLKEKGFERY"
     gene            complement(1907..2287)
                     /locus_tag="PK742_07020"
     CDS             complement(1907..2287)
                     /locus_tag="PK742_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQU76378.1"
                     /translation="MIKRIHHIALITDDLDRSLHFYSTILGFRVLSKYFRADRNSWKV
                     DLKLGDIQLELFTFPDAPARPSWPEARGLRHLAFEVEDLQKCHAELLQNNWDAEVIRR
                     DNLTGKNFFFASDPDGTPIEFYES"
     gene            complement(2284..2658)
                     /locus_tag="PK742_07025"
     CDS             complement(2284..2658)
                     /locus_tag="PK742_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQU76379.1"
                     /translation="MSRHLELGKQGEELARNWLEKKGWKILEMNWRFGKAEIDIIAQD
                     PYGLVMIEVKTRSSGIQLPEGAVNRKKQSLMANAAAQYIYDTNWQGECRFDVLAIFML
                     PGRQPEIEHFEDAFYPNPTDDL"
     gene            2740..3465
                     /gene="lipB"
                     /locus_tag="PK742_07030"
     CDS             2740..3465
                     /gene="lipB"
                     /locus_tag="PK742_07030"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQU76380.1"
                     /translation="MELTGTRTVSFRDLGHMAYGEAWDLQERVFGDTVQRKISNRTLE
                     PSARQDTVDQLFLVEHPPVITLGKNADSRNLLITAGEKQGVEVFHINRGGDVTFHGPG
                     QLVGYPILDLDHYYTDIGRYLRDLEEVIIRTIGEYGLQGDRLPGATGVWLEPDNPFRA
                     RKICAMGIRCSRWVTMHGFALNVNTDLRFFELIVPCGITDKGVTSIEKELRQTIPMEE
                     VKGHFLRHFSDVFQCKISDGRTG"
     gene            3449..4489
                     /locus_tag="PK742_07035"
     CDS             3449..4489
                     /locus_tag="PK742_07035"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQU76381.1"
                     /translation="MAEQDEKQQQEPLEEDLTEHFRVVIDRGQEPLRIDKFLVNRLEN
                     TSRTKVQSAADAGCIRVNGQIVKSNYKVRPGDDVQLILPVREDNYSLEPEDIPLDIVF
                     EDEHLLVVNKPAGMVVHPGVGNFSGTLINALMFHLGKDNLPGDDDFRPGLVHRIDKNT
                     SGLLVVAKSETAMQHLARQFFEHTVYRRYQALVWGELEEETGTITGNIARHARFRKLF
                     DVYDDPAIGKHAVTHYRLLENLGYVALVECRLETGRTHQIRVHMQHIGHPIFNDDTYG
                     GDRIVQGTIFTRYRQFIDNCFQIIPRHALHAISLGFVHPVSGEDLLFHAPLPDDFAGV
                     LEKWRTYAAQLK"
     gene            4522..5787
                     /locus_tag="PK742_07040"
     CDS             4522..5787
                     /locus_tag="PK742_07040"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQU76382.1"
                     /translation="MMRFSSACLLACLSLLSVGSYSQVERCFSAGYLEQLSQDDPSLA
                     DLQETIRERATTQSYGRLTEDEVYYIPVVVHVVYSNTSENISDDQIYSQIEVLNNDFR
                     LLNDDADKIPADFADNAGDALIEFRLAALDPDGNPTTGITRTETSITQWDINAPITAD
                     NNSEKVKADSTGGHDPWDATCYLNIWVCDLGALILGYAAPPGTSLTKDGIVVGYKWFG
                     SGGSAVPPYNRGRTATHEIGHWLGLKHVWGDDGNSCAGSDGVADTPNQAGPTYGCLSY
                     PATDACSPNPPGIMFMNYMDYSDDTCMYMFTQGQANIMRSVLEDERASVVACALVVTN
                     DSLPPGDEPVFIYPNPTTGFAWLPIDRLDSTTIEVGVYNVLGQQVIQLTHDSRLPVGL
                     DLNDLPNGAYFVMASDGLKTVNGKVVLLR"
     gene            5830..7008
                     /gene="mqnE"
                     /locus_tag="PK742_07045"
     CDS             5830..7008
                     /gene="mqnE"
                     /locus_tag="PK742_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HQU76383.1"
                     /translation="MEKRWMQLAGGDMNLQKIVNKVLQHERISPEDGVMLYEKAGTGL
                     LGALANHIREERHGDTTYFNRNFHIEPTNLCVFSCKFCSYSRLIKQQDEGWVMTKEQM
                     LDIVRSYADKPVTEVHIVGGVLPQMNLAFFADLLREIRQLAPQLHIKGYTAVELEYMI
                     RKAKMSYREGLAFLQEAGLDSLPGGGAEIFDEEIRAEICGDKCDSNTWLEIHRTAHEL
                     GMHSNATILYGHIETYAHRIDHMERLRSLQDETGGFNTFIPLKFRNQNNQMSHLPEVS
                     AVEDLRNYAVSRIYLDNFPHIKAYWPMLGRQLAQLSLDFGVNDVDGTIDDSTKIYSMA
                     GAEEQSPVMSTRDLVEMIRNVRRKPVERDTLYHTLTDYTDIEFAEGPDGAMMALPVVD
                     "
     gene            7010..7615
                     /locus_tag="PK742_07050"
     CDS             7010..7615
                     /locus_tag="PK742_07050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQU76384.1"
                     /translation="MKELYLIRHGETDLNKKGIVQGRGVNSSLNETGRRQAAQFHEQY
                     KEAGFEKIYISSLLRTYESIEPFVQMDIPVERFAELDEISWGRHEGQVSGNSYREFHD
                     LIKAWKQGQVSNRISGGESPLEVQERLQIFLKHLDQTEEKKVLICSHGRSMRILLCTL
                     FNRTLQEMDAYPHHNLCLYVVRWDGHDARMIRENDLSHLHA"
     gene            7608..>8190
                     /locus_tag="PK742_07055"
     CDS             7608..>8190
                     /locus_tag="PK742_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76385.1"
                     /translation="MHKTSISLVSYWNTRPFLFGLQKSGLTEFFEVFLDTPSAGGKRL
                     MNNEVDIALAPVITLNSMADARLLTHYCIGAAGSVRTVCLFSHEPPETLRKVYLDAHS
                     LTSVRLIRIIYSLNNWSEPEWISADVHPGMLQSGEGALVIGDKSFPWHGQYPYVLDLA
                     EEWIRLTDTDFVFAAWITRKERDEELEIKLNDAF"
BASE COUNT         2167 a         1806 c         2091 g         2126 t
ORIGIN      
        1 aacagacact gacaatttcc ggtctgcctg atacccatct atctttgttt ttaaatcggc
       61 catttgggtt ttggcatctg tttccatttt atggagcagg ttttccgtgt atctcgaaac
      121 agcgtttcgt atagtggtgt cgtccagatc actttccaca aaaggatcat caaaaacatg
      181 caggagtctt acttctgcat ctattcgtgc tgccagatca agtgcggctg ttacagttga
      241 ccaggtatag tcagaaaaat caacaggaac caatagcttt ttcatatgct ggctttggat
      301 ctaaaataaa cactggtcag gtgcggcact atgacattca tcactctttc gatttgtgct
      361 gtttgtaaag ccttagcaaa caaaagttaa acaaactggt tatttttgta ctgtatgtcc
      421 agtgtatatt ccatcaaaga cctggaaaga ctttcaggag tcaaggcgca taccatacgc
      481 atgtgggagc agcggtacgg ccttttgaag gctttacgta cccctaccaa tatcaggtat
      541 tacaatgacc atgacctcaa atacctgatg agtatcgccc tgttgaccag gaaaggttac
      601 cggatatcag ttctggctga taaaaccgaa gaagaaattg cctggatggt aggcgagctt
      661 acagccatgc atacggaata tgatgcgcag atagaaggac tcatcattgc taccattgac
      721 tacgacgaag accggctgga aaagatcctg aatacctgta ttctgcagct cgggtttgaa
      781 gaaacttttc gttgtatcgt atttcctttc cttgaaaaag taggcctgat gtgggtttca
      841 ggagctattc tggctgctca ggaacacttc atgagccagt tgctcagaca aaagatcctg
      901 gtggccattg atgggcatgt ggtgcgccat acagacaaaa gcaaacactt tttgctgttt
      961 cttcccaatg gagaatggca tgaactgacg ctgcttttcc tgcagtatct gctaaaagca
     1021 cgaaatcacc gggtaaccta tctgggggca tccgttcctc ttcaggaagt acttaccgtt
     1081 gctgagacac ttagacctga ttttttctac accatcatta cttcggttcc aagtggctat
     1141 tctccggatg aatacctcaa taccattgcc ggcaaatttc ctgatgccac tgtctatgct
     1201 tctggtaccc agttcatcaa cacccatcac tctctgagtg ataatgtgcg tattctcaat
     1261 ggattagata atgtgctggc aggtgtggaa gagctggctg cctaatttgt ttaataaaaa
     1321 tagttaaaag ttaaacaaaa agttgcgaat cctgccgcag gtaagtatat ttgggcaaca
     1381 attcactgac atgacccgta atgagtttca cgatatgatc attgagctga aacagccaat
     1441 gaaagctttt gccctgaacc tgacaagaga aagggaagat gcactggatc ttgttcagga
     1501 aacctactac agggccatcg ccaatcagga caaatttcat gaaggcacga acctgaaagc
     1561 gtggttgatg accatcatga agaacatctt catcaacaat taccggaaag ccagtaagcg
     1621 caacaccata ctggatgcat ctgataacct gttcatgctg aacagcagag atgctgccat
     1681 cgccaatcag gctgagcggg attttgtgat gaaagacctg atccgggcca tcaacagtct
     1741 ggatattgaa taccgtcggc cgttcctgat gcactaccag ggtttcaaat atgaagagat
     1801 cgcggaagat ctgagtttgc cgctgggcac tgtgaaaagt cgcatcttct tcgcccgcaa
     1861 gcaaatgcag agctatctga aagagaaggg attcgagcgc tactgatcag ctttcataaa
     1921 attctatcgg ggttccgtcg ggatcggaag caaagaaaaa attcttcccc gtcaggttgt
     1981 ccctcctgat aacttctgca tcccagttat tctgcaggag ttctgcatga catttctgca
     2041 ggtcttccac ctcaaatgcc agatgcctga gtccccttgc ttcaggccat gatggccttg
     2101 ccggtgcatc cggaaaggtg aaaagttcca gttgtatatc gcccagtttc aggtccacct
     2161 tccaactgtt tcgatcggcc ctgaaatatt tactgagtac ccggaagcct aagatggtag
     2221 aataaaaatg cagggacctg tcaaggtcat cagtgatcag ggctatgtga tgaattcttt
     2281 tgatcacaga tcatctgttg ggttggggta aaaagcatct tcaaagtgtt cgatctccgg
     2341 ttgcctgccc ggtaacatga atatggccag tacgtcaaac ctgcattcgc cctgccagtt
     2401 cgtatcatat atatattggg cagccgcatt ggccataaga gactgttttt tgcgattcac
     2461 agccccttca ggtaactgga ttccgctgct tcgtgtttta acttcgatca tgaccaaccc
     2521 atacgggtct tgggcaatga tatctatttc tgctttgccg aagcgccagt tcatttccag
     2581 gatcttccaa ccttttttct ccagccagtt tcgggccagt tcttctccct gtttccccag
     2641 ttccagatgt ctgctcatgg aattctttgt tattttgcca gtaagcggta tgaaggtaca
     2701 caagcaggaa ctgtttgttg cctcaaacgt tgacagaaca tggaattgac aggtaccaga
     2761 acggtaagtt ttcgcgatct gggtcacatg gcatatggag aagcctggga tctgcaggag
     2821 cgggtattcg gcgatactgt gcagcgaaag atcagcaaca gaaccctgga accatctgca
     2881 cggcaggata cggttgatca gttgtttctg gtggagcatc caccggtcat tacgctggga
     2941 aagaatgctg acagtcgtaa cctgctgata accgcaggtg aaaagcaggg ggttgaggtc
     3001 tttcacatca atcgcggagg cgatgtgacc tttcatggac cagggcagtt ggtgggttac
     3061 ccgatcctgg atctggacca ttactacaca gatattgggc gctatctccg ggatctggaa
     3121 gaggtcatca tcagaactat tggagaatac gggctacagg gtgatcgctt gcctggagct
     3181 accggtgtgt ggctggaacc ggataaccct ttccgggcaa ggaagatatg tgccatgggt
     3241 attcgctgca gcaggtgggt gaccatgcat ggtttcgccc tgaatgtcaa tactgacctt
     3301 cgtttcttcg agctcattgt tccatgtggt atcacagata aaggagttac ttccatcgaa
     3361 aaggagctca ggcaaacaat tccaatggaa gaggtcaagg gccattttct caggcatttt
     3421 tctgacgtat ttcaatgtaa gatcagtgat ggcagaacag gatgagaaac agcagcagga
     3481 gccgttggag gaagatctca cagagcactt ccgggtggtg attgacagag gtcaggagcc
     3541 tttgcggata gataaatttc tggtcaacag actggagaat accagccgca ccaaagtaca
     3601 gtcagctgct gatgccggtt gcattcgtgt gaacggtcag atagtcaaat caaactacaa
     3661 ggtcaggccg ggcgatgatg tgcaactgat cctgcctgtt cgggaagaca attacagtct
     3721 tgaaccggaa gatattccgc tggatattgt ttttgaagat gaacatttgc tggtggtcaa
     3781 taaacctgca ggcatggtgg tacacccggg cgtgggaaat ttcagtggca ccctgatcaa
     3841 tgccctgatg tttcatctgg gtaaggacaa cctgccgggt gatgatgatt ttcgtccggg
     3901 gctggtacat cgcatagata agaatacttc cggtttgctg gtagtggcca agtctgaaac
     3961 tgccatgcag catctggcca ggcagttctt tgagcatacg gtgtacaggc gctatcaggc
     4021 tttggtatgg ggagagttgg aggaggaaac aggtaccatt accggaaata ttgccaggca
     4081 tgcccggttc agaaaacttt ttgatgtgta tgatgatcct gccattggta aacatgcggt
     4141 tacccattat cgtttgctgg agaacctggg ctatgttgca ctggttgaat gtcgcctgga
     4201 aacgggcaga acgcatcaga tcagagttca catgcagcat atcggtcatc ctatattcaa
     4261 tgatgacacc tacggcggtg accgcattgt gcagggaacc atttttacca ggtacaggca
     4321 atttattgat aactgctttc agatcattcc cagacatgca ttacatgcca ttagtctggg
     4381 gtttgtgcac ccggtttcgg gcgaagacct cctttttcat gcaccattac ctgatgattt
     4441 tgccggggtg cttgagaaat ggcgaaccta tgcggcccag ctgaaataaa tggtcgttct
     4501 gaaagttctt aattttgacc tatgatgcga ttttcatcag cctgtctgtt agcctgtttg
     4561 tctctgcttt ctgttggcag ttacagtcag gtggaacgct gtttttctgc cggatatctg
     4621 gagcaactca gtcaggacga tccatcgctt gcagatctgc aggaaacgat cagagagcgg
     4681 gcaaccactc aaagttacgg gcgcctgacg gaagatgaag tgtactatat tcctgttgtg
     4741 gtgcatgtgg tttacagtaa tacgtctgaa aatatcagtg atgatcagat atactcacag
     4801 atcgaagtgc tgaataatga ttttcgtctg ttgaatgatg atgcagataa gatacctgct
     4861 gattttgcag acaatgcagg cgatgctttg atcgagttca gactggcggc cctggatccg
     4921 gatggaaatc ctaccacagg tattacacga actgagacca gtattaccca gtgggatatc
     4981 aatgcaccca tcactgccga taataattca gaaaaagtaa aggcagattc aacaggaggc
     5041 cacgatccgt gggatgctac ctgttacctg aatatctggg tttgtgacct tggggcgctt
     5101 attcttggct atgccgctcc gccgggaaca agtcttacca aagatggcat agtagtaggc
     5161 tataaatggt ttggctcagg agggtcagct gttccgccat acaaccgggg gcgtacagcc
     5221 acccatgaga tcgggcactg gcttggactt aagcacgtct ggggcgatga cggaaacagc
     5281 tgtgcaggaa gtgatggcgt ggcagatacg cccaatcagg caggacccac ctacggatgc
     5341 ctgagttatc ctgcaaccga tgcctgcagc ccgaatccgc cgggtatcat gttcatgaac
     5401 tacatggatt attctgatga tacctgcatg tacatgttca cccaggggca ggccaatatc
     5461 atgcggtctg ttctggaaga tgaaagggcg tctgtggttg cctgtgcttt ggtggtaacc
     5521 aatgattctt tgccacccgg cgatgagcca gtctttattt atccgaatcc aacaaccggt
     5581 tttgcctggc tgccgataga cagacttgac agcaccacaa tagaagtagg tgtatataac
     5641 gtgttagggc aacaggtcat tcagcttacc catgacagca ggttgcctgt tggtctggac
     5701 ctgaacgatc tgcccaacgg agcctatttt gtcatggcat cagatggtct caagaccgtg
     5761 aatggaaaag ttgtgctgtt gcgttgaacg cagccgtaca gatcttgttt agaagttaag
     5821 atgagtgcaa tggaaaagcg ctggatgcaa ctggcaggtg gtgatatgaa cctgcaaaag
     5881 atcgtcaata aggtgttaca acatgaacgt atcagcccgg aagacggagt catgctgtat
     5941 gaaaaggcag gcaccggctt gctgggtgcc ctggctaatc atatccggga ggaaagacat
     6001 ggtgatacca cctacttcaa ccggaatttt catattgaac caaccaatct ctgcgtgttc
     6061 agctgcaagt tctgctctta ttccaggctg atcaagcaac aggatgaagg ctgggtcatg
     6121 accaaagagc agatgctgga cattgtccgg tcttatgctg acaagcctgt aacggaggtg
     6181 catattgtgg ggggagtatt gccacagatg aacctggcat tctttgcaga ccttttgaga
     6241 gagatcaggc aactggcacc gcaactgcat atcaaaggtt atactgcggt agagctggaa
     6301 tatatgatcc gcaaggctaa aatgagttat cgtgaaggac ttgctttctt gcaggaagct
     6361 ggtctggatt ctttgcctgg cgggggtgca gagatctttg atgaagagat tagggcagag
     6421 atctgtggag ataaatgcga cagcaatacc tggctggaga tccaccgcac tgctcatgag
     6481 ttgggcatgc acagcaatgc caccattctg tatggtcaca tagaaaccta tgcacatcgt
     6541 attgaccaca tggaaaggct gcggtctctg caggatgaaa ctggtgggtt taataccttc
     6601 attccgctta agttccgcaa ccagaataac cagatgagtc atttgccgga agtatcggct
     6661 gtggaagacc tgcgcaatta tgccgtatct cgcatctacc tcgataattt tccgcatatc
     6721 aaagcctact ggcctatgct gggaaggcaa cttgcgcagc tttcacttga tttcggagtg
     6781 aatgatgtgg atggtaccat tgatgacagc acgaagatct acagcatggc cggagccgaa
     6841 gaacaatcac ctgtcatgag cacaagagac ctggttgaaa tgatcagaaa cgtgagacgc
     6901 aaaccggttg aaagagatac gttgtatcat accctgactg attatacaga cattgaattt
     6961 gcagaagggc ctgatggtgc catgatggca ctgcctgtag tggactgaca tgaaagaact
     7021 gtatctgatc agacacggcg aaacagacct caacaaaaag ggtattgtgc agggcagagg
     7081 tgtgaacagc tcgctgaatg aaaccggaag gaggcaggca gcgcaatttc atgaacagta
     7141 taaggaggca ggttttgaaa agatctacat ctcttcactg ctgcgtacct atgaaagcat
     7201 cgagcctttt gtacaaatgg atattcctgt agaaaggttc gcagaacttg atgagatcag
     7261 ctgggggcgt catgaaggac aggttagtgg aaattcttac cgtgagtttc acgatctgat
     7321 caaggcctgg aaacaagggc aggtcagcaa caggatcagc ggaggggaga gtccgcttga
     7381 agttcaggaa agacttcaga tctttctgaa acatctcgat cagaccgaag aaaagaaggt
     7441 gcttatctgt tcgcatggcc gaagtatgcg catcttgcta tgtactttat tcaacagaac
     7501 attacaggaa atggatgcct atccgcatca taacctgtgt ttgtatgtgg tcagatggga
     7561 tggccatgat gcccggatga tcagagaaaa cgacctgagc cacctgcatg cataagactt
     7621 ccatatcact cgtctcttac tggaatacca ggccttttct gtttggttta cagaagtcgg
     7681 gtctgacaga attctttgaa gtgtttctgg atacaccatc ggccggaggt aaacggctaa
     7741 tgaacaacga agtagatatt gcgctggccc cggtcatcac cctgaacagc atggcagatg
     7801 ccaggttgct gacccattat tgtataggag cagccggcag tgtgcgcact gtttgcttgt
     7861 tttcgcacga gcctcccgaa acattaagga aagtatatct cgatgcacat tcactgacat
     7921 cggtccggtt gatcaggatc atttattcat tgaataactg gtcagaaccg gaatggatat
     7981 cggcagatgt acaccccgga atgctgcaat cgggtgaagg agcactggtc attggcgata
     8041 aaagttttcc ctggcacgga cagtaccctt atgtactgga tcttgcagaa gagtggattc
     8101 ggctaacaga tactgatttt gtttttgctg cctggatcac gaggaaggaa cgagatgaag
     8161 aactggaaat aaaactgaat gacgcttttg
//
LOCUS       DAOMAX010000109         8171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1289_length_8171_cov_3.684081, whole genome shotgun sequence.
ACCESSION   DAOMAX010000109 DAOMAX010000000
VERSION     DAOMAX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1289_length_8171_cov_3.684081"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PK742_07060"
     CDS             complement(<1..697)
                     /locus_tag="PK742_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76386.1"
                     /translation="MSPMHPMYKKLAAMSGMAVGFLSVQTAAGQVLFHDIIPDTEVEL
                     DGDDPVIPVDVDMDGLTDLYYYGSYWSWCYVCGESWNFWIEPTEETIIGGALVPADTC
                     VMFSWTEYEDSFYGVANLMEGFPIPGSLNWSENPSDLFYAASSSYGAFCSNGMTGDGY
                     VPVQLIKPDGMHYGWVRLIASLGGGIEGHGYIQLLETAYQTGTDESLNTGLEYLSDAF
                     EPARSLDHELVSLD"
     gene            775..2928
                     /locus_tag="PK742_07065"
     CDS             775..2928
                     /locus_tag="PK742_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQU76387.1"
                     /translation="MKKTMLLLLLATTARLFAQTGAITLEDLWLNYRYYPSYIADIRS
                     MNDGEHYTVAEGDAIVRYAYATGEMVDTVFSVTMTNGALTSFDGYAFNDDESALMLST
                     ETDSRYRYATFENNYIYDRNAKTTKALTGTGKQMYADFAPAGNRVAYVIDNDLYYTDL
                     NSSKETRVTSDGELNHIINGGSDWVYEEEFALVRSFEWSPDGKKIAYYRFDESEVPQF
                     NMTMYRGQLYPYDYTFKYPKVGEVNSQVSIWIYDLASGRKVQAQTGNWEYIPRIKWID
                     NNQLCVTTMNRLQNDLVLQNVDAATGASKKLFEEKADKYIEINDDLTFLPDGKGFIWR
                     SEKDGYSHLYHMDMNGQVVKQITSGNWDVSEFKGFDAATKALYYVAGETTPMQRQLYV
                     TQLDGKKKMLLGPADGWNQADFSDGFKYFINVHTTLNTPPVFDLYTGKGKMIRTLEDN
                     KELSDKMAQLNMKPAEFFQMTTTDNMTLNGYMIKPADFDPNKKYPVFMYVYGGPGSQT
                     VMDTYGGFNYFWFQMLAQNGYIVVSVDNRGTGARGRDFRTVTYENLGKYETMDQIEAA
                     RWLGKQSYVDASRIGIFGWSYGGYMTALCLTKGADVFKAGVSVAPVSNWKYYDNIYTE
                     RYMGTLETNPDGFDANSPINFTDMMKDNSMLLVHGTGDDNVHFQNSAELINALVRSNK
                     QFDLMVYPDRNHGIYGGKTRYHLYKKMTDYILENL"
     gene            complement(2976..4694)
                     /locus_tag="PK742_07070"
     CDS             complement(2976..4694)
                     /locus_tag="PK742_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQU76388.1"
                     /translation="MKFFAGTLLAMVWLIPSQAQITQYSQDNRTPEYAEVISYYKSVA
                     ASSSLADIQEVGMTDAGYPLHTVILDADGDFDPGISRTKNKTILLINNGIHPGEPDGI
                     DACIQLVDNWVSGKQPFPDNVVVVIIPVYNIGGALNRSSHSRANQNGPQEYGFRGNAR
                     NYDLNRDFIKQDTRNARSFAAIFQHWHPDIFIDTHVSNGADYQHVMTLVPTQHDKLMT
                     PLGTFLTDEVSPYLYDAMEEAGFPMCPYVNTIGESPESGIVGFMDHPRYSTGYAALFN
                     TIGFMTETHMLKPYDQRVQATKAFITSCVAYMTTHSNAIIQMKDSADIMAQKQYIRPI
                     EYVLDTSKVDSFLFKGYTAGHKLSQVSGAQRLYYDRDQPWEANIPLYDHYKPKMSMYY
                     PEAWILPFAWEEVTERLLISGVKMHQLQQDSTMTVEFWYIDSLGTGTNSYEGHFRHQH
                     VTGHLQREEMMIPAGSWYIDAHQPARRFLMEVLEPVSPDSYFSWNFFDPILMQKEWYS
                     PYVFEDIAAELLRTVPGLSIQLSLAMQDDPSLKDPQMQLYWVYKHSDYFEKTYMRYPV
                     MRVVEK"
     gene            complement(4691..5101)
                     /locus_tag="PK742_07075"
     CDS             complement(4691..5101)
                     /locus_tag="PK742_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76389.1"
                     /translation="MKKLLFAPALFLLMSFSSPQPPAKPATIIISYEFKGIEEGYDHQ
                     TKSVVKVDGKDVLTTEVHKQSQPMKCSVEVAPGEHDIRIDTWSMWEGNWELHTVENGY
                     SIDCNYAFHDTYKKKKTYKYHVVYDLDKGTYLGE"
     gene            complement(5109..5744)
                     /locus_tag="PK742_07080"
     CDS             complement(5109..5744)
                     /locus_tag="PK742_07080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQU76390.1"
                     /translation="MAHPIITVEGNIGSGKTSLSERLAKDLNATLILETFADNPFLQK
                     FFVRQQDYALGMEMFFMAERYEQIKDAVPDWDQLQQPVVIDYLFTKSLLYAQANLDED
                     EFHVFGKIFDILNPKLPTPDLIIYLHSNLDRLVANIHQRGREFELTVKKDYLQKIEGQ
                     YMRYFEQQPDCPVLLLDVSQADFVKQEIHYRQIHALMDRQHQPGIHRYTIK"
     gene            complement(5774..6277)
                     /gene="folK"
                     /locus_tag="PK742_07085"
     CDS             complement(5774..6277)
                     /gene="folK"
                     /locus_tag="PK742_07085"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQU76391.1"
                     /translation="MQHTGWLLLGTNLGKRLENLQAATGAIRTFLGSVEVQSHIYETA
                     AWGKTHQPAYYNQAIRISTPCTALETLHHIKQIEFLLGRKHDEKWSPRVIDIDILFFD
                     DISITSPLLTIPHALLHERRFALEPLHEIAPELVHPTLDLSVTQMLALCKDNSEVKLL
                     EELHWEF"
     gene            6360..8126
                     /gene="sppA"
                     /locus_tag="PK742_07090"
     CDS             6360..8126
                     /gene="sppA"
                     /locus_tag="PK742_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQU76392.1"
                     /translation="MNTFWKSFFATLLALVVFSLLMFFIVLGMVAAVTSGLEKKAVAV
                     ESHSILYLDLNYPIPEQTSFHIPFPSASIFSADEGAGLNDILRSIEAAADDPNIEAIW
                     LQMGYNVNPYATLQEIRNALQAFRINSDKPVIAYGEVVNQSSYYLGSVADEVYLNPAG
                     VLEFKGLAARLTFIKGTLDKLGIRTQVFWAGEFKSATEPLRLDKMSDQNREQLREYVG
                     GLYDQILADISVSRKMPEDSLRSVANELMAWLPVQTVRTGLVDSLLYDDQLRSMLQER
                     LELEEDAELVSVADYVESIPKQSGNWENQIAVVYMEGDIVDGSGQNGSIGSVSYRKIL
                     SDLRQDDEIKAVVLRINSGGGSAVASEVIWREIEALRAVKPVVVSMGDYAASGGYMIA
                     CSADKVFAQPNTLTGSIGVFMILPDISGFREDKLGMTSDTLLTGKFSDFPTISRPVSD
                     AEAVKLQEGIDSAYINFKRLVANGRNMDMNAVEDLAKGRIWLGTTALNLGLVDALGSK
                     EDAIQAAADLAELDTYRIHEYPEQKQELWEELFNSITNEQAEAAVWQEKLGDLFEPYL
                     QVQDYLQHPVMRTELPYEVEVY"
BASE COUNT         2092 a         1981 c         2123 g         1975 t
ORIGIN      
        1 catccagaga aaccagctca tgatccaggg aacgagctgg ttcaaatgca tcggacaaat
       61 actcaagacc agtattcaga gattcatctg taccggtttg ataagctgtt tccagcagtt
      121 ggatgtaacc atggccttca atgccacctc caagagaagc gatcaggcgc acccaaccat
      181 aatgcatacc atctggtttt ataagctgaa ccggtacgta accatcacca gtcatgccgt
      241 tggagcaaaa tgctccgtaa gaagaggaag cagcatagaa aagatcagac ggattctcac
      301 tccagttcag tgagccggga attggaaagc cttccatcag attggccaca ccatagaagg
      361 aatcttcata ctctgtccac gaaaacatga cgcaggtatc tgctggaact aatgcccctc
      421 caattatggt ctcttcagta ggttcgatcc aaaagttcca ggactctcca caaacataac
      481 accaggacca gtaagaccca taataataca gatcggtcaa accatccata tccacatcaa
      541 ccgggatcac aggatcgtct ccatccagtt ctacttcagt atccgggatt atgtcatgaa
      601 ataagacctg tcctgcagct gtttggacag aaagaaatcc cacagccatt ccactcatgg
      661 cagccagctt cttatacatg gggtgcatgg gagacacagg cctgattttt ggagttatgc
      721 attcaaatct atgaatttaa caggatttag acgccaaatg ctgaacttta cctcatgaag
      781 aagaccatgc tcctcctgct cctggccact actgccaggc tctttgccca gaccggtgcc
      841 atcaccctgg aagacctgtg gctgaactac cgatattatc cctcatacat cgctgatatc
      901 cgcagcatga atgatggtga acactatacc gtggccgaag gcgatgccat cgtacgttat
      961 gcctatgcca caggagagat ggtggatacc gtattctccg tgaccatgac caacggtgcg
     1021 ctgaccagct ttgacggcta tgcattcaat gacgatgaat cggccctgat gctgtcaacc
     1081 gaaacggatt cccgttatcg ctatgccacc tttgagaaca actacatata cgaccgtaat
     1141 gcgaagacca ccaaagccct gacgggcacc ggcaagcaga tgtatgccga tttcgctccc
     1201 gccggcaatc gggtggctta tgtcattgac aatgatctgt attataccga tctcaacagc
     1261 agcaaggaga cccgggtgac cagcgatggc gaactgaatc acatcatcaa cggtggttcc
     1321 gactgggtgt atgaagagga atttgccctg gtgcgttcct ttgagtggag tcctgatggc
     1381 aagaagatcg cctactatcg ttttgacgag tcggaggtgc cgcagttcaa catgaccatg
     1441 tacagaggtc agctgtatcc ttacgattat actttcaaat atccgaaagt aggcgaggtg
     1501 aacagccagg tgagcatctg gatctatgac ctggctagtg gcaggaaagt gcaggcgcaa
     1561 accggtaact gggaatacat cccacgcatc aaatggatcg ataacaacca gctctgcgtg
     1621 accactatga acaggctgca gaatgatctg gtgctgcaga atgtagatgc tgctaccggt
     1681 gcatccaaaa agctgtttga agaaaaggcc gacaagtata tcgagatcaa cgacgatctg
     1741 accttcctgc cggatggcaa gggcttcatc tggagaagtg aaaaggatgg atacagccac
     1801 ctgtatcata tggacatgaa cggacaggtg gtgaagcaga tcaccagtgg caactgggat
     1861 gtatctgagt tcaaaggctt cgatgctgcc accaaagctt tgtattatgt agccggagaa
     1921 accacaccga tgcagcgtca gctgtatgtg acccagctgg atggcaaaaa gaaaatgctg
     1981 ctcggcccgg ccgatggctg gaaccaggct gatttcagtg atggattcaa gtatttcatc
     2041 aatgtgcaca ccacactgaa taccccgcca gttttcgacc tctacaccgg aaaaggcaaa
     2101 atgatccgca ccctggaaga caataaggaa ctcagcgata aaatggctca gctgaatatg
     2161 aagcccgccg agttcttcca gatgaccacc acagataaca tgaccctgaa tggctacatg
     2221 atcaagccgg cagatttcga tccgaacaag aagtatccgg tgttcatgta tgtgtatggt
     2281 ggaccgggtt cgcagaccgt tatggacacc tatggtggtt tcaactactt ctggttccag
     2341 atgctggcgc agaacggtta catcgtcgtg agtgtagaca acagaggaac cggcgcacgt
     2401 ggccgcgact tccggaccgt cacctatgag aacctgggta agtatgagac catggaccag
     2461 atcgaagcgg ccagatggct gggcaaacag tcttatgtgg atgcatcccg catcggcatc
     2521 ttcggctgga gttatggtgg ttacatgacc gccctctgcc tgaccaaagg agcagatgta
     2581 ttcaaggcag gtgtgtctgt agctcctgtc agcaactgga agtactacga caacatctac
     2641 accgaaagat atatgggtac gctggagacc aaccccgatg gattcgatgc gaattcaccc
     2701 atcaacttca ccgacatgat gaaggataac tccatgctgc tggtgcacgg taccggcgat
     2761 gacaacgtcc acttccagaa cagtgcagaa ctcatcaacg ccctggtgcg cagcaacaaa
     2821 cagttcgacc tgatggtcta tcccgaccgc aaccacggca tctatggtgg caagaccagg
     2881 tatcatctgt ataagaagat gacggattat attctggaaa acttgtgatc aagtaaacgg
     2941 caaaaagcaa agggtggaat agttccaccc tttttttatt tctcaactac ccgcatcacc
     3001 ggataccgca tgtacgtttt ctcaaaatag tcggagtgtt tgtacaccca gtacagctgc
     3061 atctgcggat ctttcagtga tgggtcatcc tgcatagcca ggctcaattg tattgaaagt
     3121 cccggaacag ttcgcagcaa ttccgctgcg atgtcttcaa acacataagg actgtaccat
     3181 tccttctgca tcagaatggg gtcgaagaaa ttccagctga aataggagtc aggagaaaca
     3241 ggctccagta cttccatcag aaaccgacgg gcgggttgat gtgcatcgat ataccaggaa
     3301 ccagccggga tcatcatctc ttctctttgc aaatggccgg tcacatgctg atgccggaaa
     3361 tgcccttcat atgagttggt gcctgtgccc agagaatcaa tgtaccagaa ttcaaccgtc
     3421 atggtgctgt cctgctgaag ctgatgcatc tttaccccgc ttatcagtag tctttctgtc
     3481 acctcctccc aggcaaacgg cagaatccag gcttcggggt aatacatgga catttttggt
     3541 ttgtagtgat cgtacaaggg tatgttggct tcccatggct gatcgcggtc gtagtaaagt
     3601 ctttgggcac cacttacctg actaagttta tgaccggcag tatatccctt aaagagaaat
     3661 gaatcgacct tgctggtatc cagtacatat tctattggtc tgatgtattg tttctgcgcc
     3721 atgatatcag ctgagtcctt catctgtatg atggcattgc tatgggtggt catatacgcc
     3781 acacaggagg taatgaatgc tttggtagcc tgtacccgct gatcataggg tttgagcata
     3841 tgggtctctg tcatgaaacc gatggtgttg aagagtgctg cataacccgt gctgtaccgc
     3901 gggtggtcca tgaaaccaac gataccactt tcgggcgatt caccaatggt gttgacatac
     3961 gggcacatcg ggaaaccggc ctcctccatg gcatcatata agtatggcga tacctcatcc
     4021 gttaaaaaag taccaagagg ggtcatgagc ttatcgtgct gggtgggcac cagggtcatg
     4081 acatgctggt agtcggcgcc attgctcaca tgggtgtcga tgaagatatc cgggtgccag
     4141 tgctgaaaga tggctgcgaa cgaccgggca ttccgggtat cctgcttgat gaagtcgcgg
     4201 ttgaggtcgt agttgcgggc atttccccgg aaaccatatt cctgcggacc attctggttg
     4261 gcacgggaat gactgcttcg gttcagggcc ccaccgatat tatacaccgg aatgatgacc
     4321 accaccacat tgtcgggaaa gggttgcttt ccgctcaccc agttatcgac cagctggata
     4381 caggcatcga taccatcggg ttcacccggg tgaataccgt tgttgatgag cagaatggtc
     4441 ttgtttttcg tcctggatat tccgggatcg aaatcgccat cggcatccag tatgaccgtg
     4501 tgcaacgggt agcctgcatc ggtcattcca acttcctgta tatctgccag tgacgaagaa
     4561 gcagcaacac ttttgtaata agaaatgacc tccgcatatt ccggagttct gttatcctgg
     4621 ctgtattgag tgatctgtgc ctgcgatggg atcagccaca ccatcgccag gagggtacct
     4681 gcaaaaaact tcattcacca agatacgtac ccttgtccag atcgtacacg acatgatact
     4741 tgtaggtctt cttcttcttg taagtgtcgt ggaatgcgta gttgcagtcg atgctgtagc
     4801 cgttttctac ggtatgcaat tcccagttac cttcccacat gctccaggtg tctatgcgaa
     4861 tgtcgtgttc acccggggct acctctactg aacatttcat gggttggctt tgcttgtgta
     4921 cctcggtggt aagtacgtct ttaccatcta ccttgaccac agatttcgtc tggtgatcat
     4981 agccttcttc gatccctttg aactcataag agatgatgat ggtagcaggt ttggcaggag
     5041 gctgcggaga ggagaaactc atcagcagaa acagggcagg ggcgaagagc agttttttca
     5101 ttggtgtgtt atttgatggt gtaacgatga ataccaggtt ggtgttgcct gtccatgagc
     5161 gcatggatct ggcgatagtg tatctcctgc ttgacgaagt cggcctggct tacgtcgagc
     5221 aacaggacag gacaatcagg ttgttgttcg aagtaacgca tgtactgtcc ttcgatcttt
     5281 tggaggtaat ccttcttaac agtgagctca aactcccttc cccgctggtg gatattggca
     5341 accagccggt ccaggttgga atgcaggtaa atgatgagat caggggtagg tagtttcgga
     5401 ttcaggatat cgaagatctt tccgaacaca tgaaattcat cctcatccag gttggcctgc
     5461 gcatagagca gagacttggt gaacagatag tctatcacca ccggctgctg gagttggtcc
     5521 cagtctggca cggcatcctt gatctgttca tagcgctctg ccatgaagaa catctccatg
     5581 cccagggcat agtcctgctg acgaacaaag aatttttgca ggaagggatt gtcggcaaac
     5641 gtttccagga tcaaagtggc attcaggtct ttggccagac gttcagataa agaggtcttg
     5701 cccgatccga tattgccttc taccgtgatg attggatgcg ccatgccgca aaactacatg
     5761 acgaagggat aactcagaat tcccagtgca attcttccag aagcttcacc tctgaattgt
     5821 ctttgcaaag tgccagcatc tgggtaacgc ttagatccag ggtagggtgc accagttcag
     5881 gtgcgatctc atgcagtggc tccagagcga aacggcgctc gtgaaggagt gcatgtggta
     5941 tcgtgagcag cggagaggta atggaaatat cgtcgaagaa caggatgtct atgtctatga
     6001 ccctgggcga ccacttctca tcgtgcttcc gtcccagcag gaattctatc tgcttgatat
     6061 ggtgcagcgt ttccagtgcg gtgcagggag tgctgatgcg aatggcctga ttgtaataag
     6121 cgggctgatg cgtcttgccc caggcagctg tttcgtaaat atgagattgt acttctaccg
     6181 atccgaggaa agtgcgtatc gctccggtgg cagcctgcag gttttccagg cgcttgccta
     6241 aatttgttcc gaggagtaac cagcctgtgt gttgcacggt ctatttactg agattacaat
     6301 gatacaaacc taatgaaaaa acaggttgtt tattgtaaca gaactgacaa atcactgaca
     6361 tgaatacttt ctggaagtct ttttttgcca ccctgcttgc cctggtggtc ttcagcctgc
     6421 tgatgttctt tatcgtactg ggcatggtag cagcggttac cagtggtctg gagaaaaaag
     6481 cggttgctgt agaatcccat tccatcctgt atctggacct taactatccc atcccggaac
     6541 agacctcttt ccacattccc ttcccctctg caagcatttt cagtgctgac gagggcgccg
     6601 gactcaatga tatcctgcgg tccattgagg cggctgctga tgaccccaac attgaagcca
     6661 tctggttgca aatgggctac aatgtgaacc cctatgccac ccttcaggag atcagaaatg
     6721 ccctgcaggc tttccgcata aatagtgaca agccggtcat cgcctacgga gaggtggtga
     6781 accagtcgtc ctattacctg ggctctgtgg ctgatgaggt ctatctcaac ccggccggtg
     6841 tgctggaatt caaaggcctg gctgccagac tcacctttat caaaggaacc ctggacaaac
     6901 tcggaatccg cacccaggta ttctgggcgg gtgaattcaa aagtgctacg gagccgctcc
     6961 gcctggataa gatgagcgac cagaaccggg aacagctgcg ggagtatgtg ggcggactct
     7021 atgaccagat actggccgat atcagcgtgt ccagaaagat gccagaagat tcccttcgct
     7081 ccgttgccaa tgaactgatg gcctggttac ccgtgcagac cgtgcgtacc ggactggtgg
     7141 acagcctgct gtacgatgat cagctgcgca gtatgctgca ggaaagatta gaactggaag
     7201 aagatgctga actggtttcg gtggccgatt atgtggagag catccccaaa caaagcggca
     7261 actgggagaa ccagatagca gtggtctata tggaaggcga tattgtggat ggtagcggac
     7321 agaacggttc tatcggttca gtaagctacc ggaagatcct gagcgacctg cgtcaggacg
     7381 atgagatcaa agcagtggta ctgcgcatca attccggcgg agggtcggca gtagcaagtg
     7441 aggtgatctg gcgggagata gaagccctga gagcggtcaa acctgttgtg gtcagcatgg
     7501 gcgactatgc tgccagcgga ggatacatga tcgcctgtag tgccgataaa gtatttgccc
     7561 agcccaacac cctcacaggc agcatcggcg tattcatgat attacctgat atttccgggt
     7621 tccgcgaaga caaactcggc atgaccagcg ataccctgtt aacaggaaag ttctctgatt
     7681 ttcctaccat cagccgaccg gtctcagatg cagaagcggt caagctgcaa gagggtattg
     7741 acagtgctta tatcaatttc aaacgcctgg tagccaacgg cagaaatatg gatatgaatg
     7801 ccgtggagga tctggcaaaa ggcaggatct ggctgggaac aactgccctg aatctgggcc
     7861 ttgtggacgc tctaggaagt aaggaagatg ccattcaggc tgcggcagat ctggcagaac
     7921 tggataccta cagaatacac gagtaccccg agcagaaaca ggagttgtgg gaagagttat
     7981 tcaacagcat caccaatgaa caggcggaag ctgcagtctg gcaggaaaaa ctgggcgatc
     8041 tctttgaacc ttacctgcag gttcaggatt atcttcagca tccggtcatg cggacggaac
     8101 tgccgtatga agtggaggtg tattagttgg gtattgggta ttcggtattg ggtattgggt
     8161 attgggtagt g
//
LOCUS       DAOMAX010000110         8163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1290_length_8163_cov_4.060434, whole genome shotgun sequence.
ACCESSION   DAOMAX010000110 DAOMAX010000000
VERSION     DAOMAX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_8163_cov_4.060434"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PK742_07095"
     CDS             <1..423
                     /locus_tag="PK742_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQU76393.1"
                     /translation="DVIFKPLDGMGGSAIFRVKPGDSNLSVILEMLTLQQQLSARWQV
                     TIVLKRAYTCITTPDGKIYINGSGNPGMATAGSGDVLTGIIAAWMAQGLSPTDAAIAG
                     TYLHGLAGDLAMKEQGGLNIIAGDIIDHYNSAIKILRA"
     gene            420..1100
                     /locus_tag="PK742_07100"
     CDS             420..1100
                     /locus_tag="PK742_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HQU76394.1"
                     /translation="MKIVVLSGAGMSAESGIATFRGAGGLWEGHRVEDVATPEAWQKN
                     PELVLQFYNERREKIRHAQPNKGHTYLAEMEKEHEVFIITQNIDDLHERAGSTQVLHL
                     HGEIMKSRSEKYTEPLYDQPGHISTGDLCERGTQLRPHIVWFGEPVPMMEPAIAIVST
                     ADLLLITGTSLVVYPAASLWHYAPAGCPVYLVDPNPPAGLPAHVRTIRETAVNGIQLF
                     RKMISHGT"
     gene            1090..1632
                     /locus_tag="PK742_07105"
     CDS             1090..1632
                     /locus_tag="PK742_07105"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQU76395.1"
                     /translation="MEHNPWTTLQREEQYRNNWIQVEEHQVLNPAGNPGIYGVVRFAA
                     YAIGIIPVDTFGNTWLVGQYRYPLEAYSWEIPEGGGNKNDTPERSALRELQEETGVMA
                     GRLELLAHLHTSNSVTDEEAFIYLATDLTEGQSSPEETESLQIRKLPLTEAIQMAMDG
                     RITDAMSVAGLLKAKLVLGL"
     gene            1634..3754
                     /locus_tag="PK742_07110"
     CDS             1634..3754
                     /locus_tag="PK742_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76396.1"
                     /translation="MQQKLVKSILGVGAFLFVCWSFVFCKQQETGAVTPVAADVAGFV
                     GDQVCGSCHAEAYADWKGSHHFHAMELPDSNSVRGRFDGSTFTADGVRYRMYQQNGQY
                     LFDITENGQATEQFPVAYTFGWEPLQQYLVARPGGRYQTLRISWDTEKQQWFHQYPDT
                     VITPGDWLHWTGAAQTWNSMCAACHSTHLEKNYIAEKDSFHTTWSIINVSCEACHGPG
                     EQHVALATKGDWRNGAGISTFSKDGTHAQTDMCGACHGLRTQLTSSSDAGGSFLQRFL
                     PELMTPDMFFRDGQQKGEVYKYASFLSSRMYAKGVVCSDCHQPHSGRLKLDGNALCMQ
                     CHTPSYDSPSHHFHPESSVGASCVNCHMPGKNYMVNDYRHDHSFRVPRPDQSAKYGTP
                     NTCNTCHADRTAGWAAEQINKWYGPERRWHFSDALLAAGQDPSDALPYLLELTGRDSV
                     PAIVRAQATMELNAAYGQQVIPTLQQLISDSSELVRMAAYNSLLHQPADRSLAMYQAG
                     WQDTVLAVRLAAYRATADIPVSGLSEVALKNKQSAGDEYLQFLMMNADMANGQVLFSE
                     YYQQRNDLEGAIDACEKALQIDDRLGEVRLNLAILYSRQGNMVAAVNHLKTFISYYPD
                     VSRGYYYLSLILNEQDRMTDAIQMIRKALSLDPYDTGMVANYVLMLYKSGQINEARAI
                     LQEALEAFPNDAGLQSLIAFIGIR"
     gene            complement(3799..4221)
                     /locus_tag="PK742_07115"
     CDS             complement(3799..4221)
                     /locus_tag="PK742_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76397.1"
                     /translation="MHTYLLTFLLALVCTGPEEPVFSIYPVDRWQVEQGVYNTDSAWL
                     SAGAPDLFPDMIPDFTSQTVIIFTEGGDCHMQVFTHPELAGDSLVVKVYNVYGGCRAG
                     GWETLMMLVNWTGPSEKIIFERILLDSLEEYKALYPDR"
     gene            complement(4260..5717)
                     /locus_tag="PK742_07120"
     CDS             complement(4260..5717)
                     /locus_tag="PK742_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU76398.1"
                     /translation="MRVLSMFAERGRGLLSLLAFCAYCLSLSAQEIPVQEYTIRELQE
                     DWDEMVELVLAVHPRATAFTDSAALYQEANRYREMLHDGLSTEQFHVLVRQWIRQIGC
                     GHTVARPGLDWYAAQKADSKLLPMEVYIQQDRIYIKSVYDTDSILVPGMEVIGINGHA
                     AEEILSAMYAIHPRDGYSASAAAAVSERLFRTYYLFLYGRSDEYMVTCIGKTSMTVSS
                     SLKGGDKPEEAVQPTDSLAKVILDADGIATLYLPAERTDIAVMDIDAFGFKHWKRFYR
                     SSFSYLEEQQINKLVIDLRNNGGGYFPNGNYLLRYLMPEDFDFTFSRPDRKLPGHPDL
                     TMKFSSRMTMRLFGLMPDHDKTDPWRDHRIGYSVKKKDHFDGQVWVLTNGNSFSLSSY
                     VASELQHKAGARTAGEETGGADIGSNAVLMTDLTLPHTGIRIAIPVYFLEHQVMPDET
                     GRGVMPDLLLSRDIRKEIDGNDSVLEGLLIRLQEK"
     gene            complement(5692..6135)
                     /locus_tag="PK742_07125"
     CDS             complement(5692..6135)
                     /locus_tag="PK742_07125"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU76399.1"
                     /translation="MSSQEYISSLPAERQEAVQALRDAFAKKLPKGFSESGGPTGIHF
                     AVPHSIYPNGYHVNPKQPLPFVSIISQKNFVAVYHMGLYADPETLSWFQQEYASRVPT
                     KLDMGKSCIRLKKIDQIPVGLFEELAQKFTTERWIGLYESAINVR"
     gene            complement(6213..6632)
                     /locus_tag="PK742_07130"
     CDS             complement(6213..6632)
                     /locus_tag="PK742_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQU76400.1"
                     /translation="MPVITIQTTVQKSMPLVWEAWTQPDHICNWNFASDDWCCPGATN
                     NLLPGGTFNYRMEATDGSAGFDFEGTYVEVEPHKLIRYTIGDGRLVDIRFEDGPGGIT
                     VTESFETEDVNSAELQRQGWQAILDNFKKVCGNIAMK"
     gene            complement(6636..7007)
                     /locus_tag="PK742_07135"
     CDS             complement(6636..7007)
                     /locus_tag="PK742_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQU76401.1"
                     /translation="MKLGAFSVSLNVKDLEASRAFYEKLGFAVFAGSADHKYYILKNG
                     NALIGIFEGMFSGNILTFNPGWDEEAQQVAPFDDVRKIQQHLKAEGLTLLSEADEQTE
                     GPASLMVSDPDGNVILIDQHI"
     gene            complement(7311..>8163)
                     /locus_tag="PK742_07140"
     CDS             complement(7311..>8163)
                     /locus_tag="PK742_07140"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HQU76402.1"
                     /translation="YMTPLPEFRQYAPFPEMYYENDSLSAEEVQAIFDSCHAAVRRFY
                     QDAGLEDFFRREAAPLYKKMMQELSGVRPPENYLSAFRSFYGNSADTYSIIVSVTSFN
                     GIGRSRKITLHAEDHIYQFVSADPSTQSANIDLDHTENITIGYTDPEYFREIAVHELI
                     HTAFERPLRYDSTNLLLIERLSFLYNAPMEKAMNAQGYYSWSQCLDEHLVRLGEYQLS
                     MLTGDTVQANRYLQYCISERGFIYLPVLQNLMEEYINNREMYPDIASFLPVLLDAWWR
                     SEQPEFD"
BASE COUNT         2120 a         2054 c         2011 g         1978 t
ORIGIN      
        1 gacgtgatct tcaagcccct ggacggcatg ggcggttccg ccatcttccg ggtcaaaccc
       61 ggggattcca acctcagcgt gatcctggag atgctgaccc tgcaacaaca actcagcgcc
      121 agatggcagg taaccattgt attgaagcgg gcttatacct gcatcaccac acctgatgga
      181 aaaatatata taaacggcag cggcaacccc ggtatggcca ccgcgggtag tggagatgtg
      241 ctgaccggca tcatagcagc ctggatggca caggggcttt caccaaccga tgcagccatt
      301 gcaggaacct atctgcacgg gcttgccggc gatcttgcta tgaaagaaca gggcgggctg
      361 aacatcatag ccggcgacat catcgatcat tacaattctg ccattaaaat tcttcgtgca
      421 tgaagatcgt ggtgctgagt ggtgcaggca tgagcgcaga aagtggcata gctacttttc
      481 gcggcgcagg cgggctttgg gaaggtcatc gggtggaaga tgtagccacc cctgaagcat
      541 ggcagaaaaa cccggagctg gtgttgcaat tctataacga gcgacgggaa aagatccgcc
      601 acgcacaacc caacaaaggc catacatatc tggccgaaat ggaaaaggaa catgaagtgt
      661 tcatcattac ccagaacatt gatgatcttc atgaacgtgc aggcagtaca caggtcttgc
      721 atctgcatgg tgagatcatg aaatccagga gtgaaaaata tacagaacct ttatatgatc
      781 agcccggaca tatttcaaca ggcgatctgt gtgaaagagg cacgcagctg aggccgcata
      841 ttgtctggtt tggtgaaccg gtaccaatga tggaacccgc catagcaatt gtttctacag
      901 cagatctgtt gctgataacc ggtaccagtc tggtggttta cccggccgct tccctgtggc
      961 attatgcccc tgctggctgt ccggtctatc tggtggaccc gaatccgccg gccggacttc
     1021 ctgctcatgt gcgcactatc agagaaacgg cagtcaacgg cattcaactt ttcagaaaga
     1081 tgatcagtca tggaacataa tccctggacc accctgcaac gggaagaaca ataccggaac
     1141 aactggatac aggtagaaga acatcaggtg ctgaacccgg caggcaatcc cggtatctat
     1201 ggtgtggttc gttttgccgc ttatgccatt ggtatcattc ctgttgatac ttttggaaac
     1261 acctggctgg ttgggcaata ccgctatccg ctggaagctt acagttggga aataccggaa
     1321 ggtggtggaa acaaaaacga tacacctgag cgatctgcac tgcgtgaact tcaggaagaa
     1381 accggagtca tggcaggcag gctggaactc cttgctcatc tgcataccag caatagtgtc
     1441 accgatgaag aagcattcat ctatctggca actgacctca cagaaggtca gtccagtccg
     1501 gaagaaacag aatcacttca gatcagaaag ctgccactta cagaagccat acaaatggca
     1561 atggacggtc gcatcacaga tgccatgagt gtggcaggtc tgctgaaagc caaacttgtt
     1621 ttaggtttgt aagatgcagc agaaactggt taaaagtatt ctgggagttg gagcattcct
     1681 tttcgtgtgc tggagttttg ttttctgtaa gcaacaagag accggcgctg ttacccctgt
     1741 agctgcagat gttgccggtt ttgtgggcga tcaggtgtgc ggatcctgcc atgcagaagc
     1801 ctatgcagac tggaaaggat ctcatcattt tcatgcgatg gaactgcctg acagcaacag
     1861 cgtgcgggga agatttgatg gtagcacttt tacggccgat ggtgtacggt accgtatgta
     1921 tcaacagaac ggtcagtacc tgtttgacat taccgaaaac ggacaggcaa cagaacaatt
     1981 tccggttgcc tatacatttg gatgggaacc attacagcaa tatctggtgg caagaccggg
     2041 aggacgatac cagacacttc gcatatcatg ggatacagaa aaacagcaat ggttccacca
     2101 gtatccggat accgtgatca cgcccggcga ctggctccac tggaccggtg ctgcgcaaac
     2161 atggaacagc atgtgtgcag cctgccacag cacccatctg gaaaagaatt atatcgctga
     2221 aaaagatagc tttcacacta cctggtccat tatcaatgta agctgtgaag cctgtcatgg
     2281 tcccggcgaa caacatgttg cactggcaac aaaaggcgac tggaggaacg gagctggtat
     2341 cagcaccttc agcaaagacg gcacacatgc ccaaacagat atgtgcgggg cctgccatgg
     2401 cttgcgcacc caactgacct cttcttcaga cgcaggcggt tcattcctgc agcgctttct
     2461 gcccgaactg atgacacccg atatgttctt ccgcgacggg caacagaaag gcgaagtgta
     2521 taagtatgcc tcttttctga gtagcaggat gtatgccaaa ggggtggttt gttcagactg
     2581 tcaccaaccg cattccgggc gcctcaaact ggacggcaat gccctttgta tgcaatgcca
     2641 tacgccatcc tatgacagcc cttcgcacca ctttcatcct gagtcatctg ttggtgcctc
     2701 ctgtgtgaac tgccatatgc cgggaaagaa ctatatggtg aacgactacc gccatgacca
     2761 cagctttcgt gttccgcggc ctgatcagtc ggcgaaatac ggcacaccca acacctgcaa
     2821 tacctgccat gcagacagaa ctgccgggtg ggctgcagaa cagatcaaca agtggtacgg
     2881 accagaaaga cgctggcatt tttcagatgc cttgctggcg gcaggacagg atccttcaga
     2941 cgccctaccc tatctgcttg aacttaccgg ccgagattcg gttccggcca tcgtccgtgc
     3001 acaggcaacc atggaactga acgctgcata cggccaacag gtcattccca ctttacaaca
     3061 gctcatcagc gacagcagtg aactggtgcg catggctgcg tacaacagtt tgctgcacca
     3121 gccagcagac agaagtctgg ccatgtatca ggcaggatgg caagataccg tgctggcggt
     3181 gagactggca gcatacagag ctacagccga tattccggtg agcggactgt ctgaagttgc
     3241 gctgaagaac aaacaatctg ccggcgacga atatcttcag tttctgatga tgaatgctga
     3301 catggccaac ggtcaggtac tgttcagcga atattaccag cagcgaaacg atctggaagg
     3361 cgccattgat gcctgtgaaa aagctttgca gattgacgac agactgggag aagtcagact
     3421 aaatctggcc attttgtaca gccggcaggg aaatatggtt gcagcggtca atcatctgaa
     3481 aacatttata tcctattatc ctgacgtaag cagggggtat tattacctgt cgctgatact
     3541 gaacgaacag gaccgaatga ccgatgccat tcagatgatc aggaaggctt tgtctcttga
     3601 tccgtatgac accggaatgg tggccaatta tgtgcttatg ctatataaga gcggacagat
     3661 caatgaagca agagctattt tacaggaagc tctggaagct ttcccgaatg atgccggact
     3721 gcaaagcctg atagcgttta tcggaataag ataacagacg caggttggca agcaaccggc
     3781 aatcacttcc ggttcatatc agcgatccgg gtatagtgct ttgtattctt ccagtgaatc
     3841 aagcagtatc cgttcaaaga tgatcttttc tgacggacca gtccaattga ccagcatcat
     3901 cagagtttcc catcctcctg ccctgcatcc accatacaca ttatacacct tgacaaccaa
     3961 ggaatctcct gccagttccg gatgggtgaa aacctgcata tgacaatcac caccttctgt
     4021 aaagatgatg accgtctgac tggtaaaatc cggtatcata tcaggaaaca gatcaggagc
     4081 accggcagaa agccaggcac tgtcggtatt gtacacaccc tgttccacct gccaccgatc
     4141 tacagggtaa atgctgaaaa ccggttcttc aggtcctgta cagaccagcg ccagcagaaa
     4201 ggtcaataag tatgtatgca tattcagagg atgctacaac aggaaatatt gcataagggt
     4261 cacttttcct gtaacctgat cagcaggccc tcgagtacag aatcattgcc gtcaatttcc
     4321 ttcctgatat ctcttgaaag caaaagatca ggcatgacac ctctgcctgt ttcatccggc
     4381 atgacctgat gttccaggaa atagacagga atggcaatgc gtatgccggt gtggggaagt
     4441 gtgaggtctg tcatcagaac tgcattggaa ccaatatctg caccgcctgt ctcctctccc
     4501 gctgtacgtg ctcctgcttt atgctgcaat tcagaagcca cataactgct gagggagaaa
     4561 ctgtttccgt tggtgagcac ccagacctgc ccgtcaaaat gatctttctt tttgaccgaa
     4621 tatccgatgc gatgatctct ccacggatca gttttgtcgt gatcaggcat cagaccaaag
     4681 aggcgcatgg tcatacgaga actaaacttc atggtcagat ccggatgacc aggaagcttt
     4741 ctgtcggggc ggctgaatgt aaaatcaaaa tcttccggca tgaggtaccg caacaggtag
     4801 ttaccgttgg gaaaataacc tcctccattg tttcgcaggt ctatgaccag tttgttgatc
     4861 tgttgttctt ccaggtagct gaaagaactg cggtagaatc ttttccagtg tttgaaacca
     4921 aatgcatcga tatccatcac tgctatgtcg gtacgttcgg caggaagata cagcgtagcg
     4981 ataccgtctg catccagaat gaccttcgca agactgtctg tcggctgtac agcttcctcc
     5041 ggtttatctc cgcccttcag actggaacta acggtcatgg aagttttccc gatacaggta
     5101 accatgtatt catcgctccg gccgtaaaga aacaggtaat aggtgcggaa aagtctctct
     5161 gaaactgccg ctgcggcaga tgcgctgtat ccgtcccgag ggtgtatggc atacatggct
     5221 gatagtatct cttctgccgc atgcccgttg ataccgatca cttccatacc cggcacgaga
     5281 atggaatcag tgtcataaac actctttata taaatcctat cctgttgtat ataaacttcc
     5341 atcggcagca gcttactgtc ggctttttgt gccgcatacc agtccagtcc tggtctggcc
     5401 acggtatgtc cgcaacctat ttgcctgatc cattgcctta ccagaacatg aaactgctct
     5461 gtggagagac catcgtgcaa catctcccgg taccggttgg cttcctggta caatgctgct
     5521 gagtctgtaa atgcggtagc cctggggtgc actgccagta ccagctccac catttcatcc
     5581 cagtcttcct gcagttcacg gatggtatat tcctgtaccg gaatttcctg ggcactcaat
     5641 gaaaggcagt atgcacaaaa cgccagcagg ctgagcaagc ctctgcctcg ttcagcgaac
     5701 attgatagca ctctcatata gaccgatcca tctttcggta gtgaattttt gtgccagctc
     5761 ttcgaacagc ccgaccggta tctgatctat tttcttcagg cggatacagc tcttccccat
     5821 gtccagtttg gtgggaaccc tgctggcata ttcctgctga aaccaggaca gggtttccgg
     5881 gtctgcatac agccccatat gatacacagc tacaaaattc ttctggctga taatgctgac
     5941 aaatggcagg ggttgtttgg gattgacgtg gtacccattc ggataaatac tatgcggaac
     6001 agcgaaatgt atgcctgttg gtccgccgga ttctgaaaac ccttttggca gttttttggc
     6061 aaatgcatca cgcagggcct gcacggcttc ctgtcgctct gccggtaatg aactgatgta
     6121 ttcctggctt gacatagggt caaatttaaa aaatcagaca cagtccggaa ctggaactaa
     6181 tagaacatat gcctgatgat gcgaacagtg ccttacttca tggcaatgtt tccgcatact
     6241 tttttaaaat tgtccaggat ggcctgccaa ccctggcgct gcagttctgc agagttgaca
     6301 tcttctgttt caaatgattc ggtcactgtg ataccacccg gcccatcttc gaaacgtata
     6361 tcaaccagcc ggccgtctcc gatggtgtag cgaatcaact tatgaggttc tacttccacg
     6421 taggtaccct caaaatcaaa accggcgctg ccatctgtgg cttccatccg ataattgaac
     6481 gtacctccgg gcagcaggtt gttggttgca cccgggcagc accagtcgtc agatgcaaaa
     6541 ttccagttac agatatgatc aggctgtgtc caggcctccc agacaagggg catgcttttc
     6601 tgaacagttg tttgaatggt tataacgggc atagcttaaa tgtgttgatc tatcagaatg
     6661 acattcccat ccgggtcgct gaccatcagg ctggccggac cttcagtttg ttcatcggct
     6721 tcagacagca acgtcagacc ttcagctttc aggtgctgct gaatcttgcg cacatcgtca
     6781 aacggtgcga cctgctgtgc ctcttcatcc caaccggggt tgaaggtcag gatatttcca
     6841 ctgaacatgc cttcaaaaat tccgatcagt gcattgccgt tcttcagaat gtaatacttg
     6901 tgatctgcac ttcctgcgaa tacggcaaaa ccgagttttt catagaatgc acgggaagcc
     6961 tccaggtctt tcacattgag gctgacagaa aaagctccga gtttcatgac gttggtttgt
     7021 attcaaacat agtcatttag cagcaacccc aaaaaaaagc accggccgaa accggtgctc
     7081 aagcagacat gcttaaccct ctgaatgtct gcgtccagca aaagtaaaac cttgcaaaca
     7141 atcttcctac tttctaaaga agtatttttc acatttattg tgtgaagtgt atccagaaag
     7201 caacagggcc gtttttgacg tataaaacaa ttgtaaatac ccctgccggc atgccccaca
     7261 ggcctttttt aattccggcg gcctgtgagc agataccggt tctcaggccg tcaatcgaac
     7321 tcaggctgtt cagatcgcca ccaggcatcc agtaaaacag gcagaaaaga ggcgatatcc
     7381 gggtacatct ctctgttatt tatatactcc tccatcaggt tttgcagaac aggcaggtag
     7441 atgaagcctc gttcgctgat gcagtattgc aggtatctgt tggcctgtac cgtatctccg
     7501 gtcagcatgg acagctgata ttcacccagt ctgaccaggt gttcatccag gcactggctc
     7561 caggaatagt atccctgtgc gttcatggcc ttttccattg gcgcattgta cagaaaagaa
     7621 agcctttcta tcagaaggag gttggtacta tcgtaccgca ggggccgctc aaaagccgta
     7681 tgtatgagtt catgaacagc aatttcacgg aaatattccg gatcggtata gcctatggtg
     7741 atgttttccg tatgatccag gtcaatgttt gctgactggg tggacggatc agcgctcaca
     7801 aactggtaga tatggtcttc cgcatggagg gtgatcttcc gcgacctgcc aatgccgttg
     7861 aaggatgtaa cggaaacaat tatggaatag gtatctgcac tgtttccata gaaagaacgg
     7921 aaggccgaca ggtaattctc aggtggccga acaccggata gttcctgcat cattttttta
     7981 tacaggggag ctgcctcacg gcggaagaaa tcttccagac cagcatcctg gtaaaaccga
     8041 cgcaccgcag catggcaact gtcaaagatg gcctgtactt cttcggccga cagagagtcg
     8101 ttctcataat acatctccgg aaagggagca tactgccgga attcaggaag tggggtcata
     8161 tac
//
LOCUS       DAOMAX010000111         8134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1298_length_8134_cov_4.718034, whole genome shotgun sequence.
ACCESSION   DAOMAX010000111 DAOMAX010000000
VERSION     DAOMAX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_8134_cov_4.718034"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK742_07145"
     CDS             complement(<1..249)
                     /locus_tag="PK742_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU76403.1"
                     /translation="MAWTDSDILQQFRDPRTREKAFTALTQQYSKRVYWHVRRMVEDH
                     EDANDITQNVLVKVWRKLDDFREEAGLFTWIYRIATNEA"
     gene            complement(276..1229)
                     /locus_tag="PK742_07150"
     CDS             complement(276..1229)
                     /locus_tag="PK742_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HQU76404.1"
                     /translation="MIKIDYSNQKDTRSGFGAGMVELGKTNPDVVALCADLIGSLKLD
                     KFIENHPERFFQCGIAEANMIGIAAGLTIGGKIPFASTFANFATGRVYDQIRQSVAYS
                     GKNVKIAASHAGLTLGEDGATHQILEDIGLMQMLPGFTVINPCDYNQTKAATIAAANH
                     QGPVYLRFGRPKWPVFTPEDQEFVIGKAWKISEGKDVSIFATGHMVVKAIEAANQLEE
                     QGISAEVINIHTIKPLDREAVIESVRKTGCAVTAEEHQRLGGLGSAIAQVLAEEHPVP
                     LSFVAVQDSFGESGTTEQLLTKYGLQASNIVEAARSVISRK"
     gene            complement(1242..2618)
                     /locus_tag="PK742_07155"
     CDS             complement(1242..2618)
                     /locus_tag="PK742_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQU76405.1"
                     /translation="MRRTIHIIVVLLVLTGLKPMAVKAQAAEEVYTTRMLVIFDASRS
                     MLATWGTSTRWTEAKKILTELSDSVNMMPHVETALRVYGHQTVQMENDCYDSKLEVPF
                     RNQNAVRFKNTVNYLRPQGVTPIAYSLEQSIKDFGEPKPNQRNVIVLITDGAESCGSN
                     PCEVFRQLREKGVVMKSYVIGMGIEEEDFASLECMGEFMNIENPSEVDAILDLTLKKV
                     FNSTTVRVDLLDQQKKATETDVPMTFYNKTSHKPVYQFYHTINPKGQPDTMAVDPNQQ
                     YDITFHTYPPQVQENVSLTPFVYNVIQQPSAQGQLEVTVRGDSYKNRINCIVSRDGDY
                     VHVQNSGESVRYLLGEYKLEILTLPVITADRIKIEQNKTTIIEIPAPGTITFQKAAEL
                     YGGVYMPDGDRWVEVYELASNNLRESLALQPGTYKVIYRFKNSKAMTSTMEKLFEVNS
                     GTSVTLRL"
     gene            complement(2621..3976)
                     /locus_tag="PK742_07160"
     CDS             complement(2621..3976)
                     /locus_tag="PK742_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQU76406.1"
                     /translation="MKRFLLLLFIWPALAGFGQNSSEDTRHILILLDGSGSMMDTWKG
                     TTKWTTAKRLIGETLDSIQRENPDVEIGLRVFGHQSPRAMKDCRDSKLEVAIGRNTAQ
                     AIITKLGEITPQGNTPIAYSLFLASGDFTAEGINSIILITDGIENCEGDPCASAESLS
                     SKRISLKPFIIGIGLNEADKHMFDCVGSYYDAADSIQFKNAMNIVVSQALNNTTAQIN
                     LLDAYGRANQTNVELTLYDSYSKEVRYHFVHSLNAKGEPDTLFLDPLGRYDIVAHTTP
                     AVTRKNVELNPGRHNIIGLEAPQGVLKLYEKGQSGFSGKQCVVRDPETHEIVYVQNLN
                     TEQLYLSGKYDLEVLTLPPIFYHNYFIQGNYVNQIDIPQSGVLSLNTNDNVQVSVLYD
                     DQGNWVKVWEGSLNREISNVDLQPGEYLLVYRSNIRKTADNTKQTAVSIRSGRTFSVK
                     L"
     gene            complement(4077..4397)
                     /locus_tag="PK742_07165"
     CDS             complement(4077..4397)
                     /locus_tag="PK742_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76407.1"
                     /translation="MSLLISACAVRPAQPTEADVLRATEMYPDITLDQLNQGLDYYKQ
                     YCTPCHKLKDPNSETVADWQKIVPAMAKKAGRAEDIADIPEDVQLSITRYLTTMTIRT
                     VSAQ"
     gene            complement(4480..6057)
                     /locus_tag="PK742_07170"
     CDS             complement(4480..6057)
                     /locus_tag="PK742_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76408.1"
                     /translation="MAGCILASVNATGQTTTYDVDPDIVLDNHLWFYHTDYFEWDADQ
                     DGTADFRLGLDFNSSSYSSAYIQPLNDAQIVRDIVELVKICDGVTVADTLEVPLVHVG
                     IAGEYISSESAFDTLQTKVFGSFGGPSEDPDCYFPLAGEKTLLIRNIPVAISMPDGLH
                     YGWLKTSLYESGQHRYWRLYEAVLSDMPDEPVTIPDNEVTPPVWINAVDISDYNDAAD
                     LSGSFRPTDIPFAIAEHRVIVVPVSVSDPIDIASLLALPADRYQIVLPSEADSLHFQF
                     DHLKKDLEGNLIHNNTPYRLLVISIPDIATGLDPVLSVYSEEITLRTWIEGATVFLSD
                     DGDDFSPSDFRVQFPPISPETLVSQYRIMLVKSDQLDSWTLEKALDVAESNVLYAAPD
                     NSDHDIHLPDGFMDVDGDMITPWQTYVACLQVVASEVAASDTLAFSAPAMLSHPFSNT
                     DGFHLIQSNGQVMLIAPSDYASGYVLNMYNAAGELVWQKESNEAFLQITYDGRNAVYL
                     LQTIKGSETTTFKTFLH"
     gene            complement(6130..6975)
                     /locus_tag="PK742_07175"
     CDS             complement(6130..6975)
                     /locus_tag="PK742_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQU76409.1"
                     /translation="MADIQQLERICSQVRRDIIRETHACQSGHPGGSLGCTEFFVALY
                     FEVMQHSPDFSMDGQGEDLFFLSNGHISPVWYSVLARSGYFPVSELATFRRLDSRLQG
                     HPATHEHLPGIRVASGSLGQGMSVAIGAALTKKLNGDDHLVYSLHGDGELDEGQIWEA
                     AMFAAHHKVDNLISTVDLNGQQIDGPTEKVMSLGNVRAKWEAFGWDVIECADGNNMAQ
                     LLASLADAKTRTGKGKPVMILMHTAMGKGVDFMEGTHHWHGVAPNDEQKDKALAQLEE
                     TLGDY"
     gene            complement(7024..>8134)
                     /locus_tag="PK742_07180"
     CDS             complement(7024..>8134)
                     /locus_tag="PK742_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQU76410.1"
                     /translation="KPGSRPFCLIDYFPDDFLLVIDESHVTVPQIGAMYGGDRARKTN
                     LVEFGFRLPSALDNRPLNFDEFQQLAGQTIYVSATPGDYELEQTGGVIVEQVVRPTGL
                     LDPPIEVRPSINQIDDLLDEVDERIKKGERVLATTLTKRMAEELTKYLQKVNIKCRYI
                     HSEVDTLERVEILRDLRLGKIDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRNTRS
                     LTQTAGRAARNVNGLVIMYADKVTESMQRTIDETERRRAKQLSYNAEHGIVPRTVVKS
                     TEEIFKQTSVLEIRGNAPRPYVEDEVVIAAADPVVEYMDKQQLEKAIAASRKKMLAAA
                     KDMDFLEAARLRDEMFGLQKLLDEKFSSAGDDVDK"
BASE COUNT         1886 a         2069 c         1980 g         2199 t
ORIGIN      
        1 tgcctcattt gtggcgatgc gatagatcca ggtaaagaga ccagcttctt cgcgaaagtc
       61 gtccagtttt cgccagacct ttaccaatac attctgggtg atgtcgttcg catcttcatg
      121 gtcttccacc atgcgacgca catgccagta aactcttttg gaatactgtt gcgtgagggc
      181 tgtaaaagcc ttctcgcggg ttcggggatc cctgaactgt tgtagtatgt cgctgtcggt
      241 ccatgccatc cggccggggt ttcgacaggc tcaggttact tgcgggagat gacggaacgg
      301 gcagcttcca cgatgttcga tgcctgtagt ccgtacttgg tgagcaactg ttctgtggtg
      361 ccactttcgc cgaaactgtc ctgaacagct acgaatgaaa ggggaaccgg atgctcttct
      421 gcaagtacct gcgcaattgc actgcctaat ccgccaagtc gctgatgctc ctcagccgtg
      481 acagcacagc ctgtcttgcg cacgctttct atgacggcct cacggtccaa aggtttaatc
      541 gtatggatat tgatgacctc tgcagagata ccctgctctt ccagctgatt ggctgcttct
      601 atggccttga ccaccatgtg tccggtggcg aagatgctca cgtcttttcc ctcactgatc
      661 ttccaggctt tgccgatcac gaactcctgg tcttccgggg tgaataccgg ccatttgggt
      721 cggccgaaac gcagatatac cggaccctga tgattggccg ctgcaatggt ggctgccttg
      781 gtctggttat agtcgcaggg attgataacg gtaaacccgg gcagcatctg catcagaccg
      841 atgtcttcca gtatctggtg ggtagcacca tcctctccaa gggtcagacc tgcgtgagat
      901 gctgcgatct tgacattctt tcccgaatag gccacgctct gacggatctg gtcatagacc
      961 cttccggtgg cgaagttggc aaaggtggat gcaaaaggga tctttccgcc gatggtaaga
     1021 ccggcagcga taccgatcat attcgcttct gcgataccgc attgaaaaaa tctttccgga
     1081 tggttttcga tgaatttatc gagcttgagc gatccgatga ggtcggcaca caaggccact
     1141 acatcgggat tcgttttacc cagttctacc atgcccgcac caaatccgga gcgggtgtct
     1201 ttctgattgg aatagtcgat cttgatcaag gttcagtgtt ttcagagtct cagtgtcaca
     1261 ctggtaccgc tattaacttc gaacagtttt tccatggtgg aggtcatggc cttgctgttc
     1321 ttgaaccggt agatcacctt gtaggtacct ggttgtaagg ccagagattc acgcaggtta
     1381 ttgcttgcca gttcgtacac ttccacccat cggtcgccgt ccggcatata cactcctccg
     1441 tacaactctg ctgccttttg gaaagtaatg gttccggggg caggtatttc gatgatggtg
     1501 gtcttgttct gttcgatctt aatgcgatcg gcagtgatga caggcaaagt taaaatttcg
     1561 agcttgtatt ccccaagcag gtaacgcaca gactctccgc tgttttgcac atggacatag
     1621 tctccgtctc tgcttacgat gcagttgatg cggttcttgt aactatcgcc ccgaacggtc
     1681 acctctaatt gtccctgtgc cgatggttgc tggatgacat tatagacaaa tggtgtcaga
     1741 gagacattct cctgcacctg tggcggatag gtgtggaaag taatgtcata ctgctggttc
     1801 ggatccacgg ccatggtatc cggttgtcct ttcggattga tggtatggta gaactgatag
     1861 acgggcttat gcgatgtctt gttgtaaaaa gtcatcggca catccgtttc cgttgccttt
     1921 ttctgctgat ccaggagatc aactcttacc gtcgtggagt tgaatacttt cttcagggtg
     1981 agatcgagaa tagcatccac ttctgatgga ttctcgatgt tcatgaattc tcccatgcac
     2041 tccagagaag cgaaatcttc ttcttctatt cccatgccga tcacatagga tttcatcact
     2101 acgccctttt cccgcagctg ccggaatacc tcacagggat tggatccgca ggattctgca
     2161 ccatcggtga tgagtacgat cacattgcgc tgattgggtt ttggctcacc aaagtctttg
     2221 atgctctgct ccagtgaata ggcaatggga gtgactccct gcgggcgaag gtagttgacg
     2281 gtattcttga aacgcacggc attctggttg cggaagggca cttccagttt ggagtcgtaa
     2341 caatcgttct ccatctgtac cgtctgatga ccataaacac gcaaagctgt ttccacatgc
     2401 ggcatcatat taacgctgtc agacagttcg gtcaggatct ttttggcttc cgtccagcgg
     2461 gtacttgttc cccaggtagc aagcatactc ctgctggcgt cgaagatgac aagcatgcgg
     2521 gtagtataga cctcttctgc agcctgagct tttacagcca taggtttcag tccggtcagc
     2581 accagcaaaa caactatgat atgaatggtc ctgcgcatgc ttatagtttc acactgaatg
     2641 ttcttccgct tctgatagat accgccgttt gtttcgtatt gtctgcggtc ttcctgatgt
     2701 tggaacgata taccagcaga tattcacccg gttgcagatc cacgttgctg atctcgcggt
     2761 tgagtgatcc ttcccatacc ttgacccagt tgccctggtc atcgtacaac acactgacct
     2821 ggacgttatc atttgtattc agactcagca caccactttg cggaatgtct atctggttga
     2881 catagttgcc ctgaatgaaa tagttatggt agaaaatggg gggaagggtc agcacttcca
     2941 gatcatattt tccgctcaga tacaactgtt ctgtattgag attctgtaca tacacgatct
     3001 cgtgggtctc cggatcacgc accacgcatt gcttgccgct gaaacctgac tgccctttct
     3061 catacagttt gagcactccc tgaggtgctt cgagtccgat gatattatgc cgacccggat
     3121 tcagttctac attctttctg gtgaccgccg gagtggtgtg ggccacgatg tcatatctgc
     3181 ccagcggatc caggaacagg gtatccggtt ctccctttgc attcaggctg tgcacgaaat
     3241 gatagcgcac ttccttgctg tagctgtcat aaagtgtcag ttccacgttg gtctgattcg
     3301 cccgtccata agcatcaagc aggttgatct gggcggtggt attgttgaga gcctgcgaca
     3361 cgacaatgtt catggcattt ttgaactgaa tagaatcagc agcatcataa taggagccca
     3421 cacaatcgaa catgtgttta tcggcctcat tcagtccgat accaataatg aaaggtttta
     3481 gtgagatgcg tttgctgctg agtgattcgg cgctggcgca tggatctcct tcacaattct
     3541 caataccatc tgtgataagg atgatggagt tgatgccctc agcggtgaaa tcgccggaag
     3601 caaggaagag gctgtaagcg atgggggtat ttccctgagg ggtgatctct cccagtttgg
     3661 tgatgatggc ctgagcggta ttcctgccga tggccacttc cagtttagaa tcgcggcagt
     3721 ctttcatggc ccgcggactc tgatgtccaa acacccgaag gccgatctct acatccggat
     3781 tctcccgctg tatgctgtcc agtgtctctc caatgagccg cttcgcagtg gtccatttgg
     3841 tggttccctt ccaggtatcc atcatagagc cgctaccatc cagcaaaatg aggatgtggc
     3901 gggtatcttc agacgaattt tgcccgaaac cggccagtgc aggccagatg aacagcaaca
     3961 ggagaaacct cttcaattgg agatagttac gtaactaacg caatagtacc catattttgt
     4021 accaccactg tcatgctttc aacaatgatt gtgcctaata gtggacgctg tggcaatcat
     4081 tgcgccgaaa ctgtccggat tgtcatggtg gtgaggtaac gggttattga caactgaaca
     4141 tcttccggaa tgtctgctat atcttcagcc cttcctgcct ttttagccat agccggtacg
     4201 atcttctgcc agtcggctac ggtctcgctg tttgggtcct tgagtttatg acagggtgta
     4261 cagtactgtt tgtagtagtc caggccctga ttcagctggt ccagtgtgat atcagggtac
     4321 atttctgttg cacgaagcac atcggcttct gtgggctggg caggcctgac cgcacaggct
     4381 gatatcaaca aagacattcc aagtgtataa aagattgagt acgggttgtt cattatacgg
     4441 ttttggtgaa tctgaagtac agatattgta ccagaataat taatgcagaa aagtcttgaa
     4501 agttgtggtc tcagaccctt tgatcgtctg tagcagatac acagcgtttc tgccatcgta
     4561 ggtgatctgc agaaaagcct cattgctttc cttttgccag acaagttctc cggctgcatt
     4621 atacatgttt aagacatagc cgcttgcata atctgaaggc gcaatcagca tcacctgccc
     4681 gttggattga atcagatgaa atccatcagt attgctaaat ggatggctca gcatagccgg
     4741 tgcactgaat gcgagtgtat ctgatgcagc cacttcactg gcaaccacct gaaggcatgc
     4801 cacataggtt tgccagggtg tgatcatgtc tccatccaca tccataaacc catccggaag
     4861 gtgaatatcg tgatcactat tgtcaggtgc cgcataaagt acattagatt ctgcaacatc
     4921 taaggctttt tccagcgtcc aactatcaag ctgatctgat ttaacaagca ttatcctgta
     4981 ttgactgacc aaagtttcag ggctaatggg tggaaactga accctgaaat cagatggaga
     5041 aaaatcatct ccatcgtctg acaggaaaac cgtagcgcct tctatccagg tacgcagggt
     5101 gatctcttct gaataaacag acaagaccgg gtccaatcct gttgctatat cggggatact
     5161 gattacaagt aacctatatg gtgtgttgtt atggatcagg ttgccttcca gatccttttt
     5221 taaatgatca aactggaaat gaagtgaatc tgcctctgat ggcaaaacaa tttgataccg
     5281 gtctgccggc aaggcaagca gggaggctat atctatcggg tcagacacag aaacaggaac
     5341 aacaatgacc cgatgctctg cgattgcgaa cggaatatcc gtaggtcgaa aagagcctga
     5401 cagatctgcg gcatcattat aatcactgat atccactgca ttgatccaaa caggaggagt
     5461 tacttcatta tcaggtatgg taaccggttc atctggcata tctgacagaa cagcttcata
     5521 tagtctccag taacgatgct gcccggattc atacagcgag gtcttcagcc aaccatagtg
     5581 gagtccgtcc ggcatactga ttgcaacagg aatatttctg atcagcaagg tcttttcacc
     5641 ggccaacgga aaatagcaat ccggatcttc agaggggcct ccaaaagagc caaatacctt
     5701 ggtctgcaag gtgtcgaacg ccgattctga actgatatac tctcctgcaa taccaacatg
     5761 cactagcggt acttcaaggg tgtcagccac cgtgactcca tcacagatct tgaccagctc
     5821 cacaatatct ctgactattt gagcatcgtt cagaggttgg atataagcag atgaatagga
     5881 actggagttg aaatcaagac ctaatctgaa atctgccgtt ccatcctgat ctgcatccca
     5941 ctcaaaataa tctgtatggt aaaaccagag atggttatcc agcactatat caggatcgac
     6001 atcatatgtg gttgtctgac ccgttgcatt aacagatgca aggatacatc cagccattcc
     6061 ggtatagacg ggccacgtta aagtcagatg cttccgtgta ctgaccatcc tcaaatatag
     6121 gaaattagtt caatagtcgc cgagggtctc ttccagctga gccagagcct tatccttctg
     6181 ctcatcattc ggcgcaacgc catgccagtg atgggtgccc tccatgaagt ccactccttt
     6241 tcccatggca gtatgcatca ggatcatgac aggcttgcct tttccggttc tggtctttgc
     6301 atcagccaaa gatgcaagca attgagccat attgttacca tcggcacact cgatcacatc
     6361 ccagccaaat gcttcccact tggcacggac atttccgagc gacatgacct tttctgttgg
     6421 tccatctatc tgctgcccgt tcaggtcaac agtagagatc aggttatcca ctttgtggtg
     6481 ggcagcgaac atagctgctt cccagatctg gccttcatcc agttctccat cgccgtgcag
     6541 agaatacacc aggtgatcat cgccattcag tttcttggta agtgcagccc cgatggccac
     6601 gctcatgccc tgacccagtg aaccactcgc tacacgaata cctggcaggt gttcatgcgt
     6661 ggcgggatgg ccctgcaacc tggaatccag gcggcggaaa gtagccagtt cagaaacggg
     6721 aaaatagcca cttctggcca gcacactata ccacaccggg ctgatgtgcc cgttgctcag
     6781 gaagaacagg tcttcaccct gtccgtccat cgaaaaatcc gggctgtgtt gcatgacctc
     6841 aaagtacagt gctacgaaga attccgtaca acccagagag ccgcccgggt gcccgctctg
     6901 acaggcatgg gtctcacgaa tgatgtctct tctgacctgg gaacaaatgc gctcgagttg
     6961 ttgaatgtct gccatgtttg ggatttgagg taaaaataag gtgattgaag aattgggagc
     7021 gggtcattta tctacatcat caccagctga gctgaatttt tcatccagaa gcttctgcaa
     7081 accgaacatt tcgtcccgca atctcgctgc ttccaggaaa tccatgtctt tggctgctgc
     7141 cagcattttc tttctggaag ctgcaatggc tttttccagc tgttgtttgt ccatgtattc
     7201 caccacaggg tctgctgctg cgatgaccac ctcatcttcc acatagggcc ttggtgcatt
     7261 gcctctgatc tccagtacgg atgtctgctt gaagatctct tcggtcgatt tcactaccgt
     7321 tctcggaacg atgccatgtt cggcattata ggaaagttgt ttggcacgtc gtcgttcagt
     7381 ttcatcaatg gttcgctgca tgctttcggt gaccttgtcg gcatacatga tgaccaggcc
     7441 gttgacattt cgtgccgccc tgccagctgt ctgggtgagt gacctggtat tgcgcagaaa
     7501 tccttccttg tcggcatcca gtatggccac cagcgatact tccggcaggt ccagtccttc
     7561 ccgcaacagg ttcacgccca ccagcacatc gatctttcca agacgcagat cacgcagtat
     7621 ctccacacgt tccagggtat ccacctcact gtggatgtaa cgacacttta tatttacttt
     7681 ttgcagatat tttgtgagct cttctgccat gcgctttgtg agcgtagtgg ccagcacccg
     7741 ttcgcctttt ttgatgcgct cgtccacttc gtccagcagg tcgtctatct ggttgatact
     7801 gggtctgacc tcaatgggcg gatccagcaa tcctgtcgga cgcaccacct gttccacgat
     7861 gacaccaccg gtctgttcca gttcataatc gcccggagtg gcactcacat aaatggtctg
     7921 acctgccagt tgctgaaact catcaaagtt cagcggtctg ttgtccaaag cagacgggag
     7981 tctgaatccg aattccacca ggttggtctt gcgggcacgg tcgccgccat acatggctcc
     8041 gatctgggga acggtcacgt ggctttcatc aatgaccagc agaaaatcat ccgggaaata
     8101 atcgatcagg cagaacgggc ggctaccggg tttt
//
LOCUS       DAOMAX010000112         8032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1333_length_8032_cov_4.231415, whole genome shotgun sequence.
ACCESSION   DAOMAX010000112 DAOMAX010000000
VERSION     DAOMAX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1333_length_8032_cov_4.231415"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..2067)
                     /locus_tag="PK742_07185"
     CDS             complement(163..2067)
                     /locus_tag="PK742_07185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HQU76411.1"
                     /translation="MTALDILQKYWKYPAFRPGQEDIITAAVAGRDVLALLPTGGGKS
                     ICFQVPGMLHHGTTLVISPLIALMTDQVNQLKDRGIEAEAIHAGLPWKTAERILDNTA
                     SGAVKFLYVSPERLVSERFRDRLPYLQIKLLVVDEAHCISQWGYDFRPPYLRIAEARE
                     LLPGVPVMALTATATEAVAQDMKKLLQMRQPYVYRSTFLRSNMHLIVREEENKFQKLA
                     EIMDAVKGPAMVYVRSRKGAREVAQFLQRRRIAAAYYHAGLSADNRSRIQQEWISGKL
                     RAVACTNAFGMGIDKPDVRLVLHWDLPDSPEAYYQEAGRAGRDGNACYAGIIYQQQDL
                     DRIRESVLGQYPDPEQVRQVYHAVAASVQLAAGAGPGASFPFDRDKITSFTGLLPLTV
                     NNALQVLQQQGFLYLNEAARNVSVIRVLVDNETLYRYQVENRSIEPVIKALLRSCPGL
                     FEEHVMVRESEIAHFTGKRTEDVVKALRFLQEQEVMDYQPSGDDPQVVFLTERYNAAD
                     LPLDTGLMKKIREQAALRLQAMMDYVKGKPGCRMQKILAYFGEASSKCGMCDHCVEAD
                     KHALSDQDILAVYDWSRTLLVSGPVTVQILMEQKPPLKKEKTLEAISYLVEHKWLRYN
                     EADQLEWVNA"
     gene            complement(2075..3004)
                     /locus_tag="PK742_07190"
     CDS             complement(2075..3004)
                     /locus_tag="PK742_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU76412.1"
                     /translation="MKILITGGAGYIGFNLAKALCRLTEVTQVSLYDNFSRHNGSIFF
                     DPVVGANKIELITADILDTRKLRKAMAGKDLVVHLAARVTTPFANQDPHFFEQVNHWG
                     TAEVVYAAEDLQINRLIYLSSTAVYGRTTGMVDESTAAQPQTYYGISKRRGEDHVQRL
                     TEKGRAHIIRCGNVYGYSPALRFDAVINHFMMQANTEKRLSIHGTGMQVRSFVHIHEV
                     VEMLSQLSVKEVPSGVYNFVTKSLRINEIADAIEDLYPGLERIYINQHIGMSSLEVSP
                     ETALHQYLTLTGTTLKEELRNFRDHFTFGHSIQ"
     gene            complement(3001..3669)
                     /locus_tag="PK742_07195"
     CDS             complement(3001..3669)
                     /locus_tag="PK742_07195"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQU76413.1"
                     /translation="MPSFEQIAQQIQSYQEAGKTLFLSSSFQTHSIPLLHMISRIDDS
                     IPVYFLQTGYHFPETIRFRDEVASQLGIQIRSVASTTPRIMQLNEKGNLLYTSDPDYC
                     CYLNKVAPMEPLLDQFDVWINGVRGDQNDNRASFTVEQPTHRKAVRYHPVIDWTQKMI
                     WDYIAQFDLPTHPLDMQGYVSIGCEPCTRRADLALLSHERGGRWFGMQKTECGLHTEL
                     IEKK"
     gene            complement(3669..4394)
                     /locus_tag="PK742_07200"
     CDS             complement(3669..4394)
                     /locus_tag="PK742_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQU76414.1"
                     /translation="MEVDIFIPCFIDQLSPDTGFNMVKVLEHVGCKVHYNPGQTCCGQ
                     PAFNAGQWEKMDDVGKKFITDFSAVDRTIVSPSGSCTGFVRNFYNELYANTPYHNYYK
                     QVKRNLFEISEFLVDVMQQTDLGGKFQAKVTYHDACGALRECGIKEQPRKLLAAVEGL
                     ELVEMDFSETCCGFGGTFAAKFEAISSGMADNKGQFAAATGAEYVVSTDYSCLMHLQA
                     YFDKQQIPVKCIHLIDLLAKSIS"
     gene            complement(4437..5870)
                     /locus_tag="PK742_07205"
     CDS             complement(4437..5870)
                     /locus_tag="PK742_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76415.1"
                     /translation="MKQLLRSFPMRLPLLFLLLLTFRNEAFAHVKWFTDGSYADKPLS
                     VPEVVSNVFIWLLGLALVVISVGVWLDRQIASSKWYRDLDGWFESKRSSSVMVMRIAA
                     GMLFLLSWQGGAMLAPTLMVPSKYEWVGMFQFLLAFLLLFDKTVPYAGIGTIILYLLG
                     CVIYDPFHMLDYALFTGVGYYLATSSSRHVKLKQSGLTVLYLTVGFSLCWVALEKFIY
                     KDWSMFLVTEHPQLAMGLNYDFFISSAAFVEFSLGYLLIVNLLQRPLAVLITIVFFLT
                     TAVFGKVEVIGHTLVHGALVVFLLEGPGDVYHFLHRKFHTIGKRILFTTSGFLVLLFS
                     LLFAYHLLANNKYERKQKFLSNKPTTHMHGQIELAGYPPDELPDVRMEINDDPMGGYN
                     IRVTTTNFKFTPENVNTSNRMYEGHAHLHINGEKVARIYGEYYHLAAMPPGTYEISVT
                     LNSNNHDDYVIRGNPIEDTKILVVKGN"
     gene            6023..>8032
                     /locus_tag="PK742_07210"
     CDS             6023..>8032
                     /locus_tag="PK742_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU76416.1"
                     /translation="MKSLKIKIILWFVLGISLAGAGIWVVYDSFQQLLAAVQVLTAPD
                     KAYEAADQIRTDLVITENSMRAYLMSGDNEELDTYLEGMDRIELYLDTLPQIQVKGMD
                     DDDIDEIVTLLQTRADLMYDLVELKTDQREQSLAAKTLGQFQDQLAARNRQARDQPST
                     IQTDSVPGLKASNASQPADEEKEKKRLLNLFSKKSREKDNTTVQADSIAESVPEETQK
                     TRLSATGVQYSSDLDMEQVRKILRDMGREEAVSNALLDERELEILASDRQLMEKVNAI
                     MDRIEKRNAEYQAAVINQANQQVTDTSVQLFRAVIGAILAGLILLTVLMIDINRMHRY
                     RTQLEESRDYAQRLATARQRFLASMSHEIRTPLNAIIGFSEQLQQNGTSETKEQADFI
                     HSASGHLLEIVNDILDYSKLEFGKASLHQAPFTMQEVLDEVHGILKAKAEEKQLTWSL
                     ELDNGCRKWVEGDALRLKQVLINLAGNAIKFTSSGSVTIKVGTVERDRFTDFRILVID
                     TGIGIDKNAQARIFDDFSQADSSTSRSYGGTGLGLAICKKIIEQHHGTIRVESEPGSG
                     TTFIVELSYRNTKEAATEKKTSRAGRMELKGMKILVADDELFNIRLATMVLEKAGATV
                     TSCQQSDDMESCLRVRYDAYLIDLHMPGRDGVSVAHAIRTKHPDAR"
BASE COUNT         2162 a         1966 c         1901 g         2003 t
ORIGIN      
        1 aaaacgggtt caatctgacg gttgcatact tattctgaac cagcggaacc gatctttttt
       61 tggtaaaacc cacgagagtc acctggtatc cggcatcctg caaggtagta catatacgat
      121 gcatgcgctg atcgtaagtc aggtcattgg tgacagtaaa gactatgcgt ttacccattc
      181 cagttgatct gcttcgttat accgcaacca tttatgttcc accaggtaac taatggcttc
      241 cagggtcttt tctttcttta gcggaggctt ttgctccatc aatatttgca ccgttacggg
      301 tccgcttacg agcagggttc tggaccagtc atataccgcc agtatatcct ggtcagacaa
      361 ggcatgttta tcagcttcca cgcaatgatc gcacatgcca catttgctgc ttgcttcgcc
      421 aaagtacgca agtattttct gcatcctgca accaggcttc ccttttacat agtccatcat
      481 ggcctgcagg cgaagagctg cctgttcccg gatcttcttc atcaggcctg tatccagcgg
      541 aagatcagca gcgttatatc gttcagtcag aaagaccacc tgcggatcat cgccgcttgg
      601 ctggtaatcc atcacttcct gttcctgcag aaaccgaagg gccttcacca catcttctgt
      661 tcttttgcct gtaaagtgtg caatttcaga ttctctgacc attacatgct cttcaaacaa
      721 tcccggacag gaacgcaaca aagctttgat aaccggttct atgctgcggt tttctacctg
      781 gtatctgtac agggtctcat tatcaacaag caccctgata acagacacgt ttctggcagc
      841 ttcattgagg tacagaaatc cttgttgctg cagcacttgt aaagcgttgt tgaccgtgag
      901 cggcaacagt ccggtgaaag aagtgatctt gtcgcgatca aacggaaagg aagcccccgg
      961 gccagcaccg gctgccaact ggacacttgc agccactgca tgatagacct gtctcacctg
     1021 ttcggggtcg ggatactggc ctaacacact ttcgcggata cggtccaggt cctgctgctg
     1081 ataaatgatg ccggcataac aagcattgcc atctcttcct gctcttccgg cttcctgata
     1141 gtatgcttcg ggcgaatccg gaaggtccca atgaagcacc aggcgcacat caggtttatc
     1201 tatgcccata ccgaatgcat tggtacaggc cactgcacgt agttttccgg atatccattc
     1261 ctgctggatc cggcttcggt tatcagcaga caagccggca tggtaatagg ctgctgctat
     1321 ccttcttctt tgcaggaact gcgccacttc ccttgcacct ttgcggcttc gcacatatac
     1381 catggcagga cctttcactg catccatgat ctcagcgagc ttttgaaatt tgttctcttc
     1441 ttcgcggaca ataaggtgca tatttgaacg caggaaggtt gaccggtaaa catatggttg
     1501 ccgcatctgc agcaattttt tcatgtcttg tgccacagct tccgttgcag tggctgtaag
     1561 tgccatgacc ggcacacccg gtaacagttc tcttgcttct gcaatgcgca ggtaaggagg
     1621 tctgaagtca tatccccatt gagagatgca gtgggcttca tctaccacaa gcagttttat
     1681 ctgcagataa ggcaatcgat ctctgaaccg ttcactaaca agcctctccg gagacacata
     1741 cagaaatttc acagcaccgc tggcagtatt gtccagtatt cgttctgctg tcttccacgg
     1801 caaacctgca tgaatggcct ctgcttcaat tcctctgtct ttaagttggt taacctggtc
     1861 cgtcataagg gctataagcg gagagatcac caatgtagtt ccatggtgca gcattcccgg
     1921 cacctgaaaa caaatcgatt taccgccacc agttggaaga agcgccagta catcacgacc
     1981 ggcaaccgct gctgtaataa tatcttcctg acccggacgg aaggccggat atttccagta
     2041 tttctgcaga atatccaatg cggtcacagg ccgcttattg aatagaatga ccaaatgtga
     2101 aatgatcgcg gaagttgcga agttcttctt tcagcgttgt accggtaagt gtcaggtact
     2161 gatgcagtgc tgtttcaggt gacacttcca ggctgctcat gccgatatgc tgattgatgt
     2221 agatccgttc cagtccggga taaaggtctt cgatcgcatc agctatttca ttaatgcgaa
     2281 gagacttggt tacaaaattg tacacaccgg aaggcacttc ttttacactg agctgtgaca
     2341 gcatctccac cacttcatga atatgcacaa aggatcgtac ctgcatacct gtaccatgaa
     2401 tggataatct tttttcagtg ttggcctgca tcatgaaatg attgataact gcatcaaatc
     2461 gcaaagccgg gctgtagcca tacacattcc cgcagcggat tatatgtgct ctcccctttt
     2521 cagtcaagcg ctgtacatga tcttctcctc tgcgtttgga aatgccatag taagtttgag
     2581 gttgtgctgc cgttgactca tccaccatac cggttgttct tccgtaaacg gctgtgctgc
     2641 tgagataaat aagccggttg atttgcagat cttcagcagc atagaccacc tcagctgtac
     2701 cccaatggtt gacctgctca aagaagtgcg gatcctggtt ggcaaaaggt gtagtaacac
     2761 gtgctgccag gtgtactacc aggtctttcc cggccatggc tttccgcaat tttctggtat
     2821 ccagaatatc agctgtgatc agttcgatct tgttggcccc gactaccgga tcaaaaaaga
     2881 tggatccgtt atgacgtgag aaattatcat acaagctgac ctgcgttact tcagtgagcc
     2941 ggcacaaagc tttagccagg ttaaatccga tatatccggc tccaccggtg atcagaatct
     3001 tcattttttt tctatcagtt cagtatgtaa gccacattcg gttttctgca tgccaaacca
     3061 tcggccaccc ctttcgtggc tcaggagtgc caggtcagcc ctgcgggtac agggttcaca
     3121 gcctatgctg acataaccct gcatatcaag cggatgggta ggcaggtcaa attgggcaat
     3181 ataatcccag atcattttct gggtccagtc aatgaccgga tgataacgca cagccttgcg
     3241 atgtgttggc tgttctacag taaatgaggc ccggttatca ttctgatcgc cacgcacacc
     3301 gttaatccag acatcaaact ggtccagcag cggctccatg ggtgccactt tattcagata
     3361 acagcaatag tcagggtctg aggtgtacag caaattgcct ttttcattga gttgcatgat
     3421 cctgggtgtg gtggatgcca ccgatctgat ctgaatacca agctgagaag ctacttcatc
     3481 gcgaaagcga atggtttcag gaaaatgata ccctgtttgc aggaaatata cagggatgct
     3541 gtcatcaatc ctgctgatca tatgcagcaa gggtatgcta tgtgtctgga aggaggaaga
     3601 aaggaacaag gtctttcccg cttcctgata gctttgaatt tgttgggcta tctgttcaaa
     3661 cgatggcatc agctaatact cttcgccagc agatctatca gatgaatgca tttgacagga
     3721 atctgctgtt tatcaaaata ggcctgcagg tgcatcaggc aggaataatc ggtagaaacc
     3781 acatactcag cacctgtggc tgctgcaaat tgccctttgt tgtcggccat tccggaagaa
     3841 atcgcttcga atttggcagc gaatgttccc ccgaagccac agcatgtctc tgaaaaatcc
     3901 atctccacca gctccagccc ttccacagct gccagaagct ttctgggttg ctcttttatg
     3961 ccacattcac gcagtgctcc gcaggcatcg tgataggtca ctttggcctg gaacttccct
     4021 cccaggtctg tttgctgcat tacatcaacc aggaattcac tgatctcaaa aagatttcgc
     4081 tttacctgct tgtagtagtt atgatagggt gtattggcat acagttcatt gtagaagttt
     4141 ctgacaaaac ctgtgcaaga gccactggga gaaacgatag ttcggtcaac cgcactgaaa
     4201 tcggtaatga acttcttacc cacatcatcc attttttccc attgaccggc attgaaagcc
     4261 ggctgcccgc aacaggtctg ccctggatta tagtgtacct tacagcccac atgttccagt
     4321 accttgacca tgttaaaacc ggtatcaggc gatagctggt caatgaaaca gggaataaat
     4381 atatctacct ccatagtgat ggcgacaaat gtaatacaag gaagggataa cgctgattag
     4441 ttgcccttta ccaccaggat cttggtgtct tctatcgggt tacccctgat cacgtagtca
     4501 tcgtggttat tgctattcag ggtgaccgat atttcgtagg ttccgggagg cattgcagcc
     4561 aggtggtagt attctccata gatccgggcc actttttctc cgttgatatg caaatgtgca
     4621 tgtccttcat acatgcggtt tgacgtattg acgttttccg gagtgaattt gaaattggtg
     4681 gtcgttaccc tgatattata tccacccatc ggatcatcat tgatctccat gcgcacatca
     4741 ggcaattcat ctggcggata tccggccagt tcgatctgac catgcatatg agtggtgggc
     4801 ttattagaca aaaacttctg tttccgttca tatttgttgt tggccagaag gtggtaggca
     4861 aagagcagag agaacagcaa aaccagaaac ccggacgttg tgaacagtat gcgcttgcca
     4921 atagtatgaa acttgcggtg cagaaaatgg tacacatcgc ccgggccttc cagcaggaaa
     4981 actacgagag caccatgtac cagtgtatga cctatcactt caactttgcc aaaaactgcc
     5041 gtggtaagaa aaaaaacgat ggtgatcagc accgccagtg ggcgctgcaa taaattgact
     5101 atcaacagat agcccagact aaactcaaca aaagctgcag atgatatgaa gaagtcataa
     5161 ttaaggccca tagcaagttg cggatgctct gtgaccagaa acatagacca gtccttataa
     5221 atgaattttt ccagcgccac ccagcatagt gaaaaaccaa cagtcaggta cagaactgtg
     5281 agtccagatt gtttcagctt cacatgcctg ctggaagaag tggccagata gtagccaaca
     5341 cctgtaaaca gggcatagtc cagcatgtga aaaggatcat agatgacaca gcccagcagg
     5401 tataaaatga tggttcctat tccggcataa ggaacggtct tgtcaaaaag cagcagaaag
     5461 gcaagcagga actgaaacat gcccacccat tcgtatttgc ttggcaccat cagcgtagga
     5521 gccagcatag ctccaccctg ccaggaaagc agaaaaagca tacctgcggc aatacgcatg
     5581 accatgacag acgatgaccg tttgctttcg aaccaaccgt ccagatcacg gtaccactta
     5641 ctgctggcga tctgcctgtc cagccatacg cccactgaaa tgaccaccag tgccagcccc
     5701 aacagccaga taaacacatt tgatactacc tccgggacag acagaggctt atcggcatac
     5761 gatccgtcag taaaccattt tacgtgtgca aaagcctcat tcctgaatgt gaggagtaac
     5821 aggaacaaca gtggcaaacg catgggaaaa gagcgcaaca actgtttcaa aaccatgtgg
     5881 ttgttcagct gcgaaattaa ggtagtattt tagtgcgatg accattctgt agggcagttt
     5941 ttgtaaaaga attgttaacg caggtgcaca gtggtcatgg aaactgcaat aagatgtcag
     6001 atattcgttc caactggcgc acatgaaatc gctaaagatc aagataatct tatggtttgt
     6061 actcggtatc tcgcttgccg gagcaggtat ctgggtggtt tatgacagtt tccagcaatt
     6121 gctggcagct gttcaggttc tgaccgcacc ggataaagca tatgaagcag cagatcagat
     6181 acgtacagac ctggtgatca ctgaaaacag catgcgtgcc tatctgatgt ctggagacaa
     6241 tgaggaactg gacacctatc tggaaggtat ggacaggatc gaactgtacc tggacacgct
     6301 gcctcagatc caggtaaaag ggatggacga tgatgatatt gatgagatcg taaccctgct
     6361 tcaaacccgg gctgatctca tgtacgatct ggttgagctc aagaccgacc agcgtgaaca
     6421 aagtctggct gccaaaacac tggggcagtt tcaggatcag ctggctgcca ggaacagaca
     6481 ggcacgagac cagccatcaa cgattcagac agattccgtt ccgggcctga aagccagcaa
     6541 tgccagtcag ccggctgatg aggaaaaaga aaagaaaaga ctgctgaatc tgttctcgaa
     6601 aaagagcaga gaaaaagaca acaccactgt acaagctgat tccattgcag aatcagtacc
     6661 tgaagaaaca cagaaaacca gattatctgc cactggagta caatacagca gcgacctgga
     6721 catggaacag gtgaggaaga tcctccgtga catgggcaga gaggaagccg tcagtaatgc
     6781 tttgctggat gaaagagaac tggagatcct ggccagcgac cggcaactga tggaaaaggt
     6841 caatgccatc atggacagaa ttgaaaagcg caatgcggaa taccaggctg cagtcataaa
     6901 ccaggccaat caacaggtaa cagatacttc agttcagctt ttccgtgcgg taattggtgc
     6961 cattcttgcc ggtttgatcc tgcttaccgt actgatgatc gatatcaaca ggatgcacag
     7021 gtaccgaacg caactggaag aaagcagaga ctacgcccag cgtctggcta ccgccagaca
     7081 acggttcctg gccagtatga gccatgagat acgcacccct ctgaacgcca ttatcggctt
     7141 cagtgaacaa ctgcaacaaa acggaacctc agagaccaaa gaacaggcag atttcataca
     7201 tagcgcatca ggtcacctgc tggagatcgt caatgatatt cttgattatt ccaaactgga
     7261 gtttggaaag gccagcttgc atcaggctcc ctttacgatg caggaagtac tggatgaagt
     7321 gcacggaatc ctcaaagcca aagccgaaga aaaacaattg acctggtcac tggagcttga
     7381 taacggatgt cgcaaatggg tggaaggcga tgccttgcgt ttgaagcagg tgcttatcaa
     7441 tcttgcaggt aatgcgatca agttcacctc atccggtagt gtgaccatta aagttggtac
     7501 cgtagaaaga gaccgtttca ctgatttccg catcctggtc attgacaccg gtattggaat
     7561 agacaagaat gcgcaggctc gaattttcga cgatttttcg caggcagaca gttccacttc
     7621 cagatcgtat ggaggcacag gtctcgggct ggctatctgc aaaaagatca ttgagcaaca
     7681 tcacggtacc atcagggtag aaagtgaacc cggaagcgga accaccttta ttgtggaact
     7741 gagttaccgg aacaccaaag aggctgcaac tgaaaagaaa acttccagag ccgggcggat
     7801 ggaattgaaa ggcatgaaaa tactggtggc cgatgatgaa cttttcaata tcagactggc
     7861 caccatggtt ctggaaaaag caggagcaac cgtaacctct tgtcagcagt cagacgatat
     7921 ggaatcctgc cttcgtgtgc gttatgatgc ctatctgatc gatcttcata tgccggggcg
     7981 ggatggtgta tctgtagcac atgccattcg caccaaacac cctgatgcca gg
//
LOCUS       DAOMAX010000113         7985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1345_length_7985_cov_4.214376, whole genome shotgun sequence.
ACCESSION   DAOMAX010000113 DAOMAX010000000
VERSION     DAOMAX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_7985_cov_4.214376"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PK742_07215"
     CDS             complement(<1..1697)
                     /locus_tag="PK742_07215"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQU76417.1"
                     /translation="MVFDLQMISGVYDRYEARVNAARQLLNRPLTLTEKILYTHLSEG
                     MPSAAYERGQAYVNFDPDRVAMQDATAQMALLQFMSAGKAKVAVPSTVHCDHLIQAKT
                     GADTDLATALDTNKEVFDFLASVSKKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGFPWELKMPKLIGVKLTGKMNGWTSAKDVILK
                     VAGILTVKGGTGAIVEYFGEGAENISCTGKGTICNMGAEIGATTSVFGYDDSMRRYLR
                     ATGREEVVQLADKVAAHLNGDAEVYDNPEKYFDQVIEINLSELEPHVNGPFTPDLAWP
                     ISKLKDAVQVNGWPATLEVGLIGSCTNSSYEDLTRAASVARQAASKKLKVKSEYTITP
                     GSEQVRFTVERDGLLNDFEQIGGVVLANACGPCIGQWARHTSDPDKKNTIITSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALAIAGDITFNPITDKLMNEEGVEVMLDEPTGIELPE
                     MGFAVEDAGYVAPAEDGSGVNVVIAPDSNRLQKLEPFKAWEGTDLTGLKLLIKAKGK"
     gene            1795..3024
                     /locus_tag="PK742_07220"
     CDS             1795..3024
                     /locus_tag="PK742_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU76418.1"
                     /translation="MDLQENIKLATKAIRGNLLRTILTVCIIAFGLMALLGILTSIEA
                     LKSSINRNFATLGANSFSIRNEGQIDGGEGGGDREPSPMITRRQAEDFRNRYAYPSIV
                     SISYVPFNGWAKLKAGEKETNPNVSVMAVDENYLTVAGYEMESGRWFTGGEIESATRY
                     VILGSDVLHKLFGEEDPLGKGVSIQNQAFRVIGVLKSKGSSMLSSDNRVFIPVSTSRS
                     LFPGQENSYTITVGVNTVDDLEPAIGSATGIMRQVRRLDVREGNDFSVFKSDSIIEVV
                     LSNISYVTLGAIIIGLITLLGAAIGLMNIMLVQVNERTREIGISMALGATGQIIKRQF
                     LTEAIVICLFGGAVGILLGVLTGNGVASFFKVNFFIPWGWVLAGLALTFVTGLAAGYY
                     PALKASKLDPIEALRYE"
     gene            3182..3568
                     /locus_tag="PK742_07225"
     CDS             3182..3568
                     /locus_tag="PK742_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HQU76419.1"
                     /translation="MLTLEQLSYRSLNAAFKVHKRLGPGLLESVYETCLVYELELNGL
                     YVKRQVPLPVIYDDIKIDGGYRIDILVEDRLVLEIKSVESLNDLYTAQVLTYLKLGGY
                     RLGLLLNFNTPQLKQGIKRLIMGYDE"
     gene            complement(3621..4067)
                     /gene="rplI"
                     /locus_tag="PK742_07230"
     CDS             complement(3621..4067)
                     /gene="rplI"
                     /locus_tag="PK742_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQU76420.1"
                     /translation="MEVILLKDVENLGDANAVVKVKDGYGRNFLIPRGLAVVANDGNK
                     RMLEELKRQTSIKEAKMMAEVEKMAGQFKDNVIRVGAKVGQNDKIFGSVTNVQLAEAI
                     RTQLGVLVDRKKISMEEDVKSLGNYSAHISLSAEKKVEVKFEVVEE"
     gene            complement(4072..4338)
                     /gene="rpsR"
                     /locus_tag="PK742_07235"
     CDS             complement(4072..4338)
                     /gene="rpsR"
                     /locus_tag="PK742_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQU76421.1"
                     /translation="MAQNKNIRFLAATKLGVTRKKYCRFKKSGIRYIDYKNPDFLLKF
                     VNEQGKILPRRLTGNSLKYQRRVSQAIKRSRQLALMPYVTDLLK"
     gene            complement(4341..4709)
                     /gene="rpsF"
                     /locus_tag="PK742_07240"
     CDS             complement(4341..4709)
                     /gene="rpsF"
                     /locus_tag="PK742_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQU76422.1"
                     /translation="MNQYETVVVLTPVLSDDDVKRTLEGYKELLKNNGAEMVHEELWG
                     LKQLAYPIKKKTTGIYFVMEYKASGEAVAKMEVQFNRDEQVLRYLTIRLDKFSVDYNE
                     RKRRGEIGKNRKPAEATENA"
     gene            4874..5698
                     /locus_tag="PK742_07245"
     CDS             4874..5698
                     /locus_tag="PK742_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76423.1"
                     /translation="MRKETAFLAMLTIATLSGCRRERLQESFVAIDNGLIVNALDETQ
                     SIVNQEAGNHGFAGLSNGSGEVFRTAADCPEVTFSAPIGTFPNTMTIDFGTGCTGYYG
                     VERSGKIIATFTGPYPEAGTVITTTTEDYYVNGNLVEGTRTVTNMGTNDAGNIWFTVV
                     TEGGHVHLSSGEDIYANINHEREWIEGAGGLPVWDDVYLISGTESGTNRFGIDFTATI
                     TDPLRRELDCRWPTSGTRVISTADHPDREIDFGDGECDNIATVTVDGDSFEIELPL"
     gene            6140..7294
                     /locus_tag="PK742_07250"
     CDS             6140..7294
                     /locus_tag="PK742_07250"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQU76424.1"
                     /translation="MQQVKGHDLTLHILGGGQLGKMLIEAASDWNIRCHVLDPDPDCS
                     CSRLAWQFTCGSFKDYDAVMAFCADAHYITIEIEHVNTDALRDLKAQGKQVFPDPDML
                     DIIKDKGKQKSFYAEHGIPTSPFTLYDDADAIRQAVASGTRTLPFVQKSCTAGYDGKG
                     VQVVRTKDDLDKLLPSASLAEELIPFEKELAVIVARSTNGDTIAFPAVEMEFHPTANL
                     VEFLLSPAHINSELEQKAQQLAVRTIEAMNLYGVLAVEMFLTADGDILVNEVAPRPHN
                     SGHHTIEANITSQYQQLLRSIFGLPLGSTALSTTSMMVNLLGEDGYTGEAIYEGLPEC
                     LAMEGVYVHLYGKKITKPFRKMGHVTIVDSDAAKAREKANFVKDNLKIKA"
     gene            7291..7791
                     /gene="purE"
                     /locus_tag="PK742_07255"
     CDS             7291..7791
                     /gene="purE"
                     /locus_tag="PK742_07255"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQU76425.1"
                     /translation="MKPVVSIIMGSDSDLSVMKDAAEVLEHFGIPVEVTVVSAHRTPQ
                     RMVDFATHAHERGIRVIIAGAGGAAHLPGMVASITPLPVIGVPVRSSNSIDGWDSILS
                     ILQMPNGVPVATVALNAAKNAGILATQILANSDADLQQKVLAYKQDMKETVEKKAKKL
                     EEKGWK"
BASE COUNT         1913 a         2121 c         1954 g         1997 t
ORIGIN      
        1 cacttgcctt tggctttgat gagcagtttc agtcctgtca ggtcggtgcc ttcccaggct
       61 ttgaatggtt cgagtttctg caggcggttg ctgtcaggag cgatgaccac attcacaccg
      121 gaaccatctt cggccggagc tacatagccg gcgtcttcca ctgcaaatcc catttccggt
      181 aattcgatac ctgtgggctc atccagcatg acctccacac cttcttcgtt catcagttta
      241 tcggtgatgg ggttgaaggt gatatcaccg gcaatggcaa gagcggtcac gatctccgga
      301 ctggccacga atgcgtgggt gttcggatta ccgtcattgc gcttggcaaa gtttcggttg
      361 aaggaagtga tgatggtatt cttcttatcg ggatcgctgg tatgacgtgc ccactgaccg
      421 atacagggtc cgcaggcatt ggcaagcacc acaccaccta tctgttcaaa atcgttcagc
      481 agcccatcgc gttcaacggt aaaacgcacc tgctcagaac ccggtgtgat ggtgtattcg
      541 ctcttcactt tcagtttctt gctggcagcc tgacgggcca cagaggcggc acgggtcagg
      601 tcttcatagg aagagttggt gcaggaaccg atcagtccga cctccagggt agcaggccag
      661 ccgttcacct gcacggcatc tttcagtttg ctgatgggcc aggccagatc cggagtgaac
      721 ggaccattca catgcggttc cagttcgctc aggttgatct cgatcacctg gtcgaaatat
      781 ttttccgggt tgtcatacac ttcggcatca ccatttaggt gagcagcaac cttatctgcc
      841 agttgcacca cttcttcgcg gccggtggca cggaggtatc ggcgcatgct gtcatcgtat
      901 ccaaaaacag aagtggtggc accgatctca gcacccatat tacagatggt gccctttccg
      961 gtgcagctga tgttttcagc accttcacca aaatattcca cgatggcacc tgttccgcct
     1021 tttacggtca ggatacctgc cactttcaga ataacatctt tagcgctggt ccagccgttc
     1081 atcttaccgg tcagcttcac accgatcagt tttggcatct tcagttccca ggggaatccg
     1141 gccatcacat ccacagcatc cgcaccaccc acaccgatag ctaccattcc cagaccaccg
     1201 gcattcacgg tatggctgtc ggtaccgatc atcataccgc ccggaaaagc atagttctcc
     1261 agtactacct ggtggatgat acccgcaccg ggtttccaga aaccgatgcc atatttctta
     1321 ctcacagaag cgagaaaatc aaaaacttcc ttattggtat ccagcgctgt ggccaggtcc
     1381 gtatcagctc ctgtcttagc ctggatcagg tgatcacaat gcaccgtact gggaactgct
     1441 actttggctt ttcctgcact catgaactgc agcagcgcca tttgtgcggt agcatcctgc
     1501 atggccacgc ggtccggatc gaaattgaca taggcctgtc cgcgttcata ggctgctgaa
     1561 ggcatgcctt ctgagaggtg ggtgtacagg atcttttcgg tcagggtgag ggggcggttg
     1621 aggagctgac gggcagcgtt cacacgagct tcgtagcggt catatacgcc gctgatcatc
     1681 tgtaggtcaa ataccatgct gatattgggg ttatttttaa caaaagcgac tgcaaagtta
     1741 acaataagag ggcgggttaa gtttgattta ccgttatttt tacagcaatt agcaatggat
     1801 cttcaggaaa atatcaagct ggccaccaaa gccatccgtg gcaatttact acgcactatt
     1861 ctgaccgtct gcatcattgc attcggtctg atggcgctgc tgggcatcct gacctccatt
     1921 gaggcgctga agtccagcat caaccggaat tttgccaccc tgggtgccaa cagtttcagc
     1981 atccgcaatg aaggtcagat agatggggga gaaggtggcg gcgatcgtga gccgagtccg
     2041 atgatcaccc gcaggcaggc ggaagatttt cgcaatcgct atgcatatcc ttccatagtc
     2101 agtatcagct atgtgccttt taatggatgg gcgaaactga aagcaggcga gaaagagacg
     2161 aacccgaatg tctcggtgat ggcggtggat gagaattacc tgacggtagc gggctatgaa
     2221 atggagtccg gccgatggtt caccggtggc gaaatagaat ctgccacccg ctatgtgata
     2281 ctcggttctg atgtgttgca taaattattc ggggaagaag acccgctggg gaaaggtgtg
     2341 agcatacaga atcaggcctt ccgtgtcatc ggtgtgctga agagtaaggg cagcagcatg
     2401 ctgtcgtccg ataaccgtgt ctttattcct gtgtccacct cacgttcctt gtttcccgga
     2461 caggagaaca gctacaccat aaccgtaggt gtgaatacgg tcgatgacct ggaaccggca
     2521 attggttctg caaccggcat catgcggcag gtgcgcaggc tggacgtgcg ggaaggaaat
     2581 gatttttctg tgttcaagag cgacagcatc atagaagtgg tgttgtctaa catttcctat
     2641 gtgaccctcg gtgccatcat catcgggctc atcaccttgc tgggggctgc catcggattg
     2701 atgaacatca tgctggtgca ggtgaatgag cgcacccgtg agataggcat cagcatggca
     2761 ctcggtgcca ccggacagat catcaaacga cagttcctca cagaagccat tgtcatctgc
     2821 ctctttggtg gtgctgtggg catcctgctg ggtgtgctga ccggcaacgg cgtggccagt
     2881 ttcttcaaag tgaatttctt cataccctgg ggatgggtgc tggccggtct tgccctgacc
     2941 ttcgtgaccg gcctcgctgc tggttactat cccgcactca aagcctccaa actggatcct
     3001 atcgaggcgc tgagatacga gtaaacgggt ttcaaatggc ttaacacaaa ggcacaaagg
     3061 aggcacagag aactcaatgt atggcctttg cgatctctgt gtttgtaact gcttgcagtt
     3121 accagtgtca tcgtgttaat agtttatata acattattaa aataatatca tatgtttgag
     3181 catgcttacc ctcgaacaat tatcttatag atcattgaat gctgcgttta aagtacacaa
     3241 gagattaggg ccgggtttat tggaatcagt ttatgagacc tgcctggtgt acgaacttga
     3301 attgaatgga ctttatgtca agcgtcaggt acccctaccg gtgatttacg atgatatcaa
     3361 aattgatggt ggatacagaa ttgatattct agtagaggac aggcttgtcc tggagataaa
     3421 atctgttgaa agcttgaatg atctttatac tgcgcaggtt ctgacttatt taaagttggg
     3481 tggttatcgg cttggattat tattgaattt caatacacca caactaaagc aagggatcaa
     3541 gcgacttatc atgggctatg atgaataaaa aacggctgcc ctttggaggc agccgttccg
     3601 ctataaaagc ttcttcttcc ttattcttcc acaacctcaa atttcacttc taccttcttc
     3661 tcggcagaga gggaaatatg ggcactgtaa ttacccagtg atttaacatc ttcttccatg
     3721 ctgatcttct tacgatcaac cagcacaccc agttgggtac ggatcgcttc ggcaagctgc
     3781 acattcgtta cgctgccaaa gatcttgtcg ttctgaccca ctttggcacc tacacgaatg
     3841 acattgtctt tgaactgtcc ggccattttt tctacctctg ccatcatttt ggcttccttg
     3901 atagaagtct ggcgcttgag ttcttccagc atgcgcttat taccatcatt ggcaacaact
     3961 gccagcccgc gtgggatcag gaaattacgg ccgtaaccgt ctttcacctt caccacagcg
     4021 ttcgcatcgc ccaggttttc tacgtctttt aacagaatga cttccatgat cttactttaa
     4081 caggtcagtt acataaggca tcagtgccag ctggcgactc cgcttgatgg cctgagacac
     4141 acggcgctga tatttcaacg agttaccggt gaggcggcgg gggaggatct tgccttgttc
     4201 gttcacgaac ttcagcagga aatccgggtt cttgtagtcg atgtagcgga tgccactttt
     4261 cttgaaacgg cagtacttct tgcgggttac acccagtttg gtggctgcca ggaaacggat
     4321 attcttattc tgtgccatga ttatgcgttt tcagtggcct ctgccggttt gcgattcttg
     4381 ccgatctcgc cgcggcgctt gcgctcgtta taatcaacgg agaacttgtc cagacggatg
     4441 gtcaggtaac gaagcacctg ctcatcacga ttgaactgaa cttccatctt tgccactgct
     4501 tcaccggagg ccttatactc cattacgaag tagataccgg tggtcttttt cttgattggg
     4561 taggccagtt gcttcagacc ccagagctct tcgtgtacca tttcggcacc gttgttcttc
     4621 agcagctcct tgtagccttc cagggtgcgc ttgacatcgt catcgctgag caccggagta
     4681 agcaccacca ctgtttcgta ttggttcatt gctatcagtt atcaatataa aatccggact
     4741 tcggcccgga cgggctgcaa agttacaaaa caggccagaa ataatccaaa tagcaggcga
     4801 cctgaatttt ttggactgcc cggcttaaac ttccggcttt ccacaccgtc caaacaggca
     4861 aaaaacaacc cctatgcgca aagaaacagc ttttctggcc atgctgacca tagccaccct
     4921 cagcggttgc cggcgggaac gtctgcagga atccttcgtg gccatagaca acgggctcat
     4981 tgtcaatgcc ctggatgaga cccagagcat cgtcaaccag gaagcaggaa accacggttt
     5041 cgcagggctg agcaatggtt cgggagaggt attccgtacc gctgccgatt gtccggaagt
     5101 gacattctct gccccgatag gaaccttccc caacaccatg accatagatt tcggcactgg
     5161 ctgcaccgga tattatggtg tagaacgctc gggcaagatc atcgccacct ttacaggtcc
     5221 ttatcccgaa gcaggtacgg tcatcaccac tactacagaa gattactacg tgaacggcaa
     5281 cctggtggaa ggtacccgca ccgttaccaa tatgggcacc aatgatgccg gaaatatctg
     5341 gttcaccgtg gtaacggaag gcggacatgt acacctgtct tcaggagaag atatctatgc
     5401 caacatcaac catgaacggg aatggataga aggtgccgga ggattgcccg tgtgggatga
     5461 tgtctatctc atctccggaa cggaatccgg caccaatcgc tttggcatcg atttcactgc
     5521 caccatcacc gatccgctgc gcagagaact ggactgccga tggccaacat ccggcacccg
     5581 tgtcatctcc accgccgatc accccgaccg cgagatcgac ttcggcgatg gcgagtgtga
     5641 taatatcgcc accgtcaccg ttgacggcga cagtttcgaa attgaattac cgttgtaata
     5701 gtggtgttgt catcaatgaa cggctccaat ttttggggcc gttcctgcat atgagatgct
     5761 tgaaagtgag aggtgagaag tgaatggtga atggttttgc cagatcaatt caggatcctc
     5821 gtaatataaa tgcaccacac accccagacc actcaccgct catacaaccg gtacgggact
     5881 cattctctcg ttccctcatt ctcccattag ctcatcccct cattatctaa ttctctaatt
     5941 tccacatttc cccattcccc cattctctaa tttccacatt ttcccatccc ctcatcggct
     6001 cattccctca ttatctaatt ctctaatttc cccattttcc aattccccca ttttctgatt
     6061 ctctcatttt cacattctcc cattccctcc tttccacatt ctcccattct cccatcccct
     6121 cattggttac ttttgcccca tgcaacaagt caaaggccac gacctgacct tacacatcct
     6181 tggtggcgga caactgggca agatgctgat agaggctgcg tccgattgga acatccgttg
     6241 ccatgtgctg gatcctgatc ccgattgttc ctgcagccga ttggcatggc agttcacctg
     6301 tggcagtttc aaggactatg atgccgtcat ggccttttgt gcagatgcac actatatcac
     6361 catcgagatc gaacacgtca acaccgatgc cctccgcgat ctgaaggcgc agggcaagca
     6421 ggtctttccc gacccagaca tgctggacat tatcaaggac aaaggcaaac agaaaagctt
     6481 ctatgcggag catggtattc ccaccagtcc gttcacctta tatgatgatg cagatgccat
     6541 cagacaggca gttgcttcag gtaccagaac gctgccgttc gtccagaaaa gttgtacagc
     6601 cggatacgat ggcaaaggcg ttcaggtagt gcgtaccaaa gacgatctgg ataaactttt
     6661 accaagtgca tcactggcag aggaactcat cccctttgaa aaagaactgg ccgtgatcgt
     6721 ggcccgcagc accaacggcg ataccatagc gtttccggca gtggagatgg aatttcatcc
     6781 cacggccaac cttgtcgagt tcctgctgag tcctgcgcat atcaacagcg aactcgaaca
     6841 gaaagcacaa caactggctg tacgcaccat tgaagccatg aacctgtatg gagttttggc
     6901 agtagagatg ttcctcacag ctgatggcga tatcctggtg aacgaagtag ctcctcgtcc
     6961 ccacaacagc ggccaccata ccatagaagc gaacatcact tcccaatacc agcagctgtt
     7021 gcgcagcatc ttcggattac ccctcggaag tacagccctc tccaccacca gcatgatggt
     7081 caatctgctg ggtgaagacg gctacaccgg cgaagccatc tatgaaggtt tacctgaatg
     7141 cctcgccatg gagggggtct atgtgcacct gtacggaaag aagatcacca agcctttccg
     7201 caagatgggt catgtgacca ttgtggacag tgatgcggca aaggcaagag aaaaggcgaa
     7261 ctttgtaaag gacaacttaa agatcaaagc atgaaaccag tggtcagcat catcatgggc
     7321 tccgattccg atctctccgt catgaaagac gccgcagagg tgctggagca tttcggcatt
     7381 ccggtggagg tgaccgtggt atcagctcat cgtactccgc agcgtatggt cgatttcgcc
     7441 acccatgccc atgaaagagg tatccgtgtc atcattgccg gcgccggcgg agcagctcac
     7501 ctgccgggca tggtagccag catcacgcca ttacctgtta tcggcgtccc tgtaagatcc
     7561 agcaactcca tcgacggctg ggacagcatt ctttctatac ttcaaatgcc caacggcgta
     7621 ccggtagcca cggtagccct caacgctgcc aagaacgcgg gcatcctcgc cacccagatc
     7681 ctcgccaatt cagatgccga tctgcagcag aaggttctcg cctacaagca ggacatgaaa
     7741 gagacggtcg aaaagaaagc taagaagctc gaagagaaag ggtggaaata aaaggagcaa
     7801 gcaacctcct gccagccagt tacggagacc ggctttccgt tgtagtctcc ggctcagata
     7861 ttcaattata ggcctacata atcccgtttt gccggagagc tgccactgca atccgggcta
     7921 ggaaatatat tggtgccttc gttgccaccc tgaatgaatc agatacctga tatacatctg
     7981 ttaaa
//
LOCUS       DAOMAX010000114         7885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1373_length_7885_cov_4.132439, whole genome shotgun sequence.
ACCESSION   DAOMAX010000114 DAOMAX010000000
VERSION     DAOMAX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1373_length_7885_cov_4.132439"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PK742_07260"
     CDS             <1..1009
                     /locus_tag="PK742_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76426.1"
                     /translation="QVKSTISYQASDKVRVKGGAENWTIDNYQLAGSQRFESGYNLTG
                     SFVEADIFITNDLAGRVGVRHEYTDILQEHNVAPRLSLAYKIGENSQLGAAYGDFYQV
                     PQRQFLYVPDQVTSLIYEKATHYILNFQTIKDDRTFRVEVYQKDYDHLISFTPGAFQG
                     VYEELSNDGYGYARGVDLFYRDRKSIKNADFWISYSFLDTKRRFLDYPIETQPTFAAD
                     NVLSVVYKQYVSKIRTQFGATYRYISGWPVYDPWSDTFLGETSPVYQDLSINASYLTR
                     IADQFTVVFASVNNVLGLNQVFGRTYSTEDPGVYVDQKPPLVTSIFLGMFINISYTQA
                     P"
     gene            1040..1702
                     /locus_tag="PK742_07265"
     CDS             1040..1702
                     /locus_tag="PK742_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76427.1"
                     /translation="MKQSILLFLLIGSATLYAQSPKFHDAMSQAITELEKDTTVEQLM
                     AVSNRFERIALAEKDQWLPYYYAAYARSIAGFITTDKSKVDEVLDGAQKFADIADSLS
                     PGNSEVVLLKGMVLAGRISVDPMSRGQQFGMQSGMLMNQAMQLNPENPRAYLMIGQGL
                     FYTPEQFGGGKDKGCAMLEQSRMKFETFEPESDIHPNWGENLLGQMMAPCGGGSSVPH
                     QE"
     gene            1702..2097
                     /locus_tag="PK742_07270"
     CDS             1702..2097
                     /locus_tag="PK742_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQU76428.1"
                     /translation="MAADITSREDIQLLVESFYKRVREDEMLAPIFRDLTHFRWDTHI
                     PVMVSFWETVLLGASSYAGNPMQKHIALHRDHPLSEKHFDRWRNLFFATLDELFSGPV
                     TNDAKQRVFSMETLMLFKLNASDKEGFIQ"
     gene            2094..2549
                     /locus_tag="PK742_07275"
     CDS             2094..2549
                     /locus_tag="PK742_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HQU76429.1"
                     /translation="MKKGVVSIWLLVLSILVRAQTFTGPEEDIDALLDNMKAFSAAYV
                     GADYDLIMNSYTTDASIFPPGAEIISGREAIRERWILPEGRRVLEHNIEPVSITVDGD
                     TAYDHGHYSGISQNGDQDPVSWKGKYVIVWKKVDGNWLMYLDIWNGINE"
     gene            2551..3000
                     /locus_tag="PK742_07280"
     CDS             2551..3000
                     /locus_tag="PK742_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HQU76430.1"
                     /translation="MKSLFEQEAAAELQHRLSELEQDARPGWGKMDVAQMLAHCSAGL
                     DMAAGRIHPKGTFLGKLIGRRMRPLYSSDKPMGKNSPTARELIMVEDRKFLDEKQHLA
                     ELINSFHDGGPAGCTTSPHPFFGPLTPEEWGKGMYKHIDHHFRQFGI"
     gene            3002..4144
                     /locus_tag="PK742_07285"
     CDS             3002..4144
                     /locus_tag="PK742_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQU76431.1"
                     /translation="MDSQKHLFQLPADSVYLNAAYMSPLLKSVEEAGIVSMQQKRNPV
                     TIGRDDFFTGTDALKTSFGKLIQGDAAQCVIIPSSSYGFATVFQNLKSNGRKKALTIA
                     DEFPSGYLTLAHWCNTHKVELTIVDPGPGAASRDWNERLLDAIDQNTSVLLMSAVHWM
                     HGIQYDVEAISARCRETGCRLVVDGTQSVGALPFDLGKTPVDALVCAGYKWLLGPYGI
                     GMMWLDPSFNDGLPLEFNWINRSNSRDFARLTNYSLDYTPGAGRYNMGETSSFIHVAM
                     LQAALEQIHTWTPVAIQSHTLEIRNHLRLLLSETRPALLTDKGYSGHLFGLTLPIGTG
                     AEQLAARLAKERISVSVRGNFIRVSPHVYNDQQDAEKLSAVLIRYL"
     gene            4234..4761
                     /locus_tag="PK742_07290"
     CDS             4234..4761
                     /locus_tag="PK742_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76432.1"
                     /translation="MKKVSLIFLAWITLLFVSGCAGFNRVEFDHSIQVSFNINPVNPG
                     SYTYAEVLYTNDLEQVLNDHKASLDHLESLKLKGFEIWITAPDGATADPFDNLFVYVE
                     AEGHNEQLLATLDPVPDGGQQYIAFKAETDVELAGYFRESEITFRLKGANSGTTTEVT
                     TFDATVYFKLEASVL"
     gene            4789..5121
                     /locus_tag="PK742_07295"
     CDS             4789..5121
                     /locus_tag="PK742_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQU76433.1"
                     /translation="MPFIDFQTGHEHTIWDGIHGRMHHSEQLTFGHFRIDAGAILPAH
                     SHPHEQWTHVVEGELEFTIDGETTVLKPGMAAFIPSNAEHSARALTACRVIDCFDPVR
                     EDFRALDQ"
     gene            5221..6024
                     /locus_tag="PK742_07300"
     CDS             5221..6024
                     /locus_tag="PK742_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQU76434.1"
                     /translation="MDWIEQAKEMLISYAPLVAGAILTLVIGFILAGIISRMARKAME
                     KRDFDASLTPFITSLINITIKILVLLSAASMFGFEVTSFVAILGALAFAIGMALQGNL
                     SHMAAGIMILFFRPFRVGDFIVTQGYSGTVKEIQIFNTILTTLDNRVIIIPNGAITSG
                     PLENLTANPERKVPMTFGISYRGDIDNARSVIQQVADGCEHILHDQPVDIFIESLGSS
                     SVNFTVRPWCKSEHYWDVFFYMHEQVKKAFDANSISIPFPQMDVHVQQN"
     gene            complement(6025..7053)
                     /locus_tag="PK742_07305"
     CDS             complement(6025..7053)
                     /locus_tag="PK742_07305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQU76435.1"
                     /translation="MDVGDSNELMTIANCFLQVSGQSCREVLRIEEGLINHSYRVTDD
                     AGEFFLMQRVNTTVFSHPQVIQQHMQTVCDALKDHQYPYQTLRSVQNSEGNTIYTDAA
                     GNCWRMMNWIPEALTYQSVHDASAARNAATVLAHFHKATAKIDPDALSETIPGFTALR
                     RRRDTLEACATQIADARAEDLLGEIRKLYPVMDTFLAVYEAAPKQVIHGDPKISNFLF
                     DKTGNPLALIDWDTIMPGPVWYDLGDMVRSFTNAGREDDAVHNDLFNTRIFDAIHTGY
                     SSVLPALDKNILLRAGMVVTLVQGMRFLTDHLQGNIYYRVDHPVHNLIRAENQVMLAR
                     AMSMRTGD"
     gene            complement(7044..>7885)
                     /locus_tag="PK742_07310"
     CDS             complement(7044..>7885)
                     /locus_tag="PK742_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQU76436.1"
                     /translation="LVLAGGLSSRYGSSKQEEAVGPAGELLLEYNLFDALQAGIRRVI
                     IVVRDQVPVLLTERLSFFKNRMEILYVSQSACYPSDRNRPWGTGHAVLSAAALVSGSF
                     LVVNADDLYGADMYGKAVQHANQVTNEQGFLLAYRLRDTLSENGEVSRGLCTVDEEGN
                     LLKIAEYKGIARKNGQIMSGTRILNGSALVSMNAWLLPASVLTGFDVSFQTFSKQHGA
                     DPDVEFFLPDAVSVEMNKGLFVQVRKCDGPWCGLTHPADLMQVRETILQAVLNGKYPT
                     KWM"
BASE COUNT         2099 a         1938 c         1890 g         1958 t
ORIGIN      
        1 tcaggtgaag tccaccatca gctaccaggc ctctgataaa gtgcgtgtca aaggcggtgc
       61 agagaactgg accatagaca attaccagct ggccggctcc cagcgatttg aatcaggcta
      121 caacctgacc gggagctttg tggaagctga tatcttcatc accaacgatc ttgctggtcg
      181 cgtgggtgtg cggcatgaat acaccgatat tctgcaggag cacaatgtgg caccgcgttt
      241 aagcctcgct tacaagatcg gcgagaacag ccagcttggt gctgcctacg gcgatttcta
      301 ccaggtacct cagcgacaat tcctctatgt tccggatcag gtaacctcac tcatctatga
      361 aaaggccacc cattatatcc tgaacttcca gaccatcaaa gacgaccgca ccttccgggt
      421 agaggtctat cagaaagatt atgaccacct catctctttc acacccggcg cctttcaggg
      481 agtgtatgaa gaattgtcga acgatggcta tgggtatgct cgtggtgtgg acctctttta
      541 ccgggacaga aagagcatca agaatgccga tttctggatc tcctattcct tcctggatac
      601 gaaaagaaga ttcctggatt atcccattga aacacagccc acttttgcgg cagataatgt
      661 gcttagcgta gtgtacaagc agtatgtcag caagatcagg acccagttcg gtgctacata
      721 tcgctacatc agcggatggc cggtgtatga cccctggtca gatacctttc tgggcgaaac
      781 atcccctgtg tatcaggatc tcagcatcaa cgccagttac ctgacccgca tagccgatca
      841 gttcacagtt gtgtttgctt cggtcaacaa cgtactcggc ctgaaccagg tttttggcag
      901 aacctattcc acagaagacc ccggtgtgta tgtggatcag aaaccaccac tggtgaccag
      961 catttttctg ggcatgttca tcaacatctc ttacacacag gcaccataat tgcagtatca
     1021 acccgtcaaa tcaaaccata tgaaacagag tatcctctta tttctgctga tcggttcagc
     1081 aaccctgtat gcacaaagcc ctaagttcca tgacgccatg agtcaggcca tcaccgaact
     1141 ggaaaaagac accaccgtag agcagctgat ggctgtatcc aaccgcttcg agcgcatcgc
     1201 cctggcagaa aaggatcagt ggcttccgta ttactatgct gcctatgcca gaagcattgc
     1261 aggtttcatc accacagaca aatcaaaggt ggatgaagtg ctggatggcg cacaaaaatt
     1321 cgcagatata gcagacagtc tctcacccgg caacagcgag gtggtattgc tgaagggcat
     1381 ggtgctggct ggccgcattt ctgtcgatcc tatgagtcgc gggcaacaat tcggcatgca
     1441 gagcggcatg ctgatgaacc aggcaatgca gctcaacccc gaaaacccca gggcttacct
     1501 gatgataggt cagggtttat tctacacacc tgagcaattc ggcggtggca aagacaaggg
     1561 ctgtgccatg ctggaacaat ccaggatgaa gtttgaaacg tttgaaccgg aatcagatat
     1621 tcatcccaac tggggagaga atctgctggg acaaatgatg gcaccatgtg gcggcggatc
     1681 cagtgttccg catcaggaat gatggctgct gatatcacat cgcgggaaga cattcagctg
     1741 ttggtggaga gcttctacaa acgggtcagg gaagatgaaa tgctggcacc gatattccgg
     1801 gacctcaccc atttccggtg ggacacccac attcctgtca tggtctcttt ctgggaaacc
     1861 gttttattag gtgcaagcag ttatgcaggc aatccgatgc aaaagcatat tgcattacac
     1921 cgtgatcatc cgctcagcga aaaacatttt gaccgctggc gaaacttgtt ctttgccacg
     1981 ctggatgaac ttttttccgg cccggtaaca aacgatgcca aacagcgggt attcagcatg
     2041 gaaacattaa tgctgttcaa attaaatgcc tcagataaag aaggtttcat tcaatgaaaa
     2101 aaggggtagt tagtatttgg ttgttggttc taagcatact tgtaagggcg cagaccttca
     2161 caggacctga agaagacata gatgcattgc ttgataacat gaaagcattt tcagcagcat
     2221 atgtaggggc agattatgat ctgatcatga acagttacac tacagatgcc agcatctttc
     2281 cacccggtgc agaaatcatc tctggcagag aggccatccg tgagcgctgg atcctgcccg
     2341 aaggtcgcag ggtactggaa cataacatag aaccggtttc catcacggtg gatggtgata
     2401 ctgcatatga tcatggtcat tacagtggca tttcgcaaaa cggcgaccag gatcctgtca
     2461 gctggaaagg caaatatgtg atcgtctgga aaaaagtgga cggaaactgg ctgatgtatc
     2521 tggacatctg gaatggaata aacgaatgat atgaaaagtc tttttgaaca ggaagcagcg
     2581 gctgaactgc aacacagatt gagcgaactg gaacaggatg cccgtcccgg ttggggaaag
     2641 atggatgtgg cacagatgct ggcacattgc agtgcaggtc tggatatggc tgcgggaagg
     2701 atacacccga agggcacctt tctgggaaaa ctgatcggca ggaggatgcg gcctctgtat
     2761 tccagcgata aaccaatggg aaagaatagt cctacagcac gtgaattgat catggttgaa
     2821 gacaggaaat tcctggacga aaaacagcat ctggcagaac tgatcaacag ctttcacgat
     2881 ggaggtcctg caggttgcac cacttcacct caccccttct ttggtccgct aacacccgaa
     2941 gaatggggaa agggaatgta taagcacatc gatcatcatt tcagacaatt cggtatctga
     3001 gatggacagc caaaagcatt tgtttcagct tcctgccgac agtgtgtacc tgaatgcagc
     3061 gtatatgtcg ccgttgctga aaagtgtgga agaggcaggt attgttagta tgcagcaaaa
     3121 acgcaatcct gtaaccatcg gccgggatga cttctttaca ggtacggatg cgttgaaaac
     3181 atccttcggc aaactgatac agggagatgc cgcacagtgc gtgatcatcc cctcctcatc
     3241 ctatggattt gcgaccgtct ttcagaacct gaaatcgaat ggcagaaaaa aagctctgac
     3301 catcgccgat gaattcccca gcggctacct gacccttgca cattggtgca acacacacaa
     3361 ggtggaactg accatagtgg atcccggacc tggtgctgcc tcccgcgact ggaatgaacg
     3421 actgctcgat gccattgatc agaacacttc tgtactgctg atgagtgcgg tacactggat
     3481 gcacggcata cagtatgatg tggaagcgat cagcgcacga tgcagagaaa caggatgcag
     3541 gcttgtcgta gatggcaccc agagtgtggg agccctgcct ttcgatctgg gaaaaactcc
     3601 ggtggatgcc cttgtctgtg ccggatataa atggctgtta ggaccctacg gaatcgggat
     3661 gatgtggctg gatccgtcct tcaatgacgg cctgccttta gaattcaact ggatcaacag
     3721 aagcaactcc agagattttg cccggttaac caattactct ctggattaca cacccggagc
     3781 cgggcgatac aatatgggag agaccagcag tttcattcat gtggccatgc tgcaggctgc
     3841 tctggaacag atacatacgt ggacacctgt agctatacag tcacatacgc tggagatcag
     3901 gaatcatctg cggttactgt tgtcagagac acgtcctgca ttgctgacag acaaagggta
     3961 ctccggtcac ctgtttgggc tcacactacc catcggtaca ggtgctgaac aactggccgc
     4021 caggcttgct aaggaacgca tttcagtttc agttcgcggt aattttatcc gggtttcgcc
     4081 acatgtgtat aacgatcagc aggatgccga aaaactttca gctgtgttga tcagatacct
     4141 gtaggccaat tctgatctat ctcatctttt ttattgacat ttcagatttt gaaccggata
     4201 ggtttacgat actttgcata caaatgtact gttatgaaaa aggtatcact aatttttctg
     4261 gcttggataa ccttactgtt tgtaagcgga tgcgccggct tcaacagggt agaatttgac
     4321 cacagcattc aggtgagttt caacatcaac ccggtaaatc ctggcagcta tacctatgcc
     4381 gaagtgctgt acaccaatga cctggagcaa gtgctgaatg accataaagc ttctttggat
     4441 cacctggaaa gtctgaaact gaagggattt gagatctgga tcactgcacc tgacggagct
     4501 actgcagacc cgtttgacaa cctttttgtg tatgtagaag cagaaggaca taatgagcag
     4561 cttcttgcta ctttggatcc ggttcctgat ggtgggcagc agtatatcgc attcaaggct
     4621 gaaaccgatg tagagctggc cggatatttc cgtgaatcag agatcacttt caggctcaaa
     4681 ggagccaaca gcggcacaac aaccgaggtc actacattcg atgcaacggt atatttcaaa
     4741 ctggaagcaa gtgtattgta gaataaaaaa cgtttggcat ctttgagcat gccattcatt
     4801 gacttccaaa ccgggcatga gcacaccatc tgggatggta ttcacggacg tatgcatcac
     4861 agtgaacaat taacgttcgg gcatttcagg atcgatgcag gtgcaattct gccagcacat
     4921 tcccacccgc acgaacagtg gacacatgtg gttgaaggcg aacttgaatt cactattgac
     4981 ggagaaacaa cagtacttaa accgggcatg gcagcattca ttccgtccaa tgccgaacat
     5041 tcagccaggg cacttacagc atgtcgagta atagactgtt ttgatcctgt gcgggaagac
     5101 tttcgggcat tggatcagta gaggctcctg tttacatatt tttaattaat aattattttc
     5161 tgatctgccg ataataaggt agatttggaa gtactcattc attaacaaaa taataatatc
     5221 atggattgga tagaacaggc aaaggaaatg ctcatcagct atgctccact ggtagccgga
     5281 gcgatactga cactcgttat cggtttcata ctggcgggca tcatctccag gatggcaaga
     5341 aaggccatgg agaaaagaga ttttgacgcc agcctcaccc ctttcatcac ttcgctgatc
     5401 aacatcacca tcaagatcct ggtcctgtta agtgctgcct ccatgttcgg ctttgaggtc
     5461 acctctttcg tagccattct gggtgccctt gcatttgcca tagggatggc ccttcagggt
     5521 aacctgagcc atatggcagc aggcatcatg atattgttct ttcgcccgtt ccgggtgggc
     5581 gatttcattg tgacccaggg ttactccggt accgtgaagg agatccagat cttcaacacc
     5641 atcctgacca cactggacaa ccgggtcatc atcattccca acggagccat caccagcgga
     5701 ccattggaga atctgacagc caatccggaa cgtaaggtgc ctatgacctt tggcatcagc
     5761 tatcggggag atatagacaa tgcacgttca gtgatacaac aggtagcaga tggttgtgag
     5821 catattctgc atgaccagcc ggtagatata ttcatcgaaa gccttggaag cagttccgtg
     5881 aattttaccg taaggccctg gtgcaagtca gaacactact gggatgtgtt cttctatatg
     5941 catgagcagg tcaaaaaagc ctttgatgcg aacagtatca gcataccttt cccgcagatg
     6001 gatgtgcatg tgcagcagaa ttgatcagtc acccgttctc atagacatag ctctggccag
     6061 catcacctga ttttcagctc tgatcagatt atggacagga tgatccacac ggtaatatat
     6121 gttgccctgc aggtgatcgg tcaggaatcg cataccctgc accagcgtta cgaccattcc
     6181 cgcccggagc aaaatgtttt tgtctaatgc cggtaataca ctgctgtagc ctgtatggat
     6241 agcatcgaaa attctggtat taaaaagatc attgtgcacc gcatcatctt cccgaccggc
     6301 attggtaaag gaccggacca tatcacccag gtcataccag acaggtccgg gcatgatggt
     6361 atcccagtcg atcagggcca gtgggttacc tgttttgtca aacagaaagt tgctgatctt
     6421 cgggtcgcca tgtatgacct gctttggtgc agcttcatag acagccagaa aagtatccat
     6481 gaccggatac agctttcgga tctctcccag cagatcctca gctcgagcat cagctatttg
     6541 cgtcgcacat gcttcaaggg tatccctgcg ccttcgaagt gcagtaaatc ctgggattgt
     6601 ttcggataat gcgtcagggt caattttagc tgttgcttta tgaaaatgcg ccagaacagt
     6661 tgcggcattc cgtgctgccg atgcatcatg cacactttga tacgtaagcg cctcgggtat
     6721 ccagttcatc atgcgccagc agttgccggc agcatcagta taaatggtat tcccttcaga
     6781 attctgtact gatctcagtg tctgataggg atattggtgg tccttcaatg catcacaaac
     6841 tgtttgcatg tgttgctgga tcacttgagg atggctgaat acggtcgtat tgacccgttg
     6901 cattaaaaag aattcgcccg catcatctgt gacgcgataa ctgtgattga tcagaccctc
     6961 ttcgattctt agcacctccc tgcaggattg accagatacc tgcagaaagc agttagcgat
     7021 cgtcatcagt tcgttgctat ctcctacatc cattttgttg gatatttacc attcaacact
     7081 gcctgcagga tagtttctct tacctgcatc agatctgcag gatgtgtaag cccgcaccag
     7141 ggcccatcac attttctgac ttgtacaaag agccctttat tcatttctac actcaccgca
     7201 tcaggcagaa agaactccac atcgggatca gcaccgtgct gtttgctgaa tgtctgaaag
     7261 gacacatcaa aacccgtcaa tacagaagca ggcaacaacc aggcattcat agataccaac
     7321 gctgatccat taagtatcct ggttccggac atgatctgtc cgttcttcct tgcgatccct
     7381 ttgtactcgg caatctttaa caggttacct tcttcatcaa cggtacacag cccgcgggat
     7441 acttctccgt tctccgacaa tgtatcacgc aatcgatacg ccagcagaaa gccctgctca
     7501 ttcgtgacct gatttgcatg ctgtactgct ttcccataca tatcagctcc atacagatca
     7561 tccgcattta ctaccagaaa ggaacctgaa acaagcgctg ctgcactcaa gacggcatgt
     7621 ccggttcccc agggtctgtt tctatcagat ggatagcatg ccgactgaga tacatataaa
     7681 atctccatcc tatttttaaa gaatgaaagt ctttctgtca acagaaccgg aacctgatcc
     7741 cttactacta tgatcaccct tcttataccg gcctgcaagg catcaaacag gttgtactcc
     7801 aatagcaatt caccggccgg gccaacagcc tcctcttgtt tgctgctgcc ataccggctg
     7861 gataacccac ctgccagaac taata
//
LOCUS       DAOMAX010000115         7784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1401_length_7784_cov_3.736706, whole genome shotgun sequence.
ACCESSION   DAOMAX010000115 DAOMAX010000000
VERSION     DAOMAX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_7784_cov_3.736706"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PK742_07315"
     CDS             complement(<1..576)
                     /locus_tag="PK742_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQU76437.1"
                     /translation="MSRRKERQNWKWIAAALFIIVAGAAWYGMRYYRYLYHKPTAVSD
                     SSLILYIPTGADFEQVLDSLRQLGMAQDEKALKWVAGVKKYSDHVKPGRYAVEPHWSN
                     DELISHLRSGNQAPVRVVIQEVRTLPDLAKLLDKYLEPETSDFLSALQSDSLQQAYGL
                     DEQTMLTAFIPNTYEFFWNTSPVRALDRLHKE"
     gene            complement(658..996)
                     /locus_tag="PK742_07320"
     CDS             complement(658..996)
                     /locus_tag="PK742_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76438.1"
                     /translation="MKTMSVIFTCLLTATTAFAGNEPDICNRQSYPKEIASLLTSEKP
                     FFEEDLSGVVTIRFTVDQDKAIHIVEVESTNLFLKSHALEALEGKQMETECLPDQEVY
                     EITVKFNYAA"
     gene            complement(1183..1521)
                     /locus_tag="PK742_07325"
     CDS             complement(1183..1521)
                     /locus_tag="PK742_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76439.1"
                     /translation="MKTLVLTTLMSVLSLNVFASGEQDKTEERSELASQVRDMVVNED
                     LFDVMQYSGELSIQFTVDENNQLHVTEVSTDDFSLEYHVRRTLDNVKVAATNTMVGKT
                     ISLVIDLVQH"
     gene            complement(1621..2865)
                     /locus_tag="PK742_07330"
     CDS             complement(1621..2865)
                     /locus_tag="PK742_07330"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQU76440.1"
                     /translation="MSVSGWRQLVGIFHQYTRLAWISFLGATLLCLPFHKIFPVFPLL
                     LTGLFALLSGLSGPCIRSFQENKRMLLGLLLYIPALYAWYISPDKNLAKTDLQVKFSL
                     WIFPVLLAMNPRLSAKQINRLLQAFASATVLFVLTSLVYATWQYLHQGYDAFSYKKLV
                     AFTIIHPSYIGMFIDMTIALLLLDMFQPIHDRRWLPRKWTLVIVLLLFVFLILLTAKN
                     AMLLGMLLLVIALFNYVRTTGKWKQAALASLILCSLLSLFIAFNPTARERFTILLRYN
                     DVSYENSVNSRAETWKAVSQLIPQYGWKGAGSGELQGLLDRQYEQNGFDLGVKEHHNA
                     HNQYFQTLLESGIGGLLLYLLCFGTGIWWAVKNKDRMLGAFLLVFLFSILTESMLKTQ
                     SGVLFFTLFHAIFLMQLPVKRD"
     gene            complement(2875..4221)
                     /locus_tag="PK742_07335"
     CDS             complement(2875..4221)
                     /locus_tag="PK742_07335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQU76441.1"
                     /translation="MCLCLFLYLRFFHPTGFQDYYSLLDNALWALVIVVLWYFYASVF
                     DLYKLSLVDKQGDILKNTIITATLTGITYLFIPFFSPTLPLSRLPAFLLIITMVISLL
                     GWRLLYAALFKHPILPVRAIVVGAGYTGRELVKTLLHDEQIYHRTAYQIFGFIDDDTH
                     KLQKEYDGVKVLSGSDKLVRYVNRLHLDEVILAIPEHEILFPEMQMALIRLEEQGTRV
                     RFAYDIYEQLTGRVMVKFKNGQYFLANPYSINQKKSFYRVANRMLNIVWGVLACLPFV
                     LLIPLVWAINLFSSRGPLFYSQERVGQNGKGFRILKFRTMIVDAEKDGTARFASAGDD
                     RITPAGRFLRKARLDELPQFVNILKGDMNLIGPRPERQVFVDELSHTIPFFRLRNLVK
                     PGLTGWAQVRHHYTTATEDSLVKLQYDLFYIRHRSLMLDLRIMFSTLPVIFKMKGV"
     gene            complement(4330..5277)
                     /locus_tag="PK742_07340"
     CDS             complement(4330..5277)
                     /locus_tag="PK742_07340"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HQU76442.1"
                     /translation="MVDFTRLKNRLNAWLFIAAVYFIIWSALVLIFPQAMHSIVVETS
                     QTNLVFWDLIAIVTIILGLGLLVAAFDPFRNWLIMFLNLLFHVGIIVGFVVGWFNGIF
                     TSNFLPFLFFNHFIWLIPLVTGIYLVYKRGYQADKILIETFNESDFPLELFETVSGKN
                     LQELSEEQPVLLVFLRHFGCPFCQETLLDVRSRRAELEAKGFTIVLVYMVSPAIGQEY
                     LEQYGMDDMEQLSDPESIAYKRFRLNRGNFRQLLGPKVLYRWAWLGVKKRIFFTRVAG
                     DINQMPGLFLLRNGQVVRQYVHRTSADKPDYSIFLSYSE"
     gene            complement(5313..6269)
                     /locus_tag="PK742_07345"
     CDS             complement(5313..6269)
                     /locus_tag="PK742_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HQU76443.1"
                     /translation="MDYVLIIAGFILVIKGSDWLVDGASSLAKRLNISDLVIGLTVVA
                     FGTSAPELAINIFASANESTELAIGNVLGSNLFNILVILGIAAIIRPMTVHSNTVYKE
                     IPLSLLAALMLGVVANDHLLDGSIDSVISRSDGLVLLGFFIIFLYYTYIISQQSSNTE
                     GEFVQTSPLWKSIGFTTIGLAGLLLGGKILVDGAVGVARGWGVSESVIGLTILAIGSS
                     LPELATSIVAARKGKSDIVIGNVVGSNIFNIFLVLGVSATIKPLPFQVSSNMDESMVV
                     GITIILFFFIYNHKENQLVRAHGITFLILYAVYMTYLLVAKA"
     gene            6346..7194
                     /locus_tag="PK742_07350"
     CDS             6346..7194
                     /locus_tag="PK742_07350"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HQU76444.1"
                     /translation="MKRLELRLPLLIGATILAGVLQAQQLPMPSPYAEVEQTIGLAEI
                     EIEYSRPSMKGRTIFGDLVPYGEIWRTGANKCVQLEVSDPIMVNGNMLDSGKYSLFTI
                     PGEKEWTIIINTNTELWGAGGYSQEQDVMRFNVPSSGMAETAESFTIDFSNITPNTAD
                     CNIYWERTRVSFPIEHDYREKAMANIKEALSKEDAPFGAWESAAEFYIDHDLDKKKAL
                     EMAKKSVELKEVFWNVYTLSRAFDYNGMRDEALQAARRSLELAEEAQYKTYIDLNKKN
                     IEAWSK"
     gene            7275..>7784
                     /locus_tag="PK742_07355"
     CDS             7275..>7784
                     /locus_tag="PK742_07355"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU76445.1"
                     /translation="MRYTLFIISLLTWLSSIAQNGIITGTISDAASNEPIPFATISLQ
                     GSTTGTASDIDGHYSLEGLEPGLYNIEIRSVGYRSQTAYEIQVTNVKPVVADFRLETA
                     TEVLDAVVIEAGPNDKTAESPVSLRTIGVNEIKRNPGANRDISLVIQSLPGVASTASF
                     RNDILIRGGA"
BASE COUNT         2204 a         1895 c         1804 g         1881 t
ORIGIN      
        1 ttccttatgc agtctgtcaa gagccctgac cggagaggta ttccagaaaa actcgtaggt
       61 atttgggata aaggctgtca gcatggtctg ttcatccaag ccatatgcct gttgcaggga
      121 gtcactttgt aaggccgata aaaaatctga ggtttccggt tccaggtact tatccagcag
      181 ctttgccaga tccgggagtg ttcgcacctc ctgtataact accctgaccg gtgcctgatt
      241 gccgcttcgc aggtggctaa tcagttcatc gttactccaa tgtggttcca ccgcatatct
      301 gcccggcttc acatgatctg aatatttttt tactccggcc acccatttga gtgctttttc
      361 atcctgtgcc ataccgagtt gccggagaga atcaagcacc tgttcaaaat cggcaccggt
      421 tggtatatag agtataagtg aagaatcact aacggcagtt ggtttatggt agaggtaacg
      481 gtaatagcgc ataccatacc acgcggcacc tgccacaata atgaacaggg cagcagcgat
      541 ccatttccag ttttgtcgtt ctttgcggcg agacatgatg cggccaaaaa taacgcaaaa
      601 aacaagcccc gcattgcggg gcctgcttcg ttaaccactt aaccgccttg aagtatatta
      661 tgctgcgtag ttgaatttca cagtgatctc atagacctcc tgatccggca gacattccgt
      721 ttccatttgt tttccttcaa gggcttccag agcatggctc ttcagaaaca gattggtgga
      781 ctctacttcc acaatatgga tggccttgtc ctggtcaacc gtaaagcgta tggttaccac
      841 tcctgacaga tcctcttcaa aaaatggctt ctcacttgta agcagacttg ctatctcctt
      901 cggatacgat tgccggttac atatatccgg ttcgtttcct gcaaaagctg tagttgcggt
      961 gagcaggcaa gtaaatatga cagacatcgt tttcatagta tgttgtttgt tggtgaaaca
     1021 aaggaacttg ttaggttctg aaggttttcc taaggcagca ttaatatctg accgtatcct
     1081 gttaaacact gtttctgata gttaatgacc acagacacct gttaaatagt ttaaaaagga
     1141 tgccctgcct ggcagggcat ccgggtgcag ccagatgaca acttagtgct gcaccagatc
     1201 gatcacgagg ctgatggtct tacctaccat ggtgttggta gcggccacct ttacgttgtc
     1261 cagtgttcgt ctcacatggt actccaggct gaagtcatca gtagaaactt cggtcacatg
     1321 cagctggttg ttttcatcaa cggtgaactg gatactcagc tcaccgctgt actgcatcac
     1381 atcaaaaagg tcttcgttga ccaccatgtc gcgaacctgg ctggccagtt cactgcgttc
     1441 ttcggtttta tcctgttcgc cggaagcaaa aacattcaga ctcaggacac tcatcagggt
     1501 tgtaagtaca agcgttttca ttgtattgat tttttggttt ttcctgttgg ttacaaccat
     1561 gagtcgcaca ggccccgcag tttgttacag caggatgaaa agaaaatccg ttaataagtg
     1621 ttaatcccgt tttacgggca gctgcatcag gaaaatggca tgaaacaaag tgaagaaaag
     1681 cactccgctc tgggtcttca gcatagattc tgtaagtatg ctgaacagga acaccagaag
     1741 aaatgctccc agcatccggt ccttattttt aacagcccac cagatgcctg tgccgaaaca
     1801 gagcaggtac aggagcagtc cgccgatgcc gctttccagc aaggtttgaa aatactggtt
     1861 atgtgcattg tgatgttctt taacccccag atcgaacccg ttttgctcat attgccggtc
     1921 caacaatccc tgcagctcac cacttcctgc acctttccat ccgtattgtg gtatcaattg
     1981 tgagaccgct ttccaggtct ctgcccgtga gttgacactg ttctcatagc tgacatcatt
     2041 atacctcaag agaatggtaa agcgctctct tgcggtagga ttgaaagcaa tgaacagcga
     2101 cagcagcgaa cacaatatga gagatgccaa tgctgcctgt ttccattttc ctgttgttct
     2161 tacataattg aataaggcga tgaccagcag cagcattccg agcagcatgg cgttcttggc
     2221 agtaagcaga atgagaaaaa caaacaacaa aagcacaatg accagggtcc acttccgggg
     2281 taaccaacgc ctgtcatgaa taggttgaaa catatccagc aacagcagag cgatggtcat
     2341 atctatgaac attcctatat atgatggatg tatgatggta aaagccacca gctttttata
     2401 gctgaatgca tcataaccct gatgcaggta ctgccaggtg gcataaacga ggctggtcag
     2461 tacgaatagc acagtagctg aggcaaacgc ctgcagcaac ctgttgatct gcttcgcact
     2521 aaggcgggga ttcatagcca gaagaaccgg aaagatccaa agagaaaatt tcacctgcag
     2581 gtctgtcttc gccaggttct tatcaggaga aatataccag gcatataagg caggtatgta
     2641 cagcagtaag cccagcaaca tgcgtttgtt ttcctgaaag gatctgatgc agggaccgct
     2701 gagacctgac agcaatgcaa agagtcctgt aagtagcagc gggaaaacgg gaaatatttt
     2761 atgaaatgga agacacagca gcgtagctcc caggaagctg atccaggcaa ggcgggtgta
     2821 ctgatgaaag ataccgacaa gttgtcgcca tccggatacg ctcatgtgct aaagttacac
     2881 acctttcatc ttgaatatga cgggaagcgt ggagaacatg atcctgaggt caagcatcaa
     2941 actccggtgt ctgatgtaga acagatcata ctgcagtttg accagcgaat cttcagtagc
     3001 agttgtgtag tgatgtctga cctgcgccca gccggtaagt cccggtttta ccagattgcg
     3061 aagccggaag aatgggatgg tatgcgacag ttcatcaaca aacacctgac gctcaggtct
     3121 ggggcctatc aggttcatat cacctttcag gatattcacg aactggggta gttcatccag
     3181 cctggccttg cgcagaaatc tgcccgcagg agtgatgcga tcatcgccgg cacttgcaaa
     3241 cctggcggtt ccgtctttct cggcatccac gatcatggtt cgaaacttca ggatcctgaa
     3301 acccttacca ttctgcccta ccctttcctg actgtaaaac agagggcctc tgctgctgaa
     3361 caaattgatg gcccacacca gcggtatgag caaaacaaaa ggcaggcagg ccagcacccc
     3421 ccaaacaata ttcagcattc tgttggctac ccggtaaaag ctctttttct ggttgatgct
     3481 ataaggatta gccagaaaat attgtccgtt cttgaacttc accatgaccc tgccggtaag
     3541 ctgttcataa atgtcatagg caaagcgcac gcgggttccc tgctcttcca gccgtatcag
     3601 tgccatctgc atttcaggaa acaggatctc gtgttcgggt attgccagga tcacctcatc
     3661 caggtgcagg cggttcacat acctgacaag cttatcagac cctgacaaga ccttcacacc
     3721 atcatattcc ttctgaagtt tgtgtgtatc atcatctata aaaccgaaga tctgataggc
     3781 tgttctgtga tagatctgtt catcatgcag caaggttttt accagctccc tccctgtata
     3841 tccggcgcca accacaatgg ccctgacggg taaaatagga tgcttgaaca gtgcggcata
     3901 gagtaatcgc cagcccagca atgagatgac catagtgatg ataagcagga aagcaggcaa
     3961 acgactaagg ggcaaggtcg ggctgaaaaa aggaatgaac aaataggtga tacctgtcag
     4021 ggtagcggtg atgatggtgt tcttcagaat atcaccctgt ttatcaacca acgataactt
     4081 atacaggtcg aaaacgctgg catagaaata ccacagcacc acaatgacca gagcccaaag
     4141 ggcattatct aacaaagaat aataatcctg gaatccggta ggatgaaaaa aacgcaggta
     4201 caggaacagg cagaggcaaa tgagcagggt atctccggca atcagcgtaa aacgccggag
     4261 aaaaccacga aagtcatatg tactgttgga agccaggcca caaaaatagc caatcgtgaa
     4321 acaggagtct tattcgctgt aggacagaaa aatgctgtaa tcaggtttat ctgccgaggt
     4381 ccgatggaca tactgcctca ctacctgccc gttacgaagc aggaacagac ccggcatctg
     4441 gttgatatct cctgctacac gggtaaagaa tattcgcttt ttaacaccca gccaggccca
     4501 tctgtaaagc actttgggac caaggagctg gcggaaattt cccctgttca agcggaatct
     4561 tttataggca atactttcag gatcactcag ctgctccata tcatccatac catattgttc
     4621 cagatattcc tggccgatag ccggcgatac catataaacg agaacaatgg taaagccttt
     4681 tgcttccagt tctgctctgc gtgacctgac atcaagaagt gtttcctgac agaacggaca
     4741 tccaaaatga cgcagaaaaa cgagcaagac cggctgttct tcgctgagtt cctgcaggtt
     4801 cttaccagaa accgtttcaa acaactccag cgggaaatct gattcattaa aagtttctat
     4861 cagaatttta tctgcctggt agcctcgctt atacaccaga tagatacccg ttaccagtgg
     4921 gatcagccat ataaagtgat tgaaaaacaa aaatggcagg aaattactgg tgaagatccc
     4981 gttgaaccat cccaccacaa aacctacaat gatgcccaca tgaaacagca gattcaggaa
     5041 catgatgagc cagttgcgga atggatcaaa agccgccacc aacagtccca gacccagaat
     5101 gatggtcaca atggcgatca gatcccagaa aaccagattg gtttgtgagg tttccactac
     5161 aatgctgtgc atagcctgcg ggaaaataag caccagggca ctccatataa taaagtacac
     5221 cgctgctatg aacagccagg cattgagccg gttcttaagt ctggtaaaat ctaccatgat
     5281 catatgcctc aacaagccaa aatcaggatt gttcaggcct tagctacaag taaataggtc
     5341 atatacacag catacaggat caaaaaagtg atcccatgag cacgaacaag ctggttttcc
     5401 ttatggttgt aaataaaaaa gaagaggatg atggtgatac caaccaccat agattcatcc
     5461 atgttagagc tgacctgaaa gggtaatggt ttgatggtgg cactcactcc aagtaccaga
     5521 aagatgttga aaatattgct gcctactaca tttccgatca cgatatcact cttccctttt
     5581 ctggcagcta cgatgctggt agccagttcg ggcaaagatg acccgatggc caggatggtg
     5641 agaccaatca ctgattcgct tacaccccag cctcttgcca cacctactgc tccgtcaacc
     5701 aggatcttcc cccccagcag cagtccggcc aatccaatgg ttgtgaaacc gatgcttttc
     5761 cagagtggtg acgtttgcac gaactctcct tctgtgttgg atgattgttg tgagatgatg
     5821 taggtataat agagaaaaat gataaagaaa ccgagtaaga ccagcccgtc acttctggat
     5881 atgaccgaat caatactgcc gtccagcaaa tgatcatttg ccactacacc cagcataagc
     5941 gcagccagaa gactcagggg tatctcttta taaactgtgt tgctgtgcac ggtcatgggc
     6001 cgtatgatcg cagcaatacc cagtatcaca aggatattaa acaggttact tcccagcaca
     6061 ttgcctatgg ccagttcagt actttcattg gcagacgcaa aaatattgat ggccagctca
     6121 ggcgcactcg ttccgaatgc cactacggtc agtccgatca ccagatctga tatattcagc
     6181 cgcttggcca gagaggaagc tccgtccacc agccagtctg agcctttgat caccagaatg
     6241 aacccggcta taattagtac ataatccatg tatttggtgg gtttgagccg ttctgtaaac
     6301 cctataaggg ctgtgttgtt acctttgcaa ataaaaccaa ttatcatgaa aagacttgaa
     6361 ctgcgtctgc cgctgctgat aggagcgacc attctggcag gtgtgctgca ggcacagcaa
     6421 ttgcccatgc ccagtcccta tgctgaagta gagcaaacta taggtttagc agaaatagag
     6481 atcgaatatt cccgtccatc catgaaaggt cggaccatat ttggtgacct tgttccttat
     6541 ggtgaaatct ggcgcacagg agctaataag tgtgtacaac tggaagtgag tgatccgatc
     6601 atggtgaacg gaaatatgct ggactcagga aagtacagtc tgttcactat tccgggagaa
     6661 aaagaatgga ccattatcat caacaccaac accgaactat ggggagcagg cggatatagc
     6721 caggagcagg atgtgatgcg tttcaacgtg ccctcttccg gcatggctga aaccgcagag
     6781 tcttttacca ttgacttcag caacatcact cccaatactg ctgactgcaa catatactgg
     6841 gaaagaaccc gggtatcatt cccgatcgaa cacgattacc gcgaaaaggc catggccaat
     6901 atcaaggaag cccttagcaa ggaagatgca cctttcggag cctgggaatc tgccgcagag
     6961 ttttacatag accatgatct ggacaagaaa aaagcactgg agatggcgaa aaagtcggtc
     7021 gaactgaaag aggtcttctg gaacgtatat acgctgtcaa gggcctttga ctacaatgga
     7081 atgcgtgacg aggcactgca agccgcccgc cgatcactgg aacttgctga agaagcccag
     7141 tacaagacct atatagacct gaacaagaag aatattgaag cctggtccaa gtgatccggc
     7201 agcacaaact aaaaaagccg gcgtttgtcc ggcttttttt ttgaacaaaa caggacagac
     7261 cctgttccta ccccatgcgt tataccctct tcataatcag cctgttgacg tggctgagtt
     7321 ccatcgcaca aaacggcatc attacaggta ccatttcaga cgctgccagc aatgagccca
     7381 ttccatttgc caccatctct ttacagggca gcaccacagg tactgccagt gatatagacg
     7441 ggcactacag cctggaaggt ctggagcccg gactgtacaa catagaaata cgctcagttg
     7501 gataccggtc tcaaacagcc tacgaaattc aggttaccaa tgtcaaaccc gttgtggcag
     7561 actttcggct tgaaacagcg acagaagttc tggacgctgt ggtcattgaa gccggaccga
     7621 acgacaagac agcagaaagt ccggtatccc tgcgcaccat cggcgtcaat gagatcaaaa
     7681 gaaatcctgg tgcaaacaga gatatttcgc tggtcattca gagtttgcca ggggtggcca
     7741 gtacggcatc cttccgcaac gatatcctga tcagaggagg tgct
//
LOCUS       DAOMAX010000116         7713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1421_length_7713_cov_4.781797, whole genome shotgun sequence.
ACCESSION   DAOMAX010000116 DAOMAX010000000
VERSION     DAOMAX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1421_length_7713_cov_4.781797"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1761)
                     /locus_tag="PK742_07360"
     CDS             complement(151..1761)
                     /locus_tag="PK742_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76446.1"
                     /translation="MKIKLIILMLCCSAFAWNKAQANYGDEVFARILLAVNRDLPTPP
                     HLVWVSNMNGVASTYANGTIEVDSRLLERMRGFGADSANALAHILAHEVMHYYMEHFW
                     AGEFALPYGDASWGQELQGIDSDTLQLQYQETQADLYAIFYATSAGYQTGRIADAVID
                     SIYRWYNLPFKMKRYPSLDARKEIARTAARDVAALLPMFEAGKMMLMLGMSASGKEQL
                     TYLRGAAYAFQHITDNNIRTTEMYNNMAVVRIMQAITMLNDTIAAFRWPLVIESGSVL
                     YDAQSTRGAGMELPEEAARLLQEAKNLLESALEMDAAYQPAMTNLCVALMLLGKQGSM
                     ADVLDELTELSDNTALELELRGLAAWLKGKHDQAVELLEEADAAGSLTAKRNAEVAAG
                     KLMPVGIDLPVLADISETIDGTLLPDYFSGISALDGSRTDERSQNLLLFCDTIGRYVI
                     FEVKFKFQDRAFRTLKMVKTLPGDLPPHDKGISQRSDVRDLQKQYPEDATILLESDHE
                     VFVFPLSYALVWISQNGKVDGWGWYWAQ"
     gene            complement(1803..3767)
                     /locus_tag="PK742_07365"
     CDS             complement(1803..3767)
                     /locus_tag="PK742_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQU76447.1"
                     /translation="MKTKHLLTLTLGLLLVSSTAFAQYGTGLFYDGKAAKEFSYKKPG
                     KGYAGELPSSFSLKAHTPYAKNQGNYGTCTSWAVAYSAMSTAYAYNMGITNRNIITGI
                     AFCPYFVYNQVKADASCSSGSSIPLSILLLMETGAKRFYSPVIGCGALISDDMINDAG
                     NFRIDDAYILYDEGAGIPADMTAAALTTYMKSKATPDYTDIKAAITFGTPVIFGSFIP
                     ASFFAARSSFWEPTYDEKQNPGQAVLSNEGMHQMHAMTIIGYDDNKYGGAFEVMNSWG
                     EAFGNKGFVWVKYADWALFCYEAYALELGGLGDAEMNNVSGCVSGDCDNGYGIYKFAS
                     GDRYEGHFSGRNFNGYGIYTWASGEVYAGQWSMNKRQGEGTRYLPNGEYGTCIYDNDK
                     LISGFSNWSYTNGDTYYGALAQDFSRIGFGRYTFSNGSVYEGSWKNNVREGLGRMTYT
                     NGDIYVGEWSDDSPNGVGMLIHTNGKVDAGSWSYGKMMSGQSYGYATDQSLLAVRELS
                     LPEPSALAYASADCTDGDCLYGKGKRVYKGGATYEGEFKDGVEDGFGTMTYGDGTKVK
                     SNWKQGYNFGVARYDYGDGTMALAEFKGGTLNGYVLVMKSGNMVVQYYEDGVYLRQVN
                     PTYSPGTSVMNGDLSNNAPSSGSSIEVPGK"
     gene            3847..6993
                     /locus_tag="PK742_07370"
     CDS             3847..6993
                     /locus_tag="PK742_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQU76448.1"
                     /translation="MRLSGLMILSILLSTIVSSLWAQEYPEEVTPCLDKLTAGEMEAA
                     YDLCITCNRAAMAHEGLDPVVVMNGLIETSNQYYFAGYLDAAMVFNQMALDHADSLQL
                     PHDENYASYLLNLFAIAFQSGNKEIVLRKGQETAALILQFAGNTPELVYVHTAMGVAY
                     LADGQLAESEENFELASAYQLELAGMPDSTYFNIQLQLSDVYEAGGDLNKAIQHTQKV
                     LTGIKEAGLQNEALTADGTLNLFYLAFVSGSTEMVLQKGPETARLLEKVYGSTPDLVW
                     VYTVLGTEYLLRSQLAESEESFELASAHQLIVTGAPDSTYFSLQLRLSEVYQLYGDHV
                     KAIEKVEEVMAEMEAAGMHNSALLADSYDLMMLAATELNDEAALVEYVNGLMEVIGEL
                     PIDVMASKYFNMVIAINRFDIANGTRVITEYGLDTITFQVLEAVGKLDIDPMILSNGY
                     LVLGNIYLMDGLYDKVYVNYDKASSLVAERYGKDFLYITYRNTMAICAEKQGQPELAK
                     SIYEDNFQLTERIIQNNFAYLPEQAQAQLIQNMGFVRTCFASFTMRYAEVYPDMLAAL
                     SEEALLFQGAVLRNASGIRNRLLTGGDPQDAELVDNWLRMKQQAAAVRFSNPDQADAL
                     DKQAEDLEKKFSLGIKRKSSEQEALHWSDLQNEMLAGTAVVQFLRIESDGYFRTGPAQ
                     YCALVTRSGLERPELITLCDEEQLASLLSARENEPAGDHVRRLYLYPDPLFPEDTTDY
                     QGDRLYQMIWQPLEASLTGTDTIHFAPVGLLHRIAFQALSDGDSLLLQRYVMLQEKDP
                     GMPPSSFESVRSILAVGGIDYGLSETAVAVADDRGSGWSPLPGTRQEVDMIANMAEAK
                     QISCTVLTDGKAREDILTDQQLLSADVLHLGTHGFFLPAEPNPVPEAGDPGYQFRTAE
                     SPLMRSGLLLSGGNTSWIQGSTGNVDGILTASEISNLNMLDIELVVLSACETGLGDIQ
                     GDEGVYGLQRAFAMAGAQNLIMSLWKVPDRYTAELMTRFYTYLLEPSDPATALRRAQL
                     DLSTRTDVYNWAAFVLVR"
     gene            complement(6990..7388)
                     /locus_tag="PK742_07375"
     CDS             complement(6990..7388)
                     /locus_tag="PK742_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76449.1"
                     /translation="MDFEQKKLQELHADINKWKELVELVRDETRIFEHQLEQILQAGP
                     SREVMPYIQKFESMFIRQNEVSDELFHDLKAADHELKQMIDEDPAVLNVPLPDHDHLR
                     DKVNTYENLFLELRNEFRHFHLMASHPEAH"
     gene            complement(7533..>7713)
                     /locus_tag="PK742_07380"
     CDS             complement(7533..>7713)
                     /locus_tag="PK742_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76450.1"
                     /translation="SGDDDGDDSGDDDGDDHGDDHGGGDDDGDDDDDDHGGGDDDDDE
                     DDDDGGGSDDDGDDD"
BASE COUNT         1926 a         1881 c         1917 g         1989 t
ORIGIN      
        1 cattttctac gttcatgatg caaagctatg acatactatg gtactatgca atacctaata
       61 ctatgttttt acaagtattt ggcgttaaaa acagttaagg ccaccaaaag gtggccttaa
      121 ctgtatgata atcaatgtat aagggaaatg ttattgggcc cagtaccaac cccatccatc
      181 cacttttccg ttttggctga tccagacgag agcataagaa agggggaaaa cgaacacctc
      241 atgatcgctc tcaagcagaa tggtggcatc ttcggggtat tgtttctgca ggtctctgac
      301 atcagacctt tggctgatac ccttatcatg cgggggaaga tcgcctggaa gggtcttcac
      361 cattttaagg gtgcggaagg ccctatcctg gaatttgaat ttcacttcga agatcacata
      421 acggccgatg gtatcgcaga acaacaacaa attctggcta cgttcatcgg ttcgtgatcc
      481 atcgagtgca gagatccctg aaaagtaatc aggtaacaac gtgccgtcta tggtctcgga
      541 aatatctgcc agcacaggca ggtctattcc taccggcatc agtttgccgg cggccacttc
      601 tgcatttcgt tttgcggtca gactgccggc agcatctgct tcttccaaca actccaccgc
      661 ctggtcgtgt ttgcctttca gccaggcagc cagtccgcgg agctcgagtt ccagtgctgt
      721 attatccgac agttctgtca gttcatccag cacatcggcc atacttccct gtttacccag
      781 cagcatcagc gccacacaga gattggtcat ggcgggctgg taggcagcgt ccatttccag
      841 tgcagactcc agcaggttct ttgcttcctg caagaggcgg gctgcttctt cgggcagttc
      901 cataccagca ccacgtgtgg actgggcatc atacaataca gaaccacttt caatcaccag
      961 aggccatctg aatgcagcaa tggtatcatt gagcatggta atggcctgca tgatgcgtac
     1021 cacggccatg ttattataca tttctgtggt tcgtatatta ttgtcagtga tatgctggaa
     1081 ggcataggcc gcacctctca ggtaggtaag ttgttccttc ccggaggccg acatgcccag
     1141 cataagcatc atcttgccgg cttcaaacat gggcagcagg gcggccacat ctcgtgctgc
     1201 agtgcgtgcg atctcttttc tggcatccag agaaggatag cgtttcattt tgaagggcag
     1261 gttgtaccag cggtagatgc tgtctatgac cgcatcggca attctgcctg tctggtagcc
     1321 agcagaagtt gcatagaaaa tggcgtatag gtctgcctgt gtttcctgat actgcagttg
     1381 cagggtatcg ctgtctatgc cttgcagttc ctgaccccag gaagcatcgc cgtatggcag
     1441 ggcgaattca cctgcccaga aatgctccat gtaatagtgc atcacttcat gggcgaggat
     1501 atgtgccagg gcgttggcag aatctgcacc aaaacctctc attcgctcca gcagtctgct
     1561 gtccacttct attgtaccgt tggcataggt ggaagccacg ccgttcatgt tgctgaccca
     1621 gaccaggtgt ggtggtgttg gcaggtctct gttcactgcc aggagaatgc gggcaaagac
     1681 ctcatcgccg tagttggcct gcgctttgtt ccaggcaaaa gcggaacagc aaagcatcag
     1741 tatgatcaat ttgattttca tggctttgct gttccgtttg cttgaatgaa tatgtttgac
     1801 tgttatttac cgggcacttc gatagaagaa cctgatgaag gggcgttgtt gctcagatcg
     1861 ccgttcatga cagaggtacc gggagaatag gtcggattga cctggcgcag atatacgccg
     1921 tcttcgtagt actgaactac catgtttccg gatttcatga ccagcacata accgttcagg
     1981 gtaccacctt tgaattcggc cagggccata gttccatcgc cataatcata tcttgccaca
     2041 ccaaagttgt aaccctgttt ccagttgctc ttcaccttgg taccatcgcc ataggtcatg
     2101 gttccgaaac catcttccac accatccttg aattcacctt cgtaggttgc accaccttta
     2161 taaacccgct taccttttcc gtacagacaa tcgccatcgg tacaatcggc agaagcgtat
     2221 gcaagtgcag aaggttcggg caggctgagt tcacgaacag cgaggagaga ctgatcagtg
     2281 gcatacccgt atgactggcc actcatcatt tttccgtaag accagctgcc ggcatccact
     2341 ttaccattgg tatggatgag catgcctaca ccgttgggag aatcatcgct ccattcgccc
     2401 acatagatat cgccgttggt ataggtcatt cttcccagac cttcgcgaac attgttcttc
     2461 cagctgcctt cgtaaacgga gccattgctg aaggtatagc gaccgaaacc tatgcggctg
     2521 aagtcctgtg ccagtgcacc gtagtaggta tcaccattgg tatatgacca gttggaaaaa
     2581 ccgcttatca gtttatcatt gtcatagata caggttccgt attcaccgtt gggcagatac
     2641 cgggttcctt caccctgacg cttgttcatg ctccactgtc cggcatatac ttcgccactg
     2701 gcccaggtat agatgccata tccgttgaaa tttcttccac tgaagtgacc ttcgtaacga
     2761 tcgccgctgg caaacttgta aatgccatag ccgttgtcac aatcgccact gacacaacca
     2821 gaaacattgt tcatttctgc atcacccagt ccacccagtt cgagtgcata agcttcgtag
     2881 cagaacaagg cccagtcagc atatttcacc cacacaaaac ctttgttgcc aaaagcttca
     2941 ccccagctgt tcatgacttc aaacgcacca ccgtatttat tgtcgtcgta accgatgatg
     3001 gtcatggcgt gcatctggtg catcccttca ttgctgagta cagcctgacc gggattttgt
     3061 ttttcatcat aggtaggttc ccagaaggaa gagcgggcag caaagaaaga ggcaggaata
     3121 aaagaaccga aaatgacagg ggttccgaaa gtgatggctg ctttgatatc ggtataatca
     3181 ggagtggctt tgctcttcat ataagtggta agggcagctg cggtcatgtc ggcaggaata
     3241 cccgcacctt cgtcatacag gatataggca tcatctatcc tgaagtttcc tgcatcattg
     3301 atcatgtcat cagagatgag ggccccgcaa ccgatcaccg gagaataaaa gcgtttggca
     3361 ccggtttcca tcagcaacag aatagacaaa ggaatggaac ttccactgga gcaggaagca
     3421 tcagccttca cctggttata gacgaagtaa ggacagaatg cgataccggt tatgatgttc
     3481 ctgttggtga tacccatgtt ataggcatag gcagtactca tagcagaata tgctacggcc
     3541 caggaagtac aggtaccata gttgccctgg ttcttggcat agggtgtatg ggctttcaga
     3601 gagaagctgg aaggcagctc gccggcatag cctttaccag gcttcttata ggaaaattct
     3661 ttggcggctt taccgtcata aaaaaggcct gttccatact gggcaaatgc tgtgctgctt
     3721 acaagcagga ggcccaatgt cagagtcaaa aggtgtttcg ttttcatttt gtgttgttta
     3781 acctgtagat ggctgtgttt tgtatttttc acacgaaatt caaagttaaa aaaagttaag
     3841 agcggcatgc gactatccgg tttgatgatc ttaagtattc tcctcagcac aatagtctcc
     3901 tctttatggg cccaggaata tccggaagag gtgaccccct gtctggacaa actgacagca
     3961 ggcgagatgg aagctgctta tgacctgtgt atcacctgta accgggctgc catggcccat
     4021 gaaggtctgg atccggtagt ggtcatgaac gggctgatcg agacctccaa ccagtactat
     4081 tttgccggtt atctggatgc tgctatggtt ttcaatcaga tggctctgga tcatgcagat
     4141 tcactccaac ttcctcatga cgagaattat gccagttatc tgttgaatct gtttgccatt
     4201 gcctttcaga gtggaaataa agagatcgtg ctccggaagg gacaggaaac agcggcactc
     4261 attttacagt ttgccggcaa cacacctgaa ctggtatatg tccatacggc catgggagtt
     4321 gcttatctgg cagatggtca gctggcagaa tctgaagaaa attttgagct ggcttctgca
     4381 tatcagcttg aacttgccgg aatgcctgat tccacctatt tcaatataca gttgcaactg
     4441 tctgatgtat atgaagccgg cggcgatctg aacaaagcca tacagcatac gcaaaaggta
     4501 ttgaccggaa tcaaagaagc cggattgcaa aacgaagccc tgacagcaga tggcaccttg
     4561 aatctgttct accttgcctt tgtaagtggc agtaccgaaa tggtgcttca gaaaggaccg
     4621 gaaacagccc ggttgctgga aaaagtttac ggcagtactc cggatctggt atgggtctat
     4681 actgtattag gcacagagta tctgctgcgc agccagttgg cagaatctga agaaagtttt
     4741 gaactggcct ccgctcatca gctgatcgtt accggtgcac ctgattccac ctatttcagt
     4801 ttgcagttga ggctctctga ggtgtatcag ttgtatggtg atcacgtcaa agccatagaa
     4861 aaggtggaag aggtgatggc agaaatggag gctgccggca tgcataacag tgccctgctg
     4921 gcagacagtt acgatctgat gatgctggca gcaaccgagc tgaatgatga agcagcgctg
     4981 gtggaatatg tgaacggcct gatggaagtg ataggtgaat tacccattga tgtcatggct
     5041 tccaagtatt ttaatatggt catagccatc aaccgtttcg atatcgcgaa tggcacccgg
     5101 gtcattacag aatacggact ggacacgatc acctttcagg tgctggaggc ggtcggtaag
     5161 ctggatattg atccgatgat cctgagcaat ggttatctgg tgctggggaa tatctatctg
     5221 atggatggat tatatgacaa ggtatatgtg aactatgaca aagcttcctc actggtagct
     5281 gaacgatacg gcaaagattt tctgtatatc acctaccgta acaccatggc catttgtgct
     5341 gaaaagcagg ggcaacctga gctggcaaag tccatctatg aagataattt tcaattgacc
     5401 gaacgcatca tacagaacaa ctttgcctat ctgccggaac aggcacaggc gcaactcatc
     5461 cagaacatgg ggtttgtgcg aacctgcttt gcctctttca ctatgcgtta tgcagaggtt
     5521 tatcctgata tgctggcggc tctgtcagaa gaggcgttgt tgtttcaggg agctgtattg
     5581 cgcaatgcat ccggaatacg caacaggtta ctaaccggag gtgacccgca agatgccgaa
     5641 ctggtggaca actggctgcg catgaaacag caggcagctg cagtgcgctt cagcaatcct
     5701 gaccaggccg atgcactgga taaacaggct gaagatctgg aaaagaaatt ttcacttggt
     5761 atcaagcgga aaagctctga acaggaagcc ctgcattggt ctgacctgca gaatgagatg
     5821 ttggccggca cagctgtggt gcagttcctg cggatagaaa gcgatgggta ttttcgtaca
     5881 ggtccggcac agtattgtgc actcgttacc cgatcaggcc tggaaagacc cgaactgata
     5941 accttgtgtg atgaagaaca actggccagc ctgctcagcg caagagagaa tgaaccggca
     6001 ggagatcatg ttcggcggtt gtatctctat cctgatccgc tgtttccgga agatacgaca
     6061 gactatcagg gtgaccgttt ataccagatg atctggcaac cactggaagc ttctttgacc
     6121 ggaacagata cgattcattt tgctcctgtg ggattgctgc accgcatcgc atttcaggct
     6181 ttatctgatg gcgatagttt actcctgcag cggtatgtga tgttgcagga aaaggatccc
     6241 ggcatgccgc cctcatcttt tgagtctgta cgttccattc ttgctgtggg cggaattgat
     6301 tacggcttga gcgaaacagc agtggccgtg gccgatgaca gaggaagcgg atggtcgccg
     6361 ctgccaggta ccaggcagga ggttgacatg atagccaaca tggccgaagc aaaacagatc
     6421 agttgtaccg tgctgaccga tggcaaagcc agagaagaca tactgaccga tcagcaacta
     6481 ctttcagcag acgtacttca tctgggcact catggtttct ttttaccggc tgagccaaac
     6541 cctgttcctg aagccggcga cccgggatat cagtttcgca cagcagagag tccgctcatg
     6601 cgcagcggat tgttgttgtc aggtggcaat accagctgga tacagggcag taccggcaat
     6661 gttgatggca tactgaccgc atcagagatc tccaacctca acatgctgga catagaactg
     6721 gtagtgctct ctgcctgcga aacagggttg ggtgatatac agggtgatga aggcgtttac
     6781 ggtttgcagc gagcctttgc tatggcagga gcccaaaacc tgatcatgag tttgtggaag
     6841 gtacctgacc ggtacacagc agaactcatg accagatttt atacctatct gctggaaccc
     6901 agcgatcctg ccacagcact caggcgtgcg caacttgatc tgagcacccg aacggatgtc
     6961 tataactggg cagcttttgt gctggtgcgt tagtgcgctt ccggatgcga tgccatcaga
     7021 tggaagtgcc tgaattcatt gcgcaactcc aggaaaaggt tctcataggt gttgacctta
     7081 tcccgaagat ggtcgtggtc gggtaacggc acattcaata ctgccgggtc ttcatcaatc
     7141 atttgtttca gttcatgatc ggctgctttc agatcatgaa acaattcatc gcttacttca
     7201 ttctgccgta tgaacatact ttcaaatttc tgaatatacg gcatgacctc acgggaaggt
     7261 ccggcttgta agatctgctc cagctgatgt tcaaagatcc tggtttcatc acgcaccagt
     7321 tccaccaatt ctttccactt gttgatgtct gcatgtaatt cctgcagctt tttttgttca
     7381 aagtccatga ctgtaaggtt tacttgtttc aaagttgaac gaataacagt gaggtagtgg
     7441 taattaaagt tgcgtaaaaa ggaaactatt gtcaccaaat ggtacgggca ggccaaaggc
     7501 ctgcccgtac actatgaagc actcaacacc tgttaatcgt cgtcgccgtc atcatcgctt
     7561 ccaccaccat catcgtcatc ttcatcatca tcatcatcgc caccaccatg atcatcgtca
     7621 tcgtcgtctc catcatcatc gccaccacca tggtcatcgc catggtcatc accgtcgtca
     7681 tctccagaat catcgccatc gtcatcaccg ctg
//
LOCUS       DAOMAX010000117         7647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1444_length_7647_cov_4.232086, whole genome shotgun sequence.
ACCESSION   DAOMAX010000117 DAOMAX010000000
VERSION     DAOMAX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1444_length_7647_cov_4.232086"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK742_07385"
     CDS             complement(<1..446)
                     /locus_tag="PK742_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQU76451.1"
                     /translation="MKGLREYVIPFIGLKNGLHHFNFEIGAPFFSHFPDSLIRECGLF
                     VDLAFDKKERLFVLNFDISGTISTECDRCGQALDLPIHGNHTLYVKKGEQPEEEDEEE
                     DITWIADGESELDTSEWIYEFIHLSIPVRKLHPDLADGTPGCNPDIL"
     gene            complement(496..1551)
                     /gene="pdxA"
                     /locus_tag="PK742_07390"
     CDS             complement(496..1551)
                     /gene="pdxA"
                     /locus_tag="PK742_07390"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQU76452.1"
                     /translation="MEKKVRIGFTIGDINGIGPEVLIKALSDHRILNYCTPVIFGSNK
                     VLSFYKKLLDAEHFKYAHCDQVSDANPKLINVINTLPEEFIPDPGISSTAGGQAAMLS
                     LEAAADALLDGQLDALVTAPINKHNMHEAGFTFPGHTEYLNHRAGGPGSLMMLLNDSM
                     RVALATHHMPLQEVPGTLSVEYLVSKIRILATSLRQDFGIDKPLIALTGLNPHAGDQG
                     LLGRDELEVIIPAMEQVRKDGIIVSGPFAADGLFGSGQISQFDAVLTMYHDQGLVGFK
                     ALSFDTGINYTAGMPFVRTSPDHGTAYDIAGKNVASATSMLQSIFCALDIYRTRLQYV
                     QNHENPVKRTELAQERG"
     gene            1612..2397
                     /gene="rsmA"
                     /locus_tag="PK742_07395"
     CDS             1612..2397
                     /gene="rsmA"
                     /locus_tag="PK742_07395"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQU76453.1"
                     /translation="MNPAHSFRHKKSLGQHFLRDPDIAERILDAIPAEAADAAVLEIG
                     AGAGVLTRPLFRAFGRKLYVSEIDHRSVRLIATDPGVSADRILEGDFLKCNLQELPTP
                     LYIAGNFPYNISSQIVFRMLEHREKIPALVGMFQREMAVRICASPGNRDYGVISVWTN
                     LFYSGEILFELPPEAFDPPPKVHSAVIRLIKRAEVVPGADDAGVLKLVKMAFQQRRKK
                     LSNALGAVDGARQALEGFGWQDLRPEALEPVQYVQLYQAIVRS"
     gene            2394..3323
                     /locus_tag="PK742_07400"
     CDS             2394..3323
                     /locus_tag="PK742_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQU76454.1"
                     /translation="MKILITDLVHPDLVSLLAERGHQVTSDPAISYKRLSEVIAPFEG
                     LIVSTRLPIDKAIIDAAPNLRFIARVGSGMEHIDMLYAQEKGIRVVSSPEGNANAVAE
                     HALGMLLALLRHIPRAVQEIRQQKWIREANRGIELKGRTIGIIGVGHTGTAFARKLSG
                     MEMRVLGYDKYRNSTDLLYVTPASIQEISEQAEIISFHVPLTAETHYYLNRSFIDNMK
                     NPFILINTSRGKVVSTVDLIDGLSSGKIRGAALDVLENEQFSLYTTKEKEVHEALMAF
                     EDRVLITPHIAGWTKESKYLLSSVLLDKLSDLL"
     gene            3452..7180
                     /locus_tag="PK742_07405"
     CDS             3452..7180
                     /locus_tag="PK742_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQU76455.1"
                     /translation="MRKFFYALVILIVSAGAAMAQKGEIQGKVVDEKGEGIPFANVAV
                     FKDGVLETGAQTDFDGLYSVSGLVPGTYDVEASYQGNKFRITDIIVSSGIIFLDEITL
                     KEGVSLDEVVIVYEAPLVDKGNTSTGGVVTKEDIQRIATRNVTSIAATKEGVYQSDEG
                     GGLNIKGSRGDATEYIVDGVRVSGSLKLPQDAIEQLEVITGGVDPKYGDATGGFVTIT
                     TRGPSKDYNGSVELASSQFLDPYGYNLATLFLTGPIITKNKGTDSASAKLGFFIAAEY
                     EGEKDSDPSAFGNFKLKDDVAASLLDNPLRPAVTGTGFNKNTEFLTNDDFEVVDARQN
                     VASRGLALTSRIDYKLSKTMNFQVGLTYRNFFAHNYVRTFALLNPENNPVSNQNTYRG
                     FVRFTQRFPERKAAEGESSSILGNAYYNIQFDYTKYYGTSMDEDHEMNPFNYGYIGAF
                     DTYRSPVYFYGTDEVSGLNGWYLAGYQDTLVTFDAGTLNPELATYTQTYYDLASAPPG
                     SLFDIQLGSGLLNGDAFVNSTSTYSLWWSRGVPFSSYNKQNDDQYGLNFNAALDLKSN
                     KKSANISKHSIEFGFEFQQRIERFYQVAPIGLWTLARQLANRHIQTLDTDNPILVIDG
                     QQYSYDEYLASGIEFGAYDTIFYNRLFIAEDQSFFDEQLRDKLNELGYGVEQLDFINV
                     DALPLDVLSLDLFSADELLNNGNAFVFYNGYDYLGNQLTTQPSFGDFFTEKDEFGNYT
                     RPIAAFRPVYTAGYIQDRFNFNDIVFRVGVRVDRYDANRSVLQDKYSLYDIYSKGEID
                     GSQNPNGAHPGNIGDDYAVYVDDYNSPSPVILGYRDGDTWFNADGEEVSDPRTIALSS
                     SSGVITPYLVDPDANIKDENFDPSSSFKDYVPQISVMPRVAFSFPISKETNREALFFA
                     HYDILTQRPPTGTSATAYDYYFFQENVNNVFDNPDLRPEKTIDYQIGFQQQLNEVSVL
                     KISAFYRELRDMIQIISVPYAFPVQYTTYGNIDFGTVKGLTVTYDLAKRTKNLKMSTS
                     YTLQFADGTGSSSTSQLNLVGSGQPNLRAIIPLSYDSRHNFKVVLDYRFDDPNGSQPS
                     WLDLVGLNLTMNARSGEPYTRQQNPTPTAQFGVATRANLEGAINGSRLPWHFRTDMRL
                     DKSFGLTMGSGSKERQLNLNAYLWVQNLFDNRNVISVYAFTGNPDDDGYLSSALGQEA
                     IEGQVNAESFIDLYTMKMQNPNNYSIPRRIRLGIGIDF"
     gene            7201..>7647
                     /locus_tag="PK742_07410"
     CDS             7201..>7647
                     /locus_tag="PK742_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76456.1"
                     /translation="MWNKRIIVASAFSLLTVMSVQAKENIGLVGKNAQSYSRLASNCN
                     DATAQIELDINNVRTRILGAGDFWWDLNNGKYVIPKVYPPEIEVSSTFAGAMWIGGID
                     AGGQLKIAAQTYRQSGNDFWPGPLSDAGTTDDAVCTSYDRHWKVNRS"
BASE COUNT         2030 a         1773 c         1833 g         2011 t
ORIGIN      
        1 aggatgtccg gattacaacc gggggttcca tcggccagat ccggatgcaa ctttcgcacc
       61 ggtatgctca ggtggatgaa ctcgtagatc cattcagagg tatccagttc actttcacca
      121 tctgcaatcc aggtgatgtc ttcctcttca tcctcctctt caggctgctc accctttttt
      181 acatagaggg tgtgattgcc gtggatcgga agatcaagtg cttgcccgca acgatcgcac
      241 tctgtgctga tggttccgga aatatcgaaa ttcagaacga aaagtctttc ctttttatcg
      301 aaggcaagat ccacaaacag cccgcattca cgaatcagac tgtctgggaa atgggaaaaa
      361 aaaggtgcgc cgatctcaaa gttgaaatgg tgcaacccgt tctttagccc gataaagggt
      421 ataacatatt ccctcagacc cttcatcctt caataaaagc ccacaaagtt agctataatt
      481 caggaaatct gaaatttatc cccgctcctg ggctaattct gttcgtttca ccggattttc
      541 atgattctga acatactgca gccgtgttct gtatatatca agggcacaga atattgactg
      601 aagcatagag gtagcagagg caacattctt tcctgctata tcgtaggctg tgccatggtc
      661 aggcgaggtg cgcacaaatg gcattcctgc cgtataattg attcctgtgt caaatgaaag
      721 tgctttaaaa ccaacaagcc cctggtcatg atacatggta aggacagcat caaactgact
      781 gatttgtccg cttccgaaaa gaccatcggc agcaaaagga cctgaaacga tgataccatc
      841 cttccgcacc tgttccatag caggtatgat cacctccagt tcatctctgc ccaacaaacc
      901 ctggtctccg gcatgcggat tcaatccggt cagtgcaatc aatggtttgt caatcccgaa
      961 atcctgtcgc aaactggtgg ccagaatgcg tatcttggat accaggtatt ctacactcaa
     1021 agttccggga acttcctgca aaggcatatg atgggttgcc agagcaactc tcatggaatc
     1081 atttaacaac atcatcagac taccgggtcc acctgcacgg tgattcaggt attcagtgtg
     1141 gccaggaaat gtaaacccgg cttcatgcat attgtgctta ttgatgggtg cagtaaccaa
     1201 tgcgtctaac tgaccgtcta acaaggcatc tgccgccgct tccagtgata acatggcagc
     1261 ctgcccgcca gcagtactgg aaatgcccgg atctggaatg aactcctccg gaagtgtgtt
     1321 gatcacattg atcagtttcg gatttgcatc agagacctga tcacagtggg catatttaaa
     1381 atgttctgca tcaagcagct tcttataaaa ggacagcact ttgtttgagc caaaaatgac
     1441 aggcgtacag taattcagga tcctgtgatc gctgagcgcc ttgatcagta cctccggccc
     1501 gatgccattg atatctccga ttgtaaagcc aattcgtact tttttttcca tacctgaagt
     1561 caataccttt gatcggcaca aaagtaagca gtcctcacgg aatcaacccg catgaatcca
     1621 gcacacagtt tcaggcataa aaaatcactg ggacagcatt ttctcaggga tccggatatc
     1681 gcagaaagga ttctggatgc cataccggca gaagccgctg atgcagccgt gctggagatc
     1741 ggtgccggtg caggggtact taccaggcca ttgttcaggg cattcggccg gaagttgtat
     1801 gtcagtgaga tcgaccatcg atcggtacgc ttgattgcaa ctgaccccgg tgtgtctgca
     1861 gatcggatcc ttgaaggaga tttcctgaag tgtaatctgc aggagcttcc cacaccttta
     1921 tatattgcag gtaacttccc ctataatatc agctctcaga tcgttttcag gatgttagag
     1981 cacagagaaa agattcccgc cttggtgggg atgttccagc gggaaatggc tgtgcgtatt
     2041 tgtgcatcac cgggaaaccg cgattatggg gtcatttctg tctggacgaa tttgttctac
     2101 tccggagaga tcctgtttga attgccaccg gaagcatttg atccgccacc taaagtgcat
     2161 tcggcggtca tcaggcttat aaaaagagct gaagttgtgc ccggtgctga cgatgccgga
     2221 gtgctgaaac tggtcaaaat ggcttttcag caaaggagaa aaaaactgag taatgccctt
     2281 ggtgctgtcg atggagctag gcaggctctg gaagggtttg gctggcaaga cctcagacca
     2341 gaagcactgg aacctgtgca gtatgtacag ttgtatcagg caattgtccg gtcatgaaaa
     2401 tactgataac cgacctcgtt caccctgatt tggtcagctt gcttgctgaa cgcgggcatc
     2461 aggtcacttc tgatcctgcc atctcctaca agcggttatc agaagtgatc gcaccgtttg
     2521 agggccttat cgtcagtacc aggctaccca ttgacaaagc gatcattgat gcagctccga
     2581 atctacggtt tattgccaga gtagggtctg gtatggaaca catagacatg ctctatgcgc
     2641 aggagaaggg aattagggtg gtcagttcgc ctgaaggaaa tgccaatgca gttgctgaac
     2701 atgctcttgg tatgctcctg gcactattgc gtcatatacc cagggcggtt caggaaattc
     2761 ggcagcaaaa atggatccgg gaggcaaaca ggggtataga actgaaaggc agaaccattg
     2821 ggattattgg tgttggacat acaggaacag cctttgcacg gaaactcagc ggaatggaaa
     2881 tgcgggttct ggggtatgat aaatacagga atagtactga cctgttgtat gttacacctg
     2941 cctccatcca ggaaatttcg gaacaggctg agattatcag ctttcacgtt ccgcttactg
     3001 ctgaaacgca ctattatctg aacaggagtt tcattgataa catgaaaaat ccgttcattc
     3061 tgatcaatac cagcaggggt aaagtggtca gtacggttga cctgattgat gggttgtctt
     3121 caggaaagat cagaggcgca gcacttgatg tactggaaaa tgagcagttc agcctgtaca
     3181 caaccaaaga aaaagaggtt catgaagcct tgatggcttt tgaagaccgg gtgctcataa
     3241 cacctcatat agctgggtgg acaaaagagt caaaatacct gttatcttcg gtgcttctgg
     3301 ataaactcag cgatctcctg taaaaaggat tacattagcc ttaacttatt tttacggccg
     3361 ggtgacctga aaaattatac aggaccctat ttttgttttc ggatatttag atatttttac
     3421 gccttattaa caatttttaa aacttggtag tatgaggaaa ttcttctacg ctctggtaat
     3481 cctgatagta tccgctggtg ctgctatggc acagaaaggg gagatacagg gaaaggtggt
     3541 agatgagaaa ggtgaaggca tcccctttgc caacgtcgct gtgtttaagg atggagtgct
     3601 ggaaaccggg gctcaaaccg actttgacgg attgtattcc gtgtccggtc tggtgccagg
     3661 tacctatgat gtggaagcat cttaccaggg aaacaaattt cgtatcaccg atatcatcgt
     3721 ttcatccggt atcatcttcc tggatgagat tacccttaaa gaaggggttt cgctcgatga
     3781 ggtggtcatt gtgtacgaag ctccccttgt tgataaagga aatacatcta caggtggtgt
     3841 ggtgaccaag gaagacattc agcgcatagc taccaggaat gtgaccagta ttgctgcaac
     3901 caaggaaggg gtgtatcaga gtgacgaagg tggcggactg aacatcaaag gctcccgtgg
     3961 agatgctact gaatatatag tagatggtgt aagggtttcc ggttcactaa agctcccgca
     4021 ggatgccatt gaacagctgg aggtcatcac cggtggtgtg gatcccaagt atggtgatgc
     4081 aaccgggggg tttgtaacca tcactacccg cggtccaagt aaagactata acggtagcgt
     4141 agaactggct tccagccagt ttctcgatcc ttacggatac aatcttgcca cattattcct
     4201 cactggccct attatcacca aaaacaaggg caccgattct gccagtgcca aactcggttt
     4261 cttcattgct gcagaatatg aaggagaaaa agatagcgat ccgtcagctt tcggcaattt
     4321 caagctgaag gatgacgtgg cagcttcctt gctggataac ccgcttcgcc cggcagtcac
     4381 tggtacgggt ttcaataaga atacagagtt cctgaccaac gatgactttg aagttgtaga
     4441 tgcaagacag aatgtggcca gccgcggcct tgcacttacc tcacgaatag actataagtt
     4501 gtccaaaaca atgaacttcc aggtcggttt gacttaccgg aatttctttg ctcacaacta
     4561 tgtgagaacc tttgcactcc tgaacccgga aaacaatccg gtctccaatc agaatacata
     4621 ccgtggattt gtacgtttca cccagcgatt cccggaaagg aaggctgctg aaggagaatc
     4681 ttcctctatt ctcggaaacg cttattacaa tatccagttt gactatacca agtattacgg
     4741 taccagcatg gatgaggatc atgaaatgaa tcccttcaac tatggctata taggagcatt
     4801 tgatacttat cgctctccgg tatatttcta tggaacagat gaagtgtcag gtcttaacgg
     4861 atggtatctg gccggctatc aggacacact ggtaactttc gatgcaggta ctctgaaccc
     4921 tgaactggct acttataccc aaacttatta cgacctggcc tctgcgcctc ccggctcgtt
     4981 gtttgatatc cagctgggta gtggtctgct gaacggcgat gcttttgtga actccacttc
     5041 cacctacagc ttgtggtgga gccgtggtgt tcccttcagt tcttacaaca agcagaacga
     5101 cgatcagtat gggcttaatt tcaacgcagc tcttgacctc aagagtaaca agaagtctgc
     5161 caacatctct aagcacagta tagagtttgg tttcgaattc cagcagcgta ttgaacgctt
     5221 ctatcaggta gcgcctattg gactctggac acttgcccgc cagctggcca accgtcacat
     5281 ccagaccctg gatacagata atccgattct ggttattgat ggccagcaat attcttatga
     5341 tgaatacctg gcatcaggaa tcgagttcgg tgcctatgat acgatttttt acaacaggtt
     5401 attcattgca gaagaccagt cattctttga tgaacaactg cgggataaac tgaatgagct
     5461 tggctatggt gttgaacagc ttgacttcat taacgtggat gcactgcctc tggacgtgct
     5521 gtcgctcgac ctgttcagcg ctgatgaact ccttaataac ggtaacgcgt ttgtattcta
     5581 caatggttac gattatttgg gtaatcaatt gaccacacaa ccttcctttg gagatttctt
     5641 tacagaaaaa gatgaattcg gaaactacac cagacccatt gcagctttcc gtccggttta
     5701 tactgccggc tatattcaag acagattcaa ctttaacgat atcgtattcc gggtaggtgt
     5761 tcgtgtggat cggtatgatg ccaaccgcag cgtacttcag gataagtact ccttatatga
     5821 tatctactcc aaaggtgaaa ttgacggaag ccagaacccc aatggagccc acccgggtaa
     5881 tattggagat gattatgcag tgtatgttga tgattataac agcccttctc cggtaatctt
     5941 aggataccgt gatggagata cctggttcaa tgcagatggt gaagaggttt ctgatccaag
     6001 aacaattgcc ctgagctcca gctcaggtgt gatcacccct tatctggttg atccggatgc
     6061 caatatcaaa gacgagaact ttgaccccag ctcttccttt aaggattatg tgccgcagat
     6121 atctgtgatg ccaagggtgg cattctcttt cccgatctct aaggaaacaa acagagaagc
     6181 cttgttcttt gctcattatg atatcctgac tcaacgtccg ccaacaggaa catcggcaac
     6241 cgcctatgat tactacttct tccaggagaa tgtcaataac gtattcgata accctgacct
     6301 gagacccgaa aaaacaattg attaccagat cggtttccag cagcagctga acgaagtatc
     6361 tgttctgaag atttctgcct tctatcgcga gttacgtgac atgattcaga tcatcagtgt
     6421 accatatgcc ttcccggttc agtacaccac ttacggaaat attgactttg gtaccgttaa
     6481 gggtctgaca gttacctatg atctggccaa acgtaccaag aacctgaaga tgtccacctc
     6541 ctacacactg cagtttgcag acggaacagg ttcgagctct acctcacagc tgaacctggt
     6601 aggatccggt cagcctaacc tgcgtgccat catcccactg agctatgatt ctcgccacaa
     6661 ctttaaagtg gtgctggatt accgctttga tgacccgaat ggcagccagc caagctggtt
     6721 ggatctggtt ggtctgaacc ttaccatgaa cgcccgttca ggagaacctt atactcgtca
     6781 gcagaatcct accccaactg ctcagtttgg tgtggctacc cgtgctaatc tggaaggcgc
     6841 tatcaacggt tctcgattgc catggcactt ccgtacagac atgaggcttg acaagagctt
     6901 tggtctgaca atgggaagtg gttccaaaga aagacagctg aacctgaatg cttatctctg
     6961 ggtgcagaac ctgtttgata accgaaatgt gatctctgtg tatgccttta ccggtaaccc
     7021 tgatgatgac ggctatctgt catcagcact cgggcaggag gctattgaag gccaggtgaa
     7081 tgccgagtct ttcattgacc tgtacactat gaagatgcag aaccctaata actatagcat
     7141 acctcgtcgt atcagactgg gtatcggtat tgatttctaa aattgagcaa acagtaacat
     7201 atgtggaata aaaggatcat agttgcctcg gcattctccc tgctcaccgt tatgtcggta
     7261 caggctaaag agaatatcgg tcttgttgga aaaaacgccc agagttacag ccgtctggca
     7321 tccaattgta atgatgctac ggctcagata gagctggata tcaacaacgt ccgtaccagg
     7381 atactgggag ccggcgactt ttggtgggat ctgaataacg gaaagtatgt tattccgaaa
     7441 gtttatccgc ctgaaattga agtcagttca acctttgctg gtgctatgtg gatcggcgga
     7501 attgacgccg gtggtcagtt aaagatcgca gcgcagacct accgtcagag tggtaacgac
     7561 ttctggccgg gtccgcttag tgatgctggt actacagatg atgctgtttg tacttcctat
     7621 gaccgtcact ggaaagtgaa caggagt
//
LOCUS       DAOMAX010000118         7549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1474_length_7549_cov_3.671737, whole genome shotgun sequence.
ACCESSION   DAOMAX010000118 DAOMAX010000000
VERSION     DAOMAX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_7549_cov_3.671737"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK742_07415"
     CDS             complement(<1..162)
                     /locus_tag="PK742_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU76457.1"
                     /translation="MQDEKVIHFTHAGIYQGKTPVLSDVNFEIDHGEFVYLIGKTGTG
                     KSSLLKTIYG"
     gene            complement(231..1235)
                     /locus_tag="PK742_07420"
     CDS             complement(231..1235)
                     /locus_tag="PK742_07420"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQU76458.1"
                     /translation="MYRHAFFVFVMVGITSSLFAQSDTLVIAFGSCNKTDADQSYWDL
                     VAVHHPDIWLWLGDNIYADTNDARVMDSLYQVQYNNPYYHSFIRQIQVDGTWDDHDYG
                     LNDGGKEWTFKDSSKQLFTHFMRLDDREDLRSHPGVYHATDLQVDGEIVRILALDTRS
                     FRDTLTPSPDPSKRYIPRPDGNMLGEAQWTWLQEQLADTTVSMFILLSSVQVIATEQG
                     YEKWENLQASRTRLLDMIRTSSPEGIVILSGDRHIAEYSVIGGGLDYPLLDITSSGLT
                     HTWGQIQEEANQFRMGPLMIEKNFGILQLYRDDSGLGMRSTIYSAVDGRILMTYICPF
                     "
     gene            complement(1286..2044)
                     /locus_tag="PK742_07425"
     CDS             complement(1286..2044)
                     /locus_tag="PK742_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HQU76459.1"
                     /translation="MHRLTISLLCTLLTALTPVFATNHLQADTNENQHIAIPADLSDF
                     FQAADGFMQANVKNGMVSYSALAKDHTGLDKLVSAIGSTDLTEASKDTRIAFYLNAYN
                     LLTIHQVVQNWPMASPLDKKGFFDATTFTVAGQQMTLNDIENKKLRPDPRVHFALVCG
                     ARGCPKIMNAAYMPDKVQTQLNMQTKKAMDDSSFIKVNGSKVEVSEIFKWYADDFVKH
                     SGSVEAYINKYRSSTLPSGSTIGYYTYDWKVNGQ"
     gene            2116..2517
                     /locus_tag="PK742_07430"
     CDS             2116..2517
                     /locus_tag="PK742_07430"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQU76460.1"
                     /translation="MMHNLQERIDRSVTHTVKAIFPNTTNHYDTLFGGTALAWMDEIA
                     FITATRFSRQKMVTVSSDRVDFKKPVPAGTLAELIGTVKSVGTTSLVIRVEVYLEQMY
                     ADEREKAMEGLFTMVAIDDNKHPVPISFDNN"
     gene            2576..2803
                     /locus_tag="PK742_07435"
     CDS             2576..2803
                     /locus_tag="PK742_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76461.1"
                     /translation="MKKLSLLFGVLFVAAATLSSCQKCTVCTHIELSTGEEVVEEYCG
                     SGGEVGRFEDEWKSNWDSLGGYCTRDGSTIQ"
     gene            complement(2789..3664)
                     /locus_tag="PK742_07440"
     CDS             complement(2789..3664)
                     /locus_tag="PK742_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76462.1"
                     /translation="MSVRFEAFYTAAFQTSLNDLDRIRRRIITNYFVIGFVMFAGFTF
                     EAITWMLIGILVIVLFVMFYIRTFGISTQYYQQQFFLRIQGSIGRFLLPSSSFDLNRY
                     IKLTELQAAGLIVHHPQQYGGRNMIEHAVGDDQIRIAEMDIRSSGRGEDGKPFQRTDF
                     SGIVGVHAMHTGIANPLMLVHHDLLKTEPDPSIRRKIDDHQDLVAWYVDKEAFARWFS
                     PSQLYQVVSFMEAANKQVFISVFEGGVCVAVAIPEKEKFRDAGLWTKANQEKEIRSLF
                     ETYRFVSEMLLSLNG"
     gene            complement(3677..4438)
                     /locus_tag="PK742_07445"
     CDS             complement(3677..4438)
                     /locus_tag="PK742_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQU76463.1"
                     /translation="MTLQSGAYPELELSSKNDLYLGGPRTRWQELWFAWDVFREFIKG
                     FRALHFIGPCITVFGSARFKEEHKHYQTARDTAGKLSKAGFTILTGGGPGIMEAANRG
                     AYETGGLSVGCNIVLPHEQSENPYMHRWVTMKYFFVRKVLLVKYSYGFIVMPGGMGTM
                     DELFETLTLIQTGVIRNFPVVLMGSEYYSHIMQMLERMQQEGTISPKDMYLLKVTDDP
                     DEALHHIQHYVKENYTVRRLRPRRWLGEKPLRQAS"
     gene            4517..5065
                     /locus_tag="PK742_07450"
     CDS             4517..5065
                     /locus_tag="PK742_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76464.1"
                     /translation="MKRVLLILVLFAVSEMVQAQFLDLGIKSGIQFSNDAGRLSQAEV
                     PVAEAGFNTTFGGYLQMHFNRLSVTPEFLFSSTRFTEQSAFGMDDVHLFKNDRFDLPL
                     VFGYEFFDFLRIEAGPNFYNIRDDEYEKLFRMRNLQKEFTLGAAIDMGKLTLGARMVN
                     DFGDIFQMDANTYQFTLSYDVF"
     gene            complement(5046..5939)
                     /locus_tag="PK742_07455"
     CDS             complement(5046..5939)
                     /locus_tag="PK742_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQU76465.1"
                     /translation="MKRIAFWVSYPFMLLVAALPLRALYLFSDILYVIIYHLMGYRRR
                     LVWKNLRRSFPEQDKETLRRWRKKFYHFLADTIVETVKTISIRNERLYSRFRFDNLDM
                     ISEALQQNRNIMIVLGHYGNWEWGAGAISRQLARPLMAPYAPLRNPDFNRLMVRTRSR
                     YGLQLLPRKQLIPYLLSHTQQKHFLALIADQSPNPKRQRIMQFMNQETAVVTGMESIA
                     RDFDFMVYYASTRFIKRGEYACRFELLSEHPAEMKEGDLTVAFMKKLERDIMNDPIHY
                     LWSHNRWKLTTNSKHPKKRHN"
     gene            complement(5949..6782)
                     /locus_tag="PK742_07460"
     CDS             complement(5949..6782)
                     /locus_tag="PK742_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HQU76466.1"
                     /translation="MSYPNPPRMPKNGRSVILIAGVIIALLILVFNVAVTVKAGEAGV
                     LFRPFSGGVDVNRTYDEGFHIIAPWNNMITYEVRQQEVLETMAVLSSNGLEITVDVSI
                     WYQPEYQDLPKLHKEKGQNYLDRIVKPSMRSATRSVIGRYTPEEIYSTKRDAIQKEIF
                     EETARLLDNEYVQVNAVLVRDITLPSSIKQAIENKLTQEQASLEYEFRLEREQKEAER
                     IRIQAEGKAEANKILNASLTENILKEKGIQATLELASSPNSKVVVIGNSDGLPIILND
                     K"
     gene            complement(6852..>7549)
                     /locus_tag="PK742_07465"
     CDS             complement(6852..>7549)
                     /locus_tag="PK742_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76467.1"
                     /translation="AMHEVIGHASGKINDGVGTPKETLKSYASTLEEGRADLVALYYI
                     YDQKLVDLGLMPSLELGKAEYDGYIRNGMMLQLRRLELGANVEESHMRNRQMIAQWVY
                     EKGRKEKVIEKKVKDGKTYFVINDYDKLHDLFGQLLREIQRIKSEGDYAAGKALVENY
                     GVKVNKDLHKEVLERTAKLTGAPYSGFINPKLIPVFDSNGDISDIRVEYPESFTKQMM
                     DYARRYSFLPEHN"
BASE COUNT         1847 a         1826 c         1783 g         2093 t
ORIGIN      
        1 accgtaaatg gttttgagca gactgctctt tcctgtgccg gtcttgccta tcaggtaaac
       61 gaattctccg tggtctatct caaaattgac atcactgagt accggtgtct taccctggta
      121 aattccggca tgggtaaagt gtatgacctt ttcatcctgc atgctataaa ggtatgcgtg
      181 gcagccagct attcattaca ggttatgaac aacagcaatg gtggtatgta tcaaaacggg
      241 cagatgtagg tcatcagtat gcggccatcc acagctgagt aaatggtact gcgcataccc
      301 aggccggaat catcccggta cagttgtagt atgccgaagt tcttttcgat catgagtggg
      361 cccattctaa actggttggc ctcttcctgt atctgacccc aggtatgggt cagaccgctg
      421 gaggtgatgt ccagcagcgg atagtccagt ccgccaccta tgaccgagta ctctgcaata
      481 tgccggtcgc cgctcagtat gacgatgccc tctggtgatg atgtgcgtat catatccagc
      541 aaccgggttc tggatgcctg caggttttcc catttttcat agccctgttc cgttgcgatc
      601 acctgtacac tggacaacaa aatgaacatg gaaacagtgg tatctgccag ctgttcctgc
      661 agccaggtcc attgcgcttc acccagcatg ttgccatcag gtcttggtat atacctttta
      721 gaagggtcgg gtgaaggtgt cagggtatct ctgaagctgc gggtatccaa tgccagtatg
      781 cgcacgatct ctccgtcaac ctgcagatcg gtagcatggt acacgcccgg atgcgagcga
      841 aggtcttccc ggtcatccag cctcataaag tgggtgaata actgcttgct gctgtctttg
      901 aaggtccatt cttttccgcc gtcattcagg ccgtagtcgt ggtcatccca ggtgccgtca
      961 acctgaatct gccgtatgaa actgtggtag tatgggttgt tatattgtac ctgatacaaa
     1021 ctgtccatta cacgggcatc attggtatca gcatagatat tatcgcccag ccaaagccag
     1081 atatccgggt gatgaactgc caccagatcc caataagact gatctgcatc tgtcttgtta
     1141 catgaaccaa atgcgattac cagcgtgtct gattgtgcaa acagagagct ggtaatgcca
     1201 accatcacaa aaacaaaaaa ggcatgtctg tacatgcctt aaaggtaagg ggaggggatg
     1261 aatcttctgt gaacagcaaa tgaacttact gcccgttcac cttccagtca taggtgtagt
     1321 aaccgattgt ggatcctgac ggcaatgtgg aactgcggta tttgttgata taagcctcga
     1381 ccgaaccact gtgctttaca aagtcatcgg cataccactt gaatatctct gaaacttcca
     1441 ctttagaacc gttcaccttg ataaaactgc tgtcatccat cgcctttttg gtctgcatgt
     1501 tgagttgcgt ctggaccttg tcaggcatat aggctgcgtt catgatcttc ggacaaccac
     1561 gggcaccgca aacaagggca aaatgcaccc gggggtcagg acgcagtttc ttgttttcta
     1621 tatcgttcag agtcatttgt tgtccggcta ctgtaaacgt agttgcgtca aagaaccctt
     1681 tcttgtctag cggagaagcc atcggccagt tctgtaccac ctggtggatg gtgagcaggt
     1741 tgtaggcgtt caggtaaaag gcgatcctgg tgtctttgga tgcttcggtc aggtcagtac
     1801 tgccaatggc gctgaccagc ttgtccaggc ctgtgtggtc ttttgccagt gcagaataag
     1861 agaccattcc attcttcacg tttgcctgca tgaacccatc ggctgcctga aaaaagtctg
     1921 acaggtctgc tggtatagct atatgctggt tctcattggt atctgcctgc aaatgatttg
     1981 tagcgaaaac tggagtcaat gctgtcagga gggtgcataa aagggaaata gtgagtctgt
     2041 gcatcttgta tttttgatgg aataaaggta cggacattca acagtgtctg agcctacatt
     2101 atcaataatc gagccatgat gcataacctg caggagagaa ttgacaggtc tgtgacccat
     2161 acggtcaaag cgatcttccc caataccacc aaccactatg ataccctttt tggtggcacg
     2221 gcactggcgt ggatggacga gatcgccttt atcactgcca ccaggttctc acgccagaag
     2281 atggtgaccg tttccagcga tagggtggac ttcaagaaac ccgtgccggc cggtacgctg
     2341 gcagaactga tcggtacggt taaaagtgta ggtactacca gcctggtgat ccgggtggag
     2401 gtctatctgg aacagatgta tgcagatgag cgggaaaagg ccatggaagg gttgttcacc
     2461 atggttgcga tagatgacaa caagcatccg gtgcctattt cttttgacaa taactgacca
     2521 tctggtgcgt ttcagaccca ccggaattca ctacctttaa cgcatatttt cagctatgaa
     2581 aaaactttcc ctgttatttg gagtactctt cgtggcagca gctacactca gttcctgcca
     2641 gaaatgcacg gtttgtaccc atattgagct gagtaccggc gaagaggtcg tggaagaata
     2701 ttgcggctca ggcggggaag ttggtcgttt cgaagacgaa tggaaatcca actgggattc
     2761 acttggcgga tactgcaccc gcgatggttc aaccattcag tgacagcagc atttcgctca
     2821 caaagcggta ggtttcaaac aggcttctga tctccttttc ctgattcgcc ttggtccaca
     2881 agccggcatc tctgaatttt tctttttcag gtatggcaac tgccacgcat acgccaccct
     2941 caaacacgct gatgaatacc tgtttgttgg cagcctccat aaaacttact acctgataca
     3001 gctgtgaagg agaaaaccat ctggcaaatg cctctttatc cacataccag gctaccagat
     3061 cctgatggtc atctattttc ctcctgatgg atggatcagg ctcggttttc agaagatcat
     3121 ggtgcaccag catgagcggg tttgctatgc cggtgtgcat ggcatgtaca ccaactatac
     3181 ctgaaaaatc ggttcgttga aacggcttgc cgtcttcacc tcttcccgat gaacgtatat
     3241 ccatttctgc aattctgatc tgatcatcac ctactgcgtg ctctatcata ttgcggccac
     3301 catattgctg cggatggtgg acaatcaaac ctgctgcctg caactcagta agtttgatat
     3361 acctgttcag atcaaaggat gacgagggaa gtaagaaccg gcctatactt ccctgtatgc
     3421 gcagaaagaa ttgctgctga taatactggg tggagatgcc gaatgtgcgg atgtagaaca
     3481 tcacaaatag tacgatcacg agaattccga tcagcatcca ggtaatggct tcgaaagtga
     3541 agccggcaaa catgacgaaa ccgatcacaa aataattggt gatgatgcgc cgccgaatac
     3601 gatccaggtc gttcaggctt gtctgaaaag cggccgtata gaatgcttca aaccgtacac
     3661 tcataaagaa gtagttttaa gatgcctgtc tgagtggctt ttctcccagc cacctgcggg
     3721 gacgaagacg cctgactgtg taattctctt tcacataatg ctgaatatga tggagggctt
     3781 catccggatc atcggtcacc tttagcagat acatatcttt cggcgagatg gttccttcct
     3841 gctgcatgcg ttccagcatc tgcatgatat ggctgtagta ttcgcttccc atcagtacca
     3901 caggaaagtt cctgatcacc ccggtctgga tcagtgtgag tgtttcaaac agctcatcca
     3961 tggtacccat gcctcccggc attactatga atccatagga atattttacc agcagcacct
     4021 ttcttacaaa gaaatacttc atcgtgaccc aacggtgcat gtatggattt tcactttgct
     4081 cgtggggcag aacaatattg catcctacac tgagtccgcc ggtttcatag gcaccgcgat
     4141 tggctgcttc catgattccc ggacccccac ctgtaagaat ggtgaatccc gccttgctga
     4201 gtttgcctgc ggtgtctctg gctgtctggt aatgtttgtg ttcctctttg aatctggcag
     4261 atccgaatac agtgatacaa ggaccaatga aatgcaaagc acgaaagcct ttgatgaatt
     4321 cacgaaatac atcccatgca aaccacaatt cctgccagcg ggttctgggg ccgcctaaat
     4381 acaggtcgtt cttagaggaa agttccagct ctggataggc acctgattgt aaagtcatac
     4441 cacaagtata tctgtttctt caccggccgc gttacagtat ggcacgacct ttggtttaca
     4501 agggcaaatg tacgttatga aaagagtatt gttgatcctg gtcctgtttg ccgtttcaga
     4561 aatggtgcag gcccaattcc tggacctggg aatcaaatcc ggaattcaat tcagtaacga
     4621 tgccggcaga ttgtctcagg cagaagtacc ggttgcagaa gcaggcttca ataccacttt
     4681 tggcggctac ctgcagatgc atttcaaccg gctttctgtt acacctgagt ttctgttcag
     4741 cagcaccagg ttcaccgagc aaagtgcctt tggcatggac gatgtgcacc tgttcaaaaa
     4801 tgaccggttt gatctgcccc tcgtgtttgg ctatgagttc ttcgattttc ttcgcataga
     4861 agcaggcccc aatttctata atataagaga tgatgaatat gaaaaactat ttcgtatgag
     4921 aaatcttcaa aaagaattta ccctgggtgc agctatagat atgggtaagc ttacccttgg
     4981 agccagaatg gtaaatgact tcggagatat ctttcagatg gatgccaaca cctatcagtt
     5041 cactctcagt tatgacgttt tttaggatgt ttactgttgg tggtcagctt ccatcggttg
     5101 tgactccata agtaatgaat gggatcgttc atgatatccc gctccagctt tttcatgaag
     5161 gcgactgtca ggtcgccttc tttcatttct gcgggatgct cgctcaacaa ctcaaaccgg
     5221 caggcatatt caccccgttt gataaaccgt gtgctggcat agtaaaccat gaaatcgaaa
     5281 tcacgtgcga tagactccat tccggtaact acagctgttt cctgattcat gaattgcatg
     5341 atgcgctggc gtttcgggtt ggggctctga tctgctataa gcgccagaaa atgtttttgt
     5401 tgtgtgtgcg agaggagata aggtatcagc tgttttcttg gaagcaactg caaaccatat
     5461 cgagatctgg tgcgcaccat caggcggttg aagtcaggat tgcgcagcgg ggcatagggt
     5521 gccatgagcg gcctggccag ttgtctgctg atagcaccag cgccccattc ccagttgcca
     5581 taatgcccca gcacgatcat gatgttcctg ttctgttgca gtgcctctga tatcatatcc
     5641 agattgtcaa acctgaaacg gctgtaaagc ctctcattac gaatagaaat ggtcttgacc
     5701 gtttctacta tggtatctgc aaggaagtga tagaatttct ttcgccacct gcgcagggtc
     5761 tctttgtcct gttccggaaa agaccgtcgc aggttcttcc agaccaggcg ccttctgtag
     5821 cccatcagat gatatatgat cacatataaa atatcactga acaggtacag ggctctcaat
     5881 ggcagcgcag ctaccagcag catgaaaggg taggagaccc agaatgcaat acgtttcatc
     5941 ctcttttctt acttgtcatt gaggatgatg ggcaacccgt cactgttacc tatcactact
     6001 actttgctgt taggtgagga ggctaattcc agggtagcct gaatgccttt ttcctttaga
     6061 atgttctcgg tcagtgaggc attcaggatc ttgttggcct ctgcctttcc ttccgcctga
     6121 atacggatac gttctgcctc cttctgttct ctttccagtc tgaactcata ttccagcgaa
     6181 gcctgctcct gggtgagttt gttctcgatc gcctgcttga tggaagaagg caacgttatg
     6241 tcacgcacaa gaactgcatt tacctgaaca tattcattgt ccagtaaacg ggctgtttct
     6301 tcaaatatct ctttttgaat ggcatctctt ttggtgctgt agatctcttc cggtgtatat
     6361 ctgccgatca cacttcgggt ggctgaacgc atagatggct tgactatcct gtccagataa
     6421 ttctggcctt tttccttatg cagcttcgga agatcctggt attccggctg gtaccagata
     6481 gaaacatcaa ccgtgatctc cagaccattt gaagacaata ccgccatggt ttccagcact
     6541 tcctgctgtc tgacctcata ggtgatcata ttgttccagg gggcgatgat gtgaaagcct
     6601 tcatcgtagg tgcggttcac atctacacca cctgaaaagg ggcggaatag cacaccggct
     6661 tcacctgcct ttacagtaac ggctacattg aagacaagaa tgagtaatgc gatgataaca
     6721 cctgctatga gtataacgct gcggccattc tttggcatgc ggggaggatt aggataagac
     6781 ataaagtata aaattcgtta aacaaaaaag ggccggatcg ttccggccct gtaaaggtag
     6841 ttttttgatc ctcaattatg ttccggcagg aagctgtaac gacgggcata gtccatcatt
     6901 tgcttcgtga aactttccgg atattccaca cgaatatcag agatatcgcc attggaatca
     6961 aatacaggta tcagtttagg attgatgaac ccactgtaag gagcaccggt cagcttggca
     7021 gtgcgctcca gtacttcttt gtggaggtct ttgttcactt tcactccata gttctctacc
     7081 agtgctttac cggcggcata atcaccctct gatttgatgc gctggatttc tcgaagcaac
     7141 tggccaaaca ggtcatgcag tttgtcataa tcgttgatga caaaataagt tttgccatct
     7201 ttcactttct tttcaatgac cttttctttc ctgcctttct catataccca ctgggcgatc
     7261 atctgcctgt ttcgcatatg tgattcttct acattggcac ccagttcgag cctccgcagc
     7321 tgaagcatca ttccattgcg gatatagccg tcatattcag cctttcccaa ttccagactg
     7381 ggcatcaacc caaggtcaac cagcttctgg tcatagatat aataaagtgc taccaggtct
     7441 gcacgtcctt cttccagggt gctggcataa ctcttcaacg tttctttcgg ggtgcccaca
     7501 ccatcgttga tcttacctga tgcatgtcct atcacttcat gcatggcgg
//
LOCUS       DAOMAX010000119         7544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1476_length_7544_cov_4.779944, whole genome shotgun sequence.
ACCESSION   DAOMAX010000119 DAOMAX010000000
VERSION     DAOMAX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_7544_cov_4.779944"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK742_07470"
     CDS             <1..214
                     /locus_tag="PK742_07470"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76468.1"
                     /translation="ALDQLIVSESILTDDAAFRVDDRKVRFIRNDFQMYHSDKYGDTP
                     NRTYGGNNYYGGYSDHLPAYFELILR"
     gene            complement(604..1440)
                     /locus_tag="PK742_07475"
     CDS             complement(604..1440)
                     /locus_tag="PK742_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76469.1"
                     /translation="MNNIKKYPSKIMLFGEYGVLYGGEGLAIPCNRYHAWWSKSAEDV
                     EYKLPVSLLEQMAAFCADHILISEHLDIGSWVEVMHSGETIATDIPIGYGLGSSGSVV
                     AAVYDRFHIKEVQDIKQLREILARMEDFFHQKSSGLDPLVCYLDQAVRLHGEEVELVD
                     APVTLHFELFDTEINRSTHHLVEIFRERMKDEEYSHFILDIYLPLNRSCIDAWLSNDC
                     ETLLSKLKKLSSLQLEYFNFAIPTHVWYDWGQDLAHGNHIMKLCGAGGGGYMLRFDTA
                     GI"
     gene            complement(1437..2465)
                     /locus_tag="PK742_07480"
     CDS             complement(1437..2465)
                     /locus_tag="PK742_07480"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76470.1"
                     /translation="MNVTWKSPSNIALIKYWGKYGRQLPRNASLSITLDEAHTITSVT
                     TEKKRTKKASCSLLFEGKPNPVFEAKVQDFLRSIQKDFPFLGSQHLLIETSNSFPHSA
                     GIASSASAMSALALCLCSLDILHGGKLPEDFFRKASNYARLGSGSAARSVYPEFSVWG
                     KTNLVPLSSNQYALASPVHYHANFNDIQDTILLVSRSEKSVSSRAGHALMDKHPMARI
                     RYRNAVVRLEKLLSVLTTGDWSAFASIVEAEALELHALMMTSDPSYILMTPNTLDIIQ
                     RIRDFRERTQLPVCFTLDAGPNVHVLYPYYEKRKVRNFIQKELLPLCQDNQALYDHMG
                     RGPIQIFE"
     gene            complement(2631..3167)
                     /locus_tag="PK742_07485"
     CDS             complement(2631..3167)
                     /locus_tag="PK742_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76471.1"
                     /translation="MLKKRVKAGSVTHLTDARYFAAWDVEFIGFCFDPESSDYISPQD
                     ALAIKGWLSGVQFVAEFAGQDEENIRNIISYLEPDVIELDATYPISLAKSLHNEGHTI
                     AWRGSWHDQPAETDELAYLLVTEIPSGASLSPKHMTDLSQQPDIDHDLVQLKGSPEAE
                     TGIKNYDDVGALLESWEI"
     gene            3188..3547
                     /locus_tag="PK742_07490"
     CDS             3188..3547
                     /locus_tag="PK742_07490"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQU76472.1"
                     /translation="MAKVNPSTRTKEDFFNLVWQVVRLIPKGRVTSYGAIAKFLGTGS
                     SARTVGWAMNASHHATPAVPAHRVVNRLGMLSGKHHFSTPEAMENALKREGIEVKDDK
                     VVDFAKLFWDPGKELDW"
     gene            complement(3690..4373)
                     /gene="trmB"
                     /locus_tag="PK742_07495"
     CDS             complement(3690..4373)
                     /gene="trmB"
                     /locus_tag="PK742_07495"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQU76473.1"
                     /translation="MAKRKKEKFAALNTFPNVLQNRSFTSPKAENFLGEYVDIKGHWQ
                     EQVFHNTNPITLELACGKGEYTNAMAERFPNRNFIGIDIKGNRLHNGAKMALDAGRKN
                     AAFLRTEIFLLSNFFDAGEVNEIWITFPDPHLRPSKDQQRLTSERFISIYRKIMPAGG
                     LVHLKTDDPTLYQFTLNEAPKANAELIYFNDDIYSGPLYDPLLEVKTYYEGMHLENGR
                     KIRYVRIRI"
     gene            complement(4471..5703)
                     /locus_tag="PK742_07500"
     CDS             complement(4471..5703)
                     /locus_tag="PK742_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76474.1"
                     /translation="MKNTLLLFLALCSSSLLAQVCNPGGNVLIYTNYDGGDITIDIDE
                     DIPDIRIGICSYESISIEITGDFVDNVTQVLYAGYDDDGTTSISGVPSGITDILLYPP
                     ATLSDPDGYPYIICAYDCDTDYVPGGCNTVEQVTDYFLTELGGELRYSFMQYGVWAGG
                     DYYMSEGGNCCYGADVACFTDIIAGKDQIICAGETTLMIASGADTYTWTPMDGLDCTG
                     DCAEVNASPETTTTYVVFGTDADGCFGYDTITVYVNETPDAGITVSNDTAFASGGVSY
                     QWLLDGVILPGETGTFVVAPETGNYSVIVYSSQGCADTSAMATVIVEQPQEIKLLTEQ
                     ITIYPNPADDLLQISWPSALVVEDISLFNALGQMVRMEITTESTSVTCLISQLPPGIY
                     QLQISTGSGILKQRVIIQ"
     gene            complement(5738..6817)
                     /locus_tag="PK742_07505"
     CDS             complement(5738..6817)
                     /locus_tag="PK742_07505"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HQU76475.1"
                     /translation="MRIASSLSLAPLILLTTLNACKGQQKQAEEDGEKEYKYYADWAI
                     DQEYLERGCNGGYDCIRSIDKPDFISIKEVDFIGDDDLVVGLKIGDEVRCYPHAILDW
                     HEIVNDHIGKTDFAINYCPLTGSAMAWDRHVDGMLTDFGVSGWLFNSNVIPYDRETGS
                     HWSQMKQECVNGQFYKQKATSYPVLETSWVTWKKLYPNSKILSTNTGFSRHYGEYPYD
                     TYRENEDVFFPTEHDPDSLHPKMRVYGVIDGDDAVCYRYDHFSHGPAIFQDTVFGIPV
                     IVVGSDPDNFMAVFADERNGKPLQARVIKDALPAIFEDASGNRYDLFGYCISGPDAGS
                     RLEVLNGFAAYWFAWVAFYPQTTLR"
     gene            6855..7439
                     /locus_tag="PK742_07510"
     CDS             6855..7439
                     /locus_tag="PK742_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQU76476.1"
                     /translation="MDEKRILEELIRSRRSVKPAQFGPGKVADEVVEAILESARWAPT
                     HGMTQPWRFVVFTGDGIERFTTFREEEYRRKNPGEQFNQLKFDKMRAASEKCSHIILI
                     GMKRQESRKLPEWEEVAAVSSALQNMQLCATAYGVACYWSTGAMSTDPSMLSWLELGP
                     DDLALGMLMLGQRGEEVAAPERHPQENFVRWQRK"
BASE COUNT         1890 a         1821 c         1891 g         1942 t
ORIGIN      
        1 ggcgcttgac cagcttatag tgagtgaatc catattaact gacgatgccg cttttcgggt
       61 agatgaccgt aaggttcgtt tcattcgcaa cgactttcag atgtaccaca gtgataagta
      121 tggcgatacg cccaatcgca cctatggtgg taacaactac tacggcggtt acagcgacca
      181 cctgccggcc tattttgaac ttatactgcg gtaggattca gccgtatcat aattggtttc
      241 cctaccccaa cgcaggcatg tgcagaaaca accatgctca ggtaacaaag gaacggtgcc
      301 tcctccggca gtgcgagggt agaagcatac aaatgggcag ggttttgcgt tcctgatcgc
      361 caacgctaaa gtgctgtctc tgcaaggtgt tttccagagt acaccatgtc tttgatgttg
      421 ttgcgatgaa ggaatgctca gcgccgcaaa agcaccttcg gagggggcag gcctgcctac
      481 cggtaggcag gcgttttggt gatacctttt gcgcttcaac aggtatcgaa actaaattga
      541 aggagatctg aatctgatcg ataccaaagt gttctttcga aggtcgatca ttgaaaaaaa
      601 gctctatatg ccggctgtat caaaccgcag catataacca ccaccacctg cgccgcaaag
      661 tttcatgatg tggttgccat gtgccaggtc ttgtccccag tcataccaga catgtgttgg
      721 aatggcaaaa ttgaaatact ccagctgcag cgatgacagt ttcttcagct tgctgagcag
      781 ggtctcgcag tcattggata accaggcatc gatacaggat ctgttcagag gtaaatagat
      841 atccaggatg aaatgactgt attcttcatc tttcatccgc tcgcggaata tctccaccag
      901 atggtgggtg ctgcgattga tctctgtatc aaataattca aagtgtaagg tgaccggtgc
      961 atccaccagc tccacttctt ctccatgtaa acgcacggct tgatccaggt aacaaacaag
     1021 tggatccagg ccggaactct tctggtgaaa gaaatcttcc atacgtgcca ggatctctct
     1081 gagttgtttt atgtcttgaa cctcctttat gtgaaagcga tcgtacacgg cagccactac
     1141 agatccggaa gagcccagtc cataaccgat gggaatatcc gtagcaatgg tctctccact
     1201 gtgcatcacc tccacccagc tgccgatgtc caggtgttcg ctgatcaata tatggtctgc
     1261 gcaaaaggca gccatctgtt caagcaggga aacaggcaat ttgtattcta catcttctgc
     1321 agacttgctc caccaggcat gatatcgatt gcaggggata gccagacctt caccaccata
     1381 caatacacca tactcgccaa agagcatgat cttggacggg tattttttaa tattattcat
     1441 tcaaatatct ggattggccc gcgacccatg tgatcataca acgcctggtt atcctggcaa
     1501 agtggcagca attccttctg gatgaaattg cgcactttcc ttttttcgta ataaggatac
     1561 aacacatgca cattcggtcc ggcgtccagg gtaaaacaga caggaagctg ggtgcgttcg
     1621 cggaaatcac ggatgcgctg aatgatgtcc agggtgttag gtgtcatcag gatgtatgac
     1681 ggatcactgg tcatcataag cgcatgcagt tccagtgctt ctgcttctac gatggaagcg
     1741 aatgcagacc agtcgcccgt ggtcagaacg gataacaact tctccagccg cacgaccgca
     1801 ttgcgatagc gtattctggc catcgggtgc ttgtccatca gagcatgacc ggcccggctg
     1861 gaaacagatt tttctgaacg actcaccagc aggatggtat cctggatatc attgaagttg
     1921 gcgtggtaat gtacaggact ggccagtgca tactgattgg agctgagtgg aaccaggttg
     1981 gtctttcccc agacactgaa ttcaggatag acggatcggg ctgcactgcc agaacccagc
     2041 cttgcatagt tggatgcttt gcggaaaaaa tcttccggca gcttgccgcc atgcaggata
     2101 tccagactgc agaggcataa tgccagtgca ctcatggccg atgcactcga agcgataccg
     2161 gcagaatgcg gaaagctgtt gctcgtttca atgagcagat gctgactgcc tagaaatgga
     2221 aagtcctttt ggatggaacg cagaaagtcc tgcaccttgg cctcaaagac tggattcggc
     2281 ttgccttcaa acagcagaga acaactggct tttttagtgc gctttttctc ggtggtgaca
     2341 gaggtgatgg tatgcgcctc gtctaacgtg atgctcaggc tggcattgcg tggcagctga
     2401 cggccgtact tcccccagta tttgatgagg gcgatgtttg atgggctctt ccaggtaacg
     2461 ttcacactaa gaatttcaag gcaaatctat ggaaatgtgc acttagcttc cacctgcgtt
     2521 ctgtcaggtg ttgtatcaga gaatacatgg ccatatgaac agcggcacca accagcatgg
     2581 taggccggaa acagagggtc aggtttacag ccacttgagg ctgctgctgg ctagatctcc
     2641 caggattcca gcagggcacc tacatcatcg taattcttga taccggtttc ggcttcaggt
     2701 gaacctttga gttgaaccaa gtcatggtcg atatcaggtt gctgtgacag atcggtcata
     2761 tgttttggag atagtgatgc accagatgga atttcagtta ccaggagata agccaattca
     2821 tctgtttcag caggttgatc atgccagctt ccccgccagg cgatggtatg tccttcatta
     2881 tgcagtgact ttgcaaggga aattggataa gtagcatcca gttcgatcac atcgggctcg
     2941 agatagctga tgatgttgcg gatgttctcc tcatcctgtc cggcaaattc ggcaacgaac
     3001 tgcacgccac tcaaccaccc tttgatggca agggcatcct ggggagagat gtaatcactg
     3061 gattcaggat caaaacaaaa tccgatgaat tccacatccc aggcggcaaa gtaacgggca
     3121 tcggtgagat gggtaacaga accggccttg actcttttct tcagcatcat acacgtacct
     3181 tagtgtcatg gcaaaagtaa acccttccac gcggacgaag gaagactttt tcaacctggt
     3241 atggcaggtg gttcggctca ttccgaaagg tcgtgtgacc agttatggcg ccattgccaa
     3301 atttcttgga acagggtcct ctgcccgcac ggtgggctgg gctatgaatg catctcacca
     3361 tgctactccg gcggtacctg cacaccgggt ggtcaatcgt cttggtatgc tgtcaggcaa
     3421 gcaccatttc tctacccccg aagccatgga gaatgcactc aaaagagaag gcatagaagt
     3481 gaaggacgac aaggtggttg attttgccaa actattctgg gatccgggga aagagctgga
     3541 ttggtaataa gcagtcagca gtgaaatttg gataaacaag tcagagctta tcctttttac
     3601 catttcccct ccagatggct atcgggaact atcggggttc gccttacaac catcgcttac
     3661 attccgcatt tcgccctccg gattccgcct taaattctga tcctcacata cctgatcttc
     3721 ctgccgtttt ccaggtgcat gccctcataa taggtcttca cttccagtaa cggatcatac
     3781 aatgggccac tgtaaatgtc gtcgttgaag tagatcagtt cagcatttgc ttttggtgct
     3841 tcgttgagtg tgaattgata caaagtcgga tcgtctgttt tcagatggac gagtccgccg
     3901 gctggcatga tctttctgta gatgctgatg aaccgttcgc tggttagtcg ctgctggtcc
     3961 ttactgggcc gcaggtgtgg gtcggggaaa gtgatccaga tctcatttac ttccccggca
     4021 tcgaaaaaat ttgacagaag aaagatctcc gtgcgcagaa aagcggcatt ttttctgccg
     4081 gcatccaggg ccattttagc tccgttatgc agcctgttac ccttgatatc aatgccaata
     4141 aaattcctgt ttggaaatct ttcggccatg gcatttgtgt attccccttt tccacaggcc
     4201 agttccaggg taatggggtt cgtgttgtga aaaacctgct cctgccaatg tccttttata
     4261 tccacatatt cacccaggaa attttcagct ttcggcgagg tgaatgagcg gttttgaagc
     4321 acattgggaa aggtgttcag tgctgcgaat ttttctttct tcctcttggc catcaggaca
     4381 aaaataccaa cagaagtgaa acacttccca aatacagaat atataaagta gggctcagct
     4441 ccagccggaa cgttgcagaa tactaagctg ttactgaatg atcacacgct gtttcagtat
     4501 tcctgatccg gtactgatct gcaactgata aatacccgga gggagttgac tgatcagaca
     4561 ggtcacagat gtgctttctg tagtgatctc cattcttacc atttggccta acgcattgaa
     4621 cagactgata tcttctacta ccaaagcaga tggccagctg atctgcaaca ggtcatcagc
     4681 aggattggga tatatggtga tctgctctgt aagcagtttg atttcctgag gctgttctac
     4741 gattacggta gccatggcag aggtatctgc acaaccttga gaagagtaaa cgatcacaga
     4801 ataatttcct gtttcagggg ctaccacaaa tgttcctgtt tctccgggta agatcacacc
     4861 atccagcaac cactgatagc ttactccacc ggatgcaaat gctgtatcat tggaaacggt
     4921 gatgcctgcg tcgggtgttt cattcacata gactgtgatg gtatcgtagc cgaagcagcc
     4981 atcggcatca gtaccaaaaa cgacataggt agtggtggtt tcgggactgg cgttcacctc
     5041 ggcacaatcg cccgtgcagt ccagaccgtc catcggggtc catgtatagg tatctgctcc
     5101 gctggctatc atcagggtgg tttcgccggc acagatgatc tggtcttttc ctgcaataat
     5161 atctgtgaag caagccacat cagcaccata gcaacagtta cctccttcac tcatataata
     5221 atcgccgcct gcccaaacgc catattgcat aaaggaatag cgcagttcgc cgcccagttc
     5281 tgtaaggaaa taatcggtca cctgttctac ggtgttgcat cctcccggca cataatcggt
     5341 atcgcaatcg taggcacaga tgatgtatgg gtagccatcg ggatcgctta gagtagcggg
     5401 gggatacagc aggatatctg tgataccgga tggtactccg ctgatggatg ttgtaccatc
     5461 gtcatcgtac ccggcataca aaacctgggt tacattatcc acaaaatcac ccgtgatctc
     5521 aatactgata gattcatacg agcatatacc tatacgaata tcaggaatgt cttcgtcaat
     5581 atcgatggta atgtcgccac catcataatt ggtatagatc agcacgttgc caccgggatt
     5641 acacacctga gcaagcagag aactgctgca gagagccagg aagaggagca gggtattttt
     5701 cattttggga aggttttcgc ttcccaaatt acaaaactca gcgcaatgta gtttgtggat
     5761 agaaggccac ccaggcaaac caataggcag caaagccatt taatacctcc agtctgctgc
     5821 ctgcatccgg gccggaaata caatagccga acaggtcata tctgttacct gatgcatctt
     5881 cgaaaatagc aggcaatgca tctttgatga ccctggcctg cagcggtttt ccgttccgtt
     5941 catctgcaaa cacagccatg aagttatcgg ggtcgctgcc cactactatg acagggattc
     6001 caaagaccgt gtcttggaag atggcaggtc catgactgaa atgatcatag cggtagcaga
     6061 ctgcatcatc gccatcaatg acaccataga cccgcatttt tggatgcagg ctgtcagggt
     6121 cgtgttccgt gggaaaaaat acatcttcat tttccctata ggtgtcatac gggtattcac
     6181 cgtagtgcct gctgaaaccg gtattggtac tcagtatctt gctgttgggg tagagtttct
     6241 tccacgtaac ccaggatgtt tccagcaccg gataggaagt agctttctgt ttatagaact
     6301 gaccgttcac acattcctgt ttcatctggc tccagtgtga accggtttcc cgatcgtagg
     6361 gaatgacgtt gctgttgaac aaccagccgg acacaccgaa gtcggtcagc atgccatcca
     6421 catgtcggtc ccatgccatg gctgagccgg ttagcggaca ataatttatg gcgaagtcgg
     6481 tcttgccgat atggtcgttc acgatctcat gccagtcgag gatggcatgc ggataacagc
     6541 gtacttcatc gcctatcttc aatccgacca ccaggtcatc atcaccaatg aaatctacct
     6601 ctttgatgga aatgaaatcc ggtttgtcta tcgaccggat acaatcatat ccgccattgc
     6661 aacctctttc caggtattcc tgatcgattg cccagtctgc gtagtattta tattcctttt
     6721 cgccgtcttc ttcggcttgt ttttgctgtc ctttgcaggc gttgagtgtg gtcagcagga
     6781 tgaggggagc cagacttaag gaggaagcga tgcgcacgtt gtagttttgg gcaaaattac
     6841 cacattaaga gcccatggat gaaaagagga tactggaaga attgatacgc agccggcgat
     6901 ccgtcaaacc ggcacagttc ggacctggaa aagtggcaga tgaagtagtg gaagctattt
     6961 tagaatctgc acgatgggca cctactcacg gcatgactca gccctggcgg ttcgtggtct
     7021 ttacgggtga tggtatagag cgtttcacca cttttcggga agaagaatac cggagaaaaa
     7081 atcccggtga gcaattcaat cagttgaagt tcgacaagat gcgtgctgcg tctgaaaagt
     7141 gttcgcacat cattctgata ggcatgaaac ggcaggagag tcgcaagctt cccgaatggg
     7201 aagaagtggc tgcggtcagt tcagctttgc agaatatgca gctttgtgct actgcttatg
     7261 gggtggcctg ctactggagt accggagcca tgagcaccga tccgtccatg ctgtcatggc
     7321 tggaactggg gcctgatgat ctggcactgg gcatgcttat gctgggacag cggggagagg
     7381 aagtagctgc tccggagcgt catcctcagg aaaacttcgt gcgctggcag cgtaaataag
     7441 cctgaatgat taactttgtt gccccttcgg ggacgggcct atagctcagt tggttagagc
     7501 acctgactca taatcaggtg gtccctggtt cgagcccagg tggg
//
LOCUS       DAOMAX010000120         7511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1486_length_7511_cov_4.613332, whole genome shotgun sequence.
ACCESSION   DAOMAX010000120 DAOMAX010000000
VERSION     DAOMAX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1486_length_7511_cov_4.613332"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK742_07515"
     CDS             <1..263
                     /locus_tag="PK742_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQU76477.1"
                     /translation="IAAPTAAPVKAISDFLGLDVHDIGNHYEPVPVGAVMTCEPGIYI
                     REEGIGIRIENDVMITENGLYDFMRDFPREVEEIEDIMNSRN"
     gene            269..1054
                     /locus_tag="PK742_07520"
     CDS             269..1054
                     /locus_tag="PK742_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQU76478.1"
                     /translation="MKLIRHLPNLLTLTNLFLGCMAIVYIYYDHLLIVDAKQNVYADM
                     GRMEMAAFCVFAAAIIDFFDGFVARLLGVTSSIGKQLDSLADMVTFGLVPGLIMYQLI
                     ARSYYVSAEAFDYPIMYFTSGFFLTLMAAVRLARFNTEPETAGFSGLPSPSMALFVVS
                     LPLIIFRNDAGLASMLTNKWVLLLITLLLGYLMVSRLPMPGLKLKGFAWEDNRWSYII
                     FLTGLIFGLGGILLFGLTFAVIPLVIIVYFILSLIKNISENGI"
     gene            1044..1295
                     /gene="purS"
                     /locus_tag="PK742_07525"
     CDS             1044..1295
                     /gene="purS"
                     /locus_tag="PK742_07525"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HQU76479.1"
                     /translation="MAYRAEIDIMPLKELLDPKGKAVANNMKSLQLAAVKDVRIGKHI
                     TLEIQAGSEEEARTMTETACKKLLANPIMESYTFEVFPM"
     gene            1292..1999
                     /gene="rsmI"
                     /locus_tag="PK742_07530"
     CDS             1292..1999
                     /gene="rsmI"
                     /locus_tag="PK742_07530"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQU76480.1"
                     /translation="MSKLYLVPTPIGNLADMTYRAVEVLRSADLILAEDTRTSGVLLK
                     HYGIDTKMRSYHAFNEHETVGRLASEIASGVTMALITDAGTPGISDPGYLLVQACIQQ
                     QIPIETLPGATAFVPALVNSGLPAHEFTFTGFLPPKKGRETRLKKLAEEERTLILYES
                     PHRLMKTLEQCLQFFGERPASASRELSKKFEETVRGSLTDLITHFSEHPPKGEFVLVI
                     GGKEAEKVKKRQDNPAS"
     gene            complement(1968..3368)
                     /locus_tag="PK742_07535"
     CDS             complement(1968..3368)
                     /locus_tag="PK742_07535"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQU76481.1"
                     /translation="MRTILLYAMLLLAWPASAQEFTGLRTGNFSGSNGMLLNPSSLLS
                     GNLPWDVNLVSAGAFAQNNYIYAPGQSAVGLLSAQDAETAFFTTDYVNAHSNMLVQGP
                     SGFINTGYFSFGLFTTARSAGYVHSGNFPEGVNVLENIIEDVPVQVGTVDLGLLNWME
                     FGANVGMALTRNLHVGANIKYLGGFDAITAHNREAFTFTRTDRDVELTNLLLDYSYSV
                     NAGGYPENGLYQSNLNGWGLGADLGFTWMQPVSRSRKEYAAYNWKVGVSVVDMGFIRF
                     RDGAGSYHLAANDQVIVANQLMDSISTAEELTAAGDLVLYDQLGAAQVDDKFPVFTPM
                     AVSVQGSVNLGRGFFLQSLIVQNTAPVHEQMIARPNMVALIPQFDTRLVTVSVPVSVY
                     DYEEVHVGTSLRVGWFTVGTDNLPSLLIPGDLDGADIYAGIRVMPWKHAGAAAAIGGA
                     SGRKARSRMLDCPDVF"
     gene            complement(3435..4814)
                     /locus_tag="PK742_07540"
     CDS             complement(3435..4814)
                     /locus_tag="PK742_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQU76482.1"
                     /translation="MSELVSIVGDGYVLTSPEILESYSHDETEDLHFPPAAVIRPANT
                     EEVAAIMKLASLYKIPVTPMGARTGLSGGALPVFGGIGLSMERFDRILRIDERNHQVT
                     VQPGVITQVLQEAVAEKGLFYPPDPASRGSCFIGGNVAENSGGPKAVKYGVVKDYVLN
                     LEVVLPSGEVIMTGANVLKNATGYNLTQLMVGSEGTLGVVTAIVLRLIPLPAFDKLLF
                     IPFPDATQACAAVSAVFRAGIIPSAMEFMERAAVELVCRYHPELIIPLPDKAAAFLLV
                     EVDGNHEDLLVQDCASISTVVEAFGAGEISFAESDTEKTKLWSIRRLAGESVKRHSVY
                     KEEDTVVPRAELPALLENVKSIGRAYGFESVCYGHAGDGNLHVNILRGDLSAEAWEEK
                     LPVAIRELFTEVVKLGGTISAEHGIGYVQKPYMPIAFSEIQLEIFRRIKKVFDPHNIM
                     NPGKVIDMM"
     gene            4950..5240
                     /locus_tag="PK742_07545"
     CDS             4950..5240
                     /locus_tag="PK742_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76483.1"
                     /translation="MKKVYALGMAAILVMASVTTFGQAKKYPLFEHFTQASCGPCAAQ
                     NPYFQAVYEENVTNVHHVAYHTSWPGYAQCMNTTQPKAKRWSVFTAFQVYLP"
     gene            5168..6994
                     /locus_tag="PK742_07550"
     CDS             5168..6994
                     /locus_tag="PK742_07550"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76484.1"
                     /translation="MYEYNTAESEAMVSFYSVSGVPTMILDGADIGSPAGVTQDMIID
                     EGINGSPIRILVTEETAGTTRNVHVEVQTVQDIPAGTYRIKGAVAERLIEYGSAPGSN
                     GETEFPNVFRQGLISGTTGDIITLAPVGSSVSFDYSYELDPLWQEDEIYALVWVQNSS
                     SKEVLNSGAPGDPPLEYVNTSSVLFTQGGGSFGSIVNNIGDIDEGFTVTLTVEQPADW
                     SASYTYGGSTYSGAFSTSATAGELEDLTLNVETGTTPGVGFYTLTLDPDHEGWSNMII
                     KYYTVNGVTDLVLNNVTSFGDGSPYGTWNFESMYTDGLDLAENTSYGVAGHNVFIAGM
                     KGDAFGELKNIYFNVGWTFPSLLNDELRNYLTDFMDNGGNLFISGQDIGWEADYYATE
                     FGLTGALNFYENYLHSVFVNDGATGAGTLTAIADDEVFGTVAESNIIDDVYGSSYFYP
                     EQIDVADADGHAIFSYGGNDAKIAGVRSETSDNKTVYLGIGIEQIEDEAIRSQVVKLS
                     HDYFYGLVNGIEFDAAIASLMGSASPNPASNATSVSFNGLDHNVSLVITSLTGQVIKT
                     LQVPAGTGNYEIDVTDMESGMYLYHITDGQSQSPAQKLNVIK"
     gene            7005..>7511
                     /locus_tag="PK742_07555"
     CDS             7005..>7511
                     /locus_tag="PK742_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76485.1"
                     /translation="MRRLLGVLSLAILAVTAVQAQSYITLSADTSYATGSPDEIHKAE
                     VVVTNNGPDGQNMTWVRVVNDKPEEWETSVCDPNLCWAPFADAPGYGWFQPAGADDDF
                     YVQFDGRNFPDGPAVDGHGEVEVIIYSEDDSANYNARGVFIADLSGEVGFHSPDMDNQ
                     YEVYPNPAV"
BASE COUNT         1889 a         1860 c         1853 g         1909 t
ORIGIN      
        1 gcatcgcggc gcccacggcg gcaccggtga aggccatctc cgactttctg ggccttgatg
       61 tgcacgacat aggcaatcac tacgagcctg ttccggttgg cgctgtcatg acttgtgagc
      121 ccggcattta cattcgtgaa gaaggcattg gtatccgtat tgaaaacgac gttatgatca
      181 ccgagaacgg tctgtatgat ttcatgcgtg atttcccgcg ggaagtagaa gagatcgaag
      241 acatcatgaa cagccggaac tgatacggat gaaactgatc aggcatctac ccaatttgct
      301 gaccctgacc aacctgtttc tgggttgcat ggccattgtt tacatctact atgatcattt
      361 gctcattgtg gatgccaagc agaatgtcta tgccgatatg ggaaggatgg aaatggctgc
      421 tttttgtgta tttgcagctg cgatcattga tttcttcgat ggttttgtag ccaggttact
      481 gggtgttact tcttccatcg gaaagcagct tgactcactg gcagatatgg tcaccttcgg
      541 tctggtaccc ggactgatca tgtaccagct cattgcccgt tcctattatg tttctgccga
      601 agcattcgac tatccgatca tgtatttcac cagcggattc tttctgaccc ttatggccgc
      661 tgtcagactg gcaaggttca acacagaacc ggaaacagcg ggtttcagcg gacttccttc
      721 cccttcaatg gccttattcg tagtgtcact acccctgatc attttcagaa atgatgcagg
      781 gctggcgagc atgctgacca ataaatgggt gttgctgctc attaccctgt tgctgggtta
      841 tctgatggtg agcagactgc ctatgccggg tctcaaactg aaaggttttg cctgggaaga
      901 caaccgatgg tcctacatca ttttcctgac cggactgata ttcgggctgg ggggaattct
      961 cctgttcgga cttacctttg cagtgatccc gctggtgatc attgtgtact ttattctctc
     1021 gctgataaaa aacatctctg aaaatggcat atagagccga aattgatatc atgcccctga
     1081 aagaactgct ggatccgaaa ggcaaggcag tggccaacaa tatgaaaagt ctgcaactgg
     1141 ctgcagtcaa agacgtgcgc atcggtaagc acatcacact ggagatccag gccggttctg
     1201 aagaagaagc ccgcaccatg accgagaccg cctgcaaaaa actgctggcc aatccgatca
     1261 tggaatccta cacctttgag gtattcccga tgtgagtaaa ctctaccttg ttcccacccc
     1321 catcggcaac ctggccgata tgacctaccg ggcggtggag gtattgcgca gcgctgatct
     1381 gatcctggcg gaagacacca gaacctcagg tgtgctgctt aaacactatg gcatcgacac
     1441 gaagatgcgt tcctatcacg cttttaatga gcatgagacc gtcgggcgac tggcttcaga
     1501 aatagcctca ggtgtcacga tggcacttat caccgatgcc ggcacgccgg gtatctcaga
     1561 tccgggctac ctgttggttc aggcttgtat tcagcagcaa attcctatcg aaaccttacc
     1621 cggagccact gcatttgtac ctgcactggt gaacagtggc ctgccggcgc atgaattcac
     1681 ttttaccggt ttccttcccc ccaaaaaagg ccgggaaacc cgattgaaaa agctggctga
     1741 agaagaacgc accctgatcc tgtatgaatc gccacacaga ttgatgaaga ctctggaaca
     1801 atgtctgcag ttctttggcg aacgtccggc cagtgcttcc cgcgagcttt ccaaaaaatt
     1861 cgaagagacc gtaaggggat ctttgaccga tctgatcacg cacttcagcg agcaccctcc
     1921 caagggagag ttcgtactgg tgatcggcgg aaaggaagca gaaaaagtca aaaaacgtca
     1981 ggacaatcca gcatcctaga tctggctttt ctgccggaag caccccctat tgcagctgct
     2041 gctcctgcat gcttccacgg catgacacgg atacctgcat aaatatctgc tccatccaga
     2101 tcaccaggta taagaagtga cggcaggtta tcggtaccta cagtaaacca tcccacccgc
     2161 aaggaggtgc ccacatgcac ttcttcatag tcatacacag ataccggaac agatacggtt
     2221 accagtctgg tgtcgaactg cgggatcagt gcgaccatat ttggtctggc gatcatttgt
     2281 tcgtgtacag gtgcggtatt ttgcacgatc agtgactgga ggaagaatcc tcttccgagg
     2341 ttcacagagc cctgtactga aacagccatc ggggtaaaga ccggaaattt atcatccacc
     2401 tgagcggcgc caagctggtc atatagcaca agatcacctg cagcagtcag ttcctccgca
     2461 gtgctgatac tgtccatcag ctgattggct acaatgacct gatcattggc cgcaagatga
     2521 taacttccgg caccatcacg gaaacggatg aaacccatat ctaccacact cacaccgact
     2581 ttccagttgt aggctgcata ctctttcctg cttctggaaa ccggttgcat ccaggtaaat
     2641 ccgagatcag cgcccaggcc ccagccattc aggttgctct gatagagtcc attttcggga
     2701 taaccccctg cattgactga ataactgtag tcaagtaata gattggtaag ctcaacatca
     2761 cgatctgtgc gggtgaaagt gaatgcttcc cggttgtgtg cagtgatggc atcaaaccca
     2821 cccaggtatt taatgttcgc acctacatgc aggttccgcg taagtgccat acctacattg
     2881 gcaccaaatt ccatccagtt cagcaaacct aaatcaacag tgccaacctg taccggtaca
     2941 tcttctatga tattttccag cacattgaca ccttccggaa agttgccgga gtggacatat
     3001 cctgcagatc tggctgtggt gaacaaacca aaagaaaaat aacctgtatt gataaagccg
     3061 gacgggcctt gtaccagcat attagaatgg gcattgacat agtctgtggt aaagaaagca
     3121 gtttctgcat cctgtgcaga cagcaaaccc acagcactct ggccaggtgc atatatataa
     3181 ttgttttgtg caaaggcacc ggcactcacc aggttgacat cccagggcag attgccactt
     3241 aacagtgaag aagggttgag cagcatgccg ttggagccgc tgaaatttcc tgtgcgcaag
     3301 cctgtaaatt cctgtgctga ggccggccat gccagcaaca gcattgcata taataagatg
     3361 gttctcatac aaagtcttgt cggacgctgc cggtgtgcgg ggaagaataa gaagcagggt
     3421 tccgggtaca ggtgttacat catatcaata accttgcccg ggttcatgat attgtgtgga
     3481 tcgaagactt ttttgatccg gcggaagatt tccagctgta tttcgctgaa agcaataggc
     3541 atatatggtt tctgcacata tccgatgcca tgttcggcag aaatggtgcc gccaagtttc
     3601 accacttcgg tgaacagttc ccggatggcc acgggaagtt tttcttccca agcctcagct
     3661 gacagatcgc cacgcaggat atttacgtga agattgccat cgcccgcatg gccgtaacac
     3721 acactttcaa agccataggc acggccaatg gacttcacat tctccagcag agcgggaagt
     3781 tcggcacggg gtacaacggt atcttcctcc ttgtacacag agtgcctttt gaccgattcg
     3841 ccggcaagtc tgcgaatgct ccacagcttt gtcttttctg tgtcagattc agcaaacgaa
     3901 atttcgccgg ccccgaaagc ttccactact gtggaaatac ttgcacaatc ctgtaccagt
     3961 aagtcctcat ggttgccatc tacctccacc agcaggaagg cagcagcttt gtctggcagg
     4021 ggaatgatca gctccggatg gtacctgcat accagttcca cagcggcacg ttccatgaat
     4081 tccatggcac tgggaatgat gcccgcccgg aaaacagcag aaactgctgc acaggcctgc
     4141 gtggcatccg gaaacggaat gaacaggagt ttatcaaatg cgggcagggg gatgagccgc
     4201 agaacgatgg ccgtgaccac acccagggtc ccttcactgc ccaccatcag ctgggtcagg
     4261 ttgtatcctg tggcattctt gagcacattg gcaccggtca tgatgacctc gccggaaggc
     4321 aatactactt ccaggttcag cacatagtct tttaccactc cgtatttcac tgctttgggt
     4381 ccgccggagt tttcagccac atttccgcca atgaaacaac ttcctctgct ggcaggatca
     4441 ggcggataaa acaggccttt ttcagccact gcttcctgga gtacctgggt aatgaccccg
     4501 ggctgcacgg tgacctggtg gtttcgctca tctatgcgga ggatgcgatc aaagcgttcc
     4561 atgctcagac caattcctcc gaacacaggt aaggctccgc cactaagacc tgtccgggca
     4621 cccatgggag taacggggat cttatacagg gatgccagct tcatgatggc agccacttcc
     4681 tccgtattgg cgggacgaat gacagctgcc gggggaaaat gcaggtcttc tgtttcatca
     4741 tgcgaatagg actccagtat ttcgggagag gtcaacacat atccgtcacc aacaatggat
     4801 accagttcag acatcagatg ctgcgaaagt tgaccatatg tggctattcc tgacattgca
     4861 atggcaaagt aagtgggtta gcggcagaaa attggtagtt ttgttgattg taccttaacc
     4921 taaatatcat caataactat ttgccaatta tgaaaaaagt ttacgcatta ggtatggctg
     4981 ccattctggt aatggcatct gttaccactt tcggacaggc taaaaaatat cctctgttcg
     5041 agcatttcac ccaggcttcc tgtggtcctt gtgctgccca gaatccatac ttccaggcag
     5101 tttatgagga aaacgttacc aacgtacacc atgtggcgta tcatacatca tggcctggct
     5161 atgcccaatg tatgaataca acacagccga aagcgaagcg atggtcagtt tttacagcgt
     5221 ttcaggtgta cctaccatga tcctggacgg tgctgacatt ggcagtcctg ccggtgttac
     5281 tcaggatatg atcattgatg aaggtattaa cggcagtcct atccgaattc tggtaacaga
     5341 agagactgcc ggtaccacca gaaatgtgca tgttgaggtg cagaccgttc aggatatacc
     5401 tgccggcacc taccggatca aaggagctgt tgctgagcgt ttgatcgaat atggttctgc
     5461 tccgggttcc aacggagaga ccgagtttcc gaatgtattc cgtcagggtt tgatatcagg
     5521 tacaaccggc gatatcatca cccttgcacc ggttggttct tctgtttctt ttgattacag
     5581 ctatgaactg gatcccctct ggcaggaaga tgagatctat gcccttgtat gggttcagaa
     5641 ttcttcttcc aaagaggtgc tgaactccgg agctcccggc gatccgcctc tcgaatatgt
     5701 caacacctca tccgtgctgt ttacacaggg tggtggttct tttggctcca ttgtcaataa
     5761 tattggcgat atagatgaag ggtttactgt gacgcttact gttgagcaac ctgcagactg
     5821 gtcagcttcc tacacttatg gtggttctac ctacagcgga gctttcagca catctgcaac
     5881 cgccggtgaa ctggaagacc tcaccctgaa tgtagaaacc ggtactaccc caggtgttgg
     5941 attctatacc cttaccctgg atcctgacca cgaaggctgg agtaatatga tcataaagta
     6001 ctatacagtg aacggtgtta ccgatctggt actgaacaac gtgacttctt ttggtgatgg
     6061 atctccttac ggtacctgga attttgaatc catgtacacc gatggccttg acctggccga
     6121 gaataccagt tatggtgtag ccggacacaa tgtgttcatt gccgggatga agggagatgc
     6181 ttttggcgaa ctcaagaaca tatacttcaa tgttggctgg accttcccgt cactgttgaa
     6241 tgatgaactg cgcaattatc tgacagattt catggataac ggtggtaacc tctttatttc
     6301 cggacaggat attggttggg aagctgatta ttatgcaact gaatttggtc tgacaggtgc
     6361 tctgaatttt tacgaaaact acctgcactc tgtttttgtg aatgatggcg caacaggtgc
     6421 cggtaccctt acagcaattg cagatgatga agtatttggc accgtagcag aatccaatat
     6481 catagatgat gtgtacggca gctcttattt ctatccagag cagattgatg ttgctgacgc
     6541 tgatggccat gccattttct catatggtgg caacgatgcc aaaatagcag gcgtgcgctc
     6601 tgaaaccagt gataacaaga ccgtatatct gggtattggt attgagcaga tagaagacga
     6661 agctatacgc agccaggtgg tcaagctcag ccatgactat ttttacggcc tggtaaacgg
     6721 tattgagttt gatgcagcta tagcttctct gatgggaagc gcttctccaa accctgccag
     6781 caatgccacc agcgtttcat tcaatggttt ggatcacaac gtaagtcttg tcatcacatc
     6841 tttgaccgga caagtgatca agacccttca ggtacctgcc ggtacaggca actatgaaat
     6901 tgatgtgacc gatatggaat caggcatgta tctgtatcat attacagatg gccagagtca
     6961 gagtccggca cagaaattga atgtaatcaa gtaatcagac aacaatgaga cgacttttag
     7021 gtgttttatc attggccata ctggccgtca ctgcagtgca ggcacagagc tatattactc
     7081 tgagtgctga cacttcatat gctaccggtt ctcctgatga gatccacaaa gctgaagttg
     7141 tagttaccaa taacggtcct gatggtcaga acatgacctg ggtgcgtgtg gtgaacgaca
     7201 agccggaaga gtgggaaact tcggtatgtg atccgaacct ctgctgggca ccatttgctg
     7261 atgcccctgg atatggatgg tttcagcctg ccggagcaga tgatgacttc tatgtgcagt
     7321 ttgacggtag aaattttcct gacggtcctg ccgtggatgg acatggagaa gttgaagtca
     7381 tcatctacag cgaagatgac tctgccaact ataatgcccg tggggtattc attgctgatc
     7441 tgagcggtga ggttggtttt cattcgccag acatggacaa ccagtatgag gtctatccga
     7501 accctgctgt a
//
LOCUS       DAOMAX010000121         7402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1518_length_7402_cov_4.048455, whole genome shotgun sequence.
ACCESSION   DAOMAX010000121 DAOMAX010000000
VERSION     DAOMAX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1518_length_7402_cov_4.048455"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PK742_07560"
     CDS             <1..765
                     /locus_tag="PK742_07560"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76486.1"
                     /translation="VAVLNGNSESSLYPTAPEHAGVEGSVIILDSALNIISQKQWGGT
                     LDDFLVTGTQDSVGNFYFLGASKSSDYDVIENHYGGEELNYWILATDSSFNPLWSKSF
                     GGGNNNCGDLCGGVFTGTLLVKDSILYAFTESVSPEILPDYDIECGHYNASTPKSDAW
                     LVAFDLTTVIPEQEVAQTPFTIYPNPTSDVLFVHCASDNSTISYTIRSLSGSILKTGT
                     RNGNEGISVDLLPSGFYFITMETDEHQYFVSTFCKI"
     gene            1011..2084
                     /gene="prfA"
                     /locus_tag="PK742_07565"
     CDS             1011..2084
                     /gene="prfA"
                     /locus_tag="PK742_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQU76487.1"
                     /translation="MDLLSKLRSIKERYELLQEQLSDPELIADQAKYKRLNKDYKDMQ
                     PVVEAFDRYELVLGNMEDSRRIIQTEKDADLKDMAKEELELLQTEQTELEERIRILLL
                     PKDPEDAKDAILEIRAGTGGDEAALFAGDLFKMYQRYCDAKGWKTDVINMNESEQGGY
                     KEIVMEVAGENVYGTLKYESGVHRVQRVPATESQGRIHTSAATVAVLPEAEEVDVQLN
                     MNDVRVDTFRASGAGGQHVNKTESAIRLTHIPTGLVVECQDERSQMKNKDKAIKVLRT
                     RLYERELAAHHAKIASARKTLVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLPVVMN
                     GQIGEILDALQTAENAEKMKSGG"
     gene            2497..3405
                     /gene="gldA"
                     /locus_tag="PK742_07570"
     CDS             2497..3405
                     /gene="gldA"
                     /locus_tag="PK742_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HQU76488.1"
                     /translation="MSISVQHLTKIYGEQRAVDDISFEVPAGQIVGFLGPNGAGKSTT
                     MKILTCFIPPSAGEAKVCGFDGMKDPIEVKKRVGYLPEHNPLYPDMYVQEYLEFAARM
                     QGMKKFGERVEEMIAVTGLSPERKKKIGQLSKGYRQRVGLAQAMLHDPEVLILDEPTS
                     GLDPNQLADIRHLIKTLGQKKTVILSTHIMQEVQALCDRVIIINKGRIVADDSVEGLQ
                     ARSSGATIVQVSFKESPDKDLFRKIPGVAEVKRIDDQQFLIQYIGQEDIRETLFKFAV
                     DNKLTLLNLQLEKQSLEDVFRQLTGR"
     gene            3606..4337
                     /gene="gldF"
                     /locus_tag="PK742_07575"
     CDS             3606..4337
                     /gene="gldF"
                     /locus_tag="PK742_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HQU76489.1"
                     /translation="MVAIFRKELNVFFSSLIGYIAITIFLVANGLFNWVFTDTSIMES
                     GYANLEPFFNFAPWIFLFLVPAITMRSFAEEKNSGTLELLATRPVTDLQIIGGKFLAA
                     FVLVLFSVLPSIIYYFAIRELASPKGNVDTGGIIGSYIGLTLLGGCFVAISLFASAVS
                     NNQIVAFISGLFLCFFLYYGFDALALLPVSEGSADAVFNAISINQHYQNISKGYIDFR
                     DVLYFGSAVSIFFLMTQTALGSRRW"
     gene            4334..6046
                     /gene="gldG"
                     /locus_tag="PK742_07580"
     CDS             4334..6046
                     /gene="gldG"
                     /locus_tag="PK742_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HQU76490.1"
                     /translation="MNEVSISSGFERNRRRAMLRLLLLVLIIVLLNVVLSRVFFRLDL
                     TQEKRYSLSTPTKELLEEQSDIVFVTIYLAGDLTPNMQQLQTSTVEMLKQFKAYGHDN
                     IQWEIQNPFDITDEVNLGKFIQELEDRGVYSIPFGSADKEQTSRSFLFPYANVNYKGK
                     DLSLLLLDQVQPLSPLYDPTSAISLLEYKFSKSIRLLSETKRPDIAFIEGQNELDTLE
                     VQDIAISLSELYNMYRIDLPNSDYIDTLYDVVVIAKPRSAFSEEDKYKLDQYLMYGGR
                     ILWLIDPVIAEFDSLRSTGEFTSVDYPLDIMDQLIQYGARVNNNIIQDKQCSHIKVPY
                     GNTFQDRPWLYNPVITGFNKQHPISANIDAVEAKFVSSIDTTAVPGIRKTILLTTSAN
                     SRFLMSPARINYNIVQSQYMPTDEQFNKPNLPVAVLLEGEFPSAFSTRKPTAKAMERR
                     RLGNGPYAERSIPTKQIVVSDGDMIRNYVDADGKIYPLGANNIERYIFGNKDFLMNCI
                     EYLTDNTGLMETRAKEVKLRPIDKEQVEANRTQWQLINIGAPVLLIYLFSGIYNYIRN
                     RKYA"
     gene            6043..7122
                     /locus_tag="PK742_07585"
     CDS             6043..7122
                     /locus_tag="PK742_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HQU76491.1"
                     /translation="MKKNLIYLAVVIVLAILAWFFIFNQGTGSFGGDDKAFGVKDTAS
                     IGKIFLADLSGNKIEVERSGDGWVMDDGTPVRQEVMNTILLTFNQMEIKAPVAKSMFN
                     KVIRDIAGNHIKVEVYTRGGKQIRSYFIGPIAANGRGNYMLVEGSKTPFELQIPGFDG
                     FISSRFDMQVINWKDRTVFKYRPDNIAKLEVNYPRVPDSSFVIERSADGQYTFINRST
                     DVRQFNPEIGNYYLNQFKRLNCELYLDDKYKMDSLASQQPVCVMTVTDLDGNSRSAEV
                     FYRPVSYRTKMQFTYEGKEINFDMDKYYAIINDGKDLSIIQNFVFGKLFIGPSYFYRQ
                     RGETPNVLIQDLMQDSEGETVDLME"
BASE COUNT         1919 a         1890 c         1918 g         1675 t
ORIGIN      
        1 gttgccgtat tgaatggtaa ttctgagagt tcgctgtatc ctacagcccc cgagcatgct
       61 ggcgtagagg gttctgtaat aatacttgat tctgccttaa acatcattag ccagaaacag
      121 tggggcggaa ccttagatga ctttttagtt acaggcactc aggattcggt aggtaatttt
      181 tattttttag gtgcaagtaa atccagcgat tatgatgtga tagaaaacca ctatggtggc
      241 gaggaattaa actattggat actagccaca gattcttcat tcaatccctt atggagtaaa
      301 tcatttggcg gaggtaacaa taattgtggt gacctgtgcg gaggagtttt tacaggcacc
      361 ttattggtca aagacagcat attgtatgcc tttaccgaaa gtgtatctcc tgaaatactg
      421 ccggattacg atatcgaatg tgggcactat aatgcatcta ctcccaaatc agatgcctgg
      481 ctggtagcct ttgacctcac taccgttatt ccggagcaag aggtggccca gacacccttt
      541 accatctacc ccaatcccac atctgatgta ctttttgtcc attgcgcatc agataattca
      601 accatatcct atactatcag gtccctctct ggcagtatat taaaaaccgg tactcgtaat
      661 ggaaatgagg gcatctctgt cgatctgctt ccatctggat tttactttat cacaatggaa
      721 actgacgagc atcagtactt tgtgagtacg ttctgcaaga tctgatcggg atgaacctga
      781 cctccattgt gctggaataa ttaaggacac aacgaagctg cacaggagat tcacagcccc
      841 gattgcagca ggtagcccgg acccctgccg atagttatcg ggagctatcg ggagcgaact
      901 tagggggagg actactgcgg aaagcgggac caacgttgct ggatggcaac aggatgcttg
      961 ctccaactat cctgtcagac cataccgctc ttccttacct ttgcacacgc atggaccttc
     1021 tttccaaact ccgatccatc aaagagcgct atgaattgct gcaggagcaa ctgagcgatc
     1081 cggaactcat tgccgatcag gcgaagtaca agcgtctgaa caaggactac aaggacatgc
     1141 agccggtggt ggaagccttt gaccgctatg aactggtgct gggaaatatg gaagacagcc
     1201 ggcgcatcat ccagaccgaa aaagacgctg atctgaaaga catggccaaa gaggaactgg
     1261 agttgttgca gaccgaacaa acggaactgg aagaacgcat ccgcatcctg ctgctgccca
     1321 aagatccgga agatgcgaaa gacgccatcc tggagatccg tgcgggtacc ggtggcgatg
     1381 aagctgccct ctttgccggc gatctgttca agatgtacca gcgctactgc gatgccaaag
     1441 gctggaagac cgatgtcatc aacatgaatg aaagtgaaca gggcggatac aaggagatcg
     1501 tgatggaagt ggccggcgaa aacgtatatg gtactttaaa atatgaatcc ggtgtgcacc
     1561 gggtgcaacg ggtgcctgcc accgaatcgc agggacgcat ccatacgtcg gctgccaccg
     1621 tggctgtgct gccagaagcc gaagaggtgg acgtgcagct caacatgaac gatgtgcggg
     1681 tagatacctt ccgggcatcc ggtgccggtg gtcagcacgt gaacaaaact gaaagtgcca
     1741 tccgcctgac gcatattcct accggactgg tggtggaatg tcaggacgaa cgttcacaga
     1801 tgaagaacaa ggacaaggcc atcaaggtgc tgcgtacccg gttgtatgaa agagaactgg
     1861 cagcgcatca tgcgaagatc gccagtgcca ggaagacact ggtcagcaca ggtgatcgtt
     1921 ctgccaagat ccgcacgtat aattatcccc agggccgtgt gaccgatcac cgcatcaacc
     1981 tgacgctgta caacctgccg gtggtcatga atggtcagat cggcgagatc ctggatgcgc
     2041 tgcagacggc ggagaatgcg gagaagatga aaagcggcgg gtgaaaggcg gaggacggaa
     2101 tgcggaaagc ggaatgagtg aggggtgagg cagtgaagca gtaagcagta gagcagtaag
     2161 cagttgaaga gcaaacagtt taggtgagcc cctccctata gctatcgggt tcaattggga
     2221 ttggcagttg gcggttggtg gtaaaacaat ttagaagact ctttgcgtcc tctgtgtttg
     2281 tttcggcgaa gccggaacct gcgcctttgc gttcaaaaaa tccagggaca ttatatgtga
     2341 ccggtgaatc cctgtcggga agtgaggggt gagatgtaca ccattcaggg tatgaaccat
     2401 tcactcccga tcgttatcgg gttcactttc cgcctatggc ggaattgacc gctcactcgc
     2461 agcttcattc tccaattacc cctatcttcg tgccgcatgt ccatcagtgt gcaacatctc
     2521 accaagatct acggcgaaca gcgggctgtg gatgatatca gcttcgaggt tccggccgga
     2581 cagatcgtcg gctttctggg tcccaacggc gccggaaaga gcacgaccat gaagatcctg
     2641 acctgcttca ttccgccctc tgcaggcgaa gcgaaggtgt gcggatttga cggcatgaag
     2701 gatcccatcg aagtgaaaaa aagagtggga tatctgccgg agcacaatcc gctctatccc
     2761 gacatgtacg tgcaggaata cctggagttc gctgcccgca tgcagggcat gaagaagttc
     2821 ggagagcggg tggaagagat gatcgcggta acgggattgt cacccgagcg taaaaagaag
     2881 atcggtcagc tgagtaaagg ttatcgccaa cgggtaggac ttgcacaggc catgctccac
     2941 gaccccgaag tgctcatcct ggatgaaccc acctccggcc tcgaccccaa ccagctggca
     3001 gacatccgcc accttatcaa gaccctcggc caaaaaaaga ccgtgatcct gagtacgcat
     3061 atcatgcagg aagtgcaggc cttgtgcgac cgggtcatca tcatcaacaa aggtcgcatc
     3121 gtggccgacg acagtgtgga aggtctgcag gcccgcagct ccggcgccac cattgtgcag
     3181 gtcagcttca aggaaagccc ggataaagac ctgttccgca agataccggg tgtggcagaa
     3241 gtgaagcgta tcgacgatca gcagttcctg atccagtaca tcgggcagga ggatatccgc
     3301 gagaccctct tcaagtttgc cgttgacaac aaactcaccc tgctcaacct gcaactcgaa
     3361 aagcaaagcc tggaggatgt gttcaggcag ctgacgggga gatgaggtca gttcacagtt
     3421 ggcagtaagc agttggcagt caaactccat tcctcccgat agctatcggg aaagaggacg
     3481 aaaagcgaaa caggttggtt ctgacccccg gtttcagtag ttcacattcc gcatttcgaa
     3541 ctccgtactc cgctttgttc ccagaccaat gtgctacttt tgccgtttag tgcaaaagtc
     3601 ccggagtggt agccattttt cgtaaagaac tcaacgtttt cttcagcagc ctgatcggct
     3661 atatcgccat caccattttc ctggtggcga acggcctgtt caactgggtg ttcacagaca
     3721 ccagcatcat ggagagcggt tacgccaacc tggagccttt tttcaacttc gctccctgga
     3781 tcttcttatt tctggtaccg gccatcacca tgcggtcttt cgctgaagag aagaacagcg
     3841 gcaccctcga actgctggcc acccgaccgg tgaccgacct gcagatcatc ggcgggaaat
     3901 tcctggccgc attcgtcctg gtactgttca gcgtgctgcc cagcatcatc tattatttcg
     3961 ccatccgcga gctcgccagt ccgaagggca atgtggacac aggaggtatc atcggttcgt
     4021 atatcggact caccttatta ggcggatgct ttgtagccat cagcctcttt gcatcggccg
     4081 tgagcaacaa ccagatcgtg gctttcatct ccggtctgtt cctgtgcttc ttcctgtact
     4141 acggctttga tgccctggcc ttgctgccgg tcagcgaagg cagtgcagat gcggtgttca
     4201 atgccatcag catcaaccag cattatcaga atatcagcaa aggctatatc gacttccgcg
     4261 acgtgctgta tttcggcagc gccgtgtcca tattctttct catgacccaa accgcactcg
     4321 gcagcagaag atggtgaacg aagtttccat atcatccggg tttgaacgga accgtcgccg
     4381 ggcgatgttg cgtctgttgc ttttggtgct catcatcgta ctgctcaatg tggtactgag
     4441 cagggtcttc ttccgccttg acctcaccca ggaaaaacgc tacagcctca gcacacctac
     4501 caaggaactg ctggaggaac aaagcgatat cgtcttcgta actatttatc tggccggcga
     4561 tctcaccccg aacatgcagc aactgcagac ctccacggtg gagatgctca agcagttcaa
     4621 ggcatacgga cacgataata tccagtggga gatccagaat cctttcgaca tcaccgatga
     4681 ggtcaacctg ggaaaattca tccaggaact ggaagaccgc ggcgtctatt ccattccttt
     4741 cggttctgcc gataaagaac agaccagccg ttcctttctt tttccatatg ccaacgtcaa
     4801 ctacaaaggc aaggatctga gcctgctgtt gctggaccag gtgcagccat tgtcgccgct
     4861 gtatgatcca acatcagcca tctcgctgct cgaatataaa ttctccaagt ccattcgtct
     4921 gctcagcgag accaaacgtc cggatatcgc attcatagaa ggacagaacg aactggacac
     4981 cctggaagtg caggatatcg ccatttcgct cagtgagttg tacaacatgt atcgcatcga
     5041 tctgcctaat tcagattata tcgatacgct gtatgatgtg gtggtgatcg ccaaaccgag
     5101 aagcgccttt tcagaagaag acaaatacaa actggaccag tacctgatgt atggcggaag
     5161 gatactctgg ctcatcgatc cggtcatcgc agaatttgac agcctgagaa gcacgggaga
     5221 gttcacttct gttgactatc ccctcgacat catggaccag ctcattcagt acggagcgag
     5281 ggtcaacaac aacatcatcc aggacaaaca gtgcagccat atcaaggtgc cttacggcaa
     5341 caccttccag gacagaccct ggctgtacaa ccccgtcatc accggattca acaagcagca
     5401 tcccatcagc gccaacatcg atgcggtgga agcgaaattc gtgagcagta tcgacaccac
     5461 tgctgttccg ggcatcagga agaccatcct gctgaccact tcagccaaca gccgttttct
     5521 gatgagtccg gcacgcatca actacaacat cgtacagagt cagtacatgc caaccgatga
     5581 gcagttcaac aaaccgaacc tgccggtggc tgttttgctg gaaggcgaat ttccttccgc
     5641 cttcagcacc cgtaagccca ctgccaaggc gatggaacgc agaagactgg gcaatggtcc
     5701 gtatgcagaa cgcagcatcc ccaccaagca gatcgtggtg agtgatggcg atatgatcag
     5761 gaactatgta gatgccgacg gcaagatcta tcccctgggt gccaacaaca ttgagcgcta
     5821 catctttggc aacaaggatt ttctgatgaa ctgtatcgaa tacctgaccg acaacaccgg
     5881 cctgatggaa actagggcca aggaggtcaa gcttcgtccg atagacaagg agcaggtaga
     5941 agccaaccgc acgcagtggc agctgatcaa catcggcgca cccgtattgc tgatctatct
     6001 gttcagcggc atctacaact acatccgcaa ccgtaagtac gcatgaaaaa gaatctgata
     6061 tatctggcag tggtgatcgt gctggccata ctggcctggt tctttatttt caatcagggc
     6121 acgggcagtt ttggtggtga tgacaaagcc tttggcgtga aggatactgc cagtatcggc
     6181 aagatattcc tggccgatct gagtggcaat aagatagaag tagaacgcag cggcgatggc
     6241 tgggtcatgg atgacggaac tcctgtgagg caggaggtga tgaacaccat cctgctcaca
     6301 tttaaccaga tggagatcaa ggcaccggta gcgaaatcca tgttcaacaa ggtgatccgc
     6361 gacattgccg gtaaccacat caaggtggag gtatatacca gaggtggcaa gcagatacgt
     6421 tcgtatttta tcggccctat tgctgccaac gggcgtggca attacatgct ggtggaaggc
     6481 agcaagacgc cgttcgaatt gcagataccc ggttttgacg gattcatctc ctcccgtttc
     6541 gatatgcagg tcatcaactg gaaagaccgc acggtattca aatacagacc ggacaatatc
     6601 gcgaagctgg aggtgaacta tccccgtgtg cccgacagca gttttgtgat cgaacgcagt
     6661 gctgatggtc agtacacttt catcaacagg agtacagatg tacggcagtt caatccggag
     6721 atcggcaact attatctcaa ccagttcaag cgactgaact gtgaactata cctcgacgat
     6781 aaatacaaga tggacagcct ggcctctcag cagccggtct gcgtcatgac cgtgaccgac
     6841 ttagacggca acagtcgcag tgcggaagtg ttctaccggc ctgtctccta tcgtaccaag
     6901 atgcagttca cttacgaagg caaggagatc aacttcgaca tggacaagta ttacgccatc
     6961 atcaatgatg gtaaagacct gtccatcatc cagaacttcg tattcggcaa gctgttcatc
     7021 ggcccgtcct acttctaccg ccagcgtggt gaaacgccca acgtgctcat ccaggacctg
     7081 atgcaggatt ctgagggaga gacggtagat ctgatggagt agttggcagt taacccctgc
     7141 gggggcagtt ggcggttggc agttttcaaa aaaaaattat gggaattgag atatgttctc
     7201 ccttcgatct gattgttatc ggaatttaca tgttaatgat ttctctcctg ctggtagaat
     7261 taaaccataa aaatagacgg aagggtttta acacaaaggc acaaaggagt cgcaaaggac
     7321 gcagagggtc ttcaaatatg cttcactatt ctatagcctt gttgaacgga tgattcattc
     7381 accattgact tcccgctttc ag
//
LOCUS       DAOMAX010000122         7395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1519_length_7395_cov_4.207221, whole genome shotgun sequence.
ACCESSION   DAOMAX010000122 DAOMAX010000000
VERSION     DAOMAX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_7395_cov_4.207221"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..420)
                     /locus_tag="PK742_07590"
     CDS             complement(88..420)
                     /locus_tag="PK742_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQU76492.1"
                     /translation="MEFREFNAYVYILTNKTRSVLYIGVTNSLERRVFEHKVRLHPDG
                     FAARYECDRLIYFEGYSDMSNAIIREKQLKKWKRGWKTALIDESNPPWLDLAEDWYSD
                     EDFKDFID"
     gene            588..2585
                     /gene="rpsA"
                     /locus_tag="PK742_07595"
     CDS             588..2585
                     /gene="rpsA"
                     /locus_tag="PK742_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQU76493.1"
                     /translation="MTTENENMELENNSETTVTEEVTETSTKAETSATESKDQPAFDD
                     FDWGVDKRNRTSYATDKAEAMEKEFEATFRTLQDDEIVLGTVVGITDSDVVMNVGYKS
                     DGLIPRTEFRDIEDLKVGMEIDVYVVSKEDTKGHLVLSRKNAKMMQAWDKIVDAYNNE
                     TVVTGNIVSKTKGGLIVEVFGLETFLPGSQIDVKQITDYDSYVGKKMEFKVVKINEAI
                     KNAVVSHKALIESDIESQRQEIISKLEKGQVLEGTIKNITDFGAFIDLGGVDGLLYIT
                     DISWGRINHPNEVLELNQKLNVVVLDFDDNKKRISLGLKQLTPHPWDVLDASIDAGSK
                     VTGKIVNIEDYGAFLEISPGIEGLIHVSEVSWSNQPINSKEFFKLNDTYEAMVVTIDR
                     EDHKMSLSLKQLSADPWTEVNEKYSIGSRHSGLVKNLTPYGVFVELDENIGGMVHVSD
                     LSWVKRFNHPSEFTKVGENLEVVVLDIDNENRKISLGHKQIEEDPWDTFETVFPVGSV
                     HEATIIRKDDKGAVVSLPYGVEGYAPNRHLAKEDGSSAQLDETLSFKVIEFDRNDKRI
                     LVSHARFLNDKMADDRKDRTEQRKKDEKKGKTALKNVQKNIEKTTLGELDVLSQLKEK
                     MAADEQTAAKSTKATKAKAAKEEAPEAEENAPEAEEGGDEA"
     gene            2649..3056
                     /locus_tag="PK742_07600"
     CDS             2649..3056
                     /locus_tag="PK742_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQU76494.1"
                     /translation="MQYWLIKSDPETYGWKEMVRDGKTSWDGIRNYAARLHLRSMKKG
                     DKCLFYQSVKQPEVVGVVEVVKTAYPDPTAKDGDWSAVDVKVVAALPRPVTLADIKAE
                     NSLKDMVLVNNTRLSTQPVTEFQFNYILDMSRR"
     gene            complement(3059..3817)
                     /locus_tag="PK742_07605"
     CDS             complement(3059..3817)
                     /locus_tag="PK742_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQU76495.1"
                     /translation="MNKRYSVLIVEDEPIAADYLQKLIAEHCPDMQVIGQTDSVTETV
                     AWLRANHPDLIFMDIHLADDLSFRIFEECDVPAPVIFTTAFDHYAVKAFRVNSMDYLL
                     KPIDKKEFLEATEKFRKASLSNGTVESIMQAIRPPGQEYRERFLVQQGTELRSIAISD
                     TAYFYAEDKVVFLVATNGKKYILDNSLDKLESALDPEQFFRINRKILLSVNSIDSMQS
                     HSKSRVKIRLQPDLGLEAVVSVERSSDFKKWLDR"
     gene            complement(3810..4847)
                     /locus_tag="PK742_07610"
     CDS             complement(3810..4847)
                     /locus_tag="PK742_07610"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU76496.1"
                     /translation="MQLHTFKNRSILKWIVGVSLVPPTFFFLLNLLDPEASAEEKNIS
                     SYLIGFLVSFAITASLFYYNTWVLDATFVRWPKPAQQRKRLAVALTATILGSNMLALL
                     ISLLFNMWSGSPVELFSNVVAVTVITLVVSLWFLGYHFIELWRHSVLEKEQMEKEQIS
                     NQLAGLRHQLSPHFLFNSFNALQSLVEEDPQKAQQFVQQLSGMYRYTLVNAEEPLVPL
                     SDELHFIEKYLYLQQVRFGRNLKSTIDAEPGCMNMLLPSLSLQILVENAIKHNIVSAS
                     RPLHISILASGNQIQVINELQPRADTGTNTGIGLENLFKRYQLLNAGEPVIRRDDGKF
                     TAILPLLTTHE"
     gene            complement(4855..7233)
                     /locus_tag="PK742_07615"
     CDS             complement(4855..7233)
                     /locus_tag="PK742_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76497.1"
                     /translation="MKNSVVLLLLAMAFSAVSAQVPTQRIRGQVTDKQTTATLPGVNI
                     LVENSDPLIGSASDPDGYFVLENVPVGRVSLTLTYVGYEPVFLNNLELTSGKELLLNV
                     EMTEQVVTVEEVVIKASRDKTGALNEMATVSTREFTIEESMRYAGARNDVSRMAQNFA
                     GVRGGNDAVNDIVIRGNSPVGLLWRLEGVDIPNPNHFGDFGSTGGPVSMLNNNVLSNS
                     DFFTGAFPADYGNAVSGVFDLRMRSGNYEHHEFLGQIGFNGFEFGAEGPIHRDSKSTY
                     LINYRYSTLGVMSALGFDFGTGAAIPYYQDIAMKLDFPKTPAGKISVFALGGISQIDL
                     LASDETDTVDNLYNNELDIYDRSKLAVAGLNHTYLINNNSYTRLTLAASTLINKDVVD
                     SLSAETGDPVDFYRQNFQNSKLTASFFYKVKFNPKNNLQAGVRYEYILADLADSIIRQ
                     PEGVFIPLTSFDGNTFLLQPYVNWQHRFTDRITLNTGLHWQYLGLNGSNALEPRAGLR
                     YQVTEKDAFSVGYGLHSLMPPIAVFFRTVETSPGVFEQPNEDAGFMRSHHFVAGYDRL
                     FTENLRLKAEVYYQKLFDVLVDADSSSFSTVNYGSLLSGFPDQLANTGTGNNYGMEIT
                     FEQFLHRGFYFLITTSLYNSTYVGSDGIERQTAFDGDYVINALVGKEFRLPSGKESGR
                     PKYLVVDTRVTAAGGQRYTPIDIEATLLEGETVYNWAEAYTERFDDYFRWDGRVAFKM
                     EGRSTSQEWAVDVQNITNKSNPYGQSFNFETGETETTYQLGIFPVMQYRITF"
BASE COUNT         1955 a         1734 c         1825 g         1881 t
ORIGIN      
        1 agggtcattc ctgcgaaagc aggaatctct tactgatcaa ttgaccaaca atttacagga
       61 atgacgtatc tctttggagg gttattgtca atctatgaaa tctttaaagt cttcatctga
      121 gtaccagtct tccgcaagat ccagccaggg cgggtttgat tcatctatca atgctgtctt
      181 ccagccacgt ttccattttt taagctgctt ctcccttatg attgcatttg acatatccga
      241 atatccttca aaatagatca atctgtcaca ttcataccgg gcagcaaacc catccggatg
      301 cagtctgacc ttgtgctcaa agacccgtct ttccaaagaa tttgtaacac ctatgtacaa
      361 tacagacctt gttttattgg tcaggatata aacgtatgca ttgaactccc ggaactccat
      421 accagctttt cagcgctaaa atggccattt tcaggtagtt gcaatgagat tttattaacc
      481 gaaaatcagt aactttgcac tcccttgcgc ccgaaaacgg gtacaggcgc cggggtaccg
      541 cttgcggtaa ggctgaaccc ggatcattca cgtttaaaaa ttgtcctttg acaacagaga
      601 acgaaaacat ggagttggag aacaactccg aaactactgt tacagaagaa gtaacagaaa
      661 cttctaccaa ggcagagacc tctgccaccg aatccaaaga tcaacctgct tttgatgact
      721 tcgactgggg tgtcgacaag cgtaaccgta ctagctacgc taccgacaag gccgaggcca
      781 tggaaaaaga attcgaagct accttccgca ccctgcagga cgacgagatc gttctgggta
      841 ccgttgtagg tatcaccgac agcgatgtgg tgatgaacgt tggctataag agtgatggtc
      901 tgattccccg cactgaattc cgcgacatcg aagacctgaa agtcggcatg gaaatagacg
      961 tatatgtcgt ttccaaggaa gacaccaaag gccacctggt cctgtctcgc aagaatgcca
     1021 agatgatgca ggcatgggat aagatcgttg atgcgtataa taacgaaacg gttgtaacag
     1081 gtaacatcgt cagcaagacc aaaggtggtc tgatcgtgga ggtgttcggt ctggaaacat
     1141 tcctgccggg ttcacagatc gacgtgaagc agataaccga ttatgattct tacgtaggta
     1201 agaaaatgga attcaaagtg gtcaagatca acgaggccat caagaatgct gtggtatctc
     1261 acaaagcgct catcgaaagc gatatcgaat ctcagcgtca ggagatcatc tccaaactgg
     1321 agaaaggtca ggtactggaa ggtaccatca agaacatcac agacttcggt gccttcatcg
     1381 atctgggtgg tgtggatggc ctgctgtaca tcaccgatat ttcctggggt cgtatcaacc
     1441 acccgaacga agtactggaa ctcaaccaga aactcaacgt ggtggtgctc gactttgatg
     1501 acaacaagaa gcgtatcagc ctcggtctga agcagctgac cccgcatccg tgggatgtac
     1561 tggatgcttc catagacgct ggcagcaaag tgaccggtaa gatcgtgaac attgaagact
     1621 acggtgcctt cctggagatc tctcccggta ttgaaggtct gatccacgtg tctgaagtaa
     1681 gctggagcaa ccagcctatc aacagcaagg aattcttcaa gctgaatgat acttatgaag
     1741 ccatggttgt gaccatcgac cgtgaggatc ataaaatgtc cttgtctctg aagcagctga
     1801 gtgctgatcc gtggaccgag gtcaatgaga agtattccat cggcagtcgc cactccggtc
     1861 tggtgaagaa cctgaccccg tatggtgtat tcgtcgaact ggacgagaac atcggcggta
     1921 tggtacacgt atctgacctc agctgggtga agcgtttcaa ccatccttcc gaattcacca
     1981 aagtaggtga gaacctggaa gtggtagtgc tggatattga caatgagaac cgcaagatca
     2041 gcctcggtca caagcagatc gaagaggatc cgtgggatac tttcgaaaca gtattccctg
     2101 tgggctctgt gcatgaagcc accatcatcc gcaaggacga caaaggtgct gtggtatctc
     2161 tgccttacgg cgtggaagga tacgcaccca accgtcacct ggccaaggaa gacggcagct
     2221 cagcacaact ggatgagacc ctttctttca aggtcattga atttgaccgg aatgacaagc
     2281 gcatcctggt ttctcatgca cgcttcctga atgacaagat ggctgacgat cgcaaggatc
     2341 gcaccgagca gcgcaagaag gatgagaaaa aaggcaagac cgctctgaag aatgtgcaga
     2401 agaacattga gaagaccacc ctgggtgaac tcgatgtact gagccagctg aaagagaaga
     2461 tggctgctga tgagcagacc gctgccaaga gcaccaaagc caccaaggcc aaagcggcca
     2521 aggaggaagc acccgaagca gaagaaaatg ctccggaagc agaagaaggc ggcgacgaag
     2581 cctgatcagg ttagtcctta agtttcatat gaaccacccc attcggggtg gttctttatt
     2641 tttgggacat gcaatactgg ttgatcaagt ctgacccgga aacatacggg tggaaagaaa
     2701 tggtgcggga tggcaaaaca tcctgggatg gcattcgcaa ctatgcagca cgcctgcacc
     2761 ttcgttctat gaaaaaaggt gataaatgcc tgttctatca aagcgtgaaa caaccggagg
     2821 tggttggtgt ggtggaggtg gtgaagaccg cctatccgga cccgacagct aaagacggcg
     2881 actggagcgc agtggatgta aaagtggtgg ctgcattgcc acggccggta accctggccg
     2941 atatcaaagc ggaaaactcc ttgaaggata tggtactggt caacaatacc cgtttgtcaa
     3001 cccagccggt tacagagttt cagttcaact atattctgga tatgagcagg cgctgacctt
     3061 atctgtccag ccactttttg aaatctgacg atcgctctac actgactacg gcttcgaggc
     3121 caaggtctgg ttgcagacgg atcttcaccc tgcttttaga gtgcgactgc atgctgtcaa
     3181 tgctattaac agacagcagg atcttcctgt tgatcctgaa gaattgttcc ggatccagcg
     3241 ctgattccag tttatccaga ctattgtcaa ggatgtattt tttgccattc gtcgccacca
     3301 gaaagaccac tttatcttcc gcatagaaat aggctgtatc agatatggca atggacctga
     3361 gttcagtgcc ctgctgtacc agaaatcttt ctcggtattc ctgaccaggc ggacggatag
     3421 cctgcatgat ggattctacg gtaccattgg acagtgaagc ttttctgaat ttttctgtcg
     3481 cttcaagaaa ttcctttttg tcgatgggtt taagaagata atccatgctg ttcaccctga
     3541 aagccttcac agcataatga tcaaatgcgg tagtaaatat gaccggtgcc ggtacatcac
     3601 attcttcgaa gatgcgaaag ctgagatcat cagccaggtg gatatccatg aatatcagat
     3661 caggatgatt ggcccgtagc caggcgaccg tttcagtaac agaatctgtc tgtccgatga
     3721 cctgcatgtc aggacaatgc tctgcgatca gtttttgcaa ataatcagca gcaatgggtt
     3781 cgtcttcaac gatcaggacg ctgtatcttt tattcatgag ttgtcagtag tggaagtatg
     3841 gcagtgaatt tcccgtcatc tcttctgata acaggttcac cggcattgag caactgatat
     3901 cttttaaaca gattctccag gccaatgccg gtatttgttc ctgtgtctgc cctgggctgc
     3961 agctcattga tcacctgtat ctggttgccg cttgccagga tgctgatatg cagaggtctg
     4021 gatgcagaaa cgatgttgtg cttgatggca ttttctacca ggatctgcaa tgataatgat
     4081 ggcagcaaca tgttcataca cccgggttcg gcatcaatgg tgcttttcag attcctgcca
     4141 aacctgactt gttgcagata cagatatttt tcaatgaaat gcagttcatc tgataaggga
     4201 accagaggtt cttctgcatt gaccagggta tatctgtaca tgcctgatag ctgttgtacg
     4261 aattgctgtg ctttttgcgg gtcttcttct accagtgatt ggagtgcatt gaaactgttg
     4321 aacaggaaat gtgggcttag ctgatgtcgc aatccggcca gctggttact gatctgttct
     4381 ttctccatct gctctttctc caatacactg tgtcgccaga gttcaatgaa atggtatcca
     4441 agaaaccaca gactaactac cagggtaatg accgtaacgg ccactacatt gctgaacagt
     4501 tctaccggac tgccagacca catattgaac aggaggctga tgagcagtgc cagcatattg
     4561 gatccaagaa tagtggcagt aagtgctact gcaagtcttt tcctttgctg tgcaggtttg
     4621 ggccatctga cgaaagtggc atcaagtacc caggtattgt agtaaaataa ggaagcagtt
     4681 atagcaaaag ataccagaaa tccgatcagg tagctgctga tgttcttctc ttcggctgag
     4741 gcttccggat ccagcagatt cagcaggaaa aagaacgtgg ggggaaccag gctcaccccc
     4801 acgatccatt tcaggatgga cctgttcttg aaggtatgca gctgcataat ttggtcagaa
     4861 ggttattctg tattgcatga ccggaaagat acctaactga taggtggttt ctgtttctcc
     4921 tgtttcaaag ttaaaggact ggccgtaagg attgcttttg ttggtgatgt tctgcacatc
     4981 cacagcccat tcctgagatg tagaacgtcc ttccattttg aatgccaccc gtccatccca
     5041 tcggaagtag tcatcaaatc tttccgtgta agcttcagcc cagttgtaaa ctgtttcacc
     5101 ttccagaaga gtagcctcta tatcaattgg agtatagcgc tgtccgccag ctgctgtgac
     5161 cctggtgtcc accacgaggt atttcggcct gccactttct tttccagacg gcaacctgaa
     5221 ttcttttcca accagtgcat tgatgacata atctccgtca aaggcggtct gtctctcaat
     5281 gccatcgctg cccacataag ttgaattata caaagatgtg gtgatcagaa aatagaaacc
     5341 acggtggaga aattgttcaa aggtgatctc cataccgtag ttgtttccgg tacctgtgtt
     5401 ggcgagctga tcggggaaac cggacaataa tgaaccgtaa ttgacagtag agaaggaaga
     5461 gctgtcagca tctaccagca catcaaagag cttctggtaa tagacctctg ctttcaggcg
     5521 aaggttttct gtgaacaatc tgtcatatcc ggccacaaaa tggtgagagc gcatgaaacc
     5581 tgcatcttca ttgggttgtt caaatacccc cgggctggtt tccacggtcc ggaagaacac
     5641 tgcaatagga ggcatgaggc tatgcagacc atacccgacg ctgaatgcat ctttctcggt
     5701 tacctgatac cgcaaaccgg cacgtggttc cagtgcattg ctgccgttca gtcccaggta
     5761 ttgccagtgt aatccggtgt tcagcgtgat cctgtcagtg aatctgtgct gccagtttac
     5821 gtatggttgc agcaggaaag tattgccatc aaatgaggta agcggaatga aaacaccttc
     5881 cggttgcctg atgatgctgt cggccagatc tgcgagtatg tattcatatc tgacgccagc
     5941 ctgcaggttg tttttcggat tgaatttcac tttgtagaag aagcttgcgg tcagtttgct
     6001 gttctggaaa ttctgccgat aaaaatcaac aggatcgccg gtttctgccg ataaagaatc
     6061 cactacatct ttgttgatga gggtggaggc ggccagagtc aggcgtgtgt aggagttgtt
     6121 gttgatcagg taggtgtggt tcaatccggc cactgcaagt ttgctcctgt cataaatatc
     6181 aagttcatta ttatataagt tatcaactgt atctgtttca tcgctggcca gcaggtctat
     6241 ctgactgatt ccacccaaag caaaaacgga gatcttgccc gccggcgttt ttgggaagtc
     6301 cagtttcatg gctatatcct ggtagtaggg tatagcggca ccggttccga aatcgaatcc
     6361 aagggcactc atgacgccca gtgtggagta cctgtagttg atgaggtaag ttgatttgga
     6421 atcgcggtgg atggggcctt cagcaccaaa ctcaaagcca ttgaatccta tttgccccag
     6481 aaactcatgg tgctcataat ttccgcttcg catccgaagg tcgaatacac cactaaccgc
     6541 attgccatag tctgccggaa aggcacctgt aaaaaagtca ctgttgctca gcacgttgtt
     6601 attcagcata ctaaccggtc cgccggtaga accaaaatcg ccgaagtgat tggggttggg
     6661 tatgtccaca ccttccagtc gccagagcag accaacagga gagttccccc tgatcacgat
     6721 gtcattcact gcgtcattac cccctctcac accggcaaag ttctgcgcca ttctgctcac
     6781 atcatttcgt gcaccggcat agcgcatgct ttcttctatg gtgaattcac gggtgctgac
     6841 agtggccatt tcattcagag ctccggtctt gtctctggaa gctttgatga ccacttcctc
     6901 aacggtgacc acctgttctg tcatctccac attcagcaac aattccttgc cgctggtcag
     6961 ctccagatta ttcaggaata ctggttcata gccaacatat gtgagggtaa ggctgaccct
     7021 tcccaccgga acattttcca gtacaaaata gccgtccggg tcggaggcag agccaataag
     7081 cggatcactg ttttctacca gtatgttgac acccggcagg gtagcggtag tttgtttgtc
     7141 agtaacctgt ccgcggatgc gctgtgtagg tacttgcgca gaaactgcag aaaaagccat
     7201 cgccagcagg agtaaaacaa cagaattctt catggttttg gttttgatag agcaaaggaa
     7261 cagaacggtc acatgggtaa agaatatgtc tgacgaaccg gaacatacag gcgatgaacc
     7321 ggaagaaata catcatccta taggcagggt gatccggaag gtcgtgcctt tggcgttgga
     7381 ctgtttcaca aagat
//
LOCUS       DAOMAX010000123         7315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1541_length_7315_cov_3.923554, whole genome shotgun sequence.
ACCESSION   DAOMAX010000123 DAOMAX010000000
VERSION     DAOMAX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_7315_cov_3.923554"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..493)
                     /gene="secG"
                     /locus_tag="PK742_07620"
     CDS             complement(122..493)
                     /gene="secG"
                     /locus_tag="PK742_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQU76498.1"
                     /translation="MFTVILIIALVICVILALAVLIQNPKGGGLSGTFGGTANQLFGY
                     KRTSDDIEKITWYLGIALLVLCLGSAAFKPGTTGAQATPFEGQAVQPEQNFTPAPGGD
                     GATLDDTNTGDQAGSDDELPE"
     gene            complement(495..1289)
                     /locus_tag="PK742_07625"
     CDS             complement(495..1289)
                     /locus_tag="PK742_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76499.1"
                     /translation="MSVLQEWTEQYPWYALPWVLLAKKQHHEQDPDYPETLARAAMLV
                     PDREQLFRLIERTPLQEWLEEVIEVDTVTERTELTEKRQEEIVLNEPASLPQEEPVFE
                     YVPYEIEMQQAIRELELTGQAATLPDLPSLDDFQLTDGDTDTQLHRFTDWLTLVDGEE
                     RIGLVNRSAARRPIVLKPGKPVREEKKQASMPDSDDDFDEWQARELARRSSELSKEVL
                     SETLAAIMVSQGKTERAIFIYEKLGLKYPEKMAYFAGLIEDLKNEH"
     gene            complement(1352..1846)
                     /locus_tag="PK742_07630"
     CDS             complement(1352..1846)
                     /locus_tag="PK742_07630"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HQU76500.1"
                     /translation="MRTVTHLSLIMILLFSGCYTFNGNRIPDEIESVTVNFFANQSTF
                     VAPSLSQSFTEALKDKMAAETRLYLKDRGGDWEFSGAIVTYQNTPMAPTGNETTALNR
                     LTIGVKVDFVNHKNEQESWNQTFTRFEDYESSKSLAEVEGELIRLITIQLVDDIYLKA
                     ASDW"
     gene            complement(1843..3075)
                     /locus_tag="PK742_07635"
     CDS             complement(1843..3075)
                     /locus_tag="PK742_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQU76501.1"
                     /translation="MADEIRSIKQRFGIIGNAPGLNYALSIAARVAPTNLTVLITGES
                     GVGKEIFSQVIHQNSPRKHGPFIAVNCGAIPEGTIDSELFGHVKGSFTNAVDDRKGYF
                     ETVNGGTIFLDEIGEMPLGTQARLLRVLETGEFIRVGSSKVQKTDVRVVAATNVDLHQ
                     LIQTGRFREDLFYRLNTVPIRVPALRERHDDIYLLFRKFSSDFAEKYRTPLVQLDDAA
                     VQLLSSYNWPGNIRELKNVAEQVSVLSGQQTVSAGDLRAFLPDVQRTSLPMLRQEAEQ
                     HSASFTEREIFYKVLYDMKKDLNDLKQFVLTMAEANELKTDSGAVSGLMEERPVAALL
                     PMEHKNQQAPVVIEQGSKTTYPIEQQVEENLSLADKEKELIIKALKKHKGKRRDAALD
                     LGISERTLYRKIKEYELE"
     gene            complement(3082..4533)
                     /gene="miaB"
                     /locus_tag="PK742_07640"
     CDS             complement(3082..4533)
                     /gene="miaB"
                     /locus_tag="PK742_07640"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQU76502.1"
                     /translation="MYDQNPTLDQQESLLPDDRQGEVLTREEMEQAAAGRLFYLESYG
                     CQMNFSDSEIVASILHQHGFGLTRQFQEADLILLNTCAIRDNAEQRVRNRLQELRTVK
                     KQKPEALIGMLGCMAERLKARLLEEEQLVDIVCGPDAYRDLPQLVASADSGQKAVNVL
                     LSREETYSDIAPLRLDANGVTGFISIMRGCDNMCSFCVVPFTRGRERSRNAFTIVAEA
                     QDLFNRGYREVTLLGQNVDSYKWQHPETDNWYHFADLLKLVAEVSPLLRVRFSTSHPK
                     DITDEVLHTMAAYENICNYIHLPVQSGNSRILEKMNRTYDRDWYMERIDAIRRIVPDC
                     GLSTDIIAGFCSETEEEHQDTLSLMEQVQFDMSYMFFYSERPGTLAARKYPDDVPEDV
                     KKRRLNEIIALQNIHSANRYKQDIGKTFKVLVEKTSRRSAEDMCGRTDQNKMVVFPKE
                     NAAAGDYVFVKITDATSATLIGHMTEPVVAAVK"
     gene            4944..6035
                     /locus_tag="PK742_07645"
     CDS             4944..6035
                     /locus_tag="PK742_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76503.1"
                     /translation="MVWASLLWFANPTWFTHMASVSTELFLVACTLLALEAILSGKNR
                     LLALAIAVLLLISLRGMVIVATVWITYLLLEKPGSLRQFVRLTGPFLVGVLPFIAWNL
                     YHWQHTGWMLSHPDSPWIEHRALLSGKALIWSSGIFVFRFMEFGLCVICGILFRYRRW
                     FKENCRPIYWWTTTGVALLALASIPFNNPLTNRYLLFITVPLVIPFAAILSTLNRPLI
                     WLGVTCLLLASAHFYVYPEKWSKRVGYAYDATMAYRPYSMQLRTQMAQYLDEDAGISG
                     STVIWSGFPEYHPLKWTDPEATQRIYQHQMDTLHPDQADLIILSNIMNEVPYFTASRI
                     RQTWTPVATFRKGLIQLECYRNPKAVKNG"
     gene            6097..>7315
                     /gene="lepB"
                     /locus_tag="PK742_07650"
     CDS             6097..>7315
                     /gene="lepB"
                     /locus_tag="PK742_07650"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQU76504.1"
                     /translation="MEPVLPLAMTLYLIVSVVLLYAGLWKMFEKAGHAGWKAIVPFLN
                     GWICVKLIGKPVWRFLLLFVPIINAVMLFLIASELVRAYGKDGFWAGVAGMMFPQFYF
                     PYLGFNKEVRYLGIPAEVYKGQRKSIGREWADAIAFAVIAATFVRTFIFEAYTIPTTS
                     MEGELLAGDFLFVSKVNYGPRFPITPVAFPFAHHTMPLVGGKAYSEIIQLPYMRFPGL
                     QQLKRNDIVVFNFPEGDTIFKPIADQDYYDMKRANPNGFVPRPDQIGTRPLDKKENYI
                     KRCVGVPGDSLSILNGVLYVNGEKAFKGEHQQKSYRIIFKPNLNVPQEFLTDQGINLM
                     DFEGRNLFQDNQIYMVVNMDDATAAKLEKNPSVERVEPIYKGKMKTPDNPDYLPLRYY
                     PHDPELQLNSIDEF"
BASE COUNT         1726 a         1850 c         1694 g         2045 t
ORIGIN      
        1 gatgctggaa accatcatca gtgaatacga gcagaagcac aatattccgc cccccacccc
       61 tttcaaataa aaaaaaagca cccctcagcg ggatgccttt tcatgattgt tgatctattt
      121 cttattcagg tagttcatcg tctgaaccgg cctgatcacc tgtattggta tcatccagcg
      181 tggcaccgtc tccacccggt gcaggcgtga agttttgctc tggctgaacg gcctgccctt
      241 caaagggtgt agcctgcgct ccggttgtac ccggcttgaa cgcagcagag cccaggcaaa
      301 gcacaagcag tgctataccc agataccagg tgatcttttc aatatcatcg ctggttcgct
      361 tatagccgaa cagttggttg gccgttccgc caaaagtgcc gctcaaacct ccgcctttcg
      421 ggttttggat cagaactgcc agagccagaa tgacgcaaat gaccagggct attatcagta
      481 ttacagtgaa catatcagtg ttcgttcttt aagtcttcaa taaggcctgc aaagtaagcc
      541 attttttccg ggtatttcaa acccagtttt tcatagataa aaatggctct ttctgttttt
      601 ccctggctaa ccatgatggc agcaagggtt tctgaaagca cctctttgct aagctctgag
      661 cttctgcgag ccagttctct ggcctgccat tcatcaaaat cgtcatctga gtcaggcatg
      721 gaggcctgtt tcttctcttc cctgacaggc ttaccgggtt tgagtacaat agggcgccgg
      781 gctgctgaac ggttgaccag gccaatgcgc tcttccccat caaccagggt aagccagtct
      841 gtaaatctgt gcagttgcgt gtcggtatca ccatcagtca attgaaaatc atctaatgat
      901 ggcagatcag gaagtgtagc cgcctgaccc gtaagttcga gttctctgat agcctgctgc
      961 atttcgatct catagggaac atattcaaaa acgggttctt cctgaggcaa tgaagctggt
     1021 tcgttcaaga ctatttcttc ctgtcttttc tccgtcaatt cagtcctttc tgtcactgtg
     1081 tccacttcaa tgacctcttc cagccattcc tggagaggtg tgcgctcaat aagccggaac
     1141 agctgctctc tgtctggaac aagcatagct gcccttgcca gagtttccgg gtaatcagga
     1201 tcctgttcat ggtgctgctt ttttgccagg agcacccatg gcagagcata ccacggatat
     1261 tgttctgtcc attcctgtaa gaccgacatg tctgtacggg gtaataaatg tatatcttct
     1321 gctaaatgct gtagctgcat ggtctccatg atcaccagtc tgaggcggcc ttcaaatata
     1381 tatcatccac aagctggatg gtaatgagcc tgatcagttc accttctacc tcagccaaag
     1441 acttactgct ttcataatct tcaaaacggg taaatgtctg attccagctt tcctgttcat
     1501 tcttatgatt cacaaaatcg actttgacgc caatggtcaa cctgttcagt gcggtagttt
     1561 cgtttccggt tggagccata ggagtgtttt ggtaggttac aatggctccg ctgaattccc
     1621 agtcgccacc acgatctttg agatataatc tggtttctgc cgccatcttg tctttgagag
     1681 cctctgtaaa tgactggctc agcgaaggtg ccacaaacgt actctggttg gcaaagaaat
     1741 tgactgtaac actttcaatt tcatcaggta tgcggtttcc attgaaagtg tagcaacccg
     1801 aaaacaggag gatcattatc agcgacaggt gggttaccgt tctcattcca gttcgtattc
     1861 tttgatcttt ctgtataatg tccgttctga gatacccaga tcaagcgcag catcacgtct
     1921 ttttccctta tgcttcttta gtgccttgat gatcagttct ttctctttat cggccagcga
     1981 caggttttct tcaacctgct gctctatcgg ataggttgtt ttactgcctt gttcaataac
     2041 gacaggtgcc tgctggttct tatgttccat cgggagaagg gcagcaacag gcctttcttc
     2101 catcaaaccg gacacagcgc cggaatctgt tttcagttca ttggcctctg ccattgtaag
     2161 cacaaactgt ttcagatcat tgaggtcttt cttcatatca tacaagacct tgtagaagat
     2221 ctctctttct gtgaatgaag cgctgtgctg ctctgcttcc tgtctgagca tgggcaaact
     2281 agttctttgc acatcaggca ggaaagccct caggtcacct gcagaaacag tttgctggcc
     2341 agaaagcaca gaaacctgtt ctgccacatt cttcagttca cgaatattac caggccagtt
     2401 ataggatgat aggagctgaa ccgcagcgtc atccaactgg acgagcggtg tacggtattt
     2461 ttcagcaaaa tctgaactga actttcggaa aaggaggtag atatcatcat gccgttcacg
     2521 cagggcaggc actcttattg gaactgtatt gagcctgtag aacaggtctt cgcggaacct
     2581 tcctgtctga atgagctgat gaagatctac attggtggca gccacaacac gcacatcggt
     2641 tttctgcact ttggatgagc ctactctgat gaactcgccg gtttccagaa cgcgaagcaa
     2701 gcgtgcctgg gtacccagcg gcatctcgcc gatctcatcc agaaaaatgg tgcctccgtt
     2761 cacagtctcg aaatagcctt tccggtcatc cacagcattg gtgaaagacc ctttcacgtg
     2821 gccgaacaat tctgaatcaa tggttccttc cggtatagcg ccgcagttga ccgcaatgaa
     2881 aggcccgtgt tttctcgggc tgttctgatg tatgacctgg ctgaagatct ctttccccac
     2941 accactttcg ccggtaatga gcaccgtcag gttggtaggt gccacccggg cagctatact
     3001 gagtgcatag ttcagacccg gtgcatttcc tataatgcca aaacgttgtt tgatgcttct
     3061 gatctcgtct gccatgaggc gttatttgac cgctgccact accggttcgg tcatatggcc
     3121 gatgagggtg gccgaagtgg catcagtgat ctttacaaat acataatctc ctgcggcggc
     3181 gttctccttt ggaaacacta ccatcttatt ctggtcggta cgaccgcaca tatcctctgc
     3241 tgatcttctg gaggtctttt ctacaagcac tttaaatgtc ttacctatat cttgtttata
     3301 gcggtttgca gaatggatgt tctgcaatgc gatgatctcg ttgagtcttc tttttttgac
     3361 gtcttcaggt acatcatccg gatactttcg ggcggccagc gtgccaggtc tttcactgta
     3421 aaaaaacata taagacatat cgaactgcac ctgttccata aggctcaggg tatcctgatg
     3481 ctcctcttca gtttctgagc agaaacccgc tatgatgtct gtactaagcc cgcagtcagg
     3541 aacgatacga cgaatggcat cgatcctttc catgtaccag tcgcggtcat aggtacggtt
     3601 cattttttcc aggatgcggg agttgcctga ttgcaccggc agatggatgt agttgcagat
     3661 gttctcatat gcggccatgg tatgcagtac ctcatctgtg atatccttag gatgagaggt
     3721 actgaagcgt acccgcagca gcggactgac ttctgccacc agcttcagca ggtcagcaaa
     3781 atgataccag ttgtctgttt ccgggtgctg ccacttgtaa ctatccacat tctggcccag
     3841 cagcgtcacc tcgcggtagc ctcggttgaa gaggtcttga gcttcagcta ctatggtgaa
     3901 ggcattgcgg cttctctccc gtccgcgggt aaagggtacc acacaaaaag agcacatatt
     3961 atcacaaccc cgcataatgg agatgaaccc ggtaacccca ttggcatcga gtctgagagg
     4021 tgcaatatca gaataggtct cttctctgga gagcagcaca ttcactgctt tctgtccgct
     4081 gtcagcactg gccaccagtt gtggcaggtc acggtaagca tccgggccac agacaatatc
     4141 caccaactgc tcttcctcca gcaaacgtgc tttcagtcgt tctgccatgc agcccagcat
     4201 tccgatgagg gcttccggtt tttgcttttt gaccgtgcgc agctcctgca ggcgatttcg
     4261 taccctttgc tccgcattat cgcggatggc acaggtattc agcaggatca gatcggcttc
     4321 ctggaactga cgtgtcaggc caaaaccgtg ctggtggagg atggaagcca cgatctcgct
     4381 gtcactaaag ttcatctggc aaccataact ttccaggtag aacagacggc cggcagctgc
     4441 ctgttccatc tcctccctgg tcaacacctc accttgtcgg tcatccggca gaagagattc
     4501 ctgctggtca agtgttggat tctgatcata catggtgcaa atttacaaag aatgactgtc
     4561 aatttgacag caaaacacca gtatctttac cctgcaacat ggatacccgt aacgatcaga
     4621 aatatttaca catttcttgg ctgatcctgt tttcaggttc cgttttgtta accctgctcc
     4681 atttgcacct gccgctgttt tcagaccagg aaaccatcat cggttttccg gctatctatt
     4741 tctatgagca ccctggcagc ggcatgctac ttccggtcac tcaagataca ggacacccac
     4801 cacttatacc actgtatttc agtttattat acagattatt tacaccttct attgagttat
     4861 tgcactgggc ctcactgccc tttctgttgc tgtccggata cttgtttcac aagctctgcc
     4921 aaaggtggct ttctggtcct gctatggtct gggcatcgtt gctgtggttt gccaatccca
     4981 cctggtttac ccatatggca tcagtcagca ccgaactgtt tttggtagcc tgcacacttt
     5041 tggcacttga agctatactt tccggaaaaa accggctgct ggcactagcc attgccgtac
     5101 tgctgcttat cagtttgcgg gggatggtca tagtggcaac tgtctggatc acttatctgt
     5161 tactggagaa accgggaagc ctccggcagt ttgtcaggtt gacaggccca tttctggtcg
     5221 gcgtgctgcc cttcattgcc tggaatctct atcactggca acataccggc tggatgctca
     5281 gccaccccga ttcaccatgg atagaacacc gtgccttact atccgggaaa gcactgatct
     5341 ggtcatccgg aatatttgtt ttcaggttca tggagtttgg cttgtgcgtt atctgcggga
     5401 tattattccg ctacagacga tggttcaaag aaaactgcag gccgatatac tggtggacca
     5461 caacgggagt ggccttactg gcacttgcat ccattccgtt caacaacccg cttaccaaca
     5521 ggtatctgct ttttattacc gtgccgctgg tcattccgtt tgcagcaatc ctgtccactt
     5581 tgaacaggcc gcttatttgg ctgggcgtaa cctgtttgct gcttgcatca gctcattttt
     5641 atgtgtatcc tgaaaaatgg agtaagcggg ttggatatgc ctatgatgca accatggctt
     5701 accgcccgta cagcatgcag cttcgtaccc aaatggcaca gtatctggat gaagacgctg
     5761 gtatttccgg atcgactgtg atctggtctg gctttccgga ataccaccca ttaaaatgga
     5821 cagatcctga agctacccaa cgtatctatc agcaccagat ggataccctg catccagatc
     5881 aggcagatct catcatcctg tctaacatca tgaatgaggt tccctatttc acagcaagtc
     5941 gtatcaggca aacatggact cctgtggcca ctttcagaaa agggcttata cagctggaat
     6001 gttaccgtaa cccaaaggcg gttaaaaatg gttaatgcgg tcaaaactcc atacttttga
     6061 cgccattgcg ctgttgatta agcaatcaag aaaaagatgg aacctgtact acccctggct
     6121 atgacacttt accttatcgt gtctgtagtt ttgctttatg ccggattgtg gaaaatgttt
     6181 gaaaaggcag gacatgccgg atggaaggcc attgttccct ttctcaatgg atggatatgt
     6241 gtgaaactga taggcaaacc ggtttggcgg ttcctactgt tgttcgtgcc catcatcaat
     6301 gcagtcatgc tgttcctgat agccagtgag ctggttcgag cctatggcaa agacggattc
     6361 tgggcaggtg ttgcaggtat gatgtttccc cagttctact ttccttatct cggattcaat
     6421 aaagaggtca gatatcttgg aattccggct gaggtgtata aaggtcagcg gaaatctatt
     6481 ggcagagagt gggcagatgc aatagcattt gctgtaatag ctgccacttt tgtgcgcacc
     6541 ttcatcttcg aagcctacac catccccact acctccatgg aaggcgaact gctggctgga
     6601 gattttctgt tcgtcagcaa ggtcaattac ggtccgcggt ttcccatcac acccgtagct
     6661 ttcccgtttg cacaccatac catgcctctg gttggcggaa aggcctattc tgagatcata
     6721 cagttgcctt atatgcgatt tcccggactt cagcagctga agcggaatga cattgtcgtg
     6781 ttcaattttc cggaaggtga taccatcttc aaacccatag ctgatcagga ttactatgac
     6841 atgaagcggg ccaacccgaa tggttttgtg cccaggcctg atcagattgg caccagaccg
     6901 cttgacaaga aagagaatta tatcaaacgc tgtgtgggtg ttcccggaga ttcactgtcc
     6961 atccttaacg gagtgttata tgtgaatggc gaaaaggctt ttaaaggcga acatcagcag
     7021 aaatcttacc ggatcatctt taaaccgaac ctgaatgttc cgcaggagtt tctgaccgat
     7081 cagggcatca acctgatgga ctttgaagga agaaatttat tccaggacaa ccagatctat
     7141 atggtggtga acatggatga cgccactgcc gctaagctgg agaaaaatcc ttcggtagaa
     7201 agagttgagc cgatctacaa ggggaaaatg aagactcctg acaatccgga ttatttacca
     7261 ctgcggtatt accctcacga ccctgaacta cagttaaatt ctatagatga attcg
//
LOCUS       DAOMAX010000124         7243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1569_length_7243_cov_3.948664, whole genome shotgun sequence.
ACCESSION   DAOMAX010000124 DAOMAX010000000
VERSION     DAOMAX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_7243_cov_3.948664"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..3170)
                     /gene="secDF"
                     /locus_tag="PK742_07655"
     CDS             complement(129..3170)
                     /gene="secDF"
                     /locus_tag="PK742_07655"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HQU76505.1"
                     /translation="MQGKGLIQFFLVTLIIVCLYQLSFTWVASRVEKSASAYAESAVP
                     ADADPAQRSILVAEKRYEYLTEKANETVYNIGIAKYNYEEVKERAINLGLDLQGGMSV
                     MLEVSKYDLIKSLSNNPKNPKLIAALDAARTAEGKGQGDLIDLFVDAYEQQNPGSQLV
                     SLFNSRDNSDELAATATNAEVRSFLADEAQRGINSTYTKLKERIDKFGVSQPYITLQE
                     STGRIIVELPGVDNPKQVRKLLQSTANLQFWETYTIQELSSNLTSANEALRTALEGGV
                     DEIAEETPGDTTQAAENGEFFAQADTTAGDTTVESGLLNDTLLADEPLTDSTDAAAGP
                     LINKVLLPPGSGGPVIGYVNVADTALLMDYMHREEVLNAMPGDCKIMLASTPLPDNKD
                     LYAVYAMKTKLDGTGALLEGDVISKAYQDFDPTNGTPEVTMVMKSDGAKAWRRITSEN
                     VGSFIAITLDNQVYSAPRVNEEIPNGRSSISGSFDIKEAQILANILEVGKLPVPSRIV
                     QEDIVGPSLGAESIRVGLISLVIGLLMVVLFMIFYYSNAGVISDIALFANIFFIFGVL
                     ASLGATLTLPGIAGLVLTMGMAVDANVIIFERIREELAKGKTMVKAIADGYLHSYSAI
                     IDANITTLIIGFILSQIGTGPVKGFAVVLIIGILSSLFCSIFITRLILDRMSRKNKEV
                     KYYTSMTKNWFNNVNINFMGKRRIGYMFSAGLTIIGLVFMLSSGFDLGVDFKGGRTYT
                     VRFDQPVEAESVRTYLTNNFDNKIQVKSFSGNDKLKITTSYKIDETTPETDSLVAVAL
                     YQNLTSFYTGDVSFDTWDSSYKESQMKVEATVADDIQQSAGRALAVALLAIFVYIVLR
                     FRRWQFGLGALVSLVHDAFMVVALFSIFKNLMPFSMEINEAFIAALLTVIGYSINDTV
                     VVFDRIREYLRESRSGSIKDIFNAAINQTLSRTLITSGTTLLSIIVLLIFGNDNIQGF
                     AFALFIGIGFGTYSSIFVASALAVDFYRRSDRDKAADKVMK"
     gene            3317..4129
                     /locus_tag="PK742_07660"
     CDS             3317..4129
                     /locus_tag="PK742_07660"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76506.1"
                     /translation="MKKPGIWCLFILAGIVVNGQTATEWFNQAFEKYIEGDYTGAVED
                     YTQCIALAPEDASAYFNRGLSYYYMGKMQEAADDFDLVTKLSPGDAEGYYYKGYVLAE
                     LGRYLDALQALTSAIYYDANYLDAYMERGYVRMLLTQYDVAITDFNSAHQLDAENMEV
                     LFNRGLCLQTLEDFGAAEKDFREVVAVLPDMEDAWFNLAYCLLQLHEYTESAELFGQV
                     LLLNPDAGDAYYNRGLAHYYLYEDGAACDDWTRAQSMGVEIDQQQLQQVCGQ"
     gene            complement(4180..5094)
                     /locus_tag="PK742_07665"
     CDS             complement(4180..5094)
                     /locus_tag="PK742_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76507.1"
                     /translation="MKITLRNMLAVCLMAPLAVFAQALPNGDMESWEPLLLGGGEKPV
                     GWDTPDVIAGALGITDRVVEKDDASVHSGSFAARLTTKDLEAPAPIGTLTVPGTLALG
                     TIIFDPLTLETGVVGGLAMSEAPDAVIGYYTYAPASGDTMNVTVVALKAGETIGAGEF
                     RTSSSVASYTMFEAPITYFVSETPDTIQVIITSSGGFASATAGSVAHIDALAFTGISA
                     TEDLTAAGLGVTIFPNPTTDFIHVLNPSGNAATVELFNLNGQRTDVFQVNGGTTALDV
                     RHLSAGMYLMRMSQNGQLMYSGKFRVTQ"
     gene            5273..5743
                     /locus_tag="PK742_07670"
     CDS             5273..5743
                     /locus_tag="PK742_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQU76508.1"
                     /translation="MSVTVGQTAPSFTLYDTEKQQVSLESFRGRPVVLLFYPQAFTGT
                     CTAELCATRDDIGSYDKVQAQVLAISVDSVFTLKKFKDDQQFNFPLLSDFNKETSRAY
                     GALYEQWILDMQGVSKRSAFVIDAAGVIRYAEVLENPGELPDFEAIKSTLASLS"
     gene            5746..6183
                     /locus_tag="PK742_07675"
     CDS             5746..6183
                     /locus_tag="PK742_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQU76509.1"
                     /translation="MFNIRVYGLIFRSDHVLVSSEQVAGRQVLKFPGGGLEFGEGTLD
                     ALRREFLEETGYSISILGHLYTTDYFVRSFLSPEDQVISIYYRAELEGHSGLDTVAPV
                     LPDQTFHWIPWKSALPEDFTFPIDSEVLRRLQAGELFRPGRET"
     gene            complement(6161..>7243)
                     /locus_tag="PK742_07680"
     CDS             complement(6161..>7243)
                     /locus_tag="PK742_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQU76510.1"
                     /translation="VAYGRDVRSHPVGTGPFTFVKWLEGNMLILHRNPNYFERDDQGN
                     QLPYLDGVNIRFIANKSTEFLKFMNGELDFVSDIDVSLKNQILSADGQLRPEYADRFS
                     LLKGPYLNTEYLSILMDSDAAVLRGNPLQFKEVRQAINMAFSRQEMLLFLRSNRGIAA
                     TGGVVPPSLLGTESSVSYGYDYNPEKAARLLEEAGFAGGVGLPEMVLHTTAEYQDFAT
                     YLKDKLEDIGIALRIETVDPRVLREMRIKEETPFFRSSWIADYSDAESYLQMFFGGNG
                     APPNYTRYNNHAFDSLYMAAVTETIPAERIRLYRQMDSLMMQEAPIVPLYYDEVYRFV
                     GKDVEGLEPDALNHLRLKKVRFRDPA"
BASE COUNT         1786 a         1726 c         1856 g         1875 t
ORIGIN      
        1 aactgcacag cagcagaatg atcaggaatg ttcgcatgaa acgatgttgc agggtgagat
       61 atcccattgg ttaaagctaa aaaaaaacct cccctgaggg aggtttatca caaaatttat
      121 gccttgtatc atttcatcac cttgtctgca gctttgtcgc ggtcgcttct gcgatagaaa
      181 tcgactgcca gggcggaggc tacgaagatg gatgagtagg taccgaaacc aataccgatg
      241 aacagtgcaa aggcaaagcc ctgaatgtta tcgttgccga agatcagcag aacgatgata
      301 gacaggagcg tggtgcctga ggtgatcagg gtacggctca gggtctggtt gatggcagca
      361 ttgaagatgt ctttgatact gcctgaacgg ctttcacgaa ggtattccct gatccggtca
      421 aacaccacca cggtatcgtt gatggagtaa ccgatcacag tcagcaatgc cgctataaat
      481 gcttcgttga tctccatgga aaacggcatg aggttcttga atatggagaa aagtgctacc
      541 accatgaaag catcatgtac aagtgaaacc agtgcaccga ggccaaattg ccaacgtcgg
      601 aaacgcaaca cgatatagac gaagatggcc agcagcgcta cagccagagc tcttccggca
      661 gattgctgga tatcatcagc cacagtagct tccactttca tctggctttc tttgtaggag
      721 ctgtcccagg tatcaaagct tacatcacct gtatagaaac tggtgaggtt ctgatacagg
      781 gccaccgcaa ccaggctgtc tgtttcaggg gtggtttcat ctatcttata agaagtggtg
      841 atcttgagtt tgtcgtttcc gctgaaactc ttcacctgga tcttgttgtc aaaattgttg
      901 gtcagatagg tacgtacgga ttctgcttct accggctggt cgaatcggac agtgtaggta
      961 cgtccacctt taaagtccac acccagatcg aatccgctgc tcagcatgaa taccagacca
     1021 atgatggtga gaccggcaga gaacatgtaa ccgatacggc gtttgcccat gaagttgatg
     1081 ttcacattgt tgaaccagtt cttggtcatg ctggtatagt acttcacctc tttgttcttt
     1141 ctggacattc tgtccaggat cagacgggtg atgaagatgg aacagaacag ggatgacagg
     1201 ataccaatga tcagcacaac tgcaaatcct ttcaccggac cggttccgat ctgcgacagg
     1261 atgaaaccga tgatcagcgt ggtgatgttg gcatcgatga tggcagaata ggagtgcagg
     1321 taaccatcgg cgatggcttt taccatggtc ttacccttgg ccagttcttc ccggatccgt
     1381 tcgaatatga tgacgttggc atccacggcc atacccatgg tcagtaccag accggcaata
     1441 ccgggcaggg tcagggtcgc tccgagggag gccagtacgc cgaaaatgaa gaagatattg
     1501 gcaaacagtg cgatatcaga gatcacaccg gcattgctgt agtagaagat catgaacaag
     1561 accaccatca acagaccgat cacgagagag atcagaccta cgcggatgga ttcagcaccc
     1621 agtgaaggac ctacgatgtc ttcctgtacg atgcgggatg gaaccggcag cttacctact
     1681 tccaggatat tggcgaggat ctgtgcttcc ttgatatcaa agctgccgga gatggaagat
     1741 cgtccgttgg ggatctcttc attcacacgg ggagcagagt aaacctgatt gtcaagtgta
     1801 atggcaatga aactacccac attctcactg gtgatccttc tccacgcctt ggcaccgtca
     1861 gatttcatta ccatggtcac ctcaggtgta ccgttggtgg ggtcgaaatc ctggtatgct
     1921 ttgctgatca catctccttc cagcagggcg cctgtgccat ccagtttggt cttcatggca
     1981 tagacagcat acagatcctt gttgtccgga agtggcgtgc tcgccagcat gatcttacaa
     2041 tcgccgggca tggcattcag cacttcttcg cgatgcatat agtccatgag cagagctgta
     2101 tctgctacat tcacatatcc gatgaccggg ccaccgctac ccggaggcag cagcaccttg
     2161 ttgatgagag ggccggcggc agcatcagtg ctgtcggtca gaggttcatc tgccagcagg
     2221 gtgtcattca gcagaccgga ttctacagta gtatcaccgg cggtggtgtc tgcctgtgca
     2281 aagaattcac cattttcagc agcctgggtg gtatctcccg gagtctcttc tgcaatttca
     2341 tccacacccc cttccagagc ggtacgaagg gcttcattgg cactggtcag gttagaagat
     2401 aattcctgaa tggtataggt ttcccagaac tgcagattgg cagtactttg caacagtttg
     2461 cgaacctgct tggggttatc tacaccggga agttccacta tgatacgtcc ggtgctttcc
     2521 tgcagggtga tgtaaggctg tgatacaccg aacttgtcga tacgttcttt cagtttggta
     2581 taggtggagt tgatgccacg ctgggcttca tcagccagga aggaacgaac ttctgcattg
     2641 gttgccgtag ctgccagttc atctgagtta tcgcggctat tgaataatga cacgagttga
     2701 gagcccggat tctgctgctc ataggcatcc acaaacagat cgatcaggtc tccctgacct
     2761 ttcccttctg cggtgcgtgc tgcatccagt gcggctatga gtttaggatt cttggggttg
     2821 ttggacaagc tcttgatcag gtcatacttg gatacctcca gcatgacgct catgccgccc
     2881 tgcagatcca gacccaggtt gatggcccgt tcctttacct cttcgtaatt gtattttgcg
     2941 ataccgatgt tatacacggt ctcgttggcc ttctcagtca ggtattcata tctcttttct
     3001 gcaaccagaa tggaacgttg agccggatca gcatcagccg gaactgcaga ttctgcatag
     3061 gctgaagcac ttttctctac cctggatgct acccaggtga atgacaactg atacaggcac
     3121 acaatgatca gcgtgaccag aaagaactgg attaaacctt taccctgcat atactcttga
     3181 aattattgtt ggttttaagc ttgaaaaaca gcgtgcaaat ataggaaatc ttttgagcaa
     3241 gatgccgtta tcacaagggt ttggaaggag ccggtcaacg ggatttccag tacttttgac
     3301 ctgaaaacgt gataccgtga aaaaaccagg aatttggtgc ctgttcattc ttgccgggat
     3361 tgttgtgaac ggtcaaactg ctacagaatg gttcaatcag gcatttgaga agtacattga
     3421 gggcgattat acgggagcgg tggaagacta tacccagtgt attgcacttg cacctgaaga
     3481 tgcctcggcc tatttcaaca ggggtttgtc ttactattat atgggtaaaa tgcaggaggc
     3541 tgccgatgat ttcgatctgg taaccaaact gtcgcccgga gatgccgaag gttactacta
     3601 caaggggtat gttttagctg agctgggcag gtacctggat gccctgcagg ctctgaccag
     3661 cgccatctat tatgatgcga attacctcga tgcctatatg gaacgagggt atgtcaggat
     3721 gcttctcaca caatatgatg tggccattac agatttcaac agtgcacacc agctggatgc
     3781 agagaatatg gaagtgctgt tcaaccgcgg gctgtgtttg caaacactgg aagatttcgg
     3841 agcagcagaa aaagatttca gggaagtggt agctgtcctt cctgatatgg aggatgcctg
     3901 gttcaacctg gcctattgct tgctccagct gcatgaatac actgaatcag cagaattgtt
     3961 tggacaggtc ctgctgctga accctgatgc gggcgatgca tattacaaca gagggctggc
     4021 acattattac ctgtatgagg acggtgcagc gtgcgatgac tggacaaggg cgcagtctat
     4081 gggtgtggag atagaccagc agcaacttca gcaggtttgc ggtcaataaa aaaaggctcg
     4141 tcacagtgga cgagccttgt acaatttcaa gctttcagat cactgggtaa cgcggaattt
     4201 gccgctgtac atcagttgcc cgttctggct cattctcatc aggtacatgc ctgcagaaag
     4261 atggcgaaca tccagtgcag tagtaccgcc atttacctgg aatacatcag tgcgctgacc
     4321 gttcaggttg aacagttcaa ccgtagctgc attgccgctt ggattcagta catggatgaa
     4381 gtcagtggtc gggttcggga aaatggtcac gcccagaccg gcagcggtca ggtcttcagt
     4441 agcagagata ccggtaaagg ccagggcatc aatatgggct acactacctg ctgttgcaga
     4501 tgcaaaacca cctgaagagg tgatgatcac ctgaatagtg tctggtgttt ctgaaacaaa
     4561 ataggtgata ggtgcttcaa acatggtgta actggccaca ctggaactcg tgcggaattc
     4621 tcctgcgcca atggtctcac ctgctttcag agcaaccaca gtaacattca tggtatcgcc
     4681 tgatgcagga gcataggtgt agtagccgat tactgcatct ggtgcttcag acatggccag
     4741 acctccaact acacctgttt ccagtgtaag aggatcaaaa atgatggttc ccagagcaag
     4801 ggttcccggt actgtcaggg ttccgatagg agcgggtgct tccaggtctt tggtggtcag
     4861 tctggcggca aaagatcctg aatgtacaga ggcgtcatct ttttctacca ctctgtcagt
     4921 gattccaagt gcacctgcaa ttacgtcagg agtgtcccaa cccacaggtt tttcgcctcc
     4981 tcccaacagg agaggctccc agctttccat atcgccgtta ggcagtgcct gggcaaaaac
     5041 ggccaatgga gccataaggc aaacagccaa catgttgcgt agcgtaatct tcatacgatt
     5101 gattttattg ttttatccca caaatataat attctgttta cagccggatg catcagaaag
     5161 gttttttgca cctacaggct cagggttgta gtgaaccaat ggcgtatatc gttgtttcct
     5221 gaccataaag gcatgtatta cctttatgcc ctcattgtaa aaattatatc atatgtcagt
     5281 tactgtaggc cagacggctc cctcgttcac tttatatgac acagaaaaac agcaggtttc
     5341 actggaatct ttcaggggcc ggcctgttgt actgttattt taccctcagg catttaccgg
     5401 aacctgtact gctgaactct gcgctacccg cgatgatata ggcagttatg ataaggtgca
     5461 ggcacaggta ttggccattt ctgttgattc ggttttcacc ctgaagaaat tcaaggatga
     5521 ccagcagttc aacttcccct tattgtctga tttcaataag gaaacctcac gtgcctatgg
     5581 tgccttgtat gagcaatgga tcctggatat gcaaggtgta tctaaacggt cggctttcgt
     5641 gatcgatgcg gcaggtgtga tcaggtatgc agaggtgctc gaaaacccgg gtgaactgcc
     5701 tgatttcgaa gcgatcaagt ccactctggc atctttgtcc tgactatgtt caacatcagg
     5761 gtttatggtc tgatttttcg gtctgaccat gtgctggttt ccagtgaaca ggtggctggc
     5821 cggcaggtgc tgaagtttcc gggcggcgga cttgaatttg gtgaaggtac cctggatgca
     5881 ctgcgacggg agtttctgga agaaacagga tattcaatta gcatcttagg ccatctttat
     5941 acgaccgatt actttgtcag atcctttctg tcgcccgaag atcaggtcat cagcatttac
     6001 taccgggcag aactggaagg ccatagtggt ttagatacag tagcgccagt gcttcccgat
     6061 cagacatttc actggatccc ctggaagagc gccttaccgg aagatttcac gtttcctatt
     6121 gacagcgagg tgcttagacg cctgcaggcc ggcgagcttt tcaggccggg tcgcgaaacc
     6181 tgaccttctt aagccgaagg tggttcaggg catccggttc cagtccttcc acatccttgc
     6241 ctacaaagcg atatacctca tcataataca gcggaacgat gggagcttcc tgcatcataa
     6301 gactgtccat ctggcggtac aggcggatac gttcagcagg aatggtttct gtgactgcag
     6361 ccatgtataa gctgtcgaag gcgtggttgt tgtatcgggt atagttggga ggtgccccgt
     6421 ttccgccaaa gaacatctgc agataacttt cagcatctga ataatcggca atccaggatg
     6481 atctgaagaa gggcgtctct tcctttatac gcatttcccg cagtaccctg gggtcaaccg
     6541 tttctatgcg cagagcaata cctatatcct caagcttgtc tttcaggtaa gtagcaaaat
     6601 cctggtattc tgcagtggta tgcaacacca tttccggcag ccccacccca cctgcaaacc
     6661 ctgcctcttc cagcagtcgg gcagcttttt ccgggttata gtcatagccg taggagacag
     6721 aactttctgt acccagcagg gaaggaggaa caacaccgcc ggttgctgct atgcctctgt
     6781 tgctccggag aaaaagcagc atttcctgcc tgctgaatgc catgtttatg gcttgtctta
     6841 cttctttgaa ctgcagcggg tttccacgca atacggcagc gtcagaatcc attaatatgc
     6901 tcaggtactc cgtattcagg taggggccct tcaggagact gaaccggtct gcatattcgg
     6961 gtctaagctg gccgtcggcg ctcaggatct gattcttgag gctgacatct atatcactga
     7021 caaagtccag ttcgccgttc atgaatttga gaaactcggt tgatttgttg gcgatgaatc
     7081 tgatattgac gccatccaga tagggcagct gatttccctg atcatcccgc tcaaaatagt
     7141 ttggattacg gtgcaggatc agcatatttc cttccagcca tttgacaaat gtgaagggac
     7201 cggtgccaac cggatggctg cgaacatctc ttccatatgc aac
//
LOCUS       DAOMAX010000125         7198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1582_length_7198_cov_4.330393, whole genome shotgun sequence.
ACCESSION   DAOMAX010000125 DAOMAX010000000
VERSION     DAOMAX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1582_length_7198_cov_4.330393"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2493
                     /gene="sprA"
                     /locus_tag="PK742_07685"
     CDS             <1..2493
                     /gene="sprA"
                     /locus_tag="PK742_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HQU76511.1"
                     /translation="FPSDLGIRLPLYASISESFSNPQYDPYTFDIDLKDKVNDIEVLY
                     GADSAKAYQRQAIEYVSIKSINLTNIRIDRPKSSGTPKIYSIQNFDATLAYSQDYNSS
                     PVLEHDIIDRYKASLGYNYAIRAKSVKPFEKMVDKKDAWLALVRDFNFNLLPTSYSFR
                     TDMNRQFGETLMRDIYGDGLIQPTFNKFFNWDRFYGLKLEPFTAMKIDFSATTNTRID
                     EPYGRIDTPEEKDTIWNNIRDLGRTINYRHSVSASYNVPLNKIPALSWTSIRTNYNIT
                     YGWTAGSLGLADSLGHILTNTQAKTINGELNFRNLYNKSPFLKQYNNNTFSLKGDKGG
                     RGGKDELNAADAEDDKDKKKKIKKVSPTAEVMVRLLLSLKRVTINYTENYSTTLPGFM
                     LTPDLMGLDLKDGPAPGWDFVMGIQPSGNWLDSAASKGWISSAGTFNSTFLQNYSRNL
                     DVRTNLEPVPDLRLDINLNKSYSENHSEFFRNIGTLDEVDFAHLNPMDMGSLTMSYSI
                     LGTIFDKLNTTDVTETFRQFEANRVIISERFGNENIYSDGQFFDPQDSTFLENYFDGY
                     GPYSQDVLVPSFIAAYRDIDAATIPLNVFEQMPRPNWRLTYTGLTKIDAMKKIFSNFT
                     INSAYTSTLAVNSYMTNLNYFGNYYLYANTVDTLSGNYYSLFSIPNVVLSEQFSPLFG
                     VDATFVSSLSTKFDFRKTRTLNMSFVDYQLTEIRSTEVTVGLGYTTSGITLPIKIKGK
                     RPTLENDVTFRFDMSYRDDITTNFRLDQNLNEPTGGLTVIQISPTIDYLVNDRFNVQL
                     YFDRQQTIPKISTAYPITTTNAGVKVRMSLAE"
     gene            2545..2925
                     /gene="gcvH"
                     /locus_tag="PK742_07690"
     CDS             2545..2925
                     /gene="gcvH"
                     /locus_tag="PK742_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQU76512.1"
                     /translation="MQFPDNLKYTKDHEWVRVEGNTGYIGITDFAQRELGDIVYVDIT
                     GDGETLAMEDTFGTVEAVKTVSDLYMPVSGTISEVNPELDGAPELINQDPYGKGWMIK
                     VEMSDLSELDGLMDAAAYGELVAG"
     gene            2932..3303
                     /locus_tag="PK742_07695"
     CDS             2932..3303
                     /locus_tag="PK742_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQU76513.1"
                     /translation="MPKSRTFWFTLLWALFILFISVIPGEDLPSLSIWEADKLAHALV
                     YAMLCAGVILTLRIQNTWAKNRLKAIIFALVLCNLYGAGIECIQGLFLPSRTFDIYDL
                     MANGIGTLMGVTGSLFIFKPH"
     gene            3314..4132
                     /locus_tag="PK742_07700"
     CDS             3314..4132
                     /locus_tag="PK742_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQU76514.1"
                     /translation="MGSATFLVVLALLVVPVLAFVLIYRNRKPDDVIKEYAGTEEDRS
                     VFVKKYESADVNRQHTLFMEVGLSVSLAIILLAFAYAPKEDDTSSLMTVDAIEEEIEE
                     IPQTNQAKPPPPPPPPPPEVIEVEDDIEVEDQPNLFDQEMDVDVEIETQAYIPEEPVI
                     KKEEPKEPEIFVVVEQMPEFPGGDKALYEFLAKNIKYPAVAKDNGIEGKVYIKFVVNE
                     DGSVSQAQVVRGIGGGCDEEALRVVKDMPKWKAGKQRGKNVKVWYTLPFNFKLQ"
     gene            4192..4647
                     /locus_tag="PK742_07705"
     CDS             4192..4647
                     /locus_tag="PK742_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76515.1"
                     /translation="MAKRICTWSLIQIMLFGSIMAQTVCLMPPAFYDSDKPGIFEQMH
                     GFSGREKSPLEFLATTTVYPTFTQNNCPEWKMYVKFAIMADGSIDHVNVVRGFYPSCA
                     VRSVRVNSFLLSQITGMNEGYAVSVWYTIPYCFKLHEKSIPKESKEDQE"
     gene            4670..6490
                     /locus_tag="PK742_07710"
     CDS             4670..6490
                     /locus_tag="PK742_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76516.1"
                     /translation="MNRFLPYLLVLFLLPAARVFAQSNADRIKLAKEYELAGEFEKAA
                     PIYKDLYEDNPGSNSYYRMYYNILLTLQEYKDIDKLLNKQIKRDPENLTYYVDWGISL
                     RRQGNEAGAVEKFREALDAAGRSRTQYIQLANAFLTIDETDLAIETYEKGKKQVKDYS
                     FNSELANLYYRTGEYRRSMELYIDFAQEDPGRINSVTNGLQRMLDTEANHALLQEILY
                     ARIQQKDDPLLVEILVWDFIQQKDFASAFLQVKALDKRLRENGERVYDLGETARAEKD
                     YDAAISCYQYVIDKGEQSPYYYMSRNGVLNCRRTKIFETNVYTREDLLALKQDYLQFL
                     SDYNRSDYRSAFVTSELAQLEAFYLYDVDQAIERLEPVVQWQRLLPAQASELKLVLGD
                     LYLISGDVWEATLTYSQVDKAMKDEPLGEEARFRNAKLAYYRGDFALAQGQLNVLKSA
                     TSELVANDALKLSVFITENLGLDSVAEPMELFAAAELMFFQNRNNDALAVLKSLEEAY
                     PGHELLDDNYYLRFRIAMKEQNPEQAAEYLENIRQNLAYGLLADDAVFALGELYEEQL
                     NKPEEAKLCYEQIILQYTDSVYVTEARKRYRRLRGDANPG"
     gene            6515..6820
                     /locus_tag="PK742_07715"
     CDS             6515..6820
                     /locus_tag="PK742_07715"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HQU76517.1"
                     /translation="MYIYNVTLKVDATVADDWTRWMRQTHIPEVIDTGYFTGYRMCRL
                     LDDGDLDGITFVIQYECKDIDDFLAYQTTQAPGLQKAHAERYGETVIAFRTIMELLE"
     gene            6866..>7198
                     /locus_tag="PK742_07720"
     CDS             6866..>7198
                     /locus_tag="PK742_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQU76518.1"
                     /translation="MKKHVVVIGAGFSGLSAACELASNGFQVTVLEKNISAGGRAAVF
                     HDQGYTFDMGPSWYWMPDVFDWFFERYGSSVAEQYDLQRLDPGYRVFFGKDDIRDIPA
                     SLDDLYALF"
BASE COUNT         1993 a         1698 c         1727 g         1780 t
ORIGIN      
        1 ttcccatcag atttgggtat tcgcctgcca ctgtatgcca gtatttcaga atcattcagc
       61 aatccgcaat atgaccccta cacctttgat atcgatctga aggataaagt aaatgatata
      121 gaggtactgt atggagctga ttcagcaaaa gcttatcaga gacaggccat tgaatatgtc
      181 agcatcaaga gtatcaacct gaccaatatc cgaattgatc gccctaagtc ttccggtacg
      241 cccaagatct acagcatcca gaactttgat gccaccctgg cctattctca ggattacaac
      301 agcagtcctg tgctggaaca cgatatcata gaccggtata aagcatcgtt aggatacaac
      361 tatgccatac gtgccaagtc tgtcaagcct tttgagaaaa tggttgacaa gaaagatgca
      421 tggctggccc tggtaagaga cttcaacttc aacctgctgc ccacttccta cagcttccgc
      481 acggatatga acaggcaatt tggtgaaacc ctgatgcgtg atatttacgg cgatggtctg
      541 attcaaccca ccttcaataa gttcttcaac tgggatcgat tctatggcct taaactggag
      601 ccgttcactg ctatgaagat cgatttctca gccaccacta atacccgaat agatgagccc
      661 tatggtcgta tagatacacc ggaagagaaa gataccatct ggaataatat ccgtgatctt
      721 ggccgcacca tcaattaccg gcacagtgtg tctgccagct ataatgttcc cctgaataag
      781 attcctgccc tgagctggac atcaataaga actaattata atataaccta tggctggaca
      841 gccggctcac tgggtctggc agattcgctt ggacacatac ttaccaatac ccaggcaaaa
      901 accattaacg gagaactcaa cttccgtaac ctgtataata aatcaccatt cctcaagcaa
      961 tacaacaaca atactttttc gctgaagggt gacaagggcg gaagaggcgg caaagatgaa
     1021 ctcaatgcag ccgatgccga agatgataaa gacaagaaaa agaagattaa gaaggtcagc
     1081 ccgacagctg aggtcatggt caggttgttg ctgtctttga aacgggtaac catcaactac
     1141 acagagaact acagtactac cctgcctggt ttcatgctga caccagattt gatggggctt
     1201 gatctgaaag acggaccggc accgggatgg gattttgtca tgggtattca gccctctggc
     1261 aactggttgg attctgcagc ttccaaaggc tggatttcca gtgcaggtac attcaacagt
     1321 accttcctgc agaactattc cagaaacctg gatgtgagaa ccaatctgga gcctgttcct
     1381 gaccttcggc tcgatattaa cctgaataag agctacagtg agaaccattc tgaatttttc
     1441 aggaatatcg gcacccttga tgaggtagat tttgcccacc tcaacccgat ggacatgggt
     1501 agcctcacta tgagctacag catactgggt accatctttg ataaattgaa taccacagat
     1561 gtgaccgaga ctttcagaca gtttgaggcc aacagggtca ttatatctga gcgttttgga
     1621 aatgagaaca tctattctga tggtcagttc tttgacccgc aagacagtac cttcctcgag
     1681 aactactttg acggttatgg cccctattct caggatgtgc tcgtgccttc gttcatagca
     1741 gcctatcgtg atatagatgc ggccaccatt cccctcaatg tatttgaaca gatgccaagg
     1801 cccaactgga ggcttactta taccggtctt accaagatcg atgccatgaa gaagatcttc
     1861 agcaacttta ccattaatag cgcttatacc agtacactgg cagtgaacag ctatatgacc
     1921 aacctgaact attttggcaa ctattatctg tatgccaata cagtagatac cctgtcaggc
     1981 aattactatt ctcttttctc cattcccaat gtggtgctga gtgaacagtt cagtccgctg
     2041 tttggtgttg atgccacttt tgtaagcagc ctgagcacca aattcgattt tcgcaagacc
     2101 agaaccctca acatgagttt tgtggattac cagctcactg agatcaggtc cacagaagta
     2161 accgtaggac ttggttacac cacttccggt atcaccctgc ccatcaagat caaaggcaag
     2221 cggcctaccc tggagaatga tgtgaccttc cggttcgata tgtcttaccg tgatgacatt
     2281 accaccaact tccgcctcga ccagaatctg aacgaaccta caggagggct gaccgtcata
     2341 cagatatcgc ccacgataga ttatctggtg aacgaccggt tcaatgtgca gctgtatttt
     2401 gacagacagc aaaccatacc caagatatct accgcatatc ccatcaccac caccaatgca
     2461 ggtgtcaagg tgcgcatgag tctggcagaa taaggtcttt caggctgtaa aatttcgttt
     2521 atttttggct gcaaaatcta ttacatgcaa tttcctgaca acctgaaata caccaaagac
     2581 cacgaatggg tgcgtgtaga aggaaacacc ggttatatcg gcatcacaga ctttgctcag
     2641 cgtgagctgg gtgatattgt ctatgtggac attaccggcg acggtgaaac ccttgctatg
     2701 gaagacacgt tcggaactgt agaagctgtc aaaacggtgt ctgacctgta catgccggtg
     2761 agtggcacca tttcagaggt aaaccctgaa ctggacggtg cacccgaact catcaaccag
     2821 gatccatatg gaaaaggttg gatgatcaag gtggaaatga gtgacctctc tgagctggat
     2881 ggcctcatgg atgcagctgc ttacggtgaa ttggttgccg gataacagat catgccgaag
     2941 agccggactt tctggttcac cctgttatgg gcactgttca tcctgttcat ttcggtcatt
     3001 ccgggtgaag acctgccttc tttgtctatc tgggaagcag ataaactggc acatgccctg
     3061 gtgtacgcaa tgctctgtgc cggcgttatt cttacattaa gaatacagaa cacatgggca
     3121 aaaaacaggc tgaaagcgat aattttcgct ctggttttat gcaatttgta cggagcaggc
     3181 atcgaatgta tacaagggct cttcttacca agcagaacct ttgacatata cgacctgatg
     3241 gcgaacggaa tcggaaccct gatgggagta actggaagtt tatttatttt taaaccgcat
     3301 taaaagccaa cgtatgggat cagcaacatt tttagtggtt cttgccctgc tggtagtacc
     3361 tgtgctggca ttcgtcctga tctacagaaa ccgcaagccg gatgatgtga tcaaggagta
     3421 tgccggaacg gaggaagaca gatctgtctt tgtcaaaaag tacgaatcag cagatgtgaa
     3481 ccgtcagcac accctgttca tggaagttgg cctgagcgtg tcactggcca tcattttgct
     3541 ggcctttgcc tacgcaccaa aagaggatga cacctcctct ctgatgacag ttgatgccat
     3601 cgaagaagag atcgaggaaa tccctcagac caatcaggcc aagccacctc cacctcctcc
     3661 gcctccaccg cctgaagtga tcgaagtgga agacgatatc gaggtggaag accagcccaa
     3721 cctgttcgat caggagatgg atgtggacgt ggaaattgaa acccaggcct acattccgga
     3781 agaaccggtc attaaaaaag aagagccgaa agagcccgag atcttcgtag tggtggagca
     3841 aatgccggag tttcccggtg gcgataaagc cctctacgag ttcctggcca aaaacatcaa
     3901 atacccggcc gtagcaaaag acaacggcat tgaaggaaaa gtgtacatca agttcgttgt
     3961 gaacgaagat ggcagcgttt cgcaggctca ggttgtccgc ggtatcggtg gtggttgcga
     4021 tgaagaagcc ctccgcgtgg tcaaagacat gcccaaatgg aaggccggca agcagcgtgg
     4081 aaagaacgtc aaggtatggt acaccctgcc ttttaacttc aaactgcagt aagctgaaga
     4141 acaacatttt tcaaggcccc gcaaggggcc tttttttatc tttagagaca tatggcgaag
     4201 agaatttgta cctggagcct gatccagatc atgttgttcg gcagtattat ggcacagaca
     4261 gtttgtctta tgcctccagc tttttatgat tctgataaac cgggaatatt tgagcaaatg
     4321 cacggatttt ccggaagaga gaaaagtccg cttgagtttc tcgcaaccac cactgtgtat
     4381 ccaacgttta cgcagaacaa ttgcccggaa tggaagatgt atgtaaaatt tgccatcatg
     4441 gcagacggaa gtatcgatca tgtaaacgtg gttcggggat tctacccttc ctgtgctgtg
     4501 agatcagtca gagtaaattc tttcctgctg tcccagataa caggaatgaa tgaagggtat
     4561 gcggtcagtg tatggtacac cattccttat tgtttcaaac tccatgagaa aagtattcca
     4621 aaagaatcta aagaagatca ggaatgaatt ttgctgacgt tattatcaga tgaaccgttt
     4681 cctgccatat ctcctggtcc ttttcctgct gcctgcggcc agggtttttg cccagtctaa
     4741 tgcagaccgc atcaagctgg ctaaagagta tgagctggcc ggcgagttcg aaaaggcggc
     4801 tcctatctat aaggatctgt atgaagacaa ccccggttcc aattcctatt atcggatgta
     4861 ttacaatatc ctgcttaccc tgcaggagta taaggatatt gacaagctgc tgaacaaaca
     4921 gatcaaacgt gatccggaaa acctgaccta ttatgtagat tggggtatct ctttgcggcg
     4981 acaaggaaac gaagccggtg cggtagagaa attcagagaa gccctggatg cagccggccg
     5041 aagccgcaca cagtacattc agctggccaa tgcctttctg accatcgatg agacagacct
     5101 ggccattgag acctatgaaa aaggaaagaa acaggtcaaa gactattcct tcaacagtga
     5161 actggccaac ctgtattata gaacaggcga gtaccgcaga tccatggagc tttacataga
     5221 ttttgcccag gaagatccag gacgtatcaa cagtgtgacc aatggcctgc agcgcatgct
     5281 ggatacagaa gccaatcatg ccctgctgca agagatcctg tatgcccgca tacagcaaaa
     5341 agatgatccg ctgttggtgg agatactggt ctgggatttt atccagcaaa aagattttgc
     5401 ctccgcattc ctgcaggtca aagcattgga caaacgtttg cgggagaacg gagaaagggt
     5461 ctatgacctg ggtgagaccg ccagggcaga aaaagactat gatgccgcca tcagttgcta
     5521 tcagtacgtg atcgataaag gcgaacaatc gccttattat tacatgtcac ggaatggcgt
     5581 gctcaattgc cggcgcacca agatatttga aacaaatgtc tatacccgcg aagatctgct
     5641 ggcactgaaa caagattatc tgcagttcct gtctgactat aatcgttccg attaccggtc
     5701 ggctttcgtt acctcagaac tggcacaact ggaagcattc tatctgtatg atgttgatca
     5761 ggccatcgaa cggctggaac cggttgtaca atggcagcga ctcctgcctg cacaggcatc
     5821 tgaactcaaa ctggtgctgg gagatctgta tctgatatca ggcgatgtgt gggaagccac
     5881 cctgacctac tctcaggtgg ataaggccat gaaagatgaa ccgctgggtg aagaagcccg
     5941 tttccgcaat gcaaaattag cttactaccg cggcgatttc gccctggcac agggtcagct
     6001 gaatgtgctc aaatctgcta ccagtgaact ggttgccaac gatgcactga aactttctgt
     6061 attcatcaca gaaaacttag gcctggattc agtggctgaa ccgatggaac tgtttgccgc
     6121 tgctgaactg atgtttttcc agaaccgcaa caacgatgcc ttggcggtat tgaagagtct
     6181 ggaagaagct tacccgggtc atgaactgct ggatgataat tattacctcc ggttccgcat
     6241 agccatgaaa gaacagaacc cggaacaggc tgcagagtac ctggaaaata tcaggcagaa
     6301 cctggcatat ggtctgctgg ctgatgacgc agtttttgct ctgggcgaat tatatgaaga
     6361 gcaactgaat aaacctgaag aggccaaact ctgctatgaa cagatcatcc tgcaatacac
     6421 cgattcggta tatgtaacag aagctcgtaa gcgctaccgc aggctgcgtg gcgatgccaa
     6481 tccgggctga tgccttacct ttggcatact gactatgtat atctacaacg ttaccctgaa
     6541 ggttgatgca acggttgccg acgactggac ccgctggatg cgacagaccc atattccgga
     6601 agtgattgat acaggttatt ttaccggtta taggatgtgc cggttgctcg atgacggtga
     6661 tctggacggg atcacttttg tcattcagta cgaatgtaaa gacatagatg attttcttgc
     6721 ataccagacc acccaagcac ccgggctgca aaaggcacat gcggaacgct acggcgaaac
     6781 tgtgatcgcc tttcgcacca tcatggaact gctggagtga acaaactggc accagacctg
     6841 ttaagtattt ctttttcaaa aggatatgaa gaaacacgtg gtcgttatcg gagccggatt
     6901 cagcggcctc agcgcagcct gtgaactggc atctaatggc tttcaggtga ccgtgctgga
     6961 gaagaatatt tcagccggcg gcagggcagc ggtgtttcat gatcagggct atacatttga
     7021 tatgggcccg agctggtact ggatgcccga tgtattcgac tggttcttcg aacgttatgg
     7081 cagttccgta gcagaacaat atgacctgca gagacttgat ccgggttatc gggttttttt
     7141 tggaaaggat gatatcagag atatccccgc ctctctcgat gacctgtatg ccttgttc
//
LOCUS       DAOMAX010000126         7175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1592_length_7175_cov_3.512079, whole genome shotgun sequence.
ACCESSION   DAOMAX010000126 DAOMAX010000000
VERSION     DAOMAX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_7175_cov_3.512079"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..4748
                     /locus_tag="PK742_07725"
     CDS             33..4748
                     /locus_tag="PK742_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76519.1"
                     /translation="MSDYDSEAIAGGGPSAIREINYIESINTRYTGTEGLMGSRSSVK
                     GNVLLEGLELPAVGTNTSVGSIVHGNYVFNFVYNSRSGGYIYAYDKRDQTWYTVDTEI
                     PGWSPDLPIGAEVRNEKLLYWVNGREEGGKVYGSYPCKINVEKAVNNKPRVYRLYFER
                     SQVEDQTSTILTIGIYSPDFIEEFDAGILAGPYDNLQDWCEAMADQLNTLFTVATFEG
                     CGEYIEVTFSEVNDFYIGLALYQYGGLGAVGTMLAVPYNFFIEPREEYRSRVKYAPLC
                     QPTFEFGYDPEFTQNNLAKQYLQFAVQYIYADGEHTILSPASELAFINTPCGVDPDTA
                     FQNYIDINFYDPRLNDALTFSDLRRVAICVRLGNEGIWRLVKELERQDFDILTNKLRF
                     YNDGFYPAVSTDYYREDEYLPLISCGQAFSQNVGYLGGNLEGYDNLDCVDASIDVNYE
                     RTVECGKTGTVTGTIRIKNIWDGSTFGNNQPIHRPNAGGYVKFGGITPSGTPFSDSMA
                     DHQQTIKLGGFIVYAVGTDIWAKSVQNVPSGVTTIGGDDGTFDSSSISLRNAIRTAMN
                     NNEVYSTFELELPEGRHILRIASNLCSGDGVGAHDFQGGDWRSSSLPMHMSAATEIVV
                     DVVADSSVDAGTFVVADLVDTALLTESHAVIGYLIDSDVSSDRDDLKAGPRMERSSVE
                     DSNATYYGLDTPFTNGGCDHNGFFWYAKSATIGFNTSFQPKLGGGGIHNVGENFYTNF
                     LSALYDATALLSATTVSCGSSGDFRNVAPVCVWAKGDTAPWKVKVEGRLVDPSYGGVP
                     GVPVVIYNCTRVGVTDGAGYYSILTYRDWSYGTLKRDGWIVFVNDSGCCKTYTNGQYF
                     FFEWTSAYNFNLPYTAADWEVVINDFDNNLVWKRRNSIQWGIQYQDIAGRKGRVQTGK
                     DLHVFIPFWTESHGDDNPVLRFNINHEPPAWAYKWQLVRTKNQLISRFLQFIIYEAKY
                     IRYVDTVTGDPEPVETTYDAGDATEVHLNMRSIADYLARNQGFALGFVPDVGDRVSFI
                     KDADGILYEQLFDYRMSGYNGDLETASSPLTYIIPFDTSLPELVGGELIEVYTPKKQV
                     DGDNELFYECGECWDILDYGTGSPRHGGGTLGNDQDLDAGTPATGTITTGDVYLRSRN
                     MVIIDYDDEEVTEYDRNVEDEYLYEDVVASRQFSIGRSNYVDKEFKQLFLGGRVRFDQ
                     RFYSDGNKVFNGHNNFNAGDYVDLDASFGSITELVRIGETTELLAVMKKRIQPIFTSK
                     SPVYDFRGDQSVGKSESPLNVGTPLRRAVGSKHRLSIVQWDSIVWGFDEARAEFWQFA
                     QNDVFLLSTERGLKLETLRICEGLSQDEFSMVVGCFNPINEAVVWGIKGVTVSGSSEA
                     TAEAEAGVVVDTEVLAPITIEYSVKRGGFEGSFLFYPEILSNDGLRWYSFQGGLGHQE
                     SIGPVLTWYGVKSYASITAVFADFPNTEKDWFSVREKSNAVWHCPYIYIPSDAGIPNG
                     MTSRLTKGRFVKLEGDWFADFLRDMTDKTREGTEVEKLFNGRPLKGAVMVIEFRNADD
                     EIAALREINILVSPSAETV"
     gene            4759..5652
                     /locus_tag="PK742_07730"
     CDS             4759..5652
                     /locus_tag="PK742_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76520.1"
                     /translation="MPAPTLLAAIPEIARFGYGMFQDVKANQAAKQIERPEFKIPEAN
                     KEALQLSRVMAGIRSIPGLDGGDGERLLADTVRGLKSIGTVETGDVAKAAADLKKNQG
                     DQLSQFINGNTFHLIDALTGFGSLQAEAFAWNQQAKYQEEINAVSSISDAANKNMFTA
                     LSNASTIGLEKMTQDSWMNFFDDYYDKPMAKPPLVGDAKESSTGVGITLPENRGPGLG
                     FQRTAQSQQELPSITDSRVTEDFVPAFGDFKFGLPDQMGGTKYGVTKEKQGPTNLYDI
                     FDPTKMMMNTGLETVNPISIY"
     gene            5652..7151
                     /locus_tag="PK742_07735"
     CDS             5652..7151
                     /locus_tag="PK742_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76521.1"
                     /translation="MARLGRLIQSRAATVLPEGDHIGAFKRHGEAIIAQAKLMREKQA
                     AEQANLDKSLIPPDIDTEGMYAQDRKEIADDMDAYQTAAYEYYQQGINHMDPRNAEAY
                     QKMRGLEKNMELKAYTAKNHYKIDEEARKSMGKKDFHELYDVLGGVERLSVARDYGPD
                     NGGIMGREKYYQGIGYNLFDFKEYDFDQYFSDNKWDLTEWDDEPKIKGGSWWWNHNEG
                     YTEDQVDGMSKNFLTDPQTARWVNQRLSGLSERGKLSIMAQAAKNNTTLEEQFLRNEI
                     NSRQPVSQTLKSKSVGGKKGGSGGGDKEKDGGEMWLIQQFAAFYNGDDGIYDGTFTNK
                     GGDEYKYSLQPFAFSIGTFSFQENQDSPKTTIKQGALEATVEEGGSGKKWVKHDNYIE
                     AIAHGDDGVWVATTESNTKMSGIKQAVNGGGDMYWNKLTPKQADKLLSKMVQGNSKFS
                     MTKFDAITKGKRDAAGVLDLNYFYWQGVGGGEVSNQEGENNIPSAVAGK"
BASE COUNT         1904 a         1595 c         2010 g         1666 t
ORIGIN      
        1 aatggctgaa aaagatatta tctggcattt gggtgtcaga ctatgactcc gaggcaatcg
       61 ccggcggagg cccttctgca atacgggaga tcaactacat cgagagcatc aacaccaggt
      121 acaccggtac tgaggggctg atgggaagcc ggtcatcggt caagggtaat gttctactcg
      181 aaggactgga acttcctgca gtaggaacga ataccagtgt tggctctatt gtccatggca
      241 actacgtttt caatttcgtc tataacagtc gctcaggcgg gtacatctat gcttacgaca
      301 aaagagacca gacttggtac acggtagata cggagatacc aggatggagt ccggatctcc
      361 caatcggagc cgaggtgcgc aatgagaaat tgctgtactg ggtaaacgga agggaggaag
      421 ggggaaaggt ttacggtagc tatccctgca agatcaatgt tgagaaggcc gtaaacaaca
      481 agccccgtgt gtaccggctg tactttgagc gcagccaggt agaagatcag acttctacca
      541 ttctgaccat tggcatatac agccctgatt ttattgagga attcgacgct ggtattctcg
      601 cagggccgta tgacaatctg caggactggt gcgaggcgat ggccgatcag ctgaacacat
      661 tgttcacggt cgccactttt gagggttgtg gagagtatat cgaggtcact ttctccgaag
      721 taaatgattt ctacataggg cttgcgctgt accaatacgg aggtctgggt gctgtcggca
      781 cgatgctggc ggttccctac aactttttta tcgagccgcg ggaagaatac cgtagcagag
      841 tgaaatatgc tccgctgtgc cagccgacat tcgaattcgg ctatgatcct gagttcaccc
      901 agaacaatct ggcaaagcag tacctccagt tcgccgtgca gtacatctat gccgatggag
      961 agcacaccat actgtcaccg gcttccgagc tggcattcat caataccccg tgcggagtgg
     1021 atcccgacac ggctttccag aactacattg acatcaattt ctatgatcca aggctgaatg
     1081 atgccttgac tttttccgat ctgcggaggg tggccatctg tgtcaggctg ggtaatgaag
     1141 gtatttggcg actggtgaag gagctggaga ggcaggattt tgatatcctc accaataagc
     1201 tgagatttta caatgacggg ttctatcccg ctgtttccac cgattactac cgggaagacg
     1261 agtacctgcc cctgatcagc tgcgggcagg cattcagcca aaacgtgggc tatctgggcg
     1321 gcaacctgga agggtatgac aacctggact gcgtggacgc cagcattgac gtgaactacg
     1381 aaaggacggt ggagtgcggt aaaacgggta cggtcaccgg aaccatcagg atcaagaata
     1441 tctgggatgg atcaaccttt ggcaacaacc agcctattca tcggccaaac gccggaggat
     1501 atgtgaaatt cggggggatc acgccgagtg gaactccttt ttccgatagc atggccgacc
     1561 atcaacaaac catcaagctc ggtggattca ttgtatatgc tgttggcacg gatatctggg
     1621 ctaaatcagt tcaaaacgtt ccatccggag taacaaccat cgggggtgac gacggcacat
     1681 ttgacagcag ctctatctcg ctgagaaatg cgatacgtac cgccatgaat aataatgagg
     1741 tgtacagcac ttttgagctt gagctcccgg aaggtcgtca tatccttcga attgccagca
     1801 acctttgttc tggggatggg gttggtgcac atgatttcca aggcggcgac tggagatcct
     1861 cttctctgcc gatgcatatg tccgcagcta ctgagatcgt cgtagatgtc gttgcagata
     1921 gttcagtgga tgccggcacg tttgtcgttg cagatctggt agatacagct cttttgacgg
     1981 agtcgcatgc agtaatagga taccttattg attccgatgt cagcagcgat cgtgacgatc
     2041 tgaaggcagg gccgagaatg gagagatcca gtgtggagga ttctaacgca acttactacg
     2101 gccttgacac gccgttcacc aatggcgggt gtgatcacaa cggttttttc tggtacgcca
     2161 aatcagccac aataggcttc aatacaagtt ttcagccaaa actgggcggt gggggcattc
     2221 acaatgttgg agagaacttc tacacgaatt ttctctcagc gctatatgat gctactgcgc
     2281 tgctcagcgc cacaactgtc agttgtggat catctggaga tttccgcaat gtcgctccgg
     2341 tatgtgtatg ggcgaaagga gacactgctc catggaaggt aaaagtggag ggcagactgg
     2401 tagatccaag ttatggaggc gtgccaggcg tgcccgtagt gatttacaac tgcacaagag
     2461 tgggcgttac cgatggcgca ggatattact ctatcctcac ctatcgggat tggagttatg
     2521 gcacgctgaa gcgtgatggc tggatagtat ttgtcaatga cagtggttgc tgcaagacct
     2581 acaccaatgg ccagtacttc ttcttcgaat ggacatcagc atacaatttc aacctaccat
     2641 atacagcagc agactgggag gtggtgataa atgacttcga caataactta gtgtggaagc
     2701 gaagaaactc tatacagtgg gggattcagt accaagatat tgccggcagg aaaggccggg
     2761 tgcagactgg caaggatctg catgtattta taccgttctg gaccgaaagc catggtgacg
     2821 acaatcccgt gcttcggttc aacatcaacc atgagccgcc ggcatgggct tacaagtggc
     2881 aattggttag aaccaagaat cagctgatat ccagattcct gcagttcatc atctacgaag
     2941 ccaagtatat ccgatacgtg gacactgtta ccggagatcc tgaaccggtc gaaaccactt
     3001 atgatgcagg tgatgccaca gaggtgcact tgaacatgcg gagtatcgct gattatctgg
     3061 cgagaaatca gggctttgcc ctgggctttg tacctgatgt cggagatcgt gtttccttca
     3121 tcaaggatgc ggatggcatt ctctacgaac aactgttcga ctaccgtatg tccgggtata
     3181 acggtgattt ggagactgcg agctctccgc tgacctatat catccccttt gacaccagcc
     3241 tgcccgaact ggtgggcggg gaactcatcg aggtctatac cccgaagaag caggtggatg
     3301 gagataatga gttgttctat gagtgcgggg agtgttggga tattctcgac tatggcacag
     3361 gctctcccag acatggaggt ggcacgctgg gtaatgatca ggatcttgat gcgggtacac
     3421 ctgcaactgg cacgatcacg accggagacg tatatctgcg ctccagaaac atggtcatca
     3481 ttgactacga tgatgaagaa gtaaccgagt atgacagaaa tgtagaggac gagtatctgt
     3541 atgaagatgt cgtggccagc aggcaattca gtattggccg gtccaactat gtggacaaag
     3601 agttcaagca gctgtttctg ggaggtcggg ttcgatttga tcaaaggttt tacagcgatg
     3661 gcaacaaggt cttcaatggc cacaataatt tcaatgccgg cgactacgtg gacctggacg
     3721 ccagttttgg cagcatcacg gagctggtaa ggataggaga aaccacggag ctgttggctg
     3781 tgatgaagaa gcggatacag cccatattca cgtcaaaatc tcctgtttat gatttcaggg
     3841 gggatcaatc tgttggcaaa tcggagtcac ccctgaatgt gggaacgccg ttgcgccggg
     3901 ctgtcggtag taagcacagg ctttccatcg tgcaatggga cagcattgtc tggggctttg
     3961 atgaggccag ggcggagttc tggcagtttg ctcagaatga cgtgttcttg ctctccacag
     4021 agagaggtct gaagctggag acgctgagga tctgtgaggg cttgtcacag gacgaattca
     4081 gcatggtcgt tggctgcttc aatcccatca atgaagccgt agtatggggg ataaaaggcg
     4141 tcactgtttc tgggtcttct gaggcaactg ctgaagcgga agcgggcgtt gtggttgaca
     4201 ccgaggtgct ggctccgatc actattgaat acagtgtgaa gcgaggcggg tttgaaggct
     4261 ccttcctgtt ttatccggag atcctcagca atgatgggct gcgctggtat tcctttcagg
     4321 gggggcttgg tcatcaggag agcatcggtc cggtgcttac ctggtatggc gtgaagtcct
     4381 atgccagcat cacggctgta tttgccgact tccccaatac cgagaaagac tggttcagcg
     4441 tgcgggagaa gtccaatgcg gtctggcact gcccatatat ctacatccct tccgatgccg
     4501 gcataccgaa tggcatgacc tctcgtttga caaaaggaag gttcgtgaag ctggagggcg
     4561 actggttcgc agatttcctc cgggacatga cggacaagac cagagagggt acggaagtgg
     4621 agaaactgtt taatggcagg cctttgaagg gtgctgtcat ggtcatagaa ttcaggaatg
     4681 cagatgatga aatagcggct ttgagagaaa tcaacatttt ggtttctcct tcagctgaaa
     4741 ccgtgtaaat ttgttgttat gccagctcca acacttctcg cagcgatccc tgaaattgcc
     4801 agatttggat atggaatgtt tcaggatgta aaagctaatc aggccgcaaa gcaaatcgag
     4861 cgccctgagt tcaagatccc tgaagccaat aaagaggccc tgcagctgtc tcgcgtaatg
     4921 gcaggcattc gcagcatacc cgggctggat ggtggtgatg gagagcgctt gctcgcagat
     4981 accgtcaggg gtctgaaaag cataggcaca gtggaaaccg gcgatgtcgc caaagctgct
     5041 gccgatctga aaaagaacca gggagatcag ctttctcagt tcatcaacgg caacaccttt
     5101 cacttgatcg atgccctcac cggcttcggt agtcttcagg cagaagcctt tgcctggaac
     5161 cagcaagcca aatatcagga ggagatcaat gccgtctctt ccatttctga tgccgcgaat
     5221 aaaaacatgt tcactgcgct ttccaatgct tcaaccatag gtctggaaaa aatgacgcag
     5281 gactcatgga tgaatttttt tgacgattac tacgataaac ccatggcaaa gcctcctttg
     5341 gttggcgatg caaaagaatc ttctaccgga gtgggcataa cgctaccgga gaaccgcggc
     5401 ccgggtctgg gcttccagcg caccgcacag tcgcagcagg aacttccttc catcaccgat
     5461 agcagggtga cagaagactt tgttcctgcc tttggcgact tcaaattcgg cctccccgat
     5521 cagatgggag gaactaaata cggtgtgaca aaagaaaagc agggcccgac aaacctgtat
     5581 gacatttttg atcccacgaa gatgatgatg aataccggtc ttgaaactgt gaaccccata
     5641 agcatatact aatggcgagg ctgggtaggc ttatacaatc tcgtgccgct accgttctac
     5701 cggaaggaga tcatattggc gcttttaagc gtcacggtga ggcaattatc gctcaggcaa
     5761 agctgatgcg ggagaagcag gcggctgagc aggcaaacct ggacaagagc ttgatacccc
     5821 ctgatatcga cacagagggc atgtacgctc aggatcgcaa ggagatcgct gacgacatgg
     5881 atgcatacca gacggcggcc tacgaatatt atcagcaggg catcaaccac atggatcctc
     5941 gcaacgcaga agcctaccag aagatgcggg gactggaaaa gaacatggag ctgaaggcct
     6001 acacagccaa gaaccactat aagattgatg aagaagcccg gaagtctatg ggcaaaaagg
     6061 atttccacga actctatgac gtactggggg gagtggagag actgtctgtt gccagagatt
     6121 acggtcctga caacggtggg atcatgggca gggagaagta ttaccagggc ataggctaca
     6181 atctgttcga cttcaaggaa tacgattttg atcaatactt cagcgataat aagtgggatt
     6241 tgacggaatg ggatgatgaa cccaaaatca aaggtggtag ctggtggtgg aatcacaatg
     6301 aagggtacac cgaagatcag gttgatggga tgagtaaaaa ctttctgaca gatcctcaga
     6361 ctgctcgatg ggtgaatcaa cggttatctg gactaagcga gcgcggcaag ctgtcgatca
     6421 tggcgcaagc ggctaaaaat aacacaaccc tggaggagca gtttttgcgg aatgagatta
     6481 attctcgcca accggtaagc caaacgctta aaagcaaaag cgtaggaggt aaaaagggcg
     6541 gcagcggtgg tggtgacaag gaaaaagatg gcggcgaaat gtggcttatc cagcagtttg
     6601 cagccttcta taatggcgat gatggtattt atgatgggac ctttacaaac aaaggcggtg
     6661 atgaatataa atattcgctt cagcctttcg ctttctctat aggaacattt tcattccagg
     6721 aaaaccagga ttccccaaag acaactataa aacagggggc tcttgaagct accgtggaag
     6781 aggggggatc cggcaaaaaa tgggtaaagc atgataatta cattgaagct atcgctcatg
     6841 gggatgatgg tgtttgggtt gcgacaacag agagtaacac gaaaatgagc gggattaagc
     6901 aggctgttaa tggcggtggt gatatgtact ggaataagct gacaccaaag caggccgaca
     6961 aactcctgtc taagatggtt cagggcaatt ctaagtttag catgaccaag tttgatgcta
     7021 tcacaaaagg aaagagggat gctgccggag tgctggatct aaattacttt tattggcaag
     7081 gggtgggcgg cggcgaggtt agcaaccaag aaggggaaaa taatattccg tcagcagtag
     7141 ctggaaagta gtaaataaaa atatcatggg taaat
//
LOCUS       DAOMAX010000127         7161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1595_length_7161_cov_3.731635, whole genome shotgun sequence.
ACCESSION   DAOMAX010000127 DAOMAX010000000
VERSION     DAOMAX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1595_length_7161_cov_3.731635"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PK742_07740"
     CDS             <1..575
                     /locus_tag="PK742_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQU76522.1"
                     /translation="LQYSEERSSVFHVKAHWALYCDNYLEGFHIPYVHQALNKVIDYG
                     QYETHLFDSGTLQIGIGKEGEPCFDLPAGHPDQGKQVAAYYYWFFPNWMVNVYPWGIS
                     VNIVEPIDIGQTRVRFLTYVARPEMLDQGAGSGLHAVEMEDEEVVESVHRGLTSRLYR
                     DGRFSPAMETGVHHFHLLLQHHMADFLLNN"
     gene            697..3945
                     /locus_tag="PK742_07745"
     CDS             697..3945
                     /locus_tag="PK742_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76523.1"
                     /translation="MKVRFTYLMLVLTMVCSTAFGQGVTTGSMNGLVKGSDGETLPGA
                     NVVAVHTPTGTSYGTVTREDGRFNLPNIKVGGPYTVTISFIGYENYVQNGLNINLGQD
                     LYLAADMKESGVTLQAVEINAGSDVINADRTGAKTVVDEVAINTVPTIQRDIADFAKL
                     TPQASVNGEGINIANTNNRFNAIFIDGAVNNDVFGLAASGTNGGQTGISPIALDAIEQ
                     FQISVAPYDVTLGGFAGGGINAVTRSGTNQYEGSVYFYTRNQNFTGRTNPYVFDPQDT
                     LALDDEALDTLRTAVPDYTSNIMGIRVGGPIIKDKAFFFVNAELQREETPQPFVFDNY
                     LGDLEESDITGLQNYLSSVYGYDAGGFLNNTRLLTSDKVLAKIDWNLSDKHKLSIRHS
                     YVKGVSTSPAASTPTTINFENGGIYFPSTTNSSALELNSLFSNTMSNNLIIGYTNVND
                     DRDAMGADFPRVQIRDGSANIFFGSEEFSTGNQLTQSIFTITDNLKLYKGQHTFTIGT
                     HNEFYHIYNLFIRQAFGSYDYLSLQDFYDDATPNDYAVSYSLVDDVVGDGSAAAADFN
                     AMQLAVYGQDKYQASEKLSVTAGIRFDLPVYMTDPFVDTYFNDTAAANIEAEGYDLGG
                     AQAGQMPGASLLFSPRVGFNYDVMGDQTFQIRGGIGIFTSRIPWVWPAGAYTNSGVIV
                     GGLFKNTAAIESELPEFTFNPDPYTQYTLADFGGTDAVPQGQLDLFVEDFKFPQVLRG
                     SVGVDKKLPWDLYGTVEFIYTKTLNNVVYYNVNQAMPDGNLTGTDNRPYWPSTKIESQ
                     YSRIILGDNTNEGSGYTFTAQLQKNFNRNFTASVAWSYGDAYAINDGTSSQNSSQWRF
                     MENVNGRNDLALTRSDFSMGHRVVGFISKRFEYTDNLATTLALYYNGQSGRPFSYVYN
                     LSVQGDDSGVADLIFVPGSQNDIVLVQTDAAGTVEEQWDALDAYISNDEYLNSRRGNY
                     AERNGDRTPFENMFDLKVVQDIQIKTGDNLNTLQLSLDILNFGNLLNARWGNDYFLVN
                     DNYSLIRFEGWQDGESGGTPTFSFVEPKNDIWGLNDLTSRWRMQLGVRYIFN"
     gene            complement(4400..6622)
                     /locus_tag="PK742_07750"
     CDS             complement(4400..6622)
                     /locus_tag="PK742_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76524.1"
                     /translation="MVRQLLRTFLLVILSSPTLFSQEVMMAWTPNLTDFPSHRVTDQH
                     ALQQLSSLEDHPEYGILPYQSPCESCAEVIEKRTPDSRYFISTIDPNEIYSQQFAGTS
                     AFHDPNGFLRSLDPRLYPAGDGLYRSTWQYTPSFLDIPNKQTGMESPSGNLLFNRDLE
                     LIHRRADGTEEMLGLADWSQYTVGENGIKVTDIWPGIDLIIAFQLETVKSNFEIRINP
                     GLTGGQLIIRDHLDLPAGYSIEPDEGQWTDTQSWRGNLLISAHGTRTVNWTIDPAYTY
                     DASGTRDGQLVCNYELSSDNALSVIIPIHWMNDPSLIYPLTIDPLVTSSATYSSGSME
                     FRFNGEWCGMTTNNCIYSLTVTKPANCTITGATFAAEYRSVVGAGGCGACWMSDAGFY
                     MEGPCGRSPISPFFWSCNLASTGTCSASGYAMDETVDCLTPECNGTVTFDIFNSYCYC
                     AANGTDCTGTLPCQRMNNNTWSITLKGHNLETLGDAADGSGSLSVGPDCCITTVLDPT
                     PDYGVPPYTYVWTDGSTSSDTTVFSCGDGVWVYTCDVTDACGITRTATFTMTVDDCGV
                     LPIELVQFSAVPVGQKVLISWKTESEWNNLSYLVEHSADGFTFHSIGEIAGAGNSAES
                     RQYELFDENPVVGTNYYRLTTIDTKGESNVSGLTDVLIADNKNFQFFPNPFYSSFTLV
                     LPESATVCITSSSGQVVWKSVSEKGQYFIDGSAWPDGLYQISYYGQENLVQTIFKSGN
                     "
     gene            6859..>7161
                     /locus_tag="PK742_07755"
     CDS             6859..>7161
                     /locus_tag="PK742_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76525.1"
                     /translation="MILATNAIAQVPDDVFQFAMGGTLSEADGNILALDDGGLIMVCE
                     SNSPVGGIKSEAIGSYDVWVLRMDAERNIVWDHCYGGGNNEYNPRIVQAGDGGFIIA"
BASE COUNT         1836 a         1671 c         1771 g         1883 t
ORIGIN      
        1 atcttcagta cagcgaagaa cgttccagcg tatttcatgt aaaagcacac tgggcccttt
       61 attgcgataa ctacctggaa ggatttcata ttccctatgt tcatcaggcc ctgaacaagg
      121 tgatcgatta cggtcagtat gagacccacc tgtttgatag tggtacctta caaataggta
      181 tcgggaaaga aggagaacct tgttttgacc ttcctgccgg acatccggat cagggaaagc
      241 aggttgcggc ttactactat tggttcttcc cgaactggat ggtcaatgta tatccatggg
      301 gcatctctgt gaatattgtt gaacccattg atattggaca gaccagggtg cgttttctga
      361 cctatgtggc cagacccgaa atgctggatc agggtgcagg ttccggttta catgccgttg
      421 aaatggaaga tgaagaagta gtggagagcg tgcaccgcgg gctgacctcc aggctctaca
      481 gagatggcag gttttctccg gcaatggaaa caggggtgca tcattttcac ctgctcttac
      541 agcaccatat ggccgatttt ctgttgaata attaagagag gtgcatattg ggtggataat
      601 tcactttttc cacatgttca tttgtccttg tgttaacgat ttgttaacga ggtagctcta
      661 ccttagcaac gaatttccaa cctaaaacaa atgactatga aagtacgttt cacctatctg
      721 atgctggtgt tgaccatggt ctgttcgact gcgttcggac agggtgtgac caccggctct
      781 atgaacggtc tggtgaaggg ttctgacgga gaaacgctgc ccggcgccaa cgttgtggcg
      841 gtgcacaccc cgacaggcac ttcttatggt accgtaaccc gtgaagacgg acgctttaac
      901 cttcccaaca tcaaggtagg cggcccttac acggtgacca tttcttttat cggttatgag
      961 aactatgtac agaacgggtt gaacatcaac ctcggtcagg acctctatct cgctgccgac
     1021 atgaaagaat caggcgtgac ccttcaggct gtggagatca atgccggctc tgatgtgatc
     1081 aacgctgacc gcaccggtgc caaaaccgtg gtggatgagg ttgccattaa cacggtaccc
     1141 accattcagc gtgacattgc cgatttcgcc aagctgaccc cgcaggccag tgtgaacgga
     1201 gaaggcatca acatcgccaa caccaacaac cgtttcaatg ccatcttcat tgatggagcg
     1261 gtgaacaatg atgtgtttgg tctggcagct tccggtacca acggtggcca gacgggtatt
     1321 tcgcccattg ccctggatgc gatagagcag ttccagatat ctgttgcacc ttatgatgtg
     1381 accctgggag gttttgccgg tggtggtatc aacgctgtaa cacgttccgg caccaatcag
     1441 tatgaaggtt cagtgtattt ctataccagg aaccagaatt ttacaggccg caccaaccct
     1501 tacgtttttg acccgcagga tacgcttgca ctggatgatg aggcactcga tacgctgcgt
     1561 accgctgtac ctgattatac atccaacatt atgggtatcc gggtaggagg ccctatcatc
     1621 aaagacaagg ctttcttctt cgtaaatgct gagttgcagc gtgaagagac cccgcagcct
     1681 tttgtattcg ataattatct gggtgatctg gaagaaagcg acattacagg tttacagaat
     1741 tatctgtcat ctgtttacgg ctatgatgca ggtggttttc tgaataacac cagattgctc
     1801 acttcagaca aggtactggc caagatagac tggaacctga gtgataagca taaactgagt
     1861 atccgccaca gctatgtgaa aggagtttct acctcaccgg ctgcttctac tcccactacc
     1921 atcaactttg agaatggcgg tatctatttc cccagcacca ccaacagttc tgctctggaa
     1981 ctgaactcac tcttcagcaa taccatgtcg aacaacctga tcattggtta taccaatgtg
     2041 aacgacgaca gggatgccat gggtgcagac tttcccagag ttcagatccg tgatggttct
     2101 gccaatatct tttttggttc tgaagaattc tccaccggta accagcttac ccagagcatt
     2161 tttaccatta ctgataacct gaagttgtat aaaggtcagc acacctttac aataggtaca
     2221 cataacgaat tctatcacat ctacaacctg ttcattcgtc aggcatttgg ttcttatgac
     2281 tacctgagtc tgcaggactt ctatgatgat gccacaccga acgactatgc agtgagctat
     2341 tcactcgtgg acgatgtagt gggtgatggt tctgctgctg ctgcagattt caatgccatg
     2401 cagctggcag tatatggtca ggacaagtac caggccagcg agaaactgtc tgtaactgca
     2461 ggtattcgtt ttgatctgcc ggtttacatg accgatcctt ttgttgacac ctacttcaat
     2521 gatacagctg ctgccaatat cgaagcagaa ggttatgacc tgggtggtgc gcaggcagga
     2581 cagatgccag gtgccagcct gctgttcagc ccaagggttg gattcaacta tgatgtaatg
     2641 ggcgaccaga ccttccagat ccgtggtggt attggcatat ttacaagccg tattccctgg
     2701 gtttggcctg ccggagccta taccaacagt ggtgtgattg taggtggtct gttcaagaac
     2761 accgcagcaa ttgaatctga gcttcctgaa ttcactttca atcctgatcc atatacacag
     2821 tacacccttg ctgactttgg cggcactgat gctgtaccgc agggtcagct ggatcttttt
     2881 gtggaagact tcaaattccc gcaggttctt cgtggctctg ttggagttga caagaagctg
     2941 ccatgggatc tgtatggtac agtagaattc atctatacca agaccctgaa caatgtggtc
     3001 tattacaatg taaaccaggc tatgccggat ggtaatctga ccggaactga taaccgccca
     3061 tactggccca gcactaagat agaaagccag tacagcagga tcattctggg tgataacacc
     3121 aatgaaggct caggttacac tttcactgcc cagctgcaga agaactttaa ccgcaacttt
     3181 actgccagtg tggcatggtc ttatggcgat gcctatgcca tcaatgatgg tacatcttct
     3241 cagaactctt cacagtggag gttcatggag aatgtaaatg gtcggaatga tctcgctctg
     3301 actcgttctg atttctctat gggtcaccgt gtggtgggtt tcatcagcaa gaggtttgaa
     3361 tacacagata atctggctac cacacttgcc ttgtattaca atggacaaag tggtcgtccg
     3421 ttctcatacg tatataacct gtctgtacag ggagatgaca gtggcgtagc tgatctgatc
     3481 tttgttccgg gaagccagaa cgatatcgtg ctggtacaaa ccgatgcagc cggaactgtg
     3541 gaagaacagt gggatgcact ggatgcctac atcagtaatg atgaatacct gaacagccgt
     3601 cgtggtaact atgctgagcg aaatggagac cgcactccgt tcgaaaacat gtttgacctg
     3661 aaagttgtgc aggatattca gataaaaaca ggagacaacc tgaacaccct tcagttgtca
     3721 ctggatattc tgaacttcgg aaacctgctg aacgcccgct ggggtaatga ctacttcctg
     3781 gtgaacgaca actacagtct gattcgcttt gaaggatggc aggatggcga aagtggcggt
     3841 actcctacct tcagtttcgt agaaccgaag aatgacatct ggggactgaa tgacctgacc
     3901 agtcgctgga gaatgcagct gggagttcgt tacatcttta actaagacca gaaaatccga
     3961 tttttaaaga gtcgcccacg ggcgactctt ttttttgggg gggtgagcag tgagtagttg
     4021 aatattggac aggcaagtta gaagatccgt tgtgtctcct ggcacactgt tactttttcc
     4081 attccgcatt ccctgcctaa cggcaggcag gcgcctttca ggtacctgat actcattcta
     4141 acaaacgaag aaagcggacc cgaacgtgca gtccgaaccc gctttcgcag atgctttgtc
     4201 cgtgaacaaa ctacctgcat ctgaacttcg tcagcattcc tttctttcgt ccggaactgc
     4261 cttcctttcg gttggtgggc taccttgcgg ttctgccctc tgatcattca ggtattccaa
     4321 acatactgca agagtgcacg caagtcaata gtcagtgacc cgattttctg ttcatcaaat
     4381 gcacaaaagc ggaacaaggt caattgcccg acttgaagat cgtctgaacc aggttttcct
     4441 gtccgtaata agatatttgg tacaacccat ccggccatgc tgatccgtca atgaaatact
     4501 gccctttttc agaaacgctc ttccatacaa cttgtccgct gctgcttgta atgcataccg
     4561 ttgctgattc aggtaagacc aatgtgaacg aagagtaaaa cgggtttgga aagaactgga
     4621 agtttttgtt gtctgcaata agcacatcgg tcagaccgct tacatttgat tcgcctttag
     4681 tatctatagt ggtcagccga tagtaattgg tacccaccac cggattctca tcaaagagct
     4741 catattgcct ggattctgca gagttaccgg caccggcaat ctcaccaatg gagtggaagg
     4801 taaaaccatc ggccgaatgt tcaaccagat aagaaaggtt attccattcg ctttccgtct
     4861 tccagctgat caacaccttt tgacccaccg gcacggcact gaattgaaca agttctattg
     4921 ggaggacacc acaatcatca accgtcatag tgaatgtggc agttcttgta ataccgcatg
     4981 cgtcagtaac atcgcaggta taaacccaaa cgccgtctcc gcaggaaaat actgttgtat
     5041 ctgaactggt tgaaccatca gtccaaacgt atgtataagg aggcactcca tagtcgggtg
     5101 tgggatccag tactgtggtg atgcagcaat caggtcctac tgacagagag ccgctgccat
     5161 ctgctgcatc tcccaatgtt tccaggttat gacctttcag agtgatgctc caggtgttgt
     5221 tgttcatgcg ctggcagggt agcgttccgg tgcaatctgt tccattggca gcgcagtagc
     5281 aatagctgtt aaagatgtca aatgtaacgg tgccattgca ttcaggggtc agacagtcaa
     5341 cggtttcatc catagcgtag cctgaggctg aacaggtgcc tgttgatgcc agattgcaac
     5401 tccagaaaaa gggcgaaatg ggagatctgc cgcagggccc ttccatgtag aatcctgcgt
     5461 cagacatcca gcatgcaccg catccgcctg cacccactac agaacggtat tctgctgcga
     5521 aagtggctcc ggtaattgta cagttggccg gcttggtaac cgtcagactg taaatacaat
     5581 tgttggtggt cataccacac cattctccat tgaaacgaaa ttccatggat ccggacgagt
     5641 aggtggccga gctggttacc agtggatcta ttgttagcgg atagatcaga gaagggtcat
     5701 tcatccaatg aatgggaatg atcacagata aggcattatc agaagatagt tcataattgc
     5761 aaacgagctg gccatcgcgg gtaccggagg catcataggt ataggcagga tcgatggtcc
     5821 agttgaccgt ccgggtacca tgggcagaga tcagcaggtt tccccgccag gattgtgtgt
     5881 ctgtccattg gccttcatca ggttcaatgc tataaccagc aggcagatct aaatggtcgc
     5941 gaatgatcag ttgcccgcct gtaagacccg ggttgatcct gatctcaaag ttgcttttga
     6001 cagtctccag ctgaaaagca ataataaggt caatgcctgg ccagatatcg gtcactttaa
     6061 ttccattctc accaactgtg tactgcgacc agtcagccag cccaagcatt tcttctgtgc
     6121 catcagcacg cctgtgtatc agttccagat cgcgattaaa aagcaagttt cctgatggag
     6181 attccattcc cgtttgttta ttgggaatat ccagaaatga cggggtgtat tgccaggtgc
     6241 ttcggtacag gccatcgccg gctggatata atcgaggatc gagggaacga agaaaaccat
     6301 tcgggtcatg aaatgcagag gtgcctgcaa attgttgtga gtagatctca ttcggatcta
     6361 tggtgctgat aaagtatctg gaatcaggag ttcttttttc aatgacctct gcacatgatt
     6421 cacatggcga ctgataaggc aatatgccat attcaggatg gtcctcaaga gaggataatt
     6481 gctgcaacgc atgctgatca gtgacccggt gtgacgggaa atcagtcagg ttgggcgtcc
     6541 acgccatcat cacctcctgc gaaaacaaag tcggggagga gagtataacc aacaagaaag
     6601 tgcggagtaa ttgtctgacc ataggattgg ggtttgagga tggccactct gcagcagggt
     6661 ctgtatttct ttccgaaagt taaacaatca ttaaccgaat atcaaaatca gccgatccag
     6721 gttttctgca agacaattgt cagatggttt ccagcagtta tcctgtatca tgtgttacaa
     6781 gtcagttcta atggcggatt gcttaatttt aacagattag acctagcaca catgcgtaac
     6841 tccttatgcg ggctgttgat gattttagca acaaatgcga tagcccaggt acccgatgat
     6901 gtatttcagt ttgccatggg gggcactctt tcagaggcag atggcaatat tctggccctt
     6961 gatgatggcg gattgatcat ggtttgtgag tccaactctc cggttggagg gatcaaatct
     7021 gaagccattg gctcctatga tgtatgggtc ttgcgcatgg atgctgaaag aaatattgtg
     7081 tgggatcatt gctacggcgg tgggaacaac gaatataacc cgcgtatcgt tcaagcaggc
     7141 gacggtgggt ttatcatagc g
//
LOCUS       DAOMAX010000128         7146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1598_length_7146_cov_3.903399, whole genome shotgun sequence.
ACCESSION   DAOMAX010000128 DAOMAX010000000
VERSION     DAOMAX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_7146_cov_3.903399"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PK742_07760"
     CDS             complement(<1..289)
                     /locus_tag="PK742_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU76526.1"
                     /translation="MDTTKKKRYRVFLVDDDTKHLVMLKNHLEKKSDYDLEINIFSSG
                     EACLERIQENPDVVVLDYYLDGIRTDAANGLEILRKILRANPGQDVIMMSGQ"
     gene            complement(298..2328)
                     /locus_tag="PK742_07765"
     CDS             complement(298..2328)
                     /locus_tag="PK742_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU76527.1"
                     /translation="MTNLHAAMEENLLIQKLRELLARDFVHRKSIESDLYRSEEKYRM
                     LIDQLTDAIYLSTLDGTFTGCNRALLEMLGYSYKELMQLSVSAIYAHPDDRRHLLQQL
                     IDKGEVRDYEVQLKRKNGELIDCLVNSSVRRDQNHEIVGFQGIIRDITLRKKTFELQR
                     AKDLAERANKFKAEFLANMSHEIRTPMNAIGGMLQLLNKTNLEPKQHEYLDGMQTAVD
                     SLLQIINDILDFSKIEAGKMELDNRPFSLQQTVEAIISTIRFKAEEKGLELVLDMDPS
                     IPPNLQGDPLRINQILLNLISNAIKFTPEGEVRVTIRLIDKLDHKARLFFSVKDTGIG
                     IEQEKLQDIFESFTQASTDTTRLYGGTGLGLAIVKKLIDMLNGAIMVRSRKGQGSEFI
                     FELELSQTDNVQPASQSEQEKDWSTVQDLQARFLMAEDHALNQLVTTEMLKGRFPSLQ
                     IDVAETGRQAISLFEDHNYDLVLMDVQMPEMDGHEAARYIRTHFPKPKSEVPILAFTA
                     YATSGEAEKCLEAGMNDYISKPVRATQLISKIMQMLDASEQFSKEHFLHESKVQEHIS
                     FEYLNTVTEDDEELKLRMMRIMLDETPMELKSLEEAAAASQWDNLRAVAHKMKSTMQF
                     LGLEDTLETVKFIEVSARERTHLEQIPEKVRSVVQVCTRALGALQEEIDRIK"
     gene            2383..3351
                     /locus_tag="PK742_07770"
     CDS             2383..3351
                     /locus_tag="PK742_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQU76528.1"
                     /translation="MSSTLISPAQALELVGEGLRTLPFPHAPEGLYDPARYILQLGGK
                     RIRPSLTLLAANIFDADPARFLQPALAMEVFHNFTLVHDDIMDEAPLRRGADTVHIKW
                     DLPTAILSGDLLLIEAYKLLGQAPAHTLPAVLQMFNRTAVEVCEGQQLDMDFEKQTDV
                     TLAEYIEMVRLKTSVLLGCSLATGALCAGASEADTDALYIFGEQLGIAFQIMDDVLDA
                     FPEGDTFGKKAGGDIERGKKTFLYAKAMESMKAEDRDKLLSHYGKPGAGHVDFVLDFF
                     ISNGIKNAATQAMEAHFQRSLQALGTVNGPTQVLHQLAQAVYERTF"
     gene            complement(3353..3988)
                     /locus_tag="PK742_07775"
     CDS             complement(3353..3988)
                     /locus_tag="PK742_07775"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQU76529.1"
                     /translation="MSDSTYFPDILQRAAAAGLPGPEAHRIMAPQYRVVKPFREEDFP
                     GSRKASVLALLYAKNEAVQVVLTRRPDYEGVHSAQISFPGGQSETHDEDHWQTALRET
                     EEEIGVPASIIRHLGDLSPIYIPPSNFFVRPYLGWLDNIPEFHPDPEEVAAVIEMPVL
                     TLLNESAKTTMRIERPDFSAEVPCYRFDEHRIWGATAIILAELEMLLRSVQ"
     gene            4045..7035
                     /locus_tag="PK742_07780"
     CDS             4045..7035
                     /locus_tag="PK742_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU76530.1"
                     /translation="MKASVFGTLLLVSQLSIAQDWAFIPNMGQWDLPVLAETAGSGAK
                     LYLEANALTWQFEDRSDIHRVKEGEIALKDAVYRSHVYRMHFQGADPSAQITPVDHSM
                     QKTYYNFFHGNDHSRWKGGVHGYGEMKYTGLYPGIDLRVYSSGGNFKYDLEISAGADP
                     GIIRLEYDGADSLYLFQDELHISTTLAHIVEHKPVAWQIIKEKKVSVSCNYILDDNIL
                     SFHFPEGYDPLYPIIIDPSTLIFGSYSGSTSDNWGYSATYDSEGNLYGAGIVFGTGYP
                     TVTGSFQTTYAGGEGGSFACDVSISKFTADGTDLVYSTYLGGSKNELPHSLAVDSSGQ
                     LIVYGTTGSDDFPMGEDAYDNSFGGGDNATVTYVVQFSEGTDIYLTKFTADGTDIIGS
                     TYLGGNENDGMNLGDIAFNYGDHARGEVVTNTAGDVYVASCTYSSNFPVSPGAAIPAL
                     QGGQDGIVAHFNADLSSLVWSTYLGGSIDDGAYSIRLAPDGTFIVGGSTNSNDFPVTA
                     GGLNNSFQGGSMDGFLVRLNATGTSILEGTYIGTDSYDQTFIVEVDEENNVYCTGQTS
                     GSYPVTAGVYSNPGSSQYISKLEPDLSALVFSTVFGSGSATVNISPTAMLVDQCESVY
                     VAGWGGTVNSGWNPATGSVSGMPVTADAFSTTTTGSDFYFIVFKKDLTDLLYATFYGS
                     PVANDHVDGGTSRFDKKGVIYEAVCAGCGGLDDFPTTPDAWSTTNNSTNCNLGVIKYE
                     FIYLGPSSGFSVSPDEGCAPLNASFTNSSLAATSYFWDFGDGTTSEESDPDHVYTEPG
                     TYTIMLIALDPESCIPEDTVYQTVEVYGYPEALFDMTVDPFSPIAYNFTDQSVDAATW
                     FWTFGDGSSSTTQNTFHIYEEPGVYTVCLFIENANGCPDTICQELTIDAVSQLVVPNA
                     FSPNGDGYNDIFLPFNFSLIGFELKIWNRWGELVFESNDPNIGWDGTYKGIDQEMDTY
                     VYTVGGLGEDGQDYFFKGNITLVR"
BASE COUNT         1706 a         1774 c         1761 g         1905 t
ORIGIN      
        1 cctgtccgga catcatgatc acatcctgtc ccggattggc ccgcaggatc ttgcgaagta
       61 tttccaggcc attggcagca tctgtgcgaa taccatccag gtagtaatcc agtaccacta
      121 cgtcaggatt ttcctggatt cgctccagac aagcctctcc gctggagaaa atattgatct
      181 ccagatcata atcagatttt ttttccaggt ggttcttgag catcaccaga tgcttcgtgt
      241 catcatccac cagaaatacc ctgtaacgct tcttcttcgt ggtgtccata ggcggcttta
      301 tttgatcctg tctatttctt cctgcagcgc ccccagcgcc cgggtacaaa cttgcacaac
      361 tgaacggact ttttcaggta tctgctccag atgtgttctt tcacgggcag aaacctcaat
      421 aaacttaacc gtttccagtg tatcctccaa acccaggaat tgcatggtgc ttttcatttt
      481 atgggccacc gcacgaaggt tatcccactg agaggcagct gcggcctcct ccagactctt
      541 aagttccatt ggagtttcat ccagcatgat gcgcatcatt ctcagcttca gctcttcatc
      601 gtcttcggtt accgtattca gatactcgaa actgatgtgt tcctgcacct tgctttcatg
      661 cagaaaatgt tctttggaaa attgttctga agcatcgagc atctgcatga tcttgctgat
      721 gagctgagtg gctctgacag gtttggaaat gtaatcgttc attcctgcct ccaggcattt
      781 ttcagcttct cccgaggtag cataggctgt aaatgccagt attggaacct ctgatttggg
      841 tttaggaaaa tgcgttctga tataccttgc ggcttcatgt ccatccatct caggcatctg
      901 cacatccatc agcaccaggt catagttgtg gtcttcgaac agactgatgg cctgcctgcc
      961 ggtctcggcc acatcgatct gaagtgacgg aaaccttccc ttcagcatct cagtagtaac
     1021 cagctggttc aatgcatgat cttctgccat cagaaaacgt gcctgcagat cctgtaccgt
     1081 actccagtct ttctcctgct cagactgaga tgcaggttgt acattatcag tctgcgataa
     1141 ctccagttca aaaatgaatt cagacccctg cccttttctg ctgcgcacca tgatggcacc
     1201 attgagcatg tctatcagtt tcttgacaat ggccagcccc aggcctgtgc ctccatacaa
     1261 tctcgtggtg tctgtactgg cctgggtaaa gctctcaaag atgtcctgca gtttctcctg
     1321 ttcaatgcca atacctgtgt cttttacgct gaagaataac ctggctttgt gatccagttt
     1381 atcgatcagc cggatggtaa cgcgaacctc tccttcaggt gtgaatttga tggcattact
     1441 gatcaggttg agcaggatct ggttgatgcg caaagggtct ccctgcaggt tgggcggtat
     1501 ggacggatcc atatccagca ccagctccag acctttctct tctgccttga aacggatggt
     1561 actgatgatg gcttccaccg tttgctgcag actgaacggt ctgttgtcca gctccatttt
     1621 tcccgcttct atcttgctga aatccagtat gtcattgatg atctgcaaca gcgaatctac
     1681 cgctgtctgc ataccatcca gatattcatg ctgcttgggt tccagattgg tcttgttaag
     1741 taactgaagc attccgccaa tggcattcat cggggtgcgg atctcatgac tcatattggc
     1801 caggaactct gccttgaatt tattggcccg ctctgccagg tcttttgccc gctgcaattc
     1861 aaaggtcttc ttgcgcaggg tgatgtcgcg gatgataccc tgaaaaccta cgatctcatg
     1921 attctggtct cttcgcacgg aactgttcac caggcagtca atgagctccc cgttcttgcg
     1981 tttgagctgc acttcatagt ctcgaacttc ccctttatcg atcagctgct gaagcaggtg
     2041 cctgcggtca tccggatggg cgtagatggc agagaccgat agctgcatca gttccttgta
     2101 tgaatagccc agcatttcca gcagcgcccg gttgcaaccg gtaaaggtgc cgtccagggt
     2161 gctcagatag atggcgtctg tcagctggtc gatgagcatg cggtacttct cctcactgcg
     2221 gtacagatca gactcgatag acttccggtg tacgaagtct ctggcaagca attcacgcag
     2281 tttctggatc aacaggttct cctccatcgc agcgtgcaaa ttagtcattt tcgcaggatg
     2341 tactacaggg tcaggtaggc cgtcaatgtg taattttgct gcatgtctag tactttgata
     2401 tcacctgcac aggctctgga acttgttggc gaaggattac gcaccttgcc ttttccgcac
     2461 gcacctgagg gtctttatga tcctgccagg tatatccttc agcttggcgg taagcgcatc
     2521 cggccttcgc tcactctgct ggcggccaat atctttgatg ctgacccggc caggttcctg
     2581 caacctgcac tggccatgga ggtcttccat aattttaccc tggttcacga tgacatcatg
     2641 gatgaggctc ccctgcgaag aggtgctgac accgtacata tcaaatggga tctgcccacc
     2701 gccatcctga gcggagacct gttgctgata gaagcatata aactgctggg gcaggctccc
     2761 gcccatacat taccggctgt actgcaaatg ttcaaccgca cagctgtgga agtttgtgaa
     2821 ggacagcaac tcgatatgga ttttgaaaaa cagaccgatg ttaccctggc agaatatatt
     2881 gaaatggtga ggctcaagac cagtgtgctt ctgggatgca gtctggcaac cggagcgctc
     2941 tgtgccggtg catctgaagc agacactgac gccttgtaca ttttcggaga acaactgggt
     3001 atcgctttcc agatcatgga tgatgtgctg gatgcgtttc cggaaggaga tactttcggc
     3061 aagaaagccg gtggtgatat tgagcgggga aaaaagacct ttctgtatgc aaaagctatg
     3121 gagagcatga aagcggaaga ccgagacaaa ttgctgtcgc actatggaaa accgggtgcc
     3181 ggacacgtgg atttcgtcct ggattttttc atttccaatg gcatcaaaaa cgccgccact
     3241 caagctatgg aagcccactt tcagaggtca ttgcaagccc ttggaacggt gaacggacca
     3301 acacaggtgc tgcaccagct ggcacaggct gtgtatgaac ggaccttttg atctactgca
     3361 cactgcgcaa caacatctcc agttcagcca gtatgatggc agtagcaccc cagatcctgt
     3421 gttcatcaaa ccggtagcat ggcacctctg cgctgaaatc tggtcgttca atgcgcatgg
     3481 tcgtttttgc tgattcattc agcagggtca gtaccggcat ctcaatgaca gctgccactt
     3541 cttccgggtc gggatgaaac tcaggtatat tatccagcca tcccaggtaa ggacgaacaa
     3601 aaaaattact gggtggaata tagatcggcg acagatcacc aagatgccgg atgatgcttg
     3661 caggaacacc gatctcttct tccgtctccc gcagggctgt ctgccagtga tcttcatcat
     3721 gcgtttctga ctgacctccc ggaaaactga tctgcgcact atgtacccct tcataatccg
     3781 gtcggcgggt cagcactacc tgcactgctt cattcttcgc atacagcaat gccagcacac
     3841 tggcttttct tgaccccgga aaatcttctt ctctgaaagg cttcacgaca cggtactgtg
     3901 gcgccatgat gcgatgtgcc tcagggcccg gtaagccggc tgccgctgct ctttgcaaaa
     3961 tatcaggaaa gtaggtggaa tctgacatgg atatgcctgt tctttcggct caaaataagg
     4021 cattttcgta cattcgggaa agatatgaaa gcatctgtct ttggcaccct gctgctggtg
     4081 tctcagttga gcatagccca ggattgggcc ttcatcccca acatggggca atgggatctg
     4141 ccggtacttg cagaaactgc cggaagcggg gcaaagttat atctggaggc caatgccctg
     4201 acctggcagt ttgaagacag aagtgatata caccgggtca aggaaggcga aatagctctg
     4261 aaagatgcgg tctatcgttc gcacgtgtac aggatgcatt tccagggagc agatccgtct
     4321 gctcagatca ctccggtcga tcattccatg cagaagactt attataattt ctttcatgga
     4381 aatgaccatt cccgatggaa aggtggtgta catggatatg gcgaaatgaa atataccggt
     4441 ttgtatccgg gtatcgattt gcgggtgtac agcagcggcg gcaacttcaa atatgatctg
     4501 gagatcagcg caggtgcgga ccccggcatc atacgtctgg aatacgatgg cgcagattct
     4561 ttatatctgt ttcaggatga actccacata agcactacac ttgcacatat agtggaacac
     4621 aaaccggtgg catggcagat cataaaagaa aaaaaggtat ctgtttcctg taactatatt
     4681 ttagacgaca atattctgtc cttccatttt cccgagggat atgatccctt gtacccgatc
     4741 atcattgatc cgtcaacact catttttggc agttactccg gttctacttc tgacaactgg
     4801 ggatacagcg ccacctacga cagcgaaggg aatctgtatg gggcagggat cgttttcggc
     4861 acaggatatc ctacggtaac aggctccttt cagaccactt atgcaggtgg cgaaggagga
     4921 tcgtttgcat gcgacgtaag catctccaag tttaccgcag atggtacgga cctggtttat
     4981 tcaacttacc tgggtggcag caagaatgag ttgccacaca gcctggcagt ggacagcagc
     5041 ggtcagctta tcgtttacgg cacaacaggt tctgacgatt ttcccatggg tgaagatgcg
     5101 tatgacaata gttttggtgg tggcgataat gcaactgtta cctatgtggt tcagttttca
     5161 gaaggtaccg atatctacct gaccaaattc actgccgatg gcactgacat cattggttct
     5221 acttatctgg gtggcaatga aaatgacggc atgaacctgg gagatatcgc attcaactac
     5281 ggcgaccatg cacgtggaga agtagtgacc aatactgcag gagatgtcta tgtagccagt
     5341 tgtacataca gcagcaattt ccccgtttct ccgggtgcag ccatacccgc cttacagggt
     5401 ggtcaggatg gtatcgtggc acatttcaat gcagacctca gcagtctggt atggtccacc
     5461 tatctgggag ggtctattga tgacggagcg tattccatac gcctggcacc cgacggcacc
     5521 ttcattgttg gaggctcaac gaacagcaat gattttcctg taacagcagg tggcctgaac
     5581 aacagttttc agggtggttc gatggatggt ttcctcgtgc gcctgaatgc taccggaaca
     5641 agcatcctgg agggtaccta cattggcacc gacagttatg atcagacctt tattgtggag
     5701 gttgatgaag aaaacaatgt gtattgtacc gggcagactt caggatctta tccggtaacc
     5761 gcgggcgtgt acagcaaccc tggaagttca caatacatca gcaagctgga accggatctg
     5821 agtgcactgg tattttcgac agtttttggc agcggctcgg caactgtaaa tatatctcct
     5881 actgccatgt tggtggacca gtgtgaaagt gtttacgtgg caggatgggg cggcacggtg
     5941 aattctggct ggaatcctgc aacgggaagt gttagtggca tgccggtcac agctgatgca
     6001 ttcagtacca ctaccacagg cagtgatttc tatttcattg tattcaaaaa ggatcttacc
     6061 gatctgttat atgccacttt ctatggcagc cctgttgcca acgatcatgt ggacggtggc
     6121 accagtcgtt tcgataaaaa gggagtcatc tatgaggcgg tatgtgcagg atgcggaggt
     6181 ttagatgatt tccccaccac acccgatgcc tggagcacca ccaacaacag caccaactgc
     6241 aatctgggcg taataaaata cgaattcatc tacctgggtc catcctcagg attttctgtc
     6301 agtcctgatg agggttgtgc acccctgaat gcttccttta ccaacagcag tctggcagca
     6361 accagctatt tctgggactt tggcgatgga actacttcag aggaatctga tcctgatcat
     6421 gtctatacag aacccggcac ctataccatc atgctgatag ctctggatcc ggagtcctgt
     6481 attccggaag acaccgtata tcagacagtg gaagtatatg gctacccgga ggcattgttc
     6541 gacatgacag ttgacccttt cagtccgatt gcctataatt tcaccgacca gagtgttgat
     6601 gccgctacct ggttctggac cttcggagat ggcagttcat ccactaccca gaataccttt
     6661 catatatatg aagaacccgg agtatatact gtttgtctgt tcattgaaaa tgccaacggt
     6721 tgcccggata ccatctgtca ggaactgacc atagatgctg tctcacaatt ggtagtaccc
     6781 aatgcattct ctccgaacgg tgatggctac aatgacatct tcctgccatt caatttcagt
     6841 ctgatcggtt tcgagctgaa gatctggaac aggtggggag agctggtctt tgaatcgaac
     6901 gatcccaaca tcgggtggga cggcacctac aagggcattg accaggagat ggacacttat
     6961 gtttataccg taggtggtct gggagaagac ggacaggatt acttcttcaa agggaatatc
     7021 acactggtga ggtaaagggt tccgggttat tggtctttgg tacctggttt ttggttcttc
     7081 tacataataa tgagactgtt caaaaaactg tgtcacttaa atttgagtga tacatgaaaa
     7141 aggaga
//
LOCUS       DAOMAX010000129         7107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1607_length_7107_cov_4.379183, whole genome shotgun sequence.
ACCESSION   DAOMAX010000129 DAOMAX010000000
VERSION     DAOMAX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_7107_cov_4.379183"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK742_07785"
     CDS             complement(<1..336)
                     /locus_tag="PK742_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HQU76531.1"
                     /translation="MDMHAIDFQPLEINRISQLISRRIPLVLSQEAEAAIRHTREYLD
                     VVLDRRPEPVYGVNTGFGSLCKVRISPDDLESLQRNLILSHACGTGPELEAETIRYLL
                     FLKIQSLSYG"
     gene            complement(366..1793)
                     /gene="rodA"
                     /locus_tag="PK742_07790"
     CDS             complement(366..1793)
                     /gene="rodA"
                     /locus_tag="PK742_07790"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQU76532.1"
                     /translation="MREPQNIIQRLDKPMLVLYLLMLLVGLMTVFAVNYNEESSRFFN
                     LSQTHGRQMTWMFISLVLGFSMLTFNSNFFTKFSIPFYIIIILLLLVTLAVAREINGA
                     RSWLQIGPAQFQPAEFAKTATALMLAKYISSLSNAVKTFRQKMIAAGIILLPMAIIIL
                     QEDMGSALVYTSLSLVLYREGFKVREVIIALLFGAGFILSMLVSVQVLLWVLSGVVLL
                     IAAFSSGRLWRKDKPRYLRYWLLFLLSVASFAAGYYLPALRIYLIVLAVLFMAGVWMA
                     LRPVAYMRITPVFWLYLALTGFTLYGSSFVMNELLESHQSERVLTLLGLSDDPDANYN
                     VTQSKMTIGSGGFAGKGYLEGTLTQLKHVPEQSTDFIFCTIGEELGFLGTFFFLAIYL
                     AFILRIIHLAERQRSPFTRIYCYSVASIFFIQVMINVGMTIGLLPVIGIPLPFISYGG
                     SSVLAFSIMVFIVLRLDADRLLILR"
     gene            complement(1790..3700)
                     /gene="mrdA"
                     /locus_tag="PK742_07795"
     CDS             complement(1790..3700)
                     /gene="mrdA"
                     /locus_tag="PK742_07795"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQU76533.1"
                     /translation="MTIHDQYKNRTWVIRGLIAGIFLLFMIRLLVLQVINKDYAELAK
                     DRTVRKVTVYPDRGQMYDRNGKLIVHNKPIYNLMVIPSQVKDMDTLRFCRMLGIDTIY
                     FQEKLAEAKRYSPLHASVFLPQIEGEEFARMEEYMYEFPGFFPEIRTMRTYPYANAAH
                     LLGYVTEVDSSDIKRSNGYYKGGDYIGKSGFEKSYEEVLRGKKGYRYMVVDAHNRLMG
                     SLANGEKDEAAEAGKNVTLTIDIDLQRFAESLMQGKKGAVVAIEPKTGEILALVSSPA
                     YDPNLLTGSSRTKNYGTLVMDEGRPLNNRALTGYYPPGSQFKALMALVALQEGTLVAD
                     QGYPCGGGYRMSGHTVGCHNHVTPSNVVMGIQHSCNAYFCFVLKHFIDDHNANAPEGL
                     EKLKDYLASFGIGVPTGIDLPGEKGGNVPDPEDYDRIYGKGRWKSSNFITLGIGQDQM
                     IVTPLQNANMMATIANRGYYISPHVFKYADGEDSLLQALSVKHYTKVDEQYFDVVTEG
                     LSMVVKAGTARVARIDSIEVCGKTGTAENPHGKDHSQFSGFAPRENPQIAVAVLVENS
                     GFGATWAAPIASLVMEYYLKGSISPSRQYLLDRMRNTNLIGTPTQPVAPEPEEDRNDP
                     DVLVNDTTAIKP"
     gene            complement(3697..4224)
                     /locus_tag="PK742_07800"
     CDS             complement(3697..4224)
                     /locus_tag="PK742_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76534.1"
                     /translation="MQVTRWIWRVIGWIALTAFQVLVLTHLNIGQYVHPYVFPMFIML
                     LPFATPRWLVLILAFISGLIVDMFNNTPGMHASAMLVLAFLRPAVTRLLTPITGYEGI
                     QAPSMGRLGFIWFLLYALLLTSIHHLVYFQLEVLSFSNLGYTLLNTAASVLFSVFLMV
                     NFAFIFSGNRQGERT"
     gene            complement(4211..5044)
                     /gene="mreC"
                     /locus_tag="PK742_07805"
     CDS             complement(4211..5044)
                     /gene="mreC"
                     /locus_tag="PK742_07805"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQU76535.1"
                     /translation="MRSFIQFLLRYHVFFLFLLLELISLWFLFSNNAYQRTFFVHSAN
                     RMTGNVLNTYGNLTKFFYLSEVNDSLLAENALLREAMMQQATADSGGRISMLDEHGEM
                     LYSCMPAKVINNTFTLPNNYITLDKGSEDGIVKDMGVITSNGLVGIVKDVSPHFSVVL
                     SVLHSHFHTRVAVKKNNVQGRLVWNGEDPTVVKIVDVSEPGHLEPGDTIVTTGYSAAF
                     PPGYPVGVLKSYGKETGSNFYTLEVELTTAFSRLHYVYVVDYLLKEEQTNLEEESHAG
                     N"
     gene            complement(5058..6083)
                     /locus_tag="PK742_07810"
     CDS             complement(5058..6083)
                     /locus_tag="PK742_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQU76536.1"
                     /translation="MGLFNFLSQDIAIDLGTANTLIIAHDKVMVDEPSIVAIDRKTDK
                     VIAVGKRAMQMHEKTHENIKTVRPLKDGVIADFQAAENMMRGMISMIFPKNRIFKPSL
                     VMVICIPSGITEVEKRAVKDSAEQAGAKEVYLIQEPMAAALGIGLDVQEPVGHMIIDI
                     GGGTTEIAVVALSGIVCDQSIRVAGDEFTADIVDYMRRQHNLLIGERTAEAVKINVGS
                     AMAELDDAPEDYQVSGRDLMTGIPKQLVVTYSEIAHALDKSISKVEEAILKALETTPP
                     ELASDIQREGIHLTGGGALLRGLDKRISQRTKLTVHVADDPLRAVVRGTGIALKNREH
                     YQALFIG"
     gene            complement(6127..>7107)
                     /locus_tag="PK742_07815"
     CDS             complement(6127..>7107)
                     /locus_tag="PK742_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HQU76537.1"
                     /translation="RYDTAIASWMQGGGQNMLDSFGPKASLRYGENPHQDAAFYGDLS
                     EVFHKIQGKEISYNNLVDIESAVYLMEDLRGFAGEQAAFAILKHTNACGVALADSLSE
                     AWQKALAADPVSAFGGVLIANKEVDAATAAMIHGLFFEVIIAPSYAADALTILAGKKN
                     RIILEQKRFDFPAEQFRSLLHGVLAMDRNNHLTASSALKLVTEARPDAAQTDDLLFAE
                     LVVKHLKSNGIALVKNRQLISMGTGQTSRVDALNQAIAKAKAFGFETEGSVMASEAFF
                     PFPDCVEIAYHAGIKAVIQPGGSIKDQDSIDYCNAHGMAMVLTGVRHFKH"
BASE COUNT         1823 a         1870 c         1643 g         1771 t
ORIGIN      
        1 accataagac aatgactgta tcttcagaaa aagcaggtat ctgatggtct ctgcttccag
       61 ctccgggccg gtaccgcagg catgagataa aataagatta cgctgtaagg attccagatc
      121 atcaggtgaa atacgaacct tacataatga tccgaaaccg gtgttcacac catagaccgg
      181 ttcaggtctt ctgtcaagca ccacatccag atactctctt gtgtgcctga tggcagcttc
      241 tgcttcctga ctcagcacca gcggaatgcg ccggcttatc agttggctga tacggtttat
      301 ctccaggggt tgaaaatcaa tggcatgcat atccatagca ggcaaatttg caaaaattac
      361 acttgtcagc ggagtatcag cagtcggtca gcatccagcc ggagcacaat gaataccatg
      421 atgctgaaag caagtaccga agaaccaccg taagagatga aaggtaaagg aattccgatt
      481 acaggcagca gcccaatggt catacctaca ttgatcatga cctgaataaa gaaaatactg
      541 gcaactgaat aacagtatat ccgggtgaat ggagagcgct gccgttctgc caggtgaatg
      601 atgcgcagaa taaaagccag gtagatggcc agaaagaaga aggtgcccag aaagcccagt
      661 tcttctccga tggtgcagaa aatgaaatca gtgctttgct ccggaacgtg tttgagctgg
      721 gtcagggtgc cttccagata tccttttccg gcaaacccac cggagccaat ggtcatcttg
      781 ctttgcgtca cgttataatt ggcatccgga tcatcactca gccccagcag cgtgagtacc
      841 ctttctgact gatgagactc cagcaattcg ttcatcacga aactggaccc gtacagggtg
      901 aatcctgtca gcgccagata taaccagaat acaggagtga tgcgcatata ggctaccggg
      961 cgaagtgcca tccaaactcc agccatgaaa agcaccgcca gaacgatcag atagatgcga
     1021 agggccggca gataataacc ggctgcaaag gatgctactg acaataagaa caacaaccaa
     1081 tatcgaagat acctgggctt gtcttttctc cacaacctgc cggaactgaa tgctgctatc
     1141 agcagcacga ccccggaaag cacccataat aatacctgta cggaaaccag catagacagg
     1201 atgaaaccgg ctccgaacaa cagggcaatg atcacctctc ttaccttgaa cccttctctg
     1261 tacaacacaa gagataatga agtatagacc agagctgaac ccatatcttc ctgcagaatg
     1321 ataatggcca tgggaagcaa tatgatacct gcggcgatca ttttctgccg gaatgttttt
     1381 accgcattgc tcaggctgct gatgtatttg gccagcatca gagctgtagc tgtcttggca
     1441 aattcggccg gctgaaactg cgccggacca atctgcaacc agctgcgggc accattgatc
     1501 tctctggcaa ctgccagcgt taccagtaac aataaaatta tgatgatata aaatggaatg
     1561 gaaaacttgg taaaaaagtt gctgttgaaa gtgagcatac taaagcccag aaccagcgag
     1621 atgaacatcc aggtcatctg tcttccatgc gtctgtgaca gattgaaaaa acgactggat
     1681 tcctcattgt agttgacagc aaatacggtc atcaggccaa ccagcagcat gagcagatac
     1741 agcaccagca tgggcttgtc cagtctctgt atgatattct gaggctctct catggcttga
     1801 tggcggtagt atcgttgacc agcacatcag gatcgttacg gtcttcttcc ggctccggtg
     1861 ctactggttg tgtgggggtt cctatcagat tggtgttacg cattctgtcc agcagatact
     1921 gacgggatgg tgagatactg cctttcagat aatattccat gaccagactg gcgatcggtg
     1981 ccgcccaggt tgctccgaaa ccggaatttt caaccagcac tgcaacagcg atctgaggat
     2041 tctctctggg tgcgaagcca ctgaactgac tgtgatcctt cccatgcggg ttctcagcag
     2101 ttcctgtctt cccgcaaact tcaatactgt ctatcctggc aacccgtgca gttcctgctt
     2161 tgaccaccat actgagccct tcggtcacta catcaaaata ctgctcatct accttggtat
     2221 aatgcttcac agagagcgcc tgcaacaggc tatcctcacc atcggcatat ttgaacacat
     2281 gcggagaaat ataataccca cggttggcta ttgtggccat catattagca ttttgtaatg
     2341 gggtcacgat catctgatcc tgcccgatac ccagggtgat gaagttggat gacttccacc
     2401 ggcccttacc gtagatgcgg tcatagtctt ccgggtcagg tacattgcct cctttctcgc
     2461 ccggaaggtc aattcctgta ggtactccga tgccgaaact ggccagataa tctttgagct
     2521 tttccagccc ttcgggtgca ttggcattgt ggtcatctat gaaatgcttc agcacgaaac
     2581 agaaataggc attgcaggaa tgctggatgc ccatgaccac attagaaggt gtaacatggt
     2641 tgtgacaacc cacggtatgt ccgctcatac gataacctcc gccgcaggga tagccctgat
     2701 ctgcaaccag agtgccctcc tgtaacgcta ccaaagccat caatgctttg aactgtgaac
     2761 caggcggata gtaacctgta agtgcacgat tattgagtgg cctgccttca tccatgacca
     2821 gggtaccgta attttttgtc ctggaactgc cggtcagcag attggggtca taggccggac
     2881 tgcttaccag tgccaggatc tcacccgttt tcggttcaat ggctactacc gcacccttct
     2941 tcccctgcat gagtgattct gcaaatcttt gcagatcaat atcaatggtc agggtaacat
     3001 tcttaccggc ttcagctgct tcatcctttt cgccattggc caggctgccc atgagtctgt
     3061 tatgggcatc cacgaccata tacctgtaac ctttcttacc gcgaagtact tcttcatagg
     3121 atttctcgaa accactttta ccaatataat cacctccttt gtagtagccg ttggaacgtt
     3181 tgatatcgct gctgtccacc tcggtcacat accccagcag gtgggccgca tttgcataag
     3241 ggtaggtgcg catggtgcgt atctccggaa agaaaccggg aaactcatac atgtattctt
     3301 ccatcctggc aaattcttca ccctctatct gcggaagaaa tacagaagca tgcagcggcg
     3361 aatatcgctt cgcttctgcc agcttctcct gaaaatagat ggtatctata cccagcatcc
     3421 tgcaaaacct cagggtatcc atatctttca cctggctggg aatgaccatc aggttgtaaa
     3481 taggtttgtt gtgtacgatc agcttgccat tgcggtcata catctgtccg cgatcgggat
     3541 acacggtaac cttcctgacg gtcctgtctt tggccagttc tgcatagtct ttgttaatga
     3601 cctgcagcac aagtaacctg atcatgaaca acagaaatat accggctatc agtcctctga
     3661 tgacccaggt gcggttctta tactgatcat gtatggtcat gtgcgttctc cctgtctgtt
     3721 gccgctgaaa atgaaagcaa agttgaccat taaaaataca gagaacagta cactggcagc
     3781 ggtgttcagc agggtgtatc ccagattact gaatgagagc acctccagct gaaaatacac
     3841 cagatggtga atggaggtga gcaacagggc gtataacaga aaccagatga atccaagccg
     3901 ccccatagaa ggcgcctgaa taccttcata acctgtgatg ggtgtgagca gcctggttac
     3961 agccgggcgc aggaaagcca gcaccagcat ggcagaggca tgcataccgg gggtgttgtt
     4021 gaacatgtca acgatcagac ctgaaatgaa agccagtatc agtaccagcc accgtggggt
     4081 ggcaaaaggc agcagcatga tgaacatggg aaatacatac ggatgcacat actgtccaat
     4141 attcagatga gtaagcacca gcacctgaaa ggctgtcaga gcgatccagc ctatgacacg
     4201 ccatatccac ctagttacct gcatgactct cctcctccag gtttgtttgc tcttctttca
     4261 gcagataatc cactacatag acatagtgca ggcgactgaa cgcagttgtc aattccactt
     4321 ccagggtata gaaattactg cctgtttctt ttccatatga cttcagcact ccaacaggat
     4381 aaccgggtgg gaaagcagca gaatatcctg tggtcacaat ggtgtctccc ggctccaggt
     4441 gcccgggttc agacacgtcc actattttga caactgtagg atcctctcca ttccatacca
     4501 gtcgtccctg cacattgttc ttcttgacag cgaccctcgt atggaaatga ctgtgcaaca
     4561 cggatagtac cacgctgaaa tgcggcgata cgtcctttac gataccgacc agtccgttgg
     4621 aggtgatcac tcccatatct tttactatgc catcttcact tcccttgtcc agtgtgatat
     4681 agttgttggg caatgtgaat gtattgttga tcaccttcgc aggcatacag gagtacagca
     4741 tctctccatg ctcatccagc atgctgatgc gcccgcctga atctgccgtg gcctgctgca
     4801 tcatggcttc ccgcaaaagg gcattctctg ccagcaggct gtcattaacc tcagacaaat
     4861 agaaaaactt cgtaaggttg ccgtaagtat tcagcacatt gccggtcata cggttggctg
     4921 aatgcacaaa aaaggtgcgc tgataagcgt tattgctgaa taggaaccag agagatatca
     4981 actccagtaa aagaaacagg aagaacacat ggtatctgag caggaactgt atgaaactgc
     5041 gcatccggct ctgtcagtta gccgatgaac aatgcctgat aatgctctct gttcttaagc
     5101 gcaatgccgg ttccgcgaac aaccgcacgc aacggatcat ctgccacatg aacggtcagt
     5161 ttggtacgtt gagagatacg cttatccaaa ccccgcagca aggcacctcc accggtcagg
     5221 tgaatgcctt cacgttgtat atcactggcc agttccggcg gagtggtttc cagtgctttc
     5281 agaatggctt cttctacttt agaaatggac ttgtccagcg catgggcgat ctccgaataa
     5341 gtgaccacca gctgtttcgg aatcccggtc atcaggtcac gcccgctcac ctggtaatct
     5401 tccggtgcgt catccagttc tgccatggca gagcctacat tgatcttcac agcttcagct
     5461 gtacgctcgc ctatcagcag gttatgctgc ctgcgcatat agtccacaat atctgcagtg
     5521 aattcatcgc ccgccacacg aatgctctga tcacatacaa taccagacaa tgctaccact
     5581 gcgatctccg tagtacctcc gccaatatca atgatcatat gcccgaccgg ttcctgcacg
     5641 tccagaccga tacccagtgc cgctgccata ggttcctgga tcagataaac ttccttggca
     5701 ccggcctgct ctgctgaatc cttgacggca cgcttttcca cttctgtgat accggaaggg
     5761 atgcagatca ccatcaccag tgatggtttg aatatcctgt tcttggggaa gatcatgctg
     5821 atcatacccc gcatcatgtt ctcggctgcc tgaaaatctg ctatcacccc atccttgagc
     5881 ggacgaacgg tcttgatgtt ctcgtgcgtc ttttcgtgca tctgcatggc ccgtttgccc
     5941 accgctatga ccttatcagt cttcctgtcg atggccacaa tggaaggctc atctaccatg
     6001 accttgtcat gcgcaatgat gagggtgttg gcggtaccaa ggtcgatggc tatgtcttgc
     6061 gacaaaaaat tgaataatcc catgcaagta tttctttatg cccgaaaggc agcaatttac
     6121 gtttttttag tgtttgaaat gcctgacacc tgtcagtacc atggccattc catgggcatt
     6181 gcagtaatca atggaatcct ggtccttgat agagccgccc ggttgaatga ccgctttgat
     6241 tcctgcatga tatgctatct ccacacaatc cgggaaagga aaaaatgcct cactggccat
     6301 gaccgatccc tcagtttcaa agccaaatgc cttggctttg gcaatggcct ggttcagggc
     6361 atcaacccgg ctggtctgtc cggttcccat gcttatcagc tgtctgttct ttacaagtgc
     6421 aattccgttt gacttgagat gcttcactac cagttcagca aacaacaggt catctgtttg
     6481 cgctgcatct ggtcttgctt ctgttacaag cttcagtgct gatgacgcgg tcaggtggtt
     6541 gttcctgtcc atggccagta caccatgcag caggctgcgg aattgttcag caggaaaatc
     6601 aaacctcttc tgctccagaa tgatccggtt ctttttaccg gccaggatgg tcagcgcatc
     6661 tgcagcatac gatggtgcaa tgatgacctc aaagaacaac ccatggatca tggctgcagt
     6721 ggcagcatct acctctttgt tggcaataag caccccgccg aatgctgata ccggatcagc
     6781 tgccagcgct ttctgccatg cttcagacaa gctatcggca agcgctaccc cgcaggcatt
     6841 ggtatgtttg agaatggcaa atgccgcctg ctcaccagca aatccccgca agtcttccat
     6901 caggtaaacg gcagactcta tatctactaa attattataa gatatttcct taccctgtat
     6961 cttatggaat acttcagaca gatctccata aaaggcagca tcctgatgcg gattctcacc
     7021 gtagcgcagg gaggccttcg gcccgaagct gtccagcatg ttctgcccac cgccctgcat
     7081 ccaggaggca atagcagtat cataccg
//
LOCUS       DAOMAX010000130         7038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1632_length_7038_cov_3.801518, whole genome shotgun sequence.
ACCESSION   DAOMAX010000130 DAOMAX010000000
VERSION     DAOMAX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_7038_cov_3.801518"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..418)
                     /locus_tag="PK742_07820"
     CDS             complement(17..418)
                     /locus_tag="PK742_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76538.1"
                     /translation="MKFAAIDIGSNAIRLLFANVYETKNGPVFYKDELVRVPVRLGED
                     AFLTGKISDAKADLVVKTMKAMKLLMDIHKPVAYKACATSAMRDAQNGPALIKRVHKE
                     AGIDIQLLSGEAEASVIMRYPLKQAVSALSA"
     gene            complement(415..2508)
                     /gene="ppk1"
                     /locus_tag="PK742_07825"
     CDS             complement(415..2508)
                     /gene="ppk1"
                     /locus_tag="PK742_07825"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQU76539.1"
                     /translation="MAKKGKDIKFRYIHRDLSWLSFNERVLQEAEDKRVPLNERVRFL
                     GIFSNNQDEFFRVRVATLRRMAKLEKEQIKAFDIRPSKIIGQIQKKVLVLKNRFDSSY
                     LELLKELEKENIFLLNEKQLDKQQGEAVRDHFNDNVRPRLFPIMLDNLNRLPVLSDTS
                     IYLAVRLTRDGQLPRFSLIEVPDKDPSRFFVLPSQNERLSIILLDDVIRYNLDTIYQI
                     FEYDKIEAYTIKLTRDAELDFDSDLSQSLIELIQKSVKRRVKGEPVRFIYDAEMPKDM
                     LDFLTKKVKLQKDDALIPGARYHNFKDFIKFPDLRRIGLRYRVHPPVLHPDLHPQMSV
                     FQVIRRQDVMLHFPYQTFNHVIDFLREAAITPSVTHIYMTLYRVAKNSSVVLSLINAA
                     RNGKSVTVVMELQARFDEENNIYWANRLQEEGVRVIYGVNKLKVHAKLCLVRESVGNR
                     TLHYASIGTGNFNEETARLYTDIMLMTTRPSVVREVIQVFSFFDNQYDIGAYKNLIMA
                     PLFLRDKMRQLIKREIRNAEAGKPASIFIKVNNLVDKDIIRLLYEASEAGVDIRLIVR
                     SACSLVPEVKGMSSRIRAISILDKFLEHPRIYYFHNDGKEDVYIGSADVMERNIDNRV
                     EVLVRLLDHKIRQQVIDILEIQWSDNMKARNLSQQHLNEYIQPEAHEAPVRSQIAIYE
                     YFKNYLETENPDKSL"
     gene            complement(2629..3159)
                     /locus_tag="PK742_07830"
     CDS             complement(2629..3159)
                     /locus_tag="PK742_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76540.1"
                     /translation="MKKVIFPLAAVLMMTACSKSDLEDEMIFPDQVRNGTFEDIELAT
                     NYWTTIGEGSLGLSSDDQAFQGLHAMTISPIGCFEVDYNEPLNVEEGATYELSFATKI
                     TGYSAVCLAEFYLVLEQDGESILEVNIGQGTALDWDHRVLYVRMDSSSPVYLKIFAGS
                     EMILLDQVQFKRISSL"
     gene            3409..4572
                     /locus_tag="PK742_07835"
     CDS             3409..4572
                     /locus_tag="PK742_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQU76541.1"
                     /translation="MNGKYDLRGVSSGKEEVHEAIKDLSKGLFPLAFCKILPDVAGQD
                     PDHCNIMHADTAGTKTALAYLYWRETGNTGVWEGIAQDALVMNLDDMACVGVTDQFLI
                     SSTIGRNKHLIPGEVIAAVIKGTNRFIENMAEQGIHIHHSGGETADVGDIVRTIDVGI
                     TAFARIPKSSLLVNRIKPGNVIVGLASFGQASYESAYNGGMGSNGLTSARHDVLHHDY
                     SSVYPESFSPETDEAVVYSGSRKLTDPSPVEGIDTGTLILSPTRTYLPVIREILVNHR
                     EAIDGMIHCTGGGQTKVLHFAENVHIIKDNLFPVPPLFRMIQEESRTDWKEMYRVFNM
                     GHRLEIYCSEEAAEGIMKIASSYAIDSRIIGHVTSNQGARVTVAGEDNTYIYS"
     gene            4621..5844
                     /gene="rocD"
                     /locus_tag="PK742_07840"
     CDS             4621..5844
                     /gene="rocD"
                     /locus_tag="PK742_07840"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HQU76542.1"
                     /translation="MQTISSARFIELEERYGAHNYHPLPVVLSRGEGALVWDVEGKRY
                     FDFLSAYSAVNQGHCHPRLVEVMREQAGILTLTSRAFHNNLLGEYEQYITNIFGYDKV
                     LPMNSGVEAVETAIKLCRKWGYQVKGIEPGKARIIVLEDNFHGRTTGVISFSTDPSST
                     TDFGPFMPGYEMVPYNDLPALEAALKDPDIAGLLIEPIQGEAGVVVPDDGYLSAAFEL
                     CRKHNILFMADEIQTGLCRTGKMLACDHENIHPDVLILGKALSGGMMPVSAVLADDPV
                     MLCIQPGEHGSTFGGNPLACRIAMTALDILQEEQLAENADRMGQMLRNALQKMSGNVI
                     RSVRGKGLLNAIVVEPMNGRDAWDFCLMLKENGLLAKPTHGDKIRFAPPLVITEEQML
                     ECIEIIRKTEAQFLA"
     gene            complement(5813..6466)
                     /locus_tag="PK742_07845"
     CDS             complement(5813..6466)
                     /locus_tag="PK742_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU76543.1"
                     /translation="MRVELTGVSKRFNYDWIYRGIDQVFESGQIWAVTGANGSGKSTF
                     LRMVAGMLTPSEGSIRYRLDGHAIDREKIFSHVAVTAPYIDLPEEFTLEENIRMQGKL
                     KPYRGGLDTDAVAEATGLSAHRKKELRFFSSGMKQRLRNTLAILSDASLLIMDEPTSH
                     LDLEGVEWYQQLLLSHASGRLILIGSNMERETTMAGHRLQMSEYKQVVRPGIVPRSS"
     gene            complement(6463..>7038)
                     /gene="lpxA"
                     /locus_tag="PK742_07850"
     CDS             complement(6463..>7038)
                     /gene="lpxA"
                     /locus_tag="PK742_07850"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQU76544.1"
                     /translation="RGENTTVIIGDNTTIRECVTVNRGTSYANTTTIGSNCLLMAYSH
                     VAHDCIIGDHVILANSVNLAGHIEIDDWVILEGMVAVQQFIKIGAHAFIAGGSLVRKD
                     VPPFVKAAREPLAYAGVNSIGLRRRGFSAETIEHIQDIYRTLFVRGFNTTKALGIIEA
                     EFPASAERDQILTFIRTSKGGVMKGFRHLSE"
BASE COUNT         1832 a         1772 c         1597 g         1837 t
ORIGIN      
        1 cgaaggcgat gcgctactac gccgacaacg ccgagaccgc ctgtttcaat gggtaacgca
       61 tgatgacaga agcttcggcc tctccactca gtaactggat atcaatgcca gcctccttat
      121 gtaccctttt gatcagcgca gggccattct gtgcatccct catggcactc gtagcacagg
      181 ccttatatgc aacaggctta tgaatgtcca tgagtaattt catagctttc atggtcttga
      241 ccaccaggtc tgcttttgca tcagatatct ttcctgtcag gaaggcatct tctccaagtc
      301 taacgggcac acgaaccagt tcatctttgt agaaaacagg tccgttctta gtttcataca
      361 cgttcgcaaa cagtaaacga atggcgttgc ttcctatgtc aattgcggca aacttcacag
      421 gctcttatca gggttttcgg tttccagata attcttaaag tattcataaa tggcgatctg
      481 actccgcacc ggtgcctcat gtgcttccgg ctgaatatat tcattcagat gttgctgaga
      541 aagatttcgt gctttcatat tgtcactcca ctggatctcg agaatatcta tcacctgctg
      601 tctgatctta tgatccagca agcgaaccaa tacctccaca cggttatcaa tattgcgctc
      661 catgacatct gcagatccaa tatagacatc ttctttccca tcgttatgga aatagtagat
      721 acgcgggtgt tccagaaatt tatccagaat gctgatggca cgaatgcgac tgctcatccc
      781 ttttacttca ggcaccagtg agcaggcact ccttacgatc aacctgatgt caacaccggc
      841 ttcgcttgcc tcatacaaca gccggatgat atctttatct accaggttat tcaccttgat
      901 gaaaatactg gcaggtttgc cggcttcggc attccttatc tctcttttga tcagttgccg
      961 cattttgtca cggaggaaca gcggtgccat gataagattc ttataggcac caatatcata
     1021 ttggttatca aagaagctga agacctggat cacttccctg accaccgaag gtctggttgt
     1081 catcagcatg atatcagtgt acagtcttgc cgtttcttca ttaaaatttc cagtgccaat
     1141 ggatgcatag tgcagggttc tgttgcccac cgattcacga accaggcaaa gcttggcgtg
     1201 caccttcagt ttgttgacac cgtatatgac ccgcacgcct tcttcctgta gcctgttggc
     1261 ccagtagatg ttgttctctt catcgaagcg tgcctgcaat tccatgacca ccgtaactga
     1321 tttgccgttg cgggccgcat tgatcagaga caataccaca ctgctgttct ttgccacccg
     1381 gtagagtgtc atatagatgt gggtgaccga aggagtgatg gcagcttccc gcagaaaatc
     1441 gatcacatga ttaaaggtct ggtaaggaaa atgcagcatc acatcctgcc tgcgaatgac
     1501 ctgaaaaaca ctcatttgcg gatgcaggtc agggtgcaat accggcggat gcacacggta
     1561 cctgaggcct atgcgccgaa ggtcaggaaa cttgataaag tctttgaagt tatggtaacg
     1621 tgccccggga atcagcgcat catccttctg caatttgacc ttctttgtaa gaaagtccag
     1681 catatccttg ggcatctctg catcataaat aaatctgacc ggctcgccct taacacggcg
     1741 cttcacgctc ttctgaataa gctctatcag actttgcgaa agatcactgt cgaaatccag
     1801 ctctgcatcg cgggtcagtt tgatcgtgta ggcttcgatc ttgtcatatt caaagatctg
     1861 atagatggta tccaggttat agcgtatcac atcatccagc aggatgatgg acaagcgttc
     1921 attctgactg ggcagcacaa agaacctgga aggatcttta tccggcactt cgatcagtga
     1981 gaacctgggc aactgaccat cgcgggtcag cctgacagcg agatagatcg aagtatcact
     2041 tagaacaggt aaccggttca ggttatccag catgatgggg aacagtctcg gacgcacatt
     2101 gtcattgaag tgatctctga cggcttcccc ctgctgcttg tccagctgct tctcattgag
     2161 caggaaaata ttctccttct ccagttcctt tagcagctcc aggtaagagc tgtcaaaacg
     2221 atttttgagt accagaactt tcttctgtat ctggccgatg atcttgcttg ggcggatgtc
     2281 aaaagctttg atctgttctt tctccagttt tgccatccgc ctgagggtag ccacccgcac
     2341 ccgaaagaac tcgtcctggt tattggaaaa gataccaaga aagcgaacac gctcatttag
     2401 cggaaccctt ttgtcttccg cctcctgcag caccctttca ttgaaagaca gccaactcag
     2461 gtcacgatgt atatacctga acttgatatc cttccccttc ttagccatcc cgctaaatta
     2521 cgcgaagcag actggttgaa ataagatgtt gaattaataa ttctttaatt cagtcagggc
     2581 aaaaaaaaag cgggccattg cccgcttgca agtcaatcct ttaccctgtc aaagagaaga
     2641 tatacgcttg aactgaacct ggtcaagcag gatcatttca ctaccggcaa atattttcag
     2701 ataaaccggc gaactgctgt ccatccgcac atacaatacc ctgtgatccc agtcaagagc
     2761 tgtaccttgt ccaatattca cttccaggat actttcacca tcctgctcca gaaccagata
     2821 gaactcagcc agacatacag cagagtatcc ggtgatcttg gtggcaaatg acaattcata
     2881 cgtagctcct tcttctacgt tcaaaggctc attgtagtcc acttcaaagc acccgatcgg
     2941 tgagatcgtc atagcatgca acccctggaa cgcctgatca tctgatgaca gacccaggct
     3001 gccttcgcct atagtggtcc agtaattggt tgccagttca atgtcttcga aggtaccgtt
     3061 gcgtacctgg tcaggaaaga tcatttcatc ttccaggtca gacttggaac aggcggtcat
     3121 catcagaaca gcagcgaggg ggaaaataac ctttttcata tgcagctatt taggattaaa
     3181 aatacacctt ttcaggcgct tgctgtgaca taaactgcta aaaacataga aaggttgcct
     3241 gtaatcaaaa ataatttgtg acatcattaa aattacacaa acaaccacat attgctcaat
     3301 atgataaaat tattataatt atagctaatt aatgtgacaa aatgcattga ttttctgtat
     3361 gcacttcggc acaacacagc tcacaagtcg aaatcgtacc tttgcttcat gaacggcaag
     3421 tatgatctga gaggcgtttc gtcgggtaaa gaagaggtac atgaggctat aaaagacctg
     3481 tcaaagggac tatttccatt ggctttctgt aagatcctgc cagacgtagc cgggcaggat
     3541 cctgatcatt gcaacatcat gcatgccgat acagcaggta ctaaaactgc acttgcctat
     3601 ctgtactgga gggaaacagg aaacaccggg gtctgggaag gcatagccca ggatgcactg
     3661 gtcatgaacc tcgatgatat ggcctgtgtt ggcgtaaccg atcagtttct catttcctcc
     3721 accattggcc gaaacaaaca cctgataccg ggagaagtca tcgcagctgt gatcaaaggc
     3781 accaaccgct ttatcgaaaa catggctgaa cagggcatac atattcacca cagtggaggt
     3841 gaaactgcag atgtaggtga tattgtgcgc actatagatg taggtatcac tgcctttgcc
     3901 agaataccaa aatcatcttt gctggtgaac cggatcaaac cgggcaatgt gatcgttgga
     3961 ctggcctctt tcggtcaggc ttcttatgaa tctgcttata atggtggaat gggcagcaac
     4021 ggactaacct cagcacgtca tgatgtactc caccatgatt attcctccgt gtatccggaa
     4081 tccttcagcc ctgaaactga tgaagctgtg gtctattccg gcagcagaaa gcttacagac
     4141 ccatcgccgg tagaaggaat tgacaccggc acactgatcc tctctcccac ccgaacctac
     4201 ctgcctgtca tcagagagat acttgtgaac caccgcgagg ccattgacgg catgatccat
     4261 tgcacaggtg gcggccagac aaaggtgctg cactttgcag aaaatgtaca catcatcaaa
     4321 gacaacctct ttcctgtacc tcccttgttc cggatgattc aggaagaaag ccgtaccgac
     4381 tggaaagaga tgtaccgggt ttttaacatg gggcatcgcc tggagatcta ttgcagcgaa
     4441 gaggctgcag aaggtatcat gaagatcgcc tcatcctatg ccattgacag caggatcatt
     4501 ggccatgtaa cctccaacca gggagcccgt gtaaccgttg ctggtgaaga caatacttat
     4561 atatattcct aatattttac taccattcag gcttagccta cttttacaaa aattttctgt
     4621 atgcaaacca tttcatcagc ccgctttata gaattggaag aacgctacgg cgctcataac
     4681 tatcacccgc tgccggtggt actcagtcgt ggcgaaggtg ctctggtctg ggatgtagaa
     4741 ggaaaacgat actttgattt cctctcagca tacagtgccg ttaatcaggg gcattgccac
     4801 ccgcgtctcg tagaagtgat gcgtgaacag gccggcatcc ttacccttac aagccgtgca
     4861 ttccacaata atctgctggg tgaatatgaa caatacataa cgaatatctt cggctacgat
     4921 aaggtgttac ccatgaatag tggtgtagag gctgtggaaa cggccatcaa gctatgcaga
     4981 aaatggggct atcaggtgaa gggaattgaa cctggcaaag cccgtatcat tgtactggaa
     5041 gataatttcc acggtcgcac aacaggggtt atcagcttct ctactgaccc ttcctctacc
     5101 actgattttg gcccgttcat gccaggctat gaaatggttc cttacaatga tcttcctgcc
     5161 ctggaggcag ctctgaagga tcctgatatt gccggcctgc ttattgaacc tattcaaggc
     5221 gaagcaggtg tggttgttcc tgacgatggc tatctgtctg ccgcatttga gctctgtcgt
     5281 aaacacaata ttctgttcat ggcagacgag atccagaccg gcctttgccg tacaggaaaa
     5341 atgctggcct gcgatcatga aaacatacac ccggatgtac tgatactggg caaagctttg
     5401 tcaggcggta tgatgccggt atctgctgta ctggcagatg atcctgtcat gctgtgtatt
     5461 cagcccggag aacacggctc tacttttggc ggcaacccgc ttgcatgccg cattgccatg
     5521 actgccctgg atattctgca ggaagaacag cttgcagaga atgccgaccg catgggtcag
     5581 atgctgcgca acgcactgca aaaaatgagc ggcaacgtga tcaggtctgt tcgcgggaaa
     5641 ggcctgttaa atgccatcgt ggtagaacct atgaatggca gagatgcctg ggatttctgc
     5701 ctgatgctga aagaaaacgg actgctggct aaacctactc atggcgacaa aattcgtttt
     5761 gcccctcctc tggtcatcac agaagaacag atgctggaat gcatagagat catcaggaag
     5821 accgaggcac aattcctggc ctgaccacct gcttgtactc cgacatctgc agcctgtgtc
     5881 cggccatggt agtctctctt tccatattag agcctatcag gatcagtctg ccggaggcat
     5941 gagacaataa caattgctga taccactcaa ctccttccag gtcaagatga gatgtgggtt
     6001 catccatgat cagcagactg gcgtcagaaa gaatggccag cgtgttgcgc aaacgctgtt
     6061 tcattcctga agaaaagaaa cgcaattcct tttttcggtg tgctgacagt ccggtagcct
     6121 ctgcaacagc atcagtgtcc agcccgcccc ggtagggttt cagtttgccc tgcatgcgaa
     6181 tgttctcctc gagggtgaat tcttccggaa ggtctatata aggagcagta actgccacat
     6241 gactgaaaat cttttcgcgg tcaatggcat gaccgtccag ccggtacctg atgctgcctt
     6301 cagatggtgt cagcatacct gcaaccattc ttaaaaaagt actctttcct gagccattgg
     6361 caccggttac agcccagatc tgtccgcttt caaaaacctg atctataccg cggtagatcc
     6421 agtcataatt aaatcgtttg gaaacacctg tcagttcaac cctcattcgc tgagatgtct
     6481 gaaacctttc atgacaccac cttttgatgt acggatgaaa gtaaggatct gatctctttc
     6541 ggcagatgca ggaaactctg cttcaatgat gcccagagcc ttggtggtat tgaagcctct
     6601 aacgaacaac gtccggtaga tatcctggat atgttcgatg gtctcagcgc tgaaaccgcg
     6661 tctgcgcaga ccgatggaat ttacccccgc atatgccagt ggttcacgtg ctgctttcac
     6721 gaacggaggc acatcctttc tgaccagcga cccacctgca atgaatgcat gcgccccgat
     6781 cttgatgaac tgctgcaccg ctaccatacc ttccaggatg acccagtcat ctatttcaat
     6841 gtgtcctgcc aggttcacac tgtttgccag tatcacgtga tcgccaatga tacagtcgtg
     6901 cgcaacatgg ctgtatgcca tcagcaaaca gttggagcca atagtagtag tgttggcata
     6961 actggtacct cggttaactg ttacgcattc gcggatggtg gtattatcgc caatgatcac
     7021 tgtagtattc tctccgcg
//
LOCUS       DAOMAX010000131         7022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1636_length_7022_cov_4.681786, whole genome shotgun sequence.
ACCESSION   DAOMAX010000131 DAOMAX010000000
VERSION     DAOMAX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1636_length_7022_cov_4.681786"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PK742_07855"
     CDS             complement(<1..513)
                     /locus_tag="PK742_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU76545.1"
                     /translation="MGGSFALRVAWRYIFSRKSTNAINIISGITMLGITIGSAALIIV
                     LSAFNGFGSLVIGLYGAFYPDITITPAQGKVFNANPDMMEDLGNIPGVLYISQTLEEN
                     AMLAYNEQEHIATIKGVDSIYSLVTAVDDSVFAGEYVLRFEHFGKEVDCAILGSGVAA
                     TLNVALGLGYP"
     gene            complement(514..858)
                     /gene="rbfA"
                     /locus_tag="PK742_07860"
     CDS             complement(514..858)
                     /gene="rbfA"
                     /locus_tag="PK742_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQU76546.1"
                     /translation="MESRRQKKIANLIREEMGTFLQREGANYYGSRFVTVTDATITPD
                     MAECKIFISVLNEKEPQQVVDLLNKHVGDIRKRFGQIMRRDLRIIPMLEFRLDDTLDN
                     VFRLEELFKQIK"
     gene            950..2770
                     /locus_tag="PK742_07865"
     CDS             950..2770
                     /locus_tag="PK742_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU76547.1"
                     /translation="MRGYFRILRYVLRFRRLAVLNLVFNILYALFNLFSILTLIPFLQ
                     VLFNSVPVATEKPDGGVGVTQLLHLINYHFGAYVAEHGQMQGLLVVCFSITIIFILKN
                     GCRYAALYYLAPLRNGVVRELRQNIFERMLQLPLSYFSEKRKGDIIARMTTDVNEVEW
                     SIMNTLESTFRDPLTILFFFIAMLLLSPQLTLFVLILLPVMGLVIGRIGKTLKRKSGK
                     AQDQLGLLLSIIEESLSGLRIIKAFNAERYQKAKFSRENRAHSQLLTSVLNRRELSSP
                     LSEVLAIFVVAALLWYGGRLVISGESVLTGEMFIGYIVIFANLINPAKSFANSYYFIQ
                     RGIASIERIEAILDAPIEIKENMDAISLKGFEQAIEYRNVSFNYQEDARVLHHINLRI
                     EKGRMIAIVGPSGSGKSTLADLLPRFHDATEGEILIDGHNLRNLRTTSVRNLLGMVSQ
                     DPILFNDTVFNNIAFGVDNPNMDDVIRAAKVANAHDFIMKLDKGYHTAIGDRGSKLSG
                     GERQRITIARAIYKNPPILILDEATSSLDTESEKLVQDALFKLMQNRTSIVIAHRLST
                     IQFADEIIVMQEGCIVERGNHSALIAKKGLYAKLVEMQAF"
     gene            complement(2824..5487)
                     /locus_tag="PK742_07870"
     CDS             complement(2824..5487)
                     /locus_tag="PK742_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQU76548.1"
                     /translation="MTLNKLILAGGVCLLFSQCDPTKKAVSSDDYTGTIDTSYTDSYD
                     YGEYDYGDYDYGDEYYYDESYVDDASADVSPWSQDVYRSSATRSHDLLHTALDVRFDW
                     ANAWLLGKASLTLKPYFYPSRTLVLDAKGMDIHQVAMVNKGAKTNLNWTYDSLQITID
                     LGREYTKDEQYTVYIDYTARPEDRPEGGSFAITSDKGLYFINRDGSVADKPQQIWTQG
                     ETEASSVWFPTIDKPNERTTDEIYITVDKKFATLSNGLLTDSHLNNDGTRTDHWVMDM
                     PHAPYLFMMAVGEFTIVKDKWKNKEVNYYVEPAYAPYARAIFGNTPEMMTFYSNVLGV
                     DYPWQKYSQVVVRDYVSGAMENTTATLHGDFLQQTDRELLDETWEDVISHELFHQWFG
                     DLVTTESWSNLPLNESFATYGEYLWREYKYGREDADEHLKGDYDSYMTEAESKQVDMI
                     RFNYEDKEDMFDSHSYAKGGCILHMLRKYVGDDAFFASLKLYLEDNKFQAVEIHNLRL
                     AFEEVTGEDLNWFFNQWFLNSGHPDLTIEKEFYEDLGLLEIRVTQNQIQPTPTYVLPV
                     DIDIYTGDQVERHRIIIDKRDQTFTFEMGAAPDAVIFDAENQLLAQISEEKPSEEWSF
                     QFHHSQMYLDRLKALYNLEFQQDYSEQATEVYTAALRDKFWGIRSYAVESISLENNWD
                     GPVADIILNMAQHDPKASVRASAIGRLESLGADKAIPVLKAALNDSSYAVIGNALSVL
                     SILDPSAALEGARRYQNEKNINVLAPVWGILAEEGNPIDNDYFLHMFDTYTSWEVYYL
                     MVYYQLYLQNQTDISIIQKGVDQFKEIALDEDGGWFKFFAGSFLNGIYEYYAENMDYQ
                     DDTTKQAWQAGMTYIEETLSAINGMWSDEEY"
     gene            5569..6057
                     /locus_tag="PK742_07875"
     CDS             5569..6057
                     /locus_tag="PK742_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQU76549.1"
                     /translation="MKKVRVNEQHLFEVDGDLVNGKAWAANLIATGDRFYHLLHDGRS
                     YTIEVLQQDAAGKQCTLRINGRTYEVGIQDPIDQLLDQMGLSSAAAGKMAQLQAPMPG
                     LILDIRVQAGDAVQKGDPLMVLEAMKMENVLKAEGEGIVKNILVAKGQNVEKGFVMME
                     FE"
     gene            complement(6059..>7022)
                     /locus_tag="PK742_07880"
     CDS             complement(6059..>7022)
                     /locus_tag="PK742_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQU76550.1"
                     /translation="FYRMSHDASARRVMQFEADQLHLAAARLGPSFHQATETILAHPG
                     NTIVCGIGKSGAIAQKLTATLCSTGTRAVFLHAAEAVHGDLGVYTPGDPVILISKSGA
                     TPEMVRLIPWFKEQGSAIIAIVGNMHSPIAEQARFVLDASVEKEADPLNLAPTASSTV
                     ALALGDALAVALMEGRGFTAEAFARNHPGGQLGRNLHLKVSDVMHPIDQVACLTPDAG
                     FRELIIAMSTYNLGAACILGDGRLIGLVTDGDIRRALLQHEQVDGLTVADLMTKDPIA
                     IEADASLKEAVDKMENRQSQIAVLPVVADGRCTGLVRIHDIYHQ"
BASE COUNT         1677 a         1737 c         1795 g         1813 t
ORIGIN      
        1 aggatagccc agccccaaag ccacattcag cgtagcagcc acacctgaac ccaaaatagc
       61 gcagtccact tccttaccga aatgctcaaa ccgaagtaca tactctccag caaagacaga
      121 atcatctaca gcggtaacca gtgaatagat ggaatcaacc cctttgatgg tagcaatatg
      181 ttcctgttca ttgtaagcca gcatggcatt ctcttccagc gtctggctga tgtacagtac
      241 gccgggaata ttgcccaggt cttccatcat gtccgggttg gcgttgaaga ccttgccctg
      301 cgcaggggta atggtgatgt caggatagaa agcaccgtac aatccgatta ccagactgcc
      361 gaaaccattg aaagcagaca gcacgatgat cagtgctgca gagccgatgg tgatacccag
      421 catggtgatg ccggatataa tattgatggc attggtcgat ttgcgggaga agatgtagcg
      481 ccaggctacc cgtaaagcaa aagacccgcc catttacttg atttgcttga acagctcttc
      541 gaggcggaat acattatcca gggtatcgtc gagccggaat tccagcattg gaatgatgcg
      601 caggtcgcgg cgcatgatct gcccgaaacg cttgcgaata tctcccacat gcttgttcag
      661 aaggtccacc acttgttgcg gttccttttc gttcagtacg ctgatgaaga tcttgcactc
      721 tgccatatca ggggtgatcg tggcatctgt tacggtcaca aagcgggagc cgtaataatt
      781 ggccccttcc cgctgcagga atgtgcccat ttcctccctg atgagattgg ctatcttctt
      841 ctgtctgcga ctttccatgg ctgcgtataa aggtacgaaa acagagggtg tgaccacaca
      901 gcaataaaca gcgctgctgc acgtcttgtt ttggtaactt tggctcgcta tgcgtggata
      961 cttcaggata ctcaggtacg tgctgcgttt tcgcaggctt gcggtactga acctggtatt
     1021 caatatcctg tacgcactgt tcaacctgtt ttccatcctg acactgatcc cctttctgca
     1081 ggtactgttc aattctgttc ctgtagcaac tgaaaagcca gatggtggtg ttggtgttac
     1141 ccagttgctt catctgatca attaccattt tggcgcctat gtggctgagc atgggcagat
     1201 gcagggattg ctggtggtct gtttcagcat cacgatcatt ttcattctta aaaatggttg
     1261 ccgttatgca gccttgtatt atctggctcc gctgcgcaac ggcgtggtca gagagttgcg
     1321 ccagaacatc tttgaacgta tgctgcaatt gccgctttct tatttcagcg aaaagcgcaa
     1381 aggcgatatc atcgcccgta tgaccacaga tgtgaatgaa gtggaatgga gcatcatgaa
     1441 taccctggag tccaccttcc gtgatcctct taccattctg ttctttttca ttgctatgct
     1501 gctgctgagc ccgcaactaa ccctgttcgt gctgatcctg ttgccggtca tggggttggt
     1561 tattggtcgc atcggcaaga ccctgaaacg caaatcaggc aaagctcagg atcagctcgg
     1621 tttgttattg tccatcatag aggaatcttt gtcagggttg cgcatcatta aagccttcaa
     1681 tgcagagcgc taccagaagg ccaagttcag tcgggagaac cgggcacact cacaactgct
     1741 tacttctgtt ctgaaccgcc gggaattatc cagtccgctg tctgaagtgc tggccatttt
     1801 tgttgtggca gctttgttat ggtatggcgg gcggctggtc atcagcggag aatctgtgct
     1861 taccggcgaa atgttcattg gctacatcgt catcttcgcc aacctgatca atcctgccaa
     1921 gtcattcgcc aacagctatt attttattca gcggggtatc gcttccatcg aacggataga
     1981 agctattctg gatgcaccga tagagatcaa agagaacatg gatgccatca gtctgaaagg
     2041 atttgaacag gccattgaat accggaatgt cagcttcaat taccaggaag atgcaagggt
     2101 gttgcaccac atcaatctgc gcatagaaaa gggacgtatg atcgccattg ttggtccgtc
     2161 cggttcgggt aagtccaccc tggctgatct ccttccgcga ttccatgatg ctacagaagg
     2221 agagatcctc atagacgggc ataaccttcg taacctgcgc accacttctg tgcgcaacct
     2281 gctcggcatg gtcagtcagg accctatcct gttcaatgat acggtgttca acaacatcgc
     2341 ttttggtgtt gacaatccca atatggatga tgtgatacgg gcagccaaag tggccaatgc
     2401 acacgacttc atcatgaaac tggataaggg ctaccatact gcaataggcg acaggggctc
     2461 taaactcagc ggtggcgaac gccagcgtat caccattgcc cgtgccatct ataagaatcc
     2521 tcccatcctc attctggatg aagctacttc ttctctcgat acggaatcag aaaaactcgt
     2581 gcaggatgcg ctgttcaagc tgatgcagaa cagaacatcc attgtcattg cccaccgcct
     2641 gagcaccatc cagtttgctg acgagatcat tgtgatgcag gaaggttgca tcgtagaaag
     2701 aggcaaccac agtgccctga tagccaagaa aggtctgtat gccaagctgg tggagatgca
     2761 ggcgttctga ccgggcaaga aaaaagccct ccggagaggg ctttcaatac aacaaaaata
     2821 atatcagtat tcttcatctg accacatgcc attgatggct gacagggtct cttcgatata
     2881 ggtcatgcct gcctgccagg cttgtttggt ggtgtcgtcc tgatagtcca tattctctgc
     2941 atagtattca tagataccat tcaggaagct tccggcaaag aacttgaacc agcctccgtc
     3001 ttcgtcaaga gcgatctctt tgaactgatc tacccctttc tgaatgatgc tgatgtcagt
     3061 ctggttttgc agatacaact gatagtaaac catcaggtag tacacttccc agctggtata
     3121 ggtatcaaac atgtgcagga agtaatcatt gtctattgga ttaccttctt ctgccaggat
     3181 accccaaacc ggtgccagca cattgatgtt cttttcgttc tgatagcgcc ttgctccttc
     3241 cagggcggcg gaaggatcca gtatgctcag aacactcagg gcattgccga tgaccgcata
     3301 ggaagaatcg ttcagtgcgg ctttcagcac cggaatggcc ttgtctgcac ccagtgattc
     3361 cagccggcca atggcagatg cacgaacaga cgccttaggg tcgtgctgcg ccatattcaa
     3421 aatgatgtct gccacaggtc catcccagtt gttttcaaga gaaatgcttt caactgcata
     3481 agaacgtatg ccccagaact tatcccgcag tgcggcagta tatacttcag tagcctgttc
     3541 actgtagtcc tgctggaatt ccagattgta caatgccttc aaacgatcca gatacatctg
     3601 actgtggtga aactggaatg accattcttc tgatggcttt tcttcagaga tctgcgccag
     3661 taactgattc tctgcatcaa aaatgaccgc atcaggcgca gcacccattt caaaggtgaa
     3721 ggtctggtcg cgtttgtcta taatgatgcg atggcgctcc acctgatcgc cggtgtagat
     3781 gtctatgtca accggcagta cataggtagg tgtaggttgt atctgattct gggtaaccct
     3841 gatctccagc agaccgaggt cttcatagaa ttctttttct atggtcaggt cggggtgacc
     3901 actgttgaga aaccactgat tgaagaacca gttgaggtct tcgccggtca cttcttcgaa
     3961 agcaagacgc aggttatgga tctcaactgc ctggaattta ttgtcttcga ggtacagctt
     4021 cagcgaggca aagaaggcat catcacccac atacttgcgc agcatatgaa ggatgcatcc
     4081 gcctttggca taactgtggc tgtcaaacat gtcttccttg tcttcataat tgaagcggat
     4141 catatcaacc tgcttggatt cagcctcggt catgtaagaa tcgtagtcgc ctttaaggtg
     4201 ttcatcggcg tcttcccgtc cgtatttgta ttcccgccag aggtattcac cgtaggtagc
     4261 gaatgattcg ttgagtggca ggttgctcca tgattcggtg gtcaccagat cgccgaacca
     4321 ttggtggaac agctcgtggc tgatcacatc ttcccaggtc tcatccagca gttcacgatc
     4381 ggtctgctga agaaaatctc cgtgcagcgt agcggtggtg ttttccatgg caccggacac
     4441 ataatcacgt accactacct gtgaatattt ctgccacggg taatcaactc cgagtacatt
     4501 gctgtagaat gtcatcattt caggggtgtt gccgaagatg gcacgggcat atggtgcata
     4561 agccggttct acatagtagt tgacctcctt gttcttccat ttgtctttga cgatggtgaa
     4621 ttcgcccacc gccatcatga acaggtaagg tgcatgcggc atgtccatga cccagtggtc
     4681 tgttcgggtg ccgtcgttgt tcagatgaga atctgtaagc agtccattgg acagggtggc
     4741 gaatttttta tctactgtga tatagatctc atcggtggtg cgctcgttcg gtttgtctat
     4801 ggtagggaac cagacggaag aagcttcggt ctcgccctgg gtccagatct gttgtggttt
     4861 gtctgctacg ctgccatctc tgttgatgaa atacagaccc ttatctgagg tgatggcaaa
     4921 gctgccacct tccggccggt cttccggtct ggcagtatag tctatataga ccgtgtactg
     4981 ttcgtctttc gtgtattcac gaccgaggtc aatggtgatc tgcagactgt cgtaggtcca
     5041 gttgaggtta gttttggctc ccttgttcac catggccacc tggtgtatgt ccatgccttt
     5101 tgcatcgagc accagggtgc ggcttggata gaaataaggc ttgagtgtga gcgaggcctt
     5161 gcccagcagc caggcgttgg cccagtcaaa gcgcacatcc agtgccgtat gcagaagatc
     5221 gtgacttctg gtggctgaag acctgtacac atcctgactc catgggctta catcagcgga
     5281 agcatcatct acgtaagatt catcgtaata gtattcatct ccgtagtcgt aatcgccata
     5341 gtcatattct ccgtaatcat aggagtctgt atagctggta tcaatcgtgc ccgtgtagtc
     5401 atcagagctg acagcttttt tggtcgggtc acattgcgaa aagagcagac atacaccgcc
     5461 tgcaaggatc agtttgttca aagtcatgtg aaagatttac ttttaccgag gatgcacaaa
     5521 attaggattt taacacatct gaagtcccca tttaacagag tttaaggtat gaagaaggta
     5581 agggtcaatg aacagcattt atttgaggtg gatggtgacc tggtgaacgg aaaggcctgg
     5641 gccgccaacc tgatcgccac cggcgatcgt ttttatcatc tgctgcacga tggccggtcc
     5701 tataccatcg aagtactgca gcaggatgca gcaggaaagc agtgcaccct tcgaatcaac
     5761 ggcaggacct atgaagtagg aatccaggat ccgatagacc agttgctgga tcagatgggg
     5821 ctttcttcag cggcagcagg caaaatggcc cagctccaag cacccatgcc gggtctgata
     5881 ctggacatcc gggtgcaggc gggcgatgcg gttcagaaag gtgatccgct catggtgctc
     5941 gaggcaatga aaatggagaa cgtactgaaa gccgaaggtg aaggaatagt caaaaatatc
     6001 ctggtagcga aaggccagaa tgtagagaag ggcttcgtga tgatggaatt tgaatagact
     6061 actggtgata gatatcgtgg atgcgtacca gaccggtgca gcgtccgtca gccacgaccg
     6121 gcaatactgc gatctgcgac tgccggtttt ccattttatc tacggcttct ttcaggctgg
     6181 catctgcttc gatggcaatg ggatctttgg tcatcagatc tgcaacggtc aatccgtcca
     6241 cctgctcatg ttgtaacagg gcacgtcgaa tatcaccgtc ggtcaccagg ccgatcaatc
     6301 ttccgtcccc gagaatacag gccgcaccca gattgtatgt actcattgca atgatgagtt
     6361 cgcggaatcc tgcatcgggt gtcaggcatg ccacctgatc aatggggtgc atgacatcag
     6421 atactttcag gtgcaggttg cgacccagct gtccgccggg atgattgcgg gcgaaggctt
     6481 ctgcagtaaa accgcgtcct tccatgaggg caacggccag cgcatctccc aatgccagtg
     6541 ccaccgtgct gcttgcggtg ggtgccagat tgagcggatc ggcctctttc tctaccgacg
     6601 catccaggac gaaacgggcc tgttctgcaa taggagaatg catgtttcct acgatggcga
     6661 tgatggcaga accctgctct ttgaaccatg gaatgagcct gaccatttca ggcgtggcac
     6721 cgcttttcga aataagtatg accggatcgc cgggggtata gaccccgagg tcaccgtgta
     6781 ctgcttcggc ggcatgcagg aaaactgccc tggtgccggt gctgcacaag gtagctgtca
     6841 gcttctgggc gatggcacct gatttaccga taccacaaac gatggtattg cccggatgtg
     6901 ccaggatggt ttctgttgcc tgatgaaaag agggccccag acgtgctgct gccagatgta
     6961 attgatcagc ttcaaattgc atgaccctgc gggccgaagc atcgtgcgac atgcggtaaa
     7021 aa
//
LOCUS       DAOMAX010000132         6927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1673_length_6927_cov_3.798749, whole genome shotgun sequence.
ACCESSION   DAOMAX010000132 DAOMAX010000000
VERSION     DAOMAX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_6927_cov_3.798749"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PK742_07885"
     CDS             complement(<1..1640)
                     /locus_tag="PK742_07885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQU76551.1"
                     /translation="MKRFLVVFVLFTPICMRAATPTISDSLDILHTTVHLEITDYAGK
                     TINGYAELEVQAKVDGMDLLPLDLMTLTVDSVWMDGVSTAFTHAGELLSIPLTPAMNT
                     GDTRTFRIFYGGEPDQDASWGGWYWSGDYSYQMGVGFDADPHNFGRIWFPCFDNFIER
                     SSFTFEIITPSGYKAFCGGTLESESDNGDGTTTWIWQLPQPIPSYLASVAVSTYSTVA
                     WQHTGIASDYPVQLGVKPSDSTKMKNSFINLDAAIEAFENAYGPYRWDRVGFTVVPFF
                     GGAMEHATNIAYPSFAVDNTLTWETLYAHELSHMWWGDLITCSDQSEMWLNEGWASFS
                     EYLFTEALYGRKAYEDMVKEQHLEIIHYGFARDGGNYFPLAEMPHEYTYGFTTYTRGA
                     MTVHNLRGYMGDELFFSCITEFLDEYAFQPVTSEQFRDHLSECSGIDMTPFFDGWIFQ
                     AGTPAVEVDYFSYTADDLGICIEQKLNHAPDYFTNYPIEVSLVDKQGHTLYAETVHAN
                     GPNDEVHINAPALDSFYVVLDRNNLIADAVTADEIFLTT"
     gene            1659..2570
                     /locus_tag="PK742_07890"
     CDS             1659..2570
                     /locus_tag="PK742_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76552.1"
                     /translation="MFRATKLSTFILAMGWLLPMEAQTDTLFLTPVIHLDSVVISEVA
                     DGFSVGEFIHLMEEDTGFYQAFRNLRTITYSSDALVRMFDDEGLTEASYSNSAKQILE
                     NHCRHLEFQQEHTTGNFFDKHGEAEYYTARLFSHIFLYRDTVCSSQSTAAVHDPSGLE
                     KRKEQLKVLMFQPGQPVEGIPFIKNELGVFEEDMTALYDYDMEAVEFRARPCFKFTIE
                     KKAEVSEKKVVLQSMETWFDRSTYQIVARNYHLKEDNFTYDFDVHMQVLLGQREGKEV
                     PVWIYYNGSWDVPGKKRETGSVQITVR"
     gene            2666..2890
                     /locus_tag="PK742_07895"
     CDS             2666..2890
                     /locus_tag="PK742_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76553.1"
                     /translation="MKIRNLLLGFTLFALTTAFSDQYGRDVYICKGPQSTKYHYISDC
                     RGLSNCSSDIYRVSLDEAKSMGRTLCGWED"
     gene            complement(2947..3774)
                     /gene="panB"
                     /locus_tag="PK742_07900"
     CDS             complement(2947..3774)
                     /gene="panB"
                     /locus_tag="PK742_07900"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQU76554.1"
                     /translation="MSTTRQDFSKVKRITTHTIQEMKQNGVKISMLTAYDFSMARILD
                     EAGIDVLLVGDSASNVMAGHETTLPITLDQMIYHATSVVRGVQRALVVVDLPFGSYQG
                     NSKTALESTIRIMKESGAHAVKLEGGREVKESIERILSAGIPVMGHLGLTPQSIYKFG
                     TYTVRATEEAEAQRLQEDAILLEQLGCFSIVLEKIPADLGGKVAKSVHIPIIGIGAGP
                     EVDGQVLVMHDMLGLTKEFKPRFLRRYLNLFDDIRNAVHQYTEDVKSGDFPNDQEQY"
     gene            3992..6298
                     /locus_tag="PK742_07905"
     CDS             3992..6298
                     /locus_tag="PK742_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQU76555.1"
                     /translation="MMNAFSRRLLRLGIPLVVLLIAAFFLYRIRHRVLPSNDITLEFT
                     EANAGIDGMAAWLAAYADLVLVPDNSYAPDAALKKYLSIPEPEKPMEKMQWNANVARS
                     YLNTGKPEESFPYFKEYFRIAEENPAAIRSNKFNKEYYRFILETYGISYMRQGEIANC
                     LNRHTPESCIFPIQGGGIHADTMGAANAFRIYRSILAEDPGDHEAVWLLNVSAMALGV
                     YPDSVPEKFLLHPNRFASAYDIGRFTDVAPGAGVAFNSLSGGVCMDDFDNDGYLDIIC
                     SGWQLTERIAYFHNNGDGSFDDWSASSGLGKLAGGLNMVHADYDNDGLEDVLVLRGGW
                     WDKYGCMPNSLLRNLGNGKFEDMTWQAGLHGMHPSQTARFEDFNKDGWLDIFVGNEAT
                     PGSPDHPCELYISNGDGTFKEVGKKTGAGVKAFVKASGTADYDNDGDVDIILSVYKGK
                     NILLQNRLSETGKLQFKDVSDEAGISGPNMSFPCFFFDFNNDGYEDIYISSFHQQSME
                     NVAAWYRDWPYEHEYGALYRNNGDGTFSNITTDANLEIPSLGMSCNFGDIDNDGYLDF
                     YLGTGNPDYRSLVPNLMFRNDGNGGFQNITTSGGFGHLQKGHGIAMGDVNRDGDLDIY
                     AVMGGALTGDYFWNALYDNPGQGNHFIGLRLVGKKSSRDPIGARVVVHCTDPNGYQRE
                     ICRTLNSGGSFGSSPMEMTIGLGIYDLIEKIEITWPDGSLQTLVNPDPDARYKVVQGN
                     DQIQKLTYRTFTFATDGGHGAHHMNMDH"
     gene            6300..>6927
                     /locus_tag="PK742_07910"
     CDS             6300..>6927
                     /locus_tag="PK742_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76556.1"
                     /translation="MRLTPLLIGSVLLVMTSCGKSSRKPDYAQNEVAWPDNMPEHQAM
                     VKYLADLQQQYFLQPGNRYASTMRAKKMLAQEPPKDEKKRMQFIYETAFECLSAGMTD
                     QAIQLLEENLEQAAKMPPAERSRMRNLLGICYLRYGEQQNCISNHVAESCILPIDSKA
                     MYTVREATEKGYALYQEILKDEPDNLNAIFLLNVAAMNLGYYPDKVPSQ"
BASE COUNT         1816 a         1652 c         1734 g         1725 t
ORIGIN      
        1 gttgtcagga atatttcatc ggcagtgaca gcatcagcta tcaggttgtt ccggtccaga
       61 accacataaa atgaatccag tgccggtgca ttgatatgaa cttcatcgtt gggcccgttt
      121 gcgtgaactg tttctgcata gagggtatgt ccctgtttgt ccaccagaga tacctctatc
      181 ggataattgg taaaataatc cggtgcatga ttgagttttt gttctatgca tatgcccagg
      241 tcatcggcag tgtaggagaa gtagtccact tccaccgcag gtgtaccggc ctgaaatatc
      301 cagccatcaa agaagggcgt catatcaatt ccgctgcact cgctcaggtg atcacggaat
      361 tgttcgctgg taaccggttg aaaggcatat tcgtccagga attctgtgat acaggagaag
      421 aacagctcat cacccatata tcctctcagg ttgtgtacgg tcatggcacc acgggtatag
      481 gtagtgaatc cataggtata ttcatggggc atctctgcca gtggaaagta gttgccgcca
      541 tctctggcaa agccataatg tatgatctcc aggtgctgct ctttcaccat atcttcatag
      601 gctttcctgc cgtatagtgc ttcggtgaac agatactcag aaaagctggc ccatccctcg
      661 ttcagccaca tttcactttg atctgaacaa gtgatcagat cgccccacca catgtgagaa
      721 agctcatgtg catagagggt ctcccaggtg agcgtattgt caactgcaaa actgggataa
      781 gctatattgg tggcgtgctc catggctccg ccaaagaaag gaactacggt aaaaccaact
      841 cggtcccaac ggtaagggcc ataggcattt tcaaaagctt caatggccgc atccaggttg
      901 atgaaactgt tcttcatttt ggtactgtcg gacggcttca cacccagctg cacgggatag
      961 tctgaagcaa ttccggtatg ctgccaggca acggttgaat acgtgcttac tgccaccgat
     1021 gccaggtaac tcggtatagg ttgcggaagt tgccagatcc aggtggtggt accatcgccg
     1081 ttatctgatt cagattccag cgttccgcca caaaaggctt tatacccgga aggtgtgatg
     1141 atctcgaaag taaaggaaga acgttctatg aaattgtcaa agcagggaaa ccagatcctt
     1201 ccgaagttgt gcgggtcagc atcaaaacct acacccatct ggtaagaata gtcgccgctc
     1261 cagtaccagc caccccagct ggcatcctga tcaggttcac cgccatagaa aattctgaag
     1321 gtccgggtat cgccggtgtt cattgccggt gtgagcggaa tactcaggag ttcacccgca
     1381 tgtgtgaaag ctgtgctgac tccgtccatc cagacagaat caacggtaag cgtcatcagg
     1441 tcaagaggaa gcagatccat gccatctact tttgcctgta cttccagttc tgcatagccg
     1501 ttgatggtct tgccggcata atctgtgatc tccagatgca cggtggtgtg cagaatgtcc
     1561 agcgaatcgc tgatggtagg tgtggcagcc cgcatacaaa tgggagtaaa caggacgaaa
     1621 acaacgagga atctcttcat gatgaatacc tttgaatcgt gttcagagcc actaaattaa
     1681 gcactttcat actggccatg ggttggttgt tgccgatgga agcccagaca gatacccttt
     1741 tcctgacgcc ggtgatacac ctggacagcg tggtgatcag tgaagtggcc gatggttttt
     1801 cagtgggcga attcattcat ctgatggaag aagataccgg tttctaccag gcattccgaa
     1861 acctgcgcac catcacctac agcagcgatg ctctggtgcg tatgttcgat gatgaagggc
     1921 tgactgaggc atcctattcc aacagtgcga agcagatcct ggagaatcat tgccggcatc
     1981 tggagttcca gcaggagcat accaccggta attttttcga caaacatggt gaggcagagt
     2041 actacacggc caggttgttt tcgcacatct ttttgtatcg cgatacggtt tgcagttcac
     2101 agagcactgc agctgttcat gatccttccg gactggagaa aagaaaggaa caattaaaag
     2161 tgctgatgtt tcagcccggg caaccggtgg aagggatccc tttcattaaa aatgaactgg
     2221 gcgtttttga agaggatatg accgcactgt atgactatga catggaagct gtagagtttc
     2281 gggccaggcc atgctttaaa ttcaccatag agaagaaggc ggaggtatct gaaaagaaag
     2341 tggtgttgca aagtatggag acctggtttg accggtccac ctaccagatc gtggcacgca
     2401 attatcacct gaaagaagac aatttcacct acgattttga tgtgcacatg caggtgctgt
     2461 taggtcagcg ggaagggaag gaggtgccgg tctggatcta ctacaacggc agctgggatg
     2521 tgccgggcaa aaagagagaa acaggcagtg tgcagatcac ggtgaggtga tgatgcattt
     2581 tgatgatttt ccgcatttgc atccgtcttt cggatgtatc gctatgaaac aatcctgatg
     2641 tgtatcttta agaaaatgca cacccatgaa aatcagaaat ctactgcttg gattcacgtt
     2701 gttcgcacta accactgcat tcagtgatca gtatggccga gatgtatata tctgtaaagg
     2761 gcctcagtca acgaagtatc attacatcag cgattgcagg ggcttaagca attgttcatc
     2821 agatatttac agagtttcgc tcgatgaagc aaagagcatg ggaagaacgc tttgtggctg
     2881 ggaagactag tgtctgttaa gttttacaac caacttatca agatccgttg aatatgagca
     2941 gtattatcag tactgttcct gatcgttcgg gaaatcgcca gacttcacat cttccgtgta
     3001 ctgatgcacc gcgtttcgga tatcgtcgaa caagttcaga taccgtcgca ggaatctggg
     3061 cttgaattct ttggtcagtc ccagcatatc gtgcatcacc aaaacctgac cgtccacctc
     3121 cggccccgca ccgataccaa tgatgggaat atgtacgctc ttcgccacct ttccgcccag
     3181 atctgctggt atcttttcca gtacaatgga aaagcatccc agttgttcca gcagaatggc
     3241 atcttcctgc aggcgctgag cttctgcttc ttccgttgcc ctgacagtat aggtgccaaa
     3301 tttatagatg gactgaggtg tcagccccag gtgccccatg accgggattc ctgcagacaa
     3361 tatgcgttct atgctttctt tcacctcccg tccgccttcc agtttcaccg catgtgcacc
     3421 ggattctttc atgatgcgaa tggtgctttc cagagcagtc ttgctgttgc cctggtaaga
     3481 accgaaaggc aggtccacca ccaccagtgc ccgctgcaca ccacgaacca ctgaggtggc
     3541 atgatagatc atctgatcaa gagtgatggg taaggtggtc tcatgtccgg ccatgacgtt
     3601 ggaagctgag tcgccaacaa gcaacacatc aataccggcc tcatccagga tgcgggccat
     3661 agaaaaatca taggcggtca gcatactgat cttcaccccg ttttgtttca tctcctggat
     3721 ggtatgggtg gttatccgtt ttacttttga aaagtcctgt ctggttgttg acataaatta
     3781 aaattgcccg aaagttaagg aatgtgcatg agtaccttgc ataaatacca gtaagccgaa
     3841 gcaggccact gcacacatga tccaccgtat ggtaatagca gcaatgaagc tcaggatatc
     3901 agtcacagcc gaaccatcca tgagattctt tgttttcttt ttaaataaat tataaatgtg
     3961 caaccactac tgtaaatatt acctctaact aatgatgaat gctttttcca gacgactcct
     4021 ccggctggga atacccttgg ttgttttgtt gatcgcggct ttttttctgt accgcatccg
     4081 tcatcgggta ttaccttcca atgatataac cttagaattt acagaggcaa atgccggaat
     4141 tgacggcatg gccgcctggc tggcagctta tgctgacctg gtgctggttc ctgacaattc
     4201 gtacgccccg gatgcagcac tgaaaaaata cctcagtatt ccggaaccgg aaaaacccat
     4261 ggaaaagatg cagtggaatg ccaacgttgc caggtcatac ctgaataccg gtaaaccgga
     4321 agaatccttt ccatacttta aggagtattt ccggatcgcg gaagaaaatc ctgcagcaat
     4381 acgatccaac aagtttaaca aagagtatta caggttcatc ctggaaacct acggtatcag
     4441 ctatatgcgg cagggagaaa ttgccaattg cctgaatcgc catacccccg agtcctgtat
     4501 tttcccgatt cagggtggag gtattcatgc ggacactatg ggcgctgcca atgccttcag
     4561 aatataccgg tctatccttg cagaagatcc tggtgatcat gaggcagtct ggttgctcaa
     4621 cgtttctgct atggccctgg gcgtgtatcc ggactcagta ccggaaaaat ttcttctgca
     4681 tccaaacaga tttgccagtg cctatgacat agggcgcttt accgatgtag ctccgggagc
     4741 aggtgtggct ttcaattcgc tgagcggtgg cgtgtgtatg gatgattttg acaatgatgg
     4801 ctatctggac atcatctgca gcggctggca actaacagaa cgcatcgcct acttccataa
     4861 taatggtgat ggcagtttcg atgactggtc tgcttcttcc ggtctgggaa agctggccgg
     4921 aggacttaat atggtccatg cagattacga caatgatgga ctggaagatg tacttgttct
     4981 gcgtggaggt tggtgggata aatacggttg tatgcctaac tccctgctgc gcaatctggg
     5041 caacggtaag tttgaagata tgacctggca agcgggtctg cacggcatgc atccttctca
     5101 aacggcacgc ttcgaagact ttaataaaga cggatggctg gacatttttg tggggaatga
     5161 agccacaccc ggaagcccgg accatccctg cgagttgtat atcagcaacg gcgatggcac
     5221 attcaaggaa gttggaaaaa aaacaggtgc cggcgtgaag gcttttgtaa aggcatccgg
     5281 tacggccgat tacgataacg acggagatgt ggacatcatc ttgtcggtat ataaagggaa
     5341 aaatattctc ctgcagaaca gactctctga aacgggaaag ctccagttca aagatgtttc
     5401 agatgaagcc ggtatttccg gtccgaacat gagtttcccc tgttttttct ttgatttcaa
     5461 caacgacggc tacgaagaca tctacatatc ttccttccat cagcaaagca tggagaatgt
     5521 ggcagcatgg taccgcgact ggccctatga acatgaatac ggagctctct accgaaacaa
     5581 tggagatggc accttctcga atataaccac agatgccaat ctcgaaatac cctctctggg
     5641 tatgagctgt aatttcggcg atattgataa cgacggctac ctcgactttt atctgggtac
     5701 cgggaatcct gattaccgca gtctggtgcc aaacctgatg ttccgcaatg atggcaatgg
     5761 aggcttccag aacatcacca cctcaggtgg attcgggcat ttgcaaaaag gacatggtat
     5821 tgccatgggc gatgtgaacc gggatggcga cctggatatc tatgcggtca tgggtggagc
     5881 actgaccggc gattatttct ggaatgccct gtatgataat ccaggccagg gtaatcattt
     5941 catcggacta agactggtag gtaaaaagtc cagccgtgat cccattggtg ccagggtagt
     6001 ggtgcactgc acggacccga acggctacca gcgggagatc tgtcgtaccc ttaattccgg
     6061 cggcagcttt ggttcatcgc caatggaaat gaccatcggc ctgggtatct atgacctgat
     6121 tgagaaaata gagatcacct ggccggacgg tagtctgcaa actcttgtaa atccggatcc
     6181 cgacgcccgg tataaggtgg tccagggaaa tgatcagatc cagaaactga cctacaggac
     6241 atttaccttt gcaactgatg gcgggcacgg agcccatcat atgaacatgg atcactgaca
     6301 tgcgtcttac acccttactg ataggaagtg ttttactggt tatgacttcc tgcggaaaaa
     6361 gctcccgcaa acctgattat gcacaaaatg aagtagcctg gccagacaat atgccggaac
     6421 accaggccat ggtgaagtac ctggctgacc ttcagcagca atatttcctg caacccggta
     6481 accggtatgc cagcaccatg cgggcaaaga aaatgctggc gcaggaaccg ccaaaagacg
     6541 agaaaaaaag aatgcagttc atctatgaga ccgcattcga atgcctctct gccggtatga
     6601 ccgaccaggc cattcagctg ctggaagaaa acctggaaca ggcagcgaaa atgccgccag
     6661 cagaacgcag cagaatgcgc aacctgcttg gaatttgtta tttgcgttat ggcgaacagc
     6721 agaactgtat ctccaaccat gtagccgagt cctgcatcct gcccattgac agcaaggcca
     6781 tgtacacggt tcgtgaagct accgaaaaag gatacgcctt ataccaggag atcctgaagg
     6841 acgaaccgga taatctcaat gccattttcc tgttgaatgt ggcggccatg aacctcggtt
     6901 attatccgga caaggtaccc tcacagt
//
LOCUS       DAOMAX010000133         6889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1685_length_6889_cov_3.822212, whole genome shotgun sequence.
ACCESSION   DAOMAX010000133 DAOMAX010000000
VERSION     DAOMAX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_6889_cov_3.822212"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK742_07915"
     CDS             complement(<1..381)
                     /locus_tag="PK742_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76557.1"
                     /translation="MHSRLLLSLLLLSPVALIAQTNGQGLFGVDLVIGLPQDEFKEVN
                     KDALVGVMVNFLYQPSPDIPISIGGDLGWMGNGRKVQYEELTADITAGGVLIDQLYFP
                     LRIETSNNVFTGHLLVRATAPTKIF"
     gene            complement(426..1154)
                     /locus_tag="PK742_07920"
     CDS             complement(426..1154)
                     /locus_tag="PK742_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQU76558.1"
                     /translation="MTSSLIIIPTYKEVENIGLMIPAVLDQDPSYALLIVDDGSPDGT
                     ADAVRGLMKQYPDRLHLLERPGKQGLGTAYILGFRWALERGYSFVLEMDADFSHDPKD
                     LPRLIKACSEGADVSVGSRYVKGGRVENWPADRVLISKGASIYVRLITWMPVRDTTAG
                     FVCYKREVLEAIDLDKIRFIGYAFQIEMKYAAWRSGFRIREIPITFKDRVKGSSKMSK
                     GIVKEAALGVLSIRWKSLFHAYRK"
     gene            complement(1157..1924)
                     /locus_tag="PK742_07925"
     CDS             complement(1157..1924)
                     /locus_tag="PK742_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQU76559.1"
                     /translation="MLHLDKPLVFLDLETTGVNIATDRIIEISMLKLYPDQQQEIRTD
                     RVNPGIPIPAESTMIHGISDADVKDKPSFRKIAPEIRDFIGNADMAGYNSNKFDIPLL
                     IEEFLRADVGFDTNRKYVDVMRIFTLMEKRDLQTAYRFFCNKELVNAHSAEADVIATF
                     EVLNGQLERYGDQLENNVSFLHEFTKDGDFVDTGRRMVFKDGKEYFNFGKYKDRMVEE
                     IYKKEPHYFDWILQNEFPLDTKLKLKMIRMRLQQKNK"
     gene            complement(1937..3283)
                     /locus_tag="PK742_07930"
     CDS             complement(1937..3283)
                     /locus_tag="PK742_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQU76560.1"
                     /translation="MEEKGKIHLIAIGGAVMHNLALALQEAGYQVTGSDDEIFEPSLN
                     RLAKAGLLPEHMGWFPERITADLDRVILGMHARSDNPELLEATRIGLPVMSFPEFIYS
                     VSAEAVRMAICGSHGKTTITSMLMHGLRAAASHFDYLVGAQLEGFQTMVRLSQAPLMI
                     IEGDEYHASPLLPEPKFLFYKAQILLISGIAWDHFNVFPTFDDYLMTFRKLLRQMQPA
                     DKLVANAEDEVLVRLVQEEVAPRQVIWYTPFRYHTDVSGTTIEYDGTSYPISVFGKHN
                     ISNLSGAATLAGLAGIDTSSFLGHLSDFTGAARRMEKAYERKGKTVFRDFAHAPSKVK
                     ATTSAIREMYPTQRITACLELHTFSSLNKGFLPQYAHALDAADIRVVFFDPHTLEMKK
                     LPPVSKEEVQDFFQDPGLLVCNDLQEMTRHLQASSFDILLLMSSGHFAGLNIDGLV"
     gene            3383..3529
                     /locus_tag="PK742_07935"
     CDS             3383..3529
                     /locus_tag="PK742_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76561.1"
                     /translation="MFTVAKKSRWIIVALILSMAVVGLSSCHTEGCPNKITQVNPFGA
                     DDVS"
     gene            3533..3892
                     /gene="ytxJ"
                     /locus_tag="PK742_07940"
     CDS             3533..3892
                     /gene="ytxJ"
                     /locus_tag="PK742_07940"
                     /inference="COORDINATES: protein motif:HMM:NF022462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HQU76562.1"
                     /translation="MINWIDLTDEAVWQDVVRRSHIHPVCIFKHSTRCGVSSMVLNRL
                     EQHEPVQNSSWYMLDMIRYRTFSDLIAESTGVVHESPQMIILSDGAVQIHFSHTEIRT
                     EVISQWLQQLQRKDPVL"
     gene            complement(3846..4271)
                     /locus_tag="PK742_07945"
     CDS             complement(3846..4271)
                     /locus_tag="PK742_07945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQU76563.1"
                     /translation="MIVSKVTYTGKLHTEAVHLASGSRIQTDAPVDNHGKGEAFSPTD
                     LLSSSLACCMFTMMGIVAERHEMDISGSYASVEKIMESDPRRVGTIIIRMTIADRELS
                     EKQKEILEHTARTCPVLLSLSGEMVKELDLSFEAAAAID"
     gene            4328..5116
                     /locus_tag="PK742_07950"
     CDS             4328..5116
                     /locus_tag="PK742_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76564.1"
                     /translation="MRKVGLFGVLVLAGMLVFGQTGPVVINQAQSAGRPGGLVYNKEL
                     TFGGILTTNGWGVNSTYARRSSVSVQRLFFTELMFIHHPKELKKVNEYNFSFSYSAPK
                     PFVYGKQNSFFSWKAGYGQQFLIGEKAEKSGFEVSFKYLAGASLGLLKPYYLEILYEE
                     DGTVFKVSQQYGPATEDLFLDATSIYGYSGFKKGLSEISVVPGGFGRAGMLFDWASYD
                     EYVKSLEAGIGAELYPKDIPIMILENNKPYFFYLYLSLQLGKKW"
     gene            5126..6010
                     /gene="lipA"
                     /locus_tag="PK742_07955"
     CDS             5126..6010
                     /gene="lipA"
                     /locus_tag="PK742_07955"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQU76565.1"
                     /translation="MLELNVIGQNTADEPRKRKPSWLRVKLPTGENYKKVRQLVDNYQ
                     LHTICESGNCPNMGECWGEGTATFMILGNVCTRSCSFCAVATGRPPEYDQEEPQRVAE
                     AIDLMGVKHAVITSVNRDELPDSGAEIWYQTVSAVKTRTPHVTIETLIPDVKSRWEAL
                     ERMISGGQEVVSHNMETVERLYRLVRPQARYNRSLEQIRRTKEYGKRTKSGIMLGLGE
                     SQEEVHKSMDDLVAHGCDVLTLGQYLQPTRMHLDVKAFVTPDEFEAYKEEGLRRGFLY
                     VESGPLVRSSYHAERHLI"
     gene            6090..>6889
                     /locus_tag="PK742_07960"
     CDS             6090..>6889
                     /locus_tag="PK742_07960"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQU76566.1"
                     /translation="MKLRSTIFPLMLAGATIAVLALMNQTQKPAASAVGNTEEVEEEH
                     EQALIQAYYDYMFELQKNPFTGELTFADVYAGRMEVLEKQAASTRGGLLEWEFMGPNN
                     VGGRTRSILIDPTNPSRMYAGSVSGGVFITDNGGLSWYPSPGTETMENINISSMARTA
                     NGDIYIGTGEDFLVGIPPFDGTSSETHRFTGNGMYKSTDGCQTFTQLSATEPTPGVLG
                     TSSGVDWAYVYRIATNSSDANLVVAGHNGGLQYSTDGGTTWSYCTNTTL"
BASE COUNT         1787 a         1589 c         1692 g         1821 t
ORIGIN      
        1 aaatattttg gtaggggctg ttgccctgac cagcaggtgt ccggtaaata cgttattgct
       61 ggtctctatg cgaagcggaa aatacagctg gtcaataagc acccctccgg cagtaatatc
      121 tgcagtcagt tcttcatact gaacttttct gccattgccc atccagccca gatctccgcc
      181 aatggaaatg ggtatgtcag gagaaggctg atacagaaag ttgaccatga cgccaaccag
      241 ggcatcttta ttaacttcct tgaattcatc ctgcggcagc ccgatcacca ggtcaacgcc
      301 aaacaatccc tggccattgg tctgtgcgat caatgcaaca ggagaaagca ataaaagaga
      361 aaggagtagt cttgaatgca tatgtgagtt ttaatatgtc agtccaaatc ttatgccata
      421 cctgactatt tcctgtaggc atggaacagg cttttccacc tgatagacaa aactcccagc
      481 gcagcttcct tcacgatgcc ttttgacatc ttggagctgc ctttcaccct gtctttaaaa
      541 gtaatgggaa tttctctgat acggaaaccg gagcgccagg cggcatattt catctcgatc
      601 tggaaggcat aaccaatgaa gcggatcttg tccagatcta ttgcttccag cacttcccgc
      661 ttgtagcata caaaaccggc agtggtatca cgaacgggca tccaggtgat caatcgcaca
      721 taaatactgg cacctttgga tatcaggaca cggtcggccg gccagttttc tactcttccg
      781 cccttcacat atctcgaacc aactgataca tcggcacctt cagaacaagc tttgatcagt
      841 ctgggcagat ctttcgggtc atgtgaaaag tcagcatcca tttccagcac gaaagaatac
      901 cctctttcca gtgcccatct gaaacccaga atataagccg tgcccaatcc ttgtttgccg
      961 ggtctttcca acaggtgaag ccggtcagga tattgcttca tcaggcctct gaccgcatcg
     1021 gcagttccat caggagagcc gtcatcaaca ataagcaatg cataggaggg atcctgatcc
     1081 aatacagccg ggatcataag accgatgttc tcgacctctt tgtaggtagg tatgataatg
     1141 aggctgctgg tcaagatcac ttgtttttct gctgaagacg catgcggatc atcttcagct
     1201 taagcttggt atcaagcgga aattcatttt gcagaatcca gtcaaaatag tgaggttctt
     1261 tcttgtagat ctcttccacc atgcggtcct tgtatttgcc aaagttgaag tattccttcc
     1321 cgtctttaaa gaccattctt ctgccggtat ctacaaaatc tccgtctttt gtgaattcat
     1381 gcagaaagga aacgttattc tcaagctgat ctccataacg ttccagttgc ccgttcagta
     1441 cctcgaaggt ggctatcaca tctgcttcgg ctgaatgggc attgaccaat tccttgttgc
     1501 agaagaatcg ataggcggtc tggagatcac gcttttccat cagagtaaag atgcgcatca
     1561 catccacata ttttcggttg gtatcaaaac ccacatctgc acgaaggaac tcttctatta
     1621 gcagtggtat atcaaattta ttgctgttat atcctgccat atctgcattg ccaataaaat
     1681 cacggatctc aggtgcgatc tttctgaagg agggcttatc cttcacatct gcatctgaga
     1741 ttccatgtat catggttgat tctgcaggta taggtatgcc cgggttgacc cggtctgtgc
     1801 ggatctcctg ttgctgatcg gggtacagtt tcagcatact gatctcaatg atccggtctg
     1861 tagcaatatt gacaccggtg gtttccagat ctaaaaaaac gaggggtttg tcaagatgaa
     1921 gcatgtcctg caaaaatcaa actaatccat caatattcaa acctgcaaaa tgtcctgaac
     1981 tcatcagaag gagtatgtcg aatgaacttg cctgtaaatg ccgtgtcatt tcctgcagat
     2041 cgttacacac caggagaccc ggatcctgaa agaaatcctg tacttcttct ttgcttaccg
     2101 gtggaagctt tttcatttcc agagtatgcg gatcaaagaa caccacacgg atgtctgcag
     2161 catccagagc atgtgcatac tgtggcagaa agcctttgtt cagactgctg aaggtatgca
     2221 gttcaagaca ggctgtaatg cgctgtgtcg gatacatctc tctgattgcc gaggtggtag
     2281 cctttacctt ggagggagca tgggcgaaat ctcgaaatac agttttaccc ttgcgctcat
     2341 atgctttctc cattcttctg gctgctcctg taaaatcaga caagtgcccg agaaagctgg
     2401 aggtgtcaat tcctgccaga ccggccagag tggcagctcc gctcaggttg ctgatgttgt
     2461 gtttgccgaa gactgatatc gggtaagatg ttccgtcata ttctattgta gtgcctgata
     2521 cgtccgtatg atacctgaaa ggagtgtacc agatcacctg ccggggagcc acttcttcct
     2581 gcaccagtct gaccagtacc tcatcttctg catttgccac cagcttatct gccggttgca
     2641 tttgtcgcag cagcttgcgg aatgtcatca gataatcatc aaaagtgggg aatacattga
     2701 aatgatccca tgcgatacca ctgatcagga gtatctgggc tttatagaac agaaattttg
     2761 gttcaggtaa cagggggctt gcgtgatact catcgccctc tatgatcatc agcggagcct
     2821 gactgagcct gaccatggtt tgaaatcctt ccagctgtgc accaaccaga tagtcgaaat
     2881 gtgatgctgc ggcccgaaga ccgtgcatca gcatagaggt gatggtggtc tttccatgac
     2941 tgccgcatat agccattctg acagcttcgg ctgatacact gtaaatgaat tcaggaaaag
     3001 acatgacggg tagtcctatc ctggtagctt ccaggagctc cgggttgtca gatcgggcat
     3061 gcatgcccag tatgacccgg tcaaggtctg cggtaattcg ctccggaaac cagcccatat
     3121 gttccggtaa caatccggcc tttgccaggc ggttgaggga tggttcgaag atctcatcgt
     3181 ctgatcctgt tacctgatac cccgcctctt gcagggccag cgccagatta tgcattacgg
     3241 cccctccgat ggctatcagg tggatcttgc ctttttcttc cataggcgac aagtttaagc
     3301 agccttgtgg aaaaccattg actgtttgaa attattttgt gaaatttgcg ttaatcgaaa
     3361 agatcaaagt aaacagacac atatgttcac agtagctaaa aagtcccgtt ggatcatcgt
     3421 agccttaatt ttgtccatgg ctgttgtggg cttgtcttcc tgccacaccg aaggatgtcc
     3481 gaataagatc acccaggtga acccattcgg tgctgacgat gtttcctgat gcatgatcaa
     3541 ttggatcgac ctgaccgatg aggctgtctg gcaagatgtt gtcagaagat ctcacatcca
     3601 tcccgtttgt atttttaaac actctacacg ttgtggagta tcttcaatgg tgttaaatcg
     3661 tctggagcag catgagccgg tgcagaacag cagctggtat atgctcgata tgatcagata
     3721 ccgaaccttt tctgacctca ttgcagaatc tacaggtgtg gtgcatgaat cacctcagat
     3781 gatcatcctt tctgatggtg cggttcagat acacttttct catacggaga tcagaacaga
     3841 agtgatcagt caatggctgc agcagcttca aaggaaagat ccagttcttt gaccatctca
     3901 cctgacaggc tgagcagtac cggacaggtt ctggcagtat gttccagaat ttccttttgt
     3961 ttttcgctga gctcccggtc tgcaatggtc atgcgtatga tgatcgttcc cacacgccgc
     4021 gggtcacttt ccatgatctt ttccacactg gcatagctgc cactgatgtc catctcatgg
     4081 cgttctgcca caatacccat catggtgaac atgcaacagg ccagtgacga agacagcaga
     4141 tcggtaggag agaaggcttc ccccttgcca tggttatcaa ctggagcatc tgtctgtatc
     4201 cggctgcctg aagccaggtg tacagcttcc gtgtgcaact tacctgtgta ggtcactttt
     4261 gaaacaatca tgacgtaaaa gacgtttgtt ccttaaagat aatccgatta ttttcgttga
     4321 gatttacatg agaaaggttg gtctatttgg ggtgcttgtt ttggctggta tgctggtttt
     4381 cggacagacc ggcccggtgg tgatcaatca ggctcagtct gcaggaagac cgggtggtct
     4441 ggtctataat aaggagttga ccttcggagg tatcctgacc accaacggtt ggggggtcaa
     4501 cagtacctat gccaggcgct catctgtaag tgtacagagg ctttttttta ctgagctgat
     4561 gttcatccac catccaaaag agctgaagaa ggtcaatgaa tacaattttt cctttagtta
     4621 ttcagctcca aagccattcg tttatggcaa acagaacagt tttttcagct ggaaggcagg
     4681 ttatggccaa caatttctga taggggaaaa ggcagaaaaa agtggatttg aggtcagctt
     4741 caaatacctt gcaggagctt ctctgggcct gctgaaaccc tactatctgg agattctgta
     4801 tgaagaagat ggtactgtgt tcaaggtgtc acaacagtat ggcccggcaa cagaagatct
     4861 ttttctggat gcaaccagta tttacggtta cagcggattt aagaaaggcc tctctgagat
     4921 cagtgtggta ccgggcggat ttggccgtgc aggaatgttg tttgactggg cctcatatga
     4981 tgaatatgtc aaatctctcg aagcaggtat aggggctgaa ctgtatccaa aagatatacc
     5041 gatcatgata ctggagaaca acaaacccta ctttttctat ttatatctaa gtttgcagct
     5101 aggtaaaaag tggtgaaaga gcggtatgct tgaattgaat gtgatcggcc agaatactgc
     5161 cgatgaacca agaaaacgca agccttcctg gctgcgggtg aaacttccaa ccggagagaa
     5221 ctataagaag gtcagacaac tggttgacaa ctaccagttg cataccatct gtgagagtgg
     5281 taactgcccg aatatgggtg aatgctgggg tgaaggaaca gccaccttta tgatactggg
     5341 caatgtttgc acccggtcat gctcgttctg tgctgtggct accggcaggc ctccggaata
     5401 cgaccaggaa gagcctcagc gggtggcgga agccattgac ctgatggggg tcaaacatgc
     5461 tgttattacc tccgtgaacc gggatgaatt gcctgacagt ggcgcagaga tctggtacca
     5521 gacggtcagt gccgttaaaa cacggactcc ccatgtcacg atagaaaccc tgattccgga
     5581 tgtaaagtcc agatgggagg ctctggagcg gatgatcagc ggcgggcagg aagtggtaag
     5641 ccacaatatg gaaacagtgg aacgcctgta cagactggtg aggccacagg caaggtacaa
     5701 cagaagcctg gaacagatca gaagaaccaa agagtacgga aagcggacta aaagtggcat
     5761 tatgctgggg cttggcgaat cacaggagga ggtgcataaa agcatggatg atctggtggc
     5821 tcatggctgc gatgtcctaa ctttaggtca gtatcttcag cccacccgca tgcacctgga
     5881 tgtgaaggcc tttgtaacac ctgatgaatt cgaggcttac aaagaagaag gtttgcgcag
     5941 aggctttttg tatgtggaaa gcggaccgtt ggtcagatct tcctaccatg cggaaaggca
     6001 cctgatctga gaggtgtaaa aaatgactta actgttaatt tatcttaaaa gaaaatcact
     6061 aatttcaccc gaaaataaat tccatttaga tgaaactgag gtctactatt tttcctttga
     6121 tgctggcagg agcaaccatt gctgtcctgg cgctgatgaa tcagactcag aaaccggctg
     6181 cttcagctgt gggtaatact gaggaggtag aagaagagca tgagcaggcg ttgattcagg
     6241 catattacga ttatatgttt gaactgcaga agaatccctt tactggcgaa ctcacctttg
     6301 cggatgtgta tgctggtcgt atggaggtgc tggaaaagca ggcagcttct acacgaggtg
     6361 gcctgctcga atgggaattc atgggtccca ataacgttgg tggacgcacc aggtctattt
     6421 tgatagaccc gaccaacccc agccgcatgt atgcaggcag tgtgagcggt ggtgtgttca
     6481 ttactgataa cggtggcctc agctggtatc cttctcctgg aacagagacc atggagaata
     6541 tcaatatcag tagcatggcc agaactgcca atggagatat ctatattggt acaggtgaag
     6601 atttcctggt tggtattcct cctttcgatg gtacatctag tgaaactcac aggtttaccg
     6661 gtaatggtat gtacaaatca acagacggct gtcagacatt cacacagctt agtgcaacag
     6721 aaccgacccc cggggtactt ggaacttcct ctggagttga ctgggcttat gtgtacagga
     6781 tcgccaccaa ttcaagcgat gccaatttgg tggttgccgg acacaacggc ggtctgcagt
     6841 acagtactga tggcggaaca acctggagct attgtaccaa cacaactct
//
LOCUS       DAOMAX010000134         6838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1706_length_6838_cov_4.318738, whole genome shotgun sequence.
ACCESSION   DAOMAX010000134 DAOMAX010000000
VERSION     DAOMAX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1706_length_6838_cov_4.318738"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK742_07965"
     CDS             complement(<1..599)
                     /locus_tag="PK742_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQU76567.1"
                     /translation="MSGKTCSTFGAMRTSEKQAMLLLHLCVFIWGFTAILGNLITLRE
                     TVLVWYRMGITSLSLLFLPAFWRGIRTIRKQDAWRIASVGIMVSVHWVTFYGAIKYAN
                     VSVALTCLATVSFFTSLLEPLFFRSRHRFTEILFGLAVIPALYMVFRFTGSYATGMIM
                     GVFSAFLAAVFTILNKRLTVSYDPFSITFLELATGFVFLG"
     gene            686..2371
                     /locus_tag="PK742_07970"
     CDS             686..2371
                     /locus_tag="PK742_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76568.1"
                     /translation="MNRILFLTLIMSLSAAMYAQFDRMVLLEEFTQASCGPCASQNPD
                     FNALLQENTDIATSIKYQVWWPGYDPMYEHNTSDVDTRVDYYGITGVPHALMDGVNIA
                     NDCGYYVGAPACVSQTDIDLASASSSPFLIELSHVVSDNYDHIDVTMHITAGADVSGA
                     LSAHIVVVEEEVIFDNPPGSNGEKEFYNVMKKMLPSANGTTLSDTWAPGDEITLTESW
                     NFANVYDPSEIAVVAFIQDNATGMVYQAAYSAPYPVYLNDVLNVSSNLTGSNNRYLCD
                     GNTSPLVTVKNTGEEVLNSLTISYSVNGEDQEPYSWSGSLPFGERETISLPMISFLLN
                     DENELAVTLSEPNGTADDFDNNNESTAIALAAPESPTNTIHLKLRTDNYASETTWQVI
                     NEDGDVMAEGGPYNGQNNQVVFDNDIALPASGCYDFIIYDEYGDGICCTYGSGYYEIT
                     DTDGAMLVEGGAFGSEDLAIVKVTDVVGTDEIPALEWLMYPNPATDLVQLQLPGNSHY
                     LLEITDMTGRPVLTERSYSSGNHTLHTENLAPGMYQVILRSDLQISSQLLTIQ"
     gene            2477..4399
                     /locus_tag="PK742_07975"
     CDS             2477..4399
                     /locus_tag="PK742_07975"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HQU76569.1"
                     /translation="MKKALLLLLVASGSVAMAQITALSEDFEGGVLPDLWWQETAATD
                     GGWLVGDADFQSSSAWPVEEHTVMIATNDDACNCNKLDDLLSTPSLSLVGMTSPYLVF
                     DYYFGEFTYSGATEDLSVDVSLDGGINWTELVNLDGAGGSLYWRTASIDLGAYAGMSD
                     VMIGFRYTDGGGWLYGAAIDDVKVYEPSPVDVELSALTFGQFQEVGTGVGITGTITNI
                     GLDAITSVDITWTDGGTTETFTLDGISIPAFGTYNFTHEVDYVPASAISYDIDVELSE
                     PNGVVDPTPENNTKGSVVSGCSYIPPKHMVAEEATGTWCGWCPRGAVFMDIMTEDFPE
                     DFIGIAVHNSDPMEVTEYDNGVGAFPGFSGYPSVIIDRNEIVDPSQMPDVIDTRLADI
                     APVDMTIDLTYDEASHSISAEVTAEFVTQLSGINYRLNLVLTEDNVTGTGSGYAQTNY
                     YAGGGYGPMGGYEDLPGTVPAADMIYQHVARGLASGWNGTSGSVPADVNAGDMASHTY
                     EWDLDADWNASEMHAIGMIIDQNSGKILNAVSTKVSNSQPEAIEDFPGLAGLEVYPNP
                     SSNVAYVRVNLENTMDVTMRITDINGQVMMARNYGNLYGDMLLTVPVSDMPAGMYLVE
                     IISGAYQTTTRLMVAH"
     gene            complement(4448..5146)
                     /gene="radC"
                     /locus_tag="PK742_07980"
     CDS             complement(4448..5146)
                     /gene="radC"
                     /locus_tag="PK742_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQU76570.1"
                     /translation="MPYDEKNYTIKQLSEEDRPREKLLNQGRQRLTNAELMAILIQSG
                     SDRETAIELAQRILLSCNNNLQELGRLEVADLCAFRGIGPAKAITIVAALELGRRRQL
                     SEAEDRIQIRSSRDIYAWMEPLLADLSVEEFWALYLNRGNRIIGKERMSQGGVSGTVV
                     DVKVLLRNAVQRLCSAVVVVHNHPSGTLQPSQADIQLTKKLKQAAELMDIRLIDHLII
                     AGKGYYSFADEGML"
     gene            complement(5224..5472)
                     /gene="rpsT"
                     /locus_tag="PK742_07985"
     CDS             complement(5224..5472)
                     /gene="rpsT"
                     /locus_tag="PK742_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQU76571.1"
                     /translation="MAHHKATKKSIRQSAKRRLSNRYYGKTTRNAVRKLRATEDAGEA
                     KQSLPEVISMIDKLAKRNIIHKNKAANLKSKLMKKVNA"
     gene            complement(5579..>6838)
                     /gene="hutH"
                     /locus_tag="PK742_07990"
     CDS             complement(5579..>6838)
                     /gene="hutH"
                     /locus_tag="PK742_07990"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQU76572.1"
                     /translation="QRNLILSHACGTGPELEAETIRYLLFLKIQSLSYGYSGVAVETV
                     QRLIDMYNADVLPVIFEQGSLGASGDLCPLAHMSLPLIGEGEVRYKGIRRDAAGVLDE
                     LGWKPLQLRSKEGLALLNGTQFMNAHAVRCIAAAERLLQLADLTGALSFDAWDCKTEP
                     FDERIHRIRNQAGQQQAAANLRSILQGSEISTRPKVQVQDPYSFRCMPQVHGASRDAV
                     AYCRDIVSREANGVTDNPNIFADGDAILSGGNFHGQPLALSLDFLAMAVSELASISER
                     RVYRLLSGSRDLPEFLVEGSGLHSGLMIPQYTAASLVSQNKQLCTPASVDSIPSCNEQ
                     EDHVSMGANAATKCLRVIQNTTRVLAIEWLTAAQALEFRRPLKTSPVLEGLMATYRQQ
                     VSFNQADRFLHPDIMQTVSFLDSIELQ"
BASE COUNT         1752 a         1739 c         1621 g         1726 t
ORIGIN      
        1 cccagaaaaa caaaccccgt tgccagctcc agaaacgtaa tggaaaaagg atcatatgat
       61 accgttagtc ttttgtttaa gatggtgaaa acagcagcca gaaatgctga aaaaacaccc
      121 atgatcatac cggtagcata gctgcctgtg aaccggaaca ccatatacag cgcaggtatg
      181 acagcaagcc caaacaggat ctctgtaaac ctgtgccggc ttctgaaaaa aagcggttcc
      241 agcaacgatg taaaaaatga tacagtagcc aggcatgtaa gcgccacact tacattagca
      301 tacttaatgg ctccataaaa ggtaacccag tgcacagata ccatgatccc tactgatgct
      361 atacgccacg catcctgttt tctgatcgtg cggataccac gccagaaagc cggcagaaaa
      421 agtaaagaca gactggtaat gcccattctg taccagacaa gcaccgtttc acgtaacgtg
      481 atcagattgc ccaggatggc tgtaaacccc catatgaaaa cacagaggtg cagcaacaac
      541 atggcttgtt tctcagaggt tcgcatggcg ccaaaagtag aacaggtttt gccactcatt
      601 caaatgtcag agaattttca atttctgcac taagtcagat ggatcggcca ggtctatacc
      661 tacctttagg agataatatc acaaaatgaa cagaatactt tttcttaccc tcattatgag
      721 cctttcagca gcaatgtatg ctcagtttga ccggatggtt ctgctggaag aattcaccca
      781 ggctagctgc ggaccctgtg cctcacaaaa tcccgatttc aatgccttgc tgcaggaaaa
      841 cacagacata gccacatcca tcaaatacca ggtgtggtgg cccggatatg accctatgta
      901 tgaacacaat acaagcgatg tggacacacg ggttgattac tacggtataa ccggtgttcc
      961 tcacgctctg atggatggag tgaacatagc aaatgactgt ggctattatg taggtgcccc
     1021 cgcctgcgtc agtcagacag atattgatct ggcttctgcc agcagttctc cctttctgat
     1081 agaattgagt catgtggtgt cagacaacta cgaccatatt gatgtgacca tgcacatcac
     1141 agccggtgca gatgtttccg gtgcactcag tgcacatatc gtagtggtgg aagaagaagt
     1201 gatctttgac aatccgccag gctcaaacgg tgaaaaagag ttttacaacg tcatgaaaaa
     1261 aatgctgcca tcagccaatg gcaccacact gtcagatacc tgggcaccgg gcgatgagat
     1321 caccctgacc gaaagctgga attttgccaa tgtgtatgat ccgtctgaaa ttgctgtggt
     1381 ggcattcatt caggacaacg ccaccggaat ggtgtaccag gcagcataca gtgcccctta
     1441 ccccgtgtac ctgaatgatg tgctgaacgt gagcagcaac cttacaggca gcaacaacag
     1501 gtatctgtgt gatggtaata ccagcccttt ggttaccgtc aagaataccg gagaagaagt
     1561 actcaacagt ctgaccatca gctactcagt gaacggcgaa gaccaggagc catacagctg
     1621 gtcgggaagt ctgcctttcg gcgaacgtga aacaatcagt ctgcccatga tcagttttct
     1681 cctcaatgat gaaaatgaac tggcggtcac cctcagcgaa ccaaatggta cggccgatga
     1741 ctttgacaac aacaatgaaa gcaccgccat cgctctggca gctccggagt cacctaccaa
     1801 taccattcac ctgaagctcc gcacagataa ctatgccagt gagaccacct ggcaggtgat
     1861 caatgaagac ggagatgtca tggcagaagg cgggccctat aatggtcaga ataatcaggt
     1921 tgtatttgat aatgacattg ctcttcccgc atcaggttgc tacgacttta tcatatacga
     1981 tgaatatggc gatggcatct gctgcaccta tggctccggt tactatgaga tcacagatac
     2041 agatggtgcc atgctggtag aaggtggtgc ttttggctca gaagatcttg ccattgtcaa
     2101 agtaaccgat gttgtaggca ccgatgaaat tccggcactg gaatggctca tgtatccgaa
     2161 ccctgccacc gatctggtac aattacaatt gcccggaaac agccattacc tgctggaaat
     2221 aactgatatg acaggccggc cggttcttac cgaaagatct tatagttcag gtaaccacac
     2281 actgcacaca gagaatctgg caccgggtat gtatcaggtc atcttacggt cagatttaca
     2341 gatcagcagc caactgctca ccatacagtg aaatgctgct ccaaaactgg ttgatcaggg
     2401 ccttcgggcc ctttttttat gtaaggaaaa gagtatcttt aacctctaac aaatttctta
     2461 ttaagcaaat actacaatga aaaaagcttt actcctgttg ctggtggctt ccggatcggt
     2521 tgccatggcg caaatcaccg ccctctcaga ggattttgaa gggggggtat taccggatct
     2581 ttggtggcag gaaactgctg ctactgatgg tggctggctg gttggtgatg cagactttca
     2641 atccagttct gcatggccgg ttgaagaaca taccgtcatg atcgccacca atgatgatgc
     2701 ctgtaactgt aataagctgg acgatctgct gtcaactcct tccttaagcc tggttggcat
     2761 gaccagccct tacctggtat tcgattacta tttcggtgaa ttcacctatt caggtgcaac
     2821 agaagatctg tctgttgatg tatcactgga tggtggtatc aactggaccg aactggtgaa
     2881 tctggatggc gcaggtggat cactatactg gcgcaccgct tctattgacc tcggtgccta
     2941 tgcgggcatg agcgatgtca tgatcggttt ccgctatact gatggcggtg gctggttgta
     3001 tggtgctgcc attgatgatg tgaaggtata tgaaccttct cctgttgacg tagaactgag
     3061 cgcccttacc ttcggacaat tccaggaagt tggtacaggt gttggcatta ccggaaccat
     3121 taccaacatt ggtctggacg ccattacctc tgttgacatt acctggaccg atggcggtac
     3181 tacagaaacc ttcacactgg atggtatcag tattcctgct tttggcacct acaacttcac
     3241 acatgaggtg gactatgtgc ctgcttctgc tatttcctat gatatcgatg tggaactgag
     3301 cgaaccgaac ggagttgttg accctactcc ggaaaacaac accaaaggtt ctgtagtgag
     3361 cggttgctcc tatattcctc ctaaacacat ggttgctgaa gaagcaaccg gtacctggtg
     3421 cggctggtgc ccacgcggtg ctgtgttcat ggatatcatg acagaagatt ttccggaaga
     3481 tttcattggc attgctgtac acaacagcga cccgatggaa gtgaccgaat acgataatgg
     3541 tgtgggtgca ttccccggct tcagcggcta tccttctgtg atcattgatc gcaatgaaat
     3601 tgttgaccct tcacaaatgc ctgatgtgat cgacacacgc ctggctgata ttgccccggt
     3661 tgatatgacc attgatctga cctatgatga agcatctcac agcatcagtg ctgaagtaac
     3721 tgctgaattc gttacccagc tgagcggcat caactaccgt ctaaacctgg ttctgacaga
     3781 agacaatgta accggaaccg gttccggcta cgcccagacc aactattatg caggtggtgg
     3841 ttacggccct atgggaggat acgaagacct gcccggaact gtgcccgctg ctgatatgat
     3901 ctaccagcat gtagcccgcg gccttgccag cggctggaac ggaacctccg gttctgtacc
     3961 ggcagatgtg aacgccggcg atatggccag ccacacatac gagtgggatc tggatgccga
     4021 ctggaatgca tcagaaatgc atgccattgg tatgatcatt gaccagaact caggaaagat
     4081 cctcaatgcg gtttctacta aagtgagcaa ttcacagcca gaagctattg aagacttccc
     4141 cggccttgca ggtcttgagg tttaccctaa cccatccagc aatgtagctt atgtaagggt
     4201 caacctggaa aacaccatgg atgtgaccat gcgtatcacc gacatcaacg gtcaggtgat
     4261 gatggcccgc aactatggca acctgtacgg cgatatgctg ttgactgtgc ctgtatctga
     4321 tatgcctgcc ggtatgtatc tcgtagagat catcagcggt gcttatcaga ctacaacccg
     4381 tctgatggtt gcacattaat aagaactctg ttttgcaaaa aaagcccccg gcatgccggg
     4441 ggctttttta taacatgccc tcatctgcga aactgtaata tccctttcct gctatgatca
     4501 gatggtctat caaacgaata tccatcagct ctgcggcttg tttcaacttc tttgtaagct
     4561 gaatatcagc ctgactgggc tgtaacgttc cgctggggtg attatgtact accactactg
     4621 cactgcacaa acgctgcact gcattcctga gcagtacctt tacatctaca actgtgccgc
     4681 tcacgcctcc ctggctcatg cgctctttac ctatgatacg gttaccacgg ttgaggtata
     4741 gtgcccagaa ttcttcaact gaaagatctg ccagcagcgg ctccatccag gcataaatat
     4801 cacggctgga gcgtatctga atgcggtctt ccgcttcact gagctgcctc cttctgccta
     4861 actcaagtgc cgctactatg gttattgcct tggccggacc aatacccctg aatgcgcaga
     4921 gatctgccac ttccagcctt cccagttcct gcaaattgtt gttgcacgat aatagtatgc
     4981 gttgcgccag ttctatggcc gtttccctgt cactccctga ttgtatcaga atggccatca
     5041 gctcagcgtt ggtcaatcgc tgccggccct ggttaagcaa tttctctctt gggcggtctt
     5101 cttctgataa ctgcttgatc gtataattct tttcatcata aggcatgctg taaatatagg
     5161 ccttctgagc atatataaaa aaaggccggt gcgttgcacc ggcctttcag gatataaggt
     5221 ctatcaggcg ttcaccttct tcatgagctt gctcttgaga ttggctgcct tattcttatg
     5281 tatgatattg cgtttagcca gcttgtcgat catagaaatg acctcaggca gactctgctt
     5341 tgcttctccg gcatcttctg tagcccgcag cttgcgaacc gcattacggg tggttttacc
     5401 ataatagcgg ttagacaacc ttcttttggc agattgacgg atggatttct ttgtagcctt
     5461 atgatgtgcc atgtagcgtt agtttaaaac ggattgcaaa gataagcctt cccagacaat
     5521 ttcaaaagaa aaaaaacgga ttcctgccag aaaacaattg gctgaatatc tgcctaaatc
     5581 attgtaattc aatagaatcc agaaaactga ccgtctgcat gatatccgga tgcagaaacc
     5641 tgtctgcctg gttgaagctg acctgttggc ggtaggtggc catcaggcct tccagcaccg
     5701 gactggtctt gagcggcctt ctgaactcca gggcctgtgc agctgtcagc cactcaatgg
     5761 ccagtacccg tgtggtattt tgtatgaccc gcaggcattt ggtggctgca ttggcaccca
     5821 tgctcacatg gtcttcctgc tcattgcagg atggaatact gtcaacactg gcaggggtgc
     5881 acagctgttt gttctgcgat accagactgg cagcggtgta ctgcggtatc atcaggccgg
     5941 aatgcaggcc actgccttcc accaggaact ccggcaggtc tctggaacca gacaacagcc
     6001 ggtacaccct acgttctgag atgctggcca gttcactcac tgccatagcc agaaaatcaa
     6061 gcgacaaggc cagtggctgt ccgtgaaaat tgcccccgga aagaatggca tcaccatctg
     6121 caaaaatgtt cgggttatcg gtaacgccgt tggcttcccg tgaaacgatg tcccggcaat
     6181 acgcaactgc atctcggctg gcaccatgca cctggggcat gcatcggaat gagtaagggt
     6241 cctgtacctg taccttgggt ctggtgctta tttcacttcc ctgcagaatg gaccggagat
     6301 tggctgcagc ctgttgctgc ccggcctgat tacgaatgcg atgaatgcgt tcatcaaatg
     6361 gctccgtctt acaatcccat gcatcaaaag acaaggcacc tgtcagatct gccagttgca
     6421 ataatctttc ggctgcagct atgcacctta ctgcatgcgc attcatgaac tgggttccgt
     6481 tcagcagggc taatccttcc tttgaacgca gttgcagagg tttccacccc agctcatcca
     6541 gcactccggc agcatccctc ctgatccctt tatacctgac ctctccttcg ccaatcagcg
     6601 gcaggctcat atgtgccagc ggacaaagat cgccggaggc acccagtgat ccctgctcaa
     6661 atatgaccgg caatacatct gcattataca tatctataag tcgctgaact gtttcaaccg
     6721 ccaccccgct gtaaccataa gacaatgact gtatcttcag aaaaagcagg tatctgatgg
     6781 tctctgcctc cagttccggg ccggtaccgc aggcatgaga taaaataaga ttacgctg
//
LOCUS       DAOMAX010000135         6764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1741_length_6764_cov_3.792070, whole genome shotgun sequence.
ACCESSION   DAOMAX010000135 DAOMAX010000000
VERSION     DAOMAX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_6764_cov_3.792070"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PK742_07995"
     CDS             complement(<1..399)
                     /locus_tag="PK742_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQU76573.1"
                     /translation="MNKVTVIGAGNVGATVANVLAHKDFLSEVIVLDIKEGTAEGKSL
                     DSWQQAPIDYYSTRIKGVTNDYAATAGSDVVVITSGLPRKPGMSRDDLISVNAGIVKS
                     VTENVLAHSPDAILIIVSNPLDVMCYCAYLT"
     gene            506..1927
                     /locus_tag="PK742_08000"
     CDS             506..1927
                     /locus_tag="PK742_08000"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76574.1"
                     /translation="MIRKFLLPILLAAPLWMLAQENYCGTVQTDSDLAWLREWQASYN
                     GSGTRGSDLIYVPLKVHIVGNDEGGGRLTLRTLYTDICNLNEQFAPTGFYFYIFGDID
                     YINNSDYYEHDWLAGAYMFAENNVTNLVNIYFVGDPAGNCGYFSPSGNAIAVANSCAG
                     TSNTTIAHELGHFFSLPHTFYGWEFGLPDIDDQEQVDGDNCTTAADGFCDTPPDYLPE
                     RWSCFTLPVFTDPDGVEFSPDETLYMSYSSDECQNRFSEEQQEAMIDNINGPRAGLLT
                     WEPISVADVESAELLSPEDNITNLFSNYTELSWSSVENALGYELIVSLTPTFTAISAN
                     IFTTDTTVILTELAPEKTYRWKVRVIAEGNTCEDYTEYRTFSTGAETLANDLTDLESD
                     GWMVFPNPVAAGSGLMVKAENTWQSGDLLQVFNNGGQLIWQEQPGVGAQQHMIATNNW
                     PTGMYLVRMTMDGLVSSSPVFVQ"
     gene            complement(1939..3309)
                     /gene="tilS"
                     /locus_tag="PK742_08005"
     CDS             complement(1939..3309)
                     /gene="tilS"
                     /locus_tag="PK742_08005"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQU76575.1"
                     /translation="MEELKNKVRQFTARQQLWLPGQQLLLALSGGMDSMLLLHYLLEE
                     GIRPGLAHVNFQLRGSDADNDQSFVASVAASLQLPLYVHTENTAAFAAKEHMSLEEAA
                     REIRYRFLYHIAYAHGYQRILTAHHARDHAETILLHQVRGSGARGLSGIPVHNQMVVR
                     PFLCLRKAEVIHWSEAVGLTYCEDRTNSDTRFARNLIRHEVLPILEKLNPGLEETLLE
                     NGRIQRELQLLADQEADRVLKKVCIRKGDDLYIGKKALLLHPAARTILFRLLRPYGFT
                     AAQLDDILQNHTHSGRLQEAGRFRLITDRTHWILTTERNNQFSWHLIREDDRHVHLPG
                     MRLELRYLTELPSSWNMSAERVYLRADDLSYPLLIRPWKQGDYLYPFGLYKPSGKPAR
                     KKVSDLLTDKKLSLPEKERVLVLCSADRILWILGIRQDERTRITSGTGRILKIKMLPA
                     RGSMYY"
     gene            complement(3313..3648)
                     /locus_tag="PK742_08010"
     CDS             complement(3313..3648)
                     /locus_tag="PK742_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76576.1"
                     /translation="MNRLEQLMDFYRENPDDPFVRYCIALEHIKSDDPGEAREWFEAL
                     INSHPDYVATYYHYGKLMESLQDKDAAVSLYQAGIEVASKAGDLHALSELQNARNEVV
                     NAFDIEFDD"
     gene            3724..4464
                     /locus_tag="PK742_08015"
     CDS             3724..4464
                     /locus_tag="PK742_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQU76577.1"
                     /translation="MKILVCISKVPDTTAKISFTDNNTKFNEDKVQFIINPYDEWYAL
                     VRALELKEGAGGTVTIVNAGPADNEQIIRKGLALGADDAIRIDMQANDAFQVAAEIAA
                     VAKEGSYDLILCGKETIDYNGGVFGGLLAEMLSMPYVSLASKLDLQENSLLLEREIEG
                     EKEVIEVAMPCVVSAQKGMAEARIPNMRGILASKSKPVAVITPSGTTARVPVASFSLP
                     PEKGACKMVDADQAEELIRLLHEEAKVI"
     gene            4489..5454
                     /locus_tag="PK742_08020"
     CDS             4489..5454
                     /locus_tag="PK742_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQU76578.1"
                     /translation="MSVLVLAECANGKLKKNAFEAAAFASGLAAQTGADLVAVVLGSA
                     SADDLASLGKAGVARVLHASDSRLDRMTSTSYTKALQSAADAAGASIIVLSHSSVGKT
                     IAPRIAVRRNMTLVPGVSALPENGTFRKSMFSGKAWGDLKPEGDQLVITVNPNTTSFT
                     LGDGVATVESLAVELSDSDFDYKIKEVVNESAGMTPLPEAELVVSAGRGMKGPENWGI
                     IEELAGELGATTACSRPVADIGWRPHHEHVGQTGIAIKPNLYIAIGISGAIQHLAGVN
                     QSKCIVVVNKDPEAPFFKAADYGIVGDAFEVVPKLTEAVRKLKNS"
     gene            5494..6090
                     /locus_tag="PK742_08025"
     CDS             5494..6090
                     /locus_tag="PK742_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQU76579.1"
                     /translation="MRKIELEITGLSHTITQSQSYAVLLAEVDGLRKLPIVIGAFEAQ
                     AIAVAIEKLAINRPLSHDLIKNILDAFSIQLKEVLIDNLQDGIFYAKLICEQNGEVID
                     IDSRTSDALALAVRFQCPIYTYEFILDTAGTIISEEESGDDEDDADNDEEQTVTVGAS
                     DEFGNMTDDELQSALDEALASEDYEKAARIRDELARRD"
     gene            6111..>6764
                     /locus_tag="PK742_08030"
     CDS             6111..>6764
                     /locus_tag="PK742_08030"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HQU76580.1"
                     /translation="MDILRGILGLAFLVGVCVLFSKDRKAIDWKLVISGLGLQVIFAI
                     LVLRTPFVYQGFQWVSNFFVQIIQFTDAGASFVLGNWPASTQVIDGDANTIVSVGFIF
                     IFKVLPTIIFFSALTSLLYYLGILQKIVFAFAWIMSKTMRMSGAESLSAAGNIFVGQT
                     EAPLLIKPYLASMTKSEIMCVMTGGMATIAGGVMAAYVQFLGGADIEQQRIFATHLLT
                     "
BASE COUNT         1748 a         1587 c         1663 g         1766 t
ORIGIN      
        1 agtcagataa gcacaatagc acatgacatc aagcggattc gaaacaatga tcagaatggc
       61 atctggagaa tgcgccagta cgttttctgt gacagatttg actatacctg cattcacact
      121 gatcagatca tcccggctca ttccgggctt cctcggaagt cctgatgtga tgaccactac
      181 atctgaaccg gcagtggcgg catagtcatt ggtaaccccc ttgatccggg tgctgtaata
      241 gtctatgggt gcttgttgcc atgaatcaag gctcttacct tcagcggtac cttccttgat
      301 atccagtacg attacctcac tcaggaaatc tttatgcgcc agcacattgg caacggttgc
      361 gcccacattt ccggcgccaa taacggttac tttgttcatg gaaacgtcaa ttttgcggca
      421 aagttaagga atattgaatc cccccatgtc atagacacag aacgtttttt gacttaaagc
      481 gttatctgaa ataactttgc aacttatgat cagaaaattt ctcctcccga tcttgcttgc
      541 cgcaccgctt tggatgctgg cacaggaaaa ctattgcgga actgtacaga cagattcaga
      601 tctggcctgg ctgagagaat ggcaggcatc ctacaacgga tccggtacca gaggttccga
      661 tcttatttac gtaccactga aagtccatat tgtaggtaat gacgaaggtg gcggaaggct
      721 gacccttaga accttgtaca ccgatatctg caatctgaac gaacagtttg ctccaaccgg
      781 tttctacttt tatatcttcg gcgatattga ttacatcaat aacagcgact attacgaaca
      841 tgattggtta gcaggtgctt acatgtttgc agaaaacaat gtcaccaatc tggtcaacat
      901 ctattttgtt ggcgaccctg ccggaaactg cgggtacttt tcaccttcag gaaatgccat
      961 agctgtggcc aattcctgtg ccggaaccag taatactacc atcgctcatg agctgggtca
     1021 ctttttcagc ctcccccata ctttctatgg ctgggaattc ggacttcctg atattgacga
     1081 ccaggaacag gtagatggcg acaactgtac gacagctgct gatggctttt gtgatacacc
     1141 gcctgattac cttccggaaa gatggagctg tttcacactg ccggtattca cagatcctga
     1201 cggggtggaa ttttcgccgg atgaaacact gtacatgtct tattcttccg acgaatgtca
     1261 gaacagattc agcgaagaac agcaggaggc tatgattgat aatatcaatg gccccagagc
     1321 cgggttatta acatgggaac ctatttctgt agcagatgtg gaaagtgcag agcttttatc
     1381 tccggaagac aacattacca acctgttttc taactataca gagctaagct ggagcagtgt
     1441 ggaaaacgct ttgggctacg aattgatagt aagcctgacc ccaactttca ccgctatttc
     1501 tgctaatatc ttcactacag atacaactgt gatcctgact gaactggctc ctgaaaagac
     1561 ctatcgttgg aaggtgcggg taattgctga aggaaatacc tgtgaagact atacggaata
     1621 tcggactttt tctaccggtg ctgaaaccct ggccaatgac ctgactgatc tggaatctga
     1681 tggctggatg gtctttccta accctgttgc agccggatct ggtcttatgg tcaaggctga
     1741 aaatacctgg caatcaggag atctgctgca ggtttttaac aatggcggac aactgatctg
     1801 gcaggaacag cctggcgttg gtgcacagca gcatatgatc gcaactaaca actggccaac
     1861 cggtatgtac ctggtcagaa tgaccatgga tggtctggtt agcagctctc ctgtgtttgt
     1921 gcagtaacag gcagatcact aataatacat gctccctctt gcagggagca tttttatttt
     1981 caggatacga ccggtgccag acgtgatgcg ggtgcgttcg tcctgcctga tgcccaatat
     2041 ccaaaggatc cggtctgcac tgcacaatac cagcacccgt tccttttcag gcagcgatag
     2101 ctttttgtct gttagcagat cagatacttt ttttctggcg ggtttaccac tgggtttgta
     2161 taagccaaat ggatacagat agtcgccctg tttccagggt ctgatgagca aagggtagga
     2221 gaggtcatct gcccgaagat atacccgctc agctgacata ttccatgaag aaggtaattc
     2281 agtcagatag cgcagttcca gcctcatacc cggaaggtga acatgcctgt catcttcacg
     2341 gatcagatgc cagctgaact gattgttgcg ttctgttgtc aggatccagt gggtgcggtc
     2401 ggtgattagt ctgaaccgtc ctgcttcctg taacctgcca ctatgtgtat gattttgcaa
     2461 tatatcatcc agctgtgctg cagtaaaacc ataagggcga agcaatctga ataatatggt
     2521 gcgggctgca gggtgtaaca gcagggcttt cttgccaatg taaagatcat cgcccttgcg
     2581 tatgcatact ttcttcagaa cccggtctgc ttcctgatct gccagcagct gcagctcacg
     2641 ttgtatccgc ccattctcca aaagtgtttc ttccagtccg gggttcagtt tttccagtat
     2701 cggcaaaacc tcatgacgaa ttagattgcg ggcaaaccgg gtatctgaat tcgtgcgatc
     2761 ctcacaatag gtaagtccaa cagcttcaga ccaatgaata acttctgcct ttctcaggca
     2821 taaaaatggc cttaccacca tctggttgtg tacaggtatg cctgataatc cgcgggctcc
     2881 gcttcccctt acctgatgca gtaatatggt ctccgcatgg tcgcgggcat gatgggctgt
     2941 tagtatgcgc tggtaaccat gggcataggc aatatgataa agaaacctgt atcgtatttc
     3001 tcttgctgct tcttccaggc tcatgtgttc ctttgcagca aaagcagcag tgttttcagt
     3061 atgcacatac aagggaagtt gcagagatgc ggccacagac gctacaaatg actgatcatt
     3121 gtctgcatca gaaccccgaa gctggaaatt cacatgagcc agccctggtc tgatgccctc
     3181 ttccagcaag taatgcagca ataacatgga atccatacca ccactgagag ccagcagcaa
     3241 ttgttgtccc ggtagccaga gctgttgtcg ggcagtaaac tgccttacct tgtttttcag
     3301 ttcttccaat ggtcagtcgt caaattcaat atcaaaagca ttgaccactt cattgcgggc
     3361 attctgcagt tcactgagtg catgcagatc gccagctttg gaagccactt ctatgcccgc
     3421 ctggtacagg gacactgcag catccttgtc ttgtaaagac tccatcagtt tgccgtaatg
     3481 gtagtatgtg gccacataat caggatggct gttgatgagt gcttcaaacc attcacgggc
     3541 ctcacccgga tcatcagatt tgatgtgttc cagtgcaatg caataccgca caaaaggatc
     3601 atcgggattc tcccggtaaa agtccatcag ctgctccagt ctgttcatct caaaggggtt
     3661 tctcctgcgg aaatgctatc tttgtggccg gttttttaca ggtaattctc aatcaataaa
     3721 tgtatgaaaa ttctggtttg tatcagtaaa gttcccgaca cgaccgcaaa gatttcattt
     3781 acagataata acaccaaatt caatgaagac aaggtgcagt ttatcatcaa tccatatgat
     3841 gaatggtatg cgcttgttcg tgccctggaa ctcaaggaag gagccggcgg aactgttacc
     3901 attgtgaatg cagggccagc cgataatgaa cagatcattc gcaaaggtct ggccttaggt
     3961 gccgatgatg ccatccgcat tgatatgcag gcaaatgatg cattccaggt tgcagcagag
     4021 atcgctgccg tggcaaaaga aggatcatat gacctgatac tatgtggtaa agaaaccatt
     4081 gattacaacg gtggcgtgtt tggcgggctg ctggctgaaa tgctcagcat gccctatgta
     4141 agcctggcat ccaaactgga tctgcaggaa aattctctgc tgcttgaacg tgaaatcgaa
     4201 ggtgaaaagg aagtgatcga agtggctatg ccctgtgtgg tcagtgcaca aaagggaatg
     4261 gctgaagcac ggatccccaa tatgcgtggt atcctggcat ccaagagcaa gcctgttgct
     4321 gtgatcactc cgtccggaac cactgcacgg gttcctgtag cttcattttc attgcctccc
     4381 gaaaaaggag cttgtaaaat ggttgatgcc gatcaggcag aagagttgat cagactgctg
     4441 catgaagagg cgaaagtaat ttaattaaga tataaaaaaa atatatttat gtcagtgctc
     4501 gttctggccg aatgtgccaa cggaaaactt aagaaaaatg cattcgaagc cgcagctttt
     4561 gcaagtggat tagccgctca gacaggagcg gacctggtgg ctgttgtgct gggttctgca
     4621 tctgctgatg accttgcttc cctgggtaag gcaggggttg ccagggtgtt acatgcatca
     4681 gattccaggc tggaccggat gaccagcacc tcttatacca aagcgttgca atcagcagca
     4741 gatgctgccg gtgcttccat cattgtgctg tctcacagct cagtaggaaa gaccatagcc
     4801 ccccgcattg cggtaagacg aaatatgacc ctggtgcccg gcgtatctgc gctgcccgag
     4861 aacggtacct tccgtaagag catgttctcc ggaaaagcat ggggcgatct gaaaccggaa
     4921 ggagatcaac tggtcattac ggtcaacccg aataccacca gcttcacttt gggagacggc
     4981 gtggcaactg ttgaatcact ggctgtagaa ctgagtgata gtgattttga ctacaagatc
     5041 aaagaagtgg ttaatgaatc agctggtatg actccactac ctgaggctga actggtggtg
     5101 tctgcaggca gaggtatgaa aggacctgag aactggggca tcattgaaga actggccggc
     5161 gagctgggtg caaccactgc ctgttcacgt ccggtggccg acataggctg gagaccccac
     5221 catgagcacg ttggacaaac cggcattgcc atcaagccaa acctgtacat tgcaataggt
     5281 atttcaggag ctatccagca cctggccgga gttaaccaga gcaagtgcat cgtggtggtc
     5341 aacaaagacc cggaagctcc attcttcaag gcggcagact atggtatagt gggcgatgct
     5401 tttgaagtgg ttccgaagct gactgaagct gtgcgaaagc tgaaaaacag ctgaatcaga
     5461 taaagggata gtgcgtatat tagcgcagtc atcatgcgca agatcgaact ggaaattacc
     5521 ggactgtcgc acaccattac gcagtctcag agctatgcgg tgctgttggc tgaagtggat
     5581 ggcttgcgca agcttcccat tgtaatagga gcgtttgaag ctcaggcaat tgcggtagct
     5641 attgagaaac tggccatcaa cagaccgctt tctcatgacc tgatcaaaaa tattctggac
     5701 gctttcagca tacaactgaa agaagtactc atagacaatc tgcaggatgg catcttttac
     5761 gccaaactga tctgcgaaca gaacggcgaa gtaattgata ttgactccag aacctcagat
     5821 gcactggcgc tggccgtcag gtttcaatgt cctatctata cttatgaatt catcctcgat
     5881 actgcaggta ccatcatctc tgaagaagag tctggcgatg atgaagatga tgccgacaat
     5941 gatgaagaac aaactgttac cgttggtgcc tcagatgaat ttggcaatat gactgatgat
     6001 gaactgcaat ctgccctcga tgaagccctg gccagtgaag attatgaaaa ggctgcccga
     6061 attcgcgatg aacttgcacg tcgtgattaa tctcccaaac acctgataac atggatatat
     6121 tacgcggtat tctgggactt gcattcctgg ttggtgtttg tgtgctgttc agtaaagaca
     6181 gaaaagcaat tgactggaaa ctggtcatca gcggacttgg cctgcaggtc atatttgcca
     6241 tactggtgct gcgcactccg ttcgtttatc agggctttca gtgggtcagt aatttctttg
     6301 ttcagatcat tcagtttaca gacgccggtg ccagctttgt gttgggtaac tggccggctt
     6361 ctacccaggt catagacggc gatgccaata ccatcgtcag tgtgggtttc atatttatct
     6421 ttaaggtgct tcccaccatt atcttctttt cagccctgac ctccttactg tattatctcg
     6481 gcatattgca gaagatcgtt tttgcctttg cctggatcat gagcaagacc atgcgcatgt
     6541 ctggtgcaga aagcctgagt gccgccggaa atatatttgt cggacagaca gaagctccgc
     6601 tgctcatcaa accttacctg gcgtctatga ccaaaagcga gatcatgtgt gtgatgacag
     6661 gtggaatggc tactattgcc ggaggggtaa tggcggccta tgtgcagttt ctgggtgggg
     6721 cagacattga acaacaacgc attttcgcta cgcatttgct caca
//
LOCUS       DAOMAX010000136         6745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1749_length_6745_cov_3.921525, whole genome shotgun sequence.
ACCESSION   DAOMAX010000136 DAOMAX010000000
VERSION     DAOMAX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_6745_cov_3.921525"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK742_08035"
     CDS             <1..498
                     /locus_tag="PK742_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQU76581.1"
                     /translation="LNNIPLASLAAILIFTGYKLAKITLFREFWSKGMDQFVPFVVTI
                     VAILFTDLLIGVCIGIGISIIFLLRTNFHSAIMIVHDGDNYLLRFRKSVSFLNKPLIK
                     KELRKIPSNSHLIIDTAMSDFIDMDVIDTVNEFLVSARDRNITVSMDKHGQRRSQAFF
                     QTNDN"
     gene            510..1151
                     /gene="can"
                     /locus_tag="PK742_08040"
     CDS             510..1151
                     /gene="can"
                     /locus_tag="PK742_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HQU76582.1"
                     /translation="MMHSTDKLLLENKAWAQEMITDDPEYFSRLSDIQTPEFLWIGCS
                     DSRVPANQITGTQPGEIFVHRNIANMVVHTDLNLLSVLEYAVVYLKVKHVIVCGHYGC
                     GGVKAAMDSHSYGMINKWLRNIKDVYRYHRDELAGIEDQEQRTNRLVELNVMEQVMNL
                     AKTSIIQKAWSEEQRPDLHGWVYGLHDGLIKTVYDMPAGTHLDPLYEFDNLKQ"
     gene            complement(1197..1583)
                     /locus_tag="PK742_08045"
     CDS             complement(1197..1583)
                     /locus_tag="PK742_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76583.1"
                     /translation="MNRQIFLWGLLSGLMSTLAGFIWDRVYFFAMMADFSRVINLWTI
                     LAASMIGTMVAVAGFAILNRWMKKGRDITFNLLLSVITIASLVLPLSYALPLDIDFPE
                     VFPALALPMHFFPAMALFTLRPVFLK"
     gene            complement(1583..2119)
                     /locus_tag="PK742_08050"
     CDS             complement(1583..2119)
                     /locus_tag="PK742_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQU76584.1"
                     /translation="MKNWIYPAAAAIIVAASAFTLVEQRSWTIEDGYEIKFTSDDPTG
                     VFTAMAGDIVFDENDLENSYFKVKVDVSSINTGNGMQNKHAVSENWFDAEKYPHIGFA
                     SRDIEKTDKGFVAHGTLSIHGVEKEMDLPFKFEGASGKGVFKSKFQVNRTDFNLGDPG
                     NKASHVLQMEVTVPVSGK"
     gene            complement(2186..3454)
                     /locus_tag="PK742_08055"
     CDS             complement(2186..3454)
                     /locus_tag="PK742_08055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQU76585.1"
                     /translation="MAKAFSEFGFDDRVMEGIQTMGYETATPVQEQVIPLILEGRDIL
                     ASAQTGTGKTAAFLLPLMQRLLQEPHEEHHINALIIAPTRELAIQIDQNLQGISYFSS
                     ISSIAVYGGNDGGSFDQEKKALSQGADIVICTPGRMISHINLGYVKLQHLHYLILDEA
                     DRMLDMGFYPDLMRIIKNTPEKKQVLMFSATMPKLIAQMARQILKDPAEVNISISRPP
                     DKIRQRAYVIYEQQKAGLILHILKEHKDLKSILIFCSRKTSVKDLTRDLKREKLNADQ
                     IHSDLEQDQRADVLNRFASRRLQILVATDILSRGIDIDNIELVINYDVPHDGEDYVHR
                     IGRTARAENDGEAITLISPMEQRKFGSIERLIDKVVEKMPVPADLGEAPEYRPEQRSE
                     SGHRKPGGKGNNRNQRRRPRGDKNDKRGRS"
     gene            3649..5724
                     /locus_tag="PK742_08060"
     CDS             3649..5724
                     /locus_tag="PK742_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU76586.1"
                     /translation="MPAVLWGQTSISGIINTYTKVLAVDFCNNNVVVESAIGFIEGDQ
                     VLLLQMSGATLDQSNTATYGTITDVGLAGNYEILTIADVVFNVITFEETMERYYDAFD
                     GKVQLVTIPHYNDVDIDGEVTAAEYDGSKGGVVIFFADGTVTFNNEIDLTGQGFKGGD
                     DYSHLLCYGGTGNYDGYSCSEVEYCGSRKGEGIGKQLGTDSLGRGAPGNGGGGGNDSN
                     TGGGGGSNFGTGGQGGQRLNVGAGDCGGSYPGQGGKPLTYSNADNKIYMGGGGGSGDQ
                     NENDGSRGADGGGIVIIRADEIVGNGWAIKVNGRSVFEVAGRDAGGGGGGGGTVLLDV
                     ATYTGTINVQSIGGDGGDVDNNLDNVSCNGPGGGGSGGMLWVTNASLPAGITFTSTGG
                     QAGMTINPTAPAACNGSTNGATAGTAGGFLTGLVLPEPTTIFIPLTLEVTPDMAVICQ
                     GESVDLSAITDGTGVLTYQWNDPDNSTVTDLTVTPDNDYTYQFTVTDERGCQIVKSCI
                     VEVIDSVSAIAVPDATIVMGEFVTLSSNLLSDDFTYVWTPDYNISSTIYPVVTANPFS
                     TTEYCLTVTSNQAPCVTTSCVTIEVISDVVIPNAFSPNGDGVNDKFRVPELGDICNSI
                     TQFTIYDRWGEIVHDFQGGTGLGIGPTWEGRHYQTNIDLPIGTYIYFIRLDCDGGERI
                     FTGDVLLIR"
     gene            5777..6382
                     /locus_tag="PK742_08065"
     CDS             5777..6382
                     /locus_tag="PK742_08065"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HQU76587.1"
                     /translation="MRNWMMILVCMAIAVSTEAQVSSVQGSASEKNRAFKVDPFSPFT
                     GKLTLGYEQKLISNMNLDADFGIIGPNVFTNDRADAKGFFIKAGPRFYTHPDFFTDDM
                     KRYSDFQGFYFMPTVGYTQYAYTPLDLSFEGSDERRTQQAGSILLNIGRQWAVGDMVL
                     LDIGGGIGYGFASESARIKNFSHSGDNFIGSFTVSIGFLTR"
BASE COUNT         1669 a         1505 c         1750 g         1821 t
ORIGIN      
        1 ctgaataata ttccgctagc ttcgctggcg gccattttga tatttaccgg atacaagctg
       61 gccaagataa ccctgttcag ggaattctgg agtaagggta tggaccagtt tgttcccttt
      121 gtggtgacca ttgtggccat cctctttacg gacctcctga tcggcgtttg tattggcatt
      181 ggtatcagta tcatatttct attacgcacc aattttcaca gtgccatcat gatcgtgcat
      241 gatggagaca actatctgct tcgattcaga aagagtgtca gttttctgaa caaaccactg
      301 atcaaaaaag agctgagaaa gatcccttcc aacagccatc tgatcattga cacggccatg
      361 tctgatttca ttgacatgga tgtgatcgat acggtcaatg agttcctggt aagtgcccgc
      421 gacaggaaca tcacagtttc tatggacaag cacgggcaac gcaggtcgca ggcatttttt
      481 caaaccaatg ataactaaaa tattgtaata tgatgcattc aactgataag cttctgctgg
      541 agaacaaagc ctgggcgcag gaaatgatca cagatgatcc ggaatatttt tccagattgt
      601 cagatatcca gacccctgaa tttctctgga tcggctgtag tgacagtcgt gttccggcta
      661 accagatcac cggcacacaa cccggagaga tcttcgtgca caggaacata gccaatatgg
      721 tggtacatac agacctgaac ctgctcagtg tactggaata tgcagtagtt tatctgaagg
      781 tgaaacatgt gatcgtttgc ggccattatg gatgtggtgg tgttaaagca gcgatggaca
      841 gtcactctta cggcatgatc aacaagtggc ttagaaatat caaggatgtg tatcgctatc
      901 accgtgatga acttgccgga atcgaagacc aggagcaaag aaccaatcgt ttggtggagc
      961 tgaatgtcat ggagcaggta atgaatctgg ccaagaccag tatcattcag aaagcctggt
     1021 cagaagaaca aaggccagat ctgcatggct gggtgtacgg cctgcacgat gggttgatca
     1081 aaactgtata tgatatgcct gccggcacgc acctggaccc cttgtatgag tttgacaacc
     1141 tgaagcaatg aggtcaggat ctaaattaaa aagcccggtg caaaccgggc ttttttttat
     1201 ttcaaaaaaa cgggtcttag cgtaaatagt gccatagccg gaaagaagtg catgggcaaa
     1261 gccagtgccg gaaacacttc cggaaaatca atgtcaagtg gtaaagcata tgacagtggt
     1321 agcaccaggc tggcgatggt gatcacgctc agcaacaggt taaaggtgat atctctgcct
     1381 ttcttcatcc atctgttcag gatagcaaaa cctgccacag caaccatggt gcctatcata
     1441 ctggcagcca ggatggtcca caagttgatg acccggctga aatctgccat catggcgaag
     1501 aaatacaccc tgtcccagat gaaaccggcc agtgtactca tcagtccgct caacaaaccc
     1561 caaagaaata tctgcctgtt cattacttgc cggatactgg aacagttact tccatttgca
     1621 gcacatgaga agctttattt cccggatcac ccaggttaaa atcagttctg ttcacctgga
     1681 atttgctttt gaatacacct tttccacttg cgccttcaaa cttgaacggg aggtccattt
     1741 ctttttccac gccgtgaata gatagagtac cgtgtgctac aaagcctttg tcagtttttt
     1801 caatatctct gctggcaaat ccgatgtgcg gatatttttc tgcatcaaac cagttttcgc
     1861 tgacagcatg cttgttctgc atgccatttc ctgtgttaat ggaactcaca tctactttca
     1921 ctttgaagta ggagttctcc aggtcatttt catcgaacac gatgtcaccc gccattgcgg
     1981 tgaatacacc ggtgggatcg tcgctggtaa atttgatctc atagccatct tcgatggtcc
     2041 atgaacgttg ttccacaagg gtgaaagctg aggcggccac aatgatggcg gcggcagcag
     2101 ggtatatcca atttttcatc atacggtttt atgcaaatct caggccataa aaccgctggg
     2161 acattgaact agttcaagaa aattgtcagg atctgcccct tttgtcattt ttgtcgccgc
     2221 ggggacgtct tcgctggttg cggttgttgc cctttccgcc gggtttccgg tgtccgcttt
     2281 ctgagcgctg ttccggccgg tattcaggtg cttcacccag atctgccggc acgggcattt
     2341 tttccaccac tttatcgatc agtcgttcaa tggaaccgaa ttttcgctgc tccatcgggc
     2401 tgatgagggt tatagcctct ccgtcatttt ctgcccttgc ggttctgcct atacggtgca
     2461 catagtcttc gccatcgtgc ggtacatcat aattgatgac cagttcgatg ttgtcaatat
     2521 caatgcctct ggacagaata tcggtggcaa caagtatctg taatcgccgg ctggcaaatc
     2581 tgttcaggac atccgcccgc tgatcctgtt ccaggtcaga atggatctgg tctgcattta
     2641 gtttctcccg cttcaggtcg cgggtaaggt ctttcacgct ggtctttctg gagcagaata
     2701 tgaggatgct cttcaggtct ttgtgttctt tcagaatatg caaaatgagg ccggcctttt
     2761 gctgctcgta gatgacatat gcccgctgtc tgatcttatc cggtggtctt gaaatggaaa
     2821 tattgacctc tgccggatct ttcaggatct gcctggccat ctgggctata agttttggca
     2881 tggtagcaga gaacatgagt acctgttttt tttcaggggt gttcttgatg atgcgcatca
     2941 ggtcggggta gaaacccatg tccagcatgc ggtctgcttc atcgaggatg aggtaatgca
     3001 ggtgctggag tttcacatac cccaggttga tgtgagagat catcctgccc ggggtgcaaa
     3061 tgacaatatc agcaccctgg ctcagggcct tcttctcctg gtcaaatgaa ccgccgtcgt
     3121 ttccgccgta aactgcaata gaagatatgg aagagaaata ggatatgccc tgcaggttct
     3181 ggtctatctg tatggcaagt tctctggtag gagcaatgat cagtgcgttg atatggtgct
     3241 cttcatgagg ctcctgcaac aatcgttgca tcaggggcag cagaaatgct gctgttttgc
     3301 ctgtaccggt ctgggcgctg gccagtatgt cgcgaccctc cagaataagg gggattacct
     3361 gttcctgaac cggagtggcc gtttcatatc ccatggtctg gataccttcc atgaccctgt
     3421 catcaaaacc gaattcactg aatgctttag ccataatgca gaatgtgagc aaaggtagct
     3481 gaaaatatga gggacattga ccggtagcgg tatgtgctga aattgtgtga ctccggtgcc
     3541 ttgaattgca atgtcatgaa ctggtaaact tttctccatc tgttgcggaa agccgtatct
     3601 tcgtacccga tgttattgcg gcgtattttc tcctcagtgc tattttttat gcccgctgtt
     3661 ctgtgggggc aaaccagtat ctcaggtatc atcaatacct ataccaaggt actggctgtt
     3721 gatttctgca acaataatgt ggttgtggaa tctgccattg ggtttattga aggagatcag
     3781 gtactgctgc ttcaaatgag cggagccacc ctcgatcagt ccaatactgc tacctatggt
     3841 accataaccg atgtaggact ggcaggcaat tacgagatcc tgaccattgc agatgtggtc
     3901 ttcaatgtga tcacttttga agagaccatg gaacggtact atgatgcttt tgacggcaaa
     3961 gtacagcttg ttaccatacc tcattacaac gacgtggata ttgacggcga agtaaccgca
     4021 gcagagtatg acggatccaa aggtggggtg gtcattttct ttgcagatgg aaccgtgaca
     4081 ttcaataatg agatcgatct gaccgggcag ggcttcaagg gtggcgatga ttattctcac
     4141 ctcctgtgtt atggtggtac cggaaattac gacggatatt cctgttctga agtagaatac
     4201 tgcggcagca gaaagggtga aggtattggt aaacaacttg gaactgatag cctgggccgt
     4261 ggtgctcccg gaaatggtgg aggaggtgga aacgattcca ataccggagg tggaggaggc
     4321 agtaactttg gtacaggtgg ccagggcgga cagaggctga atgttggtgc cggcgattgc
     4381 ggaggaagct atcccgggca gggcggtaaa ccgcttacat acagcaatgc cgataacaag
     4441 atctatatgg gaggcggtgg tggcagcggc gaccaaaacg agaacgatgg cagccgtggt
     4501 gcagatggcg gcggaatagt cattattcgt gccgatgaaa ttgtaggaaa cggatgggct
     4561 atcaaagtaa acggacggtc tgtctttgag gttgcaggaa gagatgccgg tggtggcggt
     4621 ggtggcggag gaaccgttct gctggatgtt gctacctata ccggtacgat caatgtgcaa
     4681 tctattggcg gcgatggtgg tgatgtagat aacaatctgg ataatgtaag ttgcaatggc
     4741 ccgggtgggg gtggttcagg aggaatgctt tgggttacca atgcctcgct tccggctggt
     4801 atcactttca catctaccgg cgggcaggcc ggaatgacca tcaacccaac ggcacctgct
     4861 gcctgtaacg gctctaccaa cggtgccaca gccggcacag cgggtgggtt tctgaccgga
     4921 ctggtattgc ctgaacctac taccatcttt attcccctta ccctggaggt tacaccagat
     4981 atggctgtta tttgtcaggg tgagtcagtt gacctgtctg caattacaga cggtacaggt
     5041 gtgctgacct atcaatggaa tgacccggat aacagtactg ttacagattt aacagtaact
     5101 ccggataacg actataccta tcagtttacc gtaactgacg aaagaggatg ccagatcgtc
     5161 aaatcctgta tcgtagaagt gatcgactcc gtttcagcaa ttgcagttcc tgatgccacg
     5221 atcgtgatgg gtgaatttgt caccttgtct tccaatttgt taagtgatga ttttacttat
     5281 gtatggacgc cggactataa tatcagctct accatttatc cggtagttac ggctaatccg
     5341 ttttccacaa cagagtactg cctgacggta acaagcaatc aggcaccctg cgtaaccacc
     5401 agctgcgtta ccattgaggt gatcagcgat gtggtcattc ccaatgcttt ttcaccaaat
     5461 ggcgacgggg tgaatgataa gttcagggtt cctgagttgg gagatatttg caacagtatc
     5521 acgcaattta ccatctacga tcggtggggt gagatagttc atgattttca gggcggaacc
     5581 ggactgggta tcggtcctac ctgggaaggc aggcattatc agaccaatat cgatcttcct
     5641 atcggtacct acatctactt cattcgcctc gattgcgatg gaggagagcg gatctttaca
     5701 ggtgatgtac tcctgatcag ataagtgttt tttcagtttg gtacggtttt ggaattcctt
     5761 gggcaaaacg attgctatga ggaattggat gatgatcctg gtgtgtatgg caatagctgt
     5821 aagcactgaa gcgcaggtaa gctctgtaca gggcagcgca agtgaaaaga acagggcatt
     5881 taaggtggat cctttttcac cgttcacagg aaaactgacc ctgggctacg aacagaaact
     5941 gatcagtaac atgaatctgg atgcagattt cggcatcatt ggtccgaatg tgttcaccaa
     6001 cgaccgggct gatgcaaagg gattctttat caaggccggc ccccgcttct atacgcatcc
     6061 tgacttcttt acggatgata tgaagcgata cagtgacttt cagggtttct atttcatgcc
     6121 aacggtgggt tatacccagt atgcatacac ccccctggat ctcagtttcg agggtagtga
     6181 tgaacgacgc acccaacagg cgggaagtat tctgctgaac ataggcaggc agtgggccgt
     6241 tggcgacatg gtgctgcttg atataggagg tggcattggt tatggattcg cgtctgaaag
     6301 tgcacgcatc aagaacttca gccactcagg cgataatttc ataggtagtt ttaccgtcag
     6361 catcgggttt ctgaccaggt agaacaagcc gaaaaagcca aaaccccggt ctgatgaccg
     6421 ggggtttttg tatgtaatgt tggctctgtt attcttattc aaaagtgagg tcattcctgc
     6481 gaaagcagct tgcctgtccg gtaggcaggg gatctctcat tgatctattg accaactgtt
     6541 tgtttgagtg aacggtagct gagatgggag attcctgata gcctccttcg tcgccttcag
     6601 gaatgactta ttcttttgga ggggtttttg ggttatagat gatgtttatc cggaggaggg
     6661 gtcatccctg cgaaagcagg gatctcttac tgatccattg gccagctctt tacaggaatg
     6721 aacggaaaca gagataagag attcc
//
LOCUS       DAOMAX010000137         6712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1766_length_6712_cov_5.503230, whole genome shotgun sequence.
ACCESSION   DAOMAX010000137 DAOMAX010000000
VERSION     DAOMAX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_6712_cov_5.503230"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PK742_08070"
     CDS             <1..1367
                     /locus_tag="PK742_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76588.1"
                     /translation="LFEKVLNDPELSDMEAASFLQKNVGDSAFSMVKRRLKDDIIKVL
                     VWEQRHSKFRSKYFKSRFECRLLIAEAEILSSRGANLLAIEHLYKAKRLAEQFELTNE
                     LVIINEQLIPLVATREGLQTYSRIAQESLGQFKLIQDKFKAQDYLRQLTIPNLFQANK
                     EFNYKIQAEEASRELRKLSEETSSVEIRFWYLRSEVFYNHLRKDYTSAFQFAQDFLEL
                     VKTSPVVYSIDNMGGANMQLGMISIYRGEYESAALFANTAREYFTKGSVNFVNASDAL
                     FLSFFYRGLYDEAIVLLDEMKKNRNITRNHMLRSKWNFYDAQLHFRMGKYAESLSILQ
                     QQTDLFSDKSGWRIGIKILEMMCIVELGNDDWLDFRIETFRKLLSDLRKENIFRPKLI
                     HQLFRFYIKSGYDWKNAIRLSGKHLTLLQEGTGENSWDPLGYEMTRFDSWLSGRLNGS
                     SRSV"
     gene            complement(1364..6088)
                     /locus_tag="PK742_08075"
     CDS             complement(1364..6088)
                     /locus_tag="PK742_08075"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQU76589.1"
                     /translation="MSEATTGRKILRVTGKVLLWIPVVLSGLLPIFFLILQIPAVQTR
                     IRVAAVSWLEEKLDTTVKLDDIDINIFRHVVLDGLYIQDLEGDTLLYAEHAAIRIRSV
                     NPLSKDIRLRTLELKGCYAHVYRHPEDSSFNFQFIADAFASDKEKDTTSAPSALDLGI
                     KEIDFKQVHFQFDDSLSYQAYDTYLHSFRATFNKFSLQEEVLAVNQLEADGCTFQLSR
                     LLDLRPEEEDTIPSAYAYVHIRPGNWILEASHLQLKNCFFGYQNENHLLREDLMDFNH
                     LALRDINIDMEEIVYAGDTVRGYMNQVACTDRSGFRLQTLQSAFFFSGTKTILNGLLL
                     ETANSRITDNLQFSYASLLDFNRIFDAVEIRANLRNTVVSTDDLAYFSASLGSYHSLI
                     TANMPVSGTLSNLKASGAQLAVDQTLSFQGDLRIRGLPVIDETWFDVDLRPLQANMPG
                     LNEQLGGVLPSQITELGQVEFKGKYTGFIYDFVVYGQMESTIGSLNTDIRMQYDPDAG
                     NTHYSGSIATEELNAGLLAGLDDMLGTVSLVATVEGNSSPGKNDLVLSSQVSDIWFNG
                     YRYRDLQISGQLSNDGFDGRLNMNDPNARLSFLGSVHIADSTPVMNFESTIEHADLQL
                     LKIYPTPLTISAHTQVDITGSDINSFLGSAVLDEVHLSTGKYNWDQDRISLTSRQENG
                     VRNTRLESDLLNISVIGSYQVDQLPRQFTGMVNYYLHGTPFMTDSMVPQQFEYNISGH
                     DLHQATAIFYPDIEQLGILQLSGLFDSGQRHLTARWLLKNTQYKGIYTENVAGEISDR
                     EGHLSYFLRAAPIQVSEQLQLPVSSLEGALFQDSVDVNLKMGKDVDPERLNLYAGIKV
                     SEENLSLHVLPSEIFVSNERWDIQPNNSLIYDYENFYADQFTLTNLDKKVSITSSNKE
                     GMGAAFRVLIENFRLEDITKIAQYDDYALAGRIDANVTISDPFDSLTVLGFINIKEFI
                     LDAQPIGNINLTATRIFPNPHLNFNMNLQGANSMRAYGYYSIGETDSLHMVAEIAKIP
                     FKVAEPFTVGLMSDLRGDAYGTVRVEGPVNDLQTSGYVEMKNGGFYFDYLGIDMDFQF
                     QKVELFPDRIYLTPNKVFDESGNEGWVKGNIYHTAYDHFVFDSLVFNSGNFVLMNATV
                     KENPDFYGYTSGQVNAVIDGPLEDLQVTVDATPVKTPGKANVVYIPAYGSGNVSRHNF
                     IQFVDRSDSTGQAENNNKSLSVVSVSTFVQVTPDVEVQILLSSEGSDVIKGAGSGNLF
                     IDVNTLGKVEISGLLKITQGSYDFSFEGLATKKFIVKEGGTILFDQDPYQAKLGLTAV
                     YRLDKVQKLGLISDLPLSDPEIQEARKAIPVDVYINIGGTLEAPEISFDIILPDERSS
                     GGLSEFDQRLSEVKSDQNELNKQVFGLLMINNFLPKELNAGAALGAGVNSSISDFITS
                     QLSGYFSDWISEIIPNAEIDIGYQKIGSGDLGLSESDQTQFEAGLTQKLFGDALTVKI
                     GGTYNYEASSSNPNANLAGDFEVEYRITPDGRIRVKAFRESDFDAVASKNDVRTGLGL
                     FYTKDFDSFSELFESRRRQQTP"
     gene            6142..>6712
                     /gene="tsaD"
                     /locus_tag="PK742_08080"
     CDS             6142..>6712
                     /gene="tsaD"
                     /locus_tag="PK742_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQU76590.1"
                     /translation="MSLTILGIETSCDETAAAVVRDGKLLSSLVAGQEIHSRYGGVVP
                     ELASRAHQSAIIPVTDAALKEAGIRVADLDGVAYTRGPGLLGALMVGASFAKGLALAT
                     QIPLIPVHHMQAHILAHFIDAPVPAFPFICLTVSGGHTQIVRVNDYFRMEILGQTIDD
                     AAGEAYDKAAKMLGLPYPGGPQIDALAAQG"
BASE COUNT         1705 a         1615 c         1558 g         1834 t
ORIGIN      
        1 ggctatttga aaaggtgtta aacgatcctg aattaagtga catggaagcg gcctcatttt
       61 tacagaaaaa tgtaggtgat tcagctttct ctatggtcaa aagacgcctc aaggatgaca
      121 tcatcaaggt gttggtatgg gagcaaagac attccaaatt cagatctaaa tacttcaaat
      181 cacgctttga atgcaggttg cttattgcag aggcagagat cctgtcaagt cgcggtgcca
      241 acctcctggc catagagcac ctgtataagg ctaaacgcct cgcagagcag tttgaattga
      301 ccaatgagtt ggtgatcatc aatgagcagc ttataccatt ggtagccacc agagagggcc
      361 tacagaccta ttcaagaatt gcacaggaaa gtctcggcca gttcaaactg atccaggaca
      421 aattcaaggc ccaggattac cttcgccagc ttaccatccc taacctgttt caggccaaca
      481 aggaattcaa ttacaagatc caggctgaag aggcaagcag ggagctcaga aaactgagcg
      541 aagagaccag ttccgttgag atcaggttct ggtatctgcg tagcgaagta ttctacaatc
      601 acctcagaaa ggactataca tctgcatttc agtttgcaca ggattttctt gaactggtga
      661 agaccagtcc ggtggtttat tccatcgata atatgggcgg tgccaatatg cagctgggta
      721 tgatcagcat atacagaggc gaatatgaat ctgccgcttt gtttgccaat actgccagag
      781 agtatttcac gaagggatcg gtaaactttg tcaatgccag tgatgcactc tttctgtcat
      841 tcttttaccg cgggctttat gacgaggcca ttgttctgct ggatgagatg aagaaaaaca
      901 ggaatatcac caggaatcat atgctgcgtt ccaagtggaa cttctatgat gcccagcttc
      961 atttccgcat gggtaaatat gctgaatcac tatccatcct gcaacagcaa acagatttgt
     1021 tcagcgacaa atccgggtgg cgaataggca tcaagatcct ggaaatgatg tgcattgttg
     1081 aattgggaaa tgatgactgg cttgacttcc gtatcgaaac attcagaaaa ctgctgagtg
     1141 accttcgcaa ggagaatata tttcgcccaa agcttatcca ccagctgttt cgtttctata
     1201 tcaaaagtgg ttatgactgg aaaaatgcca ttcgattgtc tggtaaacac ctgacactgc
     1261 ttcaggaagg tacaggtgaa aatagctggg acccgctcgg ctatgaaatg accagatttg
     1321 attcctggct ttcaggccgt ttgaatggta gcagcaggtc tgtctaaggt gtctgctgcc
     1381 ggcgtctgct ttcaaacagt tctgaaaaag aatcaaagtc tttagtataa aataaaccca
     1441 gacctgtacg tacatcgttc ttggaagcca ccgcatcaaa atctgattct ctgaacgctt
     1501 tgacccgtat ccttccgtca ggtgtgatgc ggtactctac ctcaaaatca cctgccaggt
     1561 tggcatttgg attactgctg ctggcttcat agttgtaagt accacctatc ttgacggtta
     1621 gtgcatcgcc aaaaagtttt tgtgtaagtc cggcttcaaa ctgcgtctga tcagactcgc
     1681 tgaggcccag atctccggag ccgatcttct ggtacccgat gtcgatctct gcattgggta
     1741 tgatctctga gatccagtct gagaagtatc ctgacagctg actcgtaatg aagtcagaaa
     1801 tgctgctgtt gacccccgct ccaagtgcgg cacctgcatt cagttcttta ggcaggaaat
     1861 tattgatcat gagcaaaccg aaaacctgtt tgttcaattc attctgatct gatttgacct
     1921 cagataatcg ctggtcaaac tcagacaaac ctccgctgct tctttcatcc ggcaaaataa
     1981 tgtcaaaact gatctccggc gcttccagag tgccaccaat attgatgtac acgtcaaccg
     2041 gaatggcttt cctggcttcc tgtatctccg gatctgacag cggcagatca ctgatgagcc
     2101 ccagcttctg aactttatcc aggcgatata ctgcggttag tcccagtttg gcctgatacg
     2161 gatcctgatc aaagagtatc gtgccacctt ccttgactat gaacttcttg gtagccagac
     2221 cctcaaatga aaaatcatag ctgccctgtg tgatctttag caagccgctg atctccactt
     2281 ttccaagcgt gttcacatct atgaacaggt tgccggatcc tgctcccttg atcacatctg
     2341 aaccttcact gctcagcagg atctgcacct ctacatctgg tgtaacctgt acaaaggtgc
     2401 ttacactcac cacactgagt gatttgttgt tgttctctgc ctggccggtg ctgtctgatc
     2461 tgtcaacgaa ttgaatgaaa ttgtgcctgc tcacatttcc tgaaccatag gccggtatgt
     2521 aaaccacatt cgccttgcct ggggtcttga caggtgtagc atctacggtt acctgcaggt
     2581 cttcgagcgg cccgtctatg acagcattta cctgtccgga ggtatagcca tagaagtcag
     2641 gattctcttt gacagtggca ttcatcagca caaaattacc tgagttgaat accagactgt
     2701 caaatacaaa atgatcatat gcagtatggt agatattgcc ctttacccat ccctcattac
     2761 cggactcatc aaagaccttg ttgggtgtca gatagattct gtcaggaaat aattcaacct
     2821 tctggaactg aaagtccata tcaatgccca ggtagtcaaa gtagaagcca ccatttttca
     2881 tctccacata ccctgaagtt tgcaggtcgt ttaccggccc ttccacacga acggttccat
     2941 aggcatcgcc ccggaggtct gacatcaggc ctacggtgaa cggctctgct accttgaacg
     3001 ggatcttggc gatctctgcc accatatgga gactgtcggt ttcacctatg ctgtaatacc
     3061 cgtatgcccg catggagttg gccccctgca ggttcatatt aaaattcaga tgaggattcg
     3121 gaaaaatgcg ggttgcggtc aggttgatgt tgcctattgg ctgtgcatcc agaatgaatt
     3181 ccttgatgtt gatgaaaccc agtacggtca gactgtcaaa tggatcgctg atggttacgt
     3241 tggcgtctat tctgccggcc agtgcatagt catcatactg ggcgatcttg gtgatgtctt
     3301 ccagtctgaa attttcgatc agcacacgaa aggcggcccc catgccttct ttattgctgc
     3361 tggttatact cacctttttg tccaggttgg tcagggtgaa ctgatcggca taaaaatttt
     3421 cgtaatcata gatcagtgag ttgttgggtt gtatgtccca gcgttcattg ctcacaaaga
     3481 tctccgatgg aagtacgtgt agtgaaagat tctcttccga tactttgatg ccggcataca
     3541 ggttgagtct ttccggatcc acgtcctttc ccattttcag gttgacatca acagaatcct
     3601 ggaacaaggc tccttccaga gaagaaacag gtagctgcaa ttgctctgaa acctgtatcg
     3661 gtgcagctcg caggaagtaa ctcagatgac cttcacggtc tgaaatttca cctgcaacat
     3721 tttctgtata gatgccctta tactgcgtgt tttttagcaa ccacctggcg gtcaggtgtc
     3781 tttgtccgct gtcaaaaaga ccgctaagct gcaatattcc caattgttct atatccgggt
     3841 agaaaattgc tgttgcctga tgcagatcat gtccgctgat gttgtattcg aattgttgcg
     3901 gtaccatact gtcagtcatg aacggggttc catgaagata gtagttcacc ataccggtaa
     3961 attgccgcgg cagctgatct acctggtaag aaccgatcac tgaaatgttc agcagatcgc
     4021 tctccagccg tgtatttctt acgccatttt cctgcctgga agtcaggctg atccgatcct
     4081 gatcccagtt gtacttgcct gtagataaat gcacctcatc cagtacagca ctgcccagaa
     4141 aggagttgat atcgcttccc gttatatcaa cctgtgtatg tgcactgatg gtaagcggag
     4201 tgggatagat cttcagcaac tgcaggtctg catgttctat ggttgactca aagttcatga
     4261 ccggtgtact gtcggcaata tgaacagatc ccagaaagga aagacgtgcg ttgggatcgt
     4321 tcatattcag tctgccatca aagccgtcat tacttagctg acctgaaatt tgcaggtcgc
     4381 ggtatcggta accgttgaac cagatatctg atacctgtga actcaatacc agatcattct
     4441 ttcctggcga actgtttccc tcaaccgttg caaccaggct gactgttccg agcatgtcat
     4501 ccaggcctgc cagcaatccg gcattcagct cttctgtagc gatgcttcct gagtaatgcg
     4561 tatttccggc atccggatcg tattgcatcc tgatgtctgt attcaaactg cctatggtac
     4621 tttccatctg tccatagact acaaaatcat atataaaacc agtatattta cctttgaatt
     4681 ccacctgacc cagttccgtg atctggctgg gtagtactcc tcccagttgt tcattgagac
     4741 cgggcatgtt ggcctgcagc gggcgcagat ccacgtcgaa ccaggtttcg tcaatgacgg
     4801 gcagaccacg aatgcgcaga tcaccctgaa agctcagtgt ttgatcaact gccagctgtg
     4861 caccactggc cttcaggttt gacaaggttc ctgatacagg catattggcg gttatcaggc
     4921 tgtggtaaga acccagtgaa gcactgaagt aagccagatc atctgtactg acaacagtat
     4981 ttctgagatt ggcacggatc tcaacagcgt caaatatcct gttgaaatca agcagagagg
     5041 cataactgaa ttgcagatta tctgtgatcc tgctgttggc tgtttccaac agcaatccgt
     5101 tgaggatggt ctttgttcca ctgaagaaaa aggctgactg caaggtttgt aaacgaaatc
     5161 cgctgcgatc tgtacaagcc acctgattca tgtatcctct cactgtatca ccggcatata
     5221 cgatctcttc catatcgata ttgatatctc gcaatgccag atggttaaag tccatcaggt
     5281 cttcacgcaa cagatggttt tcattctgat acccgaaaaa gcaatttttc aactgaaggt
     5341 gagatgcctc caggatccag ttgcctggtc ggatgtgaac ataggcatat gctgaaggaa
     5401 tggtgtcttc ttcttccggc cgaaggtcca gcaatctgga taactgaaag gtacatccgt
     5461 ctgcttccag ctggtttact gccagtacct cctcctgcag gctaaactta ttgaaagtag
     5521 ccctgaacga atgcaggtag gtgtcataag cctgatagga cagactgtcg tcaaactgaa
     5581 agtgtacctg tttgaaatcg atctctttga tcccgagatc cagagccgaa ggagcactgg
     5641 tggtatcttt ctccttgtct gatgcaaacg catctgctat gaattgaaaa ttgaacgaac
     5701 tgtcttccgg gtgcctgtac acatgtgcat agcacccttt cagctccagg gtgcgcaaac
     5761 gaatatcttt cgacaatgga tttactgatc tgatacgaat ggccgcatgt tcagcataca
     5821 ataaggtgtc tccttccaga tcctgaatgt acagtccgtc cagaactaca tggcggaaaa
     5881 tattgatatc tatgtcgtcc agcttcaccg ttgtgtccag cttttcttcc agccatgaaa
     5941 ctgcagctac cctgatccgg gtctgtacgg caggtatttg taaaatgaga aaaaagatgg
     6001 gcagcaagcc tgaaaggaca accggtatcc acaagagaac ctttccagtc accctcagta
     6061 tcttcctgcc ggttgtagct tcggacatac gatgtacact gctagtgcag taatttagcg
     6121 aaattattaa atgattatta catgtcgctg accattttgg gtattgaaac atcctgcgat
     6181 gaaactgctg cagctgtggt cagagacgga aagttgctgt ccagcctggt agccggtcag
     6241 gagatccact cccggtatgg tggtgtggta cctgaactgg catcgcgagc acaccaatcg
     6301 gccatcatcc cggtaacaga tgcggcactg aaagaagccg gtatcagagt tgccgatctg
     6361 gatggggtgg cttatacccg tggtccggga ttgctcgggg ctctcatggt tggcgcatct
     6421 tttgccaaag gcctggccct tgcaacgcag attccgctta ttcctgtaca tcatatgcag
     6481 gcacatattc ttgctcattt tattgatgca ccggtaccgg catttccatt catttgtctc
     6541 actgtttcag gcggacatac ccagatcgtt cgggtaaatg actatttccg gatggagatt
     6601 ctgggtcaga cgatagatga tgcagccggc gaagcatatg ataaggcagc caagatgctg
     6661 gggctccctt atcccggcgg accgcagatt gatgctctgg cagcacaggg ag
//
LOCUS       DAOMAX010000138         6705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1769_length_6705_cov_4.369173, whole genome shotgun sequence.
ACCESSION   DAOMAX010000138 DAOMAX010000000
VERSION     DAOMAX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_6705_cov_4.369173"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..838
                     /locus_tag="PK742_08085"
     CDS             272..838
                     /locus_tag="PK742_08085"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HQU76591.1"
                     /translation="MTLRLILYAAAYIMLVLVMFLLPYYSASGYSVVKHTTSQLGAQH
                     TPNAWIMNLTFILMGFSSILAGWDYFQGYWFHRICLLVFGISLILVAYFHHAPIDALT
                     PFNKHQDDLHSLFATTTGISFILLAICTAFLQTGRMHMILALSIAILACLFSILIFRF
                     PQLAGIWQRSLFVISFGWLLYEWSRKDL"
     gene            complement(899..2092)
                     /gene="porV"
                     /locus_tag="PK742_08090"
     CDS             complement(899..2092)
                     /gene="porV"
                     /locus_tag="PK742_08090"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HQU76592.1"
                     /translation="MKNALKCLPVVALLILAGNLQAQVTVPTSADGEDYLNTVTTAVP
                     FLRIAPDARGGSMGDVGIATSPDGASIFWNNAKFAFHEENSGLAITYTPWLSNLVNDI
                     FLAYLNGFWRTDELSVVSGSLRYFSLGNITFTDQNGQVLQDFRPNEFALDFGYARKLS
                     DNFSTGLVLKYIYSNLASGQFVNGIPIKPANGVAADISVFYTGEVEMGSKDAFINAGA
                     NIANLGNKVTYTSSTERDFIPTNLGVGASIGVNFDEYNKLAWSIDFNKLLVPTPDTTD
                     IDPANGIPDYKELSPVSGVFSSFSDAPNGFSEELNEITIGTGLEYWYNNQFAVRGGFF
                     FEDPTKGGRQFFTLGLGLKYNVFGLDFSYLIPSSNQQNPLDNTLRFTLSFDFEALASD
                     AEPAE"
     gene            complement(2131..6000)
                     /gene="porU"
                     /locus_tag="PK742_08095"
     CDS             complement(2131..6000)
                     /gene="porU"
                     /locus_tag="PK742_08095"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HQU76593.1"
                     /translation="MSWMHRTSVSRILGFAMLTLFHWSAVYAAGEEYRISLSLNWHTS
                     PLSVTAEDGFSARYYAGFEGASYEALGASIVPVYSANLPVSVKGTVTVTLQEAVWESI
                     RWPGRAIPELAEAPAFRTFIGLEKGRPMLHCSFVPLRKNPSTGQLEKLVSGRLELSIL
                     PSATRMDETEHDYADHSRLQTGTWVRISVTSNGIYKIDRALLESAGISTATLNPATLG
                     VFGYGGGMLPEANDDHNPDDVVELPLYKVGLDDGSFDDGDYVLFYGESPNQWTYNETT
                     DAYRQYNHLYANRTSYFISPDRGTNLTLSTRNNSAESPTYTTSSYDYLQAWNNDVVNL
                     ISSGRQFFDENFTNFSNSRNYSATIPNIIAGETGRIRYFVAAKSASGSSQFTLSGNGI
                     NSTTTLPAQPESTENDYASHLQFESVFTQTSSSAVNINVNYSGPSGGNGWLDNIELVF
                     RCNLNAGSGQFRFRDSRAVGETAQYTITGAGSNLLWDVTDPRHPLIQEYSGSGTISFT
                     VEADSLLDFILFNTADAFPSDQIQVDGTVANQDIHNVSLHPDFVIVSDAAYMAEARRL
                     AGWHHDVNDLDTLVVSVSQVYNEFSSGTPDITAIRNMMRMFYDRAAGVEDLQPKYLLF
                     MGDASFDYKSIQTSSEDNTNRVPTYQSYESIHQVQTFPTDDYYVCLDPGEGADMSESV
                     NLLDLACGRFPVNSVTEANEMVDKMIHYKSTTTFGNWRNTVCFVADDEDGNTHIDDAN
                     DIADWVTTNHPLYNLNKIYLDAYQQIPGAGGERYPDVNRDLNNQIFSGALILNWTGHG
                     NEQNWAQERILGVDDINSWTNYDKLPLFITATCSFSRFDNPERTSAGELILLNAGGGG
                     IGLVTTVRIVYSYQNYVLNSNFFYKIFEPVGGVMPTIGEALMAGKNTASDGFSAVNNR
                     KFLLLGDPALTLNYAKYDVVTTKVNDTPISDGTDTLKALARVTIEGEIRSGGSLLSGF
                     NGVVYPTVYDKPVSVTTLENDPEGSNPYTFSQQKSLIYKGKASVVNGAFSFEFIVPKD
                     ISYTFGNGKLSYYADNGLEDAAGYEFNVVVGGAADGALEDAEGPDVEVYMNDESFVFG
                     GMTDENPVLYLKLLDESGINTVGNGIGHDITATVNESSEEYRLNDYYEADLDSYQSGT
                     VRYPLYDLPAGRHAVNVKAWDVYNNSGDGYTEFVVAESAELALDHVLNYPNPFTTYTE
                     FWFEHNRPGDLLDVKVEIFTVSGKLVKTIMTQMATDGYRVDGIGWDGLDNFGDPIGKG
                     VYVYKLTVQAASDRSRVSAFEKLVILR"
     gene            6052..>6705
                     /locus_tag="PK742_08100"
     CDS             6052..>6705
                     /locus_tag="PK742_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU76594.1"
                     /translation="MLIACCRKAVSSFEPKVLSLLWFLALGHFSATFVQAQDLRFTQW
                     HASDMVLNPAFAGAGAGGRFTANFRSQWPDMPQTYLSYRICYDQPISDVNSGFGIYML
                     QDDLGDQVLKNLNLGFQYMYQAKVSSSAAFNFGAQIGMVQRKLNWNQLQFYDQINPVY
                     GFNNAQGIPNPTNEPQPADLSTTVLDLGLGAAFVTEKAYTGISFLHVNQPPNTFYDYE
                     "
BASE COUNT         1594 a         1635 c         1676 g         1800 t
ORIGIN      
        1 gccgcatgat attgatggca gcgtcatgcc cgtcgaacag gctggccgcg gtcacgatgc
       61 gtaccttatg ggtcgggttg taaggggtaa cttcctgcat acctgaatat tagaacaaag
      121 attggtatgt aaagttacga agagccgctg ttaactgcag ttttggtatg aaggtgctca
      181 taaacagttg ttgagaatgt catgagataa gtcatattaa ccgggcattt gtgcaaccat
      241 aacgggcaga tggtatttgt aatttgcatt tatgacactg cgattgatac tttacgcagc
      301 agcatatata atgttggtgt tggtcatgtt cctgctgcct tattattctg catcaggata
      361 ctctgtggtg aaacatacta ccagtcagct cggggctcag cacacaccca atgcatggat
      421 catgaatctg acatttatac ttatgggctt ctcttcgatc ctggcaggct gggattattt
      481 tcagggttat tggtttcatc gaatttgttt gctggtgttt ggtatttcat tgatcctggt
      541 ggcatacttt catcacgcac ctatagatgc tttgacaccg ttcaataaac atcaggatga
      601 cctgcacagt ctgttcgcca caaccacagg tatcagtttc attttactgg caatatgcac
      661 tgcttttctt cagacaggaa gaatgcatat gatccttgcg ttgtccattg ccattctggc
      721 ttgtttattc tctatactga tatttcgttt tccgcaactg gcaggtatct ggcaacgatc
      781 gctttttgtt atttcttttg gttggctgct gtatgaatgg agcagaaaag acttatgagt
      841 acaagcaaaa aaaaggcagc caacaggctg cctttccaaa agaatagtat gatctttttt
      901 attctgccgg ctctgcatcg ctggccagtg cttcaaagtc gaagctcagg gtaaatctca
      961 gggtgttgtc aagcgggttc tgctggttgc tgctgggaat caggtagctg aagtccagtc
     1021 cgaacacgtt gtatttcaaa ccaagtccca gggtaaagaa ctggcggcca cctttggtgg
     1081 ggtcttcaaa gaagaaaccg ccacgaacag cgaactggtt gttgtaccag tattccagtc
     1141 cggtgccgat ggtgatctca ttgagttctt cactgaaacc attaggtgca tcgctgaagg
     1201 agctgaatac accacttaca gggctgagtt ccttgtaatc aggtattccg ttggcagggt
     1261 caatatctgt ggtgtctggt gtaggtacca gaagcttatt gaagtctatg ctccatgcca
     1321 gtttgttgta ctcatcaaag ttcacaccga tgcttgcacc aacacccaga ttggttggaa
     1381 tgaaatcacg ctctgtgctg gaagtatagg tgaccttgtt gcccaggttg gcaatgttgg
     1441 ctccggcatt gatgaaagcg tctttactgc ccatttccac ttctcctgta taaaacacag
     1501 agatatctgc agctacaccg tttgccggtt tgataggaat accgttcacg aactgtccgg
     1561 atgccagatt ggaataaatg tatttcagta ccagtccggt agagaaatta tcgctcagct
     1621 ttctggcata gccgaagtca agggcaaatt cattcggacg aaaatcctgc agcacttgtc
     1681 cgttctggtc ggtgaaggtg atattaccca gagaaaaata tcgcagggag ccacttacta
     1741 ccgataattc atcagtgcgc cagaatccgt tcagataggc caggaaaata tcgttcacca
     1801 ggttgctcag ccagggtgta taggtaatgg ccagccccga gttctcttcg tgaaaagcga
     1861 acttggcatt gttccagaaa atggatgcac catcgggtga agtggcaata ccaacgtcgc
     1921 ccatactgcc acctctggcg tcaggggcga tgcgcagaaa aggtacggca gtggtcacgg
     1981 tgttgagata atcctcaccg tcagcacttg ttggtacggt cacttgagcc tgcaggttgc
     2041 cggccaggat cagtaaagcc acaacgggca agcatttcag agcattcttc atgaaagata
     2101 attggattgc aaagttaata tttaatggtt ttaccttaaa atgaccagtt tttcaaaggc
     2161 tgacacccgc gaacgatcgc tggcagcctg caccgtgagc ttatacacat aaactccttt
     2221 tccgatagga tcgccgaaat tgtccagccc gtcccagcct attccgtcca cccggtagcc
     2281 atcagtggcc atctgggtca tgatggtctt gaccagtttt ccgctcacgg tgaagatctc
     2341 cacttttaca tccaggaggt cgccgggacg attgtgttca aaccagaact ctgtataagt
     2401 ggtgaaagga ttcgggtaat tgagcacatg gtccaacgcc agttcagcag attctgctac
     2461 cacaaattcc gtatagccat cgccggaatt gttatataca tcccaggcct tcacattcac
     2521 cgcatggcgt cctgccggaa gatcatataa cggatagcga accgtgccgc tctgatagga
     2581 atcgagatcg gcctcataat aatcgttcaa acgatattct tctgaagatt cattcaccgt
     2641 tgccgttatg tcatgcccga taccattacc cacggtattg atgccactct catccagcag
     2701 tttcaggtag agcaccggat tctcatcggt catcccgcca aatacaaagc tttcgtcgtt
     2761 catatacact tccacatcgg gtccttcggc atcttccagg gcaccatcgg cggccccgcc
     2821 cactaccaca ttgaattcgt atcctgcggc atcttccagg ccattgtctg catagtagct
     2881 gagcttacca tttccaaagg tgtaactgat gtctttcggt actatgaact caaaactgaa
     2941 ggcaccgttc accacactgg cctttccttt atagatcaga cttttctgct gagaaaaggt
     3001 ataggggttg ctgccttccg gatcattttc cagcgtagtg actgaaacag gcttgtcata
     3061 gaccgtagga tagaccactc cgttgaatcc actgagtaag ctgccgccgc tgcgtatctc
     3121 accttcaatg gttaccctgg caagtgcctt gagggtgtct gttccatctg aaatgggggt
     3181 atcgttgacc ttggtggtca ccacatcata tttggcataa ttgagggtca gcgcaggatc
     3241 gcccagtaac aggaatttcc ggttgttcac agcgctgaac ccatcgctgg cggtgttctt
     3301 tcctgccatc agtgcttcgc caatagtagg catgacgcca cctaccggtt cgaagatctt
     3361 atagaagaag ttgctgttga gtacgtagtt ctggtaagaa tacacgatgc gcaccgtggt
     3421 gaccagccct atacctccgc cacctgcgtt gagcagaata agttcgcctg cagaggtgcg
     3481 ttcaggatta tcaaacctac tgaaagagca ggttgctgtg atgaacaggg gcagcttgtc
     3541 gtagttggtc caggagttga tatcatccac acccagtatc ctttcctggg cccagttctg
     3601 ttcgtttcca tgaccggtcc agttcaatat cagtgcaccg gaaaagatct ggttgttgag
     3661 gtcgcggttc acatccggat agcgttcacc ccctgcaccc ggaatctgct ggtaagcatc
     3721 cagatagatc ttgttgaggt tgtagagcgg atgattggtt gttacccagt cggctatgtc
     3781 attggcgtcg tcaatatggg tgttgccatc ttcatcatcg gccacaaaac agacggtgtt
     3841 gcgccagttg ccgaatgtgg tggtcgattt gtaatggatc atcttatcca ccatctcatt
     3901 ggcttcggtt acgctgttga ccggaaacct gccgcaggca agatccagca ggttgacaga
     3961 ttcgctcata tctgcacctt cgcccggatc cagacaaaca taataatcgt cagtagggaa
     4021 ggtctgtacc tggtgaatac tttcatagct ctgataggtg ggaacgcggt tggtattatc
     4081 ttctgatgag gtctggatgc tcttgtaatc gaaactggca tcgcccatga acagcagata
     4141 ttttggttgc agatcttcta ctccggcagc ccggtcatag aacatgcgca tcatatttct
     4201 gatagcggtg atatcaggtg tgccggaact gaattcatta tacacctggc tgacgctcac
     4261 taccagtgta tcgaggtcat tgacatcgtg gtgccagcct gccagtctgc gggcttcggc
     4321 catataggct gcatctgata cgatcacaaa gtcgggatgc agcgaaacat tgtgaatgtc
     4381 ctgattggca acagtaccat ccacctggat ctgatcagag gggaaggcat ctgctgtatt
     4441 gaacaggata aaatcaagca ggctgtctgc ttctactgta aagctgatgg ttccgctgcc
     4501 gctgtattcc tggatgaggg gatgtctcgg atcggtcaca tcccacagca gattactgcc
     4561 ggcacctgtg atggtgtact gcgctgtttc gcccactgcc cggctgtcgc ggaaacggaa
     4621 ctgtccgctg cccgcattaa ggttgcacct gaacaccagt tcaatattgt ccagccatcc
     4681 gtttccgcca gatggtccgc tgtaattcac attgatgttt acagcagatg atgatgtttg
     4741 tgtgaacacc gactcaaact gcaaatgcga agcataatcg ttctccgtgc tttccggttg
     4801 tgccgggagg gtagtggtac tgttgatgcc atttccgctc agggtgaact gagatgaacc
     4861 ggaggcgctt ttggctgcca cgaagtagcg tatgcggcct gtttcgcctg cgatgatatt
     4921 ggggatggta gccgaataat tcctgctgtt gctgaaattg gtgaaattct cgtcaaagaa
     4981 ctgccggcct gaactgatga ggttgaccac atcattgttc catgcctgca ggtagtcata
     5041 agaactggta gtgtaggtgg gcgattcagc cgaattgttt cgggtgctca gggtcagatt
     5101 cgtacctctg tccgggctga tgaaatagct ggtgcggttg gcatacaggt ggttgtattg
     5161 tctgtatgca tcggtggttt cgttgtaggt ccactggttg ggcgactcac cataaaagag
     5221 tacatagtca ccgtcgtcga acgaaccgtc atccagacct accttatata atggaagctc
     5281 caccacatcg tccggattat ggtcatcgtt tgcttcgggc agcatcccac ccccataacc
     5341 gaacactccc agagtagcag gattcagcgt agctgtagag atgcctgcac tctccagcag
     5401 ggcacggtct attttataga taccattgga agtgacactg atgcgcaccc aggtgccggt
     5461 ttgcagcctg gaatgatctg catagtcgtg ttcggtttca tccattctag tggccgatgg
     5521 caggatggac aattccagcc tcccgcttac cagtttttcc agttgtccgg tggatggatt
     5581 tttccgcagc ggaacgaaac tgcagtgcag catcgggcgg cctttttcca ggccgatgaa
     5641 ggtgcggaat gccggagctt ccgccagttc agggatggct ctgcccggcc agcggatgct
     5701 ttcccatacg gcttcctgta aagttactgt cacagttcct ttaacagata ccggcaggtt
     5761 ggcactgtaa accggtacaa tggacgctcc cagagcctcg taggaagctc cttcaaaacc
     5821 tgcatagtaa cgggcggaga agccgtcttc cgctgttaca gagagaggtg atgtatgcca
     5881 gttgagtgac aggctaatgc ggtattcttc gccggcggca tatacagccg accagtgaaa
     5941 aagcgttaac attgcgaaac ccaggatccg ggaaacggag gtccgatgca tccaactcat
     6001 actacagtca aggttcaaat aataaaggcc accccaaaac ggcatctgca tatgcttatt
     6061 gcctgttgca ggaaggctgt cagctccttt gaaccaaagg tactaagcct gctttggttt
     6121 ctggcgctcg gtcatttttc tgccaccttt gtacaggcgc aggatctgcg ctttacccaa
     6181 tggcatgcca gcgatatggt gctcaatcct gcatttgccg gtgccggtgc cggaggccgc
     6241 tttacagcca atttcagaag ccagtggcct gatatgccac aaacctatct gagttacagg
     6301 atctgctatg atcagcctat cagtgatgtg aacagcggtt ttggtattta tatgctgcag
     6361 gatgacctgg gagatcaggt tttgaagaat ctgaacctgg gttttcagta tatgtaccag
     6421 gctaaggtaa gctcttctgc tgcattcaat ttcggtgcac agatcggtat ggtacagcgc
     6481 aaactgaact ggaaccaact gcagttttat gatcagatca atccggtgta cggattcaat
     6541 aatgcccagg gtattccaaa ccccaccaac gaacctcagc ccgccgacct cagcaccacg
     6601 gtactggacc ttggattagg cgctgctttt gtcacagaaa aggcctacac aggcatttct
     6661 tttctgcatg tgaaccagcc tcccaatacc ttctatgatt atgaa
//
LOCUS       DAOMAX010000139         6703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1770_length_6703_cov_4.221119, whole genome shotgun sequence.
ACCESSION   DAOMAX010000139 DAOMAX010000000
VERSION     DAOMAX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1770_length_6703_cov_4.221119"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK742_08105"
     CDS             complement(<1..252)
                     /locus_tag="PK742_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76595.1"
                     /translation="MTTRPEPILIPLNGAFWLHLAITLFAWVGPFLIDWWLMCAIYAI
                     VLLQFAVLNKCVVNAGHDLEEGEGITFYAFLFESIGIYFP"
     gene            337..2133
                     /locus_tag="PK742_08110"
     CDS             337..2133
                     /locus_tag="PK742_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76596.1"
                     /translation="MKHTLLTTVFFIGTIALQAQTCPGCSIDYSYTSPGLYPEILPPA
                     TAGEYYEVDLTFLLQEDTTVEVLGSDVTFAFLNYHILEPIGLPYGIHVTSNLGDFPVD
                     YDPADGLFGCARVCGTPLVAGSYTITVPLIATLEDPAGDNPAEYELSLEVLPPSATDG
                     GIVASTTFGCSPLTVDLSTGHPSDGAGGFSYTWDFGDGTYSILEDPVSVTLTADGTEP
                     VEYVVTHTVTIDTIGYNLDYVTVLSTGCDDCAFFGCTGLFAEENPDIYLYFPELGITT
                     DYYLDTNPPVTFSLNTALDPAATYNMQVKDDDDGGFGADDNCGNLMFSGDDVGVFTYD
                     IGSSDVEISISHPIIFYTYTDTIVVYPVPSIPELTISGETELCEGDSVLLSTTPDPTV
                     TYQWLQDGDPIPSAFDADFYAMETGTYALQATATGGCTAISESVDVVVYELPFPPFVS
                     IIGNTLQTSSDYPIQWYYSGDPIPGANDSIYEPAIEGIYQAAAINGPCISFSSEIDFV
                     FQSVLDLDPFQLTIAPNPVDDMLFLSGNTAVAGTMTIQIINATGQVIRSKTLSVGSVF
                     SLQLPMDEIPSGYYRLIMRSAEQQGSYPFLRQ"
     gene            complement(2127..3065)
                     /locus_tag="PK742_08115"
     CDS             complement(2127..3065)
                     /locus_tag="PK742_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQU76597.1"
                     /translation="MRGRFVFPLVLMLLSQVLAAQLPMPELSAGTLIRIDSFASSYVD
                     ARPVDIWLPPGYETTQQYPVLYMQDGQMLFDARTTWNKQEWGMDEVIPALIDSGQIQA
                     FILVAVWNNAPVRYLEYTSMKPFVYFSDSDMAILRDEPATGRLFTGDNLKGDLYLRFL
                     VSELKPYIDSHYPTLSGREDTFIMGSSFGGLISMFAICEYPEVFGGAACLSTHWTGLY
                     RQENNPFPFAMLAYFKDHLPDPSTHKLYFDHGTTTLDSLYAPYQQSADQIMREGGYTA
                     ANSLSLVFEGAPHTEEAWHDRVHIPITFLFGKGDDH"
     gene            3128..4105
                     /gene="moaA"
                     /locus_tag="PK742_08120"
     CDS             3128..4105
                     /gene="moaA"
                     /locus_tag="PK742_08120"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQU76598.1"
                     /translation="MLSDTFGRRHDYLRISLTDNCNFRCRYCMPEEEMQFHPDEQLMQ
                     PDEIEALAKIFVQLGTRKIRLTGGEPLVRKDAGNIIERLAALPVQLTLTTNGFLADRY
                     IDLFHASNIRSLNISIDSLSPEKFLAITRRDKFDTVWKNILTMVEQDFHVKLNCVVME
                     GVNDDELLDFVALTKDLPVHVRFIEFMPFTGNHWNNKAVFTWQQMLERISGKFLFKKL
                     SDEPNDTAKKYQVPGHAGTFAVISTMSAPFCSTCNRMRLTADGKMKNCLFSAGETDLL
                     TALRKGDDVEAMIRKNLDEKQEMMGGQFTDYSVTNPDEIQNRTMISIGG"
     gene            4122..5309
                     /locus_tag="PK742_08125"
     CDS             4122..5309
                     /locus_tag="PK742_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQU76599.1"
                     /translation="MISVDEAIQLVIGHTPSPRTITLPLQACAGHVLAQDILSPGPFP
                     SFDQSAMDGYAFCFADWQQNPLPVDGETAAGQFPEPLIQGTTRRIFTGAAIPPGADTV
                     VMQEHVQTHDGVIHIENSTLIAGANVRKQGSQIQAGECALPSGHTLQAAGIGFLASMG
                     FDRATVFEKPVIHIVVTGSELLPPGAPAEAGKVYESNSVMLLAALRSIGMEAQAVHHV
                     PDDPQATLALLDRLSTECDYILATGGVSVGDYDFVGQMKHHPAFQSIFHKVKQKPGKP
                     LLFGQFHRSLVFGLPGNPASVLTCFYAYVMPSLLKASGISHSNHYNKARLSEDLQKKP
                     GLTVFYKAVLQDDVVRILPQQESYILRSYALANCLVVVQEEDTLLHAGYEVSCLLIPL
                     MPV"
     gene            5309..6043
                     /locus_tag="PK742_08130"
     CDS             5309..6043
                     /locus_tag="PK742_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQU76600.1"
                     /translation="MEAGYVFYAALLIIAFLYASVGHGGASGYLALMALYSFSPLVMR
                     PTALVLNLFVAGIAFIHYYRNGYFKWRLFLPFAAASVPASFLGGLITLDEHIYRQVLG
                     VLLLFAVLRMLIRMKHGEKVVKEINRPLAFAIGGIIGLLSGMIGIGGGIILSPVLLLL
                     QWAGMKRTAAVSALFILVNSMAGLCGQLINGVDFYPEMWLMVTVAFAGGFVGSYIGAR
                     HTPPHFLRILLAMVLLVASFKLIFAL"
     gene            6040..6606
                     /locus_tag="PK742_08135"
     CDS             6040..6606
                     /locus_tag="PK742_08135"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HQU76601.1"
                     /translation="MKGLCISILAGGQSSRMGQDKGLTEWAGKCLIQHIIDAARPLSN
                     HIHIIANDAAYARLGWPVISDQYPGTGPAGGIATALLHSPFETNLILSCDIPRIDTGT
                     LQFIVATHKPGISVPIVNGQWQPLVAVYDHSIFHGWMMLLQKGERALHQMIRQLPHQE
                     VDMRSYPHYHEMAFQNMNTPEELRQAGA"
BASE COUNT         1639 a         1715 c         1690 g         1659 t
ORIGIN      
        1 tggaaagtag ataccgatgc tttcaaacag aaaggcataa aaggtgatgc cctccccttc
       61 ttccagatca tggcctgcat tgaccacgca tttattcagt acagcaaact gcagcaagac
      121 gatggcatag atggcacaca tcagccacca gtctatcagg aacggaccca cccacgcaaa
      181 taatgtgatg gccagatgca gccagaaggc tccgttgagt ggaatcagga tgggttcagg
      241 tctggttgtc atgcatcaaa aatacacgtt aggcaggaga gagcccgcac tacgaagctt
      301 atataactta tgtatcttag acgttctcaa cctgctatga aacatacact actgaccacg
      361 gtctttttta taggcactat tgccttgcaa gctcagacct gtccaggctg ttccattgat
      421 tactcctata ccagtccggg attgtatccg gagatcttac ctccggcaac agccggcgaa
      481 tattatgaag tggacctgac ctttctgctg caggaagaca ctacggtaga agtacttgga
      541 tctgatgtga cctttgcttt tctcaactac cacatcctgg aaccgatcgg tctgccttat
      601 ggcattcatg tgaccagcaa cctgggagat tttcctgttg actatgatcc ggccgatggc
      661 ttgttcggtt gtgcccgtgt ttgtggcacg cccctggtgg caggttccta taccatcact
      721 gttccgctca tcgccacgct ggaagatcct gcgggcgata atcctgctga atatgaactg
      781 agtctggaag ttctgccacc ttccgctact gatggcggta tcgtggcaag taccaccttt
      841 ggctgcagcc cgctcacggt cgatctcagt accggtcacc cgagtgacgg cgcaggaggc
      901 ttctcttata catgggactt tggcgacggc acttattcca tcctggagga tcctgtatcg
      961 gtcaccctca cagcagatgg aactgaaccg gtagagtacg tggtgaccca taccgtgacc
     1021 atcgacacga tcggatacaa cctggactat gtaacagtgc ttagtactgg atgcgatgat
     1081 tgtgctttct tcggttgcac cggcctgttt gccgaagaaa accccgacat ctatctgtac
     1141 ttcccggaac tgggtattac caccgactat tacctggata ccaatccacc agtcaccttt
     1201 tcgctgaata ccgcactgga tcccgctgcc acttacaata tgcaggtcaa agacgatgac
     1261 gacggtggct tcggcgctga cgataattgc ggaaacctga tgttcagtgg agatgatgtg
     1321 ggcgttttca cctatgacat cggtagcagt gatgtagaga tcagtatctc tcatccgatt
     1381 atcttctata cctatacaga tactatcgtg gtgtatccgg tgccctccat acccgaactg
     1441 actatcagcg gagagaccga actctgcgaa ggcgacagtg tattgctaag cacgactcct
     1501 gacccaactg tcacctacca gtggctgcag gatggagatc ccattccttc tgcctttgac
     1561 gccgattttt atgccatgga gacaggtacg tatgcactcc aggctactgc aacaggtgga
     1621 tgcacagcaa tcagtgaatc ggtggatgtg gtggtgtatg agctgccttt cccacccttc
     1681 gtaagtatca tcggcaacac gttacaaacc agctctgact atccgatcca atggtattac
     1741 agcggtgatc cgatacccgg tgcgaatgac agcatctatg agcctgccat cgaaggcata
     1801 taccaggctg ccgccatcaa cggaccatgt atcagctttt cttctgagat cgattttgta
     1861 tttcagtcag ttcttgacct tgatccattc cagcttacca tagcacccaa tccggtggat
     1921 gatatgttgt tcctctccgg taatacagca gttgcaggta ccatgaccat acagatcatc
     1981 aatgccacag gtcaggtcat aaggtcaaag accttgtctg taggatcagt attttcactg
     2041 cagttaccga tggatgaaat tccctcagga tattacagac tgatcatgcg atcggcagaa
     2101 caacaaggct cctatccttt cctgcgtcag tgatcatcac ccttgccaaa caggaaagtg
     2161 atcggaatat gcacccggtc atgccaggct tcttccgtat gcggtgcacc ttcaaagacc
     2221 agtgaaaggc tgttggcagc agtgtatccc ccttcccgca tgatctgatc agcggattgc
     2281 tggtatggcg catagaggct gtccagtgtt gtggtgccgt gatcgaaata cagtttgtgg
     2341 gtggatggat caggcaggtg atctttaaaa tatgccagca tagcaaaggg gaaagggttg
     2401 ttttcctgtc tgtacaatcc ggtccagtgt gtggacaaac aagccgctcc gccaaacact
     2461 tccgggtatt cacaaatggc gaacatggag atcagtccgc cgaaactgga acccatgatg
     2521 aacgtatcct ctctgccgga taaggtagga taatgactgt caatgtaggg cttgagttcg
     2581 cttaccagaa aacgcagata cagatctcct ttcagattat caccggtgaa caatcggccg
     2641 gtagccggtt catcccgcag gatggccata tcgctgtcgc tgaaatagac aaagggtttc
     2701 atagaggtgt attccaggta acgaaccggt gcattattcc agaccgccac caggatgaaa
     2761 gcttggatct gtccgctgtc gatcagtgca ggaatcactt catccatccc ccactcctgc
     2821 ttattccagg tagtgcgtgc atcaaacagc atctgtccgt cctgcatgta taagacagga
     2881 tactgttgcg tagtttcata gccgggaggc agccagatat ctacaggcct ggcatccaca
     2941 taactgctgg caaaactgtc gatgcgaatc agtgtaccgg cactcagttc cggcatagga
     3001 agttgtgctg cgagcacctg cgaaaggagc atcagcacca aaggaaatac gaaacgtcct
     3061 ctcatggttc taagatacat cgtattcggg cttcgaccgg aaagggctct tcccttacct
     3121 ttgtgatatg ctctccgata cattcggtcg caggcacgac tacttgcgca tatcgctgac
     3181 ggataactgc aatttccgct gccgctactg catgcccgaa gaggagatgc aattccaccc
     3241 cgatgagcag ctgatgcagc cggatgagat cgaggctctg gccaagatct ttgtgcaact
     3301 gggaacgagg aagatccggc ttaccggcgg tgaacccctg gtgcggaaag atgccggaaa
     3361 catcatcgaa agactggctg ccttaccggt acaacttact ttgaccacca acggtttcct
     3421 ggccgatcgc tacatcgatc tgttccatgc atcgaacatt cgttcattga atatcagcat
     3481 cgacagcctg tcgcccgaaa aattcctggc gataacaaga cgagacaagt tcgacacagt
     3541 atggaaaaac attctgacca tggtggaaca ggactttcat gtcaagctga actgtgtggt
     3601 gatggaaggg gtcaatgatg atgaattact ggactttgtg gcgctgacca aagacctgcc
     3661 cgtgcatgtg cgtttcattg agttcatgcc ttttaccgga aaccactgga acaacaaggc
     3721 ggtgttcaca tggcagcaga tgctggaaag gatctcagga aaattcctgt tcaagaagct
     3781 gtcggatgaa ccgaatgaca cggccaagaa atatcaggtg cccggtcatg caggcacctt
     3841 tgcggtcatc agcaccatga gtgccccgtt ctgttccacc tgtaaccgca tgcgactgac
     3901 cgccgacgga aaaatgaaaa actgcctgtt ttctgccgga gagacggacc tgttgacagc
     3961 attgcgcaaa ggtgatgatg tggaagccat gatcaggaaa aacctggatg aaaaacagga
     4021 aatgatgggc ggacaattca ccgattattc ggtcacaaac cctgatgaaa ttcaaaaccg
     4081 cacgatgatt tccatcggcg gatgacttac ctttaagcat tatgatctcc gttgacgaag
     4141 caattcaact ggtcatcgga cacacaccat ccccccggac catcacgctg ccgctgcagg
     4201 catgcgcagg acatgttttg gcacaggata tcctgtcgcc aggccccttc ccgtcttttg
     4261 atcagtcggc tatggatggt tatgcttttt gcttcgccga ctggcagcaa aatcccttac
     4321 ccgtagatgg tgagaccgcc gcaggacaat tccctgagcc cctcattcag ggcaccacca
     4381 gaaggatttt taccggagca gcgataccgc ccggtgctga tactgtagtg atgcaggaac
     4441 acgtacagac gcacgacggg gtcatacata tagaaaactc aacattgata gccggtgcca
     4501 atgtgcgcaa acagggatcg cagatccagg ccggagagtg tgccctgccc tctggtcata
     4561 cattacaggc tgcgggtatc ggattcctgg catccatggg atttgaccgg gcaactgttt
     4621 ttgaaaagcc tgtgatccat atcgtagtga caggttcaga actattgcca cctggtgctc
     4681 ctgcagaagc aggtaaggtc tatgaatcca acagtgtcat gctgctggct gccctgcgtt
     4741 ccatcggaat ggaagcgcag gccgtacacc acgtgccgga tgatccgcag gctacactgg
     4801 cattgctgga ccgactcagt acggaatgcg actatattct cgctacaggt ggggtatctg
     4861 tgggagatta tgacttcgtc ggacagatga aacaccatcc tgcgtttcaa agcatcttcc
     4921 acaaggtgaa acaaaaaccg ggaaagcctt tgctgttcgg acaatttcat cgttcgctgg
     4981 tgtttggcct gcccggaaat cccgccagcg tgctcacctg tttctatgcg tatgtgatgc
     5041 cgtcattgct aaaggcatca ggcatctcac atagcaatca ctacaacaag gcaagacttt
     5101 cagaagacct gcagaagaag ccgggcctga ccgtttttta caaggcagta ctacaggatg
     5161 atgtggtcag gatactgccg caacaggagt cctatatcct ccgcagttat gccctggcca
     5221 actgtctggt ggtggttcag gaagaagata ctttgcttca cgccggctat gaagtttcct
     5281 gtttgctcat tcctttaatg cctgtctgat ggaagccggt tatgttttct acgccgcact
     5341 actgatcatt gcatttttgt atgcatcggt cgggcatggt ggtgccagtg gttacctggc
     5401 gttgatggca ttatatagtt tttcgcccct cgtcatgaga cccactgccc tggtactgaa
     5461 cctttttgtg gcaggcattg cattcataca ctattaccgg aacggatact tcaaatggcg
     5521 tttgttcctt ccctttgcgg ctgcctccgt accggcatca tttctgggtg gcctgatcac
     5581 actggatgaa catatctacc gtcaggtact gggtgtactg ttgctgtttg cggtgctgcg
     5641 catgcttatc cgcatgaagc acggcgaaaa ggtggtgaag gaaatcaatc gcccgctggc
     5701 atttgccatt ggaggcatca tcggattgct gtctggcatg ataggaattg gcggtggtat
     5761 cattctcagt cctgtgttgt tattgttgca gtgggccggc atgaaacgta cggcagctgt
     5821 aagtgcgctg ttcatcctgg tgaactcgat ggcagggctt tgcggacagc tcatcaacgg
     5881 ggtggatttt tatcccgaaa tgtggctgat ggtgaccgtc gcttttgccg gtggatttgt
     5941 gggttcttac atcggcgcca ggcatacccc tccgcatttt ttgcgtatct tactggcaat
     6001 ggtgctgttg gtcgcatcat tcaaactgat ctttgcctta tgaagggtct gtgtatctcc
     6061 atactggccg gtggccagag cagcagaatg ggtcaggata aaggcctgac agaatgggct
     6121 ggtaagtgcc tcattcagca catcattgat gctgcacgtc cgcttagcaa ccacattcat
     6181 atcatcgcga atgacgcagc ttatgcccga ctgggctggc cggtcatcag cgatcagtat
     6241 ccgggcaccg gtccggcagg cggcatagcc acagcgttgc tccacagtcc gttcgagacc
     6301 aatctgatac tgtcctgcga tatacccaga atagatacgg gtactttaca attcatcgta
     6361 gccactcaca aaccgggtat cagtgtaccg atagtgaatg gtcaatggca accattagtg
     6421 gcagtgtatg atcacagtat ctttcacggc tggatgatgt tattgcagaa aggagaaagg
     6481 gcattgcacc agatgatacg gcagttacca catcaggagg tcgatatgcg ttcctatccg
     6541 cactaccatg aaatggcatt tcagaatatg aatactccgg aagaacttcg acaggcaggg
     6601 gcatgaaaat acagatcaga acctttggtc cgctgaccga tcacctaaca gatacagaac
     6661 tggaatattc cggtgaacct accatcgcag ctctcagaag att
//
LOCUS       DAOMAX010000140         6677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1786_length_6677_cov_2.838417, whole genome shotgun sequence.
ACCESSION   DAOMAX010000140 DAOMAX010000000
VERSION     DAOMAX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_6677_cov_2.838417"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PK742_08140"
     CDS             complement(<1..323)
                     /locus_tag="PK742_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76602.1"
                     /translation="MSNIVKFDIKQAPHAFDEVLELQIGSWAVPAGEITVEQYLKDCV
                     AAVSDLKIADVNDKEGYEIVSRARKDLAKLRTTLEKTGKAARDNANAFAKAVIAEEKR
                     LISIPS"
     gene            complement(320..1270)
                     /locus_tag="PK742_08145"
     CDS             complement(320..1270)
                     /locus_tag="PK742_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76603.1"
                     /translation="MSNELQKAEAPQMALADRLLTNSQTIEDAMKVATMIKKSGFCPE
                     HLDEGGIIIAVSAGQALGLGLMQSMVDIVPIKGLPTLKGDLIRSLIMPNKSICEVWEE
                     SYEGPEPDYTKGPDGFKDDFCHVLKVQRVGQKERIFRFSVLQAKQMGLWITVGEATKE
                     PKLKHIGWYKTPQRMLMYRNIGFAGRDVWGDILRGFKSYEEVLDYDTDAPIPQKAQTG
                     PVAQKPAQKKAEEVVETVELLFADGADFSFLDDFKNTPRDLAMLAKMNAAIAKAAKVS
                     AKVATASLQNAIKGQDLDATEILTEGQIGDIVNILKLATK"
     gene            complement(1263..1595)
                     /locus_tag="PK742_08150"
     CDS             complement(1263..1595)
                     /locus_tag="PK742_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76604.1"
                     /translation="MAIKPYSPQLVQQALTLAKWAEQQQQAINATNFPNEEARQKRQA
                     GMDVIADGVEYIQTSLMLMDKMLPFLRRLHDNLEIEGALYMDTELVVNKPMREELSDI
                     LKNFDTNE"
     gene            complement(2134..3276)
                     /locus_tag="PK742_08155"
     CDS             complement(2134..3276)
                     /locus_tag="PK742_08155"
                     /inference="COORDINATES: protein motif:HMM:NF021128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HQU76605.1"
                     /translation="MSKILKAHHKLHKIPARTPEWHLFRRRSGLGGSDVGAAVGDNPY
                     FSRWHLWETKVGLRNDNLEPNEKILHGLIDENQTAKLWGYYDGYVNDSGVPTYVDRYM
                     QILENPKDRELRTLLRQRRSERVNGVVTHSSYPWLFMNLDRIIPAGQFSFKPGRVPLE
                     THAPLECKSMGEFVGRHYDESIPPYYHSQGHSYMILLDTDYVEFAVRLGLSLQVKPFL
                     FDRDYADRLLAETYDFWHRYVVPAQAYMERREQLLASQDYRGAEIIEGQMREHEPPPD
                     GTSLSLDYRKERIAVEQGGTGDRKGDQFTATLDNTHDMFSLGIELLDISSAKKVTEAK
                     ETKIKDEMLAFFRNTGMNKLDFGASGYISYYPNKNGVFTLRNQIKV"
     gene            complement(3273..3932)
                     /locus_tag="PK742_08160"
     CDS             complement(3273..3932)
                     /locus_tag="PK742_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76606.1"
                     /translation="MPTKRLIVTPGINKLLQSGVATTVYKLLKGDVNENMKYGWYGQV
                     GDKFYVTETCLISKDSTEILYENGDYARRVGRALDVSEGYSLAYHEAQALGWKEHFPN
                     ESPRWTARYWYEIEEITYPVRVQLLDAGMLEDLGIRQVDPNEYEFAEIKVTARNITEA
                     WRAVWNHTHNRWKTHGSRDQFVSYPFKEEDKIPSPKGMSITDHQWVTNPFIQIYKLKV
                     L"
     gene            complement(3892..4287)
                     /locus_tag="PK742_08165"
     CDS             complement(3892..4287)
                     /locus_tag="PK742_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76607.1"
                     /translation="MFVTSKDYIRLRKTFRLGNIASPKQFAEICEVPEAKIRALFRAT
                     ALRNRMKHPQLTAIEPFTSIGDPGEGGGGIFIVLDEHAHKRMKHYAELAGFDQETFIR
                     RAQRAGFEFLIKIEEEDANKEADSNTGDQ"
     gene            complement(4327..5010)
                     /locus_tag="PK742_08170"
     CDS             complement(4327..5010)
                     /locus_tag="PK742_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76608.1"
                     /translation="MDVVIPLGSGSRWQDNELRFCLRAVEKHLKGYDRIYIVGRKPDW
                     IQNIEFIPYVEAHTRERNIMEKVRRACKSGVSDNFLFMNDDHFLIKDVDAHTYPYYYN
                     GPIAPYGGEYGQSLYRTQRELSTRGLPTLNYDIHCPIIYSKRLFLDIMACYSWKKDLV
                     VKSLYANTAGVEGVQMNDCKINRNIGVAVLRQVEQDRHVFSIGDGAIRLALKKYFAEK
                     LPHPCRYEK"
     gene            complement(5010..5588)
                     /locus_tag="PK742_08175"
     CDS             complement(5010..5588)
                     /locus_tag="PK742_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQU76609.1"
                     /translation="MPGIINHKRKTVYWYIPKVACTSMKLAMAEDMGIAIPDGKVHAD
                     GVFEITNDFNAYSDYFHFTIRRNPVERALSLFNDKIRPDRLTNDTYTDGIENVVFAGF
                     QQWFWPAMTFEQFVLILASLRLQDPHIAPQWTQHPQVNIYVIWIESLSLELKKVVPWL
                     PPLGIHNQSVKRGEVTELARLTIRHHYAKDFL"
     gene            complement(5591..6250)
                     /locus_tag="PK742_08180"
     CDS             complement(5591..6250)
                     /locus_tag="PK742_08180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU76610.1"
                     /translation="MDKLSMVAHELKFWAGFVKTERFLDGWCGNKPTPELHKEVEEFI
                     QEYATTHGKTMALDVGSGVVSILNGVHSDVDVVATDLLGEFYEVIFDYEQVKLNPPIA
                     VGGEQLSTILLPEERYDVVHCSNALDHCQDPKEVLWQMAACCKRGGYVIIQGFENEGE
                     AERYQGFHQWNITLPDTELYVQGHEDEYYLVAPNLTPYLYLTKEIQNGKRWYIWIANK
                     L"
     gene            complement(6238..>6677)
                     /locus_tag="PK742_08185"
     CDS             complement(6238..>6677)
                     /locus_tag="PK742_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76611.1"
                     /translation="AGVLLEGMEGWGAVHPAFSSDHRHLWPGDEIGIVEVPYVEWTAP
                     MVDASLFAKIQLDRRMPYWGMDLDWSYRARQLGYRLGCSHDVVIGHEYIRNNPKPHQV
                     TIKRRRMRRQTDQPTHQQLIRKYGRDWRNVLGYTDTKKTIWTN"
BASE COUNT         1470 a         1870 c         1541 g         1796 t
ORIGIN      
        1 gagggaatgg agatcaaacg cttttcctcg gctatgacag cttttgcgaa ggcattggcg
       61 ttatccctcg cagccttgcc ggtcttctcc agggtagtcc tgagtttggc gagatccttt
      121 ctggcacggc taactatctc atagccctcc ttgtcattga cgtctgcgat cttcaggtcg
      181 ctcacagcgg ccacgcagtc cttcaggtat tgttccacag tgatctcgcc ggcaggtacg
      241 gcccaagaac ctatttgcag ctccagcact tcatcgaatg catggggagc ctgcttgata
      301 tcgaatttca caatgttgct catttcgttg ccagtttaag aatgttcaca atatcgccta
      361 tctgtccttc ggtcaggatc tcggttgcat caaggtcttg tcctttgata gcgttctgca
      421 gggatgccgt agcaactttt gcagacacct tagctgcctt ggcaatggct gcattcatct
      481 tcgccagcat cgccaggtcc cgcggcgtgt tcttgaaatc gtccaggaag gagaagtcag
      541 caccgtcagc gaacaacagc tctacggttt caacgacctc ttccgccttt ttttgtgccg
      601 gcttttgagc cacagggccg gtctgcgcct tctgagggat cggtgcatca gtgtcgtaat
      661 ccagcacctc ttcataggac ttgaagcccc ggaggatatc accccatacg tcacgaccgg
      721 cgaaaccgat attcctgtac atcagcatac gctgaggcgt cttataccag cctatgtgct
      781 tgagttttgg ctctttggtg gcttcgccca cagtgatcca cagccccatc tgcttggcct
      841 ggagaacaga gaaccggaag atcctctcct tctgacctac ccgctgcacc ttcagtacat
      901 ggcagaaatc gtctttgaat ccatcaggac ctttggtgta atctggttcc ggtccctcat
      961 agctttcctc ccacacctca caaatactct tgttcggcat gatcagggag cggatcaggt
     1021 cgccttttag tgtcggcaag cctttgatgg gcacgatgtc caccatagac tgcatcaggc
     1081 caagccccag ggcttgtccg gcagacacag cgatgatgat accgccttca tcaaggtgtt
     1141 ccggacaaaa cccggacttc ttgatcatgg tcgccacctt catggcatct tcaatggttt
     1201 ggctgttggt caacagccgg tcagccaacg ccatctgagg cgcttcagct ttctgcagtt
     1261 cattactcat ttgtatcgaa atttttaagg atgtcagata attcttctct catgggcttg
     1321 ttcaccacaa gctcggtgtc catatacagc gcaccttcta tctccaggtt gtcatggagc
     1381 cggcgcagga acggcagcat tttatccatc agcatcagac tggtctggat gtactccacg
     1441 ccatcggcga taacatccat gccggcctgt cgcttctgac gggcttcctc atttgggaag
     1501 ttcgtggcat tgatagcctg ctgttgctgc tctgcccact ttgcaagcgt cagggcctgc
     1561 tgtacgagtt ggggcgaata gggtttgata gccattgctt attaattcta tttactgggt
     1621 gaacaaatcg taatcctagc attgaaaaca ccgactcttc ggtcgggaaa ttgcaaatct
     1681 tgatgtcgct cttgggcatg taggttctgc ccttcccttt caccgcctct gctttcaggc
     1741 acaacccata gggcgtgtcc acaaatccca gacttctcca tctgctcctg atccactccg
     1801 caaactccgg agtgccggta cggaggatct gctgacgccc gaggtcacgc atcgaggtca
     1861 ggtaaatcct tccagggatg gtcccatcgc aattgatatg tgtgagtgga acctcgcata
     1921 caaacctgtt cggccctgca ggacgaatgg ttgaggcaac tccgggtagg ttctcatgaa
     1981 ctctcatccc atagtcagga actaccagta catctattgt ggacacgccg cctgtgtaca
     2041 gtctgacatc ccctactatt tcccagcgaa tgaatcgcgg agaagtcaga taggtttttg
     2101 ccaactgcat ggcttcaaat gggcgaataa acttcatacc ttgatctggt tgcgaagggt
     2161 gaaaacaccg ttcttgttgg ggtaatagct gatatagcca ctggctccga aatcaagctt
     2221 attcataccg gtattcctga agaaagccag catttcatcc ttaatcttgg tttcctttgc
     2281 ctctgtgacc ttctttgcac tggatatatc cagtaactcg atgccgaggc tgaacatatc
     2341 atgggtgttg tccagtgtag cggtaaactg gtcgcccttg cgatctccag tacctccttg
     2401 ctctactgca atcctttcct tccggtaatc cagagaaaga ctggtcccat ctggaggtgg
     2461 ttcatgctca cgcatctgcc cctcgatgat ctccgcacct ctgtaatcct gagacgccag
     2521 aagctgctct cggcgttcca tgtacgcctg tgccggtaca acgtatctgt gccagaagtc
     2581 gtaggtctct gccaacaatc gatcagcata atccctgtca aacaggaacg gctttacctg
     2641 aagggaaagg ccgagcctga cagcaaactc cacatagtca gtgtccagga gaatcatata
     2701 gctatggccc tggctgtgat aatatggcgg tatgctttca tcgtagtggc gaccgacaaa
     2761 ctctcccatg ctcttgcact ccagaggggc atgtgtttcc agtggcactc ttccaggctt
     2821 gaagctgaac tggccggcgg gtataatcct gtccaggttc atgaacagcc aaggatagct
     2881 ggaatgagtg actacaccat tgactctctc cgatctccgc tgcctcaaca gggttctgag
     2941 ctccctgtct ttcgggttct ccaatatctg catgtaccgg tcgacatagg tgggcacgcc
     3001 actgtcattg acatagccat cataataccc ccacagtttt gccgtctggt tctcgtcaat
     3061 aaggccgtgc aggatcttct cattcggctc caggttgtca ttccgcaacc ctactttggt
     3121 ctcccacaaa tgccaacgag agaaatacgg gttatcgcct acggcagcgc cgacatcgga
     3181 tcccccgaga ccgctgcgcc tccggaacaa atgccattct ggcgttctgg ccgggatctt
     3241 atgcagcttg tgatgggctt tcaggatttt gctcacagta ctttgagttt atagatttga
     3301 atgaatggat tggtcaccca ctggtgatca gtaatagaca tccctttggg tgacgggatt
     3361 ttatcctcct ccttgaaggg atagctgaca aactgatctc tgctgccatg tgtcttccag
     3421 cggttgtgag tatggttcca taccgcccgc catgcctcag tgatattcct ggcggtcacc
     3481 ttgatctcag caaattcata ctcattggga tcaacctgac ggattcccag gtcttccagc
     3541 attcccgcat ccagcagttg gactcggacg gggtaagtta tttcctcgat ctcataccag
     3601 taccgggcag tccaccgcgg ggattcattg gggaaatgct ctttccatcc cagcgcctgt
     3661 gcctcatggt atgcaagaga gtatccctcg ctcacatcaa gcgccctgcc tactctgcgg
     3721 gcgtagtcac cgttctcgta aaggatctca gtgctgtcct tactgatcag gcaggtctcg
     3781 gtgacataaa acttatctcc cacttgcccg taccatccat acttcatgtt ctcgttcaca
     3841 tcccccttca ggagtttgta aactgtcgta gccacaccgg actggagcag cttattgatc
     3901 cccggtgtta ctatcagcct ctttgttggc atcttcttct tcaattttga ttaggaactc
     3961 gaaacctgcc cgttgagctc tgcggataaa cgtctcctgg tcaaagcctg ccagctctgc
     4021 ataatgcttc attctcttat gtgcatgttc gtccagcaca atgaagatac caccacctcc
     4081 ttctccggga tcaccaatgc tggtaaacgg ctcaatagcc gtcagctgcg gatgtttcat
     4141 ccggttacgg agtgcggttg cacgaaataa agctctgatt tttgcctccg ggacttcgca
     4201 gatttctgcg aactgcttag gagaagcgat attgccgagg cgaaaggtct ttctgagacg
     4261 aatataatcc ttagaagtaa cgaacattca gattcaattt cagattcatc gttacaaatc
     4321 tgaaatttat ttttcgtacc tgcaaggatg gggcagtttc tcagcgaagt attttttcaa
     4381 cgccagccgg atagccccgt ccccgatact gaacacatgc cgatcctgct caacctgtcg
     4441 aagtaccgcc acacctatgt ttcggttgat tttgcagtca ttcatctgca ccccttctac
     4501 gccggctgta tttgcatata ggctcttcac tacgagatcc tttttccaag aataacacgc
     4561 cataatgtca agaaataggc gtttgctgta aattattggg cagtgtatgt cataattgag
     4621 agttggaagc ccccgtgtgg ataactctct ctgggttcgg taaagactct ggccatactc
     4681 cccaccatac ggagcgatcg gaccattata ataatagggg taggtatggg catccacgtc
     4741 cttgatcagg aagtggtcgt cattcatgaa caggaagttg tcgctgactc cagacttgca
     4801 cgccctccgc accttctcca tgatattccg ctcccgggtg tgcgcctcta catatgggat
     4861 aaactcgatg ttctggatcc agtccggctt cctaccgaca atatagatcc tgtcataccc
     4921 tttcaggtgc ttttcaactg ctcgcaggca gaacctcaac tcattatcct gccaccggct
     4981 accgcttcca agtggtatga ctacatccat tacaggaaat ctttagcgta gtggtgtctg
     5041 atggtgagtc tggccagctc tgtgacctcg ccccgcttca cgctctgatt atgtatgccc
     5101 aaaggcggta gccatggtac gaccttcttt agttccagac ttaggctttc tatccatatc
     5161 acataaatat taacctgtgg atgctgagtc cattgcgggg ctatatgcgg gtcctgcagg
     5221 cgcaaggacg ccagtatcag cacaaactgc tcaaaggtca ttgcgggcca gaaccattgc
     5281 tgaaaaccag cgaacactac attttcgatg ccatcggtat aggtgtcatt ggtcagccga
     5341 tccggacgga tcttatcatt gaacaggctg agcgctctct ccaccgggtt cctcctgatc
     5401 gtgaagtgaa agtaatcgct gtacgcattg aagtcattgg tgatctcgaa cacgccatca
     5461 gcatgcactt tgccatctgg aatcgcgatg cccatgtcct cagccatagc cagtttcatg
     5521 gacgtacacg ccacttttgg tatataccaa taaaccgtct ttctcttgtg attgatgata
     5581 ccaggcataa tcagagttta tttgcgatcc atatatacca gcgtttgccg ttctggatct
     5641 ctttggtgag ataaagatag ggcgtcaggt tcggagccac caggtaatac tcatcttcat
     5701 ggccctgaac atatagctct gtatcaggaa gagttatgtt ccactgatgg aagccctgat
     5761 accgctctgc ctctccttcg ttctcaaagc cttggataat gacgtacccg ccccttttac
     5821 agcatgcagc catctgccac aacacctcct tcgggtcctg gcaatgatca agggcattgc
     5881 tgcagtgcac gacatcatac cgttcttccg gcagaaggat agtgctcagt tgctcacctc
     5941 ccacagcaat cggtggattc agtttcacct gctcgtagtc gaagatgacc tcgtagaact
     6001 caccgagcag atcggtggcc actacatcta catctgagtg cacgccattg aggatgctta
     6061 ccacgccaga accgacatcg agggccattg tcttgccgtg agtggtggca tattcctgaa
     6121 tgaactcctc tacctcctta tgaagctcag gggtaggctt gttcccacac cacccatcca
     6181 ggaagcgctc tgtctttaca aatcctgccc agaacttcag ctcgtgggcg accatgctta
     6241 atttgtccat atagtttttt tagtgtccgt gtatcctaat acattcctcc agtccctgcc
     6301 gtacttcctg atcagctgtt gatgtgtggg ctgatctgtc tgccggcgca tacgtcgtct
     6361 tttgattgta acctgatgtg gcttcggatt gttgcgaata tactcatgcc cgatcaccac
     6421 gtcatgactg caccccaacc ggtagcccag ctgacgggcc ctataactcc agtccagatc
     6481 catgccccag tacggcattc tccgatccag ctggatcttc gcaaacaaac tggcatctac
     6541 catcggagcc gtccactcca cataaggaac ttccactatt ccgatctcgt cacctggcca
     6601 aagatgccgg tggtcactgg aaaatgcggg atgtacagcc ccccagcctt ccatcccctc
     6661 cagcaacaca ccggctt
//
LOCUS       DAOMAX010000141         6591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1837_length_6591_cov_4.047736, whole genome shotgun sequence.
ACCESSION   DAOMAX010000141 DAOMAX010000000
VERSION     DAOMAX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_6591_cov_4.047736"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PK742_08190"
     CDS             complement(<1..1153)
                     /locus_tag="PK742_08190"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQU76612.1"
                     /translation="MKHIYKGLFRVLILTPLLGSIPAYSQILIDDTYTPEELAESLIG
                     TGVTISDVTIDCHAGGFAFFNCVDCNVGMDSGIVLVSGPASEVAGPNNASGTGMSMGG
                     GGDPDLEDLTTSTTFDACIIEFDVLVNSDSLQFDYVFGSEEYTTYVNSSFNDVFGLFI
                     SGPGIVGLQNLALIPTTTIPVAINNVNPLEYEEYYIDNGVGCTPFGMSCSEGFLTAPY
                     TTDDYYIEWDGFTTVLTARTAVTPCETYHLKIGVSDAGDGILDSGVFIKAGSLSSPGV
                     TISYDYDITGYPEMIEGCNNGELVLSLSFAAVDTIVVNLEVSGTATNGLDYSSIPTFV
                     TFYPGDTSVVIPIEAFTDATAEGVETIVITGELTCAIASGDSIVLFIADS"
     gene            complement(1207..3042)
                     /locus_tag="PK742_08195"
     CDS             complement(1207..3042)
                     /locus_tag="PK742_08195"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HQU76613.1"
                     /translation="MCRRNLLFTALFTGICLASSAAFGQLVVDGSPSAEDMAEALVGE
                     GVSISDVELDCPSGAFGFFECVDCNVGIPNGIILTSGSIDEAVGPNNSGSAGASTFTP
                     GDGDLEDYIGATTNDACILEFDLTVTSDTLSFNYVFGSDEYLEWVGSFNDGFAFFISG
                     PGIVGLENLALIPGTSDPVTINNVNDFSNPEFYVDNGSGFEAPYSTDDYYIQYDGFTT
                     VLTAVREVIPCETYHLKMVIADAVDNAYDSGVFIEAGSLSSPGVTIEYDVDIDGYLDL
                     IEGCNNGYLTFDLSYPAVDSLWVPVTVSGTATNGVDYTTIPDSVLFEPGDTTYTIPIE
                     IIEDAIDEGLETIVLYVELGCAIGFGDSLVINILDYLPIEVSADTIICPGGNAVLSAT
                     GADTYTWSPAAGLSSTTGATVNASPDEPTTYSVEGTLYACVSSSEIFVDVEFPVATAD
                     PDTTIFFGETATLGATGGVSYNWTPAGTLDNPNAQFPNAFPLETTTYNVTITTALGCT
                     ATDQMTVFVSGNAEVWVPNAFSPNNDGYNDNFTIVVRGQLSFFELAIYNRWGEQVYLG
                     SLGSAGWDGMYNDIEQPMGAYVYTLKWTDTEGNTTQEQGQFTLVR"
     gene            complement(3174..4469)
                     /locus_tag="PK742_08200"
     CDS             complement(3174..4469)
                     /locus_tag="PK742_08200"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQU76614.1"
                     /translation="MTILRAYATLGELISHLTGAHINLPIFSFGFFVAIAFLVAAWLL
                     SKELARKEQEGLLHPIPEKQVVGLPPKPAEIILNAAIGFVLGFKVGAIIADYATFSAN
                     PQAFVFSAEGSWLGGIMGLVLLGWLKYQERKKQQLPRPEERTVQVYPHQRVGDIVVIA
                     AVTGILGAKIFSNFEEPGGWQQFWQDPMGNFFSGLTIYGGLLLGAASVIWFASRKKIS
                     VLHLGDAVAPALILAYGIGRIGCQVAGDGDWGIYNTAYMTMEDAGIQHAEPGNFDSVV
                     AMYPRYFDYSFKGMEEVPHGQFKGPDFLPRFLFAQNYAHNVNGDGLPIPGCEGPFCAV
                     LPVPVFPTPLYEILMSLAIFGVLWVLRRRMRFAGGLFALYIFFNGLERFWIEKIRVNN
                     VLEFAGIRATQAEFISVGFMLFGLAFMAFLWQRSRNRTV"
     gene            complement(4466..5827)
                     /gene="mtaB"
                     /locus_tag="PK742_08205"
     CDS             complement(4466..5827)
                     /gene="mtaB"
                     /locus_tag="PK742_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQU76615.1"
                     /translation="MQRTVSFYTLGCKLNFSESSSLSRQMEEAGYRLVPFQQGADVYV
                     INTCSVTDHADKKCRKVVRQAKKFRPDARVAIVGCYAQLKPKEIAEIDGVDLVLGAAE
                     KFNLPQHLNNLTDTTETSVIAGPIDVVKDFLPAYSFQDRTRTFLKVQDGCDYQCAFCT
                     IPLARGKSRSGTIEQVRRQAEDAAANGVKEIVLTGVNIGDFGRREGVLPEDRETFLQL
                     IQTLDELKGIERFRISSIEPNLLSDDIIRFVAGSKRFVPHFHIPLQSGSDKILKAMGR
                     RYKKDLYAHRIQLIRDLMPDAGIGVDVITGFPGESHADFLETYHFLQELDVSYLHVFT
                     YSERPDTRAIKMADPVPGAVRQERTTQLISLSEKKKRAFYQKYSGTMRKVLLEAETDG
                     IWMTGFTDNYIRVRTAFDPLLVNELAWCGLDELLPDTQFECQTPVPVSDESLILNNVS
                     KSV"
     gene            5891..>6591
                     /locus_tag="PK742_08210"
     CDS             5891..>6591
                     /locus_tag="PK742_08210"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQU76616.1"
                     /translation="MGMLARIGAYEYDLEQGFAICIPFHQGPDQVNCYYAPPFSVSPV
                     VMGDFTGEISSGAPVNYRNISVNPHGNGTHTECLAHISDADLDVHAALTDSFFPVQVL
                     TIAPHRLTNGDQVLRFREAEILPEIKALAIRSLPNTSDKQHRAYSGTNPPYLDAACVA
                     LLPDLGIDHLLIDLPSVDKEEDGGALAAHKAFWNFASVKRTHATITELIFIPDHIKDG
                     RYLLNLQIAPFRTDA"
BASE COUNT         1778 a         1653 c         1483 g         1677 t
ORIGIN      
        1 aactgtctgc aatgaacaaa actatgctat cgccgctggc aattgcacag gtcagctcac
       61 ctgtgatgac aatggtttct acaccttctg ctgtggcgtc cgtaaaagcc tcaatgggaa
      121 tgaccacact ggtatcgccg ggataaaaag ttacaaatgt tggaatagat gaataatcca
      181 gaccgttggt ggcggtaccg gaaacttcca gattcactac tatggtatca actgcggcaa
      241 agctgaggct caacaccagt tcgccgttgt tacagccttc tatcatttcc gggtatccgg
      301 ttatatcata atcataggaa atggtaacac ccgggctgct gagactacct gctttgatga
      361 atacaccgga atccagaatg ccatcgccgg catcagacac accgatcttc aggtggtagg
      421 tttcgcaggg agtcactgca gttctggcgg tcagcaccgt ggtgaagcca tcccattcaa
      481 tataataatc atcagtagtg taaggcgccg tcagaaaacc ttcagaacag gacatgccaa
      541 aaggcgtaca tccaacacca ttatctatgt agtattcttc gtactccaac gggttgacgt
      601 tattgatggc aaccggaatg gtagttgtag gaatcaaagc cagattctgc aggcctacga
      661 ttcccggtcc ggaaataaaa agtccgaata catcattgaa gctggagtta acgtaagtgg
      721 tatattcttc agaaccgaat acataatcga actgcagaga atctgagttt accagtacat
      781 caaattctat gatacaggca tcaaaagtgg tagaggtggt gaggtcttcc agatccggat
      841 ctccacctcc tcccatactc attcctgtac cagaggcatt gttcgggcct gcaacttcag
      901 aagcaggtcc agacaccagt acgattcctg aatccattcc cacattacaa tctacacagt
      961 tgaaaaaagc aaatcctccg gcatggcaat caattgttac atcactgatg gtcacacctg
     1021 tcccaatgag tgactctgcc agctcttcgg gtgtataggt gtcatctatg aggatctgtg
     1081 aataagccgg gatacttccc aatagggggg tgaggatgag tacccggaaa aggcctttgt
     1141 aaatatgctt catggcatct ccggatttaa tgatgatatg tttgcgttag tctgcaaatt
     1201 cttttcttat ctgaccaatg tgaattgccc ttgctcctgt gtagtgttac cttctgtatc
     1261 tgtccacttc aaagtatata cataagcccc cataggctgt tctatatcat tatacatgcc
     1321 atcccatccg gcactgccaa gagagcccag ataaacctgt tcaccccatc tgttgtagat
     1381 ggcaagttca aaaaatgaca actgccccct aaccactatg gtaaagttat cattgtagcc
     1441 gtcattgttg ggtgaaaatg cattgggcac ccatacttca gcattgccac tcacaaatac
     1501 tgtcatctga tctgtagctg tacaacccag agcagtagta atcgtgacat tataggtagt
     1561 cgtttcaagc gggaaggcat tggggaattg tgcgtttgga ttgtccagtg tacctgccgg
     1621 tgtccaattg tagctgactc cgcctgttgc acccagggta gctgtttctc caaagaagat
     1681 ggtagtatca ggatcagcag ttgcaaccgg aaactccaca tcaacaaaga tctctgatga
     1741 gcttacacaa gcatacaagg ttccttctac tgaataagtt gtaggttcat ccggcgaagc
     1801 attcaccgta gctcctgtag tagaactcag acctgcagca ggtgaccagg tataggtatc
     1861 tgcaccggtt gctgataata cagcattgcc accaggacaa ataatagtat cagcactgac
     1921 ttcaataggc agatagtcaa ggatgttaat gaccaggcta tcgccgaatc cgatcgcaca
     1981 acctaattcc acgtacagta cgatggtttc caatccttca tcgatagcat cctcaatgat
     2041 ctctatagga atggtataag tagtatcgcc gggttcaaac aggacagagt ctggaatagt
     2101 ggtatagtcc acaccgttgg tagcagtgcc gcttacagta acgggcaccc agagtgaatc
     2161 aactgccggg taagacagat caaatgtcag gtaaccgttg ttacaacctt ctatcaggtc
     2221 cagatagccg tcaatatcga catcgtattc aatagtaaca ccggggcttg acaaagatcc
     2281 tgcctcaatg aatacacctg aatcgtatgc attatcaacc gcatctgcga tcaccatttt
     2341 caggtgataa gtctcacatg gtataacctc cctgacagct gtcagcacgg tagtgaatcc
     2401 gtcgtactgg atatagtaat catctgtact gtatggcgct tcgaaacctg atccgttatc
     2461 cacgtaaaac tccggattag aaaagtcatt aacattgtta atggtgaccg gatctgaagt
     2521 tccgggaatc agcgccagat tctccaggcc tactattccg ggacccgaaa taaagaacgc
     2581 aaatccatcg ttaaatgaac ctacccattc cagatattca tcggaaccga acacatagtt
     2641 aaaacttaat gtgtctgagg ttactgtcag atcgaattcc aggatacaag catcgtttgt
     2701 agtagcccca atgtaatctt ccaggtcgcc atctccgggc gtaaacgtag atgcgcctgc
     2761 agatccagaa ttgtttggcc ctacagcttc atctatagat ccggaagtga ggatgatacc
     2821 atttgggatc cctacattac aatccacaca ttcaaaaaaa ccaaatgctc cactggggca
     2881 atccagttcc acatctgaaa tggaaacccc ttcacctacg agggcctcag ccatatcttc
     2941 tgctgacgga gaaccgtcaa caaccagctg accgaatgct gcagatgaag ccaaacaaat
     3001 gcctgtgaac aaggcggtaa agaggaggtt tctccgacac atagagtccg aaatgatttg
     3061 aaatcaaata attatcaaat ttagagaaaa ggcctgaaat aatataaccg gaggcctgaa
     3121 aagccttctc ctttatgacg ctggcatgac ctaatgaaga tagtctgtat atatcagacc
     3181 gtccggtttc gggatcgttg ccagaggaag gccataaatg ccaggccgaa cagcataaat
     3241 cccaccgaga tgaactctgc ctgggtagct ctgatcccgg caaattccag cacattgttg
     3301 acacggatct tctcgatcca gaatcgctcc agtccattga agaaaatata caaagcaaag
     3361 aggcctccag cgaatctcat tcttctgcgc aagacccaaa ggacaccgaa aatggccagg
     3421 ctcatcagta tctcatagag tggggttgga aagaccggaa ccggcaatac ggcgcagaaa
     3481 gggccttcac aacccggaat gggcaggcca tcgccattga cattgtgggc atagttctgg
     3541 gcaaacagga agcggggcag aaaatccggg cctttgaact gtccatgcgg cacctcttcc
     3601 atacccttga aagagtagtc aaaatatctt gggtacatgg ccaccacaga atcaaaattt
     3661 ccgggttcag catgctgaat gccggcatct tccatggtca tgtaagctgt gttataaatg
     3721 ccccagtcgc cgtcgccggc cacctgacat cctattcgac caataccata cgccaggatc
     3781 aatgcgggag ccacggcatc ccccagatgc agaacggaga tcttttttct gcttgcaaac
     3841 cagatcactg aagcagcgcc cagtaatagt ccgccgtaaa tggtaagtcc gctaaaaaaa
     3901 ttacccatcg gatcctgcca gaattgctgc cagccgccag gttcttcgaa attggaaaat
     3961 attttggccc ccaggatgcc ggttaccgca gcgatcacta caatatctcc cactcgctga
     4021 tgtggataga cctgcactgt tctttcttcc ggccggggaa gttgttgttt ctttctctcc
     4081 tggtatttca gccatcccag aagaaccaga cccatgatgc cccccagcca acttccttca
     4141 gctgaaaaaa caaacgcttg tgggttggcg ctgaaagtgg cataatcagc tattatagcg
     4201 cccaccttga accctaaaac aaaaccgatg gctgcattca gaatgatctc agctggtttg
     4261 ggaggaagac caactacctg tttctcgggt atgggatgaa ggagcccttc ctgttctttt
     4321 ctggccagtt cttttgacaa caaccaggct gcaaccagaa aggcgatggc tacaaaaaag
     4381 ccgaatgaga aaatgggaag attgatatgt gctccggtca ggtggctgat gagttcacca
     4441 agagtggcat aggccctgag tattgtcata ctgatttgct tacgttgttt aaaatgagag
     4501 attcatcaga aaccggtacc ggagtctggc attcgaactg ggtatccggc agcagttcat
     4561 cgagaccaca ccaggcaagt tcattgacca gaagcgggtc aaaggctgtt ctgaccctga
     4621 tatagttatc agtaaatccc gtcatccata tgccgtcagt ttctgcttcc agcaggactt
     4681 ttctcattgt gccggaatat ttctgataga atgcccgctt ctttttttct gatagtgaga
     4741 tcagttgtgt ggtgcgctcc tgcctcactg ctccgggtac cggatctgcc attttaatgg
     4801 cacgggtatc aggtctttca ctataggtga acacatgcag atagctcaca tccagctcct
     4861 gcagaaagtg ataagtttcc agaaagtcag catgagactc gccgggaaat ccggtgatca
     4921 catcaacacc gatacctgca tcgggcatca gatcacgtat cagttgaata cgatgtgcat
     4981 acagatcttt tttgtagcgg cgacccattg ctttcaggat cttgtcgctg ccgctttgca
     5041 gaggaatgtg aaagtgcgga acaaatctct ttgacccggc tacaaaccga atgatatcat
     5101 cagacaacag gttgggctca atggaagaga tcctgaatcg ttcaatgcct ttcagctcat
     5161 ccagtgtctg aataagttga agaaatgttt cccggtcttc cggcaatact ccttcccttc
     5221 tgccaaagtc gccaatattg acccctgtta aaacgatctc tttcacgcca tttgcagctg
     5281 catcttctgc ctgcctgcgc acctgttcaa tagtgccgct tctgctcttt cctctggcca
     5341 ggggtatcgt gcagaatgcg cactggtagt cgcatccatc ctgtaccttg agaaaagtgc
     5401 gtgtgcgatc ctgaaatgag taggcaggca agaaatcctt tactacatct atggggcccg
     5461 ctatcacact ggtttctgta gtatctgtca ggttgttgag gtgttgggga aggttgaact
     5521 tttctgctgc acccaatacc agatcaacac catctatctc agcaatttcc tttggtttca
     5581 gttgcgcata acacccgacg atggccactc tggcatccgg acgaaacttt tttgcctgtc
     5641 tgaccacttt tctgcatttc ttatctgcat gatcggtcac actgcaggta ttgatcacat
     5701 atacgtctgc accctgctgg aatggcacca gcctgtatcc tgcttcttcc atttgtctgg
     5761 acagggagga agattcagaa aagttgagct tacaccccag tgtatagaat gaaaccgtgc
     5821 gctgcatggt actgcaaagt tacgaaacca cggccgggtg cctaactggc caaagtcgta
     5881 atttcgggcc atgggaatgt tggcccgcat aggagcttat gaatacgatc tggaacaggg
     5941 ttttgctatc tgtatccctt ttcatcaggg gcctgatcag gtcaattgct attatgcacc
     6001 accattttca gtttcgcctg tagtgatggg cgactttaca ggagagatca gttcaggcgc
     6061 acctgtcaac taccgcaaca tttctgtcaa tccacatgga aacggcacgc ataccgaatg
     6121 cctggctcat atttcagatg cagacctgga tgtccatgca gcgctcactg attccttttt
     6181 tccggttcag gtgctgacca ttgctccgca tcgtcttaca aacggcgatc aggttcttcg
     6241 tttcagagaa gcagaaattc ttcctgaaat aaaagcgctt gccatacgct ccctgcccaa
     6301 tacatcagac aagcaacaca gagcctattc cggcaccaat ccgccttatc tggatgccgc
     6361 ttgtgtggct ttgctgcctg acctgggtat tgatcatctg ctcattgatc tgccttcggt
     6421 tgacaaggag gaagatggtg gtgccctggc tgcccacaaa gctttctgga attttgcttc
     6481 cgttaaaaga acgcatgcaa ccataacaga actgatattc attcctgatc acatcaaaga
     6541 cggccggtat ctcctgaatc tgcagatagc tccattcaga acagatgcca g
//
LOCUS       DAOMAX010000142         6573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1851_length_6573_cov_4.912550, whole genome shotgun sequence.
ACCESSION   DAOMAX010000142 DAOMAX010000000
VERSION     DAOMAX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_6573_cov_4.912550"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PK742_08215"
     CDS             complement(<1..1638)
                     /locus_tag="PK742_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQU76617.1"
                     /translation="MSKESTIIKEFGLSTFAVKNRISVMFLALIILIMGVSAYNAMPR
                     ESFPEITQPEIYIGVLYPGNSPKDVENLITRPIEKELNTLTDVDEIRSTSIQDYATIT
                     VAFNYDVDVDAALQDVKDAVDKAKGDLPNDLDNEPNIFALDFSEFPVMNINLYGIDDV
                     DLLKDYAEYLEDEIEKVEAVSGVDIRGVLDKEVKVSIDKERMETLEISFYDIESAISA
                     ENLTMSAGEILTDGARRDIRIVGEFSDVSQLENVIVKYENQRIVYLRDIADVSFDYIE
                     PNSYARLDEENVITLDVKKKSGGNLIQASEKIQEIIKDAEDSGRFPPELKIIITNDQS
                     RDTKLMVDSLENNIISGVILVVLVLLFFLGLRNASFVGIAIPLSMLMAFMILNAFGIT
                     LNMMVLFSLILALGMLVDNGIVVVENTYRLMSEGKNPIRAAIEGVGEVAWPIIASTAT
                     TVAAFIPLLFWNNLMGEFMKYLPLTLIITLSSSLFVGLVINPVIAARFMKVQEEGTTA
                     KKPSRNFWYLVAGALIIGVLAHLARATFLGNLAIVVAL"
     gene            complement(1640..2797)
                     /locus_tag="PK742_08220"
     CDS             complement(1640..2797)
                     /locus_tag="PK742_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQU76618.1"
                     /translation="MKRTIAIIPVIAILLSACGAKEEVTDSVEAKMAQIAEYKSEIKD
                     LEIKIRDLEKEIIANGGSLELPPDTVAVTTMTIERGTYEEFVEVAGSVSSDQNILVSS
                     EMGGTVTRIYVKEGQQVSAGQLLLSTDDQVLQKSIDELQNAYDLAKTVYEKRKALWDQ
                     KIGSEIEYLTAKNNMESLELKLNTTRTQLAKTQLKSPISGTVDEIITKTGEMASPGMP
                     LLRVVNLSEVQIECDISEAYLGRVKRGDKVNVSFPSINFESVATITNVGQVINPSNRT
                     FKLQVSLNNRDGMLKPNLLGYLQIREYVEQDQVIIPTRLIQNGVNSDFVYAITDGSVV
                     KIDITRGRSHNGFTQIKSGLSGGEILVVEGNRQVKEGDIARVVGGETASKE"
     gene            complement(2808..4130)
                     /locus_tag="PK742_08225"
     CDS             complement(2808..4130)
                     /locus_tag="PK742_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQU76619.1"
                     /translation="MRSNLMIWFLLTGALSASAQEMSLSLDEARAYAVQHNTQMQTSQ
                     LNIADGRTTLQSRTSVGLPQLSASVGYNHFITLPTSLIPAEFFGGEPGDFAEVQFGTP
                     ENMTAGISASQLLFSGPYIYGVQAAKVYVELLQQQDKVTANDVKKNVELAYYTVLIAQ
                     KMAQIAGENLEVVNQTLHEVSEMNKAGFVEEIDVNRLEMTSANLSNILANASRQEVFA
                     KNLLKYQMGMDLSQEIVLTDSLEQLEQGLVLLQNDQNFLNRPEFQVMETTKRLNELNV
                     QVNKSSYLPTLAVFGSYEQAAQRNEFNFFDGNEPWFETFLVGVQLNVPIFSGMDKKAA
                     IESAQIGYDKAVVAEQTLKQSVQLEIAQYTTDYLNAKDDLATQQSTIELANKIFNTAY
                     VKYREGLGSSLELTQAQGDLLAAQANYINALYSLLNAQTNLKKALGYL"
     gene            complement(4120..4743)
                     /locus_tag="PK742_08230"
     CDS             complement(4120..4743)
                     /locus_tag="PK742_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQU76620.1"
                     /translation="MAELSEEIKILERCVRLFMRYGVKSVTMDDIARELGMSKKTLYQ
                     YFSNKNDLVNKVARLHFEAENEAIHTICTTSANAIDELLNISRWVSTQMKGINPTLML
                     DLQKYHPDAWAVFQSHKNSDVYQCMLQNIHRGMEEGLYRRDINPEIIARLYIGKMELV
                     VDTELFPPGEFSFQHIHHEFIHYHIRGIASERGLAYLEQIQNQPYAQ"
     gene            complement(4938..6089)
                     /locus_tag="PK742_08235"
     CDS             complement(4938..6089)
                     /locus_tag="PK742_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQU76621.1"
                     /translation="MRFLSATTIHPVSSPALSGYVLVIHDDGTIEDLLPESVVDPTHV
                     EFFEGDLIPGFVNAHCHLELSHLRGSFPEKTGLPDFLNKVVSQRQADPDAVAEAMRFA
                     DSEMWENGIMAVGDICNKSDSFSVKADSRIHYHSFVELLAFDPDRAVKALAEGNSLLE
                     QLNQFGLTGSLVPHAPYSVSPALVFLITRQCADAGLPTSIHMLESNDETEFFVQGTGL
                     YRRLYHDMGMAIDFFEPSGKTSLETLLPWFHPMAKTLLVHNTIATEQDVFSAVSQHTN
                     LWWCFCPNANLYIEDRLPDMPQLLRTMPPGRITLGTDSLASNHQLSIWQEMLAIMEAF
                     PEISLETILPWATLNGARFLGLEQTLGSLETGKRPGILHLHQDTIGRIC"
     gene            complement(6171..>6573)
                     /locus_tag="PK742_08240"
     CDS             complement(6171..>6573)
                     /locus_tag="PK742_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HQU76622.1"
                     /translation="DAKGFIAFIPWTFQDVDTTLKKIRRAKNDTTPEEYIRMISLSRI
                     MLPNIDNIQASWLTVGKQVAQLCLHAGANDFGSIMIEENVVSAAGAPHRFTYKTIQDA
                     IREAGFEPQLRNQQYEDRELPEGIEEQVIAY"
BASE COUNT         1477 a         1654 c         1704 g         1738 t
ORIGIN      
        1 gagtgctact acaatagcca ggttacccag gaaggtggct ctggccagat gtgccagcac
       61 tccgatgatc agcgcacccg ccacgaggta ccagaaattc ctggaaggtt ttttcgcagt
      121 ggtaccctct tcctgcacct tcatgaagcg tgctgcgatg accgggttga tgaccaggcc
      181 gacgaacagc gatgaagaca gggtgatgat gagggtgagc ggcaggtatt tcatgaactc
      241 tcccatcagg ttattccaga acaggagggg gatgaaggca gccacggtag tagcagtgga
      301 ggcgatgatg ggccaggcca cttcgcccac gccttcgatg gctgcacgga tcggattctt
      361 gccttcactc atcagacgat aggtattctc taccaccacg ataccattgt ccaccagcat
      421 acccagcgcc aggatgaggc tgaataacac catcatgttc agggtgatac caaaggcgtt
      481 caggatcatg aaggccatga gcatggacag tggaatggcg atacccacga aggaggcgtt
      541 ccgcaatccg aggaagaaga gcagtaccag caccaccagg atgacaccgg atatgatgtt
      601 gttctccagg ctgtccacca tcagtttggt atcacggctc tggtcgttgg tgatgatgat
      661 cttcagctcc ggcggaaagc ggccgctgtc ttcggcatcc ttgatgatct cctgtatctt
      721 ttcagatgcc tggatcaggt tgccaccgct tttctttttc acgtcaaggg tgatgacatt
      781 ctcttcatcg agacgggcat aggagttggg ttcgatgtaa tcgaaagaaa cgtcagcaat
      841 atcacgcaga taaacgatgc gctggttctc gtatttgacg atgacatttt ccagctgcga
      901 tacatcgctg aattcaccca cgatgcggat gtcacggcgg gcaccatcgg tcaggatctc
      961 gcccgcactc atggtgaggt tttcggcaga tatggcactc tctatatcat agaaactgat
     1021 ctcgagggtt tccatccgct ccttgtcgat gctcaccttc acttccttat caagcacacc
     1081 acgaatatcc acgccggata ctgcttctac cttttctatc tcatcttcca ggtattcggc
     1141 ataatctttc agcaggtcca cgtcatcgat gccatagagg ttgatgttca tgaccgggaa
     1201 ttctgaaaaa tccagggcga aaatgttggg ttcattgtcg aggtcgttgg gcagatcgcc
     1261 tttggcttta tcgacagcat ctttcacatc ctgcagggcg gcatctacat ccacatcgta
     1321 gttgaatgcc acggtgatgg tggcataatc ctggatggaa gtggaacgga tctcatctac
     1381 atctgtcagg gtattcagtt ccttttcgat aggacgggtg atgaggttct ctacatcctt
     1441 gggagagtta cccggataga gcacaccgat atagatctct ggttgggtga tctccggaaa
     1501 actttcacgg ggcatggcgt tgtaggcgct cacccccatg atgaggatga tgagcgccag
     1561 gaacatgacg ctgatgcggt tcttcaccgc aaaagttgac agcccgaatt cttttatgat
     1621 ggtactttcc ttgctcatgt tattcctttg atgcggtttc tccgcccact acacgggcaa
     1681 tatctccttc cttcacctgg cggttgcctt ccactaccag aatttcacct ccactgagac
     1741 ctgatttgat ctgcgtgaat ccgttgtgtg aacggccacg tgtgatatcg atctttacca
     1801 cagagccatc agtgattgca tatacgaaat cactgttcac accattctgt atcagacggg
     1861 taggaatgat cacctgatcc tgctctacat attcgcggat ctgcagataa cccagcaggt
     1921 ttggtttcag catgccatcg cggttgttca ggctcacctg cagtttgaag gttctgttgg
     1981 atgggttgat gacctgacct acgttggtga tagtagccac tgattcgaag ttgatggagg
     2041 ggaagctcac gttcacttta tcgccacgct tcacacgacc cagataggct tcactgatat
     2101 cacattcgat ctgtacttca ctgaggttga ccacccgcag caagggcata ccgggcgatg
     2161 ccatttcgcc ggtcttggtg atgatctcat caaccgttcc gctgattgga gatttcaatt
     2221 gggtcttggc cagctgggtc ctggtggtgt tcagtttcag ttccaggctt tccatattgt
     2281 tcttcgccgt caggtattcg atctctgaac cgatcttctg atcccacagg gccttgcgct
     2341 tttcatacac ggtctttgcc agatcgtagg cattctgcag ttcgtctatg cttttctgca
     2401 acacctgatc atctgtgcta agcagcagtt gcccggcgct tacctgctgt ccttccttca
     2461 catagatgcg ggtcacggtg ccacccattt cgctgctcac cagaatgttc tggtcagagc
     2521 tgacggagcc ggccacttct acgaattctt cataagtacc gcgttcaatg gtcatggtgg
     2581 tgaccgccac ggtatcaggc ggaagctcca ggctgccacc atttgcgatg atctcctttt
     2641 ccagatcacg gatcttgatc tccaggtcct tgatctctga tttgtattcc gctatctgtg
     2701 ccattttggc ttctacggaa tcggtcactt cttctttggc accacaggcg ctgagcagaa
     2761 tggcgatgac cggaatgatt gcgatagtcc ttttcatatg caggttttta cagatagccg
     2821 agggctttct ttaggttggt ttgtgcgttg agtaatgaat atagtgcgtt gatatagttg
     2881 gcttgtgctg ccagcaggtc gccttgtgcc tgggtcagct ccaggctgga acccagccct
     2941 tcgcggtatt tgacatatgc ggtgttaaag atcttgttgg ccagttcaat ggttgactgc
     3001 tgggtagcca gatcatcctt tgcattcagg taatcagtag tgtactgggc aatttccagt
     3061 tgcacggatt gttttagcgt ttgttcggca accacagctt tgtcataccc gatctgtgca
     3121 ctttcaatgg ctgctttctt atccatgccg gagaagatgg gcacattgag ctgcacgcct
     3181 accagaaagg tttcgaacca gggttcattg ccatcgaaga aattgaattc gtttctctgg
     3241 gcagcctgtt cataggaacc gaatacggcc agagtgggca ggtaagaaga cttgttcacc
     3301 tgcacattga gctcattcag tcgcttggtg gtttccatga cctggaactc cggacggttc
     3361 aggaaattct ggtcgttttg cagcagtacc agtccctgtt ccagttgctc cagtgaatcg
     3421 gtcagtacga tctcctggct gaggtccata cccatctgat acttcagcag gtttttcgca
     3481 aagacctcct gtctgctggc attggccagt atgttactga ggttggcact ggtcatttcc
     3541 aggcggttca catcgatctc ttccacgaat ccggccttgt tcatttcaga cacttcatgc
     3601 aatgtctgat tgaccacctc caggttttcg cctgcgatct gcgccatctt ctgcgcaatg
     3661 agtactgtgt aataggccag ctccacgttc ttcttcacat cattggccgt aaccttatcc
     3721 tgctgttgca acagttccac atagactttg gccgcctgca ccccatagat gtaaggaccg
     3781 ctgaagagca actgcgaagc agagataccg gcggtcatgt tctcaggtgt accgaactgc
     3841 acctctgcaa agtcgcccgg ttcgccgcca aagaattcag ccgggatgag gctggtggga
     3901 agggtaatga aatggttgta acccaccgat gcactcaatt gcggaagccc tacagaagtg
     3961 cggctttgca aggtggttct gccatcggca atgttcaact ggctggtttg catctgggta
     4021 ttgtgctgca cggcatatgc tctagcttcg tccagactca gggacatttc ctgggcactg
     4081 gcagataagg cgccggtcag caggaaccag atcatcaggt tactgcgcat agggttggtt
     4141 ttgtatttgt tcgagatagg caagaccgcg ttcgctggcg atgcctctga tatggtaatg
     4201 aatgaattcg tggtggatgt gctggaagct gaattcgccc ggagggaaaa gttccgtgtc
     4261 aaccaccagt tccattttgc cgatatagag ccgggcaatg atctccgggt tgatgtcgcg
     4321 gcgatacagg ccttcttcca ttccacgatg gatgttctgc agcatacatt gatagacatc
     4381 gctgttctta tggctctgaa agacggccca ggcatccgga tggtatttct gcaggtccag
     4441 catcagggtg gggttgatgc ccttcatctg ggtgctgacc catcgggaga tgttcagcaa
     4501 ttcatcaatg gcgttggcac tggtggtgca aatggtatga atggcctcgt tctcagcttc
     4561 gaaatgcaaa cgggccactt tgttcaccag gtcatttttg ttggagaagt actggtacag
     4621 ggtctttttg gacataccca gctcgcgggc aatatcgtcc atggtcacgc tcttcacgcc
     4681 ataccgcatg aacagacgta cacaacgctc cagtattttg atctcttcgc tcaattctgc
     4741 cattttccgg cttttcgggc cgcaaatgta cagacgaaaa caggggaaac aaattttagt
     4801 ttaaaagttt ccaaagtttt ttaattattt aacacgccac catttttcgt ccgatctgat
     4861 aaaggtgaaa agcagacctt acagcaaaaa aagggatcga cgaaaaggaa tattatcgtg
     4921 atgaacgggc cgaaagatca gcaaatcctg ccgatcgtat cctgatgcag gtgcaggata
     4981 cctggccgct taccggtttc cagagacccc agggtttgtt ccagtccgag aaaacgtgca
     5041 ccgttcaaag tagcccaggg caatatggtc tccagtgaga tttcagggaa tgcctccatg
     5101 atggctagca tttcctgcca gatgctgagc tggtggttgg atgccaggct gtcggtgccc
     5161 agggtgatgc ggccgggagg catggtgcgc agcagttggg gcatgtctgg cagccgatct
     5221 tctatataca gattggcgtt cggacaaaaa caccaccaca ggttggtgtg ctgcgaaacg
     5281 gctgagaaca catcctgctc cgtggcgatg gtattgtgca ccagcagggt cttcgccatc
     5341 gggtggaacc agggcaacag ggtttccagt gaggtcttcc ccgaaggttc aaagaaatcg
     5401 atggccatgc ccatatcatg atacaacctg cggtacagtc cggtaccctg cacaaagaac
     5461 tcggtctcat cattgctctc cagcatgtgg atggacgtag gcaggccggc atctgcacat
     5521 tgacgggtga tgagaaatac gagggccgga ctgaccgaat aaggcgcatg cggcaccagg
     5581 cttccggtca gaccgaactg atttaattgc tctaacaacg aattgccttc tgctaatgct
     5641 ttgacagccc ggtccggatc gaatgccaat aactccacga agctgtgata gtgtatccgg
     5701 ctatcagctt tcacagaaaa ggaatccgac ttgttgcaga tatcgcccac ggccatgatg
     5761 ccgttctccc acatttcgct gtcggcaaag cgcatggctt cagctactgc atccggatct
     5821 gcttgccgct gactcaccac cttgttcagg aaatcgggta gtccggtctt ttcaggaaaa
     5881 cttccacgca ggtgcgacaa ctccagatgg caatgtgcat tgacgaaacc agggatcaga
     5941 tcgccttcaa agaattcgac atgagttggg tcgacaactg attccggtaa aagatcttct
     6001 atggtgccat catcatggat caccagtaca tagccgctca gtgcaggaga ggatacgggg
     6061 tggatggtgg tggcagagag gaaacgcatt tcaaaaatgt gaagatgtgc aaatgtgaaa
     6121 atgatcagat tgttggagta tcacattttc acatttgcaa atttagacat tcaataagcg
     6181 atcacctgct cttcaatacc ttccggtaac tcccggtctt cgtattgctg gttgcgaagc
     6241 tgcggttcaa agcctgcttc acggatggcg tcctggatgg ttttgtaagt gaagcgatgc
     6301 ggagcaccgg cggcactcac cacattctct tcgatcatga tgctgccgaa gtcattggca
     6361 ccggcatgca agcacaattg tgctacctgc ttcccgaccg tgagccagct tgcctggata
     6421 ttgtcgatgt tgggcagcat aatacggctc agggaaatca tgcggatgta ttcttctggt
     6481 gtggtatcgt tcttggccct tctgatcttc ttcaatgtgg tatcgacatc ctggaaggtc
     6541 cacggtatga atgcgatgaa acccttcgca tct
//
LOCUS       DAOMAX010000143         6567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1852_length_6567_cov_4.937346, whole genome shotgun sequence.
ACCESSION   DAOMAX010000143 DAOMAX010000000
VERSION     DAOMAX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1852_length_6567_cov_4.937346"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..222
                     /locus_tag="PK742_08245"
     tRNA            150..222
                     /locus_tag="PK742_08245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:183..185,aa:Thr,seq:ggt)
     gene            318..1505
                     /gene="tuf"
                     /locus_tag="PK742_08250"
     CDS             318..1505
                     /gene="tuf"
                     /locus_tag="PK742_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HQU76623.1"
                     /translation="MAKEQFVRSKPHVNIGTIGHVDHGKTTLTSAITMVLADMGLAQK
                     KDYDSIDNAPEEKERGITINTAHVEYETENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPAVVVFMNKVDLVDDPELLELVEMEVRDLLSF
                     YDFPGDTISIIKGSALKALEGDAEGRQQILDLMAAVDADIPVPPRETEKPFLMPVEDV
                     FSITGRGTVATGRIERGVINSGEAVDIIGLQTETQKSTVTGVEMFRKILDRGEAGDNV
                     GLLLRGIEKDQIKRGMVICKPASVTPHTEFKCEVYVLKKDEGGRHTPFFNKYRPQFYF
                     RTTDVTGEVQLPDGVEMVMPGDNIGFVVKLIYPIAMEKGLRFAIREGGRTVGAGQVTE
                     IIK"
     gene            1548..1620
                     /locus_tag="PK742_08255"
     tRNA            1548..1620
                     /locus_tag="PK742_08255"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1581..1583,aa:Trp,seq:cca)
     gene            1638..1829
                     /gene="secE"
                     /locus_tag="PK742_08260"
     CDS             1638..1829
                     /gene="secE"
                     /locus_tag="PK742_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQU76624.1"
                     /translation="MNRIKAFILEAYQELRYKVSWPTWSELQSSTSVVFIAIAILSVM
                     VFVMDFASSRLLDLFYGLF"
     gene            1848..2396
                     /gene="nusG"
                     /locus_tag="PK742_08265"
     CDS             1848..2396
                     /gene="nusG"
                     /locus_tag="PK742_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQU76625.1"
                     /translation="MSDGNKWYVLRVISGKERKLKEYLDQHIKRLEWDTIVSQVLVPT
                     EKVYKVKNGKKVIQEKNSLPGYILIEAAEGKLTPEMIQSITGITNVIDFLGKANPIPL
                     RRNEVNRILGKMDEMNAEGETLNEPFIIGETVKIIDGPFNDFTGNVDEVFEDKKKVKV
                     IVKIFGRKTPVELNFMQVEKVN"
     gene            2398..2841
                     /gene="rplK"
                     /locus_tag="PK742_08270"
     CDS             2398..2841
                     /gene="rplK"
                     /locus_tag="PK742_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQU76626.1"
                     /translation="MAKEIQTYIKLQIKGGQANPAPPVGPALGAKGVNIMDFCKQFNA
                     RTQDAQGKVLPVVITVYKDKSFDFIIKTPPAAVLLMEAAKKKKGSAESNRIKAGSVSW
                     DQVRKIAEEKMPDLNCFTIESAMKMVAGTARSMGFSVEGAAPWDN"
     gene            2868..3563
                     /gene="rplA"
                     /locus_tag="PK742_08275"
     CDS             2868..3563
                     /gene="rplA"
                     /locus_tag="PK742_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQU76627.1"
                     /translation="MRLTKNRKKAEGLVEQNKLYSIEEAAQLVKQTNTAKFNASVDLH
                     IRLGIDPRKADQAIRGTTALPHGTGKDKSVLVLCTPDKEDEAKAAGADFVGLDDYVEK
                     IMGGWTDVDVVIATPAVMPKIAKLGRVLGPRNLMPNPKVGTVTNEVGNAVTEVKKGKV
                     AFKVDKFGIIHSSIGRVSFDPAQIAENSEELIRTLARMKPSSAKGTYFKSITMASTMS
                     PGIAIDPKTVSGI"
     gene            3582..4112
                     /gene="rplJ"
                     /locus_tag="PK742_08280"
     CDS             3582..4112
                     /gene="rplJ"
                     /locus_tag="PK742_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQU76628.1"
                     /translation="MNKEQKTQAIAELTETVAQYDSLYITDASTLTVADVNKLRRLCF
                     EKNIHFEVVKNTLLRKALENHGEKYEGLMGLLEGPTSVMFCEVAVEPAKVISKFRKEA
                     DKGKPELKGAYIGEAVFIGDDQLEVIAKMKSKEELIGDIIGLLQSPAKNVISALQSSG
                     GKLAGILKTLSEKEEA"
     gene            4158..4541
                     /gene="rplL"
                     /locus_tag="PK742_08285"
     CDS             4158..4541
                     /gene="rplL"
                     /locus_tag="PK742_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQU76629.1"
                     /translation="MSELKEFAEKLVSLSVKEVNELAQILKDEYGIEPAAAAPVMVAG
                     GGGAAGGGEAAAEKTSFDVILTSAGAAKLSVVKVVKDVTGLGLKEAKELVDGAPKPVK
                     EGVSKDEAESLKAALVEAGAEVEIK"
     gene            4656..>6567
                     /gene="rpoB"
                     /locus_tag="PK742_08290"
     CDS             4656..>6567
                     /gene="rpoB"
                     /locus_tag="PK742_08290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQU76630.1"
                     /translation="MPAKSSKPTERINFGKIRKATDYPDLLDIQVQSFKDFFQLETTQ
                     ENRSNEGLYKVFMENFPITDARNIFLLEFLDYFVDPPRYSMDECMERGLTYSVPLKAK
                     LRLSCNDEEHIDFQTIVQDVFLGNIPYMTPKGTFIINGAERIVVSQLHRSPGVFFGQS
                     IHPNGSKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRAIGYDTDKDI
                     LELFGLAEELKADAKTLKKNIGRKLAARVLKTWREDFVDEDTGEVVSIERNEVILERD
                     TILTEENIDHILETGVSHVILTLDEVVSDYTIILNTLQKDVSNSELEAVQQIYRQLRG
                     AEPPDDETARGIIDKLFFSDKRYDLGEVGRYKINEKLGMETSLDTKVLTKADIIAIVK
                     YLVELSNNKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFT
                     PIELINARTLSSVINSFFGTSQLSQFLDQVNPLAEITHKRRISALGPGGLSRERAGFE
                     VRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINKMGFIETPYRKVVKGKVEAKGE
                     VVYLNAEEEDHMVIAQANAKVDDKGNFINDRVKCREQGDFPEYEPDRIQYMDVAPNQI
                     VGVSASMIPFLEH"
BASE COUNT         1831 a         1571 c         1697 g         1468 t
ORIGIN      
        1 tagagcatca gccttccaag ctgagggtcg cgggttcgag tctcgtctcc cgctcgccga
       61 aacaaacgga tcagcttcca ggatccggtt tgtagtgccg ccggaaggcg gagaacatat
      121 tttcacccga gcgtcagcaa cggtgaactg ccaatgtagc tcagttggta gagcacatcc
      181 atggtaagga tgaggtcacc agttcgatcc tggtcattgg ctctgtgtcg tcagacagaa
      241 catctgcagt ggcagggttc tgttgagcgg ctgtaaatag agttgagttt tttaaaccta
      301 aataaacact atacaaaatg gctaaagagc aatttgtacg tagcaaaccc cacgtgaaca
      361 tcggaactat cggtcacgtg gatcacggta agaccaccct tacctctgct attaccatgg
      421 tgctggccga catgggtctg gcacagaaga aagattatga ctcgatcgat aatgctccag
      481 aagaaaaaga aaggggtatt acgatcaaca cagcacacgt tgagtacgaa actgaaaacc
      541 gtcactatgc acacgtggac tgtccgggtc acgccgacta tgtgaagaac atggttacag
      601 gtgctgctca gatggacggt gccattctgg tggtagctgc tacagatggt ccgatgccgc
      661 aaacccgtga gcacatcctc ctggctcgtc aggtaggtgt tcccgcagta gtggtgttta
      721 tgaacaaagt ggatctggtt gacgatcctg aactgctcga actggtggaa atggaagttc
      781 gcgacctgct gagcttctat gacttccccg gcgataccat ctctattatc aaaggttctg
      841 ctctgaaagc actggaaggt gatgcagaag gacgtcagca gatcctggat ctgatggctg
      901 cagtggatgc cgatattccg gtacctcctc gtgaaactga aaagccgttc ctgatgccgg
      961 tagaagatgt attctctatc actggtcgtg gtactgtggc tactggtcgt atcgaaagag
     1021 gtgtgatcaa ctccggtgag gctgttgata tcatcggtct gcagacagaa actcagaaat
     1081 ctaccgtaac aggtgtggaa atgttccgca agatcctgga tcgtggtgaa gccggcgaca
     1141 acgttggtct gctgcttcgc ggtattgaaa aagaccagat caaacgtggt atggttatct
     1201 gtaaacctgc ttctgttacc ccgcacactg agttcaagtg tgaagtgtac gttctgaaga
     1261 aggatgaagg tggtcgtcac actccgttct ttaacaaata ccgtccgcag ttctatttcc
     1321 gtaccactga cgtaactggt gaagtgcaac ttcctgatgg cgtggaaatg gttatgcccg
     1381 gcgataacat cggtttcgtt gttaaactca tctacccgat cgctatggaa aaaggtctgc
     1441 gtttcgctat ccgcgaaggt ggccgtaccg ttggtgccgg tcaggtaact gagatcatca
     1501 agtaatcatc attgtgtatc cgggggaact tcggttcccc cctatacacg ggtgtagctc
     1561 aattggtaga gtggcggtct ccaaaaccgc aggctgggag ttcgagcctc tccacccgtg
     1621 ccggaaaaga aaaaactatg aatagaatca aggcattcat actggaagca taccaggaac
     1681 tgcgttacaa ggttagctgg cctacctggt cggaactgca aagcagtacc tccgtcgtgt
     1741 tcatcgccat tgccatactg tctgtgatgg tatttgtgat ggatttcgcc tccagcagac
     1801 tccttgatct gttctacggt ttgttctgaa cccagtaaag gaaagagatg agcgacggta
     1861 ataagtggta cgtactgagg gtcatcagcg gcaaggaacg caagctgaaa gaatacctcg
     1921 accagcatat caaaaggctg gagtgggata cgatcgtctc gcaggtactg gtgcctacag
     1981 aaaaggtcta taaagttaaa aacggcaaga aagtgatcca ggaaaaaaat tccctgccgg
     2041 gatatatcct gatcgaagct gccgaaggaa aactgacccc ggaaatgatc cagtctatca
     2101 ccggtatcac caatgtgatc gatttcctcg ggaaagccaa tcctattcca ctcagaagaa
     2161 atgaggtgaa caggatcctg ggtaaaatgg atgaaatgaa tgccgaaggg gagaccctta
     2221 atgagccatt catcatagga gaaacggtga agatcataga tggtccgttc aacgatttca
     2281 ccggtaatgt ggatgaagtc tttgaagaca agaagaaggt caaagtgatt gttaaaatat
     2341 ttggcagaaa gactccggtg gagctcaatt tcatgcaagt agaaaaagtc aactaagatg
     2401 gcaaaggaaa ttcaaaccta catcaaactg cagatcaaag gcggccaggc taatccggct
     2461 cctccggtgg gcccggcact cggtgccaag ggcgtgaaca tcatggactt ctgcaaacag
     2521 ttcaatgccc gtactcagga tgcacagggt aaggtgctgc cggtggtgat caccgtgtac
     2581 aaagacaagt catttgactt catcattaaa acccctcctg cagctgtgtt gctgatggaa
     2641 gcggccaaaa agaagaaggg cagtgcagaa tccaaccgta tcaaggctgg ttccgttagc
     2701 tgggatcagg tgcgcaagat cgctgaagaa aaaatgcctg accttaattg ctttaccatc
     2761 gaaagcgcta tgaaaatggt ggccggaacc gcccgcagta tgggcttttc tgtagaaggt
     2821 gcagcaccct gggataacta attcgatcag attcaaaatc atatacagtg agattgacca
     2881 agaacagaaa aaaggcggaa ggcctggttg aacagaataa actgtactct attgaagagg
     2941 cagctcagct cgtaaagcag accaatactg ccaagttcaa cgccagcgtt gacctgcata
     3001 tccgcctcgg tatcgacccg cgtaaagccg atcaggccat ccgtggtact accgctctgc
     3061 cacatggaac cggtaaagac aaaagtgtac tggtgctctg tactccagac aaggaagatg
     3121 aagcaaaagc ggccggtgca gacttcgtag gtctggatga ctatgtggag aagatcatgg
     3181 gcggctggac agatgtggat gtggtcattg ctacacctgc tgtcatgccc aagatcgcca
     3241 aactgggtcg cgtacttggt ccgcgtaacc tgatgccaaa cccgaaagtt ggaaccgtta
     3301 ccaacgaggt aggcaacgca gttaccgaag taaagaaagg taaagtggcc ttcaaggttg
     3361 acaagttcgg aatcatccac tcctccatcg gacgggtgtc atttgaccct gcccagattg
     3421 ctgagaacag cgaagaactg atccgcaccc tggcccgaat gaagccatcg tctgcaaaag
     3481 gaacctattt caaaagcatc accatggcct ctaccatgag ccccggtatt gccatcgacc
     3541 cgaaaacagt atccggtata taacatcgaa accacgagac aatgaataag gaacaaaaga
     3601 cacaggccat tgctgaactg accgaaacag tcgctcagta tgacagcctg tatatcacag
     3661 atgcctccac cctgaccgtg gcggatgtga acaaactgcg tcgcctgtgc ttcgagaaga
     3721 atattcactt cgaagtggtc aagaataccc tgctgcgcaa agcactggaa aaccacggcg
     3781 aaaagtatga aggactgatg ggactgctgg aaggccctac ctctgtcatg ttttgtgaag
     3841 tagctgtaga acctgccaag gtcatcagca aattccgcaa agaagctgac aagggtaagc
     3901 cagaactgaa aggcgcctac attggtgaag cagtatttat cggtgatgac cagctggaag
     3961 tgatcgcgaa gatgaagtct aaagaagaac tcatcggcga tatcatcggt ctgctgcagt
     4021 ctcctgccaa gaatgtcatt tctgccctcc agtcatccgg tggaaaactg gcaggtatcc
     4081 tgaagaccct gagtgagaaa gaagaagcct gaaatattaa tccataatca tattcacaaa
     4141 tccataaacc aatagcaatg tcagaattaa aagagtttgc agaaaagctg gttagtttgt
     4201 ctgtaaaaga ggttaacgag ttagcccaga tcctcaaaga cgagtacggc atcgagcctg
     4261 ctgcagctgc tcctgtaatg gtagccggtg gcggtggtgc tgctggtggt ggcgaagctg
     4321 ctgccgagaa gacatctttc gatgttatcc tgaccagcgc aggtgctgcc aaactgtctg
     4381 tggtgaaggt ggtgaaagac gttaccggtc ttggcctgaa agaagccaaa gaactggttg
     4441 acggcgctcc gaagccggtg aaagagggtg ttagcaaaga tgaggcagaa agcctgaagg
     4501 ctgccctcgt tgaagccggc gctgaagtcg agattaaata ataagtgatc taccccggga
     4561 tctttcatga tcccgggccg tcactcaaag gttataccgc cagaggcggt atgccttatt
     4621 gttatttata tcttactaaa cttcatcaac cggatatgcc agcgaaatca tccaagccaa
     4681 cagaaaggat caattttggt aaaatacgta aagcaactga ttacccggat ctgctagata
     4741 ttcaggtcca gtcctttaag gacttctttc agctggaaac gacccaggag aaccgcagca
     4801 atgaagggct gtacaaggtg ttcatggaaa acttcccgat caccgatgcc cgcaacatct
     4861 tcctgctgga attcctggac tatttcgtcg atccgccccg ttactcgatg gacgagtgta
     4921 tggagcgtgg actgacctat agcgttccat tgaaagccaa gttgcgcctg tcctgtaacg
     4981 acgaagaaca catcgatttc cagaccattg ttcaggatgt tttcctgggc aacattccgt
     5041 atatgacccc gaaagggaca tttatcataa acggagcaga aaggatcgtc gtgtctcagt
     5101 tgcaccgctc acccggtgta ttcttcggac agagtattca tccaaacgga tccaagatct
     5161 attcagccag ggtcatcccg ttcaagggtg cctggatgga atttgccact gacatcaata
     5221 acgtgatgta tgcctatatc gaccggaaga agaaattccc ggttacaacg ttattgcgtg
     5281 ccataggcta tgacaccgat aaggatatcc tcgaattgtt cggcctggcc gaagaactca
     5341 aggcagatgc caagaccctg aagaagaaca tcggaaggaa actggccgca cgggtcctca
     5401 agacctggag agaggatttc gttgacgaag ataccggaga ggtagtgtcc atcgagcgga
     5461 acgaagtgat cctcgaaaga gataccatcc tcactgaaga gaatatagac catatcctgg
     5521 aaacaggtgt gagccacgtg atcctgaccc tcgacgaagt ggtcagtgat tataccatca
     5581 tcctcaacac cctccagaag gatgtgtcca actctgagct cgaggccgtg cagcagatat
     5641 accgtcagct gcgtggtgct gaaccacctg atgatgaaac cgcccgcggt atcatcgaca
     5701 aactgttctt ctcagacaag cgctacgacc ttggggaagt aggccgctat aagatcaacg
     5761 agaaactggg gatggaaacc tccctggata ccaaggtcct gaccaaggcc gacatcattg
     5821 ctattgtcaa atatctcgtt gagctttcta acaacaaagc agagatcgac gacattgacc
     5881 acctgagcaa ccgccgggtg cgtactgttg gcgaacagct ctatgcacag ttcggtgtgg
     5941 gtctggcacg tatggctcgt accatccgcg aacggatgaa cgtacgtgac aatgaagtgt
     6001 tcaccccgat cgaactgatc aatgccagaa cgctgtcttc tgttatcaat tccttcttcg
     6061 gtaccagcca gctgagccag tttctggatc aggtcaaccc gctggcagag atcacccata
     6121 aacgccgtat ctcagctctc ggacctggtg gtctgagccg tgaacgtgcc ggtttcgagg
     6181 tgcgtgacgt acactacagc cactatggcc gcctgtgtac catcgaaacc ccggaaggtc
     6241 cgaacatcgg tctgatctct accctgtgcg tccatgccaa gatcaacaag atgggcttca
     6301 tcgaaacgcc ttatcgcaaa gtggtcaaag gcaaagtgga agccaagggt gaagtggtgt
     6361 acctgaatgc agaagaagaa gaccatatgg tcattgccca ggccaatgcc aaggtagatg
     6421 acaaaggtaa tttcatcaac gaccgcgtga agtgccgtga acagggtgat tttcctgaat
     6481 acgaacccga tcgcatccaa tatatggacg tagcccccaa ccagatcgta ggggtttctg
     6541 cttccatgat cccgttcctc gaacacg
//
LOCUS       DAOMAX010000144         6510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1879_length_6510_cov_3.534469, whole genome shotgun sequence.
ACCESSION   DAOMAX010000144 DAOMAX010000000
VERSION     DAOMAX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_6510_cov_3.534469"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..2015
                     /locus_tag="PK742_08295"
     CDS             135..2015
                     /locus_tag="PK742_08295"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76631.1"
                     /translation="MIKWTLALLLIACQQLVFSQTQEIDSLLQKLSAARPDTNKVIVL
                     RRLAATVDEYDPAKAIAYGREGIALGRELGYLKGVAGCYLNIGMIYVQLSKFDSAIVY
                     CDSAILVSEEAGDPKRIALAYINRADLYTQIRQFEKAHLDCRNALEYARASDDPDRIG
                     RVYMQSGVIYFYQEQYEKALEYYNTSLEYFGDVGYSGVAAAVMANIAEVFVSQGKYQE
                     ALKESERAIVLADSLQLLYYLTSFYGNHGEIYMHLKDHANAEKYYKLALELAVSTENI
                     SDIALNRNRLGLLYMDMRDPERAIPVLQSGFELTNRSHFALERQQLAQSLSEAYAATG
                     KYDLAHKYLRISSDLRDSLHTSETNRDILLLQTEFDLADKERRIDLLNANDEIQRNRI
                     RQQQLILYFASGLIILVIIVLLLTINRRRIKQELHELSLRNSIALDLHDDVGGSLSSI
                     RMLADIAAAHQTPADLLQRISVNAAETIERMSDIIWMINPRYDDLESLVHRLERFMTD
                     LKGADMRTSIEVAKGLSANMRMQERKELLLIVKEAIHNAIKYAGGGSLHVVFSARGKD
                     LVVEVTDTGIGFDLQQTERGHGLDSMQARAKGIGAGLQVISSPGAGTKIILRIPDVAE
                     IS"
     gene            2068..2706
                     /locus_tag="PK742_08300"
     CDS             2068..2706
                     /locus_tag="PK742_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU76632.1"
                     /translation="MNTISVAIFEDNKHLRESLSILINGTPGYRCTGAYPDCQHLIPR
                     LRSATPDVILLDIEMPGMNGLEALKVIREEFPSVQVLMQTMFDDDDYIFRAICSGAHG
                     YILKSTSPAGYLESVADVHQGGAPMSSTVARKVLTLFRNHQPAESADYQLTAREREIL
                     SHMVEGKSFKMIAGDMDIAYETVRTHMKNIYSKLHVNSNTEAVSKAIRERLI"
     gene            2790..4748
                     /locus_tag="PK742_08305"
     CDS             2790..4748
                     /locus_tag="PK742_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76633.1"
                     /translation="MRKVIITWLLLTGVILEMSGQTNTFPGSGSVGIGTLSPSGSSAL
                     EIQSTDQGILIPRMTKAQRDAIASPATGLLIYQSNSQPGFYYFNGSMWVSLLAKGANL
                     SLSNLAATTAVNSSLLPFADQVIDLGSSSRHWQKAFVETIQFADGSTMTTAPDDSLIF
                     ITGTGLQMTGTTLELANTSVVPGVYGSTTFIPQITVDAQGRITSVTDVAAAGGGSESD
                     PQVGVISTDYTPRWNGTELVTGKIRDNGARLAVGGDFITSSKATFYNRESVFSAEQSA
                     VRAVDRTVSGAGVATDYGTAMLGYAPDIPGLLPVSADHIAVYGSQLSGGGTGAALYGI
                     SNGNGSANYAVVATSTATGSLNFGLKSLVTGSGMTNRAVWAEASGGSFGNYAITVPEN
                     GGRSGFGWTTPNAIVGIRHDGSDDPFRIVNNDGSTNLVVDTEGRVGINMDPDAFSASF
                     NVSGSQYISNKLAIGVSPATVFTPLTVHGDNEVVTVEGNDPYIQLKDAGNKIGYLRAL
                     GSDLQLATNAENDFGKLIFRTNGVNQMYINENGSIVIGSTSVIPATGYKLSVDGKIMC
                     EELRVEMSPWPDYVFHDEYELLPLHELSAFIASNQHLPGIPAASEIESEGLEVGEMQA
                     MLMEKVEELTLYIIELQRQIDELKATDY"
     gene            4768..6474
                     /locus_tag="PK742_08310"
     CDS             4768..6474
                     /locus_tag="PK742_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQU76634.1"
                     /translation="MRYNLKISLLLAAIMAIATGYPQQESRPGCLITPDEMRAYTDEV
                     RMQTDALKQADKLHFYPVERLDADSISIEKFLDLNYLGWPLRNANYSDFPGWAIGNFV
                     DLDYYSDGDEDYFEDSETEDYECGHRTYDGHQGIDIGIHPYHWKAQDDGAVEVVAAAS
                     GVIVDKREGYYDEYCVWGGAANSRGNSIVILLDDTSTATFYMHMKDGSLTSKVEGDYV
                     QKGEYLGTVASSGNSTGPHLHFQINYLYEDDPDGDFTGYNQDAMVGACNDGSYFAAFE
                     EQPPYDDPAVLTLETHSSSPELYEDNCDKDIGLYYDNSFTGFATVYFRSKFRDWVDGS
                     SITHWIYYPSGLVKTFYTRTNPTDNRTFFAPDQAVGFSPFDESGTYRYTVEMDGETYS
                     HYFAYNCIASQTLSGARSGHGGNMVSFSINSTQTISGSSANYIQYMADEYVQLNPGFE
                     AAAGCRFVANLKGCNNSTAFAETADATRQVAPESIFSELQLYPNPSMGMVNLYWPDAG
                     MEQAALRVLNQRGEIVYQSNISSSDGLMATVLDLQDLAKGVYMIQLTGDQQTIVRQLV
                     LQ"
BASE COUNT         1759 a         1446 c         1633 g         1672 t
ORIGIN      
        1 ggttgcaggc atttttcgaa ggcgaatacc tatttgcagg aacatggtat gcaaaagata
       61 gactggaagt tggctaactg aggccacttt taatgttaca taggtaaaag tatatttacc
      121 tcctgcattt caccatgatc aagtggaccc ttgccctcct gctgatagcc tgtcagcaac
      181 tggtcttttc tcagacacag gagatcgatt cattattgca aaagctttca gctgcaagac
      241 ctgacaccaa taaggtgatc gtgctcagaa gactggcagc aacggtagat gaatatgacc
      301 cggcaaaagc gatagcctac ggaagagagg gaattgcact tggaagagag ttgggttatt
      361 tgaaaggagt tgccggttgt tacctcaaca tcgggatgat ctatgtacag ctgtcgaagt
      421 tcgattctgc catagtttat tgcgattctg ccatcctagt atctgaagaa gccggagatc
      481 caaagaggat agctttggcc tatatcaacc gggcagacct gtacacccag atcagacagt
      541 ttgaaaaggc tcaccttgat tgccgaaatg ccctggagta tgccagagcc tctgatgatc
      601 cggatcgcat tggccgtgtc tatatgcaaa gcggtgtcat atatttttat caggaacaat
      661 atgaaaaggc attagaatac tacaacactt cactggagta ttttggagat gtaggatatt
      721 caggagttgc agcagcggtc atggccaata tagcagaggt gtttgtatcg caaggtaaat
      781 atcaggaggc actgaaagaa agtgaaagag cgattgtcct tgcagatagc ttgcagctgt
      841 tatattatct gaccagtttc tacggaaatc acggtgagat ctacatgcat ctgaaagatc
      901 atgccaatgc agagaaatat tataagcttg cacttgaact ggctgtttct actgagaata
      961 tcagtgatat agccctgaat cgcaacaggc tgggtctgct ttatatggac atgcgggatc
     1021 ccgagcgtgc cattccagta ctgcagtccg gttttgaatt gacaaacaga tctcactttg
     1081 cattggagcg gcaacaactt gcacaatcct tgtctgaagc atatgcagct acaggcaaat
     1141 atgatctggc gcacaaatat ctgcgcatca gcagtgacct acgcgacagt ctccatacct
     1201 ccgaaacgaa cagagatatc ttattgctgc aaaccgagtt cgatctggct gataaggaac
     1261 gcaggatcga ccttttgaat gccaatgatg aaattcagcg gaacaggatc cggcagcagc
     1321 aactcattct gtattttgct tccggtctta tcatcctggt cataattgtg ctgctgctta
     1381 ccattaacag acgaaggatc aagcaggagc tgcatgaatt atcattgcgc aacagtattg
     1441 cactggatct gcatgacgat gttggtggct ctctgagcag tatcagaatg ctcgcagata
     1501 tagcagccgc ccaccagacc ccggctgatc tgttgcagcg tatttctgta aatgccgctg
     1561 agaccattga gcgaatgagc gatatcatct ggatgatcaa tccgcgatat gatgatctgg
     1621 aaagtctcgt acacaggctt gagagattca tgactgacct gaaaggtgca gacatgagaa
     1681 ccagtatcga agtggccaaa gggctatctg ccaatatgcg catgcaggag cgaaaggaac
     1741 tgttgctcat tgtcaaggag gccatacaca acgccatcaa gtatgcaggc ggcggctctt
     1801 tgcatgtggt attttcagca agggggaaag atcttgttgt ggaggtaacg gataccggaa
     1861 ttggttttga cctgcagcag actgaacggg gccacggtct ggacagcatg caggcaaggg
     1921 ccaaagggat tggcgctggg ttgcaggtga tcagttctcc tggagccggc acgaagatca
     1981 tacttcgaat accggacgtg gcagagatca gttaacccta ttgcagtata tataatcttc
     2041 tgtcgatcat ccaaatttga accaccattg aataccatat ctgtagccat atttgaagac
     2101 aacaagcacc tgcgggaaag tctgtccatt cttatcaatg gtactccggg ctatcgctgc
     2161 acaggtgcat accccgattg ccagcatctc attcctcgac tcagatcagc aactcctgat
     2221 gtcatcctgc tggacattga aatgcccgga atgaatggcc tggaagcgct gaaggtcatt
     2281 cgtgaagaat ttccctcagt tcaggtgttg atgcagacca tgtttgacga tgatgattat
     2341 attttccgtg caatttgttc cggtgcgcat ggctatattc ttaaatccac ttcacctgcc
     2401 ggctatctgg aatctgttgc tgatgtccat cagggaggag ctcccatgag cagcacggtg
     2461 gcaagaaaag tgctgacctt attccggaat caccagccgg cagagtctgc agattatcag
     2521 cttacagcca gagaacggga gatcctgagc catatggtgg aaggtaagag tttcaagatg
     2581 atagccggcg atatggatat agcttatgag acagtccgca cacatatgaa gaacatctac
     2641 tctaaactgc acgtgaattc caatacagaa gcagtttcca aagcaatcag agaacgactc
     2701 atctgatctg cactaacccg attgtggtat ttcagccgtg aggtgacctg gatagttttg
     2761 cttctatcta atcaccttca atttctacta tgagaaaagt aatcattact tggttattgc
     2821 tgacaggcgt gatcctggaa atgtccggtc agaccaacac gtttccaggt tctggaagtg
     2881 tgggtattgg taccctcagc ccctctggtt catctgcact ggagatacaa tcaactgatc
     2941 agggaattct tattccaagg atgaccaaag cccagcgtga tgccattgct tcacctgcca
     3001 ccgggctact tatctatcaa agtaattcac aacccgggtt ttattatttc aatggatcca
     3061 tgtgggtctc tttgctggct aaaggtgcca atttatctct ttccaatctg gcagcaacta
     3121 cggcggtaaa cagttcactg cttccttttg ccgatcaggt cattgacctg ggttcttcct
     3181 cccggcattg gcaaaaagcc tttgtggaaa ccatccagtt tgctgatggc agcacaatga
     3241 ccactgcacc tgatgatagt ctgatattta ttaccgggac aggtctccag atgacgggta
     3301 ccaccctgga gttggccaac acatcagttg ttccaggtgt gtatggcagt acaactttca
     3361 tacctcagat tactgtagat gctcaaggga gaattacatc cgtaactgat gttgccgcag
     3421 cagggggtgg ttcagagtca gacccgcagg tgggtgtcat cagtacagat tacacaccca
     3481 ggtggaacgg aaccgagctg gttaccggca aaataagaga caatggtgcc cggcttgcag
     3541 tgggtggcga ctttattact tcctccaagg caacctttta taacagggaa tctgtttttt
     3601 cagctgaaca atcagcagta agggcagttg acagaacggt cagcggagct ggggttgcta
     3661 cagattacgg aacagcaatg cttggctatg cgccggatat tcccggattg ttaccggtat
     3721 cagcagatca tattgcggta tatggcagcc agttgagtgg tggaggaacc ggggcagcgt
     3781 tatacggtat aagcaacggc aatggttctg ccaattatgc agtggtggca acttctactg
     3841 cgacaggtag tctcaatttt ggtttgaaat cattggtcac tggtagtgga atgaccaacc
     3901 gtgctgtctg ggcagaagca tccggcggat cttttggcaa ctatgccatt accgttccgg
     3961 agaatggtgg ccgttccggt ttcggctgga ccacacccaa tgccatagtg ggcatcaggc
     4021 acgatggctc tgatgatccg ttccgtattg tcaacaacga tggcagcacc aacctggtag
     4081 tagatactga aggcagggta ggtatcaata tggatcctga tgcattcagt gccagtttca
     4141 acgtaagcgg aagtcagtat atatcgaaca aacttgccat aggtgtcagc cctgccacag
     4201 ttttcactcc attgacggtt catggcgata atgaagtggt cacagtagaa ggaaatgatc
     4261 cttatatcca gctgaaagat gccggaaaca aaattggtta tctccgggcg cttggatccg
     4321 atctgcaact tgccaccaac gcagaaaatg atttcggcaa gctcattttc cgtaccaatg
     4381 gcgtaaacca gatgtatatc aacgagaatg gaagtattgt cattggatcg acctctgtta
     4441 tccccgcaac cggttataaa ctcagtgtgg acggcaagat catgtgcgaa gaattgcgtg
     4501 tggaaatgtc accctggccc gattatgtgt ttcatgatga atatgaactg cttccattac
     4561 atgaactgag tgcattcata gcatcgaacc aacacctgcc tggaattcct gccgcaagcg
     4621 agatcgagtc agaaggactg gaggtcggtg agatgcaggc catgctgatg gaaaaagtgg
     4681 aagagctgac cctctatatc atagagctgc agcgccagat agatgagctg aaagcaacag
     4741 attactgatc cccaaaagga actgatcatg agatataatt tgaagatctc tctgttgctt
     4801 gctgccataa tggcaatagc aacaggatac ccacagcagg aaagcagacc aggctgcctg
     4861 atcactccgg atgagatgcg ggcctataca gatgaggtca ggatgcagac agatgccctg
     4921 aaacaggcag acaaacttca tttctatccc gttgaacgtt tggatgcgga ttctatatca
     4981 attgaaaaat tcttggattt gaattatctg ggatggccac tacggaatgc caattacagc
     5041 gactttccgg gctgggccat tggcaacttt gtagatctgg actattattc tgacggagac
     5101 gaagactatt ttgaggactc tgaaacagaa gattatgaat gcggacaccg tacctatgat
     5161 ggtcatcagg gtattgacat agggatacat ccctatcatt ggaaggccca ggatgatggt
     5221 gcggtggaag tggttgcagc agcctccggt gtcatagttg acaaacgtga agggtactat
     5281 gatgagtact gtgtttgggg cggagccgcc aacagcaggg gtaacagtat tgtgatcctg
     5341 ctggatgata ccagtacagc tactttttat atgcatatga aagacggttc gctcacatca
     5401 aaagtggaag gtgattatgt gcaaaaagga gagtatctgg gtacagttgc aagctctggg
     5461 aactccaccg gtcctcacct gcatttccag atcaactact tatatgaaga tgatccggac
     5521 ggcgatttta ccggatataa tcaggatgcc atggtgggtg cgtgtaatga tggaagttat
     5581 tttgcagctt tcgaagaaca acctccatat gatgacccgg cagtactgac cctggagaca
     5641 cattcctctt cgcccgagct ttatgaagat aattgtgata aggatatcgg gctgtactac
     5701 gacaattcat tcacgggatt tgccactgtg tatttccggt caaaattccg cgactgggtg
     5761 gacggttcat ccatcaccca ctggatctat tatccttccg gtctggtaaa gaccttttat
     5821 acaagaacca atcctacaga caacagaacc ttttttgcac cggatcaggc tgttgggttt
     5881 tcacctttcg atgaatcagg tacatacagg tacactgtag aaatggacgg cgagacctat
     5941 tcccattatt ttgcctacaa ctgcattgcc agtcagacac tcagcggtgc caggtccggg
     6001 cacgggggga atatggtgag cttttctata aattctaccc agaccatatc cggttcttct
     6061 gctaattaca ttcagtatat ggccgatgaa tatgtgcagc tgaatccggg ttttgaagcg
     6121 gccgcaggtt gtcggtttgt tgcgaatcta aaaggatgta ataacagtac tgcatttgca
     6181 gagacagcag atgctaccag gcaagtggca ccagagtcta tattttcaga acttcagctc
     6241 tatccgaatc cttctatggg aatggtcaac ctgtactggc ctgatgcagg catggagcag
     6301 gcagctctgc gggttctgaa ccagcgggga gagatcgtgt accagagcaa tatttcttct
     6361 tccgatggac ttatggccac agtgcttgat ctgcaagacc ttgcaaaagg agtgtatatg
     6421 atccagttga ccggagatca gcaaacgatc gtcaggcagc tggttctgca ataaaagaaa
     6481 aagcgggatg aatttcatcc cgcttttttt
//
LOCUS       DAOMAX010000145         6496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1884_length_6496_cov_4.106039, whole genome shotgun sequence.
ACCESSION   DAOMAX010000145 DAOMAX010000000
VERSION     DAOMAX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_6496_cov_4.106039"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PK742_08315"
     CDS             <1..801
                     /locus_tag="PK742_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQU76635.1"
                     /translation="NHYLVLPVLAYLCCLKEDSNVSNFNEEKHSWESPVLGLKMEMLT
                     FGTEGFPIVLFPTSMGMHNENRDFKLIEAIGWFIDQGMIKVYCPDGIDKHSWYNTDAA
                     PEYRVKNHILYDQMLTEELYPRMKEETGFSRIGTAGCSFGGYHAMNFGLRHPDLVAAI
                     FSLAAKFDIRDQMDGVYNDDVYFNNPVDYLPDLEDPWLWKSQIFLGSAEYDMCLDANY
                     NLAHVLGTKQVPHMLQVKPNDKHDWPCWREQLPYFLSLLDIEKTKELL"
     gene            832..1833
                     /locus_tag="PK742_08320"
     CDS             832..1833
                     /locus_tag="PK742_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76636.1"
                     /translation="MEIKKIGILFGQEDSFPNAFVDTINSRNVPGIVAEKVSIDKVMQ
                     GEPTEYTVILDRISHDAPFYRAYLKNAAMSGTYVMNNPFWWSADEKFFNNALAVKLGI
                     PVPKSVLLPSHDMPPNTKEGSFRNLKYPMDWQSIFNYTGLPAYMKPHDGGGWRGVTKI
                     ESIEQFFEVYKDSGTDVMMLQEEITFDYYFRCYYLAGDRVHIMYYEPRNPHHLRYTWN
                     GPQASAELMETVRKYTIALNQGLGYDFNTVEWAVRDGIPYAIDFCNPAPDAERLSVGE
                     ENFAWIIQNAADMAIEKALNHKPGVVNLSFGTFIKNSVYGFPHKGETLPIFSAASTN"
     gene            1836..2942
                     /locus_tag="PK742_08325"
     CDS             1836..2942
                     /locus_tag="PK742_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HQU76637.1"
                     /translation="MIDLSPFTLGIEEEFQIIDPVSRDLAHRRHHIIDAGEATGEQIK
                     AEMHQSVVEVATKICADIKEARQEVFKLRRIVADLADKDNLKIAAAGTHPFAQWEEQL
                     ITNHPRYEEIINEMQDAARSNLIFGLHVHVGMPSRDVAVFMMNSLRYFLPHIFSLSTN
                     SPFWEGRNTGFKSFRTKVFDKFPRTGLPDVFNDASHFDNFVALLVKTNCIDNGKKIWW
                     DLRVHPYFNTIEFRICDVTLRADETVALAALMQALTAKLYKLMRSNMGWRNYDKALIN
                     ENKWRAARFGIEGNLIDFGKQKEVNTKDLILEVIELVDDVLDELGSREEMEHIKWILN
                     NGTGADRQLESYNQNGYNFKKLMDFIVEESYVGL"
     gene            complement(3011..3667)
                     /gene="fsa"
                     /locus_tag="PK742_08330"
     CDS             complement(3011..3667)
                     /gene="fsa"
                     /locus_tag="PK742_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQU76638.1"
                     /translation="MKFFIDTANLEEIKEAAALGILDGVTTNPSLMAKVGVSGEENIR
                     NHYKAICEIVDGDISAEVIATDYDGIIREGKELAALHPNIVVKVPMIKDGVKAIRWFT
                     DNGIRTNCTLVFSAGQAILAAKAGATYLSPFIGRIDDINWDGMDLIAQIVHIYNVHGF
                     DTQVLAASIRNPMHIVKCAEAGADVVTCPLSSIMGLFKHPLTDIGLAQFLADHKKANG
                     "
     gene            complement(3737..3835)
                     /gene="ffs"
                     /locus_tag="PK742_08335"
     ncRNA           complement(3737..3835)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK742_08335"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3906..5534
                     /locus_tag="PK742_08340"
     CDS             3906..5534
                     /locus_tag="PK742_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQU76639.1"
                     /translation="MNLEQNRNEDSMRLQISRMESRLGEIMLGGGKKNIEKLHAKGKM
                     TARERIDALLDPGSERLEIGGFAGYDMYQEQGGCPSGGVVVVMGYVSGRQCLVVANDA
                     TVKAGAWFPITGKKNLRAQEIAMENRLPIIYLVDSAGVFLPMQDEIFPDKENFGRIFR
                     NNAIMSSMGITQIAAIMGSCVAGGAYLPIMSDEAIIVKKTGSIFLAGPYLVKAAIGEE
                     IDAETLGGAHTTNAISGICDYEAEDDADCLLKVREIMGKLGAPASAGFDRIKPAAPAL
                     DELEILGTIPEDPSKPYDMEIIIRRLVDDSQFEEYKAEYGKSILCGYARIDGWAVGIV
                     ANQRKVVKNAKGELQFGGVIYSDSADKAARFIMNCNQKKIPLVFIQDVTGFMVGSKSE
                     HGGIIKDGAKLVNAVANSVVPKFTIIAGNSFGAGNYAMCGKAYDPRLIVAWPNARIAV
                     MGGAQAAKTLVQIEVASLKAKGKEISPEDEQQMLADITAKYDEQMSPYYAAARLWVDG
                     IIHPLDTRKWVSMGIEAANQSPIEKAFSTGVIQV"
     gene            complement(5699..6130)
                     /locus_tag="PK742_08345"
     CDS             complement(5699..6130)
                     /locus_tag="PK742_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76640.1"
                     /translation="MYKPVFLLLVALPGWLSCSPRFAKTFEEQWVRNGMYTEVDFERI
                     RLVCTFPGDVEISTSKQEIRQAIDGRGYRNILLYGKTSIEPYYEIFVLQDPADVPSFD
                     LVCDTLFYGKHLVIGGNGLADPIPVSDFRFILDNLKVFYPK"
     gene            6170..>6496
                     /locus_tag="PK742_08350"
     CDS             6170..>6496
                     /locus_tag="PK742_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76641.1"
                     /translation="MRHLIFSLLVLLTLTMTAQENRLIPAPAEWSPETGSFNLTPTTF
                     LLARDMQAFQDAVTFRDICIDWFDFPLKTAIKEIGSGPYIEVAYDSTLDLPDGGYILD
                     IAPEGIK"
BASE COUNT         1750 a         1565 c         1633 g         1548 t
ORIGIN      
        1 aatcactacc ttgttctgcc tgtcttggcc tacctttgct gcctcaaaga agatagcaac
       61 gtgagcaatt tcaacgaaga aaaacacagc tgggaaagcc ctgtactcgg gctgaaaatg
      121 gaaatgttaa cattcggcac agaaggtttc cctatcgtgc tgtttcccac ctctatgggc
      181 atgcacaatg agaaccgcga ttttaagctc atcgaggcca tcggctggtt catcgaccag
      241 gggatgatca aggtgtattg cccggacgga attgataaac acagctggta caatactgat
      301 gcagcccccg aatatcgtgt caaaaatcat atactctatg atcagatgct gacagaagaa
      361 ttgtatcccc ggatgaagga agagaccggt ttttcgcgga taggaacagc aggatgcagt
      421 tttggcgggt atcatgctat gaatttcggc ctccggcacc ctgatctggt tgctgccata
      481 ttttcccttg cggcaaagtt tgacattcgc gatcagatgg atggggtgta taacgatgat
      541 gtttacttca acaatccggt agattatctg ccggacctgg aggatccctg gctctggaaa
      601 tcgcagatat tcctgggttc tgctgagtat gacatgtgcc tcgatgccaa ctataatctg
      661 gctcatgtgt tgggtaccaa acaagtgcct cacatgctgc aggtgaagcc gaatgacaag
      721 catgactggc cttgctggag agagcagctc ccctactttt taagtctgct ggacattgaa
      781 aaaaccaaag aacttcttta aagcaactaa taaataaaat cccataataa aatggaaatc
      841 aagaaaatag gaatattgtt tggtcaggaa gacagcttcc ccaatgcctt cgtagatacc
      901 atcaacagca ggaatgtacc cggcatcgtt gcagaaaagg tcagcataga taaggtcatg
      961 cagggtgagc ctacagaata taccgtcatt ctggatcgca tcagccacga tgctccattc
     1021 tatcgtgcct atctgaagaa tgccgccatg agtggcacct atgtgatgaa caatccgttc
     1081 tggtggagtg ctgatgaaaa attcttcaac aacgccctgg cagttaaact gggaattcct
     1141 gtaccgaagt cagtgctgct tccctcccat gatatgccac ccaataccaa ggaaggatca
     1201 ttccgcaacc tgaagtaccc gatggactgg caatcgatct ttaattatac cggacttcct
     1261 gcctacatga agccgcatga tggtggtgga tggagaggtg tgaccaagat cgaatccatt
     1321 gaacagttct ttgaggtcta taaagacagc ggtaccgacg tcatgatgct gcaggaagag
     1381 atcacttttg attactattt ccgatgctat tacctggctg gcgaccgtgt gcatatcatg
     1441 tattacgaac cacgcaaccc gcatcacctg cggtacacct ggaacgggcc gcaggccagt
     1501 gcagagctga tggaaactgt tcgcaagtac accattgccc tgaaccaggg actcggctat
     1561 gacttcaata cagttgagtg ggcggtcaga gacggcattc cttatgccat tgacttctgc
     1621 aatccggctc ctgatgctga gcgcttaagt gtgggtgaag agaactttgc ctggatcata
     1681 cagaacgcag cagacatggc cattgagaaa gccctcaacc ataaaccggg cgtggtcaac
     1741 ctgagctttg gcaccttcat aaaaaattct gtgtatggtt ttccgcataa aggagaaacc
     1801 ctgcctatat ttagcgcagc aagcacgaat tgactatgat cgacctgtcg ccatttacct
     1861 taggaataga agaagagttt cagatcattg atcctgtttc ccgcgatctc gcccatcgca
     1921 ggcatcatat cattgatgcc ggtgaggcta ccggtgagca gatcaaggct gagatgcacc
     1981 agagtgtggt agaagtggcc accaagatct gtgcagacat caaggaagca cgccaggagg
     2041 ttttcaagtt acgcaggatc gtagctgacc tggcagataa agacaacctg aagatcgcag
     2101 cggcaggaac ccaccccttc gcccagtggg aagagcagct cattaccaat catcccaggt
     2161 atgaggagat catcaatgag atgcaggatg ctgcacgaag caatctcatc ttcggcttgc
     2221 atgtgcatgt aggaatgcct tcaagagatg tggcagtatt catgatgaac tccctgcgtt
     2281 acttcctccc ccacatcttt tccctcagca ccaacagccc tttctgggaa ggccggaata
     2341 cagggttcaa gagcttccgc accaaagtgt ttgacaagtt tccccgaaca ggattacccg
     2401 acgtgttcaa cgacgcaagt cactttgaca atttcgtggc gttgctggtc aagaccaact
     2461 gcatagacaa tggtaaaaag atctggtggg atctgcgggt gcatccttat ttcaatacga
     2521 tcgaattccg catctgcgat gtaacactgc gtgccgatga gaccgttgct ctggcagcac
     2581 tcatgcaggc acttactgcc aagctgtaca aacttatgcg cagcaatatg ggatggcgca
     2641 actatgacaa ggcgctcatc aatgaaaaca aatggcgtgc agcccgattt ggtattgagg
     2701 gcaacctgat cgattttggc aagcaaaaag aagtcaacac caaagacctc attctggagg
     2761 tgatagaact ggtggatgat gtgctggatg aactaggcag ccgggaagag atggaacaca
     2821 tcaaatggat cctgaacaac ggcaccggtg cagacagaca gctggaaagc tataaccaga
     2881 atggctataa ctttaagaag ctgatggatt tcattgtgga ggaaagctac gttggcctct
     2941 gacgaagtcc tgaacagaaa caaccaattc cttatttcat tctgcagaca acccttcggt
     3001 aaatctgtaa ttagccgttt gctttcttgt gatctgcaag gaactgtgcc agtccgatat
     3061 cagtgagcgg atgtttgaac aatcccatga tggaactgag cggacaggtc accacatcgg
     3121 caccagcctc tgcacatttg acaatatgca tggggttgcg gatagatgcc gccagtacct
     3181 gtgtgtcaaa tccatgaaca ttgtatatgt gcacgatctg tgcgataagg tccatcccat
     3241 cccagttgat atcgtcgata cggccaatga acggcgacag ataagtggcc cctgccttgg
     3301 cagccaggat tgcctgacct gcactgaata cgagtgtgca attggtgcgg ataccgttat
     3361 cagtaaacca tctgatagct ttcacgccat ctttgatcat gggtactttc accacgatgt
     3421 tcggatgtaa ggctgccagt tctttcccct ctctgatgat gccatcatag tcagtagcga
     3481 tcacctcagc actgatatct ccatccacga tttcgcaaat ggctttgtaa tggttacgaa
     3541 tgttctcttc gccggatacc cccaccttcg ccatcagcga tggattggtg gtcaccccat
     3601 ccagaatgcc cagcgcagcg gcttctttga tctcttccag attggctgta tcaataaaga
     3661 atttcatagt atttttttac agggataaaa gtaatgtatg aaagatatga tcaaaaatga
     3721 caggttgtgc aaaaaaaatg ggcaagttac ttacatcgca ccgctacgac cacctaccct
     3781 tgcttccgtc aggacctggg ggattcagca ggagctggtc gtataagact tgcccggtgc
     3841 aaagatagca aaaccactgt gcataccgct gccttttgtc ctatttttgc tgttcaggta
     3901 cacgtatgaa tttagaacag aacaggaacg aagacagcat gcgcctgcag atctcccgga
     3961 tggaatccag gcttggagag atcatgttgg gcggtggtaa aaagaacata gagaaactgc
     4021 atgctaaagg caagatgacc gcccgtgagc gcattgacgc cttgctggat cccggaagcg
     4081 agcgtctgga gattggcggc tttgcagggt acgatatgta tcaggaacag ggtggatgtc
     4141 cttccggcgg ggtggtcgtg gtcatgggct atgtatcagg caggcaatgc ctggtggtgg
     4201 ctaatgatgc cactgtaaaa gccggtgcct ggtttcccat cacaggcaag aagaacctga
     4261 gggcacagga aattgccatg gagaacaggc tacccatcat ctatctggtg gacagtgccg
     4321 gagtgtttct tcctatgcag gatgagatat tccccgacaa agagaatttc ggtcgcatct
     4381 ttcgcaacaa cgccatcatg agcagcatgg gcatcaccca gatcgctgcc atcatgggtt
     4441 cctgtgtagc gggcggtgct tatttaccca tcatgagcga cgaggccatc attgtgaaaa
     4501 agaccggcag cattttcctg gccgggccct atctggtcaa agctgccatt ggggaagaga
     4561 tcgatgccga aacactggga ggtgcacata ccaccaatgc catttccggt atctgcgatt
     4621 atgaggcaga agatgatgcc gattgcctgc tgaaggtccg ggagatcatg ggtaagctgg
     4681 gcgcacctgc atctgccggt tttgaccgga tcaaacctgc ggctcctgca ttagacgaac
     4741 tggagatact gggcacgatt cccgaagacc cttccaaacc ttatgatatg gaaatcatca
     4801 ttcgccggct ggtggatgat tcgcagttcg aagaatacaa agctgaatac ggcaagagca
     4861 ttctgtgtgg ctatgcacgg atagacggct gggctgtggg cattgtggcc aaccaacgca
     4921 aggtggtcaa gaatgccaaa ggcgaattgc agtttggcgg tgtgatctac agtgacagtg
     4981 ccgacaaggc tgcacgtttc atcatgaact gcaaccagaa gaagatacca ctggtattca
     5041 tccaggacgt gaccggattc atggtgggca gcaagagtga acatggaggt atcatcaaag
     5101 acggagccaa gttggtgaat gccgtagcca attctgtggt accaaaattt accatcattg
     5161 ccggcaacag tttcggcgct ggtaattatg ccatgtgcgg gaaggcttat gacccaagac
     5221 tgatcgtagc ctggcccaac gcccggatcg cagtgatggg tggagctcag gcagccaaga
     5281 cccttgtgca gatcgaagtg gcaagtctaa aggcaaaggg caaagagatc agcccggaag
     5341 acgagcaaca gatgctggcc gatatcacgg caaaatatga cgagcagatg agtccctact
     5401 atgcagccgc caggttatgg gtggatggta tcatccatcc gctggatacc agaaaatggg
     5461 tgagcatggg gatagaagcc gcgaatcagt cgccgataga aaaggcattc agtacgggtg
     5521 tgatacaggt ataaaaaagc cggcacgcct cctctgcgta ccggctgatt cacaaaacaa
     5581 caaatgtcga tctcacccac agaaatgcgt gatgagccca tttgctactt gcaattgtac
     5641 agatattttc agaattggtt gtcatcagat gttcacaatt aatacatata agtgtcacct
     5701 atttcggata gaagaccttc aggttatcca ggatgaaccg gaaatcagaa acgggtatcg
     5761 ggtcagctaa tccattcccg cctatgacca ggtgtttccc atagaacaaa gtgtcgcaga
     5821 ccagatcaaa tgacggaaca tctgcaggat cctgcaaaac gaatatttca tagtaaggtt
     5881 ctatactggt ctttccataa aggagaatat tgcggtatcc ccggccatct attgcctgtc
     5941 tgatctcctg cttgctggtg ctgatctcca catcacccgg aaaggtgcag accagtctga
     6001 tcctttcaaa atcaacttct gtgtacatgc cgtttcttac ccattgctct tcaaaggttt
     6061 tggcaaaacg gggggaacag gacaaccacc cgggtagtgc taccagcaac aggaatacag
     6121 gtttgtacat gctgcaaatg tataggcaat ctgctacctt cggcgaatga tgcggcattt
     6181 gattttttcc ctgcttgtac tattgaccct gaccatgacc gctcaggaaa accggctcat
     6241 ccctgcccct gctgaatggt cgcccgaaac aggttccttc aacctcaccc ctactacttt
     6301 tcttctggca cgtgatatgc aggcttttca ggacgcagtt acgttccgtg atatctgtat
     6361 tgactggttc gattttccac taaagaccgc catcaaggag atcggttcag gcccttacat
     6421 agaagtggcc tacgacagca ccctggacct gccagacggt ggatacatat tagacattgc
     6481 tcctgaaggg atcaaa
//
LOCUS       DAOMAX010000146         6487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1887_length_6487_cov_4.068874, whole genome shotgun sequence.
ACCESSION   DAOMAX010000146 DAOMAX010000000
VERSION     DAOMAX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_6487_cov_4.068874"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="PK742_08355"
     CDS             <1..2181
                     /locus_tag="PK742_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQU76642.1"
                     /translation="SLARFKTTRYSSVRHFPDNYDPDYWKNLPNEEPFRLPHDLKADL
                     ELKMPLDSQFASYKSEFRKDLPAPCPDTDYYSFYYHGDTITDSLQWMALPSFDSRFRN
                     YLEAENRYAKNVISEDKNYQKKLFDKLNSFYKDQPEKVRINKAGTYFLAEDSLSHEIL
                     CYQIDSGTYREVLDLTQLMEKQENNFLKQVLPDPTNNFILALLETHGTPGSLAYAFSS
                     GSSNPSDSISGVYTIQWFTDSTILYTQTNEAGSSRELHFRNIRNKKDVVIYREKDPKF
                     DVEVSRSNKELFLTVQSKTENEIYLIKPGQEEPVLQLIHGREEGILAKAKDQNGIYLL
                     VQDTPNRSSIKFATLQDPSAQFVYGRSQKGEFIYDFLPVSHGIVALVYDHSIPMLKYY
                     EPENEKWNTIDLNSAIGYYQLLSYDSTANSFRFYFSSPSHPPAAYEYHFETDELKIDS
                     EPTNIDPIYYSYTSTKRIWVKSFDNTMIPVTIVQNRLHGKSHAGLILKAYGAYGALTI
                     PDFSAADAILLEQGYTIAYAHVRGESVLGPSWYQAGRGMQKKNSVEDYIACAEYLIDK
                     EYTTPQKLIGYGNSAGGIVVGQAMNLKPYLFNSVILDHPYLDVINTMMNDSLPLTLDE
                     YKEWGDPNSKEVYDYILSYSPYQNITKQYYPNVLLIASYLDYQTPAWQIAKFAARLRQ
                     QNLSDSKILLLTDMTSGHKGSTTGKEWVKLYAETYTFIKLMCRQ"
     gene            2590..3114
                     /locus_tag="PK742_08360"
     CDS             2590..3114
                     /locus_tag="PK742_08360"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU76643.1"
                     /translation="MHLTYRTGNLQDKDDLQALGILSYGQHQHAMTPENWNKYNSFMS
                     APDTFTYLMGSSTCFLCETDQKIIGMAFLVPHGHPTDMFEEHWSYIRMVGVHPDFGGH
                     GIARKLTQMCISYAIETNEKFVALHTSEFMHAARHIYESIGFRVVRELKRYDKQYFIY
                     LLDLHSYDHDHLPD"
     gene            3095..3502
                     /locus_tag="PK742_08365"
     CDS             3095..3502
                     /locus_tag="PK742_08365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU76644.1"
                     /translation="MITYQIETTLSTDEFRSVLLRSTLSERRPVDDEETLSKMVEHGN
                     LIVTARENGVLIGIARSLTDFNYCTYLSDLAVDESYQKMGIGKELIRQTKLASPKAKL
                     ILLSAPKAVLYYPKIGMTQWEQCYVLDNLEDLK"
     gene            3509..3847
                     /locus_tag="PK742_08370"
     CDS             3509..3847
                     /locus_tag="PK742_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76645.1"
                     /translation="MIDYNGKKFRPVSNTENGETSSETIFLYKQSGNILTSEYSGGKI
                     ITGHLIGKVDDNGCIDMRYHQVNDAGEIMTGTCQSSPEILNNGKIRLHETWQWTSGDR
                     SAGRSILEEI"
     gene            3939..4514
                     /locus_tag="PK742_08375"
     CDS             3939..4514
                     /locus_tag="PK742_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQU76646.1"
                     /translation="MSDAYDKYYQTENLFGEPYPELIRFFTDLTERGSVLDLGCGQGR
                     NALALARLGYTVTGIDISRTGIEQMNKIARSENLALNGTVDNIYTFNRIDAFDIVLLD
                     SIFHFAKKDREKESLLLQKIQSELQPGGLLVICLQDTGNKVKILNQILDSAKKLNKTA
                     DKKFSYLFEDKSNDHSSLTKYRMIVAENKLN"
     gene            4534..5655
                     /locus_tag="PK742_08380"
     CDS             4534..5655
                     /locus_tag="PK742_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76647.1"
                     /translation="MRALTAILILQAASVYAQTDPDELIHYRILPHDTDPLISAFASD
                     VHQAFYNPTSQPQHTLLVHLVGSGDTTGSTTFFPVLAANNGFHCINLDYRNGVSVQSA
                     CADIPDTTCHLNWRKEVIEGIDYSAEIFVNEANSIYNRLFRLLQYLDIHEPTQNWGQF
                     LSDGSIQWDHIIISGHSQGGGHAAVIGISNPVRRVLMFASPNDHIDTLHINAPWSKLL
                     HLAADSDYYSFNAVHDEIADYWKQYEHSQSLGQGAYGDTVPADDASYPYGHSRQLYTL
                     QVAPSGFPIKLTHDIMIRDFETPVDIYGKPVFACVWLYMLGINCDPLVAMTDIPSVQP
                     ERQLLRILDLLGRETEFQPNEILIYQFSDGSTEKKIIIQ"
     gene            5792..>6487
                     /locus_tag="PK742_08385"
     CDS             5792..>6487
                     /locus_tag="PK742_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76648.1"
                     /translation="MIRFPLILLLMICADAFGQKITYNYLVDSTDNDTREVMTLFENY
                     LHNRPQDQTENHYWNTDDQQKYLQFDFLESEFQPSLYMGFPVHVLSITFRKEICEIKA
                     QFSSCKADGSPYVLAIVNYIAKKEDGSYRLYNALTWNRQDWEQYSVGIVDFYYPDYHK
                     FDHEKAEKLHTFILDLCSHLGVPPKPLEYYLADTYDEIQKLKGLDYYLGMGGQSLPSG
                     RASDNKVYCAGLGE"
BASE COUNT         2047 a         1453 c         1330 g         1657 t
ORIGIN      
        1 agtttagccc ggttcaaaac aacccgatat tcttcagtac gtcattttcc agacaactac
       61 gatcctgact attggaaaaa ccttcccaat gaagaaccct tcagactgcc tcatgatctg
      121 aaagcagatc tggaactgaa aatgcccctt gattcccaat tcgccagcta caaaagcgag
      181 ttcagaaaag atctgccggc accctgtcca gatacagatt actactcatt ttactatcac
      241 ggcgacacta ttacagacag tctgcaatgg atggctctgc catcattcga ctcgaggttc
      301 agaaattatc tggaggcaga gaacaggtat gcaaaaaatg ttatttccga agacaaaaat
      361 tatcagaaga aactatttga taagctaaac tctttttata aggatcaacc ggaaaaagtc
      421 agaataaaca aagccggaac ctacttcctt gccgaagaca gcctgagcca tgagattctc
      481 tgctatcaga tcgactcagg cacatacaga gaagtccttg acctgacaca attaatggaa
      541 aagcaggaga acaactttct taaacaggtc ttaccagatc ccacaaataa tttcatcctg
      601 gcacttctgg aaacccatgg cacccccggc agtctggcat atgcattttc ttctggcagc
      661 agcaatcctt cagattccat ttcaggggtt tacaccatac aatggttcac tgattcaaca
      721 attctttata cccaaacaaa tgaggctgga agttcccgcg aacttcattt tcgcaatatt
      781 cgaaacaaaa aagatgtggt catctacagg gaaaaagacc cgaaatttga tgtggaagtg
      841 tcacgatcca acaaggaatt attccttaca gtacaatcta aaactgaaaa cgaaatttac
      901 ctgataaagc ccggtcagga agagcctgtt ttgcaattga tccacggaag agaagaaggt
      961 atattggcta aagcaaagga tcaaaatggc atttacctgc ttgttcagga tacgccaaac
     1021 cgcagttcaa taaaattcgc aacgctacaa gacccttcag ctcagtttgt ttatggccga
     1081 tctcaaaaag gcgaattcat ttatgacttt ctgccggtta gccatggtat agttgctctg
     1141 gtatatgacc attctatacc gatgctgaag tactatgaac cagaaaatga aaaatggaat
     1201 accattgatc ttaattcagc tatcggatac tatcagctct tgtcctatga tagcactgcc
     1261 aacagtttca ggttctactt tagctctcca tctcatccac ctgctgctta tgaatatcat
     1321 tttgaaaccg atgaactgaa gatcgattca gagccaacga acatagatcc catttactac
     1381 tcctatactt ctacaaagcg aatctgggta aagagctttg ataacactat gataccggta
     1441 accattgtac aaaacaggct ccacggaaag agtcacgccg ggctaattct gaaggcatat
     1501 ggtgcttatg gtgcattgac aatacctgac ttctctgccg ctgacgcaat actgctggag
     1561 cagggatata ccattgcata tgcccatgta aggggcgaat ctgttttagg gccttcctgg
     1621 tatcaggcgg gacgtggtat gcaaaagaaa aattccgtag aagactacat agcatgtgcc
     1681 gaatatctga tcgataaaga atatacaact ccacaaaaac tgattggata cggcaacagt
     1741 gccggcggaa tcgtagtagg acaagcgatg aatcttaagc cgtatctttt taattccgtg
     1801 atcctggatc atccatacct ggatgtaata aataccatga tgaatgacag tttgccactc
     1861 actttagatg aatacaaaga atggggcgac cctaattcaa aagaggtgta tgactatata
     1921 ctttcctatt caccttatca gaatattaca aagcagtatt atcccaatgt cctgcttatt
     1981 gcaagctatc tcgattacca aacacctgca tggcaaatag caaaattcgc tgccagacta
     2041 agacaacaaa atttatccga ttctaagata cttctactga ccgatatgac aagcggccac
     2101 aaaggaagca caaccggcaa agaatgggtc aaattgtatg cagagaccta cacgttcatt
     2161 aaactgatgt gtaggcaata aagatccggt cacgacccgg ttcctctaca aactttcaag
     2221 aacctacagc cagctgaaag tcctgatatg taataacctg accctttctc ccgacaataa
     2281 cgtcttgcct gattcatgct ccttttagaa gactgtgagt gaaatccgga tcctgcagac
     2341 catatgcgat tcatatagtt cagaaacaaa ctgagtcctg cttttccggc atgctttaag
     2401 gtataaggcc tttaaataca ggcatgccag gcctcccccc caccgtctga atcaagcgat
     2461 caaatgatac aattggacaa atcagcggca caagaaataa tgaaacattt caggaaaaac
     2521 tacgttgcac aagatgcccg tatatccaat atctgaaagc cggccataca agttgaaaaa
     2581 caggtaccta tgcatctaac atacagaacg ggcaatctgc aggataagga cgatcttcag
     2641 gccctgggta ttctgtctta tgggcagcac caacacgcta tgacaccaga gaattggaat
     2701 aaatacaatt cctttatgtc agccccggat accttcacat acctgatggg aagttccacc
     2761 tgtttccttt gcgaaacaga ccaaaagatc attggaatgg cattcctggt tccacacgga
     2821 catccaactg acatgttcga agagcactgg agttatatca gaatggttgg tgtacatcct
     2881 gatttcggcg gtcatggtat tgccaggaaa ctgacgcaaa tgtgtatcag ttacgccata
     2941 gaaaccaatg aaaagtttgt agccttgcac acatcagaat tcatgcatgc ggccaggcat
     3001 atttatgaat caattggttt ccgggttgtc agagaactca aaagatatga taaacagtat
     3061 ttcatttatc tattagatct tcattcctat gaccatgatc acctaccaga ttgaaactac
     3121 cctcagcaca gacgaattca gaagcgtcct gttaagatca accttaagtg aaagaagacc
     3181 ggttgacgat gaagagacct tgtctaaaat ggttgaacac ggaaacctga tagtaactgc
     3241 aagagaaaac ggagtactga taggtatagc caggtccctt acggatttta actattgcac
     3301 gtatttgtct gatctggcag ttgatgagag ctaccagaaa atgggaatag gcaaagaact
     3361 gatcagacag acaaaactgg catcaccaaa ggctaaacta attcttcttt cagcacccaa
     3421 agcagtgttg tactatccga aaataggcat gacccaatgg gaacaatgct atgttctgga
     3481 taatctggaa gatctaaaat gacgcaatat gattgattac aacggtaaga aattcaggcc
     3541 ggtctccaat acagaaaacg gagaaacttc ttctgagacc attttcctat ataagcagtc
     3601 aggaaatatt cttacttcag agtactcagg cgggaaaatt attacaggac atctgatcgg
     3661 aaaggtagat gacaacggat gtatagacat gagataccat caggtaaatg atgccggtga
     3721 gatcatgaca ggaacttgtc agtcatcgcc tgaaattctg aataatggca agataaggtt
     3781 gcatgaaacc tggcaatgga cttccggcga cagatcagca ggaagatcga tcctggaaga
     3841 aatataatat ccttactagt cctgttagag ccggcagtgt gtctaacagg aacatccgct
     3901 taagagccaa agccaatagc agaacctgag atacacacat gtctgatgca tacgacaaat
     3961 attaccagac cgaaaacctg ttcggagaac cctatccgga gctgatcaga tttttcactg
     4021 atcttacgga gaggggatca gttcttgatc ttggctgcgg acaaggaaga aatgccctag
     4081 cgctggcaag actgggttat accgttaccg gtattgatat cagtagaacc gggatagaac
     4141 aaatgaacaa aattgcccgt tctgagaacc tggccctgaa cggtacagtt gacaacatct
     4201 acactttcaa ccgaattgac gcttttgata ttgtgcttct ggacagcata tttcattttg
     4261 caaaaaaaga cagggaaaag gaaagcctgt tgctacagaa gatccagtca gaactgcaac
     4321 ccgggggact tttggtgatc tgcctgcaag acaccggcaa taaggtgaag atcctgaacc
     4381 agattctcga ctcagcaaag aagctaaaca agactgcaga caagaaattc tcttacttgt
     4441 ttgaagataa aagcaatgac cattcaagcc tgaccaaata cagaatgatc gttgctgaaa
     4501 ataaactgaa ctgaattcac gaactttatt gcagtgaggg ccttaacagc catactgatc
     4561 ctgcaggcag cttctgtata tgcgcagaca gatcccgatg aactgatcca ttacagaata
     4621 cttccacatg acacagatcc gcttatttca gcgtttgctt ccgatgtaca ccaggccttc
     4681 tacaatccta ccagccaacc acaacatact ttgctggtgc acctggtagg ttcaggcgac
     4741 acaaccggca gtaccacatt ctttcctgtt cttgccgcca ataacggctt tcattgcatt
     4801 aatctggatt atagaaatgg cgtgtctgtg caatcggcct gcgctgatat tcccgataca
     4861 acctgtcacc tgaactggag aaaagaggtc atagaaggaa ttgactacag cgctgagatc
     4921 tttgtgaacg aagccaactc gatctataac agattgtttc gtttactgca gtatctggat
     4981 atacatgaac cgactcagaa ctggggtcag tttctaagcg atggttctat ccaatgggat
     5041 catatcatca tatccgggca ttcgcagggt ggcggccacg cggctgtaat aggtatttca
     5101 aatcctgtca gacgtgttct gatgttcgcc tcacccaatg atcatataga tacgctgcac
     5161 atcaatgcac cctggagcaa gctgctgcat ttggctgcag attcagatta ttacagtttt
     5221 aacgcagtac atgatgaaat tgcagactac tggaagcaat atgaacacag tcagtcactg
     5281 ggacaggggg cctacggcga taccgtaccg gctgatgacg catcttatcc ttacggacat
     5341 tcacggcaac tctatacact gcaggtagcc ccttccggtt ttccgataaa acttacgcat
     5401 gatatcatga tacgtgattt cgaaacaccg gttgatattt acggaaaacc tgtttttgcc
     5461 tgcgtgtggc tatacatgct gggaataaac tgcgatccgc tcgtggcaat gactgacatt
     5521 ccttccgtgc agcctgaaag gcaactactc agaatactgg atctgctggg aagggaaact
     5581 gaattccaac cgaatgaaat tctgatctat cagttcagcg atggaagcac tgaaaagaag
     5641 atcatcattc aataatatat ttccaccttc aaagacctga tgtgtcttga cctccggcac
     5701 accgtagaat tgtctcattt tcatgtcgtg tttgaagata tcagggaaga aaacgggata
     5761 tttgtctctt cttcatatta aattcatacg aatgattcgg tttcctctga tattgttgct
     5821 catgatctgt gcagatgcat ttggacaaaa gatcacctat aactatctgg ttgattcaac
     5881 agataatgat acccgtgagg tcatgacact ttttgaaaac tatctgcata atcgtccgca
     5941 ggatcagact gaaaatcatt actggaacac agatgatcaa cagaaatatc tgcaattcga
     6001 cttcctggaa agtgaattcc agccatcact atacatgggc ttcccggttc atgttctaag
     6061 catcacattc agaaaagaga tctgtgaaat caaagcccag ttctcttctt gcaaagcaga
     6121 tggaagccca tatgtactgg ctattgtaaa ctatatagcc aaaaaagagg atggttctta
     6181 cagactatat aacgcactaa cctggaacag gcaagactgg gaacaatatt ctgtgggaat
     6241 tgtggatttc tattaccctg actaccacaa attcgatcat gaaaaagctg aaaaactaca
     6301 tactttcata cttgacctct gcagccatct gggtgttcct cccaaaccgt tggaatatta
     6361 cctggcagat acttatgatg agatacaaaa attaaaagga ctggattatt accttggcat
     6421 gggcggtcaa tccttaccaa gtggcagagc ttcagacaac aaggtttatt gcgcaggctt
     6481 aggtgaa
//
LOCUS       DAOMAX010000147         6442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1913_length_6442_cov_4.086269, whole genome shotgun sequence.
ACCESSION   DAOMAX010000147 DAOMAX010000000
VERSION     DAOMAX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1913_length_6442_cov_4.086269"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..4180
                     /gene="metH"
                     /locus_tag="PK742_08390"
     CDS             509..4180
                     /gene="metH"
                     /locus_tag="PK742_08390"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQU76649.1"
                     /translation="MEEKWDALKEALSAGILVLDGAMGTMIQRYGLKEEDFRGERFAD
                     WPTDLAGNNDLLSLTRPDIIRQIHTEYLEAGADIIETNTFNANSISQADYGMESLAYE
                     LNAVAARLAREAVEKHRQISPERRVFVAGAIGPTNKTLSLSPDVNNPGFRALDFDTLA
                     VAYMEQINGLLDGGVDLLLIETIFDTLNAKAALSACQQVMDIRGVSIPIMLSGTITDA
                     SGRTLSGQTLEAFLYSVSHAPLLSIGLNCALGARQLLPYVRELADKTGLYVSAYPNAG
                     LPNAFGEYDESAHSHGEDIRHYLEEGLLNIVGGCCGTTPDHIREIARLVTMHSPRKPA
                     TPDKKPQFSGLEALPVFAGSNFINIGERTNVAGSRAFKKLIKENRFEEALQVARQQAE
                     NGAQMLDVNMDDGMINGVEAMTTFLHLLMAEPDIARLPVVVDSSKWEVIEAGLKCLQG
                     RSVVNSISLKEGETEFLRQAKIAKRLGAVVIVMAFDEEGQATTFERRTAICSRAYRLL
                     TEKAGFQPEEIVFDPNILTIATGMEEHNDYGVDFIRTVRWIKENLPGTLVSGGVSNIS
                     FAFQGNDLVREAMHTVFLYHAIAAGMDMGIVNAGMIGIYEDIDLPLKERLEDVLFNRR
                     PDATERLVAFAASMQQGAEIKTAQTDAWRTEDLESRLRFALVHGNTNFIEADTEEALE
                     VLGNPLAVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAWLEPHLLRHKEL
                     SGADQRSRKKILLATVKGDVHDIGKNIVSVVLACNNYDIVDMGVMVPGEKILEKAVEE
                     EVDMIGLSGLITPSLDEMVRVARAMEERGMQLPLLIGGATTSRLHTAVKIAPVYSGVT
                     VHVTDASRCVGVAASLTGEQALAYRKEIDTTYQELREQHARKNNDHNLLPITVARQNK
                     WSFDWNSYTPPLPQRPGTYVFKEEEITVDAIREYIDWTPFFQTWELSGRYPEILTDEI
                     VGEAAGKLWEDAQVMLDTIQQQQWISLQAVIRIERASSTGDDIVVTDEFNQQTMFHTI
                     RQQQVKAGGLPNYALADFIAPKDSGVTDYLGGFAVCAHWKTEQVIRDMEAAHDDYSAI
                     LARALADRLAEALAEYVHQQVRRTYWGYETGDAGTTEDLIREKYQGIRPAPGYPACPD
                     HTEKRTLLDWLNATEHTSIRLTETLAMHPAASVCGWYFSHPESRYFTTGRIGDDQLED
                     LGKRKQMPAASLRNWLGIR"
     gene            complement(4193..5278)
                     /locus_tag="PK742_08395"
     CDS             complement(4193..5278)
                     /locus_tag="PK742_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HQU76650.1"
                     /translation="MSSILTYMEGIQATTLRGRGAQFNPDNRFHAQHRAVLYPEGIDE
                     HEQSSPDTQFFREFPKKILNKVDSPDVGMLWSVNPYQGCEHGCVYCYARNSHQYWGFS
                     AGLDFESRIMVKPEAPRLLEATFMDPKWVPSPVSFSGNTDCYQPAERKWKITRELLKV
                     CLKFRNPVGIVTKNSLILRDLDILQQLAALNLVQVMISMNGIHEKTREKLEPRTATYK
                     KRLEVMETLSSHGIPVQVLVAPVIPGLNDHELPEVIRRAAKHGASDAGYIVLRLNGAV
                     SVIFEDWIRKTFPGMADKVLSQVCDLHGGKLNDSRFGTRMKGEGQWAETIRRMYTITR
                     EKYFGDREKVSLDLSAFRRPGQLQLSF"
     gene            5414..6190
                     /locus_tag="PK742_08400"
     CDS             5414..6190
                     /locus_tag="PK742_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQU76651.1"
                     /translation="MHHLSSNLKYLRAARKLSQQELADLLEVGRTAIANYESGIANPG
                     HKTLLALVNIFGISLDELLYQDLQETGINRKEFQPVVVTVDKSGQENIVLVDTKAAAG
                     YPTRYLEPEYYKELPAFQLPGADYRNGTFRCFEVEGDSMNDTLQSGDYVIGRFCDNHY
                     KDVREGYVHVVVTGDQVLVKRVINKAEQESKLILLSDNDVYPPIEAGLDEVKEIWQVK
                     SKLSSYLPVKRNDLDRLIGDLTSEIRMLKQRVDDMQRGEA"
BASE COUNT         1721 a         1539 c         1718 g         1464 t
ORIGIN      
        1 cgatggcttc catacgggca gcatagttcg ggtcttccag cctcaaacgg gcatccactt
       61 ccatggcacc acattgacga taggcctcct gagccatcac tgaccccatg gttccagcca
      121 acagaaccgt aaacagaaat cttttcatgt tcttttttgg tttttgattg tgtgggttaa
      181 acaataatac ttttgtgtgg tctgcagggt tttgcaaaca ggtgcaacac ggcatctgca
      241 actacttaga gcatgctaaa ttagcttttg gttgcaatac cggaacacct ttttcatgac
      301 gctgttttcc agtgtcacat tagtgaaaac atttatcaac agcactgaca ggaaaggcca
      361 taatgagaag cgagaaccag atgaaaattt tttttaatcc cgtcacggat tggtcttttc
      421 atagattttt ttactattga taaaaatggg tattaaatga tccctttaaa tgacaaaaag
      481 ataaaaagga attctaaaaa ttactgaatt ggaagaaaaa tgggatgcgc tgaaagaggc
      541 tctatctgcc ggaatactgg tattggatgg cgccatgggt acgatgatcc agcgttatgg
      601 tctgaaggag gaagatttca gaggagaaag gtttgctgac tggccgacag atcttgccgg
      661 aaataatgat ctgttaagcc tgaccagacc ggatatcatt cggcagatcc ataccgaata
      721 cctggaagcc ggtgcagata tcatagagac caacaccttt aatgcgaaca gcatttcgca
      781 ggctgactat ggcatggaat ctctggcata tgagctgaat gcagtggctg cccggcttgc
      841 ccgtgaggca gttgaaaagc accggcagat atctcccgaa aggcgggtgt tcgttgccgg
      901 agccattggc cctaccaata aaaccctgtc tttatcgccc gatgtgaata atcccggatt
      961 ccgggcactg gattttgaca cccttgcagt tgcctacatg gaacagatca acggactgct
     1021 tgatggcgga gttgatctgc tgcttattga gaccatcttt gacaccctga atgccaaagc
     1081 agcgctgtca gcctgccagc aggtcatgga cattcgcggc gtaagcatcc ccatcatgtt
     1141 gtcgggaacc attacagatg ccagtggcag aacactcagc gggcaaaccc tggaggcatt
     1201 tctgtattcc gtttcgcacg caccgctttt aagcattggc ctgaactgtg ctctgggcgc
     1261 cagacaactt ttgccctatg taagagaact ggctgacaag accggattat atgtatcagc
     1321 ttatccaaat gcggggctgc ccaatgcatt tggagaatat gatgaaagtg cccacagcca
     1381 tggagaagac atccggcact atctggagga ggggctcctt aatatagtag gaggttgctg
     1441 cggcaccacc cctgatcaca tcagggagat agccaggctc gtaacgatgc actcccccag
     1501 aaaaccagca actcctgata agaagcctca attcagcggt ctggaggcac tgcctgtttt
     1561 tgccggaagc aatttcatca atatcggtga acgaaccaat gtggcaggat ccagagcctt
     1621 caagaaactg atcaaagaaa accggtttga ggaagcactt caggtggctc gtcagcaggc
     1681 agagaacggc gcccagatgc tggacgtaaa tatggacgat ggcatgatca acggggtcga
     1741 ggccatgacc acctttctgc atctcctcat ggcagaacct gacattgccc ggctacctgt
     1801 tgtggttgat tccagcaaat gggaagtgat cgaagccgga ctgaaatgcc tgcagggccg
     1861 aagcgtggtc aactccattt ctttaaagga aggcgaaacc gagtttttac gccaggcaaa
     1921 aattgccaaa agactgggtg ctgttgtcat agtcatggct tttgatgaag aaggacaggc
     1981 tacaactttt gaaagacgaa cagctatctg ctccagagca taccggttgc tgaccgagaa
     2041 agccggattc caacctgaag agatcgtatt tgatccgaac atccttacca tagccaccgg
     2101 catggaagaa cacaatgatt atggtgttga tttcattcgc acggtcaggt ggatcaaaga
     2161 aaaccttccg ggaacactgg tgagtggcgg ggtcagcaat atctcttttg cttttcaggg
     2221 caatgacctg gtcagagagg ccatgcatac cgtgttcctg tatcatgcca ttgcagcagg
     2281 tatggacatg ggcattgtca atgccgggat gataggcatt tatgaagaca ttgatctgcc
     2341 attgaaagaa cgactggaag atgtattgtt caacagaaga cccgatgcca ctgagaggct
     2401 ggtggcattt gcagcttcca tgcagcaggg tgcagagatc aagactgcgc agacagatgc
     2461 ctggagaaca gaagatctgg agagtcggtt aaggtttgca ctggtgcatg gcaatacaaa
     2521 ttttattgag gccgatactg aggaggctct ggaagttctg ggcaatccat tagctgttat
     2581 tgaaggtcct cttatggcgg gaatgaatgt ggttggtgat cttttcggca gcggaaagat
     2641 gttcctgcct caggtagtta aaagcgcacg tgtcatgaag aaggctgtag cctggctgga
     2701 accacacctg cttcgccaca aagaactgtc gggagcagac cagcgcagca gaaagaaaat
     2761 cctgcttgca acagtcaagg gagatgtaca tgatattggt aaaaatatcg tatctgttgt
     2821 actggcatgt aacaactatg atatagtaga tatgggagtc atggttccgg gagaaaagat
     2881 cctggaaaaa gcagtggaag aagaggtaga tatgatagga ctgagcgggc tcatcacgcc
     2941 atcactggac gaaatggtgc gggtggcacg ggcgatggag gagcggggca tgcaattacc
     3001 acttctcata ggaggtgcca caacatccag attgcatact gccgtgaaga tagcacctgt
     3061 gtacagtggt gtgaccgtgc atgttacaga tgcctccagg tgtgtgggtg tagccgcttc
     3121 actgaccggc gaacaggcat tggcataccg aaaagaaata gacaccacct atcaggaact
     3181 acgggaacaa cacgcacgta aaaacaacga ccacaacctg cttccaatca cagttgcccg
     3241 tcaaaacaaa tggtcatttg actggaattc ttacacacca ccacttcctc aaagacccgg
     3301 cacatacgtc ttcaaagaag aggaaataac cgtagatgcc atcagggaat acattgactg
     3361 gacaccattt tttcaaacct gggaactaag cggcagatat cctgaaattc tgaccgatga
     3421 aatcgtgggg gaagctgcag ggaaactatg ggaggatgcg caggtcatgc tggacaccat
     3481 acagcaacag caatggatca gtttgcaggc ggtgatccgg attgagcggg cctcttccac
     3541 cggcgatgat atagtagtga ccgacgaatt caaccagcaa acgatgttcc ataccattcg
     3601 gcagcagcag gtgaaagccg gcgggctgcc aaattatgcg ctggcagatt tcattgcacc
     3661 caaagacagc ggggtgaccg attacctggg tgggtttgct gtgtgcgcac actggaaaac
     3721 agaacaggtt attcgtgata tggaagctgc ccacgacgat tattcagcca tactggcaag
     3781 ggcactggcc gaccgtttgg cggaagcact ggcagaatat gttcaccaac aggtgagaag
     3841 aacttactgg gggtatgaga ccggagatgc gggaactacg gaagacctca tccgtgaaaa
     3901 gtaccagggc atcagacctg cgccgggtta tcctgcctgc ccggaccaca cagaaaagcg
     3961 caccttgctg gactggctga acgcaactga gcatacttcc atccgtctga ccgagaccct
     4021 ggccatgcac ccggcagcca gtgtgtgcgg ctggtatttc tcgcatccgg aaagcaggta
     4081 tttcaccact ggcaggatcg gtgatgacca gctggaagac ctgggaaaaa ggaaacagat
     4141 gccggcagca tcgctgcgca actggctggg catccggtaa ctggcaggcg ggtcagaaag
     4201 acagctgtaa ttgaccgggc cgtcggaaag cagacaggtc aagcgagacc ttctcccgat
     4261 cgccgaaata tttttcgcgg gtgatggtgt acatacgtcg gatggtttcc gcccactgtc
     4321 cttcgccctt cattctggtg ccaaacctgc tatcatttaa tttacctccg tgcaggtcgc
     4381 acacttgcga cagcacctta tctgccatac ccggaaaggt tttacgtatc cagtcttcga
     4441 agatgacaga gaccgcaccg ttcaggcgaa gcacaatgta acctgcatca gaagcaccgt
     4501 gtttggccgc ccgtctgatc acttccggga gctcatgatc gttcagaccg ggaatgaccg
     4561 gtgccaccag tacctgtacg gggataccgt ggctgctcag ggtttccatc acctccagtc
     4621 ttttcttata ggtagcggtg cggggctcca gtttttcccg ggtcttctca tggattccgt
     4681 tcatagagat catgacctgt accagattca gagctgccag ttgctgcagg atgtccagat
     4741 cacgcaggat cagactgttc ttggtaacaa tacccacagg attgcgaaac ttcaggcata
     4801 ccttcagcag ttctcgggtg atcttccact ttctttcagc cggctggtag cagtccgtat
     4861 taccggaaaa ggagaccggc gatggtaccc atttcggatc catgaaggtg gcctccagaa
     4921 gcctgggcgc ttcgggcttc accataatgc ggctttcaaa atccaggcct gcgctgaagc
     4981 cccagtactg gtgagaattg cgggcataac aatacacaca gccgtgctca cagccctggt
     5041 agggattgac actccagagc atacctacat cggggctatc caccttgttc aggatctttt
     5101 tgggaaattc ccggaaaaac tgggtatcgg gtgaagactg ctcatgctca tcaatacctt
     5161 cggggtaaag aacagccctg tgttgcgcat gaaagcggtt gtcgggattg aactgcgcac
     5221 cacgaccgcg gagagttgtt gcctgtatgc cttccatata agtcagaata gatgacaaat
     5281 tacgtcaata aaaatgacat tgtcaatgta aatgacattt tttgtcaaaa aaattgactt
     5341 ttttttcttc gggtattgca gatgtcagca agtgtcaata tttttgacat acaagtcaat
     5401 taaactgaca gtcatgcacc atctctcatc caacctcaaa tacctgcggg cagccaggaa
     5461 gctgagccag caggaactgg ccgatcttct tgaagtaggg cgcacggcca tagccaatta
     5521 tgaatcgggt attgccaatc cggggcacaa gaccctgttg gcactggtca acatcttcgg
     5581 catttcgctg gacgaattgc tgtaccagga cctgcaggag accggcatca accggaagga
     5641 attccagcct gtggtggtga cggtagataa gagcgggcag gaaaatattg tgttggtaga
     5701 taccaaggct gcagcgggtt atcctacacg gtatctggaa ccggagtatt ataaggagtt
     5761 acctgccttc cagctgcccg gagcagacta ccgcaacggc accttccgct gtttcgaggt
     5821 ggagggcgac agtatgaatg acaccctgca gagcggcgat tatgtgatcg ggcgtttctg
     5881 tgataaccac tataaagatg tgcgggaagg ctatgtgcat gtagtagtga ccggcgacca
     5941 ggtgctggta aagcgggtca tcaacaaggc ggaacaggag agcaagctca ttctgctcag
     6001 cgacaacgac gtgtacccgc ccattgaagc aggcctcgat gaagtgaagg agatctggca
     6061 ggtcaaaagc aaactgtctt cctatctgcc cgtgaaacgc aacgacctcg accggctcat
     6121 cggcgatctc acgtctgaga tccgcatgct caagcagcgg gtggatgata tgcaacgagg
     6181 agaagcctga gtgatgcctg ttgcaagaca tctgtaccgg accgggcaca accattcgcc
     6241 cccgctttac tgagaccata cggcctcttg cctttcagta ccccttacgg cggtagcggg
     6301 gaaggtgtgt cggaaaagag tcagcagttg gcggttggca gtgggcaaaa acaagtatcg
     6361 ggtagcgagt atcaggtact atctaccaat gaccaatgac cagcaactaa aaaccaaatg
     6421 tgagaggtga gaagtgaatg gt
//
LOCUS       DAOMAX010000148         6438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1916_length_6438_cov_4.543632, whole genome shotgun sequence.
ACCESSION   DAOMAX010000148 DAOMAX010000000
VERSION     DAOMAX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1916_length_6438_cov_4.543632"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PK742_08405"
     CDS             <1..1418
                     /locus_tag="PK742_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76652.1"
                     /translation="RKFWKYVGVFGIFTNVAVAAYYCYIESWTISYVFHSLTGTFNGM
                     TQGEVSGFYDKYLDVRSSTTGIPYEAVVFYVVCLILNTYILSRGLHGVEKAAKIGVPL
                     LLVFGGFLGIRSLTMGSSMATAEFPDANSIAGLNFLWKPAFDSLANPTVWLNAAGQIF
                     FTLSVGMGTIHCYAAYVKSKDDIALNAMSAGWMNEFVEVVLGSLIVIPLAAGFFGVDW
                     LKENAGFGMAFRTMPYMFEQWGGVLSALAGMMWFGLLFFAGITSSLAMGTPWMGFMRD
                     EFGYSRNKGAMSFGLIVLLMGLPTVLIYNFATNGTVNSFVFNEYDYWAGTVSLVVFAL
                     AETILFVWVFGLTKGWKEINDGGDIRVPDFYKPIIKWITPLLLLAVFIGSLITPEGND
                     WVNAFQNGWVLDNGSIIKTIANAGLKEQIATATDPAEISRLENQLLYTNAARVLLVLL
                     FAGISLTVYRAYAKRIKEGRA"
     gene            1421..1564
                     /locus_tag="PK742_08410"
     CDS             1421..1564
                     /locus_tag="PK742_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76653.1"
                     /translation="MDTSALVLMLVVQVAVTAITLYFFLKVLRTPPRAEPDSYDENDD
                     EPR"
     gene            1619..2740
                     /locus_tag="PK742_08415"
     CDS             1619..2740
                     /locus_tag="PK742_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQU76654.1"
                     /translation="MRVKPTRAFEFSSRERVSFWVLICILTCSLASIIWMHSRDSEVV
                     ILYEKEIPVLPVVDPQPEDISEPLTAPVDPNKADLNTLRRAGLSGKVASNIMRYREKG
                     GSFRSLKDMEKIYGMTEEMLDQASSWFVFPDAKPRTPDNRSGVGEPDERMTVVQPAER
                     FLFDPNTLDREGWIRLGVPEKAATNVIRYREKGGLFRKPEDIGKIYGMPDQLAAELQP
                     YIQIEAQPARADEKLHSEVSAVALNGNTDLNVADAATLIDCGINKNTAWDIINYRDRL
                     GGFYTASQLLEIKQLDSLDWQLLSSCVFIDAKQIRTMSINTAGYAELANHPYLSDRLA
                     RAIIQYRDSTGRYRSTDEMARAYRMSPAYLEKLKFYLRL"
     gene            2737..3270
                     /locus_tag="PK742_08420"
     CDS             2737..3270
                     /locus_tag="PK742_08420"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HQU76655.1"
                     /translation="MSVEQQIKQTLREVADFPKEGIVFRDLTPLLLHPELTEDILNAM
                     ILPFKEERIDAVCAIESRGFLYGMLLANKLNVPFLPVRKKGKLPGETLEYTYDLEYGS
                     ATVEVHLEDIQKGWNIMVHDDLLATGGTAAAATELMRMRGAQVVGFTFIVELGFLHGR
                     QRLEQYSDKIISLASFN"
     gene            3289..4428
                     /locus_tag="PK742_08425"
     CDS             3289..4428
                     /locus_tag="PK742_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQU76656.1"
                     /translation="MNFLLNEQVELVAATARDFAEKHIRPDMMMWDEEQIFPVDVLHK
                     AGELGLMGVLVPHEYGGAGLGYHEYMHVIMEIAAVCGSVGLSVAAHNSLCTGHMLQFG
                     NEEQKKKYLPKLATAEWIGAWGLTEPNTGSDAGNMQCVATRDGNDWIINGTKSWITHG
                     NSGNVAVVIARTGEPRTSRNCTAFIVERGTPGFSAGKKENKLGMRASETAEMIFDNCR
                     IADSQRLGEVGDGFVQAMKVLDGGRISIAALSLGIAKGAYKAALQYAKERKQFDKPIA
                     EFQGIAFKLADMATRIEAAELLTSQAADLKNRGEKVTRESAMAKYYASEVAVAVSTDA
                     VQIFGGYGYTKDYPAEKFYRDSKLCTIGEGTSEIQKLVIAREVLR"
     gene            4539..4730
                     /gene="rpsU"
                     /locus_tag="PK742_08430"
     CDS             4539..4730
                     /gene="rpsU"
                     /locus_tag="PK742_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQU76657.1"
                     /translation="MLIIDAREAESIDKALKNYKKKFEKAGILRELRRRQSFTKPSIE
                     RRTEILKAEYRQQMQSKED"
     gene            4775..5686
                     /locus_tag="PK742_08435"
     CDS             4775..5686
                     /locus_tag="PK742_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQU76658.1"
                     /translation="MMHLNGFFAYLQFEKRYSDHTIQAYRNDLAQLQDFLHGQGLDAG
                     DDLISLGYRQVRTWIMHMMADGMQPRSVHRKISSLRTYFRYLRKKGVISQNPMTKVVV
                     PRSGKPLPLFIDTAGMEVMFDNLVRQYPEQDPLDIFEKSRDLLILDILYKTGIRRAEL
                     LSLQERSFDRSSRNIRVVGKGNKERIVPLGEETLRLLEDYLAQRISLFGSGGALLLTR
                     TGKPAYPNMIYRLVRKSMEGATTLSRKSPHVLRHTFATHLSNNGAELNAVKELLGHAS
                     LASTQVYTHNSIDQLKEIYKRAHPKGS"
     gene            5711..6013
                     /gene="raiA"
                     /locus_tag="PK742_08440"
     CDS             5711..6013
                     /gene="raiA"
                     /locus_tag="PK742_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQU76659.1"
                     /translation="MQVNIQSIHFSADQKLKDYINAKLDKLTTFYDQIIQADVYLKLE
                     NEGAPIQDKVVEVRLHIPGNDLFAKETRMSFEEAADEVAENLRRQIKKQKEKISAK"
     gene            6121..6193
                     /locus_tag="PK742_08445"
     tRNA            6121..6193
                     /locus_tag="PK742_08445"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6154..6156,aa:Thr,seq:tgt)
     gene            6202..6284
                     /locus_tag="PK742_08450"
     tRNA            6202..6284
                     /locus_tag="PK742_08450"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6236..6238,aa:Tyr,seq:gta)
     gene            6365..6437
                     /locus_tag="PK742_08455"
     tRNA            6365..6437
                     /locus_tag="PK742_08455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6398..6400,aa:Gly,seq:tcc)
BASE COUNT         1710 a         1431 c         1667 g         1630 t
ORIGIN      
        1 agcggaagtt ctggaaatac gtgggggttt tcggcatttt taccaatgta gcggtggcag
       61 cttactattg ttacattgaa agctggacca tttcttacgt ctttcattca ctgacaggca
      121 ctttcaatgg catgacccag ggtgaagtgt ccggcttcta tgataaatat ctggatgtta
      181 gaagttctac taccggtatt ccgtatgaag cagtggtgtt ttatgtggta tgcctgatcc
      241 tgaataccta tattctgtca aggggcttgc acggagtgga aaaagctgcc aagatcgggg
      301 tgccgctgct gctggtattt ggcggattcc tgggtatcag gagtctgacc atgggatcca
      361 gtatggccac tgctgagttt ccggatgcaa actctattgc cggtctgaat ttcctgtgga
      421 aacccgcttt tgattctctt gccaatccca cagtctggct aaatgctgcc ggacagatat
      481 tcttcacctt gtctgttggt atgggtacga tacactgcta tgctgcctat gtgaaatcta
      541 aagatgatat tgcattgaat gcaatgagtg ccggctggat gaacgagttt gtggaagtag
      601 tgctgggctc actgatcgtt atcccattgg ctgcgggttt ctttggtgtt gactggctga
      661 aggaaaatgc aggatttggt atggctttcc gcaccatgcc ttatatgttt gaacaatggg
      721 gtggggtatt gtctgcactg gccggtatga tgtggttcgg tctgttgttc tttgcaggca
      781 tcacgtcgtc tttggccatg ggcactccct ggatgggatt catgcgtgat gaattcgggt
      841 atagccggaa taagggcgcc atgtcattcg gactgatcgt gctgctgatg gggctgccca
      901 cagtgcttat ctacaatttc gctacaaacg gtacggtcaa ttcttttgtc tttaatgaat
      961 atgattactg ggccggaacg gtatcactgg tggtatttgc cctggcagag accatcctgt
     1021 ttgtgtgggt tttcggactc accaaaggct ggaaagagat caatgacgga ggtgatatcc
     1081 gggtgcccga tttctataaa cctatcatca agtggatcac accactgttg ctgcttgccg
     1141 tattcatcgg ctcacttatc acccccgaag gaaatgactg ggtgaatgca tttcagaatg
     1201 gttgggtgct ggataatggt tccatcatca aaaccattgc caatgccggt ctgaaagaac
     1261 agatcgccac cgcaactgat ccggcagaga tctccaggct ggagaatcag ttgttgtaca
     1321 ccaatgcagc cagggtactg ctggtactct tgtttgccgg tatcagcctg accgtatatc
     1381 gggcgtatgc taaacgaata aaagaaggga gggcctgatc atggatactt cagctttggt
     1441 attgatgctg gtggtacagg ttgctgtaac tgccattact ttgtatttct tcctgaaggt
     1501 actacgcaca ccaccacggg cagagccgga ttcttacgac gagaacgacg acgagccccg
     1561 ttgatctgcc ggagtttagg cagccgttca tgtgacgggc tgttttgaac atccgtgtat
     1621 gcgtgtaaaa ccaacgagag cttttgagtt ctcttcccgt gaaagggtat cattctgggt
     1681 gcttatttgt atcctgacct gcagtcttgc aagtattatc tggatgcaca gcagagattc
     1741 agaagtggtg atcctgtatg aaaaggaaat ccctgttttg ccggtagttg acccgcaacc
     1801 ggaagacatt tcagagccgc tcacagcacc tgttgaccct aataaagcag atctgaatac
     1861 cttacgccgg gccggacttt ccggaaaagt agcgtccaac atcatgcgct atcgcgaaaa
     1921 aggcggatcc ttcaggtccc tgaaggatat ggaaaagatc tatggaatga cagaagaaat
     1981 gctggatcag gcatcttcct ggttcgtgtt tcctgatgcc aaaccccgta cccctgataa
     2041 caggtcaggt gttggagaac ctgatgaaag gatgactgta gtgcaaccgg cagaaaggtt
     2101 tttatttgat ccgaatacac tggataggga aggatggata cgcctgggtg tgccggagaa
     2161 agcagccaca aatgtaatca ggtaccggga gaagggaggg ctgttcagaa agcctgaaga
     2221 catcggtaag atatacggaa tgcccgatca gcttgcagca gaattacaac cctatataca
     2281 gatagaagct caacctgcaa gagcagatga aaaactgcat tcagaagtgt ctgctgtggc
     2341 actgaatgga aatactgacc tgaatgttgc agatgcagcc actctcatag attgtggtat
     2401 caataaaaac acagcctggg atattatcaa ctaccgcgat cgtctgggag gtttttatac
     2461 agcttcccag ctcctggaaa taaagcaact tgacagcctt gactggcagc tcctttcttc
     2521 ttgtgttttt attgatgcaa agcagatcag aaccatgtca atcaatacgg caggctatgc
     2581 tgaattggca aatcatccgt atctttccga ccgcctggcg agggctatta ttcagtaccg
     2641 tgattctacc ggaagataca gaagtacgga tgaaatggcc agggcctacc ggatgtcgcc
     2701 ggcctatctg gaaaaactga aattttacct gcgattatga gtgtggaaca gcagatcaag
     2761 cagactttga gagaagtggc cgattttcct aaggaaggta ttgtcttccg cgaccttact
     2821 ccgctgctgc ttcatccgga gctgaccgaa gatattctga atgccatgat cctgccattt
     2881 aaggaagaac gcattgatgc agtctgcgcc attgaaagcc gtggtttcct ctatggcatg
     2941 ctgctggcca acaagcttaa tgtgcctttt ctgccggtca gaaagaaagg aaagcttccc
     3001 ggcgaaacac tggagtacac ctatgatctg gagtacggtt ctgctactgt ggaagtgcac
     3061 ctggaagaca ttcaaaaagg ctggaatatt atggtacatg acgacctgct ggctaccggt
     3121 ggtactgctg cagcagccac tgaactcatg cgtatgcggg gtgcacaggt ggttggtttt
     3181 acctttatcg ttgagcttgg ttttcttcac ggcaggcaac gcctcgaaca atattctgat
     3241 aagatcattt ctttagcaag tttcaattaa tataacctta actcagagat gaatttcctc
     3301 ttgaatgaac aggtggaact cgttgcggca accgcacgtg attttgctga aaaacatata
     3361 cgaccggata tgatgatgtg ggatgaagaa cagatcttcc ccgttgatgt attgcataaa
     3421 gccggcgaac tgggcttgat gggggtgctc gttccccatg agtatggtgg tgcaggcctc
     3481 ggctaccatg agtatatgca cgtgatcatg gagatcgccg ctgtttgtgg ctccgttggt
     3541 ctttctgtgg cggcacataa ctctctctgt actggtcata tgctgcagtt cggaaatgaa
     3601 gagcagaaaa agaaatacct gcccaaactg gccactgcag agtggatcgg tgcctggggg
     3661 ctcaccgaac ctaatacagg atctgatgcc ggcaatatgc aatgtgtggc tacccgcgac
     3721 ggcaatgact ggatcatcaa tggcaccaag agctggatca cccatggcaa ttccggcaat
     3781 gtggctgtgg tcatcgcccg tacaggtgaa ccccgcacca gccgaaactg tacggccttc
     3841 atcgtggaaa gaggaacacc cggattttct gccgggaaaa aggaaaataa gctgggcatg
     3901 cgtgcttcag agaccgcaga gatgatcttt gataactgcc gtatagctga cagccaacgt
     3961 cttggtgaag taggtgatgg gttcgttcag gctatgaaag tactggatgg cggacgtata
     4021 tctattgcag cactcagcct cggaattgcc aaaggagcat ataaggctgc cttgcaatac
     4081 gcaaaagaga gaaaacaatt tgacaagcca attgctgagt ttcagggtat cgcattcaaa
     4141 ctggcagaca tggccacccg gattgaagcc gcagagctgc tgaccagtca ggctgccgat
     4201 cttaagaacc ggggtgaaaa agtgacccgc gaaagtgcta tggccaaata ctatgccagt
     4261 gaagttgcag tggcagtttc aactgatgcc gtccagatct ttggcgggta tggctataca
     4321 aaggactacc cggcagaaaa attctaccgg gattccaagc tctgtaccat tggcgaaggc
     4381 accagtgaga tccagaaatt ggtcatcgcc cgggaggtgc tgcgctgatc gcatgctctt
     4441 tgctctaaat cttatgtatc tgccaaaccg gctaattggc taattggaaa tctgatttcc
     4501 cttccctaac tttgccttcc caaattgaat atcaaggtat gttgatcatt gacgcaagag
     4561 aagctgaatc tattgacaag gccctcaaga attacaagaa gaaattcgag aaggccggta
     4621 ttctacgtga actgcgcagg cgccagtcgt tcacaaagcc ctctattgag cgcaggactg
     4681 agatcctgaa ggcagaatac cgccagcaga tgcagtctaa ggaagactga gaaactattt
     4741 ctcttttagc ccgttcaagt gtatatttaa tggaatgatg cacctgaacg ggttttttgc
     4801 gtacctgcaa ttcgagaaac gttacagtga ccatactatt caggcttacc ggaatgatct
     4861 ggcacagcta caggactttc tgcatgggca gggattggat gccggagatg acctgatttc
     4921 cttagggtat cgacaagtac gaacctggat catgcacatg atggcagatg gcatgcagcc
     4981 acgctcagtg cacagaaaga tatcctctct tcgaacctat ttccggtatc tgcggaagaa
     5041 aggggtcata tcacaaaatc cgatgacaaa agtcgtcgta cccagatcgg gtaagcccct
     5101 acctttattc atagatactg cgggtatgga agtgatgttt gataacctgg taagacaata
     5161 tcctgaacaa gaccctctgg atatttttga gaaaagcaga gacctgctga tactcgatat
     5221 cctctataag accgggatca gaagagcaga gttgctgagt ttgcaggaaa gatcctttga
     5281 cagatcttcc agaaatatac gtgtggtagg aaagggaaac aaagaacgga tcgtacctct
     5341 gggcgaagaa acactccggt tgctggaaga ttatctggct cagcgaataa gcctctttgg
     5401 gtccggaggg gcattgctgc ttaccaggac agggaagccg gcatatccca acatgatata
     5461 tcgtctggtg agaaaaagca tggaaggtgc taccacactt tccagaaaaa gcccccatgt
     5521 gctgcggcat acctttgcca cacacctgtc taacaatgga gcagaactca atgcggtgaa
     5581 agaattgctg ggtcatgcaa gcctggctag cacccaggtc tatacccata attccattga
     5641 tcaattaaaa gagatctata aaagagcgca cccgaaaggg agctgattgt tgaatgataa
     5701 aaaccaaacc atgcaagtaa acatccaatc aatccatttt tctgctgatc agaaacttaa
     5761 agactatatc aatgccaaac tggataagct gacaaccttc tacgatcaga tcatccaggc
     5821 agatgtctat ttgaaactgg aaaatgaagg agcacctata caagacaaag tggtggaagt
     5881 gcgcctgcat atccccggaa atgatctgtt tgccaaagaa acaagaatga gttttgaaga
     5941 ggctgcagat gaagttgcag agaacctccg gcgccagatt aaaaagcaaa aagaaaaaat
     6001 tagcgccaaa tagcaagaaa agtttgctgg tctcataaaa gacttcgtat atttgcgggc
     6061 atttttccgg aaaacgacgg gaaaatgctt tttagtcttt gacataatgc gagtcgagag
     6121 gccaatgtag ctcagttggt agagcagctg atttgtaatc agctggtcgg gggttcgagt
     6181 ccctccattg gctcgtctaa tggggagatt ccagagtggc caaatggggc agactgtaaa
     6241 tctgttggcg tacgccttcg gaggttcgaa tcctcctctc cccacacgga ctgactgact
     6301 gtgacctatt gagcaagagt tgttacgaac tcgacctcac tgtcagattg gtcctcgcat
     6361 acaggcggga gtagctcagt tggtagagca tcagccttcc aagctgaggg tcgcgggttc
     6421 gagtctcgtc tcccgctc
//
LOCUS       DAOMAX010000149         6437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1918_length_6437_cov_4.072078, whole genome shotgun sequence.
ACCESSION   DAOMAX010000149 DAOMAX010000000
VERSION     DAOMAX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_6437_cov_4.072078"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3460
                     /locus_tag="PK742_08460"
     CDS             <1..3460
                     /locus_tag="PK742_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76660.1"
                     /translation="TDDAVCTSYDRHWKVNRSLIDSFKTVYANASETSPISYDPTFRV
                     IFEWPGLGNEYARGKSQSDVLVINRPLAPFVDVNFDGIYQPQFGEYPDIDGDQAIWWV
                     YNDKGNIHTESGGDAIGIEIQAMAFAFSTNDEINDMTFYRYNVINFATTPLDSVYFGY
                     WVDSDLGFYNDDWVGCDTTLSLGMTFNGDAVDGPDIASYGENPPIFGTDFFQGPIKYI
                     YDGGSIVDSIRLGMAAFLYYNNDFSVIGNPEVAAHYYGYLSGSWKDGSPFTYGGNGYG
                     GTDPTPFMFPSDPSDPLGWSECTESNPPADRRMIQSSGPFRLEPGAANEVVIGAVWVR
                     PPISGGCLTTFDAIALADQKAQALFDADFDLIDGPDAPDMSIRELDREIILSLTNSTN
                     SNNNGEAYEETDPIINSIAAEDPEVTDTTYNFQGYKVYQLINGQVSPSEYSDPAKARL
                     IFQCDLKDGVSKLVNFKFDPLLGADVPELMVDGEDGGIRHTLQVTDDAFATENTKLVN
                     FQTYYFSVVAYAYNDYRPYDPVNPNSQKTPYLEGRNNIKVYTAIPHKTDPENNGTILN
                     AQYGDGPDITQIEGQGNGGYYLELVDESVNTIIANGIDDSPTYMGRNAPIDVMIYDPL
                     RVPNAEFEVTLYDSSVAAIGASTGYVLNRDSTWWIMKNLNTGVTVKSDLPIDFINEQI
                     IPEWGLSVTVKAVNPPGPDPVVSGVEWEENNGVIGSDITYANTSFQWLAGIPDVDGVP
                     FLDWIRSGVDESDLGGQDDNQAFELLAGGSIAPTGLVQINGTTRYAMPLRQILGLTIS
                     LPQLKLSNLTSVDIVFTSDRSKWSQCVVLNTGEEEFTYPYGEDKLDLRQKPSKDINGN
                     EVTTGEVGRSWFPGYAIEVETGRRLNILFGEDPFVNEDGNADDMLFNPSSRFFDGAGV
                     NPVFGGKHTVFVHRTDYDGCQGLMDSLTWVDGSRPSSTIYREIFKNMIYVYRPILATG
                     QELLSLEDGLIPTETKVSVRVTRRFINNIKNNENGGFPKYSFSTNGLAASEGNTDIAS
                     SALDLINVVPNPYYAYSGYENSPVDNRIKITNLPPNCTVSIYMMDGTLIRRFDRAVSV
                     DNSSGGVLSTKNDNLDTSLEWDLKNEKNVPIASGVYLIHIDAGALGERTLKWFGVTRP
                     IDLDTF"
     gene            3471..4541
                     /locus_tag="PK742_08465"
     CDS             3471..4541
                     /locus_tag="PK742_08465"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQU76661.1"
                     /translation="MRRFLKITGVVVAGALSVSTFAGNPDRAGEAGASELLINPWAQS
                     SGWYGLNIASVQGVEAMNLNVAGLSFINNTEIAFSRTDYLVGSDISINTFGLAQRVGE
                     GVLGLSLSTFSLGEVPVTTTQLPEGTGAVYTPRLSNFAVAYSRKFADYLSGGFTIRGI
                     SESIADARAFGVSMDVGIQYITGSAEHPEQIKMGVALRNIGSPMRFQGDGVTFSGQVP
                     EGTYSTTIAFRTENFELPSLLTIGLSYDFYFGSKHRLTAVGNFVSNSFYKDQFGGGVE
                     YGLKVKGEEMFQIRAGYRYENGIASSDPDLRSTVNTGVAAGVTFQTRLNNTGNSKVAI
                     SYSYRPSDPFDGTHSLGVRLGL"
     gene            4611..5084
                     /gene="greA"
                     /locus_tag="PK742_08470"
     CDS             4611..5084
                     /gene="greA"
                     /locus_tag="PK742_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQU76662.1"
                     /translation="MATINYMTEEGLDQLKIEIQTLKTKGRAEMAQMIKEAREKGDLS
                     ENAEYDAAKEAQGLLELKISQLEEILANARVMDTAKIDTSKVSVLTTVRVFHMGLKKE
                     IEYTLVTEKEADLKQNKISVSSPIGKGLLGRKVGEEVEIQVPAGKLGMKILDIRI"
     gene            5086..5481
                     /locus_tag="PK742_08475"
     CDS             5086..5481
                     /locus_tag="PK742_08475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQU76663.1"
                     /translation="MASIFSRIIAGEIPAHKVLENDSFFAFLDIMPLAPGHTLVVPKK
                     EVDYLFDLEEDLLAGILPFSRRIAAALEQVFTCERIGVSVIGLEVPHAHVHLIPLRNM
                     DDINFSRPKMKVSPEELADMAQKIRSALD"
     gene            complement(5462..6025)
                     /gene="ruvC"
                     /locus_tag="PK742_08480"
     CDS             complement(5462..6025)
                     /gene="ruvC"
                     /locus_tag="PK742_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQU76664.1"
                     /translation="MEKIILGIDPGTNILGYSLLRGVKNTINISACGIVRFSGKEDHF
                     VRINKIFNSIGELIHQYQPDTLAIESPFFGKNVQSMLKLGRAQGAAILAGMQHNLEVV
                     EYSPSRIKQAITGNGNASKEQVANMLNSLYKIDLTSVHLDETDAIAVAVCHYFEGNRP
                     AGSGKQFRNWKDFLENNPGRGVNPKQT"
     gene            6057..>6437
                     /locus_tag="PK742_08485"
     CDS             6057..>6437
                     /locus_tag="PK742_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HQU76665.1"
                     /translation="MKRPANNIWSVVIKVVILALLCWALYEQLISQRRLDDVVREFRS
                     GVAENGWNLVLVCILLMFANWGVEAFKWNLLINKLVHIRFRRAFQAVWTGVTLGLFTP
                     NRVGEYGGRILYLPAGFRISGVVSS"
BASE COUNT         1688 a         1362 c         1571 g         1816 t
ORIGIN      
        1 tacagatgat gctgtttgta cttcctatga ccgtcactgg aaagtgaaca ggagtttgat
       61 agacagtttc aaaactgttt acgcgaatgc cagtgaaacc agccctatat cttatgatcc
      121 tacattcagg gtgatctttg aatggccggg actcggtaat gaatatgcac gcggaaagtc
      181 tcagtcagat gtactggtga tcaaccgtcc gctggctccg tttgtagatg tgaatttcga
      241 tggtatctat caacctcagt ttggcgaata tcctgacatt gacggagatc aggccatctg
      301 gtgggtgtat aacgacaaag gaaacatcca taccgaatct ggtggtgatg ccattggtat
      361 tgagatccag gcgatggcct ttgcgttctc cacaaacgat gagatcaacg acatgacctt
      421 ctacaggtat aatgtcatca actttgccac cacgccactg gactcagttt attttggtta
      481 ctgggttgac tctgaccttg gtttctacaa cgatgactgg gtaggttgtg atactactct
      541 ttcactcggt atgacattca atggtgatgc cgttgacgga ccggatattg cttcatacgg
      601 tgagaatcct ccgatctttg gtactgactt tttccagggg cctatcaagt atatttatga
      661 tggtggttcc attgtggatt ctatccgcct tggtatggcc gccttcctgt attataacaa
      721 cgacttttct gtgatcggta acccggaagt tgccgctcac tattatggct atctgtcagg
      781 tagctggaaa gatggttctc catttactta tggaggaaac ggctacggtg gtacagatcc
      841 tactccgttc atgttcccca gtgatccttc agaccctctt ggctggagtg agtgtacaga
      901 atccaatcct ccggcagacc gtcgtatgat ccagtcttca ggcccgttcc gcctggaacc
      961 gggtgcagcc aacgaagtgg taattggtgc agtatgggtt cgtcctccga tctccggcgg
     1021 ctgccttacc acttttgatg caattgctct ggctgaccag aaggcacagg cattgttcga
     1081 tgcagatttt gatctcatcg atggtccgga tgcacctgat atgagtattc gcgagctgga
     1141 tcgtgagatc attttgtctc tgaccaactc caccaattcg aataacaacg gagaagcata
     1201 cgaagaaact gatcctatta taaacagtat tgctgcagaa gaccctgaag taaccgatac
     1261 cacttataat tttcagggtt ataaagttta tcagcttata aatggtcagg tgtctccttc
     1321 agaatattcc gatccggcta aggcccgttt gatcttccag tgtgatctga aggatggcgt
     1381 ttccaaactg gtcaacttca aatttgatcc attgctgggt gctgacgtac ctgaactgat
     1441 ggttgatgga gaagacggtg gtatcagaca cactcttcag gtaactgatg acgccttcgc
     1501 aactgaaaat actaagctgg tcaacttcca gacatattat ttctcagttg tggcctatgc
     1561 atacaatgat tacaggccct acgatccggt gaatccgaac tctcagaaaa caccgtatct
     1621 ggaaggacgt aataacatta aggtctatac agccatacct cacaagaccg atccggagaa
     1681 taatggaact atactgaatg ctcagtatgg tgatggcccc gacattacac aaattgaagg
     1741 ccagggtaat ggaggatatt atctcgagct ggtggatgaa tctgttaaca cgatcattgc
     1801 aaacggcata gatgattcac ctacctatat gggtcgtaac gcaccgattg atgtgatgat
     1861 ctatgacccg cttcgggttc caaacgctga gtttgaggtc actttgtatg actcatctgt
     1921 agcggcaatc ggtgcatcaa caggttatgt gctgaacaga gattctacct ggtggatcat
     1981 gaagaacctg aacaccggtg tgacagtcaa atctgacctg ccaattgatt ttatcaatga
     2041 gcagatcatt ccggaatggg gcttgtctgt tacagtgaaa gcagtgaatc ctccgggtcc
     2101 ggatcctgta gtttccggtg tggaatggga agaaaataat ggtgtgattg gttcagacat
     2161 cacctatgca aataccagtt ttcaatggct ggccggtatc cctgatgtgg atggtgtgcc
     2221 attcctggac tggatccgtt caggtgttga tgagtcagat cttggcggcc aggatgataa
     2281 tcaggcattt gaactcctag caggtggttc catagcacct accgggctgg tacagataaa
     2341 cggaacaaca aggtatgcta tgcccttgcg tcagatctta ggtttgacca tttctcttcc
     2401 gcaattgaaa ttgtctaacc tcaccagtgt ggatatcgta ttcacttcag atcgctccaa
     2461 gtggtctcag tgtgtggtgt taaatactgg agaagaggag tttacatatc catatggcga
     2521 agacaagctt gatttacgcc agaagccttc caaggacatc aatggaaatg aagttaccac
     2581 gggtgaggtg ggtcgttcct ggttcccggg ttatgctatt gaagttgaaa caggcagaag
     2641 gttgaatatt ctgtttggcg aagatccttt tgtgaatgaa gatggaaatg cagatgatat
     2701 gctcttcaac cccagctcca gattttttga tggtgctggt gtgaaccctg tatttggagg
     2761 gaagcataca gtatttgtac acagaaccga ttatgatggt tgtcagggtc ttatggactc
     2821 tcttacctgg gtagatggtt ctcgtccaag ttcaaccatc tatcgtgaga tcttcaaaaa
     2881 tatgatctac gtttacagac ctatactggc aacaggtcag gaattactca gtctggagga
     2941 cggattgatc ccaacggaga ccaaagtgag tgttcgtgta acacgacgct tcatcaataa
     3001 cattaagaac aatgagaatg gaggttttcc taaatattcc ttctcaacta atggacttgc
     3061 agcatcagaa ggtaatacag atatcgcttc atcggcactg gatctgatca atgtggtacc
     3121 gaacccttac tatgcctatt ccggctatga gaatagcccg gttgacaaca ggatcaagat
     3181 caccaatttg ccacccaatt gtactgtcag tatctatatg atggatggca cattgatccg
     3241 caggtttgat cgggccgttt cagtagataa cagttctgga ggtgtactct ccactaagaa
     3301 cgacaatctg gatacttctt tggaatggga tcttaagaat gagaagaatg ttcctattgc
     3361 cagcggtgtt tacctgatcc atattgatgc cggtgctctg ggtgagcgca cgttgaaatg
     3421 gttcggtgtt accagaccaa ttgaccttga caccttctaa actgataatg atgagaagat
     3481 ttttaaaaat taccggtgtt gtagtagcag gagctttatc tgtatcaacc ttcgccggaa
     3541 atccggacag ggcgggtgaa gcaggtgcct ctgaactcct gatcaatccg tgggctcaaa
     3601 gctcaggatg gtatggtctg aacattgcct ctgttcaagg agtagaagca atgaatctga
     3661 atgtagcagg cctgagtttt atcaataaca ccgaaattgc gttttcacgc acagattatc
     3721 tggtgggctc tgatatcagc atcaacactt ttggcctggc ccaacgtgta ggtgaaggtg
     3781 tgctggggct gagtctaagc acgttcagcc tgggcgaagt gccggttact accacacagc
     3841 tccccgaagg aacaggagcc gtatatacac ccagacttag caattttgct gtggcctaca
     3901 gccgcaaatt cgctgattat ctgtcaggtg gttttaccat tcgtggtatt tctgaaagca
     3961 ttgccgatgc cagagccttc ggagtcagta tggatgtggg tattcagtat attaccggtt
     4021 ctgctgaaca tcccgagcaa atcaaaatgg gtgtagctct ccgaaatatc ggatcaccca
     4081 tgcgtttcca gggtgatggt gtgaccttta gtggtcaggt gccagaaggt acttacagca
     4141 ctaccattgc ttttagaact gagaattttg agttgccatc attgcttacc attggcttgt
     4201 cttatgactt ctattttggt tccaagcatc gcctgacagc agttggtaac ttcgtttcca
     4261 actctttcta taaagatcag tttggaggag gtgttgagta cggcttaaaa gtaaaaggtg
     4321 aggaaatgtt ccagatcagg gccggttacc gttatgaaaa tggaattgcc agttcagacc
     4381 cggatctccg cagcaccgtt aatacgggag tggcagccgg agttaccttc cagacaagac
     4441 tgaataatac cggaaacagt aaagtagcga taagctattc ttaccgtcct tcagatcctt
     4501 ttgatggaac ccactctttg ggagtgcgtc tgggattgta attcattgtc taacaactta
     4561 tttggaaacg tttcttttga agaaacgttt ctttttttaa tgtatatgct atggcaacta
     4621 tcaattatat gacagaagaa ggtctggatc aattgaaaat tgagatccag acgcttaaga
     4681 caaaaggaag ggctgagatg gctcaaatga tcaaagaagc ccgtgaaaaa ggagacctga
     4741 gcgaaaatgc tgaatatgat gcggccaaag aagcacaagg cctcctggaa ctgaagatat
     4801 cacaattgga agagatactg gccaatgccc gtgttatgga cactgcaaaa attgacacca
     4861 gtaaggtttc tgtactgaca actgtcaggg tgttccatat gggtttaaag aaagagattg
     4921 aatacacatt ggttacagag aaagaggctg atctgaagca aaacaaaatt tctgttagct
     4981 ctccaattgg caagggtctc ctgggcagaa aggtaggaga ggaggtcgag atacaggtgc
     5041 ctgctggtaa gttgggaatg aagatacttg atatcaggat ctgatatggc ttcaatattc
     5101 agcaggatca tagcaggtga gattccggca cataaggtgc tggagaatga tagttttttt
     5161 gcttttctgg atatcatgcc tcttgccccc ggtcataccc tggtggtgcc aaagaaagaa
     5221 gttgattatc tctttgacct ggaagaagat cttcttgccg gaatcctgcc tttttccaga
     5281 agaattgcag ccgcactgga gcaggttttc acttgtgaac gtatcggtgt atctgtcatt
     5341 ggactggagg tgcctcacgc acatgtgcac ctgataccac tgcgcaatat ggatgatatc
     5401 aatttcagca ggcctaagat gaaggtgtct ccggaagaac tggcagatat ggctcagaag
     5461 atcaggtctg ctttggattg acacccctgc ctggattatt ttctaaaaaa tctttccagt
     5521 ttctgaactg cttgccagaa cccgccggcc tgtttccctc gaagtaatga cacactgcta
     5581 ctgctatggc atcggtctca tcaagatgca cagaagtcag atcaattttg tacaagctat
     5641 tcaacatgtt ggcaacctgc tctttactgg catttccatt gcctgtgatt gcctgtttga
     5701 tcctgcttgg actgtattct accacttcca gattatgttg cataccagcc agaatggctg
     5761 ccccctgcgc ccgccccagt ttcagcattg attgaacgtt ctttccaaag aaaggagact
     5821 caatagcaag ggtatctggt tggtattgat gaatcaattc tccgatgctg ttaaagatct
     5881 tattgatcct tacaaagtga tcttcttttc cggagaacct gactattccg caggcgctga
     5941 tattaatagt atttttaact ccccggagca gactgtatcc taaaatattt gtaccggggt
     6001 caatacccag aataattttc tccatgtgct gcgaatcctg gtaaatacct gtcaacgtga
     6061 aacgtccggc aaataatatt tggtctgttg taataaaggt agtgattctc gccctgctgt
     6121 gttgggcgct ttatgaacaa ttgatatcgc aaagaagact ggacgatgtt gtaagagaat
     6181 ttcgcagtgg tgtagctgag aatggctgga atctggtgtt ggtgtgtatc ctcctcatgt
     6241 tcgcaaactg gggcgtagag gcttttaaat ggaatctgct gatcaataag ttggtacata
     6301 tcagattccg aagggcattt caggctgtgt ggacaggggt tacactgggg ttattcaccc
     6361 caaatagggt tggtgagtac ggaggacgca tcctgtatct tcctgccggg tttcgtatct
     6421 caggagtggt cagttca
//
LOCUS       DAOMAX010000150         6383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1941_length_6383_cov_4.323009, whole genome shotgun sequence.
ACCESSION   DAOMAX010000150 DAOMAX010000000
VERSION     DAOMAX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_6383_cov_4.323009"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK742_08490"
     CDS             complement(<1..366)
                     /locus_tag="PK742_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQU76666.1"
                     /translation="MTPITIHWFRRDLRIHDNTALSLAHSEGNVLGLFIFDRNILDEL
                     EDRNDARVTFIHDALTRLEHDIRTEGGSLLVRFGDPLEVWKQLVDEFDIKAVFTNEDY
                     EPYAGRRDASVGSFLQLKGI"
     gene            complement(525..1364)
                     /locus_tag="PK742_08495"
     CDS             complement(525..1364)
                     /locus_tag="PK742_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQU76667.1"
                     /translation="MSQVWFNGQLQEAPFLVDTSNRAFRYGDGLFETVRVVYNRAPFL
                     HQHYIRFIEGMDMLRFDIPEHWSFTFLQKELDKLVRANDLQHARVRMTAWREGAGTHI
                     PEQHTASLLMEAFPLEEPYFQLNKEGLLIGVYPELRKEIHPLSSHKSANARPYVQAAI
                     FAAEQSWDDALILNTDGRPADAVQSSIFCWKNETLCTTADGDGGVDGVMRRVLLKAAK
                     QWGMPFSTEHMSIEELQAADEIFLTNTMQGIRWVGQWGDRVYDNRMAGILTGKLNEML
                     PLS"
     gene            complement(1367..2020)
                     /locus_tag="PK742_08500"
     CDS             complement(1367..2020)
                     /locus_tag="PK742_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HQU76668.1"
                     /translation="MLDKFGIAKRIAKELKDGYYVNLGIGIPTLVANYIPEGMNVILQ
                     SENGLLGMGPFPTEDKVDADIINAGKQTITTIPGSAFFDSAMSFGMIRAGKVDLTVLG
                     AMEVADNGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHTNKAGESKLLPACTLP
                     ITGLGVVKKIVTELGVFDVTPDGFQLLERAPGVTVDEIKAKTAGRLVVKGDIPEMMV"
     gene            complement(2032..2727)
                     /locus_tag="PK742_08505"
     CDS             complement(2032..2727)
                     /locus_tag="PK742_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQU76669.1"
                     /translation="MNKVVANADVAVADIHDGATLMLGGFGLSGIPENCIAALVRTGV
                     KGLTCISNNAGVDDFGIGLMLQGRQVKKMISSYVGENAEFERQLLSGELEVELIPQGT
                     LATRAMAAGYGMPAIYTPAGVGTEVADGKETRVFNGKTYLLEYAFDADFAIVKAWKGD
                     TMGNLIFRGTSRNFNPLMAMAGKITIAEVEELVPAGELDPNQIHTPGIYVHRIFQGTN
                     YEKRIEQRTVREN"
     gene            complement(2720..3094)
                     /locus_tag="PK742_08510"
     CDS             complement(2720..3094)
                     /locus_tag="PK742_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HQU76670.1"
                     /translation="MCFFLLFSLAFSACRPKSDLVFSEDMMQTIPDSVIGFDQMSAIL
                     AEVHLAEALVQETKVDSVRAHRDETLAGYYGFVLDKAGVDQDAFMRSYNWYVDHPLIM
                     NRLYQDVTQRLSILESKYKANE"
     gene            3143..3796
                     /locus_tag="PK742_08515"
     CDS             3143..3796
                     /locus_tag="PK742_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQU76671.1"
                     /translation="MNDNYRRISAAVAAAGATLVAVSKTKPVDAIREMYDAGQRDFGE
                     NKVQEMQEKAPQLPDDIRWHLIGHLQRNKVKYIASYVHMIHAVDSPELLAEIDKQAAR
                     VNRVIPVLLQVHIAEEETKFGFDAEELRAFLGDRPVYQNVRISGLMGMATFTDDMTQV
                     RKEFHGLKSLFDELKQTYFAGDEHFMHLSMGMSSDYEIALEEGSTLVRIGTLLFGAR"
     gene            3793..4821
                     /locus_tag="PK742_08520"
     CDS             3793..4821
                     /locus_tag="PK742_08520"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HQU76672.1"
                     /translation="MKRTIFLLLSTALLLQACGYTKAVKVAWDPANNPPIPDYSDSSA
                     WAALPDMKDSADHIPAMGLIDIQYTARADVFFIHPTSYFTRKHWNADIHDEKVNDHTD
                     EGTITHQASVFNGSCRVFAPRYRQTTYQAFFSLDNPEATEAFKLAYGDVKAAFQYYLS
                     HYNNGRPIIIASHSQGTVMAVWLLQEFFDGTPLQKQLVAAYITGMPVYNDDFTFIPVC
                     DSASQQQCYVSWRSFLYGHEPPEKYQIRNRENVVVVNPLTWTTDTGFVAPVHNIGGLG
                     KNAETIYPQVCGTEIHGDLLWVTKPDIPGKALLWMKNYHRADYNLYWMNIRDNVAVKV
                     NRYLEEHH"
     gene            complement(5037..5969)
                     /locus_tag="PK742_08525"
     CDS             complement(5037..5969)
                     /locus_tag="PK742_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HQU76673.1"
                     /translation="MEVQTDTWFMIINPVAGKGRAPKIWEQLSTYLSAANIDFQFAQT
                     QAPGHARQLAHKALASGGRKLMVIGGDGTANEVVNGIFHSGVDPQEVVLGMLSTGTGN
                     DWVKTIGSHGRLDTIPRNLRTCDCVAYDAGKLVYTKDGKTHERYFLNITGLGFDGAVT
                     RNLESGAGWLHNTRFRYWGALLQMLFKYRHTEVTYEIDGESFTYPTLSVAAGIGKYNG
                     GGMKQLPFADYNDGKLDLSIITNMSKLKMIISLPKLQSGSFVRMKQVRTFQAKSIHMH
                     SDMPLYVEADGEFLGEAPVHIGIIPDALRILRWK"
     gene            5994..>6383
                     /locus_tag="PK742_08530"
     CDS             5994..>6383
                     /locus_tag="PK742_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HQU76674.1"
                     /translation="MLTELRDDKWKALLDLLQHYYSALSDQSVKPQVVPGSVYASLPE
                     QLPDTPESFEKVLSDTRQLIMPALTHWQHPGFMALFPSNSSFPSLAGEVISSGVAINA
                     MMWDTSPAATELEQRMMEWLRDLCGLPS"
BASE COUNT         1553 a         1737 c         1499 g         1594 t
ORIGIN      
        1 aatacccttc aactggagga acgatccgac tgatgcatcc cgcctgccgg catatggttc
       61 atagtcttca ttggtaaaaa cggctttgat gtcaaactca tcaaccagct gcttccaaac
      121 ctccagcgga tcaccgaacc tgaccagcag agaaccgccc tccgttctga tgtcgtgctc
      181 cagtcgggtc agcgcatcat gaataaaggt gaccctggca tcgtttcggt cttccagttc
      241 gtccaggata ttcctgtcga aaatgaacaa tcccaacaca tttccttcac tgtgggccag
      301 gctgagagcg gtattatcat gtattctgag atcgcggcga aaccaatgga tggtaatggg
      361 tgtcatagct aagtactaaa ccgctgatac ttcagattgt tgaatgggtt ttacaattcc
      421 tttgagccct atgtgtctcc ttcgtgccat agtgttcaat attacaacac taagacgcaa
      481 agaaggcacg gagttcacag aggatcattc tatcagaatc tacgtcagga caacggcagc
      541 atctcattga gcttgccggt caggatacct gccatccggt tgtcatagac tcggtcaccc
      601 cactgcccca cccatcggat gccctgcatg gtattggtca gaaatatctc atcggcagcc
      661 tgcaattctt cgatggacat gtgttctgta ctgaacggca taccccactg tttcgccgct
      721 ttcagcagta cccgtcgcat cacaccatcc acgccaccat caccatcggc ggtggtacaa
      781 agtgtttcgt tcttccagca aaagatggac gactgcacgg catcggcagg acgtccgtcg
      841 gtattcagaa taagtgcatc atcccagctt tgctctgcgg caaatatcgc tgcctgtacg
      901 taaggtctgg cattggcaga tttatgggat gacagtggat ggatctcctt ccgcagttca
      961 ggatacacgc cgatgagcag accctctttg ttcagctgaa aataaggttc ttccagcgga
     1021 aaagcttcca ttagcaatga agctgtatgc tgttccggaa tgtgggtacc tgcaccttcc
     1081 cgccaggcag tcatgcgtac cctggcatgc tggaggtcgt tcgccctgac cagcttgtcc
     1141 agttccttct gcagaaaggt aaaagaccag tgttccggaa tatcgaaccg aagcatatcc
     1201 atgccttcaa taaagcgtat gtaatgctga tgcagaaaag gtgcacggtt gtacaccaca
     1261 cgaacggtct cgaacaaacc atcgccataa cgaaaagcac ggttggatgt atctaccaga
     1321 aaaggcgctt cctgcagctg cccgttgaac cagacctgcg acatgcttac accatcattt
     1381 cggggatgtc gcctttcacc accagtcggc cggcagtctt ggctttgatc tcatcaaccg
     1441 ttacgcccgg tgctctctcc agcagttgga aaccatcggg cgttacatcg aacactccca
     1501 gttcggtaac gatcttcttc accacaccaa gtccggtgat gggcaatgta caggcaggaa
     1561 gcagcttgct ttcgccggct ttattggtat gctgcatggc cacgatgata ttcttggcac
     1621 tggccacaag atccatcgca ccgcccatac cttttaccat ctttccgggt atcttccagt
     1681 tggcaatgtc gccattgtct gccacttcca tcgcacccag cacggtcaga tcaaccttgc
     1741 ccgcacggat catcccgaag ctcatggcac tatcaaaaaa tgccgagccg gggatcgtag
     1801 taatggtctg ctttccggca ttgatgatgt cggcgtcaac cttgtcttcc gtcggaaatg
     1861 gacccatacc aagcagcccg ttctcactct ggagaataac gttcatgcct tcgggaatat
     1921 agttggctac gagggtgggt atgccaatgc cgaggttgac gtaatatccg tccttcagtt
     1981 ctttcgcaat acgtttggcg atgccgaatt tatccaacat gatcgtctgt tttaattttc
     2041 ccgcaccgtg cgttgctcga tgcgcttttc ataattcgtt ccctggaaga tgcggtgcac
     2101 gtagatgccc ggtgtatgta tctgattcgg atccagttca cctgccggca ccagctcctc
     2161 cacttctgcg atggtgatct tgcctgccat ggccatgagc ggattgaaat tccggctggt
     2221 tccgcggaat atcagatttc ccatcgtatc tcccttccag gctttgacaa tggcaaaatc
     2281 ggcatcgaat gcatactcca gcagataggt cttgccgttg aacaccctgg tttcttttcc
     2341 gtcggctacc tctgttccaa cccctgcagg agtatagatg gcgggcatgc cataccctgc
     2401 tgccatcgca cgggtagcca gtgttccctg tggtatcagt tccacctcga gttcgccgct
     2461 cagcaactgc ctctcaaact cagcattctc gcccacatag ctggagatca tcttcttcac
     2521 ctgacgaccc tgcagcatca gaccgatacc aaaatcatcc actcctgcat tattggagat
     2581 gcaggtcaga cccttaactc ctgttcgcac cagtgcagct atacaattct ccgggatacc
     2641 gctcagtccg aaaccaccca gcatcagggt agcaccgtcg tgtatatcag caacggctac
     2701 atcggcattg gcaacaactt tattcattgg ctttgtattt actttccaga atactcaatc
     2761 gctgggtgac gtcctgatag agccggttca tgatgagcgg atggtcaaca taccagttgt
     2821 aactccgcat aaaagcatcc tgatccactc cagccttatc cagtacgaaa ccgtaatatc
     2881 cggccagggt ctcatcgcgg tgtgccctta cactgtccac ctttgtttcc tgcaccagag
     2941 cttctgccag atgcacttca gccaggatgg ccgacatctg atcgaagcca atgaccgagt
     3001 cgggaatggt ctgcatcatg tcctctgaaa acaccaggtc tgacttgggt ctgcaggccg
     3061 aaaatgccag cgaaaacagg aggaaaaagc acattactct cacaccttga ctatcttagc
     3121 tcgtcaaagg ctaaggtacg aaatgaacga taattaccgc cgtatcagcg cagcagtagc
     3181 ggccgccgga gctactctgg tggcagtgtc caagacgaaa ccggtggatg ccatcagaga
     3241 gatgtatgat gccggtcagc gggattttgg cgagaacaag gtgcaggaga tgcaggagaa
     3301 ggcgccgcaa cttccggacg atatccgctg gcatctgatc ggtcatctgc agcgcaacaa
     3361 ggtgaagtac attgcttcct atgtgcacat gatccatgcg gttgacagtc cggaactgct
     3421 ggcagagatc gataagcagg cggccagagt gaatcgtgtc attccggttc tgctgcaggt
     3481 tcatattgcg gaagaggaaa ccaaattcgg atttgatgca gaagaattac gtgcattcct
     3541 gggagacaga ccagtatatc aaaacgtacg catcagcgga ctgatgggga tggccacttt
     3601 taccgacgat atgacacagg tgcggaaaga atttcatggt ctgaagtcat tgttcgatga
     3661 gttgaagcaa acttattttg ccggcgacga acatttcatg cacttgtcga tgggcatgtc
     3721 gtccgattat gagatcgcac tggaagaagg gtccactttg gtgcgcatcg gaacactttt
     3781 attcggagca agatgaagag aacgattttc ctattgttgt ccaccgccct gctgctgcag
     3841 gcatgcggtt ataccaaggc agttaaagtg gcctgggatc ctgcaaacaa tccgcccata
     3901 cctgactaca gcgacagttc cgcctgggct gctttacccg atatgaaaga cagcgctgat
     3961 catattcctg caatgggatt gatcgacatt caatatacag ccagggcaga tgtgttcttc
     4021 atccacccca cctcctattt cacccgcaaa cactggaacg ccgacatcca cgatgaaaag
     4081 gtgaatgatc atactgatga aggtaccatt acccaccagg cttctgtatt caacggaagc
     4141 tgccgggtat ttgctccgcg gtacagacaa acaacttatc aggctttttt cagcctggac
     4201 aacccggagg caacagaggc attcaagctg gcatacggcg atgtaaaagc agcctttcag
     4261 tattatttgt cgcactacaa caacggccgc cccatcatca ttgccagtca cagccaggga
     4321 accgtcatgg cggtctggct cctgcaggaa ttctttgacg gcactccttt gcagaaacaa
     4381 ctggtggcag cctatatcac aggcatgccc gtgtataatg atgacttcac attcattcct
     4441 gtgtgcgaca gtgcttcgca gcaacaatgt tatgtcagct ggagaagttt tctgtacgga
     4501 catgaaccgc ccgagaaata ccagatcaga aacagggaaa atgtggtggt ggtgaatccg
     4561 cttacatgga ccacagatac cgggtttgtg gcacctgtac acaatattgg cggattgggg
     4621 aaaaacgctg agaccatcta tccgcaggta tgtggcacag agatccacgg agatctgctg
     4681 tgggtcacca aaccggatat tccgggcaag gctttgttat ggatgaagaa ctatcaccgt
     4741 gctgattaca atttgtactg gatgaatatc cgcgataatg tggcggtcaa agtgaaccgg
     4801 tatctggaag agcatcattg atgatgaaag ccggatccca gaataactca gatgaactga
     4861 tcacactgat ctgatcactc cgtgatcgca gacatatgta ttggtagttg gttattagtt
     4921 attggtggtt ggtcattagt ggtccttttt tcactactca ctcaccaccc ccactcccac
     4981 tcccactcac cactccccac tcgccactct cactcaccac tctcactcac cactccctat
     5041 ttccaccgta agatcctcaa cgcatccggt atgatgccaa tatgcaccgg agcttcgccc
     5101 aggaattctc catctgcttc tacatacaat ggcatgtcgc tgtgcatgtg aatggacttc
     5161 gcctggaatg ttctgacctg tttcattctg acaaatgaac cactttgcag tttcggaagg
     5221 gagatgatca ttttcagctt gctcatattg gtgatgatgc tcagatccag ttttccgtca
     5281 ttgtaatcgg caaaaggaag ctgtttcatt ccaccaccat tgtatttacc gatacctgcg
     5341 gctactgaca aggttggata ggtgaaactt tcgccatcga tctcataggt cacctctgta
     5401 tgccggtatt tgaacagcat ctgcaacagt gcaccccaat agcgaaaccg tgtattatgc
     5461 agccagccgg cacccgattc caggtttcgg gtaacggcgc catcaaagcc cagaccggtg
     5521 atattgagaa aatacctttc atgtgttttc ccatctttgg tatagacaag ttttcctgca
     5581 tcatacgcca cacaatcgca ggtacgaaga ttgcgtggaa tagtgtccag gcgaccatgt
     5641 gagccgatcg tcttcaccca gtcattcccc gtacctgttg acaacattcc caataccact
     5701 tcctgaggat ctacaccgct gtgaaaaatg ccattcacca cttcattggc agtaccatct
     5761 ccgccaatga ccatcagttt tcttccccct gaagccagtg ctttatgtgc cagttgccgt
     5821 gcatgaccgg gagcctgtgt ctgtgcaaac tgaaagtcta tatttgcagc agacagatag
     5881 gtgctgagct gttcccatat tttcggtgcc ctgcctttac cggccaccgg attgatgatc
     5941 atgaaccagg tgtctgtctg tacttccatg cctgaaggga ctaaagatat gttatgctga
     6001 cagaactccg tgacgacaaa tggaaagcct tgctggacct gttgcaacac tactactctg
     6061 ccctcagtga tcagtccgtc aaacctcagg tggtacccgg atccgtgtat gcatctctgc
     6121 ccgaacagct gcctgacaca ccggaatctt ttgaaaaagt tctatcagat acccgtcagc
     6181 ttataatgcc ggctctcacc cactggcagc atccggggtt catggcactc tttccatcca
     6241 acagcagttt cccgtcactg gcgggtgagg tcatatcatc cggagtagct atcaacgcca
     6301 tgatgtggga tacctcgccc gctgctaccg aactggaaca gcggatgatg gaatggcttc
     6361 gagatctgtg cggattgccc tcc
//
LOCUS       DAOMAX010000151         6352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1956_length_6352_cov_4.538987, whole genome shotgun sequence.
ACCESSION   DAOMAX010000151 DAOMAX010000000
VERSION     DAOMAX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_6352_cov_4.538987"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..4401)
                     /gene="rpoC"
                     /locus_tag="PK742_08535"
     CDS             complement(79..4401)
                     /gene="rpoC"
                     /locus_tag="PK742_08535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQU76675.1"
                     /translation="MPFKKDLKIKSDFTKIIIGLSSPDTILERSYGEVLKPETINYRT
                     YKPERDGLFCERIFGPIKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGMSSKKLDSVIYYERFCVIQPGPAAEGITINGELKK
                     YDTHDLITEEEYFDILDALGQENQMLDDHDPNKFIADMGAPAVHTMLSRINLDEMAAA
                     LRHQANTETSQQRKAEALKRLSVVESFREANEHLENKPEWMIVQYLPVIPPELRPLVP
                     LDGGRFASSDLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRK
                     SNAVKAEGGRALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPK
                     DMAAELFKPFIIRKLIERGIVKTVKSAKKLVDKKEAVVWDILENILKGHPVLLNRAPT
                     LHRLSIQAFQPKLIEGKAIQLHPLVCSAFNADFDGDQMAVHVPLSNAAILEAQMLMLS
                     AHNILNPQSGNPMTVPSQDMVLGLYYISKGKKSTKEEPIRGEGKVFYSAEEVIIAYNE
                     KKIDLHANIRCRVQVLKDEQMDWQIIETTVGRVIFNEVVPAQVGFINLLLTKKAIGKV
                     IGEIIKTTDVPTTAKFLDDIKDLGFRWAFRGGLSFNIGDLVEVEDKQKLVERAQGEVD
                     EVIYNYMNGFITDKERYNQIIDIWSRLNTRLTGELINQLTNDKQGFNSVFMMLDSGAR
                     GSKEQIRQLAAMRGLMAKPRKSGNTGGGEIIENPILSNLKGGLSVLEYFISTHGARKG
                     LADTALKTADAGYLTRRLVDVAQDVVITEEDCGTLRGIAVYALKDNEDIVEPLSERII
                     GRTSLHDVYDPITDELIINAGDLITAKEATRIDETAIEMVEIRSVLACESKRGVCVKC
                     YGTNMTNNRMAQMGDAVGIVAAQSIGEPGTQLTLRTFHVGGVAGSIDTESKLLAKFDG
                     KVEFDGLRFVDSENEEGKKVRVVLGRTGELRIVDPKTGKLFITNNVPYGSNLLVKEGD
                     VISKGDVLCSWDPFNAVIVSEVGGKVDYEDIIDGITFREETDEQTGHREKVIIDTRDK
                     KKIPTINLLDKKGNIIGTHNLPVGAHIMPEKADDIRPGQIIAKIPRALGKVKDITGGL
                     PRVTELFEARNPSNPAVVAEIDGVASYGKIKRGNREVIIENKDGEVRKYLVPLSKQIL
                     IQENDYVYAGMPLSDGAITPGDILSIKGPYALQEYIVNEIQEVYRLQGVNINDKHIEV
                     IVRQMMRKMTITDPGDTRFLEGQLVDKTDFLEENDRIFDKKVITEAGDSSNLRAGQIV
                     TLRQVREENSLLKRADKTPVEYRDATPATATPTLQGITKSSLGTTSWISAASFQETTK
                     VLSQASISARIDTLAGLKENVIVGHKIPAGTGLREFDQLIIGSKDELEAMMVHEEEVT
                     SESN"
     gene            complement(4438..>6352)
                     /gene="rpoB"
                     /locus_tag="PK742_08540"
     CDS             complement(4438..>6352)
                     /gene="rpoB"
                     /locus_tag="PK742_08540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQU76676.1"
                     /translation="FLEHDDANRALMGSNMQRQAVPLLRPQAPVVGTGLEQKVAADSK
                     MLINAEGNGVVEYVDANEIHIRYDRDEKSRLLSFEEDKKVYRLRKFKKTNQSTCINIK
                     PIVSKGDKVVAGQVLCEGYATDKGELALGVNLKVAFMPWKGYNFEDAIVISERIVKED
                     IFTSIHIEEYELEVRDTKLGEEELTADIPNVSEEMTKDLDANGIIRIGANIKEGDIMI
                     GKITPKGETDPTPEEKLLRAIFGDKAGDVKDASLKAPPSLEGTVIDKKLFARAKKDKN
                     AKTREKIALEKLDKDHERVLAELKEILVAKLLVLLKDKKSAGIRTTLNEEVVPAKTKF
                     TQKMLEGIHYNDINPYGWTGEAPVDDDVRMVLHNYGIKVNEELGRYKREKFNISVGDE
                     LPNGVLKLAKVFLAKKRKLKVGDKLAGRHGNKGIVARIVREEDMPFLEDGTPVDIVLN
                     PLGVPSRMNLGQIYETVLGWAGEKLGVKYATPIFDGANLGEISDQIKEAGLPELGKTY
                     LYDGETGERFHQQATVGVIYMLKLNHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQR
                     FGEMEVWALEAYGASNILQELLTVKSDDIIGRAKTYESIVKGENLPTPNIPESFNVLI
                     HELRGLTLDLKFE"
BASE COUNT         1424 a         1686 c         1455 g         1787 t
ORIGIN      
        1 atgctgctta aaaaactgca caaaaaaaaa ccggaaagat cactttccgg ttttatagat
       61 tgctttacat gataactatc aattactctc gcttgttacc tcttcctcgt gcaccatcat
      121 ggcttccagt tcatctttgg atccgatgat gagttgatcg aactctcgaa gtccggtacc
      181 tgcaggtatc ttatgaccta caatcacatt ctctttcaga ccagccagcg tgtctatacg
      241 ggctgaaatg gaggcctgac tgagtacctt ggtcgtttcc tggaaggaag ctgcagatat
      301 ccagctggta gtgcccagag agctcttcgt aataccctga agagttggcg tagcagtagc
      361 cggtgtagca tcgcggtatt caaccggtgt tttgtcagca cgtttcagca agctgttctc
      421 ttcacgaacc tgacgaaggg taacgatctg accagcacgc aggttggaac tgtcgccggc
      481 ttcagtgata acctttttgt caaagatccg gtcgttctct tcaaggaagt cagtcttatc
      541 aaccagctgc ccttccagga acctggtatc acccggatca gtgatggtca tcttacgcat
      601 catctggcga acgatcacct caatatgctt atcgttgatg ttcacaccct ggagacgata
      661 tacttcctga atttcattca caatgtattc ctgcagtgca taaggtcctt taatagacag
      721 aatatcaccc ggagtgatgg ctccatcact taatggcata ccggcataca catagtcgtt
      781 ctcctgaatg aggatctgct tgctcagcgg caccaggtac ttgcgaactt ctccatcctt
      841 attctcgata atgacctcac ggttaccacg cttgatctta ccgtagcttg ccacaccatc
      901 gatctctgcc acaactgcag gattgcttgg gtttcgggcc tcaaagagtt ccgttaccct
      961 gggcagacca cccgtgatgt ctttcacttt accaagagct ctcggaatct tggcaatgat
     1021 ctgacccgga cgaatgtcat ctgctttttc aggcatgatg tgggcaccca ccggaaggtt
     1081 gtgtgttccg atgatattac ctttcttatc aagcaggttg atggtcggga tcttcttctt
     1141 atcgcgggta tcaatgatca ccttttctct gtgaccggtt tgctcatcgg tttcttcgcg
     1201 gaaggtgata ccatcaatga tatcttcata atctacttta ccacctactt cagatacgat
     1261 gacggcattg aacggatccc agctgcagag tacatctcct ttgctgataa cgtctccttc
     1321 tttcaccagc aggttagaac cgtaaggcac gttgttggtg atgaagagtt tacctgtttt
     1381 cggatctacg atgcgcagtt cgccggtacg tcccagtacc acacgaactt tcttgccttc
     1441 ctcgttttca gaatctacga aacgcagacc atcaaactca actttaccgt caaacttggc
     1501 cagcaacttg gattctgtat caatacttcc ggccacacca cccacgtgga aggtacgcag
     1561 tgtcagctgt gtacccggct caccgataga ctgagcagct acaattccca ctgcatcacc
     1621 catctgggcc atccggttgt tggtcatgtt ggtaccgtag cacttcacac aaactccacg
     1681 cttggattcg caggccagta ccgagcggat ctccaccatt tcaatagcgg tctcatcgat
     1741 acgggttgct tcttttgcag tgatcagatc acctgcattt atgatcagtt catcggttat
     1801 aggatcgtac acgtcatgca gagaggtacg accaatgatc cgttcgctca gtggttctac
     1861 gatatcctca ttgtctttca gcgcataaac tgcaatacct ctcagggtac cgcaatcctc
     1921 ttccgtaatg accacatcct gggctacatc caccagacga cgggtcagat aacctgcatc
     1981 ggctgttttc agtgccgtat cggccagacc cttacgggca ccgtgtgtag agataaagta
     2041 ttcaagtacc gacaaaccac ctttcaggtt agagaggatc gggttttcga tgatctctcc
     2101 accgccggta ttgcctgatt tccttggctt ggccatcaga ccacgcatgg cagcaagctg
     2161 ccggatctgt tctttggaac ccctggcgcc actatccagc atcatgaaca cagagttgaa
     2221 gccctgtttg tcatttgtga gttgattgat cagctcgccg gtcagacgtg tgttcagcct
     2281 tgaccagata tcaatgatct ggttatagcg ttccttatct gtgatgaaac cgttcatgta
     2341 gttgtagatc acttcatcta cttctccctg agcacgttcc accagttttt gcttgtcttc
     2401 tacttctacc aggtcaccga tattgaatga cagacctccg cggaatgccc aacggaatcc
     2461 gagatccttg atatcatcca ggaacttggc agtggtaggc acatcggtgg ttttaatgat
     2521 ctcaccgatc actttaccga tagccttctt ggttagcagc aggttgatga atcctacctg
     2581 agcaggaacc acttcgttga agatgacccg tcctacagtg gtctcaatga tctgccagtc
     2641 catctgctca tctttgagca cttgaacacg acagcggata ttcgcatgca gatcgatctt
     2701 cttttcgttg tatgcgatga tgacctcttc ggcagaatag aacactttgc cttcaccgcg
     2761 aatgggttct tccttggtgc tcttctttcc tttagagata taataaagac ccagaaccat
     2821 atcctgagaa ggtaccgtca tcgggttacc gctctgcggg ttcaggatat tgtgggcaga
     2881 caacatcagc atctgcgctt ccaggatagc ggcattgctc aacggcacgt gcacagccat
     2941 ctggtcaccg tcaaagtcgg cgttgaatgc agaacacacc agtgggtgca gctgaatagc
     3001 cttgccttct attagtttcg gctggaaagc ctgaatagac aaacggtgca gtgttggcgc
     3061 cctgttcagc agaacggggt gacctttcag gatattttcg agaatatccc atacaacggc
     3121 ttctttcttg tcaaccagtt tcttggcaga cttgaccgtt tttacgatac ccctttcgat
     3181 cagtttacga atgatgaacg gcttaaagag ttcagctgcc atatctttcg gcagtccgca
     3241 ctcatgcagt ttcaattcgg gacctaccac gatcacagaa cgaccggaat agtcaacccg
     3301 cttaccgagc aggttctgac ggaagcggcc ctgcttacct ttcagtacat cgctgagtga
     3361 tttcagtgca cgtccacctt ctgcttttac agcgtttgat ttccgactgt tgtcgaacaa
     3421 ggagtcaaca gcttcctgca gcatacgctt ttcgttacgc aggatcactt caggagcctt
     3481 gatctcgatc agtcgcttca gacggttgtt gcggatgatg acacggcgat acaggtcgtt
     3541 cagatcagag ctggcaaaac gaccgccatc cagtggcaca agcgggcgca gttccggcgg
     3601 aatgacgggc agatactgta cgatcatcca ttccggtttg ttttccaggt gctcattcgc
     3661 ttcgcggaaa ctttctacca cactcaggcg cttcaaggct tctgccttac gctgctgaga
     3721 ggtttcggta tttgcctggt ggcgaagagc agctgccatt tcgtccaggt tgatccggct
     3781 cagcatggta tgcaccgcag gtgctcccat gtcagcaatg aacttgttcg gatcgtggtc
     3841 atccagcatc tggttctcct gacccagagc atccaggatg tcaaaatatt cttcttccgt
     3901 gatcagatca tgggtgtcat acttcttcag ttcaccattg atggtgatac cttctgcagc
     3961 gggtcccggc tggatcacac agaaacgctc gtaataaatt acggagtcca gtttcttgga
     4021 agacatacca agcagatatc cgatcttgtt gggcagtgat ttgaagtacc agatatgtac
     4081 aacaggtaca acaagtttga tatggcccat gcgttcgcgg cgaacctttt tctcggtcac
     4141 ttccacaccg caacgatcgc acacaatgcc tttgtaacgg atacgtttat acttaccgca
     4201 ataacattcg tagtccttga tagggccgaa aatgcgttcg cagaaaagtc cgtcacgttc
     4261 cggcttatag gtccggtaat taatggtttc cggcttcagt acttcaccat aggaacgttc
     4321 cagaatggta tctggagacg aaagaccaat gatgatcttc gtgaagtcag atttgatctt
     4381 gagatccttt ttaaaaggca tatgctgctc tcctttttat tgcgttaaaa aaacggatca
     4441 ctcaaatttc agatccaggg tcagacccct gagttcgtgg atcaacacgt tgaacgattc
     4501 aggaatgttc ggcgtgggca ggttttctcc tttcacaata gactcgtagg tcttagctcg
     4561 accgatgata tcatcgctct tcacggtaag aagttcctgc aggatgttgg aagcaccgta
     4621 ggcttccagc gcccatactt ccatttcacc gaaacgctga ccaccgaact gcgccttacc
     4681 tcccagcggc tgctgggtga tgagggagta tggtccgatg gagcgggcat gcatcttatc
     4741 atccaccatg tggttgagtt tcagcatgta gatcacccca actgttgcct gctggtggaa
     4801 gcgttcacct gtttctccgt catagagata ggtcttaccg agttccggca gaccagcctc
     4861 tttgatctgg tcagatattt cgcccaggtt ggcaccgtcg aagatcgggg tggcatattt
     4921 gacacccagt ttctctccgg cccatccgag aacagtttca tagatctgcc cgaggttcat
     4981 acgggaaggc acacccaatg ggttcagcac gatatctacc ggtgtaccgt cttcgaggaa
     5041 aggcatgtcc tcttcccgga cgatacgggc aacgataccc ttgtttccgt ggcgaccggc
     5101 cagcttatca ccgaccttca gcttgcgctt cttcgccaga aataccttgg ccagtttcaa
     5161 taccccgttt ggcagttcat cgcccacgct gatattgaat ttttcccgct tgtagcgacc
     5221 cagttcttca ttcaccttga tgccgtagtt atgcagcacc attctcacat catcatccac
     5281 aggtgcttca ccggtccagc cataaggatt gatatcatta tagtggatac cttccagcat
     5341 tttctgtgtg aacttggtct tggctggtac cacctcttca ttcagtgtgg tgcggatacc
     5401 tgcagacttc ttgtctttca gcaataccag cagcttggct accaggatct ccttcagttc
     5461 agccagtacc ctttcatgat ctttgtcgag tttctcgagt gcgatctttt cgcgggtctt
     5521 ggcattctta tctttcttgg cacgggcaaa cagttttttg tctatgaccg taccttccag
     5581 tgaaggaggt gctttcagag atgcatcttt cacatcgccg gctttgtcgc caaagatggc
     5641 ccggagcagt ttttcttccg gtgtcgggtc ggtctctcct ttcggggtga tcttaccgat
     5701 catgatgtcg ccttccttga tattggcacc gatacgaatg ataccgttgg catcaaggtc
     5761 tttggtcatc tcttccgata cgttcggaat atctgctgtc agttcttctt cacccagctt
     5821 ggtgtcacgt acttccagtt catattcttc aatatggatg gaggtgaaga tatcttcctt
     5881 cacgatcctt tcagagatca cgatggcatc ttcaaagttg taccctttcc atggcatgaa
     5941 agccactttg aggttgacac ccagagccag ttcaccttta tcagtggcat agccttcgca
     6001 cagtacctgt ccggccacga ccttatcgcc tttggaaacg ataggcttga tattgataca
     6061 ggtgctctgg ttggtctttt tgaacttacg cagtctgtaa actttcttgt cttcctcaaa
     6121 gctcagcagc ctgctcttct catcacggtc atagcggatg tggatctcat tggcatccac
     6181 atattctact acaccattgc cttctgcgtt gatgagcatc ttagagtcgg cggcaacttt
     6241 ctgttccaga ccggtgccta ccacaggcgc ctgcgggcgc agcagcggaa cggcctgacg
     6301 ctgcatgttc gagcccatga gcgcacggtt ggcatcgtcg tgttcgagga ac
//
LOCUS       DAOMAX010000152         6302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1979_length_6302_cov_4.021770, whole genome shotgun sequence.
ACCESSION   DAOMAX010000152 DAOMAX010000000
VERSION     DAOMAX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_6302_cov_4.021770"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PK742_08545"
     CDS             complement(<1..1243)
                     /locus_tag="PK742_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQU76677.1"
                     /translation="MKTRHKLSALAVAMLLGGATAFAQYCTPVYTTGTSDNDRIDGVV
                     LGDISNTASGGEITDIGYSDYTDLSTDLSNGATYTLSVTNGPSWSTTITAWIDFNQDE
                     VFDTDEVLGNLSLSAAATGDITFTVPVTALAGETRMRVRGVYPSGLGTPLDPCSSQTY
                     GEAEDYTVNLSGPLENNLTVTGIPAPASGADLGTVNVTVSIWNSGANDASGFTISYNV
                     DGGLITSEVYGGTLAAGESVDFTFGDDFTFSDYGCYDFLAWIDWADDSFDGDDSYSKT
                     VCNAGPVTGTGAWYVNSNTTGEPWGSTSNTDAMDAVFGAGGWSMEHYETADPFVLFSS
                     ENCFVFLEGSDAHANELETFLDANMTTIESWVSGGGRLLLNAAPNEGDGMSFGFDGTS
                     LIYSYFTGTGEAADPGHPIFAG"
     gene            complement(1327..3261)
                     /locus_tag="PK742_08550"
     CDS             complement(1327..3261)
                     /locus_tag="PK742_08550"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQU76678.1"
                     /translation="MKRSLLRWSGVAALALFIGTGSLSAQTAFSIGDSVGVNTNTTYP
                     TPFGDWYKTQRVQYLYPASELSAMGMGAGDITELAWYCNAPVIATDIPGLTEGYTIKM
                     MMTATTDLGAAWEEGATIVWGPMDFTPTTSAMNNFVLDVPFTWDGTSNIVIEICGGSS
                     AGTYTDNGVVAYATTAYNSSRTFRSDTELSPCTYSAAATTGTNPTRRPVTIFYGAVGD
                     ECTGTPDVSAAVSSAESVCAADVFTLSVDAITGFGISYQWQSSADGISYSDIAGATAS
                     SYDATQSEATYYQCVVTCAGSGESGTSASVWVGQNAATDCYCIPDYTSFLSGDYVDDF
                     SVADISNFDTGESGDNYNDFTYLSTDLTIGAEYEVFVDCGPSWDQGIAVYIDMNVDGT
                     FSADERLGCTLVDAGTGGATYTIMVPLGTAPGTTRMRVLADYADDCTSSMDQMAACEI
                     SSSFGETEDYTVNLVDGEACNSVTGLSADAGSTSATLSWDDMGATKYRFAYGQVGVPA
                     SFTNGQTFGTSVTFTGLVPGGSYTARVARDCYPDGWSAAEEISWTMSLREGQVAQEVL
                     VYPNPSNGNFRIQLNGYATDNLVIVITNAIGQVVYNNSMSVNESITVKEISLNDLAAG
                     TYTLKLIDGNNVTTRSIVIE"
     gene            complement(3434..4102)
                     /gene="cmk"
                     /locus_tag="PK742_08555"
     CDS             complement(3434..4102)
                     /gene="cmk"
                     /locus_tag="PK742_08555"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQU76679.1"
                     /translation="MSVIIAIDGYAGTGKSTLARDLANALGYTYLDTGAMYRAVTLYL
                     LRNDISWLDAEKLGRALQNIRIEFRISPDNGKRHTYLNGEDVEADIRSMAVSGVVSEV
                     AAVSAVRRFLVEQQRQIGMEQDCVLDGRDIGTVVFPNARFKFFITAPMEVRIARRLGE
                     LFRAGADIDADAVQANLEKRDREETQREDSPLRPAPDAVHLDTGGLDREQQLAAALKH
                     IEAQ"
     gene            complement(4126..5187)
                     /gene="porQ"
                     /locus_tag="PK742_08560"
     CDS             complement(4126..5187)
                     /gene="porQ"
                     /locus_tag="PK742_08560"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HQU76680.1"
                     /translation="MKLLSCRSLTTMQRLLLFFLFMPGLAKAQLGGSYTYQYLQLPVS
                     ARSAALGTWNVSTADEDLSMAWYNPAVLNDSMHNRLHINHALRAGNVNSGLVAYGRKL
                     DDRPVNTWAGLMYNTYGQMPAFDETGQQTGTFSASEFALVSGISYTAGNLRYGTNIRL
                     LFGQLENYHSLGLAMDIGAHYHLDEKRLDAGLVLRNIGAQLTSYTPSGREELPFEIQA
                     GISKKLAYLPLRITLTGHNLERFDIRYDDPSNASATIFQLDSNQAEKTYFGDKLLRHL
                     VLSGEFYFGRNFRARVGYDYMQQKEMVLTTYRGLTGFSLGFGFQINKYAVDYGYEIAS
                     VSGGNHFLTISADLDEFLR"
     gene            5303..5710
                     /locus_tag="PK742_08565"
     CDS             5303..5710
                     /locus_tag="PK742_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76681.1"
                     /translation="MKKIFLNGLITGAILLLLSYLLLVITVRVLPDLAWEYFGPSFNT
                     DSSRNVLFYIHPFLLGLALAWFWDRFKGLFKGVWILKGLEMGIMYALIATLPSMWITF
                     SAINVSFEMIGTWFLYGLFQSIIAGEVLARLNP"
     gene            complement(5707..>6302)
                     /locus_tag="PK742_08570"
     CDS             complement(5707..>6302)
                     /locus_tag="PK742_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQU76682.1"
                     /translation="ILGAPRFMKEDAAQLDSPGVAIGLAWTSVGGDILYIETSKSAGK
                     GGLQLTGNLGNVMKESASTALSYIRANAKTIGIPEDVFEKSSLHIHVPEGAIPKDGPS
                     AGITMLTSLTSALTGRKVKSGYAMTGEITLRGKVLPVGGIKEKILAARRAGLKHIILC
                     KANEKDIAEIPAAYLKGMRFHYVSDMSEVLGLALMKR"
BASE COUNT         1676 a         1580 c         1497 g         1549 t
ORIGIN      
        1 gacctgcgaa aatcgggtga ccaggatcag ctgcttcgcc ggtaccggtg aaataagagt
       61 agatcaggga agtaccgtca aagccgaaac tcatgccatc accttcgtta ggagcagcat
      121 tcagaagtaa acggcctcct ccggaaaccc atgattcaat ggttgtcatg ttggcatcca
      181 ggaaagtttc cagttcattg gcatgagcat ctgatccttc caggaatacg aaacagttct
      241 ctgaactgaa gagcacaaaa ggatcggcag tttcatagtg ttccatgctc catccgcctg
      301 caccgaaaac ggcatccatg gcatcggtgt tcgaagtaga accccagggt tcaccggttg
      361 tattggaatt cacataccag gcgccggtgc cggttacagg accagcgtta cacacggtct
      421 tgctgtagga gtcgtctccg tcaaaggaat cgtcagccca gtctatccag gccaggaaat
      481 catagcaacc ataatctgag aaagtaaaat catcgccaaa ggtgaagtcc acagattcgc
      541 cggctgccag tgttccgcca tacacttcag aagtgatcag gccaccatct acattatagc
      601 tgatggtgaa tccggatgca tcgttagcac cactgttcca gatagaaaca gtgacattca
      661 cagttccgag gtcagcacct gaagcaggag caggaatacc ggttacggtc aggttgtttt
      721 ccagcgggcc tgacaggttc actgtgtaat cttcagcttc accataggtc tggctgctgc
      781 agggatccag cggagtacca aggcctgaag gatatacacc tctcacacgc atacgggttt
      841 cacctgccag tgcagttaca ggcactgtaa aggtgatatc accggtagct gcagcgctca
      901 gactcaggtt accaagtacc tcatctgtgt caaacacttc atcctggttg aaatcgatcc
      961 aggcagtgat ggttgttgac caggatgggc catttgtaac tgacagggtg taggtagcac
     1021 cgttagaaag gtcggtagaa agatctgtat aatcagaata gccgatgtct gtgatctcac
     1081 ccccgctggc agtgttggaa atgtctccca gtactacccc atcaatacgg tcattgtcgc
     1141 tcgtaccggt ggtgtacact ggtgtacaat actgggcaaa tgcggtagct ccgcccagca
     1201 gcattgctac agctaatgct gaaagcttgt gtcttgtttt catcataatt gagattttga
     1261 ttatcctgtt aaggtataaa aaaagggggc aaatttgccc ccttttttta tgattttcat
     1321 caaaaattac tcgatcacaa tgctacgggt agtaacgttg ttgccatcga tcagtttcag
     1381 ggtataagta cctgcagcca gatcgttcag gctgatctct ttcacagtga tgctttcgtt
     1441 cacagacatg ctgttgttgt aaaccacctg gccaatggca ttggtgatca cgataaccag
     1501 gttatcagtt gcataaccat tgagctgaat gcggaagtta ccgttgctgg ggttcggata
     1561 aaccagaact tcctgagcaa cctgaccttc gcgaaggctc atggtccagc tgatctcttc
     1621 agcagcagac catccgtcag gatagcagtc gcgggctaca cgggcagtat agctgccacc
     1681 tggtaccaga ccggtaaagg ttacggaagt accaaaggtc tgaccattag tgaaggaagc
     1741 aggaacacca acctgaccgt aagcaaaacg atacttggta gcacccatgt catcccagct
     1801 cagggtagca gaagtagaac ctgcatctgc agaaagaccg gtaacagagt tacaagcttc
     1861 accgtcaacg aggttcacag tataatcttc tgtttcaccg aatgaagagc tgatctcgca
     1921 ggcagccatc tgatccatgc tggaagtaca gtcatctgca tagtcagcca gtacacgcat
     1981 acgggtagtt ccgggagctg ttccaagcgg aaccatgatg gtataggtag ctccgccggt
     2041 gccggcatct accagtgtac aacccagtct ttcgtcagca gagaaagtac catctacgtt
     2101 catgtcaata taaacggcaa taccctgatc ccatgaagga ccacagtcaa cgaatacttc
     2161 atactcagca ccgatggtca gatcagttga caggtaggtg aaatcgttat agttatcacc
     2221 agactcgccg gtatcaaaat tgctgatatc agcaacagag aaatcatcta cataatcacc
     2281 agagaggaag gatgtataat caggaatgca ataacagtca gtagctgcat tctgtccaac
     2341 ccaaacgctg gcagaagtac cgctttcacc gctgcctgca caggttacca cacactggta
     2401 ataggtagct tcagactggg tagcatcata gcttgaagcg gtagcaccgg caatatcgct
     2461 gtagctgatg ccatcagcag aactttgcca ctgatagctg attccgaagc cggtaattgc
     2521 atcaacagac agtgtaaaca catctgcagc acaaactgat tcagcagaag aaacagcagc
     2581 acttacatca ggagtacctg tacactcatc acctacggca ccatagaata tggtaaccgg
     2641 acgacgggta gggttggtac cggtagtagc tgctgctgaa taggtacaag ggctgagttc
     2701 ggtatcagac ctgaaggtcc tggaagagtt gtatgcagtt gtagcataag caaccacgcc
     2761 gttgtcggta taagtaccgg cactgcttcc accacagatc tcaatcacga tattgctggt
     2821 accatcccag gtaaaaggaa catccagcac gaaattgttc atagcagaag tagttggtgt
     2881 gaaatccatc ggaccccaaa cgatggtggc gccttcttcc caggcagcac ccaggtctgt
     2941 agtagcggtc atcatcattt taatagtgta tccttcggtc agaccgggaa tgtcagtagc
     3001 aatgaccggt gcattacagt accaggccag ttcggtgata tcaccggcac ccatacccat
     3061 agcagaaagt tctgacgcag ggtacagata ctgaacacgc tgggtcttgt accagtcgcc
     3121 gaaaggagta ggataagtag tattggtatt cacaccaaca ctatctccta tggagaaagc
     3181 ggtttgtgca gacaagctgc cggtaccgat aaacagcgca agggctgcaa caccggacca
     3241 tcgaagtaat gatcttttca ttgaatcatt tttattggtt aataatcagt tccccgtggc
     3301 ggagatcatc cggttcggga aggtaaattt tgcaagaaat tctctttttt acaaatgcct
     3361 gacagataaa attaacttgc cgacattaca gaaaaggctg tcatgtcaca aaccaaagtt
     3421 gctttggcaa caattactgt gcttctatat gcttcagagc agcagccaac tgttgttctc
     3481 tgtccagacc accggtgtcc agatgaacag catccggagc aggcctgagc ggactgtctt
     3541 cccgctgtgt ctcttcacgg tctctttttt ccagattagc ctgcacggca tctgcgtcaa
     3601 tatcggctcc ggcacggaac agttcaccca gtcttcgggc tatacgcact tccataggtg
     3661 ctgtaatgaa gaacttaaaa cgggcattgg gaaaaacaac ggttcctata tctctgccat
     3721 ccagcacaca atcctgctcc atgccgatct gtctttgctg ttctaccaga aatctcctga
     3781 ctgctgaaac agccgccact tcacttacca cacccgaaac tgccatgctg cgaatgtctg
     3841 cttccacatc ttcaccattc agataggtat gacgcttacc attgtcagga cttatcctga
     3901 actcaatacg gatattttgc agagccctgc ccagtttttc cgcgtccagc caggagatgt
     3961 cattgcgcag caggtacaga gtaacagcac ggtacattgc gccggtatcc agataagtat
     4021 agcccagtgc attggccaga tcgcgggcaa gggtgctttt gccggttcct gcgtaaccat
     4081 caatggcaat gatgacagac atgttgcgaa gataagcgac tgtctttatc gcagaaattc
     4141 atccagatct gcagatatgg tcaggaaatg gtttccgcct gacacactgg cgatctcata
     4201 accatagtcc actgcgtatt tgttgatctg aaatccgaaa ccaagagaga agcctgtcag
     4261 accgcggtag gtggtcagaa ccatctcttt ttgctgcatg tagtcatagc ctaccctggc
     4321 acgaaaattc cggccaaaat aaaactcgcc tgacagtacc agatgcctca gtaatttatc
     4381 gccgaagtat gttttttcag cctggttgct gtccagctga aagatggtgg cagaagcatt
     4441 tgatgggtcg tcatagcgta tatcgaagcg ctccaggttg tgacctgtca atgtgatcct
     4501 gagtggaagg taggccagct ttttgctgat tccggcctgg atctcaaaag gcagttcttc
     4561 tctgccggag ggagtatagc tggtcaactg ggcaccgatg ttgcgcaata ccagtcctgc
     4621 atccaaccgc ttttcatcaa gatgatagtg tgccccgata tccattgcaa gtcccagaga
     4681 atgataattc tccagctgac cgaataacag cctgatattg gtgccgtacc gcaggttccc
     4741 ggcagtatag gagattcctg aaaccaatgc gaactctgat gcagaaaagg tgcctgtctg
     4801 ctgaccggtt tcatcaaatg ccggcatctg cccataggta ttgtacatga gtcctgccca
     4861 ggtatttaca ggcctgtcat caagttttct gccataagca accaggccgc tgttcacatt
     4921 tccggcacgg agtgcatggt tgatatgcag cctgttatgc atggaatcat tcagcaccgc
     4981 aggattatac caggccatag acaggtcttc atctgcagtt gacacattcc aggtacccaa
     5041 agctgcagaa cgggcactaa caggcaactg aaggtactgg taggtatagc ttcctcctaa
     5101 ctgagccttc gccaggccgg gcatgaacag aaaaaacaaa agaagacgtt gcatggtggt
     5161 gagtgaacgg caagacagca gcttcattgc cggcaaacaa aaataaggca tccggctgac
     5221 agaaaaagaa aggtcaaaaa ggagataaaa gtcaatcaat ccaagtcaac catattttac
     5281 cttgcaccca aaacccaaca acatgaaaaa gatctttctg aacggtttga taacaggagc
     5341 tattttgctg ttgctcagct acctcctgtt agtgatcact gtgcgtgtat tgcccgacct
     5401 ggcctgggaa tattttggcc cgagcttcaa caccgacagc agcaggaatg tgctgtttta
     5461 catccacccc tttctgttag gtctggcttt agcctggttc tgggacagat tcaaaggcct
     5521 tttcaaaggc gtctggatac tgaaaggcct ggaaatgggc atcatgtatg cactgattgc
     5581 caccctgcct tccatgtgga tcaccttcag cgccatcaat gtttcttttg aaatgatagg
     5641 cacctggttt ctgtacggat tgtttcaaag catcattgcc ggagaagttc ttgccaggct
     5701 gaacccctaa cgtttcatca gtgccagacc gagtacctca ctcatgtctg acacatagtg
     5761 aaagcgcata cctttcaggt aggctgcagg aatttcagct atgtctttct cattggcttt
     5821 gcataatatg atatgcttca gtccggctcg tctggcagca agtatcttct ccttgatacc
     5881 accaacagga agaacttttc ctcgaagggt gatctcgccg gtcatcgcat accctgattt
     5941 cacctttctg ccggtcagtg cagaagtaag ggaagtaagc atagtgatac ctgctgacgg
     6001 accgtctttg ggaatggccc cttcgggtac atgaatgtga agactggatt tttcaaagac
     6061 atcttccggt atgccgatgg ttttggcatt ggccctgata taactcaggg cagtggaagc
     6121 actttccttc atgacattgc ccaggttacc ggtcagctgc aacccgcctt ttcctgcgct
     6181 cttgctggtt tcaatataca ggatatctcc tccaactgaa gtccatgcca gcccgatggc
     6241 tacccccgga gagtccagtt gtgccgcatc ttctttcatg aacctgggag caccaagaat
     6301 at
//
LOCUS       DAOMAX010000153         6292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1988_length_6292_cov_3.959596, whole genome shotgun sequence.
ACCESSION   DAOMAX010000153 DAOMAX010000000
VERSION     DAOMAX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_6292_cov_3.959596"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK742_08575"
     rRNA            complement(<1..539)
                     /locus_tag="PK742_08575"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            947..2017
                     /gene="serC"
                     /locus_tag="PK742_08580"
     CDS             947..2017
                     /gene="serC"
                     /locus_tag="PK742_08580"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQU76683.1"
                     /translation="MRVHNFNAGPAILPEAALAQASEAVKEFNGLGMSILEISHRSKD
                     FQAVMDEAGQLVLENLKLDPSYKVLFLQGGASTQFAMVPMNLLNSGDVAGYVNTGTWA
                     KKAIKEAKLFGKTEVLASSDGSNFSYIPKGFAIPENLRYLHLTSNNTIYGTQYMEYPD
                     SPVPVVIDMSSDIFSRNVNYGQFDLIYAGAQKNLGPAGVTLVIIKDGLLRESDHALPS
                     MLNYQTHIEGGSMYNTPPVFSIYVCLLTLRWLRDNGGAAAMEVMNQRKAAKLYAEIDR
                     NSMFKGTVAVEDRSIMNVNFIMENRDLEPAFLQFTKEHQCVGLNGHRSVGGFRASIYN
                     ALPESSVDHLVGLMQEFESKNA"
     gene            2028..2948
                     /locus_tag="PK742_08585"
     CDS             2028..2948
                     /locus_tag="PK742_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQU76684.1"
                     /translation="MSVILLATEKPFAADAVNKIRNVVAESTHTLQLLESYTDKQQLL
                     DAVAQADALIVRSDKVDADVIAAGKKLQIIVRAGAGYDNIDVAAATEKNVCVMNTPGQ
                     NANAVAELVFGFMIYQSRKQFSGSSGTELRGKKLGVVGFGNIGKLVAQIGKAMGMEVC
                     AYDKFIEAHVMQEKGIKPLATIEDVFEECHYISLHIPAVSDTKGSVDFHLLKTMRKNA
                     MLINTARQEVVNEDDIARIMEERSDISFVSDFMPKSDKLLDESMRKRWFAPVKKMGAQ
                     TEEANVNAGIAAAKQIIRFFDSGDRTFQLN"
     gene            complement(2949..3407)
                     /locus_tag="PK742_08590"
     CDS             complement(2949..3407)
                     /locus_tag="PK742_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76685.1"
                     /translation="MQVLLFLAASLAPVLTDDLIHTTREFYFDMQDGCPTEGFCLEDF
                     SMILTFDVGVTMQDEIREADFKDADLSFGVKQKFDQTTQHLKFTKYEKSFDRSTRKLI
                     LTLYPDELPNNRKSFVLKCVFEGQVKERGGTSGTLIFYLRNGSTYTYTYL"
     gene            3564..4808
                     /locus_tag="PK742_08595"
     CDS             3564..4808
                     /locus_tag="PK742_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HQU76686.1"
                     /translation="MSVIKPFKGLRPRPELAKEVASRPYDVLNSEEAREEAAGNQYSF
                     LHVCKPEIDLPEETDHYSQEVYDKGKENLAQMIREGILIQDETPCLYAYRQIMNGHGQ
                     VGLVANSGIDDYFNDIIKKHEFTRPEKENDRIRHMATLSCHPEPVFLTYPDVDEITPI
                     MEAVVARKAVYDFTSEDGIQHTFWVIDDASEINVITEIFREKVPFTYIADGHHRSASS
                     AKVGQQLREANPGHTGEEEFNFFLSVLFPSSQLMIMDYNRLVKDLNGHTPMDFFRMIA
                     ERFDIKHTGTEPYKPEVLHTFGLYYDGHWYQLTAKPGTYDDMDPIKCLDVTVLSDHLL
                     DPVLGIKDQRTDKRIDFVGGIRGLHELEKRVDSGEMKLAISLFPVSIDQLIHIADSGN
                     VMPPKSTWFEPKLRSGLIVHAF"
     gene            complement(4812..>6292)
                     /locus_tag="PK742_08600"
     CDS             complement(4812..>6292)
                     /locus_tag="PK742_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76687.1"
                     /translation="QFFLVDAVGCTFQDSFYVNYDTCYLHYAVLPEDTLRLCPGDSLL
                     LVNQYDAVSGYLWSTEDTTKQIWVYESGWYGLELTWSGCGSSIDSVFVDDAEPILGSV
                     LSIPASDSLHPDGSLIFIPDPLLSDYSYLWSNGDADSLAEELSPGYYEVLVTDSYGCN
                     FSFSGAVDTGICFHYYFDLGPDTSGCAGNAIVLSPVGTASGNYLWSDSSTNSVLSVLV
                     PDTVFLTISDSICSYSDTVLVAADVLPGLLIDVIPASRPINADGAIELTFLEDDPENF
                     MIAWSTGSSGTAISGLDTGYYSFTLTDTSGCSFFESLYLDFDSCYLPYGIYVSDTTNT
                     SFRVSWTGPEIASYVAFYRNLTTGGGFTSIPVPEGETSVDIPALLGQQIKYKIKFACA
                     GTVSESPLITFNMPEDSVLRVAENSTIHVWPNPFENDLYVTTDKEISWIITDLFGQIH
                     RQGWITAEGSFTTIDTRWLPSGVYILILKSDQSMHTVQLIHK"
BASE COUNT         1742 a         1463 c         1457 g         1630 t
ORIGIN      
        1 gcaccctccg tattaccgcg gctgctggca cggagttagc cggtgcttat tcgtagggta
       61 ccgtcagctc cggataaatc cggaggtttc ttcccctata aaagaagttt acaatccata
      121 ggacattcat ccttcacgcg gcatggctgg gtcagacttc cgtccattgc ccaatattcc
      181 ctactgctgc ctcccgtagg agtcgggccc gtgtctcagt gcccgtgtgg ctgatcatgc
      241 tctcacatca gctactgatc gatggcttgg tgagccgtta cctcaccaac tacctaatca
      301 gacgcacgcc catcttcatc cgccgtagct ttaaccaaga cctcatgcga ggtccgggtt
      361 ctatggggta ttatttccag tttcccggag ctatccccct gatgaaggta ggttgcatac
      421 gtgttactca cccgtttgcc ggtttgggtc aagtgcaagc actcgacttt tccctcgact
      481 tgcatgtatt aagcctgccg ctagcgttca tcctgagcca gtatcaaact ctccattgtt
      541 aaaagaattc gtaattgatt ggctccttac ctttttattt tttggttgct tctgtccaat
      601 acttcaaaga acttatgaaa aacccgttcg agaacaggcc acttcatcaa aatgggtcac
      661 aaagttaccg ctgctttatc cgctcttcca aattttcaga catctttttt ttcaacagtg
      721 tttacaccct gctgaaaagc ttatgtccgc atggctatcg gaaggcctgt cagcctgccc
      781 ttcacttcta ttcaacgcct ggctcctgca cctgcagaaa gtcatttttt gacctccctt
      841 taagttgtac ttttgagccg ccttagctgc gatcagtaac cttttaagaa cgaatgggag
      901 cgcaaaggta tgaaagaatt taaaacctcc aaataaaaag taaaaaatgc gagtacataa
      961 tttcaatgcc ggacctgcca ttcttcctga agccgctctg gcacaggctt ctgaggccgt
     1021 aaaagaattt aacggactgg gtatgtctat cctggaaatc tctcaccgaa gcaaagattt
     1081 tcaggctgta atggatgaag cagggcaact cgttctggaa aatcttaagc tggatccctc
     1141 ctacaaggtt ctgttcctgc agggcggagc ttctacccag ttcgcaatgg tgcccatgaa
     1201 tctcctgaac agtggcgacg ttgccggcta tgtaaacacc ggtacctggg ccaagaaagc
     1261 catcaaggaa gctaagcttt ttggcaaaac agaagtgctg gcaagcagtg acgggtctaa
     1321 tttcagttat atacccaaag gttttgccat acctgaaaac ctgcgatatc tgcacctgac
     1381 ctctaacaat accatatatg gtacccagta tatggagtat ccggattcgc ctgtaccggt
     1441 tgtgatcgat atgtcatcag acattttcag ccgaaatgtc aattatggtc agtttgacct
     1501 gatctatgca ggtgcacaga agaacctggg gccagcaggt gtcacgctgg ttatcattaa
     1561 ggacggtttg ttgcgggaat cagaccatgc ccttccttct atgctgaatt atcagacaca
     1621 tattgagggt ggttccatgt acaatactcc acctgtgttc tccatatatg tgtgcctgct
     1681 gacccttcgc tggttgcggg ataatggtgg tgccgctgcc atggaggtca tgaatcagcg
     1741 caaagctgca aagctgtatg cagagataga ccggaacagt atgttcaaag gcacagttgc
     1801 ggtagaagac cgttctatca tgaatgtcaa tttcataatg gaaaaccggg atctggaacc
     1861 tgcatttctt cagttcacca aagaacacca gtgtgtggga ttgaatggcc accggtctgt
     1921 gggtggtttc agggcttcta tttataatgc attgccagag tccagcgtgg accaccttgt
     1981 tgggttgatg caggaattcg aatccaagaa cgcataaaat caataatatg tcagtaatac
     2041 ttctcgcaac cgaaaagcct tttgcagcag atgcggtcaa taagatcagg aatgtggtag
     2101 ccgaaagcac ccacacactg caattgctgg aatcttacac ggacaaacaa caactgcttg
     2161 atgcggttgc tcaggctgat gcactgatcg tacggagcga taaagtggat gccgatgtca
     2221 tagctgccgg taaaaaacta cagatcattg ttcgagccgg agccggatac gataacattg
     2281 atgtagcggc tgctactgaa aaaaatgttt gcgtcatgaa tacaccgggg caaaatgcca
     2341 atgcggtagc tgagctggtt ttcggattca tgatctacca aagtcgcaag caattttcag
     2401 gaagctcagg aactgagctt cgcgggaaaa aactgggtgt ggtaggtttt ggcaatatcg
     2461 gcaagctcgt ggctcaaatc ggcaaggcta tgggcatgga agtttgtgcg tatgacaaat
     2521 tcattgaagc acatgtcatg caggagaagg gaatcaaacc attggctacc atcgaagacg
     2581 tctttgaaga atgccattat atcagtctgc acattcccgc ggtctctgat accaagggct
     2641 cagtagattt ccatttactg aagaccatgc ggaagaatgc catgctgatc aacacggcac
     2701 gtcaggaagt ggtcaatgaa gatgatattg ccaggatcat ggaggaacgt tctgacatca
     2761 gctttgtgag tgatttcatg cctaagtcag acaaactgct tgacgagtca atgcgaaagc
     2821 gctggtttgc tccggtcaaa aaaatgggcg cccaaacgga agaagcaaat gtcaatgccg
     2881 gtatcgcggc agcaaagcag atcattcgtt tctttgattc aggtgaccga acatttcagc
     2941 tgaactgatc aaagatatgt gtaggtatag gtggatccgt tccggagata gaatatcaat
     3001 gtaccggagg ttccacctct ttctttcacc tgtccttcaa atacgcattt cagtacgaat
     3061 gacttccgat tgttcggcag ttcatccgga tacaaggtaa gaatcagttt tcttgttgac
     3121 cggtcaaatg acttttcata tttggtgaac ttcaggtgct gcgtagtctg atcgaatttt
     3181 tgttttaccc caaaagaaag atctgcgtct ttaaagtctg cctcccggat ctcatcctgc
     3241 atagtaactc caacatcaaa ggtcaggatc atgctgaaat cttccagaca aaaaccttca
     3301 gttgggcatc cgtcctgcat gtcaaaatag aactctcttg tggtgtgtat caggtcatca
     3361 gtgagaacag gtgccagact ggcagccaga aacaaaagta cttgcatgat ctccaatatt
     3421 agtggtgtaa agctattgac atgtttgcgg agcagttaat tatgtcaaga acgtttgtac
     3481 acctgatttt cgtatctgac cccgatagtt gacaatgatt ccgctatctc agaattgtat
     3541 ctttgcagca aaatttccag aagatgtcgg tcatcaaacc attcaagggg ctacgtcccc
     3601 gtcctgaact ggctaaagag gttgccagca gaccctatga tgtacttaac agtgaagaag
     3661 cccgagaaga ggctgcggga aatcaatatt cttttttgca tgtgtgcaaa cctgagatcg
     3721 accttcctga agaaactgac cactatagcc aggaggtata tgataagggc aaggagaacc
     3781 ttgcacaaat gatccgggag ggcattctta tccaggacga aactccctgt ttgtatgcat
     3841 accgtcagat catgaacggg catggtcagg tggggcttgt ggccaattca ggcattgatg
     3901 attatttcaa cgacatcatc aaaaagcacg agttcacccg tcctgaaaaa gagaatgacc
     3961 gaatcaggca tatggctaca ttgtcatgcc atccggaacc ggtatttctg acctatccgg
     4021 atgtagatga gatcaccccg atcatggaag cggttgtggc acgaaaagct gtatatgatt
     4081 tcacatcgga agatggcatc caacacactt tctgggtcat tgatgatgca tctgagatca
     4141 atgtgatcac agagatcttt cgtgagaaag ttccgttcac ctatattgcc gacggtcatc
     4201 accgaagtgc cagcagtgcc aaagtaggtc agcaactccg ggaggctaat ccgggtcata
     4261 ccggagaaga ggagttcaat ttcttcctgt ctgtactctt cccatccagc caactgatga
     4321 tcatggacta taacaggctg gtaaaagacc tgaatggcca tactccaatg gactttttca
     4381 ggatgattgc agaacgtttt gacatcaagc atactggcac tgaaccctac aagcctgaag
     4441 tactccatac ttttgggctt tactatgacg ggcattggta tcaactcact gcaaagccgg
     4501 gaacctatga tgatatggat ccgataaaat gtctggatgt aactgttctg tcagatcatt
     4561 tacttgaccc cgtattgggc atcaaagacc agcgaacaga caaacgaata gattttgttg
     4621 gtggcatcag aggcctgcat gaattggaaa aacgggtgga cagcggagaa atgaagctgg
     4681 ccatttctct gtttccggtt tccattgatc agttgatcca tattgcagat tccggaaatg
     4741 tgatgccacc taaatccacc tggttcgaac ccaagctacg cagcggactt attgtgcatg
     4801 cattctgatg tctatttgtg aataagctgc accgtatgca tgctttgatc acttttcagg
     4861 atcagaatat atactccgga aggtaaccat cgggtgtcta tagttgtaaa ggagccttct
     4921 gctgtgatcc atccctgcct atgtatctga ccaaataggt ctgtaatgat ccaggatatt
     4981 tctttatcag tagtgacata caggtcattt tcaaatggat ttggccagac atgaattgta
     5041 ctattttcag ccacacgaag tacagaatct tcaggcatgt tgaatgtaat gagcggcgat
     5101 tctgaaacag tgcctgcaca tgcaaatttg atcttgtatt tgatctgttg cccaagcaga
     5161 gcaggtatat caacagatgt ttcgccttcc ggcaccggaa tgctggtaaa gcctccgcct
     5221 gttgtcagat ttcggtagaa tgccacatag ctggctattt caggacctgt ccaggacacc
     5281 ctgaaagaag tattggtggt gtcactgaca tagatcccat aagggaggta gcaactatca
     5341 aagtccagat acaggctttc aaaaaaagaa caccctgaag tgtctgtaag agtaaaggaa
     5401 taatatccag tatccagacc gctgatggca gtacctgatg atcctgtgga ccatgcaatc
     5461 ataaagtttt ccggatcatc ttccagaaat gtgagttcaa tggcaccgtc agcattgata
     5521 ggccgggagg ccggtatcac atcaatcagc agtccaggca gcacgtctgc tgctaccagc
     5581 acagtatctg aataagaaca gatactgtct gatatagtca ggaaaacagt atccggaacc
     5641 agcacagaca gaacactgtt cgtgctgctg tcactccaca gataattgcc tgacgccgtc
     5701 cctacaggtg ataacacaat tgcattccct gcgcaaccgg aagtatccgg ccccaggtca
     5761 aagtagtaat ggaagcaaat cccggtatcc actgcgccgg aaaatgagaa attgcaccca
     5821 taactgtcag tgaccaatac ctcgtaataa ccaggcgaca gctcttctgc cagtgaatca
     5881 gcatctccgt tgctccacaa atatgaataa tctgagagaa gcggatcagg aataaagatc
     5941 agtgatccgt ctggatgtaa actatcggaa gcaggtattg atagcactga acccagtatc
     6001 ggttcagcat catccacaaa tacagaatca atggaagagc cacatccgga ccaggtcagt
     6061 tccaggccat accaacctga ttcataaacc cagatctgct tggtggtatc ttccgttgac
     6121 cagaggtatc cggaaactgc atcatactga ttaaccagca gaagactatc gcccggacaa
     6181 aggcggagcg tatcttccgg taacacagca tagtgcaaat aacatgtatc atagtttaca
     6241 tagaaggagt cctgaaaagt gcatccaact gcatctacca gaaaaaactg gt
//
LOCUS       DAOMAX010000154         6266 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_1998_length_6266_cov_4.017389, whole genome shotgun sequence.
ACCESSION   DAOMAX010000154 DAOMAX010000000
VERSION     DAOMAX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_6266_cov_4.017389"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PK742_08605"
     CDS             <1..343
                     /locus_tag="PK742_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76688.1"
                     /translation="LDGPQWNSCDPDGRLANGQMLSYGDDNYVTAEEVMLYPSPNDGT
                     FSIKLAKSHTGYAEVTVVNNMGQQVYRSTYDSFIANKNINIRLEDATPGIYFLNINLD
                     GNITTKEFVVK"
     gene            complement(484..1518)
                     /gene="thiL"
                     /locus_tag="PK742_08610"
     CDS             complement(484..1518)
                     /gene="thiL"
                     /locus_tag="PK742_08610"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQU76689.1"
                     /translation="MTDNDRTEISDLGEFGLIEHLTANFELKQAFSLKGIGDDAAVVD
                     HQGRLTVVTTDLLLEHVHFDLMYMPMKHLGYKSVVVNLSDLYAMNAEPKQITVSVALS
                     NRFSVEAMEELYDGIHIACERYGVDLIGGDTSSSVKGLVISVTALGEANEDELVYRDG
                     AKPGDILCVSGDLGAAYMGLQLLEREKRIYIENPQIQPDLEGHPYIVGRQLKPEARRD
                     VIELLREKGLKPTSMIDLSDGLSSDLQHICRQSGTGALVYESKLPIHEETYNMALEFH
                     MDPTTAMLNGGEDYELLFTLPADESYKVLEMEGITIIGKITDASEGIRLETKNGNEQE
                     MPAQGWNHLG"
     gene            1569..3236
                     /locus_tag="PK742_08615"
     CDS             1569..3236
                     /locus_tag="PK742_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQU76690.1"
                     /translation="MSTGVEVRSPLEMLYNWEQEKPEVLYLRQPIEGQWINYNWARVA
                     DEVRRMAAWLQAKGYEPGSKVAILSKNCVHWVLADWAIWMAGYVSVPIYPNVTPHTLN
                     QILEHSESKLCFVGKLDDWPYLKPGVPAAMDRVSFPFYGINEFPNWDEVLQGVEPMKE
                     NVVYPMDNICTIMYTSGTTGIPKGVMHSFFNFAYAASNALPPLHIGQEATFFSYLPMC
                     HIAERLLIEMGSLYAGGQVSFAESLDTFPKNLADTQPTVFLAVPRIWAKFQEKILEKM
                     PQKKLSLLLSIPVINGIVKNKIRKTLGLAKATNIFSGAAPISVELINWFGKLGIQIQQ
                     AYAMTEDSCYSHVNIKWRDKVGTVGEALPDVEVRISEEGEIQIRHEALMKGYYKEPGM
                     TAEVFTPDGYFKTGDKGERDSEGYLRITGRVKDLFKTSKGKYVAPGPIEMKLMDDPDI
                     EQACVVGLGLPQPIALVTLSESGRKKTERDLEHVLRTTMFHINEGLDSFEKMQKIIII
                     TDEWTIDNGLMTPTLKIKRGDVEKKYQMNYEKWYDIKGDILWEEVES"
     gene            complement(3446..>6266)
                     /locus_tag="PK742_08620"
     CDS             complement(3446..>6266)
                     /locus_tag="PK742_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQU76691.1"
                     /translation="QRSLGAGEIWMYHMSGGQGIQLTKRKNDQQDVGEPWASPDGRYV
                     YYSEDVYPGGYFQYNKDPNSQIYVINRYDLQEGKTEQVTGGPGGAVRPVVSHSGEWLA
                     FVKRVHDRSVLYIRELSTGREWPIYDQLDKDQQEAWAIFGPYTGFNFTPDDQYIIIWA
                     GGGIQRINVQTMEVSSIPFHATAVHPIVNALHFQQDPAPDNFRVKAIRQATTSPDGKT
                     LVFNAAGYLWKKSLPDGKPVRLTTGKDLEYEPAFSADGHYLAYVTWNDVEMGAIWQLD
                     LRNTKAVPQKLTTTKAIYREPAYSPKDPKVLVFRKEEGNTNQGYTNTLEPGIYLMHTD
                     REEAPEKITEEGMFPMFNADASRIYYQNGGYLFGDLTKTLVSVNLRGEDKREIVTSKY
                     AQRIVPGPDDQWVAFTNLYKVYVAALPMSGQTLDLDGQSKSIPVARVAHDAGINLQWS
                     ADASELRYTLGDAYYTVPLAERFSFLAASPDSLPPMDSVGISIGLDLPSDKPEGKVVF
                     THARIITMEGDEVIEDGTLVVQGNRILSVGTAYHPLVEEKNTTVIDCTGKTIMPGLID
                     VHAHLGQFRFGLSPQQHWQYFANLAYGVTTTHDPSSNTEMVFSQAEMVRSGVMVGPRI
                     FSTGVILYGADGDFKALINNLDDARFAINRTKAFGAFSVKSYNQPRREQRQQVIKAAS
                     ELGIQVVPEGGSTFFHNLTMILDGHTGVEHNLPVATLYDDVVQLWAASNTGYTPTLIV
                     NYGGLNGEYYWYQHTDVWKDSKLLTFTPRDVIDPRARHRTMVPEEEYENGHILVSQSC
                     KKLTDAGVRVNLGAHGQLQGLGAHWELWMLAQGGMTNMEALRSATVNGAYYLGMEDDL
                     GSLKPGKLADLIVLDKDPLEDIMNSNSVHYTMVNGRLYDASTMNETGNYDRKRLPFYW
                     ETGGYAPSFDWHGVTHTGCSCEAGN"
BASE COUNT         1517 a         1550 c         1567 g         1632 t
ORIGIN      
        1 tcttgatggt ccgcagtgga acagctgtga tcctgacgga cgtctggcca acgggcagat
       61 gctgtcttat ggagatgata attatgtgac tgccgaagag gtgatgttgt atccctctcc
      121 gaacgatggc acattcagta tcaaacttgc gaagtctcat accggttatg ctgaagttac
      181 agtggtgaat aatatgggac agcaggtata tcgcagtact tacgacagct tcattgccaa
      241 taagaatatc aacatccggc tggaagatgc cacccccggt atctatttcc tgaacatcaa
      301 cctggatggc aacatcacca ccaaggagtt tgtggtgaag tgaaggttga agggcaaaaa
      361 aggtagaagg gttacaagga ggaataggct atgacctttc atccttgtaa ccctttcgct
      421 ttgatatttt tcacaattat caaaccttcc accagaacca tatccgggtt caccctttat
      481 actttacccc aggtgattcc acccctgggc cggcatttcc tgttcgtttc cgttttttgt
      541 ttccagtctg atcccttctg aagcatcggt gatctttccg atgatggtga tgccttccat
      601 ctccagcacc ttgtatgatt catcggccgg cagggtgaac agcaattcat aatcttcccc
      661 gccattcagc atggcagttg taggatccat atgaaattcc agtgccatgt tataggtctc
      721 ttcgtgaatg ggaagtttgg actcatagac cagtgcaccg gttcctgatt gcctgcagat
      781 atgctggagg tcagatgata atccgtcgct cagatctatc atggaagtgg gcttcaaccc
      841 tttctcccgc aaaagttcaa tgacatctct tcttgcttcc ggtttcagct gtcggcccac
      901 gatgtacggg tgcccttcca ggtcaggttg gatctgtggg ttctctatat agatgcgctt
      961 ctctctttcc agcagttgca atcccatata ggcagcaccc agatcgccgc tcacacagag
     1021 aatatcgccg ggtttggctc catcgcggta caccagttca tcttcgttgg cttcacccag
     1081 tgctgttacg ctgatcacca gtcctttgac tgaagaggaa gtatcgccac caataaggtc
     1141 cactccgtaa cgttcgcagg caatatggat cccatcatac aattcttcca tcgcttccac
     1201 agagaacctg ttggacaggg ccacagacac tgtgatctgt ttcggttctg cgttcatggc
     1261 atagagatcg ctcaggttga ccacaacact tttatatccc aggtgtttca ttggcatgta
     1321 catcaggtca aaatgtacgt gttccagcag cagatcggtg gtcacaacgg tcaaacgccc
     1381 ctggtggtca accacagcgg catcatcgcc aattcctttg aggctgaatg cttgtttgag
     1441 ttcaaaattg gcggtcaggt gttcgatcaa tccgaattct cccaggtcac tgatctctgt
     1501 gcggtcgttg tctgtcatgc tgaaatgatt cttttgttat ttttaatcca gtcaaatgca
     1561 aagctactat gagtacagga gttgaggtaa gatcgccgct ggagatgctc tacaattggg
     1621 agcaggagaa gccggaagtg ctgtatctgc gacagcccat tgagggccag tggatcaatt
     1681 ataactgggc tcgggtagcc gatgaagtgc ggaggatggc tgcctggctg caggcaaaag
     1741 gctatgaacc cggcagtaaa gtggccattc tatccaagaa ctgtgtgcac tgggttctgg
     1801 cagactgggc catctggatg gcaggatatg tgtctgtacc catctatccg aatgtgaccc
     1861 ctcataccct gaaccagata ctggagcaca gtgaatcgaa gttgtgcttt gtggggaagc
     1921 tggatgactg gccctatctg aagccgggtg ttcctgcagc tatggatcgg gtttcattcc
     1981 cattctatgg tatcaatgaa tttcctaact gggatgaagt gctgcagggt gtggaaccta
     2041 tgaaagaaaa tgtagtgtat ccgatggata acatctgcac catcatgtac acgagcggta
     2101 ccaccggcat tccgaaaggg gtcatgcatt ctttctttaa tttcgcctat gccgcaagca
     2161 acgccttgcc accacttcac attggtcagg aagctacttt cttttcctat ctgcctatgt
     2221 gtcatattgc agaaaggttg cttatagaaa tgggaagttt gtatgccggc gggcaggtgt
     2281 ctttcgcaga atctcttgat acttttccaa agaatctggc tgatacccaa cccaccgttt
     2341 tcctggcagt acctcgtatc tgggccaagt tccaggaaaa gatcctggag aagatgccac
     2401 agaaaaagct cagtttgctg ctctccatac ctgttataaa cgggatcgtg aagaacaaga
     2461 tcaggaagac cctgggtctg gcaaaagcta ctaacatatt ctcaggcgca gcacccattt
     2521 cagttgagtt gatcaactgg ttcggtaaac tgggcattca gatccagcag gcctatgcca
     2581 tgaccgaaga ctcctgctat tctcatgtca atatcaaatg gcgtgataag gtgggcactg
     2641 taggtgaagc attgccggat gtagaagtgc gaatcagcga agaaggtgag atacagatca
     2701 gacacgaggc actcatgaaa ggatactaca aggaacccgg tatgaccgca gaggtattta
     2761 caccggatgg ctacttcaaa acgggtgata aaggagaacg agacagcgaa ggatatctgc
     2821 gcatcaccgg cagggtgaag gatctgttca agaccagcaa gggtaaatat gtggcccccg
     2881 gtccgataga aatgaaactc atggatgacc cggatattga acaggcctgt gtggtagggc
     2941 tcggattacc ccagcctatc gccctggtca ccctgagtga atcgggcagg aagaaaactg
     3001 aacgagacct ggaacatgta ttgcgtacta ctatgttcca tatcaatgaa ggactggact
     3061 cttttgagaa aatgcagaag atcatcatca ttaccgatga atggaccata gacaatggtc
     3121 tgatgacccc aaccctgaag atcaagcggg gtgacgtgga gaaaaagtac cagatgaact
     3181 acgaaaaatg gtatgatatc aaaggagaca ttctttggga agaagtggaa agctgatata
     3241 ccaacaactt cgggtgatcc attttgatgt ttcaggggga ggcctgcagc agtaagaaag
     3301 ccttacatac catccaacct aagccgaaaa ttgtttcggc ctggaaactt ttatgaagac
     3361 actgcaacgc atggagccat gatcatgact gaactgatga gcactgcctg ttagaaaggt
     3421 tcttttgcta ccagtttgtc ttgcctcagt tgcctgcctc acaggaacat ccggtgtggg
     3481 tcacaccatg ccagtcaaat gaaggtgcat agccacccgt ctcccagtag aacggcaacc
     3541 gtttccggtc atagtttcct gtttcgttca tggtacttgc atcatacaac ctgccgttta
     3601 ccatggtata gtgtacagaa ttgctgttca tgatgtcttc cagcggatct ttgtccagta
     3661 ctataaggtc tgccagcttg ccgggtttca atgaacccag atcatcttcc atacccaggt
     3721 aataggcgcc attcacagta gcagagcgca gggcttccat attggtcatc ccaccctgag
     3781 ccagcatcca cagttcccag tgcgcaccca gtccctgcag ttgtccgtgt gcaccaagat
     3841 tgacccgaac accggcatct gtaagcttct tgcaactctg tgataccagt atgtggccgt
     3901 tctcatattc ttcttccggt accatggtgc ggtgcctggc tctggggtcg atcacatcgc
     3961 ggggtgtgaa ggtcagcagt ttgctgtctt tccacacatc ggtatgctga taccagtagt
     4021 actcgccgtt aagtccgccg taattgacaa taagcgtcgg ggtataacct gtattgctgg
     4081 ccgcccagag ttgcaccaca tcgtcatata atgtagccac cggcaggttg tgttccacac
     4141 cggtatgtcc gtccaggatc atagtgaggt tatgaaagaa agtgctgcct ccttccggta
     4201 ccacctgaat gcccagttca gaagctgctt tgatgacctg ctgacgttgc tctcttctgg
     4261 gctggttgta actcttgacc gagaaggcac caaatgcctt tgttctgttg atggcaaaac
     4321 gggcgtcatc caggttgttg atcaacgctt tgaaatcgcc gtcggctccg tacaatatca
     4381 caccggtaga aaaaattctg ggacccacca tgacacctga tctcaccatt tccgcctgag
     4441 agaacaccat ctcggtattg ctggatgggt catgcgtggt ggtcacacca taggcgaggt
     4501 tggcaaagta ctgccagtgc tgctgcggac tcaatccgaa cctgaactgt ccgaggtgtg
     4561 catgcacatc tatcagtccg ggcatgatgg tcttgcctgt acagtcgatc actgtggtat
     4621 ttttttcttc caccagcgga tgataggcgg tacccacaga tagtatcctg ttgccctgca
     4681 ctaccagtgt gccatcttct atcacctcat cgccttccat cgtgatgatg cgggcatggg
     4741 tgaacactac tttcccttcc ggtttatcag atggcagatc aagaccgatg gagatcccga
     4801 cagaatccat gggtggaagt gaatcaggtg atgcagccag aaaactgaaa cgctctgcca
     4861 gcggaacagt gtagtaagca tcaccgaggg tatagcggag ttcagatgca tcggccgacc
     4921 attgcagatt gataccggca tcatgtgcca cccttgctac cggaatggat ttggactgcc
     4981 cgtccagatc aagtgtctga ccagacatag gaagtgctgc tacataaacc ttatacagat
     5041 tggtgaaggc tacccactgg tcatccggac cgggaacaat gcgttgcgca tatttcgatg
     5101 taacgatctc ccgcttgtct tcccctcgaa ggttgactga cacgagtgtt ttggtaagat
     5161 cgccgaacag gtaaccgccg ttctggtagt agatgcggga agcatctgcg ttgaacatcg
     5221 ggaacatacc ttcctctgtg atcttttcag gtgcctcctc acgatccgta tgcatcagat
     5281 agatgccggg ttcgagtgtg ttcgtatagc cctggttggt attgccttct tctttgcgga
     5341 agaccagcac cttcggatct ttaggtgaat aggctggttc gcggtagatg gctttggtgg
     5401 tggtcagttt ttgcggaacg gctttcgtat tgcgcagatc cagttgccag atggcaccca
     5461 tctccacatc attccatgtt acataagcca ggtaatgacc atcggcagaa aaagctggtt
     5521 catattcaag atcttttcct gttgtaagac ggaccggttt gccgtcgggt aatgattttt
     5581 tccagaggta acctgctgca ttgaacacca gggtcttccc atcgggggaa gtggtggcct
     5641 gacggatggc cttcacccgg aaattatcgg gtgcaggatc ctgttggaag tgcagggcat
     5701 tcacaatggg atgaaccgct gtggcgtgga aggggatgga gctgacctcc atggtctgca
     5761 cgttgatgcg ttgaatgccg ccgcctgccc agatgatgat gtactgatca tccggcgtga
     5821 agttgaatcc ggtataagga ccgaagatcg cccaggcttc ctgctggtct ttatccagct
     5881 gatcatagat gggccattct cttccggtgg acagttcgcg gatatacagt acagatctat
     5941 catgcaccct ttttacaaaa gccagccatt cgcctgaatg agaaaccaca ggcctgaccg
     6001 ctccgccggg tccgcctgtt acctgttctg ttttgccttc ctgcagatca tatctgttga
     6061 tcacatagat ctggctgttc ggatccttgt tgtactggaa gtaaccaccc ggatacacat
     6121 cttcactgta atagacatat cgtccatcag gagaagccca tggctcacct acatcctgct
     6181 ggtcattctt tcttttggta agctgaatac cctgtccgcc cgacatgtga tacatccata
     6241 tctcaccggc accaagcgat ctttga
//
LOCUS       DAOMAX010000155         6176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2029_length_6176_cov_3.697272, whole genome shotgun sequence.
ACCESSION   DAOMAX010000155 DAOMAX010000000
VERSION     DAOMAX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_6176_cov_3.697272"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6176)
                     /locus_tag="PK742_08625"
     CDS             complement(<1..>6176)
                     /locus_tag="PK742_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HQU76692.1"
                     /translation="MREFQNRDSEGDNTYKGVVYSSQSVDKMRAGFDMAKMDPIWLWK
                     NPKDGKLYVLSGHSRSTFFRMQADEGASGFDKIPAKIFEGISKEEALKLAIESNTLAS
                     KETDVERSKVYRKMREQGKSKTAILEAAKLAEGANAQKIIDISYLNPTGKIMTALNQF
                     GQSQSDQVENVVQIARWGGKLRAGYEQMTNAHEDELFDFLLKNPRYTNYDQVRAVVDK
                     AVNNLMFDPAQPMNLQRFISKSTYQQEFDADVAEKEKAIKDLEKNIEAKRKRYINEGL
                     DTQSITEMLSNDMALLNKLRTELVQFKQKGEANVKNAENSTIGLFDDLPIQKPEPKET
                     EAPAEEWDKNLFKARTYAADLLKADPAMQPYMDKMDWTDLDSIVAGINKYLAKEPVVE
                     PAKADPKKIGLSVRFADNYLGTAKGMKGVIESVNTDGTYNINLGKNAGGQVMRKKNVP
                     ADYLEETAFELSDLTKSRDAVIPDKKEAPEETPAEEAQPEPEPDTSKGDKLAELERLG
                     NEKLAAFRKMLKDKGKLGIISDPKKEAEDNAELIEALVELAEIFVQLGYYKFKAWAEK
                     IVSELGEVVREYLEEAWDSYTDQEDAEGNLLKVSEFAALKQKEEPELEVTPEETPVRP
                     QPKSQEDPATSGSTSAGVGGRIVGNGLTGVGTRKNARRGAMLIETGDIDLGTPEKRRA
                     ANLKAEQIIEAVESGEKVELTADDVRALMAYSGRGNISSYSTGTEYEHYTPLEVYQRV
                     YGVLSDMGFLDKVGSILIPGVGMGRELMAMPASLHDKMDSGDLETVIVDLPQSVGLKV
                     AKLLFPKTRAFAQYKDPVTRVSTPGLMGFKKAHGDKKFDLIITNVPFDANARMSDMAL
                     HDGFIAESLDLLAPGGYGVFIAPTGVADKVKGAQSIFATKADVVTAIRLPSNTFKDSA
                     STTVDADLLIFRKPTNTEAVISGFVNELGRDLPAVAVAQDVINTSDSALARKMVEGKF
                     NVTNYKKFQSLAVKLNMAEEELFPIVSTFAKLRQNREDFLKSEVRRVPLAYHEPNSQE
                     WFRKANVFLKGLVERKGDERLKRAFFEQGEGQIISMDAIVPLWDLIQEDIKSKITETV
                     TAADATNINPAYSLIAGTWQVEDILGPLSTAIQHLIHGLVNYDISNRILEGKSTKPHI
                     ITNLNQLIEGQHLTPHHTFRGPKVFGSEIDMGPESTRLQEMQEMRFMPDSELNKKVHI
                     KKVGDFMVKPADNVQPGTHIVIHGEQGDVLGRKSNVTPDRLTNMLYAGFEKEFGADSA
                     KKLRGVKGSISIEGLMANSDYRVTKKKQSRKEKLGESFEQMRRKQIMTMLKGVTDNLN
                     QQVKMANYIEAVLGSVTGSFYKEVAGTPELMDLLQTVDKILEYRDRANNNQLSDHEQS
                     IARKAAVGELQEFILRNGLESLSKSDLVKQLAATDEFKADARSYAITSIANLVEDEDG
                     KLMKPDFWEGLFGDKKVRYDEQQEFTSAVDLTNTIASREGLPKSFAVDHLIERIRASK
                     LRLNPELAERLNDEYEFTKALTATGEYMMDVTRRDALHVMATEDFAVGNIIEKIAFAT
                     EALDQMIGEGVDPINLDAAVAKLNELRASIPPIELKDVSLDPNAGVVHSLPDIIYGFA
                     DLMMGGDMRSAIVQKQIGSTVRWEFIGQSDNNLITEALSANGKSITRGMFARWMNRTD
                     EVKPMEGPQQGTFVGTDGKITSDPEKAKKIPDPETTTERNRVLTEKFNEFVKDHGLAD
                     QVKENYNNQFAYYEKKHSTGEMELRGLSKTFNGKPLKINPWQWKAVHHYMEYGRSFFN
                     HAVGAGKTMTAVLAAKAKMDKGLSKTAGFLVPLKTVQQWQDTWQQLFPDLRVVVVTSQ
                     NREQLFSDISFGGADVVIMPGSIIAPSRATLSPDKLVEYMHQDLEPFYDAQTNLTAAM
                     NHAELAGDKAAVKKYKKLVNDNQRKINSKLKVIESIMERSSGQVQTTLDSIGIDQLIV
                     DEADVFKNLETPLGDFSDIKGISTSSSGPAEIVRYWTRYTQDQTNYNNIAYLTATPVS
                     NSAVELYQSMNAIIPDSLESAGIRSLDDFLTEFADVISEEMAG"
BASE COUNT         1257 a         1718 c         1399 g         1802 t
ORIGIN      
        1 ccggccattt cctcggatat gacatcagca aattccgtca ggaaatcgtc aagagacctg
       61 atcccggcag actccaggct atcgggtatg atggcgttca tgctctgata cagctccaca
      121 gcggagttgc ttactggtgt agctgtcaga tacgcaatat tgttgtagtt ggtttgatcc
      181 tgagtgtatc tggtccagta ccggacgatc tctgcgggac cgctgctact ggtggaaatc
      241 cctttgatat cactgaaatc ccccagaggt gtctccaaat tcttgaacac atcggcttca
      301 tccactatca actgatctat tccaatggaa tccaatgtag tctgaacctg accgctggat
      361 cgctccatga tcgactcaat aaccttgagc tttgaattga tctttctctg attatcattt
      421 accagcttct tgtacttctt cacggcagcc ttgtcccccg caagttctgc atggttcatt
      481 gctgctgtca ggttcgtttg tgcgtcgtag aatggttcga gatcctgatg catgtactcc
      541 acgagcttat ccgggctgag tgttgctctg gatggcgcta taatactacc tggcatgatc
      601 accacgtccg cacccccgaa actgatatcg gaaaacagtt gctccctatt ctgagaagtc
      661 acaacaacaa cacgcagatc cgggaagagc tgctgccagg tgtcttgcca ctgctgtacg
      721 gtcttcaacg gcacaaggaa cccggctgtc tttgacaacc ctttgtccat tttcgccttt
      781 gcggccagta ccgcagtcat ggtcttgcca gctccaactg catggttgaa gaaggagcgg
      841 ccgtattcca tgtagtgatg cacggctttc cattgccatg ggtttatctt cagtggcttg
      901 ccattgaagg tcttactgag tcccctcagc tccatttcac cggtagagtg cttcttctcg
      961 taataggcaa actggttgtt atagttctcc ttgacctgat cagccagccc atggtctttg
     1021 acgaattcat tgaacttctc cgtcagcacc cgattccttt cagttgttgt ttctggatca
     1081 ggtattttct ttgctttttc tggatcagaa gttattttgc catccgtgcc tacgaatgtg
     1141 ccttgctgtg gcccctccat cggcttcacc tcgtctgtgc gattcatcca cctcgcaaac
     1201 atccccctgg tgatggactt gccatttgct gacaaggctt ctgtgattaa gttgttatct
     1261 gactgtccga taaactccca gcggacagta gatcctatct gcttctgcac aatagcagat
     1321 ctcatatccc cgcccatcat cagatcggca aacccatata tgatgtcagg aaggctatgc
     1381 acaacgccgg cattgggatc aagactcaca tccttcaact caatgggagg gattgaagcc
     1441 ctgagctcgt tcagtttggc taccgcagca tccaggttga tgggatcaac gccctctccg
     1501 atcatttggt caagggcttc ggttgcgaaa gcaatcttct cgatgatgtt gccaacggca
     1561 aagtcttcgg ttgccatgac atgaagggcg tcccttctgg tgacatccat catgtactcg
     1621 ccagttgctg tcagagcctt ggtgaactcg tactcgtcat tcagcctttc tgccaattct
     1681 ggattcaacc gtaatttgct ggcccggata cgctcaatca ggtgatcaac agcaaagctc
     1741 ttgggtaaac cctctctgct ggcaatggtg ttggtgagat ccacggcaga ggtaaattcc
     1801 tgctgctcgt catacctgac cttcttatcc ccgaataagc cctcccagaa atctggcttc
     1861 atcagtttgc catcctcgtc ttcaacaagg ttggcaatac tggtgatggc atagcttctg
     1921 gcatctgcct tgaattcatc tgtggcggcc agctgcttca ccagatcgga cttcgacaga
     1981 gactccagtc cattcctgag aatgaactcc tggagctctc ccacggctgc tttcctggca
     2041 atgctttgtt cgtgatcgct gagctggtta ttattcgccc gatccctgta ctccagtatc
     2101 ttgtccacgg tctgaagaag atccatcagc tccggagttc ctgccacttc cttatagaag
     2161 gagccggtta cgctacccag caccgcctcg atataattcg ccattttcac ctgctggttc
     2221 aggttgtctg tgacgccctt cagcatggtc atgatctgct tacggcgcat ctgctcaaaa
     2281 ctttcgccca gcttctcctt cctggactgc tttttcttcg taactctgta atcagagttt
     2341 gccattaatc cctcgatgga aatactacct tttacccctc tcagcttctt tgctgagtct
     2401 gctccaaatt ccttttcgaa tccagcatat aacatattgg taagcctatc aggggtgacg
     2461 tttgacttcc tgcccagcac gtcgccttgc tcaccgtgaa taacgatgtg cgtaccaggt
     2521 tgaacattgt ctgctggctt taccatgaaa tccccgacct ttttgatatg aacctttttg
     2581 ttcagctctg aatcgggcat gaaccgcatt tcctgcattt cctgaagtct cgtgctctca
     2641 ggccccatgt ctatttcact gccaaatacc tttggtcccc ggaatgtatg gtgaggcgtc
     2701 aagtgctgcc cctctatcag ctggttcagg tttgtgatga tgtggggctt tgtgctcttg
     2761 ccttccagta tcctgttgga gatatcgtaa ttaacgagcc cgtggataag gtgctgaata
     2821 gctgttgaaa gcgggcccaa aatatcttcg acctgccatg tgccagctat caggctatac
     2881 gcagggttta tgttcgttgc atccgcagct gtgacagtct cggttatctt ggatttgatg
     2941 tcctcctgaa tgagatccca cagtggaaca atggcgtcca tactgatgat ctggccttct
     3001 ccctgctcga agaaagcacg cttcagtctt tcgtctccct ttctctccac aagccccttc
     3061 aggaatacat tggctttcct gaaccactcc tgagaattgg gttcgtggta tgccaacggc
     3121 acacgcctta cctcggactt caggaaatcc tctctgttct ggcgcagttt cgcaaaggtt
     3181 gatactatgg ggaaaagctc ttcctcggcc atattcagtt ttacagccag cgattggaac
     3241 ttcttatagt tggttacatt gaatttcccc tcaaccatct ttctcgcaag cgcagaatct
     3301 gaggtattga ttacgtcttg ggctacagca acagctggaa gatccctgcc aagttcattc
     3361 acaaagccgg agatcactgc ttccgtattg gttggcttcc tgaatatcag gagatccgca
     3421 tcaacagttg tgctggcaga atctttgaaa gtgttggatg gaagcctgat tgcagtaaca
     3481 acatcagctt tggtcgcgaa tatggactga gcgcccttca ccttatcggc tacaccggtc
     3541 ggggcaatga acaccccata tccaccagga gctaacaggt ccagactttc ggcaataaag
     3601 ccgtcatgca aggccatatc tgacatccgg gcattggcat caaacggcac gttggtaatg
     3661 atcaggtcga atttcttgtc gccatgagcc ttcttgaagc ccatcagacc gggagtagat
     3721 actctggtca ccggatcttt atactgtgcg aacgccctgg tcttggggaa cagtagtttc
     3781 gccactttca agcccacgct ctgaggcaga tccacaatga ccgtttccag atcgcctgag
     3841 tccatcttat catgaagtga tgcaggcatg gccatcaact cccttcccat ccccacgccg
     3901 gggatcagta tgctaccgac cttatccaga aaacccatat cggacaggac gccatataca
     3961 cgctggtaca cttccagtgg agtgtagtgc tcgtattccg ttcctgtgga atagctggag
     4021 atattgcccc ggccgctgta tgccatcagc gcacgaacat cgtcagctgt cagttctacc
     4081 ttctctccgg attcaactgc ctcgatgatc tgttctgctt tcaggttggc ggctctccgt
     4141 ttctcaggag tgccgagatc aatgtcccct gtctctatca gcatggcgcc tctgcgagcg
     4201 ttcttgcgtg ttcccacgcc ggtcagaccg tttcctacga tacgaccgcc aactcctgca
     4261 gatgtgcttc ctgatgtggc aggatcttcc tggctctttg gctgtgggcg cactggcgtt
     4321 tcctccggtg tcacctccag ttccggttct tccttctgtt tcagggctgc aaattcagat
     4381 accttaagaa ggttgccctc tgcatcttcc tgatcagtgt aagagtccca ggcttcttcc
     4441 agatactccc gaaccacttc gcccagttcg gacacgatct tctcagccca tgccttgaat
     4501 ttgtagtaac cgagttgcac aaatatctcc gcaagctcca ccagcgcctc gatcaattcc
     4561 gcattgtcct ctgcttcttt tttaggatca gatataatgc cgagtttacc cttatccttc
     4621 agcattttgc gaaaagcggc cagtttctca tttcccaggc gttccagttc agccagctta
     4681 tctcctttgg atgtgtcggg ttcgggttca ggctgcgctt cttctgcagg cgtttcttcg
     4741 ggagcctcct ttttgtcagg tatcacagca tccctgctct ttgtcaggtc tgaaagctca
     4801 aatgcggttt cttccaggta gtctgcggga acattctttt tccgcatgac ctggccaccg
     4861 gcgttcttgc cgaggttgat attgtaggtc ccatcggtat tgacggactc gatcacgccc
     4921 ttcattccct ttgcggttcc gagatagtta tcggcaaacc tgacggataa ccctattttc
     4981 tttggatctg cttttgccgg ctctaccaca ggctcttttg ccaggtactt atttatccca
     5041 gcaacaatgc tgtccagatc agtccagtcc atcttgtcca tgtacggctg catggctgga
     5101 tctgctttca gtaagtcagc tgcgtatgtt ctggctttga agaggttctt atcccattct
     5161 tctgcgggag cctctgtttc ttttggttcc ggcttctgga tcggcaggtc gtcaaatagt
     5221 ccgatggtgg aattctctgc gttcttgacg ttcgcctctc ccttctgctt gaactgcacc
     5281 aactctgtgc gcagtttatt caggagagcc atatcgttag acagcatttc cgtgatgctc
     5341 tgggtgtcca gaccttcatt gatataccgc ttccgcttgg cctcgatatt tttctccagg
     5401 tccttgatgg ctttttcctt ctcggctaca tcagcatcaa actcctgttg gtaggtggac
     5461 ttgctgataa acctctgcag gttcattggc tgagccggat caaacatcag gttattcaca
     5521 gccttatcca caacagcccg tacctgatcg taatttgtgt atctggggtt tttcaggagg
     5581 aaatcaaaca attcgtcctc atgggcattg gtcatttgct cgtatccggc acggagttta
     5641 cctccccagc gagcaatttg cacgacattc tctacctgat cactttggct ctgaccaaac
     5701 tggttaagag ctgtcatgat ctttccggta gggttcagat aggagatatc aatgatcttc
     5761 tgagcattcg ccccttctgc gagctttgca gcctccagga tagcggtctt gctcttgccc
     5821 tgctcccgca tcttccggta cacttttgac cgttctacgt cggtttcctt gcttgccagg
     5881 gtgttggatt caatcgccag cttcagcgcc tcctccttac tgatgccctc aaatattttg
     5941 gcggggatct tgtcaaatcc tgatgcgcct tcatcagcct gcatccggaa gaaggtggaa
     6001 cggctatgac cagacagcac atacagcttc ccgtctttcg ggttcttcca cagccagatc
     6061 gggtccatct tggccatatc gaagccagca cgcatcttat ccactgactg actgctgtaa
     6121 accacaccct tataggtgtt gtcaccttcg gagtcgcggt tctggaattc ccgcat
//
LOCUS       DAOMAX010000156         6162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2039_length_6162_cov_4.665793, whole genome shotgun sequence.
ACCESSION   DAOMAX010000156 DAOMAX010000000
VERSION     DAOMAX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_6162_cov_4.665793"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK742_08630"
     CDS             complement(<1..344)
                     /locus_tag="PK742_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQU76693.1"
                     /translation="MEKDLIIQSTPRGVEMALLEDRQLVELHKESGSSGFRVGDILIG
                     KVKKVSPGLNAVFVDVGFERDAFLHYTDLNPHFRSIVKYTEAALNRRPIPMQSFRKEP
                     EIIKTGKINNVV"
     gene            complement(545..1324)
                     /locus_tag="PK742_08635"
     CDS             complement(545..1324)
                     /locus_tag="PK742_08635"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76694.1"
                     /translation="MKKQFLIPAAAVAVALLLYFGLSNKPPKVTAFDEKRDLLLDSLD
                     LPGEERMHTLQNSLKNTADTAVLGELSHLWYHAGYPDIAADYERKIADLYPSVDGYIR
                     AGRTFFEALPTDTSQTMQVNLVYGARYCYEEALKLDSNSLDASIGLAQVYVQGTNEPM
                     KGIMMLRELDASHPGDPRVNMELGRFSVMSGQYDRAIERFNSVLEKDSLHLQARFLLA
                     ESYLSTGDTLSAVKTLEKAIGLTGDQQMDAQIRQYIDTLTK"
     gene            complement(1329..1625)
                     /locus_tag="PK742_08640"
     CDS             complement(1329..1625)
                     /locus_tag="PK742_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQU76695.1"
                     /translation="MRKADLVNRISEKTGIPKVDVLVSIEAFCKEIKDSLAEGENVYI
                     RGFGSFIVKTRKRKIGRNIKKNVAIEIPEQSIPAFKPAKVFMGKVKNAKPQAEE"
     gene            1710..2768
                     /gene="mutY"
                     /locus_tag="PK742_08645"
     CDS             1710..2768
                     /gene="mutY"
                     /locus_tag="PK742_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQU76696.1"
                     /translation="MNTPKLFPGTFSKLLLHWYETHGRSLPWVGEQDPYRIWLSEVLL
                     QQTRVEQGMPYYLDFIRSYPTVFDLAAAPESEVLKKWEGLGYYNRARNMLATARLISE
                     KGGTFPDTPDELRKLKGIGPYTANAIASFAFGYPAAVVDGNVYRILSRVFGVAESPDL
                     PEGKKRFATLADDVLDRKRPAAFNQAMMDLGSLVCTPVKPDCDHCPLAGICYARKQEM
                     QTSFPVRKKRTQRSERYYHYIIVRKNGKWLIRQRTEQDFWKGLWEFPCLESDRLRTWN
                     LICRQLGWNTSFLQKPEVLGPFRQQLTHRTVHARVFVLQDLPEPFNLWPEAVWVDEEK
                     LSDHAFPVLIRRIFSEIT"
     gene            2805..3239
                     /locus_tag="PK742_08650"
     CDS             2805..3239
                     /locus_tag="PK742_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQU76697.1"
                     /translation="MSGVNKVILVGNLGKDPELRNFEGGNMVANFTLATTEVYRDKNG
                     NKADHTEWHNIAMWGKLADIAGKLLRKGSKVYLEGKIKSRSWEDKEGNKRYVTEIIAD
                     SFTLLDRKSDNGGGYPNDDNYNVNQDSGVTTGVDQADDDLPF"
     gene            3291..3887
                     /locus_tag="PK742_08655"
     CDS             3291..3887
                     /locus_tag="PK742_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76698.1"
                     /translation="MYKSLIFFLLLSLSAVLISCHTDQSPKPAGYPRIHFPTDTGHYV
                     YQPQDCPYTFELPDYMRVEKKEKFFEEDVSGNCWFNLICDTLNATIYLSYKELDQRMS
                     LLNLMEDAYRLTYKHTNKADYIQPRELDNGHGGVGLIYYVGGDAASNIQFFITDTTQH
                     FVRGALYFYAHPNADSLQPVIRFMVDDIDRMLDSWRWQ"
     gene            3929..6034
                     /gene="recG"
                     /locus_tag="PK742_08660"
     CDS             3929..6034
                     /gene="recG"
                     /locus_tag="PK742_08660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQU76699.1"
                     /translation="MSRYLRTPLTYLKGVGENRAKDMAAHMQLQTFGDLLEYFPYRYE
                     DKTTVVRIADIQPDSGAVLLRGTLTHINPAGGVRATRITARLHDSSGSIEVIWFNGIS
                     WLKKFLKEGTTYNVYGRPNLFNKKLSISHPEVELAGQEDQGGAGKLEPVYSVPDKLKS
                     KGFSSKLMGKMTKALLDQLHPTDTPECIPTRVLEAFRLTERYSALNRIHHPASQQEAD
                     QAIRRLKFEELFVDQVRMLRTRTDRQQKFSGIPFTRIDGLFVRFYEEGLPFELTDAQK
                     KVLREIRKDVVSGHQMNRLLQGDVGSGKTLVALMTMLMAVDNDYQACLMAPTEILARQ
                     HMETFTALLAPFDIKVALLTGTVKGKERKEVLAGIADGSIHLLLGTHALIEDTVVFGN
                     LGLVVIDEQHRFGVEQRARMWTKNHQPPHILVMTATPIPRTLSMTYYGDLDVSVIDEL
                     PPGRKPIKTVHYAESMRLRMFGFLREQILEGRQVYIVYPLIDESEKLDLNNLMEGYRG
                     LERDFPRPQFTIGMVHGRMPADDKEFEMKRFVEGKTNILVSTTVIEVGVNVPNATVMV
                     IENAERFGLAQLHQLRGRVGRGAHQSFCILMTGEKLSKDGRTRIKTMTSTNDGFVIAE
                     VDMQLRGPGEIEGTRQSGQPLFKLADLRHDVNVLQAARDAAIEVLDTDPLLEQPGQAG
                     LRRHLDDLQSGEKWWGKVS"
BASE COUNT         1617 a         1460 c         1552 g         1533 t
ORIGIN      
        1 ttgaccacat tgttgatctt gccggtcttg atgatctccg gttccttgcg gaacgactgc
       61 ataggaatgg gcctgcggtt cagggccgct tccgtgtatt tgacgatcga ccggaaatgg
      121 gggttgaggt ctgtgtagtg caggaaggca tcccgttcaa aaccaacgtc aacaaaaaca
      181 gcgttcaggc cgggactgac cttttttact ttgccgatca ggatatcacc aacgcgaaat
      241 ccgctgcttc cgctttcctt atgcaactca acaagctgcc ggtcttccaa tagtgccatt
      301 tcaacccctc ttggggttga ttgaataata agatcttttt ccacgtactc gtgttgtgcc
      361 aacctgtaag gtcagcaatg aataaaccga ggctgaactg aagagaagaa gaagcaggaa
      421 gaatatagcg gataagaaga agggttactt cttcttatga cggttctttc tcagcctttt
      481 tttacgcttg tgagtagcga tcttatgtct tttacgtttt ttaccgcagg gcatgtgaaa
      541 tattttattt cgttaacgtg tcaatatatt gtctgatctg ggcgtccatt tgctgatcac
      601 ccgtcagccc tattgctttt tccagtgttt ttactgccga tagtgtatca cccgtggata
      661 aatagctttc agcaagtaaa aaacgagcct gcaaatgtag agaatctttc tctaaaacag
      721 aattaaatct ttctatggca cggtcatact gcccgctcat gacagaaaac cggcctaatt
      781 ccatgttgac acgggggtcg ccgggatgag aagcatccag ctcacgcagc atcataatgc
      841 ctttcatggg ctcattggtg ccctgtacat agacctgtgc gaggccaata ctggcatcca
      901 ggctgttgct gtccagtttc agggcctctt catagcagta gcgggctcca taaaccaggt
      961 tgacctgcat ggtctggctg gtgtctgtgg gaagggcttc aaaaaaagtg cgtccggccc
     1021 ggatataacc atcaaccgaa gggtagaggt cggcgatctt ccgttcataa tcggccgcaa
     1081 tatcaggata gccggcatgg taccagaggt gcgacagttc ccccaaaacg gcagtgtcag
     1141 cggtattttt caaagaattc tgcaaggtgt gcatccgctc ttcacccggc aggtccaggc
     1201 tgtccagcaa aagatctcgc ttttcatcaa aggcggtcac ctttggtggc ttgttgctga
     1261 ggccgaaata caatagcaag gctactgcta cagcagcagc gggaatcagg aactgctttt
     1321 tcaaaatact attcttctgc ttgtggtttg gcattcttga ccttgcccat gaataccttg
     1381 gcaggcttaa atgccggaat gctttgttca ggtatctcta tcgcaacatt ctttttgatg
     1441 ttcctgccga tcttccgctt gcgggtcttg acaatgaaac ttccaaagcc acggatgtac
     1501 acattttcgc cttctgcaag tgaatctttg atctccttgc aaaaagcttc gatgctgacc
     1561 agcacatcta ccttggggat gcctgttttt tctgatatcc ggttgaccag atctgccttt
     1621 ctcataaaat atcctgttta tttattgtgt tttctttaaa caatatacct cttacaaagt
     1681 tcggtaattt gttgaatttc accgtttaag tgaatacacc caaactgttt cccggcactt
     1741 tcagcaagct gttgctgcat tggtatgaaa cccatggccg cagtctgccc tgggtgggtg
     1801 aacaggaccc ctaccgcatc tggttgagtg aggtactgct gcaacaaacc agggtggaac
     1861 agggtatgcc ctactacctg gactttatac ggtcttatcc cactgtcttt gacctggctg
     1921 ctgcccccga aagtgaagta ctgaagaaat gggaaggact gggttattac aaccgtgccc
     1981 ggaatatgct ggccactgcc agactgatca gcgaaaaagg cggaacattt cctgatacac
     2041 ccgatgaact cagaaaactg aagggaatag gaccctacac agccaatgcc atcgcatcgt
     2101 ttgcattcgg ttatccggct gcggtggtgg acggaaatgt gtaccgcata ctaagccggg
     2161 tattcggggt ggcagaatcg ccggatctgc cggaaggaaa aaaacgattt gctacactgg
     2221 ccgatgatgt actggatcga aaaagacctg cggcattcaa ccaggcgatg atggatctgg
     2281 gcagccttgt gtgcacgcct gtaaaaccgg actgtgatca ttgccccttg gccggtatct
     2341 gttatgcccg gaagcaggaa atgcagactt cttttccggt gcggaaaaaa cgaacccaac
     2401 gcagtgaacg ttactaccat tatataatag tgcggaaaaa cgggaagtgg ctcatacgcc
     2461 agcgaacaga acaggatttc tggaaaggac tttgggagtt tccctgcctg gaaagcgaca
     2521 gactacgcac ctggaacctg atctgcaggc aacttggctg gaatacctct ttcctgcaaa
     2581 aacccgaggt gctggggcct ttccggcaac aactaaccca caggaccgta cacgccaggg
     2641 tatttgtgct ccaagacctg ccggaaccgt tcaacctatg gccggaggcg gtctgggtgg
     2701 atgaagaaaa attatctgac cacgctttcc ccgttttgat caggcgcata ttttctgaaa
     2761 tcacgtaact tagctgcaat taatcatacg ttaaaacaaa ttttatgagt ggagttaaca
     2821 aagtcattct cgtaggtaat ttaggtaagg atcccgagct caggaatttc gaaggtggta
     2881 acatggtggc caactttaca ctggctacaa cagaggttta ccgcgacaag aacggaaaca
     2941 aggctgatca cacagagtgg cacaatattg ccatgtgggg taagctggca gacattgccg
     3001 gaaagctgct cagaaaaggc agcaaggtgt acctggaagg taagatcaag agtcgttcct
     3061 gggaagacaa ggaaggcaac aagcgttatg taacggagat cattgcagac agctttaccc
     3121 tgctggatcg taagagcgat aacggtggcg gatatccgaa cgatgacaac tataatgtga
     3181 atcaggacag cggcgtcact actggtgttg accaggctga tgacgacctg ccattctgat
     3241 cgaacagtta tccaacagcc ggggcctgac cccggcttat ttttttatat atgtacaaaa
     3301 gtctgatctt tttcctgttg ctgagtttgt cggccgttct gatctcctgc catacggatc
     3361 agtcgccaaa acctgcagga tacccgcgta tccacttccc gacagatacc ggacattatg
     3421 tctatcagcc acaggactgc ccgtacactt ttgaattacc cgattatatg cgggtggaaa
     3481 agaaagaaaa attttttgag gaagatgtgt cgggtaactg ctggttcaat ctcatctgcg
     3541 ataccctgaa tgccaccatc tacctgagtt ataaagaact tgatcagcgc atgtctcttc
     3601 tcaacctgat ggaagatgct tatcgcctca cctataagca taccaataag gccgattata
     3661 tccagccacg ggagctggat aatggtcatg gtggggttgg acttatctat tatgtgggcg
     3721 gagatgctgc cagtaatatc cagttcttta tcacagacac cacgcaacat tttgttcgcg
     3781 gtgcactcta cttctatgcc catccgaatg cagactcact gcagcctgtg atccggttca
     3841 tggtagatga tattgacagg atgctggaca gctggcgctg gcagtaagaa ccatcgcccg
     3901 aaggcattgt gtatttttga gaccggccat gagtcgatat ctccgaacac ccctgacata
     3961 tctgaaaggc gttggtgaaa accgggccaa agacatggct gcacatatgc agctgcaaac
     4021 tttcggcgat ctgctggaat acttccctta tcgctatgaa gacaagacca ccgtggtgcg
     4081 cattgcagat atccaaccag acagtggtgc tgtattgctg agaggaacac tcacccatat
     4141 caacccggct ggtggtgttc gtgccaccag gatcaccgcc agattgcatg acagcagcgg
     4201 atcaattgaa gtcatctggt tcaacggcat ctcctggctt aaaaaatttc tgaaagaagg
     4261 tactacctat aatgtatatg gaagacccaa tctcttcaat aagaagctga gcatatcaca
     4321 cccggaagtg gagctggccg ggcaggaaga tcagggaggc gccggaaaac ttgaaccggt
     4381 gtactcggtt ccggataagc tgaagtcgaa aggattcagc agtaaactga tgggtaaaat
     4441 gaccaaggca ttgctggatc aattgcaccc taccgataca cccgaatgta ttcccacccg
     4501 ggtactggaa gcatttcgtc tgaccgaacg atacagcgca ctgaacagga tccaccatcc
     4561 cgccagtcag caggaagcag accaggctat tcgccgtctc aaatttgaag agctgttcgt
     4621 agatcaggtg cgtatgctca gaaccagaac agaccggcag caaaaattca gtggcatacc
     4681 ctttaccaga attgatggat tgttcgtgcg cttctatgaa gaaggtcttc cctttgaact
     4741 gaccgatgcg cagaaaaaag tattgagaga gatcagaaaa gatgtggtga gcggccatca
     4801 gatgaaccgg ctgttgcagg gcgatgtggg aagcggtaaa acgctggtgg cactgatgac
     4861 gatgttgatg gcggttgata atgattacca ggcctgcctg atggcgccta cagagatact
     4921 ggctcgtcag catatggaaa ccttcactgc cttgctggca ccatttgata taaaagtggc
     4981 tctgctcaca ggtacggtga aggggaagga aagaaaagaa gtactggccg gtatagccga
     5041 tggcagtatc cacctgctgc tgggtaccca tgcactcata gaagatactg tggtgttcgg
     5101 taacctcggt ctggtggtca ttgatgaaca gcatcgtttt ggtgtggaac aacgtgcccg
     5161 tatgtggacc aagaatcacc agcctccgca catacttgtc atgacagcta cccccattcc
     5221 gagaacattg tctatgacct attacggcga tctggatgtg tctgtcatag atgaactgcc
     5281 tcccggccgg aagccgatca aaacagttca ctatgctgaa agcatgaggc tgcgcatgtt
     5341 cggttttttg cgtgaacaga tcctggaagg aagacaggtt tatattgtct atccgctgat
     5401 agacgaatca gaaaagctgg acctgaataa cctgatggaa gggtatcgcg ggctggaacg
     5461 tgattttccc cgaccacaat tcaccattgg tatggtacac ggccgaatgc ctgcggatga
     5521 taaggaattc gagatgaaaa gattcgtgga aggcaagacc aatattctgg tctctactac
     5581 cgtcattgaa gtaggtgtga atgtgccgaa cgccactgtc atggtcatag aaaatgccga
     5641 gcggttcgga cttgcacagc tccatcagtt gcggggaagg gttggtcgtg gtgcgcatca
     5701 gtctttttgt atcctgatga ctggtgaaaa actttcaaag gatggacgca cccgcatcaa
     5761 aacaatgacc tctaccaacg acggttttgt cattgctgag gtggacatgc agttgcgtgg
     5821 ccccggcgag atcgaaggca ccagacaaag cggacagccg ctgttcaaac tggcagacct
     5881 tcgtcatgat gtcaatgtgc tgcaggccgc ccgcgatgca gccatagaag ttctggacac
     5941 cgatccgctg ctggaacaac ccggtcaagc cggccttcgt cgccacctgg atgacctgca
     6001 gtccggcgaa aaatggtggg gaaaggtttc ctgaacaaag acctgcccgg gtgtatatcg
     6061 cctgaaaccc ttgtgattag tgcattttca ggacacccta aagaatattt aataaaaaat
     6121 gtaaatagat aaattattgt ttatcaatta gttgaaacat ga
//
LOCUS       DAOMAX010000157         6126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2058_length_6126_cov_4.909735, whole genome shotgun sequence.
ACCESSION   DAOMAX010000157 DAOMAX010000000
VERSION     DAOMAX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2058_length_6126_cov_4.909735"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..915)
                     /locus_tag="PK742_08665"
     CDS             complement(307..915)
                     /locus_tag="PK742_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76700.1"
                     /translation="MNRIWYVLLCSSLLLAGISAKAQNNEVCPDQASLVNETMNRITE
                     VIVHDVFTPPVASRIYAYTAIAGYSAYHFIQGETPFTVKLNEFPELPLSSPNQPYCAS
                     LAMSSAMFETAKALLFSEAMVDSSFADFTAIYFEDKLDEKTRQTSINYGRFVASQVIE
                     WAGQDNYRETRSLEPCMGELGRPMDSRASISLRTRPTASLTY"
     gene            complement(918..4211)
                     /locus_tag="PK742_08670"
     CDS             complement(918..4211)
                     /locus_tag="PK742_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQU76701.1"
                     /translation="MLRIRPFYKLFFFLLVLIPAILSRAQYQPPQFNLTDAGLTGISF
                     SNDLQEDEERNVLQYQYFYNGGGVCAGDVNNDGLCDLFFTGNDVSNRLYINQGNWYFQ
                     DVTAKAGLTSAGWSTGAVMADVNDDGWLDIYVCRSGKYDAENRKNLLFINQRNNTFKE
                     EAARYGLDDPAQSTMAAFFDYDRDGDLDMFLLNHSVTQYSNFNVAELRSKRDPMAGNK
                     LYRNDKGHFTDVSDAAGIYGNPINFGLGVAISDFNNDGWPDIYCTSDYQEQDYLYYNN
                     GDGTFTQVMQYAMAHTSLFSMGTDWGDINNDGLSDLIVADMLPASNKRQKMLKGPLKF
                     DAYQLAVDYGFHHQLMRNTLQLNRANGTFSDIGMMAGVAATDWSWAPLLADLDNDGWQ
                     DLFVTNGYLRDFTNMDFLKYTYNEELDKASSRGEEVDMLHLVHQMPSVKLPNAVYHNN
                     HDLTFSNKTRSWDMNQPGFSNGAVYADLDNDGDLDLVVNNLNDTAFVYRNNGGGGYLT
                     FTFDGPAGNRFGVGTKVTITGKDLYIVRENFPTRGYQSAVESKLYAGLGSSYLVDVQI
                     DWPDGKRQVLKQVKANSTLTCKWKDADDNVPSIARTFGQPLMEESATPVLPATYINNE
                     FIDLKREPLLTFGLSDTGPRMAIADVNGDGKEDVFFCAAKGQQSQLYIQQNGQLVLSS
                     DQPFNTANPQDMSDAALFDADGDGDNDLFVTFGGNEAELSTGYVPQLLLNDGQGNFIP
                     AKNAFPEIHISASCVRPTDIDLDGDQDVFIGAACLPGRYPQSGSSYILRNEGGRFTDV
                     TDQYSPLLRQAGMVTDARWADLNGDKLPDLLFTGYWMPVRIFLQGEEGMEEQVDFEGM
                     SGASGWFSCLIPGDVDGDGDLDFLAGNNGLNSPFKASSTKPLYLYAKDFDNNGTIDPI
                     IAGYKGDTCYPLVSRDDLLDQVNGLKKRFVRYAQYANATVQDIFPNEDWSSVWRLEAT
                     NLSTCLFINDGDGQFHTDSLPYEAQMFPVRCATFADVNGDGKTDLILAGNRFNVRPDV
                     GRMDAGQGLLLLQTPGGWSAAENTESGLYLPEEMTDMKWLTLNLERYLLVSGIHQQVR
                     SYHIRKNVK"
     gene            4378..>6126
                     /locus_tag="PK742_08675"
     CDS             4378..>6126
                     /locus_tag="PK742_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQU76702.1"
                     /translation="MNIIYSRIKKHTMLALMMLPAWVMAQTATLEGTVTDSNGEPLIG
                     ATVLLEGTTIGGITDFDGKYEVTNIPVGTYNVAYSFVGYKKVTESVTLTVGQRVSRNI
                     VLEDDANILEEAVVIGYGTTQTKDLTGSVVAIDTKDFQKGNFATPEQLVTGKIAGVQI
                     TSNSGMPGAGSRIRVRGGTSLNASNDPLIVIDGVPVDNGGISGAGNPLSLLNPDDIET
                     FTVLKDASAAAIYGSRAANGVIIITTKKGAASNKLNVNFSTNNSISQVTGFVDVLSAD
                     QFRDTVTAHGNATQIGLLGESSTDWQKEIYQTGISTDNNLSLSGGVKNLPYRLGLEYN
                     HTEGILKTSQLDRVGTTLNLSPELLNKHLKVDANAKYYNVQNVFANQGAIGSAVTFDP
                     SQTVMSGDTVYGGYFEWISGNGNPNALAPRNPVGLLDQRNDESSVNRFIGNILLDYGT
                     HFLPDLHLFVNAGIDMADASGSVFVPATAASDFLRGGVNNVYEQSKRNRVLETYFNYK
                     HDLKSIKSSFDVTGGYSFQYWSTESPAQPDLNVAGDTITPPGIPFFTDNALMSFYGRL
                     NYNLAEKYLITATLRED"
BASE COUNT         1463 a         1574 c         1536 g         1553 t
ORIGIN      
        1 agattattga tcaaacttca taataactga catgcaaccg gatacgaact tcatcacctg
       61 gtttcgcgcc gtcacacctt acatccaact cttccggaac aagatctttg tgatcgcctt
      121 tggcggcgat gtcgtggctg atgaaaaatt tgtgtcactg gtgcatgatt tcaatttgct
      181 cgccagcctt ggcatccgcc tcgtgctggt gcacggcgca cgcccccaga ttgaatcccg
      241 tctgcaggct gatgcacacg aaatttctta tgtgcagggg atgcgcgtta ccgatgcaaa
      301 tgcgcttcag tacgtcaagg aagcagttgg ccgtgtgcgt agcgagatcg aagcgcggct
      361 gtccatgggc ctgcccaatt cacccatgca gggctccagg ctgcgggttt cccggtagtt
      421 gtcctggccg gcccattcaa tgacctggct ggctacaaag cggccatagt tgatggatgt
      481 ctgtctggtc ttttcgtcca gcttatcttc aaaatatatg gccgtgaaat cagcaaaaga
      541 agaatctacc atggcctcag agaacaacag cgcctttgct gtttcaaaca tggcgctgga
      601 catggccagt gatgcacaat agggttggtt gggagatgac agcggcagtt caggaaactc
      661 attcagtttc acggtaaatg gcgtttcgcc ctggatgaag tgatatgctg aataacccgc
      721 aatggccgta taggcataaa tgcggctggc tacaggaggt gtgaacacat cgtggacgat
      781 gacctccgta atgcggttca tggtttcatt gaccagacta gcctgatcgg ggcatacttc
      841 attgttttgt gcctttgcag aaatacctgc cagaagcagg ctgctgcaaa gcaggacata
      901 ccagattcga ttcatgatta cttgacattt tttctgatgt gataagaccg cacctgctgg
      961 tgtatgcccg aaaccagcag atatcgttcc agattcaaag ttaaccattt catatcggtc
     1021 atttcttccg gcaggtacag cccgctttct gtgttttctg ctgcgctcca gccaccgggt
     1081 gtttgcaaca gcagcagccc ctgcccggca tccatccggc ccacgtcggg tcgcacattg
     1141 aagcggtttc ctgccaggat caaatctgtt tttccatctc cgttcacatc agcaaatgtg
     1201 gcgcaacgaa ccggaaacat ctgtgcttca taaggaaggc tgtctgtgtg aaactgtccg
     1261 tcgccgtcat tgatgaacag gcaggtggac aggttagtgg cctccagtcg ccatacagac
     1321 gaccagtctt cgttcgggaa gatgtcctgt acggtggcat tggcatattg cgcatagcgg
     1381 acgaagcgct ttttcaatcc gttcacctga tccagcaggt catctctcga taccagcgga
     1441 taacaggtat cacctttata tccggcgatg atggggtcta tagttccgtt gttatcgaaa
     1501 tccttcgcat agagatacag tggctttgtg gaactggctt tgaatggcga gttgagtcca
     1561 ttgtttcctg ccaggaaatc aagatcgcca tcgccatcca catcgcccgg tatcaggcag
     1621 ctgaaccatc cggatgctcc gctcatgcct tcaaagtcca cctgctcttc catgccttct
     1681 tcgccctgca ggaaaatgcg cacgggcatc cagtatccgg taaacaacag gtcaggaagt
     1741 ttgtcaccat tcagatctgc ccagcgggca tcggtcacca tgcctgcctg acgcagcagc
     1801 ggactgtact gatcggtcac gtctgtaaac ctgccgccct catttcgcag aatataactg
     1861 cttccgcttt gcggatacct tcccggcagg catgcggcac ctatgaagac atcctgatcg
     1921 ccgtccaggt cgatatcagt cggacgcaca caggaagcac ttatatgaat ttcaggaaat
     1981 gcattctttg caggaataaa gtttccttgt ccgtcgttga gcagaagttg cgggacatat
     2041 ccggtgctta attctgcttc gttgccgcca aaggtgacga acaggtcatt gtcgccatcg
     2101 ccatctgcat caaagagtgc ggcatcactc atgtcctgcg gatttgcagt gttgaaaggc
     2161 tggtcagatg acaacaccag ctgtccgttc tgttgtatat acagctggct ttgctgtccc
     2221 ttcgccgcac agaagaagac gtcttccttt ccgtcgccat tcacatcggc aatagccatt
     2281 cgcggaccgg tatcactcaa accaaaggtg agtagtggct ctcttttcag gtcgatgaac
     2341 tcgttgttga tatacgtggc gggtagcacc ggagtagccg attcttccat caggggttgg
     2401 ccgaaagttc tggcaataga ggggacgtta tcgtcggcat ccttccactt acaggtcagt
     2461 gtgctgttgg ctttgacctg cttcaaaacc tgcctttttc catcaggcca gtctatctgc
     2521 acatccacca ggtaactgct tcctaatcct gcgtaaagtt tactttccac cgccgactga
     2581 taccctctgg tgggaaagtt ctctctgaca atatagaggt cttttccggt gatggtcacc
     2641 ttggtgccca caccaaaacg gttgccggcc ggtccgtcaa aagtgaaggt gagatatcct
     2701 ccgcctccgt tgtttcggta cacaaatgct gtgtcgttca ggttgttcac cacgaggtcg
     2761 aggtcgccgt cgttatccag atctgcatag accgcaccat tggaaaatcc gggctggttc
     2821 atatcccatg atcttgtctt gttgctgaac gtaaggtcat ggttgttgtg atacaccgca
     2881 ttcggcagtt tcaccgaggg catctggtgc accaggtgca gcatgtccac ttcttcgcca
     2941 cgggaagacg ctttatccag ctcttcatta taggtgtact tcagaaagtc catgttcgtg
     3001 aagtcgcgga gatatccgtt cgtcacaaag agatcctgcc agccatcgtt gtccagatct
     3061 gccagcagtg gcgcccagct ccagtcggtt gctgccaccc cggccatcat accgatatca
     3121 ctgaaagtac cgttggcccg gttcagctgg agggtatttc gcatgagctg gtggtggaaa
     3181 ccatagtcaa cggccagctg ataagcatca aatttgagag ggcctttgag catcttctgg
     3241 cgcttgttgg aagccggcaa catatcagcc acgatcagat ctgacaagcc atcgttgttg
     3301 atatcgcccc aatcggttcc catactgaac agagaagtat gtgccatggc atactgcatg
     3361 acctgggtaa aggtgccgtc accattatta taatagaggt agtcctgctc ctggtagtcg
     3421 ctggtgcaat agatatcagg ccagccatcg ttgttgaaat cactgatggc cacccctaat
     3481 ccaaaattga tcgggttgcc gtaaatacct gcggcatcgc tgacatcggt aaaatgcccc
     3541 ttatcgttcc ggtacaattt gtttcctgcc atggggtcac gcttactgcg caattcagcc
     3601 acattgaaat tactgtactg ggtaaccgag tggttgagca ggaacatatc gaggtcgcca
     3661 tcccggtcgt agtcaaagaa ggccgccatg gtagattgtg cgggatcatc caggccatat
     3721 ctggcggcct cttctttaaa ggtgttgttt ctttggttga taaacagcag gttctttctg
     3781 ttctctgcat catattttcc ggaacggcac acatagatat cgagccagcc atcgtcgttc
     3841 acgtcggcca tcacagctcc ggtgctccat ccggcagagg tcagtcctgc tttggcagta
     3901 acatcctgaa aataccagtt tccctgattg atatacaagc ggttgctgac atcgttgccg
     3961 gtgaagaaca gatcgcagag accatcgttg ttgacatcgc ctgcacagac acctccaccg
     4021 ttatagaagt actggtattg caacacattg cgctcttcat cttcttgcag gtcattgctg
     4081 aaagagatcc ctgtcagacc ggcgtctgtc aggttgaatt gcggtggttg atactgggcc
     4141 cgggaaagaa tggcaggaat caagaccaac aggaaaaaga atagcttata gaatggtctt
     4201 atgcgcaaca tacttgcaaa gatattttga atcaaccagc taaatgatta aggaatagtt
     4261 aatcaaaatt ggaacatttt gacataccgg tagctgtgtg aaaaaatttt taattttcgg
     4321 tgctttttct gaagctgtcg gaaaggcata tttcaatcat taaaaccact ttatcgtatg
     4381 aacattattt actcacgcat caaaaagcac accatgcttg ctttgatgat gttacccgcc
     4441 tgggtgatgg cacaaaccgc caccctggaa gggaccgtca cagacagcaa tggcgagccg
     4501 ctgatcggtg caaccgttct cctggaaggc accaccattg gaggtatcac agactttgat
     4561 ggcaagtatg aggtcaccaa tattccggtg ggcacataca atgtggccta tagttttgtg
     4621 ggatacaaga aagtaacgga gagtgtgacc ctgaccgtcg gacagcgtgt cagtcgcaac
     4681 atcgttttgg aagatgatgc caacatcctg gaagaagctg tggtgatcgg ttatggtacc
     4741 acacagacga aagacctgac gggttcagtg gttgccatcg acaccaagga ttttcaaaag
     4801 ggcaacttcg ccacacccga acaactggtg acaggcaaga tcgcgggggt gcagatcact
     4861 tccaacagtg gtatgcccgg tgcaggtagt cgtatccgtg ttcgcggagg tacttctctg
     4921 aatgccagca atgatccgct cattgtgatc gatggcgtgc cggttgataa cggaggcatt
     4981 agtggcgcag gtaacccttt gtcactgttg aacccggatg atatcgagac cttcaccgta
     5041 ctgaaagatg cttcagcagc ggccatctac ggtagtcgtg ccgcaaacgg tgtcatcatc
     5101 atcactacta aaaaaggtgc ggcctctaac aaactcaatg tcaacttcag caccaacaac
     5161 tccatcagcc aggttaccgg ctttgtggat gtgctgagtg ctgatcagtt cagagatact
     5221 gttaccgcac acggcaatgc cacccagatc ggtttgctgg gtgagtcttc cacagactgg
     5281 cagaaagaga tctaccagac cggcatcagc acagataata acctgtcgtt gtctggtgga
     5341 gtcaagaatc tgccctatcg cctggggctg gaatacaacc atacggaagg tattctgaag
     5401 acctcgcagc tggatcgtgt gggtactacg ctgaacctca gtccggagtt gctgaacaag
     5461 cacctgaagg tagatgccaa tgcgaaatac tataatgtac agaacgtatt tgccaaccag
     5521 ggtgccatcg gttctgccgt caccttcgat cccagccaga ccgtaatgtc tggcgacact
     5581 gtttacggtg gctactttga gtggatcagc ggcaatggca atccgaatgc actggcaccc
     5641 cgtaatcccg tgggtttgct tgaccagcga aacgacgaga gcagtgtgaa ccgctttatc
     5701 ggtaacatcc tgctggatta cggcacccac ttcctgcctg atcttcacct gttcgtaaat
     5761 gccggtatcg atatggcaga tgcatccggt tccgtttttg ttcctgccac cgcagcatca
     5821 gatttcctgc gtggtggtgt gaacaatgtg tatgaacaat ccaagcgcaa ccgcgtactg
     5881 gaaacatatt tcaactataa gcacgatctg aaatccatca agagcagttt tgatgtgacc
     5941 ggtggttatt cattccagta ctggagtaca gagagccctg cacaaccgga tctgaacgtg
     6001 gcgggcgata ccattacgcc tcccggtatt ccgttcttta ctgataatgc gctgatgtca
     6061 ttctacggaa ggctgaacta caacctggct gagaaatacc tgatcaccgc taccctgcgg
     6121 gaagat
//
LOCUS       DAOMAX010000158         6101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2074_length_6101_cov_4.423090, whole genome shotgun sequence.
ACCESSION   DAOMAX010000158 DAOMAX010000000
VERSION     DAOMAX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_6101_cov_4.423090"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2204
                     /gene="carB"
                     /locus_tag="PK742_08680"
     CDS             <1..2204
                     /gene="carB"
                     /locus_tag="PK742_08680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQU76703.1"
                     /translation="VEKAVLGWKEYELELLRDGADNVCIICVVENLDPMGVHTGDSIT
                     VAPAMTLSDTCYQEMRNMAIQMMRAMGNFAGGCNVQFAVDPHTEDIISIEINPRVSRS
                     SALASKATGYPIAKVAAKLAIGYNLDEIKNMVTKSTSAYFEPALDYVIVKMPRWNFDK
                     FQGADDTLGLQMKSVGEVMAIGRSFNEALQKACQSLENNKIGLDGEVGFYKSSQEVIE
                     GLGRPQWDRIFNIKKAMDLGVPLKTIQKQTLIDRWFLMQIQNLSKLEKELRRYTLQNI
                     PADMMINLKQNGYSDLQIANILGNTTEEEVYEYRRALGIRRTYKLVDTCSAEFESKTP
                     YYYSTFDQENESVKSDKKKIFVLGSGPNRIGQGIEFDYCCVHGILAIKEYGYEAIMVN
                     CNPETVSTDFDIADKLYFEPVFWEHLNELLDFEQPDGVIVQLGGQTALKLSEKIRNKG
                     IPIIGTSFDDMDMAEDRGRFSDLLKELEIPYPEYGSATTAEEALEVANRIGYPVLVRP
                     SYVLGGQRMRIVINDDELERSVIGILKHFPDNRILIDHFLDRAEECEIDAICDGEDVH
                     IMGIMEHIEPAGIHSGDSSAVLPPFSLSQNVLDQMSEFTKKLAHALNIRGLINIQFAV
                     KNEKVYVIEANPRASRTTPFIAKAYQIPYLNIATKVMLGTHKLKDFSFDKKLNGYAIK
                     EPVFSFEKFPNVNKELGPEMKSTGEAIRFIEDLQDPFFRHLYKEKSMYLSR"
     gene            2207..2356
                     /locus_tag="PK742_08685"
     CDS             2207..2356
                     /locus_tag="PK742_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76704.1"
                     /translation="MSDVTIIILVIVFWRLVIGPAIKGFRYPGNSRKKQDEIPYSDYE
                     EVDRK"
     gene            complement(2360..3643)
                     /locus_tag="PK742_08690"
     CDS             complement(2360..3643)
                     /locus_tag="PK742_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQU76705.1"
                     /translation="MRLFLCLFIGTLFSTAARSQFDLGLELRPRMEFRNGYRFPSDTS
                     SKDAFFISQRTRLNLDYADTIITAKLSIQDVRVWGDQPVFSDDAGLSIAAAWVQWHFA
                     RDWYLRTGRQELSFGDKRLIGPAEWRQQARSHDALLLLYQKKVWLFQLAGAFNQNAER
                     LSSTSYGISQYKALGVFQAKRYSGPLKGFFLVIADGTQNLYAGTDEMDWRFTSGGSWS
                     YPIGLFTFQAGGYSQNGVRFVDGRSLFAYMLNGSVGYRHNNWSFTLGYDILSGNDSIG
                     SPDYRAFDNLYATRNAFYGRMDYYYSFPGDMKEAGLHDAWLLVDYKVSYRHQLHGGLH
                     YFQLDKPAFDPDSFEYAGKDLGQELDLWYSFQARHDIQLELGQCFYLPGEDLPLVKGG
                     SPDLLNTWSYFTIRFTPTLFTTRTPTEELILETVE"
     gene            complement(3643..4809)
                     /locus_tag="PK742_08695"
     CDS             complement(3643..4809)
                     /locus_tag="PK742_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQU76706.1"
                     /translation="MATILTFMDTEIWGNTLRQYAWLLGVILVVIIFNKYISNFISRL
                     LFQLLKRSRFKHSGQLFLKLLLQPLEFILVLHTIYIGFQMLDAPEFFQFKFIGATLHQ
                     YAYGLYTLLFIVSTGWLFSRFADFIITVMNEKAQLTPDPSDDQVVSFLKDVFKVVIWT
                     TVVLCVLAFVFHVNVTSLVAGAGIAGIAIAFAAQETLQNIFGSISLFAEKPFLVGDLV
                     ELDGITGTVDKVGFRSTRLRALDKSFQTIPNRNIVSNRIINLSRRTSRRVQFLLGLEY
                     GTKAEAITAIVSEIDAYTTQHPVTNEPSVVHFYGFGASSLDIWVEIYFEYLEWDDFLR
                     KRQEIMLGIMDIVEKHGGSFAFPSQTLYMRQDVPKEPTKKSADISSDIHHPKGE"
     gene            4913..6070
                     /locus_tag="PK742_08700"
     CDS             4913..6070
                     /locus_tag="PK742_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQU76707.1"
                     /translation="MINKLLTPVRTKGLLDITGSPLQMGRCMDIHTGEAPDTYEKDIA
                     IITVNEQRGGDPARSSNKLDAIRKHFYSLINPHDNLRLIDAGEVLLGETPEETTQNLK
                     MVVEFFLAQRIIPVIIGGSQDLSIGQYKAYEFLGQAINLAVIDERIDIDHADDAPPRQ
                     TWLLDIFSHEPNYLFNYSALACQGHFVNNEIFHTIDHLYFDTCRLGRLRDNFQLAEPE
                     LRDADMVSFDLSAIKASDAMGVLDLSPNGVNSEEACQLMRYAGMSDKVSSMGIYNYLH
                     EGDNQELTAQLVAQMIWYFVDGFYNRKHDYPITDESDFMKYIVNLSENEYDLTFWKSK
                     KSGRWWLEVPFTVDTRYERQQLVPCAYADYEEAMKGTLPDKWLRMFEKLSA"
BASE COUNT         1658 a         1581 c         1513 g         1349 t
ORIGIN      
        1 tggtggaaaa agcggtgctg ggatggaaag aatacgaact ggaattgctt cgcgatggtg
       61 ccgataacgt ctgtatcatc tgcgtggtgg agaacctcga cccgatgggt gtccatacgg
      121 gcgatagcat cactgtggca cctgccatga ccctcagcga tacctgctac caggagatgc
      181 gtaacatggc catccagatg atgcgtgcca tgggcaactt cgctggtggt tgcaatgtac
      241 aattcgcagt ggacccgcac accgaagaca tcatctccat cgagatcaac cccagggtgt
      301 cccgttcgtc tgcactcgct tccaaagcca ccggataccc catcgcaaaa gtggctgcga
      361 aactggcaat cggatacaac ctcgatgaga tcaagaacat ggtgaccaag agcacctccg
      421 cctatttcga acctgcgctg gactatgtca ttgtaaaaat gccccgatgg aattttgata
      481 agttccaggg tgcagatgat acccttggcc tgcagatgaa aagtgtgggg gaagtgatgg
      541 ccatcggaag aagcttcaac gaagcccttc agaaggcctg ccagagcctg gagaacaata
      601 agatcggact ggacggagag gtaggattct acaagagttc acaagaggtc atcgaaggcc
      661 tcggaagacc acagtgggac cgcatcttca acatcaagaa ggccatggac ctgggtgtac
      721 cactcaagac catccagaag cagaccctca ttgaccgatg gttcctgatg cagatccaga
      781 acctgtcaaa actggaaaag gaactgcgcc gctataccct gcagaacatt cctgcagata
      841 tgatgatcaa cctgaaacag aacggttatt cagatctgca gatcgccaac atactgggca
      901 acaccaccga agaagaggtg tatgaatacc gcagggcact gggcatccgt cgcacctata
      961 aactggtgga cacctgctcg gcagagttcg aatcgaagac accctattac tacagcacct
     1021 ttgatcagga gaacgaaagt gtgaagagcg ataagaaaaa gatcttcgtg ctgggctccg
     1081 gccccaaccg catcgggcag ggtatagaat tcgactactg ctgcgtgcat ggcatcctgg
     1141 ccatcaagga atatggttac gaggccatca tggtgaactg taaccctgaa accgtttcca
     1201 ctgacttcga cattgcagac aaactctact ttgagccggt attctgggaa cacctgaatg
     1261 aactgctcga cttcgaacaa cccgatggag tgatcgtaca gttaggcgga caaaccgctt
     1321 tgaaactgtc tgagaagatc cgcaacaaag gcatccccat catcggcacc agtttcgacg
     1381 acatggatat ggcggaagac cggggacgtt tctccgacct gctgaaagaa ctggaaatac
     1441 cctatccgga atatggcagt gccaccaccg ccgaagaagc actggaagtg gccaaccgca
     1501 taggctaccc ggtgctggta agacccagtt atgtactggg tggtcagcgg atgcgcatcg
     1561 tgatcaacga cgatgaactg gaacgcagcg tgatcggaat cctcaagcac ttccccgaca
     1621 accgcattct cattgaccac ttcctggacc gtgcggaaga gtgtgagata gacgccatct
     1681 gtgacggaga agacgtgcac atcatgggca tcatggaaca tatcgaaccg gcgggtatcc
     1741 actctggtga ttccagtgca gtattgcctc cgttcagtct gagtcagaat gtgctggacc
     1801 agatgtcgga atttacaaag aaactcgccc acgcactgaa catccgcgga ctcatcaaca
     1861 tccagttcgc agtgaagaat gagaaggtgt atgtgatcga agcgaatccg cgtgccagtc
     1921 gtaccacgcc gttcatcgcc aaggcttatc agattcccta tctgaacatt gccaccaaag
     1981 tgatgctggg tacgcacaag ctgaaagact tcagcttcga caagaagctg aacggatacg
     2041 ccatcaagga accggtgttc tcctttgaaa aattcccgaa cgtgaacaag gagctcggtc
     2101 ctgaaatgaa atcgaccggt gaggccatcc gcttcattga agacctgcag gatccgttct
     2161 tccgtcacct gtacaaggag aaatccatgt acctgagccg ctaagcatga gtgatgttac
     2221 catcatcata ctggtcattg tattctggcg attggtgatc ggtccggcca tcaaaggatt
     2281 ccggtatcct ggcaattcca ggaagaaaca ggacgaaata ccgtattctg attacgaaga
     2341 agtggaccgg aaataactcc tactccactg tttccagtat gagctcttca gtgggagtgc
     2401 gggtggtaaa cagcgtaggt gtgaatctga tcgtgaaata actccaggta ttcaacagat
     2461 caggtgagcc gcctttgacc aacggcaggt cttcgcccgg caaataaaaa cactgtccca
     2521 attccagttg aatatcatgt ctggcctgga aagaatacca gagatccagt tcctgaccga
     2581 gatccttacc tgcatattca aaggaatccg gatcgaatgc cggcttatcc agctgaaaat
     2641 agtggagtcc gccatgcagt tgatgcctgt aagaaacttt gtaatctacc agtagccagg
     2701 catcatgcaa tccggcttct ttcatatcgc cagggaaact gtagtaataa tccatccgtc
     2761 cgtagaaagc attccgggta gcatacaggt tatcgaaggc acgataatcc ggactgccga
     2821 tagaatcatt tccactcagt atatcatacc ctaaggtgaa actccagttg ttgtgccgat
     2881 acccgacaga accattcagc atgtaagcaa agaggctgcg tccatccaca aagcgcacac
     2941 cgttctggct gtaaccaccg gcctgaaaag tgaacaaacc aatagggtat gaccaggaac
     3001 ctccggaagt gaagcgccag tccatttcat ctgtgccggc atacagattc tgggtgccgt
     3061 ctgcaataac cagaaagaat cctttcaaag gtccgctgta acgctttgcc tgaaagacac
     3121 ccagcgcttt atactggctg atgccgtaag aagttgagct cagtctttct gcattctggt
     3181 tgaatgctcc tgccaattga aacagccata cttttttttg atagagcaat agcagtgcat
     3241 cgtgactgcg ggcctgttgt ctccactcgg cgggaccgat cagtcgttta tcaccaaagg
     3301 ataactcctg tctgccggtg cgcagatacc agtcacgggc aaaatgccac tggacccagg
     3361 ccgccgcaat gctcagacct gcatcatcag aaaagacagg ctgatctccc cacacacgca
     3421 cgtcctgtat gctgagtttg gcagtgatga tcgtatctgc ataatccaga ttcaaccggg
     3481 tgcgctgcga aatgaaaaag gcgtctttgc tgctggtgtc agatggaaaa cgatatccgt
     3541 tgcgaaactc cattcggggc cgtaattcca gaccgaggtc aaactggctg cgagccgctg
     3601 tggagaatag ggtgccgata aacagacaca ggaataaacg catcattcac ctttaggatg
     3661 gtgaatatcg gaagaaatgt ctgcactttt ttttgtgggt tccttcggca cgtcttgtcg
     3721 catatacagg gtctgagacg ggaacgcgaa acttccgcca tgtttttcta caatatccat
     3781 gatacccagc atgatctcct gtcgtttgcg caggaaatca tcccactcca ggtattcaaa
     3841 atagatctcc acccagatat caagtgagct ggcaccgaat ccgtaaaagt gtacgaccga
     3901 tggttcattg gtaacagggt gctgggtggt gtatgcatct atctcactta cgatcgcggt
     3961 gatggcctct gcttttgtgc catattccag ccccagcaaa aactgcaccc ttctgctggt
     4021 gcgtctgcta agattgatga tgcggttgga tacaatattc cggttgggaa tggtttgaaa
     4081 agacttgtcc aatgcacgca atcgggtgga acgaaagcct accttatcaa ctgttccggt
     4141 aatgccatcc agctccacca gatctcctac caggaatggt ttctctgcaa acagactgat
     4201 ggaaccgaaa atgttctgca gggtttcctg tgcggcgaag gcaatggcaa tacctgcgat
     4261 acctgcaccc gctaccagac tggtaacatt gacatggaat acaaaagcca gcacacacaa
     4321 caccactgtt gtccagatga ccactttgaa aacatccttc agaaaagaca ccacctgatc
     4381 gtcactggga tcaggagtaa gctgcgcttt ttcgttcatc accgtgatga tgaaatcagc
     4441 aaagcggctg aacaaccagc cggtacttac aatgaacaga agtgtataaa gaccgtaagc
     4501 atactggtgt aacgtggcac cgataaactt aaactggaag aattcaggag catcgagcat
     4561 ctgaaacccg atataaatgg tatgcaatac cagaatgaac tccagaggtt gcagcaaaag
     4621 cttgagaaaa agctgaccgc tgtgcttgaa tctggatcgc ttcaatagct ggaacaacaa
     4681 cctgctgatg aaattggaga tgtatttatt gaaaatgatc accaccagga tcacacccag
     4741 cagccaggca tattgccgga gggtgtttcc ccatatctcg gtatccatga atgtcaggat
     4801 ggtcgccatg cggcaaaggt acaaaccatg ccctcttttt tagacaagct gccgggcttc
     4861 ctgtgtactg atggcctgta ttttcaggca tttacaggca tcttcggagc acatgatcaa
     4921 caagctgctt acccctgtgc gtactaaagg tctgctggac atcaccggca gcccgctgca
     4981 gatgggtcgc tgtatggaca tccacaccgg cgaggccccc gatacctacg agaaagacat
     5041 tgccatcatc acggtcaatg aacaacgcgg tggcgatcct gcaagaagca gcaacaaact
     5101 ggacgctatc cgaaaacatt tttacagcct gatcaatccg catgacaacc tccgtcttat
     5161 cgacgccggt gaagtgctgc tgggtgagac accggaagaa accacacaga acctgaagat
     5221 ggtggtggag ttcttcctgg cacagcgcat catcccagtc attattgggg gaagtcagga
     5281 cctgagcatc ggtcagtata aagcatacga atttctggga caggccatca acctggcggt
     5341 gatagacgaa cgcattgaca ttgatcatgc cgacgatgca ccacccagac aaacctggct
     5401 gcttgacatc ttcagccatg agccgaatta tctcttcaac tattctgccc tggcctgcca
     5461 gggtcatttt gtcaacaacg agatcttcca caccatcgat cacctttact ttgatacctg
     5521 tcggcttggc agactgcggg acaattttca gctcgccgaa cccgagctcc gcgatgccga
     5581 tatggtcagc ttcgatctct ctgccatcaa ggcttctgat gcgatgggtg tgctggacct
     5641 gagcccgaac ggtgtcaact ctgaagaggc ctgccagctc atgcgctatg cgggtatgag
     5701 cgacaaggtc agctccatgg gtatctataa ttatttgcac gagggcgaca accaggaact
     5761 gaccgcacag ctggtagccc agatgatctg gtattttgtg gatggattct acaaccgcaa
     5821 gcacgactat cccatcaccg acgagtctga tttcatgaag tacatcgtca acctcagcga
     5881 gaacgaatac gacctcactt tctggaagag caagaagtcc ggacgctggt ggctggaggt
     5941 tccttttacg gtggatactc gctacgaacg ccagcaactc gtgccctgtg cttacgccga
     6001 ctacgaagaa gccatgaaag gcacactgcc cgacaaatgg ctgcggatgt tcgagaagtt
     6061 atcggcctga ttcctccaca acataagccc cagccgggtc a
//
LOCUS       DAOMAX010000159         6029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2117_length_6029_cov_3.763140, whole genome shotgun sequence.
ACCESSION   DAOMAX010000159 DAOMAX010000000
VERSION     DAOMAX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2117_length_6029_cov_3.763140"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..440)
                     /locus_tag="PK742_08705"
     tRNA            complement(367..440)
                     /locus_tag="PK742_08705"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(404..406),aa:Lys,seq:ttt)
     gene            complement(513..1013)
                     /locus_tag="PK742_08710"
     CDS             complement(513..1013)
                     /locus_tag="PK742_08710"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HQU76708.1"
                     /translation="MNKLMYSLLMAGMISFAACGQSGEEAHDEKASGVEQNEAGGYGK
                     VVTPDNPVALTDLMDDMDKANGFQGQVTGTVVASCQNKGCWMTLDGGNGQTMMVSFKD
                     YGFFVPMDMAGKQVVIEGIAEIRTVGVDEQRHLAQDGGASEEEINAITEEKQELSFVA
                     DGVVVI"
     gene            complement(1033..1176)
                     /locus_tag="PK742_08715"
     CDS             complement(1033..1176)
                     /locus_tag="PK742_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76709.1"
                     /translation="MKKTIYLLLLGVVLGLAACSDKKDKGNDSDHDDMDQLTLVVSAD
                     QLA"
     gene            complement(1181..2431)
                     /locus_tag="PK742_08720"
     CDS             complement(1181..2431)
                     /locus_tag="PK742_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HQU76710.1"
                     /translation="MSAISTDVLVLGAGPGGAMAALFLHKEGVDCVVVDKAVFPRDKI
                     CGDALSGKVVEVLNRYDRALTDKLALRKESLPCWGVTFVAPDLEALSIPFQLSGNGQE
                     RERAPGFISRRMDFDNFLVDEVRGKDGIRLMEGCEIKSFERNADGYLFTAADGTTIQA
                     RMVIVADGAHSRFAKEVAGIEVEKEHYCAGVRAYYEGVEGIADGNYIELHFLKDLLPG
                     YFWIFPLPDGGANVGLGIRSDYASKRRINLRKELPELIKRYPQLQERFKNARLTDEIR
                     GYGLPLGSKKRPLSGDHYLLVGDAASLIDPFTGEGIGNAMLSGMLAAQQTAKAVQAGR
                     FDAAFQQPYDQAIFRRLWPELKLSYRMQRLVNYPWLFNFVVRKANRNRVLRETITVMF
                     EDIDLRDRLRKPSFYFQLLFSGKT"
     gene            complement(2461..3741)
                     /locus_tag="PK742_08725"
     CDS             complement(2461..3741)
                     /locus_tag="PK742_08725"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQU76711.1"
                     /translation="MRLALLTGLFLASFYVFGQQPVTPRTLNTWYDPAFVHASTTDSM
                     VVTSWGDAWAGSNALPSTFAYKFLFGGEVTDKIKEQATKKLDPLNTGGYDFQYGLWYS
                     SFLSFMPGYRVHVGIEQTAHRGFIFTDDLLRMGLYGNAIFADETAVFDGSGYDAKAYM
                     NLKLAVSKEAQLGKNQLFFSIGMGILEGLAGEELDISNGSIYTAPDGSYLDLDYAFTY
                     NTTGRGANKISNPRGWAVGGDLSALLIGDDNKWQAAASISDLGVLAWNHERLARYAGD
                     TTSTYEGIDVTDIVLGVQVLSSNSNSDLLLTYIDLDTLDGNFSSVTPARFDAHFSYQL
                     MEGTDLTAGVTHRLGSSFDPAVYLIGSKQFGPSEAQVMLSFGGYGGWGIGGGYGITVM
                     DHYHVDIRTPNILAFIVPGFSTVSGLQLRLSAGF"
     gene            complement(3741..5828)
                     /locus_tag="PK742_08730"
     CDS             complement(3741..5828)
                     /locus_tag="PK742_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76712.1"
                     /translation="MKKRTVWLSIGMILLVLVSCKKKETYWDINMLTPLVQGELTILD
                     MLPDSLTEVNADESLTLVYQDELLSYNFADENISIPDTLITTFVSLDSLSLDDRTIVQ
                     SITLGDVAIALGFPVGTFIILSNGSEAAIDPIADLSSGEQPIDATSFFQTATIQTGFL
                     DIKVDNGFPIPLENINLTIRNSMDGATIVDEYVAYIGPGETFTSTTPLDGMTVEGNLI
                     AEITDFSSPGSGGELVLIDTSDALVITMTAYDLELLAATAVFPAQDLIDNHNNIVYNM
                     GGPEFTQMKIKSGEVTMYVINTIQDSIHIEYSIPGATDQYGVPVYINPVVPPAPEGGS
                     VVYDQAFPLDNFTIDLRGANGTSYNTFYNEFKASIDSTGNVVSISLEDSVEVQYGLRD
                     IIPSELKGYFGQTDFSVSDTVGGLSFFESITGGNLTLTDCDVLLHIENGVGVEGDFTI
                     NQLTAVNSRTGQTVNLYAPGIVNAPISVNRATNNPFIPGQNILELTPDNSNILDVLGI
                     LPDKLIYDINMQVNPNGNEFNYQDFFIEASRINFFIDMEIPMEFIASDLVLEKQVETK
                     FNELSNREDIKSGTFTLHVDNGFPFSAGIAMTFYDRNQEELTTVNFSNPIAAGTLDGE
                     CKVSSPTYSELSAIIDENTMMDILRSGTALVTATFNTESAEGCSDAVKIYSDYSIDFQ
                     LTADFTYTLNIGN"
BASE COUNT         1396 a         1553 c         1544 g         1536 t
ORIGIN      
        1 actgtatgaa tgtagtccat atatgctcaa attgacatac tatgaaaata aggaagtaaa
       61 gcatcttatc tgaaacttag attggctttg ccggccccct ttcagttggc gataggcagt
      121 ttggagttgg ccgtctgctc aaacctctgg gttctgttga cgaggattac aatgtgattg
      181 gtcttattga tcgctgggat tggcttcgcc ggcccctggc ttatccagag tcagaatgat
      241 caaactgcgc ctgctatcgc agccgggttt tcttttggct cacatccgcc cgaacctctg
      301 ggttcggctg ctgttcacca aaagaaaact cccgctcttt tggagcggga gttgatcatt
      361 tatctgtgat tccgctggga ctcgaaccca ggaccccaac attaaaagtg ttatgctcta
      421 gccagctgag ctacggaatc gtgccacctt tttttcaaag gtggggcaaa gataatcgtt
      481 tgagctattc tgcccaaatg ctgtggataa aatcagatga ccaccacacc gtcggccacg
      541 aagctgagct cctgcttctc ttcggtaatg gcattgatct cttcttcgga ggcgcctccg
      601 tcctgggcca ggtggcgctg ctcatccacg cctaccgtgc ggatctcagc tattccctca
      661 atgaccactt gctttccggc catgtccatc ggcacgaaaa agccatagtc tttgaagctg
      721 accatcatgg tttgtccgtt gcccccgtcc agggtcatcc agcaaccttt gttctggcaa
      781 ctggctacta cggtgcctgt cacctgcccc tggaagccat tggccttgtc catatcgtcc
      841 atcagatctg tcagggctac aggattatca ggtgttacca ccttaccgta tccgccggcc
      901 tcattctgtt ctacgccgga agctttttca tcatgtgcct cttcgcccga ctgtccgcag
      961 gcggcaaaag agatcatgcc tgccatcagc aggctgtaca tcaacttgtt catatgcata
     1021 ttggttttta gatcaggcca gctgatcagc agataccacc agggtgagct ggtccatatc
     1081 gtcgtgatca ctgtcattgc ctttgtcttt cttgtcgctg caggcagcca gtccgagcac
     1141 tacgcccagc agcaagagat aaatggtttt tttcatgtcg ttatgtttta cccgaaaata
     1201 acaattgaaa ataaaatgaa ggcttccgga ggcggtcacg caggtcaata tcttcaaaca
     1261 tgaccgtgat ggtctcccgc agtacccggt tccggttcgc ctttcgtacc acaaaattaa
     1321 acaaccaggg gtaattgacc aggcgctgca tgcggtagct gagcttcagt tcgggccaca
     1381 gcctgcggaa aatggcctgg tcataaggct gctggaaagc ggcatcaaaa cgtccggcct
     1441 gcacagcttt ggctgtctgt tgtgcagcca gcatcccgct cagcatagca ttgccaatgc
     1501 cttcgccggt aaatggatct atcaggctgg cagcatcgcc cacgaggaga taatgatcgc
     1561 cggaaagggg gcgttttttg gaccccagtg gcagcccata gcccctgatc tcgtcggtga
     1621 gccgggcatt tttgaatctc tcctgcagtt gcggatagcg ttttatcaac tccggtaact
     1681 ctttgcgcag gttgatccgc cttttggaag cgtagtctga acggatgccc agccccacgt
     1741 tggcaccacc atccggcaat gggaatatcc agaaatatcc tggcaggaga tccttgagaa
     1801 aatgcagttc tatatagttg ccgtcggcta ttccttccac cccttcataa taggcacgca
     1861 caccggcgca atagtgttcc ttttccactt cgatgccggc cacttctttc gcaaagcgac
     1921 tgtgggcacc atcggccaca atgaccatcc tcgcctgtat ggtagtacca tcggcagcag
     1981 tgaagaggta accatcggca ttccgctcaa aacttttgat ctcacatcct tccatcagcc
     2041 ggatgccatc ctttccccgc acctcatcca ccaggaagtt gtcaaagtcc atccgcctgc
     2101 tgatgaatcc cggagctctt tccctttcct gtccgtttcc gcttagttga aaaggaatac
     2161 tcagggcttc cagatcgggt gccacgaagg tcacacccca gcagggcaga ctttcctttc
     2221 gcaatgcaag tttatcggta agcgcacggt catagcggtt gagtacttcc accaccttgc
     2281 cgctgagggc atctccgcag atcttgtcgc ggggaaatac cgccttgtcc accaccacac
     2341 aatccacgcc ttccttgtgc aggaacagcg ctgccatggc ccctccggga ccggctccca
     2401 ataccagaac atcagttgaa atggccgaca tgagcggcaa aaatacagga atcggggcac
     2461 ttaaaatccg gcactcaagc ggagttgcaa accgctcacg gtgctgaagc ccggaacgat
     2521 gaaagcgagg atgttcgggg tgcggatgtc cacgtggtaa tgatccatca cggtgatgcc
     2581 atagccaccg ccgatacccc aaccgccata tccgccaaag ctcagcatga cctgggcttc
     2641 tgatgggccg aattgtttgc tgccaatgag gtacacggcc ggatcaaagc tagacccgag
     2701 gcgatgggtg acaccggcag tgaggtcagt tccttccatc agctggtagg agaagtgcgc
     2761 atcgaaacgg gcaggagtga cgctgctgaa attaccatcc agggtatcga ggtcaatata
     2821 ggtgagcagc agatcgctgt tgctgttgct gctcaggacc tgcactccca gcacgatatc
     2881 ggtcacgtct ataccttcat aggtggaggt ggtatcgccg gcataacgag ccagtcgttc
     2941 gtgattccat gccagaacgc ccaggtcaga tatgctggct gcggcctgcc acttgttgtc
     3001 atcgcctata agcaaagcag agagatcgcc acccacagcc cagcctctgg gattgctgat
     3061 cttgtttgct ccacggcctg tggtattata agtgaaggca taatcaaggt ccaggtagga
     3121 tccatcgggt gcagtgtaga tgctgccatt gctgatgtcc agttcttctc cagccagtcc
     3181 ttccagaata cccatgccaa tggaaaagaa gagctgattt ttccccaatt gtgcttcctt
     3241 gcttacggcc agtttgaggt tcatgtaggc ttttgcatca taaccgctgc catcaaacac
     3301 cgcagtttca tcggcaaaga tggcgttgcc atagagaccc atacgcagca gatcgtctgt
     3361 gaagatgaat cctctgtgtg cggtttgttc tatgcctaca tgcacccgat aaccgggcat
     3421 gaaggagagg aaagaagaat accagagacc atattgaaaa tcatagccgc ccgtattcag
     3481 cggatccagt tttttagtgg cctgttcttt gatcttgtcg gtcacttcac ctccgaataa
     3541 aaatttatag gcgaaggtgg atggcaaagc attgctgccg gcccaggcat ctccccatga
     3601 agttaccacc atgctgtcgg tggtactggc atgtacaaat gccggatcat accaggtgtt
     3661 cagcgtgcgg ggagttaccg gctgctgacc gaatacataa aaagaggcaa ggaacaaccc
     3721 cgtcaggaga gccagtcgca tcagttgcca atatttaggg tgtaggtaaa gtcggcggtc
     3781 agttgaaagt cgatggaata atcgctgtag atcttcacag catcgctgca gccttcggcc
     3841 gattccgtat tgaaggtagc agttaccagg gcggtgcctg aacgaaggat gtccatcatg
     3901 gtgttctcat caatgatggc gctgagttca gaataggtag ggctgcttac cttacattcg
     3961 ccatccagtg taccggcagc aataggatta ctgaagttaa ccgtggtcag ctcttcctgg
     4021 ttgcgatcgt agaaggtcat ggcgatgccg gcagagaagg ggaagccgtt gtccacatgc
     4081 aaggtgaaag tgccgctttt gatatcctcc cgattgctca gttcattgaa ttttgtttcc
     4141 acttgttttt ccagtaccag atcagatgcg atgaactcca tgggtatctc catgtcgatg
     4201 aagaaattga tccggctcgc ttcgataaag aaatcctggt agttgaattc gttgccgttt
     4261 ggatttacct gcatgttgat gtcatagatc agcttgtcag gcaggatgcc cagcacatcc
     4321 aggatgttgg aattatccgg cgtcagttcc aggatattct ggccgggaat aaaaggatta
     4381 ttggtagccc ggttcacaga gatgggagca ttgacaatac ccggagcgta gaggttgacg
     4441 gtttgtccgg tcctgctgtt cactgctgta agctggttga tggtgaaatc accttccaca
     4501 ccaacaccgt tctcgatatg cagcaataca tcgcaatctg tcagggtgag gtttccaccg
     4561 gtgatgcttt caaagaaaga gaggccaccc acagtgtcgc tgacactgaa atctgtctgg
     4621 ccgaaataac ctttaagttc actgggaatg atatctctga ggccgtactg cacttctact
     4681 gaatcttcca gcgatatgct caccacattt ccggtgctgt ctatactggc cttgaattca
     4741 ttatagaaag tattgtagga agtaccattg gcaccacgca ggtcgatggt gaaattatcg
     4801 agcgggaatg cctgatcata caccacgctg ccaccttcgg gagccggggg tactacagga
     4861 ttgatataca ccggaacacc atattgatca gtagcacccg gaatggagta ttcaatgtgg
     4921 atgctgtctt gtatcgtgtt gatgacatac atggtcactt cgcctgattt gatcttcatc
     4981 tgtgtgaatt ccggaccacc catattatag acgatgttgt tgtggttgtc tatcaggtcc
     5041 tgggcaggaa atacggcagt tgctgcaagt agttcgagat cgtatgctgt catggtgatg
     5101 accagtgcat cggaagtgtc gatgagtacc agttcacctc cgcttcccgg agaactgaaa
     5161 tctgtgatct ctgcgatgag gtttccttcc acggtcatgc cgtccagcgg ggtagtagat
     5221 gtgaaagttt cacccggacc tatataggcc acgtattcat ctacgatggt agcaccatcc
     5281 atactgttgc gaatggtcag attgatattt tcaagcggga tggggaagcc attgtccact
     5341 ttgatatcca gaaatccggt ctggatggta gcggtctgga agaaggaagt ggcatctata
     5401 ggttgttctc ccgacgacag atctgcgatg gggtcgatgg ctgcttcgga tccgttgctg
     5461 aggatgatga aagttcctac cggaaagccg agggcgatgg ccacatctcc cagtgtgatg
     5521 ctttgtacga tggttcgatc gtccagcgaa aggctgtcca gtgacacgaa ggtggtgatc
     5581 agtgtatcgg ggatggagat gttctcgtcg gcgaaattgt agctcagcag ttcgtcctga
     5641 tatacaaggg tgaggctctc atctgcattg acctccgtaa gactgtctgg caacatatcc
     5701 agtatggtca gttcaccctg taccagaggg gtcagcatgt tgatatccca gtatgtttcc
     5761 tttttcttgc aggaaaccag caccagcagg atcataccga ttgacagcca gacggttctc
     5821 tttttcatat acccgtaacg aagataggga gttaggcgat acagggggca cagacaggcg
     5881 gtggattgtg taaattttgt gacaaagcag ttggtcccga tagctatcgg gatgggttcc
     5941 accctttcac cttttcattc cggctttcgc ctttcgactt ccgccttaga acctcctccc
     6001 ggtatggcat caatgagcct ctgtgtgta
//
LOCUS       DAOMAX010000160         6024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2120_length_6024_cov_4.361367, whole genome shotgun sequence.
ACCESSION   DAOMAX010000160 DAOMAX010000000
VERSION     DAOMAX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_6024_cov_4.361367"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK742_08735"
     CDS             complement(<1..210)
                     /locus_tag="PK742_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76713.1"
                     /translation="MKKQLLLLLLLATGLGLAFAQPEGEDLESIRKHEKTLQVLGDSV
                     VQSFDWDMREQACIQMVKTLVKALKI"
     gene            complement(291..1469)
                     /locus_tag="PK742_08740"
     CDS             complement(291..1469)
                     /locus_tag="PK742_08740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQU76714.1"
                     /translation="MKEVVIVSVARTPIGSFGGGLASLTAPKLGAVVIKAALERAGIS
                     GDQVNEIFMGNVLSAGIGQAPVTQAMLAAGIPDTTPSTTINKVCASGMKAIMIGAQSI
                     MLGTNDIVVAGGMESMSNVPYYLTKERWGAKLGHQQAIDGIIHDGLWDPYGNKHMGSC
                     GETCAREYNFSREDQDNYAADSYRRAAEAWEKGWFNNEVVPVSIPQRKADPIIVDKDE
                     DYTKVKFDKLSSLPPAFEKDGTVTAANASNLNDGAAAVVLMSRDKAEELGIKPLARIA
                     GMADAQQAPEWFTTTPAKAMPKAIAQAGLQISDVDYFEINEAFSVVALANMKDLNITH
                     EQTNVYGGAVSIGHPIGCSGARILITLLSVLQQKEGRYGCAGICNGGGGASAVVIEKL
                     "
     gene            1577..4282
                     /gene="mutS"
                     /locus_tag="PK742_08745"
     CDS             1577..4282
                     /gene="mutS"
                     /locus_tag="PK742_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQU76715.1"
                     /translation="MQTEKQHNQDIPDLLRQEMTEQDNGCIPLALAIFAGMKGGKETP
                     LMAQYNSIKAKYPDAILLFRVGDFYETFSSDAILTSRILGIVLTKRANGSASHVELAG
                     FPHHALDTYLPKLVRSGHRVAICDQLEDPKLTKKIVKRGVTELVTPGLATGDKLLDSG
                     NNNYLAAWHAGKEAMGVSFLDISTGEFLVAEGGRDAMEKLLFTFQPAEVLVEKAQQKL
                     FKQHFGERFYLFALEDWAFTEDHAREKLLRHFDTTTLKGFGIDGMTEAVRAAGASVHY
                     LGETEHSNLEHIRSISRLAEEEHVWLDRFTVRNLELLHPSMEGGSSLFDVLDHTITAM
                     GTRLLKRWILMPLKDLSRIKNRLDLVEELVRERDLSAGIATQLRHCGDLERMASKLSM
                     RKINPREVVQLKRSLDAVAQLALDLQESAAEPVRQLAGRLHPCADIRRRMEDTLEEEA
                     PVQLHKGNVIKPGISAELDQLRDIGQSGKEYLVRIQQREAAETGISSLKIGFNNVFGY
                     YLEVTNRYKDQVPDTWIRKQTLTNAERYITPELKEYEEKILGAEDRIQSIEQQLYEEL
                     LLAVGEYLPQILSNAAVVATVDCLLNFAEVAILHNYRKPLVNDGYKLWLSACRHPVIE
                     QHLPVGEAFVPNDLLLDPESQQIIILTGPNMAGKSALLRQAALNVCMAQMGCFIPAEA
                     AEIGLVDKIFTRVGASDNISSGESTFMVEMTETASIMNNLSDRSLVILDEIGRGTSTY
                     DGISIAWSLCEYLHQREQSAKVLFATHYHELNELENRFDRIVNYHIATRQSGNKVIFL
                     RKLTKGGSQHSFGIHVARMAGMPKEIIGRADGILKELEQKHIGENLKKKLGTIPAQPM
                     QLNIFGMEDPVWNDIRKELDELDINGLTPIEALLLMQQWKNKLDS"
     gene            4353..>6024
                     /locus_tag="PK742_08750"
     CDS             4353..>6024
                     /locus_tag="PK742_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76716.1"
                     /translation="MKKLYLKGVVAMSLSLFTASLTQAQLVGGDAYLQGTYVEAGLNQ
                     CGAYASSVSPPAGYEVTGLTGLNFIADSDMDGWGAGTPEYCGDYAIPGSPVEGWGIQI
                     AGTSYHNTDRTCATFNISGSISSYTAEGDSVEALWEGSVAGVDISQRSVLHADDLYVL
                     TWITLTNTTAEPISDIYYKRNIDPDNEQIWSGSYVTINSVTSSPFSGDLDAVVTAEGA
                     LYGCYLALAARNPQARPSYGGFSTVGGAVSDAYNGITPYNLSGSVTVDGAVQMSFYIP
                     ALGAGESTTFAMAHVFSADALEEALDATYVGGYVDCAGTPEVGGAFATAESTCASDVF
                     TLSVDVAAAVGITYQWQSSADGITYADIAGATGPSYSTTQTDATYYQCVVSCTASGEA
                     TTSDAVFVDNVCPGCTDEAALNYNPEANSDDGSCFYGYTILDCDYGSSLISVPEDATE
                     LCLSDDEVSAAISMGFTFDFYGTPYTNAYIGSNGYMNFSGTTLSACCAGQLLPNGAYP
                     ASIFFGQNDWNPAFCGAGTISYWTQGAPGSQIFVVDFNAVPHYGGDLTN"
BASE COUNT         1487 a         1510 c         1522 g         1505 t
ORIGIN      
        1 gatcttcagg gctttcacca gtgtcttgac catctggata caggcctgct cccgcatatc
       61 ccagtcaaaa gactgcacca ctgaatcgcc caatacctgc agggtctttt cgtgttttct
      121 gatggactcc aggtcttccc cttccggttg tgcaaaagca agccccaaac cggtagccaa
      181 cagcagcagt aacaagagtt gtttcttcat ggcagaaaaa atcctgatac ccgttgcggt
      241 gcaaaaagta tcaggatata taatgagttg tttcctcaat tattgcagga tcacagtttt
      301 tcgatcacca cagcagaagc gccgccacca ccattgcaga tacctgcaca accgtatctg
      361 ccttcctttt gctgcagtac agacaagagg gtgatcagga tgcgggcacc cgaacaaccg
      421 attggatgac cgatagaaac agcaccacca tacacgttgg tctgttcatg agtaatgttc
      481 aggtctttca tattggccag agccaccacg ctgaacgctt cgttgatctc gaaatagtcc
      541 acatcactta tctgcaggcc tgcctgggca atggcttttg gcatggcttt cgccggtgtg
      601 gtagtgaacc attcaggagc ctgctgggca tctgccattc cggcaatgcg ggcaaggggt
      661 ttgatgccca gttcttcggc cttatcgcga ctcatcagca ccactgctgc agcgccatca
      721 ttcaggttag atgcattggc cgcagtcacg gtaccatctt tttcgaaagc tggtggcagg
      781 gatgatagct tgtcgaattt gactttggtg tagtcttcgt ctttatctac gatgatggga
      841 tcagctttcc gctgggggat actgaccggt accacttcat tgttgaacca gcctttttcc
      901 caggcttcgg ctgcccggcg ataactgtcg gcagcatagt tgtcctgatc ttcccggctg
      961 aaattgtatt cacgggcaca ggtctctccg caggaaccca tatgcttgtt gccataagga
     1021 tcccaaagtc cgtcgtgtat gatgccgtct atagcctgct ggtgacctag tttggcgccc
     1081 cagcgttctt ttgtgaggta atagggtaca ttgctcatgc tctccattcc tccagccaca
     1141 acgatatcat tggtacccag catgatggac tgagcaccta tcatgatggc cttcattcct
     1201 gaggcacata ccttgttaat ggtggtagag ggagtggtat caggtattcc ggctgcaagc
     1261 atggcctgcg ttacaggcgc ctggccaata cctgcactca acacattacc catgaagatc
     1321 tcattgacct gatcaccgct gataccggcc ctttccagag ctgctttgat gaccactgca
     1381 cccagttttg gcgctgtcag agaagccaga ccaccgccaa aacttccaat gggggtgcgg
     1441 gctactgata cgatcactac ttctttcatc attatacaat tagattattg attttcttat
     1501 ttcggtgcaa aggtaaggtg tttattgctg gagcgctcct gttcatggct gtttctgtca
     1561 acacctgctg gtgcaaatgc agacagaaaa gcagcacaat caggatattc ctgacttgct
     1621 ccggcaggaa atgacggaac aggataatgg gtgcatccct cttgctttgg ctatatttgc
     1681 cggcatgaag ggcggcaagg aaacaccttt gatggcacag tacaacagca tcaaagcaaa
     1741 atatcccgat gctatcctcc tgtttcgtgt aggcgatttc tatgagacct tttccagtga
     1801 tgccattctt acctcccgca ttcttgggat cgttctgacc aaacgtgcca atggttcagc
     1861 ttcacatgta gaactggccg gctttccgca ccatgccctg gacacctatc ttcccaagct
     1921 ggtgagaagt ggccatcggg tagccatctg cgatcagctg gaagacccca aactcactaa
     1981 aaagatcgta aaacgggggg tcaccgaact ggtaactccc ggccttgcca caggcgataa
     2041 actcctggac tccggcaaca acaactacct ggctgcctgg catgccggga aggaagccat
     2101 gggtgtaagt tttctggata tcagtacagg cgagtttctg gttgcagaag gcggacgtga
     2161 tgcgatggag aaactcctgt tcacctttca gccggcggaa gtgctggtgg aaaaagctca
     2221 acaaaaatta ttcaagcaac attttggcga acgtttctat ttgtttgccc tggaagactg
     2281 ggcctttacc gaagaccatg ccagagagaa actacttcgg cattttgata ccactaccct
     2341 gaagggcttt ggtattgacg gcatgacaga agccgtgcgg gcagcaggtg cttctgtaca
     2401 ttatctgggt gaaacagaac acagcaacct cgagcatatc cggtccattt cgcggctggc
     2461 agaagaagag catgtatggc ttgaccggtt caccgttcgc aacctggaac tcctgcatcc
     2521 atccatggaa ggcggcagca gtctttttga tgtattggac cataccatca ccgccatggg
     2581 cacccggttg ctcaagcgct ggatcctgat gcctctgaaa gatctgagca ggatcaaaaa
     2641 ccgcctggat ctggtggaag aactggtcag ggaaagagac ctctctgcag gcattgccac
     2701 gcagctgaga cattgcggcg atctggagag aatggccagc aagctgagta tgcgaaagat
     2761 caatccccgg gaagtggtgc aactgaaacg ttctctggat gcagtggcac agctggcact
     2821 tgacctgcag gagtcagctg cagaacctgt cagacaactg gccggcaggc tgcatccctg
     2881 tgccgatatt cgccggcgca tggaagatac cctggaagaa gaagctccgg tacaattgca
     2941 taagggcaac gtcatcaagc cgggcatttc ggctgagctg gatcagttgc gtgatatcgg
     3001 gcaaagcggg aaagaatatc tggtgcgcat tcaacaaagg gaagcagcag agactggcat
     3061 cagcagtctg aagatcggct tcaataatgt gttcgggtat tacctggaag taaccaaccg
     3121 ctataaggat caggtacctg atacctggat tcgcaagcag accctgacca atgccgagcg
     3181 gtatatcaca ccagaattaa aagaatacga agaaaaaata ctgggtgctg aagacagaat
     3241 acagtccatc gaacagcaat tgtatgaaga gcttctgctg gcagtaggtg aatacctgcc
     3301 gcagatcctg tctaatgccg cagtagtggc cacggttgat tgccttctta acttcgctga
     3361 ggtagcaata ctccataatt accgaaagcc tctggtgaat gatgggtaca aattgtggtt
     3421 gagtgcctgt cgtcatccgg tcatagaaca gcatctgccg gtaggcgaag cctttgttcc
     3481 aaatgacctg ctgctggacc ctgaatctca gcagatcatc attctgaccg ggccaaatat
     3541 ggccggtaaa tctgcgttgc ttcgacaggc ggctctcaat gtttgtatgg cgcagatggg
     3601 atgcttcatt cctgccgaag ctgctgagat cggtctggtt gacaagatct ttaccagagt
     3661 aggtgcttca gataatattt cttccggcga gtccaccttt atggtggaga tgacagagac
     3721 tgccagcatc atgaacaacc tgagtgaccg gagtctggtc attctggatg agataggaag
     3781 aggaacaagt acctatgatg gcatttccat cgcctggagt ctgtgtgagt atctgcatca
     3841 gcgggaacag tcggccaagg tattgttcgc cacccactat catgagctga acgagctgga
     3901 gaatcgcttt gaccgcatcg tgaactatca catcgccacc aggcaatcgg gtaataaggt
     3961 catcttcctc cgaaagctta ccaaaggcgg cagccagcac agctttggta ttcatgtagc
     4021 caggatggca ggtatgccca aagagatcat aggtcgtgcc gatggcatcc tgaaagaact
     4081 ggagcagaaa cacattggag agaacctgaa aaaaaagctg ggtactattc ctgcacaacc
     4141 catgcagctc aacatattcg ggatggaaga tccggtctgg aacgacatcc gtaaagaact
     4201 ggacgagctc gatatcaacg gcctcactcc tattgaggcg ttactgctta tgcaacaatg
     4261 gaaaaataag ctggacagct gacacttttt tgacattccg gaaaaataag cggtacattt
     4321 aaggtactca tcttaaacct aaatagaact gtatgaaaaa actgtacctg aagggtgtgg
     4381 tggctatgtc actctctctt tttacagctt ccttaacaca ggcccagcta gtaggcggcg
     4441 acgcatacct gcagggtact tatgtggaag ctggtttgaa ccaatgcggc gcttacgctt
     4501 ccagtgtatc gcctcctgcc ggttatgaag tgaccggtct gacaggactg aacttcattg
     4561 ccgattcaga tatggatggc tggggagccg gtactccaga gtattgcggc gactatgcca
     4621 tacccggatc tccggtggaa ggctggggaa ttcagatcgc aggaacttct taccacaaca
     4681 ccgacagaac ctgtgcaact ttcaatattt caggttccat ttcttcttac acagccgaag
     4741 gcgattcagt agaagcttta tgggaaggat ctgtggccgg agtagatatt tcccagcgca
     4801 gcgtactgca tgcagatgac ctgtacgtgc ttacctggat caccctgacc aacacgaccg
     4861 ctgagccgat ttccgatatt tattacaagc ggaatattga tcccgataac gaacaaatct
     4921 ggtctggatc ttatgtgacc attaattcag taacatcatc gccctttagc ggtgatctgg
     4981 atgcagtggt aacggccgaa ggagctttgt atggttgtta tctggcactg gcagcccgca
     5041 acccgcaggc gagaccttcc tatggaggct tcagtaccgt aggaggtgct gtatctgatg
     5101 cctataatgg tattacacct tataaccttt caggttcagt cactgttgac ggagctgtgc
     5161 agatgagctt ctacataccc gctcttggag caggtgaaag caccacattt gccatggctc
     5221 atgtattcag tgcagatgcc ctggaagaag ccctggatgc tacctatgtg ggtggttatg
     5281 tagattgtgc aggtacccct gaagtaggcg gtgcattcgc aactgccgaa agcacctgtg
     5341 catcagatgt gttcacttta tcagtagatg tagctgcagc ggtaggtatc acttaccagt
     5401 ggcagagttc tgccgacggt atcacctatg ctgatattgc cggagctacc ggcccgtctt
     5461 acagcactac ccagaccgat gctacctatt atcagtgtgt ggtttcctgt accgcttctg
     5521 gtgaggctac cacttcagat gccgtatttg ttgacaacgt ttgtccaggt tgtactgacg
     5581 aagctgctct caactacaac ccggaagcca actcagatga cggatcctgc ttctacggat
     5641 ataccatcct ggattgtgat tacggctcca gcctgatctc cgttccggaa gatgctactg
     5701 aattgtgtct ttcagatgat gaagtttcag cagctatcag catgggattc acctttgact
     5761 tttatggcac accatatacc aacgcctata tcggctccaa cggttatatg aacttcagcg
     5821 gcacaactct gagtgcttgt tgcgctggtc aattgctgcc caatggtgca tatcctgcca
     5881 gtatcttctt tggccagaat gactggaacc cggcattttg tggtgcaggt accatttctt
     5941 actggactca gggtgccccc ggaagccaga tattcgtagt tgacttcaat gctgttcctc
     6001 attacggcgg tgacctgacc aata
//
LOCUS       DAOMAX010000161         5965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2161_length_5965_cov_4.278173, whole genome shotgun sequence.
ACCESSION   DAOMAX010000161 DAOMAX010000000
VERSION     DAOMAX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2161_length_5965_cov_4.278173"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK742_08755"
     CDS             complement(<1..523)
                     /locus_tag="PK742_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQU76717.1"
                     /translation="MAQIIYLIRHSKWFWMPVLVFLLLGLCWIWQVDNTAISLWINGR
                     HSIAGDVFWRAMTWMGDGITMSILIFLLLFIRFRTAFLAAAALLVSSLAAQWLKHFFA
                     HDRPSLVLSGMDLHLVPGVQLYAHFSFPSGHTTAAFCIYGVLAVLSGRPVLQWLFFLI
                     AALVGISRIYLLQH"
     gene            complement(511..1950)
                     /locus_tag="PK742_08760"
     CDS             complement(511..1950)
                     /locus_tag="PK742_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQU76718.1"
                     /translation="MLKIIDKYILRSFIGPFVVTFFIALLILLIQFLWKYIEDLVGKG
                     LEWWVIGKLLLYASANMIPLALPLATLLSSIMLYGNLGERFELAAMKSAGISMFRFMR
                     STIVAGLIITAGAFLFSNYVLPTASLKFWTTLIDITRQKPALNIKPNEYYNEIENYSI
                     LIGGKAEDNISISDITIYEEGQNTNQGNVLIAESGQMLPSADKRNLTLKLYHGKQYEE
                     LKPEVRNKPSYEHVRMEFGEWEKALDLSGFQMQESSEDLYRDHYKMMNVSQLSKAIDT
                     LQMQADEKNHSLKPVIGAYYLFNRDTLILADMSLLHLSEEQQKAGSVAYLAPPEDRQV
                     IVQRALSTVRNISNFNRQVEESLHFTSNKIAEHRIEWHRKFTLSIACLVFLFIGAPLG
                     AIIRKGGFGLPVIFSILIFIIYYVLTITGEKRADQGLVTPLFGMWIGILILFPFSILL
                     TQKAKNDSPVFNRDWYLLQWDRISRLWRR"
     gene            1972..2511
                     /locus_tag="PK742_08765"
     CDS             1972..2511
                     /locus_tag="PK742_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76719.1"
                     /translation="MVFTSEEIALLNDRQIFLQKRSLLEKIGAVFARTEQLLKDTAIG
                     SQFAFPANCHLASGKTSRGENLLGYPWMVHDYPRLINRDEIFLFRTQCWFGHGFSCSW
                     ILTGRHAGLLAGKPLKANWTMATDEHIWDQSIQIIADQFPETLCPAVRLTMPLGNDPK
                     GLEERTSIIFRETLDLLGA"
     gene            complement(2512..2895)
                     /locus_tag="PK742_08770"
     CDS             complement(2512..2895)
                     /locus_tag="PK742_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HQU76720.1"
                     /translation="MGKTVKYELEYIVRSSPSVLHNFLITPTGLSQWFADNVDNEGNT
                     YFFTWNGVEEEAELLTTDDDDSIRFRWDYYDDDEFFEFRIEQGEITGDTILYITDFAE
                     PNEVDSQKALWDSQIKNLIKRIGAA"
     gene            3123..4574
                     /locus_tag="PK742_08775"
     CDS             3123..4574
                     /locus_tag="PK742_08775"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQU76721.1"
                     /translation="MSTNTYEIKMNEWNHKEMDATELGMITVRLWFDKAIELIIFRRS
                     LVDARGSEILNHHLYAQRFLNKPLTIDVTLSIAREMEKLDLAPARIDLGRLGSEWLEE
                     KDNYDSISSFLQDKLKDLIGQDKINLQPRDVVLYGFGRIGRIAARILINETGKGSQLR
                     LRAIVTRSNSDEDILKRASLLRKDSVHGKFHGTIVEDLVNKALIINGVTVHMIAANKP
                     EDIDYTEYGINDALIIDNTGVARDREGLGKHMLSKGASMVLLTAPGKGDIPNIVFGVN
                     HEEYNAENEKIFSAASCTTNAVVPVIHVMENTFGIDRGHIETVHSYTNDQNLLDNYHK
                     KYRRGRSAPLNMVITETGAAKAVTKVIPSMDGKLTGNAIRVPTPNVSMAILNLTLKNG
                     VTVESVNEALKEASIHGNLIEQIDYSLSQEMVSTDCVGNGHAVVVDAPATIVSADGKS
                     VVLYCWYDNEFGYTKQVVRLSKYLSGVIRLRYY"
     gene            4598..5935
                     /gene="ffh"
                     /locus_tag="PK742_08780"
     CDS             4598..5935
                     /gene="ffh"
                     /locus_tag="PK742_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQU76722.1"
                     /translation="MFESLSNRLESAFQSLKGQGRITEINIATTVKEIRRALVDADVN
                     YKIAKEFTDTVKEKALGGDVLKSVSPGQLMVKIVEDELTTLMGGTSTDINLKGSPAVI
                     LIAGLQGSGKTTFSAKLARYIKSKSGRNPMLVACDVYRPAAINQLQVLGEQIGVQVYA
                     DTENKNPVDIAKKAISFAKVNNHNVVIIDTAGRLAVDEEMMDEITRIKQAVQPAETLF
                     VVDSMTGQDAVNTAKAFNDRLDFDGVVLTKLDGDTRGGAALTIKYVVNKPIKFISQGE
                     KTDTLDVFHPDRMARRILGMGDIVSLVEKAQEQYDAEQAAELQRKIRKNKFDFDDFLS
                     QIGQIKKMGNIKDLMGMIPGVGKQLKDLDVDEKQFKRLEAMIQSMTPAERQDPDLLNG
                     ARRKRIADGSGTSIQDVNQFIKQFEEMRKMMKMMNKMGGGAKAAKALGMMGRR"
BASE COUNT         1649 a         1407 c         1472 g         1437 t
ORIGIN      
        1 aatgttgcag caggtagatc ctggaaattc ccaccagtgc agcgatcaga aagaacaacc
       61 attgcaagac cggcctgcca ctcagcacag ccagtactcc gtagatgcaa aaagcagctg
      121 tggtatgccc tgaaggaaaa ctgaaatgag catatagctg tactcccggc accaggtgca
      181 gatccattcc tgacaatacc agtgaaggtc ggtcatgagc aaaaaaatgc ttgagccact
      241 gagccgccag gctacttaca agcaatgcag cagcagcaag aaaagcggtt cggaaccgga
      301 tgaacaacaa cagaaagata agtatggaca tggtgatgcc atcgcccatc caggtcatag
      361 ccctccagaa cacatcgccg gcaatgctgt gcctgccatt tatccataag gaaatagcgg
      421 tattatcaac ctgccagatc cagcacagac ccagcaacag aaataccaga acaggcatcc
      481 agaaccactt gctgtgtctt atgagatata ttatctgcgc cataatctgc tgatccggtc
      541 ccactgtaac agataccagt cacggttaaa taccggtgag tcgttcttgg ccttctgcgt
      601 caggagtatg ctgaatggaa aaagtatcag tatgcctatc cacatgccga acaagggtgt
      661 taccagaccc tgatctgccc gcttctcgcc ggtaatggtc agcacataat atataatgaa
      721 tatcaatata ctaaagatca ccggcaaacc gaacccaccc ttcctgatga tggctccaag
      781 tggggcacca atgaacagaa agaccaggca ggcaatggat aaagtgaact ttctgtgcca
      841 ttcgatcctg tgttctgcaa tcttattact tgtgaaatgc aatgattctt caacctgacg
      901 gttgaaattg gaaatattgc gcaccgtgct taatgcccgt tgcacgatga cctgtctgtc
      961 ttccggtggt gccaggtaag ctacagaacc tgccttttgc tgctcttcac tcaggtgcag
     1021 cagtgacata tctgccagta tcaaagtatc tctgttgaac aaatagtagg ctccaatgac
     1081 aggcttcaga gaatggttct tttcatcagc ctgcatctgc agggtatcaa ttgctttaga
     1141 aagctgagac acattcatca ttttgtaatg atcacggtac agatcttcac tcgattcctg
     1201 catttgaaaa ccacttaggt caagggcttt ctcccactcg ccaaactcca tcctgacatg
     1261 ctcatagctt ggcttgttgc gcacttcagg ttttagttct tcatattgtt tgccgtgata
     1321 cagcttaagt gtaaggttac gcttgtctgc acttggaagc atctgtccgc tttcggcaat
     1381 aagtacattt ccctgattgg tgttctgccc ttcctcataa atggtgatat ctgaaatact
     1441 gatattgtct tctgctttcc ctccaatcag gatggaataa ttctcgatct cattgtagta
     1501 ttcattaggc ttgatattca aagccggttt ttgccgggtg atgtctataa gagtggtcca
     1561 gaatttcagc gaggcggttg gcaggacata gttgctgaac aaaaaagctc cggcagttat
     1621 gatcagacct gccacgatgg tggatcgcat gaagcggaac atggaaatac ctgcagattt
     1681 catggctgcc agttcaaaac gttcacccag attgccatac aacatgatgg aagacagcag
     1741 ggtggccagt ggaagagcca ggggaatcat attggctgat gcatacaaca acagctttcc
     1801 tatgacccac cattcaagtc ctttaccgac cagatcttct atatatttcc acaggaactg
     1861 gatcagcagg atgagcagtg caataaaaaa cgtcactaca aaaggcccga tgaacgaacg
     1921 aaggatatat ttgtctatga tctttaacat gaggtccgga ccaacttgct gatggtattc
     1981 acttctgaag aaatagccct gctgaatgac cggcagatct ttctccaaaa acgcagttta
     2041 ctggagaaaa ttggtgcggt tttcgcccgg accgaacagt tgctcaaaga tacagcaatc
     2101 ggcagccaat ttgcatttcc ggcaaactgc cacctggcca gcggaaagac cagcagaggt
     2161 gagaacctgc tcgggtatcc gtggatggta catgattatc cccgcctgat caaccgtgat
     2221 gagatctttc ttttccgcac ccagtgctgg ttcgggcatg gtttttcctg ttcctggata
     2281 ctgacaggca ggcatgcagg attgttggcg ggaaaaccgt tgaaggcaaa ctggaccatg
     2341 gcaacagatg aacacatttg ggaccagtcg atccagatca ttgcagatca atttccggaa
     2401 actctctgcc cggctgttcg tctgaccatg ccattaggta atgaccccaa ggggttggaa
     2461 gagcgaacca gtatcatttt cagagaaaca cttgacttgt tgggcgcctg atcaggcagc
     2521 accgatgcgt ttgatcagat tcttgatctg agaatcccac agagctttct ggctgtcaac
     2581 ctcattgggt tcagcaaaat ctgtgatata gagaatagta tctccggtga tctctccctg
     2641 ttctatgcga aactcaaaga attcatcgtc atcataatag tcccagcgga agcgaatgct
     2701 gtcatcgtcg tctgtagtga gcagttctgc ctcttcttcc accccgttcc aggtaaagaa
     2761 gtaggtgtta ccctcattat caacgttatc tgcaaaccac tgtgatagtc cagttggcgt
     2821 gatcagaaaa ttgtggagta cactgggcga ggagcggacg atgtattcca gttcgtattt
     2881 aaccgttttt cccatgtaga tgtcaggttg caagtataac agtttttcaa tgcaaacaaa
     2941 atttttgacc ttttttttaa agtcttgtct tacaatggag ccaaaacccg aaatatcggt
     3001 gacaaaagtg gcttaccggc ttgttcaatg gcaataacca aatagcctca ggagcagaat
     3061 acgaccctca ttttgtacct ttgcagtccg atttctcgac caaaaaattc aatttcaagc
     3121 gtatgagtac caacacctat gagatcaaaa tgaacgagtg gaaccataag gaaatggatg
     3181 ccacagaact gggtatgatc actgttcgtc tctggtttga caaagccata gagctgatca
     3241 ttttccgtcg ctccctggtt gacgccaggg gtagtgagat actcaaccat cacctctatg
     3301 cccagcgttt cctgaataag ccgctgacca ttgatgtgac cctgtccatt gcccgtgaaa
     3361 tggaaaagct ggaccttgct cctgcccgta tcgatctggg tcgcctggga tctgagtggc
     3421 tggaagaaaa agacaattat gacagcatct cctcttttct gcaggataag ctgaaagatc
     3481 ttattggtca ggacaagatc aacctgcagc cacgtgatgt ggtgctgtat ggtttcggac
     3541 ggatcggtcg tattgccgca cgtattctga tcaatgagac aggaaagggc agtcagcttc
     3601 gtctgcgtgc catcgttacc cgttccaaca gtgatgaaga catcctgaaa agagcttctc
     3661 tgctgagaaa ggacagcgtg catggtaaat ttcatggtac cattgtagaa gacctggtca
     3721 acaaagcgct gatcataaat ggtgtgaccg ttcacatgat cgctgccaat aagccggaag
     3781 acatagacta taccgagtat ggcatcaacg atgcgctcat cattgacaat accggggttg
     3841 cacgcgatcg tgaaggtctg ggcaagcaca tgctgagcaa aggcgcttcc atggtgctgc
     3901 ttacagctcc gggaaagggt gatatcccga acatcgtatt cggtgtgaac catgaagaat
     3961 acaatgcaga gaatgaaaag atcttctctg ctgccagttg taccaccaat gcagtagttc
     4021 ctgtcatcca tgtgatggag aacaccttcg gcattgaccg ggggcatatt gaaactgtac
     4081 actcctatac caatgaccag aacctgctgg acaactacca caagaaatac cgacgtggtc
     4141 gttctgctcc gctgaacatg gtcatcacag aaaccggcgc cgccaaagca gtgaccaagg
     4201 tgattccttc catggatgga aaactgacag gaaatgccat ccgcgttccc acaccgaacg
     4261 tctccatggc cattctgaac ctcactctga agaacggtgt aacggtagaa agtgtcaatg
     4321 aagcactgaa ggaagcatcc attcacggca acctgatcga gcagatagac tacagcctga
     4381 gccaggaaat ggtgagtact gactgtgtgg gcaacggaca tgcggtggtt gtagatgcac
     4441 cggccaccat tgtttctgct gacggaaaga gtgtggtact ctattgctgg tatgacaatg
     4501 aattcgggta caccaaacag gtggtcaggt tatccaaata cctgagtggt gttattcgtc
     4561 tgcgttatta ttgatagacc aatcctgatc aaagaccatg tttgaaagtt tatcgaatcg
     4621 tctggagtct gcttttcagt cgctgaaagg ccaggggcgc atcaccgaga tcaatatcgc
     4681 cactacggtg aaagagatca ggcgtgccct ggtggatgcc gatgtcaact ataagatagc
     4741 caaggaattt accgatacgg tgaaggaaaa agcactggga ggtgatgtac tgaaatctgt
     4801 atctcccggg cagctgatgg tcaagatcgt ggaagacgag ctgaccaccc tgatgggtgg
     4861 cacctctacc gatatcaacc tgaaaggatc gccggcggtc attctcatag cgggtctgca
     4921 gggttcgggt aagaccactt tcagcgccaa gctggccagg tacatcaaat caaagagcgg
     4981 gcgtaacccc atgctggttg cctgcgatgt gtatcgtccg gccgccatca accagctgca
     5041 ggtgttgggt gaacagatcg gagttcaggt gtatgcagat acggagaaca agaacccggt
     5101 agatattgct aaaaaggcca tcagctttgc caaagtgaat aaccacaatg tggtgatcat
     5161 agataccgcc ggacgtctgg cggtagatga ggagatgatg gatgagatca cccggatcaa
     5221 acaggctgta caacctgctg agaccctgtt cgtggtagat tccatgaccg gtcaggatgc
     5281 ggtcaacact gcaaaggcct tcaatgaccg gcttgatttt gatggtgtgg tactgaccaa
     5341 gctggacggt gatacccgtg gtggtgcagc actgaccatc aagtatgtgg tcaacaagcc
     5401 catcaagttc atcagtcagg gcgagaagac agataccctg gatgtgttcc acccggatcg
     5461 tatggcccgc cgcatcctgg gcatgggtga tattgtttca ctggtggaga aagcccagga
     5521 acagtatgat gcagaacagg cggcagagct gcagcgaaag atccgcaaga ataaatttga
     5581 ctttgatgat ttcctgagtc agatcggaca gatcaagaaa atgggaaaca tcaaagacct
     5641 gatgggcatg attcccggtg ttggaaaaca actcaaggat ctggacgtgg atgaaaaaca
     5701 attcaagcgt ctggaagcca tgatccagag catgaccccg gcagaacgac aggatcctga
     5761 tctgctcaac ggtgcccgca gaaagcgaat tgctgatggc agtggtactt ccatccagga
     5821 tgtcaatcag ttcatcaagc aattcgaaga gatgcgcaag atgatgaaga tgatgaacaa
     5881 aatgggaggc ggtgctaaag cagccaaggc tctgggtatg atgggacgcc gctagatcag
     5941 cctgcggtaa gcatccatag cagtt
//
LOCUS       DAOMAX010000162         5944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2173_length_5944_cov_4.843946, whole genome shotgun sequence.
ACCESSION   DAOMAX010000162 DAOMAX010000000
VERSION     DAOMAX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2173_length_5944_cov_4.843946"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1509)
                     /locus_tag="PK742_08785"
     CDS             complement(52..1509)
                     /locus_tag="PK742_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQU76723.1"
                     /translation="MRAAIFLFVFLPLVSLAGTYTVDDVPNPKRQSLSNYVSDPDHYL
                     TGDEISTINSMLLRLEDSTTAQVAVVVLESIDYLVPKEFATELFRKWGIGQADVNNGL
                     LILMVIDQRRMEFETGYGLEPVLTDGVCKRIQVERMVPRAKEGRYGQALIDGIEEVNR
                     ILMDPRYREEILSTTDAYGNDTRSFLRKPVSPGWLIGLGLPWILFQWLGWNSEKKGIK
                     KAPAYVKSNFSSMTSASRRTFLGVGLPAALVSSQLMTGMLRVGEFLLILYGILILLTI
                     IKRYRLNHWITQYTEGKEAYTVHQQMQQSHSNGWGAAAIFMPVPMLGYWFWYKRNQHK
                     LRNTPPLSSSGIPMEKLDEKSDDAYLKDVQITEEKIKSIDYDVWLDRSSGETTVYRYD
                     LGKSAYTTCKNCGGKTRKHTKTETLVAATYTSTGKGQKTYTCKHCGDVTYSTFTIAMK
                     TASSGSGSGGSGGGGGGGGSFGGGSSGGGGAGSSW"
     gene            complement(1563..3131)
                     /locus_tag="PK742_08790"
     CDS             complement(1563..3131)
                     /locus_tag="PK742_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQU76724.1"
                     /translation="MRKVLLTGALSLLIAGAFAQERLADPRVTYASTITATELKEHLE
                     VLASDDFEGRETGEKGQKMAAEYISETYKINGVNTTGKINGYFQTFGLTEVTWGDRFI
                     QAGENTYRFFDDFYGFLNQNRDADINTDEIVFLGYGIHDKEYSDYEGKNVANKVIVIL
                     DGEPMRNGISVITGTETRSEWSAGNADKIQVAVEHGVSAVLIIDSKFQLRMDNERWRG
                     WIDHGSMELKESYQEKALLNPLFISRAMADDLLGSKLAAAEKQINKKGKPRSFVIEKK
                     IRVKITKLTEDVFSENVAGFVEGSDLKDEIVVVSAHYDHIGIIDGEVYNGADDDGSGT
                     VAVMEIAQAMQLAKEAGNGPRRSILFLHFAGEEKGLLGSEYYAEHPIFPMEQTVADLN
                     IDMVGRIDDAHAKDSNYVYIIGSNFLSTQLHEINEYNAEHYTKLNLDYTYNSTDDPNR
                     FYYRSDHYNFAKFGVPVIFFFNGTHADYHGAGDEVDKISFNLLEQRARLVFQDLWTLA
                     NMDERIVVDVQQED"
     gene            complement(3142..3585)
                     /gene="rpiB"
                     /locus_tag="PK742_08795"
     CDS             complement(3142..3585)
                     /gene="rpiB"
                     /locus_tag="PK742_08795"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQU76725.1"
                     /translation="MEQQTIAIGCDHAGYDYKEAIAAHLSEAGYLVLDMGTHSSESVD
                     YPDFVHPVAEAIEQGKANLGVLICGSANGVAITANKHQQIRAAIAWQEELASLARQHN
                     NANVICLPARFISQDLAVACVDTFLQSEFEGGRHQRRVDKMTSFC"
     gene            complement(3602..4435)
                     /gene="tatC"
                     /locus_tag="PK742_08800"
     CDS             complement(3602..4435)
                     /gene="tatC"
                     /locus_tag="PK742_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQU76726.1"
                     /translation="MARKNRNEMSFIEHLEDLRWHIIRTLVVIVACSVFFLIRPDIIF
                     DKVILGPKDLNFPTYQVMCALGERLSLNGLCIDELNYDLFYLNISSPFFLYLKIAIMG
                     GAIFAFPYILWEIWRFVRPGLHLHEKRNLGGLVFVATLLFYIGCFFGYFLLAPFSINF
                     LANFELSDQVKNQFTFDSYLGILTGMVLWTGVIFEIPMIAYFLARMGLLTNKFMMQQR
                     KFAFVASLVLAAVITPSGDAFSLFLVATPLWALYEVSIIVVRRVEKRMAKEEGSDASP
                     A"
     gene            complement(4443..5459)
                     /locus_tag="PK742_08805"
     CDS             complement(4443..5459)
                     /locus_tag="PK742_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU76727.1"
                     /translation="MKLNRLYLIIPAMALMAVGCVPSRQYQDAVTARDNYKSEVELLN
                     ASLTQKDKQISMLEDQLEETSDSLRRAVRDFNDLQGAYDRLERANQELREIESLLNSR
                     IKEILAISTTENQELNEKLNAREQEVVQKELALKEKESALEKQRADINALLASIADKE
                     KRIAELESAIKNLNDKLTEVRTSLENALSGFSSSDLSIEQREGRIYINISEKLLFASG
                     STTVDPKGKEALKQVASALKTQDKVSIMVEGHTDNVPLKSANFPKDNWDLSVLRATTI
                     VKLLTADYGLDPVMVEAAGRGEFSPKASNSDAAGRALNRRTEIIIIPDIQEINDILNQ
                     LSGE"
     gene            complement(5465..>5944)
                     /locus_tag="PK742_08810"
     CDS             complement(5465..>5944)
                     /locus_tag="PK742_08810"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQU76728.1"
                     /translation="IKMTAENYPLTEFYDTDEVLTKMGIGEALITALNEKGIPTPLAH
                     TLLVAPQSRMDVLNEDEIRGVITMSSMVKKYNEEVDRESAYEMLTKKLEKAAEQAAKE
                     ADREERNSGRTRTTTTSSRSRRKPKSTMEQVTSSKAFNTAVRELSRGLLGILTKSLK"
BASE COUNT         1337 a         1596 c         1404 g         1607 t
ORIGIN      
        1 cactactcac tactcactac tcactactca ctactcacta ctcactactc actaccaact
       61 gcttcccgca cccccaccgc cggagctgcc gccgccgaag ctgccgccac cgcctccgcc
      121 accaccactt cctccgctac cactgccaga actggctgtc ttcatagcga tggtaaatgt
      181 ggaataggta acatcgccgc aatgtttaca ggtataggtc ttttgtcctt ttccggtgct
      241 ggtataagtg gctgccacca gggtctctgt cttggtatgc ttcctggtct tgccgccaca
      301 attcttgcag gtggtataag ccgacttgcc cagatcgtat cgatagacgg tggtctctcc
      361 ggaggagcgg tccagccaga catcatagtc gatggacttg atcttttctt cggtgatctg
      421 cacatccttc agataagcat catcactttt ctcatccagt ttttccatgg gtatgccact
      481 gctgctgagg ggcggcgtgt tgcgcagttt atgttgattg cgcttatacc agaaccagta
      541 acccagcata ggcacaggca tgaaaatagc cgcagcgccc cagccattgc tgtgcgattg
      601 ttgcatctgc tggtgtaccg tgtaggcctc tttcccttcg gtgtactggg tgatccagtg
      661 attgagacga tagcgtttga tgatggtcag caggataaga atgccgtata gaataagcag
      721 gaactcccca accctcagca tgccggtcat cagctgtgag ctgaccagtg cagcgggcag
      781 tcccactccc agaaatgtgc gccgggaagc agaggtcatg ctgctgaaat ttgatttcac
      841 ataagcagga gcttttttga tgcctttctt ctcgctgttc catcccagcc actgaaagag
      901 tatccaggga agtcccaggc cgatcaacca tcccggcgac accggtttgc gcaagaaact
      961 tctggtatca ttgccatagg catcggtggt ggacaggatc tcttcgcggt atcgtggatc
     1021 catcaggatg cggttcactt cttcgatgcc atctatcaga gcctgaccgt atcgtccttc
     1081 tttagcccgg ggtaccatgc gttccacctg tatgcgcttg catacgccat cggtcaaaac
     1141 gggttcgagt ccgtatcccg tctcaaattc catgcgtcgc tgatcgatga ccatgaggat
     1201 aagcaggccg ttgttgacat ccgcctggcc gatgccccac ttccggaata gctcggtagc
     1261 aaattctttg ggcaccaggt agtcaatgga ctccagcacg accacagcca cctgtgccgt
     1321 ggtactatcc tccaggcgaa gcagcatact gttgatcgta gagatctcat caccggtcag
     1381 gtaatggtcc ggatcgctca catagttgct gagcgactgg cgtttgggat tgggtacatc
     1441 atccaccgtg taggtgccgg ccagcgacac caggggcagg aatacaaaca ggaagatggc
     1501 agctcgcata ggtaaacaaa tatacgattc accttaacca aaagctaccg acctgagaat
     1561 gctcagtcct cctgctgcac atccaccaca atacgctcat ccatattggc cagggtccag
     1621 aggtcctgga agacaagacg ggcacgttgt tccagcaggt tgaagctgat cttgtccacc
     1681 tcatcgccgg cgccgtgata atcagcatgg gtgccgttga agaagaagat gaccggcaca
     1741 ccgaatttcg caaagttgta gtgatcactg cggtagtaga atcggttcgg atcgtcggtg
     1801 gagttatagg tgtagtcgag gttcagtttg gtataatgtt cggcattgta ctcattgatc
     1861 tcatgcagct gggtgctcag aaaattggaa ccgatgatat agacatagtt gctgtctttg
     1921 gcatgggcat cgtcgatgcg acctaccata tcaatgttca ggtcagccac ggtctgctcc
     1981 atcggaaaga tgggatgttc ggcatagtat tcagaaccca gcaggccttt ttcttcacct
     2041 gcaaagtgca ggaacaggat gcttcttctg gggccatttc cggcctcttt ggccagctgc
     2101 atggcctggg caatttccat gaccgccacc gtacccgagc catcgtcatc ggcaccatta
     2161 tagacctcac catcaatgat accaatatga tcataatgtg cagacaccac tacgatctca
     2221 tctttcagat cgctgccttc cacgaaacct gccacattct cagagaacac atcttcggtt
     2281 agcttggtga tcttcacccg tatctttttc tctattacaa atgaacgtgg cttccccttt
     2341 ttattgatct gcttttctgc agcagccagt ttgcttccca gcaaatcatc tgccattgcc
     2401 ctgctgatga acaggggatt gagcagggcc ttttcctgat agctctcctt gagttccatg
     2461 ctgccatgat cgatccatcc cctccatctt tcattgtcca tgcgcaactg aaacttactg
     2521 tcaatgatca gcactgccga aactccatgc tctactgcaa cctggatctt atctgcattt
     2581 cctgctgacc actcacttcg ggtttctgtt ccggtaatta cagaaatgcc attacgcata
     2641 ggttcaccat ccagtatcac gatgacctta tttgccacat tcttgccttc ataatcactg
     2701 tactctttgt catggatgcc atatccaaga aacacgatct catcggtatt gatatcagca
     2761 tcccggttct ggttcagaaa accatagaag tcatcaaaaa agcgataggt gttctctcct
     2821 gcctggatga aacggtcgcc ccatgtcact tctgtcaagc cgaatgtctg gaagtatcca
     2881 tttatttttc ctgtcgtatt tactccattt attttatatg tttcactgat atattctgca
     2941 gccattttct ggcccttctc tcctgtttcc cggccctcaa aatcatccga ggcaagcacc
     3001 tccaggtgct cctttaattc agtggcggta atagtggatg catacgtcac tcttggatct
     3061 gccagacgct cctgggcgaa ggcgccggca ataagcaggg acagtgcccc tgtaagcaat
     3121 acttttctca ttggtttggt cttaacagaa actggtcatt ttatctacac ggcgctggtg
     3181 acggccgcct tcaaattcgc tttgaaggaa tgtatctaca caagcgacag cgagatcctg
     3241 tgaaatgaaa cgtgccggca aacagatcac attggcgttg ttgtgttgtc ttgccagcga
     3301 tgccagttct tcctgccatg cgatggctgc ccggatctgc tggtgtttgt tggcggtgat
     3361 ggccacaccg tttgcagaac cacagatgag cacacccagg ttggcttttc cctgctcgat
     3421 ggcttccgct acagggtgca cgaaatcggg ataatctact gattcagaag aatgggtacc
     3481 catgtccagc accaggtatc cggcttcact cagatgggct gcaatggctt ctttatagtc
     3541 gtaaccggcg tgatcacaac cgatggcgat ggtctgttgc tccatgtgtt aaagatacac
     3601 atcaggcagg agatgcgtca ctcccttctt cctttgccat gcgcttctct acccggcgca
     3661 ccacaatgat gctgacctca tacagagccc aaagcggagt agctacaagg aacaggctga
     3721 atgcatcgcc ggagggtgtg atgacagcag ccagcaccag cgatgccaca aaggcgaact
     3781 tgcgctgctg catcatgaac ttgttggtaa gcaggcccat tctcgccagg aaataagcga
     3841 tcatcggaat ctcaaaaatg acgccggtcc acaacaccat gcctgtcaga attccgagat
     3901 aggaatcaaa ggtgaactgg ttcttcacct gatcgctcag ttcaaaattc gccaggaagt
     3961 tgatgctgaa gggtgccagt aaaaagtaac cgaaaaaaca gccgatataa aacagcagtg
     4021 tcgccacgaa taccagtccg cccaaattcc gtttctcgtg caggtgcaat cccggcctta
     4081 caaaacgcca gatctcccag agtatgtatg gaaaggcaaa gatggctccg cccatgatag
     4141 cgatcttcag gtataaaaag aagggtgaac tgatgttgag atagaagaga tcgtagttca
     4201 gttcgtcaat gcagagacca ttcagactga gtctttctcc aagtgcacac atgacctgat
     4261 aggtggggaa gttcaggtct ttcggaccca gtatgacctt gtcaaaaatg atatcgggcc
     4321 ggatcagaaa aaagacggag catgccacga tcaccacgag ggtgcggatg atatgccacc
     4381 gcagatcctc gagatgctcg ataaaagaca tctcatttct gttctttctg gccatgcctg
     4441 ttttactcgc cgctcagctg attgagtatg tcgttgatct cctggatatc aggtatgatg
     4501 atgatctctg ttctgcgatt gagtgccctt ccggctgcat cagaattgct tgccttgggc
     4561 gagaactcac cacggcctgc tgcttctacc atcacaggat cgagcccata atcagctgtc
     4621 agcaatttga cgatggtggt agcacgcagc acagacaggt cccagttgtc tttcggaaag
     4681 ttagcactct taagtggcac gttatccgta tgaccttcta ccatgatgga aaccttatcc
     4741 tgggtcttca gtgctgaagc aacctgtttc agtgcttctt ttcctttcgg atcgacggtt
     4801 gtactgccgc tggcaaagag cagtttctcc gaaatgttga tatagatacg tccttctctt
     4861 tgttcgatgc tgaggtcgga ggaactgaag ccactcagtg cattttccag actggtgcgc
     4921 acctccgtca gtttgtcgtt cagattctta atggcagact ccagttcagc gattcgtttt
     4981 tccttatcag ctatggaggc cagtaatgca ttgatatctg cccgctgttt ttccagagct
     5041 gattcctttt ctttcagggc cagttctttt tgcactactt cctgttcacg ggcattcagt
     5101 ttctcattca gttcctggtt ctcggtagta ctgatggcca ggatctcctt gatgcgactg
     5161 ttcagcagac tctcgatctc ccgcaactcc tgattggctc tctccagcct gtcatatgct
     5221 ccctgcaggt cgttgaaatc gcgaacggca cggcgcagac tgtcggacgt ttcttccagc
     5281 tgatcttcca gcatacttat ctgcttatcc ttttgcgtca gagatgcatt cagcagctcc
     5341 acttcgcttt tgtagttatc gcgggcagtt accgcatcct ggtattgacg cgagggcaca
     5401 cagccaacag ccatcagtgc catcgccgga atgatcaggt acagtctgtt cagtttcata
     5461 tggttcattt taaactttta gtgaggatgc ccaggaggcc gcggctcagc tctcttacag
     5521 ctgtgttgaa tgccttgctg ctggtaacct gttccatagt agattttggt tttcgacgag
     5581 acctcgaaga cgtggtggtg gtcctggttc tgccgctgtt tcgctcttct ctatctgctt
     5641 cttttgctgc ctgctcagct gccttttcca gctttttggt cagcatctca taggcactct
     5701 cgcggtccac ttcttcattg tactttttca ccatcgaaga catggtgatg accccgcgga
     5761 tctcatcttc attcaatacg tccatcctgc tttgtggtgc caccagtaag gtatgtgcca
     5821 gcggagtggg aatacctttt tcattcaggg cggtgatgag tgcctcacca attcccattt
     5881 tggtcagtac ctcatccgta tcatagaact cggtgagggg ataattctct gccgtcatct
     5941 tgat
//
LOCUS       DAOMAX010000163         5918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2186_length_5918_cov_4.481835, whole genome shotgun sequence.
ACCESSION   DAOMAX010000163 DAOMAX010000000
VERSION     DAOMAX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2186_length_5918_cov_4.481835"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK742_08815"
     CDS             <1..534
                     /locus_tag="PK742_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76729.1"
                     /translation="MDICVSNYFKFKTDHENRYSNPRLMKHGLLRRYIMRWWNVRQYK
                     NIIGKDETFEQFFLRAYSVPYASWSILDHDKFDAIIRFENLQGDFDAALSKLGMEKVR
                     DIPVANKTAEKTKSFWEYYETDKAKKRAKYIFGPYLKRWGYDFPESWSKFKTPWYSFA
                     LYRCLNVMRRIFWIYLR"
     gene            534..1508
                     /locus_tag="PK742_08820"
     CDS             534..1508
                     /locus_tag="PK742_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU76730.1"
                     /translation="MKGTQPTFLVVGAVKAGTTSLHEYLQQHPEVYMSPVKETNHFSD
                     ADMDFAHFNVDYRQDIDHNLDKYLAGTMDHKIHIAHVRTAAQYSLLYKNVQDEKAIGE
                     VSNSYLYCPSTAAAIHQALPAVQIVMVLRNPVDRLYSQYLMNLKLGKITEKDLLTEIA
                     HDAAKEHKGWGVSHLYVEVGMYHEQVKRYLDLFPREQVHIMLFDDFKQDVAGAMSDLF
                     GFLGVDPAPGIDYSKRYNEAGMPRFGKLNYWMTQIGLYGLFKKLFPDNLKQRLKSVIY
                     TQKGIPKITSEERKQLEDLYREDVERLSALIGRDLSHWFSQDIEGGDD"
     gene            1608..2249
                     /gene="rpsP"
                     /locus_tag="PK742_08825"
     CDS             1608..2249
                     /gene="rpsP"
                     /locus_tag="PK742_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQU76731.1"
                     /translation="MAVKLRLQRHGRKKAPYYHIVVAPSTAPRDGRFIERLGYYNPTK
                     VPAQIDIDVDKAVEWLDKGAQPTDTVRAILRYKGVLYKKHLMRGVKKGAFDLEAAEAK
                     FADWQKSHENAVMDHVKSVEDKASKAKAEEAARYEAKRQERIAAEQKAVAEAEAAAEA
                     EAVEASAEVAEEVEVTEAAETEAPVAEAATEEAPAVEEAPAAEEAAEEPKTEE"
     gene            2250..2759
                     /locus_tag="PK742_08830"
     CDS             2250..2759
                     /locus_tag="PK742_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76732.1"
                     /translation="MKAIQLAEAGKVIRTHGVDGTLHLLADQPIEELAPGEALFLWED
                     GIRLPFFIVQAEPLEEGEWLVQVEELQSREAARRYAGHPFWLQAHLVGESAEEDSDLV
                     GWKVEDADGKPVGVILSAVEMGEYILLTIDHQGREILLPLHDDLIVSLQEDHRTITLQ
                     IADGLLDLD"
     gene            2763..3467
                     /gene="trmD"
                     /locus_tag="PK742_08835"
     CDS             2763..3467
                     /gene="trmD"
                     /locus_tag="PK742_08835"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQU76733.1"
                     /translation="MLHIDIISVVPDLLESPFSHSIMKRARDRGLLEVDVINLREYAV
                     NKYGQVDDYAFGGGAGMVMMVEPVDRCIADLKVKRNYDEVIYMSPDGELLDQPLANRL
                     SLTGNLIILCGHYKGVDERIRQHLITREVSIGNYVLSGGELAAAVLVDAIGRLLPGVL
                     NDETSALSDSFQDGLVAPPVFTRPADYQGWPVPDVLLSGHEAKIAEWRLQQAIERTKE
                     RRPDIWQDFENQEGKH"
     gene            3508..3858
                     /gene="rplS"
                     /locus_tag="PK742_08840"
     CDS             3508..3858
                     /gene="rplS"
                     /locus_tag="PK742_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQU76734.1"
                     /translation="MDALKTLEMDLIPAKDIPEFKAGDSITVHYRIVEGDKSRIQLFK
                     GDVLQRKGTGRTQTFTVRKISNGVGVERIFPLFSPNIEKIEVNKVGRVRRSRIFYLRG
                     LKGKSARIKEKRQA"
     gene            complement(3906..4589)
                     /locus_tag="PK742_08845"
     CDS             complement(3906..4589)
                     /locus_tag="PK742_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76735.1"
                     /translation="MRPKIYKVLILVATALLVFGSEIANAQEPGYSFNFRQISESEFR
                     ATQRNNYNATTVATLDSIDLEKAFELIDDTYQQDELILAERELCKSPRCLTAFHGLYP
                     NLGILAFFIQDLHYEKVIFLPASLDDSYSSFDRFYGTFGAMSKNGFWVGLKREGSDNY
                     LQAEICKVSDTGTSSILQFKFSLTDINEAENEPIFWVNENSFYLAVIDSNNSEKKDQQ
                     FYEISFNIK"
     gene            complement(4627..5253)
                     /locus_tag="PK742_08850"
     CDS             complement(4627..5253)
                     /locus_tag="PK742_08850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU76736.1"
                     /translation="MPEFWESIYSNNNKMWGEDPTDNARYVLELFRKNNVRSVLIPGF
                     GYGRNARVFHDAGMVVSGIEISATAIERARKYFSEEVIIHHGSVSKMPFDNERFDAVY
                     CWSLIHLLNKADRLKLIENCHAQLEPDGLMVFTALSIYDKRFGEGQEIDKNTFQSPNG
                     LTLYFYDEASIREEFGKYDILELSEIDEPEVNPNERQWMIICKKNSSA"
     gene            complement(5375..5698)
                     /locus_tag="PK742_08855"
     CDS             complement(5375..5698)
                     /locus_tag="PK742_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76737.1"
                     /translation="MDKQKIINKMVSNARAIISNQIALPLGCLKMNRIIVWLNSSQCD
                     IDIDVSVFSLYNLKTKNLPVGTERLYWNIEKLIEFEQELAEIDSLYKADILRICRRLI
                     DRYGT"
BASE COUNT         1584 a         1369 c         1485 g         1480 t
ORIGIN      
        1 atggatatct gtgtaagtaa ttatttcaag ttcaagaccg accacgagaa caggtattcc
       61 aatccccgcc tcatgaagca cggtcttctt cggagataca tcatgcgctg gtggaatgtg
      121 cgtcagtaca agaacatcat cggcaaagac gaaacctttg agcagttctt tctccgggct
      181 tactctgtgc cctatgccag ctggagtatc ctggaccacg ataagttcga cgccatcatc
      241 cgctttgaga acctgcaggg cgattttgat gctgccctga gcaaactggg tatggagaaa
      301 gtgcgtgata ttccggtagc caacaagacc gctgaaaaaa caaagtcctt ctgggaatat
      361 tacgagaccg ataaggcaaa gaaacgggcc aaatacatct tcggacctta tctgaaaaga
      421 tggggctatg attttccgga aagctggagc aaattcaaaa cgccctggta ctcatttgcc
      481 ctgtatcgct gcctcaatgt gatgcgaaga attttttgga tctacctgcg ctgatgaaag
      541 gaacgcaacc cactttcctg gtagttggtg ctgtgaaagc aggcaccacc tcgctgcatg
      601 aatacctgca gcagcacccg gaggtgtata tgtcgccggt caaggagacc aatcatttca
      661 gtgatgccga catggacttt gcgcatttca atgtggacta tcgtcaggat atagatcaca
      721 acctcgacaa atatctggct ggtaccatgg atcacaagat ccacatcgcc cacgtcagaa
      781 cagctgcaca atacagcctt ttgtacaaga atgtacaaga cgaaaaagcc atcggcgagg
      841 tcagtaattc ctatctatat tgtccgtcca ccgccgcagc catccatcag gcattgcctg
      901 cagtgcagat cgtcatggtg ctgcgcaatc cggtggatcg tttgtattcg cagtacctca
      961 tgaacctcaa gctgggcaag atcaccgaga aggacctgct cacagagatc gcccacgacg
     1021 ctgccaagga acataaaggc tggggtgtaa gccatttata tgtggaggtg ggcatgtatc
     1081 acgaacaggt gaagcgctac ctggacctct tcccgcggga gcaggtacat atcatgctgt
     1141 tcgacgattt caagcaggac gtagcaggag ccatgtcaga cctttttggt tttctgggag
     1201 tggatcctgc ccccggcatt gattacagca agcgatataa cgaggccggc atgccccgct
     1261 tcggcaagct gaattactgg atgacccaga tcgggctgta cggattattt aaaaagctgt
     1321 tccccgacaa cctcaagcaa aggctgaaat cggtcattta cacccagaag ggcatcccga
     1381 agatcacctc agaagagcga aaacagctgg aagacctgta ccgggaagat gtggaacgcc
     1441 tttctgcact cataggccgt gatctcagcc actggttcag ccaggacatt gagggcggag
     1501 atgattgatt tgcacagaaa aattccctat ttttgcaccc gcaaatccca ataaccgatt
     1561 cattcggggc tgagggacat tattcacaat aattcaatta ttgcaacatg gcagttaaat
     1621 tgcgtttaca gcgccacggg cgcaaaaaag ctccttacta tcacatagta gtagctccgt
     1681 ccaccgcacc ccgcgacgga cgattcattg aaagacttgg ttactacaac ccgaccaagg
     1741 ttcctgcaca gatcgacatc gatgtggaca aggcagtaga gtggctggac aaaggcgctc
     1801 agcctacaga caccgttcgt gccatcctcc gctacaaagg cgtgctgtac aaaaagcacc
     1861 tgatgcgtgg tgtgaagaaa ggtgcatttg atctggaagc tgctgaggca aaatttgccg
     1921 actggcagaa gagccatgag aatgcagtga tggatcacgt caagagcgtg gaagacaaag
     1981 catccaaagc caaggctgaa gaagctgctc gttacgaggc caagcgtcag gagcgcatcg
     2041 cagctgaaca gaaggctgtc gcagaagcag aagctgctgc agaagcagag gctgtggaag
     2101 ccagtgctga agtagcagaa gaggtggaag tgaccgaagc tgctgaaaca gaagcaccgg
     2161 tagctgaagc tgccacagaa gaagcacctg ctgtggaaga ggctcccgct gctgaagaag
     2221 ctgctgagga acccaagacc gaagaataag tgaaggccat tcaactggct gaagcaggaa
     2281 aggtcatccg cacccacggc gtggacggca ccctgcacct gctggctgac caacctatag
     2341 aagagcttgc ccccggggaa gctctttttt tatgggaaga tggcatccgt ctgccctttt
     2401 tcattgtgca ggctgaaccg ctggaagaag gcgaatggct ggtgcaggtg gaggaattgc
     2461 agagcaggga agcagcccgt cgctatgccg gacacccgtt ctggctgcag gcacatctgg
     2521 tcggggaaag tgctgaggaa gacagcgatc tggtgggatg gaaagtggag gatgcagatg
     2581 gaaaaccggt aggagtgatc ctgtcggcgg tggaaatggg agaatacatc ctgctgacga
     2641 tagaccacca gggcagggag atcctgttgc ccctgcacga tgacctcatt gtatctttac
     2701 aggaagacca tcggacgatc acattacaaa ttgccgacgg tctgctggac ctggactgaa
     2761 ccatgctgca tattgatatc atatcggtgg tgcctgatct gctggaatcc cccttcagcc
     2821 acagcatcat gaagcgtgcc cgcgatcgcg gcctgctgga ggtggatgtc atcaacctgc
     2881 gggaatatgc ggtcaacaag tacggtcagg tggatgatta tgcctttggc ggaggggcag
     2941 ggatggtcat gatggtggaa cctgttgacc gttgcatcgc tgatctgaag gttaaaagaa
     3001 actacgacga ggtgatctat atgtcgcccg acggcgaatt actggaccag cccctggcca
     3061 atcgcctgag cctgacaggt aacctgatca tcctttgcgg gcattataaa ggcgtggatg
     3121 agcggatacg acaacatctg atcacccgcg aagtttccat agggaattac gtgctcagtg
     3181 gcggcgaact ggcagcagcg gtcctggtgg atgccatcgg ccgattgctt cccggggtgc
     3241 tgaatgacga gacatcggcc ctgagcgaca gttttcagga cggactggtg gctccgccgg
     3301 tctttacccg tccggccgat taccagggct ggccggttcc cgatgtactt ttgagcggcc
     3361 acgaggcgaa aattgctgaa tggcgcctgc agcaggccat agagcgtacc aaagagcgca
     3421 ggcccgacat ctggcaggat tttgaaaatc aggaagggaa acactaactt tgcaggcctg
     3481 ttacaggaaa tttaaaaaga tcagacaatg gatgcgctga agacactgga gatggacctc
     3541 atccccgcaa aagatatccc ggaattcaag gccggcgaca gtattactgt tcactaccgt
     3601 atcgtggaag gcgacaagag ccgtatccag ctgttcaagg gcgatgtcct gcagcgcaaa
     3661 ggcactggtc gtacacagac cttcactgta aggaagatct ccaatggtgt gggtgtagaa
     3721 cgtatcttcc cgctgttctc accgaatatc gaaaagatcg aggtgaacaa ggtaggtcgt
     3781 gtgcgcagaa gccgtatctt ctacctccgc ggactgaaag gtaaatctgc ccgtatcaag
     3841 gaaaagcgtc aggcttaaga tatctataat gtatagtgta aagccacctg cgagggtggc
     3901 ttttcttatt tgatgttgaa agatatttcg taaaactgct gatctttctt ttcagaattg
     3961 tttgaatcaa taaccgctaa atagaatgag ttttcattga cccagaatat aggctcattt
     4021 tcggcttcat tgatgtctgt cagcgaaaac ttgaattgca gtatagagct ggtaccagta
     4081 tcggatacct tgcaaatttc agcttgcaga tagttgtctg atccttctct ttttaaccca
     4141 acccagaagc cattttttga catggcgccg aaggtgccgt aaaaacgatc aaaagagctg
     4201 taagaatcat ctaaacttgc cggcaggaaa atgacttttt catagtgcag gtcctgaatg
     4261 aaaaaagcga gaatgccaag atttgggtag agtccatgaa aagcggtcag gcatctggga
     4321 gatttgcaca attcacgttc tgcaagaatg agctcatctt gttggtatgt atcatcaatt
     4381 agctcaaagg ccttttcaag atcaattgaa tctaaagtcg caactgtagt tgcattgtaa
     4441 ttattccttt gtgtagctct gaattcggat tcgctgatct ggcgaaaatt aaatgaatac
     4501 ccaggctcct gcgcattggc tatttcgcta ccaaacacca ggagtgcagt tgccacaaga
     4561 atcaagactt tgtagatctt aggtcgcata aaaagactca gtctaactga ccgacattct
     4621 tcataatcaa gctgaactat tttttttgca aatgatcatc cactgtcttt cattgggatt
     4681 cacttccggt tcgtcgatct cgctaagttc taagatatca tactttccga attcctccct
     4741 tatggatgct tcgtcgtaga agtaaagggt caggccgttc ggcgattgaa aagtgttctt
     4801 gtctatttct tgtccttcac caaatcgttt gtcataaatg gataatgcag tgaagaccat
     4861 cagaccgtcg ggttccagtt gggcatgaca gttttcgatc agttttagtc tgtccgcttt
     4921 gttgagcagg tgaatgagcg accagcaata gaccgcatcg aagcgttcgt tgtcaaaagg
     4981 cattttgctg accgaaccgt ggtggatgat cacctcctca ctgaaatact tccgggctct
     5041 ttcaatggcc gttgcggaga tttcaattcc tgaaactacc atgccggcat cgtgaaagac
     5101 cctggcattt ctgccatagc caaatcccgg tatcaggaca ctcctgacat tattttttct
     5161 gaacagctcc agtacatacc gggcattgtc tgtggggtct tctccccaca tcttgttatt
     5221 gttgctgtag atggattccc agaattcggg catggggaag ttatttgttg ttcgaatttg
     5281 tggtttaccg tgctgaatat tgaatgataa attcagcaag gatatcatgt ctcctatatt
     5341 ctagatctat ttctgtgctt ggtttatctg aattctaggt gccataccta tcaataagtc
     5401 ttctgcaaat tctaagaata tcggctttat agagagaatc gatctcagcc aattcctgtt
     5461 caaattcaat cagtttttca atattccaat acaatcgctc ggtaccgacc ggtaaattct
     5521 ttgttttgag gttatataaa ctgaagacag aaacatcaat gtctatatca cattgggatg
     5581 aatttagcca aactattatt cgattcattt tcaaacagcc aagaggaagt gctatttgat
     5641 ttgatataat tgcccgtgca tttgatacca ttttgttgat gatcttttgt ttatccattt
     5701 tacttattgg aagaaaaccg tgtatgatac aaagcggatg gatgtattgc cataaccttg
     5761 tcccatattt ttacttttat ttgttaataa gttgattcaa gtttatagtt cctctgtaga
     5821 cttccgagaa attgtttccg atcttcagcc taagatctgt ctcataatcc ccagcatata
     5881 tagtagctga tgtcaggatg acatctgcgg gcggcaag
//
LOCUS       DAOMAX010000164         5890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2199_length_5890_cov_3.780805, whole genome shotgun sequence.
ACCESSION   DAOMAX010000164 DAOMAX010000000
VERSION     DAOMAX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_5890_cov_3.780805"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PK742_08860"
     CDS             <1..303
                     /locus_tag="PK742_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76738.1"
                     /translation="SLVRKYLGLDQVVENLVRRLGDVHLAIVTGDYAKGKDSGTIDLL
                     VVGNIDTVLLDYLVQKTEMIINRKILPTVLDQAGFQIWKQQHDLQEAIVLWNGQKF"
     gene            437..1750
                     /locus_tag="PK742_08865"
     CDS             437..1750
                     /locus_tag="PK742_08865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQU76739.1"
                     /translation="MKLTVVGTGYVGLVTGTCLADVGVEVHCIDIDAAKIERLRAGEV
                     PIYEPGLDLLLKNNLDKGKLHFSTSLKESLPGSDVAMIAVGTPQGEDGSADLQYVIRV
                     AEEIGMAMDRELVVVTKSTVPVGTHKKVDQAIRSQLEKRGVEIPFDVVSNPEFLKEGA
                     AVADFLKPDRIVIGYTGERARSIMHKLYQPFVLNGHPIIDMDPASAEMTKYAANAMLA
                     TRISFMNDIANLCEKMGADINDVRKGIGSDPRIGSQFIYAGIGYGGSCFPKDVKALIR
                     MGQEQGYPMQVLEAVDGVNNRQKQVLSNKIFDRFGKDLTGKTFAVWGLAFKPKTDDMR
                     EAPALTIISELVAAGASVLAYDPVAMQVARPLLPEGVTLTDNALTATAGADGLLLVTE
                     WPEFRLPDWQQVKKNMKQAVVFDGRNIYDPEEMQQLGFVYEAIGR"
     gene            1769..2116
                     /locus_tag="PK742_08870"
     CDS             1769..2116
                     /locus_tag="PK742_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HQU76740.1"
                     /translation="MNKEHDIRPWGAYWVLEDTGTYKVKRIEVEPGHQLSYQYHHHRS
                     EVWVIIEGHAEVILEGEVIDCPPGKVVQIPQGARHRIRNAGNEKVVFVEVQLGSYFGE
                     DDIVRLDDDYERA"
     gene            2126..3391
                     /locus_tag="PK742_08875"
     CDS             2126..3391
                     /locus_tag="PK742_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQU76741.1"
                     /translation="MEGKNKTVAVIGLGYVGLPLAVELGKRYTTIGYDINRERIEELQ
                     KGEDRTLEVSREELQSASALTLTNDLSPVGEAQIFIITVPTPVDQSKSPDLTPLIGAS
                     DMIGRLLKKGDIVIYESTVYPGCTEEVCVPVLEHASGLKYNEDFFCGYSPERINPGDK
                     VNTLTKIKKVTSGSTPEVAQEVDALYASIIEAGTHLAPSLKVAEASKAIENAQRDLNI
                     SFMNELALIFERIGIDTNDVLEAAGTKWNFLRFKPGLVGGHCIGVDPYYLVHKAESVG
                     YHPQVILSGRRVNDNMGVYVANRLVKRMISKGVTIKGNRVLILGIAFKENCPDTRNTK
                     VVDIYNELREFGLEVDVYDPWVDPKEVMHEYGISLLEEEPKGPYAGILVAVAHRQFAE
                     MNLSALKANEAVVFDTKAFLDRSLVDERL"
     gene            3396..4883
                     /locus_tag="PK742_08880"
     CDS             3396..4883
                     /locus_tag="PK742_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HQU76742.1"
                     /translation="MSLKKQAFSGFVWTFTSNLGTKFTTLVVGVILARLLAPSDFGLI
                     AMLYFFFEVSRVLVDSGFSQAIIREKEINEEDKSTVFYISLFTAVICYIALWIGAPAI
                     ARFYEEPQLTLLTRVMGLNLIFASFTIVQNAVLINELKFKTLGIIAMAANVLTGLIAL
                     FLAWSGMGVMALAIRYVLTSLITSILYFAVLTWYPKVFIQKRSLKKLFGFGSRVLLLG
                     VMNTFFRNIYNLLIGKIYTPAILSFYSQANMFKENASRGLIDSLSKVTYPILSKDRDN
                     RARMKENYRKILQVTSFVVFPSMILLAFVAEPMILALVGEKWRQSIPLLQILCVAGLV
                     SHIHVVNLNVLKVYAAGNQYVLQGIIKYALTVAGVLVGMQFGLSGLMYGIVVVEYLHL
                     LVNIYLSNQHLSYTYSEQGLDLLKVFWLNLPMAGVLWLTSLPEYPAPVFQLLVMTGTG
                     LVMYILTAHLTNAMAWKYMVELIQGQLRKRKISTAAETTAHVETA"
     gene            5083..5640
                     /locus_tag="PK742_08885"
     CDS             5083..5640
                     /locus_tag="PK742_08885"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU76743.1"
                     /translation="MLKRDIPASSKRWCEKSPPNVRYLEQLFREFGRDLKVILMIRDG
                     RDVITSMHPLREGYYIPIERWINDTRQTIRWKDHPQVLLVPYESLVSNFRPVIEQVLT
                     FLEAAMSQEVLDYTNYSGVQQHDAFHGQHLRPLYTASQRKWELPEHRERIDLFLQNEE
                     VQELMNSIVDIRQAFDLSLENVERA"
     gene            5637..>5890
                     /locus_tag="PK742_08890"
     CDS             5637..>5890
                     /locus_tag="PK742_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76744.1"
                     /translation="MKILIISKFFYPSVTPRSFRTTALAKAFAKAGHEVQVYTSWNEE
                     IHPAYAVEHGMEIHPLSKWIWKDIRADKGAGPFRLVRRIL"
BASE COUNT         1597 a         1266 c         1565 g         1462 t
ORIGIN      
        1 agcctggtgc gaaaatacct gggactggat caggtggtgg aaaatctggt aagaagactg
       61 ggcgatgtgc acctggccat tgttacaggc gattatgcca aaggaaagga cagcggaacc
      121 attgatctgc tggtggtagg aaatattgat accgtattgc tcgattatct cgtgcagaaa
      181 acggaaatga taattaaccg gaagatactg cccacagtgc tggatcaggc cggttttcag
      241 atatggaaac agcagcacga tctccaggag gcaatcgtgt tgtggaatgg tcagaagttc
      301 tgacccagcc atctgctgtt gatgccggtc gcaaaaaaaa gacatgtgac tgagagggcg
      361 aagctgtgcc cggacactga aaaacaagag ataaatgggg tgaagaggct aactttgccc
      421 ctctaaaatt gcagacgtga aactgaccgt tgttggaaca ggatatgtgg gattggttac
      481 cggaacctgt ctggctgatg tgggcgtgga agtccactgt attgatattg atgcagccaa
      541 aattgaaagg ctcagggccg gcgaagtgcc tatctatgag cctggactgg acctgttgct
      601 gaagaacaac ctggacaaag gaaaactcca cttctctacc agtctgaagg aaagccttcc
      661 gggaagcgat gtggccatga tagcagtggg aacaccacag ggtgaagatg gcagtgcaga
      721 tctgcagtat gtgatccgtg tggcagaaga gatcggtatg gcaatggacc gtgaactggt
      781 agtggtcacc aaaagcacgg tgccggtagg tactcataaa aaagtggatc aggccatcag
      841 gtcacagttg gaaaaaagag gtgtggaaat tccattcgat gtggtatcca atcctgaatt
      901 cctgaaagaa ggtgccgccg tggcagattt tctgaagccc gaccgcatcg tgatcggtta
      961 taccggcgag cgtgcccgta gcatcatgca taagttgtac cagccgttcg tactcaatgg
     1021 tcatcccatt attgatatgg atccggcatc ggcagagatg accaagtatg cagccaatgc
     1081 tatgctggcc acccgcatca gtttcatgaa tgatatcgcc aacctgtgcg aaaaaatggg
     1141 tgctgatatc aatgacgtga gaaagggtat tggaagtgat ccgcgtatcg gttctcagtt
     1201 catttatgca ggtattggtt atggaggttc ctgctttccc aaggatgtaa aggctctgat
     1261 cagaatgggg caggagcaag ggtacccaat gcaggtactg gaagcagttg acggtgtcaa
     1321 taaccggcag aagcaggtgc tgtccaacaa gatatttgac cggtttggca aagacctgac
     1381 cggaaagaca ttcgcagtct ggggcctggc tttcaaaccc aagacagatg atatgcggga
     1441 agctcctgca ctcaccatca tcagtgagtt ggttgctgca ggtgccagtg tactggcgta
     1501 tgatccggtg gctatgcagg tagcaagacc tttgttgcct gaaggcgtta cccttaccga
     1561 caatgcactc actgccacag ccggtgccga tggattgttg ctggtaaccg aatggccgga
     1621 gttccggctg ccggactggc aacaggtaaa gaagaatatg aagcaggcag tggtgtttga
     1681 cggacgtaat atttacgatc cggaagagat gcagcaattg ggctttgtat atgaagccat
     1741 cggacgatga tcccgtaaca agcagaccat gaacaaggaa catgatatca gaccatgggg
     1801 cgcctattgg gtattggaag ataccggtac ttataaggtg aagcgcattg aggtggaacc
     1861 tggtcaccag ttgtcatacc aatatcacca tcaccgttca gaggtatggg tcatcattga
     1921 aggacatgct gaggtgatcc tggaaggtga ggtcatcgat tgtcctcccg gtaaagtggt
     1981 gcagatacca caaggtgcca gacaccggat caggaatgca ggaaatgaaa aggtggtgtt
     2041 tgtggaagtg caactgggtt cctatttcgg cgaagatgat atcgtgcgtc tcgacgacga
     2101 ttacgaaagg gcttaacaaa agaacatgga aggaaagaac aagacggtcg cggtcattgg
     2161 cctcggctat gttggcctgc ccctggcagt ggaattaggc aagagatata caaccatcgg
     2221 ttatgatatc aaccgggagc gcatagaaga actgcagaaa ggagaggatc gtacgttgga
     2281 agtgagcaga gaagaattgc agtctgcctc cgcactgacc cttaccaatg atctgtcgcc
     2341 ggtgggtgaa gcacagatct tcatcataac cgttcctact cctgttgatc aaagcaagag
     2401 tccggatctg acaccgctga tcggcgcttc tgatatgatc ggccgattat tgaaaaaagg
     2461 tgatattgtc atatatgaat ctaccgtata tcccggatgt acggaagaag tttgtgtgcc
     2521 ggtactggaa catgccagcg ggctgaaata caatgaggat ttcttttgcg gttactcgcc
     2581 tgagcgcatc aatcccggcg ataaggtcaa taccctgaca aagatcaaaa aggtgacctc
     2641 tggttcaaca cctgaggttg cacaggaggt ggatgccttg tatgcttcca tcattgaagc
     2701 aggcacccac ctggcaccat cactcaaagt ggcagaggcc agcaaggcga ttgagaatgc
     2761 ccagcgagac ctgaacatct ctttcatgaa tgagctggca ctcatctttg aaagaattgg
     2821 tatagataca aatgatgtac tggaggcagc aggaaccaaa tggaattttc ttcgcttcaa
     2881 acctggtctg gtaggcggac attgcattgg ggtggatccc tattacctgg ttcataaagc
     2941 tgagtctgta ggttatcatc cccaggtcat cctgtccgga cgcagggtca atgacaacat
     3001 gggcgtctat gtagccaaca ggcttgtaaa gcgcatgatc agcaaagggg tgaccataaa
     3061 aggaaatcgt gtgctgattc tcggtattgc tttcaaggag aattgtccgg atactcgtaa
     3121 taccaaggtc gtagatattt ataatgagct tcgggagttc ggactggaag tcgatgtgta
     3181 tgacccctgg gtagatccga aagaggtcat gcatgagtat ggcatctccc tgttggaaga
     3241 agaacctaag gggccttatg caggaatttt ggtggctgta gcacacaggc agttcgctga
     3301 aatgaacctg tcagccctga aagcaaacga ggccgtggta tttgatacca aggcattttt
     3361 agacaggtcg ctcgttgacg agcgactgta attctatgtc acttaagaaa caggcatttt
     3421 ccggtttcgt ctggaccttt accagcaacc tgggaaccaa gttcaccacc ctggtggtag
     3481 gggtgatact ggcccgctta ttagctccgt cagatttcgg tctcattgcc atgctctact
     3541 tcttttttga agtgtccaga gtactggtgg attcgggatt cagtcaggcc atcatccgtg
     3601 aaaaagagat caacgaagaa gacaaatcaa ctgtctttta catcagcctg tttactgctg
     3661 ttatctgcta tattgctttg tggatcggtg caccggctat cgcacggttc tatgaagaac
     3721 cacaacttac cctgcttaca cgggtgatgg gtcttaacct gatcttcgca tcttttacca
     3781 tcgtacagaa tgcggttttg atcaatgagc tgaagtttaa aactctcgga attatcgcaa
     3841 tggcagccaa tgtgctcacc ggattgatcg cactgtttct cgcatggagt ggtatgggtg
     3901 tcatggctct ggccatcagg tatgtgctaa catcattgat cacgtccatc ctgtattttg
     3961 ctgtgctgac ctggtatcct aaagtgttca tacaaaaacg atccttaaaa aagttattcg
     4021 gatttggttc acgggtattg ctgctgggag tgatgaacac attcttccga aacatctata
     4081 acctgctgat aggcaagatc tatacgccgg ctatcctgag tttctacagc caggccaata
     4141 tgttcaagga gaatgcatct cgcggactga tcgattcact gagcaaggtc acctatccga
     4201 tcctgagcaa ggacagagac aatcgggcaa ggatgaaaga gaactaccgg aagatcctgc
     4261 aggtaaccag ttttgtagtg ttcccctcca tgatcttgct ggcctttgta gctgaaccga
     4321 tgatactggc actggttgga gagaaatgga gacaatccat tccactgttg cagatactct
     4381 gtgtagccgg gctggtatca catatacatg tggtgaatct aaatgtactg aaggtgtatg
     4441 ctgcaggaaa tcaatacgta ttgcagggta tcattaagta tgcccttaca gttgcaggtg
     4501 tgttggtggg tatgcagttt ggattgtcag gtctgatgta tggaattgtt gtagtggaat
     4561 atctgcactt gctggtcaac atctatttga gtaatcagca tctgtcttac acatattccg
     4621 aacagggact tgatctgctg aaagttttct ggctaaacct gccgatggcc ggtgtccttt
     4681 ggctgacctc tttacccgaa tatcctgcac cagtgtttca gcttctggtt atgaccggga
     4741 ccggactggt catgtatata cttactgctc acctgacaaa tgctatggca tggaagtaca
     4801 tggtagaatt gattcagggg caactgagaa aacgcaagat atcaactgca gcagaaacca
     4861 ccgcacatgt ggaaacagct taggcagata tttacaatga accaaactcc gatcctgatc
     4921 ggagggtgcg ggagaacagg taccagtttg atgacggcca ttctggatgc acatccagac
     4981 atttactgta ttccggcata gaccaatatt tttctgacca gacgtgattt caagtctgat
     5041 ttctggaacc accagataca gcgaattcga atcaccagag acttgctgaa acgtgatatt
     5101 cccgcatctt ccaaacgatg gtgtgagaaa tcgcctccaa atgtcaggta cctggagcaa
     5161 ctctttcgag agtttggcag ggatctgaag gttatactta tgatacgtga cggcagagat
     5221 gtgatcacga gtatgcatcc cctccgggaa ggatactata ttcctattga gcgttggatc
     5281 aatgatacca gacaaacgat caggtggaag gatcacccgc aggtcctatt ggtcccatac
     5341 gaatctttgg tgagtaactt ccgcccggtc atcgagcagg tattgacatt tctcgaggct
     5401 gccatgagtc aggaggtgct ggactacacg aactattccg gtgttcagca gcatgatgca
     5461 tttcacgggc aacaccttag accactatat acggcatctc aaagaaaatg ggagcttccg
     5521 gaacacaggg aaagaataga tctgttcctg cagaacgaag aagtacagga gctgatgaat
     5581 agtatcgttg atatcagaca ggcttttgac ctgagtttgg aaaatgtgga acgggcatga
     5641 agatcctgat catttcaaag ttcttttatc cttctgtaac tccacgatca tttcgtacta
     5701 ctgcacttgc taaggcattt gccaaagccg ggcatgaagt acaggtctat acttcctgga
     5761 atgaagaaat tcatcctgca tatgctgttg aacacggcat ggagatacat ccgctatcaa
     5821 aatggatctg gaaagatatc cgtgcagaca agggagcagg cccgttcaga ttggtacggc
     5881 gaatactgaa
//
LOCUS       DAOMAX010000165         5867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2207_length_5867_cov_4.016862, whole genome shotgun sequence.
ACCESSION   DAOMAX010000165 DAOMAX010000000
VERSION     DAOMAX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2207_length_5867_cov_4.016862"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PK742_08895"
     CDS             complement(<1..1475)
                     /locus_tag="PK742_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76745.1"
                     /translation="MNSKLLIVLSVFLAPLMLHAQGTLETEEVDVIRDYNPILADAVK
                     VTINGEVLPASDSVGELTYSMPVVFYQAPYSPIKIKPVALSKTPQDELSLNYLKAGFG
                     TQFTPLGEVYINSNRSDKFQYGLAARYTSSNGSRENQRYSDLDVDGRSKFYIKKEYVI
                     PVNLFYRNDIVHYYGYNEDSISFPKDSVRQRYNRFGFDLGLANLKENDLDVDFNISGG
                     VKGVQDLAGYRQLNPWLDLTADKELKKHHTTGAGLNIDYFDYNGLQPYYNSFTTLALR
                     YNIEEEGWSVHASLGNTVSSDSKYYAVPDVDFSKDLVGDKLVFIAGIDGQLQRNNYYT
                     LTEENPFLADSIEIRPTSRYELYGGIRASTSGNLSFSAKGYYTPFEDMVFFVNQSADS
                     LRFIPLYGDGTMAGVAVELGYFVPDRIGFSAALNAFTFSELSNVAEAWHTPNLTWRLG
                     TDYHFNSKLTAAAELYGLGKMPALLADGTTSELKGTIDVNLS"
     gene            complement(1487..4591)
                     /locus_tag="PK742_08900"
     CDS             complement(1487..4591)
                     /locus_tag="PK742_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76746.1"
                     /translation="MRNQPFNLILQVCLLLTVSIPASGQQTQKRLDPYRTFEQAKELL
                     ENGKYATAQLLFDQAAAYQAPAGKQEIFLYQAESEYYAAICAIELNNPDAENLMLDFV
                     ANYPGHALQGSANFQLGKLYFKKRRYGDAIEWFELVDIYDLGAEDKDTYRFQYGYCLF
                     TKKRLTEAKKYFQPIKDKQGVYFLPANYYYGFIAYFESDYEEALKSFERVKDDPVYSL
                     VVPYYFANIYFSQGKYQEVVDYTEPLIDNTRLNYYNELNQLIGKAYFNLKQYSKALPY
                     LEYYLEKQRKGTDNDYYQVAFCQFKLDQPEQALVYLEKIRGTGDSLAQHADYLEGRCL
                     LDLDKKAEARNAFQEAASKGVDERVREHASFNYGKLSYELGYYPAAVSSLKDFIETYP
                     KSQNYEEAQELLAESLLRTRNYDEALAILEKLDKTNNKLKQAYQKVAYYSGVNAYNDK
                     QYQDAYKLFNKSLEMPIDGGLQAACFFWLGEIDYANEDYASTIRNQNKFIELSKGSVK
                     LPDEVKQEYANYCIGYAYFRQSKFDNASASFEKALSKLKPSSTGGTRNDIALDATLRA
                     ADCYFMLKDYKKADKYYGTVATNKYKGSDYALFQQGMLNGLQGKNESKISILKKLISD
                     YKNSLYVDDAMYEIANTYFLMENSQAAITNFKALLSDKPGNPYTVKAYLKLGLIYYNL
                     NDLKLAEEYYRKAYELSPTTAEGAEAKDALKDIAEETGNTDGLDGVATASEKDSISYN
                     AAMIKYNRQEFANAVKLFNDYLKEFPKGFFRDQAFFYRGESYFRQEQYASALKDYGRI
                     IDENRLQFLESALVRASWIMFYQNKDYSKANDYYTQLYNVASYKENTYVAMKGLLRTS
                     YLLNDYDEVILNAGRILASDMVTTDEQIEAHFYSGKAYLAKGEIDKAYNSFRSTANLT
                     TNETGVESRFIMADILFQKNKLEDSKKQCLEIINDLPAYEEWVIRSYILLADIAAAKG
                     DYAQAKASLRSIIDNYEGDPELVELAQFKLEQVEEMERNGRVIQDNSNDDSDPDELQF
                     NKDN"
     gene            complement(4769..5227)
                     /locus_tag="PK742_08905"
     CDS             complement(4769..5227)
                     /locus_tag="PK742_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76747.1"
                     /translation="MRTCTLILSLLLLVSCNSQEKGSLDNEILSRKIFDTLKSGDLEK
                     YRLLIPGKAEYKRYYDLKKWDQSSFDQAYDDMRRIQAASFTAFYQDFSDWSVAEYANT
                     QQAAAKTENVAEASLITKIRVNGEVRKYTCKAYKLNGRWYAAGDFQWVPQ"
     gene            5263..5760
                     /gene="rnhA"
                     /locus_tag="PK742_08910"
     CDS             5263..5760
                     /gene="rnhA"
                     /locus_tag="PK742_08910"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQU76748.1"
                     /translation="MVKFDAMVELFTDGAAKGNPGPGGYGVILRYGKHEKEISEGFRL
                     TTNNRMELMAVCVGLEALTRPGLEVVVYSDSRYVVDAVDKKWVFGWERKGFAKKKNPD
                     LWARFLKSYRQHKVRFVWIKGHDGHPENERCDQLAVQASYGRQLLTDSGYEEGLNSGM
                     FDDQD"
BASE COUNT         1438 a         1397 c         1358 g         1674 t
ORIGIN      
        1 gacaggttca catcgatcgt tcctttcaat tcactggtgg ttccgtcagc cagcagcgca
       61 ggcatcttgc ccagtccata caattctgct gcagcggtca gcttgctgtt gaagtgatag
      121 tctgtaccca gcctccaggt gaggttgggg gtatgccagg cttctgcaac attactgagc
      181 tcactgaagg tgaatgcatt cagagccgca ctgaaaccaa ttctgtcagg tacgaaataa
      241 cccagttcca ctgccacacc tgccatagtt ccatcgccgt ataaggggat gaagcgaaga
      301 ctatccgccg actggttaac gaaaaagacc atgtcttcaa acggagtata ataccctttg
      361 gctgagaatg acagatttcc gctggtagag gccctgatac ctccatataa ctcatatctg
      421 gaagtaggtc tgatctcaat actgtcggca aggaatgggt tctcttcggt cagcgtatag
      481 tagttattgc gttgcagctg cccgtctata ccggcaatga ataccagctt atctcctacc
      541 agatccttac tgaaatctac gtctggtaca gcatagtatt tactgtcaga gctcaccgtg
      601 ttgccaagag aagcgtgcac actccagcct tcttcctcaa tattatacct gagagcaagc
      661 gtggtgaatg aattataata aggttgtaac ccgttgtaat caaaatagtc gatgtttaaa
      721 ccggcccctg tggtatggtg tttcttcagt tctttatcgg ctgtcagatc cagccaagga
      781 ttcagctgtc tgtatcctgc caggtcctgc actcctttta ctccacctga aatattaaaa
      841 tctacatcca ggtcattctc tttcaggttg gccagcccca gatcaaaacc gaacctgtta
      901 taccgctgcc tgacactgtc tttcgggaag cttatgctgt cttcgttgta tccgtaatag
      961 tgaacaatat cattcctgta gaacaggttt acaggaatga catattcttt tttgatatag
     1021 aacttacttc ttccatctac atcaagatcg ctgtagcgct gattctctct ggaaccgttg
     1081 ctgctggtat atctagcagc cagtccgtac tggaacttat cagacctgtt ggagttgata
     1141 tacacctctc ccagtggtgt gaactgggtt ccgaaaccgg ctttcagata gttgagtgac
     1201 agttcatcct gcggagtttt actcaatgct accggcttga tcttgatggg agaataggga
     1261 gcctgataaa aaaccaccgg catactatag gtcagttcac caacagagtc agatgcaggc
     1321 agcacttctc cgttgatggt caccttgacg gcatcagcca gaatgggatt gtagtctctg
     1381 atcacatcta cctcttctgt ttccagtgta ccttgtgcat gcagcatcaa aggcgccagg
     1441 aatacagata atacgatgag caacttgctg ttcatggatg ctgttttcag ttgtctttgt
     1501 tgaattgtag ttcgtccgga tcactgtcat cattactgtt gtcttgtatg acgcgtccgt
     1561 tgcgttccat ctcctctacc tgttccagtt tgaactgtgc cagttccacc agttccggat
     1621 cgccttcata attatcaatg atactgcgca acgatgcttt ggcctgggca tagtcgcctt
     1681 tggcggctgc tatatccgcc agcaggatat aagaacggat cacccattct tcataagcag
     1741 gcaggtcatt gatgatctcc agacattgct tcttggagtc ttccagtttg ttcttctgga
     1801 ataggatgtc ggccatgatg aacctggatt ccactccggt ctcattggta gtgaggttgg
     1861 ctgtggacct gaacgaattg tatgccttat cgatctcgcc ttttgccagg taggccttac
     1921 cactgtaaaa atgtgcttct atctgttcat cagtggtcac catatcgctg gccaggatgc
     1981 gaccggcgtt caggatgacc tcatcataat cattcagcag gtacgaagtg cgcagcaagc
     2041 ctttcatggc cacataggta ttctccttgt agctggctac attatacagc tgcgtatagt
     2101 aatcgttcgc cttgctgtag tctttgttct gatagaacat gatccagctg gcacgaacca
     2161 gcgcagactc cagaaactgt aacctgtttt cgtcaatgat ccggccgtag tccttcagtg
     2221 ctgaggcata ctgttcctgg cggaagtaac tctcaccgcg atagaagaat gcctggtcgc
     2281 ggaagaaccc tttcgggaat tccttcagat agtcattgaa cagcttgacc gcattggcaa
     2341 actcctgtct gttgtatttg atcattgccg cattgtagct gatgctgtct ttttcagacg
     2401 ccgtggccac gccatccaga ccatcagtgt ttccggtctc ttctgcgata tctttcagtg
     2461 catcttttgc ttcggcaccc tctgctgttg taggcgacag ttcatacgct ttgcggtagt
     2521 attcctccgc cagcttcagg tcgttgaggt tgtaatagat caatcccagt ttgagatatg
     2581 ccttgacggt ataagggtta ccgggtttat cgctgagcaa cgccttgaag ttggtaatag
     2641 cagcctgcga attttccatc aggaaatagg tattcgcaat ctcatacatg gcgtcatcca
     2701 catagagtga attcttatag tcgctgatca gcttcttcag aatagagatc ttcgattcgt
     2761 tcttaccctg caatccgttc agcatacctt gctgaaaaag tgcatagtct gaccctttgt
     2821 atttattggt ggctacggtg ccgtaatact tatcagcttt tttgtaatct ttcaacatga
     2881 aataacaatc agcggcacga agggtggcat ccagggcaat atcatttctg gttccaccgg
     2941 tggatgaagg cttgagtttg ctgagtgcct tctcaaagga agcagaagca ttatcaaatt
     3001 tagactgccg gaaataggca tagcctatac aatagttggc atattcttgt tttacctcat
     3061 caggcagctt tacactgcct ttagacagtt ctatgaattt gttctggttg cgtatggtgc
     3121 tggcatagtc ttcatttgcg tagtcgatct ctcccagcca gaaaaaacag gcagcctgta
     3181 aaccaccgtc aattggcatc tccaacgatt tattgaacaa cttgtaggca tcctgatact
     3241 gcttgtcatt gtaggcattg acaccactgt aataggctac tttctgataa gcctgtttga
     3301 gcttgttatt ggtcttgtcc agtttctcca gaatggccag ggcttcatca taattcctgg
     3361 tgcgcagcaa tgactcagcc agtaattcct gggcttcctc atagttttga gatttcgggt
     3421 aggtctcaat gaaatcttta agtgaactga ctgctgccgg ataataaccc agctcataac
     3481 tcagctttcc gtaattaaag ctggcatgtt ccctgaccct ttcatctact cctttggaag
     3541 ccgcttcctg aaaagcattc cgggcttccg cttttttatc cagatcaaga aggcacctgc
     3601 cttccagata atcagcgtgt tgagccagag aatctccggt accacgaatc ttttccagat
     3661 ataccagagc ctgttcaggc tggtccagtt tgaactggca aaaggccacc tgatagtaat
     3721 cgttatctgt gccctttctc tgtttttcga gataatattc caggtatggc agtgctttgc
     3781 tgtattgttt cagattgaaa taagccttac ctatcagttg gttcagctcg ttgtaatagt
     3841 tcagcctggt attgtctatc aggggctcgg tatagtcaac cacctcctga tatttgccct
     3901 gagagaaata gatgttggca aaataatacg gcactacaag agaataaaca ggatcatcct
     3961 tcacccgttc aaagctttta agtgcttcct cataatcact ttcaaaatat gcgatgaaac
     4021 cgtaataata attggcaggt aaaaaataca ctccctgttt atccttgata ggctggaaat
     4081 atttcttggc ctcggtaagt cgctttttag tgaacaggca atagccatac tgaaagcggt
     4141 aagtgtcttt atcttccgca cccagatcat agatatccac cagctcaaac cactctattg
     4201 catctccata gcgtctcttc ttgaagtaca gtttacccag ttgaaagttg gctgacccct
     4261 gaagggcatg cccaggatag ttggccacaa agtccagcat cagattctct gcatcggggt
     4321 tgttcagctc aatggcacag atggccgcat aatattcgct ttcggcctgg tacagaaaga
     4381 tctcctgttt tcctgccggt gcctgataag ctgctgcctg atcgaacaac aattgcgcag
     4441 tggcgtactt tccgttctcc agcagttctt tggcctgctc gaaggtccgg taaggatcca
     4501 gtcgtttctg ggtctgctga cctgatgccg gtatgctgac agtaagcagc agacacacct
     4561 gtaggatcag gttgaatggt tggtttctca tacgtctgta aaaggtaaag cttctttgat
     4621 agcgtgctga ttagtacaaa accagaatcg tgccgaagtt aagaaggatc ataggcgggc
     4681 tgtggctgtg ctgttaaaat tgaatacaca actgcttgtt gataatcaac gacaaagggc
     4741 gggaaaatat tggatgccgg gtgcggtatt actgtggcac ccactgaaaa tcgccggcag
     4801 cataccatct tccgttcagc ttgtatgctt tgcaggtgta cttgcgcacc tctccgttca
     4861 cacgtatctt ggtgatgaga ctggcctccg ctacattctc tgtctttgca gctgcctgct
     4921 gggtattggc atactctgca acagaccagt ctgagaaatc ctgatagaat gctgtgaacg
     4981 aagctgcctg aattctgcgc atatcatcgt atgcctgatc aaaagatgac tggtcccatt
     5041 ttttcagatc atagtagcgc ttgtattctg cttttccggg aatgagcagg cggtatttct
     5101 ccagatcacc tgacttcagg gtatcgaaga tcttcctgga aagtatctcg ttatccaggc
     5161 tgcccttttc ctgactgtta caactaacaa gcagcaacag actcagtatc agtgtacagg
     5221 ttctcataca gacagggtaa atgtacttca aaatccaaat taatggtcaa atttgacgcc
     5281 atggtagaat tgttcactga cggggcggca aagggaaacc cggggccggg agggtatggt
     5341 gtaatcctgc gttacggaaa gcacgaaaaa gagatcagtg aaggattccg gcttaccacc
     5401 aataaccgca tggaactgat ggccgtatgc gtggggctgg aagccctgac ccgtccggga
     5461 ctggaagtag tggtgtacag cgattccagg tatgtagtgg atgcggtcga caagaaatgg
     5521 gtgttcggct gggagcgtaa gggttttgcc aagaaaaaga acccagacct ttgggccaga
     5581 tttctaaaaa gttacagaca gcataaagta cgctttgtct ggatcaaagg ccacgacggg
     5641 caccctgaaa atgaacgctg tgatcagtta gcagtacagg catcttatgg cagacaactg
     5701 ttgaccgatt caggctacga agagggtctg aacagcggga tgtttgatga tcaggactga
     5761 gcaaggccgg cagactcacg gctggcttcc agaaactttt ctacatcttc caccatcaac
     5821 ctggaaccta tatacaacgg gcagcgctga tgcagctctt tgggatc
//
LOCUS       DAOMAX010000166         5843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2220_length_5843_cov_3.966655, whole genome shotgun sequence.
ACCESSION   DAOMAX010000166 DAOMAX010000000
VERSION     DAOMAX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2220_length_5843_cov_3.966655"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1036
                     /locus_tag="PK742_08915"
     CDS             206..1036
                     /locus_tag="PK742_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76749.1"
                     /translation="MRLIRILTFSNFWIAVGAAAFCQAGYLFLGDQPHWDLLNLFCFL
                     SCWSVYLLIRVAAVKRITHYERTHRWQFFLDHIRFFRLLTFSGLLAVAVIYFFLPFRV
                     RMVLLLPGFISLIYGLPVTARRRKLRDIGIIKVFLIGFVWAFVGSILPAAAGEGAFSP
                     DTIALFFAQFLFIFANALPFDISDMKSDAMHAVRTIPYYLGAELTYTLSFVCLVASGA
                     IHGYFLPDTAVPAGVTIMLSGLLIYMSGQIKHEQVLFFGIDSLMLLQFLLVWSYLFVH
                     "
     gene            complement(1037..1444)
                     /locus_tag="PK742_08920"
     CDS             complement(1037..1444)
                     /locus_tag="PK742_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76750.1"
                     /translation="MAIMHHIDRKQLWFLLIVGALFFAGTWMRRDKQDQTPTTEMLDA
                     QLLKDLQGRTLQADEHTLCIMECAGITTTDLKDLLVSENLDQDQCDFGNCHYTSYALK
                     GHLKNGKQVSFTVETGDGGDILKDLQLPDDCPC"
     gene            1499..2485
                     /gene="bioB"
                     /locus_tag="PK742_08925"
     CDS             1499..2485
                     /gene="bioB"
                     /locus_tag="PK742_08925"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HQU76751.1"
                     /translation="MAEIRNDWTRDEIAEIYNGPIMELMYKASVVHHEMQATGEVQVC
                     TLLSVKTGGCPEDCAYCPQAARYQTEVQVHKLLEYDEVITKALIAKENGSTRFCMGAA
                     WRNIRDNRDFDNVLEMVKGVSGLGLEVCCTLGMLTEDQARKLKEAGLYAYNHNLDTSE
                     EHYHDIITTREYKDRLDTLTHVRNAGVSVCSGGIIGLGESEEDRVGLLHTLATLPQHP
                     ESVPVNALVPVKGTPLEEMKRVSVWEMIRMIATARIIMPGSMIRLSAGRDEMSVEEQA
                     LCFMAGANSIFAGEKLLTTPNPEFHEDKAMFELLGLTPRPSYKQHPSLAESN"
     gene            2488..3603
                     /locus_tag="PK742_08930"
     CDS             2488..3603
                     /locus_tag="PK742_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HQU76752.1"
                     /translation="MQELPERLRQKLADRKTAHALRALRQFPALIDFSSNDYLGFSGN
                     IRIANAVNNYLLSGGETGSGGSRLLTGNHPKLVEAEEYIAKFHHAEAALFFGSGYEAN
                     SGLIATVCHRHDTIIHDELCHASLREGIRASGAKAISFAHNDLHDLQDKLQHVTGNKW
                     VITESVFSMDGDICPLLDLVTISRSNGAMIILDEAHATGVIGKKGEGLAQSCQVQDQI
                     FARVHTFGKAIGVQGAVVMGSRELRSALVNFCRPFIYTTAPSPMLAVAAAAAYSLLDG
                     PCDEWQKLQELIHFADELQDVIKGEGTAIRQVMIPGNEAVRSAAEQIQHAGFLVLPVL
                     SPTVPAGSERIRICLHAYNTKEELTALTELIKTIRQQ"
     gene            3600..5147
                     /locus_tag="PK742_08935"
     CDS             3600..5147
                     /locus_tag="PK742_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQU76753.1"
                     /translation="MIREVQLRLTPEQAQDPDLLRKEAISKAGAGASAKVELVRRSID
                     ARQKQVMLQLVVRVYEEEEMPDNAFALPDLSSVTDAPEVHIIGCGPAGLFAALTCLQR
                     GLKPVILERGKDVRSRRHDLAAINRRHIVDPDSNYCFGEGGAGTYSDGKLYTRSGKRG
                     DIRKVLEWLVAFGASHEILVEAHPHIGTNKLPGIITAIRQFILDHGGEVHFGKRVTAI
                     QTKNGKVSQLECNGQENIPVAQVILATGHSARDIFFMLHSSGIPVEFKPFAMGVRIEH
                     PQVLIDQMQYHCDVRSAYLPPASYSMVEQVNKRGVYSFCMCPGGIIAPCATASEEVVT
                     NGWSPSKRNNPFANSGFVVSVEEKDAEAYAHHGPLKGLAFQMATEYNAWQYGGYTQAA
                     PAQRMVDFVQKKATTELPACSYQPGVVSARMSEVLPDFIANGLRGAFLQISKHKPAYY
                     TNEAILVGVESRTSSPVRIPRDPDTLQHPGVSGLFPCGEGGGYAGGIVSAAMDGIKVA
                     MAIPAGV"
     gene            complement(5155..5541)
                     /locus_tag="PK742_08940"
     CDS             complement(5155..5541)
                     /locus_tag="PK742_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU76754.1"
                     /translation="MKKPATVDAYLKEFPDPVKSRMEQIRAAIKKAAPDAEEMISYGM
                     PAYKTFARPLVYYAGYEHHIGLYATPTGQLAFAKELAGYKQGKGSVQFPNDAPFPLPL
                     IKKIIAFRVAENRDKYAPKATKKITK"
     gene            complement(5538..>5843)
                     /locus_tag="PK742_08945"
     CDS             complement(5538..>5843)
                     /locus_tag="PK742_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76755.1"
                     /translation="EVLGFTCGARNNDWGWAALHLNDVEIMLAIPNAHTPFDAPKFTG
                     SIYINTDDATQFWEQVKDKARICYAPEDFEWGMREFGIFDNNGYLLQFGQDLIMESP"
BASE COUNT         1452 a         1380 c         1506 g         1505 t
ORIGIN      
        1 ttgggttggc cgggcataaa actcatcgcg attggcaagc acgaccaatg ggtaaatatc
       61 atgagtttgc caggcaatat tgatcagaca catggatcag aagatggtgt gttggcaata
      121 aacatacaaa acgaaacgat gcagttggta gttcggttca ttatccggaa tctccttcct
      181 gcaggctatt ccttaccttt atccgatgcg cctgattcgc atactcactt tctccaattt
      241 ctggatagct gtgggcgcag cagcattttg tcaggcaggc tatttgtttc tgggtgacca
      301 gccacattgg gatctgctta atttattctg cttcctttcc tgctggtctg tgtacctgtt
      361 gatcagggtg gctgcggtaa aacgaattac gcattatgaa cgtacccatc gctggcagtt
      421 ttttctggat catatcaggt tttttcgtct gcttactttt tccggtttgc tggccgtagc
      481 tgttatatat tttttcctgc ctttcagggt gcgcatggtt ttattgcttc cgggttttat
      541 ctctctcatc tatggcctgc cggtcactgc ccgaagaaga aaactccggg atatcggtat
      601 catcaaagtc tttctgatag gttttgtctg ggcttttgtg ggaagcatcc ttcctgctgc
      661 agctggagag ggcgcattca gcccggatac aattgcttta ttctttgcac agttcctgtt
      721 catttttgcc aatgcactgc catttgatat cagcgatatg aaatcagatg ccatgcatgc
      781 agtgcgcact attccctact atctgggcgc agagctgacc tacaccttgt cttttgtttg
      841 tctggttgca tccggagcca ttcatggtta tttccttcct gatactgccg ttccggcggg
      901 ggttaccatc atgctgagcg ggcttcttat atatatgtcg ggacagatca aacacgaaca
      961 ggtcctgttc tttggaattg acagcctgat gctgcttcag tttctactgg tatggagcta
     1021 tttgtttgtg cactgatcag caggggcaat cgtccggcaa ctgcaggtct ttgagaatat
     1081 cgccaccatc tcctgtttct actgtaaagg acacctgctt cccattcttt agatgtcctt
     1141 taagagcata actggtataa tggcagttgc caaaatcaca ttgatcctga tccagatttt
     1201 cagagaccag cagatccttc agatctgtgg tcgttatgcc tgcacattcc atgatgcaca
     1261 aggtatgctc atcggcctgc aaagtacgtc cctgcagatc cttcagcaac tgtgcatcca
     1321 gcatttcagt ggttggtgtt tgatcctgtt tgtctctgcg catccaggtg ccggcaaaaa
     1381 agagagcacc cactatcagt aagaaccaca gttgtttacg atctatgtga tgcataatgg
     1441 ccatgacaac gatttgtgca aaattacctt tcggttacct ttgccacaaa ccttccacat
     1501 ggcagagatc aggaatgact ggacccggga tgagatcgct gagatctaca acgggcctat
     1561 catggagttg atgtacaaag cttcggtagt gcaccatgag atgcaggcta ccggcgaggt
     1621 acaggtttgc accctcctca gtgtaaagac cggaggttgc ccggaagact gcgcttattg
     1681 cccgcaggca gcccgctatc aaacggaggt gcaggttcac aagttgctgg aatatgatga
     1741 ggtcattacc aaggccctga tagcgaaaga gaacggcagc acacgatttt gcatgggcgc
     1801 tgcgtggaga aatatcagag acaaccgtga ttttgataat gtgcttgaaa tggtgaaagg
     1861 agtttccggg cttgggctgg aagtgtgttg cacactgggc atgctcacag aagaccaggc
     1921 tagaaaactc aaggaagcag ggctctatgc ctataaccat aatctggata cttctgaaga
     1981 gcattaccac gacatcatca ctaccaggga atacaaggac aggttggata cactgaccca
     2041 tgttcgaaac gccggggttt ctgtatgttc cggcggcatc atcggactgg gtgaaagtga
     2101 ggaagaccgg gtaggcttac tgcatacact ggccaccctg ccacagcacc cggaaagtgt
     2161 tcctgtcaat gcgctggtac ctgtgaaagg aacacccctg gaagagatga aaagggtaag
     2221 cgtatgggaa atgatccgga tgatcgctac cgcccgcatc atcatgccgg gaagtatgat
     2281 caggctcagc gcaggaaggg atgaaatgtc ggtggaagaa caggctttgt gcttcatggc
     2341 aggcgccaac agtattttcg ccggcgagaa gctcctgacc acacctaacc cggagtttca
     2401 tgaagacaaa gccatgttcg aattgctggg attaaccccc aggccttcct ataagcagca
     2461 tccatcactg gcagaatcca actgatcatg caggaattgc cggaacgact gcggcaaaag
     2521 ctggcagaca gaaaaactgc ccatgcactg agagcactca ggcaattccc ggcacttatt
     2581 gatttcagtt ccaacgatta tctgggcttc tccggcaata tccgcattgc aaatgcagtg
     2641 aacaattatt tgttgtcagg cggtgaaacg ggaagcggcg gttctcgcct tcttacaggt
     2701 aaccacccga agctggtgga agcagaagag tacatagcaa aatttcacca tgccgaagca
     2761 gcgctttttt ttggttccgg ctatgaagcc aacagcggac tgatcgcaac tgtttgtcac
     2821 cgacatgaca ccataataca tgatgaatta tgtcatgcaa gtcttcggga aggcatccgg
     2881 gcatcaggtg cgaaagcgat atcatttgca cacaatgatc tgcatgatct gcaggataag
     2941 ctacagcatg taaccggcaa caaatgggtc attacagaat cagtgttttc tatggacggc
     3001 gatatttgtc cgctgctgga tctggtaacc atctcccggt caaatggtgc catgatcatc
     3061 ctggatgaag cacatgcaac aggggtgatc ggaaagaaag gcgaaggact tgcccaatca
     3121 tgtcaggttc aggatcagat attcgccagg gtacatacat tcggaaaagc aattggagtg
     3181 cagggtgctg tggtcatggg aagcagggaa ctgagatctg cactggtaaa cttctgtcgt
     3241 ccgttcatct acactactgc accttcaccg atgctggcag tggctgctgc tgctgcctat
     3301 tctttgttgg atggtccatg tgatgaatgg cagaagttac aggaactgat acattttgca
     3361 gatgaactgc aggatgtcat caagggggaa ggcaccgcca tcaggcaggt gatgattccc
     3421 ggcaatgaag cagtcaggtc tgctgctgag cagattcaac atgcaggttt tctcgtcctt
     3481 cccgtattat ctcctaccgt accggccggc agtgaacgga ttcgcatctg tctgcatgca
     3541 tacaatacta aagaagagtt gacggctctt actgaattaa tcaaaacgat caggcagcaa
     3601 tgatcagaga agtacagctt cggctaacac ccgaacaggc acaggatccg gatctgctga
     3661 gaaaggaagc catcagcaaa gccggagcag gagcatctgc caaagtggag ttggtcaggc
     3721 gcagtataga cgcacgccag aaacaggtaa tgctgcaact ggtggtccgt gtttatgaag
     3781 aagaagagat gccggataac gcctttgcat tacctgatct ttcatcggtg actgatgcac
     3841 cggaggtgca catcattggt tgcggtcctg caggtctttt tgcagcactg acctgtttgc
     3901 aaagaggact gaaacctgtc attctcgaaa gagggaaaga tgtgcgcagc agaaggcatg
     3961 atctggcggc gatcaatcgc aggcacatag ttgatcctga ttcaaattat tgttttggcg
     4021 aaggtggtgc aggtacctac tctgacggaa aactttacac ccgttcaggc aagcgtggcg
     4081 acatccgcaa ggtactggag tggctggtag catttggagc ttcacatgag atactggtag
     4141 aagcgcatcc tcacatcggt accaataaat tgccgggtat cataacagca atacgacagt
     4201 tcatcctgga ccatggcggg gaggtgcatt ttggcaaaag ggtcactgcc attcagacaa
     4261 agaatggaaa ggtgagccaa ctggaatgca acggacagga aaatattcct gttgcacagg
     4321 tcatactggc aacaggacat tctgcccgtg atattttctt catgttgcac agctctggca
     4381 ttcctgttga attcaaacca tttgccatgg gtgttcgcat agagcacccg caggtattga
     4441 tcgatcagat gcaataccat tgcgatgtgc gttcagcata tctgccgcct gcatcctaca
     4501 gcatggtgga acaggtaaat aaacgtggcg tgtacagttt ctgtatgtgt ccgggaggca
     4561 tcattgctcc atgcgctact gcatctgaag aagtggtaac caacggctgg tcaccgtcta
     4621 agcggaataa cccgtttgcc aatagtggat ttgtggtatc tgttgaagaa aaagatgcag
     4681 aggcgtacgc acaccatggt ccgttgaaag gactggcatt tcagatggcc acagaataca
     4741 atgcctggca atacggagga tacacacagg cagctccggc acagcgcatg gtggattttg
     4801 tacagaagaa agcaacaaca gagcttcctg cctgcagcta ccagcccgga gttgtatctg
     4861 ccaggatgag tgaagtgttg cccgatttta tcgccaacgg attacgcggt gcattcctgc
     4921 agatcagcaa acataagcct gcctattata cgaatgaagc catcctggtg ggggtggagt
     4981 ccagaacctc ttcaccggtg cgcataccca gagatcccga taccctgcaa cacccgggtg
     5041 tgtcgggtct gtttccctgc ggggagggag gaggctacgc aggaggaata gtcagtgcag
     5101 cgatggatgg gatcaaagtg gcgatggcta ttccggccgg ggtttgaatg cgcatcactt
     5161 tgtaattttt tttgttgctt ttggagcgta cttatcccgg ttctcagcta ccctgaaagc
     5221 tatgatcttt ttaataagtg gcaatggaaa cggggcatca ttcggaaact gaacggatcc
     5281 ctttccctgc ttgtatcctg ccagttcttt ggcaaatgcc aactgtccgg taggggttgc
     5341 ataaagtcct atatgatgct cataaccagc ataatatacc agcggccttg caaaggtttt
     5401 gtaagccggc atgccatagc tgatcatttc ttcggcatca ggagcagctt tttttatggc
     5461 cgctctgatc tgttccatgc ggctctttac cggatccgga aattccttca ggtaggcatc
     5521 aactgttgca ggttttttca tggactttcc ataatcaggt cctgcccgaa ttggagcagg
     5581 tatccgttgt tatcaaaaat gccgaactcc cgcatgcccc attcgaaatc ttccggcgca
     5641 taacatatcc gggccttgtc ttttacttgc tcccagaatt gggtagcatc atccgtgttg
     5701 atgtagatgg atccggtgaa tttcggtgcg tcgaatggtg tgtgtgcgtt cggaatggcc
     5761 agcatgatct ccacgtcatt caggtgcaat gctgcccatc cccaatcatt atttctggcc
     5821 ccgcaggtga atcccagcac ttc
//
LOCUS       DAOMAX010000167         5814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2243_length_5814_cov_4.032818, whole genome shotgun sequence.
ACCESSION   DAOMAX010000167 DAOMAX010000000
VERSION     DAOMAX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2243_length_5814_cov_4.032818"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..858)
                     /locus_tag="PK742_08950"
     CDS             complement(136..858)
                     /locus_tag="PK742_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76756.1"
                     /translation="MFSLIFQVFQVMLEFPTIQIGGLTIHEPMTAFTDVILTIISFVL
                     VGRIRNRLHESFYNNAWRLFFLFMGLSTGLGVLTHGLVSLFSAWEHYLLWMAMNVAAS
                     ISVYFALQATIRYSRVSIRNRRLLKRINLSVLVFFLGLTFWQNNFEIFKIHAATGVLI
                     IFLTYFTAWTRNLVGSGAIVLAFLLSIATVFVHSFQLSINEWFNYKDLSHVMMMVSLL
                     LVYHGMQLSSRDLKLNWRRAIR"
     gene            complement(962..1034)
                     /locus_tag="PK742_08955"
     tRNA            complement(962..1034)
                     /locus_tag="PK742_08955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(999..1001),aa:Gly,seq:ccc)
     assembly_gap    1147..1246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1330..2562)
                     /locus_tag="PK742_08960"
     CDS             complement(1330..2562)
                     /locus_tag="PK742_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQU76757.1"
                     /translation="MPGISHRGQQMPASPIRKLVPYAEQAKIRGRKVYHLNIGQPDIE
                     TPPVMLEAVRNMDLHVVEYSHSAGFESYRRKLVDYYKGVGIELNHHQIMITTGGSEAI
                     LFGMFSCLDAGDEVIIPEPLYANYIGFAEAGNIVVKPITASIDNGYALPPIDAFRQQI
                     TDKTKAILICNPNNPTGYLYSKEELEQLRQIVLEHDLFLFADEVYREFVYDGAEHHSV
                     LNQEGLDEHVVLMDSISKRYSACGARIGALVTRNAKVMETALKFAQARLSPPTIEQIL
                     AEAFTGVSDDYFIGVNEEYVSRRDLCVERLNDMDGVRCPNPGGAFYAMAELPVDDTDQ
                     FCQWLLESFDLNGETVMLAPASGFYSTPGLGKQEVRIAYVLNKDDINKAMDCLEAALK
                     VYPGRVSSVGSGQSAVEQ"
     gene            complement(2628..3080)
                     /locus_tag="PK742_08965"
     CDS             complement(2628..3080)
                     /locus_tag="PK742_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQU76758.1"
                     /translation="MKKLFSALAFIAFATAPLFAQETMEEVNPNAPAFEWAETTHDFG
                     TIPQGVPVTNKFSFTNTGKEPLIISNVQKTCGCTVTDWTKEPIMPGQKGFVAAEYNAA
                     KEGPFTKAITVQSNANTPNVKLYFKGTVEKAEQTGSVPEENTIFNSNN"
     gene            3239..5638
                     /locus_tag="PK742_08970"
     CDS             3239..5638
                     /locus_tag="PK742_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQU76759.1"
                     /translation="MSSNVQSDNLSYTEFKQQVLADYRLACLSREASLLGRKEVLTGK
                     AKFGIFGDGKEIAQLAMARVFEDGDFRSGYYRDQTFMFAAGMATIEQFFAQLYAIPDV
                     EEDPFSAGRQMNAHFATRSLDAQGNWKELRSMKNSSSDTSPTASQMVRALGLALASKK
                     YRDVPALNDTPFSFSGNEVVFATIGDASTSEGVFWETVNAAGVLKVPLAFFVWDDGYG
                     ISVPREYQTTKDSISEALSGMQLDEDGNGIRIYEVKAWDYPSLIQTFEDGISFCRENH
                     IPVFFHVKEVTQPQGHSTSGSHERYKSPERLDWEAEYDGIKKMREWMSAHGLASEEEM
                     DQIAEEAKKEVREIQKSAWNKHLMSIKADKDVVIKLLSDLASTGHEQVAAVQKQLETA
                     MDPIRRDVMRAARQALYATAGQDSAQRQALKQWVDDYAAVNHDRYNSYLFSQSSQAVT
                     NISVVPAVYGDDAPLKNGFEILNTCFEENFKRYPGLFAFGEDLGKIGDVNQGFAGLQE
                     KFGEDRIFDVGIREATIIGQGIGMAMRGLRPIAEIQYLDYLIYGLQPISDDLATLQYR
                     TKGGQKAPVIIRTRGHRLEGIWHTGSPMAMILGTCRGVHLCVPRNMVQAAGMYNLLLQ
                     SDEPAIVVECLNGYRLKEKMPDNIADFTVPLGVPEIMRYGDDITVVSYGSTLRVVEEA
                     CNDLEKLGMSIELIDAQTLLPFDIYGVIAQSIRKTNRVIFIDEDVPGGATAYMLDQVF
                     KDKELFQYLDALPQTLSAQPNRSAYASDGDYFCKPNAEDIYEAIYKMMSDVDPDSYPE
                     I"
BASE COUNT         1421 a         1511 c         1467 g         1315 t
ORIGIN      
        1 agagacgaga ttcgaactcg cgaccccaac cttggcaagg ttgtgctcta ccagctgagc
       61 tactctcgca agacctgttt gaaacaggac tgcaaatata caccgtttcc gaaatttcaa
      121 aaatggtacg gtaaattatc tgatggcccg acgccagttc agtttcaggt cgcggctgct
      181 gagctgcatt ccgtgataga ccagcagcag actcaccatc atcatcacat ggctgagatc
      241 cttgtagttg aaccattcgt tgatgctcaa ctggaaagaa tggacgaaaa cagtagcgat
      301 agacagcagg aaggcaagta cgatggctcc gctgcccacc aggttgcggg tccaggcagt
      361 aaaataagtt aggaagatga tcaatacccc ggtggctgcg tggatcttga agatctcaaa
      421 attattttgc cagaaggtga gccccaggaa gaagaccagt acactcagat tgatccgctt
      481 gagcagcctg cggttgcgga tgctgacacg gctatatctg atggttgcct gcagggcaaa
      541 atataccgat atactggctg ccacattcat ggccatccag agtaagtaat gctcccatgc
      601 tgagaacagg ctgaccaggc catgcgtaag tacaccaagc cctgtgctca ggcccatgaa
      661 cagaaaaaac aatctccagg cattgttgta aaaactttca tgcaatcgat tcctgatacg
      721 acccaccagc acaaaggaaa tgatggtcag aatgacatcc gtgaaggcgg tcatcggttc
      781 atggatggtg agtccgccga tctgaatggt aggaaattcc agcattacct gaaagacctg
      841 aaaaatcagg gagaacaaag atgcgttatt tttaagaaat aaaggtcagc agaaggtcag
      901 gagaaccctg gcaaaagtgc ctcattccat acagcgtcag cctcgaaaac cgctgcagat
      961 gagcggcaaa ccggtctcga accggcgacc ttcagtttgg gaaactgatg ctctaccaac
     1021 tgagctactg ccgcttatac aggaaaggta ccagtgaatt ctgttatcca gaataaatgc
     1081 cggaaaaata attgtggaac cttcttcaat cactgctcgc tgcttactgc caactgcttc
     1141 actgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgt ggaaccttct
     1261 tcaatcactg ctcgctgctt actgccaact gcttcactgc tcactgctta ctgctcactg
     1321 ctgtactgct cactgctcta ctgcagactg cccactgcca actgaagaaa cccttcccgg
     1381 atacaccttc aatgccgctt ccaggcaatc catagccttg ttgatatcat ccttgttgag
     1441 cacataggcg atgcgaacct cctgcttgcc cagccccgga gtgctgtaaa agccactggc
     1501 aggggccagc atgacggtct cgccgttcag gtcgaaagac tccagcagcc actgacagaa
     1561 ctggtcggta tcatccacgg gcaattcggc catcgcatag aaggcaccac ccggattggg
     1621 acaacgaaca ccatccatgt cattcagccg ttccacacag aggtcacggc gtgatacgta
     1681 ttcttcgttc acaccgatga aataatcgtc gctcacaccg gtaaatgctt cggccaggat
     1741 ctgctctatg gtgggcggac tcaacctcgc ctgggcgaac ttgagtgccg tctccatcac
     1801 cttggcattg cgggtcacca gggcaccgat ccgggcaccg caggcactgt atcgcttgga
     1861 gatggaatcc atcagcacca cgtgctcgtc cagcccttcc tggttgagta cggaatggtg
     1921 ttcggcaccg tcgtacacga attcacgata tacttcatcg gcaaagagaa agaggtcatg
     1981 ctccagcacg atctgccgta actgctccag ctcctctttg gaatacaggt aacctgtcgg
     2041 gttattggga ttgcagatga gaatggcctt ggtcttatct gtgatctgct gccggaacgc
     2101 atcgatggga ggcagggcat agccattgtc tatcgaagca gtgatcggtt tcaccacgat
     2161 attccctgct tcggcaaagc cgatataatt ggcatataaa ggttcgggta tgatgacctc
     2221 atcgcccgca tccagacagg agaacatacc gaacagaatg gcttcggatc cgccagtggt
     2281 gatcatgatc tggtggtgat tcagctcgat gcctacgcct ttatagtagt ccaccagctt
     2341 tcgccgatag ctttcaaatc ctgccgaatg gctgtattcc accacgtgca ggtccatgtt
     2401 tctgactgct tccagcatga caggcggcgt ctcaatatcc ggctgaccaa tattcaggtg
     2461 gtaaactttt ctgcccctga tcttcgcctg ttcggcatag ggcaccagct tgcgtatagg
     2521 agaagcgggc atctgctgtc cgcgatgaga tattccgggc atggctcaaa aatacaaaag
     2581 gtccgcagga aactcctgcg gacctttctt catgtatgct gattcgatca gttgttgctg
     2641 ttgaagatcg tgttctcttc cggaacactg ccggtctgct cagccttctc aaccgtaccc
     2701 ttgaagtaca gttttacgtt gggcgtattg gcattgctct gcaccgtgat ggccttggtg
     2761 aaaggacctt ctttggcagc attgtattca gcagctacga aacctttctg gccgggcatg
     2821 atgggctcct tggtccagtc ggtcacagta caaccacagg ttttctgtac gttgctgatg
     2881 atcaagggct ctttaccggt gttggtaaaa gagaatttgt tggtcaccgg aactccctga
     2941 ggaatggtgc cgaagtcatg ggtggtctca gcccactcga atgcaggtgc gttcgggttg
     3001 acctcttcca tggtttcctg cgcaaacaga ggagctgtgg cgaaggcgat gaatgccagt
     3061 gcggagaaca attttttcat gttagatatc attttgagtt tttaatagcg cattgtctgt
     3121 ccacaaatat agctattcgc agaaagacaa caccgaaatt caacaaaaac cataccaagc
     3181 accacggttc tgcaggttat tttttccggc cgaaggaccc gtttcttatc tttgtagcat
     3241 gtcatccaac gtacaatcag acaacctttc ctatacagaa ttcaaacagc aggtcctggc
     3301 tgactaccgc ctggcctgcc tgagccgtga agccagtctg ctgggccgca aggaagtgct
     3361 gacaggtaag gccaagttcg gcatcttcgg cgacggtaaa gagatcgccc agctggccat
     3421 ggcccgtgtc tttgaagacg gcgatttccg gtcgggctac taccgcgacc aaaccttcat
     3481 gttcgccgcg ggtatggcca ccatcgaaca attctttgcc cagctgtatg ccatccctga
     3541 tgtggaggag gatccgttct cagccgggcg acaaatgaac gctcatttcg ccacccgcag
     3601 cctggatgca caaggaaact ggaaggaact gcgctccatg aagaacagca gttctgacac
     3661 atctcccacc gccagtcaga tggtcagagc ccttggtctc gcactggcat cgaagaaata
     3721 ccgcgacgta ccagcactga acgatacgcc tttctctttc tccggtaatg aagtggtgtt
     3781 cgccaccatc ggcgatgcat cgacctctga aggggttttc tgggagaccg tcaatgcggc
     3841 tggtgtactc aaagtgccac tggcattctt cgtctgggac gatggctacg gcatctccgt
     3901 tccccgcgaa taccagacca ccaaagacag catttccgaa gccctgtcgg gcatgcagct
     3961 cgatgaagac ggtaacggca tacgcatcta tgaagtgaag gcgtgggatt atccctccct
     4021 catccagacc ttcgaagacg gtatcagttt ctgtcgcgag aaccatattc cggtcttctt
     4081 ccacgtgaag gaagtgaccc agccccaggg gcacagtacc tctggctcac atgagcgcta
     4141 caagagcccg gagcgactgg attgggaagc agaatacgac ggcatcaaaa aaatgcggga
     4201 gtggatgtct gcccacggcc tggcctctga agaagagatg gaccagatag ccgaagaagc
     4261 caaaaaggaa gttcgcgaga tccagaagtc ggcctggaac aagcatctga tgtctataaa
     4321 agccgacaag gatgtcgtca ttaagctact atctgacctc gcctccaccg gccatgagca
     4381 ggtagctgct gtgcagaagc aactggaaac ggctatggat cccatccgcc gcgatgtgat
     4441 gagagctgcc agacaggcat tgtatgccac cgccggacag gactccgccc agcgccaggc
     4501 actgaagcag tgggtggacg actatgctgc tgtcaaccac gaccggtaca atagttattt
     4561 gttcagccag agcagtcagg cggtcaccaa tatttctgtg gtacccgcag tctatggcga
     4621 cgatgcaccg ctgaagaatg gttttgagat cctgaacacc tgctttgaag agaacttcaa
     4681 acgctacccc gggttgttcg catttggcga agacctcgga aagatcggag atgtgaatca
     4741 gggttttgca ggcctgcagg agaaattcgg tgaagaccgg atcttcgatg tgggcatccg
     4801 cgaggcgacc atcatcggtc aggggatagg catggccatg cggggattgc ggcccattgc
     4861 tgagatacaa tacctcgatt atctgatcta cggattgcag cccatcagtg atgacctggc
     4921 tacactgcaa tatcgcacca aaggcggaca aaaagcaccg gtcatcatcc gtacccgcgg
     4981 tcaccgcctg gaaggtatct ggcataccgg ttcaccgatg gcgatgatac tcggtacctg
     5041 tcgcggcgtg cacctctgcg taccacgaaa catggtacag gctgcaggta tgtacaactt
     5101 gttgctccag agtgacgaac ctgctattgt ggtggagtgt ctgaacggct atcgcctgaa
     5161 ggaaaagatg ccggacaata tcgccgactt caccgtgccg ctcggtgtac ccgagatcat
     5221 gcgctatggt gatgacatca ccgtggtcag ctatggttct actttgagag tggtggaaga
     5281 agcctgcaac gacctggaaa aactgggcat gtccatcgag ctcatcgatg cacagaccct
     5341 gctgccattc gatatctatg gcgtgatcgc acagagtatc cgcaagacga accgggtcat
     5401 cttcattgac gaagatgttc cgggtggggc cactgcctat atgctggatc aggtgttcaa
     5461 ggacaaagaa cttttccagt acctggatgc cctgccacag acactcagtg cacaacccaa
     5521 ccgctcagct tatgccagcg acggcgatta cttctgcaaa cccaacgcag aagacatcta
     5581 cgaagccatc tataagatga tgagtgatgt ggatccggat agttatccgg agatataggt
     5641 atcaggtagc tagtatcagg tatcaggtat caggtatcgt gtagcaagta ttgattcact
     5701 cccgatagct atcgggaagc tcctgcgtcg cccatatcag gaatctcctg tctcagtttc
     5761 cgttcattca agtaaattgt tggtcaattg atcagtaaga gattcccgct ttcg
//
LOCUS       DAOMAX010000168         5769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2272_length_5769_cov_4.284564, whole genome shotgun sequence.
ACCESSION   DAOMAX010000168 DAOMAX010000000
VERSION     DAOMAX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2272_length_5769_cov_4.284564"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..467)
                     /locus_tag="PK742_08975"
     CDS             complement(15..467)
                     /locus_tag="PK742_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76760.1"
                     /translation="MSDYNEQGGQYVWVSNGGCQICQTMEGTYATEPARPHYYCDCEI
                     SFVRNSDRENTDNNCRLAEEEISWEGAGAGEHAWDNNDPTFPVVYKFTVTCRNGSSFS
                     ADIEIDLDRQEVMDDFEMAYEAGLSEALAELDAEISSRCQPCNEQLLV"
     gene            complement(464..1522)
                     /locus_tag="PK742_08980"
     CDS             complement(464..1522)
                     /locus_tag="PK742_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU76761.1"
                     /translation="MSDQYRPIEDLDGIGRVYGQKLRALKVDLIRDMIWYAPSVLHRL
                     SAIPLKDLYRYRSTALLLEVRNMTLTSAEVLAAADIFSSAELQTKNTTEVMELLKKGK
                     VRIKENLVADMIADARLLHYTGTLTGRVVDKRGRSMKEVTVSCGPYSTKTDTYGRFRF
                     YKLPAANTYPVQLAMEGKEPMVVDHLQVYAQKQIPDLQTITYTPVKLKVSKGDEYDGD
                     TMPSLFKPSYKIITRTDALRVFDYLSVFEVKEGMVTLVSRYRAWDDQTVFIHQWKVEA
                     PHFPADTPKGTIVRILASGELVPARFSSSHRKLNSALQQTREAFANRAKPTTPMELEA
                     YMREYSETFGQHFNNQRS"
     gene            complement(1676..3028)
                     /locus_tag="PK742_08985"
     CDS             complement(1676..3028)
                     /locus_tag="PK742_08985"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQU76762.1"
                     /translation="MLKRILFTLLITAFFTPAFSQYRIPPEWHTHSATWLQWPHDSTY
                     YIGYRDFFEPAWIDMTLALDGSEQVFIIAYDSLELEHITSILADAGADMDSLQFFVFP
                     NNDYWVRDNGPIFVLDSLGQTAITDWNFDGWGDDAPYTLCDVIPASIGGAIGIPVIDL
                     SAYTLEGGAVEHDGNGTFIGTISSILGDGRNPGLTTDDMEEVMQEYLGFEQFIWLEGA
                     YGGGFDITDSHIDGFLRFRDEHTIVTMNTADLNYWFVNAADRDLIFNALNTDGEAYNY
                     VYLPLTDKNVKTTEGMNTGSKGSYVNYYEGNQVVLVPVYADDNDSLALSIMQDLYPDK
                     EVIGIDCRNMFYWGGMVHCVTQQQPASEVITTSVSAIHKDLQLLVYPNPASEWLFVQT
                     PFQSGKLCIQDMQGRVLWRETLSQGQYELPVSHLAEGRYLLTVSYGERMIRSSFVVQH
                     "
     gene            complement(3022..3516)
                     /locus_tag="PK742_08990"
     CDS             complement(3022..3516)
                     /locus_tag="PK742_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76763.1"
                     /translation="MHLIAAYLTARLLCTPADTIIHIPDPGEVGMYCLQYDTFRLEVQ
                     TETSMTVMAEGPHCDLRNWKHGYSQLFDITYMVQPNGSLEMPDLPKAALDVFPAVSQK
                     EMQDAVRQYCDDFWPPGIEKSKTPHTYPFGVSPSRYIFKLSGTLKNGQQQTIIFLMQV
                     DMGC"
     gene            complement(3546..>5769)
                     /locus_tag="PK742_08995"
     CDS             complement(3546..>5769)
                     /locus_tag="PK742_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76764.1"
                     /translation="AHVYRTRLHNGKRTIPRRYLLDACRIHPSLYRLAMEYLPLYFSR
                     RHGDPSRPWNEFNIHTQTETGEQLFDYEGNWRDIFQNWEALARSFPEYTSAMIARFLN
                     ATTADGYNPYRINRDGMDWEIADPNDPWSHIGYWSDHQIIYLLKLLETSEQFYPGKLQ
                     LGLDDRIYAFANVPYRILPFDNMLQDPHDTIHYDQQEADRIDNRFELIGSDGKLLWDS
                     DGRIIHTTLAEKLLLPLLAKLSNYVHEGGIWLNTQRPEWNDANNALVGNGLSVVTLQY
                     MRRYVQFLLGIYPATGTLTVGAEMKGWLEELHAIFQQHETDLKKPFTDAKRMQMMSRL
                     GHAAQHYNRQVYNGLSGYTANLHNKKLNEFLQLVLSYIDVTLHANKRSDHLYHAYNLM
                     KVDTNAGTVTIEHMYLMLEGQVAALSSGRLLPEEAHLLLDALKDSPLYREDQYSYLLY
                     PDRQLPTFIHKNTFSGQGHETLLHQLDALPGQPIVSKDAAGNWHFHKDIANADHLEQK
                     LEDCLKISGWQPDEAQRSDLLNLYESVFQHHRFTGRSGTFYGYEGLNCIYWHMVSKLL
                     LAVQENILKAYDIAADAVTTGKLIEHYYEIRAGIGLNKTPAVYGAFPTDPYSHTPGNK
                     GAQQPGMTGQVKEDIICRFGELGLVVQDGCIRFHPGLLRKNEFIATADDATLLFTFCG
                     TRICYHLTEQSPRIMITDVHQHIHEITGSTLDQEWSLEIFGRSGKISCIDVFIAPAIA
                     "
BASE COUNT         1352 a         1415 c         1487 g         1515 t
ORIGIN      
        1 agcagatctg cacatcaaac gagcagttgt tcattacatg gctggcaacg gctgctgatc
       61 tctgcatcca gttcagccag tgcttcggat aagccggctt cataggccat ttcaaaatca
      121 tccatcactt cctgtctgtc caggtctatt tcgatgtcgg cgctgaagct gctgccgttg
      181 cgacaggtca ccgtgaattt gtacaccacc ggaaaagtgg gatcgttgtt gtcccaggca
      241 tgctcgccgg caccggctcc ttcccaggag atctcctctt cggccagacg gcagttgttg
      301 tcagtattct cccggtcgct gttgcgcacg aagctgatct cacagtcgca gtagtaatgc
      361 ggtcgggcag gttctgtggc ataggtacct tccatggtct ggcagatctg gcatccgccg
      421 ttggacaccc atacatattg tccgccttgt tcgttgtagt cgctcatgat cgctggttgt
      481 tgaagtgttg accaaaggtt tcgctgtatt cccgcatgta ggcttccagt tccatcgggg
      541 tagtgggttt cgcccggttg gcaaatgcct cgcgggtctg ttgcagggcg ctgttgagtt
      601 tgcggtgact gctgctgaaa cgggccggca ccagttcgcc gctggccagg atacgcacga
      661 tggtaccctt aggggtatcg gccgggaagt gaggtgcttc tactttccac tggtggatga
      721 aaacggtctg gtcatcccag gcccggtacc tgctgaccag ggtgaccatc ccctccttca
      781 cttcaaagac actgagataa tcaaacaccc gcaacgcatc tgttcttgtg atgatcttgt
      841 aggatggttt gaacagagat ggcatggtgt cgccatcata ttcatcgccc ttactgacct
      901 tcagtttcac aggtgtgtag gtgatggtct gcagatcagg tatctgcttc tgggcataga
      961 cctgtaagtg atccaccacc atcggttcct tgccttccat agccagttgt acaggatagg
     1021 tattcgctgc cggcagtttg tagaaccgga aacggccgta tgtatctgtt ttagtagagt
     1081 acggtccgca ggaaacagtt acttccttca tgctgcgccc tcgcttgtcc accaccctac
     1141 ctgtaagggt acctgtgtag tgcagtagcc tggcatcggc gatcatatca gctacgaggt
     1201 tctcttttat gcgcactttg cccttcttca gcagttccat cacttccgtg gtgttcttgg
     1261 tctgcagttc agcggaagag aaaatgtcag cggcagccag aacctctgca gaagtcagtg
     1321 tcatgttgcg cacttccagc aacagggctg tacttctgta ccggtagagg tctttgaggg
     1381 ggatggcgga gagccggtgc agcacagaag gtgcatacca gatcatatct ctgatcaggt
     1441 ccactttcag tgccctcagt ttttgtccgt acacccggcc gataccgtcc aggtcttcga
     1501 tgggtctgta ttgatcgctc atagtatata tagttgccgc ctgtcagttg aactgaccgt
     1561 ttcctgcgga agcaaaaagg tagaaagaaa gcatcaattg ctgctatcct tacatgaaaa
     1621 tcttcaagta cctgcctata agacaaaggc aactgtgatc agtcagcaaa tcatttcagt
     1681 gctgtaccac aaaggaagac ctgatcatgc gttctccgta actgacggta agcaggtatc
     1741 tgccttcagc cagatgactt acaggtaatt catactgacc ctgagaaagt gtttcccgcc
     1801 acaggacccg gccctgcata tcctgaatac agagttttcc tgactgaaaa ggggtttgca
     1861 cgaacagcca ttcagaagcc ggattggggt acaccagcag ctgcagatcc ttgtgaatgg
     1921 cagacacact ggtggtgatc acttcagagg ccggttgctg ttgggtaacg caatgcacca
     1981 taccacccca atagaacata tttctgcaat cgatcccgat cacttcctta tcggggtaga
     2041 ggtcttgcat gatggataat gccaggctgt cgttgtcatc ggcatacaca ggaaccagaa
     2101 caacctgatt cccttcatag tagttgacat aactgccttt tgacccggta ttcatacctt
     2161 ctgtggtctt gacgttcttg tcggtaagcg gaagatagac atagttatac gcttcgccgt
     2221 ctgtattcag ggcattgaat atgagatcac gatcggcagc attgacaaac cagtaattaa
     2281 ggtcagcagt gttcatggtg acgatggtgt gctcatcgcg aaagcgaaga aatccatcta
     2341 tatgagaatc agtgatatca aaacctccgc catatgcacc ttccagccag atgaattgtt
     2401 caaaaccaag atattcctgc atcacctctt ccatatcatc tgtggtgaga ccggggtttc
     2461 taccatcacc cagtatagaa gatatggttc cgataaaggt gccgttgccg tcgtgttcaa
     2521 ccgcccctcc ttccagggta taggccgaga ggtcaatgac cggtatgccg atggctcccc
     2581 caattgatgc aggaatgaca tcacacaaag tataaggggc atcgtctccc cagccatcaa
     2641 aattccagtc ggtaatggcg gtttgaccaa gcgaatccag gacgaaaatg gggccgttat
     2701 cacgcaccca gtagtcgtta ttcggaaaaa cgaagaactg caggctgtcc atatctgcac
     2761 cggcatctgc cagtatggag gtgatgtgtt ccagttccag gctgtcatag gctatgataa
     2821 agacctgttc gctgccatcc agtgccaggg tcatatctat ccaggcgggt tcaaagaaat
     2881 ctctgtaacc gatatagtag gtggagtcat gaggccactg caaccaggta gcgctgtgtg
     2941 tatgccactc agggggtatt ctgtactgcg agaaagcagg agtaaaaaag gccgtgatca
     3001 acagcgtaaa taagattctt ttcagcatcc catatccact tgcattagaa aaatgatggt
     3061 ttgttgttgt ccgttcttca gggtaccgga cagtttaaag atatacctgc ttgggcttac
     3121 cccaaagggg taggtatgag gtgtcttgct tttttcaatt cctggcggcc agaaatcatc
     3181 gcagtattgt ctgacagcgt cctgcatctc tttttgtgag acagcaggaa acacatccaa
     3241 cgcagctttt ggcaggtcag gcatttccag gctaccgttg ggttgtacca tatatgtgat
     3301 atcaaacaac tgagagtacc cgtgtttcca gttgcgcaga tcacagtgcg gaccttcggc
     3361 catgaccgtc atgctggttt cagtctgcac ttccagcctg aatgtgtcat attggaggca
     3421 gtacataccc acttcgcccg gatcgggaat gtggatgatg gtatctgcag gtgtgcacaa
     3481 cagacgggcc gtaagataag ctgcaatcag atgcatgcaa catcaaggta acaagaaaaa
     3541 taaattcagg caatggcagg tgcaatgaac acatctatgc aactgatctt acctgatctg
     3601 ccaaagatct ccagactcca ctcctgatca agtgttgagc cggttatctc gtgtatatgc
     3661 tgatgcacat ctgtgatcat gattcggggt gattgttctg tcagatgata acagatgcgg
     3721 gttccgcaga aagtgaaaag cagtgtggcg tcatctgctg ttgcaatgaa ttcatttttt
     3781 ctcagcaggc cggggtggaa ccggatacaa ccgtcttgta ccaccaggcc gagttctccg
     3841 aagcgacaga tgatatcttc cttcacctgt ccggtcatac cgggctgctg ggcacctttg
     3901 ttgccgggag tgtgtgaata gggatcggtg gggaaggcac catatacagc aggggtctta
     3961 ttgagcccga tgcctgcacg aatctcatag tagtgttcaa tcagcttgcc tgtagtaacg
     4021 gcatctgcag cgatatcata ggctttcaga atgttttcct gcacggccag tagcagcttg
     4081 gacaccatat gccagtagat gcaattcaat ccttcatatc catagaaggt gcctgacctg
     4141 ccggtgaaac gatggtgttg aaatacagat tcatacaggt tcagcaggtc agatctctgg
     4201 gcttcatccg gttgccatcc tgagatcttc aggcagtctt ccagtttttg ttccaggtgg
     4261 tcagcattgg ctatgtcttt atggaagtgc cagttgcctg cagcgtcttt tgatactatg
     4321 ggttgcccgg gcagagcatc aagctgatgc agcaaagttt catgaccttg tccggaaaag
     4381 gtattcttat gtataaatgt tggcaactgc ctgtcagggt acagtaaata gctgtactgg
     4441 tcttcccggt acagcgggct gtctttgagt gcatccagca aaaggtgtgc ttcttcgggc
     4501 aacaatctgc ccgaactgag tgctgccacc tgaccttcca gcatcagata catgtgttca
     4561 atggtaacgg tgcctgcatt tgtatcaacc ttcatcaggt tgtatgcatg atacaggtga
     4621 tcactgcgtt tgttggcgtg cagtgtaaca tctatatagg acagtacgag ctggagaaac
     4681 tcattcagct ttttgttatg cagatttgcg gtatagcctg acagtccgtt atatacctgg
     4741 cgattgtaat gctgtgccgc atgacccaga cgagacatca tctgcatgcg tttcgcatca
     4801 gtgaaaggct ttttgagatc tgtttcatgc tgctggaaga tggcatgtaa ttcttccagc
     4861 catcccttca tttctgctcc aacggtcaat gtacctgttg ccggatagat gccaagtaaa
     4921 aactgtacat agcggcgcat atactgcagc gtgaccacag acagtccgtt ccccaccagg
     4981 gcgttgttgg catcattcca ttccggacgc tgggtgttca gccagatgcc accttcgtgt
     5041 acatagttgc tgagtttcgc caggagcgga agcagcagtt tttctgccag ggtggtatga
     5101 atgatacgcc cgtcactgtc ccacagcagc ttgccatctg aaccgatcag ttcaaaacga
     5161 ttgtcaatac ggtcggcttc ctgctgatca tagtgaatgg tatcatgcgg atcctgcagc
     5221 atgttgtcaa atggcaggat gcgatacggc acattggcaa aagcatagat cctgtcatca
     5281 aggccgagct gcagttttcc cgggtagaac tgttcagaag tttccagcag cttgagcaga
     5341 tagatgatct gatgatcact ccaatagccg atgtgcgacc agggatcgtt cgggtctgcg
     5401 atctcccagt ccataccgtc gcggttgatc cggtaagggt tatagccatc tgccgtggtc
     5461 gcattgagga aacgggcaat catggccgat gtgtactccg gaaaagaccg ggcaagggct
     5521 tcccagttct gaaagatgtc acgccagttt ccttcgtagt caaaaagctg ctctccggtt
     5581 tctgtctggg tatggatgtt gaattcattc cacgggcggc tcggatctcc atgtcttctg
     5641 ctgaagtaca gaggcaggta ttccatggcc agccggtaaa gagaaggatg gatacggcag
     5701 gcatccagca gatatcggcg ggggatggtt cgttttccgt tgtgcaaccg ggtacggtac
     5761 acatgagct
//
LOCUS       DAOMAX010000169         5719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2301_length_5719_cov_4.565678, whole genome shotgun sequence.
ACCESSION   DAOMAX010000169 DAOMAX010000000
VERSION     DAOMAX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2301_length_5719_cov_4.565678"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..451)
                     /locus_tag="PK742_09000"
     CDS             complement(215..451)
                     /locus_tag="PK742_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76765.1"
                     /translation="MADKPYLPIACNLYDRLTDLATHGHKVSLVVRTGTDEVSWNDVI
                     ITDIVTRNKEEFLVTACGRECRLDHIIQLADTGQ"
     gene            683..1150
                     /locus_tag="PK742_09005"
     CDS             683..1150
                     /locus_tag="PK742_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HQU76766.1"
                     /translation="MLPNSQIDTLDKEILEILMRDATIPYTDIAKQLKVSSGTVHVRM
                     KKLTQLGIIRSVNLHVNPEKLGFDITAFLGVYLEKSSMYDAVVKELQGIPEVVECYYL
                     TGNYSIFVKIYCRDATHLKDVLHDKVQRIAGIDRTETFISLEQTFTRPMHIDK"
     gene            complement(1153..3954)
                     /gene="polA"
                     /locus_tag="PK742_09010"
     CDS             complement(1153..3954)
                     /gene="polA"
                     /locus_tag="PK742_09010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQU76767.1"
                     /translation="MADNNDKKLYLLDAFALIFRAYFAFSRNPLINSKGQNVSAVTGF
                     VNTLYDLLKNTDSTHIAVCFDSDQAPVGREEVYADYKANREAAPEDIKWSVPIIKQIV
                     EAFHIPILEVPGFEADDVVGTLAKKAEQAGYQVYMVTMDKDYGQLMSENIFMYRPSYT
                     GKGFDTVSVTEMMEKWGIEDPLQLIDILGLMGDSVDNIPGVPGIGEKTAQKLIAEYGS
                     VENVLANTDKMKGKLQENLRTYADQALMSKQLATIILDVPVEFNEEELVIEEPDKEKL
                     AEIFAELEFRQLGKRILGDSYQVNVARTGEQIDIFGGSSGGTEIKQASEQVAGGKDIT
                     NTEHRYTLCNDDKSIDQLIKDLQKQKLICFDTETTGVDANEAELVGMSFSWEAGSGYY
                     VPVPADRSSADALLNKFRPVLESEKIGKVAQNLKYDYLIMKWYGVELKGDLHDSMLAH
                     YLLEPELRHNMDYLSENYLGYTPVSIETLIGKKGKNQLSMRDAPLDKVAEYAAEDADI
                     TLQLHTIFQPMLDKEELRKLYEDVEMPLVPVLAAMEYEGVNLDVDFLNKYSEELAVDI
                     EIAAKEIYELAEVKFNIDSPKQLGEVLFTKMKIPYEGKKTKTGQLSTSEDILARLAPN
                     HPIANHILEYRELNKLKSTYVDALPQLINPKTGRVHTNFGQAIAASGRLSSNNPNLQN
                     IPIRTERGQRVRKAFIPRDEHHIILSADYSQIELRIIAALSNDEGMIEAFRQGMDIHS
                     ATAARVFGVALAEVNRDMRSKAKAVNFGIAYGQTAFGLSQNLGISRTEAKEIIDNYNE
                     KFPGVARLMEENIEYARKHGYVKTVLGRKRYLRDINSANNTVRSAAERVAVNSPIQGS
                     AADLIKVAMLHLHDAMNKRHMRSRMTLQVHDELVFDAHRDEVEELEVLVREKMVNALS
                     LNVPIEAEVGKGSNWLEAH"
     gene            complement(3957..4304)
                     /locus_tag="PK742_09015"
     CDS             complement(3957..4304)
                     /locus_tag="PK742_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQU76768.1"
                     /translation="MSQLIEEFNDYRARMNEVILGKNNKVINRLFNLDTNTYADGALP
                     VSTKEMLGLVASMVLRCDDCIKYHLGKCHEEGVTTEQVYEVFAIANIVGGTIVIPHTR
                     RAAEYWEELMENN"
     gene            4374..5045
                     /locus_tag="PK742_09020"
     CDS             4374..5045
                     /locus_tag="PK742_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQU76769.1"
                     /translation="MKSEFAIFEKTPKTMTDTTPASPRRNAFPRWLVPAAVILVLGII
                     LYNFFVGAQRRMVEKDEVANEKWAAVESSYQRRADLIGNLVATVKGYADFEQETLTKV
                     IEARSKATSITIDPSNTTPEQFAQFQQAQDGLSSALSRLLVTVERYPDLKANQNFLEL
                     QAELAGTENRINVARNDYNTAVKEYNTYIRRFPQSFLAGMYGFEKRTMFKAAEGSENA
                     PKVEF"
     gene            5049..5486
                     /locus_tag="PK742_09025"
     CDS             5049..5486
                     /locus_tag="PK742_09025"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQU76770.1"
                     /translation="MPDPATFFTDTEKQELVRTIRTAEKLTSGEIRLHVEERCKGDAF
                     QRGLQVFAQLKMHRTAARNGVLFYLATADRKFSIVADEGINNMVSEGFWNEIKDTMQA
                     KFQQGEFMLGLALGIERAGQQLQLHFPYQRDDENELPDEISFG"
     gene            5483..>5719
                     /locus_tag="PK742_09030"
     CDS             5483..>5719
                     /locus_tag="PK742_09030"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQU76771.1"
                     /translation="MRRSLVLVLIMFLLHPVFAQVNEKDLPPRPTPPRLVNDLADVLS
                     PSQEQMLEQKLVAFDDSTSTQIAVITLRSTGQYVI"
BASE COUNT         1375 a         1450 c         1380 g         1514 t
ORIGIN      
        1 ggaatgccat atcgttcgcc aattaggaag tacacaaagc agaacaggat gaaccagcca
       61 agtaccagaa agtggttgcg cttcagatgc aacagcagca gctgcacgga gaacgaaaac
      121 ctgatacgcc tgaacatttc tgccatactc cagtaggtgt acgggaaata tagccaaatg
      181 gtcaggtaat cagaaaatta tggcgtaagg ctgactattg cccggtatca gccagctgaa
      241 tgatatggtc cagacggcac tctctgccgc aggctgttac caaaaactct tctttattcc
      301 gggtcacgat atcagttatg atgacatcat tccaggagac ctcatcagta ccggttctga
      361 caaccaggct gactttgtgc ccgtgtgttg ccagatctgt aagccggtca tacaagttgc
      421 aggcaattgg taagtagggt ttatcagcca ttttgatgca gataaaacaa ggtaagcaac
      481 agaatggtgc cgtcagggca aaaaaaaatg ctgcctgctc ctcatcagac agcattataa
      541 aaaaggggag atttaatgcg ttacagcaaa aataacacag gaattgaatt gtccaaatat
      601 ttttacaaaa atttgaaaaa ttataagaaa cctctaataa atttgcccga tccttgcata
      661 ttgctaaacc tgaaacacca ctatgctgcc caattcacaa attgataccc ttgataagga
      721 gatcctggag atcctgatgc gtgatgccac gatcccctat acggatatcg ctaaacagtt
      781 gaaagtgtcc agcggaaccg ttcatgtgcg gatgaagaaa ttgacccagc tgggcatcat
      841 ccgctcggtc aatctgcatg tcaatccgga aaaactcgga tttgacatca ctgcttttct
      901 gggagtatat ctggagaaga gttctatgta tgatgcagtg gtaaaagaac tgcagggcat
      961 tccggaagta gtggaatgtt attacctgac cggtaactac agcatcttcg tcaagatcta
     1021 ttgccgtgac gccacccatc tgaaagatgt cttacacgat aaggtacagc gcatagcggg
     1081 cattgaccgc acggaaacct ttatctctct ggagcagacc ttcacccgac ccatgcatat
     1141 agataagtaa ggtcagtggg cttccagcca gttgctgcct tttcctacct ctgcttcaat
     1201 gggcacgttg agtgacaatg cgttcaccat tttctctctg accagtactt ccagttcttc
     1261 cacctcatcg cggtgtgcgt cgaacaccag ttcatcatgc acctgcaggg tcattctgct
     1321 gcgcatgtgc cttttgttca tcgcatcgtg caggtgcagc atagcaactt ttatgaggtc
     1381 tgccgcactt ccctgtatag gagagttgac cgctactctt tcggcggctg aacgcacagt
     1441 gttgttggca gagttgatat cacgcagata gcgtttcctt cccagcacgg tcttcacgta
     1501 tccgtgcttg cgggcatact cgatgttctc ttccatcagc ctggccacac ccggaaactt
     1561 ttcattgtag ttgtctatga tttctttggc ttcggtgcga ctgataccca ggttctgaga
     1621 cagaccgaat gcggtttgcc cataggcgat accaaaattc acggcctttg ctttggaacg
     1681 catgtcgcgg ttcacttctg ccagtgccac tccaaacaca cgtgctgctg tagctgagtg
     1741 aatgtccatt ccttgtctga aagcctcgat catgccttca tcgttactca gcgctgcaat
     1801 gatgcgcaat tcgatctgcg aatagtcggc gctgagaatg atgtggtgct catctctggg
     1861 gatgaatgct tttcttaccc tttgcccgcg ttcggtgcga atggggatgt tctgcaggtt
     1921 gggattgttg gaactcagcc tgccactggc ggcgatggcc tgtccgaaat tggtatgtac
     1981 cctgcctgtt ttcgggttga tcagctgtgg caatgcatcc acataggtgg acttcagctt
     2041 gttgagctcg cggtactcca gaatatggtt ggcgatggga tgattgggcg caagcctggc
     2101 caggatgtct tcactcgtgc tgagctgtcc ggtcttggtc ttcttgccct cataagggat
     2161 cttcattttg gtgaagagta cttctcccag ctgctttggt gagtcaatgt tgaacttcac
     2221 ctccgccagt tcatagatct ctttggcagc tatctcaata tcgaccgcca gctcttccga
     2281 atacttgttc aggaaatcca catccagatt cactccttca tattccatgg cggccagcac
     2341 cggcaccagc ggcatctcta catcctcata taatttcctg agttcttcct tgtccagcat
     2401 aggctggaag atggtatgca gctgcagggt gatgtcggca tcttcggctg catattccgc
     2461 aaccttgtcc agcggggcat cacgcatgct cagctggttc ttgccttttt ttccaatgag
     2521 tgtttcgatg gagaccggcg tatatcccag gtaattttca gacaggtaat ccatgttgtg
     2581 tcgcaactcc ggttccagca gatagtgggc cagcatgctg tcgtgcagat cacctttcag
     2641 ttctacgcca taccacttca tgatgaggta atcgtacttc aggttctggg ctaccttgcc
     2701 gatcttttct gattccagca cagggcgaaa tttgttcagc aatgcatctg ccgatgatct
     2761 gtcggccgga acgggaacat aatatccgct gcctgcttcc caactgaagc tcatccccac
     2821 cagctctgct tcgttggcat ctacaccggt ggtctctgta tcaaaacaga tcagcttttg
     2881 cttttgcaga tctttgatca actggtcgat ggacttgtcg tcattacaaa gggtgtaccg
     2941 gtgctctgtg ttggtgatat cctttccccc cgcaacctgt tcactggctt gtttgatctc
     3001 tgtgcctcca gaactgcccc cgaaaatatc tatctgttcg ccggtgcggg caacgttcac
     3061 ctggtaactg tcgcccagga tacgtttacc cagctgcctg aattccagtt ctgcgaagat
     3121 ctctgccagc ttttccttgt cgggttcttc tatgaccagc tcctcttcat tgaattctac
     3181 cggcacatcc aggataatgg tggccagttg cttagacatc aaagcctggt ctgcgtaggt
     3241 gcgcaggttc tcctgcagct tgcctttcat cttgtcggta ttggcaagta cattttctac
     3301 ggatccgtat tcagcaatga gcttctgagc ggtcttctca ccaataccgg gcacaccggg
     3361 aatgttgtcc acactgtcgc ccatcagacc caggatatca atgagttgta atggatcttc
     3421 tatgccccat ttttccatca tctccgttac actgaccgta tcaaatcctt ttccggtgta
     3481 ggacgggcga tacatgaaga tgttctcact catgagctgc ccgtagtctt tgtccatggt
     3541 gaccatatat acctggtagc cggcctgttc tgcttttttt gcgagggttc ccaccacatc
     3601 atctgcttca aatccgggta cttccagaat aggaatatga aacgcttcca cgatctgttt
     3661 gatgatgggt acactccatt tgatgtcttc cggagcagct tcgcggttgg ctttgtaatc
     3721 tgcatagacc tcttctctgc ccaccggcgc ctggtcactg tcaaagcaca ctgcaatatg
     3781 cgtactgtct gtattcttca gcagatcata cagcgtattg acaaacccgg taactgcaga
     3841 gacattctgt cctttactgt tgatgagcgg gttacgggag aatgcgaagt aggcccggaa
     3901 gatcagggca aatgcgtcga ggaggtataa tttcttgtcg ttgttatctg ccatgatcag
     3961 ttgttttcca tgagttcttc ccagtattct gcagcccgtc tcgtgtgcgg tatgacaatg
     4021 gtaccaccca cgatattggc gatggcgaat acttcataga cctgttcggt ggttactcct
     4081 tcctcatggc acttgccgag gtggtatttg atgcaatcat cgcagcgaag caccatactg
     4141 gccaccaggc ccagcatttc tttggtgctg acaggcaggg caccgtctgc ataggtattg
     4201 gtgtccaggt tgaacaggcg gttgatgacc ttattgttct tgcccagaat gacctcattc
     4261 atgcgggcac ggtaatcatt gaattcttcg atgagctggc tcatagtgtt ggtttgggtg
     4321 ggagggtaaa aataaaagaa taagcccttt cattcgtctt acccgataca ttcgtgaaaa
     4381 gtgaattcgc tatctttgaa aaaacaccaa aaaccatgac cgatacaact cctgcttccc
     4441 cccgcagaaa cgcatttcca cgctggctgg tgccggccgc cgtcattctg gtactgggta
     4501 tcatactgta taacttcttc gtgggtgcac agcgccgcat ggtagagaag gatgaggtgg
     4561 ccaatgaaaa atgggctgca gtagaaagct cttaccagcg ccgtgccgac ctgatcggaa
     4621 accttgtagc tactgttaaa ggctatgccg attttgaaca ggagaccctg accaaagtga
     4681 tagaagcccg ttccaaagct accagcatca ccatcgatcc cagtaatacc accccggaac
     4741 aattcgccca gttccagcag gcacaagacg gactgagttc tgcactgagc aggttattgg
     4801 tcactgttga aagataccct gacctgaaag caaaccagaa tttcctggaa ttgcaggctg
     4861 aactggcagg cacggagaac aggatcaatg tagcccgcaa cgattataat acagcggtca
     4921 aggagtacaa tacctacata agaagattcc cgcagagctt cctggctggc atgtatggct
     4981 ttgaaaagcg taccatgttc aaggctgcag aaggctcaga aaatgcgccc aaggtagaat
     5041 tctgatccat gcccgacccg gcaactttct ttaccgacac ggagaaacag gaactggtaa
     5101 gaaccatccg gacggctgaa aagctgacct ccggagagat ccgtctgcac gtggaggaac
     5161 gctgtaaagg cgatgccttc caacggggtt tacaggtatt tgcccagctg aagatgcaca
     5221 gaacagcagc acgaaacggg gtgctgttct acctggctac ggccgacagg aaattctcca
     5281 ttgttgccga tgaaggcatc aacaacatgg tatccgaagg cttttggaat gagatcaaag
     5341 acaccatgca ggcgaaattt cagcagggcg aattcatgct ggggctggcg ctgggtatag
     5401 aacgggcagg tcagcaattg caactgcatt ttccttatca gcgggatgat gagaacgaat
     5461 taccggacga aatctctttc ggatgagacg cagtttggtt ctggtgttga taatgttctt
     5521 attgcatcct gtctttgcac aggtcaatga aaaggatctt ccgcccagac ccactcctcc
     5581 acgactggtc aacgacctgg ccgatgtact gagcccgtcg caggagcaga tgctggaaca
     5641 aaaactggtg gcctttgatg attccacctc cacacagatc gcggtcatca ctctgcgaag
     5701 taccggacaa tacgtaata
//
LOCUS       DAOMAX010000170         5695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2313_length_5695_cov_4.339362, whole genome shotgun sequence.
ACCESSION   DAOMAX010000170 DAOMAX010000000
VERSION     DAOMAX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_5695_cov_4.339362"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PK742_09035"
     CDS             complement(<1..1755)
                     /locus_tag="PK742_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76772.1"
                     /translation="MRTTLLAAFMISSCLCYAQEPPVLIQAEKITGSILQHAVTEKEL
                     LIDRNRNYFTELTASAHDSVYFSEPELRRSSSLLQSLEGIYSDYLRSDNNIAVGPEHV
                     VQVINSSYLSSLIRVWDKAGNILIDKSLFKDLTGDFDWGDPNIIYDWEADRFVITFLF
                     SEEDQKLIIAVSDSNDPTGSWTGYSFITPGKWPDYEKLGRWGNSYVITTATSHPAVYL
                     VNRDQLLSGEETLDVVSLSVLRIPGMSWQGLSPIHQTGYNPLPDARPPSVARIVEGSY
                     YPEDSVADRLEIFSFQINWADPLAATVTGPVIIPVMSFDHSVCDSVTPNACLEQPGTD
                     VRIWPCEDFLMDKPQMVIMDGNLHVVCTHVVNAGDSVAGMKWYELLCDASGSWSLVQE
                     GLQSPDENHRFMGSISINEDGVIALGYNIVSSTVFPGFRAATHRPCDPQGTMGEEITG
                     ATGSAPNASNSYGDYNSMVSDPTDGSFWFTAQYNASSKWSTRVIQFSADSCTTDQIET
                     SANTIVQWYPNPANNLLHVSCKEPGTWYIRITDQSGKDIYISSFYDQTNVVLDSFPAG
                     LYIGNVYNDAGRETQFTFV"
     gene            complement(1767..3179)
                     /locus_tag="PK742_09040"
     CDS             complement(1767..3179)
                     /locus_tag="PK742_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76773.1"
                     /translation="MKLLLTFVFAVLLSGLQAQVDTVLLDEAGHFYFEDPFLPYSAYT
                     SDLDELDRPFIYSACLELGLVTFEYDASGNIWPVDTLVPSVFAGLKPTNVWQESNTLY
                     VSLGGYAGLIPQRAGLAVVDVSDPANMSLTAQWDSIAWNQGSAKVIKHGNTVFLGAME
                     RGVIAIDVSDPADPQWLSTFLPDPDFPDIPGLFSTPNARGLCMYGDDMLLVCYDHGGL
                     RMLDVSDPSNMTETGMYMNWDLYDVAAPAYNNVAVIGDYAYVPVDYCGLDVVYLAGDT
                     MSNAFWFNPWECTELNWDGAPGHANEVRVVGDSLLFISGGDSEVLVFDVTDPSTPVMK
                     GAYAYPGDSIVAWGMDVKDNLVSLALIDNSVLQVPYYSDKGGVMLLNMEVVIPADMQE
                     NHDLRIHLYPNPAYDRIHVSGLQTGSAFYSLRAMDGRILATGTIIDNGAISISALPSG
                     WYQLVLTMDQIASTITMLKI"
     gene            complement(3723..4142)
                     /locus_tag="PK742_09045"
     CDS             complement(3723..4142)
                     /locus_tag="PK742_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76774.1"
                     /translation="MRNVLLTLIISLAVLPASAQDQHIGTWQNNDNGDIGYVKFNAEG
                     YAWFIINNTPQGGPSFTLDGEQCKMTYTVNYDAVPHTVDFIITSLESGQVVEKQLGIF
                     AFKTDYQTMYLCLDLYRRNRPTTFEEDYTMVLARLWQ"
     gene            4320..>5695
                     /gene="purL"
                     /locus_tag="PK742_09050"
     CDS             4320..>5695
                     /gene="purL"
                     /locus_tag="PK742_09050"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HQU76775.1"
                     /translation="MTPNDQLPHPGVETAKELGLLAEEYEQICDILGREPNFTELSIF
                     SVMWSEHCSYKNSIRWLKTLPRSGAKMLVEAGEENAGLIDIGDGLACAFKIESHNHPS
                     AIEPYQGAATGVGGIHRDIFTMGARPIAALNSLRFGNPLKAHTRHLVRGVVKGIGDYG
                     NCFGVPTVGGEVYFEDCYQTNPLVNAMSVGILETGKMVSAIALGEGNPVYIVGSATGK
                     DGIHGASFASADLHEESHEDLPSVQVGDPFQEKLLLEASLEAIATGAVVGMQDMGAAG
                     ITCSTSEMSAKGEHGMIIHLDRVPTRQENMKSWEILLSESQERMLVVVEKGREKEIEA
                     VFEKWDLHAVVIGEVTTGGTLQYFMHDQLMAEVPAHSLVLGGGAPVYEREYREAAYYK
                     KLQSVNLDELPVPDDLPAIAKQIAARPNIASKRWIYTQYDSMVGIRNTSTNKASDAAV
                     VRITGTKKG"
BASE COUNT         1488 a         1369 c         1408 g         1430 t
ORIGIN      
        1 gacaaaggta aactgagttt cccggccggc atcgttatac acattgccta tatacaaacc
       61 agcaggaaat gaatcaagca ccacattggt ctgatcataa aatgaggata tatatatatc
      121 ttttcctgac tgatctgtta tccggatata ccaggttccc ggctccttgc aagacacatg
      181 aagcaaattg tttgccggat tgggatacca ttgtacaatc gtatttgctg acgtttcgat
      241 ctgatctgtg gtacatgagt cagcactaaa ctgaatgacc ctggttgacc atttagagct
      301 cgcattgtat tgagcagtga accagaaact tccatctgtc gggtcactca ccatactgtt
      361 gtaatcgccg tatgaattgg atgcatttgg tgcactgccg gttgcaccgg tgatctcttc
      421 acccatggtt ccctgtggat cgcaagggcg atgcgtggct gccctgaatc cggggaaaac
      481 cgtcgatgaa actatattgt aacccagtgc gatcactcca tcttcattga tgctgatact
      541 tcccatgaac cggtggtttt catcaggact ttgcagtcct tcctgaacca gtgaccagct
      601 gcccgatgca tcgcaaagga gttcatacca tttcattccg gcaacactgt cgcccgcatt
      661 taccacatgg gtgcatacca catgcagatt gccatccatg atgaccattt gtggcttgtc
      721 catcagaaaa tcctcacagg gccagatcct gacgtctgtg ccgggttgtt ccagacatgc
      781 atttggcgtg actgaatcac aaacactgtg gtcaaaagac ataacaggaa tgatgacagg
      841 acctgtgacc gtggccgcga gcgggtcagc ccagttgatc tggaaagaaa agatctccag
      901 acgatctgct acactgtctt ccgggtaata actgccttct acgatcctgg caacagatgg
      961 aggtcgtgca tccggtaatg gattgtaacc ggtttggtga ataggtgaca gaccctgcca
     1021 gctcataccg ggaattctga gaactgatag gctgaccaca tcgagggttt cttcgccgct
     1081 cagaagctga tctctgttga ccagatacac agcggggtgg ctggtggctg ttgtaataac
     1141 ataactgttg ccccaccttc ccagcttttc gtaatcgggc catttgccgg gagtgataaa
     1201 tgaataaccg gtccagctgc ctgttggatc attggaatca gataccgcta tgatgagttt
     1261 ctggtcttct tctgaaaaca aaaaggtgat cacaaaacgg tctgcttccc agtcatagat
     1321 gatgttggga tctccccagt caaaatcgcc ggtgaggtct ttgaacaaag atttatcgat
     1381 cagaatgttg ccggctttat cccacacacg tatcagactg ctcaggtagg aactgttaat
     1441 gacctgtaca acatgttccg gtcccaccgc aatgttgtta tctgatctga ggtaatctga
     1501 gtagatgcct tccaatgact gcagcagaga agatgatctc cgtagctccg gttcactgaa
     1561 atacacggaa tcatgggctg atgctgtcag ttcagtgaaa taatttcggt tcctgtcaat
     1621 gagcaactcc ttctcggtta cggcatgctg cagaatagag ccggttatct tttcggcctg
     1681 tatgagtaca ggaggttcct gtgcataaca aaggcaactg cttatcatga aagctgccaa
     1741 cagggttgtt ctcatatgcc gtaagtttat atttttaaca tagttatggt tgatgcaatc
     1801 tgatccattg tcagcaccag ttgataccat ccggaaggaa gtgcagaaat gctgatcgcc
     1861 ccgttgtcaa taatagtacc tgttgccaat atgcggccat ccattgctct cagtgaataa
     1921 aaagcgctac ctgtttgcag gccggaaaca tggatccggt catatgcagg attgggatac
     1981 aaatgtatcc ggagatcatg attttcctgc atgtcggcag gtatgaccac ttccatgttc
     2041 agcagcatca cacccccttt gtctgagtaa taaggaacct gtaatacact gttgtcaatc
     2101 agggcaagac tcacgaggtt gtctttcaca tccattcccc aggctacgat actatcgccc
     2161 ggataagcat aagccccttt cattaccggg gtggaagggt cggttacatc gaataccagc
     2221 acctcagaat caccacctga aatgaacagc agtgaatcgc ccactacccg cacttcattg
     2281 gcatgacccg gtgcgccatc ccagttaagt tcagtacact cccatggatt gaaccagaag
     2341 gcgttgctca tggtgtcgcc ggccagatag accacatcaa ggccacaata atctaccggc
     2401 acgtatgcat aatcaccaat gaccgccaca ttgttatagg caggtgccgc cacatcatac
     2461 aggtcccagt tcatatacat accggtctcg gtcatgttgg aaggatcact cacatccagc
     2521 atgcgcagtc cgccatgatc ataacaaacc agcaacatat catcaccata catgcacagt
     2581 ccgcgggcat tgggggtgct gaacaatccc ggaatatccg gaaaatccgg atcgggcagg
     2641 aaggtgctca gccactgcgg atcagcagga tcactaacgt ctatggcaat gacaccacgt
     2701 tccatggcac ccagaaaaac ggtgttgccg tgtttgatca ctttggccga tccctgattc
     2761 caggcgatac tgtcccattg tgctgtcaga ctcatattgg ctggatcgct cacatccacc
     2821 actgcaagtc ccgcccgctg aggtatcagc ccggcatagc cgcccagaga gacatacaaa
     2881 gtgttggatt cctgccagac gttcgtgggt ttgagtccgg caaatacgga aggtacaagt
     2941 gtatccaccg gccagatatt gcccgaagca tcatactcaa atgttaccag ccccagttcc
     3001 agacaggcac tgtatataaa aggtctgtcc agttcatcca gatctgaggt ataggcagag
     3061 tagggcagaa aggggtcttc aaaatagaaa tgtcccgctt catccagtag cacagtgtcc
     3121 acctgagcct gtagtcctga taaaagcacc gcaaaaacaa acgtgagcag tagtttcata
     3181 caataaattt atggaaaagt atcaataagc taatgagata ttttttattg ttttgtcatt
     3241 ggaatgctct cccaaagttt aaattcatag acaatctaaa aggatgttat ttggctggtt
     3301 tgacctgatc acgtatctga cgataatttc aattttcctg gggtttaaga acatcagatt
     3361 catgatcagt tttggtgttc tgatgtatgt agtgttattt gttattttac taccagccat
     3421 ttctgttagt attgaattac atcggataga agtaacttac ggaccttggt atgaatcatt
     3481 ggaagtgatg tatacgctat ttagattccc tatgtattgg ataacagggc tgttcatctt
     3541 aggtctgtac attcggcgtt ggcactatca ggacctgaag aaatcaggca aagaatagtt
     3601 gccgtgcggc aagtcgaatg ttttacaatg catattgccc caatgtatcc caatactttt
     3661 cagaagatgt gaactggcta ctgcagactg cccattacct cctgatggcc atcagaatgc
     3721 gctcactgcc acagcctcgc cagcaccatg gtatagtcct cttcaaaagt ggtgggtcgg
     3781 ttgcgccggt acaggtccag gcagaggtac atggtctggt agtctgtttt gaacgcaaag
     3841 atgccaagct gcttttccac tacctgtccg ctttccaggc tggtgatgat gaaatcaact
     3901 gtgtggggaa ccgcatcgta attgactgta taggtcatct tgcactgctc accatccagt
     3961 gtaaaggatg ggccgccttg tggcgtattg ttgatgatga accaggcata gccctccgca
     4021 ttgaacttga catagcctat atcgccgtta tcattgttct gccaggtgcc gatatgctgg
     4081 tcctgggcag aagccggtaa aactgccaga gaaatgatca gggtgaggag gacgttgcgc
     4141 atgcttgatg tttggtctga atactaaccc aaatttcagg ccgaaattac acaaacggtc
     4201 aggtttgccc cctgggttac ccacagacgc atctgatatg tgtaaactat gtgggagttg
     4261 ggtgctgata ggcctgtttt ctctgatagg tacagcatag aatcgtaatt ttgcagtcta
     4321 tgacaccgaa cgatcagttg ccccatcccg gggtggaaac ggccaaagaa ctcggactgc
     4381 tggctgaaga atacgaacag atctgcgata tcctcggaag agagcccaac ttcaccgaac
     4441 tgagcatctt cagcgtgatg tggagtgaac actgctctta caagaattcc atccgctggc
     4501 tgaagaccct tccccgttca ggtgccaaaa tgctggtaga agccggagaa gaaaatgcgg
     4561 ggctgatcga tataggcgat ggtctggcct gcgcttttaa gatagaaagt cataaccatc
     4621 ccagtgccat cgagccctat cagggtgctg ccacaggtgt gggtggtatc caccgtgaca
     4681 tcttcaccat gggagcaaga cccattgctg cgctgaattc gcttcgtttt ggcaatccgc
     4741 tgaaagcaca tacccgtcat ctggtgcgtg gagtggtaaa aggcatcggc gattatggca
     4801 attgctttgg cgtacccaca gttggagggg aagtttattt tgaagattgt tatcagacca
     4861 atccgctggt caatgccatg agcgtaggca ttctggagac cggtaaaatg gtttctgcca
     4921 tcgcactggg cgaaggtaat ccggtctata tagtaggttc tgccaccggc aaggatggta
     4981 ttcatggtgc ctcattcgca agtgccgatc tgcatgaaga atcgcatgaa gaccttccct
     5041 ctgtacaggt gggagatccc ttccaggaaa aactgctgct ggaagcttct ctcgaagcca
     5101 ttgccacagg agccgtggtg ggtatgcagg acatgggtgc ggcaggtatc acctgttcca
     5161 ccagtgagat gagtgcgaaa ggagaacacg gtatgatcat ccacctggac cgggtaccta
     5221 cacgccagga gaatatgaag agctgggaga tcctgctcag cgagagtcag gagcgtatgc
     5281 tggtggtagt ggagaaaggc cgggaaaagg agatcgaagc cgttttcgaa aaatgggatc
     5341 tgcatgcagt cgtcattggc gaagtgacca ccggaggaac attgcagtat ttcatgcacg
     5401 accagctgat ggcggaagtt cctgcacaca gcctggtgct gggtggtggt gctccggtat
     5461 atgaaagaga atacagggaa gcagcctact acaagaaatt gcagtcggtc aacctggacg
     5521 aattgcctgt acccgatgac cttccggcca tagcaaaaca gatcgccgca agaccgaata
     5581 tcgccagcaa gagatggatc tacacccagt acgacagcat ggtgggtatc agaaatactt
     5641 ctaccaacaa agcttctgac gccgcagtgg tcaggatcac cggtacgaaa aaagg
//
LOCUS       DAOMAX010000171         5649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2345_length_5649_cov_4.407043, whole genome shotgun sequence.
ACCESSION   DAOMAX010000171 DAOMAX010000000
VERSION     DAOMAX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2345_length_5649_cov_4.407043"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="cysN"
                     /locus_tag="PK742_09055"
     CDS             complement(<1..1290)
                     /gene="cysN"
                     /locus_tag="PK742_09055"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HQU76776.1"
                     /translation="MEHLIKDGTFDHHGYLNMELLRFTTAGSVDDGKSTLIGRLLYDS
                     KSIFQDQMEAIEKTSEQRGEEYVNLALLTDGLKAEREQGITIDVAYRYFATPKRKFII
                     ADTPGHIQYTRNMVTGASTANLAIILVDARNGVVEQTYRHAFIASLLQIPHIVICINK
                     MDLVDYSQEAYDKVVEQFKHFSSKLDVQDVRFIPISALKGDNVVERSETMSWYEGPTL
                     LYTLETVHIGGDHNLIDVRFPVQYVVRPMSDEYHDYRGYAGRVSGGVMKPGDTVMHLP
                     SGLTSKIAAIKTIDGDLDEAYPPQSVTVLLEDDIDISRGDMLVRENNIPDQGQDIDIM
                     LCWMSEKNGLQPGGKYAIKHTTRDARCVVKEILYKVDVNTLHRIQDVNTLTLNEIGRV
                     RIRTTVPLFYDEYRQNRETGSIIIIDEGTNNTVAAGMI"
     gene            complement(1315..2220)
                     /gene="cysD"
                     /locus_tag="PK742_09060"
     CDS             complement(1315..2220)
                     /gene="cysD"
                     /locus_tag="PK742_09060"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQU76777.1"
                     /translation="MSYSLTHLRELEAESIFVMREVAAQFQNPALLFSGGKDSILMVY
                     LARKAFWPARIPFPLVHIDTGHNFPETIEYRDWLVNEMGARLIVGHVEDSIARGTAVE
                     ERGINASRNALQTVTLLETVEAHKFDAAMGGARRDEEKARAKERFFSHRDEFGQWDPK
                     NQRPELWNLFNGKKHIGEHFRVFPISNWTEMDVWQYILLENIPLPKLYFAHEREVIER
                     DGTFISTHPTWVKLRENETVSKRMIRFRTCGDLPITGAIESDADTVEKIIYEVATTRV
                     TERGNRADDKRSETSMEDRKKQGYF"
     gene            complement(2228..2836)
                     /gene="cysC"
                     /locus_tag="PK742_09065"
     CDS             complement(2228..2836)
                     /gene="cysC"
                     /locus_tag="PK742_09065"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HQU76778.1"
                     /translation="MSVQEHIHPIFDRILQRSDKEALLRQRAKVIWLTGLSGSGKSTI
                     AQHIEKELHQRGLLTMLLDGDNIRSGINNNLSFSDTDRIENIRRIAEVSKLFLNCGII
                     TLNSFVSPTNEIREMARNIIGAEDFLEVYINAPLEVCEARDVKGLYAKARKGLIPDFT
                     GITAPFEAPEHPALEIRTDQMSVKESVRMLLDFILPIIQQQA"
     gene            complement(3099..4274)
                     /locus_tag="PK742_09070"
     CDS             complement(3099..4274)
                     /locus_tag="PK742_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQU76779.1"
                     /translation="MDVNIILIALAVTILGSYLFDLIGKRLKIPSVVLLIGTGILLGE
                     LARYVGFSIPYAETFLSTFGTVGLILIVLEGAMDLNLTRDKLPLIGKTLMAAIFIMLA
                     CVAGISWLLIYYFEVEFQQAVINAIPLSIISSAVAIPAASSLMKADKEFVVYESSFSD
                     IIGIVLFNYFTVNSEISVSTGFYELGSMAMIILVAVLLSLLVGYFIDKVTHHVKFVPI
                     LAFLMLAYGFGKYYHLSPLLLILIFGLILNNFVLLLRGPLVDLVNIVKMRTDMTQFKQ
                     LLAEAVFFARTFFFILFGYEADIYQMAGQETLMLGGIIFGIIVVTRLAGLLIIDRKSV
                     NPMIWFAPRGLITILLFLSIPEQYRMEMVNEGLLLIVIFLSLVLLILGSRLARQQRS"
     gene            4379..4455
                     /locus_tag="PK742_09075"
     tRNA            4379..4455
                     /locus_tag="PK742_09075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4413..4415,aa:Pro,seq:cgg)
     assembly_gap    4492..4591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4948..5490
                     /locus_tag="PK742_09080"
     CDS             4948..5490
                     /locus_tag="PK742_09080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HQU76780.1"
                     /translation="MPKKYPKVKVKKSDTGLGLFAAEDIKKNKIIIEYTGERISMKEA
                     NKRGGRYLFGVTKKIVIDAKDRKHTARYINHACKPNAEAEHDTTEDRIFIRSLRKIKE
                     GEEITYDYGKEYLEDLIGKKNCLCRHCVEKREKKEKKSEKEKKVNRRKLNAEKTKGTE
                     SGAEKTEAVVNDVTALPTDQ"
BASE COUNT         1384 a         1407 c         1398 g         1360 t
ORIGIN      
        1 gatcataccg gcggctaccg tgttgttggt gccttcatca atgatgatga tggaaccggt
       61 ctcgcggttc tggcggtatt catcgtagaa gagtggcacg gtggttcgga tgcgcaccct
      121 gcctatctcg ttcagtgtaa gggtattcac atcctggata cgatgcaggg tgttcacgtc
      181 cactttgtag aggatctctt tcactacaca acgggcatcg cgggtggtat gcttaatggc
      241 atattttcct cccggctgca ggccgttctt ctcgctcatc cagcacagca tgatgtctat
      301 atcctgaccc tgatcgggaa tgttgttctc tctgaccagc atatctccgc gactgatatc
      361 gatatcgtct tccagtagca cggtcacgct ttgaggcggg tatgcttcgt ccagatctcc
      421 atcaatggtt ttgatggcag cgatcttgct ggtaagacca gaaggcaggt gcataacggt
      481 atctccgggt ttcatcacac caccggaaac acgacctgca taaccgcggt agtcgtggta
      541 ttcatcgctc attgggcgaa ccacatactg taccggaaaa cgcacgtcga taaggttatg
      601 atcgccacca atgtgcacgg tctccagggt gtacagcaag gttggacctt cataccagct
      661 catggtctcg ctgcgttcca ccacattatc gcctttcagg gcgctgatgg gaatgaagcg
      721 cacgtcctgc acgtccagtt tggaagagaa atgcttgaat tgctctacca ctttatcgta
      781 agcttcctgg ctgtaatcaa ccaggtccat cttgttgata cagatcacga tgtgcgggat
      841 ctgcagcagc gatgcgatga atgcatggcg ataggtctgt tccaccaccc catttctggc
      901 atccaccaga atgatggcga ggttagctgt agaagcaccg gtcaccatgt tgcgggtgta
      961 ctgaatatga cccggtgtat ctgcgatgat gaacttacgt ttgggcgtag cgaagtagcg
     1021 gtaggccaca tcaatggtga tgccctgctc ccgttctgct ttcagaccat cggtcagcag
     1081 agcgaggttc acgtattctt cacctctttg ttctgaggtt ttttcaatgg cctccatctg
     1141 gtcctggaaa atggacttgc tgtcatacaa caaccgacct atcagggtcg attttccatc
     1201 atccacacta cccgctgtgg taaagcggag cagttccata ttgagataac catggtggtc
     1261 aaaagtgccg tctttgatca aatgttccat actggctgta tcgtttcccg atgtttagaa
     1321 atatccttgt ttcttcctgt cttccataga agtttcgctc cgcttatcgt cggcccggtt
     1381 accacgttct gtgaccctgg tcgtggccac ttcatagatg atcttctcaa cggtgtctgc
     1441 atcgctttca atagctccgg taatgggcag gtcaccgcag gtgcggaagc ggatcatgcg
     1501 tttgctcacg gtctcattct cacgcagttt cacccaggtg ggatgcgtgg agatgaaggt
     1561 gccgtctctt tcgatgacct ctctttcatg tgcgaagtag agtttaggca gcggaatgtt
     1621 ttccagcagg atgtactgcc agacgtccat ttcggtccag ttggagatgg ggaaaacccg
     1681 gaagtgttcg cctatatgtt tcttgccgtt gaacaggttc cagagttccg gacgctggtt
     1741 cttcggatcc cactggccga actcatcacg gtggctgaaa aatctttctt tggcacgggc
     1801 cttttcttca tcacggcggg caccacccat ggcagcatca aacttatgtg cttctactgt
     1861 ttccagcagg gttacagttt gcagggcatt gcgactggca ttgatccctc tttcttccac
     1921 agctgtaccg cgggcgatac tgtcttccac atgccctacg atcaaacgtg cacccatctc
     1981 attcacgagc cagtcgcgat actcgatggt ttcagggaag ttgtgtccgg tatcaatgtg
     2041 taccagcggg aaggggatgc gggccggcca gaatgctttt cgtgccaggt acaccatgag
     2101 aatggaatcc tttccgccgg agaacagcag tgcaggattc tgaaactggg cggctacttc
     2161 ccgcatcacg aagatgcttt cggcttccag ttcacgtaaa tgggtcagac tataagacat
     2221 gattcggtta tgcttgttgt tggatgattg gcaggatgaa atcgagcagc atgcgcacac
     2281 tttctttcac agacatctgg tctgtgcgga tctccagggc aggatgttca ggtgcttcaa
     2341 aaggagcggt gattccggta aaatcaggga tcagtccttt tcttgccttg gcatacaatc
     2401 ctttcacatc gcgggcctca cagacctcaa ggggtgcgtt gatatagacc tccaggaaat
     2461 cttcagcacc aatgatgtta cgtgccattt ctctgatctc gttggtgggg cttacaaagc
     2521 tgttcagggt gatgatgccg cagttcagaa agagcttgct gacctctgct atgcggcgga
     2581 tgttctctat tcgatcagta tcgctgaagc tcaggttgtt gttgataccg gagcggatat
     2641 tatcgccatc cagcaacata gtgagcagtc cacgctggtg cagctccttc tctatatgct
     2701 gggcaatggt actctttcct gagcctgaca gtccggtcag ccagatcact ttggcacgtt
     2761 gtctcaacag cgcttcttta tcagaccgct gcaggatcct gtcaaaaatg gggtggatat
     2821 gctcctgaac actcataatt ggcgccgcaa aggtacaaaa ccttcgtcag ttacttgaca
     2881 gtcaaatgcc tttcggggtt gtatctgccg gcatttttcc ccgatctgat acgttattgc
     2941 cttcgttgtg gtctttccaa ccactgtctc cagatctttg cagttaccct tcaggatggc
     3001 cggagaacca aattcatggc gttattgcag aaaattgagg ttcgcagatg ttaccgccat
     3061 catgtcagac aggaaagcag ctaaccggca gttacagctc aggatcgctg ctgcctggcc
     3121 aggcgactac ccagtatcag caggaccagc gacaggaaga tcacgatgag cagcagccct
     3181 tcattgacca tctccatcct gtattgctca gggatactga ggaagaggag gatggtgatc
     3241 aacccgcggg gagcgaacca gatcatcggg ttgaccgact tccggtcgat gatgagcagg
     3301 ccggccagac gtgtcaccac gatgatgccg aatatgatac cccccagcat cagggtctcc
     3361 tgtcctgcca tctggtagat atcagcttca tatccgaaca gaatgaagaa aaaggtgcgt
     3421 gcaaagaaaa ccgcttctgc caacagttgc ttgaactggg tcatatcagt gcgcatcttg
     3481 acgatgttca ccaggtccac aagcggcccg cgtaacaaca ggacgaaatt gttcaggatg
     3541 agaccaaaga tgaggatgag cagcaggggc gataaatggt agtatttacc aaatccatat
     3601 gccagcatga ggaaggccag gatgggaacg aattttacat ggtgggtcac tttgtcgatg
     3661 aagtatccca ccagcaacga gagcagtact gcaaccagga tgatcatagc catgctgccc
     3721 agttcataaa aaccggttga gacactgatc tcgctgttca cagtgaaata attgaacagt
     3781 acgataccga tgatatcaga gaacgaactc tcatagacca cgaattcctt atcggctttc
     3841 atcaacgaac tggctgccgg aatggccact gcactactga tgatagacaa aggaatggca
     3901 ttgatgacgg cctgctgaaa ttccacttca aaataataga tgagcaacca gctgatgcct
     3961 gccacacacg ccagcatgat gaagatggcg gccatgaggg tcttgccgat gagtggaagt
     4021 ttgtctctgg tcaggttcag gtccatggca ccttccagca cgatgaggat cagaccaact
     4081 gttccgaaag ttgacaggaa ggtttcagca taggggatac tgaacccaac atacctggcc
     4141 agttcgccta acaggatgcc ggtacctata agcagtacca cagagggaat cttcagccgc
     4201 ttgccgatca ggtcaaacag gtaggaaccc agaatggtga ccgccagtgc gatcaatatg
     4261 atattgacat ccatgttatg caaaaatggg caaaacgata ttaatggagt tcaccctgct
     4321 tccattttat catccgcctt aactgcgggt tggatttttg gtaattttgt gccccacccg
     4381 gggtgtagct cagcttggca gagcgctacg ttcgggacgt agaggccggt ggttcgaatc
     4441 cactcacccc gaccaaaaag tttgcccctg acgatagtca ggggattttt tnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggcagacc acattgagta caccccactt
     4621 tgtggatcga tgaaatcaat ccactcaccc cgaccaaaaa gtttgcccct gacgatagtc
     4681 aggggatttt ttatgccacg gcgaaccagg ttgaaaacct gagcgagcag gggcatgaaa
     4741 aatcttctgg cggctgcaag ccgacagggc agaccacatt gagtacaccc cactttgtgg
     4801 atcgatgaaa tcaatccact ctcactccac tcccccaccc actcccactc ccactctcac
     4861 tcccactccc actctcactc ccactccccc acccactccc actactctgt acccgatact
     4921 aagaatatcc gtaatatagc aacccctatg cccaagaaat atcccaaggt taaggtcaag
     4981 aagtcagaca ccggactcgg actgttcgct gcagaagaca tcaagaaaaa caagatcatc
     5041 attgaatata caggagagcg tatttccatg aaggaagcca acaaaagagg tgggcgctat
     5101 ctgttcgggg tgacaaaaaa aattgtcatt gatgccaaag atcgcaagca tacggcccgg
     5161 tacatcaacc atgcctgtaa gcccaatgca gaggctgaac acgacactac agaagaccgc
     5221 atattcattc gctcacttcg caagatcaaa gaaggtgaag agatcaccta tgactatggc
     5281 aaagaatacc tggaagacct gatcggtaaa aagaattgtc tgtgccgcca ctgcgtggag
     5341 aagagggaga aaaaagagaa gaaaagtgaa aaagagaaga aagttaacag gagaaagtta
     5401 aacgcagaaa agaccaaagg gacagaaagt ggggcagaaa agacagaagc agtggtgaat
     5461 gacgttactg cgctgcccac tgatcagtga gcagcagtat ctatatctat ttactaaaaa
     5521 acaaccgcca caccttgcgg tgcggcggtt tgtatcgtcg gggtggcagg attacgtccg
     5581 cgtgatcctg gtagtggggt gtactgatcc gaaccgttgt tgcacttcgt gcacccgggt
     5641 tgttttttt
//
LOCUS       DAOMAX010000172         5640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2354_length_5640_cov_4.830439, whole genome shotgun sequence.
ACCESSION   DAOMAX010000172 DAOMAX010000000
VERSION     DAOMAX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_5640_cov_4.830439"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PK742_09085"
     CDS             complement(<1..764)
                     /locus_tag="PK742_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76781.1"
                     /translation="MRKLKTLFLAGLCSVGILSAQTYDVLFIVDMNEVADPFTTPEVN
                     GTFNVWCGGCAPMSDDNGDNIWELTIPLDAGTYEYKFAYDTWTGQESLTPGDECTLTT
                     DIFTNRLLVVSEDMELDTVCWGSCDACEDVLPSYTVTFRVDMSQVTDTYTTPEVNGTF
                     NDWCGGCAPMADDDADDIWVLDIVLEQGSYEYKFAYDAWAGQEELTEGDPCTVTTDAF
                     TNRALDVSSDTVLTAVCWGSCDECVTIPEAIENVTS"
     gene            complement(812..3271)
                     /locus_tag="PK742_09090"
     CDS             complement(812..3271)
                     /locus_tag="PK742_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76782.1"
                     /translation="MKRIIQLVVIALLMSSAAMAQTYNITFRVDMNEVGDAFTTPEVN
                     GTFNGWCGGCAPMSDGDGDGIWELTIPLAAGFYEYKFAYDSWAGSESLIPGSSCTITT
                     GEFTNRALDVTGDATLDAVCWGSCLSCSDIITYAVTFSVDMNEVAGPFTTPEVNGSWD
                     GWCGGCTPLSDPDGDGIWSTTKMLEPGYYEYKFAYDSWSGDESLTPGDPCTVTTDIFT
                     NRFINVSDDADLGVVCWASCEACETIGLEQMDLPVTFEDAGVDYGVIGFEGAEASSIV
                     TDPTDASNTVVQVIKSASAMPWAGTTVTNAAEEGFATPIPFTAENTQMTVRVWSPDAG
                     IQVRLKVEDHLDPTHSVETEATSTVAGDWETLTFNFSNEAPGTAELNLAYTFDKASIF
                     FNFGVDGATAGEKTYYFDDIYFAEGGGASSYTVTFWVDMNEVLAPFTTPEVNGSWDGW
                     CGGCTPMEDPDGDNIWTATKVLDAGYYEYKFAYDSWTGSETLTPGDPCTVTTDIFTNR
                     FINVSEDTELGVVCWESCEACTGEEPVMITFSVNMNTVADPFTTPEVNGSWDGWCGGC
                     TPMEDPDGDGIWTATKMLSPGYYEYKFAYDSWTGQENLTPGDPCTVTTDIFTNRFIDA
                     VADEVLPVVCWESCENCAEDCNAPVDVVVTDITSSGATISWTGTSGADQYLITLADFA
                     AGTSGKQRTGPATTFVIEDMLAPSTEYSIRIKSICYDLGLRSEPSETVYFTTAPMRSG
                     GEFHSAVYPNPTAGNLQVQTNLSGTVMLTVTGMTGQLLMSTIIEAGSTSLDLSMFADG
                     LYMIRLDNGQSSEIFTVSLNR"
     gene            complement(3320..4915)
                     /locus_tag="PK742_09095"
     CDS             complement(3320..4915)
                     /locus_tag="PK742_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76783.1"
                     /translation="MRSIFTFFAAVLLSTAAMAQTYNVTFRVDMNEVGDPYTTPEVNG
                     SWDGWCGGCTPLEDPDGDGVWTITKTLDAGFYEYKFAFDSWAGSETLAPGSSCTITTG
                     GFTNRFVDVTGDVALDTVCYGACTTCSEAVTYDITFYVNMNEVGDPYTTPEVNGNWDG
                     WCGGCTPLEDPDGDGIWSITKSLSPGTYEYKFAYDSWTGSENLAPGTDCTITTGGFTN
                     RIITVDADAALPVVCWGSCSNCGEAVLYDVTFQVNMNEVADPFTTPEVNGTFNGWCGG
                     CAPMSDGDGDGIWEITIPLEAGTYEYKFAYDSWAGQETLTPGDPCTMTTDVFTNRVIT
                     ISEETVIGAVCWGSCDNCVAATCELPSDVTIDYVGGTGTEATVTWTAVPGATQYGLRY
                     RELGTPEWLKKATTGTGVFLIGLTPGATYQYQMVSRCGEDRAGPGSIATFTTPTRMGQ
                     FGSDDWMVYPNPSNGTFQLVSSGLSEQAMLMVMNVAGQQVYETMITNDSQTITLNGLA
                     DGLYTLVIVAGESMSATTISILQ"
     gene            5086..>5640
                     /locus_tag="PK742_09100"
     CDS             5086..>5640
                     /locus_tag="PK742_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQU76784.1"
                     /translation="MKNNFDNANRRKKPQDNDRMMEVSIDCVVFGYGVQSLEVMLIEI
                     GAEELQGSWALPGDLVYPQEDIDEAAHRVLRDLTGLAHIPLQQAHAFGEADRVPGRRV
                     VTIGYYAIVNKLDVQPQPSHWANKAQWFSLDDIPELTFDHADIIRYALDKLRHRTQHS
                     TRRRPLWEAVLPQFFTLSELQQLYD"
BASE COUNT         1476 a         1421 c         1372 g         1371 t
ORIGIN      
        1 tttgacgtaa cattttcaat ggcctcaggt attgtcacac attcatcgca ggagccccag
       61 caaactgctg tgagaaccgt atcagaacta acgtcaagcg ccctgttggt gaatgcatcg
      121 gttgtaacgg tacaaggatc accttcggtc agttcctcct gtccggccca ggcatcgtag
      181 gcaaacttgt attcatagga gccttgttcc agcacgatat ccagcaccca tatatcatca
      241 gcatcatcgt ctgccatagg agcacaacca ccgcaccagt cattgaatgt tccgtttacc
      301 tcaggtgtcg tataggtatc agttacctga ctcatatcta ctctgaaggt aaccgtatag
      361 gagggcagta catcttcaca agcatcgcag cttccccagc aaacagtatc cagttccatg
      421 tcttcagaca caaccagcaa tcggttggtg aagatatctg tggtcagggt gcactcatca
      481 ccgggtgtaa gtgattcctg tccggtccag gtgtcatagg cgaatttata ttcatatgta
      541 cctgcatcca gggggatggt cagttcccag atattgtctc cgttatcatc actcatagga
      601 gcacaacctc cacaccagac gttgaaagtg ccattcactt caggtgtggt gaacggatct
      661 gctacttcat tcatatcaac aatgaagagc acatcatagg tctgtgctga caatatgcca
      721 acagaacaca gacctgccag aaataatgtt ttaagttttc tcatatatat gtgtttaaaa
      781 attcgggggt gtcaggcacc cccgaattta cttatctgtt cagtgaaacg gtaaagatct
      841 ctgaagactg tccgttatcc agacggatca tatacagacc atctgcaaac atgctcagat
      901 caagagaagt gctaccggct tcgataatgg tgctcatcag caactgaccg gtcattcctg
      961 ttacggtcag catgacagtg ccgctgaggt tggtttgtac ctgcagattt ccagcagtag
     1021 ggttcggata cactgcagag tgaaattcgc cacctgaacg cataggtgcg gtggtgaaat
     1081 acacggtttc tgaaggttct gaacgcaaac cgaggtcgta gcagatagat ttgatacgaa
     1141 tagaatattc agtggaagga gccagcatat cctctattac aaaggtagtt gcaggacctg
     1201 tgcgttgttt accgctggta ccagctgcaa aatcagccag ggtgatcagg tactgatcag
     1261 caccggatgt tccggtccag gaaatggtag cccctgaaga ggtgatatct gttaccacta
     1321 catcaaccgg tgcgttacaa tcttctgcac agttctcaca gctttcccag caaaccactg
     1381 gcagcacttc atcagcaact gcatcaatga agcggttggt gaagatatct gtggtcacag
     1441 tacagggatc gccgggggtc aggttttcct gtcctgtcca gctgtcataa gcaaacttgt
     1501 attcgtagta accgggagaa agcattttgg ttgcggtcca gataccgtcg ccatcaggat
     1561 cttccatcgg ggtacaaccg ccgcaccagc catcccagct gccgttcact tcaggtgtag
     1621 tgaaaggatc tgccactgta ttcatgttca cagagaaggt gatcatgacc ggttcttctc
     1681 cggtacaggc ttcgcagctt tcccagcaaa ccacgcccag ttcggtatct tctgacacat
     1741 tgataaaccg gttggtaaag atatcggtgg tcacggtaca gggatcgccg ggagtcaggg
     1801 tttcacttcc tgtccagctg tcataggcaa acttgtactc ataataacct gcatccagca
     1861 ccttggtagc ggtccagata ttgtcgccgt caggatcttc catcggggta caaccaccac
     1921 accagccatc ccagctgccg ttcacttcag gagtggtaaa aggtgccagc acctcattca
     1981 tatccaccca gaaagtaaca gtataggaag aagcgccacc gccttctgca aaatagatgt
     2041 catcaaaata gtaggtcttt tcacctgcag tagctccatc tacgccaaag ttgaagaaga
     2101 tggaagcctt gtcaaaagta taagccaggt tgagttctgc tgtaccggga gcttcattgc
     2161 tgaagttaaa ggtcagcgtt tcccagtcgc cggcaacggt gctggttgct tcggtctcta
     2221 cagagtgggt aggatccagg tgatcttcca ctttcagacg tacctgaata cctgcatcgg
     2281 gactccagac ccgaacggtc atctgtgtat tttctgcggt aaaaggaatc ggtgtagcga
     2341 atccttcttc agcggcattg gttacggtag tacctgccca gggcatagcg gaagcagatt
     2401 tgatgacctg cacgactgtg ttggaagcat cggtaggatc ggttacaatg gaagaagctt
     2461 cagcgccttc aaatccgatg acaccgtaat ctacaccagc atcttcgaat gttacaggaa
     2521 ggtccatttg ttcgaggcca atggtttcac aagcctcaca gctggcccag cataccacgc
     2581 ccagatctgc atcatcagac acgttgatga atctgttggt gaaaatgtct gtagtaactg
     2641 tacaaggatc gccgggggtc agagactcat caccagacca gctgtcgtat gcaaacttat
     2701 attcatagta accgggttcc agcattttgg tagtggacca gataccgtcg ccatcaggat
     2761 cagacagcgg ggtacaacca ccgcaccatc cgtcccagct accgttcact tcaggtgtag
     2821 tgaaagggcc ggccacttcg ttcatatcta ctgaaaaagt taccgcataa gtgatgatat
     2881 ctgaacagct caggcagctt ccccagcaaa ccgcatcgag ggtagcatcg ccggttacgt
     2941 ccagtgcacg gttggtgaat tcacctgtgg tgatggtaca ggaactaccc gggatcagag
     3001 actcagagcc tgcccaggaa tcataagcaa atttgtattc ataaaatccg gctgcgagtg
     3061 gaatggtgag ttcccagata ccgtcgccat cgccgtcaga cattggcgca cagccaccgc
     3121 accagccatt gaaggtgccg ttcacctccg gtgtggtgaa agcatcacct acttcattca
     3181 tatccacccg gaaggtgata ttgtaggttt gggccatcgc tgccgaagac atcagcagtg
     3241 cgataaccac gagttgaata atacgtttca tttgggtagt gtttaaaggg gagctgattg
     3301 tcagctcccc ggatgaaatt tattgcagaa tggaaatggt agttgcagac atactttcac
     3361 ctgcaacgat taccagggtg tacaagccat cggcaaggcc attcagggtt atggtctggc
     3421 tgtcgttggt gatcatggtc tcatacacct gctgacctgc cacattcatg accatcagca
     3481 tggcttgttc tgacagacct gaagaaacca gctggaaggt accattgctt ggattaggat
     3541 ataccatcca gtcatcagaa ccaaactgtc ccattctggt aggagtagtg aaggtagcaa
     3601 tggaaccggg gcctgcacgg tcttcaccgc agcggcttac catctgatac tgatatgttg
     3661 caccaggggt caggccgatc aggaaaacac cggtacctgt ggtagctttt ttaagccatt
     3721 caggagtacc aagttcacga taacggagtc cgtactgggt agcaccaggt acagcggtcc
     3781 aggtaacggt tgcttccgtg ccggtacctc ctacatagtc aatggtcaca tcagacggaa
     3841 gctcgcaggt agctgcaaca caattgtcgc agctgcccca gcatactgca cctatcacag
     3901 tttcttcgct gatggtgatc accctgttgg tgaatacatc agtggtcata gtacaaggat
     3961 cgccgggagt cagggtttcc tgtcctgccc agctatcata tgcaaattta tattcatacg
     4021 tgccggcttc cagaggaatg gtgatctccc agataccgtc gccatcgcca tctgacatag
     4081 gcgcacaacc gccgcaccaa ccattgaaag taccattcac ttctggtgtg gtgaacggat
     4141 cagccacttc attcatgttg acctggaagg ttacatcata cagtacagcc tctccgcagt
     4201 tgctgcaact tccccagcat actaccggga gggcagcatc tgcatctaca gttataatcc
     4261 ggttggtaaa gcctccggtg gtgatggtac agtctgtacc gggggcgagg ttctctgaac
     4321 ctgtccagct gtcataggca aacttgtatt cataggtacc gggagagaga gacttggtta
     4381 tactccagat accatctccg tcaggatctt ccagcggagt acaaccaccg caccagccgt
     4441 cccagttacc attcacttca ggagtggtat aaggatcgcc tacttcattc atgttcacat
     4501 aaaaagtgat gtcatatgtg acggcttctg aacaggtagt acaggcacca taacatacgg
     4561 tatcgagggc aacatcgccg gtcacatcaa caaagcggtt ggtaaagcca ccggtggtaa
     4621 tggtacagga tgagcccgga gccagggtct cagatccggc ccagctgtca aaggcaaact
     4681 tatactcata aaagcctgca tccagtgttt tggtaatggt ccacacacca tcaccatcag
     4741 gatcttccag cggagtacaa ccgccacacc agccatccca gctgccgttt acttcaggag
     4801 ttgtgtaagg gtcacctact tcattcatgt caacccggaa ggtgacattg taggtctgag
     4861 ccattgcggc tgtggagagc agaacagctg caaagaaggt aaaaatggat ctcattggta
     4921 ggaattttaa aaggttatag ctgctattta ctcagtaaaa atatcccttt tagtataaaa
     4981 tacaaaaagt tggaaaaaga atttgtaatt tttacaaaaa gctacctatt ttagctaaac
     5041 aggactttct gcagcacatt tgtagaaaga ttcattcaac aacagatgaa aaacaacttc
     5101 gataatgcca acaggaggaa gaagcctcag gacaatgacc gcatgatgga ggtttccatt
     5161 gactgtgtag tgtttggcta cggggtacag tcactggagg tcatgctcat agaaataggt
     5221 gctgaagagc ttcaggggtc ctgggcactt ccgggagatc ttgtctatcc tcaggaagac
     5281 attgatgaag cagcccatcg tgtactgcga gacctgaccg gacttgctca tattcctctg
     5341 cagcaggcac acgccttcgg agaagctgac agggtgccgg gaagacgggt ggtaaccatt
     5401 ggatattacg caattgtcaa caaactggat gtgcagccgc agccgtcaca ctgggccaat
     5461 aaagctcaat ggttttcgct tgatgacatt ccggaactga cctttgacca tgcagacatc
     5521 attcgctacg ccctggataa actaagacac cgcacgcagc acagcacccg gcgcaggccg
     5581 ctgtgggaag ctgtgctgcc tcagttcttc acgctttcag aattgcagca actctacgac
//
LOCUS       DAOMAX010000173         5582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2387_length_5582_cov_4.843857, whole genome shotgun sequence.
ACCESSION   DAOMAX010000173 DAOMAX010000000
VERSION     DAOMAX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2387_length_5582_cov_4.843857"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK742_09105"
     CDS             <1..454
                     /locus_tag="PK742_09105"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HQU76785.1"
                     /translation="ISDFRLEAESPYPIPDREELRRKNMQLKYPKENVHFRHYGRYVE
                     SMIKQAISMEDPEKQEAFTEVIGNYMKLVYQNWNRENVTDDLIKSDLEFLSGGKLTLH
                     EESNLDTLSKANRTKQGPNQQQTQERRQGGRYQRNFKQNKNRNFKRNR"
     gene            454..1761
                     /gene="murA"
                     /locus_tag="PK742_09110"
     CDS             454..1761
                     /gene="murA"
                     /locus_tag="PK742_09110"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQU76786.1"
                     /translation="MGAFEIKGGKSLSGEIIPQGAKNEALQVICAVLLTPESVTITNI
                     PDILDVNLLIDLLGDMGVVIDRPSSDTCTFTARDVNLDFLQTEAFKEKSRRLRGSVMI
                     LGPLLARFKKASLPKPGGDKIGRRRLDTHFIGFEKLGARFNYDTHDAFYTIDASRLHG
                     TYMLLDEASVTGTANVVMAAVLAEGTTTIYNAACEPYLQQLCKMLVGMGAKISGIGSN
                     MLIIEGVEALHGTDHRMLPDMIEIGSFIGMAAMTQSEITIKDVAYDQLGLIPDVFSRL
                     GIQMERRGEDLYIPAQEHYEIQTFIDGSIMTIADAPWPGFTPDLLSIVLVVATQAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLDRRNQLRGLEMSSPDIRAGV
                     ALLIAALSAKGTSIIRNIEQIDRGYQRIDSRLQALGASIERIA"
     gene            1837..2385
                     /locus_tag="PK742_09115"
     CDS             1837..2385
                     /locus_tag="PK742_09115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQU76787.1"
                     /translation="MQYRSSLSLALTVFSLFFILTSSFTCSGSKEAVIGIKTGNLAPE
                     ILCDGMKGDCASLSSLEGKLVLVEFWTAASNICRQAHFEMGRMYNTFKDQKFENADGF
                     TIYSVSLDNSEEQWLQAIKEDKCNWPYQMCDPRKWNAPSVLTYEVNTLPKYFLIDGNG
                     EILYRYVEIADLENLLEDLSEH"
     gene            2472..3026
                     /locus_tag="PK742_09120"
     CDS             2472..3026
                     /locus_tag="PK742_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQU76788.1"
                     /translation="MSKKQEIQDEETTLSDTENTEQERPAEGMPVEEELVEESSSLVN
                     ELEQQKEKFIRLLAEFENYKRRTARERLDIIKTAGQDIMADLLPVLDDVDRASEIIDK
                     AQDVEAIRQGLVLISEKLKNMVAQKGLQPMEVKGQDFDAETMEAVTEIPAGDDMKGKV
                     VDVIEKGYTLNEKIIRYAKVVVGK"
     gene            3032..4198
                     /gene="dnaJ"
                     /locus_tag="PK742_09125"
     CDS             3032..4198
                     /gene="dnaJ"
                     /locus_tag="PK742_09125"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQU76789.1"
                     /translation="MAKRDYYEVLGVSKNASAEEIKKAYRKMALQYHPDKNPGNKEAE
                     EKFKEAAEAYDVLSNAEKKQRYDQFGHAGMGGAGGFGGAGGGMNMDDIFSQFGDIFGD
                     SIFDSFFGGGGRGRGAQRGRGKRGSNIRIKVKMTLDEIADGTKKTIKVKKYIPCDSCS
                     GTGAKDGSAYETCKTCGGQGAVRRVANTILGQMQTTQTCPTCNGSGQTIIANCGKCHG
                     EGRVYGEDTITLDIPAGVTDGVELSMSGRGNAGEQGGPNGDLLISVEEIPHSDLKREG
                     QHVLYNLYLNIADMALGTQVEVPTIGGRARIKIPEGTQSGHIMRLKGKGLPSLNSYGK
                     GDQIIQVNVWVPKHMTTEEKKTMEKLRDAKNFQPDEGETRKEKSFVDKIRGMFN"
     gene            4603..>5582
                     /locus_tag="PK742_09130"
     CDS             4603..>5582
                     /locus_tag="PK742_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76790.1"
                     /translation="MKTKLILTTVLLFAGLISFAQMGFGKEKEIVEVYERKVIIVNEQ
                     YDERTLKVLEKKGKKGDITTYKNVITKYNADLLEAVKTAWPFDTVIVVKKIEDVLEMK
                     KDKKYNYVVVRTANIVREVSTSSASLDVNAGIYIDPKVIINGDFNASDRASEGGRAVY
                     LYFTLIEDEVKEPYAGKKPIIFVGVPQSLPARSDLTGAIQSAFWYMDQRLSEDRVGVD
                     GLKMKVMQPALFEEKELYIRETDLGEKCTKQDIADNYPYKFFIVSDEDFDEAVMTRNE
                     DVIYLQRVLFPTTTSRIQNVFVFIDAASGKLCGMVTSYRIQERYLKNILN"
BASE COUNT         1617 a         1227 c         1491 g         1247 t
ORIGIN      
        1 catctctgac ttcagactgg aagctgaatc gccgtatccc atccccgaca gggaggagct
       61 gcgccggaaa aatatgcagc tgaagtatcc aaaagagaat gtgcatttcc gccactacgg
      121 caggtacgtg gagtccatga tcaaacaggc catcagcatg gaggatccgg aaaagcagga
      181 agcgtttaca gaggtcattg gcaattacat gaaactggtg taccagaact ggaacaggga
      241 gaatgtaaca gatgacctga tcaagtcaga tcttgaattc ctgtctggtg gtaaactgac
      301 cctgcacgaa gaaagcaacc tggatactct tagcaaggcc aaccgcacca aacaggggcc
      361 gaatcagcaa cagacccagg agagacgtca gggcggacgc taccagcgca atttcaaaca
      421 gaacaagaac cgcaatttca aacgcaaccg ctgatggggg catttgagat caaaggaggg
      481 aagtcgctca gcggagagat cattccccag ggtgccaaga acgaagcact ccaggtcatc
      541 tgtgcagtac tgctcacacc ggagtctgtt accatcacca atattccgga catcctcgat
      601 gtcaacctgc tcatagacct gctgggtgat atgggtgttg tcattgaccg gccatcatca
      661 gatacctgca ccttcactgc gagagatgtc aatctggact ttctgcaaac ggaggcattc
      721 aaggaaaaaa gccggcgtct ccgtggatct gtcatgatac ttggccccct cctcgcgagg
      781 ttcaagaaag ccagtctgcc caaacccggt ggagataaga tcggtcgccg caggctggac
      841 acccacttca taggtttcga aaaactggga gccagattca actacgatac ccacgatgca
      901 ttctatacca ttgatgcctc caggcttcat ggcacttata tgttgctgga tgaagcgtct
      961 gttaccggta cagccaacgt ggtgatggct gctgtgctgg ctgaaggcac tacgaccatc
     1021 tacaatgctg cctgtgaacc ctatctgcag cagttatgta aaatgctggt gggtatggga
     1081 gcaaagatca gcggcatcgg ctccaacatg ctcatcattg aaggtgtgga agcgttgcat
     1141 ggcacagacc atcgcatgct gcctgatatg atcgagatcg gcagcttcat cggtatggcc
     1201 gccatgacac aaagtgagat caccattaaa gatgtggctt atgaccagct cggtctgata
     1261 ccagacgtgt tcagcaggct gggcattcag atggagagga gaggagaaga cctgtacata
     1321 ccggcgcagg aacattacga gatacagacc tttattgacg gttccatcat gaccatagct
     1381 gatgcaccgt ggccgggttt tacacctgac ctgctgagta tcgtgctggt ggtggccacc
     1441 caggccaaag gatccgtact catccaccag aagatgtttg agagcaggct gttctttgtt
     1501 gacaaactca ttgacatggg tgcccagatc attctttgtg acccgcaccg tgctaccgtg
     1561 atcggactgg accgcaggaa tcagcttcgg ggtctcgaaa tgagttcacc agatatacgt
     1621 gcaggtgtgg cattgctcat cgcagcactg tcggccaagg gaaccagtat catacgcaac
     1681 atcgagcaga tagaccgggg ctatcagcgc atcgactcca ggttgcaggc tttgggtgca
     1741 tccatcgaaa ggatcgcctg acccatgcaa cgttctgtgt gatttgtcca tcaaagaatt
     1801 aagcaattac acaaaagaaa ttattgttta aaggccatgc agtaccgctc ctctctcagc
     1861 cttgcgctga ccgttttttc cctgtttttc atcctgacct cctccttcac ctgttccggt
     1921 tccaaagaag ctgtaatagg catcaagacc ggtaacctgg cgccggagat cctgtgcgat
     1981 gggatgaaag gagactgtgc cagtctgtcg tctctggagg ggaaactggt attggtggaa
     2041 ttctggacag ccgccagcaa tatttgccgg caggcgcatt ttgaaatggg ccggatgtac
     2101 aacaccttca aggatcagaa atttgagaac gccgatggct ttaccatcta ttctgtttca
     2161 cttgataatt ctgaagaaca atggctgcag gccatcaaag aggataaatg caactggccg
     2221 tatcagatgt gcgatccccg gaaatggaat gccccttctg tgctcactta tgaggtcaat
     2281 acccttccta aatatttcct gatagacggt aatggagaga tcctgtaccg gtatgtggaa
     2341 atagcagatc tggagaacct cctggaagac ctcagcgaac actgaccgtg acattttgac
     2401 aacaggaccg gcatggcagt atttttgcac atggcggtat cggtgtgtgt acaccaataa
     2461 taaccctgag tatgtcaaaa aagcaggaaa tacaagacga agagaccacc ctgtcagata
     2521 cagaaaatac tgaacaggaa cgcccggctg aaggtatgcc ggtggaggaa gagctggtgg
     2581 aagaatccag ttcgctggtg aatgaactgg agcagcaaaa ggagaaattc attcgtttgc
     2641 tggctgagtt tgagaattac aagcgacgaa ccgccagaga gcggctggat atcatcaaga
     2701 cagcgggtca ggatattatg gcagacctgc tgcctgtatt ggatgatgtg gaccgtgcat
     2761 ctgagatcat tgataaggca caggatgtgg aagccattcg tcaggggctg gtgctgatca
     2821 gtgaaaaact gaaaaacatg gtcgctcaaa aaggccttca acccatggag gtcaagggac
     2881 aggattttga cgccgagacc atggaagctg tcacggagat accggccggt gatgacatga
     2941 aggggaaggt tgtggatgtg atcgaaaaag gttatacgct taatgaaaag atcatccgct
     3001 atgcgaaagt ggtggtaggt aaataaacga gatggcgaag cgagattatt acgaagtgct
     3061 gggtgtcagt aagaatgcat ctgcggaaga gatcaagaaa gcttaccgca agatggcatt
     3121 gcagtaccat cctgacaaga accccggtaa taaagaagcc gaagaaaagt tcaaggaggc
     3181 ggccgaagcc tatgacgtac tgagcaatgc ggaaaagaaa cagcggtacg atcagttcgg
     3241 ccatgcaggc atgggtggtg ccggtggttt tggcggtgcg ggcggaggta tgaatatgga
     3301 tgatatcttc agccagttcg gcgacatttt tggtgattcc attttcgatt ctttttttgg
     3361 cggcggtgga agaggtcgtg gtgcacagcg gggaagaggt aagcggggca gcaatatccg
     3421 catcaaggtc aagatgaccc tggacgaaat tgccgatggc accaagaaga ccatcaaggt
     3481 caagaaatat atcccatgcg attcctgtag cggaacagga gccaaggatg gctctgcgta
     3541 tgagacctgt aagacctgcg gcggacaggg tgcagtgcgc agagtggcca acaccatcct
     3601 gggtcagatg cagaccacac agacctgccc gacctgtaac ggatcaggac agaccatcat
     3661 tgccaactgc ggaaaatgcc atggcgaagg aagggtgtac ggagaagata ccatcacact
     3721 cgatattcct gccggtgtga ccgatggcgt ggagctgtcc atgtccggcc gtggtaatgc
     3781 aggtgaacaa ggcggaccca acggcgatct gctcatctct gtagaagaga taccacacag
     3841 cgacctgaag cgggaaggac aacacgtact gtacaatctg tatctcaata ttgcagatat
     3901 ggccctcggt acacaggtgg aagtgcccac cattggtgga agagcccgca tcaaaattcc
     3961 ggaaggcaca caaagcggcc acatcatgcg gctcaaaggc aaagggctgc catcactcaa
     4021 ttcttacggc aaaggtgatc agatcatcca ggtgaatgtc tgggtgccga aacacatgac
     4081 cacagaagag aagaagacga tggaaaaact ccgcgatgcc aagaacttcc aaccagacga
     4141 aggcgagacc aggaaagaga agagcttcgt ggacaagatc cggggaatgt tcaattgaaa
     4201 gtatcaggta tcaggtagca agtatcaggt accgggtagc gggtacctca tgatgctatc
     4261 aggatgaaag gcggaattcg gaaggcggaa tttggaaatg aggaaatgtg aaaatgggct
     4321 tgttaacaca cccatgagaa gcctcaccat tcacccctca cttattctgg gcttgattag
     4381 ttaattccgc ccgatggctg gaagtgagga gtgcgagcag ggttatgcca atgcaggaaa
     4441 tgatgtgagc ttcttgattc ttttataaaa tgtctgtcct cttccgcaga gagtcaaaca
     4501 gctctgcctt cgaagcatat tcaaccaaca atcaatatat ttaaagcttc ggtcagagac
     4561 cggtgtgata tgaaattgaa tattcctgaa aacaacacat ccatgaaaac aaaactgatc
     4621 ctgacaaccg tcttactgtt tgccggtctt atttcctttg cccagatggg atttggaaag
     4681 gaaaaagaaa ttgttgaggt atatgagcga aaagtgatca tagtcaatga acaatatgat
     4741 gagcggacat taaaagtact cgagaaaaaa ggcaagaaag gagatatcac cacttacaag
     4801 aacgtcatca caaagtacaa cgccgatctg ctggaggctg taaaaacagc atggccgttc
     4861 gatacggtca ttgttgtgaa gaagatcgag gatgtactgg agatgaaaaa agataaaaag
     4921 tacaattatg tagtggtcag aacagccaat attgtgcgtg aggtttctac ttcatctgca
     4981 agtttagatg taaatgccgg aatctatatc gatccgaagg ttattatcaa tggagatttt
     5041 aatgcttccg accgggccag tgaaggaggg agagcagtat atctttattt tacattgatc
     5101 gaagatgagg taaaagagcc ctatgcaggc aagaaaccga tcatatttgt gggagtaccc
     5161 caaagtttgc cagccaggtc agacctgaca ggtgcaattc agtcagcttt ctggtatatg
     5221 gatcagcggt taagtgaaga cagagtgggt gtagatggat taaaaatgaa agtgatgcaa
     5281 cctgcgcttt ttgaagagaa agaactttat atcagagaaa ctgatttggg agaaaagtgt
     5341 acaaaacagg atattgctga taattacccg tataagttct tcattgttag tgatgaggac
     5401 tttgacgaag cggtcatgac caggaacgag gatgtgatct acctgcaacg ggttcttttt
     5461 cctactacta cttcccggat tcaaaatgtc tttgttttta ttgacgcggc aagcggaaag
     5521 ctctgtggta tggtaacttc atatcgaata caagaaaggt atcttaaaaa cattcttaat
     5581 ga
//
LOCUS       DAOMAX010000174         5571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2394_length_5571_cov_4.527194, whole genome shotgun sequence.
ACCESSION   DAOMAX010000174 DAOMAX010000000
VERSION     DAOMAX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_5571_cov_4.527194"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..542)
                     /locus_tag="PK742_09135"
     CDS             complement(66..542)
                     /locus_tag="PK742_09135"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HQU76791.1"
                     /translation="MKQVAILLLLVCTVGSLHAADETSRPEEGTRLSLWIPGFLVKTA
                     AGIAADKTGDPDLSILKNIGSVSLVVREGSAYSGTYDKKIERKLRRMDRREYEELVAV
                     HDEGAEVHLHIRENRKGTVKRMIVLVDDHESTFVFVSMHCSFKPEDLQSVASMALK"
     gene            complement(559..1077)
                     /locus_tag="PK742_09140"
     CDS             complement(559..1077)
                     /locus_tag="PK742_09140"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HQU76792.1"
                     /translation="MKKLMIAAAMFGISGLVSAQTVADVFFSRYEGKDGFTSVSVSEK
                     MFDMLATAAPADEIGLKEMAAGLKGIKVLSYENPEGSLRSRELYKEAEDAISLQGFSE
                     LMQVFDEGEKVRILTKDGGDSIIEELLILVSSDEEFVLVDLFGNIDLNKVSEWTRDMD
                     IKGLDQLNRTTD"
     gene            complement(1151..1621)
                     /locus_tag="PK742_09145"
     CDS             complement(1151..1621)
                     /locus_tag="PK742_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76793.1"
                     /translation="MADLVGQGIGSLRDLLKFLTDRSRSADVLKNGLLRELRDNLQLL
                     QHRNAAGANRQAIIQSLKTDAIDAAFKGNFKFHTLSSGKKVGAQHIVADRQNRYTGWD
                     EKRLITSMEGKIHELRQLGNIFPDQESAGINIPARLDNLYFQILLLSTFIYSGK"
     gene            complement(1628..2095)
                     /locus_tag="PK742_09150"
     CDS             complement(1628..2095)
                     /locus_tag="PK742_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HQU76794.1"
                     /translation="MSKVIDETEVQGQVRMVTPSLPGYLFVARTKDKGRGVFTSRAIS
                     KGEVIEVCPMIVFSRKDRLYIDSTFLYEYYFEWGKNQKKGALALGFGSVYNHSYQPNA
                     RYEPDFDLNIMEFIAIRDIVAGEEITTNYNYDPEDDSPVWWEKSQGKKKKQHP"
     gene            complement(2098..2334)
                     /locus_tag="PK742_09155"
     CDS             complement(2098..2334)
                     /locus_tag="PK742_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76795.1"
                     /translation="MDIRTLKKRVFYVIAEAIDHVNLDPSEKDAESKTDSLLTLFEET
                     VTRINAGKRAGTNARAHFRQVKNEFNSRIQEILG"
     gene            2389..2691
                     /locus_tag="PK742_09160"
     CDS             2389..2691
                     /locus_tag="PK742_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQU76796.1"
                     /translation="MFDLGKAMEMKKQMEAISKRLDTITVEGLAGSGERQVSATFTAN
                     RAIRNIRISPALLQPESKEELEDLLVLACEKAIQQAQQVAETEARNLALGGGFPGI"
     gene            2691..2891
                     /locus_tag="PK742_09165"
     CDS             2691..2891
                     /locus_tag="PK742_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76797.1"
                     /translation="MQIAEKISRWFRIIMAVLLLLICGAGCILSFREGDEQTGWILLI
                     LLVLALIYAWYAFKGKKGFGQV"
     gene            2950..4188
                     /locus_tag="PK742_09170"
     CDS             2950..4188
                     /locus_tag="PK742_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQU76798.1"
                     /translation="MPARLILILCFITAIDLYALQAILTIAGGGWTVLLPWFSLTVLS
                     LLSMYLFFAFSARERLGWSRQLVAAASFITLMPKVFIALLLIPEDIYRLGLSLILVIG
                     GLFTEMPDWHTGRSEVWSWVAITASLSMMTLFLYGALFNVYKYRVRHIGLKAIRLPQV
                     FSGLKVIQISDIHSGSLSSKDGVQKAVDRINAQQPDLVFFTGDLVNNIADEALFLQDV
                     FSGIQARLGVFSVLGNHDYGDYVAWPSEREKAANLSKLKRIHADMGWRLLMNEHVMIQ
                     HEGQQIAVAGVENWSASDRFPKYGKLDTALHGIDPNTPVLLLSHDPSHWEAEVLDHPA
                     YIMATFSGHTHGMQFGFEWGRFRWSPVQYVYKQWAGLYAKADRYLYVNRGFGVLGYPG
                     RVGILPEITVFTLQHVPASE"
     gene            4232..4960
                     /locus_tag="PK742_09175"
     CDS             4232..4960
                     /locus_tag="PK742_09175"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQU76799.1"
                     /translation="MAFFLFLLLHWYLSLFSQTFFLHRYAAHSMFQMHRGWERFFYIF
                     TWITQGSSYLSPYAYGVLHRLHHAHTDTEDDPHSPSFEPGLFPLMFKTWKIYNAINTD
                     QYPAEEKYLKNLPAWRSFDRFAGSDLMRIIWVLIYAGIYALLATSWWMWLFFPITCLM
                     GPVHGAIVNYFAHKYGYRNFRMEDTSRNFLPVDILMMGESYHNNHHKYISRPNFGYRW
                     FEIDPTYPVILLLDKIGIIRLQTS"
     gene            5054..>5571
                     /locus_tag="PK742_09180"
     CDS             5054..>5571
                     /locus_tag="PK742_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76800.1"
                     /translation="MLLFAATAGFGQTGNPQQHTYFPNSDSLKIDSLSLVPETVEVLC
                     FFHGQVAAACTDSIDHWNATLYFSGDIPDSVQVKYQTFPYAFSSRWYHKDPDELRQAD
                     LDLTPYEIKANDRYDPFADLDGLQYSGSLVRGLSFGNNQDVVLNSSFNLQMSGMLQND
                     VEITAAITDNNIP"
BASE COUNT         1391 a         1353 c         1256 g         1571 t
ORIGIN      
        1 tcgcctttcg tttctgacat aaaaaagggg gctggatgca gccccctttt ttatattgat
       61 ccgggtcact ttagtgccat cgatgctaca gactgcaggt cttccggttt gaaactgcag
      121 tgcatgctga caaagacgaa tgtactttca tgatcatcca ccagaacgat cattcgcttc
      181 actgtgccct tacggttttc tctgatgtgc agatgcactt cagcaccttc atcatgcacc
      241 gctaccaact cttcgtactc tctgcgatcc atgcgccgga gtttacgttc gatcttttta
      301 tcataggtgc cggagtacgc tgatccttct ctgactacca gcgaaacact accgatattt
      361 ttcaatattg acaggtccgg atcgccggtc ttgtctgctg ctataccggc ggctgtcttt
      421 accagaaacc cgggaatcca aaggcttagt ctggtacctt cttcaggccg ggaggtttca
      481 tctgctgcat gcaaagatcc aacagtacag accaacagca aaagaattgc aacttgcttc
      541 atgatgctac tttttatttc agtcagtggt tctattcagt tggtccaggc ctttgatgtc
      601 catgtctctg gtccattcag agaccttgtt cagatcaata tttccaaaca gatcaaccag
      661 tacaaattcc tcatcgctgc ttaccagaat gagcaattct tcgataatgg aatcgccgcc
      721 atctttagta agtatcctga ccttttctcc ttcatcgaat acctgcatga gttcagagaa
      781 accctggaga gaaatggcat cttctgcttc cttgtacagt tcgcggctgc gcagtgaacc
      841 ttccgggttc tcataagaca aaaccttaat accttttagg cctgcagcca tttctttcag
      901 cccgatctca tctgcaggag ctgcagtggc aagcatatca aacatttttt cggaaacgct
      961 tacgctggta aagccatcct tgccttcgta ccgggagaaa aatacatctg ctacggtttg
     1021 tgcagaaacc agtccggata taccaaacat ggctgcagcg atcatcagtt ttttcatgtt
     1081 cctgttgttt tatggttatc aaacaaccat aggtcgtaca gtcacatgaa atagttacag
     1141 catgcctgca ttattttcct gaatagatga aagttgacag caaaaggatc tgaaaataca
     1201 ggttatccag ccttgcaggt atattgatgc ctgcactttc ctgatcagga aaaatattcc
     1261 ccagttgtct gagttcatgg atctttccct ccatgcttgt gatcagcctt ttttcatccc
     1321 agcctgtata tcggttctgt ctgtcggcca ctatatgttg tgctccaact tttttccccg
     1381 atgacaaagt gtgaaattta aaattcccct tgaaagctgc gtcaatggca tctgttttca
     1441 gcgactgaat gatcgcttgt ctgtttgctc cggcggcatt cctgtgttgc agtaactgaa
     1501 gattgtctcg gagttcacgc aatagaccat tcttcagaac atcagcagat ctggatcggt
     1561 ctgttaaaaa tttcagcaga tcacgaaggc tgccaatacc ttgtccaacc aggtcagcca
     1621 tagtgcatta aggatgctgt ttcttcttct tcccctggct tttttcccac caaacaggcg
     1681 aatcgtcttc cgggtcgtaa ttatagttgg tcgtgatctc ctcaccagcc acgatatcgc
     1741 gaatggcaat aaactccatg atattcaggt caaaatcggg ctcataacgt gcatttggct
     1801 gatagctgtg gttatagaca gatccaaagc cgagggccag ggcacctttc ttctgattct
     1861 ttccccattc aaagtaatac tcatacagaa aagtactatc tatatacaga cgatcctttc
     1921 ttgaaaacac gatcatcggg caaacctcaa taacttctcc tttgctgatg gctctgctgg
     1981 tgaagacacc cctgcccttg tctttggtac gggcaacaaa aagatagccc ggcaatgacg
     2041 gggtgaccat tctcacctgg ccctgtactt ccgtttcgtc aatgaccttt gacatgatca
     2101 gcccaagatc tcctgtatcc tgctattgaa ctcattcttt acctgacgga aatgagccct
     2161 tgcgttggta ccggcccttt taccggcatt gatccgggta actgtttctt caaacagggt
     2221 cagcaaagag tctgtcttgg attctgcatc cttctctgaa ggatcaagat tgacgtggtc
     2281 tatcgcttct gcgatgacat aaaagacccg ttttttaagt gttctgatgt ccatagctaa
     2341 tctgtgtcaa agtaagcaaa taaatacctt tgtggcaaaa tatcgcatat gttcgatctg
     2401 ggtaaagcaa tggagatgaa gaagcagatg gaagccatct caaagcgact tgacaccatc
     2461 acggtagaag ggctggcggg tagcggagag cgacaggtat cagccacctt tacagccaac
     2521 cgggctatca ggaacatccg tatcagcccg gcattgttgc aacctgaaag taaagaggag
     2581 ctggaagacc tcctggtact ggcctgtgaa aaggccattc agcaggctca gcaggtagct
     2641 gaaacagaag ccagaaacct ggcattggga ggaggatttc ccggaatctg atgcagattg
     2701 ctgaaaaaat atctcgttgg ttcaggatca tcatggctgt gctgttgctg ctaatttgcg
     2761 gggcaggatg cattttgtct tttcgtgagg gcgatgaaca gaccggctgg attctgttga
     2821 tcctgctggt attggcacta atctatgcct ggtatgcttt caaggggaag aaaggatttg
     2881 gccaggtttg acaatatcag tccggtgtaa tcgtcggcaa caatactgac acaacattgt
     2941 tagacgaaga tgcccgcaag actgatcctg attttgtgtt tcataaccgc cattgacctg
     3001 tatgccctgc aggccattct gaccattgcc ggtggcggat ggactgttct gttgccttgg
     3061 tttagcctga ccgttttatc tctgttaagc atgtacctgt tctttgcttt ttctgccaga
     3121 gaaagactgg gttggagccg gcaactggtt gctgctgcat ctttcatcac cttaatgccc
     3181 aaagtattca ttgcacttct actgataccg gaagacattt acagactggg gcttagcctg
     3241 attttagtaa tcggtggatt gtttacagaa atgcctgact ggcataccgg tcgctctgaa
     3301 gtttggagct gggtggcaat tacagcatcc ctgtctatga tgacattatt cctgtatgga
     3361 gcactattca atgtttataa ataccgggtc aggcatattg ggctgaaagc gatcagattg
     3421 ccacaggtat tcagcggact gaaagtgatc cagataagtg atattcacag cggcagcctg
     3481 tccagcaaag acggtgtaca aaaagccgtt gaccgcatca atgcgcaaca accggatctg
     3541 gtatttttta ccggcgatct ggtcaataat atcgcagatg aagcactttt cctgcaggat
     3601 gtgttcagtg gtattcaagc cagacttggt gtattcagcg tactcgggaa ccacgattac
     3661 ggcgactatg tagcctggcc atctgagcgg gaaaaagctg caaatctcag caagctgaaa
     3721 cgcattcatg ccgatatggg atggcgtttg ctcatgaatg agcatgttat gatccaacac
     3781 gaaggacagc agatcgctgt ggcaggtgtc gagaactgga gtgccagcga tcgttttcca
     3841 aagtatggaa agctggatac cgcactacac ggaattgacc caaatacacc tgtcctgctg
     3901 ttgtctcacg acccatctca ctgggaggca gaggttctgg atcaccctgc ctatatcatg
     3961 gccacattca gtggtcatac acatggtatg caattcggat ttgaatgggg gcgatttcgc
     4021 tggagtcctg ttcaatatgt ttacaagcaa tgggccggtt tatatgcgaa agcagaccgc
     4081 tacctatacg taaatcgtgg atttggtgtt cttgggtatc ccggcagagt gggtatcctg
     4141 ccagagatca ctgtctttac cctgcagcat gtgcctgcat cagagtgacc ctgttttttc
     4201 tatgcattaa ctttgcactt caattttctg catggccttt tttctgtttc ttcttctgca
     4261 ctggtaccta tccttgttca gtcagacctt ttttcttcat cggtatgcgg cacacagcat
     4321 gtttcagatg caccgcggat gggaaaggtt cttttatatc ttcacctgga taactcaggg
     4381 atcctcctat ctgtcacctt atgcatatgg ggtattgcac aggcttcacc atgcacatac
     4441 agataccgaa gatgaccccc actcccccag ttttgaaccg ggattatttc cattgatgtt
     4501 caaaacctgg aagatctata atgccatcaa tacagaccag tatcctgccg aagaaaaata
     4561 tcttaagaac ctgcctgcct ggaggagttt tgaccgcttt gccggaagtg acctgatgcg
     4621 catcatttgg gtgctgatct atgccggcat ctatgccctg ctggccacca gctggtggat
     4681 gtggctgttc ttccccatta cctgcttgat gggacctgtt cacggtgcca tcgtaaacta
     4741 ctttgcacat aaatacggct accgcaattt cagaatggaa gatacctcca gaaacttcct
     4801 gccggttgat attctgatga tgggcgaaag ctaccataac aaccatcata aatatatcag
     4861 tcgccccaac tttggctatc gttggtttga aattgacccg acctatcctg tcattctgct
     4921 cctggacaaa atcgggatca tcagactaca gacttcctga tatactttca cgcaggctaa
     4981 agcgttagct gaaagccagt aatttcgtca ggaattaaag ggagccagtc tgttaaagtt
     5041 ccggatcata ttcatgctgc tgtttgctgc aaccgccgga ttcggtcaga ccggaaaccc
     5101 acaacaacac acgtattttc ccaacagtga cagcctgaaa attgacagtt taagcctggt
     5161 tcccgaaact gtggaggtgc tctgcttctt tcatggtcag gttgctgcag catgtactga
     5221 cagcatcgat cactggaatg ctaccctgta cttttcagga gatattcctg attccgttca
     5281 ggtaaaatac caaacctttc cctacgcatt tagcagcaga tggtaccaca aagacccgga
     5341 tgagctcagg caggcagatc ttgacctgac cccatatgag ataaaagcaa atgaccgcta
     5401 tgatcccttc gctgatctgg atggccttca atacagtgga agtcttgtaa gagggctttc
     5461 ttttggtaac aaccaggacg tagtgctcaa ctccagtttt aacctgcaga tgagtgggat
     5521 gctccagaat gatgtggaga tcactgctgc catcacagac aacaatatcc c
//
LOCUS       DAOMAX010000175         5518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2429_length_5518_cov_4.298737, whole genome shotgun sequence.
ACCESSION   DAOMAX010000175 DAOMAX010000000
VERSION     DAOMAX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_5518_cov_4.298737"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PK742_09185"
     CDS             <1..819
                     /locus_tag="PK742_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76801.1"
                     /translation="SAWLVGRTRFPGEDTKLKDRQQTVFAGMMLFWPAGLMYLSVPLR
                     ETYILLATALFLAGFFSYLQKRKFLMLLTGSVLLLLLRLQLFVCLAPLAAFLIVLRSD
                     VHRIWKLTTVLITIGLVLTYLRFMILGEPFSPEALSALRNEYIADGGNLAYGNVAWQS
                     YGDMLVDMPFLVLQFMLSPMPIFSSYDTTGMTLANLDVILVITLLLLILADLRFHWKH
                     YRYLLAIVVVFAVVFAMYEYHLTGAVRHRMPMVMMLMIPAAATAGRLWGRFRHD"
     gene            812..1600
                     /locus_tag="PK742_09190"
     CDS             812..1600
                     /locus_tag="PK742_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU76802.1"
                     /translation="MIEIGISTIDDRIKQACMILDQRLANVRYRVCHQVTDSKDHSHV
                     YERYPDVEFLVMHEKGLSKSRNRLLHSSHADWLYLCDDDVIPENISHLLKTAVQDQPD
                     TSICCFMLKDENGHALKRYAAEAYQINRRTAGGVSSAEVIYHLPSLLQAGLAFDERFG
                     LGSEYISGEENLLLDQALKKGLKIRFFPMYIGSHPALRTGILFTPELVRSKGALHAAR
                     YGAGATLMNLLFACRKFRLYKDQMSPAAFLRYIYSGSKDYQRHG"
     gene            1593..2354
                     /locus_tag="PK742_09195"
     CDS             1593..2354
                     /locus_tag="PK742_09195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU76803.1"
                     /translation="MDEPLVSVIIPVYNAEHYLRSSVQSVLDQSWKQLEILLIDDCST
                     DNSREIMLELSVHPNIRSIYMPVNSGAAATRNAGILEATGRFIAFLDADDRWMPDKLE
                     RQIPFMMREKCALTCTAYDWVEANGAPAGRTIHAPQTITHRDLLKHNSIGCLTAVYDT
                     TICGKLYMPDTPQRHDWGLWLSITRQFGPAKGLDLVLAAYRLHDQSLSRNKLRSARYN
                     WIILREHEGLSRPVAAWYYLHFLIRKSWKYLGWNS"
     gene            2359..3273
                     /gene="queG"
                     /locus_tag="PK742_09200"
     CDS             2359..3273
                     /gene="queG"
                     /locus_tag="PK742_09200"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQU76804.1"
                     /translation="MTPEVHSRIIRELAAKHGFAFAGLSKAEKLEEEARRLEQWLKQG
                     SHGKMHYMENHFDKRVDPTLLVPGAKSVISLMYNYYPKEEPATDNKLKIARYAYGEDY
                     HYVIKDILRAMVADLQQQIGDFHARIFVDSAPVLEKAWARKSGIGWQGKNTNLIHPKA
                     GSFYFLAEIICDIEVMADGPIKDHCGTCTACLDACPTEALYEPYKLDASRCISYLTIE
                     LKDEIIPGEFHDKMEGWMFGCDICQEVCPWNRFSKPHRQPRFHPDESVMHMDRNDWME
                     MTDEVMLALIKNSPLSRPGPAGIRRNLL"
     gene            complement(3275..3868)
                     /locus_tag="PK742_09205"
     CDS             complement(3275..3868)
                     /locus_tag="PK742_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HQU76805.1"
                     /translation="MTKMLGLKLPTDPRWVNLAEMSLEEVLTDHAYCEQKAASTCISL
                     IQQYPEREELVEQLAPVVTEEWGHFRMVMKELKRRELKLGRQRKDEYVNRLIEFRKKG
                     QNGKEEVLLDQLLMAALIEARSCERFRLLSEGLEEEHLRKFYREFMVSEAGHYRMFLD
                     LANTYCSKERVKERWQEWLEYETLVMQELELRGERMH"
     gene            3891..4916
                     /locus_tag="PK742_09210"
     CDS             3891..4916
                     /locus_tag="PK742_09210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQU76806.1"
                     /translation="MPKILVIRFSSIGDIVLTTPVLRCIKEQVGDASIHYLTRRPYVS
                     LLQANPHIDQIHAFDDDLEDTLRRLRKEQFDFVVDLHHNLRTWRVKRSLKRPGASFHK
                     LNIAKWLMVRFKWNRLPDEHIVQRYIRAASGLGVIDDGKGLEHFIPPDTNDQRDLLPD
                     TYRNGFIALVIGAQHATKKLPLQKLQELLDKLSVYRTGVVIIGGPEDASEAEALCTNR
                     QQVFSMCGISSLQESALLIKHAVVVITHDTGMMHIAAALKKQVISVWGNTIPEFGMYP
                     YFGSQNTLYEQRAAELLIENTSLSCRPCSKIGFEACPKGHFRCMMDLNMEAIAGKAGE
                     LNATANQ"
     gene            4926..>5518
                     /locus_tag="PK742_09215"
     CDS             4926..>5518
                     /locus_tag="PK742_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HQU76807.1"
                     /translation="MLDVLMNISPIDGRYKAQVRDLEGYFSEFALFKYRLFVEVEYFI
                     SLTEIPLPQLQQFDHNKRSDLRNIYKKFTPESCLRIKEIEKTINHDVKAVEYYIKEHF
                     DQLGLEAYKEFIHFGLTSQDINNTAVPISFRDALLDVYAPVLEDIVGQLSELAGQWST
                     IPMLARTHGQPASPTMLGKEIRVFIERLNGQRSLLNN"
BASE COUNT         1407 a         1382 c         1322 g         1407 t
ORIGIN      
        1 tctgcttggc ttgtcgggcg gacccgtttt ccgggcgagg atacaaaact caaagacaga
       61 caacaaaccg tatttgcagg tatgatgctg ttctggccag ccggactgat gtacctgtct
      121 gtgccactca gagagaccta tatcctgctg gccactgcac ttttcctggc aggattcttc
      181 tcttatctcc agaagagaaa attcctcatg ctgctcacag gcagtgtgct cctcctgtta
      241 cttcgtttgc agttgttcgt ttgccttgct ccgttagctg cattcctgat cgtgctgcgt
      301 tctgatgtgc accggatatg gaaactgaca accgtattga tcaccatcgg cctggtactc
      361 acctacctca gattcatgat actgggtgaa cctttcagcc cggaagcgtt gtcagcgttg
      421 cgaaatgagt acatcgccga tggtggtaac ctggcatacg gtaatgtggc ctggcagagt
      481 tatggtgaca tgctggttga tatgcctttt ctggtattgc agttcatgct gtcacctatg
      541 cccatattct ccagctacga tactacagga atgacccttg ccaatctgga tgtcattctc
      601 gttatcacac tgctgttgct gattctggct gatctgcgtt ttcactggaa acactatcgt
      661 tatcttctgg caattgtagt ggttttcgct gtggtatttg ccatgtatga atatcatctg
      721 accggtgccg tgcgacatcg catgccgatg gtcatgatgc tcatgatacc ggctgccgct
      781 accgctggca ggttgtgggg aagattcaga catgattgag atcggcatat caaccattga
      841 tgacaggata aaacaggctt gtatgatcct ggatcagcgc ctggcgaatg ttcgctacag
      901 ggtttgtcat caggtgaccg acagcaaaga ccattcacat gtgtatgagc gctaccccga
      961 tgtggaattc ctggtgatgc atgaaaaagg cctgtccaaa agccggaaca gattactgca
     1021 ttcttcccat gcagattggc tctatctgtg cgacgatgat gtaatacctg aaaatatttc
     1081 tcatttgtta aagacagctg ttcaagatca gcctgatact tccatctgct gtttcatgct
     1141 gaaagatgaa aacggccatg ctctgaagcg gtacgctgct gaagcatacc agatcaaccg
     1201 cagaactgca ggaggtgtga gcagtgcgga agtgatctat cacctgccct ccttgctaca
     1261 ggcaggtctt gcatttgatg agcgtttcgg actgggatca gaatatataa gcggagaaga
     1321 gaatctgctg cttgatcagg ctttgaagaa aggactgaag atccggttct tcccgatgta
     1381 tatcggaagc catccggcat taagaacagg tatcttgttc acaccagagc tggtgcgttc
     1441 aaaaggtgcc ttacatgctg cccgctatgg agcaggtgca acgctgatga atctgttgtt
     1501 cgcctgtcgc aaattcagac tctacaaaga ccagatgtcg ccggcagcat ttcttaggta
     1561 tatttattcc ggaagcaagg actatcaacg gcatggatga acctctcgtt tctgtcatta
     1621 ttccagtgta caacgctgaa cactacctgc gcagttccgt gcagtctgtg cttgatcagt
     1681 cctggaaaca gctggagatc ttgctgatag atgattgttc cactgataac agccgtgaga
     1741 tcatgctgga gctcagtgta catccgaaca ttcgttccat ttatatgccc gtgaacagcg
     1801 gagctgctgc tacccgcaat gcgggcattc tggaggcgac aggcagattc attgcctttt
     1861 tggatgccga tgacagatgg atgcctgata aactggaaag acagataccc ttcatgatga
     1921 gggaaaaatg cgcattaacc tgtactgcat acgattgggt ggaagcaaac ggagcccccg
     1981 caggacgaac cattcatgca ccacaaacca tcacccaccg agatctgctc aaacataaca
     2041 gcattggctg cctcacagca gtatatgata cgacgatctg cggtaagctg tatatgcctg
     2101 atacaccaca gcgccatgac tggggtttat ggctttccat cacccggcag tttggaccgg
     2161 caaaaggcct cgatctggtg ctggctgcct accgtttgca tgatcagtcc ctatccagga
     2221 ataaacttcg gtcggccaga tacaactgga tcattctgag agaacatgaa ggtctctccc
     2281 gacctgttgc agcatggtat tacctgcatt ttctgatcag aaaatcctgg aagtatctgg
     2341 gctggaattc ctgaaattat gacccctgaa gtacacagca ggatcatcag agaactggcc
     2401 gcaaagcacg gttttgcatt tgccggactg tctaaggctg aaaagctgga ggaagaagcc
     2461 cgccggctgg aacagtggct gaagcagggt tcgcacggaa agatgcacta catggagaat
     2521 cattttgaca agcgggtcga tcctactttg ctggttccag gtgcgaagag cgtgatcagt
     2581 ctgatgtata actattatcc gaaagaagaa cctgcaactg acaacaaact gaagatcgcc
     2641 aggtatgcgt atggcgaaga ttaccattac gtgatcaaag acatccttcg ggcaatggtg
     2701 gctgacctgc agcaacagat cggcgatttc catgccagga tcttcgtaga ttctgctcct
     2761 gttctggaga aagcctgggc cagaaaaagc ggcatcggat ggcagggaaa gaacaccaac
     2821 ctcatccatc ccaaggccgg ttccttttat tttctggccg agatcatctg cgatatcgaa
     2881 gtgatggcag acggccccat caaagaccat tgcggcacct gcaccgcatg tctggatgcc
     2941 tgccctactg aagccttata tgaaccttat aaattagatg cctcccgctg catcagctac
     3001 ctgaccatcg aactaaaaga tgagatcatt cccggcgaat ttcatgacaa gatggaaggc
     3061 tggatgtttg gctgtgatat ctgccaggaa gtatgtccgt ggaatcgttt ttccaaaccc
     3121 caccggcaac ccagattcca tccggatgag tccgttatgc atatggatcg caatgactgg
     3181 atggaaatga ccgatgaagt gatgcttgcc ctgatcaaaa attcaccgtt aagccgaccc
     3241 ggacccgccg gcatcagaag aaatctgttg tgaatcagtg catgcgctca ccgcgaagct
     3301 cgagctcctg catgaccaat gtttcatatt caagccattc ctgccaccgt tccttaaccc
     3361 gctctttgct gcaataggta ttggccagat ccaggaacat gcgatagtgt ccggcctctg
     3421 agaccatgaa ttctctgtag aatttccgga gatgttcttc ttccagccct tcactcagta
     3481 accggaaacg ctcacaactg cgggcttcta tcaaagctgc catcagcaac tgatccagta
     3541 acacctcctc ttttccgttc tggcctttct ttctgaattc gatcagcctg ttcacatact
     3601 cgtctttgcg ctgccggccc aacttcaatt ctcttctttt cagctctttc atgaccatgc
     3661 gaaaatgccc ccactcttct gtgacaacgg gtgccagctg ttctaccagt tcttcccgct
     3721 caggatactg ctgaatcaga ctgatgcagg tagaagctgc cttctgttca cagtaggcat
     3781 gatctgtcag tacttcttcc aggctcattt cagcaaggtt tacccatcgc ggatctgttg
     3841 gtagtttcag tcctaacatt tttgtcattt ttgtacacca aaggtagcca atgcccaaga
     3901 tccttgtcat ccgcttttcc agtatcggcg atatcgtgct gaccactccg gtgctgcgat
     3961 gcatcaaaga gcaggtgggt gatgcatcta ttcactacct gaccagaaga ccttatgtaa
     4021 gcttgttgca ggcgaacccg catatcgatc agatccatgc ttttgatgat gacctggaag
     4081 atacattgag aagattgcgc aaagagcaat ttgactttgt ggtggacctg catcacaacc
     4141 ttcgcacctg gcgtgtgaaa cgatccttga aaagaccggg tgcttctttc cataaactga
     4201 acatagccaa atggctgatg gtgcgtttca aatggaacag actgcccgat gaacatattg
     4261 tgcagcgcta tatacgtgca gcttccggtc tgggagtgat cgacgacggg aaaggcctgg
     4321 aacatttcat tcctcccgat accaatgacc aaagagatct gttgccagac acatatcgga
     4381 atggtttcat agccctggtc atcggcgccc agcatgccac caaaaaacta ccgctgcaaa
     4441 aactgcagga acttcttgat aagctatcgg tatatcgaac aggagtggtg atcatcggcg
     4501 gacctgaaga tgcttccgaa gcagaagcat tatgtaccaa caggcaacaa gttttcagta
     4561 tgtgcggaat tagcagcctg caggaatctg ccttactgat caaacacgca gttgtggtca
     4621 tcacccatga taccggtatg atgcacattg ccgcggctct gaaaaaacag gtgatctctg
     4681 tctgggggaa taccatccct gagtttggca tgtatcccta ctttggcagc caaaacaccc
     4741 tgtacgaaca aagagcagca gaactgctca ttgaaaacac ctccctttcc tgtcgcccct
     4801 gctccaagat cgggtttgaa gcatgcccaa aaggacattt cagatgtatg atggacctga
     4861 acatggaggc tatcgccggg aaggccggag aactcaatgc gaccgcaaat cagtaatttt
     4921 gcagcatgct ggatgtgctg atgaatattt ctcccattga cgggagatac aaagcccagg
     4981 ttcgcgatct ggaaggctat ttttctgaat tcgccctgtt caaataccgt ctgttcgtag
     5041 aggtagaata cttcatctcc ctgacagaga tacccttgcc tcaactgcag cagtttgacc
     5101 acaacaaaag aagcgacctg cgaaatatat ataagaagtt tacacctgaa tcatgcctcc
     5161 gcatcaaaga gatcgaaaag accatcaacc atgatgtcaa agcagtagaa tattatatca
     5221 aagaacattt tgatcaattg ggtctggagg cttacaagga gtttatccac ttcggactga
     5281 ccagtcagga catcaacaat accgctgttc ctatcagctt ccgcgatgcg ctgctggatg
     5341 tatatgcgcc tgtgctggaa gatatagtcg ggcagctcag tgaactggcc gggcaatgga
     5401 gtaccattcc aatgctggca cgtacccatg gacaacctgc ttctcccacc atgctgggaa
     5461 aggagatcag ggttttcatt gaaaggctga acggacagcg cagtttgctc aacaatat
//
LOCUS       DAOMAX010000176         5500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2446_length_5500_cov_4.166575, whole genome shotgun sequence.
ACCESSION   DAOMAX010000176 DAOMAX010000000
VERSION     DAOMAX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_5500_cov_4.166575"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PK742_09220"
     CDS             complement(<1..222)
                     /locus_tag="PK742_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76808.1"
                     /translation="MISDKVSASAQIGIGIMLIILGVLVTPIGLIGAIAGGFTYVGIL
                     MMFLLLGGALMLLGGIQYLRAGLKLSRDEK"
     gene            262..1632
                     /gene="mnmE"
                     /locus_tag="PK742_09225"
     CDS             262..1632
                     /gene="mnmE"
                     /locus_tag="PK742_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQU76809.1"
                     /translation="MAYLENDTIVALSTPAGMGAIAVIRLSGSDAIRMCDAVFQGTDL
                     LEAAANTLHVGWLKDGDQLVDEVVVGIFRAPKSYTTEDVVEISCHGSVFIQQQVLALM
                     IKQGARMAKAGEFTMRAFLHGRMDLSQAEAVADLIASEGAAAHDLAIKQMRGGFSNEL
                     SKLRDQLIGFASMIELELDFSEEDVEFANRDELVVLSNRIEKVLDQLIDSFRLGNVIK
                     QGVQTVIAGRPNAGKSTLLNALLNEDRAIVSEIAGTTRDTIEEEVNIHGILFRLIDTA
                     GIREATDVIEQAGVQRTMEKIAQSTLLLYVFDVQEMSVEEVGKDLQQFHQGNYKLLLV
                     GNKMDRGLPGDKVLALDAFGDVLYISSKDGSNLDALKERMYQLVGGDNRLGDVVVTNV
                     RHLEALTKAKGAIQEVLDGIKGKFSGEITALSIRRALNYLGEITGEVTHEDLLDYIFS
                     KFCIGK"
     gene            1856..2206
                     /locus_tag="PK742_09230"
     CDS             1856..2206
                     /locus_tag="PK742_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQU76810.1"
                     /translation="MKEFSNIELKRMTDDMIIKAIGEYIRSVRLDRNLTQEQLGERSG
                     VHRTTIRDLELGKRSTLLTLIQVLRSLDQLQTLKNFKVSKELNPLELAKLEMEERKRA
                     SGSRSNQKPKTSDW"
     gene            2206..3507
                     /locus_tag="PK742_09235"
     CDS             2206..3507
                     /locus_tag="PK742_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HQU76811.1"
                     /translation="MIKAAYVNIWNKRVGAVAWNEEEGIATFRYDPEFLETGLDLSPV
                     MMPLNESLNYRFPEHRNTTTFKGLPGMLADVLPDKYGNALINQWLVKHNRPANSINPV
                     ETLCFIGKRAMGALEFEPSEPKITGHSIKLEMNELIDITSKILSGRQAFLTNLQPDQE
                     KALQAILKIGTSAGGARAKALIEFNKETKEIRSGQANAPKGFDHYLIKFDGIEDEQFG
                     TSRGYGRVEMAYYKMAVDAGIEMMESELLEENGRAHFMTKRFDRVQGKGKIHVQSFCA
                     MRHYDFKDIDIYSYEDLFETMRLLVLPYPQAEQLFRRMVFNVIARNCDDHTKNFAFTM
                     DKSGEWSLSPAFDVCHAYRPSSTWVSQHCLSVNGKRKNIERADLLEVARKMNIKGASE
                     IIDQVDQVIGKWKKYADDQKVDPELREAIKSTFVRMNKART"
     gene            complement(3929..4546)
                     /locus_tag="PK742_09240"
     CDS             complement(3929..4546)
                     /locus_tag="PK742_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQU76812.1"
                     /translation="MRTCSHCGKENPDDASYCNHCGNSMAFSKPPATSRWKRIPSWGW
                     ILILVVGVIGLIAFFIGSFVAIATIEGVASAVMLIAGMIGFGVTPLRKPQNTSGFTRA
                     LGIAFFALMGISVDQTGNYLYNKPVEMTMCDGGTLTRKENVSNPVPGSTYIEQDFVCY
                     DDNGEPIKRLNIFAVMAIRFLEYILLGYLLLFARRVLWNATRGSS"
     gene            complement(4543..>5500)
                     /locus_tag="PK742_09245"
     CDS             complement(4543..>5500)
                     /locus_tag="PK742_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HQU76813.1"
                     /translation="IGKGLKIPEMWDEALNGNLKALWLMGEDVVQTDPNTQRVMKALD
                     NLDLLVIQEIFHTETTKHADVIFPASSFLEKSGTFTNGERRIQRVNAVVEPLPGTKPD
                     GQIICEVMTHMGFPQPDYHPRWVLEEVSQIVPFFAGVKWDELGEQGKQWPVKPDGTGT
                     EILHTEDFKRGKGKFHFEPWRESEEINANGKDYPYIITTNRELEHYNAGTMTRRTRNV
                     QILTEDTLMIHPDDAAANGIADGDMVCVESPRGKVDIKARITDEVRPGVLSSTFHFPD
                     IMLNNITSDVHDSEAMCPEYKVVACRIRKSKGKFKHQPATEL"
BASE COUNT         1513 a         1213 c         1385 g         1389 t
ORIGIN      
        1 tttttcatcc cggctgagtt tcagtccggc acggaggtat tgaatgccac ccagcaacat
       61 cagggcgccg cccagcagca ggaacatcat cagtatgccc acataagtga agcccccggc
      121 gatggcaccg atgagtccaa taggggtaac gagtactccc agaatgatca gcatgatgcc
      181 gatgccgatc tgtgcggaag cgctgacctt gtcagagatc atagccgcaa attaaggcaa
      241 tttgatggtt acctttgatc catggcctat ctggagaacg ataccattgt ggcattgagc
      301 acacctgccg gcatgggagc cattgcggtg ataagactgt ctggttccga tgccattcgc
      361 atgtgcgatg cggtttttca gggcacagac ctcctggaag cagctgccaa taccctgcat
      421 gtgggctggc tgaaagatgg tgatcagctg gtagatgagg ttgtggtagg catttttcgt
      481 gcaccgaaga gttataccac agaagatgtg gtcgaaattt cctgtcatgg ttccgtattc
      541 atccagcagc aggtacttgc actcatgatc aaacagggag ccaggatggc caaagccggt
      601 gagttcacca tgcgggcctt tttacatgga cgtatggacc tgagtcaggc ggaggcagta
      661 gctgatctga tcgccagcga aggagccgca gcacatgacc ttgccatcaa acagatgcgg
      721 ggagggtttt ctaacgagct gtcaaaattg cgggaccagc tcattggatt cgcttccatg
      781 atagaactcg aactggactt cagtgaagaa gatgtggagt tcgccaaccg cgatgaactg
      841 gtggtattga gtaaccggat cgaaaaggta ctcgatcagc tgatcgatag tttccgcctg
      901 ggcaatgtca tcaaacaggg tgtgcagacg gtcattgccg gcagacccaa tgcgggtaag
      961 tccacgttgc tgaatgccct gctcaatgaa gacagggcca tcgtcagtga gatagcaggc
     1021 accacgagag atacgatcga agaagaggta aatatccatg gtatcctgtt ccggctcata
     1081 gatacggcag gcatccgcga agcgactgat gtgatcgagc aggcaggtgt gcagcgcacc
     1141 atggagaaga tcgcccagag tacattgttg ttatacgtgt tcgatgtgca ggaaatgtca
     1201 gtcgaagaag taggtaagga cctgcaacaa ttccatcagg gcaactataa gttactgctg
     1261 gtgggaaaca agatggaccg cggacttccc ggcgataagg tcctggcgct ggatgccttc
     1321 ggcgatgtct tgtatatctc ttcgaaagac ggatccaatc tggatgccct gaaggaacgc
     1381 atgtaccagc tggtaggtgg cgataacagg ttaggcgatg tggtagtgac caatgtgcga
     1441 cacctcgaag ccctgaccaa agcgaaagga gctatccagg aagtgctgga cggtatcaag
     1501 ggaaagttca gcggcgagat cacggcctta tctatacgaa gagccctcaa ctacctgggt
     1561 gagatcaccg gcgaggtgac ccacgaagac ctgctggatt atatcttcag caagttttgt
     1621 atcggaaagt aaccgacaca agagtgaata tatctgcata tagaagcatc tcaggacggc
     1681 agaccggttg aagttaaagt ttacatgatt gaagtaatct gatgctttaa tgcatcaata
     1741 actgattata ttagctataa tgatgtttaa atgcatcatt gatttcctcg ttaagtcttt
     1801 cattttactt atttttgatg ttaaattgca tcataaaagt aaagttagtg ccattatgaa
     1861 ggaattttcc aacatagaat taaagcgaat gaccgatgac atgatcatta aggctattgg
     1921 agaatacata cgatctgtac gactagacag aaatttgacc caggaacaat taggtgaacg
     1981 gtcaggtgtt catagaacaa ccatccgtga ccttgaatta ggaaaaagaa gcacactgct
     2041 gacactcata caagtattgc gatcattgga ccagttacaa actttgaaaa acttcaaggt
     2101 ttcaaaggag ttgaatcccc ttgaattggc aaagcttgaa atggaggaaa gaaagcgggc
     2161 cagcggatca agatcaaatc aaaaacctaa aacgtccgac tggtaatgat taaagctgct
     2221 tacgtcaata tttggaacaa gcgtgtgggt gcagttgcgt ggaatgagga agaagggata
     2281 gctactttca gatatgaccc cgaattcctt gaaaccggcc tggatttatc acctgtaatg
     2341 atgcctttga atgaatctct gaattacagg tttccggaac atcggaacac aacaacgttt
     2401 aaaggtttgc ctgggatgct cgcagacgtg ctgccagaca agtacggaaa tgcactaata
     2461 aatcaatggc tagtgaagca caacaggccg gccaatagca ttaaccccgt tgaaaccctg
     2521 tgcttcattg gtaaaagagc gatgggcgca ttggagtttg aaccttctga acctaagata
     2581 acaggccact ccattaagct tgaaatgaat gaattgattg acattacatc aaagatactt
     2641 tcaggcagac aagcgtttct aaccaatctc cagccggatc aggaaaaagc cttacaagca
     2701 attctaaaaa tcggaacgtc tgccggtgga gcacgagcaa aggcattgat cgaattcaat
     2761 aaggagacta aggaaatcag aagtggtcaa gctaatgctc ctaagggatt tgatcattac
     2821 ctgatcaagt ttgatggaat tgaagatgag cagtttggta catccagagg gtatggacga
     2881 gtggaaatgg cctactacaa aatggctgtt gatgcaggaa ttgaaatgat ggaaagtgag
     2941 ttacttgaag aaaatggccg ggcacacttc atgaccaaaa ggtttgaccg ggtacaggga
     3001 aagggtaaaa ttcatgttca gtctttttgt gcaatgcgtc actatgactt caaagacatt
     3061 gatatatact cttatgaaga cttgtttgaa acaatgagac ttcttgtatt gccataccct
     3121 caggcagagc agctgttcag gagaatggtg ttcaatgtta ttgctcgaaa ctgcgatgac
     3181 cacaccaaga atttcgcctt caccatggat aaaagtggag aatggtcact ttctccagcc
     3241 tttgacgtgt gtcatgccta tcggccatcc agtacatggg tgagtcagca ttgcctgagc
     3301 gttaatggta aaaggaaaaa cattgaacga gccgacttgc tcgaagtggc aagaaaaatg
     3361 aatattaaag gagcaagtga gatcattgat caggttgacc aggtgatcgg gaagtggaaa
     3421 aaatatgcgg atgatcagaa agttgatcct gagttaaggg aagctatcaa gagtacattt
     3481 gttagaatga ataaggccag gacatgaagc ttcaaatgat agagtgcagt gatcttacat
     3541 cgcggcagct actacctctg cccatatgac aaaatattca aattgatcat tgctcattca
     3601 gatggagcaa aaaagaatgg agaataagta tctattgtat tcctgcatgg cttgttcctc
     3661 atctggagaa atataaattg atcttatgat gtacccaatc ggtaccttgt gcacatattt
     3721 gccatattgt ctatagtatt tatatgttgt atccggaagt gatcgacaca agagcagatg
     3781 tttctgaata tagaaatatc tcaggatggc atattggtta cacttggaag ttcgataggt
     3841 gtaggaacat cagatacttt agggcatcat gaacccgtat ttttagtatt aatgatgttt
     3901 atataaattg ccggtgttta tgccatattc aggaggatcc tctggtggcg ttccaaagta
     3961 ctcttcgtgc aaacagcagc aggtaaccca acaggatata ctccaggaac ctgatggcca
     4021 tcacagcaaa gatgttcagc cgtttgatcg gttctccgtt gtcatcatag cagacgaaat
     4081 cctgttcgat gtaagtggag cctggtaccg ggttggaaac attctcttta cgggtcaatg
     4141 taccaccatc acacatggtc atctccaccg gtttgttgta cagatagttg ccggtctgat
     4201 cgacagagat gcccatcagg gcaaaaaagg caatacctaa agcccgtgtg aatccggaag
     4261 tattctgtgg ctttctgagt ggtgttacac cgaacccgat cataccggct atcagcatga
     4321 ccgcactggc cactccttct atggtggcaa tggcgacaaa ggatccgata aagaatgcga
     4381 tcaggccgat cacgccaacg accaatatca gtatccatcc ccaggaagga atgcgtttcc
     4441 accgggacgt cgctggtggt ttcgagaagg ccatggagtt gccacagtga ttgcagtaag
     4501 atgcatcatc cggattctcc ttcccgcagt gcgagcatgt tctcataact ctgtagcagg
     4561 ctgatgcttg aacttaccct tgctcttcct gatcctgcag gctaccactt tgtattccgg
     4621 acacatggct tcgctgtcat gtacatcgct tgtgatgttg ttcagcatga tgtcggggaa
     4681 gtggaaggtg gaactcagta cacccggacg aacttcatcg gtgatccggg ctttgatgtc
     4741 cactttgccc cgcggacttt ccacacagac catatcacca tcggcgatac cgttggcagc
     4801 tgcatcgtcc ggatggatca tcagtgtatc ttccgtcagg atctgtacgt tgcgggtgcg
     4861 acgggtcatc gtaccggcat tgtaatgttc cagttcgcgg ttggtggtga tgatgtatgg
     4921 atagtctttc ccattcgcat tgatctcttc actctctctc catggttcga aatggaattt
     4981 gcccttaccg cgtttgaagt cttcggtatg caatatctct gttcctgtgc catcgggttt
     5041 caccggccac tgcttgcctt gttcacccag ctcatcccac ttcacacctg cgaagaaagg
     5101 cacgatctga cttacttctt ccagtaccca gcgaggatgg taatcaggct gcgggaagcc
     5161 catatgggtc attacctcac atatgatctg tccgtcaggc ttggtgccgg gcaagggttc
     5221 taccactgca ttcacccgct ggatgcgacg ttctccattg gtgaaggtcc cgctcttttc
     5281 caggaaggat gaagcaggga aaatgacatc ggcatgcttg gtcgtttcgg tatggaagat
     5341 ctcctggatc accagcaggt cgaggttgtc gagcgccttc atcacccgct gggtgttcgg
     5401 gtcggtttgt accacatctt cgcccatcag ccacagagct ttcagattgc cgttcagcgc
     5461 ttcatcccac atctccggaa ttttcaatcc cttacctatc
//
LOCUS       DAOMAX010000177         5482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2459_length_5482_cov_4.157914, whole genome shotgun sequence.
ACCESSION   DAOMAX010000177 DAOMAX010000000
VERSION     DAOMAX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2459_length_5482_cov_4.157914"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK742_09250"
     CDS             <1..428
                     /locus_tag="PK742_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQU76814.1"
                     /translation="KWIEKAKDKNDPFRLMGFGHRVYKNFDPRAKIIKEACDNVLNKL
                     GIHDPILDIAKQLEEVALQDPYFVEKKLYPNVDFYSGIIYRAIGFPTEMFTVLFALGR
                     LPGWLAQWKEMREAGQPIGRPRQIYTGEVKRDYTNIATR"
     gene            700..1074
                     /locus_tag="PK742_09255"
     CDS             700..1074
                     /locus_tag="PK742_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQU76815.1"
                     /translation="MRTTSNEILIYYDPEQTTARQVLAYARTLSSHVHEVEYHKTKFS
                     TTIWRQILHMLGDPEPKSLLNKAHPFYQQHLRGKDYNREDILNIIIRNPGIIRAPIAI
                     KGRRAVFCEQPTDILRLGALAA"
     gene            complement(1071..1472)
                     /locus_tag="PK742_09260"
     CDS             complement(1071..1472)
                     /locus_tag="PK742_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76816.1"
                     /translation="MRSAGYFWIVAGILSLSVSCNRSSDNSETYEQGGAALKNMSKVD
                     PEDPFSGQPNNLCTVIRILPATDIAEPEDKYCLLDVCLDADEESLLTVDLGDGEDHAM
                     AAYKLIKVFSTADEARQYAAQYQIIDVLITD"
     gene            complement(1469..1972)
                     /locus_tag="PK742_09265"
     CDS             complement(1469..1972)
                     /locus_tag="PK742_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HQU76817.1"
                     /translation="MENERLSAANTYRKKISNGPTFKLFMFVKMPIAWLSGLKIISLD
                     QNECTVSIPYKRLTQNPFRSVYFASQAMAAEMSTGVLAMAAIQGIRPSVSMLVANVEA
                     AFHKKADSRIFFTCKDGATFMQTVDEAVQSGEGRTATGISEGFLADGTHVSTFKVTWT
                     FKQKKSK"
     gene            2037..2315
                     /locus_tag="PK742_09270"
     CDS             2037..2315
                     /locus_tag="PK742_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HQU76818.1"
                     /translation="MGAFFTDERTETTVSVEDLLGRHLVVHNDEVNTFDWVIQSLVEI
                     CRHSVEQAEQCAMIIHFKGKYAVRQGQDDTLRPMREALVDRGINATIE"
     gene            2312..2965
                     /gene="aat"
                     /locus_tag="PK742_09275"
     CDS             2312..2965
                     /gene="aat"
                     /locus_tag="PK742_09275"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQU76819.1"
                     /translation="MNRPVILQTTDPFPFPYHSDADGLVAIGGGLSVRRLLAAYSIGL
                     FPWFEEDGWPFWFSPDPRCVLIPAQLKVSGSMRVLFNKQAFRVTFDQDFEAVIRNCAE
                     VHRTGQEETWISEHFIKAYRRLHKAGYAHSVEVWDDHEQLVGGLYGVQIGKVFFGESM
                     FSKQSNASKYGFISVVRKLQKEGVELIDCQQETRHLKSLGAGTISRSTFLSALQRLV"
     gene            complement(3002..3628)
                     /gene="pdxH"
                     /locus_tag="PK742_09280"
     CDS             complement(3002..3628)
                     /gene="pdxH"
                     /locus_tag="PK742_09280"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQU76820.1"
                     /translation="MDIAGMRENYRKGALDMDHTDPLPITQFRQWFDEAVQSAIEEPN
                     AVILATSTPDGHPSVRTMLLKGVEDNGFVFFTNKHSRKGREMADNPFASLLFFWGALE
                     RQVRIEGRLTALSEADNDAYFYSRPIESQFGAMASPQSQPIESRSWLEKRMEELQAMN
                     KPVRPAHWGGFLLEPDVMEFWQGRPGRLHDRIQYVLTDGVWIRQRLAP"
     gene            3747..4310
                     /locus_tag="PK742_09285"
     CDS             3747..4310
                     /locus_tag="PK742_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU76821.1"
                     /translation="MNLKHLILVCVMLCSFSASAQLIQLSGIVAEDRTGYPLPFATVH
                     VLGTYRGTVSSANGFFSLVVAPNDSIRFSSVGYESRLFVVPDTLTDVITSIGVFLPID
                     TMQLETVEVYPWPTRDDFADAFLALQLANHELRMGPIPGIKTVVDTVPPAPTIFNPIT
                     LFYEEIIKPIEWNRVKRQKSQELPRWD"
     gene            complement(4313..>5482)
                     /locus_tag="PK742_09290"
     CDS             complement(4313..>5482)
                     /locus_tag="PK742_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76822.1"
                     /translation="DNPVWYFVSGMLAAVIFLQRAFIVVQPTIGEIRKSLTRYLHWLV
                     FVAGAVIVGNLFSELITVYPLDMLDTSFSDIYPQVHIMTDRFLHGEKVYADIPFNGYT
                     LFPTYLPLTWMPFIPAEILGLDYRWVSYTICLAGILVYAIWLHRSAIAWWQKSLYMVL
                     PFAGWLSYVWYHQQETAYSLESMIAGFYMLLLFSLFSKRPAVRILALMLCLLSRYSVV
                     LWIPLYILVMYRESGKMAWRMTVYGVVILLVLYVIPFLIHDPSIFQQGYAYHDKAALA
                     EWQPSEPDQIPGKHLHEGAGLAIFFFKYGKGEMDHRLQTLQQVHLLLTSMIVLLCGVL
                     YLRFRPDKDLFLAGSLILYLTFFYHLIQIPYSYLFLTLLLMGSVLPVLLLRKSIS"
BASE COUNT         1458 a         1298 c         1308 g         1418 t
ORIGIN      
        1 ccaagtggat cgagaaggcc aaagacaaga acgacccgtt ccgcctgatg ggctttggcc
       61 accgggtcta taagaatttt gatccacggg cgaagatcat caaagaggct tgtgataatg
      121 tgctgaacaa attaggcatc cacgatccta tcctcgacat tgccaagcaa ctggaggaag
      181 tggccctgca ggatccttat ttcgtagaga agaaactcta tccgaatgtg gacttctaca
      241 gtggtatcat ttaccgggcc atcggattcc cgacagagat gttcacggtg ctctttgcgc
      301 tgggtcgcct gccgggctgg ctcgctcaat ggaaagagat gcgtgaggcc ggacaaccga
      361 tcggcaggcc acgccagatc tataccggcg aagtcaagcg ggattatacc aatatcgcca
      421 ccaggtgatc atttctggaa ttacgataca ggtcgccgat tggcggcctt ttttttgccc
      481 catttgtact ggccggccag tttctcctgc atgctgaatt tgcatcaggc acccgatttc
      541 tacacgccat gaaatggaaa aattcgccat tcaggtaacc tcaacgagga aaatggtcat
      601 tattaaaaag gaatcaaata cgtttgtgca attaaaaaat attgaacaat ttgacaattt
      661 tatgccaatt gattagttta atgggcaaat cattgtcata tgcgaaccac ctccaacgag
      721 atcctgatct attatgatcc ggaacaaacc actgcccgcc aggtactcgc ctatgcaaga
      781 accctctcgt cgcatgtaca tgaagtggag taccacaaaa cgaaattctc caccaccatc
      841 tggcgacaga tcctgcatat gctgggagat ccagaaccta aatcgctgct gaacaaagcc
      901 cacccgtttt atcagcaaca cctgagaggt aaggattaca accgggaaga tattctgaac
      961 atcatcatcc ggaatccggg aattataagg gcgcccattg caataaaagg caggcgggcg
     1021 gtcttctgtg aacagccaac cgatatactc cgccttggtg cactggcagc ttaatcggtt
     1081 atcaatacat caatgatctg atattgtgct gcatattgac gtgcttcatc ggctgttgaa
     1141 aagactttga tgagcttgta ggcagccatg gcatggtctt ctccatcgcc aagatcaact
     1201 gttagcaggc tttcttcgtc tgcatccaga caaacgtcca gcaaacagta cttgtcttct
     1261 ggttcagcaa tatctgtagc aggaaggatg cgaatgacgg tacaaaggtt atttggctga
     1321 ccactgaacg gatcttccgg atcaacttta gacatgttct tcaaggcggc gccaccctgt
     1381 tcataggtct cactattgtc agaactgcgg ttgcagctga cggagaggga taatattccg
     1441 gctactatcc aaaagtatcc tgctgatctc atttgctctt tttttgtttg aacgtccatg
     1501 ttaccttaaa cgtactaacg tgggttccgt ctgccagaaa tccttcagag atccctgtag
     1561 cagtgcgccc ttctccgctt tgtactgcct catcaactgt ctgcatgaat gttgctccgt
     1621 ccttacaggt aaaaaagatc ctgctgtcag cttttttgtg aaatgccgct tccacattag
     1681 ccacaagcat cgaaacagac ggtctgatac cctgtatggc tgccatggcg agtaccccgg
     1741 tactcatttc tgccgccatg gcctgtgaag caaaatagac agaacgaaaa gggttttgtg
     1801 tcagtctttt atacggaata ctcacggtgc actcgttctg atccagcgag atgatcttca
     1861 gtccggacag ccaggcaatg ggcattttga caaacataaa tagtttaaat gtaggcccgt
     1921 tggagatctt ttttcggtat gtatttgcgg cagaaaggcg ttcgttttcc atggatttca
     1981 aaagtaaggt tttttcattc cccgattttc gctataattt tgtagcaaat tcaggcatgg
     2041 gcgcattttt tacagatgaa aggaccgaga ccactgtatc ggtggaagat ctgctgggtc
     2101 gccacctggt ggtacacaac gatgaagtga atactttcga ttgggtcata cagtcactgg
     2161 tagagatctg ccgccactct gtggagcagg ccgagcagtg tgccatgatc atacatttca
     2221 aaggcaaata tgccgttcgt caggggcagg atgatacttt gcgtccgatg cgggaagccc
     2281 tggttgacag aggtatcaat gctaccattg aatgaaccgt ccggtcattc tgcagacaac
     2341 cgatccgttt ccttttccat accattcaga tgccgatggc ctggtagcca ttggtggagg
     2401 attatctgta agacgattgt tggcagccta ttctatcgga ctgtttccct ggtttgaaga
     2461 ggacggatgg cccttctggt tttctcctga ccccagatgt gtgctgatac ccgcacagct
     2521 gaaagtgagc ggcagtatgc gggtgctgtt caacaaacag gcgttccgtg taacgttcga
     2581 tcaggacttt gaagcggtca tcagaaattg tgctgaggta caccggaccg gacaggaaga
     2641 gacctggatc agtgagcatt tcattaaagc atataggcgc ctgcacaagg caggatatgc
     2701 ccactctgtg gaggtttggg acgatcatga gcagcttgtt ggcggtttgt acggtgtcca
     2761 gatcgggaag gtcttttttg gcgaaagtat gttcagtaag caaagcaatg ccagtaagta
     2821 cgggtttatt tcagtggtca gaaagttgca gaaggagggt gttgaactga tcgattgtca
     2881 gcaggagacc cgccatctga aaagcctggg tgcaggtaca atatcacgaa gtactttttt
     2941 atcagctctg cagagactgg tataatgatg accggttcat agcaggtgcg ctgttcaaat
     3001 ttcagggtgc cagtctttgt ctgatccaga caccatctgt aagtacatat tgtattctgt
     3061 catgcagccg gccgggcctt ccctgccaga attccatcac atccggttcc agtaagaatc
     3121 caccccaatg ggcaggccta acaggtttgt tcattgcctg cagctcttcc attctctttt
     3181 ccagccagct ccggctttct atcggctggc tttgaggtga tgccatggca ccaaactgag
     3241 actcaatagg tctggaatag aaataggcat cgttatctgc ctcactgagt gctgtaagcc
     3301 gcccttcaat acgcacctgt ctttccagag caccccagaa gaacagcagg ctggcaaacg
     3361 gattatcagc catttcccgg ccttttctgc tatgcttatt ggtaaaaaaa acaaagccgt
     3421 tatcttccac acctttgagc aacatggtgc gaacacttgg atgtccgtcc ggagttgatg
     3481 tggccaggat tacagcattc ggctcttcaa tggctgattg tacggcttca tcaaaccatt
     3541 ggcgaaactg tgtgatgggt aatggatccg tatgatccat gtccagtgca ccttttcggt
     3601 agttctctcg cattccagca atatccatgg ctaaactttt caccaaaaat acggtcaaag
     3661 accggccaat atgatgattt atgtatccat tgggttaaga ttggtgaact ttatgccatt
     3721 gaacaatatt ttacgttcat aaccggatga acctgaaaca cctgatactg gtctgtgtaa
     3781 tgctgtgttc tttttctgca agtgcacaac tgatccagtt atccggtatt gtggctgaag
     3841 accggaccgg atacccgctg ccttttgcta ccgtgcatgt gctgggtacc taccgcggaa
     3901 ctgtcagcag tgccaacggc tttttttcac tggtggtagc tccaaacgac agtatccgtt
     3961 tttcttcagt gggttatgaa agcaggttgt ttgtagtgcc agataccctg accgatgtga
     4021 tcacttccat tggtgtgttc cttcccatag ataccatgca gctggaaacg gtcgaagtgt
     4081 acccctggcc caccagagat gattttgcag atgcttttct ggcgcttcaa ctggcaaatc
     4141 atgaactcag gatggggcct attcccggaa ttaaaacggt ggtggataca gtgcctcctg
     4201 caccaacaat attcaatccc atcacactgt tctatgaaga gatcatcaag cccattgaat
     4261 ggaatcgtgt gaaacgacaa aaatcacagg aacttcccag gtgggattaa tctcagctaa
     4321 tactctttct caacaaaagt accggcagga ccgaccccat taacagtagg gtgagaaaca
     4381 aataagaata aggtatctgg atcagatgat aaaagaaggt caggtataat atcagacttc
     4441 ccgccaggaa aaggtctttg tcaggcctga atcgcagata gagtactccg caaagcaaca
     4501 cgatcatcga agtaagcagt aaatggacct gttgcagagt ttgaagtctg tggtccattt
     4561 ctcctttacc gtacttgaaa aagaaaatag ccagacctgc tccctcgtgc agatgttttc
     4621 ctggtatctg atctggttct gacggctgcc attctgccaa agctgccttg tcatgatagg
     4681 catagccttg ctgaaaaatt gaaggatcat ggatcagaaa aggtataacg tacaaaacca
     4741 acagtatcac cacaccatat acggtcatgc gccaggccat cttacctgat tccctgtaca
     4801 tgacaaggat gtacagtggt atccataaga ccacagagta gcgtgaaagc aggcaaagca
     4861 ttagtgccag tattcttact gctgggcgtt tgctgaacag actgaacagc agcagcatgt
     4921 agaatcctgc tatcatgctt tccagcgaat aggcagtttc ctgctggtga taccagacat
     4981 aggaaagcca ccctgcaaaa ggcaacacca tatacaggct tttctgccac catgcgatcg
     5041 cagaacggtg aagccatatg gcatatacga ggatacccgc gagacagatg gtgtatgaca
     5101 cccatcggta gtccagtcca agtatttctg caggaatgaa aggcatccag gtgagtggca
     5161 gataagtggg gaataaggta tatccgttga aagggatatc tgcatacacc ttctcaccat
     5221 gtaaaaatct gtcggtcatg atatgcacct gcggatagat atcagagaat gatgtatcca
     5281 gcatatcaag ggggtaaacg gtgatcaact cactaaacaa gtttcccaca atgacggccc
     5341 cagcaacaaa gaccaaccag tgcaggtacc tggtaagaga ttttctgatc tctcctatgg
     5401 ttggttgtac cacaatgaaa gcccgttgca ggaagatcac agcagccagc attccggaaa
     5461 cgaaatacca gacgggatta tc
//
LOCUS       DAOMAX010000178         5462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2472_length_5462_cov_3.723137, whole genome shotgun sequence.
ACCESSION   DAOMAX010000178 DAOMAX010000000
VERSION     DAOMAX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2472_length_5462_cov_3.723137"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK742_09295"
     CDS             <1..265
                     /locus_tag="PK742_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76823.1"
                     /translation="GYAVTTSMHGEERAFVMSGQYEQVLPMDIYPVHLLKAIMARDFD
                     LMEGLGIYEVTEEDFALCEFVCTSKIDVQEILREGLDYMRSQN"
     gene            287..1525
                     /locus_tag="PK742_09300"
     CDS             287..1525
                     /locus_tag="PK742_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HQU76824.1"
                     /translation="MDPLKPIRNSVGKKLWKQVEELEPSFKKDGMLHSLHSTFDAFKT
                     FLFVPPTVTSKGAHIRDAIDLKRTMFLVIIAMIPALLHGIFNVGHLHYVANGMYPHFF
                     DGFGPKLVYGLIHVLPIVIVSYVVGLGIEFAFAQFRGHPVNEGFLVSGMLIPLVMPPD
                     IPLWMVALATAFSVIIVKEAFGGTGMNILNVALTARVFIFFAYPTYISGDQVWIAGLP
                     ESPDAFSGATVLGVWAKQGYDAMSHVGFSTVSQQVGYTPMDMFLGFIPGSIGETSKLA
                     ILLGAVFLIWTGIGSWRIMLSVFVGAFLMGLILNAAALGFDLSNPHLAVPAYYHLLAG
                     GLAFGAVFMATDPVTATQTTTGKYIYGLLVGMISILIRVLNPAYPEGVMLAILFMNVF
                     APLIDHMVIQSNIKRRLARG"
     gene            1518..2234
                     /gene="nqrC"
                     /locus_tag="PK742_09305"
     CDS             1518..2234
                     /gene="nqrC"
                     /locus_tag="PK742_09305"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HQU76825.1"
                     /translation="MDRNSNIYTIIYAAILTVVVAVVLAFVHSSLEPKMEANEKLAKQ
                     KDILRSVGLVDLPDASAVYEKTISSFVIDASGKVVDGMDGEAVNVAAEFKKPAEDQVH
                     PVFVYTADDGSKRLILPLYGNGLWDKIWGYIALEEDMNTIAGIAMDHKGETPGLGAEI
                     KDNPKQYNDPFIGKTILDEQGNYVSVKMVKGGIKEPEHQIDAISGATITSDGVNEMLY
                     RGLGYYLPYFNAAKSQTSLK"
     gene            2240..2887
                     /locus_tag="PK742_09310"
     CDS             2240..2887
                     /locus_tag="PK742_09310"
                     /inference="COORDINATES: protein motif:HMM:NF006777.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HQU76826.1"
                     /translation="MSETQVAQKSTTLFGKKERRLLTDPFNDNNPITVQVLGICSALA
                     VTVAVKNALVMAVAVTLVTGFSNLIISLLRNTIPNRVRMIVQLVVIASLVSIVEMVLH
                     AFQYDVYKQMSVYIGLIITNCILMGRLEAFAMANKPWQSLLDGIGNGVGYGAILIAVA
                     FFRELFGKGSVFGFRVLPDWYVPNGLMLIPAAAIFLIGIIIWIQRSRNTKLVDIS"
     gene            2907..3497
                     /gene="nqrE"
                     /locus_tag="PK742_09315"
     CDS             2907..3497
                     /gene="nqrE"
                     /locus_tag="PK742_09315"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HQU76827.1"
                     /translation="MEYINIFIKASFVENMVLSFFLGMCSFLAISKKVKTAVGLGAAV
                     VFVLTMTVPINWLISNYLLSENGIFHTDLTFLKLIIYIAVIAAMVQLVEMVVERFSPA
                     LYSALGIFLPLITVNCSILGGALFMDSREYELGEAAVFGFGSGFGFFLAIVAIAAIRE
                     KLRYSNIPAPLRGLGIAFIITGLMGLAFMSFIGIKL"
     gene            3507..4772
                     /gene="nqrF"
                     /locus_tag="PK742_09320"
     CDS             3507..4772
                     /gene="nqrF"
                     /locus_tag="PK742_09320"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HQU76828.1"
                     /translation="MNWTLLLDSSVIIYSIGVFAGVILLLTVTLLFAKSKLTPAGNVN
                     IVINGENDKPVVTTPGGTLLSTLSQQHIYLPSACGGGGTCAMCKCQVIEGGGDILPTE
                     EGYFTRKQVQDNWRLACQVKVREDMQIKVPEEVFGIKKWECEVVSNHNVATYIKEFVV
                     KLPPGETLHFESGGYIQIDVPAIEVKFKDMVVEKEYHDEWDRYKMWDLVMKNDEPIYR
                     AYSMANHPAEGNIVMLNIRIATPPWDRVKGQFMDVNPGICSSFIFSRKPGDKVMVSGP
                     YGEFFIKPTNKEMVYIGGGAGMAPLRSHIFHLFHTMKTDRKVSYWYGGRSTRELFYTE
                     QFQEIEKQFPNFSYHIALSDPTPEDNWTGPVGFIHQVVLDNYLKNHPEPEEIEYYLCG
                     PPQMNAAVMKMLDNLGVPNENIAFDDFGG"
     gene            4829..>5462
                     /locus_tag="PK742_09325"
     CDS             4829..>5462
                     /locus_tag="PK742_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQU76829.1"
                     /translation="MRHLLISFILLSLTACDRSSLTEFTVRGETMGSTYSIIYLDSAG
                     RDLGTALDSMLVEFNAVFSTYDTSSVISAFNASDTGIALIGEQLEWFRRLFAVSDEVH
                     AIHNGAFNPAIGPLVQYWGFYNKQESLEAEPPQDEIDSLLAISRYELVHLDQQFLVKD
                     DPAVRLDVNAIAPGFAADLLGEFLEANGIQRYMVEIGGEIRARGLNSRNTG"
BASE COUNT         1335 a         1220 c         1379 g         1528 t
ORIGIN      
        1 cggttatgcg gtcactactt ccatgcatgg agaagaaaga gcctttgtaa tgtcaggtca
       61 gtatgaacag gtgttgccta tggacattta tcctgtgcac ctcctgaagg ccatcatggc
      121 ccgtgatttt gatctcatgg aggggcttgg tatttacgaa gtgactgaag aagattttgc
      181 tttatgtgaa tttgtttgta cctcaaaaat tgatgtacag gagattctgc gtgaaggtct
      241 ggactatatg cgttctcaaa actgataaac aggaaaatca acattgatgg atcctttaaa
      301 acccatacga aattctgtag gcaaaaagct gtggaagcag gtggaggaac ttgagccgag
      361 tttcaaaaaa gacggcatgc tgcattcgct tcattccaca ttcgatgctt ttaaaacctt
      421 tttgttcgta ccaccaaccg taacatccaa aggagctcat attcgcgatg ccattgatct
      481 gaagcgtacc atgtttctgg tcatcattgc catgattccg gcattgttgc atggcatttt
      541 taatgtaggt catctgcatt atgtggccaa tggtatgtat ccgcatttct ttgatggctt
      601 cgggccaaaa ctggtatatg gactgatcca cgtgctgccc atcgttattg tcagttatgt
      661 ggtgggtctt ggaatcgaat ttgcctttgc acagtttcgc ggccatccgg taaatgaagg
      721 ctttctggtt agtggtatgc tgataccgct ggtcatgcca ccagatattc cgctttggat
      781 ggtggcactg gctacagcct tctctgtcat cattgtgaaa gaagcatttg gtggaaccgg
      841 tatgaatatt ctgaacgttg cgcttactgc aagggtcttt atattctttg catatcctac
      901 ttatataagt ggagatcagg tctggatagc cggcctgccg gaaagcccgg atgcattttc
      961 aggagctacc gtgctgggcg tatgggccaa acaaggctac gatgctatga gtcatgtagg
     1021 tttcagtaca gtttcacaac aggtgggtta caccccgatg gatatgttcc tgggtttcat
     1081 tccgggatcc ataggcgaga ccagcaaact ggccattctt ctgggtgccg ttttcctgat
     1141 atggacagga atcggtagct ggcgaatcat gttgtctgtg tttgtaggag ctttcctcat
     1201 gggactgata ctgaatgcag cggctcttgg gttcgatctc agcaatccgc atctggctgt
     1261 tcctgcctat taccatcttc tggcaggagg cctggcattc ggagccgtat ttatggcaac
     1321 ggatccggta actgctaccc agaccaccac cggaaaatat atttatggtc tgctggtagg
     1381 aatgatatcc attctgatca gagtgctgaa ccctgcatac ccggaagggg tgatgctggc
     1441 catcctgttc atgaatgtat ttgcaccact tattgatcat atggtcattc agtctaacat
     1501 taaacgccgt ttagcccgtg gataggaata gtaacatcta tacgatcatc tacgctgcca
     1561 tcctgaccgt ggtggtggcc gtagtgctgg cttttgttca cagcagcctg gaaccaaaga
     1621 tggaagccaa tgaaaaactg gccaagcaga aagatatcct ccggtctgtt ggattggtgg
     1681 atcttccgga tgcaagtgct gtgtatgaaa agaccataag cagttttgtg atagacgcat
     1741 ccggaaaagt tgtggatggt atggatggtg aagctgtgaa tgtagccgcc gaattcaaaa
     1801 agcctgcgga agatcaggta catccggtat ttgtctatac ggccgatgac ggcagcaaac
     1861 gtctaattct gcccttatat ggtaatggtc tttgggataa gatctgggga tatattgctc
     1921 tcgaggaaga tatgaatacc attgccggta ttgccatgga tcacaaaggt gaaacaccgg
     1981 gtctgggtgc agagatcaaa gataatccga aacaatacaa cgaccctttt attggcaaga
     2041 ccatcctgga cgaacaaggt aactacgtat ctgttaagat ggtgaagggc ggtataaaag
     2101 aacccgaaca ccagatcgat gccatttcag gtgccactat cacaagtgat ggtgtgaatg
     2161 agatgctgta ccgtggcctg ggttactacc tgccttattt caatgctgcc aagtctcaaa
     2221 cttctttaaa ataaacgcca tgagtgaaac acaggttgct caaaaaagta ccacgctgtt
     2281 cggcaagaaa gagcgcagac tgctaactga ccccttcaat gacaacaacc ctatcactgt
     2341 gcaggtgttg ggtatttgca gtgcactggc tgtaaccgtg gctgtcaaga atgccctggt
     2401 catggcagtg gcagtgaccc ttgtaacagg attctccaac ctcatcattt ccctgctgcg
     2461 taataccatc cccaaccggg tgcgtatgat cgttcagctg gtggtcattg catcactggt
     2521 ttccatcgta gagatggtgc tgcatgcctt ccagtatgat gtgtacaagc agatgtcggt
     2581 gtatatcggc ctcatcatca ccaactgtat cctgatggga agactggagg cattcgccat
     2641 ggctaacaag ccctggcaat ctttacttga tggcattggc aacggtgttg gttatggtgc
     2701 cattctgata gccgtagcct tcttccgtga actgttcgga aaaggatccg tgttcggctt
     2761 ccgcgtgctg ccagactggt atgtgcctaa tggactgatg ctgataccgg ctgctgccat
     2821 tttcctcatc ggtatcatca tctggatcca gcgttcacgc aatactaaac tggtagatat
     2881 ttcctgacct gtctcaatac tgcaacatgg aatacatcaa tatattcatt aaagcctcct
     2941 tcgtggagaa catggtactg agtttctttc tcggtatgtg ttccttcctg gcaatttcaa
     3001 agaaggtaaa gacagcagtt ggtcttggag ccgctgtggt ctttgtactg accatgaccg
     3061 tcccgatcaa ctggctcatc agtaattatc tgctcagtga gaacggtatt tttcatacag
     3121 atctgacctt cctgaagctg atcatttaca ttgcggtgat cgcggcaatg gtgcagctgg
     3181 tagaaatggt agtggagcgt ttcagcccgg cactgtatag tgctctgggt atcttcctgc
     3241 cacttattac cgtgaactgc tccatccttg ggggcgccct tttcatggac tccagagaat
     3301 atgaactggg tgaagctgct gtgtttggat tcggctccgg tttcggattt tttctggcta
     3361 ttgttgccat agcagccatc cgcgaaaagc tgcgctattc caatattcct gcaccgcttc
     3421 gcgggctggg tatcgcgttt atcatcacag gtctgatggg cctggccttt atgagtttta
     3481 tcggtatcaa actgtaatct ttcgtcatga actggacctt attgcttgat tcgagtgtca
     3541 ttatttacag cattggcgtc tttgcaggcg tcatcctgtt actgaccgta accctgcttt
     3601 tcgccaagtc aaaactgact cctgctggta atgttaacat cgtgatcaac ggtgaaaatg
     3661 acaaacctgt ggtgaccact cctggtggaa ctttgctgag tacacttagc cagcagcata
     3721 tttatctgcc ttcagcctgc ggtggtggtg gtacctgcgc catgtgcaag tgtcaggtca
     3781 ttgaaggtgg cggagatata ctgcctactg aagaaggata ctttacccgt aaacaagttc
     3841 aggacaactg gcgtctggca tgtcaggtaa aggtgcgtga ggatatgcag atcaaagtac
     3901 cggaagaggt tttcggtatc aagaaatggg aatgcgaagt ggtttccaat cacaatgtgg
     3961 ccacctatat caaggagttt gtagtgaaac ttcctccggg cgaaacactg cattttgaaa
     4021 gcggtggata tattcagatc gatgtacctg ccattgaagt gaagttcaag gatatggtgg
     4081 tagagaaaga ataccatgac gaatgggatc gctacaagat gtgggatctg gtcatgaaga
     4141 atgacgaacc aatttaccgt gcctattcta tggccaacca ccctgctgaa ggaaacatcg
     4201 tgatgctgaa catccgtatt gccacaccac cctgggacag ggtcaaaggt cagttcatgg
     4261 atgtgaatcc cggaatctgt tcttctttca tcttctcccg caaacctgga gataaggtga
     4321 tggtgagcgg cccttatggt gagttcttca ttaagccaac caacaaggaa atggtatata
     4381 ttggtggtgg tgcaggtatg gcaccgctgc gttctcatat cttccatttg ttccacacta
     4441 tgaagaccga ccgaaaggta tcttactggt atggtggtcg ttccactcgt gagttgttct
     4501 acacggaaca gttccaggaa atagaaaaac aattcccgaa tttcagttac cacattgctt
     4561 tgtctgatcc aactccggaa gataactgga caggccctgt agggttcatc caccaggtgg
     4621 tgctggataa ttatctgaag aaccatccgg aaccggaaga gatcgagtac tacctgtgtg
     4681 gtccgccgca aatgaatgca gctgtcatga agatgctcga taatcttggc gtgccaaatg
     4741 aaaatattgc cttcgacgac ttcggcggct gatcttaaat tacttttcac tgcccctccc
     4801 tttgcggagg ggcttttatt tttataccat gcgccatctg ctcatctctt ttatattgct
     4861 atcactgaca gcctgcgacc gctcttcgtt gaccgaattc acagttcgcg gcgaaaccat
     4921 gggttctacc tacagcatca tctatctgga ttctgccgga cgagatctgg gtacagctct
     4981 cgatagtatg ctggtggagt tcaatgcggt attcagtact tatgatacca gctctgtgat
     5041 cagcgccttc aatgccagcg ataccggaat tgctcttatt ggcgaacaac tggagtggtt
     5101 cagaaggttg ttcgcggtat ctgatgaagt acatgccatc cataacggtg cgttcaatcc
     5161 tgctattggt ccgctggtgc agtactgggg tttttacaac aaacaggaat cgctggaagc
     5221 tgaacctcct caggatgaga ttgacagttt gctggccatc agccggtatg aactggtgca
     5281 tctggatcag cagtttctgg tgaaggatga tcctgccgtg cgcctggatg tcaatgccat
     5341 tgcgcccggg tttgcagccg atctcttagg agagtttctg gaagcaaatg ggattcaaag
     5401 atacatggtc gaaataggcg gtgagatcag ggccaggggt ttgaacagtc ggaatacagg
     5461 ct
//
LOCUS       DAOMAX010000179         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2512_length_5405_cov_4.126542, whole genome shotgun sequence.
ACCESSION   DAOMAX010000179 DAOMAX010000000
VERSION     DAOMAX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2512_length_5405_cov_4.126542"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PK742_09330"
     CDS             complement(<1..1084)
                     /locus_tag="PK742_09330"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU76830.1"
                     /translation="MRSVWLLLTTSLFSVQLQAQVVNTFGDWMPVNLETDTTVIYLAD
                     YFEKPTMVTGVEAPPRMQARLDPLKQQVTIVPGQDIPPLSVLTFHTGEQEWSLVLRRS
                     KKEEVSFIFNPGGVAYLDVSLAGDMNSWNPDATPLEYHNGQWQTRLWLNPGRYQYQIV
                     ADTSWMLDPNNPVQVPNGIGGINSLLVVGDTSFAGRPYFYLDKTEDNKIVIGSENRNA
                     LDPVFVLINNHLIPFSEGTSLSFVMPEEMAGQPEGSIRVFGYNEVNEGNDMMIPVSFG
                     KVVEDAALINRHDFHKAIVYFLLVDRFYNGDKTNDAPVDDPRVDPKANYMGGDLAGVQ
                     QKLQDGYFTNMGFNTLWLSPVMQNPHV"
     gene            1231..2700
                     /locus_tag="PK742_09335"
     CDS             1231..2700
                     /locus_tag="PK742_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76831.1"
                     /translation="MRTHVLEIWKALRTSAVVVMLPLSTWAQGGGAVWEYGPVMNQTR
                     IYAYTAALDDGRQIVFGGRESGFISCSYADVFNYESNSFTAFEMNYPRDAAAVVKMND
                     GRFFLAGGGYDYGIAPGYQDAEVFDPADNTFSYVGELFIPRMQCAGAQLADGRVLIAG
                     GWYNPDGAAVAELFTPSMSLFSMTGSMNTPRAAPVVLPCNDGGALVFGGWPTYGGDLY
                     TSVEYYDAVNNTFSEFSSELIPEDPGWLVHSSTESRPIQEKVMFDGRYLFKAYRNTPV
                     FEWALIAFDPDTKTFERISLDAGLTDGPPEGFYDLVLDAGGHMAYLLGYTYETTPYPV
                     CLVSVDLHTGHVYMPETSFTLGAGEWIMGTMSWNRVTEKILFTGISWSGADYFNGSNL
                     TYIIEPDYRYTVDMETIAEWNLSVYPNPAHDQVQIELPPEGNWMLLVTDMFGNRVWQQ
                     SGNGKEILVMPTGLWPAGMYLLTAEHNGRLQTMKIQVVH"
     gene            2765..3199
                     /gene="dut"
                     /locus_tag="PK742_09340"
     CDS             2765..3199
                     /gene="dut"
                     /locus_tag="PK742_09340"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQU76832.1"
                     /translation="MLRVPVINRSSFPLPAYATEHAAGLDLRAELKEAVILGSLERQL
                     IPTGIYIALPEGYEAQVRPRSGLAIKHGITMLNSPGTIDADYRGEIKVIVVNLSKEPF
                     TIQPGERIAQLVVAQYARISWQETEDLDETERGAGGFGSTGK"
     gene            3265..4269
                     /locus_tag="PK742_09345"
     CDS             3265..4269
                     /locus_tag="PK742_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQU76833.1"
                     /translation="MKIIIPMAGMGKRMRPHTLTVPKPLIAVAGKPIVQRLVEDIAAT
                     VSEPIEEIAFVVGHFGKQVEDGLIAIAESMGAKGKICYQDEPLGTAHAILCAGDTVSG
                     NIIIAFADTLFKTDFRIDPEKDGVIWVNKVDAWQNFGVVELDGDGHITNFVEKPQEFV
                     SDLAIIGVYYIKDGDTLRKEMQYLLDNDIRDKGEYQLTNALEAMRQKGAKFVPGAVDE
                     WLDCGNKDATVYTNQRVLELKKGSEQLIAGNLVSRNSVVIEPCYIGPDVVLDNAVVGP
                     HVSLGKGTVVKNAVVSNSIIQESVHIHNRIITNSMVGSHVVLEGHPDDLSLSDYSTSK
                     "
     gene            4270..>5405
                     /locus_tag="PK742_09350"
     CDS             4270..>5405
                     /locus_tag="PK742_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU76834.1"
                     /translation="MKQLIVGLLVLSGMWSACSAPQKTAGGVFAPVSTDGEYGSSQHS
                     PKADSLAMTRVFIDACRARSLGDDEGAVELFREVLQIDPLNDAALFELGKIYFEYGKL
                     DDARELLRLAAEQDPANTYYLETYGSALLYLAQYREAIEIYQQLLHIRPDDMDAAFQL
                     AFSFERAGSIEESLSFLRQMEQQFGPDESILMEEYRLFLRNGMLQEGIDVLLRLIENN
                     PDEPVYVSMLADVYELADQSELAEATFDKLLEIDPDNADLQFKKASYQIRSGDREAYW
                     NTLRKVFSNNRASIDKKVFFLVPYVDSVDKKNFTDKDSILEMARLMTIAHPTEAKAWA
                     MQGDLLYYAGQKTTARESFLKSTDIRADVYDVWVKMFYIDAEEER"
BASE COUNT         1403 a         1289 c         1402 g         1311 t
ORIGIN      
        1 ctacatgagg gttctgcatg accggcgaca gccatagtgt attgaagccc atattcgtga
       61 aatacccgtc ctgaagcttc tgctgtacac cagccagatc gccgcccata taattggctt
      121 tgggatcgac ccgcgggtca tcgaccggag cgtcattcgt tttgtcgccg ttgtagaaac
      181 gatccaccag caggaagtac acgatcgctt tgtggaagtc gtggcggttg ataagcgcag
      241 cgtcctccac aactttgcca aaggaaacag gaatcatcat atcgtttccc tcattgacct
      301 cattgtatcc aaaaacccgt atgctccctt caggttgccc tgccatttct tccggcatta
      361 caaatgaaag gctggtgcct tcactgaaag ggatcaggtg gttgttaatc aacacaaata
      421 caggatccag tgcgtttctg ttttcagatc cgataacgat cttattgtct tccgttttat
      481 cgaggtagaa atatggtctg ccggcaaaac tggtatctcc caccaccagc aggctgttga
      541 tgcccccgat accgttgggt acctgaacgg ggttattcgg atccagcatc caggaggtgt
      601 cagctacgat ctggtattga tagcgacctg gattgagcca caatcgtgtt tgccattgtc
      661 cgttgtggta ctccaatggc gttgcatcag gattccagct gttcatgtcg cctgccaggc
      721 taacatccag ataggcaact cctcccgggt tgaaaataaa cgacacttct tcctttttac
      781 ttctgcgcaa caccaggctc cactcctgtt caccggtgtg gaaagtgagt acactcaaag
      841 ggggtatgtc ctgccccgga acaatggtta cttgttgctt cagcgggtcc aaacgtgcct
      901 gcattctggg cggtgcttct actccggtca ccatggttgg tttttcgaaa taatctgcca
      961 gatagatcac ggtagtatct gtttccaggt tgacgggcat ccagtcgcca aaagtgttga
     1021 ccacctgcgc ctgtaactga acagaaaaca atgaagtggt aagcaatagc cagacagatc
     1081 tcattcggtg catttgaggc tgaaagttac aatgctctga tgatacgcat cagcggtgat
     1141 gcattgacag aaatctctcg agcatagtga tagatggcag ctcctgctgt tgtatcgtga
     1201 aatacattgc tgttaattaa acagatggtt atgagaacac atgtgctcga aatttggaag
     1261 gccctgcgca caagtgcagt ggtggtaatg cttccgctga gtacctgggc tcagggtggc
     1321 ggggcagtct gggaatacgg acccgttatg aaccagacca ggatctatgc ctatacagct
     1381 gccctggatg atggcaggca gatcgttttc ggaggcagag aatccggatt tatttcctgc
     1441 agctatgcgg atgtcttcaa ctatgaatcc aacagtttca cagcctttga aatgaactat
     1501 ccgagagatg ctgctgcggt tgtcaaaatg aatgacggcc ggttctttct ggctggcggc
     1561 ggctatgact atggcatagc acccggctat caggacgcgg aagtttttga cccggcagat
     1621 aacacgtttt cttatgtggg agagctattc attccccgca tgcaatgtgc cggtgcgcag
     1681 ctggctgatg gtcgggtact tattgcaggt ggctggtaca atcctgacgg agctgctgta
     1741 gcagaactat tcacaccttc tatgagcctt tttagtatga ccggctccat gaatacaccc
     1801 agggctgccc ctgtggtact tccatgcaat gacggtggcg cactggtttt tgggggttgg
     1861 cctacctatg gcggagatct gtacacctct gtagaatact atgatgcagt gaacaacacc
     1921 ttttctgaat tcagcagtga attgatcccg gaagacccgg gctggttggt acacagctct
     1981 acggagagcc gccccattca ggagaaggtc atgtttgatg gccgctacct ctttaaagcc
     2041 tatcgcaata caccggtatt tgaatgggcc ctgatcgcat tcgatccgga tacaaagacc
     2101 tttgagcgaa tttcactgga tgccggtttg accgacggac caccagaggg attttatgac
     2161 ctggttctgg atgccggagg acacatggcc tacttgctgg gatataccta tgaaacaact
     2221 ccctaccccg tttgtcttgt cagcgttgac ctgcataccg ggcatgtata tatgccggaa
     2281 acatccttta ccctgggagc cggcgaatgg atcatgggta cgatgtcctg gaacagggta
     2341 accgaaaaga tcctttttac aggtatttcc tggtcaggcg ccgactactt caatggcagt
     2401 aacctgacct atattataga acccgattac cgatacacag ttgatatgga aaccatagcg
     2461 gaatggaatc tgtctgttta ccctaatcct gcccatgatc aggtgcagat agaactgcca
     2521 cccgaaggca actggatgtt gctggtcact gacatgtttg gcaacagggt ctggcaacag
     2581 tcaggaaacg gcaaagagat cttggttatg cctaccggtc tctggcctgc aggtatgtat
     2641 ctgctgacgg cagaacacaa tggcaggctg caaacaatga aaatacaggt ggttcattaa
     2701 ttgcaacaat tcatcagatc aagaccggag ccggacagct ccggtctttc tgtatttttg
     2761 acagatgcta agagtgccag tcattaaccg gtcttctttc ccgttgcccg catatgccac
     2821 cgaacatgca gcaggtcttg atctgcgtgc tgaactgaaa gaagcagtca tcttaggttc
     2881 actggaacgt cagctcatac ctaccggaat atacatcgct ttacctgagg gttatgaagc
     2941 acaggtgcgt ccccgcagtg gtcttgccat caaacatggt atcaccatgc tgaacagccc
     3001 cggaaccatt gatgcagatt accgtggcga gatcaaggtc atcgtggtga acctcagcaa
     3061 agagcccttc accatccaac cgggagagcg cattgcacaa ctggttgtag ctcagtatgc
     3121 ccgcatatcc tggcaggaaa ccgaagatct ggatgaaacg gaacgtggtg caggaggatt
     3181 cggcagcacc ggaaagtgat cgaacagtcg cacagcaaca agccgaaaaa tgcacttctg
     3241 tatctttgca gcacatttga cattatgaag atcatcatcc caatggccgg catgggcaaa
     3301 cgcatgcggc cgcatacgct gaccgttccc aaacccctga tcgctgtggc aggtaaaccc
     3361 attgtgcaga ggctggtgga agatattgct gccaccgtga gcgaacccat tgaagagatc
     3421 gcttttgtgg ttggtcattt tggtaaacag gtggaagacg gactgatcgc cattgccgaa
     3481 agtatgggtg ccaaaggaaa gatctgctac caggatgaac cactgggtac agcacatgcc
     3541 atcctctgtg ccggcgatac cgtaagtggc aatatcatca tcgcttttgc tgatacgttg
     3601 ttcaagacag attttcgcat agatccggaa aaggacggag tgatctgggt caataaagtg
     3661 gatgcctggc aaaacttcgg agtggtagaa ctggatggag acgggcacat caccaacttt
     3721 gtggaaaaac cgcaggaatt tgtgtctgac ctggccatca tcggtgtcta ttacatcaag
     3781 gatggagata cccttagaaa agaaatgcag tacctgctcg ataatgatat cagagataag
     3841 ggtgagtacc agctcaccaa tgcactggag gccatgcgac aaaaaggagc gaagttcgtt
     3901 ccaggcgcag tagatgaatg gctggactgt ggcaataagg acgcaacggt ctatacgaat
     3961 cagcgggtgc tggaactcaa gaaagggtcc gaacagctca ttgccggcaa cctcgtcagc
     4021 cgcaacagcg tggtcattga accctgttat atcgggcctg atgtagtgct tgacaatgca
     4081 gtggtaggtc cgcatgtatc actaggtaaa ggaacggttg ttaaaaatgc tgtggtatcc
     4141 aattccataa tacaggaatc cgttcacatt cacaaccgga tcatcaccaa cagcatggtg
     4201 ggcagccacg tggtactgga aggtcatccg gatgatctta gtctgagcga ttacagcacg
     4261 agtaagtaaa tgaagcaact catcgtcggt ttactggtcc tttcaggaat gtggtctgcc
     4321 tgcagcgctc cacagaagac tgcaggaggt gtctttgcac cggtaagtac cgatggagaa
     4381 tacggcagca gccagcacag ccccaaggcc gacagcctgg ccatgacaag ggttttcatt
     4441 gatgcctgca gggcacgctc tctgggcgac gatgaaggtg cagtagaatt gttcagggag
     4501 gtactgcaaa tagacccgct caatgatgca gccctgtttg aactgggaaa gatctatttc
     4561 gaatacggca aactggatga cgccagggaa ttgcttcgtc tggctgcaga gcaggatcct
     4621 gccaacacct actacctgga aacctatgga agtgcactgc tctatctggc acaataccgg
     4681 gaggccatag agatttacca gcaactgttg catatcagac cggatgatat ggacgccgcc
     4741 tttcagctcg ccttttcctt tgaacgtgca ggtagtattg aagagagttt gtctttcctc
     4801 cggcagatgg aacaacagtt tggtccggat gaaagtattc tgatggaaga ataccggttg
     4861 ttccttagaa atggcatgtt acaggaaggc atagatgtcc ttctgcgtct aatagaaaac
     4921 aatcctgatg agccggtata tgtcagcatg ctggcagatg tatatgaact ggccgatcag
     4981 tcagaactgg cagaagccac ctttgataaa ctgctggaaa tagatcctga caatgcagac
     5041 ctgcagttca agaaggccag ctatcagatc agatcaggcg atcgtgaagc atactggaac
     5101 acactgcgaa aggtattttc caacaacagg gcttccatag ataagaaagt gttctttctg
     5161 gtgccttacg tagattcagt ggacaagaaa aattttaccg acaaagattc catcctggag
     5221 atggccagac tgatgaccat cgcacatccc accgaagcca aggcatgggc catgcaggga
     5281 gatctgttgt actatgccgg acaaaagacc acagcccgtg aaagcttttt gaagagtaca
     5341 gatatacggg cagatgtcta tgatgtctgg gtcaaaatgt tttatataga tgctgaagaa
     5401 gaacg
//
LOCUS       DAOMAX010000180         5398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2517_length_5398_cov_3.686880, whole genome shotgun sequence.
ACCESSION   DAOMAX010000180 DAOMAX010000000
VERSION     DAOMAX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2517_length_5398_cov_3.686880"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PK742_09355"
     CDS             complement(<1..825)
                     /locus_tag="PK742_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQU76835.1"
                     /translation="MWKQNILETIGNTPMVQLHHLVKDIPATVLAKVETFNPGNSIKD
                     RMALKMIEDAEKSGALKPGGIIIEGTSGNTGMGLAIAAVIKGYKCIFTSTDKQSPEKF
                     DALRAYGAEVIVCPTDVDPEDPRSYYSVSSRLVSEVPNAWKPNQYDNLSNREAHYLTT
                     GPEIWEQTEGRITHLVAGVGTGGTITGTGKYLKEKNPAVQVLGIDTYGSVFKKFKETG
                     EFDKNEIYPYVTEGIGEDFLPQNVDFSVIDHFEKVTDKDAAVMTRRIVREEAIWVGN"
     gene            complement(1058..2491)
                     /locus_tag="PK742_09360"
     CDS             complement(1058..2491)
                     /locus_tag="PK742_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQU76836.1"
                     /translation="MKRIAVLIIMIMWAWSLLAQPQMAYQTMKAGEGDSLGLKVEGDQ
                     TFIRYRIGSGETVMMVCRKYSTSLASIVSANPGLNPDYVQAGQVIFVPRPYKVAGGTT
                     TASNNNSGSGRYTVQAGETLTGICNQFNVSMSAMLSANPGLNPDLLRVGQTLAIPGAT
                     GNLNPPVTSIKSNYDGPTKGHTISSGETLYSIARLYGISFTQLMAANPNVQPDKIYPG
                     QEISIPTGDEETTASVTKQESKKEEKVVKKEEVRKEEVKKEEPVVKKEEPAVKKEEPE
                     VKKEEPVVKKEEPVTKKEEPAVKKEEPVQAKKEEPTPKETEIAKKEEPVKESNEAQET
                     KQSMKPDAAGMEASKETSPVTAGRNDREKSFAQVFAEYSSQTGDAKTEKGVATWIEGG
                     DAFGADSERFYALHNNAPIGSVIKVRNMMNNRVIYAKVIGTLSKEEVNDKVLVKLTSG
                     AAEKLNVLDSRFVSEITYYQVGKDYLR"
     gene            2595..3965
                     /locus_tag="PK742_09365"
     CDS             2595..3965
                     /locus_tag="PK742_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQU76837.1"
                     /translation="MRKSKFVRGLYTIFIGFTLLWTLFPLLATDNAGSGPRVFYFEIH
                     QDIMPSAWRTVQSALREADEADADYILLSLDTYGGLLDAADSIRTRLLSSNIPVIVHV
                     TNNAASAGALIAMSCDSIYMSSYAKIGAASVVDQSGNVMPDKYQSYMRGMMRATAEAN
                     GRDPDMAESMVGAIKTIPGIIDSGKVLTFTTSEAIENGFCEGKAETWQDALQLAGIED
                     YTLLRYEPSAMDKTMGLLLSPVLRGLCITFIFLGIYFELQTPGIGFPLAIAILAALLY
                     FAPLFVEGLAANWEILLFVVGIVLLALEIFVIPGFGVAGVSGIGLIFTALVLSMVQND
                     GFNFDFSGTGSLVEAFGIVMGAIAAAVIVIVAFFGRFLSSPLFKKVALLTSENAVEGY
                     NTNMPELSELKGVEGVAVSDLRPAGKVEINGDWYDAQTEAEYVLKGEQVIVLEVKNSY
                     LVVRKR"
     gene            3962..4684
                     /gene="kdsB"
                     /locus_tag="PK742_09370"
     CDS             3962..4684
                     /gene="kdsB"
                     /locus_tag="PK742_09370"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQU76838.1"
                     /translation="MKVLLIIPARFASTRLPGKPLALIGGLPMVVRVARQLQDVSGDK
                     EVVIATDDERIVEAAAKHQIQAVMTGDHVSGTDRCAATASMLPLSADIVVNVQGDEPF
                     IQASTIDRLIRAFDDPSVNIATLCFPIDREEDFRSEHVVKVVRDRSGNALYFSRAMIP
                     FPRQASEQKAYKHIGIYAFRNEVLQQVASLPPTALERTESLEQLRWLEHGYLIRVVES
                     DADTMGIDTPEDLAAANLKISE"
     gene            complement(4674..>5398)
                     /locus_tag="PK742_09375"
     CDS             complement(4674..>5398)
                     /locus_tag="PK742_09375"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HQU76839.1"
                     /translation="PLLGYAFDGYPIYGAYAYGNADGSGGITRMESGWAKRDITQRHT
                     LADGTVLDMAEWGPDVNDSYPLGAYKEDFEFTGGGDLDVHNGRFAVTPEYPEGTYAYY
                     TTIDEDGNSAFPYVIGETYCGIVAADNFPMGPVLTSVVISEPTNVYDPSTAVVLLELP
                     EIMLFPNPATDQVQLQVLGVLHTDQQVLATDLLGNSTLLKVWPAGTTRLAMDVSGLAA
                     GYYQLTIGNNQQQTHRSLMIIR"
BASE COUNT         1181 a         1373 c         1347 g         1497 t
ORIGIN      
        1 gttgcccacc cagatcgctt cttctctgac gatgcgacgg gtcatgacag ctgcgtcttt
       61 atcagtcacc ttttcaaagt gatcgatcac agagaagtcg acattctgcg gcagaaaatc
      121 ttctccgatc ccttcggtca catagggata gatctcattc ttatcgaact cgcccgtctc
      181 cttgaatttc ttaaaaacag aaccgtaagt atcaatgccc agcacctgga ccgccggatt
      241 cttttctttc agatatttac ccgtaccggt aatggtacca ccagttccca cacctgctac
      301 aaggtgggtg atccttcctt cggtctgctc ccagatctcc gggccggttg tcaggtagtg
      361 tgcttcgcgg ttggaaagat tatcatactg gttgggtttc caggcattgg gtacttcact
      421 taccaggcgg gaagaaacgg aataatagga gcggggatct tcaggatcta catctgtggg
      481 gcatacgatg acctcagcgc cataagctct cagtgcatcg aatttctcag ggctttgttt
      541 gtcggtactg gtgaagatgc atttatagcc cttgatgaca gcagcgatgg ccagacccat
      601 gccggtgtta ccggaagtgc cttcaatgat gatgccacca ggtttcagcg caccactttt
      661 ctcggcatct tctatcatct ttaaagccat acggtctttg atagaattgc ccggattgaa
      721 ggtctctact tttgccagta ctgttgcagg aatgtctttt accaggtggt gaagctgcac
      781 cattggagtg ttaccaatgg tctcgaggat gttctgtttc cacatggtgc gaaggtagtg
      841 ggttttgggg agctgtttga agttggcagt cagcagtcag cagtcagcag tcagcagtca
      901 gcagtcagca gttaaaaaat cgggatacaa tttactacca ctttaaattt cacccttcac
      961 ctttccactt tcaactttcc ctttccccca aaagagaaac cccgcattgc tgcggggccc
     1021 tcttctcata cttgaacctg attatgaacc taaaacttta tctcagatag tctttgccaa
     1081 cctggtagta ggtgatctca ctaacgaatc tgctgtccag tacattcagt ttttcggcag
     1141 cacctgaggt gagttttacc agcactttgt cgttcacttc ttccttgctc agcgtgccga
     1201 tcaccttggc gtaaatgacc cggttgttca tcatgttacg caccttaatt acgctgccaa
     1261 tgggcgcatt attgtgtaag gcataaaatc tttcagagtc tgcaccgaat gcatcacctc
     1321 cttctatcca ggtagcaaca cctttttcgg tcttggcatc acctgtctga gaagagtatt
     1381 ctgcaaatac ctgggcaaat gatttctccc tgtcattcct gcctgccgtt accggactgg
     1441 tctctttgga agcttccata cctgcggcat ccggtttcat ggactgcttg gtttcctgtg
     1501 cttcgttgct ctctttgaca ggttcttctt tctttgctat ttcagtctct ttcggcgtgg
     1561 gttcttcttt cttagcctga acgggttctt ctttcttgac cgctggctct tcttttttag
     1621 taacaggctc ttccttcttc accaccggtt cttctttctt cacctccggt tcttccttct
     1681 tcactgcagg ctcttctttt ttaactaccg gctcttcttt cttcacttct tctttgcgaa
     1741 cctcttcttt cttcaccacc ttctcttcct tcttgctttc ctgtttggta acggaagctg
     1801 tggtctcttc atcgccggtc gggatgctta tttcctgacc agggtagatc ttgtcaggct
     1861 gcacgtttgg attggcggcc atcagctggg tgaaactgat gccatacaaa cgggcgatgc
     1921 tgtacagggt ctcgccggaa gatatagtat ggcctttggt cgggccatca taatttgatt
     1981 tgatggaggt gacaggtggg ttgagattgc ctgttgcacc cggaatggcc agggtctgtc
     2041 ccacacgcaa caggtccgga ttcagacccg ggttggcaga cagcatggca ctcatgctca
     2101 cattgaactg gttacagatg ccggtaagcg tttcgccagc ctgaaccgta tatctgccag
     2161 agccggagtt attgttactt gctgtggtgg tgccacccgc taccttataa ggtctgggaa
     2221 cgaaaatgac ctgacccgcc tgtacatagt ccggatttag tccggggttg gcagacacga
     2281 tgctggctaa agaggtgctg tacttgcggc acaccatcat gaccgtttcg ccggaaccaa
     2341 tgcggtatct gatgaaggtc tgatcaccct ccactttcag tcccagcgag tcaccctcgc
     2401 cggctttcat ggtttgatag gccatttgtg gctgggccag gaggctccat gcccacatga
     2461 tcatgataat aagtactgct atccttttca tgtttttttg ttgcagtaaa acgtgcatac
     2521 gtttaccttt agagcagaca tcagacaccg gcagtgcctg agctgcggtt ttgacaaaat
     2581 tacttgaagc ggctatgcgc aaatcaaagt tcgtgagggg tttatacacc atttttattg
     2641 gatttacatt attgtggaca ctttttcccc tgctggcaac agataatgcc ggttccggtc
     2701 cccgtgtatt ctatttcgag atacaccagg atatcatgcc ctctgcctgg cgcacggtac
     2761 aaagtgccct gcgggaagca gatgaagctg atgccgacta tatcctgctc agcctggata
     2821 cctatggagg attactggat gcggcagatt ccataagaac ccgcctgctt tcttccaata
     2881 ttcctgtcat cgtgcatgtg accaacaacg ctgccagtgc cggtgcgctc attgctatgt
     2941 catgtgacag tatttacatg agcagttatg ccaagatagg cgcagccagt gtagtggatc
     3001 agagtggtaa cgtcatgccc gataaatacc agagttatat gcggggcatg atgcgggcta
     3061 ccgccgaagc caacggacgt gatcccgata tggccgaaag catggtgggt gccatcaaga
     3121 ccattccggg aatcattgac tcaggcaaag tgctcacctt tactaccagc gaagccatcg
     3181 aaaacggatt ctgcgaaggc aaggcagaga cctggcagga tgcgttgcaa ctggcaggta
     3241 tagaagacta tacccttctg cggtatgaac ccagcgccat ggacaagacc atgggccttc
     3301 tgctgagtcc ggtgctgcgt ggtctgtgca tcacctttat tttcctgggc atctattttg
     3361 aattacagac gcctggtatc ggatttccac tggccatagc catcctggcg gcactgttgt
     3421 acttcgctcc gctttttgtg gaaggtcttg ctgccaactg ggagatcctg ctcttcgtgg
     3481 tggggatagt cctgctggca ctggagatat ttgtcatacc cggattcggt gtggccggtg
     3541 tatccggaat cgggctcatt ttcacggctc ttgttttgag catggtgcag aatgatgggt
     3601 tcaattttga cttcagcggt accggctctc tggtggaggc cttcgggatc gtgatgggtg
     3661 ccattgctgc agcggtcatt gtgatcgtgg cattcttcgg acgtttcctg agttcgccgc
     3721 tattcaagaa agttgcgctg cttaccagtg agaatgccgt ggaaggatat aacaccaata
     3781 tgcctgaact ttctgagctg aagggagtag aaggagtggc cgtatctgat ctgaggcctg
     3841 ccgggaaggt agagatcaac ggcgactggt atgatgctca aaccgaagct gagtatgtat
     3901 taaaaggaga acaggtgatc gtactggaag tcaagaattc atacctggtg gtgcggaagc
     3961 gatgaaggtc ctgctgatca tacctgcccg ttttgcttcc acccgtttgc cggggaagcc
     4021 gcttgctttg attggcgggt tgccgatggt ggttcgggtg gccaggcaac tgcaggatgt
     4081 aagtggagat aaggaagtgg tcattgccac cgacgatgaa cggatcgtgg aagcagctgc
     4141 caaacaccaa attcaggctg tcatgaccgg cgatcatgtg tccggtactg atcgctgtgc
     4201 tgccacagca tccatgttac ccctttcggc agatatcgtg gtcaatgtgc agggcgatga
     4261 accattcatc caggcttcca ccatcgaccg tttgatacga gcattcgatg atccttcggt
     4321 caatattgcc actctctgtt ttcccatcga ccgggaagaa gacttccgca gtgagcatgt
     4381 ggtcaaagta gtgcgtgacc gctcaggcaa tgctctgtat ttttcgcggg caatgatccc
     4441 ctttccccgt caggcatcag aacaaaaagc atataaacat atcggtatat atgcttttcg
     4501 aaatgaggtg ttacaacagg ttgcatcact tccccctact gcacttgaac gcacagaatc
     4561 tctggaacag ctgcggtggc tggaacacgg ctatctgatc agggtagtag aatctgatgc
     4621 tgataccatg ggcattgata ctccggaaga cctcgcagct gccaacctga agatcagcga
     4681 atgatcatca gtgacctgtg tgtctgttgc tggttgtttc ctatcgttag ctgataataa
     4741 cctgcagcca gtcctgatac atccattgcc agccttgtgg ttccggcagg ccacaccttc
     4801 agcaaggtgc tgttgccaag cagatctgtt gccagtacct gctggtctgt atgcaacaca
     4861 cccagtacct gcaattgcac ctggtctgtt gcagggtttg gaaacaacat tatttcagga
     4921 agctccaaaa gcacaacagc agttgacgga tcatatacat tagttggctc tgatatgacc
     4981 accgaggtca gcacgggacc catcggaaaa ttatcggccg caacgatacc gcaataggtc
     5041 tcaccgatca cataggggaa agctgaattt ccatcttcat ctatggtggt ataataggcg
     5101 taggtgcctt ccggatattc cggtgtcacc gcgaatcttc cgttgtgaac atccagatcg
     5161 ccgccaccgg taaattcaaa atcttctttg taggcaccca gtgggtagga gtcattgaca
     5221 tcgggtcccc actcagccat atctagcact gtaccgtcgg ccagggtatg tctttgggtg
     5281 atgtcccgct ttgcccagcc tgattccatc cgcgtgatac caccgctgcc atcggcgttg
     5341 ccataagcat aggcaccgta gattggataa ccatcaaagg cataacccag cagcggtg
//
LOCUS       DAOMAX010000181         5385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2529_length_5385_cov_4.084991, whole genome shotgun sequence.
ACCESSION   DAOMAX010000181 DAOMAX010000000
VERSION     DAOMAX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_5385_cov_4.084991"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..619
                     /locus_tag="PK742_09380"
     CDS             146..619
                     /locus_tag="PK742_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76840.1"
                     /translation="MKYPLSLFLMTLVIAGCTDKKEPFIRYNDVIVEQVYLADSTLRD
                     VFSFRDFETFPDRHPGYDASFEGIRKSLHDLEAMEEDSLRQAAMDLVNAYEGIVDDDY
                     ATIYQLMHDSIYTQSDSIQVDSLSAEMYAKWQIYSEKFASTQKHFARKNGISLDK"
     gene            complement(734..1072)
                     /locus_tag="PK742_09385"
     CDS             complement(734..1072)
                     /locus_tag="PK742_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76841.1"
                     /translation="MKTPYRQTGDSTLFWLDDYSEFCDVMMIAGYWMGVCAGGLNPGI
                     SESITLYDSCGLPFGRIRRLHGFSEKIDTLYAWQLELEQERYDDLKNKLKKKEVVTVR
                     FDDKLVAIGN"
     gene            1137..1454
                     /locus_tag="PK742_09390"
     CDS             1137..1454
                     /locus_tag="PK742_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76842.1"
                     /translation="MSFTGNEGTTISLQQGAAMTRKYCNSTTESRKAVFYGINRLNEI
                     LSQQGCVGIRIYLARDGADNMQLVLVGADANEQDMLSGVILDQGLGCPENCSADNPLN
                     SDQ"
     gene            1460..2125
                     /locus_tag="PK742_09395"
     CDS             1460..2125
                     /locus_tag="PK742_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76843.1"
                     /translation="MSQLYMNILYIMYVSYLVALAGGIHSRVFRFRAGRPLFIYLCIE
                     IASEITSYITVIIYHVNNQFVMHVDSIMDLLMFLSFYRMILKPGTVRNLLPFAAIVGL
                     LIGAADWWLLAGPYVQPSFARTSHLLLIAPLPLVFFQQLLQNPEVEDLRTYPLFWINT
                     AALFKYFGLLAVSSVGYLIHNAEVGSSFRVLWNINNLVNIVFNVLLGIGFYRWKTPVT
                     SYS"
     gene            2095..2880
                     /locus_tag="PK742_09400"
     CDS             2095..2880
                     /locus_tag="PK742_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU76844.1"
                     /translation="MENAGDIIFLIFVSTLLFLTLALLVVVFVVLYQKRFVQQKQIIQ
                     EQETIYRQRLLESVMETQEEERQRIARNVHDDVGTLLNVAKMQLGSIRSADEHQHEKL
                     GQLGQHLQESIDQLRAIARSLSPPVLDKLGLFKALEDLAGRTKVTGAVKVNFSGEEGN
                     AVNRELANHLFRMIQEVFTNTLKYASPTSIQLQYESDDEGIGILFRHDGNGLIQEEVK
                     NISDSGKAFGLDSVLSRGLVSNTRVMFGKDEEGYFVEFQHPYI"
     gene            2877..3521
                     /locus_tag="PK742_09405"
     CDS             2877..3521
                     /locus_tag="PK742_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU76845.1"
                     /translation="MIRVGIVDDQPLFVQGLCRLLEDQQELHIECTANSGLELTTRLD
                     QHHIDILLMDLEMPEEDGFQLIKRLQQDHPDVLVIVLTMHNDPAIIIEMVQLGARGFL
                     TKDAAVREIIDAVFSVRDTGYFFNDHMSTRVLCEAVKKGNVSASFSKPELSEREQEVL
                     RLICAEKTNKEIADHLILSVRTVDGHRERILAKIGARNTAGIVLYAIRNGLVEL"
     gene            complement(3522..4481)
                     /locus_tag="PK742_09410"
     CDS             complement(3522..4481)
                     /locus_tag="PK742_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76846.1"
                     /translation="MNRNIVVAAIMILAVSASLQSCSSEAGASPDTNTASEPVAVASY
                     TPSAEWNAYWYQGVAEVNSYKVSQGRYTMSHEGTCVNIFVTEDFSKEKLVKLNDAGRA
                     GADRLPILKLNQSLKFNTGIYPYSVMMSAFQPVDRNNYPHAVKVTNTVQEWCGMAYMQ
                     AKMSGTQVGIEQFSYFEGEQDQSYAFDGTFMEDELWTTIRLFPDELPQGEMLVVPGTT
                     FLRFSHKPVRPYTAKISVSKADGVRVLKIEYPELSRDMEISFEDVFPYKILGWEEDYL
                     GFNGEKLHTEAKLDKTIMIDYWNKHNNDERSLRKDLGLPEDWQ"
     gene            complement(4534..>5385)
                     /locus_tag="PK742_09415"
     CDS             complement(4534..>5385)
                     /locus_tag="PK742_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HQU76847.1"
                     /translation="CNGKYVYADPFKGTMMAVSEAARNIVCSGGEPAAITNCLNFGNP
                     YNPEVYWQFVQAIKGMGEACKAFDTPVTGGNVSFYNQSGTEAVYPTPTIGMVGVVEDM
                     SRSTGLGFTKEDDVILLLGNTTADMHSSEYIKAIHGIAVSAAPHFELQEEVNLQHAVR
                     ELIRKKLVHSAHDISEGGLFITLLEKAMAGGMGFSVETDSSIRKDAFLFGEGQSRVVL
                     TCKASSVEDIEEICIIHGVRINSLGSVGGDRLEIDGADFGSLDDYRMMYDNAIGNLMQ
                     SESELVS"
BASE COUNT         1378 a         1332 c         1285 g         1390 t
ORIGIN      
        1 ctgtggtcat ctaccagcac aatatcctct cttccctgcc tgttcatatg ccccgccaga
       61 caactgccaa tgaaccccgc tgcaccggtt ataatgatca ttttgctacc tttgattagc
      121 tgtaaagttc tataatccgg agcgaatgaa atatcctctt agtttgtttc tcatgacact
      181 ggtgattgcc ggttgtaccg acaagaaaga accctttata agatataatg atgtgattgt
      241 ggaacaggtg tacctggccg acagcaccct tcgggatgtt ttttcttttc gtgattttga
      301 aacctttcca gaccggcacc ccggatatga tgcatctttt gaaggtatca gaaaatcact
      361 ccatgatctg gaggccatgg aagaagacag cctccgtcag gctgcaatgg atctggtcaa
      421 tgcgtatgaa ggaattgtgg acgatgacta tgccaccata taccagctca tgcacgacag
      481 tatctatacc caaagtgaca gtattcaggt ggacagcctg tcggcagaaa tgtatgccaa
      541 atggcagatc tacagcgaga agtttgccag cacccagaag cacttcgcaa gaaagaacgg
      601 tatctccctg gataaataat aagggaagcg ccgatcaagc gctcccctcg ttctgattgg
      661 ccatctatgg catccgtggt gaaccacagc cgggagcctg gccatactcc cattcaacgg
      721 gtcgacttgc tgatcaattt cctatagcca caagtttgtc atcgaaacgc acagtgacca
      781 cttctttttt cttcaacttg ttcttcaggt cgtcatatcg ttcctgctcc aattccagct
      841 gccaggcata cagcgtatca atcttctctg aaaacccatg caggcggcgg atcctgccaa
      901 aaggcaatcc gcatgaatca tacagtgtga tggattcaga gatcccgggg ttgagccccc
      961 cggcacatac acccatccaa tatccggcaa tcatcatgac atcacaaaat tcactgtaat
     1021 catccaacca gaacagcgta gaatcacctg tttggcgata gggtgttttc accctaaata
     1081 aagttgtcct gcataaggtt tggtcaaatc tgctatactt gtaaaaaaca cacactatgt
     1141 catttaccgg aaatgaggga actacgatct cactgcaaca aggagcggcc atgacaagga
     1201 aatactgcaa cagtaccact gagtcaagaa aggctgtatt ctatgggatc aacagattga
     1261 acgagatcct gtcacaacaa ggatgtgttg ggatacgcat ctatctggct cgtgacggcg
     1321 cagataatat gcaactggtt ctggttggag ctgatgcaaa cgaacaggac atgctgagcg
     1381 gcgtcattct ggatcagggt ttgggttgtc cggaaaactg cagcgcagac aaccctctaa
     1441 acagtgatca atagcgtgga tgagtcagct ttacatgaat atcctgtata tcatgtatgt
     1501 ctcctacctg gttgccttgg cggggggcat tcacagcagg gtattcagat tcagggccgg
     1561 cagacctttg ttcatctatc tctgcattga gatcgcctcg gagatcacct cctatataac
     1621 agttatcata tatcatgtca acaaccagtt tgtgatgcat gtggacagca tcatggacct
     1681 gctgatgttc ctttcttttt accggatgat cctgaagccg ggaaccgttc gaaatctact
     1741 gccgtttgct gctattgtcg ggttactgat cggtgccgcc gactggtggt tgctggccgg
     1801 accctatgtt cagccaagtt ttgccagaac ctcacacctg ttgctcattg cgcctttacc
     1861 tttggttttt tttcagcaac tgcttcagaa tcctgaggtg gaagatctgc gcacgtatcc
     1921 gctattctgg atcaataccg cagcgttatt taaatacttc ggactactgg cggtgagtag
     1981 cgtcgggtac ctcatacaca acgctgaagt agggtccagc ttccgggtgc tctggaacat
     2041 caataatctt gttaatattg tttttaatgt tttattaggt ataggattct atagatggaa
     2101 aacgccggtg acatcatatt cctgatcttt gtatctaccc tgctgttcct gacactggca
     2161 ctgctggtag tggtctttgt ggtgctgtat caaaaacgct ttgtccagca aaaacagatc
     2221 attcaggaac aggagaccat ttacagacag cgacttcttg aatctgtgat ggagacccag
     2281 gaggaagaaa gacagcgcat tgcaaggaat gtacacgatg atgtaggtac gctgcttaac
     2341 gtggcaaaga tgcaacttgg aagcatccgc tctgccgatg agcatcagca cgaaaaactc
     2401 ggacaactcg gtcagcacct gcaggaaagc atcgatcagc tgagggccat tgcaaggagt
     2461 ctgtctccgc ctgtactgga caaactgggt ttgttcaaag cactggaaga tctggccggc
     2521 agaaccaagg ttaccggtgc agtgaaagtg aacttttcag gtgaagaagg caatgcggta
     2581 aatcgcgaac tggcaaacca cttattcaga atgatacaag aggtgttcac caataccttg
     2641 aaatatgcat cccccacatc cattcaacta cagtatgaat cagatgatga gggtatcggg
     2701 atcctgtttc ggcatgacgg aaacggattg atccaggaag aagtcaaaaa cataagtgac
     2761 agcggaaaag cattcggcct ggacagtgta ttaagccgcg ggcttgtcag caataccagg
     2821 gtcatgtttg gaaaagacga ggaaggatat tttgtagaat ttcaacatcc gtacatatga
     2881 tcagggtagg tattgtggat gatcagcccc tgtttgtaca gggtttatgc aggcttctgg
     2941 aagaccagca ggagttacac attgagtgta ccgccaatag tggcctggaa ctgaccaccc
     3001 gactggatca gcatcacatt gatatcctgc tgatggatct ggagatgcct gaagaagatg
     3061 gattccagct gatcaaaaga ctccagcagg accatcccga tgtgctggtc attgtactga
     3121 ccatgcacaa tgaccctgcc atcatcattg aaatggttca gctgggtgcc aggggctttc
     3181 ttaccaagga tgctgccgta cgggagatca ttgatgctgt tttttcagtg agagacacag
     3241 gctatttttt caatgaccac atgtcaacca gggtgttgtg cgaggcggtc aaaaagggga
     3301 atgtaagtgc cagcttcagt aagcctgaac tgagtgaacg ggaacaggaa gtgcttcgtt
     3361 tgatctgtgc cgaaaaaaca aacaaggaga tcgcagacca cctgattctg agcgtcagaa
     3421 ccgtagacgg tcacagagag cgcatcctgg ccaagatagg cgccagaaat acagcgggca
     3481 ttgtgttata tgccatcagg aatggcctgg tggaactctg atcactgcca gtcttccggc
     3541 aaacccaagt ccttgcgaag ggaacgttcg tcgttgttgt gcttgttcca gtagtcgatc
     3601 atgatggtct tgtccagctt ggcttctgta tgcagctttt caccattgaa tccgagatag
     3661 tcttcttccc atcccaggat cttatacgga aatacatctt caaaactgat ctccatatcc
     3721 cggcttagtt ccggatattc gatcttcaat acccggactc cgtcggcttt gctgaccgag
     3781 atcttcgcag tatatggcct gacaggttta tggctgaaac gcaggaaggt cgttcccggc
     3841 actaccagca tttcgccctg aggcagctca tccggaaaca atctgatggt ggtccataat
     3901 tcatcctcca tgaaagtacc atcgaaggca taactctgat cctgttcacc ttcaaagtag
     3961 gaaaactgtt caatgcccac ttgtgtacca ctcatttttg cttgcatgta tgccatcccg
     4021 caccattcct gcacagtgtt ggtcaccttg accgcatgcg gataattatt tctgtcaacc
     4081 ggctggaagg cagacatcat cacggaatac ggatagatac ccgtgttgaa cttcaagctc
     4141 tggttgagct tcaggatcgg gagcctgtcg gcaccggcac gacctgcatc gttcagctta
     4201 accagttttt ctttggaaaa gtcttccgta acaaaaatat tgacacaggt gccctcatgg
     4261 gacatggtgt acctgccctg gctgacctta tagctgttca cttcggccac accctgatac
     4321 cagtaagcat tccactctgc cgatggtgta taggatgcta ctgcaacagg ctcagatgct
     4381 gtattggtgt caggagatgc tcctgcttct gagctgcagg attgcaggga agcagaaact
     4441 gccaggatca tgatcgcggc aactacaata tttctgttca tagcagacgt gttttgtatg
     4501 tcaacaactg acgggcaaat tcgttccgtt cggtcaggaa acgagctctg attcagactg
     4561 catcagattt ccgatcgcat tgtcatacat catccggtaa tcatccaggg aaccgaaatc
     4621 tgctccgtct atctccagcc tgtcgccacc cacagaaccc aggctgttga tcctgacccc
     4681 atggatgata cagatctctt caatatcctc cacagaagaa gctttgcagg tgagcactac
     4741 ccggctttgt ccttccccga acaggaaagc atccttgcgg atggaactgt cagtttccac
     4801 agagaatccc ataccaccgg ccatggcctt ttcgaggagg gtgatgaaca acccgccttc
     4861 agagatgtcg tgtgcactgt gcaccagttt ttttctgata agctccctta ctgcatgctg
     4921 caggttgacc tcttcctgca gttcaaaatg cggagcggca ctaacagcga tgccgtggat
     4981 ggctttgatg tattctgatg aatgcatgtc ggctgtggta tttcccagca acaggatcac
     5041 atcatcctct ttggtgaatc caagtccggt gctgcggctc atatcttcta ccacgcccac
     5101 catgccgatg gtcggggttg gatagaccgc ttccgttccg ctctggttat agaaactcac
     5161 attacctccg gtaacaggcg tatcaaaagc cttacaggcc tcgcccatac ctttaatggc
     5221 ctgcacgaac tgccagtaca cttccgggtt atacgggttt ccaaagttca ggcagttggt
     5281 gatggctgca ggttctccgc cggagcacac gatgttcctg gcagcttcac tcaccgccat
     5341 catggtgcct ttgaaaggat cggcatagac atattttccg ttaca
//
LOCUS       DAOMAX010000182         5373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2541_length_5373_cov_4.502068, whole genome shotgun sequence.
ACCESSION   DAOMAX010000182 DAOMAX010000000
VERSION     DAOMAX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_5373_cov_4.502068"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1252)
                     /locus_tag="PK742_09420"
     CDS             complement(53..1252)
                     /locus_tag="PK742_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQU76848.1"
                     /translation="MARIQKHIATICAEAGKHTEAPNVHTEPIYATSAYLFEDADDAM
                     RKLSDKRLGYVYSRWANPTVDQAAEKIAALEGFEAGTLTFAQLFSSGMAAIASVMLAT
                     CKAGDKILTQTQLYGTTDELMQSLLPEYAIETRSTDMTDLSKVAEILHADHRIKLIYL
                     ETPSNPLIQLVDIRAIVQLAAKYHIKVAVDNTFCTPLVQQPLAMGADFSIHSTTKFLN
                     GHGTALGGVVVGRSRRFMQEHIWRQVKLLGGNSNAFDAWLLLQGMKTLSLRMERHVQN
                     ATKVAAYLEQHPNVKQVYYAGSKNHPQYKLAKKQMKNGGAMLSFELKGGLNAGKRLMN
                     RVQICKLVTSLGTLDTLVQHPASMTHVNVPAERRLASGITDGLVRMSVGVEDVRDIIY
                     DLEQGLK"
     gene            1324..2613
                     /locus_tag="PK742_09425"
     CDS             1324..2613
                     /locus_tag="PK742_09425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQU76849.1"
                     /translation="MAYHYNDDRLPTIVVIGGGFAGLELVKKLNNKPFRVILIDKRNY
                     HTFQPLLYQVASAGLSADSIGFPFRKKIGPYPNIAFRMAEVQEVDPAAKKVITNVGTF
                     LYDHLVIATGTTTNYFGNKNLASWGLPLKSIGEALDLRSEILQELEKAIVTETVEERE
                     STLQFVIVGGGPTGVELAGALAEIQRNVLPTDYTEIDRKQMKVVLIEAADRLLATMSE
                     KSSKAAKKYLEKLGVEVRLDTRVQDYDGASGVLQLADGGTLRTRNIFWTAGVKADPLP
                     GIPAENIGRGGRYLCDTMNRITGLENVYALGDTSLIQGDAAYPNGHPQVAQVAIQQAH
                     RLASNFLRWETGKKGNAFSYKDKGSMATIGRHKAVVDLPGFSFSGYFAWYIWMFIHLM
                     ALVGFRNRFMVFMNWMWNYFTYDKALRLIVRPYKRPQ"
     gene            2717..3109
                     /locus_tag="PK742_09430"
     CDS             2717..3109
                     /locus_tag="PK742_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQU76850.1"
                     /translation="MNIIMYVYPMQDLKQRTKKFAIDCIGLCRKIQYSPESKVITNQL
                     LRSSTAVAANYRAACRLKSRADFIAKITTVEEEADESQFWLELIQDASIFIDKEVDRL
                     QDEAGQLTAIFASSAKTAKANNTLRTKK"
     gene            3219..3932
                     /locus_tag="PK742_09435"
     CDS             3219..3932
                     /locus_tag="PK742_09435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQU76851.1"
                     /translation="MLHINPEQLFIFDIETVPGVKDFKELSPELQDLFDDKVGRHKEE
                     GVSAADYYNAKAGIFAEFGKVIVISCGYMKKKGNGYDLKVKTFSNHNEKQLLEEFAQT
                     LINISKSNYFLCGHNIREFDVPWLCRRMLINGIPLPPCLDLYGKKPWEVNHVDTLDLW
                     KFGDYKHYTSLHLLSTILGIPTPKDDIDGSMVGEVYWKQDDLPRIAKYCSKDVVAVAN
                     VILKLKGEKILGEEHIELS"
     gene            complement(3955..4791)
                     /locus_tag="PK742_09440"
     CDS             complement(3955..4791)
                     /locus_tag="PK742_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76852.1"
                     /translation="MSDLHMHWKCTLLFLFSGLSSIGIYGQSAIPVPGGSHTDSWYIY
                     WGWNREVYTRSDIHFQGADYDFTLQDVRAIDRQTKFSVNPYLNPVKMTIPQYNFRLGY
                     YLNDHWNVSLGIDHMKYVMVQRQVVTMNGWIGGDHTYTGTYDERSQRLNKDFLVFEHT
                     DGLNDLNLELRYSSVLGEWPQVTLRHTEGGGVGVLIPRTNTTLLGMERYDEFHLAGWA
                     LQVMAGLQLTFFERFLIQSEVKTGLIDMPDIRTTSSVLDKASQHFLFLQGNLVFGVAF
                     GR"
     gene            complement(4830..5219)
                     /locus_tag="PK742_09445"
     CDS             complement(4830..5219)
                     /locus_tag="PK742_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76853.1"
                     /translation="MLKKVIFPVLLAMIWISIHEFVRNQVVLTQHWTDHYASMGLVFP
                     ADPVNGAVWGVWALLLAGFIRIITPKFSFMETVFLSWLAGFIMMWVVIGNMGVLPVQI
                     LPVAVPWSLFEVFGAVWITRKMAIVSE"
BASE COUNT         1429 a         1341 c         1297 g         1306 t
ORIGIN      
        1 tattccgaat tccgtattcc ccatttcgca ttccgcattt cgaattccgc cttcacttca
       61 acccctgttc caggtcgtag atgatgtctc tcacatcttc cacgcccaca ctcatgcgca
      121 ccagtccgtc ggtgatgccg ctggccagtc tgcgctctgc cggcacattc acatgcgtca
      181 tactggcagg atgctgcacc agcgtgtcaa gtgtgccaag gctggttacc agtttgcata
      241 tctgcacgcg gttcatgagt cttttgccgg cattcaatcc gcctttcaat tcaaaactca
      301 gcatagcccc gccattcttc atctgtttct tagccagttt gtattgagga tgattctttg
      361 atccggcata atagacctgt ttcacgttcg gatgttgttc cagataggct gccactttgg
      421 ttgcattctg cacatgtctt tccatgcgca gtgacagcgt cttcataccc tgcagcagca
      481 gccaggcatc aaaggcatta ctgttgcctc ccagcagctt tacctgtcgc cagatatgtt
      541 cctgcatgaa cctgcggctg cgccctacca ccacaccacc taaagctgtt ccatgaccat
      601 tcaggaattt ggtggtgctg tggatggaga aatcagcacc catagccagc ggttgctgca
      661 ccaggggtgt acagaaggta ttatctacag ccactttgat atgatatttc gccgcaagct
      721 gaacgatggc acgaatatct accagctgaa taagcgggtt ggaaggtgtt tcgaggtaga
      781 tcagcttgat gcgatgatca gcatgcagga tctctgccac ctttgaaaga tcggtcatat
      841 ccgtacttcg tgtttcgatg gcatattccg gaagcagtga ctgcatcagt tcatcagtag
      901 taccgtagag ctgggtttgg gtcaggatct tgtcgcccgc cttacacgtg gccagcatta
      961 ccgaggcgat ggctgccatt ccgctggaga acagctgggc aaaggtcagg gtgcctgcct
     1021 caaaaccttc cagcgctgcg atcttttctg ctgcctggtc aacggtagga ttggcccatc
     1081 tggaataaac atatccaagc cgtttgtcag acaacttccg catggcatca tcagcatctt
     1141 caaagagata ggctgaggtg gcataaatgg gttccgtatg tacattgggt gcctctgtgt
     1201 gtttgcctgc ttctgcacag atggtagcaa tatgtttctg tattctggcc atgtgagatt
     1261 attattggaa tctttgcatc aaagtaaacc attccgctcc ttatttcctt taatatgtaa
     1321 ggcatggcat accactacaa cgacgatcgt ttacccacca tagtggtgat aggcggaggt
     1381 ttcgcaggac tggagctcgt caagaagctc aacaacaaac cctttcgtgt catcctgatc
     1441 gacaagcgga attaccacac cttccagcca ttgttgtatc aggtggcctc agcgggtctt
     1501 agcgctgaca gtataggatt ccccttccgg aaaaagatcg gcccgtatcc caacatcgcc
     1561 ttccggatgg ccgaggttca ggaggttgac ccggcagcca aaaaggtcat caccaatgtg
     1621 ggcacctttt tatacgatca tctggtcatt gccaccggaa ccactaccaa ttactttggc
     1681 aacaagaatc tcgcttcctg gggtcttccc ttgaagagca taggcgaagc actcgatctg
     1741 cgcagtgaga tcctgcagga actggaaaag gccatcgtta ccgaaacggt ggaagagcgg
     1801 gaaagcaccc tgcaatttgt cattgtgggt ggaggaccta cgggtgtgga actggcaggt
     1861 gccctggcgg agatacaacg caatgtgctg ccaacggatt ataccgagat agacaggaaa
     1921 cagatgaagg tagtactcat tgaagcggcc gacaggttat tggcaaccat gagtgaaaag
     1981 tcatcgaagg cagctaaaaa atacctggaa aaactgggtg tggaagtgcg cctggatacc
     2041 cgggtacagg attacgatgg ggcatccggt gtgctgcagc tggccgatgg cggtaccctg
     2101 cgcacgagga acatcttctg gacagccggt gtgaaggccg atccgctacc cggcattcct
     2161 gccgaaaata tcggtcgcgg ggggaggtac ctctgtgaca ccatgaacag gatcacagga
     2221 ctggaaaacg tatatgcgct gggcgatact tctttgatac agggagatgc agcgtatccg
     2281 aacggtcacc cgcaggtggc tcaggtggcg atccagcagg cacaccgact ggcctccaat
     2341 ttcctgcgat gggaaacagg taagaaagga aacgctttca gctacaaaga caaaggaagc
     2401 atggctacca tcggacggca taaggctgtg gtggacctgc ccgggttctc tttctcaggc
     2461 tacttcgcct ggtatatctg gatgttcatc cacctgatgg cactggtggg cttccgcaac
     2521 cgcttcatgg tcttcatgaa ctggatgtgg aactacttca cctacgacaa agcgctgagg
     2581 ctgatcgttc gaccctacaa acgaccacag taaaaattcg gaattcggaa ggcggagtgc
     2641 ggaatgggaa atgcaaaagg tggagatcag agggcggaat acggaaggcg gaatcctaaa
     2701 taaagaatag acgtatgtga atattattat gtatgtttac ccaatgcaag acctgaagca
     2761 aaggaccaag aaatttgcca ttgattgcat tggcttatgc agaaagatac aatactctcc
     2821 agagtctaag gtcataacca accagctgtt acgtagttca acagcagtag cagctaacta
     2881 cagagcggcc tgccgactta aatccagagc tgattttata gccaagataa ccaccgtgga
     2941 agaagaggca gatgaaagcc agttctggct ggaattaatt caggatgcat ccattttcat
     3001 cgataaagag gtggatcgac tgcaggatga agccggacag ttgacagcca tttttgcatc
     3061 ttccgctaag acagccaaag caaacaatac ccttagaact aaaaaataaa acgactcggt
     3121 tttgtcgatc ttaattccga actccgcatt ccctgcctga cggcaggcag gcgacctccg
     3181 ccttaccatt ataaactcat tcctctaact ttaaggctat gttacatatc aatcccgaac
     3241 aactattcat tttcgacatc gaaactgtgc ccggtgtcaa ggattttaaa gaactatctc
     3301 ccgagttgca ggacttgttc gacgataagg ttggacggca taaggaagag ggagtaagtg
     3361 ccgccgatta ttacaatgcc aaggccggca tatttgctga gttcggcaag gtcatcgtga
     3421 tcagctgcgg ctacatgaaa aagaaaggaa atgggtacga tctaaaggtg aagacctttt
     3481 ccaatcacaa tgagaagcaa ctgctggaag agtttgccca gacgctgatc aatatttcta
     3541 aaagcaacta ctttttgtgc ggacataaca ttcgtgagtt cgatgtgccc tggctttgcc
     3601 ggcgcatgct catcaatggc attcctcttc cgccctgtct ggacctgtat ggcaaaaaac
     3661 cctgggaggt aaaccatgtg gacaccctgg atctctggaa attcggcgac tataaacact
     3721 atacttcgct gcacctgctg agcaccatcc tgggtatccc tacgccaaaa gatgacattg
     3781 atggcagtat ggtaggtgag gtttactgga aacaggatga ccttccgcgt attgccaaat
     3841 actgcagcaa ggatgtggta gcggtggcca atgtgatcct gaaactaaag ggagaaaaga
     3901 tcttaggcga agagcatatc gaactgagct gaaaaggatc tgaagattac tgtactatcg
     3961 gccgaaggca actccaaaaa ccagattccc ctgcaggaag agaaagtgtt gacttgcttt
     4021 gtccagtaca ctggaggtag tgcgaatatc aggcatgtca atgagtcctg tctttacttc
     4081 tgattggatg agaaaccttt caaagaaggt aagctgcaat cctgccatga cttgcagcgc
     4141 ccaaccggcc aggtgaaact catcatatcg ttccatgccc aacagcgttg tatttgttct
     4201 gggaatcagc actccgacgc caccaccttc ggtatgccgg agcgtcactt gtggccattc
     4261 tcccaacaca ctgctgtatc gcaactccag gttcaggtca ttcagtccgt ctgtatgttc
     4321 aaaaaccagg aagtctttgt tgagccgctg agatcgttcg tcatacgttc ctgtgtaggt
     4381 atgatcacct ccaatccagc cgttcattgt aaccacctgt ctctgaacca tgacatactt
     4441 catatgatcg atacccagtg atacattcca gtggtcgttc agataatacc ccagtcggaa
     4501 attatactgt ggaatggtca ttttgaccgg attcagatag ggattgacag aaaactttgt
     4561 ttgcctgtct attgcccgca catcctgcaa agtgaaatca taatcagctc cctgaaaatg
     4621 aatatcgctg cgggtatata cctcccggtt ccagccccag taaatgtacc aggagtctgt
     4681 atgtgaacct cccggcaccg gtattgcaga ctgaccgtat atacctattg atgacaggcc
     4741 tgagaaaagg aacaaaaggg tacacttcca atgcatatgc agatcagaca aggagcgcaa
     4801 gatactgcat ttggaagtag ctaattacct cattcagaaa cgattgccat tttacgtgtg
     4861 atccaaactg ccccgaatac ttcgaacaga ctccacggta ccgccaccgg caggatctga
     4921 actggaagca cccccatatt tccgatgacc acccacatca ttataaaccc tgccagccag
     4981 gataggaaaa ctgtttccat gaatgagaat ttcggtgtga tgatccggat gaaaccggca
     5041 agcagcaaag cccagacacc ccagacggca ccattcaccg gatcggcggg aaagaccaga
     5101 cccatagaag cataatgatc ggtccagtgt tgtgtcaata ccacctgatt tcgaacgaat
     5161 tcatggatgc tgatccagat catagccagc aacacaggaa atattacctt cttcaacata
     5221 tttgatacgt attacaaccc gaagatattt cattggatcc aaacctgacc tgctatccgg
     5281 tttcggggca aaatgcttcc ggttacagaa agtattcgaa cacaaagcgt ttaccgggat
     5341 tttgttagcc taactacact ccagccatac ttc
//
LOCUS       DAOMAX010000183         5255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2624_length_5255_cov_4.241346, whole genome shotgun sequence.
ACCESSION   DAOMAX010000183 DAOMAX010000000
VERSION     DAOMAX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2624_length_5255_cov_4.241346"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..413
                     /locus_tag="PK742_09450"
     tRNA            339..413
                     /locus_tag="PK742_09450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Val,seq:gac)
     gene            623..1381
                     /locus_tag="PK742_09455"
     CDS             623..1381
                     /locus_tag="PK742_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU76854.1"
                     /translation="MMKYFATGLLLMAGGTIFAQTDTTTVVDDEEIVNEMNVGTPDRD
                     RFVFSLHWDGLLGAPDSMDVGPLSRGVGLHFYYDIPFDAAKQYSFAVGLGWNNANYYT
                     KSLLVNDSLGNTNIVGFNESQSVTRNKLAVHYVEVPVEFRFRTKPNQKGNSFKVAVGF
                     KGGYQFSNHTKYVGDEYLIDDANNVTLVDNANEIKYKFYRYSSLSSVQYGPTLRIGYA
                     NVNLEAFYGMAGLFQEGKGPAGTPLQIGVSFNPF"
     gene            1440..2417
                     /gene="meaB"
                     /locus_tag="PK742_09460"
     CDS             1440..2417
                     /gene="meaB"
                     /locus_tag="PK742_09460"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQU76855.1"
                     /translation="MPQPQPNELFTALRAGDTVALSRAITLVESSRAEHRKQAEVLLQ
                     LCLPYAGRSFRIGITGVPGVGKSTLIEQLGLLAIAKDHSVAVLAVDPSSRKTGGSILG
                     DKTRMEKLSVHPRAFIRPSAAGTTLGGVAGSTREAIILLEAAGYDLILVETVGVGQSE
                     TAVHSMTDLFLLLLLSGAGDELQGIKRGIMEMADIVAVNKADGDNVIRADQTRMDFKR
                     ALHLFPPPASGWTVPVLTCSALTGTGMEHLLEQIMDYRTFVNSNGFLERNRSRQLQYW
                     FHESLQQQLMNQLTERFPDDVEAASQAVLEGRELPSAAIAALLNRLFSR"
     gene            2523..2714
                     /locus_tag="PK742_09465"
     CDS             2523..2714
                     /locus_tag="PK742_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQU76856.1"
                     /translation="MNFVSNLNFAEIMVRYTIMMILGIIAGVTHQYWMIIVVMAVFME
                     AIMGFCPVKALFKRNSSKH"
     gene            complement(2810..3157)
                     /locus_tag="PK742_09470"
     CDS             complement(2810..3157)
                     /locus_tag="PK742_09470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQU76857.1"
                     /translation="MISHLIQAFGHAFQGIGHFFRSCRNGRIHLRIAAVVIGLGLILG
                     LSRQDWCTVLLCIALVICLEMANEAIERLADFIQPEQDERIRRIKDVSAGMVLLASIF
                     AAVIGTLVFLAYL"
     gene            complement(3159..4130)
                     /locus_tag="PK742_09475"
     CDS             complement(3159..4130)
                     /locus_tag="PK742_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQU76858.1"
                     /translation="MRLFLRWLSMNKWEVLVSLVMILISVAMLTEPVFLLGALNALRI
                     PYAIFLTAYTVFWFIRNNKLMLSGGVIACLLIFPGIWQYFKPGQVPEQKTVIQREVVR
                     ADSQADLSVVHYNVKENNRYMDRLAEDVLAAGADVVSLQELRPETFPVADTILSAAYP
                     YKRVTLEHKGFGMAIYSRYPIANDSILVAGYFPVMTGTINTPAGEISYLCATTSTPTS
                     EKGFEKQEKEFRLLTQMAKAGSKPLILMGDLNAVPWSTSMEGLLEHGHLEDSRKDLSA
                     TFPSQSVWVQIPIDYILHSPDLKCLAFETLKRSSSNHLGIKGYYQFK"
     gene            4245..>5255
                     /locus_tag="PK742_09480"
     CDS             4245..>5255
                     /locus_tag="PK742_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76859.1"
                     /translation="MKTLMRSLALAAAILVTPSLKAQVQCDNDTSNFVSLVDLQTGYY
                     LGLYQGGLYPGGSNFLPASHRADGLAIAKAIKPLDTLGNVDWENGKVIFAGMGASTAG
                     NSWNHFVDIVDTASGLNPCMRAINACLGAKGLDIMIDTAVNGWYWYDEILPKIEYYNS
                     SRYQVQVAWIKSASKADTILEFPLFPNAIADKYEALMPILLDTFPNIKLVFISGFFYG
                     GYADPLKEFYDVVVEPGSYWTNFAVKWVIERQITGDPDLAFKGPGRRSPWIAWGPHVW
                     ADGMRANEWDGLQWNCEDDFAWDGGGYHLTNTGKKKEADLLFTWAKTNPVTRRWFLDG
                     PQW"
BASE COUNT         1308 a         1252 c         1351 g         1344 t
ORIGIN      
        1 gtaactcgaa gttctttagg ttactggccc acctgggcga gaagtttatc ccgctttaca
       61 gatacggcgc agcgaagcaa gtcagtagct gttctgcggg gagcccaggg tagggttacg
      121 agagtgggag agttggagag tcgagtgtga gtggctagag aaggttactc aaagttctta
      181 aggtaactgg cccacctggg cgagaagttt atcccgactt acagatacga cgaagcggag
      241 cgagtcagta gctgttctgt cggggagccc tgggtagggt taggagagtg ggagtgatac
      301 tccaggtact ccagcttcag actcaaacta ttttatccgg gcgattagct cagttggttc
      361 agagcgcttg tttgacagac aagaggtcgg cagttcgaat ctgccatcgc ccacaacgaa
      421 gatccgagaa tagagtttga gtgatgaggg agtgctactg atagctatcg gtagcggggg
      481 atatggcctg gatgattttc acaatgagtc catcatgatc tatggtattg gaacgcctgg
      541 gtaattcaaa caatagtaat tgcgctctgc cagtaacatt tcacctattt ttgcgtctta
      601 ttggtaacca accattttga agatgatgaa gtattttgcc actggtctgc tgttaatggc
      661 aggaggaaca attttcgctc aaacagatac caccacagtt gttgacgacg aagagatcgt
      721 caatgagatg aatgtgggta cgcctgatcg tgatcgtttt gtattcagcc tgcattggga
      781 cggactgctg ggtgcacctg attctatgga tgtgggcccg ctgagccgcg gggtcggtct
      841 ccatttctac tatgatattc cgttcgacgc tgccaagcag tacagctttg cagtcggcct
      901 tggctggaac aatgccaact actataccaa atcattgctg gtcaatgact cacttggaaa
      961 caccaatatc gttggattca atgagtcgca atcggtcacc aggaataaac tggctgtgca
     1021 ttatgtagaa gtgccggtag aatttcgctt tcgcaccaaa cccaatcaaa aaggtaattc
     1081 tttcaaagtg gctgttggtt ttaaaggcgg ataccagttc agcaatcata ctaagtacgt
     1141 aggagatgaa tacctcattg acgatgccaa caacgtgacc ctggtggaca atgccaatga
     1201 gatcaagtat aaattctaca gatattcttc tctcagctct gtacaatatg gcccaacact
     1261 gcgcatcggc tatgccaatg tgaatctgga agctttctat ggtatggcag gtctgttcca
     1321 ggaaggcaag ggtcctgctg gtacaccttt gcagatcggt gtgtctttca accccttctg
     1381 atccaaacga acattacagg cagtccttcc catccgggaa gggttgcgta tttttaggca
     1441 tgccgcaacc gcaaccgaat gagttgttca ctgcactccg ggcaggagat accgtggctt
     1501 tgtcaagagc catcaccctg gtggagagca gccgggctga gcaccgtaaa caagcagagg
     1561 tgctgttgca gctatgtctg ccctatgcag gcaggtcctt tcgcatcggt attaccggtg
     1621 ttccaggggt tggcaaaagc accctgatcg agcagctcgg attactggcc atcgcaaaag
     1681 atcactcggt tgctgtgctg gcagtggatc ccagcagcag aaagacgggt ggtagtatcc
     1741 tgggcgacaa gacccggatg gaaaaactgt cggtccaccc cagggctttt attcgtcctt
     1801 ctgccgcagg cactaccctg ggtggtgtgg caggcagtac ccgggaggcc atcattctgc
     1861 tggaagctgc cggatatgat ctgatcctgg tggaaaccgt tggtgtgggg caaagtgaaa
     1921 cagctgtaca cagcatgacc gatctctttc ttctcctgtt gctttcggga gcaggcgatg
     1981 aattgcaggg catcaaaaga ggcatcatgg agatggcaga tattgttgcg gttaacaaag
     2041 cagatggtga taatgtgatt cgtgcagatc agacccggat ggattttaaa cgtgcattgc
     2101 atttgtttcc gccacctgcc tcaggctgga cggtgccggt ccttacctgc tcagcactga
     2161 caggaacagg tatggaacat ttgctggaac agatcatgga ctacagaaca tttgtaaaca
     2221 gtaatggctt cctggagcga aaccgcagcc ggcagctgca atactggttt catgaatctt
     2281 tgcagcaaca actaatgaac cagctgaccg agcggtttcc tgatgatgtg gaagctgctt
     2341 ctcaggctgt gctggagggc cgcgaattac cttcagcagc tattgctgca ctcctgaacc
     2401 gcctgttttc ccgataaggg tcagcctccc ggaattgctt aaagtcaccg ttttttctga
     2461 cagaggtcat gcctctgccc gatccatctt tgaaacttta ctccatcaaa accgaactaa
     2521 cgatgaattt tgtaagcaat ctgaacttcg ctgagatcat ggtgcgctac accatcatga
     2581 tgattcttgg aatcattgca ggtgttaccc atcaatactg gatgatcata gtggtcatgg
     2641 ccgtcttcat ggaagccatc atggggttct gccccgtcaa agccttgttc aaaagaaaca
     2701 gcagcaagca ctaaacgaac accgtgtcaa acggtgggca acatactttt acggttgtct
     2761 ttctttgagt atgcctgaat ccgtcccggt ctttccggga cggttttttt tacagataag
     2821 caaggaaaac gagtgttccg atcactgccg caaaaataga tgccagcaat accataccgg
     2881 ccgaaacgtc tttgatcctt ctgatacgct catcctgttc cggttgtatg aaatcagcaa
     2941 ggcgctcaat ggcctcattg gccatttcca ggcagatgac cagcgcaatg catagcagaa
     3001 ccgtacacca gtcttgtctg cttaagccca ggatcaatcc cagcccaata accacagctg
     3061 ctatacgaag atgaatacgg ccatttctgc agcttctgaa aaagtgacct ataccttgaa
     3121 aggcatgacc gaaggcctgt atcagatgag atatcatatt atttgaattg gtaatatcct
     3181 ttgatgccca ggtgattgga actggatcgt ttgagtgttt caaatgccag gcatttcagg
     3241 tcggggctgt gcaggatata atcgatcgga atctgcaccc agacagactg gcttggaaag
     3301 gtggccgaca ggtctttccg gctgtcttcc agatgtccat gttccagcag gccttccata
     3361 gaagtgctcc agggcactgc attgagatcg cccatcagaa taagtggctt gcttcctgcc
     3421 ttcgccatct gggtgagcag tcggaattcc ttttcctgtt tctcgaagcc cttttcgctg
     3481 gtgggcgtgg aagtggtggc acacagataa gagatctcac ctgccggagt attgatggta
     3541 cctgtcatga ccggaaagta tcctgctacc agaatactgt cattagcaat gggataccgg
     3601 ctgtagatgg ccatgccaaa gcccttgtgc tccagggtga cccgcttgta aggataggcg
     3661 gcactcagga tggtgtcggc aaccggaaag gtctccggcc tgagttcctg cagggagacc
     3721 acatctgcac cggcagccag cacatcttct gccaggcggt ccatatatct gttgttctcc
     3781 ttcacattgt agtgaaccac gctgagatct gcctgagagt ctgcccgcac tacctcccgc
     3841 tggatgacgg tcttttgctc gggtacctgc ccgggcttga aatactgcca gatgcccggg
     3901 aatatcagca ggcaggctat caccccccca ctcagcatca gtttgttatt tcggatgaac
     3961 cagaataccg tataggcggt cagaaaaatg gcatatggaa ttcggagggc attaagtgca
     4021 cccagcagaa ataccggttc agtgagcatg gccacactga tcaggatcat gaccagtgaa
     4081 accagcactt cccacttgtt catagataac cacctcagaa acagtctcat atcttcatta
     4141 gagggttaat aaaaggaata gaattgtcag atttactcct aaaacacagc tcatgcaaca
     4201 aaattcctaa ttttgacgac taatttccgg aacaccgcca tatcatgaaa actcttatgc
     4261 gctctctggc tctggcagct gccatccttg tcacaccttc tctgaaggct caggtacagt
     4321 gtgataacga cacatccaac tttgtttcac tggttgacct gcaaacaggc tactacctgg
     4381 gtttgtatca gggtggtctg tatccgggcg gaagcaattt cctgcctgcc tcacaccgtg
     4441 ccgatggtct ggccattgcc aaagctatca aaccacttga taccctgggc aatgttgact
     4501 gggaaaacgg caaggtgatc tttgccggta tgggtgcttc cactgcagga aactcttgga
     4561 accactttgt tgatattgtt gatacggcct caggattgaa cccctgtatg cgggccatca
     4621 atgcctgtct gggtgcaaaa ggactggata tcatgatcga tacagcagtc aatggctggt
     4681 attggtacga tgagatcctc cctaagatcg aatactataa ttcttcccgt tatcaggtgc
     4741 aggtggcgtg gatcaaatcg gcctccaagg ctgataccat tctggagttc ccgttgtttc
     4801 cgaacgctat tgcagataag tatgaagctt tgatgcccat acttcttgat actttcccaa
     4861 acatcaaact ggtatttatt tcaggcttct tttatggtgg atatgctgac ccattaaaag
     4921 aattttatga tgttgttgtg gagccgggaa gttactggac aaacttcgcc gtcaaatggg
     4981 ttattgaaag acagatcacc ggagatcctg atctggcatt caaaggaccg ggcagacgtt
     5041 ctccctggat cgcatgggga ccgcacgtct gggctgatgg tatgcgtgcc aatgaatggg
     5101 atgggttaca atggaattgt gaagatgatt ttgcctggga tggcggagga tatcacctga
     5161 caaataccgg taagaagaaa gaagcagacc tgttattcac ctgggcaaaa accaatcctg
     5221 taacccgccg gtggtttctt gatggtccgc agtgg
//
LOCUS       DAOMAX010000184         5254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2626_length_5254_cov_3.339488, whole genome shotgun sequence.
ACCESSION   DAOMAX010000184 DAOMAX010000000
VERSION     DAOMAX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2626_length_5254_cov_3.339488"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="nqrA"
                     /locus_tag="PK742_09485"
     CDS             complement(<1..1165)
                     /gene="nqrA"
                     /locus_tag="PK742_09485"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HQU76860.1"
                     /translation="MSRLIRLTKGYDIKIKGAPVGKLDSSFSAKTFSVKPVDFHGLSP
                     IPKLFVSEQTEVKAGDPLFYDKSDERIHYCSPVSGEVVAIHRAEKRAIDEIVILADSD
                     IRYRTWEPADPNTLTREAIIERMLESGAWLFLRQRPYNVVASPDEAPKAIFISGFDSS
                     PLAPDMAMVMEGKEEAFQTGIRILSKLTSGKIHLSLSARQRPPKVFDTVQGVEIHWFD
                     GPHPTGNVGVQIHHIDPVNKGEVVWHIHPEDVANLATLFTKGIYSPERTVAVAGPMVS
                     KPHYVKTLQGACIEGLVSNNLISDHVRCISGNVLTGRTVASNGHLGFFDHLVTVIEEG
                     DHAELLGWLFPSYARPTRSMAYWAYANEAPDGYAVTTSMHGEERAFVMSGQYEQ"
     gene            1325..2584
                     /locus_tag="PK742_09490"
     CDS             1325..2584
                     /locus_tag="PK742_09490"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HQU76861.1"
                     /translation="MFQNLFAILLALCSTITGAQTDRLHQNNTSYLRTVLLQRSDLEM
                     SMPIIKIGSGDQLVLRFDDLRSGVHDYSYTFEHCNQDWTTSGLFPLDYLDGFEEQFIS
                     DYAFSFNTKQSYTQYRAVFPNDQLNFLVSGNYLIHIWESGNDSAPVLTLPFYVREELA
                     TIQASVQQPNLTRFRNDYQRLLLEVGIKEVPSSNPFDEVRVSVIQNFRENQPLTDIKP
                     RLIANNTMLFDQTDLVFPGGKEFRRFDIRSTRVQSERIVKMDRTQTGTDAWINVDQNR
                     SYQRYAFDNDVDGQFLIDADMARNKHLDADYVMVHFALESPFWMSQGDYFVVGGFNHY
                     QTGEENRMEYDADLQQYTCQIYLKQGYYNYLYTFLEHGSNIQDYSMAEGNYFETENNY
                     QVMIYLHDNMRNADRLIGYSSLTSFPR"
     gene            3113..3547
                     /locus_tag="PK742_09495"
     CDS             3113..3547
                     /locus_tag="PK742_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76862.1"
                     /translation="MAKTTKKAAPKKAAKKAAPKKAAKKAAKKAAPKKAAKKAVKKAA
                     PKKAAKKAVKKAAPKKAAKKAAKKAAPKKAAKKAAKKAAPKKAAKKAAKKAAPKKAAK
                     KAAKKAAPKKAAKKAAKKAAPKKAAKKAAKKAAAPDNSTPAS"
     gene            3752..>5254
                     /locus_tag="PK742_09500"
     CDS             3752..>5254
                     /locus_tag="PK742_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76863.1"
                     /translation="MKKGIVHFLLCCSCSMILAQAPEKNFFFETRSSANTSHSEIRSA
                     EQYDHHQSDRSDQLSHKRSSAEVIPFGYYLTVYSMLLDGQNQVSYHPDINSVIFIHRS
                     HYYIQGISFDVSTDGGATWQLKNEISPDCAAGEAPALANKYPGATIYNPPGNTDPDQA
                     FIVANGPALDPDAPGNWGYTFRISATLDGEHLDEQYYQTPDEPFDYHPLGIDYCSSGA
                     MWDVSTTFNNTGNADLNDLNFDKYRINKITPNEAGTGFDYSTTTMEPNFYTYVPVGAA
                     NEENYAGLGWGVHFDPTGTIGYLVILGGLDDGPTTISPVIYKSEDAGESWNLLPVSDF
                     STLTTFEENTPPTWDGTRVPFFTNYDAVVDSNGVLQMITEFTGRTTTSTDPDSLYSIW
                     QNYSGFTHLSVSDGSDWNANLLGYSNLLRDSLNCGYPELFAIPYRVQASRNSAGTRVF
                     FTYTMTDSSYHTYHQAPDLMVTGYNVMTNEYTWTKNYSSGTDAEGEVYFPV"
BASE COUNT         1521 a         1250 c         1117 g         1366 t
ORIGIN      
        1 cctgttcata ctgacccgac attacaaagg ctctttcttc tccatgcatg gaagtagtga
       61 ccgcataacc gtcaggtgct tcattggcat aggcccagta ggccatagat ctggttggtc
      121 ttgcataact ggggaaaagc catcccagca attcagcgtg atcaccttct tcgatcactg
      181 tgaccagatg gtcaaaaaaa ccaaggtgac cattgctggc tactgttctg cctgtcagca
      241 cattaccgct gatacagcga acatggtcag aaatcagatt gttgctcaca agcccttcaa
      301 tgcaggcacc ctggagggtt ttaacataat gaggcttgct taccatagga ccggccactg
      361 caacagtgcg ctccgggctg taaatacctt tagtaaataa tgtggcgagg ttggcaacat
      421 cttccggatg aatatgccat accacctctc ctttattaac aggatctata tgatggatct
      481 gcacaccaac atttccggtt gggtgcggac cgtcaaacca atgtatttcg accccttgta
      541 cggtatcaaa taccttaggt ggcctctggc gggcagacag actcagatgt atctttccgg
      601 aggtaagttt ggacagtatc ctgattccgg tctggaatgc ctcctcttta ccttccatga
      661 ccatagccat atcaggagcc agaggtgaac tgtcaaaacc gcttatgaat atggccttgg
      721 gtgcttcatc cgggctggct accacattat aagggcgctg ccgaagaaac aaccatgcac
      781 cggattccag catgcgctca atgatcgctt cgcgggttag ggtattcggg tcagccggct
      841 cccaggtgcg atagcgaata tcactgtcgg ccaggatcac gatctcatca atggctcttt
      901 tctctgccct gtggatcgca actacttctc cagaaaccgg agaacagtag tgaatgcgtt
      961 catcagattt gtcgtaaaac agcggatcgc cggctttaac ctctgtctgc tcgctgacaa
     1021 agagcttggg aataggcgaa agcccatgga aatcaaccgg tttaacggaa aatgttttgg
     1081 cgctgaaaga gctgtccagt ttgccgaccg gggcaccctt gatcttaata tcatacccct
     1141 ttgtcagccg tatcaatctg ctcatgctgg taagctgttt ttgaaaaccg cacaaagtta
     1201 tactattcaa tatggaactt ggtaaagttc cggatgattt caacacttta tcccaacccc
     1261 ggcccggaag acttctgcgg cagggttttt ggggttacct tacataaaga ctattcttgt
     1321 gaccatgttc caaaacctgt tcgccatcct gctggccttg tgcagcacaa taaccggtgc
     1381 gcaaaccgac cggttgcacc aaaacaacac ctcttatcta cgcacggtat tgttacagcg
     1441 aagcgatctg gaaatgagca tgccgatcat caagattggc tccggcgatc aactggtgct
     1501 tcgttttgac gacctgagaa gcggggttca tgattacagc tacacatttg aacattgcaa
     1561 ccaggactgg accaccagtg gtctgtttcc gctcgattac ctggatggct ttgaagaaca
     1621 attcatcagt gactatgcct tttccttcaa caccaaacag tcatacacac agtaccgtgc
     1681 ggtctttccg aatgatcagc tgaattttct tgtttccggc aactacctta ttcatatatg
     1741 ggagtctggc aatgactcag ctcctgtact taccctcccc ttctatgtca gagaagaact
     1801 tgctaccata caggcatctg ttcaacaacc aaatcttacc cgcttcagga atgattatca
     1861 aagactgctt cttgaggtgg gcatcaaaga ggtacccagc agcaatcctt ttgatgaagt
     1921 cagggtctcg gtcattcaga actttcgtga gaaccaaccg ctaactgata tcaaaccaag
     1981 actaatagca aacaatacca tgctttttga tcagacagac ctggtatttc ccggaggaaa
     2041 ggaattcagg cgttttgata tcagaagtac ccgggttcaa tcagaacgca ttgtaaagat
     2101 ggacaggacc cagaccggca ccgatgcctg gatcaatgta gatcagaaca gaagctatca
     2161 acgctatgct tttgacaacg acgtggacgg acaatttctg atagatgccg acatggcacg
     2221 aaacaaacac ctggatgcag attatgtcat ggtgcatttt gcattggaaa gccccttttg
     2281 gatgtcgcag ggagactatt ttgtggttgg gggtttcaac cattatcaga ccggcgaaga
     2341 aaacagaatg gaatatgatg cagatcttca gcaatatacc tgtcagatat atctgaagca
     2401 gggctactat aattatctct ataccttcct ggaacatgga agcaacatac aggattacag
     2461 catggccgaa ggcaactatt ttgaaacaga gaacaattat caggtcatga tatacctgca
     2521 tgacaatatg cgaaatgcag acagactgat aggctacagc agcctcacct cctttccaag
     2581 ataacagtaa atacctgtat gcgatattgc ttttttgacc aactcatcag aaggttttga
     2641 aggcaacgaa tcagaacctg ctccgtccgg aaggccagaa tatctcctga tcctttcaaa
     2701 tgcttgccgt ccgctaaaaa aatatttaca ggagaccgct cttttcaacc gtcaatgcac
     2761 atggccatgt tgctgtatgt tcagcgcttt atgcgctggt cattctgctt tatttatatt
     2821 cttatcacca gttcaataaa caacacaata cactaaagaa atgctgtgaa aggcttattt
     2881 tactgatgtt tcataatatt attgaaattc ttatccttaa atgaggaagt gcaatatcct
     2941 gtagaagcac acaagtatca gtcatcagag aagtaaaccc gtgcgtcaaa aaatcccgtg
     3001 gccgtgtaca gagaaatatt cagcaggaca atcctatcaa caaaaaaagt tctttaattc
     3061 tttggtaaaa tgattttgta tttaattttg cgataaccaa aacatttttc caatggcaaa
     3121 aacaactaaa aaagccgctc ctaagaaagc cgctaagaaa gctgctccta agaaggctgc
     3181 caagaaagct gcaaaaaaag ctgctcctaa gaaggccgct aaaaaagcag taaagaaagc
     3241 cgctcctaaa aaggccgcta aaaaagcagt aaagaaagcc gctcctaaga aagccgctaa
     3301 gaaagccgct aagaaagctg ctcctaagaa agccgctaag aaggctgcta agaaagctgc
     3361 tcctaagaaa gctgctaaga aggctgccaa aaaagctgct cctaagaaag ccgccaagaa
     3421 ggctgccaaa aaagctgctc ctaagaaagc tgctaagaag gctgctaaaa aagctgctcc
     3481 taagaaagcc gctaagaagg ctgccaaaaa agctgctgct ccagataaca gcacaccagc
     3541 aagctaagcg acatctgaac aaaaaatcag caaggagcct tcgggctcct tttttttgcg
     3601 cttacctgca gatgcatgca cacctttgtc ccttcaactc cttttcatca gatacttctt
     3661 ttgtcaaaga tcctgaccag gatatgattc cttgttttca ccagactcac taattacaaa
     3721 caggatatct ttataaagaa tcagcctgct catgaaaaaa ggcatcgtac attttttgtt
     3781 atgctgttcc tgcagcatga tattggcaca agctccggaa aagaatttct ttttcgaaac
     3841 aaggtcttct gcaaacactt ctcattccga aattcgttcg gcagaacagt atgatcacca
     3901 tcagtcagac agatcagatc agttatctca taaaagatct tctgcagagg taatcccttt
     3961 tggttactat ttaactgttt attccatgtt gcttgatggg cagaatcagg tttcctatca
     4021 tcctgacatc aacagtgtga tcttcattca caggagccac tattatatcc agggaatttc
     4081 ttttgatgta tctacagacg gcggcgctac atggcagctc aaaaatgaaa tttcaccaga
     4141 ctgtgccgca ggtgaggcac ctgctcttgc caacaagtat cctggtgcga ccatttataa
     4201 tccaccaggg aatacagacc ccgatcaggc atttattgta gccaatggtc cggcactgga
     4261 tcctgatgca ccgggcaatt ggggatatac attcagaata tcagccaccc tggatggcga
     4321 gcatcttgat gaacagtatt atcagacacc ggatgaacca tttgattatc acccgttggg
     4381 tatagactac tgcagttccg gggcgatgtg ggatgtatct actactttca acaataccgg
     4441 caatgccgat ctgaatgatc tgaattttga taaataccgt atcaacaaaa tcactcccaa
     4501 tgaagcgggt accggatttg attattccac taccaccatg gaacccaatt tttataccta
     4561 tgtaccggtt ggtgctgcaa atgaagaaaa ttatgccgga cttggatggg gggttcattt
     4621 cgatccaacc ggaacaatcg gatatctggt cattctcggg ggtctggatg atggccccac
     4681 aaccatatct cctgtcatct ataaatccga agatgctggt gaatcatgga acttgttgcc
     4741 tgtatctgac ttcagcacct taaccacctt cgaagaaaat actcctccta catgggatgg
     4801 tacgagagtg cctttcttca ccaactatga tgctgtagtt gacagcaacg gagtgcttca
     4861 gatgatcact gagtttacag gcagaaccac aaccagtacc gatcctgatt cgttgtattc
     4921 gatctggcag aactattcag gattcaccca tctgtcagta agtgatggca gtgactggaa
     4981 tgcaaactta ctgggttaca gcaatctgct aagagattca ctgaattgtg gttatcccga
     5041 attgtttgcc ataccttatc gtgttcaggc cagcagaaat agtgcaggaa ccagggtctt
     5101 tttcacttat accatgaccg attcatctta ccatacatac caccaggctc cggatctgat
     5161 ggttacggga tataatgtca tgacaaatga atatacatgg accaaaaatt actcttccgg
     5221 aactgatgct gaaggtgagg tatattttcc ggta
//
LOCUS       DAOMAX010000185         5196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2674_length_5196_cov_4.199767, whole genome shotgun sequence.
ACCESSION   DAOMAX010000185 DAOMAX010000000
VERSION     DAOMAX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_5196_cov_4.199767"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK742_09505"
     CDS             <1..241
                     /locus_tag="PK742_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76864.1"
                     /translation="DMCEALLQETGVAILPGAAFNRPANEFTARLATVNFDGAKALAK
                     CETIPLDTPLPDSFTKTYCKETLDACKRIVKWLHD"
     gene            complement(230..799)
                     /locus_tag="PK742_09510"
     CDS             complement(230..799)
                     /locus_tag="PK742_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76865.1"
                     /translation="MDPEDLLSGNEHPAIPYHQEILVRDDFWQKQQEEWMAAGGFEQF
                     LCLIKEAFLDGTLTEDHFRSLTGAMSDGWMIKWHAHRFSDDTLSRCADALRDVILPLG
                     YTLYMSDLREFLRGDYVETIERHYLKPALVIKEGLADQLYGNILIEHRLIDRASYDLR
                     MVVQYYHDQNFAYPQPFVKLLNTLCRSVM"
     gene            850..1809
                     /locus_tag="PK742_09515"
     CDS             850..1809
                     /locus_tag="PK742_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQU76866.1"
                     /translation="MNKPRILITGAAGFLGSHLCDRFIREGYQVIAMDNLLTGSMRNI
                     EHLFPNEDFSFYHHDVTRFVHVPGKLDFILHFASPASPIDYLKMPIQTLKVGSLGTHN
                     LLGLAKEKNARILIASTSEVYGDPMVHPQKESYWGNVNPVGPRGVYDEAKRFMEAITM
                     AYHNYHGLDTRIARIFNTYGPRMRLNDGRALPAFIGQALRGEDLTVFGDGSQTRSFCY
                     VDDLVEGIYRLLLSEYPEPVNLGNPDEISIRDFAEEIIALTGTKQKVVYKPLPVDDPQ
                     QRQPDISLAKEKLNWQPKVNRQDGLRITYEWFRQLPESALHEL"
     gene            1869..3497
                     /locus_tag="PK742_09520"
     CDS             1869..3497
                     /locus_tag="PK742_09520"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76867.1"
                     /translation="MLPRVQTILFLFCCSCLGTSAQAQFEILDVNNIGFGVGYGGTLC
                     NHDDVYYSLAPIEGTNHIIWGIAPLWSAYDDSGQVYGDILYINNAEDHAGPHADTALN
                     LAQELALQRYKMVRKIERSEIEAFTSHFGEPGYTIPQIIRDWPGNGDTTLGTTFQLAP
                     YVDHNANGIYEPDSGDYPLIKGDQAIFQLLHDRPTGSQATGGDPNFSMLEYRQMVYGY
                     EALPGSLLDNAVFIDMQIINRSDRTYHDFVFGYLTFFELGFFGNNYMGSDSSVNLQYC
                     YKGDTTDTGYGDWIYYGDLLPATGLQFVNVPMDVSMNPMSELSESSLQRFQFYQRAYW
                     PFDTTHMTDGGSGYGTGSLTNYSYHDHPDDTDGWNEFTQGNPPGQRRAYSSTFFGDLH
                     PGDTVCHTAAVLFAPGTLPNNRTAAVTDLINMASALQAQYPYLPDAGNCMQTRQEAPY
                     WQTEVNPADGITFLPYPNPASDVVYLHTGPFKQSDVSLNLSDMNGKLIETWFLADLNS
                     EQIISIRLPALTAGIYVLSGQANGFTFVKKIVIK"
     gene            3494..5119
                     /locus_tag="PK742_09525"
     CDS             3494..5119
                     /locus_tag="PK742_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76868.1"
                     /translation="MKRNLLALVFVSICIAGNSQNSVTLDINNISLTQVYPGSIGYSY
                     VSYPSLFIAPTGSGRSPMIGAFYWFRGIDIYDQNRVYAANYWNISDQFVGPSHNQFLD
                     PESEAAFYSLYNKNWYIHRATIDSFIERRGEPDYVIPEPILNWPGNGNPELGTAERLA
                     PFADANGDGYYDPYTGDYPLIKGDAAIFQIMNADIDSESDYYRRMGIEMHSMFYAYEA
                     DDQSVLGNCIFVHNTIINRSWFDYKEFQTGLYIDFELGAYIDDFTGCDSALSLTYVYN
                     GDSIDGPDPLGYGDMIPAFGIQQLSHPMYACGFNSIDVNFFYGYYYPFSYFMQGYWPI
                     NFLDFYSFPFTYGNNGIWGTTQTRYLYSDRPDNPDGWSMHALDYLPGDMNSMSSITPV
                     ELAHGDTLCTEFALLFAPAEVPNDPLHALNKLFLNAEILRAMYPSLSNGEECIAYRWE
                     PWNAPDADGITFLPYPNPASDVVYLHTGPFHDTELIVELRDMTGQLVARSTYEGILEN
                     QTLSFPLPALARSQYVLSGLVDGHVFSKQIQVW"
BASE COUNT         1373 a         1278 c         1201 g         1344 t
ORIGIN      
        1 ggatatgtgc gaggcattgt tgcaggaaac cggcgtggcc attctgccgg gtgcagcctt
       61 taaccggccg gccaatgaat tcactgccag actggcaacg gtcaattttg atggggccaa
      121 agcgctggca aagtgcgaga ccattccgct tgatacacca cttccggact ccttcaccaa
      181 gacctattgc aaggaaactc tggatgcctg taagcgcatc gtaaagtggt tacatgactg
      241 accgacacag ggtgttcagc agtttcacga agggctgcgg ataagcaaag ttctggtcgt
      301 ggtaatactg gaccaccatg cgcaggtcat atgaggcacg gtcaatgagt ctgtgttcga
      361 tcagaatgtt gccatacagc tgatccgcca gcccctcctt gataacaaga gctggtttga
      421 gatagtggcg ctcgatggtt tctacataat cgccgcgaag aaactcacgc aggtctgaca
      481 tatataaggt gtagcccagg ggcaaaatca catcacgaag ggcatctgca caacgggaaa
      541 gagtatcatc agaaaaccga tgtgcatgcc atttgatcat ccatccgtca gacatagcac
      601 cggtgagcga ccggaaatga tcttctgtca gtgtgccatc cagaaaagct tctttgatca
      661 gacaaaggaa ttgttcaaat ccccccgctg ccatccattc ttcctgttgc ttttgccaga
      721 aatcatcacg taccagaatt tcctggtgat acggaatggc aggatgctcg ttgccggata
      781 acagatcttc agggtccata caaagggtac tccctgcaaa ggtgtatttt tgtgcgcaag
      841 ttttctgatt tgaataagcc ccgcatactc ataaccggtg cagccggatt tttaggttcc
      901 catttgtgcg accggttcat ccgggaggga taccaggtca ttgccatgga caatctgctg
      961 acaggcagca tgcgcaacat cgaacacctg tttccaaacg aagacttttc cttttaccac
     1021 cacgacgtga cccgtttcgt tcacgtgcct ggtaagttgg atttcatcct ccatttcgct
     1081 tcaccggctt cccccataga ctacctgaag atgcccattc aaacactgaa ggtgggaagc
     1141 ctgggaacac ataacctgct gggacttgcc aaagagaaga acgcacgcat cctcatcgcc
     1201 tccacttctg aagtctatgg cgatccgatg gtgcatccgc agaaagaatc ttactggggt
     1261 aatgtgaacc ctgtcggacc cagaggcgtc tatgacgaag ccaagcgttt catggaagcc
     1321 atcaccatgg cctatcacaa ctaccacgga ctggataccc gcattgcccg catcttcaat
     1381 acctatggac cacggatgag actcaatgac gggcgtgcgc ttccggcatt catagggcag
     1441 gcattgcgtg gggaagacct gaccgttttt ggcgatggaa gccagacccg ttccttctgt
     1501 tatgtggatg atctggtgga aggcatctat cgcctgttgc tgagcgaata ccctgaaccg
     1561 gtgaatctgg gtaatccgga cgagatcagc atccgtgatt ttgccgaaga gatcatcgcc
     1621 ctgaccggca cgaaacagaa agtggtgtac aagccgcttc cggtcgatga cccgcagcaa
     1681 agacaaccgg atatttccct cgcaaaagaa aaactgaact ggcaacctaa ggtgaacaga
     1741 caggatggtt tgcgcatcac ctatgaatgg ttcagacaac tacccgaatc tgcgttgcac
     1801 gaattgtaat atggttgtca gaacctgacg atcattcttt cacaactttt tttttcttat
     1861 ttttaatcat gctccctcgt gtacagacca ttctgttcct gttctgctgc agctgcttag
     1921 gaacatcggc tcaggctcag ttcgagatac tggatgtcaa taacatcggt tttggagttg
     1981 ggtacggggg caccctgtgc aatcatgacg atgtgtatta tagtctggca cccatagagg
     2041 gaacaaacca catcatctgg ggaatagccc cgctttggtc agcctacgat gacagcggcc
     2101 aggtctatgg cgatattcta tatatcaaca atgctgaaga tcatgcaggt ccccatgcag
     2161 acaccgcact gaacctggca caggagctgg cacttcagcg ttacaaaatg gtcagaaaaa
     2221 ttgaacgaag cgaaatagaa gcatttacat ctcattttgg tgaaccgggc tataccatac
     2281 cacagatcat cagagactgg ccgggcaacg gagacaccac attaggcaca acgtttcagc
     2341 ttgctcctta tgttgaccac aatgcgaatg gtatctatga accagacagc ggcgattacc
     2401 ccctgatcaa aggagaccag gccatcttcc agctcctgca tgacaggcca accggcagtc
     2461 aggccaccgg gggcgacccg aacttcagca tgctggaata ccggcagatg gtgtacggat
     2521 atgaagcgct gcccggatct ctgctggaca atgcagtttt catagacatg cagatcataa
     2581 accggtctga ccgcacctac cacgattttg tattcggcta cctgaccttc tttgaactgg
     2641 gatttttcgg taacaactac atgggtagtg acagctctgt gaatctgcaa tactgttata
     2701 aaggagatac aactgataca ggttatggag actggatcta ttacggcgat ctgcttcctg
     2761 caaccggatt gcagtttgta aatgttccga tggatgtatc tatgaaccca atgagtgaac
     2821 tcagtgaatc atcgctgcaa cgattccagt tttaccagag agcctactgg ccctttgata
     2881 caactcatat gactgatggt ggaagcgggt atggaaccgg aagtctgacc aactatagtt
     2941 atcatgatca tccggatgac acggatggat ggaatgaatt cacacaaggc aacccacccg
     3001 gacaaaggag ggcatatagt tccaccttct tcggagacct gcaccccggc gataccgtct
     3061 gtcatacagc tgccgtactt tttgcaccgg gaacccttcc aaacaaccgc acagctgctg
     3121 taacagacct gatcaatatg gcctcggccc tgcaggcaca atacccttat ctacccgatg
     3181 ccggaaactg catgcaaacc cggcaggaag ccccttactg gcaaacagaa gtaaatccgg
     3241 cagatggcat cactttcctc ccctatccaa atcctgccag tgatgtggtc tatctgcaca
     3301 ccgggccgtt taaacaatca gatgtgtcac taaacctttc tgatatgaac ggcaaactta
     3361 tagaaacatg gttcttagca gacctcaatt ctgaacagat catcagtatc agacttcctg
     3421 cattgacagc cggcatatat gtattaagtg gtcaggcaaa tggattcaca tttgttaaaa
     3481 aaatagttat aaaatgaaga gaaatctgct ggcattagta tttgtctcta tttgtatagc
     3541 cggcaattcc cagaatagtg ttacactgga tattaataat atctcgctca ctcaggtcta
     3601 tcccggatca ataggctata gttatgtatc atatccgagt cttttcattg ccccaacagg
     3661 ctcaggccgg agccctatga ttggagcctt ctattggttt cgtggtattg atatatacga
     3721 tcaaaacaga gtttatgcag caaactactg gaacatcagc gatcaatttg ttggcccgtc
     3781 acataatcag ttccttgatc cggaatctga agcagcattc tattccttgt acaacaaaaa
     3841 ctggtacata cacagggcta ccattgattc ttttatcgaa agaagaggag agcctgacta
     3901 tgttattcct gaaccaattc tgaactggcc tggaaatggc aaccctgagc tgggaaccgc
     3961 tgagcgtctg gctcctttcg cagatgccaa cggagatgga tattacgacc cctatactgg
     4021 cgactaccca ctcatcaaag gtgatgccgc tatttttcag atcatgaatg cagatattga
     4081 ttctgaatcg gattattatc gaagaatggg gattgaaatg cattccatgt tttatgctta
     4141 tgaagcagat gaccagtcgg ttctgggaaa ttgcattttc gttcataata ccatcatcaa
     4201 cagaagctgg tttgattata aggagtttca aaccggcctg tatatagatt ttgaactggg
     4261 ggcatatatt gatgatttta ctggttgcga cagcgcacta tccctgacct atgtatataa
     4321 tggcgattct attgacggac cagacccatt aggttatggt gatatgatac ctgcatttgg
     4381 aattcagcaa ttaagccacc ctatgtacgc ttgtggattt aatagcattg atgtgaattt
     4441 tttctatggc tactactatc ctttttctta cttcatgcag ggatactggc ctataaattt
     4501 tttagacttc tactccttcc cgtttactta tggtaacaac ggaatctggg gtactacaca
     4561 aacaaggtat ctgtatagcg atcgcccgga caaccctgat ggttggagca tgcatgcttt
     4621 ggactatctt ccaggtgata tgaactctat gtcttccatt actcctgttg aactagcaca
     4681 tggtgatacc ttgtgcacag agtttgccct gttgtttgca ccggcagaag tgccaaatga
     4741 tcctttacat gctttgaata aactttttct gaatgcagag atactgcgtg ccatgtatcc
     4801 atctctttca aacggcgaag agtgtatcgc ctaccgctgg gaaccgtgga acgctccgga
     4861 tgcagatggc atcactttcc ttccctatcc gaatcctgcc agcgatgtgg tctatctgca
     4921 caccgggcct tttcacgaca cagaactgat agtggaactc cgtgatatga ccggtcaact
     4981 ggtggccaga agtacctatg aagggatctt ggaaaaccag acgttatcct ttccactgcc
     5041 cgccctggca agatctcagt atgtattatc aggcctggtt gatggtcacg tattcagtaa
     5101 acagattcaa gtctggtagt aaattttctc cctattttta ccgcatgtcg cacgatgctt
     5161 cggcccgcag ggtcatgcaa tttgaagccg atcaat
//
LOCUS       DAOMAX010000186         5177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2688_length_5177_cov_4.251855, whole genome shotgun sequence.
ACCESSION   DAOMAX010000186 DAOMAX010000000
VERSION     DAOMAX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2688_length_5177_cov_4.251855"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="PK742_09530"
     CDS             complement(<1..1579)
                     /locus_tag="PK742_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQU76869.1"
                     /translation="MKRRIQSVAVLGSGVMGSRIACHFANIGCKVQLLDIVTPGLNET
                     DAANKTKRNQLVNNSLQAAVKQNPSPVYNKQVLSHITTGNFEDDLQSIATADWIIEVV
                     IERLDIKQQLMEKVDAIRKPGTLITSNTSGIPIHRIAEGRSEDFRKHFCGTHFFNPPR
                     YLRLLEIIPTPETDQNVIDFLLDYGDRFLGKQTVLCKDTPAFIANRIGVFSIMTVLQV
                     MDQLQMTVDEVDALTGPVSGRPKSATFRTTDVVGLDTMVKVANNTYEACPDDEARENF
                     RIPAYINTMMENKWLGDKSGQGFFKKVKGDDGKSQILTLDLKTMEYGTQSKPKFATVG
                     EAKQVDDLKQRLVILHNGKDKAGDFYRAITYRVNQYVSNRIPEISDELFRIDDAMRAG
                     FGWELGPFETWDVLGVQQTIEAMKASGFAPAAWVDEMLSAGHTSFYKSENGKRLYYDI
                     PTKTYKEVPGTDAFIILDNYRAQKPVFQNAGATLHDIGDGILNLEFHTKMNVIGAEII
                     EAVNKSIAIAEQNHRGLV"
     gene            complement(1576..2172)
                     /locus_tag="PK742_09535"
     CDS             complement(1576..2172)
                     /locus_tag="PK742_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQU76870.1"
                     /translation="MPVRKVNKEEVVTEALKLFRSRGYHKTTMAEIGAACGLLKGSIY
                     HYFASKEELMIAVLEFLREYYRVNVFAIAYNDKRSAMSRLKELGTHSMDIFIRGEGAC
                     LMGNIASETIDVVPEFHPHIKSFFDDWTDALVYIYRSHYSPKAALFFARETICAIEGA
                     ALMMRIYKDPDFLRKANDMILKKFESATKEALIQQQEQ"
     gene            2282..3226
                     /locus_tag="PK742_09540"
     CDS             2282..3226
                     /locus_tag="PK742_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76871.1"
                     /translation="MFLYPRMLLSLVLGGIAAEIYWLNSSNVLSGWIAAGIGLLCAVV
                     IWLITAKLFPVIHDVATLTSSEDEEERVPDTDCKPEEEVLTGRMAEAKESVSEVNPTV
                     EEESEPAEVYHYDVSTENPEPVTETWKQQALPVGEDSSVVETIIYEAEEEEELPTKAD
                     TTIEVAEDPVVLLMPNELSELGREIESFQVSSYRGGQQIKLYGTEALQVHLQRIAMYY
                     NDTSVFQYTLTKNDEILFGYLTRFYRFAKGCVETSNTCVEVKNFYSRYIEPITEGYLQ
                     LMVQVQNETARDVLKDIYKRARKLRKLMDRPFNKSFAA"
     gene            complement(3227..3787)
                     /locus_tag="PK742_09545"
     CDS             complement(3227..3787)
                     /locus_tag="PK742_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76872.1"
                     /translation="MPFNDFPDEPAPQVLGFGYTLQLGYSAPARYSERLSYRLTYKQF
                     RNPLGGEKFGDPFPIATGMAKSDALLSDLMLTIGEQNRIIAQPFIGAGVGSTRTPSYL
                     SSGNLVTGGDGIMIEEHIGLLVNTRNGQSRIFLEFRMSYLQMYYIEDISTIYELSRSN
                     LISNLVPEFSLVYAIDLKELLQKIQQ"
     gene            complement(4098..>5177)
                     /locus_tag="PK742_09550"
     CDS             complement(4098..>5177)
                     /locus_tag="PK742_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQU76873.1"
                     /translation="ESIFFGSKPTPLSAEKMHLIKVSKTGHVVWQISPDENSICDEIK
                     SLESSGHVMVGDLLYCVCKNQPIVIDMKNLLLQNEYAIDSSTAYQISSFDQLLFYTTK
                     FGSLGTGYDSSVLVVADPFSGRQKTLFRQGKNGDFNPNLYPPEVFVNNPTGDTLLVFQ
                     NRQWDFTNSANGGRVDLFCWNMTADTLVWQIPEIDPYGNSSVCKPLYHDGLVYFRGFQ
                     TLHAVNVETGEQVWMHEFPGDFDDLLLGNMLIAEGKLIVKSSTEGIHAFDPGTGALLW
                     ERYDAGNTSCYMSYFDGSVFYGNYGGELFNVRVADGEILWAYTSKNEGKNLGWEPQFN
                     NGVIFDEENRRIITTDGYYLICLQL"
BASE COUNT         1344 a         1217 c         1226 g         1390 t
ORIGIN      
        1 tcaccagtcc gcggtggttc tgctctgcaa tagcgatgct cttattgacc gcttctatga
       61 tctcagcacc gatcacattc atcttggtat ggaactccag gttcaggatt ccatcaccga
      121 tatcgtgcag cgttgcgcct gcattctgaa agaccggttt ctgtgcccgg tagttatcga
      181 gtatgatgaa ggcatcggta cccggtactt ccttatatgt ctttgttgga atatcgtagt
      241 acaggcgttt cccgttctca cttttataga aggaagtatg ccctgcgctg agcatctcat
      301 ccacccaggc agcaggagcg aaaccagagg ctttcatggc ctctatagtc tgttgtacgc
      361 ccagcacatc ccaggtctcg aagggtccca gctcccagcc gaagcctgca cgcatggcat
      421 catctatgcg gaagagttcg tctgaaattt ccgggatgcg gttagacaca tattgattta
      481 ccctataggt tatcgcccgg tagaaatcgc cggctttatc ttttccgttg tgcaggatga
      541 ccagtcgttg tttcaggtcg tccacctgct ttgcttcacc tactgtggcg aattttggct
      601 ttgactgcgt accatattcc atggtcttca ggtccagagt aaggatctgg cttttaccat
      661 catccccctt cacctttttg aaaaaaccct gaccgctctt atcgcccagc cacttgttct
      721 ccatcatggt gttgatgtaa gccggaatgc ggaaattctc gcgggcctca tcatcggggc
      781 aggcctcgta ggtattgttg gccaccttta ccatggtatc cagtcccacc acatccgtag
      841 tgcggaaggt ggcagatttc ggtcggccgg aaacaggacc tgtcagcgca tccacttcat
      901 ccacggtcat ctgcagttgg tccatcacct ggagtacggt catgatgctg aacaccccga
      961 tgcggttggc gatgaaggcg ggagtgtcct tacacaaaac ggtctgtttg ccgaggaaac
     1021 gatcaccgta atccaggagg aaatcaatta cattctgatc agtttcaggt gtggggatga
     1081 tctctaagag gcgcaggtag cggggcggat tgaaaaagtg ggtaccgcag aaatgttttc
     1141 tgaagtcttc gcttctgcct tcggcgatcc ggtggatggg aataccggat gtattggagg
     1201 tgatgagggt gcccggtttt ctgatggcat ccactttttc catcagttgt tgtttgatgt
     1261 ccaggcgttc gatgaccact tctatgatcc agtcggcggt ggcgatggac tgcaggtcat
     1321 cttcaaagtt tccggtggtg atatgcgaca gcacctgttt gttatagacc ggcgagggat
     1381 tctgtttgac tgcagcctgc aggctgttgt tgaccagctg gttgcgtttg gtcttgttgg
     1441 cggcatctgt ttcgttcagg ccgggcgtga caatatccag cagctgcacc ttgcaaccaa
     1501 tgttggcgaa gtggcaggcg atgcgggatc ccatgacccc ggagcccaga acggctacag
     1561 attgaattct tctcttcatt gctcttgctg ttgtatgagg gcctctttgg tggccgattc
     1621 aaattttttc aggatcatgt catttgcctt gcgcaggaaa tccgggtctt tgtagatgcg
     1681 catcatgagt gcagcacctt caatggcaca gatggtctca cgggcaaaga acagggccgc
     1741 tttgggcgaa tagtgggaac ggtagatata gaccagggca tcggtccagt catcgaagaa
     1801 gcttttgata tgcggatgaa attcaggcac tacgtctatg gtctcggagg cgatattacc
     1861 catgaggcag gcgccttcgc cacggatgaa gatgtccata ctatgtgtgc ccagttcttt
     1921 caggcgtgac atggcagaac gtttatcatt ataagcaatg gcaaacacat tgacccggta
     1981 gtattcacgc aggaattcca gcaccgcgat catcagctct tctttggagg cgaaatagtg
     2041 gtagatgctt cctttgagca gtccgcaggc tgcaccgatc tctgccatgg tggtcttatg
     2101 gtatccacgt gaccggaaga gcttcagagc ttctgtcacc acttcttcct tgttgacttt
     2161 tctaaccggc ataagataaa ggtagagttg ctttttcatt ttaccaaacg tttgtttggt
     2221 aaaataattg tcaactattg cttgacaaac tgatgacaat tcgtttactt ttgtgactcc
     2281 tatgtttctg tatccccgca tgctcctgag ccttgtatta ggcggcattg ctgcagagat
     2341 ctactggctg aacagcagca acgttctgtc agggtggatt gcagccggga tcgggttgtt
     2401 gtgtgccgtg gtcatctggc tgatcacagc gaaacttttt ccggtcattc atgacgtggc
     2461 taccctgacc agctcagaag acgaggaaga gcgtgtacca gatacagact gtaaaccaga
     2521 agaggaggta ctgaccggca gaatggctga agcaaaggaa tccgtttcag aggtcaatcc
     2581 tactgttgaa gaagaatcag aaccagccga agtctatcat tacgatgtaa gcacggaaaa
     2641 cccagagcct gttacagaga cctggaagca gcaggctttg ccggtagggg aagacagctc
     2701 agtagtagag accatcatct atgaggcaga agaagaggaa gagttaccca ccaaggcaga
     2761 tactacaata gaagtagcag aagacccggt ggtgttactc atgcccaatg agctgagcga
     2821 gctagggcgg gagatagaat cttttcaggt aagcagttac cgaggcgggc aacaaattaa
     2881 attatacgga acagaagcct tgcaggttca tttacagcgc attgcaatgt actacaatga
     2941 cacttctgtt tttcagtaca cccttaccaa aaatgacgag atcctgtttg gttatctgac
     3001 ccgtttctac cggtttgcaa aagggtgtgt ggagaccagc aatacctgtg tggaagtcaa
     3061 gaatttctat tcaaggtata tagagccgat cacagagggt taccttcagc tgatggtcca
     3121 ggtgcaaaat gaaacagcca gagatgtact aaaggatata tataaacggg caagaaaatt
     3181 gagaaagctt atggatcgtc cgttcaacaa gtcgttcgcg gcgtgatcac tgttgtatct
     3241 tttgcaataa ttctttcagg tctattgcat aaacgagtga aaattccggt accagattgg
     3301 atattaaatt tgacctgctc aattcataga tggtgctgat atcttctata taatacatct
     3361 gcaaatagga cattctaaac tccagaaaga ttcggctttg tccgtttctt gtattgacca
     3421 ataagccaat gtgctcttct atcattattc cgtctccacc tgttaccagg ttacctgatg
     3481 acagatacga aggtgtcctg gtactgccca ctccggcacc aataaagggt tgtgcaatta
     3541 ttcggttctg ctctccaatg gtaagcatca ggtcactaag aagggcatca gacttagcca
     3601 ttcctgttgc gatcggaaac ggatcaccaa acttttcacc acccagcgga tttctgaact
     3661 gtttataggt aagccggtag gataatcttt ccgaataccg ggcaggcgca gaatagccaa
     3721 gttgcagcgt ataaccgaaa cccagcacct gaggtgctgg ttcatccgga aagtcgttga
     3781 aaggcatgga aagtccccct ccaaaatgaa tggcgggtcc ggggaaaaaa gcattctgag
     3841 cccgagaatt caggcccaga atgacacaaa ccaagggtat aaagtttcac taccaagttt
     3901 taaagaggtg ggttccaccg tggtggaacc cacgcactac acacacactc taccagagtg
     3961 aaagttattc gtcaatggac agaccacgtt gacgacttta aaacatcttt cttgaactga
     4021 gaggatgaaa ctctgccagg ttaccctggc agagttcata ttctcgttct gattacgaga
     4081 gtcttgcggg ttaagcatta cagttgcaaa catatcaaat aatatccatc ggtggtaata
     4141 attctgcgat tttcttcatc aaaaattact ccgttgttga attgaggctc ccagccaagg
     4201 tttttaccct catttttgct tgtataggcc cataatattt ccccatctgc aaccctgacg
     4261 ttaaataatt caccgccata attaccataa aacaccgacc cgtcaaaata actcatataa
     4321 caggaggtgt ttcccgcatc ataacgctcc caaagcaatg ctccggtgcc cggatcaaaa
     4381 gcatgtatgc cttcagtact ggatttgacg atcagttttc cttcggcaat caacatgttc
     4441 cctaacaata aatcatcaaa atctcccgga aactcgtgca tccatacctg ttctccggtt
     4501 tccacattca ctgcatggag ggtctggaaa ccacggaaat agaccagacc atcatggtaa
     4561 agcggcttgc atacactgct gtttccgtag ggatctattt ccggtatctg ccagacaagg
     4621 gtatctgccg tcatattcca gcaaaacaga tccacccggc caccattggc agagttggta
     4681 aaatcccact gcctgttttg aaaaacaagc agggtatctc ctgttggatt atttacaaaa
     4741 acctccggag ggtaaagatt ggggttgaag tcgccgttct taccctgacg aaataatgtc
     4801 ttttgtcgcc cggaaaaagg atcagcaact acaagtacag aactatcata gcctgtacct
     4861 aaactcccga attttgtagt ataaaataat aattgatcaa aactggaaat ctgatatgca
     4921 gtggaagagt ctattgcata ctcattttgc agtaacaagt tcttcatgtc aattacaata
     4981 ggctgattct tacaaacaca ataaagtaaa tcgccaacca taacatgccc agacgactcc
     5041 aaagatttta tctcatcaca aattgaattt tcatccgggc ttatttgcca tactacatgt
     5101 ccagttttgc taaccttaat caagtgcatc ttttctgcac ttaagggagt aggctttgat
     5161 ccaaagaaaa ttgattc
//
LOCUS       DAOMAX010000187         5174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2693_length_5174_cov_3.634499, whole genome shotgun sequence.
ACCESSION   DAOMAX010000187 DAOMAX010000000
VERSION     DAOMAX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_5174_cov_3.634499"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PK742_09555"
     CDS             complement(<1..845)
                     /locus_tag="PK742_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HQU76874.1"
                     /translation="MYYNIQGAENTALGYGTLFENTKGNYNTAIGSISLYDNKLGSYN
                     SALGYLVEVTSNNLSNSAGLGYWAIIDASNKMVFGNSSVSSIGGEVDWTVYSDARIKE
                     QVEENVPGLDFINQLRPVTYHYNITTQNQLMGRMDTAMWTGKYDIEEMAFSGFIAQEV
                     AAAAEDLGYEFSGVDASGRLLGLRYATFVVPLVKAVQELDAENAALQKENEALKARLD
                     RIEQLLNINEETGWKQEATITTTAMLGQNSPNPFTGNTVIPVYVPETATQASLQITDL
                     QGRLL"
     gene            complement(1389..2843)
                     /locus_tag="PK742_09560"
     CDS             complement(1389..2843)
                     /locus_tag="PK742_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76875.1"
                     /translation="MKVNLMLSPPPFYKLIVCLLLTSICGNRMFAQSCDPACEDLSNL
                     TVTTHINAHLLPQPDRNIGSADSSWSDYFMNGIFYIEEQGVGDDLRIMMLNGHRFLHT
                     TGNATGMESPTGLFLGEYAGEDVDATSNGCVGIGYRALEQFVSTDHNTAVGFFAMSAG
                     DAGDKNTMVGNRAGQNIDGGDSNTGIGQNVFYRSGTITNYIEGIGNVGIGATSDNIGV
                     FGNLYDGNYNTAVGFNSVKSLTTGDENTILGYNAGADLTFGDQNIFIGSGESSGDNGT
                     TNGTGNIVIGINEDLHSSSASNRMNIGGVLYADLSANKVSIGNSGSALSNTFNVGAND
                     EFQIDNTGVIKEYNNLTTDGLGVPVILNVLTLSDQAGSITNGMLLEDAPAGVYRVTYQ
                     GVVTVAADVGSALSFKVRYTDADITSGTVDAPSTNITGNNSTTSNTVGSANFSGVVLI
                     QVADASDIKYIVSRASESSGSMEYSLRVLLERIQ"
     gene            complement(2958..4556)
                     /locus_tag="PK742_09565"
     CDS             complement(2958..4556)
                     /locus_tag="PK742_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HQU76876.1"
                     /translation="MKKIVYILIFISSGLVAQPEILWQFDMNDMAFGQAAAADVDGDG
                     LLEVVFSTYRNDGMLYVLNGEDGSVLWQADVGGCADAAPLIADVDMDDDLEIVLAGSC
                     DPTTYCFDADSGFIQWETPMRGSDSPPVIGDIDNDGMPEILHGEFGGYVLCLNGEDGS
                     EVWELEVDTYSWIQTAPTLLDANGDGQLDFVIANWSFGDNHIIQCYSADGPDLLWESD
                     VPDDVIYHGVSWGDMDNNGTIELVIGDYAGILHCFDAATGDTLWNYQFPSNSLYIGAP
                     TSMADLDGDGFLEVVFTDWFQLGAIDRSGELIWDYSIPGYGQAFRGVALADINNDGHP
                     DATFCSTEGKVMAVDGLDGSFIQDIDLEADFGMDFDAEHGPLVADFNNDGTLEVFAAG
                     GHAEYPAIENNYGAAYMISWGEGQGSWTMFQHDPWRTACLCNDSLLQEEEPVAIQQFQ
                     DENSWQVGPSPFSDELFISGPQGETMVRLYDLAGELVWEEKISITAGPNSIVIPASVP
                     LKTGIYFCTLFQNEYYMTRIVLHF"
     gene            complement(4553..>5174)
                     /locus_tag="PK742_09570"
     CDS             complement(4553..>5174)
                     /locus_tag="PK742_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQU76877.1"
                     /translation="RKIADAAHARGIELMIDGAHTFGHFAFDIPSLGGDYYGTSLHKW
                     MSAPIGSGMLWIKKDKISKIWPLMPGDEPQSDNIRKFENLGTRSFPIEQAIGQAVDFH
                     MMIGPERKEARLRYLKDYWTSKVKDLPGVFLNTSLLPAYSCAIANIGVEGREPVDLDN
                     HLFSKYNIHCVTINWENIHGVRIAPHVYTSLRELDRLVEGITSFVA"
BASE COUNT         1306 a         1306 c         1186 g         1376 t
ORIGIN      
        1 tgcagcagcc gaccctgcag atctgtgatc tgcaagcttg cctgtgtggc tgtttccggc
       61 acatataccg gaatgactgt attgccggta aagggattgg ggctgttctg ccccagcatg
      121 gctgtagtgg tgatggtagc ctcctgtttc cagccggtct cttcattgat gttcagcagt
      181 tgttcaatgc ggtccaggcg ggctttgagg gcctcgtttt ctttctgcag agctgcattc
      241 tctgcatcca attcctgcac tgctttgacc aggggcacca caaaggtggc atagcgcagg
      301 cccaggaggc gaccgctggc atccacgccg ctgaactcat accccaggtc ttctgccgca
      361 gcagccactt cttgggcaat gaagccggaa aaggccattt cttcaatgtc gtatttgcct
      421 gtccacatgg cggtatccat gcggcccatc agctggttct gggtagtgat gttgtagtgg
      481 taggtgaccg gccgcagctg gttaatgaaa tccagccccg gcacattctc ttccacctgc
      541 tctttgatgc gggcatcgct gtacaccgtc cagtccacct cgccgccaat gctggagacg
      601 ctggagttgc caaataccat tttattggat gcatcaataa tggcccaata gcccaaaccg
      661 gcactattac tcaggttatt ggaagtaact tctaccaaat accctaatgc tgaattgtaa
      721 gatccaagtt tattatcata gagacttatg cttcctatgg cagtattata gttgcctttt
      781 gtgttctcaa ataaggtgcc atagcctaat gctgtattct cggctccttg aatgttataa
      841 tacattgaat ttgaaccaac tgcggtattg ctgtttgaat ttctattatt gaggagcgct
      901 ccaaaaccaa cagcaacgtt ttgttctccc cctttattgc ctgccaaagc gctcttacca
      961 acagcaacat tgtctgagcc ggtaatgtta aaagacatcg tattatcacc aatagctaca
     1021 ttgttttcgc ctgataggtt aggctttaaa ctttcatatc caactgcaat attaaatcgt
     1081 cctgttatat tatttgcaag tgcattatca cctatagaga tattatcgac agctgttgtt
     1141 atattggaaa gcacatcttt accaaatcct atacctccgg ggcctgagta aatctgcttt
     1201 gtgcccaatt gaagaagcac tggcacctca aagcctaatt gtatgtcaat cggataggca
     1261 ggagaaagcg ttcctatgcc aacgttacca ctaggcggga aggtgttctg tgcctgaatg
     1321 ctttgagcaa taataaaaat tattacaaat tgtcctagta tttttttcat gacgtttaat
     1381 tttatttatt attgaatacg ttctaaaagt acccggagag aatattccat gctgccagaa
     1441 ctttcactcg ctctgcttac tatgtatttg atatcagaag catcagctac ctgaataagg
     1501 actacgccac tgaaattagc agaaccaacc gtgttcgaag tagttgaatt attgcctgtg
     1561 atattggtag aaggagcatc tacggttcct gaagttatat cggcatcggt ataacgtacc
     1621 ttaaaggaaa gcgcagagcc tacatctgca gccacggtaa caactccctg gtaagtaacc
     1681 cgataaaccc cggcaggagc atcctccagc agcatgccat tggtgatgga acctgcctgg
     1741 tctgacaagg taagcacatt cagtataaca ggaacaccca gcccatctgt tgttaggttg
     1801 ttatattctt taattacacc cgtattgtct atttgaaatt cgtcattggc acctacattg
     1861 aaggtgttag acaaagcaga accactattt cctatactga ctttattggc gcttaaatct
     1921 gcataaagaa caccgcctat attcatgcgg ttgctcgctg aagaactatg caagtcttcg
     1981 ttgatcccta taactatatt gcctgttccg ttggttgttc cattgtcacc cgatgattca
     2041 cctgaaccaa taaaaatgtt ttgatcacca aaagtaagat ccgcccctgc attgtaccct
     2101 agtatcgtat tttcatcacc tgttgttaga ctctttaccg aattaaaacc tacggctgta
     2161 ttataattcc catcatacag attgccaaaa acgcctatat tatcactggt tgcccctatg
     2221 cctacgttcc ctataccttc aatgtaatta gtgatagtac cacttctata aaatacattt
     2281 tggcctatcc ctgtgttaga atcaccacca tcaatgtttt gtccggcccg gttacccacc
     2341 atcgtattct tatcaccggc atctcctgcg ctcatcgcaa aaaagccaac agctgtattg
     2401 tggtcagttg acacaaattg ttccaaggct ctatagccta tgcctacaca accattgctg
     2461 gtggcatcaa catcttcgcc ggcatattca cctaaaaata gaccggttgg gctttccata
     2521 cccgtagcat tgccggtagt gtgtaaaaac ctatggccat tgagcatcat gatgcgcaga
     2581 tcatcgccca caccttgttc ttctatataa aaaatgccgt tcataaaata gtcagaccag
     2641 cttgagtctg cagaacctat atttctgtca ggctgcggca ataaatgcgc attaatatgg
     2701 gtggtaacag tcaagttact caggtcttca caggcgggat cacatgactg tgcaaacatg
     2761 cggttaccac aaatggatgt gagcaacaaa caaacgatca gtttgtaaaa tggggggggg
     2821 cttaacatca aattaacctt catggtgttg cgtttttgag gtgaatcaat gaaggcagaa
     2881 ggggggcatg gtctaagtta acgataaaag atcggataat ctgaaaaaac gtctgacagt
     2941 ttcagtaaac aaggctgcta gaagtgcaaa acaattctgg tcatataata ctcattttga
     3001 aagagtgtgc agaaatatat acctgtcttc agcgggacag atgcaggaat aacaatgcta
     3061 ttgggtcctg ccgtaatcga tatcttttcc tcccaaacca attccccagc cagatcatac
     3121 aacctgacca tggtctctcc ctgtggtcct gaaatgaata gctcatcact gaacggtgag
     3181 ggaccgacct gccagctgtt ttcgtcctgg aattgttgga tagctacggg ctcttcttcc
     3241 tgtaacaggc tgtcgttgca aagacaggct gtgcgccagg ggtcgtgctg gaacatggtc
     3301 caggaaccct gtccttcgcc ccagctgatc atatatgctg cgccgtaatt gttttcgatc
     3361 gcaggatatt ctgcatgtcc gccggcggcg aagacctcca gtgttccgtc gttgttgaag
     3421 tcggccacca gcggaccgtg ctctgcgtcg aagtccatcc cgaagtcggc ttcgaggtcg
     3481 atgtcttgaa tgaaagaacc atccaggccg tccactgcca tgaccttgcc ttcggtggag
     3541 cagaaggtag catcgggatg gccatcgttg ttgatatcgg ccagggccac cccgcgaaag
     3601 gcttgtccgt atccgggaat gctgtagtcc caaatgagtt caccagatcg gtctatggct
     3661 cccagctgaa accagtcggt aaataccact tccagaaaac catcgccatc cagatcggcc
     3721 atgctggtag gggcgccgat gtagagtgag ttggagggga actggtagtt ccaaagcgta
     3781 tcgccggtgg cggcatcgaa gcaatggagg atgccggcat aatcgccgat gaccagttca
     3841 atcgttccgt tgttatccat atcgccccag gagacgccgt ggtagatgac atcatcgggc
     3901 acatcagatt cccagagcag gtctggacca tcagccgaat agcattgtat gatgtggttg
     3961 tcgccaaagc tccagttcgc aatgacaaag tccagctggc catcgccgtt ggcatccagc
     4021 agggtaggag cggtttgtat ccaggaatag gtatccactt ccagctccca aacctcagaa
     4081 ccgtcttcgc cgttcaggca gagcacataa ccaccgaatt cgccgtgcag gatctcgggc
     4141 ataccatcgt tgtcgatatc accgatcaca ggaggcgagt cgcttccccg cataggtgtt
     4201 tcccactgga tgaagccact gtcggcatcg aagcaatagg tagtaggatc acagctgccg
     4261 gccagcacga tctcgaggtc atcgtccatg tccacatcgg caatgagggg agcagcatcg
     4321 gcgcagccac ccacatcggc ctgccagagc acagaaccat cttcaccgtt cagcacatac
     4381 aacataccat cgttccggta cgtggagaac actacttcca gcagtccatc accatccaca
     4441 tctgcagcag cagcctggcc aaaggccatg tcgttcatat cgaactgcca gaggatctcc
     4501 ggttgtgcaa ccagaccgga tgatatgaat ataagaatat atactatttt tttcatgcta
     4561 caaagcttgt gatgccttcc accagtcggt ccagttcacg cagggaggtg tacacatgcg
     4621 gagcaatgcg aaccccatga atattctccc agttgatggt cacgcaatgg atgttgtact
     4681 tgctaaacag gtggttgtcc agatccacag gttcacggcc ttccacaccg atgttggcta
     4741 tggcgcagga gtaagcaggc aggagactgg tattcagaaa aacacccggt aggtctttca
     4801 cttttgaggt ccagtagtct ttcaggtagc gcagacgtgc ttcctttctt tccggtccga
     4861 tcatcatatg aaaatcaact gcctggccga tggcttgttc gatagggaag gagcgggtgc
     4921 cgaggttctc gaatttcctg atgttgtcac tctgcggttc atcgcccggc atgagcggcc
     4981 agatcttgct gatcttgtcc tttttgatcc agagcatacc gctgcctatc ggtgcactca
     5041 tccatttgtg caatgaagtg ccgtaatagt cgccacccag cgatggaata tcaaaagcga
     5101 aatgtccaaa agtatgtgct ccgtctatca tcagttcgat gcctctggca tgtgccgcat
     5161 cggcgatctt tcgc
//
LOCUS       DAOMAX010000188         5146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2716_length_5146_cov_3.514830, whole genome shotgun sequence.
ACCESSION   DAOMAX010000188 DAOMAX010000000
VERSION     DAOMAX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_5146_cov_3.514830"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PK742_09575"
     CDS             complement(<1..1376)
                     /locus_tag="PK742_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HQU76878.1"
                     /translation="MIISRTLSSSDWQINMPASGRKDLRWHKTGRYTYIIYGYPVHLQ
                     NAAPFDPEHIIQADLDSPAHYFDRTDGHGTLICLDHHRQELTIYTDPYRLYVLYFYLT
                     AEKMIISDSLYEIRREVKLTVNEQAVIELSVINTTIGNKTLYQEIKTPDEGCCYTLRV
                     PGQEPAVSVHRPFPPPLSGSLDREAFIQRFRSWTAGGIALSQGTSLALTAGLDSRVSL
                     ASSLEDPSRIHTYTHGFSTSMDVRVAARLAGRAGVKHRFFDLDNENFIREIPAFFREV
                     NSSYEGSLNALSHAHAVNSYHRQMEVADTFLSSFGGELLRSYYLPPGLKRGASLDAMA
                     DGIRKISSVPTDLSLFNDPATVEDRLNQAIYEELKQAPETADLSYLADYYYHRRNFNS
                     VTTRFAGRYFRVFSPYFSRCVYDASLLADPEVRRSGDLQKWLVQPAGKKLTSLLLNDK
                     EPVEESVRL"
     gene            1459..2295
                     /locus_tag="PK742_09580"
     CDS             1459..2295
                     /locus_tag="PK742_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76879.1"
                     /translation="MQKKLFAAVCITALLTTSIAVHAQAPVDGFYPGKGNGAVALSYS
                     HEKYDQFFLGNGSIANWAGDYNVNSVSLYATYGITDKFAAAVSVPYIMIKTVNFSDET
                     VKRKSLQDLGVYLKYRALEKDFGSWNLSLSPAIGFTTPITDYATDFYAVGQDATGLDI
                     RGIAMVTTDMGVFGEVQGAGLLRFNPTPSGASFNVKAGYYNAKIYGDVYYSIQQINGG
                     VGLPDPEDFRELAVSYQKVGVTVAYNILSSLGVYAGGAYVIDGKSVGQSTRITGGAVV
                     RF"
     gene            complement(2298..2738)
                     /locus_tag="PK742_09585"
     CDS             complement(2298..2738)
                     /locus_tag="PK742_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76880.1"
                     /translation="MKQEMTRRMMIIFYSIMAGHILFACVIVFFVKPVPAENSLNLKN
                     LTSILAAASVAVTMLAYWLYQQQVTKIASSDNDDIDKLNAWQAPVIIRLAMIEGVCLA
                     NLVALYLTGYSLYLYFYVATALAMLYAKPRPLSIATELQLQEDQ"
     gene            complement(2735..3568)
                     /locus_tag="PK742_09590"
     CDS             complement(2735..3568)
                     /locus_tag="PK742_09590"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HQU76881.1"
                     /translation="MGNRHHTWFRTWKGKNLFMQEWMIDRPTAVVGVIHGQSDHSSRF
                     THVAEALNNAGYGVAAIDLIGHGRSEGKRGHVSSFHDYISQVGLLHEHLKSSGLPVFL
                     YGQSMGGLIVLSYAQHHPENVAGVISSSPWLKLAFEPPAWRVALGKVVLRIAPGLVQP
                     AELDLSALAHPEQVQLAYKNDPLVHGKISPAAYFGIVEAGEKWRREWPVLKMPVLLLH
                     GNADRITDHTSSASYADTKPANLTYREFAGLYHELHNEVEQEEVFTTVLDWLQQQISN
                     K"
     gene            3610..4671
                     /locus_tag="PK742_09595"
     CDS             3610..4671
                     /locus_tag="PK742_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQU76882.1"
                     /translation="MFKKVLRTIIVLAVLAILYFGGIILYGTITEYKPVPGSTELLSV
                     SGQPAGTTPDSVIRILNWNLGFGGLGAEMDFFYDGGHMVRAERSVWDKDFVGQLQTLK
                     DNQPIDFYFLQEADIHSKRSYYVNMVDSIGRMLQEYSWTFGMNYYVQHVPLPITRPLG
                     HIESGVVIYSRYPVLQPERHQYPGSFPWPTRIFFLDRCYLVNRIATKNNKELVLINTH
                     NSAYDETGRIKEEEMQVLKDYCEKEYAKGNYVVVGGDFNQCPPGFDPHTWDPGDYPLF
                     IPPAMDPDMLPAGWTVAFDPSHPTNRHLDKPYADDSFKTLIDYFVLSPNISVNNIHTI
                     DNQFRFTDHQPQLLELSLH"
     gene            4673..>5146
                     /locus_tag="PK742_09600"
     CDS             4673..>5146
                     /locus_tag="PK742_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQU76883.1"
                     /translation="MPYVLAIDAGTTSCRTLLFDEKLQVAGLAQEEFRQYFPQPGWVE
                     HDAEEIFETQYRTLLKVLDKTSVPLEEIVAAGITNQRETVVVWNRHSGKPIYPAIVWQ
                     DKRTESVCKHLRQEHPGIRQKTGLVVDAYFSATKINWILEHVPGARDAAESGDLLC"
BASE COUNT         1365 a         1237 c         1197 g         1347 t
ORIGIN      
        1 aggcgaacac tttcttcaac cggttcctta tcattgagaa gcagtgaggt gagtttttta
       61 ccggcaggct gcaccagcca tttctgcaga tctcccgatc tcctgacctc agggtctgca
      121 agcagggaag catcatacac acatctggaa aagtacgggc taaagaccct gaaatacctt
      181 ccggcaaaac gtgtggttac actattgaaa tttcttcggt ggtagtaata gtcagcaagg
      241 taagagagat cagctgtttc aggcgcctgc ttcagctctt catagatggc ctgattgagg
      301 cggtcttcca ccgttgcagg gtcattgaac aaagacaagt ctgtcggaac tgaagatatt
      361 ttccttattc catctgccat cgcatccaga gaagcacctc ttttcagccc gggaggcaga
      421 tagtaagatc gcagcaactc tccgccaaaa gaagaaagaa aggtgtctgc cacttccatc
      481 tgtctgtgat agctattgac agcatgtgca tgagacaaag cattcaggct gccttcatag
      541 gatgaattga cttctctgaa aaatgccggt atctcacgaa tgaagttttc attgtccagg
      601 tcgaagaaac gatgcttcac tcctgccctt ccggccagcc tggcagccac ccgtacatcc
      661 atgctggtgc tgaacccatg tgtataggta tgaatgcgtg aagggtcttc cagagatgat
      721 gccagtgaaa ccctgctgtc cagccctgcg gtaagtgcca gactggtgcc ctggctgaga
      781 gcaatgccac cggccgtcca tgaacgaaag cgttgaatga atgcctcccg gtctaaggaa
      841 cctgatagcg gaggcggaaa aggtctgtgt actgaaacag ccggttcctg ccccggaaca
      901 cgcaatgtgt aacaacaacc ttcgtcagga gtcttgatct cctgatacaa ggttttgttg
      961 ccgatggtgg tgttgatgac cgaaagctca atcaccgcct gctcattgac ggtcagcttc
     1021 acttcccgtc tgatctcata taacgaatcg ctgatgatca ttttttcagc agtcaggtag
     1081 aaatacaata cgtacaacct gtaaggatca gtatatatgg tcagttcctg cctgtggtga
     1141 tccaggcata tcagtgtacc atgaccgtca gtcctgtcaa agtaatgtgc cgggctgtcc
     1201 agatctgcct gtatgatatg ctcgggatca aacggggcgg cattctgcag atgcaccggg
     1261 taaccataaa taatataggt gtacctgcct gttttgtgcc accgaaggtc ctttctgcct
     1321 gatgctggca tattgatctg ccaatctgaa ctgctaaggg tacggctgat gatcatattt
     1381 ttttaccgaa cttttaaact tctctccttg tataacctac ttatccaagt atttttgaaa
     1441 atccaatcaa accatactat gcagaaaaaa ttatttgcag ctgtgtgcat cacagcattg
     1501 ttgacaacca gtatcgcagt gcatgcacag gctcctgttg atggctttta tcccggtaaa
     1561 ggtaatggtg cggtagcgct ttcttattcc catgaaaagt atgatcagtt ttttctggga
     1621 aacgggagta ttgctaactg ggcaggcgat tataatgtga attccgtgag tttgtatgcc
     1681 acatatggaa tcacagataa gtttgctgct gcagtttctg ttccctacat catgatcaaa
     1741 acggtcaact tcagcgatga aacagttaag cgcaaatcat tacaggatct tggagtctat
     1801 ctgaaatacc gggcattaga aaaggatttt ggcagctgga atctttcgct ttctcctgct
     1861 attggattca ccacacctat taccgattat gccacagact tctatgctgt ggggcaggat
     1921 gctaccggcc tggacattcg tggtattgct atggtcacta cagacatggg agtttttggt
     1981 gaagtgcagg gagcaggact tttgcgcttc aaccctaccc caagcggagc aagtttcaac
     2041 gttaaagctg gttattacaa tgcaaagatc tatggcgatg tctattacag cattcagcag
     2101 atcaacggcg gagtaggcct tcctgatccg gaagatttca gagaactggc agtcagttat
     2161 caaaaggttg gtgtcactgt tgcctataat atcctctctt cccttggagt atatgcaggt
     2221 ggcgcctatg tgatagatgg aaaaagtgtt ggacagtcca cccggatcac tggtggcgca
     2281 gtagtacgct tttaatatca ttgatcttcc tgcaactgca attcagttgc aatggacaac
     2341 ggccttggtt ttgcatacag catagccagg gccgttgcca catagaagta cagatacagg
     2401 ctgtacccgg tcaggtacaa ggcaaccagg ttggccagac aaactccttc gatcatagcc
     2461 aggcgtatga tgaccggtgc ttgccaggca tttagcttat caatatcatc attatctgag
     2521 gaagcgatct ttgttacctg ctgctgatac aaccaatatg ccagcatagt gactgcaacc
     2581 gatgcagcag ccaggatgct ggtgagattt tttaggttca gactgttctc agcaggaact
     2641 ggtttgacga agaatacaat cacacaggca aacagaatat gtccagccat gatgctgtaa
     2701 aagatgatca tcattcttcg ggtcatttcc tgtttcattt gtttgaaatt tgctgctgaa
     2761 gccagtccag cacggttgta aatacttcct cctgctcaac ttcgttatgt agttcatggt
     2821 acagccctgc aaactctctg tacgtcaggt ttgcgggttt agtatctgca taggatgcag
     2881 atgaagtgtg gtctgtgatc ctgtcagcat tgccatgtaa gagcaggaca ggcattttta
     2941 aaaccggcca ttcccttctc catttttcgc cggcttctac aattccgaaa taagcggcag
     3001 gagagatctt tccatgcacc aacgggtcat tcttataagc caactgtacc tgttcagggt
     3061 gcgccagcgc tgaaagatcc agttcggcag gctgcaccag gcccggtgcg attcgaagta
     3121 ctactttacc cagtgctacc cgccaggccg gaggctcaaa ggcaagtttt aaccagggcg
     3181 aagaagagat cacacctgca acattctcag gatgatgttg tgcataagac aatactataa
     3241 gacctcccat actttgaccg tacaaaaaaa ccgggaggcc cgaagatttc agatgttcat
     3301 gcagtaaccc aacctgcgaa atataatcgt ggaagctgct tacatgtcca cgtttcccct
     3361 ctgaccttcc atgcccgata aggtcaatgg cagctactcc ataacctgca ttgttcaatg
     3421 cttctgctac atgcgtaaat cttgatgaat gatcactttg tccatgtatg acaccgacta
     3481 cagccgttgg cctgtcgatc atccattcct gcatgaacag attcttacct ttccatgtgc
     3541 ggaaccaggt gtggtgtcgg tttcccataa ttcggactaa atttaaggca caaagtcaac
     3601 ctttccgcta tgttcaaaaa agtactccga accattatcg tactggcagt gctggccatc
     3661 ctgtattttg gtggtatcat tctgtatgga acaataacag agtacaaacc agtgcccgga
     3721 tcaacagaac tgctttctgt gtccggacaa cctgccggca ctacgccaga ttccgtcatc
     3781 aggatactga actggaatct ggggttcggc ggtctgggag cagaaatgga cttcttctat
     3841 gatggcggac atatggtacg ggcagaacgg tctgtctggg acaaggattt cgttggtcaa
     3901 ctgcaaacac tgaaagacaa ccagccaata gatttttatt ttctgcagga agcagatata
     3961 cattccaagc gcagttacta cgtcaacatg gtagattcca tcggcagaat gttacaggaa
     4021 tattcctgga ccttcggcat gaactactat gtacaacacg taccacttcc cattaccagg
     4081 ccactgggtc atatagaaag cggcgtggtc atttattcac gttatccggt gctgcaacct
     4141 gagcggcatc agtatcccgg ctcattcccc tggcctacac gcatcttttt cctggaccgc
     4201 tgctatctgg taaatcgcat tgccaccaaa aacaataagg aacttgtact gatcaatacc
     4261 cacaactcag catatgatga aaccggccgc atcaaggagg aagaaatgca ggtattaaaa
     4321 gactattgcg agaaagagta tgccaaagga aactatgtgg tagttggagg cgatttcaat
     4381 caatgccctc cgggattcga cccgcacacc tgggaccccg gcgattatcc gttgttcata
     4441 cctccggcca tggatcctga tatgctgcct gcaggctgga ccgtggcatt tgacccatca
     4501 catcccacca acaggcatct ggacaaacca tacgctgatg acagcttcaa aacacttatt
     4561 gattattttg ttttgtcgcc caatatttct gtcaacaaca tacataccat agacaatcaa
     4621 ttcaggttta cagaccatca accacaacta cttgaactaa gtcttcactg atatgcctta
     4681 tgtccttgct attgatgccg gtactaccag ttgcagaacg ctgctctttg atgaaaaact
     4741 acaggtagca ggtcttgccc aggaggaatt ccggcaatat tttccacagc cgggatgggt
     4801 ggagcatgac gcagaagaga tctttgaaac gcagtaccgt acactcctga aagtgctgga
     4861 taaaacatcc gttcctcttg aagagatcgt tgctgcaggc atcacgaatc aaagagaaac
     4921 tgtagtggta tggaacaggc atagcggtaa gcccatttac ccggctattg tctggcagga
     4981 taagagaaca gaatctgtct gtaaacatct caggcaagag catcccggca tcaggcaaaa
     5041 aacggggctt gtagtagatg cctacttcag cgctaccaag atcaactgga tactggaaca
     5101 tgttccgggt gccagagatg ccgcagaaag tggtgatctg ctttgt
//
LOCUS       DAOMAX010000189         5127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2742_length_5127_cov_4.477524, whole genome shotgun sequence.
ACCESSION   DAOMAX010000189 DAOMAX010000000
VERSION     DAOMAX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2742_length_5127_cov_4.477524"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PK742_09605"
     CDS             <1..1730
                     /locus_tag="PK742_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQU76884.1"
                     /translation="NILKPALARGELRSIGATTLNEYQKYFEKDKALERRFQKVMVDE
                     PSVEDAVSILRGLKERYENHHKVRIKDEAIIASVELSYRYISDRMLPDKAIDLMDEAA
                     AKLRLEMDSVPEELDEIERRIRQLEIEREAIKRENDEKKLEDLGKEIADLSEDRNALK
                     ARWQEEKGLVDQLQAKKVDIEQFKLQAEQAEREGDYGRVAELRYGKIKEAEKSIEELQ
                     KQIEDMDSSHRLMKEEVDAEDIADIVSRWTGIPVSKMMESEKMKLLRLEDELHKRVVG
                     QDEAIVAVADAIRRSRAGLADAKKPIGSFIFLGTTGVGKTELAKAISEFLFDDEQAMT
                     RIDMSEYQERHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKAHPDVFN
                     ILLQVLDDGRLTDNKGRTVDFRNTIIIMTSNIGSHIIQEKFEGVTEENLLAATEVARL
                     EVMELLRRTIRPEFLNRIDEIIMFKPLMRSEIRGIVSIQLGLLQGHLDELGVHMRFTE
                     HLVDWLAAEGFDPQFGARPLKRVIQKEIVNTLSKRILAGEVHKEQMLVADVVKDEVVF
                     FNEADEIAA"
     gene            1792..2463
                     /gene="pdeM"
                     /locus_tag="PK742_09610"
     CDS             1792..2463
                     /gene="pdeM"
                     /locus_tag="PK742_09610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HQU76885.1"
                     /translation="MDLHLLNQHWTLLPEKAILWKNASTLLLSDLHLAKDSHFRKHGF
                     AVPPQMSGQTLSRLSTLLDTLKPERLLLLGDLFHSEANSGLDLFSKWRQDFRSLSMVL
                     VAGNHDILDRQWYHDQHLEVHDVLQEGPFTFQHEPQEDAASYQVCGHLHPAVRISGNA
                     RQSLRFPCFWIGQHQTVLPSFGEFTGSKTIRFKKADRVVAIAEGELFLVNGGLNSAKG
                     LPGSE"
     gene            complement(2429..2995)
                     /locus_tag="PK742_09615"
     CDS             complement(2429..2995)
                     /locus_tag="PK742_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQU76886.1"
                     /translation="MTETAIIPAPGLILLSEPFMADPHFKRTVVLLCEHSDDDGTVGF
                     VLNRPLGMKVCDTLVDMDMVDSELFYGGPVAQDTMHYLHTYGSAVEDSLHIMDNIYWG
                     GNFEQITEQLNLGILDPKQIKFFLGYSGWSPGQLGDELKENSWLTASGKGQYVFGIPE
                     ASLWSKILQDKGGRYAEVIHYPEDPSLN"
     gene            3124..5049
                     /locus_tag="PK742_09620"
     CDS             3124..5049
                     /locus_tag="PK742_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76887.1"
                     /translation="MRLLSTLAIAVAVCCHGTVQAQLELTALGVAATEDFSGFAGAGF
                     DASPDDGMLDSDTWIVTGLSDGDVDFGGSATTGDFARGSTMGGITTGGVYAADILDNQ
                     ALMVQPGADDFTPGAFALQMINNTGATIGELEVFYAIYYLNDQGRSNSFNLSYSYDHE
                     TWVDLSDFDFISPEAATFLPDSVNRSGTITGLTWNDGEFFYLRWHGDDVAGSGSRDEF
                     ALDDVSVTGFEGEAIPVIGFSGTSATVSEDAGSLDISVNVSTDSDCELTVELDGSSTA
                     TEGVDFTFSSPVGITFSTGGALSELLNIPITNDTDIEGDETIVLTLTDPTGTCVLGVA
                     DMITITIADDDEPVATEADIADVTGEDADGIATSDGELVTITGLVYGYNLRSGGLEFT
                     LIDETAGIKVFSFSETFGYTPNEGDELTMTGTITQFNGLTEIEPESLEVLSTGNTLKV
                     PATVSALDESTESDLIHLTGCTLVDDAEWLGDGSSFNVNITCDGSGTLVMRIDDLVDL
                     STATVPDGAFNLTGIGSQFDTDAPYLDGYQIMPRYTADIDMIEDTAIAIVDLDPVMIE
                     LYPNPVSRLVHVHAPGIQMDMVTISDLQGQVMMQEMVSRTGQSAVTLELSALPSGMYL
                     LHIISGDRLLTTTLVKE"
BASE COUNT         1334 a         1189 c         1371 g         1233 t
ORIGIN      
        1 ccaatatcct caaaccggca ttggcaaggg gagaactgcg cagtattggc gccaccaccc
       61 tgaacgaata ccagaaatat tttgaaaaag ataaagccct ggagaggcgt ttccagaagg
      121 ttatggtgga tgaaccatct gtagaggatg cggtgagtat tctacgcgga cttaaagaac
      181 gctatgaaaa ccaccacaag gtgcgcatca aggatgaggc catcatcgca tctgttgagt
      241 tgagttaccg atatatctca gaccgtatgc tgcccgataa ggccatagac ctgatggacg
      301 aagctgctgc caaactccgg ctggaaatgg actctgtacc ggaagagctg gatgaaatag
      361 aacgtcgcat tcgccagctg gaaatagaac gggaggccat caaacgggag aacgatgaaa
      421 agaaactgga agacctgggt aaggagatcg ctgacctctc tgaagaccgc aatgcgctga
      481 aagcccggtg gcaggaagaa aaaggtttgg ttgatcagct gcaggcaaaa aaggtggaca
      541 ttgaacagtt caaactgcag gccgagcagg cagaacggga aggtgattac ggccgggtgg
      601 cagaactgcg ttatggcaag ataaaggaag cagaaaagtc cattgaagaa ttgcagaaac
      661 agatcgaaga tatggacagc agccaccgcc tgatgaaaga ggaagtggat gccgaagaca
      721 ttgctgatat tgtttcgcgg tggaccggta ttcctgtcag taaaatgatg gaaagtgaga
      781 agatgaaact actgcggctg gaagacgaac tccacaaaag ggtggtaggg caagatgaag
      841 ccatcgtggc tgtagcggat gccattcgac gctcacgtgc cggactggca gatgcgaaga
      901 agcccattgg ctccttcatt ttcctgggaa ccacaggtgt gggaaaaaca gaactggcaa
      961 aggccatcag cgaatttctg tttgatgatg aacaggccat gacccgcatt gacatgagtg
     1021 aataccagga gcgccacagt gtgagcaggc ttattggtgc gcctccgggt tatgtgggct
     1081 atgatgaagg cggacagctg accgaagccg tgcgcaggaa accatatagt gtggtgctgc
     1141 tggatgagat cgaaaaagca cacccggatg tattcaacat attgctgcag gtgctggatg
     1201 acgggcggct tactgacaac aaaggtcgca cggtcgattt ccggaatacc atcatcatca
     1261 tgaccagcaa catcggttca cacatcatcc aggagaaatt cgaaggggtg acagaagaga
     1321 acctgctggc tgcaaccgag gtggcaagac tggaggtcat ggaattgctg cgcagaacca
     1381 tccgtccgga attcctgaac aggatcgatg agatcatcat gttcaagccg ctcatgcgct
     1441 cagagatcag gggcatcgta tccatacagc tcgggctgtt gcaaggtcat cttgatgagt
     1501 tgggggtaca catgcggttt accgaacacc tggttgactg gctggcagca gaaggatttg
     1561 atccgcaatt cggagccaga cctttaaagc gagtgatcca gaaagagatc gtgaacacct
     1621 tatccaaacg gatactggct ggtgaggtgc ataaggagca aatgctggtg gcagacgtgg
     1681 taaaagatga ggtggtcttc ttcaatgaag ccgatgagat agctgcctga acagggtaag
     1741 caagatcata gaatagaagc aataaaattt ttgtcttcta tttttggcca tgtggatctg
     1801 catcttctga atcagcattg gaccctgctc cctgaaaagg ccatcctgtg gaagaacgct
     1861 tcaactttgt tgctgagtga tctgcacctg gcaaaggaca gtcatttcag aaagcatggg
     1921 tttgccgtgc caccacaaat gagtggccag actcttagca gactgagcac tttgcttgat
     1981 acactgaaac ctgagcgatt gctgttgctg ggcgatctgt ttcacagcga ggccaacagc
     2041 gggctcgacc tgttcagcaa atggagacag gatttcagat cactttccat ggtcctggtg
     2101 gcaggcaacc acgacattct ggacaggcag tggtaccatg atcagcacct ggaagtacat
     2161 gatgtactcc aggaaggtcc gttcaccttt cagcatgagc cgcaggagga tgcagcttcg
     2221 tatcaggttt gcggccatct gcatcctgcg gttcgcattt caggaaacgc caggcaaagc
     2281 ctgcgctttc cctgtttctg gatcgggcaa caccaaactg ttcttccttc ctttggtgag
     2341 tttaccggca gcaagaccat ccggtttaaa aaagcagaca gggtagtggc tatagcagag
     2401 ggagagctgt ttctggtaaa tggaggcctt aattcagcga agggtcttcc gggtagtgaa
     2461 tgacctctgc atacctgccg cctttgtctt gcaggatctt actccacagt gatgcttccg
     2521 gaataccaaa tacatactgt ccctttccgg aagccgtaag ccagctgttt tctttcagct
     2581 catcacccag ttgccccggg ctccagccgc tgtaacccag aaagaatttg atctgctttg
     2641 gatccagtat tcccaggttc agctgttctg tgatctgctc gaaattgcca ccccagtata
     2701 tgttgtccat gatatgaaga ctgtcttcca ctgcagatcc gtaggtatgc aggtagtgca
     2761 tggtatcctg tgctacgggg ccaccataga ataattcact gtcaaccatg tccatgtcaa
     2821 ccagcgtatc gcataccttc atgccaagtg gtctgttcag cacaaaaccc acagttccgt
     2881 cgtcatcaga atgttcacac aacaacacca ccgtgcgctt gaagtgcgga tcagccatga
     2941 acggttcaga caagaggatc agtcccggag cgggtataat ggcagtttct gtcatagaac
     3001 gaaagtaaga tttcctgaaa aatggggtac atcttttcca ccagatatta ccggaatgtg
     3061 aattcaggtc tggtggtttg tgccaccacc cgctctggcc tacctttaca caaaatattg
     3121 aacatgcgat tactttctac ccttgccatt gctgtagctg tttgctgcca tggcacggta
     3181 caggcacagc tggagcttac ggctctgggt gttgcggcta ctgaagactt ttccggattt
     3241 gccggtgcgg gttttgatgc ttcaccggat gatggcatgc tggactctga tacctggatc
     3301 gtgaccggat tgagtgatgg cgatgtggac tttggaggct ctgctaccac cggcgatttt
     3361 gccaggggaa gtaccatggg cggcatcact acaggtggtg tctatgcagc agatatcctg
     3421 gataatcagg cattgatggt gcagccgggt gcggatgatt ttacccccgg agcatttgct
     3481 ctgcagatga tcaacaatac cggagccacc atcggcgaac tggaagtgtt ctacgccatt
     3541 tattacctga acgatcaggg cagatcaaat tcatttaacc tgtcatattc ctatgaccat
     3601 gaaacctggg tagatctgtc tgattttgat tttatatcgc cggaggcagc taccttcctg
     3661 cctgactctg taaaccgttc cggtaccatt accggcctga cctggaatga cggagagttt
     3721 ttctacctgc gctggcatgg agatgatgtg gcaggatcag gttctcgtga tgaatttgcc
     3781 ctggatgatg tatctgttac tggatttgaa ggagaagcca tacctgtgat aggtttttca
     3841 ggaacttccg ccactgtcag tgaagatgca ggatctctgg atataagcgt gaatgtgtca
     3901 accgacagtg attgtgaact caccgttgaa ctggatggca gctctactgc caccgaagga
     3961 gttgacttta ccttcagctc tcctgttggt attacctttt ctaccggtgg agcacttagt
     4021 gaactgttga atattcctat caccaatgat acagatattg aaggcgatga gaccattgtt
     4081 cttaccctga cagatcctac cggaacctgc gttcttggcg ttgctgatat gatcaccatt
     4141 actattgctg atgacgatga accggtggct actgaagcag atattgctga tgtgacggga
     4201 gaagatgctg acggtattgc tacatctgac ggcgaactgg taaccattac cggtctggtg
     4261 tatgggtaca acctgcgcag cggcggtttg gaattcaccc tgatagatga aactgccggc
     4321 atcaaagtat tcagcttctc agaaactttt ggctataccc cgaatgaagg agacgagctg
     4381 accatgaccg gcaccatcac ccagttcaac ggacttaccg agatcgaacc cgaaagcctg
     4441 gaagtgctga gtacaggcaa cacactgaaa gttccggcta ccgtttcagc actggatgaa
     4501 agtactgaat ctgacctgat ccacctgaca ggctgcacac ttgttgatga tgcagaatgg
     4561 ctgggtgatg gatccagttt taatgtcaat atcacctgcg atggttccgg cactctggtc
     4621 atgcgcatcg atgacctggt ggacctgtca acagcaacag ttccggacgg agctttcaac
     4681 ctgaccggca tcggttcaca gtttgacaca gatgcacctt atctggatgg ctaccagatc
     4741 atgccacgct acacagcaga tatagacatg atcgaggata ctgctatagc catagttgat
     4801 ctggatccgg ttatgatcga gctgtatcct aatccggtaa gcaggctggt gcatgtgcat
     4861 gcgccaggaa tacaaatgga catggttacc atttcagatc tgcagggtca ggttatgatg
     4921 caggagatgg tcagcagaac aggacagtct gccgttactc tggaattgag tgcgctgcca
     4981 tcaggaatgt atctgttgca tatcatttcc ggtgatcgtt tgcttactac cacactggtg
     5041 aaagaataag ataaatgatg gaatttttcc gggcgggtac caaagtaccc gccttttttt
     5101 tgatcgtgga ttccggaacg caaccac
//
LOCUS       DAOMAX010000190         5126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2743_length_5126_cov_4.682508, whole genome shotgun sequence.
ACCESSION   DAOMAX010000190 DAOMAX010000000
VERSION     DAOMAX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2743_length_5126_cov_4.682508"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..384
                     /locus_tag="PK742_09625"
     CDS             40..384
                     /locus_tag="PK742_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQU76888.1"
                     /translation="MNVENAQAQMRKGVLEFCVLAIISEGEIYPSDIIGRLKDSRLLV
                     AEGTVYPLLNRLKNMELLSYQWVESNSGPPRKYYQLTDKGRQFLTELHKTWQELAHAV
                     AVNSKHLKTMEQ"
     gene            381..905
                     /locus_tag="PK742_09630"
     CDS             381..905
                     /locus_tag="PK742_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQU76889.1"
                     /translation="MNRILQANIGGQVFPMEEPAYRKLDRYLNHIRRKQGADGGEIIA
                     DMEQRIAELLTDELGNKAFVSLPMVDRVIQTMGQPEDFDTDERRTYEAASDRGFPGFT
                     RDTSNKILAGVCAGIGNRFDIDPLWVRLAFVVSVLLGGAGFLIYLILWILVPASGKKK
                     KSAELVNHVNYAVK"
     gene            902..2461
                     /locus_tag="PK742_09635"
     CDS             902..2461
                     /locus_tag="PK742_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQU76890.1"
                     /translation="MNKLIQVNIGGLVFQIDEGAYHKLDKYLSSIRNRYAQEEGGEDI
                     LHDIEARIAELFIERTGTNGAVMMADVDHVIEQMGRPDDFETADEPISEENPGRKRSR
                     RFFRDGEHRQLGGVCAGFGAYFDIDPLWIRLLFVAAFFFGGSGILLYIVLWVIMPEAK
                     TSSEKLEMRGERVNVSNIERTVKGGAREIRTKANEIGDEMKQVFSEENMRRTSNSVGE
                     FLENIVETIKPVVRFIFKIFVFLIVLACLASLVAIAVVFLTDVGEPAAHIDFLTSHFF
                     ETLRQGQILIFGGIALAAIPLLSLLVRGVKFLLNVRLNFKAFDWTMLVLWLVCLTGVT
                     VVGIQLAQDFEYDGKQTNRISVVQPVAGPLILQVDDSEKDAIRSEWSKGSHWKHVEVY
                     DEASIYDDIDITIERSDDTLYSFTLIRFAHGDSRDEARSRASQVSYELGQIDSIIRIP
                     SVVKLKEGELWRGQQVDLIVRVPMDKTIILDSRIDDFLRYNDYTEDLDHDQLFNRKLR
                     MTATGLRPVYE"
     gene            2478..2981
                     /locus_tag="PK742_09640"
     CDS             2478..2981
                     /locus_tag="PK742_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76891.1"
                     /translation="MQFSSAQWHLLFNHFPIILSITAAGLMVLAFFIRNKGFVQTAMF
                     VLVLAGISAIPAFQTGEGAEETVEELAGVDHQQIHVHEHQAQTGMRVTVGAGIIALIG
                     MLWMRSKKVQPMLPVVLVAIAAFTSSYFMTMVGHSGGLIRHPEISADGGGSTPVMQND
                     HRDDDDD"
     gene            3007..4053
                     /locus_tag="PK742_09645"
     CDS             3007..4053
                     /locus_tag="PK742_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76892.1"
                     /translation="MHRPVSRAFFVLFIPIFWAWSSCADKQQEVIRSFYHWQTTLDLS
                     TEEIQELSSLGVHDLYVKYFDVIWDEEAAQPRPVAKLQTVQVPDELSIHPVIFLTNET
                     LEHLDMDGVNDLAWRMCKLMDQIHTGLSKDPPAIIQIDCDWTEGTRDKYFQLLKQMQT
                     YKRFEETIWSATIRLHQYAYPQQTGVPPVDRGLLMFYNMGNIEDPDAAHSIYDPSTAE
                     AYLKQTDHYPIPLDAGIALFSWGLYFHHRELQQIFYPLYADELLNESLFTTVDDTHFA
                     ATGEGYFRGRFVQPGDTIRIESMSPELGLKSAEQLSHYLHDHPLSVILYHTDPVILQH
                     YTHEELDSVFSAFE"
     gene            4016..>5126
                     /locus_tag="PK742_09650"
     CDS             4016..>5126
                     /locus_tag="PK742_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76893.1"
                     /translation="MKNWTRSFPLLSSLLILFPAQTGNSCGWFYDDDEISYRPYNQYI
                     IDLPEWAWFYYSAHALDFGDFTDTPFDYNLYSQKEENIRFWSDYYQGKYAETFISEMV
                     YADPSDLKQLWDDGISKKKSQKWSNPLAQRWQKGEGLNELKYLLFAHEVVPYVQELDW
                     WSNETQDLETMHILQEQAVDLEAQENDPGIRQRYAFQAIRLAHYAGNFDDCLLLFEQL
                     AKNTASVNDLYWWCLSLKAGAHLRKGEFASAAYYNALVFEHSRAKRQYALRDFYIDSD
                     ATWQQALSMCRNEQEQKTLWLMAGYKQPNAAPMCMEALLQLDPAAPELELLITREVEQ
                     IQREYLPERDFGLQAENGWLPPATSDRIARLQQILQ"
BASE COUNT         1335 a         1252 c         1326 g         1213 t
ORIGIN      
        1 ggtattgcat agtaccatag tatgtcatag ctttgcatca tgaacgtaga aaatgcacag
       61 gcccaaatgc gcaaaggtgt gctggagttc tgtgtcctgg ccatcatcag tgagggcgag
      121 atctatcctt cagacatcat cggccggttg aaagacagcc ggttgctggt ggcagaaggt
      181 accgtctatc ccttgctcaa caggctgaag aacatggaac tgctcagcta ccagtgggtg
      241 gaaagtaatt cgggtccgcc cagaaagtac taccagctca ccgacaaggg caggcagttc
      301 ctcaccgaac tccacaagac ctggcaggaa ctggcacatg cagttgctgt caactcgaaa
      361 caccttaaaa ccatggaaca atgaacagga tattacaggc caatattggc ggacaggtat
      421 tcccgatgga agaacctgca tacaggaaac tggatcgcta cctcaatcac atcagaagga
      481 aacagggtgc cgatggaggt gagatcatcg cagacatgga acagcgcatt gctgaactgc
      541 tgaccgatga actgggtaat aaggcttttg tcagcctgcc gatggtggac cgtgtcatcc
      601 agaccatggg gcaacctgaa gattttgata ccgacgaaag aaggacctat gaagccgctt
      661 ccgacagggg tttcccgggt tttacccgcg acacttccaa caagatcctg gctggtgtgt
      721 gtgccggtat cggaaaccga tttgatatcg atccgctgtg ggtacgactg gccttcgtgg
      781 tctccgtact cctgggcgga gccggttttc tgatctacct gatcctctgg atactggtgc
      841 ctgcttccgg taaaaaaaaa aaatcagctg aacttgtaaa ccacgttaac tacgcagtaa
      901 aatgaacaag ctcattcagg tgaacatcgg cggactggtc tttcagatcg acgaaggtgc
      961 ctaccataaa ttagacaagt acctcagctc catccgcaat cgctatgcgc aggaggaagg
     1021 tggggaagat atcctgcacg atatcgaagc ccgcattgcc gaactgttca ttgaacgcac
     1081 cggtaccaat ggtgcggtca tgatggctga tgtggatcac gtcatcgaac agatgggtag
     1141 gccggatgat tttgaaacgg ccgatgaacc gatcagtgaa gaaaacccgg ggagaaaacg
     1201 gtctcgcaga tttttccgcg atggtgaaca ccgtcagttg ggtggtgtat gcgccggctt
     1261 tggtgcctac tttgatatcg acccactctg gatccgcctg ctgttcgtag ccgcattctt
     1321 ctttggtggc tccggaatac tcctgtacat agttctgtgg gtcatcatgc cggaagcgaa
     1381 aacctccagt gaaaaactgg agatgcgtgg agaacgggtc aatgtatcta acattgaaag
     1441 aactgtgaaa ggtggtgccc gtgagatacg caccaaagcg aatgagatcg gtgatgagat
     1501 gaaacaggta ttctctgaag agaatatgcg acgcacaagc aactcagtag gtgagtttct
     1561 ggagaacata gtggagacca tcaaacctgt agtgcgtttc atatttaaga tctttgtatt
     1621 cctgatcgtg ctggcctgtc tggcatcgct ggtggccatt gcggtggtct tcctgacaga
     1681 tgtaggtgaa cctgcagccc acatagattt tctgacaagt catttctttg agaccctgcg
     1741 tcagggacag atcctcatct ttggtggtat cgctctggca gctatcccgc tcctgtcctt
     1801 gctggttcgc ggcgtcaaat tcctgctgaa tgtcagactg aatttcaagg catttgactg
     1861 gaccatgctg gtgctgtggc tggtgtgcct gaccggtgtg accgtggtag gcatccagct
     1921 tgcacaggac tttgaatacg acggcaaaca aaccaatagg atcagcgtgg tacaacccgt
     1981 tgcaggtccg ctgatcctgc aggtggacga ttctgaaaaa gacgccattc gaagcgaatg
     2041 gtctaaaggc agccactgga aacacgtgga agtgtatgat gaagcaagca tctatgatga
     2101 catcgacatc accatcgaac gcagtgatga tacactctac agtttcaccc tgatcagatt
     2161 tgcacatggc gacagccgtg acgaggcaag aagcagggct tcacaggtca gctatgaact
     2221 cgggcagatc gattccatca tccgcattcc atcggtggtc aaactaaagg aaggtgaatt
     2281 atggagaggg cagcaggtgg acctgatcgt ccgggtacca atggacaaga ccatcatcct
     2341 ggacagccgc atcgatgact tcctgcgcta caacgattac accgaagacc tggaccacga
     2401 ccagctgttc aatcggaaat tgcgcatgac cgccaccggt ctgcgtccag tatatgaata
     2461 atatctaaat tcacattatg caattctcaa gcgcccaatg gcatcttctc ttcaaccatt
     2521 tcccgatcat tctcagcatt actgctgcag gtttaatggt tctggctttc tttattcgta
     2581 acaagggttt tgtgcaaaca gccatgtttg tattggtact ggcgggcata tcggccattc
     2641 cggcctttca aaccggagag ggtgcagagg aaactgtgga agaactggca ggtgtagatc
     2701 atcagcagat ccatgtccat gaacatcagg cacagacagg tatgcgtgta actgtcggag
     2761 ccggcatcat agccctcatc ggtatgctat ggatgcgcag taagaaagta cagcccatgc
     2821 tgccagttgt gctggtagcc attgcagctt ttacttcttc ttacttcatg accatggtgg
     2881 gacatagcgg cggactgatc cgtcacccgg agatcagcgc agatggaggt ggcagcaccc
     2941 ccgttatgca aaatgatcac cgggacgacg acgatgactg aactgcattg actaccttag
     3001 acaggcatgc accggcctgt ttccagagca tttttcgtcc tgttcattcc cattttctgg
     3061 gcctggtctt cttgtgcaga taagcagcag gaggtcattc gcagcttcta tcactggcag
     3121 acaacgctgg atctgagtac agaggagata caggaattgt catcactggg agtacatgac
     3181 ctgtatgtga aatattttga tgtgatctgg gatgaggagg cggcgcaacc tcgtccggtg
     3241 gcgaagttac agaccgtgca ggtgccggat gaactgtcca tacatccggt catcttcctg
     3301 accaatgaaa cactggagca cctggacatg gacggggtca atgacctcgc ctggcgcatg
     3361 tgcaaactga tggatcagat ccataccggt ctgtcgaaag atccgcccgc catcatccag
     3421 atagactgcg actggaccga aggcaccaga gataaatact ttcagctcct gaaacagatg
     3481 cagacctaca aaaggtttga agaaacgatc tggagtgcta ccattcgcct gcatcagtat
     3541 gcctatcctc aacaaacagg agtacctccg gttgaccgtg gcctgctcat gttctacaac
     3601 atgggaaaca tagaagaccc cgatgcagca cattccatat atgatccatc cactgctgaa
     3661 gcttatctga aacagacaga tcattatccc attcctctgg atgcgggcat tgccctgttc
     3721 agctggggac tctacttcca ccatagggaa ctgcagcaga tattctatcc cctgtatgcc
     3781 gatgaattat tgaatgagtc gctgttcacc acggtcgatg acactcattt tgctgcaaca
     3841 ggagaaggtt atttccgcgg acgtttcgta cagcccggcg ataccatccg gatagagagc
     3901 atgtcgcctg agcttggtct gaagtcggca gaacagttat cacactattt gcacgaccat
     3961 ccgctttcgg taattttata ccatacagat cccgtcatcc tccaacacta tacccatgaa
     4021 gaactggact cggtcttttc cgcttttgag tagcctgctc atcttatttc cagcccagac
     4081 aggcaattcc tgtggctggt tctatgatga tgatgagatc tcttatcgtc cctacaacca
     4141 gtacatcata gatcttcccg aatgggcctg gttctattat tcagcacatg cactggattt
     4201 cggcgacttt acagatacac cttttgacta caacctgtat tctcagaaag aggagaacat
     4261 tcgtttttgg tctgactatt atcagggaaa atatgcagaa acgttcatca gtgaaatggt
     4321 ctatgctgat ccatcagatc tgaagcagct atgggatgat ggtatcagta aaaagaagtc
     4381 gcagaaatgg agtaatcccc tcgctcaacg atggcagaaa ggagaaggac tcaatgaact
     4441 gaagtacctg ctgtttgcgc acgaggtggt gccctatgta caggaactgg actggtggag
     4501 caacgagaca caggacctgg agaccatgca tatcctgcag gaacaggctg tggatctgga
     4561 agcacaggag aatgatcccg gcatccggca gcgctatgct tttcaggcca tcaggctggc
     4621 gcattatgca ggaaactttg atgattgttt gttgctgttc gagcaactgg caaagaatac
     4681 tgcttccgtc aatgatctgt actggtggtg cctttcgctg aaggcaggag cgcacctgcg
     4741 caagggcgaa tttgcatctg cggcatacta caatgcgctg gtgtttgaac acagcagagc
     4801 gaaaagacaa tacgccctgc gtgattttta tatcgacagc gacgctacct ggcagcaggc
     4861 tttgtctatg tgccgcaatg aacaggaaca aaagaccctc tggctgatgg ctggatacaa
     4921 acaaccgaat gcagcaccca tgtgtatgga agcgctgttg caactggatc ctgcagctcc
     4981 ggaactggaa ctgctcatta ccagagaggt ggaacagata caaagagagt accttcctga
     5041 acgggacttt ggcctgcagg cagaaaatgg ctggctgcca cctgcaacca gtgatcggat
     5101 agcaagactg caacagatct tgcaag
//
LOCUS       DAOMAX010000191         5117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2756_length_5117_cov_3.865073, whole genome shotgun sequence.
ACCESSION   DAOMAX010000191 DAOMAX010000000
VERSION     DAOMAX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2756_length_5117_cov_3.865073"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PK742_09655"
     CDS             complement(<1..361)
                     /locus_tag="PK742_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76894.1"
                     /translation="MKKGLLVTAWLLIAAMPVLACTSCGCSGGGTTLGMQLVTVRNYA
                     GMIWQHTGLTVYEQGELHSSFASDTWQVYGSLQLPGKWHMQARLPLVWNRVTDHSPEG
                     DQTENLFAAGDLNLMAGY"
     gene            complement(361..774)
                     /locus_tag="PK742_09660"
     CDS             complement(361..774)
                     /locus_tag="PK742_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HQU76895.1"
                     /translation="MKKIILPAVALAAVFSALTSCTQDEEPTPTPEAYSVTINIIEPS
                     EGETITSGSTVHLEATFENDNTIHNIGVWMVGEMMNDTLYSYENHVHADQYYEFHEHT
                     MPMVMEAQEYKVIFASWDEDPAERIEKHVHVNIEP"
     gene            complement(793..1197)
                     /locus_tag="PK742_09665"
     CDS             complement(793..1197)
                     /locus_tag="PK742_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76896.1"
                     /translation="MVKLKIVAVSAVLLVLLVITEMGSLLSLVAFRTNQDFIAANLCV
                     NRDRPELECNGKCQLIKTLEEQQPSGQPKAVPANDLIIPLFHTAYQNVSPLVDEAVCE
                     YLTIMISVPAVPFKETATPPPDRISAYRSLFV"
     gene            1321..1887
                     /locus_tag="PK742_09670"
     CDS             1321..1887
                     /locus_tag="PK742_09670"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HQU76897.1"
                     /translation="MRSIFFSILCLFTLSVMAQDKQIDLSAWGLPSFPLDEETQLISF
                     SDVVDVEGVSAADLYQLGLNWIGDYYKNSSSVMQVKDADNGILEGKHSFYVMRDVNGQ
                     MVKGDLIKYQFNIRFRDGRYKYTITRINVQKSAYYGIEEWINDENKISDPEIQDFLEQ
                     IHTFFTEEYIPAMTEGIQPKKEAKEEDW"
     gene            2015..2941
                     /locus_tag="PK742_09675"
     CDS             2015..2941
                     /locus_tag="PK742_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU76898.1"
                     /translation="MSATLKPKKQGKELPVVKKDTRPRRKALKKTPSFRISKVLFPTD
                     FSESSADMLRMVVNFCEKLQADLVVFHAYKIALVDEYMPSAMVDSMLDEGEKAALEKL
                     HELTSGLETSVQIDYQVKMGFTAESISEAANTSGADLIMMGTNGCNSLEDRMFGTVSW
                     NTIKHAEVPVLTIPEGAAVPAFQNIIFPFECTDKDLEIIQFAVEIASLFDSKIHLIHF
                     LLEGSVVNKTILDKINHRYHKEIREEKLQIQILADKNITDGIVKYAEGVSADMIIMVT
                     HHRGLMATLFHMSTTRNIVLYSSVPLLAFKAE"
     gene            2983..3690
                     /locus_tag="PK742_09680"
     CDS             2983..3690
                     /locus_tag="PK742_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQU76899.1"
                     /translation="MKAKITVPDCEHCKNRSSTLFHFCHTNETEVINENKTCALYKKG
                     QVVFHEGANSYGLYCLNSGKVKLYKMGPDGKEQILKIVTPGDFIGYGALLSSAPYRVT
                     AEVIQDAVICFVPKEIITQMFKENPNFSEGMVKLLTKTLDETVEKMADIAYKPVRGRI
                     AEALLILKETYKDDNNPDGVINITREDLASYVGTVKETAIRVLKDFKEEGLIVTESHS
                     IKIIDPKGLTRVCELYD"
     gene            complement(3747..4277)
                     /locus_tag="PK742_09685"
     CDS             complement(3747..4277)
                     /locus_tag="PK742_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76900.1"
                     /translation="MEIPHVLPVLLFGSDEKLPAPYGLLSAVLHAHSGVRYLILIGLI
                     AAVFLAFNAMRKDQAWDGSPKRLGRLTMILADIQLLMGIFLWVYFIYLNTGLKMKRLK
                     DMLGMQEVRFMAVEHAVMMFIAIVLIHIGYIRAKKAPSVRQANKSQFLFYLIALIMIL
                     VAIPWPFYGHMGRGWF"
     gene            complement(4295..4600)
                     /locus_tag="PK742_09690"
     CDS             complement(4295..4600)
                     /locus_tag="PK742_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76901.1"
                     /translation="MLNILSKFLGGGKSGGGADRVAAFIGAMEKDLHLDAQQKTAVEQ
                     ALRQFMQDRKSAKQSGNKDAMKDGRDQFREAISAILRDDQEKIFAGKFQEYKQLLKG"
     gene            complement(4680..>5117)
                     /locus_tag="PK742_09695"
     CDS             complement(4680..>5117)
                     /locus_tag="PK742_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76902.1"
                     /translation="LYGVSATLRADITSGFSAFSTITYTYGRVETDTTPYPLDHIPPV
                     FGRTGCTLQLDKFRCDAYVMYQGWKRIEDYNIVGGEDNEQYATPYGMPAWYTLNLKAG
                     YAISEKLTLQAGIDNITDQHYRVFASGVSAPGRNLYLTVRASI"
BASE COUNT         1458 a         1155 c         1165 g         1339 t
ORIGIN      
        1 ggtatccggc catcaggttc agatctcctg cagcgaacag gttttctgtc tgatctcctt
       61 ccgggctatg atcagttact ctgttccaga ccaatggcag gcgggcctgc atatgccatt
      121 ttccgggaag ctgcaaagaa ccatatacct gccaggtatc agaagcgaag ctgctgtgca
      181 gttcaccctg ttcatatacg gttagtccgg tatgttgcca gatcattccg gcgtagtttc
      241 tgaccgttac cagttgcata cccagggtag taccaccacc ggagcagccg caacttgtgc
      301 aggccaggac cggcatggct gcaatcagca gccatgccgt taccaatagc cctttcttca
      361 ttatggttca atattcacat gcacatgttt ctcgatccgt tcagcaggat cttcgtccca
      421 ggaggcaaat atgaccttgt attcctgtgc ttccataacc atcggcatcg tatgctcatg
      481 aaactcataa tattggtctg catgcacatg attctcatag ctgtacaggg tgtcgttcat
      541 catttcgccc accatccata cgcctatatt gtgaatggta ttgtcattct caaaggtagc
      601 ttccagatga acggtgctgc ctgaagtgat ggtttctcct tcagaaggct caataatgtt
      661 aatggttaca gaatatgcct caggtgtagg tgttggttct tcatcctgtg tacaagaggt
      721 cagggcactg aaaacagccg ccagtgccac agcgggcaat atgatttttt tcattttatt
      781 tgattgtatc agttaaacaa acagagatct gtaagcactg atacgatcag gaggtggggt
      841 agcagtttcc ttgaacggaa ctgcaggtac agagatcatt atggtaaggt attcgcaaac
      901 cgcttcatcc accaatggtg acacgttctg gtaagcggta tgaaacaggg gtataatgag
      961 gtcgtttgca ggaactgcct taggctgacc ggatggctgt tgctcttcca atgttttgat
     1021 cagctggcat ttaccgttac attccagctc aggcctgtct cggttcacgc acaggttggc
     1081 tgcaatgaaa tcctgattgg tccggaaagc caccaaagac aacaatgacc ccatctcggt
     1141 gatgaccagc aacacgagta atacggcaga cactgcaact atcttcagct tcaccataca
     1201 ggaatacagc aaaggtaacg cacataggca caaggaaaaa agagattatt atcaccaaat
     1261 attggcaata tatttgctgt cagacaactg tctgaatatt ttaaacttaa aactagatac
     1321 atgcgcagca tctttttttc cattttatgc ctattcacct tgtctgtcat ggcgcaggat
     1381 aaacaaattg atctgagtgc ctgggggctg ccatcgtttc cgctggatga agagacccaa
     1441 ctgatatctt tcagcgatgt ggtggatgtg gaaggcgtgt ctgccgccga cctgtatcag
     1501 ctggggctga actggatcgg agattactac aagaactcat cttctgtcat gcaggtgaaa
     1561 gatgctgaca atggcatcct ggaaggcaag cacagctttt atgtgatgcg tgatgtgaac
     1621 ggccagatgg tgaaaggaga tcttatcaaa taccagttca acattcgatt ccgcgacggc
     1681 agatataagt acaccattac ccgcatcaac gttcagaaat cagcttatta tggaattgaa
     1741 gaatggatca atgatgagaa caagatctct gatccggaga tacaggactt tctggaacag
     1801 atccacacct tcttcaccga agaatatata ccggcaatga ccgaaggcat acaacctaaa
     1861 aaagaggcca aagaagaaga ctggtaagct tcattcaaac gttaagaact cataaaagtc
     1921 actctctgag tgacttttat catttatacc tctgatgccc atcatgtccg cacctctgat
     1981 ataagaagca attttgaggg taatcagaat aattatgtct gcaacactca aaccaaagaa
     2041 gcagggtaaa gaattacctg ttgtaaagaa ggataccaga cccaggcgca aagcactgaa
     2101 aaagactccg tcttttcgca tcagcaaggt tctttttcca acagacttca gtgagtccag
     2161 tgctgatatg ttgcgcatgg tggttaactt ttgtgagaaa ctgcaggcag atctggttgt
     2221 ttttcatgcc tacaagatcg ctctcgtaga tgaatatatg ccgtctgcta tggtagatag
     2281 catgctcgat gaaggcgaga aagcagccct ggagaaactg cacgaactta ccagtggtct
     2341 ggaaacctct gtgcagattg actaccaggt taaaatggga tttacagctg aaagcatcag
     2401 cgaagctgca aatacctcag gagctgacct gatcatgatg ggtacgaacg gttgcaacag
     2461 cctggaagac cgcatgttcg gtaccgtttc ctggaacact atcaagcatg ctgaagttcc
     2521 tgttctgacc ataccggaag gggcagctgt acctgcattt caaaacataa tattcccatt
     2581 cgagtgcaca gacaaagacc tggagatcat acaattcgca gtagagatcg cttccctgtt
     2641 cgatagtaag atccacctga tccattttct gctggaaggt tctgtagtca ataaaaccat
     2701 acttgacaag atcaatcacc ggtatcacaa agagatcagg gaagaaaagt tgcagatcca
     2761 gatcctggcc gataaaaaca tcactgatgg tattgtcaag tatgcagaag gtgtgtctgc
     2821 tgatatgatc atcatggtga ctcaccaccg cggactgatg gccactcttt tccatatgag
     2881 cactaccaga aacattgtgc tgtattccag tgtgccgctg ttggcattta aggcagaata
     2941 gcagtagatt tgtccacaag ggcaaactca aaaaaggcat gtatgaaagc caagatcaca
     3001 gttccggatt gtgagcattg caaaaataga agttccactc tttttcattt ctgccatacc
     3061 aacgagacgg aagtaattaa cgagaacaaa acctgtgctc tatataaaaa aggacaggtg
     3121 gtttttcatg aaggggccaa cagttatggt ctgtattgtc tgaactcagg caaggtcaag
     3181 ctatataaga tgggtccgga tggtaaagag cagatactta agatcgtaac acctggagac
     3241 tttatcggat atggtgccct gctttcttct gctccttacc gggtcactgc agaagtgatc
     3301 caggatgcag tgatctgttt cgtgccaaaa gagatcatta cccagatgtt caaggaaaat
     3361 ccgaattttt ctgagggcat ggtcaagctg cttaccaaaa cgctggatga gaccgtagaa
     3421 aaaatggcag atattgccta caaaccggta agaggaagga ttgccgaagc acttctgata
     3481 ctaaaagaga cctacaagga tgacaataat cctgatgggg tcatcaatat tacccgtgaa
     3541 gatctggcct cttatgttgg aacggtgaaa gaaactgcaa tcagggtatt gaaagacttc
     3601 aaggaagaag gactgatagt aacagagagt cattctatca agatcattga cccaaaaggc
     3661 ctgaccaggg tctgtgaatt atacgactga aaaccaaaac aggatcaaaa aagccgggcg
     3721 atctgatcgt ccggcttttt ttgttttcag aaccagccac ggcccatatg tccgtaaaac
     3781 ggccatggga tggctaccag gatcatgatc aatgcgatca ggtaaaacag gaattgtgat
     3841 ttattggcct gtcttacaga aggagctttc ttagcacgaa tgtatccgat atgaatgagt
     3901 acgatggcaa tgaacatcat gaccgcatgt tccacagcca tgaatctgac ctcctgcata
     3961 cccagcatat ctttcaggcg tttcattttt aatcctgtat tcagatagat aaaatacacc
     4021 cacagaaata tgcccatgag caactgaata tcggccagga tcatggtgag tcgtcccaat
     4081 ctcttgggcg atccatccca agcctggtct tttcgcatag cattaaaagc caggaatacg
     4141 gcagcaatca aaccgatcag gatcagatag cgcacgccgc tgtgggcatg caaaacggca
     4201 gataacaggc catacggagc cggtaatttt tcatcagatc cgaataataa aacagggagt
     4261 acgtgtggta tttccataat tggattttag gttttcagcc ttttagcaat tgtttgtatt
     4321 cctgaaactt accggcaaag atcttttcct gatcatcccg caaaatagca gagatcgctt
     4381 ctctgaattg atctcttccg tctttcatag catctttgtt tccgctttgc ttagccgatt
     4441 ttctgtcttg catgaattgc ctgagcgcct gctctacggc tgttttctgt tgtgcatcca
     4501 gatggaggtc tttttccata gcaccaatga atgcagcaac ccggtcagct ccgccacctg
     4561 atttaccacc acctaagaat ttggataata tattcagcat ttcttttgtt tgatgcctaa
     4621 aaatagacag atgtcaccgt tatgtcaaat cggtaacatc tgtctgtatt cctgcagaat
     4681 caaatagaag ctctgacagt caggtacagg tttcgacccg gggcactcac accggatgca
     4741 aacaccctgt agtgctggtc ggtgatattg tcaataccgg cctgcaaggt cagtttttcg
     4801 ctgattgcgt aacctgcttt caggtttagg gtgtaccatg caggcatgcc ataaggagtg
     4861 gcatactgtt cattgtcttc accaccaaca atattataat cttctatccg cttccatccc
     4921 tggtacatca cataggcatc gcagcggaac ttatccagct gcagggtaca gcctgtgcgg
     4981 ccaaagacag gcggaatatg atccagcgga tatggtgtag tatcggtttc cacccttcca
     5041 taggtgtagg taatggttga aaatgcagaa aagccagatg tgatatcagc cctaagtgta
     5101 gcagaaacac catacag
//
LOCUS       DAOMAX010000192         5093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2783_length_5093_cov_4.460897, whole genome shotgun sequence.
ACCESSION   DAOMAX010000192 DAOMAX010000000
VERSION     DAOMAX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_5093_cov_4.460897"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PK742_09700"
     CDS             <1..1209
                     /locus_tag="PK742_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQU76903.1"
                     /translation="TMEKLQEEILSSRPEFLCLYVNLMTRVNIVRILHWMQEQMPETV
                     LIVGGPEVRYHAEQLLDHGAGYVVVGEGEQTCLELIQELQQGKEPLQVSGLIFRTADG
                     TIHRSGERHKIKDLDELPLPARDKIDLRLYLDAWKQRHGMNAVSVNTMRGCPYSCKWC
                     SRAVYGMSYRRRSVTHVVDELLHIKKTWAPDTLWFVDDVFTVSHKWLAAFCDEVTGRD
                     AVIPFECITRADRMNEEVIRMLKASGCFRVWIGAESGSQKVIDLMDRRVEVEQVRNMI
                     QLARQHGLEAGTFIMLGYPGETEADIEETIRHLQLSHPDHYTITVAYPIKGTELYAEV
                     EPHFLNAPDWSTTSDRQQDFARTYSRKFYDHAVRYVVNKVETSRTKGFRSVKHRFKAA
                     AAKSMMAISK"
     gene            1242..2267
                     /locus_tag="PK742_09705"
     CDS             1242..2267
                     /locus_tag="PK742_09705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU76904.1"
                     /translation="MQLLILTPGFPQDEQDTTCIPFLQDFVLGMSEHLGAPHIKVISF
                     QYPFSEANYTWHEVEVFSAGGRNKKGMRRMFTWRRVMKQAGLWMTENTVIHSFWLTEA
                     TLIGQQLQEKYGCRHIATIMGQDVLPSNRYLKKLSAEKMYIVAPNERTASLLHSQKLQ
                     ESAIIPHAIMPLIFARQQRTTDLIWVGSFIALKQPEQFLLLVKELLSTNPGISCCMIG
                     EGPLLNKTKQRAEREELPVEICGKLERPDVLQRMERSRILVHTSNYEGHSTVISEALS
                     KGCRVVCYDVGHTHSDLIHTAGNLEHMTRIVGELLDTDMEYLPVYPDQMSEMLQQYLD
                     LYSGRTY"
     gene            2339..3265
                     /locus_tag="PK742_09710"
     CDS             2339..3265
                     /locus_tag="PK742_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQU76905.1"
                     /translation="MKKVLKWIGIIILVLVVIIVAAMFMFRSKYKSLANERFEVNVPD
                     IALQTDSAGMARGEMLATALCTGCHGGDLAGKDFFNDKTIGVVYSANLTPGGPTAAWS
                     TEDFVRAIRYGVRPDGSGLFIMPSLEYNHMSDYDLGCLIAYLRSLPASDNPSPPAEFT
                     FMAQVMAGAGLFGDLYSASVLDLQDGSARERPEVGETLAYGEYVMLISGCQSCHGEAF
                     NGAKSPDPVSPPGGNITPAGNIGNWNYDQFAETMWTGKTPEGKEMDPKFMPWDGIRLL
                     GDTEKKALFLYLQNLPPKEDSEDLARWKAKNS"
     gene            3465..4964
                     /locus_tag="PK742_09715"
     CDS             3465..4964
                     /locus_tag="PK742_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQU76906.1"
                     /translation="MLPRIILFNPRAANHKPRIPNSILSLAATLEGRYDYVMVDGNLE
                     AEPYEKMEAYVRQGYDTLAVTVMPGPQLKQAIPYTKKLKERFPKLVTIWGGYFASNQW
                     QVCMASGFVDYLIYGPGDEAFPSLIRLLHTGGDLGAIPNLIWKLYGMVQKNHKDELYD
                     QDTLLRLPYDTLNRFYPLEKYLGRTWLGEKTIAYHSSVGCPFKCAFCGIVPIYNARWK
                     GKSAAGIYQDIKYLKDQFGGSAIEFHDNNFFVSEKRTVEFSRLIKSEQMIWWGEGRID
                     TIDKYSDQSLALMRDAGCRMIFFGAETGNDEILAMMDKGGTQTGDQIKGFAARMKRFD
                     IIPEYSFVLGTPAATEAQVNSQIDQDIAFIREIKEINPDTEIIIYVYSPVPTEGSELF
                     EACKANGFQFPEQLEDWISPAWESFDLRKNPLTPWLKPYMIDRIRNFETVLNGYSPTV
                     SDIKLMPARKRLMRSLSSWRYRSGFYRAPLEIKALQKISRYRQPEIEGF"
BASE COUNT         1364 a         1167 c         1361 g         1201 t
ORIGIN      
        1 actatggaaa aactgcagga agaaatactg tcttcccggc ctgaattcct ttgcctgtat
       61 gtcaatctga tgaccagggt gaacatcgta cgcatcctgc attggatgca ggaacagatg
      121 ccggaaacag tgctgatcgt tggcggaccg gaagtgcgtt accacgcaga gcagctgctg
      181 gatcatggag ccggttatgt ggtggttggg gaaggcgaac agacctgtct ggaactgata
      241 caggaactgc agcaaggaaa agagcccttg caggtgagcg gattgatctt tcgcacagcc
      301 gatggcacca ttcatcgcag tggtgaaaga cataagatca aagacctgga tgagctgcca
      361 ttacctgccc gggataagat agatctgcgc ctgtatctgg atgcatggaa acaaaggcat
      421 ggcatgaatg ccgtatcggt caacaccatg cggggctgtc cgtacagctg taaatggtgc
      481 agtcgtgcgg tgtatggtat gagttatcgc cggcgcagtg tgactcatgt ggtggatgaa
      541 ttgctgcata tcaaaaagac ctgggcacct gatacgttgt ggttcgtgga tgatgtgttc
      601 accgtcagtc ataagtggct tgctgcattc tgcgatgaag tgaccggtcg tgatgcggtc
      661 attccttttg aatgcatcac ccgtgccgac aggatgaatg aagaagtgat acgcatgctg
      721 aaggccagcg gatgtttccg ggtatggatc ggtgcagaga gtggcagcca gaaggtcata
      781 gacctgatgg acagaagggt ggaagtggag caggttcgaa acatgataca gcttgcgagg
      841 caacacggac tggaagccgg caccttcatc atgctgggct atccgggcga aacggaagca
      901 gatattgaag agaccatccg gcatctgcag ctgtcgcatc ctgatcatta caccatcaca
      961 gttgcctatc cgatcaaggg taccgaactg tatgccgaag tggagccgca tttcctcaat
     1021 gcgcctgact ggtcaaccac cagcgacagg cagcaggatt ttgccagaac ctattccagg
     1081 aaattctatg accatgcagt gcgctatgtg gtcaacaagg tggagaccag cagaacaaaa
     1141 ggtttccgct ctgtcaaaca tcggttcaag gccgcggctg caaaaagcat gatggccatc
     1201 agcaaataag accggaaacg gttcgtttag tacaagatct catgcaatta ctgatactga
     1261 cacccggatt tccgcaggat gaacaggata ccacctgcat accttttctg caggattttg
     1321 tgttgggtat gtctgagcac ctgggggcgc cccatatcaa ggtgatcagc tttcagtatc
     1381 cgttcagcga ggcaaactat acctggcatg aagtggaagt tttttctgca ggagggcgaa
     1441 acaaaaaggg tatgcgccgc atgttcacct ggcgcagggt gatgaagcag gcaggcttat
     1501 ggatgacaga aaatacggtc atccattcct tctggctgac cgaagccaca cttataggtc
     1561 agcagcttca ggaaaagtac gggtgccggc acattgctac catcatgggt caggatgtgt
     1621 tgccatctaa ccgttacctg aaaaaactat cggcagaaaa gatgtacatc gttgctccga
     1681 atgaacgaac cgcttcctta ttgcattctc agaaattgca ggagtcggcc atcatccccc
     1741 atgccatcat gccgctgatt tttgccaggc aacaacgaac cactgacctg atctgggtgg
     1801 gttctttcat tgcgttaaag caaccggaac agtttctcct gctggtgaaa gaattgctgt
     1861 caacaaatcc gggcatcagc tgctgtatga tcggtgaagg cccgctgctg aacaagacaa
     1921 aacagagagc tgagagagaa gagctgccgg ttgaaatttg tggaaagctg gaacggcctg
     1981 atgtattgca acgtatggaa agaagccgga tcctggtgca taccagtaac tatgaaggac
     2041 atagtacggt tatcagtgaa gcactgtcta agggctgcag ggttgtttgc tatgatgtgg
     2101 gacataccca ttcagacctg atccatacag ccggcaacct ggaacatatg acaaggatcg
     2161 tgggtgaact gctggataca gatatggaat acctccctgt atatccggat cagatgtctg
     2221 agatgctgca gcagtacctt gatctctatt ctggtagaac atactgaact gcctgatcct
     2281 gtttggattt tccatttttt ttttgcatct ttctgataca ctcaaaaccc aaccaaccat
     2341 gaaaaaagtc cttaaatgga tcggtatcat catactggtc ctggtagtca ttattgtggc
     2401 ggccatgttc atgttcagaa gcaagtacaa aagtttggcc aatgaacggt tcgaggtcaa
     2461 tgtaccggat attgctttgc agacagattc agccggaatg gctcgcggtg aaatgctggc
     2521 tacagcatta tgcacaggat gccatggcgg agatctggcc gggaaagatt ttttcaatga
     2581 caagaccata ggtgtggtct attctgccaa tctcactccc ggtggtccta cagcagcatg
     2641 gagcacagaa gattttgtca gggccatcag atatggtgtt cgaccggatg gcagcggatt
     2701 gttcatcatg ccatcgctgg agtacaacca catgagtgat tatgatctgg gttgcctcat
     2761 agcttatttg aggtcattgc cggcatctga taatccatcg ccgccggcag agttcacttt
     2821 catggctcag gtcatggcag gagcaggact gttcggcgac ctgtacagtg cttccgtgct
     2881 ggacctgcag gatggatcag ccagagagcg acccgaggtg ggcgaaacac ttgcatacgg
     2941 agaatacgtc atgcttataa gtggttgcca gtcttgccat ggtgaagcat tcaacggagc
     3001 aaaatcacct gatcctgtgt ctcctccagg tgggaatatc acacctgcag gaaatatcgg
     3061 caactggaac tatgaccagt ttgcagaaac catgtggaca ggaaaaaccc cggaagggaa
     3121 agagatggat cccaagttca tgccatggga tggcatcagg ttgctgggtg atacagaaaa
     3181 gaaggcactc tttctgtatc tgcagaacct gccacctaag gaagactcgg aagatctggc
     3241 gaggtggaaa gcgaagaatt catgagttga cagttggcag cttgacctga tgaaatcagg
     3301 acgggcggtt ggcagttaca ttgatacata tttgtaaaat gagatggtaa acaccatctc
     3361 attttcattt gatgcgccac tcagtcaatc tgtcgttata tttgtttgtc ggttacaaat
     3421 gcattttcaa ggggcctttg ccaactgcca accaccaact aacaatgctt cctcgtatca
     3481 ttcttttcaa tccccgtgcc gccaaccaca agccacgcat acccaattcc attttgtcat
     3541 tggcggcaac cctggaagga agatatgact acgtgatggt agatggaaat ctggaggcag
     3601 agccgtatga aaaaatggag gcgtatgtca ggcagggtta tgataccctt gcggtcacag
     3661 tgatgcccgg gccacagctg aagcaggcca tcccttatac gaagaaactc aaagaacgat
     3721 ttcctaaact ggtcaccatc tggggaggat actttgcttc caaccagtgg caggtttgca
     3781 tggcttcggg atttgtggac tacctgatct atgggcccgg tgatgaagcc ttcccttctc
     3841 ttatcagatt attacatacc ggtggcgatc tgggtgccat ccccaacctg atatggaaac
     3901 tgtatggcat ggtgcagaag aaccacaagg atgaactgta tgaccaggac acccttctgc
     3961 gtttgcccta tgacacactg aacaggttct atccgctcga aaaatacctg ggcaggacct
     4021 ggctgggtga aaagaccatt gcctatcaca gcagcgtagg ctgtcccttc aaatgtgctt
     4081 tctgcggcat cgtaccgatc tacaatgcac gctggaaggg aaagagtgcg gcaggcatct
     4141 accaggatat caaatacctg aaagaccagt ttggaggttc tgccatagag tttcatgaca
     4201 acaacttttt tgtttcagag aaacgcacgg tagaattttc ccgactgatc aaaagtgagc
     4261 agatgatctg gtggggggaa ggacgtattg acaccattga taaatacagc gaccagagcc
     4321 tggctcttat gcgggatgca ggttgcagga tgatcttctt tggggcagag acaggtaacg
     4381 acgagatact ggccatgatg gacaaaggag gcacgcagac gggtgaccag atcaaaggct
     4441 ttgcagccag gatgaaacga tttgatatca tacctgagta ttcttttgta ttgggtactc
     4501 cggcagcaac agaagcacag gtgaacagtc agatcgatca ggatatcgcc ttcatccggg
     4561 agatcaaaga gatcaatccc gataccgaga tcatcatcta tgtgtatagt ccggtgccta
     4621 ctgaagggtc tgaattgttc gaagcctgta aggcgaacgg attccagttt cctgaacaac
     4681 tcgaagactg gatcagtcct gcatgggaat ctttcgacct gcgtaagaac ccgctcacac
     4741 cctggctgaa gccgtatatg atagaccgca tcaggaattt tgaaacagtg cttaacgggt
     4801 attcacctac ggtatcagac atcaaactga tgccggccag aaaaaggctg atgcgttctt
     4861 tgagcagctg gcgttaccgg tcagggttct accgggcacc gctggagatc aaagccctgc
     4921 agaagatcag ccgttaccgg cagccggaaa ttgaaggatt ctgatgcgac ggatatacaa
     4981 gtggtacgca catcatatac tgagaccgct gctcgaaagg aggttgcata aaacagtccg
     5041 gtataaaaaa tatggcctgg acatgctgat cgaaccaggc gtttttcatc ccg
//
LOCUS       DAOMAX010000193         5081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2795_length_5081_cov_4.038599, whole genome shotgun sequence.
ACCESSION   DAOMAX010000193 DAOMAX010000000
VERSION     DAOMAX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_5081_cov_4.038599"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK742_09720"
     CDS             <1..253
                     /locus_tag="PK742_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76907.1"
                     /translation="IYMETPYEDYVYIRCRKGQMRVYTSSDEINEDIFTADAGKRTIE
                     YSGQRVVYQLESKPQVGKFVRKMEAFTGEGGGFDDDDDE"
     gene            264..1058
                     /locus_tag="PK742_09725"
     CDS             264..1058
                     /locus_tag="PK742_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQU76908.1"
                     /translation="MKRTIIFLMALVLLGSCNRVPITGRKQVSLLPNSEVVAMSLTEY
                     NSFLSASQVIETGSDATLVNQVAGQIAKAVEEVMTQQGYADLLQDFDWEFHLVQSDEP
                     NAWCMPGGKIVVYTGILPYTKDASGLAVVLGHEVAHAVARHGNERISQQLLAYTGFAA
                     LDVAMSNKPEETRNLILGAAGIGAQVGVLLPFSRTHESEADRLGLIFMAAAGYDPHTA
                     IGFWERMSANGPSIPEFLSTHPSDETRISNIRDVYMPEAMEFYTGK"
     gene            1060..1728
                     /locus_tag="PK742_09730"
     CDS             1060..1728
                     /locus_tag="PK742_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQU76909.1"
                     /translation="MERKKPYSAWQYWQSFLYPVRLDQKNSSYSGEIRVDLVGGRCQL
                     HTGNAIYSYEDRYTSFMHALGVATFEDDCRVLVLGMGLGSIPYLLFRTFHHRGLVTCI
                     EIDPDIIGLAKKYYPPSAPVPDIIQADAVTWLQQNKLTFDLITMDMFIGEEVPYECWQ
                     TSFLSDLKRAVAPGGALLFSRLKEREVEEQDLWKNLAETFPGAAEIPTSGNSIMYWRY
                     EDDH"
     gene            1712..2677
                     /locus_tag="PK742_09735"
     CDS             1712..2677
                     /locus_tag="PK742_09735"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQU76910.1"
                     /translation="MKTIISSRIDEAEHLLIQGEVVAIPTETVYGLAANAMDTDAVLK
                     IFRAKKRPLFNPLILHIGRKEQVHELAYLSAGPLDRLADACWPGPLTLLLPKKETVHD
                     LITAGSPLVAVRMPDHPLTLPLLQRLHFPLAAPSANLFGTVSPTSPVHVAEQLDGKIP
                     MILDGGPCRVGLESTIVKWENDTLHILRTGAITPEIIENVTGLVPFISKEISEKPEAP
                     GQLKSHYAPETPLQCGDLVQLLADSPSQKIAVLCFRQVPDDSRICAHFTLSAAGSEKE
                     AATRLFEGLRTLDKSGADCIVAELLPEKGLGLAVNDRLRRAATVS"
     gene            2712..3101
                     /locus_tag="PK742_09740"
     CDS             2712..3101
                     /locus_tag="PK742_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76911.1"
                     /translation="MRYLRHAGLLVITLTISVLSFAQAEKYQGTMFENMAPASFEWAA
                     NRLATLDQLVTLTDAQKEEVEVINMRYAYRMDALARQTMDPETLKAHREGLFDYRMED
                     YKRILNPEQVAILVAHEEDLKEDNLLY"
     gene            3208..3882
                     /locus_tag="PK742_09745"
     CDS             3208..3882
                     /locus_tag="PK742_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76912.1"
                     /translation="MKKSTLVLAFMAMAAFGSVQAQDMSRGDADKQQQKKENMSQEDR
                     DRMREERQDKKDKVNSNMSDDAQGRAKDMTDKMDEILDLTDAQYRQAMEVNLRYYNQL
                     DQMRARYAGQEDANKEQMKAKKDELSRERLNSYKSFLTSEQWSKFEERRGDAKEEHES
                     KMTKEEKKERMENMTPEEKERMKERKEQKSSTGTSTPRNKGDMDKEKMQEKRGSSSSK
                     SSGSDN"
     gene            complement(3929..4909)
                     /locus_tag="PK742_09750"
     CDS             complement(3929..4909)
                     /locus_tag="PK742_09750"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQU76913.1"
                     /translation="MDVRLMQLIPVYIQELCQDHDYVVIPGLGGFMKRYRPARMHPTS
                     FHFTPPGTTFVFNERLRENDGLLVARVAMRQQIHSMEAERMVTAYTRSQLELLQAGET
                     IQFPGIGRLVRDPEGSMRFTADPTASYEPSAFGLPAFQASPIRKKAMVTAPPEIPVTA
                     RKERRISSVLGWLAAACVVLALLFLPMQNSGGSGSHQLANLFDSIRAQQNEQIALLYA
                     AEQMPAISSVSSTRETNEPTTAVLVIAEPVEQVVTGSGFWLVCGAFKEQANAENQQER
                     LRNSGFEAAVQVSGDLHMVVVPVPQGTDRYAYRQSFAETTGIKGAWLKKE"
BASE COUNT         1396 a         1254 c         1263 g         1168 t
ORIGIN      
        1 gatctacatg gaaacaccat atgaagatta tgtatatatc cgttgcagaa aaggtcaaat
       61 gagggtatat acttccagcg atgagatcaa tgaagacatc tttaccgcag atgccggaaa
      121 acgaacgatc gaatacagcg gtcagcgtgt ggtctatcag ctggaatcca aaccacaggt
      181 tggtaaattt gtacgcaaaa tggaagcctt tacaggagaa ggcggaggtt tcgatgatga
      241 tgatgatgaa taagacttat attatgaaaa gaactatcat ttttctgatg gcattggtat
      301 tgctgggaag ctgtaaccgc gtgccgatta cgggcaggaa gcaggtgagc ctgctaccta
      361 attctgaagt ggttgccatg agtcttactg agtacaacag ctttctgtct gccagtcagg
      421 tcatagaaac cggcagcgat gcaacccttg ttaaccaggt tgccggacaa attgcaaagg
      481 ctgtggaaga agtcatgacc caacaaggtt atgcagacct gttgcaggat tttgactggg
      541 aatttcacct ggtgcaaagc gatgaaccta atgcatggtg catgcccgga ggaaagatcg
      601 tagtatatac tggtatcctg ccttatacca aagatgccag cggactggct gttgtgctgg
      661 ggcatgaggt agcgcacgct gtagcccggc atggcaatga acgcatcagc cagcaactgc
      721 ttgcctacac aggatttgct gccctggatg tagccatgtc aaacaagcct gaagaaacca
      781 gaaacctgat actcggtgcc gccggaattg gtgcccaggt gggtgtgttg ctgcccttca
      841 gcagaacaca tgaatctgaa gctgacagac tggggctcat ttttatggcc gctgccggat
      901 atgacccgca tactgccatt ggtttctggg aacgtatgtc agcaaacggt ccatccatac
      961 cggaatttct cagcacccac cccagcgatg agacccgcat cagcaatatc cgcgatgtgt
     1021 atatgcctga agctatggaa ttctacaccg gaaaataaga tggaaaggaa aaagccgtat
     1081 tccgcctggc aatactggca gagttttctc tatccggtcc ggctggatca gaagaacagt
     1141 tcctacagcg gagaaattcg tgtggatctg gtgggcggaa gatgccagct tcacacaggc
     1201 aacgccattt attcttatga agaccgctat acctccttta tgcatgcact gggtgttgcc
     1261 acctttgaag atgactgcag ggtgctggtt ctgggtatgg gtctgggctc tatcccctac
     1321 ctgcttttcc ggacattcca tcatcgcggt ctcgttacct gcattgagat cgatcccgat
     1381 attatcggtc tggcaaagaa gtattatccc ccatctgctc ctgtaccgga tatcatacag
     1441 gcagacgcag ttacctggct gcagcaaaac aagctgactt ttgacctgat caccatggat
     1501 atgttcatcg gtgaagaggt gccttatgaa tgctggcaaa cctctttcct gtctgacctg
     1561 aaacgggcag tggcacccgg aggtgcactt cttttcagca ggctgaaaga acgggaggtg
     1621 gaggaacagg atctgtggaa aaatctggca gagacctttc ccggagcagc ggagattcca
     1681 acaagcggaa acagcatcat gtactggagg tatgaagacg atcattagta gccggataga
     1741 tgaagccgaa cacctgctca tacagggaga ggtggttgct atccctaccg aaacggtgta
     1801 tggactggct gccaatgcca tggatacaga tgcggtattg aagatctttc gtgcgaaaaa
     1861 gcgcccgctg ttcaatccgc tcatcctcca tatcggtcgc aaagaacagg tgcatgaact
     1921 ggcatatctt tctgccggac ctctggatag gctggcagat gcctgctggc ccggaccact
     1981 tacactgttg ctacctaaaa aagagaccgt gcatgatctg atcaccgcgg gcagccccct
     2041 ggtagctgtg cgcatgccag accatcccct tacactcccc ctgctgcaaa gactgcattt
     2101 cccgctggct gcacccagcg ccaatttgtt cggtactgtg agccctacca gtcctgtaca
     2161 tgttgcagaa cagctggatg gaaagatacc aatgatactg gatggcggac cctgtcgggt
     2221 aggtctggaa tcaaccatcg tcaaatggga aaatgacacc ctgcacatcc tgcgcacagg
     2281 tgccattact ccggagatca tagaaaatgt aaccggctta gttccgttca tcagcaaaga
     2341 gatctcagaa aaacccgaag ccccggggca actgaaaagc cattacgcac ctgaaacccc
     2401 gttgcaatgc ggagatcttg ttcaactgct ggctgatagt ccgtcgcaaa agatagcagt
     2461 gctctgtttc aggcaggtgc ccgatgattc ccgtatttgt gcccatttta ccctgtctgc
     2521 agcaggtagt gagaaagaag ctgctaccag gctttttgaa ggccttcgca ccctcgataa
     2581 atcaggtgcc gattgcatcg tggcagagct gctgcctgaa aaagggctcg gacttgccgt
     2641 gaatgatcgt ttacgaagag ccgcaaccgt atcctgatta tttattattt ttgagaacca
     2701 aaatcatgtc tatgcgctat ttacgccacg ccggacttct cgtgatcaca ctgaccattt
     2761 ctgtactctc ttttgctcag gctgaaaagt atcagggcac tatgtttgaa aacatggctc
     2821 cagcctcttt tgaatgggct gccaacagac tggctaccct tgatcagctg gtaaccctta
     2881 cagacgccca gaaagaagaa gtggaggtca tcaatatgcg gtacgcctac aggatggatg
     2941 cactggccag acaaaccatg gatccagaaa cactgaaagc acacagagaa ggtctgtttg
     3001 actaccggat ggaagattac aaacgcattc ttaacccgga gcaggtggcc attctggtag
     3061 ctcatgaaga ggatctgaaa gaagataacc tgctgtatta atcctccact ttttgccctt
     3121 ttcagaccgg cagatctgtc ttattgcaga tttgtggcac ggtttttata cctgtattcg
     3181 taaccattaa ccaataaata aatcaagatg aaaaaatcaa ccctcgtact tgccttcatg
     3241 gcaatggccg cgttcggttc tgtccaggca caggatatgt ctcgcggtga tgctgacaaa
     3301 cagcagcaga agaaagagaa tatgtcgcag gaagaccgcg accgtatgcg tgaagaacgt
     3361 caggacaaaa aagacaaggt caacagcaat atgtctgatg acgcacaagg ccgtgccaaa
     3421 gacatgaccg acaaaatgga tgagatcctg gatctgacag acgcacaata ccgccaggct
     3481 atggaagtga acctgcgtta ttacaaccag ctggatcaga tgcgtgctcg ttacgccgga
     3541 caggaagatg ccaataaaga gcagatgaaa gcaaagaaag atgaactttc ccgcgaacgt
     3601 ctgaactcat acaaatcatt cctgacctca gagcagtggt caaaatttga agagcgccgt
     3661 ggcgatgcca aagaagagca cgagtcaaag atgaccaagg aagaaaagaa agagcgcatg
     3721 gaaaacatga ctcctgaaga aaaagagcgc atgaaagagc gcaaggagca gaagagcagc
     3781 accggtacca gcactccccg caacaaagga gatatggaca aggaaaagat gcaggagaag
     3841 cgtggcagca gcagcagcaa aagcagcggc agcgataact gatcattaca tcaagacact
     3901 aaaaagcccg gttctccggg cttttttttt attccttttt tagccaggca cctttgatgc
     3961 ccgtcgtttc tgcaaaactc tgcctgtaag cataccggtc tgtgccctgt ggtacaggca
     4021 ccactaccat atgcagatcg ccggatacct gaacagcagc ttcgaagccg ctgtttcgca
     4081 atctttcctg ctggttttca gcatttgcct gttccttaaa tgcaccgcaa accaaccaga
     4141 aaccgctacc cgtaacaacc tgttcaacag gctcagcaat gaccagaact gccgttgtcg
     4201 gttcgttcgt ttctctcgtg gaggatacag aactgatagc aggcatttgc tcagctgcat
     4261 ataacaaggc tatttgctca ttctgctgtg cccggatact gtcaaacaaa ttagccaact
     4321 ggtgtgaacc gcttcctccg ctgttctgca taggcagaaa taacaaagcc agcaccacac
     4381 aggccgctgc aagccatcct aaaaccgatg agatgcgacg ttcttttcgg gcagttacgg
     4441 gtatttccgg tggtgctgtg accatagcct tcttcctgat gggggaagcc tgaaaagcgg
     4501 gtaacccaaa agcacttggc tcatacgatg cggtaggatc tgcggtaaag cgcatacttc
     4561 cttccgggtc tctgaccagc ctgccaatac ccggaaactg aatcgtctct cctgcctgca
     4621 gcaattccag ctgtgacctg gtgtatgctg ttaccatacg ctctgcttcc attgaatgga
     4681 tctgctgacg catagcaacc ctggcaacga gtaagccatc gttctcacgc aaacgctcat
     4741 taaatacgaa cgtagttccg ggcggcgtaa aatggaatga ggtaggatgc atcctggccg
     4801 gcctgtagcg cttcataaaa ccgcccagac cgggaatgac cacgtagtca tgatcctgac
     4861 aaagctcctg aatatagacc ggtatcaact gcatcagacg aacgtccaaa tgtagtgaat
     4921 tccgaaaaat cctgtatcag aaagagaacg aaagaccacc cagcatgttg aaaccatagc
     4981 ttgggtagtt cagatatcgt tcgtatttga ttcctgcaat gttattgacc tccaggaaaa
     5041 tgttgaaaag attgttgtat ctgtacacgg ccgacaggtt c
//
LOCUS       DAOMAX010000194         5058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2822_length_5058_cov_3.997002, whole genome shotgun sequence.
ACCESSION   DAOMAX010000194 DAOMAX010000000
VERSION     DAOMAX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_5058_cov_3.997002"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK742_09755"
     CDS             <1..629
                     /locus_tag="PK742_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU76914.1"
                     /translation="LRVRYDAYLIDLHMPGRDGVSVAHAIRTKHPDARIIALTADVVT
                     PVAELRQDGLFNDVLHKPYKEQDLLDMLAGNSSAEQKPQEEANQLSMQQHYSLDEIKM
                     FCGSDQKMLWEIVTTFLESAPEQISMLQEALENKDDEGVRNAAHKMLPAFQHFHMQEA
                     VPLLKDLELLGLQANHARPDQYVHAVRQAALQVFRELKREMEMAGVFE"
     gene            complement(631..2004)
                     /locus_tag="PK742_09760"
     CDS             complement(631..2004)
                     /locus_tag="PK742_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQU76915.1"
                     /translation="MPHRVLVIDDDPTFCRMLEGFLQRKGMDAKGVFSGEEALLTMEK
                     ETFDIILSDYRLPEKDGIELLDEIREHHGPVPFIIMTSYADIRIAVRAMKLGAYDYVT
                     KPVNPDEILLSIKGAMERNTATIIQQEQVVVPVKVSNAENGYLTGESDISKRLQEHIK
                     LVAPTNISVLIEGESGSGKEYVAKRIHEQSNRSARPFVSIDCGALSKELAASELFGHI
                     KGSFTGAIQDKTGQFEAANGGTLFLDEIGNLSYDIQVQMLRALQERRIRKIGSNHDIS
                     VDVRILAATNEDLLDAVGRGDFREDLYHRLNEFKIQVSPLRERPEDIPMFCAHFLQQA
                     NQELNKQLAGFAQDVMDIFLKYSWPGNLREMRNVIRRSALLSTGDRVGKDALPPEMIQ
                     ESMGISSRRDLTEQASDLRSVAGKTERDMIIQTLVKVNYNKSKAAKLLKIDRKTLYNK
                     MKQYEIE"
     gene            2190..>5058
                     /locus_tag="PK742_09765"
     CDS             2190..>5058
                     /locus_tag="PK742_09765"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HQU76916.1"
                     /translation="MNRFLLSRVLMSCLSAMLFLLAANTASAQYCDVSYGLGTSDGDY
                     CDRFVLEGIDNTSGAGAVYNDYTAFSTLLNPGLTYEFTIYNTPEWTEYYAVWVDWNQN
                     ETFEDDENLIGLGIDNYVDIIAGGNATISFTVPVTALPGTTRLRLVCAYFPDVPMIAC
                     PDYDGGDLDYYYGEVEDYSIVIPASGPYDLAAVDLPGLVSGCGLGLQDITLEVVNVGT
                     DPAGGFSLSYQVTDPVLGVLPAVTEDYTGADLASFESLSYTFATPADLSNSGDYTIDG
                     WITWDLDGLDANNNTNESFTSIPSISSFPYTENFEGGSGGWTSGGVNSSWELGYPSAS
                     IIDGPPPGTPSSENSWATGLYDFYNLSEDSWVLSPCFDFSSLVVPYVKFDIWWEVYEF
                     WDGARLEYSLDAGTTWQPIGDIGTGDNWYASGGCYSFGFDPFTGIYYPAWEGSGGGWK
                     TAEHDLSFLAGEPQVQLRMHFKSYVFSGFQDGIAFDNFFVGDPYNNDIGVTALVDPSS
                     GPSLGSTETVTVQLENFGLFDQSGFDVAYQLDGGTVVTETFAGTLPAGETAEFTFSTT
                     EDLSADGDYTFVAWTELGTDEDLSNDTLTQVVSNLEPITGTDAYYIYSNVYGGPEPWF
                     TTSSSESMDAVFGVDEWTLGFFETIDVDAVFSTATCFVWLEGGDAMANELETFLNSNY
                     VAIQNWVASGGHLFLNSAPNEGDGMDYYFGDVSLFYAWYTNTAAPGDPGHPIFDGPFT
                     PVGASWSGSSFGHAVVEGDADPVIIDAFDDSRVVLAEKHWGAGIAMFGGMTPTYFHSP
                     ATEATNLRNNILSYLGSCILSDIDLGLASVIAPVNGCGLGDEAVTVKIRNYGAAPQSN
                     VPVNYQLDGGVVVSETWIGTLDPGETIEYTFASLADVSALGDHDIVAWTSFPGDTILG
                     NDTADATITNIPVIDVYPYLEAFEAGSGGWTSGGTNSTWAQG"
BASE COUNT         1223 a         1236 c         1171 g         1428 t
ORIGIN      
        1 gccttcgtgt gcgttatgat gcctatctga tcgatcttca tatgccgggt cgggatggtg
       61 tatctgtagc acatgccatt cgcaccaaac accctgatgc caggatcatt gcactcactg
      121 cagatgtggt aacacccgtt gcagagctca ggcaagatgg cctgttcaat gatgtactgc
      181 ataaaccata taaagagcag gatctgctgg atatgcttgc cggcaacagt tcagcagagc
      241 agaaaccaca ggaagaggca aatcaactat ccatgcagca gcactattct cttgatgaga
      301 tcaaaatgtt ttgcggaagt gaccagaaaa tgctttggga gatcgttaca acatttctgg
      361 aaagcgctcc tgaacagatc agcatgctgc aggaggcact ggagaacaaa gatgacgaag
      421 gggtcagaaa tgctgcgcac aagatgctgc ctgcttttca gcattttcat atgcaggagg
      481 ctgttccatt attgaaagat ctggagctgc tgggattgca agccaaccat gccagacctg
      541 atcaatacgt gcatgctgta cgccaggcgg ctcttcaggt atttcgggag cttaaaagag
      601 agatggagat ggcaggtgta tttgaatgat ctactcgatc tcgtattgct tcattttatt
      661 atacaaagtc tttctgtcta tcttcagcaa cttagctgct ttgcttttat tgtagttcac
      721 cttgaccaac gtctggatga tcatatctct ttctgtcttt ccggcaacag accgaagatc
      781 acttgcctgt tcagtcaggt ctcgtcgcga actgatgccc atactttcct ggatcatctc
      841 aggtggtaaa gcgtcctttc ctacccgatc tcctgttgat aataaagctg agcgcctgat
      901 cacattgcgc atttcacgca ggttgcccgg ccaggaatat ttcaaaaaaa tatccatcac
      961 atcctgagcg aaacctgcca gttgcttatt cagttcctgg ttggcctgtt gcagaaaatg
     1021 cgcacaaaac atgggtatat cttccggtcg ttcacgcaag ggtgaaacct gtatcttgaa
     1081 ttcattcaac cggtgataca ggtcttcccg gaaatctccc cgtccaacag catccagaag
     1141 gtcttcattg gtagctgcca gaatgcgcac atctacagaa atatcatggt tgctgcctat
     1201 cttcctgata cgcctctcct gtaacgcccg caacatctgc acctggatat catatgacag
     1261 atttcctatt tcatccagga ataaggttcc accgttggct gcttcaaact gtccggtctt
     1321 atcctgaatg gcaccggtaa aggagccctt tatatgcccg aacaattcac tggctgcaag
     1381 ctctttactc agagccccgc aatcaatact gacaaatggc ctggcacttc tgttgctctg
     1441 ttcatgtatg cgtttggcaa catattcttt gccactgccg ctttctcctt caatcaaaac
     1501 ggagatattg gttggcgcca ccagtttgat atgttcctgc aaacgtttgc tgatgtcgct
     1561 ttcaccagtc agataaccat tttcagcatt gctgaccttt acaggcacca ccacctgctc
     1621 ctgctgtata atggttgcgg tattcctttc catggcacct tttatactca gcagaatttc
     1681 atccggattg acaggctttg taacataatc atatgccccg agtttcatgg ctctgacagc
     1741 aatacgtata tctgcataag atgtcatgat gatgaacggc accgggccat ggtgttctct
     1801 gatctcatca agcaattcaa taccatcttt ttcaggcagg cggtaatcgc tcaaaataat
     1861 atcaaaggtt tctttttcca tggtgagcaa agcttcctca cctgaaaaaa cgcctttggc
     1921 atccatgcct tttctctgca gaaatccctc cagcatccgg cagaatgtcg gatcatcatc
     1981 aataactaat actcgatggg gcatatgggt cggttcgatt gtaaaaatac aattcctttc
     2041 ctcccgacag gaatggaggc tttaacacag gcttcccggg catataaatt attgcttatc
     2101 tgcactttat gttttaccta aaatattgat ttgcaaatct caaacctttc gtacttttag
     2161 tgaaaatctc atctaaaaca tctgaatcta tgaaccgatt tctactctca agagtgctga
     2221 tgagttgttt gtctgctatg ctattcctgc tggcagccaa cactgcatct gcacagtatt
     2281 gtgatgtgag ttatggctta ggtacaagtg acggcgatta ttgtgatcgt tttgtactgg
     2341 aaggcattga caacacatcc ggagccggag ccgtgtacaa cgactacaca gccttctcca
     2401 ctttattgaa ccccggactt acgtatgagt tcaccattta taatactcct gaatggacag
     2461 agtattatgc ggtttgggtt gactggaatc aaaacgaaac gtttgaagat gacgaaaacc
     2521 tgatcggcct gggtattgac aactatgttg acatcattgc aggtggaaat gctaccattt
     2581 cctttaccgt tccggttacc gcacttcccg gcaccaccag actgcgcctc gtttgtgcgt
     2641 atttccctga tgtaccgatg attgcctgcc ctgattatga cggaggtgat ctggactact
     2701 attatggaga ggtagaagac tattctatcg tgattcctgc ttcaggtccg tacgacctgg
     2761 ctgctgtaga tcttccgggt ctggtatcag gatgcggact aggtttacaa gacatcactc
     2821 ttgaggtcgt aaacgtagga acagaccctg caggaggttt cagtttgtcc tatcaggtta
     2881 ccgaccctgt tcttggtgtt ctacctgcag taactgaaga ctatacagga gcagatctgg
     2941 cttcatttga gtcattgtct tacacatttg caactcccgc tgatctcagt aattccggtg
     3001 actataccat tgatggctgg atcacatggg atcttgatgg tttggatgcc aacaacaata
     3061 ccaatgaatc atttacttcc attccttcta tcagctcctt cccttacaca gagaactttg
     3121 aaggcggttc cggtggttgg acaagtggtg gagtaaacag ttcctgggag cttggttatc
     3181 cttctgcttc catcattgat ggcccccctc cgggtactcc ctcttcagag aattcctggg
     3241 caaccggctt atatgatttt tataacctga gcgaagacag ctgggtactg agtccctgtt
     3301 ttgacttcag cagtctcgtg gttccctatg tcaaattcga catctggtgg gaagtatatg
     3361 agttctggga tggagcccgc ctggaatatt cattggatgc aggtacaacc tggcaaccta
     3421 tcggagatat tggaaccggc gataactggt atgcatccgg tggttgctac tccttcggat
     3481 ttgatccttt cacaggtatc tactacccag cctgggaagg cagcggaggt ggctggaaaa
     3541 cagctgaaca tgacctgagc ttcctggcag gtgaaccaca ggtacaactg cgtatgcact
     3601 tcaagtcata tgtattttca ggattccagg acggtatcgc ttttgacaac ttctttgtag
     3661 gcgatccata taacaatgat atcggtgtaa ccgcattggt cgatccttca tccggcccgt
     3721 cgctcggctc aactgaaact gttaccgtgc aactggaaaa cttcggactg ttcgatcagt
     3781 caggttttga tgtagcctat cagctggatg gtggtactgt ggtaaccgaa acgtttgccg
     3841 gcacactgcc tgccggagaa actgcagaat ttaccttctc cacaacagaa gatctgagtg
     3901 cagatggcga ctataccttc gttgcctgga cagaattggg tactgatgaa gatctgagta
     3961 atgatacact tactcaggtt gtatccaacc tcgaaccgat cactggcacc gatgcatatt
     4021 atatttattc caacgtgtat ggcgggcctg aaccctggtt caccacttcc agctctgagt
     4081 ctatggacgc agtgtttggt gtggatgaat ggaccctcgg cttctttgaa accatagatg
     4141 tggatgccgt attctctact gccacctgtt tcgtctggct ggagggtggt gacgctatgg
     4201 ccaatgaact ggaaacattc ctcaacagca actatgtggc cattcaaaac tgggttgcgt
     4261 caggcggtca ccttttcctg aactcagccc cgaacgaagg agatggcatg gattattatt
     4321 ttggagatgt ttctcttttc tatgcctggt acactaatac tgctgctccc ggcgatcccg
     4381 gccacccgat cttcgacggc ccgttcactc cggtgggtgc ctcatggtcc ggttcttctt
     4441 tcggtcatgc agtagtagag ggtgatgcag atcctgtgat catagatgcc tttgacgaca
     4501 gccgagtagt actggcagaa aaacactggg gtgcaggtat cgccatgttt ggtggcatga
     4561 ccccaactta tttccactcg ccggctacag aagctaccaa ccttagaaac aacatcctct
     4621 cctacctggg ttcctgtatc ctgagtgata tcgacctcgg tctggcctct gttattgctc
     4681 cggtaaatgg ttgcggcctg ggtgatgaag ccgttaccgt gaagatccgc aactacggcg
     4741 ctgctccgca aagcaacgtt ccggtcaact accagctgga tggcggtgtt gtggtttctg
     4801 aaacatggat aggtacactg gatcccggtg aaacaataga atatacattt gcctccctgg
     4861 cagatgtcag tgctttgggc gatcatgata tcgttgcatg gacctctttc ccgggagata
     4921 ctattttagg taatgatacc gctgatgcaa ccatcaccaa tattccggtc attgatgtgt
     4981 atccttacct cgaagcattt gaggcaggtt caggcggatg gacctcaggt ggaaccaact
     5041 ctacctgggc tcagggaa
//
LOCUS       DAOMAX010000195         5047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2833_length_5047_cov_3.840144, whole genome shotgun sequence.
ACCESSION   DAOMAX010000195 DAOMAX010000000
VERSION     DAOMAX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2833_length_5047_cov_3.840144"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2222)
                     /locus_tag="PK742_09770"
     CDS             complement(<1..2222)
                     /locus_tag="PK742_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76917.1"
                     /translation="MKQLLIIVLISCFTATYAQWPTVSIHDLQFRDEASLVAEDDLSL
                     YDGDTVMVQGIVIFDPCTYALSTSGSRIGTFLVDETETGEWTGLHVLIDPGASGYGGT
                     LEELNDATLFIDNFQVGNVVECAGIIGTFDENTQIVLIDEPSSIIGFSSVPDPEVLGI
                     DVFSTSDGGGGQIQETVTGEAYEGVYVQFNNVFVVDVAPFGDFRWTWYLQDADGNKIQ
                     IRDASGFYRNDTNTDDECSIWSGGAAGETATPDEFTPPTTGTYLEYVRGVIMEAYGGT
                     AYSLAPLTPGDVGPELASPPSVTNINRDPYVPTSTESVTVTATITDIDGTVAGASLNY
                     SYGYGSTAWSSADMINTAGDTWEASIPGPGVDGEYVNFYIYAEDADGNSIETPGSVSP
                     VIYQVFDDGIQHIVQIQYTPAASGTSIWVSDHAFNMDIEAVVMASRQTYDLGWLIVQD
                     DDSPWSGIRIYSQPGDGTTELLRGDKIRITSAMVREDFGATLLDSVTITVIGTEDLYE
                     PVTGLDPESINAQVFDQAEAYESMFIRFEDVYTTSNNPDAPGGPFGEWRFNTDNVAGT
                     GLRADDLSYEVPFEFAADSVTVGEPMDWIQGILTYSFSNFKLEPRDLNDIAGFSTEYP
                     NKITAFSFDGLGATGTIDQDAQTITVEVPSGTDVSSLSPTVTITGVTLDPPSGTTQDF
                     SSPVTYTCYAPVSGTGRAYEVTVTMVDALSDLIDNQYLVVYPNPVSDNMTVQFTAKES
                     G"
     gene            complement(2306..2911)
                     /locus_tag="PK742_09775"
     CDS             complement(2306..2911)
                     /locus_tag="PK742_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76918.1"
                     /translation="MRTLFFTGCLLFTLGVSAQSNSFGHQRRGHVNPFRINYFSYDIN
                     HPGVQLGPELDFLWTRTEKIRCESGMKVIDKQLLFVPNFGFILYPESIVNLYAGLELD
                     YRVIYDRGIIFDIFFGGAYCQRFGSDKLPLGEQDFETVIMDSRSAFLPTFGMGTGYDF
                     HKRVFGGPPIIISLRAVNASLSPSTDFYNPCIQLGCTIGVQ"
     gene            complement(3099..4772)
                     /locus_tag="PK742_09780"
     CDS             complement(3099..4772)
                     /locus_tag="PK742_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HQU76919.1"
                     /translation="MRKGKYLLLMLFTAQMAVAQQELKVTMPVENATVYLSGAQLFHS
                     KSVSLPKGQTDVIVEGVSNNLDASSLQAGGVGNFTILDVQYRLYYPEPVGGTELPDAI
                     QKKINKLNDSIMDLDYTTQAINNRKEVLNVQRNLLYNNKLLKPDNANDSLELIRNTIE
                     YYNQKLSEIATEQLDLDKRIHALQKERSEMLVRLNELNNYWAQENAKRSNAPVPQVII
                     SLITEAAGSATLDLNYLSYSAGWYATYDIKATDIGQPVRMTYKANVWQNSGIDWKDVL
                     LTFSTGNPTIGNNPPQLTTWYLTYYNYYYNEKKAMDRTDDVELDMVTPSMAEDLYLGN
                     ASVGTGYANAGQAYDHTSQQQTLTNTEFEVKLKYSIPNDGKSHLVALQDKSLKADFNY
                     LIVPKLDNDAFLIARITGWEDGGLMPGVANVYYKNSLVGKTSLNTLTLNDTLSVSMGR
                     DKGIDVERKVLKEKSSDKLIGSDRRVERMYEITVRNSKAIGIEIVVMDQIPISNDQTI
                     KVEVGEYGKGELDEYTGFITWRDKLGSKKTGTYKFGYTVTYPKDRPLQL"
BASE COUNT         1200 a         1245 c         1303 g         1299 t
ORIGIN      
        1 cctgactctt tggcagtaaa ttgaactgtc atattgtctg aaaccggatt gggatagacc
       61 actaaatatt ggttatcaat caggtctgac aaggcatcta ccatagttac ggtaacctca
      121 taagccctgc cggttccgga aacaggcgca tagcaggtat aggtaacggg cgaactgaaa
      181 tcctgggtgg tacctgaagg cggatcaagg gtaacaccgg ttatggtcac tgtgggcgac
      241 agggaagata cgtctgttcc ggacggaact tccacggtga tggtctgagc atcctggtca
      301 atggtaccgg tagcgcccag tccgtcaaaa ctgaatgcag tgatcttatt gggatactct
      361 gtactgaagc cggcaatatc attcagatca cggggctcca gtttgaaatt gctgaagctg
      421 taggtcagaa taccctggat ccagtccatc ggttctccga cggtcacact atctgctgca
      481 aactcgaacg gcacttcgta agacagatcg tcggcccgga gtcctgttcc agcaacattg
      541 tctgtattga aacgccattc gccaaaaggc ccgccgggtg catccgggtt attgctggta
      601 gtatagacgt cttcaaaacg gatgaacata ctttcatagg cttcggcctg gtcaaatacc
      661 tgggcattga tggattcagg gtcaaggccg gttaccggtt catacaggtc ttcagtgcct
      721 attaccgtga tggtcactga gtcgagcagg gtagccccaa aatcttcccg aaccatggca
      781 gaagtaatgc ggatcttatc tccacgcaac aattctgttg tgccatcacc aggctgagaa
      841 tagatgcgga taccactcca ggggctgtca tcatcctgga cgatcagcca acccaggtcg
      901 taggtctggc gacttgccat cacaacagct tcgatgtcca tgttgaaggc atggtcactc
      961 acccagatgg aagtaccgga agcagcgggt gtgtactgga tctgcactat gtgctggata
     1021 ccgtcatcga acacctggta gataacagga gatacactgc ccggggtttc tatactgttg
     1081 ccatctgcat cttcagcata gatatagaag tttacatact caccatccac acccggacca
     1141 ggaatgctgg cttcccaggt atcgcctgcc gtattgatca tatcagcaga tgaccaggct
     1201 gtactgccat acccgtagga gtaattcagg gaagcaccgg ccaccgttcc gtcaatgtca
     1261 gtaatggtag cagtgacagt tacactttcg gttgaagttg gcacataagg atcgcggttg
     1321 atattggtaa cagaaggcgg tgatgccagt tccggaccta catcgccggg agtgagcgga
     1381 gccagcgagt aggctgtacc gccgtaagct tccatgatga ccccacgaac atattcgagg
     1441 taggttccgg tagtaggggg tgtaaattca tcaggggtag ctgtttcgcc ggctgcacca
     1501 ccactccata tggagcattc atcgtcggta ttggtatcgt tccggtagaa tccggaagca
     1561 tctctgatct ggatcttgtt accatcggca tcctgcagat accaggtcca tcggaaatcg
     1621 ccgaagggtg ccacatccac cacaaagacg ttattgaact gcacataaac tccttcataa
     1681 gcttctccgg tcacggtctc ctggatctgt ccgccaccac catcgctggt ggagaagaca
     1741 tcaataccca gcacttccgg gtctggtact gatgaaaaac cgatgattga cgatggctca
     1801 tctatcagca cgatctgggt gttctcatca aaagttccta tgattccggc acattccacc
     1861 acattaccca cctggaaatt gtcgatgaac aaagtggcat cgttgagttc ttccagggtt
     1921 ccgccatagc cggaagcacc ggggtcaata agcacatgca ggccggtcca ctcgccggtt
     1981 tcggtctcgt caaccagaaa agtgcctatt cggctgcctg aggtggacaa cgcataggta
     2041 caggggtcaa aaatgacgat gccttgtacc atgaccgtat caccgtcata gagtgacaga
     2101 tcgtcttctg caacaaggct ggcttcatca cgaaactgta aatcgtggat gctgaccgtt
     2161 ggccattgtg cgtaagttgc tgtaaaacaa gagattaata caataatgag taattgcttc
     2221 atgatccttt aagctttgca gcaaaggtaa aaagccactt tgaagtgttt cgtactgatt
     2281 acgttaaaac tatattaacc cacatttact gcacaccaat ggtgcacccc agctggatac
     2341 agggattgta aaagtcggtg gaagggctca gactggcgtt tacagcccgc agagaaatga
     2401 taatgggcgg cccgccaaag acccttttgt ggaaatcgta tcctgttccc attccgaaag
     2461 tgggcagaaa agcgctgcga ctgtccatga tcacggtctc aaaatcctgt tctccaagtg
     2521 gcagcttatc actgccaaag cgctgacagt aggctccgcc gaaaaagatg tcaaagatga
     2581 tgcctctgtc atagatgacc cggtagtcca gttccagacc ggcatacagg ttcacaatgg
     2641 attccggata caggatgaat ccaaaattgg gaacgaacag caactgcttg tcaatgacct
     2701 tcatgccgct ttcgcaacgg atcttttcgg tgcgggtcca cagaaaatcg agctccgggc
     2761 ctaactgcac acccggatgg ttgatatcgt aagagaaata gttgatgcgg aaaggattga
     2821 catgcccgcg acgctggtgc ccgaaggaat tgctttgagc cgataccccc agggtgaaca
     2881 acaaacatcc ggtaaagaaa agggtgcgca tatcggcaca aatttacgga atcaggcgga
     2941 gagggtttgt gaataagacg ttaagaccag cgtcaatgtt tggtatgatg tgacaattta
     3001 caccgattcc aattgggagg tagtgagtga gtgaggcgtg agcagttggt caaaaaaaac
     3061 gccgcccttc cgggcggcgc tgctttgaat cagacgtctt acaactgcaa gggacgatct
     3121 ttcggatagg tcacggtata accgaactta taggtacctg tctttttgct tcccagtttg
     3181 tctctccagg tgatgaagcc ggtgtattca tccagttcac ctttgccgta ctcacccact
     3241 tccactttga tggtctggtc gttggagatc ggtatctgat ccatcactac gatctcgatt
     3301 ccgatggcct tgctgttccg cacggtgatc tcatacatgc gctctaccct tctgtctgag
     3361 ccgatgagct tatcgctgga tttttctttc agcactttgc gttctacatc aatgcccttg
     3421 tcgcgaccca tgctcacact cagtgtatcg ttgagggtca gggtgttcag gcttgtcttg
     3481 cccaccaggc tgttcttgta atagacattg gccacacccg gcatcaggcc gccatcttcc
     3541 catcctgtaa tacgggctat caggaaggcg tcgttgtcca gtttcggcac gatgaggtag
     3601 ttgaaatcag ctttgagcga tttatcctgc agggccacca ggtgactctt tccatcgttg
     3661 ggaatgctgt acttcagttt cacctcgaat tccgtattcg tcagggtctg ttgctgagag
     3721 gtatggtcat atgcctgacc ggcgttcgca tatcctgtgc ctacactggc attacccaga
     3781 tacagatctt ctgccatgga aggtgtcacc atatcaagct ccacatcgtc agttctgtcc
     3841 atggctttct tctcattgta gtagtagttg taataggtca ggtaccaggt ggtcagctgt
     3901 ggcgggttat tgccgatggt agggttgcct gtactgaagg tgagcaatac atctttccag
     3961 tcgataccgc tgttctgcca tacgttggcc ttataggtca tgcgcaccgg ctggccgatg
     4021 tcggtagcct tgatatcata agtagcatac cagcccgcac tgtagctcag gtagttgaga
     4081 tccagcgtag cggaacccgc tgcttcggta atgagcgata tgatgacctg gggcaccggt
     4141 gcattgcttc ttttcgcatt ttcctgggcc cagtagttgt tcagctcatt cagacgtacc
     4201 agcatctctg acctttcttt ctggagtgca tggatgcgct tgtccaggtc cagctgctcg
     4261 gtagcgatct cactcagctt ctggttgtaa tattcaatgg tattccggat gagctccagg
     4321 ctgtcattgg cattatcagg tttgagcagc ttgttgttgt acagcaagtt gcgctggaca
     4381 ttcagcacct ctttcctgtt gttgatggcc tgggtggtat agtccaggtc catgatggag
     4441 tcattgagtt tgttgatctt tttctggatg gcatccggca gttccgttcc acccactggt
     4501 tccggatagt acaggcggta ctgcacgtcc aggatggtga agttaccaac accgcccgcc
     4561 tggaggctgg aggcatcgag gttattggag acgccctcca cgatcacgtc cgtctggcct
     4621 ttgggcaggc tgaccgactt gctgtggaag agttgggcgc cgctcaggta cacggttgcg
     4681 ttttcgaccg gcatggtgac cttcagttcc tgctgcgcca cggccatttg tgctgtgaac
     4741 agcatgagga gtagatattt cccttttcgc atacattgct tttttcaggt agatgcgggg
     4801 ggaaggggga ttccataagg attttaacca cattttatct gccagctgat cgatcgatca
     4861 acacaataga tcgtatcatc tatctttatt gccgttgaag gacctaggct ccaaacttgt
     4921 gatcaggttg ttcttaaaat acctctatga agaaagaata ctgggacgta accgatgagc
     4981 aggtaatgga aaagactggt aaagagatag cagattggat aaagatcctg gatctgtttg
     5041 gtgccgc
//
LOCUS       DAOMAX010000196         5043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2838_length_5043_cov_4.383721, whole genome shotgun sequence.
ACCESSION   DAOMAX010000196 DAOMAX010000000
VERSION     DAOMAX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_5043_cov_4.383721"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PK742_09785"
     CDS             complement(<1..441)
                     /locus_tag="PK742_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76920.1"
                     /translation="MKTKATVSAIFHSSLERAFKTPILGDVTKFLVGYGPVPAVVKIT
                     DDATWGKPGGTRIPHGAKNFASNGGPIGIDQVYVREENTYWEWGVATFLQPSMGFTEF
                     RGQMSFTEQAPEQIRVEWTYTLYSRFVLAYPFHWFFTKVFWRGQM"
     gene            complement(451..975)
                     /locus_tag="PK742_09790"
     CDS             complement(451..975)
                     /locus_tag="PK742_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HQU76921.1"
                     /translation="MLLSTLQKRFEKHMHRHPGMQWKDVQARLEANPDKLISLNEMDI
                     TDGEPDVVGQETDTGAFIFMDCAAESPKGRRSLCYDPDALESRKEHKPKNSAIGMAAE
                     MGIELLNEEQYAWLQKLGPFDTKTSSWVKTPEDVRKLGGAIFCDHRFGRIFTYHNGAE
                     SYYAARGFRGMILI"
     gene            complement(1011..1310)
                     /locus_tag="PK742_09795"
     CDS             complement(1011..1310)
                     /locus_tag="PK742_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76922.1"
                     /translation="MINNIYSLELNTEKPAVQVLHRSDKVQVTAIGLLRGQLLKKHLT
                     SNPAMICVVEGTLDFQTETESISLRSGDTMDIPVDIPHEVTGMDDRNVFLLIRES"
     gene            complement(1357..2001)
                     /locus_tag="PK742_09800"
     CDS             complement(1357..2001)
                     /locus_tag="PK742_09800"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQU76923.1"
                     /translation="MYDLSYFKEPEHAVVLQFMQEHPFAFLTGSFADGRQAATQVPVM
                     LIPQEDRLVIQGHIMRKTDHHKAFMENPNALLVFTGPSTYVSASWYTNPQIGSTWNYM
                     SVHAQGKIRFMDDDELIDLMKRLTLHFEKGNPSSPTIYDNLPDTFLEHMMPAIVGFEI
                     KVDTLENVFKLSQNRDEESYDNIITRLEEQGGDAAYIANEMRKRRTRLFPDTSS"
     gene            complement(2057..3472)
                     /locus_tag="PK742_09805"
     CDS             complement(2057..3472)
                     /locus_tag="PK742_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76924.1"
                     /translation="MKLFKVLPFIIGFICPLYLAAQTFQQNDEKPIRLQFPLIEYPYL
                     SYASQTVSAPDDPVFTDYLKMFANPSMSQSLALSNDLYLTTHYGIKRLFHEQDTFKRI
                     LFSSLTAAGADLLLLYAPLGNGWLHEEYHRSVMTLFYINSFNDMNTFPIGKRTVSVNS
                     IDDAELASMKLHSNPDFVRLMSAGIEAQYDQIQEMQKTNFYNDQDLPNLSLYWISTVN
                     SIFYVNSCADSSANQLTDEIMLEEGSNTEVRDFTGLDLAAWTYDLFNPDEPYAERGIH
                     PSGVGIKRYIKPADLTQEQLDYLKKQGGLQFLNLLSPALYSFYRIRLRSDSSGTYYGN
                     FAIRHILTSFGSDISTNIMLQTPLRNFFFAYHLYQNYNHSFHGLEIEVIDEQIVVKNK
                     TLLLSPGLMIWSQPEDQSFTTSKSAIGGLATLTANYPITRALYFQLSMTAKTPGWAMG
                     NMYLDANYTVSSGIALQFGND"
     gene            complement(3469..4533)
                     /locus_tag="PK742_09810"
     CDS             complement(3469..4533)
                     /locus_tag="PK742_09810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU76925.1"
                     /translation="MHLKHIYWFAYYNEDEPSVRYRCIYPLRYLAETHDITYSIVHPG
                     YDRKHIAAFIRIYLSALIFRKKNSLIVFQKIFTGGIYAHALKLLLFFRPTFTLYDTDD
                     AEHTRRTTDTIHHFMKHCSACSAGSRSLIQYISPLNPNAFLLTSPVIDHGVIKREREN
                     IFTIGWIGYYGAHLESLETLLFPALTQIPFPVRFIVLGARNEGEAEHIISSFKKYDHI
                     SVEAPLNISWLDERSVYHQIARFDVGVSPLLDTEFNRGKSAFKLKQCLSCGIPVLGSS
                     VGENKYFLQEGVNGYFCDSPKEYLERIIAIRNAPLSDYMTMSKEAAASSRLFSMEHFS
                     ASLLAITENLPIDHPLNQTL"
     gene            complement(4543..>5043)
                     /locus_tag="PK742_09815"
     CDS             complement(4543..>5043)
                     /locus_tag="PK742_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76926.1"
                     /translation="KGLDYYLGMGGQSLPSGRASDNKVYCAGLGEYYIHEVFHAETDS
                     YFPDMHFWAAEGIATFLGGSRGKSLDWHIERTDRYLQSHPETDLNEMLKLTNLDNETS
                     FHYVLGGLIAEMIFEKGGWPLLLELMSGGVHDEDYYHTIEVLLGISKQELNEQIRQEL
                     HKRSKD"
BASE COUNT         1274 a         1113 c         1209 g         1447 t
ORIGIN      
        1 catttgtcca cgccagaata ccttggtgaa aaaccagtga aaaggatagg ccaatacaaa
       61 gcgggaatac aaggtatagg tccactccac ccgtatctgt tccggtgcct gttcagtaaa
      121 ggacatctgt ccgcgaaact ccgtgaaacc catgctgggc tgcaggaaag ttgccacccc
      181 ccattcccag tatgtattct cttcccgaac gtacacctgg tcaatgccga tggggccgcc
      241 attggaggcg aagttctttg ctccatgagg aattctggta ccgcccggtt ttccccaggt
      301 ggcatcgtct gtgatcttta caactgcagg cacgggtccg tatcccacca ggaatttggt
      361 gacatcaccc aggatgggag ttttgaaagc acgctccagg ctggaatgaa agatggctga
      421 tactgttgcc tttgttttca tcttacatga tcaaatgagt atcatccccc gaaagcccct
      481 cgctgcataa taggattcag ctccgttgtg gtaggtgaat attcgcccga agcgatgatc
      541 acagaagatg gcaccgccca gcttgcgaac atcctcagga gtcttcaccc aactcgatgt
      601 tttggtgtcg aatggtccca gcttctgcag ccaggcatat tgttcctcat tgagcagttc
      661 tatccccatc tcagctgcca tgccgatggc actgttcttc ggtttgtgtt cctttctgga
      721 ttccagggca tcgggatcgt agcagaggct tcgtcttccc ttcggacttt cggcagcaca
      781 atccatgaag atgaaagcgc cggtatctgt ttcctgtcct accacatccg gttctccatc
      841 agtgatatcc atttcattca ggctgatcag tttgtccgga tttgcctcca gtctggcctg
      901 tacgtctttc cattgcatgc cggggtggcg gtgcatgtgt ttttcgaatc gtttttggag
      961 agttgacagc agcatgctgg ttgtttcttc tgaaagcatg tggtatcgtt ttatgattca
     1021 cgtatcaaca ggaaaacgtt cctgtcatcc attccggtca cttcatgggg aatgtcaacc
     1081 ggaatatcca tcgtatcgcc ggatcggagt gatatggatt cggtttcagt ctggaaatca
     1141 agtgtacctt ccaccacaca gatcatggcg ggattgctgg tcaggtgttt tttcagcagc
     1201 tgaccccgaa gcagacctat ggcagttacc tgtaccttat cagacctgtg gagcacctga
     1261 acagccggtt tttccgtgtt cagttcaaga gaatatatgt tgttgatcat tcaaattgca
     1321 tttattcaga tcaggatgtt cagtaaaagt aaggcttcag gaggaggtat ccggaaataa
     1381 tctggtcctg cgttttctca tttcattggc aatgtaagct gcatctcctc cctgttcctc
     1441 cagtctggtt atgatattgt catagctttc ttcatcccgg ttctggctga gcttaaagac
     1501 attttcgagt gtgtccactt ttatctcaaa gcccacaatg gccggcatca tgtgctccag
     1561 aaaagtatcg ggcagattat cataaatggt aggggaggag gggttgccct tttcaaaatg
     1621 cagggtgagt cttttcatca ggtcgatgag ttcatcatca tccatgaatc tgatctttcc
     1681 ctgtgcatgc acgctcatat agttccaggt actgccgatc tgcgggttgg tgtaccagga
     1741 ggcactaacg taagtactgg gtccggtaaa taccagcagg gcattcgggt tttccatgaa
     1801 ggccttgtga tgatctgtct tccgcatgat atgtccctgg atgaccaacc tgtcctcctg
     1861 tggaatgagc atcaccggta cctgggtggc tgcctgtcgc ccgtcagcaa aacttcctgt
     1921 caggaaggcg aatggatgtt cctgcatgaa ctgcagcacc actgcatgtt caggttcctt
     1981 gaagtaggaa agatcgtaca tgatacaaac ctaagaggtt tcgggattta aggtaggtga
     2041 agtatcagta gggtggttag tcattcccaa attgaagggc tatgcccgaa gacacggtat
     2101 agttggcatc cagatacata ttccccattg cccaaccagg tgttttagct gtcatagata
     2161 gctgaaagta cagcgctctt gtgatcggat agtttgcggt gagggtagcc aggcctccta
     2221 ttgcgctttt tgaagtggta aatgactgat cttcaggttg cgaccagatc atcaatcccg
     2281 gcgaaagaag aagcgtttta ttctttacta cgatctgttc atcaatgacc tcgatttcca
     2341 gtccgtggaa tgaatggttg tagttttgat acagatgata tgcaaagaag aagttcctga
     2401 ggggagtttg aagcatgata ttggtagaaa tatcagatcc aaatgacgtg agaatatgac
     2461 ggatagcaaa atttccataa tacgttccgc tggaatcaga acgtaaccgg atcctgtaaa
     2521 atgaatataa agccggcgaa agcaaattca gaaactgaag tcctccttgc tttttcagat
     2581 aatccagttg ttcctgggtt agatcggctg gtttaatata tcttttgata cctacgcctg
     2641 aagggtgaat tcctcgttct gcatacggtt cgtcaggatt gaacaggtca taggtccatg
     2701 cagccagatc cagtccggta aagtctctca cttcagtatt ggatccttcc tccagcatga
     2761 tctcatcagt cagctgattt gctgaactat ctgcacagga gttaacatag aaaatggagt
     2821 ttactgttga gatccagtac aatgagaggt tgggcagatc ttgatcatta taaaaattgg
     2881 tcttctgcat ttcctgaatc tgatcatatt gtgcttcgat gcctgcgctc atcaacctga
     2941 caaagtcggg gttggaatgg agtttcatgg atgcaagttc tgcatcatca atagaattaa
     3001 ctgaaactgt tctcttgccg atcgggaagg tattcatatc attgaaacta ttgatgtaga
     3061 acaatgtcat gacagaacgg tgatattctt catgcagcca gccgtttccg agcggggcat
     3121 atagcaacag cagatctgca cctgcagccg tcagagagct gaacagtatc ctcttaaagg
     3181 tatcttgctc atggaatagt cttttaattc cgtaatgagt tgtgagatac agatcattgg
     3241 aaagtgccag cgattgcgac atgctggggt tagcaaacat tttgaggtag tctgtaaaga
     3301 cagggtcatc aggagcacta acggtttggg aggcatatga aaggtaggga tattctatca
     3361 ggggaaactg caacctgatc ggtttctcat cattttgctg gaatgtctgt gcagccagat
     3421 acaatggaca gatgaacccg atgatgaacg gaagaacttt aaacagcttc ataaggtttg
     3481 gtttaatggg tggtcaatgg gcaggttttc tgtaattgct aagagactgg cagaaaaatg
     3541 ctccatgctg aacaacctgg atgatgctgc agcctctttg ctcatggtca tgtagtcact
     3601 taacggagcg ttcctgatgg caatgatcct ttccagatac tctttcgggc tatcgcaaaa
     3661 atagccgttt actccttcct gcaaaaagta cttgttctcg ccaacactgc tgcccagaac
     3721 gggaatgcca caggaaagac actgttttag tttgaaggca gatttgcctc gattgaattc
     3781 tgtatccagg agtggagaaa cgcccacgtc gaatctggca atttgatgat agacggacct
     3841 ctcatcaagc cagctgatgt ttagcggagc ctccacggaa atgtgatcat attttttgaa
     3901 gcttgatatg atatgttctg cctctccttc atttctggct cccaggacga tgaaccttac
     3961 cggaaaggga atttgtgtca atgccgggaa aaggagtgtt tccaggcttt cgagatgggc
     4021 accataatat cctatccacc cgattgtgaa aatgttctcc cgctcacgct tgatcacacc
     4081 atggtctatt acgggagagg tcagcaagaa cgcattcggg ttgagtggac tgatatactg
     4141 tatcagagat ctgctgcctg ctgagcaggc cgagcaatgc ttcataaagt ggtgtatcgt
     4201 atcagtggtt cggcgggtgt gttcggcatc atctgtatca tacagggtaa atgtaggcct
     4261 gaagaacaga agtaatttta atgcatgtgc atatattcct cctgtgaata ttttctgaaa
     4321 tacgatcaac gagttctttt ttctgaagat cagagcagat aagtatatcc ggataaaggc
     4381 tgcaatatgt ttcctgtcat aaccgggatg aactatggaa taggtgatat catgcgtttc
     4441 agcaagatat ctgagtggat agatgcagcg gtaacgaaca ctcggttcat cttcattgta
     4501 gtaagcaaac cagtatatgt gtttgaggtg cacaggtaaa tgctaatctt ttgatctttt
     4561 atggagttcc tgacggatct gttcgtttag ttcttgtttg ctgattccaa gcaacacttc
     4621 aattgtatga taatagtctt catcatgaac accaccagac atgagttcaa gtaacaacgg
     4681 ccaaccgcct ttttcaaata tcatttctgc gatcaatccg cctaagacat agtgaaaaga
     4741 tgtttcgttg tccagattgg ttagtttcag catttcattc aggtctgttt cggggtggct
     4801 ctgtaaatag cgatctgttc tttctatatg ccagtcaagg cttttaccac ggctgccgcc
     4861 taaaaatgtg gctatccctt cagcagccca gaagtgcatg tcaggaaagt agctgtcagt
     4921 ctctgcatgg aagacctcat gaatatagta ttcacctaag cctgcgcaat aaaccttgtt
     4981 gtctgaagct ctgccacttg gtaatgattg accgcccatg ccaaggtaat aatccagtcc
     5041 ttt
//
LOCUS       DAOMAX010000197         5022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2854_length_5022_cov_3.661768, whole genome shotgun sequence.
ACCESSION   DAOMAX010000197 DAOMAX010000000
VERSION     DAOMAX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2854_length_5022_cov_3.661768"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1447)
                     /locus_tag="PK742_09820"
     CDS             complement(29..1447)
                     /locus_tag="PK742_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU76927.1"
                     /translation="MHIEQIYTGCLAQGAYYITSNNEAVVIDPLREVQPYLDRANRDG
                     VTIKYILETHFHADFVSGHLDLAKKTGATIVYGPNANPSFDALIAKDGQELMVGDVTL
                     KVIHTPGHTMESTCYLLMDEEGKEHSIFTGDTLFIGDVGRPDLAQKAASMTQEELAAT
                     LFHSLRKKIMTLPENVVVYPAHGAGSACGKNMSNETMSTIGDQKVSNYALRADMTEAE
                     FVKEVTDGLLPPPAYFPMNVAMNRDGYTSFDEVRNQGMQELGVAAFEAAAEETGALIL
                     DTRDPAIYWKAFIPQSVNIGLNGDFAPWVGALVADVKQPILLVTEEGKVEETVTRLSR
                     VGFDNLIGHLEGGINAWIMEGKEVDSVDRITVEEFARRMEHSHSKVVDVRRETEYVGE
                     HVHDAWNRPLNYINQWINEVDPKEHFFIHCAGGYRSMIAASILQARGYRNFTEVEGGY
                     NAIRKTEIPRDNQVVENKLLKI"
     gene            complement(1563..2027)
                     /locus_tag="PK742_09825"
     CDS             complement(1563..2027)
                     /locus_tag="PK742_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HQU76928.1"
                     /translation="MNTPEQVLGYVKQFIESVGVDPSTCWNEQNKAYYIYKGSARIEI
                     FVSSHPENNGTTRHYLRIFSGLMKVPETNKERFYRRCLEVSDQSLGVKLTLVPNAAPD
                     NDWLYATYERDIQGIDYNETVTCINDMGLWADWLDDQLKSEFGEGGSPGSPN"
     gene            complement(2265..2780)
                     /locus_tag="PK742_09830"
     CDS             complement(2265..2780)
                     /locus_tag="PK742_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76929.1"
                     /translation="MRLLWACILLSAPAILSYGCSSKPKSDSIRITTVTIGGYKELND
                     FSNGLGPIVPGISITMVIKNQNNKPIFITNEDSLHLESTNGGAMMALLPKFNYTIDSA
                     GIEGILFTNDFSKSMISKLDLNDSLSFTNSFRALFPDYIRIHGIISGIMVDTLISTSE
                     VQTRNFIPIDN"
     gene            complement(2777..4747)
                     /gene="dnaG"
                     /locus_tag="PK742_09835"
     CDS             complement(2777..4747)
                     /gene="dnaG"
                     /locus_tag="PK742_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQU76930.1"
                     /translation="MLRSIHAVPYFYAVITKETIDRIMETARVEEVVGDFVSLKKRGS
                     NYIGLCPFHNEKTPSFNVSPSKGIYKCFGCGKAGNAVGFVMEHERFTYREALKFIAKR
                     YNIEVEETQRSEEQVQQDTVRESLYIINGFAQNYFHEFLLQADEGRNIGGSYFRERGL
                     NEATIQEFKLGFCPAGGSVFTDAAIAAGYQHELLKQLGLSNDQKRDFYRGRVIFPIHS
                     ISGKVLGFGGRTLSADKKVPKYVNSPESEIYNKSKVLYGMYQARQAVSSKDECFLVEG
                     YMDVLSLYQAGITHVVASSGTALTTDQVRLIKRYTKNLTILYDGDPAGVKAALRGLDI
                     SLEEGLNVKVMLLPQGEDPDTWVQKLGAEGFLEKVSKEKKDLIHLRTSLFLEEAAGDP
                     VKTAGVIKEIAFSISLIPDSIVRSLYIKSASTTLGLEEQILLQEVNRLIRKKAMDKLG
                     KEDRASAESVPDQTPTQPERKPHITFHFDEPQEKDIVRLLLENSNYELEGENAIVAIL
                     RNLEDVEIDNNLYKQVVDEYRKFYEQQEFLSQSHFINHEDETIRKLCLNLLQSPYELS
                     DNWEKMHEVYIKDKSYLVQKDIVKSISILKLKKVLRMKEEIENQLRQLQQDEAASQDM
                     QHAMEDIRQLQDFIRQLSEVTGTVVMPYTRNK"
BASE COUNT         1083 a         1225 c         1306 g         1408 t
ORIGIN      
        1 gcgcttttct attttcagta ttgctggatc agatcttcag cagcttgttc tccaccactt
       61 gattgtcgcg ggggatctct gtttttctga tggcgttgta accaccttcc acttcggtga
      121 agttgcggta gccgcgggcc tgcaggatgg acgctgcgat catgctccgg taaccaccgg
      181 cgcagtgaat gaagaagtgt tctttcggat ccacttcatt gatccactgg ttgatgtagt
      241 tcagcggtcg gttccaggca tcgtgtacgt gttctcccac gtattcggtt tcccggcgca
      301 catccaccac cttggagtgg ctgtgttcca tgcgacgggc aaattcttcc accgtgatgc
      361 ggtcaacgga atccacttcc ttaccttcca tgatccaggc gttgatacca ccttccaggt
      421 gtccgatcag gttatcaaat cctacacggc tcagacgggt cacggtctct tccaccttgc
      481 cttcttcggt caccagcagg atgggttgtt tcacatctgc taccagtgca cctacccagg
      541 gcgcaaagtc gccgttcagg ccgatgttca ccgactgcgg aatgaatgcc ttccagtaaa
      601 tggccgggtc gcgggtatcc agaatgagag caccggtctc ttctgcagct gcttcaaaag
      661 cagctactcc cagttcctgc atgccctggt tgcgcacctc atcaaagctg gtatagccgt
      721 cgcggttcat ggccacgttc atcgggaaat aggcaggagg gggcaggagt ccgtcggtca
      781 cttctttcac gaattctgct tcggtcatat cggcacgcag ggcgtagttg ctgaccttct
      841 gatcgccgat ggtggacatg gtctcattgc tcatgttctt gccacaggcg gaacctgcac
      901 cgtgagcggg atagaccacc acgttttccg gcagggtcat gatctttttg cgcagcgaat
      961 ggaagagggt agcggccagt tcttcctggg tcatgctggc ggctttctgt gccagatccg
     1021 gtcgacctac atctccgata aagagggtat cgccggtgaa gatggaatgc tctttccctt
     1081 cctcatccat taacaggtag caggtgcttt ccatggtgtg gcccggtgtg tgtatgacct
     1141 tcagagtcac atcgcctacc attagttcct gtccgtcttt agcgatcagg gcatcgaaag
     1201 agggattggc attggggcca tatacgatcg tagcaccggt cttttttgcc aggtccaggt
     1261 gaccgcttac aaagtcggca tgaaagtgcg tttccagaat gtatttgatg gtcacgccat
     1321 cgcggttggc acgatccaga taaggctgta cctcgcgaag tgggtcgatc accacagctt
     1381 cattgttgct ggtgatgtag taggcgcctt gtgccaggca tcctgtgtag atctgttcta
     1441 tatgcatgtc ttgtctttaa gcgttacctg aatgaaaccg cagagagggg catacggttc
     1501 cctgctctgt ggtaccacaa agatagaagg ccgggagggc agcttctgtg tcttttgtta
     1561 catcagttgg gtgaaccggg gctgccgcct tcgccgaact cgcttttcag ctggtcgtcc
     1621 agccagtcgg cccagagccc catatcgttg atgcaggtca cggtctcgtt gtagtcgata
     1681 ccctgaatat ccctttcata ggtggcatag agccagtcat tgtcgggagc agcattcggt
     1741 accagggtca gcttgactcc caggctctga tcgctcacct cgaggcagcg acggtagaaa
     1801 cgctctttgt tggtttccgg cactttcatc agtccgctga agatgcgcag gtagtgacgg
     1861 gtggtgccat tgttctccgg atgggaggat acgaagatct cgatacgtgc cgagcctttg
     1921 tagatgtaat acgctttgtt ctgctcattc cagcaggtgg agggatccac gccaacagat
     1981 tcgatgaact gtttgacgta gccaagtact tgttccgggg tgttcattta tggaagtttt
     2041 gctttaaggt aaagataaat tagacagttg gcaaaagagg aatagaatat tgttaaatgt
     2101 gagagggcag tcagcagtca gcagtcagca gtcagcagta aaaaaagcaa caagttattt
     2161 caatccgatc aatagataac tcatctgttc cgggtatagg gcatgaccac ggtgccggtc
     2221 acttcgctga gctggcggaa ggggaagttt ctggtttatg tctgttaatt atcaattgga
     2281 ataaagtttc ttgtttgaac ttcacttgtg ctgatcaatg tgtcaaccat tattcctgaa
     2341 ataatgccat gaattctgat atagtcaggg aagagtgccc tgaatgaatt tgtaaatgaa
     2401 aggctatcat tcagatcaag tttggagatc atcgatttgc taaaatcatt agtaaataaa
     2461 atcccttcaa ttcctgctga atctatggtg taattgaatt ttgggagtaa agccatcatc
     2521 gcacctccat tcgtggattc aagatggaga gagtcttcgt ttgtaatgaa aattggcttg
     2581 ttgttttgat ttttaataac catggtaatt gatattcctg gtactattgg accaaggcca
     2641 tttgaaaaat cgttcagttc tttatagcca ccaatagtaa cagttgtaat tctaatagaa
     2701 tcggatttgg gttttgagct gcatccatag gatagaatag caggtgcaga cagaagaata
     2761 caggcccaaa gcaatctcat ttgttccggg tatagggcat gaccaccgtg ccggtcactt
     2821 cgctgagctg gcggatgaag tcctgcagtt gtctgatgtc ttccatggcg tgctgcatat
     2881 cctgtgatgc agcttcgtcc tgttgcaatt gtcgcagttg gttctcgatc tcttctttca
     2941 tgcgaagcac cttcttcagc ttgagaatag agatggactt gacgatatct ttttgaacca
     3001 ggtatgattt gtccttgata tacacctcat gcatcttttc ccagttgtcg ctgagttcat
     3061 acggtgactg cagcaggttc agacacagct ttctgatggt ttcatcttcg tggttgatga
     3121 aatggctttg cgataagaac tcctgttgct cgtaaaactt ccggtattca tccaccacct
     3181 gtttgtacag gttgttgtcg atctctacat cttcgaggtt acgcagtatg gcaacgatgg
     3241 cattttcgcc ttccagttca tagttgctat tctcgaggag taatcggacg atgtctttct
     3301 cctgaggttc atcaaaatga aaggtgatat gcggtttgcg ttcgggttgt gtcggggtct
     3361 ggtcgggtac actttcggcc gatgcacggt cttccttccc cagcttgtcc atcgcctttt
     3421 ttcggatcag gcgattcacc tcctgcaaca ggatctgttc ttccagtccg agggtggtgc
     3481 tggcagattt gatgtacaga cttctgacta tactgtccgg aatcagcgaa atggaaaaag
     3541 cgatctcctt gatgacacct gcagtcttta ccggatcgcc ggctgcttct tccagaaaca
     3601 gggaagtacg cagatgtatc agatcctttt tctccttgct gaccttttcc agaaaacctt
     3661 cggcgcccag tttctgcacc caggtgtccg gatcctcgcc ctgcggtaac aacataacct
     3721 tgacgttcag cccttcttcc aggctgatgt cgagtccgcg aagcgctgct ttcacccctg
     3781 ccggatcacc gtcatacagg atggtcaggt tcttcgtgta gcgcttgatg agtcttacct
     3841 gatcggtggt aagagccgta ccactgctgg ccaccacgtg cgtgatgccc gcctggtaga
     3901 gggagagcac gtccatgtat ccttccacca gaaagcactc atctttcgat gatactgcct
     3961 gtctggcctg gtacatgcca tagagtacct tgctcttgtt gtagatctcg ctttcaggcg
     4021 aattgacgta tttgggtact ttcttatcgg cactcagggt gcgtccccca aagcccagga
     4081 cctttccgct gatgctgtgg atggggaaga tgacccttcc gcgatagaaa tcccgtttct
     4141 ggtcattgct cagtcccagt tgtttcagca gctcatgctg gtatcctgcg gcgatggctg
     4201 catcggtgaa aacggatccg cccgccggac agaaacccag tttgaattcc tggatggtgg
     4261 cttcgttcag tccccgctca cggaaataac tgccgccgat attcctgcct tcatcggcct
     4321 gcaacaggaa ttcgtggaag taattctggg caaaaccgtt gatgatatag aggctttcgc
     4381 gaacggtatc ctgctgcacc tgttcttcgc ttcgctgcgt ctcttctacc tcgatattgt
     4441 atcgcttggc aatgaacttc agcgcttccc ggtaggtgaa acgctcgtgt tccatcacaa
     4501 agcccaccgc atttcctgcc ttgccgcatc caaagcactt gtagattcct ttggacggag
     4561 agacgttgaa ggaaggggtt ttttcgttgt gaaaggggca aaggcctatg tagttggagc
     4621 cccgtttctt cagagagaca aaatcgccga ccacctcttc tacacgggcg gtctccatga
     4681 tgcggtctat ggtctctttg gtgatcactg cgtaaaaata tggaactgca tgaatagaac
     4741 gcagcatgcg ctgccgatgg tgcagaaatc acaacagaag gtctgccttg taggcatatt
     4801 tggcaaatat tcacaggccg ccggcgtttt gtgggtcagg aatagctcat ggaaggaagg
     4861 tgtggtctat atttgccggg catgaagtat ctggttttgg tttttacggt atcgatcgtc
     4921 ggactgagct cctgttcgcg tcaggtggcg aaagtggctg aaggtggatt gatatcagga
     4981 aggaccatcg ctgccttaca acccgataaa ttggcgggag ca
//
LOCUS       DAOMAX010000198         5018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2856_length_5018_cov_3.611928, whole genome shotgun sequence.
ACCESSION   DAOMAX010000198 DAOMAX010000000
VERSION     DAOMAX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_5018_cov_3.611928"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PK742_09840"
     CDS             complement(<1..806)
                     /locus_tag="PK742_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQU76931.1"
                     /translation="MTFDQLPLTAPFLRALEEEKYTHPTPIQEQAIPVILEGRDMIGC
                     AQTGTGKTAAFSIPMLQLLNTIAPGKGRRPIRGLVVTPTRELAMQIKESLDAYGRHCS
                     LRNTVVFGGVSQHGQVNALHRGVDILVATPGRLLDLFQQGYVKLDQLSFFVLDEADRM
                     LDMGFLPDVKRIIKLLPAKRQSLFFSATMPDNIRQLANTIVHDPAFISVTPAMSTAER
                     VEQSVYFIEHPDKKYLLLHLLREGNVRSALVFTKTKHGADKVSRMLSKAH"
     gene            complement(924..3461)
                     /locus_tag="PK742_09845"
     CDS             complement(924..3461)
                     /locus_tag="PK742_09845"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76932.1"
                     /translation="MNKWLITVLTLLVACSPVAEKHNETSEILEAMDHWSASRSYPFD
                     AIPEHAWMSAFEQARMIPSGRGSRSEIWTSLGPHNFAGRILCLAFNPQNPNTLYAGSA
                     SGGLWRSYTGGTGKNAWERVPSGFPVLSVSCISFEPGDSSTFYIGTGEVYNADGTGTG
                     MADRALRGSYGIGILKTTDGGATWTHSLDFGADAEKGVWNVAIAASAPEILYAGTTDG
                     VYRSGDGGENWSLIFDEDMVFDILVHPDDPDKLVLGAGNFNSSGRGIWWSDDGGSNWT
                     HVSSGLPPDFLGKIQLDASQSSPEIVYATIGNGFNTDVETSSYLCNSEDFGHTWEVIN
                     STNFAVYQGWYSHDVSIHPADPDHLAVIGLAVYTSTNGGNSLYAKTSTATPGYSYPEI
                     EGPNGPGNYVHADCHAVKWHPTEPDVFYVATDGGIYRTDDAGESYYSCNGRLQTVQFY
                     NGFSVSGQDSLLCYGGLQDNGSIKLIDDLDTETGLYLKWQHVFGGDGGWTAINPAEDY
                     IHYVSWQGLNVLVSDGPGYSSLSIPKIWPVAFIAPYVICPSDETRMYAGSAGVASSTN
                     GGADWSLTNGGTALDGNPVISMDISFNDPDVLYVSTAPYGGNPGHVFVTSNGGDTWSN
                     ISSGLPDRYVLDLHVDPNNDAIAYAAIGGFGSGHLYRTTNYGSTWFDISYGLPDVPTN
                     AVVVDPDLPATIYVGNDLGVYVSEDNGSTWMAYTDGMPEAAMVFDLKIQQDSRKLYAA
                     THGNGAFWSDLIKTDDPPAMTNQYQLLPLQVFPNPAYDQITVTPPKAMSGSILLTLTD
                     LHGRILHRETAKSSASIQLYRLNLPAGTYLLELSDGRSTVSTKVFWL"
     gene            complement(3528..4079)
                     /locus_tag="PK742_09850"
     CDS             complement(3528..4079)
                     /locus_tag="PK742_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76933.1"
                     /translation="MKILLAAGIALASLIQPSRPVFPGCEAITKLTNQYKLQDGSLKG
                     EQSFTLNTLFYDTIAYFECPVLIEGAVHAFVTGPENGSEVYWVEQVMGAGLQKEEALQ
                     LFSSLSESYRKCYPDATSEMDLTPESQDDLDIWAKWAFTEADGTYCDLWLYADFDAMF
                     EDMLQDPESKATFFQVVLIIGRE"
     gene            4182..>5018
                     /locus_tag="PK742_09855"
     CDS             4182..>5018
                     /locus_tag="PK742_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HQU76934.1"
                     /translation="MIRLHITLLFVFLLAGIQMSQAQETVVLAVGQYGDLVFTEDTTT
                     GDVVIPLRRVQNLFVLEGIVDGQRGNFILDTGAPGLVLNAAYFNKGKVLESASSFGVT
                     GGGDQVYRYAADSLIVSGLSFYDLQANVADLGHIENARGIRILGLIGSGMFSRLEMVL
                     DTRASTLGLYRVDRSGNRVFTPDELPAADLEIPLEYSSSLMFIRGVAAGKKLRFVLDT
                     GAENNVLSTSSPSRVIKEIRVTNLTTLKGAGASTLEVASGRLPDLQLGGYDFDSMTFL
                     LTD"
BASE COUNT         1241 a         1230 c         1291 g         1256 t
ORIGIN      
        1 atatgggctt tgctgagcat cctggatact ttgtcagcac catgcttggt cttggtaaat
       61 accagcgcag acctgacgtt tccttcgcgg agcaaatgca gaagcaggta tttcttatcc
      121 ggatgttcga tgaaatatac gctttgctca acccgttcag cagtactcat ggcaggcgtc
      181 acactgataa aggcaggatc gtgcacaatg gtattggcaa gctgtctgat attatccggc
      241 atggttgcag aaaagaacaa tgattgtctc tttgcgggca gtagtttgat gatccgcttc
      301 acgtctggca ggaagcccat gtccagcatg cggtcggctt catccagtac gaaaaaggaa
      361 agctgatcca gcttgacata tccttgctgg aacagatcga gcagacgacc gggtgtagct
      421 accagaatat ctaccccgcg gtggagggca ttcacctgtc cgtgctggga tactccgcca
      481 aatacaacgg tatttctaag ggaacagtgg cgtccgtagg cgtccaggct ttccttgatc
      541 tgcatagcca gttctcgggt aggtgttacc accagcccgc gaatggggcg gcgacctttt
      601 ccgggtgcaa tcgtatttaa aagctgcagc atggggatac tgaatgctgc ggtcttgcct
      661 gtgccggttt gcgcacagcc tatcatgtcg cgaccttcca gaataacggg tatggcctgt
      721 tcctgaatag gagtggggtg ggtatatttt tcttcttcca gcgcacgcaa aaagggcgcc
      781 gtaagaggca attgatcgaa tgtcatgttt atttgtgttt aaagcttgcc ggaaggacta
      841 caggaagcac tattcggaaa gacggtgcaa aggtaagcct attttacctt aacttctcag
      901 ttttccaaaa taccgggcat taatcacagc cagaacactt tagtgcttac agttgaccgt
      961 ccatctgaca attcaagcag gtaggtgcca gcaggtaggt tcagcctgta caattgtatg
     1021 gaagctgagc tcttggccgt ttcccggtgt aggatccttc catgaagatc tgtcagggtc
     1081 agaagaatgg agccagacat ggccttcggg ggcgttaccg tgatctgatc gtatgccgga
     1141 ttcgggaaaa cctgtaaagg cagtaactgg tattggtttg tcatagccgg cgggtcatcc
     1201 gttttaatca ggtcgctcca gaaagcccca ttgccgtgtg tagcggcata cagtttcctg
     1261 ctgtcctgct ggatcttcag gtcaaagacc atagcagctt cgggcattcc atctgtatag
     1321 gccatccagg tgctgccgtt gtcttctgac acatagacac cgaggtcatt tcccacgtag
     1381 atcgtggcag gcaggtcagg gtcaaccaca actgcattgg tgggcacatc aggcaatccg
     1441 taagagatat cgaaccaggt ggatccgtag ttggtagtgc ggtacaaatg tccgctgccg
     1501 aaaccaccga tagcagcata tgcaatggca tcattgttcg ggtccacgtg caggtccagc
     1561 acatacctgt ctggcagtcc gctgctgata ttgctccagg tatcaccacc attggaggtt
     1621 acaaaaacat gccccgggtt tcctccgtaa ggtgcggtgg aaacatacaa cacatccgga
     1681 tcattgaatg agatatccat agatataact gggttaccat ccagtgctgt tcctccgttg
     1741 gtaagtgacc agtcggcgcc accattggtg cttgaagcta ctccggcaga accggcatac
     1801 atccgggttt catcagaagg gcagatcacg tagggtgcga tgaatgccac cggccagatc
     1861 ttcggaatgc tcagactgct gtaaccgggt ccgtcagaga ccagcacatt caatccctgc
     1921 cagctaacgt agtgaatata atcttcggca ggattgatgg ctgtccatcc gccatctcct
     1981 ccaaacacat gctgccattt cagatataat cctgtttcag tatccaggtc atcgatcagt
     2041 ttaatggaac cattatcctg cagtccgcca tagcacaaca gactatcctg ccctgaaacg
     2101 gaaaatccgt tatagaactg aacagtttgc aaccggccgt tgcaggaata atagctttca
     2161 ccggcatcat cagtccggta gatgccacca tcagttgcca catagaaaac atcaggttct
     2221 gttggatgcc atttgacggc atgacaatct gcatgcacat agtttccggg accgttgggt
     2281 ccttctattt ccggataact gtaaccaggt gtagcggtgg aagtttttgc atacaaagag
     2341 ttgcccccgt tcgtgctggt ataaacggcc agaccaatta cagccaggtg atcggggtct
     2401 gccggatgaa tgctgacatc atgagaatac catccctgat agactgcaaa attggttgag
     2461 ttgatgacct cccaggtatg gccgaagtct tcgctattac acaggtagct gctggtctct
     2521 acatcagtat tgaaaccatt gccgatggtg gcatatacta tttcaggcga tgactgggaa
     2581 gcatctaatt gtatcttccc caggaaatcc ggaggaagtc ctgatgatac atgggtccag
     2641 ttcgatccac catcatcaga ccaccagata cctctgccag atgagttgaa atttccggct
     2701 ccaagtacca gtttgtcagg gtcatcgggg tgtacaagaa tatcgaacac catatcttca
     2761 tcaaagatca gcgaccagtt ttcgccacca tctcccgatc tgtaaacgcc atcggttgta
     2821 ccggcataga ggatctctgg tgcagaagca gctatcgcca cattccagac acccttttca
     2881 gcatcagcac caaagtccag agaatgggtc caggttgcgc caccatcggt ggtcttcaga
     2941 atgccgatgc catagcttcc ccggagggca cggtctgcca tacctgttcc ggttccgtca
     3001 gcattgtaaa cttctccggt accgatataa aaagtggatg aatcgccggg ttcaaaactg
     3061 atacaactta cactcagcac gggaaatccc gaaggcacac gctcccatgc atttttaccg
     3121 gttccgccgg tataggaacg ccataacccg ccacttgcag aacctgcgta cagggtattc
     3181 gggttttgcg gattgaaggc caggcaaagg atcctgcctg caaagttgtg aggccccaga
     3241 gaggtccata tttcacttcg gctgcctctt ccggaaggga tcatccgggc ttgttcaaat
     3301 gccgacatcc aggcgtgttc cggaatggca tcaaaaggat aggagcggga agcagaccaa
     3361 tgatccattg cttccagaat ctcagaagtt tcattgtgtt tctcagctac cggagagcag
     3421 gccaccagca atgtcagaac agtgatcagc catttgttca tatcatgaat atacgaacag
     3481 cggttggaaa gcaaccctgc aggcagcgaa tatgatagtt aactgaccta ttcccggcca
     3541 atgatcagca ctacctgaaa gaatgtggcc tttgattccg gatcttgcag catgtcttca
     3601 aacatggcat caaaatctgc atataaccac aggtcgcaat aggtgccatc tgcttctgtg
     3661 aacgcccatt tggcccagat gtccagatca tcctgtgatt ctggcgtaag atccatttca
     3721 gaggtggcgt caggatagca cttgcggtat gattcagaca ggctgctgaa cagttgaagg
     3781 gcttcttctt tctgcagtcc ggctcccatg acctgttcta cccagtacac ttcagaacca
     3841 ttttccggtc cggtaacaaa agcgtgtaca gctccttcta tcagcaccgg gcattcaaaa
     3901 tatgctatgg tgtcatagaa aagagtattg agggtaaagg attgttcacc tttcagagac
     3961 ccgtcctgga gtttatactg gttggtcaac ttagtgatcg cttcacaacc ggggaaaacc
     4021 ggccgggatg gctggatcag agatgccagt gcgataccgg cggctaatag gatcttcatg
     4081 tgtgttgttt ccgttttgtt ggtgaatata cataagatgc tgccggcatg cagatttcac
     4141 aaggatctgg tattgcgtat atttgaatac ggatgtcagg catgatcagg ctacatatca
     4201 cactactctt tgtcttcctg cttgcaggca ttcaaatgag tcaggctcag gagaccgtag
     4261 tactggcagt tgggcaatac ggcgacctgg tctttacaga agacaccacc accggcgatg
     4321 tggtcatacc cctcagaagg gtgcagaacc tgtttgtgct ggagggaata gttgatggtc
     4381 agcggggaaa tttcattctg gatacaggag ctccgggcct ggtacttaat gccgcatatt
     4441 ttaacaaagg gaaggtgctg gaatctgcca gctcatttgg tgtgaccggt ggcggcgatc
     4501 aggtgtaccg gtacgctgca gattcactca ttgtttcagg gctgtcattc tatgacctgc
     4561 aggctaatgt ggccgatctt ggtcacattg agaacgccag gggaatcagg atacttggac
     4621 tgataggatc aggaatgttc agcaggcttg agatggtgct ggatacccgt gcttctacac
     4681 tggggctgta tcgtgttgat cgaagcggaa acagggtttt tacccctgat gaattaccgg
     4741 cagcagatct tgagatcccg ctggagtata gtagcagcct catgttcata agaggtgttg
     4801 cagccggtaa gaaacttcgt tttgtactgg acaccggggc agagaataat gtactcagta
     4861 catcctctcc gtcacgggtc atcaaagaga tcagagttac caacctgacc accttgaaag
     4921 gagcaggagc gagcaccctg gaggtagcca gcggacggtt gcctgatctg cagctgggtg
     4981 gatatgattt tgactccatg acctttttac tgaccgat
//
LOCUS       DAOMAX010000199         5016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2860_length_5016_cov_4.612377, whole genome shotgun sequence.
ACCESSION   DAOMAX010000199 DAOMAX010000000
VERSION     DAOMAX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2860_length_5016_cov_4.612377"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PK742_09860"
     CDS             complement(<1..996)
                     /locus_tag="PK742_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76935.1"
                     /translation="MKMLTWICAVGMSLSLQAQNIFGTWQNTAYQMQYTFTQEGTYQF
                     SSTQFGQASGNYLLQGGYLYLYDANNNPSVQYYLSGITAQQLHLTDVNQVQFTLDRVG
                     VAPEVKGMEAFSKSKYPRVLAGSGKQQIIEADARLYAAAISFLVQTNIPEIDYKKIES
                     ALIKDFKTDAASTMTDLQALRSGMEYIFTLHDPVEIGLVRQQILGNIYWMSVVNKHAS
                     VYWDVTDSYTDVIAFDETNKLVLTQKDLDDYLDYLSLAYQNYGQQLTAAMRSELAQQM
                     VSNFAALRLEDKQLLACGSLLKDNLVAQMNAMSTREQQQFQQHLQQQPPSVDWSGA"
     gene            complement(1075..4008)
                     /locus_tag="PK742_09865"
     CDS             complement(1075..4008)
                     /locus_tag="PK742_09865"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HQU76936.1"
                     /translation="MKKILTLCSLSLPLAVMAQFTSFVNPFIGTGGHGHTYPGASMPF
                     GMVQLSPDTRLEGWDGCSGYHYSDNVIYGFSHTHLSGTGIPDYCDVLLMPTVGEMQFN
                     NMTGNDPKTGYASAFSHDKEKASPGYYSVLLEDDNIPVELTVTNRVGMHKYSFPASDE
                     ANVILDLRHRDKLLDGTYIRVVSDTKVEGLRRSSSWAEDQWLYFALEFSQPFSESVID
                     DDKEKRVIANTGAVYGTSLYAGFRFKSNGKEPLLVKVSVSAVSAEGAWKNMQAEVSGW
                     DFNAVRKAADDAWNKELSKIEISTKDKDEKTIFYTALYHCMLAPNLYMDADRRYRGMD
                     GQIHTAGGFDYYTVFSLWDTFRALHPLLTIIDQKRTNDFINTFIKQYEQSGYLPVWEL
                     AGNETNCMIGVHSISVIADAAVKGILGYDTEKAYMAMQQATKTNEYGWRQYRDIGMIE
                     SNHAAESVSRTLEYAYDDWCISQMGQVLSHDDYFTFYRRSMNWINLFDGTFIRPRTNG
                     YWYEPFDPFEVNFNYTEANAWQYNFFAPHDLGLLLPQNDQRSLDASRKRLDDLFTAPS
                     VTSGREQADITGLIGQYAHGNEPSHNFAYLFNFVQQPEKTQYYVDRIKREFYTNAPDG
                     LVGNEDCGQMSAWYVLSSLGFYPVCPGDPVYSIGSPSFPEATIHLENGKSFHITADKA
                     GGDNIYVQSATINGQQQMLPNISHRAIIEGSTLGMVMSNNASTGFSGDTSTARKRLMP
                     QPPLIHVDSPVFTDSVVVTITQFDTTDNLVYQTHGDVPLIKPINYTGPLVFHNDVMVK
                     AISKTRTGDAGYVSTAILKKIPENFHITYQTAYDNQYTAGGDLALIDGQRGGKDFRMG
                     KWQGWQGRDMQVLIDLGEERSISEVGAGFLQDIRAWIWLPTTLEVFVSTDAVQFSQGA
                     MTRHTVTTTDYDSQTTENLKVSFETPLTGRYVMITARNAGTIPSWHPGAGGQSWIFCD
                     EIWVK"
     gene            complement(4094..>5016)
                     /locus_tag="PK742_09870"
     CDS             complement(4094..>5016)
                     /locus_tag="PK742_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQU76937.1"
                     /translation="TGIKFGLVTRNGEILHTDKVKTKGYPKPDPLPEDLKKRIAEPLK
                     KLGAKVAGVGIGTPNGNYFDGTIDQAPNLPWKGLVPMKALFKKTFRKPVALTNDAKAA
                     ALGEMYFGAAQGCRNYLFITLGTGLGSGQVVNGKLIYGHDSLAGELGHVIVYRNGRLC
                     GCGRKGCIEQYASATGIVKTYYELLRNKNVDSFRLSLGNMIDAEKICALALEEDEIAL
                     EAFSRTGWILGFHLANSVCYTRPEKIFLFGGLAQAGDLLFEPTIRSFEDNLMNIYQDK
                     IEILPSGLKESEAAILGAASLIWGEMEDDS"
BASE COUNT         1171 a         1233 c         1241 g         1371 t
ORIGIN      
        1 ggcacctgac cagtcaactg acggaggttg ttgctgcagg tgttgctgaa actgctgctg
       61 ttcccgggta ctcatggcat tcatctgcgc caccagattg tctttcagca gcgaaccaca
      121 ggccagaagt tgtttgtctt ccagccttaa ggcagcaaaa ttgctgacca tttgttgtgc
      181 cagttcactg cgcatggcag cagttagttg ctggccgtaa ttctggtagg ccagtgacag
      241 gtagtccagg taatcatcca gatctttctg tgtcagtacc agtttattgg tctcatcaaa
      301 tgcgatcaca tcggtgtagc tatcggtcac atcccaatag acactggcgt gtttgttgac
      361 cacactcatc caatagatat tacccaggat ctgttgcctg accaatccga tctcgaccgg
      421 atcatgcagg gtgaagatat attccatacc gcttcgcagt gcctgcagat cggtcatggt
      481 agatgctgca tctgtcttga aatcttttat cagagcagat tctatttttt tgtaatcaat
      541 ttcaggtatg ttggtttgta ccagaaatga aatagctgcg gcatacagac gtgcatctgc
      601 ttcaatgatc tgttgtttgc cgctgccagc aagtaccctg ggatatttgc ttttgctgaa
      661 cgcttccatt ccctttactt caggagcaac tccaaccctg tcaagcgtaa actgcacctg
      721 attcacatca gtgagatgga gttgttgcgc tgtaatgcca gacaggtaat actgcacact
      781 tggattattg ttggcgtcat agagatacag gtagccgccc tgtaacaggt agttgcctga
      841 tgcctgacca aattgagtac tgctgaactg ataggttcct tcctgggtaa aggtgtattg
      901 catctggtaa gcagtgtttt gccaggtgcc aaaaatgttt tgcgcttgca gtgacaggga
      961 cattccaact gcacaaatcc atgtaagcat tttcatgccg ctaaaataat aaaagcgaaa
     1021 agtgtggaca gatatcagcc acacttttcg cttaattcag ttcagcaatt gcagttactt
     1081 cacccagatc tcatcacaga agatccagct ttgaccaccg gcacccggat gccaggatgg
     1141 tatggttcct gcgtttcttg ctgtgatcat cacataccgt ccggtaagtg gtgtctcaaa
     1201 agatactttg aggttttcgg tggtttgcga atcatagtcg gtggtagtga ccgtgtgtct
     1261 ggtcatggcg ccctggctga attgtacagc gtcggtactt acaaatactt cgagtgtggt
     1321 gggcagccag atccaggcac ggatatcctg gaggaaaccg gcacctactt cactgatact
     1381 tcgctcttcg cccaggtcta tcagcacctg catatcgcgg ccctgccatc cctgccattt
     1441 gcccatccgg aaatcctttc cgccccgctg accgtctatc agtgccagat caccaccggc
     1501 agtatattgg ttatcgtaag ccgtctggta agtgatatgg aaattttccg ggatcttttt
     1561 gaggatggcg gtcgatacat atccggcatc gccggtacgg gtcttgctga tcgcttttac
     1621 catcacgtca ttatggaata cgagcggacc ggtatagttg atcggtttga tgaggggtac
     1681 atcaccgtgg gtctgatata ccagattatc tgttgtatca aactgagtta tggtaaccac
     1741 cacagagtct gtaaatacgg gagagtccac atgaatgagc ggaggttgtg gcatcagtct
     1801 tttgcgggcg gtgcttgtgt cgccgctgaa gccggtgctg gcattattgc tcatgaccat
     1861 gccaagggtg ctgccttcta tgattgcccg gtgacttata ttcggcagca tctgctgttg
     1921 accgttgata gtggcagatt gtacatatat gttatctccc cctgccttat cggcagtaat
     1981 atgaaatgac tttccgtttt cgaggtggat ggtagcttcc ggaaagctgg ggctgcctat
     2041 gctgtacaca ggatctccgg gacaaactgg atagaatccg agtgagctca ggacatacca
     2101 tgcactcatt tgtccgcagt cttcattgcc taccagtccg tctggtgcgt tggtatagaa
     2161 ttcacgcttg atgcggtcca cgtaatactg ggtcttttcc ggttgttgca cgaagttgaa
     2221 gagataggca aagttgtggc tgggttcatt gccatgtgca tattgcccga tcagcccggt
     2281 aatgtctgcc tgttctctgc cactggttac cgatggagcg gtgaagagat catccagccg
     2341 cttgcgtgat gcatccaggc ttcgctgatc attttggggc agcaacaagc ccagatcgtg
     2401 tggtgcaaag aagttatact gccaggcatt ggcttctgta tagttgaagt tcacttcgaa
     2461 cggatcgaat ggttcatacc agtagccatt ggtgcggggt ctgatgaaag tgccatcgaa
     2521 caggttgatc cagttcatgg accgacgata gaaagtgaag tagtcatcat gactcagtac
     2581 ctggcccatc tggctgatgc accagtcgtc ataagcatat tccagtgtac ggctaacgga
     2641 ttcagctgca tggttgcttt caatcatacc aatatctctg tactgtctcc agccatattc
     2701 attggtcttc gtagcttgtt gcatggccat atatgctttc tccgtatcat agcccagaat
     2761 accctttacg gcagcgtctg ctattacact gatggaatgc accccgatca tacagttggt
     2821 ctcattgccg gccagttccc agacaggcag gtatccgctt tgttcgtatt gtttgatgaa
     2881 agtgttgatg aaatcgttgg ttcgtttctg atcaatgatg gtcagcagcg gatggagggc
     2941 acggaaagta tcccagaggg aaaacacggt atagtaatcg aaaccaccgg ctgtatggat
     3001 ctgaccatcc atgccgcggt atcttctgtc ggcatccata tacaggttag gtgccagcat
     3061 acaatgatag agtgcagtgt agaagatcgt tttctcgtct ttgtctttgg tgcttatttc
     3121 tattttagac agctctttat tccaggcatc atctgcagct tttctcactg cgttaaaatc
     3181 ccatccgctc acttcggcct gcatgttttt ccaggcacct tcggcagata cggcagacac
     3241 agataccttc accagcaagg gttcttttcc attgctttta aaccgaaatc cggcatataa
     3301 tgaagtgcca tagactgcac cggtattggc gatgaccctt ttctccttat catcatcaat
     3361 cacagactct gagaagggtt gagagaactc taaggcgaaa tacaaccact gatcttctgc
     3421 ccaggaggag gatctgcgca gcccttccac ttttgtatcg ctgaccaccc ggatatacgt
     3481 accatccagc agtttatcac gatggcgaag gtccaggatc acgttggctt catcagaagc
     3541 aggaaaactg tacttgtgca tgcccacccg gttggtcacg gtcagttcta ccggaatatt
     3601 atcatcttcc agcagcacac tgtagtaacc gggcgaagct ttttccttgt catgactgaa
     3661 ggcagaagcg taaccggtct ttgggtcatt gcctgtcatg ttgttgaact gcatctcacc
     3721 cacagtgggc atgagcagta catcacaata atcaggtatg ccggtaccac tgaggtgggt
     3781 gtggctgaag ccatagatta cgttatcgct gtagtggtaa ccgctgcaac catcccaacc
     3841 ttccagccgg gtatcaggac ttagctgcac catgccaaag ggcatggaag cgccgggata
     3901 ggtgtggcca tgtccgccgg tgccaataaa tggattgaca aaagaagtga attgagccat
     3961 cactgccagg ggcagggaaa ggctgcataa tgtgaggatc tttttcatat gaatagatag
     4021 cgccatttgg caggagaatg tagcaaaata agggccagaa gccggttaat aaaaagactt
     4081 tcgtcaatgg cactcaggag tcatcttcca tctctcccca gataagggaa gcagcaccca
     4141 gaatagcggc ttcactttcc ttcaggccgc ttggcaggat ctcgatctta tcctgataaa
     4201 tattcatcag gttgtcttca aaactcctga tagtaggctc aaacaacaga tctccggcct
     4261 gagcaaggcc accgaacaaa aagatctttt ccggtcgggt atagcaaacc gagttggcca
     4321 gatgaaaacc caggatccag cctgtgcggc tgaaagcctc cagggcaatt tcatcttctt
     4381 cgagagccag ggcgcagatc ttttctgcat cgatcatatt gcccagactc agtctgaagc
     4441 tgtcaacatt cttgttgcgc agcaactcat aataggtttt gacaataccg gtagcactgg
     4501 catattgttc tatacaacct ttgcggccac agccgcacag gcgaccattc cggtacacga
     4561 tcacatgacc gagttcgccg gcaaggctgt cgtgcccgta tatcagcttg ccattgacca
     4621 cctgcccgct gcccaaccct gttcccagag taatgaacaa atagttcctg catccctgcg
     4681 cagcaccgaa atacatctca cccagtgcgg cagctttggc gtcgttggtc agggcaaccg
     4741 gtttgcggaa tgtttttttg aacagcgcct tcatgggcac caggcctttc caggggaggt
     4801 tgggagcctg gtctatggtt ccgtcaaagt aattgccgtt gggggtgcca attcctacac
     4861 cagctacttt ggcacccagt tttttcagtg gttctgcaat gcgttttttc aggtcttcag
     4921 gcaagggatc gggtttgggg tagcctttcg ttttgacctt atcggtatgc aggatctctc
     4981 cattgcgggt taccagcccg aatttgatcc cggttc
//
LOCUS       DAOMAX010000200         5016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2862_length_5016_cov_3.632735, whole genome shotgun sequence.
ACCESSION   DAOMAX010000200 DAOMAX010000000
VERSION     DAOMAX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_5016_cov_3.632735"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..772)
                     /locus_tag="PK742_09875"
     CDS             complement(224..772)
                     /locus_tag="PK742_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HQU76938.1"
                     /translation="MFTSIILSGLLFLQPVTGGEETSASEGIQWLTLEEAVALSAKEE
                     KPLLIDFYTDWCGWCRKLDATTYQDQKVIDYVNKYFYAVKFDAESQDSYEFNGKTYGF
                     KAGGGRGTNEFALKYASRNGRLGYPTTTFMFADGESLMVVPGYQDANQMTMLLRYLGE
                     EHYKTQDFNAFKASVIAAESAD"
     gene            complement(782..1387)
                     /locus_tag="PK742_09880"
     CDS             complement(782..1387)
                     /locus_tag="PK742_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQU76939.1"
                     /translation="MSKKYYLLVALIVVAGIVVASFTVQKSSVVPEKTPVTLTGEETT
                     EIKWYSVNEALALHEKTGKKLFIDVYTNWCGPCKWLDQNTFHHPVIMDQLEKYYIPVK
                     FNAEGNDTIVFNGNTFVNPNPEAGPRRSIHQFTQYIASTERGISYPTMVFLNEQLEMI
                     QPITGALGPDQMEPILEFIGADHYLSTPWEDYLQTFQSNIE"
     gene            1476..2039
                     /locus_tag="PK742_09885"
     CDS             1476..2039
                     /locus_tag="PK742_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HQU76940.1"
                     /translation="MGRRIVIGITGASGAIYAQVLLDAIAAMKEPPEAVGVVMSSNAK
                     TVWQHELGQEMPDYPAFHFYASKDFMAPFASGSAQYDTMIICPCSMGTMGRIAAGISN
                     DLMTRAADVVLKERRKLILVARETPYSLIHLRNMETITLAGGIICPATPSFYSRPSDM
                     RALAATVTDRVLDLAGFSNASYRWGNS"
     gene            2121..4145
                     /locus_tag="PK742_09890"
     CDS             2121..4145
                     /locus_tag="PK742_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HQU76941.1"
                     /translation="MKFAAVLLLCSFSCSLQAQTIPAERTVNWSLAGLEADCFADWPV
                     LNIISAGGYADGSTSNDAVLAEVLDGLTEPTIIYFPSGDYLFEDQIALPSGVVLRGNG
                     ADHTTLIFDLAAEKDLIAAKGSAGAVTVSIIGAPAKGNSVVKVSDPSSFSPGNFIIVN
                     DDDTELVNNTWALGTTGQIVRIISIAGNNLTLNEPLRRSYPADRSPYVRSMNMVTDVG
                     IEALGISNLSPMSSQRSNIFFRYVWNGYVSCVESYKTNYAHVELEHAAHCAVTGSYFQ
                     DAWAYDDGGKGYGVLAHYAAGANLISGNIFKHLRHSMLVQAGANGNVFSYNYSEDPFW
                     TGTVLPSDAAGDLVLHGNYPYANLFEGNILQNLVVDDSHGLNGPYNTFFRNRADNYGI
                     VMSNASVGNDMHFIGNEVTNTGFLKGLYSIYGSGHIEFGNNIKGTVTPAGTSPLPEQT
                     LYLGSVPSFYLTTGNWPPIGIPNAISSEDIESHYRFVSGDLTLCADCGPSCSYPEDLN
                     AGPVLINKASLSWTTVTDASRYEVQYKPSGAGPWNKVKPLENNVLLTGLMPGTNYVWK
                     VRSRCPDGWTAFSPNTTFTTLSMRAGEGPKADIFPNPATDALSIHLHADGGVIQIMLT
                     DLHGRRAYTETVTHEGGQWIHSIRPLPAPGIYLLQVYLKDEVITEKVVIE"
     gene            complement(4148..>5016)
                     /locus_tag="PK742_09895"
     CDS             complement(4148..>5016)
                     /locus_tag="PK742_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU76942.1"
                     /translation="AEFYGYFYGHPSSHTVVIQEDSGTTRTVILEGVPKPSMISKRQE
                     LYPQNDLDPEAGKGITLQFSNDSSVAILTIRDFHNKVLRRDYGQYFKGEIREIFEEIE
                     ASGTKRLVLDLRNNQGGEIRNGYLLLRYIMNEPFYMMYSYNMVSSKFPKRSQMRLKDV
                     AGPYTGLADPADRHAFTGQLAVLVNGGSFSNSAIVTSVIDRYQRGIVVGEETGGNGYI
                     ISGDSRMLHLPNTGIVAYIPRRQYVIQDPAGNNRRGIIPEFQISPELQDLIKGNDPAM
                     QKAIELLSAAVQ"
BASE COUNT         1256 a         1260 c         1211 g         1289 t
ORIGIN      
        1 gagcctccct tttcaatctg tgagggagac tccgggtcaa gcccggagaa acattcaatt
       61 taaaagtgaa atccttcgaa agcagaccac aatcaaccct ctgcgtcctt tgtgtattct
      121 ttgtgccttt gtgttcaaac cctgagatct ttatggaata aaatttcacc tcagctactg
      181 cttctccatc ctccgagccg gagaaacata aactctgatg tgatcaatct gcagactcag
      241 cagcaatcac gcttgccttg aatgcgttga agtcctgggt cttgtagtgt tcttcgccca
      301 ggtagcgcag cagcatggtc atctggtttg catcctgata tccgggtacg accatgagtg
      361 attcaccgtc ggcaaacata aaagttgtag taggataacc caggcgcccg tttcggctgg
      421 catacttcag ggcgaactca ttggtacccc ggccaccgcc ggctttgaat ccgtaggtct
      481 tgccattgaa ctcataggag tcctgcgatt ctgcatcgaa cttcaccgca tagaaatatt
      541 tgttcacata atcgatcact ttctgatcct ggtaggtggt agcatccagc ttacggcacc
      601 atccacacca gtcggtatag aaatcaataa gcaatggctt ctcttctttg gcgctcaggg
      661 ctactgcttc ttccagcgtc agccactgta tgccttcaga tgcagaggtt tcctctcctc
      721 ctgttaccgg ctgaagaaac agcaggcctg ataaaataat gcttgtgaac atggcggtaa
      781 tttattctat gttggactga aaggtctgca gataatcttc ccagggcgta ctgaggtaat
      841 gatctgcacc aatgaattcc agtatgggtt ccatctgatc agggccaagt gctccggtaa
      901 tgggttgtat catttcaagc tgttcattca gaaaaaccat ggttggatag gaaatgcccc
      961 tttctgtgga tgctatatac tgggtgaact ggtggatgct tcttcttggt ccggcttccg
     1021 gattcgggtt gacgaaagtg ttgccgttga aaacgatggt atcgtttcct tcagcattga
     1081 atttgaccgg tatgtagtac ttctccagct gatccatgat gacgggatgg tgaaaggtgt
     1141 tctgatccag ccatttacaa gggccacacc agttggtata gacatcaatg aacagctttt
     1201 taccggtctt ttcatgcagt gccagagcct cgttgacaga gtaccatttg atctcagtgg
     1261 tttcctcacc tgtcagggta accggggtct tttcaggtac cacagaagac ttctgcacgg
     1321 taaaagaggc cactacaatg cctgcaacaa cgatgagggc tacgagaagg taatatttct
     1381 ttgacatact tgattactgc aacaactgta aacaaaaata attccaaact ggttaaattg
     1441 caatcgaagg ggctgttaac aggtctccgg aagctatggg aagaaggatc gtgataggaa
     1501 taacaggtgc cagcggggcc atttatgcac aggtattgct ggatgccatc gccgccatga
     1561 aagagcctcc ggaagcggtt ggagtggtca tgagtagcaa tgccaaaact gtatggcagc
     1621 atgaactggg tcaggaaatg cccgattatc ccgcttttca tttctatgcc tccaaagatt
     1681 tcatggcacc tttcgccagc ggctctgctc aatatgacac gatgatcatc tgcccctgca
     1741 gcatgggcac catgggcaga atagcagcag gtatctccaa tgatctgatg acccgtgctg
     1801 cagatgtagt gctcaaggaa agaagaaaac tgatcctggt agcaagagaa acaccttaca
     1861 gcctgatcca tctgcgtaat atggagacca tcaccctggc cggtggtatc atttgtccgg
     1921 caacaccgag cttctacagc agaccgtcag atatgcgtgc gctggcagcc actgtgaccg
     1981 atcgtgtgct ggacctggcc ggtttttcaa atgcatccta ccgctgggga aactcctgat
     2041 ggtcacagtt ccgtgcatat gttaatgacc tcctgcggtt gattgctcat cttagcagca
     2101 tccaccagaa aagttaactg atgaaatttg ctgctgttct actcctttgt tccttttcat
     2161 gttctttgca agcccagacc attccagctg aacgaacggt caactggtcg ctggcaggac
     2221 tggaagcaga ttgtttcgcc gactggcctg tactgaatat catttcagcc ggcggatatg
     2281 ccgatggctc cacttccaat gatgcggtac tggcagaggt gctggacgga cttacagaac
     2341 ccaccatcat ttactttcct tctggcgatt atttatttga agaccagata gccttgcctt
     2401 caggcgtggt gctgcgtggc aatggtgctg atcacaccac actgatcttc gacctggcag
     2461 cagagaaaga tcttattgct gctaaaggca gcgccggtgc tgtaacggtc agtatcattg
     2521 gtgcacccgc caaaggaaat tcggtggtca aggtttctga cccctcctcc ttctctcccg
     2581 gcaatttcat catcgtaaac gatgatgaca ccgaactggt aaacaacacc tgggccctgg
     2641 gaaccaccgg tcagatcgtc aggatcatct ccatagcagg aaacaacctc accctgaatg
     2701 aaccactcag gcgttcgtat cctgcagatc gttcgccgta tgtacgttct atgaacatgg
     2761 ttaccgatgt aggaattgaa gcacttggaa tcagcaacct ttcacccatg agcagccagc
     2821 gcagcaacat tttctttcgc tatgtatgga acggatacgt gagttgtgtg gaaagctaca
     2881 agacaaacta tgcgcatgta gagctggaac atgccgccca ctgcgccgtt acaggaagtt
     2941 attttcagga tgcctgggct tatgatgatg gcggaaaagg ctatggcgtg ctggcacact
     3001 atgcagcagg tgccaacctg atcagcggaa acattttcaa acacctgcgt cattccatgc
     3061 tggtacaggc gggagcaaac ggcaatgtgt tttcttataa ttattcagaa gatccattct
     3121 ggaccggtac tgtattgcca tcagatgcag ccggcgatct ggtgttgcat ggtaactatc
     3181 cgtatgccaa tctgtttgaa ggtaatatcc tgcagaacct ggtggtcgat gattcacatg
     3241 gcctcaacgg cccttacaat accttctttc gcaacagggc agataactat ggcatcgtaa
     3301 tgtcaaacgc ctctgtggga aatgatatgc actttatagg aaacgaagtg accaacaccg
     3361 gtttcctgaa aggactgtat tctatatatg gatccggtca tatagaattt ggcaataata
     3421 taaaaggcac tgtaacacct gcaggcacca gtccgctacc cgaacaaacc ctgtacctgg
     3481 gcagtgttcc ttccttctac ctgacaaccg gcaactggcc tcccattggc ataccaaatg
     3541 ccatcagcag tgaagacatt gaatcacatt accggtttgt ttcaggcgat ctcacccttt
     3601 gtgccgattg cggcccttcc tgttcctatc cggaagatct gaatgctggg cctgtgctga
     3661 tcaataaagc cagcctgagc tggaccaccg taacagatgc atcgcggtat gaagtacaat
     3721 ataaaccttc gggtgccggc ccctggaata aggtcaaacc actggagaac aatgtgttgc
     3781 ttaccggcct gatgcccggc accaactatg tctggaaagt gcgcagccgt tgccctgacg
     3841 ggtggaccgc attttcgcct aacaccacct tcacaaccct ttctatgagg gcgggtgaag
     3901 gcccgaaggc agatatcttt cccaaccctg ccaccgatgc actatccatt cacctgcatg
     3961 ctgatggtgg tgtaattcag atcatgctga ccgatctgca tggccggcgg gcttacacag
     4021 aaacagtgac ccacgaaggc gggcagtgga tccattcgat ccggccgctt ccggcaccgg
     4081 gaatctatct gttacaggtg tacctgaaag atgaggtgat cacagaaaag gtagtgatcg
     4141 agtaagatca ttgaacagct gcagagagta gttctatggc tttctgcatg gcaggatcat
     4201 tccctttgat cagatcctga agctccggtg aaatctgaaa ttccggaatg atgccccgcc
     4261 ggttgtttcc cgcaggatcc tgaataacgt attgcctcct gggaatatag gccacgatgc
     4321 cggtatttgg aagatgcagc atgcgggaat cgccggaaat gatataaccg tttccaccgg
     4381 tctcctctcc tactacgatg cccctctggt agcgatcgat gactgaggta acgatggctg
     4441 aattggaaaa actgcctccg tttaccagca cggcaagctg ccctgtaaag gcatgcctat
     4501 ctgcaggatc agccagacct gtgtaggggc ctgccacatc tttcaatcgc atctggcttc
     4561 gtttgggaaa ctttgaactg accatattat atgaatacat catataaaaa ggttcattca
     4621 taatataacg gagcaacaga tagccgtttc gtatctcacc gccctgattg ttgcgaagat
     4681 ccagcaccag ccttttggtt ccggaggctt cgatctcttc aaagatctct ctgatctctc
     4741 ctttaaaata ttgcccgtaa tcacgacgca gaaccttgtt gtgaaaatca cgtatggtga
     4801 ggatggccac agaactgtcg ttggaaaact gcagggtgat accctttcca gcttccggat
     4861 ccaggtcatt ctgaggatag agttcttgtc gcttgctgat catggacggc ttgggaactc
     4921 cttccagaat gaccgtccgt gttgtacccg aatcttcctg aataaccacc gtatgggaag
     4981 acggatgtcc gtaaaagtat ccgtagaact cagcaa
//
LOCUS       DAOMAX010000201         4992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2887_length_4992_cov_4.008710, whole genome shotgun sequence.
ACCESSION   DAOMAX010000201 DAOMAX010000000
VERSION     DAOMAX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_4992_cov_4.008710"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PK742_09900"
     CDS             <1..184
                     /locus_tag="PK742_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76943.1"
                     /translation="IRQAMEEIPEEDYVLTLDQLIRSAKKRYASGTPAQRHYKLMNYL
                     LSKGYERSLITERLKP"
     gene            complement(181..3717)
                     /gene="smc"
                     /locus_tag="PK742_09905"
     CDS             complement(181..3717)
                     /gene="smc"
                     /locus_tag="PK742_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQU76944.1"
                     /translation="MKLTTLEIKGFKSFADKTVLHFNESITGVVGPNGCGKSNIVDAI
                     RWVLGEQKPTAIRTEKMENLIFNGTKARKASGLAEVSLTFENTRNLLATEFSTVTISR
                     HYYKTGESEYRINGVPCRLKDITGLFLDTGISTDSYAIIELGMLDDVLHNKDNSRRRL
                     FEQAAGISKYKIRKKETLSKLNATQVDLDRVEDILFEIENNLKELEKQARKAQRYQKL
                     KADYKANSIDLAVLTLQAHKDIYKQLANQQTYESDARISIESEIQRLEASIADEKTAL
                     IEKEQELSTLQKSMNAMLDEIRQKENLGNLNRERIKFLEERRVQLDKAIADANKTISE
                     TSGVVEELAAKAEEEQARMEALKEGVQEKLKDLDAARAVSEESRTVLEGEQRAYKQAE
                     AALYEIDKKIAINKVRLENFQREMANLSQDKTNQEAQFDPLEKQLREISETFNLAEEE
                     LSALRQQETSLQADLETANNQVEEARTALAEAHRQHDARANEYQLTRNMVDSMEGYPE
                     TIRYIRKEIASMKDAPLLGDIINPDAAHKAAIESYLEPWLNHYIVQNTAQALEVIGEL
                     DRKEKGRANFFLLNEFKTVQRVQTPSGAVSALDKVQVDERYRPLAEQLLGHVFIVPGD
                     RFAEDSARISSDHPQATIIRADGKAVRSGKTLSGGFSGVLKGNKLGVEKNLERMKTEV
                     AQLHEKVTTLSASLEKAQATSANLRQTLQALPLRQAQEKVAGLGQARVAAQSKLDHAK
                     SAISQIMEKIAGFGQMSEENKTESLQLEQDHQSQVLAKEAQHNKLIETEHAYRKTTEE
                     LDRVSDIYNKENIRYHQQQNVFQSISQELSFKRTRLEDARNTSEQNTVQLEQVISELE
                     LTKTKTEELAEELVQRYEDKKDLEATIGAKEATYYGSRGNVAETENKIKDLQKKKENS
                     DGILNDIKDRLNDLKVELAGTKERLWVEFQVELDSILDNEPPEEVSLEELRERVDANK
                     RRLENFGEVNPMAVQAYEEMKARYEFIKTQREDLLQAKESLLTTIDEIDQTARDKFEE
                     AFNQVRQNFVEVFRSLFTEDDDCDLILKDGYDPLEADVEIIAKPKGKKPQVIDQLSGG
                     EKTLTAMALLFALYLIKPAPFCILDEVDAPLDDNNIMKFNNMIRRFSDNSQFIIVTHN
                     KRTMSQVDVIYGVTMAEQGISKVVPVDFRNVA"
     gene            4021..4491
                     /locus_tag="PK742_09910"
     CDS             4021..4491
                     /locus_tag="PK742_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76945.1"
                     /translation="MFVLWIGFSIYNASKKAAAKKAADEKKKQQRTVTTQESRPQPKK
                     PEPPKPTFQELMEEIRRAAEAEAQKKAAAKREKELARELKARRIEDQEKKRHEEYMRF
                     VEKQRHEQHDAESHQLDQRIMKVYSQSRKKPALLKKLDLKHIIISDAILNRPYK"
     gene            complement(4492..>4992)
                     /locus_tag="PK742_09915"
     CDS             complement(4492..>4992)
                     /locus_tag="PK742_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQU76946.1"
                     /translation="YYGATVIHPKTIQPLRMKQIPLHVRSFLQPEGKGTVVHMDHVER
                     AYPPVLVLKKNQALLSITTRDYSFMVEEHLSQLYRIFSEHRVRINLLQTAALSLSVCV
                     DFIPEKLKPLREALSVHFKVTENTGLQLLTVRHYNQEVLDRLLGNREPLLTQKSRHTI
                     QMALPD"
BASE COUNT         1031 a         1266 c         1278 g         1417 t
ORIGIN      
        1 catccgtcag gcgatggagg aaattccgga ggaggactat gtgctcacgc tggaccagct
       61 gatccgttct gcgaagaaaa gatatgcctc aggcacaccg gcacaacgac actacaagtt
      121 gatgaattac ctgttgtcta aagggtatga gcggtcgctc attactgagc ggctcaagcc
      181 ttaagccaca ttccggaagt caaccggcac caccttgctg ataccctgct cggccatggt
      241 cacgccatag atcacgtcca cctgagacat ggtacgcttg ttgtgcgtta cgatgatgaa
      301 ctggctgttg tcgctgaacc gacggatcat gttattgaac ttcatgatgt tgttatcatc
      361 cagcggggca tctacttcat caaggataca gaacggagcc ggtttgatca ggtagagtgc
      421 gaatagcaat gccatggctg tcagggtttt ttctcctcct gataactgat cgatcacctg
      481 tggtttcttg cccttcggct tggcgatgat ctctacgtct gcctcaagcg gatcgtatcc
      541 gtctttcagt atcaggtcgc agtcgtcgtc ttctgtgaac aggcttcgga acacttccac
      601 gaaattctga cgtacctggt tgaaggcttc ctcgaattta tccctggcgg tctgatcgat
      661 ctcatcgatg gtggtgagca gtgattcttt cgcctgcaag aggtcttccc gctgggtctt
      721 gatgaattcg tagcgggctt tcatctcttc ataggcctga actgccatcg ggttcacttc
      781 cccgaagttc tccagtctgc gcttgttggc atccacccgt tcacgcagtt cttccagact
      841 tacctcttcc ggcggctcgt tgtcgagaat ggaatccagt tcaacctgga attccaccca
      901 aagacgttcc ttcgttcctg ccaactctac cttaaggtca ttcagcctgt ctttgatatc
      961 gttgaggata ccatcgctgt tctctttctt cttctgaagg tccttgatct tgttctcggt
     1021 ttctgccaca ttgccccggc tgccatagta ggtggcttcc ttggcaccga tggtggcctc
     1081 caggtctttc ttgtcttcgt agcgctgcac cagctcttct gccagttctt ccgtttttgt
     1141 tttggtcagt tccagttcac tgatgacctg ttccagttgc acggtattct gttcgctggt
     1201 attgcgggca tcttccaggc gggtgcgctt gaagctgagc tcctgcgaga tactctggaa
     1261 cacattctgt tgctggtggt agcggatgtt ctccttgttg tagatgtcgc ttacacggtc
     1321 cagttcttca gtggtcttgc ggtaggcgtg ctctgtctct atgagcttat tatgctgtgc
     1381 ttctttggcc agtacctggg actgatgatc ctgttccagt tgcagactct cggtcttgtt
     1441 ctcttcgctc atctgtccga acccggctat cttctccatg atctggctaa tggcactctt
     1501 agcgtgatcc agtttggatt gtgcggctac cctggcctga ccaagtccgg ccactttttc
     1561 ctgagcctgc ctgagcggaa gtgcctgcag ggtctgacgc aggttggcgg aagttgcctg
     1621 agctttttcc agcgatgcgg acagtgtggt tactttttca tgcagttgcg ctacctcggt
     1681 cttcattctt tcgaggttct tttctacgcc gagtttgttg cctttcagta cacctgaaaa
     1741 tccgccgcta agggtcttgc cgctgcgcac tgctttgccg tctgcacgga tgatggtggc
     1801 ctgcggatgg tcgctgctga tgcgggcgct gtcttctgcg aaccgatcgc ctggaacgat
     1861 aaagacatgt ccgagcagtt gttctgccag cggccgatag cgttcatcca cctgcacctt
     1921 gtccagcgca gatacagcac ccgaaggggt ctgcacacgc tgaacggtct tgaattcatt
     1981 gagcaggaag aaattggctc tgcctttttc cttacggtcc agttcgccga tgacctcaag
     2041 ggcctgggcg gtattctgga cgatataatg gttgagccag ggctcgagat agctttcaat
     2101 ggcagcttta tgggctgcat ccggattgat gatatcaccc aataggggag cgtctttcat
     2161 ggaggcgatc tcctttcgga tgtaccggat ggtttccggg taaccctcca tactgtccac
     2221 catgttgcgg gtaagctggt actcgttggc acgggcatca tgctggcgat gcgcctctgc
     2281 cagtgcggtc cgggcttctt ccacctggtt gttggcagtt tccaggtcgg cctgcagact
     2341 ggtttcctgc tgacgaagag ccgataattc ttcctctgcc aggttgaatg tttcactgat
     2401 ttcacggagt tgtttctcca gcggatcgaa ctgcgcctcc tggttggtct tatcctggct
     2461 cagattggcc atttcacgct ggaagttctc cagcctgacc ttgttgatgg cgatcttctt
     2521 gtcgatttcg tagagagctg cttcggcctg tttgtaggct ctttgttcgc cttccagaac
     2581 cgtgcgggat tcttcactca cagcacgggc tgcatccagg tctttcagct tttcctgcac
     2641 cccttctttc agggcttcca tcctggcctg ttcttcttcc gcttttgcag ccagttcttc
     2701 caccacaccg gatgtctctg agatggtctt gttggcatct gcaatggcct tatccagctg
     2761 taccctccgt tcttccagga atttgatgcg ctcccggttc aggttgccca gattctcttt
     2821 ctggcggatc tcatcaagca tggcgttcat gctcttctgc aaggtgctca gttcctgttc
     2881 cttttcgatc agtgctgtct tctcatcggc gatggaggct tccaggcgct ggatctctga
     2941 ctcgatggaa atgcgggcat cactctcgta agtctgctgg ttggccagct gcttgtaaat
     3001 gtctttatgt gcctgcaggg tcagtaccgc cagatcgatg ctgttggcct tgtaatcagc
     3061 cttgagcttc tggtagcgct gggcctttct ggcctgcttt tccagctctt tcaggttgtt
     3121 ctcgatctcg aagaggatgt cttccacccg gtccagatcc acctgtgtgg cattcagctt
     3181 actcagggtc tcctttttgc ggatcttgta cttactgatg cccgctgcct gttcgaacag
     3241 tctgcgccgg ctgttgtcct tgttgtggag tacatcatcc agcataccca gttcgatgat
     3301 ggcgtagctg tctgtgctga taccggtatc aaggaaaagt cctgtgatat ccttcaggcg
     3361 acagggaacg ccattgatgc ggtattcact ttcgccggtc ttatagtagt gccgactaat
     3421 agttacagta gaaaattccg tggccaggag gttgcgggtg ttttcgaagg tcagggatac
     3481 ttcggccaaa ccagaagcct ttcttgcctt ggtgccattg aagatgaggt tctccatctt
     3541 ctccgtacgg atagcagttg gcttctgttc gcccaaaacc cagcgtatgg catcaacaat
     3601 gtttgatttt ccgcagccat tgggtcctac cacaccggta atgctttcat tgaaatgcaa
     3661 caccgtctta tcggcaaaac tcttgaagcc tttgatctct agagtggtca gtttcacgcc
     3721 tgaatttttt aaatcgtgaa gacaaaaata taggaagaaa aaagtgaaaa gggaaggtgt
     3781 tattccacac ttgttgaaaa ataaacggcc agacaggagg ttgattgcag aaagaatgtt
     3841 attgataatc taatagttta aataaagttc ataaaaagtt gaaatatagt gctataagta
     3901 aatataatta gacgggtcta aatttataaa acaggattcc ggaaggcttg cagccgcata
     3961 taaatttgtt gtaccttcgg ttcagataca ggaagcatgg attctttatt tggtatatta
     4021 atgttcgtcc tctggatcgg tttcagcatt tacaatgcca gcaaaaaagc ggctgcaaaa
     4081 aaagcagctg acgaaaaaaa gaagcagcag agaacggtca ccacccagga gtccagaccc
     4141 cagcctaaga aacctgaacc gcccaagccc accttccagg aactgatgga agagatcagg
     4201 cgggctgcag aagcagaagc ccagaaaaag gcagctgcca aaagggagaa agaactggcc
     4261 cgtgagttaa aggccaggcg gatagaagat caggaaaaga aacgccatga ggagtatatg
     4321 cggttcgttg aaaagcagcg gcatgagcag catgatgcgg agtcgcacca gctggaccag
     4381 cggatcatga aggtttattc acaatcaagg aagaagcccg ctttgttgaa gaagctggat
     4441 ctgaagcata tcatcatctc tgatgcaatc ctgaaccggc cttacaagtg atcagtctgg
     4501 cagggccatc tgtatggtat gcctgctttt ctgggtcagc agtggttctc tgttgcccag
     4561 taatctgtcc agcacttcct ggttgtaatg tcttaccgtc aaaagctgta gtccggtatt
     4621 ctctgtcact ttgaaatgga ccgacaatgc ttcccgcagg ggtttgagct tttccggaat
     4681 aaagtccaca catacagaca aactcagtgc agcggtttgc agcaggttga tgcgcaccct
     4741 gtgttctgag aagatgcggt acagctgact gaggtgttcc tccaccataa agctgtagtc
     4801 gcgggtggtg atagacaaga gtgcctggtt ctttttcagc accaatacag gcggataggc
     4861 tctttccaca tgatccatat gcaccactgt tcctttgcct tccggttgca ggaaggagcg
     4921 cacatgcagc ggaatctgtt tcatgcgcag cggctggatg gtcttcggat gaatgaccgt
     4981 tgctccgtaa ta
//
LOCUS       DAOMAX010000202         4983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2896_length_4983_cov_4.399148, whole genome shotgun sequence.
ACCESSION   DAOMAX010000202 DAOMAX010000000
VERSION     DAOMAX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_4983_cov_4.399148"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK742_09920"
     CDS             complement(<1..341)
                     /locus_tag="PK742_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76947.1"
                     /translation="MVTKRSAGTIGYLCEVNSAEKPAIRDLSREELEQFFVEQGAPAF
                     RGRQVHAWLWKKHAHSFAEMSDLPSATRDMLESAFSLNHLSVDLHQKSSDGTVKVRFR
                     LYDGNLVEGVL"
     gene            373..1413
                     /locus_tag="PK742_09925"
     CDS             373..1413
                     /locus_tag="PK742_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQU76948.1"
                     /translation="MRRTSLLLVMFFLAVTGYGQDIHFSQFYAAPLNLNPSQTGMMNG
                     NLRAILNYRNQWNSFAPFTTYNGSLDVNIGHNFLDHDLIGIGVNFYNDVAGDAEFSTT
                     HASLSVSYIKTMGNKFARNYLSVGFSGGMVQRSVDYNQMTFGSQFNGYEYDPGLFSGE
                     YIGFENLSYFDLAGGITWMLVPKDQLNFYMGLAFHHLNSPDQSFTGLTSDNLYMKTTG
                     NMGAQIPVGAARNAFLVPAVMAMIQGPHYQLNGGMSVRYIINDLDYKSTAVQLGVFQR
                     VGLNEQTDYRSDATIVAARFDYLNLSVGMSYDVNVSTLQDASNAMGGPEVSLTFTSDL
                     PHRDRKVDCPKF"
     gene            1416..2066
                     /locus_tag="PK742_09930"
     CDS             1416..2066
                     /locus_tag="PK742_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76949.1"
                     /translation="MTRFSSCLFLFISGLSGVVHAQYTHSFKLEYGEVTKTDYRVQQG
                     QQVLVEYERAWTTVEDEDTIVFRETYKYPKFWRLNRYLLSDGDPKLSGWQQNFDEDGR
                     LFLEQLCQGSKKCTVSRRYTYYPGDQLMTVANYYKEALHGSHLVYYNNGQLRQHIWYE
                     NGRMQEVLAYYDDSGNPLDPGELCDGNGDVNVYSMNGSLYKIKTYRKGKARKVKEL"
     gene            2128..2307
                     /locus_tag="PK742_09935"
     CDS             2128..2307
                     /locus_tag="PK742_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76950.1"
                     /translation="MKLHKKIEPVVFWVMTIAFICNIFHLPYNTYISAVCLFMMTMYI
                     ILLVASFASKLFRRK"
     gene            complement(2419..3186)
                     /locus_tag="PK742_09940"
     CDS             complement(2419..3186)
                     /locus_tag="PK742_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQU76951.1"
                     /translation="MPLHEVINNKSVLIGRETITIDGRNIKVFTYLEKRAYSRAAIGS
                     KGVHIRLASWMSKADQQEQYKSLKQWAIQHLNKHKSLSLYKGFRTYEDGSTFEIAGKA
                     FTILRRYHSGQKSTGKLKNGVMTLHLAEGMSPAEEQEHRSYLVARMVGNAFLPEIRRR
                     LEELNVQHFQKAIGKVRLRNNTSNWGSCSYDGNISISTRLLFAPWPAIDYVLIHELAH
                     LVEHNHSDRFWKQVERAMPDYRKHEIWLDKNNHLCQF"
     gene            complement(3186..4880)
                     /locus_tag="PK742_09945"
     CDS             complement(3186..4880)
                     /locus_tag="PK742_09945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HQU76952.1"
                     /translation="MTFSEFPYTRPDLETFEQTFTEALEVFQKADSAASQIAAMEAIN
                     EVRSGFDTQMQIAMIRHTIDTRDAFYAGEQAFYDEHSPIVENLVSQYYKALVGSPFRK
                     ELEAHFGPQLFRLADMSLKTFSPEIMDLLVQENKLGTKYTEILASASIPFEGEERNLS
                     EFGPFMRSPDRDLRKRAAEARWGFLAEKRSDLEQIFHELVQVRHAMASKLGYDNFIRM
                     GYDRMLRSDYDEERSAFYREQIRKYVVPIAGQIRQEQAGRLGLDHLKYYDEALQFPDG
                     NATPNGDPDWIIAQGRKMYEDLSAETTGFIHYMLDNGLMDLVAKKGKAGGGYCTYITG
                     HNAPFIFSNFNGTSGDIDVLTHEVGHAFQVYLSKDQPTVEYYWPTSDAAEIHSMSMEF
                     FTYPFMDLFFNGKAERYRFSHTLESVLFLPYGVAVDEFQHVVYGHPDMSVEERNQAWK
                     EIEAKYLPDRDFDGFSHLSAGTWWQTQSHIYQSPFYYIDYTLAQMCAFQFWMLAKEDR
                     NAAFDRYIRLCKAGGSRSFLELVDYAGLQSPFEQGVVENVIGKVSDWIANFDRSHL"
BASE COUNT         1171 a         1255 c         1269 g         1288 t
ORIGIN      
        1 atgagcactc cttccaccag gttgccatcg tacagtctga acctgacctt gaccgttccg
       61 tcgctgcttt tttgatgcag atcaacggac aaatgattga gactgaatgc agattccagc
      121 atgtctctgg tagccgaagg caggtcgctc atttccgcaa agctgtgtgc atgtttcttc
      181 cacaaccagg catggacctg tcgcccgcga aaagcggggg cgccctgctc tacaaagaac
      241 tgctcgagtt cttccctgct aaggtcgcgg atggcgggtt tttcagcaga attcacctca
      301 caaagatagc ctatcgttcc ggctgacctt tttgttacca ttttattaat tttggtaact
      361 gatttaaacg ccatgcgacg tacctcacta ctgcttgtta tgttcttcct tgccgtaacc
      421 ggttatggac aggacattca tttctcgcag ttttatgccg caccactgaa cctgaatccc
      481 agtcagaccg gcatgatgaa cggaaacctt cgtgctattc tcaattacag gaatcagtgg
      541 aactcctttg caccttttac aacatacaat ggttcgctgg atgtgaacat cggccataat
      601 tttctggatc atgatctgat cgggatcggg gtcaattttt acaatgatgt ggccggtgat
      661 gcagaattca gcaccaccca tgcctcactg agtgtttcct atatcaagac catgggcaac
      721 aagtttgcaa ggaactacct gtctgttgga ttttccggtg gaatggtgca acgcagcgtt
      781 gactataacc aaatgacatt tggcagtcag ttcaacggtt acgaatatga cccaggtctg
      841 ttcagtggtg agtacatcgg atttgagaat ctgtcttact ttgatcttgc ggggggcatc
      901 acctggatgc tggtgccaaa agaccagctg aacttttata tgggccttgc cttccatcac
      961 ctcaactcac cggatcaaag ttttaccggt cttaccagcg acaatctgta tatgaagacc
     1021 accggaaaca tgggtgcaca gataccggtt ggcgctgcca ggaatgcatt tctggtgccc
     1081 gctgtcatgg ccatgatcca gggcccgcac taccagttga acggtgggat gagtgtgcgc
     1141 tacatcatca acgacctgga ctacaaatca actgccgttc agctgggtgt tttccagcgg
     1201 gtaggcttga atgaacagac cgattaccgt tctgacgcta caatagtagc tgccagattt
     1261 gactatctca acctgagtgt aggtatgagt tatgacgtca atgtaagtac cctgcaggac
     1321 gcctctaacg ccatgggcgg accggaagtg tcactcactt tcaccagcga cctgccccac
     1381 cgtgacagaa aagtggactg tccgaagttc tgagcatgac tcgtttttcg tcttgtttat
     1441 tcctgttcat ttcaggcctt tcaggtgtcg ttcatgctca atacacccat tcattcaagc
     1501 tggagtatgg cgaagtgacc aaaacagatt accgagtgca gcaaggacaa caggtattgg
     1561 tggagtatga aagggcgtgg actacagtag aggatgaaga caccatagtg ttcagagaaa
     1621 catacaagta ccctaagttc tggcgattga atcgctatct gttgtctgat ggtgatccga
     1681 aactgagcgg atggcagcag aactttgatg aagatggcag gctttttctt gaacagcttt
     1741 gtcagggcag caaaaaatgt acggtatcca ggaggtacac ctattacccc ggcgatcagc
     1801 tgatgaccgt ggccaattac tacaaggaag cattgcacgg atcgcatctg gtctattaca
     1861 acaacggaca attgcgacaa catatctggt atgagaacgg acgcatgcag gaagtgcttg
     1921 cctactatga tgacagcggt aacccactgg atcccggaga actgtgcgat ggcaacggcg
     1981 atgtcaatgt ctactccatg aacggatcgc tgtataagat caagacctac cggaagggta
     2041 aggcgaggaa ggtgaaggaa ctttagtcag ttggcagttc tcaggctgaa gtccggagtc
     2101 catttcattt ggaaatgcca atgtaaaatg aaattacaca agaagataga accggttgta
     2161 ttctgggtga tgacgatcgc attcatctgc aacatctttc atctgccata caacacgtat
     2221 atcagtgcgg tctgtttgtt catgatgacc atgtacatca ttctgcttgt cgcatctttc
     2281 gccagcaagc tgtttcgccg gaagtgattg cttctacctt ccacttttcc ctctcttcaa
     2341 ccgcaaaggc gccaggatgc aaaggcgcaa aggattccag tacttatggc ttcagaccaa
     2401 agactaacct tttctctctc agaattgaca caaatgattg ttcttatcca gccagatctc
     2461 gtgtttgcga tagtcgggca tggcccgttc cacctgtttc cagaagcgat ctgaatggtt
     2521 gtgttccacc aggtgcgcca gctcatgaat gagtacataa tcgatggcgg gccagggggc
     2581 gaagagcaat ctcgtgctga tgctgatatt accatcatag gaacaactgc cccagttgga
     2641 ggtgttgttg cgcagcctca ccttgccgat ggctttctgg aaatgctgca cgtttagttc
     2701 ttccagtcgg cggcggatct ctggcaagaa tgcattaccc accatccgtg ccaccaggta
     2761 actgcggtgt tcctgttctt cagcgggcga cataccctcc gccaggtgga gggtcatgac
     2821 cccgttcttc agtttgcccg tgcttttttg tccgctgtgg tatctacgca gaatggtgaa
     2881 ggccttccct gcaatttcga aggtgctgcc gtcttcatag gtgcgaaatc ctttatacag
     2941 cgataaggac ttgtgtttgt tcaggtgctg gatggcccat tgcttcaggc tcttatactg
     3001 ctcctgctga tcagccttgc tcatccagct ggcgaggcgg atgtgtacac ctttgctgcc
     3061 gatggctgcc cggctgtagg cacgcttttc cagataggtg aataccttga tattcctccc
     3121 gtcgatggtg atggtctcgc ggccaatgag gacggacttg ttgttgatga cttcgtgcag
     3181 cggcattaca ggtgcgacct gtcaaagttg gcgatccagt cgcttacttt acctatcacg
     3241 ttttccacga ctccctgttc aaacggcgac tgcaacccgg cgtaatccac cagctccagg
     3301 aaggaacggc tgcctccggc cttgcagagt ctgatgtagc ggtcaaaggc tgcattgcgg
     3361 tcttcctttg ccagcatcca gaactggaag gcacacatct gcgccagcgt ataatcgatg
     3421 tagtagaacg gactctggta aatatgcgat tgtgtctgcc accaggtgcc tgcactaagg
     3481 tgactaaaac catcaaagtc gcggtctggc aaatactttg cttctatctc tttccatgcc
     3541 tggttgcgct cttccacgct catatcagga tggccgtaca cgacgtgctg gaattcatcc
     3601 actgccacac catagggtaa gaacaacaca gactccagtg tatgcgaaaa ccggtagcgt
     3661 tctgccttgc cattgaaaaa cagatccatg aagggatagg tgaagaactc catcgacatg
     3721 gaatggatct cggccgcatc actcgtgggc cagtagtatt ccaccgtagg ctggtccttg
     3781 ctcaggtaca cctgaaacgc atgacctact tcatgggtga gcacgtcaat atcgccagat
     3841 gtaccattga aattggagaa gatgaacgga gcgttgtgtc cggtgatgta agtgcaatac
     3901 ccaccacctg cttttccttt cttcgccacc aggtccatca gtccgttgtc cagcatatag
     3961 tggatgaagc cggtggtctc tgctgacagg tcctcataca tctttcttcc ctgtgcgatg
     4021 atccagtcgg gatcgccatt gggagtggcg ttaccatcgg ggaactgcag ggcttcatcg
     4081 tagtacttca gatgatccag gcccagtctt ccggcttgtt cctgcctgat ctgtccggcg
     4141 atgggtacca catattttct gatctgctcc cggtagaaag cagaccgttc ttcatcgtaa
     4201 tcgctgcgca gcatgcggtc gtagcccatg cggatgaagt tgtcataccc cagcttgctg
     4261 gccatggcat gacgcacctg caccagctcg tggaagatct gttccaggtc actgcgcttc
     4321 tctgccagga aaccccagcg tgcttctgct gcccgcttac gaagatcgcg gtcgggactg
     4381 cgcatgaagg gaccgaactc actcaggttg cgttcttcac cttcaaaggg gatgcttgca
     4441 gaagccagta tctctgtgta tttggtaccg agtttattct cctgcacaag gaggtccatg
     4501 atctccggcg aaaaggtttt caaagacata tctgccagac ggaacagttg cggaccaaaa
     4561 tgcgcttcca gttcctttct gaaaggcgag ccgaccagtg ctttataata ctggctgacc
     4621 aggttctcca cgatgggcga atgttcatcg tagaatgcct gttcaccggc atagaatgca
     4681 tcgcgggtgt cgatggtatg gcggatcatg gcgatctgca tctgcgtatc aaaaccggag
     4741 cgcacctcgt tgatcgcttc catggcggct atctgcgagg cagcagaatc agctttttga
     4801 aatacttcta atgcttcagt gaacgtctgc tcaaaagttt cgagatcggg acgtgtgtag
     4861 ggaaattcgg agaaagtcac ggggcaaaga taatggatgg gggagcagtg gaggagtgga
     4921 gcagtgagca gtggaggagt tggcaatgga tctacgaatt cccttccagc gtacccacag
     4981 agt
//
LOCUS       DAOMAX010000203         4943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2928_length_4943_cov_3.943331, whole genome shotgun sequence.
ACCESSION   DAOMAX010000203 DAOMAX010000000
VERSION     DAOMAX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_4943_cov_3.943331"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /gene="ychF"
                     /locus_tag="PK742_09950"
     CDS             complement(<1..689)
                     /gene="ychF"
                     /locus_tag="PK742_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQU76953.1"
                     /translation="MGLQCGIVGLPNVGKSTLFNALSNAKAQAANFPFCTIEPNVGVV
                     TVPDQRLNKLAELVQPQNLLPTSVEFVDIAGLVKGASKGEGLGNKFLANIRETDAIIH
                     VVRCFENDNIVHVDGSVDPVRDKEIIDFELQLKDLETLDSRIAKTQRAAGTGDKEAKK
                     LMEVMSACKSHLEQGKSARSLQLDDDGKKTLDSLYLLTTKPVIYVCNVEEKNVLKGNE
                     HVERLKAAVAD"
     gene            815..1711
                     /locus_tag="PK742_09955"
     CDS             815..1711
                     /locus_tag="PK742_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76954.1"
                     /translation="MRKPSAILILVLSGILLTVSCARDEILHENLVVDGNTPPPSNGI
                     STLQLNNYINNLYIDLFGRAPTEGELEDRSEYLRTNNYSEEARGTIIDELMESYEYYK
                     NINILTTQKMLVDVDSITVTNQIATYEYIIYLNELAGDTLYNYLIEFEVQKLTDLLQA
                     PVDLYEGSIGVNEYFRRFIYNYYYDEVNMGSLNFVVSCFENLFQRLPTDEETSDGIAM
                     VDGTSSQLLLIDGSSKEDFVDIVTNHLEFYQGQVIEAYHTLLAREPSSFEMYADGLDM
                     MSDNHFNAVKKKILQSEEYAGF"
     gene            1698..2216
                     /locus_tag="PK742_09960"
     CDS             1698..2216
                     /locus_tag="PK742_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76955.1"
                     /translation="MQVSKWIVGALAVTLFWSSCKKEITQEIIYDNIIYQIDTVPVYD
                     SNLEKDRLKTPLQFISSVFSNLYFSSIPSSVLDNLVVYRLSVGDKSLVNELIINAMLQ
                     DPVVLANIPTDEEMRLDIDDFIFTTYLRFYLRYPTEYEKYELKNMIEENTDLDPVEIF
                     RAFLLSNEYQFY"
     gene            2216..3544
                     /locus_tag="PK742_09965"
     CDS             2216..3544
                     /locus_tag="PK742_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76956.1"
                     /translation="MKRRKFIRNLSMAAAGAIAAPYILPSGRLFAASGSRLVNHVVLV
                     LFGGGIRNQESVEQQYLLSQGAGPGGNVMRNMLNGAAPPYALVYEPWTPILPEPLANS
                     GTLFKHMRYEQGPTGHFNGHSAVLTGHYNETSLNLNINPEYPTVFEYYRKHSSPARNA
                     VNAWWLSMDLGPYPALNYSRHPDYGPNYGANYISPLTTFGGIGYDYFSTLNTLHPEEL
                     ARLNDLRSFLDSNFDKSITDIPGIVNTAEDRAEIQQFIIDLLNGSIPVEYPLPGGVSP
                     DQISGDLINITAAWQVLNRFEPELLVINTTNLDICHSDFSSYLSLLHRADYGVGWLWD
                     KILSHPVLANDTIMICIPEHGRNAEPNAILDSNGLFAYDHTSDENSRDIFGLIVGPAG
                     IVNQGHVIGSPEEAAGQAIDIVPTIAHILGFHDELPPFMLSGSVLEGAFV"
     gene            3541..4527
                     /locus_tag="PK742_09970"
     CDS             3541..4527
                     /locus_tag="PK742_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76957.1"
                     /translation="MRTRIVIPGMLLGTFLAVAGCKTEFIRLEPDNLPLNPFDTIDYG
                     EEMLEVLDIDSASFLGLHTYIFQPTCAVPGCHDGNFEPDFRTVQSSYNTLLYAPVIKN
                     DDAGTFTYRVIPGDTALSWLHERITTDDEVLGRMPLYDTLYPAEREKITTWILDGAKD
                     VMGNSPSLPNYQPVSFGFIAYENDTLGIRLDENRADAVSPIGLPDNTMVEFWFGVYDT
                     DEYGSYLGGWDLDYNKVRISKEPYFFTDPAEYSMEVLDALTPYIGPLFWDPLVPVPYY
                     HHFTINTADFDPGSYYFMRFYVDDGDHPEPTEIPNTGSPSYLLTYFSFYIQE"
     gene            4524..>4943
                     /locus_tag="PK742_09975"
     CDS             4524..>4943
                     /locus_tag="PK742_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76958.1"
                     /translation="MRQISYIVLSCLMLGITACKKETVSEQPPISPVPSIELRNTAPT
                     VVTEYEDSIVFDLFYLDGDGDLGFADPDSTSVFLVDTRIQTVEDFYLPLLAPENVSVP
                     IQGIFQLHLDRTILTGSGDTPETVVFQIYLRDRAGHQS"
BASE COUNT         1248 a         1255 c         1141 g         1299 t
ORIGIN      
        1 tcatcggcca ctgcggcctt cagcctttct acatgctcat tacccttcag aacattcttc
       61 tcttctacat tacaaacgta tataaccggc ttggtggtaa gcagataaag actgtccagc
      121 gttttcttac cgtcatcatc caactgcagg cttctggcac tcttcccctg ttcgagatgc
      181 gatttacagg ccgacatcac ctccataagt ttcttcgctt ccttatcgcc ggtacctgct
      241 gcacgttggg tcttggcgat cctggaatcc agtgtttcca gatccttcag ttgcagctca
      301 aagtctatga tctctttgtc tcgaacagga tccacacttc cgtccacatg gacgatgttg
      361 tcattttcaa aacaccttac cacatggatg atggcatcgg tctcacggat attggccaaa
      421 aatttattcc ccagcccttc tcccttactg gcccctttca ccagaccggc tatatcaaca
      481 aattcaactg aagtaggcag gagattttga ggctgaacca attctgccag tttgttgagt
      541 cgctgatcag gcactgttac cacacccaca ttaggctcaa tggtacaaaa agggaaattg
      601 gccgcctgtg ccttggcatt actaagggcg ttgaacaagg tcgattttcc cacattcggc
      661 aggcctacta ttccgcattg aagtcccata ttctctgttt aaacggccgc aaagatacgt
      721 catctgcttg cgcaccggaa caaaatgatt aatttaggta cggaaattgt taaaccaaac
      781 gttatgctat cggtcattcc gatggtcggt taatatgcgt aaaccctctg ccatactgat
      841 ccttgtactt tccggcatcc tgttgacggt ttcctgtgcc agggatgaga tcctgcatga
      901 aaaccttgtg gtggacggaa acacgcctcc tccatccaac ggcatctcca ccctccagct
      961 caataattat atcaacaacc tgtacataga tctgtttggc cgtgctccca ccgaaggaga
     1021 actggaagac cggagtgaat acctccgtac caataactat agtgaagaag cccgtggcac
     1081 catcattgat gagctgatgg aatcttatga atactataag aacatcaaca tcctgaccac
     1141 ccagaaaatg cttgttgatg tggactcgat cacggttacc aatcagatcg ccacatacga
     1201 atacattatt tatctgaatg aactggccgg tgatacgctg tataattatc tgatcgaatt
     1261 cgaagtgcag aagctgaccg atctgctgca ggctccggta gatctgtatg aaggaagcat
     1321 tggtgtcaac gaatatttca gacgcttcat ctataactat tattacgatg aggtaaacat
     1381 gggcagtctg aattttgtag tatcctgttt tgaaaacctg ttccagcgtt tgcccaccga
     1441 tgaagagacc agtgatggca ttgccatggt agatggtaca tccagccagc tgctgctcat
     1501 tgacggcagc tccaaagaag attttgttga catagtcacc aaccacctgg agttctacca
     1561 ggggcaggta attgaagctt atcataccct gcttgccaga gaaccgagct cctttgagat
     1621 gtatgcagac gggttggata tgatgagcga taaccatttc aatgctgtca aaaagaagat
     1681 ccttcaatct gaagaatatg caggtttcta agtggatagt cggcgcactg gcggtcacgc
     1741 tgttctggtc ttcctgtaaa aaggagatca cacaggagat catttatgat aacatcattt
     1801 accagatcga taccgtgcct gtctatgatt ccaatctgga aaaagacaga ctcaaaactc
     1861 ctctgcagtt catcagcagt gtgttcagta atctttactt ctccagcatc ccatccagtg
     1921 tgctggacaa cctggtggtg taccgcttgt cagttggcga caagagtctg gtgaacgagc
     1981 tgatcatcaa tgccatgttg caggatcctg tggtacttgc caatattcct actgacgagg
     2041 agatgcgact tgatatagat gatttcatct ttaccaccta cctccggttt tacctgcgat
     2101 atcctaccga atatgagaaa tatgagctga agaatatgat cgaagaaaac accgatcttg
     2161 atccggtaga aattttccgt gccttcctat taagtaacga ataccagttc tattgatgaa
     2221 aagaaggaag tttatcagga acctgtctat ggcagctgcc ggggcgatag cagctcccta
     2281 tattctgccc tccggccgat tgtttgctgc ttcgggcagc cggcttgtca accatgtagt
     2341 gctggtattg tttggcggtg gcatccgcaa ccaggaaagt gtggaacagc aatacctgct
     2401 gagtcagggt gcaggccccg gtggaaatgt aatgcgcaat atgctcaatg gtgcggcacc
     2461 accttatgca ctggtatatg aaccatggac accgatactt cctgaaccat tggccaacag
     2521 tggtaccctg ttcaaacaca tgcgctatga acaaggccct actggtcact tcaacgggca
     2581 tagtgcagta ctaacaggtc attacaacga gaccagtctg aacctgaaca tcaatccgga
     2641 ataccccacc gtatttgaat attaccggaa gcattcatcg cctgccagga atgctgtcaa
     2701 tgcctggtgg ctgtctatgg acctggggcc ttacccggcg ctgaattaca gtcgccaccc
     2761 ggattacgga ccgaactatg gtgctaatta tatcagtccg ctcaccacat tcggaggcat
     2821 cggatatgat tatttcagta ccctgaacac cttgcatccg gaagaactgg ccaggttgaa
     2881 tgacctgcgc agtttccttg acagcaactt tgacaaatcc atcactgata taccgggcat
     2941 tgtcaatacc gccgaagacc gtgctgagat ccagcagttc atcattgacc tgctgaatgg
     3001 cagcatcccg gtagaatatc cgttacccgg tggtgtgagc cctgatcaga tcagcggcga
     3061 cctgatcaac attaccgccg cctggcaggt gctgaacagg ttcgaacccg agttgctggt
     3121 catcaacacg accaatctgg acatatgcca ttctgatttc agttcctacc tgagtttgct
     3181 gcaccgtgct gactatggtg ttggctggtt gtgggacaag atcctgagtc acccggtgct
     3241 ggccaatgac accatcatga tctgcatacc tgagcatggc agaaacgcag aacccaatgc
     3301 catcctggac agcaacggtt tgtttgccta tgaccatacc agtgatgaga acagccggga
     3361 tattttcgga ctgatcgttg gccctgcggg tattgtaaac cagggtcatg tgatcggctc
     3421 gcctgaagaa gcagcaggtc aggccattga catagtaccc accatcgccc atattctggg
     3481 ctttcatgac gaacttccgc cattcatgtt gagcggcagc gtgctggagg gcgcctttgt
     3541 atgagaacaa ggatagtaat accgggtatg ctgctgggca cttttctggc ggttgcagga
     3601 tgcaaaactg aattcatacg cctggagccc gacaatcttc cgctgaatcc ttttgatacg
     3661 atcgattacg gcgaagaaat gctggaggta ctggacatag acagtgcatc ttttttagga
     3721 ctgcacacct acatctttca gcctacctgt gccgttccgg gttgtcatga cggcaacttt
     3781 gaaccggact tccgcaccgt tcagtcatcc tacaatacct tgctctatgc accggtcatc
     3841 aagaatgatg atgccggcac ttttacctac cgggtaatac cgggcgatac tgccctgtcc
     3901 tggcttcatg aacgcatcac taccgatgat gaggttctgg gcagaatgcc tttgtatgac
     3961 accctgtatc cggcagaacg cgaaaagatc accacctgga tactggacgg ggcaaaagat
     4021 gtaatgggaa attcaccctc ccttcccaac taccagcctg tatcattcgg tttcatcgcc
     4081 tatgaaaatg atacgctggg catcaggctg gatgagaacc gtgcagatgc cgtttcaccc
     4141 attggcctgc cagataatac catggttgaa ttctggttcg gggtttatga tacggatgag
     4201 tacggcagct atctgggagg atgggatctg gactacaata aagttagaat cagcaaggaa
     4261 ccatactttt ttacagatcc tgcagagtat tctatggagg tactggatgc cctgacgcct
     4321 tatataggcc ctttgttctg ggatccgctg gtgccggtac cttactacca tcatttcact
     4381 atcaatactg cagattttga ccctggcagc tattacttca tgcggtttta tgttgatgac
     4441 ggcgatcatc ctgaacccac agaaattccg aatacgggtt ctccttccta tctgctcacc
     4501 tatttcagct tttacataca ggaatgaggc agatcagtta catagtattg tcatgcctga
     4561 tgctgggcat cactgcctgt aaaaaggaaa cggtatcaga acaaccacct atctctcctg
     4621 ttccgtcaat tgaactcagg aatacggcac caacggtggt aactgaatat gaagattcca
     4681 tcgtattcga cctgttctat ctggacggtg atggcgatct cggctttgcc gatcccgaca
     4741 gtaccagtgt gtttctggtg gatacgcgaa ttcagacggt cgaagatttc tacctgccat
     4801 tgcttgcacc ggagaatgtt tcggtgccca ttcagggcat ctttcaactg cacctggacc
     4861 gcaccatact cacgggtagt ggagatactc ctgaaacggt ggtctttcag atctacctcc
     4921 gcgaccgtgc aggtcatcag agc
//
LOCUS       DAOMAX010000204         4941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_2929_length_4941_cov_4.895211, whole genome shotgun sequence.
ACCESSION   DAOMAX010000204 DAOMAX010000000
VERSION     DAOMAX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2929_length_4941_cov_4.895211"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK742_09980"
     CDS             <1..611
                     /locus_tag="PK742_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76959.1"
                     /translation="QKLIAPFKDRIHTGLGVTGVLELDAGAELKLSDGSSRVFDKVIL
                     ATHADQALRLLQQPTELQRNLLLPFQYQVNDTILHTDPALMPARKRAWSSWNYRVEKA
                     DGSGGSCTYWMNSLQGVSDTTNYFVTLNGRAKIDPEKILRSYSYEHPVFTTGAMKAQE
                     QLPLLNSRGSILFCGSYFGNGFHEDAIRSAKDAVQQIVDIKW"
     gene            605..1393
                     /locus_tag="PK742_09985"
     CDS             605..1393
                     /locus_tag="PK742_09985"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HQU76960.1"
                     /translation="MVNRQSAIYHCQVMHQRMHPKRHRFSYGVYMVWLDLDEVDELCR
                     QIPWLSKNRFNLFSLFDSDHVKAAGQQGLSISAKLRNWLSEQDFEGATDKIFLMTHLR
                     VLGHVFNPVSFYYCYDSSGNCTCIVAEVSNTFGEMKMFLIDQVSELGFEQEADKFFYV
                     SPFTELDDRFHFRFHLPGQQYFTAIDVSRAGQKYFYSVLRGDRKVLNSAVLLGYFFRM
                     PLVTVKVITAIHWQAFRLWTRGLSYRKKAADPDKQKGILNEKRM"
     gene            1396..2613
                     /locus_tag="PK742_09990"
     CDS             1396..2613
                     /locus_tag="PK742_09990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQU76961.1"
                     /translation="MWNNVYRRLLLRTLNKMNKGQLTLTLPDKEVIRIGDGLGIHATL
                     QVLDEALWRRIVLHGDIGFAEGYMDRQWETDDLTALLRWVLLNIDQAPSVSGSGNASM
                     FMNLMHGFNRLTHILRPNSKKGSRKNISEHYDLSNDFFSLFLDKTMTYSSAIFQNGEE
                     DLETAQMNKYDSLCRSANLKDGDHVLEIGTGWGGFAEFAASRYKVRITTVTISKEQYD
                     FATRRIEKAGLADRVEVLLCDYRDISGSYDKVVSIEMIEAVGARYLPVYFRKIQQVLK
                     PDGVLALQAIVCPDNRFDLLKNRVDFIQKHIFPGSLLPSIAAINQAVNDTGDLFLFGL
                     KDLGKSYVRTLAAWRNAFEEHAEDVSRMGFNEVFQRKWKYYFSYCEAAFDMRNINVVQ
                     MIYTRPNNFLFGQ"
     gene            2824..4542
                     /locus_tag="PK742_09995"
     CDS             2824..4542
                     /locus_tag="PK742_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HQU76962.1"
                     /translation="MKQILFSSLMMCGLAGSLMAQTARVQVIHNAADPSAARVDVYLN
                     GDLALNDFQFRTATPYIDVPAETPITISIAPYTSVSVDDAIANFDYTLAADETYVVVA
                     NGVLDPGSFSENPDGVSTAFNLFVYAGAREAALSSENIDFIFFHGATDAPGVDAIARN
                     VATVVDDASYTDFQGYVSVPAGKYLIDVTPEDDNETIVTTARAWLPGAGGASVVIFAS
                     GFLSPEDEADNARGFKLMYALPDGTVKTLPALTSAPVQIIHNAADPAAAEVDVYVNGA
                     LALDNFAFRTATPYVALPANQDVEIAVAPGTSTSVADALATFTANFKAGEGYTVIANG
                     VLNPDDFAANPDGLSTAFNLWIREGAMGKAEGTDVVSFIGVHGSTDAPTVDIIARDVA
                     TLLDNVAYGAVSDYITVPADNYILDVALGDMPETVVVSYSADLSTLGGGSAVVFASGF
                     LDPSAGPDGSEGFGLYATLADGTTIALPVYTELTTADASRMANLVESLFPNPATDNLQ
                     VTLSEAFDGMLLLTSADGRLVLNQQVNLETAGSVQLDLSNVAAGYYMLQMRNNSGTTT
                     MPVIVR"
     gene            complement(4596..>4941)
                     /locus_tag="PK742_10000"
     CDS             complement(4596..>4941)
                     /locus_tag="PK742_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU76963.1"
                     /translation="DEIAIARILNLLDAYKVTLHVTHFSESRHDEDAFKLHQLEEKLR
                     QDHKSERIRYEVVDSRVLLSAMKEYIDSNDIDLLALTTRKLSGLQRIFTRSTAIDVLY
                     QTNKPLLVFHEK"
BASE COUNT         1284 a         1050 c         1218 g         1389 t
ORIGIN      
        1 aacagaaact aatagcacca ttcaaagacc ggatccatac cggattgggt gtaaccggtg
       61 tgctggagct tgatgcaggt gcagaattga aattatctga tggaagcagc agggtctttg
      121 acaaggtgat cctggccaca catgcagatc aggcattgcg gctgctgcaa caaccaacag
      181 aactgcagcg caatttattg ttgccgtttc aataccaggt taacgatacc attctgcata
      241 cagatccggc acttatgcct gcccggaaac gggcctggag cagttggaat taccgggtag
      301 agaaggctga tggatccggt ggttcatgca cctactggat gaacagcctg cagggtgttt
      361 cagatacgac caactatttt gttactttaa atggcagggc gaagatcgat cctgagaaaa
      421 tacttcgttc atattcttat gagcacccgg tgtttacgac cggggccatg aaggcacagg
      481 aacagttgcc actactgaac agcaggggca gtatcctttt ttgcggcagt tactttggca
      541 atggatttca cgaagatgcg atcagaagtg caaaagacgc tgttcagcaa atagttgaca
      601 taaaatggtg aacaggcagt ctgccatata tcattgtcag gtgatgcatc agcgcatgca
      661 tccgaagaga catcggttct cctacggagt atatatggtt tggcttgatc tggatgaggt
      721 tgatgagtta tgcaggcaga tcccctggct ttctaaaaac cggtttaatc ttttttctct
      781 gtttgactct gatcatgtca aggcagcagg gcagcagggt ctgtccattt ctgcaaagct
      841 cagaaactgg ctcagtgaac aggactttga aggcgcaaca gataagatct ttctgatgac
      901 ccatctccgg gtactggggc atgtgttcaa tccggtgtca ttctattatt gctatgacag
      961 ttcgggaaac tgtacctgta tcgtggcgga ggtaagcaac acgtttggcg aaatgaaaat
     1021 gttcctgatc gatcaggtca gtgaattggg atttgagcag gaggcggata aattttttta
     1081 tgtatcgccg ttcacagagc ttgatgacag gtttcatttc agatttcact tgcctggaca
     1141 gcagtatttt actgctattg atgtaagcag agcaggtcag aaatattttt acagtgtctt
     1201 acgcggtgat cgcaaagtat tgaacagcgc tgtgctgctt ggttatttct ttcgtatgcc
     1261 acttgtcacg gtcaaggtga tcacggctat ccactggcag gctttcaggt tgtggaccag
     1321 ggggttatca tacagaaaga aggctgccga tcctgataaa caaaaaggaa tactcaatga
     1381 aaaaagaatg taagcatgtg gaataatgta tatcgcagat tacttctgcg aacactgaat
     1441 aaaatgaata agggtcagtt aacgcttact cttcctgaca aggaggtcat caggataggt
     1501 gatggtcttg gtattcatgc taccctgcag gtattggatg aggcattatg gcgaaggata
     1561 gtattgcatg gtgatattgg ctttgccgaa ggttatatgg acaggcaatg ggaaacagat
     1621 gacctgacag cacttttacg ttgggtgctg ctgaatatag atcaggctcc gtcagtaagt
     1681 ggttccggca atgcttccat gttcatgaat ctgatgcacg gcttcaatcg tctcactcat
     1741 atactccggc caaacagtaa gaaagggtcc agaaagaaca tcagtgaaca ctatgatctg
     1801 agcaatgatt tcttttcgct ttttctggac aagacaatga cctatagcag cgccattttt
     1861 cagaacggtg aagaagacct ggaaacagca caaatgaaca agtatgacag cctttgcaga
     1921 tctgccaacc ttaaagacgg agatcacgtg ctggagatcg gaaccggttg gggtggcttt
     1981 gcagagtttg ctgcttccag atacaaagtt cgtattacca cagtgaccat ttcaaaagaa
     2041 cagtatgatt ttgccaccag gcgcatagag aaggcagggc tggctgacag ggtagaagta
     2101 ttgctctgtg attacagaga cataagtggt tcgtatgaca aggtggtatc tatagagatg
     2161 atagaagctg taggtgccag gtaccttcct gtttacttca gaaagatcca gcaggtgctc
     2221 aaaccggatg gagtgctggc tttgcaggcg atcgtttgtc cggataatcg gttcgatctg
     2281 ctgaaaaatc gtgtggactt cattcagaaa catatttttc cgggaagttt gctgccttcc
     2341 attgcagcta tcaaccaggc tgtcaatgat accggcgatc tgtttttatt tggccttaaa
     2401 gatctgggaa agagctatgt acgaacactg gcggcctgga gaaatgcttt tgaagagcat
     2461 gcagaagacg tttcacgaat gggatttaac gaggtgtttc aacgtaaatg gaaatactat
     2521 ttttcttatt gcgaggctgc ctttgatatg cgcaatatca atgtggttca aatgatctat
     2581 acacgcccaa ataatttttt gtttggccag tgaacaactt tctgatcttt gctgttagtg
     2641 accttcgtct ttattttaag agtaaaatag aaaaaggtat aatacataaa aattcttttt
     2701 tagtgcaata tattgatttt cagttcactg aattttgata ctaaatgcct tgcaaaagaa
     2761 tgacagatct gtaaacgaac aaagcagcag gtatgcagtt tagtctgcaa aaccaattgc
     2821 attatgaaac agatcttatt ctcctcctta atgatgtgcg gactggcggg aagcctgatg
     2881 gcacaaaccg caagagttca ggtcattcac aatgctgctg acccttcagc ggctcgtgtg
     2941 gatgtgtatt tgaacggcga ccttgctctg aatgattttc agtttcgcac tgccacacct
     3001 tacatagatg tgcctgctga aaccccgatc accatttcca ttgcaccata cacttctgtt
     3061 agtgtggatg atgcaattgc caattttgat tacacactag ccgcagatga aacctatgtg
     3121 gttgttgcca atggtgttct cgatcctgga tccttctcag aaaatcctga tggggtatca
     3181 actgccttca acctgttcgt ttatgctggc gcacgtgaag cagcactaag ttcagagaac
     3241 attgatttta ttttctttca cggagctact gatgcaccgg gtgtagatgc cattgcccga
     3301 aatgtggcta ccgttgtaga tgatgcttct tacactgatt ttcagggtta tgtaagtgtt
     3361 cctgccggaa agtatctcat agatgtaact ccggaagacg ataatgaaac gatcgttact
     3421 actgccaggg catggctgcc tggtgcaggc ggcgcttctg tggtgatctt tgccagtgga
     3481 ttcctgagtc cggaagatga agcagacaac gcccgtggat tcaaactcat gtatgcactt
     3541 cctgatggaa ctgtaaaaac gcttcctgct ctgacctctg ctccggttca gatcattcac
     3601 aatgctgcag accctgctgc tgcagaagtg gatgtgtatg tgaatggtgc actggcactt
     3661 gacaactttg ccttccgcac cgccactcct tatgtggctc ttcctgccaa tcaggatgtg
     3721 gagatcgctg tggctcccgg taccagcaca tctgtagctg atgcactggc tacatttact
     3781 gccaatttca aagcaggcga aggctacacc gttattgcca atggtgtgct gaaccctgat
     3841 gattttgcgg ccaaccctga tggtctttca accgcattta acctgtggat acgggaaggt
     3901 gcaatgggta aggctgaagg aactgatgta gttagcttca tcggagtaca cggcagtacc
     3961 gatgccccca cagtagatat cattgcccgc gatgttgcta ctctgctgga taatgtggcc
     4021 tatggtgcag tgagtgatta tatcactgtt cctgccgata actacatcct tgatgtagca
     4081 ctgggagata tgcctgaaac agtagtggtg agttattctg ctgatctgag tacactgggc
     4141 ggaggttcag cagtggtctt tgccagcggt ttcctggatc cttcggcagg tcctgacgga
     4201 agtgaaggct tcggtctgta tgctacactg gctgatggta ccactatagc tctgcctgtt
     4261 tacacagaat tgacaactgc tgatgcgtct cgtatggcca atctggtaga aagtttgttc
     4321 ccgaatcctg caacagacaa ccttcaggta accctttctg aagcatttga tggtatgctg
     4381 ctgcttactt cggcagatgg acgtctggtg ctcaatcagc aggtaaacct ggaaacagcc
     4441 ggatctgtac agttagatct gagcaacgta gctgcaggtt attatatgct gcaaatgcgc
     4501 aacaactcag gtactaccac tatgcctgta attgttcgct gatcaatatt ttctcttcat
     4561 actaccaact gtggcggcac gtctttgacg tgccgctatt tttcgtggaa gaccaatagc
     4621 ggtttattag tttgatacag cacatcaatg gccgtgctgc gggtgaaaat gcgttgaaga
     4681 ccacttagtt ttctggtagt cagtgccagc aggtctatat cattactatc aatatactct
     4741 ttcatggctg acaggagtac ccggctgtca actacttcgt agcggatacg ttcagacttg
     4801 tgatcctgcc tcagtttttc ttccagctga tgcagtttaa aggcatcttc atcatgtctg
     4861 gattcactga aatgggtcac atgcagggta accttataag catccagcaa attgagtatc
     4921 ctggcaatgg ctatttcatc c
//
LOCUS       DAOMAX010000205         4809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3044_length_4809_cov_3.999159, whole genome shotgun sequence.
ACCESSION   DAOMAX010000205 DAOMAX010000000
VERSION     DAOMAX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3044_length_4809_cov_3.999159"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /gene="ccoO"
                     /locus_tag="PK742_10005"
     CDS             <1..1733
                     /gene="ccoO"
                     /locus_tag="PK742_10005"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQU76964.1"
                     /translation="VVFGINMFGTIMKRRERHIYVAIWFYIATFLTVAMLHIVNSIAL
                     PVNFLKSYPVYAGVQDALVQWWYGHNAVAFFLTTPYLGLMYYFLPKAANRPVYSYQLS
                     IVHFWALIFLYIWAGPHHLIYTSLPDWAQTLGVVFSFMLIAPSWGGMVNGLLTLRGAW
                     DKVRDDVVLKFMVVAVSAYGMSTFEGPMLSLRNINAWAHFTDWIVAHVHVGALGWNGF
                     LTFGILYWLVPRMWKTNLYSKSLANTHFWIGTLGIIFYAVPMYWAGWMEGSMWKQFND
                     DGMLQYPTFIETTQYVKPFYAMRSIGGVFYLTGVFIMAYNLVKTARQGSFMASEEAEA
                     APMEKVVKVYKGEFWHRALERRPIQFGMLALVAVLIGGIVELVPTFLVKSNVPTIASV
                     QPYTPLELQGRDIYVREGCYTCHSQMVRPFRNEVVRYKGEYSKAGEFVYDHPFQWGSK
                     RTGPDLHREGGKQTNAWHYWHMYEPTSMTSGSIMPSYAWLHERTLDISTTPAKIRVMQ
                     NLGVPYPEGYDAIANDDLKFQAEQIAAKLAQEDSRIKTSADKEIIALIAYLQRLGTDI
                     KAEPTAQAAN"
     gene            1739..1939
                     /locus_tag="PK742_10010"
     CDS             1739..1939
                     /locus_tag="PK742_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HQU76965.1"
                     /translation="MSFIHYLEGISGIGIYPMFSLLVFFIFFVVLLFLVWKMNRSTIE
                     ELKQIPLQDQELASDHTTTQNH"
     gene            1941..3098
                     /locus_tag="PK742_10015"
     CDS             1941..3098
                     /locus_tag="PK742_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQU76966.1"
                     /translation="MKTGNRNILRLLSLAGLTAFSTSLFAQNAAAETAKPAAITTEAL
                     FWVLLLVAIILMYVIYSMSEMIIWGVKRKMEQRKGGAGLLLLLAGTLLGSSSASAQDA
                     AATVVAAPAYNFWTDPYMPFYLLILVEVVVIAWMALVLFNMMRPEKAVEPEVQRVSVL
                     TAIWNKINPTLPVEREDEIKLDDHEYDGIVELGNKMPPWLQFIFYVTILFAVIYAPYY
                     LLGYGKTMDEKYQDELAEAELAKLERQKAGMNTVDENTVTLMQTSDVLSAGKETFLQF
                     CAACHGNYGEGGVGPNLTDDYWLHGGSINNVFTTIKYGVPEKGMASWQDILVPKQISE
                     VANYIKSLYGTNPPNQKEPQGDLWTDQPADTAAPDSTVVPVPADTMAVAVK"
     gene            3109..4500
                     /gene="ccoG"
                     /locus_tag="PK742_10020"
     CDS             3109..4500
                     /gene="ccoG"
                     /locus_tag="PK742_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQU76967.1"
                     /translation="MNDSGSFRDRIYTADEEGHRKWVYATQPFGRFYNYRTILTVLYV
                     VVFFALPFFKVDGHPLFLFDVPERKFILFGVIFWPQDFFVFAIAMLTFMVFIVFFTVI
                     YGRVFCGWVCPQTIFMEMIFRRIEYWIEGSAEKQRRMDSGPWNAEKILRKGSKHILFF
                     ILSFLISNTFLAYVIGMDDLVKIITEPVSQHLGGFIAIIGFTTVFYAVFAFAREMVCT
                     FACPYGRLQGVLLDRNSVVVAYDYQRGEPRGKKKRGEEQSTGDCIDCGLCVRVCPTGI
                     DIRNGTQLECINCTACIDACDSIMEKIDKPKGLIRYASENSIAENRPHRLTARMIGYS
                     VVLVLLIGALSVMLMTREEVDAQITRAQGQLYQQPDSTHYSNLYNIKLLNKTVDGFPV
                     ELRLEDVPGTINLVTHQELTVPPESYAESTFFIVLDEKDISKMKMDIKVGVYAHDKKV
                     ETVKTTFFGPMSN"
     gene            4507..>4809
                     /locus_tag="PK742_10025"
     CDS             4507..>4809
                     /locus_tag="PK742_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQU76968.1"
                     /translation="MNWGTGIVISFVVFIAMIFTFIFLGGRTKSDLVADDYYAQELAY
                     ESVIEATRNTLPFADSIYIRKNADGNILLTMPAHRADAVNGHIYFFRPADADLDRTM"
BASE COUNT         1233 a         1170 c         1178 g         1228 t
ORIGIN      
        1 gggtggtgtt cggtatcaat atgtttggta ccattatgaa gagaagagaa cgccatattt
       61 atgtggctat ctggttctac atagctacct tcctcacagt ggccatgctc catatcgtca
      121 actctattgc attgccagtg aatttcctga agagctatcc ggtatatgca ggtgtgcagg
      181 atgcattggt gcagtggtgg tacggacata atgcagtggc attcttcctc accaccccgt
      241 atctcggcct gatgtattac ttcctgccga aggctgccaa cagaccggta tattcttatc
      301 aattatccat cgtccacttc tgggcgctga tcttcctcta tatatgggca gggcctcacc
      361 acctgatcta tacgtcctta cctgactggg cacaaacgct tggtgtggtt ttcagcttta
      421 tgctgatcgc tccgtcctgg ggtggtatgg tgaacggact actcaccctg cgtggtgcct
      481 gggataaggt tcgcgatgat gtggtgctga agttcatggt agtggcagta tctgcttatg
      541 gtatgtccac ctttgaaggc cctatgctgt ctcttcgcaa catcaatgcg tgggcacatt
      601 tcacagactg gatcgttgcc cacgtacacg tgggtgcact cggctggaac ggcttcctga
      661 cattcggtat cctgtactgg cttgttcccc gcatgtggaa gaccaatctg tacagtaaat
      721 cactggccaa tacccatttc tggataggta cgctgggtat catcttctat gcagtgccca
      781 tgtactgggc cggctggatg gaaggctcca tgtggaagca gttcaatgat gatggcatgc
      841 tgcagtatcc cactttcata gagaccacac agtatgtgaa acctttctat gccatgcgtt
      901 ccattggtgg ggtattctac ctgaccgggg tgttcatcat ggcctataac ctcgtcaaga
      961 ccgcacgtca gggaagcttc atggcaagtg aagaagctga agctgctccg atggaaaaag
     1021 ttgtgaaggt ctataagggt gaattctggc atcgtgccct ggaacgtcgc ccgatacaat
     1081 tcggaatgct ggcccttgtg gctgtactga taggtggtat cgtcgagctg gtacctacct
     1141 tcctggtcaa gtcaaatgta cccaccatcg cttccgtgca accctataca ccattggaat
     1201 tgcaggggcg tgatatctat gtcagggagg ggtgttatac ctgtcactca caaatggtca
     1261 gacccttccg caatgaagtg gttcgctata aaggagaata ctcaaaagca ggtgagttcg
     1321 tctatgacca ccctttccaa tggggtagta agcgcaccgg tcctgacctg cacagagaag
     1381 gcggtaaaca gaccaatgca tggcattact ggcacatgta tgagcccacc agtatgacct
     1441 ccggctctat tatgccttct tatgcctggc tgcatgaacg cacacttgac atcagtacca
     1501 cacctgccaa gatcagggtg atgcagaacc ttggagtgcc ttatcctgaa ggatatgatg
     1561 ctattgccaa tgacgatctc aagttccagg cagaacagat cgccgcgaaa ctggcccagg
     1621 aagacagccg gatcaagacc tctgcagaca aagagatcat agcattgatc gcttacctgc
     1681 agcgactcgg cacagacatt aaagcagaac ccacagctca ggctgcaaac tgatccatat
     1741 gagtttcata cattatctcg aaggtatttc cggcataggc atctatccga tgttctccct
     1801 gctcgtgttc ttcatctttt ttgtagtact gctcttcctg gtttggaaga tgaacagatc
     1861 taccattgaa gaactgaaac agatacccct gcaggaccag gagctggcct ctgatcatac
     1921 aaccactcaa aatcactgat atgaaaaccg gaaaccgaaa tatcctccgt ttgctttcac
     1981 tggcaggctt gacggctttc agtacttccc tttttgcaca gaatgctgca gctgaaacag
     2041 caaaacctgc ggccatcact acagaagctt tgttctgggt gctattgctg gttgccatca
     2101 tactgatgta tgtcatctac tccatgagtg aaatgatcat atggggtgtg aagcgtaaaa
     2161 tggaacaacg caaaggcgga gccggcctgc tgctgttgct ggcaggtaca ttgcttggat
     2221 cttcctcagc cagcgctcag gatgctgcgg caaccgtggt ggcagcaccg gcatataatt
     2281 tctggaccga tccgtacatg cccttctatc tgctgatcct ggtagaagta gtggtcatcg
     2341 cctggatggc actggtgctg ttcaatatga tgcgccctga aaaggccgtt gagcctgaag
     2401 tgcagcgtgt ctctgtactg acagccatct ggaacaagat caatcctacc cttccggtgg
     2461 aacgggaaga tgagatcaaa ctggatgatc atgaatatga cgggatcgtg gaacttggca
     2521 acaagatgcc accctggctg cagttcattt tctatgtcac catcctgttc gcggtcatct
     2581 atgcaccata ctacctgctg ggatatggca agaccatgga tgaaaaatat caggatgaac
     2641 tggccgaagc agaactggct aaactggaac gacagaaggc aggcatgaac acagtggacg
     2701 agaacaccgt gaccctcatg caaacctcag acgtacttag tgccgggaaa gagaccttcc
     2761 tgcaattttg tgctgcctgc catggcaatt acggtgaagg cggtgtaggc ccaaacctga
     2821 ccgacgatta ctggctccat ggcggatcca tcaacaatgt attcactacg atcaaatacg
     2881 gagttcctga aaaaggcatg gcttcctggc aggatatcct ggtacccaaa cagatcagcg
     2941 aagttgctaa ctatatcaaa tcactctacg gcaccaatcc gccaaaccag aaagaaccgc
     3001 aaggcgattt atggacggat caacccgcag atacagcagc acctgattca actgttgtac
     3061 ctgtaccggc tgatacgatg gcggtagccg tcaaatgacg tacgcatcat gaatgattca
     3121 ggctcattca gagaccgtat ttatacggcc gacgaagaag gacaccggaa atgggtatat
     3181 gctacccaac catttggcag gttctacaac taccgcacca tacttacagt attgtatgta
     3241 gtagtgttct ttgccctgcc attctttaaa gtagatggac atcctttgtt cctgtttgat
     3301 gtccccgaac ggaaattcat tctgttcggg gtcatcttct ggcctcagga tttttttgtt
     3361 ttcgctatcg ccatgctcac cttcatggtg ttcattgtat tctttaccgt gatctatgga
     3421 agggtcttct gcgggtgggt ttgtccgcaa accattttca tggagatgat attccggcgc
     3481 atagaatact ggatagaagg ctcggcagaa aaacaaagga ggatggacag cgggccatgg
     3541 aatgcagaga agatcctgcg taaaggaagt aagcatatcc tgtttttcat cttgtctttt
     3601 ctcatttcca atacctttct ggcgtatgtg atcggcatgg atgatctggt caagatcatt
     3661 accgaaccgg tcagtcagca ccttggtggt ttcatcgcca tcatcggttt cacaaccgta
     3721 ttttatgccg tgttcgcatt tgcccgtgaa atggtgtgca ccttcgcctg tccctatgga
     3781 agactacagg gcgtactgct tgacagaaat tctgtggtgg ttgcctatga ctatcagcgg
     3841 ggagaaccca gggggaaaaa gaagagaggt gaagaacaat ctaccggcga ctgtatcgac
     3901 tgcgggttgt gtgtccgtgt gtgccccacc ggtatagata tccgcaacgg tacccagctg
     3961 gagtgtatca actgtacagc ctgtattgat gcctgcgatt ccatcatgga aaagatcgat
     4021 aaacccaagg ggcttattcg atatgcatct gaaaacagca ttgcggagaa cagaccacac
     4081 aggcttacag ccagaatgat cggctacagt gtagtactgg tattgcttat cggggcactc
     4141 agtgtcatgc tgatgaccag agaagaagtg gatgcacaga tcacacgtgc acagggacag
     4201 ctctatcagc aacccgacag cacccactac agcaatcttt acaacatcaa actgctcaac
     4261 aaaacggttg atggtttccc tgtagaactg aggctggaag atgtacccgg caccatcaat
     4321 ctggtaaccc atcaggagct gacagtacca cccgaaagct atgctgaaag taccttcttc
     4381 atcgtactgg atgagaaaga tatcagcaaa atgaagatgg atatcaaagt gggtgtgtat
     4441 gcccacgata aaaaagtaga aacggtcaaa acgacctttt tcggaccaat gtcaaactaa
     4501 tagcatatga actggggaac aggcatcgtc atttcatttg tggtattcat tgccatgatc
     4561 tttactttca ttttcctggg aggaagaacc aaaagtgatc tggttgccga cgattattat
     4621 gcccaggaac tggcatatga atcggtcatt gaagcaacac gcaataccct gccttttgcc
     4681 gatagcatct acatcagaaa aaatgcagat ggtaatattt tgcttaccat gcctgctcac
     4741 cgggctgatg cggtaaacgg tcatatatac ttcttccgcc cggcagatgc agatcttgat
     4801 cgcaccatg
//
LOCUS       DAOMAX010000206         4792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3066_length_4792_cov_3.696010, whole genome shotgun sequence.
ACCESSION   DAOMAX010000206 DAOMAX010000000
VERSION     DAOMAX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_4792_cov_3.696010"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="lepA"
                     /locus_tag="PK742_10030"
     CDS             complement(<1..1067)
                     /gene="lepA"
                     /locus_tag="PK742_10030"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQU76969.1"
                     /translation="MQHIRNFCIIAHIDHGKSTLADRLLETTSTISERDRQDQVLDDM
                     DIERERGITIKSHAIQMDYVQDGTRYTFNLIDTPGHVDFSYEVSRSIAACEGALLLVD
                     CTQGIQAQTISNLYLAIEHDLEIIPIVNKIDMDGAMIEETKDQIVDLIGCDPGSIIEA
                     SGRTGIGVDAILRAIVERIPAPKGDPDSPLQALIFDSVFNSYRGVVAYFRIYNGTLKK
                     GDKVKFIHTKKEYEADEVGILRMEYEPRAEVKAGDVGYIITGIKQAKEIKVGDTITHT
                     SMSSASVQGFQDVKPMVFAGIYPIDNDDYEDLRESLEKLQLNDASLVYEPESSVALGF
                     GFRCGFLGMLHLEIVQERLER"
     gene            1218..1718
                     /locus_tag="PK742_10035"
     CDS             1218..1718
                     /locus_tag="PK742_10035"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQU76970.1"
                     /translation="MFSRILAFLIDTILMGAIVVGLLFVTMGKTPASLKASGDEAPPS
                     LMDKYQFFAYFEPIAMDPNRDIYIKRFIQNYPAQGGVAFILIPWLWFALMESMFGGSI
                     GKLATGIRVRRKDYGKAGLGVTTVRLIGKAISTLIVGLGYLLAFFDKKNQALHDKIAN
                     TLVVKK"
     gene            complement(1720..2313)
                     /locus_tag="PK742_10040"
     CDS             complement(1720..2313)
                     /locus_tag="PK742_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76971.1"
                     /translation="MRIFVLCTGRTGSQSFIEACRFIDNFSCGHESRRSLLGSDRLDY
                     PPNHIESDNRLSWMLGPLADRFPDDVFYVHLIRDEEATARSFNRRWNNPWSIIHAWAE
                     AVLFRNIRDLTDDEKLQIARDYVRTTNLNIASFLRNKPHLTVRLEHIQEDFRLFWHHI
                     QATGNLEEAIGSLTTPHNTGDENELQKSRSFSIFRKR"
     gene            complement(2337..4421)
                     /locus_tag="PK742_10045"
     CDS             complement(2337..4421)
                     /locus_tag="PK742_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76972.1"
                     /translation="MRRSAFTGILLLSCTCMLQAQNPVNEPGPETDLIEYLVEDQEES
                     EFGFDTFLETLEYLQQNPLRINKTDYDTWRQTGLLTELQIRQLLRHIEQTGPLHNIYE
                     LQTIASFSTEDILRLQPYVIWDAYDGTTLPYLQQLVQGRYALFVRSTRVLQEQRGYQT
                     EDSTLSSPYLGDPNRLYSRFSYNYKNALRYGFTVEKDPGEPFSGVPGFDYWSAHYFQK
                     GNGWIKALAIGDYELRIGQGLIVWSGFGMGKSIYPVNIKRTRPVLAPYTSVDENRFFR
                     GAAATVGKGPLELTVFGSYNQVDANLIASQDTSLSDPGDEGTVTISSLQLTGLHRTES
                     EIADKDAVGFMATGGNLAWRKGPLSVSAAGILYHFDAELVEDRSPYELYDFEGSQLIN
                     ASLSYNYVWRNWLLFGETAISDNGKAGTLNGLILPVDKHVDLALVYRYFDPGFQTFFS
                     QSFSENTYPRNEEGWYTGIEITPVKAWRFNAYADIYKKPWLSFTADAPAYGADYLATV
                     YYKPKRTLETYVRLRYEASDRNANAAIQGDLPHDIITTERKLRVRWNISYQLTREVTL
                     RNRIEWSQYGQDGGTTENGYLLYQDVVYKPWELPFSLACRYTLFSTDTYNTRIYAYEN
                     DLLYAYSIANIYGEGQRVYAMVQYSPTGWLDTWIKYGQTVYTDRDTIGSGNEAIEGNR
                     RSELRLQVRFKW"
     gene            complement(4418..>4792)
                     /locus_tag="PK742_10050"
     CDS             complement(4418..>4792)
                     /locus_tag="PK742_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76973.1"
                     /translation="ALLYKPSPELSIGARAYNPFMASAGEDLPGLEISSLLAVGLTYQ
                     PAKQFQLSAEGEQTIGYDFNLKAGAEYRPVEILAIRGGWMSRSSVLTAGFGIRWQQLF
                     IDLGAQFHPQLGTSPAIGIHLE"
BASE COUNT         1154 a         1164 c         1173 g         1301 t
ORIGIN      
        1 tcacgctcca gtctttcctg tacgatctcc aggtgcagca ttcccagaaa gccgcagcgg
       61 aatccaaaac ccagtgcaac ggaactttcg ggctcataca caagggaggc atcattgagc
      121 tgcagttttt ccagactttc acgaaggtct tcataatcat cgttgtcgat cggatagata
      181 ccagcgaaga ccatgggttt cacatcctga aaaccctgta cggaggcact ggacatagaa
      241 gtgtgcgtga tggtatcgcc taccttgatc tcttttgcct gtttgattcc ggttatgata
      301 tagcccacat cgccggcttt tacctcagct ctgggttcat attccatccg gagaataccc
      361 acttcatctg cttcatactc ttttttggta tgaatgaatt ttactttgtc gccttttttc
      421 aaggtgccgt tgtagatccg gaagtaagcc acaacccctc tgtaggagtt gaacacagag
      481 tcaaagatca gcgcttgcag aggtgaatcc ggatctcctt tgggagcggg aattcgttct
      541 acaatggcac gcagaatagc atccacacct atacctgttc tgccgctggc ttcaatgatg
      601 ctgccggggt cacagccaat caggtcaacg atctggtctt tggtctcttc tatcatggcg
      661 ccatccatat cgatcttgtt cacgatcggg atgatctcca ggtcgtgctc aatggccaga
      721 tacaggttgg aaatggtctg tgcctggata ccctgcgtac agtcaaccag caacagagca
      781 ccctcacagg ctgcaatgga gcgggatacc tcatagctga agtcaacatg ccccggagta
      841 tcaatcaggt taaaagtgta gcgggtacca tcctgcacat agtccatctg aatggcgtgg
      901 cttttgatgg taatgccgcg ttcacgttcg atgtccatgt cgtccagtac ctggtcttgt
      961 ctgtcacgtt cagaaatggt actggtagtt tccagtaagc gatcggccag ggtacttttt
     1021 ccatggtcaa tgtgggcaat gatgcaaaaa ttacggatgt gttgcatagt tgggcgtgac
     1081 agcgggcaaa attacggaaa tagccgcagg agctgaaata acgaatggta ttccggccgg
     1141 cttgttctat aaaatggtta acattgcact agcaattgaa acctttttct atgacgcatt
     1201 catccaaacc agcacctatg ttcagccgta tcctggcatt tctgatcgat accatattaa
     1261 tgggtgccat cgtagtgggt ctgttgttcg taacaatggg aaagaccccg gcttcactga
     1321 aggcctcagg tgatgaggca cctcccagtc tgatggataa gtatcagttt ttcgcctatt
     1381 tcgagccgat agccatggat ccgaacaggg atatttatat caaacgcttc atacagaact
     1441 atccggctca gggcggtgta gcctttatac tcattccctg gttgtggttc gctctgatgg
     1501 agtctatgtt tggtggttca attggtaagc tggctacagg tatccgggtg cgcagaaaag
     1561 attacggcaa ggccggatta ggtgttacta ctgtcaggct gataggtaaa gccatctcca
     1621 ccctgatagt tggtctgggc tatctgctgg cctttttcga caaaaagaac caggctttgc
     1681 acgacaagat cgccaataca ctggtggtta aaaagtgatc tatcgctttc tgaatatgct
     1741 gaacgatcgg cttttttgca gttcgttctc atcgcctgta ttgtgcggag tggtcagtga
     1801 cccgatggct tcttccagat ttccggtggc ttgaatgtga tgccagaaca aacggaagtc
     1861 ttcctgtata tgttccagtc tgacggtcag gtgcggtttg ttccggagga aggatgcaat
     1921 gttcaggttg gtggtcctta cataatcgcg ggctatttgc agtttttcat cgtcagtaag
     1981 gtcgcggata ttcctgaaca ataccgcttc agcccaggcg tgtatgatac tccaggggtt
     2041 gttccatcgt ctgttgaagc ttctggcagt tgcttcctca tctctgatca ggtgaacata
     2101 aaacacatcg tccggaaagc gatcagccaa aggaccaagc atccagctta acctgttgtc
     2161 tgattctata tggtttggcg gatagtccag tctgtctgaa cccagcagtg aacgtctgct
     2221 ctcatgacca cagctgaagt tatctatgaa cctgcaggct tcaatgaatg attgcgatcc
     2281 ggttctgccg gtgcaaagca caaagattcg catggatgca aagtacgcaa aaccggctac
     2341 catttaaaac gaacctgcag gcgaagttct gaccttcggt ttccttcaat ggcttcattt
     2401 ccgctgccaa tagtatcgcg gtctgtatag acggtctgac catatttgat ccaggtatcg
     2461 agccagcctg tagggctgta ttgtaccatg gcataaaccc gttgcccttc accataaata
     2521 ttggcaatac tgtaggcata taacagatcg ttttcatagg catagatacg ggtgttatag
     2581 gtatctgtgc tgaaaagggt gtagcggcaa gccagagaaa aaggcagctc ccatggttta
     2641 tacacaacat cctgatacag gaggtatccg ttttcggtgg tgccaccatc ctggccgtac
     2701 tgactccatt ctatgcggtt gcgaagtgtc acttcacggg taagctggta gctgatattc
     2761 cagcgaactc tgagttttct ttcggtcgtg atgatatcat gtggcagatc tccctgaata
     2821 gccgcattgg catttctgtc tgatgcttca taccgaagcc tgacataggt ttccagcgtg
     2881 cgcttaggtt tgtaatatac agtagccagg taatctgctc cataagctgg cgcatcggcc
     2941 gtaaaggaaa gccagggttt tttatagatg tctgcatagg cattgaaccg ccaggccttt
     3001 accggtgtga tctcaattcc tgtgtaccat ccttcttcgt ttcgagggta ggtgttttct
     3061 gaaaacgact ggctgaaaaa tgtctggaac ccggggtcga aataacggta cacaagggcc
     3121 aggtcaacat gcttgtcaac cggcagtatc agtccgttca aggtgcctgc cttgccatta
     3181 tcacttatgg cagtttcgcc aaagagcagc cagtttcgcc acacataatt ataggaaaga
     3241 gaagcattga tgagttgaga tccttcaaag tcatacaact cataggggct tctgtcttcc
     3301 accagttcag catcaaaatg atagaggatg ccggctgcag aaactgacaa agggcctttt
     3361 cgccacgcca gatttccacc tgttgccata aaaccaacgg catctttatc tgcaatttcg
     3421 ctttcggtcc ggtgtaaccc ggtaagctgc agagatgaaa tggtcactgt tccttcatct
     3481 cccggatcag aaagactggt atcctgcgag gcgatcaggt tggcatctac ctggttatag
     3541 ctgccaaaca cggtcagctc cagggggcct tttccaactg tggcagcggc gccccgaaag
     3601 aaccgatttt cgtcaacaga agtataaggt gccagcaccg gccttgttct tttaatattg
     3661 acaggataga tgcttttacc cattccgaaa ccactccaga cgatcagtcc ctgtccgata
     3721 cgcaattcat aatcgccaat ggccaatgcc tttatccagc cgtttccttt ttgaaaatag
     3781 tgtgcactcc agtaatcaaa gccaggtacg ccgctgaaag gttcacccgg atctttttct
     3841 actgtgaacc catatctaag tgcgttctta taattataag aaaatctgct atacaatctg
     3901 ttgggatcac ccaggtacgg actgctcagg gttgagtctt ctgtctgata gccccgctgc
     3961 tcctgtaata ccctcgtgct tctgacaaac agggcatagc gtccctgtac cagttgttgc
     4021 aggtaaggca gtgtggtgcc gtcatatgca tcccagatca catacggttg caatcgcaga
     4081 atatcttctg tactgaatga ggctatggtc tgtagctcat agatattatg cagggggccg
     4141 gtttgctcaa tatgccgaag cagttgcctg atctgcagct cggtcagcaa accggtttgc
     4201 cgccaggtgt cataatcggt cttattgatg cgtagtgggt tctgttgcag atattccagt
     4261 gtttccagga aggtgtcaaa gccaaattca ctttcttcct gatcttctac caggtattca
     4321 atgaggtcag tttctggtcc gggttcattg acaggatttt gtgcctgcag catacatgtg
     4381 caggataaca gcaggatacc ggtgaaagca gatcttctca ttccaggtga atacctatgg
     4441 caggtgaggt gccgagctgc ggatggaatt gcgctccgag gtcaataaag agttgctgcc
     4501 acctgatgcc aaatccggct gtcaatacgg aagaccggct catccagcca ccgcgaattg
     4561 ccaggatctc aacaggcctg tactcagctc cggctttcag attgaaatca tagccgatgg
     4621 tctgctcgcc ctctgctgac aactgaaact gcttggccgg ctggtaggtc aaacccactg
     4681 ccagcagtga agatatttcc agcccgggaa ggtcttctcc ggctgaagcc ataaagggat
     4741 tgtatgccct ggctcctata ctcaactcag gcgaaggttt atacagcaaa gc
//
LOCUS       DAOMAX010000207         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3074_length_4786_cov_4.380681, whole genome shotgun sequence.
ACCESSION   DAOMAX010000207 DAOMAX010000000
VERSION     DAOMAX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_4786_cov_4.380681"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK742_10055"
     CDS             <1..768
                     /locus_tag="PK742_10055"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HQU76974.1"
                     /translation="DSWEPTPPDNADAVEPYWGRMRPFVLDSASQCQPIDPPAYSEDP
                     SSRFYLDAMEVFDTKTTLTADQEATVFFWDDNPFVTVHTGHLVYAKKKVSPAGHWMGI
                     TEIAINQSGADLDKAIEAYTMVSIGLADAFISCWEEKYRSNLIRPVTYINRVIDPNWE
                     PFLQTPPFPEYTSGHSVISASASMILTELFGDGFAYTDDVLEKYGQPPRSYKSFLDAA
                     NEAAYSRLYGGIHYRPAIEVGVQQGRKVASIIIAELL"
     gene            895..1623
                     /locus_tag="PK742_10060"
     CDS             895..1623
                     /locus_tag="PK742_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76975.1"
                     /translation="MKKFVLFSFALVAVGALSAQNFKVTYNANLGEGTMGAGAAAKAY
                     WHAGAGELGPWENTVGNWGMDDGVGEMTESADDIWELTYDASTYWADAGYGGTVPPPH
                     IGMVFRNEDGTLEGKNYGLSDIFAVWNDGTMSYDLDCDCVTIEEVVPQNINNTTSTVG
                     SLNVAPMPFGNTANFNFTLETANNVVISVYNMMGAEVAQVFNGNLAAGVHTVAFDASD
                     LISGNYIFRMQVGSEVAGGSIVKF"
     gene            1789..4572
                     /locus_tag="PK742_10065"
     CDS             1789..4572
                     /locus_tag="PK742_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU76976.1"
                     /translation="MKKIFFAIVVITTTLTRAQVVSTDPAFPVQDGTVTIIFDATQGN
                     GALAGVAPPIFAHTGVVTNESATETSWLLVQGNWGTYDENVLMTEIGDDLYSITYNIN
                     DYYSVPAGDTVFRMGFVFRNTDGSIVGRNADGSDIFVEVYQPGLNVAILTPDIEPYII
                     DADEEVNISIASLDATVTELYIDDVLTASTATDLLEYTWSTSDAGLHTIIAVAGDGSD
                     EVSDTTHIYVKGPVTIADLPAGAEPGINYVDDTTVTLVLYAPFKDYVFAIGDHSDWQV
                     NDAVYMNRTPDGNTYWVTLTGLTPGEEYAYQYLVDGTLRVGDPLSEKVLDPWNDSFIS
                     SSNYPDLKPYPTGKTMGIVSVFQTAQAPYVWAYPDFTPPAKENLFVYELLVRDFLADR
                     SYNSLIDSLEYLDRMGVTAIELMPIMEYEGNDSWGYNPSYFTALDKYYGTRETFKAFV
                     DSCHARGIAVILDIAMNHAFGQSPLVQMWWDGSAPSAESPYFNQIPTHDYNVGYDFNH
                     EAPATIDYRTRVFSYWLNEYNIDGYRFDLSKGFTQNNTLGDVGAWGAYDAGRIATWKS
                     IADTLWAQHPDAILILEHFADNTEEKELANYGFMLWGNMNYNYNEATMGWGNGSGNSS
                     LYWASWKNRGFDDPHNVVYAESHDEERLMYRNISFGNASNPDHNCKDLYTALARMEQV
                     PVFLFGIPGPKMIWQFGELGYDYSIDYGCRVCAKPVKWNYLNESARYRLYQVYGAMGN
                     LKKDYPTFATEDFNLSATGPLKRINLDNDEMNATILGNFEVNTNSIQPAFQHTGWWYE
                     YFSGDSINVTDVFAEISMAPGAYRLYTDVRLTTPEIIISVDDAEVVADNLAVYPNPSD
                     GLFQFSWTTYSATDVQIEIFDINGRMIWQQTGSYAAGYQQQTIDLSAQASGIYFARVS
                     GEGFGEVMRLVRK"
BASE COUNT         1240 a         1215 c         1200 g         1131 t
ORIGIN      
        1 gacagctggg agcccacccc tcctgataat gcagatgccg tagaacctta ctggggacgg
       61 atgcggcctt ttgtactgga cagcgccagc cagtgccaac caattgaccc accggcatac
      121 agtgaagatc catcctcccg gttttatctg gatgcgatgg aggtctttga taccaaaact
      181 acattgacag ctgatcagga ggccaccgtt ttcttctggg atgataatcc gtttgtaacc
      241 gtacataccg gccatcttgt atatgccaag aaaaaagtat cgccggcagg tcactggatg
      301 ggtatcacag agatcgccat caaccagagt ggcgcagacc tggacaaagc catagaagcc
      361 tacacgatgg tctccattgg tctggcagat gccttcatct cctgttggga agagaaatac
      421 cgcagcaacc tcatcaggcc ggtcacctat atcaaccggg tgatcgaccc aaactgggag
      481 ccatttctgc aaaccccgcc gtttccggaa tacaccagcg ggcacagcgt catctctgcc
      541 agcgccagca tgatcctgac cgaattattc ggcgatggtt ttgcctatac cgatgatgtg
      601 ctggaaaaat acggccagcc gccccgcagt tataagtctt ttctcgatgc cgccaatgaa
      661 gctgcttaca gcagactgta cggtggcatt cactacagac cggctattga agtaggggtg
      721 caacagggca gaaaggtggc ttccattata attgccgaat tgttatagtg tgcagcatat
      781 tgttcaacat gccgcaatca ggttaatttt tcctgtaaga attgttacgt atttgcggaa
      841 ttcctttaat tttagtccga ttttttagat ctaaaaaaaa caattgaacg tatcatgaaa
      901 aaatttgtac tcttttcttt cgccctcgtg gcggtgggtg ccctgagtgc tcagaatttc
      961 aaagtaactt acaacgccaa cctcggtgaa ggtaccatgg gtgccggtgc tgcagcgaag
     1021 gcatactggc acgctggtgc cggtgagctc ggtccctggg aaaacactgt tggtaactgg
     1081 ggtatggatg acggtgtagg tgaaatgacc gagtctgcag acgacatctg ggaactgacc
     1141 tatgatgcat ctacttactg ggctgatgct ggatacggcg gaacagttcc tcctccccac
     1201 atcggtatgg tcttccgaaa cgaagacgga accctggaag gtaagaacta tggtctttcc
     1261 gacatctttg ccgtatggaa cgatggtact atgtcctatg atctcgattg tgactgtgtg
     1321 actattgaag aagtggttcc gcagaacatc aacaacacca cttctaccgt aggtagcctg
     1381 aacgtggctc cgatgccttt cggcaacact gccaacttca acttcaccct ggagaccgct
     1441 aacaacgtag tgatctctgt ctataacatg atgggtgctg aagtagctca ggtattcaac
     1501 ggtaacctgg cagccggtgt gcacacagta gcattcgacg ccagcgacct gatcagcggt
     1561 aactatatct tccgcatgca ggttggtagc gaagttgccg gtggcagcat cgttaagttc
     1621 tgatcgaacg aaaccttata gaaatgaggc cgtctctgca gggacggcct ctttcttttt
     1681 gccacagtct gacaaacgaa gtacagccca aaaccctccc gtcgtatatt tgttatacag
     1741 aataaagcgg ttgtttcctt cagaacaacc tatagcaaag gcatacacat gaaaaagata
     1801 ttcttcgcga tcgttgttat aacaaccact ttgacccggg cccaggtggt cagtaccgac
     1861 cccgcctttc cggtgcagga tggtacggtc accatcattt ttgatgccac ccagggcaac
     1921 ggggcactgg ctggtgtggc accccccata ttcgcacaca cgggtgtggt gaccaatgaa
     1981 agtgccacag aaacttcctg gctgctggtg cagggaaact ggggtaccta cgatgagaat
     2041 gtactgatga ccgagatcgg agacgacctg tacagcatca cctataacat caacgattat
     2101 tacagtgtgc ccgccggcga tacggtcttc agaatgggtt ttgttttccg caataccgac
     2161 ggttccatcg tagggcggaa tgcagacggc tctgatattt ttgtcgaagt atatcagccc
     2221 ggcctgaatg tggccatcct tacacccgat atcgaaccct atatcatcga cgcagatgaa
     2281 gaagtgaaca tcagcattgc atcactggat gctaccgtca ccgaactgta tatcgatgat
     2341 gtgctgactg ccagcacagc aaccgatctg ctggaatata cctggtcaac cagcgatgcc
     2401 ggactccata ccatcatcgc tgttgccggt gatggcagcg acgaagtgag cgacaccacc
     2461 catatctatg tgaaagggcc tgtgaccatt gccgatctgc cagccggtgc cgaaccgggt
     2521 atcaactacg tggatgatac cacagtgacc ctggtattgt atgcaccttt caaagactat
     2581 gtgtttgcca tcggcgatca cagcgactgg caagtgaatg atgcggtata tatgaaccgc
     2641 acaccggacg ggaatactta ctgggtcaca cttaccggac tgaccccggg cgaagaatat
     2701 gcctatcagt acctggtgga cggtacactc cgggtgggtg atccgttgtc ggaaaaagta
     2761 ctcgatccct ggaacgattc ttttatcagc agcagcaatt atcccgatct gaaaccttat
     2821 cccaccggaa agaccatggg tatcgtgtct gttttccaaa ccgcacaagc accttatgtc
     2881 tgggcttatc cggatttcac cccaccggca aaagagaatt tgttcgtata tgaattgctg
     2941 gtgcgtgatt tcctggccga ccggtcctat aactcactca ttgattcact ggaatacctg
     3001 gatcgcatgg gcgtgaccgc tattgaactg atgcccatca tggaatacga aggcaacgac
     3061 agctggggtt ataacccaag ttattttacg gcactggata aatactacgg tacccgtgaa
     3121 accttcaagg cctttgtgga cagttgccac gcacgaggta ttgccgtcat cctggacatt
     3181 gccatgaacc acgctttcgg ccagagtccg ctggtacaga tgtggtggga tggctctgcg
     3241 ccttcagcag aatcgcccta tttcaaccag atacccactc acgattataa tgtgggttat
     3301 gatttcaacc acgaagcacc tgctacgata gactaccgta cgagggtgtt ctcttactgg
     3361 ctgaatgaat acaacattga cggataccgc tttgatctgt cgaaaggatt tactcaaaac
     3421 aatacactgg gagatgtagg cgcctggggt gcctatgatg cagggcggat cgctacatgg
     3481 aaatccattg cagatacgct gtgggctcag caccctgatg ctattctcat cctggaacat
     3541 tttgcagaca atactgaaga aaaagaactg gccaactacg gcttcatgct gtggggcaac
     3601 atgaactaca attacaacga agccaccatg ggctggggta acggcagcgg caattcaagc
     3661 ctgtactggg cttcgtggaa gaatagaggg ttcgatgatc cgcataatgt ggtctatgca
     3721 gagagccatg acgaagaacg gctcatgtac cggaacatca gctttggcaa tgccagcaac
     3781 cccgatcaca attgcaaaga cctttataca gcacttgcca gaatggaaca ggtaccggtg
     3841 ttcctgttcg gcatacccgg accaaaaatg atctggcagt tcggcgaact ggggtatgac
     3901 tacagtattg attacggttg ccgggtctgt gccaaaccgg tcaaatggaa ttacctgaat
     3961 gaatctgcac gatacagact atatcaggtg tatggtgcta tgggtaatct gaagaaggac
     4021 tacccaacgt tcgccacaga agatttcaat ctgtcagcaa ccggtccgct gaagcgcatc
     4081 aacctggaca acgatgagat gaacgccact attctgggca atttcgaagt gaacaccaac
     4141 agcattcaac ccgccttcca gcacaccggc tggtggtatg aatacttcag cggcgacagc
     4201 atcaatgtga ccgatgtgtt cgcagagatc agcatggcac cgggcgctta cagattatac
     4261 actgacgtac gactgaccac accggagatc atcatttcag tggatgatgc tgaggtagtg
     4321 gcagataacc tggcggtgta tcccaatccc tccgacggac tgtttcagtt cagctggacc
     4381 acctattctg caaccgatgt acagatagag atcttcgaca tcaacggcag gatgatctgg
     4441 cagcaaaccg gttcctatgc agcgggctat cagcagcaaa ccatagacct gagcgcacag
     4501 gcatcgggca tctactttgc ccgtgtgagc ggagagggtt tcggtgaggt gatgaggttg
     4561 gtgagaaagt gaatcccgcc agtggcggga agtgaatggt gagagtcctt atcaaaagga
     4621 tacactactg actgatcaca gctcaccact cactcatgat catctggaca atgtacaacc
     4681 gggctgtgtc taacttcatg acacagctcc ttatcttgcg ggcataagca ccgcgattgt
     4741 cgcacccact taaaaaacta ttgtcgcaga cagccatcta tggcct
//
LOCUS       DAOMAX010000208         4770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3088_length_4770_cov_4.188971, whole genome shotgun sequence.
ACCESSION   DAOMAX010000208 DAOMAX010000000
VERSION     DAOMAX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3088_length_4770_cov_4.188971"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK742_10070"
     CDS             complement(<1..233)
                     /locus_tag="PK742_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU76977.1"
                     /translation="MKQFRTFVYKEFLHIIRDPKSLLIIMALPIVQIIIFGFALTNEI
                     KNADIGILVTEPDEATRQLTERLDASRYFNLALV"
     gene            complement(235..972)
                     /locus_tag="PK742_10075"
     CDS             complement(235..972)
                     /locus_tag="PK742_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU76978.1"
                     /translation="MKPVITVRDLTRQFGTFTAVDHISFEVHPGEIFGFLGANGAGKT
                     TAMKMLIGILRPSSGEASVAGFDVYREAEHIKQHIGYMSQRFSLYEDLTVMENIRFYA
                     GIYGLPITSIRKEAKALLDRLELTHEANTLVKALPLGWKQKLAFSLAIIHKPAIVFLD
                     EPTGGVDPITRRRFWEMITETARTGMTVFVTTHYMDEAEYCDRVSIMVDGRIEALDHP
                     KKLREQYGAQSMDEVFRILARGAVRSE"
     gene            complement(976..1887)
                     /locus_tag="PK742_10080"
     CDS             complement(976..1887)
                     /locus_tag="PK742_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU76979.1"
                     /translation="MKSITVHNIIKTFPGKPPVQALQGVNLDIEKGELFGLIGPDGAG
                     KSTLFRLIASLQLPDSGSITMEGLDVLKDYMKVRKLIGYMPGKFSLYQDLSVEENIRF
                     FATLFNTTLEDNYHLVKDIYIQLEPFKKRRAGKLSGGMKQKLALCCALIHKPEVLLLD
                     EPTTGVDPVSRKEFWEMLHRLKALGITVLVSTPYMDEAMQCDRVALMQHGTLLQVDTP
                     DNIIASYNKPIYAVTGNNSPEVLHALREWEGSYSCFAFGEALHLTLQSEQYSVESVRK
                     FLDEKNINGVEMEAITPGIEDCFMAYL"
     gene            complement(1887..2771)
                     /locus_tag="PK742_10085"
     CDS             complement(1887..2771)
                     /locus_tag="PK742_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQU76980.1"
                     /translation="MKKYLILFVPFFIACNGEEKAFDATGTFEAREVIVSAEQTGKLI
                     DYAVDEGQMLEANARVAEIDSAQLVLKKAQLEAQIKAVLSRQPDVQTQLAALQEQIKT
                     ANFELNRLENLVKGGAATQKQLDDANAQIDVLEKQLAAQRSALQTTTSSIQSEVAPLS
                     LQIEQVNDLLNKCHVVNPINGTVLTSYVEAGEIAVAGKPLYRIADLSHMELRAYVTGD
                     QLATLSIGQKVTVSTDNGSGGFNQQEGSIIWISEEAEFTPKTIQTKDERANLVYAIRI
                     SVPNDGKLKIGMYGEVSW"
     gene            complement(2776..4044)
                     /locus_tag="PK742_10090"
     CDS             complement(2776..4044)
                     /locus_tag="PK742_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQU76981.1"
                     /translation="MKKHAIWNFLLIIPMLASAQDPALLDLQKCYRAAEAKFSLDEQQ
                     TLISHKNEAQQKVAGSYWQPQVNLNAQATYQSDVVGIPLESPLFEIPTLSKDQYRATI
                     DVTQTLYDGGLVHQKKTMSDLQAAVESASVSSQFYSIYDQIDQLFTGILLARQQQLLI
                     YSTKKDLENRKGQLETAYKNGTATAAQVKTLEAEILRLNQRMDDARSMEQGAIRALGQ
                     LTGLAIPDDTQLIPPGNAMPGSAELHRPELDLIDKQIELAQAGEGFSDALVLPRVSAF
                     VQGGYGRPGLNYLVDEFDTFYMLGVRLNYPLWSGGKNRNEAETWRIQQEQLALQKQNM
                     SSSIQVLSVRQHEEVNRLQGVLSTDDQIIALREEVLSSSKVAMDNGTMTAADYARDAN
                     AVLQAKLEKATHEVQLQQAILTYQHTLGNH"
     gene            complement(4049..4681)
                     /locus_tag="PK742_10095"
     CDS             complement(4049..4681)
                     /locus_tag="PK742_10095"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQU76982.1"
                     /translation="MGKTTKTIPAKDQNTEQLILNAARRVFTNKGFAAARMEDIAAEA
                     GMNRALLHYYFRSKEKMFDMIFEENLQLFYGNFLAVLQQDVPFKDKIAMLVSTEIDTL
                     LEHPEIPLFIVQEIARKPEQLKDKMKTIRIHRFVEEFGRQLTEETQRGNIRKVDPRQF
                     FMSFMGMCIFPFIAKPMLKVVMQQKDSEFARLMEERKKEVTRTLLLSLEK"
BASE COUNT          957 a         1214 c         1234 g         1365 t
ORIGIN      
        1 accagcgcca ggttgaagta gcgactggca tccagtctct ccgtcagctg acgggtggct
       61 tcatccggtt cggtgaccag tatcccgatg tcggcattct tgatctcgtt ggtgagggca
      121 aatccgaaga tgatgatctg cacgatgggc agggccatga tgatgagcag cgacttcgga
      181 tcgcggatga tgtggaggaa ctccttgtaa acaaatgtcc ggaactgctt catgttattc
      241 acttctgacg gcgcctctgg ccaggatgcg gaacacctca tccatgcttt gggcaccgta
      301 ttgttctctg agttttttag ggtgatctag tgcttctatg cgaccgtcca ccatgatgct
      361 gacacggtcg cagtactctg cttcatccat atagtgtgtg gtcacgaaga ccgtcatacc
      421 ggtacgtgct gtttcggtga tcatctccca gaaccgtcgc cgggtgatgg gatccacccc
      481 tccggtcggc tcatccagaa agacgatggc cggtttgtgt atgatggcca gtgagaatgc
      541 cagcttctgc ttccatccca gaggcaatgc cttgaccagg gtattcgctt catgggtcag
      601 ttcgagccga tccagcaatg ctttggcttc ttttctgatg gatgtgatgg gcagtccgta
      661 gatgccggcg tagaagcgga tgttctccat cacggtcagg tcttcatata aggagaaccg
      721 ctgactcata tacccgatat gttgtttgat gtgctcggct tcacggtaca catcaaagcc
      781 tgctacactt gcttctccgc tgctgggccg caggatgccg atgagcattt tcatagctgt
      841 ggtcttgccc gctccgttgg caccgagaaa accgaagatc tcgccgggat gcacttcaaa
      901 gctgatgtga tctaccgcag tgaaggtgcc gaactggcgg gtgaggtcgc gaacggtgat
      961 aactggtttc atatcttata agtaggccat gaaacaatct tcaataccgg gcgtgatggc
     1021 ttccatctct acgccgttta tgtttttttc gtccaggaat ttccggacac tttccacgct
     1081 gtattgctca ctttgcaggg taaggtgcag ggcttctcca aaagcaaaac aggaatagct
     1141 gccttcccat tcacgcagtg catgcagtac ttccggactg ttgtttccgg tcaccgcata
     1201 gatgggttta ttgtaggagg cgatgatgtt atcgggtgta tccacctgca gcagggtgcc
     1261 gtgctgcatc agcgcaaccc ggtcgcactg catggcttcg tccatatagg gtgtggagac
     1321 cagcaccgta atacccaggg ctttcaggcg gtgcagcatc tcccagaatt ccttgcggct
     1381 cacaggatcc acaccggtgg tgggttcatc gagcagcaac acttccggct tgtggatgag
     1441 tgcacaacac aaagccagtt tctgtttcat accacctgac agttttcccg cccttctctt
     1501 tttgaaaggc tcgagctgta tatagatatc tttcaccagg tggtagttat cttccagtgt
     1561 ggtgttgaac aaggtggcga agaaacgaat gttctcttcc acgctgagat cctgataaag
     1621 actgaatttg ccgggcatgt aaccgatgag cttccgcacc ttcatataat ctttcagcac
     1681 atccagtcct tccattgtga tgctgccgct gtcaggcaac tgcaggctgg cgatgagccg
     1741 gaagagggtg gacttgcctg ctccatcggg accgatcagt ccgaacagtt ctcccttctc
     1801 aatgtcgagg ttcacaccct gcaatgcctg cacagggggc tttcccggaa aggttttaat
     1861 gatgttatgt acagtgatgc ttttcattac cagctgactt ctccgtacat accgatcttc
     1921 agtttgccat cgttgggcac actgatacga atggcataca cgaggttggc tcgttcatct
     1981 ttggtttgaa tggtcttggg cgtgaattct gcttcttcgc tgatccagat gatggaacct
     2041 tcctgctggt tgaagcctcc gctgccattg tcggtagata cggtcacctt ctgtccgatg
     2101 gacagagttg ccagctgatc gccggtcaca taggcacgca gttccatgtg gctcagatca
     2161 gcgatgcggt acagtggttt ccctgccacc gcgatctcac ctgcttccac ataggaggta
     2221 agaacagttc cattgatggg atttaccaca tgacatttgt tgagcaggtc gttgacctgt
     2281 tctatctgca ggctgagtgg tgctacctct gattgtatgc tgctggtcgt ggtctggagt
     2341 gctgagcgtt gtgcggccag ttgtttttcc agcacatcga tctgtgcatt cgcatcatcg
     2401 agttgcttct gggtggcagc tccgcctttg accagattct cgagtctgtt cagttcaaag
     2461 ttggcggtct tgatctgttc ctgcagggca gcaagctgcg tctgcacatc gggttgtctg
     2521 ctcagtaccg ccttgatctg ggcttccagc tgggcttttt tcagcaccaa ttgggccgag
     2581 tcgatctctg ccacacgtgc attggcttcc agcatctgtc cttcgtcaac agcatagtcg
     2641 atcagtttgc cggtctgttc tgcagatacg atcacctctc tggcttcaaa ggtgccagtg
     2701 gcatcaaatg ctttctcttc gccgttgcag gcgatgaaga agggaacaaa taaaatgaga
     2761 tattttttca tgtgatcagt gatttcctaa tgtatgttga taggtaagaa tggcctgttg
     2821 aagttgtact tcgtgggtag ctttttccag cttcgcctgg agaactgcat tggcatcccg
     2881 ggcgtaatct gctgcggtca tggtgccgtt gtccatcgcc actttgctgc tgctcagcac
     2941 ctcttcccga agcgcaatga tctggtcatc tgtactgagc acgccctgaa gcctgttcac
     3001 ttcttcgtgt tgtctcaccg acaagacctg gatgctgctg ctcatgtttt gtttttgcag
     3061 ggcaagctgt tcctgctgta tgcgccaggt ttctgcttca ttcctgtttt ttcctcctga
     3121 ccataacggg tagttaagcc ttacaccgag catataaaag gtgtcaaatt catcgaccag
     3181 atagttcaac ccgggtcttc cgtatccgcc ctgtacaaag gcgctcactc tgggaagtac
     3241 cagtgcgtcg ctgaaacctt cgccggcctg agcgagctct atttgcttgt cgatcagatc
     3301 cagttccggc cggtgcagtt cggcacttcc gggcatggca ttaccgggag ggatcagttg
     3361 tgtatcgtca ggaatggcaa gtcctgtcag ttgccccagg gcccggatgg caccttgttc
     3421 catggatcgg gcatcatcca tacgctggtt gagccgcagg atctctgctt ccagtgtttt
     3481 tacctgtgct gctgtggctg taccattctt atacgctgtt tccagttgtc ccttgcggtt
     3541 ctccaggtct ttcttcgtac tgtagatcag cagttgttgt tgcctggcaa ggagtatacc
     3601 cgtgaacaac tggtctatct ggtcatagat ggaatagaac tgtgaactca cggatgcaga
     3661 ttctactgct gcctgcaggt cgctcatggt ctttttctgg tgcaccagtc cgccgtcata
     3721 caaggtttgc gtaacatcta tcgtagcccg gtactggtct ttgctgagtg taggtatctc
     3781 gaacagcggc gactccagcg gaatgcccac cacgtcactt tgataggtgg cctgggcatt
     3841 gaggttcacc tgtggttgcc agtagctgcc tgccactttc tgttgcgctt cattcttgtg
     3901 gctgatcagg gtctgctgtt cgtccagtga gaacttagct tcagcagccc gatagcattt
     3961 ttgcagatcg agcagagcag gatcctgagc cgatgccagc atggggatga tcagcaaaaa
     4021 gttccatata gcatgtttct tcatatgcct atttttccaa tgatagtaag agggtgcggg
     4081 tcacttcttt ttttcgttct tccatcagac gagcgaactc ggagtctttc tgctgcatga
     4141 ccaccttcag catgggtttg gctataaagg ggaagatgca catgcccatg aacgacatga
     4201 agaactgacg tggatcgacc tttctgatat tgccacgctg cgtctcttct gtgagctgtc
     4261 tgccaaattc ctccacaaat cggtggatgc ggatggtctt catcttatcc ttcagctgtt
     4321 ccggtttgcg ggcgatctcc tgcacaatga aaagaggtat ctccggatgt tccagcaggg
     4381 tatcgatctc tgtgctgacc agcatggcga tcttgtcttt gaagggcaca tcctgttgca
     4441 gtactgccag gaaattcccg tagaataact gcaggttctc ttcaaagatc atgtcgaaca
     4501 tcttctcctt actgcgaaag taatagtgga gtagagccct gttcatacct gcttccgctg
     4561 caatgtcttc catgcgggca gccgcgaatc ccttattggt gaacaccctt ctggcagcat
     4621 tcaaaatgag ctgctctgtg ttctggtctt tggccggtat cgttttggtc gttttaccca
     4681 ttggtttaac aatattgtta aacatttgtg caaaggaact ttgcctggtc cgtttttcca
     4741 aatttttttt gtgaagagat cttaaaactt
//
LOCUS       DAOMAX010000209         4760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3101_length_4760_cov_3.672689, whole genome shotgun sequence.
ACCESSION   DAOMAX010000209 DAOMAX010000000
VERSION     DAOMAX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_4760_cov_3.672689"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..2924)
                     /locus_tag="PK742_10100"
     CDS             complement(66..2924)
                     /locus_tag="PK742_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76983.1"
                     /translation="MNQYFLRLSMAVLLSGSTIVLQAQTPAGMRMSGINKRQVLEEHS
                     LVSQVPFRNIGPSIMSGRVTDLAVNPDKPEEFYVAYASGGLWYTNNNGQSFQPLFQQE
                     DVMTIGAIAVNWETGVIWVGTGEVNSSRSSYAGIGMYKSADRGQSWQYAGLPESHHIG
                     SVVMHPDDPNTVWVAVLGHLFTTNPERGVYKTTDGGEHWKKVLFVDDKTGAVEVTIDP
                     VHPERLYACMWQRDRKAWNFTEAGSGSGIWGSEDGGDNWKLLTGSNSGFPSGEGMGRS
                     GLAIFPGNTNVLYAVVDNQSADPSKATEQDTSVMTMNDLKDITKEKFLALDDDKLNAF
                     LQQQGVDEKFTAAYLKDQVQSGAFRPTVLNEYLNLGAYVFNLPIHGCEVYKSTDRGEH
                     WVRVNETDLGSMYYTYGYYFGKIYVSELNSDLVYILGVPVAMSTDGGKHFTEIGKGNV
                     HADHHVLWVDPADDGHLILGNDGGGNITYDHGQKWFKANDPPVGQFYSVTVDDAKPYH
                     VYGGLQDNGVWSGPSDYQADDSWLQDGRYPFRFLYGGDGMQVQVDTRDNKTFYTGYQF
                     GFYARMNLDDDNDYMEIRPRHEMGEYPLRFNWQTPILLSRHNQDVFYYGSNRFHRSLN
                     KGEMLETLSDDLSGGGEDGDVPYGTITAISESPLRFGLLYAGTDDGHVHLSVDGGYTW
                     NEISAGLPPGLWVSRVVASAHELNRVYLTMNGYRNDDFRSLVFVSNDRGKSWKPLATD
                     LPAEPVNVIREDAVNPSLLYIGTDNGLYISLNGGVSCMAFMGTGEQRLPRVAVHDLAI
                     QEREQELVVATHGRSLFVADISHVRALNDSLLNVPLHLFPHDKMPWRSGQGNGSSWNT
                     LAEPYVQTTELVACSKTATSATLQVMLDTIVLYTRQVVLMPGLNYLEYDLSAATPPDN
                     WKAADNGVWYLQPGVYSVHLSTEGMEAQPIELELVEKR"
     gene            2980..3753
                     /locus_tag="PK742_10105"
     CDS             2980..3753
                     /locus_tag="PK742_10105"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HQU76984.1"
                     /translation="METKEAIVRNWLPRYTGTPLEAFGEYILLTNFINYVDSFAEKFG
                     VEVKGKDKPMQTATSNGITIINFGMGSAMAATVMDLLSAVMPKAVLFLGKCGGLKKNN
                     EVGDLLLPIAAIRGEGTSNDYMPEEVPALPSFRLQMAVSHVIRRYEQDYFTGTVYTTN
                     RRVWEHDQEFKEYLRSIRAMAIDMETATIFTVGFINSIPHGALLLVSDIPMDPEGVKT
                     AESDRKVTSNFAETHLSIGIDALLELRDSGESVKHLRFE"
     gene            complement(3756..4535)
                     /gene="cysQ"
                     /locus_tag="PK742_10110"
     CDS             complement(3756..4535)
                     /gene="cysQ"
                     /locus_tag="PK742_10110"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQU76985.1"
                     /translation="MLDKIQLQEIIDIAEEAGKAIMEIYSTDDFAVVDKSDNSPLTKA
                     DKASNEVIIRRLQSSYPEIPIISEENKEVPYADRKDWTWFWLVDPLDGTKEFIKKNGE
                     FTVNIALVHDGRPVLGVVGIPARNEMYYAVQGQGAWKIDAEGNKHRLQVNEPSAERIA
                     LIGSRSHPSPEFDAYLNDMESKYAQVDFVPAGSSLKFCLVAEGKADVYPRLGPTMEWD
                     TGAGHAVVLEAGARVRIHGASEDLSYNKENLLNPFFIVERA"
     gene            complement(4528..>4760)
                     /locus_tag="PK742_10115"
     CDS             complement(4528..>4760)
                     /locus_tag="PK742_10115"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HQU76986.1"
                     /translation="KGAITWFDLAAAVMDTYGLNCKVNPIPTSSYPTPAKRPAYSVLD
                     LSGTASVPGMEIPDWKTSLQQCIEEIKTLENA"
BASE COUNT         1175 a         1258 c         1105 g         1222 t
ORIGIN      
        1 cggggttttt tctgattaga acaaaatatt tcccgtagtt gcccacaacc tgcttgtgaa
       61 ctgtcttacc ttttttcaac taattcaagt tcaattggtt gtgcctccat tccttctgta
      121 ctgagatgga cagaatacac tccaggctgc agataccata cgccattatc agcagctttc
      181 cagttgtcag gcggtgtggc ggcgctcaga tcatattcca ggtagttcaa tcctggcatg
      241 agcaccacct gccgggtgta cagcactata gtgtccagca tcacctgcag ggtggcactt
      301 gtggccgttt tactgcaggc caccagttct gtggtttgta catagggctc agccagggtg
      361 ttccaggacg aaccgttacc ctgtcctgac ctccacggca tcttgtcgtg cggaaaaagg
      421 tgcaaaggca cattcagtaa agagtcattc aatgctctta catgactgat atcagctaca
      481 aaaagactgc gaccatgggt tgccaccacg agctcctgtt cccgctcctg aatggccagg
      541 tcatgcactg ccactctggg caagcgctgt tcacctgtgc ccataaaggc catgcagctg
      601 actccgccat taagcgaaat gtacaggcca ttatctgttc ctatataaag cagtgaggga
      661 ttgacagcat cttccctgat cacattgacg ggttcagcag gcaggtctgt agcgagaggc
      721 ttccagcttt tgccacgatc attgctgaca aaaaccaatg acctgaagtc gtcattcctg
      781 taaccgttca tggtcagata taccctgttc agctcatggg ctgaagctac taccctgctg
      841 acccacagac ccggcggaag tccggcagag atctcgttcc aggtgtaacc accatcaacc
      901 gacaaatgta cgtgaccatc gtctgttccg gcatacaaca gtccaaaacg aagcggagat
      961 tcactgatgg cagtgatggt accgtaaggc acgtcaccgt cttccccccc tccgctcagg
     1021 tcatcactca gcgtttccag catctcaccc ttgttcagtg acctgtgaaa gcggttgctt
     1081 ccgtaataaa aaacatcctg attgtggcgt gacaagagga tgggcgtctg ccagttgaat
     1141 ctcagcggat actcacccat ttcatgcctg ggcctgatct ccatgtaatc attgtcatca
     1201 tccagattca ttctggcata aaagccaaac tggtatccgg tataaaaggt cttgttgtct
     1261 cgggtatcta cctgtacctg catgccatcg ccaccgtaca ggaaccggaa cggatacctg
     1321 ccatcctgta accaggaatc atctgcctgg taatcagacg gacctgacca gacaccatta
     1381 tcctgcagac ctccgtaaac gtgatagggc tttgcgtcat ctactgttac gctgtagaat
     1441 tgcccaacgg gtggatcatt ggccttgaac catttctgac catggtcata ggtgatgttg
     1501 cccccgccgt cattacccag gatcagatgc ccgtcatcgg caggatctac ccacaataca
     1561 tggtgatctg catggacatt tccttttccg atctctgtaa aatgctttcc gccatcggtg
     1621 ctcatcgcca ccggcacacc cagaatatac accagatctg aattcagctc tgacacatat
     1681 atctttccaa aataataacc ataggtatag tacatgctcc ccaggtctgt ctcattgacc
     1741 ctgacccagt gttcgccgcg atcagtgctc ttatacacct cgcatccgtg aatgggcagg
     1801 ttgaacacat aggcacccag gttgaggtat tcattcagaa ctgtggggcg aaaagccccg
     1861 ctttgcacct gatctttcag ataagcggcg gtaaactttt catcaacgcc ctgctgttgc
     1921 aaaaaagcat tcaacttgtc atcatccagg gcaaggaact tttctttggt gatgtctttc
     1981 agatcattca tagtcatcac actggtgtct tgttctgttg ctttggaagg atctgcagac
     2041 tggttatcga ccactgcata cagaacattg gtatttccgg gaaatatggc aagacctgat
     2101 cttcccatcc cctctccgga agggaacccg gagttacttc cggtcagcag tttccaatta
     2161 tctccgccat cttcagaacc ccagatacca gacccggacc cggcttcagt aaagttccag
     2221 gcttttctgt cacgctgcca catacaggca tacaaacgtt caggatgcac cggatctatg
     2281 gtcacctcaa cagctcctgt cttatcatct acaaacaata ctttcttcca gtgttctcca
     2341 ccatctgttg tcttatatac accgcgttcg gggttggttg taaagaggtg acccagtacg
     2401 gctacccaca cagtgttcgg gtcatcggga tgcatgacca cgcttcctat atgatgcgac
     2461 tcaggcagtc cggcatactg ccaggattgt cctctgtcag cactcttgta catgcctata
     2521 ccggcatagg atgacctgga agaatttacc tctccggtgc ctacccagat cacacctgtc
     2581 tcccagttga cggcaatagc acctatggtc atcacatctt cctgctggaa cagcggctga
     2641 aaggactggc cgttattgtt cgtgtaccag agtccgccac tggcataggc tacatagaat
     2701 tcttccggct tgtcagggtt cactgcgaga tctgtaaccc tgccgctcat aatggacggg
     2761 ccgatgtttc tgaacggcac ctggctaaca agggagtgct cttccagaac ctggcgctta
     2821 ttaatgcctg acatgcgcat accggcagga gtttgtgcct gcagaacaat ggtagatccg
     2881 gacaacagga cggccattga taaacgaagg aagtattggt tcatatctgt aaaaataggc
     2941 aaagcgggaa ggtttacctt tggatggaaa gtttgtagta tggaaactaa agaagcgatc
     3001 gtcagaaact ggctccccag atacaccggc acaccactgg aagcctttgg agagtatatt
     3061 ctgctgacca atttcatcaa ctatgtggat tcatttgctg agaaattcgg agtggaagtg
     3121 aagggaaagg ataagccaat gcaaactgcc acgtctaacg gcatcaccat catcaatttc
     3181 gggatgggtt ctgccatggc cgcgaccgtg atggacctgc tgagtgcggt catgccaaaa
     3241 gcggtgttgt ttcttggtaa atgcggtgga ctaaaaaaga acaacgaagt gggcgatctg
     3301 ctgctgccca ttgcagccat cagaggagaa ggaacaagca acgattacat gccggaggaa
     3361 gtgcccgccc tgccctcatt ccggttacag atggcggtca gtcacgtgat cagacgctat
     3421 gaacaggatt actttacagg cacggtctat acaaccaacc gcagggtgtg ggagcatgac
     3481 caggaattta aagagtacct gcgaagcata cgtgccatgg ccattgacat ggaaacagcc
     3541 accattttca cggtaggatt cattaacagc ataccacacg gagcgctgct gctggtatca
     3601 gacataccaa tggatccgga aggtgtgaaa acggcagaaa gcgaccggaa ggtcacctcc
     3661 aattttgccg aaacacacct gtcaataggc attgatgctt tgcttgaatt acgtgattca
     3721 ggtgaatcgg tcaagcacct ccgcttcgaa taagatcagg ctctttctac gatgaagaaa
     3781 ggattcagaa gattctcctt attgtatgac agatcttctg aagcaccgtg tattcttacc
     3841 ctggctcctg cttcaagcac cacagcatgg cctgcaccgg tatcccattc catggtgggt
     3901 ccgagccgcg gatatacatc agcctttcct tcagcaacca gacagaattt cagtgagctt
     3961 cctgcaggta cgaaatcaac ctgtgcatat ttcgattcca tgtcattcag gtaagcatcg
     4021 aattcaggcg acggatgcga acgactgccg atcagggcta tgcgttctgc cgatggttca
     4081 ttcacctgca gcctgtgttt attgccttct gcatcgatct tccaggcacc ttgtccctgc
     4141 acggcatagt acatttcatt acgggcgggt attcctacca ctccaagaac aggacggcca
     4201 tcatgcacca gggctatgtt aacggtgaac tctccattct tcttgatgaa ctctttggtg
     4261 ccatcgagcg gatctaccag ccagaaccag gtccagtctt tacggtcggc atagggaact
     4321 tccttgtttt cttctgaaat gatgggaatt tcaggatagg atgattgcag ccttctgatg
     4381 atcacttcat tcgaggcttt atctgctttg gtcagcggcg aattatcgct tttgtccaca
     4441 actgcaaaat cgtcggtaga atagatctcc ataatagcct ttccggcttc ttccgcaatg
     4501 tcaatgatct cctgtagctg aatcttgtca agcattttct agtgttttta tttcttcaat
     4561 acattgttgc aaacttgttt tccagtccgg aatttccatt cccggaaccg atgccgtacc
     4621 tgacagatcc agcacggaat aggccggtct tttagccggc gtaggataag atgaagtggg
     4681 aataggattc accttgcagt tcaacccata ggtatccata acggcagcag ccagatcgaa
     4741 ccaggtgatg gctcctttgt
//
LOCUS       DAOMAX010000210         4713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3150_length_4713_cov_3.898454, whole genome shotgun sequence.
ACCESSION   DAOMAX010000210 DAOMAX010000000
VERSION     DAOMAX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_4713_cov_3.898454"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PK742_10120"
     CDS             complement(<1..743)
                     /locus_tag="PK742_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU76987.1"
                     /translation="MSVKLTRNKKLMAYAAMAAAVPAGKVTGQVIYHDLDPDLIVENS
                     FCYGCTDVEYIDMDMDGSDDFAIVMFYDYGLSGYLPTHSAYLMPLGSNKVAYAIDSST
                     VWNYDLGGYVLMTENVAQKLSFGDVVDTSYNFSTLNAVVRSYIEGYDEFTSPTFGQWA
                     VNQTAYAGVQLMIDGEPHYGWIKLTHAHPGFDWQLSALEYAYQATTETSLVVGEIPVS
                     CDPPVLSSVITLPGSMKLFFEPEAEASQY"
     gene            complement(811..1902)
                     /locus_tag="PK742_10125"
     CDS             complement(811..1902)
                     /locus_tag="PK742_10125"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQU76988.1"
                     /translation="MKGSVKFVERPKTDFFNVLRSRVNEYFDQHRIERTGDYRMVMKS
                     VAMFTLYLAPFAVILTGVLPAWATLLCWVLMGLGMAGVGMSVMHDANHGAYSRYPWIN
                     NLMGRTIYLLGGSRFTWKIQHNILHHTYTNIYGLDEDIHDKPILRLSPHGRLGFLHRY
                     QHVYGFLLYGLATLGWTINKDFKQLIRYNKVGLTVSSGGDPTIEMIKLIASKAGYYLV
                     LMGLPLWLSGYSFGWILLGFLIMHFTSGLVLTTIFQLAHLVEDMDHPQPEDGVIANGW
                     AIHQLQTTSNFARKNKILSWYVGGLNYQIEHHLFHNICHIHYDKIAPIVRDTAREYGI
                     EYHEMETFWQAVGSHWRVLRQIGQNSLAA"
     gene            complement(1988..3787)
                     /gene="typA"
                     /locus_tag="PK742_10130"
     CDS             complement(1988..3787)
                     /gene="typA"
                     /locus_tag="PK742_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQU76989.1"
                     /translation="MSAFRNIAIIAHVDHGKTTLVDKILHHCQTFSKETNELILDNNE
                     LERERGITILSKNVSVHYKGVKINIIDTPGHADFGGEVERVLNMADGALVLVDAFEGP
                     MPQTRYVLQKALSLGLKPVVVINKVDKDNCRPDEVHEAMFDLMFNLGATEDQLNFPTA
                     YGSAKHGWMAGDWKQPATDIAFLLDTILEYVPAPEERTGTVQMQITSIDYSNYIGRIA
                     IGRVHRGTLSAGQAVSLVKRDGSIVKSRVKEVFLFEGLGKAKADTITCGDICAVTGLE
                     GFEIGDTVADIETPEALPTIPIDEPTLSMLFTVNDSPFFGKEGKFVTSRHLRDRLYKE
                     MEKNLALKVVETNSPDAFMVFGRGIMHLSILVETMRREGYELQLGQPRVIIRNIDGVK
                     SEPVEELVIDTTEETAGKVIELVSQRKGEMLVMEPKGDMTHLEFRIPSRGIMGLRTQV
                     LNSTQGEAVMNHRFKDYEPWKGEIPSRINGSLIVHETGTTIAYAMDKLQDRGIFFVGP
                     GEEVYLGQVIGEHSRDNDLGVNVTKTKKLTNMRASGSDDKVKLAPPRRMSLEEAMEYI
                     QADEYVEVTPVNIRLRKIHLLEHERKKAALVAE"
     gene            3982..>4713
                     /locus_tag="PK742_10135"
     CDS             3982..>4713
                     /locus_tag="PK742_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU76990.1"
                     /translation="MGMKWMWMTTFLLASVCVQAQELNGKITDEKTGEVIPYVNVGVA
                     AKGIGTVSQGDGRFHLNIDSAGTTDTLVVSFIGYTSKKFAISELRQGSGYLSVQLEQQ
                     VFDLNEVEVRPVRLIPVVLGTDFDKPYISAGFKSNDLGSEVGMVMKTKKDRKYYLNST
                     GFWISKCEYDSILFRVNVYDYNKGEVGELLHQLPIYVKATPATKHMQIDLRPYNISVT
                     NDFVITLEWIQELPDPMESFMFCAGF"
BASE COUNT         1191 a         1189 c         1121 g         1212 t
ORIGIN      
        1 ttgtattgag aggcttctgc ttccggttca aagaacaatt tcatggagcc gggaagtgtg
       61 atcacagacg aaagtaccgg aggatcgcag gatacaggta tttcaccaac taccaggctg
      121 gtttctgttg ttgcctgata ggcatattcc aaagcagaca actgccagtc aaagcccgga
      181 tgtgcatggg tcagtttgat ccagccgtaa tgcggttctc cgtctatcat cagctgaaca
      241 ccggcatagg ccgtctgatt aacagcccac tgaccaaaag taggagaagt aaattcatca
      301 tatccttcta tataacttct tactacggca ttcagggtag aaaaattata ggaagtatca
      361 accacatctc caaaactcag cttctgagcc acattctcag tcatgagcac atagcctccc
      421 agatcatagt tccatacagt actggaatcg atagcatagg ctactttatt acttcccaat
      481 ggcatcaggt aggccgagtg tgttggcaga taaccgctca gtccgtaatc atagaacatc
      541 acaatggcaa aatcatcaga accatccata tccatatcta tatactccac atctgtacaa
      601 ccatagcaaa aggaattttc tacaataaga tccggatcca gatcatgata gatgacctga
      661 ccggttactt tgcctgcagg tacagcagct gccatagcgg cataggccat taatttcttg
      721 ttacgggtaa gcttcacaga catgtatatc agatttgatg tatctctaag atacaataaa
      781 tcatatagat gaagctaatg aagtaagatc tcaggcagca aggctgttct gtcctatctg
      841 acgcaacaca cgccagtggc ttcccacagc ctgccagaaa gtttccatct cgtggtattc
      901 aatgccatac tctctggctg tatcacgaac aatgggtgct atcttatcat aatgtatatg
      961 acatatatta tgaaacagat ggtgttctat ctggtagttc aatccgccca cataccagga
     1021 aagtatctta ttcttccggg caaaattcga ggtggtctgg agctgatgta tggcccagcc
     1081 gttggcaatg acaccgtctt ccggttgcgg atgatccatg tcttccacca ggtgcgccaa
     1141 ctgaaagatg gtggtcagca ccagaccgga ggtaaagtgc atgatgagaa atcccagcag
     1201 tatccagcca aaactataac cggacaacca cagcggcaag cccatcaaca ccagatagta
     1261 tcctgccttg ctggctatca gtttgatcat ttcgatggta ggatcacccc cactgctaac
     1321 agtcagacca actttattgt aacgaataag ctgtttgaag tctttgttga tggtccagcc
     1381 cagagtggcc agtccgtaca ggaggaatcc gtacacatgc tggtatcggt gcagaaatcc
     1441 gaggcggcca tgcggcgata aacgcaggat gggcttatca tggatatctt catccaggcc
     1501 gtaaatattg gtataggtat ggtgaagaat gttatgctgg atcttccagg taaagcgact
     1561 gcccccaagc agataaatgg tgcggcccat caggttgttg atccatggat aacgggaata
     1621 tgcgccatga ttggcgtcgt gcatgacaga catgcccaca cctgccatac caagccccat
     1681 cagcacccag cacaataagg ttgcccaggc tggcaataca ccggtaagga tgacagcaaa
     1741 tggcgccaga tacaaagtga acatggccac cgatttcatc accatccggt aatcgccggt
     1801 gcgctcaata cgatgctggt caaaatattc gttgacccgg ctgcgcaata cattgaagaa
     1861 gtccgtcttc ggcctctcca caaactttac agaacctttc aatacagtta attgatacga
     1921 aaaggcaaag ataactgccc gaaactggta attatcggat cattgacaat aatcaccgga
     1981 atcgtcctta ttctgctaca agagccgctt ttttccgctc atgctccagc aggtggatct
     2041 tgcgcaggcg gatgttcacc ggcgtcactt ccacgtattc atcggcttgt atgtattcca
     2101 tggcctcttc gagcgacata cgcctgggcg gagccagttt gaccttgtcg tcagaaccgc
     2161 tggcccgcat gttcgtaagc tttttcgtct tggtcacgtt gaccccgagg tcgttgtcgc
     2221 gactgtgttc accgatcacc tgacccagat agacctcttc tcccggacca acaaaaaaga
     2281 tgcccctgtc ctgcagcttg tccatcgcat aggcaatggt cgtgcctgtt tcatgaacga
     2341 tcaaagatcc gttaatgcgg ctgggtatct ctcctttcca gggttcgtaa tccttaaagc
     2401 ggtggttcat cactgcttca ccctgggtac tgttcagcac ctgggtgcgg aggcccatga
     2461 taccccggct ggggatgcgg aattccaggt gggtcatatc gcccttaggt tccatgacca
     2521 gcatctcgcc cttgcgctga ctgaccagtt cgatgacctt accggcggtc tcttcggtgg
     2581 tgtcgatgac cagttcttcc accggttcac tcttcacacc atctatgttg cggatgatga
     2641 ccctcggctg accaagctgc agttcatacc cttcccgccg catggtctct accaggatag
     2701 acaggtgcat gattccccgg ccgaatacca tgaaggcatc aggagaattg gtttccacca
     2761 ctttcagtgc caggttcttc tccatctctt tgtacaggcg gtcacgcagg tggcgactgg
     2821 tcacgaattt cccttccttg ccaaagaacg gcgaatcatt cactgtgaac agcatactca
     2881 gtgtcggttc atcgatcgga atggtgggca atgcctctgg tgtttcgata tctgctaccg
     2941 tatcgccaat ttcaaatccc tccagtccgg tcacggcaca gatatcgcca caggtgatgg
     3001 tatctgcctt ggcctttccc agtccttcaa acagaaagac ctctttgacc cggcttttaa
     3061 cgatggaacc atcccgcttc acaagactta ccgcctggcc tgcagacaat gtacctctgt
     3121 gtaccctgcc gatagcaatc cgtcctatat aattggagta atcaatactg gtgatctgca
     3181 tctgtacagt accggtgcgt tcttcgggag caggtacgta ttccaggatg gtatcgagca
     3241 aaaaggcaat atcggttgca ggctgcttcc agtcgccggc catccagcca tgcttggcgc
     3301 tgccgtaggc agtggggaag ttcagctggt cttccgttgc acccaggttg aacatgaggt
     3361 cgaacatggc ttcgtgcact tcatcgggcc gacagttatc cttgtccacc ttattgatga
     3421 caaccacagg cttcagcccc aggctcagcg ctttttgcag cacataacgg gtctggggca
     3481 tcggtccttc gaaggcatct accagcacca gtgcaccatc ggccatgttg agtacccgtt
     3541 ccacttcccc gccaaagtcg gcgtgacccg gtgtgtcaat gatgttgatc ttgactcctt
     3601 tatagtgaac agatacgttc ttggacagaa tggtgatgcc tcgctcccgc tccagttcat
     3661 tgttgtccag gatcagctca ttggtttctt tgctgaaggt ctgacagtga tggaggatct
     3721 tgtcaaccag ggtcgttttg ccatggtcca cgtgggcaat gatggcgatg tttctgaatg
     3781 cgctcatgta attcttgaaa agaggtgcaa aggtaggggt tatttggtta taagttattg
     3841 gtcgttggta tttagttatt ggctgtttgt tgctggtatt gggttgttgg tcattgctta
     3901 cgggtaacca ataactaatt accggcaacc aaagaccaaa gaccacattc ccctgtaaca
     3961 aatccctctt aacttcgtct tatgggcatg aaatggatgt ggatgacaac tttcctcctg
     4021 gcatcggtat gtgtgcaggc gcaggagctt aacggaaaaa taacagatga aaagaccgga
     4081 gaggtcatac cctatgtgaa tgtaggtgtc gcggcaaagg ggatcggtac ggtcagccag
     4141 ggcgacggca ggtttcatct gaatattgat agtgcaggaa ccacagacac attggtagtt
     4201 tcctttattg gttataccag caaaaaattt gcgatctcag aactgagaca gggttccgga
     4261 tatctttctg ttcaactgga acagcaggtt ttcgatctga atgaggtgga agtgagaccg
     4321 gtcagactga tacctgtggt tctgggtaca gattttgata aaccatatat ttctgccgga
     4381 tttaaaagca acgacctggg ctctgaagtg ggcatggtca tgaagaccaa aaaagaccgc
     4441 aaatactatc tcaacagcac cgggttctgg atttcgaaat gcgaatatga cagcatcctg
     4501 ttccgggtca atgtctatga ttacaacaag ggagaagtgg gcgaactgct gcaccagctt
     4561 cccatctatg tcaaggccac tcccgcaaca aaacatatgc agatagatct gcggccatac
     4621 aacatctctg tgaccaacga ttttgtcatc accctggaat ggatacagga actgcccgac
     4681 cccatggaaa gtttcatgtt ctgtgccggt ttt
//
LOCUS       DAOMAX010000211         4702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3164_length_4702_cov_3.128900, whole genome shotgun sequence.
ACCESSION   DAOMAX010000211 DAOMAX010000000
VERSION     DAOMAX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_4702_cov_3.128900"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PK742_10140"
     CDS             <1..1068
                     /locus_tag="PK742_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQU76991.1"
                     /translation="RDFTINAMAIALNKVRFGQLIDPFNGMQDLHDGRIRTPQEPAAT
                     FSDDPLRMMRAIRFASQLGFHIELHTLEAIAAEKKRIEIISAERITDELNKIILSPVP
                     SAGFLLLFQTGLLAIIFPEFQELHGVENMEGQSHKDNLYHTLEVLDNLSRVSDDLWLR
                     WGAVLHDIGKPATKRFQQGHGWTFHGHEVVGARMVKKIFRRMKLPMNEQMKFVEKMVL
                     LHLRPIALHQDEVTDSAIRRLLFDAGEDIGQLMLLCEADITTKNRNRMKRYLKNFELV
                     RQKLVEVEEKDRITNFQPPVSGELIMQTFGIGPGKEVGAIKNHIREAILDGIIPNEYE
                     PAYQLMLEKGKELGLTTSAEK"
     gene            1082..3208
                     /locus_tag="PK742_10145"
     CDS             1082..3208
                     /locus_tag="PK742_10145"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU76992.1"
                     /translation="MNLLRGQATYLLLLLAIFSASAQSPDLVWQHTIGGSNDDRGLWI
                     EQSADGGYFLGGHSTSDISGEKSENNIGIPGSYDYWVMRLDADGNIVWQNTIGGAEDD
                     QFDVLRATPDGGCIIGGSSHSNASGDKTSNVVGEYDYWIVKLDADGNIQWQQQYGSDD
                     SEVLTDLNLTDDGGYIIGGISGGNISGDKTEDARGDVDYWIIKTDHLGNIVWQRTIGG
                     NKEDYLYTADQTSDGGFIIGGSSKSTPSGERTLPLTGTSDMWFLKLDASGNIMWQNHI
                     GSNVKDRLNALFETADGGYMIGGYSAGGVFADKTENSLFIDYWILKLDDAGDILWQNT
                     IGGPDLDLLNTARPAPDGGMIAVGSSTSNAFADKTEDAFGGLDYWIVKVDDAGNVSWD
                     KTLGGSDDDEPFGICVTTDGDYVIAGYSNSGISGNKTEMNRGAGDYWLVKLGNAMVAE
                     PCSTAPEGSFADNITPVSAKLHWLSDAGALKYLVRFRQSGTPAWTAVPSGINNKNISG
                     LMPSTAYEFQVRSFCGPDNKSGWSTSSVFTTSPLRTADTCEASFTYSIVNTGLCELTN
                     TTAGAASCGAPTIFYWEFCGDDPTNVENPYYEFTDTFCICLTVTFTSGFISTTCSDTL
                     LYTALEDIGISDGLFLYPNPVADLLFVSTSDADMRSAWKISDLSGRQMDNGKLHHNIP
                     IDVHALPAGMYVFELDSGSRRVFVKE"
     gene            3240..4142
                     /gene="miaA"
                     /locus_tag="PK742_10150"
     CDS             3240..4142
                     /gene="miaA"
                     /locus_tag="PK742_10150"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQU76993.1"
                     /translation="MSKHLLIICGPTASGKTTLAIALAKHFGTEILSADSRQVYKELK
                     IGVARPTEEELAAVPHHFIATNSIHEPLSAGRYEQEALALLEKLFQQHDVVVMAGGSG
                     LFIKAVTEGLDNLPASHDMKVQLHEELDEKGLEWLQQEVEKLDPEYYASADQQNPRRL
                     LRALEVIRLSGQTYSSLRTGKAKERPFRSHLYLPEWDRDVLYDRINKRVDQMMAEGQE
                     EEARSLLPHRHLSTLHTVGYSELFDHFDGLYDLETAVALIAQHTRHYAKRQLTWFRNE
                     APATPVSHTTQEALEQIITGYEKM"
     gene            4173..4679
                     /locus_tag="PK742_10155"
     CDS             4173..4679
                     /locus_tag="PK742_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQU76994.1"
                     /translation="MIRILLMSDTHGYMDERILEHAAEADEVWHAGDIGPLESIREIR
                     RIKPVRAVFGNIDGREVRQQAPLDNRFELDGLDVWMTHIGGYPGRYEPRVREILKVKP
                     PGLFICGHSHILKVQFDQKYRMLTMNPGAAGMNGFHKVRTMLRFAIDQGEVKDLAVIE
                     LGPRGRTI"
BASE COUNT         1202 a         1205 c         1282 g         1013 t
ORIGIN      
        1 cgcgatttca ccatcaacgc tatggccatt gccctgaata aggtgcgttt cggacagctc
       61 atcgacccgt tcaacgggat gcaggacttg cacgacggtc gcatacgcac cccgcaggaa
      121 ccggcagcaa ccttcagcga cgatccgctg cgcatgatgc gggccatacg ctttgcctcg
      181 cagctgggtt ttcacatcga gctgcatacg ctggaagcca tcgctgcaga aaaaaaacgc
      241 atagagatca tcagtgcaga gcgcattacc gatgagctga acaagatcat tttatcgccc
      301 gtgccctctg ccggattcct gttgctgttc cagaccggat tactcgccat catcttcccg
      361 gaattccagg agctgcatgg cgtagagaac atggaggggc agtcgcacaa ggacaacctc
      421 taccacaccc tcgaagtact ggacaacctg agccgggtaa gcgatgatct ctggctgcgc
      481 tggggggccg tgctgcacga catcggcaag cccgctacca agcgttttca gcagggtcac
      541 gggtggacct ttcacggaca tgaagtggtg ggtgcccgca tggtaaagaa gatcttccgc
      601 cgcatgaagc tgcccatgaa cgagcagatg aaattcgtag agaagatggt actgttgcat
      661 cttcgtccca ttgccctgca ccaggacgaa gtgacggatt cagccatcag aagattgttg
      721 ttcgatgcag gggaagacat cggccagctg atgctgttgt gtgaggccga catcaccacc
      781 aagaaccgca accgcatgaa gcgctacctc aagaacttcg agctggtgcg gcagaaactg
      841 gtggaggtgg aagaaaagga ccgcatcacc aacttccagc caccagtgag cggtgaactc
      901 atcatgcaga cctttggtat cggtcccggc aaggaagtcg gtgccatcaa gaaccacatc
      961 cgcgaagcca ttctcgacgg catcatcccg aacgaatacg aaccggcgta tcagctgatg
     1021 ctggagaagg ggaaggagct gggattgacc acttctgcag aaaagtaggt agatttatta
     1081 catgaactta cttcgcgggc aagcaacata cctgttgttg ctgttggcta ttttttctgc
     1141 ttcagcacaa tctcccgacc tggtctggca gcataccatc ggtggcagca atgatgaccg
     1201 cgggctctgg atcgagcagt cagctgatgg cggctatttc cttggcggac attccacctc
     1261 agatatttcc ggagaaaaat cagaaaacaa tattggtatt cccggttctt acgattactg
     1321 ggtcatgcgt ttggatgctg acgggaatat cgtctggcag aataccatcg gcggggcgga
     1381 agacgatcag ttcgatgtgc ttcgggccac tccggatggc ggatgcatca tcggcggcag
     1441 cagccactct aatgcctccg gtgataagac tagcaatgta gtgggtgagt atgactactg
     1501 gatcgtcaag ctggatgcag acggaaacat acagtggcaa caacaatatg gctcagacga
     1561 ttctgaagta cttacagatc tcaacctgac cgatgatggg gggtatatca taggcggcat
     1621 ttcaggtggc aatatttccg gcgacaaaac ggaagacgcc cgaggcgatg tggactactg
     1681 gatcatcaag acagaccatt tgggcaatat cgtatggcag cgaaccatcg gaggaaataa
     1741 agaagactat ctctatacag cagatcagac aagtgatggt ggcttcatca tcggcgggag
     1801 ctccaaatca accccttccg gcgaacgcac ccttcccctc accggcacca gcgatatgtg
     1861 gtttctgaaa ctggatgcat caggaaatat catgtggcag aaccacatcg gctccaacgt
     1921 caaagaccgg ctcaatgcgc tttttgagac agcagacggt ggctatatga taggtggcta
     1981 ttcggccgga ggcgtctttg cagataagac cgagaacagc ttgttcatcg actactggat
     2041 cctgaagcta gatgatgcag gagacatact ctggcagaat accatcggcg ggccggacct
     2101 cgacctgctc aacaccgcca gacctgcacc cgatggcggc atgattgcgg tcggctccag
     2161 taccagcaac gcttttgcag acaagacgga agatgccttt ggtggactgg actactggat
     2221 cgtgaaggtg gatgatgcgg gaaacgtaag ttgggacaag accctcggtg gcagcgatga
     2281 tgacgaacca tttggtattt gtgttaccac cgatggtgat tacgtgatag caggatattc
     2341 caactcaggt atcagtggta ataaaactga aatgaaccgt ggtgccggcg attactggct
     2401 ggtgaaactt ggtaatgcga tggtggcaga accatgcagc acagcacctg aaggatcgtt
     2461 tgcagacaac atcacaccgg tctctgccaa actgcactgg ttgtcagatg caggagcgct
     2521 gaaatatctg gtgcgattca gacaatccgg aacaccagca tggaccgctg taccgtcagg
     2581 aatcaacaat aaaaacattt caggtctgat gccatccact gcttatgagt tccaggtgcg
     2641 cagtttctgc ggaccggata ataaaagcgg ctggagtact tcttccgtat ttaccacttc
     2701 accacttcgc actgctgata cctgcgaagc aagttttaca tacagcattg tcaatacggg
     2761 gttgtgtgag cttaccaata ctactgcagg tgccgcttca tgtggtgctc cgaccatatt
     2821 ttactgggag ttctgtggag atgaccctac caacgtagag aacccatact acgaattcac
     2881 agacactttt tgtatctgcc tgacggttac attcacaagt ggcttcattt ccaccacgtg
     2941 ctctgacacc ttgctgtaca cagcattgga agacattggc atatcagacg gactgttctt
     3001 gtatccaaac ccagtggccg atctcctgtt cgtcagtaca tctgatgcag atatgcgatc
     3061 tgcatggaag atcagcgatc tgtccggcag gcagatggac aacggcaaac tacatcacaa
     3121 tattcccatt gatgttcatg cacttcctgc cggtatgtat gtctttgagc tggacagtgg
     3181 aagtcgccgg gtgtttgtga aagagtgatc tgctgcagta gccttacctt tacagttcca
     3241 tgtccaaaca cctcctcatc atctgcgggc ccacagcctc cggaaagaca accctcgcca
     3301 ttgcgctggc gaagcatttc ggtacggaga tattatcggc cgacagcaga caggtctata
     3361 aagaactgaa gatcggcgtg gccagaccaa ctgaagagga actggccgct gttccccacc
     3421 actttatcgc caccaactcc atccacgagc ccctcagcgc aggacggtat gaacaggagg
     3481 ccctggcttt attggaaaag cttttccagc aacacgatgt ggtggttatg gccggtggca
     3541 gcggactatt tatcaaagcg gtaacagaag gcctggacaa cctgcctgcg agccacgaca
     3601 tgaaagtgca gttgcacgaa gaactggacg aaaaaggcct ggaatggctg caacaggaag
     3661 tggaaaaact ggacccggaa tactatgctt cggccgacca gcagaacccc cgcaggctgc
     3721 tacgggcgtt ggaggtgatc cgcctgagcg gacagacata ctcttccctg cggacaggca
     3781 aggccaagga aaggcccttc cgttctcatc tgtatctgcc ggaatgggac cgcgatgtct
     3841 tatatgaccg catcaacaaa cgggtggacc agatgatggc cgaaggccag gaagaagaag
     3901 cccgttctct cctgccccat cgccacttaa gtactttaca tacagtaggt tacagcgaac
     3961 tgttcgacca ctttgacggg ctgtatgacc tggagaccgc cgtggccctc atcgcccagc
     4021 atacccgaca ttatgccaag cgtcaactca cctggttcag gaatgaagcg ccggccacac
     4081 cggtgtccca cacaacgcag gaggctctgg aacagatcat taccggctat gaaaaaatgt
     4141 aggatcatgc atgcagaagt ggtgcacgta tcatgatacg gatcctgctg atgagtgata
     4201 cccatggtta catggatgag cgcatactgg aacatgcagc agaggctgat gaggtctggc
     4261 atgcgggtga tatcggtccg ctggaaagta tcagagagat cagacgcatc aaaccggtgc
     4321 gggctgtgtt cggcaatata gatggcagag aagtacgcca acaggcaccg ctggacaacc
     4381 ggtttgaact ggacggactg gatgtctgga tgacgcatat cggtggatat cccggtcgct
     4441 atgaacccag ggtcagggag atcctgaaag tgaagccacc cggcctgttc atttgcgggc
     4501 acagtcatat actcaaggtg cagttcgacc agaaataccg gatgcttacc atgaatcccg
     4561 gtgctgcggg tatgaatgga tttcataagg tacgcaccat gctacgattt gctattgatc
     4621 agggggaggt gaaggatctg gcggtcattg agctggggcc gcgaggacgg acaatttagt
     4681 tggcagttgg ctcccgatag ct
//
LOCUS       DAOMAX010000212         4695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3170_length_4695_cov_3.812069, whole genome shotgun sequence.
ACCESSION   DAOMAX010000212 DAOMAX010000000
VERSION     DAOMAX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3170_length_4695_cov_3.812069"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..2796
                     /locus_tag="PK742_10160"
     CDS             388..2796
                     /locus_tag="PK742_10160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU76995.1"
                     /translation="MTLQGASASTFARKNSLRNLLILIASLSAINTHAQTTEINGRIL
                     DATNHDPLTMATVVLQGTNYATVTDDEGHFQLSVPPGLYNVEVSYTGYETWVEQEVIT
                     STARPVLLNIELVESIEELIAVQIESEGFKRTDETPLSIRNFSYAEAQRMPGAVLDLS
                     KAIQSYPGVLPKSTFGYNIVFRGGAPSENRYFLDGIPIPSITHFSVQGSSGGPNGLVN
                     IDFIRGVDIYTAGFPASRGNALSGIMEINQREGRIDRFGARITLGATDYGATIEGPMG
                     QRSSYILSARHSFSQYLLKAFNVPVLPTYSDVQYKQTIHFDDKNELNIVALAAYDVYR
                     LNTDAPESDALLYNVGYIPEGDQNQYTIGLNYRHYLEHSWYSVMYSHTGLSNRADKFR
                     NNSGLEADRLLRYDGQNNSDHFRLEQKIFKGKHTFGYGMEAAYQQDVYDVFGFHISQI
                     SIDTINGVQEIGVAQAGVYGTYDLHLAGDRFVLHTGLRTDMAGYSAGTSNPLKQLSPR
                     IAMQYRATDKLSINASSGIYYQLPNSVLLAFTDSTLRDGISWIRSPQVAIGADYRNAD
                     HYRVSLEVFYKGYDNYPFLLEDSIAFANAIADYVVVGDQPAASIGKGRAYGLEWFIQQ
                     KLKRDYWWMVSYTYSVSEFQDKNGEYRPAVWDSRHFISLTAGKTWKSGWQVGGRWRYS
                     SGTPYTPYDSVASSLISNWEVANRGIFDYNQLNTERLAAFHMLDLRVDKRWNFERWQL
                     NIFMDIQNAYGSKVQLLPYLTTVRDVNWDPVVDPDDPTRYELQQINSDTGRRLYTIGM
                     VVDL"
     gene            complement(2909..3868)
                     /locus_tag="PK742_10165"
     CDS             complement(2909..3868)
                     /locus_tag="PK742_10165"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HQU76996.1"
                     /translation="MTRKQFIKGSAMIGMTLAVPGFVKASGLIGSGCERKVYRIVTAD
                     RAKVGTLPILRAFAGNHTDFVSPYVMLDEFGPVNLNPGADPLRVDAHPHAGVIPTTYF
                     LEGSGHHKDSLNYDFQITKGEFMMFSSGKGAIHMEETGQLLYDNGGNYHGFQIWLNMP
                     SVYKWTDPTTSVYRSSEMDEVHTDTFDAKVVLGELFGAKSNISLLSPAFYYHIHLKPG
                     ARMTIPTDPTHNAFLYLINGEMELESQRSLKANQMALYYRDASDIELFSETGAEFLAL
                     GGQPLNETVYAYGPFVMNNEDEIRRCIANYQSGKMGDPDVVNH"
     gene            complement(3921..4289)
                     /locus_tag="PK742_10170"
     CDS             complement(3921..4289)
                     /locus_tag="PK742_10170"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HQU76997.1"
                     /translation="MRYGSHPLNLALRFFLELTAWFAPGWWAWHRFGWWAGIILGVAL
                     MAIWVVFAVRGDESRSGKTVVQTPGRIRLILEVCMFLFGVWTVRASGYPDLAWILLAV
                     VILHHLFSADRLEWLWFWQP"
     gene            complement(4354..>4695)
                     /locus_tag="PK742_10175"
     CDS             complement(4354..>4695)
                     /locus_tag="PK742_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQU76998.1"
                     /translation="TPAVTRPAVNVAEFDKHFQLEFAAPGFDKSDFRVEVEKDQLIVS
                     VAKEENTEEKNDQYTRREFRYGNFRRMFTLPDSVDAGKVEAHYENGILRVFLPKKETE
                     IAAAKRMIEIK"
BASE COUNT         1212 a         1207 c         1160 g         1116 t
ORIGIN      
        1 ctgagccgac tgcccagggg ctcctaactc cattttaatg cctctccggg cttgacccgg
       61 agtctccttc tctatggttt gtaaaaaggc ccgccggcag gcaggaagtc tcattttttt
      121 aaagtacgat caaacaacag ctagcctgaa ggccttatcc ctacaagaaa gagggagact
      181 ccgggtcaag cccggagaaa caccgttaat gaaatggtta ataaacacga aaagtatcat
      241 gcgtctccgg cccccgagcc gcctgcccgc cgtcaggcag ggagtccccc tcactatggt
      301 ttgtacaaag cctgcctgat ctttaatcca gatcaggtat ttcgtaattc tgttaaactt
      361 ttctcaccac accctcctga caattccatg acattgcagg gtgcatctgc atctaccttc
      421 gcacgcaaaa attcattgcg gaatctttta attctaatcg cttcattatc agcgattaat
      481 acccacgccc aaaccacaga gatcaacggt cgcatcctgg atgccaccaa ccacgacccg
      541 ctcactatgg ccacagtggt tcttcagggg acaaactatg ccacggtaac ggatgatgaa
      601 ggtcattttc aattatcagt accgcccgga ctgtacaatg tagaagtttc ctatacaggc
      661 tatgagacct gggtggaaca ggaggtcatc acctccacag ccaggccggt actgctgaat
      721 attgaattgg tggaaagtat tgaagaactc attgccgtgc agatagaaag tgaaggcttc
      781 aagcggaccg acgaaacacc gttatccatc aggaatttct cttatgcaga agcccagcgg
      841 atgcccggtg cagtgctgga tctctcgaaa gccattcaga gttacccggg agtattgccc
      901 aaaagtacct tcggatacaa cattgttttt cgcggtggag ctccatcaga gaaccggtat
      961 ttcctggacg gaattcccat cccatccatc acccacttca gcgtgcaggg cagcagcggc
     1021 ggacctaacg gactggtcaa tatcgatttc attcgtgggg ttgatatcta tactgccggt
     1081 tttccagcct cgagaggaaa tgccctgagc ggtatcatgg agatcaatca acgggaagga
     1141 cgtatagatc ggtttggtgc ccgcatcacc ctgggtgcaa cagattatgg tgctacgata
     1201 gaaggtccga tggggcagcg ttcatcatac atcctgtctg caagacattc cttcagccag
     1261 tacctgctca aagcattcaa tgtaccggta ttgcctacct actctgatgt acaatataaa
     1321 cagaccatac acttcgacga caagaacgaa ctgaatattg tggcactggc tgcatacgat
     1381 gtttaccggt taaacacgga tgcgcccgaa agcgatgcac tgctctacaa cgttggatat
     1441 attccggagg gagatcaaaa ccagtacacc atcggactga actatcgcca ctatctggaa
     1501 cattcatggt attcggtcat gtacagtcat acaggactga gcaaccgggc ggataaattc
     1561 aggaacaaca gcggactgga agcagatcgt ttgttgcgtt atgacggaca gaacaacagc
     1621 gatcacttcc ggctggaaca aaagatcttc aaggggaaac atactttcgg atacggaatg
     1681 gaagccgcat accagcagga tgtatatgat gtattcggat ttcatatcag ccagataagc
     1741 attgatacca tcaatggtgt gcaggagatc ggagtggcac aagccggagt atatggtacg
     1801 tacgacctgc atctggcagg cgatcgattt gtattgcata ccggtcttcg cacagatatg
     1861 gcaggttaca gcgcaggaac cagcaacccg ctgaagcaat tgtcgcccag gatcgccatg
     1921 cagtacagag ccacagataa gctcagtatc aatgcatctt caggcatcta ttaccaattg
     1981 cctaacagtg tgctgctggc atttactgac agtaccctgc gggatggtat cagctggatc
     2041 agaagtccgc aggtggccat tggtgccgac tatcgcaatg ccgatcacta tcgggtgagc
     2101 ttagaagttt tttataaagg gtatgacaac tatcccttcc tgctggaaga ttccattgca
     2161 tttgccaacg ccattgcaga ttatgtggtg gtgggcgatc agcctgctgc ttccatcgga
     2221 aagggaaggg cctacggact ggaatggttc atacaacaaa aactcaagcg tgattactgg
     2281 tggatggtat cctataccta ttcggtctct gaatttcagg ataagaacgg cgagtatcgt
     2341 cccgccgtct gggacagtcg ccacttcatc agcctgaccg caggcaagac ctggaagagc
     2401 ggctggcagg tgggaggcag atggcgctac agcagtggca caccttatac tccctatgac
     2461 tctgtggctt catccctcat ctccaactgg gaggtggcca atcgcggaat attcgattat
     2521 aatcagttaa acacggaaag actggcagct tttcacatgc tggatcttcg ggtggataag
     2581 cgctggaatt ttgaacgctg gcaattgaat atattcatgg acatacagaa tgcctatgga
     2641 tccaaagtgc agttgctgcc ttacctcact acggtgcgtg atgtaaactg ggatcccgtg
     2701 gtggatccgg atgatcccac ccgttatgag ctgcagcaga tcaacagtga taccggtcgg
     2761 cgactctaca ccatcggaat ggtggtggat ctgtaggaga aaataatagc ggcaattcag
     2821 ttaagctatt accggcaaca acctgcagtt ttagccttcc ccttgtgcgg atatctcagg
     2881 tcttcgtaag acccagagtt ctttgtgatc agtgattcac tacgtccgga tcgcccattt
     2941 tcccggactg ataattggca atgcaacgcc tgatctcgtc ttcgttattc ataacaaaag
     3001 gaccgtaggc ataaaccgtt tcgttgagcg gttgtccacc cagtgccagg aattctgcac
     3061 ctgtttcaga gaagagttca atatcactgg cgtcgcggta gtacaaagcc atctggttgg
     3121 ccttcagaga ccgctgactt tccagttcca tttcaccgtt gatgagatag agaaaggcat
     3181 tgtgggtggg gtcggtcgga atggtcatgc gtgcacccgg ttttagatgg atgtggtagt
     3241 agaacgccgg cgataagagg ctgatattgc ttttggcgcc gaataattca cccagaacga
     3301 ccttggcatc aaaggtatcc gtatgcactt catccatttc tgacgaacga tatacactgg
     3361 tggtagggtc ggtccattta tagaccgacg gcatattcag ccagatctga aagccatggt
     3421 agttgcctcc gttgtcatac agcaactgac cggtctcttc catgtgaata gcacccttgc
     3481 cgctgctgaa catcatgaat tctcccttgg tgatctggaa gtcatagttc agactgtctt
     3541 tgtggtgacc gctgccttcc agaaagtagg tggtgggtat cacaccggca tgtggatgtg
     3601 cgtccacccg caaagggtct gcacccggat tcaggtttac aggcccgaat tcgtcgagca
     3661 tgacataagg agaaacaaaa tccgtatggt taccggcaaa ggctctcaga atgggtaagg
     3721 tacccacctt ggctctgtcg gccgtaacaa tgcggtacac ttttctttca caacctgaac
     3781 cgatcagtcc cgaagcttta acaaagcctg gaacagccag cgtcatgccg atcatggcag
     3841 agcctttgat gaattgcttt ctggtcataa tgcctgattg tcttcctaaa catacaacac
     3901 agctccgaag ttcagaatca tcatggctgc caaaaccaaa gccattccag gcggtcggca
     3961 gaaaacaggt gatgaagtat gacgactgcc agcaggatcc aggccagatc cggatatccg
     4021 gaagcccgga cagtccagac gccaaacagg aacatgcaga cctccaggat cagcctgatc
     4081 ctcccgggag tctgcacaac ggtctttcca ctccgacttt catcgccgcg aacagcaaat
     4141 acaacccata tagccatcag ggcgacaccc agaataatgc ctgcccacca cccgaagcga
     4201 tgccaggccc accagcccgg tgcgaaccat gcggtgagct ccagaaaaaa acgcagggcg
     4261 agattcagcg gatgactacc atatcgcatg acctaaagaa ataaaaaaag cgccgtcttt
     4321 caacggcgct ccacacctta aacctacact tgcttatttg atctcgatca tgcgcttggc
     4381 agcggcgatc tctgtttctt tcttcggcag gaaaacacgc agaatgccat tctcatagtg
     4441 ggcttctacc ttgccggcat ccacactgtc aggcagggtg aacatgcggc ggaagtttcc
     4501 gtaacggaat tcgcggcggg tgtactggtc gttcttctct tctgtgttct cttctttggc
     4561 tacgctgacg atgagctggt ctttttctac ttccacgcgg aagtcagatt tgtcgaatcc
     4621 cggtgcagca aattccaact ggaagtgctt gtcaaactca gccacattca cggccggacg
     4681 ggtgacagcc ggggt
//
LOCUS       DAOMAX010000213         4655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3222_length_4655_cov_4.098478, whole genome shotgun sequence.
ACCESSION   DAOMAX010000213 DAOMAX010000000
VERSION     DAOMAX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3222_length_4655_cov_4.098478"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PK742_10180"
     CDS             <1..717
                     /locus_tag="PK742_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HQU76999.1"
                     /translation="WVIIASVLLVMMESVAGLETDHGRLFHDLEWGFTILFSIEYLLR
                     IYSHPKPLTYVFSFWGLIDLLSILPAYLSLVLAGTHFLTVIRILRLARVFRILKLGRY
                     FSESQTLGKALYASSYKITVFLVAVLLVVITLGSVMYVVEGGNNGFSSIPQSIYWAII
                     TITTVGYGDIVPVTALGKFFASLIMIIGYAIIAVPTGIITAEITKAQMGKPACPHCGK
                     ELPDGAIAFCPWCGEPVKKA"
     gene            774..4181
                     /locus_tag="PK742_10185"
     CDS             774..4181
                     /locus_tag="PK742_10185"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQU77000.1"
                     /translation="MFAYRKLNNWMGWLIWLIATMVYLLTMEAGASWWDCGEFIAATY
                     KLQVVHPPGAPIFLMVGRIFSLFASSPEQVPVMTNIFSALSTSFSILFCFWIITRLAK
                     KMIAGEEEEPDSLQKLLIIGSGVVGALTCTFLDSVWFSAVESEVYALATFFFALIFWA
                     MIKWEEMADTPKGDRWIIFIFLTLGLSMGVHLLSLLAIPAMGMIYYFKNYTYTRRGLW
                     LAILINLGILVFVLFGVLDKFIAIAAAFDRALAGIGMGVGIAVFTLILAGLVFYGVRY
                     AIQKNKRTLYIGLMSFTMMMIGLSSYAMVLVRAHAEPPINMNGINDVHSFLSYLKREQ
                     YGSRDLVYGTYWTAQPLNVEYGKEKWGRKPGGKEYVPIGKEFKLIYDLPDAQLAAYGM
                     TPQQIQILRGRNKKVIFPRMGSPEGRHAGLYYNFANVPDDQQNTYIPSYGTNLNFFFT
                     YQMGYMYWRYFMWNFSGRQNDSQGFYAEAMKDGNWITGIEGIDKLKNPNIDRLPQSQR
                     DLKSRNVFYMLPFIIGLLGMVYQLKKDWKGFLVVFMFFFFMGIMNLVNSNQPPVEPRE
                     RDYALVGAFFAFAIWVGFGVLALFDMAKSNKNKLTEYLLYMGILMVSFFITGLTVYDL
                     DSFIALLFYTAIVTGIMYLLVLGARMVTGNWTGSAVVALLLCLSAPLLMGFQGWDDHD
                     RSNRTMARDFARNYLESCPPNAILFTQGDNDTYPLWYAQEVEGIRTDVRIINMSLLGV
                     DWYINHLRHATNDAGVVDLLLDQDKVFGDKRNSVRYNDKSKFRDRELDLKDMVLFMGS
                     DKAEALDADGDNYLPSLHARVTVDSAAVADKDLVPEHLKGNITTIHFDINNKTLLKND
                     LMVLDIIAGNINKRPICFAITVSPDAYMGAEKYFMQTGMVYRLTPVEIDGRGMGREVG
                     EEVTYDLLINHAELFTYGGIERHLNMNLDPSSLGSAMTAKYVMYQQLAANLVQDKLDK
                     EAQAALMRADSSRTDMLEVAEGLEAEAAQSQEMAITVLNKMMEQFPEEVLPYDYNMVN
                     AASYYQILGETEQSLKIVRSLSETVLDDLAYFYYLYNKPADGYVCRSQYAADQRDAER
                     CVGSLINIARKDGDSELSDSIKKRWEMLQAEFNIRTNAPQQAAPPAR"
     gene            4147..>4655
                     /locus_tag="PK742_10190"
     CDS             4147..>4655
                     /locus_tag="PK742_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HQU77001.1"
                     /translation="MRPNRQPHRHAKRRAHDNLVFGRQPVAELLQGDKEVDSILIQTG
                     AQGDVLKQIRQLAVMKGVPVKSVPVEKLNRLTGGNHQGVIAFTAAISYVSIADLLPLI
                     IESGEVPLIIVLDGITDVGNFGAIARTAWAAGAHALVIAGTGAAPVNADAMKASAGAL
                     QEIPVCREA"
BASE COUNT         1157 a         1101 c         1188 g         1209 t
ORIGIN      
        1 tgggtcatca tcgccagtgt tctcctggtg atgatggaaa gcgtggccgg gctggaaacc
       61 gatcatggca ggttgttcca cgacctggaa tggggcttca cgatcctgtt ctccatagaa
      121 tacctgctgc gcatttacag ccatcccaaa ccactcacct atgtattttc tttctggggt
      181 ttgatcgacc tgttgtccat attgccggct tacctcagtc tggtgctggc aggcacccat
      241 ttcctgaccg tcatccgaat cctgcgcctt gcaagggttt tcaggatcct taaactaggg
      301 cgatactttt cagaatcaca gacattggga aaggcattgt atgccagctc ttataagatc
      361 actgtatttc tggtggctgt attgctggtg gttatcacac tgggttctgt catgtatgtg
      421 gtggaaggcg gcaacaacgg attcagcagc atcccacaga gtatttactg ggctatcatc
      481 accatcacca ctgttggcta tggcgacatt gttcctgtaa ctgccctggg taaattcttt
      541 gcttctctca ttatgatcat aggctatgcc atcatagcgg ttcctaccgg tatcatcaca
      601 gcagagatca ctaaagcaca aatgggtaaa ccggcctgcc cacattgcgg gaaggagctt
      661 ccggatggtg ctattgcgtt ttgcccctgg tgcggagagc ctgttaagaa agcctaaaaa
      721 cctgcattcc taccgcctga atcagttatc tttaggcctt caaaatcaag cgcatgtttg
      781 catacaggaa gttgaacaac tggatgggat ggctcatatg gctgatcgcc accatggtat
      841 atctcttgac catggaagcc ggggccagct ggtgggattg tggtgagttc atcgcagcca
      901 cctataaact gcaagtagtt cacccgcccg gagcacctat tttcctgatg gtgggccgta
      961 tcttcagcct gtttgcctcg tcgcctgaac aggtgccggt catgacgaat attttctctg
     1021 cgctgagtac ttccttctct attctgttct gcttctggat cattacccgc ctggctaaaa
     1081 agatgatagc cggagaagaa gaagaaccgg atagcctgca aaaactgctc atcattggat
     1141 ccggtgtggt aggtgcgctg acctgtactt tcctggattc tgtctggttc agcgcagtgg
     1201 aaagtgaagt atatgctttg gccaccttct tctttgccct cattttctgg gcgatgatca
     1261 aatgggaaga aatggccgat actcctaaag gagaccgctg gatcattttc attttcctga
     1321 cacttggtct gtccatgggt gtgcatctgc tcagcttgct ggccattccg gctatgggaa
     1381 tgatctacta ttttaaaaat tacacctaca cccgtcgtgg tctctggctc gctatcctga
     1441 tcaacctggg catactggta tttgtcctgt ttggagtgct ggacaagttc attgccattg
     1501 ccgctgcttt tgacagagca ttggcaggca taggtatggg tgttggcata gctgttttca
     1561 ctttgatact ggcaggactg gtattctatg gtgtgcggta tgctattcag aaaaataaaa
     1621 gaacacttta catagggctg atgtcattca ccatgatgat gatcggtctg tcttcgtatg
     1681 ccatggtgtt ggtgcgggct catgcagaac cacctatcaa catgaatggt atcaatgatg
     1741 tacacagttt tctctcctat ctgaaacgcg aacagtacgg cagcagagat ctggtctatg
     1801 gtacctattg gaccgctcag ccactcaatg tggagtatgg taaggaaaaa tggggacgta
     1861 aacccggcgg aaaggaatac gttcccattg gcaaggaatt taaactcatt tacgacctgc
     1921 ctgatgcaca gcttgcggcg tacggcatga ctcctcagca aatacagatc ctgcgaggaa
     1981 gaaataaaaa ggtcatcttc ccgcgaatgg gaagccccga aggcagacat gccggcttgt
     2041 attacaactt tgccaatgtg cctgatgatc agcaaaacac ctacatccca tcatacggca
     2101 ccaacctgaa cttcttcttc acctaccaga tggggtacat gtactggcgt tatttcatgt
     2161 ggaacttcag cggacggcag aatgactctc agggctttta tgcagaagcc atgaaagatg
     2221 gcaactggat aaccgggatt gaagggatcg ataaacttaa gaaccccaat atagaccgcc
     2281 tgccacaaag ccagcgagac ctgaaatcca ggaatgtatt ttatatgctc cctttcatta
     2341 tcggtctgtt aggaatggtg taccaactga aaaaagactg gaaaggattt ctcgtagtgt
     2401 tcatgttctt cttctttatg ggcatcatga acctggtgaa cagcaaccag cctcctgtag
     2461 aaccacgcga acgtgactat gccctggtag gtgccttctt tgcctttgcc atttgggtag
     2521 gctttggtgt tctggctttg tttgatatgg cgaagagcaa taagaacaag ctgaccgaat
     2581 acctcctgta tatgggcatt ctgatggtga gcttctttat taccggattg accgtatatg
     2641 atctggacag cttcattgca ctactgttct acactgccat tgtaacgggc atcatgtatc
     2701 tgttggtgct gggagcacgc atggtaaccg gcaactggac cggatctgcg gtggtggctt
     2761 tattgctttg cttatcggca cctttgctaa tgggttttca gggatgggat gaccatgacc
     2821 gcagcaaccg caccatggca agagacttcg cccggaatta tctggaaagc tgtccgccca
     2881 atgccatcct gttcacccag ggtgataatg atacctatcc tttgtggtat gcacaggagg
     2941 tggaaggtat ccgcacggat gtacgcatca tcaacatgtc attgctgggt gttgactggt
     3001 atatcaatca tctgcggcac gccaccaatg atgcgggtgt ggttgacctg ttgctggatc
     3061 aggataaggt attcggagat aagcgcaact ccgtcagata caatgacaag agcaaattcc
     3121 gtgaccgtga actggacctg aaagacatgg tactgttcat gggatcagac aaggcagaag
     3181 cccttgatgc cgacggagac aactacctgc cttccctcca cgcaagggta acggttgatt
     3241 ctgccgctgt tgcagataaa gatctggttc cggaacatct caaaggtaat atcactacca
     3301 ttcactttga tatcaacaac aagaccctgc tgaaaaacga tctcatggtg cttgatatca
     3361 ttgcaggaaa catcaataaa cgccccatat gttttgccat cactgtaagt cctgatgcct
     3421 atatgggtgc agagaaatat ttcatgcaaa caggcatggt gtacagactt accccggttg
     3481 aaattgatgg tcggggcatg ggccgcgagg tgggtgaaga agtaacatat gatcttctga
     3541 tcaaccatgc tgaactgttc acctatggtg gtattgaacg ccacctgaat atgaatctgg
     3601 atccaagttc tctcggttct gctatgaccg ccaagtacgt gatgtaccag cagctggctg
     3661 ccaatcttgt tcaggataaa ctggacaagg aagctcaggc tgctctgatg cgtgctgact
     3721 ccagccgaac agacatgctg gaagtagctg agggcctgga agcagaagct gcacagagtc
     3781 aggaaatggc cattacggtt ctgaacaaga tgatggaaca gtttccggaa gaagtgttgc
     3841 cgtatgacta taacatggta aacgctgcca gttattatca gatccttggc gagacagaac
     3901 agtctctgaa gatagttagg agcctgtctg aaaccgtcct ggatgacctg gcttatttct
     3961 actacctgta taacaagcct gcagatggct atgtatgccg gtcgcagtat gccgcagatc
     4021 aacgtgatgc tgaacgctgt gtgggcagtc tgatcaatat tgccagaaaa gacggtgatt
     4081 ctgagctttc tgacagtatc aaaaagcgct gggaaatgct gcaggcagaa ttcaatatca
     4141 gaaccaatgc gccccaacag gcagccccac cggcacgcta agcgcagggc acacgataac
     4201 ctggtattcg gcaggcagcc ggtagcagaa ttgttgcagg gcgataagga agtggacagc
     4261 atcctgatcc agacaggagc ccagggtgat gtactgaaac agatccgtca actggctgtc
     4321 atgaaaggcg taccggtcaa atctgtcccg gtagagaaac tcaacagact gacaggtggc
     4381 aaccaccagg gtgttatagc gtttactgct gcgatctctt atgtttccat tgcagatctc
     4441 ctgcccctga tcattgaatc aggtgaggtt cctcttatta tcgtgctgga cgggataacc
     4501 gatgttggaa actttggagc cattgccagg accgcctggg ccgccggagc gcatgcgctg
     4561 gtcatagcag gaacaggcgc tgccccggtc aatgcagatg ccatgaaagc aagtgcaggg
     4621 gctttgcagg aaattccggt atgcagggaa gccat
//
LOCUS       DAOMAX010000214         4596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3297_length_4596_cov_4.159216, whole genome shotgun sequence.
ACCESSION   DAOMAX010000214 DAOMAX010000000
VERSION     DAOMAX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3297_length_4596_cov_4.159216"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..843)
                     /locus_tag="PK742_10195"
     CDS             complement(88..843)
                     /locus_tag="PK742_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HQU77002.1"
                     /translation="MNLITLTTDLGLRDHYVAVLKGKILRHFEDLQFMDISHEVEPFN
                     VQQGAHLLRAGWEAFPEGTVHVVAVNMQHTNPLRCIFFAMGGHYFAGPDNGIFPLAIS
                     NMPRDVFTPAASGNNMDDLALVAAGLAEGHPLNEIGTLTGNVFMLQNLQSPFSDSMIR
                     TRVNYIDHFGNVVLAISTEEFTRVLGDRNFDIEFGKREHISQISASYADVPEGEKCCI
                     LNDSGYLEIAINKGNAHELLGLKINDTVLIEFT"
     gene            900..1874
                     /locus_tag="PK742_10200"
     CDS             900..1874
                     /locus_tag="PK742_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQU77003.1"
                     /translation="MIEVLIPLEGVRAIDFYGVHNARLGIIKKSFPYLNIAARGNELR
                     VRGSEKDIQEFQDKIGKVLHHLEKYGQISDSNLQSLLFGVENNDLPDVQAIPEDVIVH
                     GPNGHLVKARTPNQKKMLAMADHDDIIFAIGPAGTGKTYTAVALAVRALKDKLVKRII
                     LTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALDDMIPIEKLNLFMANRVIEIAPLA
                     FMRGRTLDHAFIILDEAQNATEAQLKMFLTRIGPSARCIITGDLTQIDLPRNQRSGLF
                     NALRLLKDIDGIGTVFLDEKDVVRHRLVKQIIKAYDKDRLEHEAREED"
     gene            2073..2507
                     /locus_tag="PK742_10205"
     CDS             2073..2507
                     /locus_tag="PK742_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77004.1"
                     /translation="MDSTSYDLVLQLQQTCAKLETLIVSADQPWTDHGYVCVEAMISG
                     QAFTIPVDDEYDDPITANKAMQWHMILRTVADYEDSNDFLTWCNELGMDSTSLEVLAM
                     YRHLNDIVPGLRRVAGEVQPLSHFDQQMNTGAMQSLRNRVGY"
     gene            2531..3577
                     /gene="aroB"
                     /locus_tag="PK742_10210"
     CDS             2531..3577
                     /gene="aroB"
                     /locus_tag="PK742_10210"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQU77005.1"
                     /translation="MSPTADLSYVQLLSASPDWQSLAQADRYSSIFILTDNNTQEHCL
                     PLLQDAFPAAIHLHIPAGEKEKNIETCSYLWQEMLMHNADRQALLINLGGGVIGDMGG
                     FVAGTYKRGINFIQIPTTLLSMVDASVGGKLGIDHHGLKNVVGLFRQPQAVLIYPEFL
                     KTLPERELRSGYAEVLKHGLIADAAYWNETAAGLPDADKWSDIIRRSVMIKQEVVEDD
                     PLENGRRKILNFGHTVGHALETWSLLHDEDPLLHGEAIARGMVIEAALSRELTGLSTE
                     EEHHITTAMMRFFPEKKSAANATELLHLMMNDKKNTKGEIRFSLLGSIGHCRWDIPVP
                     ADVLLSTLERFGHA"
     gene            3570..>4596
                     /locus_tag="PK742_10215"
     CDS             3570..>4596
                     /locus_tag="PK742_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77006.1"
                     /translation="MPDLIVDGHNASVQGSIELEGSKSLSNRLLIMQAMADEKLELRG
                     LSPSDDTEALQRILKSKSSAGNVGAAGTTMRFLTAFYAAHPGDQVLTGSARMLQRPIG
                     ILVDALRALGAVINYAGEEGYPPLHIRGSKLRGGKLSIRADVSSQYISALLMVAPEME
                     NGLELELTGKVGSFPYIRMTLELMSMLGIRYSIDRQVIRVEPGKYHGGIVQVEGDWSA
                     ASYYFGLCALADDPQVDIHGLFSRSLQGDSVLPAIYAQLGVVTAWDGDVCRLTKTGNT
                     VNYFEYDFSDCPDLAQTVVVTCAALGIPGSFSGLESLRIKETDRTAALAAELGKFGVQ
                     FTQDGDSW"
BASE COUNT         1164 a         1137 c         1214 g         1081 t
ORIGIN      
        1 tactgattcg cggctgagct tctgcaaaaa cagccctgaa tgtttccgtc tcatcgtggc
       61 ggaaactcat tttgacaatt cgtctgatca tgtaaattct atgagtacag tgtcgttgat
      121 cttcagtccg agcaattcat gcgcattgcc tttattgata gcaatttcca ggtagccgct
      181 gtcgttgaga atacagcatt tctcgccttc cggcacatca gcataagagg cggagatctg
      241 gctgatatgt tcccgctttc cgaattcgat atcgaaattc ctgtcgccaa gtaccctggt
      301 gaattcttct gtgctgatgg ccagcaccac atttccgaaa tgatcgatgt agttgacacg
      361 ggtacggatc atgctgtcac tgaaaggaga ctgcagattc tgcagcatga agacgttgcc
      421 ggtaagcgtt ccgatctcgt tgagcggatg tccttccgca agacccgcag ccacaagagc
      481 caggtcgtcc atattgttgc cgctggcagc cggagtgaac acatcacggg gcatgttcga
      541 aatagcgagc ggaaaaatgc cgttatccgg accggcaaaa tagtgcccgc ccatagcaaa
      601 gaagatgcag cgaagcggat tggtgtgctg catgttcacc gctaccacat gcaccgttcc
      661 ttcaggaaat gcctcccatc ctgcacgaag aaggtgagcc ccctgctgca cattaaaggg
      721 ttcaacctca tggctgatgt ccatgaactg caggtcctcg aaatgccgga ggatctttcc
      781 cttcagtacc gccacatagt ggtcgcggag gcccaggtca gtggtcaggg tgatcagatt
      841 catcggctcg gcggttgcgc caaatggttt agatttgttg gcaacaaaag taaccgaatt
      901 tgatagaagt attgatcccg ctggagggtg tcagagccat tgatttttac ggtgtgcaca
      961 atgcacgact gggtatcatc aagaaatcat tcccctatct caatattgcc gcacgtggca
     1021 acgagcttcg tgttcgcgga agtgaaaagg atatccagga atttcaggat aagatcggca
     1081 aggtcttgca ccatctggaa aaatacggcc agatcagcga ctccaacctg cagagtctgt
     1141 tattcggagt ggagaacaac gacctgcccg acgtgcaggc catcccggaa gatgtgatcg
     1201 tacatggccc caacggacac ctggtaaagg cccgcacacc taaccagaag aagatgctgg
     1261 ccatggcaga tcatgatgac atcatcttcg ccatcggacc ggcaggaaca ggcaagacct
     1321 atacagctgt ggcacttgca gtacgtgcgc tgaaagataa actggtaaaa cgcatcatcc
     1381 ttaccagacc ggcagtagaa gcaggtgaga acctcggctt cctgccggga gatctgaaag
     1441 aaaagatcga tccctacctg cgacccctct acgatgcgct cgatgatatg atccccatcg
     1501 aaaaactgaa cctttttatg gccaatcggg tcatagagat cgcaccgctg gccttcatgc
     1561 gtggtcgtac cctcgatcat gccttcatca ttctggatga agcgcagaat gccaccgaag
     1621 cacagctcaa aatgttcctg acccgtatcg gtccgagtgc ccggtgcatc atcaccggcg
     1681 atctcaccca aatagacctg ccgcgaaacc agcgaagcgg actgttcaat gcacttcggt
     1741 tgctgaaaga tattgacgga attggtaccg tattcctgga cgaaaaagac gtggtcagac
     1801 accggctggt caaacagatc atcaaagcct acgataagga caggctggag catgaggcga
     1861 gggaagagga ttgaccagtt ggcggttggc agtcagcagt tggcagtctg tagcgtcctg
     1921 aatacggttg acagttggcg ctcagcagct gttttccatt ccttattccg gcttaccagt
     1981 acttgatacc tgatacctga tactttctac tatccctcca ccattgttca tttgttaatg
     2041 accacacaac atgcagtgcg taccttggcg tcatggatag tacctcgtac gatctggtgc
     2101 tgcaattgca gcagacctgc gcaaaactcg aaaccctcat tgtctctgcc gatcagccct
     2161 ggacagatca cggttatgta tgtgtggaag ctatgatcag tggtcaggca ttcaccattc
     2221 ctgtagatga tgaatatgat gacccgataa ccgccaataa ggctatgcaa tggcatatga
     2281 tcctgagaac ggttgcagat tatgaagaca gcaatgattt cctgacctgg tgcaatgagc
     2341 tgggtatgga tagcaccagc ctggaagtgc ttgccatgta cagacacctg aacgacatcg
     2401 ttccgggtct tcgcagagtg gccggtgaag tacaaccctt atcgcatttt gaccaacaaa
     2461 tgaacacagg tgccatgcaa tcgctgcgaa accgggtggg ttattgatct cgtgccgtaa
     2521 ttttgccgct atgtcgccca ctgctgatct gtcttatgtg caattgttgt ctgcctctcc
     2581 tgactggcag tcactggcgc aggcagatcg gtacagcagc attttcattc tcactgacaa
     2641 caatacccaa gagcattgtc tgcctctgtt gcaggatgct tttcctgcag ctattcatct
     2701 gcatattccg gcaggagaaa aagaaaagaa tattgaaacc tgttcttacc tgtggcagga
     2761 aatgctaatg cacaatgctg atcgccaggc gctgctcatc aacctgggcg gaggggtcat
     2821 cggagatatg ggtggctttg tggccggtac ctacaaacgg ggcatcaact tcatccagat
     2881 accaaccacc ctgttatcta tggtagatgc atcggtgggt gggaaacttg gaatagatca
     2941 ccacggactg aagaacgtgg taggattgtt tcgtcagccg caggcagtac tcatctatcc
     3001 ggaatttctg aaaacccttc cggaacggga attgcgcagt ggctatgcag aggtgctcaa
     3061 gcatggactg atagccgatg ctgcttactg gaacgaaaca gctgccggac ttccggatgc
     3121 agataagtgg tctgatatca ttcggcgttc ggtcatgatc aagcaggaag tggtggaaga
     3181 tgatccattg gaaaacggca gacgtaagat cctcaacttc gggcatacgg ttggacatgc
     3241 actggaaaca tggtcgctcc tgcacgatga agatccattg ttgcacggag aagccatcgc
     3301 ccgtggtatg gtcatagaag cagcactgag ccgggaactg acaggtttgt caaccgaaga
     3361 agaacaccac attacaacag ccatgatgcg tttcttcccc gaaaaaaaat ctgctgcaaa
     3421 tgcaactgaa ctgctgcacc tgatgatgaa tgataagaag aatacgaaag gagagatacg
     3481 tttcagtctg cttggttcga taggccattg ccgctgggat attcctgtac ctgctgatgt
     3541 gttgctctcc actctcgaaa gattcggaca tgcctgatct gatcgttgac ggacataatg
     3601 cctctgtaca gggaagtata gaactggagg gctccaaaag cctgagcaac agactactca
     3661 tcatgcaggc catggctgat gaaaagctgg aactgagagg actttctccg tcagatgaca
     3721 ccgaagctct gcaacggatt ctcaaaagca aaagctcggc cggaaatgta ggcgcagccg
     3781 gaacaaccat gagatttctg actgcgttct atgccgctca tcccggcgat caggtgctga
     3841 cgggttcggc caggatgctg cagcgaccca tcggtattct ggtagatgca cttcgtgcac
     3901 tgggagctgt catcaactat gccggagaag aaggatatcc gcctctgcat atacgcggca
     3961 gcaaacttcg tggaggaaaa ctctctatac gtgccgatgt gagttcacag tacatcagcg
     4021 cactgctcat ggttgcaccg gaaatggaaa atggcctgga gctggaattg actggcaagg
     4081 tcggttcctt tccctacatc cgtatgaccc tggagctcat gtccatgttg ggtatcagat
     4141 acagtattga cagacaggtg atcagagtgg aacccggaaa ataccatggt ggcatcgttc
     4201 aggtagaggg cgactggagt gcggcatcct actatttcgg cttgtgtgct ttggccgatg
     4261 acccgcaggt ggacattcat ggtctgttta gcagaagtct tcagggcgac agtgtcctgc
     4321 ctgctatcta tgcgcaactg ggtgtagtga ctgcctggga tggtgatgtg tgccgtctca
     4381 ctaaaaccgg aaatacggta aattattttg aatatgactt ttctgactgt ccggatctgg
     4441 cacaaacggt cgtggtaacc tgtgcggccc tgggtattcc gggaagtttc agcggactgg
     4501 aaagcctgcg catcaaggag accgatcgta cggcggcgct ggccgcggaa ctcggcaaat
     4561 ttggtgtgca attcacgcag gatggagaca gctgga
//
LOCUS       DAOMAX010000215         4580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3311_length_4580_cov_4.334144, whole genome shotgun sequence.
ACCESSION   DAOMAX010000215 DAOMAX010000000
VERSION     DAOMAX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_4580_cov_4.334144"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PK742_10220"
     CDS             complement(<1..692)
                     /locus_tag="PK742_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77007.1"
                     /translation="MGKLSMAANAASTELEAAGISLDVYGIIDEVLLDANASDPYDEI
                     YSKLDQLNRGQELLQSKLDDLISEVRWMDLSNDLRPILAYIDYNWSVFHQLIDKTYVW
                     SSSAAKASKIQNFIRSVTGTGSNSMDYNLFLLNRIIVDGSGTYGKSLLEEFKTNNAQW
                     HLPGRSLLQSLELILLDLQYVQFKGYAVLQAAQNYVERQSNPVLSSLTRSQLSRLKDM
                     IENNDWPADINE"
     gene            complement(964..2994)
                     /locus_tag="PK742_10225"
     CDS             complement(964..2994)
                     /locus_tag="PK742_10225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQU77008.1"
                     /translation="MTIRILSSVVILAALASCTSNKEVSYREAFDPSTLDSSYSPTND
                     FFRFVNAKWIAENPIPGDRSRYATFDKLNEQALNTLKDVMEKASTSNAAKGSNEQKVG
                     DFYFSGMDSASINAQGASALQPYFDMINGMSGPEDFAATSAWMNRIFTGVPFVTWVGQ
                     DDKNSAEQVLNFWQGGLGLPDRDDYFRDDAQSAENRAAYKQHIVNTLVLMGRDEAMAE
                     KEARSIYAMEEKLAGASMDRVTMRDPHATYHKMSLADLSAMCPNMDWNTFFSKLGTPS
                     MDSVLVSQPDFMTALNGMIKSVPMDDWKTFMSYHLVNNVSEYLSDDFVQENFDFYQAK
                     MRGVQEMKPRWKRVVETANFTLGEALGQEYVKLAFSPESKERMIKMVDDLRASMKVRI
                     DGLDWMSADTKAKAQEKLNSFTVKIGYPDKWRDYAKLDIDRGAYVLNVMRSMEFEAQR
                     NYDKIGQPVDKTEWGMTPQTVNAYYNPSNNEIVFPAAILQPPFFDPNADDALNYGGIG
                     AVIGHEITHGFDDQGRQYDANGNLVQWWTDGDDSLFRNKADVVVSQFGNFCPTDGQCI
                     NGNLTLGENIADFGGLTIAYYAFKMTEEGKSTEPIDGFTPDQRFFLGFGRIWASSYTD
                     QAMRQQLLTNPHSPGEYRVNGTLANMPEFYQAFGVKPGDDLYKDTPEMAKIW"
     gene            complement(3061..4455)
                     /locus_tag="PK742_10230"
     CDS             complement(3061..4455)
                     /locus_tag="PK742_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU77009.1"
                     /translation="MNIKFCGAAGTVTGSSHLLTLDDGFRILLDCGLYQGREEAYENF
                     NRHFFFKPGDIDLVILSHAHIDHSGKLPRLVKEGFQGKIYATPATRDLTAILLLDSAH
                     IQERESDFTNRKMEEEHKEEPLYNVEDVTKTMELFRMVGYDHWFHVHPDVEVLFKDSG
                     HILGSASVTLRIHREGLKTTYLGFTADIGRPERPILRDPDPMPPCDYLICESTYGNRL
                     HDEAPKEREHLLEIIQQTCVVQKGKLIIPAFSVGRTQEIVYMLDQLQTEGRLPDIPVY
                     VDSPLAVNATAIFEQHPECFDKELLRYMIRDPNPFGFEKLRYVRSVDESKSLNNKKAC
                     IIISASGMMNAGRIRHHLFNNIEHPENTILIVGYAGPGTTGGALRSRVKTIRLFGQEL
                     QVNARIEIMDSFSAHGDYQEMIHYLRSQQKDKLRKLFLVHGDDDALESFRGKLLSEGY
                     NDVHIAMLGESISL"
BASE COUNT         1099 a         1192 c         1020 g         1269 t
ORIGIN      
        1 ttttcattga tatcagcggg ccagtcgttg ttctcgatca tatccttcag cctgcttaac
       61 tgtgaccgtg tgaggctact caataccgga ttgctctgtc tttctacata attctgggca
      121 gcctgcaaaa cagcatagcc cttgaactga acatactgaa ggtccaatag gatcaattca
      181 agactttgga gcaatgacct gccaggcaga tgccattggg cgttattggt cttaaactct
      241 tctaacagag atttaccgta agttcctgag ccgtcaacga ttatcctgtt taaaaggaac
      301 aggttgtaat ccatagaatt cgacccggtt cctgtaacag atctgatgaa attctgaatc
      361 tttgatgcct ttgctgcaga actgctccat acataggtct tatcgatcaa ctggtgaaaa
      421 actgaccagt tatagtctat atatgcaaga atgggtctta gatcgttgga gagatccatc
      481 cacctgacct cactaataag atcatcaagc tttgactgta acagttcctg gccccgattc
      541 agctgatcca gtttggaata tatctcatca tatggatcgc ttgcattggc gtctaatagc
      601 acttcatcta taatgccata aacatcaaga ctgatcccgg ctgcctcgag ttcagtgctg
      661 gctgcatttg cagccatact taatttgccc atttgtgttt tttgtttaga ttctaaagat
      721 attaactaag aagtataaaa tgcaaaatgg atgcagcctg catgaccctg aatctttttg
      781 tactacaata tttacattaa gcataatgcg atatgctttt tcagctatcc ggacctgcat
      841 gccatatcag atcgtcaaat aggccttctg aattagttta ttttgaattg accatacagc
      901 atataaaaag gaaagccgcc ggacatccgg cggctttctc tatgtatcta ttttcaaagt
      961 atatcaccag atcttcgcca tctccggcgt atccttatac aggtcatcgc ccggcttcac
     1021 accaaacgcc tgatagaatt ccggcatatt ggccagggta ccattcacac gatattcacc
     1081 cgggctgtgc gggttggtca gcagctgctg acgcatagcc tgatcagtat aggagctggc
     1141 ccatatgcga ccaaaaccca ggaagaatcg ctggtctggt gtaaaaccat ctatcggttc
     1201 agttgactta ccttcttcgg tcatcttgaa tgcatagtag gcgatggtca ggccaccaaa
     1261 gtcggcgatg ttctcaccca gtgtcaggtt gccgttgata cactgaccat cggtcggaca
     1321 gaaattgccg aactggctga ccaccacatc tgctttgttg cggaagaggc tgtcgtcacc
     1381 atcggtccac cactgcacta ggtttccgtt ggcatcgtac tggcgaccct gatcgtcgaa
     1441 gccatgggtg atctcatgtc cgatcaccgc accgatacct ccgtagttca gggcatcatc
     1501 tgcattcgga tcgaagaacg gcggctgcag gatggctgcc gggaatacga tctcgttgtt
     1561 acttggattg tagtaggcgt tcaccgtttg cggggtcatt ccccactctg tcttatcaac
     1621 cggctgaccg atcttatcat agttgcgctg tgcttcaaac tccatactgc gcatcacatt
     1681 caacacatag gcaccgcgat caatgtccag cttggcataa tcacgccatt tatcaggata
     1741 accgatcttc actgtgaagc tgttcagttt ctcctgtgcc ttagccttgg tatcggcact
     1801 catccagtcg agtccgtcaa tacgaacctt catactggca cggagatcgt ccaccatttt
     1861 gatcatacgc tctttgcttt ccggagaaaa tgcaagtttc acatactcct ggcccagcgc
     1921 ttcacccagc gtgaagttgg cagtttccac cactcgcttc catcttggtt tcatttcctg
     1981 tacaccccgc atttttgcct ggtagaaatc aaaattttcc tgaacgaaat cgtcgctcag
     2041 gtattctgac acattattta caaggtgata gctcatgaat gttttccagt catccatcgg
     2101 aacagacttg atcataccgt tcagagcggt catgaaatca ggctgagata ccagcacact
     2161 atccatggaa ggggtaccca gtttgctgaa gaaagtgttc cagtccatgt tcgggcacat
     2221 cgcagacaga tctgccaggc tcattttatg ataggtggcg tgtggatcac gcatggtcac
     2281 acggtccatg gaagcacctg caagcttttc ttccatcgca tagatggacc tggcttcttt
     2341 ttctgccatg gcttcatccc tgcccatcag caccagcgta ttgacgatat gctgtttgta
     2401 ggccgcacga ttctcagcac tctgcgcatc atctctgaaa taatcatcac ggtcgggaag
     2461 tcccagtccg ccctgccaga aattcagcac ctgctcagca ctgttcttgt catcctgacc
     2521 tacccaggtc acgaagggta ctccggtaaa tatccggttc atccaggcag aagtggccgc
     2581 aaaatcttcc ggaccgctca tgccgttgat catatcaaaa tacggttgca aggcacttgc
     2641 cccctgcgca ttgatagagg ctgagtccat tccgctgaaa tagaaatcac ccaccttctg
     2701 ttcattgctg cctttggccg cattggaagt ggatgctttt tccatgacat ctttgagtgt
     2761 attcaacgcc tgctcattca gtttgtcgaa agtagcatag cggcttctgt cgccagggat
     2821 cggattctct gcgatccact tggcgttcac aaaacgaaag aagtcattcg tcgggctgta
     2881 gctcgagtcg agggtggacg gatcaaaggc ctcgcgatag gatacttcct tgttggatgt
     2941 acaggaagcc agcgcagcca ggatcaccac cgatgatagg attctgatgg tcattggttt
     3001 caaattatcc cacaaaaata cggctctggc agcgggatgc cggttaaagg ttgttaaggc
     3061 tcagaggctg atactctcgc ccagcatggc aatatggacg tcgttatagc cttccgaaag
     3121 cagcttccct ctgaatgact ccagggcatc atcatcgccg tgtaccagaa acagtttgcg
     3181 aagtttgtcc ttctgctgag aacgcagata atggatcatc tcctggtagt caccatgcgc
     3241 actgaaacta tccatgatct caatacgtgc gttgacctgt aattcctgcc cgaacaggcg
     3301 aatggtcttc actctgctgc gaagtgcacc gccggtagtt ccggggcctg cataaccaac
     3361 aataagtatg gtgttttcag gatgttctat attgttgaac aggtgatgcc tgatccgtcc
     3421 ggcgttcatc ataccggaag cggaaatgat gatacaggct tttttattgt tgagcgattt
     3481 actttcatcc acagaacgaa catatctgag tttttcaaat ccaaatggat tcggatccct
     3541 gatcatatat cgcagcaact ccttgtcaaa gcactccgga tgctgctcaa agatggcagt
     3601 ggcattgacc gccaggggcg aatccacgta caccggaata tcaggcagcc tgccttccgt
     3661 ttgaagctgg tccagcatat agacgatctc ctgggttcta cctacactga atgctggtat
     3721 gatcagtttg cctttctgta ccacgcaggt ctgttgaatg atctccagca ggtgctctct
     3781 ttccttaggc gcttcatcat gcagccggtt gccataagtg ctttcacaga tcaggtaatc
     3841 acagggaggc atgggatcgg ggtcgcggag tatgggccgc tcgggtctgc caatatcagc
     3901 cgtaaacccc aggtaggtgg tcttgaggcc ttcgcggtga attcgcagtg ttacggaagc
     3961 actgccaaga atatgaccgc tgtctttgaa cagcacttcc acatcaggat gtacatggaa
     4021 ccagtgatca tagcccacca ttctgaacaa ctccatggtt ttggttacgt cttctacatt
     4081 atagaggggt tcttccttat gctcctcctc cattttccgg ttggtaaaat ctgattccct
     4141 ttcctgaata tgcgcactat ccagcagaag tattgcggtg aggtcgcggg ttgccggagt
     4201 ggcatagatc tttccctgaa atccctcttt gaccagcctg ggaagcttac ccgaatgatc
     4261 gatatgcgca tgtgataaaa tgaccagatc gatatcgcct ggtttaaaga agaaatgccg
     4321 attgaaattt tcatatgctt cttccctgcc ctgatataat ccgcaatcca gcagaatgcg
     4381 aaacccatca tccagggtca gcaaatgaga actacctgtc accgtaccgg ccgctccaca
     4441 aaatttgatg ttcatttgat gatcgttgtt cgttatgcgt ttttcgttat tcgttgttcg
     4501 ttattcgttc ctccccgctc ccgctcccac tcccactccc actcctcact cccactctca
     4561 ctctcactct cattctcact
//
LOCUS       DAOMAX010000216         4565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3327_length_4565_cov_4.090244, whole genome shotgun sequence.
ACCESSION   DAOMAX010000216 DAOMAX010000000
VERSION     DAOMAX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_4565_cov_4.090244"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PK742_10235"
     CDS             <1..404
                     /locus_tag="PK742_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77010.1"
                     /translation="AIGAFPAGWVADKLGIKRTFLTGLLLYILVYVGFAVVSGGTWYW
                     GLFFLYGLYAAATEGISKAWITLRVPKEETATAVGLYASLNSMAALFASSAAGLIWWQ
                     AGPGWAFGTTACMALAAFSYLLMTKENTNIA"
     gene            428..1726
                     /locus_tag="PK742_10240"
     CDS             428..1726
                     /locus_tag="PK742_10240"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQU77011.1"
                     /translation="MNKLLRRYRLTSSNLLIRGITLGGKFIFIIFLAKHLTTDQLGEW
                     GIFTTSIALSLYLIGLDFYTFSTRSLLEHPVAERGVMLRDQLLFYLLGYIILFPLLGL
                     LFVFKLMPLQYLVFFYVILVLEHFAQESYRIFVVFSKPVVANVILFLRTGSWAYALVL
                     LWMAGVDDVKYVKAAFFFWMGGGIAAMLTAVYFYGKLGLHIARKTPVNWKWIRQGLRI
                     SMMFFMATIAFKIIELADRYFIDHYQGKEEVGIYTFYANMANLIEIIGHTAVVIIFSP
                     KLIEYFHTSNRHYRLTHAAFARNMVGFTMLAAVFLAITIVPIISFLHKDEFYQELPVF
                     AVMCLSEMVFNFTLIFHYILYVRKRDRSIVWATIIAAVVNMALNFLLIPPYGIMGAAL
                     STLASMMLILVFKIILSRDVPETRQIIYLKFIRQRMRRKG"
     gene            1723..3090
                     /locus_tag="PK742_10245"
     CDS             1723..3090
                     /locus_tag="PK742_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HQU77012.1"
                     /translation="MNRRNLLQRIWQVLFLQESGIRKYFRNTSWLLGARIFQMLVALV
                     IGGLVARYLGPEKYGTYNYVISFVAIFSALSTMGIATVVVKDMVAEETDYQTAMGTGF
                     MLKLAGSSLAFLLVLACTFFVEDAEAVRMLLLIAGLQTIIKSTEIINSYFQARVESRK
                     AVRAQMISLILISALRLYGIAEEKELSWFLWMLPLDALVIGGIMLWYMRNIGLGVYTW
                     HSSFSYGKRLLSESWPSLFSGIFVTIYMKIDQVMLQELLNAEEVGYYAAAVRLSEVWI
                     TVPWIISGSLFPAMVNAAKESEQAFHTRINQAYILLISMALLVALPVTFLSKPVIHLI
                     FGQVYDPSVGVLQIHIFSCLFIFLGSVSNRWLVIRKQQRYWMINSGLGALANILLNLW
                     MIPKWGIQGAAWATLISYAFAYYFAYALFPRTRHIFSSQSANFFRVLTILPAWREIKK
                     LQQRL"
     gene            complement(3091..4065)
                     /locus_tag="PK742_10250"
     CDS             complement(3091..4065)
                     /locus_tag="PK742_10250"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU77013.1"
                     /translation="MEHRMGAPVKNHSSVQERMMDSPDISVVIPAYQSEQFIEACIRS
                     VLQHDCVKQVVVADDASTDDTAAIIQRLAEEDQRVEYTTHADGKNKGAGPTRNMAIGV
                     CRYAWIAFLDADDLWLPERFDQTFSVLHAYPDADGVYECLGIQFDTEEAEQQWMERHG
                     YTLTTLQERVEPESLIFSMAPLGNKGWFSGDALLVKRTMLEVCGGYSDLRLSQDTELF
                     MKLAVKGRLYPGNINTPVALRRVHGNNRITTSPERFYAHRLILWKQFSDWCLQEKLHG
                     KLLQKVWLMRTHDILVYVRWVPGNLARFIFTWRSLRPMLHPRWLKTFF"
     gene            complement(4008..>4565)
                     /locus_tag="PK742_10255"
     CDS             complement(4008..>4565)
                     /locus_tag="PK742_10255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU77014.1"
                     /translation="FYHPLPPSTTFTIAPDAALYLLRHIMEQQPRLIVELGGGLSTIL
                     MAKLIKSKGFDTRIITIESDAVYGEALRKELQQHGVEDLTDLRICPIEPTSLGTWYSI
                     AGNMPKDIDLLLIDGPPKAVAKDSRYPAFPFFEKYLSADASVIADDADRPDMLRTISR
                     WQQEYTQWNTEWVRLSRTIAVFRRG"
BASE COUNT         1050 a         1135 c         1107 g         1273 t
ORIGIN      
        1 atgccattgg agccttccct gctggttggg tggcagataa actggggatc aaacgcacct
       61 tcctgaccgg attgctgctc tacatactcg tgtatgtcgg gttcgccgtg gtttcaggag
      121 gcacgtggta ctggggattg ttctttttgt atggactata tgctgccgca acggaaggta
      181 tttctaaagc atggatcacc cttcgggtgc ctaaggaaga aacggccaca gcggtgggct
      241 tatatgccag cctcaacagc atggctgcat tgtttgccag ttcagctgct ggactcatct
      301 ggtggcaggc cggaccggga tgggcatttg gcactacggc atgtatggct ctggcagctt
      361 tcagctacct gttgatgacc aaagaaaata ctaacattgc atagcgtctc aggcaatccg
      421 ctgcccattg aataaactgc tccggcgata caggcttacc tcttcgaatc tgctgatacg
      481 cggtatcacg ctgggcggga aattcatatt catcattttc ctggccaagc acctcactac
      541 tgaccagctg ggtgagtggg gtatcttcac cacgtccatt gccctgagtc tgtaccttat
      601 cgggctggac ttctatacct tctccacccg cagcttgctg gaacatcctg tggctgaaag
      661 aggtgtcatg ctgcgtgatc agctgttgtt ctacctgctg gggtatatta tcctcttccc
      721 gttgttaggt ctgctgtttg ttttcaaact catgcccctg cagtaccttg tcttctttta
      781 tgtgatcctg gtgctggaac attttgcaca ggaatcttac cgcatctttg tggtcttctc
      841 caaacctgta gttgccaatg ttattctctt cctgagaacc ggctcctggg cttatgccct
      901 ggtactgctg tggatggctg gtgtggacga tgtaaaatat gtcaaagctg cattcttctt
      961 ctggatgggt ggtggcatcg ctgccatgct gactgctgtc tatttctatg gaaaactggg
     1021 gttgcatatt gcacgcaaaa cgcctgtcaa ctggaaatgg atacgccagg gccttcgcat
     1081 cagtatgatg ttcttcatgg ccaccattgc ctttaagatc atagaactgg cagacaggta
     1141 cttcattgat cactaccagg gaaaagaaga agtaggtatc tatacattct atgccaatat
     1201 ggccaacctg atagagatca tcgggcatac ggcagtggtc atcatctttt cgcccaaact
     1261 gatcgagtac ttccatacca gcaaccggca ttaccggctc acccatgcgg cctttgcccg
     1321 gaatatggtt ggcttcacaa tgctggctgc cgtctttctg gccatcacca tagtacccat
     1381 tatttcattt ctgcataagg atgaattcta tcaggagctg cctgtatttg ctgtcatgtg
     1441 tttatctgaa atggtattta acttcacttt gatattccat tatatattat atgtcagaaa
     1501 acgtgatcgc tccatagtct gggctaccat cattgctgcc gtggttaata tggcattgaa
     1561 ttttctgctc ataccacctt atgggatcat gggggctgcg ctttccacgc tggccagcat
     1621 gatgctcata ctggtattca agatcatcct gtctcgtgat gtgccggaaa ccagacagat
     1681 catctatctg aaattcatac gtcagagaat gaggaggaaa ggatgaaccg tcgcaacctg
     1741 ctgcaacgca tctggcaggt gcttttcctg caggaatcgg gaatccgcaa atatttccgc
     1801 aacacttcct ggttgctggg tgcccgtata tttcagatgc tggtggcact ggtcattggc
     1861 ggactggttg cacgctacct cggccccgaa aagtatggca cctacaacta tgtgatcagc
     1921 tttgtggcca ttttctctgc cctgtcaacc atgggaattg ctacggtggt ggtgaaagat
     1981 atggtggcag aagagaccga ttaccagacc gcaatgggca ccggtttcat gttgaaactt
     2041 gccggaagca gcctggcatt tctgcttgtt cttgcctgta ctttttttgt ggaagatgcc
     2101 gaagcggtca ggatgctgct gctgatagcc ggactgcaaa cgatcatcaa atctaccgag
     2161 atcatcaaca gctactttca ggccagggtg gaaagcagaa aggctgtgcg ggcacaaatg
     2221 atcagcctga tactgatcag cgctttgcga ctgtatggca ttgctgaaga aaaagaactt
     2281 tcctggtttc tctggatgct gccgctagac gcccttgtaa ttggtggcat catgctctgg
     2341 tatatgcgga acataggtct cggggtctat acctggcatt cttctttcag ctatggaaaa
     2401 agattgcttt cagagtcctg gccttctctg ttctccggca tcttcgtgac catctacatg
     2461 aagatagatc aggtcatgct gcaggaattg ctgaatgccg aagaggtagg atactacgca
     2521 gctgcagtac ggctgagtga ggtctggata accgtaccct ggatcatcag cggatctctc
     2581 tttcctgcaa tggtgaatgc agccaaagaa agcgaacagg catttcatac ccgcattaac
     2641 caggcttata ttctgctgat atccatggca ctccttgtag cattgcctgt tacctttctt
     2701 tcgaaaccag ttatccatct tattttcgga caggtctatg acccttctgt aggagtattg
     2761 caaatacaca tcttcagttg tctgttcatt tttctcggat cggtttccaa ccgctggctg
     2821 gtcattcgga aacaacagcg ctactggatg atcaactctg gtctgggtgc actggccaat
     2881 attctgctga acctctggat gatacccaag tggggcattc aaggtgcagc ctgggctacg
     2941 ctgatctcct atgcattcgc ctattacttt gcttacgcac tattccctcg aaccagacat
     3001 atcttttcca gccagagcgc caatttcttt cgggtgctta ccatccttcc ggcctggcgt
     3061 gagatcaaaa aactgcagca aaggctttga tcagaaaaag gtcttcagcc agcgagggtg
     3121 caacattggc cgaagagaac gccaggtgaa aataaatctt gccagatttc cgggtaccca
     3181 cctgacatat accagaatat catgtgttcg catgagccat accttttgca ggagctttcc
     3241 gtgcaatttt tcctgcaggc accaatcaga gaactgcttc cacaggatca atctgtgcgc
     3301 ataaaatctt tcaggagaag tggtgatgcg gttgtttccg tgtacccttc gcagagctac
     3361 cggagtgttg atatttccgg ggtacagcct gcctttgact gccagtttca tgaacaactc
     3421 tgtatcctga ctcagccgaa gatcagaata acctccgcat acttccagca ttgttctctt
     3481 aacaagcagc gcatctccgc tgaaccagcc tttgttaccc aaaggagcca tggaaaaaat
     3541 caggctttca ggttccactc tttcctggag tgtggtgagc gtataaccgt ggcgttccat
     3601 ccattgctgc tcagcttcct cagtatcaaa ctgtatgccc aggcattcat atacaccatc
     3661 ggcatcagga taagcatgca gtactgaaaa ggtctgatcg aatctttccg gtaaccacag
     3721 atcatcggca tccagaaatg cgatccaggc atatctgcaa acaccaattg ccatatttct
     3781 ggtaggacct gctcctttat ttttaccatc ggcatgcgtc gtgtattcta ccctttggtc
     3841 ttcttcagca aggcgctgta tgatagcggc ggtatcgtct gtggaagcat catcggccac
     3901 caccacctgt tttacacaat catgctgcag tacagagcgg atacaggctt caatgaattg
     3961 ttcagattga taagccggta tcactaccga tatgtcgggc gaatccatca tcctctcctg
     4021 aacactgcta tggttcttga caggcgcacc cattctgtgt tccattgggt gtactcctgc
     4081 tgccagcggg aaatggtgcg caacatatcc ggcctgtctg catcatcagc aatgacagag
     4141 gcatcagccg acagatattt ttcaaaaaag gggaatgccg ggtatcgtga gtcttttgcc
     4201 actgctttgg gcggaccatc gatcaataac agatcgatat ccttcggcat gttaccggct
     4261 atcgaatacc aggttccgag gctggtaggt tcgatcggac atatgcgcag gtctgtcagg
     4321 tcttccacgc catgctgctg caattccttc ctgagtgcct cgccgtacac tgcatctgac
     4381 tcaatggtaa tgatgcgggt gtcaaagcct ttgcttttta tcagtttggc catcaggata
     4441 gtggataatc cgccgcccag ttctacgatc aggcggggtt gttgttccat gatatgtcgc
     4501 aacaggtaca aagctgcatc aggcgcaatg gtgaaagttg tagatggagg cagggggtga
     4561 tagaa
//
LOCUS       DAOMAX010000217         4510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3403_length_4510_cov_4.418406, whole genome shotgun sequence.
ACCESSION   DAOMAX010000217 DAOMAX010000000
VERSION     DAOMAX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_4510_cov_4.418406"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..585)
                     /locus_tag="PK742_10260"
     CDS             complement(4..585)
                     /locus_tag="PK742_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HQU77015.1"
                     /translation="MKKSRLEAFSDGVLAIIITIMVLEMKIPHGTAWSDLEPILPVFG
                     SYVLSFIYLAIYWNNHHHMFQVVEKVNGAVLWANMALLLCLSLIPFTTAWMGENHFHG
                     NPVALYGMVLLLAGFSYFLLTLALKRIHHDESHFARATADQRKEMVSTILYIIAVVCA
                     FFVEWASIVIYILVAIMWVIPDKRMERILLDNK"
     gene            complement(1321..2235)
                     /locus_tag="PK742_10265"
     CDS             complement(1321..2235)
                     /locus_tag="PK742_10265"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77016.1"
                     /translation="MYTFDCTIVTDHRWLDESNADWYARQVWVEDAAVREALERRGWK
                     VHRTNWDDASFNWSTTRYAMIRSTWDYFHRFDEFFSWLEASSAKTTFINNISLIRENI
                     DKHYLLKLAGKGVPIPPTLFIEPGDARSLIEIARLSGWSEIVLKPAVSGAGRHTYRFL
                     PDDASKHENIFRELIAKESMLIQEFQHRIMHAGEVSLMVMGGQFTHAVMKKAKQGDYR
                     VQDDFGGTLHHFEPDNSLIAFAEQIVAACDPMPAFARVDIIRNNADEPVLSELELIEP
                     ELWFRHHPAAADVLAEYVVGMDQPDAKK"
     gene            2434..4230
                     /locus_tag="PK742_10270"
     CDS             2434..4230
                     /locus_tag="PK742_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77017.1"
                     /translation="MKRLLLLFIPLPVLAQVNLAPNPSFEEYSTCPTFYSTVDVYTDP
                     YVTDWFRSNDGSSDYMNACDVGETVGVPTNIFDDYCPARTGVAYAGFWSYLSSGIYRE
                     YMQAQLTEPMVADGCYYVEFYVTPATKSDFTTNNWTCDCIGMYISEVRPLSTLYTYTP
                     QITTAPGAFYTDTVGWTQIAGIYIADGGEEWISIGNFNNDAGTDVFDFLDEGASPYVY
                     MFADDVLVTELSDLDILSDTTLCAGDVWVLNAPGGADSYLWNTGETTTSITVTTTGDY
                     WVEMTTPCGTFLDSAYIFFSVDEEITSSTDIDVCYQDMPYIIDPGVAYETQVWSTGET
                     TPTLEVDAEGVYILNGYGSCQAYVDTFHLNVIEEVPDPMLLEDTLICLAEWTLELEVP
                     GFNTYDWSTGETTAAVTVSEEGTYTVTYSTGCDTYTDEMTITTDPYLLVDPDLGPDTL
                     VCEILGSLILDAGPDLPDYNWNTGETTQTITVTEEGAYWVLSTSLCGTHGDTIIVSPC
                     LFLNVPNAFSPNDDGLNDVFRVLCTDCENFLSLSIYNRWGDMIFETSDYTVGWDGTYQ
                     EKEQPIGSYVFMLRYLEGDQEAIIQGSVTLIR"
BASE COUNT         1124 a         1132 c         1098 g         1156 t
ORIGIN      
        1 accttatttg ttatccagca gaatgcgttc catgcgtttg tcgggaatga cccacataat
       61 ggctacaagg atgtagatca caatgctggc ccactctaca aaaaaggcac aaacaaccgc
      121 gatgatatat agaatagtgc tgaccatttc tttgcgctga tctgcagtgg ccctcgcaaa
      181 atgactttca tcatgatgaa tgcgtttcag tgcaagcgtg agcaggaaat aagagaaacc
      241 cgccagcagg agcaccatgc catacaaggc caccggatta ccgtgaaaat ggttctcgcc
      301 catccaggcg gtggtgaagg gaatgagcga aaggcacagc aagagcgcca tattggccca
      361 gagcaccgca ccgttcacct tctccactac ctggaacata tggtgatggt tgttccaata
      421 gatggcgagg tagatgaagc tcagtacata ggatccgaat accggcagga tgggttcgag
      481 atctgaccag gctgttccgt gggggatctt catttccagc accatgatgg tgataatgat
      541 ggccagaacg ccatcggaga atgcttcgag tcgcgatttc ttcatgtggt ttgggttggt
      601 ggagaaaaga taaggataaa caagaggaaa atgttcaaaa tggaaatgga acacagttta
      661 cactttgata cacagttcat acagatttac ggtcaaacat ccaccgtgaa tgcctctccg
      721 gcccccgagc cggagtcacg catctccatt agcatgcctc tccgggcctg acccggagtc
      781 tccctcacta tggttggaat aaagcctgcc gtcaggctgg ggctttctct acagatgaga
      841 tccaatacat gccaatcgaa gacactctta gcgtcctttg cgtattcttt gggtctttgc
      901 gtgtagattt taacacaaag gcgcaggtca tcgctgcgct ctgacaaaca caaagggctc
      961 aaagccagat actactctcc attagcatgc ctctccgggc ttgacccgga gtccccctca
     1021 ctatggttag aataaagcct gccgtcaggc tggggctttc tctacagatg agatccaata
     1081 catgccaatc gaagacactc ttagcgtcct ttgcgtattc tttgggtctt tgcgtgtaga
     1141 ttttaacaca aaggcgcagg tcatcgctgc gctctgacaa acacaaaggg ctcaaagcca
     1201 gatactccac tttttagcat gcctctccgg gcttgacccg gagtcccctt ctctatggtt
     1261 tgtaaaaagg cccgccggca ggcaggtcaa gcccggagaa acacaaattg aggagtgttt
     1321 ttattttttt gcatccggtt gatccatccc aaccacatac tccgccagta catcagctgc
     1381 tgcaggatgg tgccggaacc acagctccgg ttctatcagt tccagttctg aaagcaccgg
     1441 ctcatctgca ttgttccgga tgatatccac cctggcaaaa gcaggcatcg ggtcgcatgc
     1501 agccacgatc tgttcagcga atgcaataag agagttgtcc ggttcaaagt gatgcagggt
     1561 tccgccaaaa tcgtcctgta cacgatagtc tccttgcttt gcttttttca tcaccgcatg
     1621 ggtgaactgt ccgcccatta ccatcagcga aacctctccc gcatgcatga tgcgatgctg
     1681 aaactcctgg atcagcatgc tttctttggc tatcagttcc cggaaaatat tttcatgttt
     1741 gctggcatca tccggaagaa agcgataggt atgtcggcct gcgcctgaca ctgcaggctt
     1801 taatacgatc tcactccaac ctgacagacg tgctatctcg atcaaacttc ttgcatcacc
     1861 gggttctatg aaaagggtag gggggatggg aacaccttta cctgccagtt tcaagaggta
     1921 gtgtttgtct atgttctctc tgatcagcga gatgttattg atgaaagtgg ttttggccga
     1981 tgatgcttcc agccaactga agaattcatc aaagcgatga aagtaatccc aggtactgcg
     2041 gatcatcgca taacgggtcg tgctccagtt gaacgaagca tcatcccagt tcgtgcggtg
     2101 cactttccat ccccgtcgtt ccagtgcttc cctcaccgcc gcatcctcca cccacacctg
     2161 gcgtgcatac cagtcggcat tgctttcatc cagccagcga tggtcggtca ctatggtaca
     2221 gtcgaaggtg tacatgggcg caaagttatt ttctttggag atttgaatga cccggatgac
     2281 tgggagcact ggaaaatgaa gtgtcactcc cgcacctgta tgaccatttc cgggaaggct
     2341 ttccgcacca aagtgttctc ctctcttgca ttttcatgac atataaaatt caaccttctg
     2401 ctctttttct gtacatttac taacctgaaa accatgaaaa gactcctcct tctgttcatt
     2461 ccgctgccgg ttctggcgca ggtcaacctg gcacccaatc ccagctttga agaatattct
     2521 acctgtccga cattttattc gacagtagat gtgtacacag atccatatgt gaccgactgg
     2581 ttcaggtcta acgatggttc atcagattat atgaatgcct gcgatgtggg agagaccgtt
     2641 ggcgtgccta ccaacatctt tgatgattat tgtccagccc gcaccggcgt agcatatgca
     2701 ggcttctggt cgtatctgtc gtctggaatc taccgcgaat acatgcaggc acaactcacg
     2761 gagccgatgg ttgccgatgg ttgttactac gttgagttct atgtgacccc ggccacgaag
     2821 tctgacttta ccaccaacaa ctggacctgc gattgtattg gcatgtatat ctcagaggtg
     2881 cggccgctgt caaccctata tacctatact ccgcagatca ccactgcacc cggtgcattc
     2941 tatacggata cggtagggtg gacacagata gcaggtattt acattgctga tggtggggaa
     3001 gaatggatat ccattggcaa tttcaacaac gatgctggaa cagatgtctt tgattttctg
     3061 gatgaaggcg catctcctta tgtctatatg tttgccgatg atgtgctggt aactgaatta
     3121 tctgatctgg atatcctgtc cgatactaca ttgtgtgccg gcgatgtatg ggtgttgaac
     3181 gcaccaggcg gagcagattc ttacctgtgg aatacgggtg aaacgaccac ctccattacg
     3241 gtgaccacca cgggcgatta ctgggtggaa atgaccacac cctgcggcac gttcctggat
     3301 tctgcctata tctttttcag cgtggatgaa gagatcacca gttccacaga tatagatgtc
     3361 tgctatcagg acatgcccta tatcattgat ccgggagtag cttatgagac acaggtgtgg
     3421 agtactggtg aaacaacacc taccctggaa gtggatgcag aaggagtgta tattctgaat
     3481 ggatatggaa gttgtcaggc ctatgtagat accttccatc tgaatgtgat cgaagaggtg
     3541 cctgatccta tgctgctgga agataccctg atctgcctgg ctgaatggac cctggaactg
     3601 gaagtgcccg ggttcaatac ctacgactgg agcaccggtg aaaccacagc tgctgttacg
     3661 gtgagcgagg aaggaaccta tactgttacc tacagcaccg gttgtgatac ctataccgat
     3721 gaaatgacca tcaccacaga tccgtatctg ctggtggatc ctgacctcgg tccggatact
     3781 ctggtgtgtg agatactggg gtcgctcata ctggatgctg gtcctgacct gccagactat
     3841 aactggaaca ccggcgaaac tacccagact attacggtga cagaagaagg ggcttactgg
     3901 gtattgagta cctccctgtg cggcacccat ggagacacta tcatagtatc gccctgtctg
     3961 ttcctgaatg tacccaatgc attttcaccc aatgatgacg gactcaatga tgtattccgg
     4021 gtgttgtgta cagactgtga aaatttcctg tcgctcagta tttataaccg ctggggcgat
     4081 atgatctttg aaacaagcga ttacaccgtc ggatgggatg gcacctatca ggaaaaagaa
     4141 caacctattg gttcttatgt gttcatgctg cgttacctgg aaggtgatca ggaagctatc
     4201 attcagggct cggtgacact gatacgatag gttatcagca gggagattct gctgcatgga
     4261 aaggcctaaa ggcccggaga aacaccgtta ataaatagtt tataaaaatt ataatgcttc
     4321 tccgtccccc gagccgcctg cctgccatct gccaactcca ttaggatgcc tctccggccc
     4381 ccgagccgga gtctccttct ctatggtttg taaaaaggcc cgccggcagg caggcagtct
     4441 cattttttta aagtaccatc aaacaacagg cagcctgaag gttttatctc tacaagaaag
     4501 agggagactc
//
LOCUS       DAOMAX010000218         4508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3407_length_4508_cov_3.779025, whole genome shotgun sequence.
ACCESSION   DAOMAX010000218 DAOMAX010000000
VERSION     DAOMAX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_4508_cov_3.779025"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..708)
                     /locus_tag="PK742_10275"
     CDS             complement(40..708)
                     /locus_tag="PK742_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77018.1"
                     /translation="MKTFILAVSITALASGCSSGSGGEDAARSAVGGEHAGRYHIKSG
                     TLTYEANLPQDMGTNKRVVYFDDYGQKEKMVTITSISMMGQNIDKETHLITQDGFSYT
                     WSPGETSGMKVAVDDTFDPAAADFSKLTEEAMAKYGLTKEGDETIDGRKCDVWAMDNQ
                     GVQGKVWIWNNIPVKTSANMMGFSIIEKLVSLDEGDVDQAHFVLPDLTWKDMTYQSVP
                     GMGQ"
     gene            complement(749..3229)
                     /locus_tag="PK742_10280"
     CDS             complement(749..3229)
                     /locus_tag="PK742_10280"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU77019.1"
                     /translation="MFLKSLTLFLVLIPSLAFAQDKAFIYGTITNEKGKPVSDVTVVI
                     VGEAGVGTTTTSEGYYGLEVPAGDTLLLGFSAVGYGNQIKSVFPVSGGSYRMNVSLVT
                     GENELGPVVITESRRNELIMSIPIEEIEYIPDPSGDPIIAILKKYVTSNNELSAQYSV
                     RGGNYDENLVYVNDFEIYRPLLVRSGQQEGLPFPNYDLVGNITFSAGGFEARYGDKLS
                     SVLDIQYKRPDSLEAGVSASLLGGSFFVNNRYKDDTANGSRSYFLFGTRYKTNQYLLG
                     SLQTTGEYRPVFFDVQGLWGHDFSDHATLEVLANLSSSQYSFVPLSRSTTTGVVNNVI
                     RLDVFFEGQEITRFLTGFTGVSYQYRPNNSTGYKILASAYRSLEDETFDIIGDYWLGD
                     VETNLGDENFNEVVASRGSGTIQNFGRNYLDVLVLNLGHEGYHERGAHYIRWGGRLQG
                     EQIQDDLKEWEMLDSAGYSLPYTNELVSINSYYRSLLTLESLRANAYVQDTWNPGKES
                     DFYFTYGVRSSYWTINDEVTITPRVQAAWKPNWYREDSSKVDIVWKAALGMYNQPPFY
                     RELRNQEGLINYDVVSQKSIHALVSADYNFRAWNRDFKFIAEAYYKYLYDLVPYDVDN
                     LRIRYFGQNMANGFATGLDLRLHGEIVEDADSWVSLSLLRTMENLEGDSTYIDTDPGE
                     EVSLMLVEQGNIRRPTDQFLTFGMFFQDYMPGNKNFKVHLNFLFGTGFVTGPPGNPYL
                     RSSKTMPPYRRVDIGFSALLLDKKRERGDKIWSDVESLWAGIEVFNLLGIQNTISYLW
                     VEDLNNEQYAFPNYLTDRRINFRLIMKI"
     gene            complement(3261..3920)
                     /gene="rpe"
                     /locus_tag="PK742_10285"
     CDS             complement(3261..3920)
                     /gene="rpe"
                     /locus_tag="PK742_10285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQU77020.1"
                     /translation="MSHLVAPSILSCDFAHVGRDVEMVSSSGADWIHVDVMDGMFVPN
                     MSFGLPVVEAMKRHSSIPLDVHLMVAQPERYLSHFKEAGADVITIHLEACIHLHAAVQ
                     QIHKLGAKAGVAINPSTPIFQLVEILPDIEVVCLMSVNPGWGGQSFIPGSLSRISRLK
                     ALIGKTNSKALIEVDGGVKLDNAQEILNAGADVLVSGSGVFGQPDPAAAIQTLKNLKK
                     A"
     gene            complement(3957..>4508)
                     /locus_tag="PK742_10290"
     CDS             complement(3957..>4508)
                     /locus_tag="PK742_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77021.1"
                     /translation="DATIEVIGTNDYGLSDTITFNITLLAIPEIPEVYLYEFEIDDYG
                     CLEVIDPDLALTYTWWLVGEQIGTGVTVCADYGSPVILIAENAAGCTASTLQFIDWPH
                     DIKDILSEVSIEPNPVADVLSIKWTGPAQPARYFITDHVGRQVIAFGMMEEHMELDTS
                     QWPAGIYYLRADGWQGAIQIVKQ"
BASE COUNT         1111 a         1155 c         1080 g         1162 t
ORIGIN      
        1 aagcataaaa aaaacccggc ctttagccgg gttttttatt tactgcccca tgccgggaac
       61 cgactgatag gtcatgtctt tccaggtcag atcaggcaac acaaaatgtg cctgatccac
      121 atcgccttca tcaagtgaca ccagtttctc tatgatggag aaacccatca tgtttgcgga
      181 ggtcttcacc ggaatattgt tccatatcca gaccttaccc tggacaccct gattatccat
      241 tgcccataca tcacatttac gtccatcaat ggtttcatcg ccttctttgg tgagtccgta
      301 ctttgccata gcttcttcag taagcttgga gaaatctgcc gctgcaggat caaatgtatc
      361 atcaacagct actttcattc ctgacgtttc gcctggcgac caggtgtagg aaaaaccatc
      421 ctgagtgatc aggtgtgtct ccttatcgat attctggccc atcatggaga tgctggtaat
      481 ggttaccatt ttttctttct ggccatagtc atcaaagtac actacacgct tattcgtacc
      541 catgtcttgc ggcaaattcg cttcataggt cagtgtacct gatttgatat ggtagcgccc
      601 tgcatgttcg ccaccaacgg cagaacgggc tgcatcttcg ccaccggatc cggaagaaca
      661 cccggatgca agtgctgtga tggacacagc aagaatgaaa gttttcatgt tcataattta
      721 aaatgaaagt aataaagatc ctgaagaatc agatcttcat gataagccgg aagttgatac
      781 ggcggtctgt cagatagttc ggaaaggcat attgttcgtt attcaggtct tccacccaga
      841 ggtaggagat ggtattctga atacccagca gattgaacac ttctatacct gcccagagtg
      901 attccacatc tgaccatatt ttatctccac gttcacgttt cttatccagg agcagggctg
      961 aaaacccgat gtcaaccctt ctgtagggcg gcatggtctt actgcttctc agatacggat
     1021 tgccgggagg gccggtcacg aaaccggtac cgaacagaaa gttgaggtgt accttgaaat
     1081 tcttgtttcc cggcatgtaa tcctgaaaga acatgccaaa ggtcaggaac tggtctgtag
     1141 gcctgcgtat attcccctgt tccaccagca tgaggctcac ttcttcgccc gggtcggtat
     1201 caatataggt actgtcgccc tccaggtttt ccatggtgcg caacagacta agcgaaaccc
     1261 atgagtcagc atcttccacg atctctccgt gcagcctcag gtccagtccg gtggcaaaac
     1321 cgttggccat gttctgacca aagtaccgga tgcgaagatt atcgacatcg tacggcacca
     1381 ggtcatagag gtatttataa tatgcttctg ctatgaattt gaaatcgcgg ttccaggcac
     1441 ggaaattata atcggcggaa acgagtgcat gaatactttt ttgcgatacc acgtcatagt
     1501 tgatcagacc ttcctggttg cgcagttcgc ggtaaaacgg aggctggttg tacataccca
     1561 gtgctgcttt ccagactata tccacttttg aagaatcttc cctgtaccag ttgggtttcc
     1621 aggcagcctg cacccttggg gtgatggtca cttcatcatt gatggtccag taggatgaac
     1681 gcacaccgta ggtaaaatag aaatcagatt ctttacccgg gttccaggta tcctgaacat
     1741 aggcattggc ccgaaggctt tcgagcgtga gtaaggagcg gtaataggag ttgatgctca
     1801 ccagttcatt ggtataaggc aacgaatacc cggccgagtc cagcatctcc cattccttaa
     1861 gatcatcctg tatctgttcg ccctgcagcc ttccccccca tcgtatataa tgtgcacctc
     1921 tttcatggta tccttcatgc cccagattga gtaccagcac atccagataa ttccggccga
     1981 aattctggat ggtaccgctt ccgcggctgg ccaccacttc attgaaattc tcatcaccga
     2041 gattggtctc cacatctccc agccagtaat cgccgatgat atcaaaggtc tcatcctcca
     2101 gagaacggta ggcagaagcc aggattttat accctgtgct gttgttgggt ctgtactggt
     2161 aagaaacgcc ggtaaagcct gtgagaaagc gggtgatctc ctgcccttcg aagaatacat
     2221 ccagtctgat cacattgttg accactccgg tggtggtgct tcgtgacagc ggcacaaaac
     2281 tatactgact gctggagaga ttggccagca cttccagggt ggcatgatcg ctgaagtcat
     2341 ggccccacaa accttgcaca tcaaaaaata ccggtctgta ttctccggta gtttgcagtg
     2401 atccgagaag atactgattg gtcttatatc gtgtaccgaa cagaaaataa gatctggaac
     2461 cgttggcagt atcatctttg taacggttgt tcacaaaaaa acttcccccc aacagagagg
     2521 cactcacgcc ggcttccagt gaatccggtc tcttatactg aatatcaagt acactcgaaa
     2581 gtttatcgcc gtagcgggct tcaaagccac cagcagaaaa ggtgatgttg cctaccagat
     2641 catagttggg gaaaggcaag ccttcctgtt gcccgcttct taccagcagg ggccggtaga
     2701 tctcaaagtc gttcacatag accaggttct catcgtagtt tccgccacgc accgaatact
     2761 gcgcactgag ttcattgttg gaggtgacgt atttttttag aatggcaatg atgggatcgc
     2821 cggaagggtc aggaatgtat tcgatctctt cgatggggat ggacatgatg agttcgtttc
     2881 gtcttgattc tgtgatcacc acagggccaa gttcattttc gcctgtaaca agactcacgt
     2941 tcatgcgata gcttccaccg gaaacaggga aaactgattt gatctgattg ccgtatccta
     3001 ctgcgctgaa gcccagcagc agggtgtcgc cggcaggcac ttcgagacca taataacctt
     3061 cggaggtggt agtggtaccc acgcctgcct cgcccacgat gaccaccgtg acatcactga
     3121 ccggcttgcc tttctcattg gtgatggtgc cgtaaatgaa tgctttatcc tgggcaaatg
     3181 ccagcgaagg aattaataca aggaacagcg ttaggctttt caggaacatg cgcatcatta
     3241 acggccgcag gcgggcttca ttatgccttc ttcaggtttt tcagggtctg gatggcagca
     3301 gcaggatccg gctgaccaaa tactccgctt cctgacacca gcacatcagc tccggcgttc
     3361 aggatctcct gggcgttgtc cagcttcacc cctccgtcca cttctatcaa agccttgcta
     3421 ttggttttac caataagcgc ctttagcctg cttatcctgg acagggaacc cggtatgaag
     3481 gactgcccgc cccagcccgg attcacagac atgaggcaaa ccacttcaat atcaggcagg
     3541 atctctacca actggaagat cggtgtggat gggttgatgg ctaccccggc tttcgctccc
     3601 agcttgtgga tctgttgcac agcggcatgc aggtgtatgc aggcttccag gtgaatggtg
     3661 atcacatctg ccccggcttc cttgaaatgc gacaggtagc gctccggctg ggccaccatg
     3721 aggtgcacgt caagcggaat ggagctgtgg cgtttcatgg cttccactac cggcagtcca
     3781 aagctcatat tgggtacgaa catgccgtcc atcacatcca catggatcca gtctgcaccg
     3841 gatgaagaga ccatttccac gtcacggccc acatgggcaa aatcgcagga taagatagaa
     3901 ggcgctacga gatgactcat gctccaaaga taggggttct gcagctcagc acaggcctat
     3961 tgtttgacga tctgaatggc tccctgccag ccatcggcac ggaggtagta aatacctgca
     4021 ggccactggc tggtgtcgag ttccatgtgt tcttccatca ttccgaatgc gatgacttgc
     4081 cgacctacat gatctgtaat gaagtatctg gcgggttgtg caggtcctgt ccatttaatg
     4141 gataagacat ctgcaacagg gtttggctca atggacacct cgctgagaat gtctttgata
     4201 tcatgtggcc agtcgatgaa ttgaagtgtt gatgctgtac atcctgcagc attctcagcg
     4261 atcaaaatga cagggcttcc ataatcggca catactgtta ctccggtgcc gatctgttca
     4321 ccaaccaacc accaagtata ggtcagagca aggtcagggt ctattacttc caggcatccg
     4381 taatcatcta tttcgaactc gtacaaataa acttcaggta tctccggaat agcaagcagg
     4441 gtaatgttga atgtgatggt atcagataat ccatagtcat tggtaccgat cacctcgatg
     4501 gtggcatc
//
LOCUS       DAOMAX010000219         4506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3410_length_4506_cov_4.321950, whole genome shotgun sequence.
ACCESSION   DAOMAX010000219 DAOMAX010000000
VERSION     DAOMAX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3410_length_4506_cov_4.321950"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PK742_10295"
     CDS             <1..625
                     /locus_tag="PK742_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQU77022.1"
                     /translation="LMHLAERHGFKVNTFTHILEGYKVADKMAEHGAGGSTFSDWWAY
                     KYEVIEAIPYNAALMDQMGVVTAINSDDAEMARRLNQEAAKAIKYGGLTEEEAWKLVT
                     LNPAKLLHIDDRVGSVKTGKDADLVLWNDNPLSVYARAEKTFIDGRLYYDREEEARKQ
                     SAMQAERRRLVDRAANAGKKGERTSPVIIIQEPEYHCDTEGVFTTEQ"
     gene            666..1919
                     /locus_tag="PK742_10300"
     CDS             666..1919
                     /locus_tag="PK742_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQU77023.1"
                     /translation="MSVTGAQNDTPVDSPTVVMNATIHCGDGRVIENGLMAFYEGKIT
                     FLADARLVKFDFRGYRVIDAAGKHVYPGLIALNSQLGLREIEMVRSTNDQRETGYLNP
                     NVRSVIAYNTDSRVIPTVRSNGILLAQIVPQGGRMPGTSSVMKLDGWNWEDAVYALDN
                     GLHLNWPGYMQGRWNNGFRYEPDPDYENKIQELVQLFSEARVYEQQHKDEPVNLKLEA
                     IGPVFQGEKQLFIRANKLREISNAVAFAESFGIRPIIVGGEESWMMADELASKEIPVV
                     LEAVDRLPVSPDEHTSGPYELPAVLTKSGVTCAISVSNDGSSFWNMRNLPFQAGTAAG
                     YGLSKEEALTCITLNPARILGIDDHTGSLDLQKDATFLISEGDLLDMRSSKVIQAFIQ
                     GQEVELHNWQEALFNKFRKKYGLTD"
     gene            complement(1971..2441)
                     /locus_tag="PK742_10305"
     CDS             complement(1971..2441)
                     /locus_tag="PK742_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQU77024.1"
                     /translation="MPSFVKKPRQAPGINTASLPDIVFILLFFFMVATRMRETDIKVK
                     QVLPQATEMEKLEKKDLLSYIYIGAPEKRYQKELGTAPRLQLNDQLADVEDIAVFVEA
                     EKEKMPDENRAKIIMALKVDKEVNVGIISDVKTELRKANALKLTYITNKRMEEN"
     gene            complement(2449..3000)
                     /locus_tag="PK742_10310"
     CDS             complement(2449..3000)
                     /locus_tag="PK742_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQU77025.1"
                     /translation="MGKKTRGTPEINASSMADIAFLLLIFFLVTTEIAIDEGINVVLP
                     PWTNEPPPPIETNNRNTLIVNLNARDQLQVEEELTDVRMLRDLTKQFINNNGVDPHQS
                     DNPQVAVVSFKGDRGTSYDMYIQVYNELRGAYNDLRDEAAKRKFGKEFTELTDTTKIN
                     EIKDMYPIRISEAEPSEFGAGTK"
     gene            complement(3007..3327)
                     /locus_tag="PK742_10315"
     CDS             complement(3007..3327)
                     /locus_tag="PK742_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77026.1"
                     /translation="MENFLVNIVLPGAVFLAIIAVILFVASLLIGIFQNIRGSIKLLA
                     ALGLLIILFIIGYATASDANPTSIDLASGTIKMISAGIITMLALTVITVVTTVVMSIY
                     NLFK"
     gene            complement(3346..4080)
                     /locus_tag="PK742_10320"
     CDS             complement(3346..4080)
                     /locus_tag="PK742_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQU77027.1"
                     /translation="MKKLFALIATMGLAMFATTQVVMAQAEEAAEASGEGGITLIKRY
                     FIEGDYIFMSLPLICLILGLAFCIERIITLSLADVNTKNLMKSVETAVNNKDVEKAKQ
                     ICKATRGPIASIMYQGLSRTDEGIDIVEKSVSSYGSVQMGLLEKGLSWISLFIALAPM
                     LGFFGTVIGMVQAFDDIASAGAISATIVAAGIKVALLTTVFGLIVAIILQIFYNFIVA
                     KIEGIVNKMEDNSITFIDILVKNKIA"
     gene            4284..>4506
                     /locus_tag="PK742_10325"
     CDS             4284..>4506
                     /locus_tag="PK742_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77028.1"
                     /translation="MTSFLQDRVCCSGQFSIGGLRFAAGGRILGDQVSTYKLATCQLF
                     YTLLLFFTFNSQTGLLRDPLSGGGLNLLTR"
BASE COUNT         1173 a         1045 c         1145 g         1143 t
ORIGIN      
        1 gctgatgcat ctggcagagc gccacggatt caaggtgaac accttcaccc acattctgga
       61 aggctacaag gtagctgata aaatggcaga acacggggca ggcggttcta cattctctga
      121 ctggtgggcc tataaatatg aggtcattga agccattccc tataatgccg cactcatgga
      181 tcagatggga gttgtaaccg ccatcaacag tgatgatgcc gagatggcac gacgcctcaa
      241 ccaggaagcc gcaaaagcta ttaagtacgg tgggcttacc gaagaagaag cctggaagct
      301 ggtaaccctg aatccagcca aactgcttca tatagatgac cgtgtgggaa gtgtgaagac
      361 aggaaaggat gcagatctgg tattgtggaa tgacaaccct cttagtgtat atgcacgggc
      421 agaaaagact tttatagatg gccgattgta ttacgacaga gaggaagaag cccgcaagca
      481 atctgctatg caggcagagc gcagacgtct ggtcgatcgg gcagcaaatg caggaaagaa
      541 gggagaaagg accagtccgg tcatcatcat acaagaaccg gaatatcact gtgatacaga
      601 aggtgtattc accacagaac aatgagcata tgaaatacta catattttta atattacttt
      661 ttttagtgtc tgttacagga gctcagaatg atacgcctgt agattcacca actgttgtca
      721 tgaatgccac catccattgt ggtgatggac gggtgataga gaacggactc atggcttttt
      781 atgaaggaaa gatcactttt ctggcagatg cccggctggt caaattcgat tttcgcggat
      841 accgtgtcat agatgccgct ggtaaacatg tatatccggg tctgatcgct ctcaacagcc
      901 agttgggatt gcgtgagata gaaatggtaa gatctaccaa tgaccagaga gaaacaggtt
      961 atctgaatcc gaatgtgcgg tcggtcatcg catacaacac agacagccgg gtcatcccta
     1021 cagttcgttc caatggcata ttgctggcgc agatagtacc acagggcggg cgcatgccgg
     1081 gcacttcttc agtcatgaag ctggatggct ggaactggga agatgcggta tatgccttag
     1141 acaatggtct gcatctcaac tggcccggtt acatgcaggg aaggtggaat aatggatttc
     1201 gctatgaacc ggatcccgac tatgagaata agatacagga gctggtgcag ttgttcagtg
     1261 aagccagggt gtatgaacaa cagcataaag acgaaccggt caacctgaaa ctcgaggcga
     1321 tcgggccggt attccagggc gaaaaacaac ttttcatacg tgccaacaag cttcgtgaga
     1381 tcagtaatgc agtggcattt gcagaatctt tcggcatcag gcccatcata gtaggcggag
     1441 aagaatcctg gatgatggca gatgagctgg cctctaagga gataccagtt gtactggaag
     1501 cggtagatcg gctaccggta agcccggatg aacacacttc aggtccatac gaactgccgg
     1561 ctgtgctgac caaaagcggt gtgacctgtg ccatatcggt cagcaatgat ggttcatctt
     1621 tctggaatat gcgcaacctg ccctttcagg caggcacagc ggccggttat ggcctgagta
     1681 aggaagaagc acttacatgt atcactttga atcctgcaag gatactgggt atagatgacc
     1741 acaccggatc actggacctt caaaaagacg ccacctttct catcagcgaa ggcgacctgc
     1801 tggacatgcg aagcagtaag gtcatacagg cattcatcca ggggcaggaa gtagaactgc
     1861 ataactggca ggaagcgctt ttcaataaat tccggaagaa gtacggattg acagactaaa
     1921 aaaaagtccc ggcttgcacc gggaccatca ccaactaaaa tgttttcagg tcaattttct
     1981 tccatccgct tgttcgtgat gtaagtcagc ttcagcgcat tggctttacg cagttctgtt
     2041 ttcacatcac tgatgatacc cacgttcacc tctttatcta ctttgagggc catgatgatc
     2101 ttggctctgt tctcgtcggg catcttttcc ttttcagctt ctacaaatac cgcgatgtct
     2161 tccacatctg ccagctggtc attcagctgc aggcgggggg ccgttcccag ctctttctgg
     2221 tagcgctttt cgggtgcacc aatatagatg tagctgagca ggtctttctt ctccagtttt
     2281 tccatttcgg tggcctgggg aagcacctgc ttcaccttga tatcggtttc ccgcatacgg
     2341 gtggccacca tgaagaagaa caggaggatg aacacgatat ccggcagtga agccgtattg
     2401 ataccgggtg cctgtctggg tttctttaca aaactgggca tatcctgatt atttggtacc
     2461 ggcaccgaac tcagaaggct ctgcctctga gatgcggatg gggtacatgt ctttgatttc
     2521 gttgatcttg gtcgtgtcgg tcagttctgt gaactccttg ccgaatttcc gtttggcggc
     2581 ttcgtcccga aggtcgttgt aggcacctct cagttcattg tacacctgta tatacatgtc
     2641 ataagaagta ccacggtcac ctttaaatga tacaacagct acctgcggat tgtcagactg
     2701 atgaggatcc acaccgttgt tgttgatgaa ctgtttggtc agatcacgca gcattcgcac
     2761 atctgtcagt tcttcttcca cctgcaactg gtcgcgggca ttgagattca cgatcagcgt
     2821 attgcgattg ttggtttcaa tgggtggagg gggctcattc gtccatggcg gcaggaccac
     2881 attgatccct tcgtcaatgg cgatctcagt ggttaccaga aagaatatca gcagcaggaa
     2941 ggcgatatcg gccatcgagc tggcgttgat ttcaggtgta ccacgtgttt tcttacccat
     3001 actgttttat ttgaacaggt tatagatcga catgaccaca gtggtcacca ccgtgatcac
     3061 tgtcagagcc agcatggtga tgatacctgc agagatcatt ttgattgttc cgctggcaag
     3121 atcgatactg gttggattgg catcggatgc agtagcatag ccgatgatga ataagataat
     3181 gagcagcccc agggcagcca gcagtttgat ggacccgcgg atgttctgga agatccctat
     3241 gagcagggat gccacgaaca ggatgaccgc tatgatggcc agaaaaacgg cgccgggaag
     3301 gactatatta acaaggaaat tttccatgtt ccgtttttta tcggattagg caattttgtt
     3361 cttaaccagg atatcgatga aggtgataga gttatcttcc atcttgttca cgataccttc
     3421 gatcttggca acgataaagt tgtagaagat ctggaggatg atggccacga tcagaccaaa
     3481 tacggtggtc aacagggcca ctttgatacc ggcggccacg atggttgcag agatggcacc
     3541 ggcactggca atatcatcga atgcctgcac cataccgatc accgttccga agaatccgag
     3601 catcggagcc agcgcaatga acagagagat ccaggaaagc cctttttcca gcagacccat
     3661 ttgcacagag ccataagatg acactgattt ttctacaata tctattcctt catctgtacg
     3721 agacagaccc tggtacatga tagaagctat cgggccgcgg gtagctttac atatctgctt
     3781 ggctttttcc acgtccttgt tattcactgc agtttctacg ctcttcatca gattcttggt
     3841 attcacatca gccaggctga gggtgatgat acgttcgata caaaaagcca gacccaggat
     3901 cagacagatc agcggaagcg acatgaagat gtagtcacct tcaatgaaat aacgcttgat
     3961 cagggtgata ccaccttcgc cggaggcttc ggcagcttct tctgcctgag ccatcactac
     4021 ctgggtggta gcgaacatag ccagtcccat tgtggcaatc aatgcaaaca actttttcat
     4081 acaggttctt ttgttggtga ttaaagattg aattgggcta caaataaagc aattttaatt
     4141 gttgccgtga aataggctac acaaatttaa agtaggcctg aggctatgga aaatgcgctg
     4201 tctatttcac cgacggccta aaattaggcc attttactca atgaacggct taccacttac
     4261 ggatgtgatg cagacaaggc aatatgacat ctttcttaca agaccgggtc tgttgcagcg
     4321 ggcagttttc aattggcggc cttcgctttg cagcgggtgg tcgaatttta ggtgatcagg
     4381 tcagcacata taagcttgcc acctgtcagc tcttttacac ccttctactc tttttcacct
     4441 tcaactccca gaccggattg cttcgcgatc ccttaagtgg gggggggctt aacctgctca
     4501 cccgcc
//
LOCUS       DAOMAX010000220         4481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3439_length_4481_cov_4.476502, whole genome shotgun sequence.
ACCESSION   DAOMAX010000220 DAOMAX010000000
VERSION     DAOMAX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_4481_cov_4.476502"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1684)
                     /locus_tag="PK742_10330"
     CDS             complement(203..1684)
                     /locus_tag="PK742_10330"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQU77029.1"
                     /translation="MIRKILVANRGEIAIRVMRTAKEMGIATVAVYSEADRNAMHVRF
                     ADEAVLLGPPPSNQSYLLGDKIIAVAKETGADAIHPGYGFLSENAAFSRKVQEAGLIF
                     IGPDPESIERMGSKIEAKQAVKDFGIPMVPGTDHAISDIKEALDVAEKAGYPVLIKAS
                     AGGGGKGMRVVQNAAEMEEQMHRAMSEAESAFGDSSVFIEKFVTSPRHIEVQVMADRH
                     GNVIYLFERECSIQRRHQKLVEEAPSAVVSESMRKAMGESAVNVARACNYVGAGTVEF
                     LVDADLNYYFLEMNTRLQVEHPVTEFITGLDLVRMQIEVAQGDELKVKQEDLRIHGHA
                     IELRVTAEDPANNFLPDVGKLITYRRPQGAGVRVDDGYEEGMDIPIYYDPLLSKLIVH
                     AENRQLACERMVRAIDEYTISGAATTLPFGRFVMQHEAFLSGQFDTGFIERYFKPDML
                     QREEEDELAVAAYVATHLQSRPTAKQTTQQAAQVSNWKKNRIG"
     gene            1736..2395
                     /locus_tag="PK742_10335"
     CDS             1736..2395
                     /locus_tag="PK742_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77030.1"
                     /translation="MGKRSAIGVLFMRELRSEWRNKYAMSGILLYLLSIIFLITFTFE
                     ETDDRTWMVLYWIAILFTSVNAVAKSFLQESEGRQYYFYTLASPQHIILAKMAYNAVV
                     MVVMSGLALLFYIATQGYPVEDDASFLLAMLLGALAFASTFSMISAVASRAANNGTLT
                     AILSFPILLPVMRILIRVSLLALTGQNLAMNAQDLLYLAALNIFIVVLALILFPYLWR
                     D"
     gene            2402..3094
                     /gene="ccsA"
                     /locus_tag="PK742_10340"
     CDS             2402..3094
                     /gene="ccsA"
                     /locus_tag="PK742_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQU77031.1"
                     /translation="MINQLHKRWWKVLGAVLMLYTFTAGMMIQVPARFVLHETIRNLF
                     FHVPVWFTMILLLLVSLVQSVRFLRTGKMVHDIMAAETAAVAVLFGCLGFLTGMLWGS
                     FTWGDTFSWLFADTKILGAFIGLMIYAAYFILRGSINDEEKRARVAAVYSIFAYMLLI
                     VFIFVIPRLTDSLHPGNGGNPAFSTYDLDNSMRTVFYPAVFAYFLTGLWIASLRVRVR
                     ILHYRKHHINVL"
     gene            3091..3309
                     /locus_tag="PK742_10345"
     CDS             3091..3309
                     /locus_tag="PK742_10345"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HQU77032.1"
                     /translation="MRFFIRLLAVLLTALHPAWLLAQSSDQAEMATGLRSNGLIWIVV
                     GVILIILCGLLLYLYNLDRRITNMEKEQ"
     gene            3331..>4481
                     /locus_tag="PK742_10350"
     CDS             3331..>4481
                     /locus_tag="PK742_10350"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQU77033.1"
                     /translation="MSNISFLESVVQNFEKAADITGHPRGLLDQIRACNSVYRMKFPV
                     KIKGEFRVIEAWRVQHSHHRTPTKGGIRYDNMVNEDEVMALASLMTYKCALVDVPFGG
                     AKGGIKINPREYTEEQLERITRRYAAELVKKNMLGPGLDVPAPDYGTGAREMSWIVDT
                     YMALHPDSVDGYGCVTGKPVTQHGINGRTEATGLGVFYGVKEACTYKDDMEALGLTPG
                     VEGKKVIVQGFGNVGYYSAKFFQEGGAIIVGIGEYEGAIYNENGLDYEAVFNHRKSTG
                     SILNFPGATNMEKTSDILEQPCDILIPAALENVITKDNAPRIKAKIIGEAANGPITSD
                     ADDILNGMGIMILPDMYLNAGGVTVSYFEWLKNLSHVRFGRMSKRFEQA"
BASE COUNT         1018 a         1177 c         1104 g         1182 t
ORIGIN      
        1 aagaaatgtg gaaatgaggt aatgtgaaaa tgagttaatt gaacactccc gtcatgtgcc
       61 cggggtatga cttcaaagac agaaaagtta cattctgcat ttccacattc tcccgttttc
      121 tcattttctc attttcccat tttcacattc tcccattttc acattctctc attttcacat
      181 tttcacattc tcttattcac ctctacccta tcctgttttt tttccagttg gagacctgcg
      241 ctgcctgctg cgtagtttgt ttggctgttg gtctggattg gaggtgggtg gctacgtaag
      301 ctgccactgc cagttcatct tcttcttccc gttgaagcat gtctggtttg aagtaacgct
      361 caatgaaacc ggtgtcaaac tgtccgctca gaaaagcttc atgctgcatg acgaatctgc
      421 caaagggcaa ggtggtggca gcaccgctga tggtatattc atcgatggct ctcaccatgc
      481 gttcacaggc cagttgtctg ttctctgcat gcacgatcag tttgctcaat aacgggtcat
      541 aataaatggg aatatccatc ccttcttcgt acccgtcgtc cacccggacg cctgctccct
      601 gcggtcttcg gtaggtgatc agtttgccca catcgggcag gaagttgttg gcggggtctt
      661 cggcagtaac acggagttcg atggcatgac catgtatgcg caggtcttcc tgttttactt
      721 tgagttcatc gccctgtgcc acttctatct gcatgcgcac aaggtccagt ccggtgatga
      781 actcggtgac cggatgttcc acctgcaggc gggtattcat ttcgaggaag tagtaattca
      841 ggtcggcatc taccaggaat tcaacagttc cggcacccac atagttacag gctctggcca
      901 cattgaccgc actttcgccc atggccttcc gcatgctctc gctgaccact gcactgggtg
      961 cttcttccac cagtttctga tgccttcgct ggatgctgca ttctctttca aagaggtaga
     1021 tcacattgcc atgtctgtcg gccatcacct gcacttcaat atgcctcgga ctggtcacga
     1081 atttctcgat gaagacagaa ctatcgccga acgcactttc cgcctcactc atggcacgat
     1141 gcatctgctc ttccatttct gcagcattct gcaccacacg catacctttc ccacctccac
     1201 cggcactggc tttgataagg acaggatagc cggctttttc agccacatcc agggcttctt
     1261 taatatcgct gatagcatga tctgttcccg gcaccatggg aataccaaaa tccttcaccg
     1321 cctgcttggc ttcgatctta ctgcccattc gttcaatgct ttcaggatcc ggaccgataa
     1381 agatgagccc ggcctcctgc accttcctgg agaaagcggc attctcactg agaaatccgt
     1441 aacccggatg gatggcatca gcaccggtct ctttggcaac ggcaatgatc ttatcgccca
     1501 ggaggtaaga ctgattactg ggcggcggac ccagcagcac ggcttcatct gcaaagcgca
     1561 cgtgcatggc gtttctgtcg gcctctgaat ataccgccac ggtagcgatt cccatttcct
     1621 ttgcggtgcg catcacccgg atggcgatct ctcctctgtt ggcaaccagt atctttcgta
     1681 tcatgtgcca caaatctaaa gcatcacatt gatacgtttg gggtaatttc gccctgtggg
     1741 aaaacgttcg gccattggcg tattgttcat gcgtgagctt cggagtgagt ggcggaacaa
     1801 gtatgccatg agcggaatcc tgctctatct gctcagcatc atatttctga tcaccttcac
     1861 ttttgaagag accgacgacc gcacctggat ggtgttgtac tggatcgcca tcttgttcac
     1921 cagtgtcaat gcagtggcca agagttttct gcaggaatca gaaggcaggc aatattattt
     1981 ctataccctg gcctctccgc agcatatcat cctggccaag atggcttaca atgcagtggt
     2041 catggtggtg atgtcaggtc tggcactact tttttacatc gccacacagg gttatccggt
     2101 agaagacgac gcatcctttc tgctggccat gctgctgggt gccctggctt ttgcatccac
     2161 cttcagtatg atcagcgcag tggcatccag ggctgcaaac aacggcaccc tgacagccat
     2221 tctgagcttc ccgatcctgc ttcctgtcat gcgcattctg atacgtgtaa gcctgctggc
     2281 gctaaccgga cagaacctgg ccatgaatgc acaggatctg ctttatttag ctgcactgaa
     2341 catcttcatt gtggtgttgg cattgatatt atttccgtac ctttggcggg actaatcggg
     2401 gatgatcaac cagctgcata aacggtggtg gaaggtgctg ggagccgtgc tgatgctcta
     2461 cactttcaca gcaggaatga tgatccaggt acctgccaga ttcgtccttc acgagaccat
     2521 cagaaacctc ttttttcacg tacctgtctg gttcaccatg atcctgcttt tgctggtatc
     2581 gctggtgcag tccgtgcgtt ttttgcgcac cggaaagatg gtacatgata tcatggctgc
     2641 agagaccgct gctgtggccg tgttgtttgg ctgtcttggc tttcttaccg gaatgttatg
     2701 gggaagcttt acctgggggg atactttttc ctggttgttt gcagacacca agatactggg
     2761 tgccttcatc ggactgatga tctacgctgc ttatttcatt ttaagaggca gcatcaatga
     2821 tgaagaaaaa cgagccagag ttgctgctgt gtacagcatc ttcgcctata tgctgctcat
     2881 cgttttcatc ttcgtcatcc cgcgacttac agattccctg caccccggaa acggaggcaa
     2941 tcctgccttc agcacatatg atctcgacaa tagcatgcga accgtattct accctgctgt
     3001 gttcgcttat tttctaactg gcttgtggat cgcttccctg cgtgtcaggg tgcgcatcct
     3061 gcattacaga aaacatcata taaacgtatt atgagatttt ttatccgact gctggcggta
     3121 ctgcttaccg ctcttcaccc tgcatggctg ctggcacaaa gcagcgatca ggcagagatg
     3181 gccacaggac tgcgaagcaa cggactgatc tggatcgtgg tgggtgtgat actcatcatt
     3241 ctgtgcggtt tattgttgta tctatacaac ctggaccgcc gcatcaccaa tatggaaaaa
     3301 gaacaataaa ccctaaatac caacattcgt atgagcaaca tcagcttctt agaaagcgtc
     3361 gttcagaact tcgaaaaggc ggccgatatt accggccatc cgaggggtct cctcgaccag
     3421 atccgggcct gtaattctgt gtaccggatg aagtttcccg tgaagatcaa aggtgagttc
     3481 cgtgtgatcg aggcatggcg tgtgcagcac agccaccacc gcacacccac caaaggtggt
     3541 atccgctatg acaacatggt gaatgaagat gaggtcatgg cacttgccag tctgatgacc
     3601 tacaagtgtg cgctcgtaga tgtgccgttt ggtggtgcca aaggaggtat caagatcaat
     3661 ccgagagaat acaccgaaga gcagctggaa cgcatcaccc gccgttacgc tgcagaactt
     3721 gtgaagaaga acatgctagg tcccggtctc gatgtacctg ctcccgacta cggaacaggt
     3781 gcacgtgaga tgagctggat cgtagatacc tatatggcgc tgcatcctga ctctgtagat
     3841 ggctatggct gtgtgaccgg taagcctgtt acacaacatg gtatcaacgg acgtaccgag
     3901 gcaaccggtc ttggtgtatt ctacggtgtg aaggaagcct gtacctacaa ggatgatatg
     3961 gaagcactgg gacttacacc cggtgtggaa ggcaagaagg tcatcgtgca gggatttgga
     4021 aacgtaggtt actacagtgc caaattcttc caggaaggtg gtgccatcat cgtgggtatc
     4081 ggcgagtatg aaggtgctat ctacaatgag aacggactgg actacgaagc cgtgttcaac
     4141 caccgcaaga gtaccggttc catcctgaat ttcccgggtg ccaccaatat ggaaaagacc
     4201 agcgatatcc tggaacaacc ttgtgatatc ctgatccccg cagccctgga aaatgtgatc
     4261 accaaagaca atgcgccacg catcaaggcg aagatcatcg gcgaagccgc caacggaccc
     4321 atcacatcag acgctgatga catcctgaac ggcatgggca tcatgatcct gcccgacatg
     4381 tatctgaacg caggtggtgt gacggtatcc tactttgagt ggctgaagaa cctctctcac
     4441 gtacgcttcg gacgtatgag caagcgcttt gagcaggcca g
//
LOCUS       DAOMAX010000221         4457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3472_length_4457_cov_2.564289, whole genome shotgun sequence.
ACCESSION   DAOMAX010000221 DAOMAX010000000
VERSION     DAOMAX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3472_length_4457_cov_2.564289"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PK742_10355"
     CDS             complement(<1..250)
                     /locus_tag="PK742_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77034.1"
                     /translation="MTKTKQELLDQFATAALTALISKMPLYDSNGKYGEPIDDQELQT
                     IKKDMTATAYEYAGWMMIAREDSKKWLKEFLLCLCHDEI"
     gene            complement(247..678)
                     /locus_tag="PK742_10360"
     CDS             complement(247..678)
                     /locus_tag="PK742_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77035.1"
                     /translation="MEKQRKDRIREKAKNTVKFAKPFTKPPLVGKLRQYQDTIYGKLK
                     ARWWKEPENQVCVCREELGCCKPGDPLVVHHTAGRENWLLLYTPLWKPICTKAHRKIS
                     DDSSWGLARGYNVLRANRPRITITDIQPICDKYGVELKISS"
     gene            complement(756..980)
                     /locus_tag="PK742_10365"
     CDS             complement(756..980)
                     /locus_tag="PK742_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77036.1"
                     /translation="MKVIIEHTEETGWNVIHGDKVADRLSYDEMLGLVVAITIPDKRP
                     CLQWLKTKEQHEAYEKYLEEIREKNTEALK"
     gene            complement(1141..2532)
                     /locus_tag="PK742_10370"
     CDS             complement(1141..2532)
                     /locus_tag="PK742_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77037.1"
                     /translation="MKTNKRIGIGAGILAAIAGAVVFTPKATEHQQPVPETVWGVNGT
                     NADQFFGDIEAREYFQELNGAFRFRADKYVGLVPEDTLSDFVWFVGDQPVIYTVNVRG
                     NTAMENLYGLEYLIREGVNVIGVEMGNEEYAFYDFQFDDYAIGFVPTYAAIREAYPQL
                     PVGVFLAPRPKNSEIQGGRTDQQGYNDEAIDWFNAQMDPDLCVSWHIYNNHKDNPVLD
                     QPFPELAWDGGNIPDEVNNWYKQAFDSIAAYDNWKPVSDYLNQILPGREVWITEFGPT
                     NATGDRQNTWGYHACDFLTRYRAQDKGFAVVMKHNGISPTNAAIVSSPTRSDASQETL
                     VPRMGWYTAKLFSEAPGAYNVSRQADLPGTYQYWYINTGDTPIDIAGIVSNGMTVTSA
                     TTHYLQGPEWGSSSGAAPWMAKGSAPAQVHPGIITSAGSVFPAMSYGYCEITFSKPCQ
                     KPWWCFWRKCRCK"
     gene            complement(2542..2976)
                     /locus_tag="PK742_10375"
     CDS             complement(2542..2976)
                     /locus_tag="PK742_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77038.1"
                     /translation="MEAINEIIFFASLAFLLGAVIADQWHRKARKENTARIDAICERV
                     KLMTDHNYALAWEKLMMELVGEDRQENVRSVIKIMQGKLQARDQEIQDIRRYINAHPD
                     ESTADEVQALLHLLLAFPNVYDLTDQQKERMKQFIVDNPYLT"
     gene            complement(2976..3914)
                     /locus_tag="PK742_10380"
     CDS             complement(2976..3914)
                     /locus_tag="PK742_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77039.1"
                     /translation="MGTVQVQIIFYILIAINIVALIISGRNYFYQWVVEEPEEDDSIL
                     GKSLRKAEAEIKQAIKTLQTGKKEVAEAVVLTSERAAQSLLRITIDAGGEPLYGEIWQ
                     VKRETGRIDQFIVQRSDDTGVYVTPLKGNELPIDHGDIIERAGTTDTSSLQYWVEHVA
                     GNLQKLRSLHEGNGRHILIDCDGVLTDGRTTIGSDGQQFRHMSSRDNRAIRELVAKGW
                     TVVLVTASSWPYFKEYAEKTGCIFEQIRTKEELIEKYTPYVAVGDDTWDIELLKNAEQ
                     AFCPQDSDVSIIRLKNVTVLPAKGGDHVITHLLKRL"
     gene            complement(4023..>4457)
                     /locus_tag="PK742_10385"
     CDS             complement(4023..>4457)
                     /locus_tag="PK742_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77040.1"
                     /translation="VSEADVAAGSPKEGDMIAYNADNPDDRWLVAKAFFEANYEPAEQ
                     TEKALGNTDANGAKKNVKDIVFWGNGDLFKLISKASSQSEGWMKSTKAMETPFGVVVQ
                     VTTQQRNPDGSYAVAEALTFIPGAKVQEEKDGDGTVVARAIA"
BASE COUNT          957 a         1193 c          965 g         1342 t
ORIGIN      
        1 aaatttcatc atgacaaaga caaagcaaga attcttttag ccacttcttg ctgtcctctc
       61 tcgcaatcat catccatcca gcatattcat aggcggttgc tgtcatgtcc tttttgattg
      121 tttgaagttc ttggtcatca atgggttccc catacttccc attgctgtca taaagtggca
      181 ttttggaaat caatgccgtt aatgctgctg ttgcaaattg gtctaataat tcttgctttg
      241 tctttgtcat gatgaaattt ttaactccac cccatactta tcgcagatgg gctggatatc
      301 ggttattgta attctcggcc ggttggctct caggacattg tagcctctcg ccaatcccca
      361 tgaactatcg tctgatatct tcctgtgagc cttggtgcag ataggcttcc acagaggggt
      421 gtacagcaat agccagttct cccttccagc ggtgtggtgc accaccaatg gatctccagg
      481 cttgcagcat cccaattcct ccctgcaaac gcatacctgg ttctccggtt ctttccacca
      541 ccttgctttc agctttccgt agatggtgtc ctgatattgc ctgagttttc ccacgagagg
      601 aggttttgta aacggttttg cgaacttgac ggtattctta gccttttccc ttattctgtc
      661 cttgcgctgc ttctccattc tgcactcaaa actgcatacg ctctggccct gatatttcgg
      721 ggtgaacttc tttcggcatg atgggttagc acagatcatt ttaatgcctc agtatttttc
      781 tcacgaattt cttccaggta tttttcgtaa gcctcgtgtt gctcctttgt ttttaaccac
      841 tgcaggcacg gtcttttatc aggtattgtt atggctacta ccagaccaag catttcatca
      901 taagagagtc tgtccgcaac cttatcgcca tgaatgacgt tccatccggt ttcttctgta
      961 tgttcgatta ttactttcat ataaactgct ttttacaaca actacactca tagacactta
     1021 tccataccct acctgtgaaa tcttcaccta tgtacttaat aacacctaca ccgcagcttt
     1081 cgcaccttgg atgcagatta gtaaggaagt tggaaatgcg tcttaatatt tttgggttat
     1141 tcatttgcaa cgacattttc tccagaaaca ccaccagggc ttctggcagg gtttactgaa
     1201 tgtgatctca cagtatccgt agctcatggc agggaatacc gatcccgcac tggtgatgat
     1261 gcccggatgt acctgagccg gtgcactacc ctttgccatc cacggtgctg ctcctgaact
     1321 gctaccccat tccggcccct gcagatagtg agtggttgct gatgtcacgg tcatgccatt
     1381 gctgactatt cccgcaatgt cgataggtgt atctccggtg ttgatgtacc agtactgata
     1441 tgtaccgggc aaatcagcct gtctggacac attgtaagcc ccaggagcct cgctgaacag
     1501 tttggcggtg taccaaccca ttcttggcac caaagtctcc tgagaggcat cagatcgcgt
     1561 aggagacgat acaatagctg cattggtagg agatataccg ttgtgtttca tcaccaccgc
     1621 gaacccctta tcctgtgcac gatatctggt aaggaagtca caggcatggt agccccatgt
     1681 attctgccta tccccggtgg cattcgttgg cccgaactct gtgatccaga cctctctgcc
     1741 gggaagtatt tgattcaggt agtcgctgac cggcttccag ttgtcatagg cggcaatgct
     1801 gtcaaatgcc tgcttgtacc aattattgac ctcatcggga atattccctc catcccacgc
     1861 aagctccggg aatggctgat cgagtacagg gttgtccttg tggttgttgt atatgtgcca
     1921 ggatacgcat aaatccgggt ccatctgtgc gttgaaccag tctatcgcct cgtcattata
     1981 tccctgctga tctgtccggc ctccctgtat ctcgctgttc tttggccttg gagctaagaa
     2041 tacccctacc ggcagctgcg ggtaggcttc cctgatcgct gcataggtcg gtacaaatcc
     2101 gatggcgtag tcgtcaaact gaaagtcgta gaaggcgtat tcttcgttac ccatttccac
     2161 tccgatcaca ttaacgcctt ctctgatcag gtactccagt ccgtataggt tctccatagc
     2221 ggtattgccc cggacattga cggtatagat caccggctgg tcgccaacaa accaaacgaa
     2281 atcagatagg gtgtcctccg gcacaagacc aacgtacttg tcagcccgga agcgaaaggc
     2341 tccattgagc tcctggaagt actcccttgc ctcgatgtcg ccgaagaact ggtcggcatt
     2401 ggttccgttc accccccaaa ccgtttccgg tacaggctgc tggtgttctg tggctttggg
     2461 cgtgaacact accgccccgg ctattgctgc gagtatcccc gcaccgatgc cgattctctt
     2521 atttgttttc atctgagttc actatgttag atacggatta tcaactataa attgcttcat
     2581 cctctctttc tgctgatcgg tcaggtcata cacattcggg aacgccagta aaagatgcag
     2641 taacgcctgc acttcgtctg ctgtgctctc gtctggatgg gcattgatgt atctgcggat
     2701 atcctgaatc tcctgatcac gggcttgtag cttaccctgc atgattttta tgacagacct
     2761 gacattttct tgtctatcct ctcctaccag ttccatcatc agcttctccc atgctaatgc
     2821 gtagttatgg tcggtcatta atttgactct ctcgcagatc gcatcaattc tggccgtatt
     2881 ctcttttctc gctttgcgat gccactgatc agctattaca gcgccgagta aaaatgccaa
     2941 tgatgcaaaa aagattattt cgttgattgc ttccatcaca gtctctttag aaggtgagta
     3001 ataacatggt ctccgccctt cgcaggcaga accgtgacgt ttttaaggcg gatgatggac
     3061 acatcagagt cctgtgggca gaatgcctgc tctgcgttct tcagcagctc gatatcccat
     3121 gtgtcgtctc caacggccac atacggggtg tacttctcga tcagctcttc ctttgtccgt
     3181 atctgttcga agatgcagcc ggtcttctca gcatattcct tgaagtatgg ccatgaactg
     3241 gccgtcacca gtaccacagt ccatccctta gccaccagtt cccggatagc cctgttgtcc
     3301 ctgctgctca tgtgacggaa ttgctggcca tcgctgccga tagtcgttct accatcggtc
     3361 aacaccccat cacagtctat gaggatgtgc ctgccgttgc cctcatgtag gcttctcagc
     3421 ttttgaagat tgcctgcgac atgctctacc caatattgta aagagcttgt atcggttgta
     3481 ccggctcttt ctataatgtc accatggtca ataggtaatt cattgccttt caaaggagtt
     3541 acatatacgc cggtatcatc tgacctttgt actatgaatt gatctattcg acctgtttct
     3601 cgttttactt gccatatttc tccatataaa ggctctcccc cggcatctat ggtgattcgc
     3661 agtaaagact gagcggctct ttcagatgtc aaaacaaccg cttctgctac ttctttcttg
     3721 ccggtttgaa gtgttttaat ggcttgcttt atctctgcct ctgcctttcg aaggctttta
     3781 ccgagaatac tgtcatcttc ttctggttct tcgaccaccc attggtaaaa gtagtttctg
     3841 ccgctaataa tcagcgctac aatattgata gcaatcagga tgtagaatat gatttgtact
     3901 tgtactgttc ccataactca tgttttgcca gcgattgctc cggggatccg cacacccccg
     3961 aaactcgctg gaatcaaaaa agaccggttt ttaatgccag tcgtgcggaa ccggcttgtc
     4021 ttttaggcaa tcgccctcgc tactacagtg ccatcgccat ccttttcttc ctgcacttta
     4081 gcgccaggaa taaaggtcag ggcttccgca acggcatagc ttccatcagg gttacgctgc
     4141 tgagtagtca cctggacgac aacaccaaag ggcgtctcca tggccttggt ggacttcatc
     4201 caaccttcgg actgactgga agccttgctg atcagtttga acaggtcgcc gttaccccag
     4261 aacacaatgt ccttgacgtt ctttttggct ccgttggcat cggtattgcc cagagccttt
     4321 tcggtctgtt cagccggttc gtaattggct tcaaagaaag cctttgctac cagccacctg
     4381 tcatcagggt tgtcggcatt ataggcgatc atatcccctt ctttgggaga tcccgctgct
     4441 acatctgctt ctgaaac
//
LOCUS       DAOMAX010000222         4389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3555_length_4389_cov_3.868482, whole genome shotgun sequence.
ACCESSION   DAOMAX010000222 DAOMAX010000000
VERSION     DAOMAX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_4389_cov_3.868482"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK742_10390"
     CDS             <1..253
                     /locus_tag="PK742_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77041.1"
                     /translation="IMMSGQDNIKVAIDTIDNGAYDYIIKGESAYLRAQMIIDHIIRN
                     KNLSRYLRDSRRKENILIGIVIFMFFLLLLVSLASRGIV"
     gene            330..917
                     /locus_tag="PK742_10395"
     CDS             330..917
                     /locus_tag="PK742_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQU77042.1"
                     /translation="MHKTPNESLHRKSVSEFKDAEKTPIVVVLDNVRSLHNVGSVFRT
                     CDAFLVRGICLCGVTAKPPHRDIEKTALGATESVHWEYFDETTEAIRYLRSLNYRIIA
                     VEQTDSSISLEKYQPYANEKVALVFGHELYGVSEEVLALCDDCIEIPQFGTKHSLNIS
                     VSAGIVLWDLFSKLRFGVQIYTPAQPLKVVHGDHP"
     gene            971..1819
                     /locus_tag="PK742_10400"
     CDS             971..1819
                     /locus_tag="PK742_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQU77043.1"
                     /translation="MKYQGREQSKHVKDQRGKRPGTGMAVGGGAIIVLIIALLMGQDP
                     TQLLQQMGDTGQTSDEPYISSEKEDQLMEFVGVVLKDNEDVWNELFPEQLGRNYVEPD
                     LVVFTGGVQSACGMASSATGPFYCPGDDNVYLDLGFFDELSSRFGAPGDFAMAYVIAH
                     EVGHHVQDLLGITAQVDQYRGKVSEKEYNALSVKLELQADFLAGVWAHHAQAMKNILD
                     PGDIDEALRAANAIGDDRLQKQAQGYVVPDAFTHGTSEQRMRWFKKGYTTGLVSEGDT
                     FNARVL"
     gene            1859..2821
                     /locus_tag="PK742_10405"
     CDS             1859..2821
                     /locus_tag="PK742_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU77044.1"
                     /translation="MRILVFCGIRVIVMLIMLSGLPAIAQDQHLTVYFPFGASELDAD
                     ERKHVLYFLADTLDHDRIQSIEIRGYCDYIDNSSFNDSLSLARANHVAGLLKNNGIPS
                     QKISSVTGFGERKASEQKSSEAERQLQRRVEVDFICTCHGAAETTEAEKEEEKKQEIT
                     GDEVRVIQGDTVQDRKGVRYKGRYLVGDDFELNTFDIGDSIVLLRLHFQPGKHKLLRE
                     SVPTLYRLTDQMKANPNMHIIIVGHICCSPTSAPDGLDNDTGKYELSRNRAKEIYAYL
                     VSQGIEEERLAYAWMGPKDKLYPEERDAREMQGNRRVEIVVTAK"
     gene            2853..3737
                     /locus_tag="PK742_10410"
     CDS             2853..3737
                     /locus_tag="PK742_10410"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HQU77045.1"
                     /translation="MKKILVFILTISSLTVFSQQVRIPVFRDGKFGFADAAGIMVIPA
                     TYTIASPFSDGLAAVAITNDQQMWRYGFIDESGQWVIPPTYSAANAFGSGLAPVAMDN
                     FKWGFIDRNGETVIPGTYQLAYGFEQGTAQVQRKNQWGLINTAGEEVVPIQYYGVTNL
                     EKDVYAAQVTMYDPWFILDRQGNKVSEKGFGKVRDFDEGLAPARNEDELWGFVNTSGE
                     WVIEPAYTDAFSFSEGLAAVEVEYANWGFIDRTGKMIIEPQYDRGTLFRNGFAVAERG
                     LEFLVINKANQVQFTFSR"
     gene            3734..4285
                     /locus_tag="PK742_10415"
     CDS             3734..4285
                     /locus_tag="PK742_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77046.1"
                     /translation="MNTLIRITYVLLLLSFAGAAQAQLDSVMTAGSELQIRHRKKDKI
                     RTAEIGDYLVVKDQFAKYKGKLSALEEPYLIVLDDGQELDIRQIRSIRLDHSLFRVFS
                     GYTVMAVGGYTTFLGALSGVVGVLGILTSDFGALAVFGVILLPAGLLILAAGRAILHK
                     YNNGKRWSYQIAPLPGISTESDN"
BASE COUNT         1165 a          933 c         1160 g         1131 t
ORIGIN      
        1 gatcatgatg tccggacagg ataacatcaa agtagccatt gataccatag ataacggtgc
       61 ctacgattat attatcaaag gagaaagtgc ctatctgagg gctcagatga tcatagacca
      121 catcatccga aacaagaatc tgagccgcta cctgcgtgac agcagacgga aagaaaatat
      181 tctgatcggt atcgtcattt tcatgttctt tctgctgctg ctggtatcac tggccagcag
      241 gggtattgtc tgacatttct gttaaataac aggaacagaa aactactttc cggtcttctc
      301 cgttacattt gtatcccttt ttgccaggca tgcataaaac gcccaacgaa tcattacaca
      361 gaaaatccgt ttctgagttc aaagacgcag agaagactcc catcgtggtg gtgcttgaca
      421 atgttcgcag cctgcacaat gtaggttccg ttttccgcac ctgtgatgcg tttctggtgc
      481 ggggcatttg tctttgtgga gtgactgcca aaccacctca tcgtgacata gagaaaacgg
      541 ctcttggtgc taccgaaagc gtgcactggg agtactttga tgagaccaca gaagccatca
      601 ggtatctgag atcgctcaac tatcgcatca ttgccgttga acagacagac agcagcattt
      661 ctttagaaaa gtaccagcct tatgccaatg aaaaagtagc attggtattt gggcatgaat
      721 tgtacggtgt aagcgaggaa gtacttgccc tttgcgatga ttgcatcgag atccctcagt
      781 tcggtaccaa acattctctg aatatttctg tcagtgccgg tatcgtattg tgggatctgt
      841 tctccaagct ccgctttggg gtgcagattt atacacctgc ccagcctttg aaagtggtac
      901 atggcgatca tccctgaaga ggtgcatgct cctgtgaaaa atcgtatctt catgctataa
      961 taaatcctat atgaagtatc agggcaggga gcaaagcaaa catgtaaagg accagagggg
     1021 caaacgaccc ggaaccggaa tggctgttgg cggaggtgcc atcattgtgc tcatcattgc
     1081 attgctgatg gggcaggatc ctacacaact gctgcagcag atgggtgata ccggtcaaac
     1141 cagtgacgaa ccttatatct cctcagagaa agaggatcag ctcatggagt ttgttggtgt
     1201 agtgctgaag gataacgaag atgtatggaa tgaactgttc cctgagcaac ttggccggaa
     1261 ctatgtggaa ccggatctgg tggtctttac aggtggggtg caatcggcat gtggtatggc
     1321 cagcagcgcc accggaccat tctattgccc gggagatgat aacgtgtatc tggacctggg
     1381 tttctttgat gagctgtctt cacgctttgg cgctcccggc gattttgcca tggcctatgt
     1441 cattgcacat gaagtggggc atcatgtaca ggaccttctg ggcatcactg cacaggtaga
     1501 tcagtatcga ggaaaggtca gcgaaaagga atacaatgcc ctgtctgtaa agttggaatt
     1561 gcaggcagac ttcctggccg gcgtctgggc ccatcatgca caggccatga agaacattct
     1621 ggatccgggt gatattgatg aagccctgag ggcggccaat gccattggtg acgaccggct
     1681 tcagaaacag gctcagggct atgtggtgcc ggatgccttt acccatggaa catctgaaca
     1741 aaggatgcgc tggttcaaaa agggctatac taccgggctg gtttctgaag gagacacctt
     1801 taacgccaga gtgctgtaat ttttcgctgc gtaaagacct cctctgatca aaacactcat
     1861 gcgcattctt gtattctgtg gcattcgtgt tattgtgatg ctgataatgc tgtcaggttt
     1921 gccagctatt gctcaggatc agcacctcac cgtatatttc ccttttggag catctgaact
     1981 ggatgcagac gaacgaaaac atgtgctcta tttcctggca gatacgctgg atcatgacag
     2041 aatacaatct attgagatca ggggatattg cgattatatt gacaacagtt ctttcaatga
     2101 cagcctctcg ctggcccgtg ccaatcatgt ggccggtctg ctgaagaaca acgggattcc
     2161 ttcacagaag atcagttctg taacaggttt cggtgaaagg aaagccagcg aacaaaagtc
     2221 ttccgaagca gagcggcagc tgcaaagaag ggtagaagtt gattttatct gtacctgtca
     2281 cggagcagca gaaacaactg aggcggaaaa ggaagaggaa aaaaagcagg agatcacggg
     2341 cgatgaagtg cgggtcattc agggcgatac agtgcaggac aggaaaggcg ttcgatataa
     2401 aggccgctat ctggtgggcg atgattttga gttgaataca tttgatatag gtgatagtat
     2461 cgtgctcctt agattgcact ttcaacctgg taaacacaaa ttgctgcggg aaagtgtgcc
     2521 taccttatac aggctgacag accagatgaa ggccaatccg aatatgcaca tcatcattgt
     2581 tggacatatt tgttgttcac ccaccagcgc acctgacggg ctggacaatg atacgggtaa
     2641 atatgaactg agcagaaaca gagccaaaga gatctatgca tatcttgtaa gccagggcat
     2701 tgaagaagag cggcttgcct atgcctggat gggcccgaaa gacaaacttt atcctgaaga
     2761 acgtgatgcc cgtgagatgc agggaaacag acgggtggag atcgtggtta ccgccaagtg
     2821 atcatacttt ttatctttga acaaagagat taatgaaaaa gatacttgta tttatactga
     2881 caatcagctc actgactgtt ttttcacaac aggtgcgcat tcctgttttt agagatggaa
     2941 agttcggttt tgctgatgct gccggtataa tggtcatacc ggcaacctat accattgcat
     3001 caccgttctc agatggcctg gctgcagtag ccattaccaa tgaccagcag atgtggcggt
     3061 atggatttat tgatgagtcc ggtcagtggg tcataccacc tacctacagt gcagccaatg
     3121 cattcggaag cggactggct ccggttgcaa tggataattt caaatggggt ttcattgacc
     3181 ggaacggaga gactgtgata ccgggtacat accagcttgc atatggtttt gaacagggta
     3241 ctgctcaggt acaaaggaag aatcaatggg gtctgataaa taccgcaggc gaagaagtgg
     3301 taccgataca gtattatggc gtcaccaatc tggagaagga tgtctatgct gcacaggtaa
     3361 ccatgtatga tccatggttt attttagaca ggcaggggaa caaggtcagc gagaaaggct
     3421 ttggcaaggt acgtgatttt gatgaagggc ttgctcccgc caggaatgaa gacgaattat
     3481 ggggctttgt gaataccagt ggagaatggg tcattgaacc ggcttatact gatgcgttct
     3541 cattttctga gggtctggcc gcggttgaag tggaatatgc caattggggg tttatagatc
     3601 gtaccggcaa gatgatcatt gaaccgcagt atgacagagg aaccctcttt agaaatggat
     3661 ttgcagtagc agaaagagga ctcgaatttc tcgtgatcaa taaagccaat caggttcaat
     3721 ttacattcag cagatgaata cattaataag gattacatat gtcttgcttc tgctgtcatt
     3781 tgcaggtgct gctcaggctc agctggattc tgtaatgact gcaggcagtg agttgcagat
     3841 cagacatcga aaaaaagaca agatcaggac ggctgagatt ggagactacc tggtggttaa
     3901 agaccagttt gcaaagtaca agggaaaact gtcagccctg gaggaaccct acttgattgt
     3961 actggatgac gggcaggagc tggatatcag gcagatcagg agtatcagac tggatcacag
     4021 cctgtttcgg gtgttctccg gttatacggt aatggccgtt gggggctaca ctacttttct
     4081 gggggccctc agtggcgtgg tcggtgttct gggaatcctt acttcagact ttggggcgct
     4141 tgcggtgttc ggagtgatac tgttaccagc tggcctactg atcctggccg caggcagggc
     4201 catactgcac aaatacaaca acggtaaacg atggagctat cagatcgctc cgcttcccgg
     4261 aatatcaaca gaatctgaca actgatctgt ttatcggctg tctctttgct tgtttctgga
     4321 tgcgccctga cgaaacttat ttctgtttcg gttcctgttt ctgcgcttgc ctttgttttc
     4381 ttcagaagg
//
LOCUS       DAOMAX010000223         4356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3617_length_4356_cov_4.274587, whole genome shotgun sequence.
ACCESSION   DAOMAX010000223 DAOMAX010000000
VERSION     DAOMAX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3617_length_4356_cov_4.274587"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..624)
                     /gene="folE"
                     /locus_tag="PK742_10420"
     CDS             complement(13..624)
                     /gene="folE"
                     /locus_tag="PK742_10420"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQU77047.1"
                     /translation="MSNYKGYTKADSYDAETTEKLKAHYSQVITTLGEDITREGLEKT
                     PERIAKAMQFITQGYGQDAEAVVRSAMFEEPCSEMVIVKDIELYSMCEHHMLPFIGRA
                     HVAYIPNKYIVGLSKIARVVDIFARRLQVQERLTHQILDTLQNTLDPQGVAVVIEANH
                     LCMMMRGVQKQNSVTTTSAFTGEFQKSETRSEFINLIGASLHG"
     gene            complement(624..1034)
                     /locus_tag="PK742_10425"
     CDS             complement(624..1034)
                     /locus_tag="PK742_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQU77048.1"
                     /translation="MVYLTRKEHFNAAHRLYNQAWTEEKNAEVFGRCANKNWHGHNYD
                     LYVTVKGEPDPDTGFIMNAKELSALIKREVVERFDHRNLNVDVPYFRDIQPSTENFVK
                     VIWDILEPYIAGCRLHSIRLQETENIYCEYFGER"
     gene            1165..1812
                     /locus_tag="PK742_10430"
     CDS             1165..1812
                     /locus_tag="PK742_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQU77049.1"
                     /translation="MILYDQVFENNQRWVADKLRLQPEYFSELSSGQNPDILYIGCSD
                     SRVTAEEMTGVEPGQMFVHRNIANLVPNNDLSSSSVIEYAVAHLKVKHIVVCGHYYCG
                     GVKAAMQSADLGILNPWLRNIRDVYRLHKSELNAIADEGERYNRLIELNVEEQCINVI
                     KMATWQKSFLTTGGPQVHGWIFDVHSGQLKDLQLDLEEKLSDIRSIYDLGTTNNI"
     gene            1814..2848
                     /locus_tag="PK742_10435"
     CDS             1814..2848
                     /locus_tag="PK742_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HQU77050.1"
                     /translation="MLVYKGLNLRTLLVWTGFHYGWLFIGAAIIAAFYHFGHIRFLIP
                     WLPVSVIGTAVAFYVGFKNNQAYDRMWEARKIWGAIVNSSRAWGMYVDGFVTDQFSEK
                     RISDMELKAVKKRLIYRHIGWLYALRSQLLLTAPWEHSNQRGPVGRRARYYQKRFGVG
                     LVDDEVTRSELMNYLPADEYERLIKHSNTATQIINEQSRDLTELRKQELIEDFRHMEL
                     QQILYHFYEHQGKCERIKKFPIPRQYANMSRYFVGIFIILLPFSMIPELMQLGDWGLW
                     LSVPITALIGWVYLVMELLGDYSEFPFQGMANDIPMLSICRTIEIDLREMLNDEDIPP
                     PVQAVGGILM"
     gene            3148..>4356
                     /locus_tag="PK742_10440"
     CDS             3148..>4356
                     /locus_tag="PK742_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77051.1"
                     /translation="MELDDLLIQGADYAYTIHGWLKGVNSGALNTSRDMGGDAKSTGI
                     RKLMGTDAFGFILHYYDGDYTQIGSGNSFIPTASTSGTGYNLVTENLYNGNIRAMTTA
                     LMRETHAKVDVVGAAYTYDQLNRLTGRKTFIKSNMHLSGNFTWSAISESPKWSSAYTY
                     DGNGNLLTLERAGDNATSSYDMDDLTYNYYPNTNQLGRVDDAISSSAYSDDIDDQTGA
                     NYSYDEIGNLISDDQGDIDDIQWNVQGKITFIDKGSGPDLTFAYDAMGNRVMKMVDDG
                     TEQVYTYYVRDAQGNIMTTYSRIDNGSTDSIFLGEQHIYGSGRLGYLSTRISMDSVLP
                     TTGYYYRQLGLRRYELSNHLGNVLVVITDRRLLIEGTGGLAGKIVSAEPDVLQANDYY
                     PFGMMMSGRTW"
BASE COUNT         1117 a         1006 c         1075 g         1158 t
ORIGIN      
        1 cacgcatcgc tctcatccgt gcagcgatgc accgatcaga ttgatgaact cgcttctggt
       61 ctctgatttc tggaattcgc cggtaaaggc agaggtggtg gtcacgctgt tctgtttctg
      121 cacaccacgc atcatcatac agagatgatt ggcctcaatg accactgcaa ctccctgtgg
      181 gtcgagggta ttctggagtg tatccaggat ctgatgggtg agcctctcct gcacctgcag
      241 gcgcctcgca aagatgtcaa ccacccgtgc gatcttgctg agcccaacaa tgtatttatt
      301 cggaatgtat gccacatgtg cacgtcctat gaatggcagc atatggtgtt cacacatgga
      361 gtacaattca atgtctttga caatgaccat ctcactgcag ggctcttcga acatggcgct
      421 gcgtaccact gcttctgcgt cctgtccgta tccctgggta atgaactgca tggccttggc
      481 aatgcgttca ggtgtttttt ccaggccttc ccgggtaatg tcttcaccca gggttgtgat
      541 gacctggctg taatgtgctt tcagcttttc tgtcgtttcg gcatcatagc tgtctgcctt
      601 ggtatagcct ttgtagttac tcatcatctt tcaccaaaat attcacaata gatgttctcc
      661 gtttcctgga gacgaatgct gtgtaaacgg catccggcga tataaggttc aagaatatcc
      721 cagatcactt tgacaaaatt ctctgtactg ggctgtatgt ctctgaaata aggcacatcc
      781 acattcagat tcctgtggtc aaatcgctct actacctccc tcttgatcag cgcactcagt
      841 tccttggcat tcatgatgaa tccggtatcc ggatcaggtt ctccttttac agtcacatag
      901 agatcgtaat tatgaccgtg ccagttcttg ttggcacaac ggccaaatac ctcggcattt
      961 ttctcttctg tccatgcctg gttgtagagg cggtgtgcag cgttgaaatg ctcttttcgc
     1021 gtcagataga ccatattcag gtgcaaaatt aggcgtaatc ggcaaatgcc cgcatatctg
     1081 tgtgtaatgc ttgcagagaa ctgcgcagat tccgcctgca aatactatca ttgtaacatt
     1141 gtactccaac caaactgttt tgctatgatc ctttatgacc aggtatttga aaacaaccaa
     1201 agatgggtag ctgataaact ccgtttgcag ccggaatatt tcagcgagct cagcagcgga
     1261 caaaatcctg atatcctcta cataggttgc agcgatagcc gggtcacagc tgaggaaatg
     1321 acaggtgtag agcccggcca gatgttcgtg caccgcaata tcgccaacct ggtgccaaac
     1381 aatgacctga gctcatccag tgtcatagaa tatgccgtgg cccacctgaa ggtcaaacac
     1441 atcgtggtgt gcggacatta ctattgcgga ggggtcaaag ctgccatgca aagtgcagat
     1501 cttggtatcc tcaatccatg gctgcgtaac attcgcgatg tgtatcgcct gcacaagagc
     1561 gaattgaatg ccatcgcaga tgaaggagag cgctacaaca gacttattga gctgaatgta
     1621 gaagaacagt gcatcaacgt gatcaaaatg gccacctggc aaaagagctt cctcaccacc
     1681 ggcgggccac aggtgcacgg ctggatattc gatgtacaca gcggtcagct caaagacctt
     1741 caactggacc tggaggaaaa actgagtgac atccgctcca tctacgatct gggcaccacc
     1801 aacaacatct gatatgctcg tttacaaagg acttaatctg cgcaccctgc tggtgtggac
     1861 aggctttcat tatggctggc tgtttatcgg tgcggctatc attgccgcat tttatcattt
     1921 cggacatata cgttttctca taccctggct gccggtatcg gtcatcggta cggctgtcgc
     1981 tttctatgtg ggtttcaaga acaaccaggc ctacgatcgt atgtgggagg cacgcaagat
     2041 ctggggtgcc atcgtcaaca gcagtcgtgc ctggggtatg tatgtggatg gattcgtgac
     2101 cgatcagttt agcgaaaagc ggatctctga tatggaacta aaggctgtca agaaaagatt
     2161 gatctatcgc cacatcggct ggctgtatgc attgcgaagt cagctactgc ttacagcacc
     2221 ctgggagcac agcaaccagc gggggccggt aggcagacgt gcccggtatt accagaaaag
     2281 atttggtgtt ggattagtgg atgatgaggt gacccggagc gaattgatga attatcttcc
     2341 agccgatgaa tatgaacggc tcatcaaaca cagcaacacc gcaacccaga tcatcaatga
     2401 gcaatcacgt gatctgactg agcttcgaaa gcaggaactg atcgaagatt tccggcacat
     2461 ggaactacag cagatcctct atcatttcta tgagcaccag gggaaatgtg aacgcatcaa
     2521 gaagtttccc attccacgcc agtatgccaa catgagccgg tatttcgtgg gcattttcat
     2581 catactgctg ccattcagca tgataccgga actgatgcag ttaggcgact ggggcctgtg
     2641 gttgtcagtg cccatcacag cgctgatcgg gtgggtctat ctggtcatgg aactcctggg
     2701 cgattattct gaatttccct tccagggcat ggccaatgac atacccatgt tatcgatttg
     2761 ccgtactatt gagatcgacc tgcgggaaat gctgaatgat gaagacatac ctcctcccgt
     2821 ccaggcagtc gggggcatac tgatgtaggc aaccggaagg agcagcttcc ttgtgccatc
     2881 cactttcccg ggtcccgctt tccgcttgta gttgccgttt gtgatgcgtg cgggtaagca
     2941 aagagccatt tcacaacggc aagctaccgc tgcaatcggg gctatgaagg cccggcagcg
     3001 gcttcgctga gcctgttgga gtggattttt gtctgcatcg ggtttgtact ccttaggcac
     3061 tgatctttta actgaatatt ggcttatccc tgttaccttt ccagagtatt tttggtaaca
     3121 ctgaatgtag gatatctaat gcacgggatg gagctcgacg atctactcat tcaaggcgca
     3181 gattacgctt ataccataca tggatggctg aagggcgtga attcaggggc tcttaatacc
     3241 agtcgagata tgggaggtga tgctaaatca accggtatca gaaagctgat gggtacagat
     3301 gcatttggct tcatactgca ctattatgat ggagactaca ctcaaatcgg ttcggggaac
     3361 tcctttattc cgactgcttc aaccagtggc acaggataca atctagtcac agaaaaccta
     3421 tataatggca atattcgggc tatgactact gccctgatgc gtgaaacgca tgcaaaggtg
     3481 gatgttgtag gtgcagctta tacatatgac caactaaacc gcctgaccgg caggaagacc
     3541 ttcataaagt ctaatatgca cctttccgga aatttcacat ggtctgccat ctctgaatca
     3601 ccgaaatgga gttctgcata tacatatgac ggtaatggta acctactaac tttggaacgg
     3661 gctggtgata atgctacatc ttcgtatgat atggacgacc tcacctacaa ttattatcca
     3721 aatactaacc aattaggaag ggtagatgat gctatatcga gtagtgctta ttcggatgat
     3781 atagatgatc agactggagc aaactactct tacgatgaaa ttggcaatct gatatcggat
     3841 gaccagggag atattgatga cattcaatgg aatgtacagg gtaagataac tttcatcgat
     3901 aaagggagtg gtccggatct aacttttgcc tatgatgcca tgggtaatcg tgttatgaag
     3961 atggtagacg atggaactga gcaggtatat acatactacg tcagagatgc tcagggaaac
     4021 atcatgacaa cctattccag aatagataat gggtctactg acagcatttt cctgggtgag
     4081 caacatattt atggctccgg tagacttggg tatctatcta cccggattag tatggattct
     4141 gttttgccca caacaggata ttactatagg caacttggtc ttcgaagata tgagctatca
     4201 aatcatctgg gaaatgtgct tgtagtgata acagaccgaa ggttactaat tgaagggact
     4261 ggtggattgg cgggtaaaat tgtatcagcg gaacctgatg ttttgcaggc aaatgattat
     4321 tatccctttg ggatgatgat gtcaggaagg acctgg
//
LOCUS       DAOMAX010000224         4332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3662_length_4332_cov_3.636194, whole genome shotgun sequence.
ACCESSION   DAOMAX010000224 DAOMAX010000000
VERSION     DAOMAX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_4332_cov_3.636194"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2556)
                     /locus_tag="PK742_10445"
     CDS             complement(<1..2556)
                     /locus_tag="PK742_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77052.1"
                     /translation="MRPNTWIKHLLFVLPMLISLQAWATHNRAGEITYLYQGDLTFEV
                     TVTTYTDPNSPADRNELIVYYGDGTFDTVSRSDITPMTTDIQVNKYVSTHQYNAAGTY
                     TIYMQDPNRVEGITNIEGSVNVPFYLETTICIPDELLFGYNNSVQLLNPPIDYANIGE
                     LFTHNPGAYDPDGDALRYSLQVPLQAPGLEVPGYEFPDEATACADIFSIDSLTGTITW
                     DVPCEVGIYVIRILIEEFRDGQCVGKVNRDMQIEVVDNPNHAPDVQAVNDTCIIAGET
                     LSLVVTATDEDIGQTITLTAAGGPFAVDVSPAVFDSDPSEGIVEGFFEWNTTCEHIRN
                     EFYTVVFKAEDDFGFGPSSEPLSDQEAWVIYVLAPPPENVVAEAQGNSILVTWDSLYS
                     CFNTENFLGFTIWRKIGCDTLDFDECQRGLEGYGYDSIAFVDTAHRYRDFAVVHGQVY
                     SYRVTAYFGERSEAAPIFTYNEVHGYPSNNGCAELKRDLPIINHVSIRNTDTENGSLL
                     LQWYNPTAEALDTIQNPAPYEYVIKRSAGFTVGGSSEIVQAFTYDFFTEIADTSIIDT
                     LLNTVDNAYAYTIEFYSDGFLLGSAEDASSVYLTGSPADNRSVLTWNFDVPWENFYYT
                     IFKETSAGSGVFDSLSSVTEPLYVDSNLANGTEYCYKVTAYGRYTAESLPQDTLVNLS
                     QYVCVIPEDKEPPCAPELAVHNICEDDQVSFDPADLKNELSWTNPNLTCADDVIGYYI
                     YYSQLQGGQLVFLDSVLLAESTTYTHNNLESLAGCYAVAAVDSFYNVSELSNTVCVDN
                     CSDYNLPNVFTPNNDGANDLYTPILPIRFIAEVDMKIFNRWGALVFETSDPMLN"
     gene            complement(2643..3389)
                     /locus_tag="PK742_10450"
     CDS             complement(2643..3389)
                     /locus_tag="PK742_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQU77053.1"
                     /translation="MNFKLRIWRQADSKSNGKLVEYSISDVNPDMSFLEMMDVLNDRL
                     VKEGQEPVAFDHDCREGICGMCSMYINGRAHGPMKLSTTCQLYMRNFEDGETITIEPF
                     RSAAFPVIKDLSVDRTAFDRIIAAGGYVGVSTGQAQDANAIPIEKHHADMAMDAAACI
                     GCGACVAACKNSSAMLFVSAKVSHLALLPQGQAERKTRVLNMVEQMDEEGFGSCTNTY
                     ACEAECPKGISVTNIARLNREYIKASFSGD"
     gene            complement(3400..>4332)
                     /locus_tag="PK742_10455"
     CDS             complement(3400..>4332)
                     /locus_tag="PK742_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQU77054.1"
                     /translation="PRDISSRSAKEACDDGRGVGKTGLAVYLDFADAIKRLGRDVIEA
                     RYGNLFEMYHHITNEDPYEVPMRIYPAVHYTMGGIWVDYELMTSVPGLYALGEANFSD
                     HGANRLGASALMQGLADGYFVIPYTIGSYLSGEIFTQAIPTDHPAFEEAEKNVRDRIS
                     TLLSINGKRSVDDFHKELGKIMWDHCGMARNAEGLTKARTMIRELRDEFWKDVKVTGT
                     ADSFNPDLEKAARVADFLELGELMVLDALNRNESCGGHFRTEYQTEEGEAQRNDEQYT
                     YVAAWQFNGLDNDPTMHKEELIYENVQLAVRSYK"
BASE COUNT         1020 a         1149 c         1089 g         1074 t
ORIGIN      
        1 gttgagcatg gggtcgcttg tttcaaatac cagtgctccc cagcggttga agatcttcat
       61 gtccacctca gcgatgaaac gtatgggcag gatcggtgtg tacagatcgt ttgcaccatc
      121 gttgttcggc gtgaacacat tcggcaggtt gtagtcagaa cagttgtcca cgcaaacggt
      181 attgctcagt tcgctgacgt tgtagaaact atccaccgcc gcaactgcgt aacatcccgc
      241 caaagattcc agattgttat gtgtataggt tgtagattca gccaggagca cactgtccag
      301 aaaaacgagt tgaccgccct gtaactggga gtaatagatg taataaccga tcacatcatc
      361 ggcacaggtc aggttggggt tggtccagga tagttcattc ttgaggtcgg ccggatcaaa
      421 gctcacctga tcatcctcac agatgttatg tacagccagc tcaggagcac agggtggttc
      481 tttgtcttct ggtatcacac agacgtactg cgacaggttc accagtgtat cctgaggaag
      541 agactctgct gtgtatctgc cataggcagt tactttataa caatactccg ttccgttggc
      601 caggttgctg tcaacataca aaggctccgt cacactgctc aggctgtcga acacacctga
      661 acctgcggag gtctccttga agatggtgta gtagaaattc tcccatggca cgtcaaaatt
      721 ccaggtcagt accgaacggt tatcggccgg agaaccggtc agataaacag aagatgcatc
      781 ttctgcgcta cccagcaaaa agccatcaga ataaaactca atggtatagg cataggcatt
      841 atccaccgta ttcaacagcg tatcaatgat ggaggtatct gctatctcag taaaaaaatc
      901 atatgtgaag gcttgcacta tttcagagct tcctcccacc gtaaatcctg cacttctttt
      961 aatgacgtat tcatacggtg ccggattctg tatcgtatcc agcgcttctg ctgtggggtt
     1021 ataccattgc agcaacaggc tgccattctc tgtatcggta ttacgaatgc ttacgtggtt
     1081 gatgatgggc agatcacgtt tgagttctgc acacccgttg ttagaaggat atccgtgcac
     1141 ttcattgtag gtgaagatgg gtgctgcctc actcctttct ccgaaatagg ccgtcacccg
     1201 gtagctgtac acctgaccat gtaccacagc aaagtcgcgg taccgatgag ccgtatctac
     1261 aaaagcaata gagtcataac cgtagccttc cagtccccgc tggcattcat caaaatccag
     1321 tgtatcgcat cctatctttc gccagatggt gaagcccagg aaattctcgg tattgaaaca
     1381 ggaatagaga ctatcccagg taacaagaat gctgttgccc tgtgcttcgg ccaccacatt
     1441 ttccggtggc ggtgccagca catagatgac ccaggcttcc tgatcagaca ggggctcaga
     1501 actgggaccg aacccgaaat catcttctgc cttgaatacc actgtgtaga attcattccg
     1561 gatatgttca caggtggtat tccactcaaa gaatccttct acgatgcctt cgcttggatc
     1621 agaatcaaaa actgccggac tcacatccac tgcaaagggt ccacctgctg cggtcagagt
     1681 tatggtctgc ccgatgtctt cgtcggttgc agtaaccacc aggctcagcg tttcgcctgc
     1741 tataatacag gtatcattga cggcctgtac atccggtgca tggttcggat tgtccaccac
     1801 ttctatctgc atatcgcggt tcacctttcc cacacactgt ccgtcacgga actcctcgat
     1861 caggatgcga atcacataaa tgccaacttc acagggcaca tcccaggtaa tggtgcctgt
     1921 aagcgaatca atggaaaaaa tatcagcaca ggcagtagct tcatcaggaa attcatagcc
     1981 cggcacttcc agtcccggag cctgtaatgg cacctgcagg gaataacgaa gtgcatcacc
     2041 atccggatcg taggcacccg ggttgtgggt gaacagttct cctatattgg catagtcaat
     2101 gggcggattt aaaagctgta ccgaattgtt gtaaccaaac agcagctcat ccggaatgca
     2161 gatggtggtc tcgaggtaaa acggaacatt caccgatcct tcaatattgg taatgccctc
     2221 taccctgttg ggatcctgca tgtagatggt ataggtgccg gcagcattgt actgatgcgt
     2281 gctgacatac ttgttcacct gaatatcggt ggtcatcggg gtgatgtcac tccgtgatac
     2341 ggtatcaaat gtaccatcgc cgtaatagac gatcagttcg tttctgtcgg ccggactgtt
     2401 cggatccgta taggtggtca ccgtcacttc aaaggtcaga tctccctgat agagataggt
     2461 gatctcgccc gcacggttgt gggttgccca tgcctgcagc gaaatgagca tcggcaggac
     2521 gaaaagcaga tgtttgatcc aggtattcgg tctcatgcgg gaatacacct aaaggtaacg
     2581 aatgaagggc gatgacagta catcagccgt caaatatgga acacctgtga cgctgacggg
     2641 gttcaatcgc cgctgaaaga agccttgata tactcccggt tcagcctggc gatattggta
     2701 accgaaatgc ctttcggaca ttccgcttcg caggcatagg tgttggtgca ggagccaaaa
     2761 ccttcttcgt ccatctgctc caccatgttc agcacacggg tcttgcgttc tgcctgtccc
     2821 tggggtaaca atgccagatg cgacactttc gcagagacga agagcatggc agaagagttc
     2881 ttacaagccg ccacacaggc accgcaaccg atacaggctg cagcatccat ggccatatct
     2941 gcatgatgct tttcgatcgg gatggcgttg gcatcctggg cctgtccggt gctcacacct
     3001 acgtaaccac cggccgcgat gatgcggtca aatgccgtac ggtccacact caggtctttg
     3061 atgaccggga aggcagccga acggaaaggt tctatggtga tggtctcgcc gtcttcaaag
     3121 ttgcgcatgt acaactgaca ggtggtgctc agtttcatcg gaccgtgggc acggccgttg
     3181 atgtacatag agcacatacc gcagatgcct tctcgacagt catggtcaaa agccaccggc
     3241 tcctgccctt ccttgacgag gcggtcgttc agcacgtcca tcatttccag aaaagacatg
     3301 tcggggttca catcactgat gctgtattcc accagcttgc cgttgctctt gctgtcagct
     3361 tgtctccaga tccttagctt gaaattcatg atgctctgat tatttgtatg aacgcacagc
     3421 taattgcaca ttctcgtata tcaattcttc tttatgcatg gtcgggtcgt tgtccagtcc
     3481 gttgaactgc caggcggcta cataagtgta ttgctcatca ttccgctgtg cctcgccttc
     3541 ttccgtctgg tattcggtgc ggaaatggcc accgcaactc tcatttctgt tgagggcatc
     3601 cagcaccatc agctcgccca gctcgaggaa atcggccaca cgtgccgctt tctccaggtc
     3661 agggttgaaa ctatcggccg taccggtcac tttcacgtct ttccagaatt cgtcccgcag
     3721 ctcgcggatc atcgtccggg ctttggtcag tccttcggcg ttccgtgcca tcccgcaatg
     3781 atcccacatg atcttaccca gttccttgtg gaagtcatcc acagatcgct tgccgttgat
     3841 ggacaagagg gtgctgatgc ggtcgcggac attcttctcg gcttcctcga acgcgggatg
     3901 atcggtcgga atggcctggg tgaagatctc tccgctcagg taagaaccga tggtgtacgg
     3961 aatgacgaaa taaccatccg ccagtccctg catcagagca gacgccccga ggcgattggc
     4021 accatggtca gagaagttgg cctctcccag tgcatacagc ccgggcacgg aggtcatgag
     4081 ttcatagtcc acccagatac cgcccatcgt gtagtgcacc gcgggataga tgcgcatagg
     4141 cacttcatac ggatcttcgt tggtgatgtg gtggtacatc tcgaagaggt tgccataacg
     4201 tgcttcgatc acatcgcgac caaggcgctt gatggcatcg gcaaagtcca gataaactgc
     4261 cagtcctgtt ttacccacac cccgtccgtc gtcgcaggcc tctttggccg agcggctgga
     4321 gatatcgcgg gg
//
LOCUS       DAOMAX010000225         4319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3686_length_4319_cov_4.816839, whole genome shotgun sequence.
ACCESSION   DAOMAX010000225 DAOMAX010000000
VERSION     DAOMAX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3686_length_4319_cov_4.816839"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PK742_10460"
     CDS             complement(<1..523)
                     /locus_tag="PK742_10460"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQU77055.1"
                     /translation="MGNIYFASDFHLGVPGHGQSLEREQRIVRWLDSIAPHADRVYLV
                     GDLFDFWFEYRDVVPRGHVRLLGTLARMKDSGIDVQVFTGNHDMWMFGYFEKELGIPV
                     HHDIIRFEVNGKKFLVGHGDGIGPGDKGYKFIKKVFTNKVAQKLFGALHPRIAFGMAQ
                     YWSGKSRNSHEDEG"
     gene            complement(527..1174)
                     /locus_tag="PK742_10465"
     CDS             complement(527..1174)
                     /locus_tag="PK742_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HQU77056.1"
                     /translation="MSRNVSSKELMLTNIRNALINKSKQPFPNLEGTEQVYSREEESL
                     EVLFAQELKAVSGQFVFCETGAELVEILKEVVSIRELKHALCPEPWIQELLEKNGFSD
                     FLKDGAVTGMDAGIMACDALIARTGSILISSRLASGRTLPVYPPVNIVIATTEQLVYD
                     IQDGFDLLRKKYEGTIPSMINLATGPSRTADIEKTLVLGAHGPREVFVFLLDQTS"
     gene            complement(1212..3134)
                     /locus_tag="PK742_10470"
     CDS             complement(1212..3134)
                     /locus_tag="PK742_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77057.1"
                     /translation="MFVSSPLFKGSADAQIPYQWSKSLLPGTYHTSVTDLDTDASGNV
                     FVCGTYKGAVDFDPSAGTYYLTSVGTLVNQFLAKYDSDGNFEWAFSLANSSAVINSSF
                     LSVRVDNAGDVYVSGSLVDQIDFDPGPGTYILDADNGFGFLAKYDTDGNFLWAMVLEG
                     NGISKIYEVAFDDANNVYCAGAFTNSTDFDPGPGTFSLSSNGNNDLFLSKYDEDGNFL
                     IAGAAGGSGVDIAYTIDLDDDNNIYMGGVFQDTVDFDLTPGTDYEMATGVYHGFVAKY
                     NSSGALHFVLNLGDGTQTEVKTLVTSGSNRFYVGGIFMGDVDFDPLGAGDVISNDSMF
                     FHAFVSAYDNYGLQQYTIPLHSDFANCIDLALDASGMVYAVGNFYYSMDFDPYGAGSV
                     TTPVLYDGYLTSYDHYGNHIFKCILTGSSNQNMSAVDINSTDEVHLSGYYNSTVDFDF
                     GAGFAYGFGGLSYQSAFIAKYGQPTILAQQSIQLNGLYDDGQDHLTWTCPEQQEGQFQ
                     IQTFLQGEWVTISDMQATTGTEMYSYYYKPEPACTDESRFYRIVLSRTNGTDLQSNPV
                     SVNSPLSVQCYPSPVNTVLTLIGLPDDMQVILADQTGIPVLETTDHTIDVAWLPAGIY
                     FVLNKQGEVLSRFVHE"
     gene            complement(3616..>4319)
                     /locus_tag="PK742_10475"
     CDS             complement(3616..>4319)
                     /locus_tag="PK742_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQU77058.1"
                     /translation="FNDAIDRVIGGLEKKNKLISPDEKKIIAYHEAGHAICGWFLEHA
                     DPLVKVSIVPRGVAALGYAQYMPKEQFLYTTEQLNDMMCMTLGGRAAEEIVFGRISTG
                     AQNDLERITKMAYAMVTIYGMNKRIGNVSFNDPQQDYRFAKPYSEELAKAIDEEVRSI
                     IDAAYIRTRNLLTDKRSQLESVAQELLQKEIIFKTDMERLIGNRPFEEHKMHEDLTEV
                     EVVEEATDESEKVEP"
BASE COUNT         1082 a         1114 c         1015 g         1108 t
ORIGIN      
        1 agccttcatc ttcatgacta ttacggcttt ttcccgacca atattgtgcc ataccgaatg
       61 caatgcgtgg atgcagtgcc ccgaataact tctgcgccac cttattggtg aataccttct
      121 tgatgaattt ataacctttg tcgcccggac cgataccatc gccgtggccc accaggaatt
      181 tcttgccgtt cacctcaaag cggatgatgt catgatgcac cggtatgccc agctcttttt
      241 caaagtatcc gaacatccac atgtcgtggt tgccggtaaa tacctgtacg tcgatgccgc
      301 tgtctttcat tctggcaagt gtgcccagca accgcacatg acctcttggt accacatcac
      361 ggtattcgaa ccagaagtca aacaggtcgc ctaccagata caccctgtca gcatgcggag
      421 cgatgctgtc cagccagcga acgatgcgct gctctctctc caggctttgc ccgtgaccgg
      481 gaacgcccag gtgaaaatca gatgcaaagt agatattacc caagcctcag gatgtttggt
      541 ccagcaaaaa gacgaacact tctctggggc cgtgggcacc cagcacgagg gtcttttcaa
      601 tatcggccgt gcggctcgga ccggtggcca ggttgatcat ggaaggaatg gtgccttcat
      661 attttttgcg cagcagatcg aatccgtcct ggatgtcata gaccagttgc tctgtagtag
      721 caatgacgat gttgaccggc ggatacaccg gtaaagtacg gccactggcc agtctggagg
      781 aaatgagtat gctgccggtt ctggcgatga gggcatcgca ggccatgata ccggcatcca
      841 taccggttac cgcaccgtcc ttcagaaaat cgctgaaccc gttcttctcc agcagctcct
      901 gtatccaggg ttccggacaa agcgcatgct tcagctcgcg gatactcacc acctctttca
      961 gtatctccac cagttctgct cctgtttcgc aaaagacaaa ctgaccgctc accgctttta
     1021 actcttgggc gaacaatacc tccagcgatt cctcttcgcg gctgtacacc tgctcggtgc
     1081 cttccaggtt ggggaagggc tgcttcgatt tgttgatcag agcgttgcgg atgttcgtga
     1141 gcatgagttc cttgctcgac acgttgcgtg acatgcccta aaggtaatgt gttcagcaga
     1201 atgccggaat actattcatg cacaaagcgg ctcagcacct cgccctgttt attcaggaca
     1261 aaatagatgc ctgcaggcag ccaggccaca tcgatggtat ggtctgttgt ttccaggaca
     1321 ggaatacccg tctgatcagc aaggatgacc tgcatatcat caggcagtcc gatcagggtg
     1381 agcacagtgt tcacgggtga aggatagcac tgcacggaca gcggactgtt cacacttaca
     1441 ggattagact gcagatcagt gccattggtt ctggacagga ctattcgata gaatctggat
     1501 tcatcagtac aggcaggttc cggcttgtag taataggaat acatttctgt tcctgtggta
     1561 gcttgcatgt cggagatcgt gacccactca ccctgtaaaa aagtctggat ctggaattgc
     1621 ccttcctgct gttcaggaca ggtccaggtc aggtgatcct gcccatcatc atagagtccg
     1681 ttcagctgta tggactgctg tgccagtatg gttggctgac cgtatttggc gatgaatgca
     1741 ctctggtagg agagtccgcc aaatccatat gcaaacccgg caccaaagtc aaagtcaacc
     1801 gtactgttat agtaacccga cagatgcacc tcatctgtgg aattgatatc tactgccgac
     1861 atgttctggt tgctggaacc ggtcaggatg catttaaaaa tgtgattgcc ataatgatcg
     1921 taggatgtaa gataaccgtc gtacagcact ggcgttgtaa cagaaccagc tccataggga
     1981 tcaaaatcca tggagtaata aaaatttccc actgcatata ccatgccgct ggcatccagg
     2041 gccagatcga tacagtttgc aaaatcgctg tgcagcggaa tggtgtactg ttgcaaaccg
     2101 tagttgtcgt aggcgcttac aaatgcatga aagaacatac tgtcattgct gatgacatcg
     2161 ccggcaccca gagggtcgaa gtccacatct cccatgaaga tgccacccac atagaacctg
     2221 tttgagccac tggtaaccag cgttttcact tccgtctgtg ttccgtcgcc caggttcagc
     2281 acaaaatgca gggcgccgga tgaattgtat ttggccacaa atccgtgata gacacctgtt
     2341 gccatttcat aatctgtacc gggagtcagg tcaaagtcaa ctgtatcctg aaacacaccg
     2401 cccatataga tgttgttgtc atcatccagg tctatggtat aggcgatatc caccccgctt
     2461 ccgcctgcag cccctgctat caggaaattg ccgtcttcat catacttgct taagaacaga
     2521 tcgttgttac catttgaaga aagactaaag gttcccggtc ccggatcaaa atcggtagaa
     2581 ttggtaaagg cacctgcgca atagacattg ttggcatcat caaatgctac ttcgtagatc
     2641 ttgctgatgc cattcccttc cagcaccatg gcccaaagga aatttccatc cgtatcgtac
     2701 ttggctaaaa atccgaatcc attgtcggca tccagaatat aggtaccagg gcccggatca
     2761 aaatctatct gatcaaccag gcttccggaa acatacacat ctccggcatt gtcaacacgc
     2821 acagacaaga aggaactatt gatcacagca ctgctgttgg ccagactaaa tgcccactca
     2881 aaattgccat cactgtcata tttagccagg aactgattga ccagggtgcc aactgatgtc
     2941 agataatagg tgccggcact cggatcgaaa tcaacagctc ctttgtaagt gccacataca
     3001 aacacattgc ctgacgcatc agtatccaga tcggtcacac tggtgtgata agtgccgggt
     3061 agcagggact tggaccattg gtaaggtatc tgggcatcag cagatccttt gaagagcggg
     3121 ctacttacaa acaacagcgc acaaaatgat actataaaac ttctgcaaca catgatcaga
     3181 gaaagtctaa aaatatagca tagaatagaa ttatcatgtt tttttttaga tgcccttttc
     3241 aatggataca agttcagggt atcatggctt catagatctt tgcctaaagt gttgatatac
     3301 aggattatat gttatttata tccatgtgcc aacgaagtaa aaaaacagaa taaatgaata
     3361 cgtaataaaa tgaatatatg atctatacaa ggttcttcat tcagccggaa ttctgaatgg
     3421 ccgatgtgat cagaaccggc aacgatcttt gccttacaga ggcagaccta ttaaaaaaag
     3481 gagttgacgg gcttttgttt ctgtcagcaa ataattcttt gcaaaacctc acccggcaat
     3541 acttcactgt tggcgcagcc aaaccccctt atgcggggct tactgctcga ctgctttagc
     3601 taactgcaaa ccggctcacg gctctacctt ctcgctttcg tctgttgcct cttcaaccac
     3661 ttccacttcg gtcaggtctt cgtgcatctt atgctcttcg aaaggtctgt tgcctatgag
     3721 gcgctccata tcggtcttga agatgatctc tttctgaagc agttcctggg ctacagactc
     3781 cagctgtgaa cgcttgtcgg tcaacaggtt cctggtgcgt atataggctg catcaatgat
     3841 gctgcgtact tcttcatcaa tggccttggc cagctcttcc gaatacggct tggcgaaacg
     3901 gtagtcctgt tgtggatcat tgaacgatac attgccgatg cgcttgttca taccgtagat
     3961 cgtcaccatg gcataggcca tcttggtgat gcgctccagg tcgttctgcg caccggtgct
     4021 gatgcgaccg aacacgatct cttctgcggc acgaccaccc agggtcatgc acatcatatc
     4081 attcagctgc tcggtggtgt acaggaattg ttcttttggc atgtactgcg cataacccag
     4141 cgctgccact ccacgcggta cgatgctcac cttcaccagc ggatcggcgt gttcgaggaa
     4201 ccagccgcag atggcatgac ctgcttcgtg ataggcgatg atcttcttct cgtcaggaga
     4261 gatgagtttg ttctttttct ccagtccgcc gatgacccgg tcaatggcat cgttgaagt
//
LOCUS       DAOMAX010000226         4314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3695_length_4314_cov_4.513266, whole genome shotgun sequence.
ACCESSION   DAOMAX010000226 DAOMAX010000000
VERSION     DAOMAX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_4314_cov_4.513266"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..738
                     /locus_tag="PK742_10480"
     CDS             316..738
                     /locus_tag="PK742_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77059.1"
                     /translation="MEHRDIIADQIEQMGKVLAYILHDFLRLSGDVPVTQAMEETDHR
                     LQNELDLDPEKVIGLPEEQLMSYLSSRLKADSQLEQLADFLLQTGMTVAPHDQNEAMR
                     RLQRALEIYHTLELQTRTTSLDILAKKKKISEWLSESA"
     gene            717..1451
                     /locus_tag="PK742_10485"
     CDS             717..1451
                     /locus_tag="PK742_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77060.1"
                     /translation="MAERISVDHPAMMKRKRTYRIGKYTFEFLSIFIAVISAFALNNW
                     NDNRRDRLAENKILTEIYNGLENDLNDVRANSQGHSSGLQAVTFFMDVLANNTAQTDS
                     FLIHYFNLTRDFISIQNTSGYETLQSKGLELIRNDALRTAIISLYEYDYTTLKKLEEE
                     YYELQFQENYFHVINAILSEYLLVNDHKQITGISIPLELDQQEEKLLLTCLWKIQANR
                     MFILRYYTEIETRIIQVRGSIKAELE"
     gene            1573..2814
                     /locus_tag="PK742_10490"
     CDS             1573..2814
                     /locus_tag="PK742_10490"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HQU77061.1"
                     /translation="MILQKLFGLFSVLGMAVVFPASAQNQETKSLVFSADLESFRDLN
                     GLKSALSQVEIVALGENTHGLGEVFKAKTELVKFLHEELDFNIVLLESGFGDAALAWE
                     QIEHLSAPAYTRIFSSNFYYQSAEMEALVEYAQAQHGDLIIQGIDCQPQQDYLIDRMA
                     DIGALIDTAWRRITKQEMRGFSYLYQHEMDQDSISFYARRNRFVQFLDSYDDLLREHE
                     DVLQSLGITSGEIHAIRNSNDQLRNTYETVEMGEMLSWPAAYSFRDAAMLHTVQWYRE
                     QFPGQKIIIWAQNSHVENRPKPDYTVSWMGHGLHNLYQEKYYSIGAVVYSGTDLTYSG
                     TFQFEHNDPDYLAYHLNLSDQERYVLDLRGYSGEDFTGQLLLGMENNGATASFIAKDR
                     FDGLLFIRYSDVPVLLRPVEE"
     gene            2902..4215
                     /locus_tag="PK742_10495"
     CDS             2902..4215
                     /locus_tag="PK742_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77062.1"
                     /translation="MKRTLSVVLTILISAISAGAQNKEQYVVTDDVSRFWEAYDLITA
                     TDDTVLQLHYLDSLYLRPGTEGLTGIRQARNYTPQDYLAAIRLYPAYWSSLRPKTLAA
                     ANIGKELEEGIDQLKELYPGLQPATIYFTIGAFRTPGTTIGSNVLIGAELAMADSSCN
                     ATEFPEEMDYLRTYFQTNPNRHLVFLNVHEYVHTQQQPRVHNILSLTLYEGVAEFVAA
                     LALRTSSPNPQISFGKANAGRIREVFETEMFYPNNRYKWLDGNDPNEFGMRDLGYYVG
                     YQICENYYEQAEDKMQAIKTMIGMDYTNEEAVEDFVRRSNYFSAPLDELYQNFESRRP
                     TVIGIEQFENNSEQVDPDISEITIVFSEPLNGFNTGVDLGDGGQSTFPKGTLNGRHWS
                     DDNRSWTIPVELEPNTFYQIFITNNFRTAESIPLKPYLIQFTTGE"
BASE COUNT         1220 a          915 c         1072 g         1107 t
ORIGIN      
        1 aacggaagcg tcagcagcag ggctgctccg gtgagaaaga ggttctttgt ttgcatgtca
       61 tacagttggt tgatgagtcc gaaaatacaa aattggcaac tcacttatac cggatatctg
      121 catattacat tggatcgtgt ccgttttaca atagctattc tggaggcaat attgattttt
      181 caggagtgga aatatcatat tctcagttaa tggtatgtag ttctatacag cgtgtaattt
      241 gtctggatca ccgggattag gctccttgaa caccaccttt cctgaacaga atgatcgtac
      301 atttaccctt tggaaatgga acaccgcgac atcatagcag atcagattga acaaatgggc
      361 aaagtactgg cgtacatttt gcatgacttt cttcgcctgt ctggtgatgt gccggttaca
      421 caagccatgg aagaaacaga tcatcggctg cagaatgaac tggatctgga tcctgaaaag
      481 gtcatcggcc tgccggaaga gcagctgatg tcttacctgt catcccgcct gaaggcagac
      541 tctcagctgg aacaactggc agattttctg ctgcaaacag gcatgacggt ggcccctcat
      601 gatcagaatg aagccatgcg aagattgcaa cgtgccctcg agatttacca tacccttgaa
      661 ctgcaaacac gtaccacttc cctggacata ctggcgaaga agaagaagat cagcgaatgg
      721 ctgagcgaat cagcgtagat cacccggcca tgatgaagcg caagagaaca tacagaattg
      781 gtaagtacac ctttgagttc ctgtccatat ttattgcggt catttcagcc tttgcactca
      841 acaactggaa tgacaacagg cgtgaccggc ttgctgagaa taagatcctg accgaaatat
      901 acaacggcct ggagaatgac ctgaacgatg tcagagcaaa cagccagggg cactcatccg
      961 gcttacaggc ggtgactttt tttatggatg tactggcaaa taatacagct cagacagact
     1021 cattcctgat ccactatttt aacctgacca gagattttat ttccatacag aatacatccg
     1081 gatatgaaac acttcagtcc aaagggctgg aactgatccg gaatgacgct ttaagaactg
     1141 ccatcatttc tctctatgag tatgattata ctacactgaa gaagttggaa gaagagtact
     1201 atgaattgca gtttcaggaa aactatttcc acgtgatcaa tgctatcctg tctgaatatc
     1261 tgctggtaaa tgaccataaa cagataactg gtatcagcat acctcttgaa ctggatcagc
     1321 aggaagaaaa actgttgctt acctgccttt ggaagataca ggcgaacaga atgtttattt
     1381 tgcggtatta tactgagata gaaaccagga tcattcaggt gagaggatcc attaaagctg
     1441 agctggaatg atcgcctgtg tttcatttta agtgaactac ttagaaatag agcctgttgt
     1501 ttccctcttt aggggtgatt gggtaaatta tagctgaata atatttaata agatagtata
     1561 atatttgcat agatgattct gcaaaagttg tttggcctgt tttcggtact gggtatggcg
     1621 gtcgtttttc cggcatcagc tcagaatcag gaaacaaagt ctctggtgtt cagtgcagat
     1681 ctggaatcgt tccgtgatct gaatggcctg aagagtgctt tgagccaggt ggagatcgtg
     1741 gccctgggtg aaaatacaca tggccttgga gaggtgttca aggctaaaac tgaactggta
     1801 aagtttctgc atgaagaact ggattttaat atcgtcctgc tggagtcggg ctttggtgat
     1861 gcagctctcg catgggagca gatcgagcac ctgagcgcac cagcctatac tcgtatattc
     1921 agttcaaatt tttattatca gtcggcagaa atggaagcac tggtggaata tgctcaggca
     1981 cagcacggcg atctgatcat acagggaatt gattgtcagc ctcagcagga ctacctcatc
     2041 gacagaatgg ccgatatagg cgcactgata gatacggcct ggcggcgaat aacaaagcag
     2101 gagatgcggg gattcagtta tctctaccag catgaaatgg atcaggattc aataagcttc
     2161 tatgccagac gcaacaggtt tgttcagttt cttgattcat acgatgacct ccttagagaa
     2221 catgaagatg tattacagtc acttggaatt acctccggcg aaatacatgc tataagaaat
     2281 tcaaatgacc agctaagaaa tacctacgaa acagttgaaa tgggagagat gctttcctgg
     2341 cctgctgcct acagcttcag agatgccgca atgcttcata cggtgcaatg gtacagagaa
     2401 caattcccgg gtcagaagat cattatctgg gctcagaaca gtcatgtgga gaatcgcccc
     2461 aaaccagact acaccgtgag ctggatgggg catggattac acaacctgta ccaggaaaaa
     2521 tattattcca taggtgccgt ggtgtatagt ggtactgatc tgacgtacag cggaaccttt
     2581 cagtttgaac acaatgaccc tgactatctg gcttatcacc tgaacctatc agatcaagag
     2641 cgatacgtgc tcgatctgcg aggttattca ggagaagatt ttaccggaca gcttctgctg
     2701 ggtatggaga ataatggtgc tactgcttct tttattgcaa aagacagatt tgacggactg
     2761 ctgttcatca gatacagcga tgtgcctgtt ttgctcagac ctgttgaaga gtgatctctt
     2821 ttgttcattg tcatgtcaga caatgccgct gacattcggg agtcgtattt ttgccatcct
     2881 aacatatgta agtagagtaa catgaagaga acgctttctg tggtcctgac catcctcatc
     2941 tctgcgatta gtgcgggagc tcaaaataag gaacagtatg tagtaactga tgatgtttcc
     3001 cggttctggg aagcatacga tctcatcacc gctacagatg atacggttct gcaactacac
     3061 tatctggata gtctctatct caggcctgga acggaaggtc tgacaggtat ccggcaggcc
     3121 aggaattata caccacagga ttatctcgct gctatccgtc tgtacccggc atattggagc
     3181 tcgctccggc caaagaccct tgccgcagcg aatattggca aagagctgga agaaggtatt
     3241 gatcaattga aggaactcta tcccgggttg cagcctgcta ccatttactt taccatagga
     3301 gccttcagaa cacccggcac caccatcgga agtaatgtgc tcattggcgc tgagctggct
     3361 atggccgaca gttcctgcaa tgccaccgag tttccggaag aaatggacta tctgcgaacc
     3421 tacttccaga caaatcccaa ccggcatctc gtgtttttga acgtgcatga atatgtgcat
     3481 acgcaacaac aaccaagggt gcacaatata ttatcattga cattatatga aggggtggct
     3541 gagtttgtgg cggccctcgc tttgcgcacc tcttcaccca atccgcagat cagtttcggc
     3601 aaggcaaatg ccgggcgcat cagagaggta tttgaaaccg agatgtttta tcccaataac
     3661 agatacaaat ggctggatgg gaatgacccc aatgagttcg gtatgcgtga cctggggtac
     3721 tatgtgggtt accagatctg tgaaaactat tatgaacagg cggaagataa gatgcaggct
     3781 ataaaaacca tgatcgggat ggactatacc aatgaggaag cagtggaaga ctttgtaagg
     3841 agatccaact atttctcggc acctctggat gagttgtatc agaactttga atcgcggcga
     3901 ccaacggtca ttggaatcga gcagtttgaa aacaacagtg aacaggtgga tcctgatatc
     3961 agcgagatca ctattgtttt ctcagaacct ttaaatggat tcaataccgg agttgatctg
     4021 ggtgatggcg gacaatcaac cttcccgaaa ggtacactga atggcaggca ctggtctgac
     4081 gacaaccgct cctggaccat cccggttgag ctggaaccca acactttcta tcagatcttt
     4141 atcaccaata attttcgtac ggccgaaagc attccactaa agccttatct gatacaattt
     4201 accaccggag agtgagagag agggagtgag agagtgggag tgggagtggg agtgagagtg
     4261 ggagtgggag tgggagtggg agtgggagag agagtgggag agagagtggg agtg
//
LOCUS       DAOMAX010000227         4295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3720_length_4295_cov_4.369340, whole genome shotgun sequence.
ACCESSION   DAOMAX010000227 DAOMAX010000000
VERSION     DAOMAX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3720_length_4295_cov_4.369340"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..687
                     /locus_tag="PK742_10500"
     CDS             190..687
                     /locus_tag="PK742_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77063.1"
                     /translation="MRNLLIVLTTSLLLAACQERSQEQTTESDPTLIAQYSISDYLMR
                     SLGANSGYEGVSFGALDTAWQSPDMDTANYYMEMSEYYLGQAESALFRSHEKSQMYSD
                     SSIYFSNMAVDYINNYPKIRDGWRMEHTYRSMMRDTLQVFTATFYLNDSLQVTGLIPE
                     DLDPE"
     gene            689..1228
                     /locus_tag="PK742_10505"
     CDS             689..1228
                     /locus_tag="PK742_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77064.1"
                     /translation="MTDQSYSFRLPGFSSALPFLAAILGFFLVFTEVDCNGTTMISMT
                     GYDLVTGDRQDPDTGTKEQEDPNIWAIISLGAAALGLILFGITKRRFRWWSMAAMGLI
                     GLLAMWQLHRDVEQSIHAGLDAKSDGDKVDMDLDIGIHFRIGYWIVLGCFGLATLWNI
                     TMARRSGMLPLPVEEKKSD"
     gene            1265..1960
                     /locus_tag="PK742_10510"
     CDS             1265..1960
                     /locus_tag="PK742_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQU77065.1"
                     /translation="MESNTIGFNPAFGSLSGRIPETLFLKDPESSDLGKRILSGSIDM
                     LFEIGFEQFTFLKLARTLQTSEASVYRYFENKHKLLLYLTTWYWAWMDHRLDLALANR
                     RSAKERLRAAVQILTEQVTEDSTFTYINESQLHRVVIEESAKTYMSKEVDDDNKAGAF
                     KGYKQIVARVSDLILELQPNYRYPHMLVSTLIEGAHHQRFFAEHLPSLTDERQNEDTI
                     TRFFTQMVFQTIG"
     gene            1966..3645
                     /locus_tag="PK742_10515"
     CDS             1966..3645
                     /locus_tag="PK742_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU77066.1"
                     /translation="MNTSFSTTMTPVQRFWGLLKPDRREITQLYVYAIFNGLVGLSLP
                     LGIQAIINLIQGGQMSTSWAVLVAFVIAGIAFGGFLQIMQLRITENLQQKVFTRASFE
                     FAYRIPRFKLEALYKHYAPELMNRFFDAMSVQKGLSKLLLDFSTASLQVLFGLILLSL
                     YHPVFIMFSVILLLLVLALFRLTYRRGLDTSLTESKYKYQIAHWLEELARTNFSFKLA
                     GHTTLPMQRTDDMMEHYLHAREGHFRVLKLQYILMVVFKMVIAAGLLILGGILVMDQQ
                     LNIGQFVAAELIILYVLSSVEKLIMNLSELYDVLTALEKIGQVVDLELEQHKGSASIT
                     NTGKGMQIDIQGLRFRYPEMERNILDNINLQITPGEKVCITGQNGSGKSTLISLLAAL
                     YSNQAGSVAYDGLPAGNIDTESLRDQIGACLTEEKIFQGTLAENIHLGRQNVTLEAVR
                     HAASEMGLDAFVQQLPQGFDTIIDPEGRKLPRSIIQKILLARAICNQPRLLLLEDAFE
                     HIQQEEQEVLIHRLTDSGKPWTLIAASSNPVLARHCDRVIAMKNGMIEQIK"
     gene            3652..>4295
                     /locus_tag="PK742_10520"
     CDS             3652..>4295
                     /locus_tag="PK742_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQU77067.1"
                     /translation="MLNITHNSIQQKVRTGDWQRLAQVENRRSGVSLMRLLAILSVIG
                     LIILFLPWTQNIRAGGQVTALQPDQRPQTLHSVIPGRIEKWFVSEGDLVRKGDTILYI
                     SEVKDEYFDPDLLANTKDQLAAKEQSVTSYMEKVRALDQQFDALLAARELKIQQAENK
                     LRQAGLKIASDSIDLVAAEVQYKIAKDQYVRMQELQQQGLKSLTDLENRKAKMQ"
BASE COUNT         1160 a         1036 c         1089 g         1010 t
ORIGIN      
        1 catcgcccac aggctgagta ccatcatgca tgctgaccgg atatacgtgc tggaacaggg
       61 agaggtgatc gaaaccggta cacacgaaaa actcctggag cagaccgggc tgtattatgc
      121 catgtggcga caacagcagg gcgaacgcag aaaattacat gtggcctgac gaatgcgtat
      181 attcgttgca tgcgcaacct gcttatcgta ctaaccacca gtttgttact tgctgcctgt
      241 caggaaagat cacaagagca gacaacggaa agtgacccga ccctgatcgc tcagtacagc
      301 atcagtgact atctgatgcg gtcgctcgga gccaacagcg ggtatgaagg tgtcagtttt
      361 ggtgcgctgg ataccgcctg gcaatcacct gatatggaca ctgccaatta ctatatggag
      421 atgtctgaat actatctggg gcaggcagaa agtgcgctgt tccgcagcca tgaaaagtcg
      481 cagatgtatt ctgattcttc catctacttc agcaacatgg cagtagatta catcaacaac
      541 tatccgaaga tccgcgatgg ctggcggatg gaacatacct atcgctccat gatgcgtgat
      601 accctgcagg tgttcactgc tacgttctac ctgaacgaca gtttgcaggt gaccggcctg
      661 atacccgaag acttagaccc cgaatgatat gacagaccaa agctattcct tccggcttcc
      721 cggattcagt tctgcattgc cctttctggc ggccatcctg ggatttttcc tggtattcac
      781 tgaagtggac tgcaatggta ccacgatgat ctccatgacc ggttatgacc tggtgacagg
      841 agatcgacag gacccggata ccggtacgaa agagcaggaa gatcccaata tctgggccat
      901 catcagcctg ggtgctgcag ccctcggact gatcctgttc ggcattacca aacggcgttt
      961 tcgctggtgg tcgatggctg cgatgggatt gatcggactc ctggccatgt ggcagttaca
     1021 tcgtgatgtg gaacagagta ttcatgccgg actggatgcc aaatcagatg gcgacaaggt
     1081 agatatggac ctcgacatcg ggatacattt ccgcatcgga tactggatcg tattgggctg
     1141 tttcggcctg gccacccttt ggaatatcac catggcccgt cggtctggta tgcttcccct
     1201 tcctgtcgaa gaaaaaaaat cagattgatc tgaacaattc acagttgata gtgttactat
     1261 caacatggaa agcaatacta tcggattcaa tccagccttc ggatctctgt ccgggcggat
     1321 tccggagacc cttttcctga aagatccgga aagcagtgat ctgggcaaac gcattttgtc
     1381 aggcagcata gatatgctgt ttgagatcgg gtttgagcag ttcactttcc tgaaactggc
     1441 caggacactt caaacctctg aggcttcagt gtaccgttat tttgagaata agcacaagtt
     1501 gctgttatac ctcaccacct ggtactgggc ctggatggat catcggctgg atctggcgct
     1561 ggccaatcgc agatcggcaa aagaacgcct tcgtgcagcg gtgcagatac tgaccgaaca
     1621 ggtaacggaa gacagcacat tcacctacat caatgaatct cagcttcacc gggtggtcat
     1681 tgaagaatct gccaagacct acatgagcaa agaggtagat gatgataata aagccggagc
     1741 attcaaaggg tacaagcaga tagtggccag ggtatcagat ctcattctgg aacttcagcc
     1801 taactatcgc tatccgcata tgctggtaag caccttaatt gaaggggcac atcaccagcg
     1861 gttctttgct gaacatcttc cgtcgctaac agatgagcgg cagaacgaag acaccatcac
     1921 acgattcttt acacaaatgg tatttcaaac gatcggataa tacacatgaa cacttcattt
     1981 tctactacca tgacaccggt acagcgtttc tggggattgc tgaaacctga cagacgagag
     2041 atcacacagt tgtatgtgta tgccatcttt aacggtttgg tggggctttc gttgccattg
     2101 ggaatccagg ccatcatcaa cctgatacag ggcggacaga tgtccacctc ctgggcggta
     2161 ctcgttgcct ttgtcattgc cggtatcgcc tttggcggat tcctgcagat catgcagctg
     2221 cgcatcacgg agaacctgca gcagaaggtc ttcacacggg cttcatttga gttcgcttac
     2281 cggatcccga gattcaaact ggaggccttg tataagcact acgcaccgga gttgatgaac
     2341 cggttcttcg atgccatgtc ggtgcagaaa gggctcagca aactgttgct ggatttttct
     2401 acagcgtcct tgcaagtgct gttcgggctt atcctgctat cgctgtatca ccctgtgttc
     2461 atcatgttca gcgtcattct gttgctgctg gtgctggcac tcttccggct cacctatcgc
     2521 cgtggacttg acacttcact tacagaatcc aaatacaaat accagatagc tcactggctg
     2581 gaagaactgg cacgcaccaa tttcagtttc aaactggcag gacataccac tttgcccatg
     2641 cagcgaacag atgatatgat ggagcactat ctgcatgcac gtgaagggca tttccgggta
     2701 ctgaaactgc agtatatcct gatggtggtg ttcaagatgg tgatcgctgc aggactgctg
     2761 atactgggag gtatcctcgt catggaccag caactcaata tcggccagtt cgtagcagca
     2821 gaactcatta tcttatatgt gctcagttca gtggaaaaac tgatcatgaa cctgtctgaa
     2881 ttgtacgatg tgctaacagc tttggaaaaa ataggacagg tagtggacct tgaacttgaa
     2941 caacacaaag gatctgcttc catcaccaac acaggcaagg gaatgcagat agatattcag
     3001 ggactgcgct ttcgttatcc tgagatggag agaaatattc tggacaatat caatttgcaa
     3061 ataacaccgg gagaaaaggt ctgcataaca ggtcagaacg gaagtggaaa aagcaccctg
     3121 atcagtttac tggcagccct gtattcaaac caggcaggat ctgttgcata cgacggattg
     3181 cctgccggca atatagatac agaaagcctg cgagaccaga taggggcctg tcttaccgaa
     3241 gaaaagatct tccagggtac tctggcagaa aacattcacc tgggtcgcca aaatgtcacc
     3301 ctggaagccg tcagacatgc agcttcagaa atgggactgg atgccttcgt acaacaactg
     3361 ccacagggtt ttgataccat cattgatcct gaaggaagaa agttaccgcg aagcatcatc
     3421 cagaagatac tgctggccag agcgatctgc aaccaaccca gattgctgtt gctggaagat
     3481 gcatttgagc atatacagca ggaagagcag gaggtgctca ttcaccggct tactgattcc
     3541 ggcaaaccct ggacactgat agccgcaagc agcaatcctg ttctggccag acattgcgat
     3601 cgtgtgatcg ctatgaagaa cggaatgata gaacagataa aataatcaga catgctcaat
     3661 atcacacata attcgattca gcaaaaagtt cgtaccggcg actggcaacg acttgcacag
     3721 gtagaaaaca gacgaagcgg agtaagtctg atgcggcttc tggccatcct gtctgttatt
     3781 ggtctcatca tcctgtttct gccctggaca cagaacatcc gggcaggcgg tcaggtaaca
     3841 gcattgcagc cggatcaacg tccgcaaaca ctgcattcgg ttatccccgg cagaattgaa
     3901 aaatggtttg tcagtgaagg tgatctggtc agaaaaggag ataccatcct gtatatatca
     3961 gaagttaagg atgagtattt tgaccctgat ctgctggcca ataccaaaga tcagctggcg
     4021 gcgaaagaac agtctgttac ttcctacatg gaaaaggtca gggcattgga tcaacagttt
     4081 gatgccctgt tagctgcccg tgaactcaag atccagcagg ccgaaaacaa gctcaggcag
     4141 gcgggactga agatcgccag cgacagcatc gacctggttg cggcagaagt gcagtacaag
     4201 atcgcaaagg atcagtatgt gcgtatgcag gaattgcagc agcagggatt gaaatcactg
     4261 accgatctcg agaacaggaa ggcgaagatg cagga
//
LOCUS       DAOMAX010000228         4278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3737_length_4278_cov_4.578025, whole genome shotgun sequence.
ACCESSION   DAOMAX010000228 DAOMAX010000000
VERSION     DAOMAX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_4278_cov_4.578025"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..569)
                     /locus_tag="PK742_10525"
     CDS             complement(183..569)
                     /locus_tag="PK742_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77068.1"
                     /translation="MKKTVLSLALITTLLFAACEGGTTYIKTFSNQSNDTITVELLMK
                     DGVDQNYSFPPGTSSDIYWRDWQGSFPGDGYTCVSDIDTMIIDVSGGKTLIKDPFDDS
                     NWELDAQDGRNTERFCTFTVNPDDVQ"
     gene            complement(790..1608)
                     /locus_tag="PK742_10530"
     CDS             complement(790..1608)
                     /locus_tag="PK742_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQU77069.1"
                     /translation="MKKQIILVTGASSGIGKATAFQLLRDGHTVYVAARRLGQMDDLK
                     AKGAIPLSMDITQEEDILKVVARIEQDHGGLDVLVNNAGYAIYGSVEETTIEDARRQF
                     EVNIFGLARLTQLFIPLMRRQQSGTIINITSVGGKIYTPMGAWYHATKHALEGWSDCL
                     RLELKQFGIKVVIVEPGIIKTEFSDVMNQPMIDRSGKGPYAAMVKSVIAAGDRVYQRT
                     FSPPEVIAKTISKAVRSSKPKTRYAAGALAKPMIFLRRWLSDGMFDRMVMSMSK"
     gene            complement(1657..2184)
                     /locus_tag="PK742_10535"
     CDS             complement(1657..2184)
                     /locus_tag="PK742_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQU77070.1"
                     /translation="MTEKQENILRAALQLFAAEGYHATPTSKVAKAAGVSEGLIFRHF
                     GSKEGLLQGILDAGEEAGRKEIGLLLAEKDPESLLRMAIHRPFQVAEDAGQLEFWKLI
                     YKLKYELGRTGEYGRDQLEKALASSFRKLGYAHPQKEASFFLLHFDAVVAALVTGTLE
                     HPRSVRNYWLAKYGL"
     gene            complement(2263..2490)
                     /locus_tag="PK742_10540"
     CDS             complement(2263..2490)
                     /locus_tag="PK742_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77071.1"
                     /translation="MKNGVCPKCQSTEIYTDARQPKRGDRCSLPVTSWTKVFVDLYIC
                     LRCGFFEEYIRPEDLKNEKMMEKVRQQFRKL"
     gene            complement(2536..3375)
                     /locus_tag="PK742_10545"
     CDS             complement(2536..3375)
                     /locus_tag="PK742_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite family transporter"
                     /protein_id="HQU77072.1"
                     /translation="MIDNLLPDEMARMAERIGEKKAHMTAMRMFVLSVLAGSFIAFGA
                     IFATTVTAGSTLPFGITKLVGGVVFSLGLILVIVGGAELFTGNNLIVMAWASKKVSTR
                     LLLRNWMIVYAGNLAGSMCMVAMILLSGYHLGGHGQTGVNMVSIAVQKCSHEPVQAFV
                     LGILCNILVCLAVWLSFSAATTTGKILSVIFPISAFVAAGFEHCVANMYFIPAGMAVA
                     GSGTLDAMLADAGLQLSQLTVQRALLYNLLPVTLGNIVGGAGLVGLVYWFVYLRGNKE
                     VVQ"
     gene            3565..4080
                     /locus_tag="PK742_10550"
     CDS             3565..4080
                     /locus_tag="PK742_10550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HQU77073.1"
                     /translation="MTGAQAQVSEQPHNLFLLVLDVQPAYYTDQEAADIMVRTINSLA
                     ESTPASNMAVTQAAGQSLNISLKGFSKTTLPPAELDPKLQIAQAPVYTKITPNALEQE
                     ELNSQLRELKVNMLLLSGLMAEQCVYHTAIGALKEGYSVYLIADAVMGSTPKKKEKAL
                     KKLAKKGVIIY"
     gene            complement(4089..>4278)
                     /locus_tag="PK742_10555"
     CDS             complement(4089..>4278)
                     /locus_tag="PK742_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HQU77074.1"
                     /translation="IGSVGKPKDGDPRACYVLLEIDEDGLNDVQFRRVAYDVEASAAA
                     VEASVLPQAFADALRTAR"
BASE COUNT         1055 a         1083 c          998 g         1142 t
ORIGIN      
        1 gtacttctgc atctgaaatg atgcgcagat cgatatccca ctgttgtttg tctatttgta
       61 atcccacacc cgtccagtgt ccttccatac catccgtatc cggaactccg gtcagctgcg
      121 caaatgaaag tacaggaaca gccaggagca gaaagaacag tgtgtgtcgc ataggaatga
      181 gtttactgaa cgtcgtcagg attcactgtg aacgtgcaaa agcgttcggt atttcttccg
      241 tcctgtgcat ccagttccca gttgctgtcg tcgaacggat ccttgatcag ggtcttgccg
      301 ccactcacgt caatgatcat ggtatctata tctgacacac aggtgtaacc atcgccggga
      361 aaagaaccct gccagtcacg ccagtagata tcgctggaag taccgggagg aaaggaataa
      421 ttctggtcca caccgtcttt catcagcagt tccactgtaa tggtgtcatt gctctggttg
      481 ctgaaggttt tgatataggt ggtgccacct tcgcaggcag cgaacaggag tgtggtgatc
      541 agggcgaggg ataagacagt tttcttcatg atgcgaaaat aacgcctttg gccggaagtt
      601 atttttgccc cttgctacga gtgttggcgg cctgatgttt gggaagaatt aagaagtata
      661 atttcatttg aaaggatctt ccaagatcct gccacagata cagatctgtt ggcagttaaa
      721 tgcccgttgc tataagatac agaccttcag caacgggcaa tctgtcacat gctccttatg
      781 ttctgagtat catttgctca tactcatcac catcctgtca aacataccgt cactcagcca
      841 tcttctcagg aagatcatgg gttttgccaa tgctcctgca gcatagcgtg ttttgggttt
      901 actggatctt acagcttttg aaatggtctt tgctatgacc tcaggcggag agaaggtgcg
      961 ctgataaaca cggtctcctg cggcgatcac actctttacc atagcagcat aggggccctt
     1021 gcctgaacgg tctatcatgg gttgattcat gacatcagag aattctgttt taatgatacc
     1081 gggttctaca atgaccacct tgataccgaa ttgcttcagt tccagtcgga ggcagtcact
     1141 ccatccttcc agggcatgtt tggtggcatg gtaccaggct cccatgggtg tatagatctt
     1201 gccgccaaca ctggtgatgt tgatgatggt gcctgactgt tgcctgcgca tgagtggaat
     1261 gaacagctgg gttagccttg ccaggccaaa gatgtttact tcaaactgcc tgcgggcatc
     1321 ttcaatggtg gtttcttcta ctgaaccata aatggcatag ccggcattgt tgaccagcac
     1381 atccaggcca ccatggtcct gctctatcct ggctaccact ttcaggatgt cttcttcttg
     1441 ggtgatgtcc atagacaatg gtatggcccc tttggctttg aggtcgtcca tctgaccaag
     1501 tcttctggct gccacataga ccgtatggcc atcccggagc aattgaaagg cggtggcctt
     1561 tcctattccg gaagaagcac ctgttacaag aatgatctgc tttttcattt tattatttat
     1621 aagtgattaa ttacttacta tttagggtaa ttttttttac agtccgtatt tagccagcca
     1681 gtagtttctg accgacctcg ggtgttccag cgttcccgtt acaagggcag ccaccaccgc
     1741 atcaaaatgc aggagaaaaa aggatgcttc tttttgcgga tgcgcatatc cgagttttcg
     1801 gaaggatgat gccaatgctt tttcaagttg gtctctgcca tattcgccgg tgcgacctaa
     1861 ctcgtacttc agcttgtaga tcagcttcca gaattccagc tggcccgcat cttccgcaac
     1921 ctgaaacgga cggtggatag ccattctcag caaagactcc ggatcctttt ctgccagcag
     1981 caggcctatc tctttcctgc ctgcttcttc gcccgcatcc agaatgccct gaagcagtcc
     2041 ttccttactg ccaaaatgac gaaatatcag accttcagaa acaccagctg ccttcgctac
     2101 cttactggta ggggtggcat ggtacccttc tgcagcaaat aactgcaagg cggcacggag
     2161 tatgttttcc tgtttttctg tcaccggagt gagtaattac ttacaaaggt aagacgaatt
     2221 tccattctgc caaattttat tggaagggtg tgatcggtcg gcttacaatt tcctgaactg
     2281 ctgcctgacc ttctccatca ttttttcatt cttcagatct tcgggacgga tatactcttc
     2341 aaagaatccg caacgtaggc agatgtacag atcaacaaac actttcgtcc aggaggttac
     2401 gggtaaagag catcggtcgc cgcgtttggg ctgccgtgca tccgtgtaga tctcagtact
     2461 ctggcattta ggacagactc cgttcttcat aacagggttg ggtttggatg atccctgaaa
     2521 ttacacagcc aattgttatt ggaccacttc cttgtttcca cgcaggtaca cgaaccaata
     2581 taccaacccc accagtccgg ccccacccac tatattgcct aaagttaccg gaagcagatt
     2641 gtacaacaat gcacgttgca cggtcagctg tgaaagttgg aggcctgcat ctgccagcat
     2701 cgcatccagg gtgccgctgc ccgctactgc catgcctgca ggtatgaagt acatattggc
     2761 cacgcagtgc tcaaagccgg ctgccacaaa ggcgctgatg ggaaaaatga ccgacaggat
     2821 cttgccggtg gtggtggcag cactgaaact cagccagact gccaggcata ccagaatgtt
     2881 gcacaggatg cccagcacaa aggcctgaac aggttcatgt gaacacttct gaacagcaat
     2941 ggaaaccatg ttgacccccg tttgtccatg acctcccaga tggtatccgg aaagcaggat
     3001 catcgccacc atacacatac ttcctgccag atttccggca tatacgatca tccagttccg
     3061 caataacaac cgggtgctca cctttttact ggcccaggcc ataacgatca gattatttcc
     3121 ggtaaagagt tcggctccgc ctacaataac gagtatcagc ccgaggctga ataccactcc
     3181 tccaacaagt ttggtgatgc cgaatggcag ggtgctgcct gctgtgaccg tcgtggcaaa
     3241 tatggctcca aaggcaatga aagatccggc aagcacactc agaacgaaca tgcgcatggc
     3301 tgtcatatgt gccttttttt cacctatgcg ctccgccatt cgtgccattt catcaggaag
     3361 cagattgtcg atcatgttct gctgtttggt gtaaaattat ctgccataga tatggaaaat
     3421 ggtggctctt gtcatggata aactttgatt tttgttagca gttaggcggt tattggatca
     3481 catgtttgac atgatcctga ttttttatcg catattttta ccggaatgaa atggcctgga
     3541 tgcctctgta ttttctgctt gctgatgacc ggtgctcagg cacaggtgtc agaacagcca
     3601 cacaatctgt ttctgctcgt attggatgtg cagccggctt actatactga tcaggaagct
     3661 gcagatataa tggtcaggac catcaacagt ttggccgagt caactcctgc ttccaatatg
     3721 gctgttacac aggctgccgg acagtcactg aatatttctc tcaagggttt ttctaaaacg
     3781 acccttccgc ctgcagaact tgatcccaaa ctacagattg cacaggcacc cgtgtacacg
     3841 aagatcaccc cgaatgcact ggaacaggaa gaactgaaca gccaactcag ggaactgaaa
     3901 gtgaatatgc ttcttctttc cggactcatg gctgaacagt gcgtgtacca cacagccatc
     3961 ggtgccctga aggaaggcta ttccgtatat ctgatcgcag atgcagtaat gggcagcacc
     4021 ccgaagaaaa aagaaaaggc gctcaaaaaa cttgctaaga agggtgtgat catatattga
     4081 tctgagtact atcgggcagt ccgcaatgca tcggcaaatg cctggggcaa aacggatgct
     4141 tccacggctg cagcagatgc ttccacatca taggctactc ttctgaattg tacatcattc
     4201 aaaccatctt catcgatttc cagtaacaca tagcaggctc tcggatcacc atctttaggc
     4261 ttccccaccg atccgatg
//
LOCUS       DAOMAX010000229         4256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3769_length_4256_cov_4.027851, whole genome shotgun sequence.
ACCESSION   DAOMAX010000229 DAOMAX010000000
VERSION     DAOMAX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_4256_cov_4.027851"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..813
                     /locus_tag="PK742_10560"
     CDS             211..813
                     /locus_tag="PK742_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77075.1"
                     /translation="MTLPFGMRLYLMIFLIGMAGMTLGQATYFLTGGAAAGVTENNYA
                     ELKLPAAYEGRFIYSNNLGILRFRYNIGASFVQDVWAGHPSVTAGISGVFMKPSGFIK
                     AQTAEDVTYITNKWYLSVDINSVHAAIPVDDSWVYQWGIEPAVVIGRPISKKVLFILS
                     PSYRLNYSPAYVSYGEVTSWSGLFLHLGLLYSPGPRYYKP"
     gene            complement(801..2108)
                     /locus_tag="PK742_10565"
     CDS             complement(801..2108)
                     /locus_tag="PK742_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77076.1"
                     /translation="MREKPRLSFWQIWNMSFGFLGIQFGWGLQMGNMSAIYEFLGASA
                     DEIPFLWLAAPMTGLIVQPIIGFLSDRTWHPRFGRRKPYFLIGAILASATLCIMPYSS
                     TIWMAAGFLWILDASINISMEPTRAFIADMLPKEQLSRGYAFQSFFIGLGAVLAAIMP
                     ILLINVFGMPKTSADGGIPLYVRISFIAGGICFLAAVLYTVLSTKEYPPEYFDEEQGS
                     SQGYLKGLATAFRHMPRSFVRLAPVQFATWLGLFLMWFYLTITITEHVFGATDPNSEQ
                     YADGLAWANLCFGYYSVVTFLFALFMPRLAVMMGNVRLHALCLLAGGIGLLSIALVHD
                     KYVLLLSMTGVGIAWASIVSMPYAIIARDIPAKQMGIFMGLFNMFIVIPEVIAALGFG
                     WVMRELLDNNKLHAVMTGGCFLLLAAILTLFAREKTSGADQGL"
     gene            2207..3652
                     /locus_tag="PK742_10570"
     CDS             2207..3652
                     /locus_tag="PK742_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77077.1"
                     /translation="MLSIQQKLSNSFYALLSLPATAMGFALSIQISALSWLLSTKYGL
                     AIDEIGLVWAAGPIAGIFGQVIFGVWSDNTWFWGGRRRPFIVLGGILAALSLLALPNL
                     DIISRDLGIEGILGVAIAVALALDLSVNVGFNPTRSIIADVTPEGVERTKGYTWMQTI
                     SGTFGMLAYVIGAVMGKYFLIYFGAGLVLLFTVIPPFFIKEPRDFEETKKDGNGFMEG
                     FMAIKPLWGFLIYAIYRIPVRIMEIEVSHHYMEIICGILTIYFIIDSLRLKETGKSHA
                     EAGLIGFKKVLAAHSFTWIGVQTMFIYMFAFLQYRLPDLNNDQVGSVIDISFLILNGV
                     AAVLPAFILEPITRNIGRVRTHLMCIAIMAVGYLGLFFLGSTPTIIYICMAVLGIGWA
                     ATVSLPFAIMSHKVDKKKMGLFMGLFNLSVVLPQLVASLGVGEYLSGAEDKRITFLIC
                     AVCLGISALAWSMVKEQKPDEVAMDMTKSGH"
     gene            complement(3931..>4256)
                     /locus_tag="PK742_10575"
     CDS             complement(3931..>4256)
                     /locus_tag="PK742_10575"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQU77078.1"
                     /translation="TGSHDNDTSKGWFKTAKAADKQYCLDYCKGTQRTIHWDLMRTAW
                     ASVANTAIAPMQDVLGLGTNARMNLPGKADGNWQWRMKEEDLTGALAAKLAAMTKTYG
                     RIVGG"
BASE COUNT         1065 a         1119 c         1001 g         1071 t
ORIGIN      
        1 tggaaacact caatcccctc atgtctctcc ggcccccgag ccggagtctc catctaattg
       61 gcagattatc cagcattttg caaactacct ctacacttaa gatcaatccg gataactatc
      121 gtaattggtt cttcctactt cgaaacaaaa ccaaagagca cgaggtaccg caaatcatgt
      181 tctggaatat tcataccaca accacgcaaa atgacgttac ctttcggcat gcgtttgtat
      241 ctgatgatat tcctgatcgg aatggccggt atgaccctgg gtcaggccac ttacttcctg
      301 accggtggtg ctgcagccgg cgtaacggag aacaactatg cagagctgaa actgccagca
      361 gcctatgaag gtcgcttcat ctattccaac aacctgggta tcctccgttt ccgctataat
      421 atcggggcat cctttgtgca ggatgtatgg gctggacacc cttctgtcac ggccggcatc
      481 tccggtgtct tcatgaaacc ctcgggtttt atcaaggcgc agacagccga agatgtcacc
      541 tacatcacca acaaatggta cctgagtgtg gacatcaact ctgttcacgc agccatcccg
      601 gtagatgact cctgggtcta tcagtggggt attgaacctg ccgtagtaat tggccgtccc
      661 attagcaaga aagtgctatt cattctgtct ccatcctatc ggctgaacta cagtcctgcc
      721 tatgtatcct atggtgaggt gacctcctgg tcggggttat tcctccatct agggttgctg
      781 tatagtcctg gtccgcgata ctataagccc tgatcagcgc ctgaagtttt ttctctggca
      841 aacaatgtaa gaattgccgc caagagaaga aaacatccgc cggtcatgac cgcatgaagt
      901 ttgttgttgt ccagcagttc tctcatgacc cagccaaatc ccagcgctgc aataacctcc
      961 gggatcacaa tgaacatatt gaacaacccc ataaaaatac ccatttgttt tgccggaata
     1021 tctcttgcaa tgatcgcata aggcatagat acaatgcttg cccaggcaat tccaacacct
     1081 gtcatcgata gcaacaaaac atatttatcg tgtaccagag caatactaag caacccaata
     1141 cctccggcaa gtaagcagag tgcatgcagt cttacgtttc ccatcatgac cgccagcctg
     1201 ggcatgaaaa gagcgaacaa aaaggtaaca acggaatagt acccaaagca cagattggcc
     1261 caggccagcc catctgcata ctgctcacta ttcggatctg tggccccaaa aacatgttct
     1321 gttatcgtta ttgtcagata gaaccacatc agaaaaaggc caagccatgt agcaaactgg
     1381 accggggcaa gacggacaaa tgaccttggc atatgtctga atgcggtagc cagtcctttt
     1441 agatatccct ggctggatcc ttgctcctca tcaaaatatt ccggcggata ttcttttgtt
     1501 gacagaaccg tatacagaac agcagccagg aagcagatac ctccggcgat aaagctgatc
     1561 ctcacataca gcggtatgcc accatctgca gaggtcttcg gcatcccaaa tacattgatc
     1621 agtagtatgg gcatgatggc agccaacact gcaccaagtc cgataaaaaa actctgaaac
     1681 gcatatcccc tagacaattg ttctttggga agcatatcag caataaaggc cctggttggt
     1741 tccatactta tattgataga ggcatccagg atccatagaa atccggcagc catccaaata
     1801 gttgatgaat acggcataat gcacagcgta gcagaagcca ggatcgcccc tatcaaaaaa
     1861 tatggctttc tccttccgaa ccgtggatgc caggtgcgat cacttagaaa accaatgatc
     1921 ggttgaacga tcagtcctgt cataggagct gccagccaca gaaatggaat ttcatcggcg
     1981 cttgcaccaa gaaattcata aatggcgctc atattcccca tctgtaaacc ccatccgaac
     2041 tgaatgccca gaaagccgaa gctcatattc catatctgcc agaaactcag tcttggtttt
     2101 tcccgcatgc tgcttatttg aggtgtgaaa attagaaaaa ctattacatt gcaaatccga
     2161 tagaattgac ctgatacctc aaggggtcac accaaaacga tcgcacatgc tatccatcca
     2221 gcaaaaactc tccaactcct tttatgcact gttgtccctg ccggcaacgg ccatgggctt
     2281 cgcactcagt attcagatct ccgccctgag ttggctgttg agcacgaaat acggactggc
     2341 catcgatgag atcggactgg tatgggcggc tggtcccatt gcggggatct tcggccaggt
     2401 catcttcggg gtgtggagcg acaatacctg gttctggggt ggcagacgca ggccgttcat
     2461 tgttttgggc ggcatattgg ctgcactgag tttgctggca ttgcccaacc tcgacatcat
     2521 ctccagagac ctgggtattg aaggaatatt aggggtggcc attgcggtgg ctctggccct
     2581 ggacctgagc gtgaacgtgg gcttcaatcc cacccgttcc atcattgccg atgtgacccc
     2641 ggagggtgtg gaacgtacca agggctatac ctggatgcag accatcagcg gcacttttgg
     2701 tatgctggct tatgtgatcg gtgcggtcat gggaaaatac tttctgatct acttcggcgc
     2761 cggtcttgta ttactcttca cggtcattcc gcctttcttc atcaaagaac cgcgagattt
     2821 tgaagagacc aaaaaggacg gcaatggttt catggaagga ttcatggcca tcaaaccgct
     2881 gtggggattt ctgatctatg ccatctaccg cattccggtg cgcatcatgg agatcgaggt
     2941 gagtcatcac tatatggaga tcatctgcgg catcctgacc atctatttta tcatcgattc
     3001 tcttcgcctg aaagaaacgg gcaagtcaca tgcagaagcc ggactcatag gctttaaaaa
     3061 agtactggcg gcgcacagct ttacatggat cggcgtgcag accatgttca tctacatgtt
     3121 cgcctttctg cagtatcgtc tgcccgatct gaacaacgac caggtgggca gcgtgatcga
     3181 tatttccttt ctcattctga atggcgtagc cgcggtgtta cccgccttta ttcttgaacc
     3241 cattaccaga aacatcggcc gggtgcgcac ccatttgatg tgcattgcca tcatggcggt
     3301 gggctacctc ggcttgtttt tcctgggttc cacacctacc atcatctaca tctgcatggc
     3361 ggtactgggt attggttggg cagccacagt gagcctgccg ttcgccatca tgtcgcacaa
     3421 ggtggataag aaaaagatgg ggctgttcat gggactcttc aaccttagcg tggtattacc
     3481 acaactcgta gcaagtctgg gtgtgggtga gtatttaagc ggtgccgaag acaagcgcat
     3541 caccttcctc atctgtgcgg tatgtctggg catcagcgcc cttgcctgga gcatggtgaa
     3601 ggaacagaaa cccgatgaag tggcgatgga tatgacgaag agcggacatt gattcagttg
     3661 gcagtgggcg attggcagtt ggcaagtggt agcgtctcaa attcataata atatacccgg
     3721 acctgtatca attttaacgc agcgtgcacg gccgcttcgt aacacccgct gcgttttgtt
     3781 ttgactatac atgatcagaa aaacattcca tgaatgttgc ccgtgacaat taaaacgaag
     3841 cgacaagaca aaggctcaga ggatcacgtg atccaatagg tagtggctaa gcttcattcc
     3901 gcttttcgcc ttccgaactc cgccttaccc tcaaccgcca actatcctcc catacgtttt
     3961 cgtcatcgcc gccagctttg ctgccagcgc acctgtcaga tcttcttcct tcatacgcca
     4021 ctgccagttg ccatctgctt ttcctggcag gttcatgcgg gcgttggtgc ctaagcctaa
     4081 cacatcctgc atcggtgcga tggctgtatt ggccacagag gcccaggcgg ttcgcatcag
     4141 gtcccagtgg atagtgcgct gcgttccttt acaataatcc aggcagtatt gtttgtctgc
     4201 tgccttcgct gttttgaacc agcccttcga cgtgtcattg tcgtgcgaac cggtat
//
LOCUS       DAOMAX010000230         4240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3796_length_4240_cov_2.736679, whole genome shotgun sequence.
ACCESSION   DAOMAX010000230 DAOMAX010000000
VERSION     DAOMAX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3796_length_4240_cov_2.736679"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..818
                     /locus_tag="PK742_10580"
     CDS             54..818
                     /locus_tag="PK742_10580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQU77079.1"
                     /translation="MPVYVDDMYKDLNEKPLCTIPRVMCWAVDNIELLKSQPNESVDL
                     IYSDILYGTGRNFGDYQDLKPSRSEIEAHYLPRLKEMHRVLKSTGSIYLQMDYRINHW
                     IRCLMDEVFGYDNFKNTIVWCYGAGGGHGKKYFRRTHDDIHFYTKTNQHTFNKQLNQY
                     GSSLQDWWNIKGLQDKSGFFQNDDERQMYQTQKPKALIERIIKASSNEGDVVADYYLG
                     SGTTAVVCKELNRNFIGCDINPKAIEITKARLNAGT"
     gene            complement(1262..1335)
                     /locus_tag="PK742_10585"
     tRNA            complement(1262..1335)
                     /locus_tag="PK742_10585"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1299..1301),aa:Met,seq:cat)
     gene            complement(1478..1834)
                     /locus_tag="PK742_10590"
     CDS             complement(1478..1834)
                     /locus_tag="PK742_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77080.1"
                     /translation="MKKYLSALLLAGVAILVVEQSSAATDIGPPGIHECHDIVVIDLQ
                     KEAMAVSPLYLSETGVAVVLRSTDFIVPIPVAVLPVAKTAEDPISWRLSSYENSYGEN
                     QGYRPTDRTRFSRDNC"
     gene            complement(1882..2130)
                     /locus_tag="PK742_10595"
     CDS             complement(1882..2130)
                     /locus_tag="PK742_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQU77081.1"
                     /translation="MNIYVANLPFSVDELQVEQLFRKYGTVEKTTIIKDKETGKSKGF
                     GFVEMPNEAEANNAILGLHQFTLEGRKLTCRAANNQRN"
     gene            complement(2165..3058)
                     /locus_tag="PK742_10600"
     CDS             complement(2165..3058)
                     /locus_tag="PK742_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77082.1"
                     /translation="MKNLILLLVMVISFTAAEAQTVRKTFNPDKCYKCESGVFYKLFH
                     IRYCQKCDQVTPPEPPETVQALDADTLFFHNETELRDWCTYHGLGSWTVQPPKFNYYE
                     QMSDVVPYVWGKGCTMRQELGLGNDSMFFIPDANYYMVPEGCRGTSCIADNMQFLHEE
                     MYSGKKYVSFRDHSHLQKEQREMSKCFCSEGDLFNVRETVKNPFTGQVVVFYDIYRWS
                     DRNGENIWNENLKLYTVWPELANYRTVEFELDTYDPETECDYTFREYFPGHETMTLYF
                     QGQKNGVTMTIAEVYGAELGR"
     gene            complement(3100..3378)
                     /locus_tag="PK742_10605"
     CDS             complement(3100..3378)
                     /locus_tag="PK742_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HQU77083.1"
                     /translation="MKRLTQTHELKTWSEYFDAVLAGKKTFEVRKNDRDFQEGDILLL
                     NKWDNVKNEYAGSQTAFTITYVLKGGQFGIEEGYCVLGLKPILPAGCR"
     gene            complement(3391..4026)
                     /locus_tag="PK742_10610"
     CDS             complement(3391..4026)
                     /locus_tag="PK742_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HQU77084.1"
                     /translation="MADFNIAHKLTSRVEGGYVNHPNDPGGETIFGIARNYYPNWEGW
                     KAVDWSKGQPGFPNNMENLRELADTFFKKEFWDTIRGDEIQDQDIANEIYDSSVNFGR
                     SRAVKMAQEAVKIGFGADIDVDGKIGPKTIAALNSCTNKPWLYQLMNMIQGKQYLEIV
                     EKQPKKAVFLRGWIQQRVSRGLLMQFVEFVNSQELQGMSEAMIDKFFAKRQ"
BASE COUNT         1165 a          951 c          888 g         1236 t
ORIGIN      
        1 acatccagag catctgcgat gagtatgggg tggaattaaa attgacatga acgatgccag
       61 tatatgtaga tgatatgtac aaggacttaa acgaaaaacc gctatgcact ataccacgtg
      121 ttatgtgctg ggcggttgat aatatcgaac ttcttaaatc gcaaccaaac gaaagcgttg
      181 atttgattta tagcgatatt ctttatggga cgggtagaaa ttttggagat tatcaagact
      241 taaaaccaag tagaagcgag attgaagcac attatttgcc acgattaaaa gaaatgcaca
      301 gagtgttaaa aagtacagga agtatctatt tgcaaatgga ttatcgaatt aaccattgga
      361 ttaggtgctt aatggatgaa gtttttggat atgataattt taaaaatact attgtttggt
      421 gttatggtgc aggcggtgga catggtaaaa aatactttcg gagaacacac gatgacattc
      481 atttttatac aaaaacaaat caacacacat ttaataaaca attaaatcaa tacggaagta
      541 gtttacagga ttggtggaat ataaaaggat tgcaagataa aagtggtttt tttcaaaatg
      601 atgatgaaag acaaatgtat caaactcaaa aacctaaagc actaattgaa cgaattataa
      661 aagcaagttc aaacgaaggt gatgtggtag cagattatta tttaggttca ggaacgactg
      721 ctgttgtttg taaagaacta aaccgaaatt ttataggttg cgacattaac ccaaaggcta
      781 ttgaaataac taaagctcga ttaaatgcag gaacgtagcc ttgcacataa cttgttgcta
      841 tcagctattg tgaaaaaaaa ctaatagaac gctttacttg cttcctgttc acgtcacccg
      901 tcatgggatg tatggcagaa gcagaaagcc tacgatttgc ggtgaaactt taacgagagg
      961 acataaagcg atgccagatg aaaagcggca gaaacgaata caaaggagag gtggtatggc
     1021 tttgtaaaag ctgccgccga tcatggtaca atggacaatt caaactgtta gatatgtagt
     1081 atcttcacat cgcacagccg attggcggtg taaaagggtt ttcaggttgc ccccgttcct
     1141 ctcaggttcg ggggttttct tttgggcagg caacctatgc aatttttgca caagttcaaa
     1201 aaagaaacca gatccgaaga cctggttcca cattactatc ggcatctgac aaccgatggc
     1261 gtagcaggag caggactcga acccgcgacc tctggattat gaatccagcg agctaaccaa
     1321 ctgctccacc ctgcgatgtg gcgatgtctt catgcgacta tcgacttctg atttctgatc
     1381 attagcctcg ccacctactc tcaccgccag tgcaaatata aaaagaccct gcagaagcag
     1441 ggccttttct caacaactat tatgaaaaca cctgtactca acagttgtcc cgactaaacc
     1501 gggttcgatc agttggtcta tatccctggt tctcgccgta gctgttctca taactggata
     1561 atcgccagga aatgggatct tcggcggtct tcgccaccgg cagcactgca acagggatgg
     1621 gtacgatgaa atccgtgctt ctcagcacca ccgctactcc ggtctcgctc aggtaaagcg
     1681 ggctcacggc catcgcttcc ttctggagat cgataacaac gatgtcatgg cattcgtgaa
     1741 tgccgggcgg accaatatcg gtggccgccg agctttgctc tactacgaga atggctacgc
     1801 cagcaagcag taaagcagaa aggtactttt tcataagcct ttttggttta tagatgaaca
     1861 atgattagag atcctaagat actaatttct ttggttgttg gctgctctgc aggtgagctt
     1921 cctcccttcc agagtgaact gatggagtcc caggatggca ttgtttgcct ccgcctcgtt
     1981 gggcatttcc acgaaaccga agcccttact cttcccggtt tccttgtctt tgatgatcgt
     2041 ggtcttttcc acggttccgt acttgcgaaa aagctgctcc acctgcagtt catctactga
     2101 aaaggggagg tttgctacat aaatgttcat aaagcaatta ctatttaaag tgtgaaaaaa
     2161 tcaatcacct acccaactct gccccatata cctctgcgat ggtcatggtc acgccgttct
     2221 tctgaccctg gaagtacagt gtcatggttt catgaccggg gaagtattct cgaaaagtgt
     2281 agtcacattc cgtttcaggg tcataggtat ctaattcaaa ctctaccgtt ctgtagtttg
     2341 ccaactctgg ccacactgta tagagtttaa ggttttcgtt ccagatgttt tcaccgtttc
     2401 ggtctgacca tcggtagatg tcgtagaaca ccacaacttg cccggtgaaa ggattcttta
     2461 ccgtttctct gacattgaac aaatcccctt cagagcagaa gcacttagac atttcccttt
     2521 gctctttctg taaatgggaa tggtctctga aacttacata cttcttgcct gagtacattt
     2581 cctcatgcaa aaactgcata ttatcagcta tgcacgaagt acctctacat ccttccggaa
     2641 ccatgtagta attggcgtca ggaataaaga acatactgtc attgcctaat cccagttctt
     2701 gtctcattgt acacccttta ccccatacgt aaggaactac gtcactcatt tgttcgtagt
     2761 agttgaattt aggcggctgt actgtccagc tacctaaccc gtgataagtg caccagtcac
     2821 gcaactctgt ttcattatga aagaataacg tgtcagcatc taacgcctgc acggtttctg
     2881 gtggttccgg tggtgtgacc tgatcacatt tctgacaata ccggatatga aacagtttgt
     2941 agaacacccc ggactcacac ttgtagcatt tatcgggatt gaaggtcttt ctcacggtct
     3001 gcgcctcggc agctgtgaag gatatgacca tgaccagcaa aaggattaaa tttttcatgc
     3061 tgtgaagtta tagtttgttt gaggaaagtt agctgccatt tagcgacagc cagcgggtaa
     3121 tatcggttta agtcctaaaa cgcagtatcc ttcttcaatt ccaaattgac cgcctttaag
     3181 gacataagta attgtaaatg ctgtttggct tcctgcatat tcgtttttaa cattatccca
     3241 cttgttgagt aaaagaatgt cgccttcctg aaaatctcta tcattttttc ttacttcaaa
     3301 agttttcttc ccagccaaaa cagcatcaaa gtattctgac caagttttta attcgtgtgt
     3361 ttgagttaat cgtttcataa ttaagcgggg ttactgtcgc tttgcaaaga atttgtcaat
     3421 catggcttcg gacataccct gcagctcttg gctattcaca aactccacga actgcatcag
     3481 caatcccctg ctcaccctct gctgtatcca tccccgcagg aaaacggctt tcttcggctg
     3541 cttctccact atctccagat attgtttgcc ttggatcata ttcatcagct gatacagcca
     3601 tggcttattc gtgcagctgt tgagtgctgc gatggtctta ggaccgattt tgccgtccac
     3661 atcgatatca gccccgaagc caatcttaac ggcttcctgt gccatcttta cggctctgga
     3721 ccggccaaag ttcacggagc tgtcatagat ctcgttggct atgtcctgat cttggatttc
     3781 atcccctcgg atggtatccc agaactcttt cttgaagaag gtatctgcca actctctgag
     3841 attctccatg ttattcggga agccaggttg tcctttggac caatccacag ctttccaccc
     3901 ttcccaattc gggtagtagt tcctggcgat gccgaaaata gtttctccac ccggatcatt
     3961 cgggtgatta acgtagccgc cttccactct ggaagtcagc ttgtgtgcaa tgttaaagtc
     4021 tgccattaga gttcatttta ataattacca cgaatttcaa aatcaccaga atggtcgtta
     4081 atccattcta tacattcaag ttcagttccg taaaaacgga cttctctttt atctaaatcg
     4141 taaacgtaat gcgacattat ttcaaagatt tgtcatggtt tacgccgtac aaagtatgcc
     4201 tcgctcggtg gttttgcatc ccagtctttc tttgcttgtt
//
LOCUS       DAOMAX010000231         4238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3799_length_4238_cov_3.512073, whole genome shotgun sequence.
ACCESSION   DAOMAX010000231 DAOMAX010000000
VERSION     DAOMAX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_4238_cov_3.512073"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PK742_10615"
     CDS             <1..788
                     /locus_tag="PK742_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77085.1"
                     /translation="DRNNLIADAVTADEIFLTTTGEYNLEYGLMDINVQSLNQDTDWI
                     RVEHYWVGADTYKEPVTGLHVHTQRYWRILGGYNGQQTSATIRFNGTISTSGGYLENE
                     FMTTSDDSLLLLYRADPQDEWSIYAYYLLDVWGNHTDKRGAFELSQLLPGEYAIGQYD
                     HSIADDQADSDLCALTTGSAEPGNTDWKLFPNPSNGMFYAIIPASLTARVFDPHGRQI
                     GTMKKQDGIFQADLREGTSGLYLVTLEDSDGRFLDAQKVLIIR"
     gene            809..1696
                     /locus_tag="PK742_10620"
     CDS             809..1696
                     /locus_tag="PK742_10620"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HQU77086.1"
                     /translation="MKNITVIGAGTMGNGIAHVFAMNGYAVSLADVSAPALEKALATI
                     GKNLDRMVSKGSIDEAKKNETLANIKTYTDLNDCATHADLVVEAATENVDLKLKIFTE
                     LDQMCKPECILASNTSSISITKIAAATNRADKVIGMHFMNPVPVMKLVEVIRGYATSD
                     SITATIMELSAKLGKAPVEVNDYPGFVANRILMPMINEAIYTLYEGVAGVQEIDTVMK
                     LGMAHPMGPLQLADFIGLDVCLSILRVLHDGFGNPKYAPCPLLINMVTAGYKGVKTGE
                     GFYTYTPGSKDLVVSERFA"
     gene            1709..2635
                     /locus_tag="PK742_10625"
     CDS             1709..2635
                     /locus_tag="PK742_10625"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HQU77087.1"
                     /translation="MTPVFAKAYEPQEVHFHADDCDQRTEPFRVLICTPDHYEIRDVK
                     NVYMKINDQVDLAKARQQWDDLRNVYLGLKQEGIIDDLLEIPGVEGCEDMVFAANQSL
                     PWVMWNGEKVALMSNMKFETRQTEVLYFEDYYKDLGYTITNLQTRGVFEGMGDCIPHP
                     ARKLVYGGYGFRTERMAYDEVCRVIDAPIITLQLVNEHFYHLDTCFLPLNESEVALVP
                     EAFSSESLGVIRKMFEGVYEIPAEEAMHGFSCNAHIIYNSNRKRTAAIIQKGNPVTLN
                     ILQQSIHKVIEVNTSEFMKSGGSVYCMKMMVY"
     gene            2635..3258
                     /gene="bioD"
                     /locus_tag="PK742_10630"
     CDS             2635..3258
                     /gene="bioD"
                     /locus_tag="PK742_10630"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HQU77088.1"
                     /translation="MAGKYFVTGIGTEVGKTMVSAVLCEALEADYWKPVQAGDPDHTD
                     SMKIAELISNKKTIIHPERYKLSEPMSPHAAAQLDKVRISPRDFELPKTENKLIVEGA
                     GGVMVPLNDYYHMASLMDDLHLPVILVSRNYLGSINHTLLSVEVLRYMDIQIAGIIFN
                     GERNHTTESHIEEYTGLQILARIPQVDQWDKKTVADLAKRVREEFPA"
     gene            3255..>4238
                     /gene="bioA"
                     /locus_tag="PK742_10635"
     CDS             3255..>4238
                     /gene="bioA"
                     /locus_tag="PK742_10635"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQU77089.1"
                     /translation="MRKDRHIWYPFAQQKLASEPVFLTHGKGAWLYDDQGNRYLDAIS
                     SWWTNLHGHAHPYLAEAAKKQFDTLEHVIFAGFTHAPAEALAERLLGKIPAMQKVFFS
                     DNGSTSVEVGIKMALQYWDNSGKPKKQLIALEGAYHGDTFGSMSVSGRDAFTRPFRQH
                     LFDTTYLPFPDDDNRTEVLQAMEEACASGEVAAFIFEPLVQGASGMRMYEAEMLDRLL
                     SIARQHEVLCIADEVMTGFHRTGTFLATDQCMHDPDIICLSKGITGGSLPLAVTLCRQ
                     EIYDAFYSDDKMKALWHGHSYTGNPLACAIANASMDLLESVETQTAIHRIIA"
BASE COUNT         1158 a         1019 c         1097 g          964 t
ORIGIN      
        1 tggaccggaa caatctgata gctgatgctg tcactgccga tgaaatattc ctgaccacta
       61 ccggagagta caatctggaa tacggcctga tggatatcaa cgttcagtcg ctgaatcaag
      121 atactgactg gatcagggtg gaacactact gggtgggtgc agatacctat aaggaacctg
      181 ttaccggttt acatgtgcat acacaaaggt actggcgcat actgggtggc tataacggcc
      241 aacaaaccag tgctaccatc cggttcaacg gaacgatcag caccagtggc ggttacctcg
      301 agaatgaatt catgactact tcagacgata gcctcttgtt gttatatcgt gcagatccgc
      361 aggatgaatg gagcatctat gcgtattacc tactggatgt ctggggaaac catactgata
      421 aacggggcgc ttttgaacta agtcagctgc tacccggcga atatgccata ggccagtatg
      481 accacagcat tgcagatgat caagcagaca gtgatctgtg tgccctgaca accggatctg
      541 cagaacccgg aaatacagac tggaaactat ttcccaaccc cagcaacggc atgttttatg
      601 ccatcattcc cgccagcctg accgcccgtg tatttgaccc gcatggcaga cagataggca
      661 ctatgaaaaa acaggatggg atctttcagg ctgacctccg ggaaggcact tccggcctgt
      721 atctggtaac cctggaagat agcgacggac gctttctgga cgcccaaaag gtgctgatta
      781 ttcgctaagt ttgcgacaaa tcagcagcat gaaaaacatt acggtcattg gcgccggaac
      841 catgggaaac ggcattgcac acgtgtttgc aatgaacggg tatgcagtgt cgctggcaga
      901 tgtaagtgcc ccggccctcg aaaaagcact cgctactatc ggaaagaatc tggaccgtat
      961 ggtcagcaaa ggaagcattg atgaggcgaa gaagaacgag acccttgcca atatcaaaac
     1021 ctatacagat ctgaatgatt gtgccaccca cgcagatctg gtggtagaag ccgccactga
     1081 gaatgtggac ctcaaactga agatatttac cgagctggat cagatgtgca aacctgagtg
     1141 catcctggca tctaacacct cttccatatc catcaccaaa atagctgccg ccaccaacag
     1201 agctgataag gtgatcggca tgcatttcat gaatccggtt ccggtcatga aactggtgga
     1261 agtgatccgt ggttatgcca ccagcgacag tatcacggcc actatcatgg aactcagtgc
     1321 caaactggga aaagcgcctg ttgaagtgaa cgactacccc ggctttgtgg ccaaccgcat
     1381 tttgatgcct atgatcaacg aagccatcta taccctctat gaaggtgtgg caggtgtgca
     1441 ggagatcgat actgtcatga aactcggaat ggcacacccg atggggccac tgcaactggc
     1501 tgatttcatc ggactggatg tgtgcctgtc tattcttcgt gtgttacacg atggttttgg
     1561 aaacccgaaa tacgccccct gtccgctgtt gatcaatatg gtcactgcag gatacaaagg
     1621 ggtcaagacc ggagaagggt tctacaccta tacacccggt tccaaagacc tggtggtctc
     1681 agaacgattt gcctgaaaaa gtattcacat gacacccgtt tttgccaaag catatgaacc
     1741 gcaggaagtg cattttcatg ccgacgactg cgaccagaga accgagccat tcagggtttt
     1801 gatctgcacc cctgatcact atgagattcg ggatgtcaag aatgtatata tgaagatcaa
     1861 tgatcaggtt gatcttgcaa aggcgagaca gcaatgggat gatctgcgca atgtctatct
     1921 cggtctgaaa caggaaggca tcattgatga cctcctggaa attcccggtg tggaaggatg
     1981 tgaagacatg gtttttgccg ccaaccagag cctgccctgg gtcatgtgga acggagagaa
     2041 ggtggcgctg atgagcaaca tgaaatttga gaccaggcaa acagaagtac tgtactttga
     2101 agactattac aaggatcttg gctatactat taccaatctg caaacacgtg gtgtctttga
     2161 aggcatgggc gactgcatcc cccacccggc acgtaaactg gtctatggcg gatatggttt
     2221 ccgcaccgaa cgcatggctt atgatgaagt gtgccgagtc attgatgccc cgatcataac
     2281 tctgcaactg gtcaacgaac acttctatca cctggatacc tgttttctgc ccttgaatga
     2341 aagtgaggtg gccctggtac cggaagcatt cagttctgaa agtttaggag tgatccggaa
     2401 gatgtttgaa ggggtctatg agatcccggc ggaagaagct atgcatggat tttcctgcaa
     2461 tgctcacatc atctacaatt ccaacaggaa gcgcacggct gccatcatcc agaaaggcaa
     2521 tcctgttacg ctgaacatac tccagcaaag tattcacaaa gtgatagaag tgaacaccag
     2581 tgaattcatg aaaagtggtg gctccgtcta ttgtatgaag atgatggttt actgatggca
     2641 gggaaatatt ttgttacggg aataggtacg gaagttggca agaccatggt ctcagctgta
     2701 ctgtgtgaag ccctggaagc tgattactgg aaacctgttc aggcaggaga tccggatcat
     2761 actgacagta tgaagatcgc agaactgatc agcaacaaga agaccatcat ccatcctgaa
     2821 cgatataaac tttccgagcc catgtcgccg catgccgctg cgcagctgga caaggtgcgc
     2881 atctctcccc gcgattttga attacctaaa acagaaaata aactgatcgt ggagggggcc
     2941 gggggtgtga tggtaccatt gaatgactac taccatatgg cctcactgat ggacgacctg
     3001 catctgcctg tcattctggt ttcgagaaat tacctgggga gcatcaacca taccttactg
     3061 agtgtagaag tgctgcgcta catggatatt caaattgcag gtatcatctt caacggcgaa
     3121 cggaatcata ctaccgaatc gcatattgaa gaatacaccg gactgcagat actggctcgt
     3181 attccacagg tggaccaatg ggataaaaag accgtggcag accttgccaa acgggtgcgg
     3241 gaggaattcc ctgcatgagg aaggatcgac acatctggta tcctttcgca caacaaaaac
     3301 tggcttcaga accggtattc ctgacacatg gaaagggagc ctggttgtat gatgatcagg
     3361 gaaaccgata cctggatgcc atcagcagct ggtggaccaa ccttcacgga catgcacacc
     3421 cttatctggc tgaagcagca aagaagcaat tcgatacact ggagcatgtg atatttgcag
     3481 gcttcaccca tgcacctgct gaggcactgg cggaacggtt gctggggaag atcccggcca
     3541 tgcagaaggt attcttttca gataatggaa gtacttctgt ggaagtgggc atcaagatgg
     3601 ccctgcagta ctgggataac tcaggcaaac caaagaaaca actcatcgca ctggagggtg
     3661 cctaccacgg cgacaccttt ggcagtatga gtgtgagcgg gcgggatgct tttacacgtc
     3721 ctttcaggca acacttgttc gatacgacct acctcccctt tcccgacgat gacaacagaa
     3781 cggaagtact tcaggcgatg gaagaagcct gtgcctccgg cgaagtagct gcatttatat
     3841 ttgaacccct ggtgcaggga gcatcaggca tgcgcatgta tgaggcggag atgctggacc
     3901 ggttactgtc gatagccaga cagcacgagg tgttgtgcat cgcagatgag gtcatgacag
     3961 gctttcaccg aaccggtacc tttctggcaa cagaccagtg tatgcacgat cccgatatca
     4021 tctgtctgag caagggaatt acgggaggca gcctgccgct ggctgttacg ctttgcagac
     4081 aggagatcta tgatgcattc tactctgacg acaagatgaa agcgctctgg cacgggcaca
     4141 gttatacggg taatcccctc gcctgcgcta ttgccaacgc cagtatggat ctgctggaat
     4201 ctgtcgagac acaaacggcc attcatcgca tcatagca
//
LOCUS       DAOMAX010000232         4203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3857_length_4203_cov_3.628978, whole genome shotgun sequence.
ACCESSION   DAOMAX010000232 DAOMAX010000000
VERSION     DAOMAX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3857_length_4203_cov_3.628978"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..885)
                     /locus_tag="PK742_10640"
     CDS             complement(145..885)
                     /locus_tag="PK742_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU77090.1"
                     /translation="MLLNKKIVVVLPAYNAALTLEQTYREIPFDIVDDVVLVDDASRD
                     DTADVGRKLGIRHVIVHEQNKGYGGNQKSCYNKALELGADIVIMLHPDYQYTPKLITA
                     MASIIAHDLYPVVLGSRILGKGALRGGMPLYKYVFNRMLTFTQNVLMNQKLSEYHTGY
                     RAFSAEVIRAIRIDANSDDFVFDNQMLAQILYKGYEIGEVTCPTKYFDEASSINFRRS
                     TKYGFGVLGTAMAYRLNRWGWIRSAIFS"
     gene            946..2142
                     /locus_tag="PK742_10645"
     CDS             946..2142
                     /locus_tag="PK742_10645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQU77091.1"
                     /translation="MRILLVTKKSPFPSRDGEAIALRNMMDQYLMLGWQVDMLCMITP
                     KHPYRIADQPEQATYNMVRFTEVYADTSPGKWSGLLNLLGKWSYHMQRFDQPAFRQAL
                     AVLLQERNYDVVQLEGLFMLNYLSVIRLKSSARIIYRSHNIEGNIWKRLAMQEAGIWK
                     KWYLRIQADRLLEAERHLQDQVDFILSISVSDVSAYRQYSPKKKIAYLPTGFDFPEPG
                     PPPIRPDIYFIGALDWMPNIQGLRRFLQEAWPVVLETHPDAKLYVAGRHAPVRFEEEL
                     PDGVSYLGEIEDASRFVHDHAICIVPLWAGSGLKIKIVEALATGKPLVTTPVGMEGLP
                     SGLEEFVIVEQDTGKMARRLAGWITDWPVPAEMAGRASQLVRHTLSNEAVQGQLKTIL
                     RQWNLA"
     gene            2127..3317
                     /locus_tag="PK742_10650"
     CDS             2127..3317
                     /locus_tag="PK742_10650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU77092.1"
                     /translation="MEPGLTVAVICFAVAALALVHSYILYPLLLKVLSSSLPDEKMEE
                     PGSWPVVSVLLAVYNEEKVIRAKLESIFSSDYQADKLELVVGSDGSTDRTNEIIEEFI
                     SKGHRIIFRNFGGRTGKSGIINQISPLANGSIFIPTDANIFFEPGMIRKLVTALQHAD
                     TGLVSANIVNTGMQKDGISHQEEAYIRRENLIKYREGRLWGTMMGAFGACYAIRKELF
                     DPIPANFLMEDFYITLRVIEAGYAAKCDLQAIAYEDVSNSWREEFKRKIRISAGNYQN
                     LGVFYKMLWPFWKPAGFSFLSHKVLRWFGPFWLLITLLTSAFLLHTNLFWCLVFWLQV
                     AGWFTPLLDALLSRWKLHTFVLRLLSYFFLMNLALLAGLWKYMKGIRTSAWNPTARNV
                     NTKQ"
     gene            3314..>4203
                     /gene="ribB"
                     /locus_tag="PK742_10655"
     CDS             3314..>4203
                     /gene="ribB"
                     /locus_tag="PK742_10655"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQU77093.1"
                     /translation="MSEFKLHTIEEAIEDIRNGKLVIVVDDQDRENEGDFITAARNVT
                     PEIINFMATHGRGLICAPLIEDRCDELGLDLMVNKNTALHETQFTVSIDLNGHGCTTG
                     ISANDRALTIQALIDPAMKAEDFARPGHIFPLRAKRGGVLRRTGHTEATIDLSRLAGF
                     EPAGVLVEIMNEDGTMARLPDLVKVAEKFDLRIISIKDLVAYRLRTETLIRKEEVVDL
                     PTKYGHFKLHAYTQLTTGEVHMALVKGDVSNGDPVLVRVHSSCATGDVLGSLRCDCGE
                     QLHAAMEMIDRNGSGVLLYM"
BASE COUNT         1086 a         1006 c         1063 g         1048 t
ORIGIN      
        1 ggatctatct gctgcaacat ttcatggaag acgtgctgtt tggcgctttg ctgggaacca
       61 tgaccgctta tttgctcaca ggtatcctcc tgcatcagaa atggatgtgg acctccttat
      121 gggatcagaa tctgctatca ctgatcagga aaaaatagcc gatcgaatcc atccccatcg
      181 gttcagcctg tatgccatcg ccgtacccag cacaccaaaa ccatatttgg tacttctgcg
      241 gaagttgatg gaagatgctt cgtcaaaata tttggtcgga caggtcactt ccccgatctc
      301 ataaccttta tagagaatct gcgccagcat ctggttatca aatacaaagt catctgagtt
      361 ggcatctatt ctgatggccc tgatcacctc tgctgaaaat gcacggtatc cggtatggta
      421 ttcagataac ttctgattca tcagcacatt ctgggtgaag gtcagcatgc ggttgaaaac
      481 gtatttgtac aacggcatac cacctcgcag tgcacccttg cccagtatcc ttgaaccaag
      541 caccaccgga tacaggtcat gagctatgat gctggccatg gcagtgatca gtttaggcgt
      601 gtactggtag tcagggtgca gcatgatcac aatatctgcg cccagctcca gtgctttgtt
      661 ataacagctt ttctggttgc caccatagcc tttgttctgc tcatgaacga tgacatgtct
      721 gatgcccagc ttccggccaa catcagcagt atcatcacgg cttgcatcat ccaccagtac
      781 cacgtcatct acaatgtcaa acggaatttc ccggtaggtt tgctccaggg ttaacgccgc
      841 attgtatgca ggcaacacga ccacgatctt tttattcagc agcatgaggt gcccaaaaat
      901 acgcaaaacc gcctatggcg tgaagtatta cctttgcgtt gcgctatgcg tatcctgctg
      961 gtcaccaaga agagtccctt cccctcccgc gacggggagg ccatcgccct tcgcaatatg
     1021 atggatcaat acctgatgct cggctggcag gttgatatgc tgtgcatgat cacccccaag
     1081 catccctacc gtattgccga ccagccggaa caagctacat ataacatggt tcgctttaca
     1141 gaagtgtatg cagataccag cccgggcaaa tggagtggcc tgctcaacct gctggggaag
     1201 tggtcttatc atatgcagcg gtttgaccaa cctgctttta gacaagcact tgccgtctta
     1261 ttacaggagc gaaactatga tgtggtgcaa ctggaaggat tgttcatgct gaattatctg
     1321 tcagtgatca ggctgaaaag cagtgcccgc ataatataca gaagccataa tatagaaggg
     1381 aacatctgga agcggcttgc catgcaggaa gcaggcattt ggaaaaaatg gtatctccgg
     1441 atacaggctg atagattgct ggaagctgag cggcatctgc aggatcaggt agatttcatt
     1501 ctgtctattt ccgttagtga tgtatctgcc tacagacagt attctccaaa gaaaaagatt
     1561 gcatacctgc caacgggctt tgattttccg gaacccggcc ctccgccaat acgtcctgat
     1621 atctatttca ttggtgcact tgactggatg cccaacatac agggactgcg gcgctttttg
     1681 caggaagcat ggccggttgt gctggaaacg catcctgatg caaagctgta tgtggccggc
     1741 aggcatgctc ctgtcagatt tgaagaagaa ttgcccgatg gtgtttccta tctgggagaa
     1801 atagaagatg catcccgctt tgtacacgac catgctattt gtatcgtacc actgtgggcc
     1861 ggcagcggct taaagatcaa gatcgttgag gcacttgcaa caggcaaacc cctggttacc
     1921 actcccgttg gaatggaagg cttgccttcc ggcctggaag aatttgtgat cgtagaacag
     1981 gataccggta aaatggcccg aaggctagcc ggttggataa ctgactggcc tgttcctgca
     2041 gaaatggccg gccgtgcaag ccagcttgta agacatactt tatctaacga ggccgttcag
     2101 ggccagctta aaacgattct tcgtcaatgg aacctggcct gaccgttgct gttatttgtt
     2161 ttgctgttgc tgccctggca ctggttcaca gttacattct gtacccctta ttactgaagg
     2221 tgctcagcag ctccttgccg gatgaaaaga tggaagaacc gggcagctgg cctgttgtat
     2281 cagttttact ggcagtttac aacgaagaga aggtcatcag agcaaaactg gaaagcattt
     2341 tttcaagtga ttatcaggca gataagctgg agctcgtagt aggaagtgat ggcagtaccg
     2401 ataggacaaa tgagatcata gaagaattta tcagcaaagg acaccggatc atcttcagaa
     2461 attttggggg aagaaccggt aagtcaggta tcatcaacca gatcagcccg ctggcaaacg
     2521 gcagcatttt tattcctacc gatgccaata ttttttttga accgggaatg atcaggaaac
     2581 tggtaactgc tctccaacat gcagataccg gcctcgttag tgccaatatt gtcaataccg
     2641 gtatgcagaa agatggcatt tcgcaccagg aggaagccta catcaggcga gagaacctga
     2701 tcaaataccg ggagggaaga ttatggggta ccatgatggg agcctttggc gcatgttatg
     2761 ccatcagaaa ggagcttttt gaccccattc ctgccaattt tctgatggaa gatttctata
     2821 tcaccttgcg ggtaatagaa gccggatatg ctgcaaaatg tgaccttcag gccatagcat
     2881 atgaagacgt ttccaacagc tggagggaag aattcaaaag aaagatccgg atatctgcag
     2941 gaaactacca gaacctcggg gtgttctata aaatgctctg gccattctgg aagcctgccg
     3001 gattctcttt cttatcgcat aaggtattgc gatggtttgg tccattctgg ctgctgatca
     3061 ccctgcttac cagcgcattt ctcctgcata caaacttatt ctggtgcctg gtgttctggc
     3121 tgcaggtggc cgggtggttc acgccactgc tggatgcgct gctgagcagg tggaagttgc
     3181 atacctttgt attgcgattg ctgtcttact ttttcctgat gaacctggcc ctgctggcag
     3241 gactctggaa atatatgaag ggcatcagaa ccagcgcctg gaacccaact gccaggaatg
     3301 taaatacaaa acaatgagtg aattcaaact gcataccata gaagaggcca ttgaagatat
     3361 cagaaatggc aaactggtca tcgtagtaga tgatcaggac agggagaatg agggcgactt
     3421 tattactgca gccagaaatg taactcctga gatcatcaat ttcatggcca ctcatggccg
     3481 gggtctgatt tgcgcccccc tgatcgaaga ccgctgcgat gagctgggtc tggatctgat
     3541 ggtcaataag aatacagccc tgcatgaaac gcagttcacg gtatccattg atctgaacgg
     3601 acatggatgc acaacaggta tttctgccaa tgacagggca ctgaccatcc aggcattgat
     3661 cgatcctgct atgaaggcag aagattttgc ccggcccgga catattttcc ccctgcgtgc
     3721 caagcgtggc ggagtgctgc gccgcacagg tcatacagag gctaccatag acctgagcag
     3781 acttgcagga tttgagccgg caggtgtact ggtggagatc atgaatgaag atggcaccat
     3841 ggctcgtctg cctgacctgg tcaaggtggc agagaagttc gatctccgga tcatttctat
     3901 aaaagacctg gttgcctatc gcctgcgcac cgaaaccctg atcagaaaag aagaagtggt
     3961 tgatctgcca acaaaatatg gtcatttcaa actgcacgcc tatacccagc tcacaaccgg
     4021 agaagtacac atggccctgg taaaaggcga tgttagcaat ggagatccgg tgctggtcag
     4081 ggtgcactcc agctgtgcca ccggcgatgt gctggggtct cttcgctgtg attgcggcga
     4141 acaactgcat gcggccatgg aaatgataga ccgcaatggc agtggggtac tattatatat
     4201 gca
//
LOCUS       DAOMAX010000233         4190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3875_length_4190_cov_3.653688, whole genome shotgun sequence.
ACCESSION   DAOMAX010000233 DAOMAX010000000
VERSION     DAOMAX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_4190_cov_3.653688"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1392)
                     /locus_tag="PK742_10660"
     CDS             complement(262..1392)
                     /locus_tag="PK742_10660"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HQU77094.1"
                     /translation="MNKELNIKPVKGWGFNFRRPLTIAGPCSAETEEQVHETAKRLSK
                     YPVDVLRAGIWKPRTRPNSFEGVGSVGLKWLKDAGFDNNLPVAVEVANVKHVYEALRA
                     GIDILWIGARTTVNPFAVQEICDALQGVDIPVLVKNPINPDMELWIGAFERLNRAGIT
                     KMAAIHRGFSTHEKTKYRNIPRWNIPIELKRRYPDLNVICDPSHICGNRELLREVAQT
                     ALDIGFDGIHLESHINPDKALSDAKQQVTPEVFGEIIESLVVRENIDQHLEMEDQLDN
                     LRAQIDSMDKYLLELLSQRMDIVREIGYYKLANNLAIVQPSRWNEIIETRRDMCDRKN
                     LTDKFVAELFHAIHKESIHHQTNVMKQAEQDMEKASSDGEKK"
     gene            complement(1470..2270)
                     /locus_tag="PK742_10665"
     CDS             complement(1470..2270)
                     /locus_tag="PK742_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HQU77095.1"
                     /translation="MSRLFYLGPEGSFHHEMALSLSEYNSEVIASSDAEQVCKLVASD
                     IQAYGLLAIENKLAGAVNTHLRYMLESGCHIKGQYWLPVHHHLLALPGINMEDIEVVS
                     SHPMAFNQSRRFLSDKPWKLRETSSTSAAARELLDGLPGAAAIASKAAMKTYGLNCIR
                     NNVEDPGDNATRFVLLQSSQKNDTDTTDLHEMASFYTPASAIPHMLAEAGSLSVRLVH
                     GWDVPQHDLIYTEWIAETSWPEDLTHTFISRQAVFAGKYGHAEIGNIN"
     gene            complement(2275..4047)
                     /locus_tag="PK742_10670"
     CDS             complement(2275..4047)
                     /locus_tag="PK742_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQU77096.1"
                     /translation="MDPIVTVLLIVGFTVVMFIWEKVPIDVTSLIVMALLILSGSVTM
                     KEGISGFSNEAVITILFLYLLGAGLEKTGAVNVIGRYMLRFAGRGKRRGLLAIMVVSG
                     LLSGLLNNTAVVMIFIPVVFRIARFSKESPSKLLMPLSFASIAGGTLTLIGTSTNILV
                     SGISEDAGYGRFGMFEFTMIGGFLFVSFLLYMFFFGVRLIPRRRSTDSLADSYDLKDF
                     ITEVVITPDSPLVGKRLYNSSLTTDLELDIIEIKDRKGITWLPDDYEILEANDVLIVR
                     GSIDDIMLLKTLPGIQFSKSFELEDEDLRSNETTLIEVVVGPNSSVARSTVDEIDFRD
                     LYDAIPLAIRRRGELLGGRLGETELRFGDDLLIEVRKEAYQSLLRSGDFIFTQELEKT
                     DYDEKKVSMALAIVAGVILLAAFNVLHILEGALIGTILMFILKIISIREAYRDVDWRI
                     IFLLASLIPLGIAIENSGAADLLANYLNHQVSRFGPLVIISSLFAITTLVTSFMSNGA
                     TAVLLSPIAISLAEQMGIDPRPLLLTVMFAASTCYLTPLGYQTNIMIYGPGNYQFKDF
                     MKVGGVLTAITMILVTFLIYFFYF"
BASE COUNT         1081 a          993 c         1032 g         1084 t
ORIGIN      
        1 tgaacaggcg gaagctgcag tctggcagga aaaactgggc gatctctttg aaccctacct
       61 gcaggtacag gattatctgc aacacccgat catgcgaatg gaactgccgt atgaagtgga
      121 ggtgtattag ttggtattgg gtattgggta ttgggtattg agtagtgagt agtgggtagt
      181 gagtagtggg tagtgagtag tgagggataa aaaaaggccc cggaattccg gggcccgacc
      241 taagcatgat ctgtgatcat cctatttctt ttcaccatct gaagaggcct tttccatatc
      301 ctgctcggcc tgcttcataa cattggtctg gtggtgaatg gattctttgt gaatggcatg
      361 aaagagttct gccacgaact tatcggtcag gttctttctg tcgcacatat cgcgtcttgt
      421 ttcgatgatc tcattccatc tgctcggttg tacgatggcc aggttgttgg ccagtttgta
      481 gtaaccaatc tcgcgaacga tatccattct ctgactcagt aattcaagca gatatttgtc
      541 catactgtct atctgcgcac gcaggttgtc cagctggtct tccatctcca ggtgctggtc
      601 tatgttctca cgaactacca ggctctcgat gatctcacca aacacttccg gagtgacctg
      661 ttgcttggca tcactcagtg ctttatccgg attgatgtgg ctttccaggt ggataccatc
      721 aaagccgatg tcgagggcgg tctgtgccac ctctctcagt aactcacggt taccgcagat
      781 atgacttggg tcgcagatga cattgagatc gggatagcga cgcttcagtt caataggaat
      841 gttccacctg ggaatgttgc ggtacttggt tttttcatgg gtgctgaatc cgcggtgaat
      901 ggcagccatt ttggtaatac cggcccggtt cagtctctca aaagcaccta tccataattc
      961 catatccgga ttgatgggat tcttcaccag aacgggaata tccactcctt gtaatgcatc
     1021 gcagatctcc tgcactgcaa atggattaac agtggttctg gcgccgatcc acagaatatc
     1081 gataccggca cgaagcgctt catacacatg ctttacgttg gccacttcta cagcaacagg
     1141 cagattgttg tcaaatccgg cgtctttcag ccattttaat cccactgagc caactccttc
     1201 aaagctgttg gggcgggtgc gtggtttcca gatacctgcc cgaagcacgt caacaggata
     1261 tttggataat cttttagctg tttcatgtac ctgttcttcg gtctctgcgc tgcaaggccc
     1321 ggcaatggta agcggcctgc ggaagttgaa gccccagcct ttaacaggtt taatgttcag
     1381 ttccttattc atgggattaa taaaatgtta agcgattgtt caagttgtta tcaaatgcga
     1441 agcaaaaata gacaacaaac tactaaatcc taatttatgt tgccaatttc tgcatgacca
     1501 tatttcccgg caaatactgc ttgccgggaa ataaatgtgt gtgtcaggtc ttcaggccag
     1561 cttgtttcag cgatccattc ggtatatatc aggtcgtgtt ggggaacatc ccacccgtgt
     1621 actaaccgga cgcttaaact gccggcttct gccaacatat gggggatagc agaagctggt
     1681 gtgtagaaag acgccatttc atgcagatcg gttgtatctg tgtcattttt ttgtgacgat
     1741 tgcaataaaa cgaagcgggt agcgttgtct cccggatctt ccacattgtt acggatacag
     1801 ttcaggccat acgttttcat agctgctttc gaagcaatgg ctgctgcacc ggggaggcca
     1861 tccagcagtt ctctggcagc agcagatgtg gaactggttt cccttagttt ccagggcttg
     1921 tctgacagaa atctccggct ttggttgaag gccatcgggt gactgcttac cacttctata
     1981 tcttccatat taataccggg taaagccagc aggtggtggt gaacaggcag ccagtactgc
     2041 ccctttatat ggcagccaga ctccagcata taccttagat gtgtatttac tgcccctgcc
     2101 agcttattct ctatagccag caaaccatag gcttgaatat cagatgcaac cagtttacag
     2161 acctgttctg catcgcttga agcaatgact tctgagttgt attcagacag tgacaatgcc
     2221 atttcatgat ggaagctacc ttccggaccc aaatagaaaa gtctggacat cagatcaaaa
     2281 gtaaaagaag tatatcagga aagtaaccaa tatcatggtg atggcagtaa gtacaccacc
     2341 taccttcata aaatccttga actgatagtt gccggggcca tagatcatga tattggtctg
     2401 gtagcctaat ggtgtgaggt agcaggtgga agcagcgaac atgacggtga gcagaagcgg
     2461 tctgggatcg atccccattt gctctgccag gctgatggca atgggagaaa gcaacacggc
     2521 tgtggctccg tttgacatga aactggtaac cagtgtggtg atggcaaaaa gactggagat
     2581 gataacgagc ggcccgaaac gtgatacctg gtgattgagg tagttggcca gcagatcggc
     2641 ggcaccggaa ttttcaattg caataccaag gggaatgagt gaagccagca ggaaaatgat
     2701 gcgccagtcc acatcacggt atgcttcacg gatgctgatg atctttagta tgaacatcag
     2761 aatggtaccg atgagagcac cctccagaat atgcaggaca ttaaaagcgg ccagcagtat
     2821 gacacctgcc acaatggcca gagccataga caccttcttt tcatcgtagt ctgttttttc
     2881 aagctcctgg gtgaagatga agtcgccgga acgaagcaat gactgatagg cttcctttct
     2941 cacttcgatc agcagatcat cgccaaaacg cagttctgtt tctcccaatc ggccgcccag
     3001 cagttcacct cttcttctga tggccagagg gatggcatca tacagatcgc ggaagtcaat
     3061 ttcatcgacc gtagaacggg ccaccgaaga gttgggtcca accactactt ctatcagtgt
     3121 tgtttcattc gaccgcaggt cttcatcttc cagttcgaaa gatttgctga actgaatacc
     3181 cggcaatgtc ttgagcagca tgatgtcatc gatgcttccc cgcacgatca gcacatcatt
     3241 ggcttccagg atctcataat catcgggcag ccaggtaatg cctttccggt ccttgatctc
     3301 gatgatgtcc agttcaaggt cggtggtcaa ggaactgttg tacagtcttt tccccaccaa
     3361 aggtgaatca ggagtgatga ccacttctgt aatgaaatcc ttcagatcat aactatcggc
     3421 cagactatct gtggagcgcc tgcgtggaat gagccttacg ccaaagaaga acatgtacaa
     3481 caggaaactg acgaacagaa atccacctat catggtaaac tcaaacatgc cgaagcggcc
     3541 gtagcctgcg tcttcagaaa tgccacttac caggatattt gtggaagtgc cgataagggt
     3601 gagggtgcct cctgctatgg atgcaaaaga cagtggcatg agtagtttgg acgggctttc
     3661 tttggaaaat ctggcaatac gaaataccac cggaataaag atcatgacca ctgctgtgtt
     3721 attgagcagc ccactcagca aaccgcttac caccatgatg gccagcaacc cgcggcgttt
     3781 tcctctgccg gcaaaacgca gcatgtaccg cccgatgaca ttgacagcac cggttttttc
     3841 caatccggcc cctaataagt agaggaaaag aatggtgatg accgcctcgt tgctgaaacc
     3901 gctgatgccc tctttcatgg tgaccgagcc actgaggatc agcagggcca tgacgatgag
     3961 agaggtcacg tctatcggga ctttctccca gatgaacatg accaccgtaa accctactat
     4021 aagcagaacg gttacaattg gatccataag tatgatgagc tgcctctaac aaagataaaa
     4081 ggagttatga atgcaggaaa aaatactcga acttgccatc atggccactt atgaccggga
     4141 tctggaagca tggatggaac gcctgcagca agatgcactg gtatataacc
//
LOCUS       DAOMAX010000234         4144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_3926_length_4144_cov_4.026412, whole genome shotgun sequence.
ACCESSION   DAOMAX010000234 DAOMAX010000000
VERSION     DAOMAX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3926_length_4144_cov_4.026412"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PK742_10675"
     CDS             <1..1058
                     /locus_tag="PK742_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU77097.1"
                     /translation="NKFTKVSNLEYPINSGEDDFALLMETRPVGKDTLSMGYFSSGRS
                     GGSGSDDLYQYIRTNKKPRPPVFVLQVRVLRKIYEDSLDVTSKPVDTVYLPGAALSAG
                     LSGAMNILPVFMQGNDPDSIYTAEVNVGETYSLKASMDGFFARSENISTTGWTAAPGD
                     TLVVLYEMVLDQIPTSTTTQIRLNNIYYDFNDTTLRPESFPELDQLVTLLTENPGLTI
                     QINSHTDARGKDSYNQKLSQGRANSVVVYLISKGIAPERLFAKGVGESEPDVIRADVS
                     TPSGVLVPKGTVLTEKFINTFKSNKDDFEFLHQFNRRTTFAVLSDDFRLESETPDDVE
                     VDPAPDDGVRDDTPKED"
     gene            complement(1088..2131)
                     /locus_tag="PK742_10680"
     CDS             complement(1088..2131)
                     /locus_tag="PK742_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HQU77098.1"
                     /translation="MSRQPVEYTEYLQLEKILHAQEPESFKEGTPAHDEMLFIIIHQA
                     YELWFKQIHFELDSAIRVMLQEEVNDNSPALQTIVHRTSRIVTILKVLVQQIDILETM
                     TPLDFHDFRDLLRPASGFQSWQFKMLEARLGLKFDHRYGQEYYLSQLKPQYVDLIKKV
                     EAEPTWLELLERWLVRMPFFADQDLWQHYQSITKESSEHHVFWNDYREVYRSTLSAEE
                     SGNADAFDRIFLGQGEQHTTLSPAAMRAALFIKLYSGYPILQQPFQMIQNMLDIDEQM
                     ATWRFRHINMVHRMIGSRVGTGGSTGKEYLQGALNKHYVFRDFALLSSFLIERKRLPE
                     LSPELQKRLGFWG"
     gene            complement(2171..4102)
                     /locus_tag="PK742_10685"
     CDS             complement(2171..4102)
                     /locus_tag="PK742_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HQU77099.1"
                     /translation="MYKHLQQFIEVLEEHGELLRVKEFVDPRLEITEIADRFIKQNGP
                     ALLFENTGTDFPLLINALGSEKRMCLALGVAHLDDIAKEIETLFHSLSEPKRTLADKV
                     RMLPQLGKIASWMPKTIGGKGACQQVIMQDPDLTRLPVMTCWPSDGGPFITLPVIHTM
                     DPENGIRNVGMYRMQVFGKDLTGMHWHKHKVSAAHFRKYQAMGKKMPVAVILGGDPVY
                     TYAATAPLPPNVDEYMLAGFIRKKKVELVKCITLTEERFGFDIHVPADADIVIEGYVD
                     PADDLIWEGPFGDHTGYYSLADWYPKFHVTCITHRKDAVYPSTIVGIPPQEDAWIGKA
                     TERIFLAPIKMTMLPEMVDMDMPIEGVFHNLTLASVKKEFPGHGQKIMNAMWGAGQMM
                     FNKILVVHSEETDIHDYATVARTISEQVDPWQDIILSQGPADVLDHSCSKFAFGGKMF
                     LDATIKLEEEVNETAKYHTPSEVKIDVSSIQNAYTEVHGLYTGLLNKGISAVLVSVKK
                     DKPGHVKQLHASLRQEAGLDRIRFFIYVDHLVPADDVATVIWHFANNIDPKRDVMLSE
                     HNAQGVSQAGIDGTRKTRALDQFQRPWPNIIVMNDEIIDRVDERWQMLGLGNFISSPS
                     LRYRGQLLPGGAVVEEAAY"
BASE COUNT         1032 a         1055 c          975 g         1082 t
ORIGIN      
        1 gaaacaagtt caccaaagtc agtaatctgg aatatccgat caactccggc gaagatgact
       61 tcgccctcct gatggaaacc agacctgttg gaaaagatac cttgtctatg gggtatttca
      121 gctccggccg ttcgggtggc agtggcagcg acgatctcta tcagtacatc agaaccaaca
      181 agaaacccag accaccggta tttgtgctgc aggtcagggt gctgcgcaag atatatgaag
      241 acagtctgga tgttaccagc aaaccggttg ataccgtgta cctgcctggt gccgcactga
      301 gtgctggact gtcaggtgcc atgaacatct tacctgtctt tatgcagggc aatgatcctg
      361 actctattta taccgccgaa gttaatgtgg gagaaacgta ttcactcaaa gcttcgatgg
      421 atggattctt tgcccggtca gaaaatattt caacaacagg atggaccgct gcacccggcg
      481 ataccctggt ggttctgtat gaaatggtgc tggaccagat acccacctcc accaccacac
      541 agatcaggct gaacaatatt tattacgatt tcaatgatac caccctgagg ccggaaagct
      601 ttcctgaact ggatcagctg gtcaccctgc tgacagaaaa ccccgggctg accatacaga
      661 tcaattctca tactgatgcc aggggtaaag acagttataa ccagaaacta tctcagggca
      721 gggccaatag tgtagtggtc tatctcattt ctaaaggtat tgcgccggaa cgcctgttcg
      781 ccaaaggtgt gggtgagtcc gaaccggatg tgatcagggc agacgtcagt accccttcag
      841 gtgtacttgt accaaaaggc acggtgctga ccgagaaatt catcaacacc ttcaagagca
      901 acaaggatga ttttgagttt ctgcaccagt tcaacagacg cactacgttc gcagtattaa
      961 gcgatgactt ccgtctggaa agtgaaaccc ccgatgatgt ggaagttgac cctgcaccgg
     1021 atgatggtgt aagagatgat accccgaaag aagactgagt taattagcta attaactcat
     1081 tcattcatca gccccagaaa cccagtcgtt tttgcaattc agggctcagt tccggaagcc
     1141 tttttctttc tatcaggaag ctggacagta aggcaaaatc tctgaacaca tagtgcttgt
     1201 tcagggcacc ctgaagatat tctttaccgg tgctgccccc ggttccaacc ctgcttccta
     1261 tcatccggtg caccatattg atgtgtctga atctccaggt ggccatttgt tcatcaatgt
     1321 ccagcatgtt ctggatcatc tgaaaaggtt gctgcaggat cggatagcca ctgtacaact
     1381 tgatgaaaag tgccgcacgc atagctgccg gactgagtgt ggtatgctgc tctccctgac
     1441 caagaaaaat ccggtcaaag gcatctgcat tgcctgactc ttccgcactc agggtggatc
     1501 ggtaaacctc cctgtagtca ttccagaaaa catggtgttc actgctttct ttcgtgatgc
     1561 tttggtagtg ctgccacaaa tcctgatctg caaagaaagg catacgcacc agccagcgtt
     1621 ccaacagttc cagccaggtg ggttctgctt ctactttttt aatgagatct acatattgtg
     1681 gtttcagctg agagagatag tattcctgtc cgtaccgatg gtcaaacttc aatcctaatc
     1741 ttgcttccag catcttgaat tgccagctct gaaatccgga tgccggcctg agcagatcgc
     1801 ggaagtcatg aaaatcaagc ggtgtcatgg tctccagaat atcgatctgc tgcaccagca
     1861 ccttaagaat ggtaacgatg cgggatgttc tgtgtacgat ggtctgcaag gcaggtgagt
     1921 tgtcgttcac ttcttcctgc agcattaccc gaatggcaga gtccagttca aaatgaatct
     1981 gtttgaacca gagttcataa gcctgatgga tgataatgaa caacatctca tcatgggcag
     2041 gagtaccttc cttaaaactt tcaggttcct gcgcatgcag gatcttttcc aactgcaggt
     2101 actcagtata ttcaacgggc tggcgactca tcatctgatt ttgccctaaa agtacggaat
     2161 tggagaaagg tcagtatgcc gcttcctcca ccacagcacc acccggcaac agctgtccgc
     2221 gataacgcag cgatggcgat gaaataaaat tgccaagacc cagcatctgc catcgctcat
     2281 ccacccgatc gatgatctca tcattcatga caatgatatt gggccagggt ctctggaact
     2341 gatccagtgc tctggtcttg cgggttccgt ctattccggc ctgcgatacc ccctgagcat
     2401 tatgctcact gagcatcacg tcacgcttcg gatctatgtt gttggcaaaa tgccagatga
     2461 ccgtggctac atcatcagcc ggcaccagat gatccacata gatgaagaac ctgatcctgt
     2521 caagccctgc ttcctgccgc agggaagcat gcagttgttt cacatgaccc ggcttgtctt
     2581 tcttcacaga tacaagcacc gcagagatgc ctttgttcag caaacctgta tatagtccat
     2641 gtacttcagt atatgcattc tgtatgctgc tcacatctat cttcacctca gatggtgtat
     2701 ggtattttgc tgtttcattg acctcttctt ccagtttgat ggtggcatcc agaaacattt
     2761 taccgccgaa ggcaaacttt gaacagctat ggtccagcac atctgccggg ccctgactga
     2821 ggatgatatc ctgccagggg tccacctgtt cactgatggt tctggctacc gtggcgtaat
     2881 catgaatatc tgtttcttca gaatgcacca ccaggatctt attgaacatc atttgtccgg
     2941 caccccacat ggcattcatg atcttttgtc cgtgaccggg gaattctttt ttgacagaag
     3001 caagtgtcag gttgtgaaac accccttcaa ttggcatgtc catatcaacc atttcaggca
     3061 acatggtcat tttgatcgga gccaggaaga tcctttcagt agccttgcct atccaggcat
     3121 cttcctgcgg cggaatgcca acgatggtgg agggataaac cgcatctttc cggtgggtga
     3181 tgcaggtgac atgaaacttc ggataccagt cggccagaga atagtatcct gtatgatcgc
     3241 cgaacgggcc ttcccagatg agatcatcag cgggatccac atatccttcg atcacgatat
     3301 cagcatcggc cggcacgtga atatcaaaac cgaaccgctc ttccgtcagg gtgatgcact
     3361 tcacaagttc cacctttttc tttcgtatga atcccgccag catatattca tccacattgg
     3421 gaggcaacgg agctgtggcg gcataggtat ataccggatc gccacccaga ataaccgcta
     3481 ccggcatctt ctttcccatg gcctggtatt ttctgaaatg cgccgcactt actttgtgtt
     3541 tgtgccagtg catgccggtc aggtccttac cgaaaacctg catgcggtac atacctacat
     3601 tgcgaattcc attctcggga tccatggtgt ggataacagg cagggtgatg aacggcccac
     3661 cgtctgatgg ccagcaagtc ataacaggca gtctggtcag atcaggatcc tgcatgatca
     3721 cctgctgaca ggcgccttta ccgccgatgg tctttggcat ccaggaagcg atcttaccca
     3781 gctgaggcag cattctgacc ttatcagcca gcgtgcgttt gggttcactg agagagtgga
     3841 ataaggtttc aatctctttg gcaatatcat caagatgtgc cacaccaagt gcaagacaca
     3901 tgcgtttttc actacccagt gcattgatga gcagggggaa atctgttccg gtattctcga
     3961 agagtaatgc cggtccgttt tgtttgatga acctgtctgc gatctctgtg atctccagtc
     4021 tcggatcaac aaattccttt acgcgaagaa gctctccgtg ctcttccagt acctctatga
     4081 attgctgcag gtgtttgtac actgtttgtt ttttcaggta acaaaaatag gcaaagaagg
     4141 ttgg
//
LOCUS       DAOMAX010000235         4078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4031_length_4078_cov_4.507581, whole genome shotgun sequence.
ACCESSION   DAOMAX010000235 DAOMAX010000000
VERSION     DAOMAX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_4078_cov_4.507581"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PK742_10690"
     CDS             complement(<1..275)
                     /locus_tag="PK742_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQU77100.1"
                     /translation="MSFHSGFVNIIGLPNAGKSTLSNALVGERLSIITPKAQTTRRRI
                     IGIVNEPGYQIIFSDTPGIIDQPEYKLHAWMNAQISQALQDADVILY"
     gene            682..1374
                     /locus_tag="PK742_10695"
     CDS             682..1374
                     /locus_tag="PK742_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQU77101.1"
                     /translation="MRYKILLLCFALGGGMAFAQFGEVGGVVTDTITGEPLGFANISL
                     EQGDQIITQSISDENGKYLFSNLNDGIYSIQISYVGYAPARFDNIKVRNKELTVYHLK
                     LHSGSDLPVVFVFPDRIADVPDGRQIIDIKDLRETGARDPGQIVAQSAAGVYAKDGGD
                     GGENGGGLYISGSRQDATLYVVDGIRVIGSTYVPKSAIANITVYTGGIPANYGDFTGG
                     VIEITTRGFAGN"
     gene            1426..1501
                     /locus_tag="PK742_10700"
     tRNA            1426..1501
                     /locus_tag="PK742_10700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1459..1461,aa:Met,seq:cat)
     gene            1540..2850
                     /gene="der"
                     /locus_tag="PK742_10705"
     CDS             1540..2850
                     /gene="der"
                     /locus_tag="PK742_10705"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQU77102.1"
                     /translation="MSFTVAIVGRPNVGKSTLFNRLTGNRQAIVDDLSGVTRDRHYGV
                     VDWNGRSFNIIDTGGFVAHSDDVFEKAIRSQVEIAINEASILLLMVDVTTGLTDLDDD
                     IIDRLRRIQKRVIIVVNKVDNSQRQLQATEFYSIGFEHTHFVSSITGSGTGELLDEVV
                     AMMPADDSSDAEEEEVPRIAIVGQPNVGKSTLLNALIGEERNIVTDIAGTTRDAIHTR
                     YQLFGKDFILIDTAGIRKKKSVREDVEFYTVIRAIKAIDEADVCILMIDAQTGVEGQD
                     LTILQTIVKKKKGLVILVNKWDAVEKDHHTMKYYTEGIKNRIAPFRDVPIVFISALEK
                     TRIFQAVEAALQVMENKKTKVATSVLNETLLPIIEATPPPTVRGNYVKIKYITQLPST
                     TPSFAFFTNYPKDIKDSYRQFLENKLRETFPFSGVPINIFFRQK"
     gene            2963..3175
                     /locus_tag="PK742_10710"
     CDS             2963..3175
                     /locus_tag="PK742_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77103.1"
                     /translation="MKKLSIFAVALALTIFVSCKGKESNDDDMMDEGMQEQVDSNASD
                     DMSDAGNADSEDLSTEEELFAVDLDE"
     gene            complement(3292..3456)
                     /locus_tag="PK742_10715"
     CDS             complement(3292..3456)
                     /locus_tag="PK742_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77104.1"
                     /translation="MEVLITLFVNPGVLFVFTLGISVLLLLGFARLLKRQQNRPLGAD
                     QENLKFTRKK"
     gene            complement(3456..>4078)
                     /locus_tag="PK742_10720"
     CDS             complement(3456..>4078)
                     /locus_tag="PK742_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU77105.1"
                     /translation="GRRSFGKGLVQEVYPLPDTSAVRLTIARYYTPSGKSIQRSYANG
                     TDAYYDEYMQIVMNGDADSVNGRKADWGIAPDIEVALDTSDLSRRFNRLYNLGLVQQF
                     SYGWYGNHKKQMEGYADVQAFQKDFHMSDEMFRSFLNYVSDKEPEQSLPADTASLLRP
                     RIETGIMAVLARQQWGNDGFFPILNSMDPDIQAALDAAYRKEEHKK"
BASE COUNT         1034 a          997 c         1044 g         1003 t
ORIGIN      
        1 atgtataaaa taacatctgc atcctgcagc gcctgtgata tctgtgcatt catccaggca
       61 tgcagcttgt attccggctg gtcgatgatg cccggtgtat cggaaaatat gatctggtag
      121 cccggttcgt tcacgatgcc gatgatgcgc ctgcgggtgg tctgcgcttt gggtgtgatg
      181 atggacaggc gttcacccac cagggcattg ctgagggtgg attttcctgc attcggcagg
      241 ccgatgatgt tgacaaaacc cgagtggaag gacatggggc aaaggtaagg tgcagttggc
      301 agttggcggt tagtcccgat agctatcggg agcagtcggc agtcggcagt cagcagtcag
      361 cagtcagcag tcagcagtga aggattaact gacaaatatc gacagtctgt tgcaatccag
      421 gttggcacat tgcttcatgg aggaaatgtc tccccggacg gggagtgccc gaaggggagg
      481 ggtgatgttc agttgacaga ctcctgtgtt caatattctt tagatcatta attagtcagg
      541 atgtttcaaa aatttgacct tgccaactgc caccttgcca actgccaact gtaaactttc
      601 tacccttccc ttttactttc ccctgacaat tctccctgtg gaatccattc aagacccgca
      661 tctccatata aacgtatgca tatgagatac aaaattctac ttctttgctt cgccctggga
      721 gggggcatgg cattcgctca gttcggtgag gttggcgggg ttgtgaccga taccatcacc
      781 ggcgaaccgc ttgggtttgc caatatctca ctggagcaag gcgaccagat catcacccag
      841 agcatttcag atgaaaatgg taaatacctg ttcagcaatc tgaatgacgg tatctattcc
      901 attcagatca gttatgtggg ttatgcgccg gcccggttcg ataatatcaa ggtgcgaaat
      961 aaggaactga cggtctatca cctgaaactg cattcaggtt ccgatctgcc tgtggtcttt
     1021 gtgtttccgg acaggatcgc agatgttccg gatggcagac agatcattga tatcaaagac
     1081 ctgagggaaa ccggggcacg tgatccggga cagatcgttg ctcaaagtgc agctggtgta
     1141 tatgccaaag atggcggaga tggtggtgag aacggcggcg gactctacat atccggaagt
     1201 cgccaagatg ctactctgta cgtggtggat ggtatccggg tgatcgggtc cacgtatgta
     1261 cctaaaagtg ccatagccaa catcaccgtc tatacaggag gtatcccggc taattacggt
     1321 gatttcaccg gtggcgtcat tgagatcact acccggggat ttgccggaaa ctagttgcgg
     1381 gtttttgata tccgtattat ttgccctatc tttgccgtcc aataccgcgg ggtggagcag
     1441 ttggtagctc gtcgggctca taacccgaag gtcacaggtt cgagtcctgt ccccgctacc
     1501 aaaggggttg acactgagtc aaccccattt ttttaagcca tgagtttcac tgtagccata
     1561 gtaggcagac ccaatgtggg caaaagcacc ctgttcaacc ggctgaccgg caacagacag
     1621 gccattgtgg atgacctgag cggggtgacc cgcgacaggc actatggggt ggtggactgg
     1681 aacgggcgct cctttaacat catcgacacc ggaggttttg tagcacattc tgacgatgta
     1741 ttcgaaaaag ccatccgcag ccaggtggag atcgccatca atgaggccag cattctgttg
     1801 cttatggtgg atgtgaccac aggcctcaca gacctggacg atgacatcat cgaccggttg
     1861 agacgcatcc agaaaagggt catcatcgtc gtcaataaag ttgacaacag ccagcgtcaa
     1921 ctgcaggcta ccgagttcta ttccatcggc tttgagcata cacattttgt ttcctccatc
     1981 accggaagcg gaacaggaga attattggat gaggtagtgg ccatgatgcc ggcagatgat
     2041 tcttctgatg cagaagagga agaggttccc cgcatcgcca tcgttggtca accgaatgta
     2101 ggcaagtcca ccctgctcaa cgccctgatc ggggaagagc ggaacatcgt gacagatatt
     2161 gcaggcacca ccagagatgc catccacacc cgttaccagc tgttcggaaa agactttatt
     2221 cttattgata cagccggtat ccgcaagaag aaatctgtgc gggaagatgt ggagttctat
     2281 acggtcatcc gggccatcaa ggccatcgat gaagcagatg tctgtatcct catgatcgat
     2341 gcgcagaccg gtgtggaagg acaggatctg accattctgc aaaccatcgt caaaaagaaa
     2401 aagggtctgg tcattctggt gaacaaatgg gatgcagtag aaaaggatca ccacaccatg
     2461 aagtattata cggagggtat caaaaatcgc atcgctcctt tccgtgatgt gcccatcgta
     2521 tttatttcag cacttgaaaa gacccggata ttccaggcag tggaagcagc cctgcaggtg
     2581 atggagaaca agaagaccaa ggtggccacc agtgtactga atgaaacatt gctgcccatc
     2641 atcgaggcga cacctccgcc aacagtaagg ggcaactatg tgaagatcaa gtatatcacc
     2701 cagcttccca gcacgacccc gtcttttgct ttctttacga actatccgaa agacataaaa
     2761 gactcttaca gacagttcct ggaaaacaag cttcgggaaa cattcccgtt ctccggggtg
     2821 cctatcaata ttttcttccg ccagaagtaa cagccgtggg gcagttgctg ttctgcagag
     2881 agccttcctc ctgatggcaa tcacgtggaa ccaacctcaa actgccaact gaaatttcta
     2941 tctttatgaa aacacttgac caatgaagaa actgtccatt ttcgctgtag cactcgctct
     3001 gaccattttt gtttcctgca aaggcaaaga aagtaatgat gatgatatga tggatgaggg
     3061 aatgcaggaa caggttgaca gcaatgcttc tgatgatatg tcagatgccg gcaatgctga
     3121 ttctgaggat ctttctacag aagaagaatt gtttgctgtt gatttggatg aatgaccggc
     3181 aacgaatgaa cgaactgcaa gtcccgccca acagcggggc ttttttttgc ttccagttcc
     3241 aacgtcattt caggagaggc atttagctta actcctatac tagcagttca actatttctt
     3301 tcgtgtaaac ttcaggttct cctggtcagc tccaagcggt ctgttctgtt gtcttttcag
     3361 caaacgggca aaacccaata gcagaagcac actgataccc agggtaaaaa cgaacagcac
     3421 ccccgggttg acgaacaggg tgatcaacac ctccatcatt tcttatgctc ttcttttcgg
     3481 tatgcggcat ccagtgcagc ctgaatatcg ggatccatgc tgttgagtat ggggaagaac
     3541 ccatcgttac cccattgctg gcgggccagt acggccatga taccggtctc aatgcgtggg
     3601 cgtagtaagg aagccgtatc agcaggtaac gattgctccg gttccttatc tgacacatag
     3661 ttcaggaagc tgcggaacat ctcatcgctc atgtggaagt ctttctgaaa ggcctgcaca
     3721 tcagcatacc cttccatctg tttcttatga ttgccgtacc agccatagct gaattgctgc
     3781 accagtccca ggttgtacaa acggttgaaa cgtctgctga ggtcagaagt atccagggcc
     3841 acctctatat caggtgcaat gccccagtca gccttacgac cgttcacaga atcggcatca
     3901 ccattcatca cgatctgcat gtattcgtca taataggcgt cggtaccgtt tgcatagctg
     3961 cgctggatgc tcttgccact cggagtatag taacgggcga tggtcagcct gacggcggat
     4021 gtatcaggca gcggataaac ttcctgtacc agtcctttac caaaagacct ccgaccta
//
LOCUS       DAOMAX010000236         4075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4037_length_4075_cov_4.594776, whole genome shotgun sequence.
ACCESSION   DAOMAX010000236 DAOMAX010000000
VERSION     DAOMAX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4037_length_4075_cov_4.594776"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PK742_10725"
     CDS             <1..814
                     /locus_tag="PK742_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77106.1"
                     /translation="DGHWLTGKGAVETKGVIRGDFGRSYTTKLPVSNILRERIGWSLF
                     FTFTSVLLAYLISLPVGLRAAAKPHSLFDRSSSVVLFLLYSMPAFWLATLLLMNFANP
                     DVLKWFPASGVAPAGGFPAGTGWWGRITGTIPYLVLPTICYTYAQLAFLSRMTRVSTL
                     EIYGQDFIRTARAKGLSARTVLYRHAFRNALLPIITVFANVFPAAIGGSVILETVFSI
                     PGMGREIVQAIYQQDYPVIVAVFTLTGVLTLAGYLLADILYSVADPRITWKR"
     gene            802..2130
                     /locus_tag="PK742_10730"
     CDS             802..2130
                     /locus_tag="PK742_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77107.1"
                     /translation="MEKMSNSLSRASWKQFRRNRPAMAALWFLSVMLLLALMAPLLAN
                     DRPLYARYHDKHIFPALYPNKTYIFVLSDGSDEYVKSALVNWKTLPLEKAVWAPVAWW
                     PGESDYANAHFVSPGGSQQFRDASGVLTEMPQKFRHRLGTNNTGEDVAAGLIHGARVS
                     LSIGMIAMGIAACIGLLLGAVAGYFGDKGIHMTRGQWSTLIPGLFIAWFYAFHVRHFA
                     LTDALQAGGWKAVTSLSFSTMLFTAILLGCWWLGKLLSRIPFFQKGSWLPADNLISRT
                     IEIIHSLPVFMLIITIAAITKPSIVSVMILLGLTSWTGIARLTRAEFLRIRELDYMQA
                     GRSLGLGAARLIWRHALPNALAPALVSIAFGVASAILAESALSFLGVGVPTDLVTWGS
                     LVNEGRQQYSAWWLVVFPGLFIFGTVTAYNLIGDGLRDAMDPKLRKQIRI"
     gene            2267..3727
                     /locus_tag="PK742_10735"
     CDS             2267..3727
                     /locus_tag="PK742_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77108.1"
                     /translation="MMRLLFLFVLFSTVQVSAQQIYISEGQKLSSEMVGYDILGKGPA
                     GELFVYKKYRFEDEVDVFDKQMNLKRTRNITLKGINYDTNELIKMGDRIFQFYTERSG
                     RTSYLKFQEYNLALENKGDPVLVDSTVSRIGEAWSEYKVQRSEPDEYILCYKFSLNNG
                     RMEQTYLQILNREGKMVSQSQFYLPAGNDTYELLTTQIGRGGQPAFLFRNNSYNCKKD
                     EDGIHYWIAFPGNGGKLSTCPISNAGDQCIKELNIGFDEARNILVAAGFTGEDNRYSM
                     QGYTVIHIDPLTGLVTSEKNDQFSDELSSSITSMLDKYSRYIPAYQVTRVIPRMDGGA
                     LLVAEYYDKTVENYDYTNYDPYYGYRTSTRQVEFYEYSDILLLSTGPEGKLDWSNIIR
                     KKQLSKEDKGVNSSFGLMNSYRNLYFIFNEDISQNSNVLMYALSADGTLDRQSLFNPS
                     KQEVELRPVAARQIDFDEVIIPSIYKRTLAFVKVKL"
     gene            3732..>4075
                     /locus_tag="PK742_10740"
     CDS             3732..>4075
                     /locus_tag="PK742_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77109.1"
                     /translation="MIRLFRSVTPFSLLAGLLYLAAMSLHLFLHPVVPDLRPLQEPYS
                     DWFPVAQWSGHDLYMIVLLVLLMTAAFMVSVTAAGASFTHGLSPLPAIVYLTLCFLVP
                     DAFIHPVSVITVT"
BASE COUNT          965 a         1041 c         1002 g         1067 t
ORIGIN      
        1 agatggccac tggctaaccg gaaagggcgc cgtcgaaaca aagggggtca tacgaggtga
       61 tttcggcaga tcatacacca ccaaactacc tgtatccaat atactccgtg aacgcatcgg
      121 atggtctttg ttcttcacat tcacgtccgt actgctggcc tacctcatca gccttcctgt
      181 cggattgcgg gctgcagcta agccccattc actgtttgac agaagttcat ctgtggtcct
      241 gttcctgttg tattccatgc ccgccttctg gctggccacc ttgttgctga tgaacttcgc
      301 caatccggat gtgctcaaat ggtttccggc cagtggtgta gcgccggcag gtggttttcc
      361 cgcaggcacc ggctggtggg gaagaattac cggaaccatc ccctatctgg tgctgcccac
      421 catctgttac acctatgcgc aactggcatt tctgagcagg atgacccgtg tatctaccct
      481 cgagatctac ggacaggatt tcattcgcac cgcccgtgca aagggtcttt cagcacggac
      541 agtgctttac agacatgcct ttcgcaatgc cctcctgccc atcatcaccg tatttgccaa
      601 tgtatttcct gcagccattg gcgggtcggt catactggaa accgtattca gcataccagg
      661 aatgggcaga gagatcgtgc aggctatata tcagcaggac tatccggtca ttgtggctgt
      721 gttcacgctg accggtgtgc tgactttagc cggttatctg ctggctgata tattatactc
      781 tgttgcagac ccaaggatca catggaaaag atgagcaaca gcctgagccg tgcttcctgg
      841 aagcaattcc gccgcaacag accagccatg gccgcgttgt ggtttttgtc agtgatgctg
      901 cttcttgcat taatggcccc gctgctggct aatgaccgtc cgctctatgc ccgttatcac
      961 gataaacata tttttcctgc actatatccc aacaaaacat atatatttgt tttatcagac
     1021 ggatctgatg aatatgtcaa atctgccctg gtcaactgga agaccctgcc cctcgaaaaa
     1081 gctgtctggg ctcctgtggc ctggtggccg ggtgagagcg actacgccaa tgcgcatttt
     1141 gtttctccgg gcggtagtca gcaattcaga gacgcttccg gtgttctgac agaaatgcca
     1201 cagaaattcc gccaccggct gggcaccaac aataccggag aagatgtagc tgcaggactg
     1261 atacacggag ccagagtctc tttgagcatt ggcatgatcg ccatgggtat tgcggcctgt
     1321 atcgggttgt tgctgggtgc agttgcaggt tactttggcg acaaaggcat acacatgaca
     1381 cgcgggcagt ggagcaccct aataccggga cttttcatag cctggttcta tgcattccat
     1441 gtgcgccatt ttgcgctgac cgatgccctg caggcaggag gttggaaagc tgtaacttcc
     1501 ctttccttca gcacgatgtt gtttaccgcc atactcttag gatgctggtg gttgggtaaa
     1561 ctgctgagcc ggataccgtt ctttcaaaaa ggcagctggt tgccggcaga taacctcatc
     1621 tcccgcacca tagagatcat acattccctt ccggttttta tgctgatcat caccattgca
     1681 gccattacca aaccatccat tgtcagtgtg atgatcctgt tgggactgac ctcctggacc
     1741 ggcattgcga gacttacacg ggcagagttt ctccggatcc gtgaactgga ttatatgcag
     1801 gcaggacgct cacttggcct gggagctgcg agacttatct ggcggcatgc gctgcccaat
     1861 gcgctggcac ctgcgcttgt aagcatcgcc ttcggagtag cgtcagccat cctggctgaa
     1921 tcagctctgt catttctcgg tgttggtgtg cccactgatt tggtcacatg gggttcactt
     1981 gtcaatgagg gaaggcaaca gtacagtgcc tggtggctgg tggtgtttcc cggcctgttc
     2041 atctttggta ccgtgacagc ctacaatctg atcggtgacg gactgcggga tgccatggat
     2101 ccaaagctgc gaaagcagat ccgtatctga gcatcagccg ttcacatgca tggtaccacc
     2161 acccgtccaa ccattagcag aataccaata acgaaaaaca aatgaccagc aaccctgtac
     2221 ttccatacct tcagacaacc atcttcgtta tatttttgtt gttaccatga tgcgcctttt
     2281 gttcctgttt gtactgtttt caactgtgca ggtttctgcc cagcagatct atatctcaga
     2341 aggtcagaag ctgagcagtg aaatggtggg ctatgatatt ctggggaaag gaccggccgg
     2401 agaactgttt gtgtataaga aataccgttt tgaagatgag gtggatgtgt ttgacaaaca
     2461 gatgaatctc aagcgcaccc gcaacatcac cctgaaaggc atcaactatg ataccaatga
     2521 gctgatcaaa atgggagatc gcatctttca gttttatacg gaaagaagtg gccgtaccag
     2581 ttatctgaaa tttcaggaat acaatctggc tttggaaaac aaaggtgatc ctgtgttggt
     2641 tgacagcacc gtatccagaa ttggagaagc ctggtctgaa tacaaggtgc agcgttctga
     2701 accggatgaa tacatcttat gctataagtt cagtctgaac aacggtagga tggaacagac
     2761 ctatctccag atcctgaacc gggaaggcaa aatggtatct caaagtcagt tctacctgcc
     2821 tgccggcaat gacacgtatg aattgcttac cacccagata ggccgcggcg gtcagccggc
     2881 tttcctcttc cgtaacaatt catacaattg caaaaaagac gaagatggta ttcattactg
     2941 gatagccttt cccggcaacg gaggcaaact gtccacctgc ccgataagca atgcgggcga
     3001 tcaatgtatc aaagaactga atatcgggtt tgatgaggcc cgtaacatac ttgtggcagc
     3061 cggctttacc ggtgaagaca accggtattc catgcagggt tataccgtca tccatattga
     3121 ccctttaacc gggttggtaa caagcgaaaa gaacgatcag ttcagcgatg agctgagcag
     3181 cagtatcacc agcatgcttg acaagtattc gcggtacatt ccggcatatc aggtaacacg
     3241 tgtcattccc cgcatggatg gaggggctct gctggtggct gaatattacg acaagaccgt
     3301 agagaattac gactacacca attacgatcc gtattatggc taccgcactt ctaccagaca
     3361 ggttgagttt tacgaataca gtgatatcct gttgctaagc accggacctg aaggcaaact
     3421 ggactggagc aacatcatca gaaaaaaaca attgtctaaa gaagataaag gtgtcaattc
     3481 ttccttcgga ctgatgaaca gttaccgcaa cctgtatttt attttcaatg aagacatctc
     3541 acaaaacagc aatgtgctga tgtatgctct ttcagccgat ggcacacttg atcgtcagag
     3601 tctgttcaat ccatccaaac aggaagtgga actgcggccg gtagctgccc ggcagatcga
     3661 ctttgacgag gtcatcatcc ccagcatata taaacgtacc ttagccttcg tcaaggtcaa
     3721 actctgaagc gttgatacgc ctgttccgtt cagtcactcc gttttccctc ctggccggtc
     3781 tgctgtacct ggcggccatg tctttgcacc tgttcctgca tcctgttgta ccggatctgc
     3841 gtcctttgca ggaaccttat tctgactggt ttcctgttgc gcaatggagt gggcatgatc
     3901 tgtacatgat cgtactgctt gtcttgctta tgaccgctgc tttcatggtc agtgttactg
     3961 cagcaggcgc ctctttcacg catgggttat ctccgttgcc ggcaattgtg tacctgacac
     4021 tttgcttcct ggtgcctgat gcattcatcc atcctgtttc ggtcataact gttac
//
LOCUS       DAOMAX010000237         4007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4163_length_4007_cov_4.324646, whole genome shotgun sequence.
ACCESSION   DAOMAX010000237 DAOMAX010000000
VERSION     DAOMAX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_4007_cov_4.324646"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PK742_10745"
     CDS             complement(<1..851)
                     /locus_tag="PK742_10745"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HQU77110.1"
                     /translation="MRIELRSDTFTLPSKGMREAMAVADVGDDVFGEDPSVNSLEQLA
                     ASMLGTEAALFCPTATMCNQIAMKVHTRPMDEIICDRLAHVYNYEVGGFAFLSACSIR
                     TIETERGLLDPDSIIANINPDDVHKPVTRLVCLENTCNKGGGSIYPVQLMEQISQTCS
                     ANGLYLHLDGSRLFNAWVETGNELSDYGNWFDTITICLSKGLGAPAGALLCGTASTIR
                     EARRVRKVLGGGMRQAGILAAAGIYALRNNVERLKVDHEHAKQIGAILENQSWVDTCL
                     PVESNIV"
     gene            complement(848..1480)
                     /locus_tag="PK742_10750"
     CDS             complement(848..1480)
                     /locus_tag="PK742_10750"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQU77111.1"
                     /translation="MSISLTIIIIALTVITSMMAFNNPVMKGSALFYPHAISARGEWY
                     RFLTSGFIHADWMHLLVNMYVLYIFGDILEHYLLPLYFHQKTRLVYLAIYLGGMIVSD
                     IPSYFKHRHDSLYRALGASGAVSSIVFAGILINPWQGGIGLLFLPGITLPPVVFGGLY
                     LLYSAIMARRQSDNVNHDAHFYGALFGLILPGILQPEIFTSFFYQIMNRL"
     gene            complement(1480..1950)
                     /locus_tag="PK742_10755"
     CDS             complement(1480..1950)
                     /locus_tag="PK742_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQU77112.1"
                     /translation="MKISLLAMQKTTGKQLPVLIADYSGRASRYFPFTYKELPVKFPA
                     SVPSQVICSKEGEILLGQLDTSDQLWLLDERGDRLTSASFADFLVSRQQENVKHLVFA
                     IGGAYGFSPEVRNRSKGSIRLSDMTFTHQMVRLFFVEQLYRAGTIIRNEPYHHS"
     gene            complement(1972..2853)
                     /locus_tag="PK742_10760"
     CDS             complement(1972..2853)
                     /locus_tag="PK742_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQU77113.1"
                     /translation="MVKEASRHTALKTALWSVGLNTVFAVVKAFAGISGHSFALVADA
                     IESSTDILASLLVYAGLRYATRPADSNHPYGHGKAEPLITFLVVGFLVASALFIAHQS
                     WTNIQQPHEMPKAFTLPVLLVIIVVKEAAYRMVHRRSRQTGSSSLLADAWHHRSDAIT
                     SLLAFTGIALALLLGPGYEAADDWAALAASGFIMFNAYLIFRPALGEIMDEHQYDELL
                     NNIRKTAAQVTGVKGTEKCYIRKTGMSFHVDLHMQVDAQITVEEGHAIAHKLKDTLMR
                     QFPDIADILIHVEPFPQ"
     gene            2901..3236
                     /locus_tag="PK742_10765"
     CDS             2901..3236
                     /locus_tag="PK742_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77114.1"
                     /translation="MNRWILLLLCMFSLMACKRTRDEKVLASVMCDCAQPVVQWEAHL
                     KSDPSTLNQGAVIERDLRACLEDQKVRFAPYVEDTAFVYSVADRINEECPEVNTVVNA
                     MLLILAGKR"
     gene            complement(3239..3580)
                     /locus_tag="PK742_10770"
     CDS             complement(3239..3580)
                     /locus_tag="PK742_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone"
                     /protein_id="HQU77115.1"
                     /translation="MKYIVSLATIILSASLWSACSEAHAEKTVSFKVYGNCGMCEKTI
                     EGSLDQEGIYSASWDKDTKMVEIAFDSSRYRMEDLHHLIAVSGYDTDLEKAPDAAYES
                     LHECCQYERPL"
     gene            complement(3590..>4007)
                     /gene="lepB"
                     /locus_tag="PK742_10775"
     CDS             complement(3590..>4007)
                     /gene="lepB"
                     /locus_tag="PK742_10775"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQU77116.1"
                     /translation="DYIPLRYYPHDPELQLNSIDEFGPIWVPEKGVTVEINEKNYPFY
                     QRIIEFYEHNKVTHDSQGYPYIDGKKLTSYTFKQNYYWMMGDNRHRSLDSRFWGFVPE
                     DHIVGKASMVWWSWATEGSIFSRFGTIRWDRFMKIL"
BASE COUNT         1036 a          976 c          956 g         1039 t
ORIGIN      
        1 atcacaatat tggattctac cggaagacag gtatctaccc agctttggtt ttccagaata
       61 gccccgattt gttttgcgtg ttcatgatcc actttcaggc gctccacatt gtttctcagg
      121 gcatagatcc ccgccgctgc caggatgcct gcctgacgca tgccaccacc cagtaccttt
      181 cgcactctcc ttgcttccct gatggtactg gctgtgccgc ataacaaagc gccggctggt
      241 gcccccaggc cttttgacag gcagatggta atggtatcaa accaattgcc gtaatcgctt
      301 aattcattac cggtttccac ccacgcattg aaaagccttg agccgtccag atgaagatag
      361 aggccatttg cagaacaggt ttggctgatc tgctccatca gctgaacagg gtatatgcta
      421 ccaccacctt tgttgcaggt gttttccagg caaaccaggc gagttaccgg tttatgtaca
      481 tcgtccggat taatgtttgc aatgatggaa tccggatcca gtaatcctct ttctgtttct
      541 atggttctga tactgcaggc tgatagaaat gcaaaaccac ccacttcata attatagaca
      601 tgtgccagcc ggtcgcagat gatctcatcc attggtctgg tatgcacttt cattgcgatc
      661 tggttgcaca tggtagcagt ggggcaaaaa agggcagctt ctgtacccag catagatgct
      721 gccagttgtt ccagactgtt cacactcgga tcttctccaa atacatcatc accaacatcg
      781 gcaactgcca tggcctcccg cattcctttt gagggaagtg tgaacgtatc actgcgcaac
      841 tctattctca tagtctgttc atgatctggt aaaagaaact ggtgaatatt tcaggttgta
      901 aaataccggg taggatcagg ccgaaaaggg caccgtaaaa atgggcatca tgattcacgt
      961 tatcggactg tctgcgagcc ataatagctg agtacaagag atacagacca ccaaaaacta
     1021 ccggaggtaa tgtgattccc ggtaagaaca gcaggcctat tcctccctgc cacggattga
     1081 tcagaatacc tgcaaaaaca atagaggata ccgctcctga ggcaccaagt gcgcggtaca
     1141 gactgtcgtg acggtgtttg aaataggagg ggatgtctga gacgatcatc cctcccagat
     1201 agatggccag atagaccagc cgggtcttct ggtgaaagta taagggtaac agataatgtt
     1261 ccaggatatc gccgaaaatg tacaacacgt acatattgac cagcaggtgc atccagtcag
     1321 catgaatgaa accactggtc aggaacctgt accattctcc acgtgcagag atggcatgcg
     1381 gatagaataa tgccgatccc ttcatgaccg ggttgttgaa agccatcatc gaagtgatga
     1441 ccgttagggc aatgatgatg atggtcagac ttatggacat cagctgtggt gatatggttc
     1501 gtttctgata atggtacctg cccgatacaa ttgttccaca aagaacagtc ttaccatctg
     1561 gtgcgtgaag gtcatgtcac ttaacctgat actccctttg ctccggttgc gcacttccgg
     1621 agagaatccg taggcgcctc caatggcaaa aaccaggtgt ttgacattct cctgctgtct
     1681 ggatacaaga aaatctgcaa aagatgctga agtcaaacgg tcgccgcgtt cgtccagcag
     1741 ccacagctga tctgatgtgt cgagttgccc aagcaggatc tcaccttctt tggaacagat
     1801 cacctgtgat ggaacgcttg ccggaaattt gacaggtagc tccttgtagg taaacgggaa
     1861 gtaccgcgat gccctgccgg aataatctgc aataagcacc ggcaactgtt tgcctgtggt
     1921 cttctgcatg gccagcagcg atatcttcat cctgtaaatg taaaaagtat tctactgggg
     1981 gaaaggttct acgtgtatca gaatatctgc aatatcagga aactgacgca tcagggtatc
     2041 tttcagtttg tgtgcaatgg catgaccttc ttccacagtg atctgtgcgt caacctgcat
     2101 atgcagatcc acatgaaaag acatgcctgt tttacggata taacattttt cagttccttt
     2161 tacaccggtt acctgtgcag cggtttttct gatattattc agcagctcat catattgatg
     2221 ttcatccatg atctcgccca aggccggacg aaatatcaga taggcattga acataataaa
     2281 cccggatgct gcaagtgcag cccagtcatc ggcagcttca tacccggggc caagcagcag
     2341 ggcaagcgca atacctgtga aggcaagaag tgaagtgatc gcatcgctgc gatgatgcca
     2401 ggcatcggca agcaatgatg aactgccggt ttgcctgctt cgcctgtgaa ccatccggta
     2461 ggcggcttcc ttcaccacta tgatcaccag caggacaggt agtgtgaaag ctttgggcat
     2521 ctcgtggggt tgctgaatat ttgtccagct ctgatgtgca atgaataagg ccgaagctac
     2581 cagaaacccg accactaaaa aggtgatgag cggttcagcc ttaccgtgtc cgtaggggtg
     2641 attgctgtct gccggtcttg tggcatacct gagtcctgca tagaccagca gtgaggccag
     2701 aatgtcggtt gatgattcaa tggcatcagc caccagcgca aaagagtgtc cggaaatacc
     2761 ggcaaacgcc ttcaccacgg caaaaacagt gttcaggcca acactccata aggcggtctt
     2821 aagtgcggta tgccggctgg cttcttttac catcaaataa aggtatcgct tacaccggat
     2881 tcgggcagta tctttgttct atgaaccgtt ggatacttct cctgctctgc atgttcagtt
     2941 tgatggcatg caagcgaacc cgggatgaaa aagtgctggc ttctgttatg tgcgactgtg
     3001 cacagcctgt tgttcagtgg gaggcacacc tgaaatcaga tccgtccacg ctgaatcagg
     3061 gtgcggtcat tgaaagagat ctcagggcat gtctggaaga tcaaaaagta aggtttgcac
     3121 cctatgtcga agatactgcc tttgtttaca gtgtggctga ccgtatcaat gaagaatgtc
     3181 ctgaggtcaa caccgtggtc aacgccatgt tgctgattct ggcaggcaaa aggtgatctt
     3241 acaacggcct ttcgtactgg cagcactcat gcaaagattc gtaggcagca tccggagctt
     3301 tttccaggtc ggtgtcatat ccggataccg ctataagatg atggagatct tccatccggt
     3361 atctggagct gtcaaatgct atctctacca ttttggtgtc tttgtcccag ctggcagaat
     3421 agatgccttc ctggtccagt gaaccttcaa tggtcttctc acacatccca caatttccgt
     3481 aaaccttaaa gctgacggtc ttttctgcat gtgcttcact gcacgcactc cataaagagg
     3541 cactcaaaat gatcgtggca agagaaacga tatacttcat gtagagaaat taaagaattt
     3601 tcatgaaacg atcccagcgt atggtgccaa atctgctgaa gatacttcct tcagtagccc
     3661 agctccacca taccatgctg gctttaccta caatgtggtc ttccggaaca aacccccaga
     3721 agcgactgtc cagagaacga tgccggttgt cgcccatcat ccagtaataa ttctgtttga
     3781 atgtatagga ggtcagtttc ttgccgtcta tataaggata cccctggctg tcatgcgtaa
     3841 ccttattgtg ttcataaaac tcaatgatgc gctgataaaa cggatagttc ttttcattga
     3901 tctcaacggt gactcctttc tcaggtaccc agatcggtcc gaattcatct atagaattta
     3961 actgtagttc agggtcgtga gggtaatacc gcaatggtat ataatcc
//
LOCUS       DAOMAX010000238         3992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4193_length_3992_cov_3.452629, whole genome shotgun sequence.
ACCESSION   DAOMAX010000238 DAOMAX010000000
VERSION     DAOMAX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4193_length_3992_cov_3.452629"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PK742_10780"
     CDS             complement(<1..1563)
                     /locus_tag="PK742_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77117.1"
                     /translation="MKKYFTILMAASLSVPVIAQSAQTVDGDLHLLPRDTRSTPKSGV
                     YQDRSLDGTRESALTYYWINYSAAVDLTYNGLTLENISAVPLWPDSTVKVVGDDAGTP
                     FEYYWWVHSFSNVLDPTSTFIQDYVDYEYYPDAPPPVPTKEWFNDTHAYDVDSVGFYI
                     YYDRYVTDVTDTLYVYITTPGSSAHVFSTLGDPPYQAVYMRYNSTQNHPNGTLQEYMI
                     TLGNEDSASFAPKYISIPLDLEIPKDATGNDNKIGIAFSYHPGQSYSFGDTLLDFNDP
                     PLNTNPLNTLWLLVNEENFGALPTSMEDGAYNQSGNATSDVRYGISTTGWNGFYINTM
                     AFLSPEYAWEHPYVDWYLAPKGAAFVPTPTFPCQDLTLEFYDFSNFFEAGTATYYWAF
                     GDGGISFDANPVHTFPSPGTYEVCQVVTASSVSYEACKNVTVDFCTGVESFENLSNVS
                     LYPNPANDLLQWNAAFSGNEEITVEVLNSLGQLMYSESFSTADQFSGQLDVSNWSAGM
                     YLFRINNGQQNST"
     gene            complement(1638..2207)
                     /locus_tag="PK742_10785"
     CDS             complement(1638..2207)
                     /locus_tag="PK742_10785"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQU77118.1"
                     /translation="MFIEPLISEGKRGWIEVIAGCMFSGKTEELIRRLKRARIARQRV
                     EIFKPAVDTRYDEEHIVSHDENLIRSTPVSHSSNILLLGTGCHVIGIDEVQFFDQGVV
                     DIAQQLASSGTRVIVAGLDMDYSGKPFGPMPALLAVAEYVTKVHAICVQCGNIATHSY
                     RLSGSTEQVLLGEKDQYEPRCRSCFVKKL"
     gene            2262..2924
                     /locus_tag="PK742_10790"
     CDS             2262..2924
                     /locus_tag="PK742_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77119.1"
                     /translation="MSSFQEPVRLLQSVIELLEVRTEGTLSSLERDVVRQRLIHVYEM
                     LWDQQQPDTLNIPVEQETKEEAPVVQTVSLPEDDQVPDPEPQKEMKIPEPEPEVISVK
                     KEETPPQKEEKPPVSDDKKTNLNERFAGDKTTLADKILKPQGLLKNLIDVNEKFFFTR
                     ELFDGDRKAFEQAIEQLDACTGLEQAIGIINTDLATRYHWDTGSKAVDKFMKEILLRF
                     SR"
     gene            complement(2907..>3992)
                     /locus_tag="PK742_10795"
     CDS             complement(2907..>3992)
                     /locus_tag="PK742_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQU77120.1"
                     /translation="FHCASVGEFEQGRPLIELLKSRYQQIQIVLTFFSPSGYMARKNF
                     ALADLVLYLPADNEQNARRFMEMVRPSMAIFVKYEFWGSYLEALQQRKIPAVLLSAIF
                     RPGQPFFQWWGSYHRFMLTCFSEILVQDDASALLLHNIGILSVVCPDLRFDRVLQIAS
                     QSPEVGLIKEMSAHRIIIAGSTWPEDEQYLAEAYNRSWKTKGFKLIIAPHEPTEQHIQ
                     RLMKRFKGDAVRFSEATLPLCPKVLIIDSVGMLSSIYRYGYACYIGGGFGAGIHNTLE
                     AAVYGKPLAFGPRIQKFREARILTELKVATVITRAEELMRWTDSLTADENNYRVISEK
                     SSGFVAEHKGGAEKALKLISKYLPAEP"
BASE COUNT         1083 a          924 c          973 g         1012 t
ORIGIN      
        1 agtgctgttc tgctgtccat tgttgatgcg gaacaggtac attccggcgc tccagttgct
       61 tacatcaagc tgtccgctga attgatcagc agtagagaag ctttcgctgt acatcagctg
      121 accaagactg ttgagcactt ctacagtgat ctcttcattg cctgagaagg ctgcgttcca
      181 ctgcaggagg tcattggccg ggtttggata gagtgatacg ttactcaggt tttcaaatga
      241 ttccacacct gtacagaaat ctaccgtaac gtttttacat gcttcgtaag atacagaaga
      301 agcggttaca acctggcata cctcataggt tccggggctt ggaaaggtgt gtacagggtt
      361 ggcatcaaaa ctgatcccgc catctccaaa tgcccagtaa taggtagcag ttccggcttc
      421 aaagaaatta ctgaagtcat agaattccag cgtcagatcc tggcagggga aggtaggtgt
      481 aggaacaaag gcagctcctt taggagccag gtaccagtca acataaggat gctcccaggc
      541 gtattccgga gaaaggaagg ccatggtatt gatgtagaaa ccattccatc cggtcgtgct
      601 gataccatat cttacatcag aagtggcatt accactctgg ttgtaagcac cgtcttccat
      661 agaagtaggt aaggccccga aattctcttc attgaccagt aaccagaggg tattgagcgg
      721 gttggtattc agcggaggat cattgaaatc aagcagggtg tcgccaaaag aatagctctg
      781 gcccgggtga taagagaaag caatacctat cttgttatca ttaccggtag catctttcgg
      841 tatctccaga tccagcggaa tggagatata ctttggagca aatgaagcag aatcttcatt
      901 tcccagagtg atcatgtact cctgaagagt accgttcgga tggttctggg tgctgttata
      961 gcgcatgtaa acagcctgat agggcgggtc gcccagggtg ctgaaaacat gagctgaaga
     1021 gcccggagtt gtaatatata catagagcgt gtctgtcacg tctgttacat acctgtcata
     1081 ataaatatag aagccaactg aatctacatc atatgcatgg gtgtcattga accattcttt
     1141 tgtaggtaca ggaggaggag catccggata gtattcataa tccacataat cctgaatgaa
     1201 agtagaagta ggatccagta cattactgaa tgaatgaacc caccagtagt attcaaaagg
     1261 ggttccggca tcatcgccta ccactttgac ggtggagtct ggccacagcg gaactgccga
     1321 gatattctcc agggtgagac cattgtaggt caggtcaact gctgcactgt agttgatcca
     1381 gtaataggtt aaagccgatt ctctggttcc atccagactg cggtcctggt aaacaccaga
     1441 ctttggggta gagcgtgtgt ctcttggcag caggtgcagg tcgccgtcca cagtctgggc
     1501 gctttgcgca ataaccggga cagacaagga agctgccatc aggatagtaa agtatttttt
     1561 catcataatg actatttgat ctaacaaaga taatctaaaa caggggcaac ccatgtcacc
     1621 ttgtggtaag caaatgctta aagcttctta acaaagcaag accggcagcg gggctcatac
     1681 tgatcctttt ctcccagaag aacctgctcg gtgctgcctg ataaacgata gctgtgggtg
     1741 gcgatattgc cacattgtac gcatatggca tgtaccttgg taacatattc tgcaacagcc
     1801 agcaaggcgg gcataggccc gaagggcttg cctgaataat ccatgtccag ccctgccact
     1861 atgacccggg tgccggatga tgccagttgc tgggcaatgt ccacgactcc ctgatcgaag
     1921 aactgcactt catcgatacc gatcacatga cagcctgttc ccagcaacag gatattggat
     1981 gaatgtgaaa caggagtact tcttatgagg ttttcgtcgt gagaaacgat gtgctcttca
     2041 tcatagcggg tatctaccgc aggtttgaat atctccacac gttgccgggc tatccgggca
     2101 cgtttcagcc ttctgatcag ctcttctgtt tttccggaga acatacaacc ggctattacc
     2161 tcgatccagc cccgctttcc ttcactgatt aatggttcga tgaacattcg cagcgaaagg
     2221 tataaatttg ggcgaatatt gaataaactg aactcctctc catgtcatcc ttccaagaac
     2281 ctgtcaggct gctccaatct gtgatcgaat tgctggaagt gcgaacagag ggaacccttt
     2341 catcgctgga aagagatgtg gtcaggcaaa ggcttatcca tgtctatgaa atgctctggg
     2401 atcagcagca acctgataca ctgaatattc cggttgaaca ggaaacaaaa gaggaggcac
     2461 ctgttgttca aacagtgtca ttgcctgaag atgatcaggt gccggatcct gaaccacaga
     2521 aggagatgaa aataccggaa cctgaacctg aagtgatctc tgtgaagaaa gaggaaactc
     2581 caccacaaaa ggaagaaaaa cctcccgttt ctgacgataa gaaaacgaat ctgaatgaac
     2641 gcttcgccgg cgataagacc accctggctg ataagatact caagcctcag gggcttttga
     2701 agaacctgat tgatgtgaat gaaaaattct tttttaccag ggagcttttt gacggcgaca
     2761 gaaaggcctt tgaacaggct atcgagcagc ttgatgcctg caccgggctg gaacaagcta
     2821 ttggtatcat caatactgat ctggcaaccc ggtaccactg ggatactggc tccaaagcag
     2881 tagataagtt catgaaagag atacttctaa ggttcagcag gtaaatactt gctgatcaat
     2941 ttcagggcct tctctgcacc gcctttgtgt tccgctacaa aaccggatga cttttctgag
     3001 atcacacggt agttgttttc atcagccgtg aggctatcgg tccaccgcat aagctcttct
     3061 gctcttgtta ttacggtagc caccttcagt tctgtgagta tgcgggcttc ccggaatttc
     3121 tggatcctgg gtccaaatgc aagcggtttt ccatagactg cagcctccag ggtattgtga
     3181 attcccgctc cgaaacctcc tccaatataa catgcataac catacctgta aatggatgac
     3241 aacatcccaa cagaatcaat gatcaatact tttgggcaga gtggcaaagt tgcttcgctg
     3301 aacctgaccg catcaccttt gaatcttttc ataagacgct gaatatgttg ctctgttggc
     3361 tcgtgtggtg caataatgag cttgaatcct ttggttttcc aggaccggtt ataggcttct
     3421 gccagatatt gttcgtcttc aggccacgtg cttcctgcta tgatgatgcg atgtgctgac
     3481 atttccttga tcagccctac ttcgggcgat tgggatgcga tctgcaatac cctgtcaaaa
     3541 cggaggtcag gacatacaac tgacagaata ccgatgttgt gcaacagcag tgcagaggca
     3601 tcatcctgaa caagtatctc actaaagcag gtcagcatga agcggtggta acttccccac
     3661 cattgaaaaa atggctgacc ggggcgaaat atggctgaaa gcagaacagc aggtatcttc
     3721 ctttgctgca atgcttcgag gtagcttccc cagaattcat atttgacaaa aatggccatc
     3781 gagggtctga ccatttccat aaaacgccgg gcattctgtt cattatcggc cggcaggtac
     3841 agtaccaggt cagccagggc aaaattcttt ctggccatat aacctgaggg ggagaagaag
     3901 gtcagaacga tctgaatttg ctggtacctc gatttcaaga gctctatgag gggccttcct
     3961 tgttcaaatt cgccaacaga agcgcaatga aa
//
LOCUS       DAOMAX010000239         3992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4194_length_3992_cov_3.430785, whole genome shotgun sequence.
ACCESSION   DAOMAX010000239 DAOMAX010000000
VERSION     DAOMAX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_3992_cov_3.430785"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="PK742_10800"
     CDS             <1..1289
                     /locus_tag="PK742_10800"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77121.1"
                     /translation="NIGSEVVIELGKGYYDDYLFDFNWTETATASSGDWEWGDPVGTE
                     YGIGYCNPEDDIAGDYGSFCYITGNGGGSAGSDDVDDGYVTLTSPSFDLSTFGDPWLT
                     FDFWFFNNGGSGTPNDTLSMILDNGLTTFEIVLDNGFTALGLWQNVSFQFSDYITPTA
                     NMHVSFKTGDNAASGHLVEAAIDRFFVFDSLTTAPVAAFDADLEDGCTPLHVHFYDLS
                     AGATSWSWSFPGGSPATSTLASPIITYNTPGIYDVTLIATNPLGSNTLTLTSYIEVFA
                     SPVLATSAGAGSASVIISGGTAPFDILWSDGQTTETATGLEVGTYTVTVTDANGCSVN
                     ATAEVTQPSGIDWLEDGLSVTVFPNPATEYVHVVSDASGSIFWSMTDLQGRTVLESNI
                     DPGETIIAVDGLSSGLYHVQLIDAGRLVGILSVVVE"
     gene            1297..2850
                     /locus_tag="PK742_10805"
     CDS             1297..2850
                     /locus_tag="PK742_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQU77122.1"
                     /translation="MRLRQLLLLSCLPLVSVAQENMTLQSWYSYDERAADVWGWESED
                     GHFYAIIGIFDGTSIVDITDPSSPIEVAFVGGPESIWRDIKTWNDHAYVVNETGGGML
                     IIDLSDLPGVPDTTSYTGDSLATAHNIFIDEHGIAYVIGSNRHNGGALFLDLNSDPMA
                     PEQIGYYDVNYCHDLYVRGDTMWTAELYVGQFGVVDVSDKASPVVLATQTTPAFFTHN
                     LWLSDDGDYLYTTDEVSGAPVAAYDVSDLSDIRLVDTWESSPGAGVIPHNTFYHDGYL
                     VTSYYRDGVTIVDAHRPDNLIETGYYDTSPFPSGNGYNGCWGVYPYFSNDLIAASDIE
                     EGLFILQPDYQRASYLEGVVSEEGTGMLLPGSLVEILGTEQTSITDFLGGYKTGTSAP
                     GLYDVRVSKSGCLTEIASGVELLQAEVTSLNFNLTCTASVAIEHPEDIILEVNPSVFQ
                     DHCTLTWNEISEALEIQIFDLCGRLVESMSMDTRNGRIDLGADLHPGIYLIDLQSDDL
                     RQQVRVIRL"
     gene            2906..>3992
                     /locus_tag="PK742_10810"
     CDS             2906..>3992
                     /locus_tag="PK742_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HQU77123.1"
                     /translation="MKKYLLFPALMAIAISGLSQTVVFSDDFESGAANWDIDGYWGVT
                     DEYAYGGGFSFTDSPWDPTYTAGEVQTATMVTSVDLSAALDAEVTFYALIDLEEGFDY
                     MYLDASDDDGAGWINIGVFNGEGMLTDWMPFTFGLGAFVGSPTVKLRFRFEPDFFVEY
                     DGMYIDDFTITSYTTDASPPLILHDVMPLYQGALYENELTATLLDASGISSTSLWYSA
                     DGGAYSSVSGVSLGGDDYGYTIPVMDPGTWITYYFTATDAADDLNTATSPEYEIITGQ
                     YIGYDDGVIDFVNDIGNASLSGYSQAAVRITLDSFATVVSLVIQNYTDNTRPNDSILI
                     HVWADDAGLPGADLIEPFKLFPEATLEL"
BASE COUNT          939 a          923 c         1014 g         1116 t
ORIGIN      
        1 tcaatatcgg gtcggaagtg gtcatcgaac tcggtaaagg atattatgat gactatctgt
       61 ttgatttcaa ctggactgaa actgctactg catccagtgg cgactgggag tggggtgatc
      121 ctgtaggaac cgaatatggt atcggatatt gtaatcctga agatgacatt gccggagact
      181 atggcagttt ttgttatata accggcaatg gcggtggttc agccggttct gatgatgtag
      241 atgacggtta tgtaacactg acgtctccat cttttgatct gagtaccttc ggtgatccgt
      301 ggcttacctt cgatttctgg ttctttaaca acggaggttc aggcactccc aatgacacct
      361 tgtctatgat cctggataat ggtctgacca cttttgaaat cgtgttggac aacggcttta
      421 ctgcactggg tctgtggcaa aatgtatcgt ttcagttcag tgattacatc actccgactg
      481 ccaacatgca tgtttcattc aagaccggtg ataacgctgc cagcggtcac ctggtggaag
      541 cagccattga tcgtttcttt gtgtttgatt ctctgaccac tgctccggta gcagcctttg
      601 atgccgatct ggaagatggc tgtacaccac tccatgtgca tttctatgac ctctctgcag
      661 gggcaacttc ctggtcatgg tctttcccgg gcggaagtcc ggccacttct acactggcaa
      721 gtccgatcat cacatacaac acacccggca tctatgacgt gacactgatt gcaaccaatc
      781 cgcttggaag caacacgctc accctgacca gctatattga agtatttgcc tctcctgttc
      841 tggcaacaag tgcaggagca ggttcagctt ctgtgatcat ttccggtgga accgctccgt
      901 tcgatattct gtggagcgat ggtcagacaa ctgagacagc taccgggctg gaagtaggca
      961 cctataccgt taccgttaca gatgccaatg gctgtagtgt gaatgctact gcagaagtga
     1021 cacagccttc cggtattgac tggctggaag acggattgtc tgttaccgtg tttcctaatc
     1081 ctgcaactga atatgtgcat gtggtgtctg atgcttccgg ttctattttc tggagcatga
     1141 ccgacctgca gggtcgtacc gtactggaat caaacattga tcctggtgag acgatcatag
     1201 cagtggatgg actgtcttcc ggtctgtacc atgtgcagct gatcgatgcc ggcaggctcg
     1261 tggggatact tagtgtggta gtggaatgac gatggtatgc gattgagaca actcctgctg
     1321 ctttcctgtt tacccctggt ctcagtggca caggagaaca tgacgctgca atcatggtat
     1381 agttatgatg aaagggcggc tgatgtgtgg ggctgggaga gcgaagacgg acatttctat
     1441 gctatcatcg gcattttcga tggtacatct attgttgaca tcaccgatcc ttcttcacca
     1501 atagaggtgg cctttgtagg cggaccggaa agtatctgga gagatatcaa gacctggaat
     1561 gaccacgctt atgtggtcaa tgaaacagga ggaggcatgc tcatcataga cctgtctgac
     1621 ctgccgggcg ttccggatac caccagctat accggcgatt ctctggctac agcccataat
     1681 atctttattg atgagcatgg tatcgcctat gtgataggca gcaaccggca taatggcggc
     1741 gctctttttc ttgacctgaa cagcgaccct atggctcctg aacagatcgg ctactatgat
     1801 gtcaactact gccatgatct ctatgttcgc ggagatacca tgtggaccgc agaactctat
     1861 gttggacaat ttggcgtggt ggatgtttct gataaagctt caccggttgt gctggccacc
     1921 cagaccactc ctgcattctt cactcataac ctatggctga gtgacgatgg cgattacctc
     1981 tatacaactg atgaagtttc cggtgctccg gtggcagcct atgatgtgag tgatctcagt
     2041 gatattcgcc tggtcgatac ctgggagtca tcgcccggag cgggagtgat accgcacaac
     2101 actttttatc atgacgggta tcttgtaact tcttattacc gcgacggagt gaccatcgta
     2161 gatgcacaca gaccggataa cctgatcgaa acaggctatt atgatacttc cccctttccc
     2221 tccggtaacg gatacaacgg ttgctggggt gtctatcctt atttcagcaa tgatctgatc
     2281 gcagcatctg atattgaaga aggcctgttc atcctccagc cggattacca gcgggcttcc
     2341 tatctggaag gggtagtatc tgaagaggga acaggcatgc tgcttcctgg ttctctggtt
     2401 gagatcctgg gaacggaaca gaccagtata acagactttc tgggaggcta taagaccgga
     2461 acttcagctc cgggactata cgatgtgagg gtgtcaaaat caggatgtct gactgaaatt
     2521 gcatccggtg tggaattatt acaggcggaa gtgacctctc tgaatttcaa ccttacctgc
     2581 actgccagtg tagccattga acaccccgaa gacatcatac tggaagtgaa tccctctgta
     2641 tttcaggatc actgtaccct gacctggaat gagatttctg aagcgctgga aatacagatc
     2701 ttcgacctgt gcggccgcct tgttgaaagc atgtctatgg ataccaggaa cggccgaatt
     2761 gatctcgggg ctgaccttca tcccggtatt tatctgatcg acctgcagtc agatgacctg
     2821 cgtcagcagg taagggtgat cagattgtag gtttgagtat gatgtaaatt gcgtatttta
     2881 ggtgcacaac aaaaatggtt gcaccatgaa aaaatatcta cttttccctg ccttaatggc
     2941 tatagctatc agcggattat cccaaactgt tgttttttct gatgattttg aatcgggtgc
     3001 tgctaactgg gacattgatg gttattgggg tgttactgat gaatatgcct atggtggtgg
     3061 tttctctttt accgattctc catgggatcc tacctatact gcgggtgaag tacagactgc
     3121 aacaatggtc acttctgttg acctctcggc cgctcttgat gcagaagtga ccttctatgc
     3181 actgatagat ctggaagaag gatttgatta catgtacctg gatgcctctg atgatgatgg
     3241 tgccgggtgg atcaatatcg gggtgttcaa cggcgagggc atgcttacag actggatgcc
     3301 ttttaccttc ggtctcggag catttgttgg cagccctaca gtaaagcttc gctttcgttt
     3361 tgaacctgat ttttttgtgg agtatgatgg catgtacata gacgatttta cgatcacttc
     3421 ctatactacc gatgcttcac ctccgcttat tctgcacgat gtgatgccgc tgtatcaggg
     3481 tgccttgtat gagaacgaac taacggcaac actgctggat gccagcggaa tcagctctac
     3541 atcattatgg tacagtgcag acggtggcgc ctattcatct gtttcagggg tcagcctggg
     3601 tggcgatgac tacggctata ccatacctgt catggatccc ggtacatgga tcacttatta
     3661 ctttacagca actgatgctg ctgatgatct gaatactgcc acatcgccgg aatatgagat
     3721 cattacaggg cagtatatcg gatacgatga tggcgtaatt gactttgtga atgatattgg
     3781 aaatgcgtca ctgtcaggat attcacaggc cgcagtacgc atcacactgg atagttttgc
     3841 tacggtagtg tcattggtca ttcagaatta tacagacaat accagaccca atgacagcat
     3901 cctgatccat gtctgggcag atgacgcagg cctgccggga gctgacctca tcgaaccctt
     3961 taaactcttt cctgaagcca cactagaatt gc
//
LOCUS       DAOMAX010000240         3978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4226_length_3978_cov_3.229926, whole genome shotgun sequence.
ACCESSION   DAOMAX010000240 DAOMAX010000000
VERSION     DAOMAX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_3978_cov_3.229926"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK742_10815"
     CDS             <1..640
                     /locus_tag="PK742_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77124.1"
                     /translation="KADPDNKSKRKVLITGLSLLDANNTSQKMNFSERGISKIQALKK
                     VIEDKQDIADFETTWQPIFKTFKTHESIEEMILLVSKDTDFEDRQLADNADWNICRFD
                     NIMQTWFPDIKYYKLTLEDPYMVEPIVEHVRAELSVRCRKRKDVELLFGITGGTAPTS
                     SALSMLAVKGSRGITYTSQDASLREKKPVLDEEINVFTVKDLWQELIDKAEK"
     gene            645..938
                     /gene="crn3"
                     /locus_tag="PK742_10820"
     CDS             645..938
                     /gene="crn3"
                     /locus_tag="PK742_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Crn3/Csx3"
                     /protein_id="HQU77125.1"
                     /translation="MHFELKEDEKWVVIHFEGEGIILPEELRTISPPDLVKLKLSHKG
                     VVLSGRGPVWLYSFLTHFYHPAAFIATFDTHLNKAVVTSSHVSGFSEGDILEL"
     gene            950..1729
                     /locus_tag="PK742_10825"
     CDS             950..1729
                     /locus_tag="PK742_10825"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HQU77126.1"
                     /translation="MASPTPILSIPFDIKVQHWELQFIRAAVANDVLSKKPLFEAAGL
                     ATDYFHNHDEADDRDGRTVNRYPLIQYTEKNDYLCLLAVGPAVDALLFYLKHMPGRLQ
                     FRNRIVAMLPVLSYARFQHYTAEILEKPAKYQLRQWIGLQEHRHAIWKQTPRMEDRVQ
                     LLNEALLGSVKRMLTEGLNQSLDPAAECYLHDIFQADHTTYKKRQWRCFTLEFYSNLL
                     LPAELGLGKGCSIGYGKLLAPASLIKAEPAFPDANARTGVL"
     gene            1760..2806
                     /locus_tag="PK742_10830"
     CDS             1760..2806
                     /locus_tag="PK742_10830"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HQU77127.1"
                     /translation="MGSSSLRFFRSNAKRFASVRSKEQLAQELDTSKYTLELLAVSPP
                     YRSFKIPKKDGTWRTIEEPEEDLKEVLKLLAFHLQTVYFFIKPPCAYGYILSHRSDPD
                     KRNIVTNAHRHIQAHTLLNVDMRDFFHQCDEAELVSVLRPHFPEMEQDCLELIANLTT
                     YHNRLPMGSPSSPVLSNYAAFRLDHAMLAWSQERNIVFTRYVDDMTFSSRTVLPVEYV
                     KQLEEACAKFGFPLNPAKTKYYGPEDEKYVTGLMLTKEEVVLPGKYFSYLQNDLDRFA
                     ALYPVFSCLSDERSSAWVRHFEQGLTGRINHIGAVYGRSSATFRHYQKKLQAAKRPVA
                     EEIYMNWLDLPYET"
     gene            2808..3803
                     /gene="cas1"
                     /locus_tag="PK742_10835"
     CDS             2808..3803
                     /gene="cas1"
                     /locus_tag="PK742_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HQU77128.1"
                     /translation="MQLYIHTRGTMVRVRNRSFHISNGEEVRMVSAHRVTSMAIGNSC
                     TLSSSALLLALKHHIPVYLLDGIGRISGQVSGTGFSNHTSIRRQQVLQKDSDWSKRYI
                     IDLIVTKLQRQQAHINILLDGISHANSTKAVYFVRSVGEVVAALNNNTQEGELTDLSV
                     LSGWEGQLARNYWGLLSSGLPAGFQFEGRSRMPATDRFNAALNYLYAILGSYVENAII
                     QAGLDPHFGFMHADGYGSKGLTYDLMEPFRPWADKCLTSLINNDLLKETHFQVNEDEV
                     RINADARRIIITGWNDFLEETELLNDRQVAKKNHFFLLATGLSATLLAQINESNE"
     gene            3796..>3978
                     /gene="cas2"
                     /locus_tag="PK742_10840"
     CDS             3796..>3978
                     /gene="cas2"
                     /locus_tag="PK742_10840"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQU77129.1"
                     /translation="MSEANDHLFLVCYDIQNDRKRTKIADLLMEYGYERLQYSVFVGL
                     QEPSANERLWGRLSELV"
BASE COUNT         1149 a          802 c          965 g         1062 t
ORIGIN      
        1 aaaggcggat cctgataaca aaagcaaaag aaaggtgctg attactggtt tgagtttgtt
       61 ggatgccaat aatacatccc aaaaaatgaa ttttagcgaa agggggatta gtaagattca
      121 ggcattgaaa aaagtaatag aagataagca agatatagct gactttgaaa ctacctggca
      181 acctattttc aaaacattta aaacgcatga atcaattgaa gagatgatcc tactggtgtc
      241 taaggatacc gattttgaag atcgtcagtt ggcagataat gctgattgga acatttgtcg
      301 ttttgataac atcatgcaaa cctggtttcc cgatataaag tattataaac taacattaga
      361 agacccctat atggtggaac caattgtaga gcatgtcaga gccgagttaa gcgttcgatg
      421 caggaaaaga aaggatgttg agctgctttt tggtattacg ggaggtactg cacccacctc
      481 cagcgcactc tccatgcttg cagtaaaagg atccagaggt atcacctata caagtcagga
      541 tgcttcatta agggagaaga aaccggtact tgatgaagag atcaatgttt ttactgtaaa
      601 agacctctgg caggaactga tcgataaagc tgaaaaataa ttttatgcat tttgaactga
      661 aagaagatga aaaatgggtg gttatacatt ttgaagggga aggaatcata ttgcctgaag
      721 aacttagaac catttcgcca ccggatctgg tgaagttaaa gctcagtcat aaaggtgtag
      781 ttctgtcggg cagaggccct gtttggctgt atagtttcct gacgcacttt tatcatcctg
      841 cggctttcat agccaccttt gatacccatt tgaacaaagc tgtggttacc tcatcgcatg
      901 taagtggatt ttctgaagga gatattttag aattgtaatg ttgatgtaca tggccagccc
      961 cacccccata ttatccattc cctttgatat caaggtgcag cactgggagc tgcagtttat
     1021 ccgtgctgca gtggccaatg atgtactgag taagaagccg ttgtttgaag ctgcaggtct
     1081 ggccactgac tatttccata accacgatga ggctgatgac cgcgacggac gaaccgtaaa
     1141 ccgctacccg ctcatacaat atacagagaa gaatgattac ctctgtctgc tggccgttgg
     1201 gcctgcggta gatgccttgt tgttctacct gaagcacatg cccggccgcc tgcagtttcg
     1261 taaccgcatt gtggccatgc tgccggtact gtcttatgcc aggtttcagc attacacagc
     1321 agaaatactg gaaaaaccgg caaagtacca gttgagacaa tggataggat tacaggagca
     1381 cagacacgca atatggaaac agacacccag gatggaagac cgcgtgcaac tcctgaatga
     1441 agcactctta ggttctgtca agcgcatgct gactgaaggt ttgaaccagt ctttggatcc
     1501 tgcagctgaa tgttacctgc atgatatttt ccaggcggat cataccacgt ataaaaagcg
     1561 ccaatggcgt tgtttcaccc tggaatttta cagcaacctt ctcctgcccg cagagttggg
     1621 tctgggtaag ggctgcagca taggttatgg caaactgctg gcacctgcct ccctgatcaa
     1681 ggccgaaccg gccttcccgg atgccaacgc cagaaccggt gttctttgac atattgaatc
     1741 attatacccg catatgcaca tgggtagcag cagccttagg ttcttcagat caaatgcaaa
     1801 acgctttgca tcagtcagat ccaaagaaca gcttgcccag gaattggata cttccaaata
     1861 tacattggag ctgttggccg tttcacctcc ttaccggagt tttaagattc caaagaagga
     1921 tggcacctgg cgcaccattg aagagccgga agaagacctg aaggaggtgc tgaagttgct
     1981 ggcctttcat ttacaaacag tctatttctt tatcaagccg ccttgtgctt atggttatat
     2041 tctgagccat cggtctgatc cggataagcg aaatattgta accaacgcac atcgtcatat
     2101 acaggcacac acgctgctca atgtggatat gcgggatttt tttcaccagt gtgatgaagc
     2161 tgaattggta tctgtactca ggcctcactt tcctgaaatg gagcaggatt gtctggagtt
     2221 gattgcaaac ctgaccacat accataacag actgccaatg ggtagcccct caagcccggt
     2281 gcttagtaat tatgctgcct tcagactgga ccatgccatg ctggcctgga gtcaggagag
     2341 aaatattgtc tttaccaggt acgtggatga tatgacattt agcagcagga ctgtattacc
     2401 ggtagaatat gtgaaacaat tggaagaggc ttgcgctaag tttggttttc ccctgaatcc
     2461 tgccaaaaca aaatactatg gcccagagga tgaaaagtat gtgactggtc tgatgttgac
     2521 aaaagaagaa gtagtcttgc ctggaaagta tttttcttat ttgcagaatg acctggatcg
     2581 ttttgcagcc ttgtacccgg tgtttagttg tctgagtgat gagcggtcgt cggcctgggt
     2641 aaggcatttt gaacaaggac tcacaggaag gatcaatcat ataggtgctg tgtatggtag
     2701 aagctctgca acattcaggc attaccagaa aaaactgcaa gcagcaaagc ggcctgtagc
     2761 agaagaaatt tatatgaact ggctcgatct gccttacgag acttgatatg caattgtata
     2821 tacataccag aggaaccatg gtcagggtaa gaaaccgttc tttccatatc agtaatggag
     2881 aagaagtgcg catggtgagt gcacatcggg ttaccagtat ggctataggc aatagctgta
     2941 cgctttcttc atctgcattg ttattagcgc tgaagcacca tatccctgtt tatctgttag
     3001 atggtatagg aaggatttca ggtcaggttt cgggaacagg cttttctaat cacactagta
     3061 tcagaagaca acaggtttta cagaaagatt ctgactggtc taagcgatat attatagatc
     3121 tgattgttac aaagctccaa aggcagcagg cacatattaa tattctttta gatgggattt
     3181 cacatgcaaa cagtactaaa gccgtttatt ttgttcgctc agtaggtgag gtagtcgctg
     3241 cactcaataa caatacacag gaaggtgaat tgacagattt atctgtatta tccggatggg
     3301 aaggtcaact agccagaaat tactggggct tattgtcttc aggcttgcct gcggggtttc
     3361 agtttgaagg ccgcagcagg atgcctgcaa cagacaggtt caatgcagca ctgaactacc
     3421 tgtatgccat attgggcagt tatgtagaaa acgccatcat acaggcagga ctggatccgc
     3481 atttcgggtt tatgcatgca gatgggtatg gcagcaaagg actaacttac gatttgatgg
     3541 aacctttcag gccctgggct gacaagtgcc tgacatctct gataaataat gacctgttaa
     3601 aggaaaccca ttttcaggtt aatgaagacg aggtcagaat taatgcagat gccagacgta
     3661 tcattattac gggctggaat gactttttgg aagaaacaga gttgctgaat gacagacagg
     3721 tagctaaaaa gaaccatttt tttctgctgg ccaccggatt atctgcaaca ttgctggctc
     3781 agataaacga aagcaatgag tgaagcgaac gatcatctgt ttctggtatg ttatgatatt
     3841 cagaatgacc ggaagcgaac taagatagct gacctgttaa tggagtatgg atatgaaaga
     3901 ttgcaataca gtgtgtttgt tggattacag gagccttcgg cgaatgaaag gctatggggc
     3961 cggttatcag aactggta
//
LOCUS       DAOMAX010000241         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4227_length_3977_cov_4.044365, whole genome shotgun sequence.
ACCESSION   DAOMAX010000241 DAOMAX010000000
VERSION     DAOMAX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_3977_cov_4.044365"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1717)
                     /locus_tag="PK742_10845"
     CDS             complement(362..1717)
                     /locus_tag="PK742_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQU77130.1"
                     /translation="MDILTTYNPSTGEKLADYPLMQQEELDRRLQEAGEAYRYWSALE
                     VAQRSFAVGNLARELDRDLDDLARLMSEEMGKIRPEAEAELKKCIRLCEYYAAHAEAW
                     LADEDRSHGEVKAMVQYAPLGAVLAIMPWNFPVWQVLRCAVPALLAGNVVLLKHAPNV
                     TGCALQLDDLFVAAGFPEYTFQSLVIDVDQVESILARPLIAGVALTGSTRAGAAVAQL
                     AGKHIKRSVLELGGSDPFIVLADADIDKAVETAVQSRFGNAGQSCIAAKRWIVVEEVY
                     ESFRVKAIQKIRSLRQGDPVQDDIQVGPMARPDLADQFMEQYNRSVSEGAEVLVPCIR
                     NACHVSPGLLGDVTPEMTAFREELFGPCGVLIKAKDADDAIRLANDTIYGLGSTIFTA
                     DHALAMQLAQQIHSGNVFINTIMRSDPALPFGGVGMSGYGKELGREGILEFVNKKVVV
                     G"
     gene            complement(1780..2196)
                     /locus_tag="PK742_10850"
     CDS             complement(1780..2196)
                     /locus_tag="PK742_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77131.1"
                     /translation="MRLVSKLPATAVLSLLPFFVSAQVIGIWIQDVDDDPLTLILEED
                     GSATIILGDDIFGGLDYETNRGMATMKYRTESAGDLQRLTITMTLQSNGEVLLTYYGL
                     YRILGDGRMQLALYPEEEGFPDELTDDNSMWLTNYD"
     gene            complement(2273..2680)
                     /locus_tag="PK742_10855"
     CDS             complement(2273..2680)
                     /locus_tag="PK742_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77132.1"
                     /translation="MRTVLFTAALLLGLAVQAQSDKIVGRWTGDDMGEPITLVFDEEG
                     FCTILSGTDVVGGPNMDVGGFPGYMAYEVSFGDPYNFIDLNVYLKEDGSFLGGMPGIF
                     TLDGKEMHICLNFNGEVRPSAFVEDDSLWLTKE"
     gene            2778..3425
                     /locus_tag="PK742_10860"
     CDS             2778..3425
                     /locus_tag="PK742_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77133.1"
                     /translation="MKTFNIRYFIGELIIVTAGVLIAFQLSQVKESRQDHELTKTYLE
                     SLHADLLEDSTDIYTSIQQIAEFQTYLQGIIPHFFRELPGRDTVVQSMFKHTQFIHFI
                     AHDATYNTMESTGSLALLDDASLKIAITRHYDSYQSLYDENERIENFSRSYYATYMME
                     HVDQMELFMYGKPCCFDDTRFRNLVFSMTGILQIQEKTYREALERCRDLISKLEV"
     gene            complement(3422..>3977)
                     /locus_tag="PK742_10865"
     CDS             complement(3422..>3977)
                     /locus_tag="PK742_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77134.1"
                     /translation="DNPLFDKLNALNGDELIHIMEQAATGEDTEENGLIWSTGFDIAD
                     KKGWHEYFMEYFIRKGIIAETEDSEKDTIPPEELERQSLEDLAVSGFRITMEEPGMTT
                     LIRELQQASIPYLKLEQPGIPVVCVYEDDVPQIHVIMDKFGLISKREMKPPAYNAAIS
                     KAIIIAAALLLFGIAMLAFFIMQT"
BASE COUNT         1007 a         1065 c          875 g         1030 t
ORIGIN      
        1 gactgggcat ctggcccgag atcacgaaga tcagattgat cagagaataa gaaaacaatg
       61 tgaaaatggg agaatgtgaa aatgtgctga ggcgtcaaat gattttgcac aagagaaatt
      121 caaaattcat taatacattt tttcatccta aatgcagcga tcccacgatc acgcagcagt
      181 tacatcaata ttactaaaga gcatcgctgc cttccaactg gcatttcatt ttcacattag
      241 ctaattttac attttaacat tcgcccattc tcccattctc ccattctccc attttcacat
      301 tttcacattt tcacattccc ccattctccc attttcccat tttcacattt tcactctcca
      361 actatcctac caccactttc ttattcacga attccagtat cccttccctg cccagttcct
      421 taccatagcc agacataccc acgccaccga atggcaaagc aggatcactg cgcatgatgg
      481 tattgatgaa cacgttgccg gaatggatct gctgagccag ctgcattgcc aatgcgtgat
      541 cggccgtgaa gatggttgag cccaatccat agatggtatc gtttgcaagc cggatagcat
      601 catcagcatc tttggccttg atcagcaccc cgcagggacc gaagagttct tcgcgaaaag
      661 cggtcatttc gggtgtgaca tcaccgagta atcccggact gacatggcat gcattccgga
      721 tgcagggcac cagcacttct gcaccttctg acacagatct gttgtattgc tccatgaact
      781 gatcagcgag gtctggtctg gccatcggac ccacctgaat atcatcctgt acaggatcac
      841 cctgacggag gcttcggatc ttctgaatag cttttaccct gaacgattca tagacttcct
      901 ctaccacgat ccatcgcttg gcagcgatac aggactgtcc tgcattgcca aacctggact
      961 gcactgccgt ttccactgcc ttgtctatgt ctgcatcagc cagcacgatg aaaggatcgg
     1021 aaccacctaa ctcaagcaca cttcttttga tatgttttcc ggccagttgc gccaccgctg
     1081 cacctgcacg ggtggaacct gtaagcgcta caccggcaat cagcggccgg gcgaggatgc
     1141 tctccacctg atccacatct atgaccagcg actgaaaagt atattccggg aaacccgccg
     1201 ccacgaacag gtcatccagt tgcaaagcac atccggtcac gttcggagcg tgcttgagca
     1261 acaccacatt acctgcaagc agggcaggca cagcacagcg gagcacctgc cacaccggaa
     1321 aattccaggg catgatggcc agcactgcac ccagcggtgc gtattgtacc attgctttca
     1381 cttctccgtg agaacgatct tcatctgcca gccaggcttc tgcatgtgcg gcatagtatt
     1441 cacacaacct gatgcatttc tttagttctg cttccgcttc cggcctgatc tttcccattt
     1501 cttctgacat gagtctggcc agatcgtcca gatcacggtc cagttcacga gccagatttc
     1561 ccactgcaaa tgatcgctgt gccacttcca gcgccgacca gtaccggtat gcttctccag
     1621 cttcctgcag gcgcctgtcc agttcttcct gctgcatgag cggatagtct gccagtttct
     1681 ctccggtaga tggattgtag gttgtcagaa tgtccatgtc agatagctta caacgagcat
     1741 gcaagttacc aagaacctga cttgttcgtt attcaggaat taatcgtagt tggtaagcca
     1801 catggagtta tcatctgtca gctcatcggg gaacccttcc tcttcgggat agagtgcgag
     1861 ctgcatcctg ccatcaccca gaatccggta aaggccgtaa taggtcagca gcacttctcc
     1921 gtttgactgc agggtcatgg ttatggtcag ccgctgcaga tcgccggcag attctgtacg
     1981 atacttcatg gtagccatac cacggttcgt ttcataatcc agacctccaa aaatgtcatc
     2041 accaagtatg atggtagccg acccgtcttc ttccaggatc agggtaaggg gatcatcatc
     2101 cacatcctgg atccagatac caatgacctg ggcagacaca aaaaaaggca acaggctgag
     2161 aacagcagtt gccggcagtt tggaaaccag acgcatagaa ttgttttccg aaagatacta
     2221 tgcttcaaaa aaaaaccggg ccaggcccgg ttgtaatcag aacaagatga tgttactctt
     2281 tcgtaagcca gagtgaatcg tcttccacaa aggccgaagg tctaacttca ccgttaaagt
     2341 taagacatat atgcatttct tttccatcca gggtaaaaat tcccggcata cctcccagaa
     2401 atgaaccgtc ttctttcaga tacacattca ggtcaatgaa gttatacggg tctccgaagc
     2461 tcacctcata ggccatatag cccggaaagc ctcccacatc catattcggc ccgccaacta
     2521 catctgtacc cgacaggatg gtgcaaaaac cttcttcatc aaataccagg gtaatgggtt
     2581 cacccatgtc atcgccggtc catctgccaa cgatcttgtc gctttgcgcc tgcactgcca
     2641 ggcccagcag cagggctgcc gtaaaaagta ctgttctcat tgttgttgtt tgttatagga
     2701 gtattggggg cgtttaaatc catagtgaag gtaagggttt tcacattctt taaccttttc
     2761 attattttcg gagatacatg aaaaccttta acatcagata cttcattggt gagctgatca
     2821 tcgtaactgc cggtgtactc atagcctttc agctcagtca ggtcaaggaa tccaggcagg
     2881 accacgagtt gacaaaaacc tatctggaga gccttcatgc cgacctgctg gaagacagca
     2941 cagacatcta tacttcgata caacagatcg cagaatttca gacctatctg caggggatca
     3001 tcccccattt cttccgggaa ttgccgggac gtgatactgt ggttcagagc atgttcaagc
     3061 atacccagtt catacatttc attgcccatg atgccactta caacaccatg gaatcaacag
     3121 gaagtcttgc attgctggat gacgcctccc tgaagatcgc catcacgagg cactatgaca
     3181 gttaccaatc gctctacgat gagaacgagc gtatagagaa tttttccagg tcttactatg
     3241 ctacctacat gatggagcat gtggaccaga tggagttgtt catgtacggc aaaccctgtt
     3301 gttttgacga tacccgtttc cgcaacctgg tattcagcat gaccggtatc ctgcagatac
     3361 aggaaaagac ctaccgggaa gcactggaga gatgccgtga cctgatcagc aagctggagg
     3421 tttaggtctg catgatgaaa aatgccagca tagcgatgcc gaacagcaac aaagctgctg
     3481 caatgatgat ggctttagat atggccgcat tataagcagg tggtttcatt tctctctttg
     3541 aaatgagccc gaacttatcc atgatcacat gaatttgcgg tacatcatct tcatagacac
     3601 atacgaccgg gatacccggt tgttccaact taagatacgg tattgaagcc tgctgcagtt
     3661 cgcggatcag ggtggtcatg cccggctctt ccatggtgat gcgaaatccg gaaacagcga
     3721 ggtcttccag cgactgacgt tccagttctt cgggtggtat ggtgtccttt tctgaatctt
     3781 ccgtttcggc tatgatgcct ttgcgaatga aatactccat aaagtattca tgccatccct
     3841 ttttatcggc aatatcaaac ccggtactcc atatcaggcc gttctcttct gtatcctccc
     3901 ctgtggccgc ctgttccatg atgtggatca gttcatcgcc gttgagggcg tttagtttat
     3961 cgaataaagg gttatcg
//
LOCUS       DAOMAX010000242         3951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4284_length_3951_cov_4.346509, whole genome shotgun sequence.
ACCESSION   DAOMAX010000242 DAOMAX010000000
VERSION     DAOMAX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_3951_cov_4.346509"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..564)
                     /gene="iscU"
                     /locus_tag="PK742_10870"
     CDS             complement(145..564)
                     /gene="iscU"
                     /locus_tag="PK742_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HQU77135.1"
                     /translation="MAYSQKVLDHYENPQNVGTLDAKKETVGTGLVGAPECGDVMRLQ
                     IEVDPETHTITDAKFKTFGCGSAIASSSLATEWLKNKTIDEALQIDNMDIVEELALPP
                     VKIHCSVLAEDAIKAAIQDYRKKQGLPELKLEEKIVH"
     gene            complement(600..1817)
                     /locus_tag="PK742_10875"
     CDS             complement(600..1817)
                     /locus_tag="PK742_10875"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HQU77136.1"
                     /translation="MHNLPIYLDNSATTPVDPRVLEAMLPYFTQHFGNAASRSHPFGW
                     EAEDAVDLARQQVAKLINASDKEIIFTSGATEANNLAIKGAFDMYNRKGNHIITVTTE
                     HKAVLDTCKHIEKMGGEVTYLKVDNQGNIDLNELEAAITDKTILVAVMYANNEMGTIH
                     PIRKIADIAHKKGALLFTDATQAVGKISVDVERDGIDLMAFSAHKMYGPKGVGALYVR
                     RKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKAAELCMHEMEEEGKRLAILRDK
                     LENALMGLEEAYVNGNPDSRLPQLTNISFKYVEGEGLMMAFNKHIAVSSGSACTSASL
                     EPSYVLKALGLGDDLAHSSIRFSLGRFSNEAQVDYTIEAVTTAVTKLREMSPLWEMFK
                     EGIDLDTIEWAEH"
     gene            2129..2536
                     /gene="mce"
                     /locus_tag="PK742_10880"
     CDS             2129..2536
                     /gene="mce"
                     /locus_tag="PK742_10880"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQU77137.1"
                     /translation="MRKIEHLGIAVKDLEASNELFKKLLGSEHYKVETVEREGVSTSF
                     FLIGDTKIELLESSREDTAISKFIDKRGEGVHHIAFAVDDIHAEMERLQGEGFQLLYP
                     EPLPGADGKLICFLHPKTTNGVLVELCMDKPAH"
     gene            complement(2537..>3951)
                     /locus_tag="PK742_10885"
     CDS             complement(2537..>3951)
                     /locus_tag="PK742_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HQU77138.1"
                     /translation="VEGQKEADIRLTVRKRPGDYGVSPLQAIQSTSDHDIYHLDNYDK
                     LADAPLMYGQPDTASIMVGGAQVQIGVYSPNGVIQAAAVRDNIKDLLHAQELYLGGKL
                     PVEKYAFIFYFTDTLGVSGGMGALEHMNSSVYFLPEMPLNEEAVDMLRDVCAHEFFHI
                     VTPLNIHSHEIADFNFIEPHMSEHLWLYEGQTEYAAHHAQVMAGLITPDEFLRRMQDK
                     IDMSQNHYNDSLSFTEMSRRCLDEYKPEYTNVYQKGALINMCLDIELRELSGGKYGTR
                     DLMRDLGKEYGMNRSFNDDELFDKIAALTYPEIRTFFAKYVEGNTPIPYEDYFARVGV
                     ILSAPEKEKKLSMGNVALNMMMGEEGPVVEVVSTDGMNAFGKELGYEVGDRLVSINGV
                     QISEVGPMQAFESFRTQTKAGDPVAIEVMRTNKKGKTKLVTLKGTAMEVEVNKPRQLV
                     VDPHAGSAENTLRSAWLTGS"
BASE COUNT          858 a         1060 c         1015 g         1018 t
ORIGIN      
        1 cctcgttgtc aaaatccatt ttatacgaca agccgctgca gcctcctccc actacagaaa
       61 tgcggatgaa atagtccggg cctatttccg cctcctgctt gagggtgtca acctttgatt
      121 tggcttcttc gctgatgaag atcattagtg cacgattttt tcttccagtt tcagttcggg
      181 cagcccctgc ttcttgcgat aatcctggat ggctgccttg atggcgtctt cagccagtac
      241 ggaacaatgg atcttcaccg gcggcagggc cagttcttcc acgatatcca tgttgtcgat
      301 ctgcagcgcc tcgtcaatgg tcttgttctt cagccattcg gtggccagag aagaagaggc
      361 gatggcagaa ccgcagccga aggtcttgaa cttggcgtcg gtgatggtat gcgtttccgg
      421 atccacttcg atctggagtc gcattacgtc gccgcattca ggagcgccca ccagaccggt
      481 tccaacggtc tctttcttgg cgtccagagt acccacattc tgcgggttct catagtggtc
      541 gagtactttc tgtgagtatg ccatgatatc ttatttaacg gttgtactat tcagttggtt
      601 caatgttcgg cccattctat ggtatccagg tcgatgcctt ccttgaacat ctcccacagc
      661 gggctcattt cgcggagttt ggtgacggcc gtggtcacag cttcgatggt gtaatccacc
      721 tgggcttcat tggaaaatct tcccaggctg aaacggatgg agctgtgtgc caggtcatcg
      781 cccaggccaa gtgccttgag cacatagctg ggttccagtg atgcggaggt acaggcagaa
      841 ccggaactca cggcgatatg cttgttgaaa gccatcatca gtccttctcc ttccacgtat
      901 ttgaaagaga tattggtcag ctgaggaagc ctgctgtctg ggtttccgtt cacgtaggct
      961 tcttccagac ccatcaaagc gttttccagt ttatcacgca ggatggccag gcgcttgcct
     1021 tcttcttcca tctcgtgcat gcagagctca gctgccttgc cgaagcccac gatgcccggt
     1081 acgttcagtg taccggaacg catgccgcgt tcatgtccgc caccgtccat ctgagcggtc
     1141 accttgaccc tggggttctt ccggcgcaca tacaaagcac ctacgccttt cggtccgtac
     1201 atcttgtgcg cagagaacgc catcaggtcg atgccgtcgc gttccacgtc cacagagatc
     1261 ttgcctacgg cctgggtggc atcggtaaag agcagtgctc ctttcttgtg ggcgatatca
     1321 gcgatcttac ggatggggtg gatggtaccc atttcgttgt tggcgtacat caccgcaaca
     1381 agtatcgtct tatcagtaat ggctgcttcc agttcgttca ggtcgatgtt gccctggttg
     1441 tccactttga ggtaggtcac ctcgccgccc atcttctcga tgtgcttgca ggtgtccagc
     1501 acggccttgt gctcggtggt caccgtgatg atatggttgc ccttccggtt gtacatatca
     1561 aaggcaccct tgatggccag gttattggct tcggtggcac cggaggtgaa gatgatctcc
     1621 ttatcggagg cattgatcag tttggctacc tgctgacggg ccaggtcaac cgcatcttcc
     1681 gcctcccagc cgaacggatg cgagcgactg gccgcattgc cgaaatgctg ggtgaaatag
     1741 ggcagcatgg cctccagtac ccgcggatct accggggtgg tggcgctgtt atcgaggtat
     1801 atgggcagat tatgcatggt ttcccttctt atttagacta attctaaaac acaaaagtat
     1861 aggaaaagtt gggaaaaggg gtgtttttaa ttggcaattt tcagctgggg gtaggaaaaa
     1921 ggcggaattc ggaaggcgga atgcgaaatg aacacaaaaa cgggcaacaa actaccttgc
     1981 ttcactttat tcgactgcca actgtcagcg acaaaggagc tgatcccgcg ctacgaatgc
     2041 atcgactgaa aggagatagt agtcgttatg cgggactcac tcctccgcat tccgcccttc
     2101 gcattccgct ttcccttacc ttcgctttat gcgcaagatc gaacacctgg gtatagccgt
     2161 aaaggacctg gaagccagca acgagctgtt taagaagttg ctgggaagtg agcactacaa
     2221 ggtcgagacc gtcgagcggg aaggggtcag cacctccttt ttcctgatcg gagataccaa
     2281 gatcgagttg ctggaatcaa gccgggagga cactgccatc agcaaattca tcgacaaacg
     2341 cggagaaggc gttcaccaca tcgcctttgc ggtggatgat attcatgcag agatggaacg
     2401 gctgcaaggc gaagggttcc agttgctgta tccggaaccc ctgcccggtg ccgatggcaa
     2461 actcatctgc ttcctccacc cgaaaacgac caatggcgtg ctggtggaac tgtgcatgga
     2521 caagcctgcg cactgatcag ctgcccgtca gccaggcgct gcgcagtgta ttttcagcgg
     2581 aacccgcatg gggatccacc acgagctgac gtggtttgtt gacctccact tccatggctg
     2641 ttcctttcag cgtcaccaat ttcgtcttgc cttttttgtt ggtgcgcatc acttcaatgg
     2701 ccaccggatc gccggctttg gtctgggtgc ggaaggattc aaaagcctgc atcgggccca
     2761 cctcactgat ctgaacgccg ttgatgctca ccagcctgtc gcccacctca tagcccagct
     2821 ctttgccgaa tgcattcata ccatcggtgc tgaccacttc taccacaggg ccttcttcgc
     2881 ccatcatcat gttcaaagcc acattgccca tactgagctt cttctctttt tccggtgcag
     2941 acaggatcac acccacccgg gcgaaataat cttcgtacgg aataggcgta ttgccttcca
     3001 catattttgc gaagaaagtg cggatctccg gataggtgag tgcagcgatc ttgtcaaaca
     3061 attcatcgtc gttgaacgag cggttcatgc cgtactcttt tcccaggtct cgcatcaggt
     3121 cacgggtgcc gtatttgcca ccagacaatt cccgtaattc gatgtcaaga cacatattga
     3181 tgagagcacc tttctggtac acattcgtgt attccggttt gtactcatcg agacaacggc
     3241 gactcatttc agtaaaagac aggctgtcgt tatagtggtt ctgcgacatg tctatcttat
     3301 cctgcatgcg acgcaggaat tcatcaggag tgatgagtcc tgccatcacc tgtgcatgat
     3361 gcgctgcata ttccgtctgt ccttcataga gccagaggtg ttcgctcatg tgcggctcta
     3421 tgaagttgaa atcagctatc tcatgagaat gaatgttgag cggggttacg atgtgaaaga
     3481 actcatgtgc acacacatcc cgcagcatat ccacagcttc ctcattgagt ggcatctccg
     3541 gaagaaagta cacactggaa ttcatgtgtt ccaatgcacc catgcctccg gatacaccta
     3601 aagtatcggt gaagtaaaaa atgaaagcat atttctcgac cggcaatttg ccgccgagat
     3661 acaattcctg tgcatgaagc aggtccttga tattatctcg gacagcagca gcctggatca
     3721 caccgttcgg gctgtacact ccgatctgta cctgcgcacc tcccaccatg atggaggccg
     3781 tatccggttg accatacatg agcggtgcat cagcaagttt gtcgtaatta tccaggtggt
     3841 agatgtcatg gtcagaggtt gattgaatgg cctgaagggg actcactcca taatctccgg
     3901 gacgtttgcg cacagtcagt ctgatatctg cttccttctg accttccaca t
//
LOCUS       DAOMAX010000243         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4308_length_3941_cov_4.232373, whole genome shotgun sequence.
ACCESSION   DAOMAX010000243 DAOMAX010000000
VERSION     DAOMAX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4308_length_3941_cov_4.232373"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PK742_10890"
     CDS             complement(<1..581)
                     /locus_tag="PK742_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQU77139.1"
                     /translation="MKAIRLWLWTGAFMVLVQVMLGGITRLTGSGLSITEWDVLMGAL
                     PPMNAEQWNEAFDQYKQFPQYELVNSDMDLPGFKSIFWWEYIHRNWARLIGLVFLIPF
                     LYFWFSGRLNKAMRNRFLVVFLLGGLQGFMGWIMVASGLIDKPWVSPYNLTLHLLLAL
                     LVFLYLIWLALSMRKKPVPTDHPVAAKHIRWVLG"
     gene            complement(868..1737)
                     /locus_tag="PK742_10895"
     CDS             complement(868..1737)
                     /locus_tag="PK742_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU77140.1"
                     /translation="MKKLVPTLLFSLISLALLANPTDSIFQYTLQLKSNTDQIDFNDR
                     ISLLYFVTDSIGDKNDYVVKIQGYCSRDNKLKKASAFAQERADAVKATLIDNGFFDYQ
                     IIYCDGKGGPDINEDKVWLTFFPRTMKKEQIAAYEQELARQARYVYVKDSLQAGDLQP
                     GKIYIMEKLTYEQGKRSFSRESLPYALNLARVLAADTTLAVEVRSHMCCPEINTPDGM
                     DIISKKKELSKNRARELCGFLVKKGGLTGTRLKPVGLGATEPLVEEKDDASKKKNDRI
                     ELVVTKYVPPAAK"
     gene            complement(1944..3824)
                     /locus_tag="PK742_10900"
     CDS             complement(1944..3824)
                     /locus_tag="PK742_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77141.1"
                     /translation="MTLKLVFACILAIPAKSQSPDILFQKNLGSTDNDWLHCIIPTKD
                     EGFILAGNTVSNAGGNKSEDAIGSRDAWIVKMNGRGEIQWENTIGGSGYDHLQNVRQT
                     QDGGYIAAIISGSGVSGDKSQPSFGDMDYWLVKLDAGGNVLWDVTIGSTQDDWLHIAV
                     PTPDGGCLLGGWSFSGIGGLKTDATLGFCDFWVVKTDSVGNIEWQKTLGGSSYDYLRD
                     IIVKENGHSLIAGYSASPAGPYKSEANKGDEDYWILELNEAGDVLWESVAGGSGADLL
                     ESIYQDHNGRILVAGRSYSNASFDKSEDSFGDFDYWPVMLDSTGNLIWEKTIGGDGLD
                     MLYDIAQLRDSSYVFSGHSRSGISGNKSTLNQGDFDYWLVGMDEYGNISWQMDIGGSG
                     ADNTESICRNRYGEIIIGGYSVSPASGDKTESGWGGYDFWLVKAGQNDCRSYKPHHLY
                     STPKPDATAQLSWRSNYGDPLSGYEIYYRITGSPLWTIETSSDKMIMLNDLTSGASYE
                     WKVRSVCPFTEDSYAESVLAYFTIPLRQTEPLISHTQLTIYPNPSDGHFAIQIKTNME
                     SLPARIIFYDLSGKVVYTETMYLHFGYAVFKTDNPLPTGIYQIEIYTPFNQTTGSLYL
                     TR"
BASE COUNT          999 a          945 c          918 g         1079 t
ORIGIN      
        1 cccagcaccc agcgaatgtg ttttgctgca accgggtgat cggtaggaac aggtttcttt
       61 cgcatggaaa gagccagcca gatcaggtac agaaacacca gcaaggccag caggagatgc
      121 agcgtcaggt tataggggct cacccagggt ttgtctatca gtccgctggc taccatgatc
      181 cagcccatga aaccctgcag accacctaaa agaaagacca cgaggaaccg gttccgcatg
      241 gctttgttca gcctgccgga gaaccagaaa tacaggaaag ggatcaggaa aacgaggccg
      301 atgagtcggg cccagttgcg gtggatatat tcccaccaga agatgctttt gaaaccaggc
      361 aggtccatgt cgctgttcac cagctcatat tgcggaaact gtttgtactg gtcgaaagcc
      421 tcgttccact gttctgcatt cattgggggc agtgcgccca tgagcacatc ccattcggtg
      481 atggacagtc cggaacccgt cagccgggtg atacctccca gcatgacctg taccagcacc
      541 atgaaggcac cggtccacaa ccacaggcgt atcgctttca taagacaaag gtatatggag
      601 agaaacaaga aggaaatgaa aagttcattt tctgcgatag gtaccgtcga aggctgtttg
      661 ccaggttacc ccgccatcag ttgatcgctc ccaaaacagt cgggaatggt ctctattcac
      721 tctgatatcg ccggggtaaa agtgctgggt ttgtacttat ctcctttgcc cggtcaggag
      781 tcaaaatcac agtgatgacc actatgaccc ttcaccagtc aggaaaggac tataaattaa
      841 gcaataattt atgcgaaata aacgctatta tttggctgcc ggaggaacat atttcgtgac
      901 caccaattct attctgtcat tcttcttttt ggaagcatca tccttttcct ctacaagcgg
      961 ttcggtcgct ccaagtccga caggtttcag acgggtaccg gtaagtccac ctttcttgac
     1021 caggaatccg cacagctcac gggcccggtt cttggacaat tcttttttct tgctgatgat
     1081 gtccatgcca tcgggggtat tgatctcggg acagcacata tggcttctga cctctactgc
     1141 cagggtggta tcagctgcca ggacccgtgc caggttgagg gcatagggca gtgactcacg
     1201 gctgaaactg cgttttccct gttcataggt cagcttttcc atgatgtaga tcttgcccgg
     1261 ctggaggtcg ccggcctgca gggaatcttt gacatatacg tatctggctt gtctggcaag
     1321 ttcctgttca taggcggcta tctgctcctt tttcatggtt ctggggaaaa aggtcagcca
     1381 aaccttgtct tcgttgatat ccggtccgcc cttaccatcg cagtagatga tctggtaatc
     1441 gaagaagcca ttgtcaatga gtgttgcctt gacggcgtca gctctttcct gtgcaaaggc
     1501 cgatgctttt ttcagtttgt tatcgcggct gcagtatccc tggatcttga ccacataatc
     1561 attcttatct ccaatgctgt ccgtaacaaa gtacagcaga ctgatgcggt cattgaaatc
     1621 gatctgatcc gtattggatt tcagctgcag ggtgtactga aagatggagt cggtcggatt
     1681 ggccagcaag gccagagaga tcagggagaa caggagggtg ggtactagtt ttttcatcgg
     1741 cattcaggta tggcaataaa ggtaagtttc ctgggtaaaa gatgggaatt tggggcagga
     1801 ggttggcagg gatttaacag ataaatcatt taaaacggat gggggtatat gtgacatgat
     1861 ttattcttgc ctttctggat atcagaaacc aactgtcagt cctgatgtac attctgcatt
     1921 ctgatggctg ccctggaatt cagttatctt gttaaataaa gggaacctgt tgtttggtta
     1981 aacggagtgt atatttctat ctgatatatt ccggttggta gtggattatc agttttaaac
     2041 accgcgtatc caaagtgtaa atacattgtt tctgtataaa caaccttgcc tgacaaatca
     2101 taaaagatga tccttgccgg caggctttcc atatttgttt tgatttggat ggcaaaatgt
     2161 ccgtcggacg gatttggata tatcgtcaat tgggtatgag aaatcaacgg ttcagtttga
     2221 cgaaggggaa tagtaaaata agccagcaca gattctgcat aactatcttc tgtgaaagga
     2281 caaacagagc gtactttcca ttcataactg gcgccggaag taaggtcgtt cagcatgatc
     2341 attttatctg agctggtttc tattgtccat agcggcgatc ccgttattct gtaatagatt
     2401 tcataccctg aaagagggtc cccatagtta gatctccaac tcaattgtgc agtagcatca
     2461 ggctttggag ttgaatacaa atgatggggt ttataactcc tgcaatcatt ttggccagct
     2521 ttcaccagcc agaagtcata gcctccccat ccggattcag ttttatcacc ggaagccgga
     2581 gacactgaat agcctccaat tattatttct ccatatctgt ttcggcagat tgattctgta
     2641 ttgtcagcac cggatccacc gatatccatt tgccaggaaa tgttgccgta ttcatccata
     2701 ccaaccagcc agtagtcgaa gtctccctga ttgagagttg acttattgcc acttattcct
     2761 gaacgggaat gaccgctgaa tacataagag ctgtcacgca actgagcgat gtcataaagc
     2821 atatcaaggc catcaccgcc gatcgttttt tcccagataa gatttcctgt tgaatcaagc
     2881 atgaccggcc agtagtcaaa atcgccaaag ctatcttccg acttgtcaaa tgaagcattg
     2941 gagtatgacc ggccggctac aagaattcgc ccgttatgat cctggtagat actctccagc
     3001 agatcagccc cactacctcc tgctactgac tcccaaagaa cgtcacctgc ttcattgagt
     3061 tccaggatcc aatagtcctc atcgcctttg tttgcttcgc ttttgtaagg gccagccgga
     3121 gatgcagagt atcctgcaat cagggaatgt ccattttctt taacaatgat atcacgcaga
     3181 taatcatagc tgctgcctcc aagtgtcttc tgccattcaa tgtttccaac gctgtctgtt
     3241 ttaaccaccc aaaagtcaca gaagcccagt gttgcgtctg ttttcagtcc tccgatacca
     3301 gaaaatgacc agccacccaa caagcagcca ccgtctgggg tgggtacggc tatatggagc
     3361 cagtcatctt gagtgcttcc aatggtaaca tcccataata catttccacc agcgtccagt
     3421 ttaaccagcc agtaatccat gtcgccgaaa gatggttggc ttttatcacc actaactcct
     3481 gaaccggaga tgatggctgc tatatatccg ccgtcctgtg tttgccgaac gttttgcagg
     3541 tgatcataac cactgccacc aatggtattt tcccattgta tctcgccccg gccattcatt
     3601 ttgacgatcc aggcgtccct gctgccaatg gcgtcttctg atttattgcc tccggcattg
     3661 gaaacagtat ttcctgccag aatgaatccc tcatctttcg ttggtatgat acagtgcagc
     3721 caatcattat ctgtactacc gaggtttttt tgaaataata tatccggact ttgcgatttg
     3781 gcaggaattg ccaatatgca ggcaaataca agttttaatg tcaacgtctt attaatgctt
     3841 atacgaggac tgccccgtac aagttaaaca ttgaagtatg aactgcgaaa ggtagccatc
     3901 atgagataca ggtcagatgg cccggggtcc ctgactgcgt c
//
LOCUS       DAOMAX010000244         3934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4319_length_3934_cov_4.736272, whole genome shotgun sequence.
ACCESSION   DAOMAX010000244 DAOMAX010000000
VERSION     DAOMAX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4319_length_3934_cov_4.736272"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PK742_10905"
     CDS             complement(<1..1050)
                     /locus_tag="PK742_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77142.1"
                     /translation="MRFRLFTAVFLLGFSHFSARSQTDSLPFSIRLEEATWPEWPGLH
                     SFAHGVYNQRWFMLYGRQGGMHGMIPPDPFPLDEANQSVRMFDPETGQQWERSIWELP
                     DSIAFPLRSTNPQFVQQDDRVYVMGGYGKDTTSEDFVTFATLVAIDLPLLETALESGG
                     DIAPAFRLFRDSLFYLCGGEAELFDSDVVLFGGHEFTGEYSEIGGIGFTQRYTNEVRR
                     FHLTDDGADISISDISITYDSLLFHRRDLNLGPVRYPDGSEGLAAWSGVFQYDINLPW
                     LSDLYYDGASWTEDTLTHLFNHYTCPLMPLYDSNTADMHTVLFGGISQYYFYEPDSAV
                     YEDLNVPFTDNISCIT"
     gene            1146..2264
                     /gene="dnaN"
                     /locus_tag="PK742_10910"
     CDS             1146..2264
                     /gene="dnaN"
                     /locus_tag="PK742_10910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQU77143.1"
                     /translation="MKFIVSTTSLLRNLQMVNGVISTSTVLPILEDFLFEISKNSLTI
                     SGTDLETSISTSLEIESKAEGKVAIPARILVDYLKTLPEQPLTISVNDDNYSVEITTD
                     RGKYKLNGENGSDFPRIPVAENTSELSMSTGILQKAINKSLFAVSNDELRPQMTGVYF
                     QLNNEGVTFVATDAHKLVRYKRTDTTVKDAASFIVPKKALNLLKGILPGEETQVQISY
                     NSSNAFFSFNNINLICRLIDARYPDYNAVIPTDNPNELLVNRADLLNTLRRVMIFSNK
                     TTHQVILKLTGNSLNVSAQDLDFSNEAKEDLDCQYSGEDMEIGFNARFLQEMLQAMDN
                     EEVKIELSTPNRAGILLPTESEEGEDLLMLVMPVMLSA"
     assembly_gap    2304..2403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2507..3412)
                     /locus_tag="PK742_10915"
     CDS             complement(2507..3412)
                     /locus_tag="PK742_10915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU77144.1"
                     /translation="MYGPPDRKALIRYLSHSEIDKERWDACIAGSLNALPYAYSHYLD
                     VVTGNCWDALILDEYAAVFPLPVRKKWGFRAVFQPFFCQQLGLFVTDAAQYDLLPEFL
                     RAIPKRFIRVYVQLNTRNSMPAADHRVTYQIPLQRDYASIAEQYGTIVRKNMKRIGNM
                     ELTHESSPDVPTFLRFLKENLGDKLRDLGPKDMHMLQALLQMLLERKEGFIRYALDES
                     GQRQGAVFLVHSHGMLIYLLAAHTPAGKKKSVMTYLIDSVLQEYAEKEKLFDFEGSMI
                     PGIARFYSNFGAREVKFPVFRKGIV"
     gene            complement(3382..>3934)
                     /locus_tag="PK742_10920"
     CDS             complement(3382..>3934)
                     /locus_tag="PK742_10920"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77145.1"
                     /translation="ELFADYEVDAWILHRDGIAEAEIFYTTDTAAGFWNTSPMTLADV
                     ASNTWTGFIPAQEDGAEVFYYIYAQATTGKEQVRPITAPDGFWHFRVGGAISNLEESH
                     DDLIAVWPNPVDDHVSIDLRDLRSADKWQLSLTEPSGKEVYAATAFGGQVIHLPLALL
                     PSGTYWISLRSGDACMARQIVKR"
BASE COUNT          962 a          998 c          976 g          898 t
ORIGIN      
        1 ggtgatgcag gagatgttat cggtgaaggg cacgttgaga tcttcgtaca cagccgaatc
       61 gggttcatag aaataatact ggctgatacc accgaacaac accgtatgca tgtcagcggt
      121 attgctgtca tacagaggca tgagcggaca ggtatagtgg ttgaacaggt gggtgagcgt
      181 gtcttcggtc caggatgctc cgtcgtaata gagatcgctc agccagggca ggttgatgtc
      241 gtactggaat acgcctgacc aggcagccag tccttcgctg ccatcgggat acctgacagg
      301 acccaggttg agatcacggc ggtgaaacaa cagactgtcg taggttatac tgatatcact
      361 gatgctgatg tccgcaccat cgtcggtcag atgaaacctc ctgacctcat tcgtgtatcg
      421 ctgggtgaac ccgataccac ctatctccga atactcgccg gtgaattcat gtccgccgaa
      481 cagcaccaca tcgctatcaa acaactccgc ttccccgcca cagagataga acaggctgtc
      541 gcggaagagg cgaaaggccg gagcgatatc acccccgctt tccagagcag tttccagcag
      601 gggcaggtct attgctacca gggtagcgaa ggtgacaaaa tcttcgctgg tggtatcctt
      661 tccgtatcct cccatcacat atacacgatc gtcctgctgt acgaactgag ggtttgtgga
      721 tctcagcggg aaagcgatgg aatcgggcag ttcccagatg ctgcgctccc attgctggcc
      781 ggtttccgga tcgaacatgc ggaccgactg gttggcctca tccagaggga agggatcggg
      841 tggtatcatg ccgtgcatgc ctccctggcg accatagagc atgaaccagc gctggttgta
      901 aaccccgtgc gcaaaggagt gcaatccggg ccattcgggc caggtggcct cttccaggcg
      961 aatgctgaag ggcagggaat ctgtctgcga cctcgcagaa aaatgggaaa atccaagcag
     1021 gaaaacggct gtaaacaggc gaaaacgcat gactcaaagg tacattcttg tgggtattta
     1081 ggctcctgcg aatgtctgtt tatatttacc tttgaccgaa tcaaaaaagg ccttaaaacc
     1141 gacgcatgaa attcattgtc agtactactt ccctgctccg caatctacag atggtgaacg
     1201 gggtgatcag caccagcacc gtattaccca ttctggaaga ttttctgttc gagatcagca
     1261 agaactctct gaccatttcc ggcaccgatc tggaaacttc catcagtacc tcgctggaga
     1321 tcgaatccaa agcggaggga aaggtggcta ttccggcccg tatattggtt gattacctga
     1381 agacccttcc ggaacaacca ctcaccattt cggtgaacga cgataattac tctgttgaga
     1441 tcactacaga tcgtggtaag tacaaactga atggtgaaaa cggcagtgat tttccacgca
     1501 ttccggtagc agagaatacc agcgagctga gtatgagcac cggtatcctg cagaaggcca
     1561 tcaacaagtc actgtttgcg gtgagcaatg atgagcttcg tccgcaaatg accggtgttt
     1621 atttccaact taataatgaa ggtgtaacat ttgtggccac cgatgcacat aaactggttc
     1681 gctacaagcg caccgatact accgtgaaag atgctgccag ttttattgta cccaaaaaag
     1741 cacttaacct tttgaagggc atcctgccgg gcgaagagac ccaggtgcag atctcctaca
     1801 acagcagcaa tgcttttttc agcttcaata atatcaacct gatctgtcgc ctgatcgatg
     1861 cccgctaccc tgattacaat gcggtcattc cgacagacaa tccgaacgaa ctgctcgtga
     1921 accgtgccga tctgctcaac acgctgcgcc gggtcatgat cttctctaac aagaccaccc
     1981 accaggtcat tctgaagctg accggcaaca gcctgaacgt atcagcccag gacctcgact
     2041 tcagcaatga agccaaggaa gacctcgact gccagtacag tggtgaagac atggagatcg
     2101 gtttcaacgc ccgcttcctg caggagatgc tgcaggcgat ggacaatgaa gaagtgaaga
     2161 tcgagctgtc cactccgaac cgtgccggta tcctgctgcc caccgaaagt gaggaaggtg
     2221 aagacctgct catgctggtg atgccggtga tgctgagcgc ctgagggtaa gttagcagtt
     2281 ggcggttttc agttggcagt ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnagttggc agtttagcgc tggtctattt gtctccatac tcaataccca atactcacta
     2461 cccactactc aatactcaat acccactact caatactcat tacccactac acaataccct
     2521 tcctaaacac cggaaacttc acctcccgtg caccgaagtt gctgtagaat ctggcgatgc
     2581 cggggatcat actcccttca aaatcgaata gcttttcctt ttctgcatat tcctgcaaca
     2641 cggaatctat gaggtaggtc atgacacttt tcttcttgcc tgcaggcgtg tgtgctgcca
     2701 gcaggtagat gagcataccg tgactgtgca ccaggaagac cgcaccttgt ctttgtccgc
     2761 tttcgtccag tgcatagcgg atgaaacctt ctttcctttc cagcaacatc tgcagcagtg
     2821 cctgcagcat gtgcatgtct ttcggaccca ggtctctgag tttgtcgccc agattttctt
     2881 tcaggaaacg caggaaggtg ggcacgtcgg gcgatgattc atgggtcagt tccatgttgc
     2941 cgatgcgctt catattcttg cgcacaatgg tgccatactg ttctgcgatg gaagcgtaat
     3001 cacgctgtaa tggtatctga taagttaccc gatgatcagc tgcaggcatg ctgttgcggg
     3061 tgttcagctg cacatagacc cggatgaatc ttttaggaat ggcccggaga aattcaggca
     3121 gcagatcata ttgagctgca tcggtgacaa acaatccgag ctgctgacaa aagaagggct
     3181 gaaagaccgc ccggaaaccc catttctttc tgacgggtaa tggaaacaca gccgcatatt
     3241 catcgagtat gagggcatcc cagcagttac cggtcaccac atccaggtag tgcgaataag
     3301 catagggcag cgcattcagt gatcctgcga tacaggcatc ccatcgctct ttgtcgatct
     3361 cgctatgtga gaggtaacgt atcagcgctt tacgatctgg cgggccatac aagcgtcgcc
     3421 tgagcgtaaa gatatccaat aggtgccgga gggcagaagt gcgagcggaa ggtgaatgac
     3481 ctgtccgcca aacgctgtag cagcatacac ttccttaccg gatggttctg tcagcgagag
     3541 ctgccacttg tctgcactac gcagatcacg caggtcaatg ctcacatgat catcgaccgg
     3601 attgggccat acagcgatga ggtcatcgtg tgattcttcc agattgctga tggcaccacc
     3661 tacacggaaa tgccagaatc catcgggtgc tgtaatgggt ctcacctgtt cttttccggt
     3721 tgtggcttgg gcatagatat aatagaacac ttcggcacca tcttcctgcg caggaatgaa
     3781 gccggtccag gtattggaag ccacatccgc cagggtcatg ggcgatgtat tccagaaacc
     3841 tgctgcagta tctgtggtat aaaagatctc tgcttctgca ataccatcac ggtgcagtat
     3901 ccaggcatcc acttcataat cggcgaagag ttct
//
LOCUS       DAOMAX010000245         3919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4342_length_3919_cov_3.986284, whole genome shotgun sequence.
ACCESSION   DAOMAX010000245 DAOMAX010000000
VERSION     DAOMAX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4342_length_3919_cov_3.986284"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1104)
                     /locus_tag="PK742_10925"
     CDS             complement(265..1104)
                     /locus_tag="PK742_10925"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQU77146.1"
                     /translation="MQLIARADEVALGHDTVKQALPIRNIDQMDPFLLLHHFNKDIEP
                     GGPGLDVAPHPHRGFEPITFLFDGAIHHRDSRGNEGFLNGGDVQWMTAGMGIVHSEAA
                     QKPWLENGGKMNLIQLWVNLPKKDKMIQPRYQDIKADSIPVVEKEGLTARVVAGTWEG
                     VTGPAETRTPVVAVEGRIQKGHKLELPVDTTHNAVLYLMDGFLQFNDKHQAGGGFVAI
                     FRQDGNSIQIEALQDSHFLLLTGEPLNEPVSSYGPFVMNTQTEIMEAIRDYQMGKMGM
                     LTY"
     gene            complement(1243..2502)
                     /locus_tag="PK742_10930"
     CDS             complement(1243..2502)
                     /locus_tag="PK742_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQU77147.1"
                     /translation="MKIIKILGAVVVLLIIVLLVGKNKGWFGGGGGLTVQTDSVAVRD
                     IAETVTASGKIYPVNEVKISAEISGEIVDLPVYEGMMVKAGQLLVRINPDLYESQVEQ
                     AEAAVNNAKANLATARSRKLQSEVNMENATIAFERSKKLFADKVISQAEYEQAELAYK
                     TAKAEIEIAKESVTGMEYTVKSAEATLRQMRDNYKRTAIYSPIPGTVFGLSKKAGEKV
                     LGTIQMSGDVLMSIANLNDMEVQVDVSENDVLRIDLGDTAEIEVDAYLDRKFKGIVTQ
                     IANSAGSGGLIQVATEQATNFKVKIRILPESYADLNADGKFPFYPGMSATADIRTNTE
                     KNVLTVPIQAVTTREDTSKAGETSIMVWTISNDSAVMRMVTTGIQDDSYIQILDGLKE
                     GDVVISGPYNTVATILNDGDKVQVEKE"
     gene            complement(2504..3841)
                     /locus_tag="PK742_10935"
     CDS             complement(2504..3841)
                     /locus_tag="PK742_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQU77148.1"
                     /translation="MKRLLFGSLLLWSAGIFAQQPMDLRTCVETAVANNLQIRQGAAN
                     VAANEFAVQQSRLSLLPNLNFSANYFWSFGRGIDYVTNTYVAQNFESNSFTLSSNMGL
                     YTGGIKANGIKKAGYDLETARLDQQSMIENIQLNTILAFLQVIYAEDQVEIAQNKKTV
                     STKQLSDARKLADAGSIPAGNLLTLEAQIASDELNLVNARNNVATAYLALKNILQMDP
                     SESLSIIQPGDGLLAEVLAAEMPELSEVIDAATRNLPGIQRFEYALKSAESQVKITAG
                     YGLPSLTLVGQMNTSYSNANIGIPGFEPDPYEVQIENNLGEVVGLTLSVPLFNNGQVA
                     INRQNAELGLMNVELQQQIAINDMKTTVTNAWTGLQSAALTYEAALRSLESAQLAYDF
                     AESRFQAGASNSLDYTTAVNNLAQAEISLSQAQYDYIFKRKVIDYYLGKPIEF"
BASE COUNT          918 a         1046 c          895 g         1060 t
ORIGIN      
        1 aagcagttcc aaagacacct ataagaacta cggaaacgac cctacctgtg atgattggga
       61 aagtgtagaa acgacagatt ctaccaccat taccacttcc tgcgtttcca tctgatactc
      121 cactttcctt catagtcgtg gattgacaaa aggccggcgt tagcgccggc cttttcactt
      181 atgtaagata aatcgtattc agacagctat cggaaccaat taccaatagc tacaaaccaa
      241 taactaaaac aaataactaa taactcaata agtcagcatc cccatcttac ccatctggta
      301 gtcgcggata gcttccatga tctccgtctg ggtgttcatc acaaaaggac cgtaactgga
      361 aacaggttca ttcagaggtt cgccggttag caacagaaaa tgactgtctt gtaaggcttc
      421 gatctgaata gaattgccgt cctgccggaa gatggctacg aatccacctc ctgcctgatg
      481 tttgtcgttg aactgcagaa atccatccat cagatataat accgcattat gggtagtatc
      541 gaccggtaac tccagtttgt gtcctttctg tattcgaccc tccacagcta ccacaggtgt
      601 tctggtctcc gccggtcctg tcacgccttc ccaggtgcct gctaccacac gggcggtcag
      661 tccttccttt tccactaccg gaatggaatc ggctttgatg tcctgataac ggggttggat
      721 catcttgtcc tttttcggca gattgaccca tagctggatg aggttcatct ttcccccgtt
      781 ttccagccag ggcttctggg ctgcttcgct gtggacgata cccataccgg ctgtcatcca
      841 ctgcacatca cctccattca ggaaaccttc gttgccacgt gaatcacggt ggtggatggc
      901 tccgtcaaac agaaaagtga taggctcaaa cccgcgatgc ggatgaggtg ccacgtccag
      961 tcccggacct ccgggttcaa tgtctttgtt gaaatgatgc agcaacagga acggatccat
     1021 ctgatcgatg ttcctgatgg gcaaagcctg cttcacagtg tcatgcccca gggccacttc
     1081 atctgcccgg gcaatcagtt gcacagttct gagttgggtc atagtgtgtc aacgccgggc
     1141 cgcaacagaa tgttcccgga aggaatgaaa ggcggaagac gaaagacgaa ggtcggaata
     1201 tggaatcgct atttcgaatt ccgtattccg accttcgtat tactactctt tctccacctg
     1261 caccttatca ccatcgttca atatggtagc aacagtatta tacggaccgc tgatgaccac
     1321 atcaccctct ttcaggccat ccagtatctg gatatagctg tcgtcctgga ttccggtggt
     1381 aaccatgcgc atgaccgcag aatcattgga gatggtccag accatgatgc tggtctcacc
     1441 ggctttgctg gtatcttctc tggtggtaac cgcctgaatg ggcacagtca gcacattttt
     1501 ctcagtgttg gtgcggatat cggccgtagc actcataccc ggatagaacg ggaatttacc
     1561 atcggcgttc aggtcagcat aagactctgg caggatgcgg atcttcacct tgaaattggt
     1621 agcctgttcg gttgctacct gtatcagtcc gccgctgccg gcagagttgg ctatctgggt
     1681 aacgatgcct ttgaatttcc ggtctaaata ggcatccacc tctatttctg ccgtatcgcc
     1741 caggtctatg cgcagcacat cattttcgct cacgtccacc tgcacttcca tatcattcag
     1801 gttggcgatg ctcatcagta catcaccact catctgaatg gtacccagta ctttttcacc
     1861 tgcctttttg ctcaggccga atactgttcc gggaataggt gaatagatgg cggtacgctt
     1921 gtagttatca cgcatctgac gcagcgtggc ttctgcactt ttgacggtat attccatacc
     1981 ggtaacactt tccttcgcaa tttctatctc tgctttcgcc gtcttatagg ccagttcagc
     2041 ctgctcatat tccgcctggc tgatgacctt atctgcaaat aacttcttgc ttctttcaaa
     2101 agcgatggtg gcattctcca tgttcacctc agactggagc ttcctgctcc tggcggtagc
     2161 cagattggcc ttggcattgt tgaccgcagc ttcagcctgc tccacctggc tttcatacag
     2221 gtcagggttg atgcgcacca gcaactgacc ggctttcacc atcataccct catataccgg
     2281 cagatcaacg atctcaccac tgatctctgc actgatcttc acttcattga ccggatagat
     2341 cttgccggaa gcagtgaccg tttcggctat atctctgacg gccacactat cggtctgcac
     2401 ggtcagtccg ccgccaccgc caaaccagcc cttgttcttt cctacaagaa gaacaatgat
     2461 cagcagtacg acaacagctc ctaatatctt gatgattttc atattaaaat tctatgggtt
     2521 ttccgaggta atagtcgatc accttgcgtt tgaatatata atcatactgt gcctgactga
     2581 gtgagatctc tgcctgtgcc aggttgttca ctgcggtggt atagtccaga gaattggagg
     2641 ctcctgcctg aaatctggat tcagcaaagt catacgccag ttgagcagat tccagacttc
     2701 gaagggccgc ttcataagtg agggcggcag attgcagtcc ggtccatgca ttggtgactg
     2761 tggtcttcat atcattgatg gcgatctgct gctgcaactc cacattcatc aggccgagtt
     2821 cagcattctg cctgttgatg gccacctgac cattattgaa cagtggaaca cttaatgtaa
     2881 ggccaaccac ttcacccagg ttattctcga tctgcacttc atagggatcg ggttcaaagc
     2941 ccggaatgcc aatgttggca ttggagtaag aggtattcat ctggcctacc agggtaagag
     3001 aaggcaaccc gtaaccggcg gtgatcttca cctgactttc tgcacttttc agcgcatact
     3061 caaaacgctg aatacccggc agatttctgg ttgccgcatc gatcacctca ctgagttcag
     3121 gcatttctgc tgcgagcacc tcagccagca ggccatcgcc tggctgaata atacttagag
     3181 attctgaagg atccatttga aggatgtttt tcagtgccag gtaagcagta gccacattgt
     3241 ttctggcgtt caccagattg agttcatcac tggcgatctg tgcttccagg gtcaggagat
     3301 tcccggccgg aatactgcct gcatctgcca gttttctggc atcagaaagc tgtttggtgg
     3361 acactgtttt cttgttctga gctatctcta cctgatcttc tgcatagatg acctgaagaa
     3421 atgccagtat ggtgttgagc tgtatgtttt cgatcatact ctgctgatcc agacgggcag
     3481 tctccaggtc gtacccggct tttttgatcc cgttggcctt gatccctccg gtgtataacc
     3541 ccatattgct ggacagtgta aagctgttgc tttcaaaatt ctgggctaca taagtgttgg
     3601 tcacataatc gataccacga ccgaaactcc agaaatagtt ggcgctgaaa ttcagattgg
     3661 gcagcagaga cagcctgctt tgctgcacgg caaattcatt ggcagccaca ttggccgctc
     3721 cctgacgtat ctgcaggttg ttggcaaccg cagtctccac acaagtgcgc agatccatag
     3781 gctgctgggc aaaaatgccg gcagaccaca gcagcaaact tccaaataag agtcgtttca
     3841 tgatcagctc aaaattacac aagatgcccg taacccctgc cattcagcct gtcatagtcc
     3901 gttatttaac aatgattaa
//
LOCUS       DAOMAX010000246         3913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4348_length_3913_cov_4.613271, whole genome shotgun sequence.
ACCESSION   DAOMAX010000246 DAOMAX010000000
VERSION     DAOMAX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_3913_cov_4.613271"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PK742_10940"
     CDS             complement(<1..935)
                     /locus_tag="PK742_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQU77149.1"
                     /translation="MEKSINISGGSFLIQPGDAENTFTPEDLNEEQRMIHDMALEFTD
                     NELFTRLDEIDNHEPGLVPSLLEKAGELGLLGVSVPEEYGGFAKDFNTGTVVTEIVGA
                     AHAFSVAYSAHTGIGTLPILYFGTEEQKQKYLPGLATGQLKASYCLTEPGSGSDALAA
                     KTKAVLNAEGTHYILDGQKMWITNAGFADVFIVFAKIDGKDFTAFIVERNTPGLSVGA
                     EENKMGIKGSSTCQVFFENCAVPKDAILGEIGRGHVIAFNILNIGRYKLSAAAMGGCK
                     RSLTNAIRYANERQQFGKSISSFGAIQYKLSEMAIR"
     gene            complement(981..1343)
                     /locus_tag="PK742_10945"
     CDS             complement(981..1343)
                     /locus_tag="PK742_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQU77150.1"
                     /translation="MQNFKKLEIWQQSMDVAKQVYLASDRLPNSERFGLVNQMRRAAV
                     SVPSNIAEGSGRLTAPDFRHFLAVALSSSNELTTQVILSEMLNYLPGDLSLDLQDKLD
                     KNQRMIYKFIQSVNRNIK"
     gene            complement(1408..2586)
                     /locus_tag="PK742_10950"
     CDS             complement(1408..2586)
                     /locus_tag="PK742_10950"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQU77151.1"
                     /translation="MTQEAYIVAGLRTAVGKAPRGAFRFVRPDDLAVDLLAQLMAKVP
                     ELDPARVEDLIVGNAVPEAEQGLQIGRMIAVRAFPKTTGGMTVNRYCASGVETIAIAT
                     AKIRAGMADCIVAGGVESMSLVPTVGWRTVLNFETAKEHPDYYIGMGLTAENVAREFK
                     VSREDSDAFSYESHQKAIHAIDNGYFKEGIAPITVKEVFVNEKGKRDERSYMVDTDEG
                     PRKDTNLEALAKLKPVFAANGVVTAGNASQTSDGAAFVVVMSERMVKELNLTPLARMV
                     SCATVGVEPGLMGIGPVAAIPKALDMAGMKLSDIDLIELNEAFACQALAVIREAGLDP
                     EKVNINGGAVALGHPLGCTGTKLTVQIMNDMKRLNKKYGMVTACVGGGQGIAGVIERL
                     "
     gene            complement(2625..2981)
                     /locus_tag="PK742_10955"
     CDS             complement(2625..2981)
                     /locus_tag="PK742_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQU77152.1"
                     /translation="MHDYKKLQVWQDAVDMAVLVYELTGAFPKAEQYGLTSQLRRSAV
                     SVPSNIAEGSGRNNPGEFDHFLGIAAGSSYELETQLIVSSRLGYSSDTEIDKLLDKLN
                     VNQKMIYKLKSKLVKR"
     gene            complement(3050..>3913)
                     /locus_tag="PK742_10960"
     CDS             complement(3050..>3913)
                     /locus_tag="PK742_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQU77153.1"
                     /translation="EAVNKSIAIAEQNHRGLVIGNDAANFSAGANIAMMLMLAIEQEF
                     DELDMAIRMFQQMTTRVRFSSIPVVVAPHGLTLGGGCEMTLHADVAVAAAETYIGLVE
                     VGAGLIPAGGGTKELALRASDKYFKGDIELPTLQEMTLNIAMAKVATSAREAFDLGLL
                     RKGIDKEVINLNRVISTAKAAAIELAEDGYTEPQPRTDVRVLGRTALGSFYTFIASMR
                     MAGYISEYDELIAKKVAYVICGGDLSQPTNVSEQYLLDLEREAFLSLLGERKTLERMQ
                     HILKTGKPLRN"
BASE COUNT          907 a         1035 c          881 g         1090 t
ORIGIN      
        1 cggatggcca tttcagacag tttgtactga atggcaccaa aagaagaaat ggactttccg
       61 aactgctggc gttcgttcgc ataacggatg gcgttggtga gggaacgttt acaaccgccc
      121 atggccgcag cgctgagttt gtaccgacct atattcagga tgttgaatgc aatgacatgt
      181 ccgcgaccga tctctcccag aatggcatct ttcggcacgg cacaattctc aaagaagacc
      241 tgacaggtgg atgagccttt gattcccatt ttattctctt cggcgccgac agacaaaccc
      301 ggtgtatttc tctccacgat gaaagctgtg aagtctttgc cgtcgatctt ggcgaatact
      361 atgaagacat ctgcgaagcc cgcattggtg atccacatct tctgcccgtc caggatgtaa
      421 tgggtaccct ctgcattcaa taccgctttc gtcttggcag ccagcgcatc agaaccacta
      481 ccgggttcgg tcaggcagta ggatgctttc agctgtcctg ttgccaaacc cggcagatac
      541 ttttgcttct gctcttctgt accgaaatac agaatgggca aggtaccgat accggtatgg
      601 gcagaatagg ccacagagaa agcatgggca gcacccacga tctcggtcac cacggtgcct
      661 gtgttgaagt cctttgcaaa tccaccgtat tcttcgggaa ctgaaacacc caagagtcca
      721 agctcacccg ccttttccag cagcgatggt accagacccg gttcgtgatt gtctatttca
      781 tccaatcgtg tgaacaattc attatcagtg aactccagtg ccatgtcatg gatcatccgt
      841 tgttcttcat tcaagtcttc aggggtgaag gtgttctcag catccccagg ttggatgagg
      901 aaggatcctc cggatatatt gattgacttt tccattttgc tcgttttgcg ttattcgtta
      961 tgggttgttc gttttgttta ttatttgata tttcgattga ccgattgaat aaatttgtat
     1021 atcattcttt ggttcttatc aagtttgtcc tgcagatcaa ggctcagatc tcctggtaag
     1081 tagttcaaca tttcagacaa gattacctgg gtggtaagtt cgttggagga tgacaatgcc
     1141 actgcaagaa agtgcctgaa atccggagct gtcaacctgc cagagccttc agctatgtta
     1201 gatgggacac ttacagctgc cctcctcatt tgatttacca atccaaatcg ttcagaattt
     1261 ggcaggcgat ctgaagccaa gtagacttgt ttagcaacgt ccatactctg ttgccagatt
     1321 tcgagttttt tgaaattttg catataccaa tatttgaggg ttaagaattt ctattacgat
     1381 caacgcaata cgaacaacga ataacgacta cagtctttca atgacccccg ctattccctg
     1441 gccaccaccc acacaggctg ttaccatgcc gtacttcttg ttcagtcttt tcatgtcatt
     1501 catgatctgc acggtcagtt tggtaccggt gcaccccagc gggtgaccca atgccacggc
     1561 acctccattg atgttgacct tctccggatc caggccggct tctctgatga ctgccagagc
     1621 ctgacaggca aatgcttcgt tcaattctat cagatcgata tcactgagtt tcatgcctgc
     1681 catatccaag gctttcggaa tggctgccac cggaccgatg cccatgagac ccggttccac
     1741 tcctactgtt gcgcagctga ccattctggc gagcggagtc aggttcagtt ctttcaccat
     1801 gcgttcactc atgaccacca cgaaggcagc accatcggag gtttgtgagg cattaccggc
     1861 tgtgaccaca ccattggctg caaagaccgg cttcagcttt gccagtgctt ccagatttgt
     1921 atccttacgc ggcccttcgt ctgtatccac catataactt ctttcatcac gcttaccttt
     1981 ctcattcaca aaaacttcct tcacggtgat gggggcaatg ccttccttga agtatccgtt
     2041 atcgatggca tggatagctt tctgatgcga ctcataagag aacgcatctg aatcttctct
     2101 cgacaccttg aactcacggg ccacattctc agcggtcagt cccatgccga tgtagtaatc
     2161 cggatgttct ttagcggtct caaagttgag gaccgttctc cacccaaccg tgggtaccag
     2221 actcatagac tccactccgc cggccacgat gcagtctgcc atgcctgcac ggattttggc
     2281 agtagcgatg gcaatggtct ccactccact ggcgcagtag cgattcaccg tcatgcctcc
     2341 tgtagttttc ggaaatgccc gcacagctat catgcgaccg atctgcaatc cctgctccgc
     2401 ttccggcact gcatttccca cgatcaggtc ttctactctg gcgggatcca gttcgggcac
     2461 tttcgccatc agctgagcca gcagatcaac cgccagatca tcaggacgca caaaacgaaa
     2521 ggcaccacga ggggcttttc ctactgcggt tctgaggccg gctacgatat aagcttcttg
     2581 tgtcatagta ttgagtattg agtagtgggt agtgagactt gctcctacct cttcactagt
     2641 tttgatttta gtttgtagat catcttttga ttgacattca acttatcaag aagcttgtca
     2701 atctcagtat cagagctata acctaatctc gaagaaacga tcagctgagt ttcaagctca
     2761 tatgatgaac cagcagcaat tcctaaaaaa tgatcgaatt ctccgggatt atttcttccg
     2821 ctgccctcag caatattgga aggaactgaa acagcagatc ttcttaattg gcttgttaat
     2881 ccatattgct ctgctttagg gaaggctcct gtcagttcat aaaccaatac tgccatgtca
     2941 acggcatcct gccatacttg tagttttttg taatcgtgca tatatttcat ttgagggtta
     3001 agaatctttt ttagctatct cactacacaa tacccactac tcattaccat taattcctaa
     3061 gcggtttccc tgttttcaaa atatgttgca tgcgttcgag tgttttcctc tcgccgagca
     3121 atgagaggaa tgcttcccgt tccagatcga gcagatattg ttcacttaca ttagttggtt
     3181 gcgacagatc accaccacaa atgacataag ctactttctt ggcgatgagc tcatcatact
     3241 cgctgatgta gccggccatg cgcatagaag cgatgaaggt gtagaaggag cccaaagctg
     3301 ttcttcccag tacgcgtaca tccgttctgg gttgcggctc ggtatatcca tcttccgcca
     3361 actctatggc agcggctttg gcagttgaga tgacacgatt caggttgatg acttccttat
     3421 ctatcccctt tcggagcaat cccagatcaa aagcttcccg ggcggaggta gctactttag
     3481 ccatggcaat attgagtgtc atctcctgca aggtgggcag ctcgatgtct cccttgaaat
     3541 atttatcgct ggcacgcaag gcgagttcct tggtgccgcc accggcagga atcagtccgg
     3601 cacccacttc caccagacct atataagtct ctgctgctgc cactgccaca tctgcgtgca
     3661 gggtcatctc gcatccacca cccagcgtga gtccgtgcgg agctaccact accgggatcg
     3721 aagaaaagcg cactctggtg gtcatttgct ggaacatacg gatggccatg tccagttcat
     3781 cgaattcctg ttcaatggcc agcatgagca tcatggcgat gttggctcct gcactgaagt
     3841 tggctgcgtc gttaccgatc accagtccgc ggtggttctg ctctgcaata gcgatgctct
     3901 tattgaccgc ttc
//
LOCUS       DAOMAX010000247         3892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4389_length_3892_cov_3.602554, whole genome shotgun sequence.
ACCESSION   DAOMAX010000247 DAOMAX010000000
VERSION     DAOMAX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4389_length_3892_cov_3.602554"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PK742_10965"
     CDS             <1..1073
                     /locus_tag="PK742_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77154.1"
                     /translation="PNVTGAAKDRLAIVTAINAATPKITVKFLQAAWTTSAMSSGAYL
                     VLTTAAFSAGTGQPTSTIRNTRKKSAKLQIIKETWTGEGSEMTNGKWFKTTSGGKDIS
                     LFYAYGMEQVDYLHLMKTDGALLFGQETDNTITDPTGYSSNPISTTEGAFPKVRTEGN
                     TLEYPRGAFSIADFNEASQYCVNEKTGNAMMVWHGHKLGIELEDLLINYTSDTNINYV
                     VGETNGKKMLEFGFHSIKKAGITWVFRELDLLNNPEQFGVTGFEDKQHQAIVFPIAME
                     RMYANVERSSSRMAPLFGMRYKALGPYNRRMQVWEVGGTGHTPAARMITEYDTTNLYS
                     RSHIGFQGMCFNKWQIWEGVAA"
     gene            1142..2149
                     /locus_tag="PK742_10970"
     CDS             1142..2149
                     /locus_tag="PK742_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77155.1"
                     /translation="MRLLIDDVEQVVGLDMINRRFNTNFKPEGIVTFRLGPQFIKPDK
                     DNDGKLLMPRTYRICPKQTLVNEKGKEEHMFYYSSVSRSTNKDGRTVNTYISDLDFKN
                     GEKVIDLTPGSQNRDYAALYYLMACPRRAKDSGSGGLFYLYDEEFEEKVKAETARAEA
                     KIRGMIYDEDHKAYIDNEEVLALCRFFMIPGATVNSPEKNRNLLFAIAEQDPIKFLSE
                     RGSTKNNEYAQVVAEATEFGVLYYNPEESTYHYCRIEKDVRFAERTPIYQVGFAHKPR
                     PNVGFANWLILDDRDGHYDQICELLKAEKERLVAVYAHKGETYGVNNVKPMKFSYQME
                     Q"
     gene            2146..3744
                     /locus_tag="PK742_10975"
     CDS             2146..3744
                     /locus_tag="PK742_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77156.1"
                     /translation="MTATIVFDLDSESASFEKIVLTNTTSFADPDNVIGYWRITGPTG
                     VIHEGSYSSPDFTGGDPELEVAIPKLSSVPEYLLGGYTFVCYAKNTDTDEVYDALTIT
                     YTLASPSDDVMSSTGEIENACMDKTVNYFCQKITAEDTTNYGDYDTIDRDITLHPPSQ
                     ADEADTVVSNADTLTYYFAWVNAGYELHIDTLLEYVDSSVTLKIRLTYDDYFVVQNQK
                     LLADLYKGFNCYYNKFADIMCQEGGFNQVPAMWVANMSSLAFGVQAYEGAMKLGDWAQ
                     VEKYFDNLTAIIRRAGCYTCSCETQKPTLADPFCEGGVGSGSGTSYNFIAGSGMTISN
                     SGGNITIGLTTALYNFITGLFNDTVESADGSVVVTPTEDGDDKTWDLSVKNSFRFLAT
                     TSYSGGDSNIGITISGESREGNRYVNPSGAWKTDADNVQVVGYPFASNAALRVAPAVF
                     YIKDFLTVSGAVTDKVDIHARFLNAGATATDYTQTNKYWISWKNIDTNTIRLQVFDTN
                     GLPISMSQFVDDLTSMVLSVKINE"
BASE COUNT         1029 a          978 c         1034 g          851 t
ORIGIN      
        1 tcccgaacgt gaccggcgca gctaaggatc gtctggcaat cgtgaccgcg atcaacgctg
       61 ctaccccaaa aatcaccgtg aagttcctgc aagctgcatg gacgacttct gcaatgagtt
      121 ccggagcata cctggttctg accactgccg cgttcagcgc aggaactggc cagcctacca
      181 gtaccatccg taacaccaga aagaagtctg ccaagctgca gatcatcaag gagacctgga
      241 ccggtgaagg ttcggagatg accaacggta aatggtttaa gaccacatca ggtggtaagg
      301 atatcagcct tttctatgcc tacggtatgg aacaggtaga ttacctccat ctgatgaaga
      361 ccgatggcgc cctgttgttc ggtcaggaaa ccgacaacac catcactgac ccgaccggtt
      421 attccagcaa ccccatttct acgactgagg gtgcattccc gaaagttcgt acagaaggta
      481 ataccctgga gtatcctcgt ggcgcattct ctatcgctga cttcaacgag gccagccagt
      541 actgcgtgaa cgagaagacc ggaaacgcta tgatggtgtg gcatggccat aagctcggta
      601 tcgagctgga ggacctgttg atcaactaca ccagcgacac caacatcaac tacgtcgtag
      661 gcgagaccaa cggcaagaag atgctggagt tcggcttcca ctccatcaag aaggccggta
      721 tcacctgggt gttccgtgaa ctcgatctgc tgaataaccc ggagcaattc ggcgttaccg
      781 gcttcgagga caagcagcac caggctatcg tgttcccaat cgcgatggag cggatgtacg
      841 ctaacgtaga acgttccagc agccgtatgg ctccgctgtt cggtatgcgt tacaaggctc
      901 tcggcccgta caaccgccgt atgcaggtgt gggaagtggg cggtactggt catactcctg
      961 ccgcgaggat gatcaccgag tacgatacca ccaacctgta cagccggtca cacatcggct
     1021 tccagggtat gtgctttaac aagtggcaga tctgggaagg cgtagccgcc taaccgattt
     1081 aggatatgga gagctccttc gggagccctc ctttttgctt ttaacctttg aaatgtaata
     1141 tatgagactg ttaatcgacg atgtagaaca ggttgttggt ctggacatga tcaacagacg
     1201 attcaatacc aatttcaagc cggaagggat tgttacgttc aggctgggcc cccagttcat
     1261 taagccagat aaggataatg acgggaagct gctgatgccc agaacgtatc ggatttgtcc
     1321 gaaacaaacc ctggtgaatg aaaagggcaa ggaagagcac atgttctact attcctctgt
     1381 atccaggagt accaacaaag acggacggac cgtgaatacc tatatttccg atctggactt
     1441 caagaatggc gagaaggtga tcgacctcac tccgggtagt cagaatcgcg attacgcagc
     1501 tctgtattac ctgatggctt gcccgaggcg ggcgaaagac agcggcagcg gcgggttgtt
     1561 ctacctctac gacgaggaat ttgaagagaa ggtgaaggct gaaacagctc gtgctgaggc
     1621 caagatccgg ggcatgatct acgatgagga ccacaaagcc tacatcgata acgaagaggt
     1681 gctggcgctg tgccggttct tcatgatccc cggcgcaacg gtgaactctc cggagaagaa
     1741 ccgcaacctt ttgtttgcga tagctgaaca ggatcctatc aaattccttt cagagcgtgg
     1801 ttcgaccaag aataacgagt acgctcaggt ggtagcggaa gcaactgagt ttggcgtgtt
     1861 atactataac cccgaagaaa gtacctacca ttactgccgg atagagaaag acgtccgttt
     1921 cgcagagaga acacctattt accaggttgg ctttgctcac aagccaaggc ccaacgtggg
     1981 ttttgcgaat tggctgatcc tggacgacag ggatggacac tatgaccaga tatgtgaatt
     2041 gctgaaagct gaaaaagaga ggctggtagc tgtttacgct cacaaaggcg agacctatgg
     2101 cgtgaacaat gttaagccca tgaaattctc atatcagatg gagcaatgac agcaacaata
     2161 gtgttcgatc tggacagcga aagtgcgtcc tttgaaaaga tcgttctcac caataccacg
     2221 agctttgcag atcccgacaa tgtaattggc tactggagga tcaccggtcc caccggtgtt
     2281 atccatgaag gcagctactc cagccctgat ttcaccggcg gcgatcctga attagaggta
     2341 gcgataccta aactcagcag cgttccggaa taccttttgg gtggttatac ctttgtctgc
     2401 tacgccaaga acaccgatac cgacgaggtt tacgatgctc tgaccatcac ctacaccctg
     2461 gcatcaccga gcgatgatgt catgtccagc actggcgaga tcgagaatgc atgcatggac
     2521 aagaccgtga attacttctg ccagaagatc acggccgagg ataccaccaa ttacggcgac
     2581 tacgacacca ttgacaggga tatcaccctg catccgccat cacaggccga tgaagccgat
     2641 acggttgtca gtaacgccga tactcttacc tattactttg cctgggtgaa tgccggctac
     2701 gagctgcaca ttgacaccct gctggagtat gtggacagtt cggtcacgct gaagatccgc
     2761 ctcacctacg atgattattt cgtggttcag aaccagaagc tgctggctga cctatacaag
     2821 ggcttcaact gttactacaa caagttcgct gatatcatgt gccaggaagg aggcttcaac
     2881 caggttcctg cgatgtgggt ggcgaacatg agttctctgg cgttcggcgt tcaggcgtat
     2941 gagggggcga tgaagctcgg agactgggcg caggtggaga aatactttga caacctcacg
     3001 gccatcatca ggagagccgg ttgttatacc tgcagctgcg aaacccaaaa gcctactctg
     3061 gcagatcctt tctgtgaggg tggtgtggga agcggaagcg gtacctctta caatttcatt
     3121 gccggaagcg gcatgaccat cagcaattcc ggagggaata tcaccattgg gctcaccacg
     3181 gcgctataca acttcatcac cggcttgttc aatgacacgg tagaaagcgc agacggatct
     3241 gttgtggtaa ctcctaccga agacggggac gacaagacct gggatctgag cgtgaagaac
     3301 agcttccgat tcctggctac caccagttac agcggtggcg actccaatat cggcatcacc
     3361 atcagcggcg aaagcaggga ggggaatcgg tatgtaaacc catctggagc ctggaagacc
     3421 gatgcagaca acgtgcaggt ggttggatac ccctttgcca gcaatgccgc attaagggtt
     3481 gcgccggcgg tattctacat caaggatttt ctcaccgtct ccggagcggt gacggacaag
     3541 gtcgatattc acgcccgctt cctgaacgca ggggctacgg cgacagatta tacacagacc
     3601 aataaatact ggatttcgtg gaaaaacatt gacacgaata ccatacgcct tcaggtcttt
     3661 gacaccaatg gattgccgat aagtatgagt caatttgtag atgatctcac cagcatggtg
     3721 ctgtcagtaa aaatcaacga gtaatggcat tcaatatagg tgctctcggc aatggtgtcg
     3781 ggaaaatata caggaagcgg aacagcagtt ctggaaatct ggacacatta ccgaatacac
     3841 cagatgtcgt agctgcttta ttggctgaag caatcagaca gaggcagctt cc
//
LOCUS       DAOMAX010000248         3887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4400_length_3887_cov_5.016962, whole genome shotgun sequence.
ACCESSION   DAOMAX010000248 DAOMAX010000000
VERSION     DAOMAX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_3887_cov_5.016962"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK742_10980"
     CDS             complement(<1..450)
                     /locus_tag="PK742_10980"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU77157.1"
                     /translation="MKRIPSFSAAGMFLLAALLMSAGLKPTYSPPSGTGVIKGTVTDG
                     NTGEPLPFTTVVIKGTQIGDIANEDGHFLIKDLGAGKFTLEFKLIGYEDKEYTLSLKD
                     GETLEVNMQLYSSQAELDEVVVVSEKESRKVRAENVMPAITLSTHDAS"
     gene            complement(580..1521)
                     /locus_tag="PK742_10985"
     CDS             complement(580..1521)
                     /locus_tag="PK742_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HQU77158.1"
                     /translation="MKNRITDLFQIRYPIIQAGMIWCSGWELASAVSNAGGLGIIGSG
                     SMYPDVLKAHVRKCKTATNKPFAVNVPMLYPDIDQHIQTIIEEKVPIVFTSAGNPKTY
                     TAALKEHGIKVVHVVSSVKFAKKVEDAGCDAVVAEGFEAGGHNGREETTTLCLIPMVA
                     DAVSIPVIAAGGIGSGRAMLACMALGAEGVQIGSRFAASEESSAHPHFKEAIVGAGEG
                     STELSLKQLTPVRLIKNAFYQQVKEAEERGASKEELQQLLGRGRAKKGMFEGDLEEGE
                     LEIGQVSAGIHNILPAAAILENIWQEYLLTKSRLQQT"
     gene            complement(1521..2528)
                     /locus_tag="PK742_10990"
     CDS             complement(1521..2528)
                     /locus_tag="PK742_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQU77159.1"
                     /translation="MKAIIPVAGVGMRLRPHTYSQPKPLIPVAGKPILGFIIDRLKQA
                     GFKEFIFVIGYLGEKIESFVKEEYPDITSHFILQHTREGLGHAIWLCRDLVQEDEGVF
                     IALGDTIFEADLEAVMQEPVSALGLKKVDDPRDFGVAEVDVEGMITRLVEKPTIPKSN
                     LALVGLYKIREAKSLFEALETNINNSYKTQGEFHLTDALMLLIKTGVKFRGFKVQNWY
                     DCGKKEILLETNAILMKKMGTTKHDQFAYDNTIIIDPVSIAHGCQISNSIVGPNVTLG
                     EGAVVNYSIIRDSIIGNYSNIDDVVLHHSVIGSDATIKGLSQSLSIGDNTEIDLSQSI
                     S"
     gene            2571..>3887
                     /locus_tag="PK742_10995"
     CDS             2571..>3887
                     /locus_tag="PK742_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQU77160.1"
                     /translation="MKANQWLKRFLIWRARHISKRNFLLLIAVVIGMAAGVASALLKW
                     FVHFWEEQLIHINRDFAFEYLYLIFPVIGIGLTILFVRLLNHGHLGRGISSILHAISH
                     KSSILPRDMMYSHVVTSGLTVGFGGSVGLESPIVVTGSAIGSNLGMLFHFNYRERTLL
                     IGCGVAAAISSLFNAPIAGFVFTLEILLLELAVSSFIPVLLASVAGAITTSALAGTEV
                     IFSSERIDPFRLDQIHLFLLLGAATGIISLFFIRVSNYSRRWFTGLQGSTRPFLYGGI
                     LLGLCIFVFPPLYGEGYSTIRALLLNDMDVIADQSFFGLKVQGTTAILLYLVALLLLK
                     VLAANLTQGAGGNGGMFAPSLFVGAAAGYAFALLMNNVGLGYHLNLATFTLVAMAGVL
                     CGVNHAPLTGVFFIAEITSGYELMIPLVIVAAISYVTVKYFEPHPMP"
BASE COUNT          833 a         1000 c          954 g         1100 t
ORIGIN      
        1 gctggcatca tgtgtgctaa gtgttatggc aggcatgaca ttttctgctc tgaccttacg
       61 gctttctttc tctgatacca ctacgacctc gtccagttct gcctggctgg aataaagttg
      121 catattcact tcaagggtct cgccgtcctt caggctgagt gtgtattcct tgtcttcata
      181 tcctatcaat ttgaattcca gggtgaattt gccagcacct aagtctttga tcaggaaatg
      241 gccgtcttca ttggcgatat cgcctatctg ggtgccctta atgaccaccg ttgtgaaggg
      301 cagtggttcg cctgtattac catcggtcac agtgcctttt atgacacctg ttccggaagg
      361 gggtgaatag gttggtttga ggcctgccga catgagcagg gccgccagca ggaacatgcc
      421 tgcggcggaa aaagaaggaa tacgtttcat attggtacag tttgatctga tatctcgtgg
      481 atgcacagat caggaagatt ccacagggga tgacaaaaaa aagacaattc tccgtttgga
      541 aatgaggaga cagcaatggg gtgctgacac cagcatggac taagtttgct gcagacgact
      601 cttcgtcaga agatattcct gccagatatt ttccaggatg gcagctgccg gtaatatgtt
      661 gtgtattcct gcactcacct gaccgatctc caattcacct tcttccagat caccttcgaa
      721 catgcctttt ttggcacgtc ctcgccccag taattgctgc agttcttctt tggaggcacc
      781 gcgttcttct gcctccttca cctgctgata aaatgcattc ttgataagcc gtacaggtgt
      841 cagttgtttc aatgatagtt cggtggaacc ttcgccggca cccacgatag cctctttaaa
      901 atgagggtga gcgctgctct cttcgcttgc tgcaaaacga ctgcctatct gcactccttc
      961 ggcacccaat gccatacagg ccaacatggc ccgaccgctg cctataccac ctgctgcaat
     1021 aacaggaata ctgaccgcat cggccaccat cggtatcagg cacagggtgg tggtctcttc
     1081 ccgtccgttg tgtccgccgg cctcaaatcc ctcagctacc actgcatcgc agcccgcatc
     1141 ttccactttc ttcgcaaact tcacactgct caccacatgt accactttga tgccatgttc
     1201 tttcagggca gcagtgtatg tttttggatt accggcactg gtaaatacga tgggtacttt
     1261 ttcttctatg atggtttgaa tatgctgatc gatatcagga tacaacatcg gcacattgac
     1321 cgcaaatggt ttgttagtag cagttttgca cttgcgcaca tgcgctttga gcacatcggg
     1381 atacatgctt ccgctgccta tgatacccag tccgccggca ttgctcacag cactggccag
     1441 ttcccagccg gaacaccaga tcatacctgc ctggatgatg ggatatctga tctgaaacag
     1501 gtcagtgata cgatttttca tcagctgatg ctttgcgaca gatcgatctc ggtattatcg
     1561 ccgatgctca ggctctggct cagaccttta atggttgcgt cacttcctat gacagaatgg
     1621 tgcaacacca cgtcgtctat atttgagtag ttaccgataa tgctgtcgcg gatgatgctg
     1681 tagttcacca cagcgccttc gcccagggtc acattcggcc ctacgatgct gttgctgatc
     1741 tggcatccat gggcgatgct gaccggatct ataatgatgg tattgtcata agcaaactga
     1801 tcgtgcttgg tggtgcccat ctttttcatc agaatggcat tggtttccag aaggatctcc
     1861 tttttgccgc aatcatacca gttctgcacc ttaaagcctc tgaatttcac accggtttta
     1921 ataagcagca tcaatgcatc ggtcagatga aattctcctt gtgttttata ggagttatta
     1981 atatttgttt caagtgcttc aaacagggac ttcgcctccc ggatcttgta tagtcccacc
     2041 agggccaggt tgctttttgg aatagtaggt ttctctacca gacgggtgat cataccttcc
     2101 acatcgactt cggccactcc gaaatcacgt ggatcatcca ctttctttag cccgagcgct
     2161 gagaccggtt cctgcatgac agcttccaga tcagcttcaa aaatggtatc gcccagggcg
     2221 atgaacaccc cttcatcttc ctgcaccaga tctctgcata accagatggc atggcccagt
     2281 ccttcgcggg tatgctggag gatgaaatgg gaggtgatgt cagggtattc ttcttttacg
     2341 aagctttcga tcttttctcc gagatagccg atcacaaaga tgaactcctt gaatcctgct
     2401 tgcttcaggc ggtcgatgat aaaaccaaga atgggttttc ccgccaccgg aataagtggt
     2461 ttaggctgag agtaagtatg tggtctcagt cgcatgccca caccggcaac ggggataata
     2521 gccttcatag cacaaatata caattgcagg gcataggcta acttcgcagc gtgaaggcaa
     2581 accagtggct gaaacgcttt ctgatctggc gagcccggca tatcagcaaa cgaaatttcc
     2641 tgctgctcat cgccgtggtg atcgggatgg ctgcgggtgt tgcctctgcc ttgctgaaat
     2701 ggtttgttca tttctgggag gaacagctga tacatatcaa ccgtgacttt gcctttgagt
     2761 atctctatct gatctttccg gtcataggta ttggtctgac cattttgttt gtgcggttgc
     2821 tgaaccatgg ccacctgggc agaggcatca gcagtatcct gcacgccatc agccataaat
     2881 cgtccatttt accgcgtgac atgatgtact cgcatgtggt taccagcgga ctgaccgttg
     2941 gttttggcgg aagtgtgggg ctggagtcgc ccattgtggt gaccgggtcg gccattgggt
     3001 ccaacctggg tatgctgttt catttcaact atcgtgaacg caccctgctc attggatgcg
     3061 gtgtggcagc tgccatctcc agtctgttca atgcccccat agccggattc gtttttacgc
     3121 tggagatcct tttgctggaa ctcgctgtgt ccagtttcat tccggtgttg ctggcatctg
     3181 tagccggagc cataaccacc agtgccctgg ccggaaccga ggtcattttc agctcagagc
     3241 gcattgatcc gttcaggctg gatcagatcc accttttttt gctgttggga gcagcaacgg
     3301 gcattatctc actgttcttc atcagggttt ccaactacag caggagatgg tttaccggtt
     3361 tgcaaggcag tacccggcca ttcctgtacg gaggtatcct gctcggtttg tgcatctttg
     3421 tttttcctcc tctgtatggt gaggggtaca gcaccatacg tgctttactg ctaaatgaca
     3481 tggatgtcat agcagaccag agtttcttcg gtttgaaggt gcagggaact accgccatac
     3541 ttttgtacct ggttgcgctg ctgctgctca aagtgctggc agccaacctg acccaggggg
     3601 caggaggaaa cggcggtatg tttgctccgt ctctgtttgt aggcgccgct gcaggctatg
     3661 cctttgccct gctgatgaac aatgttggcc tgggttatca cctgaacctg gccactttca
     3721 ctctggtagc catggcaggg gtattatgtg gtgttaacca tgccccttta acaggagttt
     3781 tcttcattgc tgagatcact tccggttatg aactaatgat cccgctggtc atcgtcgccg
     3841 cgatatctta tgtgacggtc aagtatttcg aaccccatcc gatgccg
//
LOCUS       DAOMAX010000249         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4499_length_3847_cov_4.046414, whole genome shotgun sequence.
ACCESSION   DAOMAX010000249 DAOMAX010000000
VERSION     DAOMAX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_3847_cov_4.046414"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PK742_11000"
     CDS             <1..454
                     /locus_tag="PK742_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQU77161.1"
                     /translation="DVFYQLASNHGIVEGIENMTAWLFRVARNRIIDTYRKKKPESID
                     TSGIGSDEEDGDSYLAQIGAISAVRDDDPDALYERSLVWETLYEALSELPEEQRLVFE
                     LHELESKSFNEISELTGVGVNTLLSRKRYAVLHLRERMRDLYEEMMER"
     gene            592..969
                     /locus_tag="PK742_11005"
     CDS             592..969
                     /locus_tag="PK742_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77162.1"
                     /translation="MNKVLKIFGFILLGVAFIFLMGYATMLLWNWLIPEIFSGPVITF
                     VQALGLLLLVKLLTGFGNWGGKGHKHHSYGHGKMHWKKKWEAKMAGMNDDEKEKFKEM
                     YYRRCGRVWDDKKEEQSSPEQAD"
     gene            complement(966..1646)
                     /locus_tag="PK742_11010"
     CDS             complement(966..1646)
                     /locus_tag="PK742_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQU77163.1"
                     /translation="MRIKCLILDDEPLAVRLMVTYAAKVPELEVVMSGTDPRTALSYL
                     ERHPVDLLFLDIQMPGMNGLEIIQLTGGQIPVILTTAYDQFAVKGFELDAVDYLLKPF
                     SFERFRASVDKYLQRHHLVEREDALLVRSAGQVHRIPLAHIIYLESLRDYVIIHTTDR
                     SVRTLQSLQSFAERLPESDFMRVHRSFIVSRAHLSYMERSAVVMGEKKIPIGATYRDA
                     VKRWFSQG"
     gene            complement(1636..2655)
                     /locus_tag="PK742_11015"
     CDS             complement(1636..2655)
                     /locus_tag="PK742_11015"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQU77164.1"
                     /translation="MPGKRTWRLIAILVFIQLYAVQLLSLSMPDQQRNWTSMFSLFDL
                     TQVIYTVVTILLTRVVFMRYYRPKKWLMLAFGLVLVVALFIVVRFFLEQGLTVWLFGK
                     KNYYDPTFPYYIIDNIQYAFVTMFPGVIVFFAEESARHKERSLALEVSRKEAEMQYLM
                     ASMNPHFLYNSLNNIYALVESGSSHAGDAILQLTALTRYAISENAALVPLGDELDKVH
                     QYIGLQSLRYDHPVTIQIRNEVDTSTKVPPFLLITLAENACKHGDLNGWPVSIHLFEQ
                     QGQMVVETRNRLGRSGSTDSRGGVGLSNIHRRLELLFPGQVLWKQQFTETEFSLSIQF
                     PLHAD"
     gene            2834..3694
                     /locus_tag="PK742_11020"
     CDS             2834..3694
                     /locus_tag="PK742_11020"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HQU77165.1"
                     /translation="MKSFIIILLALTIAGVAQAQQPDIDAILIGDQPRPAVAVVGTFH
                     FAYYNLDAHKTEDHMQVDILSPSAQQEVEALVQYIARFNPTKILVESGPNTGYLMRKY
                     EHWQAGEGMGKNEREQLGFRLMAQFELDTIYGIDAWPLVYQLYDGQDSNCIRPIVDSL
                     YEGWDFQSDEFWSRRYDVYYDKDDSLNLEMPLTDYFLWMNDRHVLERGYGAYLVGDFK
                     LGDTEGADALSMHWYNRNLRIYRNIQRLATSPDDRLLVIIGQGHACILDHLFRCSPEF
                     DYVPFTEIGQ"
BASE COUNT         1041 a          948 c          985 g          873 t
ORIGIN      
        1 ggacgtattc taccagctgg cctccaacca cggcatcgtg gaaggcatag agaatatgac
       61 cgcctggctg ttccgggtgg cgaggaacag gatcattgac acctaccgga aaaagaaacc
      121 ggaaagtatc gatacttcag gtatcggcag tgatgaagag gacggcgaca gctacctggc
      181 gcagataggt gccatatcag ctgtcagaga tgatgacccg gatgccctct atgaacgcag
      241 cctggtatgg gagacactct acgaagcact cagcgagctg ccggaagaac agcgactggt
      301 atttgaactg catgaactgg aaagcaagag cttcaatgag atcagcgaac ttaccggtgt
      361 gggtgttaac accctactga gcaggaagcg ctatgccgtg ctgcacctga gggaacggat
      421 gagggacttg tatgaggaga tgatggagag atagttggca gttaacagtt ggcggttggc
      481 agttcttacc gatagaacct gaccaaaaac taaaaaccaa taaccaacaa cgcataacgc
      541 acaacgatta acgataaacg ataaacgaat aacgaacaac gcattacgac tatgaacaaa
      601 gtattaaaga ttttcggatt catcctgctg ggagtggctt tcatcttcct gatgggatac
      661 gcaaccatgc tgctgtggaa ctggctcatt cctgagatct tcagcggacc tgtcatcacc
      721 tttgtacagg cactgggcct actgctgctg gtgaaactcc tgaccggttt cggcaactgg
      781 ggtggcaaag gacacaagca ccattcctac ggccacggca agatgcactg gaagaaaaaa
      841 tgggaagcca agatggccgg catgaacgac gatgaaaaag agaagttcaa ggaaatgtac
      901 taccgccgtt gcggccgggt atgggatgat aagaaagaag agcagtcctc tccggaacag
      961 gccgattaac cctgactgaa ccaacgttta actgcatccc ggtaggtagc ccctatcggg
     1021 atcttttttt ctcccattac caccgcactc cgctccatat aggacaggtg tgccctggac
     1081 acgatgaaag acctgtgtac ccgcatgaag tctgactcag gaagacgctc ggcaaaggat
     1141 tgcaggctct gcagggttct gacagatctg tcggtcgtgt ggatgatcac atagtcacgc
     1201 aggctttcca ggtaaatgat atgtgccaga ggaatgcggt gtacctgccc ggcagaacgc
     1261 accagcaacg catcctcccg ctcaaccaaa tgatggcgct gcaagtactt gtcaaccgat
     1321 gctctgaaac gttcaaagga aaacggttta agcaggtaat ccactgcatc cagctcaaac
     1381 cccttcacgg caaactgatc ataagcagtg gtaaggatga ccggaatctg tccgccggtc
     1441 agctgtatga tctccagtcc gttcataccc ggcatctgaa tatccaggaa gaggagatct
     1501 accggatgac gttccagata tgacaatgct gttcgtgggt cagtgccaga catcaccacc
     1561 tccagttcag gcaccttcgc agcataggtc accatgagcc tgaccgccag gggttcgtca
     1621 tccagtatca ggcatttaat ccgcatgcaa ggggaattgg atggataaag aaaattctgt
     1681 ttcagtgaac tgctgtttcc ataacacctg cccgggaaac agcaactcca gtcgccggtg
     1741 gatattagac aaacccaccc ctcctctgct gtcggtactg cccgaccgtc ctaaccggtt
     1801 acgggtttcc accaccattt gtccctgttg ctcaaacagg tgaatggata ccggccagcc
     1861 gttcagatcg ccgtgtttgc aggcattctc agccagggtg atgagcagaa acggcggcac
     1921 ctttgtggag gtgtcaacct cattcctgat ctgaattgtt accggatgat cgtaacgaag
     1981 cgactgcaaa ccgatgtact ggtgcacctt gtccagttca tcgcccagtg gaacaagtgc
     2041 cgcattctca ctgatggcat accgcgtgag tgcagttagc tgcaaaatgg catcgcccgc
     2101 atggctgctg cctgattcca caagtgcata aatattattg aggctgttat acagaaagtg
     2161 cgggttcata ctggccatca ggtactgcat ctccgcttct ttccggctca cttccaatgc
     2221 aagactgcgc tctttatgcc gggcagattc ttctgcaaag aaaacgatca ctccgggaaa
     2281 catggtcaca aaggcatact gaatattatc tatgatatag taagggaatg tggggtcata
     2341 gtaattcttt ttgccgaaga gccagaccgt gaggccctgt tccaggaaga aacgcacaac
     2401 tatgaacaat gcaaccacca gcaccaggcc aaacgccagc atcagccatt tcttcggcct
     2461 gtagtaacgc atgaacacca ctcttgtaag caggatggtc accacagtgt aaatgacctg
     2521 ggtgagatcg aacaggctga acatagaagt ccagttgcgc tgctgatcag gcatggaaag
     2581 actcagcaac tgtaccgcat acaattgaat gaataccaga atggcgatga gtctccaagt
     2641 tcgttttccg ggcatgcaac aaagaaatac aaatctttgt cagtgtcggg caaaccatcg
     2701 gccaacacta tgtatttgtc tgcaaacatc tgataactgt cagaacaggt tgttcgtcag
     2761 gcaaacaagg ttgtatgcag ataagatttt tcgccggaca agctccaccc tactttcgga
     2821 aaaaatcaat ccaatgaaat catttatcat catcctcctc gccctgacca ttgccggggt
     2881 tgcgcaagca caacaacctg acattgatgc cattcttata ggcgatcagc caagaccggc
     2941 cgtagctgtt gtgggcacct ttcattttgc ctattacaat ctcgatgcgc acaaaaccga
     3001 agaccatatg caggtggata ttctgtcgcc atcggcacaa caggaagtgg aggcgctggt
     3061 gcagtatatt gcccgtttca atcctactaa gatactggta gaatctggcc cgaataccgg
     3121 ttatcttatg cgtaaatatg agcactggca ggccggagaa ggtatgggga aaaatgagag
     3181 ggaacagctg ggtttccggc tgatggcaca attcgaactg gataccatat acggtattga
     3241 tgcctggcca ctggtttatc aattgtatga cggacaggat tccaattgca tccgaccgat
     3301 cgtagattca ctctatgagg gctgggattt tcaaagcgat gaattctgga gcagacggta
     3361 tgatgtatat tatgacaagg acgatagcct gaatctcgag atgccactga ccgattattt
     3421 cctgtggatg aatgaccggc atgtgctgga acggggttat ggcgcctacc tcgtgggcga
     3481 tttcaagctg ggtgatacgg aaggggctga tgcactgagc atgcactggt ataaccgcaa
     3541 cctgcgtatt taccggaaca tacagaggct ggctacttct cccgacgacc gcttactggt
     3601 catcattggt cagggtcatg cctgcattct ggatcatttg ttcaggtgtt cgccggaatt
     3661 tgactacgta ccttttacgg agatcggaca gtgatcagat ctggaacact acagagtccc
     3721 ctccgggtga cactgtgtgg acgttagttg ggagccctga ggtcccaatg agttatctgc
     3781 attcccaaga aagtccccac agagttacct gaccgacgaa ggagggaaag gaactctgtg
     3841 gattgaa
//
LOCUS       DAOMAX010000250         3837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4521_length_3837_cov_3.314913, whole genome shotgun sequence.
ACCESSION   DAOMAX010000250 DAOMAX010000000
VERSION     DAOMAX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4521_length_3837_cov_3.314913"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1414)
                     /gene="ahcY"
                     /locus_tag="PK742_11025"
     CDS             complement(98..1414)
                     /gene="ahcY"
                     /locus_tag="PK742_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQU77166.1"
                     /translation="MNTMTDKKLPYKVKDINLAAWGRKEIQLAEAEMPGLMALREEFG
                     ASKPLKGARIAGCLHMTIQTAVLIETLVELGADVQWSSCNIFSTQDHAAAAIAAAGVP
                     VYAWKGMTLDEYDWCIEQTLFFGNDNQPLNMILDDGGDLTNIVLDKYPELIPHVKGIS
                     EETTTGVHRLYERMAKGTLPMPAINVNDSVTKSKFDNKYGCKESLVDAIRRATDVMMA
                     GKVAVVAGYGDVGKGSAQSLRGAGARVLIAEIDPICALQAAMDGYEVKKMIDGVAEAD
                     IVVTATGNKDIITGEHFRRMKPNAIVCNIGHFDNEIDVAWLNDNYGDSKDEIKPQVDK
                     YTIDGKDIILLAEGRLVNLGCAMGHPSFVMSNSFTNQTLAQMELWNNNGSYENKVYVL
                     PKHLDEKVARLHLKKLGVELDVLSKEQADYIGVTVEGPYKPEYYRY"
     gene            1547..1960
                     /locus_tag="PK742_11030"
     CDS             1547..1960
                     /locus_tag="PK742_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HQU77167.1"
                     /translation="MYPPALVMPMKQELTSVGFEDLTTAEEVREAIQDMPGTLLVVIN
                     SVCGCAAGYARPGVRLSLQHSKTPDRLATVFAGFDMESVAEVRKLTLPYPPSSPSMAL
                     FKDGKLVHFIERHQIEGHNAAMVAENLMKAYDKHC"
     gene            2197..3603
                     /locus_tag="PK742_11035"
     CDS             2197..3603
                     /locus_tag="PK742_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77168.1"
                     /translation="MTRENIPLRGQLAYASGMLGWSTLTSAVAVMLVYFYQPPSESGL
                     VNLIPKIGVLGVVNAMSLIMITARLWDAVIDPVIAWFSDKSQHRLGRRIWFMRISLLP
                     ILVTCFFLFTPATRMESQANIWYLVFIQILFNLSVSLYIIPYNAMLPELGHSSILKLR
                     LATYQQLGFIIGWILAASCPALVDYFKSNGMEPFRAYQYAIWIVFSIGCLFLVFPAFF
                     VNEKRYCDGVPTSDSLFGNLIPVLKNRNFFLYLVSDFTYFISLTTMGTGALYYVTVLL
                     HLPERYGTIMIIIMVSLSVLFYPLVNWLGARVSKKVIVLISLIGLAFIFLYVFYLGNM
                     GMADSTEAILLSVVMAVPLAFLGILPPVILAEITQYDSIRTGQNREATYFAIRSLFIQ
                     FGQTLGLALFTILIGLEADKGTGRWLQEVFPNIPFEELGIRLTGIIGFSLCLLAALIF
                     LSFRYNKMAGKLREWEGE"
BASE COUNT          886 a          980 c          967 g         1004 t
ORIGIN      
        1 aaatacagtg aagtgaaacg aacaagcagt tgttatgcgg agacccaaaa aaaaacccgg
       61 ccgcaagcgg ccgggatgat atacagatcg tgttggatca gtaacgatag tactccggct
      121 tgtaggggcc ttctacggtc acacctatat aatctgcctg ctctttggag agtacatcca
      181 gttccacacc cagtttcttc aggtgcagac gggcaacttt ctcgtccagg tgcttgggaa
      241 gtacatagac cttgttctca taagaaccat tgttgttcca caactccatc tgtgccaggg
      301 tctggttggt gaagctgttg gacatcacaa agctcgggtg acccatggcg caacccaggt
      361 tgaccaggcg accttcagcc aggaggatga tgtccttccc gtcgatggtg tatttgtcca
      421 cctgaggctt gatctcatct ttggagtcac catagttgtc gttcagccag gccacgtcga
      481 tctcgttgtc gaagtgtccg atattacaaa cgatggcatt gggcttcata cgacggaaat
      541 gttcgccggt gatgatatcc ttgtttccgg ttgcggtcac cacgatatcg gcttctgcaa
      601 ctccatctat catcttcttt acttcgtaac catccatcgc cgcctgaagt gcgcagatcg
      661 ggtcgatctc agcgatgagc acccgtgcac cggctccacg cagcgactgg gcagaacctt
      721 ttcccacatc accataacct gctactacag caactttacc tgccatcatg acatctgtgg
      781 cacgacggat agcgtccacc agagattcct tacagccgta tttattatcg aatttactct
      841 tggtcacact gtcattcacg ttgatggcag gcataggcag ggtgcctttg gccatgcgct
      901 catacagacg gtgcacaccg gtagtggtct cttcactgat accctttaca tgcgggatca
      961 gttcaggata tttatcgagg acgatgttgg tcagatcgcc gccatcatcc aggatcatgt
     1021 tcagtggctg gttgtcattg ccaaagaaca gggtctgttc gatgcaccag tcgtattcat
     1081 ccagggtcat tcccttccag gcatataccg gtacgccggc agctgcgatg gctgcagcgg
     1141 catggtcctg ggtggagaag atgttgcagg agctccactg tacatcggca cccagttcca
     1201 ccagggtctc gatcagtaca gctgtctgaa tggtcatgtg gaggcatccg gcaatgcggg
     1261 ctcctttgag cggtttgctg gcgccaaact cttcgcggag ggccatcagg ccaggcattt
     1321 cggcttctgc cagttgtatt tcctttcttc cccaggcagc caggttgatg tctttcacct
     1381 tgtaaggaag cttcttgtcg gtcattgtat tcatgcttat gctaacaatt tgatgcaaag
     1441 ttactaaatc gggtacgcat gcttgaacca atccacaccc ttgttggttc atggactgaa
     1501 gcacaaatca taattttgtg tctgataaac gaaaaagaag acaactatgt atcctcctgc
     1561 tttagtaatg cccatgaagc aagaactgac ctctgtcgga tttgaagacc tgaccacagc
     1621 cgaagaggtt cgtgaagcca tacaagacat gcccggcaca cttttggtgg tcatcaactc
     1681 cgtatgcggt tgcgcagccg gctatgcccg ccccggtgtc agactgagtc tgcagcacag
     1741 caagaccccg gacaggctgg caacggtctt tgccggattc gatatggaat ctgtagctga
     1801 agtgagaaaa ctcaccctgc cctatccgcc gtcttctccg tccatggccc tgttcaagga
     1861 cggcaagctg gtgcacttca ttgaacgcca tcagatagaa ggtcacaacg ctgccatggt
     1921 ggctgagaac ctgatgaagg cgtacgataa gcactgttaa aaggcggaaa ccgaaattcg
     1981 gaaggcggaa caggtatcga atacctgact tgtcgggcat ggatcaaacc gaccgcacgg
     2041 atgctttgca tcgggtaaag tatcagaaat gcacattcat gcagatcatt tcggcgaccg
     2101 ttatctcaaa taccctgatc cttcagatca gtactatctt gtcaaatccg tgtttcgccc
     2161 ccagattatc ctgatcaagt ctatttttgt gccgtcttga ccagagagaa cattccactc
     2221 agagggcagc tggcctatgc atcgggtatg ctcggatgga gcacgcttac cagtgctgta
     2281 gcggtcatgc tggtctattt ttatcagcca ccatctgaga gcggactggt caacctcatt
     2341 ccgaagatcg gcgtattggg cgtagtgaat gctatgagcc tcatcatgat caccgcccgg
     2401 ttgtgggatg ctgtgatcga tccggtcatt gcctggttca gcgataaaag ccagcacagg
     2461 ctggggcgac gcatctggtt catgcgcatc tcccttcttc cgatcctggt gacctgtttc
     2521 ttcctgttca ctcctgccac ccgcatggaa agtcaggcca atatctggta tctggtcttc
     2581 attcagatcc tgttcaacct gtcggtctca ctgtatatca ttccttataa tgccatgctg
     2641 ccggagctgg gacatagttc cattctcaaa ctgcggctgg ccacctacca gcaacttggt
     2701 ttcatcatcg ggtggatact ggcagccagt tgtccggcac tggtggacta tttcaaatcc
     2761 aatggcatgg aacctttcag ggcctaccag tatgccatct ggatcgtgtt ctccataggt
     2821 tgtttattcc tggtatttcc tgccttcttc gtcaacgaaa aacgttattg cgatggcgta
     2881 cccaccagcg acagcctgtt cggcaacctg ataccagtat tgaagaacag gaacttcttt
     2941 ctgtatctgg tcagcgactt cacctatttc atttcgctga ccaccatggg caccggtgca
     3001 ctgtattatg taacggtact gctgcatctg cccgaacgtt atggcaccat catgatcatc
     3061 atcatggtga gcctgagtgt cttgttctac ccgctggtca actggctggg agcgagggtc
     3121 agcaaaaagg tcatcgtgct gatcagtttg ataggtctgg cattcatctt tctgtatgta
     3181 ttctacctgg ggaacatggg tatggccgac agtaccgaag cgatactgct gtctgtagtg
     3241 atggctgtac cactggcatt cctgggcatc ctgcctccgg tcatcctggc agagatcacg
     3301 cagtatgaca gcatccgcac cgggcagaac cgggaagcta cctactttgc cattcgcagt
     3361 ctgttcattc agttcggaca gacactggga ttggcattgt tcaccatcct tatcgggctg
     3421 gaagcggaca agggtaccgg gcgatggctg caggaagtct ttcccaatat tccatttgaa
     3481 gaactgggca tacgtctgac gggcatcatc gggttcagtt tgtgcctgct ggcagctttg
     3541 atcttccttt cgttcaggta taataagatg gcagggaagc tgagggagtg ggaaggggag
     3601 tgaaagtaag cagttggcga ttggcggtgg gcagttgtag aaaatcggag tgtggaggca
     3661 ggaatggggg tatagaaaca gacctagcga cctaaaatga tagagataga ttgcattaag
     3721 ttattcgttg tgaaaagcac atacgtctga tatgatcgtc atcaccaaat tctataatta
     3781 ataatgcgtc ccatctaatc agcaccacac tgctattttc tgtgtcaatc ttaaata
//
LOCUS       DAOMAX010000251         3829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4535_length_3829_cov_3.997880, whole genome shotgun sequence.
ACCESSION   DAOMAX010000251 DAOMAX010000000
VERSION     DAOMAX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4535_length_3829_cov_3.997880"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..206)
                     /locus_tag="PK742_11040"
     tRNA            complement(133..206)
                     /locus_tag="PK742_11040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Pro,seq:tgg)
     gene            complement(261..349)
                     /locus_tag="PK742_11045"
     tRNA            complement(261..349)
                     /locus_tag="PK742_11045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Ser,seq:gct)
     gene            complement(484..1920)
                     /gene="dnaA"
                     /locus_tag="PK742_11050"
     CDS             complement(484..1920)
                     /gene="dnaA"
                     /locus_tag="PK742_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQU77169.1"
                     /translation="MSSKNHEGVWADCLKIIKDNVNHQSFKTWFEPIEPVKLEDDVLT
                     IQVPSQFFYEWLEEHYVALLRKTIKRELGANARLEYRIVVENSSGSQTPTTIHYPNYN
                     SGFNKNPEVAAPLIMGNTIKNPFVIPGLKKVNIESGLNPNYNFDSFIEGDCNRLCRSA
                     GYAVAQKPGGTAFNPLVIYGGVGLGKTHLAQAIGNEVKQNFPNKTVLYTNAERFTNQF
                     IESLKNNSVNDFVHFYQLIDVLIIDDIHFFSNKAKTQDIFFHIFNHLHQENKQIILTS
                     DRPPRDLEGVEERLLSRFKWGLSADLQAPDFETRVAILEKKMYADGIELPDDVVEFVA
                     YNITNNIRELEGALVSLLAQASLNRKQIDLDLAKKIVKNFVKNMSREVSIDFIQKAVC
                     DYFEVPVEKLKEKTRKRAIVQARQLSMYLSKNFTKNSLKSIGKHFGGRDHSTVIHSCQ
                     AIQNLMDTDSKFKESVEDLTKKIQMSIS"
     gene            complement(2017..2937)
                     /locus_tag="PK742_11055"
     CDS             complement(2017..2937)
                     /locus_tag="PK742_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HQU77170.1"
                     /translation="MSSRRQFIRQTGIAMAMAGLGQWPDALYAATGEETVLTILHTND
                     MHSRIDPFPADDKRYGGQGGMSQRAALIKSFRQTAQHVVLLDSGDIFQGTPYFNYFGG
                     EPEFRLMSAMGYDAATMGNHDFDAGVEGFARQLPHADFPFLVSNYDFTNTALQGRTKD
                     YTVVKRGRIRIGIFGLGIELHGLVPDTLFGETRYLEPVSIAQQMSNILRYDERCDVVI
                     CLSHLGYRYEENKVSDQILAKSTGGIDLILGGHTHTFMEKPEALSNKDGEEVLINQVG
                     WAGLRLGHLELILNPVKRKKWRYETPVIVE"
     gene            complement(2930..3673)
                     /locus_tag="PK742_11060"
     CDS             complement(2930..3673)
                     /locus_tag="PK742_11060"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HQU77171.1"
                     /translation="MKTTLFRHILWLAIVLLMAASCSRYQVVSISSENMSIAGRDTLF
                     NSMISPYREQIYAEMNEVIGRVGSDMFKAQPEGELGNWVCDVLLEESEELFGTPADFA
                     VYNYGGLRIDMIAAGPLTQGKLFELLPFENYVVMCTLDAYSTARLLEKIASAGGWPVA
                     GIEMVIDSAGQPADVRIQGVPLRRDATYSVVMNDYMANGGDGMDFMTGQPVETAGVTV
                     RDLVIAHVRKQTAAGQVIGSSLENRIRIE"
BASE COUNT          944 a          987 c          901 g          997 t
ORIGIN      
        1 agaaagtggg gcagaaaaga cagaagcagt ggtgaatgac gttactgcta tgcccactga
       61 tcagtgagca gcagtatcta tatctattta caaaaacaac cgccacacct tgcggtgtgg
      121 cggtttgtat cgtcggggtg gcaggatttg aacctacgac ctcctgctcc caaagcaggc
      181 attctgccgg actgaactac accccgagcg gtgtatctga aacctgacct ttctcaagca
      241 aacgcttcaa aagatcagag cggtgaggga gggattcgaa ccctcggtac cagttgccca
      301 gtacgacagt ttagcaaact gttggtttca gccactcacc cacctcaccg tgctgggctg
      361 aacacctgtt tcagacagga aggcaaaggt aatatatctg tctcttcatt tgcaaggaat
      421 tagccgatcg caaaaagaaa agcggcctca agggccgctc gtctctgtaa atatggtcgg
      481 gtttcacgaa atgctcatct ggatcttctt ggtcagatcc tccacgcttt ccttgaattt
      541 actgtctgta tccatcaggt tctggatggc ctggcaggag tgaatgaccg tagagtggtc
      601 acgacctcca aaatgcttgc cgatgctctt gagtgagttc ttggtgaagt tcttgctcag
      661 gtacatgctc agctgacgag cctgcacgat ggcccgcttc cgggtctttt ccttgagctt
      721 ttctaccggc acctcgaaat aatcacacac tgctttctga ataaagtcta tgctcacctc
      781 acggctcatg ttcttcacga agttcttgac gatcttcttg gccaggtcca ggtctatctg
      841 cttgcggttg agagatgcct gagccaacag actgaccagc gcaccttcca actcccttat
      901 attattggtg atgttgtaag ctacaaactc aaccacatca tccggaagtt cgatgccatc
      961 ggcatacatc ttcttttcca ggatggccac ccgggtctcg aaatcaggtg cctgtaggtc
     1021 agcactcaat ccccacttga agcgactcag caaacgctct tccacgcctt ccagatcacg
     1081 gggtggacga tcgctggtaa ggatgatctg cttgttctcc tggtggaggt gattgaagat
     1141 gtggaagaat atatcctggg tcttcgcctt gttggagaag aagtggatgt cgtcaatgat
     1201 gagtacatcg atcagctgat agaagtgtac gaagtcattg accgaattat tcttcagtga
     1261 ttcgatgaac tggttggtaa atctttcggc gttggtgtac aataccgtct tgttcggaaa
     1321 gttttgtttg acctcatttc cgatcgcctg tgcaaggtgt gtctttccca gccccacccc
     1381 tccgtagata acgagtgggt tgaaggcagt gcctccgggt ttctgggcca cggcatagcc
     1441 ggcactacga cacaagcggt tgcagtcgcc ctctataaag gagtcaaaat tgtaattcgg
     1501 gttcaggcca gattcaatgt tcactttctt gaggcccgga ataacgaaag gatttttaat
     1561 ggtgttgccc atgatcaaag gtgctgctac ttcggggttc ttgttgaaac ccgaattgta
     1621 attgggataa tggatggtgg taggcgtctg agaaccggaa ctgttctcca ccacgatgcg
     1681 gtattccagg cgggcattgg cacccagttc tcttttgatg gtcttgcgca gaagggctac
     1741 ataatgctct tccaaccatt cataaaagaa ctgactgggt acctgaatgg tcaatacatc
     1801 gtcttcgagt tttacgggtt caataggttc aaaccaggtc ttaaaacttt ggtggttcac
     1861 gttgtcctta ataatcttca ggcaatccgc ccacacacct tcatgattct tgctgctcat
     1921 cagtcagtta aacttgcctt atcaaattat taacagaaat gggagacgaa agtgttgaaa
     1981 aaattctgca caaaaaaatt tttttggtct tgatttttat tcgacaataa caggtgtttc
     2041 ataacgccat ttttttcttt tcaccgggtt taaaatcaat tcaagatgtc cgagtcgtaa
     2101 gcctgcccag cccacctggt tgatgagcac ctcttctccg tccttattag acaaggcctc
     2161 cggcttttcc ataaaggtat gggtatggcc acccaggatc aggtcaatac cacccgtgct
     2221 tttggccagg atctggtcag acactttgtt ctcttcatac cggtagccca ggtgagacag
     2281 gcatatgacc acatcgcacc gttcatcgta acgcagtatg ttgctcattt gctgagcaat
     2341 tgagactggt tccagataac gtgtttctcc gaacagggta tcgggaacca ggccatgcaa
     2401 ttctatgccg agaccgaaga ttccgatgcg gatcctgcca cgtttgacaa ccgtatagtc
     2461 tttcgttctt ccctgcaggg ccgtattggt aaaatcgtag ttggatacaa gaaaggggaa
     2521 gtcagcatgc ggcaactgtc ttgcaaatcc ctcaacacct gcatcaaagt catggttacc
     2581 catcgtagcc gcatcatacc ccatggcact catcaacctg aactccggct caccgccaaa
     2641 atagttgaag tagggagtac cctggaaaat gtcgccactg tcaagcaaga ccacatgttg
     2701 tgctgtctgt ctgaaggatt ttatgagggc tgctctctgc gacatgcctc cttgtccgcc
     2761 atagcgttta tcatctgccg gaaaaggatc aatgcggctg tgcatgtcgt tcgtatgaag
     2821 tatggtcagc acggtctcct cgccggtagc agcatacagg gcatcgggcc attgcccgag
     2881 acctgccatc gccatggcaa tgcctgtctg tctgatgaac tgtcttctgc tactcaatgc
     2941 gtatcctgtt ttccaatgaa ctgccgatca cctgaccggc tgcagtttgt ttcctcacat
     3001 gtgcgatcac aagatccctc acagtcactc ctgcagtttc gaccggttgc ccggtcataa
     3061 aatccatacc atcgccaccg ttggccatgt agtcattcat gaccaccgaa taggtagcat
     3121 cccgtctcag aggaaccccc tgaatccgga catctgcggg ttgcccagca gaatcgatca
     3181 ccatctcgat gccggccaca ggccagccac cggcagatgc gatcttttcc aataaccgtg
     3241 cggtggaata tgcatccaat gtacacatta ccacataatt ctcaaagggt aacaactcaa
     3301 aaagtttacc ctgtgtgagt ggtccggcgg cgatcatgtc aatgcgcaac cctccataat
     3361 tatagacggc aaaatctgct ggtgtaccaa acagttcctc agattcttcc agcaatacat
     3421 cacagaccca gttacccaat tctccttcgg gttgagcttt gaacatgtct gaacccaccc
     3481 tgcctatcac ctcattcatc tccgcataga tctgttctct gtaaggagag atcatgctgt
     3541 tgaacaaggt atccctgcct gcaattgaca tattttcaga gctgatagag acaacctgat
     3601 aacgtgaaca ggaagctgcc atcaatagca caatagccag ccataggatg tgccggaata
     3661 aggttgtttt cacacttcaa attttggaaa aaaagcccgc cggttcccgg atcaggagag
     3721 tttcaggcgc aagatcgccg aagccggcat gccgaaggtg gtggaaacca aggccaaggc
     3781 cgaactcaac aagctcaagc agatgtcgcc gatgtcggcc gaggcggcc
//
LOCUS       DAOMAX010000252         3824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4546_length_3824_cov_4.610241, whole genome shotgun sequence.
ACCESSION   DAOMAX010000252 DAOMAX010000000
VERSION     DAOMAX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4546_length_3824_cov_4.610241"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2441)
                     /locus_tag="PK742_11065"
     CDS             complement(<1..2441)
                     /locus_tag="PK742_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQU77172.1"
                     /translation="MFERIISFSVKNKLIVLVAVLAMAAVGVDSLSRIPIDAVPDITN
                     NQVQVVTVSPTLAAEEVEQYVTYPIESYMANIPEVTELRSISRYGLSVVTIVFEDRTD
                     IMKARQFVGEQIQLAAAEIPSELGSPEMMPITTGLGEIYQYVLTVDSMHTGQYSDMDL
                     RTIQDWVIKRQLAGMEGIIEVSSFGGRVRQYEVSADPVRMLQWEVTLDDIFRALADNN
                     ANSGGSYLEQADKAFYIRMEGRAGSLADIGLIPVTVKGGVPVRIRDVATVGFGSPQRY
                     GAMTMDGQGEVVGGITLMLKGANSSEAIRNVKERIEEVEASLPEGVHIYPYLDRSELV
                     GRTIHTVSRNLIEGGLIVIFIVVLLLGNWRAGLVVASVIPLSMLFALIMMRIFGVSAN
                     LMSLGAIDFGIVVDGAVIIVESVLHYLYTFRRGSKLSRQQMDEAITFSASQIYRSAAF
                     GVLIILVVFVPIFTLEGIEGKMFLPMAQTVSFAVLGSLLLSLTYVPVISAMALDRNIR
                     EHHTFADRLVGALRKAYKPAITFAVRKPVMVIISSLAALVLAIILFLRMGAEFVPTLE
                     EGDLAMQMSVEPGSSLSHSIARSSEAERLLKQHFPEVLHVVSKIGTAEVPTDPMAIED
                     ADIMIVMKDRKEWTSADNREDLIGLMKTVLDTIGDASFEFTQPIQLRFNELMTGAKTD
                     IAVKIYGEDPVELKRIADEAAEMISSIHGAGDVKVEQTAGLRQWRVQMDRRQMSVHGI
                     DVASVNRAIRTAYAGEVAGSVYENERRFDLVVRFSESGRETLNLNRIMVPAASGELVP
                     LSQVAVVQEMLSPMQ"
     gene            2950..>3824
                     /locus_tag="PK742_11070"
     CDS             2950..>3824
                     /locus_tag="PK742_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77173.1"
                     /translation="MYVAAAPLVYTLLFLIWLQRKGYFGGLHARWWQGAFILHVLAGI
                     GNLAVWVFAVGHGDSINYIHDGHMIWETLRSHPSHFLELTFGWGADNVYPEHLLYISE
                     PLKYSWNNIEYTMVRIHAILFVFTFGNAWGNIVLLNIAYFAGARFLYFRLWQRHPERK
                     TGLFGLVLLIPSVVIWSAGTLKEGPALMLMMFISGLLLDMEKQFRWRSLFWLFVCMAV
                     MLLIREYYVIFLVPLLFLWWMGQKISRSGWLFFGAALAGMVVVLVADRTSDDYSVAEI
                     ICEAQQFYIPIGTDP"
BASE COUNT          939 a         1007 c          916 g          962 t
ORIGIN      
        1 atctgcatag gacttagcat ttcctgtaca actgctacct gcgacaaagg caccagttca
       61 ccacttgctg caggcaccat gatccggttg aggttcagtg tttcacgccc cgattcactg
      121 aatctgacca cgaggtcaaa acgacgttca ttttcataga cggatccggc cacctctcct
      181 gcataagcgg ttcgtatagc tctgttgacg gaggcaacat caataccatg caccgacatc
      241 tggcgacggt ccatctgtac cctccattgc cggagccctg ctgtttgttc taccttaaca
      301 tctcctgcac catgtatgct gctgatcatc tccgcagcct catcggcaat gcgtttaagc
      361 tccaccgggt cttccccgta gatctttaca gcaatatccg tcttggctcc tgtcatcagc
      421 tcattgaatc gaagttgtat cggttgtgta aactcaaacg acgcatcgcc gatggtatcc
      481 aataccgttt tcatcaaacc aataaggtct tcccggttat cagctgaggt ccattccttc
      541 cggtccttca tgacgatcat gatatcagca tcttcaatgg ccatgggatc tgttggtacc
      601 tctgcagtgc cgatcttgga taccacgtgc aacacttccg gaaaatgctg tttcagcaaa
      661 cgctcagcct ctgacgagcg tgcaatgctg tgactcaggg atgaaccagg ttccacagac
      721 atctgcatag ccagatctcc ctcctccagc gtaggaacga actctgcccc catgcgcagg
      781 aacagtatga tcgcaaggac caaagctgcc aatgagctga ttatgaccat gactggtttt
      841 cttaccgcaa aagtgatggc aggcttgtaa gcttttcgaa gtgcaccgac caggcgatcg
      901 gcaaaagtgt ggtgttcccg gatgttgcga tccagggcca ttgcagagat caccggcacg
      961 taggtaagtg ataacagcaa tgatcccagc actgcaaaac tgaccgtctg ggccatgggc
     1021 aggaacatct taccttctat tccttcgagg gtaaatatgg gaacgaacac caccagtata
     1081 ataagcaccc cgaaggctgc ggaacggtag atctgagaag cagaaaatgt gatggcctca
     1141 tccatttgct gccggctcag tttactgcca cggcggaatg tgtacaagta gtgaagcaca
     1201 ctttccacaa tgatgacagc gccgtccacc acgataccga agtctattgc acccaaagac
     1261 atcaggttgg ccgatacacc aaatatgcgc atcataatga gggcgaacag catggacaac
     1321 ggaatgacag atgccaccac aagaccagcc cgccagttac ccagcaggag taccacaatg
     1381 aaaatgacga tcagtccccc ttctatgaga tttcggctca cagtatgaat ggttcttccc
     1441 acaagttcag agcgatccag ataagggtaa atgtgtactc cttccggcaa ggaggcttcc
     1501 acctcttcga tccgctcctt cacgtttctg atcgcttcag atgagttggc tcctttcagc
     1561 atcagggtta ttccgccaac cacctctccc tgtccatcca tggtcatggc gccatatcgc
     1621 tgtgggctac cgaatccgac tgtagccaca tcgcggatac gaacgggtac accacccttc
     1681 actgttaccg gtatcaagcc aatgtcggcc aggctgccgg ccctgccttc catccggata
     1741 tagaatgcct tgtctgcctg ttccaggtaa cttccaccgc tgtttgcatt gttgtcagca
     1801 agtgccctga aaatatcatc cagggtaacc tcccattgca acatcctaac cggatctgca
     1861 ctgacctcgt actgcctcac ccgtccacca aagctgctca cctcaatgat accttccatt
     1921 cctgcaagct gacgcttgat gacccagtcc tgaatggttc gcagatccat atcagaatac
     1981 tggccggtat gcatggaatc aactgtaagc acatactgat atatttcgcc cagaccagta
     2041 gtgatgggca tcatttcggg cgatcccagt tcagaaggga tctctgcggc agccagctga
     2101 atctgttcac caacgaactg acgtgccttc ataatgtctg tccggtcttc aaatacaatg
     2161 gtgaccactg agagaccata tctggatata cttcttagtt ctgtgacctc cgggatattc
     2221 gccatgtatg attcgatcgg ataggttaca tattgttcca cttcctcggc tgcaagtgtg
     2281 gggctaacgg ttactacctg cacctggttg ttcgtgatgt caggcacagc atctatagga
     2341 atcctggata atgaatcaac tccaactgct gccatggcga ggacagctac cagtacgatc
     2401 agtttattct taacagaaaa tgatattatt ctttcaaaca tgttatattg ttgacaataa
     2461 agcatagcaa ccgatcatag aaccggttgc agaacaaagc agaaattgtc aactaaccgg
     2521 aggaggccgg accgcaaacg gggacccggc gtggctgaat tgcagcggca gatcagtggt
     2581 aaccgacagg catcgaacac atgaaaaaga atggctggca tcaaccgggg cacaaaaaag
     2641 gaatgcacct ggagaagcgt cggcaccctg tgccgaatga agatagttat cggggctgtt
     2701 gagacgtgaa tgattgtgag acaatacatt caaccatgtt atctgcccat gttcgcagat
     2761 atctgcatgc aattctgcta cattattgtc cgcctcattc tgatgcagat gaggcagcaa
     2821 actgtgcatc accaaaatag cggcagcaca ggtcagcagg attgaacgca gtaaagccat
     2881 cggtacaaag gtaatttttt cactactcat ttccgatgac cacaggttac caactgttaa
     2941 ctttaccaca tgtatgtggc ggcagctcca ttggtttata ccctgttgtt cctgatctgg
     3001 ctgcagcgga aaggatattt cggtggcctg catgcacgct ggtggcaggg tgcttttata
     3061 ctacatgtgc tggcaggtat cggcaatctg gcggtctggg tctttgcggt agggcatggc
     3121 gacagcatca actacatcca cgacggacat atgatctggg agacgctgcg ttctcatccc
     3181 tcccattttt tagagctcac cttcggctgg ggtgctgata atgtgtatcc ggaacatttg
     3241 ctttacatct cagaacctct caaatattcc tggaataata ttgaatacac gatggtgcgc
     3301 atccatgcca tcctgttcgt attcactttc ggtaatgcat ggggcaatat cgtcctgctg
     3361 aacatcgcct actttgccgg agccaggttt ctgtatttcc gtttatggca aagacatccg
     3421 gaaagaaaga cagggctgtt cggcctggtg ctgctcattc catcggttgt gatctggagt
     3481 gcgggaaccc tgaaagaagg cccggcactt atgctgatga tgttcatcag cgggctctta
     3541 ctggacatgg aaaaacagtt caggtggcgg tcgctcttct ggctgttcgt ttgtatggcc
     3601 gtcatgctgc tcatccgcga atactatgtc atcttcctgg tacccctgtt gttcttgtgg
     3661 tggatgggac agaaaatttc gcgaagcgga tggttgtttt ttggggcggc cctggcgggg
     3721 atggtagtgg tgttggtggc cgatcgtact tccgatgact actcggttgc agagatcatc
     3781 tgcgaagccc agcaattcta tattccgata gggacagatc cgga
//
LOCUS       DAOMAX010000253         3816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4565_length_3816_cov_4.166977, whole genome shotgun sequence.
ACCESSION   DAOMAX010000253 DAOMAX010000000
VERSION     DAOMAX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4565_length_3816_cov_4.166977"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PK742_11075"
     CDS             complement(<1..415)
                     /locus_tag="PK742_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQU77174.1"
                     /translation="MIRYLGILILTVGMANPSSHTVIPGNDSPPFVPFYERNYHWVDS
                     LMETMSVEEKIGQLFMVAAYSNKDAAHEQAISKLIRDYKIGGLIFMQGGPVRQARLTN
                     TYQSIASIPLLIAMDAEWGPAMRLDSVASFQRQLTW"
     gene            1070..1579
                     /locus_tag="PK742_11080"
     CDS             1070..1579
                     /locus_tag="PK742_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77175.1"
                     /translation="MPRNELQKTTTVTFFWPSLIYSFSFLFSYCDDQQDSLELFIFPP
                     SNCYFVLSLTEGTVYASSITYIYGEDEPYDLVDSSIVSIQGKISALKTDSINLLLDSN
                     INGFSTEDPQLRKYDGFQSKIIIGQRAFFPVGNDVVCMQDIARILSDCIHYEELGCHE
                     YFDLLRLSD"
     gene            1841..2065
                     /locus_tag="PK742_11085"
     CDS             1841..2065
                     /locus_tag="PK742_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77176.1"
                     /translation="MFRTQKPRKFTYVPIYWDPEKEEKEQKQRWRETGGEESSIRFNE
                     EFQSKMNRKTNNTRILIIVILLLILFIFFW"
     gene            2077..>3816
                     /gene="mutL"
                     /locus_tag="PK742_11090"
     CDS             2077..>3816
                     /gene="mutL"
                     /locus_tag="PK742_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQU77177.1"
                     /translation="MSDIIRLLPDNIANQIAAGEVIQRPASVVKELLENAIDAGADRV
                     QVIIKDAGRTLIQVIDNGCGMSVTDARTCFEKHATSKIRNADDLFAIRTMGFRGEALA
                     SIAAIAQVEMKTRLHDEDLGTFIIIEGFEVKSQEPCQTAEGTSIAVRNLFFNVPARRN
                     FLKSNTVELRHIVDEFERVALAHSQVFFSLQHNGVELFHLNKANLRQRIIHLFGNNYN
                     EKLVPIEEQTSVTRLSGFIGKPDSARKTRGEQFFFVNNRFIRSPYLQHAVINAYDQML
                     PDGHYPLYVIFIEIDPARIDVNVHPTKQEIKFEDERIIYTFIQAAARHALAQYSIAPT
                     LDFDQDPTFANLPAFIHPHKEETTVTTGFKVMPSKMNEVEKQAFPDWKKERSSWPKQD
                     EISSKTFPELDDADEGLTVSTAFQVHQRYIISQIRSGYIVIDQQAAHERILFERYLKH
                     LHDKKHHSQQSLFPSTLQVSPGDAALMQDLLPEINQLGFDIQPFGKDTFVIHGVPPEL
                     ENQDERKVLEELIEQCKADQADLKLNKHEILAKSLAKSNAIQAGRKLSNQEMRSLIDE
                     LFACQTPYLSPAGH"
BASE COUNT         1007 a          945 c          980 g          884 t
ORIGIN      
        1 cccaggtgag ttgtctctgg aagctggcca cagagtcgag ccgcatggcg ggaccccact
       61 cggcatccat ggcgatgagc agcggaatgg atgctatgct ctggtaagtg ttggtgaggc
      121 gtgcctgtct gaccggacca ccctgcatga agatgagtcc gccgatctta tagtcacgga
      181 tgagtttgct gatggcctgt tcatgggcgg catctttgtt ggaataggcg gccaccatga
      241 agagctgccc gatcttctcc tcgacggaca tggtctccat gagtgaatct acccagtggt
      301 agttccgttc atagaaaggc acgaagggcg gactgtcatt accgggtatg acggtgtgcg
      361 acgatggatt ggccatgcca acggtcagaa tgagtatgcc caggtatcgg atcatgtgag
      421 taggtgcaga caaaacgctg tgaatgtgaa atagtatgtg ctgctgggtg caaatgtagg
      481 gaggggaacg gggaaggtgg aagggtggaa ggtggaaaag aggaaaaacg gaggtcggaa
      541 ctcgaaattc ggaatgtgag gggatcctga ccacatcttt gaaattgcct ggtgtccttt
      601 gtgcctcctt tgcgtcttcg cgttagcatg cagaacacga tgacacgatg aaggcacgaa
      661 gcgcacgaag gacttcattg aaggatgcgc caaataattg aatgtctttc cgggcaggga
      721 gtctcccttt cagagatgga atctacctca aattacttcg cagaaatcta ctgtcttaaa
      781 aaaccacaga gttcctttca ggtaactctg tggggacaaa aagagaaaag cggaatacga
      841 aatgcggagt tcggaatggg aggggaaggg tttaaggtgg aaaaccggga aaagttcgta
      901 atcggctcaa ggtagcaatc gggattgatg ttaccggtcc gatcggaggt caattcaact
      961 cagctacctg tctctccggg cttgacccgg agtctccttc attggtgaaa gaaacagcaa
     1021 gcccaatcag ctgaacctaa aaagaatctt gttcattggt atatcttaga tgcctcgtaa
     1081 tgaactgcaa aagaccacca cagtgacatt cttttggcca agtctgatat actccttttc
     1141 ttttttgttt agctattgtg atgatcagca agacagcctt gaactattta tttttcctcc
     1201 cagtaactgc tattttgtgc taagcctgac agaaggtact gtgtacgctt caagcatcac
     1261 ctatatatat ggtgaggatg agccgtatga ccttgtggat tccagcattg tatctatcca
     1321 gggtaagatt tctgccctga aaaccgatag tattaacctc ttgctcgatt ctaatattaa
     1381 tgggttctcg accgaagatc cacagttaag gaagtacgat gggtttcagt ctaaaattat
     1441 tattggacag agagccttct ttcctgttgg caatgatgtt gtttgcatgc aggatatcgc
     1501 caggatcttg tctgattgca tccattatga agaattaggt tgtcatgagt attttgatct
     1561 tttgcgtctt tctgattagg caatgcccag atcgatagac catgatcggg cggattttaa
     1621 ggcatattca aacccaactg gagactccgg ctcgggggcc ggagagacag atgattttgg
     1681 tgaatggaca cccttctgag ttattccagc cgtttttcga cacatggtca cccgccggtc
     1741 agaaatcggg ctttgaaggg tgctttccgt atctttggaa gcacgcagca aacgaatcca
     1801 ccaccggctg cgttataccg atatgctggg caaaaattcc atgttccgaa cgcagaagcc
     1861 acgtaagttc acttatgtgc ccatctactg ggatccggag aaggaagaaa aagagcagaa
     1921 gcagcgctgg cgtgaaacag gcggggaaga gtcgtccatc cggttcaatg aggaatttca
     1981 atccaaaatg aaccgcaaaa cgaacaatac ccgcatcctg atcatcgtca ttctgttgct
     2041 gatactcttt attttctttt ggtgaacttt gcggccatgt ccgacatcat ccggctactt
     2101 cctgataaca tagccaacca gatcgccgcc ggcgaggtca ttcaacgacc ggccagcgtg
     2161 gtcaaggagt tgctggagaa cgccattgat gccggggcag accgggtgca ggtcatcatc
     2221 aaggatgccg gacgcaccct catccaggtg atcgacaacg gctgcggcat gagtgtgacc
     2281 gacgcccgca cctgctttga aaaacacgct accagcaaga tccgcaatgc cgacgatctc
     2341 tttgccatcc gcaccatggg attccgtggc gaagcgctgg ccagtatagc tgccatagcc
     2401 caggtggaga tgaagaccag gttacacgat gaagaccttg gaaccttcat catcattgaa
     2461 gggttcgagg tcaagagcca ggaaccctgt cagaccgccg aaggcaccag catcgccgtg
     2521 cgcaatctct tcttcaatgt acccgcccgt cgcaatttcc taaaaagcaa cacagtagag
     2581 ctccgccata tcgtcgatga atttgaacgg gtggcgctgg cacattcgca ggtgttcttc
     2641 agtctgcagc acaacggtgt ggagctgttc cacctcaaca aggcgaacct gcggcagcgc
     2701 atcatccacc tgttcggcaa taactacaat gagaagctgg tgcctatcga ggaacagacc
     2761 tcggtgacca ggctgtcggg attcatcggc aaacccgaca gtgcccgcaa aacaagggga
     2821 gaacagttct tcttcgtcaa caatcgcttc atccgcagtc cctatctgca gcatgccgtc
     2881 atcaatgcct acgaccagat gctgcccgac ggtcattatc cattatacgt cattttcatc
     2941 gagatcgatc ctgcccgcat agatgtgaac gtgcatccca ccaagcagga gatcaagttc
     3001 gaagacgaac gcatcattta tactttcatc caggcagcgg ccagacatgc gctggcacag
     3061 tacagtattg ccccgacgct ggatttcgat caggacccga ccttcgctaa tctgccggca
     3121 ttcatccatc cccacaagga agaaacaacg gtaaccaccg gtttcaaggt gatgcctagc
     3181 aagatgaatg aggtggaaaa acaggcattc cccgactgga aaaaagaaag atcatcctgg
     3241 ccgaaacaag acgaaatttc ttccaaaacc tttcccgaac tggatgatgc cgacgaaggc
     3301 ctgactgtga gtaccgcctt tcaggtacac cagcggtata tcatttcaca gatcagatca
     3361 gggtatatcg ttatcgacca gcaggccgct catgagcgca ttttgttcga acgttacctg
     3421 aagcacctcc acgataaaaa acaccacagc cagcagtcgc tgtttcccag taccctgcag
     3481 gtatcacccg gagatgcagc cctgatgcag gacctccttc cggagatcaa ccagctgggt
     3541 tttgacattc agccctttgg caaagacacc tttgtgatcc atggtgtgcc gcctgaactg
     3601 gagaaccagg acgaacgaaa agtgctggaa gaactgatcg aacaatgcaa ggcagaccag
     3661 gccgatctga agctcaacaa acatgagatc ctggccaaaa gtctggcaaa gagcaatgcc
     3721 attcaggcgg gcaggaaact cagcaaccag gaaatgcgtt cattgataga tgaacttttt
     3781 gcctgccaga cgccttattt atcaccggcc ggtcac
//
LOCUS       DAOMAX010000254         3802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4590_length_3802_cov_4.706699, whole genome shotgun sequence.
ACCESSION   DAOMAX010000254 DAOMAX010000000
VERSION     DAOMAX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4590_length_3802_cov_4.706699"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="recO"
                     /locus_tag="PK742_11095"
     CDS             complement(<1..423)
                     /gene="recO"
                     /locus_tag="PK742_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQU77178.1"
                     /translation="MLHHTKGIVLRTTPYSESSVVVLVYTEKFGRQTYIINGVRSARS
                     KGKSALYQHGNLLDMVVYHKETGDIFRVSEASIAHAYTLAPFDIVRSSIMLFLVELMT
                     RAISETEANEELFRFCWQAFLQLDRPGGQLSLYPHRYML"
     gene            complement(497..2851)
                     /locus_tag="PK742_11100"
     CDS             complement(497..2851)
                     /locus_tag="PK742_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77179.1"
                     /translation="MLLKRTFFFLFFLVAATLLKAQTQEPPVAIGSWKAYLPYGGPDV
                     IALAGQKVYAATGLSVFNLNLQDNSLERLHKANGLDDINAIGVYYGKETGTVAISYLN
                     SNMDFIIDGELVNFPYIRTAGISGNKSINQVQFSGDTAFIATGFGIVVYQISEKESPA
                     TWFFTDADGAYINVRSVLTQDDQIFAATDVGLYVAGKDEPLLEDFSRWTLLSGTTLPT
                     GSAGWLTPWNDQVTVTINDEDIYLFDGTDWSLYYAEADWQVRTCRSAGNGLQVLLIQG
                     DEEIPEDYRMVDIADDGSVSELNTEGRFTAPNDFIRDADGAYWVADGFGLLTEISNDE
                     YRSYLPNGPGSSSVAEMEYYDGRLWVAPGSINASWNYTYNRDGFFVLDYGFWNNYNQY
                     DLPALTDVFDFITMTLDAATGIAWLGSFGSGVFSFDKATGMLTRYDQTNTGDMGLQDL
                     GAADPGSCRIGGMIFDTEGNLWVSNYAASNPLVVRKADGSWMHFDCNLPLETGEQVGQ
                     IILDDFNQKWVQLPRGNGILVFDHGADIDNTADDMSRVLGVGAGNGNLPVAYVNCLVK
                     DLDGEIWAGTNEGVSIFYNPASVMDGGTIADAAQPLVNLGGYNEYLLSKEIVNCITVD
                     GANRKWIGTNSGVFLISADGTEQLLFFNQDNSPLLSNVVLSIAIDGVTGEVYFGTDKG
                     IVSYRGTATQGLAEQGNVKVFPNPVRPEHNGPIAISGLVNNAQVKITDTGGRLIYETI
                     ALGGQAIWNGKGYNGQDAATGVYMVYSADDTGKETVVTRIVLLR"
     gene            2986..>3802
                     /locus_tag="PK742_11105"
     CDS             2986..>3802
                     /locus_tag="PK742_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQU77180.1"
                     /translation="MWQSISRIILRNRVIFLAGWILLTIFMGLETTHVKLSYDLNKSI
                     PANAQVNLDYEAFKNEFGDEGNLVVIAIRSDDFFQLDFFRQWDSLSQELTTIPDVQNV
                     LSVPQTMRMVRNAPERKFDIAPVFNHLPDTQAELDSLKEEFYKLPFYDRRLYNEDRDV
                     FLMLVSLNKDILSTVRRITVMDDIYTFTRPYEERTGNHLYYTGLPVIRHYQLTTISGE
                     ILLFLLLAVIVTIVVLFILFRSWIAVVFPLLFIGSGVVFSMGLMSLIGFKVTLL"
BASE COUNT          928 a          963 c          897 g         1014 t
ORIGIN      
        1 cagcatatac ctgtgcggat acaacgacaa ctgacctccg ggccggtcca gctgcagaaa
       61 agcctgccag cagaatctga atagctcttc attagcttct gtttcagaaa tggccctggt
      121 catcagttcc accagaaaca acatgatgga gctgcgaacg atgtcaaatg gtgcaagcgt
      181 ataggcgtgt gcaatggatg cttcactgac ccggaatata tcgccggtct ctttgtgata
      241 taccaccata tccagcagat taccatgctg gtataaggca ctcttacctt tggaacgggc
      301 tgatctgact ccgttgatga tatatgtttg ccgcccgaat ttttctgtat aaacaagaac
      361 aaccacgctg ctttcactgt agggtgtggt tcgcagtaca atgcctttgg tatggtgcag
      421 catgatacaa acttactgga aatcagttcg gaatgcgctg actaaaagag gtgaaaaaca
      481 ggaacaggtt ctgtacttag cgcaacagta cgatcctggt cacaacggtt tctttcccgg
      541 tatcatcggc actgtaaacc atatagacac cggtagcggc atcctgacca ttatatccct
      601 ttccgttcca gatggcctgc cctcctaatg caatagtttc atatatgagt cttccgcctg
      661 tatcagtaat cttgacctgg gcgttattga ccagtccgct gatggcaatt ggcccgttgt
      721 gttcgggcct gacaggattg ggaaaaacct tgacattacc ctgttcagca agtccctgcg
      781 tagcagtacc cctgtaggat acgatgcctt tgtcggtgcc gaaatatact tcgccggtca
      841 ctccatctat ggcgatactc agtactacat tgcttaacag cgggctgtta tcctgattaa
      901 aaaacaacag ctgttctgtg ccgtctgctg aaatgagaaa cacaccgctg ttggtgccta
      961 tccattttct gttggcgcca tccaccgtga tgcagttgac gatctcttta ctgagcagat
     1021 attcgttgta accacccaga ttgaccagag gttgtgcagc atctgcaatg gtaccgccat
     1081 ccatcacaga agcaggatta tagaaaatgg atactccttc attggtacct gcccagatct
     1141 ctccgtccag atctttgacc aggcagttca cataggcaac aggcaggttt ccgttacctg
     1201 cgccaacacc gagcacacgt gacatatcat ctgcggtatt atctatgtca gcaccgtggt
     1261 caaacaccag aataccatta cctcttggca actgtaccca tttctggttg aagtcatcca
     1321 gtatgatctg tcctacctgt tcaccagttt ccaggggcag gttacaatca aagtgcatcc
     1381 aggatccatc ggctttacgc actaccagcg ggttgctggc agcatagttg gaaacccata
     1441 ggttgccttc tgtgtcaaag atcattccac caatacggca gctgccggga tctgctgctc
     1501 ccaggtcctg caaacccata tcgccggtat tggtctgatc ataccgggtc agcatacctg
     1561 tagctttatc aaaggagaac acaccactgc caaatgaacc cagccaggca ataccagtgg
     1621 ctgcatccaa ggtcatggtg ataaaatcaa acacatccgt cagtgccggc aggtcatact
     1681 gattataatt attccagaat ccatagtcca gcacaaagaa gccgtcgcgg ttgtatgtat
     1741 agttccagga agcattgatg gagcccggag ccacccatag ccggccgtcg taatattcca
     1801 tttcagccac actggaagat ccgggtccgt ttggcaggta gctccggtat tcatcgttgc
     1861 tgatctctgt aagcagcccg aaaccatcgg ctacccagta agctccatcg gcatctctaa
     1921 taaaatcatt gggagctgtg aaccgaccct cagtgttaag ctcagaaacg gatccgtcgt
     1981 cagcaatatc taccatgcgg taatcttccg gaatctcctc atcgccctga atcagcagga
     2041 cctgtaatcc gtttccggca gatctgcatg tgcgcacctg ccagtctgct tctgcatagt
     2101 acaaagacca gtcggtgccg tcaaacagat aaatatcttc gtcattgatg gttaccgtta
     2161 cctgatcatt ccagggggtc agccagccgg cagaaccggt aggaagagtc gtgccactca
     2221 ggagggtcca cctggaaaag tcttccagca gcggttcatc tttgcccgcc acgtataacc
     2281 ccacatcggt tgctgcaaat atctgatcat cctgtgtaag tactgaccgc acgttgatat
     2341 aagcaccatc ggcgtctgta aagaaccatg ttgcagggct ttctttttct gagatctggt
     2401 acaccacgat gccgaaacca gtagcgatga aagcagtatc gccggaaaac tgcacctgat
     2461 tgatggattt gttgccgctg atgcctgctg ttctgatgta ggggaaattg accagttcgc
     2521 catcaatgat gaagtccatg ttgctgttca gatagctgat ggctactgta cccgtctcct
     2581 ttccgtaata aaccccgatg gcgttgatat catccagtcc gttggccttg tgcagtcgtt
     2641 ccagactgtt gtcctggagg ttgaggttaa agacactgag accggttgca gcatagacct
     2701 tttgaccggc cagtgcgatc acatcagggc ctccgtaagg cagataggct ttccaggatc
     2761 cgatggctac cggaggctcc tgtgtctgtg ctttcagcaa tgtggcagct accaggaaaa
     2821 agagaaagaa gaacgtacgc ttcagcaaca tgtggtacaa agttcagaaa tcggcaggat
     2881 aacgggtttt ttcagggcat attttgcagt tttttgtcag ctttcctgtc tttgaacgaa
     2941 ggctcaggcc cttacttttc gtatctttgc acctattttt cagccatgtg gcagtctatc
     3001 tccaggatca ttttgcgcaa ccgggtcatt tttctggccg gatggatcct gcttaccatt
     3061 ttcatggggc tggaaaccac ccatgtcaag ctgagttatg acctgaacaa gtctatacca
     3121 gccaatgctc aggtaaatct ggattacgag gcattcaaaa atgaatttgg cgatgaaggc
     3181 aacctggtgg tgattgccat ccggtctgat gatttttttc agcttgattt tttcaggcaa
     3241 tgggatagtc tcagccagga actgaccact atacctgatg tgcagaatgt tttatctgtt
     3301 ccgcaaacca tgcgcatggt gcgcaatgct ccggagcgga agtttgatat tgctccggtg
     3361 ttcaaccacc tgcctgacac acaggctgaa ctggattcgc tcaaggaaga attttataag
     3421 cttccgtttt atgatcgccg gctgtacaat gaagaccgcg atgtttttct catgctggtc
     3481 agcctgaaca aggatattct cagtacggtg cggcgcatca cggtcatgga tgatatctac
     3541 actttcacca gaccatatga agagcgaacc ggaaaccatc tgtattacac gggcttgccg
     3601 gttatcaggc actaccagct gaccaccatt tcaggtgaga tcctgttgtt tttgctgctg
     3661 gcagtcattg ttacgatcgt ggtgttgttc attttgttcc ggtcatggat agcggtcgtg
     3721 ttccctttac tcttcatagg aagcggtgta gtgttcagca tggggcttat gtcacttata
     3781 ggattcaagg tgaccttgct ta
//
LOCUS       DAOMAX010000255         3797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4603_length_3797_cov_3.768306, whole genome shotgun sequence.
ACCESSION   DAOMAX010000255 DAOMAX010000000
VERSION     DAOMAX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_3797_cov_3.768306"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PK742_11110"
     CDS             <1..192
                     /locus_tag="PK742_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77181.1"
                     /translation="KLDSHLIINDMAASPKEIIVSGVPLQVGQARYLGNLLHENKPDP
                     ENYSQTAIVLADENILFPV"
     gene            328..1020
                     /locus_tag="PK742_11115"
     CDS             328..1020
                     /locus_tag="PK742_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77182.1"
                     /translation="MRFHANEPDTLKEIYIHWAYVDGPKSNLFFSLMVWDVIDTTGAS
                     ADESILYQADFLTPKYVDSVNGFYVYQLTDYLGNPTPVVVNGYFYVGWLQTQSDFLNV
                     GFDVNNVANNDVFYTTGGNWTRSDIPGAIMIRPQVGGNYSVVDDVHDVQTTSVIVFPN
                     PATAQISMSGMTEGDRYEIFSSTGVLVDAGIWTGDPVEITDLASGMYLLRAIHRDGDS
                     VTTSFIKQHTWK"
     gene            1032..1316
                     /locus_tag="PK742_11120"
     CDS             1032..1316
                     /locus_tag="PK742_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQU77183.1"
                     /translation="MKSRLDAGEDLLLIDVREPYELEEFSLDAVNIPLATIPVRMYDF
                     DDDLDREIVVFCRSGVRSAAAQAMLKQAGFTSVLNLSGGVLAWMEQLGSR"
     gene            1313..1738
                     /locus_tag="PK742_11125"
     CDS             1313..1738
                     /locus_tag="PK742_11125"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HQU77184.1"
                     /translation="MSRPSADPEFPVLFFDGYCHLCDRSVQFVLRFDRKKRIRFAALQ
                     SDYATRFFTKQSFHPVNDSLILWYKGKFFEQSDAALRIAGLMGFPVHLLVVLYVFPGS
                     LRNVVYRWIAKNRFKWLGKRDACRMPEPGERNRFLDQTL"
     gene            1748..3652
                     /locus_tag="PK742_11130"
     CDS             1748..3652
                     /locus_tag="PK742_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU77185.1"
                     /translation="MRKWIVAVCLCFLVLLFSYCRQQAGSAVSSDSPYLNHSDTAEYV
                     GMSVCRQCHADKFSTFVHTGMGMSFGLATKEKSAALFGPHVQVYDSILDFYYHPFWQG
                     DSLMIMEFRLDGADTMHKRTEHISYIIGSGQHTNSHLLQVNGYLYQAPITFYTQKQEW
                     DMAPGMSGGFNSRFGRVIESECVTCHNGLPEQVAGSVNKYTGIPLGIDCERCHGPGSI
                     HVARISSGIAVDTANAFDYSIVNPRGLSTELQNDLCFRCHLQGINVLHPGKEFADFKP
                     GQHIRDYWNIFLPRFDGKNDQFLMASQADRLVQSKCFIESGKISCITCHNPHVTVKET
                     PISLFNKPCLDCHQLPQSTCTETLEVRSLQNDNCSGCHIPKSGSIDIPHVSISDHKIQ
                     IPGREQEKEDGKFTGLVALTDPDIEPVEMARGYLRYYEGFVSESAMLDSAKAWLERSR
                     HDDLYDATQAHLWYLQGNMQGLLQMDVKMGNKVPDSWTAYRLGEALLQEGNYSAAANR
                     FKQAVDQLPLQLDFRFKYASALYLDGQTEDAGKEFLFITRENPGYEKAWMNLAVWYIN
                     KGDVVAAEEALQNAVALNPDYLQARLTLTDLYLRTRQRTKAGVMMQYLLRYHRDEPLV
                     QELNMRYRSL"
BASE COUNT          969 a          843 c          970 g         1015 t
ORIGIN      
        1 aagctggaca gtcatttgat tatcaatgat atggccgctt cacctaaaga aatcattgtt
       61 tccggtgttc cgcttcaggt aggacaagcc aggtatttag ggaatctttt gcatgagaat
      121 aaacccgatc cagagaatta cagccagacc gccattgtcc tggcagatga aaatattctt
      181 tttcccgtgt agtactccct tcccggtgat attgaaaggc tgaatgatac cctgcgcata
      241 cagcaggatt tttataacta ctttgcctac gatgatggct ctgccgaaaa agcctatgca
      301 ctgaatggta caggaagtaa actcgcagtg cgatttcatg ccaatgaacc tgatacctta
      361 aaagagatct atattcactg ggcttatgtg gatggaccaa agtccaacct gttcttttct
      421 ctgatggtct gggatgtcat tgacacaacc ggtgcttcgg ccgatgaatc catcctgtat
      481 caggcagatt ttctcacgcc aaagtatgtt gacagtgtca atgggttcta tgtgtaccag
      541 ctgaccgatt acctcggcaa tccaacaccg gttgtagtga atggctattt ctatgtgggc
      601 tggctgcaaa cacaatcaga ctttctgaat gttggctttg atgtgaacaa tgttgccaac
      661 aatgacgtgt tctatactac tggaggaaac tggaccaggt cagacattcc gggcgccatt
      721 atgatcagac cacaggtggg aggcaattat tcagtagtgg atgatgtgca tgatgtgcag
      781 accacctccg tgatagtttt tccaaatcct gccactgcac agatcagcat gagcggcatg
      841 accgaaggcg acaggtatga gatattcagc agcacaggag tgttggtaga tgccggtatc
      901 tggactggtg atcctgttga aataactgac cttgccagtg gcatgtacct tttgagggcc
      961 attcatcgtg atggggatag cgtaacaaca tccttcataa agcaacatac atggaaatag
     1021 atgtacttac tttgaaaagt aggttggatg caggcgaaga tcttctgctg atcgatgtcc
     1081 gggagcccta tgaactggaa gagttctctt tagatgctgt caatattcct ttagccacca
     1141 ttcctgtgcg catgtacgat tttgatgatg acctggacag ggagatcgtc gtgttctgca
     1201 gatcaggcgt gcgcagtgcc gcagctcagg ccatgctgaa acaggccgga tttaccagtg
     1261 tgcttaacct gagtggaggg gttctggcgt ggatggaaca actgggcagc aggtgagcag
     1321 accatcggca gatcctgaat ttcccgtttt gttttttgat ggctattgtc atttgtgcga
     1381 tcgttccgta caattcgttt tgcgattcga tcgtaaaaaa aggatccgtt ttgcggcttt
     1441 gcaatctgac tatgctacac gtttttttac caaacagtcg tttcatcctg tcaatgactc
     1501 tctgatactc tggtacaaag ggaagttctt tgaacagtct gatgctgccc tgcgcattgc
     1561 aggtctgatg gggtttcctg ttcatctgct cgtagttctg tatgtattcc ccggaagtct
     1621 tcgcaatgtc gtctatcgct ggatagcaaa gaatcgtttc aaatggctgg gcaaaagaga
     1681 tgcctgcaga atgccggaac ccggagagcg caacaggttt ttagatcaga ccttataact
     1741 ttggttcgtg cgaaaatgga tcgttgcggt ttgtctgtgc tttctggtac tcctgttcag
     1801 ttattgcagg caacaggcag gcagtgccgt ctcgtctgat tcaccttatc tcaatcacag
     1861 tgacactgca gagtatgttg gcatgtcggt ttgcaggcag tgtcatgcag ataagttcag
     1921 cacctttgta cataccggaa tgggcatgtc atttggcctt gccacaaaag agaaaagtgc
     1981 tgctttgttc gggccgcatg tgcaggtcta tgattccatt cttgattttt attaccatcc
     2041 tttctggcag ggagattccc tcatgatcat ggagttcagg ctggacggag ctgacaccat
     2101 gcataaaagg acagagcaca tcagctatat catcggatcc ggtcagcata ccaactcgca
     2161 cctgctacag gtgaacggct atctctacca ggcacccatc actttctata cccagaaaca
     2221 ggagtgggat atggcacccg gaatgagtgg tggattcaac agtcgctttg gtcgggtcat
     2281 tgagtcagaa tgtgtgacct gtcataacgg actgccggaa caggtggcag gttctgttaa
     2341 taaatacaca ggcattccgc ttggcataga ctgtgaacgc tgtcatggcc ccggcagcat
     2401 tcatgtagcc aggatatctt ccggcatagc tgtagatacg gccaatgcgt ttgattacag
     2461 catagtcaat cctcgggggt tatcgaccga actacagaat gacctctgct tccgctgcca
     2521 tctgcagggc atcaatgtat tgcatccggg taaggaattt gccgacttca aaccgggtca
     2581 gcatatccgc gactactgga atattttcct gcctcgtttt gacgggaaga atgatcagtt
     2641 cctgatggca tcgcaggcag acaggctggt gcagagcaaa tgctttatcg agtcgggaaa
     2701 gatatcttgc attacctgtc ataatccgca tgtcacggtg aaagagactc ccatatcatt
     2761 gttcaacaag ccttgtttgg attgccatca gttaccgcaa agtacttgca cagaaacact
     2821 ggaggtgagg tctttgcaaa acgataattg ctcagggtgt catataccga agtcgggcag
     2881 cattgatatt ccacatgtgt ctatctctga tcataagatt cagataccag gtagagaaca
     2941 ggaaaaggag gatggaaaat ttaccggcct tgtagcactt acagatcctg atatcgagcc
     3001 cgtagaaatg gcaagaggat acctgcggta ctacgaagga tttgtaagtg aatcagccat
     3061 gctggattct gccaaagcat ggttggaaag aagcaggcat gatgatctgt atgatgccac
     3121 acaggcgcat ctgtggtact tgcagggcaa tatgcagggg ctgctgcaga tggatgtaaa
     3181 aatgggaaat aaagtacctg attcctggac ggcctacagg ctgggcgaag ctttattgca
     3241 ggagggaaac tactctgccg cggcaaacag gttcaaacag gctgttgatc agctgccatt
     3301 gcaactcgat tttcggttca agtatgcttc tgcattgtat ctggacggac agacagaaga
     3361 tgccggcaag gagttcctgt ttattacccg ggagaatccg ggatatgaga aggcctggat
     3421 gaatctggca gtctggtata tcaataaagg ggatgtggtg gctgccgaag aagctttaca
     3481 gaatgcggtt gcacttaatc cggattacct gcaggccaga ctgactctga ccgatctgta
     3541 cctgcgcacc agacaaagaa ccaaagccgg agtcatgatg caatacctgc tgcgatatca
     3601 ccgtgatgaa ccacttgtac aggaactgaa tatgcgctac cgatctttgt aatacatttg
     3661 ttgcattatc aagggcttgc gatcgatcat acaacagata acagacctgt acgaccagct
     3721 ggtcaggcgt atgcgtatct tttttcagcg caccagcctg ccggggtttt cgggtatccc
     3781 gctgtatgat gtatgga
//
LOCUS       DAOMAX010000256         3755 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4683_length_3755_cov_4.257838, whole genome shotgun sequence.
ACCESSION   DAOMAX010000256 DAOMAX010000000
VERSION     DAOMAX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_3755_cov_4.257838"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1021
                     /locus_tag="PK742_11135"
     CDS             176..1021
                     /locus_tag="PK742_11135"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HQU77186.1"
                     /translation="MSDVQHTCVSCGQTHEGLYCHVCGEKRLDPNDRKLKYYLHQLVS
                     AVTFADNKAWRTLRSVIVNPGKLSLDYAAGRRVRYTAPVSLFFIGNLIYFLIPILETF
                     DSSLYVQTQMMPYKSWIMPSVEGRLADRSISMDEYAVAYNAASTSNAKLLLITMVFLI
                     SIGFAVVAMKKNRYFGDHFIMSLEVNIYNLFVNTIALALLIFPVVLIGQLAGADLRPY
                     LNDQLFTVLILFSLIYFLFRSFRTMYACTTGGAAWRTVFMLAWVAVSLQAYRFILFWV
                     TFYTT"
     gene            complement(1065..2471)
                     /locus_tag="PK742_11140"
     CDS             complement(1065..2471)
                     /locus_tag="PK742_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HQU77187.1"
                     /translation="MENMDLLKISYLLGSLSFIVGLKMLSSPDSARKGNLYAAVGMLI
                     AILATIFFHEHTNAETGEVERIGNLGYIIGGIAIGTIVGYLAAMKVKMTAMPQMVSLF
                     NGMGGACAALIGIVEMSTHHPEFWGERLIIFLGLMIGSVSFSGSMIAYGKLEGKIRDI
                     GSKVQQVFNNTFVFAILALVIYASIQPDVLSGNAIWVIFGCSLLYGILFVMPIGGADM
                     PVVISLLNSFTGVAAAFGGFLYDNQVMLTGGILVGSAGTILTILMCKAMNRSLWNVII
                     GAFSASGGPAKSTEGLSIREINVTDSAILCSYAQRVVIVPGYGLAVAQAQHVCHEFEK
                     VLEDKGVEVEFAIHPVAGRMPGHMNVLLAEADVPYPKLKEMDDINPSLPNTDVVIVIG
                     ANDVVNPAAETDPSSPIYGMPIIKANTAKNIIVMKRSMAKGYAGIENELFYDDKTRML
                     FGDAKATLEKLIAEVKSV"
     gene            complement(2498..2827)
                     /locus_tag="PK742_11145"
     CDS             complement(2498..2827)
                     /locus_tag="PK742_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQU77188.1"
                     /translation="MTILSNTITIFGIDTPWDVIYVIIIAAFLGVEVISNVPAILHTP
                     LMSGANAIHGVVIVGSIIVMGHADPGNTLALVLGFLAVILGTLNVVGGFVVTNRMLEM
                     FRKKEKK"
     gene            complement(2838..>3755)
                     /locus_tag="PK742_11150"
     CDS             complement(2838..>3755)
                     /locus_tag="PK742_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HQU77189.1"
                     /translation="IQPIGRQELDSLKGKVLVGGFNPLVNTDFVKDALGAGVSAFSLD
                     VIPRTTRAQAMDILSSMATVSGYKAVLVAASHLPRFFPMFMTAAGTITPAKVLVLGAG
                     VAGLQAIATAKRLGAVVEASDVRQAAREEVLSLGAKFVDVEGATEDKSAGGYAVEQTE
                     EFKQRQQQAVQEHAMKSDVVICTAQIPGRKAPILLLKDTVAHMKPGSVIIDLAASTGG
                     NCEVTEDGKTIQVNGVTVVGDSGLAATMPYDASKMFGKNIINFLKIMIDKDGAIQLNW
                     EDDLITGTCIAHAGEIRNERVKNIMQVSA"
BASE COUNT          939 a          957 c          859 g         1000 t
ORIGIN      
        1 gttgtgtttg aacaggtttg cgatggtagc gaagaagaaa tactggttga aaccggactt
       61 ccttcaggca tgtatctggt ccagcttaga tcagcacaga gctgtcagga gatcaggctc
      121 atcattcaat agcgaagtat ttaggcgtta agaattgacc ggatcatttc ctggcatgtc
      181 tgatgtacaa catacctgcg tatcctgtgg ccagacacat gaaggtttat actgtcatgt
      241 gtgtggtgag aaaagactgg atccaaatga ccggaaactg aaatactacc tgcaccagct
      301 ggtcagtgca gttacttttg ctgataataa ggcttggaga accttgcgtt ctgttattgt
      361 aaaccccggt aaactcagcc ttgattatgc agctggcaga agggtcaggt acactgctcc
      421 cgtatcctta ttctttattg gtaacctgat ctatttcctg attcccattc tggaaacatt
      481 tgattccagc ctgtatgtac agacacaaat gatgccttat aaaagttgga tcatgccgtc
      541 tgtggaaggc agacttgcag atcgcagtat ctctatggat gaatatgcag tggcctataa
      601 tgcagcatcc acctccaatg ccaaactgtt attgatcacc atggtgtttc tgatcagcat
      661 tggttttgca gttgttgcga tgaaaaaaaa ccgatacttc ggcgatcatt ttatcatgtc
      721 actggaggtc aatatatata atctttttgt aaatactatt gcactcgcct tgctgatctt
      781 tccggtagta ctcattggtc agctggcagg agcagacctc aggccatatc ttaatgatca
      841 gctgttcact gttctcatcc tcttttccct gatctatttt ttattcagaa gcttcagaac
      901 aatgtatgca tgcaccacag gaggtgcagc ctggagaaca gtgttcatgc ttgcatgggt
      961 agctgtgagt ctgcaggctt atcgttttat tttgttttgg gtcactttct ataccacata
     1021 aaaaaagccg gtcactgacc ggccttcttt tatggttgtt cttatcaaac gcttttcact
     1081 tcagcgatca gtttttccag ggttgccttg gcatcgccaa acagcatccg ggtcttgtca
     1141 tcatagaata attcattctc gataccggca tatccttttg ccatgctgcg cttcatcaca
     1201 atgatgttct tggctgtatt agccttgatg atgggcatac cgtaaatagg agaggaagga
     1261 tccgtttctg cagccgggtt gaccacatcg ttcgccccga tcacaatgac cacatctgta
     1321 ttgggcagtg acggattgat gtcatccatc tccttcagtt tcggataggg aacatctgcc
     1381 tcagcgagca atacgttcat gtgtccgggc atccgacctg ctaccggatg gatagcgaat
     1441 tcgacttcca cacccttatc ctccagcact ttttcgaatt catgacatac gtgctgagcc
     1501 tgggcaactg ccagtccata tccgggtacg atcacaaccc tttgtgcata gctgcacagg
     1561 atagcggaat ccgttacatt gatctcacgg atggacagcc cttcggttga tttggccggt
     1621 ccgccgctgg cactgaaagc acctatgatc acattccaca gtgagcggtt catggcctta
     1681 cacatcagga tggtcaggat ggtaccggca gaacctacca ggataccacc ggtcagcatc
     1741 acctggttgt catacagaaa tccgccaaag gcggcggcta ctccggtaaa tgaattcagc
     1801 aatgatataa caaccggcat gtcagctcca ccaataggca tcacaaacag gataccatac
     1861 agcagggaac atccgaagat gacccagatg gcatttccac tcaatacatc aggctgaatg
     1921 gaggcatata ttaccaaagc aagaatggca aaaacaaaag tgttgttaaa tacctgctgc
     1981 accttgctgc caatgtcgcg gatcttgcct tccagcttgc cgtaagcgat catcgaaccg
     2041 ctgaacgata ctgaaccgat catcagtccg aggaagataa tgagacgctc accccagaat
     2101 tccggatggt gggtgctcat ttccacgatg ccgatcaggg ccgcacaggc gccacccata
     2161 ccattgaaca aggagaccat ctgcggcata gccgtcatct tgaccttcat ggctgccaga
     2221 taacctacaa tggttccgat ggcaatacca ccaatgatat atccgagatt accgatacgc
     2281 tcaacctctc cggtttcggc atttgtatgt tcatgaaaaa agatggtagc cagaatggcg
     2341 atcagcatac caactgcagc atacagattc ccctttcggg cagaatcggg actcgacaac
     2401 atttttaatc ccacaatgaa actcaaagat ccaagcagat agctgatctt caacaggtcc
     2461 atgttctcca taacttatgt attgggctga gagccggtta tttcttttct ttttttctga
     2521 acatttccag catgcggttg gtcaccacga aaccacctac cacgttgagt gttccgagga
     2581 tcacggccag aaaacccagc acaagtgcca gggtatttcc aggatctgca tgtcccataa
     2641 ctatgatgga accaactatc accactccgt gaatagcgtt ggcaccactc atcaaaggtg
     2701 tgtgcaggat ggccggtaca ttgctgatca cttccacccc taaaaaggcg gcaatgatga
     2761 tcacatagat cacatcccag ggagtatcta tcccgaagat ggtgatggta ttacttaaga
     2821 tggtcatagg taatttttta agcggatact tgcatgatgt tcttcacccg ttcattcctg
     2881 atctctccgg catgggcaat acaggtgccg gtaatgagat cgtcttccca gttcagctgg
     2941 atggctccat ccttatcgat catgatcttc aggaagttga tgatattctt gccgaacatc
     3001 ttggaggcat catatggcat ggtagcagcc aggccgctgt cgccaactac cgtcacaccg
     3061 ttcacctgaa tggtcttgcc gtcttcggtc acttcgcagt ttccgccggt agatgctgca
     3121 aggtcaatga tcacagagcc gggtttcata tgtgctacgg tatccttcaa cagtagtatc
     3181 ggagcctttc ttccgggtat ctgcgctgta cagatgacca catcactttt catggcgtgt
     3241 tcctgtacag cctgctgctg ccgctgcttg aattcttcgg tttgttctac ggcatatccg
     3301 ccggcacttt tgtcttcagt ggcgccttcc acatccacga acttggcacc cagagagagc
     3361 acttcttcgc gggctgcctg acgcacatca cttgcttcta caacagcacc cagtctttta
     3421 gcggttgcaa tggcctgaag accagccaca cctgcaccca gtaccaatac cttggcaggt
     3481 gtgatggtac ctgctgcggt catgaacatg gggaagaagc ggggaaggtg tgaagcagca
     3541 accagcactg ccttatatcc ggacacagtg gccatactgg acaggatatc catggcctgt
     3601 gccctggtgg tacgcgggat gacatccagg ctgaatgcag atacgcctgc gcccagtgca
     3661 tcctttacaa aatcagtatt gaccaggggg ttgaaaccac ccaccagtac ctttcctttc
     3721 aaggaatcca gttcctgcct tccgatcggc tggat
//
LOCUS       DAOMAX010000257         3743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4709_length_3743_cov_3.399132, whole genome shotgun sequence.
ACCESSION   DAOMAX010000257 DAOMAX010000000
VERSION     DAOMAX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4709_length_3743_cov_3.399132"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..648)
                     /locus_tag="PK742_11155"
     CDS             complement(103..648)
                     /locus_tag="PK742_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQU77190.1"
                     /translation="MQILVKGSCLLLLSCLCSLSDAQSAYVEEGTASYYHDKFVGRKT
                     ASGEIFTQDKMTAAHKSLPLGTWVMVTNKANDSVVILRINDRMPQWNKRSIDLTYSAA
                     ERLGYIRAGLAKVRIEVIDPPNETPPPPDPGPPATVFIEPLPVKAIPVSNKLIEPRYP
                     LNYADLLPKEEKKVWWKVWPR"
     gene            complement(692..1174)
                     /locus_tag="PK742_11160"
     CDS             complement(692..1174)
                     /locus_tag="PK742_11160"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQU77191.1"
                     /translation="MFSSCVPSPAGYFVAESDGKSLSRCYFSDTAPVGVSDPFTEQAA
                     HWFRLYLTDPGTPLPHFELPAASDFSKLVWRALLRIPPGGSTTYLSLARQLGGNQYSR
                     AVGAAVGANPLLVLIPCHRVIGSDGSLTGYAGGIHRKEWLLAHEGLISGVQPSLFPGS
                     "
     gene            complement(1178..2413)
                     /gene="clpX"
                     /locus_tag="PK742_11165"
     CDS             complement(1178..2413)
                     /gene="clpX"
                     /locus_tag="PK742_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQU77192.1"
                     /translation="MKKNDDQHCSFCGRPKTEVNILIAGQEGHICENCVGQAQQLIEE
                     EMQHTGKRYKFGMNAIMKPKAIKQFLDQYVIGQEEAKKILSVAVYNHYKRLNQPKIEE
                     VEIEKSNIVLVGETGTGKTLLARSIARCLNVPFTIVDATVFTEAGYVGEDVESILSRL
                     LQVCNYDVAAAERGIVYIDEIDKISRKTDNPSITRDVSGEGVQQALLKMLEGTEVYVP
                     PQGGRKHPEQKMVKINTQNILFICGGAFDGIQKIIARRTSTQLIGFKKEEEAVVDKEN
                     LLQYVNPQDLKQFGLIPELIGRLPVLTHLDPLDAETLRAILSEPKNALIKQYKQLFQI
                     EGIRLEIDDEVLDFIVDKAMEFKLGARGLRSICEALLTDAMFDMPSAENKDVEFRVDL
                     EYAESKLNQSRINKLKVAI"
     gene            complement(2413..3105)
                     /gene="clpP"
                     /locus_tag="PK742_11170"
     CDS             complement(2413..3105)
                     /gene="clpP"
                     /locus_tag="PK742_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQU77193.1"
                     /translation="MPNNEFKKFATRHMGIPTTTLESYMQSTSQFVTNFTPYIIEERQ
                     LNAQALDVFSRLMMDRIIFLGTPIDDYVANVIQAQLLFLDSVDSKRDVQIYINSPGGI
                     VYSGLAIYDTMQYINPDVATICTGIAASMAAVLLCAGHAGKRSALTHSRVMIHQPLGG
                     MQGQASDMEIALKETLKLKAELYNIISQHSGQGFNKVEEDSDRDYWMTAAEAKDYGMV
                     DEVLSRNPRKTA"
     gene            complement(3095..>3743)
                     /locus_tag="PK742_11175"
     CDS             complement(3095..>3743)
                     /locus_tag="PK742_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77194.1"
                     /translation="EPSEEIVEAIQPGFECKVDRIVRLIPAELNEAFFQSVSAEYGEV
                     TDESALRERISGFIEQYNDNMTRVQLDNTLYKYLIEQTSIDLPDQFLEKWFINSRAAD
                     GEDEETFSQFMRRLKESLIFRKVQQEHQLEATQDEIIQAAVQHVRNSYGQLGEDFVRY
                     IVESQLKERSFVESMHDRVMQEKFLDVIRNMISIDDQATTLEEYQSLQKETAYAE"
BASE COUNT          860 a          963 c          962 g          958 t
ORIGIN      
        1 gggaaaatgg gagaatggga aaatgggaga atgatgcaga agggagtgtt caattaactc
       61 attttcacat tacctcattt ccacatttct tcatcccgat ggctatcggg gccatacctt
      121 ccaccacacc tttttctcct ccttcggtaa taaatccgca taattcagag gataccgtgg
      181 ttcgataagt ttgttggata ccggtatcgc tttgaccggc aatggttcaa tgaatacagt
      241 tgccggcgga cccggatcag gaggcggcgg ggtctcattc ggcggatcga tcacttcaat
      301 gcgcacttta gccagtcctg cacggatata cccaagtcgt tcagccgcag aataggtcag
      361 gtcaatggac cgtttgttcc attgcggcat ccggtcgttg atgcgcagta tcaccacaga
      421 atcgttggcc ttgttggtga ccattaccca ggtgcccagt ggcaggctct tatgggcagc
      481 ggtcatcttg tcctgggtaa agatctcgcc actggcggtc ttcctgccaa cgaacttgtc
      541 gtggtagtaa ctggccgttc cctcttccac atacgcagac tgggcatcag acaaagagca
      601 gaggcaactc agcaggagca ggcagcttcc tttgacaagg atttgcattc attaaaatta
      661 gccgaatttg gtggtagggt tatccacagg ttcaggatcc gggaaaaagg gaaggctgta
      721 ccccggaaat cagcccttca tgagccagga gccactcttt acggtgtatt ccgcctgcat
      781 aacccgtcag gctgccatca cttccgatca cccggtggca gggaatgagc accagcagcg
      841 ggttggcccc caccgcagcg cccactgccc ggctgtattg gttaccgcca agttgtctgg
      901 ccagcgacag ataagtggtg gaaccgcccg gcggaatacg cagcaatgcc cgccagacca
      961 actttgaaaa atctgaagca gctggcagct cgaaatgagg tagtggggtg ccggggtcgg
     1021 tcaggtacaa cctgaaccag tgggcggcct gttcggtaaa aggatcggat acaccgacag
     1081 gtgcagtatc agaaaaatag caacgggaga ggcttttgcc gtcagattca gccacaaaat
     1141 aaccggcagg agagggaacg cagctgctga acatggctca gatggccact ttcagcttgt
     1201 tgatacggct ctggttgagc ttgctttcgg catactccag gtccacccgg aattccacat
     1261 ccttgttctc tgctgaaggc atatcgaaca tggcatcagt aagcagggct tcacaaatgg
     1321 atcgcaaacc tctggcaccc agcttgaatt ccatggcctt gtcaacaatg aaatccagca
     1381 cttcatcatc gatctccagc ctgatgcctt caatctggaa gagctgtttg tattgcttga
     1441 tcagggcgtt cttgggttca gacaggatgg cacggagtgt ttctgcatcc agcggatcca
     1501 ggtgagtaag cacgggcaga cgaccgatca gttcaggaat gagtccgaat tgcttgaggt
     1561 cctgcggatt gacatactgg agcaggttct ccttgtcaac cacagcttct tcttcctttt
     1621 tgaatccgat cagctgggtg gaggtacggc gggcgatgat cttctggata ccgtcgaacg
     1681 ctcctccaca aatgaagaga atgttctggg tattgatctt gaccattttc tgttccgggt
     1741 gtttgcggcc accctgaggt ggtacgtaca cttcggtgcc ctccagcatc ttcagcaggg
     1801 cctgctgcac accttcaccg gacacatcgc gggtgataga tgggttatcg gtcttccggc
     1861 tgatcttatc gatctcatcg atatagacga tacctctttc ggctgcagcc acatcatagt
     1921 tgcagacctg aaggagacgg gagagaatac tctccacgtc ctcacccaca taaccggcct
     1981 ccgtaaatac ggtggcgtcc acaatggtga aaggaacatt cagacaacgg gcaatggaac
     2041 gtgccagcag ggtcttgccg gtaccggtct cacccaccag aacgatattg gacttttcga
     2101 tctcgacctc ctcgatcttg ggctggttga gacgcttgta atgattgtac acggcgaccg
     2161 acaggatctt cttggcttct tcctgaccta tcacatactg atccaggaac tgcttgatgg
     2221 ccttcggctt catgatggca ttcataccga acttatagcg cttgccggta tgctgcatct
     2281 cttcttctat gagctgctgg gcctgaccca cacaattctc gcagatatgt ccttcctgac
     2341 cggcgatgag aatattcacc tcggttttcg ggcggccaca gaaggaacag tgctggtcgt
     2401 cgttcttttt cattatgcag tttttcgcgg attacgggac agcacctcat ccaccatgcc
     2461 atagtctttg gcttcggcag cggtcatcca gtaatcacgg tcagaatctt cttcaacctt
     2521 gttgaaaccc tgaccggagt gttggctgat aatattataa agttcggctt ttaatttcaa
     2581 agtttccttc agtgcaattt ccatgtcact tgcttgtcct tgcatgccac caagcggctg
     2641 gtggatcatg acacggctat gggtcagggc agagcgtttt cctgcgtgcc cggcacagag
     2701 cagcacagca gccatagagg cggcaatacc ggtacagatg gtggccacat cagggttgat
     2761 atactgcatg gtgtcataga tggccagacc tgaataaaca ataccgcccg ggctgttgat
     2821 gtagatctgt acatcacgct tggagtccac tgagtccagg aataaaagct gggcctggat
     2881 cacgttggcc acatagtcat caatgggtgt tccgaggaag atgatgcggt ccatcatcag
     2941 gcgggagaag acgtccaaag cctgggcgtt cagctgcctt tcttcaatga tatagggcgt
     3001 gaaattggtc acaaactggc tggtactctg catgtaactc tccagcgtag tggtggggat
     3061 acccatgtgg cgggtagcaa acttcttgaa ttcgttattc ggcatatgca gtctcttttt
     3121 gtaaggattg atattcttcc aaagtggttg cctgatcgtc aatgctgatc atgttgcgga
     3181 tcacatccag gaacttttcc tgcatgaccc ggtcgtgcat gctttctaca aatgaacgct
     3241 ctttcagctg actttccaca atataacgca caaaatcttc acccagctgt ccgtagctgt
     3301 tgcgcacatg ctgcacagcg gcctggatga tttcatcctg ggtggcctcc agctgatgct
     3361 cctgctgtac tttgcggaag atcagtgatt ctttcagcct gcgcataaat tgtgagaagg
     3421 tctcttcgtc ttcaccatcg gcagcacggc tgttgatgaa ccatttttcc agaaattgat
     3481 caggcaggtc tatcgaggtc tgttcgatca ggtacttata caaggtattg tctaattgta
     3541 ccctggtcat attgtcatta tactgctcta tgaaaccgct gatgcgttca cgcagggcag
     3601 attcgtcggt tacttcgcca tattctgcag atacagactg gaagaaagct tcattcagtt
     3661 cggccggaat cagtcgcacg atacgatcta ccttacattc aaagccaggc tgaatggctt
     3721 ccacgatctc ttctgacggt tcc
//
LOCUS       DAOMAX010000258         3734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4729_length_3734_cov_4.516173, whole genome shotgun sequence.
ACCESSION   DAOMAX010000258 DAOMAX010000000
VERSION     DAOMAX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4729_length_3734_cov_4.516173"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="PK742_11180"
     CDS             complement(<1..1855)
                     /locus_tag="PK742_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77195.1"
                     /translation="MNRLFAFIMFFMAMTISAAAQMVGGDIFLQGTKVEVGVNHCGAY
                     GSQGAAPVGYHPTNVELGFVADWESDGWETGTPDYCGDYFVPGTPEEGWQVQIGSSTW
                     VNSNQNCSPDDVPGDLIDYTYAGGIYTSVWQGNISSEDMSITQITTLPEDALYFVTRI
                     LLCNDGDTPLEDVYYMRNLDPDNDQPWSFDFTTYNEIVYQPPVDDNALVTSEGLTYGC
                     FLGLGARDTNARVAYGNFFTGDGTPEEVWTGTGGYSGSGSSTADEANSISFYVPVINP
                     GECKCVAFAYILNIDDLEEALEATVTYNLTANGEPVASGDTVIACNPGESVELSVVGA
                     DDYEWTWSPADILDTDIGSTVIANPTETITITALGVGGFCGDASVSITLLIDEDEFAD
                     AGPDDEICIGSSTTLNGDGGDFESGFLWSPATGLSDPTIANPEASPTTTTTYTLITTD
                     IYGCPETDAMTLTVHPLPDVDAGPDQEFCVDGVIVLDASGAVDYEWSPAVGLSDPGIA
                     DPECTVDENTTYTVTGTDANGCVNTDDVFISVNYLPDVDAIASPYTIDVFLGETSTLD
                     VLTGGISFVWSPPTGLSDPNSQNPVVSGIPDTIVYTVTVTDINGCVSTDTVQ"
     gene            complement(1975..>3734)
                     /locus_tag="PK742_11185"
     CDS             complement(1975..>3734)
                     /locus_tag="PK742_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77196.1"
                     /translation="EGDIASENLAITQTTRLPEDKLYFTTEITFCNEGATDLVDVYYN
                     RNVDPDNDQPWSGSFTTVNTIVSQPIEDSVCDALVTSEGQTYGCFLGLGARDYRARVS
                     WGNFSTTAGTPQNVYEGTGGYSSSGTNTNDIANSIAFYIPLIEAGECEIITFAYVLSI
                     DDLEEALDATTAFDLSVNSEVVSSGDTAYYCNPLDSMHLEILGGEDYIWEWSPSTYLD
                     VDTGHIVNFVSPVTQTLIANGVGLCGEVTLEVTLLVDTTVAISDAGDDEPICIGSETT
                     LNGDGGPLDDLYLWVPATGLSDPNIANPVASPTTTTTYTLITTNQYGCPAVDDVTITV
                     NPLPDVDAGPDQEFCVDGVIVLDASGAVDYEWSPAVGLSDPGIADPECTVDENTTYTV
                     TGTDANGCVNTDDVFISVNYLPDVDAIASPYTIDVYLGETSILDVLTGGINFVWSPPT
                     GLSDPNSQNPVVSGIPDTIVYTVTVTDINGCVNTDTVQVNAIGELVVGLPTAFSPNGD
                     GYNDFYAPVLDGSGDLEYFSIYNRWGQLVFESNTATLGWDGMFNGKEAELGSYVVYAR
                     ARTSLDEQREFSGYFTLVR"
BASE COUNT          993 a          945 c          874 g          922 t
ORIGIN      
        1 cctgaacggt atcggtgctc acacaaccgt tgatatctgt tactgttaca gtatagacga
       61 tggtatccgg aataccgctt actacagggt tctggctatt gggatcactg agtcctgttg
      121 gcgggctcca tacaaaactg atacctccgg tcagcacatc cagggtactg gtctcaccaa
      181 gaaaaacatc aattgtatat gggcttgcta tagcatccac atccggcagg tagttcacag
      241 agataaagac atcatcagta ttcacgcagc cgttggcatc ggtaccggta acggtatagg
      301 tagtattctc atccacagta cattccggat cggcaatacc gggatcgctc agtcctactg
      361 caggagacca ttcatagtcc actgcaccgg aagcatccag cacgatgacg ccatctacac
      421 agaattcctg atcaggacca gcgtccacat caggcagcgg gtgcacggtc agggtcatcg
      481 catcggtttc aggacaacca tatatatctg ttgtgataag cgtataggta gtggtagttg
      541 ttggagaggc ctccggattt gctatagtag gatcgctaag accagttgcg ggcgaccata
      601 gaaatcctga ttcaaaatct ccgccatcgc cattcaaggt ggtagaggaa ccaatacaaa
      661 tctcatcatc aggacctgca tcagcaaatt catcctcgtc tatcagaagg gtgatactta
      721 ctgaagcatc accacaaaaa ccgcctacac ccaaagcagt gatagtaatg gtttctgtgg
      781 ggttggctat tacagtcgag ccgatatcag tatccagaat atcagccggc gaccaggtcc
      841 attcataatc atctgcccct accactgaaa gttcaactga ttcaccgggg ttacaggcaa
      901 taacggtatc gccactagca accggttcac cattcgctgt gaggttgtaa gtaacagttg
      961 cttccagtgc ttcttccagg tcatctatat tcaggatata ggcgaaggct acacatttac
     1021 actcacccgg gttgatcacg ggaacataaa aggaaatact attggcttca tcagcagtac
     1081 tggagcctga cccagagtat ccgcccgtac ctgtccatac ttcttccggt gtgccatcgc
     1141 cggtaaagaa atttccataa gccactctgg cgttagtatc cctggctccc agtccgagga
     1201 aacaaccata tgtcagacct tcggaagtta ccagggcgtt atcatctacc ggtggctgat
     1261 agacgatctc attataggtg gtaaaatcga aggaccatgg ctgatcatta tccgggtcca
     1321 ggttacgcat gtaatacaca tcctccaaag gagtatcgcc atcattacaa agcaaaattc
     1381 gggttacgaa gtaaagtgca tcttcaggca aagtagttat ctgagtaata ctcatgtctt
     1441 cagaggaaat attgccctgc caaacacttg tataaatacc gccagcatag gtgtaatcga
     1501 tcagatcgcc gggcacatca tcaggagagc aattctgatt ggaatttacc caggttgaag
     1561 agccgatctg aacttgccat ccttcttcag gtgtacctgg aacaaaatag tcaccgcaat
     1621 agtcaggagt acctgtctcc catccgtcac tctcccagtc ggcaacaaac cctaattcca
     1681 cattggtcgg gtgataaccg acaggtgcag caccctgaga gccataggca ccgcagtgat
     1741 ttacacctac ttctacttta gttccttgaa ggaatatatc tccgcccacc atttgtgcag
     1801 ccgcggagat cgtcattgcc ataaagaaca tgatgaatgc aaacaggcga ttcatactct
     1861 tggtaatttg gtccacaata aatgtcagat aaagataccg ttcacagcag agaattgaaa
     1921 aaaaaagcgc tgttctttga gagaacagcg cttattctgg gtcatttttt tgtcttatct
     1981 caccagggtg aagtatccgg aaaactctct ttgttcatcg agggaggttc ttgctctggc
     2041 atacaccaca tatgagccca gctcggcttc ttttccgttg aacatgccgt cccatccaag
     2101 ggtagcagtg ttgctttcga aaaccagctg gccccagcgg ttatagatgg aaaaatattc
     2161 cagatcgccg ctgccatcca gcaccggggc atagaagtcg ttgtagccat cgccattggg
     2221 tgagaatgcg gtgggaagtc caaccaccag ttcgccgatg gcatttacct gaaccgtatc
     2281 tgtgttcacg cagccgttga tatctgtaac agtgacggtg taaacaatgg tatccggaat
     2341 accgctgacc accgggttct ggctgttcgg atcgctgagt cctgttggcg ggctccatac
     2401 aaagttgata cctccggtca gcacatccag gatactggtc tctcccagat atacatctat
     2461 ggtgtaaggt gaagctattg catctacatc gggaaggtag ttcacagaga taaagacatc
     2521 atcagtattc acgcagccgt tggcatcggt accggtaacg gtataggtag tattctcatc
     2581 cacagtacat tccggatcgg caataccggg atcgctcagt cctactgcag gagaccattc
     2641 atagtccact gcaccggaag catccagcac gatgacgcca tctacacaga attcctgatc
     2701 aggacctgcg tccacatcag gcagcggatt gaccgtaatg gtcacatcat ctacagccgg
     2761 gcaaccatac tgattggtag tgatcagggt ataggtggtt gtggtggtag gacttgcgac
     2821 cgggttggca atattcgggt cactcagacc tgtagccggc acccagagat acagatcatc
     2881 gagcgggcca ccgtcgccat tcagggtagt ttcagaacct atgcaaatgg gttcatcgtc
     2941 gcctgcatca gagatggcga cagttgtatc caccagcagt gtaacttcca gggttacttc
     3001 accgcaaagt ccaacaccgt tcgcaataag ggtttgtgtt accggtgaaa caaaattcac
     3061 tatatgaccg gtatccacat ccaggtaggt agaggggctc cattcccata tatagtcttc
     3121 cccacccagg atctccaggt gcatggaatc aagcgggtta cagtaataag cagtatctcc
     3181 tgaagaaacg acctcgctat taactgataa gtcaaatgcg gtagtggcat ccagggcttc
     3241 ttccaggtca tcaatactca gcacatatgc aaatgtgatg atctcacatt cgccggcttc
     3301 gatcaatgga atataaaatg cgatagagtt agcaatatca ttggtattgg tgccggagct
     3361 gctgtagcca cccgtacctt catacacatt ctgaggtgta ccggcagtgg tactgaaatt
     3421 tccccagctc acacgtgctc tgtaatctct tgccccgaga cccaggaaac aaccgtaggt
     3481 ttgcccttct gaggtaacca gcgcatcgca cacagaatct tctattggct ggctgacgat
     3541 ggtattgaca gtggtaaaag aaccggacca gggctgatca ttatcagggt ccacatttct
     3601 gttgtaatac acatccacca ggtcagtagc accttcgttg cagaaggtga tctctgtagt
     3661 gaagtacagt ttatcttccg gcagcctggt ggtctgtgtg atagccaggt tttcggaagc
     3721 tatatcacct tccc
//
LOCUS       DAOMAX010000259         3733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4733_length_3733_cov_3.673464, whole genome shotgun sequence.
ACCESSION   DAOMAX010000259 DAOMAX010000000
VERSION     DAOMAX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4733_length_3733_cov_3.673464"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /gene="groEL"
                     /locus_tag="PK742_11190"
     CDS             <1..170
                     /gene="groEL"
                     /locus_tag="PK742_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQU77197.1"
                     /translation="LLAAGVIDPVKVTRVALENAASIAGLLLTTEVTIVDKPEPKSAA
                     PAMPGGMDGMM"
     gene            303..1007
                     /locus_tag="PK742_11195"
     CDS             303..1007
                     /locus_tag="PK742_11195"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQU77198.1"
                     /translation="MDTEEKIVSMPRIGDKAPSFTAVTTQGIIHFPEDYEGRWVILFS
                     HPADFTPVCTTEFMTFATMEDEFKALNCDLVGLSVDGLYSHIAWLRTIKEKIVFRDMK
                     NVEVNFPLIEDITMEVSRLYGMIQPGESSTKAVRAVFFVDPAGKIRAMIYYPLSTGRN
                     FHELKRVLKSLQAVDKYNIATPADWEPGEEVIVPPAGSCGTAKERMDGNDEDIRCVDW
                     FFCLKKLQEEEAGAMV"
     gene            complement(1058..1756)
                     /locus_tag="PK742_11200"
     CDS             complement(1058..1756)
                     /locus_tag="PK742_11200"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQU77199.1"
                     /translation="MRTILLYPILLLGVHTLAQTVDVQYQAWLRYQPRWVLNEQWSLK
                     SDIDERVYVFPFRQHQYIFRVQAEYDLGEHWKGSAGGSFFQQYLPQDPDVAVTGTIAE
                     VRPELESNSKYKLTDKWTWQQRFRSDFRFFDGEDGFAYSHTRLRYMTGMKYRISDPFA
                     LTIWDEILVNGMAINEVNFFDQNRIGASLQWKWTNKLAWDLTYFNWYQLRSTPGTYYN
                     RHIVRLALQHQFGQ"
     gene            complement(1797..3200)
                     /locus_tag="PK742_11205"
     CDS             complement(1797..3200)
                     /locus_tag="PK742_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQU77200.1"
                     /translation="MKQQLLAILFCTSAVFGQDSLQTLSHEELLTWVAEHHPLTSSAL
                     LVLDQADAGIMKAKGAFDPTAFAGRYDKYYDDKTYYSLFDAGVEVPLRGPFQLRAGYE
                     QNSGQYLDPENTLPASGLWYGGIEVQLGQGLLIDERRAALQKALIQRELAEQELLLQV
                     NQLLQDASTAYWEWNLAFQKTLLLEEAVQLAADRLDAVRISAVLGDRPSIDTVEAGLL
                     WQQLQIEYQQAEMELTKSRLALSVFLWEDGYTPLLLDPNTVPENEPAIIGQDTPTPVL
                     PAVHPALQSLTFQLEQLEIDRMWQAEKLRPKLKLSYNPLVAANGDIPLAGFSSSNYTW
                     GVGFSMPLFLREARGGLQAVGIYTEQTWLKQQNKTTELQNKSAAYFTEWEVTRSLTDQ
                     YRQTVTGYLQLLQGENTLFLNGESSLFLVNSRQLYWVNGRQKLLELEFKEKQALNNLY
                     GNNANYANWYRSRYLQP"
     gene            complement(3200..>3733)
                     /locus_tag="PK742_11210"
     CDS             complement(3200..>3733)
                     /locus_tag="PK742_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQU77201.1"
                     /translation="TKLQNTFMNYSVRTGLYYITAPQDGYITKAIQTGIGETIKEGEE
                     LLSIMPANPELAAEVYIEPMDLPLLSTGQHIRMQFDGWPAVVFSGWPDMSVGTYGGSI
                     VAIDNFISTNGKYRILVAPDPDSDPWPDGIRVGSGVSAMALLKDVPIWYELWRKVNGF
                     PPDFYRANESGTSEKKK"
BASE COUNT          903 a          928 c          906 g          996 t
ORIGIN      
        1 acctgctggc agccggagtc atcgaccccg ttaaagtgac ccgtgtggca cttgaaaatg
       61 cagccagcat tgccggtctg ctgctcacca cagaggtgac catcgttgac aagccggagc
      121 ctaaatctgc tgctcctgcc atgcccggtg gaatggacgg aatgatgtga tcattttcca
      181 atcgaatcat aaaggccttg ttcacaaaca aggccttttt tatttcctgt caccttccat
      241 tgtaatggat gtcactgttt gtttatcatg tatgcaggaa atttgacttg aaaaaaaaca
      301 atatggatac cgaagaaaaa attgtaagta tgcctcgtat cggcgataag gcaccgtcct
      361 tcacagccgt tactacccag ggcatcattc actttccgga agactatgaa ggccgctggg
      421 tcatcctgtt cagccatccg gcggatttta cgcccgtatg caccaccgag ttcatgacct
      481 tcgccacgat ggaagacgaa ttcaaggcat tgaactgtga tctggtaggt ctgtcggtcg
      541 atggtctgta cagtcacatc gcctggttgc gcaccatcaa agagaagatc gttttccgcg
      601 atatgaagaa tgttgaagtg aacttccccc tcatcgaaga catcactatg gaagtgtcca
      661 ggctatatgg catgatacaa cccggcgaga gcagcacaaa agcggtgagg gctgtattct
      721 ttgtagatcc ggcaggaaag atccgggcca tgatctatta tccgctcagt accggcagga
      781 atttccatga actgaaacgt gtattgaaaa gtcttcaggc tgtagataag tataatattg
      841 ccaccccggc tgactgggaa cccggcgaag aggtcatcgt acctcctgca ggatcatgcg
      901 gtacagcgaa agagagaatg gatggcaatg atgaagatat ccgttgcgtg gactggttct
      961 tttgtcttaa aaaacttcag gaagaagaag caggcgcaat ggtgtaacct gtttatggtt
     1021 tactttgcag aaggggtcgc tcatgcgatc ccttttatta ttgcccgaac tgatgttgca
     1081 aagccagtcg gacaatatgc ctgttgtaat acgtacccgg tgtactgcga agctgatacc
     1141 agttgaagta ggtgaggtcc caggctagtt tgttcgtcca cttccactgc aatgatgccc
     1201 cgatgcgatt ctgatcaaag aagttgacct cattgattgc catcccattc acgagtattt
     1261 catcccagat ggtcagtgca aaagggtcac tgatcctgta tttcattcct gtcatatatc
     1321 gtaaacgtgt gtggctgtag gcaaaaccat cctcgccatc aaagaagcgg aaatcacttc
     1381 gaaaccgttg ttgccaggtc catttgtctg tcagtttgta tttgctgttg gattccagtt
     1441 ccggcctgac ctctgctatg gttcctgtta ctgccacatc cggatcctgg ggcaggtatt
     1501 gctgaaagaa ggaaccgccg gctgagccct tccagtgttc acccagatcg tattctgcct
     1561 gcacccggaa aatgtactgg tgttgcctga acgggaatac atatacccgt tcatctatat
     1621 cactcttcaa tgaccactgt tcattcagca cccacctggg ctgatatctc aaccaggcct
     1681 ggtattgtac gtcaacggtc tgggctaaag tatgcacacc aagcagcagg atagggtaca
     1741 gcagtattgt tcgcatatgc ttgcaaaact acaagctata cagaactggt ctgtgatcaa
     1801 ggttgcagat accggcttct gtaccagttg gcatagttgg cattgttccc atacaaattg
     1861 ttgagcgcct gtttttcctt aaattccagt tccagcaact tttgtctgcc attcacccag
     1921 tatagttgcc ggctgttgac caggaaaagt gagctttcac cattcagaaa aagggtgttc
     1981 tctccctgca gtaattgcag ataaccagta actgtctggc ggtactgatc ggtcagcgaa
     2041 cgggtaacct cccattctgt gaaataggca gcacttttat tttgtaattc ggtggtctta
     2101 ttctgctgtt tgagccaggt ttgttcggta taaattccca cagcctggag gccacctctg
     2161 gcttcccgaa ggaacaaagg catgctgaat cccactcccc aggtatagtt gcttgaggaa
     2221 aatccggcga gtggaatatc gccgttggcg gcaaccaggg ggttatagga tagtttcagc
     2281 ttagggcgta atttttcggc ctgccacatc ctgtctattt ccagttgttc cagctgaaaa
     2341 gtgagcgatt gcagcgcagg atgcacagca ggcagtaccg gagttggggt atcctgaccg
     2401 atgattgccg gttcgttttc cggaacggtg ttcgggtcga ggaggagagg ggtgtaccca
     2461 tcttcccata aaaagacaga caaggcgagg cggcttttgg tcagttccat ttctgcctgc
     2521 tggtattcaa tctgcagttg ctgccatagc agaccagctt ctaccgtgtc aattgatggc
     2581 ctgtctccga gaacagcaga gatcctgact gcatcgagac ggtctgctgc cagctgtaca
     2641 gcttcttcca gcagcaaagt tttctggaaa gccaggttcc actcccagta tgcagtactt
     2701 gcatcctgca acagttggtt gacctgcagt agtaattcct gttcagccag ttctctttgg
     2761 atcaacgctt tctgcaatgc agctctccgt tcatctatca gcagtccctg acccagttgt
     2821 acctctatac ctccatacca taaaccgctt gcaggcaggg tgttttccgg atccaggtat
     2881 tgcccgctgt tttgttcata gccggctctc agctgaaaag gaccacgaag cggtacctcc
     2941 acacctgcat caaacaggct gtaataggtc ttgtcatcat agtatttgtc atatcgcccg
     3001 gcaaaagcag tcggatcaaa agcacctttt gctttcatga taccggcatc tgcctgatcc
     3061 agcaccagca atgcagaaga ggtgagggga tgatgttctg ccacccaggt caacagctct
     3121 tcgtgcgaca gtgtttgcaa actgtcttgc ccgaaaacag ctgatgtaca aaacaaaatg
     3181 gccagtaatt gctgtttcat catttctttt tttcactagt tccggattca ttggcccggt
     3241 agaaatcagg cggaaatccg ttcacttttc tccagagttc gtaccagatc ggcacgtctt
     3301 tcagcagtgc catggcagac acaccacttc ctacacgaat gccatcaggc catgggtcac
     3361 tatccgggtc tggtgctacc agtatccggt acttgccatt cgtgctgatg aaattgtcga
     3421 tggcaacaat gctgcctcca taggtgccta cagacatgtc aggccagccg gaaaagacca
     3481 ctgcaggcca gccgtcaaac tgcatccgga tgtgctgacc tgttgagagt aaaggcagat
     3541 ccataggctc aatatatact tctgctgcca gctcggggtt ggcaggcatg atgctcagca
     3601 gctcttcacc ttctttgatg gtctcgccaa taccggtctg gatggctttg gtgatgtagc
     3661 catcctgtgg cgctgtgatg taatacaatc ctgttctcac agaataattc ataaaggtat
     3721 tctgtaattt ggt
//
LOCUS       DAOMAX010000260         3670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_4870_length_3670_cov_3.912586, whole genome shotgun sequence.
ACCESSION   DAOMAX010000260 DAOMAX010000000
VERSION     DAOMAX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4870_length_3670_cov_3.912586"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="PK742_11215"
     CDS             complement(<1..1580)
                     /locus_tag="PK742_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77202.1"
                     /translation="MKKPVSLLFFVLFYSILFSQEVQWASELVRFSTEYSRTMYSAKQ
                     VLGAPDKLPATGESAVAWAPSTPENPAGEFIHVRFAQPMAIRQVAIGESNISGAVSKV
                     TLIDDNKKKHVVYEDPDIKPSFGYEGGLTHITFPLTDYRVAEVKVELNTKAVAGMNQI
                     DCIGISAAEEPVQVKVFEMETTEEIGPPENLGPQVNSFADDMLPIVSPDGKTLYFARK
                     RHPENVGDEKRDDIWYSKLQADGSWGPAIHMEDPLNNEHHNYVAWVSPDGNSLALAND
                     YKNPTIGQRVSMSVNRNGQWAFPSTLPVNDMYNKNEFSCYHMNTEGNVLLLAIERGDT
                     EGDMDIYVSFKRSNNAWTRPMNIGPVVNTAATEGSVFLAADNRTIYFASNGHSGYGGF
                     DMFVSKRLDDTWTNWTEPINMGSRINTKGDEFYYTVPATGDYLYFSSSSNSYGRADLF
                     RMKLPKELQPDPIAMLKAKLIDRETGEPVIADIEFGGLISEEPRAITTSTNGDVQLIV
                     PRNDYEITIKKQGYIPIST"
     gene            complement(1621..2274)
                     /locus_tag="PK742_11220"
     CDS             complement(1621..2274)
                     /locus_tag="PK742_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77203.1"
                     /translation="MPLFEYIFYLGVIIIVFTTIWKLLASIITSFLKDLGVSKDYSFL
                     FFKAVSYYLLVSITALKTSQQMELTHPVIAVIYAISGTFIIYTTIAANLEKNRWRAVM
                     NFERKRIRVMRYDGYLLMACIVLFILTLFKPQIADTPLHNWILQTIRNIYTTPVLQFF
                     IAIFAFFYMINMIVRGVRATEEVLQTLFIRRQPTAGDQSRTDADGYTDYEEIVDDDQ"
     gene            complement(2418..3101)
                     /locus_tag="PK742_11225"
     CDS             complement(2418..3101)
                     /locus_tag="PK742_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU77204.1"
                     /translation="MDYLYKHMIQAENIHKSYGSLHVLKGLQLTVQAGEVISIMGSSG
                     AGKSTLLHILGTLDQPDSGTLQIAGQDPLKLSAKELARFRNKRIGFVFQFHHLLPEFT
                     ALENVCIPGMIAGRKEDEIQKAAIELLDFLGLSDRQDHKPAALSGGEQQRVAVARALI
                     NKPDIVLADEPSGNLDTHNSRELHELFFRLRDEFHQTFIIATHNEELGNMADRKMVMK
                     DGVFIGSSE"
     gene            3163..>3670
                     /locus_tag="PK742_11230"
     CDS             3163..>3670
                     /locus_tag="PK742_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77205.1"
                     /translation="MKRIIFLLTSFLIVQTSFAGPGDTIKVQTFTFGSPQDAWFVLPD
                     LDVDVEKILMKYTLKCNPDQSPACGEWDYLTYSYLYDHTGQLDSTLLTHANYEVNGQT
                     FDSYAYSTEPVYQYVPSWMYNMVYDDTISLNEYQIGDGAASVSNPLSTSQPLGRTQYI
                     WTADELLAA"
BASE COUNT          831 a          918 c          919 g         1002 t
ORIGIN      
        1 gtgctgatag gaatataccc ctgcttcttg atggtgatct cataatcgtt ccggggtaca
       61 atgagctgca cgtctccatt tgtactggtg gtgatggccc gcggttcttc tgagatgagt
      121 ccgccaaatt caatatctgc aatgacaggt tcgccggttt cccggtcaat gagttttgcc
      181 ttgagcatgg cgatgggatc gggttgcagt tctttcggaa gtttcatccg gaacagatcg
      241 gcacgcccat agctgttgct gctggaagag aaatagaggt aatcgccggt tgcgggaacg
      301 gtataataga actcatcgcc cttggtgttg atacggctgc ccatattgat cggctctgtc
      361 cagttggtcc aggtatcatc cagccgcttg ctcacaaaca tatcgaatcc gccatagccg
      421 ctgtgcccgt tggaagcgaa gtagatcgtg cggttatcag ctgccagaaa gaccgaacct
      481 tcggtggcag cagtgttcac caccggtccg atgttcatgg gccgcgtcca ggcattgttg
      541 ctgcgcttga agctgacata gatgtccata tcaccttctg tatcgcctcg ttctatagca
      601 agcaacaaca cattgccttc agtattcatg tggtaacagg aaaattcatt cttgttatac
      661 atgtcgttga ccggcagggt agaggggaag gcccattggc cgttcctgtt gaccgacatg
      721 gacacccttt gtccgatggt gggattctta taatcattgg ctaatgccag gctgttaccg
      781 tccggactca cccaggccac ataattatgg tgttcgttgt taagtgggtc ttccatgtga
      841 atggcagggc cccagctgcc gtcggcctgc agtttggaat accagatgtc gtcacgtttc
      901 tcatctccta cattttccgg atgtcgtttg cgggcgaaat acagggtctt gccatcgggc
      961 gatacgatgg gcagcatatc atcagcaaat gaattcacct ggggtccgag attctccggc
     1021 ggaccgatct cttccgtcgt ctccatctcg aatactttca cctgtaccgg ttcttccgca
     1081 gcactgatac cgatgcagtc gatctgattc attcccgcta ctgccttggt attgagttcc
     1141 actttgacct ctgccacccg gtaatcggtc agaggaaatg tgatgtgggt gagtccgcct
     1201 tcataaccga aagagggttt tatatccgga tcctcataga ccacatgttt cttcttgttg
     1261 tcgtctatca gggtgacctt gctgacagcc ccgctgatat tgctttcacc gatcgccacc
     1321 tgtctgatgg ccataggctg cgcaaagcgc acatgaatga actcaccagc cggattctcc
     1381 ggagtggagg gtgcccaggc tactgcactt tcgccggtgg caggtagttt gtccggagct
     1441 cccagaacct gtttggcgct gtacatggtc cggctgtatt ctgtgctgaa gcgcaccagt
     1501 tcgctggccc actgcacttc ctggctgaat aaaatggaat aaaacaagac gaaaaagagg
     1561 agggagacag gctttttcat gtgcattaca aacgccggct tcctgcaggt agtatgccat
     1621 ttattggtca tcatctacga tctcttcata atcagtatag ccatcggcat ccgttcttga
     1681 ctgatcacct gcggtaggct gtctgcgaat gaacagggtt tgcagcactt cttctgtggc
     1741 cctgacaccc cggacgatca tgttgatcat ataaaagaag gcgaagatgg cgatgaagaa
     1801 ctgcaatacc ggagttgtat agatgttgcg gatggtttgc aggatccagt tgtgcaaagg
     1861 ggtatctgct atctgcggtt tgaacagagt cagaatgaac agaacgatgc aggccattag
     1921 caggtagccg tcataccgca ttaccctgat ccttttgcgt tcaaaattca tgactgcccg
     1981 ccagcggttc ttttccaggt tggctgcgat ggtagtatag atgatgaacg tgccgctgat
     2041 ggcatagatg accgctatga ccggatgggt cagttccatc tgctggctgg tctttagtgc
     2101 cgtgatactt accagcagat agtaactcac tgccttaaag aacaggaaag aatagtcttt
     2161 ggatacaccc aggtctttca ggaaagaagt aatgatactg gccagcaact tccagatggt
     2221 ggtgaacaca atgatgatga cacccaggta aaatatgtat tcaaatagtg gcaagagggg
     2281 gattcgttgt tcgttgatcg ttaatcgttg ttcgttattc gttatgcgtt attcgttttt
     2341 ggttgttggt gggaggggag tgttcaatta actgttatct cattactcaa tacccactac
     2401 ccactactca ctactcacta ctcactactt ccaatgaaca ccccatcttt catcaccatc
     2461 ttcctgtcgg ccatgttacc cagctcctca ttgtgcgtgg ctatgatgaa ggtctgatgg
     2521 aattcatctc tcagccggaa gaacagttca tgcaactcac ggctgttatg cgtatccagg
     2581 ttgccggaag gttcatctgc cagtacgata tccggtttgt tgatgagtgc tctggctacg
     2641 gccactcttt gttgttcacc gcccgacagg gctgcaggct tatgatcctg cctgtctgac
     2701 agtcccagaa agtccagtag ttctatggca gctttctgga tttcatcctc ttttctgccg
     2761 gcgatcattc cgggaatgca aacattctcc aaagctgtga actccgggag caaatggtga
     2821 aactggaata caaatccgat gcgcttgttt cgaaaccggg caagttcctt agctgaaagc
     2881 ttcagcggat cctgacctgc gatctgcagg gtgcctgaat cgggctgatc cagggtgcct
     2941 aagatgtgta gcaaggtgct ttttcccgct ccgctggatc ccatgatgct gatgacctct
     3001 ccggcctgta ccgtcagttg gagaccttta agtacatgca atgaaccgta agatttgtgg
     3061 atgttttcag cctgtatcat gtgcttgtaa aggtagtcca tcaggggcat atcagatcag
     3121 acgaacgggc atgccttata tttgctgttc tatatctcac atatgaagcg tattattttt
     3181 ctgttaacct cattcctgat cgttcagaca agctttgccg gaccgggaga taccatcaaa
     3241 gtgcagacct tcaccttcgg ctctccgcag gatgcctggt tcgttttacc agaccttgat
     3301 gtggatgtgg agaagatcct gatgaaatac acgcttaagt gtaatccgga tcagagtcct
     3361 gcctgcggcg aatgggatta cctgacctat tcctacctct atgatcatac cgggcaactt
     3421 gacagcaccc tgcttaccca tgccaactat gaggtgaacg gacagacatt cgatagctat
     3481 gcctattcca cagaaccggt ttatcagtat gtgccatcct ggatgtacaa catggtctat
     3541 gatgatacca tctctctgaa tgaataccag atcggcgacg gagcagcatc tgtcagcaat
     3601 ccgctctcca cctcccagcc gcttggtcgc acccagtata tctggaccgc cgatgaactg
     3661 ctggcagcag
//
LOCUS       DAOMAX010000261         3592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5033_length_3592_cov_3.407125, whole genome shotgun sequence.
ACCESSION   DAOMAX010000261 DAOMAX010000000
VERSION     DAOMAX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5033_length_3592_cov_3.407125"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PK742_11235"
     CDS             complement(<1..675)
                     /locus_tag="PK742_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77206.1"
                     /translation="MKNLIRYHVIPKTFLLFILIWTVSVQAQVPAELSQRNYGGNDLE
                     YENTILPLDNGQLLVACESGTGINGDKTEDYGGYDIWIYKLDTDLNIIWDHTYGGDFS
                     DLDMLVRKAPDGGYYVACSSLSDVSGNKTVPSFGSYDVWVFKIDSIGNIQWQTALGGD
                     KSDLFKSMIVTSDGDLLIASYTFSGISGNKTVDSVGGYDIWLVKLDSMGNQIWQSRAG
                     SDGWDII"
     gene            complement(672..>3592)
                     /locus_tag="PK742_11240"
     CDS             complement(672..>3592)
                     /locus_tag="PK742_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77207.1"
                     /translation="LILICSQLAFSQVPDILWQKGYGGSSYEGKVNIVQTLSGGYLVV
                     QESNSPVYADKSTVYGGFDIWVLCLDQNGDILWENTYGGVSDELDPYVAADSDGSFYI
                     ACNSTSGISGVKESANYGSYDIWILKIDSLGDIIWQKNMGGNAADEIGDLIATSTGDV
                     LLSSNSSSPVSGNKTVGTNGSYDIWLVKLDSGGNEIWQSGAGTSSVDAMYGNAALERP
                     NGNYMICTSTSGGISGDKTLASKGSNDFWMIEFDTSGALVWQNVVGGSAYDGGGIVIN
                     TDDDGFLISGLSYSDISGDKTENSRGLDDMWLVKCASDGSIEWDKTIGGATSDNASGN
                     VVQWPDGSFVILGLSQSFISGDKTEASYGSFDYWMVRIDSARNILWQKTYGGIGGEYY
                     ATFLSLDDYSILIAGMSQSGISGNKTVSLSGASDMWLIQLEPDSICSDTEITIDSVLY
                     DTTLTLIDGTVADTSGTYVVVLSGDGCDSIITYNLEFYFTPVVNFTYAADLTELTFTD
                     LSENDPTSWKWFFGDGTTSTEQNPVHTYATAGSYGVCLKATNDAGTAFDCAWIEVNAA
                     GAPVADYTFETVEDSTYFTDMSSPEATSWLWFFGDGTTSSEQNPVHAYASSGSYAVCL
                     LAGNDDGSSYYCDIIQICVPVTITISGDSAICSPGSTTIISDLDEATAFMWRKDGLPY
                     PSAGATYNMIEVDEPGSYALTYIDPEGCTGTTNPVTVALSDPPEVTLILPDDQFCKFD
                     GLIALSGGLPAGGIYSGPGISGGNLNTGSLAAGTYELTYSYINEAGCSATATDTFYIS
                     NNPPASATLGSASNNLCVNDPVVFFANSGAGYTYQWYRNNTLLPGETALAYLASTPGN
                     YQVEVTNAEGCSRLSAKKKVVSSGCRMAEIEGAELLLYPNPADDVVHYLISFTGQTGQ
                     SCDIRLRDLTGRLLLERFELITGQQISGFIALDLPAGWYTISVQTENGYLLTNPLIIE
                     "
BASE COUNT         1011 a          922 c          743 g          916 t
ORIGIN      
        1 aatgatatcc cacccgtcag acccggcccg actttgccag atctgatttc ccatactatc
       61 caacttcacc agccagatat cataccctcc taccgaatca acggtcttgt tgcccgatat
      121 cccactaaaa gtatatgatg caattagaag atcaccatct gaagtcacaa tcatggactt
      181 gaacagatct gatttgtcac ctcccaaagc agtttgccat tgtatattac cgatcgaatc
      241 gatcttaaat acccatacgt catagctgcc gaaagaagga actgttttat ttcccgacac
      301 atctgacaag ctggaacacg ccacataata gcctccgtcc ggagcttttc ttactagcat
      361 atccagatca ctgaaatcac caccatacgt atgatcccaa atgatattga ggtcagtatc
      421 cagtttatag atccagatat cataaccacc atagtcttcc gtcttatcgc cattgatacc
      481 cgttcccgat tcacatgcca ccagcagttg gccgttatca agtggtagga tcgtgttctc
      541 atattccaga tcattcccgc cataatttcg ttgagaaagt tcggcaggaa cctgcgcttg
      601 aacagacacg gtccatatca aaatgaacag caaaaaagtt ttcgggatca catgatacct
      661 aatcagattc ttcattctat gatcaaaggg ttggtcaaca aatatccatt ttctgtctgc
      721 acagaaatgg tataccagcc tgccggcaga tcaagtgcaa tgaacccgct gatctgctga
      781 cctgttatca attcaaaccg ctccaacaag agtcttccgg taaggtcacg aaggcggata
      841 tcacaactct gaccggtctg cccggtaaat gaaataaggt aatgcaccac atcatcggca
      901 ggattgggat acaacaataa ctcagcaccc tcaatctcgg ccatccggca tccgctgctc
      961 accactttct tcttggctga taatctggag caaccttcgg cattggttac ttctacctgg
     1021 taattacccg gagttgatgc caggtaagca agtgctgttt ctccgggcaa cagggtgtta
     1081 ttgcgatacc actgataggt ataaccggca ccgctgttag caaaaaacac cacaggatca
     1141 ttcacacaca gattattact ggcagagccc agggtggcac tggcaggtgg attattactg
     1201 atataaaaag tatctgttgc agttgcgctg caacctgctt cattgatata ggaataggtt
     1261 agttcataag tacctgctgc cagtgatccg gtgttgaggt ttccgcctga tatgcccggt
     1321 ccgctataga tacctcctgc aggcagacct ccgctcaggg cgatcagtcc gtcaaacttg
     1381 caaaactgat catcgggcag gatgagtgta acttctggtg gatcagataa agcaaccgtt
     1441 acagggttag ttgtacctgt gcatccttcc ggatcgatgt aggtcagtgc gtagcttcca
     1501 ggttcatcta cttcgatcat attatatgtg gcacctgcag acggataggg caatccatct
     1561 tttctccaca taaaggcggt ggcctcatcc aggtctgaga taatagtggt agagccggga
     1621 gaacaaattg cagaatcgcc ggagatggtg atggttaccg gaacgcaaat ctgaatgata
     1681 tcacaatagt aggatgagcc gtcatcattt cctgccagca gacaaaccgc ataactacca
     1741 gatgaagcgt aggcgtgaac agggttttgt tctgatgaag tggtaccatc gccgaaaaac
     1801 cacagccagc tggttgcttc aggcgatgac atatcagtga aataggtgga gtcttcaaca
     1861 gtttcaaagg tataatcagc cacaggtgcc ccggcagcgt tgacctctat ccatgcacaa
     1921 tcaaatgcag tgccagcatc attggtggcc ttcagacaca ccccgtagct tccagctgtt
     1981 gcataggtat gtacagggtt ttgctcggta gatgtggtac catctccaaa gaaccatttc
     2041 cagctggtgg ggtcgttctc agagagatct gtgaaggtca attctgtgag atctgctgca
     2101 taggtgaagt tgaccacagg tgtaaaatag aactccaggt tataggtaat gatgctgtca
     2161 cagccatcgc ctgataatac cactacatag gttccggagg tatctgccac agtgccatca
     2221 ataagggtca atgtggtatc gtacaatact gaatcaatgg tgatctctgt atcagaacaa
     2281 atggaatccg gttcgagctg aatgagccac atatcggatg cgccacttaa tgaaacagtc
     2341 ttattacctg aaattcccga ctgcgacata ccagctatga gaatgctgta atcatccaaa
     2401 gaaaggaagg tagcatagta ttcgccacca atacctccat aggtcttttg ccacaggata
     2461 ttcctggcag aatcaatgcg caccatccag taatcaaagc tgccataact ggcttctgtt
     2521 ttatcgccgc tgataaaaga ctgagacagg cccagtatta caaaacttcc atcaggccat
     2581 tgtactacat tcccggaagc attgtctgaa gtagcaccac cgatcgtctt atcccactca
     2641 atggagccat ctgaagcaca cttgaccagc cacatatcat ccaggcctct ggaattctct
     2701 gtcttgtctc cgcttatatc agaataggat aatcccgaaa taaggaatcc atcatcgtct
     2761 gtgttgatca ctattcctcc accatcataa gcacttccac caaccacatt ttgccagacc
     2821 aatgcgccgg aagtgtcaaa ctcaatcatc cagaaatcat tggaaccttt gctggcaagg
     2881 gtcttatcgc cggatatgcc gcctgatgtg gaagtgcata tcatataatt tccatttggc
     2941 cgctccaatg cggcattacc gtacattgca tcaaccgaac tggtaccagc tccagactgc
     3001 cagatctcat tgccaccact gtccagtttc accagccaga tgtcgtacga cccattggtg
     3061 cccactgttt tgttgccact aaccggcgat gatgaattag aggacaacag cacatcgcct
     3121 gtactggtag cgatcagatc tcctatctca tcagcagcat tacctcccat atttttttgc
     3181 catataatat cacccagaga atcgatcttt aatatccaga tgtcataaga tccatagtta
     3241 gcactctcct ttacaccaga aataccagag gtagagttac atgctatata aaatgatccg
     3301 tcagagtctg cagccacgta aggatcaagt tcatctgata ctcctccgta ggtattctcc
     3361 cagagaatat ctccattctg atccaggcat aaaacccaga tatcaaaacc tccatagacg
     3421 gtacttttgt cggcatatac cggagaattg ctctcctgaa ccaccagata tccgccgctg
     3481 agagtctgaa caatatttac ctttccctca taggaagaac ccccatatcc cttctgccag
     3541 agtatatcag gtacctgcga aaaagcaagt tgcgaacaga tcaggatcaa aa
//
LOCUS       DAOMAX010000262         3585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5048_length_3585_cov_3.963173, whole genome shotgun sequence.
ACCESSION   DAOMAX010000262 DAOMAX010000000
VERSION     DAOMAX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_3585_cov_3.963173"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3229)
                     /locus_tag="PK742_11245"
     CDS             complement(<1..3229)
                     /locus_tag="PK742_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77208.1"
                     /translation="MTHKYLRWQLLLWLVLLSFWGQAQIVGTNAFLQGNYAEVGVNTC
                     GAFASNAAPPAGYVFTGLSGLNFVADSDLDGWDAGTPNYCGDYAIPGSPVEGWGIQLD
                     GVSYYNTDLGCGVSSIPGEITAYNAAGDSVEVIWQGTLSGVTITQRSVLHADDLYFVT
                     WVTLSNESGSDFTDVYYRRNIDPDNEQLATGSYTTINTVEANPFMGDPYSIVTGIGET
                     YGCYLAIVANNLFSMASRGNFGTTSGLSVSDSYTGLSGFFLDGTATEDAAIQMSFWVP
                     ALPNGGSATFAFAYVFSEDAVEDALEDTYITSDESTDVGIISYDAPETGCGLGLEAVT
                     VNMYNYGFEPQSDIPVFYQVDGGAVVSETIAGPVDPGTFAEYTFTALADLSAVGDHTI
                     SAWTELPGDTVLYNDTADVVITNVPVISGFPYTEDFEGGSGGWTSGGTNSSWELGYPS
                     ASIIDGPPPGTPSSENSWATALYDYYNISEDSWVKSPCFDFSSLAFPYVKFDIWWETQ
                     DFWDGARLEYSLDAGASWTAIGGVGTGDNWYPSGGCFSFGFDPFTGIYYPAWDGSSGS
                     WKTAVHDLSFLAGEPQVQLRMHFKSYVFTPFYDGIAFDNFFVGDPYDNDIGVVALIDP
                     SSGPALGAAEAVTVQIENFGVLDQSGFNVSYQMDGGPVVTELFTGTVTGGGGTATHTF
                     ATTEDLSADGDYTFVAWTELGTDEDLSNDTLIQVVSNLLPITGTDGYYIYSNVYGGPE
                     PWFTTSGQETMDAVYGPGEWTLGFYETLDPEAVFSTSTCFVWMEGGDAMASELETFFD
                     NNTVLIENWVASGGHLFVNSAPNEGDGMSFHFGGVNLFYAYYTNTAEAFDASHPIFDG
                     PFTPVGTSWSGGSFGHAVIQDGGFDTVIVDQFSPSQVVLAEKGWGAGRVMFGGMTPFA
                     FHSPLAEATNLRYNIVSYLASCVLSDIDLGIASIIDPANGCGLGDEAVTVKIRNYGGV
                     PQSDIPVNYQLDGGAVVSETWIGTLDPGETVEYTFTALGDFSALGDHDLVAWTSFPGD
                     TIPGNDTADAVITNIPVVDVYPYLEAFEAGSGGWTSGGTNSTWAQG"
BASE COUNT          990 a          909 c          871 g          815 t
ORIGIN      
        1 ttccctgagc ccaggtagag ttggttccac ctgaggtcca tccgcctgaa cctgcttcaa
       61 atgcttcgag gtaaggatac acatcaacga ccggaatatt ggtaatgacc gcatcagcag
      121 tatcatttcc ggggatggta tctcccggga aagaggtcca tgcaacgagg tcatggtctc
      181 caagggcaga gaagtcgccc aaagctgtaa aggtgtactc aactgtttca cccggatcca
      241 gtgtacctat ccatgtttca gaaaccacag caccgccatc cagctggtag ttgacgggta
      301 tatctgactg tggtacacca ccgtagttgc ggatcttcac ggtaactgct tcatcaccca
      361 aaccacaacc attagcagga tcaatgatag aagcaatacc cagatcaata tcgctgagca
      421 cacaggaagc gagataagaa acgatgttgt atcgcaggtt tgttgcttcc gcaagcggac
      481 tgtggaatgc gaacggtgtc ataccaccga acattacacg acctgcaccc catccttttt
      541 cggcaaggac aacctgtgat gggctgaatt gatctacgat aaccgtatca aatccgccat
      601 cctgaatgac agcatgtcca aagctgccac cactccagct ggtaccaaca ggcgtgaacg
      661 gcccgtcgaa aataggatga gaagcatcaa aggcttcagc agtgttggtg taataagcat
      721 agaacagatt cacaccacca aagtggaagc tcattccatc accttcattc ggagcggagt
      781 tcacaaatag atgtccgcct gatgccaccc agttctcgat cagaacggtg ttgttatcaa
      841 agaatgtttc cagttcactg gccatggcat cgccaccttc catccacaca aagcaggtgg
      901 aggtggagaa cacagcttca ggatccaggg tctcatagaa accaagggtc cattcgcccg
      961 ggccgtacac agcatccatg gtttcctgtc cgcttgtagt gaaccagggt tcaggtccgc
     1021 catatacatt ggaatagata taatagccat ctgttccggt aatgggcaac aggttggaaa
     1081 ccacctggat cagggtatca ttagagaggt cttcgtcagt gcccaattct gtccatgcaa
     1141 cgaaggtata gtcgccgtct gcactcaggt cttctgtagt ggcaaaggta tgagtagcgg
     1201 tacctccacc accggtcacg gtaccggtga agagttctgt aacaacagga ccaccatcca
     1261 tttgatagct cacgttgaat cctgactgat cgagcacgcc aaagttttcg atctgcacag
     1321 ttacggcttc tgctgcaccc agtgcagggc cggagcttgg atcgatcagg gcaactactc
     1381 ctatatcatt gtcataagga tcacccacaa agaagttatc aaaggcgata ccgtcataga
     1441 acggagtaaa tacatatgat ttaaagtgca tgcgtaactg cacctgtggt tcgccggcca
     1501 ggaagctcag gtcatggaca gctgttttcc agcttccgct gctgccatcc cacgccggat
     1561 aatagatacc ggtaaacgga tcaaatccaa aggagaagca accaccggag ggataccagt
     1621 tatcgccggt tcccacaccg ccaatggcag tccaggaagc acctgcatcc aatgagtatt
     1681 ccagacgtgc accatcccag aagtcctggg tttcccacca gatgtcaaac ttgacatatg
     1741 ggaatgccag gctggagaag tcgaagcagg ggcttttcac ccagctgtct tcgctgatat
     1801 tataatagtc atacaaggca gtggcccagc tgttctctga tgacggggtt cccggaggag
     1861 gaccgtcaat gatggaagca gaagggtagc ccagttccca tgaactgttg gtacctccgg
     1921 aggtccatcc accggagccg ccttcaaagt cttcggtata agggaagcct gaaatgaccg
     1981 gaacattggt gatgacaaca tctgcagtat cattgtacaa cacagtatct cccggaagtt
     2041 cggtccaggc agaaatggta tgatcaccaa cggcagaaag atcagccagg gcagtgaaag
     2101 tatattcagc aaaagttccg ggatcaaccg ggcctgcgat ggtttcgctt actacggcac
     2161 cgccgtccac ctgatagaat acaggaatat ctgactgcgg ttcaaaacca taattataca
     2221 tgttcacagt tacagcttca agacccaggc cgcaacctgt ttcaggagcg tcataactga
     2281 tgatacctac atcggttgat tcatcgctgg tgatataggt atcctcgagc gcatcttcta
     2341 ctgcatcttc gctgaacaca taggcaaatg cgaaagtagc tgaacctccg ttgggaagag
     2401 cgggaaccca gaaactcatc tgaatagcag catcttcagt ggcagtacca tccaggaaga
     2461 aacccgaaag tcctgtgtag gagtcagaaa cagaaaggcc ggaggtagta ccaaagttac
     2521 cgcgactggc catagagaat aggttgttgg caacgatagc cagatagcaa ccataggttt
     2581 caccaatacc tgtcacgatg gaataaggat ctcccatgaa aggatttgct tccacggtat
     2641 tgatggtggt gtaggaaccg gttgccagtt gttcattatc cgggtcgatg tttctgcggt
     2701 aatacacgtc agtaaaatct gagccggatt cgttgctcaa agttacccag gtaacaaagt
     2761 acaggtcatc tgcatgcaag acagaacgtt gggtgatagt tacaccactc agtgtaccct
     2821 gccagatcac ttcaactgaa tcgccggccg cattgtatgc agtgatctct ccgggaatac
     2881 tgctgacacc gcagcccaga tctgtattgt aatagctcac tccatctaac tggatacccc
     2941 agccttctac cggagaaccg ggaatggcat agtcaccaca gtagttggga gtaccggcat
     3001 cccagccgtc aaggtctgag tctgccacaa aattcaagcc ggaaagaccg gtaaaaacat
     3061 agcctgccgg aggtgcggca ttagatgcaa aggcaccaca ggtgtttacg cctacttcgg
     3121 cgtagtttcc ctgcaggaat gcatttgtgc ccacaatctg cgcctgcccc cagaatgaaa
     3181 ggagaacaag ccacagcagc agttgccatc gaaggtactt gtgcgtcata aaattggttt
     3241 aaggatcaat gaatagtctg taggatccat gtttccgttc cgcatcctgt accatctggt
     3301 tcatatcagg ttcaggcagg agtccggttc atgaaatctg ctcctaaaag tagtaaagaa
     3361 ttgaaaaaac cgctaaaatg actttgtaac aactgtaata aaaaaaaagg cggccgtttt
     3421 ggccgccttt tttgacaaaa tgataaaact atttggtcag tactacaggc ttgctgaacc
     3481 ggttatcgcc tgcattgatc catagtgtat agaccccgtc agccaggtta gacagatcca
     3541 gttcgatggt tttgtactgt accattccgg gcagtgcgct ccaga
//
LOCUS       DAOMAX010000263         3569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5092_length_3569_cov_4.749004, whole genome shotgun sequence.
ACCESSION   DAOMAX010000263 DAOMAX010000000
VERSION     DAOMAX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_3569_cov_4.749004"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PK742_11250"
     CDS             complement(<1..1091)
                     /locus_tag="PK742_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77209.1"
                     /translation="MRYLFLLLGFVLTLQACTLTENIQDGQTAWNLKKYGLAAQLLEK
                     EFSKEDPANDNGALAFQIGRCYLYTGNSSAAASWFKKAIEWEYGSEAILQYAFTLKRL
                     EQYDDAIEQFNEYLKEEPYRRPEINVEINACEQAIAWKARQEDEFERDTYVSNIRVLN
                     SPDADFQLVHAADDLVLFTSSRASATGENKDSWTGNQYYDLFQSTTDRPGSFAVPIPW
                     EAGMNTPYNDGSVVFNSNYSEMYLVRCGSDNKKIDDYCSIYVSYRQPGDTWTEPLELK
                     FFEDSLNLGMPCLSPDDQTLFFIATDPNGEGGSDIYFAKRLLDGWDIPQNAGTSVNSE
                     GNEAFPTFGPDGTFYFSSDGLPGMGGLDI"
     gene            complement(1185..1619)
                     /locus_tag="PK742_11255"
     CDS             complement(1185..1619)
                     /locus_tag="PK742_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77210.1"
                     /translation="MVKQFALPSYVIWIFAGVAFVSAGTLAVFEWTFGLGAVVLAGIV
                     AYIGYRFFFRPAFIEMDIMKSGEPATAVILESWDTGRKINDNPRLGLKLEVRPPEMVP
                     YQVEVQHNIKEEERRMFREGRVLHVKIDSRNPKKVAILPKAS"
     gene            1859..2872
                     /locus_tag="PK742_11260"
     CDS             1859..2872
                     /locus_tag="PK742_11260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQU77211.1"
                     /translation="MNGGWVPDFIIRRRIRKLIQQRLDEETMHGGQPDYTAGILQAMR
                     SGPVAIHTEAANEQHYELPPPFFREVLGSQMKYSCCYWDDHTKTLDMAEEAALSLTIQ
                     RAGLQDGMRILELGCGWGSLTLAMARRFPDARITAVSNSAPQRQYIQNRCVAEGIDNV
                     RIITADMNEFTPDKQYDRVVSVEMFEHMRNWETLLQRIHSWLKPDGKLFIHIFTHKRF
                     TYFYEYKDETDWMSKYFFTGGIMPSESLMSELNNPFRIEEQWTWSGTHYQKTAEEWLK
                     NMTRSRGDVLPVLEETYGRSELKKWWVYWRVFFMSCAELWGYNGGKEWTVSHYLLSKN
                     SQV"
     gene            2869..>3569
                     /locus_tag="PK742_11265"
     CDS             2869..>3569
                     /locus_tag="PK742_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQU77212.1"
                     /translation="MKQRIAVVGTGIAGMSAAWWLHQDHDITVFEAEQRIGGHTNTAF
                     IQEGEKTVPVDTGFIVCNEQNYPLLMKLFRHLDVPLKPTSMSFSVRHLADDLEYCGSS
                     LELLFVQRRNLFSWRYLRFLRQVQRFNEECTEVLQDQSLHDLSIAEYAASKGLGRDFL
                     HWYILPMSSALWSTPYHVTLDFPVLSLVRFFHNHGLLGLHTQYQWYTVEGGSEVYKQK
                     LIAPFKDRIHTGLGVT"
BASE COUNT          934 a          810 c          900 g          925 t
ORIGIN      
        1 aagatatcca gcccgcccat gccgggaagg ccatcggaag aaaaatagaa tgtgccatcg
       61 gggccaaagg ttgggaaggc ttcgttgcct tcgctgttga cagaggtgcc ggcgttctgc
      121 ggaatatccc atccatccag caggcgtttg gcaaagtaga tatcgctgcc gccttcaccg
      181 ttgggatctg tggcaatgaa gaagagggtc tggtcatcgg gcgacagaca aggcataccc
      241 aggttcaggc tgtcttcgaa gaatttgagc tccagtggtt cagtccaggt atcgccgggc
      301 tgacgatagc ttacatagat ggagcagtaa tcatcgatct ttttgttgtc tgatccgcag
      361 cgcaccagat acatttcaga ataattgctg ttgaatacca cagaaccatc attataagga
      421 gtgttcatgc cggcttccca gggaatcgga accgcaaagg aaccaggtcg atctgtagtg
      481 ctttgaaaga ggtcataata ctgattgccg gtccaggaat ctttattctc gccggtggca
      541 gatgctcggg aagaagtgaa caggaccagg tcatctgcag catgtaccag ctgaaagtct
      601 gcatcgggag agttcagcac cctgatgttg ctaacgtagg tatctctttc aaattcgtct
      661 tcctgccggg ctttccaggc aatggcctgt tcacaggcat tgatctctac gttgatctct
      721 ggtcggcggt aaggttcttc tttcaggtat tcattgaact gttcgatagc atcgtcatac
      781 tgctccaggc gcttgagggt aaaagcgtac tgcaggatgg cttcagaacc atattcccat
      841 tctatggctt ttttgaacca gcttgccgca gcactgctgt ttccggtata gagataacac
      901 cggccgatct gaaaagccag ggcaccgttg tcattggcag gatcttcttt gctgaattcc
      961 ttttccagca actgggctgc caggccatac tttttaaggt tccaggcagt ttgtccgtcc
     1021 tgtatgtttt ctgtcagggt acatgcctgc agggtcagca caaaaccaag tagcagaaac
     1081 aggtatctca tagccataaa ggtacatgat aaacgacggc tgaccggctt tgttttggca
     1141 ggaaacggtc aaaaaaaacc accctttcag gtggttggat catttcagga tgccttcgga
     1201 agtatggcca ctttctttgg attgcggctg tcaatcttga cgtgcagcac ccgaccctca
     1261 cgaaacatcc tgcgttcttc ttctttgata ttgtgctgaa cttctacctg ataaggcacc
     1321 atttccggcg gacgtacttc aagtttcaga cccagccttg ggttgtcatt gatctttctg
     1381 ccggtatccc agctttccag aataacagct gtagccggct cgccgctttt cataatgtcc
     1441 atctcaataa aggcaggtct gaaaaagaac cggtatccga tataggcaac tataccagcc
     1501 agcacaactg cgcccagtcc aaaggtccat tcgaaaacgg ccaatgttcc ggcggataca
     1561 aatgccactc cggcaaagat ccagatgaca taagaaggta gggcaaattg tttgaccatg
     1621 gcagaggagt tgtatattat acgttagaca gcaataaaaa gttcagctat gatgccactt
     1681 tctgcttttt ttcacataat attttacgat ctgaaaatta cattcatctg agccccttgc
     1741 cataacaatt gcatttggaa tgtgactgct gccatttgag aggttttgtc tttttttgta
     1801 ctactgaaca aaacagcccg atggggtttt ctgacaaatg gcaataacat cagcattatt
     1861 gaacggtggg tgggtacctg actttattat aagacgccgc atcaggaagc tcatccagca
     1921 gcgactggat gaagagacca tgcacggtgg tcagccggac tatacggcag gcatcctgca
     1981 ggctatgcgc agtggtccgg ttgccataca tacagaagca gccaatgagc agcactatga
     2041 gctgcctccg cccttctttc gcgaggttct tggcagccag atgaaataca gctgttgtta
     2101 ctgggatgat cataccaaaa cactggatat ggcagaagag gctgcattaa gcctcaccat
     2161 acagcgggca ggtctgcagg atggaatgcg tattctggaa ctgggttgtg gctggggaag
     2221 tctgacgctt gccatggcaa ggaggttccc ggatgccagg atcactgcgg ttagcaactc
     2281 tgcaccacaa cgccagtata ttcaaaacag gtgtgtggca gaaggaatag acaacgtgcg
     2341 catcattacc gcagatatga atgagtttac tcctgacaag caatatgacc gtgttgtcag
     2401 tgttgagatg tttgaacata tgcgaaactg ggagacattg ctgcagcgga tccactcctg
     2461 gcttaagccg gatggtaaac tgttcattca catctttacc cataaacgat tcacctactt
     2521 ctatgaatac aaagatgaaa ctgactggat gtcaaagtat ttctttacag gcggaatcat
     2581 gccttcagaa tcactgatgt ctgaactgaa taatccgttc cggatagaag aacaatggac
     2641 ctggtctggc acacactacc agaaaacagc cgaagaatgg ctaaagaata tgacccggag
     2701 caggggtgat gtgctgcctg tgctggaaga gacttacgga agaagtgaac tcaagaagtg
     2761 gtgggtttac tggcgtgttt tctttatgtc atgtgctgaa ctttgggggt ataacggtgg
     2821 aaaggaatgg accgtgagtc actatctgct gtcaaagaac agtcaggtat gaaacaacga
     2881 attgcggtag ttggcactgg tatagccggc atgtctgctg cctggtggct tcatcaggac
     2941 catgatatca cggtctttga ggccgaacaa cgtataggcg gacacaccaa tacggcattt
     3001 atacaagagg gcgaaaagac tgttccggtt gataccgggt tcattgtttg caatgaacag
     3061 aactatcccc tgttaatgaa attgttcagg catctggatg ttccgttgaa gcccacgagc
     3121 atgtcattca gcgtcaggca tctggcagac gatctcgaat actgcggaag cagtcttgaa
     3181 ttgttgttcg tgcaacgtcg taatcttttt tcctggcgtt atctgcgatt tctcagacag
     3241 gtgcagcgtt ttaatgaaga atgtacggag gtgctgcaag atcaaagcct gcatgatctc
     3301 agtattgcag aatatgcagc aagtaaaggg ctgggaaggg attttctgca ctggtacata
     3361 ctgccaatga gcagtgcact gtggagcaca ccttatcatg ttacccttga ttttcctgtg
     3421 ttatctctgg ttcgcttttt tcacaatcac ggattattag gactgcatac acaatatcag
     3481 tggtacactg tagaaggagg cagcgaagtg tacaaacaga aactaatagc accattcaaa
     3541 gaccggatcc ataccggatt gggtgtaac
//
LOCUS       DAOMAX010000264         3567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5099_length_3567_cov_4.462699, whole genome shotgun sequence.
ACCESSION   DAOMAX010000264 DAOMAX010000000
VERSION     DAOMAX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5099_length_3567_cov_4.462699"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK742_11270"
     CDS             complement(<1..285)
                     /locus_tag="PK742_11270"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77213.1"
                     /translation="MVIGILGEKADNRVAITPEHIQQLSGLKAEKILVESGAGNNAGY
                     PDSLYQEKGAVVTDRASVLRDADMLLCIQPIGRQELDSLKGKVLVGGFNPL"
     gene            431..503
                     /locus_tag="PK742_11275"
     tRNA            431..503
                     /locus_tag="PK742_11275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:464..466,aa:Thr,seq:cgt)
     gene            531..2969
                     /locus_tag="PK742_11280"
     CDS             531..2969
                     /locus_tag="PK742_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HQU77214.1"
                     /translation="MEYNKLLKAWYERQEWSAFPFQESLAQAYAEGLHGLLNAPTGSG
                     KTYAMFLPALCYSISQESNRKKAGHLRIIWITPLRALARDIMNALQHACDTMESGWQV
                     QMRTGDTDAKTKQAQKKKMPEVLIITPESLHVLLAAKGYPALFSSLEAVVVDEWHELL
                     GSKRGVQVELALSRLRALSPAIRTWGISATIGNLVEAMEVLVGTQMPPDKTTVVRSDI
                     KKEIAVHTVLPEKMDLLPWAGHIGLSLLPRIIDIIWSARTVLIFTNTRAMAEIWYHQI
                     LQAEEELAGQLAMHHGSLSREVRDWVESSLHAGTLKAVIATSSLDLGVDFRPVDTVIQ
                     IGSPKGVARFTQRAGRSGHQPGAVSNIYFIPTHALELVEVAALKEAIGSGIVERREPF
                     VQSFDVLVQYLVTLSIGEGFREKEILREVKATHAFHEMTRDEWEWCLAFITHGGKAFE
                     AYDDFNRVEVQDGRYRIANRTASIRHRLQIGTIVSDPVVRVSYLKGGTLGTIEEYFIA
                     RLKPGDVFWFAGLCLEFVMLKDMTALVRRTKEQKAGTASYQGGRMPLSGYLAEVMRKQ
                     LHEAEELAGTYEEYAMLAPLLDLQRNRTAIPAPDELLVEYSDSEDGVHLFFYPFEGRL
                     MHEVMGSLAAFRLSRELPVSISLAMNDYGFELLCNREIELTEDLVRRLLDPQKMDEDV
                     FGSVNAMEMARRKFRDIAVISGMVFQGYPGKIKKNRHLQSSSQLMFDVLTQYDPGHLL
                     LRQSYEEVINDQMDQARLRKALIRIHNGKVLIRKTSRFSPFSFPIVVDSMREKMSSER
                     MADRIRRMVEENGG"
     gene            3025..3183
                     /locus_tag="PK742_11285"
     CDS             3025..3183
                     /locus_tag="PK742_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77215.1"
                     /translation="MEQAKKKNKLMWWALGFLSLFACIYMIMFFSNFFWITLPLLFTS
                     FAMAMDGL"
     gene            3250..>3567
                     /locus_tag="PK742_11290"
     CDS             3250..>3567
                     /locus_tag="PK742_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77216.1"
                     /translation="MKGISLLCGAALLLSVSCQPSDSNKTENKIMRDPHSFSRPDQVS
                     VSHLDWLAYVDFGTTTLTANATWTLDKHSDRASQVIFDINGLHITSVVDQDGDTLEYS
                     LGIA"
BASE COUNT          868 a          889 c          973 g          837 t
ORIGIN      
        1 cagggggttg aaaccaccca ccagtacctt tcctttcaag gaatccagtt cctgtcttcc
       61 gatcggctgg atgcacagca acatgtcagc gtctctcagc acggaagccc ggtcagttac
      121 aactgcacct ttttcctgat acagtgaatc gggatatccg gcattatttc cggcacctga
      181 ttccaccagg atcttttcgg ctttcagtcc gctcagctgc tgaatatgtt caggggtaat
      241 ggcgacccgg ttgtcggcct tctcccccag aatgcctatt accataggta gaggattttt
      301 aatgtagcgc aaatatagct gaaaaaagga gaggcaggaa cagactttta tcattggaat
      361 aactgctgtg aaccgttgtc atgcagccgt tttcctttaa tcggcaggtc gttctacatt
      421 tgtgacaaca gcctccttag ctcagttggt agagcagttc attcgtaatg aacgggtcgt
      481 cggttcgagt ccgatgggag gctctttctc ttccgtcacc ctatcggcat atggagtaca
      541 acaaactgtt gaaagcctgg tatgaacgtc aggagtggtc ggcttttccc ttccaggaat
      601 cgctggcaca ggcctatgcc gaaggtctgc acgggttact caacgcaccc accggcagcg
      661 gaaagaccta tgccatgttc ctgcctgcgc tctgttattc cataagtcag gaaagcaacc
      721 ggaagaaggc cgggcatctc aggatcatct ggatcacccc gctcagggcg ctggcccgcg
      781 acatcatgaa cgcactgcag cacgcctgcg atacgatgga gtccggatgg caggtacaga
      841 tgcgtaccgg agatacggat gccaaaacca aacaggcaca gaaaaagaaa atgccggagg
      901 tacttatcat cactccggaa agtttacatg tgctgctggc agccaaaggt tacccggcac
      961 tgttcagctc tttggaagca gtagtggtgg atgaatggca tgaactgctg gggtccaaac
     1021 gcggggtgca ggtggagctg gccctgtcca ggttaagagc cttgtcgcca gccattcgta
     1081 cctggggtat atctgctacc atcggcaacc tggtggaagc aatggaagta ctggtaggta
     1141 cacaaatgcc tccggataag accactgtgg tgcgcagtga tatcaaaaag gagatcgctg
     1201 ttcacacggt attgcccgaa aagatggacc tgctgccctg ggcgggtcat attggactga
     1261 gcctgcttcc ccggatcatt gatatcatat ggagcgccag aacggtgctc atcttcacca
     1321 atacacgagc catggctgag atctggtatc accagatact ccaggcagaa gaagagctgg
     1381 ccggtcagct ggccatgcac cacggttcac taagccgtga ggtacgtgac tgggtggagt
     1441 catcgctgca tgccggtacc ctgaaggcgg tcatcgctac atcgagcctg gatctgggtg
     1501 tggactttcg gccggtggat acggtcatcc agataggttc cccaaaaggc gtagcccgct
     1561 tcactcagcg agcaggaaga agcggtcacc aacctggtgc ggtttccaat atttatttca
     1621 ttcccaccca tgccctggaa ctggtggaag tagccgcatt aaaagaggcg attggatccg
     1681 gtatcgtaga aaggagagag ccctttgttc agagtttcga tgtgctggtg caatacctgg
     1741 tgacgctcag catcggcgaa gggttccggg aaaaagagat tttacgggag gtaaaagcca
     1801 cccatgcttt tcatgaaatg acccgtgatg aatgggagtg gtgcctggca ttcatcacac
     1861 acggcgggaa agctttcgag gcatacgatg acttcaatcg ggtggaggtg caggatggaa
     1921 ggtatcgcat tgccaacaga actgcctcca tcagacacag attgcagatc ggaaccattg
     1981 tcagcgaccc ggtagtgcgg gtcagctatc tgaaaggcgg caccctgggc accatagaag
     2041 aatatttcat cgcgagactc aaacccggcg atgtgttctg gtttgccgga ctatgcctgg
     2101 aattcgtcat gctgaaagac atgaccgcac tggtcagaag aacgaaggaa cagaaagcgg
     2161 gaaccgcaag ctaccagggc ggtagaatgc ccttgtcagg ctacctggcc gaggtgatgc
     2221 ggaaacaatt gcacgaagca gaagagttgg caggtaccta tgaggaatat gccatgctgg
     2281 ctcctttatt ggacctgcag cggaacagga cagccattcc cgctcccgat gaactattgg
     2341 tggagtacag tgacagcgaa gatggcgtgc atctgttctt ttatcccttc gagggacggt
     2401 tgatgcacga ggtcatgggc agtctggcgg cattccgtct gagccgggaa cttcccgtca
     2461 gcatctcgct ggccatgaat gactatggct ttgaattact gtgcaatcga gagatagaac
     2521 tgaccgaaga cctcgtacgc cggttgctgg atccgcagaa gatggacgaa gatgtgttcg
     2581 gatcggtcaa cgccatggaa atggccaggc ggaaattccg cgatattgcc gtcatcagcg
     2641 gcatggtctt ccagggatat ccgggcaaga tcaagaagaa ccgtcacctg caatcttcca
     2701 gccagctcat gttcgacgtg ctgacacagt atgatccggg gcatctgctg ctgaggcaat
     2761 cctacgagga ggtcatcaac gaccagatgg accaggccag acttcgcaag gctttgatac
     2821 ggatccacaa cgggaaggtc ctgatcagaa agactagccg tttttcgcct ttcagcttcc
     2881 cgatcgtggt ggacagcatg cgggaaaaaa tgagttctga gcgcatggct gaccgcatca
     2941 ggcgtatggt ggaagaaaat ggtggataag gagtttgccg gatggtgtta atcctgtact
     3001 tttgtggctc ttaagtcaat accaatggag caagcaaaga agaaaaacaa actgatgtgg
     3061 tgggcgctgg gctttttatc cctcttcgcc tgtatctaca tgatcatgtt cttcagcaac
     3121 tttttctgga tcaccctgcc attgctgttc accagcttcg ccatggcaat ggatggcttg
     3181 taagcagccg gaactccttg tatgacggcc ttcacagccg tctttttttc atttccctaa
     3241 tttcgtgcta tgaaaggcat ttctctcctc tgcggggcgg cactgctatt gtccgtttcc
     3301 tgccagccct cagattccaa taaaacagaa aataagatca tgcgagatcc acattccttt
     3361 tcacgtccgg atcaggtgtc cgtttcacac ctggattggc tggcttatgt tgactttggt
     3421 acaacgactt taaccgccaa cgccacctgg actcttgaca aacattcaga tcgtgcaagt
     3481 caggtgatct ttgatataaa tggactgcat attacctctg tggtggatca ggatggtgac
     3541 acgcttgaat acagtttagg cattgca
//
LOCUS       DAOMAX010000265         3560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5119_length_3560_cov_3.965763, whole genome shotgun sequence.
ACCESSION   DAOMAX010000265 DAOMAX010000000
VERSION     DAOMAX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5119_length_3560_cov_3.965763"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1811
                     /gene="glgP"
                     /locus_tag="PK742_11295"
     CDS             108..1811
                     /gene="glgP"
                     /locus_tag="PK742_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQU77217.1"
                     /translation="MIKAAEWQFPYETDPRYHKSVAYFSMEFAIAQALKIYSGGLGFL
                     AGSHMRSAYELRQPVFGIGMLWRFGYYDQIRDDNGFMAVQFQKKLYTFLHQTDIEFSI
                     PIDGEDVLVKVYYLPHDVFHSAPLFLMTTDFPENSDKSRAYSHRLYDNWPENRAAQQV
                     ILGIGGAKITEALGGVDIYHLNEGHGLPLAFHLFNTFKDIEQVRERLVFTTHTPEKAG
                     NEEQRFDFLERMGFFDGLPVDLVKATTHTDGDMLSYTPATLVFSKMANAVSQLHGEVS
                     NDMWSHISDRSPIIAITNAQQKKYWADREMEQAFQEGNQHEIRQIKREMKTELFEVVA
                     NQTGKIFRPDVLTIVWARRFASYKRANLILRNKQRFLDMINNPDMPVQVIWAGKPYPM
                     DHDSVTMFNDIRSFIQGRPNCAILVGYELMLSRQLKRGSDVWLNTPRRPHEASGTSGM
                     SAAFNGSVNVSINDGWIPEFARHGQNCFVTPTADHHALSIQEIDDFDHENIMDVLENE
                     VIPAYYNDPERWVKIMQQSMQDVYPFFDSDRMAQEYFDRLYLPAYNPVERNLAAMADR
                     Q"
     gene            complement(1812..2057)
                     /locus_tag="PK742_11300"
     CDS             complement(1812..2057)
                     /locus_tag="PK742_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77218.1"
                     /translation="MRMLGTIAHTAYQITVFGWAEKYIVKIEARGLEQAYKFPQEAYD
                     SWKDLESLFDEAMLEQVRQVFQLMASAMSASVARRST"
     gene            complement(2054..2512)
                     /locus_tag="PK742_11305"
     CDS             complement(2054..2512)
                     /locus_tag="PK742_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQU77219.1"
                     /translation="MSDSWRKKIFLEIRKKPGKQAEKVAFEAEKAVFEHTDCLGCARC
                     CKGYNAILEEEDVIRISAHLKLEPHQFAATYMMVDEDDEWCLNGSPCPFLQADNKCSI
                     YPVRPASCADYPHMGSPGLVNREELTLINADICPAVDEILLQIEKKLQDL"
     gene            2678..3064
                     /locus_tag="PK742_11310"
     CDS             2678..3064
                     /locus_tag="PK742_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77220.1"
                     /translation="MKKGMLFIAAIMLLFMTKADAVAQVQDNVVLTDMGNGLYEVAVY
                     YEDGRLQEKGYMTNGLSTGQWVRYSPEGTILAVASYNESGEKDGIWNIYSTSGELAFE
                     LVYENNVRTMARSFDTEGDMVSYRVQ"
     gene            3129..>3560
                     /locus_tag="PK742_11315"
     CDS             3129..>3560
                     /locus_tag="PK742_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77221.1"
                     /translation="MQKFLPVWLACMLNAAFVAAQEVPGYEVYLMWWGGNEVNGYLYS
                     EPWNISAHTGYDNQPSFSPDGSQVLFSSLRDGVQTDIYSYYVPNGPLKRIAGTPESEY
                     SPEWMPDSKWISTVRVEMDGTQRLWRIRSDGRKARPLYPEFH"
BASE COUNT          960 a          831 c          845 g          924 t
ORIGIN      
        1 ataggagcag gtacaccggt ttccgggcac cagggggctg tgtgaacaaa tggtgaaacg
       61 ctattcttgt gcgcttgttt atattcgcac cgcaaaaaaa agacaacatg atcaaagcag
      121 cagagtggca gtttccttat gaaacagacc ctcgttacca taaaagtgta gcctatttct
      181 ccatggaatt tgccatagcc caggcactaa agatctattc aggaggcctc ggtttcctgg
      241 ccggctcaca catgcgaagc gcctatgaac tcaggcaacc tgttttcggg atcggcatgc
      301 tttggcggtt tggttattat gaccagattc gtgatgacaa cggcttcatg gctgtgcagt
      361 tccagaaaaa actgtacacc ttcctgcatc agacagatat tgaattcagc attcctattg
      421 atggagaaga tgtactggtc aaggtatatt acctgccaca cgatgtgttc cattcagcac
      481 ctctgtttct gatgacaacc gattttcctg aaaacagcga caaatcgcgt gcatacagcc
      541 acaggctcta tgacaactgg ccggaaaacc gtgcggctca gcaggtcatt ctgggtattg
      601 gcggggcaaa gatcacagaa gcactgggcg gggtggacat ttaccacctc aacgaaggac
      661 acggactgcc actggccttt catttgttca atacattcaa ggatattgaa caggttcgcg
      721 aacgccttgt atttacaaca catactccgg agaaagccgg aaatgaagaa cagcgtttcg
      781 acttcctgga acgtatgggc ttctttgacg gattgccggt tgacctggtg aaagccacca
      841 cacataccga tggtgatatg ctgagttata cacctgctac actggtattc agcaaaatgg
      901 ccaatgcagt atctcagctg catggagagg tttccaatga tatgtggtct catatcagtg
      961 acagaagccc gatcatcgcc attaccaatg cacaacagaa aaagtactgg gcagatcgtg
     1021 aaatggaaca ggcctttcag gagggtaacc agcacgagat ccgccagatc aagcgggaaa
     1081 tgaagacgga gctttttgaa gtggtggcca atcagacagg aaagatcttc cggcctgacg
     1141 tactgaccat tgtatgggcc agaagatttg cctcctataa acgagccaat ctgattctca
     1201 gaaacaaaca gcgcttcctg gacatgatca acaatccgga tatgcccgtt caggtcatat
     1261 gggccggtaa gccctatccg atggatcatg attctgtgac catgttcaat gacatcaggt
     1321 cctttataca gggaagaccc aactgtgcca ttctggttgg ctatgagctg atgctttccc
     1381 gacaactgaa gcggggttct gatgtgtggc tgaatactcc cagaaggcca catgaagcat
     1441 caggaacttc aggcatgtct gctgcattca acggttctgt aaacgtatcg atcaatgatg
     1501 gctggattcc tgaatttgcc cgtcatggcc agaattgttt cgtgacccca acagctgatc
     1561 atcatgcact gtccattcag gagatcgatg attttgacca tgagaatatc atggacgtgc
     1621 tggaaaatga ggtgataccg gcttattaca acgatccgga acgctgggta aagatcatgc
     1681 agcaaagcat gcaggatgtg tatccgttct tcgacagtga ccggatggca caggagtatt
     1741 ttgaccgcct gtatcttcct gcttataacc cggtagagag aaatcttgct gctatggcag
     1801 accggcagtg atcaggttga tcgacgggcc acagatgcac tcatagcaga tgccataagc
     1861 tgaaagacct gtctcacctg ttccagcatg gcctcatcaa acaggctttc caggtctttc
     1921 caggaatcat atgcttcctg gggaaatttg tatgcctgtt ccagcccccg tgcctcgatc
     1981 tttacaatgt acttctcagc ccacccgaat accgtgatct gataggcggt atgggcgatg
     2041 gttcccaaca ttctcataag tcctgaagct ttttttctat ctgtagcaaa atctcatcaa
     2101 cagccgggca aatatcggca ttgataagtg tcagttcttc ccggttgacc aatcccgggc
     2161 ttcccatatg cggataatcg gcacaggatg ccggtctgac cgggtagatc gaacatttgt
     2221 tgtcagcctg cagaaaaggg caggggcttc cgttcagaca ccattcatca tcctcatcca
     2281 ccatcatata agtggcggca aactgatgcg gttctaactt caggtgtgcg gatatcctga
     2341 taacgtcttc ttcttccaga atggcattgt atcccttaca acaacgtgca caacccaggc
     2401 aatcggtgtg ctcaaaaact gccttttcag cctcaaaagc aactttttct gcctgtttac
     2461 cgggcttttt cctgatttca agaaatattt tttttctcca actgtcagac attacacatt
     2521 gatatacaat agtttaggaa ctaaattgag tgcttttgtt aattgcatgt gaagaaaaat
     2581 taccctaatg ttaactgaat attaagcaaa acggaacaaa ttcttaactt tgatcgtaca
     2641 atcagttaac aaggttgtac ctccaaatct aatgaaaatg aaaaaaggaa tgctcttcat
     2701 cgctgcgatc atgttgcttt ttatgacaaa agcagatgct gtagcgcagg tgcaggacaa
     2761 cgtggttctg accgatatgg gtaatggtct ctatgaagtg gccgtttact atgaagatgg
     2821 tcgattgcag gaaaaggggt acatgacgaa tggtctgagc acaggacagt gggtgagata
     2881 cagccctgaa ggtactattc tggctgttgc cagttataac gaatccggag aaaaagacgg
     2941 tatctggaat atctactcca cctcaggtga actggcattt gaactcgtat atgagaataa
     3001 cgttcgcacc atggcccgca gcttcgacac agaaggagac atggtaagct accgtgtaca
     3061 ataatatttg cgctgttaat gtaaaagcct gcttcccccg gaagcgggct ttttttacat
     3121 ttgcctgtat gcaaaagttc ctgcctgtat ggcttgcctg tatgttgaat gccgctttcg
     3181 tggcagctca ggaagtaccc ggatatgaag tttatctgat gtggtgggga ggcaatgaag
     3241 taaacggcta tctctattct gaaccctgga atatttctgc tcatactggt tatgataatc
     3301 agccttcctt ttcgcccgat ggtagtcagg ttctcttcag ctccttgcga gacggagtac
     3361 aaacggatat ctattcatat tatgtaccca acggtccgct gaaacgtata gccggtacac
     3421 ccgaaagcga atactctccg gaatggatgc cagacagtaa atggatcagt actgtgcggg
     3481 tagaaatgga cggcacccag cgcttatggc gtatccgatc agacggcaga aaagcccgtc
     3541 ctctctatcc tgaatttcat
//
LOCUS       DAOMAX010000266         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5160_length_3541_cov_3.650889, whole genome shotgun sequence.
ACCESSION   DAOMAX010000266 DAOMAX010000000
VERSION     DAOMAX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5160_length_3541_cov_3.650889"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /gene="argS"
                     /locus_tag="PK742_11320"
     CDS             complement(<1..1311)
                     /gene="argS"
                     /locus_tag="PK742_11320"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQU77222.1"
                     /translation="MNHLSGLLQALQEAAQALYSYELNEEQCKLSPTPSEFEGEYTFV
                     VFPLLKASRKNPVETGNEIGHYLLEHCADIAAFNVVKGFLNLSLKDSFWTDLLRNIQD
                     EKAWNNLPSTGKKIVVEYCGPNTNKPLHLGHIRNMLLGMSTVRLLNAAGNDVHPVNIY
                     NDRGIAICKSMVAYVRSGQKTTPESTGTKGDHFVGDYYVEYAKQFDAEVKELMADGMA
                     KDEAEKKAPIYTEAVDMLQRWEAGDADTLALWNTMNSWVYAGFNETFQRLGVHFEKDY
                     HESVYFTKGRDLVLEGLEKGLFFRKDDGSVWVDLTDAGLDQKVLLRSDGTSVYITQDI
                     GVAHARYEDYHMDVSAYVVANEQDYHFKVLKLVLEKLEEPYAEGIFHLSYGMVDLPEG
                     KMKSREGTVVDADDLMDEMEKSSREFLEQSDKQQDFTAEEKETLY"
     gene            complement(1308..2261)
                     /locus_tag="PK742_11325"
     CDS             complement(1308..2261)
                     /locus_tag="PK742_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HQU77223.1"
                     /translation="MSAVRVISVNSELGAGTRGSGLGFDAIRVAAWTKGSRYFKNHPP
                     TIIPSNNDEVLDDIETAYAVKIHYIVEMYKRIAETVVDTLKAGNFPILISGDHSNAGG
                     TIAGLRMAYPEEKLGVIWIDAHADIHSPYTTPSGNLHGMPLAASTGLDNKVCQINQPE
                     ENTIAAWEELKNIGGIQPKVGFKDLIFIAVRDLEKAERFILKQNKVKNYTVQKMRETG
                     TSFVVEDALKRLEHCDRIYVSFDVDSMDANISRGTGTPVKSGLYESEAFQLMSAFAKH
                     PKVCCMELTEINPTLDNKKNLMAEIALEILEKVTLEIENRL"
     gene            2337..>3541
                     /locus_tag="PK742_11330"
     CDS             2337..>3541
                     /locus_tag="PK742_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQU77224.1"
                     /translation="MKNTYLDLIQQTFYFPQEGFDVKNNWLHFNGIPLKQLIEEYGTP
                     MRITYLPKISQQIQKAKKLFSNAMDELDYKGKYYYCYCTKSSHFAHVLDEALKNDIHL
                     ETSSAYDIDIIEKLYLNKRITKNTFIVCNGFKTNVYTQRISKLANLGFNIIPVLDNKE
                     ELKEYEDRIINDCQIGIRIAAEEQPEFQLYTSRLGIRYSEIRSFYEQYVQTNPKFKLR
                     MLHFFINSGIRDDIYYWNELTKSLKVYCEMRKICEDLTALNIGGGFPVRNSLGFEYDY
                     TYMVREIINKVQTTCEQEGVPCPDIFTEFGSFTVAESGAHIFSVLAEKQQNDSERWYM
                     IDNSLMTTMPDAWGINSRFILLPVNKWSGEVQRVNVGGLSCDQMDYYNSEAHTNEVYL
                     PRMDNTSPL"
BASE COUNT          814 a          920 c          875 g          932 t
ORIGIN      
        1 gtagagtgtc tctttttctt ctgcagtgaa gtcctgttgt ttgtcgcttt gctccaggaa
       61 ctcgcggctg ctcttctcca tttcgtccat caggtcatcg gcatccacaa ccgtaccttc
      121 ccggctcttc atcttgcctt ccggcaggtc caccatacca tagctgaggt ggaagatccc
      181 ttcggcataa ggttcttcca gtttctccag cacgagcttg agtactttga aatggtaatc
      241 ctgttcgttt gccaccacat aggcacttac gtccatgtgg tagtcttcgt aacgggcatg
      301 cgccacgcct atatcctggg tgatgtacac agaagtacca tcactgcgca gcaatacttt
      361 ctggtccagt ccggcatcgg tcaggtccac ccaaacagag ccatcgtcct tcctgaagaa
      421 gagtcccttc tccagtcctt ccagcacgag gtcgcggcct ttggtgaaat aaacggactc
      481 atgatagtcc ttctcaaaat gtacgcccag gcgttgaaag gtctcattga aacctgcata
      541 cacccagctg ttcatggtgt tccatagtgc cagggtatcg gcatcaccgg cttcccatcg
      601 ctgcagcatg tccactgctt ctgtatagat gggtgctttc ttctcagctt cgtctttggc
      661 catcccatct gccatcagtt ccttcacttc tgcatcgaac tgcttggcgt actccacgta
      721 atagtcgcct acaaagtgat cgccttttgt tccggtgctt tcgggtgtgg tcttctgtcc
      781 gcttcgtaca taagccacca tactcttgca gatggcgatg ccccggtcgt tgtagatatt
      841 gaccggatgc acatcattcc ccgcagcgtt gagcaatcgc acggtgctca tgcccagcaa
      901 catattgcgg atatgaccca ggtgcagcgg cttgttggtg tttggtccgc agtattccac
      961 cacgatcttt ttaccggtgg atggcaggtt gttccaggct ttttcgtcct ggatattccg
     1021 gagcagatcc gtccagaaac tgtctttcag cgacaggttc agaaagcctt tcaccacgtt
     1081 gaaggcggcg atgtctgcac aatgttccaa caggtagtgg ccgatctcat tccctgtttc
     1141 taccggattc ttccggcttg ctttcagcag cggaaagacc acaaaggtgt attcgccttc
     1201 aaactcagaa ggagtaggag agagcttgca ctgctcttca ttcagttcat agctgtacag
     1261 tgcctgcgcc gcttcctgca gcgcctgcag caatccgctc aggtgattca tagtcggttc
     1321 tcaatttcca gggtcacctt ctcgaggatc tcgagggcga tctctgccat caggttcttc
     1381 ttgttgtcga gggtgggatt gatctcggtc agctccatgc agcatacttt gggatgcttg
     1441 gcaaaagcac tcatcagctg gaaggcctcg ctttcataca gaccgctctt gaccggtgta
     1501 ccggtgccgc gactgatgtt cgcatccata ctgtccacgt caaagctgac gtaaatgcga
     1561 tcgcagtgtt cgagtctttt cagggcatct tccaccacga aacttgtgcc tgtctcccgc
     1621 atcttctgca ctgtatagtt cttgaccttg ttttgtttca ggatgaagcg ttctgctttc
     1681 tccaggtcac gcacggcgat gaagatgaga tccttgaatc cgaccttggg ctgaatacct
     1741 ccgatgttct tcagctcttc ccaggcggcg atggtattct cttcaggctg gttgatttga
     1801 cagaccttgt tgtccagacc ggtactggca gccagcggca taccatgtaa gttcccggac
     1861 ggtgtagtgt aaggcgaatg gatatcagca tgtgcatcga tccagatgac accgagtttt
     1921 tcttcgggat aggccatccg caaaccggcg atggtacctc cggcattgct gtgatcgccg
     1981 gaaatgagga tggggaagtt tccggctttc agggtgtcca ccacggtctc ggcaatgcgc
     2041 ttgtacatct ccacgatgta gtggatcttg accgcatagg cggtttcgat gtcgtccagt
     2101 acctcatcgt tgttgctggg tatgatggtg ggcggatggt tcttgaaata gcggctgcct
     2161 ttggtccagg ctgccaccct gatagcatca aaacccagac cggaacctct ggttcccgca
     2221 cctaattcgc tgttgacgga gatgactcgt actgcactca taaaggttca aagttattac
     2281 ttttgtcccg catgctgcac catggttgaa ccgacagaca aaccccatca ggaaccttga
     2341 aaaataccta cttagacctc attcagcaaa ccttctactt tccgcaggag ggctttgatg
     2401 taaagaacaa ttggctgcat ttcaacggaa tcccgctgaa gcagcttatt gaggaatatg
     2461 gtacgcctat gcgaatcact tacctgccca agatctccca gcagatccaa aaggccaaga
     2521 agttgttcag caatgcgatg gatgagctgg actacaaggg gaagtactat tactgttatt
     2581 gtaccaagag ttctcatttt gcgcatgtgc tggacgaggc gctgaaaaat gatatccatc
     2641 tggagacctc ttcggcttat gatattgata tcattgagaa gctgtatctg aacaagcgga
     2701 tcaccaagaa tactttcatt gtgtgcaacg ggttcaagac gaacgtttat acacaacgca
     2761 tcagcaagct cgccaacctg ggctttaaca tcattccggt gctggataat aaagaagagc
     2821 tgaaagagta tgaagaccgc atcatcaatg attgtcagat cgggatacgg atcgcagcag
     2881 aagaacagcc ggagtttcag ctctatacca gcagactggg catccgctat tcagagatca
     2941 gaagtttcta tgagcagtac gtgcagacca accccaagtt caagctccgg atgctgcact
     3001 tcttcattaa cagtggtatc cgcgatgaca tctactactg gaacgagctg accaagtcgc
     3061 tgaaggtgta ttgtgagatg cggaagatat gcgaagacct gaccgccctg aacatcggtg
     3121 gtggttttcc ggtgcgcaac agcctgggct ttgagtatga ctatacctat atggttagag
     3181 agatcatcaa taaagtacag acaacgtgtg agcaggaagg agttccttgt ccggatatct
     3241 tcacggagtt cggcagtttc acggtagctg aaagtggtgc acatatcttt tctgtactgg
     3301 cagaaaaaca gcagaacgac agcgagcgct ggtacatgat agacaactcc ttaatgacca
     3361 ccatgcccga cgcatgggga atcaattccc gtttcatcct gctgccggtc aacaagtgga
     3421 gtggggaagt acaaagggtg aacgtgggcg gattatcctg cgaccagatg gattactaca
     3481 actctgaggc acataccaac gaagtatatc tgcccaggat ggataatacc agtccgctgt
     3541 a
//
LOCUS       DAOMAX010000267         3524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5203_length_3524_cov_3.747189, whole genome shotgun sequence.
ACCESSION   DAOMAX010000267 DAOMAX010000000
VERSION     DAOMAX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_3524_cov_3.747189"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK742_11335"
     CDS             <1..322
                     /locus_tag="PK742_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HQU77225.1"
                     /translation="EVAVYAQLDGLIISNTTINRSGLLTKKLEVDRMGPGGLSGKPVF
                     ERSNEVLSFMRELLPETMDIIGVGGVFTKEDMEKKQALGAKLVQVYTGFIYTGPLMVK
                     QLVD"
     gene            complement(623..1588)
                     /locus_tag="PK742_11340"
     CDS             complement(623..1588)
                     /locus_tag="PK742_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HQU77226.1"
                     /translation="MTSRRQFIRRTSALGVGLLAFSRLSKAASPTFDGPVVLSTWNFG
                     LQTNEAAWKILAEKGRAVDAAEAGARLAEDDLKSTSVGLAGRPDREGITTLDACIMDE
                     FYNCGSVCFLEKIKHPISVARKVMETTPHVMLAGSGAQQFALENGFELDDYVNPESEK
                     AWHEWLKTSEYKPIINFENHDTIGIVALDAHGNLGGACSTSGMAYKIRGRVGDSPIIG
                     AGLYVDNEVGACTATGTGEEVIRIAGSHLVVEKMRDGMSPEDACRYAVERMIRIRPEQ
                     SKTLQVGFIAVNKSGEYGGYCIQPGFQYAVHTNEGNVLVDARSYY"
     gene            1676..3493
                     /locus_tag="PK742_11345"
     CDS             1676..3493
                     /locus_tag="PK742_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77227.1"
                     /translation="MKALLTFVFVLGAFALSAQSLVPNGDFESSPACPPFFSGIEFDS
                     NPYVDNWLRPTGATSDYFHECADAFSSVSIPTNYFFQYQYAHSGVAYGGFYVSVYGSS
                     YREYLQVQLAEPMLAEQCYYVEFYTAPAEVNDGTFAAVAIDELGLYISVDRLASDDIY
                     YYDYLDVDPQIKSPDGYFLSDTLNWIKISGIYVAEGGEEWITIGNFQSSADTETEEII
                     SGPGYDLSYYAIDDVSITALTGISVLPDSILCVGESLLLESVEGADSYTWNTGETTEA
                     ITVTESGTYVVTLEFECGVFNDTAEIIFINDGISYSNIDLVACYDELPLILEGSDLYI
                     SYNWSTGETTTDISVSESGTYVLTAYGGCATFIDSFLVDVIAEIPEADLPTDILICEV
                     NGSIELSGPDGMDFYSWSTGENTQFITVSDAGDYNLDYGKDCDAFTHVFHVFTDPFLE
                     WTLDVPDELTLCPYGSPVVFIQATSGFPSYNWSTGATSDAITVAESGTYTVNVQTLCL
                     DLTDSVRVVDCEVMEVPNAFSPNNDGYNDLFSVLCNPCDGFRMLAVYNRWGETVFETT
                     DPNTGWDGMIDGTQAPVGAYAWVLQYATGTKQGQVILIR"
BASE COUNT          865 a          835 c          865 g          959 t
ORIGIN      
        1 agaggtggcg gtctatgcgc aactggacgg actgatcatc agcaatacca cgatcaaccg
       61 aagcggcctg ctcacaaaga agctggaagt ggacagaatg gggccaggcg gattaagcgg
      121 aaaacctgtg tttgaacgct ccaacgaagt gctctctttt atgcgggaat tgcttcccga
      181 aacgatggac atcatcggag taggaggggt cttcaccaaa gaagatatgg agaagaagca
      241 ggcactcggc gccaaactgg tgcaggtgta caccggattc atttataccg gtccgttgat
      301 ggtgaagcaa cttgttgact aaaaggcgaa ggtcggagta tagaaaagtt ggcagtttgc
      361 tcctgaagga gctggcagtt ggcagttggc aaataaaagg tattccgtcc ccacagagtt
      421 acctgcatgc ccgccggagg agggtaagga attctgtggc tttatgttcc aattccagcc
      481 atgacgaaat ataaataagt tggcagttca cgtatcatca ggagcacctc accattcact
      541 gctctactca tacactgctg ccgcaggcaa ctactcaata cccaatactc actactcact
      601 actcactact caatactcaa tactaataat acgatctcgc atccaccagc acattccctt
      661 cattggtatg aacagcatat tgaaatcccg gctgaatgca atacccgccg tactcacctg
      721 acttgttgac ggcaataaaa cctacctgca gtgtttttga ctgttccggt ctgatgcgta
      781 tcatgcgttc cacggcataa cgacaggcat cttcgggcga catgccgtcc cgcattttct
      841 ccactaccag gtgtgagcct gcaatacgga tcacttcttc tccagtaccg gtagcggtgc
      901 aggcacccac ttcattatcg acatacaatc cggctccgat gataggggaa tcacccaccc
      961 gtccgcggat cttgtatgcc attccgctcg tgctgcaggc gccacccaga ttgccatgtg
     1021 cgtctaacgc cacgattccg atggtgtcgt ggttctcaaa gttgatgatg ggtttatact
     1081 ccgatgtctt cagccattca tgccaggcct tttctgattc agggttcacg tagtcgtcca
     1141 gttcaaagcc gttctccaaa gcaaactgtt gtgccccgct gccggccagc atgacatgcg
     1201 gagtggtttc catcaccttt cttgctaccg atatggggtg ctttatcttt tccaggaagc
     1261 ataccgatcc acagttgtag aattcatcca tgatacaggc gtcgagggta gtgatgcctt
     1321 ctctgtctgg tctgccagcc agtcctacag aggtgctttt gagatcatct tcagcaagcc
     1381 ttgcaccggc ctcagcggca tccactgcac ggcctttttc ggccaggatc ttccaggcag
     1441 cttcgttggt ttgcaagcca aaattccagg tggacagtac tacagggccg tcaaatgtag
     1501 gtgaagcagc cttgcttaat ctggagaagg cgaggagccc tacacctaag gcggaggttc
     1561 tgcggatgaa ttgtctgcgt gatgtcatat gctttgcaat ctgcagatga aagtaacgga
     1621 taatattcag atgtcttcgg aatatgcaga ttcaaaaatg taatttagat aattgatgaa
     1681 agcactcctc acattcgttt tcgtcctggg agcttttgcg ctgtcagccc aatctctggt
     1741 gccaaacggc gattttgagt catcccccgc atgcccgccg ttcttcagcg gaatagaatt
     1801 cgacagtaat ccttatgtag ataactggct tcgacctaca ggtgccactt cagactattt
     1861 ccatgaatgt gctgatgcat tcagcagtgt gagtatcccg accaattact tttttcagta
     1921 tcagtatgcc catagtggtg tagcctatgg cggcttttat gtatctgtat atggttcttc
     1981 ttacagagaa tatctgcagg ttcagcttgc tgaacctatg cttgcagagc aatgctatta
     2041 tgtggagttt tatactgcac ctgcagaggt gaacgatggc acatttgctg cggtagcaat
     2101 agatgagctc ggactgtata tttcagttga tcggctcgca tctgatgaca tctattatta
     2161 tgactacctg gacgttgatc ctcagataaa gagcccggat ggttactttc tgtctgatac
     2221 gctgaattgg atcaaaattt ctgggatata tgtggctgag ggtggcgaag aatggataac
     2281 gataggaaat tttcagtcat cagctgatac tgaaactgag gagatcattt ctggtcccgg
     2341 atatgacctg agttattatg ccatcgatga tgtaagtata accgctttga caggtatttc
     2401 tgtgttgccc gacagtattc tatgcgttgg tgaatcgctg ttgctggaat ctgttgaagg
     2461 agcagactca tacacctgga acaccggaga aactactgaa gcaattactg ttaccgaaag
     2521 tggtacttat gtggtcactc tggaatttga atgtggcgtg ttcaatgata ctgctgagat
     2581 catatttatc aatgacggga tcagctattc aaatatagac ctggttgcct gttatgatga
     2641 gctcccactg atcctggaag gttcagatct ctatatcagt tataactgga gtaccggtga
     2701 aaccaccact gatatctcag tttctgaatc aggtacctat gtgcttaccg cttatggtgg
     2761 atgcgccact tttattgaca gcttcctggt ggatgtcatc gcagaaattc ctgaagctga
     2821 tctcccaacg gatatcctga tctgtgaagt gaacggaagc attgaattat ctggtcccga
     2881 tgggatggac ttttattcct ggagtaccgg agagaatact cagtttatca ccgtgtctga
     2941 tgcgggagac tacaaccttg actatggaaa ggattgcgat gccttcaccc atgtgttcca
     3001 tgtctttacc gatccgttcc tcgaatggac tctggatgtt cccgatgagc tgaccctttg
     3061 cccgtatgga tctcctgtgg ttttcataca agccacgtcc ggatttccat cgtataactg
     3121 gagcaccggt gctaccagcg acgccatcac ggttgccgag tcaggcacct atacggtcaa
     3181 tgtgcagacc ttgtgtctgg acctgacgga ttcagttcgt gtggtggatt gtgaggtgat
     3241 ggaagtaccc aacgctttca gtccgaacaa cgatggctac aatgacctgt tcagtgtctt
     3301 gtgtaatccc tgcgatggat tcagaatgct ggcggtgtat aacagatggg gagaaacggt
     3361 ttttgaaacc acggatccaa ataccggttg ggatggaatg atagacggaa cccaggctcc
     3421 cgtaggtgca tatgcctggg tattgcaata tgccaccggc accaaacaag gacaagtaat
     3481 tcttatccga tgatcattgc gtctgccagg ttctgctgta attc
//
LOCUS       DAOMAX010000268         3523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5209_length_3523_cov_4.433391, whole genome shotgun sequence.
ACCESSION   DAOMAX010000268 DAOMAX010000000
VERSION     DAOMAX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5209_length_3523_cov_4.433391"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK742_11350"
     CDS             <1..551
                     /locus_tag="PK742_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQU77228.1"
                     /translation="IVNSDDPGIVYHSLGGNGSSFRAFARCDLFASQLAYLQPDLVII
                     SIGTNDTSDPDFDPEAYTAAYALFIEQILLVNPHCALILTVPNDSYVRKKYHNNNLVS
                     CRESIYTLAAKYHAAVWDFYTIMGGAYSAKKWRTDQIMKSDLIHFTNEGYQLKGALLM
                     EAMMEDYHHWLVDSGKERETGK"
     gene            551..2068
                     /locus_tag="PK742_11355"
     CDS             551..2068
                     /locus_tag="PK742_11355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQU77229.1"
                     /translation="MERLSRIFAFDVQHPMVFTRVDFWIFFLVVYAGITLLRNRLSWR
                     NAYLMAVSFFFYYKTSGLFLLLLFFSVSVSYWMSEWIYRSDSKTKKKVLLYLTITLQL
                     FLLVYFKYAYFFAGIASDFLGREVPVMNHFAWIANNILQFNWFDVDTILLPVGISFFT
                     FQVITYVVDVSKGKLDPVRSFTDYAFYISFFPQLVAGPIVRAADFIPQLYNKSSISKE
                     AFGTALFLILKGLFKKIFIGDYLAVNFIDRVFAHPLSYTGLENVFALISYSLQVYVDF
                     SGYTDIAIGLSLLMGFQLAKNFNSPYKAQNVGDFWRRWHISLSTFLRDYLYIPLGGNR
                     GTAFRTNVNLMITMLLGGLWHGASWNFVIWGGLNGLGLLIYKFWKKIRPWKKIEKAWA
                     MAGRIAFTFAFITFTRIWFRAPDLEGVQAFFQAVGTNFNWAVLPDFIWGFRLVMLVML
                     LGFVTHWLSDAFKQKWQQRFVDSPLWLQGVITVVMVFIIYQSVSAGMQPFIYFQF"
     gene            complement(2072..>3523)
                     /locus_tag="PK742_11360"
     CDS             complement(2072..>3523)
                     /locus_tag="PK742_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77230.1"
                     /translation="AQVLNENRIEFDLNEDYEDFGLSAYGADGLILYAKLEKKDIWKL
                     ERYSTDLKSDKSVNVTIGKKQTLKKKVEDENELNMLFTDAKGNYTFIHVEPATLEVTK
                     KSGVMPKKTQVGEIAILNNLVLVAGQNTKGPVLFTIDYQSGKQTFIPLAISGYGPKDL
                     TIENMQIMEDETEAIIYINAADSRLGKNVYDQSVYAMHINTSSKKTDLYMIETDGEHQ
                     LTSVSASKTGDDEYILTGTYSTRGTNTSQGIFIAGVSNKTVSFIKYHNFLDFNEFLSY
                     LPDRKKEKIEKKKERKEDNDKEFSIDYLMASHEIAVMNDQYIYLGEAYYPTYRTETYT
                     TTVNGQTVTRTRTVFDGNQYTHATIAAFDDEGELIWDRTFEMWPSYKPFFAKRFIELN
                     IDEDNINMLFASNNSIKSMQVDDNGKILHDNSAELIDTGSDSDKVKDSYSNLSYWYDN
                     YFLAHGNQTIKDSDASFGKKKRRVYFINKISFE"
BASE COUNT          855 a          774 c          797 g         1097 t
ORIGIN      
        1 gtatcgtcaa tagtgatgac cctggcattg tttaccattc acttggtggg aatgggagca
       61 gttttcgggc ctttgccaga tgtgatctgt ttgcttccca actggcctac ctgcaaccag
      121 atcttgttat tatctctatt ggcacaaatg ataccagcga tcctgacttt gatcctgaag
      181 cttacactgc agcatacgca ttatttattg aacagatact gttggtcaat ccgcactgtg
      241 cactgatcct gactgtaccc aatgacagct acgtgcgtaa gaaatatcac aacaacaacc
      301 tggtttcctg ccgggaaagc atctatacac tggccgcaaa ataccatgca gcagtctggg
      361 atttttatac catcatgggt ggcgcataca gtgctaaaaa atggcgtacc gatcagatca
      421 tgaagagtga tctgatccac ttcaccaatg aaggctatca gctcaaagga gcactgctca
      481 tggaagccat gatggaagac taccaccatt ggctggtcga ttcaggtaaa gaaagagaga
      541 ccggaaagta atggaaaggc tgagccggat attcgcattt gatgtgcagc accctatggt
      601 gtttacccgg gtagatttct ggatattctt tctggtggta tatgctggaa ttactttgct
      661 gcgaaacagg ctgagctggc ggaacgccta cctgatggca gtcagttttt tcttttacta
      721 taagaccagt gggctttttc tcctgctttt gtttttttct gtttctgtca gttactggat
      781 gtcagaatgg atttaccgaa gtgacagtaa aacgaagaaa aaggtattgc tctacctgac
      841 cattaccctg cagctttttc tgcttgtgta ttttaaatat gcctatttct ttgccggcat
      901 tgcaagcgat tttttaggca gagaggtacc ggtcatgaat cattttgcat ggatagcgaa
      961 caacatcctg cagttcaact ggtttgatgt agataccatc ctgctgccgg taggtatcag
     1021 tttcttcacc tttcaggtga tcacctatgt ggtggatgta tccaaaggga aactggatcc
     1081 tgtaaggtcc ttcactgatt atgcgttcta tatttccttc tttccccaat tggtagcagg
     1141 acctattgtg cgggctgcag attttatccc gcaattgtac aataagagca gcatttcaaa
     1201 agaggcattc ggaactgcac ttttcctgat cctgaaaggg ttgttcaaaa agatattcat
     1261 tggcgattac ctcgcggtca attttattga cagggtcttt gcccatccgc tcagttatac
     1321 cggacttgaa aatgtatttg cactgatcag ttacagtctg caggtctatg tggatttttc
     1381 aggatatacg gatatcgcca ttggcttgtc actgctgatg ggtttccagt tggcaaagaa
     1441 tttcaactct ccctataaag cacaaaatgt gggtgatttc tggcggcgat ggcatatcag
     1501 tttgtccacc tttctccgcg actatctcta tataccgctg ggaggaaaca ggggtacagc
     1561 tttccgcacg aatgtgaacc tgatgatcac catgctgctg ggaggtctgt ggcatggtgc
     1621 cagctggaac tttgtgatct ggggcggact gaacggactg ggtctgttga tctacaagtt
     1681 ctggaaaaag atacgcccct ggaagaagat cgaaaaagca tgggccatgg caggacgcat
     1741 cgcttttaca tttgccttta tcacctttac caggatctgg ttcagggctc cagacctgga
     1801 aggagtacag gcattctttc aggccgtggg caccaatttc aattgggccg tactgcccga
     1861 tttcatctgg ggtttccgtc tggttatgct ggtgatgctg ctgggctttg tgacccattg
     1921 gctgagcgat gcattcaagc agaaatggca gcagagattt gttgacagtc cgctgtggct
     1981 gcagggagtc atcaccgtag tgatggtctt catcatctac cagagtgtat cggccggcat
     2041 gcagcctttc atctacttcc agttctgagt atcactcaaa actgatcttg ttgatgaaat
     2101 agacacgtct cttcttttta ccgaatgagg catcagaatc cttgatcgtc tgatttccgt
     2161 gtgccagaaa ataattatcg taccaatagg acaagttaga atatgaatcc tttactttat
     2221 cgctgtcact tccggtatcg atcaactcag cactattgtc atgcaggatc tttccattat
     2281 catcaacttg catggatttg atagagttgt tgctggcaaa taacatattg atgttatcct
     2341 catcgatatt caattcaatg aaccttttag caaagaatgg tttgtaactg ggccacattt
     2401 caaaggttct gtcccagatg agttctcctt catcatcaaa tgcagcgatc gttgcatgtg
     2461 tatattgatt cccgtcaaaa acagtacggg tgcgggttac tgtttgtccg tttacggttg
     2521 ttgtataggt ctcagtgcga taagtagggt aatatgcttc ccccagatag atatactgat
     2581 cattcattac agcgatctca tgcgaggcca tcagataatc gattgaaaac tccttgtcgt
     2641 tatcttcctt cctttctttt ttcttttcga tcttttcctt cttcctgtcg ggcaggtagg
     2701 agagaaactc attgaagtcc agaaaattgt ggtatttgat aaagctgact gtcttatttg
     2761 agactcctgc aatgaatatc ccctgagagg tgtttgttcc tctggtactg taagtgcctg
     2821 tcagaatata ttcatcatct ccggtcttgg atgcagaaac ggaagtaagc tggtgttccc
     2881 catctgtttc gatcatatac aggtctgtct tcttgctgct tgtattgata tgcatggcat
     2941 agacagactg gtcataaaca tttttcccca gtctgctatc agctgcattg atataaataa
     3001 tggcttcagt ttcgtcttcc attatctgca tattctcgat cgtgagatcc tttggtccgt
     3061 aacccgagat ggcaagggga ataaaggttt gtttgccgct ttgatagtcg atcgtgaaca
     3121 atacagggcc tttggtattc tgcccggcaa ccagaaccag gttattgaga atggcgatct
     3181 caccaacctg ggtcttttta ggcatgaccc cgctcttttt ggtcacttcc agggtggccg
     3241 gctccacgtg aataaaggtg tagttacctt ttgcatcggt gaacagcata ttaagctcat
     3301 tttcatcctc gacctttttc ttgagggtct gctttttccc gatagttaca ttgacagact
     3361 tatcagactt gaggtcagta ctgtatcttt ccagtttcca gatatctttc ttttcgagtt
     3421 tggcatacag gatcaaaccg tctgcaccat atgcactaag cccgaaatct tcataatctt
     3481 cattcaggtc aaattcaatt cggttctcat tgagaacctg agc
//
LOCUS       DAOMAX010000269         3508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5244_length_3508_cov_2.345497, whole genome shotgun sequence.
ACCESSION   DAOMAX010000269 DAOMAX010000000
VERSION     DAOMAX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_3508_cov_2.345497"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1569)
                     /locus_tag="PK742_11365"
     CDS             complement(52..1569)
                     /locus_tag="PK742_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77231.1"
                     /translation="MRRNIFLMLILSFPLISFSQWTQKGSDISGLEEGEYCGYAVSLN
                     ANGQVAAVGSPYYSIPEDYTGLARIFEYSGSEWTQRGSNITGLETYSWGGMALSLSDD
                     GNVVAVPSRSANNSLGLPSGYVRIFEWDGSDWSQKGEIIEGEGNPLFFLDYFGYDVSL
                     SSDGNVIAIGGPYRWGTDSLGNSMVKSGHARVYAWSGTEWQQRGVDLEGSEASQNLGF
                     SVSLSSDGATLAVGSPQKSETEYAAGQVEVFMWDGNNWVSKGASINGILEGEKSGTSV
                     SLSGDGNTLAIGSPGYIPFHENDHSEVRIFDWDGNNWIQRGSDIIGPYEGDETGKSVS
                     MSQDGNTVVIGAPSSDDHINLIMNAGVIYIYRWDGSNWIQIGEDIYGEESLEFCGNTA
                     AISKDASTIIAGSFGYGENGRARIYSDESWVSINRPDDILFDLYPNPCSEYIVVVSPE
                     LSGTMEIVNIRGQKVFSGEILAGYNRFDIQTLTPGVYIANINSEHGNKSIELIRQ"
     gene            complement(1721..3250)
                     /locus_tag="PK742_11370"
     CDS             complement(1721..3250)
                     /locus_tag="PK742_11370"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU77232.1"
                     /translation="MQHVWEDITLSDSARFTAFATYYNAYIFSNPHSCIIVTKKHYDL
                     AKQLQNPKEMAEALNKKALAFYILSDADQSLTALGEASSILSSIGDAAGLARIQSNIG
                     NIYRQLDNYQEAIHIYVQSLFILNELDTLPESRADMLNNIGLIYQDVQMYPLALEYLE
                     KARNLYASIGLEEEVGNIWLNTGAVYLAMGNTETALLNYYKALPILKRKNNMLSSADC
                     MYELSHTYNILNKQDSALYYISLCLDISESLGNEERIISSSLLYAALIAGSNPQEAMA
                     LGEDIYRKIDGIPRLELRASTVRLLYESYKALGRPADALFMLERYQQYSDSLQLAEDQ
                     IGIIRQAIQSEFDQKLFVTQLENEKAQATLRLKQERRIYFILLVTLLLLITGSVYIRL
                     RSQKHRRQRDLLLAEIEQLKWNATASVTSSDAFLLNRQYIEAQLKRKLNETDWKVLNV
                     LLVNPVISNKDLSEEVHLSIDGIGSSLRRMYAIFEVPESRYKKIALLMQAVKLSGKPA
                     S"
     gene            complement(3404..3505)
                     /locus_tag="PK742_11375"
     CDS             complement(3404..3505)
                     /locus_tag="PK742_11375"
                     /inference="COORDINATES: protein motif:HMM:NF021483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HQU77233.1"
                     /translation="MEDPLMQKIRYMDKLVDELAKGKKMEKILREAN"
BASE COUNT          954 a          814 c          681 g         1059 t
ORIGIN      
        1 ttaaatttag gaaatgaaaa aattattaaa ttagaacaaa aagaaaatga tttattgcct
       61 gatcaattct atactcttgt tgccgtgttc actgttgatg ttggcgatgt aaactcccgg
      121 ggtaagagtt tgaatatcga atctgttgta accggcaaga atttcgcctg aaaatacttt
      181 ttgtcctcga atattaacga tttccatggt gcctgacaat tcgggagata caacaacaat
      241 gtattcgctg caaggattgg gatacaggtc aaacagtatg tcgtcgggtc ggttaatact
      301 aacccagctt tcatcgctat atatccttgc ccgaccattt tcgccatacc cgaatgaacc
      361 agctatgatg gtgctggcat ctttgctgat ggctgctgta ttgccacaga attctaagct
      421 ttcctctcca tatatgtctt cccctatttg tatccagttg ctgccatccc atctatagat
      481 ataaataact ccagcattca taataagatt gatgtgatca tctgatgaag gagcacctat
      541 tactacagta tttccgtcct gactcataga tacagatttg ccagtttcat ctccctcata
      601 gggtccaatg atatcacttc ccctctgaat ccagttgtta ccatcccagt caaaaattct
      661 aacttctgaa tgatcgttct catgaaaagg aatatagccg ggtgagccaa tagcaagtgt
      721 atttccatcg ccactaagac taacgctggt tccggatttc tcaccttcca gaataccatt
      781 tatgcttgcg ccttttgaaa cccagttgtt accatcccac ataaagactt ctacctggcc
      841 tgctgcatat tcagtttcgc ttttttgtgg tgaacctact gccaaagtag caccatcgga
      901 actcagcgac acggaaaagc caagattctg tgaggcttca gatccttcca gatcaacacc
      961 cctttgctgc cattctgtgc ccgaccatgc atacaccctt gcatgaccgg atttgaccat
     1021 tgaatttccc aaagaatcgg ttccccagcg atagggcccc ccaatggcga tcacattccc
     1081 atcgctgctc aggctaacat catatccaaa gtagtcaagg aaaaacaagg ggttgccttc
     1141 accttctatg atctcacctt tttgagacca atcgctgccg tcccactcaa atattctgac
     1201 atagccagat ggaagcccca aactattgtt agcggacctg gatggcactg ccaccacatt
     1261 accatcgtca ctcagactca acgccattcc tccccaggaa taagtttcca gaccggtaat
     1321 attgcttcca cgctgtgtcc attcactacc ggagtattca aagatccggg ccagtccggt
     1381 atagtcttcc ggaatactat agtaagggct gcccacagcc gcaacctggc cattggcatt
     1441 aagacttacg gcatagccgc aatattcacc ctcctcaagg ccggaaatat ctgaaccttt
     1501 ctgtgtccat tgactgaagc taataagtgg gaatgacagg atcagcatca aaaagatgtt
     1561 tcttcgcata gaacttgatg tttagttttt caagttccaa acatatctgc catgacttaa
     1621 tcatgcatca cgcctgcttg tgaaaggatc tatttcaccc ttacaagtga tggtatctca
     1681 tgtcatatga caattcaaag acaaaagtga tccgcaatat tcaggatgcc ggtttgcctg
     1741 acaatttgac agcttgcatc agcagggcta tttttttgta acgtgactcc ggcacctcaa
     1801 atatggcata catacggcgc agggatgacc ctatgccgtc tattgacaaa tgcacttctt
     1861 ctgagaggtc tttattggaa ataacaggat ttacaagcag tacattaagt actttccaat
     1921 cggtctcgtt cagtttacgc ttgagttggg cttcaatata ctgtctgttg aggaggaaag
     1981 catcactgga agttacagaa gctgtagcat tccacttcag ctgttctatt tctgccagta
     2041 acaaatctct ctgcctgcgg tgtttctggc ttcgaagacg aatatataca gatccggtaa
     2101 tgagcaataa tagtgtcacc agcaatatga aataaattcg tctttcttgt ttcaggcgaa
     2161 gtgtagcttg tgctttctca ttttccagct gtgttacaaa caatttttga tcaaattcgc
     2221 tctgtatggc ctgtctgata ataccgatct gatcttcagc aagttgaagg ctgtcactgt
     2281 attgctgata cctttccagc atgaaaaggg catctgccgg cctgcccaga gctttatagc
     2341 tctcatacag caaccttact gtgcttgctc ttaattccaa tctcggaata ccatcaattt
     2401 tccggtagat atcttcccct agagccattg cttcctgtgg attgctgcct gcgattagtg
     2461 cagcatacag caagctgcta ctgatgatac gttcttcgtt accaagagac tcactgatat
     2521 caaggcatag gctgatatag taaagtgcac tgtcctgttt gtttaggata ttataggtat
     2581 gtgacaattc atacatacaa tctgcactgg aaagcatatt atttttcctt tttaaaatag
     2641 gcaacgcttt gtagtaattt aataatgccg tttctgtatt tcccatagct aaataaacgg
     2701 cgcctgtatt caaccagatg tttccaacct cttcttcaag tcctatagag gcatacagat
     2761 ttcttgcctt ttccaggtat tcgagtgcca gtgggtacat ttgtacatcc tgatagatta
     2821 gtccgatgtt attaagcata tcagcacggg attcaggcag ggtatctagt tcgtttaaaa
     2881 taaaaaggct ctgcacatag atgtgaattg cttcctgata attgtccagt tgacggtaga
     2941 tgtttccaat gttagactgg atacgagcca ggccggctgc atctccaata gaagacaata
     3001 tactactagc ttctccaaga gcagtcagtg attggtctgc atcacttaga atataaaatg
     3061 caagtgcctt tttattaaga gcttcagcca tttctttggg attctgaagc tgtttggcca
     3121 gatcataatg ctttttcgtt actatgatac atgaatgtgg attactgaag atatatgcgt
     3181 tataataggt ggcaaatgct gtaaacctgg cagaatctga taatgtgata tcttcccaga
     3241 catgctgcaa ttgtaaacta tcagtttgag agatgcactg tgacccggta atgatcaatg
     3301 acttaagaaa tactgaaata gtaaatatca gcttccatct agccaatacc cggcttatat
     3361 tagaatttcg aggtgatctc atcgctattt gcagagggta aaattagtta gcttcccgca
     3421 ggatcttctc catcttcttc cccttggcca gttcgtccac cagcttgtcc atataccgga
     3481 tcttttgcat gagcgggtct tcaatatc
//
LOCUS       DAOMAX010000270         3504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5252_length_3504_cov_4.010438, whole genome shotgun sequence.
ACCESSION   DAOMAX010000270 DAOMAX010000000
VERSION     DAOMAX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5252_length_3504_cov_4.010438"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2241
                     /locus_tag="PK742_11380"
     CDS             <1..2241
                     /locus_tag="PK742_11380"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HQU77234.1"
                     /translation="QFKEFDPSSSLDFADILRPGNVDRLNEEMTVITSKSYKYNALQE
                     LPIEISLIASGRIRNTDMYYNGPFWVDIDVQDSTVLGDVIELRPIGDQKIRCAYEWKG
                     ETWSQELEYNKPYSTPAFSISVSRNGKGKVLAESYELIIRNKLTVAKAMEKRIRIDAE
                     NTYGGKIVIEYTDVNDQRAKDVVNTIADAIVTNGLERKSVSANSIISFIQSQIDSLEN
                     ELYIQENILKDFKKDAQLINPSIAEQNLMTKFNELDQNKLDVQLEERSLKWLLDYANN
                     NIEDLDALSGYFGNLKFNNFAPYFNSLSELEKQKANLMLSLPPTDPKVKLVTGQIDEV
                     KKNFKEALVNAQEQLEVRKNYLAEQERKYMGQFLQLPEKESEYMRLTRLSDIKEKYYL
                     LLKEKQAEYEITLAGLVSDYAVLDSATETVQVAPKVLQVWGIAIMAGLLLSFAFVYYQ
                     YLSNDTIFGVPDILRKTDISIAGVLPFHDDMQPGAPSVVVTNRATSIISESFRGLRTA
                     VLQHFSRLPATTEGRIIAVTSTVSGEGKTFVTANLSRSLALMGKRVIVVDYDLRKPKV
                     GKAFGEQAREGVASILNNNTDWHDKVIHNEQYQLDLLLCGPVPPNPAELLAGPGALAL
                     LEALKKEYDFVVVDNAPVGMVSDVIPLLSEVDINLYMIRAYFSRRRFIDNLDRLMEYH
                     GITNIQAVLNNIGEQQGYGEGAYSYGYGYGYGYGYGYGYGYGSDGYLEERKPKRGSIF
                     KRLFRK"
     gene            complement(2287..>3504)
                     /locus_tag="PK742_11385"
     CDS             complement(2287..>3504)
                     /locus_tag="PK742_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU77235.1"
                     /translation="LRDTGNTVLVVEHDKEMMIQSDYIIDLGPGAGIHGGRVVASGDP
                     VSFMKQHSQTAEYLNHRKVIEIPEKRRDGNGKQVLLTGARGHNLQKVDLHLPLGKLIC
                     VTGVSGSGKSSLINGTLYPLMMQHYYHSNRKPLPFNSILGLEHLDKVIEIDQAPIGRT
                     PRSNPVTYINVFTEIRQLFTLVPEAKIRGYKPGRFSFNVKGGRCEHCEGGGMRVIEMN
                     FLPDVEVECEVCRGKRYNRETLEIYYKGKNIADVLDMTVEQAVAFFEHIPKIYRKMKT
                     LEEVGLGYIRLGQSSTTLSGGEAQRVKLAEELSRKDTGNTFYILDEPTTGLHFEDIRV
                     LMQVINSLVDKGNTVLIIEHNLDVIKMADHIIDLGPEGGSGGGRIIATGTPEQIASHQ
                     HSHTGHYLADELK"
BASE COUNT          947 a          790 c          836 g          931 t
ORIGIN      
        1 cagttcaaag aatttgatcc cagcagtagt cttgattttg cagacatact tcgcccaggt
       61 aatgtggacc ggctcaatga agaaatgacc gtcattactt ccaaatcata caagtataat
      121 gcacttcagg agcttccgat agagatatca ctgatcgctt caggacggat tcgtaataca
      181 gatatgtact acaacgggcc gttctgggtg gatatagatg tacaggacag tacggtgttg
      241 ggagatgtca ttgaacttcg tcctattggc gatcagaaga tccgttgtgc ttatgaatgg
      301 aaaggagaaa catggtcgca ggaactggag tataacaagc cgtatagcac cccggcattt
      361 agtatatcag tatccagaaa tgggaaagga aaagtactgg cggagtctta cgaactgatc
      421 atccgaaata aactgactgt agccaaggca atggaaaaaa ggatccgtat cgatgccgag
      481 aatacatacg gaggtaagat cgtcatagaa tatactgatg tcaatgacca gcgggccaag
      541 gatgtggtaa acaccattgc agatgctatt gtaactaatg gcctggaaag aaaatcagtt
      601 agtgccaact ccattatcag ctttattcag tcacagatcg attcattgga gaatgagttg
      661 tacatccagg agaacatcct caaggatttt aagaaagatg cacaactgat caacccttct
      721 attgctgagc agaacctgat gaccaagttc aatgaactcg atcagaataa actggatgtg
      781 cagctggaag aaagaagcct gaagtggtta ctggattatg ccaacaacaa tattgaagat
      841 ctggatgcct tatcaggtta ttttggaaac cttaagttca acaatttcgc accttacttt
      901 aattctcttt ctgagctcga gaaacagaaa gcaaatctga tgctgagcct gccgcctaca
      961 gatcccaagg tcaaactggt aaccgggcag atagatgagg tgaagaagaa tttcaaagaa
     1021 gcactggtca atgcacagga acaactggag gtcagaaaga actatctggc agaacaggag
     1081 cgcaaataca tgggtcagtt cctccagtta ccggagaaag aaagcgagta catgaggctt
     1141 acccgattaa gcgatatcaa agagaaatat tacctgttgc tgaaggaaaa acaggctgag
     1201 tatgagatca cgctggcagg tctggtgtct gactatgcag tgctggatag tgcaacagaa
     1261 acagtgcagg tagcacctaa ggtactgcag gtatggggta ttgctatcat ggccggcctt
     1321 ctgctttctt tcgccttcgt gtactatcag tatctaagta atgataccat tttcggtgtg
     1381 cctgatattc ttagaaaaac tgatatatct attgcaggag tactgccgtt ccatgacgat
     1441 atgcaacccg gggcgccttc ggtagtggta accaaccgtg ccacctcgat catttctgaa
     1501 tctttcagag ggttgcgaac tgccgtgcta caacatttct cacgacttcc tgcaaccacc
     1561 gagggtagga tcattgctgt aacttccacc gttagtggag agggtaaaac atttgttacg
     1621 gccaacctga gccggtcgct ggcattgatg ggaaaacggg tcatagtggt tgactatgac
     1681 ctcagaaaac cgaaagtggg aaaagctttc ggtgaacagg ctcgtgaagg ggtagctagt
     1741 attctgaata ataatacgga ctggcatgac aaggtcattc acaatgaaca atatcagtta
     1801 gacctcctgc tttgcgggcc ggtacctccc aacccggctg aactgctggc tggtcccggg
     1861 gcactggcgc tgttggaagc attgaagaaa gagtatgact ttgttgtggt agataatgca
     1921 cctgtgggga tggtgtctga tgtcattccg ctgttgtctg aagtggatat caacttatat
     1981 atgatcagag cctatttctc caggagaaga tttattgaca atctggacag gctcatggag
     2041 tatcatggta tcaccaatat tcaggcggta cttaataata tcggcgaaca gcaggggtac
     2101 ggagaaggcg cctacagcta tggctacggc tacggttatg gctatggata cggttatggc
     2161 tacggctatg gttctgatgg ctatctggaa gagcgtaaac ctaaaagagg aagtatcttt
     2221 aaaagactgt tccgtaagtg aacctgctgc gtataaactt ttgaggcgac caactggtcg
     2281 cctttttcat ttcagttcat cggccaggta gtgacccgta tgactgtgct ggtgcgaagc
     2341 gatctgctca ggtgtgcctg tagcaatgat gcgtcctccg ccactgccac cctccggacc
     2401 cagatcaatg atatgatcag ccattttgat cacatcgagg ttgtgttcaa tgatgagtac
     2461 tgtatttcct ttatcaacca ggctgttgat gacctgcata agcaccctga tatcttcaaa
     2521 atgcagtccg gtggtaggct cgtccagtat ataaaaggtg tttccggtat ctttccggct
     2581 cagctcttcg gccagcttga cccgttgtgc ttcaccacct gataaagtgg tgctggactg
     2641 ccccagtctg atataaccca ggcccacttc ctccagcgtt ttcatcttgc ggtagatctt
     2701 cggaatgtgt tcgaaaaatg ctaccgcctg ctctacggtc atatcaagca cgtcagcaat
     2761 gttcttgcct ttatagtaga tctccagggt ctctctgttg tatcgttttc cgcgacagac
     2821 ctcacattcc acttccacat caggcagaaa attcatttct atcacccgca tacctccacc
     2881 ttcgcagtgt tcgcaacggc ctccttttac attgaaagaa aaacgacctg gcttatatcc
     2941 acggatctta gcttcaggta ccaaagtaaa tagctgcctg atctctgtaa aaacattgat
     3001 ataagtgacc ggattgcttc tcggtgttct gccaataggc gcctgatcga tctcgatcac
     3061 tttatccaga tgttccaaac ccagtatgga gttgaaaggc agcggtttcc tgttggaatg
     3121 gtaataatgc tgcatcatca acgggtacaa agtgccgttg atcagacttg attttccgct
     3181 acctgataca ccagtcacgc agatcagttt acccagtgga aggtgcagat ctaccttctg
     3241 cagattgtgc cccctggctc cggtcagcag tacctgcttg ccgtttccat cccgcctttt
     3301 ttcagggatc tctatgacct ttctgtgatt taggtattcg gctgtttgag aatgctgttt
     3361 cataaacgaa accggatcgc cgcttgccac aacccgtcct ccatgaatac ctgcacctgg
     3421 cccaaggtct atgatgtagt cagattgtat catcatttcc ttgtcgtgtt ccaccacaag
     3481 aactgtattt ccggtgtctc tgag
//
LOCUS       DAOMAX010000271         3498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5267_length_3498_cov_4.414174, whole genome shotgun sequence.
ACCESSION   DAOMAX010000271 DAOMAX010000000
VERSION     DAOMAX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5267_length_3498_cov_4.414174"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..561)
                     /locus_tag="PK742_11390"
     CDS             complement(145..561)
                     /locus_tag="PK742_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQU77236.1"
                     /translation="MDKSGFQEQLKEKGLKVTPQRMIIYEAVVTMKNHPTADNIISYV
                     KQHHPGISVGTVYKVLDSFVENHLLRKVKTEKDVMRYDPILHQHHHLYCYDTDRIEDY
                     EDPELDQLIAQYFEKKKIKGFDLTNITLQITGKFKK"
     gene            722..1111
                     /locus_tag="PK742_11395"
     CDS             722..1111
                     /locus_tag="PK742_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQU77237.1"
                     /translation="MHPDILGYHQQLAATDQAICEALHKAICRALPDAENKIWHAHPV
                     WFLDGNPITGYSKLKDSVRLLFWSGQSFDEPGLQPEGKFKAAEKRYTEADQIDTEELI
                     RWLEKSRAIQWDYKNIVKRKGRLERLV"
     gene            1134..1481
                     /locus_tag="PK742_11400"
     CDS             1134..1481
                     /locus_tag="PK742_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQU77238.1"
                     /translation="MLTAIHPKLPMRNLSATRNYYVQQLGFEEVADYGNYFIVRKDDI
                     EIHFFLHEHLDTKTNDGQVYIRTDAIDALYADMRSKTTIHPNGALQTHSWGMREFSLL
                     DPDHNLLTFGQPV"
     gene            1496..1939
                     /locus_tag="PK742_11405"
     CDS             1496..1939
                     /locus_tag="PK742_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU77239.1"
                     /translation="MYIREATLSDISAMQIVRRAVRENALSDPTRISDEDYVDFLTSR
                     GKGWVAIENDEVNGFAIADLQEHNIWALFVHPDHEAKGIGLALHDQMLNWYFSQTNTT
                     VWLSTAPGTRAERFYHKAGWTLTGYYGQGELRFEISSKVWKTLHQ"
     gene            1981..2610
                     /locus_tag="PK742_11410"
     CDS             1981..2610
                     /locus_tag="PK742_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77240.1"
                     /translation="MQRIVLFACLSLWLSSVTMAQRFEGSVTYEINYIQVPEDMAAYK
                     SMLPNSMTMYFLGEQARMEQSVMGSLQVVVSNVKDNSGFVLTDFMGMKIAMPIEPTLN
                     NTEDDVQYEYTDERKEIAGYACKKAIVTMEGMEPSVIYYTKEISGYHQDYTGLDGYPL
                     QYEISQDGMVFTVTANNVSKEKPDAQLFIIPDDYSVMTQEELMRSFGGE"
     gene            2678..3235
                     /locus_tag="PK742_11415"
     CDS             2678..3235
                     /locus_tag="PK742_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77241.1"
                     /translation="MLSLFLIFHSGGQLEAQSFTDELTTESTRQYPAGGSDTVILEQF
                     DKHFLESDQAYIGLEPDSRWQIMFTYSYEWEEIVGRPADISYDPDHNERSGLTTKRYY
                     YQAAVFDSDELTDGLNIPLDLQDSGKKPYRDMPLFYDRGAPRNGVWIPIRSGDYLMMR
                     LAHNYPNGNMTSFYREYRFYFKKLK"
     gene            3239..>3498
                     /locus_tag="PK742_11420"
     CDS             3239..>3498
                     /locus_tag="PK742_11420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HQU77242.1"
                     /translation="MYLLHGKLTAKKGQEEALAKILLQASELVAGAAGCRLYVISNDE
                     QDPQQVYVTEIWDDKTAHDASLQMPEVRQLIQQAMPLLDGMPT"
BASE COUNT          958 a          808 c          827 g          905 t
ORIGIN      
        1 taaggatcgg tcttggtgtc gtgctggtgc aggatgtcca ggttcagcgt cttcggccac
       61 cagtctgtca ccgcatgctg ggtctccgtg ttcgccccgt gatggaaggg gcattttccg
      121 ttctttgttt ccattttata tagtttattt tttaaacttt ccggttatct gtaaagtaat
      181 attggtcagg tcgaaacctt tgatcttctt cttctcaaaa tactgagcaa tgagctggtc
      241 gagttccgga tcttcatagt cttctatccg gtctgtgtcg tagcaataga ggtggtgatg
      301 ctggtggagg atgggatcat aacgcatcac atccttctcg gtcttgacct tgcgcagcag
      361 atggttctct acaaaagagt ccagcacttt atagaccgtg ccgacagaga tgccgggatg
      421 atgttgtttg acgtagctga tgatgttatc ggctgtggga tggttcttca tcgtgaccac
      481 cgcttcatag atgatcatgc gctggggcgt gaccttcagt cccttctcct tcaactgctc
      541 ctgaaacccg gacttatcca tcagattatt agcgagaatt attcgccaaa gataatgatt
      601 cttatttaga gtttctatca gatcttattc ccagcccgaa tttctgctct tgatgagttg
      661 cctgtaaagg ccgaaaattt tgccattctt accttacttt tactcacttc aatctcaacc
      721 aatgcatccg gacatccttg gctatcacca gcaattagct gccacagacc aggccatttg
      781 tgaggccttg cacaaggcca tatgcagagc ccttccggat gccgagaaca agatctggca
      841 tgcacacccc gtatggttcc tggacggtaa cccgataacc ggctacagca aactaaagga
      901 ctctgtcagg ttgcttttct ggagcgggca atcgttcgat gaacccggct tgcaaccgga
      961 aggaaaattc aaggcggcag aaaaaagata tacagaagca gatcagatag atacagaaga
     1021 gctgatcaga tggctggaaa agtccagggc cattcagtgg gactacaaga atattgtgaa
     1081 acgaaaaggc aggctggaac gtcttgtctg aaagatcttt aaacgaacag tacatgctga
     1141 ctgccatcca tccgaaactt cctatgcgca acctcagtgc gacccggaat tattatgtac
     1201 aacaattggg cttcgaagaa gtagctgact acggcaacta ttttatagtt agaaaagacg
     1261 atatcgagat ccattttttc ctgcatgaac acctcgatac gaaaacgaat gatggccagg
     1321 tctatattcg cactgatgcg atcgatgcct tgtatgcaga catgcgttca aagaccacga
     1381 tccatccgaa cggagctttg cagacacact cctggggcat gcgggagttt tccctcctcg
     1441 atcctgatca caacctgctt acattcggac agccggtctg agcttacctg ttggcatgta
     1501 tattcgtgaa gcaactttat ctgatatctc cgcaatgcag atcgttcgaa gagcagtccg
     1561 tgaaaatgcc ctgtctgatc ccacacgcat cagcgatgaa gattatgtgg actttctgac
     1621 cagcagagga aaaggctggg tggcgataga aaatgatgag gtaaacggat tcgctattgc
     1681 agatctgcag gaacacaata tctgggcttt gttcgtacac cctgaccatg aagcaaaagg
     1741 aatcggcctt gctttacatg atcagatgct caactggtat ttctctcaga ccaatactac
     1801 tgtatggctc agcactgcac caggcacaag ggcagagcgc ttttaccaca aagccgggtg
     1861 gacattgaca ggctattatg gtcaggggga gctaagattt gaaatctctt caaaggtctg
     1921 gaaaaccttg catcagtagc ttccgaatat ggattgcgta tctttagaaa aaattagatc
     1981 atgcaaagaa tagttctgtt tgcctgtctg tcactgtggc tgtcttccgt gaccatggca
     2041 cagcgttttg aaggttctgt tacctatgag atcaactata tccaggttcc tgaagacatg
     2101 gcggcctaca agagcatgct tcccaacagc atgaccatgt attttctggg cgaacaagcc
     2161 aggatggaac aatctgttat gggttcactg caggtagttg taagcaatgt caaagacaac
     2221 agcggatttg tgctgacaga tttcatgggg atgaagatcg ctatgcccat tgagccgacc
     2281 cttaacaata ctgaagatga tgtgcagtat gaatatacag atgagcgcaa ggaaattgca
     2341 ggatatgcct gtaaaaaagc catcgtaact atggaaggaa tggaaccatc tgttatttat
     2401 tataccaaag agatatcagg ttaccatcag gactacaccg gactggatgg atacccgtta
     2461 caatatgaga tcagtcagga cgggatggta tttacagtaa ctgcaaataa tgtaagcaaa
     2521 gaaaaacctg atgcacagct gttcattatt cccgatgatt attccgtcat gacccaggaa
     2581 gaactgatga ggtcttttgg tggcgaatga cccgaggtct ccatctgaaa gtccgggtaa
     2641 tttattgaat ttgttgatgt gcagaatcat tcaactgatg ctttcattat ttctgatctt
     2701 ccactcagga ggtcaactgg aagcgcagtc cttcactgat gaactgacca ccgaatccac
     2761 cagacaatat ccggcaggcg gttctgatac cgtcatcctg gaacagtttg acaaacactt
     2821 tctggaatca gatcaggcat acatcggtct cgaacccgac agtcgctggc agatcatgtt
     2881 cacctattcc tacgagtggg aggagatagt gggtcgtcct gctgatatca gctacgaccc
     2941 cgaccacaac gaaaggagcg gactcaccac caaacgctac tactaccagg cggccgtatt
     3001 cgattctgat gaactgaccg atggcctgaa tattccgctg gatctgcagg acagcggcaa
     3061 aaaaccttac agagatatgc ctctgttcta tgaccggggt gcaccacgaa atggcgtttg
     3121 gatacccatt cgttccggcg actatctcat gatgcgactg gcacataact atccgaacgg
     3181 caacatgacc agtttttaca gagagtatcg gttttacttc aaaaaactaa aatgacctat
     3241 gtacctcttg cacggaaaac taacggctaa aaaaggacag gaagaggccc ttgcaaaaat
     3301 tctactccag gcatcagaat tggtggcagg tgctgccgga tgcaggcttt atgtgatcag
     3361 caatgatgaa caggacccgc agcaggtgta tgtcactgaa atatgggatg ataagaccgc
     3421 ccatgatgcc tcattacaaa tgcctgaagt acggcagctc attcaacagg ctatgccact
     3481 attggatggt atgccaac
//
LOCUS       DAOMAX010000272         3498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5269_length_3498_cov_3.540517, whole genome shotgun sequence.
ACCESSION   DAOMAX010000272 DAOMAX010000000
VERSION     DAOMAX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5269_length_3498_cov_3.540517"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PK742_11425"
     CDS             <1..728
                     /locus_tag="PK742_11425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQU77243.1"
                     /translation="MQHDEPVPVTLHVEAGTMVSFPTADGKDARAYVVRPDQAGSNFL
                     IVFHEWWGLNDHIIQMADQLAADLKDVTVIAVDLYDGQVAATREDAASAMQSMSTERG
                     IHIIRGVQQLVQNQYTDEQPTFATIGWCMGGGWSLQAALEYRELSSACVIYYGMPESD
                     ADRLSQLECPVLFVFAKQDKWISYDVVSGFKKNMLHAGKKLRILEYDADHAFANPSNP
                     GYNNEAALEAYAESLSFLYTALH"
     gene            complement(725..1027)
                     /gene="trxA"
                     /locus_tag="PK742_11430"
     CDS             complement(725..1027)
                     /gene="trxA"
                     /locus_tag="PK742_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQU77244.1"
                     /translation="MKASFSELISGEQPVLVDFTAAWCGPCKMLAPILEEVAGKLGND
                     VRIIKIDVDQNPAVAGRYQIRGVPTLILFRRGEILWRQSGVQSAHALEQAIRGVLV"
     gene            complement(1078..2019)
                     /locus_tag="PK742_11435"
     CDS             complement(1078..2019)
                     /locus_tag="PK742_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQU77245.1"
                     /translation="MEKKLIYGCMGLGGSWSHDPLTADDQQHADVALQAAMECGISHF
                     DHADIYTLGKAEAAFGNWYRKAGADRSSVIIQSKAGIRIGVGRNGSNVYDLSRNYLLE
                     SVQRSIERLQCEYLDIFLLHRPDPLMDAGEVADTFAEMQERGFVKTFGTSNFPFSVVR
                     SIQHYWKDPMRTHQLQGSLMHDHLIAHLVDAHTRAYQHADGSEGWWGAFLKDTYDWQV
                     WGATDRGRFTGGYRDGMDQQDEAIVVKVRELAASYETSTYAIVLAWLMKLPVSIQPLI
                     GSTDPERIRMSAQSLSVQLSKEHWYDLWLTARNRPLP"
     gene            complement(2004..3011)
                     /locus_tag="PK742_11440"
     CDS             complement(2004..3011)
                     /locus_tag="PK742_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQU77246.1"
                     /translation="MYRSFWYLPLILILFFGATSSGFEPEVPEGFPPVVYPEDNTFTA
                     DRYELGRRLFYDPILSADSSISCASCHLPELAFSDQVALSPGIYGRPGVRNAPSLANV
                     AYHPYFLREGSVPTLEQQVLVPIQEENEMAHNIVAIASAMERDSSYVAMSISAYNRPP
                     DAFVITHALATFERTLLSGNSRYDQLMRQEEGAAFGQLERQGMELFFSDRTNCSVCHG
                     GFNFTDYRFANNGLDTSYADIGRMRFTGKQEDLSLFKVPSLRNIALTAPYMHDGSIAT
                     LEEVVHHYNTGGKAHPHRDPLIRPLHLSSEEEQAIVAFLKTLSDPDFVSSAKATVWRK
                     N"
     gene            complement(3014..>3498)
                     /locus_tag="PK742_11445"
     CDS             complement(3014..>3498)
                     /locus_tag="PK742_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQU77247.1"
                     /translation="RGELELTESENRGRELFFAEYNPYFPDLSGADCAHCHSGSNFEN
                     DLYMNNGLDSDADLLDAGRELVTGDPADKGRFKVPTLRNIEVTFPYMHDGRFSTLEEV
                     LDHYNDGLQLSASLEPQLAYTMETGLMLSEEDKADLIAFLKTLTDQSLLNDPKYASPF
                     "
BASE COUNT          891 a          875 c          820 g          912 t
ORIGIN      
        1 tgatgcaaca tgatgagcct gttcctgtaa cgctacatgt cgaagcagga actatggtat
       61 cttttccaac cgccgatggg aaagatgccc gtgcctatgt ggtaagacca gatcaggctg
      121 gcagtaattt tctgatcgta ttccatgaat ggtggggtct taatgaccat atcatacaga
      181 tggcagatca gctggctgca gatctgaagg atgtgaccgt cattgctgta gatctgtatg
      241 atggtcaggt ggccgctacc cgcgaagatg ctgccagtgc catgcaatca atgagcacag
      301 aaagaggtat tcatatcatc cggggagttc agcagttggt tcagaaccag tacacagatg
      361 aacagcccac ttttgctacc ataggatggt gcatgggtgg gggatggagt ttgcaggctg
      421 cccttgagta ccgagaatta tcttcagcct gtgtcattta ctatggtatg cctgaatcag
      481 atgccgacag actatcacaa ctggaatgtc cggtattgtt cgtttttgcc aaacaggaca
      541 agtggatcag ttatgacgtg gtttccggat tcaaaaagaa tatgcttcat gctggaaaaa
      601 aactcaggat actggaatat gatgcagatc atgcgtttgc caacccaagc aaccctggtt
      661 ataacaatga agcggcactg gaagcttatg cagaaagcct gtcttttctc tataccgcac
      721 tgcactagac cagcacacct ctgatggcct gttcaagtgc atgtgcagat tgcacacctg
      781 actgacgcca cagaatttct ccacgtctga agaggatcag ggtaggcact ccgcggatct
      841 ggtaacgacc tgctacagca gggttctgat caacatcgat cttgatgatg cgcacatcat
      901 tgccaagttt acctgctacc tcctccagta taggtgcaag cattttgcag gggccgcacc
      961 aggcggctgt aaaatcaacc agaaccggct gttcgccgga gatgagttca gaaaaagatg
     1021 ccttcatgca aacaaaaata aatggaagtg cctgtctgag aagtgatgga agtcacttca
     1081 tggtaaagga cggtttcggg ctgtaagcca gaggtcatac cagtgttcct tactcaactg
     1141 caccgacaaa ctttgtgctg acattctaat tcgttccgga tctgttgaac ctatcaatgg
     1201 ttgaatactt accggaagtt tcattagcca tgccagcacg atcgcatagg tacttgtttc
     1261 ataggaggcg gccaattctc ttactttcac aacaattgct tcatcctgct gatccatacc
     1321 atcccggtat cctccggtaa accttccccg gtctgttgca ccccacacct gccagtcata
     1381 tgtgtctttc agaaaagcac cccaccatcc ctcactacca tcggcatgct gataagccct
     1441 tgtatgagcg tccacaaggt gagcgatcag atggtcatgc atcagactgc cctgtagctg
     1501 gtgtgttcgc atagggtcct tccagtaatg ctgtatggat ctgaccactg aaaacggaaa
     1561 gttgctggta ccaaaagtct ttacaaatcc acgttcctgc atttctgcaa aggtgtctgc
     1621 tacttctcct gcatccataa gcggatctgg tctatgcaac agaaaaatat ccaggtattc
     1681 gcattgcagt ctttctatac ttcgctgtac tgactccagc agatagttcc tgctcaggtc
     1741 gtacacattg gagccgtttc ttccgacacc gatccggatt cccgctttgc tctgtatgat
     1801 caccgaagac cggtcagcac ccgcttttcg ataccagttt ccgaatgcag cttcagcttt
     1861 tcccagtgtg taaatatctg cgtggtcaaa gtggctgata ccacattcca tggccgcctg
     1921 aagagccaca tctgcatgtt gctgatcatc ggctgtaagt ggatcatgac tccagcttcc
     1981 ccccaacccc atgcaaccat atatcagttt tttctccata cggtagcttt tgctgaagac
     2041 acaaaatcag ggtcagacag tgtttttaaa aaggctacaa tagcctgttc ttcttctgat
     2101 gaaagatgca gcggccgtat aagcgggtcg cgatgggggt gggctttacc gccggtatta
     2161 taatggtgca ccacttcttc cagtgttgct atactaccat catgcatata aggcgctgtc
     2221 agggcaatat tgcggagaga cggcaccttg aacaaggaaa gatcttcctg tttaccggtg
     2281 aagcgcatcc ttcctatatc agcatagctt gtatccagtc cgttgttggc aaagcggtaa
     2341 tcggtgaagt tgaatccacc atggcataca gagcaattgg tcctgtcact gaagaacagt
     2401 tccatccctt gtctctccaa ttgaccgaac gccgctccct cctcctgcct catcagctga
     2461 tcatatctgc tgtttccact cagcagtgtg cgttcaaaag tggccagggc atgggtgatc
     2521 acaaatgcat ccggcggacg attataggcg ctgatactca tggccacata gctgctgtcg
     2581 cgttccattg cggatgcaat agccacgata ttgtgtgcca tttcattctc ctcctgaatg
     2641 ggaaccagca cttgttgttc cagagtgggt acactgcctt cccgaagaaa atacgggtga
     2701 taggccacat tagctaaaga cggtgcattc cgcacacccg ggcgaccgta aattcccgga
     2761 ctcaatgcca cctgatcact gaaagccagt tccggcagat gacaggaagc acaggatatg
     2821 ctgctgtctg ccgagaggat cggatcatag aagagtcttc ttcccaattc ataacggtct
     2881 gctgtgaagg tattgtcttc aggatagacc actggtggaa aaccctccgg cacctcgggc
     2941 tcaaatcctg aagatgttgc cccgaagaac agtatcaaaa taagcggaag gtaccagaat
     3001 gacctgtaca tgttcagaaa ggtgaggcgt acttggggtc gttgagaagg ctttgatctg
     3061 ttaatgtttt cagaaaagct atcagatcag ctttgtcttc ctccgacagc atcagacccg
     3121 tttccattgt ataggcaagt tgtggctcca atgatgcaga caattgcagg ccgtcgttgt
     3181 aatggtctaa tacttcttct aaggtagaga acctgccatc gtgcatataa ggaaaggtaa
     3241 cctctatatt gcgcagagta ggcactttga accggccttt gtcagcagga tcgccggtaa
     3301 ccagctctct tccagcatcc agcaaatcag catcagaatc caacccgtta ttcatataca
     3361 ggtcgttttc aaaattagaa ccgctgtggc agtgtgcaca gtcagcaccg ctcaggtcag
     3421 gaaagtatgg attgtattct gcaaaaaata attcacgacc tctgttttcg ctttcggtca
     3481 attccagttc acctctta
//
LOCUS       DAOMAX010000273         3466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5355_length_3466_cov_4.418939, whole genome shotgun sequence.
ACCESSION   DAOMAX010000273 DAOMAX010000000
VERSION     DAOMAX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5355_length_3466_cov_4.418939"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="PK742_11450"
     CDS             complement(<1..1829)
                     /locus_tag="PK742_11450"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQU77248.1"
                     /translation="MYLNCHTYFSLRYGTLSPDKLLQEAVAHEVSRFVLADINNTSGI
                     FETVRKARQLGITVIPGIEFRNGARHLFTGIARSEAGFTALNRFLSGHLNSGEPLPEL
                     APALPDTLIIYPFEPGRTFRLRAHEYIGVRPGQLHQLTRYAGRYQQEKLVALHPVTFH
                     NRKDFHAHRILRAVDNNTLLSKLDPAALARSDEQFLLPDEMRSQYAQHPYLLHHANAL
                     LDQCDFSYVFGQNRNKRFFSGSETEDADILLQQTFEGVRRRYGSVNKTIHERINKELD
                     IIVKKGFVSYFLINWDIVEYARRQGYFTIGRGSGANSLIAYCLGITDVDPIDLDLYFE
                     RFINLFRETPPDFDLDFSWTDRDDVTRYIFDRYGHEHTALLGTYSEFKEKAFIREVAK
                     TFGLPKQEIDDLIKAYGEDPEKVKRNIPDKLAKSIYNYGEYIYRFPNHLGIHASGILI
                     SDSPIYQYSATYLPPKGFPTTQFDMYVAEDIGLYKFDILSQRGLGHIKDSIRLIRQNK
                     QAEVDIRQVRKLKEDPKLNERLASGNTIGCFYIESPAMRMLLRKLQVSDYLTLVAASS
                     IIRPGVARSGMMREYIMRHRFPEERKRMHPVLGDIMPDTYGVMV"
     gene            complement(2064..3251)
                     /gene="dinB"
                     /locus_tag="PK742_11455"
     CDS             complement(2064..3251)
                     /gene="dinB"
                     /locus_tag="PK742_11455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQU77249.1"
                     /translation="MRSIVHMDLDTFFVSVERLKDSRLLGKPVLVGGSSDRGVVAACS
                     YEARAFGIHSAMPMRTARALCPEAILVRGDHEHYSYYSNMVTDIIEEAVPSYEKSSID
                     EFYIDMSGMDRFFGTWQYVQELREKIIRETGLPISMGLSENKTVSKVATGEAKPNNKI
                     KVDHGTEKPFLAPLAVSKIPMVGERTSETLRRMGIVRISTVQDMPMRVMENILGENGR
                     SIWKKANGMDSSPVEPYTERKSISTEETFDSDTTDVKQLRNILIAMTEKLAYQLRKEN
                     KLASVVTVKLRYSDFETHTRQVRIPYTSTDHALIRVVKELFDKLYERRVLIRLVGVRL
                     SGLVHGGYQINLLEDSEALIHLYQAMDRMRRKYGKTSIQRAVAMGQRFHEFNPFNGRR
                     AEG"
BASE COUNT          693 a          873 c          957 g          943 t
ORIGIN      
        1 accatcacac cataggtgtc tggcatgata tcgcccaaca cagggtgcat acgtttgcgt
       61 tcttccggaa aacgatgacg catgatatac tcccgcatca taccggaacg ggctacgccg
      121 gggcggatga tggaactggc cgctaccagg gtcagataat cgctcacctg cagtttgcgc
      181 aacaacatgc gcatggccgg cgactcaatg taaaaacagc cgatggtatt tccgctggca
      241 aggcgttcat tgagcttcgg atcttccttc agtttgcgca cctgacggat gtccacttcg
      301 gcttgtttgt tctggcggat cagccggatg ctgtctttga tatgccccaa cccccgctga
      361 ctcagaatat cgaatttgta caacccgatg tcttcggcca catacatatc gaactgggtg
      421 gtggggaagc ctttgggcgg caggtaggtg gcgctgtact ggtagatggg gctatctgat
      481 atcaggatgc cgctggcatg aatgcccagg tgattgggga agcggtagat gtattcgcca
      541 tagttgtata tactttttgc aagcttgtca ggaatgttgc gcttcacttt ttcagggtcc
      601 tctccgtagg ctttgatgag gtcatcgatc tcttgtttgg gaaggccgaa ggtcttggct
      661 acttcgcgga taaatgcctt ctccttgaac tctgaataag tacccagcag ggcggtgtgt
      721 tcatgcccgt aacggtcgaa gatgtatcgg gtcacatcgt cgcggtcggt ccaggaaaag
      781 tccagatcga agtcgggcgg tgtttcgcgg aagaggttga tgaagcgttc gaagtagagg
      841 tccaggtcta tgggatctac atcggtgatg cccaggcagt aggcgatgag actgttggca
      901 ccactgccac ggccgatggt gaagtaaccc tgccgacggg catactccac gatgtcccag
      961 ttgatgagaa agtaagacac gaaccctttt ttgacgatga tgtcgagctc cttgttgatg
     1021 cgctcgtgga tggtcttgtt cacacttccg taccgacggc gcacaccttc gaaggtctgt
     1081 tggagcagga tgtctgcatc ttcagtttca gaccccgaga agaatcgctt gttgcggttc
     1141 tgcccgaaca cataagaaaa atcacactga tccagcaggg cattggcatg gtgcagcagg
     1201 taaggatgct gcgcatactg cgaacgcatc tcatccggta gcaggaactg ctcatcgctg
     1261 cgggccagtg ctgccggatc cagtttactc agcagggtgt tgttatctac agcccggaga
     1321 atgcggtggg catgaaagtc tttccggttg tggaaggtca ctggatgcag ggccacgagt
     1381 ttttcctgct gataccttcc tgcatagcgg gtcagctgat gcagttgtcc gggtctgaca
     1441 ccgatgtatt catgtgcccg caggcggaag gtgcgtccgg gttcgaacgg atagatgatg
     1501 agggtgtccg gcagggcagg agcgagctcc ggaagtggtt ctccgctgtt gaggtgtccc
     1561 gacagaaaac ggttcagggc ggtaaagcct gcttcactgc gggcaatgcc ggtgaacaga
     1621 tgccgggctc cgttgcggaa ttcaatgccg ggtatcacag tgatgcccag ttgccgggct
     1681 ttacgcaccg tttcaaaaat gccggaggtg ttgttgatgt cggccagcac gaatcgcgat
     1741 acttcatgtg ccactgcttc ctgcaacagc ttgtcgggcg acaaggtccc gtagcgcagg
     1801 ctgaagtatg tgtggcagtt gaggtacatg gacgtggtgc gttattcgtt gttcgttttg
     1861 cgttattcgt ttttggttat tagttattgg tttggctgcc tttacctgat accagctact
     1921 tgatacccga tacttttttt taatgagcga ctcaccattc accgctcaca tttcgttttt
     1981 agtccttggt tgttagttat tggtttagtt gcccttaccc gatacttgtt tatgccaact
     2041 gccaactgcc actgcccatt ctttcacccc tccgccctcc ttccgttgaa cggattgaac
     2101 tcatgaaacc gctgtcccat ggccacggct ctctgtatgc ttgtcttgcc gtacttgcgg
     2161 cgcatgcgat ccatggcctg atagaggtgg atgagggctt cagaatcttc cagcaggttg
     2221 atctggtatc cgccgtgcac cagaccactc agccgtactc ccaccagtct gatcagcacc
     2281 cgtcgttcat acagtttgtc gaacagttcc ttcaccacgc ggatgagggc gtgatccgta
     2341 gaagtatagg gaatgcgcac ctgccgggta tgggtctcga aatcggagta gcgcagctta
     2401 acggtcacca cagaagcgag cttgttctcc ttgcgcaact ggtaagccag cttctctgtc
     2461 atggcgatga gaatgttgcg caactgcttt acatcggtag tatcgctgtc aaaggtctct
     2521 tcggtcgata tggatttgcg ttcggtatag ggttctaccg gcgagctgtc catgccgttg
     2581 gccttcttcc agatgctgcg tccgttctct cccagtatat tctccatgac ccgcatgggc
     2641 atgtcctgca cggtagagat gcgcacgatg cccatacgcc ggagggtctc ggaggtgcgc
     2701 tcgcccacca tcggtatctt gcttactgcc agcggtgcga gaaagggttt ctccgtgccg
     2761 tggtccacct tgatcttgtt attgggcttg gcctcacccg tagccacttt ggatacggtc
     2821 ttgttctccg ataatcccat ggagatgggt agtcccgtct cccggatgat cttctcccgc
     2881 agctcctgta catactgcca tgtgccgaag aagcggtcca taccactcat atcaatataa
     2941 aattcatcta tagatgattt ttcataggat ggaacagcct cttcaatgat gtcggtcacc
     3001 atgttggagt aataggagta gtgttcgtga tcaccccgca ccagtatggc ttccggacat
     3061 agtgcacggg cagtacgcat gggcatggca gaatggatac caaaggcacg ggcctcataa
     3121 ctgcaggcgg ccactacgcc acggtcgctg ctgccaccca ccagcacggg cttgcccagc
     3181 aggcgactgt ctttgagccg ttctactgac acgaagaatg tgtcgaggtc catatgtacg
     3241 atagagcgca tggaggagat tcgttgttcg ttgttcgttt tgcgttattg gttattcgtt
     3301 attcgttttt ggttattggt ttggctgcct ttacccgata ctatttcatt actgctgtcg
     3361 cagccaactg cttactgctt tactcgttgt tcgttgttcg ttattcgtta ttcgttattc
     3421 gttattcgtt tttggttatt agttattggt cattggtcat tggttg
//
LOCUS       DAOMAX010000274         3436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5456_length_3436_cov_5.009760, whole genome shotgun sequence.
ACCESSION   DAOMAX010000274 DAOMAX010000000
VERSION     DAOMAX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_3436_cov_5.009760"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PK742_11460"
     CDS             complement(<1..1535)
                     /locus_tag="PK742_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQU77250.1"
                     /translation="MIRAFIPRLMRICFSIIWLCLLFGSSNAYAQEKEKQEIEIIQAE
                     KLRFTDRNDVKTYLLSGAVILQQGKTTLYCDSAYLFPAWNLASAFGHVHINQSDSVHA
                     YGDSATYDGNARIARLYQNVRLTDRAMILTTDFLTYDMENRIGIYTDGGELRNQEAVL
                     TSDRGYYYSISQDAFFRGDVRLKHPQYRLEADTLQYNTGTEVAWFHGPTTIYNENSTV
                     YCEDGYYETISGIAVFNEHVQMDNPPQELQADSIFYNRETGLGKAFGNIFFRDTSQHI
                     LQYSEVALYDEVNGVITSTERSMAGYILDDDTLFIAGDTIRIVQDSLNGNTMYAYPNV
                     RMYKEDFQGVCDSLYYSDADSIIRLFGMPLLWSDSTQFSGDTIQMQLEANTLREMYFY
                     SNAMIVNEDDSLIYNQIKGRQITGYFQDGDLYKVSVRGNGESIYFGKDEQDRYLGVNQ
                     AVCSDIDLYIRENQFRRISFRELPEATFSPMQQIDPASFRLDGFRWAMDLRPTGRDDL
                     FRET"
     gene            complement(1532..2503)
                     /locus_tag="PK742_11465"
     CDS             complement(1532..2503)
                     /locus_tag="PK742_11465"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQU77251.1"
                     /translation="MKLSNEFRVGVLSIVTIGLLIAGYNFLKGTSLFVKGKQYTIEYS
                     YIPGLKKGDPVQINGYQIGRVTDITLPDAQKGVIEVMVNVTEDISIPKDSRAIIKSAD
                     LLGEKYVDMILGKGNQYYRDGDHITGEIEADLTNQIKEELRPLTEKVQSMIVSVDTAI
                     TVMSSIFTPSFKANFETSVANIKQTLESFNNSARVLDEMLTSQQDRIEGIITDVSAIT
                     HNVDANEKELDQIIDNLKSITDELAAVNWTEISTSLDTALTQFNEIMSAINNSEGSMG
                     MLVNDPELYENLERITQTLDAITKELQQTPGKYVPPLIQIGGRKYSE"
     gene            complement(2559..>3436)
                     /locus_tag="PK742_11470"
     CDS             complement(2559..>3436)
                     /locus_tag="PK742_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQU77252.1"
                     /translation="ERAAIANRNKADLFICIHCNANNSSAPFGTETYVMGLHKTEANL
                     SVAKRENDVILMEDNYSDHYDGFNPNDPSAHIIFSLYQHAYMEQSINFASKCEDQFKG
                     YVGRSSRGVKQAGFLVLWKTSMPAVLIETGFLTNAEEEKFLNSDAGQNKIASGIFRAF
                     SAYKAEMEGTEVSPDLIVQTDSKEEPKKTVQAKGVTPDAYGQGVEFRVQFLAAKELYP
                     SGHASLKGAENMPVTWVQENGWYKYRSGPFSNYSEAQTRLQELKKAGYPDAFLIAHHN
                     GERMDVQEARSLSIR"
BASE COUNT          768 a          858 c          863 g          947 t
ORIGIN      
        1 gtttcccgga aaagatcatc gcgacctgtg ggtctgagat ccatggccca gcgaaatcca
       61 tccaggcgga aagaagcagg atcgatctgc tgcataggag agaaagtggc ttcgggcagc
      121 tcgcggaagc tgatgcgcct gaactggttt tcacgaatgt acagatcgat gtctgagcac
      181 accgcctgat tgacacccag atagcggtct tgttcatctt tgccaaaata gatgctttcg
      241 ccattgcccc gaacactcac tttgtagaga tcaccgtcct ggaaatagcc tgtgatctgt
      301 cgccccttga tctggttgta gatcagacta tcatcttcat tcacgatcat ggcgttcgaa
      361 tagaaataca tctcccgaag ggtatttgct tccagctgca tctggatggt atcgccgctg
      421 aactgggtgc tgtcactcca cagcaggggc atgccaaata atcgaatgat actgtcggca
      481 tctgaatagt acagcgaatc acagacaccc tgaaaatctt ctttgtacat gcgcacattc
      541 gggtaggcat acatggtgtt tccattcagt gaatcctgga ctatccggat ggtatcgccg
      601 gcaataaaaa gggtgtcatc gtccaggatg tatccggcca tcgaccgttc tgtggaagtg
      661 atcacgccgt tgacctcatc atacaatgcc acttcgctgt actgcagaat gtgctggctg
      721 gtatcgcgga agaagatatt gccaaaggct tttcccagcc ctgtttcccg gttgtaaaaa
      781 atgctgtcgg cctgcagttc ctgtggtgga ttgtccatct gcacatgctc attgaataca
      841 gcaatgccgg atatcgtttc atagtaaccg tcttcacaat agaccgtact gttctcgttg
      901 tagatcgttg tcggaccgtg gaaccaggca acctctgtac cggtgttgta ctgcagggta
      961 tctgcttcca gccggtactg cggatgtttc agtctgacat cgccccggaa aaaggcatcc
     1021 tggctgatgc tgtaatagta accgcggtct gaggtcagca ctgcttcctg gttgcgcagt
     1081 tcgccgccat cagtatagat accaatgcgg ttctccatat cataggtcag gaaatcagtg
     1141 gtaagtatca tggctctgtc ggtaagccgc acattctggt acaggcgggc tatcctggca
     1201 ttgccatcgt aggtggcaga atcgccgtag gcatgaactg agtcagactg gttgatgtgc
     1261 acatgaccaa aagctgatgc caggttccag gcgggaaaaa gataggcact gtcgcagtac
     1321 agtgtggtct taccctgctg cagaatgacc gcaccgctca gaaggtaggt cttgacatca
     1381 tttcggtctg taaagcgcag tttttcggcc tggatgatct cgatctcctg cttctctttt
     1441 tcctgtgcat atgcattgct gctgccaaac agcaaacaga gccagatgat agaaaagcag
     1501 atacgcataa ggcggggtat gaacgcccgg atcattcaga atattttctg ccaccgatct
     1561 ggatcagagg cggcacatat ttgccgggtg tctgctgaag ctctttggta atggcatcca
     1621 gggtctgggt gatgcgctcc aggttctcat acagctcagg atcattcacc agcataccca
     1681 ttgaaccttc actgttattg atggcactca tgatctcatt gaactgggtc agtgctgtat
     1741 caagtgaggt ggagatctct gtccagttga ctgcagccag ttcatctgtg atgctcttca
     1801 ggttgtcaat gatctggtcc agctcttttt cattggcatc tacattgtgt gtaatggctg
     1861 aaacatctgt tatgatgcct tcaatacgat cctgctggct ggtgagcatc tcatccagca
     1921 cgcgggcgct gttgttgaag ctttccaggg tctgcttgat gttggcaaca ctggtctcaa
     1981 agttggcttt gaaagacggg gtgaagatgc tgctcatgac cgtaatggca gtatctacac
     2041 tgacgatcat gctctggact ttttcagtaa gtggccgaag ctcttccttg atctggttgg
     2101 tgaggtctgc ttcgatctcg ccggtaatgt ggtcgccatc gcggtaatac tggttgcctt
     2161 tacccaggat catgtccaca tacttttcgc ccagcaggtc ggcacttttg atgatggccc
     2221 tggagtcttt tggaatggaa atatcttctg ttacattcac cattacctca atgacccctt
     2281 tttgcgcatc cggcagggtg atatctgtga cccggcctat ctgataaccg ttgatctgca
     2341 ccggatcgcc ctttttaagg cctggaatgt agctgtattc gatcgtatat tgtttccctt
     2401 taacgaacag gctggtgccc ttcaggaaat tatatccggc gatcagtaaa ccaatggtga
     2461 ctatggacag tacacctacc ctgaattcat tcgacaactt cacggagacc gctctttagc
     2521 aggctaagtt atgggttttt gcagcagggc acaaaacttt agcggatgct cagtgacctg
     2581 gcttcctgta cgtccatgcg ttctccgttg tggtgggcga tgagaaaagc atccggataa
     2641 cctgcttttt tcagttcctg cagccttgtc tgcgcttctg agtagttact gaaaggacca
     2701 ctgcggtact tgtaccagcc gttttcctgc acccaggtaa ccggcatgtt ttcggctcct
     2761 tttaaagagg catgaccgct gggatacagc tcttttgccg ccagaaactg cacacggaat
     2821 tccacaccct gaccataggc gtcaggagta actcctttag cctgaacggt ctttttgggc
     2881 tcttccttgc tatcggtttg tacgatcagg tcagggctga cctcggtgcc ttccatctca
     2941 gccttgtaag cactgaaggc acggaagata ccggatgcga tcttgttctg accggcatct
     3001 gagttcagga atttctcttc ttcagcgttt gtcaggaaac ccgtttcaat gagtaccgcc
     3061 ggcatactgg tcttccacaa taccagaaat cctgcctgct tcaccccgcg gctggacctg
     3121 cccacatagc ccttgaactg gtcttcgcat ttgctggcaa aattgatgct ctgctccatg
     3181 tacgcatgct ggtacagaga gaatatgata tgtgcagaag gatcgttcgg attgaagccg
     3241 tcgtaatgat cagaatagtt gtcttccatc aggatgacat cgttctctct tttggcaaca
     3301 gacaggttgg cttccgtctt gtgcagaccc atcacatagg tctctgtgcc gaatggtgca
     3361 ctggaattat tggcgttaca gtgaatacag atgaacagat cagccttgtt ccggttagca
     3421 atggctgccc gttcat
//
LOCUS       DAOMAX010000275         3435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5459_length_3435_cov_4.398225, whole genome shotgun sequence.
ACCESSION   DAOMAX010000275 DAOMAX010000000
VERSION     DAOMAX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5459_length_3435_cov_4.398225"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PK742_11475"
     CDS             <1..546
                     /locus_tag="PK742_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77253.1"
                     /translation="DDSSGVFLCDAPDGLMADAAATSATLSWNMVDGAFMYRIKVEDG
                     SGNPVDFLTHGTTGSTSFDVSGLEPSSNYKFKVRTLCVGDHSGWSSWKPFTTAPLRMA
                     APVMESIYPNPAADQVNLVLLPAEVNAQLQVIDMTGRIMFAEVIPANTTSFQIPTAGW
                     PEGMYRILLQNNESKPLMIAR"
     gene            641..2650
                     /locus_tag="PK742_11480"
     CDS             641..2650
                     /locus_tag="PK742_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HQU77254.1"
                     /translation="MQTTSGISHPAFSYDRGNLSHEQLTDLYSRLLAPRMIEEKMLNL
                     LRQGRISKWFSGIGQEAISVAAAWVLEADEYILPMHRNLGVFTTRDVPFGKLFKQWQG
                     GYNGFSKGRERSFHFGTNDYHIVGMISHLGPQLTIADGIALAAKLKQENKVTAVFTGE
                     GGTSEGDFHEALNVAAVWDLPVIFIIENNGYGLSTPTGEQYRCENLADRGVGYGMRAL
                     TVDGNNILEMVHTLTELREWCIREQKPVLLECRTFRMRGHEEASGVKYVPPTLLEEWA
                     KKDPVRNFEAWLRNEGLLDDTAAEHIRTSISVLIDKGLQEGFAEPPLEPTVEAELADV
                     YKEHTFVQTSPRVSAATEKRMVDAISDGLWQAMKKHENLVLMGQDIAEYGGVFKITER
                     FVDEFGHGRVRNTPICESAILGAAVGLSIKGMKSMVELQFADFVTSGFNQVVNNIAKM
                     HYRWGQHVDTVLRMPTGAGVGAGPFHSQSNEAWFFHTPGLKIVYPSTAEDAKGLLLSA
                     FEDPNPVMFFEHKAMYRSISGAVPDDYYTIPIGKARLVQEGEELSVITYGMGVHWATR
                     AASQLGIDARILDLRSLVPLDYEAISETVQATGKVLVLHEDTLTGGIGAEISAWINEH
                     LFEYLDAPVMRCASLNTPVPFAKPLEEQYLATARLQKVMLDLQSY"
     gene            2826..3146
                     /locus_tag="PK742_11485"
     CDS             2826..3146
                     /locus_tag="PK742_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77255.1"
                     /translation="MRTKKNDQVIHILRSSLHRFGAWLHMVHVQDVILYNREGLPLVK
                     CSISTTPFLRTDEPAHQKVDLENGSYGYCYFYQDAEDVPSNICVFGKDDKGSSFSFRS
                     IISH"
     gene            3172..>3435
                     /locus_tag="PK742_11490"
     CDS             3172..>3435
                     /locus_tag="PK742_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77256.1"
                     /translation="MNVISKAGLLAFLLGTLVACSKSDLSEMLSATDPTTLDSGISGG
                     TDDPAGDDSGTGGGTDDPSGDDHGSGGGGADDPSGDDSGDDDGD"
BASE COUNT          871 a          825 c          896 g          843 t
ORIGIN      
        1 gatgattcat caggcgtttt cctgtgtgat gcacctgacg gactgatggc tgatgcggct
       61 gctacctctg ccaccctgag ctggaatatg gtagatggtg ctttcatgta tcgtatcaaa
      121 gtggaagacg gatcaggcaa tccggtagat tttctgactc atggaacaac cggcagcacc
      181 agttttgatg tgagcggact ggagccatcc agtaactata aattcaaagt gcgtaccctt
      241 tgcgtgggcg accacagcgg atggtcttcc tggaaaccat tcactactgc accgctccgt
      301 atggctgctc ctgtaatgga aagcatatac ccgaatcctg ctgcagatca ggtcaatctc
      361 gtactgctgc cggcggaagt gaatgcccag ctgcaagtga tcgatatgac cggacgcatt
      421 atgttcgctg aagtgatccc ggctaatacc acttccttcc aaatcccaac tgccggatgg
      481 cctgaaggta tgtatcgtat tctgctgcag aacaatgaga gcaagccctt aatgatagcc
      541 agataaactg tttttgtggg taacaggagg ccacgacgaa agtcgtggct ttttttttac
      601 ctcgtttctt ccgcatctgc aaccgtccta cctttgcacc atgcaaacca cttcaggtat
      661 atctcaccct gctttttcct atgaccgcgg caatctgagc catgaacaac tcacagacct
      721 gtacagccga ctgctggccc cgcgaatgat cgaagagaaa atgctcaatc tgttgcgtca
      781 gggcaggatc agcaagtggt tttcgggtat cggacaggag gccatctccg tagctgccgc
      841 ctgggtgctc gaagcagatg aatacatctt gcccatgcac cgaaacctgg gtgtcttcac
      901 cacaagagat gtacccttcg gcaaactgtt caaacaatgg cagggtggtt acaacgggtt
      961 ctccaaaggc agagaacgca gcttccactt cggcaccaat gattaccata tcgttggaat
     1021 gatctcacat ctcggtccgc agctcactat cgctgacggt atcgcactgg cagcaaagct
     1081 gaaacaggaa aacaaggtga ccgctgtgtt taccggcgaa ggaggaacca gtgaaggaga
     1141 ttttcatgaa gcactcaacg tagctgcagt atgggatctg cccgtcatct tcatcattga
     1201 gaacaacggt tacggcctgt ccaccccaac gggtgaacaa taccgttgtg aaaacctggc
     1261 agatcggggt gtgggttatg gcatgcgtgc gctgaccgta gatggcaata atatcctgga
     1321 gatggtgcat acgctgaccg aactccggga gtggtgcatc cgtgagcaaa aacctgtgtt
     1381 gctcgaatgc cgaaccttcc gcatgcgtgg tcatgaagaa gccagtggtg tgaagtatgt
     1441 accacccact ttgctggaag aatgggccaa aaaagacccg gtcaggaatt ttgaagcctg
     1501 gttgcgcaat gaaggattgc tggatgatac cgctgctgaa cacatccgta cttctatttc
     1561 tgttttgata gataaaggcc tgcaggaagg atttgcagaa ccgccccttg agccgaccgt
     1621 agaagcagag ctggcagatg tatataaaga acacacattc gttcaaacat ctccaagggt
     1681 aagtgctgct actgagaaac gtatggttga cgccatatca gacggtttgt ggcaagccat
     1741 gaagaaacat gagaacctgg tactgatggg gcaggacatt gctgaatatg gcggtgtatt
     1801 caagatcaca gaacgcttcg tcgatgaatt cggacacgga agggtgcgca atacgcctat
     1861 ctgtgaatct gctattctgg gtgctgctgt cggcctgagc atcaaaggca tgaagagtat
     1921 ggtggaactg cagtttgcag atttcgttac ttcgggtttt aaccaggtgg tcaacaacat
     1981 cgccaagatg cactatcgct gggggcagca tgtggacacg gtgcttcgca tgcctaccgg
     2041 tgcaggggtg ggtgccggac cttttcacag ccagagcaat gaggcctggt tctttcatac
     2101 acccgggctg aagatcgtgt atccatccac tgcagaagat gcaaaaggat tattgctgag
     2161 tgcatttgaa gatcccaatc ccgtcatgtt ctttgaacac aaagccatgt accggagtat
     2221 cagtggtgct gtacctgatg actattatac cattccaata ggcaaagcca ggctggtgca
     2281 ggaaggggaa gagttgtctg tgatcaccta tggcatgggc gtacactggg caacccgggc
     2341 agcttctcaa ttgggtattg atgcacgcat cctggatctt cgaagcctgg tacctctgga
     2401 ctatgaagcc atctctgaaa cagtacaggc aaccggaaaa gtgctcgtgt tgcatgaaga
     2461 tacgctgacg ggtggcatcg gtgcagagat ctctgcatgg atcaatgaac acctgttcga
     2521 ataccttgac gcacctgtca tgcgctgtgc ttcgctgaat acaccagtgc cgtttgcaaa
     2581 accactggaa gaacagtacc tggcaaccgc ccggttgcaa aaagtgatgc tggatctgca
     2641 gagttattag gccgtttttc cggaactgcc ggattcttgt ccccttctga accacccggg
     2701 aaccgctgtt ttcagctgat ttgatcagta tatgcagata actgccggcc aaatccggaa
     2761 tatatggatg aaaatggcaa taagatgttc ttttttgcct gtttgttccg gtgcattttt
     2821 ggaacatgag aacgaaaaag aacgatcagg tcatccatat cctgaggtca tctttgcacc
     2881 gcttcggtgc atggcttcat atggtgcatg tacaggatgt catactctat aaccgcgaag
     2941 ggcttccttt ggttaagtgt agcatttcta caactccgtt ccttcgtact gatgaaccgg
     3001 cacatcaaaa ggtcgatctg gaaaacggaa gttacgggta ctgctacttc tatcaggatg
     3061 ctgaagatgt tccaagcaat atctgtgttt tcggaaaaga tgataaaggt tcttccttca
     3121 gtttccgctc tattatttca cattaaccaa aatcaataca acatgcgaat catgaatgta
     3181 atttcaaaag ccggtctgct ggctttcctg ctcggcacac tggtggcctg cagcaaatct
     3241 gacctttcag aaatgttatc tgcaaccgat ccaactacac tggacagcgg cattagtgga
     3301 ggtactgatg atccggccgg cgatgacagc ggaaccggcg gtggaacaga tgatccatca
     3361 ggtgatgacc acggatccgg tggcggtggt gccgatgacc cctccggcga tgatagtggt
     3421 gatgacgatg gcgat
//
LOCUS       DAOMAX010000276         3404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5539_length_3404_cov_4.054942, whole genome shotgun sequence.
ACCESSION   DAOMAX010000276 DAOMAX010000000
VERSION     DAOMAX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5539_length_3404_cov_4.054942"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PK742_11495"
     CDS             complement(join(<1..856,856..1117))
                     /locus_tag="PK742_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369065.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQU77257.1"
                     /translation="MKKSRFSETQIVGILKQHEQGLKVADICRQHGISSATFFNWKSK
                     YGGMQASDIKRLRELEEENRKLKQMYADLALDNRILKDIIEKKPLRPCHKKELVTGIS
                     SEYCVSIGRACRIINLPRSMYYYESKRDDTAVIDELSKLAEQLPTNGFPEYFGRIRRV
                     HQWNHKRVRRVYRKMGLHIRCKHKRRLPQRIQQPLQQQPSLNKVWSMDFMQDSLTSGR
                     KFRVLNVIDDFNRQGLAIRIATSFPGQEVTRILTQLIEEYGKPKRIRTDNGPEFISRS
                     VQEFMQTNEIKPQFIQPGKPTQNAYIERFNRSFRTVVLNAYLFDDINQVRALSGTWLD
                     DYNRNHPHKSLFGLSPIEFREDVNSGKLVFNDQLEQFTT"
     gene            1294..1875
                     /locus_tag="PK742_11500"
     CDS             1294..1875
                     /locus_tag="PK742_11500"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HQU77258.1"
                     /translation="MNRLEEFRSHLKQGKVYRREDLIRWTASVDRHLAILVQEGFLTK
                     LSQGLYYVPKESVFGKVPPEEEQLVSSFLKDKRFLIFSPNAYNTLGVGTTQLYNLTVV
                     YNKKRHGIFKLGNREFDFRRKYDFPARLTPEFLFVDLINNLGDLAEDSNDILNNALAK
                     YSQVQSPKWINAVNKYGNVKTKKLLLKSVESIA"
     gene            1875..2828
                     /locus_tag="PK742_11505"
     CDS             1875..2828
                     /locus_tag="PK742_11505"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQU77259.1"
                     /translation="MPRYLHDHPSFIDLLNIVAEEKGVEPGLVEKDYWIMHVLYGLKE
                     QGFEFELKGGTSLSKGYQIIERFSEDIYIHMKPPAWFEINENPKNCNPRNVESRKRYY
                     DWLASHIRIDGIVSVERDTLFDETECYRSGGIRLHYDARTNSIDGVKEGVLLEAGFDI
                     TSPNSPILISSWAIEKALSQQVMIIDNRAKEILCYHPGFTLVEKLQTIATKYRHEIVE
                     GGTAKVNFMRQYYDVYCLLSNETVLAFIGSEPYNEHKRSRFPEADLDIPVSQNEAFLL
                     TSEEQRAVFKARYHATAALYYNGQPPFNDLLERIREHIHLL"
     gene            complement(2906..>3404)
                     /locus_tag="PK742_11510"
     CDS             complement(2906..>3404)
                     /locus_tag="PK742_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQU77260.1"
                     /translation="DQLQQAKKIRGPRAKASDPDALKEGSVVMLLDGEERGTIESIRN
                     DKAVVVFGQIRTTVPLKELRIADATLPTPELKTKVKVQLEEETVFELDVRGMDQLTTI
                     MEVERFLDGALVRNYRHVRIVHGIGTGVLRDTVHQVLRQHPTIQQFKLAGRDAGGNGA
                     TEVEL"
BASE COUNT          936 a          692 c          742 g         1034 t
ORIGIN      
        1 gtggtaaatt gctccaattg atcattaaac accaattttc cactgttcac atcctcccta
       61 aattctatag gacttagacc gaagagcgat ttgtgcggat gatttctgtt gtaatcatcc
      121 agccacgtac ctgaaagagc tcttacctgg ttgatatcat caaatagata tgcgttcaat
      181 actaccgtcc tgaagcttct attgaaacgt tcaatgtaag cattttgtgt gggcttgccg
      241 ggttgaatga attggggttt aatctcattt gtttgcatga actcctgtac tgatctgctg
      301 ataaattcgg gtccgttgtc tgttcttatt cttttgggct tgccatattc ctcaataagt
      361 tgagtaagta tacgagtcac ttcttgtcct gggaaagagg tggctattct gattgcaagt
      421 ccttgtctgt taaaatcatc tatcacatta agcactctga attttcttcc gctggtaagt
      481 gaatcttgca taaagtccat actccaaact ttgttcagtg atggctgttg ttgaagcggc
      541 tgctgtattc tttgcggtaa cctacgtttg tgcttacaac gaatatgcaa ccccatcttc
      601 ctgtaaaccc tccttacccg tttgtgattc cattgatgaa ctcttcttat tctgccgaaa
      661 tactcaggaa atccatttgt tggcagctgt tcagcaagtt tactcagttc atcgatcact
      721 gcggtgtcat cacgcttact ttcatagtaa tacatgctcc ggggaagatt aatgattctg
      781 catgccctgc caatcgaaac gcagtattca ctggaaattc ctgttacgag ttccttctta
      841 tggcagggcc ttaaagcttt ttttcaataa tatcttttag gattctattg tccagtgcca
      901 ggtcagcata catctgcttt aactttcggt tctcttcttc caactcccga agccgcttaa
      961 tgtcagaggc ctgcatgccc ccatacttcg atttccagtt gaaaaaggtc gcactcgaaa
     1021 tgccatgttg ccgacaaatg tcggctactt tcaagccctg ctcatgttgt ttgagaatcc
     1081 ccactatctg ggtctcacta aatctgcttt ttttcattgt ttgacaattt aaagttagat
     1141 atactctaat tttaaactgt ccgaatttta gggatactta cagatactta caaaaggttg
     1201 aaaaacaata aaaaaagagt ataaaatgca tgaaaaatga aataaaaaac ttacattagg
     1261 aattattttt gctattttcg tgtataaaat tatatgaata gactcgagga atttaggtct
     1321 catttaaagc aggggaaggt gtacaggcgg gaagatctga tcagatggac tgcatctgtt
     1381 gatagacacc tggctatact ggtgcaggaa ggttttttga caaagctttc ccagggattg
     1441 tactatgttc ccaaagagtc tgtttttgga aaagtccctc cggaagagga acagctagtg
     1501 agcagcttct taaaagataa gaggtttttg atcttttcac ccaatgccta taacaccctt
     1561 ggtgtgggta ccactcaatt gtataatctg actgtggtgt acaacaaaaa acgtcatggg
     1621 atatttaaac tcgggaacag agagttcgat tttcgtcgca agtatgattt tcccgccagg
     1681 cttacacctg aatttctgtt tgtagatctg atcaataacc tcggagatct ggctgaagat
     1741 agcaatgaca ttttaaacaa tgcactggcc aaatactctc aggtgcaatc tccaaaatgg
     1801 ataaatgctg ttaataaata tggcaatgta aaaactaaga agcttttact taaatcggtg
     1861 gaaagcatcg cgtgatgcca aggtaccttc atgatcatcc ctcctttatt gacctactga
     1921 acatagtagc agaagaaaag ggagttgaac caggtttggt agaaaaggat tactggatca
     1981 tgcatgtgtt gtatggtctc aaagaacaag gatttgaatt tgaattaaaa ggaggaactt
     2041 ctttatcaaa gggttatcag atcattgaaa gattttctga ggatatatat attcacatga
     2101 aaccgcctgc atggtttgaa ataaatgaaa atccaaagaa ttgcaatccc cgaaatgttg
     2161 agagtagaaa aaggtactat gactggttgg caagccacat cagaatagat ggaattgtta
     2221 gtgtggagcg agatacctta ttcgatgaaa cggaatgtta tcgaagtgga gggattagat
     2281 tacactatga cgcaagaacg aacagtattg atggggttaa agagggtgtg cttcttgaag
     2341 ctgggtttga tataaccagt ccaaatagcc ccatacttat aagttcatgg gctattgaga
     2401 aggctttatc tcaacaagta atgataattg acaatcgtgc taaagaaatt ttatgctatc
     2461 atccagggtt tactttggtc gaaaaattgc aaacaattgc aactaagtat cggcatgaga
     2521 tagtggaagg tggaactgcg aaggttaact tcatgaggca gtactacgat gtttattgtc
     2581 tgctcagtaa tgaaacagtc ctggcattta ttggaagtga accgtataac gaacacaaaa
     2641 gatcaagatt ccctgaggca gatcttgata ttcctgtttc tcaaaacgag gcttttttgt
     2701 tgacttctga agaacaaagg gccgttttta aagccagata tcatgctact gctgctttgt
     2761 attacaatgg tcagccaccc tttaatgatc tgttggaaag gatcagggag catattcatc
     2821 tgttgtagaa tacatgctga ttggtcaggt tatcaaagga ttgcgttcag catctgatgt
     2881 tgttatataa agatcatgtg tatcctcaca actccacctc cgtcgctcca ttaccccccg
     2941 catctctgcc ggctaatttg aattgctgaa tggtagggtg ctggcgcaac acctggtgca
     3001 ccgtatctcg taagacacct gtaccaatgc cgtgcacgat gcgcacgtgg cggtagttgc
     3061 gcacaagagc gccgtccagg aagcgctcta cctccatgat ggtggtgagc tgatccatgc
     3121 cgcggacatc cagctcaaac acggtctctt cttctaattg taccttgacc tttgttttga
     3181 gttcgggggt gggcagggtg gcatcggcta tgcgtagttc cttgagggga acagtggtcc
     3241 tgatctgccc gaacaccacc acggccttgt cgttgcggat gctttcaatg gtgcctcgtt
     3301 cttctccgtc taacagcatg accactgaac cttctttcag ggcatccgga tcgctggctt
     3361 tggcccgggg accgcggatc ttcttggcct gctgcagctg gtct
//
LOCUS       DAOMAX010000277         3403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5542_length_3403_cov_4.275687, whole genome shotgun sequence.
ACCESSION   DAOMAX010000277 DAOMAX010000000
VERSION     DAOMAX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5542_length_3403_cov_4.275687"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK742_11515"
     CDS             complement(<1..411)
                     /locus_tag="PK742_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQU77261.1"
                     /translation="MKSVTTARVIGLLTGPALCLIICLLPGDLVSPVADKVLGVAAWI
                     VSWWITEAVSISVTALLPLLLFPLLGISTMRDVSVHYGDPVIFLFFGGFIIALAMEKV
                     HLHKRIALSIIRLTGTSPNRVILGFMIATGLLSMW"
     gene            complement(445..1278)
                     /locus_tag="PK742_11520"
     CDS             complement(445..1278)
                     /locus_tag="PK742_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQU77262.1"
                     /translation="MQLLNRLVCLSVLWIPSLRGSAQTDSIDRQHLSIGEVHTIHSDI
                     LQEDRILNIYTPEGFDTLQNLPVIYLLDGSINEDFIHVCGLVQFFHLMYGMPPCIVVG
                     IANVDRKRDFTFHTDLAELTDKYPTVGHSEPFIRFLSEELQPYVREQFNTGDTNMLIG
                     QSLGGLLATEILLKQPDLFTHYFIVSPSLWWDNESLQRRFAELSTTLQNKHLFIFISV
                     GKDEDRIMVREARTLHKQLRSLHGNKLDLYYHPMSGENHATVLHSSLYEGLKILFPYQ
                     E"
     gene            complement(1323..1739)
                     /locus_tag="PK742_11525"
     CDS             complement(1323..1739)
                     /locus_tag="PK742_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77263.1"
                     /translation="MRCPKIDQAILCFLLVAGSVMISCHTCSAQSPDRKQVAGRYVHE
                     TGQPARTISGEDGSEASIQAIIYSRSVLHLRRSGTSKECHTPLQGDLPYCYKSRWYLQ
                     DDSVVIRHGDTITTYLWSENTLTGTNSDQSWHRTSE"
     gene            complement(1720..1956)
                     /locus_tag="PK742_11530"
     CDS             complement(1720..1956)
                     /locus_tag="PK742_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77264.1"
                     /translation="MKTLTCKQLGGACDHAFHAETFEDMAAQSQQHGKEMWQQGDADH
                     MKAMQDMQVLMTDPKALQAWMEEKQRLFDALPED"
     gene            complement(1963..2646)
                     /locus_tag="PK742_11535"
     CDS             complement(1963..2646)
                     /locus_tag="PK742_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77265.1"
                     /translation="MKNKDKAFDYISRWTFEDAGIAEKLSADPGIDEEDKAELTRMFS
                     VFQTLLKKSSQYDAMSQEENKRVSIEVNNMAVENMRNTYGTIMSIKTSLQDVIKDAKR
                     AYTYVLWMYILAFLMGVGLIVLSVVFAIQGKDILAIAFGGIGLIDLVTYFIYKPPLEI
                     QNSRSNLAQLMIIITNWFADLMNLNTYMSNRNAIITLEEIDQISEKQNQNTAKTIDLI
                     EKYSESKGK"
     gene            complement(2820..3266)
                     /locus_tag="PK742_11540"
     CDS             complement(2820..3266)
                     /locus_tag="PK742_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77266.1"
                     /translation="MVKLQPLNVVRKIRIIPYSKALYFLLLLSAIYSCDPARVLIVRS
                     AYPEQASVTIYADQAFLPSIYSADTGQVRITVPDGAGNSELIFMYGLGGWANDSLLYQ
                     MSGQVDSICFTGIQGNHTLYLQDEIYDYLRLSRSGFASRKLTITAR"
BASE COUNT          893 a          765 c          827 g          918 t
ORIGIN      
        1 ccacatgctt aacaatccgg tggcgatcat gaagcccaga atgacacggt tcggactggt
       61 gcctgttagc cggatgatgg acaacgcaat gcgtttgtgc aggtgcactt tttccatggc
      121 cagggcgatg atgaatccgc cgaagaacag gaagatgact gggtcgccgt agtgcacaga
      181 aacatcccgc atggtgctga tgcccagaag cggaaataac agcagcggaa gcagagcggt
      241 cacactgatg gatactgctt cagtgatcca ccaggaaacg atccaggcgg ccacacccag
      301 taccttatcg gcaacgggcg aaacgaggtc tccgggtagc agacatatga tcaggcacag
      361 ggcaggtccg gtaagcagac cgatcactct ggctgtggtc actgatttca cgcaataatg
      421 ttaccaaaaa aatgtgcgat aactttattc ctggtaggga aaaaggatct tcagaccttc
      481 atataaactg ctgtgtagta cagttgcatg gttctcgccg gacatcggat gatagtacaa
      541 gtcaagtttg ttgccatgta gtgatcgcaa ttgtttgtgc aaggtacgag cctctctgac
      601 catgatgcgg tcttcgtctt ttccaactga gataaaaatg aacaggtgct tgttctgtag
      661 tgtggttgat aattctgcaa atctcctttg caggctttcg ttgtcccacc acagactcgg
      721 gcttacaatg aaatagtggg taaacagatc cggttgtttt agcaggatct ctgtagccag
      781 taaaccacct aatgactggc ctattagcat gttcgtgtcg ccggtattga attgctctct
      841 gacatacggc tgcaactctt cagacaggaa tcgaatgaat ggttctgaat gaccaacggt
      901 agggtattta tcagtcagtt ctgccaggtc ggtatgaaaa gtaaaatcac gtttgcggtc
      961 cacattggcg atacctacca cgatgcaggg gggcatgccg tacataaggt gaaagaactg
     1021 aaccaggcca catacatgaa tgaaatcttc atttatgctg ccatcaagca ggtagatgac
     1081 cggcaggttc tggagggtgt cgaatccctc gggggtatat atgttcagga tgcggtcttc
     1141 ctgcaggata tcagagtgga tggtatgtac ctctcctata gaaaggtgct gcctgtcaat
     1201 ggaatcagtt tgtgctgagc ctctcagtga aggtatccat aaaacagaaa ggcacacgag
     1261 acggttaagc aattgcattg ctcaaagata ggagggaaag acgggccgga gataaaaaaa
     1321 gatcactctg aagttcgatg ccaggactga tcgctgtttg taccggtcaa tgtgttctct
     1381 gaccacaaat aggtcgtgat cgtatcgcca tgtctgatga caacagaatc atcctgcagg
     1441 taccacctgc ttttgtaaca gtaaggcaga tcaccctgta aaggtgtatg acattcttta
     1501 cttgttccgg atctgcgaag gtgaagtaca cttcggctgt atatgattgc ctgaatggat
     1561 gcttcagatc cgtcttctcc ggagatggtt ctggcaggtt ggccggtctc gtgtacatag
     1621 cgcccggcta cctgttttct gtcgggcgac tgagcagaac atgtatggca gctgatcata
     1681 acggagccgg caacaagaag aaagcataaa attgcctgat caatcttcgg gcagcgcatc
     1741 gaacagtctt tgtttttctt ccatccatgc ctgcaaggct ttcgggtcgg tcatcagtac
     1801 ctgcatgtcc tgcatggctt tcatatgatc ggcatcacct tgctgccaca tctctttgcc
     1861 atgttgctgg ctttgtgcag ccatgtcttc aaaggtctct gcgtgaaagg catgatcgca
     1921 tgccccaccc agttgcttac aggtcaatgt tttcatacat acttatttgc cttttgactc
     1981 gctgtacttt tctatcagat caatggtctt ggcagtattc tgattttgtt tttcagaaat
     2041 ctggtctatc tcttccagcg taataatggc atttctgttg ctcatgtagg tattgagatt
     2101 catgagatct gcaaaccagt ttgtgatgat gatcatcaac tgagccagat tgctgcggct
     2161 gttctggatc tcgagtggtg gtttatagat aaagtaggtc accagatcta tcagtcctat
     2221 gccgccaaag gcaatggcca gaatgtcttt accctgaata gcaaatacca cagacaagac
     2281 gatcagacca acccccatta gaaaggcaag tatatacatc caaagtacgt aagtgtaggc
     2341 tcttttagca tctttaatga catcctgcag agaagtcttt atgctcatga tggtgccata
     2401 ggtgttgcgc atattttcaa cagccatatt gttcacttcg atacttaccc gcttattctc
     2461 ctcctgactc atagcatcgt actgggatga ttttttcagc agggtctgaa acacactgaa
     2521 cattcgtgtc aattcagcct tatcttcttc atcaataccg ggatcggcag ataatttttc
     2581 agcgatgcca gcatcttcaa aagtccacct ggaaatgtag tcgaatgctt tatccttgtt
     2641 tttcataagc gtagaaatat gaatagatac aaactaaaga tacatggtgt tcatgaaatg
     2701 aaaagcgccg gggtgcctta atttctattt ccgcataata aaaacggtta gggaccattg
     2761 gagtttccga tgctgttcag gataccaata gaagctgcac agggattatt cagatagtat
     2821 taccgtgcgg tgatggtcag tttccggctg gcgaatcccg acctgcttag cctgagataa
     2881 tcgtagatct catcctgtaa gtagagggtg tggttgccct gtatgcctgt gaaacagatc
     2941 gaatctacct ggccgctcat ctgatacaac aggctgtcat tggcccagcc acctaatccg
     3001 tacataaaga tcagttcgct gttgccggca ccgtctggta cggtgatacg gacctgaccg
     3061 gtatcagcac tatagataga gggcagaaat gcctggtcgg catagatggt cacggatgcc
     3121 tgttccgggt atgcagaacg aacgatgagc acccgggccg gatcgcagga gtagattgct
     3181 gataacaaca acaggaaata aagtgcttta gaataaggaa taatcctgat ctttctaacg
     3241 acattcaatg gctggagttt taccattttc ctattaatgt cacttgaaaa ttaggataca
     3301 gatactcaaa cgccatgacc acccaggatg gtaagttctt gtccacctgt tggcatacca
     3361 tccaatagtg gcatagcctg ttgaatgagc tgccgtactt cag
//
LOCUS       DAOMAX010000278         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5718_length_3344_cov_3.990271, whole genome shotgun sequence.
ACCESSION   DAOMAX010000278 DAOMAX010000000
VERSION     DAOMAX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_3344_cov_3.990271"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2169)
                     /gene="ligA"
                     /locus_tag="PK742_11545"
     CDS             complement(61..2169)
                     /gene="ligA"
                     /locus_tag="PK742_11545"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQU77267.1"
                     /translation="MKYAASQQQELSALTRKLLQEGEAVPGKESEATQTASSLRQVII
                     FHDWKYYVQADNIITDFEYDLLFRRLKAIEAAYPSLVTPDSPTQRVARDLTEDFPSVT
                     HSVPMLSLDNSYNIDDLIDWDARVKGFVGDEEVIYCVEPKFDGGSIALLYENDILVRA
                     ATRGDGTTGEEITNNARRMRSVPLRADFSSIGLQKVELRGEVVINKQTFADINRKREE
                     EGLNILQNPRNSAAGALRVKDAEEVSRRGLEAFIYAIGFAVDVKGENQLGDTLQKHFD
                     NIEWLGKLGFKVPVQEKKRCKGIQEVMDFINEWEARRDDFAYEIDGMVIKVDDLKQQQ
                     LCGFTAHHPRWAIAFKFKAREAATELEGVEFQVGRTGAITPVAKVKPVYIGGVTVSSI
                     SLHNLDIIREKDIRLGDTVIVQRAGDVIPYIDRVVLEKRSGKETSIDFPDGCPSCGAP
                     IHRPEGEAVFRCSNIECPAQAEERLIHFVSKGAMDMDGLGRETVIDFFRRGWLKSIPD
                     IYRLPYEEILSLEGWKEKSVNKLRESIEASKQQPLWRLVNGFGIRHVGTQTAKDLVRH
                     LDKLTDLFDYDAEKLTEIEGIGQIVASSITEFFHNPGNRHMITELETLGLNLVQEKQA
                     GGGKLEGKTFLFTGSLTRFTRDQAKEMVEANGGRILSSVSANLHYLVAGEKAGSKLKK
                     AEAIPSIQVIDEEQFLQMIA"
     gene            complement(2499..2738)
                     /locus_tag="PK742_11550"
     CDS             complement(2499..2738)
                     /locus_tag="PK742_11550"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQU77268.1"
                     /translation="MWIEENNQLRRTFVFEDFTEAFSFMTRVAFLAEKHNHHPNWCNV
                     YNKVEIHLCTHDAGDTITDRDRKLAAAIDKLHQEG"
     gene            complement(2751..>3344)
                     /locus_tag="PK742_11555"
     CDS             complement(2751..>3344)
                     /locus_tag="PK742_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQU77269.1"
                     /translation="TQMMESMIEQARPTRAEVTDVANAVMDGADAVMLSGETAMGQHP
                     ELVVKTMSRIINKAEKEDIIYNRRHVADPKSRTFLSDAVCYNSCNMADEVGARAIMGM
                     TKTGYTAFMVSSSRPRAAIYIFTNNQEIVNTLNLLWGVKAFYYTRLKGTYETFMDVQE
                     VLKGKKLLQKGDIVLNLGTMPVTQPSRTNVLRISIIE"
BASE COUNT          687 a          894 c          866 g          897 t
ORIGIN      
        1 cagccagctg ttcgttccgc tcttttgcgg ttggttctca tccattctgt acatagtata
       61 tcacgctatc atttgtaaga attgttcttc atctatgacc tggatagagg gaatggcttc
      121 ggccttcttc agcttgctgc ctgctttctc tcctgccacc aaatagtgca ggttggcact
      181 cacgctgctc aggatcctgc caccgttggc ttccaccatc tccttggcct gatcgcgggt
      241 gaaacgggtc agactgcccg tgaacaggaa ggtcttgcct tccagtttgc cacctcctgc
      301 ctgtttctcc tgtacgagat tcaaccccag tgtttccagc tctgtgatca tatggcggtt
      361 gccgggatta tggaagaact cggtgatgga agatgccacg atctgtccga tcccctctat
      421 ctcagtgagc ttttctgcat cgtaatcgaa gagatcggtc agcttgtcga gatgccgcac
      481 aaggtccttt gctgtttgcg tgcccacgtg ccggatgccg aaaccattga cgagtctcca
      541 tagcggttgc tgcttgctgg cctctatgct ctcccgaagc ttgttcacgc tcttctcttt
      601 ccatccttcc agtgacagga tctcttcata gggcaggcga tagatgtcgg ggatggactt
      661 cagccagccg cgacggaaaa aatcaatgac ggtttcacga cccagtccgt ccatatccat
      721 cgcacctttg gagacgaaat ggataaggcg ctcttctgcc tgcgccggac attcgatgtt
      781 gctgcagcgg aacacagctt ctccttccgg acgatggatg ggtgcgccac aactcgggca
      841 accatcagga aagtcgatgg atgtttcttt accgctgcgt ttttccagta ctactctgtc
      901 gatgtaagga atgacatcgc cggctctttg cacgatgacc gtatcgccca ggcgtatgtc
      961 tttctctctg atgatatcga ggttgtggag ggagatgctg ctgaccgtca ccccgccgat
     1021 gtacaccggt ttcaccttgg ccacaggtgt gatggcaccg gttcgtccca cctggaactc
     1081 cactccttcc aactcggtgg cggcctctct tgctttgaat ttgaaggcga tggcccagcg
     1141 gggatggtgg gcagtgaaac cacacagctg ctgttgtttc aggtcgtcca ccttgatgac
     1201 catgccgtct atctcgtagg caaagtcgtc gcgacgggct tcccattcgt tgatgaagtc
     1261 catcacttcc tgtatgccct tgcagcgttt cttctcctgt acaggtactt tgaaaccgag
     1321 cttacccagc cattcgatgt tgtcgaaatg tttctgcagt gtatccccca gctggttctc
     1381 tcctttcaca tccaccgcaa acccgatagc atagatgaaa gcctcgagtc cgcggcgact
     1441 cacctcttcc gcatctttga cacgcagggc accggcagca gagtttctcg gattctgcag
     1501 gatgttcagg ccctcttctt cccgtttgcg gttgatatcg gcaaaggtct gtttgttgat
     1561 gaccacttcg ccccgcaact ccaccttttg caatccgatg gaggagaaat cggcacgaag
     1621 cggtaccgaa cgcatgcgtc tggcgttgtt ggtgatctct tcgccggtgg tgccatctcc
     1681 acgggtggca gctctcacca ggatatcgtt ttcatacaac agagcaatgc ttcctccgtc
     1741 aaacttgggt tccacgcagt agatcacttc ttcatcgccc acaaatcctt tgacccttgc
     1801 atcccagtcg atgaggtcgt ctatgttata ggagttgtcc agcgacaaca tcggaacact
     1861 gtgggtgacg gatggaaagt cttccgtgag atcgcgggcc acacgctggg tgggtgagtc
     1921 gggtgtgacc aatgatggat aggccgcttc gatggccttc agtcggcgga agagcagatc
     1981 gtattcaaag tcggtgatga tgttatccgc ctgcacataa tatttccaat cgtggaagat
     2041 gatgacctgc cggagggagg atgctgtttg tgtagcttct gattcctttc ccggaacggc
     2101 ttctccttcc tgcagcagct ttctggtcaa tgctgacaat tcctgttgct gtgaagcggc
     2161 atatttcatg ggtgaaaatt agggaaagaa tacagaacgc agataacaaa gacgggaacc
     2221 acagagggca tggagttgca cagagggaac actttgaata actgaccaaa ctcaaaatag
     2281 aatgtctctc cgggcttgac ccggagtctc cttcacaatc agtaataata aactgagatg
     2341 agactccggc tcggaggccg gagagacagt agccgagagg agactccggg tcaagcccgg
     2401 agaagcagaa actgaggagt taatcagttc aatccctgct ttcagcaggc aagtgtgttc
     2461 cttattccgc cttccgtatt ccgcccttcg cctttcttct acccttcctg atgcagctta
     2521 tcaatagcag cagcgagttt gcggtcgcgg tcggtgatgg tatcgccggc gtcatgcgtg
     2581 cacagatgga tctccacctt gttgtacacg ttgcaccagt tgggatggtg gttatgcttc
     2641 tctgctaaaa aagccacacg ggtcatgaaa gaaaaggctt cggtgaagtc ttcgaaaacg
     2701 aaagtgcggc gcagttgatt gttctcttct atccacatgg ttcttcgttt ttattctatg
     2761 atactgatgc ggagcacatt ggttcgcgat ggctgggtga ccggcatggt acccaggttg
     2821 agcacaatgt ctcccttttg taacagtttc tttcctttga gtacttcctg cacgtccatg
     2881 aaggtttcgt aggtgccttt caggcgggtg tagtaaaatg ccttgacacc ccagagcagg
     2941 ttgagtgtat tgacgatctc ctggttgttc gtaaagatgt agatggcagc acgaggtctg
     3001 gaactggaaa ccatgaaagc agtgtaaccc gtcttggtca ttcccatgat ggcacgggcg
     3061 cctacttcat cggccatgtt gcaggaattg tagcagacgg catcgctcag gaaggtgcgg
     3121 ctcttcggat cagctacatg cctgcggttg tagatgatgt cttccttctc tgctttgttg
     3181 atgatgcgtg acatggtctt caccaccagt tcggggtgct gccccatagc ggtctcacca
     3241 ctcagcatga cggcatcggc accatccatg accgcattgg ccacatctgt tacttctgcc
     3301 ctggtcggtc tggcctgttc tatcatactc tccatcatct gggt
//
LOCUS       DAOMAX010000279         3332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5749_length_3332_cov_3.625877, whole genome shotgun sequence.
ACCESSION   DAOMAX010000279 DAOMAX010000000
VERSION     DAOMAX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_3332_cov_3.625877"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PK742_11560"
     CDS             <1..1206
                     /locus_tag="PK742_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77270.1"
                     /translation="GTNLKGNQYYLNADAAAPDNSISGAVEITWDMIPTNGNLFEGAT
                     INGSDEIEIQRKSPFTLIALSPASGTSDTLNKIVNPGTDPRSFLPGDVVVLTPLNGAT
                     MSFIINDATVSSGNMNLGNAQSVYLSGYNCLVLIKDSTGWREMSRTTDVGLESIENDV
                     GTGGTWNILDNSLAAGKFARTKFAAVLIDASGSPTLTTDYTVQGEAIISQRSIAQFAF
                     VTQKGDITLNGKQMTVFGVDIPRELALAGNYVVFAYFDDTIDDYRGFLVVREVSPDWE
                     DFTSLATINATNVSAEDVAVKRKYGSEADDGGELWFGGTITLNAAVTHGTGKPLASSI
                     NSRYRPNSYTQRVQCEVTTPVNPVAISAKYCTIEVTTAGAMNVYAHSGENLASGDIIN
                     LTPLRYISA"
     gene            1206..1877
                     /locus_tag="PK742_11565"
     CDS             1206..1877
                     /locus_tag="PK742_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77271.1"
                     /translation="MVIDVTYLYEYINDLLRKHRNGYLDPETFNRLLKAAELILLDYF
                     FKNLPNDHIRPFVKHVPYLAGDGDGTVAFPDDCAFHDSVGGVMLEEGSLKVYPSTKIQ
                     PNQVNTIKVSRIRPPDYEKRRFYHAFMNNKVYVYPEEYSYWIQFTYYRYPKFGVLGYS
                     AEVVDGEDVQTLTVATPMEWNMITLEYFKSVLCRAVGINLKDQFAIQATALPNFQDVV
                     KPIEQ"
     gene            1874..2497
                     /locus_tag="PK742_11570"
     CDS             1874..2497
                     /locus_tag="PK742_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77272.1"
                     /translation="MIKGKFIELVQEMIHADPTVETVVHDDVIGTFLGPVINYLLYKQ
                     YYIDLKDGETLIGLNGAFAYPFEAEPDYDETRKKWYIDMPYAPIVLTKDRGILYLGPS
                     VAEENEYIPTSQTNQPILSTHYMSLTNRVFYRREGMKRIWFLNMDETNKCKVLGRIVV
                     NADDLGEDDYLPIPSGLEMEAMGMLRDFLLGKRQLPEDNRVNDSDKS"
     gene            2494..3312
                     /locus_tag="PK742_11575"
     CDS             2494..3312
                     /locus_tag="PK742_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77273.1"
                     /translation="MNYHDSYGDKLDAVILKVINLEGLDLSHYANLMIIAETGYKAMF
                     RNHIPEFISEYVNVAEPLSARIINFPRSLQTFLKIGVVVTFNGTSKVLTLARDEHIHL
                     PNDTDIENLTCDCEEASTIAGVINQIEDGLIPLSQYARYYGAFRGGQYVGEMFGYEGG
                     RSSAGGFRIDYNNRRVVISSDAPTDRDYLICFKPNPFYKGPDTIIPEYAVEALMAYIR
                     YNRLNQRNSTPGYRYEMENKWITEQKNMKNFIHLNGVTADDIMGMFYGNGLSPK"
BASE COUNT          931 a          767 c          861 g          773 t
ORIGIN      
        1 gggaccaatc tgaaagggaa tcagtactat ctaaatgcag atgctgccgc ccctgataac
       61 agcatttctg gtgcggtaga gatcacttgg gatatgatcc ctaccaacgg taatttgttc
      121 gaaggggcaa ccattaacgg aagtgatgag attgagatac agcggaaatc tccgtttacg
      181 ctgatcgcac tttctccagc atccggcaca tcggatacac tgaacaagat tgtcaacccc
      241 ggaacagatc cgaggtcatt tctacccggt gacgtcgtgg tgttaactcc tctgaatgga
      301 gcaaccatgt cattcatcat caacgatgca acggtttcca gcggcaacat gaacctgggt
      361 aatgcccaat ccgtgtactt gagtggatac aactgtttgg tgctgatcaa agacagcacg
      421 ggctggaggg aaatgagtcg taccaccgat gtagggttgg agtcaataga aaatgacgtg
      481 ggcactggcg gaacatggaa catccttgac aactccttgg ccgcagggaa gtttgcaagg
      541 acaaaatttg ccgccgtcct tatagatgcc agcggatccc caacactgac gacagactat
      601 accgtacagg gagaggcaat cataagccaa cgcagtatag cgcaatttgc gtttgtcact
      661 caaaaaggtg atataacact taatggcaag cagatgactg ttttcggggt ggatataccc
      721 agagagcttg cgctggccgg caactatgtg gtgtttgcgt actttgacga taccatagac
      781 gactaccggg gattccttgt cgtgagagaa gtaagcccgg actgggaaga tttcacctct
      841 ctggcaacaa taaacgctac gaatgttagc gcagaagatg ttgcggtaaa aaggaagtac
      901 ggaagcgagg cagacgatgg tggtgagctg tggtttggcg gaacaatcac tctgaatgca
      961 gcagtcacgc acggtacagg aaagcctctg gcatcatcta tcaactccag ataccggcct
     1021 aacagctaca ctcaaagagt tcagtgtgag gtaactaccc ctgtgaaccc tgttgcgatt
     1081 tcggccaaat actgtacgat agaagtcaca acagcaggtg ccatgaatgt gtacgctcat
     1141 tcaggagaaa atctggcatc tggtgacatc attaacttga caccactcag atacatcagt
     1201 gcataatggt cattgacgta acatatctgt acgagtacat caatgatcta cttcgcaagc
     1261 acagaaacgg gtatctggat ccggagacct tcaaccggtt gctgaaggcg gctgagctga
     1321 tactgctgga ctatttcttc aagaatttgc ccaatgatca tattcggcca tttgtcaaac
     1381 atgtgccata tctggctggc gatggcgacg gcacagtggc attcccggat gattgcgctt
     1441 tccacgacag tgtaggcggg gttatgttgg aagagggttc gctgaaagta tatccttcca
     1501 cgaagattca gcccaaccag gtaaatacga tcaaggtatc acgcatcagg ccgccggact
     1561 acgagaagcg caggttctat catgccttca tgaacaataa ggtatatgtg tatccggagg
     1621 aatacagtta ctggatccag tttacctatt accggtatcc aaaattcggg gtactcggct
     1681 attcagctga agttgtggat ggagaggatg tgcagacgct gacagtggcc acgcctatgg
     1741 agtggaacat gatcacactg gagtacttca agtctgttct ctgcagggca gtgggcatca
     1801 acctgaagga tcaatttgct attcaggcga cagcgctccc caattttcag gacgtggtaa
     1861 aaccgattga acagtgataa aggggaaatt catagagctg gttcaggaaa tgatacacgc
     1921 tgatcccaca gtggaaacgg tggtgcacga cgatgttatc ggcacattcc tgggtccggt
     1981 gatcaactac ctgctgtata agcagtacta catagatctg aaagacggag agaccttgat
     2041 cggcctcaac ggggcatttg cctacccatt cgaagcggaa ccagattatg acgaaacccg
     2101 taagaaatgg tacatagaca tgccttatgc cccgatagtc ctgacaaaag accggggcat
     2161 cctgtatctc ggcccgtctg tagcggaaga gaacgagtac attcctacct ctcaaacaaa
     2221 tcagcctatt ctgtccacgc attatatgtc gctgacgaat cgggtatttt atcgcaggga
     2281 gggtatgaag cgaatctggt tcctgaacat ggacgagaca aacaaatgca aggtgctggg
     2341 ccgtatcgtg gtcaatgccg atgatcttgg agaagacgac tatcttccta tccccagtgg
     2401 actggaaatg gaggctatgg gaatgctgag agatttcctt ctgggtaagc gacagctgcc
     2461 ggaagacaac agggtaaacg atagcgacaa atcatgaact accacgattc atacggagac
     2521 aagctcgatg ccgtcatttt gaaggtgatc aacctggaag ggttggatct ttcgcactac
     2581 gccaatctga tgatcatagc ggaaactggg tacaaagcga tgtttcgcaa ccatatcccg
     2641 gagttcatca gtgaatacgt caatgtagct gagcctttgt cggcaaggat catcaacttc
     2701 cccaggtcat tgcagacctt cctgaagata ggagtggtgg taacattcaa cggaaccagc
     2761 aaagtcctga cactggccag ggacgagcat atccacctcc caaatgatac ggatattgaa
     2821 aaccttacct gtgattgcga ggaagcctct accattgccg gtgtgatcaa ccagatcgag
     2881 gatggtctta tccctctcag ccagtacgcc cgttattacg gagcattccg gggcggtcag
     2941 tatgttggtg aaatgttcgg gtatgaaggt ggccggtctt ctgcaggcgg gttccggatc
     3001 gattacaaca accgcagggt ggtgatcagt agcgatgctc caactgatcg ggattatctg
     3061 atttgcttta agccaaaccc gttttacaag ggccccgaca ccattatccc tgaatacgct
     3121 gtggaggctc tgatggcgta tattcgctac aatcggctga atcaaagaaa ttccacacct
     3181 ggataccggt atgaaatgga gaataagtgg atcaccgagc aaaagaacat gaagaacttt
     3241 atccacctca acggcgtcac agctgatgat atcatgggta tgttctatgg taatggcctg
     3301 tcaccgaaat gaaacaatgg ctgaaaaaga ta
//
LOCUS       DAOMAX010000280         3294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5842_length_3294_cov_4.638160, whole genome shotgun sequence.
ACCESSION   DAOMAX010000280 DAOMAX010000000
VERSION     DAOMAX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5842_length_3294_cov_4.638160"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..2303
                     /locus_tag="PK742_11580"
     CDS             189..2303
                     /locus_tag="PK742_11580"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HQU77274.1"
                     /translation="MLLVLLGSVFLIVTQVQAQRCGIDAYTQYKISQDPKYAVRLTEA
                     EHAIQEWLDANHIDTRSGGDTYTIPVVVHVIWKTATQNISDAQVMSQIDILNEDFQRM
                     NADTVNTPAGFKPVAGSLPVQFCLAQRDPDDEPTNGIERRETTVTTFGLDDQMKFYAS
                     GGLDQWDPTRYFNIWVCNISGGILGYGEFPTGTVSDTYGVVIDYQYFGDQGTATPPYD
                     LGRTATHELGHCFNLRHIWGDDGSSCGGSDLVDDTPNQGSENYGCPGYPKYDACSPSG
                     DGVMFMNYMDYTDDDCMNMFTEGQADRMWSAIESFYPELLTSDACEPFILLALDAGVT
                     DISQPVGQDCDGSVTPVVTIRNWGANVLNSVTINYSIDGGSPNTYNWSGSLASLATAN
                     VTLPAVTAGDGIHVMEIYTSEPNGSTDMNTMNDSGETTFSVFNTGVSVPVEEGFEAAA
                     FPPAGWSVNNSGAAYGWERTLEASSLGDASVWVNNYDYNDPGAEDDLLSIPVDLAGMS
                     GPLVTFDVAYASFKQGVVERTDALEVLVVTSCDGIATSVYYKEGDDLDTRTPTAFAFT
                     PSSSQWRTDSIDLSSFIGEDYVSVVFRSINGYGNNLYLDNINISDGLANAIGDELRNA
                     ASVYPNPANTELVIEWKGTAATGTIQLTDLAGRTIWAGQVIGSNNEIIRISTAQLPAG
                     IYTLLYVAEGGSVMSEQISVQH"
     gene            2395..2712
                     /locus_tag="PK742_11585"
     CDS             2395..2712
                     /locus_tag="PK742_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HQU77275.1"
                     /translation="MSEQKEKAGQLNIELSEEVAEGVYSNLAIITHSNAEFVFDYIRV
                     MPGVPKAKVKSRIIMTPQHAKRLLRALADNIAKYEASHGEILDQETAIQGIPMTFGPT
                     AKA"
     gene            2690..>3294
                     /locus_tag="PK742_11590"
     CDS             2690..>3294
                     /locus_tag="PK742_11590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU77276.1"
                     /translation="MDRQPRPDVSMGVVPELSIVCPVYQGEHSLTHLVEAVDKNCQES
                     GIIYDLILVDDGSSDNSWLTITELQKRYPITAVKQAANYGQHYAIHAGLRQAKGRKIM
                     VMDCDLQDDPACIPLLLEASGIHRSSVLAFRKTKKNDPYYVVSSWFLNVLLTILTRRY
                     NHYRTGNFGVFDQALIRSILQDGIRNFYFPAAVRIYGGHLPV"
BASE COUNT          894 a          706 c          812 g          882 t
ORIGIN      
        1 acaagcgaga gtaattgata gttatcatgt aaagcaatct ataaaaccgg aaagatcact
       61 ttccggtttt tttgtgcagt tttttaagca gcattcatgg ctatttgatg actaacagca
      121 ttttttgaca gttaactaaa tcgtaacttt gataaataat taaatcactc cttatcatgt
      181 ttaaaaagat gctattggtc ctgcttggat ctgttttcct tattgtaacc caggttcagg
      241 cacagcgttg tggcattgat gcttataccc aatacaagat cagccaggat cccaagtatg
      301 cggttcgcct cacagaggcc gagcatgcca tccaggaatg gctggacgca aaccacattg
      361 atacccgaag tggtggagat acttatacca ttccggtagt agttcacgtc atttggaaga
      421 ccgctactca gaatatatct gatgctcagg tcatgtcgca aattgatatt ctcaatgaag
      481 actttcagcg tatgaatgct gataccgtga atacaccggc cgggttcaaa ccggtggccg
      541 gctctctgcc ggttcagttc tgcctggcac aaagagatcc ggatgatgaa ccaaccaacg
      601 gaattgaaag aagagagacc actgtgacca ctttcggtct ggatgatcag atgaagttct
      661 acgcttcagg aggcctggat caatgggatc ccaccaggta cttcaatata tgggtatgta
      721 atatatctgg tggcatactt ggttatggtg agtttccaac aggtactgtc agcgatacct
      781 acggggttgt cattgactac cagtacttcg gcgaccaggg aacggcaaca cctccatacg
      841 atcttggccg taccgcaaca catgagttag gtcactgctt caacctgcga cacatatggg
      901 gcgacgacgg ttccagctgt ggtggaagcg acctggttga tgacacaccg aatcaggggt
      961 ctgaaaatta cggctgccct ggctacccta agtatgatgc ctgcagtcct tccggagatg
     1021 gagtcatgtt catgaattat atggactaca cagatgatga ctgcatgaac atgttcacag
     1081 aaggtcaggc tgacagaatg tggtcggcta ttgaaagctt ctatcctgaa ctgcttacat
     1141 cagacgcctg cgaacccttt attttgctgg ccctggatgc cggagttaca gatattagcc
     1201 aaccggttgg ccaggactgt gatggaagtg taacccccgt agtgaccatt cgtaactggg
     1261 gtgccaatgt attgaacagt gttactatca attattccat tgatgggggt tcaccgaata
     1321 cttacaactg gtcaggaagt ctggcatcgc ttgctactgc caatgtgacc ctgccggctg
     1381 taactgcagg cgatggaata catgtaatgg aaatttatac atcagaacct aatggatcaa
     1441 ctgatatgaa cactatgaac gatagtggtg aaacaacatt cagtgtattc aatacaggtg
     1501 tatcggttcc tgtggaagaa ggttttgaag cagctgcttt tccacctgcc ggctggtctg
     1561 taaacaacag tggtgccgct tatggatggg agaggacatt ggaagcttcc agtctgggcg
     1621 atgcatccgt atgggtcaac aactatgact acaacgatcc gggtgcagaa gatgatctgc
     1681 tgagtattcc ggttgatctg gcaggaatgt ccgggcctct agtaactttt gatgtggctt
     1741 atgcctcttt caagcaggga gtagtggaaa gaacagacgc actcgaagta ctggttgtta
     1801 cttcctgtga tggcattgcc acatctgttt attataaaga aggggatgat ctggatacca
     1861 gaacaccaac agcatttgcc tttactcctt ccagttcaca gtggcgtaca gacagcatag
     1921 atctgagcag tttcattggt gaggattatg taagtgtggt tttcagaagc atcaacggat
     1981 atggcaacaa cctgtatctg gataacatca atatttctga tgggcttgcc aatgccattg
     2041 gcgacgagct gagaaatgca gcatctgttt atcctaatcc tgcaaatacc gagttggtta
     2101 ttgaatggaa aggtaccgct gcaacaggta ccatacagct aactgatctg gcaggaagaa
     2161 caatctgggc cggtcaggtc attggcagta acaatgagat catcaggatc agtacagcgc
     2221 aactgcccgc aggaatttat acgttgttgt atgtagctga aggaggatct gttatgtctg
     2281 aacagatctc agtgcagcac tgatcaagta aaagataaag cttatcgggc gggccttgcg
     2341 cccgcccgat ccgtttttac aggatggaat atgtactttt gaaaaaagaa ttttatgtct
     2401 gagcaaaaag agaaagccgg ccaattgaat attgaactca gtgaagaagt tgctgaagga
     2461 gtttattcca atctggctat tatcacacat tccaatgcag agtttgtttt tgattacatc
     2521 agggttatgc ccggtgtacc aaaggcaaag gtgaaaagcc gcattatcat gactcctcag
     2581 catgccaagc gtttgttgcg tgccctggct gataacattg caaaatatga agccagtcat
     2641 ggagagattt tggatcagga aaccgctatc cagggtattc ctatgacctt tggaccgaca
     2701 gccaaggcct gatgtttcta tgggtgttgt accggagtta tcaatagttt gtccggttta
     2761 tcagggtgaa cattctctta cacacctggt agaggcagta gataaaaact gccaggaatc
     2821 aggcattatc tatgatctga tcctggttga tgatggaagt tcagacaaca gctggttgac
     2881 tattactgaa ctgcagaaga gatatccgat tactgctgtt aaacaggcgg caaactacgg
     2941 ccagcactat gcaattcacg ctggtctcag gcaggcaaag ggcaggaaga tcatggtcat
     3001 ggattgtgac ctgcaggatg atcccgcctg cattccactg ctgctggaag catcaggaat
     3061 acatcgatct tcggtactgg cttttagaaa gactaaaaag aacgacccgt attacgtggt
     3121 ctcctcctgg ttcttgaacg tcttactgac catattgacc aggcggtaca accattaccg
     3181 cacaggcaat tttggtgtgt ttgaccaggc actgatccga tccattctgc aggatggtat
     3241 ccgcaatttt tactttcccg cggccgtaag aatatatggt ggtcatttgc cggt
//
LOCUS       DAOMAX010000281         3292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5848_length_3292_cov_3.492740, whole genome shotgun sequence.
ACCESSION   DAOMAX010000281 DAOMAX010000000
VERSION     DAOMAX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5848_length_3292_cov_3.492740"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..646
                     /locus_tag="PK742_11595"
     CDS             212..646
                     /locus_tag="PK742_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77277.1"
                     /translation="MSLKSTVITKGEILMLRDSVHFITTVVLDNNNELYRYQSWYGDS
                     LRNGPISLSETELLQLSKTTNEKKWRRKNIDEAVKFIHSEKKKPGKRYFKISSPIFFD
                     EQQQAILIFYLVNEYAERYFIQIYKRDETGKWKLDYESLVWV"
     gene            952..1884
                     /gene="trxB"
                     /locus_tag="PK742_11600"
     CDS             952..1884
                     /gene="trxB"
                     /locus_tag="PK742_11600"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQU77278.1"
                     /translation="MDIIKTLIIGSGPAGYTAAIYAARANLSPVMYTGLEMGGQLMQT
                     TDVENFPGYPKGIMGPEMMEEFRQQAERFGTDIRFGYVAKVDFSGEIKKVWLDGGEEI
                     NAHSVIIATGATAKWLGLDSEKRLNGNGVSACAVCDGFFFKGQDVALVGGGDTAAEEA
                     LYLSKLCTKVHMLVRKDHLRASKIMQERVMNAENIEVHWNTETLEVLGDKTVDAVKVR
                     NNQTNEETTIPITGFFVAIGHKPNTDIFKGILDMDDQGYLKTVPGTTRTNIEGVYAAG
                     DVQDKVYRQAVTAAGTGCMAALEAERWLAEKGIE"
     gene            complement(1885..2160)
                     /locus_tag="PK742_11605"
     CDS             complement(1885..2160)
                     /locus_tag="PK742_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HQU77279.1"
                     /translation="MKIEFTEKAWADFEYWMIHEPDMAMKIKELLRAISENPFQGIGK
                     PEPLKYSLKGFWSRRISREHRLVYQITGKKGEDQKCSVVQCRFHYDD"
     gene            complement(2170..2424)
                     /locus_tag="PK742_11610"
     CDS             complement(2170..2424)
                     /locus_tag="PK742_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HQU77280.1"
                     /translation="MKAVSISHLRTRLKSYFDFVVESMDVIVVPRNNDDDAVVIMSIK
                     EYNSLIETGHLLSTEANRKDLQESIEQLKSGETREFSLDS"
     gene            complement(2519..3196)
                     /locus_tag="PK742_11615"
     CDS             complement(2519..3196)
                     /locus_tag="PK742_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HQU77281.1"
                     /translation="MIMLLPFYLLLASLITQPEPAGTMTDADGNTYKTVRIGDQVWMA
                     ENLRVTHYNNGDPIAFIPDYEAWEKTTEGAYSIYMEDTAAVAAYGLLYNGRVVLDERG
                     IAPEGWRVPTNDDWKELLIYFGIDTTTRLPETTTEKRPPGERVPDMPSVSFLVGPPFD
                     ALSAGGRFYDGRYAGRGNLEYWWSGTPYNSANAWFCGLAYAHKGQRYYEASGDIRQGF
                     GIRLIKE"
BASE COUNT          913 a          784 c          743 g          852 t
ORIGIN      
        1 aagggcgact ggaaagacga cgaccgcctg ctgaagcatt tcgggtatca gtaggcttgt
       61 attacaaaac atgattatga tacggtcaat cttacttttt ctcactgtgt tgacctgcaa
      121 cttgttactc gctcagacaa atagcaaatt aacatacgag ctgattaatg ataagatttc
      181 ctctgattcg tttgctttga acggctttaa gatgtctttg aaaagcactg tcatcacgaa
      241 aggagaaata ttaatgctca gggactctgt tcattttatt acaacagttg tactggataa
      301 caacaatgaa ttatatcggt atcaatcctg gtatggagat agtttgagaa atggaccaat
      361 tagcttaagt gaaacggaac tgcttcaatt gtcgaaaaca accaatgaaa aaaagtggcg
      421 tagaaagaat attgatgagg ctgttaagtt cattcactct gaaaagaaaa aacctggaaa
      481 acggtatttt aaaatctcaa gtcccatttt tttcgacgaa caacagcagg ccattcttat
      541 tttttactta gtaaatgaat atgctgaacg atattttatc cagatataca aaagagacga
      601 aactggcaaa tggaagcttg attatgaatc tttagtttgg gtttagaaga gaaaaaatca
      661 aacgattttt cctgaaataa ttgcaccctg actccagaaa taaagaaaat ccattgtttt
      721 taaaagtaca acccaacagt tcccttcctt ccaaccaaac tgccttctgc cctgcacaat
      781 tcccccatat cagttcaatc ccagcctgac ggcaggcagg tgttaaatcc gtgttccgcc
      841 ttaggtaatc cctgaaatca gtccaatctt ttaaatccgt gttccgcctt cacccgccct
      901 taaaggaacc attgttaaat ttgctcgctt tactacctgg aatctaagag tatggacatc
      961 atcaagacac ttatcatcgg atccggccct gccggttata ctgctgccat ctatgccgcc
     1021 cgtgccaacc tcagcccggt catgtacacc ggactggaaa tgggcggaca gctcatgcag
     1081 accaccgatg tagagaattt tcccggctac ccgaaaggta tcatgggccc ggaaatgatg
     1141 gaagagttcc ggcagcaggc cgagcgtttt ggcaccgata tccgcttcgg ctatgtagct
     1201 aaagtggact tctccggcga gatcaaaaag gtttggctgg atggtggcga agagataaac
     1261 gcccattcgg tcatcattgc cacgggtgct actgccaagt ggctgggact ggattcagaa
     1321 aaacgactga acggcaatgg cgtaagtgcc tgtgcggtct gcgatggttt cttcttcaaa
     1381 ggtcaggatg tggccctggt gggtggcggt gatactgctg ctgaagaagc attatacctc
     1441 tccaagcttt gcaccaaagt gcacatgctc gtgcgcaagg accacctgcg tgcttccaag
     1501 atcatgcagg aaagggtgat gaatgcagag aacattgaag tgcactggaa caccgaaaca
     1561 ctggaggtac ttggcgacaa gaccgtggat gcagttaagg ttcgcaacaa ccagacgaac
     1621 gaagagacca ccattcccat caccggtttt tttgtggcca tcggacataa accgaacaca
     1681 gatattttca agggcatcct ggatatggat gatcagggat acctgaagac cgtacccgga
     1741 accacccgca ccaacatcga aggtgtatat gccgccggcg atgtacagga taaggtctat
     1801 cgccaggcag tgaccgctgc cggtaccggc tgtatggctg cactggaagc agaaagatgg
     1861 ctggctgaga agggtatcga gtgatcagtc atcgtagtga aaacgacact gaactactga
     1921 gcacttttga tcttcacctt tctttccggt gatctgataa acaagcctgt gctctctgga
     1981 aatacgccgc gaccagaatc ctttcagact atactttagt ggttcaggtt tacctatacc
     2041 ctgaaatgga ttttcagata ttgccctcag aagctctttg atcttcatag ccatgtccgg
     2101 ttcatgtatc atccagtatt caaaatctgc ccaggctttc tccgtgaact cgatcttcat
     2161 aacaaagcgt caggaatcaa ggctgaattc tctggtctca ccgcttttca attgttctat
     2221 tgactcctga agatcttttc tattggcttc agtggacaat aagtgaccgg tctcgatcag
     2281 ggaattgtat tccttaattg acataatgac aacagcatca tcatcattat tacggggtac
     2341 gactatcaca tccatacttt ccacaacaaa atcaaagtat gacttcagtc gggttcgtaa
     2401 atgggaaatt gaaaccgctt tcataaacaa aacttgtaca atctaaggta cgtaattttg
     2461 tacaaattgt ttcagttgta ataaaagaac aaccgcgaac cagtgcaggc ttcctgattc
     2521 actctttgat cagccggatg ccaaagccct gcctgatgtc accactggct tcataatagc
     2581 gctgaccttt atgcgcatac gccagtccgc agaaccaggc gttggccgaa ttatacggtg
     2641 tacctgacca ccagtattcc agattgcccc tgcctgcgta cctgccatca taaaacctgc
     2701 ccccggccga aagtgcatca aaaggaggac ccaccagaaa agaaacagaa ggcatatcag
     2761 gaacacgctc gccgggtggc ctcttctctg tagtggtctc cggcagtctg gtggtagtat
     2821 ctataccaaa atagatgagc agttccttcc agtcgtcatt ggtgggtacc cgccaccctt
     2881 ccggagcaat accccgctca tcaagcacca cgcggccatt gtacaacaag ccataggcag
     2941 ccaccgcagc agtgtcttcc atatagatgc tgtatgcacc ttcggtggtc ttctcccagg
     3001 cttcgtaatc cggaatgaat gcgatcggat cgccattgtt gtaatgggtg acacgcaggt
     3061 tctcggccat ccagacctga tcgccgatgc gcacggtctt gtaggtgttt ccatctgcat
     3121 cggtcatggt tccggcgggt tccggctggg tgatcagcga tgcgaggagg aggtagaatg
     3181 ggaggagcat gatcatttta tattctggat gctattatcc cgcaaattac atacctgata
     3241 ttgaactatc agcaacacca ttcgttgatt atatgatgga tcagcattct at
//
LOCUS       DAOMAX010000282         3283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5874_length_3283_cov_4.223048, whole genome shotgun sequence.
ACCESSION   DAOMAX010000282 DAOMAX010000000
VERSION     DAOMAX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5874_length_3283_cov_4.223048"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="PK742_11620"
     CDS             <1..1273
                     /locus_tag="PK742_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQU77282.1"
                     /translation="ALMDDRLVLLAWLVAGMFTMFGVFSYAGLATLTSEAGGQMEYFR
                     LVYGRFWAFLYGWASFAVIQTASIASIAYVFAESVNSVLPLPDWLAACDSIRLFGLTP
                     FANSGVKLLAVATILILTLINYFGVEEGGWLNNVLTLAKVTGIIMLIWMGFHYGQGTA
                     DQAPGVETARHLSRNWNDLMGPFFLALLSAFWAFDGWINVTFITGEIRSPQRNIPIAI
                     IAGTAVVMLLYYLVNMAFFRVMGPEEMAAIHTAGDRVVGVEVALRAMGPGGLWAIAVL
                     IMLCTLGATNASLMSTARIYYRMANTGLFFKAAGQAHPRFQTPHKALLMQMVWSVILV
                     FSGTFDQLTDMLIFASFIFYGSGALGLLIMKRRRVIVSRVIGYPVLPVVFVLFCILLV
                     GNTLISMPHESLTGLGLIALGVPLYAYFNKE"
     gene            complement(1323..2210)
                     /locus_tag="PK742_11625"
     CDS             complement(1323..2210)
                     /locus_tag="PK742_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77283.1"
                     /translation="MKRMLFVLAIAPVLFSCNQKKIDDLESQVSELSGKNAEYEQAMA
                     LRDEQINDYFNTMNEVEANLEEIKKHEGIITTQLSGEGSNQKDNIVSSINALNDLIQK
                     NKKLVADLDKKYKNANFKIKELDKQIAMLNEQIAAQEAELADLKQQLIASNYKIETLT
                     ADLDNVTAANDRLEEENKKQSNTIAKQTEDLNTAYYVVGTYKDLKERNILTKEGGFIG
                     IGRNTELNDDFDASAFTKVDIRSFNELDLNVKGASVVTDHLASSYELTGDKKSIESLK
                     ILDYSQFWKTSKYLVVVTE"
     gene            2412..>3283
                     /gene="ruvB"
                     /locus_tag="PK742_11630"
     CDS             2412..>3283
                     /gene="ruvB"
                     /locus_tag="PK742_11630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQU77284.1"
                     /translation="MKNPNLDDTGEYMSRTEREVERVLRPQAFEEFSGQPQLVENLKI
                     FIEAARQRGDALDHVLLHGPPGLGKTTLSHIIANELEVQIKVTSGPVLEKPGDLAGLL
                     TSLEPRDVLFIDEIHRLSTVIEEYLYQAMEDFKIDIMIETGPNARSVQIKLNPFTLVG
                     ATTRSGMLSAPLRSRFGITNRIEYYPASVLQKIIKRASGILNMPIDEEGSMEIARRSR
                     GTPRIANNLLRRVRDFAQIKGGGVINVEIARIGLEALHVDEHGLDEMDNRILLAIIDK
                     FKGGPVGLSTIATAV"
BASE COUNT          718 a          780 c          839 g          946 t
ORIGIN      
        1 tgcgctgatg gatgaccgtc tggtcctgct ggcatggctg gtggccggta tgttcaccat
       61 gttcggggta ttcagttatg caggacttgc tacgctcaca tcggaggccg gcggtcagat
      121 ggagtacttc cgtctggtat atggccgctt ctgggccttc ttatacggat gggcaagttt
      181 tgcggttatt cagaccgcat ctattgccag tatagcctat gtgtttgctg aatctgtgaa
      241 cagtgtgctg cctttgccag actggcttgc agcctgtgat tccatccggt tattcggcct
      301 tacaccattt gccaacagtg gcgtgaaact actcgctgta gctaccatac tgatcctgac
      361 cctcatcaat tatttcgggg tggaggaagg tggttggctg aacaatgtac tcaccctggc
      421 caaagtgaca ggtatcatca tgctgatctg gatggggttt cactatggac agggtacagc
      481 tgatcaggca ccgggggtgg agactgccag acatctatca agaaactgga atgacctgat
      541 ggggcctttc tttctggctt tgctcagcgc attctgggcc ttcgatggct ggatcaatgt
      601 gacctttata accggcgaga tccgcagtcc gcaacgaaat attcccatcg ccatcattgc
      661 aggaaccgct gtggtcatgc tgctctatta tctggttaat atggctttct tccgtgttat
      721 gggtcctgaa gagatggctg ccatccatac tgccggcgac cgggtggtgg gtgtggaagt
      781 agccctgcgg gccatgggtc ccggcgggct atgggccatt gccgtactca ttatgctgtg
      841 cacactgggg gctacgaatg catcactcat gtcaactgcc cgcatctatt accggatggc
      901 caatacaggt ttgtttttta aggcagccgg tcaggcacat ccgcggtttc agactccgca
      961 taaggccctt ctcatgcaaa tggtctggtc tgtgatcctg gtattttcag gtactttcga
     1021 tcagttaact gacatgctga ttttcgcatc ttttattttt tatggaagcg gtgcattggg
     1081 tttgctgatc atgaagcgac gaagggtcat cgtttcgcgg gtgatcgggt atccggtgtt
     1141 accggttgtt tttgttctgt tctgcatatt gctggttggc aataccctca tcagtatgcc
     1201 ccatgaaagt cttaccggcc tgggtttgat cgccctgggt gtaccgcttt atgcatattt
     1261 caacaaagaa taaaaaaagg agcccgaagg ctccttttct gttggcttgc tggtgagggt
     1321 gattattccg ttactacaac cagatactta ctggtcttcc agaactgact gtagtcaagt
     1381 atcttgaggc tttcaatgct cttcttgtca ccggtcagtt cataggaact tgccaggtga
     1441 tctgtaacaa cactggctcc tttaacatta agatccagtt cgttgaaact gcgtatatcc
     1501 actttggtaa atgcactggc atcaaagtca tcattcagtt ctgtattgcg gccaattcct
     1561 atgaaaccac cttctttggt caggatgttc ctttccttga ggtccttgta agttccaacc
     1621 acatagtaag ctgtattgag gtcttcagtc tgcttggcaa tggtattgct ttgcttcttg
     1681 ttttcttctt ccagacgatc attagctgcg gtaacattat ccagatctgc cgtcagggtt
     1741 tctatcttat agttggaagc aatgagctgt tgtttcaggt cagccagttc tgcttcctga
     1801 gctgcgatct gctcattgag catagcgatc tgcttatcca gctccttgat cttgaagttg
     1861 gcattcttgt atttcttatc cagatcagct accagcttct tgttcttctg gatcaggtca
     1921 ttgagtgcat tgatgctgct cacaatgttg tccttctggt tggaaccttc gccgctcagc
     1981 tgggtggtaa tgataccttc atgcttcttg atctcttcca gattggcctc tacttcgttc
     2041 atggtattga agtagtcgtt gatctgttca tcacgaaggg ccatggcctg ctcgtattct
     2101 gcattcttac ctgatagctc tgacacctga gattccaggt cgtcgatctt tttctggttg
     2161 caactgaata acacaggtgc gatggccagc acaaacaaca ttcttttcat gtccagttct
     2221 tttggtgagg attattgaaa atatcctgcc agatgggcct gattctttaa aacccttgtc
     2281 tgacaggcat ttagcgcaaa ggtggggcgt ttattgtgca tcctgactgg ggaaatcatt
     2341 cctcatgttg tggaaaccca ctctgttgta cactatttac tatagcccgg aaggaggtac
     2401 ctttatagcg catgaagaac ccgaatctgg acgataccgg tgaatatatg agccgcaccg
     2461 aacgggaggt ggaaagagtg ctgcgccccc aggcctttga agagttcagt ggtcagccgc
     2521 aactggtaga gaatctgaag atcttcatag aagctgccag acagcggggc gatgcactgg
     2581 atcatgtact tctgcacggg cctccgggtc ttggaaagac caccctgtct cacatcattg
     2641 ccaatgaact ggaagtgcag atcaaggtca cctccggccc ggtactcgaa aagcccggcg
     2701 acctcgcagg tctgcttacc agtctggaac ccagagacgt tctctttatt gatgagatac
     2761 atcggcttag tacggtgatc gaagagtatc tgtatcaggc tatggaagat ttcaagatcg
     2821 atatcatgat cgagaccggc ccgaatgcca gaagtgtaca gatcaaactg aatcctttta
     2881 ccctggttgg agctaccacc agaagtggca tgctgagcgc accacttcga tccaggtttg
     2941 gtatcaccaa ccggatcgaa tattatcctg cttctgtctt gcagaagatc atcaaacggg
     3001 catctggtat cctgaatatg cctatagatg aagaaggttc catggagatc gcccgcagga
     3061 gccggggtac gccgagaatt gccaacaacc tgttgcgccg ggtgcgtgac tttgcccaga
     3121 tcaaaggtgg cggtgtcatc aatgtagaga tagccagaat aggcctggag gcactgcatg
     3181 tagatgaaca tgggctggat gaaatggaca acaggatcct cctggctatt attgataagt
     3241 tcaagggagg cccggtaggc ctttccacca ttgccacagc ggt
//
LOCUS       DAOMAX010000283         3272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5906_length_3272_cov_4.811315, whole genome shotgun sequence.
ACCESSION   DAOMAX010000283 DAOMAX010000000
VERSION     DAOMAX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_3272_cov_4.811315"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK742_11635"
     CDS             complement(<1..536)
                     /locus_tag="PK742_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77285.1"
                     /translation="MKRILAGIFMLTVISTQAQTEPATKNFKYDFFSPVAGCFGFDLE
                     TWYRNSVTMEYKAGLIGLKLGDYFGNEQFIGGYFSIGPKFYFQLDKMEGIDNYSDFSG
                     FYMKPEILLNYFSFTDTYTQYEWWFDDFGNYYENLTDYEVKGSDMSVSLMMGLGRQWT
                     FRDIVTLDLWFALGYGGN"
     gene            complement(613..927)
                     /locus_tag="PK742_11640"
     CDS             complement(613..927)
                     /locus_tag="PK742_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77286.1"
                     /translation="MRSVLFSLALLAGVIVAPNAVHAQDSKSVSLTLHNGSAVSIPLK
                     IEKVMNPNLSPFSNSAVTVAEGTRIWYRKENKWVDFLVVTADLNGTVVEVDELLKEKG
                     LK"
     gene            1072..1650
                     /locus_tag="PK742_11645"
     CDS             1072..1650
                     /locus_tag="PK742_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQU77287.1"
                     /translation="MSPRTAIQNEQIREKSREKIVLAAMELFSKQSFHRTSVADIART
                     AGISKGLIYNYFETKEDILRGIIDYMFAIGDQIMEESRAEGDPRKELRHMIEQMFAFL
                     QQQAHISRMLIPLALEMGQFTFINEIIERKAEHYLGRLVRILEELGYEDPEMEAWMLG
                     ILFDGIPLDYMMLGERMPIKRIEQYIYKKYNL"
     gene            1647..2387
                     /locus_tag="PK742_11650"
     CDS             1647..2387
                     /locus_tag="PK742_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HQU77288.1"
                     /translation="MRNWIMALAVCMPMLLQAQSATEVVAKADQVMRGKSSIADMTIQ
                     IVRPSWSREMKMKTWSRGTEQSAVLITAPAKDAGTVFLKRDKEVWNWVPAIERTIKMP
                     PSMMSQSWMGTDFTNDDLVKEFSVVNDYTHKFVGEETIAGRSCWIVEMIPKPDAAVVW
                     GKVKVWIDKKDYLQLKVENYDEDGYLVNSMLAYDIKNLGGRMIPSRMEMIPADEPGNK
                     TVMIYNNIQFDVAIDQEVFTVDYMKRLK"
     gene            2384..>3272
                     /locus_tag="PK742_11655"
     CDS             2384..>3272
                     /locus_tag="PK742_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77289.1"
                     /translation="MIYLKLAWRNLWRNRRRTFITVGSLFFAVLLAIVMFALLEGVYG
                     NMIRNMAGFTTGYLQVHGNGYWEEKDLDLAIPVDEALIDKLDHTKGITGVMPRIESFA
                     LASTGEVSSPVMLMGIDPVREDAVNNLQTRTKEGKFFTAKEQSAAIGEGLATKLSLSV
                     GDTLLLLGQGYHAASAYGKYPIAAIIDMGSPELSKSLVYLPLNEMQRYLSMEGMVTSI
                     ALMSAPGMDLQKELKAVQASIDTTSYHVMDWTEMMPELLQTMQGDQAGNRIMIYILYM
                     VIGFGLFSTVLMMLTERQHE"
BASE COUNT          887 a          811 c          831 g          743 t
ORIGIN      
        1 cagttgccgc cataacccag tgcaaaccaa aggtcgagtg tgacgatgtc tctgaaggtc
       61 cactgtcttc ccagccccat catcagagat acagacatgt cagaaccttt tacttcataa
      121 tcggtcaggt tttcgtagta gttgccaaaa tcgtcaaacc accattcata ctgcgtatag
      181 gtgtcggtga aactgaagta attaagcagt atctccggtt tcatatagaa tcctgagaaa
      241 tcactgtagt tgtcaatgcc ttccatttta tccagctgga aatagaattt aggtccgata
      301 gagaaatatc ctccgatgaa ctgctcattg ccgaaataat cgcccagctt caaacctatc
      361 agcccggcct tgtactccat ggtcacgctg ttgcggtacc aggtctccag gtcgaaacca
      421 aagcatcctg ccacaggact gaagaaatcg tatttgaagt tcttggtcgc gggttcggtc
      481 tgcgcttgtg tgctgatgac cgttaacata aaaatgcctg ccagtatcct tttcatagtt
      541 cgtgtttaca caaaattaag caggaatctg acaggcaaaa gctatgcagg ttaccagacg
      601 gtcagacaaa ggtcatttca gtcccttctc cttcagcagc tcatccactt ctacaacggt
      661 gccattgaga tctgcagtga ccacgagaaa gtcaacccac ttattttctt tccgatacca
      721 gatgcgggtc ccctctgcta cggtcacggc actgttgctg aacggcgaca ggttggggtt
      781 catgaccttt tcgatcttca gcggaatgga caccgctgaa ccgttgtgca gggtcagcga
      841 aacggacttg ctgtcttgag catgcactgc attcggggca acgatgacac ctgccagcaa
      901 tgcgagagag aatagtactg atctcatgtt gggtgttttg aaaggaaaca ggatactgcc
      961 gttttggttg tatttctgta agcagcgatc agttaaagtc ttttaatacc cgaaaaatat
     1021 ttggcagagt cggaaacccg caatactttt gactgaccgt tcagtcaata tatgtcaccg
     1081 agaacagcca tacagaacga gcagatcagg gaaaaaagcc gggaaaagat cgtcctggcg
     1141 gcgatggagc tgttcagcaa acagtccttc caccgcacca gcgtggccga tatcgccagg
     1201 acagcaggca tttcgaaagg tctgatctac aattatttcg agaccaagga agacattctt
     1261 agaggcatca tcgattatat gttcgccatc ggcgatcaga tcatggaaga gagccgtgcg
     1321 gaaggagatc cgcgaaaaga gctccgccat atgatcgaac agatgttcgc cttcctgcag
     1381 cagcaggcac atatcagtcg catgcttatt ccgcttgcgc tggagatggg gcagttcact
     1441 ttcatcaacg agatcatcga acggaaagca gagcactacc tggggcgact ggtccgcatc
     1501 ctggaggaac tcggatacga agaccctgaa atggaagcct ggatgctggg tatcctgttc
     1561 gatggcatcc cactggacta tatgatgctg ggtgaacgaa tgcccatcaa aaggatagag
     1621 caatacatat ataaaaaata taatttatga gaaactggat catggccctg gcggtctgca
     1681 tgccaatgct gctgcaggca caatccgcaa ccgaagtagt ggccaaagcc gatcaggtga
     1741 tgcggggcaa atcatccatc gccgacatga ccatacagat cgttcgcccc tcctggagtc
     1801 gtgagatgaa gatgaagacc tggagtcgcg gtaccgaaca atcggctgta cttatcaccg
     1861 caccggcaaa agatgcaggc accgtttttc tgaagcggga taaagaagtg tggaactggg
     1921 taccggctat cgaacgcacc atcaagatgc cgcctagcat gatgtcgcaa agctggatgg
     1981 gtaccgactt taccaacgat gacctggtca aggaattttc tgtggtgaat gactataccc
     2041 acaagttcgt gggcgaagaa accatcgcag gccgcagctg ctggatcgtg gagatgatac
     2101 ccaaaccgga tgcagctgtg gtgtggggaa aggtaaaagt gtggatcgac aagaaagatt
     2161 acctgcaact caaagtcgag aactacgatg aggacggcta cctggtcaac agcatgctgg
     2221 cctatgacat caaaaatctg ggcggacgta tgatcccttc cagaatggag atgataccgg
     2281 ccgatgaacc cggcaacaaa acggtcatga tctataacaa cattcagttc gatgtagcca
     2341 tcgaccagga agtatttact gtggactata tgaagcgact gaaatgatct acctcaaact
     2401 ggcctggcga aatctctggc gcaacagaag acgcaccttc attacggtgg gttcgctctt
     2461 cttcgccgtg ctgctggcca tcgtcatgtt cgccctgctg gaaggggtct atggcaatat
     2521 gatccgcaac atggcaggtt tcaccaccgg ttacctgcag gtgcatggca atggctactg
     2581 ggaagaaaaa gatctggatc tggctattcc ggtggatgaa gcactcatcg acaaactcga
     2641 tcataccaaa ggcatcacag gtgtcatgcc ccgcatcgaa agttttgcgc tggcatccac
     2701 cggcgaggta tcctctcctg tcatgctcat gggcatcgat cccgtcaggg aagatgcggt
     2761 caacaacctg cagaccagaa ccaaagaagg aaaattcttt actgcgaaag agcagtccgc
     2821 agccatcggt gaaggacttg ccacaaagct ttctctgtca gtgggcgata ccttgctttt
     2881 actcggacaa ggctatcacg ccgcatcagc ctatggcaag tatcccatcg cagccatcat
     2941 cgacatgggc agtccggagc tgagcaaaag tctggtctat ctgccactga acgagatgca
     3001 gcgctacctc tccatggagg ggatggtcac cagcattgcg ttgatgtcgg caccgggtat
     3061 ggatcttcag aaagagctca aagcggtaca ggcaagcata gacaccactt cctatcacgt
     3121 gatggactgg accgaaatga tgccggagtt gttgcagacc atgcagggcg accaggccgg
     3181 caaccgcatc atgatctaca tcctgtatat ggtgatcggt ttcggattgt tcagtaccgt
     3241 actgatgatg ctgaccgaaa gacaacacga at
//
LOCUS       DAOMAX010000284         3250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_5994_length_3250_cov_4.456025, whole genome shotgun sequence.
ACCESSION   DAOMAX010000284 DAOMAX010000000
VERSION     DAOMAX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_3250_cov_4.456025"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK742_11660"
     CDS             complement(<1..304)
                     /locus_tag="PK742_11660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQU77290.1"
                     /translation="MKAAVKITSLLLLSLAWSSYSNPAQLPESDREAAYHMKTVVIDA
                     GHGGHDAGCSGKHSREKDVALKIALELGGMIEKNFPDIKVVYTRKTDVFVELHERAA"
     gene            517..3054
                     /locus_tag="PK742_11665"
     CDS             517..3054
                     /locus_tag="PK742_11665"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HQU77291.1"
                     /translation="MDTLSSDSLVMDSIFMDTIVTWYDISPDSLDAIINYKSVDSIVY
                     DIDSGKTYIYLSGEVTYTSFFLKADYIEFDWETKTLTAKQLVDSAGVPGEAAYFQDGD
                     DTFTAEGMHYNFATRNGKIYNLKRQEGEGYVYVDQGKKNAENEYYGLHTVYTTCDLDH
                     PHFYIAANKAKIVPKKIAVTGPANLVVADVPTPLFLPFGIFPINQGRTSGILIPQYGY
                     SFTQGYFLRNGGYYFAINDNFDLSLTGDIYSNASYRLQTSSGYAKRYKYRGNLSLEYS
                     KNNYGLDFDPNFSSNRGFFVRWNHSQDPKARPGSSFAANASFGTSDFLANNSFTESYL
                     NNSYNSSISYNKSFTGSPFTMSAALRHSQNTSTGLVDLTLPDVSLNMSRIYPLKKVLN
                     DPRNQLNQFGISYSMNFRNFLSVPDSTLFTPESLAKMENGFSHNFSASSPFKLLRYFT
                     LTPSFTYNENWYFETVRKNYEPDTIVTEVFDPVSGELTYDTAIVYFQTDTISGFDAAR
                     WFNASASLTTKLYGIMQFKRGKVKAIRHVVTPSVSFSYRPDFSDPEYGYYGSYYPSPG
                     AEAVTYSIFEGALLGGPPNGKSGSIGINLANNFEMKVRDETDTLKGERKVKLIDNLIL
                     NTAYNLAADSLNWSFINGSAYTTLFNKLRVNVSGSFDPYVLDSTGRRINQFEWDVNDR
                     LARFSTGNVALSTSLRSKRRENENLETTAGTEAERDMVWSNPSAYIDYELPWQMTLNY
                     NLRIQNIQLTDGTDSIATTQTFNLSGDVSLTPNWKVQVSTGYDLQLKDFTYTAIDIYR
                     NLHCWEMSFHWVPFGARQSYLFNINVKASVLQDLKLTRKRDWNEYSF"
BASE COUNT          837 a          803 c          766 g          844 t
ORIGIN      
        1 tggctgcccg ttcatgcagt tctacaaata catccgtctt gcgggtatag accaccttga
       61 tatccggaaa gttcttttcg atcatgccac ccaactccag agcgatcttc agtgccacat
      121 ccttttctct ggaatgcttg ccactgcatc cggcgtcatg cccgccatga ccggcatcaa
      181 tgaccacggt cttcatgtga taagcagcct ccctgtctga ctcaggtagc tgtgcaggat
      241 tggaatacga actccatgca agggataaga gcaggaggct tgttatcttt acggcagctt
      301 tcatatggag gcaatatttg gcctgttaat gcatttgaca tattaaaatt attccaactc
      361 cgcttgatca ggcaagtctc tttgctcata ttccttctgt gcacctgcat atccggccat
      421 attctggctg agaacgcaat ttttgtacca tttattgagc atcagcaggt tgtttctgtg
      481 gatacgtcca aaagttttga ggccgataca agcgctttgg atacactttc ctccgatagt
      541 ctggtcatgg attccatatt tatggatacc atcgtgacct ggtatgacat ctcacctgat
      601 tctctcgatg ccatcatcaa ctataaatct gttgatagta tcgtatatga tatcgactcc
      661 ggaaagacct atatctacct gagtggtgaa gtgacctata cctccttttt tctgaaggcc
      721 gattacatcg aattcgactg ggaaacgaaa acactgactg caaaacaact ggtggactct
      781 gccggtgtgc cgggtgaagc tgcttacttt caggatggcg atgatacctt cactgccgaa
      841 ggtatgcact acaattttgc taccagaaac ggaaagatct acaacctgaa gcggcaggaa
      901 ggagagggct atgtgtatgt ggatcaggga aagaagaatg cagagaatga atactatggc
      961 ctgcatactg tttataccac ctgtgatctc gatcatccgc acttttacat cgctgccaat
     1021 aaggctaaga tcgttccgaa gaaaatagcg gtgacgggcc ctgccaatct ggtagtggcc
     1081 gacgtgccca cacccttatt cctgccattc ggcatttttc ccatcaacca gggaaggact
     1141 tccggcattc tgataccaca gtacgggtac agttttacgc aaggttattt tctgcggaat
     1201 ggtggctact actttgccat caatgacaat ttcgatctct ctctcacggg cgatatatat
     1261 tccaatgcat cttacagatt acagacgtca agcggttatg ccaaaagata caagtatcgc
     1321 ggcaacctga gtctggaata ttcgaagaac aactatggtc tggatttcga tccgaatttt
     1381 agcagcaacc ggggcttctt cgtcaggtgg aaccacagcc aggatccgaa ggcaaggccg
     1441 ggttcttctt ttgctgcgaa tgccagtttc ggtacgtcag acttcctcgc caacaactcc
     1501 ttcactgagt cttacctgaa caactcctac aacagtagta tctcctataa caagtcattc
     1561 acaggttcgc ctttcaccat gagtgcagca ctgcgccaca gccagaatac ctccaccgga
     1621 ctggttgacc tcaccctgcc ggatgtgtca ctgaatatga gtcgtatcta tccgcttaag
     1681 aaagtactca acgatccgcg aaaccagctc aaccagtttg gtatcagtta cagcatgaac
     1741 ttccgcaact tcctcagtgt gcctgattcc accttgttta ctcctgagtc gctggctaag
     1801 atggaaaatg gcttctccca taatttttct gccagttcgc cctttaaact gttgcggtat
     1861 ttcacactta caccgtcctt cacgtataat gagaactggt atttcgaaac agtgcgcaag
     1921 aactacgaac ccgataccat cgttaccgaa gtgttcgatc ccgtcagtgg tgaactgacc
     1981 tacgatactg ccatcgtata tttccagacc gacaccatca gcggatttga tgctgcacgc
     2041 tggttcaacg ccagtgcatc acttactacc aagttgtacg gcatcatgca gttcaaacga
     2101 ggcaaggtga aagccatccg tcatgtggtc accccatctg tcagcttcag ttatcggcct
     2161 gacttcagtg atccggaata cggctactat ggcagctact atccatcacc gggtgcagag
     2221 gcagttactt attccatttt tgaaggggct cttttaggtg ggccacctaa tggtaaatca
     2281 ggcagtatag ggatcaatct cgccaacaac tttgagatga aggtgcgtga tgagacggat
     2341 acgctaaagg gtgaacgcaa ggtgaagctg atagacaacc tcattctgaa tacggcctat
     2401 aacctggcag cagattcact caactggagt ttcattaacg gaagcgccta caccactctg
     2461 ttcaataaac tgcgggtgaa tgtgagtggt tctttcgatc cgtatgtgct tgattccacc
     2521 gggcggcgca tcaaccagtt cgaatgggat gtcaacgacc ggctggcccg gttctctacc
     2581 ggaaacgtag cattgagtac ctccctgcga tcgaaacggc gggagaacga gaacctcgag
     2641 acaacagcag gcaccgaggc agaacgcgac atggtgtggt ctaatccttc tgcctacatt
     2701 gattatgagc tgccctggca gatgaccctg aactacaacc ttcgtatcca gaacattcag
     2761 ctgaccgacg gaacagattc cattgccacc acacagacct tcaacctgag cggcgacgtc
     2821 agcctgacac ccaactggaa ggtgcaggtg agtacaggtt acgatctgca gctaaaggat
     2881 ttcacttata ctgcaataga catttaccgc aacctccact gctgggagat gagcttccac
     2941 tgggtgccat ttggtgccag acagagctac ttgttcaata tcaatgtgaa ggcctcggtt
     3001 ctgcaggacc tcaaactgac acggaagcgt gactggaatg agtatagttt ttagtatcgg
     3061 gtatcgggta ttgagtagtg agtattgagt agagggagag tgtgagagtg tgagtgggag
     3121 agtgtgagtg ggagggtgat agatgaacgg aaactgagac aggagattcc tgatatgggc
     3181 gacacaggag cttcacttcg tcggcttcag gaatgacatt tctctttgga aatgtttgaa
     3241 gattatagac
//
LOCUS       DAOMAX010000285         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6049_length_3229_cov_2.985822, whole genome shotgun sequence.
ACCESSION   DAOMAX010000285 DAOMAX010000000
VERSION     DAOMAX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6049_length_3229_cov_2.985822"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /locus_tag="PK742_11670"
     CDS             complement(<1..2151)
                     /locus_tag="PK742_11670"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77292.1"
                     /translation="MGITDVMLYVDGLSAGTTYYIMVDGFGGADGEFGICVEDMSPYP
                     SPSGDDCPEAIFLCEEEDLVVVPDGTGGDGTLEEVPSCFGSGEIASWWYTFTAANTGT
                     LTFDITPSGATNFDWAFYDISGSPGSMCNLSDELACNSAVTTGVTGAGCAGIACEPTL
                     SLIAGHTYALLINRETAAFSSGFTLSFGGTVNFDSPETEFEYDPVVCVGESDGFEVEG
                     GGDGMYYWSFGDGGVSTGDEPNHTYTSAGTYEVVLISFSPGGCYAVATETVIVSDGPD
                     LSIDPEDPVICLGDGVTLDLSYVLTEDCEPLVFIMDEGYDIPEGGVLSIPIPVSGVAP
                     GVMTADLLESVCIDVEHDTDSEVDIWLECPDGTQIELSTDNGGTGNDYNGTCFVLSGA
                     PPVTSGTAPFAGSYLPEQPLSDFAGCTSNGIWYLNITDDTPGGGEGSIEFASLTFACD
                     NTPDIVWSPATGLSSTTVEDPYASPVVTTTYTVTVTDGSGCTADTTVTVTVVDGASAG
                     FIYTGSPYCPDDSDPLPVLDAGAVSGTWSATPAGLVIDAVTGAVDLSVSVPGTYTITN
                     AIGAIGSCPPTSATFEIVIGEDAAILLDDDFCAGDTYILPDGTSTTTGGSFVFNLTAA
                     SGCDSVITLTLVAYSATASTIDASVCSGAAYVLPDGSTTTTAGTYTTTLTNAFGCDSV
                     VTTNLTVDPAVVTDISGSICDGDTYTLPDGSTTTS"
     gene            complement(2121..2486)
                     /locus_tag="PK742_11675"
     CDS             complement(2121..2486)
                     /locus_tag="PK742_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77293.1"
                     /translation="MLQISYSATKVRTIEQAIAVESHALSGYRKEYGDHVARAVVVLA
                     ICDLIDFFNVGQPMNDRQIAQTADLVLDKFWMLKLEDLRVCFDRAKSGKYGQVYNRID
                     GQVIMTWMQVWELQMSCCM"
     gene            complement(2443..>3229)
                     /locus_tag="PK742_11680"
     CDS             complement(2443..>3229)
                     /locus_tag="PK742_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77294.1"
                     /translation="QYKVLWDYMTCDCDNAGIWVPDFEITNIYLGTTPATSVTKEQAL
                     HLYNSDQERIVQLQNGRWWIRTFVEFQYRKLSPANPAHRPIIAALVKYGLATPDGQLL
                     KGHGRPFEGPMDKDKEMEEEMEGGKDGPGPAENPFQETAGLYGFWAVYARFFPDHIHD
                     RDEDTPALGRISRFLARWLKNNGISGTTPLEEWERLAEAIKDHDFWGQKSLKSISNNL
                     QTVIQEVRYDKQTARKDKRQGSTGRTIDRLAADILQRNEGKDD"
BASE COUNT          810 a          843 c          781 g          795 t
ORIGIN      
        1 cgaagtcgtc gtactgccgt cgggtaaagt gtaggtatca ccgtcgcaga tggatccgga
       61 aatgtcggtt acaacagccg gatcaacggt caggttggtg gtgactacag aatcacaacc
      121 gaatgcattg gtcaacgtgg tggtatatgt accggctgtg gtagtagtgg aaccatcggg
      181 caacacataa gctgcgcctg aacagaccga tgcatctata gtggaagcag tggcagagta
      241 tgccaccagg gttaatgtga tcacactatc acatccggaa gcagcggtga ggttaaacac
      301 aaaggagcct cctgttgttg tggacgtgcc atcaggaagt atataggtat cgcctgcaca
      361 aaaatcatca tcaagcagaa tggctgcatc ttctccaatt acgatctcaa atgtggcaga
      421 agtgggggga caagaaccta ttgcaccaat cgcattggtg atggtatagg tgccgggtac
      481 ggacaccgac agatctacag ctccggtaac agcatctatc accagtcctg caggagtagc
      541 gctccatgtt ccggatacgg caccggcgtc cagcaccggc aaagggtctg aatcatcagg
      601 acagtacgga gagccggtat agataaaacc ggcagatgcg ccatccacca ctgtaactgt
      661 aacagtggta tctgcagtac acccgcttcc atctgttact gtaacggtat aggttgtggt
      721 aactacaggg ctggcatagg gatcttccac agtagtagat gaaagacctg ttgccggcga
      781 ccagactatg tcgggtgtat tgtcacatgc gaaggtgagg gatgcaaact caatgcttcc
      841 ttctccgcct cccggtgtat catcagtaat attcagatac cagataccat tggatgtgca
      901 gcctgcaaaa tcagacaggg gttgttcagg taaatagctt cctgcaaaag gagcagttcc
      961 ggaggtgaca ggaggtgcgc cggatagcac aaagcaggtt ccgttatagt cgtttccggt
     1021 tcctccatta tcagttgaca attctatctg ggttccatcc ggacattcca gccagatatc
     1081 tacttcagag tctgtatcat gttcaacatc tatgcaaaca ctttccagca gatcagcggt
     1141 catcacacca ggagcaactc cactgactgg aatgggaatg ctcagaactc ctccttcagg
     1201 aatatcatag ccttcatcca tgatgaatac aaggggttca caatcttcgg tcagtacata
     1261 ggagaggtca agggtcacac catctccgag acatattaca ggatcttcgg gatcgatact
     1321 caggtctggt ccgtcagaga cgatgaccgt ttcagtagcg actgcataac accctccggg
     1381 agaaaatgag atcagcacca cttcgtaagt tccggcactc gtataggtat ggttgggttc
     1441 atcaccggtg gaaacgccac cgtcaccaaa gctccagtaa tacataccat cgcccccgcc
     1501 ttccacttca aagccgtcag attctcctac acatactacg gggtcatatt caaactctgt
     1561 ttcaggggag tcaaaattga ccgttccacc aaaagacagg gtaaagccac tggaaaaagc
     1621 tgcagtttcc cgattgatca acaaggcgta ggtatgacca gcaataagcg agagggttgg
     1681 ttcacaggca attcctgcac aacccgctcc ggtgacacca gtggttacag cgctgttaca
     1741 ggccagctca tctgaaagat tacacatgct accaggtgaa ccactaatgt cataaaaagc
     1801 ccagtcgaaa ttggttgcac cggatggtgt aatatcaaat gtcaaggtac ctgtgttggc
     1861 agcagtaaag gtgtaccacc aggatgcgat ctctccgctt ccaaaacagg aaggcacttc
     1921 ttccagggta ccatcacccc cggtgccgtc tggtaccacc accaggtctt cttcctcaca
     1981 aagaaaaatg gcttccgggc aatcatcacc ggagggagat ggatatggcg acatatcttc
     2041 cacgcagata ccgaactcac cgtcggcgcc tccaaaacca tctaccatga tatagtaggt
     2101 ggttcccgcc gacaggccat ctacatacaa catgacatct gtaattccca aacctgcatc
     2161 caggtcatga tgacctgtcc atcgatccgg ttatacacct ggccgtattt acctgatttt
     2221 gctcggtcaa agcaaacgcg gaggtcttcc agtttcagca tccagaactt gtccagtacc
     2281 aaatcggcgg tctgtgctat ctgtcggtca ttcatcggtt ggccaacgtt gaagaaatct
     2341 atcaggtcac agatggctaa cacaacaacg gccctggcca catggtcacc gtattccttc
     2401 cggtaccctg aaagggcatg gctctccacg gctatagcct gttcaatcgt ccttaccttc
     2461 gttgcgctgt aggatatctg cagcaagtcg gtcgattgta cggcctgtac ttccttgcct
     2521 tttgtctttt ctggcagttt gtttgtcata tcttacctcc tgtattacgg tttgtaaatt
     2581 gttgctgatg gatttcaggc ttttctggcc ccaaaaatca tggtctttta tggcctctgc
     2641 gagccgttcc cactcttcca gtggtgtagt accactgatg ccgttatttt tcaaccaacg
     2701 ggccagaaaa cggctaattc ggcccaatgc gggtgtatct tcatcccggt catggatgtg
     2761 gtcagggaaa aaccgggcat agacggccca aaatccatat agtccggccg tttcctggaa
     2821 gggattttct gcagggccag ggccatcctt tcccccttcc atttcctctt ccatttcctt
     2881 atccttatcc ataggccctt cgaagggcct tccatgcccc tttaatagct gtccatctgg
     2941 tgtggccaag ccatacttca ccagggcggc aatgattggt cggtgagccg ggttggccgg
     3001 gctaagtttc cggtactgaa attccacaaa tgttcgtatc caccaacggc cattttgcag
     3061 ttgcacgata cgctcctggt cactattgta caggtgcagg gcttgttcct ttgtgacgga
     3121 tgtggcaggg gtggtgccaa ggtatatgtt ggttatctca aagtctggca cccatattcc
     3181 agcattgtca cagtcgcaag tcatgtaatc ccatagcacc ttatactgc
//
LOCUS       DAOMAX010000286         3209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6103_length_3209_cov_3.816424, whole genome shotgun sequence.
ACCESSION   DAOMAX010000286 DAOMAX010000000
VERSION     DAOMAX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6103_length_3209_cov_3.816424"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK742_11685"
     CDS             <1..305
                     /locus_tag="PK742_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQU77295.1"
                     /translation="SYLINLCANDVDLLEKSKNAFVDELTRCEQLGIPYLNFHPGSHT
                     GMGEEEGLKIIAESINIAHDKTKNFNVSSMLEITAGQGTALGYRFEHIARIIELVE"
     gene            complement(402..674)
                     /gene="rpmA"
                     /locus_tag="PK742_11690"
     CDS             complement(402..674)
                     /gene="rpmA"
                     /locus_tag="PK742_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQU77296.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGVKLWGGQDVIAGNIIIRQRGTK
                     YHPGKNVGLGRDHTIFALIDGKVKFTRGKDDRSIVHVLPVEAQGEA"
     gene            complement(687..995)
                     /gene="rplU"
                     /locus_tag="PK742_11695"
     CDS             complement(687..995)
                     /gene="rplU"
                     /locus_tag="PK742_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQU77297.1"
                     /translation="MYAIVNIAGQQFKVEENQEIFVHRQEGKEGDTLEFSEVLLKDND
                     GKVEVGVPTVPNASVSATILSHEKGDKVIVFKKKRRKGYQVQNGHRQSFTKLKIEKIK
                     "
     gene            1149..1234
                     /locus_tag="PK742_11700"
     tRNA            1149..1234
                     /locus_tag="PK742_11700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Leu,seq:caa)
     gene            complement(1386..1880)
                     /gene="hscB"
                     /locus_tag="PK742_11705"
     CDS             complement(1386..1880)
                     /gene="hscB"
                     /locus_tag="PK742_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HQU77298.1"
                     /translation="MEYFELFGLPVSFHMDTADLRKRYLKLSRETHPDFHSLSSDLEQ
                     AAALDRSTRITNAYNVLRDEEKRMKYILMQKGLLEEEARVQLRPEFLMEMMDLNEAMA
                     MADGEDEKDSLRKQIEGIRQELLEIVSPALDAFDQNGDESQLSEVVDFYHKQRYLVRL
                     LEQL"
     gene            complement(1880..2695)
                     /locus_tag="PK742_11710"
     CDS             complement(1880..2695)
                     /locus_tag="PK742_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77299.1"
                     /translation="MKQILFLVFTAAILSVSAQTEGELIDARPVRVMIVPYHPNYYLS
                     DADQELAEINEKKPDELSRIFRYGLDFSMQVQVQQQFEAYSFLRDTAEVADAELRSMY
                     SGITYKYQKPFDLLSDDKVEERKDTHDGDIFGIHQTNEEEKEKRKRWGQPDEDEVELP
                     KEYMNAVVKDPTVLADMSGRYGVDYFIFLNQFELKTNYETCLDRASHNFQREVLVHYS
                     IYDRHGNQLKGSVVSVLFGSNDNRLDLIIGEYLPQITAGIQRELLGQVLKDND"
     gene            complement(2765..>3209)
                     /locus_tag="PK742_11715"
     CDS             complement(2765..>3209)
                     /locus_tag="PK742_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQU77300.1"
                     /translation="GFVADVSLPDNYQTTDYKLGSAFVYGARDAFEVFFYQAVGMWKL
                     VTGQIKATESIQSPIGITQYFPKIWDWRSFWRLTALLSMVLAFMNLLPIPALDGGHIL
                     FIAVEGIIGRKLSDKFMERAQVVGMVLLLSLMVFAVGNDLFKIFQ"
BASE COUNT          778 a          799 c          769 g          863 t
ORIGIN      
        1 attcttattt aattaatctt tgtgcaaacg atgtggattt acttgaaaaa tcgaaaaatg
       61 cgtttgttga tgaattaaca agatgcgaac aacttgggat tccttatcta aactttcatc
      121 caggttctca tactggaatg ggagaagaag aagggttaaa aataattgct gagtctatta
      181 atattgcgca tgataaaact aaaaatttta atgttagcag tatgctagaa attactgcag
      241 gacaaggcac tgcacttggt tatagattcg aacatattgc aaggataatt gaacttgttg
      301 aataagaaca atcataacac gaatattgct ttaatagccc tatcacccaa caaaaagccc
      361 cgaccatcag gtcagggctg tatatcttgt tccggtgagc tttatgcttc tccctgtgct
      421 tcaacaggaa gtacgtgcac gatggatctg tcatccttac cacgggtgaa cttcactttg
      481 ccgtctatca gggcaaatat ggtatgatcg cggcccaggc caacattctt accgggatgg
      541 tacttggtac cacgctggcg gatgatgatg ttacctgcga tcacatcctg accaccccag
      601 agtttcacac cgaggcgctt gctgtgcgat tcgcgaccgt tcttggaact acctacacct
      661 ttcttatgag ccatgatact ggaattttat ttgatcttct cgattttcag tttggtgaag
      721 gactggcggt gaccattctg tacctggtag cctttccttc ttttcttttt gaatacgatc
      781 accttgtcgc ctttttcatg tgacaggatg gtggcagata ccgaggcgtt cggtacggta
      841 ggaaccccta cctctacctt tccgtcgttg tctttcagga gcacttcact gaattccagt
      901 gtgtctcctt ccttaccttc ctggcggtgg acgaagatct cctggttttc ttccacctta
      961 aattgctgtc ctgcgatgtt gacgatagcg tacataggtt ccctgtttca aaagaagatg
     1021 caaagttaat aaggagtagc tgaattcaca acaaggtcaa ttgaaattaa tattgaacag
     1081 ctacaaaggt cgattttaag ctatttttgg gggttgggcg gcggatgttt atttttgcct
     1141 cccctcctgc cgaggtggtg aaattggtag acacgccgga ctcaaaatcc ggttggggta
     1201 acactcagtg tgggttcgag tcccaccctc ggtacatgag catcggaaac cctgccattt
     1261 tggtggggtt tttttgtgga gtaaggcgga gggcgaaatg cggaggacgg aaatcggaag
     1321 gcggaatagg ggaaaaggtc aactgccagc gcagcgaact gccaactgtc aaccgcctac
     1381 tgcagtcaaa gctgttccag caaccttact aaataccgtt gcttgtggta gaagtcgaca
     1441 acctcgctga gttgcgattc atcgccgttc tgatcaaagg cgtccagggc aggtgatacg
     1501 atctccagca gttcctgcct gatgccttcg atctgcttcc gcagtgaatc tttttcatct
     1561 tccccgtcag ccatggccat ggcttcattc aggtccatca tctccatcag aaattccggt
     1621 cgcagctgca cacgggcctc ctcttccagt aaccctttct gcataaggat gtacttcatg
     1681 cgtttctctt catcacgcaa cacattgtag gcattggtga tgcgggtgga acggtcaagt
     1741 gcagcggcct gttccaggtc ggaagagagt gaatgaaagt caggatgagt ttcccggctc
     1801 agtttcagat atcgtttgcg cagatctgcc gtgtccatgt gaaaggaaac aggcaggccg
     1861 aacagctcaa aatattccat cagtcattgt ctttcagcac ctggcccagc aactctctct
     1921 ggatgccggc cgtgatctgc ggcaggtatt cgccgatgat cagatccagg cggttatcgt
     1981 tcgagccgaa caacacagac actacacttc ccttgagctg gttgccgtgc cggtcgtaga
     2041 tcgaataatg taccaacacc tcccgctgga aattgtgcga tgcccggtcc agacaggtct
     2101 catagttggt cttgagttcg aactggttga gaaaaatgaa ataatccacg ccgtaacggc
     2161 cactcatatc tgccagcacg gtaggatctt ttaccactgc attcatgtat tcctttggca
     2221 gctccacttc atcttcatcg ggttgccccc agcgtttccg cttttccttt tcttcttcgt
     2281 tggtctggtg gatgccaaag atatcgccgt cgtgggtgtc ttttcgttct tcgaccttgt
     2341 cgtctgacag cagatcaaaa ggcttctggt atttgtaggt gataccggaa tacatgctgc
     2401 gcaattcagc atccgccacc tctgccgtat cccgcagaaa actgtaggcc tcgaactgtt
     2461 gctgcacctg cacctgcatg ctgaagtcca aaccgtagcg gaagatgcgg ctcagttcgt
     2521 ccggtttctt ctcattgatc tcggccagtt cctgatcggc atcactcagg taatagttcg
     2581 gatggtatgg tacaatcatg acccgtacag gccgggcatc gatcagctca ccttctgttt
     2641 gcgccgatac agataagatc gcagcagtaa ataccaaaaa taagatctgt ttcatggcta
     2701 cacatttgca atgcatgccc gaaaatacag ggaagttccg gggcggcacc cggagttctg
     2761 cacactactg gaagattttg aacagatcat tgcccactgc aaacaccatg agcgacagca
     2821 gcagcaccat acccactacc tgggcacgtt ccatgaactt gtctgacagt ttcctgccga
     2881 tgatgccttc cacggcaatg aaaagtatat gtccgccgtc cagtgccgga atgggcaaca
     2941 ggttcatgaa tgccagtacc atgctcagca gggcggtcag tcgccagaag gaacgccagt
     3001 cccagatctt gggaaaatac tgggtgatac caataggaga ttggatgctt tcggtcgcct
     3061 tgatctggcc tgttaccagc ttccacatgc ccactgcctg gtagaagaac acctcaaagg
     3121 catcgcgggc tccgtaaaca aatgcactgc cgagtttgta atcggtggtt tggtaattat
     3181 caggcaggct gacatcggcc acaaagccc
//
LOCUS       DAOMAX010000287         3189 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6164_length_3189_cov_3.522974, whole genome shotgun sequence.
ACCESSION   DAOMAX010000287 DAOMAX010000000
VERSION     DAOMAX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6164_length_3189_cov_3.522974"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2859
                     /locus_tag="PK742_11720"
     CDS             <1..2859
                     /locus_tag="PK742_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77301.1"
                     /translation="LQEFDKVFIRLSKDKQSLTVGDFDMIRPSDYFLNYYKRTQGALY
                     EGGFQTARGDQIITTAGLASSRGIYTRQNLAVTEGNQGPYKLTGNNGELFIIVLAGTE
                     RIWIDGKLLTRGAENDYVIDYNAGEITFTPKQLINKDKRVQIEFEYSDKSYFRSVVTS
                     NTSWTNPNEKTTYRLHVYSEQDSKNQPLDLTLDSAARAAMVAAGDDPAQAFIPGIDTA
                     GFSTDRILYRLTDSLGYDSVFVYSTDPELAMYALSFTELGTNKGNYILSSTLANGRVY
                     KWIAPVAGVPQGTAEPVILLRTPISRRMITIAADHKTGDHGTIGAEAAFTQNDLNTFS
                     DAGNDDNTGLGTHVYYNHKTNLSDGKILEAGLDYEYITRDFQFIERYRPVEFNRDWNL
                     QTTDKQPEHYAAAGISLSDSSGNRFQAGTSLYARETAYTGFRQSVNWTQYFHRWKLLA
                     GSSLVNASDDSTSTTFLRPDLTFSRSFAKMNGLEAGFRYWAEHNRFSSADSLRSGAFY
                     FDEYQAFIRTPDTSASQFYTEAIVRHDGLPEAGVFKRVNEGFTWNMGGNLPSGRNRLN
                     WLLTWRTLDVLDTTLSQQQDESALLGRLQYGYTTRKGWLTSDIFYELGTGQEPKREYT
                     FVEVEPGQGNYTWFDYNNDGLQQLDEFEVAIYADQATYIQVFLPTSEYIQANVSQFNY
                     VLNLQPRAVWSDVQGIRDVIARFSLVSSMQLSRKVLDDGSFLSYAPVGTVADTQLVSS
                     NAYWQSTLYFNRTNTRFGAEYTFLQTEGKVVLINGPETRDLTRQNITIRWKITDPLVL
                     STGISSGLKSLESIAFSGRSYRTPFVSLEPKITYINGAQYRISLSYLYKSTQNESGAE
                     SLDSHTGLLDLRYNVPGNSTVSSRISYTLADFNGVADSPVGFALLEGLQDGQNILWNL
                     QFDKRLSQVLQMTLSYDGRKTGSADIVHTGRVQMRAIF"
     gene            2890..>3189
                     /locus_tag="PK742_11725"
     CDS             2890..>3189
                     /locus_tag="PK742_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HQU77302.1"
                     /translation="MQYPYQIDSAEAYQKAWQKSVEEPEQFWSDIAGEFVWKKRWDKV
                     LDWNFREPKISWFAGGRLNITENCLDRHLEERGDQPAIIWEPNDPAEHHRILTYRQ"
BASE COUNT          913 a          765 c          751 g          760 t
ORIGIN      
        1 ttgcaggaat ttgacaaggt atttatccgc ctttccaaag acaagcaatc tcttactgtg
       61 ggtgattttg acatgatcag accgtctgac tattttctca attactacaa aagaacccag
      121 ggcgcattgt atgaaggtgg atttcaaaca gcccggggtg atcagatcat tactaccgca
      181 ggtctggcat cttcacgcgg catatacacc cgacaaaacc ttgctgttac cgaaggcaac
      241 caggggcctt ataaactgac cggtaacaac ggcgagctgt tcatcattgt actggcaggt
      301 acggaacgca tctggataga cggcaaactg cttacccgtg gcgcagaaaa tgattatgtg
      361 atagattaca acgcgggcga aataaccttt acacccaaac aactgatcaa caaagacaag
      421 cgggtacaga tagaattcga atattcagac aaaagttatt tcagatcagt agtaaccagc
      481 aatacctcct ggaccaatcc gaacgaaaaa accacttaca gactgcatgt ttatagtgag
      541 caggacagca agaaccaacc ccttgaccta actctggatt cagcagcaag agccgctatg
      601 gttgcagctg gcgatgatcc tgcacaagct ttcatcccgg gaatagatac tgcaggcttc
      661 agtacagacc gcattctgta caggcttacc gacagcctcg gatatgattc tgtattcgtt
      721 tactccacag atcctgaact ggccatgtat gcactcagtt ttaccgagct gggaacgaat
      781 aaaggaaact atatcttaag ctctaccctg gccaatggca gagtatataa atggatagct
      841 cctgttgcag gtgtaccgca gggtaccgcc gagccggtca ttctgttgcg gacacccatc
      901 agcagaagaa tgatcactat tgctgcagat cataagacag gcgatcatgg aaccattggt
      961 gccgaagctg cattcacgca gaatgacctc aacacttttt cagacgctgg aaatgatgac
     1021 aatacaggac tgggaacaca tgtgtattac aaccataaga ccaacctgtc tgatggcaaa
     1081 attctggagg cgggcttaga ctatgaatat atcaccagag actttcagtt cattgaacga
     1141 taccgtccgg ttgaattcaa ccgcgactgg aacctgcaga ccacagataa acaaccggag
     1201 cattacgctg ctgcaggtat aagtcttagt gattccagcg gcaacaggtt tcaggcaggc
     1261 acatcgctgt atgcaagaga aactgcctat accggcttca gacaatcagt caactggacc
     1321 cagtattttc atcgatggaa actactggca ggcagtagcc tcgtcaatgc ttcagatgac
     1381 agcaccagca ccactttttt gcgtcctgat ctgacctttt cacgaagctt tgcaaaaatg
     1441 aacgggctgg aggccggttt cagatactgg gcagaacaca atcggttttc ttcagctgat
     1501 tcccttcgct caggtgcgtt ctattttgat gaataccagg ctttcattcg cacccctgat
     1561 acttcggcgt cacagttcta tacggaagcg atcgttcgtc atgatggtct gcctgaagca
     1621 ggtgttttca aaagggtcaa tgagggtttc acctggaata tgggaggcaa tctgccttcc
     1681 ggcaggaaca ggctgaactg gttgcttacc tggcgaacac ttgatgtgct ggacaccaca
     1741 cttagtcagc agcaggacga atctgctttg ctgggaaggc tgcaatacgg atataccaca
     1801 cgtaaaggct ggctcacctc agatatattc tatgaactgg gaacaggtca ggaaccaaaa
     1861 agagaataca ctttcgtaga agttgaaccc ggacagggaa actacacctg gttcgactac
     1921 aacaacgacg gattgcaaca actggatgaa tttgaggtgg ctatctatgc agatcaggca
     1981 acatacatac aagtatttct tcctacatca gagtatattc aggcaaacgt ttctcaattc
     2041 aattatgtac ttaacctgca acccagggcg gtgtggtctg atgtgcaggg aattcgagat
     2101 gtcatagccc gattttctct ggtctcctca atgcagctca gcagaaaagt actggatgac
     2161 ggcagcttct tatcctatgc tcctgtgggc acggtagcag acacccagct tgtaagttcc
     2221 aacgcctact ggcagagtac cctgtatttc aacagaacca atacccgttt cggggccgaa
     2281 tacacgttcc ttcaaacgga agggaaagtg gtattgatca acggacctga aacacgagac
     2341 cttaccagac agaacatcac catcagatgg aaaataacag acccgcttgt tttaagtacg
     2401 ggcatttctt ccggattaaa atcgctcgaa tcaatagcgt tttctggtcg cagttaccgc
     2461 acaccattcg tatcgctgga gccaaagatc acttacatta acggtgcgca ataccgcatc
     2521 agcctgtcat atctgtataa gagcacccaa aacgaaagtg gtgcagaaag tctggatagt
     2581 cataccggtt tgctggacct gcggtataat gttcccggca acagcacagt aagcagcagg
     2641 atcagctata cccttgctga cttcaatggt gtggcagaca gtcctgttgg cttcgccctg
     2701 ctggaagggc tgcaagacgg acaaaatatt ctctggaatc tgcagttcga taaacgcttg
     2761 tctcaggtac tgcaaatgac cttgtcttat gatggcagaa agaccggctc agctgatatt
     2821 gtgcataccg gtcgtgtgca aatgcgtgcc atcttctgaa agcagtttct tctgccctat
     2881 cttagcttca tgcaatatcc ctatcagata gactctgcag aggcatatca aaaagcctgg
     2941 caaaaaagtg tagaagagcc ggaacaattc tggtctgaca tagcaggtga atttgtctgg
     3001 aagaagcgat gggataaagt gctggactgg aacttccggg aaccaaagat cagttggttt
     3061 gcaggtggaa gactgaatat cacagagaat tgtctggaca gacatctgga agaacggggc
     3121 gatcaacccg caatcatctg ggagccgaat gatcctgcag aacatcatcg gatcctgacc
     3181 taccggcag
//
LOCUS       DAOMAX010000288         3188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6166_length_3188_cov_4.362592, whole genome shotgun sequence.
ACCESSION   DAOMAX010000288 DAOMAX010000000
VERSION     DAOMAX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6166_length_3188_cov_4.362592"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PK742_11730"
     CDS             complement(<1..244)
                     /locus_tag="PK742_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQU77303.1"
                     /translation="MRIFRFQTDLRAYLSDQRNQGLSIGFVPTMGALHEGHLSLLKTA
                     LTQTEIGVVSIFVNPTQFNDTADLEKYPRTPGADIGL"
     gene            392..1198
                     /locus_tag="PK742_11735"
     CDS             392..1198
                     /locus_tag="PK742_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQU77304.1"
                     /translation="MENKRVLVITQEMTPYLNGTEMSEVANELPQYFQSQGDELRVLM
                     PRYGTINERRHRLHEVVRLSGMNIIIDNDDYPLIIKVASLPGSRLQVYFLDNDDLFKR
                     KFIFHDENEQFFEDNAERMVFFCKAVLETVKKFQWAPDIIHCHGWMTSLIPLYLKTAY
                     KNEPVFDNAKVVFSMYHNAIDEKLANTFMEKAPITDSISAKDLEAFKGLDIPSMNKGG
                     ASFADAIVMGSKELDKNLIKFAKGLKKPVLEYIGKDEGYQDKYAEFYNSL"
     gene            1233..2600
                     /locus_tag="PK742_11740"
     CDS             1233..2600
                     /locus_tag="PK742_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HQU77305.1"
                     /translation="MHRMTRSGWILLLLMMLAAAACRKPTLIGDDLIPPDDYLYSERQ
                     DTFSVFTTVLRDDSAVTSNNLFFPLGSLDEEEVGRSTASLYMQVNLPTNNLFLGNNPV
                     LDSLVLVLDYAGLYGDSMAQHSFNVYKVIEPLYASKLYYSDSKVLTLPAAIGRKANFV
                     PNLKDSVTVLGNTMPPQLRIRLTDQLGTEFTEGDTLKFLNDTTFTNFLNGLVVQPDTS
                     GGHSSSMIIVNPYDANSGLTLYYHTDDADSLTAKFPFSGPKFGSYTHDYSGTPVWNYL
                     TTDAPAAGDSVLFMQGLQGLKTKVSIPYIDSLEGIAINKAEIVFPLRSGEMDSLYPFP
                     GRLSFIEGDTLDHNSFFYIDYNSETFISVVDQDFLFSGINYDYGGTLDTVINKFGVSI
                     PAYRFNITRHLQKILQGEIDNEGFMLIVYPGYRIPNHVTLYGTGAADENLKPYLSITY
                     TTINQ"
     gene            2613..>3188
                     /locus_tag="PK742_11745"
     CDS             2613..>3188
                     /locus_tag="PK742_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HQU77306.1"
                     /translation="MCGIVAYLGHRDAYPVIIKGLHRLEYRGYDSAGIALLNGDMRIF
                     KCAGKVSELEALAEGKNTGGTVGIGHTRWATHGEPNTRNAHPHVSNRQKLAIIHNGII
                     ENYASLKTELLKRGHVFESDTDTEVLIHLIEDIEEKESVDLAEAVRIALTQVVGAYAI
                     VIASEENPDLLIAARKSSPLVLGIGDNEYFVA"
BASE COUNT          826 a          822 c          805 g          735 t
ORIGIN      
        1 gaaggccgat atcagcaccc ggagtgcggg ggtatttttc cagatcggcg gtatcgttga
       61 actgcgtggg attgacgaaa atgctcacca ccccgatctc tgtttgcgtg agagccgttt
      121 tgagcagaga gaggtgacct tcgtgcaatg cccccatggt gggtacaaag ccgatgctca
      181 gaccctgatt tcgctggtca gacaggtagg cccggaggtc ggtctggaat cggaaaatgc
      241 gcatggacag tgggttattc gggcttgtat ggcaaagatt tcggccttat tttgtaattt
      301 tgcatcccgt tatgccggga acgataagca aacggcagac cggacaaatg taggcatctg
      361 ccggaatttt agtcaaaaca ataagtaaag tatggagaat aagagagtac tggtgatcac
      421 acaggagatg accccttacc tcaatggtac ggagatgagt gaagtggcca atgaactgcc
      481 gcagtatttc cagagtcagg gtgatgaact tcgtgtgctg atgccgcgtt acggtactat
      541 caacgagcgc cgtcaccgcc tgcacgaagt agttcgtctg agtggtatga atatcatcat
      601 cgacaatgac gactacccgc tgatcatcaa agtagcttct ctgccaggtt ccagactgca
      661 ggtatatttt ctggacaacg acgatctgtt caagcgcaaa ttcatcttcc acgatgaaaa
      721 tgaacagttc tttgaagata atgccgaacg catggtgttc ttctgtaagg ccgtactgga
      781 gaccgtgaaa aagtttcaat gggcaccgga tatcatccac tgccacggct ggatgacctc
      841 tctcatcccg ctgtacctga agaccgctta taagaacgag ccggtatttg acaacgccaa
      901 ggtggtgttc tccatgtacc acaatgccat cgacgagaaa ctggccaata cctttatgga
      961 aaaggctccg atcacggatt ccatttctgc aaaagatctg gaagccttca aaggactgga
     1021 catccccagc atgaacaaag gcggtgcttc ttttgcagat gccatcgtga tgggcagcaa
     1081 ggagctggac aagaacctga tcaaatttgc gaaaggtctt aagaaaccgg tactggagta
     1141 tattggcaag gacgaagggt accaggacaa atacgctgag ttctacaatt cactctgagt
     1201 acaataattc acttcaagga acgatagagg gtatgcaccg aatgacccgc agcggatgga
     1261 tcctgctgtt gctgatgatg ctggctgctg cggcctgccg gaaaccgacc ctgataggcg
     1321 atgacctgat accgccggac gattatctct actccgaacg tcaggatacc tttagtgtat
     1381 tcactacggt actccgcgat gattctgctg taaccagtaa caacctcttc ttcccgctgg
     1441 gaagtctgga cgaagaagaa gtaggtcgtt ctaccgcaag tctgtatatg caggtgaacc
     1501 tgcctaccaa caaccttttt ctgggtaaca atcctgtgct ggattcactg gttctggttc
     1561 tggactatgc cggcctgtat ggcgacagta tggcacagca cagcttcaat gtctataagg
     1621 tcattgaacc gttgtatgca tccaaactgt attattcaga ttctaaagta ttgacccttc
     1681 cggcagccat cggcaggaag gcgaatttcg tccccaacct caaagacagt gtgaccgtgc
     1741 tgggcaacac catgccaccg cagctgcgca tacgtctgac ggatcagctc ggcacagaat
     1801 tcaccgaagg tgataccctg aaattcctga acgataccac cttcaccaat ttcctgaacg
     1861 gtcttgttgt acagcccgat accagtggcg gacacagcag ttccatgatc atcgtgaatc
     1921 cgtatgatgc caacagcgga ctgaccctct actaccatac cgatgatgcc gattcgctga
     1981 cagcgaaatt cccgttcagc ggaccgaaat ttggcagcta tacccacgat tacagtggca
     2041 ctcctgtctg gaattatctg accaccgatg ctcctgcagc cggtgatagt gtgctgttca
     2101 tgcagggatt acagggactg aagaccaagg tcagcatacc gtacatcgac agcctggaag
     2161 gtattgccat caacaaggca gagatcgtgt tcccgctgcg cagcggagaa atggacagcc
     2221 tgtatccctt ccccggccga ctgtcattca ttgagggaga tacgctggat cacaacagct
     2281 ttttctacat cgactataat tcagagacct ttatttctgt cgtagaccag gatttcctgt
     2341 tcagcggcat caattacgac tacggcggta cactcgacac ggtcatcaac aagtttggtg
     2401 tgtccatccc tgcttaccgc ttcaacatca cacgccatct ccagaaaata ctgcagggag
     2461 aaatagacaa tgaagggttt atgctcattg tgtatccggg ctaccggata ccgaaccacg
     2521 taactttgta tggtacgggt gcggcagatg agaacttaaa gccttatttg tcgatcacgt
     2581 acactactat taaccagtaa aaattcctgc ccatgtgcgg aatcgttgcg tatctcggac
     2641 accgggacgc ctacccggtc attatcaaag gcctgcatcg cctcgaatac cgcggctatg
     2701 actcggccgg tatcgctttg ctgaacggcg acatgcggat cttcaaatgc gccggcaagg
     2761 tgtctgagct cgaggccctg gccgaaggca agaacaccgg cggcacggtg ggtatcggtc
     2821 atacccgctg ggccacccac ggcgaaccca atacccgcaa cgcccacccg catgtgtcta
     2881 accggcagaa gctggccatc atccacaacg gcatcatcga gaactacgct tcgctgaaga
     2941 cagagttgct caaacgcgga cacgtattcg aaagcgatac ggacacagag gtcctcatcc
     3001 acctgataga agacattgaa gagaaagaga gtgtagatct ggccgaagct gtgcgtatcg
     3061 cccttaccca ggtagtagga gcttatgcca ttgtcattgc cagtgaagag aaccccgacc
     3121 tgctcatcgc cgccaggaag agctcaccgc ttgtactggg cattggtgat aatgaatatt
     3181 tcgtagcc
//
LOCUS       DAOMAX010000289         3185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6177_length_3185_cov_3.838019, whole genome shotgun sequence.
ACCESSION   DAOMAX010000289 DAOMAX010000000
VERSION     DAOMAX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6177_length_3185_cov_3.838019"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PK742_11750"
     CDS             complement(<1..878)
                     /locus_tag="PK742_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HQU77307.1"
                     /translation="MLEKFGRTLTRLFLRFMPDAFVFAILLTLLCAAGALICMGSTPL
                     EVITSWYNGFFNLLAFAMQIALIIVTGFSIALSPQVGRLIDAIARRISTPSQVYFLVV
                     AIGMLLSMVSFGWIVITAVFARELAERVKGVHYGFLIACVYFSMNSWVLGLSSTIPLL
                     LNTENNYLIQAGTLDAVIPTSVTLGAGLNIAMMVLMIVAAPVLFFLLRPKQANGMELR
                     DLQTGSSVAREETIEEEARQMRLPYRALSDLLNNSRLLTMIMVGMGVAYIIHHLLTRG
                     VDLDLNIMIFLFLVVG"
     gene            962..1462
                     /locus_tag="PK742_11755"
     CDS             962..1462
                     /locus_tag="PK742_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77308.1"
                     /translation="MRLKTRTILLVTGMTNLFWGCPIGLDHPLGEPDKEAILPDLIGV
                     WENTNAEAVVKVVSILQKDAFSYDVLVRERGLDYVLETDTLTGWVTDLNGMTFFYLRP
                     SNEQQYYHYAILERSADQLVVCDMALLEGGTDAVTDRESLRKEIASSIGKPEFGSEKE
                     TWVRID"
     gene            complement(1495..1962)
                     /locus_tag="PK742_11760"
     CDS             complement(1495..1962)
                     /locus_tag="PK742_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77309.1"
                     /translation="MNTLTPLYKLSELKAKITLILLVSTTLIISCTKPDNPLAWESYL
                     GTYTGTAVHAFYHFDADYNPVVDFEDWTYTMTVETGAQDSMLCFLITYNDTLLETYSD
                     VLIDETGLGQQYWGAGSSASGISVQLHEDTIDFYHYVLMGMGSSTSETFQAVK"
     gene            complement(1959..2393)
                     /locus_tag="PK742_11765"
     CDS             complement(1959..2393)
                     /locus_tag="PK742_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77310.1"
                     /translation="MIHRRLYLMLLSGLFFSACSEVDPSLGIERYYGDYSGLALRTYK
                     HLDATNSFFITEYSDWDYFMTVSEDTTENCLNLIVVYNDTTIASLGSVLIDDTGFGEK
                     RWGAGSSTSKLNLQLHDDTIDFYYFQLMGHTNWITIEFQATK"
     gene            2499..>3185
                     /locus_tag="PK742_11770"
     CDS             2499..>3185
                     /locus_tag="PK742_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQU77311.1"
                     /translation="MEINFPEIESKWKSKWAESGLYKVSEDPARPKYYVLDMFPYPSG
                     AGLHVGHPLGYVASDIVSRYKRLKGFNVLHPMGFDAFGLPAEQYAIETGTHPRITTEK
                     NINYYREQLQKIGFSYDWDREVQTCDPSFYKWTQWIFMQLFNSWYDRSARKARPISEL
                     TELLGTSGSSALVATGQCAENISAADWHAMDKKSRQQFLMQFRLAYLDYAEVWWCEAL
                     GTVLANDEVKD"
BASE COUNT          847 a          754 c          828 g          756 t
ORIGIN      
        1 atccccacca ccaggaacag gaagatcatg atgttcaggt ccaggtccac acctctggtg
       61 agcaggtggt ggatgatata agctaccccc atgcccacca ttatcatcgt cagcaatctg
      121 ctgttattga gcaggtcaga gagtgcccgg tagggaagtc gcatctgtct ggcttcttct
      181 tcgatggtct cttccctggc aacagaactt cctgtttgca gatcacgcaa ttccatgccg
      241 ttggcctgtt tcggtcgcag cagaaaaaac aacaccggtg ctgccacgat catcaggacc
      301 atcatagcga tattgagtcc tgcacccagc gtgaccgagg tggggatcac agcatccagg
      361 gtgccggcct gaatgagata attgttttcg gtgttcagca gcagtggtat ggtggatgat
      421 aatcccaata cccaggagtt catagagaaa tacacacagg ctatcagaaa gccatagtgc
      481 acacccttga ccctctctgc cagctcccgt gcaaatactg cggtgatgac gatccagcca
      541 aagctgacca tagacaagag catgccgatg gctaccacca gaaaatatac ctgtgaaggt
      601 gtgctgatac gtctggcaat ggcgtctatg agtcggccca cctgaggaga taaggcaatg
      661 ctgaatccag tcacgatgat cagggcgatc tgcatggcaa aggccagcag gttgaaaaac
      721 ccgttatacc aggaagtgat gacctccagc ggagtggacc ccatacagat cagcgcaccc
      781 gctgcgcaca gcagggtcag caggatggca aacacaaagg cgtccggcat gaagcggagg
      841 aacagtctgg ttaaggttcg gccaaatttt tccagcatga ctctcaggtt gatattgaag
      901 ataaaaattt tgtggcttaa ccatatacat gggaatatcc atccattatc tttgatagaa
      961 tatgagactg aaaacacgca ccatcctgct ggtaaccggc atgaccaacc tgttctgggg
     1021 ttgtcccatt ggcctggatc atccgctcgg tgaaccggat aaggaagcca ttctgccaga
     1081 tctgatcggg gtatgggaga atacgaatgc agaagctgtt gtgaaagttg tttcgatcct
     1141 gcagaaagat gcattcagct atgatgtgct cgtccgggag cggggactgg attatgtgct
     1201 ggaaacagat acactcaccg gctgggtgac agacttgaac ggcatgacct tcttttatct
     1261 gcgtccttcc aatgaacagc agtactatca ctacgctatt ctggaaagat cagcagacca
     1321 gctggtagta tgcgacatgg cattgctgga agggggcacg gatgctgtaa ccgacagaga
     1381 atctctgcgt aaggaaatag cttcatctat aggcaaaccg gagtttggca gcgaaaaaga
     1441 aacatgggta aggattgatt gagtggccgt atgacagaat tattggccca caattcattt
     1501 caccgcctga aaggtttctg aagtagaaga gcccatgccc atcagcacat agtggtaaaa
     1561 atctatggtg tcttcgtgaa gctgaactga gatgccggat gcagaactgc ctgctcccca
     1621 atattgctgt cccaaccctg tttcgtcgat cagcacatca gagtaggtct ccaatagggt
     1681 atcgttgtag gtgatcagga agcagagcat gctgtcctgc gcaccggtct ccacggtcat
     1741 ggtataggtc cagtcttcaa agtccaccac cgggttgtaa tcggcgtcaa agtgatagaa
     1801 ggcgtgcacc gctgtgccgg tataagtgcc caggtatgat tcccaggcca ggggattatc
     1861 gggtttggta caggagatga tcagggtcgt tgagaccagg aggatcaggg tgatctttgc
     1921 cttcagttcc gacagcttgt atagaggggt aagtgtattc attttgtggc ctgaaattct
     1981 atagtaatcc aatttgtatg tcccatcaat tgaaaatagt agaaatcaat tgtgtcatca
     2041 tgtagttgta ggttcagttt ggaggtcgaa cttcccgcac cccagcgttt ctctccaaac
     2101 cccgtatcat ctattagcac agagccaagt gatgcaattg ttgtgtcgtt atagactact
     2161 ataagattca ggcaattttc agtagtgtct tcagaaactg tcataaaata atcccagtca
     2221 gaatactcag taataaagaa tgagtttgtg gcgtccaaat gtttataggt gcgcaaagcc
     2281 agcccagaat agtcaccata gtatcgttca atacccagtg aaggatccac ctcagaacaa
     2341 gctgagaaaa acaacccgga gaggagcatc agatacagcc ttctgtgaat catgtaccac
     2401 aatattactc gcataaggcg gaagaattgt ctgcagccgg aaatatggca attgtctggc
     2461 gttctgatag ttcgcagcct tccgtatttt tgcccgtaat ggagatcaat tttcccgaaa
     2521 tagagtcgaa gtggaagtcg aaatgggcgg aatccggcct gtacaaggtg tcggaagacc
     2581 ccgcccgtcc caaatattat gtgctggaca tgtttcccta tccctccggg gcaggtctcc
     2641 atgtgggtca ccccctgggc tatgtggcca gcgatatcgt atcgaggtac aaacgcctga
     2701 aaggattcaa tgtgctgcac cccatgggct tcgatgcctt tggtctgccg gctgagcaat
     2761 acgccattga aaccggtacc cacccacgca tcacgaccga gaagaacatc aactactatc
     2821 gcgaacagct gcagaagatc gggttcagtt acgactggga tcgggaagtg cagacctgcg
     2881 atccgtcctt ctacaaatgg acgcagtgga tcttcatgca gctctttaac tcctggtacg
     2941 acagaagtgc gaggaaagca agaccgatat ctgagttgac agaactgctc ggtacttccg
     3001 gttcgtctgc actggtagca actggtcagt gtgcagaaaa tatctctgct gccgattggc
     3061 atgcgatgga taagaagtcc aggcagcaat ttctgatgca gttccgtctg gcctatctgg
     3121 actatgcaga agtgtggtgg tgtgaagcac tgggtactgt actggccaac gacgaagtga
     3181 aggac
//
LOCUS       DAOMAX010000290         3179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6202_length_3179_cov_3.800576, whole genome shotgun sequence.
ACCESSION   DAOMAX010000290 DAOMAX010000000
VERSION     DAOMAX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6202_length_3179_cov_3.800576"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PK742_11775"
     CDS             complement(<1..627)
                     /locus_tag="PK742_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQU77312.1"
                     /translation="MEQKPVRRYNAHKVVQGTAQPVEDPLTVEVMLSIYVNGEPYTIT
                     MHTPGDEEALARGLLLSEQVYTEKENPTFKITEANDAGYITAVNISGDKNAFRTEGKV
                     NRTLVSVSSCGMCGKTEAELLLTGVDIPVHESPSAERVQQMFRTMREHQNTFDSSGGS
                     HASAIFDEAGNLLVIKEDIGRHNAVDKAIGQLMLNGTLEQAFCIIVSGR"
     gene            complement(675..1481)
                     /locus_tag="PK742_11780"
     CDS             complement(675..1481)
                     /locus_tag="PK742_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77313.1"
                     /translation="MAINNTHIILFQVAMFSMVSSCIKPFENVEDYLPVVSMDSVVIN
                     DDGNISLYGHILDEGFTDIQHAGFCYSENEEFSILENQLEVTTYGSFFKLELESAIFE
                     NNTVYYFNAWASNKQGYAKGEPISTADITNEVIAPCSFDTNYFYLDAFASSYNVYYAS
                     ALNDAGGDWEITASVSGTLMSIHFRFSEEPSSGIYSSSGSTMFPSDPEKVYVSVTQGG
                     PLFGYLQLNEKIYLNNYGDGSYSVTLCDAVLITTGFGETYHIDTYFLGPY"
     gene            complement(1481..2119)
                     /locus_tag="PK742_11785"
     CDS             complement(1481..2119)
                     /locus_tag="PK742_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77314.1"
                     /translation="MNSRARQLICILLIMMGSHAHAQFAGLQVGRIQPLSNISYFYGN
                     SWQFTLFNQSSFTESDYLRIGQSFEFSPLSVRSDTFNIVYFDDLNYDEVVPGILVIDR
                     AMLFGLSVFVDGCPYQPPGIDLFGFGGIGARFGWFSMSYTRDYYGGTAAPSLSYTGPE
                     YLGGISGRLGVEYEFNEFMLAGLLFERSLNYNFDTNEFVDYYGLGIRIGYYY"
     gene            complement(2153..>3179)
                     /locus_tag="PK742_11790"
     CDS             complement(2153..>3179)
                     /locus_tag="PK742_11790"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQU77315.1"
                     /translation="KQRLGNIYIGQTYDRKPVFARDLKAESAMAILLKDAIKPNLVQT
                     LEGNPAIIHGGPFANIAQGTNTILATKMGLSLGDYVVTEAGFGADLGAEKFMNIKCGY
                     AGFGPDAIVIVATIRALRHHGGAKKEEYNTASLERVQKGFENLQKHIENCGKYGLTPV
                     VAINAFPADSSEEVQFVIDACARLGAKAVVSEGFAKGGEGTMNLAQAVVDMIESGANK
                     FHRLYDWNLPVEDKIRTIAKEIYGADDVNFSSKALLQLKTIKNLGFDNLPVCMAKTQK
                     SLSDNEKIVGRPTGFSVTVREFEFASGAGFVIPILGDMMRMPGLPEHPASEGMDIDDN
                     GKITGLS"
BASE COUNT          787 a          789 c          760 g          843 t
ORIGIN      
        1 ccgtccgctt acgatgatgc agaatgcctg ttccagggtg ccgttcagca tcagttgtcc
       61 gatggcttta tctactgcgt tatgtctgcc aatgtcttct ttgatgacga gtaaatttcc
      121 agcctcatcg aagatggcag aagcatgtga accaccggaa ctgtcgaaag tattctggtg
      181 ttcccgcatg gtccggaaca tctgctgtac acgttcggcc gaaggagatt cgtgtacagg
      241 aatatccaca ccggtcagca acaattcggc ttccgtctta ccgcacatac cgcaactgga
      301 aaccgacacc aaagtgcgat tcacttttcc ttctgtgcgg aatgcattct tatctccact
      361 gatattcacc gctgtgatat aaccggcatc attcgcctcg gtgattttga atgtcggatt
      421 ctctttttct gtatagacct gttcagataa taacaagcca cgggccaggg cttcttcatc
      481 gcccggcgtg tgcatggtga tggtgtaggg ctcgccgttc acatagatgc tcagcatgac
      541 ctctacagtg agggggtctt ccacaggttg cgcagttccc tgcaccacct tgtgggcgtt
      601 gtatctgcga acgggtttct gttccatggt attggaacaa tgaaggtaag tgatttagtt
      661 catggagctc cgtatcagta gggtccgaga aagtaggtat caatgtgata ggtttccccg
      721 aaaccggttg tgatgagtac agcatcacat agagttacgg aataggaacc gtcaccatag
      781 ttgttcaggt atatcttttc attcaattgc aggtaaccga acaggggacc cccttgtgtg
      841 acggaaacat aaaccttctc cggatcgctc gggaacattg tgctgccgga gctggaatag
      901 atacctgatg atggttcttc actgaacctg aaatggatgg acatcaatgt tccgctgact
      961 gaagccgtga tctcccaatc gccgcctgca tcattcagag ccgatgcata atagacatta
     1021 taggagctgg caaatgcatc cagataaaaa tagtttgtgt caaatgagca gggtgcgatc
     1081 acttcattgg taatgtcggc ggttgaaatc ggttcacctt ttgcgtatcc ctgtttattg
     1141 gatgcccatg cattgaaata gtaaacggta ttgttctcaa agattgcgct ttcaagttcc
     1201 agtttgaaga atgagccata ggtggtcacc tccaactggt tctccaggat gctgaactct
     1261 tcattttcgc tgtagcagaa accggcatgc tgtatatccg tgaacccttc atcaagtata
     1321 tgtccgtaaa ggctgatatt tccgtcatcg ttgatgacca cagagtccat actgaccacc
     1381 ggcaggtaat cctccacgtt ttcaaaaggc ttgatacacg atgacaccat agagaacatg
     1441 gccacctgaa aaagaatgat atgtgtgttg tttattgcca tcaatagtag tatccgatcc
     1501 tgattcccag accataatag tccacgaatt cgtttgtgtc aaaattgtag ttcaatgagc
     1561 gttcaaagag caatcctgct aacataaatt cattgaattc gtattctact cccagcctgc
     1621 cggaaattcc accaagatat tccggcccgg tataactcag agatggggca gctgtaccac
     1681 catagtaatc tcttgtataa ctcatgctga accagccgaa acgggcacct atgcctccga
     1741 atccaaacag atcgatgccc gggggctggt aggggcagcc gtcaacaaac acggaaagtc
     1801 cgaacaacat agctctgtcg atcaccagaa ttcccggaac cacctcgtcg taattcagat
     1861 catcaaagta aacgatattg aatgtgtctg acctgacaga caaaggtgaa aattcaaatg
     1921 attgtccgat cctcagataa tctgattcgg tgaaagagct ttgattgaac agggtgaatt
     1981 gccaggaatt gccatagaag tatgagatat tcgacagggg ttggatccgg ccaacctgaa
     2041 gtccagcaaa ttgggcatgt gcatgtgacc ccatcatgat caacagtatg catatcagtt
     2101 gccgggccct ggagttcata aatttttatt taactgattc agattattta gatcagctca
     2161 gtccggtgat cttgccgttg tcgtcaatgt ccatgccttc actggcagga tgctccggca
     2221 atcccggcat gcgcatcatg tcgcccagaa tgggaatgac gaaaccggca cccgaagcga
     2281 actcgaactc acgaacggtg acactgaatc ctgttgggcg accaacgatc ttttcgttat
     2341 cgctcagcga cttttgcgtc ttggccatgc aaaccggcag gttgtcaaaa cccaggttct
     2401 tgatggtctt cagttgcagc aatgccttgg aagagaagtt gacatcatcg gcaccgtaga
     2461 tctctttcgc aatggtgcgg atcttgtctt ccaccggcag gttccagtcg tacaaacgat
     2521 ggaatttgtt cgcaccactt tctatcatat ctaccacagc ctgagccaga ttcatggtgc
     2581 cctcaccgcc tttggcgaag ccttcactga ccacagcttt ggcacccagt ctggcacagg
     2641 catcaatgac gaactgtact tcttccgaac tatcagccgg gaaggcattg atggcaacca
     2701 caggtgtcag gccatactta ccgcagttct cgatatgctt ctgcaggttc tcgaaacctt
     2761 tctgcacccg ctccaggctg gcggtgttgt actcttcttt ctttgctccg ccatggtgac
     2821 gaagggcacg gatggtggct acgatcacga tggcatcggg accgaaaccg gcgtatccgc
     2881 acttgatgtt catgaatttc tctgcaccga ggtcggcgcc gaaaccggct tctgtgacca
     2941 cataatctcc taaggacaaa cccatcttgg tagcaagtat ggtattggtt ccctgtgcaa
     3001 tattggcgaa cggtccgccg tggatgatgg caggattgcc ttccagtgtc tgcaccaggt
     3061 tgggcttgat ggcatctttc agcaggatgg ccatagcaga ttctgctttc aggtcgcggg
     3121 caaagaccgg tttcctgtcg taggtctgtc cgatataaat atttcccaaa cgttgtttc
//
LOCUS       DAOMAX010000291         3176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6210_length_3176_cov_4.101570, whole genome shotgun sequence.
ACCESSION   DAOMAX010000291 DAOMAX010000000
VERSION     DAOMAX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6210_length_3176_cov_4.101570"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PK742_11795"
     CDS             <1..930
                     /locus_tag="PK742_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQU77316.1"
                     /translation="VKVFNNGSTVPSNTFYLTGMIASEDVYLIVNASADSALLSLADT
                     TASVTFFNGNDAVVLFQFMDTLDMIGVVGEDPITEWPVDTGGTGENTLVRKASVSAGQ
                     TDWSIGATEWDVYAQNTFDFYGTHTADGCAPACTTPDDITVEPDAGGTTALVSWTAVD
                     GGTQYGVRYREIGSPMWLKKATTGSMTMLTGLTPGADYEFQMVTRCGEDRAGPTSLMF
                     FSTPARTGIFTEGVSVFPNPSSGTFYMNGFDNETEELQIHVYDLSGRLLYLRSDMHAG
                     ELVAIDLPDALTGQMILEAIKGDQMIRSMIIVE"
     gene            1015..>3176
                     /locus_tag="PK742_11800"
     CDS             1015..>3176
                     /locus_tag="PK742_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77317.1"
                     /translation="MKPHITLSLLAAVSCWFSSVHAQEVQNSSPAIGVSPLQSESRSG
                     VLELMAQPEQSSSTRTGLEFIPIGTAYNLYTTLNNSPSQVACLPELNTVVFIHRHNAG
                     TTGGSGGLSFDSSTDGGATWTINQSLTPSYNAGAEPEVIGGRYPSITLWNPPGNTLTD
                     NVFVLAHGPELDPAAGGSWGSSFHISTAMDMDYLNETYYSTDDAHEDYLTGGLMTYND
                     GSIWDLKTFVPGNHFSTVKHVFHPPSKTFIRNEHVFEPDWYVSDGELVVANGEGETSF
                     DPTGTIGYAVLLGCLSDDVVKTMAPVIYKTTDAGATWNQLPSFDWTAMPLMEEWMFAT
                     VDAGEYIPYFEQVSATVDVTGRLHIFSEVFSRYTSNYADSIMFIYSNSNVFLHLSTSD
                     GTDWQGEALGYTTLDKAFIGSAEIQNQTQISRTPDGSKLFFCWNVSDSLMAYVNDRPN
                     IYVKGYDVVSNTFTETVNLTMGTAFVFDGFFPTTAGVTLDNGFGEYELPIVFAVPGAT
                     DLSPPQFYYIDGVTFTDADFVPAEPATAAFTYFVDGPTVLFNNNSLFADTYAWDFGDG
                     YSSTIEDPVHTYADPGVYNVCLTASNDLSTDEFCQDVTVVLDCEPPSPLVVLLVSSNQ
                     IRLGWSEVPGADYYQVQYRQAGTAAWSKKTTITLNVKLTGLTPGTTYEYRARSNCAGS
                     FSSYSPIESVATTMRLGSTDPADPAIYPNPTTGSFYIQT"
BASE COUNT          818 a          743 c          749 g          866 t
ORIGIN      
        1 gtaaaagtgt tcaataacgg aagtaccgtg ccttccaata ccttttacct gacaggtatg
       61 atcgcctctg aagatgtgta cctgatcgtg aacgccagcg ctgattctgc attgctcagc
      121 ctggcagata ccactgcctc cgttacattc ttcaatggca atgatgcggt ggtactcttt
      181 cagtttatgg atacattaga tatgatcggt gtagttggag aagatcctat cactgaatgg
      241 ccggtggata caggaggaac aggtgagaat acgctggttc ggaaggcttc agtttctgcc
      301 ggacaaactg actggagcat aggtgccacg gaatgggatg tatatgcaca gaatactttt
      361 gatttttacg gcactcatac agcagatggt tgtgcacctg catgtactac ccccgatgat
      421 attactgtgg aacctgatgc cggaggtact actgcattgg tttcctggac cgctgttgac
      481 ggaggcacac agtatggtgt acgttaccgt gaaattggat cgcccatgtg gctgaagaaa
      541 gcaacaacag gctccatgac catgctgacc ggtttgacac caggtgccga ttatgagttc
      601 cagatggtta cccgctgcgg tgaagaccgt gcaggcccaa cctctctgat gttcttcagc
      661 accccggcaa gaaccggcat atttacggaa ggagtgtcgg tatttcccaa tccttcatca
      721 ggaaccttct atatgaacgg tttcgataac gaaacagaag aattgcaaat acatgtatat
      781 gatctgagtg gtcgtttgct ctatcttcgt tctgatatgc atgcaggaga actggtagca
      841 attgatctgc ctgatgcctt gacaggccag atgatcctgg aagctatcaa aggagatcag
      901 atgatcagaa gtatgataat tgtagaataa tgaactttca tgttctaaaa aaggagcaga
      961 tatctgctcc tttttttatt tttattccat tcagactttt ttgaagtaga tcctatgaaa
     1021 ccacacatta ccctttccct gctggctgct gtcagttgct ggtttagctc tgttcatgcc
     1081 caggaggtcc agaactcatc accagccatt ggtgtcagcc ctctgcagag tgaaagcaga
     1141 tcaggcgtgc tggaactgat ggcacaaccg gaacaatctt cttcaacccg gaccgggttg
     1201 gagtttattc ctatcggcac agcttataat ctgtacacca cccttaataa tagtccgtca
     1261 caagttgcat gtcttcctga gctgaataca gttgtattca tacacagaca taatgcgggt
     1321 actaccggag gcagcggagg cttatcattt gacagcagca ctgacggtgg tgcaacatgg
     1381 accattaacc aaagccttac accttcttat aatgcaggag ctgagcctga agtaatagga
     1441 ggaagatacc cgtccatcac actgtggaat ccaccgggaa ataccttgac tgataatgtc
     1501 ttcgttctgg ctcatggccc tgaactggat cctgctgccg gtggttcatg gggaagctct
     1561 ttccatattt ctacagcaat ggatatggat tacctgaatg aaacctatta ttctacggat
     1621 gatgctcatg aagattacct gacaggtggc ctgatgacct acaatgatgg cagcatctgg
     1681 gatctgaaga catttgtacc cggcaatcat ttcagcactg ttaaacatgt gttccacccg
     1741 ccttccaaga cttttataag aaacgaacat gtctttgaac cagactggta tgtgagtgac
     1801 ggtgagctgg tagtggctaa tggtgaaggt gagaccagtt ttgatcctac cggtacgatc
     1861 ggatatgcgg tccttctcgg ttgcctctca gatgatgtgg tcaaaaccat ggcacctgtc
     1921 atttataaga ccacagatgc aggagctaca tggaatcagt tgccatcctt tgactggacc
     1981 gccatgccac taatggaaga atggatgttt gcaaccgtag atgccggaga atacataccc
     2041 tattttgaac aggtttcagc tacagttgat gtaacaggtc gtctgcatat tttttctgag
     2101 gtattcagca gatataccag caattatgca gattctatca tgtttatcta cagcaattcc
     2161 aatgtctttt tgcatctcag caccagcgat ggaacggact ggcagggaga agcattgggc
     2221 tatactacac tggataaggc atttatcggt tcagctgaaa ttcagaacca gactcagata
     2281 tccagaacac ctgatggcag caaacttttt ttctgctgga atgtaagtga ttcactcatg
     2341 gcttacgtaa atgaccgccc taatatctat gtgaaagggt atgatgtggt ctccaatacc
     2401 tttacggaga ccgtgaatct tacaatggga actgcctttg tttttgatgg ttttttcccc
     2461 accacagccg gtgttaccct ggataatggt tttggtgagt acgagcttcc catagtattt
     2521 gccgttcctg gcgccacaga tctgtccccg ccacaattct attatattga cggggtgacc
     2581 tttactgatg cagattttgt tcctgctgaa cccgcaacag ctgcctttac ctattttgta
     2641 gatggcccta cagtgctgtt caacaacaac tcactgtttg cagataccta tgcatgggat
     2701 ttcggcgacg gatattccag caccatagaa gaccccgtgc atacctatgc agaccccggt
     2761 gtgtacaatg tctgtctgac tgcctccaat gacctgagta cagatgagtt ttgtcaggat
     2821 gtaacggtag tactcgactg cgagccccct tcacccttgg tggtactgct ggtcagctcc
     2881 aatcagatca gactggggtg gtcagaagtg ccgggcgcag attactacca ggtgcagtac
     2941 agacaagccg gaactgctgc atggtcaaag aagaccacca tcactttgaa tgtgaaactt
     3001 accgggctga cacccggtac tacctatgag tacagggcaa gaagtaactg cgctggttca
     3061 ttcagcagct attctcccat tgaatcagtt gctactacca tgcgcctggg aagcactgat
     3121 ccggcagatc cggctattta ccccaatccc acaaccggta gtttttatat tcagac
//
LOCUS       DAOMAX010000292         3163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6258_length_3163_cov_3.607465, whole genome shotgun sequence.
ACCESSION   DAOMAX010000292 DAOMAX010000000
VERSION     DAOMAX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6258_length_3163_cov_3.607465"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1628)
                     /locus_tag="PK742_11805"
     CDS             complement(189..1628)
                     /locus_tag="PK742_11805"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQU77318.1"
                     /translation="MLFPVSLLQAQKIAQHDALTVEELQADLDVFLDVYDHHPGLHLY
                     HTLEETDSVKAALQHLIQNGTSTEVFYTELLRALTFLGDGHTGISPGAVYGETFPGNR
                     IIPFGFRILRDTVYISALKTGSYSECLFSRLLSVNGVDADSLLRHMYALTCADNQNHI
                     FKQAFTERTFGRNLSYFFGATACYEMVCLSPAGDTIITQVNSVSKQDLPVSPSVPPLH
                     SVIDSEHDLAILTINTFQYPLLVDAGTDFHSFLDKFFKKVRRKKIGHVVIDLRENYGG
                     SSVLALSVFAYLSPEDFRYMDHSFTSLDGSESFARFTPYPDGHYPFFNTHDTTAAGNR
                     LLVSNGEDSKEIFSANTIPFGPKKKLRDITANKYDGKLYVLTSGMTFSSASVLTAKCA
                     ERPNTTLIGEPTGSAAGVFCGGGFLYVSLPHSGFRVELPYMERHVALTDPDTDPHLPV
                     MPDHWIDIHQADLMEGRDPVLEKVYELIH"
     gene            complement(1733..2851)
                     /locus_tag="PK742_11810"
     CDS             complement(1733..2851)
                     /locus_tag="PK742_11810"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQU77319.1"
                     /translation="MNRIQQMSWMILLLAAGCTFSGKVVRLPVTDGLEHTALSAEQTG
                     MITSTLAQFPDHSQIAVAVLTGNETLYFGAIRQHDTIRPCNNANSVFEIGSITKVFTT
                     QLLMDAVRSGKIKSLDEPVASYLNYSIKGQPDITFRQLASHTSGLPGNLSASIFTTDA
                     ANPYKKWDEEKLRDFLQHDVAMHDAPGQNYLYSNVGMALLATILCSVSERDYEFLLQE
                     NICDPLHMDHTTTNRDQISSDLVQGYNWKGKPTENWDMDAMAGAGAILSTVSDLAIYA
                     GWSMTALHQSLIDMTLPTVQINDDLQVALGWHVVSNKTPEPFLWHNGGTGGYKSSMAI
                     DLNNGRSVVILTNIGATDNPRKGLIDQLCFDLVNSFEG"
BASE COUNT          794 a          739 c          798 g          832 t
ORIGIN      
        1 gattcctgat tgcctccttc gtcggcttca ggaatgacgt attcctttgg aaagggaatt
       61 agtaggcagt tggcgagttg gcgaattgac atattgtttg ttggaattcg ctacgtacgc
      121 tttgtgatcg tggcgtcgca gaggttaaca tgtaacactt tgtcaaggct actacagaaa
      181 aaccctgctc aatgtatcag ttcatagacc ttttccagta cagggtcacg gccttccatg
      241 agatcagcct gatggatgtc tatccaatga tccggcatga ccggcaggtg cggatcagta
      301 tcgggatccg taagggccac atgccgttcc atataaggca gctccacccg gaatccggaa
      361 tgcggcaggg agacgtagag aaatcctcct ccgcaaaaga ctcctgctgc actgccggtg
      421 ggttcgccta tgagggtagt gtttggcctt tcggcgcatt tggctgtcag cactgatgcg
      481 gacgaaaatg tcataccact ggtaagcaca tacaactttc catcatattt attggcagtg
      541 atatcccgga gctttttctt cggcccgaac gggatggtat tggcagaaaa gatctctttg
      601 gaatcttcgc cgttggaaac cagcaaccgg tttcccgcag cggtagtgtc atgggtattg
      661 aaaaaaggat aatgcccgtc tggatatgga gtaaaccggg cgaagctttc tgagccatcc
      721 agcgaggtga aactgtggtc catatacctg aaatcttcgg gagagagata tgcaaacacg
      781 ctcagggcaa gcacactgct tccgccataa ttttccctca ggtcgatcac cacatgtccg
      841 atcttctttc tgcgcacctt tttaaagaac ttatccagga aggaatgaaa gtctgttccg
      901 gcatccacca gcaagggata ctgaaaggtg ttgatggtca gaatggccag gtcgtgttcg
      961 gaatctatca cactatgcag cggtgggacg gaaggagaca ctggcaggtc ttgcttactg
     1021 acgctgttta cctgggtgat aatggtatcg ccggcgggcg ataaacagac catttcatag
     1081 catgcagttg ctccaaagaa gtaactgaga ttcctgccga acgtgcgctc tgtgaatgcc
     1141 tgtttaaata tatgattttg gttatcggca caggtgagcg catacatatg gcgaaggagt
     1201 gaatcggcat caactccatt gaccgatagc aggcgtgaga agaggcattc ggaatagctt
     1261 cctgttttca gggcagagat gtacaccgta tcacgcagaa tacgaaaccc gaaggggatg
     1321 atccggttac cggggaacgt ttcaccgtac acagcaccgg gtgagatgcc tgtgtgcccg
     1381 tcgcccagaa aggtcagtgc ccgcagcaat tcggtataga atacttcggt tgatgtccca
     1441 ttctgaatga ggtgctgcaa ggcagctttc acagaatctg tttcctccag ggtatgatac
     1501 aggtgcagac cagggtggtg gtcataaaca tccagaaaga catccaggtc ggcctgcagt
     1561 tcttcaacgg tcagggcatc atgctgtgca attttctgtg cctgcaacag gcttacagga
     1621 aacaacatca accataaagc tgctgatctc agaaaaccga ttgccaggtg tggagttgta
     1681 tttatagtat tagtcagacg cattctgttt atcaaattcc tttcagaaaa tcctatcctt
     1741 caaaagaatt caccagatca aaacaaagct gatcaataag tcctttccgc ggattgtcag
     1801 tagcgcctat attggtcagt atgaccacac tcctgccgtt attcagatca atggccatgg
     1861 atgatttata gcctcctgta cctccattgt gccagaggaa tggttccggt gttttattgg
     1921 acaccacgtg ccaacccagt gctacctgca ggtcatcgtt gatctgtaca gttggcaatg
     1981 tcatgtcaat tagtgactga tgaagggcag tcatgctcca acctgcatag attgcaagat
     2041 cgctgacggt tgaaagtatt gcaccagcac ctgccatggc atccatatcc cagttctctg
     2101 ttggttttcc cttccagtta tagccctgca caagatcact gctgatctga tcccggtttg
     2161 tagtggtatg atccatatgc aggggatcgc agatattttc ctgcagcagg aactcataat
     2221 ctctttctga cacactgcac agaatagttg ccagcaaggc catacccaca ttggaataca
     2281 gataattttg gcccggtgcg tcatgcatgg caacatcatg ctgaagaaag tcacgtagtt
     2341 tttcctcatc ccatttctta tacgggtttg cagcatctgt tgtaaaaata ctggcactaa
     2401 gatttccggg aagcccggag gtgtggctgg ccagttgcct gaaggtgatg tcaggttgcc
     2461 ctttgatact ataattcagg taagaagcaa caggctcatc cagacttttg attttgccgg
     2521 atctgacggc atccatcagt aactgggtag taaacacctt tgtgatgctg ccgatctcaa
     2581 atacactatt tgcattgttg catggacgaa tggtgtcgtg ctgacggatc gcaccaaaat
     2641 acaaagtctc attaccggta agcacagcaa ctgcgatctg tgaatgatca ggaaattgtg
     2701 ctaatgtgga agtgatcata ccggtttgct ctgcagaaag agctgtatgc tccaagccat
     2761 ctgttactgg caacctcacc acctttccgg agaaagtaca tcctgctgca agcagcaata
     2821 tcatccagct catctgctgt attctgttca tatctgtctg ccttttgttt accgggcctc
     2881 cggatggtct aaaataaaca aacgggagca ttcgccgcga tagctacagg ctcaccagcg
     2941 aattggagaa ttggttggtc actccctccg gatagttatc aggagctatg gtgatcaaca
     3001 gctggcgaat tgaaaaattg tttgtctgta tgcgctgcga ccgctgcggg atggtggcat
     3061 cgctgcgctt aaatgtaccg gagttcctct ggaaggttta tcctggtctt gaagtatgga
     3121 ctggtgacgg caactctgtc gggaaggtgg gtgcatagtg ctt
//
LOCUS       DAOMAX010000293         3158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6272_length_3158_cov_4.724460, whole genome shotgun sequence.
ACCESSION   DAOMAX010000293 DAOMAX010000000
VERSION     DAOMAX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6272_length_3158_cov_4.724460"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="PK742_11815"
     CDS             <1..1646
                     /locus_tag="PK742_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQU77320.1"
                     /translation="DGETLEVNMQLYSSQAELDEVVVVSEKESRKVRAENVMPAMTLS
                     THDASGAVSNYSYAPLMNEEVYVEGHNTEEYDEINEIGFRKVIGNPLSTFSIDVDNAS
                     YSNIRRFITYNQVPQKDVVRIEEMINYFDYSYPQPTGVDPFSLNTEMSECPWNPDTRL
                     IMIGIQGKDIDYSKADPSNLVFLLDVSGSMGDQNKLPLVKKSLYKLIDQLSEKDRIAI
                     VVYAGAAGLVLPSTPANDKIAIKKALDNLQAGGSTAGGQGIELAYKTAADNLIRNGNN
                     RVILCTDGDFNVGASSDAEMVRLIEEKRRSGVFLTICGFGMGNYKDSKMEKIANNGNG
                     NYFYIDNEREAEKVFVTDMRGTLFTIAKDVKIQVEFNPAKVEEYRLLGYENRMLNKED
                     FDDDKKDAGELGAGHRVTAIYEVKLADNRSFSPQGMPEGETLKYQQTKVNETAYYSEE
                     IMSLRMRYKKPDEDVSRLIETAVLDKQVPLSQTSDNYRFAVAVTEFGLLLRDSEFKGD
                     AKYEEVLALAKGAKGKDMHGYRAEFIGMVEQFLTFASTK"
     gene            1876..3144
                     /gene="purD"
                     /locus_tag="PK742_11820"
     CDS             1876..3144
                     /gene="purD"
                     /locus_tag="PK742_11820"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQU77321.1"
                     /translation="MNILLLGSGGREHAMAWKIAQSPLCKYLYIAPGNPGTAECGQNV
                     PIAVRDFNAQVDFCRENKVDLVVVGPEDPLVLGVVDHFAEHLPTVPVIGPPAEGAQLE
                     GSKAFAKEFMQRHAIPTAAYRSFTVAEKAEGLAYLQQHDLPLVLKADGLAAGKGVLIC
                     QTLEEATEGFIAMLDGMFGDASSRVVVEAFLEGIELTVIVLTDGQSWKVLPPSKDYKR
                     IGEGDTGLNTGGMGAVSPPPFATPAFMTTVEEQVIAPTIKGLRTDGIAYKGFLYFGLI
                     KVGDAPFVIEYNCRMGDPETQVIMPRIFSDIIPLYLSVARGDLAEAEFETDPRAAATV
                     VLASSGYPGSFEKGLEITGCEHLEDCMLFHAGTVSDDQGILYTNGGRVLALSCYGTDL
                     PEALRNCYTNTARIYFGNRYFRRDIGWDVV"
BASE COUNT          837 a          842 c          836 g          643 t
ORIGIN      
        1 aggacggcga gacccttgaa gtgaatatgc aactttattc cagccaggca gaactagacg
       61 aggtcgttgt ggtatcagag aaagaaagcc gtaaggtcag agcagaaaat gtcatgccgg
      121 ccatgacact tagcacacat gatgccagcg gagctgtttc caattacagt tacgcaccat
      181 taatgaacga agaggtctat gtagaaggac ataacaccga agaatacgat gagatcaatg
      241 agatcggttt ccgcaaggtc atcggcaatc cgctcagcac tttcagtatt gacgtggaca
      301 atgcatctta ttccaacatc cgccgcttca tcacttacaa ccaggtaccc cagaaagatg
      361 tggtgcgcat tgaagagatg atcaactatt ttgactacag ctatccgcag ccaaccggtg
      421 tggatccttt tagcctcaac acagagatgt ctgaatgtcc ctggaaccct gatacccgtc
      481 tcatcatgat aggtattcag ggaaaagaca ttgactattc caaagctgat ccttccaacc
      541 tggtgttcct cctggatgtg tcgggctcca tgggagatca gaataaactg ccgcttgtta
      601 aaaaaagcct gtacaaactc attgaccagc tgagcgaaaa agaccgaatt gccatcgtgg
      661 tatatgccgg tgcggcagga ctggtgctgc catctacccc ggccaatgac aagatcgcta
      721 tcaagaaagc actggacaac ctgcaggcag gcggctccac tgccggcgga caaggaatag
      781 aactggccta caaaaccgcc gcagataacc tgataagaaa tggcaacaac cgggtgatct
      841 tgtgtacaga cggcgatttc aatgtgggcg cttccagcga tgcagagatg gtccgcctca
      901 ttgaagagaa aagaagatca ggtgtgttcc tgaccatctg cggcttcgga atgggcaact
      961 acaaagacag caagatggag aagatcgcca acaacggaaa tggcaattac ttctacatcg
     1021 acaatgaaag agaagcagaa aaagtgttcg tgacagatat gcggggtacc ctgttcacca
     1081 tcgccaagga tgtgaagatc caggtggaat tcaatcccgc caaagtggaa gaataccgcc
     1141 tcctgggtta tgagaaccgc atgctgaaca aagaagattt cgacgacgac aaaaaagacg
     1201 caggtgagct gggtgcaggc catcgggtca cagcaatcta cgaagtgaag ctggcagaca
     1261 accgcagctt ttcacctcag ggtatgccgg aaggcgaaac gctgaaatac cagcagacca
     1321 aggtcaacga gaccgcatac tacagtgaag agatcatgag tctgcgcatg cgctacaaga
     1381 agcccgatga agatgtgagc cgcctgatcg aaactgctgt cctggataaa caagtgccgc
     1441 tcagccagac atccgataac tatcgctttg ctgtggctgt gacagagttc ggattgctgc
     1501 ttcgtgacag cgaattcaaa ggtgatgcca aatacgaaga agtgcttgcc ctggccaaag
     1561 gtgccaaagg gaaagatatg cacgggtaca gagctgaatt catcggcatg gtggaacagt
     1621 tcctgacttt tgcgagcaca aaatgacgtt tccgttttca tgtatgttgg cccgctgcct
     1681 gtacaggtgg cgggcatttt tttgtcatct atgaaaacag gatgaagggg gaataaaaag
     1741 cacaggtatc tgccgagaat ggatgatgca tgtctgacca aaggctaacc aaaacagctt
     1801 tctgtctgat atctctgaac acaccataaa aggtcaactg accatctctt cctattttta
     1861 cccgaaaatc tacacatgaa cattctgctg cttggatccg gaggaagaga acatgccatg
     1921 gcctggaaga tcgcccagtc gccgctgtgt aaatacctct acatcgcccc cggtaatccc
     1981 ggcactgctg aatgcggaca gaatgtgccc atcgccgttc gcgatttcaa tgcccaggtg
     2041 gatttctgcc gggaaaacaa ggtggacctc gtcgtggtgg gacccgaaga cccgctggtg
     2101 ctcggtgtgg ttgatcattt tgccgaacac ttgcccactg tacctgttat cggtccgcct
     2161 gccgaaggtg cacaactgga aggctccaag gccttcgcca aggagttcat gcagcgacat
     2221 gccattccca ccgcagcata caggtcattc acagtagctg agaaagccga aggactggcc
     2281 tacctgcaac agcatgacct ccccctggtg ctcaaggccg acggactggc cgcaggaaaa
     2341 ggcgtgctca tctgccagac cctggaagaa gctaccgaag ggttcatcgc catgctcgac
     2401 ggcatgttcg gcgatgcatc cagcagggtg gtggtggaag ctttcctgga aggcatcgag
     2461 ctgaccgtca tcgtgctcac cgacggccag agttggaagg tcttaccgcc ctctaaagac
     2521 tataagcgca tcggcgaagg agataccggc ctcaacaccg gcggtatggg ggccgttagc
     2581 ccgccgccct ttgccactcc ggccttcatg acaacggtgg aggagcaggt catagcgccc
     2641 actatcaaag gtctgcgtac tgatggcatc gcctacaagg gttttctcta tttcggactc
     2701 atcaaggtgg gcgacgcccc ctttgtaata gaatacaact gccgcatggg cgatccggag
     2761 acccaggtca tcatgccccg catcttcagc gacatcatcc ccctctacct cagcgtggcc
     2821 cgcggcgacc tggccgaagc cgagttcgag acagaccccc gggcggcagc caccgtggta
     2881 ctggcctcca gcggctatcc gggcagtttt gagaaaggcc tggagatcac cggctgcgaa
     2941 cacctggaag actgcatgct cttccacgcc ggcacggtat cagatgacca gggcatcctc
     3001 tacaccaacg gcggccgggt gctggccctg agctgctacg gcaccgatct gcccgaagcc
     3061 ctgcgcaact gctacaccaa caccgcccgc atctatttcg gcaaccgata cttccggaga
     3121 gatatcggtt gggatgtggt gtgaaatccc ctatatag
//
LOCUS       DAOMAX010000294         3158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6274_length_3158_cov_3.269417, whole genome shotgun sequence.
ACCESSION   DAOMAX010000294 DAOMAX010000000
VERSION     DAOMAX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6274_length_3158_cov_3.269417"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="PK742_11825"
     CDS             complement(<1..1995)
                     /locus_tag="PK742_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU77322.1"
                     /translation="MGNFCRFGWILLLLSGLSAAGQNTVIIEGVVRDGADKSPMFGVA
                     VINTAGEGVLTNDEGRFSIQARVPDTLLFRYVAHVPVKIAVDGPMKPLDIVLQEDGGL
                     LSTVVVTGSKFEKEIGEETVSIEVIRSSLIDHANNVSIEQSVQKVPGVNVIDGQANIR
                     GGTGYSYGAGSRVLLLMDDIPLLTADAALSNWNFVPVENIAQIEVIKGASSALYGSSA
                     LNGIINVRTAYPGSEPYTRITSFGGIYEGPSDKTQQWWSKEQPYFSGMSFAHRQKFGK
                     FDLVAGGYGFVQDSYLEGDYDRFGRGNIQTRYRLNERLSFGVNGVLQINRSASFFFWA
                     DSAHLFSPAENTMTFNKGNRMSLDPFIHYRDPWGNQHKLQGRYYKSDNRNESDQSSLA
                     DLYYAEYQFQKKLEAVPVVVTAGATMNNGVVQAELYGDSSYVAANQALYLQADWSPGR
                     LNLNGGFRWEHNHLDLLDESRPIFRFGASYRAAEYTFIRASWGQGYRFPSIAEKFVST
                     SVSLFNIFPNPELESETGWSAELGLKQGLQISGWQAYVDASVFMQRYFNMMEFTFGYY
                     PSPGVIFPYGFKSLNIGNTEIRGGEVSVSGAGKFGRFPLSVLAGYTYIDPVFQDFDSL
                     TDANSTADYNILKYRFKHVVKFDAESSFEHFRPGIEVQYNS"
     gene            1998..>3158
                     /locus_tag="PK742_11830"
     CDS             1998..>3158
                     /locus_tag="PK742_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQU77323.1"
                     /translation="MKHPKIAVPLIRQKNNQVMSDEPSNLPFYEVVNRQRQFFRQANT
                     LHYNQRINILKDLLQVVKENEQAILTALAKDLHKSTFEAWAVEYGVIMEELRLAIRHL
                     REWMQPKRLKTPLLHFRANSSVLPVPFGHVLIIAPWNYPFMLALRPAIGAIAAGNAVL
                     IKPADAASATEALLENMINHQLKTPWIQVAKAGPEESARLMEHRFDFVFFTGGTEIGR
                     KVYQAAAQHLTPVVLELGGKNPCIVHHDADLTIAARRIAWGKFSNGGQTCASPDYLLI
                     HESVYEQFIQKLIQQIRIFYGDDPQHHPDYGRIINKRHWERIMKLIPGAEVLAGGTGN
                     EADLYIAPTIIRPKDTAHPSMQEEVFGPVLPIFTYQTINEAYTFIDRHPDPLA"
BASE COUNT          760 a          897 c          763 g          738 t
ORIGIN      
        1 actgttgtac tgcacttcta tgcctggacg aaaatgctcg aaggacgact ctgcgtcaaa
       61 cttcaccaca tgtttgaaac ggtatttcag gatgttgtaa tcggcggtgg aattggcatc
      121 tgtcaggctg tcgaagtcct ggaatacggg atcgatatag gtgtaacccg ctaacacaga
      181 caatgggaat cttccaaatt tcccggcacc tgaaaccgat acctctcctc ccctgatctc
      241 tgtattgccg atgttcagcg acttaaatcc atacgggaat atgacacccg gcgatgggta
      301 ataaccgaag gtgaattcca tcatattgaa ataccgctgc atgaatacgg aggcgtccac
      361 ataagcctgc caccctgata tctgcagtcc ctgtttcaac ccgagctccg cactccagcc
      421 agtttccgat tccagttcgg gattggggaa gatgttaaag aggctgacgc tggtgctgac
      481 gaatttctct gctatggacg ggaagcgata tccttgtccc cagctggccc gaatgaacgt
      541 gtactctgcc gccctgtagc tggcaccaaa tcgaaaaatg ggtctgctct catccagcag
      601 gtccaggtgg ttgtgctccc agcggaatcc gccgttcagg ttcagtcttc ccggcgacca
      661 atccgcctgg agatataagg cctgattggc agccacatag ctggaatcgc cgtataactc
      721 ggcctgtaca actccattgt tcattgtagc tccggcggtg accaccaccg gtacagcttc
      781 cagttttttc tggaactggt actctgcata atacaggtcg gccagcgaac tctggtcaga
      841 ctcgttccgg ttgtctgact tgtaatacct gccctgcagc ttgtgctggt tgccccaggg
      901 atcgcggtaa tggataaaag gatccagcga catccggttt cctttattga aggtcatggt
      961 gttctcagcc ggactgaaga ggtgggcact gtcggcccag aagaagaaac tggcgctgcg
     1021 attgatctgc aatacgccgt tcactccgaa tgataatcgt tcgttcagtc tgtacctggt
     1081 ctggatattt ccccggccga agcggtcata atcgccctcc aggtaactgt cttgcacaaa
     1141 cccgtaccct ccggccacca gatcgaactt tccgaacttc tgccggtggg cgaaagacat
     1201 accggagaaa tacggctgct ccttgctcca ccattgctgg gtcttgtctg acggaccttc
     1261 atatatgcca ccaaacgaag tgatccgggt gtaaggctct gaacccggat aggctgtccg
     1321 cacattgatg atcccgttca atgccgatga accgtacaat gccgaactcg cccctttgat
     1381 gacttctatc tgtgcgatgt tctccaccgg tacgaaattc cagttgctca gagctgcatc
     1441 tgccgtaagc agggggatat catccatcag caacaggacc cggctgccgg ctccatagga
     1501 atagccagta cctccgcgaa tgttcgcctg cccgtcgatg acattgacac ccggcacttt
     1561 ctgtacactc tgttcgatgc tgacattatt ggcgtggtct atcagcgacg accgtatcac
     1621 ttcaatggat actgtttcct ctccgatctc cttctcaaac ttactgccag ttaccactac
     1681 ggttgacagt aaaccaccat cctcctgcaa cactatgtcc agcggcttca tggggccatc
     1741 caccgcgatc tttaccggca catgtgccac gtatctgaac agcagcgtat cgggcaccct
     1801 ggcctgaatg gaaaagcgcc cttcgtcatt ggtcagcacc ccttcgccgg cagtattgat
     1861 gactgccaca ccgaacatcg ggcttttgtc tgcgccatca cgcaccacac cttctatgat
     1921 cacggtgttt tgtcccgcag ccgacaatcc tgacagcaac agcaggatcc atccaaaacg
     1981 acagaaattt cccatatttg aagcatccaa agatagcagt tccgcttatc cgtcaaaaga
     2041 acaatcaggt catgtctgac gagccttcca accttccttt ttatgaagtt gtcaacaggc
     2101 aacgccagtt cttccgccag gcaaatacat tgcattataa tcagaggatc aatattttaa
     2161 aggatttact tcaagtagtt aaggagaatg aacaggccat cctaaccgca ttagccaaag
     2221 acctgcacaa gtccaccttc gaagcctggg ctgtagagta cggtgtcatt atggaggagc
     2281 tccgcctggc catcaggcat ctgcgggaat ggatgcagcc caaaaggcta aagacacctt
     2341 tgctgcactt ccgggcaaat tcatctgtcc tccctgtccc ttttggtcac gtcctgatca
     2401 tagccccctg gaattatccc ttcatgctgg ccctccgccc ggccattggg gccattgcag
     2461 caggaaatgc ggtgctgata aaacccgcgg atgcagcttc agcaacggag gcactcctgg
     2521 aaaacatgat caaccaccag ttgaaaacgc cctggataca ggtggcgaag gctggtccgg
     2581 aagaaagtgc ccggctgatg gaacaccggt tcgactttgt cttcttcacc ggcggaacgg
     2641 agatcgggag gaaggtctat caggcggcag cacaacacct gacaccggtg gtgctggagc
     2701 tggggggaaa gaacccctgc attgtgcacc atgatgccga tctgaccatc gctgcccgca
     2761 ggatcgcctg ggggaagttc agcaatggcg gacaaacctg tgcatctcct gattatctat
     2821 taatacatga gtctgtgtat gaacaattta tacaaaagct gatacagcag atcaggatct
     2881 tttatggcga tgacccgcaa caccatccgg attacggacg catcatcaac aaaagacact
     2941 gggaacgcat catgaaactc attccggggg ctgaggtctt agccggaggc acgggaaacg
     3001 aagccgacct gtacattgca cccaccatta ttcggccgaa ggatacagcc cacccgtcca
     3061 tgcaggaaga ggtgttcgga cctgttctcc cgattttcac ctatcagacg atcaatgagg
     3121 cttacacatt catagaccgc catcccgatc ccctggcc
//
LOCUS       DAOMAX010000295         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6312_length_3148_cov_5.364371, whole genome shotgun sequence.
ACCESSION   DAOMAX010000295 DAOMAX010000000
VERSION     DAOMAX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6312_length_3148_cov_5.364371"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PK742_11835"
     CDS             complement(<1..854)
                     /locus_tag="PK742_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQU77324.1"
                     /translation="MKVFVCCSLMAIASVAWGNGPDSTSLELSLPDEEVNRMDSLIES
                     WFSDNPAIRFAEEEYRSLGFAATDTPRYNPDEITRRLDAIETPIPLTYNSTVQSFIDL
                     YTERRRSKVSEMLTLGQFYFPMIEEELDRRGLPMELKYVAVIESALNTHAVSKAGATG
                     LWQLMYGTGKLLGLRIDSYVDERRDPYLATEAGIEYLSRMYDIYGDWLLSIAAYNCGP
                     GNVNKALRKAGGGNKTFWDIWAYLPRETRSYVPIFIAATYTFEYHREHNILPAEFTYH
                     YDMVDTVMV"
     gene            complement(952..2385)
                     /gene="gatA"
                     /locus_tag="PK742_11840"
     CDS             complement(952..2385)
                     /gene="gatA"
                     /locus_tag="PK742_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQU77325.1"
                     /translation="MSFRYESLPVLQEQLRKQELSCREVVEHYVRQCHDHRHLNAMLE
                     VFEEDALIQADRLDEKLRNDLPLGKLYGMVIAVKDVIVMKGKEATASSAILQGFESLF
                     NATCIDRLLAEDAIIIGRTNCDEFAMGSSNETSIHGPVLNAADETKVPGGSSGGSAVA
                     VQADLCMASLGTDTGGSIRQPASLTGTVGFKPTYGRISRYGLIAYASSFDQCGTFTRN
                     AVDAAMLTEVMAGQDPHDATSSSQPVPDYAALLEPPAKLRIAWYPQTIDNGKLDPEVK
                     QQFHDLLEKLKAAGHTVESVDFTYLDYLVPTYYVLTTAEASSNLSRFSGIHYGHRTEV
                     TADVDEVISRSRSEGFGEEVKRRIMLGTFVLSSGYYDAYYTHAQKVRRIIRNETLDVL
                     SRFDIIITPTTPTTAFAIGEKTTDPIEMYLADIYTVQAPIAGIPAISVPLHTHSNGLP
                     YGMQLMAAPFREDLLLAVTDHLMQHFR"
     gene            complement(2394..2606)
                     /locus_tag="PK742_11845"
     CDS             complement(2394..2606)
                     /locus_tag="PK742_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQU77326.1"
                     /translation="MNSVLLISMPSGMEWIIIIFAILLLFGGKKIPELMKGLGKGIRE
                     FNDAKNNINKELRDGMNEEPKKSEEK"
     gene            complement(2646..>3148)
                     /locus_tag="PK742_11850"
     CDS             complement(2646..>3148)
                     /locus_tag="PK742_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQU77327.1"
                     /translation="ESGTSTTSTVKEEKKTTAPVNAGLTPEMALIGQNFASNKGRLPW
                     PVERGTITGKFGTQPHAVLKNVTVENNGIDITTTAGAAVRCLFDGEVVNIIFNPSFQK
                     GVIIKHGEYYTVYTGLTSVSVKAGDKVSTKQTIGTAYTDDDRTEVHLEIWKNTTLLDP
                     AAWISK"
BASE COUNT          731 a          761 c          816 g          840 t
ORIGIN      
        1 accatcactg tgtccaccat atcatagtga taggtaaatt cagccggcag gatgttgtgt
       61 tctctgtggt attcgaaagt ataggtagct gcaatgaaaa tgggcacata agacctggtc
      121 tctcttggca gataagccca tatatcccag aaggtcttgt ttccaccccc ggctttgcga
      181 agtgctttgt tcacattacc cggtccgcag ttataggccg caatagataa taaccagtcg
      241 ccgtaaatgt catacatacg gctcaggtat tctatgccgg cttcggtggc caggtaagga
      301 tctctccttt catctacata gctgtcaatg cgaagtccca gcaacttgcc ggttccgtac
      361 atcagctgcc agagaccggt tgcgcctgct ttggaaactg cgtgggtatt gagagcagat
      421 tcgataactg ccacatattt aagttccata gggagccccc gacgatcgag ttcttcttcg
      481 atcatgggaa aatagaactg cccgagggtg agcatctccg ataccttgct gcggcgtcgc
      541 tcggtataga gatcaatgaa ggattgaacg gtgctgttat aggtaagcgg aataggtgtt
      601 tcaatagcat cgaggcgccg ggtgatctca tccggattgt aacgcggcgt atcggtagcg
      661 gcaaacccta aagagcggta ctcttcttca gcaaaacgta tggcagggtt atcagagaac
      721 cagctctcga tcagactgtc catccggttc acttcttcat caggtaagga cagttccagg
      781 ctggtggaat ccggtccgtt tccccaggca acagaggcaa tggccattaa actacaacaa
      841 acgaatactt tcatatctgg catgtgcata gtcacaaaga aatcgatttt taaaactact
      901 acagagtggt tttattttct gagcgaccta aattaagctt tccacaatca attatctgaa
      961 atgctgcatc aggtggtcag tcacagcgag cagcaggtct tcacgaaatg gagctgccat
     1021 cagctgcata ccgtatggca gtccgttact gtgtgtatgc aggggaacag atatggcagg
     1081 aatgccggca atgggggcct gcacggtata gatatctgcg aggtacattt caatggggtc
     1141 tgtggtcttt tcgccgatag cgaatgcggt agtaggtgtg gttggggtga tgatgatatc
     1201 aaacctggag agcacatcga gggtctcatt gcggatgatc cttctcactt tttgcgcatg
     1261 tgtgtaatag gcatcataat agcccgagct gagtacaaat gtgcccagca tgatgcgccg
     1321 tttgacctct tcaccaaatc cttcgctgcg actgcggctg atgacctcat ccacatcggc
     1381 ggtcacttcg gtgcgatgac cgtaatggat gccactgaac cgcgacagat tagacgatgc
     1441 ttcggcggtt gtcagtacgt aataagtggg aacaaggtaa tccagatacg tgaagtcaac
     1501 tgattctacc gtatgtccgg ctgctttcag tttttccagc aaatcatgga actgttgttt
     1561 cacttccgga tccagcttgc cattgtctat agtttgcgga taccaggcta tacggagttt
     1621 cgcgggtggt tcaagcaatg ctgcataatc gggtaccggc tggctggacg aagtagcatc
     1681 atgcggatcc tgtccggcca tgacttctgt gagcatggct gcgtctactg cattccgggt
     1741 gaaggtgccg cactgatcaa aagaagaagc gtaagctata agtccgtacc ggctgatgcg
     1801 accataggtc ggcttgaagc caacggtgcc tgtcagcgat gcgggctgac gaatagaacc
     1861 accggtatct gtgccgaggc tggccatgca cagatctgcc tgcactgcca ctgctgaacc
     1921 tcctgaagaa ccgccgggca ctttggtctc atcggcggca ttgagaacag gaccatgtat
     1981 ggaggtctca ttggatgaac ccatggcaaa ttcatcgcag ttggtgcgac cgatgataat
     2041 ggcatcttcg gccagtaatc tgtctatgca ggtagcgttg aagagacttt cgaaaccctg
     2101 cagaatagct gagctggcgg tagcttcctt gcccttcatt acgatgacat ccttcacggc
     2161 tatcaccatg ccatagagct ttcctaaagg caggtcattg cgcagttttt catccagtct
     2221 atcggcctgt atgagtgcat cttcctcaaa tacttccagc atggcattca aatgcctgtg
     2281 gtcgtgacat tggcgaacat agtgttctac cacttcacga caactcagtt cctgtttacg
     2341 cagttgttcc tgcagcacag gtaaggactc ataccgaaaa gacatgcggg cagttacttc
     2401 tcttctgact tcttgggttc ttcattcatg ccgtcacgca actctttgtt gatgttgttc
     2461 ttggcatcgt tgaattcacg gatccctttg ccgagaccct tcatgagttc cgggatcttc
     2521 ttgccgccga aaagaagtaa aatggcaaat atgataatga tccattccat tccactgggc
     2581 atgctgatga gtaacacgct gttcatctgc tgtgatttac tgcaaagata cagaattgca
     2641 gggcgctatt tggatatcca ggcagcaggg tccagcaggg tggtgttttt ccagatctcc
     2701 aggtgtactt cggtccggtc atcatctgtg taggcggtac cgatggtctg tttggtgctg
     2761 accttatcgc cggctttgac gctcacagac gtgaggccgg tatagaccgt ataatattcg
     2821 ccatgcttga tgatgacccc tttctggaag gatggattga agatgatatt gaccacttct
     2881 ccatcaaaaa ggcagcgcac tgctgctccg gcagttgtag tgatatcaat tccattattc
     2941 tctaccgtta cattcttgag tacggcgtga ggctgggttc cgaatttacc ggtgatggtg
     3001 ccacgttcta cgggccaggg taacctgccc ttgttgctgg caaagttctg gcctatgagg
     3061 gccatttccg gggtaaggcc agcgttgacc ggagcggtgg tcttcttttc ctccttgacc
     3121 gtactggtgg tggacgtacc gctttctt
//
LOCUS       DAOMAX010000296         3138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6333_length_3138_cov_3.978917, whole genome shotgun sequence.
ACCESSION   DAOMAX010000296 DAOMAX010000000
VERSION     DAOMAX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6333_length_3138_cov_3.978917"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PK742_11855"
     CDS             complement(<1..885)
                     /locus_tag="PK742_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HQU77328.1"
                     /translation="MKQLLQQRIILILLLLAVSRAFAQSTTPPFLYCATTDINGDVTL
                     LWEPSGEACGPFIEYHVFAATNLAGPYSLLATLTGVGTDTYIHVGADGAVTTWYYYIE
                     AVYDCPGYTMTPSDTLDNIDPVAPEITSVTVITGGQVRINWEPGPSPETTSYIIYRDI
                     GGFTPIDTVYGRFTTTVVDGTAAPAEQVETYTIAARDSCGNVGPFNENSHHTIKLDVD
                     WTICTDTITLLWNLYDTWPDGIEHQEIWINDDGSGAVLYDIIASFATSYVYSGPEFDD
                     GFINAFYVKAVRADGSTFS"
     gene            complement(882..2171)
                     /locus_tag="PK742_11860"
     CDS             complement(882..2171)
                     /locus_tag="PK742_11860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQU77329.1"
                     /translation="MTTKMINRTAAPDLQIPETVSLGAYRQLTLGNGVPVYIADNNTE
                     DVVKIELCFPAGRWYEPSPMLSRAVCRLLTKGTSRFSSKELADRIEFYGASVETANGQ
                     DYTSVKIFSLGKYLPEIMPLVGEMLQDASMPPEELQLFQKKQLQKLQVGLRNTDFVAN
                     RTMHAALFGDQHPYGYAVDADMIRALDTTSMRNFSRQHYQPGLATVFLSGNITDATIQ
                     ELERSVGSQTAEPITGAHQRSLHSAEEQQILVPNAESVQSSIRIASLTIGKDHPDYAP
                     LNVANTVLGGYFGSRLMSNIREDKGYTYGIYSLIRHHHNGSYFVIDTEVGNEVCRAAV
                     DEIYAEMKQLAEVPVDNNELTVVRNYMLGSLIRATDGSFNRIQVIRNMVMSGLDFEYF
                     DRLVTAVQRTRPEQIQELAGKYLDPTKQKEIICGQPI"
     gene            complement(2177..>3138)
                     /locus_tag="PK742_11865"
     CDS             complement(2177..>3138)
                     /locus_tag="PK742_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQU77330.1"
                     /translation="VNNIETALWLESDRMLHLDFSERSLDVQRNVVCEEFKEHYINRP
                     YGDVWHKLREMVYTTHPYRWPTIGLELDHIREAKLSDVESFFYSWYRPNNAILSISGG
                     ISTAKALELAEKWFGDIPAGVQPERIIPQEPAQSAARFKKVEADVPVNLVYQVWHMDG
                     RNGSGYYAQNLLSDVLGNGESSRLYQSLKMEQQLFTDISAYVSGSMDTGMFVVSGKLA
                     DGVSYEEGNAAITRELKKLQSEGCGDRELQRVKNMVETDLAGSYMNALNKAQGLAMAE
                     MLGNADLVNTEIDMYTAVTATDMDAAASLLFREDQCSTMFYGK"
BASE COUNT          721 a          811 c          771 g          835 t
ORIGIN      
        1 gctgaaagtg gagccatcag ctctgacagc tttaacataa aatgcgttga tgaagccgtc
       61 atcgaattcc ggaccgctgt acacatagga agttgcaaac gaagctatga tatcatagag
      121 tacagctccg cttccatcat cattgatcca gatctcctgg tgttcgatac catcgggcca
      181 ggtatcatac aaattccaca gcagggtgat ggtatctgta caaatggtcc agtccacatc
      241 gagcttgatg gtgtggtggc tgttctcatt aaaagggccc acattcccgc agctgtcgcg
      301 ggcagcaata gtgtaggtct ccacctgttc tgcaggagct gcagtaccgt ccaccacagt
      361 agttgtaaat ctaccatata cagtatcaat tggtgtaaag ccaccaatat cccgatagat
      421 aatatagctt gtggtttctg gcgaagggcc cggttcccaa ttaatgcgca cctggccacc
      481 ggtgatgacg gtaacagaag tgatctcagg tgccaccgga tcaatattat ccagtgtatc
      541 gctgggtgtc atggtatagc cggggcaatc atacactgct tcaatgtaat agtaccaggt
      601 ggtaacagct ccatcggcac ctacatgtat ataggtgtct gtgcccacac ctgtcagggt
      661 ggccagtaaa ctataagggc cggccaggtt cgtggcagca aaaacatggt attcgatgaa
      721 gggcccgcat gcttcgccag atggctccca gagcagggtt acatcgccgt tgatatctgt
      781 agtggcacaa tacagaaatg gcggggtagt ggactgtgca aaagcccggc ttaccgccaa
      841 taacagcagg atcaatataa ttcgttgttg taacaactgt ttcatatagg ttggccgcag
      901 atgatctctt tttgtttggt tggatcgagg tatttgccgg ccagttcctg tatttgttct
      961 ggtctggttc gttgcactgc ggtaaccagc ctgtcaaaat actcaaaatc cagtccactc
     1021 atgaccatat tacggatcac ctggatgcgg ttaaatgagc catcggtagc ccggataagg
     1081 cttcccagca tataattccg cactacggtc agttcattgt tatctaccgg aacctctgcc
     1141 agctgcttca tctctgcata tatttcgtca acggcagccc tgcacacctc attgcctact
     1201 tcagtatcta tgacaaaata actaccgttg tggtggtgtc tgatcagcga ataaatacca
     1261 taggtatagc ccttgtcttc gcggatattg ctcatgagtc tggaaccaaa gtagccaccc
     1321 aatacggtat tggccacatt taacggcgca taatcaggat ggtctttacc gatggtgagg
     1381 gaggcgatgc ggatggaaga ctgcactgat tctgcattcg gcaccaggat ttgctgttct
     1441 tcagcagaat gcagcgaacg ctgatgggct ccggtaatgg gttctgcagt ctggctgccc
     1501 acagatcttt ccagttcctg gatggtagca tctgttatat ttccagaaag gaacactgtt
     1561 gccagtcctg gctgatagtg ctgtctgctg aagttacgca tggatgttgt gtcaagcgct
     1621 ctgatcatat ctgcatctac agcgtagcca tatgggtgct gatcaccaaa cagggctgca
     1681 tgcatggtgc ggttggccac aaagtctgtg ttcctcaggc ctacctgcag cttctgcaat
     1741 tgttttttct gaaacaactg cagttcttct ggcggcatgg aggcgtcctg caacatctcg
     1801 cccaccaggg gcatgatctc cggcagatat tttcccagtg agaagatctt cacggaggta
     1861 tagtcctggc cgttcgctgt ttccacagat gcaccgtaaa attcaatgcg gtctgccagc
     1921 tctttgctgc tgaaacggct ggtacctttg gtcagcagcc gacatacggc cctgctgagc
     1981 atgggagacg gttcatacca ccgaccggcc ggaaaacaca gttcgatctt caccacatct
     2041 tccgtattgt tatcggcgat atataccggc accccgttgc ccagtgtcaa ctgcctgtag
     2101 gctcccagac ttaccgtttc aggtatctgc agatcaggtg cggctgttct gttgatcatt
     2161 ttagttgtca taacaattat ttgccgtaaa acatggtgga acactggtct tctctgaaca
     2221 acagtgatgc agctgcatcc atatcagtgg ccgttacggc ggtgtacata tctatttctg
     2281 tgttgacgag atcggcattg cccagcatct ctgccatggc aagcccctgc gccttgttca
     2341 atgcattcat ataactgccg gccagatcgg tctctaccat gttcttgacc ctttgcagct
     2401 cacgatcgcc acatccttcc gactgaagct ttttcagttc gcgggtgatg gcggcatttc
     2461 cctcttcata tgaaacacca tcggccagct tgccgctcac cacgaacatg cctgtatcca
     2521 tactgccgga cacataggcg ctgatatctg tgaacaactg ctgttccatt ttcagcgatt
     2581 ggtataacct ggatgattca ccgttgccca gcacgtcgct cagcaagttc tgggcatagt
     2641 atccggaacc atttctgcca tccatatgcc agacctgata gaccagattg accggtacat
     2701 cggcttccac ttttttgaat ctggctgcac tttgtgccgg ttcttgcggg atgatgcgtt
     2761 ccggttgtac tcctgccgga atatcgccaa accacttctc tgccagttcc agtgcctttg
     2821 cagtgctaat gcctccggag atgctgagga tggcattatt gggacggtac caggaataga
     2881 agaaggactc cacgtcactg agcttcgctt cgcggatatg atccagttcc agacctatgg
     2941 taggccagcg gtagggatgg gtggtgtaca ccatctcccg cagcttgtgc cagacatcgc
     3001 cgtatgggcg attgatataa tgctccttga attcttcgca taccacattg cgctgcacat
     3061 cgagcgaacg ctcgctgaaa tcaagatgca gcatgcggtc gctttccagc cagagtgctg
     3121 tctctatgtt attgaccg
//
LOCUS       DAOMAX010000297         3115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6412_length_3115_cov_3.808497, whole genome shotgun sequence.
ACCESSION   DAOMAX010000297 DAOMAX010000000
VERSION     DAOMAX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6412_length_3115_cov_3.808497"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PK742_11870"
     CDS             complement(<1..625)
                     /locus_tag="PK742_11870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU77331.1"
                     /translation="MDRLVSVLYFLQHRMRCIPYSQVHSPFLFSLIQEVLQDKRQYYL
                     FDRLQQERQILLTDKHLLEIEDHGAGSRTSGFQRNRSVSSIARQAVISPAQGAMLFRL
                     VRWGGYHRILELGTSLGLSAAYMAGAGSKVTVDTIEGSPAIAKEALSLWQKTGISNIS
                     LHTGTFDEQLPELLNKPSTVFDLVYIDGNHRMEPTLRYVDMITPYLSE"
     gene            740..1723
                     /locus_tag="PK742_11875"
     CDS             740..1723
                     /locus_tag="PK742_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77332.1"
                     /translation="MNKIVTLLFLSIAVTAVSAQRQSEAVPFTSDLSGLESSVRTSGD
                     TLANFALDEPCAADPIIFTSVGGGFVNGTNGYGDLEKVQLIFLPSSGTGACNEVLVFF
                     GGKSVDGDGTVKALVYEVAGDGSPGELLGESDPVNVSLCDTAGLFTAFPIATPDSFID
                     IFFAGIDVSDTYPTGDSIGVLSTDDGCGNGEEVWEKWSDGVWYPYVGEGTWNLNIALF
                     IYGVVDWEDNSPPASLDDAWAANGGLTLHPAYPDPANQQVSISYGLAEPSGVELTMYN
                     QAGQIVYQWSGQQTAGEQLIAIETSQWPSGKYYYQVAGAQGILFSSLMVQH"
     gene            1849..2619
                     /locus_tag="PK742_11880"
     CDS             1849..2619
                     /locus_tag="PK742_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQU77333.1"
                     /translation="MKKLTLSVLLLLTCFALRAQVGLRVGGHLGFNNVFIHNQNSYGY
                     SEMDYENKFGLMGGVDVAYNFDGMSGVQTEINYVKLGQDYFDRNRDFSADSDNNPKVD
                     TYRQIDLSYVQIPIFYRFQSEKRKKTVAVFHALVGPTFGFLISADMAYQADVESNGEL
                     VDIPLVIIEGAVPDFVATDAEEEPKDFFRGFDMGMQFAFGADIYANKQVYFTPQLRMF
                     YGFTDINSQPTRDLYTDYAFSRNAFVGFTVGVHYYVKQ"
     gene            complement(2670..>3115)
                     /locus_tag="PK742_11885"
     CDS             complement(2670..>3115)
                     /locus_tag="PK742_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HQU77334.1"
                     /translation="RMSRIIAEREVEKTYFVITDRCPQPENGSLTHLLWKNQKQNKSY
                     VIQGNRTGAKEARLQYELVERSESFFLLRVLLETGRHHQIRAQLAAIGCPVKGDLKYG
                     SARSNPDGSVCLHAHTLAFTHPVTGAYISICAPTPKNHPWTLFST"
BASE COUNT          764 a          689 c          776 g          886 t
ORIGIN      
        1 tttcagacag gtatggcgtg atcatatcta cataccgcaa ggtaggttcc atccggtggt
       61 tgccatcaat atataccagg tcaaaaacag tcgaaggctt gttcagcagt tcaggtaatt
      121 gctcatcaaa ggtgcctgta tgcaggctga tattggagat gccggttttt tgccacaggc
      181 tcaaggcttc ctttgcaatg gccgggcttc cttcaatggt atcaactgtg accttgcttc
      241 ctgcccctgc catgtaggca gcactgagac caagagaggt gcccagttcc aggatccgat
      301 ggtaaccgcc ccaacgcacc agccggaaca gcattgcacc ctgcgcaggc gaaatgaccg
      361 cctgccttgc tatggaagac acgcttcggt tgcgttggaa tccggaagtt ctggagccgg
      421 ctccatggtc ttctatctcc agcagatgct tgtcggttaa aagtatctgg cgttcttgct
      481 gcaagcggtc aaacagataa tactgtcgct tatcctgcag cacttcctgg atcaggctga
      541 acaggaaagg tgaatgaacc tggctgtatg gaatacacct catgcggtgt tgaaggaaat
      601 acagcacaga taccagtcgg tccatgccgc aaaagtacac cttacacgaa tgcaaacaga
      661 ggttctcctt catatgagga agaagcgttc tgaaaggaca gcggttttta gtagatttga
      721 gaacaacaaa ttgttatcga tgaataagat agttacactt ttattcttaa gtatcgccgt
      781 aacagctgtt tcagcacagc ggcaatcaga agctgttccg tttacttctg atttgtccgg
      841 tctggaatca tctgtgcgca ccagtggtga cacgctggca aactttgcgc tggacgaacc
      901 ctgcgctgca gaccctatca tctttacttc tgtaggaggg ggatttgtga acggaacgaa
      961 cggctatggt gatctggaaa aggttcagct gatctttctg cccagttccg gaacaggtgc
     1021 ctgcaatgag gtgctggtct ttttcggagg taaatcggta gatggtgatg gtacagtaaa
     1081 ggctttggtg tatgaggtgg ctggggacgg ttcacctggt gaactgttgg gtgaatccga
     1141 tcctgtcaat gtgtctctgt gtgacacggc cggtctgttt accgctttcc ctattgcgac
     1201 acctgactct ttcattgaca tattctttgc cgggatcgat gtgtcagata cctatcctac
     1261 cggcgattct ataggcgtac tttctaccga tgacggctgt ggaaacggag aagaggtctg
     1321 ggaaaaatgg tctgatggtg tctggtaccc ttatgtaggg gaaggtacct ggaatctgaa
     1381 tattgccctg ttcatatacg gggtggttga ctgggaggat aattcacctc ctgcttctct
     1441 ggatgacgcc tgggcagcta acggtggtct taccctgcat ccggcttatc ccgatcctgc
     1501 aaaccagcag gtcagtatat cttatgggtt ggctgaaccg tcgggagttg aactcaccat
     1561 gtacaaccag gctggccaga tcgtttatca gtggtcaggc cagcagactg ccggcgagca
     1621 gttaatagca attgaaacgt cacaatggcc ttctggtaag tattattatc aggttgcagg
     1681 tgcacagggt atcctgttta gcagccttat ggtgcagcat tgatcattct tgtcattctc
     1741 aaaaaggggg catttgctcc cttttttttg tcactttatt ttaacatttg ggtcaatatt
     1801 taatatagta ttacaatttg acctgtatat ttgtcacaaa aatcaaccat gaagaaactc
     1861 acattgtcgg ttctgctatt gttgacctgt tttgcactcc gtgcccaggt aggcttacga
     1921 gtaggcggac acctgggatt caataacgtt ttcattcaca atcagaacag ctacggctat
     1981 tctgaaatgg attatgagaa taaatttggc ctgatgggcg gggtggatgt tgcctacaat
     2041 tttgatggta tgagtggtgt tcagaccgag atcaactacg taaaactggg tcaggattat
     2101 tttgacagaa acagagattt ttctgctgat tctgacaata acccaaaggt ggatacctat
     2161 cgccagatcg atctgagtta cgttcagata ccgatcttct accgcttcca gtcagaaaaa
     2221 cgcaaaaaga ccgtagccgt atttcatgct ctggtaggtc ctactttcgg atttctgatc
     2281 agtgcagaca tggcctatca ggctgatgtg gaaagtaacg gtgaactggt ggatattcct
     2341 ctggtaatca tagaaggagc tgtacccgat tttgtagcta cagatgcaga agaagaacca
     2401 aaagatttct tccgcggatt tgatatggga atgcagtttg cctttggcgc agatatctat
     2461 gccaataagc aggtttattt tactcctcag cttcggatgt tctatggttt tactgacatc
     2521 aactcacagc caaccagaga tctgtacaca gactatgcgt ttagtcgcaa tgcttttgta
     2581 ggatttaccg tcggcgttca ctattatgtg aaacagtagt ctgacaaaga aaacaggaaa
     2641 gccccgtatc tgaagatttg gggctttttt tatgtgctaa acagagtcca cggatggttt
     2701 ttcggagtgg gggcacagat gctgatatag gcacctgtta ccggatgggt aaaggccagg
     2761 gtatgtgcgt gcaaacaaac tgatccatca ggattggatc tggcagagcc atatttcaga
     2821 tctcccttta ccgggcaccc aatggcagca agttgtgctc tgatctgatg gtgtcttccc
     2881 gtttctaaca atacccgcag caaaaagaaa ctttctgagc gttccaccaa ttcgtattgc
     2941 aacctggctt cctttgcgcc tgtgcgattt ccttgtatca cataactctt attctgtttt
     3001 tggttcttcc atagcagatg tgtcaatgaa ccgttttcag gttgggggca tcggtctgta
     3061 atgacaaaat aggtcttctc aacttcacgc tctgcgatga tcctgctcat tcgtt
//
LOCUS       DAOMAX010000298         3095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6474_length_3095_cov_4.482566, whole genome shotgun sequence.
ACCESSION   DAOMAX010000298 DAOMAX010000000
VERSION     DAOMAX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6474_length_3095_cov_4.482566"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PK742_11890"
     CDS             <1..1484
                     /locus_tag="PK742_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HQU77335.1"
                     /translation="RLSHPLKKLLSQTAIYGLSTIVVRMLNFLLAPLHTRVFLDQADY
                     GIISEMYAYVTFLNVIFMYGMETAFFRFASGKDPHDHRKVFGTAQGSLILTTVLFTGV
                     LLLMSSRIATFLQYPGHGEYVVYFALIIGFDSLVNIPFARLRLEQRPWRYFVIKLGSI
                     GINVFFNFFFLLPAILQKPDMFGFIGFHFKQEQAVGYVFIANLIASAATFAIFLPGMR
                     RLSVDKLLWKKLMLYGMPLVLIGLAGMINETFDRVLLKYLLPGTLEENLAQIGIYSAV
                     YKISIFMTLAVQGFRMGAEPFFFHQSSSDNAPKVYALVMKYFVIVCCIIFLGVGMFPD
                     VFRIIIGENYHEGLGIVPVLLFANLLLGVYYNQSVWYKLTDKTLYATIIPVAGAVLSI
                     VANIILIPIAGYEGAAWSRLLCYASMVILSHMIGQKYYHVPYNVRKILAYFAISIVLC
                     LAGLWLHDGLGEGSTSFALRLGLIALFTGFAWWLDGRSLLRRS"
     gene            complement(1485..2258)
                     /locus_tag="PK742_11895"
     CDS             complement(1485..2258)
                     /locus_tag="PK742_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77336.1"
                     /translation="MKRILFILAVSIALISCGSSEKNAGAQESATRFYEALKAGNVDA
                     AVLEIEPGVFDDMEPVKKALNNNLALLGNLQSYEKSSGWNINTSTESGTTVYVLFKVE
                     YAHGTSADSLTFHEGEDGQMRLIGYNWKFTQADYLDNIHASEEMATHYMEMVRDGHYD
                     MAYGMVGYQGAAATDQTKWTQMLSSTSAAAGGIQEFSIDQNQSTVNIYGQEAEVPGNY
                     YTVVINTVCANGSIRENLLFYQPDFGSDIKLVNHGRQVI"
     gene            complement(2486..>3095)
                     /locus_tag="PK742_11900"
     CDS             complement(2486..>3095)
                     /locus_tag="PK742_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQU77337.1"
                     /translation="DDATSYAGEWGVDEALQAHMAAGRSGAIIVGVDHSGPLRMMEYT
                     PWANETYGGGLGEAFSQWLMDSVVAYVDAHYRTIPDRTERYVAGSSLGGLISTYLVLA
                     HGDVFGGGAAYSPSYWFSDQLFDYAAEHTPMAPMRFEIYGGGMEDDDDMVPFMMVVYD
                     LMNQEKDPGVRLRFVVESVGQHNESWWRKQWPGTLGWLLDKQ"
BASE COUNT          680 a          825 c          754 g          836 t
ORIGIN      
        1 cgcgattgtc gcacccactt aaaaaactat tgtcgcagac agccatctat ggcctgagca
       61 ccatcgtggt gcgcatgctc aatttcctgc tggcaccgct gcacaccagg gtgttcctgg
      121 accaggccga ttatggtatc atctctgaga tgtatgccta tgtgaccttc ctcaatgtca
      181 tcttcatgta tggcatggag accgccttct ttcggtttgc ttccggaaag gatccgcatg
      241 atcaccggaa agtattcggt acggcacagg gttctctgat actgacgaca gtattgttca
      301 ccggtgtgct tttgctgatg tcatcccgca tcgccacctt cctgcagtat ccgggacacg
      361 gagagtacgt ggtgtatttt gcactcatca tcggctttga ttcactggtg aatattccct
      421 ttgcccgact cagactggaa caaagaccct ggcggtattt tgtcatcaag ctgggaagca
      481 tcggcatcaa tgtattcttc aatttcttct ttctgctacc ggccattttg cagaagcctg
      541 acatgttcgg gttcatcgga tttcatttca aacaggaaca ggcagtgggc tatgtcttca
      601 tcgccaacct catcgccagt gctgctactt ttgccatctt cctgcccggt atgcgcaggt
      661 tgtctgtgga caagctgctg tggaagaaac tcatgctgta cggtatgccg ctggtgctga
      721 tagggctggc gggcatgatc aatgagacct tcgaccgggt gctgctgaaa tacctgttgc
      781 ccggaacgct ggaagagaac ctggcgcaga tcggtatcta cagtgcggtc tataagatct
      841 ccatcttcat gaccctggcg gtgcagggat tcagaatggg agcagaacca ttcttcttcc
      901 atcagagcag ttcagacaat gcaccaaagg tctatgcgct ggtcatgaag tatttcgtga
      961 tcgtatgctg catcattttc ctgggtgtgg gcatgtttcc ggatgtgttc aggatcatca
     1021 tcggcgagaa ttaccacgaa ggtctgggca tcgttcccgt cctgttattc gccaacctgc
     1081 tgctgggggt gtattacaac cagagtgtgt ggtacaagct gaccgataag accctgtatg
     1141 ccaccatcat tccggtagca ggtgcggtgc tgagtatcgt ggccaatatc atactcattc
     1201 ccattgccgg ctatgaagga gcagcatggt ccagattgct gtgctatgcc agcatggtca
     1261 tactcagtca tatgatcgga cagaagtact accatgtacc ttacaatgtg cgcaagatcc
     1321 tggcgtattt tgccataagt atcgtcctct gtctggcagg tttatggctg cacgacggat
     1381 tgggcgaagg aagtacttcc tttgcactgc gccttggtct cattgctttg tttaccggct
     1441 ttgcctggtg gctggacggg aggtcgctgt tgcggaggtc ctgatcagat cacctgtctt
     1501 ccatgattca ccagcttgat gtcgctcccg aagtcaggct gataaaatag caggttctct
     1561 ctgatgctgc cgttggcgca taccgtattg atgaccaccg tataataatt gcccggcacc
     1621 tctgcttcct gaccatagat gttcaccgtg ctctggttct ggtcgatact gaattcctgt
     1681 atgccacccg cagctgcaga agtagatgac aacatctggg tccatttggt ctgatctgtt
     1741 gcagcagctc cctggtatcc caccataccg taggccatat catagtgacc atcacgcacc
     1801 atttccatgt agtgtgtggc catctcttca ctggcatgga tgttatccag ataatctgcc
     1861 tgggtgaatt tccagttata accgatcagc cgcatctgac catcttctcc ttcatgaaag
     1921 gtaagactgt cggcagatgt accgtgtgca tattctacct tgaacagcac atatacggtc
     1981 gtacctgatt ccgtactggt gttgatgttc cagccactgc ttttttcata ggactgcagg
     2041 ttacccagca gagccaggtt gttgttcaat gcttttttga ccggttccat atcatcaaat
     2101 acacccggtt ctatttccag cacagcagca tccacattgc ctgctttcag ggcttcgtag
     2161 aaacgggtgg cagactcctg tgcaccggca ttcttctcag aagaaccaca ggatataagg
     2221 gcgatagaaa cggctagaat gaagagtatt cttttcatgg cgttgggttg tgaaataaag
     2281 ttattggttt ttaggaactg tgccagtaag tgaatagtga accaggctaa cgcccggagt
     2341 gagtagtgag aagcatagtc ttttgatcag tgcagatctt ccaacatgtt tgtggttcta
     2401 gcttgttcgg tggctgaacc gttcaccatt cactccagac cctggtctgg ttcactgctc
     2461 accgatgccc cttcgggcat ctccttcact gcttatccag caaccaaccc aatgtgcccg
     2521 gccactgctt tctccaccag gactcattgt gctggccaac agattccacc acaaaacgca
     2581 gacgcacccc agggtctttt tcctggttca tcaggtcata caccaccatc atgaagggca
     2641 ccatatcatc atcgtcttcc atgccgcccc catatatttc aaatcgcatg ggtgccatcg
     2701 gtgtatgttc tgctgcataa tcgaagagct gatcgctgaa ccagtaggaa ggagaatagg
     2761 ccgcaccacc accgaacaca tcgccgtgtg ccaatacaag ataagtggag atgagtccgc
     2821 ccagactgct gcccgccacg tagcgttctg ttctgtcagg aatggtgcgg taatgcgcat
     2881 ctacataagc caccacagaa tccatcagcc attgtgaaaa ggcttcacct aatccgccgc
     2941 cataggtttc atttgcccag ggcgtgtatt ccatcatgcg caagggacca ctgtgatcca
     3001 cccccacgat gatggctccg cttctacctg ccgccatgtg ggcctgcagt gcttcgtcca
     3061 caccccattc tccggcatag gaggtagcat cgtca
//
LOCUS       DAOMAX010000299         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6480_length_3093_cov_4.039829, whole genome shotgun sequence.
ACCESSION   DAOMAX010000299 DAOMAX010000000
VERSION     DAOMAX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_3093_cov_4.039829"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..833
                     /locus_tag="PK742_11905"
     CDS             156..833
                     /locus_tag="PK742_11905"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HQU77338.1"
                     /translation="MPRLRISENNRFQLAVMLMIGLNIALITVRILYTGDKTFAFLGW
                     NLFLAFLPWAILVQARKLLFYRHQIPAILVTILGILFLPNAPYILTDLFHIRQNTGMP
                     LWYDTLLLASCSATGLFFFYKSLRKLEAILLYFFPGFMRIGILALVAFMSGFGIYLGR
                     YERFNSWDVFANPDMLYNSIIDPIIHPGQNAETWAMTIVYGIALLVLYWVMILLRKDP
                     VHRHYKH"
     gene            complement(830..2737)
                     /locus_tag="PK742_11910"
     CDS             complement(830..2737)
                     /locus_tag="PK742_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77339.1"
                     /translation="MKKIIGLLILLPMVFQVQAQAVRCGVSPKGVVLHYEGPMPQEKI
                     ELFRNGTAVGTIKLNKGGLSGKLEEAETFWEVYPHVQDSLVPKIEASIQKSENTETIY
                     LGYYLTVRQACGLAVVDAGGRATDTYTAKAGEREITTVWDRRDSTFMEPALSPLKYQS
                     YLSDIETGWLVNPDNRLYFARIYRRNTDSAYYRLVPDAKYRYSNLGDSLLVIAADTSL
                     PYLSYFSYIAVGYDFYTNPSARSAPLMADNLDGSTRPVLLGFTAKEENGQVDLKWKVK
                     NSSRVKSILLFRSSYSDSAFALITQLSPADTIYRDISVNPMEPVYYRLVLYDLKGLIT
                     DAPVIPMVSHEKPDVLPPAMVSISLMEDQPMIKWSECDPGARGFYVYRTDVVGREPAK
                     VSGFIPKGEEPYTWIDTTSWLKPGRSYHYSVVAESRGYVPSDYSSFVSADIPDHTPPA
                     VPVGVVARKMEDGSALIVWNTHGRQAGDPMAFFVYRSSTEEGPFIQLNEEPVFGENSF
                     TDHPGMANDTVYYTVTSLSPDGKESPQSVPVRVLLRSPDWGVRYILGKRSGDAIIISW
                     PVGEPGLAGFELYRSEDRKDPVMLTRLPAETGTFTDKDLAKGKIYAYRLVSIDANGKK
                     SQPSEWVVMEP"
     gene            complement(2747..>3093)
                     /locus_tag="PK742_11915"
     CDS             complement(2747..>3093)
                     /locus_tag="PK742_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77340.1"
                     /translation="DMNFLLNFGPTTVMMLEAAAYAKAWAGVDVLICSLCIGAMAQFI
                     VDGTLQLTPTVEADLGACASFTMFGDFCGYSMEETIGCDVEYSTSSGFDFDMQWSPCG
                     GTANKLNTSCDF"
BASE COUNT          757 a          804 c          697 g          835 t
ORIGIN      
        1 actgctgact gctgactgct gactgctgac tgctgactgc tgactgctga ctgcttccca
       61 ttgtcatttt atgttaacat tctttaatat gcgaaacaag gttgtcgctg caacgttcaa
      121 ccactataac acatccaaca agtataatac tccacatgcc tcgacttcgt atttcagaaa
      181 acaaccggtt ccagcttgcc gtgatgctga tgatcgggct caacattgcg ctgatcacgg
      241 tacgcatcct gtacaccgga gataagacct ttgctttttt aggctggaat ctttttcttg
      301 cttttcttcc atgggccatt ctggtgcaag ccagaaaact gttgttctac cgtcatcaga
      361 ttcctgccat cttagttacc atactgggta ttctttttct tcccaacgca ccatacattc
      421 ttaccgatct gtttcacatc aggcagaata ccggcatgcc gttgtggtat gacaccctgc
      481 tgctggcaag ttgctcagcc acagggctgt tcttctttta taaatctctg agaaaactgg
      541 aggccatact gctgtacttt tttccgggat tcatgcgtat tggaatattg gcactggtag
      601 cattcatgtc tggtttcggt atctacctgg gcaggtatga acgattcaat tcctgggatg
      661 tgtttgccaa tccggatatg ctgtacaact cgatcataga ccccatcatc catccgggtc
      721 agaacgccga gacctgggcc atgaccattg tttacggcat tgcgctgctg gtgttatact
      781 gggtgatgat cctgctcagg aaagatccgg tgcaccgcca ctacaagcac taaggctcca
      841 ttaccaccca ttcgctgggc tggcttttct taccgttggc atcgatcgaa acaagccggt
      901 aagcatatat cttgcctttt gccaggtctt tatctgtgaa ggtgcctgtc tctgcaggaa
      961 gtctggtaag catgacagga tctttccggt cttcgcttcg gtacagttca aaaccggcta
     1021 atccgggctc tcctaccggc cagctgatga tgatggcgtc gccgctgcgc ttgcctagta
     1081 tataacgcac accccagtcg ggagatcgca acaacaccct taccggtaca gactgcgggc
     1141 tttctttccc atcgggagaa agcgatgtaa ctgtataata tacagtatca ttggccatac
     1201 cggggtggtc agtaaagctg ttctcgccaa aaaccggttc ttcattgagc tgtatgaaag
     1261 gtccttcttc tgtagatgag cgataaacaa aaaatgccat cggatctcct gcctgacgac
     1321 catgcgtgtt ccagactatc agtgctgaac catcttccat ttttctggca acaactccta
     1381 ccggtacagc aggaggagta tgatcaggaa tatctgcgct tacgaatgat gaataatcgc
     1441 tgggaacata acctctgctt tcggcaacaa ctgaatagtg gtatgatctg ccgggtttca
     1501 gccaggaggt ggtatcgatc caggtatagg gttcttctcc ttttggaatg aaacctgaaa
     1561 cctttgcggg ctcacgacct accacatctg tacgatagac gtaaaaccct ctggcgcccg
     1621 gatcgcattc agaccacttg atcataggct ggtcttccat cagtgagatg ctaaccatag
     1681 ccggtggtaa tacatcaggt ttctcatggc tcaccatcgg tatgaccggt gcatcagtga
     1741 tcaggccttt cagatcatac agaacaagcc tgtagtaaac cggttccatt gggttgacag
     1801 aaatatcgcg gtatatggta tctgccggcg acaattgcgt gatcagcgca aaggctgaat
     1861 ctgaataact gcttctgaac aacaatatgg atttcaccct gctgctgttc tttaccttcc
     1921 acttcaggtc cacctgaccg ttctcttcct ttgccgtgaa accgagtaaa acgggtctgg
     1981 tactgccgtc cagattatct gccatcaatg gtgcagaacg ggctgacggg ttggtataga
     2041 aatcatatcc tacagcgata taactgaaat aggataagta tggcagggaa gtatctgccg
     2101 cgatcaccag cagtgaatca cccagattag aatagcggta cttagcatca ggaaccaggc
     2161 gataataggc actgtctgta tttcgtctgt aaatcctggc gaaatacaac ctgttatcgg
     2221 gattgaccag ccatcctgtt tcgatgtcac tgagataaga ctgatacttc aacggtgaca
     2281 gcgcaggctc cataaaggtt gaatctcttc ggtcccatac ggtggtgatc tcccgctcgc
     2341 cggctttggc ggtataagta tcggttgcac gtccgccggc atctacaacg gccagtccgc
     2401 aggcttgtct tactgtaaga taatacccca ggtagatcgt ttcggtattc tcagacttct
     2461 gaatggatgc ttcgatcttt ggcacaaggc tatcctgtac atgcggataa acttcccaga
     2521 aagtttcagc ttcttccagc ttaccactca gtccgccttt gttcaattta atggtaccca
     2581 cagcagttcc gttcctgaac agttcgatct tctcttgtgg cataggacct tcgtaatgca
     2641 acaccacacc tttcgggctg accccgcacc tgactgcctg ggcctgcacc tggaaaacca
     2701 tcggcaggag tatgagcagt cctataatct tcttcatgat catattttaa aaatcacagg
     2761 atgtattcag tttattggct gttccgccgc acggcgacca ctgcatatca aaatcaaacc
     2821 cggaactggt ggagtactcc acatcgcacc cgatggtctc ttccatagaa tacccgcaga
     2881 aatcgccaaa catggtaaac gaagcgcatg ctcccagatc ggcttccact gtaggagtaa
     2941 gctgtaaagt accatcaaca atgaactgtg ccatagcacc gatacacagt gagcatatca
     3001 gcacgtccac ccctgcccat gcttttgcat aagctgcagc ttccagcatc atgaccgtag
     3061 tagggccgaa gttgagcaga aaattcatat ctg
//
LOCUS       DAOMAX010000300         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6542_length_3075_cov_3.467881, whole genome shotgun sequence.
ACCESSION   DAOMAX010000300 DAOMAX010000000
VERSION     DAOMAX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6542_length_3075_cov_3.467881"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PK742_11920"
     CDS             complement(<1..511)
                     /locus_tag="PK742_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77341.1"
                     /translation="MNRSFLFTMLLGFCGLQVAKADSPVTSTDISSYYMEYEMVAKNV
                     GAFELDAATLAFLLNDQERTDVKAAVINALGWGDKVTDMGPVFRDALTAKYGTEELEA
                     YQGADLMCLAYLMAMDDYFNVAEASKIMDLAIQKSPESATVAMIRAVIKGQEAMDTNW
                     CEVWMLCSEA"
     gene            complement(504..1178)
                     /locus_tag="PK742_11925"
     CDS             complement(504..1178)
                     /locus_tag="PK742_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77342.1"
                     /translation="MGNGYERMVAKMTDEELLLHARHYQTYQEELLMAVLNEASGRGL
                     TIENKAAIESLIRQYEDEARHQAEVAELEKGREDDQQVEETEDPDSSKSLPVLYSQTA
                     VLAFSLFFSPLAGSILLSINIRKLQRKGAGQVLVFGLVLAIAQGIINMQVAPDSIISI
                     LAHVAGALLLSELMWNQFIGRRIPYTRRNILIPLLIAIAIIAPLAWYLYQHPELMEQL
                     QNGTYE"
     gene            1246..2040
                     /locus_tag="PK742_11930"
     CDS             1246..2040
                     /locus_tag="PK742_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77343.1"
                     /translation="MTALVYAQNESGLSGRFILIDSLQTPSGITFGPAQYARFADALI
                     CGVTIDQADTVRTGIFLLPLNEPNAVSTELKIPSMGSNTSYLQGSSSADGSILVFTVN
                     HYNGWTGNDLAMCTRLDDGSYSYPALLSGINKKDTADCYPWIADDGRRLYYLQDERLM
                     VAEWNASEKSFTTATPVLYTGTVELNILSVWLDSKEKHMWLVSDNKIYRASRKNRKKP
                     FSMPELYTDEFADWSFISGISFTPDEQDMYLYYADEFSNLLHFKPR"
     gene            2040..2624
                     /locus_tag="PK742_11935"
     CDS             2040..2624
                     /locus_tag="PK742_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77344.1"
                     /translation="MTGKGLSWRDRIFWACVGIISIVSVYFVSRYLISGNSEYAGFDK
                     HTFHDQYEIYTPPYLLPDDSLNSAAILRFSDFSNEVFIMVLEEKKRDLDRRGIHPVLA
                     EYFLYIQQNLMEKLDSPVIVSQNDHFLHGLHAIQCELEGMYDSIPVYYIFSTYETEQS
                     FLQVRGWTETALKPAVKQDLFASIRSFAPVVKDK"
     gene            2724..>3075
                     /locus_tag="PK742_11940"
     CDS             2724..>3075
                     /locus_tag="PK742_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77345.1"
                     /translation="MNSFHGYLRYSVEATSEDSTDVATFYMFSPRYVELWMNDGFLRM
                     IETGGASRGNILIDRQQQLAYQIDTVHQQVYRGVYSDFEEASDELQAMMPDHYRPALE
                     NLQNDTTIAGLHCTE"
BASE COUNT          787 a          752 c          697 g          839 t
ORIGIN      
        1 atgcttcact gcaaagcatc cagacctcac accagttggt gtccatagct tcctgacctt
       61 tgataacagc acggatcatg gctactgtag cactttcggg agatttctgt atggccagat
      121 ccatgatctt agaagcttcc gctacgttaa agtaatcatc cattgccatc agataggcca
      181 ggcacatcag atctgctccc tggtatgctt ccagctcttc tgtcccgtat ttggcggtaa
      241 gtgcatcacg gaaaaccggc cccatatcag taaccttgtc tccccacccc agtgcattga
      301 tcactgccgc cttgacatcg gtcctttcct gatcattaag taaaaatgca agggtggctg
      361 catccagttc aaatgcaccc acattcttgg caaccatctc gtattccatg taataggaag
      421 aaatgtctgt acttgtaacc ggcgaatctg ccttggcaac ctgcaggccg caaaatccca
      481 gcaacatggt aaaaaggaaa gatctattca tatgtgccat tttgaagttg ttccatcagt
      541 tcgggatgct ggtacaggta ccaggccagc ggagcgatga tcgctatagc tattagtaag
      601 gggatcagga tgtttcttct ggtgtaaggt atcctacggc ctataaactg gttccacata
      661 agctctgaga gcaagagggc tccggccaca tgggccaaaa tgctgatgat gctgtcggga
      721 gctacctgca tattgataat gccctgggca atagccagta ccaagccgaa cacgagcacc
      781 tgccctgctc ctttgcgttg aagttttctg atattgattg ataacagaat actgccagcc
      841 aacggagaaa aaaacaggct gaatgccaaa acagctgtct gcgaatagag tacaggaagc
      901 gatttgctgc tatccgggtc ttctgtttcc tccacctgtt gatcgtcttc cctgcccttt
      961 tccagttcag ccacctctgc ctgatgcctg gcttcatcct catactgcct gatcagcgat
     1021 tctatggcag ctttattctc tatggtcagc cctctgccag acgcttcgtt cagcacggcc
     1081 atcagcaatt cttcctgata agtctgataa tgacgggcat gcagcaacag ctcttcgtca
     1141 gtcatctttg ccaccatgcg ttcatacccg tttcccatat agctttctaa attctatttt
     1201 tgacagatgc gtgtaaaaat actattattc aacctatgcc tttggatgac cgccttagtt
     1261 tatgcccaga atgaatccgg gctaagcggc aggtttattc tgatagattc cttgcaaacc
     1321 ccttcgggca taacttttgg ccctgcccag tatgccaggt ttgctgacgc cctgatctgt
     1381 ggcgtaacta tagaccaggc agacacggtc agaaccggca tattcctgtt gccactgaac
     1441 gaacctaatg ctgtatcaac tgagttgaag ataccgtcta tgggttccaa tacatcctat
     1501 ctgcagggct cttcatctgc agacggcagc atactggtat ttactgtgaa tcactataat
     1561 ggctggaccg ggaatgatct ggctatgtgc acccgcctgg atgatggttc ttattcctat
     1621 cctgccctgc tttccggaat caacaaaaaa gatacggccg attgctaccc gtggatagct
     1681 gatgatggcc gccgtttgta ttacctgcag gatgaacgac tcatggtggc agagtggaat
     1741 gcctctgaaa aatcctttac aaccgctact ccggtgctat acacaggaac agtagaactg
     1801 aatatcctga gtgtctggct ggacagcaaa gaaaaacata tgtggctggt ttctgataat
     1861 aagatctaca gggcttccag aaaaaacaga aagaaacctt tttcgatgcc cgagctttac
     1921 actgatgagt ttgctgattg gagttttatt tcaggtatct catttacccc tgatgaacag
     1981 gacatgtacc tctattatgc cgatgaattc agtaaccttc tgcatttcaa acctcgctga
     2041 tgaccggaaa agggctgtct tggagagacc ggatattctg ggcttgtgta ggaatcattt
     2101 ccatagtatc cgtgtacttt gtgagccgct acctgatctc agggaattct gaatatgccg
     2161 gtttcgacaa gcatactttt catgaccagt atgaaatata taccccacct tatctgctgc
     2221 cggatgacag cctgaatagt gcagcaatac tgcgattcag tgatttcagc aacgaagtat
     2281 ttatcatggt gctggaagaa aaaaaacgag atcttgacag acggggaatt catccggtcc
     2341 tggcagaata tttcctgtat atccagcaga atctgatgga gaaactggat tctcctgtca
     2401 ttgtaagtca gaatgatcat ttcctgcacg gtttgcacgc aatccaatgc gaactggagg
     2461 gcatgtatga cagcattcct gtttattata tcttttctac atacgagacc gaacagagtt
     2521 ttcttcaggt aagagggtgg accgaaacag cactcaagcc tgcggttaaa caagacctgt
     2581 tcgcttctat tcgcagcttt gctcccgttg ttaaggataa ataggcctac ctttggaggg
     2641 tgagatctgt atcctttttg tccatggtgc tcctgcttgt ttgggcaggt tgcggccact
     2701 catctgaaca gcaagcacgc aacatgaata gctttcatgg ctacttgcgc tattccgtag
     2761 aagcaacttc tgaagacagc acggatgtag ccacttttta tatgttcagc cctcgatatg
     2821 tggaactctg gatgaacgat ggttttttgc gcatgatcga gaccggaggc gcaagccggg
     2881 gtaacattct catagacaga caacaacaac tggcttacca gatcgataca gtgcatcagc
     2941 aggtgtatcg aggcgtgtac agtgactttg aagaagcctc agatgaacta caggctatga
     3001 tgcctgatca ttaccgcccg gctctggaaa atcttcagaa tgataccacc attgcagggc
     3061 tgcactgtac tgaat
//
LOCUS       DAOMAX010000301         3053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6636_length_3053_cov_4.849566, whole genome shotgun sequence.
ACCESSION   DAOMAX010000301 DAOMAX010000000
VERSION     DAOMAX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6636_length_3053_cov_4.849566"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PK742_11945"
     CDS             <1..270
                     /locus_tag="PK742_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQU77346.1"
                     /translation="DIDLLAGVLSGTILPVNGKSTFKDEFVTAGGVDTRDIQFTTMEH
                     KQFPGLHFAGEVLNIDAVTGGFNFQAAWTTAYIAAEAMAGKLTQV"
     gene            complement(319..1185)
                     /locus_tag="PK742_11950"
     CDS             complement(319..1185)
                     /locus_tag="PK742_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQU77347.1"
                     /translation="MKKIIIIVSVFIVLNSCNEKSPVNEFVPEDEETEQAAIAQATKH
                     APQVMECFGYIDVPPSSMAEIHARSSGYISGIAVLEGTQVSKGTLLAEISSPDFALLQ
                     RQLQEAGANWSFEQARFARMSGLYEKNALSAADWETAQRDHALAKATFSGLKEQLDAI
                     GFDTSRVLAGQIQTALKLYSPIHGVVTEVHASNGQMATPGDHLFSVIDKSHLHVELQI
                     PASDVQQIELEDQFRFTPAGTLDTLFGEVHLINDVVDEATNTVRVHGHLLDDAHADHL
                     LVGERVFVVFEE"
     gene            complement(1182..>3053)
                     /locus_tag="PK742_11955"
     CDS             complement(1182..>3053)
                     /locus_tag="PK742_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQU77348.1"
                     /translation="LSPMQISREDARRLIKVGVNVRDVDVATLVADIENALRPLELPP
                     GYYIAYGGAFENLQHAVERLSVAVPVALLLILFLLYITFNSVSKALIIFVAVPLSAVG
                     GVLALQLRGLPFSISAGIGFIALFGVAVLNGIVLMNEFIRLSKKEDMSLKDIVITAGV
                     SRLRPVLMTAMVASLGFMPMALSTGSGAEVQRPLATVVIGGLITATFLTLVVLPALYY
                     LYEHTRLRLRPAAVSMLSGLLFIPFAGNAQTYNEQELIMKAAQHWPALAQADNRIAHW
                     EAEKRRSWILPPATVDIQYGQINEVVNDLQWGVDQQLGKPWSAGVNRAFAEAGAAQAV
                     AIRELTMASLAYQVRTALAGYQQFRAEQAFLDSVSDMTSTALALVNTRREAGMLTLAE
                     QVMMEQVIRDLLTMRYTSGTKLTESLGMLQYLCGDSTLIPDPDLKVRDDHMIPGELRP
                     SSLQIRPSELAVEMSVRQMDVIRSHRIPDLSLGYFRQSLGGVNGFQGVRAGISIPILH
                     SGTSVMLEQQELQQQNLQWQVQQDQHQLEMQIRQLSETRMLTKELLSSWPEDPLAMYS
                     SSIAELQNRFYDGQIDVYQYCQQLTVQLEAAIAWIGLISSYNMQTIQLSFLTENDIK"
BASE COUNT          794 a          811 c          708 g          740 t
ORIGIN      
        1 gatatagacc tgctggccgg tgtgttaagc ggaacgatct tacctgtcaa tggcaagagc
       61 actttcaaag atgaatttgt cacagcaggc ggagtggata cccgcgatat tcagttcacc
      121 accatggaac acaaacagtt tcccggtctg cactttgccg gtgaagtgct gaacatcgat
      181 gcggtgaccg gtggttttaa ttttcaggca gcctggacca cagcttatat agcggcagaa
      241 gcgatggccg gcaaactaac gcaggtctga cctgtatttc gttccctaca ggagagtcag
      301 tcaacagctg tgacctgatc actcctcaaa aacaacaaaa acacgttcgc ccaccagcag
      361 gtgatctgca tgcgcatcat cgagcagatg tccgtgtacc ctgacagtgt tggttgcctc
      421 atccacaaca tcattgatca aatggacctc tccgaacagt gtatcaaggg tacccgctgg
      481 tgtaaaccgg aactggtctt ccaattcaat ctgctgaaca tcagaagcag ggatctgcaa
      541 ctccacgtgt aaatgcgatt tatcaatgac cgaaaagaga tgatcacccg gggtggccat
      601 ctgcccgttg gaagcgtgta cctccgtcac tactccatga ataggtgagt agagtttcaa
      661 agcagtctgg atctgaccag ccagtacacg acttgtgtca aatccgatgg catcaagttg
      721 ttccttcaat ccactgaaag tggcctttgc caatgcatgg tcacgctgtg cagtttccca
      781 gtcagcagct gaaagggcat tcttttcata cagcccgctc attctggcga atcttgcctg
      841 ttcaaagctc cagttggctc cagcctcctg caattgccgt tgcaataagg caaaatcagg
      901 tgatgatatc tcagccagca atgtgccctt actgacttgt gtaccttcca ggacggcgat
      961 accggaaata tacccggatg atcttgcgtg tatctctgcc atactggagg gaggaacatc
     1021 gatataacca aaacattcca ttacctgtgg tgcatgtttc gtcgcttgag caattgcagc
     1081 ttgttcagtt tcttcatcct ccggtacaaa ctcatttacc ggtgactttt cattgcatga
     1141 attcagcaca ataaaaacag aaactattat tattattttt ttcattttat atcattttca
     1201 gtaagaaaac tcagttgaat ggtttgcata ttgtaggagc tgatcaggcc gatccatgct
     1261 atagccgctt cgagctgtac agtaagctgc tgacaatact gatagacatc aatctgtcca
     1321 tcgtagaatc tattctgcag ttcagcgatg gaagagctgt acatggcaag cggatcctcg
     1381 ggccatgacg agagcagctc ttttgtgagc atcctcgttt cagataattg cctgatctgc
     1441 atctccagct ggtgctgatc ctgttgcacc tgccactgca gattttgttg ctgcagctcc
     1501 tgctgctcta acatgaccga tgtgccactg tgcaaaatag gtatggaaat tccggctcgc
     1561 accccctgga atccattcac gcctcccaga gactgtctga aataaccaag actcaggtcc
     1621 ggtatccggt ggcttctgat cacatccatt tgtcggacag acatctctac agccagttcg
     1681 gatggtctta tctgaagaga tgaaggccgc aattcgccgg gtatcatatg gtcgtctctt
     1741 accttcagat ccggatctgg aataagcgtg ctgtctccac acaggtactg aagcattcca
     1801 agactttcag tgagctttgt acctgaagta tagcgcatgg tgagtaaatc acgaatcacc
     1861 tgttccatca tcacctgctc ggccagcgta agcatacccg cctctcttcg ggtattgacc
     1921 agggcaagtg cggtagatgt catatcggaa acggaatcca gaaacgcctg ttcagccctg
     1981 aactgctgat aaccggcaag tgctgttcgc acctgataag ccagtgaagc catagtcagc
     2041 tcccggatgg caacagcctg cgctgcgccg gcctcagcaa atgccctgtt gacccccgct
     2101 gaccagggtt tacccaactg ctggtcaaca ccccactgaa gatcattgac cacctcattg
     2161 atctgtccgt attgaatatc aacagtagcc ggaggcaata tccatgagcg ccgcttctca
     2221 gcttcccagt gggctatgcg attatcagcc tgtgccaatg caggccagtg ctgagctgct
     2281 ttcatgatca gttcctgttc attgtatgtt tgggcattgc cggcaaaagg aatgaacagc
     2341 aggccggaca gcatggacac cgcagcaggg cgcagccgca aacgggtatg ctcatacaaa
     2401 taatacaagg caggtaagac caccagtgtc agaaaggtag cggtgatcag tcccccgatc
     2461 actacggtgg ccagaggacg ttgcacctca gcaccacttc ccgttgacaa agccatcggc
     2521 ataaaaccaa gagaggccac catggctgtc atcaggaccg gccgcaatct ggaaactcct
     2581 gctgtgatca caatgtcttt caaactcata tcttcttttt tactgagtct tatgaattca
     2641 ttcatcagta cgatcccatt cagaacagct acaccgaaca gagcaataaa accgatccct
     2701 gccgaaatac tgaatggcag tcccctcagc tgaagggcca gcacaccacc aactgcagat
     2761 aaaggcaccg caacaaaaat gatcagcgcc ttactgacag aattaaaagt gatgtagagc
     2821 aggaacagga tcagcaacag tgcaacggga acggctacag acaagcgttc aacagcatgc
     2881 tgaaggttct cgaatgcgcc gccatatgcg atgtaatatc cgggaggcag ctccagtgga
     2941 cgcagggcat tttctatatc ggccaccagg gttgctacat ctacatcccg cacattcacc
     3001 ccaaccttga tcagtcttcg ggcatcttca cggctgatct gcataggact tag
//
LOCUS       DAOMAX010000302         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6663_length_3045_cov_4.835452, whole genome shotgun sequence.
ACCESSION   DAOMAX010000302 DAOMAX010000000
VERSION     DAOMAX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6663_length_3045_cov_4.835452"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="PK742_11960"
     CDS             <1..1854
                     /locus_tag="PK742_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77349.1"
                     /translation="FRSARLDPQYRKYYGNGILKAKDALAITPASVVQHIKLSPEASA
                     NRSFVGNLISMYFRAGNKQVQEDLKEMMEMELVQLCHRIPALHQLLLKEQDDVITDQE
                     KAEVLEVLRSSDLASDFLKANLAGNSGANRSLISLGEVSNVEWLLDGHRYFLRSSGVQ
                     YRVKKEPFISYTEADSQTRIDTMEIEVGAVTRSGSTELVLLDNQDELGRQTVVLMETE
                     VDGRTTYEWLFRDEGSADDKKDRSLTASVMPEGLNIPLHTSATGFRGNGKVLKAVLHV
                     ISWASAGKKKKEWRKWLSSTHTDKYGIMLYDLEQDDPSGRAWVNSTLADQSVWKTIQD
                     DQRPVLCLVPGTFREVQENYDEFLSNAAVRSALRQKSCRYVIGLNHPSVVDGIAHNAG
                     ELDKLLKGKLKKKECTVIAKSRGGLVARYLFEKEWVTASGAPSANAPLRLRNLVMLGT
                     PNQGTHMASNTKWKNMINVITNVAGKAFSLGSPLFKGLQVLASAVVNEITDLPGLDDM
                     EIKGDFLRNMNKQLGVKNNYFIVTANYEPDPLLRKFFDNVFIDQVIFERMENDAVVPV
                     RSVFFAMSSVQDVVPDSNRRIFGSDPSLNHFAYTRPEHPELVNWIMQQAFS"
     gene            1851..2258
                     /locus_tag="PK742_11965"
     CDS             1851..2258
                     /locus_tag="PK742_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77350.1"
                     /translation="MTDRLDIGVAYDIEVPANEYLSGSIRPTVLAGESYCITVPPEQR
                     WKDWFISCGPEGHGLRFGRKKLRLPGQRYFCLCGGVETASGTPQDLFGIGQAEGLIIQ
                     IPIAGRLYFFANDWPDKYGNNHGSVRLSIIRLS"
     gene            complement(2464..>3045)
                     /locus_tag="PK742_11970"
     CDS             complement(2464..>3045)
                     /locus_tag="PK742_11970"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HQU77351.1"
                     /translation="FEHLINAYLEDQVGTMSGFLSPELTAGLRRNLLQKKEEGMMHPA
                     GIGKHFDFQRNLEIRGDVICWIEEDTDDPFEQEFLDQMRRFVEHLNRTCYTGIRSFEF
                     HYALYEAGSFYKRHKDQFQQDRGRQFSVVHYLNEQWQPEDGGQLKVWTRHGEQLIQPE
                     GGRVVFFKADQTEHEVLPASRARMSIAGWLKNV"
BASE COUNT          799 a          733 c          815 g          698 t
ORIGIN      
        1 ttccgttcag caagacttga cccgcaatac agaaagtatt atggtaatgg tattctgaaa
       61 gcgaaagatg cactggccat tacacctgca tccgtggtac agcatatcaa actgtcgccg
      121 gaggccagcg ccaacagatc ctttgtgggc aatctgatct ccatgtattt cagagcagga
      181 aacaagcagg tgcaggaaga cctgaaagag atgatggaaa tggaactcgt gcaactgtgc
      241 catcgcatcc cggcgctcca ccagctgctg ctgaaagaac aggatgatgt gatcacggat
      301 caggaaaaag cggaagtgct ggaagtgctg cgcagctcag atctggccag tgatttcctg
      361 aaggcaaatc tggcaggtaa cagcggtgcg aacagatcac tgatcagttt aggcgaagtg
      421 tctaacgtag aatggttgct ggacggacac agatattttc tgcgttcttc cggtgtacag
      481 taccgggtga aaaaagaacc attcatcagc tatacggagg cagattcgca aaccaggatc
      541 gacaccatgg agatcgaggt cggtgccgtt accagaagtg gcagcacaga actggtgctg
      601 ctggataacc aggatgaact gggcagacaa acggtcgtgc tcatggaaac ggaggtggat
      661 ggcagaacta cctatgaatg gttgttccgc gatgaaggat ctgctgatga caaaaaagat
      721 cgctccctca ccgcatccgt gatgcccgaa ggactgaaca ttcccttgca tacctcggca
      781 accggttttc gcggtaacgg gaaagtattg aaagctgtgc tccatgtgat cagctgggca
      841 agcgcaggca agaagaagaa ggaatggagg aaatggctga gcagtacgca tacagacaag
      901 tatggcatca tgctctatga cctggaacag gatgacccgt ccggcagagc ctgggtcaac
      961 agcactctgg ccgatcagtc cgtatggaag accatccagg atgatcagcg tccggtcttg
     1021 tgtctggtgc ccggcacctt ccgcgaggta caggagaact atgatgagtt cttatccaac
     1081 gctgctgtgc gatcagcact cagacaaaaa agctgccgct atgtgatagg cctgaaccat
     1141 ccgagcgtgg tggatggcat tgcgcacaat gcgggtgaac tggacaagct gctgaaaggc
     1201 aaactgaaaa agaaggaatg tacggtcata gccaaaagcc gtggcggact cgttgccagg
     1261 tatctgttcg aaaaagaatg ggtaacggca tccggagcac cctccgcaaa tgctccgctc
     1321 agactgcgca acctggtcat gctcggaacg cctaaccagg gcacacatat ggcaagtaat
     1381 accaagtgga aaaacatgat caatgtgatc accaatgtgg ctggtaaggc attcagcctg
     1441 ggaagtccgc tgttcaaggg cttgcaggta cttgcctcgg ctgttgtgaa tgaaattact
     1501 gatctgcctg gcctggatga tatggagatc aaaggagatt ttctccgcaa tatgaataaa
     1561 cagctgggcg tgaagaacaa ctactttatt gttacagcca actatgaacc ggatccgtta
     1621 ctgagaaagt tcttcgataa tgtgtttatt gaccaggtca tatttgaacg catggagaat
     1681 gatgcggtcg ttcctgtcag gtctgtcttt tttgccatgt cttctgtgca ggatgtggtg
     1741 cctgattcga acagaaggat attcgggtct gatccgagtc tcaatcattt tgcatacacc
     1801 agaccggagc acccggagct ggtcaactgg atcatgcagc aggcattctc atgacagaca
     1861 gactggacat tggcgtggca tacgacatag aagttccggc gaacgaatac ctgagcggat
     1921 cgatcaggcc cacggttctg gcaggcgaat catattgtat aacagtccct ccggagcagc
     1981 gatggaaaga ctggttcatc tcctgcggac cggaagggca tgggctgcga tttggcagaa
     2041 aaaaactgcg attgcccggg caacgatatt tctgtctttg cggcggtgtc gaaacggcgt
     2101 caggaacacc acaggatctg tttggaatag gacaggcaga agggttgatc attcagatac
     2161 ccattgctgg caggctctat ttctttgcca atgactggcc agataaatac ggaaacaatc
     2221 atggttctgt caggctgtcg atcattcgac tgagctgaga ttcctatggc tgtaggaagt
     2281 acattcgcat aaggcatgct aaacggtaac cggagcctca ggccacatcg ggaatgtggt
     2341 atgccgccag ttgcggccaa aggggcatcg ggtgaaagcg ctgtatcacc acaagttgtt
     2401 ctgttaaaaa ttccgggatt atccggccga tagaaaagcc gttttcgcta tctcctgtga
     2461 ggatcagaca ttcttcagcc agcccgcaat gctcatgcgt gctctggaag ccggtaacac
     2521 ttcgtgttcg gtctgatcag ctttgaaaaa gaccacacga ccgccttccg gttgtatgag
     2581 ctgctccccg tgacgagtcc agaccttgag ttgtccgcca tcttccggct gccattgctc
     2641 attcagataa tgtaccactg agaattgcct accccggtcc tgctggaact ggtctttatg
     2701 acgcttgtaa aaactgcccg cttcatataa ggcataatgg aattcaaaac tcctgattcc
     2761 ggtatagcag gtccggttga gatgctccac gaatctacgc atctgatcca ggaattcctg
     2821 ttcgaacgga tcatcagtat cctcctctat ccagcagatc acatcacccc tgatctccag
     2881 attgcgctga aaatcgaaat gttttccaat gcctgcagga tgcatcatcc cttcctcttt
     2941 tttctgcaag aggttgcgcc gcaatccggc tgtcagctcc ggcgacaaaa agcctgacat
     3001 ggttccgacc tggtcttcca ggtaagcatt gatgaggtgt tcgaa
//
LOCUS       DAOMAX010000303         3042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6675_length_3042_cov_5.054235, whole genome shotgun sequence.
ACCESSION   DAOMAX010000303 DAOMAX010000000
VERSION     DAOMAX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_3042_cov_5.054235"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..455)
                     /locus_tag="PK742_11975"
     CDS             complement(141..455)
                     /locus_tag="PK742_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQU77352.1"
                     /translation="MKSFRQRVRSWKIPRILLNRYVFTLFVFAIWMTFFDQNNLFVQL
                     DRYRNLQDARQKIDFYKQETANTQAQLYDLMTNETTLEKFAREKYYMKKPNEDVFVIV
                     EE"
     gene            complement(537..1817)
                     /gene="eno"
                     /locus_tag="PK742_11980"
     CDS             complement(537..1817)
                     /gene="eno"
                     /locus_tag="PK742_11980"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQU77353.1"
                     /translation="MSNIIDISARQILDSRGNPTVEVDVFTEYGAAGRAAVPSGASTG
                     THEAVELRDGDDSRWMGKGVEQAVENVLTILAPELLGVSVLDQNYIDDLMLEVDGTPN
                     KARVGANAILAVSMACAKAAAHELGLPLYRYIGGTNANTLPVPLMNILNGGAHADNKI
                     DFQEFMIAPVCGGSFSEALRAGVEVFHHLKKILKKNGYSTNVGDEGGYAPDIQSNEEA
                     IETVLMAIEAAGYRPGEDIYIAMDAATSEFYNAETGLYTFKKSSGKQMNSEEMVQYWS
                     EWCNRYPIVSIEDGLAEDDWSGWKKLTSAIGSKVQLVGDDLFVTNVTRLQKGIDENIA
                     NSILVKVNQIGSLTETINAVNLAHRNRYTSVMSHRSGETEDTTIADLAVALNCGQIKT
                     GSASRSDRIAKYNQLIRIEEELGSSAVWNGNIFG"
     gene            complement(1953..3041)
                     /gene="carA"
                     /locus_tag="PK742_11985"
     CDS             complement(1953..3041)
                     /gene="carA"
                     /locus_tag="PK742_11985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQU77354.1"
                     /translation="MEKVPAVLLLEDGTAYHGFSAAKIGTTTGEICFNTGMTGYQEIF
                     TDPSYAGQILLMTNAHIGNYGAFREETESKDIKISGLVVKNYTVQYSRRMTEQSIQDY
                     LLDENLVGIYGVDTRAIVRHIRDKGAMNAIISSETTDLEELKRQLDAVPSMAGLELAS
                     TVSTKESYFVGDPNAGRKVIVYDFGVKKSILSNLTDRDCYLKVLPAKTPFSEAIEWNP
                     DAFFISNGPGDPASMDYAVQTVKEILNSGKPLFGICLGHQLLALANGIGTYKMHNGHR
                     GANQPVKNLVTGKSEITTQNHGFGVNPDDVRNSPNTIITHVNLNDDTIEGIRLKDKPA
                     FSVQYHPEASPGPHDARYLFDEFVGMMK"
BASE COUNT          661 a          773 c          763 g          845 t
ORIGIN      
        1 ccatgattca cttcattgcc aaatctcatt tatggtctca aagaatccct gaagggtgac
       61 tctgtgggaa cgctaaatat ctttccggaa gcacacctga gaagtaactg caaactatag
      121 atcgccaatt gccacttgat ctactcttcc acgatcacaa agacatcttc attcggtttc
      181 ttcatgtagt acttctcacg tgcaaatttc tcaagcgtgg tttcattggt catgagatca
      241 tacaactgag cctgcgtatt tgcagtttcc tgtttgtaga agtctatttt ctgtctggca
      301 tcctgcagat ttctgtagcg atccagctgt acaaagagat tattctggtc aaagaaggtc
      361 atccagatgg caaagacgaa cagggtaaag acatacctgt tcagcagaat gcgtgggatc
      421 ttccaggagc ggaccctttg acgaaatgat ttcacggtat gaaaataaaa agccatcgtc
      481 catttggaac gatggctttg caatggtaat tgtgtattga cgtatcagct ggacactcaa
      541 ccgaagatat tgccattcca tacagcagaa ctgcccagtt cttcttcgat gcggatgagc
      601 tggttgtact tggctatccg gtcactgcga ctggcagaac ctgtcttgat ctgtccgcaa
      661 ttcagggcca ctgccaggtc ggcgatggtg gtatcttctg tttcgccgga acggtggctc
      721 attacactgg tgtagcggtt gcgatgtgcc agattcacgg cattgatggt ctccgtcagc
      781 gaaccgatct gattcacctt caccagaata ctgttggcaa tgttttcatc aatacctttt
      841 tgcaggcggg tcacattggt aacgaagagg tcatcgccca ccagctgtac cttactgccg
      901 atggctgagg tcagtttctt ccatccgctc cagtcgtcct ctgccagacc atcctcgatg
      961 gaaacgatcg gatatcggtt acaccattca ctccagtact gtaccatctc ttcagagttc
     1021 atctgtttgc cggatgattt cttgaaggtg tacaaaccgg tctccgcatt gtagaattca
     1081 gaggtggcag catccatggc tatgtagatg tcttcgccgg gacgataccc tgcagcttct
     1141 atcgccatca gtacggtctc aatagcttct tcgttgctct gaatatccgg agcatatccg
     1201 ccttcatccc ccacattggt ggaatagccg ttcttcttga ggatcttttt caggtgatgg
     1261 aagacttcca cgccggcacg cagcgcttca gaaaaagagc cgccgcaaac cggtgcgatc
     1321 atgaattcct ggaagtcgat cttgttatct gcatgcgcac cgccgttgag gatgttcatc
     1381 agcggcacag gcagggtgtt ggcattcgta ccgccaatat agcgataaag cggaagcccc
     1441 agttcatggg cagctgcttt ggcgcaggcc atactcaccg ccagaatggc attggcaccc
     1501 acacgtgctt tgttgggtgt gccatctact tcgagcatca ggtcgtcaat atagttctgg
     1561 tccagtacgc ttactcccag cagttcaggg gccaggatgg tcagcacatt ttctaccgcc
     1621 tgttccacac cttttcccat ccagcgggaa tcgtcgccgt cacggagttc aaccgcttcg
     1681 tgtgtgcctg tagatgcacc ggaaggaaca gcggcacgtc cggcagctcc gtattcggtg
     1741 aatacatcta cttccacagt agggttacct ctggagtcga ggatctggcg tgcggagatg
     1801 tcaatgatat tgctcatggt gggttataag ttgttggtta ttagtaattg gtttttagtt
     1861 cggttttatc ggaaacctcg ttatagttat cctgctctat tttttcagtg ttagtttgct
     1921 ttccaggatc tctcactact caatacccac tactacttca tcattcctac aaactcatca
     1981 aacagatacc tcgcatcatg cggaccgggg cttgcctccg gatggtactg tacgctgaag
     2041 gcaggtttgt ctttcaggcg gatgccttct atggtgtcgt cgttcaggtt gacgtgggtg
     2101 atgatggtat tgggcgaatt gcgcacatcg tcagggttca caccaaaacc gtggttctgg
     2161 gtggtgatct cacttttgcc ggtcaccagg tttttgaccg gttggttggc tccgcgatgg
     2221 ccgttatgca ttttataagt gccgatgcca ttggccaatg ccagcagctg atgccccaga
     2281 caaatgccga agagtggttt gccgctgttc aggatctcct tcacggtctg caccgcatag
     2341 tccatggaag ccggatcgcc gggaccgttg gaaatgaaga aggcgtccgg gttccattct
     2401 atggcttcac tgaagggtgt tttggcagga agtactttta ggtagcaatc gcggtctgtc
     2461 agattggaca ggatactttt cttgacaccg aagtcataga cgatgacctt tctgcctgca
     2521 ttcggatcac ccacaaaata ggactctttg gtgctgaccg tactggccag ttccagtcct
     2581 gccatagagg gtactgcatc aagctgacgc ttcagttctt ccaggtctgt ggtctcgctg
     2641 gagatgatgg cattcatggc gcctttgtcg cggatatgcc tgacaatggc acgtgtatcc
     2701 acgccgtaaa tacccaccag gttctcatcc agcagataat cctggatgga ctgttcggtc
     2761 atccgtctgc tgtactgcac cgtgtagttc ttgaccacca gcccggatat cttgatgtcc
     2821 ttgctctcgg tctcctcacg gaaggcaccg tagttaccga tatgcgcatt ggtcatcagc
     2881 aggatctggc ctgcatagct ggggtcagta aaaatttcct ggtaaccggt catgccggtg
     2941 ttgaagcaga tctcgccggt ggtggtaccg atcttggcag cgctgaaccc atggtatgcg
     3001 gttccatcct cgaggaggag tactgccggc accttttcta ta
//
LOCUS       DAOMAX010000304         3028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6732_length_3028_cov_3.678776, whole genome shotgun sequence.
ACCESSION   DAOMAX010000304 DAOMAX010000000
VERSION     DAOMAX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6732_length_3028_cov_3.678776"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="PK742_11990"
     CDS             complement(<1..1839)
                     /locus_tag="PK742_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU77355.1"
                     /translation="MKTTGTLIILLLAGMLKAQTFQISGHVSDELKLPIPFATILVEN
                     GQGTAADESGHFYLELPPGEHFFQISALGFESLGLTCILNQDTMLNVILINTSHELDL
                     LVVSGSLYERKLTEETTSIDVIGQELLRNNNVVSLADGVQKAPGVYLLDNQINIRGGT
                     GFTYGAGSRVMVVLDDQPVLSSDRGDVSIHLLPMEIVQQMEVIKGASSVLYGSSAING
                     VVALRTAWPGSEPVNTVSVYNGIFDNPDSAHYKWWGPPPVYSGFNFSHLKQYEKVDLV
                     LSGHASRNNSNLEGQFSNRARLTWKTRMHTGKDNRISYGINGNILLNDEGIFFLFADA
                     DTNLYRAFGGPYDSAFTTLLNWTYRLTTIDPWLNIHDNHDGNHRIRSRFYHTGVTYSD
                     TTSGNSWLQNLDYQYNRAFKDFVITTGVSGYYFHVNDDDLGHHSGFSGGAYLQADQKL
                     FQRLNINAGMREEFYLLDSVDFVAVPIFKAGASYRLWPQTFIRASYGQGFRVPSLVER
                     YAKTKLGILYIFPNPDITAEYGWNAEFAVKKTWSNNYFTGYTDAAFYVTDYYDMTEFI
                     FNQYIGEGIGFKSLNTGRARMAGLELTAAGEGSVFGLPYSIVGGYNY"
     gene            1905..3017
                     /locus_tag="PK742_11995"
     CDS             1905..3017
                     /locus_tag="PK742_11995"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQU77356.1"
                     /translation="MNYCKLTCLLVLGFVLGSCTQQQTAEPGSTLISGKVAGVEGKVY
                     LLMERDHDLVKVDSSGISRGKFTLNAEMPSPEVVFIQLNSSDNLYRLFAEEGNIEIEV
                     DPMQDDLQIVYGSAADQSMREAMEVLKPYDEELNHLFELFKNPKITLNAEVNDSISRL
                     ADDIYARQFEALNSWVMAHGSDPVAAYMVNKHLIYEADYDQLHAWAEMFGRSIPDSRY
                     TQMLTERDGILMQSKVGSPAPDFMLPDADGNPVSLSSLRGQVVLIDFWASWCGPCRRE
                     NPNVVKTYREFHDRGFEILGVSFDNGRDAWLGAVAEDSLSWIHVSDLKGWQNSAGQLY
                     GIISIPHTVLVDTNGIVLAHNLRGEDLRAKLEEILP"
BASE COUNT          784 a          706 c          776 g          762 t
ORIGIN      
        1 gtagttgtaa ccacccacga tgctgtatgg cagaccgaaa acagaacctt ctcctgcggc
       61 tgtcaattcg agacctgcca tgcgggcacg tcccgtattc agtgatttga aaccaattcc
      121 ttctccgata tattgattaa aaatgaactc ggtcatatca taatagtcag ttacatagaa
      181 tgctgcatct gtataaccgg taaagtaatt gttactccag gtctttttga ccgcgaactc
      241 cgcattccag ccatattcag ctgtgatatc cggattgggg aaaatgtaca gaataccaag
      301 tttcgttttg gcataacgct ctaccagact gggtacgcgg aacccttgtc cgtaagatgc
      361 acgaatgaaa gtttggggcc aaagccggta ggatgcaccc gctttgaaaa tgggcactgc
      421 aacaaagtca actgaatcca gcaggtagaa ctcttcccgc ataccggcat tgatattcaa
      481 tctctgaaac agcttttgat cggcctgcaa ataggctcct ccggagaatc cgctatggtg
      541 cccgagatca tcgtcattta catgaaaata gtaaccggat actcctgttg tgatcacgaa
      601 atctttaaaa gcacggttgt actgataatc cagattctgc agccaggagt ttccggaggt
      661 ggtatctgaa taggtcacac ccgtgtggta gaacctgctg cggatgcgat ggttgccatc
      721 atggttgtcg tgaatattca gccaggggtc gatggtggtg agtcggtagg tccagttcag
      781 cagggtggtg aaggcactgt catagggtcc gccgaatgcc ctgtacaagt tggtatctgc
      841 atcggcaaac agaaagaaga tgccttcatc attgagcagg atattgccat tgatgccata
      901 tgaaatgcgg ttgtccttgc ctgtatgcat gcgggtcttc caggtgagac gtgcacggtt
      961 gctgaattgt ccttccaggt tgctgttgtt tctgctggca tgtccgctca ataccagatc
     1021 cactttttcg tattgcttca gatgcgaaaa gttgaaaccg gagtaaactg gaggaggtcc
     1081 ccaccattta taatgcgcag aatccggatt gtcgaaaata ccgttataga cagacacggt
     1141 attgaccggt tcagaacccg gccatgcagt acgcaaggct accacaccgt tgatggcact
     1201 gctgccatac aatacagatg aggcgccttt gatgacctcc atctgctgca cgatctccat
     1261 aggcaggagg tggatgctca catctccgcg atcagaagag agcacgggct ggtcatccag
     1321 taccaccatg acacggcttc ctgcaccata ggtgaatccc gtgccgccgc gaatgttgat
     1381 ctggttatcg agcagatata cacccggtgc tttctgcacg ccatcagcca gacttaccac
     1441 attgttattg cgcagcagtt cctgaccgat cacatctatg gaggtggtct cttcggtcag
     1501 ctttctttca tacaggctgc cactcactac taaaaggtct agttcgtggg aagtatttat
     1561 caggatcacg ttcaacattg tatcctgatt gagtatgcag gtcaacccca ggctttcaaa
     1621 acccagtgca ctgatctgga aaaaatgttc tcctggaggc aattccagat aaaaatgacc
     1681 agattcatca gctgcagtac cttgtccgtt ttcaaccagt atggtagcaa aagggatggg
     1741 tagtttcagc tcatcagaaa catgtccact gatctgaaaa gtctgtgctt tcaacatacc
     1801 tgccagcagc aggataataa gggtaccggt ggttttcata cttagtattt cgaaaatacc
     1861 aaaacgctga caatcggcac attctggcta cgtaccttta ttgcatgaat tattgtaagc
     1921 tgacctgtct gttagtcctt ggttttgtat tgggaagttg cactcagcag cagactgctg
     1981 aacctggctc tactctgatc tccggaaaag tagcaggagt ggagggtaag gtttatctgc
     2041 tgatggaaag agaccatgac ctggtcaagg tagattcatc tggtatctcc agggggaagt
     2101 ttaccctgaa tgcagagatg ccatcgccgg aagtggtgtt catccagctg aacagcagcg
     2161 ataatctgta ccggttattt gccgaagaag gcaatattga gatcgaggtg gatcccatgc
     2221 aggatgacct gcagatcgtt tatggttcag cggcagatca gtccatgcgg gaggccatgg
     2281 aagtgctgaa gccttatgat gaagagctga accacctgtt cgaattattc aagaacccca
     2341 agatcaccct gaatgctgaa gtcaatgaca gcatttcccg gctggccgat gatatctatg
     2401 cccgacagtt cgaggcactg aattcctggg tgatggcaca tggcagtgat ccggtggccg
     2461 cttacatggt caacaaacat ctgatctacg aagcagacta cgatcaactg catgcatggg
     2521 cagaaatgtt cggacggtct atcccggaca gcagatacac acagatgctg accgaaagag
     2581 atggcattct gatgcagtca aaggtgggta gccctgcgcc tgattttatg ctgccagatg
     2641 ctgatgggaa tccggtttcg ctttcttcat tgcggggaca ggtagtgctc attgatttct
     2701 gggccagctg gtgcggacca tgtcgcagag agaacccgaa tgtggtgaag acctacaggg
     2761 aatttcacga tcgcggattt gagatcctcg gagtatcatt tgacaacgga agagatgcct
     2821 ggctgggtgc tgtggcagaa gacagcctca gctggatcca tgtatctgac ctgaagggct
     2881 ggcagaacag tgccggtcag ttatacggca tcatatctat accacataca gtgctggttg
     2941 atactaatgg tatagtgctt gcacacaacc tcaggggaga ggatctgcgg gcgaagctgg
     3001 aagagatatt gccataaaaa aagccggc
//
LOCUS       DAOMAX010000305         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6736_length_3027_cov_4.058210, whole genome shotgun sequence.
ACCESSION   DAOMAX010000305 DAOMAX010000000
VERSION     DAOMAX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6736_length_3027_cov_4.058210"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PK742_12000"
     CDS             complement(<1..734)
                     /locus_tag="PK742_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQU77357.1"
                     /translation="MSDTGTLTAKDFQTDQEVRWCPGCGDYSILKQVHTVLPQLGIPR
                     EDIVVISGIGCSSRFPYYTNTFGMHSIHGRATAIASGLKASRPELSVWIVTGDGDGLS
                     IGGNHMIHILRRNFDVNILLFNNEIYGLTKGQYSPTSPVGKVAKSTPMGSIDHPFNPA
                     ALALGADATFVARSMDRDPQHLQSMLLRANQHHGASFLEIYQNCNIFNDGAFEVFTEK
                     SSKKSNGLFVEHGKPLLFGDNEEYGI"
     gene            complement(756..2594)
                     /locus_tag="PK742_12005"
     CDS             complement(756..2594)
                     /locus_tag="PK742_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQU77358.1"
                     /translation="MELKTLDRVVIKFAGDSGDGMQLTGGQFTNNTALLGADLQTFPD
                     FPAEIRAPAGTLAGVSGFQLHFGSNKIYTPGDQCDVLVAMNAAALKVNIKNLKPGAIV
                     IVNTDGFDSKNLRLAHYDESPLDDHSLDGYQLIRIDVTRMTREALADIELSTKEKDRC
                     KNMFVLGFLFYMYNRKPDSTVRFLESKFKNKPEVLEANIKALQTGINFADTTEIFTNR
                     YSVNAADLEPGTYRSIMGNEAAAMGFIAASQQSGLPLFLGSYPITPASDILHYLSKHK
                     NFGVRTFQAEDEIAAICASIGASFGGQLALTTTSGPGVALKAEAMGLAVMLEMPLVIC
                     NIQRAGPSTGLPTKTEQADLLQALYGRNGECPMPVIAARTPSDAFMAAFEACRIAIQH
                     MTPVMFLSDGYIANGAEPWKFPQAEDLPEIPVTFAPAIEEGETFLPYERNEDLVRPWA
                     IPGTKGLEHRIGGLEKQDVTGNISYDPDNHEHMVKTREEKVERIADHIPLQQIHAGPS
                     KGKVLVIGWGSTYGAIQGAVTRLVNDGHAVAHAHLRYIKPFPKNLEELMRSFDHVLVP
                     EINNGQLVKVIRDRFLIPAIPFNKIKGVPITSAELQAAIMDIINGN"
BASE COUNT          648 a          773 c          848 g          758 t
ORIGIN      
        1 ttgataccgt attcttcatt atcgccaaac agcagcggct tgccgtgttc cacgaacagt
       61 ccgttgcttt tcttgcttga cttttcagtg aagacctcaa aggctccatc attgaagatg
      121 ttgcagttct ggtagatctc gaggaaggaa gcaccgtggt gctggttggc acgcaacaac
      181 atgctctgca ggtgctgcgg atcgcggtcc atacttctgg ccacaaaagt agcatcggct
      241 cctaaagcca gtgctgcggg attgaagggg tgatcgatcg aacccatggg tgtggacttg
      301 gccacttttc ccactgggga tgttggtgaa tactgtccct tcgtcaagcc atagatctca
      361 ttgttgaaca gcaggatgtt cacgtcaaag ttcctgcgca ggatgtggat catgtggttg
      421 ccgccgatgg ataaaccatc cccatcacct gtcacgatcc agacgctgag ctccggacgg
      481 gatgccttca gtccgctggc gatggccgtg gccctgccgt ggatgctgtg cataccaaag
      541 gtgttcgtat agtagggaaa ccgtgagctg cagccaatgc cggagatgac cacgatgtct
      601 tcccgcggaa tacccagctg aggcaacacg gtatgtacct gcttgaggat ggaataatct
      661 ccacatccgg ggcaccagcg tacctcctga tcggtctgaa aatccttggc tgtaagtgtg
      721 cctgtatcac tcataatatc ctgattcgta gtatatcaat taccattaat aatatccatg
      781 atggctgcct gcagttctgc actggtaata ggcactcctt tgatcttgtt gaaaggaatg
      841 gccggaatga ggaaacggtc gcggatcact ttgaccagct gaccattgtt gatctcaggc
      901 accagcacat gatcgaagct gcgcatcagt tcttccaggt tcttcgggaa gggtttgatg
      961 tagcgaaggt gggcatgggc cacggcatga ccatcgttca caagtctggt aaccgcaccc
     1021 tgtatagcac cgtaggtaga accccagcca atcaccagca cctttccttt ggaaggtccg
     1081 gcatgtatct gttggagtgg aatatgatct gcgatgcgtt ccactttttc ttcccgtgtt
     1141 ttcaccatgt gttcatggtt gtcggggtcg tagctgatgt tgcctgttac gtcctgtttt
     1201 tccagtccgc cgatgcggtg ctccagtccc tttgttccgg gtatcgccca gggtcttacc
     1261 aggtcttcat tgcgttcgta tggcaggaag gtctctcctt cttcaatggc cggtgcgaat
     1321 gttacaggta tctccggcag gtcttctgcc tgcgggaatt tccagggctc cgcaccgttg
     1381 gcaatgtagc cgtcgctcag gaacatgacc ggggtcatat gctgaatggc gatgcggcag
     1441 gcttcaaatg ctgccatgaa ggcatcactc ggggtacggg ctgcaatgac cggcatgggg
     1501 cattcgccgt tgcggccgta taatgcctgg agcagatcgg cctgttctgt tttggttggc
     1561 agaccggtac tggggccggc acgctgaatg ttacagatga ccagtggcat ctccagcatg
     1621 accgccagac ccatagcctc tgctttcagc gctacccccg gaccggaggt tgtggtgagt
     1681 gccagttgtc cgccaaagga tgcgccaata gaagcacata tagcagcgat ctcatcttcc
     1741 gcctggaagg tgcgtacgcc gaagttctta tgcttgctca ggtagtggag gatgtcagat
     1801 gccggagtga tgggatagga tcccaggaac aaaggcaggc cggattgctg actggcagcg
     1861 atgaagccca tcgcagccgc ttcattaccc atgatgctgc ggtaggtgcc gggttccaga
     1921 tcggcggcat tgaccgaata acggttggtg aatatctctg tggtatcagc gaagttgatg
     1981 cctgtttgaa gggccttgat attggcctct aatacctccg gcttgttctt gaatttcgat
     2041 tccaggaagc gtaccgtaga atccggtttc cggttgtaca tgtagaacag gaaaccgagt
     2101 acgaacatgt tcttgcagcg gtccttttcc ttggtgctga gttctatatc cgccagtgcc
     2161 tcccgtgtca tgcgggtcac gtctatgcgg atgagctgat aaccgtccag cgaatgatcg
     2221 tccagcggac tctcgtcata gtgtgccagg cgcaggttct tgctgtcaaa gccatcggtg
     2281 ttgacgatga cgatggcacc gggcttcagg tttttgatgt tcaccttgag ggcagcagcg
     2341 ttcatggcca caagcacatc gcactgatcg cccggggtat agatcttgtt gctgccaaag
     2401 tgaagctgaa agcccgatac accggccagg gtacctgcgg gcgcccggat ctcagccgga
     2461 aaatccggga aggtctggag gtctgcaccc aggagggcgg tgttgttggt gaattgtcca
     2521 ccggtcagct gcataccgtc gccactgtcg ccggcaaatt tgatgaccac acggtcaagc
     2581 gtcttgagtt ccatgcacag aatctttcta cagtcctgca aattacaaag caggtcagta
     2641 tcagcaaagg taggggaaag gaaaggagga gaaagatgac ggaagtcgcc atctataata
     2701 aacacaagat cagacaaaag cccgctcgtc tctgtcttac ctgtcattca gtccccacag
     2761 tgtcacccga aggggactct gtggctttcc taatgcaccg ttgatcattt gcaaagttcc
     2821 tttcccacct gcagcaggca agtacactat tccataatgg cttgaaaact gttggggaag
     2881 ttctgatcag gtataagccc gttcgtctct gccttccatg tagttgacaa atgctttgtt
     2941 ggccaccttg ttgccgccgg gggtgggata gttgccggtg aagtaccagt cgccgaggtt
     3001 gtgcggacag gcggtatgca gatcatc
//
LOCUS       DAOMAX010000306         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6783_length_3016_cov_3.770348, whole genome shotgun sequence.
ACCESSION   DAOMAX010000306 DAOMAX010000000
VERSION     DAOMAX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6783_length_3016_cov_3.770348"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1875
                     /locus_tag="PK742_12010"
     CDS             124..1875
                     /locus_tag="PK742_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU77359.1"
                     /translation="MSILSIRDLTVRFAGEQGTSTAVNGISFDLQKGEVLGIVGESGS
                     GKSVTSLSVMRLLSEPGSIDKGSVHFGEDAPVDLLSLPEKEMRHFRGNRISMIFQEPM
                     TSLNPVFTCGFQVDEALMLHKKISRKQAKTETLRLFEQVQLPDPERIYKAYPHELSGG
                     QKQRVMIAMAMSCEPDILIADEPTTALDVTVQQTILKLMLDLQQQNGMSVIFITHDLG
                     VIAEIADRVLVMYKGNIVEQGTVEELFHNPQHPYTKGLLACRPPLDKHLKRLPVIADF
                     MQEQEDGSLHTTPISVQDAITRETISPADREARLQQLQEQDPVLQVKGLKTWFPSGGN
                     WLGGNKHWVKAVDDVSFDVYPGETLGLVGESGCGKTTLGRTILRLQEPTGGQIIYKGR
                     DLLSLSSKEMRDLRKEMQIIFQDPYSSLNPRMTIGAAIREPMTVHGLYSNERERTEKV
                     IDLLEKVNLSADHFGRYPHEFSGGQRQRVVIARALSLNPSFLICDESVSALDVSVQAQ
                     VLNLLIDLRESFGFTYIFISHDLSVVKFMSDRMVVMNKGKIEEMGPADAIYHHPQSAY
                     TQRLIDAIPGGVSKAEV"
     gene            2069..2374
                     /locus_tag="PK742_12015"
     CDS             2069..2374
                     /locus_tag="PK742_12015"
                     /inference="COORDINATES: protein motif:HMM:NF026241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M91 family zinc metallopeptidase"
                     /protein_id="HQU77360.1"
                     /translation="MTVNAEQYVVLSHELLHSKDIWNGALDRSLSKEIAITFDWGQEW
                     VDANTHEVRRTPGFEPKKNSEVNAIKGENKIREEHGLGLRILGVSTKDVTNVLPETE"
     gene            2680..2934
                     /locus_tag="PK742_12020"
     CDS             2680..2934
                     /locus_tag="PK742_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77361.1"
                     /translation="MIRSVSEEELAGLLSYPNFGQSAALSGGPYIDSTEFDHYRVIDF
                     VALQVCIIARGNSIYDDFFRSSGFMTDYWYEDRKQIIPKE"
BASE COUNT          859 a          664 c          735 g          758 t
ORIGIN      
        1 cctgacttga ctatggcacc acgggtacat acctgttggt gctcaactct ttttctctcc
       61 caatagccat cgagacctgt cggggttcca ccccgtttcc acctttacct ctatcttcgt
      121 ctcatgtcca tcttgtccat ccgtgacctc accgtccgct ttgccggcga acagggtaca
      181 tccacagcag tgaatggcat ctcgttcgat ctgcaaaaag gagaagtact gggcatcgtg
      241 ggtgaatccg gttccggaaa atctgtcacc agcctgtcgg tcatgcgttt gctgagtgag
      301 ccgggaagta ttgacaaagg cagtgttcat tttggtgaag atgcaccggt tgatctgctg
      361 tcactgcctg aaaaagagat gcggcatttt cgaggcaacc gcatcagcat gatcttccag
      421 gagccgatga ccagtctcaa tccggttttc acctgtgggt tccaggtaga tgaggctttg
      481 atgctgcaca agaagatcag ccggaagcag gcaaaaacag aaacattgcg attgtttgaa
      541 caggtgcagt tgccggatcc ggaacgcatc tacaaagctt atccgcatga actgagtggc
      601 ggacaaaaac agcgtgtcat gatcgccatg gccatgagct gcgaaccgga catactcatt
      661 gccgacgaac ctaccaccgc tctcgatgta acagtacaac agaccatctt aaaactgatg
      721 ctggacctgc agcaacagaa tggcatgtcg gtcatcttca tcacacacga tcttggcgtc
      781 attgcagaga tcgcagatcg tgtgctggtc atgtacaagg gaaacattgt ggaacagggc
      841 accgtagagg agttgttcca taatccgcag catccttaca ccaaaggact gctcgcttgc
      901 cgcccgccgt tggataaaca cctgaaaaga ttgcctgtca tcgccgactt catgcaggaa
      961 caggaagacg gcagcctgca taccacaccc atcagcgtac aggatgccat tacccgtgag
     1021 accatctctc ctgccgatcg cgaagcaaga ctgcagcaac tgcaagaaca ggatccggtg
     1081 ttgcaggtga aggggctgaa gacctggttc ccttccggag gcaactggct gggaggcaac
     1141 aagcattggg tgaaagcggt ggatgatgtc agctttgatg tgtacccggg tgagaccctc
     1201 ggactggtag gtgaatccgg ttgcgggaag accacactgg gaagaaccat cctccgatta
     1261 caggaaccga ccggcggaca gatcatctac aagggcagag acctgctcag tctttcgtcg
     1321 aaagaaatgc gtgacctgcg gaaggagatg cagatcatct tccaggaccc gtattcttcg
     1381 ctcaatccgc gaatgaccat tggcgctgcg atcagagagc cgatgaccgt acacggattg
     1441 tacagcaatg aaagagaacg aaccgaaaag gtcattgacc tgctggaaaa ggtcaatctg
     1501 agtgccgatc attttggaag atatccgcat gagttcagcg gcggacaaag acagcgggta
     1561 gtcattgccc gggcattgtc tttgaaccct tcttttctca tctgcgatga atcggtttca
     1621 gcactcgatg tatccgttca ggcacaggtg ctcaacctgt tgatagacct gcgggaatct
     1681 ttcggcttta cctacatctt catcagccac gatctcagtg tggtcaagtt catgagcgac
     1741 aggatggtgg tgatgaacaa gggaaagata gaagaaatgg gaccggccga tgccatctac
     1801 caccatccac agtcggccta cacacagagg ctcattgatg ccataccggg aggagtttct
     1861 aaggcggaag tctaaggtac aaaccagatg ttcaggtatt agatttgaat gaacaatcgc
     1921 aagaagagaa ctaactgagt actttggaga ccggcattct aacgctgaca gagttagttg
     1981 aacactcccg attggggcgg gatctatagt ttatttcccg actgattatc aatctccatc
     2041 caaaatagta aatgagtctg gcagccttgt gacagttaat gctgaacaat acgtagttct
     2101 ttcccatgag ttgcttcatt ctaaggatat ttggaatggg gctttagatc gctctttgtc
     2161 aaaggaaatt gcaataacat ttgattgggg acaagaatgg gttgatgcaa atacgcatga
     2221 agtgcgtcga actcctggtt tcgaaccaaa gaagaatagc gaagtaaatg caattaaagg
     2281 agagaataaa atacgagagg agcatggatt aggtctacgt atattgggtg ttagcacaaa
     2341 agatgtaaca aatgtattac cagaaactga gtaaaggggc tcaaatagca gggttattct
     2401 taatgctatt tcaaattacc ccaagattat caacggctca aaatcaacct gattcttgtt
     2461 taagtcaagg atacttatca gtttcagacg aaataagatt agatgctgca ttaatagaag
     2521 aggtggtaaa tcttttcagt cccagaatgc ccagatgtta tttcaattat atactcacaa
     2581 gatgaacatc atagctatat tcatatcata tcaaatgtat tttatacaga tagtaaatca
     2641 tatactatat tcagtgatta ctcaaaagaa tacagggcaa tgatcagatc ggtatcagaa
     2701 gaggaacttg ctggcttgct aagttatcct aattttggtc agtcagcagc gctttcgggt
     2761 gggccttata ttgattcaac ggagtttgat cactatcgag taatagattt tgttgcactg
     2821 caagtatgta taatcgctcg aggcaatagt atatatgatg atttttttag atcgtcagga
     2881 tttatgactg actactggta tgaggataga aaacaaatta taccaaagga atagctcagt
     2941 acatcataaa catgttcata gtatatgggt catatattat actagataat gattgttatc
     3001 cctttgggat gatgat
//
LOCUS       DAOMAX010000307         3005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6829_length_3005_cov_3.778305, whole genome shotgun sequence.
ACCESSION   DAOMAX010000307 DAOMAX010000000
VERSION     DAOMAX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6829_length_3005_cov_3.778305"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK742_12025"
     CDS             <1..419
                     /locus_tag="PK742_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77362.1"
                     /translation="EIICEAQQFYIPIGTDPEYDYHIYSGIDDREPLTELPGVLNNIF
                     FRPNVIHSSSLVRRYFALELMLCWIVLLMLMIQSASRRYHISPTTWLLTWLSVAVLLL
                     YGYVVTDADTMSRYRSIPVFLLLVAWVLPGERKLEG"
     gene            817..>3005
                     /locus_tag="PK742_12030"
     CDS             817..>3005
                     /locus_tag="PK742_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQU77363.1"
                     /translation="MQISRSNALQHILNRGSKEVKFPGTRISDYYGTNVFNDEAMRKH
                     LSEEAYLSVKASIQQGEKIDRKIADQVASSMKAWAIEKGATHFTHWFQPLTGATAEKH
                     DSFFVPTEDGKGIESFTGSSLVQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFIME
                     IGSGKTLCIPTIFVSYTGEALDNKAPLLKSLHFLETAAVPVCQYFDKNVSRVSATLGW
                     EQEYFLVDEALFHARPDLMLSGRTLVGHAPAKGQQLEDHYFGSIPERTYNFMLDFETE
                     CHKLGIPVRTRHNEVAPGQFECAPMFEECNLAVDHNILLMDVMEKVARKHKLRVLFHE
                     KPFAGVNGSGKHNNWSMGTDTGKNLLSPGKTPRTNLQFLTFFVNTIKAVHEGADLLRA
                     AIASASNDHRLGANEAPPAIISVFIGTALRAVLEEIENRVDEGGIDELENADLKLDIH
                     NKIPDLMLDNTDRNRTSPFAFTGNKFEFRAVGSTANCASAMTVLNCIVANQLKAFREE
                     VDGMIKGGEKKDNAILMVLRRYISESKAVLFEGDNYSEEWHKEAAKRGLNNIKTTPEA
                     LDQYLTEKANKVFVDNKIFTKRELHARYEVEMEAYAKKIQIEARVYSELVYNYIMPAA
                     VAYQNELLDNITRLKTAGVAETGYRAQLDMVNQISQYINNMRNSLELMIDQRRKANEL
                     EDAAKKAHAYCEKVKPLFDDIRKNADYIERYCPDMHWPLPKYRELLFV"
BASE COUNT          767 a          808 c          780 g          650 t
ORIGIN      
        1 cagagatcat ctgcgaagcc cagcaattct atattccgat agggacagat ccggaatacg
       61 attatcacat ctatagtggt atcgatgata gggaaccatt gacagaactt ccgggtgtgc
      121 tgaataacat cttcttccgg ccgaatgtca ttcatagttc ctcactggta aggcgttact
      181 ttgccctgga actgatgctc tgctggatcg tgctgctgat gctgatgatt cagtcggcat
      241 cgaggcgtta ccacatctct cccaccacct ggctgctcac ctggttgtcg gtggcagtac
      301 tactcctgta tggctacgtg gtgaccgatg ccgataccat gagccggtac cggtccattc
      361 cggtgttttt gctgctggtg gcgtgggtgt tgccagggga gcggaaattg gaaggttaaa
      421 tgtttggtag ccccataggt ggggattttt ttttgtcacc catagcgtta gccacagagt
      481 tccttgtccc tcctccgtcg ggcaggcagg taactctgtg gggacggaag ggttaaccaa
      541 caaccaacaa ccaataacca aaaactaata accagtagcc aacaactaac taccctgcat
      601 ctcttcgcga ttcgcacatt caactgcttt tgggccccga aatcgctgcc agcgaccctt
      661 tattgccgat ttcataccat ttaatttata tttttcgata tattagttat ttttgggcag
      721 gtgggctgtt tttgcacatt tgttgaaata ttttgcacat attttggaga atggtagcaa
      781 attatcggag ttttgtacca gaaaaatccc aaaataatgc aaatatcccg ctccaacgcc
      841 ctgcagcaca tcctgaacag gggctcgaaa gaggtgaaat ttcccggcac ccggatctcc
      901 gactactacg gcaccaatgt gttcaacgat gaggcgatgc gcaagcacct tagcgaggaa
      961 gcctatctga gtgtcaaggc atccatccag cagggcgaga agatcgatcg caagatcgca
     1021 gatcaggtag cctccagcat gaaagcctgg gccatcgaaa aaggggctac ccacttcacc
     1081 cactggtttc agccgctcac aggcgccaca gccgaaaagc acgacagctt cttcgttccc
     1141 actgaagacg ggaaaggcat cgaatccttt accggatcat cgctggtgca gcaggaaccc
     1201 gacgcttcca gtttcccgag cggaggcatc aggagcactt ttgaggcccg cggctatacc
     1261 gcctgggatc cctcctcccc tgctttcatc atggagatag gctccggcaa gaccctttgt
     1321 atccccacca tcttcgtatc ctataccggc gaggcgctgg acaacaaagc gccgctgctg
     1381 aagagtctgc attttctgga aacggctgct gtgcctgttt gccagtattt tgacaagaac
     1441 gtgagcaggg tgagtgccac cctgggctgg gagcaggaat atttcctcgt ggatgaagcg
     1501 cttttccatg cccgtcccga cctgatgctg agcggccgca cgctggtggg ccacgcaccg
     1561 gctaaagggc agcagctgga agaccactat ttcggaagca tcccggagcg cacctacaat
     1621 ttcatgctcg atttcgagac cgagtgtcat aagcttggca taccggtccg cacccgccat
     1681 aatgaagtag cacccggaca attcgagtgt gcccccatgt tcgaagaatg taatctggcg
     1741 gtggaccata acatcctgct gatggacgtg atggaaaagg ttgcccgcaa gcacaagctc
     1801 cgcgtactct tccacgagaa gccatttgca ggtgtgaacg gaagcggcaa gcacaacaac
     1861 tggagcatgg gcaccgatac cggcaagaac ctgctcagtc cgggcaaaac tccacgcacc
     1921 aacctgcagt tcctgacctt ctttgtgaat accatcaagg ccgtgcatga aggcgccgac
     1981 ctgcttcgtg cagccattgc atctgccagc aacgaccacc gcctgggtgc caacgaggct
     2041 cctccggcca tcatatctgt attcattggt actgcacttc gtgccgtgct cgaagagatc
     2101 gagaaccggg tggacgaagg tggtatcgac gagctggaga acgccgatct gaaactggac
     2161 atccataata agatccccga tctgatgctg gacaatacag acaggaaccg gacctcaccg
     2221 ttcgcattca ccggtaataa attcgaattc cgggcagtgg gcagcaccgc caactgcgcc
     2281 agcgccatga ccgttctgaa ctgtatcgtt gccaaccagc tgaaagcctt ccgcgaagaa
     2341 gtggatggca tgatcaaggg tggtgaaaag aaagacaacg ccatcctgat ggtgttgcgt
     2401 cgctacatca gcgaaagcaa agccgtgttg ttcgagggcg acaactacag cgaagaatgg
     2461 cataaggaag ctgcgaagag agggctcaat aatatcaaga ccacaccgga agccctggac
     2521 cagtacctga ccgaaaaagc caacaaggtc tttgtggaca ataagatctt cacgaagcgt
     2581 gaactgcatg cccgctatga ggtggagatg gaagcctatg ccaaaaagat ccagatagaa
     2641 gcaagggtgt acagcgaact ggtgtacaac tacatcatgc cggcagctgt ggcttatcag
     2701 aatgagttac tggacaacat caccagacta aaaactgctg gtgttgccga gaccggttac
     2761 cgtgcacagc tggacatggt caaccagatc agccagtaca tcaacaacat gcgcaacagc
     2821 ctggaactga tgatcgatca gcgccgcaag gccaatgagc tggaagatgc ggccaagaag
     2881 gcacacgctt actgcgaaaa ggtgaagccg ctgttcgatg acatccgcaa gaacgccgac
     2941 tatattgagc gctactgccc cgacatgcac tggcccctgc cgaagtacag ggagttgttg
     3001 tttgt
//
LOCUS       DAOMAX010000308         2983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6928_length_2983_cov_4.463456, whole genome shotgun sequence.
ACCESSION   DAOMAX010000308 DAOMAX010000000
VERSION     DAOMAX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6928_length_2983_cov_4.463456"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="PK742_12035"
     CDS             <1..1986
                     /locus_tag="PK742_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQU77364.1"
                     /translation="MFDEIMVIFVAVMLTDILLLDFFNTFGLPTSTTVSIVFELLGSA
                     TAIAAYKIVTENGSWSSLGDYINQEQAIFIISGIFISVLVAFAVGLVVQYLTRLIFTF
                     DYEKQMQKFGGIFGGIAITVIVDFILLKGAKGTSFMPASAVTWIEQNTMLINGVGFIV
                     WTIVCQIAIVVFRMNILRFIVLLGTFALAMAFAGNDLVNFIGVPIAGLISYQHWAGSG
                     VAPDALGMDFLATSVRTDTYLLVLAGTIMTLTLIFSRKARSVTATSVDLGRQGLGTER
                     FRPNWLSRMIVGGSISLGNAGRSIMPGSVNKGIEKRFSNSDGLSGGEKDKPAFDLVRA
                     SVNLMVAAVLIAFATSLKLPLSTTYVSFMVAMGASLSDRAWNRDSAVYRVAGVVNVIG
                     GWFLTAIVAFSCSALVALFIINTGVIGLLLVGALVLFFVIRSSRIHKKMEAEKDESWA
                     GIIDGNQIELDVLKTETAGYIRSTFKLIYDLYEDLIQTLEAEKSGHIDRYRKELKKLR
                     KQVRDVQTSMYVRLQKLNTGGDAGLRYVELMHELQNVQQPIQLIATMTIDHLRNFHHP
                     VAESQLEELDKVRIAMLEHARLWQHALDESDPDIQWSTDSTLERIIDECLSRQIDAVR
                     TGESGYRNSLLVNSLLLESKNLRLHAERLLAAYRKMK"
     gene            2036..>2983
                     /locus_tag="PK742_12040"
     CDS             2036..>2983
                     /locus_tag="PK742_12040"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQU77365.1"
                     /translation="MEWQAIITLLVILGAIILFVSEWISVDLVALLIMVTLVITGVVT
                     PRESVEGFSNNATITVAFMFVLSEALLRTGALQFVANRLSSIFRKRYLAGMALMMLLV
                     AVISAFVNNTPVVAVFIPVVIQISRSAGISPSKLLIPLSFATIFGGTCTLIGTSTNIL
                     VAGILEKEHVGSISMFDMAPMGLVFLSAGIIYMVVVGFRLLPDRGQEKDLRQKFSMRD
                     YLTEMEIPAGSDLAGKRIMDAGIFSELEMDILDLRRGGDSYPVPSGDFLLQAGDVLKV
                     RCNVEKIRGLKDRMRIMEKPQVTIGEDDLTGKNSVLVEMV"
BASE COUNT          722 a          713 c          770 g          778 t
ORIGIN      
        1 atgttcgatg agatcatggt cattttcgta gcggtcatgc tgacagatat cctgctgctt
       61 gacttcttca ataccttcgg ccttcccacc agcacaacgg tatctattgt atttgaactg
      121 ctgggatcgg caactgctat tgcagcctac aagatcgtta cggagaatgg ttcctggtca
      181 tcactgggtg attatatcaa tcaggaacag gcaatattca ttatatcagg tatattcata
      241 tctgttctgg tggcattcgc agtgggtctg gtagtgcagt acctgacccg cctgatcttc
      301 acatttgact atgaaaagca gatgcagaag ttcggaggca tattcggtgg catagccatc
      361 accgtcatag tggatttcat cctcctgaaa ggcgccaaag gcacatcatt catgccggca
      421 tctgcagtta cctggatcga gcagaatacc atgctgatca atggtgtggg atttattgta
      481 tggaccatcg tctgccagat agccatcgtg gtgttccgaa tgaatattct tcggttcatc
      541 gtcctgcttg gcacgttcgc actggccatg gcttttgccg gtaacgacct ggtcaatttc
      601 atcggagttc cgatagcagg tttgatctcc tatcagcact gggcaggttc cggggttgct
      661 ccagatgccc tgggaatgga cttcctggct acatcggtca gaacagacac ctacctgctg
      721 gtacttgctg gtaccatcat gacccttacc ctgatattca gcagaaaagc ccggtctgtt
      781 accgctacca gcgtggacct gggcaggcag gggcttggca ctgaaaggtt ccggcctaac
      841 tggctgtcca gaatgattgt tggcggcagt atctcactgg gaaatgcagg aagaagcatc
      901 atgccgggat cggtcaacaa gggcatagaa aaacgcttca gtaattcaga cggtttatcc
      961 ggaggagaaa aagacaaacc tgctttcgac ctggtacggg cctctgtgaa tcttatggtg
     1021 gctgcggtgc ttatcgcatt cgccacatcc ctcaagcttc ctctttcaac tacctatgtc
     1081 agcttcatgg ttgccatggg agcttcctta tctgaccgtg cctggaaccg cgattcagct
     1141 gtttaccgtg tagccggtgt ggtcaatgtg ataggggggt ggtttcttac tgctatcgta
     1201 gcgttctcat gttctgcact cgtggcgttg ttcatcatca acacaggtgt aattggcttg
     1261 ctgctggtag gcgcactggt gctgttcttc gtgatcagaa gcagccggat ccataagaag
     1321 atggaagccg aaaaagacga aagctgggcc gggatcatag atgggaacca gatcgaactg
     1381 gacgtactta aaaccgaaac tgcaggatac atcaggtcaa cttttaaact gatctatgat
     1441 ctgtatgaag acctgataca aacactggaa gcggagaaat cagggcatat tgaccgctac
     1501 agaaaagaac ttaagaaact ccgtaagcag gtcagggatg tacagacctc tatgtatgtg
     1561 cgcctgcaaa agctgaatac aggaggtgac gccggcctgc gctatgtgga attaatgcat
     1621 gaattgcaga atgtgcagca gcccatacaa ctcattgcta caatgaccat tgatcatctg
     1681 cgcaatttcc atcaccctgt agctgaatct cagcttgaag aactggacaa ggttcgaata
     1741 gccatgctgg aacacgccag gttatggcag catgcgcttg atgaatcaga cccggatatt
     1801 cagtggagta cagattctac cctggaacgc attattgacg aatgtctttc acgtcagatc
     1861 gatgctgtca gaaccggcga gtcaggatac cgaaattcct tactggtcaa ttcgctcctg
     1921 ctggaaagca agaaccttcg cctgcatgct gagcgtctgc tggctgccta ccgcaaaatg
     1981 aaataaactt accagtttgc ttgtacagca tcattagagg tgtacatttg cgcccatgga
     2041 atggcaggca atcatcaccc ttctggtcat actcggtgcc atcattctct ttgtttctga
     2101 atggatctct gttgacctgg tggctctgct catcatggtc accctggtca taaccggagt
     2161 ggtcacccca cgtgagagtg tagaaggttt ttccaataac gctaccatca cggtggcctt
     2221 tatgttcgta ctcagcgaag cactgttgcg aacaggtgct ctccaattcg tggccaacag
     2281 attgtcttcc atcttcagaa agcgatacct ggcaggcatg gcactgatga tgctgctggt
     2341 cgctgtcatc tcggcctttg tcaataacac accggtagtg gcggtcttca ttcctgtggt
     2401 catacagatt tcacgaagcg caggtatcag tccgtccaaa ctattgatcc cattgtcatt
     2461 tgctaccatt ttcggaggca cctgtaccct catcggcaca tcaactaata ttttagttgc
     2521 aggcattctg gagaaggagc atgtgggcag tatcagcatg tttgacatgg cgcctatggg
     2581 actggtattt ctctcggccg gtatcattta tatggtcgtt gtgggattcc ggttgctgcc
     2641 ggatcgcgga caggaaaagg acctccggca gaaattcagc atgcgtgatt acctcactga
     2701 aatggagatt cctgccggat ctgacctggc agggaagcgc atcatggatg caggaatctt
     2761 ttccgaactg gagatggata ttctggatct gcgaagggga ggggacagct accctgttcc
     2821 ttcgggcgat tttttgctcc aggccggcga tgtactgaag gtcagatgta atgtggagaa
     2881 gatccgcgga ctaaaggacc gcatgcgcat catggaaaaa ccccaggtca ccatcggcga
     2941 agatgatctg acaggcaaga acagtgtatt ggtagagatg gtc
//
LOCUS       DAOMAX010000309         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6933_length_2982_cov_4.187222, whole genome shotgun sequence.
ACCESSION   DAOMAX010000309 DAOMAX010000000
VERSION     DAOMAX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6933_length_2982_cov_4.187222"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..767
                     /locus_tag="PK742_12045"
     CDS             36..767
                     /locus_tag="PK742_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQU77366.1"
                     /translation="MALLPWQTAVITRVTQETANTRRFFFEIKDSGPFVFRPGQFVTL
                     DLPIHDKQSKRMRSYSVASWPDGSNTFELVIVLMEGGAGTTYLFKEGKPGMEIPFRGP
                     LGHFCLPDTLERDICMICTGTGIAPFRSQVQYIRHAQVPCRDIHIIFGTRKIGDILYR
                     SELEQLSRDMPCLHYHVALSRETPESWNGHIGYVHDIYEKVCENGQKDMDFYLCGWKN
                     MVDEARNRLKDMGYDKERIHLELYG"
     gene            767..2632
                     /locus_tag="PK742_12050"
     CDS             767..2632
                     /locus_tag="PK742_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77367.1"
                     /translation="MFLPVLLIPLAAWVWSRLLISRADGMAGTWSRTLILLSGWMVCI
                     TEGLSAGYALEQQWLMVAWVAFLLTGILLLKDRTLLWTFTLPAIGKQERWFAGWILLV
                     LMLTGVLACVAYPNNWDSMTYHLTRVEHWMQRQTVIHYATNNVRQVSQPPLAEYWILH
                     ILSLGGIDRLANLVQWTAWLISMISIWGILGVWGMSRKVQWSGVLLAAALPMAILQST
                     STQNDLLAAAMMLQSVYWAFRVRNGNRQAVWWMLTAFSLAVFTKGSNYIYLLPVLPFA
                     AIWVLRQFGWSCWKPALFGLAVLLVFNGPHWVRNHDTFGRWTGSDKTLVNDSHDPNQM
                     LAVFLQNLAMECQTPISGWNRALTNAVMEIEKGTGVLPGEPTINWAPSPSFSVGMSSL
                     HEDYTGNLLMVILSIVSLFILAGRYRKHPAVTLALMVVSMAVLFSLLLKWQVWHNRLH
                     LPMLLLFIPAVAVALQYLHQHVRTGILIVVAAASIPWLVFNQSRPMVGKESVFRHYDY
                     YQYFFNQPELLRTFLQLAAVADRNQVEKLGWATSGDAWEYPMWRILQDQEPEMRHVLV
                     GNETRVFERSAWQPEGIVSNRAGLVALPRLQCHGRNYYRTYTDGQWAFFLPEPNL"
     gene            complement(2622..>2982)
                     /locus_tag="PK742_12055"
     CDS             complement(2622..>2982)
                     /locus_tag="PK742_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77368.1"
                     /translation="FCWQAFLQLDRPGGQLSLYPHRYMLIMSRYLGFEPHSGPGHFFD
                     MLEGHFSDSIPRHTHYMEAELSSAFRSLLSDDTQFRPTANTRRSLLHYLIIYYQLHLS
                     GFGKMHSPDILEEVLRD"
BASE COUNT          718 a          713 c          796 g          755 t
ORIGIN      
        1 atttcctaaa cttgtagaac actaaaacct gtgtaatggc cctacttccc tggcagactg
       61 cggtgatcac cagggtcacc caggagactg ccaatacccg gcggttcttt tttgagatca
      121 aggacagcgg cccttttgtg ttcaggccag gccagttcgt taccctggac cttcctatcc
      181 atgataagca aagcaaacgc atgcgcagtt attctgttgc atcctggcct gatggcagca
      241 atacgtttga actggtgatc gtgctgatgg aaggcggtgc cggaaccacc tatctgttca
      301 aagaaggaaa acccggaatg gaaattcctt ttcgcgggcc attagggcat ttctgcctgc
      361 ctgataccct tgagcgtgat atctgcatga tctgcaccgg aaccggtatt gctccgttcc
      421 gcagtcaggt tcagtacatc cggcacgctc aggtaccctg tagagacatc catatcatat
      481 ttggtacaag aaagattggc gacatactct accgttcaga actggaacag ttgtcaagag
      541 atatgccttg tttgcactac catgtagccc tgagccgtga aactccggaa tcatggaacg
      601 gtcatattgg atatgtacat gatatatatg aaaaagtgtg tgaaaatgga caaaaggata
      661 tggatttcta tctgtgtggc tggaagaata tggtagatga agccagaaac cggctgaaag
      721 atatggggta tgataaggaa cgcatccacc tggaactcta cggttgatgt ttttaccggt
      781 tctgctgata ccattggcgg cctgggtctg gagccgcctt ttgatctccc gtgctgatgg
      841 catggcaggc acctggtctc gtactttgat cctgctttcg ggttggatgg tctgtatcac
      901 agaaggactt tctgccggtt atgctttaga acagcaatgg ctgatggtgg cctgggtggc
      961 ctttctgttg accggcatcc tgctgctgaa agaccggacc ctgctgtgga ccttcacact
     1021 gccggcaata gggaaacagg aacgttggtt tgccggctgg atcctgcttg tattaatgct
     1081 tactggtgtg ctggcctgtg tggcttatcc taataactgg gatagcatga cctatcacct
     1141 gacaagggtc gagcactgga tgcaaagaca aacggtcatc cactatgcca ccaataatgt
     1201 acgccaggtg tcacagcctc cgctggctga gtactggata cttcatatcc tgtcgcttgg
     1261 tggtatagac cgtttggcta acctggtgca atggactgcc tggttgatca gtatgatcag
     1321 catttggggg attcttggag tgtggggcat gagcagaaag gtgcaatgga gcggagtatt
     1381 gctggcagcc gccctgccca tggccatcct gcaaagcacc agtacacaga atgacctgct
     1441 ggcagcagcc atgatgctgc aaagtgttta ctgggccttt cgtgtacgaa acggaaacag
     1501 acaggcggtc tggtggatgc tgacggcatt cagcctggct gtgttcacca aaggcagcaa
     1561 ctatatctac ctgttaccgg tattgccttt tgcggccatc tgggtgctcc ggcaatttgg
     1621 ctggtcctgc tggaaaccgg ccttgttcgg tcttgcggtg ttgttggttt ttaatggccc
     1681 gcactgggtg cgtaaccacg atacatttgg ccgatggaca ggcagtgaca agaccctggt
     1741 aaatgacagc catgatccta atcagatgct ggcagttttt cttcagaacc tcgccatgga
     1801 atgtcagacc ccgatatccg gatggaacag ggcacttacc aatgctgtaa tggaaataga
     1861 aaaaggaacg ggtgtgctgc cgggtgaacc caccatcaac tgggccccaa gcccttcctt
     1921 ttcagtaggc atgtcttctc tgcatgaaga ctataccgga aacctgctga tggtcatatt
     1981 gtctattgtc tccctattca tattagcggg aagataccgg aaacaccctg ctgtcacact
     2041 ggctttgatg gtagtatcta tggcagttct ttttagctta ttgctgaagt ggcaggtctg
     2101 gcacaaccgc ctgcacctgc ccatgctgtt attgttcata ccggcagtgg cagtggcttt
     2161 gcaatacctg catcaacatg tacgaaccgg aattctgatc gtggtggcag cagcttccat
     2221 accgtggctt gtttttaacc agagcagacc catggtaggt aaggaaagtg ttttccgcca
     2281 ttacgactat tatcagtatt tctttaacca gccggaatta ctacgcacct ttttgcagct
     2341 ggcagcggta gcagaccgca accaggtgga gaaattggga tgggccactt ccggcgatgc
     2401 atgggaatat ccgatgtggc gtatcctgca agaccaggag ccggaaatgc gacatgttct
     2461 ggtaggcaat gagaccaggg tctttgaaag aagtgcctgg cagccggaag gtatcgtttc
     2521 caaccgtgcc gggctggtgg cactgcccag attacaatgc catggaagaa attattaccg
     2581 gacctataca gacgggcaat gggccttttt tctgccggaa cctaatctct gagtacttct
     2641 tccaggatgt caggcgaatg catcttaccg aatccgctca ggtgcaattg gtaatagatg
     2701 atcagatagt gcaacagtga ccgacgggtg ttggcagttg gccggaattg agtatcatct
     2761 gacaatagtg aacgaaaagc cgatgacaat tcagcttcca tatagtgagt atgccgtggt
     2821 atggaatcac tgaaatgacc ctccagcata tcaaaaaaat gtccgggtcc cgaatggggc
     2881 tcaaagccca gatagcggct catgatcagc atatacctgt gcggatacaa cgacaactga
     2941 cctccgggcc ggtccagctg tagaaaagcc tgccagcaga at
//
LOCUS       DAOMAX010000310         2977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6954_length_2977_cov_3.914784, whole genome shotgun sequence.
ACCESSION   DAOMAX010000310 DAOMAX010000000
VERSION     DAOMAX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6954_length_2977_cov_3.914784"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2977)
                     /locus_tag="PK742_12060"
     CDS             complement(<1..>2977)
                     /locus_tag="PK742_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQU77369.1"
                     /translation="PPDGDFTRKEKATFTYPLPDHTRLALEYATTKGRDGKGCAVFFA
                     AGNGDEDIRHDGYASSPLVLSVGAVNNQGKKARYSDFGDALFCCFPSGDFELGPDGWK
                     QTSGVLVPDRLGADGYDPGDLYTMFAGTSASCPGAAGVAALALSLKPEMRLKDLKELL
                     KNACVPPGEHQHDTRSHHPSFGFGLLRADLAVRQITEQKKYNMNTTATGYSLHIGIDN
                     VDPAYYQNDVPALSGCVNDMRSMQELAAGYGYQTTTLVNSEATREAILAYLKNMAFKT
                     EPGDILLITYAGHGAPIPDTDENGSVKDETDGFDESWVTYSGFLLDDELYAAYAGFKE
                     GVRILVVSDSCHSGTMLRAMISSSWMETMEAERGVRLRYVPLESVRKILARQGGMRSV
                     RSAEMKVAVPEDVKASLKLLAACQDNQYAQEKNGQGLFTVTLLDTLKQTAGNITTYSA
                     LMERVISNMPPYQEPRLTNEGMASPAFDAQLPFSIAGEQKEPVAPVAAKPVPVKRSED
                     VNLLVEDTDTYILGQQIQDETGRNKVLSVVDGEVNGHELSGKTAWDKAYLLALANADD
                     PIGFVEPDMDSDIGSHPVEEVLERGGDSIAEKYDRLKTYPPARDDKTVPFDWHLGNAY
                     SQLKAAREEVFPELLTGDKRSKNKKYVKIAHIDTGFLDGHPAHPEYLDRMHSASFAGW
                     GAGTGGQDTDMKIAPGEQQGHGNATLAILAGGKLRSEQTDGLFSGYFGAIPFADVISI
                     KISESVVLLSGKRFAKAIDHAIDQGVDVITMSMSGWPTKKMARAINRAYENGIVVVCA
                     GSNSWVKQAGFTSHLPDTLLYPARYERTIAAVGATYTHVPYVYALHNRQVRAEGGKYM
                     QMSYGPESAMKTAIAGYTPNVPWFSHMNDRSFPHYYTLNGGGTSSATPQVAAAAALYI
                     QKYRTELDQIAGKDKWKIVEIVKAALFRSAKLDPQYRKYYGNGILKAKDALAISPASV
                     VQHIKLSPEASANRSFVGNL"
BASE COUNT          610 a          811 c          759 g          797 t
ORIGIN      
        1 cagattgccc acaaaggatc ggttggcgct ggcctccggc gacagtttga tatgctgtac
       61 cacggatgca ggtgaaatgg ccagtgcatc tttcgctttc aggataccat taccatagta
      121 ctttctgtat tgcgggtcga gttttgcgga acggaacaat gccgccttga cgatctccac
      181 gatcttccac ttgtctttac ctgctatctg gtcgagttct gtgcggtatt tctggatgta
      241 taatgcagca gctgcagcta cctgtggggt agcgctggaa gtgcctcctc cgttaagggt
      301 atagtagtgc ggaaaggatc tgtcgttcat atggctgaac cagggcacat tgggtgtgta
      361 tcctgcaata gcggtcttca tagcagattc cggaccatag ctcatctgca tatatttccc
      421 accttctgca cgcacctgcc tgttgtgcag tgcatacaca tacggcacat gggtataggt
      481 agctcccact gcggcgatgg ttctttcata gcgggcggga tacagcaaag tatccggaag
      541 atggctggta aagccggcct gtttgaccca ggaattgctt cctgcgcaaa ccaccacgat
      601 cccgttctca taagccctgt tgatagccct ggccattttt ttggtgggcc atccggacat
      661 gctcatagtg atgacatcca ctccctggtc gatggcatgg tctatggctt tggcaaatct
      721 ctttccactt agcagtacga cagactcact gatcttgatg ctgatcacat cagcaaaagg
      781 aatggcaccg aagtatccgc tgaataagcc atcggtctgt tcactgcgta acttgccacc
      841 tgccaggatg gccagtgtcg cattgccgtg gccctgttgt tcgccgggag caattttcat
      901 gtctgtatcc tgtccaccgg taccggcacc ccaaccggca aaggaagctg aatgcatccg
      961 gtccagatat tccggatggg ccggatggcc atccaggaat cccgtatcta tatgggctat
     1021 ttttacatat tttttattct tacttctttt atcgcctgta agcaactccg gaaagacctc
     1081 ttctctggcc gctttcagct ggctgtatgc attacccaga tgccagtcga aaggaacggt
     1141 cttgtcatca cgggccggcg ggtaggtttt caggcgatcg tatttttctg ctatgctgtc
     1201 gccgccgcgt tccagcacct cttctaccgg atggctgccg atatctgaat ccatatccgg
     1261 ttccacaaaa ccgatgggat catcggcatt ggccagtgcc agcagatagg ctttatccca
     1321 ggctgttttt cctgaaagct catgcccgtt cacttcccca tccaccactg acagcacttt
     1381 gtttcgtcct gtttcgtcct ggatctgctg acccagtata taggtatctg tatcttccac
     1441 cagcaggttc acatcctccg atcgcttcac tggtaccggc ttggccgcta ccggtgctac
     1501 cggctctttt tgctcacctg ctatggagaa aggcagctgc gcatcaaatg ccggacttgc
     1561 cattccttcg ttggtaagac ggggctcctg atagggtggc atgttgctga tgaccctttc
     1621 catgagtgcc gaataagtgg tgatgtttcc agctgtttgt ttcagtgtat ccagcagggt
     1681 cacggtgaac aatccctggc catttttttc ctgggcatac tggttgtcct gacaggcagc
     1741 cagaagtttc agtgatgctt tcacatcttc gggtactgcc accttcattt cagctgatct
     1801 taccgagcgc atgccacctt gccgagccag tatcttccgg acagattcca atggtacata
     1861 cctgagtctg acacctcttt ctgcttccat ggtctccatc catgaggaag agatcatagc
     1921 ccggagcatg gtaccgctat ggcaggaatc tgataccacc aggatgcgca caccttcttt
     1981 gaagccggca taggcggcat acaattcatc atcgagcaga aatccgctgt aggtgaccca
     2041 ggattcatca aaaccatccg tctcgtcttt taccgatcca ttctcatcgg tgtcaggaat
     2101 gggagcacca tgaccggcgt aggtgatcag caggatatcg ccgggctctg ttttgaacgc
     2161 catgtttttc aggtaagcca gtatggcttc acgggtagct tcgctgttca ccagagtggt
     2221 tgtctgatag ccgtatcccg ctgccagttc ctgcatgctg cgcatgtcat ttacgcatcc
     2281 ggataaggca ggcacgtcat tctggtagta ggccggatcc acgttgtcga tcccgatgtg
     2341 cagggaataa cctgttgctg ttgtgttcat gttatatttt ttttgttctg ttatttgtct
     2401 gacggccagg tctgcccgca gcagtccgaa accgaatgaa ggatgatggg atcgtgtatc
     2461 atgctggtgt tcgccgggag gtacacaagc gttcttcagg agttctttca ggtctttcag
     2521 ccgcatttcc ggcttcagag acagggccag ggcggccacc cctgctgcac ccggacagga
     2581 agcagatgtg ccggcgaaca tggtgtacag atcgcccgga tcatatccgt ctgctcccag
     2641 cctgtcgggc accagtaccc cgcttgtctg cttccagcca tcgggtccca gctcgaaatc
     2701 gccgcttggg aaacagcaaa aaagtgcatc accaaaatcg ctgtaccggg ccttttttcc
     2761 ctgattgttg acagcaccaa cggagagcac cagcggactg gacgcatagc catcatgccg
     2821 gatatcttca tcgccgtttc cggcagcgaa gaacacggca cagccttttc catctcttcc
     2881 tttggtggtg gcatactcca gagcgagcct ggtatggtca ggaagcgggt aggtaaaagt
     2941 ggctttttct ttccgggtaa aatcgccgtc tggcgga
//
LOCUS       DAOMAX010000311         2973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_6970_length_2973_cov_3.901988, whole genome shotgun sequence.
ACCESSION   DAOMAX010000311 DAOMAX010000000
VERSION     DAOMAX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_2973_cov_3.901988"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1478)
                     /locus_tag="PK742_12065"
     CDS             complement(99..1478)
                     /locus_tag="PK742_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQU77370.1"
                     /translation="MSFRTIIEPFRIKMVEPITMTTEEERKTLLREAHYNPFLLEAKD
                     VLIDLLTDSGTGAMSTRQWAGMVMGDESYAGAVSWQHMHAVIHRLTGMEHVLPTHQGR
                     AAERILYSYLGGPGKTFISNTHFDTTRANIEYSGAEALDIPIPEGTQPATIHPFKGNM
                     DTEKLEALLHEKKAENIGAVILTVTNNSGGGQPVSMANAREVSRLCRQHGVLFVLDCC
                     RIAENAWFINHREEGYGDMTYEAIAQEMFGLADAAIMSLKKDGLVNMGGFLTMRNKEL
                     SDACTNLLIITEGFATYGGLSGRDMEAIAIGLQEVFEPDYLRYRIRSTAYLGEKLAAM
                     GVPLIQPIGGHAVYVDAKAMLPNIPPSEYPGQALVCALYEVGGIRAVEIGSVMFGKYD
                     AAGQLVTAPMELVRLAIPRRVYTQSHMEYVIEVFEEVLRRKDQLKGLRIMEEPKFLRH
                     FTCKLERLH"
     gene            1581..1892
                     /locus_tag="PK742_12070"
     CDS             1581..1892
                     /locus_tag="PK742_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77371.1"
                     /translation="MDTTETAQRPDWSDMWEGLYYLSFFIFATLVVIPNLGKTLTFGY
                     DFRIAMIPYGTIGVFAGMGVYRLVRNAPVWLKMGIILLIYAAVMYQLITHVDRFTIPD
                     L"
     gene            2052..>2973
                     /locus_tag="PK742_12075"
     CDS             2052..>2973
                     /locus_tag="PK742_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQU77372.1"
                     /translation="MSETAKVLLDGQECELPVITGTENEKAIDISKLRDKTGYVTLDT
                     GYKNTGATTSAITFLDGEQGILRYRGYPIEQLAEKSNFLEVSYLLLYGELPSASQLTD
                     FTKRITRHTLVHEDVKKFFDGWPSSAHPMGQLCSLICSLSSFYPESLNPNRSQEEVDL
                     SIIRILAKMPTLVSWIYKKSIGHPLIYPNNNLDYVSNFLFMTYGMRTEDFHVDPVIVE
                     AMDKLLILHADHEQNCSTSTVRLVGSSQANPYASVAAGVAALWGPLHGGANQKVIEML
                     DQIQNDGGNTAKWIEKAKDKNDPFRLMGFGH"
BASE COUNT          677 a          753 c          743 g          800 t
ORIGIN      
        1 tggttcgtgg tccgctgtat ctgatattag ggatcctgac ctggtatctg caggtttatc
       61 ttggttataa gccagtcgtc caccttgact ggttctgatc agtgcagtct ttccagttta
      121 caggtgaaat gcctcagaaa ttttggttct tccatgatgc gcaatccttt tagctgatct
      181 tttctcctga gcacttcttc gaacacctcg atcacatatt ccatatgact ctgggtatag
      241 acacgccggg gtatggccag acgcaccagt tccatggggg cggtcacaag ctgaccggca
      301 gcatcatatt ttccgaacat gaccgagccg atctcgaccg cccggatgcc gccaacttca
      361 tacagagcac aaacaagagc ctgacctgga tattcacttg gtggtatatt cggtaacatg
      421 gcttttgcat ctacatagac tgcatgccca ccaatgggtt gaatgagcgg cacgcccatc
      481 gctgccagct tctcacccag gtaggcagtg cttcggatac ggtatcgcag ataatccggt
      541 tcaaagactt cctgcaatcc gatggcaatg gcttccatat ccctgccact cagaccgccg
      601 taggtggcaa aaccctccgt gatgatgagc aggttggtgc aggcatcgct gagttccttg
      661 ttacgcatgg tcaggaaccc gcccatattg accagtccgt cctttttcag actcatgatg
      721 gctgcgtctg ccaggccgaa catctcctga gcaatagctt cgtaggtcat atcaccatag
      781 ccttcctccc ggtgattgat gaaccaggca ttctcagcta tccggcagca atccaggaca
      841 aaaaggactc catgttgtct gcacagccgg ctcacctcgc gggcattggc catgctgacc
      901 ggttgcccgc cgccactgtt attggtcact gtcaggatca ctgctccaat attctctgct
      961 tttttttcat gcagcagggc ttccagtttt tctgtgtcca tattcccctt gaaagggtgg
     1021 atggtggcgg gttgcgtgcc ttccgggatg gggatatcca gagcttctgc gccactgtat
     1081 tcaatattgg ccctggtggt atcgaaatgg gtattggaga tgaatgtctt gccggggccg
     1141 cccagatagg aatagaggat cctttcagca gcacgaccct gatgggtggg cagtacgtgc
     1201 tccatgcctg tcaggcggtg gatgacagca tgcatgtgct gccagctcac agcacctgca
     1261 tagctttcgt cgcccatgac cattccggcc cattgccggg tactcatagc tccggtgcca
     1321 ctgtcggtca gcaggtcgat caagacatct ttagcctcca gaaggaaagg gttataatgt
     1381 gcttccctca gtagtgtttt ccgttcctct tcggtggtca tagtgatggg ttccaccatt
     1441 ttaatgcgga agggttctat aatggtcctg aatgacattt ggtctggttt agcagcgaag
     1501 gtagggcggt gttttacggc acccgatgat tatcatcacc ccggcatgtt attttctcct
     1561 gactgcgcta cctttgagtc atggatacca ctgaaacagc gcaaagaccc gactggtctg
     1621 atatgtggga agggctgtat tacctcagtt ttttcatttt tgccaccctg gtggtcatcc
     1681 cgaacctggg taagacgctg acctttggtt acgatttccg catcgccatg atcccttacg
     1741 gtactattgg ggtctttgcc ggaatgggtg tgtacagact ggtaagaaat gcaccggttt
     1801 ggttaaaaat ggggatcatt ctgctcattt atgcagcggt catgtaccag ctgatcaccc
     1861 atgtggatcg ttttaccatc cccgatctgt aaaagtgcta aactcaatga cacctggtgt
     1921 tgttacaggt tcagtaaacc gtgacagaga gtcttgcctt tctggccgaa aaatggaagc
     1981 atactcttcc tttcacggtg caaatgccct aactttgggg tggattttga atgatttaaa
     2041 ttttttaaaa tatgtctgaa acggcaaaag tactcctcga tggtcaggaa tgtgaacttc
     2101 cggttattac cggaacggag aacgaaaagg ccatagatat ttccaaactg cgtgataaga
     2161 ccggatatgt taccctggat acgggctaca agaatacagg tgctaccacg agtgccatca
     2221 cctttctgga tggtgagcag ggaattcttc gttacagggg atacccaatc gaacaactgg
     2281 ctgagaagag taattttctg gaggtttctt atctgttgct gtacggagaa ctgcccagtg
     2341 cgtcgcagtt aacagacttt accaagcgca tcaccagaca taccctggtg cacgaagatg
     2401 tcaaaaagtt cttcgatggc tggccttcat ctgctcaccc gatgggtcag ctctgttcac
     2461 tgatatgctc cctttcttct ttttatccgg aatcactgaa tccgaacagg agccaggaag
     2521 aagtggatct gagcatcatc cgcatcctgg ccaagatgcc aacactggtt tcctggatat
     2581 ataagaagtc catcggacat ccgctcattt atccgaacaa taacctggat tacgtcagca
     2641 acttcctgtt catgacctat ggtatgcgca cggaagactt tcatgtggat cctgtcattg
     2701 tagaggctat ggataaactc ctgatcctcc acgctgacca tgagcagaat tgttctacca
     2761 gtaccgttcg actggtgggt tcttctcagg caaatcctta tgcatctgta gctgctggtg
     2821 tggctgcact ctgggggccg ttgcacggtg gtgccaacca gaaggtcatt gaaatgctgg
     2881 atcagatcca gaacgatggc ggcaatactg ccaagtggat cgagaaggcc aaagacaaga
     2941 acgatccgtt ccgcctgatg ggctttggcc acc
//
LOCUS       DAOMAX010000312         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7022_length_2959_cov_4.176309, whole genome shotgun sequence.
ACCESSION   DAOMAX010000312 DAOMAX010000000
VERSION     DAOMAX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7022_length_2959_cov_4.176309"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PK742_12080"
     CDS             complement(<1..659)
                     /locus_tag="PK742_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77373.1"
                     /translation="MQVCFAQSAPARMAWYQPVDVPSNNISLSVIYDGEVSTLTGQQH
                     SYYLTWEDTIGTKKGYSQQNRPYELHVVVRDRDTIVGRYTGRSYPSTTCYFVTNAKQV
                     EVFFWLDINLWYEPPWTWKGEEHRAQEGPPLTTKEMLQEMSLVEKFWQRQEYAPIHIR
                     MDRSPMNIVDGYDHQQVILHPNPDIVGLDYINKGAMYPDNMPHYGPMYEQVMSLSRYQ
                     L"
     gene            complement(704..1939)
                     /locus_tag="PK742_12085"
     CDS             complement(704..1939)
                     /locus_tag="PK742_12085"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQU77374.1"
                     /translation="MLKFPGSYLLHDGKAFLSFLFLFSGAMDHLQAQNTVVEKSQAID
                     SVLQHYANEYSIVGLSVGVVHQNESYTFQYGYTDQSKRHPVTDSTMFLVSSITKLFTA
                     TAVFQLIEEGKLHTDDKITDLLPEFRMKDERYRNIRISHLLTHTSGLPWDVILKDSPD
                     DATALPLLLQELQRKKLSFTPGEALSYKTYSNVAFDLLGMVVESQTGMPIQEYVTARI
                     LQPLDMQRSTYFYETIDSTALAIPQVVSGNSRRVTRLNQYGRDSERNPVMNGKPLLVE
                     SYPVYGEEYEHNGSGNLISNAPELNLWMTELLRIYQDIAYDGVIGRSTLEDMWTIHQT
                     IAGKKTAIGWGWWTYPYEQWGTSVFHVGTNVGFCSILTIFPRHDLGITVLCNGWYAQE
                     AVWHGIADEVAKIFLDENQ"
     gene            complement(2149..>2959)
                     /locus_tag="PK742_12090"
     CDS             complement(2149..>2959)
                     /locus_tag="PK742_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQU77375.1"
                     /translation="YYEKEIDERQIKETFNFELVNKILNRNSVFTEKELEKLDQLQKV
                     YTANIIDLSPSEYQKELERLAIDLSWKSSQIEGNTYSLLETERLLKEKQTASGKTKEE
                     AVMLLNHKDALDFIIENQEYITPLTVSKIEDIHKMLTKELGVEKNVRKRRVGISGTNY
                     RPLDNEFQILEALKDACSVINNKESIFEKALLALVLISYIQPFMDGNKRTARIVSNAI
                     LMNYMYCPLSFRTVDAVDYKKAMLLFYEQNNISAFKDIFIGQFEFAVRTYF"
BASE COUNT          757 a          703 c          647 g          852 t
ORIGIN      
        1 tcgagctggt atctgctgag cgacatcacc tgttcataca tcggtccgta atgcggcatg
       61 ttatccggat acatcgctcc cttattgata tagtccagcc ccacaatgtc ggggtttgga
      121 tggagtatca cctgctgatg gtcatagcca tccacgatat tcattggcga acggtccatg
      181 cggatgtgga tgggagcata ttcctgtcgc tgccagaact tttccaccaa agacatctcc
      241 tgtaacatct cttttgttgt caaaggaggt ccttcctgag ccctgtgctc ctctcccttc
      301 caggtccacg gcggttcgta ccaaaggttg atatccaacc agaaaaaaac ttccacctgc
      361 ttcgcattgg taacgaagta gcaggtggta gatggatagc tgcgccctgt gtatcgccct
      421 acgatggtat ctcggtcacg caccaccaca tgcagttcat atggcctgtt ctgctgagaa
      481 taacctttct tcgtgccgat ggtatcttcc caggtcaggt agtagctgtg ctgctgtcct
      541 gtcagggtag aaacctcccc gtcatatatc acactcaagg agatattatt ggaaggcaca
      601 tccaccggct gataccaggc cattctggca ggagcgcttt gagcaaaaca tacctgcatc
      661 aggcaacatg ataccagata caagccgact gcgatcagat ttttcattgg ttttcgtcca
      721 ggaatatttt ggctacttca tcggctatgc catgccacac agcttcctgt gcataccagc
      781 cattgcaaag aacggtgatt ccgagatcat gccggggaaa aatggtaagg atgctgcaga
      841 atcctacatt ggtgcctaca tgaaaaaccg atgtacccca ttgctcgtag ggataggtcc
      901 accaccccca cccgatggct gtcttctttc cggcgatggt ctggtggatg gtccacatat
      961 cctccagggt actccggcct atcaccccat catatgcaat atcctgatat atacgcagca
     1021 gttcagtcat ccacaggttc aattcagggg cattggaaat gaggttgccg ctgccgttgt
     1081 gttcatactc ttctccatac acaggatagg attctaccag cagaggttta ccgttcataa
     1141 caggatttct ttcactgtct ctgccatatt gattcagtcg cgtcaccctt cgtgaattac
     1201 ccgaaacaac ctgaggtatg gccaatgctg tgctgtcaat cgtttcatag aaatatgtgc
     1261 tgcgctgcat atcaagaggc tgcaggattc gggcggtgac atactcctgg atcggcatgc
     1321 cggtctggct ttccaccacc atgcccagca gatcaaaggc aacattgctg taggtcttgt
     1381 aggataacgc ttctccgggt gtgaacgaga gtttcttcct ttgtaactcc tgcagcaata
     1441 aaggcaaagc agtggcatca tcgggcgaat ctttcaggat cacatcccag ggcagcccgg
     1501 aggtatgggt gagcagatgg ctgatgcgga tgttgcggta gcgctcatct ttcattcgaa
     1561 attccggcag cagatcagtg atcttatcgt ccgtgtgcag tttcccttct tctatcaact
     1621 gaaagacggc agtggctgta aagagtttgg tgatggacga aacgaggaac atggtactgt
     1681 ctgttaccgg atgccgcttg ctttggtccg tataaccgta ttgaaaagta tagctctcgt
     1741 tctggtgcac cacacccacc gacaatccga cgatggaata ttcatttgca taatgctgca
     1801 ggacggaatc aatggcctgg cttttttcaa caacagtgtt ctgtgcctgc agatgatcca
     1861 ttgcgccgga gaacagaaat aaaaaactta aaaaggcctt gccgtcatgc aataagtaag
     1921 atccaggaaa cttcagcact cgcagaagaa tggtcccgga gtggaatatg tgcaagaggc
     1981 aacgatcaaa atttcgggac atatactgat acggttctac agtttctgaa ataaaatata
     2041 ctcaaacaat aacagagcat cattcaatct ttttcacaat gccaacagag aaaaaaaata
     2101 caggccagaa ttcggggact tcagtagttg aatttcagtt gtctgatcct aaaaatatgt
     2161 tctgacagca aattcaaact gaccaataaa aatgtcttta aaggctgaaa tattgttctg
     2221 ttcatagaat agcagcatag ctttcttata atctactgcg tccactgttc tgaaagacag
     2281 cgggcaatac atgtaattca ttaaaatagc attgctgaca attcttgcag ttcgcttatt
     2341 gccgtccata aagggctgta tataggagat caacacgagg gcaagtagtg ctttttcaaa
     2401 gatgctttcc ttgttattta taacactgca agcatctttc agagcttcca ggatctgaaa
     2461 ctcattgtcc aatggtctgt aattagtacc tgaaatgcca acccgtcttt ttcttacatt
     2521 tttttcaacg ccaagttctt tggtcaacat tttatggatg tcctcaattt tcgaaactgt
     2581 aagcggtgta atatactcct ggttttctat gataaaatca agcgcatcct tatggtttaa
     2641 cagcatgacg gcttcttctt ttgtcttacc ggaggctgtt tgcttttctt tcaatagcct
     2701 ctcggtttcc agaagcgaat aggtgttgcc ctctatctgt gaagatttcc agcttaggtc
     2761 aatggccagt ctttccagtt ctttctggta ttctgatggg ctcagatcta taatattcgc
     2821 agtgtacacc ttttgaagct gatcgagttt ttccaattcc ttttccgtaa atacactgtt
     2881 tctgttcaag atcttattga ccagctcaaa attgaaagtt tccttgattt gtctttcatc
     2941 gatctctttt tcataatac
//
LOCUS       DAOMAX010000313         2954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7040_length_2954_cov_3.832701, whole genome shotgun sequence.
ACCESSION   DAOMAX010000313 DAOMAX010000000
VERSION     DAOMAX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7040_length_2954_cov_3.832701"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK742_12095"
     CDS             complement(<1..366)
                     /locus_tag="PK742_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77376.1"
                     /translation="MKKICLPVFLLVIPWFIRAQDTLDIRYDVNLETMAIIYNLSDAG
                     SFLFEHNPPPRAMLARAITNRFSDLSDHEAVQWVNYLYEHDLVDLYDIDLSLYMTPLP
                     EFRQYAPFPEMYYENDSLSA"
     gene            complement(495..941)
                     /locus_tag="PK742_12100"
     CDS             complement(495..941)
                     /locus_tag="PK742_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU77377.1"
                     /translation="MNIVIKDYLPSYKEEVLALFRMNVPDYFDQSEEAGLINYLDHEV
                     ESYFVVLIDDKLVGSGGINSEWEDGASVIAWDMIHPEYQGKGIGMQLLQHRISLLEQL
                     DPVPLIRVRTSQHTFGFYEKGGFVLQEVIPDYWAPGFDLYDMIYGS"
     gene            complement(938..1507)
                     /locus_tag="PK742_12105"
     CDS             complement(938..1507)
                     /locus_tag="PK742_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQU77378.1"
                     /translation="MATITAHMHVTLDGYAAGPNGEMDWILHSAELFESVHELTDRTG
                     VALYGRKTWMLMDQYWPTAADKPDASEHDKIHAAWYKTVPHHIISASRHGEVNGLDHF
                     WGEDVLGYVRRLKEESTDDILLLGSPSIQRLLMQEGLIDTARYYVNPVLLGGGIRVFP
                     ELTDRLVMRRKEVQTFGNGVVALEFDLQS"
     gene            complement(1508..2056)
                     /locus_tag="PK742_12110"
     CDS             complement(1508..2056)
                     /locus_tag="PK742_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HQU77379.1"
                     /translation="MTEQQHWNAVFIATSMDGFIAGKDGDISWLDTIPIPEGTDMGYH
                     AFMDRTDAILMGRNSFETVLGFGIDWPYAKPVFVWSRRSLTIPDELQEKVFPIQGRTS
                     EVLSQLHALGHRRLYIDGGMTIQSFMKEDLIDEMIITTIPVVLGEGISLFGELHPPTW
                     FRCVQSQIFEDAIVQSHYLKNT"
     gene            complement(2049..2627)
                     /locus_tag="PK742_12115"
     CDS             complement(2049..2627)
                     /locus_tag="PK742_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQU77380.1"
                     /translation="MNPKTNFFFEADGPWKEAYAEMRKIALGCGLTEELKWGCPCYTM
                     NKKNVVLIHGFKEYCAYLFFKGSLLLDPEGILIQQTENVQASRQIRFTSAAQIRKMKK
                     ELSAYIFEAMEAEKAGLKVALKKTKAYPVPDEFRSILTKDKKLKAAFEALTPGRQRGY
                     LLYFSQAKQAKTRMDRIEKYRNHILAGKGLYD"
     gene            complement(2677..>2954)
                     /locus_tag="PK742_12120"
     CDS             complement(2677..>2954)
                     /locus_tag="PK742_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77381.1"
                     /translation="HYGPMYEQVMSLSRYQLDMATGRIKKGQQWIRWKGIRKCCGMEF
                     RIVDPHLWYTDQNGKQYSGKALIRHRKLDPGLFSTDQFELLEDCLPY"
BASE COUNT          744 a          771 c          657 g          782 t
ORIGIN      
        1 ggccgacaga gagtcgttct cataatacat ctccggaaag ggagcatact gccgaaattc
       61 aggaagtggg gtcatataca gcgacaggtc tatgtcgtac agatctacca aatcatgctc
      121 gtacagatag ttcacccatt gcacagcctc atgatcagaa agatcgctga atctgttcgt
      181 gatcgccctg gccagcatgg ctcttggagg cgggttgtgt tcaaacagaa agctgcctgc
      241 atctgacagg ttgtagatga tggccatggt ttccaggttg acatcatagc ggatatccag
      301 ggtgtcttgt gccctgatga accagggtat tacaagcagg aaaaccggca ggcagatctt
      361 tttcatacag gcgtatataa caaaagtagt gcccgataac agaggcatac ccggaaaatg
      421 ataactgcag ggaatgacat gaaaagagtc tgcaatcaga attcagagaa cagcatccaa
      481 agggatggcc gccatcatga tccatagatc atatcataga gatcaaaacc cggtgcccag
      541 tagtcaggaa tgacctcttg caaaacaaat cctccttttt cataaaaacc aaatgtatgc
      601 tgagaggttc tgacccggat aagcggtacc ggatcgagct gctctagcaa actgatacga
      661 tgctgcagca gctgcatccc tatgcctttt ccctgatact ccggatggat catatcccag
      721 gcaataacag atgccccgtc ttcccactcg ctgttgatac cacctgaacc taccagcttg
      781 tcatctatga gtaccacgaa gtaagattct acctcatggt ccaggtagtt gatcagaccg
      841 gcttcttcag actgatcaaa gtaatcaggt acattcatgc gaaaaagggc caggacctct
      901 tctttataag aaggcaggta atccttgatc actatattca tgactgaaga tcaaattcaa
      961 gtgcaaccac accgtttccg aaggtttgaa cttcttttcg ccgcatcacc aggcgatcgg
     1021 tcaactcagg gaatacccgt atgcctcctc ccagcagtac cggattcaca taatagcgtg
     1081 cagtatctat caatccttcc tgcataagca atcgctggat ggaggggctg cccagcaaca
     1141 ggatgtcatc tgtgctttct tctttcaggc ggcgcacgta tcccaacaca tcctcacccc
     1201 agaaatgatc cagtccgttc acctctccat gtctggaggc agagatgata tgatggggta
     1261 cggtcttata ccaggcagca tgaatcttat catgttcaga tgcatcaggt ttatcagctg
     1321 cagtgggcca gtactgatcc atcagcatcc aggtctttct gccatataat gccaccccgg
     1381 tgcggtcggt cagttcatgt acagactcga acaattcagc gctgtgcagg atccagtcca
     1441 tttcgccgtt cggtccggcg gcgtaaccat ctaaagtaac atgcatatga gcggtgatcg
     1501 tggccatcta tgtgtttttt aaataatgtg actgtacaat tgcatcttca aagatctgtg
     1561 attggacaca tcggaaccag gtgggtggat gtaattcacc aaaaagggaa ataccctcgc
     1621 ccagcaccac cggaatggtc gtgatgatca tttcgtctat gagatcttcc ttcataaaag
     1681 actgtatggt catgcctccg tctatatata atcgacggtg acccaacgca tgcaattgcg
     1741 ataatacttc agatgtcctg ccctgaatgg gaaacacttt ttcctgcagc tcgtccggta
     1801 tggtcaatga tctacggctc cacacgaaca ccggtttggc atacggccag tcgatgccaa
     1861 aaccgagcac tgtttcaaag ctgttcctcc ccatgagaat ggcatctgtg cgatccatga
     1921 acgcatggta acccatatca gtaccttccg gtatcggtat ggtatcgagc caggaaatat
     1981 cgccgtcctt ccctgctata aaaccatcca tactggtggc tatgaagacc gcattccagt
     2041 gttgctgctc agtcatacag tccttttcct gccaggatat gattcctgta tttttctatc
     2101 ctgtccattc ttgttttggc ctgtttggcc tgggagaaat acaggagata ccctctctgt
     2161 ctgccgggag tcaatgcctc aaaagcggct ttcagttttt tatcctttgt caatatactt
     2221 ctgaactcat ccggcaccgg ataggctttg gtctttttca atgccacctt cagtccggct
     2281 ttctctgctt ccatcgcctc gaagatatag gctgaaagtt cctttttcat tttcctgatc
     2341 tgagctgcag aagtgaaacg gatctgcctg ctggcctgca cgttttctgt ctgctgtatg
     2401 agtatgcctt ccggatccag cagcaggcta cccttgaaga acagataagc acagtactcc
     2461 ttaaacccat ggatcagcac cacattcttt ttgttcatcg tatagcaggg gcaaccccat
     2521 ttcagctctt ccgtcaaacc gcagcccagt gcgatctttc gcatctcggc ataggcttct
     2581 ttccacggac cgtcggcttc aaaaaagaaa ttggttttag gattcattgc agcgtgtaaa
     2641 tctaccgaag attcaaggat ccgatcctgg tccggatcaa tacggcaaac aatcttccaa
     2701 cagctcaaac tgatcagtgc tgaacagtcc ggggtccagc ttcctgtggc gaataagtgc
     2761 ctttcctgag tattgctttc cgttctgatc tgtgtaccac agatgcggat ccacgatccg
     2821 gaattccatt ccgcagcatt tcctgatacc cttccatcga atccattgct gccctttttt
     2881 gatcctgccg gtggccatgt cgagctggta tctgctgagc gacatcacct gttcatacat
     2941 cggtccgtaa tgcg
//
LOCUS       DAOMAX010000314         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7094_length_2942_cov_3.513682, whole genome shotgun sequence.
ACCESSION   DAOMAX010000314 DAOMAX010000000
VERSION     DAOMAX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7094_length_2942_cov_3.513682"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PK742_12125"
     CDS             <1..1624
                     /locus_tag="PK742_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQU77382.1"
                     /translation="TSVVDQDGDTLEYSLGIAMEGRPYLGIPLSVEIRENTQKVSIGY
                     TTSPNAAALQWLTAEQTRDKKYPFLFTQSQAILARTWIPCQDGPGVRFTYEAQVEVPP
                     GMLALMSAENPQHISEDGSYHFKMEQRIPSYLMALAVGDIAFKPIGRNTGVYAEPSYV
                     DACAWELADMQDMLDQAEGLYGEYAWGRYDVIVLPPSFPFGGMENPRLTFATPTIIAG
                     DRSLTALVAHELAHSWSGNLVTNANWNDFWLNEGFTVYFENRIMEKVYGKDYADMLKV
                     LEYNDLSATVEEFGKDSPDTHLKLNLSDRDPDDGMSDIAYNKGAYFLRLLEQTVGREK
                     FDVFLHTYFQSHAFEVMDTETFVAYLQEHLIEPNKAAFEGIDINAWIYGPGIPDNCPV
                     VVSERFNLVDSALAAWKNGTPPAELNTKDWSTHEWLRFIKSLPDDITADQMTALDQAF
                     SFTGTGNCEVADVWYELALHRQYEPAYPAIDTFLCAVGRRKFLTPLYKAMIGTESGKA
                     MAMDIYKRARPTYHYVAVNTLDAMLGWDQEER"
     gene            1682..2695
                     /locus_tag="PK742_12130"
     CDS             1682..2695
                     /locus_tag="PK742_12130"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQU77383.1"
                     /translation="MQEAGIIFIVGNSRSGTTMMRRVLGNHPAIFMLEELHFFEQLWS
                     TADKEKVLTVPEAANLASRLMFIQRDGYLAEMNTGKHYEEAMNMVSSMESSLYPHEIL
                     RAFLHHETKKAGCSIPCEKTPQDVFYIKEILELFPEARIINMVRDPRGVMLSQKRKWQ
                     RRSMGAGFMTRKEQRRLRINYHPITISKLWNASQRAALPYTEHERVHTVIFEELVNDA
                     DKQVRDLCAFLGIAFQDSMLAIPQVGSSNEADKPDSFGIKTERAGNWEKGGLNKGEVW
                     FCQRICRAYMLSYGYSVKPVNPGVFYPVWYYISFPFKIMLALLFNLHRMKNIGEAIRR
                     RLR"
BASE COUNT          791 a          657 c          769 g          725 t
ORIGIN      
        1 tacctctgtg gtggatcagg atggtgacac gcttgaatac agtttaggca ttgcaatgga
       61 aggcagaccg tatctgggta ttccgctttc tgttgagatc agggagaata cacaaaaagt
      121 atctatagga tataccacat cgcccaacgc agctgcatta caatggctta cagcagagca
      181 gaccagagat aagaaatatc catttctgtt cacacagtcg caggccattt tggccagaac
      241 atggatcccc tgtcaggatg gacctggtgt gcgcttcacc tatgaggccc aggtagaggt
      301 tccgccgggc atgctggcgc tgatgagtgc agagaatccg cagcatatta gtgaagacgg
      361 ctcctatcat ttcaagatgg aacagcgtat ccccagttac ctgatggcac tggcggtggg
      421 cgatattgcc tttaagccga taggacgcaa taccggtgtg tatgcggagc cttcctatgt
      481 ggatgcctgt gcctgggaac ttgctgatat gcaggatatg ctggatcagg ctgaaggact
      541 ctatggcgaa tatgcctggg gcagatacga cgtgatcgtg cttccgccga gtttcccttt
      601 tggcgggatg gaaaatccaa gacttacttt cgctacaccg accatcattg ccggcgacag
      661 atcactgacc gcactggtgg cacacgaact ggcacacagc tggagtggta atctcgtaac
      721 gaatgccaac tggaacgact tctggctgaa tgaaggcttt accgtctatt tcgagaaccg
      781 catcatggaa aaggtatatg gcaaagatta tgcagatatg ctgaaggtgc tggagtacaa
      841 tgacctgtcg gctacggtag aagagtttgg aaaggattct cccgatacac acctgaaact
      901 aaacctgagt gatcgtgatc cggatgatgg catgagcgac attgcctata acaaaggagc
      961 atacttcctt cggttgctcg aacaaactgt aggccgtgaa aagtttgatg tgtttctcca
     1021 tacctatttt cagtcgcatg catttgaggt gatggacact gagacatttg tggcttattt
     1081 gcaggaacat ctgatcgaac cgaacaaagc agcatttgaa gggatcgata tcaacgcctg
     1141 gatctatggc ccgggaatac ctgataattg tccggtggtg gtttcagagc gtttcaacct
     1201 ggtggactca gctctggcag cctggaagaa cggaacacca ccggctgagc tgaacaccaa
     1261 agactggagc acgcacgaat ggctaagatt catcaagagt ctgccggatg atattacagc
     1321 tgaccagatg acagcactgg atcaggcttt ttcatttacc ggaaccggca actgcgaagt
     1381 agcagatgtc tggtatgaac ttgccctgca ccgtcagtac gaacctgcct acccggcaat
     1441 tgatactttc ctgtgtgcgg tgggaagaag aaaattcctg acgccattgt ataaggcgat
     1501 gatcggaaca gaaagtggta aggctatggc gatggatatt tataagcgtg ccaggcccac
     1561 ctatcattat gtagcagtca acacgctgga cgccatgctg ggatgggatc aggaggaaag
     1621 gtgatacagg acactctttt ccctctgtta acgagttacc tttagaacgc aaaaccaaaa
     1681 gatgcaggaa gctggtatca tcttcattgt cgggaattcc cggtccggca ccaccatgat
     1741 gcgacgggta ttgggtaacc atcctgccat attcatgctg gaagaattgc atttcttcga
     1801 gcagttgtgg tcaacggccg acaaagagaa agtcctgacc gtacccgaag cggccaacct
     1861 ggcatcacgt ctcatgttca ttcaacggga tggctatctg gccgagatga atacaggcaa
     1921 gcactatgaa gaggccatga acatggtgag cagcatggag tcatcattgt atccgcatga
     1981 aatacttcgt gcctttctgc atcacgaaac gaagaaagca ggttgctcga ttccatgcga
     2041 gaagacaccc caggatgtat tttatatcaa agagatcctg gaactgtttc cggaagcccg
     2101 tatcatcaat atggtgcgtg atccaagagg ggtgatgttg tcgcagaagc gcaaatggca
     2161 gcggcgttcc atgggggctg ggttcatgac cagaaaggaa cagcgcagac tgcgcatcaa
     2221 ttatcatccg atcacgatca gtaaattgtg gaatgcctca caacgtgcag cattgcctta
     2281 tacggaacat gaacgggtgc ataccgtcat ttttgaagag ctggtgaatg atgcggataa
     2341 gcaggtgcgt gatctttgcg catttctggg tattgcattt caggacagta tgctggccat
     2401 tcctcaggtg ggcagcagca atgaagcaga taagcccgac agttttggca tcaagaccga
     2461 gcgggcaggc aactgggaga aaggcggtct caacaagggg gaggtctggt tctgtcagcg
     2521 tatatgcaga gcatatatgt tatcgtatgg ttattctgta aagcccgtaa atccgggtgt
     2581 tttctatccg gtttggtact acatttcctt cccgttcaaa attatgttgg ccttgttatt
     2641 caatttgcat cgaatgaaga acatcggtga ggccatacgg cgcaggttgc gataagagat
     2701 agaattatat attctccaca ccctataccg gccacaaaca aatgctgttg ccagctattt
     2761 taattggagt ccgcagggta attgctaatc tgaatacatg aatcacttat tcacaatcaa
     2821 cttctgcaca aaagaacgag attgaccatc tgtaatttta atcagataca caccattttc
     2881 gaaatgatct atatgcaggg tgagtgcatt ggtgcctttc ggaattgtat gttcttcaac
     2941 ga
//
LOCUS       DAOMAX010000315         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7110_length_2937_cov_4.556211, whole genome shotgun sequence.
ACCESSION   DAOMAX010000315 DAOMAX010000000
VERSION     DAOMAX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7110_length_2937_cov_4.556211"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /gene="creD"
                     /locus_tag="PK742_12135"
     CDS             complement(<1..1280)
                     /gene="creD"
                     /locus_tag="PK742_12135"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HQU77384.1"
                     /translation="MENNENNGNLFQQISRWIRESVTLKLAVLVFLVLLLLIPSAWIQ
                     GVIDDRQSYSYEATEDITRKWSDEQTLTGPVLIIPFTRTYPVGNEPKTGEEVMRTAED
                     RLILLPEKMTANAALDPQTLKRGIFDVNVYSSEISMHASFSPPDWSRYDIDPQRIDWR
                     KAYLAVGISDMRGMYADPVCSLNKETYTAEAYSGLPEMGDSYSSPFANGIRIRLEDSA
                     ARAAFDCDIQIGLKGSRSFFMLASGQNSSIEMTGNWPDPSFSGYFLPDDRDVTDSGFT
                     ASWNILKFTRPLPEAWISNIPDIENTGVGVELMSAVNDYQRTSRTSKYGALIIIFTFL
                     GLFLTELISRIRVHPFQYVLIGVALVIDYTLLLSISEQLGFNTAYLISSLATTALIAL
                     YTRSFSSWRVTGLLAALLVIFYTFIYIIINQQDYA"
     gene            complement(1329..1628)
                     /locus_tag="PK742_12140"
     CDS             complement(1329..1628)
                     /locus_tag="PK742_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQU77385.1"
                     /translation="MRFSLDQYDKAFESKLRLQIMSILMVHRQFDFNAFKELLDVTDG
                     NLASHMRYLEKTGYVESHKQFVGRKPNTSYTVTPAGKKAFTKHLEALENLLKNNN"
     gene            complement(1625..2239)
                     /locus_tag="PK742_12145"
     CDS             complement(1625..2239)
                     /locus_tag="PK742_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77386.1"
                     /translation="MDVHQANDDLRSIREIMERSVRFISLSGWAGVLAGSYALAGAAY
                     AYNRLYLPGSFHLRSELISMSTLWELLFAAVIVLVLSVSTAILLTVRKAREQGVRYWN
                     QAARDLLYQAGTPLVAGGLLTIALVWRGYWAVAAPSTLVFYGLALFSASRITWKEVRT
                     LGLLEIILGLTGVMLPGYGLFFWAAGFGLLHILYGFFMYRKYER"
     gene            complement(2296..>2937)
                     /locus_tag="PK742_12150"
     CDS             complement(2296..>2937)
                     /locus_tag="PK742_12150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQU77387.1"
                     /translation="VIPERGKHPVPSLQEQLSAIHGKLWIVHRIDRDTSGLVVIAKTA
                     EAHAHLSQQFEMHSIRKEYLALVYGVPAESPAQIDVPLKPHPTKNIMLVDGSGKPSTT
                     LIKTEEAFRRHTLLRVRILTGRRHQIRVHLAYIGNPLLVDTVYGSADAFYLSAVKKGY
                     KSVGEERPLISRLTLHATLLEFIHPASEEPVLIESPLPADMEVVCKQLRRWDS"
BASE COUNT          717 a          754 c          746 g          720 t
ORIGIN      
        1 gcatagtcct gctgattgat gatgatgtag atgaatgtgt aaaatatgac cagtaaggcg
       61 gccagcagac cggtcacccg ccagctactg aaactcctgg tgtacagggc tatcagtgcc
      121 gtggttgcca gactgctgat gagataggca gtattgaaac ccaattgctc cgatatcgac
      181 aacagcaatg tgtagtcaat gaccagtgcc acgcctatca gtacatactg gaatggatga
      241 acccgaatcc gggaaataag ctcggtcaga aacaatccta agaatgtgaa tatgatgatg
      301 agtgctccgt acttggatgt gcgactggta cgctggtaat cattgaccgc cgacatcagc
      361 tccacaccca ctcccgtatt ctctatgtca ggtatgttgc tgatccaggc ttcaggaagt
      421 ggtcgggtga acttcaggat gttccatgat gctgtgaatc cgctgtcggt cacatccctg
      481 tcgtcgggca ggaaataacc ggaaaaggaa ggatccggcc agttgccggt catctcaatg
      541 ctgctgttct gtccactggc cagcataaag aacgaacggc tgccttttag cccgatctgt
      601 atatcgcagt caaatgctgc acgagccgca ctgtcttcca gtctgatccg gatgccattg
      661 gcaaaaggac tggaataact gtcgcccatt tccggcagac cgctgtatgc ttctgccgtg
      721 tacgtctcct tattcaggct gcaaacggga tcagcataca taccccgcat gtctgatatg
      781 ccaactgcca gataagcttt gcgccagtcg atccgctgcg gatcaatgtc atacctggac
      841 cagtcgggcg gactgaagct ggcatgcata ctgatctctg aagaatacac attcacatca
      901 aagatgcccc gcttcagggt ctgcgggtcc agtgcagcgt tcgcggtcat tttttccggc
      961 aacagaatga ggcggtcttc ggcagtgcgc atgacctctt ctccggtctt gggttcatta
     1021 cctaccggat aggttctggt aaatggaatg atcagtacgg gtccggtcag cgtctgttca
     1081 tcagaccatt tgcgggtgat gtcttctgtt gcctcatatg aataggactg ccggtcgtcg
     1141 atcacgccct gtatccatgc gctcggaatg agcagcagca ataccaggaa caccagtact
     1201 gccagtttca gtgtaacgga ttcgcggatc catctgctga tctgctgaaa gaggttacca
     1261 ttgttttcgt tgttctccat gatgtgtagt tttactttgt actacaaagt gaatgatcaa
     1321 aaaaaatatc agttgttgtt cttcaggagg ttttccagtg cctccaggtg tttggtgaat
     1381 gctttttttc cggcgggtgt gaccgtgtag ctggtattgg gtttccgccc tacgaactgt
     1441 ttatgtgatt ccacataacc tgtcttttcc agatacctca tatgcgaggc caggttgccg
     1501 tcggtcacgt ccagcaactc tttgaacgca ttgaaatcga actgccggtg caccatcagg
     1561 atagacatga tctgcaggcg cagtttactt tcaaaagctt tgtcgtattg gtccagcgaa
     1621 aatctcaccg ttcatattta cggtacatga aaaatccata caggatatgc agcaaaccaa
     1681 aaccggcagc ccagaagaac aggccgtaac cgggtaacat cactccggtc aagcccagta
     1741 tgatctccag cagaccaagt gtgcgcacct ccttccaggt gatgcgggaa gcgctgaaca
     1801 gggccagccc gtaaaaaaca agggttgatg gagcggctac tgcccagtaa ccccgccata
     1861 ccagggcgat ggtaagcaac ccacctgcta ccaggggagt tcctgcctga tacaacagat
     1921 cgcgggcagc ctgattccag tacctgacac cctgctctct ggccttgcgc acggtgagca
     1981 ggatggcagt agaaacagaa agcaccaata cgatcacggc tgcaaacagc aattcccata
     2041 aggtggacat actgatcagc tctgatctga gatggaagct gccaggaaga tagagccggt
     2101 tataagcata agctgcaccc gccagtgcat agctgcctgc cagcacacct gcccagccgc
     2161 tgagtgaaat gaaacgcaca gagcgctcca tgatctcgcg aatggaacgc aggtcgtcgt
     2221 tggcttgatg tacatccata gaaagtactt tgtactacaa agttatttct gaactttcat
     2281 atctccaaat ctttttcagg aatcccagcg acgaagttgt ttgcagacca cttccatatc
     2341 tgccggtaag ggtgattcta tcagcaccgg ctcttctgag gccgggtgta tgaactccag
     2401 cagggtggcg tgcagggtga ggcggctgat gagcggtcgt tcttctccaa cggatttata
     2461 ccctttcttt actgctgata aataaaaagc atcagctgaa ccgtacaccg tatcaaccag
     2521 aagaggattt ccaatgtaag ccaggtggac cctgatctga tgccgccggc cggtcaggat
     2581 gcgcacgcga agtaaagtgt gtcttctgaa tgcttcttct gttttgatga gggttgtgct
     2641 tggcttaccg cttccgtcca ccagcatgat attcttagtg ggatgaggct tcagcggtac
     2701 atcaatctgc gcaggagatt cggccggaac gccataaaca agagccaggt attccttgcg
     2761 aatgctgtgc atctcaaatt gctgcgacag atgcgcatgt gcttccgcag ttttggctat
     2821 caccacgagt ccactggtat cacggtcgat ccggtgaacg atccacaact ttccatgtat
     2881 agcagacagt tgctcctgca aggatggaac aggatgcttc ccccgctccg gaatgac
//
LOCUS       DAOMAX010000316         2930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7149_length_2930_cov_3.505043, whole genome shotgun sequence.
ACCESSION   DAOMAX010000316 DAOMAX010000000
VERSION     DAOMAX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7149_length_2930_cov_3.505043"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PK742_12155"
     CDS             <1..553
                     /locus_tag="PK742_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HQU77388.1"
                     /translation="SKWISTVRLEMDGTQRLWRIRSDGRKARPLYPEFHDIGYYGAMP
                     AGSYAAFVLPEPFTLQYWNRASLLPETVDSSVGRSLKPYPNSNELFYVSKKDSTSWTI
                     NKWKPGELPAAVTSIPPDMEDMAWSTDGNLFMAKGSVIYYFDYMESGNWYEVADLSVF
                     GLKNIFRLAFSPDGNWVAFVAEE"
     gene            561..1397
                     /locus_tag="PK742_12160"
     CDS             561..1397
                     /locus_tag="PK742_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQU77389.1"
                     /translation="MNTILFATDFSEASEHAFRQVLPVADQLRAKIYFVHVEHVPATD
                     IQASGGMLEQVMQHRRQAAMEGLNKMKWETAEFAFNNNTHIESDHILTTGMIVEEIIH
                     AADSIQPQFVVLGVPSHHHFPALFGGSVAGTLIRKCSWPLLLFPPVITRYPAGDILFA
                     SGWDAGDEEILSRLMTLAAQLRKKVQLLHISQARENGSEQQVEAYIRKHFSKELESGH
                     LRSVVLYEDDITGAVLSYMEANEISLLVAGHHKHMIEAFHRQIAVKLSDHSHTPVLIY
                     QS"
     gene            complement(1388..>2930)
                     /locus_tag="PK742_12165"
     CDS             complement(1388..>2930)
                     /locus_tag="PK742_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77390.1"
                     /translation="PSPTGDPDPRGCVFGVTAGGPFAYHLLPSTEVKGLMYNNTDQSH
                     TDMTLQAVYAVYEHEFGEHYSGTEMELIIADAFGYPKTLAMLKGLSATFNSKWEDKGS
                     RYWALCLYQAGAAPVLHDILDADSYQQRSPLIMQACASLFTQYLLATYTPAEVRSLYN
                     SATSEELMQEAEDYDSWLRKQLQTFEPEKQKKKSLERLQGFNFAHEGYNVYNGYLGSE
                     ARKSIDEMHNTGSNTMAIIPYSVTREMNKPMPFPIMQSAGSENDASVIKAAHEAQERG
                     MVVMLKPQIWSHMGWPGDIAMKNEEDWSLFFSYYENWIMHYALLAEMYDIELFCAGVE
                     FQQATLTHPEAWETLFRKIRSLYGGYLTYAANWGAEIEGARIWDQLDFISVNCYYPIS
                     KQESPTDEELLSGMEAVLDKLEQIDRRTDKPMMITEIGFKSIDKPWIQPHADHDEQGV
                     NNDSQVRCYEAMFRALKDESWIQGIYLWQWPSYMDYYRHNPKGFTPAGKPAEEVVRKY
                     FTNQD"
BASE COUNT          734 a          696 c          676 g          824 t
ORIGIN      
        1 cagtaaatgg atcagtactg tgcggctaga aatggacggc acccagcgtt tatggcgtat
       61 ccgttcagac ggcagaaaag cccgtcctct ctatcctgaa tttcatgata taggttatta
      121 cggagccatg cctgcaggtt cttatgcagc cttcgttttg cctgagcctt ttaccctgca
      181 atactggaac cgggcttctt tacttccgga aacggttgac tcatcagttg gcagaagcct
      241 gaagccttac cccaatagta atgaactgtt ctacgtatct aaaaaagaca gtaccagttg
      301 gacgatcaat aaatggaaac ccggtgagtt gccggccgca gtaacttcca tcccacctga
      361 tatggaagac atggcctgga gtactgacgg aaatctgttc atggccaaag gatcagtgat
      421 atattacttt gattatatgg aaagcggtaa ttggtatgaa gtagccgacc tgtctgtttt
      481 tggattgaag aatattttca gacttgcctt tagtcccgat ggaaactggg tggcttttgt
      541 tgctgaagaa taaacaatgt atgaatacta ttttatttgc taccgacttt tctgaagcct
      601 ctgagcacgc attcagacag gtattgcccg ttgctgatca actcagggcg aagatctatt
      661 ttgtgcatgt ggaacatgtg cctgcaactg atatacaagc cagcggcggt atgctggaac
      721 aggttatgca gcatcgccgc caggcagcta tggaaggact caacaaaatg aaatgggaaa
      781 ctgccgaatt tgcctttaat aacaacaccc acatagaatc tgatcatatt cttactacag
      841 gtatgattgt agaagagatc atacatgctg ccgatagcat ccagccgcaa tttgtggtac
      901 tgggtgtacc ttcacaccac cattttccag ctctttttgg aggttcagta gccggtacat
      961 tgatcagaaa atgctcctgg ccgttgttgc tttttcctcc tgtgataacc agataccctg
     1021 ccggtgatat cctgtttgct tcaggctggg atgccggaga tgaagagatc ctgtccaggc
     1081 tcatgacgct ggcagcacaa ctgagaaaga aggtgcaatt actgcatatc agccaggcca
     1141 gagagaacgg aagcgaacag caggtagaag cctatatcag aaaacatttc agcaaggaat
     1201 tagaatcagg tcatctgaga tctgttgtgc tttatgaaga tgacattacc ggtgcggtct
     1261 tgtcatatat ggaagccaat gagatcagtt tgctggtagc aggacatcat aaacacatga
     1321 tagaagcatt tcacagacag atcgcagtaa agctttctga ccattctcat actccggtac
     1381 tgatctatca gtcctgatta gtgaagtatt ttctgacaac ctcttctgcc ggtttgcccg
     1441 ccggagtgaa tccttttggg ttatgccgat agtagtccat ataactgggc cactgccaga
     1501 ggtagatacc ctggatccat gattcatcct tcaacgccct gaacattgcc tcatagcacc
     1561 tgacctgcga atcattattt acaccctgct catcatgatc agcatgaggc tgtatccagg
     1621 gcttatcaat gctcttaaag ccgatctctg tgatcatcat aggtttgtct gttcgccggt
     1681 ctatctgctc cagtttatcc agtacggctt ccatgcctga aagcaattct tcatctgttg
     1741 gactttcctg tttactgatc gggtagtagc agttcacaga tatgaaatcc agctgatccc
     1801 atatccgggc gccttctatc tcggcacccc agtttgccgc ataggtcaga tatcccccgt
     1861 agaggcttct gatcttcctg aataaggttt cccatgcttc cggatgggtc agggtagctt
     1921 gttgaaattc tactcctgca caaaacagtt caatatcata catctccgcc agcaatgcat
     1981 agtgcatgat ccagttttca taatatgaaa aaaagagaga ccagtcttct tcattcttca
     2041 tggcaatatc gcctggccac cccatatgac tccatatctg cggtttgagc atgaccacca
     2101 tgccccgttc ctgcgcttca tgtgctgctt tgatgacact tgcatcgttt tcgctgccgg
     2161 cagactgcat gatagggaag ggcataggct tgttcatttc cctggtcacg gaatagggaa
     2221 tgatggccat ggtgttactg cctgtattgt gcatttcatc aatagacttc cgggcttcac
     2281 tgcccaggta tccattgtac acattatatc cttcatgggc aaagttgaat ccctgaagcc
     2341 tttccagaga tttctttttt tgtttttcag gttcaaaggt ctgaagttgt ttccttagcc
     2401 aggaatcata atcttctgct tcttgcatca gctcttcaga tgtggcgctg ttgtacaaac
     2461 tcctgacttc ggcgggggta tatgtagcca gcagatactg tgtgaacaaa gaagcacagg
     2521 cctgcataat gagcggcgac cgctgttgat agctgtcagc atcgaggatg tcatgcaata
     2581 ccggtgctgc gcctgcctgg taaaggcaaa gtgcccagta cctgcttccc ttatcttccc
     2641 atttactgtt gaacgtggca gacagaccct ttagcatggc caatgttttt ggataaccaa
     2701 aagcgtctgc aatgatcagt tccatttctg taccgctgta atgttctccg aattcgtgtt
     2761 cataaaccgc ataaacagcc tggagagtca tatctgtatg gctttggtcg gtattgttgt
     2821 acatcagacc tttgacctct gtgctgggta acaggtggta ggcaaaaggc cctccggcag
     2881 taactccgaa aacacaccca cgggggtcag ggtcaccagt cggcgatggg
//
LOCUS       DAOMAX010000317         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7159_length_2927_cov_3.528900, whole genome shotgun sequence.
ACCESSION   DAOMAX010000317 DAOMAX010000000
VERSION     DAOMAX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_2927_cov_3.528900"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK742_12170"
     CDS             <1..956
                     /locus_tag="PK742_12170"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQU77391.1"
                     /translation="IEEYGLAQVSMNLTNISITPVHIAFDECVKSAYNRGMRVTGSEL
                     VGLIPLQAMLDAGKYFLEKQQRSTGVSEEELIHIAIKSMGLDELTPFDPKEKIIEYKL
                     RGGANTGRLVGMNLRTFANETASESVAPGGGSISAYAGALGISLATMVANLSSHKRGW
                     DERWKEFSDWAEKGQRLKDELLHLVDEDTEAFNRIMQAFGMPKGSPEEKAARSAAIQE
                     ATKYAIEIPFRVMQLSLESMHVAKAMAETGNPNSVTDAGVGGLCARTAVIGAHMNVLT
                     NAAGLKDKDFLDRILPEAQRMTNEAEALEQEIRAIVIRNIG"
     gene            complement(953..2122)
                     /locus_tag="PK742_12175"
     CDS             complement(953..2122)
                     /locus_tag="PK742_12175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HQU77392.1"
                     /translation="MGRFDTLAGRVLQAYKNYNGKEPLYLWLKEYYRQNKQMGSTDRK
                     LFSALFFGLFRFKGGRPVRPDEDMINMAACLQWPLPAAYGNYLTEKDERYRKVMDLAE
                     AHQWAEAAQLAGWEEEGYFPMAGSVSDAIDRIQLELAHLHPSHVWIRVRSGFEKEVGE
                     ELRAAGYEWEEIQPRCWRMKGHYPLHTSKTWEAGKFELQDIASQETATGMHPESGQAW
                     FDCCAGAGGKSLMLLDKCADISLTVNDTRPDMLDELQRRLVKAGLPLPKTMQADLSLG
                     IYEGLGPFDGIIADLPCTGSGTWGRAPERLHFFREEEIAKYAALQLDITSQAYKVLKP
                     EGKLFYITCSVFREENEENIDRITANLPLKCTSQTYHFHHPDGGDTLFVAELEKA"
     gene            complement(2124..2342)
                     /locus_tag="PK742_12180"
     CDS             complement(2124..2342)
                     /locus_tag="PK742_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HQU77393.1"
                     /translation="MEKTYTNGEVTIVWKPDLCQHAGNCVRGLPGVFNNKARPWIDPQ
                     GADTDAIITQVQKCPSGALSFRMNKEEK"
     gene            2406..>2927
                     /locus_tag="PK742_12185"
     CDS             2406..>2927
                     /locus_tag="PK742_12185"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HQU77394.1"
                     /translation="MERITESTSHYDHLENMSTAELLRHINQEDRSVPQAIEQVLPAI
                     EKLVDVVADRMLAGGRLFYIGAGTSGRLGIVDASECPPTFGVPHGLVIGIIAGGDAAI
                     RKAVEHAEDDVDGSWRDLQAYDIHEQDVVVGIAASGTTPYVVGALQHCREHGITTGCI
                     TCNPGSPLAAAADH"
BASE COUNT          703 a          757 c          781 g          686 t
ORIGIN      
        1 atatagaaga atacggactg gcgcaggtga gcatgaacct gacaaatatc agcatcacac
       61 cggtacatat cgcatttgac gaatgtgtca aaagtgcata taacagaggt atgcgggtaa
      121 ccggcagcga gctggtgggc ctgattccgc tgcaagccat gctggatgcc ggaaaatatt
      181 ttctggagaa acagcagcgt tccaccggtg taagcgaaga agaactgatc cacattgcca
      241 tcaagagcat ggggctcgat gaacttacgc ctttcgatcc gaaagaaaag atcattgaat
      301 acaaactgcg tggcggtgca aataccggcc gactggtcgg catgaacctg cgcacctttg
      361 ccaacgagac cgccagtgaa tcagtagcac cgggtggtgg ttccatttct gcttatgcag
      421 gtgccctggg tatctcactc gctacgatgg ttgccaacct ttcgtcgcac aaacgtggat
      481 gggatgagcg ctggaaggaa ttttcagact gggctgaaaa agggcaaagg ctgaaagatg
      541 agttgcttca cctggtggat gaagacaccg aagcatttaa ccgcatcatg caggcattcg
      601 ggatgccgaa aggaagtccg gaagaaaagg cagcaagatc ggccgctata caggaagcca
      661 cgaaatatgc cattgaaatt ccttttaggg tgatgcaatt gtcgctggag agcatgcatg
      721 tggccaaagc catggcagag accggcaatc ccaatagtgt gacagatgca ggtgtgggcg
      781 gactatgcgc cagaactgct gtgatcggtg cgcatatgaa tgtgctgacc aacgcagccg
      841 gactgaagga caaggatttc ttagacagga tactccctga agcacagcgc atgaccaatg
      901 aggccgaggc actggaacag gagatccgtg ccatcgtcat caggaatatc ggctaggctt
      961 tttccagttc tgcgacgaac aaggtatcgc ctccgtccgg gtgatggaaa tgataggtct
     1021 ggcttgtaca cttcagtgga aggttcgccg tgatgcgatc gatgttctct tcgttctctt
     1081 cgcggaacac agaacaggtg atgtaaaata actttccttc cggcttcaat actttatatg
     1141 cctggcttgt gatgtccagc tgcagtgccg catatttggc gatctcttcc tcccggaaga
     1201 aatgcagccg ttcgggagct cttccccagg taccggaacc ggtgcagggc aggtcggcga
     1261 tgatgccgtc aaaggggccg agaccttcat agataccaag gctcagatca gcctgcatgg
     1321 tcttcggtaa tggtaatccc gctttcacca gtcttctttg taactcatcc agcatgtcgg
     1381 ggcgggtatc gttgaccgtc agactgatat cggcacactt gtcgagcagc atgagagact
     1441 tcccccctgc acctgcacaa caatcaaacc atgcctgtcc gctttccgga tgcatccctg
     1501 tggctgtttc ctggctcgca atatcctgga gctcaaactt acctgcttcc caggtcttcg
     1561 aagtatgtaa gggatagtgt cctttcatcc gccagcaccg gggctgtatc tcctcccatt
     1621 cgtatcccgc agcccgcagc tcctctccta cttctttttc aaatccggag cgtaccctga
     1681 tccagacatg agatggatgc agatgtgcaa gctccagctg tatccggtca atggcatcac
     1741 tgaccgaacc ggccatgggg aagtagcctt cctcctccca tccggcaagt tgtgctgctt
     1801 ccgcccactg gtgcgcttca gccagatcca tcaccttcct gtatcgttca tctttctctg
     1861 tcaggtaatt accgtaggct gcaggcaaag gccattgcag gcaggcagcc atattgatca
     1921 tatcttcatc cggcctgaca ggtctgcctc ctttgaatct gaacaagcca aaaaacaagg
     1981 cagaaaagag ttttctgtca gtggaaccca tctgtttgtt ctggcgataa tactccttca
     2041 accacagata caggggttct ttcccgttgt aattcttgta ggcctgcagc acacgccctg
     2101 ccagtgtatc aaatctgccc atgttatttc tcctccttgt tcatgcgaaa gctcagtgca
     2161 ccactagggc atttttgcac ctgagtaatg atggcatcgg tgtcggctcc ctgcggatct
     2221 atccatgggc gggccttgtt gttgaacacc ccgggcaatc cgcgaacgca gttacctgca
     2281 tgctgacaga gatcgggttt ccagacgatg gtcacctcgc cgttggtata ggtcttttcc
     2341 atttgagtgt atttgcccgc agcaagtata ggggcaatct gcgaggttgc ctaattttaa
     2401 ccgatatgga acgcatcacg gagagcacat cacattatga tcatctggaa aacatgtcaa
     2461 ctgcagagct gctgcggcat atcaaccagg aagacagatc ggtaccgcaa gccatagaac
     2521 aggtgctgcc agccattgaa aaactggtgg acgtggttgc cgacaggatg ctggcaggtg
     2581 gacggttatt ctacatcggt gcgggcacat ccggcagact gggcattgtg gatgcttctg
     2641 aatgtccgcc cactttcggt gtaccgcatg gactggtgat cggcatcata gcaggcggag
     2701 atgctgccat cagaaaagcg gtggaacacg ccgaagatga tgtggacggt agctggcgtg
     2761 atcttcaggc ttatgacatt catgaacagg atgtagtggt agggatcgca gcatccggca
     2821 ccacgcccta tgtggtggga gcattgcagc attgcaggga acatggtatt accacaggct
     2881 gcatcacctg caatcccggt tcaccactgg cagctgcagc agatcat
//
LOCUS       DAOMAX010000318         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7168_length_2925_cov_4.103136, whole genome shotgun sequence.
ACCESSION   DAOMAX010000318 DAOMAX010000000
VERSION     DAOMAX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7168_length_2925_cov_4.103136"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PK742_12190"
     CDS             complement(<1..198)
                     /locus_tag="PK742_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQU77395.1"
                     /translation="MSPTQLLHRWVELFNQADADTLADLYHEDAVNFQVNFKPIEGRE
                     AIRQKFREEFATAEMVCIIEQI"
     gene            complement(371..1009)
                     /locus_tag="PK742_12195"
     CDS             complement(371..1009)
                     /locus_tag="PK742_12195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQU77396.1"
                     /translation="MKRFTLMLTGLLLFAGSAFAQLSKAEMKELPDGLYAQMQTSKGN
                     ILLFLEFQKTPLTVANFVGLAEGTIANDAKPAGTPYYDGIVFHRVIADFMIQGGDPTG
                     TGRGGPGYRFKDEFHSDLKHTSAGILSMANAGPFTNGSQFFITHKDTPWLDGKHAIFG
                     HVVSGQEVVDAIAQGDTINHIMIIRKGKMAKKFDAAAVFAAAKEAGDVKADK"
     gene            complement(1025..2032)
                     /locus_tag="PK742_12200"
     CDS             complement(1025..2032)
                     /locus_tag="PK742_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQU77397.1"
                     /translation="MRLEKNLPELFEFLSTPRNIALVAHTRPDGDAIGSCLALKFFLE
                     KRGHTAEVIAPDDFPDFLNWMKGSDKVFIRQKNPKTCFAILANSDLIGFLDFNALKRI
                     ESLGEDIAKIRGKKWLMVDHHRDPDDFTDFTFWSDEASSTCELVYDLIRMMDGDADID
                     TDMAAIIYAGMAMDTGVFQYSNTTARVHEIAANLMHKGIHVEEIHNNVYNQYGENRLR
                     FIGYMLKDKMQILPEYRTAFMTISMKEAEEYKLSSGDKEGIVNLPLAMKDVDIAVLFT
                     EDKDRIKISFRSKGDINVDRLAREYFNGGGHKNASGGSTRVSLEETVQILHTALAAFL
                     S"
     gene            2490..2909
                     /locus_tag="PK742_12205"
     CDS             2490..2909
                     /locus_tag="PK742_12205"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQU77398.1"
                     /translation="MAGNITLTMIKPDAVKNNHIGGILKMINEAGFRIQAMKYTRMTR
                     EVAGAFYAVHKERPFYGELVDFMSSGPIVAAVLVKDNAVEDFRKLIGATNPAEAADGT
                     IRKMYATSIGENAVHGSDSDENAAIEAGFHFSAYERF"
BASE COUNT          712 a          707 c          766 g          740 t
ORIGIN      
        1 gatctgctcg atgatgcaga ccatctctgc ggtggcgaat tcttccctga atttctgacg
       61 gatggcttcc ctgccttcaa taggtttgaa gttcacctgg aagttgacgg catcttcatg
      121 gtagagatct gccagggtgt ctgcatctgc ctggttgaat aactcaaccc atcggtggag
      181 taactgcgtg ggcgacatgc tataaaatta agcaagcaga acaaatgaat gaacacggtg
      241 ctgacgtttg tcagcacaca ggtcacggca gatcccgtga caaacacaga ggacacggag
      301 gtctgctccg aaccattcac aaacaaaaag accggcacga tagccggtct ttccttgata
      361 ttttttctga tcacttgtca gccttcacat cgccggcttc tttagctgct gcaaacactg
      421 ctgctgcgtc gaatttcttt gccatttttc ctttgcggat gatcatgata tggttgatgg
      481 tatctccctg tgcgatggca tccaccactt cctgcccgct caccacatga ccaaagatgg
      541 cgtgttttcc atccaaccag ggggtatcct tgtgggtgat gaagaactgg ctgccattgg
      601 tgaagggacc tgcattggcc atactcagaa tgcccgcact cgtgtgtttc aggtcagaat
      661 gaaattcatc tttaaaacga tagccgggac caccgcgacc ggtaccggtt gggtcaccgc
      721 cctggatcat gaaatctgca atgacacgat ggaaaacaat accatcataa tatggtgtac
      781 ctgcaggctt tgcatcattg gcgatggtgc cttctgcaag gccaacgaaa ttggctacag
      841 ttagcggggt tttctggaat tccaggaaca ggaggatgtt gcctttggag gtttgcatct
      901 gggcatagag gccatcaggc agttccttca tctctgcttt gctcagttgt gcgaaagctg
      961 agccggcgaa aagcagcaga ccggtcagca tcaaagtaaa tcgcttcatt gttggatatg
     1021 ttgtttatga tagaaatgca gctaatgctg tatgcaaaat ctgtaccgtt tcttccagac
     1081 tgaccctggt ggagccaccg gaggcgttct tgtgtccgcc tccattgaaa tattctctcg
     1141 ccaggcggtc cacattgata tctcccttac tccggaaaga tattttgatg cggtccttgt
     1201 cttcggtgaa caacacggca atgtccacat ctttcatggc caagggaagg ttgacgatgc
     1261 cttccttatc acctgagctg agtttgtatt cttccgcttc tttcatggaa atggtcatga
     1321 atgcggtgcg gtactcaggc agtatctgca ttttgtcttt tagcatatag ccgatgaatc
     1381 tcagcctgtt ctcgccgtac tgattataga cgttgttgtg gatctcttcc acatgtatgc
     1441 ctttgtgcat caggttggcc gcgatctcat ggacccgggc ggtggtattg ctgtactgga
     1501 acacgccggt atccatcgcc ataccggcat aaatgatggc cgccatatcg gtgtcaatgt
     1561 cggcatcacc atccatcatc cggatgaggt catacaccag ttcgcaggtg gagctggctt
     1621 cgtctgacca gaaggtaaaa tcggtgaaat catccgggtc gcggtggtga tccaccatca
     1681 gccatttctt accacggatc ttcgcaatat cctctcccag tgattctatt cttttcaatg
     1741 cgttgaaatc cagaaatcct atcaggtctg aattggccag gatggcgaag caggtcttgg
     1801 gattcttctg gcggatgaag accttgtcgc tgcctttcat ccagttcagg aaatcaggaa
     1861 aatcgtcagg ggcgatgacc tcggcggtat gacctctttt ctccaggaag aatttcagag
     1921 ccaggcagga gccgatggca tctccgtcgg gacgggtatg tgccaccagg gcgatgttgc
     1981 ggggtgtgct gaggaactca aatagctccg ggaggttctt ctcgaggcgc atcggggggt
     2041 aaaggtagtg gaaatgcgga atgcggaggg gtaaggagtt ggcggtcggc agctgacagt
     2101 tggcagggtg gaaaatgaag aatactgagg ggaggacgaa aggcggaagg ggttggcagt
     2161 tggcagggca cttcccacag gtctcattat tcatcactat cctctcacgt cataggttct
     2221 gtattccgtg accttggtgc ctccttagtg cctttgtgtt aaaatgtgga acactatggc
     2281 acgggtcatc gctatgctct gacaaacaca aaggacacaa ggggcccttg agaaatgagc
     2341 atggaaactg aggaaagaga ttcccgataa ccaccttcag gaatgatgta ttgggttaag
     2401 ttaatcagtc caaaaccaat aactaataac caacaaccac caactaatcc ccctaaatcc
     2461 cctacctttg cggccaaaat tgaacgacaa tggcaggtaa tatcacattg accatgatca
     2521 agcccgatgc ggtgaagaac aaccacatcg gcggtattct gaagatgatc aacgaagccg
     2581 gattccgcat ccaggctatg aaatacaccc ggatgacccg cgaagtggcc ggtgcattct
     2641 atgcggttca caaagagcgt ccgttctacg gcgagctggt ggacttcatg agcagcggtc
     2701 cgatcgttgc tgctgtgctg gtgaaagaca atgcagtgga agatttccgc aagctgatcg
     2761 gtgccaccaa cccggctgaa gctgccgacg gtaccatccg taaaatgtat gctaccagta
     2821 ttggcgaaaa tgccgtgcac ggttctgaca gtgatgagaa tgccgcaatt gaggcaggct
     2881 ttcacttcag cgcatacgaa aggttctgaa taattagcta atgga
//
LOCUS       DAOMAX010000319         2912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7222_length_2912_cov_3.731537, whole genome shotgun sequence.
ACCESSION   DAOMAX010000319 DAOMAX010000000
VERSION     DAOMAX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7222_length_2912_cov_3.731537"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PK742_12210"
     CDS             <1..842
                     /locus_tag="PK742_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77399.1"
                     /translation="TASGQEFYLFTLHLKATSGDDAQRAREVDSLRKVTDLIPDSIPF
                     IVSGDFNIYDENEPAYNGLLDTSGGGYFIDPLQDSLSTFWNNSGNAAYHTQSTRVRNF
                     DGGSTGGLDDRFDMILFSSAIDAPGGMDYVFGSTVAFGNDGNHYNDSINAMPNTAVTE
                     EVANALHLASDHLPLKVLFTMTWTEEEDTTIVDTTTNSIPDLHSLFRVYPSPANTFIH
                     VASEHYPAELLLMDMQGHMILQTTTDSEDFVLELPGLSDGNYLLLWRYGQHTYMRTLP
                     VLH"
     gene            complement(1241..2392)
                     /locus_tag="PK742_12215"
     CDS             complement(1241..2392)
                     /locus_tag="PK742_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQU77400.1"
                     /translation="MHRVYLDNAATTQLDPAVLDAMLPYLREHFGNPSSIHAYGRETR
                     AAIEKARKKIATILSVSPGEIFFTSGGTEANNTALYCSVRDMGVKRIISSPIEHHAVT
                     HTVESLEKDHGVRADWVQLHADGSVNYEHLETLLADKSAGKTLVSLMHANNEIGILLD
                     IERVGELCRQYDALFHCDTVQTIAHYPINLSKINVHFITAAAHKFHGPKGVGMLYISG
                     DVSIKPLIHGGAQERNMRAGTENLYGIIGLAKAMEMAYDELDTTATYIKGLRKMMADA
                     LLEHFPGVRFNNPEDGLYTVLNVRFPAHPKNDLLLINLDIQGVCCSGGSACSSGSDTG
                     SHVINALRPETGSASVRFSFSKFNTEADVHFTIEQLKSILQPETAASNA"
     gene            complement(2417..>2912)
                     /locus_tag="PK742_12220"
     CDS             complement(2417..>2912)
                     /locus_tag="PK742_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQU77401.1"
                     /translation="GDVAEKHGVKHYQAAVGEVNVVTRMKAVGSVIGGEGNGGIILPE
                     LHYGRDALVGIALFLSHLAKYGKSASMLRASYPNYFMAKKKMDLPEGVSVQSVLDQVA
                     EKYTQYPQNKEDGLKITMDKEWVHLRASNTEPIVRIYAESDSPVTAENIAARLMQDMS
                     EITK"
BASE COUNT          667 a          766 c          732 g          747 t
ORIGIN      
        1 ataccgcgag cggacaggag ttctaccttt tcaccctgca cctgaaggcg accagcggtg
       61 atgatgccca gcgggcaaga gaggtggaca gtctgcggaa ggtaacagac ctgatcccgg
      121 acagtatccc gttcattgtt tcgggcgatt tcaatatcta cgatgagaat gaaccggctt
      181 ataacggctt gctggatact tcagggggtg gctatttcat agatccattg caggacagcc
      241 ttagcacctt ctggaacaac agtggcaatg cagcttacca tacgcagtct acccgcgtgc
      301 ggaatttcga tgggggcagt accggcggac tggacgaccg gttcgatatg atcctgtttt
      361 catcagccat tgatgcaccc ggaggcatgg actatgtgtt cggttctacg gtagcctttg
      421 gcaacgacgg caatcactac aacgattcca tcaatgccat gccgaatact gcggtcactg
      481 aagaagtcgc caatgcattg cacctggcat ccgatcacct gccgctgaag gtgttgttca
      541 ccatgacctg gacggaagag gaagacacga ccattgttga taccactacc aactcaattc
      601 ctgatctgca ttccctgttc cgggtgtatc caagtcccgc aaataccttt attcatgtgg
      661 cttccgaaca ttacccggct gagctgctac tcatggatat gcagggtcat atgatcctgc
      721 aaaccaccac tgattccgaa gatttcgtcc tggaattgcc gggattatct gacggtaatt
      781 acctgttgct ctggcgatac ggacagcata cttacatgcg taccctgcct gtcctgcatt
      841 aggacaatca taatctgatt gtccggattt atcagaagcc cttgcaggta gcaaccgaac
      901 caacaaaggt cagttagcag aggaaccgtt cccccggcgg cagtgcgtgt gttgaagcat
      961 cccaagcgct gggttctgca ttgcgttccg agtcgctatt ctatggatca ttttattgcg
     1021 gtgtttcaga aggatcagcc ttgaccgaca gtcattgcga agaaggaatg ctcagcgccg
     1081 caggatgcgt tgccgggccg caagagcacc ttcggcgggg gaaggcgttt tggtgatacc
     1141 ttttgcgctt caaaaggtat cgaaacaata ttgacagacg aatctattga tcaacgctca
     1201 actattcttg tagcatatcc ttcaatttgc aggcttccca ttaagcattt gatgcagctg
     1261 tttccggctg caggatactc ttgagctgtt cgatcgtgaa atgcacatcg gcttcggtat
     1321 tgaacttgct gaaagagaac cgaacagatg cggagcccgt ttcaggccgg agcgcattga
     1381 tcacatgaga acccgtgtca ctgccgctgg agcaggccga tccgccactg cagcacactc
     1441 cctgaatatc gaggttgatg agcagcaggt cattcttcgg gtgcgcaggg aaacgcacat
     1501 tcagtacggt gtataaacca tcttcaggat tgttgaagcg cacaccgggg aaatgctcca
     1561 gcagcgcatc agccatcatc ttccgaagac ctttgatata agttgcagta gtatccagtt
     1621 cgtcataggc catctccatg gctttggcca ggccgatgat accgtacagg ttctctgtac
     1681 cggcccgcat attgcgttcc tgggcaccac catgaataag cggtttgatg gacacatctc
     1741 cgctgatgta cagcatgccc acaccctttg gtccgtggaa tttatgcgca gccgccgtga
     1801 tgaagtgaac attgatctta ctcaggttga tggggtagtg ggcgatggtc tgcacggtat
     1861 cgcagtgaaa gagtgcatcg tattgcctgc atagttcacc cacccgttca atgtccagca
     1921 gaatgccgat ctcgttgttg gcatgcatga gactgacaag ggtcttgcct gcacttttgt
     1981 cggcgagcaa tgtctccaga tgttcgtagt tcactgaacc atcggcatgc agttgcaccc
     2041 agtctgcacg aacgccatga tccttctcca gcgattccac cgtatgcgtc accgcatgat
     2101 gttcaatggg agaggagatg atgcgtttca cacccatatc ccgcacactg cagtacagtg
     2161 ctgtattatt tgcttctgta ccaccggaag tgaagaagat ctctcccgga gaaacagaca
     2221 atatagtagc gatcttttta cgtgctttct ctatggcagc tctggtctct ctgccgtagg
     2281 catggataga actgggattg ccgaaatgct cccgcaaata aggcagcatg gcatcgagca
     2341 ccgcaggatc cagttgggtc gtcgcggcgt tatctaaata tactctgtgc atgggtgtgt
     2401 caatacaagt tagcgcttat ttcgttatct cgctcatgtc ctgcatgaga cgtgcggcaa
     2461 tattctctgc ggtcaccggc gaatcactct ctgcatagat acgtacaatg ggttctgtat
     2521 tgctggcacg gaggtgcacc cattccttgt ccatggtgat cttcagtccg tcttctttgt
     2581 tctgcggata ctgcgtgtat ttttctgcta cctggtccag tacactttgt acagatacac
     2641 cttccggcag gtccatcttt ttcttggcca tgaaataatt cgggtaggaa gcccgcagca
     2701 tggaggccga tttgccgtat ttcgccagat gtgaaaggaa cagagctatt cccaccagcg
     2761 catcgcggcc atagtgcagt tccggcaaaa tgataccgcc gttcccttcg ccaccaatga
     2821 ccgagccgac cgctttcatt ctggtgacca cattcacttc tcccacagct gcctgataat
     2881 gttttactcc gtgtttttcg gcaacatctc cc
//
LOCUS       DAOMAX010000320         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7309_length_2896_cov_4.245336, whole genome shotgun sequence.
ACCESSION   DAOMAX010000320 DAOMAX010000000
VERSION     DAOMAX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7309_length_2896_cov_4.245336"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PK742_12225"
     CDS             <1..543
                     /locus_tag="PK742_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77402.1"
                     /translation="VYGITTPLQTWNPLRAQLDVWKDMAIAIGRAKGIRHQLGILFQA
                     PGWMPEELGGPILPKAVDPLTNKKFDVRIPPSWHAYVLVSFLLILGGASFFLFSLEEL
                     NIRQQLLAAAMVLWSITAMGLILEKQRLAFLAEGIRLLATPVVIWWCSGQMLAAGITT
                     GIMLLSLLCWPVLFRDSRSN"
     gene            1039..1698
                     /locus_tag="PK742_12230"
     CDS             1039..1698
                     /locus_tag="PK742_12230"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQU77403.1"
                     /translation="MKTKASWLINVYLMTAAVHLVLLAAGKENGIFISKVLLMPELAL
                     LVWLNRGLVRTPLLLIALFWCWMGDILLQFSGGQDNFFLFGLASFLLGHLFYMFTFRK
                     LQQGGKLVWWWLIPVAAYVFFLLYTVWPGLGEMRIPVLAYAVVITLMLVFALHRYRGT
                     TAISFSTVAAGAILFIISDSLIAWNTFHETIRYASIAIMSTYALAQYLIVQGLVWSRR
                     D"
     gene            complement(1897..2442)
                     /locus_tag="PK742_12235"
     CDS             complement(1897..2442)
                     /locus_tag="PK742_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77404.1"
                     /translation="MKRTITHAGLILMLLFTAATNVLVAQELENLMAMNTDKPAANNH
                     SAILHTDGNDGLTRSDEVIVSSYFTLAGQFIRPYMVVTNDGVSFDPVLLNGQQQASFR
                     FENDHVEYVTGGKTYRLDISSINQLQNRNGNCLVTGKIEIKVDATKIDGEKVNEVGVF
                     IDHENHISFIKIGKAEYYLEP"
BASE COUNT          761 a          581 c          780 g          774 t
ORIGIN      
        1 gtctatggca tcaccactcc attgcagacc tggaatccgt tgcgggcgca actcgatgtc
       61 tggaaagata tggccatcgc catcggcagg gccaaaggca tcagacacca actcggtatc
      121 ctgttccagg cacccggttg gatgccggag gaactgggag gtcctatcct ccccaaagcg
      181 gtagatccgc tcaccaataa aaaattcgat gtaaggatcc ctccatcctg gcatgcctat
      241 gtgctggtct ccttcctgct gatcctgggc ggtgccagct tctttttgtt ttcgctggag
      301 gaattgaata tccggcagca gttgctggca gctgccatgg tattatggtc catcacagct
      361 atgggattga tactggagaa acagcgcctc gcattcctgg cagaaggcat acgtctgttg
      421 gccacgcctg ttgtgatctg gtggtgcagc ggacagatgt tagctgcagg aataaccacc
      481 ggcatcatgt tgttgtcgct actatgctgg cccgtgttat ttagggattc caggtctaat
      541 taaataagaa caaaaaatcg acgaactata gcatttgata tgaaatggtg gccaaccgtt
      601 ttttgtttgg aaggtttcag gtatgtaacg caggtggaaa aagaaacaaa ttggctgaga
      661 tcgcaaagga acggcttccc ccgacggcag tgcgagggca ggagcatcca gagggcatgg
      721 tcttgcttag cgttctgaat cgccagtctg acgggtaatc gaagcgttgt catttctgaa
      781 gcacgatttt acgggcagct gttgcgatga ggaatgccag gcgccacagg atgcgttgcg
      841 gggtcgcatg agcaccttcg tcgggggaaa gcgttttggt gatacctttt gcgcttcaaa
      901 aggtatcgaa gttaattatt ggagaaagca ttgaatttta atttggttat tacttgatga
      961 ataatatatt ttgactgcca ggttgatctg aatttcagat caggatacct ctaaagcact
     1021 tcttaaacga taaaccacat gaaaacaaaa gcatcctggc tcatcaatgt ctatctgatg
     1081 acggcggcgg ttcacctggt actgctggcg gccgggaaag aaaatggcat tttcatcagc
     1141 aaggtactgc tcatgccgga gctggcattg ctggtatggc tgaacagagg tctggtcagg
     1201 actcccttgt tactgatcgc cctgttctgg tgctggatgg gagatatact gttgcagttc
     1261 agtggcggac aggacaattt ctttctgttc ggactggcgt ccttcctgct cgggcatctg
     1321 ttctatatgt tcactttcag aaagctgcag caaggcggaa agctggtgtg gtggtggctg
     1381 attccggttg ctgcctatgt gttctttttg ctctatacgg tctggcccgg gctgggagag
     1441 atgcgcatac ctgtgctggc atacgcagtg gtcatcactt tgatgctggt gtttgcactg
     1501 cacagatata ggggaacgac agcgatctca ttcagtacag ttgctgcagg agccatactg
     1561 ttcattatca gtgattcgct gatcgcatgg aatacctttc atgagaccat cagatatgct
     1621 tccattgcca tcatgagtac ctatgcacta gcgcaatatc tgatcgtaca gggactggtt
     1681 tggagcagga gagattgagt gggagggaga gagggagtgg gagtgagagt gagtgagagt
     1741 gggagtggga gtgggagtgg gagcgattga gtgtgagtgt gagagggagt gggagtaatt
     1801 gagagtgaga gtgagtggct gtcggggtct tccgctacat aacataaaaa agtcccggag
     1861 gagcgggact ttttcgtcat gatcagataa atcgtatcag ggctcgagat agtactctgc
     1921 cttgccgatc ttaataaaag agatatggtt ctcgtggtcg atgaaaacgc ccacttcatt
     1981 cactttttca ccatcaattt tggtagcatc caccttgatc tcgatcttgc ctgtcacgag
     2041 gcagttacca ttccggttct gtaactggtt gatggatgag atgtcgaggc gataggtctt
     2101 gccaccggtc acgtattcta cgtggtcatt ttcaaacctg aaactggctt gctgctgacc
     2161 attaagtaat acagggtcaa aggaaacacc gtcgttggtg accaccatgt atggacgaat
     2221 aaattgtccg gccagggtga aataactgga aacgatgact tcgtcagatc tggtaagacc
     2281 gtcatttcca tccgtgtgca ggatagcaga gtggttatta gcagcaggct tatctgtatt
     2341 catggccatc aggttttcca gttcctgagc cacaagcaca ttggttgctg cagtaaaaag
     2401 cagcatcaaa atgagacccg catgtgtgat agtccttttc atggctgaaa agttgtacac
     2461 aagattaacc agctattcag agatatttat tacactatta aataaaaagt gacaataata
     2521 aatgaaaaaa tttgtctctg gtgatcaatt aagcagtaat aataggaaaa attaagcaaa
     2581 aaagtgaata gcttatgatg tttttgcaga cgaagacgaa tgtcatttgg ctgatatgtg
     2641 acattaaaaa aatagcttta ataacagcta atatggctaa aatggtggct agtagtacaa
     2701 agttagttca aaaaaatagg atatagtgac tctcgtatga agaataagac accttcccat
     2761 atactgtgaa aacgtagtca tggcagggca atagccggtt ttaatcagtt agcaaataag
     2821 cagtcgccta tggcagaagc atatgagtga agcagtgagc ggtcaggtgt gaatggtgag
     2881 gcactccgta tgatat
//
LOCUS       DAOMAX010000321         2893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7319_length_2893_cov_4.552149, whole genome shotgun sequence.
ACCESSION   DAOMAX010000321 DAOMAX010000000
VERSION     DAOMAX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7319_length_2893_cov_4.552149"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..>2893)
                     /locus_tag="PK742_12240"
     CDS             complement(181..>2893)
                     /locus_tag="PK742_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQU77405.1"
                     /translation="GVHWYPRICVYDRKFGWTTDQHLGKEFYGDFGHFDVRLDMPDQY
                     IVGATGTLINPEEAMPEGLRKKLDIKNFADKPWGSEPSIIIPVTGGRKVWHFEAVNVH
                     DFAWTADPTYRIGEAVAIVDGRFVLCQALAQEQHASGWQNAADYTTQIIETFSRDFGS
                     YIWPKIIVADARDGMEYPMLTLDGGFDPYYRDLLIHEVGHMWFFGMLGNNETYRAMMD
                     EGFTQFITAWGYKAIDGNVKIGYPETKKWKAKFREAEDVMLTENYYGYLRDAVRQNDP
                     PLNTHSDHFGSALGHGGGYGHVYYKTAVMLNNLQYVLGDELFLEAMQHYVQQWKTAHP
                     YPEDFRNSIIQYTGADLNWFFDQWMETNKNIDYAITDVKPGKVSAAAMEEGITLRPYS
                     VTFERKGSMQMPLDFIAISKTGDTMRYYIPNTWFEKKVNPDPPVNERLNRLALENVNV
                     LPVWIGWDKLNPTYTAEIMVNGDLDRIEIDPSRRMADIDMLNNSSKCPVDIGFDSRVW
                     NWPDPYHYTMKWRPDLWWNEVDGFKAGVHLNGNYFNYKHIFSLTAWVNSGLGQGSPYY
                     LGDDGTEGPSYEQDPFSINFSYKTATDKLVHASDLEMNFRYLEGILGGQAGFVHRLGD
                     REQNDLRIYFKAFKETNSDYHADNFSGLWNNSMNIEYTHRYSYPKGNGTIVAGMRSTA
                     WLSETDFNRVNLEAVNNNKLGKFDLRTRFFAQGATSTGGAPLESRLYLAGANPEEMLD
                     NKYTRTFGWFDRDWMYPGIETSHFQYGGGLNLRGYAGYLGAQTGTDGNVYNIYNGLNG
                     ASVNAELAFNRLFGKKLIQAIDFVPYLFADAGTLSYQEEDGKQYFADLRADAGIGATV
                     SWKYWGPLEQVKPLTIRIDLPLWLNRPPYAEEDYLKFRWVLGVERAF"
BASE COUNT          682 a          769 c          676 g          766 t
ORIGIN      
        1 cagtcagcag tcagcagtca gcagtcagca gtcagcagtc agcagtcagc agtcaatttg
       61 gtcatacaag ttagaactat tccgtcaata gctatcggga cgtgtcaact ggcacatcag
      121 caaatcggca catcggccaa tcggctaatc aactaattgg ctcatcggct aatcaactaa
      181 tcaaaaggcc ctttccacgc caagcaccca tctgaatttc agatagtctt cttccgcata
      241 tggaggcctg ttcagccaca gcggcagatc tatccggatg gtcagtggct tgacctgttc
      301 cagcggaccc cagtatttcc aggatacagt tgcaccgata cctgcatcag cccgaaggtc
      361 agcaaaatat tgcttgccat cttcttcctg atagctcagg gtacctgcat cagcaaacag
      421 atagggtaca aagtctatgg cctgaatcag tttcttgcca aacaaccggt tgaaggcaag
      481 ttctgcattc acagaagcgc cattcaatcc gttgtaaata ttatagacat tgccgtcagt
      541 accggtctgg gcacccaggt agcctgcgta tccgcgaagg tttagtccgc cgccatactg
      601 gaaatgactg gtctcaatac caggatacat ccaatcgcgg tcaaaccatc caaaggtcct
      661 tgtgtatttg ttgtccagca tttcttccgg attggcaccg gccagataca atctggattc
      721 cagcggtgct ccgcctgtgc tggttgcacc ttgcgcaaag aagcgtgtac gcagatcgaa
      781 cttgcccagt ttgttgttgt tgaccgcctc caggttgacc ctgttgaaat ctgtttcact
      841 caaccaggca gttgagcgca tgcccgcaac aatggtaccg ttccctttcg gataactgta
      901 acggtgggta tattcaatgt tcatggagtt gttccagagt ccgctgaagt tatcggcatg
      961 atagtctgaa ttggtctcct tgaatgcctt gaaataaatg cgcaggtcgt tctgttctct
     1021 gtcgcccagg cgatgtacga atcccgcctg tcctcccagg atgccttcca ggtaccggaa
     1081 attcatttcc aggtcagatg catgcaccag tttgtctgtg gctgtcttgt aggagaaatt
     1141 aatgctgaac ggatcctgtt cgtaagaagg tccttccgtt ccgtcatccc ccaggtaata
     1201 cggacttccc tgtccgaggc cggagttgac ccaggcggtc aggctgaaga tatgtttgta
     1261 gttgaaatag tttccgttca ggtgcacacc tgccttgaat ccatccactt cattccacca
     1321 caggtccggt cgccatttca tggtatagtg atacggatca ggccagttcc agacccttga
     1381 atcaaaccca atatctaccg ggcacttgct gctgttgttc agcatatcaa tatcggccat
     1441 ccggcgacta ggatcaatct caatgcggtc cagatcgccg ttcaccatga tctcagctgt
     1501 gtaggttgga ttgagtttgt cccagcctat ccagacaggt aatacattca cattttccag
     1561 tgccagtctg ttcagacgtt cattaaccgg cggatccgga ttgaccttct tctcaaacca
     1621 ggtgtttgga atataataac gcatcgtatc tccggttttg gagatcgcaa tgaaatccag
     1681 cggcatctgc atactgcctt tcctttcgaa ggtcactgag taaggacgca gggtaatgcc
     1741 ttcttccatc gctgcagcac tcacttttcc cggtttcaca tctgtgatgg cgtagtctat
     1801 gttcttgttg gtctccatcc actgatcgaa gaaccagttc aggtcggcac cggtgtactg
     1861 gatgatgctg ttcctgaaat cttccgggta ggggtgagcg gtcttccact gctgcacata
     1921 atgttgcata gcttccagga atagctcatc gcccaacaca tactggagat tgttcagcat
     1981 gaccgcagtc ttgtaataca cgtgaccata gccaccgcca tgtccaagcg cagaaccgaa
     2041 atgatcgctg tgggtattga gcggtggatc gttctgtctg actgcatctc tgagataacc
     2101 gtaataattt tcggtcagca tgacatcttc cgcttcacgg aatttcgcct tccacttctt
     2161 ggtttccgga taaccgatct tgacatttcc gtcaatcgct ttgtatcccc atgcggtaat
     2221 gaactgggta aatccttcgt ccatcatggc acgataggtc tcgttgtttc ccagcattcc
     2281 gaagaaccac atgtgaccaa cttcatgaat gagcaggtcg cgatagtagg ggtcaaatcc
     2341 accgtccaga gtcagcatcg gatattccat gccatcacgg gcatctgcca caatgatctt
     2401 gggccagata taggagccga aatcgcggct gaaggtttcg atgatctgtg tagtataatc
     2461 ggccgcattc tgccagccgg aagcgtgttg ttcctgggcc agcgcctggc ataaaacaaa
     2521 gcgcccatcc acaatagcca ctgcttctcc gatccggtag gtagggtctg cagtccaggc
     2581 aaaatcatgt acgtttacgg cttcaaaatg ccataccttc cggccaccgg taaccggaat
     2641 gatgatactg ggttctgaac cccatggttt atctgcaaaa tttttaatgt ccagtttttt
     2701 ccgaagtcct tccggcatgg cctcttccgg attgatgagg gtgccggttg cacctacgat
     2761 atactgatcg ggcatatcaa gccgcacgtc gaaatggcca aaatcaccat agaattcttt
     2821 tcccagatgc tgatcggtag tccatccgaa cttgcggtca tatacacaaa tgcgtggata
     2881 ccagtgcacg cca
//
LOCUS       DAOMAX010000322         2883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7359_length_2883_cov_4.496110, whole genome shotgun sequence.
ACCESSION   DAOMAX010000322 DAOMAX010000000
VERSION     DAOMAX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7359_length_2883_cov_4.496110"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..2445
                     /locus_tag="PK742_12245"
     CDS             286..2445
                     /locus_tag="PK742_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77406.1"
                     /translation="MIDPSQVQSGFDSETLFNRRYIRYLLMSSIETGSLPLQITVPIP
                     GPGGTPINVTIDLYVPTDYDRNYTPDAQASPPVNTSSQAFATEILFNDPTGGDLKVTM
                     VANVTGFAPGQVVDIYMTFQLLVDTDENGNQSNARLSLTTVDVQGPMIQFILSTGQVT
                     QQDIINIFRQFVDRDVPLSMVGSSVQKVEMQKLQGADIDTHVIAIYLNLILKNGPADD
                     DIRGDRGDISNGINFLPDGDDIAFGMPSNIYNRLSTDAFERMAEETSEGSGSFTHPIP
                     NPDDPDGDPLGRIRSITIRPKQSGGTFSDELEIVVDGEIIWDWTIFGVDVTPDPDFKL
                     YITITPVITNGIISWDLSYDLDFDPVLKFLGNLIIAGLMTLLFVPTLGWGSLIVGGIG
                     FALLYGMEEWIVEPLIANGIGDSAGMDTSFLDSIPNRLTIESRRWDPFYFTDHQIVAA
                     TDGMAFRDTGLGFSGQAKLDRVARFVTHIVARTEERNGDGEIIGLRYRVKDHTNSAND
                     FVNHFAGTDRESFTQVTNDAETNLYALDLAEITGRIANGKLIPRILYLAQNVHIKDNQ
                     VEEIAAITNREVDEIINRLIDDFEEVKETEIRASDEDVLRDEALEELEEELDDTPTND
                     QVEERLQEKIDELVAEALDEYALDELDTDLEDAVREQTRFDMAPFEMGDLQLKKILLL
                     YRFDLVIRKGKPYYRDRPDGYIPDNLMELPGFTPDDD"
     gene            2520..>2883
                     /locus_tag="PK742_12250"
     CDS             2520..>2883
                     /locus_tag="PK742_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HQU77407.1"
                     /translation="MSDLFLRMEELPSLQLAAKGITMTMAENRTVKLWQSAKPIMMRM
                     QGRRPGFISMEVFPEGINFENFTPRTPFQKWAAVQLDEGAAVPEGLEAYSLQGGPYAV
                     FRFRGLPSQVAALMQHIFL"
BASE COUNT          738 a          708 c          739 g          698 t
ORIGIN      
        1 caaatggtca ggtactatgt ttgcatggtc tatcgtaaga cgtgaactaa ctgagtactt
       61 tggagaccga ttttctaacg ctgacacagt taattgaaca ctcccagcta tcggggctca
      121 ctctcactct cactctcact ctcactctca cacctcactc ctctctgctc attcctcacc
      181 ctgtcccaaa gactctttta ttcatttata attaattcca cagctatgct atcccaccgc
      241 atcattatgt agatttacct attccatcac cctcaatcat atgctatgat cgatcccagt
      301 caggtacaat ccggttttga cagcgagacc ctgttcaaca gacgttatat ccgctatctg
      361 ttaatgagca gcatagagac cggttcactg cccttacaga taacagtacc cattcctggt
      421 ccgggaggca cccccatcaa tgtgaccata gacctgtatg tgccaacaga ctatgaccgc
      481 aactacacac cggatgcgca ggcatcaccc cccgtcaata catcatcaca ggctttcgct
      541 acagagatcc tgttcaatga tccaacgggc ggcgacctga aggttaccat ggtagcaaat
      601 gtaacgggct tcgcaccagg tcaggtagtg gatatttaca tgacctttca gttgctggta
      661 gatactgatg agaacggcaa ccagtcaaat gcacgtttga gtttgaccac ggtagatgtg
      721 cagggtccga tgatccagtt catcctttct acaggccagg ttacgcagca ggatattatt
      781 aatatattca gacagtttgt tgaccgcgat gtaccattga gtatggtggg aagttcggtg
      841 cagaaggtgg agatgcaaaa actgcaggga gctgatatcg atacccatgt cattgccatt
      901 tatctgaacc tcattctgaa gaatggcccg gccgatgatg atataagagg cgacaggggt
      961 gatatatcca atggcatcaa ctttctgccc gatggtgatg acatcgcctt cggaatgcca
     1021 tcaaacattt ataaccggtt aagcacggat gcttttgaac gcatggcaga agagaccagc
     1081 gagggaagtg gcagcttcac ccacccgata ccgaacccgg atgatccgga tggtgacccg
     1141 ctcggccgga tcagaagcat caccattcgt cccaaacaaa gtggtggcac cttcagcgat
     1201 gaactggaga tcgtagtgga cggagagatc atctgggact ggaccatttt cggagtggat
     1261 gtaacccctg atccggattt caaactctat attaccatca cgccggtcat caccaacggt
     1321 atcatcagct gggacctctc gtatgacctc gactttgacc cggtgctgaa attcctggga
     1381 aacctgatca ttgccggact aatgaccctg ctgttcgtac ctaccctcgg gtggggttct
     1441 ctgatcgtag gtggtattgg attcgcattg ctttacggaa tggaagaatg gatcgtggag
     1501 ccgctgatcg ccaacggtat aggcgacagt gcaggtatgg ataccagttt tctggacagc
     1561 attcccaacc ggctgaccat cgagtcgcga cgctgggatc cgttttattt caccgaccac
     1621 cagatcgtgg cagccaccga tggtatggca tttcgtgata ccggactggg attttccggt
     1681 caggctaaac tggacagagt ggcacgtttt gttacgcata tcgtggcacg tactgaagaa
     1741 cggaatggcg atggcgagat catcgggctg cgctatcgcg tgaaagacca taccaacagc
     1801 gccaatgatt ttgtgaatca ctttgcagga actgacaggg aatcgttcac gcaggttacc
     1861 aacgatgcag agaccaacct gtatgcactt gatctggctg agatcaccgg caggatagca
     1921 aacgggaaac tcattccccg catcctgtat ctggcacaga atgttcacat aaaggacaac
     1981 caggtagaag agatcgccgc catcaccaac agggaagtgg atgagatcat caaccggttg
     2041 atagacgatt ttgaagaggt caaagaaaca gagatccggg cgtcagatga agatgtgctg
     2101 cgtgatgaag ccctggaaga actggaggaa gagctggacg atacacccac caatgatcag
     2161 gtggaagaaa ggctgcagga gaagatcgat gaactggttg cggaagcact ggatgagtat
     2221 gcacttgatg aactggatac cgatctggaa gatgccgtaa gagaacagac ccgctttgat
     2281 atggcaccat tcgaaatggg agatctgcag ctgaagaaga tcctgctgct gtaccgattt
     2341 gacctggtga tcagaaaagg caaaccttat taccgcgacc gtcccgatgg ctatataccc
     2401 gataacctca tggagctgcc aggatttacg ccggatgatg attgaggtgg agggggagtc
     2461 gttgttcgtt attcgttgtt cgttgttggg ccatctccgt taacttagcc tgttatatga
     2521 tgtctgatct ttttctgcgg atggaagaat tgccatcact tcaacttgct gccaaaggca
     2581 tcaccatgac gatggctgag aaccggacag taaaattgtg gcagtcggcc aagccgatca
     2641 tgatgcgcat gcagggcaga cgtccgggtt tcatttcgat ggaagtgttc cctgagggaa
     2701 taaactttga gaacttcacc ccccgaacac cctttcagaa atgggctgct gtgcaactgg
     2761 atgaaggcgc tgcggtgcct gaggggctgg aagcctattc gctgcagggt ggaccgtatg
     2821 cagtgttccg tttccgcgga ctgccgtctc aggttgctgc tctgatgcag catatttttc
     2881 tgg
//
LOCUS       DAOMAX010000323         2877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7391_length_2877_cov_6.744862, whole genome shotgun sequence.
ACCESSION   DAOMAX010000323 DAOMAX010000000
VERSION     DAOMAX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7391_length_2877_cov_6.744862"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK742_12255"
     CDS             complement(<1..385)
                     /locus_tag="PK742_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77408.1"
                     /translation="MSMNLHGQNELQFSFECGESIKSPIESSVLLKFPDGRISKLYLD
                     SKPKYDFIDKEYFTSKGKYILSIYFIAENYGQDSIEYDFELNGDEIKTTISVNFDFKE
                     RLIKKGNIYEKGDEVLNGYVRINKYY"
     gene            complement(675..1961)
                     /locus_tag="PK742_12260"
     CDS             complement(675..1961)
                     /locus_tag="PK742_12260"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQU77409.1"
                     /translation="MCRKTPLRSASDQWDGYPVSDTNSIGALFRAHGERYIQAYKPHQ
                     RAIAFIRSVRLCRTPGLGRIVYTCKGCGEQVTIYKSCGNSQCPVCQSIKREQWADALQ
                     GKLLRIPYVHLVFTMPHQLNGLARSNKSAVYSILMRAAWKTVQAQMKDKTVLPGMTAV
                     LHTFGSDMKYHVHVHALVTFGGLEPSGKWVYPTHRYWIASFRKMCGTFKAISLQLIRK
                     DIAQKKITSQQGNEDLLHEVGKIRWVVHSTRATTQTDVLKSYLARYINRIAISNNRLK
                     YLAQTDEVAILYNDYRNQQNECAAPKAIKHLDPLTAIHQIIQHVLPRHFQKSRHYGLH
                     HTCTLIRNQAEEAIKAHPATVRTALQIITALLKRELYQCDYCQGQSFIKTEYPPDRIY
                     LGQYLAPGTERAPPTSWRRPVHQRADRSTTAYPSFA"
     gene            complement(1886..2770)
                     /locus_tag="PK742_12265"
     CDS             complement(1886..2770)
                     /locus_tag="PK742_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQU77410.1"
                     /translation="MRRQLKYIYDHHLDDFEEFMVLKNFSRRTVTAYMQAVYQFLDWW
                     DDNCPDIPMSDDVVRKYLVFRFESNKAWQTVNSDYSAIQKWFKNVLMLRWDIQKLPRP
                     RKERQLPRVFSREDIVKIIEGASTFKHQVLLTFIYVTGVRLSEAIHMRIDDVDGNRKQ
                     IRIAKGKGGKDRYVLIPDVLLELLRTYYLRERPEEYLFNGKNKGKPLSQRAVQWALQE
                     AKKNAGVKRPGGIHTLRNCYATHHLEGGTDLVFLQEQMGHKQLKTTIRYIGLCVERHR
                     YVQHPINGMAIRYQIPTA"
BASE COUNT          694 a          674 c          667 g          842 t
ORIGIN      
        1 tatagtattt attaatccga acatacccat ttagaacttc atctcctttt tcatagatgt
       61 ttcctttttt aataagtcgt tccttaaagt caaagttcac tgaaattgta gtttttattt
      121 catctccatt taactcaaaa tcatattcaa tcgaatcttg tccgtaattt tcagctatga
      181 aataaattga aagaatatat tttccttttg aagtaaaata ttctttatca ataaaatcat
      241 acttaggttt agaatctaag tacaattttg aaatacgacc atcaggaaat tttaataaaa
      301 cgctcgattc aatcggtgat ttaattgatt ctccgcattc gaatgaaaac tgcaattcat
      361 tttgtccatg cagattcatg ctcattaagc aaacgaagaa aattattatc aatctgtttg
      421 tcatcattgg tacgtcgttt tttcactgac cgctaactcc aaggaaagtt gtcgcccctt
      481 gcgacaactt ttcgagttga acgagaccct ttggtgtata gggaaacgct ctccaatcgg
      541 aactctgatg atcgacaaag gcttatacca aagataaggt atttgctcga ttgatgcatg
      601 tcctgagctt tgccgtatgg tttggcctga agggttgatc tatttccttg tcaatgtggc
      661 tgttttacgt gccatcaggc aaaagaagga taggcggtag tcgaacgatc cgctctttga
      721 tgaaccggtc tgcgccagga tgtcggtggt gctcgttctg ttccaggggc caggtattga
      781 cccagataga tccggtccgg tggatattct gtcttgatga acgactgacc ctggcagtag
      841 tcgcattgat acagttctcg tttcagcagt gccgtgatga tctgcagtgc agtgcgtact
      901 gtggccggat gtgccttgat cgcttcttct gcctgattcc gtatcagcgt gcaggtgtgg
      961 tgcagaccgt aatgccggct cttctgaaaa tgacggggca agacgtgctg aatgatctga
     1021 tggatggccg tgagcggatc caggtgcttg atggccttgg gtgccgcaca ttcattttgt
     1081 tgattgcggt aatcgttgta caggatggcc acttcgtccg tttgagccag atatttcagg
     1141 cggttattgc tgatggcgat gcggttgata tagcgggcca gatacgattt gagcacgtcc
     1201 gtttgagttg tggcccgggt ggaatggacc acccatctga tcttgccgac ttcatgaagc
     1261 agatcttcat tcccttgctg gctggtgatc ttcttttggg cgatgtcctt tcggatcaac
     1321 tgaagactga tggctttgaa cgtgccgcac atcttgcgaa acgaagcgat ccagtaacga
     1381 tgtgtcggat agacccattt gccagacggt tcaagcccgc caaacgtgac cagtgcgtga
     1441 acgtggacat gatacttcat atccgatcca aaagtgtgga gtacagccgt catgcccggg
     1501 aggaccgttt tatctttcat ttgtgcctgg accgtcttcc aggctgctcg catcaggata
     1561 ctatagactg ctgatttatt gcttcgtgcc aggccattga gttgatgagg catcgtaaac
     1621 accaggtgga catacgggat gcgcaagagt tttccctgca aagcatccgc ccactgctca
     1681 cgtttgatcg actgacaaac cggacattga ctgttgccac agctcttgta gatggttacc
     1741 tgctccccgc agcccttgca ggtgtaaacg attcgcccta gtcccggagt ccgacacaaa
     1801 cgcactgacc gaatgaaggc aatggcacgt tgatggggct tgtaggcttg gatatagcgt
     1861 tcaccgtgtg ctctgaacaa cgcacctatg ctgttggtat ctgataccgg atagccatcc
     1921 cattgatcgg atgctgaacg tagcggtgtc tttctacaca tagaccgata taccggatgg
     1981 tggtcttcaa ttgcttatga cccatctgct cttgtagaaa aaccaaatct gtgccacctt
     2041 caaggtgatg tgtggcgtag caatttcgca aggtgtggat tccacccggt ctcttgacac
     2101 cggcattctt tttggcttcc tgcaacgccc actggactgc tcgctgtgac aatggcttcc
     2161 ccttgttctt gccattaaac aggtactcct ccggtcgctc gcgcaaataa tatgtccgca
     2221 gtagttccag caagacatcc ggaatcagga catagcggtc cttgcctccc tttcctttgg
     2281 cgatgcggat ctgcttgcga tttccgtcca catcgtcaat gcgcatgtgg atggcctcac
     2341 tcaaccggac accagtgaca taaataaagg tcagcaatac ctgatgttta aatgtcgaag
     2401 cgccttcaat aatcttgaca atatcctccc tggaaaatac ccgtggtaat tgtctctctt
     2461 ttctgggacg aggtaatttc tgtatatccc accgaagcat caatacattt ttgaaccact
     2521 tctgaatggc cgaataatca ctattgaccg tttgccaggc tttgttactt tcgaaccgga
     2581 ataccaggta cttgcgcacc acatcatcac tcattggaat atccggacaa ttgtcgtccc
     2641 accagtccag aaattggtac acggcctgca tatatgctgt caccgtcctt ctggaaaagt
     2701 ttttcaaaac catgaactct tcgaaatcat ccaggtgatg atcgtagatg tatttgagct
     2761 gcctgcgcat aagatggatc gggttggtgc aggccaaatc tatatgtaca acaggcaatt
     2821 tgtcaatagc tcaatctgcg cataatcgga tttattgagg gggcttgttc aacggtt
//
LOCUS       DAOMAX010000324         2853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7497_length_2853_cov_4.361330, whole genome shotgun sequence.
ACCESSION   DAOMAX010000324 DAOMAX010000000
VERSION     DAOMAX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7497_length_2853_cov_4.361330"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PK742_12270"
     CDS             <1..1014
                     /locus_tag="PK742_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQU77411.1"
                     /translation="DKYFENCREKGYDDALTKDVWHQIESFAGYSFAKGHSASYAVES
                     YQSLFLKTYYPLEFMVGVINNFGGFYSTELYVHEARMAGGRIEAPCVNNSLYATTIGG
                     KTIHLGFQHIKSLEHKIATGIPEERLQHGPFLSLDDFLHRVSITLEQLEILILINAFR
                     FTGISKQELLIEAYMRMHKTRRTVPSLQLFAAQPDQPYQFPSLHSDMLEDAWDQVKLL
                     GFPLASPFTLIDPEVLEDPDLVCARQLPYLNGQEVEVVAYYVTRKVTYTIKGEVMFFG
                     TFIDRNGEWVDTVHFPDVAKQYRFRGKACYRIRGKVTEEFGTWSIEAHYLEMIPMLLP
                     KGI"
     gene            1060..>2853
                     /locus_tag="PK742_12275"
     CDS             1060..>2853
                     /locus_tag="PK742_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQU77412.1"
                     /translation="MQNFSKRSPLSEKVVRKKQAPLPESSPDIPYLEVRGAREHNLRN
                     IDVSIPRNSLVVITGVSGSGKSSLAFDTIYAEGQRRYMETFSSYAQQFVGNMERPEVE
                     EITGLSPVISIEQKTTNKNPRSTVGTITEIYDFMRLLFARASDAYSYETGKKMVRFSE
                     EQIIDNIIQRFEGKRIALLAPLIRGRKGHYRELFEQVRKQGYLKVRVDGDVLDLVPRM
                     QVDRYKVHDIEIVVDRFRVDETDRYRITQSVQQALKAGKQFIMVEDSDAGKVYNYSKK
                     LMCPDSGISYEEPSPNTFSFNSPYGACLHCKGLGLVYEPDLSLIIPDDGKTINNGGLL
                     PLGEYRDNTLFQQARSISRKYKFTLSTPIKDIPKKGLNMILFGNEKGEALWHEDDVAD
                     MTGDKWYTLEDGGLTFLLMRCFSESVSENMRRWAEDFMGKTICDVCQGTRLKKESLFF
                     RVHEKNISELADMDVRSLVDWFQHVEEGMDERQQAIARDVLKEIRARLGFIDNVGLGY
                     LSLNRATRTLSGGESQRIRLATQIGSQLTGITYILDEPSIGLHQRDNLRLIEALQELR
                     DTGNTVLVVEHDKEMMLQSDYIIDLGPGAGIH"
BASE COUNT          783 a          671 c          753 g          646 t
ORIGIN      
        1 gacaaatact ttgagaactg ccgggaaaag ggatacgatg atgcgctgac caaagatgtc
       61 tggcatcaga tagagagttt tgccggctac agttttgcca aggggcattc tgcctcttat
      121 gcggtagaga gttatcagag tctcttcctc aagacctatt acccgctgga gttcatggtg
      181 ggtgtcatca ataacttcgg tggtttctac agcacagaat tgtatgtgca tgaagcccgt
      241 atggcaggtg gtcgtatcga agcgccctgt gtgaacaaca gcctgtatgc taccaccatc
      301 ggcgggaaga ccatccacct ggggtttcag catatcaaat cactggaaca caagatcgcc
      361 accggcatac cggaagaacg gctgcagcac ggtccttttc tcagcctgga tgattttctg
      421 caccgggtat ccatcaccct cgagcaactg gagatcctca tcctcatcaa cgcctttcgt
      481 tttaccggaa tcagcaaaca ggagctgctc attgaagcct atatgcgcat gcacaagacc
      541 aggcgcacgg ttccctctct gcagctgttc gccgcccaac ccgaccaacc gtatcagttt
      601 ccctccctgc atagtgatat gctggaagac gcctgggatc aggtcaagct gctgggcttt
      661 ccgctggcat cgcccttcac cctgatagac ccggaagtgc tggaagatcc ggatctggta
      721 tgcgcccgtc agctgccgta tctgaacggt caggaagtgg aggtagtagc atactacgtg
      781 acccggaagg tgacctacac catcaaggga gaggtcatgt tcttcggcac cttcattgac
      841 cgcaacggcg agtgggtgga caccgtacac tttccggatg tggcaaagca gtaccgtttc
      901 aggggcaagg cctgttaccg tatcagaggc aaggtgacgg aagaattcgg cacctggagc
      961 atagaggcgc attacctgga aatgataccc atgttattac ctaaaggcat ctgacgaaac
     1021 gggatgaaac aagagatgcc ccgcctaaaa gccgtacctt tgcagaactt ttcaaaacgc
     1081 agtccgttgt cagaaaaagt ggttagaaaa aagcaggcac cgctgccgga atcaagtccg
     1141 gacattcctt atctggaggt aagaggtgcc cgtgaacaca atctgcgcaa tatagatgta
     1201 agcatacccc gcaacagcct ggtggtcatt accggagtaa gcggaagtgg taaaagttct
     1261 cttgcatttg atacgatcta tgcagaagga caacgccggt acatggaaac gttcagttcc
     1321 tatgcacagc agtttgttgg caacatggag cggccggaag tggaagagat caccggtttg
     1381 agtccggtca tttccataga acagaagacc accaacaaga acccgcgttc aactgttggc
     1441 accatcacag agatctatga cttcatgcga ctgctgtttg cccgggcttc agatgcatac
     1501 agttatgaga cgggaaaaaa aatggtcagg ttctcagaag aacagatcat tgataatatc
     1561 atacagcgat ttgaggggaa acgtattgca ctgctggcgc ctttgattcg cgggcggaaa
     1621 ggtcattacc gcgaactgtt tgaacaggta cgcaaacaag gctatctgaa ggtaagggta
     1681 gatggcgacg tgcttgacct ggtaccacgc atgcaggtgg acaggtacaa agtgcatgat
     1741 attgagatcg tggtggaccg attccgggtg gatgaaacag acagataccg cataacccag
     1801 agtgtacaac aggcgctgaa agccgggaag cagttcatca tggtggaaga tagtgatgcg
     1861 gggaaggtgt acaactacag caagaaactg atgtgcccgg acagcgggat atcctatgaa
     1921 gaaccttcgc cgaatacatt ctccttcaat tcaccttacg gtgcctgtct gcattgcaaa
     1981 ggactgggac tggtatatga accagacctc agtctgatca ttcccgacga tggcaagacc
     2041 atcaacaacg gcggactcct gccattagga gaataccgcg acaatactct tttccagcag
     2101 gcacgaagca tatccagaaa gtataaattc actttgtcca cacctatcaa agacatcccg
     2161 aaaaaaggat tgaatatgat cctgtttggg aatgagaaag gggaagcttt gtggcatgag
     2221 gatgatgtgg cagatatgac cggcgacaag tggtacacac tggaagatgg tggtctgacc
     2281 tttctgctca tgagatgttt cagtgaatct gtttcagaaa atatgcgtcg ctgggccgaa
     2341 gacttcatgg gcaagaccat ctgtgatgta tgtcagggca cacgactcaa gaaggaatca
     2401 ttgttcttcc gggtacatga aaagaacatc tctgaactgg ctgatatgga tgttcgctca
     2461 ctcgtagatt ggtttcaaca tgtagaggaa ggcatggatg aaagacaaca ggccattgcc
     2521 cgtgatgtac tgaaagaaat acgggcacgt cttggattca ttgataatgt agggctgggt
     2581 tatctgtctc tcaacagggc cacccgaact ttgtcaggtg gtgagtcgca gcgcatacgt
     2641 cttgccacac agataggatc tcagctaacc ggtatcacct atattctgga tgagccatcc
     2701 atagggcttc atcagcgaga caaccttcgt ttaatagaag cgctgcagga actcagagac
     2761 accggaaata cagttcttgt ggtggaacac gacaaggaaa tgatgctgca atcagattat
     2821 atcatagacc ttgggccagg tgcaggtatt cat
//
LOCUS       DAOMAX010000325         2853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7499_length_2853_cov_2.953181, whole genome shotgun sequence.
ACCESSION   DAOMAX010000325 DAOMAX010000000
VERSION     DAOMAX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7499_length_2853_cov_2.953181"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..508)
                     /locus_tag="PK742_12280"
     CDS             complement(53..508)
                     /locus_tag="PK742_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77413.1"
                     /translation="MAEPKQGYDVVSAHMLKEDFIQRGLKDDRFPFRVSAAFSLLALF
                     LLLLASLAADRNEMLMAAGGTDLLLYLLANAIFSLGVHEFERFVKRSVLAYLIHVVIL
                     SGLLYLFLGGLPEDAREVLPVYGALIMSFFLSVGLVVLIRQVLSILREK"
     gene            555..1604
                     /locus_tag="PK742_12285"
     CDS             555..1604
                     /locus_tag="PK742_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HQU77414.1"
                     /translation="MAVSASKQQKIDAFDINGIGQANGHLFGLPFGFEDAEVVILPVP
                     WEVTVSYRPGTAGAPEAILEASYQVDLYDADLPDAWKRGVYMLSVSPEWGKTSRQLRV
                     KAAEHIRLMESGHADGSGQTVEEVNDASNRLRKWVFEQCSEVLEQGKLPALLGGDHST
                     PLGLMQALSLKYPSFGILQIDAHADLRKAYEGFTYSHASIMYNALQIEQVKGLVQVGL
                     RDVCQEEMDLIRSDDRIHAFFHQDIRRSLYEGHSWKQVCATVIDRLPEYVYISFDIDG
                     LDPKLCPNTGTPVAGGFEYAEVVFLIQELVHAGKKVIGFDLVEVGPSDDEWDANVGAR
                     LLYKLSNLMLKSQGK"
     gene            1601..2161
                     /locus_tag="PK742_12290"
     CDS             1601..2161
                     /locus_tag="PK742_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQU77415.1"
                     /translation="MRYLLPVLLLLTACTHQRPDSLDEISLSTTEGKTWNGTDLSSHA
                     ATVFIFFAPQCPLSENYCLQTNVLQAQYLSEGVQFFAVIPGALFSSEEINDFLQRYQL
                     DVPVLLDPDKYLCDVLGATITPEAFLTDPAGQTLYSGAIDNWAVDLGKKREVVTEFYL
                     RDAIDAILQKQPVPISKTTAVGCYIE"
     gene            2158..>2853
                     /locus_tag="PK742_12295"
     CDS             2158..>2853
                     /locus_tag="PK742_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77416.1"
                     /translation="MKRYLVIAVIPVILLIACGKEKKEVTFADVAPVIRENCMPCHRP
                     GSAGPFSLISYDDMVRKAKTIVRVTQSGFMPPWPADPEYTHFAGERRLTEDQKELIRK
                     WVEAGTPLGDTTLLSPPPHYPDGSMLGEPDLVVPFTDTIILPGDNDDHFFLVKVPFTT
                     DQQRFIRTIEYIPGNKKLAHHMNANLIRYADGAKTNVFDGEKVIATDLTLTAESVNDR
                     MKNKNDDGSYPVLV"
BASE COUNT          782 a          705 c          687 g          679 t
ORIGIN      
        1 cttgtcaaag gtgccaccgg tcacgaaaat ggaaatgctt ttatccaccc gcttactttt
       61 cacgtaaaat tgataaaacc tgacgaatca atactaccag tccgaccgaa aggaaaaaac
      121 tcatgatcag tgcaccatac accggcagta cttccctggc atcttccggc aatccaccca
      181 gaaataggta caaaagtcct gataaaatga ccacatgaat cagataggcc agaacagagc
      241 gttttacgaa acgttcaaat tcatgaacac ccagtgagaa aatggcattt gccagaagat
      301 agagcagcag atcggtgcca ccggctgcca tcagcatctc atttctgtct gctgccagtg
      361 aagccagcag cagcagaaac agagccagta aactgaatgc agccgatacc cggaaaggga
      421 atctgtcatc cttgagtcca cgctgaataa aatcttcctt tagcatgtgt gcagatacaa
      481 catcataccc ctgtttgggt tcagccaaat gtactccatt tcagggagca tgacagcatc
      541 cttatctttg tcatatggcg gtaagtgcaa gcaagcaaca aaagatagat gccttcgata
      601 tcaatggcat cgggcaggct aacggacatt tgttcggtct gccatttgga tttgaagacg
      661 ctgaggtggt catacttcct gtgccctggg aagtgactgt ttcctacaga cccggaactg
      721 ccggtgcacc agaggccatt ctcgaagctt cttatcaggt ggacctgtat gatgctgacc
      781 tgcctgatgc ctggaaaaga ggggtctata tgctgtccgt atcgccagag tggggaaaga
      841 cctccaggca acttcgtgtg aaggcagcag aacatatcag actgatggag tctggtcatg
      901 ctgacggttc aggtcaaacc gttgaagagg tcaacgatgc cagcaaccgg ctgcgcaagt
      961 gggtctttga acaatgcagt gaagtactgg agcagggtaa gctgcctgct ttgctgggtg
     1021 gtgatcacag cactccactt ggactgatgc aagccctgtc tttaaaatat ccgtcattcg
     1081 gcatcttaca gattgacgca catgctgacc ttcgcaaggc atatgaaggc ttcacctatt
     1141 cacatgcaag tatcatgtac aatgcgctgc agatcgaaca ggtaaaaggt ctggttcagg
     1201 tagggctccg cgatgtttgt caggaagaaa tggacctgat cagatcagat gatcgtatcc
     1261 atgcattctt ccaccaggac attcgccgat cactctatga aggacattcc tggaaacagg
     1321 tctgcgcaac cgttatcgat cgactgccgg agtatgtcta tatcagcttc gatattgatg
     1381 gcctggatcc taaactgtgt ccgaataccg gtactccggt ggccggcggt ttcgaatatg
     1441 cagaggtggt ctttctgatc caggaactgg tacatgcagg caaaaaggtc attggttttg
     1501 atctggttga agtgggtcct tcagacgatg aatgggatgc caatgtaggc gccagattac
     1561 tatataaatt atcaaatctg atgctgaaaa gccaggggaa atgaggtact tacttccagt
     1621 cctgctgctg cttacggcct gcacccatca aaggcctgac agcctggatg agatcagcct
     1681 gtccacaacg gaaggaaaaa catggaacgg tacagatctc tcttcgcatg cagccactgt
     1741 gtttattttt tttgctccgc aatgcccgct cagtgaaaat tactgcctgc aaaccaatgt
     1801 actgcaggca cagtaccttt ctgaaggggt gcagttcttc gctgtgatac caggtgctct
     1861 gttcagcagc gaagagatca atgattttct gcaacgttat caactggatg tacctgttct
     1921 gctcgatccc gataaatacc tgtgtgacgt gctgggcgca accatcacac ctgaagcctt
     1981 tctgaccgac cctgccggac aaacattata ctccggagcc attgacaact gggcggttga
     2041 tctgggaaag aaacgggaag tggtgaccga attttatctt cgtgatgcca tagatgccat
     2101 cttgcagaag caaccggttc ccatttccaa aaccactgca gttggctgct atatagaatg
     2161 aaaagatacc tggtcatagc tgttattccg gtcattcttt tgatagcctg cggaaaggaa
     2221 aagaaagaag tgacctttgc cgatgtggcc ccggtcatca gagaaaactg catgccttgc
     2281 caccgacccg gatctgcagg acctttcagc ctcatctcct atgatgatat ggtcaggaaa
     2341 gccaagacca tcgttcgggt aacacaatca ggtttcatgc ctccgtggcc ggccgatcct
     2401 gagtacaccc actttgcagg agaacgccgg ctcaccgaag atcagaaaga actgatcaga
     2461 aaatgggtgg aagccggtac accactgggc gatacgaccc tcctcagccc tcccccccac
     2521 tacccggatg gatctatgtt aggtgaacca gacctggtgg tgccgttcac agacaccatc
     2581 atccttccgg gcgataatga tgatcatttt tttctggtaa aggtgccttt cacaacagat
     2641 cagcaacgat tcatacgaac catagaatac ataccgggga acaagaaact ggcccatcac
     2701 atgaatgcca atcttatccg ctatgcagat ggagccaaaa caaatgtatt tgatggcgaa
     2761 aaagtgatcg caactgatct gacactgaca gctgaatctg ttaacgatcg catgaaaaac
     2821 aaaaatgacg acggctccta tccggtgctt gta
//
LOCUS       DAOMAX010000326         2848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7516_length_2848_cov_4.581812, whole genome shotgun sequence.
ACCESSION   DAOMAX010000326 DAOMAX010000000
VERSION     DAOMAX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7516_length_2848_cov_4.581812"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK742_12300"
     CDS             <1..186
                     /locus_tag="PK742_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQU77417.1"
                     /translation="LTEKESAVLRDLVEGFSYKMIAENQGISYHTVNFHVRNIYRKLH
                     VHSLGEAVSKAIREKLI"
     gene            284..>2848
                     /locus_tag="PK742_12305"
     CDS             284..>2848
                     /locus_tag="PK742_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HQU77418.1"
                     /translation="MRLPILLSAILIAAPILSYATTIAETESNNTSGTANAVAVNDSV
                     TGSVLGADLDFDWFSFTTAEDGIIRINYAITGSGHANIYLLDNDGTTILSSATVVPAG
                     TTASFQYNKAAAGDYFMRIQYYSAATDCSYELVPEVITNGYGQDAEPNDVYTDALVLA
                     ENSSADGHLGYRYNGGALDQIDWYQVTTTEDGNLNVTLDIEPSSYFNLSIYDSNGVTS
                     LGSASASDLVSVTRNGVAAGVYYIRVHYYSTTYYGGYTLTNSLTSTGIPNDAEPNDVS
                     TQALVLPENGSTQGHIGYRYNGGSYDEDDWYELTTTSDGDVTVSLAYSVGMYHRLYLY
                     AADGVTNLASTGGYDSISVTKAGLAAGTFLVRVEHYSTTYYSPYTLYNAVAMTEYDND
                     AEPNDVYTDAMVMDENSTMYGHIGHRYDVGGFDEDDWYQVVTTQDGTLTITAENNTGQ
                     YHTLTLYDSNGTTSLGSNSAYTTQTVVRKGLAAGTYYIRMSHYSTTYFSGYSLTNLVT
                     PPAEANDMEDNDSIPVAQPMLTNSSAEGHIGFRYNGATYDEYDYYELVLNQPGDLSAT
                     LTFDPVMYHRLYLKSSSGTNLDSDEAYGECQVSETGLDAGTYYILVEHYSTTYHSGYT
                     LTNNYCPDTVVIMAEGITTFCEGESVLLFTEDHHLSYLWSDGSTTETNAADITGGYSL
                     TIDNGDGCVRTSNIVDIEVEPNPLAVIDLDGPAEFCEGGSVTLSISSDAPDAYLWSTG
                     ESTPTIEVTTSGDYSVELFKGTCSAISDPVAITVWENPVASVTPLSATTLCEGEAVTL
                     DAGAASEYLWSTGETTSSIDVDATGSYSVTITDVNGCTDISNAVDVTVNPIPVATIT"
BASE COUNT          795 a          626 c          666 g          761 t
ORIGIN      
        1 cttacagaaa aagagagtgc tgtattacgc gatctggtag aaggattcag ttacaagatg
       61 attgctgaaa accagggcat cagttaccat accgtcaatt tccatgtgcg caacatctac
      121 aggaaactcc atgtgcacag cctaggtgag gcagtgtcca aagccatacg ggaaaagttg
      181 atttaacacg tttttaaccg gtacctgcaa gaattagtag tatggcagga gcatggatac
      241 cgatagattt gggattctgt ttatgaaacc caaaatgaaa aacatgcgcc taccaatcct
      301 gctgtctgcg atactgatcg cagcaccgat attatcttat gccactacta ttgctgagac
      361 cgaatccaat aacacttcag gtactgctaa tgcagtggct gtgaatgaca gcgtgaccgg
      421 ctctgtgctt ggggcagatc tggactttga ctggttctca ttcacaaccg ctgaagatgg
      481 tatcatccgc attaattacg ctattactgg atcgggtcat gctaatatat acctgctgga
      541 caatgacggt acaacaatac tgtccagtgc aactgtagtt ccggctggaa ctacggcatc
      601 atttcagtac aataaggcgg cggctggcga ctacttcatg cgaattcagt actacagtgc
      661 cgcaacagat tgcagctatg aactggtgcc cgaggtcata acgaatggtt atggtcagga
      721 tgctgaacca aatgatgtat atacagatgc tttggtgttg gcagaaaata gttcagcaga
      781 cggccattta ggttaccgct ataatggtgg cgcactggat cagatagact ggtaccaggt
      841 caccacgaca gaagatggta atctgaatgt gacgctggat attgaaccca gttcctattt
      901 taatctatcc atttatgatt caaatggagt gacttcgctg ggctcagctt cagcttctga
      961 tctggttagc gttaccagaa atggtgtagc tgccggtgtg tattatatcc gtgtacacta
     1021 ttattcaact acttactacg gaggatatac actcacaaat tcacttacat ccacaggtat
     1081 ccctaacgat gcagaaccaa atgatgtctc tactcaagca cttgttctgc ctgagaatgg
     1141 aagcacacag gggcatattg gttatcggta taatggagga agctatgacg aagatgactg
     1201 gtatgaactt acaaccacca gcgatggtga tgtgacagtt tcattagctt attctgttgg
     1261 catgtaccac cggctatacc tgtatgctgc agacggagtg accaatctgg cttcaactgg
     1321 gggttatgat tcgatctctg tgacaaaggc cggacttgct gccggtacat tccttgtacg
     1381 ggtagagcac tactcaacca cttattacag cccttataca ttatacaatg cagtggctat
     1441 gacagaatat gacaatgacg ctgagcctaa tgatgtatat acagatgcca tggttatgga
     1501 tgaaaacagt accatgtatg gacatatcgg tcaccggtat gatgtgggtg gatttgatga
     1561 ggatgactgg tatcaggtag taacgaccca ggacggtaca cttaccatta ctgctgaaaa
     1621 taatacaggg cagtaccata cccttacact ttacgattcc aacggaacaa cgagcctggg
     1681 ttccaatagt gcttatacaa cccaaacggt tgttcggaaa ggactggctg caggtacata
     1741 ctatattcgc atgtcacact attcaactac ctatttcagt ggttacagct tgaccaacct
     1801 ggtcacccca ccggctgaag caaatgacat ggaagacaat gattcaatac cggtagcaca
     1861 gcctatgctt accaattcat ctgcggaagg tcatatagga ttccgctata acggagctac
     1921 ctatgatgaa tacgattact atgagttagt gctaaatcaa ccaggagact tgtctgctac
     1981 cctgaccttt gatcctgtca tgtatcacag gctatatctc aagagtagct ccggaaccaa
     2041 ccttgacagt gatgaagcct atggcgagtg tcaggtttca gaaaccgggt tggatgccgg
     2101 aacatattat atattggttg aacattattc aactacctat cacagcggat atacccttac
     2161 caacaattac tgtccggata ccgtggtcat tatggctgaa ggaatcacta ccttctgtga
     2221 aggagaatct gtgttattgt ttacggaaga tcatcacctg tcatacttat ggagtgatgg
     2281 ctctactaca gaaaccaacg ctgcagatat tacaggtggt tattcactta ctattgataa
     2341 tggcgatggc tgtgtgcgta cctccaatat cgttgacata gaagtagagc ctaacccctt
     2401 ggctgtgatc gatctggatg gtcctgcaga attctgcgaa ggtggtagtg tgacactgag
     2461 tatcagttct gatgccccgg atgcatatct ctggagtact ggtgagtcca ctcctaccat
     2521 tgaagtaacc acttccggcg attatagtgt ggagctgttc aaaggaactt gctctgccat
     2581 ttcagacccg gttgcgatca ctgtgtggga gaatccagtt gcttcagtaa ctccgcttag
     2641 tgctactacg ctttgtgagg gagaagcggt aacactggat gcaggagctg cttctgaata
     2701 tctgtggagt accggcgaaa caacctcttc cattgatgtg gatgcaacag gcagttactc
     2761 agtaaccata acagacgtaa acggatgtac agatatatct aatgcagtgg atgtgaccgt
     2821 gaaccccata cctgtggcta ctatcaca
//
LOCUS       DAOMAX010000327         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7560_length_2839_cov_3.345187, whole genome shotgun sequence.
ACCESSION   DAOMAX010000327 DAOMAX010000000
VERSION     DAOMAX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7560_length_2839_cov_3.345187"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PK742_12310"
     CDS             <1..443
                     /locus_tag="PK742_12310"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HQU77419.1"
                     /translation="ESFADFYRDSRLMPLYKQAAERGVMHVNAAFDKLCNRLEELIPA
                     ENPSLVHGDLWSGNVLFDAQGTPVLIDPAVAYSHRECDIAMSMMFGGFDQSFYDAYDQ
                     NHPLVPGWEARIPLFQLYPLLVHVILFGGSYVRQAEEVIRKFGG"
     gene            607..1263
                     /locus_tag="PK742_12315"
     CDS             607..1263
                     /locus_tag="PK742_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77420.1"
                     /translation="MNRTLFIFAVTACIAKSSLAQSHQPGTLSFQASVDLGVHGTVYH
                     SEDGGIVLANDTSSAATTLFSLSAQYQLNNWLSVGVNGRTGVYHEDPENAEEAGNHIR
                     TLSADLRLYPVNKENFNWYLGVDYGIAALEINRNYVFIVPFTAEYDLSGSHLGLFTGF
                     NWYFLDFAGIHMNLAYSRHAFDLKHYYLSGQEQDLGSSINTLDALGVHFQAGLSFRIN
                     "
     gene            1440..2249
                     /locus_tag="PK742_12320"
     CDS             1440..2249
                     /locus_tag="PK742_12320"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQU77421.1"
                     /translation="MNKALIEQAWEDRSLLQQEETKQAILEVISQLDSGALRVAEPTS
                     DGWQVNDWVKKAVIMYFPIAKMETIEVGPFEFHDKIPLKKDYAAKGVRVVPHAIARYG
                     AYVSKGVIMMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPVQ
                     ASPVIIEDNCFIGSRCIVVEGVHIEKEAVLGANVVITQSTRIIDVSGAEPIEHRGRVP
                     AGSVVIPGSYTKSFPAGDYQVSCALIIGKRKESTDKKTSLNSALREYNVAV"
     gene            2246..>2839
                     /locus_tag="PK742_12325"
     CDS             2246..>2839
                     /locus_tag="PK742_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HQU77422.1"
                     /translation="MKPLAFDIARSAFDQLHWEDQELRIIRTVAETADELQLETYLVG
                     GYVRDKILDRKVVDMDFVCVGDGIILAEKVAQKLKGKPEVSIFKNFGTAHIRFGKLDL
                     EFVGARKESYQHHSRKPDVEPGTLQDDQLRRDFTINALAIALNKERYGQLIDPFNGMQ
                     DLHDGRIRTPQEPAATFSDDPLRMMRAIRFASQLGFHI"
BASE COUNT          716 a          688 c          751 g          684 t
ORIGIN      
        1 cagaaagctt tgcagacttc taccgcgaca gccgcctgat gccgctttac aaacaggctg
       61 ccgaacgtgg agtgatgcat gtaaatgctg ccttcgataa gctatgcaac agactggaag
      121 aactcattcc cgctgagaac ccctccctcg tacatggcga tctctggtca ggaaatgtgt
      181 tgttcgatgc gcagggaaca cctgtgctca tagacccggc ggtggcctat tcgcaccgtg
      241 agtgcgacat tgccatgagt atgatgttcg gaggattcga tcagagcttt tatgatgcct
      301 atgaccaaaa ccatccgctt gttccaggat gggaagcacg cattcccctg tttcagctct
      361 atccgttgct ggtgcatgtc attctttttg gaggtagcta tgtcaggcag gctgaagaag
      421 tgatccggaa gttcggtggt tagtcgttga tcgtttttcg tgattcgttg atcgttatgt
      481 ttaggacaga aagcctttcc tgatcatctg aaaaaaatat ggctgaacca tttggtatga
      541 taacaatcgc aaaacgaatg acgaataacg aataacaaat aacgaataac cctagtatat
      601 ttgaagatga acagaacact cttcattttt gctgttactg catgtatagc caaatcttct
      661 ttggcccaat cacaccagcc cggaacctta tcttttcagg cgagtgtgga cctcggtgtg
      721 catggcacgg tttatcattc agaggacggt ggtatcgtgc tggccaatga caccagcagt
      781 gcagccacga ccctctttag tctgagtgcc cagtatcaac tgaataactg gttgtctgtc
      841 ggggtgaacg gcaggaccgg tgtgtatcat gaagaccctg agaatgcaga agaagcaggt
      901 aaccacattc gaacgttgtc agcagacctg cgactgtatc ctgtcaacaa agaaaatttc
      961 aactggtacc tgggtgtcga ttatggcatc gctgcactgg agatcaatcg caactatgtg
     1021 ttcattgtac cgtttacagc agagtatgac ctgagcggct ctcacctggg attgttcacc
     1081 ggtttcaact ggtatttcct ggattttgcc ggcattcata tgaacctcgc ttacagcagg
     1141 catgcctttg atctgaaaca ctattacctg agcggacagg aacaagacct tggttcttcc
     1201 atcaatacgc tggatgcgct gggcgtgcat ttccaggcag ggttgtcgtt caggataaat
     1261 tagtattggg tccctcccga tagctatcgg gagctatcgg gatgagtagt gggtagtggg
     1321 aattttgtta tgtttctaca cactcacacc caaactccta cactcaaata tttacacctc
     1381 cccttcctga ctgtggactt cagactccgg actgaacgca gttccgctat ctttgcacca
     1441 tgaacaaagc cctgatagag caagcgtggg aagacagaag tttactgcag caggaagaga
     1501 caaaacaggc cattctggag gtgatttcac agctggacag tggagccctg cgggtggcag
     1561 aacccacttc cgatggctgg caggtgaatg actgggtgaa gaaagcagtg atcatgtact
     1621 tccccatcgc caaaatggag accatagaag tgggcccttt tgagttccac gataagatac
     1681 cgctgaagaa ggattatgcc gctaaaggtg ttcgggtggt acctcatgcc attgcccgct
     1741 atggtgctta tgtgagcaaa ggggtcatca tgatgccttc ctatgtcaac atcggtgctt
     1801 atgtggatga aggtactatg gttgatacct gggccaccgt gggaagttgt gcacagatcg
     1861 gcaagaacgt acacctgagc ggtggtgtgg gaattggtgg tgtgctggaa cccgtacagg
     1921 ccagtccggt catcatcgaa gacaactgct tcatcggctc cagatgcatt gtggtggaag
     1981 gtgtacatat tgaaaaggaa gccgtgctgg gcgctaatgt ggtcatcacc cagagcaccc
     2041 gcatcattga tgtgagtggt gcagaaccga tagagcatcg cggacgggta ccggctggca
     2101 gcgtggtcat tcccggcagc tatacgaagt cttttcccgc cggcgattac caggtgagct
     2161 gtgcacttat cataggcaag cgcaaggaat cgaccgacaa gaaaacaagc cttaactccg
     2221 cactcagaga atacaacgta gccgtttgaa acccctcgct ttcgatatag cacggtcggc
     2281 ttttgaccag ctgcactggg aagatcagga gttgcgcatc atcagaaccg ttgctgaaac
     2341 ggccgatgaa ctgcagctcg agacctacct ggtgggcgga tatgtcagag acaagatcct
     2401 cgaccggaag gtggtggata tggactttgt ctgtgtggga gatgggataa tcctggccga
     2461 aaaagtagca cagaaactca aaggcaaacc ggaggtcagc atctttaaga acttcggcac
     2521 cgctcatatt cgcttcggaa aactcgacct ggagttcgtg ggagccagga aagaatctta
     2581 ccagcatcac agtcgcaaac cggatgtcga acccggtacc ttacaggacg atcagctgcg
     2641 ccgcgatttc accatcaacg ctctggccat tgcgttgaac aaggaacggt acggacagct
     2701 catcgatccc ttcaacggca tgcaggacct gcacgatggt cgcatacgca ccccgcagga
     2761 accggcagca actttcagcg acgatccgct gcgtatgatg cgggccatac gttttgcttc
     2821 gcaactgggt tttcatatc
//
LOCUS       DAOMAX010000328         2814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7673_length_2814_cov_4.362813, whole genome shotgun sequence.
ACCESSION   DAOMAX010000328 DAOMAX010000000
VERSION     DAOMAX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7673_length_2814_cov_4.362813"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PK742_12330"
     CDS             complement(<1..1087)
                     /locus_tag="PK742_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQU77423.1"
                     /translation="MSGFRFSQFHAPDRSVFDQLFELFRELLVYTSGDVTEALDWLNQ
                     LDQQYHITTDEYGMGDFIQELKDRGYLQDEQPGMAGMQMTGKMEQSIRQSSLEQIFGK
                     LKRSNSGNHRTNYSGQGDELSADRRHFQFGDTPDQIDFSDSLRNAYINHGIDDFHLME
                     NDLVVAEKEFKAQTSTVLMIDVSHSMILYGEDRITPAKKVGMALSEMITTRYPKDTLD
                     ILAFGNDAWPIEIKDLPYLQVGPYHTNTVAGLELAMDLLRRRKNPNKQIFMITDGKPT
                     CIIRNGKHYKNSFGLDRIIVNKTLNLAAHCRRMGIRITTFMIARDPYLQRFVEEFTRV
                     NQGKAFYTSLQGLGEYIFEDFERNRRRK"
     gene            complement(1089..2150)
                     /locus_tag="PK742_12335"
     CDS             complement(1089..2150)
                     /locus_tag="PK742_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQU77424.1"
                     /translation="MQYQLLGKSGVRVSEICLGTMTFGKEWGWGADEKDSREVFDAYL
                     ESGGNFIDTANRYTEGTSEQFLGKLINGSGKRDDLVIATKFSLVTRSGDGINRSGNHR
                     KNIVQSVEGSLKRLGLDYIDLYYLHAWDYTTRIEEVLRALDDLVRQGKILYTGISDTP
                     AWIVSRGDAIAQLQGWTPFSALQVEYSMLQRSTERELIPMAAALDMAVLAWAPIAGGA
                     LTGKYLENNSAREGDDGNRRLKENSSRLNERARSIAAELVAVAGETKYLPHQIAIAWV
                     KKQGEHIIPLVGARTAAQLRENLAAADVMLSDEQWSRLDAVSRIDPGFPHEFLQTDGV
                     KQILYGNAADKLTTHRKGF"
     gene            2221..>2814
                     /locus_tag="PK742_12340"
     CDS             2221..>2814
                     /locus_tag="PK742_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HQU77425.1"
                     /translation="MKKIAVALVLAFSFYQAEAQNYADSVAFRKIFNEVLTNGECYDW
                     LHDLCKDVGHRLSGSPQADMAVRWGEAVMREAGFDSVWLQPVMVPKWVRGQKEYAEIL
                     GVGQVDVLALGGSIATPTDGITAQVVYAETFDDLEKLGREKIAGRIVFFNEYFDPTNI
                     NTFESYGQCVPYRWKGASEAAKYGAAAVVIRSVGSAYD"
BASE COUNT          640 a          704 c          677 g          793 t
ORIGIN      
        1 ccttcctcct tcggttgcgt tcaaaatctt caaagatgta ttctcccagt ccctgcaagc
       61 tggtataaaa tgctttgccc tggtttaccc gggtgaactc ctctacaaat cgctgcagat
      121 aagggtcgcg ggcgatcatg aaagtggtga tgcgaatacc catgcgccgg caatgcgccg
      181 ccaggttgag ggttttgttg acgatgatgc ggtctagccc gaagctgttc ttatagtgtt
      241 ttccgtttcg tatgatacag gtgggcttac catcggtgat catgaagatc tgtttgtttg
      301 gattctttct tctccgcaac aggtccatcg ccagttccag tccggctact gtattggtat
      361 ggtatggccc cacctggaga taaggcagat cttttatttc gatgggccag gcatcgtttc
      421 caaaagccag aatatccaaa gtatctttcg gataacgggt cgtgatcatc tcgctgagag
      481 ccatgcctac cttcttcgcc ggtgtgatgc ggtcttcacc atacaagatc atagagtgag
      541 acacatcgat catcagcacc gtggaggttt gcgccttgaa ttccttttca gctacgacca
      601 ggtcgttctc catcaggtga aaatcatcga tgccgtggtt gatgtaggcg ttgcgcaaac
      661 tatcactgaa atcgatctgg tcaggggtat cgccaaactg aaaatgacga cggtcagcac
      721 tgagttcatc tccctgacct gaatagttcg tgcgatggtt tccgctgttc gatcttttca
      781 gtttgccgaa gatctgttcg aggctggact gccttatgga ctgttccatt tttccggtca
      841 tctgcatacc agccatgccg ggttgttcat cctgcaggta accccggtct ttcagttcct
      901 ggatgaaatc gcccatacca tattcatctg tggtaatgtg gtattgctga tccagctggt
      961 tgagccagtc cagtgcttcc gtaacatcgc cactggtata taccagcagt tctctgaaca
     1021 attcgaagag ctgatcgaat accgaacggt ccggtgcatg gaactgagag aaccgaaagc
     1081 ctgacatatc agaatccttt cctgtgtgtg gttaatttgt cagcagcatt gccgtacagg
     1141 atctgtttca ccccgtcggt ttgcagaaac tcatgtggga atccggggtc gatcctgctt
     1201 actgcatcca gccggctcca ttgttcatct gaaagcatca cgtctgctgc tgccaggttt
     1261 tcacgaagtt gcgctgcagt tctggctcct accagaggaa tgatatgttc accttgtttt
     1321 ttgacccacg ctatagcgat ctggtgcgga agatactttg tttctccggc cacagctacc
     1381 agttcagcag ctatggatct tgccctttca ttaagccgtg aactgttctc tttcagccga
     1441 cggtttccgt catcgccttc ccgggcgctg ttgttttcca gatacttgcc ggtcagtgca
     1501 ccacctgcta tgggtgccca ggccagtaca gccatgtcta acgctgcagc cataggaatc
     1561 agttctcttt ctgttgatct ctgcaacatg gaatactcca cctgcaatgc agaaaagggc
     1621 gtccatccct gcagttgagc gatggcatct ccgcggctca ctatccaggc cggtgtatca
     1681 ctgataccgg tgtacaggat cttaccctgc ctgaccagat catcgagcgc acgcaacact
     1741 tcttctattc tggttgtata atcccaggca tggaggtagt acagatctat gtaatcaagc
     1801 cccagacgtt tcaatgatcc ttccacactc tgcacaatat tcttccgatg attcccactt
     1861 cggttgatgc catcgcctga cctggttacc aacgaaaatt tagtggcgat taccagatca
     1921 tcccgtttgc ctgatccatt gatcagtttt cccagaaatt gctcgcttgt tccctctgta
     1981 tagcggttgg cggtatcaat aaaatttccg cctgattcca gatatgcatc aaagacctct
     2041 ctgctgtctt tttcatcagc accccagccc cattcctttc caaaggtcat ggtacccaga
     2101 cagatctctg acacacgcac accacttttt cccaataatt gatattgcat gatagtctga
     2161 actgttaaac gtagtgaatg gttcagccaa atttcgaaaa ctatttgtta tttcggtctc
     2221 atgaaaaaaa tcgcagttgc tctcgtcctg gcattttctt tctatcaggc agaagcacag
     2281 aactatgctg attctgttgc cttcaggaaa atttttaacg aagtcctgac caacggcgaa
     2341 tgctatgact ggctgcatga cctgtgcaag gatgtagggc accgactgag tggttcgccg
     2401 caggccgaca tggctgtcag gtggggagaa gctgtgatgc gggaagctgg attcgattct
     2461 gtctggcttc aaccggtgat ggtgccgaag tgggttcgcg gacagaaaga atatgctgag
     2521 atcctgggtg ttgggcaggt agatgtgctg gcattgggtg gttctattgc cactcctacc
     2581 gacggtatta ccgcacaggt ggtgtatgca gaaaccttcg atgatctgga aaaacttgga
     2641 agagaaaaaa tagcaggcag gatcgtcttc ttcaatgaat attttgaccc gaccaatatc
     2701 aatacatttg aatcatacgg acaatgtgtt ccttaccggt ggaagggtgc ttcagaagct
     2761 gcaaaatatg gtgcagcagc agtggtgatc agaagtgtag gatctgctta tgac
//
LOCUS       DAOMAX010000329         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7680_length_2813_cov_4.068165, whole genome shotgun sequence.
ACCESSION   DAOMAX010000329 DAOMAX010000000
VERSION     DAOMAX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7680_length_2813_cov_4.068165"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PK742_12345"
     CDS             <1..955
                     /locus_tag="PK742_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQU77426.1"
                     /translation="LPAMGIASEVLSVHSRKPIFGYTAMIYSLLAIAVLSFIVWAHHM
                     FMTGMNPFIGSIFVIFTLIIAVPSAVKVFNWLTTLWRGNIKLTPPMMFAIGFVSLFIS
                     GGVTGIFLGNSSIDVELHDTYFVVAHFHLVMGVAAFFGMFAGIYHWFPKMFGRHMNNT
                     LSYIHFWGTIVCAYLIFFPMHWEYMLPRRYPTHAGFDTFGMFAQLNEFITIAAMASFA
                     FQLIFVFNFFYSIWYGRKLKEQNPYHATTLEWTTPIRPGHGNWPGDIPEVHRWAYDYS
                     KHGRDFIPQTEPLSQYEIEHGEHGDEMDSDVIAHENDDHQS"
     gene            957..1847
                     /gene="cyoE"
                     /locus_tag="PK742_12350"
     CDS             957..1847
                     /gene="cyoE"
                     /locus_tag="PK742_12350"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQU77427.1"
                     /translation="MSRTSAITDSRWMRLKARLADYVLLVKFRLSFTVVFSAGIGYMF
                     AGGDIAYFSLFLLAGFLITGASNALNEILETNSDKLMSRTAVRPLPAGRMQAGEAILA
                     SGFMGVIGILLMWIFFSPLAALLGAISLLTYSFIYTPLKKVSPIAVWVGAFPGAMPPL
                     IGWAAATGEIGTMAVLLFTIQFMWQFPHFWSIAWVAFEDYAKADFYLLPASTGRDKRS
                     AVQVVLYTVLLIGVSTLPFLLGYTGIVSLIITLAAGLLFLLQAIRLWQTCSDKMARQL
                     MFGSFLYLPVVLIALFADKM"
     gene            1851..2447
                     /locus_tag="PK742_12355"
     CDS             1851..2447
                     /locus_tag="PK742_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQU77428.1"
                     /translation="MSQSISHKRFIIHPYKFNMWLAIVAMIMMFAAFTSAYVVKKADF
                     RYWVDVPFPQMFTFSTITILLSSVFMHLSLVTFRRNQVSLHRLFMLLTLVAGTSFLAM
                     QIMGWQELYDNGIKLNGNVAGSFLYVISGAHFLHVAGGVIGLLVFSVFAFTRFRNPVD
                     TLVENIHPYKQIGLELMATYWHFVDVLWLYLFFFLQYS"
     gene            2459..>2813
                     /locus_tag="PK742_12360"
     CDS             2459..>2813
                     /locus_tag="PK742_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase subunit III"
                     /protein_id="HQU77429.1"
                     /translation="MEATVQSNARELWSGGDSPFKVSYGKIMMWYFLISDTFSFAGLL
                     ISYGAIRLSQPAHDEANPLSWPDPNHVFNAVPFLHGHYPLVFVTFMTFTLIFSSVTMV
                     RAVQEGHAMNRKGVLF"
BASE COUNT          633 a          660 c          673 g          847 t
ORIGIN      
        1 cctgcctgcc atgggtatcg cttcagaagt tctgtccgtt cattcacgta aacccatctt
       61 cggatatact gcgatgatct attcactgct ggctattgca gtactgtcat tcatcgtctg
      121 ggcgcaccat atgttcatga ccggtatgaa tccgttcatc gggtccatct tcgtgatctt
      181 tacattgatc attgctgtac catcggcagt caaggtattc aactggctaa ccacattatg
      241 gcgtggtaat atcaaactga cacctccgat gatgttcgct atcggatttg tttccctgtt
      301 catttcaggt ggtgtaacag gtatctttct gggtaactct tccattgatg tggagttgca
      361 tgatacctat ttcgtggtag cacacttcca cctggtaatg ggtgtggctg cattctttgg
      421 aatgtttgcc ggaatctatc actggttccc taaaatgttt ggtcgccata tgaataatac
      481 ccttagttac atacacttct ggggtaccat cgtatgcgcc tacctgatct tcttccccat
      541 gcactgggaa tatatgctgc cacgtcgtta tccgacccat gcaggttttg atacattcgg
      601 catgtttgca caactgaatg agttcattac cattgctgca atggcatctt ttgccttcca
      661 gctgatcttc gtgtttaact tcttctacag catctggtac ggaaggaaac tgaaagaaca
      721 gaatccttat catgccacta ctctggaatg gaccacaccg atccgcccgg gtcacggtaa
      781 ctggcctggc gatattcctg aagtgcaccg ctgggcatat gattacagca agcatgggcg
      841 tgatttcata cctcaaacag aaccgttgtc tcagtatgag atcgagcatg gcgaacatgg
      901 cgatgaaatg gacagcgatg tgatcgcaca cgaaaacgac gatcatcagt cataagatga
      961 gcagaacttc tgccataaca gattcccgtt ggatgcggct aaaagcccgg ctggcagact
     1021 atgtgctgtt ggtcaagttc aggctaagct tcactgtggt attttctgca ggcatcgggt
     1081 atatgtttgc aggcggagat atcgcttact tctcgttgtt tttacttgcc ggatttctta
     1141 tcacaggagc ctccaatgcc ctgaatgaga tcctggaaac caacagcgat aaactgatga
     1201 gcagaaccgc tgtgagacca ttgcctgccg gcagaatgca ggcgggagaa gcaattctgg
     1261 catccggatt catgggcgtt atcggaatac tgctgatgtg gatcttcttt tcaccacttg
     1321 ctgcgcttct tggtgcgatc tctttgctca cctattcgtt tatctacaca ccattgaaga
     1381 aggtcagtcc cattgccgta tgggttggcg cttttccggg agccatgcca ccgcttatcg
     1441 gatgggcggc tgcaaccgga gagatcggca ctatggcggt tctcttgttt accatccagt
     1501 tcatgtggca gtttccgcat ttctggagca tcgcatgggt ggcgtttgaa gactacgcca
     1561 aagctgactt ttatttattg cctgcatcca ccggccgcga caagcgttcg gccgttcagg
     1621 tggtactata tacagtctta ctgatcgggg tgagtaccct gcctttcctg ttgggttata
     1681 caggtattgt ttcactgatc atcacccttg cagcaggttt gttgtttcta ttgcaggcaa
     1741 tacgcctatg gcaaacctgt tcagacaaga tggcccgaca gctaatgttt ggttcattcc
     1801 tgtatttgcc ggtggtattg atcgcattgt ttgccgacaa aatgtaacac atgagtcaga
     1861 gcatcagtca taagcggttt atcattcatc catataagtt caatatgtgg ctggccatcg
     1921 tggctatgat catgatgttt gccgcattta ccagtgctta tgtggtcaaa aaagcagact
     1981 tccggtattg ggtggacgtg cctttccctc aaatgttcac tttcagcacc atcaccattc
     2041 tgctaagcag tgtattcatg cacctgtcac tggtaacttt cagaagaaat caggtgagtt
     2101 tgcaccggtt gttcatgctg ctaacgctgg ttgcaggaac ttcttttctg gctatgcaga
     2161 tcatgggctg gcaggaatta tatgacaatg gcataaagct gaatggcaat gtagcggggt
     2221 cattcctgta tgttatatca ggagcccatt tcctgcatgt ggccggtggt gtgatcggac
     2281 tgctggtttt ctctgtattt gcgttcaccc gattcaggaa tccggttgat acgctggtgg
     2341 agaatattca tccttataag cagataggtc tggagctgat ggccacctac tggcattttg
     2401 tggatgtatt atggttgtat cttttcttct ttttacaata tagctaaaca cgaaaccttt
     2461 ggaagcaaca gtacaaagca atgcacggga actttggagc ggaggcgatt ctcccttcaa
     2521 ggtgagctat ggcaagatca tgatgtggta tttcctcatc tctgacacat tttcatttgc
     2581 cggtttgctg atttcatatg gcgctattcg cctgagtcag ccggcgcatg atgaagcgaa
     2641 cccgctcagc tggcctgacc ctaaccatgt tttcaatgca gtaccttttc tgcatgggca
     2701 ttatccgctg gtattcgtca cattcatgac attcaccctg atctttagtt ccgttactat
     2761 ggtacgtgcg gtacaggagg gacatgctat gaaccgaaaa ggcgttttgt tct
//
LOCUS       DAOMAX010000330         2810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7697_length_2810_cov_3.269328, whole genome shotgun sequence.
ACCESSION   DAOMAX010000330 DAOMAX010000000
VERSION     DAOMAX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7697_length_2810_cov_3.269328"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PK742_12365"
     CDS             complement(<1..1116)
                     /locus_tag="PK742_12365"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HQU77430.1"
                     /translation="MKIYDAPFIRAADRYTIEQGISSADLMERAARACSDQILKEHPD
                     IQQVLVICGTGNNGGDGLCIARHFLELSMPVKVLIHGHRESGTEDLSHHLRRFESAGG
                     ICESYSGRLPDIPQDTIVIDALFGTGINRPVEGTAATLIEQLNESGKTILSVDMPSGL
                     PANGIPEGTVIHAHHTYSFQWPKPAFFLPENAGYTGSWSILDIGLRADFPGHEHAWGE
                     WVDSSFVLPFLKPRKTFSHKGNYGHVMLLAGSKDMPGAANLCSFACIRAGAGRVTLAD
                     DGREETRDEIMHLHRNKLVPALESGQYNVFAAGPGLGRTPSAHTLLKQCLESFRHPVL
                     LDADALNLLADDPSLTMLLPAGSILTPHPGEFRRLAGD"
     gene            complement(1113..1892)
                     /locus_tag="PK742_12370"
     CDS             complement(1113..1892)
                     /locus_tag="PK742_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQU77431.1"
                     /translation="MLESGITKISLLPVRSEPRQQAELVTQLLFGEMYTIHEKQGEWL
                     RIVTHSDQYSGFINVSQYSPLDARTARDLENSDKSVVLDLVHSITENDRKFPVSIGSI
                     LYHFDGVTARIGKDKYHFSGKTIPSGQGFGQPDMIIKLAMKLLHAPYLWGGRGPFGID
                     CSGFVQLVYRCAGVELPRDAWQQAATGKTLHFIHEARAGDLAFFSNEEGRIIHVGMLM
                     GDGTIIHASGMVRIDTIDHYGIFRKDIRKYSHQLRIIKRIL"
     gene            2069..>2810
                     /locus_tag="PK742_12375"
     CDS             2069..>2810
                     /locus_tag="PK742_12375"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77432.1"
                     /translation="MSDQDAMRLKIQVVLLLLLSVTTVNLRAQVVAAFDADTTLGCGV
                     LELSMINLSTDATTYSWQVYNSSGTLVASSTLTNPSFFLTTPDDYTVTLTATGPGGSD
                     TFTADDFASVVTPPNAVIEVDPTSGCPPVTITAENLSTPGSLGSIESFYWIITGAGSL
                     PATDVINYTFETSGTYSVFLFVQDEAGCSDFAEVLVIPDPAPAIDFTADETISCEEPF
                     TVNFDNLSTGDYMPFTYLWDFGDGTTSTL"
BASE COUNT          642 a          734 c          701 g          733 t
ORIGIN      
        1 atctccggca agtcggcgaa attcaccggg gtggggggtg agaatactac cggcaggcaa
       61 caacatggtc agtgacggat catccgccag cagattcagt gcatctgcgt ccagcagtac
      121 cggatgtctg aaggattcca ggcattgctt cagcaacgtg tgggcagaag gagtgcgacc
      181 cagtccggga ccggctgcaa acacattgta ctgaccactt tccagagccg gcaccagttt
      241 gtttctgtgc aggtgcatga tctcatcacg ggtttcttcc cgaccatcgt cagcaagggt
      301 tacccttcct gctcctgcac gtatgcaggc aaaggaacac agattggctg caccgggcat
      361 atctttacta ccggccagca gcatgacatg accatagttg cctttatggc tgaaagtttt
      421 tcttggcttc agaaagggga gtacgaaact gctgtccacc cattcacccc aggcatgttc
      481 atgccccgga aaatctgctc ttagcccaat atccagtatg ctccagcttc cggtatatcc
      541 ggcattttcc ggcagaaaaa atgcgggctt aggccactga aaactgtagg tgtgatgcgc
      601 atggatcacc gtaccttcag ggataccatt tgctggcaga ccggagggca tatcaacgga
      661 gagaatggtt tttccacttt cattcagttg ttctatcagg gtagccgctg ttccttctac
      721 gggtctgttg ataccggttc caaaaagggc atcgatcacg atcgtatctt ggggtatatc
      781 gggtagtctg cctgaatagc tttcacagat accaccggca gattcaaacc ggcgcaggtg
      841 gtgagataag tcttctgttc cagattcgcg gtggccgtga ataagcactt tgaccggcat
      901 actcagctct aaaaagtgcc gtgcaatgca taagccatct ccgccattgt ttcctgtacc
      961 gcatattacc agtacctgct gtatgtcagg atgttctttc agaatttggt cactgcaggc
     1021 tcttgcagct ctttccatca ggtcagcaga actgatcccc tgttctatgg tataacggtc
     1081 ggctgcacga atgaaagggg catcgtagat cttcatagta ttcgtttgat gatccgcaac
     1141 tggtgagaat acttgcggat atctttcctg aatataccat aatgatcaat ggtgtcaatg
     1201 cgcaccatgc cagaggcgtg gatgatggta ccatcgccca tcagcatgcc cacatgtatg
     1261 atgcgcccct cttcattgga aaagaaagcc agatcgccgg cacgggcttc atgaatgaag
     1321 tgcagggtct ttccggttgc tgcctgctgc caggcatctc tgggcagttc aacaccggcg
     1381 cacctgtaca ccagttgtac gaaaccggaa cagtcaatgc cgaatggccc ccggcctccc
     1441 cataaataag gggcgtgcag cagcttcatg gccagtttta tgatcatatc tggttgcccg
     1501 aaaccctggc cggaagggat ggttttaccg ctgaaatggt atttatcctt tccgatccgg
     1561 gccgttaccc cgtcaaaatg gtacaaaata ctgccaatac tgacagggaa tttccggtca
     1621 ttttcggtga tgctgtgcac caggtccagt acaactgatt tgtcactatt ctccagatct
     1681 ctggcagttc ttgcatccaa agggctgtat tgggatacat taatgaagcc ggagtactga
     1741 tcagaatgtg tcactattcg cagccattct ccttgttttt cgtgtatagt gtacatttca
     1801 ccgaacagga gttgtgtcac aagttcggct tgctgacgag gctcgctgcg cacaggcagg
     1861 aggctaatct ttgtgattcc ggattccagc atggtaggga acgagccaaa ttacaattgc
     1921 cggctgttat atctgtgcct tttccaacac gctattgtga acatccaaat gagactttcc
     1981 ttttggcctg ctcacttttg tccctacttt taaaacggat agcacacact gatggtcaca
     2041 catagatgca agatcaggtg aatttggtgt gtctgaccag gacgccatgc ggttgaagat
     2101 acaggtcgtt ttgttactgc tgctgtcggt cacgacagtg aacctgcgtg cacaggtggt
     2161 cgcggctttt gatgccgata ccacgcttgg ctgcggggtg ctggaattat ccatgatcaa
     2221 cctgtcaacg gatgctacca cctactcctg gcaggtgtac aattcatcag gcacactggt
     2281 ggccagcagt acacttacca atccttcttt ctttctgacc actccggacg actatacggt
     2341 cacactgact gctacagggc ccggagggtc tgatacattt acggccgacg attttgcatc
     2401 tgtggttaca ccacctaatg cggttattga ggtggatcct acctctggct gcccgccggt
     2461 taccatcact gctgagaacc tcagtacgcc cggatcgctg ggcagcattg agagttttta
     2521 ctggatcata accggagcgg ggtcgcttcc cgccactgat gtcatcaact acacgtttga
     2581 aacctcaggc acctattcgg tattcctgtt tgtgcaggat gaagccggat gcagcgattt
     2641 tgccgaagta ctggtgatac ctgacccagc acccgccatt gactttacag ccgatgagac
     2701 catctcctgt gaagagcctt tcacggtcaa ctttgataac ctgagcaccg gcgattatat
     2761 gccgttcacc tacctctggg attttggcga tggtactacc tctacccttg
//
LOCUS       DAOMAX010000331         2808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7707_length_2808_cov_3.401380, whole genome shotgun sequence.
ACCESSION   DAOMAX010000331 DAOMAX010000000
VERSION     DAOMAX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7707_length_2808_cov_3.401380"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PK742_12380"
     CDS             complement(<1..502)
                     /locus_tag="PK742_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77433.1"
                     /translation="MKTLYLRPMRGIRILIAPFFLLLAGNSAGWAQFGTNNAGARTLA
                     MGNTSLTLTDAYAAANNQAAMAFAEGPAFGVSALQYFSLSELTSLWGCAVLPAAGGAF
                     GLSVHHDGSQTFRQTQAGVGYGRKLGENIAVGVQLDLIHTGVTDLGNGNTFTAEAALL
                     YKPSPEL"
     gene            596..>1554
                     /locus_tag="PK742_12385"
     CDS             596..>1554
                     /locus_tag="PK742_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQU77434.1"
                     /translation="MDVQTMMIAVPILGLIALLFTYIRTRWVASQEVGGDKMALIAKN
                     ISDGAMAFLRAEYRVLAIFVVLVAALLAFAGYREEDSSPLVGASFIVGALCSALAGFI
                     GMKVATKANVRTTNAARTSLGKALEVAFTGGSVMGLGVVGLGVLGLGSLFLIYGQMIG
                     MDSHADVNLVITIITGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHP
                     LNPATIADNVGDNVGDVAGMGADLFESYVGSIIGTMVLGAALLSGATVAGAEWTDGFN
                     GLSGIMLPLVLAGVGILTSIMGTFFVRVKEGGNPQKALNAGEL"
     assembly_gap    1555..1654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1655..2791
                     /locus_tag="PK742_12390"
     CDS             <1655..2791
                     /locus_tag="PK742_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQU77435.1"
                     /translation="GVFLAVIFGLFAGLGIGYITEFYTGTGTRPVKGIARQSLTGSAT
                     NIISGLGVGMQSTAIPILILAVAIIGAFHFAGLYGIAIAAVGMLSNTGIQLAVDAYGP
                     ISDNAGGIAEMAELPKEVRGRTDKLDAVGNTTAAIGKGFAIGSAALTALALFAAFMQT
                     AGITTIDISKPIIMAGLLIGGMLPFLFSSMAMGAVGRAAMDMIQEVRRQFADIPELNK
                     ALAVMRKNEGKEMNEWDAADKATFDAANGKAEYGKCVEISTKASIREMVLPGLVAVLS
                     PVLVGFLGGAEMLGGLLAGVTVSGVLMAIFQSNAGGAWDNAKKMFEEGVDINGKTYYK
                     GSEPHKAAVVGDTVGDPFKDTSGPSLNILIKLMSVVSLVIASLL"
BASE COUNT          595 a          636 c          755 g          722 t
ORIGIN      
        1 tcaactcagg cgaaggtttg tacagcaaag cagcctcagc agtaaatgta ttgccgtttc
       61 ccagatcggt cactccggta tggatcagat ccagctgcac accaacagcg atgttttctc
      121 ccagtttgcg tccgtatcct acaccagcct gtgtttgcct gaaagtctga ctgccatcgt
      181 gatgaaccga taaaccaaaa gcaccccctg ctgccggtaa tacggcacaa ccccaaagtg
      241 aagtgagttc tgacaaactg aaatactgca gtgctgaaac accaaaagca ggaccttcag
      301 caaatgccat cgctgcctgg ttattggctg cagcataagc atcagtaagg gtcagcgatg
      361 tgttgcccat ggccagtgtt cttgctccgg cattattggt gccaaattga gcccaacctg
      421 cagagttgcc tgcaagcagc agaaaaaatg gtgcaattag gatacgaatg ccccgcatgg
      481 ggcgaagata cagggttttc aagggctgac gattctgctg tggtgaaata tttatgaaat
      541 attgtggctc tgaatgccta tttttgcgcc tcgaaaaatc caaaaaatta ccgacatgga
      601 tgttcaaact atgatgatcg ctgttcccat tctgggattg atagcgctgc tcttcaccta
      661 tatcagaacc cgctgggtag cgagtcagga agtaggtggc gacaaaatgg ccctgatcgc
      721 aaaaaatatc agcgatggtg ccatggcttt cctgcgtgcc gagtaccgtg tactggccat
      781 ttttgttgta ttggttgctg ctctgctggc ttttgcaggc taccgcgaag aagactcttc
      841 tccgctggtt ggtgcctcct ttattgtagg agctctttgt tcagcccttg ccggattcat
      901 cggtatgaaa gtggctacca aagcaaacgt gcgcaccacc aatgcagccc gcaccagtct
      961 gggtaaagcc ctggaagttg cctttaccgg aggctcagtt atggggctcg gcgttgtcgg
     1021 actcggtgta ctgggtctgg gttcgctctt tctgatctac ggtcagatga tcgggatgga
     1081 tagccatgca gatgtgaacc tggtgatcac catcattaca ggattcagct tcggtgcctc
     1141 ctctattgca ctttttgccc gtgtgggtgg tggtatctat accaaggcag ccgatgtggg
     1201 tgccgacctg gtgggtaagg tggaagccgg tattccggaa gatcacccgc tgaaccctgc
     1261 taccattgct gataacgttg gagataacgt aggagatgtg gccggtatgg gtgccgacct
     1321 ttttgaatct tatgtcggat cgatcattgg taccatggta ctgggtgcag ctctgctgag
     1381 cggtgctact gtagcaggtg ccgaatggac cgatggtttt aacggactgt caggtatcat
     1441 gctgccgctc gtgctggcag gtgtgggcat cctgacctcc atcatgggta cattctttgt
     1501 tcgtgtgaaa gaaggtggaa acccccagaa agccctgaac gcaggcgagc tcatnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtgta ttcctggctg tgatcttcgg
     1681 tctgtttgcc ggtctgggta tcggttatat cacagaattc tataccggaa cgggtacccg
     1741 tccggtcaaa ggcattgctc gtcagtcgct gacaggttct gctaccaata tcatttccgg
     1801 tctgggagta gggatgcagt ccactgccat tccgatcctg atcctggctg tagccatcat
     1861 tggtgccttc cactttgcag gtctgtatgg tatcgccatt gctgcggtgg gtatgttgtc
     1921 aaacaccggt atccagctgg cggtagatgc ttatggtcct atctcagaca acgccggtgg
     1981 tatcgctgaa atggcagaat tgcctaaaga ggttcgtgga cgcaccgata agctggatgc
     2041 cgtaggtaac acaaccgctg ccattggtaa aggctttgct attggatcag ctgcgttgac
     2101 cgctttggca ctgtttgctg cttttatgca gactgcaggt attaccacaa ttgatatttc
     2161 caaacccatc atcatggcag gtctgttgat cggtggtatg ctgccgtttt tgttctcttc
     2221 catggcgatg ggagcggttg gtcgtgcagc catggacatg atccaggagg ttcgccgtca
     2281 gtttgccgat attcctgaac tgaacaaggc actggcagta atgcgcaaga atgaaggcaa
     2341 ggaaatgaat gagtgggatg ctgcagacaa ggccacattc gatgctgcca acggtaaggc
     2401 tgagtatggc aagtgtgtag agatctctac caaggcttct atccgtgaga tggtacttcc
     2461 cggtctggtg gctgtattgt cgccggtact ggttggcttc ctggggggtg ccgaaatgct
     2521 gggtggtctt ctggcgggtg tgaccgtttc aggggtattg atggccatct tccagtccaa
     2581 tgcgggtggt gcctgggata atgccaagaa aatgttcgag gaaggtgttg atatcaatgg
     2641 caagacctat tacaaaggtt ctgagcctca caaggcagcc gttgtgggtg atactgttgg
     2701 ggatcctttc aaggatactt ccggaccttc cctgaatatt ctgatcaagc tgatgtcagt
     2761 agtatcgctg gttattgcca gcctgctctg atagcaccat agtattgt
//
LOCUS       DAOMAX010000332         2805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7723_length_2805_cov_3.761455, whole genome shotgun sequence.
ACCESSION   DAOMAX010000332 DAOMAX010000000
VERSION     DAOMAX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7723_length_2805_cov_3.761455"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK742_12395"
     CDS             <1..278
                     /locus_tag="PK742_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HQU77436.1"
                     /translation="ERLKVDHEHAKQIGAILENQSWVDTCLPVESNIVIFQLAAQYSA
                     DDLISYLQAQDIHAFHIGNNQVRLVFHMDVSADMTGQIVRMLQSYMQ"
     gene            391..963
                     /locus_tag="PK742_12400"
     CDS             391..963
                     /locus_tag="PK742_12400"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQU77437.1"
                     /translation="MNKLISFFLCLMISGGVVLSQTNYTLSSGSSKMKITGGSNVHEW
                     DETVNKLSGDARITIGSDVFDIISLNFKADVKSIESSKGSIMDDKTYEALKASKYPYI
                     TFELDKVLKASPVSNGYWLNTQGYLTIAGSRKLVKLDVKAIYTDASSIAFEGTKTFNM
                     SEFGIDPPTAMMGSMKVKDEVKIQFKVYYK"
     gene            994..2409
                     /locus_tag="PK742_12405"
     CDS             994..2409
                     /locus_tag="PK742_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77438.1"
                     /translation="MRNLSNILKSSLVVTMLLSTGLLFAQQPGISHFRGYDKNAVNMF
                     ETSPQDVVPFTGIKVRVGGGFTQVFQALSHENVIDTVAASLIDGNGDGVDDRKLYNLS
                     PGFDLAEANLNLDVQLADGIRLSLETYLSTRHHSETWVKGGYIQVDKLPFGDDNSFFN
                     ENMFVRVGHMEINYGDAHFRRSDAGNTLQNPFMEGYILDAFTTEIGGELYYNANNILV
                     MLGMTSGEIKGNVLAESALDPASNLDPLALDTNKRSPSILAKLGYDNQMNDDLRIRLT
                     GSMYYTASSSRNTLYGGDRSGSDYHLVMENTAATADTKFTSGRYNPGFTDNVMAIMIN
                     PFVKFQGLEVFITYEMATGRSLTELAPTEGAEDIDVNRAASQIAADLIYRFGANEDFY
                     VGGRYNTMTADYRDFNDNAEPILSQGTINRIAGVFGWFVTDNVLAKAEFVTQSYEGFP
                     ETSIFNGGKFSGIVLSGSVGF"
     gene            2479..>2805
                     /locus_tag="PK742_12410"
     CDS             2479..>2805
                     /locus_tag="PK742_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77439.1"
                     /translation="MRLAITCLFIQLTILVHAQVSKASYTITLKQTERLELQGTSNAV
                     DFTCGYPARRNTAPLSFVWSEGWFHPAPVISLKVRQLDCGGAGINNDMRKTLQEESYP
                     EILLQPT"
BASE COUNT          772 a          665 c          633 g          735 t
ORIGIN      
        1 tggagcgcct gaaagtggat catgaacacg caaaacagat cggggctatt ctggaaaacc
       61 aaagctgggt agatacctgt cttccggttg aatccaatat tgtgatcttt caattagcag
      121 cgcagtattc tgccgatgat ctcatcagtt acctccaggc gcaggatatt catgcctttc
      181 atataggcaa caaccaggtc agactggtat tccatatgga tgtgtcagct gacatgaccg
      241 ggcaaattgt ccggatgctc cagtcatata tgcagtgaat gctgccagga gcagaaaaaa
      301 tggtgacatt aatcacttct ggcagcaagt gattgtcagt atcaggcata taccctccgg
      361 atacctttgt tccagcaaag taaaaagatc atgaacaagt tgataagttt cttcctgtgc
      421 ctgatgatca gcggcggggt tgttttaagt caaactaatt acacactgtc ttccggttct
      481 tccaaaatga agatcacagg aggatcaaat gtccatgaat gggatgaaac cgtaaacaaa
      541 ctcagcggag atgcccgcat caccattggc tcagatgtat ttgatatcat atctctgaat
      601 tttaaagccg acgtcaaatc cattgaaagt tccaaaggtt ctatcatgga tgacaagaca
      661 tatgaagcac tgaaagcctc caaatatccc tatatcacct ttgagctgga taaggttctg
      721 aaagcctctc ctgtatcaaa cggttactgg ctgaacaccc agggctatct gaccatagca
      781 ggcagcagaa aactggtcaa gctggatgtg aaagctatct atacagatgc atcatccatt
      841 gcctttgaag gaacaaagac tttcaacatg agtgaatttg gcattgaccc gccaaccgcc
      901 atgatgggat ccatgaaggt aaaagacgaa gtgaagatcc aatttaaggt ctattataaa
      961 taaccaaacc attactcact aaaatttcat gctatgagaa acctaagcaa tattctaaaa
     1021 agctcgctgg tagtgaccat gttgctctct actggtttgt tgtttgccca acaacccggc
     1081 atcagccact tccgtggcta cgataagaat gccgtaaaca tgtttgaaac ctctccacag
     1141 gatgttgttc cttttacagg catcaaagtg cgtgtaggtg gtggtttcac ccaggtattt
     1201 caggcactca gccacgaaaa cgtgatcgac actgtagctg cttcgcttat tgatggaaac
     1261 ggcgatggtg tagatgatcg taagttgtac aatctgtctc ccggttttga tctggcagaa
     1321 gccaatttaa atctggacgt gcagcttgct gatggtatcc gcctgtcact ggaaacatac
     1381 ctgtcaacca ggcaccactc tgaaacatgg gtaaaaggtg gttacattca ggttgacaaa
     1441 cttccttttg gcgatgacaa cagctttttc aatgagaata tgtttgttcg tgtaggccat
     1501 atggagatca attatggtga tgctcacttc cgtcgcagcg atgccggtaa caccctccag
     1561 aatccgttca tggaaggtta tatcctggat gcattcacaa ctgagatcgg tggtgaattg
     1621 tactacaatg ccaacaacat cctcgtgatg cttggtatga ccagcggcga gatcaaagga
     1681 aacgtattgg cagaatctgc tctggaccct gccagtaacc tggatcctct tgcacttgat
     1741 accaacaagc gctctccttc catcctggct aaactgggct atgacaacca gatgaatgat
     1801 gacctgcgca tccgcctgac cggtagcatg tactatactg caagttccag caggaatacc
     1861 ctgtatggtg gcgaccgcag tggttcagac tatcacctgg tgatggagaa caccgcagct
     1921 actgctgata ccaaattcac ttccggccgc tacaaccccg gcttcactga caacgtcatg
     1981 gccatcatga tcaacccgtt cgtgaagttc cagggtcttg aagtattcat tacttatgaa
     2041 atggcaaccg gccgcagcct gaccgaactt gctcctactg aaggtgccga agatatcgat
     2101 gtgaacaggg ccgcttctca gatagcagct gacctgatct accggtttgg tgcaaatgaa
     2161 gatttctatg ttggcggacg ttacaacacc atgacagcag actatcgcga cttcaatgac
     2221 aatgcagaac ctatcctgtc tcagggtacc atcaatcgca ttgccggtgt attcggatgg
     2281 tttgttactg ataatgttct tgccaaggca gaattcgtta ctcaatccta tgaaggtttc
     2341 cctgaaactt ccatcttcaa cggtggcaaa ttcagcggaa ttgtactcag tggatctgta
     2401 ggtttctgac gtttggaatg atagtgaact cccgggaaca ttcccgggag tttttattta
     2461 tagatacatt agttcctcat gcgactcgca atcacctgcc tattcattca gctgaccatc
     2521 ctggttcatg ctcaggtgag caaagcatca tataccatca cattaaagca aactgaacgc
     2581 ctggaactac aaggcaccag caatgccgtt gattttacct gcggataccc ggccaggcgt
     2641 aatactgctc cgttgtcttt tgtctggagt gaaggctggt ttcatcctgc tcctgtaata
     2701 tctctgaaag tcagacagct ggattgcggt ggtgcaggca tcaataatga tatgcgcaag
     2761 accctgcagg aagagagcta tcccgagata ctgctccagc ctacc
//
LOCUS       DAOMAX010000333         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7853_length_2777_cov_4.514695, whole genome shotgun sequence.
ACCESSION   DAOMAX010000333 DAOMAX010000000
VERSION     DAOMAX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_2777_cov_4.514695"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1347)
                     /locus_tag="PK742_12415"
     CDS             complement(16..1347)
                     /locus_tag="PK742_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU77440.1"
                     /translation="MRTAFAALSILLMTQLQAQELFKDPPEWSRNANIYEVNIRQYTP
                     EGTFKAFQAHLPRLQRMGVDILWLMPVQPIGVENRKGSLGSYYSISDYTTTNPEFGSM
                     EDLKELVEQAHQKGMKVILDWVANHTSFDNVWVSRHPDWYTRDKNGNLTPPVEDWSDV
                     ADLNFDNTDMRRAMIDAMKFWVNEAGVDGFRCDVAMMVPNDFWKQCVSALQEVKPDIF
                     MLAEAEGPKFHASGFDMTYAWDIHNIMNKVASGEKTADELEAFVQEDIVKYPEGAYRM
                     LFTTNHDKNSWEGTEFERMGDGAQAFYVLCATLPGVPLIYSGQEMGLNRALRFFDKDT
                     IGFSDNPPYESFFRSLNNLKKDNQAIWNGDNGGTLYTVHVDNAVWVYARQKGDDKVLV
                     IVNLSPDVHNVKLKSDVFAGKYRDTFTGDKVKLGSKYKGELKPWSYVVLEN"
     gene            complement(1404..>2777)
                     /locus_tag="PK742_12420"
     CDS             complement(1404..>2777)
                     /locus_tag="PK742_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU77441.1"
                     /translation="NMGFNTLWLSPVMQNPHVAYQEYPEPHRWFSGYHGYWPISLSQV
                     DDRFGTNTQYKALIDSLHKENMNIVFDYIANHVHAEHPLWTEHPDWFTPLELPDGRKN
                     LRLWDEQRLSTWFEPYMPSLDHSQPQVAEASADSAMWWLRNYDIDGFRHDATKHIETE
                     FWRLLTEKIHQESLRPGGYPVYQIGETFGSRELIGSYLGSGLLNAQFDFNLFFDARNI
                     LAEDEGSFRDLSLSLHLSFDYYGYHHLMGNITGNHDLARFISYAGGALSLSEDDKAAG
                     WERAIEVQDPRGYKKLELLHVFNMTIPGIPIVYYGDEIGMPGAGDPDNRRMMRFEGLT
                     QPEQHVKLTVRQLAKMRASHMALLYGDFHELYVDDQVWVYARTYLDDQVIVCLNKGYN
                     AATVDLELPAYLNGFIGSGKQDGQNQQQEQQNQQQGESTEGGMSILGNTLQISVPPYG
                     YQILFSH"
BASE COUNT          658 a          703 c          673 g          743 t
ORIGIN      
        1 attagctcat tgatatcagt tctccagcac cacgtaactc cagggtttca attctccttt
       61 atacttgctg ccgagtttga ccttatcgcc ggtgaaagta tcgcggtact tgccggcaaa
      121 gacatcactc ttaagcttca cgttgtgtac gtctggtgag aggttgacaa tgaccagtac
      181 cttatcatcc cctttctgac gggcatagac ccaaacggca ttgtccacat gtacggtata
      241 aagtgtgccg ccgttgtcgc cgttccagat ggcctggttg tctttcttca gattgttgag
      301 tgaacggaag aatgattcat acggcggatt atcactgaag ccaatggtgt ccttatcgaa
      361 gaagcgcagg gcccggttca agcccatttc ctgtccgctg tagatcaaag gtacacccgg
      421 tagtgtagca cataacacat agaatgcctg agcaccatcg cccatacgtt caaattctgt
      481 cccctcccag gaattcttgt cgtggttggt cgtgaacagc atacggtagg caccttcagg
      541 atacttaacg atatcttcct gcacaaatgc ctccagttca tcggcagtct tttcgccgct
      601 ggccacctta ttcataatgt tgtgaatatc ccacgcatag gtcatgtcaa aaccactggc
      661 gtggaattta ggcccttcgg cttcagccag catgaagatg tcaggcttga cctcctgtaa
      721 agccgatacg cattgtttcc agaaatcatt tggcaccatc atggccacat cacacctgaa
      781 tccgtccaca ccggcctcat tgacccagaa tttcatagcg tcgatcatgg cacgacgcat
      841 gtcggtattg tcgaaattca gatcggctac atcactccag tcttctaccg gaggggtgag
      901 gtttccgttc ttatctctgg tgtaccagtc aggatggcgg gagacccaaa cgttatcaaa
      961 ggaagtatga ttggccaccc agtccagaat gaccttcatg cctttctggt gtgcctgttc
     1021 caccagttcc ttcaggtcct ccatactgcc gaactccggg ttggtggtgg tgtaatcgct
     1081 gatggagtaa taactgccca gactgccctt tctgttttct acacctatcg gttgaacggg
     1141 catcagccat agaatatcta cgcccatacg ttgcaagcga ggcaggtgtg cctggaatgc
     1201 tttgaaggtt ccttcaggag tatattggcg gatgttgacc tcatagatgt tggcattgcg
     1261 gctccactcg ggcgggtctt taaacagttc ctgtgcttgc aactgggtca tcaataaaat
     1321 ggatagggct gcaaaagcag ttctcatggt atgtgtggtt aagtgaaaca aattattggg
     1381 tttcagtcaa agatagacag gaatcagtgg ctgaacagga tctgatatcc atatggtggt
     1441 acacttatct gcagagtgtt tcccaaaata gacattccac cttccgtgct ttcgccctgt
     1501 tgctgatttt gttgctcttg ctgctggttt tgaccgtcct gtttaccaga accaatgaac
     1561 ccgttcaggt aagcgggtag ctccaggtca acagttgccg cattataccc tttattgaga
     1621 caaacgatca cctgatcatc caggtaggta cgggcatata cccatacctg atcatctaca
     1681 tacaactcat ggaagtcgcc atacaacaat gccatatgtg aagcacgcat tttagccagc
     1741 tgcctcacag tgagcttaac atgttgttcc ggctgggtga gcccttcgaa acgcatcatt
     1801 ctgcggttat ccggatcgcc tgcaccgggc atgccgatct catctccgta atatacgatc
     1861 ggaatacccg gaatggtcat attgaataca tgcagtaatt ccagcttctt ataacctctt
     1921 ggatcctgca cttcaatagc cctttcccat cccgcagcct tatcatcttc agataaagac
     1981 aaggccccac cggcgtatga aatgaaccgg gccaggtcgt ggttaccggt aatgttaccc
     2041 atgaggtgat ggtatccgta gtagtcaaaa ctgaggtgaa ggcttagtga cagatcgcgg
     2101 aaggaacctt cgtcttcggc caggatattc cgggcatcaa agaacagatt gaagtcgaac
     2161 tgagcattga gcaaaccgct gcccagatag ctgccaatga gctcccggct gccaaaggtc
     2221 tcgccgatct ggtacaccgg gtaacctccg ggtctcagtg attcctgatg gatcttttct
     2281 gtaagtaatc tccagaattc tgtttcaata tgtttggtgg catcatgacg aaaaccgtct
     2341 atatcgtaat tgcgcaacca ccacatagcg ctgtctgcac tggcttcggc tacctgcggc
     2401 tggctgtggt ccaggctggg catatagggt tcgaaccagg tgctgaggcg ttgttcatcc
     2461 cagagtcgaa gattctttct gccatccggc agttccagag gagtgaacca gtcggggtgt
     2521 tcggtccaca gcggatgttc tgcatgcaca tggttagcaa tatagtcaaa aacaatattc
     2581 atgttttctt tgtgcaagct atcgatcaga gctttgtact gggtgttggt tccaaagcgg
     2641 tcgtccacct gcgacaggct gatgggccag taaccgtgat agccggagaa ccagcggtgg
     2701 ggttccggat attcctgata ggctacatga gggttctgca tgaccggcga cagccatagt
     2761 gtattgaagc ccatatt
//
LOCUS       DAOMAX010000334         2770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7886_length_2770_cov_5.146593, whole genome shotgun sequence.
ACCESSION   DAOMAX010000334 DAOMAX010000000
VERSION     DAOMAX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7886_length_2770_cov_5.146593"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PK742_12425"
     CDS             <1..442
                     /locus_tag="PK742_12425"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HQU77442.1"
                     /translation="YDEINGQLYFKETHLQEILKLGRCTVPVEIDTLMMIDSLQMTPH
                     HRSRIVDACRTCRHHRIIITHGTDTMVDTAKVLAEAGMDKTIILTGAMIPYKFGSSDG
                     LFNMGCSLSFVQTLPPGVYVAMNGRYFSWDKVRKNRITGIFEEI"
     gene            complement(439..939)
                     /locus_tag="PK742_12430"
     CDS             complement(439..939)
                     /locus_tag="PK742_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU77443.1"
                     /translation="MHKEIVFIVDDEPMHAQMMEDHLRSKYPHFEIHSYKTGEECVDN
                     MDKNPGIVILDYHLDSIDKTARNGIEILNYITKQYPDTYTIMISGQDNIDVAVECMRS
                     GAYDYVVKNETSFVRTEAAIERLFQHRVVLHSNKQYKLVNRVLTIGMIAIVLFSLFMY
                     FTGRIH"
     gene            complement(965..1324)
                     /locus_tag="PK742_12435"
     CDS             complement(965..1324)
                     /locus_tag="PK742_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQU77444.1"
                     /translation="MNAHEQIVNISFLEQFTNGDRTKMVKYINMFLQSAPGSIHTMKE
                     QLAAGHFLDLRTTAHSLKPQLTYMGINGLKETILRIEEYAGQSIKPEEIARLIGEVEN
                     TCLAAFDELKSLVEKMS"
     gene            complement(1321..2670)
                     /locus_tag="PK742_12440"
     CDS             complement(1321..2670)
                     /locus_tag="PK742_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU77445.1"
                     /translation="MIRLLTIAASIWPMRDPWYMDLLFVALAVAVVLLLIRRRIRASA
                     DTQKWLREKVKEKTHELELEMERAEQSEKAKEQFLANMSHEIRTPLNAILGTTRLLLD
                     KAPREDQLRYLNAIKESSDNLLVIINDILDLSKIEAGKIMFEHISFSIREKAHAVLNA
                     LKLTSPEKGLEFRLEVDEDVPDQLIGDPYRLNQVLMNLAGNAVKFTEKGEVAIRIHKL
                     DQTAEESVLRVEVADTGIGISPDKLQHIFDMFTQETTSTTRKFGGTGLGLSICKRLIE
                     MQGGNIEVNSTPGKGSVFSFTIAYGLGKIQSEPQQSTEKEDIQQVRLHNLHILLAEDN
                     EFNKMVAVDTLEELIPDVTIDVAGTGKEAVEMAVKNTYDVILMDIQMPEMDGYEATRL
                     IRSYDDERINAVPIIAMTASVIQAEVDKCFESGMNAFVGKPFNPDELLEKIAQNSKR"
BASE COUNT          646 a          645 c          674 g          805 t
ORIGIN      
        1 gtatgatgag atcaacggtc agctgtattt taaagagacc catctgcagg agatcctcaa
       61 actgggtcga tgcaccgttc cggtagagat cgacaccctg atgatgatag acagtctgca
      121 aatgaccccg catcaccgaa gcaggatagt agatgcctgt agaacctgca ggcaccaccg
      181 catcattatc acacatggaa ccgataccat ggtagatacg gctaaggtat tggcagaagc
      241 tggaatggat aagaccatca tcctgacagg agctatgata ccttataagt ttggcagttc
      301 tgacgggttg ttcaatatgg gatgttccct gtcattcgta caaacccttc ctccaggggt
      361 ttatgtggcc atgaatggcc ggtatttttc ctgggataag gtgcggaaga accgcattac
      421 cggaattttt gaagagatct agtggatcct tccggtaaag tacatgaata aggagaacag
      481 tacaatggcg atcataccga tggtaagcac ccggttcacc agcttatact gtttattgct
      541 gtgcagtacc acccgatgct gaaacaggcg ttcgatagca gcttcagtac gcacaaaact
      601 ggtctcgttc ttcaccacat agtcataggc tcctgaacgc atgcattcaa cagctacgtc
      661 aatattatcc tgaccggata tcatgatggt gtaggtatca ggatactgct tggtgatgta
      721 attcaggatc tcaataccgt tacgggcggt cttgtcaatg gaatccaggt ggtagtccag
      781 tatcacaatg ccgggattct tgtccatatt gtccacacac tcttctccgg ttttatagga
      841 atggatctcg aaatgcgggt atttgctgcg caggtggtct tccatcatct gggcgtgcat
      901 gggttcatcg tcgacaatga atacgatctc tttgtgcata ggttgtctgg tttggcaggt
      961 aaaattagga cattttctcc acgagacttt ttaactcgtc aaaagcggca agacaggtat
     1021 tttctacctc gccgatcaaa cgggcgatct cttccggttt tatggactga cctgcatatt
     1081 cttcaatgcg cagtatggtt tccttcagcc cgttgatacc catataggtc agctgcggtt
     1141 tcaggctgtg ggcagtggtt ctgagatcga gaaaatggcc cgctgccagt tgttctttca
     1201 tggtgtgaat ggaaccgggt gcagactgca ggaacatatt gatgtatttg accattttgg
     1261 tcctgtcgcc attggtgaac tgttccagaa aactgatatt cacgatctgc tcatgtgcat
     1321 tcatcgctta ctgttttgtg ctattttttc aagtaattcg tcgggattga aaggttttcc
     1381 cacaaaggcg ttcataccgc tttcgaagca tttgtccacc tcggcctgaa tgacgctggc
     1441 tgtcatggct atgatcggca cggcattgat cctttcgtca tcataacttc tgatgagacg
     1501 ggtggcttca tagccgtcca tctccggcat ctgaatatcc atcaggatca catcataggt
     1561 gtttttaact gccatttcaa ctgcttcttt tccggtgcca gccacatcta tggtcacatc
     1621 cggaatgagt tcttccaggg tatctaccgc taccatttta ttgaactcat tgtcttcggc
     1681 caggagaatg tgaaggttgt gcaacctgac ttgttgtata tcttcttttt ctgttgattg
     1741 ctgaggttct gactggatct tgccaaggcc ataagcgatg gtaaaggaaa agaccgaacc
     1801 tttacccgga gtactgttta cctcgatatt gccgccttgc atttctatca ggcgtttgca
     1861 gatggacaat cccagaccgg tgccaccaaa ttttctggtg gtactggtgg tctcctgggt
     1921 gaacatgtca aagatgtgct ggagcttgtc tggacttatt ccgattccgg tgtctgctac
     1981 ttctacccgg agaacagatt cttcggcggt ctgatcaagc ttgtgaatgc gtatggctac
     2041 ttcacctttt tcagtgaact tgacagcatt ccccgccagg ttcatcagta cctggttcag
     2101 gcggtaggga tcgccgatga gctggtcagg cacatcttca tccacttcca gccgaaactc
     2161 aagtcctttt tccggagagg tcagcttcag tgcattcagt accgcatggg ctttttcacg
     2221 aatactgaaa ctgatgtgct caaacatgat cttaccggct tcgatcttgg agaggtccag
     2281 gatatcgtta atgatgacca gcaggttgtc tgaagattct ttgatggcat tcaggtatct
     2341 gagctgatct tctctgggtg ctttgtccag cagcaaacgg gtagtgccaa ggatggcatt
     2401 cagaggggtt ctgatctcat ggctcatatt ggccagaaac tgctcttttg ctttttctga
     2461 ctgctctgca cgctccattt ccagctccag ttcatgggtc ttttctttga ccttttcacg
     2521 cagccacttc tgggtatctg ccgatgctct gatgcgcctc ctgatgagca gcagaaccac
     2581 tgctacagct aaggctacga acaacagatc catataccag ggatctctca ttggccagat
     2641 tgatgcagca atggtgagga gacggatcat ggcatgaagg aaatcaaatg tacctaattc
     2701 aacttaaatg gaaggtcagg tctgtgtgtt gttcagggat catggagcgt tccagatacc
     2761 tgttgcctgt
//
LOCUS       DAOMAX010000335         2753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_7977_length_2753_cov_3.970348, whole genome shotgun sequence.
ACCESSION   DAOMAX010000335 DAOMAX010000000
VERSION     DAOMAX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7977_length_2753_cov_3.970348"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..2442)
                     /locus_tag="PK742_12445"
     CDS             complement(127..2442)
                     /locus_tag="PK742_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQU77446.1"
                     /translation="MLSRKTLFPVLVICSLILFATFAFTSANQRSSLMHRLMGKDGKT
                     MMEEIPWDSSWTGNEGMKALLSNIYEKTNVPSCRFCSCAIADELLSRPIPEKWDDRYE
                     YHSRLVSELINCGRMETAMQWIDRFDAEMTSRKVDNKFRRKWLASKGIAFLRYAEVTN
                     CIQNHNEESCIFPLSEKAQHRNETGARAAIKLYSQILEMYPDDLASRWLLNIAYMQIG
                     GYPQDVPVAWLIDPAAISQGEEVPAFRNIASTLGVDFPDMCGSVIMDDFNNDGLIDIF
                     QSGWGLQEQVHFLINKGDGSFEDQTAAAGLEGYPGGLMMQQTDYNNDGLLDVFILRGA
                     WYGAQGVIPNSLLHNNGDGTFTDVTRETGLYSCHPTQTATWADFNNDGWIDVFIGNEA
                     SRKKADQRNDSELYLNDHGHFRNVAAASGANVNGFIKGVSSGDYDNDGDPDIYVSING
                     SENILLRNDSRRGSMELHFTDVTREAGVADPVKSFPCMFFDANNDGWLDILTFSYSSA
                     VSDNDIPAEYLGLPRQGNDPALYINNGNGTFSNETIAWGLDKTFLVMGCNYGDFDMDG
                     WQDFYTGTGKPSLRSIIPNRMFRNDNGTGYVEVTSAARVGHLQKGHGISFADLNNDGY
                     PEIYAQMGGAYESDGFQDALYENPASWGTGWITLDLTGNETNKAAIGARVEVVVKENG
                     KERHIYDWVVPGASFGANSLRLEIGLRQAEEIVRVEVYWPGSDEVQSWTGLHLRHHYR
                     LQEGKPAAEELVLPEFDFPATSHQSNHHHMM"
     gene            complement(2564..>2753)
                     /locus_tag="PK742_12450"
     CDS             complement(2564..>2753)
                     /locus_tag="PK742_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77447.1"
                     /translation="AAKIDSVTVDWPGGSSETFADIPLKSCVQLKQGTGMIGTKELHP
                     FRFTHAPDQMDHSMHMEM"
BASE COUNT          658 a          715 c          631 g          749 t
ORIGIN      
        1 caggtcgttg ctgcaggcat accacattcc cgcggccagc acggcgacaa atcctgttaa
       61 ccgaagatcc atgcccccaa atatagggag gctgctatag cctttggtcg gttccgggtc
      121 aaatgatcac atcatatggt gatgatttga ctgatggctg gtagcgggaa aatcaaattc
      181 aggcagcacc aattcttcgg ctgctggctt tccttcctgc aggcgatagt gatgacgaag
      241 atgcaggcct gtccaggatt gcacctcatc tgagcccggc cagtacactt ccacccggac
      301 gatctcttca gcttgtctga gacctatttc cagacgcagg ctgttggcac cgaaactggc
      361 gccgggcact acccagtcat agatatgtct ttcctttccg ttttccttca ccaccacttc
      421 caccctggca ccgatggctg ccttgttggt ttcgttgccg gtcagatcaa gggtgatcca
      481 gcctgttccc cacgaagcgg gattttcata cagggcatcc tgaaatccat cagactcata
      541 ggctccgccc atttgtgcgt agatctccgg atacccgtcg ttgttgaggt cggcgaaaga
      601 aatgccatgt cctttctgca ggtgccccac cctggcagcg ctggtcactt ccacatatcc
      661 tgtaccgtta tcattgcgaa acatcctgtt ggggatgatg cttcgcaggg aaggctttcc
      721 ggttccggta tagaaatcct gccatccgtc catgtcaaaa tcgccgtagt tacatcccat
      781 taccaggaag gttttatcaa gcccccatgc aatggtttca ttgctgaaag ttccattgcc
      841 attattgata tacaaggcag gatcattccc ctgacgtgga aggcccagat actcagcagg
      901 tatatcattg tctgacactg cagaactata gctgaaggtc agtatatcca gccatccgtc
      961 attattggca tcaaagaaca tgcaaggaaa actcttgacc ggatcggcta cgcctgcttc
     1021 ccgggtcaca tcagtaaaat gaagttccat actgccacgc cgggaatcat tgcgcagaag
     1081 tatattttct gatccgttaa tggatacata gatatccgga tctccgtcgt tgtcataatc
     1141 gccgctactc actcctttaa tgaatccatt cacattggct cctgatgctg cagctacatt
     1201 tcgaaagtga ccatggtcgt tcaggtataa ttcagagtcg tttcgctgat ctgctttttt
     1261 gcgggaagct tcattaccga tgaacacatc tatccatcca tcgttgttga aatcagccca
     1321 tgtagcggtc tgtgtaggat ggcagctgta caatcctgtc tctctggtca catcagtgaa
     1381 agtaccatcg ccgttgttgt gcaacaggga gttggggatc accccctggg caccgtacca
     1441 ggccccgcga aggatgaata catccagcag gccatcgtta ttgtaatctg tttgctgcat
     1501 catcaatccg cccggatagc cttccagacc agccgctgct gtttggtctt caaagcttcc
     1561 gtcacccttg ttgatgagaa agtgaacctg ctcctgtaat ccccatcccg actgaaagat
     1621 gtcgatcagt ccgtcgttgt tgaaatcatc catgatgaca gatccgcaca tgtcgggaaa
     1681 atctacaccg agggtggagg cgatgttcct gaaagccggt acttcttcac cttggctgat
     1741 ggctgccgga tcaataagcc aggcaacagg tacatcctgc ggataaccac ctatctgcat
     1801 gtaagcaata ttgagcagcc agcgggaagc caggtcatca ggatacatct ccaggatctg
     1861 tgagtacaat ttaatggcag ctcttgcacc tgtctcgttc ctgtgttgtg ccttttcaga
     1921 cagcgggaat atgcaggatt cttcgttgtg gttctgaata caatttgtca cttcagcata
     1981 cctcagaaat gcaattcctt tagaagccag ccattttctt ctgaatttat tgtccacctt
     2041 tctgctggtc atctcggcat caaaacggtc tatccattgc atggcagttt ccatacgtcc
     2101 gcagttgatc agttctgaaa ccagcctgga atggtattca taacgatcat cccatttttc
     2161 cggaataggt ctggatagga gttcatctgc aatggcgcag ctgcaaaacc tgcaggaagg
     2221 cacattggtc ttttcatata tattggaaag caatgctttc atgccctcat taccggtcca
     2281 ggatgaatcc caggggatct cttccatcat ggttttgcca tcttttccca tcagacgatg
     2341 catcaggctg cttcgctggt tggcagaagt gaaagcaaat gtggcaaaca ggatcagtga
     2401 acagataacc aggacaggaa acagtgtttt gcggctaagc atacgagcag gattttgtgc
     2461 aaaattcctg cagcagctac ccctttccta tttacttatg taaatctatc ctgttactta
     2521 tttaacagga tgtaaagtac tttaaaatga tcccaacggc tgtttacatt tccatatgca
     2581 tggaatgatc catctgatcc ggagcatgtg tgaagcggaa gggatgcagt tctttagttc
     2641 cgatcatgcc cgtgccttgt ttgagctgca cacagctctt caaaggaata tctgcaaagg
     2701 tttctgaact gcctccgggc cagtcaactg taacgctgtc gatctttgct gca
//
LOCUS       DAOMAX010000336         2737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8056_length_2737_cov_4.325130, whole genome shotgun sequence.
ACCESSION   DAOMAX010000336 DAOMAX010000000
VERSION     DAOMAX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8056_length_2737_cov_4.325130"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..2123)
                     /locus_tag="PK742_12455"
     CDS             complement(360..2123)
                     /locus_tag="PK742_12455"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQU77448.1"
                     /translation="MSSVANSPQITYVVSMPQPENHYFHIEITVKGLKGKEHDWFMPV
                     WTPGSYLVREFPKNVTEVRAWDLDYVTIPVHKKDKNTWTIAHNKKDFVISYSVYAFEE
                     SVRTSFLDADRALFMPSSTCLYPTELDVPVTLKFIPPSVSSEFNLSQVAVHWKDISTP
                     MEQGSDPWTRICHSRDELFDSPVEMGNHAVFPFKAGGNDNYLVMTGEGNYHADTLVDY
                     LQKIIDEETAVFGSNPAQPYYFLVNNTQQRGGGLEHKFSSAIQYSRDGYGGDSYKSFL
                     ALMAHEYFHIWNVKRLRPEALGPFDYSQENYTTSLWIVEGFTSYYDDLILQRTGIYSK
                     DEYLSIAGRNIHNVVNNTGDVYQSVADASFDAWIKAYRPNENSGNVEVSYYSKGGVLA
                     MFLDMYIIGQSNGKYCLDDVMRAAYDRFYLQRDKGYTEAEFKAVLEEFAGSSLDNFYR
                     DHVHGTLPLNVNSFVSNVGLEWIPGEADKTAWFGMRVTGNNTIYSVQPDGPAAMYGLN
                     AEDIIVAVNNVALTEGFETIRQTLQPGEPVQITVVRRGLPKTFTVVPTINPRPRFTLQ
                     PVASPTEQQKMNFRKWMQEEH"
     gene            complement(2185..>2737)
                     /locus_tag="PK742_12460"
     CDS             complement(2185..>2737)
                     /locus_tag="PK742_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQU77449.1"
                     /translation="VVPELKKQQYNTEEIIEELKRSMEDITQISEEDKDSIITNITRD
                     LDKEDPVYREVEDSIYMVPIREGQVLTLNNVFFDANKSKIKEESFSELNEIAVFLKEN
                     PNIWVEIGGHTNGLPEDDFCNKLSNDRAESVRQYLIGQGADADRITWKGYGKTQPIAD
                     NSTLAGRTKNQRVELKIIKVEGR"
BASE COUNT          649 a          678 c          637 g          773 t
ORIGIN      
        1 atatctgggt ttcagaattt tcagcatctg aatcagatac ctttaaaatg aagtgaccct
       61 tgtattgtcg ctttctgtag gcttctgcca gatttgcaca gacacttctg ctggatcttc
      121 ttatctggtc tgtaagtgaa tacttttctt caacaggaaa tgctttactc acctcgaaaa
      181 ttttcattga caggtcaaaa gctttttcaa atactagcaa gtcagtcagt ccgcccatag
      241 catgtcagat tattggggat aagcaacctg gcaatactaa taaaaattaa tgtattacaa
      301 ataagactaa acgtgttgtg ataatttgcg aactgcccac cgccaattgc aaactgatct
      361 caatgctctt cctgcatcca cttcctgaag ttcatctttt gttgttcagt aggagatgct
      421 acaggttgca aagtaaagcg tggtctgggg ttgatggttg gcaccaccgt aaaggtcttg
      481 ggtagtccgc ggcgcaccac agtgatctga acgggttctc ccggttgtaa ggtctgccgg
      541 atggtttcaa atccttcggt cagcgccaca ttgttcacgg caacgatgat gtcttcggcg
      601 ttcagaccgt acatagctgc aggaccatct ggctgtaccg aatagatcgt attgttgcct
      661 gtgacccgca tgccgaacca ggcagtctta tctgcttcgc cggggatcca ttccagaccc
      721 acattcgata cgaaagagtt cacattcagc ggtagtgtgc cgtgtacatg atctctgtaa
      781 aaattatcca gtgatgagcc tgcaaattct tccagcaccg ctttgaattc ggcttccgtg
      841 tatcctttgt cccgctgcag ataaaagcga tcgtaagctg cacgcatcac atcatccagg
      901 caatatttcc cgtttgactg cccgatgata tacatatcaa ggaacatagc cagcacgcca
      961 cctttggaat aataactgac ctccacattc ccgctgttct cattcggccg gtaggctttg
     1021 atccatgcgt caaaggaagc atctgccaca ctttgataca cgtcaccggt gttgttcacc
     1081 acattatgga tgttccttcc tgctatactc aggtattcgt ctttgctgta aatgccggtt
     1141 ctttgcagga tcaggtcatc ataataggaa gtgaatcctt ctactatcca gagtgaagta
     1201 gtgtaattct cctgagaata atcgaagggt ccgagggctt ccggccgcag acgcttgaca
     1261 ttccagatat ggaagtattc atgcgccatc agggccagaa aacttttgta actgtcgccg
     1321 ccatatccgt cgcgggaata ctgaatggcg gaagagaatt tgtgctccag tccgccgcct
     1381 cgctgctggg tattgttgac cagaaaataa taaggctggg caggattgct gccaaacacg
     1441 gccgtctctt catctatgat cttctgcaga tagtccacca atgtatcggc gtggtagttg
     1501 ccttcaccgg tcatgacgag gtaattgtcg ttgccacctg ccttgaatgg aaacaccgcg
     1561 tggttgccca tctctaccgg actgtcgaac aattcatcgc ggctgtggca aattctggtc
     1621 cacggatctg aaccctgttc catcggcgta ctgatatcct tccagtgaac agccacctgt
     1681 gacagattga actcggaaga cacggagggc ggaataaatt tcagggtgac aggcacatca
     1741 agctctgtcg gatacaaaca agtggaactg ggcatgaaca aggcacggtc tgcatccaga
     1801 aaagaagttc tgaccgactc ttcaaaagca tagacagagt agctgatcac aaagtccttt
     1861 ttattgtggg ctatggtcca ggtgttcttg tcttttttgt gcaccggaat ggtcacatag
     1921 tccaggtccc aggccctgac ctcggtgacg ttcttcggaa attcacgcac cagataagag
     1981 ccgggtgtcc atacaggcat gaaccagtcg tgttcctttc ccttgagtcc tttgaccgtg
     2041 atctctatat ggaaataatg attttcaggc tgaggcatgc tgaccacata ggtgatctgc
     2101 ggagagtttg caacagaaga cataagcagt gctaatgata acaacatatt catgggatca
     2161 gccgcaaata taattgctgg gtgcttagcg accctctacc ttgatgatct tgagttccac
     2221 ccgctggttc ttcgtcctgc ccgcaagggt gctgttgtcg gcaatgggct gggtcttgcc
     2281 atatcctttc caggtgatgc gatcggcatc tgcaccctga ccgatcaggt actgacgcac
     2341 actttctgcc cggtcattgg agagtttgtt gcagaaatca tcctccggaa ggccgttcgt
     2401 atgtccgcct atctccaccc agatgttcgg gttctctttc aggaagacag ctatttcatt
     2461 cagttcgcta aaagactctt ccttgatctt agacttgttg gcatcgaaga atacattgtt
     2521 aagagttagc acctgtcctt cgcggatggg taccatgtat atgctgtctt ccacttcgcg
     2581 gtacaccggg tcttccttat ccagatcgcg ggtgatattg gtgatgatac tgtccttgtc
     2641 ttcctctgat atctgggtga tatcttccat gctgcgcttc agttcttcga tgatctcttc
     2701 cgtgttatat tgctgcttct tcagttcagg aaccacc
//
LOCUS       DAOMAX010000337         2719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8154_length_2719_cov_3.284535, whole genome shotgun sequence.
ACCESSION   DAOMAX010000337 DAOMAX010000000
VERSION     DAOMAX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_2719_cov_3.284535"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PK742_12465"
     CDS             <1..596
                     /locus_tag="PK742_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU77450.1"
                     /translation="VYLIGKTGTGKSSLLKTIYGDLPLQGGSAWVAGFPLHEITWKKT
                     PYLRRKLGIVFQDFQLLMDRSVEANLEFVLRATGTSDQALIKIKIDEALRRVGLATKG
                     FKMPYELSGGEQQRVVIARAIINDPEIIIADEPTGNLDPETSNDIMLLLRHLNEEFKT
                     AVLMATHNYNILEKWPSKTLRCINGKLQVELPESISS"
     gene            complement(550..1695)
                     /locus_tag="PK742_12470"
     CDS             complement(550..1695)
                     /locus_tag="PK742_12470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU77451.1"
                     /translation="MSVDRHIHVVSFNVPYPADYGGVIDVYCRLQALHREGVSITLHC
                     FDYGRGEQPELNKLCQEVYYYKRRSLWRGLTSSLPLFVAARRSSLLTARLLLDDDPIL
                     LEGLHTSALMLDHRIDRKRCYIRLHNDEAVYYRSLAGRERSWWKRLYFELEARRLEQF
                     EHILEQAAGVFTISRPEQEKFAGKYKNILYTGPFHAWQQTEIKTGRGSYILYYGNLSV
                     NENEEAVRYLLDELSSSLPAELVIAGKEPSPQLQAAIAGKKQVRLISDPDQNTMLDLI
                     RQAQVLVLPTFQPTGIKLKLIDALFNGRHIIVNSPMIAGTGLEDLVTIADDPVALRQM
                     VQQAWETEFTDEMVSRRRAHPALPDNRQAVTVIMKKCFPVIPPAASR"
     gene            complement(1685..>2719)
                     /locus_tag="PK742_12475"
     CDS             complement(1685..>2719)
                     /locus_tag="PK742_12475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQU77452.1"
                     /translation="RICTTLQDAGYQVTLVGFTKKRSVPLVQNMYATVRLNPFFKKGK
                     LFYLEYNFRLLLWLIKHPSDIYGAVDLDTLLPHVLISSWRKKICVHDAHEYFTELPEI
                     AHRPVIKSLWEWMARMLLPKVPYNYTVCTAIAHELQQRYGTTYAVIRNVPEIPPEMPV
                     RDPVSPPVFLYQGALNRGRGIEQMMEAIGHVDASLWLAGEGDLSDELRQKAAAFPWAD
                     RIRFLGYIEPRVLPSVTAQAFAGMNLVSNDGKSYYYSLSNKFFDYIRTGIPQITMNYP
                     EYRSLNEQYGVALTINSLEVADIRRAMESLLTNHDLYSQLAQNTVSAFKELNWEVEKM
                     KLLQFYEHVS"
BASE COUNT          722 a          670 c          629 g          698 t
ORIGIN      
        1 tcgtttatct gataggcaag accggcacag gaaagagcag tctgctcaaa accatttacg
       61 gtgatctgcc cctgcagggt ggttcggcct gggtggcagg gtttcccttg cacgagatca
      121 cgtggaagaa gactccttat ctgcgccgga aactgggtat cgtatttcag gattttcagc
      181 tgctgatgga cagaagtgtc gaggccaatc tggaattcgt tctgcgggcc accggtacct
      241 cagaccaggc attgatcaag atcaaaatag atgaagctct acgtcgtgtt ggcctggcca
      301 ctaaaggctt taagatgccc tatgaattat cgggtggtga gcaacaaaga gtggtcatag
      361 ccagggccat catcaatgac ccggagatca tcattgctga tgagccaacc ggaaatcttg
      421 accccgaaac atccaatgat atcatgttgc tgttgcgcca tctcaatgag gaatttaaaa
      481 cggcggtgct gatggccaca cacaattaca atatcctgga aaaatggccc tccaaaaccc
      541 tgcgctgtat caacgggaag ctgcaggtgg aattaccgga aagcatttct tcataataac
      601 agttactgcc tggcggttat caggcagggc gggatgcgct ctcctcctgc tgaccatctc
      661 atcagtgaac tctgtttccc atgcttgctg caccatttga cgtaaagcaa caggatcgtc
      721 agctatggtc acaagatctt cgagcccggt gcctgctatc atagggctgt tcacaatgat
      781 atgtcggccg ttgaaaagag cgtcgatcag ttttagtttg atacctgttg gctgaaaggt
      841 gggaaggacc agaacctggg cctgacgaat aagatccagc atggtattct gatcgggatc
      901 tgagatgagt ctgacctgtt tctttccggc gatggccgct tgtagctgag gtgatggctc
      961 tttaccagcg atgaccagtt ctgcaggcaa tgacgaagac agttcatcca gcagatatct
     1021 tacagcctct tcgttctcat tcacacttag gttaccataa tacagtatat agctgcctct
     1081 tcctgttttg atctctgttt gctgccaggc atggaacgga cccgtataga gaatgttctt
     1141 atattttccg gcaaatttct cctgttccgg tctggagatc gtaaaaacac ctgcagcctg
     1201 ttcaagtata tgttcaaatt gttcaagccg tcgagcctcc agttcaaaat acagccgttt
     1261 ccaccaggat ctttcccggc cggccagcga cctgtaatag accgcttcat cattatgcag
     1321 cctgatgtag caacgtttcc ggtcaatcct gtgatcgagc atcagagctg aagtatgtaa
     1381 tccttccagc aagatggggt catcatccag caacaaacga gctgtcagca gagatgatct
     1441 tcgtgctgct acaaacaaag gaagcgagct tgttaatcct cgccacaagg atcggcgttt
     1501 gtagtaataa acttcctggc acagcttgtt caattcaggt tgttcacccc ttccataatc
     1561 aaagcaatgc agtgttatgg aaactccttc ccgatgcaaa gcctgcaggc gacagtacac
     1621 atcaatgacc ccaccgtaat cggccggata aggcacattg aacgatacca catgtatatg
     1681 tctatcaact gacatgctca taaaactgca gcaatttcat cttttccact tcccagttca
     1741 gctccttgaa ggcagagacg gtattctgag ccagttggct gtacaggtca tggtttgtta
     1801 acaaagattc catcgctctt ctgatatctg ctacttcgag gctgttaata gtaagcgcca
     1861 caccatactg ttcattcagg gaccggtatt cgggataatt catcgtgatc tgaggaatac
     1921 cagttcttat ataatcaaaa aacttattag acaaactata gtagtaactc ttgccatcat
     1981 tgcttaccag attcatgccg gcaaatgcct gagctgtgac agacggaagg acacggggtt
     2041 caatataacc aagaaaacga atgcggtcag cccagggaaa agcagctgcc ttctgtctga
     2101 gttcatcaga aagatcgccc tctcctgcca gccaaagcga tgcatccaca tgaccgatgg
     2161 cctccatcat ctgttctata ccccttcctc tgttgagggc accctgatac aaaaaaaccg
     2221 gaggcgaaac agggtcccga accggcattt caggcggaat ttccggcaca tttctgatga
     2281 ccgcataggt ggtgccatat cgctgctgca attcatgtgc aatggcagta caaacggtgt
     2341 aattatatgg cacttttggc agaagcatgc gggccatcca ctcccataag gactttatta
     2401 ccggcctgtg ggctatttca ggcagttcag taaaatactc atgtgcatcg tgtacacaga
     2461 tctttttgcg ccaactgctg atcagcacgt gcggaagcaa tgtgtccagg tcaacagctc
     2521 catagatatc agaaggatgt tttataagcc acaacagcaa gcgaaagtta tattccaggt
     2581 agaacaattt gcccttctta aaaaacgggt tcaatctgac ggttgcatac atattctgaa
     2641 ccagcggaac cgatcttttt ttggtaaaac caacaagtgt cacctggtat ccggcatcct
     2701 gcaaggtagt acatatacg
//
LOCUS       DAOMAX010000338         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8334_length_2689_cov_4.292331, whole genome shotgun sequence.
ACCESSION   DAOMAX010000338 DAOMAX010000000
VERSION     DAOMAX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8334_length_2689_cov_4.292331"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK742_12480"
     CDS             <1..485
                     /locus_tag="PK742_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQU77453.1"
                     /translation="TITNHGSDRKYYYDSIGVNSRLDSFQAAVLVAKLAKLDQYNAAR
                     KQAADYYDQQLTGIHQVTVPARTSHSDHVFHQYTIRVTDGRRDALQQYLGEQGIPSMI
                     YYPVPLHLSEAYKSFGYNAGDFPVSEQLSSEVLSLPMHTELTAEQLDHIIFHIRNFFA
                     "
     gene            499..1551
                     /gene="rfbB"
                     /locus_tag="PK742_12485"
     CDS             499..1551
                     /gene="rfbB"
                     /locus_tag="PK742_12485"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQU77454.1"
                     /translation="MKKVVITGGAGFIGSHLLRRMVNRYPDVTFVNLDKLTYAGNLEN
                     LLDIEHKPNYRFIKGDIVDGDFLTALFDAEQFDTVIHLAAESHVDRSIANPIEFVMTN
                     VVGTVNLLNAARHCWNGNWEGKMFYHVSTDEVYGSLGEEGFFEESTPYDPHSPYSASK
                     ASSDHFVRAYHDTYGMPVLLSNCSNNYGPYQFPEKLIPLAIHNIKNSKPIPVYGKGEN
                     VRDWLWVEDHAAAIDLILQEGTTGETYNIGGFNEWKNIDLIKLLCTIMDKKLGRPEGT
                     SAGLITFVRDRAGHDLRYAIDASHLNTSLGWKPSLQFEEGLEKTVDWYLANEEWLDHV
                     TSGTYKQYYQQQYADR"
     gene            1551..2120
                     /gene="rfbC"
                     /locus_tag="PK742_12490"
     CDS             1551..2120
                     /gene="rfbC"
                     /locus_tag="PK742_12490"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQU77455.1"
                     /translation="MPFTETPISGLLVFEPRVFSDDRGYFFESFNEQTFAEAGITRPF
                     VQDNQSRSVYGVVRGLHYQLAPMAQAKLVRVVEGEVLDVAVDIRQGSPTFGQHFSILL
                     SKENKKQLYLPRGFAHGFSVLSEYAEFVYKCDNFYSGGHDAGIRFDDPALGIDWKLPA
                     DDVLLSGKDAALPLFADAKNNFTEEGTAL"
     gene            2117..>2689
                     /gene="rfbD"
                     /locus_tag="PK742_12495"
     CDS             2117..>2689
                     /gene="rfbD"
                     /locus_tag="PK742_12495"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HQU77456.1"
                     /translation="MKILVTGSNGQLGSELRRLFAERTADDCLFIDIGELDITDPVAV
                     RSFFHEHRPEVCINCAAYTAVDKAESEPDLARKVNVTGVEVLAKNCEDSGASLVHIST
                     DFVFNGEADKPYSTDHPVSPVSVYGLTKAEGENRALSTCSRTWLVRTSWVYSAFGGNF
                     VKTMIRLGKERSEINVVNDQVGSPTWAKDLA"
BASE COUNT          757 a          599 c          656 g          677 t
ORIGIN      
        1 gcaccattac caatcatgga tctgacagga aatactatta tgattctatt ggtgtgaatt
       61 cacgtctgga tagcttccag gcagctgtgc ttgtagccaa gcttgcgaaa ctggatcagt
      121 ataacgcagc cagaaaacag gccgccgatt actacgacca gcaattgact ggcattcatc
      181 aagtgaccgt tcctgcacgc acctcccatt cagaccatgt atttcatcag tacaccatac
      241 gggtaacaga tggcagaaga gacgccctgc aacaatacct gggtgaacaa ggcatcccat
      301 ccatgatcta ttatccggtt ccgctccacc tgagtgaagc ctataaaagt tttggataca
      361 atgcaggcga ttttcccgtt agtgagcaac tttcatcaga agtgttgtca ctacccatgc
      421 acaccgaact gaccgccgaa caactggatc acatcatatt tcatatccgt aatttttttg
      481 cataagaagt cgcagtaaat gaaaaaagta gtcataacag gaggtgccgg ttttataggc
      541 tctcacctgc tcagaagaat ggtgaatcgc tatcctgatg taaccttcgt caacctggat
      601 aaactcacct atgcaggaaa cctggagaac ctgctggata ttgaacataa accaaactac
      661 aggttcatta agggtgatat agtagatgga gattttctta ctgccctgtt cgatgctgag
      721 caatttgata ccgtcatcca cctggcagcc gaatcacatg tggacaggtc tatcgccaac
      781 ccgatagaat ttgtcatgac caatgtggta ggtaccgtga acctgctcaa tgcagccaga
      841 cattgctgga atggaaactg ggaaggaaag atgttctacc atgtgtcaac agatgaagta
      901 tatggcagcc tgggagaaga agggtttttt gaagaatcta ctccttatga cccgcatagc
      961 ccttattctg catcaaaagc cagttcagac cattttgtac gtgcctatca cgacacctat
     1021 ggcatgccgg tgttgctgtc aaactgttcc aataattacg gcccttacca gtttcctgaa
     1081 aaactcattc ctcttgcgat ccataacatt aagaacagca agccaattcc tgtttatgga
     1141 aagggagaga atgttcgcga ttggttatgg gtggaagacc atgcagctgc cattgatctg
     1201 atattacagg aaggcactac aggtgaaacc tataatatcg ggggcttcaa tgaatggaag
     1261 aatatagacc tgatcaaatt gctgtgtacc atcatggata aaaaactggg tcgcccggaa
     1321 ggaacttctg caggtctcat cacttttgtc agagaccgtg ccggacatga ccttcgttat
     1381 gccatagatg cctcgcacct gaatacatcg ctgggctgga aaccttcatt acagtttgaa
     1441 gaaggactgg agaaaacagt tgactggtac ctggccaatg aagaatggct ggatcatgtg
     1501 accagcggta cttataagca atactatcaa cagcaatacg ccgacagata atgccattca
     1561 cagaaacacc gatcagcgga ttgcttgtat ttgaacccag ggttttcagc gatgatcgcg
     1621 gatacttctt tgaaagtttc aatgaacaga cctttgcaga agcaggtatc accaggcctt
     1681 ttgtacagga taaccagagt cgttccgtat atggagtagt tcgtgggctg cattatcagc
     1741 tggcgcctat ggcacaggct aaactggtca gggtagtaga gggggaagtg ctcgatgtag
     1801 ccgttgatat cagacagggt tcccccactt tcggacaaca tttcagtatt ttactaagta
     1861 aggaaaataa gaagcaacta tatctgccaa gaggatttgc tcatgggttt tcagtactaa
     1921 gtgaatacgc agaattcgta tataaatgtg ataactttta ttcaggtgga cacgatgcag
     1981 ggatccggtt tgatgaccct gcacttggaa tagactggaa attaccggcc gatgatgtgt
     2041 tgctgtcagg caaggatgca gcacttccgt tatttgctga tgcaaagaac aattttacgg
     2101 aggagggaac agccttatga agatcctggt aacaggcagc aacgggcagc tgggctctga
     2161 gttgcgacgt ttgtttgcag aacgaactgc ggatgattgc ctgttcattg atatcggtga
     2221 actggacatc actgatccgg ttgcggtgcg cagtttcttt catgaacacc ggcctgaagt
     2281 ttgtatcaac tgcgcagcct ataccgcagt tgacaaagca gaatctgaac cggatcttgc
     2341 tcgtaaggtc aatgtaaccg gggtagaggt gctcgcgaag aactgtgaag attccggggc
     2401 atcactggtg catatcagta cagattttgt gttcaacgga gaagctgaca agccgtattc
     2461 tactgatcat ccggtaagtc ccgtcagcgt gtacggactt accaaagccg aaggtgagaa
     2521 tcgtgctctg agcacctgca gcagaacctg gctcgtcaga acatcctggg tgtactctgc
     2581 ctttggtggt aacttcgtga agaccatgat acgcctgggg aaggaaagat cagagatcaa
     2641 tgtggtcaat gatcaggtag gaagccccac ctgggcaaag gatctggcc
//
LOCUS       DAOMAX010000339         2688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8340_length_2688_cov_3.729206, whole genome shotgun sequence.
ACCESSION   DAOMAX010000339 DAOMAX010000000
VERSION     DAOMAX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8340_length_2688_cov_3.729206"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..305)
                     /locus_tag="PK742_12500"
     CDS             complement(75..305)
                     /locus_tag="PK742_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HQU77457.1"
                     /translation="MKEKDRWTAIALAFLLGGLGVHRFYLGQTGYGLLYLLFCWTFVP
                     TAISVIDAIIWAVQDDNTFNRKYNGFVSGRGD"
     gene            complement(302..1510)
                     /locus_tag="PK742_12505"
     CDS             complement(302..1510)
                     /locus_tag="PK742_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77458.1"
                     /translation="MKRLLPSTIILSLLLLLGSCTVQQLSYTQKTYQNTQKSDETVQV
                     QYTLPVVKPAAKTTQEQTKGGVTISVEVLSFSAHLMEEEEENVAYKIPAQDDYDVFEI
                     RKTPYYRVSPENIRFKIRIRNREDVPLKLSEVGFALIIDGTQWSFPSTHLEEWNKGLI
                     LSGFEKEYFVDGPQLDGLVNAQVVYLFLNGVPVSYDKAGNVTEKKNFEWFFECSSTEV
                     TKEEEVHYEYVSRPIHKERCHKCTGTGKDPQLYKCDKCAGNGAYISKIDGKTYKCSKC
                     DGTGKIQVTCDHCKGTGVLEYPKSTLPPVDQSITWNGWKVTVSTIPKGASIKIVDPET
                     GVYTAAPYNTPVITPWYYTGTDKRPIIIEYNGQTAKILPYHEGAPSARVVVDFSSGTP
                     VVTRGELVAE"
     gene            complement(1544..1885)
                     /locus_tag="PK742_12510"
     CDS             complement(1544..1885)
                     /locus_tag="PK742_12510"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HQU77459.1"
                     /translation="MKITAYHLGCHGIPERCLVINGRRMHICARCAGSNIGHIIALVM
                     VIARIIPAWWVSLLMLGVMMTDWSLQTFANIPSNNTRRLITGILGGIGVGLLIWTGLM
                     LLISYLTRLFY"
     gene            complement(1893..2096)
                     /locus_tag="PK742_12515"
     CDS             complement(1893..2096)
                     /locus_tag="PK742_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77460.1"
                     /translation="MTTTTHTEDRVVCPKCGSTQIHIDKKGYSAAKGCCGFIACGPLG
                     FLFGQSGANKIEKTCLNCNNKWT"
     gene            2332..>2688
                     /locus_tag="PK742_12520"
     CDS             2332..>2688
                     /locus_tag="PK742_12520"
                     /inference="COORDINATES: protein motif:HMM:NF037066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77461.1"
                     /translation="MNYELLFKNYQQKLKSAFEKDAAPQTEPSTGKLLVKADFTGIQE
                     FIFSIKSDGAARQLKGRSFAIQAMAIILQHELSSISGFELLYNAGGNLYFFVSDAEEA
                     KLGEFQSRIWDALSQES"
BASE COUNT          720 a          593 c          579 g          796 t
ORIGIN      
        1 tccatcacat attcctcctg tactgtccgc ctacttcgaa cagggcggtg gtgatttaaa
       61 aaaggaggga tgaactagtc acccctcccg gagacaaaac cattgtactt tctgttaaac
      121 gtattgtcat cctgcacagc ccagataatg gcatcaatta ctgaaatggc cgttggaacg
      181 aatgtccagc agaataacag atataacagt ccgtaccccg tttgccccaa gtagaacctg
      241 tgcactccaa gcccacccag gaggaaggcc agggctatgg ctgtccatct gtccttttct
      301 ttcattcggc aacgagttct cctctggtca ccaccggtgt gcctgaagaa aaatcaacga
      361 ccactctggc agacggtgct ccttcatggt aaggaaggat ctttgcggtc tgaccattgt
      421 attcaatgat aattggccgt ttgtctgttc ctgtataata ccagggtgtt ataaccggag
      481 tattataagg tgcagcagta taaacacctg tttccggatc cacaattttt atagaagctc
      541 ctttgggtat ggtactcacc gtgactttcc agccattcca ggtgatggat tgatcaacag
      601 gcggtagtgt cgatttagga tattccagca caccggtacc cttacaatga tcacaggtaa
      661 cttgtatctt tcctgttcca tcacatttgg aacacttata ggtcttgcca tctatcttac
      721 tgatataagc gccgtttccc gcacatttat cacacttata cagctggggg tctttgccgg
      781 taccagtaca tttgtggcat ctttctttgt gaattggtcg agatacatat tcgtaatgga
      841 cctcctcttc tttggtcact tctgtagatg aacattcaaa gaaccattca aagtttttct
      901 tctccgttac gtttccggct ttatcataag atacaggaac accgtttaag aacaggtaaa
      961 caacctgtgc attgaccagg ccatccagtt gtggaccatc aacgaaatac tctttttcaa
     1021 atccgctgag gatcaaacct ttattccatt cttccaaatg ggtggaagga aatgaccatt
     1081 gtgtgccgtc aatgatcagg gcaaaaccca cctcacttaa tttgagcggt acgtcctccc
     1141 ggtttctgat cctgatctta aaacgaatgt tttccggact cacccggtag tagggtgttt
     1201 tacgaatctc aaatacatca tagtcgtcct gtgccggtat cttgtaagca acattttctt
     1261 cctcttcttc catcaggtgt gcagagaaag agagtacttc cacagaaatg gtaactccgc
     1321 cttttgtttg ttcctgggtg gttttggcag cgggtttgac gacgggcaat gtgtattgta
     1381 cctgaaccgt ttcgtcagat ttttgtgtgt tctggtaggt tttttgcgtg taactcaatt
     1441 gctgtacggt gcaggatcct aaaagcaaaa gcaagcttag aatgattgtg gatggtaaca
     1501 agcgtttcat ggtgtggaag tttatcatag tgatatattt taattagtaa aaaagtcttg
     1561 tcagatagct gatcagaagc ataagccctg tccagatcag aaggccaacc ccgatgccgc
     1621 ccagaattcc cgtgatcagc cttctggtat tgttggaagg tatattggca aatgtttgca
     1681 ggctccaatc tgtcatcatg actcccagca ttagcaatga aacccaccag gcaggaatga
     1741 tccgggcaat gaccatcacc aatgctatga tatgaccgat gttggaacct gcacatcttg
     1801 cacatatgtg catgcgcctg ccattgatga ccaggcatct ttcaggtatg ccatggcatc
     1861 caagatgata agcagtgatc ttcacaggcc tgtcaggtcc atttattatt gcagttcaaa
     1921 caggtctttt cgatcttgtt ggctcctgat tgtccgaaca aaaatccaag cggaccacaa
     1981 gcaatgaacc cgcaacagcc tttagctgca ctgtagcctt ttttgtcaat atggatctgt
     2041 gtggaaccgc atttcggaca gacaacacgg tcttctgtgt gggtagtagt tgtcatacag
     2101 catcatttga tttagtatga caaagttttt actatgatct catatttgtc tgcttcaaaa
     2161 tctgattttg ggagcattat ttcttacagc aattgtgaaa taacagctat aaaaagctgt
     2221 attacaatgc tttataagga cacttttgag tataaagaat gctgtcctgt ggttttttca
     2281 agagaattat ttgaatatat ttgttgaaca agcatcctgt gaacacaaga tatgaattac
     2341 gaactgctct ttaaaaacta tcagcaaaag ctcaaaagtg catttgagaa ggatgcagca
     2401 ccacaaactg aacccagtac aggcaagctt ttagttaagg cagattttac cggtatccag
     2461 gagttcatat tcagcattaa aagcgatggt gctgccaggc aactgaaagg acggtccttt
     2521 gccatccagg cgatggccat catattacag catgagctta gtagcatctc aggttttgaa
     2581 ttgttgtata atgcaggtgg taacctctat ttctttgttt cagatgctga ggaggcaaaa
     2641 ttaggagagt ttcagtcccg catctgggat gcattatcac aagagagt
//
LOCUS       DAOMAX010000340         2684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8359_length_2684_cov_4.366679, whole genome shotgun sequence.
ACCESSION   DAOMAX010000340 DAOMAX010000000
VERSION     DAOMAX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_2684_cov_4.366679"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PK742_12525"
     CDS             complement(<1..389)
                     /locus_tag="PK742_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HQU77462.1"
                     /translation="MLRYIRLLMLVSLSGMVVETSAQVHLVVTSWPDEPESPEIYVAG
                     SFNSWQPGDELFRMQRTADGFVLDIPVRGTHEFKFTRGGWDKVETDASGKDVGNRNLS
                     GDGGTLVCSIAGWADGFAKKPPVLNEA"
     gene            526..>2684
                     /locus_tag="PK742_12530"
     CDS             526..>2684
                     /locus_tag="PK742_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQU77463.1"
                     /translation="MTVHAHAQEFMMQGWKWNYLQFLEGGGYLEYLENHAEELAEAGF
                     TYLWLPPLAEGSSGVASMGYDVKDYYNLGEYTSCRWGTRDELDALIATLNSHDIKAVA
                     DMVYNHRDGGSWEDNPSVEGWIENMNATKIAAGDQPFPSDRYRCYLPLGGASGNGAGT
                     YYFKIHSASGSGGFVGKPYTVMMWTNTVDANYGLAATAESEPNGGADCGEANDVITLG
                     KRFDAAVDGGCGTDELALTLEASDFNADGDTLWIRMYNTGSGGLGDMTDHFIYGLWSG
                     AAAEDIQDEIGYQTATNYGNVQSGQGYMDHTNFKPNGAPTQLAGDLDGMFFFYDVDQN
                     VASSRDVLFDNTVWNWNEVGIRGFRVDAVKHFPADFMGDLLDHLHDNGIDPGMVVGEA
                     YDFSAGYLNGWLNDVESNMDDDTKSAISVRVFDFALRNALEQASDAFGYDVRNVFNSG
                     IVDGAGGSGFHTVTFVNNHDFRDPGQPVDNDPMLGYAYILTNNKLGIPCVYHPDYYEP
                     LNLRYQINGLMEAHQRYIYGATQTDYLNRFSTPYASNYISGFPNTTLLYQLSYAESGR
                     EVIVVINYAGETLKLDHTINTANIFPGDTLTDIFAVTPYEYQIVSGDNQVYFEVPARS
                     FAVFVEGDLRDELIDISTPEEPENVLDIPVALQVFPNPADGYVHIEHTFTSQPDIRIS
                     DLQGKNLELPVTFQSGQLILNTQTLSPGRYFIHLQDG"
BASE COUNT          654 a          711 c          703 g          616 t
ORIGIN      
        1 atcgcctcgt tcaacaccgg aggttttttg gcaaagccat cggcccagcc ggcaatgctg
       61 cacaccaggg ttccgccatc gcctgataag ttgcggttgc cgacgtcttt tccgctagca
      121 tcggtctcca ccttatccca tccaccacgt gtgaacttga attcatgcgt tccgcgaaca
      181 ggaatatcca ggacgaaacc atcggccgtt cgctgcattc tgaacaactc atcacccggc
      241 tgccagctgt tgaaagaccc cgccacatag atctccggac tctccggttc atccggccag
      301 ctggtcacca ccaggtgcac ctgagcgctt gtttcaacaa ccatccccga cagggaaacc
      361 agcatcagta aacgaatgta tctcagcatg ttactgtatc aatttcgttt aaagttaagg
      421 ttagatgatt gaatgaaagg ccggaaattg agctctgctg tgtacatttg ctaactcttt
      481 tcaggcatga taagaaaaat gataatggca tgcatcctgc tgccgatgac tgttcacgcc
      541 catgcacagg agttcatgat gcagggatgg aaatggaact acctgcagtt tctggaaggc
      601 ggtggatacc tggaatacct ggagaaccat gcagaggagc tggcggaagc cggattcacc
      661 tacctgtggc ttccccccct ggccgaaggc agcagcggtg tggccagcat ggggtatgac
      721 gtgaaagact attacaacct gggcgaatac accagctgcc gctggggtac ccgcgatgaa
      781 ctcgatgccc tgatcgccac cctgaacagc catgacatca aggctgtggc cgatatggtc
      841 tataaccacc gcgatggcgg aagctgggaa gacaacccct cggtggaagg atggatagag
      901 aacatgaatg ccaccaagat cgctgcgggt gatcagcctt ttccgtcaga tcgctaccgt
      961 tgttacctgc cgcttggcgg agcttccgga aatggcgcag gtacctatta tttcaagatc
     1021 cattctgctt ccggttcagg cggatttgtg gggaagccct ataccgtcat gatgtggacg
     1081 aatacagtag atgccaacta tggtctggca gctacggccg aaagtgaacc gaacggcggg
     1141 gctgattgcg gggaagctaa tgacgtcatc accttaggca aacgttttga cgctgcggtg
     1201 gatggcggtt gcggtaccga tgaactggcg ctgacactgg aggcatctga ctttaatgcc
     1261 gatggcgata ccctgtggat acgcatgtac aacacgggct ccggcggact gggcgatatg
     1321 accgaccact tcatatacgg cctgtggagt ggcgcagcag ccgaagatat ccaggacgaa
     1381 ataggctacc agacggcaac gaactacggc aatgtccaga gcggacaggg ttatatggac
     1441 cataccaatt tcaaacccaa cggcgcacct acccagctgg ccggcgatct ggacggcatg
     1501 ttctttttct atgatgtgga tcagaacgta gcgtcttcca gagacgtgct gtttgacaac
     1561 acggtatgga actggaatga agtgggtatc cgtggtttcc gggtggatgc ggtcaagcat
     1621 tttccggccg acttcatggg cgacctcctc gatcacctgc acgacaacgg catcgatccg
     1681 ggcatggtgg tgggtgaagc gtatgatttc agcgcaggct atctgaacgg ctggctgaat
     1741 gatgtggagt ctaacatgga tgacgatacc aaaagtgcca ttagtgtccg cgtatttgat
     1801 ttcgccctgc gcaatgccct ggagcaggcc tcggatgctt tcggctatga tgtacgcaat
     1861 gtattcaaca gcggtatcgt ggacggtgcc ggagggtccg gttttcatac cgtcaccttt
     1921 gtgaacaacc acgacttccg cgatccgggt cagccggttg acaatgatcc gatgctggga
     1981 tacgcttata ttttgaccaa taacaagctc ggcattccct gtgtctatca tccggattat
     2041 tacgaaccgc tcaacctgcg ctatcagatc aacggactga tggaagcaca tcagcgctat
     2101 atctatggtg ctacccagac agattatctg aaccgcttca gtacacctta tgcgtccaac
     2161 tacatcagcg gttttccgaa taccacctta ctgtatcagc tcagctatgc agaaagcggc
     2221 agagaggtca tcgtggtgat caactatgcc ggcgaaaccc tgaagctgga ccataccatc
     2281 aacaccgcca acatttttcc cggcgatacc ctgaccgaca tctttgctgt gacgccatac
     2341 gagtatcaga tcgtgagcgg cgacaatcag gtgtattttg aagtgcccgc cagaagtttt
     2401 gcggtctttg tagagggcga cctgcgggat gaacttattg atatcagcac cccggaagaa
     2461 ccggaaaacg tcctggatat tcccgtagca ttacaggtat ttcccaatcc ggccgatgga
     2521 tatgtccaca tagaacatac cttcacttcg cagcctgaca tccgcatatc tgatctgcaa
     2581 ggaaagaacc ttgaacttcc tgttaccttt cagtcaggtc agctaatact gaacacacaa
     2641 acattgtcac caggccgtta tttcatccat ctgcaggatg gaga
//
LOCUS       DAOMAX010000341         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8368_length_2683_cov_3.987823, whole genome shotgun sequence.
ACCESSION   DAOMAX010000341 DAOMAX010000000
VERSION     DAOMAX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8368_length_2683_cov_3.987823"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK742_12535"
     CDS             <1..521
                     /locus_tag="PK742_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77464.1"
                     /translation="SCSRQVAKVAEGGLISGKTTAALQPDKLAGAKLSSPEPELATLT
                     EAYIPETDVRVSHEAVSAWNSYFDQDTTIVEEDTTPEPVMQPLILAGAATTGAGIAST
                     VLLFFGPWSLVLIPLLLIGGGVALTTFGYKKLKIEPEKYKGEKLARAVYYAMVGIGAV
                     AMLIPVWLLFNL"
     gene            complement(526..1092)
                     /locus_tag="PK742_12540"
     CDS             complement(526..1092)
                     /locus_tag="PK742_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77465.1"
                     /translation="MWRWMITGLLLFPAMVHAQLFTSYTGDGSPGEILTNFDQTPVFP
                     GDQPELFNYFETNVYPNMPSRSQQDVNLAEGTGSFPEEKVVLAFDLNRYGEVSNRRIE
                     YNSNLLMERSWQRALDKMPIWAPAICYGEPTDVQVYLPLRYVISDNQVVILGWGEWLY
                     ESSGRNLWLKVALGVAVLATFTYLLFGQ"
     regulatory      1151..1357
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1449..>2683
                     /locus_tag="PK742_12545"
     CDS             1449..>2683
                     /locus_tag="PK742_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77466.1"
                     /translation="MKRTFLFLSLLWAFTAFAQESLHQILILNEGYTDYLTGEIIEPV
                     SVGSIDPDTYTYTELFTVDGARFASDLVIDGDRFFVAADDQLLSYDLNTYELLASAEV
                     AGIRKIAIWNDQVIVTRGEYLVTLDSHVEVFGREDLNFLYAINTDDIPYTTEGIAVID
                     DKAYVAVNNGFDFGNEVGQIAVIDLNSEMLETTVELGADGINPDNLMVEDGIIYTLNN
                     KDFTGSSVSTYSPASGTVATVNLMNISSGCGTSTLREGLVYYQEMFGTSISRFDPASE
                     LIVDENEFGYSFYALSFEPVSGTMMATTTDFFSTGSFLVFDDFGAVAHEVAVGVSPGT
                     IAFDVRNASAIQEKEIGTLRLYPNPASGSIVVSGNGMLRIYNTTGQLVDEQTIVSGQA
                     DVSALIAGLYTLQLEQDGV"
BASE COUNT          658 a          655 c          686 g          684 t
ORIGIN      
        1 gctcctgttc gcgtcaggtg gcgaaagtgg ctgaaggtgg attgatatca ggaaagacca
       61 ccgctgcctt gcaacctgat aaattggcgg gagcaaaact ttcttctcct gagcccgaac
      121 tggctactct gacagaagca tatattcctg aaactgatgt acgagtttca cacgaggctg
      181 taagtgcctg gaacagttat ttcgaccagg atacgaccat tgtagaagag gataccaccc
      241 cggagccggt catgcagcca ctgatcctgg ccggtgctgc aacaaccgga gcaggcattg
      301 caagcactgt gctgttgttt ttcggtccct ggtcgctggt gctcatacca ttactgctga
      361 ttggcggagg tgtggcactg accacctttg gttataagaa gctgaagatc gaaccggaga
      421 agtacaaagg tgagaagctt gccagggcag tttattacgc catggtaggt ataggcgcag
      481 ttgccatgct gatccctgtt tggttgctat ttaatctgtg aaaatctact gcccgaaaag
      541 cagataggta aacgtagcca aaacagctac acccaaagcc actttcagcc ataggttcct
      601 cccgctgctc tcatacagcc attctcccca tcccagaatg accacctggt tgtcgctgat
      661 cacataacgc agcggcagat atacctgcac gtcggtaggc tcaccgtagc agatggctgg
      721 tgcccagatg ggcattttgt ccagtgccct ttgccaggat ctttccatca ataagttgct
      781 gttgtattcg atccttcggt tgctcacttc tccgtatctg ttgaggtcaa aggccaggac
      841 aaccttttct tccggaaagc ttccggtgcc ttcagccagg ttcacatcct gttgcgagcg
      901 ggaaggcatg ttgggataca cattcgtttc aaaatagttg aaaagctccg gctgatcgcc
      961 gggaaagacc ggggtctgat caaaatttgt gaggatctct ccggggctgc cgtcaccggt
     1021 ataagaggta aaaagctgag catgcaccat cgccggaaag aggagtaatc ctgttatcat
     1081 ccatcgccac ataccacaaa taacgccaat gttgaaaaat aagttgaaca gcagaataag
     1141 atgtatttac ttttgatccc gttgaaacgg ttgatctgtc ctgtttcagg acgatcctaa
     1201 gagggaatcc ggtgtaattc cgggacagtc cccactgctg tgatccttac ccttcgggga
     1261 agtttccggg ccaatctcca gccactgccg ggcatccggt gggaaggcgg ccggaaagag
     1321 gaaagtcaga agacctgccg catcaatgtt ttcaaaactt ttggggcaaa agtggagaac
     1381 aaagaggtag ccagacctat ttgccatccg ttttttcctg ctttatggaa ccttttaaaa
     1441 caattggcat gaaaagaact ttccttttcc tttccctcct gtgggcattc acagcctttg
     1501 cacaggaatc actacatcag atcctgatcc tcaatgaagg gtatacggat tatctgacgg
     1561 gcgaaatcat cgaacccgtt tctgtgggca gcatcgatcc ggacacctac acctacacag
     1621 aactgttcac ggtggacggt gcacgttttg catctgacct cgttatcgat ggtgatcgtt
     1681 tttttgtagc agccgatgat cagctgctga gttatgatct gaatacctac gaactgctgg
     1741 cttctgcaga agtagccggt atcaggaaga tcgccatatg gaatgatcag gtgatcgtta
     1801 cccgtggtga atacctggtt acccttgaca gtcatgtgga ggtgttcggc cgggaagatc
     1861 tgaacttcct gtatgccatc aacacggacg atattcctta taccaccgaa ggcattgctg
     1921 tgatagacga caaagcctat gtggccgtca acaacggttt cgattttggc aatgaggtgg
     1981 gacagatcgc ggtcatcgac ctgaacagtg aaatgctgga aactacagta gaattaggag
     2041 ctgacggcat caatccggat aacctgatgg tggaagatgg catcatctac accctgaaca
     2101 acaaggattt tacgggcagt tctgtgtcca cctattcacc ggcgtcaggc acagttgcta
     2161 ctgtcaacct gatgaacatc tcttccggtt gcggcacttc caccttgcgt gaggggttgg
     2221 tgtactacca ggaaatgttc ggtacttcca tcagccgttt tgacccggct tctgaactca
     2281 tcgtagatga gaatgaattc ggatacagtt tttatgcctt gtcgtttgag ccggtaagcg
     2341 gaaccatgat ggccaccacg accgattttt tcagcaccgg cagcttcctg gttttcgacg
     2401 atttcggtgc tgttgcacat gaggtagctg tgggagtttc tcccggtacc atcgcatttg
     2461 acgtgcgcaa tgcatcagct attcaggaga aagagatcgg gacactgcgt ctgtatccca
     2521 atcctgcaag tggttcgatt gtggtatccg gcaatggtat gctgcgtatc tacaatacca
     2581 caggacaact ggtagatgaa cagacgatcg tttccggtca ggcagatgtg tctgcactca
     2641 tagctggtct gtacaccctg caactggaac aggatggtgt gat
//
LOCUS       DAOMAX010000342         2676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8408_length_2676_cov_4.413964, whole genome shotgun sequence.
ACCESSION   DAOMAX010000342 DAOMAX010000000
VERSION     DAOMAX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8408_length_2676_cov_4.413964"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK742_12550"
     CDS             complement(<1..267)
                     /locus_tag="PK742_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQU77467.1"
                     /translation="MFPEYDVIVVGAGHAGCEAAAAAANMGSKVMLVTMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVTDETAIQFRMLNRSKGPA"
     gene            complement(331..786)
                     /gene="ybeY"
                     /locus_tag="PK742_12555"
     CDS             complement(331..786)
                     /gene="ybeY"
                     /locus_tag="PK742_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQU77468.1"
                     /translation="MSQPISFHSLVDGFSIKGKKSLRSWLERVISAHHFVPGEISIIY
                     CSDNYLAEMNQDYLQHDTFTDIITFNYNVGSQVSGDIFISIDRVAENAASFGATLENE
                     LCRVMVHGVLHLLGYGDKTPDEEHAMRSAEDHFLSWLEEKPRVSRETSA"
     gene            complement(783..1199)
                     /locus_tag="PK742_12560"
     CDS             complement(783..1199)
                     /locus_tag="PK742_12560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQU77469.1"
                     /translation="MATTEAVVDKELSLPSHPASVVQVESFIEDFRSLHQICDEVYGN
                     MLVAITEAVNNAIIHGNQTDQQKQVTIHLQCRRNLVTCTVQDQGGGFDFNHVPDPTAP
                     ENLEKEGGRGIFLMKHLSDLVVFSNDGSQVEIQFRL"
     gene            complement(1335..2297)
                     /locus_tag="PK742_12565"
     CDS             complement(1335..2297)
                     /locus_tag="PK742_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77470.1"
                     /translation="MKKHYLIACILFVVSSVQAQLPNYFIQPDLHEETVMETFVLKGG
                     ENSKQSDSLVWVKVQTDTLKSSNDTLRFFLYGSAAINTTEDLQTSLSASQQFAALFRV
                     LPFQPKENKNSGNGLDVIISMNALHLRPTGVAADSIDLESMLFPQTGNAGFIVGPRYQ
                     YFGKNNGAVYHSLSGSAAFALRQARVTGVEVTDTSGVLTSRDIKVDVINLNIMPVEWT
                     FAYSREDLKCSFSAGLYYHWFNIPNEDAASFNLLMDEDVFRESGRNSFINALGMRFRG
                     EINSFEFFTDIRYNSTGRTFSNDNPYKGFVYNAGFSTNLIIFSK"
     gene            2464..>2676
                     /locus_tag="PK742_12570"
     CDS             2464..>2676
                     /locus_tag="PK742_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77471.1"
                     /translation="MKIITQVFCLGLLVAFGLQACTEQDSKENGGDQISQESINQDKS
                     NGIKKNLATRITRYAMENYKLGSSGIA"
BASE COUNT          736 a          614 c          608 g          718 t
ORIGIN      
        1 ggcagggcct tttgatctat tgagcatcct gaactggatg gcggtttcgt cggtcacgat
       61 gccagaataa ccacctaatg catcgatctc acgaacgatc tgcccttttg ccacaccacc
      121 catagctgga ttgcatgaca tctgggctat ggtttgcatg ttcatagtaa cgagcatgac
      181 tttagagccc atattggcag cagcagcggc cgcctcacaa ccggcatggc cggcacccac
      241 tactatgaca tcatattctg gaaacatgcg gtgacaaaat tacatcaagg caaactgtga
      301 tttgttccac gtggaacata taggcgaaga tcaggcagat gtttcacgtg aaacacgagg
      361 cttctcttcc agccaggaaa gaaaatgatc ttctgcgctg cgcatggcgt gctcttcgtc
      421 aggtgttttg tctccgtaac ccagcagatg taaaaccccg tgtaccatga ccctgcacag
      481 ctcattttcc agagtggcgc caaatgatgc agcattttca gccacccggt cgatgctgat
      541 aaatatatcg ccggatacct gactgcctac attgtaatta aaggtaatga tgtctgtaaa
      601 ggtgtcatgc tgcaggtagt cctggttcat ttcagccaga tagttgtcag agcaataaat
      661 aatggaaatc tctcccggaa cgaagtgatg agcagaaatt acacgttcca gccagctgcg
      721 caggctcttt ttccccttga tcgagaagcc atccacaagt gagtggaaac tgatgggttg
      781 gctcataatc gaaactgtat ctctacctgg ctgccatcgt tggaaaatac caccaggtca
      841 ctcaggtgtt tcatcaggaa aattccccga ccaccttcct tttccagatt ctcgggtgcg
      901 gtggggtctg gtacgtgatt gaaatcaaaa ccaccaccct gatcctgtac ggtacaggta
      961 accaggtttc tgcggcattg caaatgtatg gtaacctgtt tttgctgatc tgtctggtta
     1021 ccgtgaatga tggcattgtt gaccgcctcg gtaatggcta ccagcatatt accatacacc
     1081 tcatcacaga tctggtgaag acttctgaag tcttctatga acgattccac ctgaactacg
     1141 ctggcgggat gagatggaag cgaaagttct ttatccacca ctgcttctgt tgttgccatg
     1201 aaaaaagcta tgcgttagca tactctttta ctgattccgg acaaaaaagt ttcaattggt
     1261 gacagggttt attaaggtgt ataacaaaaa ccggatacac ctgatgtatc cggttcctgg
     1321 ttgaaggcat tggcctactt gctgaatatg atcagattgg tggagaaccc ggcattatat
     1381 acaaagcctt tgtagggatt gtcgttagaa aaagtccttc ctgtgctgtt atagcgaata
     1441 tcagtaaaga attcaaagct attgatctcg ccacgaaaac gcattccaag ggcgttgatg
     1501 aaactgttcc tgccactttc gcggaacaca tcttcatcca tcagcagatt aaaagaggct
     1561 gcgtcttcat tgggaatatt gaaccagtgg taataaagac cggcactgaa agaacactta
     1621 aggtcttcgc gtgagtaggc aaaggtccat tcaaccggca tgatgttcag gttaatgaca
     1681 tctactttga tgtctctgga tgttaatacc ccggatgtat ctgttacttc caccccggtt
     1741 acccgtgcct gtctgagtgc aaaggcggca ctgcctgaca gtgaatgata cacagctcca
     1801 ttgttcttac caaagtactg atatctcggc cccactatga acccggcatt ccctgtttgt
     1861 gggaacaaca tggattccag atctatggaa tctgcagcta cacctgttgg cctgagatgc
     1921 agggcattca tggaaatgat cacgtccaga ccgtttccac tgttcttgtt ttcttttggc
     1981 tgaaaaggca agaccctgaa tagtgctgca aactgctgag aagcagacag tgaagtctga
     2041 aggtcttctg tagtattgat ggcagcagac ccgtacaaga agaaccggag ggtatcattg
     2101 gagcttttga gtgtgtcggt ctgcaccttg acccaaacaa gagaatctga ctgttttgaa
     2161 ttttctccac cctttaacac aaacgtttcc atcaccgtct cttcgtgcag gtctggctgt
     2221 ataaagtaat tgggtaattg tgcctgaacc gatgatacaa caaatagaat acacgcaatc
     2281 aggtagtgct ttttcatgat gattttttga tattataaat cgtttaagac cacattgatg
     2341 atgcaaacat atttccggaa accgactaca ggcagggtat ttacaccccc gctatagtca
     2401 cctgaataat agcagagctt atttctactt ttgtgcagca tctaatacat aaaacgttga
     2461 cacatgaaaa taattacaca agtgttctgc ctggggttgc tggtggcctt tggtttacag
     2521 gcatgtacag aacaagactc aaaggaaaac ggaggtgacc aaatctctca agaaagtatc
     2581 aatcaggaca aatccaacgg aattaaaaag aatttggcaa ccaggatcac ccggtacgca
     2641 atggaaaatt ataagctggg tagttcaggt atagcc
//
LOCUS       DAOMAX010000343         2660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8481_length_2660_cov_4.805374, whole genome shotgun sequence.
ACCESSION   DAOMAX010000343 DAOMAX010000000
VERSION     DAOMAX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8481_length_2660_cov_4.805374"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK742_12575"
     CDS             complement(<1..686)
                     /locus_tag="PK742_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77472.1"
                     /translation="MNERLYKWIYVLMATAIFVLILIKVFQVPITHDETATTVHYVHY
                     NAWEIMMFPDNWPNNHILNTLLVKYSIALFGAHSWSVRLPNLLSFLLFAWAVYRIACL
                     LNPRSFWLPVTAVLFFANPYLLDFFGLSRGYGLSVALEMTAISFLLSGICHQKILHLF
                     LAAVISCFSAYASFTLLPWWASMFLLLLLAIWKLDTKRKWQNSLWLAALGLGFLALIA
                     TPLYKMQSTD"
     gene            complement(679..2460)
                     /locus_tag="PK742_12580"
     CDS             complement(679..2460)
                     /locus_tag="PK742_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77473.1"
                     /translation="MQYFSLNSRNTLMLLYSLLLAVHIFFYPKWRETGTETTISWDVS
                     GYYMYLPAIFIYHDLEQVAFLDSISKTYQPAGDDYQAFQIGNGNRVMKYTMGQAIMYS
                     PFFFLGHAWALADNRFPADGFSKPYQVAISLSSLVFALIGLWYLRKLLLLFFADKAVA
                     WSLLVIVTGSNYLNYSAIDGALTHNYLFTLYVAVLWTTHQFWRSPNMHSAMLIGFWCG
                     LAALTRPTELILIFWPVFWGLSAWDRSGIRQRFREIIRQKKYWSGMVLTALVIGSMQL
                     IYWRSVTGQWLFYSYEDQGFSWLEPHIIKGLFSYRSGWLVYSPAMLLCLIGFLFLLKK
                     NRSFGISAVIYTVLFVYIAFSWDIWWYGNSLGQRAMIQLYPVLAFPLAAFFESCVWHR
                     TWSAVLLSLFLFITVYLNGWFTYQAHKGGLLPTGINNRQYFWRTVGRFHQDPNDIKLL
                     DYSEVYRGSMEQADLILDTSETSQWNSGPIVLLQDDTVTNEWMLAVPSDNPDWMRIYF
                     RATVPYREPDIWKQDQLLIRYHVQGRMDHQESIRIQRLMQENTWPLYMDVQFNRYQAD
                     SIGVMFKHNLSVYPLTIDSILIYSIHE"
BASE COUNT          841 a          607 c          595 g          617 t
ORIGIN      
        1 tgatcagttg attgcatctt atacaatggc gtagctatca gtgcgagaaa tcccagaccc
       61 aaagcagcca accacaaact attctgccat ttcctctttg tatccaattt ccagatagct
      121 agaagcaaca acaggaacat agaagcccac caggggagca aagtaaaact tgcatatgca
      181 gaaaaacagg agatcactgc agccagaaac aggtgaagga tcttttgatg acaaattcca
      241 gacaacagaa aactgatcgc tgtcatttcc agagctactg acaagccata gcctctggac
      301 aatccgaaaa aatccagcag atatggattg gcaaagaaca atacagcagt gacaggaagc
      361 cagaaagatc tggggtttaa cagacatgct attctgtaaa cagcccaggc aaacaacaga
      421 aaggaaagta gattgggtaa tctgaccgac cacgaatgcg ctccgaacaa tgcaatgctg
      481 tacttgacga gaagtgtatt gaggatatgg ttattaggcc agttgtcggg aaacatcatg
      541 atctcccacg cattgtaatg cacgtagtga acagtggttg ctgtttcatc gtgcgtaatg
      601 ggaacctgaa agaccttgat caggatcaga acaaaaatgg ctgttgccat caacacatag
      661 atccatttgt ataaacgctc attcatgaat ggaatatatc aggatggaat cgatggtgag
      721 tggatataca gacaaattat gcttaaacat aacaccgata gaatcggcct gatacctgtt
      781 gaactgcaca tccatgtaca gcggccaggt attttcctgc atcagtcgct ggatacggat
      841 ggattcctgg tgatccattc taccttgaac atgatatctt ataagtaatt ggtcctgctt
      901 ccaaatgtct ggctcccggt aaggtacagt ggcccgaaag taaatgcgca tccaatccgg
      961 attatctgat ggtactgcca gcatccattc attggtcacc gtgtcatcct gcaatagaac
     1021 aattgggccg gaattccatt gactcgtttc acttgtatcc agtataagat ctgcctgctc
     1081 catggagcct ctgtaaactt ctgagtaatc cagaagttta atatcgttag gatcctgatg
     1141 aaatcttccc acagtacgcc aaaaatactg cctgttattg atgccggttg gcagaagacc
     1201 tcccttatgc gcctgatagg tgaaccagcc attgagataa acagtaatga acagaaataa
     1261 cgaaagcaac acagctgacc acgttcgatg ccagacacaa ctttcaaaaa atgcggccag
     1321 cggaaacgcc agtaccggat acaactgaat catggcccgc tgcccaagtg aattaccata
     1381 ccaccagata tcccaggaaa aagcaatgta aacaaacaac acagtataga tcacggcaga
     1441 gataccaaag gatctgtttt tcttcaagag gaataagaat ccgatcaggc acagcaacat
     1501 tgccggtgaa tagaccagcc aaccgcttct gtagctgaac aaccctttga tgatatgcgg
     1561 ctcaagccag ctgaaaccct gatcctcata agaatagaac agccattgac ccgtaaccga
     1621 acgccaatag atcaattgca ttgatccaat gacaagtgcg gtgagcacca tacctgacca
     1681 atattttttt tgacggatga tctcgcggaa acgctgacgg attcccgatc tatcccaggc
     1741 agacaatccc caaaaaaccg gccagaagat cagtatcaat tcagtgggtc tggtcagggc
     1801 cgccaatccg caccaaaaac ctatcagcat ggcagagtgc atgtttggtg atcgccagaa
     1861 ctgatgggtg gtccacagaa ccgccacata aagggtgaac aggtagttat gggtcagggc
     1921 gccatcaatg gctgaatagt tcaggtaatt agatcccgtc actataacaa gcagcgacca
     1981 ggcaacagct ttatcagcaa aaaaaagcag gaggagtttt cgcagatacc agaggccgat
     2041 cagagcaaat accagactac tcaatgagat ggccacctga tagggcttag aaaagccatc
     2101 agccggaaac ctgttgtctg ccagagccca ggcatgtccc agaaagaaaa aagggctgta
     2161 catgatagct tgccccatcg tatatttcat aacccggttg ccgttcccga tttggaatgc
     2221 ctgataatca tcacctgcgg gctgataggt tttcgatatc gaatccagaa aggctacctg
     2281 ttccagatca tggtagataa aaattgccgg caggtacata tagtagcccg atacatccca
     2341 ggagatggtg gtttctgtac ctgtttccct ccatttagga tagaaaaaga tgtggaccgc
     2401 cagtagcagg ctgtacaaca acatcaatgt atttcttgaa ttcagggaga aatactgcat
     2461 gcaaggttta ttggccatcc aaatatgacc tttttataat atttactgcc gaaggggttt
     2521 aatataccct gcaatctgac gaaaaaatgt tttggtagct tagtcttggc ttttcaaggt
     2581 gagggaacga accatagaaa aacaagatcc cgagaggaac gattcgggat tggtatttga
     2641 aattatggtg cggtagctca
//
LOCUS       DAOMAX010000344         2655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8510_length_2655_cov_4.088846, whole genome shotgun sequence.
ACCESSION   DAOMAX010000344 DAOMAX010000000
VERSION     DAOMAX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8510_length_2655_cov_4.088846"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..765)
                     /locus_tag="PK742_12585"
     CDS             complement(7..765)
                     /locus_tag="PK742_12585"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HQU77474.1"
                     /translation="MLRKPTFLFLFLTLCGTAASAQIINIEEYRVRTDSVGWTGSGEA
                     SAYISKNDDFVFSLLTNIQIQHKRPKTTWWLITDLETVQANGQDAFVNAGFEHLRLNY
                     RINERWVWEAFAQGVFNEPLGVDWKLLAGTGPRWKVVEKDQFRLYLAALYMFENEKNP
                     ESADPVQFHRMSSYASFTWELKNKTRFSNTTYYQPVLNDVADYRIASNTSLKAFMTKM
                     LYLELEYNLYFDTRPPTGIVTTVYNLQSGIGLEF"
     gene            980..1396
                     /locus_tag="PK742_12590"
     CDS             980..1396
                     /locus_tag="PK742_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77475.1"
                     /translation="MQKSYVYSCMPNSFVLRSWTIAGAFYMLLVSVSHLLYIKVPGLF
                     VFPDTASYIYQDRLIGMLALGWSFWLWTGIKNPESIRLLPFAGLISVITIWSEVLFFP
                     DLIDQGYQLPQYLAACLQTIYVGGLWFLLRRHFSVS"
     gene            1436..1523
                     /locus_tag="PK742_12595"
     tRNA            1436..1523
                     /locus_tag="PK742_12595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1470..1472,aa:Ser,seq:tga)
     gene            complement(1812..>2655)
                     /locus_tag="PK742_12600"
     CDS             complement(1812..>2655)
                     /locus_tag="PK742_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQU77476.1"
                     /translation="DVWKFFLNEIRRDVLPLRSAAISFNFLLAIFPGIIFLFTLIPYI
                     PVRDLNVTILDMFNQVLPESGFEFLSETINDIVSIKRGGLLSFGFVLAFYFSTNGVRM
                     IMLAFNKEHPIYNRRGFWRRRVASVRLTLYLFLLFMASVLLIIAGDHVMERIQSQSGL
                     KDATWYWIFNIIKWFFLIMLFFVTISMIYYYGPAVRVRRRFITPGGSFATFSSIIASI
                     GFGYFVNNFGMYNQVYGSIGTLIVLMLWLNLNALVLLVGFEINNSIDVNHVLRMKEKE
                     KAGE"
BASE COUNT          734 a          638 c          604 g          679 t
ORIGIN      
        1 ttgttctcag aattccagac caattccact ttgcaggtta tatacagtgg tcacaatgcc
       61 cgtgggcgga cgtgtatcaa aatacaggtt gtactccagt tccagataga gcattttggt
      121 catgaatgcc ttcagactgg tgttggatgc aatgcggtaa tcagctacgt cattcaacac
      181 cggctggtag taggtagtat tgctgaagcg ggtcttgttc tttagttccc aggtgaatga
      241 tgcataggaa ctcattcggt gaaactgcac aggatcagca gattccgggt ttttttcgtt
      301 ttcaaacata tagagcgcag ccagatacag acgaaactga tccttctcca cgaccttcca
      361 ccgcggacct gttcctgcaa gcagcttcca gtcaacaccc agcggttcat tgaatacccc
      421 ctgggcaaag gcttcccaaa cccatctttc attgatgcgg tagttcaaac gcagatgttc
      481 aaaacctgca ttcacaaagg catcctgccc gttggcctgc accgtttcga gatcggtgat
      541 cagccaccag gtggtcttcg gtcgcttgtg ctggatctgg atattggtca gcaaggaaaa
      601 gacgaagtca tcgttcttac tgatataggc agaagcttcg ccgctgcctg tccagccgac
      661 agaatctgta cgaacacggt attcttctat gttgatgatc tgggcagagg ccgctgttcc
      721 gcataacgtg aggaacagga acaggaaggt gggttttctt agcatgcagc taaaatactc
      781 caaaagaaaa aaggcagcca tcagcacagg acttgtgatc ctgtgcttag gctacccccg
      841 ccacggttat aatctgaatt ttcttattgt catacgaatg taagccaatt cctgaattgt
      901 tcaaaactaa atgatggaat cttgtactac tactgcaaca gggaaactgt gttttccact
      961 tttctgccag aaataaatca tgcagaaatc gtacgtttac agctgtatgc ctaacagctt
     1021 tgttctgagg tcatggacca ttgccggagc attctatatg ctgctggtat cagtcagtca
     1081 tttgctgtac atcaaagtac ccgggctgtt cgtttttcct gacactgcct cttatatcta
     1141 tcaggacagg ctcataggca tgctggcctt aggttggtct ttctggttat ggacaggtat
     1201 aaaaaacccc gaatccatca ggctcctgcc ttttgccggt cttatttctg tgatcaccat
     1261 ctggtcagaa gttctgtttt tccctgattt aatagatcag ggctaccagt taccccagta
     1321 tctggcagcc tgcttgcaaa ccatttatgt tggcggcctg tggtttctcc tgcgccgtca
     1381 tttttctgtt tcctgaagtc ctttcattca tcagacggtt ttctatcttt gcgccggaga
     1441 gatggcagag cggtcgaatg cggcggtctt gaaaaccgtt gagggtaaca cctccggggg
     1501 ttcgaatccc tctctctccg ccaacaagat atgccccgca atttgcgggg ctttttattt
     1561 tccggcagag cgcccggatg tatccggggc gtgacgccgg gaaataaaaa ccggaccgga
     1621 ggaacgggca gatcgggttt gatacccatc atcaaaggga tcgcggcagc aatcccgtaa
     1681 aaggtataag ggtgcaaaaa atgtagctta aaaggtaaaa gttgcaagag aaaagcagat
     1741 cggttaccct caaagcccca aacgaacaac gaataacgaa caacgaacaa cgaataacga
     1801 ataacgcaca cctactctcc tgccttctcc ttctctttca ttcgcaacac atggtttaca
     1861 tcaatgctgt tgttgatctc aaatcctaca agcaatacca gggcattcag attaagccag
     1921 agcattagta caatgagcgt tcctatggag ccatatacct ggttatacat accaaaatta
     1981 ttgacgaagt acccgaatcc tatggaagcg atgatgctgg aaaaggtggc aaaacttcca
     2041 cctggagtaa tgaaccgtcg ccgcacccgt acagccggtc cgtaataata gatcatggaa
     2101 atggtcacaa aaaaaagcat gatcagaaag aaccacttaa taatattgaa tatccagtac
     2161 caggtggcat ctttcagacc cgactgactc tgtatgcgtt ccatcacatg atcgccggca
     2221 atgatcagca gtacagaagc catgaacaac aggaacaggt agagtgtaag cctgacagaa
     2281 gccactcttc tgcgccagaa tcctctgcgg ttatagatcg ggtgttcctt gttaaaagcc
     2341 agcatgatca tacgcacacc gtttgtgctg aaatagaaag ccagcacaaa accgaatgac
     2401 aacaaccccc ctctcttgat actgacaatg tcattgatgg tctctgaaag aaattcaaaa
     2461 ccgctttcgg gcaatacctg gttgaacata tccagaatgg ttacattcag atctcgaacc
     2521 ggtatgtaag ggatcaaggt gaacaggaat atgataccgg gaaagatggc caggaggaag
     2581 ttgaaagaga tggctgcaga acggagcggc aatacatctc tcctgatctc attcagaaaa
     2641 aatttccata catca
//
LOCUS       DAOMAX010000345         2647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8552_length_2647_cov_3.451775, whole genome shotgun sequence.
ACCESSION   DAOMAX010000345 DAOMAX010000000
VERSION     DAOMAX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8552_length_2647_cov_3.451775"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="gdhA"
                     /locus_tag="PK742_12605"
     CDS             <1..1180
                     /gene="gdhA"
                     /locus_tag="PK742_12605"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQU77477.1"
                     /translation="EPERVIIFRVPWVDDKGDVQINRGYRIQMNTAIGPYKGGLRFHP
                     SVNLSILKFLAFEQVFKNSLTTLPMGGGKGGSNFDPKGKSDMEVMRFCQSFMSELYRH
                     IGPNTDIPAGDIGVGGREIGFLFGQYKRLRNEFTGVLTGKGQSWGGSLIRPEATGYGC
                     VYFAEEMLNQKNDSLKGKTVAISGSGNVAQYAIEKCLDLGAKAVTASDSSGYIYDKNG
                     FDREKLAYLMDLKNVRRGRIEEYAKRYKCEFFPMKRPWGVKVDVALPCATQNELNEKD
                     AATLVKNGVLCVAEGANMPSTPEAIYVLQQAKVLYAPGKASNAGGVATSGLEMSQNSI
                     RQNWTREKVDEELRQIMHNIHKTCLQYGKERGHINYVKGANIGGFMKVADAMLAQGIV
                     "
     gene            1292..>2647
                     /locus_tag="PK742_12610"
     CDS             1292..>2647
                     /locus_tag="PK742_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQU77478.1"
                     /translation="MESFFKILKSLRRLAGFLLPLVSVIGVIILLYDFGFEHTPETAA
                     LLVRFYDYFLFVVFMLLLLRVDYAGLIRKKVKYTKTELALFFIFAFLWLGQFPLAGLF
                     SEKSLFHRILDLYIIDVALVVLLFLAELSLGSLNMSRYNLNPALVFLASFVLIILIGT
                     ILLLLPNSTYQGIGLTDALFTSTSAVCVTGLIVVDTATYFTPIGQNIILVLIQTGGLG
                     ILTFTSFFGIYLRTSSSFQNQLMIGDMLNEDRISDVFKTIVRVIVFTFLVEALFAVFI
                     YFTLDERQFPSHSEMIRFSVFHSVSAFCNAGFSLLSDGLYDVHYRFNYPFLLIISFLV
                     ITGGLGFNIIFNFFRYLKHRLIRLSKQLFTRNVVQYKARIIEVHTKLVIITTTFLLVF
                     GFIMNLVLEYHNTLEEHGTFGKLVTAFFASVTPRTAGFNTIDMGALLPATILIYFLLM
                     LI"
BASE COUNT          626 a          633 c          673 g          715 t
ORIGIN      
        1 tgaacctgag cgtgtcatca ttttccgggt tccctgggtg gacgacaagg gggatgtaca
       61 gatcaatcgc ggataccgta ttcagatgaa taccgccatc ggaccctata agggcggact
      121 tcgtttccat ccgtcggtca acctcagcat cctgaaattt ctcgctttcg aacaggtgtt
      181 caagaacagc ctcactaccc tgccgatggg tggtggaaag ggtggttcca acttcgaccc
      241 caagggtaag agtgatatgg aagtaatgcg cttctgccag agcttcatgt cagaactgta
      301 caggcatatc ggaccgaaca cggatattcc tgcgggagat atcggtgtag gtggacgtga
      361 gatcggcttc ctgttcgggc aatacaaacg gttgcgcaac gagttcaccg gtgtacttac
      421 cggcaaggga cagtcatggg gtggaagtct cattcgtccg gaggctaccg gttatggctg
      481 tgtgtatttc gcagaagaaa tgctgaacca gaagaacgac agcctgaaag gaaagaccgt
      541 agccatcagt ggttccggca acgtggcgca gtatgccatt gaaaaatgcc tggatctggg
      601 agccaaagct gtaactgcca gcgacagttc aggttatatc tatgacaaaa atggcttcga
      661 tcgcgaaaag cttgcatacc tgatggatct taagaatgtg cgccgtggtc ggatagaaga
      721 atatgccaaa cggtataagt gtgagttctt cccgatgaag cgtccgtggg gtgtgaaagt
      781 ggatgtggct ctgccatgtg ccacccagaa tgagctgaac gaaaaagatg cagccaccct
      841 ggtgaagaac ggtgtgctgt gcgtggccga aggtgccaat atgccatcca ctccggaagc
      901 catctatgtg ttacaacagg cgaaggtgct gtatgcaccg ggcaaggcca gcaatgccgg
      961 tggagtggcc accagcggac tggaaatgag ccagaacagc atacgccaga actggaccag
     1021 agaaaaggta gatgaggagc ttcgtcagat catgcataat attcacaaga cctgtctgca
     1081 gtacggaaaa gaaagaggcc atatcaacta tgtgaagggt gccaacattg gcggattcat
     1141 gaaggtggcg gatgccatgc tggcacaggg aattgtatag gaaggaaaaa atgggctgat
     1201 cagagatacg gcactgccgt gcatgctttg gtcagctcac tatactattt cctaaatatc
     1261 accttacttc ctgtacattt gtaccgcacc cttggagagt tttttcaaaa tactgaagtc
     1321 gctccgcagg ctggcgggtt tcctgctgcc tcttgtaagt gtcataggtg tcatcatcct
     1381 gctctacgat ttcggatttg aacatacacc ggaaacggct gcacttctgg tcaggtttta
     1441 tgactacttc ctctttgttg tttttatgtt gctgttactg cgggtggact atgcagggct
     1501 gatccgcaaa aaggtaaagt acacaaaaac ggaactggca ctgttcttca tctttgcatt
     1561 tctgtggctg ggtcagtttc cattagctgg attgttctct gaaaagagcc tgtttcacag
     1621 gatcctggat ctgtacatca tagatgtggc tctggtggtg ctgctgttcc tggcagaatt
     1681 gtcgctgggt tcacttaata tgtcccggta caacctgaac ccggccctgg ttttcctggc
     1741 cagtttcgtc ctgatcattc tcattggcac catactgtta ttgcttccca actcgaccta
     1801 tcagggcata ggtttgacag acgctttgtt cacttccact tctgctgttt gtgtgaccgg
     1861 gctcatagtg gtggatacgg ccacttactt cacgcctatc ggacagaaca tcatcctggt
     1921 actgatccag accggtggac tgggtatcct gacctttact tcctttttcg gcatctatct
     1981 gcgcaccagt tcgtcttttc agaaccagct catgatcgga gatatgctca atgaggatcg
     2041 catcagcgat gtgttcaaga ccatcgtccg ggtgatcgtt tttacttttc tggtagaggc
     2101 actgtttgcg gtgttcatat atttcaccct ggatgaacga cagttcccct cccactcaga
     2161 aatgatacgg ttttctgttt ttcattctgt ttccgctttt tgtaatgcgg gcttttctct
     2221 gctaagtgat ggtttgtatg atgtgcacta ccggttcaat tatcctttcc tgctgatcat
     2281 cagttttctg gtcattaccg gcggattggg gttcaacatc atattcaact tcttccggta
     2341 tctgaagcac cggctcatca ggctgtcgaa gcaattgttt acccgcaatg tggtgcagta
     2401 caaagcccgc atcatagaag tacacaccaa actcgtcatc atcaccacca cctttctgct
     2461 ggtgttcggg ttcatcatga acctggtgct ggaataccat aatacactgg aagaacacgg
     2521 caccttcgga aaactggtca ccgcattctt tgccagtgtt accccgagaa ctgccggctt
     2581 caataccata gatatgggtg cattgcttcc tgccaccatc ctcatctatt ttctgctgat
     2641 gctgata
//
LOCUS       DAOMAX010000346         2646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8559_length_2646_cov_4.256272, whole genome shotgun sequence.
ACCESSION   DAOMAX010000346 DAOMAX010000000
VERSION     DAOMAX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_2646_cov_4.256272"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PK742_12615"
     CDS             complement(<1..380)
                     /locus_tag="PK742_12615"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HQU77479.1"
                     /translation="MSTNQPKNQKQPNRRFNYYWIYGAIAIFLIGLQFFQFGSSPSKN
                     MTEDRLFGLIEAGDVKKVDIVNDKNVEVYLKDESVKKDKFKEDRIPENGPQYSFNISI
                     GGGFEDRLRQQKTPEHDFDIDNTER"
     gene            complement(389..769)
                     /gene="rsfS"
                     /locus_tag="PK742_12620"
     CDS             complement(389..769)
                     /gene="rsfS"
                     /locus_tag="PK742_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQU77480.1"
                     /translation="MRKRAAKTSLQYSKTDLVINSIQDKKGEDIVVLNLENIPDTVSS
                     RFIICQADSTTQVRAIANHVIDQIGEELGEVPYSKEGLAQCEWVLVDYIDVVVHIFIR
                     DKRQFYQLEDLWHDAAIERIPNFN"
     gene            806..1564
                     /locus_tag="PK742_12625"
     CDS             806..1564
                     /locus_tag="PK742_12625"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQU77481.1"
                     /translation="MRSNHNTVFTGKVLVELDECPSTNDWLKEALRTEKPPEGLVVTT
                     RYQSAGRGQSGRIWQVEPGMNLTFSVLFRPVFLSPAELFLLNQTVALAVYDVVASALP
                     EAQVHIKWPNDLLINGKKAAGILLETTVRKQVETAIIGIGLNVLQTDFPGLPDATSLN
                     REGASMNDPQQLLFPLLQQLETGYLLLRNGRSATGIRQRYHEHLLGMNQKRTFRAADR
                     DFEATVLGVSDDGLLRLEMDGKEERFSVGSIGWE"
     gene            complement(1680..>2646)
                     /gene="metF"
                     /locus_tag="PK742_12630"
     CDS             complement(1680..>2646)
                     /gene="metF"
                     /locus_tag="PK742_12630"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HQU77482.1"
                     /translation="IFAIRMKVTDRIGQSSSTLLSFEVLPPLKGRSVQSIYEVLDPLM
                     AFKPAFVNVTYHRSEYMYKKRSEGIFEKVYTRKRPGTVGICAAIQFKYKVDAVAHLIC
                     GGFTREDTEDALVDLWFLGIENVLLLRGDAARNERHFVAEPGGHPFALDLVKQVVNMN
                     RGQYLEEEHQGGKTDFHIGVAGYPEKHFESPNLKADLKVLKDKVDAGAHYIVTQMFFD
                     NQKFFAFEKSCREAGITVPIIPGLKPITVASQVNMIPRVFHVDIPEALSDAVEKAKSP
                     AEVEKIGAAWCIEQSRELKAAGVPCLHYYTMGKAEVMQNILGEVF"
BASE COUNT          588 a          731 c          664 g          663 t
ORIGIN      
        1 cgttcggtgt tatcgatatc aaaatcgtgt tcaggagttt tttgctggcg cagacggtct
       61 tcaaaaccac cgcctatgct gatgttgaag gaatactgcg gaccgttctc cggaatgcgg
      121 tcctctttga atttgtcttt cttgacgctt tcgtctttga ggtacacctc cacgttcttg
      181 tcgttcacaa tgtccacctt cttcacatcg ccggcctcga tgaggccgaa gagccggtct
      241 tccgtcatgt tcttgctggg tgaactgccg aactggaaga actgcaggcc gatcaggaag
      301 atggcaatgg cgccgtagat ccaatagtaa ttgaatcgcc tgttcggctg tttttgattc
      361 tttggttgat tcgtactcat actgttgttc agttaaaatt ggggatgcgc tctattgccg
      421 catcgtgcca caggtcctcg agctggtaga actggcgttt gtcgcggatg aagatgtgta
      481 cgaccacatc tatgtagtcc accagcaccc attcacactg cgccaggcct tccttgctgt
      541 acggtacctc gcccagttct tcgcctatct ggtctatcac atggttggcg atggcccgta
      601 cctgcgtggt cgaatccgcc tgacaaatga tgaaccggct gctcaccgta tcggggatat
      661 tttccaggtt caggacaaca atgtcctccc ccttcttatc ctgaatacta ttgatgacga
      721 gatcggtctt ggaatattgc agagaggtct tcgcagctct ttttctcaca cccttttact
      781 aatgttacac aaaattacgg attctttgcg cagcaaccat aacacggtct tcaccggaaa
      841 ggttctggtg gaactggatg aatgcccttc caccaacgac tggctgaaag aggccctgcg
      901 cactgaaaag ccgccggaag gtctggtggt caccacccgt tatcaatcgg ccggcagggg
      961 acagtccggg cggatctggc aggtggaacc aggcatgaat ctcacgtttt ccgtcctgtt
     1021 tcgtcctgtt tttcttagcc ccgcagagct ctttctgctc aatcaaacgg tggcgctggc
     1081 ggtatatgat gtggtagctt cagccttacc ggaggctcag gtgcacatca agtggccgaa
     1141 cgacctcctc atcaacggaa aaaaagcggc cggcatcctg ctggagacca ccgtgcgcaa
     1201 acaggtggaa acggccatca tcggcatcgg gctcaatgtg ctgcaaacag attttcccgg
     1261 acttccggat gccacttcac tgaacaggga aggtgcttcc atgaacgatc cgcaacagct
     1321 actctttccc cttctgcaac aactggaaac gggctatctg ctgttgagaa atggccggtc
     1381 agcaactggt atcaggcaac gctaccacga acacctgttg gggatgaatc aaaagagaac
     1441 ttttcgtgcc gccgacagag atttcgaagc gaccgttctc ggtgtatccg atgacggcct
     1501 cctccggctg gaaatggatg gaaaagaaga aagattctct gtaggaagca tcggttggga
     1561 atgaaaaatt cggaagacgg atggcgaaat gctgactggt tagtgtatta cgcagcagtg
     1621 taaattagaa gtcactttgc ggactttgta ttaaaaaaat gaaaattcta tagacttatt
     1681 cagaaaactt cccccaggat attctgcatc acctccgcct tgcccatggt atagtaatgc
     1741 aggcaaggca cccctgccgc tttgagctcg cggctttgtt ctatacacca cgcagcgccg
     1801 atcttctcta cttctgccgg actcttcgct ttctccaccg catcagacag cgcttcgggg
     1861 atgtccacat ggaacaccct cggtatcata ttgacctgcg aggccacggt gatgggcttc
     1921 agtcccggaa tgatgggcac cgtgataccc gcttcgcggc agctcttctc aaaggcgaag
     1981 aacttctggt tgtcgaagaa catctgggtg acgatataat gcgcaccggc atccaccttg
     2041 tctttcagca ccttgagatc cgccttcagg ttgggacttt cgaaatgttt ttccggatat
     2101 cccgccacgc cgatgtggaa gtctgtcttt cccccctggt gctcttcttc caggtattgt
     2161 ccgcggttca tgttcaccac ctgcttcacc agatccagtg cgaatggatg tccgcccggc
     2221 tctgccacaa aatggcgttc gttacgggcc gcatcaccac gaagcaacag cacgttctct
     2281 atgcccagga accagaggtc caccagggca tcttccgtat cctcacgggt gaaaccgccg
     2341 cagatcaggt gcgccacggc atccaccttg tacttgaact ggatggccgc acagatgccc
     2401 accgtacccg gacgcttgcg ggtataaact ttttcaaaga tgccctcgct gcgcttcttg
     2461 tacatgtact cactgcggtg gtaggtcacg ttgacaaatg ccggcttgaa cgccatgagc
     2521 ggatcaagca cctcatagat gctctgcaca ctgcgcccct tgaggggagg cagcacctca
     2581 aaactcagca gcgtactgct gctctgtcca atgcgatcgg tcaccttcat gcggatggca
     2641 aatata
//
LOCUS       DAOMAX010000347         2631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8633_length_2631_cov_3.982919, whole genome shotgun sequence.
ACCESSION   DAOMAX010000347 DAOMAX010000000
VERSION     DAOMAX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8633_length_2631_cov_3.982919"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..494
                     /locus_tag="PK742_12635"
     CDS             147..494
                     /locus_tag="PK742_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQU77483.1"
                     /translation="MEKPSAARDIILSLKERFRPEKADPGYEAVFHLDISGDRGGQYT
                     VSINDGTITVEEGHSSEATCTISASDDTYEDVEWGRTNPQMAFLFGKVKVSDIGAMLD
                     FAGLFHRCEDFYK"
     gene            498..2153
                     /locus_tag="PK742_12640"
     CDS             498..2153
                     /locus_tag="PK742_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77484.1"
                     /translation="MKRILFALAGILALSGVEAQVASNFNGLSVITGNLYPTENNKGI
                     RIQNEMIQLRELDTSSIYDILYDCKNTGTQYGVVNVTQPIHLYFNEFRPGYRPEMLDQ
                     MSKMFIDLFQVADPNMDIRDQLKDNFGQRLFVRRYVSSDNLKTLGIYADLFKGSTRLN
                     YNKVWIEFKWIDDDPYNLEKNTETLSMEIRMSTDIQFAPNEQFNLLSFMKLPTTLCGI
                     KQKQYYSPYQLGFENKWAGVIDKLYLQHDIFNATPIIPSGIRYQTMYSGERDQVLVLN
                     NVDVQNDQGIAFFAIRENLQPCGNTRLVEEKSIIPAPVTNVTASSWDKAPVRLDNRNY
                     VVTYETSLTDTLQAYQTGNPTDLDLFSKGLQRKMYSSYLLNDYQTGNCKGEKPGIEMK
                     EGGHPIFAFDISNFSQEDSAFRNKKNLALQTCWCEGAQGNGKGEYIEFTLTQPSNAVS
                     IFNGNQRSNETFDGHTMADVIRFHSPDGLIEDKKYSIIDLSIKNLYPVYMPAGTYRIY
                     LDDVSSKGTPTTCLSSILFDFVLEDDWYQKGVKMLEGAYKTNK"
     gene            2150..>2631
                     /locus_tag="PK742_12645"
     CDS             2150..>2631
                     /locus_tag="PK742_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HQU77485.1"
                     /translation="MTDVRKAAAILLARTGLPDQLLDTLPVGGGCINDCYQLRTDQDT
                     FFLKVNDAKAFPKMFKAEARGLTLLADHFQGAIPQVVYEEVFGDQQYLLLTWIEQGCA
                     GPNGMALLGEFLARMHRVSAPVFGLDTDNYMGSLPQQNTPTESFADFYRDSRLMPLYK
                     "
BASE COUNT          715 a          697 c          633 g          586 t
ORIGIN      
        1 ggagctgacc gcctctgctg ccacacttct ggtcttgaag aggcgtacgg cccacagcca
       61 tttatcgatc cggcatttgt ctggagtttc cacagccgta aaattaaact cttgccccga
      121 acgtttattt ttacgcaaaa agcagcatgg agaagccgag cgcagcccga gacatcatcc
      181 tttcactcaa agaacgcttc cgtcctgaaa aggcagaccc cggttatgaa gctgtgtttc
      241 acctggacat ctccggcgac cgtggcggac aatacaccgt ttccatcaac gatggcacga
      301 ttactgtgga agagggacac agcagcgagg ctacctgcac catcagcgcc agcgacgata
      361 cctacgaaga tgtggaatgg ggacgcacca atccgcagat ggccttcctc ttcggcaagg
      421 tcaaggtgag tgatatcggc gctatgctgg attttgcagg actgttccat cgctgtgaag
      481 acttttacaa atagactatg aaaagaatcc tattcgccct ggccggcatc ttagcccttt
      541 ccggtgtgga ggcacaggtt gccagcaact tcaacggcct gtcggtcatc accggcaacc
      601 tctaccccac cgagaacaac aaaggcatcc gtatccagaa cgagatgatc cagctgagag
      661 agctggacac ttcgtccatc tacgacatcc tgtatgactg caaaaacacc ggcacgcaat
      721 acggtgtggt caacgtgacc cagcccatcc atctttactt caatgagttt cgtccgggat
      781 atcgccccga gatgctggac cagatgagca aaatgttcat cgacctcttc caggtggccg
      841 atccgaatat ggacatccgc gatcagttga aagacaattt tggtcagcgc ctgttcgtgc
      901 gtcgttatgt gagcagcgat aacctcaaga ccctgggcat ctacgccgac ctgttcaagg
      961 gcagcaccag gctgaactat aacaaggtct ggatagaatt caaatggata gatgacgatc
     1021 cctataacct ggagaagaac accgaaacgc taagtatgga gatccgcatg agtacggata
     1081 ttcagttcgc ccctaacgaa cagttcaatc tgctcagttt tatgaaactc cctaccaccc
     1141 tgtgtggtat caaacaaaag caatactatt caccctatca gcttggtttt gaaaacaagt
     1201 gggcaggagt catagacaag ctgtatctgc agcatgacat tttcaatgct acgcccatca
     1261 tccccagtgg catccgttac cagacgatgt acagcggaga acgtgatcag gtgcttgtac
     1321 ttaataatgt ggacgttcaa aatgatcagg gcatcgcttt ttttgccatt cgcgaaaacc
     1381 tgcagccttg cggaaacacc cgccttgtgg aagaaaagtc catcatcccc gctcctgtta
     1441 ccaatgtaac tgccagcagc tgggataaag ctccggtgcg tctggataac cgtaattatg
     1501 tggttactta tgaaacaagt ctgaccgata cgctgcaagc ctatcagacc ggcaacccga
     1561 cagacctcga tctgttcagt aaagggctgc aacggaaaat gtacagttcc tatctgttga
     1621 atgactacca gaccggaaat tgcaaaggag aaaagccagg catcgaaatg aaagaaggag
     1681 gtcacccgat cttcgctttt gatatcagca atttcagcca ggaagactct gctttcagaa
     1741 acaaaaagaa cctggcactg caaacctgct ggtgcgaagg agcgcagggt aatggcaagg
     1801 gcgaatacat cgagttcacc ctcacccaac ccagcaatgc agtgagcatt ttcaatggca
     1861 accagcgcag caatgaaacc ttcgatggtc atactatggc cgatgtgata cgtttccatt
     1921 cgcccgacgg attgatagaa gacaagaagt attccatcat cgacctgagc atcaagaacc
     1981 tgtatcctgt ttatatgccg gcaggcacct accgcatata cctggacgat gtttcatcca
     2041 agggaacccc caccacctgt ttgtcatcca tcctgtttga tttcgttctg gaagatgact
     2101 ggtaccagaa aggcgtgaag atgctggaag gagcctacaa gaccaacaag tgaccgatgt
     2161 ccggaaggca gcagcaatcc tccttgcacg gacagggctg cccgatcagt tgctggatac
     2221 acttcctgtg ggcggaggct gcatcaatga ctgctatcag ctaagaaccg atcaggatac
     2281 cttcttcctg aaggtcaatg atgcgaaagc atttccaaaa atgttcaaag cagaagcccg
     2341 cggactgaca ttacttgcag atcattttca gggtgcgata ccgcaggtgg tgtatgagga
     2401 agttttcgga gatcagcaat acctcctgct tacctggata gaacagggct gtgccggccc
     2461 gaacggaatg gcccttctgg gagaatttct cgccaggatg caccgggttt ccgctcctgt
     2521 tttcggactg gatacagaca actacatggg ctcgttgcca caacaaaata cgccgacaga
     2581 aagctttgca gacttctacc gcgacagccg cctgatgccg ctttacaaac a
//
LOCUS       DAOMAX010000348         2620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8699_length_2620_cov_4.336842, whole genome shotgun sequence.
ACCESSION   DAOMAX010000348 DAOMAX010000000
VERSION     DAOMAX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8699_length_2620_cov_4.336842"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /gene="rseP"
                     /locus_tag="PK742_12650"
     CDS             complement(<1..960)
                     /gene="rseP"
                     /locus_tag="PK742_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQU77486.1"
                     /translation="MVIIQVAQLILSLTILVFIHELGHFLAARMFGVRVEKFFIFFDA
                     WGKKLFSFKRGDTEYGIGWLPLGGYVKIVGMIDESMDKEQMKGEPQPYELRSKPGWQK
                     FIVMIAGIVMNVILGIIIYTIIHNHFDKSYYSVGTINQDGIYASAGARDMGFQTGDKL
                     VAVNGKHFDRYDDYVSMKVLFGAKVTVERNGKDVVIDVPNDYFQQVRAGYGFIEPLST
                     VVIDEFPDTSSARDAGLQVGDQLIAVDGQPISCYGEFKEKMAASKGGQVAVTVFRGKD
                     TVTIDAPVDTAGALGFVAQIKLLDKYQTTDYKLGSAFVYGARDA"
     gene            complement(1019..2464)
                     /locus_tag="PK742_12655"
     CDS             complement(1019..2464)
                     /locus_tag="PK742_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQU77487.1"
                     /translation="MYAEQKNAVWHQEQILKDLCFRAGLTVFGQEHDLMGVKSHADLV
                     KRVPVRDYEGLRPWFDRVKNGEPDILWPGKPLYLAKTSGTTSGAKYIPITQASMPNHI
                     TSARNALLSYIHETGKTGFIAGKMIFLQGSPVLQEMHGIQMGRLSGLVYHHVPNYLLR
                     NRLPSYEINCIEDWEEKVEAIARETIKENMSLISGIPPWVIMYFEKLKELSGKETVKD
                     IFPNFSLFVYGGVNYEPYRHKMEELMGKRIDSVETYPASEGFIAFQDSQKEPGLLLNV
                     NSGIYFEFIPVSEYHNEHPKRLSLAEVDTETNYALVLHTNAGLWGYSIGDTVRFVSLN
                     PYRIVVTGRIRHFISAFGEHVIGEEVEYAMQQAAAVAGVRVSEFTVAPQVDPAEGLPY
                     HEWLVEFDVPPSDMAHFAADLDRHLQEKNIYYKDLITGAVLRPLVITPIENGGFREYM
                     KSQGKLGGQNKVPRLSNDRKIADELIRWRSR"
BASE COUNT          616 a          673 c          659 g          672 t
ORIGIN      
        1 ggcatcgcgg gctccgtaaa caaatgcact gccgagtttg taatcggtgg tttgatattt
       61 atccagcaat ttgatctgtg ctacaaaacc aagtgcccct gcagtatcaa ccggggcatc
      121 tatggtgacg gtatcttttc cgcgaaatac agttaccgca acctgaccgc ctttggaagc
      181 agccatcttt tccttgaatt ccccatagca tgaaattggc tggccatcaa cagcgatcaa
      241 ttggtctcct acctgaagtc ctgcatctct ggcagaagaa gtatccggaa actcatcaat
      301 cacgacagta ctgagtggtt caataaagcc atagcctgca cgtacctgct ggaaataatc
      361 gttgggcacg tcgatgacca catctttgcc attgcgctct acggtcacct ttgccccgaa
      421 cagcaccttc atagaaacgt agtcatcgta gcggtcgaaa tgttttccgt tcaccgccac
      481 gagtttatcg cccgtctgga atcccatatc gcgggcaccg gctgaagcat agataccatc
      541 ctgattgatg gtacctacgg aataatagct cttatcgaaa tgattgtgga tgatggtata
      601 gatgatgata cccaggatca cgttcatgac gataccggct atcatgacga tgaatttctg
      661 ccagcccggt ttggaacgca gttcatatgg ctggggttcg cccttcatct gctccttgtc
      721 catgctctcg tctatcatgc cgacgatctt cacataacca cccagaggca gccagccgat
      781 gccgtattcg gtatcgcctc tcttgaagga aaagagcttt ttgccccagg catcaaagaa
      841 tatgaagaac ttctccaccc ggaccccgaa catgcgggca gccagaaagt ggccgagttc
      901 gtgaatgaac accaggatgg tgagggataa tatcagctga gctacttgta taatgaccat
      961 aggagtgcaa aattcgtcaa ataacgccgg atggcggatg gtattggttt aaatcttgtt
     1021 aacggcttct ccagcgaatg agttcatcag cgatcttccg gtcgttggag aggcgtggca
     1081 ccttgttttg tccgcccagt ttcccctgtg acttcatgta ttctcggaat ccgccatttt
     1141 ctataggtgt gatgaccagg ggccggagca cagcacctgt gatgagatcc ttataataga
     1201 tgttcttctc ctggaggtga cgatccaaat cggcagcaaa atgtgccata tcagacggag
     1261 gtacatcaaa ttccaccagc cattcgtggt agggaaggcc ttctgcggga tcgacctgcg
     1321 gggcaacagt gaattcgctg acgcggacac ctgctacggc agctgcctgt tgcatggcgt
     1381 actccacttc ttcaccgatg acatgttcgc cgaaagcgga gatgaaatgc ctgatgcgtc
     1441 ctgttaccac gatgcggtaa ggattgaggg agacaaagcg cacagtatcg ccgatgctgt
     1501 atccccacag accggcattg gtatgcagca cgagtgcata gtttgtttct gtgtccacct
     1561 ctgccagcga caatctcttc ggatgttcgt tgtggtattc gcttaccgga atgaattcaa
     1621 aatagatgcc gctgttcacg ttgagcagca gacccggttc tttctgactg tcctgaaagg
     1681 cgatgaatcc ctccgatgcc ggataggtct caacgctgtc tatgcgtttg cccatcagct
     1741 cctccatctt gtggcggtag ggttcgtagt tgacaccacc atagacgaaa agtgaaaaat
     1801 tcgggaagat gtctttgacc gtttccttac cgctgagctc tttgagtttt tcgaaataca
     1861 tgatgaccca tggcgggatg ccggagatga ggctcatgtt ctctttgatc gtttcacggg
     1921 cgatggcttc taccttttct tcccagtctt ctatgcaatt gatctcataa gaagggaggc
     1981 gattgcgcag caggtaattg ggcacatggt gatagaccag acctgataac ctgcccatct
     2041 gaatgccatg catctcctgc aacaccggac tgccctgcag gaagatcatc ttacctgcga
     2101 tgaagccggt ctttcccgtc tcgtggatat aactcagcaa ggcattcctg gccgaagtga
     2161 tgtggttggg catggacgcc tgggtaattg gaatatactt ggctccagac gtggtgccgg
     2221 aggtcttggc gaggtagagt ggttttcccg gccagaggat atcgggttca ccattcttca
     2281 cccggtcgaa ccagggcctg aggccttcgt agtcacgcac cggcacacgt ttcaccagat
     2341 ctgcatgaga ctttactccc atcagatcat gttcctgccc gaacacggtc agccctgccc
     2401 ggaaacagag gtccttcaat atctgttcct gatgccagac ggcattcttc tgctccgcat
     2461 acatccttcg aaccaccagg gcggcaaagg gtctggaaag gaggcctttg aaattcacgt
     2521 tgcaaagata gaataccgct gtggacgatt gaatagttag tatgtggatc gcttttcgtc
     2581 gttcgatacg ggcaaatgga aatgtgagat cattttcctc
//
LOCUS       DAOMAX010000349         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8736_length_2613_cov_3.767787, whole genome shotgun sequence.
ACCESSION   DAOMAX010000349 DAOMAX010000000
VERSION     DAOMAX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8736_length_2613_cov_3.767787"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PK742_12660"
     CDS             <1..748
                     /locus_tag="PK742_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77488.1"
                     /translation="HYLQNGTSFASPAVTGIAALYLQKNPAADWSEVNDAILSNARRD
                     SWTGDALPDNNWGYGKADAFRTLTGSWGCDSSNYMDPPAGLTAADITPISVALEWSLI
                     PNASGYQIAYAPVAGGSVLRKKSPVNSKTIGGLLPGTTYSCKVRAACPDFGFSSWSIP
                     FVFTTDPLREGVVNQAVIYPNPSSGTLHISYPGEKFRFRIMDLQGHILYSDQATEAAR
                     ADLSVLPSGIYLVEFTDSSGSSSRQCLILE"
     gene            748..1263
                     /locus_tag="PK742_12665"
     CDS             748..1263
                     /locus_tag="PK742_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQU77489.1"
                     /translation="MEATLTRIADWPQVQQKIKRIAFEIAEDNYDEPEIIVAGICRNT
                     EGYAMAVKIYDVLKEIVQTKVVLTHIELDKSRPADSPVEMSLSDSDINGKVIILVDDV
                     SNSGRTLLYAMKPFLEHHPKKIQMAVLVERTHKMFPVQPDYVGLSLSTTIQEHITVVI
                     SRAGEEGIYLS"
     gene            complement(1251..1769)
                     /locus_tag="PK742_12670"
     CDS             complement(1251..1769)
                     /locus_tag="PK742_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HQU77490.1"
                     /translation="MRIYVLGYMGSGKTTQGQKLAKKLGLDFYDLDALIEDAAGMKVA
                     EIFAEKGEQHFRELEADVLRGTVLFPDAVIACGGGTPCYYNNMQWMNNRGATVWFHLP
                     PEIIAGRIKKKKAKRPLIAHLPDEEILPFIREKLQERSYFYGKAQFHFDLSEMSVKEI
                     AAGLCEAIPQLR"
     gene            complement(1766..>2613)
                     /locus_tag="PK742_12675"
     CDS             complement(1766..>2613)
                     /locus_tag="PK742_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77491.1"
                     /translation="PAARNYLEPGSVKAITIMSSSSHRWVLKYPYLGRSFQSGRPVAS
                     LLNATIPNTLLLALTAMMFATFCGIILGMLAAMQQGSWLDRSITAGSVMGIALPSFFA
                     AILLQLIFAYLLSSFTGLKMTGNLYEADEYTGQIYLSLRNLILPAIALGIRPVAVITQ
                     ITRSSMLDVLHADYIRTARAKGLGSGAVVYRHALKNALIPVITSVTGWLASLLAGAFF
                     IEVVFNYNGLGLETVNAIKTKDIPVATGAVLYIALVFVLVNMLTDILYGLADPRVKMS
                     SKQAS"
BASE COUNT          687 a          661 c          649 g          616 t
ORIGIN      
        1 gcactatctg caaaacggaa cctcgttcgc atcacctgca gtaacaggaa ttgcagcgtt
       61 gtatctgcaa aaaaatcctg ctgccgactg gagtgaggtc aacgatgcca tactcagcaa
      121 tgcacgtcgc gacagctgga caggagatgc cttgccagac aataactggg gttatggaaa
      181 ggccgatgcc ttcagaacgc tgacaggcag ctggggttgt gacagcagca attacatgga
      241 tcctccggcc ggactaacag ctgcagacat caccccgatc tctgtggctc tggaatggag
      301 cctgataccc aatgcttcgg gttaccagat cgcctatgca cctgtagcag gaggaagtgt
      361 gcttcgcaag aaatcgcccg taaacagcaa gaccattggc ggcttgttac ccggtactac
      421 ttatagctgc aaggtacgtg cagcctgtcc cgacttcggt ttttcgtcct ggagtatccc
      481 atttgttttc actacagacc cgcttcgcga gggtgttgtg aaccaggctg tgatctatcc
      541 aaacccaagc agcggaacat tgcatatttc ttacccggga gaaaaattcc gtttccgtat
      601 catggatctg caagggcata tcctgtacag tgatcaggca actgaagctg cccgggcaga
      661 tctgtcagtt ttgccatcag gtatctatct ggtagaattc acagacagtt caggcagttc
      721 ctcccgtcaa tgtctaattt tggagtgatg gaagcgacac ttacccgaat agcagactgg
      781 ccgcaggtgc agcagaagat caagcgcatt gcatttgaaa tagccgaaga caactacgac
      841 gaacccgaga tcattgtagc cggtatttgc cgcaacacag aagggtatgc gatggcggtc
      901 aagatctatg atgtactgaa agagatcgta caaacaaaag tggtgctgac acatattgaa
      961 ctggacaaaa gcagaccggc agacagtccg gttgagatga gtctgtcaga cagcgacatc
     1021 aacggcaagg tcatcatcct ggtggacgat gtatccaaca gtggccgcac cctgctgtat
     1081 gccatgaagc catttctgga acaccatccc aaaaagatcc agatggcggt actggtggag
     1141 cgtacccata agatgtttcc ggtgcagccg gattatgtgg gattgtcttt aagcaccacg
     1201 attcaggaac acatcacagt ggtcatcagc agagcaggtg aagaaggtat ttacctcagc
     1261 tgaggaatag cctcacataa cccggcagcg atctctttta cggacatttc agacaggtcg
     1321 aagtgaaatt gtgccttccc atagaaatac gatcgctcct gtaatttctc tctgatgaac
     1381 ggaagtatct cttcatcagg cagatgagca atgagtggac gctttgcttt tttctttttg
     1441 atacgtccgg cgatgatctc aggaggcaga tgaaaccaga ccgttgcacc gcggttattc
     1501 atccactgca tattgttgta atagcaagga gtgccgccac cacaggctat gaccgcatca
     1561 ggaaacaaaa cggtaccccg caatacatct gcttccagct ctctgaaatg ctgttcccct
     1621 ttttcggcaa agatctcggc caccttcatc cctgctgcat cttctatcag ggcatccaga
     1681 tcataaaaat caagtccgag ttttttggcc agtttctgcc cctgggtcgt cttgccgctg
     1741 cccatatatc ctaacacata aattctcatg atgcttgttt gctgctcatt ttcacccgtg
     1801 ggtctgcaag cccgtacagg atgtcggtca gcatattgac caacacaaaa accagtgcaa
     1861 tatacaatac ggcacctgtt gctacaggaa tatctttcgt cttgatggca ttgaccgttt
     1921 ccagtccgag gccgttataa ttgaacacca cttcaataaa aaatgcaccg gccaacagag
     1981 aagccagcca tcccgtaacg gaagtaatga ccggaatgag tgcatttttc agtgcgtgtc
     2041 tgtacaccac cgcacctgac ccaagtcctt ttgcccgggc agtgcggatg taatctgcat
     2101 gtaacacatc cagcatgctg cttctggtga tctgggtgat gactgcaaca ggcctgattc
     2161 ccagggcgat ggcaggcagg atgagattgc gaagtgacag gtagatctgg ccggtatatt
     2221 catctgcttc atacagattg cccgtcatct tcagacccgt gaaactgctc agcaaatagg
     2281 cgaaaatgag ttgaagtaag atggctgcaa aaaaggaagg cagggctatt cccatgaccg
     2341 atcctgcggt gatgcttctg tccagccagg aaccctgttg catggccgcc agcattccga
     2401 gaatgatgcc acaaaaagtc gcgaacatca tggccgtcag tgccagcagc agggtattgg
     2461 ggatggtggc attgagcagc gaagccaccg gtctgccaga ctgaaaggaa cgacccagat
     2521 agggatattt caatacccag cgatggcttg aactactcat aatagtaatg gctttaacag
     2581 accccggttc caggtagttt cttgctgcag ggt
//
LOCUS       DAOMAX010000350         2586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8875_length_2586_cov_4.119716, whole genome shotgun sequence.
ACCESSION   DAOMAX010000350 DAOMAX010000000
VERSION     DAOMAX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8875_length_2586_cov_4.119716"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1252
                     /locus_tag="PK742_12680"
     CDS             326..1252
                     /locus_tag="PK742_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77492.1"
                     /translation="MPVRVTKLLLITLMSWSFGPTNGQSIKALPVSFSANLIYLNIPL
                     PDNDTLVMYLDTGGKNFMYKSGLKKLEIRPTRKNLHSRVRLDEIFLQNSIPVSYKKDL
                     YFLNDKDNTCDGMLGREWYANKIWLIDYRNQQMFAIESYSEEILSISATIPVSFRKDT
                     SGSISNHLPGVQVVIDGDTLSMLLDTGAQAQLSDQATSAFNQSGTSSISFIDQSTFEK
                     WEQLHPDWRVVKGGDTSLRVAEDIIEVPGVNIGDTVVGPVYFAKRADQNFVVMSNNFM
                     DQQITGALGANALSQLGQIILDYPQQRLLVLE"
     gene            1312..>2586
                     /locus_tag="PK742_12685"
     CDS             1312..>2586
                     /locus_tag="PK742_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77493.1"
                     /translation="MKKYLLAALMSPLISHGQSIGISVYDLISDAPLPYANIYFIHSG
                     IGSSANQYGQTAFLSSDLLTTDSFVVSYIGYDKQTFAFSKATYKEPYIIKLAPSQHLL
                     SEIVIHSENQQDPKKIIKQAIKNTTANYTSDPTLYYTLYRETVAENNRFIQLNEAILL
                     THYTGYPQKKLDPDLWKAYYYDDAYAFYLDGNRFFHPLLNDLNTANDKQSIIATRHSD
                     NWSDHGIETTLIGDPLLLFALDKIKYQYDFFNPSILHKYQFNNEPDEYIDSAECYVIS
                     FYPKETEKHFVIDQSKKNRSAIYAGRLYITKNTFALVGFEYVLVAERDFGFFNSSMPL
                     EYRVKMEYQESNGKYHIRYICQTETRKVDTNQKGESILHNAKREIYVLDTATIHVQDP
                     DSLARFKTTRYSSVRHFPDNYDPDYWKNLPNEE"
BASE COUNT          833 a          594 c          452 g          707 t
ORIGIN      
        1 gatggtattc agccgtatga agtccatgag gaagcccttg gagtgaacat ctttgtttct
       61 tcttattctg atatcagatc aattttgaac aattgattat ctattcccat catattcttc
      121 cgaaaagctc catattctgc agcaaatcat ccgttttcaa tgccacgctg taatctccat
      181 acaatagctg tagctgaaat catgaatcgg ctgcaatcaa cagccataca ttcttttact
      241 attacatgcc tcaaatccat acagaaatac cataaaaaag agcaggcaat aacatattga
      301 aaaataaatc gttaaaccac ctatcatgcc tgtacgtgta acaaaactgc ttcttattac
      361 cctgatgtcc tggtcattcg gacctaccaa tggccagagt ataaaagcac ttccggtatc
      421 gttctcagcc aaccttatct atctgaatat tccgctaccc gataacgaca cactggtcat
      481 gtaccttgat accggcggta agaattttat gtacaaatca ggactaaaaa aattagaaat
      541 aagacctaca cgaaagaacc tgcattccag ggtccgtttg gatgaaattt tcctgcagaa
      601 ttcaatacct gtatcgtaca aaaaagatct ctatttcctg aacgataaag acaacacgtg
      661 cgatggcatg cttggcagag aatggtacgc caacaaaatc tggctcattg actatcgtaa
      721 tcagcagatg tttgccattg aatcatattc ggaggaaata ctgtctatat ctgccactat
      781 tcctgtatct tttcgaaaag acacttcagg aagcatctcc aatcacctcc ccggagtaca
      841 ggtggttata gatggagata ccctgtctat gcttcttgat accggagcac aggcacaatt
      901 aagtgaccag gcaaccagtg ctttcaatca gtcaggaacc agttccatta gtttcattga
      961 tcagtcaaca tttgaaaaat gggaacaact tcatccggat tggcgggtgg taaaaggtgg
     1021 agatacctct ctcagagtgg ccgaagatat cattgaagta cccggtgtca acattggtga
     1081 tacagtagta ggcccggtgt attttgccaa aagagctgat caaaatttcg tggtcatgtc
     1141 aaacaatttt atggatcaac agataaccgg tgcgttagga gccaacgccc tgtcgcagct
     1201 tggtcagatc attctggatt atccgcaaca gcgattgctt gtattagaat gaactgaaca
     1261 gaacacaaag cttctttagc aacttaaaca acacatcact tacaagcacc aatgaagaag
     1321 tacttgctgg cagcactgat gtcaccactg atctcacacg gacagtctat tggcatatcc
     1381 gtctatgacc tgatctcaga cgcccccctt ccttacgcca atatttactt tatacattca
     1441 ggcatcggtt catcagctaa ccagtacgga caaactgctt tcttatcatc agatctgcta
     1501 actacagact cctttgtcgt atcatatatt ggttatgata agcagacctt tgccttctcc
     1561 aaagcaactt acaaagaacc ctatataata aagcttgcac cttctcagca tctcttgtct
     1621 gagattgtaa tccattctga aaatcaacag gatccgaaga aaatcattaa gcaagctata
     1681 aaaaacacca cagccaatta caccagtgac cctactctgt actatacact ctatcgtgaa
     1741 acagttgctg aaaacaatcg ctttattcag ttgaatgaag ccattctcct tacacactat
     1801 acgggatacc cacaaaagaa actggaccct gatctatgga aagcctatta ctatgatgac
     1861 gcctatgcct tttatctgga tggtaatcgc ttttttcatc ccctcctaaa tgatcttaat
     1921 acagcaaacg acaaacaaag tattattgct acaaggcatt cagacaactg gagtgatcat
     1981 ggtatagaaa caaccctaat tggagaccca ttgttgctgt ttgcccttga taagatcaaa
     2041 taccagtatg atttcttcaa cccatccatt cttcataagt atcagttcaa caatgaacct
     2101 gatgagtaca ttgattctgc agagtgctat gtcatttctt tttatccaaa agaaacagaa
     2161 aaacactttg tcatagacca gagtaaaaaa aacagatctg ccatatacgc cggacggctg
     2221 tatataacca aaaatacatt cgcacttgtt ggttttgaat atgttcttgt tgcagaaaga
     2281 gactttggat tcttcaacag ttccatgcct ctggagtatc gtgtaaaaat ggagtaccag
     2341 gaaagcaatg gaaaatacca tatccgctat atctgtcaaa cagaaaccag gaaggtagat
     2401 acaaaccaaa agggcgaatc aattcttcac aacgcaaaaa gagaaattta tgtgcttgac
     2461 acagcaacca ttcatgttca agatcccgac agtttagccc ggttcaaaac aacccgatat
     2521 tcttcagtac ggcattttcc agacaactac gatcctgact attggaaaaa ccttcccaat
     2581 gaagaa
//
LOCUS       DAOMAX010000351         2580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8915_length_2580_cov_3.723960, whole genome shotgun sequence.
ACCESSION   DAOMAX010000351 DAOMAX010000000
VERSION     DAOMAX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8915_length_2580_cov_3.723960"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..990
                     /locus_tag="PK742_12690"
     CDS             382..990
                     /locus_tag="PK742_12690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU77494.1"
                     /translation="MRQPNNFNFVAPFYRWLSLMVYGRKLEAVQHTHLNLIEPGANIL
                     VLGGGSGEILNHLPADSHITFLDLSEAMLDRAKKKRRVHFVHADFLQWESTEHFDCII
                     TPFFLDCFAPDDLQTAIQKIKVLLQPDGRLIVVDFYHDGRRTTGFLLRLMHLFFRWTA
                     NLSSGKLQDIHATVLQEGFFCIEKQEERGSTFSAVYIRSGDE"
     gene            complement(1092..1865)
                     /locus_tag="PK742_12695"
     CDS             complement(1092..1865)
                     /locus_tag="PK742_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HQU77495.1"
                     /translation="MNKIHIMYLAISILLLSGCAIVRPGEAGIKTTLGKVDDKVLVQG
                     PKLYFPFTSRVIKLPVRTENIEIAVDLPSKEGLTIRSDISILYRLETESVVKVYQDVG
                     LNYEQTIILPIFRSAASDVSAQFFAKDMHSGERASIERRIQTLMTERLEGRGIIIESV
                     LMKSIKLPDGLSRAIEDKLEAEQRAQQMQFILDRERQEAQRKKVEAEGVRDAQMIITE
                     GLNPMIIQFMSIEAFEKLAESDNTKVIITDGKSPFLIEQ"
     gene            1985..2110
                     /locus_tag="PK742_12700"
     CDS             1985..2110
                     /locus_tag="PK742_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77496.1"
                     /translation="MRTSFRVGIPIFILFQSATMSSAQELQTVPYVDLERYAGLW"
     gene            2114..2512
                     /locus_tag="PK742_12705"
     CDS             2114..2512
                     /locus_tag="PK742_12705"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQU77497.1"
                     /translation="MASYPQRFQEGCTATTAEYTLSDKGYVIVENRCNKYTLDGKQSY
                     IKGKAFVVKNSGNAKLKVQFFWPFRGSYWIIDLADDYSYAVVSHPGKKYLWILSRTPQ
                     MDPATYDGILERVRAQGLDTDKLKVTLQAE"
BASE COUNT          676 a          623 c          614 g          667 t
ORIGIN      
        1 tgcaacgatg gccacaagga tctgtgaaag tgggtgcata atggatcagg ttggtgttaa
       61 atgtaagggt tttggtggga aggcagattg atcaaagatc acccctcctc ctgcgggtat
      121 tccccgtccg gggggacagt ttcccccaaa tcctttgatc aattgatcgt ggttctgtgt
      181 tctcggaggt acaaacaatt ataaatgaca agcgaagaag cagcacaaag gaacaattta
      241 ctaaaatgaa tcaaaggata attgtccccc ctcgcagggg ggtgaagggg ggtgatgtac
      301 atgcactcaa catttgttta ttgaaacata aaccttctgg tttgacaaac aactaaaata
      361 ccccttactt cgtaagaccc catgcgccaa cccaacaact tcaactttgt ggcaccattc
      421 taccgatggc ttagccttat ggtgtacggg cgaaaacttg aagctgtaca gcacacacat
      481 ctgaacctga tagaacctgg tgcaaacata ttggtgttgg gaggaggctc cggagagata
      541 ctgaaccacc ttcctgctga cagccacatc accttcctgg atctgtcaga agccatgctg
      601 gatcgggcga agaaaaagcg acgggtgcac tttgttcatg ccgactttct gcaatgggaa
      661 tcaaccgaac atttcgactg catcatcacg cctttcttcc tggattgttt tgcaccggat
      721 gatctgcaga cagccatcca gaaaataaaa gtcctgctgc agccggatgg caggttgatc
      781 gtagttgatt tctatcatga cggccgacgc accaccggat tcctgctccg cctcatgcac
      841 ctctttttcc ggtggacggc caacttatca tccggaaaac tacaggatat ccacgcaact
      901 gtattgcagg aaggattttt ctgtatcgaa aaacaggaag aaaggggaag caccttcagt
      961 gcggtgtata tcagatccgg agatgaatag atccggagac tttcctctaa tgctgaaaaa
     1021 aaaatcccgg ctgcagggac acaaccgggg taatggattt taaagctcgg tcacaccagg
     1081 ctgtccggac tttattgttc aattagaaac ggactctttc catctgtgat gatcactttt
     1141 gtattatcag actccgccag tttttcaaat gcttcgatcg acatgaactg aatgatcatc
     1201 ggattaagcc cttcggtgat gatcatctgc gcatcccgca caccctctgc ttctactttc
     1261 ttccgctgag cttcctgtct ttccctgtcc aggatgaact gcatctgctg ggctctttgt
     1321 tcggcttcca gtttgtcttc gatggcacgg gaaagtccat caggtaattt gatgcttttc
     1381 atcagcacag actcaatgat gatgcctctg ccttccaggc gttcggtcat cagcgtctgg
     1441 atccggcgct ctatggatgc acgctcgccg gaatgcatgt ctttggcaaa gaattgtgca
     1501 ctcacatctg atgctgctga tctgaatata ggcaggatga tggtctgctc gtagttcagt
     1561 cccacatcct gatagacctt taccaccgac tcagtctcca gtctgtacag aatggaaata
     1621 tcagacctga tggtaagtcc ttccttggaa ggaagatcca ctgcgatctc aatgttctca
     1681 gtgcgtaccg gtaatttaat gaccctggaa gtgaagggga aataaagctt cggtccctgt
     1741 acaagtactt tgtcatccac cttccccagg gtggtcttga tgccggcttc acccggacga
     1801 acgatggcac aaccagagag cagcaggatg ctgatcgcca ggtacatgat gtgtatttta
     1861 ttcataaggc ttttatttta atagagacag tttacacaaa tcaattgttc acggaacaga
     1921 cataaagtgg agactgattt gcaactattg tccggagcaa tttgttgtac tacacaacag
     1981 ccatatgaga accagttttc gggtcggtat tccgatcttc attctgtttc aatctgcaac
     2041 catgtcttca gcacaggaac ttcagaccgt accctatgtg gacctcgaac gctatgcggg
     2101 tctgtggtaa gagatagcct cttatccaca aagattccag gagggctgta cggctaccac
     2161 tgcagaatac acgctgtctg acaaaggata tgtgatcgta gagaacaggt gcaacaaata
     2221 caccctggat ggcaaacagt cgtacatcaa aggcaaggct ttcgtggtaa agaactcagg
     2281 caatgccaaa ctgaaggtgc agttcttctg gccgttcaga ggcagctact ggatcattga
     2341 cctggcggat gattacagct atgccgtggt ttcgcatccg gggaaaaaat acctgtggat
     2401 actatcccgc accccgcaaa tggatcctgc aacctacgat ggcattttgg aacgtgtccg
     2461 tgcccaggga ctggataccg ataaactgaa ggtgacactg caggctgaat aagcttctga
     2521 caggttgcag catgctattg tgaaatgatc acctgccatc cggataggtg ctttgcatat
//
LOCUS       DAOMAX010000352         2573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_8944_length_2573_cov_7.399921, whole genome shotgun sequence.
ACCESSION   DAOMAX010000352 DAOMAX010000000
VERSION     DAOMAX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8944_length_2573_cov_7.399921"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..849
                     /locus_tag="PK742_12710"
     CDS             211..849
                     /locus_tag="PK742_12710"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HQU77498.1"
                     /translation="MNIAISAKKMLWWSLLLVYTFFCILMLGIVLQYIPAGPDTAFLG
                     IKQDYIELPWYLPAFYIHVFTAILALPAGFTQFQKKLRRKHPAVHRFNGRIYVFMILL
                     FGAPSGLVIGVYANGGVSSQIAFVLLAMLWWWFTWQAFVKAKKGLFRAHRIFMIRSFA
                     LTLSAITLRAWKYVIVAAFHPHPMDAYRIVAWLGWTLNLLIAELIIYQIRRP"
     gene            846..1724
                     /locus_tag="PK742_12715"
     CDS             846..1724
                     /locus_tag="PK742_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HQU77499.1"
                     /translation="MRTCLFLFPAILLASCAGNNERTGSEPDELSASLMLETNYIKPA
                     APVMVYGIEGYYTGFFEAEEFDANKNYMWSNKITVSIDSLSETTLYGHSVVAGNVRPF
                     SGTYNRADKTWQATVSEPGDDRYDGVFTFSVHSDSLVLRGTWTANDKKLAVTKRKYQL
                     EHRVFSYDPNIDLPEDVTYTELYGSYNEEDESVEAITEDALRFNPSVTLLKKEDIENM
                     YQGDLEVMRNSIYARHGYSFRNRRMRFLFDQLVDWYMPVSVNITGELTDLEKKNIDLL
                     KRYEAHAEKYYDVFGR"
     gene            complement(2197..2268)
                     /locus_tag="PK742_12720"
     tRNA            complement(2197..2268)
                     /locus_tag="PK742_12720"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2233..2235),aa:Glu,seq:ttc)
BASE COUNT          699 a          579 c          624 g          671 t
ORIGIN      
        1 cttcataatc ttcattcagg tcaaattcaa ttcggttctc attgagaacc tgagcctggt
       61 tttgaacgga aaagccaaag gtcaaagtgg ccagaagaag acttgctttc agttgcatga
      121 gtagtggttt gcgataaaaa taggattttc aattaattcg cagtacatta aatttattcg
      181 ttacatcctc tggatcatta atttagggtc atgaacatag caataagtgc taaaaaaatg
      241 ctttggtgga gcttgttgct ggtatatacc ttcttctgca tactgatgct tggaatcgta
      301 ctccagtaca ttcctgcagg acccgatacc gcctttctgg gtatcaaaca ggattatata
      361 gaactgccct ggtacctgcc tgctttttac atccatgtat ttactgccat cctggccttg
      421 cctgccgggt tcacccagtt tcagaagaag ttgcgccgga aacacccggc cgttcaccgt
      481 ttcaatggac gcatctatgt cttcatgatc ctgctgtttg gcgccccctc tggcctggtg
      541 atcggagtat atgcgaacgg aggggtctct tcacagatcg cctttgtgtt gttggccatg
      601 ttgtggtggt ggttcacctg gcaggctttt gtgaaagcca aaaagggatt attcagggcg
      661 caccgtatct tcatgatcag gagtttcgct ctgaccttgt cggccattac cctgcgggca
      721 tggaagtatg tgatcgtggc agcgttccat ccccatccga tggatgccta ccggatagtg
      781 gcctggctag gatggacact gaacctgctc attgctgaac tcatcatata tcaaatccga
      841 agaccatgag aacatgtctt tttttattcc cggccatact actcgcatcc tgtgccggca
      901 acaatgaacg gaccggatca gaaccggacg agctttcggc atcgctgatg ctggaaacga
      961 attatataaa acctgcagca ccggtgatgg tgtatggcat tgaaggttat tacaccggat
     1021 tttttgaagc cgaagaattc gatgccaaca agaactatat gtggagcaat aagatcacgg
     1081 tttctattga ttcactttct gaaacaacct tgtacggaca cagtgtggtt gcgggtaatg
     1141 tccgcccttt ttcgggcacc tacaacagag ccgacaagac ctggcaggcc accgtatctg
     1201 aaccaggtga tgaccggtac gacggcgtat tcacattcag cgtccacagt gattcactgg
     1261 tacttcgcgg aacctggaca gccaatgata agaaactggc ggtgaccaaa agaaagtacc
     1321 aactggaaca ccgcgtattt agctatgatc cgaatattga cttgcctgaa gatgttactt
     1381 atacggagct gtatggttct tataacgaag aagatgaatc agtggaagcg atcactgaag
     1441 atgcactgcg tttcaatcct tcggtaaccc tcctgaagaa agaagacatt gagaacatgt
     1501 accagggcga tctggaggtg atgcgtaatt ccatctatgc ccggcatgga tacagtttcc
     1561 gtaaccggcg tatgcgcttt ctgttcgatc agctggtaga ctggtacatg ccggtgtctg
     1621 tcaacatcac cggtgaactg accgatctgg aaaaaaagaa catcgacttg ctcaagcgct
     1681 acgaagccca tgcggagaag tattatgatg tattcggaag ataggaggga agtgggagtg
     1741 ggagtgggag tggacaagtg ccctgagaag gctccctcta cactcaaact cttaaagtag
     1801 cccgtaagag aagccctgcc tgcgtggctc agtcaggcaa ggaactcgag tttcaggaaa
     1861 gaaaaccggg ttgtttttta agccagttct taccagaggc tgttttcaga tatttttcac
     1921 gaaccctggc actgatgaaa tccggatgag attctgaaaa gaagatcttc cagggcctga
     1981 aggctttagt gtatctgttt tttcctttgt tatgttcttg caggcgccgg tctaattcgg
     2041 aggtcatacc aacatagact tctgaattca agtcattctt taaagcataa acgaaaacta
     2101 caggcataaa aaaagcaccc cgttctggag tgcatgtgcg gtggatgtag cccgtacgag
     2161 aatccctgga ttctcctatt gttaaaaaaa tctttgtagc ccgtacgaga atcgaactcg
     2221 tgtttcaaga atgaaaatct tgcgtcctaa cccctagacg aacgggccaa aatggctgtg
     2281 agccataaag agcaggcaac gatagccgat ttccgaattt tcttaaacaa atcggaaaaa
     2341 aaacaggaag gatattgggg gccacaggta aaaacagaag acttacatat ggaaattatt
     2401 cgcttcccgg atcataaaac agtggcaaaa cgcttattga acggatctga tttcaggaaa
     2461 gctgcttact gtaactgaag gcttgagaat ccctgcctga ctgcgtcacg caggcaggga
     2521 actcgtgttt caagaatgaa aatcttgcgt cctaacccct agacgaacgg gcc
//
LOCUS       DAOMAX010000353         2559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9026_length_2559_cov_2.971246, whole genome shotgun sequence.
ACCESSION   DAOMAX010000353 DAOMAX010000000
VERSION     DAOMAX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_2559_cov_2.971246"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PK742_12725"
     CDS             complement(<1..587)
                     /locus_tag="PK742_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQU77500.1"
                     /translation="MKKAYLDKGDNTGSPIGGNGVQTETKVAYIDGQAYAIHENETIL
                     SFIRRNLGRDLVPTLCDAPNLEPFGSCRVCSVDVALQANGAVRTVASCHTPVQPGYHI
                     SPSSENVQKLRKNIIELVLTDHPLDCLTCEVNGNCELQDVAAKVGIRKVRYPEGKTHL
                     DRQKDLSHPYMTSDLSKCINCYRCVRACDEVQGQFV"
     gene            complement(600..2249)
                     /locus_tag="PK742_12730"
     CDS             complement(600..2249)
                     /locus_tag="PK742_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQU77501.1"
                     /translation="MSKNLSELSGRKGLTDNLFEKLGTMAKGNGSPGVKEMEQLAEAF
                     LVGKANVYGSATFYDFTRPENQGKKVYVCNGSACMCAGTQAVVEQKLKETFAEEEIGV
                     MCCLGRCHENSAFHYQGNNYSGDDIKNIKTILDKPERRIEDYHVEAIGTPVLTEVFPG
                     LDAFYAPLKSWLGSDSASLLEEIRTSNIRGRGGAGFPMAIKLETCRNQDSKTKFIVCN
                     ADEGDPGAYSDRYLLEQRPHSVLLGMIMAGYVTGASWGVLYIRAEYPEAIDIVEKELE
                     KLYAAGLVGENILGTKFSFDFKVIHAQGAYICGEETALLSSIEGQRPEVRVRPPFPAQ
                     QGLFNKPTVVNNVETLAALPWILSHGGQAYAELGTERSKGTKLVSLDGHFNRPGMYEV
                     EMGTPLRKVIDELGQGFRHEVKAMHIGGPLGGLVPVSKIDDLTVDFESFSKNGFLLGH
                     ASVVCIPQDFPLVKYIEHLFEFTAHESCGKCFPCRLGSVRGAEMLDKAQHDGYKIDRT
                     LFDDLVETLEKGSLCALGGGLPLPVKNALQYFNEELSPYFK"
BASE COUNT          564 a          700 c          660 g          635 t
ORIGIN      
        1 acgaattgtc cctgtacttc atcgcaggca cgcacacagc gatagcagtt gatgcacttg
       61 ctgagatcgg aggtcatgta aggatgcgac aggtctttct gtctgtccag gtgggtcttg
      121 ccttccgggt aacgcacctt tctgatgccc accttcgcgg ccacatcctg gagctcgcag
      181 ttgccgttca cctcgcaggt gagacagtcc agcgggtgat ctgtcagcac cagttcaatg
      241 atgttcttcc gcagcttctg cacattctca gaagaaggtg agatatgata accgggctgc
      301 accggtgtat gacaggaagc tactgttctg accgcaccgt ttgcctgcaa tgccacatct
      361 accgaacaca cccggcagga gccgaatggt tccaggttgg gtgcatcaca cagcgtgggt
      421 accaggtcgc gaccgaggtt gcggcggatg aaagacagga tggtctcatt ctcatggatg
      481 gcataggcct gtccgtcaat gtaagccacc ttcgtttctg tctgcacacc gttccctccg
      541 atgggagatc cggtgttgtc gcctttgtct aaatatgcct ttttcatgcc tgaatattct
      601 tatttgaaat agggcgaaag ttcttcgtta aagtattgca atgcgttctt gaccgggagg
      661 ggaagtccgc cacccagcgc acacagagaa cccttctcga gtgtttccac cagatcatcg
      721 aagagggtgc ggtctatctt gtagccatcg tgttgtgcct tgtccagcat ctctgctccg
      781 cgaacggaac ccaatctgca ggggaagcac ttgccacagc tttcatgtgc cgtgaattcg
      841 aacagatgct cgatgtattt gaccagcgga aagtcctgcg ggatgcaaac cacagatgca
      901 tgaccgagca gaaaaccatt cttgctaaaa ctttcaaagt ccaccgtcag gtcatcgatc
      961 ttagaaacag gaacgagtcc gcctagtgga ccgccgatgt gcatggcctt cacttcatgt
     1021 cggaaaccct gccccagttc atcgatcact ttgcgcaggg gtgtacccat ttccacttca
     1081 tacatacccg ggcgattgaa atgaccatcc agcgaaacga gtttcgtgcc tttggatctt
     1141 tctgtaccga gttccgcata ggcttgtccg ccgtgactca atatccaggg cagtgcagcc
     1201 agtgtctcca cgttattgac caccgtgggt ttgttgaaga gtccttgctg ggcagggaag
     1261 gggggacgta cccgcacttc cggacgctgt ccttcaatgc tgctgagcag tgcggtttct
     1321 tcaccgcaga tgtaagcccc ttgtgcgtgg atgaccttga agtcgaaact gaatttggtt
     1381 ccgagtatgt tttctcccac cagaccggcg gcgtataatt tttcgagctc tttttctacg
     1441 atgtcgatgg cttccggata ctcggcacgt atgtacaaca caccccagct ggcaccggtt
     1501 acataaccag ccatgatcat gcccagcaac acactgtgcg ggcgttgttc cagcaggtag
     1561 cgatcagaat atgcaccggg gtcaccttca tcagcattgc agacgatgaa cttggtcttg
     1621 ctgtcctggt tgcgacaggt ttccagtttg atggccatgg ggaaaccggc accaccacga
     1681 ccacggatgt tcgatgtgcg gatctcttcc agcagggaag cagagtctga tcccagccag
     1741 gatttcagcg gcgcataaaa ggcatcgagg ccgggaaaga cttcggtcag cactggtgtg
     1801 ccgatggctt ccacgtgata gtcttcgatc ctgcgttccg gtttatccag aatggtcttg
     1861 atgttcttga tgtcatcgcc ggagtagttg ttgccctggt agtggaaggc gctgttctca
     1921 tggcagcgac cgaggcagca catcacaccg atctcttctt ccgcaaaggt ttctttcagt
     1981 ttttgttcta ccactgcctg ggtgccggcg cacatgcaag cggaaccgtt gcagacatag
     2041 accttctttc cctggttctc cgggcgggta aaatcataaa aggtggctga gccatagaca
     2101 ttggccttgc ctaccaggaa ggcctcggcc agttgttcca tctctttcac tccggggctg
     2161 ccattgccct tggccatggt tcccagcttc tcgaagaggt tgtctgtcaa ccccttgcgt
     2221 cccgacaatt cgctcagatt cttcgacatg aaaatgcagt ctttgagtaa tgtataaaag
     2281 taaggggaaa ggggcagaaa acaaaagtgg ccctaacccc attgtttctt cataggtgcg
     2341 ggtttaaacc cgcacctatg gagaatcctc cacacccacc cacactctct cccgcaccca
     2401 ttccctgtta gccgtcccgc cggtgttacc gggtgtggat gaatcaatca ataaagacaa
     2461 actaatacag aggaacgccg ctgcttcgca ttacacagtt ggataaacca cagagttcct
     2521 tggcaggtaa ctctgtaggg acgacagtgt gtaccccaa
//
LOCUS       DAOMAX010000354         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9067_length_2554_cov_3.519408, whole genome shotgun sequence.
ACCESSION   DAOMAX010000354 DAOMAX010000000
VERSION     DAOMAX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9067_length_2554_cov_3.519408"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="PK742_12735"
     CDS             complement(<1..2076)
                     /locus_tag="PK742_12735"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77502.1"
                     /translation="MRRILFFVLFWPVALLAQTPDPGEPGPYAVTSAEYDFGDEAFDP
                     PSFPEPVEVTGSVHYPDDLSDGPFPVILILHGRHSSCYNPGTGWTSIAWPCTAGYEPI
                     PSYTGYDYLAEQMASHGYIVISVSANSISSTDNSVGDYGMQARGELLQHHLDLWNDWN
                     TVGGDPFGTMFVGKLDMDHIGTLGHSRGGEGVIFHALYNRSLGSPYGIDAVLTLAPVD
                     FNRQTLTGIPLLNVAPYCDGDVSDLQGVHFYDDVRYEDPTDEAPKHSLMLPGGNHNFF
                     NTVWTPGLFPAGTADDWGYVDWTQSDDQCGTSDPGNGRYEPETQRNILLAYASAFFRF
                     YVGGEEDFSPILETNDIIPPASATIAPDDAFMSYHPSNRKKIDLNRLDNEGTESTNSL
                     GGAASQTGLTDYDICGDDFGEQYCLNLGASQEPHCKNGGITMLGLSQLGISWNSADDL
                     YDNELPVEWQNLSEFEYVQFRASVDFTGTASALDFSVELEDASGATASVNTDDYTNAL
                     FYPPGDIGTTLPRIMHHTIKLPLSAFEGIDLSAVTHIRMQFNGSASGSIFVSDLLISS
                     DENVYLPPVADFSVNVTTTCTGMVQFTDNTMLFPEEWLWDFGDGTTSDEQNPSHTYYT
                     SGDYTVTLTATNPAGSDEVTYEALIHVEHPIAPTAVGDVICAGEEATLSAMPAGGGTL
                     NWYDAPGGSL"
     gene            complement(2078..2455)
                     /locus_tag="PK742_12740"
     CDS             complement(2078..2455)
                     /locus_tag="PK742_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77503.1"
                     /translation="MKNLLLVLCLGIVSPLLAQQVDGPQLTGISETSAWTVIRIESDQ
                     PFIIGGNRYVLHVGDVVFEHSRHPDGNERIIEFLVDPDAWSAAPKGEDAVLVYGLYEG
                     NMTAQGRSEHMEGRYTALGPLTK"
BASE COUNT          586 a          657 c          725 g          586 t
ORIGIN      
        1 aagacttccg ccgggtgcat cataccagtt gagggttcct cctcctgccg gcatagctga
       61 gagcgtggct tcttctcctg cacagatcac atcgccaaca gcagttggag cgatcgggtg
      121 ttctacgtgt atcagtgctt cataagtgac ctcatctgaa cctgcaggat tggtcgcggt
      181 cagggtgacg gtataatcgc cgctagtgta gtaggtatgc gacggattct gttcatctga
      241 agtagttccg tctccaaaat cccacaacca ttcttccgga aacagcatgg tattgtcagt
      301 gaattgcacc atgcctgtac aggtggtggt gacattgaca ctgaaatctg ccaccggagg
      361 cagatagaca ttttcatcag agctgatcag caggtcgctc acaaaaatgc ttccggaagc
      421 agagccattg aactgcatac gaatatgggt cactgcactc aggtctatcc cttcaaaagc
      481 tgacaacggc agtttgatgg tgtggtgcat gatgcgtggc agggtggtgc cgatatctcc
      541 gggaggatag aacagagcat tcgtgtagtc atccgtattg acactcgctg tggcaccgga
      601 tgcatcttcc agttctaccg aaaaatccag tgcgctggcc gttccggtaa aatcgaccga
      661 agcacggaac tgcacatact caaattccga caggttctgc cattccacag gcaattcgtt
      721 gtcatagaga tcatcggcgc tgttccagct gatgcctaat tgcgataatc ccagcatggt
      781 gatgccaccg ttcttgcagt ggggttcctg agaagcgccg aggttgaggc agtactgttc
      841 gccaaaatca tctccgcaga tgtcgtagtc ggtcagtccg gtttgagagg cagcgccacc
      901 gaggctgttg gtcgattctg tgccttcatt atccagtcgg ttcagatcga tcttctttcg
      961 gttggaggga tggtaagaca tgaaagcatc gtcgggtgca atggtagcag atgccggagg
     1021 gatgatatcg ttcgtttcga ggatgggtga aaaatcctct tcgccgccta catagaatct
     1081 gaaaaatgca gaggcgtatg ccagcagaat attgcgctgc gtttcgggct cgtatcggcc
     1141 attgccggga tcggaggtgc cgcactggtc gtcgctctgg gtccagtcca catagcccca
     1201 gtcgtcggcc gttcctgcag ggaacagtcc cggagtccag accgtattga aaaagttgtg
     1261 gttgccaccg ggcagcatca ggctgtgctt gggcgcttcg tcggtggggt cttcatagcg
     1321 cacatcatca tagaagtgca caccctgcag atcagataca tcgccatcgc aatagggtgc
     1381 cacattgagc agcggaatac cggtcagggt ctggcgattg aaatcgaccg gagccagggt
     1441 cagcacggca tcgatgccgt agggacttcc cagcgaacga ttatagagtg catggaagat
     1501 gacgccttcg cctccgcggg aatgtccgag cgtaccgatg tggtccatat cgagtttgcc
     1561 gacgaacatg gtgccgaagg gatcgcctcc taccgtgttc cagtcgttcc agagatccag
     1621 gtggtgctgc agcagttcgc ctcttgcctg catgccatag tcgccgacgg agttatcggt
     1681 agaagagatg ctgttggccg agacagagat gacgatatac ccgtgactgg ccatctgctc
     1741 ggcgaggtag tcgtatccgg tgtaactcgg gatgggttca tagccggcag tacagggcca
     1801 ggcgatgctg gtccatcccg tacctggatt gtaacaggaa gaatgccgac cgtgcaaaat
     1861 gagaatgacc gggaagggac cgtcgctcag gtcatccgga tagtgcacag agcctgtcac
     1921 ctccaccggt tcggggaagg aaggcggatc aaaggcttca tcgccgaaat catattctgc
     1981 agatgttacc gcatagggac cgggttctcc cggatcgggt gtctgagcga gcaatgcaac
     2041 aggccaaaac aagacgaaaa acaggatcct gcgcatatca cttcgttaaa ggtccgagag
     2101 cagtgtagcg tccttccata tgctcagagc gcccctgtgc ggtcatgttg ccttcgtata
     2161 gcccatacac cagcacggca tcctctcctt tcggcgccgc cgaccaggca tcgggatcca
     2221 ccaggaattc gatgatgcgt tcattcccgt cagggtggcg actgtgttcg aacaccacat
     2281 cacccacatg cagcacatag cggttgccgc ctatgatgaa tggctgatct gattcaatgc
     2341 gaatgacggt ccaggcagaa gtttcagaga taccggtcag ctgcgggcca tccacctgct
     2401 gcgccagcag cggagatacg atacccaggc ataatacaag gaggaggttc ttcatcaggc
     2461 gggttttgag ttaactaata tagggaattc ttagggctgc tgtttcagcc aggttacaag
     2521 accgtctttc aattttgctg tttagcagta tcaa
//
LOCUS       DAOMAX010000355         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9135_length_2544_cov_4.468461, whole genome shotgun sequence.
ACCESSION   DAOMAX010000355 DAOMAX010000000
VERSION     DAOMAX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9135_length_2544_cov_4.468461"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK742_12745"
     CDS             complement(<1..925)
                     /locus_tag="PK742_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQU77504.1"
                     /translation="MADILLSNTYFLRFDPKQESNMSPYPPLATLYAASVLRDEGMDI
                     ALFDTQFAEGAEDAFPQIKAHAGKLFMIVDDGFNYLTKMCLVNMREACFTMIRYAKEQ
                     GCTVAVSSSDATDHMDAYLQEGADYIIVGEAEQTIRELVVCLDAKQPTDGVSGLAWSA
                     NGGVHKTLPRQVLTHLDSLPQPAWDLIDIQAYRSRWMQKHGYFSLNVATTRGCPFKCN
                     WCAKPIYGNRYNAHSAHYIMDQIELHVKQHGATHIWFCDDIFGLKPGWIHAFREEAER
                     RQLVFRFKIQCRADLLLNENNIADLAAAGCDE"
     gene            complement(918..1874)
                     /locus_tag="PK742_12750"
     CDS             complement(918..1874)
                     /locus_tag="PK742_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77505.1"
                     /translation="MHISHRHEHLPRQLSDIQSGVMSALVYFDLFRYPLTVPEIIRFS
                     PVRIDRLEQVEEALRELGSALIVFRFGDHYSLVNDIARVERRKLGNACAEKFWMKARR
                     MSVLMQRFPYVRSVNISGSLSKEYADDTTDIDYFIITAPGRIWLCRTLLALYKKVFLL
                     NSRKYFCINYFISEDDLSIPDKNLFSATEIVTLRNMSGQSIYARFLEENRWAFSHFPN
                     ASAADGMPVHTRGRRMQKAAERILAGRFGNLLDDLCFRITFYYWKRKFSWMREEEFAV
                     NIRSKRTVSKHHPQGFQFQVMERFAQRCAVLEAQHGIVFIHG"
     gene            complement(1892..>2544)
                     /locus_tag="PK742_12755"
     CDS             complement(1892..>2544)
                     /locus_tag="PK742_12755"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQU77506.1"
                     /translation="ILRPLLERRLHKTVRYQKYGLDMLIEPGVFHPGFFYSTEFLLQT
                     VRQRIAQQETVLELGCGSGLISLVLAADGAQVTAADINPVAVDALHRNADRNQIILEI
                     IQSDLFSALSGRCFHHILINPPFYPKDPANDAERAWFCGQDFSYFKRLFTDLPSYLQQ
                     DAQVWMVLSDGCDVKEISALAAQAHARLEEVATTVIRWERLWVFHIIYPNLTQGNV"
BASE COUNT          681 a          655 c          560 g          648 t
ORIGIN      
        1 tctcatcaca acctgcagca gccagatcgg caatgttgtt ttcattgagc agcaaatctg
       61 cacggcattg tatcttgaaa cggaatacaa gctgcctcct ttcagcttct tctcggaatg
      121 catgtatcca gccgggtttt aatccgaaaa tatcatcgca gaaccagata tgtgtagcac
      181 catgctgttt cacatgcagc tcgatctgat ccatgatata atgcgcacta tgagcattat
      241 acctgttacc atagatcggt ttggcgcacc agttacactt aaacgggcag ccgcgtgtgg
      301 tggccacatt gagtgaaaag tatccgtgct tctgcatcca tctgctgcga taggcttgaa
      361 tatctatcag atcccaggca ggttgaggaa gactgtccag atgggtcagt acctgacgag
      421 gcaatgtttt gtgcactccc ccattggcag accaagccag accgctcaca ccgtcagtcg
      481 gttgttttgc atccagacaa acaaccagtt cacgaatggt ttgctctgct tctcctacta
      541 tgatgtaatc agcaccttct tgcaggtaag catccatatg atcggttgca tctgagctgg
      601 aaacagccac tgtacagccc tgctctttgg catatctgat catggtgaaa caggcttccc
      661 tcatattgac cagacacatc ttcgtcagat agttgaagcc gtcgtccacg atcataaaaa
      721 gctttcctgc atgtgctttg atctgaggaa aggcatcttc tgcaccttca gcaaactgtg
      781 tatcaaacaa ggcaatatcc attccctcat cacgcaatac agatgctgca tataacgtag
      841 ccagcggcgg atatggtgac atgttcgact cctgtttcgg atcgaatcgc aaaaagtagg
      901 tattggagag cagaatatca gccatgtatg aatactatac catgttgtgc ttccagtaca
      961 gcacaccttt gtgcaaaacg ttccatcacc tgaaactgaa acccctgcgg atggtgtttg
     1021 ctaaccgtgc gcttcgaacg aatattaact gcaaactctt cctccctcat ccaggaaaat
     1081 tttcgtttcc agtaataaaa cgttatgcga aaacacaggt catcgagcag gtttccaaat
     1141 cttccagcca ggatcctctc tgcagccttc tgcatgcgtc tgccacgggt gtgaacaggc
     1201 ataccatctg cagcagatgc attgggaaaa tggctgaaag cccatctgtt ctcctccaga
     1261 aagcgggcat atattgactg accactcatg ttgcgcagtg taacgatctc tgtggcagag
     1321 aacagattct tgtcaggaat ggacagatca tcttcactga tgaaatagtt gatgcagaag
     1381 tattttctgc tgttgagcag aaagaccttc ttatacaacg caagcagcgt gcggcacaac
     1441 cagatacgcc cgggtgcggt gatgatgaaa tagtctatat cggttgtatc atcggcatac
     1501 tcctttgaca gggaaccgga aatattcaca cttcgcacat aaggaaaacg ctgcattaaa
     1561 acagacatac gccttgcctt catccagaac ttttcagcac aggcatttcc cagcttacgc
     1621 ctctccaccc gggcaatatc atttaccaac gaataatgat cgccgaaacg aaaaacgatc
     1681 agagcagatc ccagctccct taacgcctct tccacctgct ccagacgatc gatccttacc
     1741 ggcgaaaaac ggatgatctc cggcacagta agcgggtatc tgaacagatc aaaatacacc
     1801 agtgccgaca tgaccccaga ctgaatgtca gacaattgcc ggggcagatg ttcatgccgg
     1861 tgactgatat gcatacgtac gaagttaact gttacacatt tccctgtgtc agattcgggt
     1921 aaatgatgtg gaacacccac aatcgttccc atctgatcac ggtggtagct acctcttcca
     1981 gacgggcatg cgcctgtgcc gcaagtgcac tgatctcttt cacatcacat ccgtcagaca
     2041 ataccatcca cacctgagca tcctgctgca gataggaagg caggtcagtg aacagtcttt
     2101 tgaaatagga aaaatcctgt ccgcaaaacc atgctctttc tgcatcgttt gcaggatcct
     2161 ttggatagaa tggcggattg atgagtatat gatgaaagca tctgcctgac agtgcactga
     2221 acagatcgct ttgtatgatc tcgagaatga tctgatttcg gtcggcattg cgatgcaatg
     2281 catccacagc aactggattg atgtctgcag cggtcacctg cgcaccatcg gccgcgagta
     2341 ccaggctgat gagtccgctt ccgcaaccaa gttccagcac ggtttcctgt tgtgctatcc
     2401 gttgccttac cgtttgtaaa agaaattcgg tactgtagaa aaaaccggga tgaaaaacgc
     2461 ctggttcgat cagcatgtcc aggccatatt tttgataccg gactgtttta tgcaacctcc
     2521 tttcaagcag cggtctcagt atat
//
LOCUS       DAOMAX010000356         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9285_length_2523_cov_4.048217, whole genome shotgun sequence.
ACCESSION   DAOMAX010000356 DAOMAX010000000
VERSION     DAOMAX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9285_length_2523_cov_4.048217"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PK742_12760"
     CDS             <1..1479
                     /locus_tag="PK742_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQU77507.1"
                     /translation="NIQNILNFEKDFPEVKTFRLEQNYRSTKVIVEAANQIIGNNKMQ
                     LAKKIWTSNDEGDRIKVIKAASDNEEGRLVADAIFEQRMRDHFVNNDFAILYRTNAQS
                     RAFEESLRRLNIPYRIYGGISFYQRKEIKDLMGYLKLTVNPHDEEAFKRVVNYPARGI
                     GKTTMEKIIVFAADNDRSLWDIAENIALFPFPAGTRKSITEFVTMIRSFQAMLEKKNA
                     YEVASHIAKSTGILKELYNDQSVEGLSRYENIQELLNSISEFTDPGDNPAFPPDATEA
                     DKSLAAYLQQVSLLTDADQEEGQEDSVKLMTVHAAKGLEFKSVFIVGMEENLFPSGLS
                     TNTREELEEERRLFYVAVTRAEKKLTITHAATRYKYGSLDYCEPSRFLEELPQNLLEM
                     VGYHRSKSSAWEEKVVYQQPKISRPEPVKTAYVHAVSSGFNPAPPERLSAGMAIEHQK
                     FGYGKIVSLEGQNDGRMATIDFDKAGQKKLLLKFAKLSIVQN"
     gene            1466..2194
                     /locus_tag="PK742_12765"
     CDS             1466..2194
                     /locus_tag="PK742_12765"
                     /inference="COORDINATES: protein motif:HMM:NF040370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77508.1"
                     /translation="MSRTEPIRINLWSGPRNISTALLYSFAQRADTRVLDEPLYGWYL
                     QETDMQHPGREDILNSMECDGQKVIAEQLMGTLDKPVLFAKQMTHHLLDLPKDFMLAG
                     KNVLLIRHPAKVLMSYSKVIDNPTLDDIGIRQSSELFEYLTAHQTHVYVADGDDILKD
                     PATQLEKLCEACSIAFDPAMLQWPAGKRPEDGVWARYWYDRVHRSTGFEPYAEKDVVL
                     PDELKGLAEEAMVYYRKLRFQSPG"
     gene            complement(2238..>2523)
                     /locus_tag="PK742_12770"
     CDS             complement(2238..>2523)
                     /locus_tag="PK742_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77509.1"
                     /translation="PCDTLNETNSVAEVTSPPFKAYPNPVQDHLHIDNPLPTTCQAVV
                     YNAMGQEVERFDLDPGGQAHATGWWPVGFYSLVLQQPDGTLDKISLIKME"
BASE COUNT          669 a          650 c          674 g          530 t
ORIGIN      
        1 aacatccaga acatcctgaa ctttgaaaaa gacttcccgg aagtgaagac cttccgcctg
       61 gaacagaact accgaagcac caaggtcatc gtggaagctg ccaaccagat catcggcaac
      121 aacaagatgc agctggccaa aaagatctgg acctcgaacg atgagggcga ccgcatcaag
      181 gtcatcaaag ctgccagcga taatgaagaa ggacggctgg tggcagatgc catctttgag
      241 cagcgcatgc gggatcattt cgtgaacaac gacttcgcca tcctctaccg caccaatgcg
      301 cagtcgcggg catttgaaga atcactgcgc cgtctcaata ttccctatcg catttacgga
      361 ggcatctcct tctatcagcg caaggagatc aaagacctga tgggctatct caagctgacg
      421 gtcaatccgc acgacgaaga agcttttaag agagtggtca actatcccgc aagaggcatc
      481 ggcaaaacga ccatggaaaa gatcatcgtc ttcgctgctg ataatgatcg ttcgctgtgg
      541 gacatcgccg agaacatcgc cctcttcccc ttccccgcag ggaccagaaa atcgatcacc
      601 gagttcgtga ccatgatacg cagtttccag gctatgctgg aaaaaaagaa tgcctacgaa
      661 gtggcctcgc atattgccaa gagcaccggt atcctgaagg agctgtacaa cgaccagagt
      721 gtggaaggac tgagccgcta tgagaacatc caggaattgc tgaactccat cagcgagttc
      781 accgatcctg gcgacaaccc cgcttttccg cccgatgcca ccgaagctga taaaagcctg
      841 gccgcttacc tgcagcaggt gtcattgctg accgatgcag atcaggaaga aggccaggaa
      901 gacagcgtga agctgatgac cgtgcacgcc gccaaaggac ttgaattcaa aagtgtgttc
      961 attgtgggga tggaagaaaa tctcttcccc agcggactca gcaccaacac ccgcgaagaa
     1021 ctggaagaag agcgaaggct attctatgtg gctgttaccc gtgccgagaa aaagctgacc
     1081 atcacccatg ctgccacacg ttataaatac ggcagtcttg attattgtga gcccagccgc
     1141 ttcctggaag aactgccaca gaacctgctg gagatggtgg gctatcaccg gtccaaatca
     1201 tctgcctggg aagagaaggt ggtgtatcag cagccgaaga tcagccgtcc ggaaccggtg
     1261 aagacagcct atgtgcatgc ggtctcttcg ggtttcaatc ccgcaccgcc cgaacgactt
     1321 tctgccggta tggccatcga acaccagaag ttcggctatg gcaagatcgt gagtctggaa
     1381 ggacagaacg acgggcgcat ggccaccatt gatttcgaca aggccggaca gaaaaaactc
     1441 ctgctgaaat ttgccaaact gagcattgtc cagaactgaa ccaatacgca tcaacctgtg
     1501 gagcggtccg cggaatatct ctacggccct cctgtacagc tttgcccaac gggcagatac
     1561 aagggtactc gatgaaccat tgtacggctg gtacctgcag gaaacagata tgcagcatcc
     1621 gggaagggaa gatatcctga acagcatgga atgcgacggg cagaaggtca ttgcagagca
     1681 gttgatgggt acactggaca agccggtgtt gttcgccaaa cagatgaccc accacctgct
     1741 ggatctgcca aaggatttta tgctggcggg gaagaatgta ttgctgatac gccatccggc
     1801 gaaggtgctc atgtcataca gcaaggtcat cgacaacccg acgttggacg atatcggcat
     1861 acggcagagc tcggagttgt tcgaatatct tacagcgcat cagacgcatg tgtatgttgc
     1921 cgatggtgat gacattttaa aagacccggc cacgcagctg gaaaagttgt gcgaagcctg
     1981 ttccatcgct ttcgatcctg ccatgctgca atggcctgcg ggcaaaagac ctgaagatgg
     2041 tgtttgggcc aggtattggt atgaccgtgt acaccggagc accggctttg agccttacgc
     2101 agagaaagat gtcgtgctgc cggatgaact gaagggtttg gcagaagagg cgatggtgta
     2161 ttaccgtaaa cttcgtttcc aaagtcccgg gtaaggtgat caggaattat ctgtgctgga
     2221 tgacagcatt tgttgtttca ttccatttta atcagggata tcttatccaa agttccatcc
     2281 ggttgctgca acaccaagga ataaaaaccc accggccacc agcctgttgc atgtgcctgc
     2341 ccacctggat ccaggtcaaa ccgctccact tcctgcccca tggcattgta caccactgcc
     2401 tgacaggttg ttggcagcgg gttgtctatg tgcaaatgat cctgcaccgg gttggggtag
     2461 gctttgaagg gaggggatgt tacttctgca acagagttgg tttcatttaa cgtatcgcaa
     2521 ggg
//
LOCUS       DAOMAX010000357         2490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9489_length_2490_cov_4.109240, whole genome shotgun sequence.
ACCESSION   DAOMAX010000357 DAOMAX010000000
VERSION     DAOMAX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9489_length_2490_cov_4.109240"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..626)
                     /locus_tag="PK742_12775"
     CDS             complement(171..626)
                     /locus_tag="PK742_12775"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQU77510.1"
                     /translation="MHSSNTTSILREVYLAEIDKCLQELEQYPSDASLWTLAPGTLNS
                     GGNLILHLAGNLLHFIGHTLGNSGYVRDREREFGAKDLSREQLRQELVEAREQVDRVL
                     NGLYDQQLDEPYPIREFKGGKTIGYVLQAFLAHFSYHLGQLNYHRRITT"
     gene            complement(613..1611)
                     /locus_tag="PK742_12780"
     CDS             complement(613..1611)
                     /locus_tag="PK742_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQU77511.1"
                     /translation="MIRRKQRSPLSKIYIALVLVGLTLSFGTGGYMLIEQYNFVEALY
                     MTVITLATVGFQEVHPLSASGRVFTTILILFNLGIFAFVITQISRYFLDGEFARTYKI
                     YKMRNAIDKLENHTIICGFGRNGQEAVRILERNREPFVVIEQDEQILEDKKYDDLLYI
                     RADATRDEVLQEAGIERAKALISALPDDTLNVFVILTARSLNTQVTLISRASENTSVR
                     KLKNAGADNVIMPDKIGGAHMAMLVTNPDIEEFVDMMATHSGDDFQISELYINSSVLL
                     RELNCWERTGATLLGYKDSNQEFQLNPSPDTTVEAGSSIIVMGSKHQINQLKHALQ"
     gene            1685..2407
                     /locus_tag="PK742_12785"
     CDS             1685..2407
                     /locus_tag="PK742_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQU77512.1"
                     /translation="MDFSIFLTTDAWLALITLTVLEIVLGIDNIIFISILSGKLPASQ
                     QKRARRLGLALAMITRVLLLLSLSWIMGMEKELFSVLGKGISGRDLILLLGGLFLLYK
                     ATTEIHSKLEGEEEEHKTVRVPSFNGVIVQILLLDIVFSLDSVITAVGMADEVLVMII
                     AVVIAVLIMLWAADPISDFVNKHPTVKMLALAFLVMIGVSLLIEGWGEHIDKGYIYFA
                     MGFSVLVEMLNLQAKKRRKGSV"
BASE COUNT          598 a          562 c          651 g          679 t
ORIGIN      
        1 gggtttgggg tagcctttcg ttttgacctt atcggtatgc aggatctctc cattacgggt
       61 taccagcccg aatttgatcc cggttccacc tatgtctata cctaaaacaa cgtcctgtgc
      121 catagcaggt acaaagtaaa tacattcagt gaaaccccgg tcacggttgt tcaggtggtg
      181 atccgccgat gataattcaa ctgacccaaa tggtagctga agtgtgccag aaatgcctgc
      241 agcacatatc cgatggtctt tccacccttg aattcgcgga tcggataggg ctcatccaac
      301 tgctgatcat acaggccgtt gagtacacga tctacctgtt cacgggcttc gaccagttcc
      361 tgtctgagct gttcacggct caggtctttg gcaccgaatt cccgttcacg gtctcttaca
      421 tagccgctgt taccaagtgt atgaccaata aaatggagca gattacctgc caggtgcagg
      481 atcaggttac ccccgctgtt gagcgtgcct ggcgcaagtg tccagaggct ggcgtcagaa
      541 ggatactgtt ccagttcctg gaggcattta tcgatctctg cgagatagac ctctcggagg
      601 attgaagtag tgttactgga gtgcatgttt caattggttg atctgatgtt tggaacccat
      661 tacgatgata ctgctgccgg cttcaacggt tgtatcggga gaagggttca gttgaaattc
      721 ctggttgctg tctttgtaac ccagtaatgt ggctcctgta cgttcccagc agttcagctc
      781 acgcaagaga actgaactgt ttatatacag ttcgctgatc tggaagtcat cgccggaatg
      841 ggtggccatc atatccacga attcttcgat atctgggttg gtaaccagca tggccatatg
      901 tgcaccaccg atcttatccg gcatgatgac attatctgcc ccggcattct tcagtttgcg
      961 aacagaagta ttttcagaag cccggctgat cagggtaacc tgtgtgttaa ggctgcgggc
     1021 agtcagtatg acaaacacat tgagtgtgtc atcgggcaaa gcagatatca gggccttggc
     1081 ccgctctatg ccggcttcct gcaacacttc atcacgggtg gcatctgcac gaatatataa
     1141 cagatcatca tattttttat cttccagtat ttgttcgtcc tgttcaatga ccacaaacgg
     1201 ctccctgttt ctttccagga tgcgaactgc ctcctgacca tttctgccaa agccgcatat
     1261 aatggtatga ttctccagtt tgtctatggc gtttctcatt ttgtagattt tataagtacg
     1321 ggcgaattct ccgtccagga aatagcgtga tatctgggtg atcacaaagg caaaaatacc
     1381 caggttgaac aggatgagga tggtggtgaa taccctgcct gaagcactga gggggtgcac
     1441 ttcctgaaag ccgacggttg ccagggtaat gaccgtcata taaagtgctt ccacgaagtt
     1501 gtattgctca ataagcatat agcctccggt gccgaaagac agggttagtc ctaccagcac
     1561 cagggcgata tatatcttag acagcggcga ccgttgtttc cttcggatca tatgcggtaa
     1621 aaatagttga aaactaaaat ggcttcggtg atttacatgc tacttttacc gaataaacat
     1681 caacatggat ttcagcatat tcctgacaac cgatgcctgg ctggccctta ttacactgac
     1741 cgtactggag atcgtgttgg gcatagacaa cattattttc atttctatcc tgtctggaaa
     1801 gctgcctgcc tcacagcaaa agagagcccg tcgtctgggt ctggcactgg ctatgatcac
     1861 cagagtgctg ctgctgttat cgctcagctg gatcatgggt atggaaaaag agctgttctc
     1921 tgtgctgggc aaaggtatca gcggccgtga cctgatactg ctgctgggtg gattattcct
     1981 gctatacaag gcaacaacag agatccacag caagctggaa ggagaagaag aagaacataa
     2041 aacggtcagg gtgccatcct ttaacggggt gatcgttcag atcctgctgc tcgacattgt
     2101 cttttctctg gattcagtga tcactgctgt tggaatggct gatgaagtac tggtcatgat
     2161 cattgcggtg gtcattgccg tgcttattat gttatgggcc gccgatccca tttctgattt
     2221 cgtgaataaa caccccacgg tcaagatgct ggcactggca tttctggtca tgatcggggt
     2281 gtcactgctg attgaaggct ggggtgaaca tatagacaag ggctatatat attttgcaat
     2341 gggattttcc gtgctggtgg agatgctgaa cctgcaggct aaaaaaagaa ggaaagggag
     2401 tgtgtgagtg ggagtgtgag tgggagtgtg agtgggagta ggagtaagag tgggggtgtg
     2461 agtgggagtc ttccattgcc tgacccggca
//
LOCUS       DAOMAX010000358         2466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9631_length_2466_cov_4.795106, whole genome shotgun sequence.
ACCESSION   DAOMAX010000358 DAOMAX010000000
VERSION     DAOMAX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9631_length_2466_cov_4.795106"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(505..1848)
                     /locus_tag="PK742_12790"
     CDS             complement(505..1848)
                     /locus_tag="PK742_12790"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQU77513.1"
                     /translation="MPVKKRSEEKKIFVLDTSVILHDHNSVKKFEEHDVVIPITVLEE
                     LDNFKKGNETINFEAREFIRFLDQKSTEGTLQDWVPINGKSTGLMKVMMFEENNGVNA
                     EVVFGEEKADHKILNTALVVKQQHPGRKVILITKDINLRLKAKALGLVSEDYKNDKIV
                     DVKFLRDNVETIEGISSGTIDKIYAEGHIGFKQILKEEPFANQCFVLKNGSSSVLASY
                     NKDEQIFERVNKSSAYGIKPRNAEQVFALNAILNPNIKLVALQGISGSGKTLLALAGA
                     LEQRRNYRQVFLARPIVPLANKDIGYLPGDIKSKLNPYMEPLWDNLKMIQSQFDSHDS
                     EFKAINQMIETEKLVIQPLAYIRGRSISNIFFIIDEAQNLTPLEVKTIITRAGENTKI
                     IFTGDVNQIDTPYLDSQSNGLSFLIDKIKNHPMCSVITLEKGERSELANLANELL"
     gene            1847..2035
                     /locus_tag="PK742_12795"
     CDS             1847..2035
                     /locus_tag="PK742_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77514.1"
                     /translation="MQIDYAINNTSLNLQCKPESAPSNVEKHKVVAESPQGLQLSLFI
                     ERTEKSFLLPYAELLITG"
     gene            complement(2038..>2466)
                     /locus_tag="PK742_12800"
     CDS             complement(2038..>2466)
                     /locus_tag="PK742_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQU77515.1"
                     /translation="KEAWDFLSSPANLQAITPDYMGFQIRSGFSEGEKMYAGMIIEYT
                     VRPVLGIPLHWVTEITHVQEPEYFVDEQRFGPYALWHHKHFIRPVEGGVEMEDVVHYK
                     LPLGWLGKLAHMLFVKRQLRQIFDFREKKLTELFGQSLRV"
BASE COUNT          635 a          594 c          551 g          686 t
ORIGIN      
        1 tgagggtatc aggttatatg agataccatg attcccccct ggctgcagag ttccatgcgg
       61 gtaactctgc agggacattt aaatcagata atatttgtag gaataataaa aaaaaaggca
      121 gccaccaggc acgacattaa cacaccgatc attccgatat aaaacataaa tgtggtaaag
      181 accttataaa atccatgatt ctttttgctc acataataag atgagatagc cgttcctaat
      241 gaaaccatca atgtaaccaa ccaaaacact tttctacccg ggtatgtaac tctatatgcc
      301 tcaaaagaat ccgccgtaga agaagaaatc aggctctgat caaatccata aacaaaaaac
      361 aatatctcgt ggagaaaaaa ccagtatccc aaggagcata ggacaaaaag ggtaactagt
      421 aacaaatatg ataaactgat acgcatacct aattaagaaa tcgcagcacg ctcctgtcac
      481 attgctaaaa cccgatatgg ataatcacag caactcgttc gccaggttgg ccagttcgct
      541 gcgttcgcct ttctcgaggg tgatgacaga acacatcggg tggttcttga tcttgtcgat
      601 caggaagctc agtccgttgc tctgtgaatc gaggtaaggc gtatcgatct ggttgacatc
      661 gcctgtgaag atgatcttgg tattctctcc tgcacgggtg atgatggtct tgacctccag
      721 cggcgtgagg ttctgcgctt catcgatgat gaagaagata ttactgatac ttcgtccgcg
      781 gatatatgcc agcggctgga tcaccagttt ctcagtttcg atcatctggt tgatggcctt
      841 gaattcactg tcgtggctgt cgaactgact ctggatcatc ttgaggttgt cccaaagtgg
      901 ttccatataa ggattcagtt tcgacttgat atcgcccggc aggtaaccga tgtccttatt
      961 ggccagcggc acgatgggac gagccaggaa tacctggcgg taattgcgtc tttgttccaa
     1021 agcacctgcc agagccagca aggtttttcc gcttccgctg ataccctgga gggccaccag
     1081 cttgatattc ggattcagaa tggcgttcag tgcaaatacc tgctcggcgt tgcgtggttt
     1141 gatgccatag gcactgcttt tattgacccg ttcaaatatc tgttcgtctt tattatagga
     1201 tgccagcact gaactgctgc catttttcag cacgaaacac tgattggcga aaggctcttc
     1261 tttcaggatc tgtttgaagc cgatatgccc ttctgcatag atcttgtcga tggtgccgct
     1321 gctgatgcct tcgatggtct cgacattgtc gcgaaggaat ttcacatcca cgatcttgtc
     1381 gttcttgtaa tcttctgaaa caagacccaa agcctttgct ttcagacgca ggttgatgtc
     1441 ttttgtaatg aggatgacct tcctgcccgg gtgttgctgt ttcacgacca gtgcagtatt
     1501 caggatcttg tgatcggctt tttcttcgcc gaagacgacc tcggcattga cgccgttgtt
     1561 ctcttcgaac atcatgacct tcatgagccc tgtggactta ccattgatgg gcacccagtc
     1621 ctgcagggta ccttcagttg atttctgatc aaggaatcgg atgaattctc tcgcttcaaa
     1681 gttgatggtc tcgtttcctt tcttgaagtt gtccagttct tccagcaccg tgatcgggat
     1741 caccacatcg tgctcttcaa atttcttgac agaattgtgg tcgtggagga tgacggaagt
     1801 gtctaggaca aagatcttct tctcttcaga acgctttttt accggcatgc agatcgatta
     1861 tgccatcaat aataccagtc tgaatcttca atgcaaacct gagagtgcac cctctaatgt
     1921 ggaaaagcac aaagttgtag ctgaaagccc gcaagggctg cagctgagcc tgttcataga
     1981 aaggacagaa aaatccttcc tgctgcctta tgcagaatta ttaataaccg gttaatatca
     2041 gacacgaagt gactggccaa acaattccgt cagtttcttc tcccggaaat caaatatctg
     2101 cctgagctgt ctttttacaa aaagcatatg tgccagttta cccagccagc ccagcggcaa
     2161 tttgtaatgc accacatctt ccatttccac tccgccttcc accggcctga taaaatgttt
     2221 gtgatgccag agtgcatacg gaccaaacct ctgttcatcc acgaaatatt caggttcctg
     2281 tacatgtgtg atctcggtga cccagtgcaa cggaataccc agtaccggtc tgaccgtgta
     2341 ttcgatgatc atgcctgcat acatcttctc tccttcactg aacccgcttc tgatctgaaa
     2401 gcccatgtag tcaggagtga tggcctggag gtttgcaggt gatgacagga aatcccacgc
     2461 ttcttt
//
LOCUS       DAOMAX010000359         2462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_9659_length_2462_cov_3.619859, whole genome shotgun sequence.
ACCESSION   DAOMAX010000359 DAOMAX010000000
VERSION     DAOMAX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_2462_cov_3.619859"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PK742_12805"
     CDS             <1..1588
                     /locus_tag="PK742_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU77516.1"
                     /translation="LLLSARRSYLQFLFDLIGLPFLPTYNDFQLKHKIRFDQHNELTI
                     VGLGAIDQFDLNTQDDTSAFQQYILGNLPVQTQWNYATGAVYKHYQPRGAITLVASRN
                     MLNNRAYKYLGNDESVPSNLTFDFNSQEIENKLRFEENLRISGFKINGGLNYEYARYN
                     NSTFQLLASGTDPVSFSYSSELDLHKWGVFGQVSRSVLNELVDLSFGFRADANSYSAD
                     MNNLLDQFSPRFSVSYRFLPEWSLNANTGIYYQLPAYTTLGFRDEAGELVNKDNGLSY
                     IRGKHLVAGVEFRPEPATRFSVEGFYKLYDRYPFTLADSISLANLGADFGVIGDEAVV
                     STGKGRAYGAELLAQQKLKKGFYGILAYTLVVSEFTDKNGNYVPSSWDSRHIIALTGG
                     KEFQKNWQLGVKWRFSSGQPYTPWDVATSSLTYLWDASGTGILDYDSLNTLRLTATHQ
                     LDLRIDKKWFFERMNLNLFMDVQNVYNFQAQLPPLLDVVRDMDGTPVLQPDDPGRYQT
                     YFLDNPSGTVLPTIGIIIEI"
     gene            complement(1585..>2462)
                     /gene="rfaD"
                     /locus_tag="PK742_12810"
     CDS             complement(1585..>2462)
                     /gene="rfaD"
                     /locus_tag="PK742_12810"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQU77517.1"
                     /translation="DDHSRPEKASNLSGKRFIVKMDRELFPHTLAAQADQIEAVLHIG
                     ARTDTTEQDVALLDRLNTTYSKQVWEFCTAFQIPLIYASSAATYGDGSKGYSDAPERT
                     PGLEPLNPYGRSKQLFDEWVLQQEHSPPFWAGLKFFNVYGPNEYHKGRMASVIFHAFR
                     QISETGQVKLFRSHKPEYADGYQLRDFIYVEDVVRVISWLMENRPASGIYNLGTGQAR
                     AFYDLAKATASAMNKELVVDWIDIPEDIRDTYQYFTEADMNKLRLAGYRDVFHSLEDG
                     IGDYIQQYLIPGKYR"
BASE COUNT          625 a          605 c          583 g          649 t
ORIGIN      
        1 cctgttatta tcggccagaa gaagctacct gcagttttta ttcgacctca tcgggctgcc
       61 tttcctgcca acctataatg atttccagct caagcataag atccgctttg atcagcacaa
      121 tgagctgacc attgtagggc tgggagccat cgatcagttt gatctgaaca cccaggatga
      181 cacctctgct tttcaacaat atattctggg caacctgcct gtacagacgc aatggaacta
      241 tgccaccggt gctgtgtaca aacattatca accaagggga gccatcactt tggtggccag
      301 caggaacatg ctgaacaacc gggcatacaa gtacctcggt aatgacgaaa gtgttccttc
      361 caatctgacg tttgatttca attctcagga gatagagaat aagctgcgat ttgaagaaaa
      421 tttacgcatc agcggcttca agatcaacgg tggactgaac tacgaatatg cacggtataa
      481 caacagtaca tttcagttgc tggcatccgg aactgatccg gtatcatttt catacagctc
      541 tgaactggac ctgcataagt ggggtgtatt tggtcaggtg agcagaagcg tgctgaacga
      601 actggttgat ctttcctttg gatttcgggc agacgccaac agctatagtg ctgacatgaa
      661 caacctgctg gatcagttca gccccaggtt ttctgtaagt tacagattcc ttcctgagtg
      721 gagcctcaat gccaacacgg gtatctatta tcagttacct gcctatacca ctttgggctt
      781 tcgcgatgag gcaggtgagc tcgttaacaa agacaacgga ctgagctaca ttcgtggaaa
      841 acacctggtt gcaggagttg aatttcggcc ggaacctgct acccggtttt cagtagaagg
      901 attctacaaa ttatatgacc gctacccttt cactctggca gactccatat cactggctaa
      961 cctgggtgcc gattttggcg tgataggcga tgaagctgtt gtaagcaccg gaaaaggccg
     1021 ggcctatggc gcagaattac tggctcagca aaaactcaaa aagggttttt atggcatact
     1081 ggcctacaca ctagtggtca gtgaattcac agataaaaat ggcaactatg tacccagcag
     1141 ctgggacagc cggcatatca ttgcactgac aggaggcaag gaattccaaa agaactggca
     1201 gctgggcgtc aagtggcgat tcagcagcgg ccaaccttat accccatggg atgtagctac
     1261 ttcctcactg acctatctgt gggatgccag cggtacaggg atcctggatt atgacagcct
     1321 gaataccctt cgcctgactg caacccatca gcttgatctg cgcattgaca aaaagtggtt
     1381 ctttgagcga atgaacctca accttttcat ggatgtacag aatgtatata atttccaggc
     1441 acagcttcca ccattgctgg atgttgttcg tgatatggac ggaacacctg ttctgcaacc
     1501 ggatgatcct ggcaggtatc aaacatattt tctggataac ccgtcgggaa ctgtactgcc
     1561 aacaattgga attatcatag agatctagcg gtactttccc ggaatcagat actgttgtat
     1621 ataatcaccg atgccgtctt ccagtgaatg gaatacatcc cggtatcctg ccagtctgag
     1681 tttattcata tcggcctctg tgaagtattg ataggtatct cgaatatctt ccggaatatc
     1741 tatccagtca acaaccagct ctttgttcat agccgaagca gttgccttcg ccaggtcata
     1801 gaatgctctt gcctgtccgg ttcccaggtt atagatgccc gatgcaggcc tgttttccat
     1861 cagccagctg atgaccctca ctacatcctc cacatagatg aaatcacgca gctggtaacc
     1921 atctgcatac tccggtttgt gtgaccggaa aagtttcacc tgacctgttt cactgatctg
     1981 acggaatgca tggaagatga cgctggccat tcggcctttg tggtactcat tggggccata
     2041 cacattaaaa aatttcaatc ctgcccagaa agggggagag tgttcctgtt gcagcaccca
     2101 ttcatcgaac agctgtttgg agcgtccata gggattgagc ggctccagtc cgggcgttct
     2161 ttcaggcgca tcactgtagc ccttgcttcc atctccgtaa gtggcagcag atgaagcgta
     2221 aatgagtggt atctgaaaag cggtacagaa ttcccaaacc tgtttcgagt aggttgtatt
     2281 cagcctgtcc agcagtgcca cgtcctgttc ggtcgtatca gtccgggccc ctatgtgcaa
     2341 cactgcttct atctgatcag cctgagcagc aagcgtatgc ggaaacaact cccggtccat
     2401 ttttacgatg aatctttttc cgctgaggtt ggaagctttt tccggtctgc tgtggtcatc
     2461 ta
//
LOCUS       DAOMAX010000360         2406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10075_length_2406_cov_4.069758, whole genome shotgun sequence.
ACCESSION   DAOMAX010000360 DAOMAX010000000
VERSION     DAOMAX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_2406_cov_4.069758"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PK742_12815"
     CDS             complement(<1..819)
                     /locus_tag="PK742_12815"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQU77518.1"
                     /translation="MKRFSLMIMACSLAGMVSAQSAQYPVDGNTVTFIHGNNDPRPAV
                     QEALRNLDNWQDFTAAHGNWWVTFNERSAMPHRATGQGIATTGTTPEERAMHFLQTEL
                     NGFGMQGVQLALNAVNTSKYDYVSFRQQYEGLDVLFSNATLRMDPATGKVIMFGLDVF
                     PAMELNTVPVLTPEAAANFATSGLDVTVESSTALPVLKILPVPVTEATSSETVGSGYA
                     YKLVYEVDVHTTDFENLPGNYYTLVDAHTGAVYYRTNKVHACGSYLMTADAEVQA"
     gene            complement(911..1681)
                     /locus_tag="PK742_12820"
     CDS             complement(911..1681)
                     /locus_tag="PK742_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU77519.1"
                     /translation="MKLTFLGTGTSGGVPMIGCSCQVCRSEDARDKRLRSSVMLEEGE
                     TRVVIDCGPDFRQQMLREDVHRLDAIVFTHGHKDHTAGMDDVRPFNYFERKAIPLYVD
                     ELTEEALRKEYHYVFGTNTYPGIPQVSLHRLTGKPFDIGAIHLEPIRVMHLDLPVFGF
                     RIGKLAYITDANRIDEAAMNRLRDLDILVINALRMTPHISHFSLPEALELIRELRPGS
                     AYITHISHQMGTHASVEAGLPENVHLAYDGLRLEAAGF"
     gene            complement(1678..1950)
                     /locus_tag="PK742_12825"
     CDS             complement(1678..1950)
                     /locus_tag="PK742_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77520.1"
                     /translation="MFKYTPNNLKKLESLLGEAGYIIRYEKGRFVSGYCILENRKVIV
                     VNKYFEVESRINSLLEIMGQLPLEEEQLSDAARDFLRNNHLQSVRV"
     gene            2012..>2406
                     /locus_tag="PK742_12830"
     CDS             2012..>2406
                     /locus_tag="PK742_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU77521.1"
                     /translation="MERGILFFTVLVLGHFLTFGQIQHDPKDDEFHVFDAGIVGGINF
                     TQVHGDNLAGFNKIGMNAGGIAHINFRPGWSVSFEVLYSQKGSATYPDPNDQSAFEYK
                     LQLDYAEVPVLINYLDHNRLIAFAGLSYG"
BASE COUNT          565 a          596 c          629 g          616 t
ORIGIN      
        1 agcctgcact tctgcatcgg cggtcatcag gtaggaacca caggcatgta ccttgttggt
       61 cctgtaatac acggcaccgg tatgggcatc taccagggta taatagttgc ccggcaggtt
      121 ctcgaaatca gttgtgtgca catccacttc atagaccagt ttataggcat agccactgcc
      181 tactgtttct gatgaggtgg cttctgttac cggcaccggc aggatcttca gaaccggcag
      241 tgcggtgctg ctttccacag ttacatccag gccggaagtg gcgaaattgg ctgctgcctc
      301 cggagtcaat acaggcacag tattcagttc catggccgga aatacatcca gcccgaacat
      361 gatgaccttg ccggtagcgg ggtccatgcg cagtgtggcg ttgctgaaca atacatccag
      421 gccttcgtat tgctggcgga aggacacata gtcatacttg gaagtattga ccgcattcag
      481 tgccagttgt acgccctgca tgccgaatcc gttcagttca gtctgcagaa aatgcatggc
      541 ccgttcttcc ggtgtggtac cggtggtggc aatgccctga ccagttgccc ggtgcggcat
      601 agcagaacgt tcgttgaagg tgacccacca gttgccgtgg gcggctgtga aatcctgcca
      661 gttgtccagg ttgcgcaggg cttcctgcac tgcggggcga gggtcgttgt taccgtgaat
      721 aaaagtcacg gtattgccat caaccgggta ttgtgccgac tgcgccgaca ccatacctgc
      781 caggctgcag gccatgatca tcaaggaaaa tcgcttcatg ctgtaaagta tgagatttgg
      841 ttaacgttaa tcgagatacc tgaggagtat ttcttattca aaattacgtt tttggcttgt
      901 cacccgcaag tcagaatcct gcagcctcca gacggaggcc atcgtatgcc aggtgtacat
      961 tttccggtag tccggcctcc acagaggcat gggttcccat ctggtgagaa atatgggtga
     1021 tataggctga cccggggcgt aattcacgga tcagctccag ggcttcaggc agggagaagt
     1081 ggctgatatg cggtgtcata cgcagggcgt taatgaccag gatgtcaaga tcacggagcc
     1141 ggttcatggc tgcttcatct atcctgttgg catcggtgat ataagccagc ttcccgatcc
     1201 ggaatccaaa gaccggcaga tccagatgca tcaccctgat gggctccagg tgaatggctc
     1261 cgatgtcaaa tggttttccg gtcagccgat gcagactcac ctgcggaata ccgggatagg
     1321 tgttggttcc gaatacataa tggtattcct tgcgcagtgc ttcttcagtc agttcatcta
     1381 catacagagg aatggccttc cgttcaaagt aattaaatgg ccgcacgtca tccatgcccg
     1441 cagtgtggtc tttatggccg tgggtaaaca caatggcatc caggcggtgt acatcttccc
     1501 gcagcatctg ttgtctgaaa tccgggccgc agtcaatgac caccctggtt tcaccttctt
     1561 ccagcatgac cgacgagcgg agccttttat cccgggcatc ttcagagcgg caaacctgac
     1621 aactgcaccc gatcatgggt acaccgccgg aagtgcctgt tccgagaaag gtgagtttca
     1681 caccctcaca ctttgcaaat ggttgttgcg aaggaaatcg cgggcagcat cagaaagttg
     1741 ttcttcttca agcggaagct gacccatgat ctccagcagg ctgttgatgc ggctttccac
     1801 ttcaaagtac ttgttgacca cgatcacctt tctgttttcc aggatgcagt aacctgaaac
     1861 aaaacggcct ttttcataac ggatgatgta gcctgcttcc cccagcaatg actccagttt
     1921 cttcaggttg tttggcgtat atttgaacat aaattgctgt tgacccaaaa ataggggatg
     1981 gcttgtgaac agcaatacaa gtaatcaaca tatggaaagg ggaatacttt tttttaccgt
     2041 tctggtattg ggtcactttc tgacatttgg tcagatccag cacgacccta aagatgatga
     2101 atttcatgtt ttcgatgcag gtattgtagg aggcatcaat ttcacccagg tgcacggtga
     2161 taacctggcc ggtttcaata aaataggtat gaatgccggt ggtatagccc acatcaactt
     2221 ccggccgggc tggtctgttt cttttgaggt gctttacagt cagaaaggaa gtgcaaccta
     2281 tcctgacccg aatgatcaga gtgcatttga atacaagctg cagcttgatt atgcagaagt
     2341 gcctgtactg atcaactacc tggatcataa caggctgatc gcatttgccg gattatctta
     2401 tggaag
//
LOCUS       DAOMAX010000361         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10112_length_2402_cov_3.022156, whole genome shotgun sequence.
ACCESSION   DAOMAX010000361 DAOMAX010000000
VERSION     DAOMAX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10112_length_2402_cov_3.022156"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..2181)
                     /locus_tag="PK742_12835"
     CDS             complement(247..2181)
                     /locus_tag="PK742_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77522.1"
                     /translation="MIRRLALLQLFLACSFAVSAQLVGGEAFLHGVFVEVGVNDCGAY
                     GSSGGAPAGYHPNIGSNIGFVADSDMDGWGSGSPVYCGDYFLPGAPEEGFAVQVGANV
                     WYNSSQNCFPDEIPGSVTGYDFSAGVYSATWEGNIASENLDITTVTSLPENALYFLTT
                     VTFCNTGATPITEVYYMRNVDPDQDLVYGGTFMTTNEIVSNPPEGCDAVVTSVGGGTP
                     HCFMAMGARTPNARASYGCFNTSDGEPATVYAGGGGMGFFCSPDYEIEVGSSTFCDCA
                     TQMTLYVPEIAPGGCEEVKFVYILDLDFLDEALDYASSYEVLADDVNITDVSTVTTCQ
                     GDTILFEILGGDAYTWEWFPPTFLDTDEGTSVISIPLDTVIYYVTGYADCDTIYDTIT
                     VNAYTVEGIADAGPDAYVCPGDTVYLAGSGGNTYLWQPPTYLEDPTDPNTGVNAPPTD
                     MYYFLIAYNELGCADTDVVFIDLLPEPDIDAGQDYTIVLGGFTQLDGDGGITYLWSPE
                     ETLSNPNIENPIANPQDTTTYYLIGWDEFGCEGYDSVTINVIDPVYIVTPNAFTPNED
                     NLNDQFIPLIIGPGTLLDYQVYNRWGELVFDWQGNGPGWDGTVNGVEAELGTYIFVAR
                     AKDDLTGKELADRGTIILMR"
BASE COUNT          678 a          606 c          533 g          585 t
ORIGIN      
        1 agcgttactg ccataggcac cgcaagtatt tacacctatt tccacatagg ttccctgcat
       61 ttaggcattg gcacctacta tctgggcctg caatgcaagg ggaagacaga gcagcagcac
      121 tgtcactaat ctaatgaaat gagtgtgttt catttttatc tcagcctatt tggcattaaa
      181 aataccaaaa aactgagtaa ccgtatatcc acatagcggc atgtcattaa aatgacacag
      241 ccgaaatcat cgcatcagga tgatcgtgcc tctgtcagcc agttccttac cggtcaggtc
      301 atcctttgct cttgcaacaa aaatgtaggt tccgagttct gcttctactc cattcacggt
      361 accatcccag ccgggtccgt tgccctgcca gtcaaacacg agctcgcccc agcggttata
      421 cacctgataa tccagcaagg tacccggacc aatgatcaat ggaatgaact ggtcattcag
      481 attgtcttcg ttaggagtaa atgcatttgg ggtgacgata tagacaggat caatgacatt
      541 aatggtcaca gagtcataac cttcgcagcc aaactcatcc caacctatga ggtagtaggt
      601 tgtggtatcc tgtggattgg cgatcgggtt ttcaatgttt ggattggaga gggtttcttc
      661 cggcgaccac agataggtaa tgcctccgtc gccgtccagt tgtgtaaagc cccccagcac
      721 gatggtgtaa tcctgtccgg catcaatgtc gggttcaggc agaagatcaa taaagaccac
      781 atccgtatct gcacacccca gttcattgta ggcgatcaga aagtagtaca tatctgtagg
      841 cggagcattt acaccggtat tcgggtcggt aggatcttcc agataggtag gcggttgcca
      901 caggtaggta ttaccgccgc tacctgcaag ataaacggta tctcccggac aaacatatgc
      961 atcggggccc gcatcagcta ttccttctac agtataggca ttgacagtaa tggtatcata
     1021 gattgtatca caatcggcat aaccagttac gtagtagatc acggtatcca gggggatgct
     1081 gatcacagag gtaccttcat ctgtatccag aaaggtaggc gggaaccact cccaggtata
     1141 ggcatctcca cccaggatct caaacaaaat ggtatcgccc tgacaggtgg tcacagtaga
     1201 aacgtcggta atgtttacat catcagccag gacctcatag ctgctggcat aatcaagggc
     1261 ttcatccaga aaatcaagat caagaatata gacaaactta acctcttcgc aaccgccggg
     1321 agcaatttca ggcacatata aggtcatttg ggtagcgcaa tcgcagaatg tactggaacc
     1381 cacttcgatc tcataatcag gcgagcaaaa gaaacccata cctccaccgc cagcataaac
     1441 ggtagccggt tcaccatcag aagtgttaaa acaaccatag ctggctctgg cattgggagt
     1501 tctagcaccc atggccataa agcaatgcgg ggtaccacca cccactgagg ttacaactgc
     1561 atcgcagcct tcggggggat tggaaacgat ctcattggtg gtcatgaagg tacctccgta
     1621 caccagatcc tggtcaggat ccacatttct catataatag acctctgtta tcggggtagc
     1681 ccctgtatta cagaaagtta ctgtggtaag aaaatagagc gcattttccg gcagggaggt
     1741 cactgtagtt atatccaggt tttctgaagc gatattgccc tcccaggtag ctgagtacac
     1801 gccggcagaa aagtcataac cggttactga acccggaatt tcatcaggaa agcaattctg
     1861 gctgctgtta taccagacat ttgctccaac ctgcactgca aagccttctt caggtgcacc
     1921 tggtaaaaaa tagtcaccac aataaacagg agaaccgctt ccccacccgt ccatatctga
     1981 atcggctaca aaaccaatat tactgccaat attcggatgg taccctgcgg gagcaccccc
     2041 agatgatcca taagcgccac agtcattcac cccgacctct acaaagactc catgtaaaaa
     2101 ggcttctcca ccaaccaatt gggcagatac tgcaaaggag caggccagaa acaattgtaa
     2161 cagggctaac cggcgaatca tagtcttgat ttttggtcac taaaattatc taaataaaga
     2221 tacctcacac aattaacagg aaataaaaaa aagcgctgtc cgggttgtga acagcgcttt
     2281 ttttaggtca tttttttgtc ttatctcacc agggtgaaat atcctgagta ctccctttgt
     2341 tcatccaggg aggttctggc tctggcatat accacatatg agcccagctc ggcttctttt
     2401 cc
//
LOCUS       DAOMAX010000362         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10401_length_2365_cov_3.088312, whole genome shotgun sequence.
ACCESSION   DAOMAX010000362 DAOMAX010000000
VERSION     DAOMAX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10401_length_2365_cov_3.088312"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="PK742_12840"
     CDS             <1..1854
                     /locus_tag="PK742_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77523.1"
                     /translation="SGETLDLNGVLIDMGKLYRFVILHEFADGIAYQLSEYSEPSRLS
                     MLAAITSVNDGLDACEPPYLQASFVSATEESFVEAYRLFLSKAGSAPSLDELLALPAE
                     YYVEILPDGSFEHQAAFSPGSLDTDGDELLPGSYSLILVTMSSVDTIPTGLHQMDGII
                     YGGEAATLSEFVATSNSQEIDPFNITYTVPVDDELDWIQEIRIYIQEYGDDVLTDDEV
                     LDLESGNYIVANIASGEWSGFYPDSIPYTFNGDCIEYSKSYQFVSASVPAVDCISMQQ
                     TVYGPVEHTYEFPVATLSATDIGGTGNTNDINISIADWSGNGEVASYHVFLTRTSDAI
                     IPDFSIAKTLTTEQLTSSPDHEFNLAANQLDYLGEKTYPGTSYDVHLLIDRELPFCST
                     LASTGETVIYNPDFPDLLNLYTSVTTDGEIAMKGSFIPLEDEQYMDAYRVYIVPVENE
                     DDLEFIEFNVSAASLLSDDRYKEISPSGASLHDFFWPLEKLDTKGNEVDPVNGHYRMT
                     IMPFSSEQETQYLTYFSPMYTYDPVVSASFIGQPELQFISTDNGVMVTMSEINKDELS
                     VFDMNGRSINIVPIQTAPDQVLIPATQLPVGIYILYVKRNDLFWVKKIIVN"
     gene            2011..>2365
                     /locus_tag="PK742_12845"
     CDS             2011..>2365
                     /locus_tag="PK742_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77524.1"
                     /translation="MEVMPEINYKAYSLHNFRSIPEMKLLSEEELFDIEVVGHVLPFK
                     VNNYVIDELIDWTDYRHDAMFRLTFPQKDMLSKTHFDKMAAAMRKTSDKQELKAVANE
                     IRLELNPHPAGQMELN"
BASE COUNT          663 a          537 c          517 g          648 t
ORIGIN      
        1 tccggtgaga cattagatct taatggtgtc ttgatcgaca tgggaaaatt ataccgtttt
       61 gtcattctgc acgaatttgc tgatggaatt gcctaccagc tgtcagaata ttcggaacca
      121 tcacgtctgt ccatgttagc agccattact tctgtaaatg atggtctgga tgcctgtgag
      181 ccaccctatc ttcaagccag ctttgtatca gctacagaag aatcttttgt tgaagcatat
      241 cgcctattcc tgtcgaaagc tggcagtgca ccttcactgg atgaacttct tgcgctgcct
      301 gccgaatact atgtggagat cttaccagat ggatcatttg aacaccaggc ggcatttagt
      361 cctggaagcc ttgataccga tggggatgaa ctcctgcccg gatcgtattc tttgatactt
      421 gtaaccatgt cctctgttga caccattccg acaggtctcc atcaaatgga tggcatcatc
      481 tatggcggag aggccgccac cttgtcggaa ttcgtggcta cttcaaattc gcaggagatc
      541 gatccgttca acatcacgta taccgtacct gttgatgatg agttggattg gatacaagag
      601 atacgtatct atattcagga atatggcgat gatgtgctta ccgatgatga agtgctggat
      661 ctagaatccg gaaactacat agtagccaat attgcaagcg gtgaatggtc agggttttac
      721 ccggacagca ttccgtacac atttaacggc gactgtattg aatattcgaa gagttatcag
      781 ttcgtttccg catctgttcc tgcagtagat tgtatcagca tgcagcaaac tgtttacggg
      841 ccggttgaac atacctacga atttcctgtc gcaacacttt ctgcaacaga cattggaggt
      901 acagggaata caaatgatat caatatcagc attgcagact ggagtggaaa cggtgaggtt
      961 gcttcatatc atgtttttct gacaaggacc agcgatgcca tcatacctga tttcagcata
     1021 gctaaaacat tgacaacaga acagcttact tcttcgcctg accacgaatt caaccttgcg
     1081 gcaaaccaac tggattatct gggtgaaaag acataccctg gcacatcgta tgatgtacac
     1141 ctgttgattg acagagaatt gcctttttgc agtacgctgg catcaactgg tgaaacagtt
     1201 atttacaacc cggacttccc agatctcctg aatctgtaca catcagttac aacagatggt
     1261 gaaattgcca tgaaaggaag tttcatacct cttgaagatg agcagtatat ggatgcatac
     1321 cgcgtatata ttgttcctgt tgaaaatgaa gatgatctcg aattcattga attcaatgta
     1381 tcggctgcat ccttattgag tgacgacaga tataaagaaa tctcaccgtc tggtgcatcc
     1441 ttacatgact ttttctggcc actggaaaag ctggatacaa aaggaaatga ggtcgatccg
     1501 gtgaacggtc attacagaat gaccatcatg ccatttagtt ctgaacagga aactcaatac
     1561 ctgacctatt tcagtccgat gtatacctat gaccctgtag tatcagcatc attcattgga
     1621 caacctgaac tacaattcat cagtacagac aacggtgtca tggtaacgat gagtgaaata
     1681 aataaggatg aattatctgt tttcgacatg aacggcagat cgatcaacat tgtgcctata
     1741 caaacagcac ctgatcaggt gctcattcca gcaacccagc ttccggttgg aatatatatt
     1801 ctgtacgtaa agcgcaacga tctgttctgg gtgaagaaga taatagtaaa ttaatattca
     1861 cctatcttat tgcatatagt gccattgcaa atctggcctg tttttcgtac atttgcttgc
     1921 cgctgaaacc gggccgttct tactcgcacc tgcgatacat acactttcga gcccaacagc
     1981 tgccccattg ttttacttaa actgttgcgt atggaagtga tgcccgagat caactacaaa
     2041 gcctattccc tgcataattt caggtccatt cctgaaatga aactgctttc ggaagaagaa
     2101 ttattcgaca tcgaagtggt aggacatgtg ctgcccttca aggttaacaa ctacgtcatc
     2161 gatgaactca tcgactggac cgattaccgc catgatgcca tgttccgcct caccttcccg
     2221 cagaaggata tgctgagcaa aacgcatttt gataagatgg ctgctgccat gcggaaaaca
     2281 tccgacaagc aggagctcaa ggcggtggcc aatgagatcc gactggagct gaacccgcac
     2341 ccggcaggac agatggaact taatg
//
LOCUS       DAOMAX010000363         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10486_length_2353_cov_3.897302, whole genome shotgun sequence.
ACCESSION   DAOMAX010000363 DAOMAX010000000
VERSION     DAOMAX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10486_length_2353_cov_3.897302"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PK742_12850"
     CDS             complement(<1..573)
                     /locus_tag="PK742_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQU77525.1"
                     /translation="MKKILSSVLLSVLLAGTTPALFAGNGTEEFNVDGIQVILKSTPK
                     DVISVRLFVEGGTANYPDELQGIENLTLNLMIDGGTQNMDKTTFKTSAEKLGTEFSAG
                     TNLDYGSVNMTCIKAFWDESWNLFTDAILKPAFSENEFNLLQQQLIAGAKNAESDPDS
                     HLALTSRINAFPKSNYRKDPSGTPEALEKIT"
     gene            complement(592..1962)
                     /locus_tag="PK742_12855"
     CDS             complement(592..1962)
                     /locus_tag="PK742_12855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQU77526.1"
                     /translation="MRKIYLAFAALASMFTATAQMDLPSNFYMQKLDNGLEVLVIEDN
                     TVPLATVEIVVRNGSYTEDSSFNGLSHLYEHMFFKANKDIPSQEDFLKRVNELGIVFN
                     GTTSDERVNYFFTLSNRNLKEGLLFMNSAIRYPLFLEQEMKNENPVVDGEFQRGESNP
                     YFAMFQQFGREMWGENYYRKNPIGLHDVILTATPEKMNIIKEKYYYPNNSMLVVAGDV
                     DHNNVFPMVKEIYSSWEPCDFDPFQKWPIPEMKPLEGETMFLTESPNAQVPLILIGFH
                     GPDTRNDLQSTYAADVFSYILQQASSKLQQELVETGLAFQVQVGYQTAKYTGPIQIIL
                     VPNPMKVQEAYNKLWEQISMWSDPDYFTDEQLETAKRLLAIDDAYGKESTSSFVHTVT
                     YWWASATIGYYTNYVDNLQKVTREDIDRYIKTYISGKPHVVGLALNPAMKDMIDLKGM
                     GFVKAE"
     gene            complement(2031..>2353)
                     /locus_tag="PK742_12860"
     CDS             complement(2031..>2353)
                     /locus_tag="PK742_12860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU77527.1"
                     /translation="LHTGTFDEQLPELLNKPSIVFDLVYIDGNHRLEPTLRYVDMITP
                     YLSEKSVVVLDDIHWSKEMHQAWQILQSNQSFCYSVDLFYKGLLFREPGLQVHQSASF
                     CIPH"
BASE COUNT          594 a          528 c          571 g          660 t
ORIGIN      
        1 agtgattttt tccagcgctt ccggtgtacc ggaggggtct ttacgataat tggactttgg
       61 aaaagcattg atgcgggagg tcagtgcgag gtgtgagtcc gggtcgcttt cagcattttt
      121 ggcccctgca atcagttgct gttgtaacaa attgaattca ttttctgaaa aggcgggctt
      181 caggatggca tcggtgaaca ggttccatga ttcatcccaa aaagccttga tacaggtcat
      241 gttcacagat ccgtaatcca gattggtgcc ggcactgaat tcagtcccca gcttttctgc
      301 agatgtcttg aacgtggtct tgtccatatt ttgggtgcca ccatcaatca tcaggttcag
      361 agtcaggttc tcaataccct gcaactcatc aggataattg gcggttccac cttccacaaa
      421 gaggcgaaca gagatcacat ctttgggtgt tgatttcaaa atgacctgta tgccatccac
      481 attgaactct tcagttccat taccggcaaa cagggcaggg gttgttcctg caagcagcac
      541 tgacaggagt acactagata atatcttttt cattgtgcgg gtatttattg gttattcagc
      601 tttaacaaat cccattcctt tcagatcgat catatccttc atggcagggt tcagggcaag
      661 acctacaaca tgaggtttgc cactgatata ggtcttgata tatctgtcaa tatcttcacg
      721 ggtcaccttc tgcaggttat ctacatagtt ggtatagtag ccaatggtgg cactggccca
      781 ccagtaggta acggtatgaa caaagcttga agtggattct ttaccatagg catcatcaat
      841 agccagcagg cgcttggcgg tttccagttg ttcatcagta aagtaatcag ggtcgctcca
      901 catgctgatc tgttcccaca atttgttgta ggcttcctgc actttcattg ggttgggtac
      961 caggatgatc tggataggtc cggtgtattt ggcagtctgg tagcccacct gaacctggaa
     1021 ggccagtcct gtttctacca gttcctgctg caatttgctg gatgcctgct gcagaatgta
     1081 ggagaacaca tcagcagcgt aggttgactg caggtcgttt cgggtgtcag gtccatggaa
     1141 tccaataagg atcaggggta cctgagcatt ggggctttca gtgaggaaca tggtttcacc
     1201 ttccagtggt ttcatttcag gtatcggcca tttctggaag ggatcaaaat cacacggctc
     1261 ccagctgctg tagatctctt ttaccatagg gaagacattg ttgtgatcca catcacctgc
     1321 aactaccagc atggagttgt tcgggtagta gtatttctcc ttgatgatgt tcatcttttc
     1381 aggagtggct gtcaggatca catcatgcag accgataggg ttcttgcggt agtagttctc
     1441 gccccacatc tcccggccaa actgctggaa catggcaaag taagggttgg attctccacg
     1501 ctggaattca ccgtccacca cagggttctc atttttcatt tcctgttcca ggaacaacgg
     1561 atagcggatg gcggaattca tgaacagcaa accttcttta agattccggt tgctgagggt
     1621 aaagaagtaa ttgacacgtt cgtctgaggt tgtaccatta aatacgatgc ccaattcatt
     1681 gaccctcttc aggaaatctt cctgagaagg tatgtctttg ttggctttga agaacatgtg
     1741 ttcgtacagg tgcgacaaac cgttaaagga actgtcttca gtgtaggaac cattgcgcac
     1801 aacgatttct acagtagcaa gtggcacagt gttgtcttca atcaccagca cttcgaggcc
     1861 gttatccagt ttctgcatat agaaattgga aggcaggtcc atttgcgccg tggctgtaaa
     1921 catactggcc agggctgcaa acgcaagata tatttttctc atatgaaggg tttacgaagc
     1981 aaagatacct aacagacagt aagcaggcca gaagctgccg ttaaacaatg ttaatgcgga
     2041 atgcagaaac tggcagactg atgaacctgt aacccgggtt cacggaacaa tagtcctttg
     2101 tagaacagat ctactgaata acagaacgac tggttgcttt ggaggatctg ccatgcctga
     2161 tgcatctcct tgctccagtg aatgtcatcc agaacgacca cacttttttc agacaggtat
     2221 ggcgtgatca tatctacata ccgcaaggta ggttccagcc ggtgattgcc atcaatatat
     2281 accaggtcaa aaacaattga aggcttgttc agcagttcag gtaattgctc atcaaaggtg
     2341 cctgtatgca ggc
//
LOCUS       DAOMAX010000364         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10569_length_2344_cov_4.354303, whole genome shotgun sequence.
ACCESSION   DAOMAX010000364 DAOMAX010000000
VERSION     DAOMAX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10569_length_2344_cov_4.354303"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PK742_12865"
     CDS             complement(<1..1175)
                     /locus_tag="PK742_12865"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HQU77528.1"
                     /translation="MRYLIILSAGLFLLGCKADNPLTVQTERGKLLSCELLQEYSIAE
                     ADSIITGYDEFLAIYPVDYPIRIYRITYITVDPFGEETTASGAVILPMDTTVSFPLCS
                     YQHGTITERYEVPSFEGGELFLGIVFAPGGYVVCMPDYLGLGLGEGLHPYCHAKSEAT
                     ATVDMLRATRQVCDEQDIHLNDQTFLFGYSQGGHATMAAARELELYHTDEFTLTASAP
                     MSGPYDISGAQTELVVSDEPYSAPYYLPYLMFAYNEVYDMYDQYSDFLKAPYDTLLPP
                     LFDGQHAGGEIDGVMPDVPKEIIRPEVLDDFLNNSSNPFRIALADNDLIYDWVPQAPM
                     ILFYCSGDELVTSQNSVVAKEVFDAAGATSVSLWETNPTLGHEGCAEPSFIYCRGWF"
     gene            1364..>2344
                     /locus_tag="PK742_12870"
     CDS             1364..>2344
                     /locus_tag="PK742_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQU77529.1"
                     /translation="MNLSNYTIKSQEAIQAAQSLAMQMDHQQMTNEHLLKALLSLDEE
                     VISFLLKKLNVNLSHLQSRLDAQLEALPGSGAGQGGQYLSREMNMSLAKAATFLKEFN
                     DEFISVEHLLLGILVSGDGVSKLLKEQGVNEKDLKAAIKDLRKGSTVNTASAEGQYNA
                     LEKYAINLNERAGSGKLDPVIGRDEEIRRVLHILSRRTKNNPILVGDPGVGKTAIAEG
                     IAHRIVNGDVPENLKSKTIFSLDMGALIAGAKYKGEFEERLKAVIKEVVQSEGQIILF
                     IDEIHTLVGAGGGEGAMDAANILKPALARGELRSIGATTLNEYQKYFEKDK"
BASE COUNT          631 a          546 c          599 g          568 t
ORIGIN      
        1 tcaaaccatc cccggcaata gatgaaactg ggttctgcac atccttcatg gcccagcgta
       61 gggttggttt cccagagtga tacggaagtt gcgcctgcag catcgaatac ttctttagct
      121 accacgctgt tctgactggt taccagttcg tcgccggagc aatagaacag gatcatagga
      181 gcctgtggta cccagtcgta gatcaggtca ttatctgcaa gagctatccg gaatggattg
      241 gatgaattgt tcagaaaatc gtccagcact tccggcctga tgatctcttt aggaacatcc
      301 ggcatgaccc cgtcaatttc tccaccagca tgttgcccgt caaacaaagg aggcaacagt
      361 gtgtcgtaag gtgctttcag aaaatcgctg tattgatcat acatgtcata cacctcattg
      421 tatgcaaaca tcagataggg caggtaatag ggtgcggaat agggctcgtc actcaccacc
      481 agttctgtct gggcgccgct gatgtcatac ggcccactca tgggtgcgga agcggtcagg
      541 gtgaattcat ctgtgtggta taactccagt tcgcgggcag cggccatggt ggcatgtccg
      601 ccttgtgaat agccgaataa gaaggtctgg tcgttcaggt gaatgtcttg ttcatcacaa
      661 acctgtctgg tggcccggag catatctacc gtagcagttg cttcgctttt ggcatggcaa
      721 tagggatgca gaccttcgcc cagaccgaga cccaggtaat caggcataca taccacataa
      781 cctccgggtg caaatacgat gcccagaaat aactcacctc cttcaaagga aggcacttcg
      841 taccgctcgg tgatggtgcc atgctggtaa gaacaaagcg gaaaagatac agtggtatcc
      901 atcggcagaa tgacagcacc tgaggctgtg gtctcttctc cgaaaggatc caccgtgatg
      961 taagtgatgc gatatattcg gatgggataa tccaccggat aaatagccag gaattcatcg
     1021 tatccggtaa tgatagagtc tgcttctgct atactgtatt cttgcagtaa ttcgcagctt
     1081 aataatttac ctcgttctgt ctgaaccgtc agcggattgt cggccttaca accaagtaaa
     1141 aaaagtcctg cggatagtat gatcaggtat cgcataggta gttggttgga ttaaaagtag
     1201 gcactttacc tgactcatgc tgttgttcat ccggtccttt tggcagatta tattcttttt
     1261 acatgccatt ttgatggtac tccaccagtg taatcagaca gcttgtcatt gttgtagatc
     1321 aggaatcttt tttgatgcgg gcatagcagg caaatcaacg atcatgaact tatcgaatta
     1381 taccattaaa tcacaagagg ccatccaggc tgcgcagtcg ctggccatgc agatggacca
     1441 ccagcagatg accaatgagc acctgcttaa agctctgttg tctctggatg aagaggtcat
     1501 ttccttcctg ttgaaaaaac tgaatgtgaa cctttcgcat ctgcagtcca gactggatgc
     1561 gcaactggaa gctctgcccg gatctggcgc aggtcagggt ggtcagtacc tctctcgtga
     1621 aatgaatatg tcgctggcca aagcagccac attcctgaaa gaattcaatg atgaatttat
     1681 ctcggtggaa cacctcctgt tgggcattct ggtttccgga gatggtgtga gtaaactatt
     1741 gaaggaacaa ggtgtgaatg aaaaggacct gaaagcagcc attaaagatc tgcgcaaagg
     1801 ttcaactgtg aataccgcat cggcagaagg acagtacaat gcccttgaaa aatatgccat
     1861 caacctgaac gaaagagcag gctccggaaa actggatccg gtaatcggac gtgatgaaga
     1921 gatcagacgg gtgctgcata tcctctcacg gcgcaccaag aacaatccta ttctggtggg
     1981 tgatccgggt gtgggtaaga ccgccatcgc cgaaggcata gcccaccgta tcgtgaacgg
     2041 tgatgtaccc gagaacctga aaagcaagac catcttttcc ctcgacatgg gagctctcat
     2101 tgccggcgcc aaatacaagg gcgaatttga agaaagactg aaagcggtga tcaaggaagt
     2161 ggtacaaagt gaaggccaga tcatactctt tatagacgag atacataccc ttgtgggtgc
     2221 cggaggcggt gaaggagcca tggatgcagc caatatcctc aaaccggcac tggcccgtgg
     2281 agagctgcgc agtattggtg ccaccaccct gaatgagtat cagaagtatt ttgaaaagga
     2341 taaa
//
LOCUS       DAOMAX010000365         2344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10570_length_2344_cov_4.147663, whole genome shotgun sequence.
ACCESSION   DAOMAX010000365 DAOMAX010000000
VERSION     DAOMAX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10570_length_2344_cov_4.147663"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /gene="malQ"
                     /locus_tag="PK742_12875"
     CDS             complement(<1..1348)
                     /gene="malQ"
                     /locus_tag="PK742_12875"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQU77530.1"
                     /translation="MISERASGILLHPTSLPGKHGIGSLGKAAFGFVDFLVQAKQRYW
                     QILPLGPTGYGDSPYQCFSSKAGNPYLIDLDLLAEQGLLEADELPVSGAFGMTKVDYG
                     KVIDMKLNLLAKAEKRFREQGNTEIKERYHQFVEINRYWLDPYALFMALKEKFGKKPW
                     YEWPHDYMMHQESVLGPAREELAAAIDFHRYLQYEFFNQWMAVKEYANNKGVLIIGDI
                     PIYVAMDSADTWSNTGLFQFDEQKKPIAVGGVPPDYFSKTGQLWGNPLFNWEPMIHDD
                     FGWWRDRIRTSLVLFDLVRIDHFRGFAGYWAVPYGEETAINGSWKDAPGMALFDSIRR
                     HLGDLPIIAEDLGVITPDVAALRDNFQLPGMKILQFAFDSAEENDFIPHTYHKNCLVY
                     TGSHDNDTSKGWFKSAKAEDKQYCLDYCKGTQRTIHWDLMRTAWASVANTAIAPMQD"
     gene            2004..>2344
                     /locus_tag="PK742_12880"
     CDS             2004..>2344
                     /locus_tag="PK742_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77531.1"
                     /translation="MNIRAIVFVAFATSISSLLGQVPTGPDIAWQQCYGGFGDDYFTD
                     AIITQDNGILAAIRPLSDDGDLEGMTKEEASGWVIKYAPDMTIQWQKHFGYPDCFRTI
                     YKLSELVNGDI"
BASE COUNT          585 a          576 c          579 g          604 t
ORIGIN      
        1 catcctgcat cggtgcgatg gccgtgttag ccacagatgc ccaggctgtg cgcatcagat
       61 cccagtggat ggtgcgctgg gtgcctttgc aataatcgag gcagtattgt ttgtcttccg
      121 ccttggctga tttgaaccaa cccttagaag tatcattgtc gtgcgaaccg gtatagacca
      181 ggcagttctt gtggtaggta tgcggaatga aatcgttctc ttctgccgag tcaaatgcga
      241 actgcaggat cttcataccc ggtagctgga aattatcgcg gagagcagcc acatcgggtg
      301 tgatgacacc caggtcttcg gcgatgatgg ggaggtcgcc caaatgcctg cggatgctgt
      361 cgaacaaagc catacccggt gcatccttcc agctgccgtt gatggcggtc tcttcgccgt
      421 agggaactgc ccagtagccg gcaaagcccc ggaagtggtc gatgcgcacc aggtcgaaca
      481 ggaccaggct cgtgcggatc ctgtcacgcc accagccgaa atcatcgtgg atcatcggct
      541 cccagttgaa gagcggattg ccccaaagct gaccggtctt gctgaaataa tcgggcggta
      601 caccacctac tgcgatgggt tttttctgtt cgtcgaactg gaacagtccg gtgttggacc
      661 aggtatcggc actgtccatc gctacataga taggaatatc tcctataatt aaaacacctt
      721 tgttgttggc gtactccttc accgccatcc actggttgaa gaactcatac tgcaggtagc
      781 ggtggaaatc gatggcagcg gccagctctt cacgggcggg acctaatacc gactcctggt
      841 gcatcatgta gtcatgcggc cattcgtacc agggcttttt gccaaacttt tccttcaatg
      901 ccatgaacag cgcatacgga tccagccagt agcggttgat ctctacgaac tggtggtagc
      961 gttctttgat ttctgtattg ccctgttccc ggaaacgctt ctctgctttc gccagcaggt
     1021 tcagtttcat gtcgatcacc tttccgtagt ccaccttcgt cataccgaag gcaccgctca
     1081 ctggcagttc atcggcttcc agcaaccctt gttccgccag caggtccaga tctatgagat
     1141 agggattgcc cgctttggag ctgaagcact ggtagggact gtcgccgtaa cccgtgggcc
     1201 ccaggggcag tatctgccag taccgctgct tcgcctgtac cagaaaatcc acaaaaccga
     1261 atgctgcttt tcccagagaa ccgatgccgt gtttgcccgg cagagaggtg ggatgtaata
     1321 agatgccgct ggccctttca gagatcataa cacaagatta attcaggata aatctggtgt
     1381 aaagataggg gagataaagg cggaatgcgg aggacggaaa tcgaaattcg aaaaacggaa
     1441 ttcggaacag gggatagtat ttcttttccg gacagagtac tctccatcca ctcccacacc
     1501 cactcccaca ctcactccaa cccccgcacc cgctccccat tagccgtccc gccggtgtta
     1561 ccgggtgtgg atgaacaatt cagtgtttga atcacatcac agaggaacgc cggcgttttt
     1621 aaataggagg ctattcggat agatgaacca cagagttcct ttctctcctg cggcgggtaa
     1681 acccttaact ctgtggggac aggaaaccct atgtggacgg aagaatcaac aacaaaattt
     1741 taaacttcag cttttacctg cttcaaattc cagtaaatgt aatttttact gccgtttggt
     1801 gaaagtgcca tattttccct caaattggcg aacacaagac tagtctaaca ggatgaacgg
     1861 cttagagaat ggccttcctg ctccacaaac aaagatggac cacatctggg acatcactag
     1921 attgtaaaaa atctaattga tgtgtttcct tttgaggaaa gtcaccattt tgttaagcta
     1981 aataattaac ttttgcatta gttatgaata taagggccat agtttttgtt gcatttgcta
     2041 catcaatttc ttctctatta ggtcaagtgc ctacaggccc tgatattgcc tggcaacagt
     2101 gttatggagg ttttggtgac gattatttta cagatgccat cattacacaa gacaatggta
     2161 ttctggcagc cattaggcct ctatctgatg atggtgatct ggaaggaatg accaaagaag
     2221 aggcttctgg ttgggtgata aaatatgcac cggatatgac catacagtgg cagaagcact
     2281 ttggttatcc ggattgcttt agaaccattt ataaattaag tgaattagta aatggagata
     2341 taat
//
LOCUS       DAOMAX010000366         2343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10574_length_2343_cov_3.968531, whole genome shotgun sequence.
ACCESSION   DAOMAX010000366 DAOMAX010000000
VERSION     DAOMAX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10574_length_2343_cov_3.968531"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PK742_12885"
     CDS             complement(<1..799)
                     /locus_tag="PK742_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQU77532.1"
                     /translation="MKVFKFGGASVCDADSIRNVGRVLQLFAPEKLVIVVSAMGKTTN
                     AIEQMAGKAYAGLPWEAYMQQLAESHLQTAHELNLDKQTIERLIANLRSNLVNYKDLP
                     FDQYYDQTVPFGELLSSALLSAWLQANGQPTTLLAAGDLLVTDKTWREATVDMEASIM
                     AIQKQVIPALANGHVLTQGFIGKYSSAFYTTLGREGSDYTGAILAHALDAEGLWIWKD
                     VQGVLTGDPKEFSDMSLLEELSYYEAIEMTYYGATVIHPKTIQPLRMK"
     gene            complement(821..1267)
                     /locus_tag="PK742_12890"
     CDS             complement(821..1267)
                     /locus_tag="PK742_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU77533.1"
                     /translation="MQIHIRKAVIEDMAFVHRLVQELAEYEKEPEAVTTTVAQYEADL
                     ETGRFSVLVAETGSGVIAGMALYYPVYSTWKGPYLWLEDLVVQESFRRHGIGTLLMDA
                     LENEARAAGFPLLRWQVLDWNTPAISFYKARGAQHDAAWHTYRQWL"
     gene            1327..>2343
                     /gene="fbp"
                     /locus_tag="PK742_12895"
     CDS             1327..>2343
                     /gene="fbp"
                     /locus_tag="PK742_12895"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQU77534.1"
                     /translation="MKVKTLVEFIIERQSDFPYATGELTRLLSDISIAAKVVNREVNK
                     AGLVDILGAEGSENIQGEEQQKLDVFANTQFIEALNAGGEVCGIASEELDDFIGLDGK
                     VSRNGKYVVLIDPLDGSSNIDVNVSIGTIFSIYRRISFTGSATLEDFLQKGHEQVASG
                     YVIYGSSTMLVYTTGHGVNGFTLDPSIGEFCLSHANITIPESGNIYSINEGNYVHFPD
                     GVKKYIKYCQEADSPTNRPYTSRYIGSLVADFHRNMLKGGIYIYPETAKSPKGKLRLL
                     YECNPLAFIAEQAGGKASNGFKRIMEVDPKELHQRCPLYIGSRLMVEDVEKFLEASRE
                     SAGLA"
BASE COUNT          569 a          573 c          609 g          592 t
ORIGIN      
        1 gtttcatgcg cagcggctgg atggtcttcg gatgaatgac cgttgctccg taatacgtca
       61 tttctatggc ctcataatag ctgagttctt ccagcaggct catatctgaa aactctttcg
      121 gatcgccggt tagcacacct tgcacatcct tccatatcca caaaccttca gcatccaggg
      181 catgggcaag aatggcacct gtataatcag atccttcccg gccaagggtg gtatagaatg
      241 cgctggagta tttgcctata aagccctgag tgagcacatg tccgtttgcc agagcgggga
      301 tgacctgctt ttgaatggcc atgatgctgg cctccatatc taccgtggct tcccgccagg
      361 tcttgtcggt caccagcaga tcgcctgcgg ccaggagggt ggtaggttgt ccgttcgcct
      421 ggagccaggc cgataacaag gcagatgaca gcagttcgcc gaagggcacg gtctggtcat
      481 aatactgatc gaaagggagg tctttgtaat taacgaggtt cgatcggagg ttggcgataa
      541 gccgctctat cgtctgtttg tccaggttca gttcatgagc agtttgcagg tggctttctg
      601 caagctgctg catgtaggcc tcccagggca gtcctgcata agctttaccc gccatctgct
      661 caatggcatt ggtggtcttt cccatagcag aaaccacgat gaccagcttt tccggggcaa
      721 atagctgcag cacacgaccc acattcctga tgctgtctgc atcacagact gaggcccctc
      781 cgaatttgaa tactttcaca gcctgcaaaa ctacaacaga tcaaagccat tgacgatacg
      841 tatgccaggc ggcatcgtgt tgtgcgccac gagctttgta gaagctgatg gccggcgtgt
      901 tccagtccag cacctgccag cgaagcagcg gaaagccggc tgctctggct tcgttttcca
      961 gtgcatccat cagcagggtg ccaataccat gccggcggaa agattcctgc accaccaggt
     1021 cttccagcca gagatagggc cccttccagg tagagtaaac aggatagtac aaagccatac
     1081 cggctatgac tcccgatccg gtttcagcta ccagcacaga gaatctgcct gtttccaggt
     1141 ctgcctcata ttgtgcaacg gtggtggtaa cggcctccgg ttctttttca tattctgcca
     1201 gttcctgaac cagacggtgc acaaaggcca tatcttcaat aactgctttt ctgatgtgta
     1261 tctgcatgcc gctaaagata aagaggccga aacggtggaa ttactacctt tgcccacaac
     1321 acagttatga aggtaaagac cctggtagag ttcattatag agcggcagag tgattttccc
     1381 tatgcgaccg gagagttgac ccgtttgtta tctgacatca gtatcgctgc caaagtggtg
     1441 aaccgggagg tgaacaaagc aggtcttgtg gacattctgg gtgccgaagg aagtgagaat
     1501 attcagggcg aagaacagca gaaactggat gtgtttgcca atacccagtt cattgaagcc
     1561 ctgaatgcag ggggagaggt ttgtggtatc gcttctgaag aactggacga cttcattggt
     1621 ctggatggta aggtttcacg caacggtaaa tatgtggtgc tgatcgatcc gctggacgga
     1681 agctccaata tagatgtgaa cgtatctatt ggtaccattt ttagtatata cagacgaatc
     1741 tcctttacgg gaagtgccac cctggaagat tttctgcaga agggtcatga acaggtagcc
     1801 agtggttatg tcatctatgg ctcatccact atgctggtct ataccaccgg acatggggtg
     1861 aatggcttta cactggatcc ctccattggt gagttctgtt tgtcgcatgc caatatcacc
     1921 atccctgaat cgggaaatat ctactccatc aacgaaggga actatgtgca ttttccggat
     1981 ggggtaaaga agtatatcaa gtattgccag gaagctgatt ctcctaccaa caggccttat
     2041 acttccaggt atatcggttc tctggtggca gactttcatc gcaacatgct caagggtggt
     2101 atttatatct accctgaaac cgccaaatct cctaaaggaa aactccgttt actgtatgaa
     2161 tgcaatccgc tcgcattcat tgctgagcag gcaggaggga aggcttccaa cggattcaag
     2221 cgcatcatgg aagtagatcc taaagagctg catcagcgat gcccgttgta tataggttcc
     2281 aggttgatgg tggaagatgt ggaaaagttt ctggaagcca gccgtgagtc tgccggcctt
     2341 gct
//
LOCUS       DAOMAX010000367         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10619_length_2338_cov_4.584319, whole genome shotgun sequence.
ACCESSION   DAOMAX010000367 DAOMAX010000000
VERSION     DAOMAX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10619_length_2338_cov_4.584319"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..2090)
                     /locus_tag="PK742_12900"
     CDS             complement(3..2090)
                     /locus_tag="PK742_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77535.1"
                     /translation="MLRPVLCCLLACNLSATIAQVSFDSRIVDIGNIGLRVTNVGTLG
                     RPDVRNTPTGDPSMEYPLNTGIEHLFEAGFWIGAEVDGQILVSTSAKDAASGYSTGGS
                     GFEFTAEVGNTIAQRSSLTNSEFFSFDAISHEDMLMDFSDQNIIIPGTAITINEHTQP
                     LNAQVHLESYAWNFSFADYFVILRYTITNSGTSTWENVRTGLWSDMVVRNVNVNTDFG
                     SAFFSHGGYGFEDSLLANYAFDVDGDPGFTNSYGSLQFLGMEWRDQFIHPNNAEVVIA
                     AGYPEPEVHSNFWIFNSTGTPPYNAPANDVERYEKMGSSLNYSDPDLVDFLQDPNTSG
                     GLTNLISAGTLESVEPGESFEMVWAVVAAKQIETGGTTGPEMDTEEGRAQLLEHLGWA
                     KRTYLGEDLNENGLLDDGEDLDDDGILDRYILPEPPATPKIKVIAGDKTIDIYWDEQA
                     EFSIDPISKEVDFEGYRLYRTQPGDDFNLDMLGDANQIAQWDLPGNAIGYNNGLSSIR
                     LAQPEVIEGDTFYYHYQLDQVLNGWQYLIVLTSFDRGDEALNIGSLESSFVDGSVRIW
                     PGSLPSSDVQVPVGVYPNPYPLHAAWDGSTSTTRKIVFYNLPARALITVFTASGEIVA
                     ELQHDGDYNGSDAGWFDAYGGDPEDRVFSGGEHAWDILSQSKQTISQGIYLFSVKDLD
                     TGEIRQGKLVVID"
BASE COUNT          568 a          602 c          592 g          576 t
ORIGIN      
        1 tactaatcta tgaccaccag ctttccctgt ctgatctcac ccgtatccag atctttgacg
       61 ctgaacaggt agatgccctg agagatggtt tgtttgcttt gagagaggat gtcccaggcg
      121 tgttcgccac cactgaagac acggtcttcc gggtctccgc catacgcatc gaaccagccg
      181 gcatcactgc cattgtagtc gccgtcgtgc tgcagctctg ccacgatctc gccgctggcc
      241 gtgaacactg tgatcagtgc cctggcaggc agattgtaaa aaacgatctt cctggtagtg
      301 gaagtggagc catcccaggc ggcatgcaac gggtaagggt tcggatacac accaacaggc
      361 acctgcacat ctgaggaagg taaggatccc ggccagatgc gcacagatcc atctacaaaa
      421 gaagattcca gcgaaccgat gttcagcgct tcatcaccgc gatcgaagga ggtgagtaca
      481 atgagatact gccagccgtt cagcacctga tccagctggt agtgataata gaaagtatca
      541 ccttctatga cttccggctg ggcaagtctg atggaagaca atccgttgtt atagccgatg
      601 gcattgccgg gaaggtccca ctgtgctatc tggttggcat cgccaagcat gtcgagattg
      661 aagtcatcgc caggctgggt gcggtacagc cgatagcctt cgaagtccac ttctttcgag
      721 atcggatcga tggagaattc ggcttgttca tcccagtata tatcaatggt tttgtcgccg
      781 gctatcactt tgatcttcgg agtggcagga ggctccggta aaatatatct gtccaggata
      841 ccatcgtcgt ccaggtcttc accatcatcc agcaagccat tctcattcag gtcttcgccc
      901 agataggtgc gcttcgccca gcccaggtgt tccaggagtt gcgcacgtcc ttcttcggta
      961 tccatctccg gtccggtagt gccacctgtt tctatctgct tggcagctac tacggcccat
     1021 accatttcaa aactttcgcc cggctctacg ctttccaggg tgcctgcaga aatgaggttg
     1081 gtaagtccgc cgctggtatt cgggtcctgc agaaaatcca ccagatccgg atcggaatag
     1141 ttcaggctgc ttcccatctt ttcatagcgt tccacatcat tggcaggtgc gttgtaaggt
     1201 ggtgtaccgg tcgagttgaa tatccagaaa ttcgaatgca cttctggttc agggtatccc
     1261 gctgctatga ccacctccgc attgttgggg tggatgaact gatcacgcca ttccatacca
     1321 agaaattgca gagagccgta tgaattggta aagcccggat caccatctac gtcaaatgca
     1381 tagttggcca gtaaactgtc ttcaaagcca tagccaccat ggctgaaaaa ggcagaacca
     1441 aaatcggtat ttacgttcac attgcgcacc accatatcgc tccacagacc ggtgcgtaca
     1501 ttctcccagg tggatgttcc gctgttggta atggtatagc gaaggatgac aaagtaatct
     1561 gcaaatgaaa aattccaggc ataagattcc aggtgcacct gtgcattcaa aggctgcgta
     1621 tgctcgttga tggtgatggc cgtaccgggg atgatgatat tctggtctga aaagtccatc
     1681 agcatatctt catggctgat ggcatcaaag gaaaagaatt cgctgttggt aaggctggac
     1741 cgctgggcga tggtattgcc cacttctgcg gtgaattcaa aacccgatcc gccggtcgaa
     1801 tatccgctgg cagcatcttt ggctgaagta gataccagaa tttgtccgtc cacttcggca
     1861 ccgatccaga aacccgcttc gaacaggtgt tctataccgg tattcaaagg gtactccata
     1921 gacggatcgc cggttggggt attgcgcaca tcaggcctcc ccagggttcc tacattggtg
     1981 acccgcagcc cgatattacc aatgtcaacg atcctggaat caaaagacac ctgtgctatg
     2041 gtagcagata agttgcaggc gagcaggcag cataataccg ggcgaagcat gatgcgaaat
     2101 tagaagaaag aagggattct gcaccggcaa acagcagaag tgaatgttaa tacaaccggt
     2161 atttcgttcc ggaatttcct gctatttctg tcttgtttca gcctttgaca gcatcactta
     2221 tcagtcaata agatgcagtt atcagataat caggtcctgt ttggtcagga atggggaatt
     2281 tcgaaaagct gccatgcaca cacattcggc cgctctgcta tccctgcctg aaggtctg
//
LOCUS       DAOMAX010000368         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10634_length_2336_cov_4.217887, whole genome shotgun sequence.
ACCESSION   DAOMAX010000368 DAOMAX010000000
VERSION     DAOMAX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10634_length_2336_cov_4.217887"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="guaA"
                     /locus_tag="PK742_12905"
     CDS             complement(<1..598)
                     /gene="guaA"
                     /locus_tag="PK742_12905"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQU77536.1"
                     /translation="MTEKILIVDFGSQYTQLIARRVRELNVYSEIVPCNKLPEITDDI
                     KGIILSGSPFSVSSPQLPDFDYDHVANKRPLLAVCFGAQYIAQHHGGKVAPSSKREYG
                     RAFLHLVEKECPLFYGVNDESQVWMSHSDTVMAIPDGFHITAKTASIDVAAYSSDEGA
                     TPFPIHCIQFHPEVTHTSEGLTMLENFVVRISGCEQSWT"
     gene            1263..2297
                     /locus_tag="PK742_12910"
     CDS             1263..2297
                     /locus_tag="PK742_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQU77537.1"
                     /translation="MKKYWFFVVGSLCMTLMVFPSWKASAPQAFTLSSKVNDVLFALG
                     QPKPSWYPEDLSEAAIERGREMIFNGRTTAPDGSKSKFISVYYMCTNCHNTVIEDPIL
                     TVADPQARLEYAAQHDIPFLQGTTFYGITNRETWYNDDYYKKYGDLVKPANENLAEAV
                     QLCAMECSQGRPVEDWEIQSIIAYYTTIGYSLSDIGFSEQDLSALNADIASGKNTVKW
                     VENIRGRYLHKSPATFLEPPYDNYWETLRGKGDAANGKLVYELSCQACHKSYGVSDVV
                     FGDDKQEFKQFNRWFKKNDWWLFQVVRHGTHPAPGHREYMPHYTKERMTDQQVEDLRA
                     YILERAGKGS"
BASE COUNT          638 a          515 c          611 g          572 t
ORIGIN      
        1 gggtccagct ttgttcacaa ccggaaatgc gcacaacaaa attctccagc atggtcagcc
       61 cttcgctggt atgggtcact tccgggtgaa actggatgca atggatgggg aagggtgtgg
      121 caccttcatc actgctgtag gctgccacat caatagaagc cgttttggca gtaatgtgaa
      181 acccgtcagg gatggccatg acggtatcgc tgtggctcat ccatacctgg ctttcatcgt
      241 ttacgccata gaacagcgga cattcttttt ccaccaggtg cagaaatgcc cttccatatt
      301 cccttttgga agagggggcc actttgcctc catgatgttg cgctatgtac tgggcgccaa
      361 agcatactgc cagcagcggg cgtttattag ccacatggtc gtagtcgaaa tcgggtagtt
      421 ggggggaaga gactgaaaac ggactaccgg acaggatgat gcctttgata tcgtctgtga
      481 tctccggcag cttgttacag ggcacgatct cggagtacac attcagttct ctgacacgcc
      541 tggcaatgag ctgggtgtac tgtgaaccga agtcaacgat gagaattttt tcagtcatgc
      601 gcaaagatag aaaacgacag cagaacgctg attgctcagc atgtctataa tggtcttatc
      661 gggtggggaa agtgtggaag gctgttcccc aaagtgggga aaaattccac accccaatgt
      721 tggtaccggt gccggatggt gagcagtttt aattacctga tgcctggaaa atgctcgtag
      781 tatgatcggc agccgaaagg aatatgggct gcaggctttg ttgggagggg tttttgggaa
      841 agatcaggag aaaaaaaaag cagaggttac cactttcggg cgccctctgc tatccccatg
      901 aaaacacaca caatatctat tgttgcaaac ggagtgccaa caccaaaagt tccgcagtat
      961 ttcaaaatgg aacagagatc acacagggga caacttgtgg tttctcttta caactgaggg
     1021 aaggagttac acagagtaag acatacaact cttgtataac cctgtggtag ctaacaaaaa
     1081 aaagcagagt tcaccgttac cggatccctc tgctatcccc atgaaaacac aataaagtaa
     1141 gtgtgtagtg tctaaagaac tgatgtaaaa ttagtattaa ttccagaaaa acaaatctgc
     1201 atgtgctgat ttttcttttt tcagcaataa aagataaact ttgtttgttc aatacacgaa
     1261 agatgaaaaa gtactggttt tttgtagtgg gatctttgtg tatgaccttg atggttttcc
     1321 cttcatggaa agcttcagcc ccccaggcat tcaccctgtc atccaaagtc aatgacgtcc
     1381 tgttcgcact tggtcaaccc aaaccttcct ggtatccgga agacctgtct gaggctgcca
     1441 tagagcgggg acgtgaaatg atcttcaatg gtcgcaccac agcacctgat ggcagcaagt
     1501 cgaagttcat cagtgtgtac tatatgtgca ccaactgcca caacaccgtg atagaagacc
     1561 cgatcctgac tgtggctgat ccgcaggcaa gattggaata tgccgcacag catgatattc
     1621 ctttcctgca gggaacgacc ttttatggca tcaccaaccg cgagacctgg tacaacgacg
     1681 attactacaa gaagtacggt gatctggtga aacccgccaa tgagaacctt gctgaagcag
     1741 tgcagctctg tgccatggag tgctctcagg ggcgtccggt ggaggactgg gaaattcagt
     1801 ccatcattgc atactatacc actataggat attcactttc tgatatcggt ttttctgagc
     1861 aggatctgtc agctttaaat gctgatattg cttcaggaaa gaatactgtg aagtgggtgg
     1921 aaaatatccg cggtcgttat ctgcataaat cgcctgccac cttcctggag cctccttatg
     1981 ataattactg ggaaacactt cgagggaaag gtgatgctgc caacggaaag ctggtctatg
     2041 aacttagttg tcaggcctgt cataagagct atggggtgag tgatgttgta ttcggtgatg
     2101 acaaacagga attcaaacag ttcaatcgct ggttcaaaaa gaacgactgg tggttgttcc
     2161 aggtggttcg ccacggcaca catccggcac ccggacaccg ggagtatatg ccacattaca
     2221 ccaaagagcg catgaccgat cagcaggtgg aagacctccg ggcgtatata ctggaaagag
     2281 cgggaaaggg gagctgaggg cagtgaggag tgaggagtga ggagtgagga gtgagg
//
LOCUS       DAOMAX010000369         2316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10797_length_2316_cov_3.599735, whole genome shotgun sequence.
ACCESSION   DAOMAX010000369 DAOMAX010000000
VERSION     DAOMAX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10797_length_2316_cov_3.599735"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PK742_12915"
     CDS             complement(<1..489)
                     /locus_tag="PK742_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HQU77538.1"
                     /translation="MEIVNKVAQSGLLTIDLEAYFPADKVCGFDLKSFLFRELILKEK
                     DFREAMAAIDWSAYSGKILAIHCTADAIIPQWAYMLVTVYAAPYAEKIYLADPDQALH
                     KYYEEIVHDFDTTPYEGQRIVVKGCSKHPVPADAYVALTRKLRPVAKSIMYGEPCSTV
                     PIY"
     gene            complement(528..>2316)
                     /locus_tag="PK742_12920"
     CDS             complement(528..>2316)
                     /locus_tag="PK742_12920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQU77539.1"
                     /translation="HPYIKNYYVGLSGSGKYDPAAFNQYITFIKNPENQQNNPQVLEA
                     YFDFLMRESLAIKEYTNNKYTNLFVKADFVPKWMVDRDYSVKNTRVNFDFVDIPYTSV
                     SDSSIAVSDADLKAYYNENKNRFKQKQASRVIEYIVFPFYATSEDSAAILAKLQEDMT
                     NLASAKNDSAFIAVRSEDPERITRAFQNRNYFYSSLIDSSVVDSLFNAPVGSLVGPYL
                     KNDQYFTSSLIKDRTLMPDSADVRHILISNQVREDAAAKSLADSLLNAIRSGSDFAEL
                     AKQFSDDTGSGAQGGDLGWSTPQTDYVKPFKDFVFRTGQTGQPTVVESMFGYHIIEIK
                     ELRGRSEYLLAYHLSRFIEASSATADSVDLKANEFFNTYKTPESFETGAAEMGLQYRT
                     SPPFASNQFEIPGIPESRSIVTWAYTAKEGEFNYFNTYTDRIVVAYIKNARDKGIADL
                     ADVEEEVRVNVIREKKGEQLGKQLADAMQGGKDLNAIAASVNSSVKTSTNASFGTPYA
                     PGIGLEQKVVGIVMATGQDATTGVIEGNRGVYVAKITGITAAPAEGETTMNRNQLTYG
                     VRNKMSQQNIIRSLVEKADVEDNRYMFGY"
BASE COUNT          568 a          552 c          538 g          658 t
ORIGIN      
        1 ataaatagga acggtgctgc atggttcgcc gtacatgatg cttttggcta caggcctcag
       61 cttgcgtgtg agtgctacat aagcatctgc aggtaccgga tgcttgctgc atcctttcac
      121 aacaatacgt tgcccctcat agggtgtggt gtcaaagtcg tgaacgatct cttcataata
      181 tttatgcagg gcctgatcgg gatcggccag gtagatcttt tctgcgtagg gtgccgcata
      241 cacagtcacc agcatataag cccattgcgg gatgatggca tctgccgtac aatgtatggc
      301 caggatctta ccggaatacg ctgaccagtc tatggctgcc atcgcttcac gaaagtcctt
      361 ttccttcaga ataagttcgc ggaaaagaaa tgatttcagg tcaaagccac ataccttgtc
      421 tgccggaaag taggcttcca ggtcaatagt gagtaggccg ctctgggcga ctttattgac
      481 gatctccatg taccggttaa acggttaacc gttctaaaaa gtttggctta atagccgaac
      541 atgtatcggt tgtcttccac atctgccttt tcaacaagac tgcggatgat gttctgctgg
      601 ctcattttgt tacgcacgcc ataggtcagc tgattcctgt tcatggtggt ttcaccttcg
      661 gccggagcag cggttatgcc ggttatcttc gccacatata cacctctgtt accttcgatc
      721 actccagtgg tagcgtcctg accggtggcc attacgatac ccaccacctt ttgctccaga
      781 ccgatgccgg gtgcataagg agtgccaaaa ctggcattgg tagaggtctt tacagaactg
      841 ttcacacttg ctgctatcgc attgaggtct ttacctccct gcatggcatc agccagttgc
      901 ttgcccaact gctcaccttt cttttcgcga atgacattca cacgaacctc ttcctctaca
      961 tctgccaggt cagcgatacc cttgtcgcgt gcattcttga tataggctac cacaatgcgg
     1021 tctgtatagg tgttgaagta attgaattca ccttctttgg cagtataagc ccaagtcacg
     1081 atactacgtg attccggaat accgggaatt tcaaactgat tggatgcaaa aggcgggctg
     1141 gtgcgatact gcaggcccat ttctgcagca cccgtttcaa aagactcggg tgttttatat
     1201 gtattgaaga actcattggc cttcagatct acactatctg ccgttgctga acttgcttca
     1261 atgaatcggc tcagatggta agcgagcagg tattcactgc gaccacgaag ttctttgatc
     1321 tcaatgatgt ggtaaccgaa catggactct acaacggtgg gttgtccggt ctgaccggtg
     1381 cggaaaacga aatccttgaa gggttttaca taatcggtct gcggggtgct ccatccaagg
     1441 tcaccaccct gagcaccgct tcctgtatca tctgagaact gttttgctaa ttctgcaaaa
     1501 tcactgccac tgcgaatagc attcagcaaa ctgtcagcca gggatttggc tgctgcgtct
     1561 tcacgcacct ggttgctgat cagtatgtgg cgaacatcag cagaatcagg catcagggtc
     1621 cggtctttga tcaaactgct ggtaaagtac tgatcgtttt tcagatacgg ccccacaaga
     1681 cttccaacag gtgcattgaa caggctgtca accacagatg aatctatcag actgctgtaa
     1741 aaataattgc ggttctgaaa ggctcgtgtt atgcgttctg gatcttcaga acgaacagca
     1801 ataaatgctg agtcattctt ggcagaagcc agattggtca tgtcttcctg cagtttggcc
     1861 agtatagcag cactgtcttc agaggttgca taaaaaggga aaactatgta ttcaattaca
     1921 cggctggcct gcttctgctt gaatctattc ttgttctcgt tgtagtatgc cttcaggtct
     1981 gcatcagaaa ctgcaatgct ggaatcagat acactggtat agggtatatc tacaaaatca
     2041 aagttcacac gggtgttctt gacagaataa tcacgatcta ccatccactt gggtacaaag
     2101 tctgccttta caaacagatt ggtatattta ttatttgtgt actctttgat agccaggctc
     2161 tcacgcatca gaaaatcaaa ataagcttcc agtacctgag ggttgttctg ctgattttcc
     2221 ggatttttaa tgaaggtgat atactggttg aaggcagcag gatcatattt tccactgccg
     2281 ctcagtccaa catagtagtt cttgatatat gggtgc
//
LOCUS       DAOMAX010000370         2310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_10850_length_2310_cov_4.924612, whole genome shotgun sequence.
ACCESSION   DAOMAX010000370 DAOMAX010000000
VERSION     DAOMAX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10850_length_2310_cov_4.924612"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PK742_12925"
     CDS             <1..1835
                     /locus_tag="PK742_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77540.1"
                     /translation="IFSFTFVSAAGCDSIVTVDLTVNTVSSTTEDVSICDGGSYTLPD
                     GTIATTDGTYISTLTSAFGCDSIITTNIIVTDMYLISLSPVICEGDLYVLPDGSVTDL
                     AGSWSFTFTSVVGCDSMVVVDLEVNPVYENILPVSLCEGESIVLPDGTLVDDPVSGIY
                     TYTFISAAGCDSIVGVDLVVDPVDSVVLSPAICSGESYTLPDGSTTSSEGDYFYVFTA
                     DSGCDSLVTVNLVVVESWDMLIDTTICSGDSLLLPDATWASTDGTYVFDLMSSGGCDS
                     VVTINLLLEMLPELSISTVDDAFCLDAGIQEIAVSPAGGVLSGAGIAGGFFDPELAGS
                     GGPFWITYSYSTATGCLSSDSISVWVNDLPVVSFDAPPFICLEADPLLLSGDPAGGSF
                     YGDGMADGYFVPSLSDTNVAAIYYTYTDAAGCTGTWTESISILTNVVDAGPDQVIEQG
                     DSTMLLASGTGSLSWSPNGSLSCVDCSHPVASPQTTTTYTLTSTDPNGCIAVDQVTVT
                     VIPSEEYTVFVPNTFTPNGDGANDLFLLYGYNLDLVLSFRIYDRWGQIVYLDEQFDPY
                     DNQRGWDGSFQGQPVNAGVYAWVAEVRTKAGKQVIQYGNVTLLR"
     gene            complement(1844..>2310)
                     /locus_tag="PK742_12930"
     CDS             complement(1844..>2310)
                     /locus_tag="PK742_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77541.1"
                     /translation="TTICLGESLDIFADGALFYNWFPETAVPCTDCSSMSISPTENVS
                     LSVTGTNAVGCTSTQSFTVTVEVCQSVADNWPANWNLYPNPAADHITLETGSAAGLKQ
                     LILMDANGRNVRNWSVTGQTTQLSLSGLPAGNYLLIIQEDDHRFSNNIIKYQ"
BASE COUNT          528 a          510 c          588 g          684 t
ORIGIN      
        1 ggattttctc attcacgttt gtttcagcag caggctgcga tagtatcgta acggtagatc
       61 taaccgtcaa cacagtttca tcgaccactg aagacgtcag catctgcgac ggcggctcct
      121 acacattacc ggatggcacc atcgccacga ccgatggcac ttatatttct actttgacat
      181 ctgcattcgg ttgtgatagc atcatcacca ccaatatcat tgtgacagat atgtatctta
      241 tctccctgtc gcctgtcata tgtgagggtg atctgtatgt cttgcccgat ggctctgtaa
      301 ccgatttggc cggtagctgg tcttttacat tcacctctgt tgttggttgc gatagtatgg
      361 ttgtggttga tctggaagta aatccggttt atgagaatat tctgccagta agtttgtgtg
      421 aaggcgagag tattgtattg cctgatggta cactggttga tgatccggta agcggaattt
      481 atacctatac ttttatatca gcagcaggct gcgacagtat agtaggtgtt gatcttgttg
      541 tagatccagt agatagtgtg gtgctttctc cggctatttg cagcggagaa tcttacaccc
      601 tgccggatgg cagcacgacc tcttccgaag gtgattattt ttatgtgttt acggcagatt
      661 cgggctgtga cagcttggta acagtaaacc tcgtggtagt tgaaagctgg gatatgctga
      721 tagataccac tatctgttcc ggcgacagtc tcctgttgcc agatgctacc tgggcctcca
      781 cagacggcac ctatgtattt gatctgatgt cgagcggtgg atgtgattca gttgttacca
      841 tcaacctgtt gctggagatg cttccagaac ttagcatttc aaccgttgat gatgccttct
      901 gcctggatgc aggaatacag gaaattgcag tttcaccggc aggtggtgtc ctttctggag
      961 caggaatagc gggtggattt tttgatcctg agcttgcagg ttcaggaggt ccgttctgga
     1021 tcacatacag ttattcaact gcaaccggtt gtctgtcttc tgatagcata agtgtctggg
     1081 tcaacgacct gcctgttgta tcatttgatg ctccgccttt tatatgtctg gaggctgatc
     1141 ctctgttgtt atctggtgat cccgcaggtg gtagctttta tggtgacggc atggcagatg
     1201 gctactttgt accttctttg tctgatacca atgtagctgc catttactac acctatacag
     1261 atgcagcggg ttgcacggga acctggactg aaagcatttc cattctgacg aatgttgttg
     1321 acgcaggtcc tgatcaggtc attgaacaag gagattccac catgttgctg gcatccggaa
     1381 cggggtcact cagctggagc cctaatggct cgcttagctg tgttgattgc agtcatcctg
     1441 tggccagtcc gcaaacaact actacctaca cgcttacatc aacagatcca aatggctgta
     1501 ttgctgttga tcaggtaacg gtgactgtca ttccatcaga agagtacacg gtatttgtac
     1561 ccaatacctt tacacctaat ggcgacggag ctaatgatct tttcctgtta tatggatata
     1621 acctggatct ggtattgtct ttcaggatct atgaccgctg gggtcagatc gtttatctgg
     1681 atgaacaatt cgatccatat gataatcagc ggggctggga tggaagcttc caggggcaac
     1741 ccgtcaatgc aggggtatat gcctgggtag cagaagtacg caccaaagcc ggaaagcagg
     1801 tgatccagta tgggaatgtt accctgcttc ggtagtattt attttattga tatttgatga
     1861 tgttgttgct gaaacgatga tcatcttcct gaatgatcag cagataattt cctgccggca
     1921 agcccgaaag agaaagttgt gttgtttgtc cggtaactga ccagttgcgc acgttcctgc
     1981 cgtttgcatc catcagtatc aactgtttta gtccggcagc acttccggtt tccagggtga
     2041 tatggtctgc agcaggattg ggatatagat tccagtttgc aggccagttg tcggcaacac
     2101 tttgacagac ctctacggta acagtaaaac tctgggtgct ggtacagcca acagcgttgg
     2161 ttcctgttac tgacaaggag acattttctg tcggactgat gctcatacta ctgcagtctg
     2221 tgcaaggaac agccgtttcc ggaaaccagt tgtagaacag agcgccatca gcaaaaatgt
     2281 ccaggctctc acccaggcag atggttgtgt
//
LOCUS       DAOMAX010000371         2275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11141_length_2275_cov_3.589189, whole genome shotgun sequence.
ACCESSION   DAOMAX010000371 DAOMAX010000000
VERSION     DAOMAX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_2275_cov_3.589189"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="hflX"
                     /locus_tag="PK742_12935"
     CDS             complement(<1..838)
                     /gene="hflX"
                     /locus_tag="PK742_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQU77542.1"
                     /translation="MQDLSVQDKERAILIGLVTQYQTVEQLEEYLDELAFLAQTAGAE
                     VIERFYQKLTTPEKRTYIGKGKLEEVQRYVEEKDIDLVLIDDEISPAQLRNIEEAVKV
                     KVLDRSMLILDIFASRAQTMQAKTQVELAQTQYLLPRLRGMWTHLDRIKGGIGMRGAG
                     EKEIETDRRVAQQRISLLKQKLKDIDKQSHTQRKNRGEFIRVALVGYTNVGKSTLMNV
                     LSKSEVFAENKLFATLDTTVRKVVFERTPFLLSDTVGFIRKLPHHLVESFKSTLDEVR
                     EAD"
     gene            962..>2275
                     /locus_tag="PK742_12940"
     CDS             962..>2275
                     /locus_tag="PK742_12940"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQU77543.1"
                     /translation="MSSRLLYSGVLTALLFTAGSAIAQAPALPNGYAPWELDLLDSYI
                     PYKTTSGITSPPELPVRASAEWEEIDALLVTWTGYTNTIREIVRYAQEECEVYILCAD
                     TNTVKTSLESASIPLDNITLVEGGFNSVWARDYGQWNVYANDVDSLYLIDWIYNRPRP
                     LDDAVPELMSQLTGLPIYTTTEVPYDLVHTGGNFMTDGWGTGFSSRLVLDENDGNDYS
                     LEPKTEGEIDYIMDAFMGISTYIKMETLPYDQIHHIDMHMKLLDEETILVGEYPEGEA
                     DGPQIEANIEYIQDNYTSVFGTPYKFERILMPPDEAGNFPDDGSWWNPGDYRTYTNFV
                     FINKTVIVPAYEEQYDTTAYRILSDLLPGYRIVMIDCNEIIQALGAIHCVTKEVATNE
                     PLLITHQPLQDTEELFADYEVDAWILHRDGIAEAEIFYTTDTAAGF"
BASE COUNT          534 a          627 c          539 g          575 t
ORIGIN      
        1 catctgcttc ccgcacttcg tccagggtgc tcttgaaact ttccaccagg tgatggggca
       61 gcttgcggat gaaacctacc gtgtcagata acagaaacgg agtgcgttcg aataccacct
      121 tgcgcacggt cgtatccagt gtggcgaaca atttattctc tgcgaacacc tcgcttttgc
      181 tcagcacatt catcagggta ctcttaccca cgttggtata tcccaccagg gccaccctga
      241 tgaactctcc ccggttcttg cgctgggtat gactttgttt gtcaatgtcc ttcagcttct
      301 gcttcagcag gctgatgcgc tgctgggcca ccctccggtc ggtctctatt tctttttcac
      361 ccgcacctcg cataccgata ccacccttga tcctgtcgag gtgggtccac atacctctca
      421 gtcttggcag caggtactgg gtctgcgcca gctccacctg ggtcttggcc tgcatggtct
      481 gtgctctgga agcaaaaatg tccaggatga gcatgctgcg gtccagcact ttcaccttca
      541 cggcttcttc gatattgcgc agctgagcag gactgatctc gtcatcgatc agtaccaggt
      601 cgatgtcttt ttcttccacg tatcgctgta cttcttccag ctttcccttc ccaatatagg
      661 tgcgcttttc cggagtggtc agtttctgat agaaacgctc tatcacttct gctccggccg
      721 tttgcgccag gaatgccagc tcgtccagat actcttcgag ctgctccacg gtctggtatt
      781 gggtcaccag tcctatgagt atcgctcttt ccttatcttg tactgaaagg tcctgcatcg
      841 ggattgcgaa gttagccctt tgtgttgtaa cgattccgtt tcttttgtta cactttttac
      901 tgaaagaggg aactatgttt agagtccctg tggtgcacac accctaacca acccaacgac
      961 tatgagcagc aggttacttt actccggggt gttgaccgcc ttactattta cagcaggatc
     1021 agccatcgca caggcccctg cactacctaa tggatatgcc ccctgggagc tggatctgct
     1081 tgactcctac ataccctaca agaccacttc cggcatcacc agtccgccgg aactgcctgt
     1141 tcgtgccagc gcagaatggg aagagataga cgccctgctg gtcacctgga ccggctatac
     1201 caataccatc agagagatcg tgcgatatgc acaggaagaa tgcgaggtgt atatactatg
     1261 tgcagatacc aacacggtaa agaccagcct tgaaagtgca tccataccac tcgataatat
     1321 caccctcgtg gaaggtggct tcaattctgt atgggcccgc gattacggcc agtggaatgt
     1381 ctatgccaac gatgtggatt ccctctacct catcgactgg atctataacc gtccgcgacc
     1441 gctggatgat gccgttccgg aactgatgtc gcagctgacg ggactgccta tctataccac
     1501 tacagaagtg ccttatgacc tggtgcacac cggaggaaat ttcatgaccg atggctgggg
     1561 aaccggattt tcatcccgcc tcgtcctgga cgaaaatgat ggtaacgact acagcctgga
     1621 gccaaaaaca gaaggcgaga tcgactatat catggatgca tttatgggca tcagcactta
     1681 tatcaagatg gagaccctgc cttacgatca gatccaccac attgatatgc atatgaagct
     1741 gctggatgaa gagaccatac tggtgggcga atacccggaa ggggaagctg atggtccgca
     1801 gatagaagcg aacatcgaat acatccagga caactacacc agtgtattcg gcacacctta
     1861 taagttcgag cgcatcctca tgccgcctga tgaagcaggc aattttccgg acgatggttc
     1921 ctggtggaat cccggcgatt accgcaccta caccaacttc gttttcatca acaagacagt
     1981 gatcgtaccg gcttacgaag aacaatatga tactaccgct tatcggatcc tatcagatct
     2041 gctacccggc tatcgcattg tgatgataga ttgtaatgag atcatacagg cattgggagc
     2101 catccattgc gtaacaaaag aagtggccac caacgagcca ttgctcatca cccaccagcc
     2161 tttgcaggat acagaagaac tcttcgccga ttatgaagtg gatgcctgga tactgcaccg
     2221 cgatggtatt gcagaagcag agatctttta taccacagat actgcagcag gtttc
//
LOCUS       DAOMAX010000372         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11258_length_2263_cov_4.176178, whole genome shotgun sequence.
ACCESSION   DAOMAX010000372 DAOMAX010000000
VERSION     DAOMAX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11258_length_2263_cov_4.176178"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PK742_12945"
     CDS             <1..641
                     /locus_tag="PK742_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQU77544.1"
                     /translation="EEERWDSLQAVTTRSIELFPNQALGYYFNGVSFQQTKAYDQAVT
                     MYKRSIPMSVGNNQLRAEIWLRLGDTYHEMGKHPESDQAYDESLKLDPANPYTLNNYA
                     YYLSLRGEQLDKAADMARKAVRLVPDNPSLLDTYAWVLYRSSMYKEAAQVIEDALRVG
                     GQNSAVVLEHYGDILYQLDRKEEALQYWKKAQEKGAENEKLLRKIRDEKLYE"
     gene            634..1374
                     /locus_tag="PK742_12950"
     CDS             634..1374
                     /locus_tag="PK742_12950"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HQU77545.1"
                     /translation="MNKLLFGAWLLFLLPACAGSRYTHGRIKDVSGEELYTSMQAELV
                     QCETLHGRARVNMDDDGVQSMLADITMQTDSFIGISMRVLGMEVARIYITADSVQIMD
                     RVNKQYLPRSMADFSARFGIPLSLETLQDLLLGRPVWDEGTWYPVVAEDLYRLQATRP
                     GLQNTLDIYPGFLPARQMITDTEQQRTLDLETGGYKKTDGQTFSMNRHIRLDAVDKYA
                     IQLEWASLSLNDPVSFDFQVNPRYEVVH"
     gene            1358..>2263
                     /locus_tag="PK742_12955"
     CDS             1358..>2263
                     /locus_tag="PK742_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77546.1"
                     /translation="MKWYTKILSLPLLLLPLTLSAQDKLSLQKKYDQLQSEIKDAQDL
                     LSQTREKKNNSLSQVKLLNRKINVREEMIRNLSMQVQQTAIQIRESSEKITAMEANLE
                     SMREDYARMVYYAYVNDKEYEPIHFLFASGSINDAYTEIEYVREYTTYRKQQASAIKA
                     AQAALELRIAELEKDKVTKQNLLTEEQDQRNTLSKEKEEQDVTLQGLQGEEKKILAQI
                     DQKKKDANALNKQIQAIIAEEIRKEKERAEAEARKAAEKAAKESGTSTTSTVKEEKKT
                     TAPVNTGLTPEMALIGQNFASNKGRL"
BASE COUNT          684 a          540 c          583 g          456 t
ORIGIN      
        1 ctgaagaaga acgctgggac agtctgcaag ccgttaccac ccgcagtata gagttgtttc
       61 ccaaccaggc tctgggttac tattttaatg gagtctcctt ccagcagacc aaggcctatg
      121 atcaggctgt gaccatgtac aaaaggagca ttcccatgtc tgttggcaac aaccagttgc
      181 gggcagagat atggctgcgg ctgggcgata cctatcatga aatgggaaaa catccggaaa
      241 gcgatcaggc gtatgatgaa agcctgaaac tcgatccggc caatccttat acattaaata
      301 attacgcata ttatttatca ctgagaggag agcagctgga caaggccgcc gacatggcca
      361 gaaaagctgt tcggctggtg cctgacaatc cttcactact ggacacctat gcctgggtgc
      421 tgtatcgttc atccatgtac aaggaagctg cgcaggtcat agaagatgcc ctgcgggttg
      481 gcggacaaaa cagtgctgtg gtgctggaac actatggtga tatcctgtat cagctggaca
      541 gaaaggaaga agccctgcag tactggaaga aagcacagga aaaaggcgct gaaaacgaga
      601 aactgctcag gaagatccgc gacgaaaaac tctatgaata agctgctctt tggtgcatgg
      661 ctcctctttt tgcttcctgc ttgtgccgga agcagataca cccatgggcg catcaaagac
      721 gtcagcggcg aagagctcta taccagcatg caagccgaac tggtgcagtg tgaaacgctg
      781 catggacgtg ccagagtcaa tatggatgat gatggtgtcc agtctatgct ggcagatata
      841 accatgcaga cagacagttt cataggcata tccatgcggg tgctggggat ggaagttgcc
      901 aggatataca tcaccgcaga ttctgtacag atcatggacc gggtgaacaa gcaatatctt
      961 ccccggtcga tggctgattt ttctgcaagg tttggcattc ctctttcgct tgaaacgctt
     1021 caggatctcc tgctgggaag accggtttgg gatgaaggta cctggtatcc tgtcgtggcc
     1081 gaagacctct acaggttaca ggctacccgt ccgggtctgc agaacacact ggatatctac
     1141 cccggttttc tgccggccag acaaatgatc acagatacag aacagcagag aacgctggat
     1201 ctggaaaccg gcggatacaa gaaaaccgac ggacagacgt tttccatgaa tagacacatc
     1261 cggctggatg cagtggacaa gtatgcaatt cagctcgagt gggcatctct atctttaaac
     1321 gaccctgttt cgttcgattt tcaggtaaac ccccggtatg aagtggtaca ctaaaattct
     1381 gtctcttccc ctgctgctcc tcccactgac actcagtgcc caggacaagc tcagcctgca
     1441 gaaaaaatat gaccagctgc agagtgagat caaagatgca caggacctgc ttagccagac
     1501 ccgtgaaaag aagaacaaca gcctcagtca ggtcaagttg ctcaatcgca agatcaatgt
     1561 gcgtgaagaa atgatccgca acctgagtat gcaggtgcag caaacagcta ttcagatccg
     1621 tgaatcttca gagaagatca ccgctatgga ggccaacctg gagagtatgc gtgaagacta
     1681 tgcccgcatg gtttattatg cttatgtgaa cgacaaggaa tatgaaccca tccacttttt
     1741 gtttgcctcc ggaagtatca atgatgccta tacagaaatt gaatatgtaa gagaatacac
     1801 aacgtacaga aaacagcagg catctgccat aaaagctgca caggctgcac tggagctgcg
     1861 catagctgaa ctggagaagg acaaggtgac caaacaaaat ctgctgacgg aagaacagga
     1921 tcagcgaaac accctttcca aagaaaagga agaacaggat gtcaccctcc aggggctgca
     1981 gggtgaagaa aaaaagatcc tggcccagat agaccagaag aaaaaagacg ctaatgccct
     2041 gaacaagcag atccaggcca ttattgcaga agagatcaga aaagaaaagg aacgagcgga
     2101 agcagaagcg aggaaagcgg cagaaaaagc cgccaaagaa agcggtacgt ccaccaccag
     2161 tacggtcaaa gaagaaaaga agaccacagc accggtcaat accggcctga ccccggaaat
     2221 ggccctcata ggccagaact ttgccagcaa caagggcagg tta
//
LOCUS       DAOMAX010000373         2261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11280_length_2261_cov_4.186763, whole genome shotgun sequence.
ACCESSION   DAOMAX010000373 DAOMAX010000000
VERSION     DAOMAX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11280_length_2261_cov_4.186763"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PK742_12960"
     CDS             complement(<1..305)
                     /locus_tag="PK742_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQU77547.1"
                     /translation="MDRTEQFRQTINEGHTFKGDHIVLGAAMLDGQAVADAQVKIPLA
                     TLNRHGLVAGATGTGKTKTLQVLCEQISDKGISVLIMDIKGDVSGMAMPGTANPKID"
     gene            371..1744
                     /gene="radA"
                     /locus_tag="PK742_12965"
     CDS             371..1744
                     /gene="radA"
                     /locus_tag="PK742_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQU77548.1"
                     /translation="MKTNKAKTAFFCQECGFESPKWLGRCPSCQSWNTFTEEVISRPD
                     EQEALWKDEDKRKPNTPVRLQDIQATEGKRIVTGDLELDRVLGGGIVPGSIVLIGGEP
                     GIGKSTLLLQIAMNLIGQKVLYVSGEESEQQIRMRAERIPGGNDQLYVSTESNVAKVF
                     KQVKQLKPEVLVIDSIQTLVSDFIDATAGSISQIKECAGQLQRYAKESGVPVFLIGHI
                     NKEGTIAGPKVLEHMVDTVLQFEGDRHYTHRILRAQKNRFGSASEIGIYRMETDGLRQ
                     VNNPSELLITQKDEPISGVAVAATMEGMRPMLIEVQALVSPAVYGNAQRTATGFDLRR
                     LNMLLAVLEKRGGLAMGNKDVFLNMAGGLKVDDPAIDLAVVAALISSFEDVPIPLHYC
                     FAAEVGLSGELRAVNRIEQRIMEADKLGFESIFIAKHHAGGLQKNKFRIEVQAFGKLE
                     DIYQKLF"
     gene            1744..>2261
                     /locus_tag="PK742_12970"
     CDS             1744..>2261
                     /locus_tag="PK742_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQU77549.1"
                     /translation="MVSVLKDIWTGLTDIIYPRVCPACDEVLGLEDRDICFTCRHTLP
                     ETDYHTFPGNPVAVHFWGKVPLTAATALYHFSKDGKVQHMLHHLKYKHRKDIGLLLGN
                     FLAKTLKDHPVFSTCDLIVPVPLHPKKQLQRGYNQSAVIAEGIAEKTGIPVGKDILIR
                     NTYTATQTRKGRL"
BASE COUNT          567 a          556 c          637 g          501 t
ORIGIN      
        1 tcatcgatct tcgggttggc ggtgcccggc atggccatcc cgctcacatc gcccttgata
       61 tccatgatga gcacactgat acccttatcg ctgatctgct cgcacagcac ctgcaacgtc
      121 ttggtcttgc cggtacctgt tgcacctgcc accagtccat ggcggttgag ggtagccagc
      181 ggaattttta cctgcgcatc tgctactgcc tgtccgtcca gcatggcagc tcccagtacg
      241 atgtggtcgc ctttgaatgt atggccttcg ttgatggtct gacggaattg ttcggtcctg
      301 tccatgggcg aatgacagcg caatgctgct tttttggttt atttgctgca aagataaccc
      361 ctgccgacga gtgaagacga acaaggccaa aacagcattt ttctgccagg agtgtggatt
      421 tgaatcgccc aagtggttgg gtcgatgccc gtcctgccag agctggaaca cctttacaga
      481 agaagtgatc tccagacccg atgaacagga ggcactgtgg aaggacgaag acaaacgcaa
      541 accgaatacc cctgttcgtt tgcaggacat tcaggcaacc gaaggcaaac gcatcgtgac
      601 cggcgatctc gaactggacc gtgtactggg aggcggcatc gtgccgggaa gcatcgtgct
      661 gataggcggt gaacccggaa taggcaagtc cacactgctt ctgcagatcg ctatgaacct
      721 gatcggacaa aaggtgctgt atgtaagcgg ggaagaaagt gagcagcaga tccgcatgcg
      781 tgccgagcgc attccgggtg ggaatgatca gttgtatgtc agcaccgagt cgaatgtggc
      841 gaaagtattc aaacaggtaa agcagctgaa gccggaagta ctggtcatcg attcgataca
      901 gaccttagta agtgatttca tcgatgccac cgcagggagc atctcgcaga tcaaagaatg
      961 tgcaggccag ttgcaacgct atgcgaaaga gagtggggtg cctgtttttc tgatcggaca
     1021 tatcaataag gaaggcacca tcgccggacc caaggtcctg gaacatatgg tggatacggt
     1081 attgcagttc gagggcgaca ggcactatac ccatcgcatc ctgcgtgcac agaagaatcg
     1141 cttcggctct gcttctgaga tcggcatcta caggatggaa acggacggac tcaggcaggt
     1201 gaacaacccc agcgaactgc tcatcaccca aaaagatgaa ccgatcagtg gtgtggcagt
     1261 ggctgctacc atggagggca tgcgccccat gctcatagaa gtacaggcgc tggtaagtcc
     1321 tgcggtgtat ggcaatgctc aacgcactgc tactggtttc gatctcagaa gactgaacat
     1381 gttactggcg gtactggaaa aacgcggggg gctggccatg ggaaacaagg atgtgttcct
     1441 gaacatggcc ggcggactga aagtagatga tcctgccatt gacctggcgg tggtggctgc
     1501 gctgatctcg tcgtttgaag acgtgcccat tccattgcat tactgctttg cagcggaggt
     1561 gggattgtcg ggcgaattgc gtgcagtgaa ccgcatcgaa caacgcatca tggaagccga
     1621 taaactgggc tttgaaagca tcttcatcgc caaacaccat gccggtggat tgcagaagaa
     1681 taaattcaga atagaagtac aggccttcgg caagctggag gatatctacc aaaaactgtt
     1741 ctgatggtca gcgtgctgaa agacatctgg acaggtctga cagacatcat ttaccccagg
     1801 gtctgtccgg cttgcgatga agtgctgggg ctggaagacc gcgacatctg tttcacctgt
     1861 cggcacacct taccggagac cgattatcat acttttcccg gcaatccggt agcggtgcat
     1921 ttttggggga aggtaccatt gaccgctgct actgcgctct atcatttttc gaaggacggg
     1981 aaggtgcagc atatgttgca tcatctgaaa tacaaacacc ggaaagacat cggattattg
     2041 ctgggaaatt tcctggcgaa aacgctgaag gatcatccgg ttttcagtac ctgcgacctg
     2101 atcgttcctg ttcccctgca tccgaaaaaa caattgcagc ggggatataa tcagagtgca
     2161 gtcattgcgg agggcattgc agaaaagacc ggtatcccgg tggggaaaga tatactgata
     2221 cgcaatactt acacagctac ccagaccaga aaaggcaggc t
//
LOCUS       DAOMAX010000374         2260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11289_length_2260_cov_4.312018, whole genome shotgun sequence.
ACCESSION   DAOMAX010000374 DAOMAX010000000
VERSION     DAOMAX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_2260_cov_4.312018"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1729)
                     /locus_tag="PK742_12975"
     CDS             complement(209..1729)
                     /locus_tag="PK742_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQU77550.1"
                     /translation="MKRIIVPVLGVFLAGTVFTSCADRSAVTGFKYNDTKWGGFENID
                     YRGQATGPNLVLIQGGTFTMGMTEQDVTYEFHNVPRRVTVSSFYMDETEVSNLHYREY
                     LYWTTRTFGIDFPEIIDNALPDTLVWREELAYNEPYVEYYFRYPSYDEYPVVGISWLQ
                     ANEFCRWRSDRVNEAIMADKGYMDININSYNEDNFTTQSYYAGVYTTGVKKGVKDYSP
                     EAGDSKLGRTIKMEDGILLPDYRLPTEAEWEYASLGLIGNQVEGNEMLTDRRLYPWDG
                     NTMREPSSKERWSQGKMLANYKRGRGDYAGLAGKLNDNAIVTANIYSYQPNDYGLYNM
                     AGNVNEWVFDVYRPLTYYDMEDLNPFRGNKYMTKELDEDGNIAELDSMGRVRYRPQTD
                     EEVVNRRNYKKGDVKNYEDGDTASLAYYDYGHSTLVSDRARVYKGGSWADEAFWLSPG
                     SRRYLEEDQSTATIGFRCAMIRLGGDVDNYTRSGNIFKSTDRQKKINEKYRKNTRR"
     gene            complement(1781..>2260)
                     /locus_tag="PK742_12980"
     CDS             complement(1781..>2260)
                     /locus_tag="PK742_12980"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQU77551.1"
                     /translation="AAFVTEKAYTGISFLHVNQPPNTFYDYEEDRLPMAISWQGGLFL
                     KQKHRKRDPFMAEPTVQLISQGGFSQLQLGSYFAKSFVMGGFFLKNNFSGMSDVSILA
                     GLKKDWILFTYSYDVTVGGLSGETGGSHEVGLIFRLDQAGKHSKGKYYSVLPRPSVF"
BASE COUNT          535 a          580 c          568 g          577 t
ORIGIN      
        1 catccagggc tttttgcgca aacttgttgc catctgcctg ttcgcctttc agcgctacga
       61 acagattacc gggtttcact ttccggctgt ctatttcgac accggaacac cggctgtgga
      121 tctccagtag ttgtgttaca gacatggcct caaaaataaa aaagcccgcc attgaaaggg
      181 cgggctttta tgttcatttc aagtcgtttt accgacgtgt attctttctg tatttctcgt
      241 tgatcttctt ctggcggtcg gtgctcttga agatgttacc acttctggtg tagttgtcca
      301 cgtcgccacc caggcggatc atagcacaac ggaaaccgat ggtagcagtt gactggtctt
      361 cttcgaggta acgacggcta ccggggctca gccaaaatgc ttcatcggcc caggaaccac
      421 ctttgtacac acgagcacgg tcgctcacca gggtgctgtg accataatcg tagtatgcca
      481 gagatgcggt atcaccatct tcatagttct tcacgtcacc ctttttgtag ttccggcggt
      541 taaccacttc ttcgtcggtt tgcggacgat agcgcacacg acccatgctg tcaagttcgg
      601 cgatgttgcc atcctcatcc agttctttgg tcatgtactt gttaccacgg aaggggttga
      661 ggtcttccat atcataatag gtaagcggac gatagacgtc gaacacccat tcgttcacgt
      721 taccggccat attgtacaga ccgtaatcgt tcggctgata ggagtagata ttagcagtaa
      781 cgatggcgtt gtcgttcagc ttaccggcca gaccggcata gtcaccgcga ccacgcttgt
      841 agttggccag catcttaccc tgcgaccagc gttctttgga agagggctcc cgcatggtat
      901 tgccatccca gggatagaga cgacggtcgg tgagcatttc attaccttct acctgattac
      961 cgatcagacc gagggaagcg tattcccatt cagcttctgt gggaaggcga tagtcaggca
     1021 gcaggatacc gtcttccatt ttgatggtac ggcccagctt ggaatcgccg gcttcggggc
     1081 tatagtcttt cacccctttc ttcacaccgg tggtataaac accggcataa taagattggg
     1141 tagtgaagtt gtcttcgttg taagagttga tgttgatgtc catatagcct ttgtcggcca
     1201 tgatggcttc gttcacgcgg tcacttctcc agcggcagaa ctcgttggcc tgcagccagg
     1261 agatacccac cacaggatac tcatcatagg aaggatagcg gaagtagtat tccacataag
     1321 gctcgttgta agcaagttct tcccgccata ccagtgtatc gggtaaggcg ttgtcgatga
     1381 tctcggggaa gtcgataccg aaggtgcgtg tggtccagta gaggtactcg cggtagtgga
     1441 ggttggacac ctcggtctca tccatataga aggaagagac ggtcaccctg cggggcacgt
     1501 tgtggaactc ataggtaaca tcctgttcgg tcatacccat ggtaaatgtt ccaccctgga
     1561 taagcaccag gttcggacct gtggcctgac cgcggtagtc aatgttttcg aaacctcccc
     1621 acttggtatc gttgtacttg aagccggtta ccgccgaccg gtcagcgcag gaggtaaaca
     1681 cagtacccgc caggaaaaca cccagtaccg ggacaatgat tcttttcata ccgtgcataa
     1741 tttggcaata aacgcaaatc taactataaa attaccgaaa ttaaaaaacc gaaggacggg
     1801 gcaaaacact gtagtatttg cctttggaat gctttccggc ctgatctaac cggaatatca
     1861 ggcccacttc atgtgacccg cccgtttcgc cggaaagtcc gcccaccgtg acatcatagc
     1921 tatacgtgaa caggatccag tcctttttca agccggcaag gatactaacg tcgctcatgc
     1981 cggagaaatt gtttttcaga aagaagcctc ccatgacaaa gcttttagca aaataggacc
     2041 ccagctgcaa ctgactgaat ccgccctgtg agatcagctg aactgtaggt tcagccataa
     2101 aaggatcccg tttccggtgt ttctgcttca ggaaaagccc accctgccag ctgatggcca
     2161 tgggcaggcg gtcttcttca taatcataga aggtattggg aggctggttc acatgcagaa
     2221 aagaaatgcc ggtgtaggcc ttttctgtga caaaagcagc
//
LOCUS       DAOMAX010000375         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11348_length_2253_cov_3.966788, whole genome shotgun sequence.
ACCESSION   DAOMAX010000375 DAOMAX010000000
VERSION     DAOMAX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11348_length_2253_cov_3.966788"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK742_12985"
     CDS             complement(<1..324)
                     /locus_tag="PK742_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQU77552.1"
                     /translation="MALDSKIPAGPLADKWTNYKNKVKLVNPANKRKLEIIVVGTGLA
                     GASAAATLAELGYKVKSFCFQDSPRRAHSIAAQGGINAAKNYKNDGDSVYRLFYDTVK
                     GGDFRS"
     gene            complement(333..1019)
                     /locus_tag="PK742_12990"
     CDS             complement(333..1019)
                     /locus_tag="PK742_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HQU77553.1"
                     /translation="MNGMNWFGKFLTSSIGRKFVMGLTGIALVLFLIVHCGINALIFV
                     NDGGVLFNEAAHFMGTNIIIRTMEIGLFVGILLHIVQAYMLAVANSKARPGKYQVNSA
                     NANSRWYSRSMTLLGTLILLFLIIHLKHFWVVSRFTGLEETSYGAGNLYIEMQEVFAI
                     GWVVIVYLIGVISLAWHLLHGFQSAFQTFGWNTLKYRGLIKCAGKVYAILIPLLFALM
                     PVAMYFEWIK"
     gene            1224..>2253
                     /locus_tag="PK742_12995"
     CDS             1224..>2253
                     /locus_tag="PK742_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77554.1"
                     /translation="MRLITRLLFIASFCMTAVQAQTVKVFLNTQVPEEGKFQGFQTVD
                     IFEECPPTSDTCMSWLAGLRPEVVRFPSGGQAKFTHLTTGPGYGFDLEEIDAYFYDLF
                     NCDEAYCGAGSNYEAAVNDWTQKCLAQDSLPGGGRYIDDFILMIQTLEAQLGYTIDVL
                     FCLNIVTADAAENKEALDLLIANGIHVTGAEIGNETYSESNGFESFESYLAYVQNPSG
                     AVGDQDYIGMIRQYFPDLKVGVCAAPPGTVDVEQGPGITYATPSEGKRIKYDDWNTAL
                     GSGWDSTRTISTPMPHTVPLYDAAVVHFYYKSSFWQDCAVQFVNDLDAYGGTCCPYSF
                     DAPDPRLTA"
BASE COUNT          542 a          559 c          615 g          537 t
ORIGIN      
        1 ggaacggaag tcgccgcctt tcacggtatc gtagaagaga cgatagacag aatctccgtc
       61 gttcttatag ttcttggctg cattgatgcc tccctgggca gcgatggagt gtgccctgcg
      121 gggagaatcc tgaaagcaga aggatttgac cttgtagccc agttccgcca gcgtagcagc
      181 agcggaggct ccggccagtc ctgtgcctac cacgatgatc tcaagcttgc gcttgttggc
      241 cgggttgacc agtttgacct tgttcttgta gttggtccac ttatcggcca aaggccctgc
      301 aggtatcttg gagtcgagtg ccatgtccgt aattatttga tccattcaaa atacatcgcc
      361 accggcatga gggcgaagag cagcggaata aggatggcgt acacctttcc ggcgcatttg
      421 atcagtccgc ggtatttcag cgtattccat ccgaaggtct ggaaggccga ctggaaaccg
      481 tgcagcagat gccatgccag gctgatgacg cctatcagat agacgatgac cacccagccg
      541 atggcaaaca cttcctgcat ttcgatgtag aggttgccgg caccatagct tgtttcttcc
      601 aggcctgtaa agcggctcac cacccagaaa tgcttcagat ggatgatgag aaagagcagg
      661 atgagcgtac ccagcagggt catgcttcgg ctataccagc ggctgttggc gttggcgctg
      721 ttcacctggt acttgccggg gcgggccttg ctgttggcta cggccagcat gtatgcctgc
      781 acaatgtgga gcagaatgcc cacgaacagg ccgatctcca tggtgcggat gatgatgttg
      841 gtgcccatga agtgggctgc ctcgttgaag agcacaccac cgtcattgac gaagatgagt
      901 gcgttgatgc cacagtggac gatgagaaac aggacgaggg caatgcccgt cagtcccatc
      961 acgaattttc tgcctatgct cgaggtcaga aacttgccga accagttcat tccgttcatt
     1021 ctttaacagt ggcaaatgta cataccagag ggcggaaatc ggaagatatt cagccttgat
     1081 atgcgatgac ttattaacaa aaaggtgcgg atgaggttcg caatgccaaa tgcaaacctt
     1141 tctgttgctg cccggccatg aaccgtcaaa cgggttatat ttgaacggac ctttttgcac
     1201 cgaagattcc aattaccctg ctcatgagac tgatcacacg gttacttttt attgcttcct
     1261 tctgtatgac cgctgtccag gcacagacgg tcaaggtttt tctgaatacc caggttccgg
     1321 aagagggaaa atttcagggc tttcagaccg tagatatctt tgaggaatgt ccgcccacct
     1381 cagatacctg tatgagctgg ctggccggac tcaggcctga agtggtgcgc tttccaagcg
     1441 gcggacaggc gaaattcacc cacctgacca ccggaccggg ttatggtttc gatctggaag
     1501 aaattgacgc ctatttctat gatctgttca actgcgatga ggcatactgt ggtgcaggat
     1561 ccaattacga agcagccgtt aatgactgga cacaaaaatg tctggcacag gactctctgc
     1621 ccggtggcgg gcgatacatt gatgatttca tcctgatgat acaaacgctg gaagcgcagc
     1681 tcggctacac cattgatgta ttgttctgtc tgaatatcgt gactgcagat gctgccgaaa
     1741 acaaggaagc gctggacctg ctgatcgcaa acggtatcca cgtgaccggt gctgaaatag
     1801 gaaatgaaac ctatagtgag tcaaatggct tcgaaagttt tgaaagttat ctggcatatg
     1861 tacagaatcc ctccggtgct gttggcgacc aggattacat tggcatgatc aggcagtatt
     1921 ttccggatct gaaagtgggt gtatgtgccg cacctcccgg cactgtagat gtggaacaag
     1981 gccccggcat tacctatgcc acaccatctg aaggcaaaag gatcaaatac gacgactgga
     2041 acaccgcttt gggaagcgga tgggacagta ccaggaccat cagcaccccg atgccacata
     2101 ctgttccgtt gtatgatgca gcagtggtgc acttttatta caaaagcagt ttctggcagg
     2161 attgcgcagt gcagttcgtg aacgacctgg atgcctacgg aggaacctgt tgtccgtatt
     2221 cctttgatgc gccggaccct cggctgacgg cag
//
LOCUS       DAOMAX010000376         2241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11456_length_2241_cov_3.886093, whole genome shotgun sequence.
ACCESSION   DAOMAX010000376 DAOMAX010000000
VERSION     DAOMAX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11456_length_2241_cov_3.886093"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1311)
                     /locus_tag="PK742_13000"
     CDS             complement(193..1311)
                     /locus_tag="PK742_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77555.1"
                     /translation="MRKLIFLLIKEFQQIFRNPAIIRIIFVMPAVQLLVLPLAADYEV
                     KNVYVTVVDHDHSDYSRQLIEHIRSSNYFLLTGYADSYQAALKDVEDDRSDIILEIPD
                     GFERDLVRNDEVTLNLSANAVNGAKGGIGAAYAASLIRQFNNEIRMDWAQLPRYSPIA
                     MIEVTGSNWFNPDMDYALFMVPGILVILLTMVGSFLTSINIVYEKETGTIEQINVSPI
                     RKYQFILGKLIPFWLIGFVVLTIGLAIARLAYGIIPEGSFGLIYLFAAVYLLAFLGIG
                     LLLSTYAETQQQAMFIAFFLMMVFILMGGLYTSIDSMPEWAQVIARLNPVSYFIEVMR
                     MVVMKGSTIADTGRHFAIIGGMAVFFNVWAIVNYRKRI"
     gene            complement(1308..>2241)
                     /locus_tag="PK742_13005"
     CDS             complement(1308..>2241)
                     /locus_tag="PK742_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77556.1"
                     /translation="ATRQLTERLDASRYFNLALVTDNMAEAEQAFRRDKIKLLVVFEP
                     DFAHKLEHEHVAGLQLLADATDPNTATTLRNYAGAIIQDYQQEVWGEQVLPNRMEVET
                     HMLYNPQLIGAYNFVPGVMGMIMLLISAMMTSIAIVREKELNTMEVILASPLKPFLLI
                     TSKAVPYVVLSLVNVSTILLISTTLLDLPIRGSLLLIYAESFLFISTAISLGLMISNI
                     TDKQQTALFISLMGLMLPTLILSGFMFPIENMPKPMQVLSNIVPTKWFFIIIKNLMIK
                     GLGWESVWKQSLILLGMTFFFLAVSVRTFKRRLA"
BASE COUNT          569 a          574 c          585 g          513 t
ORIGIN      
        1 atacagccct gaccaaatgg tcggggcttt ttttttggca aaagggtaaa accccgatag
       61 tgatcgggat aatgggtggc tgccctccct gtgaccaggc agactaaaaa ctaataacca
      121 agaaccaaaa actaataacc caaaaccaaa aactaataac ccaatcccaa agaccaggaa
      181 ccagcaacca attcagatcc tcttcctgta attcacgatc gcccagacat tgaagaacac
      241 cgccatgcca ccgatgatgg caaaatgacg gccggtatct gctatggtgc tgcccttcat
      301 gactaccatc cgcatcacct cgataaagta tgacaccgga ttgagccggg caattacctg
      361 agcccattcg ggcatgctgt caatgctggt gtacaagccc cccatcagta tgaagaccat
      421 catcaggaaa aaggcgatga acatggcctg ctgctgggtc tctgcgtagg tgctaagcaa
      481 caaaccgatg cccaggaatg ccaggagata gaccgccgca aacaggtaga tcaatccgaa
      541 actgccttcc ggtatgatgc catacgccag ccgggcaatg gccagcccga tggtgagtac
      601 cacaaaaccg atcagccaga acggaatgag tttacccagt atgaactggt acttcctgat
      661 gggcgatacg ttgatctgct cgatggtacc ggtctctttt tcgtacacga tgttgatgga
      721 ggtcaggaaa ctacccacca tggtgagcag tatcacgaga atacctggca ccatgaacaa
      781 ggcatagtcc atatcgggat tgaaccagtt gctgccggtc acctcgatca tggcgatggg
      841 actgtacctt ggtagctgtg cccagtccat acggatctca ttgttgaact gcctgatcag
      901 actggctgca tacgccgcac cgataccacc tttggcaccg ttcacggcat tcgccgacag
      961 gttcagggtc acctcatcgt tccgcaccag atctcgttca aaaccatccg gaatttccag
     1021 gatgatatcg gaacggtcgt cttccacatc tttcaatgct gcctgatagg aatctgcata
     1081 tccggtcagc aaaaagtaat tggacgagcg tatatgttca atgagttgcc gtgaataatc
     1141 agagtgatcg tgatccacca cggtcacata gacattcttt acttcataat cggcagcgag
     1201 gggtaacacc agcagctgca ccgccggcat cacgaagatg atccggatga tagccgggtt
     1261 ccggaagatc tgctggaact ctttgatgag cagaaagatc agttttctca tgccagtctg
     1321 cgtttaaagg tgcggaccga tacggccagg aaaaagaagg tcatgcccag taagatgagc
     1381 gactgtttcc agacgctttc ccatcccagc cctttgatca tgaggttctt gatgatgatg
     1441 aagaaccact tggtgggcac tatgttgctc agcacctgca tgggtttggg catgttctca
     1501 atggggaaca tgaaaccact gagaataagt gtcggcagca tgagtcccat gaggctgatg
     1561 aacagtgccg tttgctgttt atcggtgatg ttgctgatca tcagtccgag gctgatggct
     1621 gtgctgatga acaggaaact ctctgcatag atgagcagca ggcttccgcg gatgggcagg
     1681 tccagcaggg tggtggatat aagaaggatg gtgctcacat tgaccagcga cagtaccaca
     1741 tacggaacgg ctttgctggt gatgagcaga aatggtttga gcggggaagc caggatcact
     1801 tccatcgtat tgagttcttt ctctctgacg atggcgatgc tggtcatcat ggcggagatg
     1861 agcagcatga tcatacccat gacacccggc acgaaattgt aagcacctat cagctgtgga
     1921 ttgtacagca tgtgggtttc cacttccatg cgattgggca gcacctgttc gccccacact
     1981 tcctgctgat aatcctggat gatggccccg gcgtagttgc gcaaagtggt ggcggtattg
     2041 gggtcagtgg catcggccag caactgcagt cctgccacat gttcgtgttc cagcttatgt
     2101 gcgaaatccg gttcaaagac caccagcagc ttgatcttgt cgcggcggaa ggcctgttct
     2161 gcttcggcca tattgtcggt caccagtgcc aggttgaagt agcgactggc atccagtctc
     2221 tccgtcagct gacgggtggc t
//
LOCUS       DAOMAX010000377         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11471_length_2240_cov_2.972540, whole genome shotgun sequence.
ACCESSION   DAOMAX010000377 DAOMAX010000000
VERSION     DAOMAX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11471_length_2240_cov_2.972540"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PK742_13010"
     CDS             complement(<1..1243)
                     /locus_tag="PK742_13010"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQU77557.1"
                     /translation="MSAYSPRSVWAFIVRTRLRRSISVGGLLLILSLWSLKNIGFSYH
                     FDDFFPKDDEDLEFYYQYRDQLAADDNFLLIGLSTPSALDPEFLHALDTASTLLAKSG
                     QTQRVASLTTYRYLWGNSMGMFPVPLVHPDDPERRMDDSVRIVSDPLVCGKLVSADMQ
                     TVVIATTTPDSMDLADNQALLADVEEAMTVAGFDDYHLLGKANFESELIRFQQEEFIR
                     FSILSVLCVALISFLLFRDLRIVGLSMLTVTVTLVFFLGLLGLTHRTLNVMSSLYPII
                     IIIVGISDMVHFVTRYQTEREAGFHPRRALGRMLQDVGFATFLTSVTTAIGFLTLLSS
                     PIPPIREFGILAAAGVLLAYLVTLLFAAPMMMLLPLPQKAEQGRSYFIARITNRIYRS
                     GKKRPVLTAGVSLLVVLLGILG"
     gene            1371..1745
                     /locus_tag="PK742_13015"
     CDS             1371..1745
                     /locus_tag="PK742_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77558.1"
                     /translation="MKKYVLSFLVVSLLTAGLAFAQEALPVASFNDHLELVLPADAPV
                     ASAYTADISDMGFKNKMAAEKFFRSVTDNLVYTELNYEESVVTIHLRLEYAREGWVAA
                     DWNNYFVQASERYRRSYNYFNQ"
     gene            1758..>2240
                     /locus_tag="PK742_13020"
     CDS             1758..>2240
                     /locus_tag="PK742_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77559.1"
                     /translation="MKQGRLYHWILTGILSGICILPAKAQIVGSDVFLQGLFVEVGIN
                     QCAAYGSEGGAPAGYHPTESGLGFVADWESDGWDTGTPDYCVDYFVPGSPVEGWQVQV
                     GTDVWTNTDQFCSPDEIPGDITSYEFADGVYTAVWEGDIASENLAITQTTRLPEDKLY
                     F"
BASE COUNT          618 a          533 c          553 g          536 t
ORIGIN      
        1 caccaagaat acccagcaaa acaaccagaa gggaaacccc cgctgtcaga acaggtcgct
       61 tttttccgga acggtagatc ctgttggtta tccgtgcaat gaaatatgac cgaccctgct
      121 ctgccttctg tggtaatgga agcagcatca tcataggagc tgcaaacaac agcgttacca
      181 gatatgccag caagactcct gcagcggcca gtattccaaa ttccctgatt ggaggtatgg
      241 gagaactcag caaggtcaga aaacctatgg ctgtggtcac tgacgtaaga aaggtagcaa
      301 aacctacatc ctgaagcatt cggcctaagg cccgtctggg gtggaagcct gcttcccgtt
      361 cggtctggta ccgtgttaca aaatgtacca tatcactgat gcccacaata atgatgatta
      421 ttggatacag cgaactcatc acattgagcg ttcggtgggt tagtccaagc agacccagga
      481 agaaaaccag cgtcactgta acggtaagca tggataagcc aacaatacgc agatcacgga
      541 acaacaggaa ggagatcagg gccacacaca gcactgacag gatggaaaac cggatgaact
      601 cttcctgttg aaatcgtatc agttccgatt cgaaattggc ctttcccagc aaatggtagt
      661 catcaaatcc ggctacggtc atagcctctt ccacatctgc cagcaatgcc tgattatccg
      721 caagatccat agaatccggg gtggtcgtgg ctatgaccac cgtctgcata tctgcagata
      781 ccagtttgcc gcaaaccagc gggtcactga ctatccggac actgtcatcc attctgcgtt
      841 cagggtcatc aggatgcacc agcggaaccg gaaacatgcc catggagttg ccccataaat
      901 accggtaggt ggtcaatgag gctacccgtt gggtttgacc actcttggcc agcaaggtgc
      961 ttgctgtgtc aagtgcatgt aaaaattccg gatccagggc agagggtgtg gataacccga
     1021 tcaacaggaa attatcgtct gcagcaagct gatctcggta ctgatagtag aactccaggt
     1081 cttcatcatc tttaggaaaa aaatcatcga aatgatagga aaagcctatg tttttaagag
     1141 accacagaga caggataagc aacaatcccc caacggaaat gctcctccga agccttgttc
     1201 taactatgaa ggcccagaca gaacgagggg agtatgcaga catatcagat caggttggag
     1261 gtttttctgt acagatgacc ccggtagaac gtttgaaacg gcttttggtt atacgatatg
     1321 ataatcttaa ttttagcgac tgaataagaa ccatttaaga accttatctt atgaaaaagt
     1381 acgtactcag ctttttggtt gtcagcctcc tcacagcagg cctggctttt gcacaggaag
     1441 cattgcctgt tgcctctttc aacgaccacc tggaactggt cctgcctgcc gatgctccgg
     1501 ttgcatctgc ctacacggca gacattagtg atatggggtt caagaacaag atggcagcag
     1561 aaaaattctt ccggagtgtt acagacaatc tggtgtacac tgaactgaat tatgaagagt
     1621 ctgtagtgac cattcacctc cgcctggaat atgccaggga aggatgggtt gcagctgact
     1681 ggaataacta ttttgtccag gccagcgaac gttacagaag gtcttacaac tatttcaacc
     1741 agtagtaccc gcatcctatg aaacaaggga gactttacca ctggatactt acgggtatcc
     1801 tttcaggcat ttgcatactt cctgcaaagg cacagatcgt aggatctgat gtgttccttc
     1861 agggtttgtt cgtagaggtt ggtatcaatc agtgtgctgc ttacggctct gaaggaggtg
     1921 ctcctgccgg ataccatccc actgaatcag gactcggatt tgtggctgac tgggaaagcg
     1981 acggatggga taccggtacg ccggattatt gcgtcgatta tttcgtaccc ggttctccgg
     2041 tggaaggctg gcaggtgcag gtaggtacag atgtatggac caatacagac cagttttgtt
     2101 ctcccgatga aattcctgga gatataacca gctacgaatt tgcagacggc gtttataccg
     2161 ctgtctggga aggtgatata gcttctgaaa acctggctat cacacagacc accaggctgc
     2221 cggaagataa actgtacttc
//
LOCUS       DAOMAX010000378         2236 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11502_length_2236_cov_4.454379, whole genome shotgun sequence.
ACCESSION   DAOMAX010000378 DAOMAX010000000
VERSION     DAOMAX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11502_length_2236_cov_4.454379"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2236)
                     /locus_tag="PK742_13025"
     CDS             complement(<1..>2236)
                     /locus_tag="PK742_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77560.1"
                     /translation="GAELANVLTTVYDRFGDVKWQAEYNSSYDDNDYGTAIAIDDDLN
                     VYVAAASYTSATNYYDYLVIKYDSTGSELWTATYNGPGSLYDIPSDILIDGSGNVYVT
                     GGSVGSGTGWDYATVKYNSSGTQQWVSRYNYNDHDDAASGLATNSSGQILVTGASAQA
                     DDNWDFASVKYNQSTGAQITAQRNTASGLGIDRVNSIAIDADDNVYLTGRAAITSEGY
                     NIRTIKLDSLLATIWNKQYNLADLDDEGMSVWVDGEGNVYVTGTATADNDKTRGVTIK
                     YNSAGTLQWTVLETPNNGEALNSSNRAMSISEDGQIMVAGTAENGADTDIMTYVYDED
                     GNRLWAERWDGGDDDQASFIHADGDNEIFTGGISQDGGSSTYTLIKYRRAAYITPPDD
                     DPDKPAYAGWWENRGQIIDTDDNIRTDIKYYADRHYPALYATDDSLFMVFSRIDTSTT
                     TDDTLARIDMGFVGSSSTKEIMKTTSQGGEYRNFFLAHTGADGVTNVQKTDRLYTKDL
                     YPKVDLMHYFDQAGVKFYLIIQPGYSQQNDPIEMVFNGADITFNADTTLTLETEIGGI
                     TLRRPEAYQIETDGSRTDLTWHPEYTDEGSGVVGLSLGSYTTTKVLVLEFGVEKELLS
                     LASCEDNVIWGTYWGGDQNESIEDIKFDYIPAAMTPGTSTITIAGFTRSANFPEEIFM
                     DTELSGDVGDIFVQKFNSTYVPQWGVVIGGSSVEGNIFPFAKIDTWNSPDGRGYTPLV
                     YASFS"
BASE COUNT          458 a          603 c          546 g          629 t
ORIGIN      
        1 gagaaagagg catatacaag aggcgtatat ccccttccat caggtgagtt ccaggtatca
       61 attttcgcaa atgggaaaat atttccttca accgagctgc caccaatcac aacaccccat
      121 tgtggcacat aagtggaatt gaatttctgt acaaatatgt ctcctacatc acctgataat
      181 tcagtatcca taaaaatctc ttcgggaaaa tttgcactcc tggtaaaccc tgcaatagta
      241 atggtagaag tcccaggagt catagctgca ggtatgtaat caaatttaat atcttcaatt
      301 gactcattct gatcaccgcc ccaataggtg ccccagatca cattatcttc acagctagcc
      361 agggacaaga gctccttctc caccccaaat tccagcacca acaccttagt agtagtataa
      421 ctgcccaggc tcaaccccac taccccgctt ccttcatcgg tgtattccgg gtgccaggtc
      481 aggtcggtgc ggctgccgtc tgtctctatc tggtaggcct ccggtcttct gagggtgatg
      541 ccgcctatct cggtttccag ggtcagggtg gtgtcggcat tgaacgtgat gtctgcaccg
      601 ttgaacacca tttctatagg gtcgttctgc tggctgtagc cgggctggat gatgaggtag
      661 aatttcactc ctgcctggtc aaaatagtgc atcaggtcca ccttggggta gaggtctttg
      721 gtgtataacc tgtcggtttt ctgtacgttg gttaccccat ctgctccggt gtgtgccagg
      781 aagaaattcc tgtactcgcc gccctggctg gtggtcttca tgatctcttt ggtgctgctg
      841 ctgcccacaa agcccatgtc tatcctggcc agcgtatcat ctgtggtggt actggtgtct
      901 atacggctga agaccatgaa aagactatca tctgtggcat acaaggccgg gtagtgcctg
      961 tcagcgtagt atttgatatc tgttctgata ttgtcgtccg tgtcaatgat ctgtcccctg
     1021 ttctcccacc agccggcata ggcaggttta tccggatcat cgtctggtgg cgtgatatag
     1081 gctgcccttc tgtatttgat cagggtatag gtggagctgc caccatcctg gctgataccg
     1141 cctgtaaaga tctcattgtc gccatcagca tggatgaaag aagcctggtc gtcatcaccg
     1201 ccatcccagc gctctgccca caaccggttg ccatcctcat catagacata ggtcatgata
     1261 tccgtatcgg cgccgttctc tgctgttccc gctaccatga tctgtccgtc ttcactaatg
     1321 ctcatggccc tgttgctgct gttgagggct tccccgttat tgggcgtttc caggactgtc
     1381 cactgcaggg tgcccgcact gttgtacttg atggtgactc ctcttgtttt atcattgtct
     1441 gccgtagccg tgcccgttac atagacattg ccttcgccat ctacccagac ggacatacct
     1501 tcatcatcga gatcggccag gttgtactgc ttgttccaga tggtggccag caggctgtct
     1561 aatttaatgg tgcggatgtt gtagccctct gacgtgatgg ctgccctacc ggtcagatat
     1621 acattgtcat ctgcatctat ggctatgctg ttcacccggt caatgcccag accggaggcg
     1681 gtattccttt gtgctgttat ctgggctccg gtactttggt tgtatttcac actggcaaag
     1741 tcccagttat catctgcctg ggcactggca cctgttacca ggatctgtcc cgagctattc
     1801 gtggccagac ccgaggcggc atcatcatgg tcattgtagt tataccggct cacccattgc
     1861 tgggtacctg agctgttgta tttgacagtg gcataatccc agccggtacc tgagcccaca
     1921 ctgcctcctg ttacatacac gtttccggag ccatcgatca gtatatctga tggtatgtca
     1981 tacagactgc ccggtccgtt gtaggtggcc gtccacagtt cagatccggt gctgtcatac
     2041 ttgatgacca ggtagtcata gtagttcgtg gctgaggtgt agctggcagc agccacatag
     2101 acattcaggt catcgtctat ggcaatggcc gtaccgtaat cgttgtcatc atagctgctg
     2161 ttgtactctg cctgccattt gacatcgcca aaccggtcat agaccgtggt gagcacattg
     2221 gccagttctg cgccgg
//
LOCUS       DAOMAX010000379         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11514_length_2235_cov_3.550917, whole genome shotgun sequence.
ACCESSION   DAOMAX010000379 DAOMAX010000000
VERSION     DAOMAX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11514_length_2235_cov_3.550917"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..2132
                     /gene="glgB"
                     /locus_tag="PK742_13030"
     CDS             228..2132
                     /gene="glgB"
                     /locus_tag="PK742_13030"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQU77561.1"
                     /translation="MSEYVIHGPSLFTEYDIHLFKEGKHFRLWEKMGAHLIEHEGEEG
                     VLFSVWAPNAEKVCVEGEFNYWNKDNYPLQARWDESGIWEGFVPGLKPGTLYKYAIHQ
                     KDGKETLEKCDPYSRMNETPPKTASVVVDSRYDWTDGRWLEAREEQGYERPMSIYELH
                     LGSWRRDEDGNSYSYRRMAEELVPYLKETGFTHVEFMPVMDHPFFGSWGYQVIGYFAP
                     SSMYGEPDDLKYLIDKLHEAGIGVIMDWVPSHFPGDAHAIKEFDGTHLYEHEDLRRGF
                     HPDWNSYIFNYGRYEVRSFLISSALFWLLEYHADGLRVDAVASMLYLDYSRKEGEWLP
                     NDFGGNENLEAIELLKMMNDAVHEDAPGCVTIAEESTAWPKVSGPTSDGGLGFDYKWM
                     MGWMHDSLRYFGREPIYRKFHQNELTFSIWYFFNEHFQLAFSHDEVVYGKGSLWNKMS
                     GDEWHKFANYRLLLSYMYTHPGNKLIFMGSEFAQREEWNHDAQLSWTSLDDERHRKLK
                     DMVARLNTLYAEQLPLHECDFEEAGFKWTQLDRQDDCILCYERIAADEEDHLLVVMNA
                     DQQTHFDYPVNVREGVSYQELFNSDDEAWGGSGLVNEGVLEPVKNESEQDEIRLLVPP
                     LSLIILQPKK"
BASE COUNT          564 a          512 c          647 g          512 t
ORIGIN      
        1 cagaaaggct gcgatctcca ggctgtccgt ttgaggtatg gggatgctca ccaaggtgtc
       61 ttccactaca ggaataacag gcgggattac cggtgcggga tcagcatggg tacaggagct
      121 aactatcgtc agcaggaggc cgatggcaat gggaaaggaa agcaggtgtt tcatggttca
      181 ggtagcatga aaattacaag ctttatgcca aacagtatct ttgccgtatg tcagaatatg
      241 tcatccacgg tccgtcgctg ttcacggaat atgatatcca tcttttcaag gaaggcaagc
      301 atttccgcct ctgggaaaaa atgggcgcac atttgatcga acacgaaggg gaagaaggtg
      361 tgctcttttc tgtctgggcg ccgaatgcgg agaaggtttg tgtggaaggg gagttcaact
      421 actggaacaa ggacaactat cccctgcagg ccaggtggga tgagtctggt atttgggaag
      481 gatttgtgcc gggactgaaa ccgggaacac tgtataaata tgccattcat cagaaggacg
      541 ggaaggagac gctggagaaa tgcgatccgt attccaggat gaatgagaca ccaccgaaga
      601 ccgcttctgt ggtggtggat agccgttatg actggaccga cggccgatgg ctggaagcac
      661 gggaagagca gggctacgaa cgcccgatgt cgatctatga actgcatctg ggcagctggc
      721 ggagggatga ggacggcaac tcctacagct atcgccgcat ggctgaagag ctggtgcctt
      781 atctgaaaga gacaggtttc acgcatgtgg aattcatgcc cgtcatggac catccatttt
      841 tcggttcctg ggggtatcag gtgatcgggt attttgctcc gtcatccatg tatggtgaac
      901 cggacgatct gaaatacctc atcgacaaac tgcatgaggc aggcatcggg gtcatcatgg
      961 actgggtgcc ctcgcatttt cccggcgatg ctcatgccat caaagagttc gatggaacgc
     1021 acctgtatga acacgaagat ctgcgtcgcg gatttcatcc tgactggaac agttacatct
     1081 ttaactatgg ccgctatgag gtgaggagtt ttctcatcag ctctgcactg ttctggttgc
     1141 tggaatacca tgccgacggt ttgcgggtgg atgcggtggc cagtatgctc tacctcgatt
     1201 attccaggaa ggaaggagag tggttgccga atgactttgg tgggaatgag aacctcgaag
     1261 ccattgagct gctgaagatg atgaacgatg cggtacatga agatgcaccg ggttgtgtga
     1321 ccatagccga agaaagcacg gcctggccga aggtgagcgg accaacctcc gatggcggat
     1381 taggttttga ctataaatgg atgatgggct ggatgcacga cagcctgcgc tacttcggtc
     1441 gcgagcccat ctacaggaaa ttccaccaga acgaactgac cttcagcatc tggtatttct
     1501 tcaatgaaca ttttcagctg gccttttcgc atgacgaggt ggtttatggc aaaggctcat
     1561 tgtggaacaa aatgagcggc gatgagtggc ataagtttgc caactatcgt ctgttgcttt
     1621 cctacatgta cacccatccc ggcaacaagc tcatcttcat gggcagtgaa tttgcacagc
     1681 gggaagaatg gaaccacgac gcacaattga gctggacatc tctggatgat gaacgacaca
     1741 ggaaactgaa agacatggtg gccaggttga ataccctgta tgcggaacaa ctgccactgc
     1801 atgagtgcga ctttgaagaa gcgggattca aatggaccca gctggaccgc caggatgatt
     1861 gcatactttg ttatgaacgc attgctgcag atgaagagga tcacctgctg gtggtcatga
     1921 acgcagatca gcagacgcac tttgattatc ccgtcaacgt gcgggaaggg gtcagctacc
     1981 aggagctctt caattcagac gatgaggcct ggggcggatc gggactggtc aatgaaggtg
     2041 tgctggagcc agtaaaaaat gaaagtgaac aggacgaaat aagactgctg gtgccgccgc
     2101 tcagcctgat catcctgcaa ccgaagaaat agaggataca cctctcgcta actgtttctc
     2161 cggcccccga gccggagtcc ccctcttcct ttgaaactgt ttctccgggc ttgacccgga
     2221 gtctccctct ttatc
//
LOCUS       DAOMAX010000380         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11670_length_2216_cov_4.256826, whole genome shotgun sequence.
ACCESSION   DAOMAX010000380 DAOMAX010000000
VERSION     DAOMAX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11670_length_2216_cov_4.256826"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PK742_13035"
     CDS             <1..478
                     /locus_tag="PK742_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77562.1"
                     /translation="SWMPILFRIDEEGPVWDSVHFLPPQPEHFCFVHLNQKQNSRDAI
                     AAFRSGKNKFEKESHIISEISEALLFCDDFADFLTLLDEHEEVMQFVLQEERVQTARF
                     QKFNGVVKSLGAWGGDFVLAGSDMPPAEMHQWFKKQGYETVLPYTDMVLMEKPEGK"
     gene            complement(552..1874)
                     /locus_tag="PK742_13040"
     CDS             complement(552..1874)
                     /locus_tag="PK742_13040"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HQU77563.1"
                     /translation="MSTIKGFSKLSRTDKIAWLAKHFLTSNPLDIAREFASFMHTNSE
                     TQRLFDGISENTLTNFYMPYGVAPNFVINGRTYVVPMVIEESSVVAAAASGAKFWSER
                     GGFHAEVVSTTKVGHVHFQWRGDSDKLFTFFSDIKDILILEVSHLTQNMEARGGGLKN
                     IELIDCTHLEPEYYQLKATFETCDAMGANFINSVLEEFARTLEEEAKVYPEFSGAEKK
                     IEVVMSILSNYTPESVVKAWVECPVEELGSFPEADLDAQAFAKKFRTAVNIANVDVSR
                     ATTHNKGIFNGVDAVVLATGNDFRAIEACGHAYAARDGQYRSLSKVSIIDGIFRFEIE
                     LPLAVGTVGGLTRLHPLSKRSLEMLGNPGAKELMKIMASVGLAQNFAAVKALVTSGIQ
                     KGHMKMHLTNILRHFNATDLEIENALQYFADKTVSFSGVREYLVKVRG"
     gene            complement(1903..>2216)
                     /locus_tag="PK742_13045"
     CDS             complement(1903..>2216)
                     /locus_tag="PK742_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77564.1"
                     /translation="KTDQELFEPLAFTGHTAEEMLWFVNDILDDEHARIACKHVIMSG
                     GIRHFLDGYAGIEQLRCPAVYAQGSAFLKHAMGDYDALRAYMEGQSRGLLLAKAYLKV
                     R"
BASE COUNT          519 a          601 c          553 g          543 t
ORIGIN      
        1 gagttggatg cccatcctct ttcgcattga tgaagaaggt cccgtgtggg acagtgttca
       61 cttccttcct ccgcaacccg aacatttctg tttcgtccac ctcaatcaga aacagaacag
      121 ccgtgatgcg atcgctgcat tccgttccgg aaaaaataaa ttcgaaaagg aaagccacat
      181 catctctgag atcagtgaag cactgttgtt ctgcgatgat ttcgccgact tcctcaccct
      241 gctcgatgaa cacgaagaag tgatgcagtt cgtgttgcag gaagaacggg tgcaaacagc
      301 caggttccaa aagttcaacg gcgtggttaa atcactcggt gcctggggtg gcgatttcgt
      361 cctggccgga tcggacatgc cacccgctga gatgcaccaa tggttcaaaa agcaaggcta
      421 cgaaacggta ttgccttaca cagatatggt cctgatggaa aaaccggaag ggaaataaac
      481 cggaattcgg aataactgct aaccgcctac tgccaattgc caatcccgat agtgatcggg
      541 atgaactgat cctatcctct taccttcacc agatactccc ttacaccgct aaaactcacg
      601 gtcttgtcgg cgaagtactg cagagcattc tcgatctcca gatcggtagc gttgaagtga
      661 cgcaaaatat tggtcaggtg catcttcatg tggcctttct ggataccact ggtcaccagc
      721 gccttgacag ctgcgaagtt ctgtgccagt cccacactcg ccatgatctt catcagttcc
      781 ttggcgccgg ggttacccag catttccagt gaacgcttgc tgagcgggtg cagacgtgta
      841 agaccgccaa cggtacccac tgcaagcggc agttcaatct caaaacggaa gatgccgtca
      901 atgatactca ctttactcag acttcggtat tgtccgtcgc gggcagcata ggcatgaccg
      961 catgcttcaa tggcccggaa gtcgtttcct gttgccagca ccactgcgtc cacaccgtta
     1021 aagatgcctt tgttgtgcgt tgtggcacgt gatacatcta cattggcgat gttgaccgcc
     1081 gtgcggaatt ttttagcgaa cgcctgtgca tcgagatcgg cttcagggaa actgcccagc
     1141 tcttctaccg gacattccac ccaggccttc accaccgatt ccggggtgta gttgctcagg
     1201 atgctcatga ccacttcgat cttcttctcg gcaccactga attcaggata caccttcgcc
     1261 tcttcttcca gggtgcgggc aaattcttcc agcacactgt tgatgaagtt ggcacccatg
     1321 gcatcgcagg tctcaaaggt ggccttgagc tggtagtact ccggttccag atgggtgcag
     1381 tctatgagtt cgatgttctt cagtccgccg ccgcgagctt ccatgttctg ggtcaggtgg
     1441 ctgacctcga ggatcaggat gtctttgata tcagaaaaga atgtgaagag cttgtcgctg
     1501 tcgccccgcc actgaaagtg cacgtgtccc actttggtgg tgctcaccac ttccgcatgg
     1561 aaaccacctc tttcactcca gaatttcgca ccgctggcag cagctgccac cacagaactt
     1621 tcttcaatga ccatgggcac cacataggtg cgaccgttga tcacgaagtt gggggccacc
     1681 ccgtagggca tatagaaatt ggtcagggta ttttcagaga taccgtcaaa gagacgctgg
     1741 gtctctgagt tggtatgcat gaagctggcg aattcccggg caatatcgag cggattgctg
     1801 gtcaggaagt gcttggccag ccaggcgatc ttgtcggttc gcgacagttt ggaaaatccc
     1861 ttgatggttg acatgcttgt tatttcgtgt ctgaacgcaa agttaacgca cttttaagta
     1921 agccttcgcc agcaataaac cgcgagactg tccttccatg taggcacgca gggcatcata
     1981 atcgcccatg gcatgcttca gaaaggcaga accctgtgca tatactgctg gacaacgcag
     2041 ttgctctatc ccggcatagc catccagaaa atggcggatg cctccgctca tgatcacatg
     2101 cttacaagct atacgggcat gttcgtcgtc cagtatatcg ttcacaaacc acaacatctc
     2161 ctcggcggta tgtcccgtaa aagccagagg ttcaaacagc tcctggtcgg ttttgt
//
LOCUS       DAOMAX010000381         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11774_length_2205_cov_3.954419, whole genome shotgun sequence.
ACCESSION   DAOMAX010000381 DAOMAX010000000
VERSION     DAOMAX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11774_length_2205_cov_3.954419"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PK742_13050"
     CDS             complement(<1..821)
                     /locus_tag="PK742_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77565.1"
                     /translation="MKIAWSIVLMSLCCWSCNQGRTGTDSHSYPVSREILMDNWRNAE
                     KLLVVYPSDDTIVRHFMEQYLVQFGTGRRAAPFELISDIEVNDSLLARYPAMLIGHFS
                     ADSKCRELIDLPYFSWMNKGFSFMGKQFRSEDDILRLSFVPNARYPDRPLMLITGNSN
                     SKIVAQFSSRNQEFGNYLLWDSWGYQIFHNGQRIMLGMLNDRFERDDVKSWEFDFRGK
                     VIAHNEHFDFYDHNSGLSELQTDSIMAYTHRNITAFETVFGVTAGGPFAYHLLPST"
     gene            1226..1849
                     /locus_tag="PK742_13055"
     CDS             1226..1849
                     /locus_tag="PK742_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77566.1"
                     /translation="MDNKLAELAEKIYQESIEKSRLDAEEIIQNARHEADNILREAKN
                     HKDALLHHAEQEAAGWKARALAEIRMLTDNALTALREEITHMIRYRVIDEPIHQILDQ
                     KEHVAVLLTEALKHLHEGEDGQIQVQLPDSFDQEATAYLQEEVGRQLSKEPELGLTGG
                     HFRGFAIKMKGKGFTIDFTEESFRSLLSAYFNQEMNDLLGNEASRES"
     gene            1827..>2205
                     /locus_tag="PK742_13060"
     CDS             1827..>2205
                     /locus_tag="PK742_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77567.1"
                     /translation="MKPPVNHEYYGLITGLPDLTLQKPDLAGTGNIWAQYGHLLTSHD
                     QLLLQLAYLPVDHKLVLCSFFEQPVSETDQLLACYDSSAHQSYLQQNLKLFPYQDQML
                     FPDKESPRISLEASWRIAWTTFVR"
BASE COUNT          617 a          514 c          501 g          573 t
ORIGIN      
        1 gtgctgggta acaggtggta ggcaaaaggc cctccggcag taactccgaa aacagtttcg
       61 aacgctgtta tatttcggtg ggtgtatgcc atgatactgt ctgtttgcag ctcagacaat
      121 ccggaattgt ggtcatagaa gtcaaaatgc tcgttgtgtg caatcacctt cccccggaaa
      181 tcaaattccc agctttttac atcatcccgt tcaaaccggt cattgagcat ccccagcatg
      241 atgcgctgtc cgttatgaaa gatctggtac ccccaggaat cccacaacaa atagttgccg
      301 aattcctgat ttctgctgct gaattgtgcc acaattttac tgttggaatt tccggtgatc
      361 agcatcagcg gccggtcggg gtaacgggcg ttcggcacaa aagacaagcg aagaatatca
      421 tcttctgaac gaaactgctt gcccatgaaa ctgaagcctt tattcatcca gctgaagtat
      481 ggcaggtcta tcagttcccg gcatttcgaa tcggcagaaa aatgtccgat cagcatagca
      541 ggatatctgg ccagcaaact atcgttcact tcgatatcgc tgatcagttc aaatggcgct
      601 gcacgtctgc ctgtgccaaa ctgcaccaga tattgttcca tgaaatgcct gacgatagtg
      661 tcatctgaag ggtacaccac cagcaacttc tctgcattcc gccagttgtc cattaatatc
      721 tctctgctta cgggataact gtgagaatca gttcctgtgc gtccctgatt gcatgaccag
      781 caacacaatg acattagtac aatagaccag gcaattttca tcactcagaa agttgaagtt
      841 ttttctgcag gaaaaaaaat ctgttttcag ttggttgaaa acggaacctg ctattatctt
      901 agtaccaacc gaaaggtatc agatccatat ctgcgttatt tgtttggtga tctaacattg
      961 gtaattcaac cgagaaaacg tccggtgtgt aatggcgtgc cgtaagaagt tgctgcctgc
     1021 agtaatgtat gaaccggatt acaaagcacc ccggcgcctc aaatcctgtg ggatgaggtc
     1081 actccaaaca gagcgtgata tggatctgtt ttttttacgg atagctgacc aatttctgct
     1141 gatctgacta tgtattccgg aagcgaacca gacttcctga tcatttctct aaagacattg
     1201 gtttgcaacc attaagtcag acagtatgga taacaaactg gccgaactgg ccgaaaagat
     1261 ctatcaggaa agcattgaaa aatccaggtt ggatgcagaa gagatcatcc aaaatgcccg
     1321 tcatgaagcc gataatattc tgcgtgaagc aaaaaaccat aaagatgcct tgcttcatca
     1381 tgcagaacag gaagcagccg gctggaaggc aagagctttg gctgagatca gaatgcttac
     1441 agataatgct ttgactgctt tacgggaaga gataactcat atgatcaggt atcgggtcat
     1501 cgatgaaccg atccatcaga tccttgatca gaaagaacac gtagcagtac tgctgaccga
     1561 agccctcaaa catttgcatg aaggcgaaga cggacagatt caggttcagt tgcctgattc
     1621 ctttgaccag gaagctacag cctatctgca ggaggaagta ggaaggcaac tctctaaaga
     1681 accggaactg gggctgaccg gaggtcattt tcgtggattt gctattaaaa tgaaagggaa
     1741 aggatttacc atagacttca ctgaggaaag tttcaggtct ttgctgtccg cctatttcaa
     1801 tcaggaaatg aatgatctgc ttggaaatga agcctcccgt gaatcatgaa tactatggcc
     1861 tgatcacagg tttaccggat ctgactcttc aaaaaccgga tcttgccggt acaggaaata
     1921 tctgggcaca atatggtcat ttgctgacct cccatgatca gctgctgcta caacttgcct
     1981 acctgccggt tgatcataaa ctggttctgt gcagcttctt tgaacaaccc gtttctgaaa
     2041 ccgatcagct gctggcgtgc tacgacagtt ctgctcatca gtcttacctg cagcaaaacc
     2101 tgaaattatt tccctaccag gatcaaatgc tatttcccga caaagaatcg cccaggatat
     2161 cgctggaggc atcctggagg atcgcctgga caacctttgt tcgtc
//
LOCUS       DAOMAX010000382         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11993_length_2182_cov_7.177715, whole genome shotgun sequence.
ACCESSION   DAOMAX010000382 DAOMAX010000000
VERSION     DAOMAX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_2182_cov_7.177715"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..1708)
                     /locus_tag="PK742_13065"
     CDS             complement(356..1708)
                     /locus_tag="PK742_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77568.1"
                     /translation="MQGLCQESIVGNWRQAVGNSIKRVLQFFFGLQTENCQLSYFCSR
                     MNRNERILMVILAGINFTHILDFMIMMPLGPQLMRIFEISPAQFSTLVASYTFSAFAS
                     GLIAAFFVDRFDRKRVLLFGYTGFIIGTLGCAIAPTYLLLMLARIVAGLFGGLIGAQV
                     LSIIGDTFPYEKRATAMGALTAAFSLASVFGVPLGIYLATLFSWHAPFVLAGGLAIVI
                     NGLAMKFVPSISGHIQEKGANKAWVSYQHILADRNQQWAILLGVVMMLGHFSIIPFIA
                     TYLVQNVGFTEHEITYVYLLGGALTIFTSPMIGRIADRKGKFPVFALFCTLTLIPIFL
                     ITNMPPIAIGLALVVTSFFFIVVGGRMIPMQAMVTSVVPARQRGGFMSINSSIVQLGS
                     GTASMLAGLIISETPDGKLQNYNVVGYVAIAFSILAIFIARKLKPAEENSEVGMRKPE
                     "
     gene            1730..>2182
                     /locus_tag="PK742_13070"
     CDS             1730..>2182
                     /locus_tag="PK742_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77569.1"
                     /translation="MRVFYLTLIAAGLFLASCSEALSPYTTSVQRSANIGEEQVKQIQ
                     FYLSDDIVMQRQLSATETTITEGELKIVGGREVQEIVIPAGTPGVVTGLSGNILHVSF
                     DANGEYLRFGPNPGAGGRYTVMAYDKNGIYGYVMYGTTEFKLASYNNHY"
BASE COUNT          572 a          530 c          577 g          503 t
ORIGIN      
        1 ctggtttcag ctttcttgca atgaagatcg ccaggatact gaaggcgatg gctacatatc
       61 cgaccacatt atagttgtgt agtaagcagt cagcagtgaa ttaaaaggcg aagcaagtta
      121 gaaagatcgt tgcgtccttt gcgcttgttt cggcgaagcc ggaacctgtg cctttgcgtt
      181 aggcagtttt ctatacctta tcagttttca aattactgtc ccttgcgaag gaaatttgat
      241 caaaaaaaat gatatcgaaa actgagataa gagattcctg atagcttcct tcgtcagctt
      301 caggaatgac tccgctttat tgaatagaat atccaattcc aatttcagaa tgtgatcatt
      361 ccggtttccg cattccaacc tccgaatttt cctccgctgg tttcagcttt cttgcaatga
      421 agatcgccag gatactgaag gcgatggcta cgtagcccac tacattatag ttctgcagtt
      481 tgccatcggg tgtttctgag atgatgagtc ctgccagcat ggaagcagta ccactgccga
      541 gctgcacgat cgatgaattg atactcatga atcctccccg ctgacgggcc ggtaccactg
      601 aggtgaccat ggcctgcatc gggatcatcc gtccgcccac cacgatgaag aagaagctgg
      661 tcactacgag ggcaagtcct atggcaatgg gtggcatatt ggtgatgaga aagatcggga
      721 tcagggtcag ggtgcagaac aaagcgaaga caggaaattt tccttttctg tcggcgatgc
      781 ggcctatcat cggtgaagtg aaaatggtca atgcacctcc cagcaaataa acataggtga
      841 tctcatgttc ggtgaaacct acattctgca ccaaataagt agcgatgaaa gggatgatac
      901 tgaaatgtcc cagcatcatg accacgccca gcagaatagc ccattgctgg ttgcggtctg
      961 ccaggatatg ctggtaagaa acccaggctt tgtttgcacc tttttcctgg atgtgtccgc
     1021 tgatgctcgg cacgaacttc atcgccagcc cgttgatgac gatagcgagt cccccggcta
     1081 atacgaacgg tgcatgccag ctgaacaggg ttgccaggta gatgcccagt ggtacaccga
     1141 acaccgaagc cagcgagaag gcagcggtca gtgcacccat ggcagtggct cttttttcat
     1201 agggaaaggt atcaccgatg atgctcagca cctgtgcgcc aatgagtccg ccgaagagac
     1261 ctgcaacgat gcgggcgagc atcagcagca gataagtggg agcgatggca caacccaggg
     1321 taccgatgat gaatccggta tagccgaaca gcagaacccg ctttctgtcg aacctgtcaa
     1381 caaagaaggc tgcgatgagt ccggaagcaa aggcgctgaa agtatagctg gccaccaggg
     1441 tggagaattg cgccgggctg atctcaaaga tgcgcatgag ctgaggcccc agcggcatca
     1501 tgatcatgaa gtcgagaata tgggtgaagt tgatcccggc caggatcacc atcaggatgc
     1561 gttcgttgcg gttcatgcga gagcaaaagt aagatagttg gcagttctca gtctgtaagc
     1621 cgaaaaagaa ttgtaaaacc cgtttaatcg agttgccaac tgcctgccgc caattgccaa
     1681 cgatgctctc ttggcacagt ccttgcattc catctgtaaa atacaaggta tgcgagtatt
     1741 ctacctgaca ctgatcgccg ccggactttt cctggcttcc tgttctgagg cgctgagtcc
     1801 ttacaccact tcggtgcaga gaagcgccaa catcggagaa gagcaggtga aacagatcca
     1861 gttctatctg agcgatgaca tcgtgatgca aaggcagctg agcgctacgg agaccaccat
     1921 caccgaaggt gagctgaaga tcgtgggtgg tcgcgaagtg caggagatcg tcatcccggc
     1981 cggaactccc ggtgtggtaa ctggactttc aggcaacatc ctgcacgtga gtttcgatgc
     2041 caacggtgaa tacctcaggt tcggtccgaa ccccggtgcc ggtggtcgct ataccgtgat
     2101 ggcttacgat aaaaatggaa tttatggata tgtgatgtac ggaacaaccg agttcaaact
     2161 ggcatcgtac aacaaccact at
//
LOCUS       DAOMAX010000383         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_11995_length_2182_cov_5.364833, whole genome shotgun sequence.
ACCESSION   DAOMAX010000383 DAOMAX010000000
VERSION     DAOMAX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11995_length_2182_cov_5.364833"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /gene="rpoN"
                     /locus_tag="PK742_13075"
     CDS             complement(<1..1318)
                     /gene="rpoN"
                     /locus_tag="PK742_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQU77570.1"
                     /translation="MLNQKLSQKLLQKLSPQQIQLMKLLQIPTDQLEQRIKEELEANP
                     ALEESAEFEQETDDIFQDKEEKEEGEDSDTSDELDLSEYLDDDDYADYKTQGDSYNPD
                     DEEESRTIPIPVAESFHENLIKQLGMVQMDDRERAIARQLIGSIDDDGYLRREIAAIV
                     DDLAFSQNVMATEEEILELLDVVQGFDPPGVGARDLRECLLLQLERRLHTKTVNEAIT
                     VVDEYFEEFTKKHYEKLGKALKMDDEKLKEVIDEILKCNPKPGNTSKGDAALFNQYII
                     PDFTIVNNNGELELKLNSRNAPELRVSQEFREMMQDYKAGGKKDKKQKEAIMFIKQKI
                     DSAKWFIDAIRQRQQTLWNTMYHIMAHQYDFFLTGDEMNMKPMILKDIADVTGLDIST
                     VSRVANSKFVSTEFGTYPLKYFFSESLTTESGEEVSTREVKKLLDEI"
     gene            1440..>2182
                     /locus_tag="PK742_13080"
     CDS             1440..>2182
                     /locus_tag="PK742_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQU77571.1"
                     /translation="MNRIEPFQYISELKDHAGAQVELRGWVSNRRDSKGLVFIVLRDG
                     TGFAQCVVNEESVGAEQFEAAKRLGLESSVILWGEVREDERQIGGYEVQVSKLQVIQD
                     AADYPIAKKEHGVDFLIENRHLWLRSTRQWAIMRVRNRVIYAIHNFFQANGFLQMDAP
                     IFTGNAVEGTTTLFETDFFGQPAYLSQSGQLYGEALAMAHGKIYTFGPTFRAEKSKTR
                     RHLSEFWMIEPEMAFYDLDMDMDLIEQFI"
BASE COUNT          452 a          571 c          568 g          591 t
ORIGIN      
        1 tgatctcgtc cagcagcttc ttcacctcgc gggtactgac ctcttcacca ctctctgtgg
       61 tcagtgattc gctgaagaaa tacttgagcg gataagtacc gaactccgtg ctcacgaatt
      121 tgctgttggc tacccttgaa acagttgaaa tgtccagccc cgtcacgtcg gcgatgtctt
      181 tcaggatcat gggcttcatg ttcatctcat ctccggtcag gaagaaatca tactggtggg
      241 ccatgatatg gtacatggtg ttccacaagg tttgctgtcg ctgacggatg gcatcgatga
      301 accacttggc agaatcgatc ttctgcttga tgaacatgat ggcctctttc tgtttcttgt
      361 ctttcttgcc gccggcctta tagtcctgca tcatctcgcg gaactcctgg ctaacccgca
      421 attcaggtgc attcctggag ttgagtttca gctcgagttc gccgttgttg ttgacgatgg
      481 tgaagtcggg gatgatgtac tggttgaaca gcgctgcatc acccttcgag gtattacccg
      541 gtttgggatt gcacttcagg atctcatcga tcacttcctt cagcttctca tcatccatct
      601 tgagcgcctt gcccagtttc tcgtagtgct tcttggtgaa ctcttcaaag tattcatcca
      661 ctaccgtgat ggcttcattc accgttttgg tatgcaaccg tcgttccagt tgcagcaaca
      721 ggcactcacg cagatcgcgg gcacccacac ccggcggatc gaatccctgt accacgtcca
      781 gcagttcgag tatctcttct tcggtggcca tcacattctg cgagaaggcc aggtcatcga
      841 caatggcagc gatctctctt ctcagatatc catcatcgtc tatactgcca atgagttgtc
      901 tggcgatggc ccgttcccgg tcgtccatct gcaccatacc cagttgcttg atgaggttct
      961 catgaaaaga ctctgcaacc ggtatcggta tggtacggct ctcttcttcg tcgtcgggat
     1021 tgtaactgtc gccctgggtc ttgtagtcgg cgtaatcatc gtcgtccagg tactcgctca
     1081 ggtccagttc atcgctggta tcgctgtctt ccccctcttc cttctcttct ttgtcctgga
     1141 aaatgtcgtc ggtctcctgc tcgaattcag ccgattcttc cagggccgga ttggcctcca
     1201 gttcctcttt gatgcgctgt tcgagctgat cggtcggtat ctgcagcagc ttcataagct
     1261 gtatctgctg cggcgacagc ttttgcagga gtttctggct gagtttctgg ttgagcatta
     1321 cagtggtaaa ggtacgatgg gaatgtgaga tgcgaaacgg cagaagcccc gcaatgttgc
     1381 tgtttctgcc tgttttccct gcgctgaaat ccctaatttt gcagaccgaa atcataggta
     1441 tgaacagaat agaaccattt caatatatca gtgagctgaa agatcatgcg ggcgcacagg
     1501 tggagttgcg tggctgggtg agcaaccgcc gcgacagcaa aggcctggtg ttcatcgtgc
     1561 tgcgtgacgg caccggattc gcacagtgcg tggtcaatga ggagtcggtt ggggctgagc
     1621 agttcgaagc ggccaaacgt ttgggactgg agagctctgt cattttatgg ggagaagtgc
     1681 gggaagacga acgacagatc ggtggctatg aggtgcaggt gagcaagctg caggtcattc
     1741 aggatgcggc cgattatccc atcgccaaga aggaacacgg cgtggatttt ctcattgaga
     1801 accgtcatct ctggctgcgt tccaccaggc agtgggccat catgcgggtg cgcaaccgcg
     1861 tcatctatgc catccacaat ttctttcagg caaatggttt cctgcagatg gatgcgccca
     1921 tcttcaccgg taacgccgta gaaggaacca ctactttatt tgaaacagat ttcttcggac
     1981 agccggctta cctgtcgcag tccggtcagt tatatggtga agcgctcgcc atggcacatg
     2041 gcaagatcta caccttcggc ccgacttttc gtgcagaaaa atccaagacg agaagacacc
     2101 tcagcgagtt ctggatgata gaaccggaga tggctttcta tgacctcgat atggacatgg
     2161 acctcatcga gcagttcatc aa
//
LOCUS       DAOMAX010000384         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12066_length_2175_cov_4.266509, whole genome shotgun sequence.
ACCESSION   DAOMAX010000384 DAOMAX010000000
VERSION     DAOMAX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12066_length_2175_cov_4.266509"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..2124
                     /locus_tag="PK742_13085"
     CDS             178..2124
                     /locus_tag="PK742_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQU77572.1"
                     /translation="MSASSRLHKISAAGVLITLGIIYGDIGTSPIYVLRAIVGGHTIT
                     KDIVMGGMSCVFWTLIIITTFKYVYLALNNDNKGEGGIFALYALVKRYKIKWAIIPAL
                     LGCSALIADGFITPPISISSAIEGLLILNPEIPTIPIVVIILTALFVFQQFGTKVVGA
                     VFGPIMTIWFIMIGTLGAVELFKHPEVLEALNPWYGINLLVNYPGGFWLLGAVFLCTT
                     GAEAMYSDLGHCGKQNIRLGWSFVIFMLLLSYLGQSAWLMQHEGAFLDVDASPFYELM
                     PAWFLPVGIIVASMATIIASQAVITGCFTLVNEATKLKLWTNFKVVYPSTIKGQIYLP
                     AINWFLYAGCLTVIAIFKKSSNMEAAYGLTITIDMLMTTLLLATLLTLHRKRRITVTI
                     FLLIFLSMEGAFLASNLLKLSHGGWFTVMVATAIFFATWLFYQGRILRRKHIDYMELD
                     HFTGMLQDLMADDSVPKEATNLVFLARVDDKNHIDSSIYYSIFRKRPKRADVYWIVHV
                     EITNEPYGASYSVKTLIPQHAFLVRLQFGFKVEHKVNFMFRKIVTEMIENGEVDETSR
                     YASLKKHDVPADFKFILLNSRVASDDKLSPWQQFVIKGYRLIKKMGISRAEDFGLEQS
                     NLRVETVPISLGLTADIELERLDR"
BASE COUNT          504 a          600 c          493 g          578 t
ORIGIN      
        1 actcaccact cctcactcct cactgctcac tcctcactgc tcactcctca ctgctccctc
       61 ctcactcctc actcctcact gctcactccc cactgctccc tcatctgctc ctcactcatc
      121 aacaaccctc attagctaac tccctcattc cctatatttg tgcccgactc tttctccatg
      181 agtgcatctt ccaggttgca taagatctct gctgccggtg tcctgatcac cctgggcatc
      241 atatacggtg atataggtac gtcacccatc tatgtgttac gagccatagt tggcggacat
      301 accatcacca aagacatcgt gatgggcggc atgagttgtg tgttctggac cctcatcatc
      361 atcaccacgt tcaagtatgt ctatctggcc ctgaacaacg acaacaaagg ggaggggggg
      421 atatttgcac tctatgcact ggtgaaacgc tacaagatca aatgggccat cattcctgcc
      481 ttgctgggat gttctgcgct gatagccgat ggtttcatca ccccacctat ctctatttct
      541 tcggccattg aaggtctgct gatactcaat ccggaaatac caaccattcc cattgtggtc
      601 atcatcctga ccgcattgtt cgtatttcag caattcggta ccaaagtggt aggtgccgta
      661 ttcggtccta tcatgaccat ctggttcatc atgataggta ccctgggtgc tgttgaactc
      721 tttaagcatc cggaagttct ggaagcactc aatccctggt atggcatcaa cctgctggtc
      781 aactatcccg gtggattctg gttgctgggt gcggtttttc tctgtaccac cggagctgaa
      841 gccatgtatt cagacctcgg tcactgtggc aaacagaaca tcagactggg atggtctttc
      901 gtcatcttca tgttgttgct gagttatctt ggccagtcgg catggctcat gcagcacgaa
      961 ggtgctttcc tcgatgtaga tgccagtccg ttttatgaac tgatgccagc ctggtttctt
     1021 cctgtgggta tcatcgtggc atccatggct accatcatcg ccagccaggc agtgataacc
     1081 ggttgtttca ccctggtgaa tgaagctacc aaactgaagc tgtggacaaa ttttaaagta
     1141 gtgtatccca gcaccatcaa aggacagatc tatctgcctg ctatcaactg gttcctgtat
     1201 gccggctgtc tgacggtcat cgccatattc aaaaagtcca gcaatatgga agcagcatac
     1261 ggcctgacca ttaccataga tatgctgatg accaccctgc tgctggccac cctgcttacc
     1321 ctgcatcgca aacgcaggat cactgttacc atcttcctcc tcatcttctt gtctatggaa
     1381 ggtgcctttc tcgcatccaa tctgctgaaa ctttcgcacg ggggctggtt caccgtcatg
     1441 gtggcaactg ccatattctt cgccacctgg ctgttttatc agggacgaat cttacgccgt
     1501 aagcacattg actacatgga gctggatcat tttaccggta tgctgcaaga ccttatggca
     1561 gatgattctg tgcccaagga agctaccaat ctggtgttcc tggccagggt ggatgataaa
     1621 aaccacatag actccagcat ctactacagc atcttcagaa aacgaccgaa gcgggccgat
     1681 gtatattgga tcgtgcatgt ggagatcacc aatgaaccat atggtgcaag ctacagtgtg
     1741 aagacgctca taccgcagca tgccttcctc gtcagactcc agttcggatt caaggtagag
     1801 cataaggtca acttcatgtt ccggaagatc gtcacggaaa tgatcgagaa cggagaagtg
     1861 gatgagacca gccgctatgc atcgcttaag aaacacgatg tgcctgccga tttcaaattc
     1921 atcctgctga acagccgtgt ggccagtgat gataagctgt cgccctggca gcagttcgtc
     1981 attaaaggtt accgactgat caagaagatg ggcatcagtc gtgccgaaga cttcggcctg
     2041 gaacaatcca acctgcgggt ggaaacggtg cctatttcat tagggcttac agctgatatt
     2101 gagctggaaa ggctggacag ataactccct tcagaactgt ctctccgggc ttgacccgga
     2161 gtctccctca ttgat
//
LOCUS       DAOMAX010000385         2175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12067_length_2175_cov_4.127830, whole genome shotgun sequence.
ACCESSION   DAOMAX010000385 DAOMAX010000000
VERSION     DAOMAX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12067_length_2175_cov_4.127830"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1708
                     /locus_tag="PK742_13090"
     CDS             179..1708
                     /locus_tag="PK742_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77573.1"
                     /translation="MKNLQLLYQLVHRMTPDEHLYLEKLLLKDEGGMCRKLTNILLDM
                     SAYEENTVIYKWSRYGKKYSRKALYNTSHRTKDKILQAIHLSMAGAYPDSSDTIAKTA
                     DAYMQKQLFEEAYNILRSFLERQAGFISPVEELQFLHKMLSLAPLVVRTNLDTVHDDL
                     LQQCDKTLQHIQLYHRIHRLNHQVGLLYIKEANKKAQPEEIHTVLSAWEELSKIPSEH
                     HATHSYFLKTTVMFMQLRYAFEKQAEAQLELAGTMRSYYLLYKTSPHYLKFLAEQINA
                     GIILARSGDREAFEELIEFLQLVLQEEHVLHDLLRMQLWFIQSLFHYYRRDIQQMLQY
                     LAPAHHAFMEQRDKLPVTVQPALRLGLMKCWLLADQHRFIAKLFEERPAGKPLVKLDL
                     YLVMHLIYLCSLYLRFTYNVLSGVVTPPQGFMKQVNRFTSEYQLSKDFLPVEYSIHGT
                     FTQLAKADISQNHIRILQRLRKKLSKPEVAEARQTEAFLRTFSLDDWITDTIYKLEQL
                     K"
BASE COUNT          670 a          500 c          454 g          551 t
ORIGIN      
        1 aatgtggaaa tgtgagaatg agtaaatggg gaaatggggg ggcatatgac tctgtgtaaa
       61 tatcatacgg agtgcctcac tacccgatac tccataccca atacctaaaa cctaaatccg
      121 gcttctcagg atattgatca tgctatagtt acttatttct gttattttca ttatagctat
      181 gaaaaacctg caactgctct accagctggt tcacaggatg acacctgacg agcatttgta
      241 tctggaaaaa ctgctgctca aagacgaggg tggcatgtgc agaaagctga cgaacatttt
      301 acttgacatg tcggcctacg aggagaacac tgttatttat aaatggagca gatacgggaa
      361 aaaatattcc agaaaagccc tttacaatac tagtcaccga accaaggaca agatactaca
      421 ggccatacat ttgtccatgg ccggagctta tcctgacagt tccgatacca tagccaagac
      481 tgcggatgcc tacatgcaaa aacaattgtt tgaagaagca tacaatatat tgcgcagttt
      541 tctggaaaga caagcagggt tcatatctcc ggtggaagaa ctacagttcc tgcataaaat
      601 gctcagtctt gcgccacttg ttgtcaggac caatctggat actgtacatg atgatctgct
      661 tcagcaatgc gataaaacac tgcagcatat tcagttatac caccgcatac acaggctgaa
      721 ccaccaggtt gggttattgt atatcaagga ggcgaataaa aaagctcaac cagaagaaat
      781 acataccgtg ctatcagcct gggaagaact cagcaaaatt ccttctgagc accatgctac
      841 gcacagttat tttctcaaga ccacggtgat gttcatgcaa ctgaggtatg catttgaaaa
      901 acaggcagaa gctcagctgg agttagcagg taccatgcga tcatactatc tgctttataa
      961 aacctctcca cactacctga agtttctggc agaacagatc aatgccggaa tcatattagc
     1021 aagatcagga gacagggaag catttgaaga actgatagaa tttctgcaac tggtactaca
     1081 ggaagaacat gtattacatg atctgctgcg catgcaactg tggttcattc agtcattatt
     1141 tcactattac cgcagagaca tacagcagat gttacaatac ctggcacctg cacaccatgc
     1201 attcatggag caaagagaca agctacccgt taccgttcag ccagcactcc gattgggact
     1261 catgaagtgc tggttgctgg cagaccagca ccgcttcatc gcaaagcttt ttgaagagcg
     1321 tcctgcaggt aaaccgttgg tcaagctgga cctgtatctg gtcatgcacc tgatctatct
     1381 ctgttcgctc tatttgcgat tcacctacaa tgttctatca ggtgtggtga caccaccaca
     1441 gggtttcatg aagcaggtaa acagatttac cagtgagtat cagcttagca aagatttcct
     1501 tcctgtagaa tacagcatac atggcacttt tacacagtta gcaaaagctg acatatctca
     1561 aaaccacatt cgcatactac agaggctgag aaaaaagctc agcaaaccgg aagtagcaga
     1621 ggccaggcag acggaagcat ttctgcgcac cttttcgtta gatgactgga taactgacac
     1681 catttacaaa ctcgaacagt tgaaataaaa aaccccgctt acgcggggtt gctgttaagt
     1741 acagcggcag gctcatggat tgagcatatc ccagatccac tccaggacct cgatccaaac
     1801 agttggttcg ttcataaaac ctcctcgcta tgcccgggtg gtcttcctcc gactgatcgc
     1861 cacccggccg ttactaagac cggagctgct caggcagctt agcgccttgc aggactgcag
     1921 ttcacgattg cagctttttg caaggtatcg ggaggctaag gggctgagaa ttggtttaca
     1981 accaaaccgt cagataatac aaccaaacca aaaaacagat gtcaactata tacctaaaaa
     2041 ttccttcagc tgtttcatta aatctttacc aagcttcaaa ctagtttttt catcaaagat
     2101 tacatatgta tcatctttgg taatagcaag gatattttcc gtattaataa tgcatttctt
     2161 ttcaaagaaa ataaa
//
LOCUS       DAOMAX010000386         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12084_length_2173_cov_3.624174, whole genome shotgun sequence.
ACCESSION   DAOMAX010000386 DAOMAX010000000
VERSION     DAOMAX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12084_length_2173_cov_3.624174"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1214
                     /locus_tag="PK742_13095"
     CDS             168..1214
                     /locus_tag="PK742_13095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77574.1"
                     /translation="MILTKARRACCLLYAGFCAWIPLSVTAQSPAMQYELHYVEKTET
                     YFFRTDLDWEPNQLELLRMMPYTVKDSVIKQVTTDNDIKTTIWHLENTQLEEWMDQPS
                     RTVIDNIRTRSYDAGNNLMLNLPHTATYLDSYNQLKDKMTLTGGDIIPGFPVLTNKLK
                     QDFLFNGFSMTNLGGGAVSFSKDSLTVVFNNKKKVLETKISDAEGAIIHYEKKGFAKN
                     NLNQLVPAYELVRSMDTRYPQGLVYKVELTTYPEYSVNYISPLREAQGKDAISWNLYP
                     NPAQDQIIIEWMNENGAEASWQIISLAGIEMASGQVDSDRLLELDISKFPSGCYIMIL
                     RSGGQIERDQFIKL"
     gene            1225..>2173
                     /locus_tag="PK742_13100"
     CDS             1225..>2173
                     /locus_tag="PK742_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77575.1"
                     /translation="MKKIFIQIFAYISPVLTLAQIVSPDGGPVATFGGGTLTVSEVDP
                     GPEIIDTWEPPVMEGDRSVIWIHGLGGHGDLSASDESNSWIQASSTSDINYQMESRRP
                     DYSDVSLDAASTTLRTQLEDYDLSTDAFLIAHSQGGIVSRQFDKYIHDLGWERTFNGV
                     VTFGTPHGGAMILNNRDLIVQWLDEACDDLGNGPWQETLDDSFFFSILNLFSGIENAD
                     DKVCALLIDEILPVMFNLDAYYAGVTDGYYVGSPELDELNAYTPDIPYVTFWGEETEP
                     VSWNTMVHMMPGRSVNSEIYGPFGANNDSYLTDQMIWLQN"
BASE COUNT          624 a          463 c          512 g          574 t
ORIGIN      
        1 atatagtata tactatatat aatattaaat acggaataca aatctaattc aaaaattatt
       61 tttttcacaa ttgataaaaa atagtggaaa aaataaagtg aaaatatttt gatagttcgc
      121 tgagacgcct acttttgtct tcattcttaa ccctcaaaat gcttgatatg atcttgacga
      181 aagcgaggcg ggcatgctgt ctcttgtatg caggattttg tgcctggata ccattatctg
      241 taacagcaca atctcctgcc atgcagtatg aactgcatta tgtagaaaaa acagaaacgt
      301 attttttcag aactgacctg gactgggagc ccaaccagct ggagctgttg cgtatgatgc
      361 cctatactgt aaaagatagt gtcatcaagc aggtaaccac agacaatgac atcaaaacca
      421 cgatctggca tctggagaat acccagctgg aagagtggat ggatcagcct tccagaacgg
      481 tgatcgacaa catccgcacc agatcctatg atgccggtaa taacctgatg ttaaacttgc
      541 ctcatacagc tacatatctg gatagctata accagttgaa agataagatg actctgacag
      601 gaggtgacat catacctggg tttcctgtat tgaccaacaa gctaaaacag gattttcttt
      661 tcaatggatt cagtatgaca aacctgggtg gaggagctgt atccttcagc aaggatagcc
      721 tgacggtagt gttcaacaac aagaaaaaag tgctggaaac aaaaatttct gacgctgaag
      781 gcgctatcat tcactacgaa aagaaaggat ttgccaagaa taatctgaat caattggttc
      841 cggcttatga gctcgtgcga tccatggata ccaggtatcc gcagggattg gtgtataaag
      901 tggagttgac cacctatccg gagtattccg tcaactatat ctcacctctc agagaagccc
      961 agggcaaaga tgccatatcc tggaatctgt atcccaaccc ggcacaggac cagatcatca
     1021 ttgaatggat gaatgagaat ggtgctgagg caagctggca gatcattagt ctggcaggca
     1081 ttgaaatggc ttcgggccag gtggatagtg accggttgct ggaactggat atcagcaaat
     1141 ttccctcagg ttgctatatc atgatcctgc gttccggtgg tcagatagag agagatcagt
     1201 tcattaaact gtaaaaaaac gactatgaaa aagattttca ttcaaatatt tgcatatatt
     1261 agccccgtgc ttacccttgc tcaaattgtc agccctgacg gcgggcctgt agccacattt
     1321 ggtggaggta ccctgaccgt ttctgaagtg gatcccggcc ctgagatcat tgatacctgg
     1381 gagcctcctg taatggaagg agacaggtcg gtcatctgga tacacggtct gggtggtcac
     1441 ggtgatctgt cagccagtga tgagtctaac agctggatcc aggcttccag cacctcagac
     1501 atcaattacc agatggaatc acgcaggccc gactattctg atgtaagcct ggacgctgca
     1561 agcactaccc tgcgaaccca gttggaagac tacgacttat ctacagatgc tttcctgata
     1621 gcacacagcc agggcggtat tgtaagccgg cagtttgaca agtatatcca cgacctgggt
     1681 tgggaaagga ccttcaacgg cgtggtaacc tttggtacac cccatggtgg tgccatgatc
     1741 ctgaacaacc gcgatctgat cgtacaatgg ttggatgaag cttgtgatga tcttggaaat
     1801 ggcccctggc aggagacttt ggatgactct tttttctttt ctatcctgaa cttgttttct
     1861 ggaatagaga atgctgatga taaagtctgt gccctgctga tagatgaaat tctgccagtt
     1921 atgttcaacc ttgatgccta ctatgcgggt gtgaccgatg gctattatgt agggtcgccg
     1981 gagttggatg aactcaacgc atatacacct gacattccgt atgtgacatt ctggggtgaa
     2041 gaaacagagc cggtttcctg gaacacaatg gtccacatga tgcccggccg aagtgtaaat
     2101 tctgaaattt atggtccttt tggggcaaac aatgactctt atctaactga tcaaatgatt
     2161 tggcttcaga ata
//
LOCUS       DAOMAX010000387         2137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12412_length_2137_cov_3.927954, whole genome shotgun sequence.
ACCESSION   DAOMAX010000387 DAOMAX010000000
VERSION     DAOMAX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12412_length_2137_cov_3.927954"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PK742_13105"
     CDS             complement(<1..1147)
                     /locus_tag="PK742_13105"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HQU77576.1"
                     /translation="MKKILLPVILLSAFTAGAQVSIVGSGVPVSEDFTGFDGTGFDPA
                     PAAGQLNSNNWEVLGFSDGDLLFGGSAATGDLARGITTGNITSGGLYAFDNSGNTAVY
                     IQPTSSDFNPGSITWKLQNNTGSILTEVDLAYTVYIYNNEGRANNWNCSYSYDNTTFV
                     NIPSLDLVSVEADEDLLYTYNQSTSIPGLSIGDGDFFYIRWDGADVSGSGSRDEFGLD
                     DISLTAYAGEVTPLAELVEDVSVVEGAGAATVSVELNVSANCDVYVSATDGTAEMFVD
                     YLFDPAVISFTEGGATMQTFDVLIGNDFDLEFTETLTVHIDSTSEGCATGLSNEVTVS
                     IIDDETAATGPCENLFFSEYIEGSSNNKALEIYNPGAVDIDMDGYVVK"
     gene            complement(1200..1628)
                     /locus_tag="PK742_13110"
     CDS             complement(1200..1628)
                     /locus_tag="PK742_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQU77577.1"
                     /translation="MTRTTWSFPNPVHFTTQLEVRIGDINYGGHLGNDKVLSLAQEAR
                     VQMLRSMGYSELDVEGPGIIMNEAEIKYVKEAFAGDILTVDLHVGNFSAVGCSIYYNI
                     RIMDKVIAVVRTGIVFYDYNSRKVVRVPSVFQQRFNDNLA"
     gene            complement(1625..>2137)
                     /locus_tag="PK742_13115"
     CDS             complement(1625..>2137)
                     /locus_tag="PK742_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQU77578.1"
                     /translation="AKMLGLPYPGGPQIDALASQGDPGTFRFADPSVPGLDFSFSGLK
                     TSILYTIRDAVKKEPDFIAAHLPDLCASVQHTINNILLKRVAKACMETGIREVALAGG
                     VSANSGLRTMMQTAAKEHGWNLYIPPLSYCTDNAAMIAMTGYLAVQRGGVGSLDDSPQ
                     ARMEFIELQV"
BASE COUNT          585 a          542 c          449 g          561 t
ORIGIN      
        1 ctttgactac atagccatcc atgtctatgt caactgctcc ggggttatat atctccaggg
       61 ccttattatt ggaagaacct tcaatatatt cactaaagaa cagattttca cacgggcctg
      121 ttgcagccgt ttcgtcgtct atgatagaaa cagttacttc attgctcaat cctgttgcgc
      181 aaccttcact ggtactgtca atgtgcacag taagcgtttc tgtaaactcc aggtcaaagt
      241 catttccaat gagcacatca aatgtctgca tggttgcacc gccttcggtg aagctgatca
      301 cagcgggatc gaaaaggtaa tcaacaaaca tttcggctgt tccatcggta gcagaaacat
      361 aaacatcaca gttggctgaa acattcagct caacagaaac agtggcggca ccggcacctt
      421 ccacaacaga aacatcctcc accagttcag caagaggtgt tacttcacct gcataagctg
      481 tcaggctgat atcatccaga ccaaattcat ctcttgatcc ggaaccacta acatctgcac
      541 catcccatct gatgtagaaa aaatcaccat caccaatact cagacctgga atactggtgc
      601 tttgattgta agtgtaaagc aggtcttcgt cggcttctac actcaccaga tccagcgacg
      661 gaatattgac aaatgtggta ttgtcataag aataagaaca gttccaattg tttgcacggc
      721 cctcattgtt gtagatatat acggtatagg ccaggtccac ttctgtcagt atgctgcctg
      781 tattattctg cagcttccag gtgatactgc ccggattgaa atcagagcta gtaggttgta
      841 tatacacagc tgtgtttccg gaattatcaa atgcatacaa gccaccggaa gtaatgttac
      901 cagtggtgat tccacgagcc agatcgcctg tggcagctga acctccaaac aacaggtctc
      961 catctgaaaa tccgagcact tcccagttgt tggaatttaa ttgtccggca gcgggtgcag
     1021 gatcgaaccc ggttccgtca aaaccggtga aatcttctga cacaggcaca ccggaaccca
     1081 caatagatac ctgggcgccg gcagtaaacg cagataataa gataacaggt aataagattt
     1141 ttttcatgat caggaatttg ctgtaaagct acctgatcaa atgcaacagt acttggattc
     1201 tatgctaaat tatcattaaa ccgttgctga aataccgaag gaacccgcac cacttttctg
     1261 ctgttgtaat cgtaaaaaac aataccggtg cgtactacag caattacctt gtccattatt
     1321 cttatattat aatatatact acatccaact gctgaaaagt ttcccacatg caggtcaaca
     1381 gttagaatat cgcctgcaaa agcctctttg acatacttga tctctgcttc attcatgatg
     1441 ataccaggcc cttctacatc cagttcagag tatcccatag accgaagcat ctgcaccctc
     1501 gcctcctgag ccagggaaag aactttatca ttacccagat gacctccata gttgatatcg
     1561 ccaatacgca cttcgagctg ggtggtaaag tgtaccgggt tcggaaaact ccatgttgtt
     1621 ctggtcatac ctgcagttct atgaattcca ttctggcctg ggggctgtca tccaaactcc
     1681 ccacccctcc acgctgaact gccagatatc cggtcatggc gatcatggcg gcgttgtcag
     1741 tgcaataaga aagtggtggg atatacagat tccacccgtg ttctttggct gctgtctgca
     1801 tcatggttcg taaaccgctg ttggcagaaa ccccgccagc cagtgccact tcacgaattc
     1861 ctgtttccat acatgccttt gcaactcttt tcagcaatat attgttgatg gtatgttgca
     1921 cggaggcgca gagatcaggc agatgtgctg caataaagtc aggctccttc ttcacggcat
     1981 cacgtatcgt gtacaggata ctggttttca gtccgctgaa agaaaaatcc aatccgggca
     2041 ctgatggatc cgcgaaacgg aatgttcccg gatctccctg tgatgccaga gcatcaatct
     2101 gcggtccgcc gggataaggg agccccagca tcttggc
//
LOCUS       DAOMAX010000388         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12629_length_2115_cov_4.478155, whole genome shotgun sequence.
ACCESSION   DAOMAX010000388 DAOMAX010000000
VERSION     DAOMAX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12629_length_2115_cov_4.478155"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PK742_13120"
     CDS             <1..1468
                     /locus_tag="PK742_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77579.1"
                     /translation="ALRIFAAVPAMSPGRILLFLVIVALSLTAIALLFPQDGIHLGKQ
                     LELRFVSKEELLAQKEKVVVDITDAQHLADSLEAIESFPDAGQVEEQIIPDPGSIKQR
                     LEWGSNGRASLNTFFAHLDSLTLTGGSMRIMHYGDSQIEADRMTSYLRDKFQRYFGGR
                     GPGLVPVISFSNPAGIKVTTQGNWEQYGLFGNLSGKGMTFGPLFSFASMCKQPADSAM
                     LDTTLSASAAFQAGSALYSNGRSFNRVRVFYSNSDPDATITLEAGGEQMVRSLDTSHT
                     MFTMPLGSFANTLSFTVQGNASTHIQGVSLESQSGVVVDNIPLRGSSGTIFTQGSRRM
                     LADAYRAMGVDMFILQFGGNVMPYIEDEQACINYGNWFASQIKYLKSIRPDAVFIVIG
                     PSDMSTKIGTEYVTYPLLPNVRDALRKAALQSGAAYFDLFEAMGGENSMQAWVNADPP
                     LAAPDYTHFSSRGARLMGEMFYEALMSEYHQYKSDNGR"
     gene            1514..>2115
                     /locus_tag="PK742_13125"
     CDS             1514..>2115
                     /locus_tag="PK742_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77580.1"
                     /translation="MTSATFAQIDTSRYTYIQYRLNRLDNPDSSYALAGFWEQFDAMR
                     VYGDRQLNIVHFGGSHIQADIYPNVLRNTFHTLDSGRTGARGFLFPYSAIHTNNPWNY
                     KTSYTGTWEGHRSAVYKHSMTWGVDGVTATTRDSVASLSFSFRDSTVAVRPVSVLVMA
                     DLSDSSYRLETDSIPHLLLTDTTSEGYLLLVYDGPVDTVFL"
BASE COUNT          498 a          542 c          549 g          526 t
ORIGIN      
        1 tgctcttcgt attttcgcag ccgtgcccgc catgagtccc ggtagaattt tgttgttcct
       61 ggtcatcgtc gccctgtcgc tgactgccat tgccctgctg tttccgcagg atggcatcca
      121 tctgggcaaa caactggaac tgcgttttgt atcgaaagag gaactcctgg cgcaaaagga
      181 aaaagtggtg gtggatatta ccgacgccca gcacctggca gattcgctcg aagccataga
      241 gtctttcccc gatgcaggtc aggtagaaga gcagatcatc cctgatcccg gatccatcaa
      301 acagcgactg gagtggggca gcaacgggag agcttcattg aatacttttt tcgcccacct
      361 ggacagcctg acacttacgg gtggcagcat gcgcatcatg cactacggag attcccagat
      421 agaggcagat cgtatgacat cttacctgag ggataaattc cagaggtact ttggtggcag
      481 gggacccgga cttgtcccgg tcatcagctt cagcaatccc gccggaatca aggtgaccac
      541 ccagggcaac tgggaacaat acggtctgtt cgggaatctg agtggtaagg gcatgacctt
      601 tggtccgctg ttctctttcg cctccatgtg taaacaacct gcagattcag ccatgctgga
      661 tactactttg agcgcttctg ctgcatttca ggcaggctca gcactgtaca gcaatggcag
      721 aagttttaac cgggtcaggg tattctacag caacagcgat ccggatgcca ccatcaccct
      781 cgaagccggt ggcgaacaga tggtgcgcag cctcgatacc agccatacca tgttcaccat
      841 gccactgggt agtttcgcca ataccctgtc attcaccgtg cagggaaatg cgagtaccca
      901 tattcaggga gtcagcctgg aatcacaatc aggtgtggtg gtagataata ttccccttcg
      961 gggaagttcc ggtaccatct tcacccaggg aagcaggcgc atgctggcag atgcctatcg
     1021 tgccatgggg gtagatatgt tcatcctgca gttcggtggc aatgtgatgc cttatataga
     1081 agatgaacag gcatgcatca actatggcaa ctggttcgca tcacagataa aatatctgaa
     1141 aagtatccgg cccgatgcgg tattcatcgt catcggaccc agcgatatgt ccaccaagat
     1201 cggtacggag tatgtgacct atccgttgtt acccaatgta agggatgcac ttcgaaaagc
     1261 agccttgcaa tccggtgcag catactttga tctgttcgag gccatgggtg gagagaattc
     1321 catgcaggcc tgggtcaatg ccgatcctcc gctggcagcg ccagactaca cccatttttc
     1381 atcacgtggt gccagactca tgggcgaaat gttctatgag gccctgatgt ctgaatacca
     1441 ccaatataaa agcgacaacg gtcgttgatc gattatgtgc agcaggacca ttttcttctt
     1501 gtttttatta gggttgacaa gtgcaacttt tgcacagatc gataccagcc gctataccta
     1561 tatacagtac cggctcaatc ggctggacaa tcctgacagc agttatgccc ttgccggatt
     1621 ctgggagcag ttcgatgcga tgcgggtata tggcgacaga caactcaaca tcgtgcactt
     1681 cggtggatcg catatccagg cagatatcta tccgaatgtg ttgcgcaaca cctttcatac
     1741 cctggacagc ggcaggacag gagctcgtgg ttttctgttt ccgtattcgg caattcatac
     1801 caataatccc tggaactata agaccagcta taccggaacc tgggaagggc accgaagtgc
     1861 cgtatataaa cacagcatga cctggggggt ggatggtgtg accgctacca cccgcgattc
     1921 tgttgcatca ttgtcttttt ctttccgcga ttcaactgtg gctgtgaggc ctgtttctgt
     1981 gctggtgatg gcagatctga gcgactcttc ctatcggctt gaaacagata gtataccgca
     2041 tctcctgctt actgacacca catcggaagg atatctgttg ctggtgtatg atggtccggt
     2101 tgatacggtc tttct
//
LOCUS       DAOMAX010000389         2091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12898_length_2091_cov_4.014735, whole genome shotgun sequence.
ACCESSION   DAOMAX010000389 DAOMAX010000000
VERSION     DAOMAX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12898_length_2091_cov_4.014735"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PK742_13130"
     CDS             complement(<1..1100)
                     /locus_tag="PK742_13130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQU77581.1"
                     /translation="MITYRTEKDTMGTVQVPADAYYGAQTQRSIMNFPIAQDINRMPK
                     EIIRAFAYLKKAAAITNNELGVLDDVKCKLIGEVCDEILAGKLDDQFPLVVWQTGSGT
                     QSNMNVNEVVAYRAHVLNGGSLTDEKKSVHPNDDVNKSQSSNDTFPTAMHIAAYKILM
                     EVTIPGIETLRDTLKQKSEAYMDVVKIGRTHLMDATPLTLGQELSGYVAQLDHGLRAI
                     RNSLTHLSELALGGTAVGTGINTPTGYAVKVAENIARLTGLPFVTAPNKFEALAAHDG
                     IVEAHGALKMVAVSLMKIANDIRLLASGPRCGIGEIHIPDNEPGSSIMPGKVNPTQCE
                     AMTMVAAQVMGNDVAINIGGSNGHFELNVFKPV"
     gene            complement(1243..1599)
                     /locus_tag="PK742_13135"
     CDS             complement(1243..1599)
                     /locus_tag="PK742_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQU77582.1"
                     /translation="MFLNLAHTKLDVYRSTRRFVMDCYRLTADLPEEEKYNLSMQIRR
                     AALSVHLNLAEGASRKSISERKRYFEISRGSVVEVDAALDIADDLYSLNHELLITLEQ
                     SMKSTFRGLSGLIRTI"
     gene            complement(1726..>2091)
                     /locus_tag="PK742_13140"
     CDS             complement(1726..>2091)
                     /locus_tag="PK742_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQU77583.1"
                     /translation="MGQSTCIGIGGDPVIGTTTLDAVKLLMADDDTEGIILIGEIGGT
                     MEAQAAQWIKENGTKPVVGFIAGQTAPKGRTMGHAGAIVGGKDDTAAAKMEIMRACGI
                     HVAETPAVLGEKMREAIAR"
BASE COUNT          462 a          547 c          498 g          584 t
ORIGIN      
        1 actggcttga acacattgag ttcgaagtga ccgttggaac ctccgatgtt gatggccacg
       61 tcattgccca tcacctgggc agccaccatg gtcatggcct cacactgggt gggattgacc
      121 ttgccgggca tgatggaaga accgggttcg ttatcgggta tgtggatctc gccgataccg
      181 cacctcggac cactggccag cagcctgatg tcattagcga tcttcatcag actcacagcc
      241 accatcttca atgctccgtg tgcttccacg atgccatcgt gtgctgccag tgcttcaaac
      301 ttgttgggag cagtcacgaa gggcaggcct gtcagcctgg cgatgttctc agccactttt
      361 acggcatacc ctgtaggtgt attgataccg gttcctacag ctgtgcctcc caatgccagt
      421 tcgctcagat gggtgaggct gttgcggata gctcgcaacc cgtgatccag ttgtgctaca
      481 tatccgctca actcttgtcc gagggtaagg ggagtggcgt ccatcaggtg cgtacgaccg
      541 atcttcacca catccatgta ggcttctgat ttttgtttca gtgtatccct gagggtctcg
      601 atgccgggga tggtcacctc cattaatatc ttataggcag caatgtgcat ggcagtaggg
      661 aaagtgtcat tgctgctctg cgatttgttg acgtcatcgt tcgggtgaac ggacttcttc
      721 tcatctgtga gtgagcctcc gttgagcaca tgtgcccggt atgccaccac ttcattcacg
      781 ttcatattgc tctgcgtgcc ggagccggtc tgccagacca ccagcggaaa ctgatcatcc
      841 agttttcctg ccaggatctc atcgcatact tcgccgatga gtttacactt gacatcatcg
      901 agcacaccca gctcattgtt ggtgatggca gctgcttttt tcagataagc gaaagcacgg
      961 atgatctcct tcggcatcct gttgatgtcc tgcgcaatgg gaaaattcat gatgctgcgc
     1021 tgggtctgtg cgccgtagta tgcatcagca ggaacctgaa cagttcccat ggtgtctttt
     1081 tcggttctat aggtaatcat cagtaaagta taatttagtt atagattgaa atgtgataag
     1141 tgagcggtga atcccgttta tctccgatta acatcgggat aaggcgggaa gtgaacccga
     1201 tcaggatcag gagtgaatgg ttgctcactt tggatttgtt cttcatattg ttctgatcag
     1261 tccgcttaaa ccacgaaaag tacttttcat ggactgttca agggttatca ataattcatg
     1321 attcagagaa tacagatcat cggcaatgtc cagagcagca tcgacctcta ccacacttcc
     1381 tcttgagatc tcgaagtatc ttttgcgttc ggatatggat tttcgtgatg ctccttccgc
     1441 aagattaagg tgaacagata gcgcagctct tctgatctgc atggagaggt tgtatttctc
     1501 ttcttccgga agatctgccg tcaatcggta gcagtccata acaaatcttc ttgtggatct
     1561 atatacatcc aatttggtat gagctaagtt caaaaacatg gttaagcatt tgatgagctc
     1621 caatgttaat gaaaaaaata tctatttaat aaaaaatata aatcatcttg tcaggatctc
     1681 acccctcact tcctgcattc gcaggattca cccatcacgc acgcttcagc gtgcaatcgc
     1741 ttcccgcatc ttctcgccca gcactgccgg tgtttcggcc acatgaatgc cgcaggcccg
     1801 catgatctcc atcttcgcag cagcagtatc gtccttgcca cctacaatgg cgccggcgtg
     1861 acccatggtt cttcctttcg gtgccgtttg tccggcaatg aaacccacca ccggtttggt
     1921 gccgttctct ttgatccact gagcagcctg cgcttccatg gtaccaccga tctcgccgat
     1981 caggatgatc ccttcggtat cgtcgtcggc catcagcaac ttcactgcat cgagggtagt
     2041 cgttccaatt acaggatctc cgccaatacc tatgcaggtg ctttgtccca t
//
LOCUS       DAOMAX010000390         2087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_12948_length_2087_cov_4.184055, whole genome shotgun sequence.
ACCESSION   DAOMAX010000390 DAOMAX010000000
VERSION     DAOMAX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12948_length_2087_cov_4.184055"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK742_13145"
     CDS             complement(<1..215)
                     /locus_tag="PK742_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77584.1"
                     /translation="MPIRKWAVWIILAAFVEIPHTLQAQLEVTTGYTATELANALAGT
                     GVTISGASLVCANGASGMFDCLDCNVG"
     gene            complement(278..1336)
                     /locus_tag="PK742_13150"
     CDS             complement(278..1336)
                     /locus_tag="PK742_13150"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HQU77585.1"
                     /translation="MNRSGKLFLFLLTITGVVSCKQVDSTNLPYSIGTVDEMVVVLNS
                     VDSTDTLFRVIDEYLGASYGYTPQPERRFNVIYADMATYQNTFERHRNLVFIGDLEQP
                     TAFSRFIGELVGEDRVAKLKQAGNSFYLPLENMWSHPQYVEAIVAADRDLLLADLDNI
                     LTGLLQRVSDRELNKIRSNQYVAGHNLQVEEALADAGINLQVPADFKALSVSKENFWF
                     LRKSTIDMTASIMIYWEPYSDSAQLKPEYVLHLRDSLGLTYESSKVANSFMQTEYRVA
                     QLHEQIDFNGAYALRSRGLWRLVNDFMGGPYINFWVYDEQHNRMVYLDGYVYAPDMRK
                     RPLVRQLEAILTSYDPVP"
     gene            complement(1333..>2087)
                     /locus_tag="PK742_13155"
     CDS             complement(1333..>2087)
                     /locus_tag="PK742_13155"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQU77586.1"
                     /translation="ILPAEFTYHYDMVDTVMVTRKMTLDQIGPYVGMTVDELKYYNPC
                     LKTNTVPGGNTPYSLRLPNEAVAMWITNEEQLYASLETPVKQETATVSRSEKEPVAAV
                     TTAVAEKKEVASTVTVSYVVKKNDNLGYISQWFNCSVSDLKKWNHLSSTKIMPGQKLK
                     IVVPADQQEHYASVTHMSFSDKQKLSDMQMVQSGEQKKESEVVYYTIKPGDSLWSISK
                     KYPENTIATLQEMNQIGQNETLKVGSKIKIVK"
BASE COUNT          492 a          495 c          495 g          605 t
ORIGIN      
        1 aagcccacat tgcaatctag acaatcgaac atgccgcttg caccgttggc acaaacaagg
       61 gaagccccac taatggtaac acctgttcct gccagtgcat tggccaattc tgttgcggta
      121 tatccggtag tgacctctaa ctgagcctgc agggtgtggg gtatttctac aaatgctgcc
      181 aggatgatcc agacagccca cttcctaatc ggcataattc ttcaggttcc gttcttagat
      241 aacagaacct aaatgtaatt aaattgtcaa ataatctcta aggaaccggg tcatagctgg
      301 tgagaatagc ttcgagctgt ctgaccagcg gacgtttacg catatccggt gcatagacat
      361 atccgtccag gtacaccatc cggttgtgct gttcatcata gacccagaag ttgatatagg
      421 ggccacccat gaagtcattg accagccgcc acaaacccct tgatcgcaat gcataggcgc
      481 cgttgaaatc tatttgttca tgcagctgcg ctacgcggta ttcggtttgc ataaagctat
      541 tggcaacctt gctgctttcg taggtcagac cgaggctgtc acggaggtgt aatacatatt
      601 caggtttcag ttgtgcagaa tctgagtagg gttcccagta gatcatgata ctggcggtca
      661 tatcaatggt actctttcgc agaaaccaga agttctcttt tgacacagaa agggctttaa
      721 agtcggcagg aacctgcagg ttgatgcctg cgtctgccag agcttcttcc acttgcaggt
      781 tgtgtccagc cacatactgg ttacttctga tcttgttcag ctcacgatca gaaacccgct
      841 gtaacagtcc ggtcagaata ttgtccaggt ctgccagcag caggtcacgg tctgcagcta
      901 cgatggcttc tacatactgc gggtgtgacc acatattctc cagcggcaga taaaaactat
      961 tgcctgcctg tttgagtttg gctacgcggt cttcgcccac aagttcgccg atgaagcgtg
     1021 aaaatgcagt gggttgttcc agatcgccga tgaacaccag gttgcgatgc cgttcaaagg
     1081 tgttctggta ggtggccatg tctgcatata tgacattgaa gcgccgttcg ggttgtggtg
     1141 tatatccata gctggcaccg aggtattcat ctatgacacg aaacagggta tctgttgaat
     1201 ctacgctgtt aagtaccact accatttcat ctaccgtgcc tattgagtaa gggaggttgg
     1261 tagaatctac ctgcttgcag gatactacac ctgtgatagt aagaagaaac aggaataatt
     1321 ttcctgaacg attcatttta cgatcttgat tttcgaaccc accttcaggg tttcgttctg
     1381 tccgatctga ttcatttcct gcagtgttgc gatcgtattt tccggatact ttttagagat
     1441 agaccacagt gaatcgcctg gtttgatggt gtaatacacc acttcagatt cttttttctg
     1501 ttcgccgctc tgtaccatct gcatgtctga cagtttctgt ttatcactga agctcatgtg
     1561 ggtaacagat gcatagtgtt cctgctgatc tgccggcaca acgatcttta gtttctgacc
     1621 gggcatgatc ttcgtggagc tgaggtgatt ccattttttg agatccgaca cactgcagtt
     1681 gaaccactgg ctgatatatc cgaggttgtc gttcttcttt actacatagc tcactgtaac
     1741 agtggaagca acttctttct tttcggcaac tgcagtagta actgctgcaa ccggttcttt
     1801 ttctgatctg ctgaccgttg ctgtttcctg tttcaccgga gtttccagtg aagcatacaa
     1861 ttgttcttca ttggtgatcc acatggctac cgcttcattg ggcagacgca gggagtaagg
     1921 tgtattgcca cccggaacgg tattggtttt caggcagggg ttgtaatatt tcagttcatc
     1981 tacagtcatg cccacatacg gaccgatctg atccagtgtc atcttgcggg taaccatcac
     2041 tgtgtccacc atatcatagt gataggtaaa ttcagccggc aggatgt
//
LOCUS       DAOMAX010000391         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13078_length_2076_cov_2.261752, whole genome shotgun sequence.
ACCESSION   DAOMAX010000391 DAOMAX010000000
VERSION     DAOMAX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13078_length_2076_cov_2.261752"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1578)
                     /locus_tag="PK742_13160"
     CDS             complement(100..1578)
                     /locus_tag="PK742_13160"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77587.1"
                     /translation="MKTKLTFILLLGMNLQLIQAQWSIDSVNTPDTYPYAAVNGNKAV
                     FSNGSEWNTFDPISGMHTNGSFTISRQMIEVVSLGDKVLFGGGKYGSFADPLYTKQVN
                     VYNSTSDSWSIWNLGTAREVGAAAGIGNKVVFAGGTGRTDIAGPVKMYNKVDIFDITT
                     GARLSGKISKARTNIAAGAAGNKIVFAGGWYWNGSYSIVFSNKADIYDVTTGLWSSVL
                     LSKKRDNISVAVVGDQIIFAGGSGTAGAVNTVDIYHASTNTWTVSSMPAAGYGYKTAV
                     IGTDAYFTGYAFGDFDAVYRYNTLTGVWNTFYLPTSLSNFSLSTVDGRLVFAGGTVPG
                     TNTYSDLVQIFDPLTDSWSYENLSMARTNVTALNIGNVSYFAGGIQAYAYPSPVPSNR
                     VDIFAAPFRHADPGTEMQHWAIHIYPNPADQFILINLENQDGIPLPLDLKIVDLQGRQ
                     VVRTELYDHQTRIAVDHLAPGVYFILTEDRYGHAENTEMIKQ"
BASE COUNT          529 a          464 c          473 g          610 t
ORIGIN      
        1 ccgggcattg tttcctggcc tgtgaactgc tggcagcaat aggacaggag tatgaactgc
       61 gatatcaaac caccgaagtt catagttgag tggtctgttc tattgtttga tcatttccgt
      121 attttctgca tggccatatc tgtcttctgt cagtatgaag tagacacctg gtgctaaatg
      181 gtcaacagct attctcgttt gatgatcata taattctgtc ctcactacct gacgaccttg
      241 caggtcaaca attttgaggt ctagcggcag cgggatccca tcctggtttt ccagattaat
      301 caggataaat tgatctgcag ggttaggata gatatgtatt gcccaatgct gcatttctgt
      361 tccgggatct gcgtgccgga agggagcagc aaagatgtca acacggtttg aaggaacggg
      421 cgacggataa gcgtaggcct gaattccacc tgcaaagtag ctgacattgc cgatgttcaa
      481 tgcagtcaca tttgtccggg ccatactgag attttcatag ctccatgaat cggtcagagg
      541 atcgaagatc tgtaccagat cgctgtaggt attggtgccc ggaactgtac ctccggcaaa
      601 taccagacga ccgtctacag tacttaaaga aaagttgctt aacgaagtag gcagatagaa
      661 ggtattccag acgccggtaa gggtgttata tctgtatacc gcatcaaaat ctccaaatgc
      721 gtatccggta aaataagcat cagtgcctat caccgcagtt ttataaccat aacctgctgc
      781 aggcatggaa ctgacggtcc aggtattggt agaagcatgg tagatatcta cagtattcac
      841 agcaccggct gtacctgagc ctcctgcaaa aatgatctga tcacctacca ctgctactga
      901 gatattatcc cgctttttag ataataggac actgctccac aaacctgttg ttacgtcgta
      961 aatgtcggcc ttattggaga acacgatgct gtatgatcca ttccagtacc aacctccggc
     1021 aaatactatt ttatttcctg ctgcaccggc tgcaatattc gtgcgggctt tggatatttt
     1081 acccgaaagc ctggctccgg tcgtgatgtc aaatatgtca accttgttat acattttcac
     1141 cggacctgca atatctgttc tgcctgtacc tccggcaaac actaccttat tgcctatccc
     1201 agctgcggca ccaacttcac gtgcggtgcc cagattccag atgctccagg aatctgaagt
     1261 gctgttgtac acatttacct gtttggtata aagtggatct gcaaaggatc catacttgcc
     1321 accgccgaat aataccttgt cgcccaggga gaccacttcg atcatctgcc ttgaaatggt
     1381 aaaggaacca ttggtatgca taccgctaat ggggtcgaat gtattccatt cactgccatt
     1441 tgaaaagact gccttattgc cgttgacagc tgcatatgga taggtatctg gtgtgttgac
     1501 ggagtcaatg gaccattgcg cctgaatgag ttgcagattc attccaagca aaagaatgaa
     1561 agttaatttc gttttcattt gatatatgat ttgttcgttt gataattgtg tggcgtataa
     1621 aactgttgct catggagatc agcatcagga tctcctggtg ttgttggttt gttacaaatg
     1681 attttttctg aattgtttta aggatgattg atatacacat atcctgaccc gatccggccg
     1741 gaagtgttgc gtgctgaatt gttgccatga accagattcc atgaatactc tttctgatat
     1801 ccgggcgatc acagtttatt actacccgtt tatcctgtat tcatgttaag ttatcctgac
     1861 tcactctgtc ggatgaacag gcggatactg ccatccggtg gattgctgct ttttatcttt
     1921 ttttatctcc cgatttactg tgcaaggtag tccacaggat acagacattc agcgtcggcc
     1981 gctgcttgcc gaagtgattt aagtcaacaa atgtgggaga gcgaatgacg agtatactga
     2041 ctgctgatgg aaatatgtat tttcatcacc cgatga
//
LOCUS       DAOMAX010000392         2072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13112_length_2072_cov_5.117501, whole genome shotgun sequence.
ACCESSION   DAOMAX010000392 DAOMAX010000000
VERSION     DAOMAX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13112_length_2072_cov_5.117501"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1496)
                     /locus_tag="PK742_13165"
     CDS             complement(228..1496)
                     /locus_tag="PK742_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77588.1"
                     /translation="MKRRILVLAVLLIPAFAFTQQRQLIPEGITGTNEISNALKWERT
                     NAPNERLTSFLMEFYNMGVWMASDSVLFEYSGSHGHNQFDFIGFQNWYLEPDYSAMNW
                     YNYSGTDWENVQQSFQTFDELDYRTSMQMNYWLSGSWEPSYRYLYSNDATGRIVESTF
                     QFWDGSVFEDDHLIEYEYDGDLPFRETVYDWIGSMWEEYSQTVFNYGPDDRLEYVNFY
                     YWMGIGWETDSRDIYSYNADNQLTSIVHQNYTGTDWEDYWKTELKYDAQGRHNQEIEY
                     TWNGTEWLPYFRQTATLSAGGLPESTLWEYWDGYLWLNYYRFIYRYEEYDDGTVAIEE
                     DPVSWAMKIFPNPATDQMSILVRTGIDGYGNLHITDMYGQLVASLNVPLSTGDNLIHL
                     NLDDLGMDAGQYMVVLRSGSKQFQAKFLVQ"
     gene            1630..>2072
                     /locus_tag="PK742_13170"
     CDS             1630..>2072
                     /locus_tag="PK742_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77589.1"
                     /translation="MKRYILILLLLAVSGIQAQETLTDTIRYSTYNLLNFENEAGNHR
                     SVYFRTIMNELQPDLLTVQELIELPAAQMFLDSVMLPTSPVYNLATFISSYDTDRGLY
                     YNSEQFEFLDAEAIDTELRDINQFHLRHTASGQEFYLFTLHLKAT"
BASE COUNT          521 a          511 c          467 g          573 t
ORIGIN      
        1 ccatggccac ctctttcggg ctcatggatg acgttctgtc gaatgcttcc ttcagttcag
       61 cagcaaatgc tttttcagac aaggctgcaa ggcttaaggt gccggtagca gcagcgatct
      121 gacgaaggaa ctgtcggcga ttgctcatgg taacaggttt tcagtggtaa gttacaacat
      181 acgccaaaac agcaatgaaa aagccccgga atatccgggg ccagtactca ttgtaccagg
      241 aattttgcct ggaattgttt gctgcctgat cgcagtacca ccatatactg tccggcgtcc
      301 atgcccaagt cgtccagatt caggtggatg aggttatcgc cagtgctgag cggcacattc
      361 agcgatgcca ccagttgtcc gtacatgtcg gtgatatgca ggttgccata tccgtcgata
      421 cctgtcctga ccaggatgct catctgatct gttgcgggat tcgggaagat cttcatcgcc
      481 caggaaacag ggtcttcttc aatggccaca gtaccatcat catattcttc atatcgatag
      541 ataaaacgat aatagttcaa ccacagatag ccatcccagt attcccataa cgtactttct
      601 ggtaagccac cggcagacaa agtagcggtc tgtcggaagt acggcagcca ttcggttccg
      661 ttccaggtgt attcgatctc ctggttgtga cgcccttgtg catcatattt taattctgtt
      721 ttccagtagt cttcccaatc tgtgcctgtg tagttctgat gaacgatgga tgtcagctga
      781 ttatctgcat tataggaata gatatctctg ctgtctgttt cccatcctat tcccatccag
      841 taatagaagt ttacatactc cagtctgtca tcgggaccat agttgaatac ggtctgactg
      901 tattcttccc acatagatcc tatccaatca taaacagttt cccggaatgg taaatctccg
      961 tcatattcat attctatcag atggtcatct tcaaatacag atccatccca gaactgaaag
     1021 gtcgattcta ctattctgcc tgttgcatca ttggaataga gatagcgata ggaaggttcc
     1081 cagctgcctg acaaccagta gttcatttgc atgctggttc gatagtccag ttcatcaaaa
     1141 gtttgaaaag attgctgcac attttcccag tctgtaccac tgtaattgta ccagttcatt
     1201 gcggaatagt ctggttccag ataccagttt tggaatccga taaaatcaaa ctgattgtgt
     1261 ccgtgtgaac cagaatactc aaacagcaca gaatcgctgg ccatccagac tcccatgttg
     1321 tagaattcca tcaggaatga ggtaagtcgc tcgttcggtg catttgttcg ctcccatttc
     1381 aatgcgttgc tgatttcatt agtgcctgtg attccctccg ggatcaactg cctctgctgc
     1441 gtgaacgcga acgcaggaat cagcagcacg gcaagcacga gtattcttcg tttcatacca
     1501 catggcgttt gtgcgcaaac tatctgacaa actgctgacg gcaggtgata ggagagacat
     1561 tgttaactga ttgtaattag tttcgggcag gtaacttttg cttgcctgca ttgactaact
     1621 tagcgcaccg tgaaaagata cattctgata cttcttttgc ttgcggtttc gggcatacaa
     1681 gcccaggaaa cgctgacaga taccatacgc tacagcacgt ataacctgct gaactttgaa
     1741 aacgaagcgg gtaaccatcg gtctgtttat ttccgaacca tcatgaacga actgcagccg
     1801 gatctgctta ccgtgcagga gttgatcgaa ctgccggcag ctcagatgtt cctggattct
     1861 gttatgctgc ccaccagccc ggtgtacaac ctggctactt tcatttccag ctacgataca
     1921 gacagaggcc tgtattacaa cagtgagcaa ttcgaatttc tggatgcgga ggcgatcgat
     1981 acggaactgc gtgatatcaa ccagtttcat ttgcgacata ccgcgagcgg acaggagttc
     2041 taccttttca ccctgcacct gaaggcgacc ag
//
LOCUS       DAOMAX010000393         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13150_length_2070_cov_3.201985, whole genome shotgun sequence.
ACCESSION   DAOMAX010000393 DAOMAX010000000
VERSION     DAOMAX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13150_length_2070_cov_3.201985"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PK742_13175"
     CDS             complement(<1..1080)
                     /locus_tag="PK742_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77590.1"
                     /translation="MRKFVLVGFFAIMAAGVSAQEVLRPLSYNPVLRNLNPAENTGGT
                     RDIGTSRDTLCLPFFDDFSNLSRFLDSTSILCGDTVFNSQPSAVYPSNLFWTDSLAFV
                     NNTYPILPPSYGVATLDGLRANGRPHSDLIVNGPADTLTSRPVFLGGSLTDSVYLSFS
                     YQPGGFGDFPELNDSLILEYKMPDNSWQRVWFTRNTEGDVNQPFRTAMVPVADEFQYD
                     GFQFRFRNWANVQGNNDHWNIDYVYLADDRSYTDTLFRDVSFVGPPTSILRHYRQMPW
                     NQFRNHQAEELDDNHLAQIINNFNTVINTSYQYRAFETWTGTEIEPLSAPISINFDPF
                     STAFNSYNTFEIPSSTPGYDGDSLSV"
     gene            complement(1077..1880)
                     /locus_tag="PK742_13180"
     CDS             complement(1077..1880)
                     /locus_tag="PK742_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQU77591.1"
                     /translation="MRFLISKAFWLNLVLAGVFVFLTAWITLGLINRYSRHSESQTVP
                     DLTGLPATEAERVLEQAGLKYAVMDSSYLQEATPLSIISQEPKPGSMVKEGRTIYAVV
                     NRAAPPPTELPYIEQGTSYISVVEILQSRGLRIGNISYKPFQYKDVFLDMRVRGVNGT
                     PKPGTKIPKGSIVDLVLGNGLGETRVEIPDLVGLSYLEASALIQLKELNVGAVVGDGA
                     ITDTMSAFIVRQYPAPYEGGTINMGSMVDIWISDLPPATQDQPENDMHP"
BASE COUNT          528 a          499 c          519 g          524 t
ORIGIN      
        1 tactgacagg ctatcgccat catagcctgg agtggaagat ggaatttcaa aggtattgta
       61 ggaattaaag gccgtactaa aagggtcaaa gttgatagaa ataggagccg aaagtggttc
      121 tatttctgtt cctgtccatg tttcaaaggc acgatactga taactcgtgt tgatgaccgt
      181 gttgaagttg ttgatgatct gtgccagatg gttatcatcc agctcttctg cctggtggtt
      241 tcgaaactga ttccagggca tttgcctgta gtggcgcaga atggaagtgg gcggaccaac
      301 aaaagacaca tcccgaaaca acgtatctgt ataagaacga tcatcggcca ggtaaacgta
      361 atcaatattc caatgatcat tgttaccctg cacgttagcc cagtttctga aacggaactg
      421 aaaaccatca tactgaaatt catccgctac cggaaccatg gcagtgcgga agggctggtt
      481 cacgtctcct tcagtatttc gggtaaacca gacacgttgc cagctgttgt caggcatttt
      541 gtattccagt atcagagagt cattgagttc aggaaaatcg ccaaatcctc cgggctgata
      601 ggagaagctc agatagacgg aatctgtgag tgaaccaccc agaaaaacag gtctggaggt
      661 gagtgtatct gccggaccat tcacgatgag atcgctgtgt ggtctgccat tggcacgcaa
      721 tccgtccagt gtagctacac cataactggg cggcaggata gggtaagtat tgtttacaaa
      781 tgccagactg tctgtccaga acaggttact aggatatacg gcagaaggct ggctgttgaa
      841 cacagtatct ccgcacaata tgctggtgct gtcaagaaag cgactgagat tggagaagtc
      901 atcaaaaaat ggcaggcaga gggtgtctct gctggtaccg atatcgcggg tacctccggt
      961 gttctctgca gggttgaggt ttcgaagtac cgggttatat gaaagcggac gaaggacctc
     1021 ctgagcactg acaccggcag ccatgatggc gaaaaaacct acaagtacaa atttcctcat
     1081 ggatgcatat cattttcggg ctgatcctgt gtggcgggcg gaagatcact gatccagata
     1141 tctaccatgg agcccatatt tatagtacca ccttcataag gagcaggata ctgacgaacg
     1201 ataaacgccg acatcgtatc tgtaattgcc ccatcgccca ctacagcccc gacgtttagt
     1261 tctttcagtt gaatcagggc actggcttcg aggtaactca gaccaaccag atcaggtatt
     1321 tccacccggg tctctcccag cccattaccc agcaccagat ctactattga gcctttggga
     1381 atcttcgttc ccggctttgg cgtgccgttc acaccccgaa cacgcatatc cagaaagaca
     1441 tctttatact ggaacggctt gtagctgatg ttccctatgc gcagtccgcg gctttgaaga
     1501 atctccacta cactgatgta gctggttccc tgttctatgt aaggcagttc tgtgggaggc
     1561 ggtgcagccc tgttcaccac cgcatagatc gttctgcctt ctttgaccat actgccgggt
     1621 tttggctcct ggcttatgat acttaatggt gtggcttcct gaagatagct gctgtccatg
     1681 accgcatatt tcaaaccagc ctgctccagc acccgttcag cttctgtggc cggtaagccc
     1741 gtcagatcag gtactgtctg gctttctgag tgacggctgt atcggttgat taatccaagt
     1801 gtgatccacg ccgtgaggaa cacaaaaact cctgccagca ccagattgag ccagaaagct
     1861 ttactgataa gaaagcgcat gaagcctggg cgggtttctt tatttgtctt tttgggcaac
     1921 agccgcagtt ttaatgagcg gtaaatttac aaagtctcat ccagcaacct ggtgaagaat
     1981 tccgtcaggc taagtccgag gccctggcca tctgcggcac tatgctggct gcgctcaaac
     2041 ccggaacggt attaacctct aaaaagtgtg
//
LOCUS       DAOMAX010000394         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13203_length_2066_cov_3.677772, whole genome shotgun sequence.
ACCESSION   DAOMAX010000394 DAOMAX010000000
VERSION     DAOMAX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13203_length_2066_cov_3.677772"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..308
                     /gene="rpmF"
                     /locus_tag="PK742_13185"
     CDS             123..308
                     /gene="rpmF"
                     /locus_tag="PK742_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQU77592.1"
                     /translation="MPHPKQRHSKQRTRTRRSHHALEQPHVMNCSNCGAPVLRHRVCT
                     ECGFYRGKVAIDKSSEN"
     gene            335..1333
                     /locus_tag="PK742_13190"
     CDS             335..1333
                     /locus_tag="PK742_13190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQU77593.1"
                     /translation="MSRIYAAITGVNGWVPEYRLTNAELEKMVDTNDEWITTRTGIKE
                     RRILKGEGLATSDMAAEAVKGLLKKTGTDASEIDMLLVATTTPDMPFPATANIVCDKI
                     GAGHILSYDIGAACSGFLYALTTASKFIETGTYKKVVVVGADKMSSIINYNDRATCVI
                     FGDGAGAVMLEPSTEKVGVIDSVLKSDGSGRHFLHQKAGGSLKPATLETVANQEHSVY
                     QEGKTVFKFAVKNMADVAEEIMVRNELQGDDIAWLVPHQANKRIIDATAERMHIPEEK
                     VMINIERYGNTTAGTLPLCLWEWEDKLHKGDNLVLAAFGGGFTWGATLIKWAYDPQ"
     gene            1345..1908
                     /gene="efp"
                     /locus_tag="PK742_13195"
     CDS             1345..1908
                     /gene="efp"
                     /locus_tag="PK742_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQU77594.1"
                     /translation="MASTADIRNGLCIDFNHDIWQFVEFLHVKPGKGNAFVRSKLKSL
                     TSGKVVEHTFPSGATLNEVRVERRVYQFLYPDEFGFNFMNNETFEQVSIPEDMIDGKQ
                     FLKEGQEVEILFHADTERPLLCELPTHIVLEVIEAEPGVKGNTATNATKNATLETGAT
                     IQVPLFVDAGEKIKIDTRTKSYVERVK"
BASE COUNT          624 a          459 c          504 g          479 t
ORIGIN      
        1 cccccggttg taatccggat atcctggccc ggttaggaca taacgaagat cagccgaaag
       61 attcagaccc cagatgggat caactaagaa cgatcataaa agacaagtaa aaacagagcg
      121 tcatgccaca tcctaaacaa cgtcactcca agcaacgcac ccgtacacgt cgttcacatc
      181 atgccctgga acagccgcat gtaatgaact gttccaactg tggtgccccg gtacttcgtc
      241 atcgtgtgtg caccgaatgc ggcttctacc gcggcaaggt tgccatagat aaaagcagcg
      301 aaaactgatc agtcaccgaa tctgagttga actgatgtcc aggatctacg cagctatcac
      361 cggtgttaac ggttgggtac ctgaataccg tttaaccaac gcagagttgg aaaagatggt
      421 ggataccaat gatgagtgga tcaccacccg cactggtatc aaagagcgcc gtatcctgaa
      481 aggggaaggt ttggccacat cagacatggc cgctgaagcg gtgaaaggtt tgttgaaaaa
      541 aaccggtacc gatgcttcag agatcgatat gctgttggtt gccaccacta ctcctgacat
      601 gccatttccg gctactgcca acatagtttg cgacaagatc ggagcgggtc acatactcag
      661 ttatgatatt ggtgctgcct gcagtggttt tctatatgca ttgaccactg cttccaaatt
      721 catagagacc ggcacttaca aaaaagtggt ggtagtgggt gcagacaaaa tgagcagcat
      781 catcaactac aatgaccgtg caacctgtgt catattcgga gatggcgcag gggcggtcat
      841 gctggaaccc agcacggaaa aagttggtgt gattgattcg gtgctgaaat cagacggttc
      901 cggaagacat ttcttacatc aaaaagccgg tggatctctt aaaccagcta ctttagagac
      961 cgttgccaac caggagcaca gcgtgtatca ggaaggtaag acagttttca aatttgccgt
     1021 caaaaacatg gcagatgtag ctgaagagat catggtacgg aatgagcttc agggcgatga
     1081 tattgcctgg ctggtgcccc atcaggccaa caaacgcatc attgacgcta cagcagagcg
     1141 catgcatata ccagaagaga aagtgatgat caatattgaa aggtatggca ataccactgc
     1201 aggcaccctt cctctgtgtc tttgggaatg ggaagataaa ctccataaag gtgacaatct
     1261 tgtgctggca gcatttggag gtggtttcac ctggggtgcc acccttatca aatgggcata
     1321 cgacccccaa taaccaaata acttatggca agtacagcag atatcagaaa cggcctgtgt
     1381 atcgatttta accatgacat ctggcagttt gtagagtttt tacatgtaaa gccgggtaaa
     1441 ggcaatgctt ttgttcgcag caagctcaaa agtctgacca gtggtaaggt ggttgaacat
     1501 acatttccat caggcgctac tttgaatgag gtcagggtgg agagaagggt gtatcagttc
     1561 ctgtatccgg atgaattcgg ttttaatttc atgaataatg aaactttcga gcaggtgagc
     1621 ataccagaag acatgataga tggtaaacag ttcctgaaag aaggacagga agtggagatc
     1681 ctttttcatg cagatacaga aagacctcta ttgtgcgaac ttccaaccca tatcgtgctg
     1741 gaagtgatcg aagctgaacc cggtgttaag ggaaatactg ctacaaatgc cacaaagaat
     1801 gctaccttag aaaccggagc aaccattcag gtacctctgt ttgtagatgc cggtgaaaaa
     1861 atcaagattg acacccgcac caaaagctac gttgaacgag taaaataatt ttcatggact
     1921 tcaaacaaat tcaggaactt atcaaactgg ttggcaaaag cggcatatct gagctgtcaa
     1981 tcgagaacca ggatatcaaa ctgaagatca agacaggaac tacttcacat gataccccgg
     2041 tagttatgcc tcaaatggtc atgcct
//
LOCUS       DAOMAX010000395         2062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13253_length_2062_cov_3.852516, whole genome shotgun sequence.
ACCESSION   DAOMAX010000395 DAOMAX010000000
VERSION     DAOMAX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13253_length_2062_cov_3.852516"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK742_13200"
     CDS             complement(<1..563)
                     /locus_tag="PK742_13200"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77595.1"
                     /translation="MKWISGILMTVTTGVFAGNNDAVQAVIDLTSVQNDKVQVEVDLP
                     DMQESTVVYCLPKIVPGTYSIYDFGRFVSDFKAIDKSGAEMAVEQKSVNEWKITNATQ
                     LDKISYWVEDTYDATYDNPIFEPAGTNIEEGKNYLLNTFGFIGYVQGQKENDIRLTVR
                     KRPGDYGVSPLQAIQSTSDHDIYHLDN"
     gene            869..1435
                     /locus_tag="PK742_13205"
     CDS             869..1435
                     /locus_tag="PK742_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQU77596.1"
                     /translation="MPSYLDSIRKQFHYYQQLGEKTFEQVADDKLFWQYNEESNSMAT
                     IVKHLWGNMLSRWTDFLTSDGEKEWRQREAEFDNDIRSRDDMMAKWTEGWNCLYAAID
                     PLTDEDLEKIIFIRNQGHTVTEAINRQLAHYAYHVGQIVLLGKMLADQWDSLSIPRGR
                     SDAFNKEKFSRPKEKGHFTDEFLDQPEK"
     gene            1482..>2062
                     /locus_tag="PK742_13210"
     CDS             1482..>2062
                     /locus_tag="PK742_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77597.1"
                     /translation="MKPVSALAALLLPLVTIAQQEPKWDVNTINIPYTDVTISTSEGT
                     WMSLDVSPDGKEIVFDMLGDIYTLPIAGGEATLLRGGHAFEVQPRYSPDGKWISFTSD
                     AGGGDNIWIMHRDGSDAKQITKEDFRLLNNAVWSADGQYLFARKHFTSQRSLGAGEIW
                     MYHMSGGQGIQLTKRKNDQQDVGEPWASPDGRYV"
BASE COUNT          526 a          522 c          490 g          524 t
ORIGIN      
        1 taattatcca ggtggtagat gtcatggtca gaggttgatt gaatggcctg cagcggactt
       61 acaccataat ctcccggacg tttgcgcaca gtcagccgga tgtcattctc cttctgccct
      121 tgcacatagc ctataaatcc aaatgtattc agcagataat tctttccttc ttcaatgttg
      181 gtacctgccg gttcgaagat gggattgtca taggtagcat cataggtgtc ttccacccag
      241 taggatatct tgtccagctg agtggcattg gtgatcttcc attcattcac gctcttctgt
      301 tctacagcca tttcagcacc tgacttatca atggctttga aatcgctcac aaatcttccg
      361 aagtcatata tggaataagt gcccggcacg atcttcggca ggcaatacac cactgtactc
      421 tcctgcatat caggcagatc tacttccacc tgtaccttat cattttgtac actggtcagg
      481 tctatcactg cctgcacagc atcattgttt ccggcaaata caccggtagt tacggtcatc
      541 agaataccac tgatccattt cattttgttg ggttttgccc caaaactatc gggcatgcat
      601 cttaagaacc gttaaagcaa aatgtattta acaggcgttc agatttttta acagaggggg
      661 aggcgtggtt cgttgtgcgt ggttcgtttt gcgttgttcg ttatcgtttt gcgtggttcg
      721 tttttcgtcg atcgttgtgc gtttcggata ttggttatct ttcaggtctg aacatttatg
      781 gccaactcct cactcaccac tccaactccc actccaactc ccagtcccac tcccactctc
      841 actcccactc aacaaaccca ctcccaccat gccctcctac ctcgacagca tccgcaagca
      901 attccattac taccagcagc tgggtgaaaa gacctttgaa caggtggctg acgacaagct
      961 gttctggcag tacaatgaag aaagcaacag catggccacc atcgtgaaac acctgtgggg
     1021 aaacatgctc agcaggtgga ccgattttct gaccagcgac ggagagaagg aatggaggca
     1081 gcgggaagca gagttcgaca atgacatccg ttcccgtgat gacatgatgg cgaaatggac
     1141 ggaaggatgg aattgtttgt atgcagccat cgatccgctg accgacgaag acctcgaaaa
     1201 gatcatcttc attcgcaacc agggacatac cgttaccgaa gcgatcaatc gtcagcttgc
     1261 tcactacgcc tatcacgtgg ggcagatcgt gttactgggg aagatgctgg cagatcagtg
     1321 ggacagcctg agtattccaa gaggaagatc ggatgcattc aacaaggaaa aattttcccg
     1381 tccgaaagag aaagggcatt tcaccgacga atttctggat cagccggaaa agtgaacacc
     1441 atccggtgat tgattagatt taccgattcc aacagatgta tatgaaacct gtatctgcac
     1501 ttgctgcgct gcttctgccg ttggtcacca ttgcccaaca ggaacccaaa tgggatgtga
     1561 acaccatcaa cattccttat accgatgtga ccatctctac ctctgaaggc acctggatga
     1621 gtctggacgt cagtcccgat ggcaaagaga tcgtgttcga tatgctgggt gatatctaca
     1681 ccctgcccat tgccggcgga gaagcgacgc tgcttcgagg cggacatgcc tttgaagtgc
     1741 aaccgcggta tagtcctgac ggaaagtgga tctccttcac cagcgatgcg ggtggtggcg
     1801 acaatatctg gatcatgcac agagatggat cagatgcaaa acagatcacc aaagaagatt
     1861 tccgtctgct gaacaatgcg gtctggtcgg ccgatggtca gtaccttttc gcacgcaaac
     1921 atttcacttc tcaaagatcg cttggtgccg gtgagatatg gatgtatcac atgtcgggcg
     1981 gacaaggcat ccagctgacc aaaagaaaga atgaccagca ggatgtagga gaaccctggg
     2041 cttcccctga tggacgatat gt
//
LOCUS       DAOMAX010000396         2055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13341_length_2055_cov_3.573500, whole genome shotgun sequence.
ACCESSION   DAOMAX010000396 DAOMAX010000000
VERSION     DAOMAX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13341_length_2055_cov_3.573500"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PK742_13215"
     CDS             <1..403
                     /locus_tag="PK742_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77598.1"
                     /translation="FGLINQLYDMQTKNLFLTGAALLLTLPLQFCTASKSATKTTEET
                     TTTEPTVSYSRDLAPIMEAKCTPCHFPETGKKKLLNTYAAVSESSDYILFRIQLPEDD
                     PEFMPFKGKKPALTPEEVQLFVQWAAEGKAE"
     gene            complement(468..1625)
                     /locus_tag="PK742_13220"
     CDS             complement(468..1625)
                     /locus_tag="PK742_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQU77599.1"
                     /translation="MRVRYCLAAFILLSELASYGQLPQDNFEIRSDVWGTTRQVKVWM
                     PESYLEDDTTSFAVLWVLDAQSETYWQMVCGNLAYLQSKDQIIPCIAVGIVSPDRSYE
                     YSPLHRELADHLQIELMPYIRDHYRTTPLDIVMGHSWAGAFVANTLFSDDSDLFEAYL
                     AISPSLSAIDGRILNQADSVLVAEMKTPKVFYCATGDLGIEEAESIEDMAAMSEICLK
                     YSNPALAWLPSRIEKTDHFSCVAPAMVDGLLSICSRYMASERMVNNWVVETGGEILPL
                     LEIYGKYNGHSFGYWYSPPVSYWGKLADSYLQLEEFITARDLYLFGLQNGDENVVNAF
                     NLALTYEALEDRGNARKHFLRADELLEQQKDQIKESLYRSLKKAVGEKLGE"
     gene            complement(1652..>2055)
                     /locus_tag="PK742_13225"
     CDS             complement(1652..>2055)
                     /locus_tag="PK742_13225"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77600.1"
                     /translation="PGETSLAYLASTPGNYQVEVTNAEGCSKLSNKRKVISSGCRMAA
                     EGEEMTLAVYPNPTRNLLNYQLFYDDNMQETVTLQIHDLQGRILLTDQEMLHNGICSG
                     AIALDLPSGFYVLKAQTRDGRYLLSPFVIER"
BASE COUNT          543 a          540 c          441 g          531 t
ORIGIN      
        1 tttcggactc atcaaccaac tgtatgacat gcaaacaaag aacctctttc tcactggagc
       61 agccctgctg ctgacgcttc cgttgcaatt ctgtacagca tctaaatcag ccaccaagac
      121 caccgaagag actacaacaa cagagccaac tgtttcctat tcccgcgacc tggcacctat
      181 catggaagcc aagtgcacgc cctgccactt cccggaaaca ggcaagaaga aactgctgaa
      241 cacctatgct gctgtgagcg aaagctccga ttatatcctc ttccgcatcc agctgcctga
      301 agatgatccg gaattcatgc cttttaaagg aaagaaaccg gcactgacgc ccgaagaggt
      361 tcagcttttc gtacagtggg cagcagaggg gaaggcggaa taatagaaat gcggttttag
      421 aaggacgaaa ggcggataag agttggtctc tcccttcgat ctgatcgcta ctcacccaac
      481 ttctctccaa cagctttttt cagcgaccgg tacagacttt cttttatttg atctttttgc
      541 tgctccagta gttcatcggc acgaaggaag tgctttctgg cattcccccg atcttccagt
      601 gcctcataag tcagcgccag gttgaacgca ttgaccacat tttcatcgcc gttctgcaaa
      661 ccaaaaagat agagatcgcg ggcagtgata aattcttcta gttgcaggta actgtctgcc
      721 agtttccccc aataagaaac cggtggtgaa taccaatagc caaaagaatg gccattatat
      781 tttccatata tctcgagcag gggcaagatc tcaccacctg tttcaaccac ccagttgttc
      841 accattcttt ctgacgccat atacctgctg caaatggata acagtccgtc caccatggca
      901 ggtgcaacac aggaaaaatg gtctgtcttt tctatccttg aagggagcca tgccaatgca
      961 ggattggaat acttcaggca tatctcagac atcgcggcca tatcctctat ggattcagct
     1021 tcttctatac ccagatcgcc agttgcacaa tagaatactt ttggcgtttt catttcagcc
     1081 accagcacag aatcagcttg attcaaaata cggccatcaa tggcactcaa agaagggctg
     1141 attgcaaggt aggcctcaaa cagatcgcta tcatcgctga ataaggtatt ggccacgaat
     1201 gctccggccc agctgtgtcc catgacgata tcgagcggag tggtcctgta gtgatcacgt
     1261 atgtacggca tcagttctat ctgcagatga tcagctaatt cacggtgcag aggtgaatac
     1321 tcataacttc tgtcgggcga tacaatgccc actgcaatgc atgggatgat ctggtctttg
     1381 gactggaggt aggccaggtt gccacacacc atttgccagt aagtttctga ttgtgcatcc
     1441 aatacccaca acactgcaaa gctggtggta tcatcctcca gataagattc cggcatccac
     1501 acctttacct gtcttgttgt tccccagaca tctgacctga tctcgaaatt atcttgtgga
     1561 agctggccat aagaagctaa ctccgacaac aggataaatg ctgccagaca gtatcttacc
     1621 cgcataccgc atgctttcat aacacctgta ctcaacgctc aattacaaat ggtgataaca
     1681 aatatctgcc atctctggtc tgtgccttca gcacatagaa tccggatgga agatccaaag
     1741 caatagcccc gctgcatata ccattatgca acatttcctg atcggtcaaa aggatccgtc
     1801 cctgcaagtc gtgtatttgc aatgttactg tttcctgcat gttgtcatca taaaacaact
     1861 gataattcaa caggttgcgg gttgggttag gatagacggc aagtgtcatc tcctctcctt
     1921 ctgcagccat cctgcaacca ctgctgatga cctttctttt gttggagagc ttggagcacc
     1981 cctcggcatt ggttacttct acctggtaat tgcctggagt tgatgccagg taagccagag
     2041 atgtttctcc cggta
//
LOCUS       DAOMAX010000397         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13402_length_2050_cov_3.892732, whole genome shotgun sequence.
ACCESSION   DAOMAX010000397 DAOMAX010000000
VERSION     DAOMAX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13402_length_2050_cov_3.892732"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK742_13230"
     CDS             <1..357
                     /locus_tag="PK742_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77601.1"
                     /translation="TPGLDTMELLMKLSVKLVFCDTARVNRFTIDLPDNYMVASLSYA
                     GFGDLRTRYDLHAIATSANQATRITVFHGPCDIIPLEAAAAIRDDHQVVSLNKCGHYP
                     FIERPGYFLRMVEKAL"
     gene            406..1050
                     /locus_tag="PK742_13235"
     CDS             406..1050
                     /locus_tag="PK742_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU77602.1"
                     /translation="MSVSVIVAHQHALVREGLSHILQEMPDAELHATCTNAADLLPML
                     ESGSGVLILDPLHVDGMELQDVELLQQFSGWKVLLITACEQMAMVRQLVGTGIQGYLT
                     RTCDRQEIEEAIMRVAKGETTYCHKVLDILMGQAPADSERCDPAILSEREVEIIRLIA
                     SGLTTREIAGKLYRSEHTISTHRKNIMRKLNLRSSSELMVYAMASGLILQEGRA"
     gene            1191..>2050
                     /locus_tag="PK742_13240"
     CDS             1191..>2050
                     /locus_tag="PK742_13240"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQU77603.1"
                     /translation="MKIPRIRVLIMLAAIAIIGIVITQVFWIRKGLLINQTSFENAVV
                     VTLDRIANNIWLKPDIPVGDRPQVIRISPRSYLVQMNMPIDLNYLDNQLRGEFTNPFH
                     KIDFTYEVFEHSTDMMVFSDSVYVELADDDFHEARYLPNLTESGYYFKVNFPQRPIVP
                     SIMITIWATAILILTTVIIFFTYSLVVIVRQQRLSDFQNAFINNLAHEFKTPLSTIGI
                     SAGVLKEPEIIHDPKRLQVYSNIIELENERLKGQVNKILELARLENREIILRKEPINL
                     RQLLEEIVPP"
BASE COUNT          525 a          510 c          503 g          512 t
ORIGIN      
        1 actcccggat tggataccat ggaactgttg atgaaacttt cggtaaaact ggttttctgt
       61 gataccgcca gggtcaaccg attcaccatc gaccttcccg ataattatat ggtcgcctcg
      121 ctgagctatg ccggattcgg cgatctgcgc acccgctatg atctgcatgc catcgccaca
      181 tcggccaacc aggccacccg gatcactgtt tttcatggcc cctgcgacat catcccgctt
      241 gaggcagccg cagcgatcag agatgatcat caggtggtta gccttaataa atgtggtcat
      301 tatccattca ttgaacggcc gggttatttc ctgcggatgg tggaaaaagc attgtaatct
      361 tcccggtcct ttcctatctt cgtgtttaga atcattctaa acaaagtgtc ggtttcggta
      421 atagtggccc atcagcatgc actggtccgt gaaggactct ctcatattct gcaggagatg
      481 cctgatgccg aactgcatgc cacctgcacc aatgcagccg atctgcttcc gatgctggaa
      541 tccggcagcg gggtcctgat ccttgatcca ctgcatgtgg atgggatgga actgcaggac
      601 gtggaattac tgcagcagtt ttctggttgg aaagtattgc tcattactgc ctgtgaacag
      661 atggcgatgg tcaggcaact tgttggcaca ggcattcaag gatacctgac ccggacctgc
      721 gacagacaag agatcgaaga ggccattatg cgtgtggcca aaggagagac tacctattgt
      781 cataaggtgc tggatatcct gatgggacag gcacctgcgg atagtgaacg ttgtgatccg
      841 gctatactat ctgaaagaga agtagagatc atacggctta tagccagcgg actaaccaca
      901 cgagagatcg ccgggaaatt ataccgaagc gaacatacca tctccaccca ccgcaagaac
      961 atcatgcgga agctgaacct cagaagcagc tctgaactca tggtatatgc gatggccagc
     1021 gggctcatct tgcaggaagg acgagcgtag taggatgagt gggaggggtg agtgagagtg
     1081 agagtgaccc tgtaccctct acttgatacc tgctaattct ttcccactac tcaataccca
     1141 ctacccaata ctcaatactc aatactcaat acctttgtag tctgtagcag atgaagatcc
     1201 cccggatacg agtactgatc atgctggcgg ccattgccat cattggcatt gtcatcaccc
     1261 aggttttctg gatccggaaa ggtctgctca tcaaccagac cagctttgag aatgcggtgg
     1321 tggttactct tgatcggatt gccaataaca tctggttaaa gcctgacatt cctgttggcg
     1381 acagaccgca ggtgatacgg atttctccga ggtcatacct ggtgcagatg aacatgccta
     1441 ttgaccttaa ttatctggac aaccagctgc gtggcgaatt taccaacccg tttcacaaga
     1501 tcgactttac gtacgaagta tttgagcaca gcacagatat gatggtgttc agtgattcgg
     1561 tatatgtaga actggctgat gatgactttc atgaagcaag gtacctgcca aacctgaccg
     1621 aatcaggata ctatttcaag gtcaattttc cgcagcggcc tattgtaccc agtatcatga
     1681 tcaccatctg ggcaactgct attctgatcc tgactacggt catcatattc tttacctact
     1741 ccttggtggt cattgtgaga cagcagcgcc tcagtgattt ccagaatgcc ttcatcaaca
     1801 acctggcgca tgaatttaag acgcctttgt caaccattgg catctctgcc ggtgtgctga
     1861 aagagccaga gatcatccat gatcccaaaa ggctgcaggt ttacagcaat atcattgaac
     1921 tggagaacga gcggctgaaa ggacaggtca acaagatcct ggaactggca agactggaga
     1981 atcgtgagat cattctgagg aaggaaccca tcaaccttcg tcagctgctg gaagagatcg
     2041 ttcctccctt
//
LOCUS       DAOMAX010000398         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13423_length_2048_cov_4.056698, whole genome shotgun sequence.
ACCESSION   DAOMAX010000398 DAOMAX010000000
VERSION     DAOMAX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13423_length_2048_cov_4.056698"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /gene="leuS"
                     /locus_tag="PK742_13245"
     CDS             <1..1983
                     /gene="leuS"
                     /locus_tag="PK742_13245"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQU77604.1"
                     /translation="LDTLDWSESMKDMQRNWIGRSEGASVHFQLKDHDAQIEVFTTRP
                     DTIFGANFMVLAPEHELVAKITTPDQKKEVDEYLAYVKTRSERDRQAEVKKVTGQFTG
                     AYAINPFSEKPIPVYIAEYVLAGYGTGAIMAVPSNDERDNAFAQKFGLDIIPVVDQSA
                     HPNAGMEEKVGVMQNSGFLDGMEVKEAIAKIIGKIESKGIGKRKINYRLRDAGFSRQR
                     YWGEPFPVVYDNDMPELLDESELPLLLPDVEEYKPGGQARSPLAALTDWVNLPDGKVR
                     ETDTMPGYAGSSWYFLRYMSPKHEGRFVAEEAEQYWRSVDLYIGGTEHAVGHLLYSRM
                     WQKFLFDRGWVSEDEPFRKLVNQGMIQGRSSIVYRVNGTNQFVSGGLKDQYETTPIHV
                     DVKLVDGDELDTEGLKQWRQDFADATFILEDGKYFCGHEVEKMSKRYHNVTNPDDVIA
                     RHGADCFRMYEMFLGPIDASKPWDTKGITGVSGFLRKLWRLYVGDNNEWKVSDSEPSA
                     DSLKTLHKTILKVGEDVERLSFNTAVSAFMIAVNQLSEEKCNSRKVLEPLLILLAPFA
                     PFITEELWSRMGYAGSVHEQNWPEADESLLVENSFNYPVSINGKHRTNVEFPLDMPKG
                     DMEQAVLALEAVVKWTEGKLPKKVIIVPGRIINIVV"
BASE COUNT          561 a          476 c          591 g          420 t
ORIGIN      
        1 ctcgataccc tggactggag cgagagcatg aaagacatgc agcgcaactg gatcggcagg
       61 agtgaaggag ccagtgtgca ctttcagctg aaagatcatg acgcacagat agaagtattc
      121 accacccgac ctgataccat cttcggtgcc aacttcatgg tactggcacc ggaacacgaa
      181 ctggtggcga agatcaccac accggatcag aagaaagagg tggatgagta cctcgcttat
      241 gtgaagacca gaagtgaacg cgacagacaa gccgaagtga agaaggtgac cggtcagttc
      301 acaggagctt atgccatcaa tcccttctca gagaaaccca ttccggttta catagcagaa
      361 tatgtgctgg caggttatgg taccggtgcc atcatggcgg tgcccagcaa cgatgagcgg
      421 gataacgcct ttgcacagaa attcggcctg gacatcattc cggtggtgga ccagagtgca
      481 cacccgaatg ccggtatgga agagaaagtt ggggtgatgc agaacagcgg tttcctggat
      541 gggatggaag tgaaagaagc cattgcaaag atcatcggaa agatagaaag caaaggcatc
      601 ggcaaacgaa agatcaacta tcgtctgcgt gatgcaggat tctcccgtca gcgctactgg
      661 ggcgaaccat ttcccgtggt atatgacaac gacatgcccg aactgctgga tgaaagcgag
      721 ctgcctttac tgctgccgga tgtggaagaa tataagccgg gcggacaggc aaggtcaccg
      781 ttggcagccc tgaccgactg ggtcaacctg cccgatggta aggtacggga gaccgatacc
      841 atgcccggat atgccggttc cagctggtac tttctgcggt atatgtcgcc gaagcacgaa
      901 ggtcgtttcg ttgccgaaga agcagaacaa tactggcgca gtgtggacct ctacatcggc
      961 ggaacagaac atgctgtagg tcatctgctg tacagccgca tgtggcagaa attcctgttc
     1021 gaccgcggat gggtgagtga agacgaacct ttcagaaaac tggtcaatca ggggatgata
     1081 cagggacggt cgtcgatcgt gtatcgtgtc aacgggacga atcaatttgt ttctggcgga
     1141 ctgaaagatc agtatgaaac aacacccatt cacgtggatg tgaaactggt ggatggtgat
     1201 gaactggata cagaaggatt gaagcagtgg cgacaggatt tcgctgatgc caccttcatt
     1261 ctggaagatg gaaagtactt ctgcggacat gaagtagaaa aaatgtccaa gcgatatcac
     1321 aatgtcacca accccgacga tgtcatcgcc cgtcacggag cagattgttt ccgcatgtat
     1381 gagatgttcc tcggtcccat tgatgcctcc aagccctggg ataccaaagg catcaccggt
     1441 gtgagtggtt ttctgcgcaa gctctggcgt ttgtatgtcg gagataacaa cgagtggaag
     1501 gtgagcgaca gtgaaccatc ggccgacagt ctgaagaccc tgcacaagac catcctcaaa
     1561 gtgggagagg atgtggagcg tctcagcttc aacaccgccg tcagcgcctt catgatcgcc
     1621 gtcaaccaat tgagtgagga gaaatgcaac agccgtaaag tgctggaacc attgctgatc
     1681 ctgctggcac cgtttgcgcc gttcatcacg gaagaactgt ggagccggat gggatatgcc
     1741 ggaagtgtac atgagcaaaa ctggcccgag gcagatgaaa gtctgctggt agagaacagc
     1801 ttcaactatc cggtcagcat caacggcaaa caccggacga atgtggagtt tccgctcgat
     1861 atgccgaaag gtgatatgga acaggccgta ctggcactgg aagctgttgt caagtggacc
     1921 gaaggcaagc tgccgaagaa ggttatcatc gtcccgggca ggatcattaa tattgtagta
     1981 taacagaacg ggaattgatt cctgtttgtt gacagcatat gcagcaggtc aagatcatcg
     2041 aatgtccg
//
LOCUS       DAOMAX010000399         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13454_length_2046_cov_3.118031, whole genome shotgun sequence.
ACCESSION   DAOMAX010000399 DAOMAX010000000
VERSION     DAOMAX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13454_length_2046_cov_3.118031"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK742_13250"
     CDS             complement(<1..285)
                     /locus_tag="PK742_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77605.1"
                     /translation="MENNTHHNDLSGKLYHHTVSPSDAVWNRIQAQLDADLAAFDTGN
                     KQRFWWYGGSLLAVAAIVFLTWNHYNTLHMAAYEPVATGSQMAIQKSTYST"
     gene            complement(275..853)
                     /locus_tag="PK742_13255"
     CDS             complement(275..853)
                     /locus_tag="PK742_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQU77606.1"
                     /translation="MISEKISPNTPDEELIVQCLENNRRAQKALYDKYASKMMAVCIR
                     YSNDVPAAEDILQEGFVKAYRNLDKFRFDGSFEGWLRRIIVNTAIEHYRKKNHLYPLL
                     ESAEGELEISNDNVLQQLAAEDIMKLIQELSPGYRTVFNMYVIEGYSHKEIAKQLNIS
                     EGTSKSQLARARYLLMDEINKHLGHYRKASGE"
     gene            complement(889..1479)
                     /gene="rdgB"
                     /locus_tag="PK742_13260"
     CDS             complement(889..1479)
                     /gene="rdgB"
                     /locus_tag="PK742_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQU77607.1"
                     /translation="MKWVFATGNKHKVQEIRPLLPVGMELLSLRDIGCDEDLPETGST
                     IAANSLEKAVYVREKYGYPCFAEDSGLVVPAIDGEPGVYSARYAGTGDAGDNIIKLLA
                     RMRGVDDRRAYFVTVFTLAGDGSLEQFEGRADGEITLIPAGEGGFGYDPVFRPKGHDR
                     TFAEMLPDEKREISHRSKAFAQLLIRLKSFENSNKL"
     gene            complement(1476..1931)
                     /locus_tag="PK742_13265"
     CDS             complement(1476..1931)
                     /locus_tag="PK742_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77608.1"
                     /translation="MLSFQKALLNEQIFLIRGTTKVANPPESNSDISYSGSPDSPLFL
                     LYQPEKKELPKPVQEMLAKLLKAIPGLKNEVLTINLSTVTEDALDSFLKAHKPSMIVC
                     HHPRFSAFRPGFSWPAGSRILPAPSLEQLFRDQQAKRSYWAELQNFLAV"
BASE COUNT          487 a          485 c          489 g          585 t
ORIGIN      
        1 agtactatat gtacttttct gtatggccat ctgagaaccg gtagcaactg gttcataggc
       61 ggccatgtgc aaagtgttgt aatgattcca ggtgaggaat acaatggctg caacagccag
      121 cagcgagccg ccataccacc agaaacgctg tttgttgccg gtgtcaaatg cggccaggtc
      181 agcatccagt tgtgcctgga tcctgttcca cacagcgtca gatggggaga cggtgtggtg
      241 atacagcttg ccgctgagat cgttatggtg tgtgttattc tccacttgcc ttacggtaat
      301 gccctaaatg tttgttgatc tcgtccatga gtaagtacct ggcacgggcc aactgactct
      361 tagacgttcc ttcactgatg tttagttgct tcgctatttc tttatgtgag taaccttcta
      421 tgacatacat attaaaaacc gtgcgatatc cgggtgataa ttcctggatc agtttcataa
      481 tgtcttcagc agcaagctgt tgtaaaacat tgtcgttgct gatctccagt tcaccttcag
      541 ctgattccag caggggatat aagtggtttt tttttctgta gtgctcgatg gctgtgttta
      601 caatgatgcg tctgagccat ccttcgaagg aaccgtcaaa ccggaattta tccagattgc
      661 ggtaggcttt cacgaatcct tcctgtaaaa tgtcttcagc ggcaggtaca tcgttggagt
      721 aacgaataca aactgccatc atcttggatg catacttatc atacagcgct ttctgcgccc
      781 gcctgttatt ttccaggcat tgtacgatga gttcttcgtc tggtgtgttc ggggatatct
      841 tttcagatat catgttcaac tagggtttag cgatgctgca cgttaaaatt acaatttatt
      901 tgaattttca aaggatttca atctgatcag cagctgtgca aaagctttgc ttcggtgact
      961 aatctctcgt ttttcatcag gtaacatttc ggcaaacgtg cggtcatggc ccttgggtct
     1021 gaataccgga tcgtacccga atccaccttc accggcaggg atcagggtta tttcaccatc
     1081 ggcccgtcct tcaaattgtt ccagagaacc atcgcctgca agtgtaaaga ctgtcacaaa
     1141 atatgccctt ctgtcgtcca caccacgcat tcttgccaat aatttgatga tgttatcacc
     1201 ggcatctccg gttcctgcat agcgggcgct gtacactccc ggctcaccgt caatggcagg
     1261 caccaccagt ccactgtctt ctgcaaagca cggatagccg tacttttcac gcacataaac
     1321 ggccttctcc aaagaattgg cagcaatggt ggaaccggtt tcgggaaggt cttcatcgca
     1381 gccgatgtct ctcaggctca gcaattccat accaactgga agcaagggtc tgatctcctg
     1441 cactttgtgt ttgttaccgg tggcaaagac ccatttcata ctgccagaaa gttttgcagt
     1501 tcagcccagt atgacctttt ggcctgctgg tcgcggaata gttgttccag actgggtgcc
     1561 ggtaaaatgc ggcttccggc cggccaggaa aatccgggac gaaaagcaga aaaacgcggg
     1621 tgatggcaga cgatcataga cggtttatgt gccttcaaaa aactgtccag cgcatcttcc
     1681 gttactgtgg ataaatttat ggtgagcact tcatttttca gacccggaat agccttcaga
     1741 agcttggcca gcatttcctg cacgggtttg ggcagctcct ttttttccgg ttgatagagc
     1801 aggaaaagtg gcgaatcagg actgcctgag tagctgatgt cactgttact ctcgggcgga
     1861 ttggctacct ttgtggtgcc ccggatcagg aatatctgtt cgttaagaag ggctttctga
     1921 aaacttaaca taccggcttt gtccgttgtg ttgacaggta aataaaacga ttatttcaca
     1981 atatcaaact ggagcatatg gcaataacgg aagcgggcac ctatcatata gatattcgtc
     2041 cggtac
//
LOCUS       DAOMAX010000400         2025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13699_length_2025_cov_4.272589, whole genome shotgun sequence.
ACCESSION   DAOMAX010000400 DAOMAX010000000
VERSION     DAOMAX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13699_length_2025_cov_4.272589"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK742_13270"
     CDS             <1..712
                     /locus_tag="PK742_13270"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQU77609.1"
                     /translation="LTQTEIGVVSIFVNPTQFNDTADLEKYPRTPGADIGLLEAAGCQ
                     VLYLPDATDVYDGRETATADLGGLDAILEGASRPGHFAGVAQVVGILLDIVQPDKLFM
                     GQKDYQQIQVVNRMLAQRGDGVVLVMCPIVREENGLAMSSRNRRLSAEGRERAGQIYR
                     TLQKAAREAVQKELPVIELESREHLDSQPDFTVDYFSFRHPQTLEPLRHPPADGRMVL
                     VTAVLVEGVRLLDNVLVV"
     gene            1147..1497
                     /locus_tag="PK742_13275"
     CDS             1147..1497
                     /locus_tag="PK742_13275"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQU77610.1"
                     /translation="MFIEVLKSKIHRITVTEANLNYIGSITLDEDLMDAADIVEYEKV
                     QILNINNGERIETYVIKGARGSGVVCLNGPAARRVQFGDVIIVCAYCTMTPEEARSHE
                     PTLVFPDTATNSLS"
     gene            1502..>2025
                     /locus_tag="PK742_13280"
     CDS             1502..>2025
                     /locus_tag="PK742_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQU77611.1"
                     /translation="MLKRIVFNILKFSLFLGIGAALLWVITKDLTEQDRNDIAESFRE
                     ANYWWVVLSVVIGIISHIFRALRWQMMLKPLGHEPRLITTFASVMVAYLANLAVPRLG
                     EVTRCGIMQRYEKVPFDKAVGTIVVERGIDLLSLFIVTILALSLQFSLIAGFFNEKVL
                     LPMEGKFAFSTGTIV"
BASE COUNT          485 a          542 c          530 g          468 t
ORIGIN      
        1 tctcacgcaa acagagatcg gggtggtgag cattttcgtt aatcccaccc agttcaacga
       61 taccgccgat ctggaaaaat acccccgcac cccgggagct gatatcggcc tcctggaagc
      121 agctggctgc caggtgctct acctgcccga tgccaccgat gtctatgacg gacgggaaac
      181 tgctacggct gatctgggcg gactggacgc catcctggaa ggagctagcc gtcccgggca
      241 ttttgcaggc gtagcccagg tggtggggat attgctggat atcgtgcagc ccgataagct
      301 gttcatgggc cagaaggatt accagcagat ccaggtggtg aaccgcatgc tggctcagcg
      361 tggcgatggc gttgtactgg tgatgtgccc catcgtgcgg gaagagaacg gcctggccat
      421 gagctcccgc aaccggcgat tgagtgccga aggcagggaa agggctggtc agatctatag
      481 aacactgcaa aaagcggcca gggaagcagt acagaaagaa ttacctgtca ttgaactgga
      541 aagcagggaa cacctggact cccaacctga ttttacggtc gattatttct cattcagaca
      601 cccgcaaacc ctggaacccc tgcgccatcc accggcagat ggcaggatgg tgctggttac
      661 agccgtgctg gtggaaggtg ttcgcctgct ggataatgtg ctggtggtat agcaggatac
      721 tctttgttga tcatcaactt tgattctcac tgatgtgtga atgcacaatt ccctaaactg
      781 tcttcccggc ccccgagccg ggacctcatc ttctatccga aaagtaagga agccccttct
      841 tgatttgtac acaataccaa cggtcaggtt caatcaaagc acatgatgca ggattttcgt
      901 cataaatcat aactgcatgt atttcagttt tctgcttacc cgtatagaaa actgtgattg
      961 gtatttccgg cacccgatca taagatggag gattggttga tattattact taacaggaga
     1021 tcccggctcg ggggccggga aaacaggata attttaagtt gacccacata attaagtgta
     1081 tcagcaatga tttagccttg ccccgatcac tatcggggtg tctgctataa accctacctt
     1141 tgcaccatgt ttattgaagt gctgaagagc aagatccacc ggatcaccgt aaccgaagcc
     1201 aacctcaact acattggcag catcaccctc gatgaagacc tgatggatgc agctgatatc
     1261 gtcgaatatg agaaggtcca gatcctgaac atcaacaacg gcgagcgtat cgagacctat
     1321 gtgatcaaag gcgcccgtgg ttccggtgtg gtctgcctga acggaccggc agcccgcagg
     1381 gtccagttcg gcgatgtgat catcgtttgc gcctattgta ccatgacccc cgaagaagcc
     1441 cgttcacacg aacctaccct ggtcttcccg gataccgcta ccaactccct ctcctgatcc
     1501 agtgctgaaa cgcatcgtct tcaacatcct gaagttctcc ctcttcctgg ggataggtgc
     1561 tgctttacta tgggtcatca ccaaagacct gaccgaacaa gacaggaatg atatagcaga
     1621 aagtttccgc gaagccaact actggtgggt ggtattatcg gtcgtgatcg gtatcatcag
     1681 ccacattttc agggccctga ggtggcagat gatgctcaaa cccctgggtc atgaaccacg
     1741 ccttatcacc accttcgcct ctgtcatggt agcctacctg gccaacctgg cagtgccgag
     1801 gctgggtgag gtgacccgct gcggcatcat gcagcgatat gaaaaggtgc ccttcgacaa
     1861 ggctgtgggt accatcgttg tggaaagggg catcgacttg ctgagtctgt tcatcgtgac
     1921 catcctcgcc ctgagcctgc agttcagcct catcgccggt ttcttcaatg aaaaggtgct
     1981 gcttcccatg gaaggaaaat tcgccttcag caccggaacc atcgt
//
LOCUS       DAOMAX010000401         2006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_13930_length_2006_cov_2.944131, whole genome shotgun sequence.
ACCESSION   DAOMAX010000401 DAOMAX010000000
VERSION     DAOMAX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13930_length_2006_cov_2.944131"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PK742_13285"
     CDS             <1..1127
                     /locus_tag="PK742_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQU77612.1"
                     /translation="WICSGWLLTGLWSCGQPSAATEVKVGESIPVEYARGLLLESLEN
                     YTHVIVRHPQDTDQVLAEYYLYRTDSVPVSIQDKQGLIHVPVEKIVCLSTTQVGMLEV
                     LDATDRIIGISGTQWVYNEQVSTLIEQGQIREIGQESGLNKELLIQMQPDVILGYASG
                     IAGYDEVDGLSQLGLPVVLIQEFTELHPLGMAEWVKLVALLLDEPAAGDAYFQQVANR
                     YISLTVKAGMLSSLPTVFTGLPYRGEWTVSGGQSFAAQFIRDAGGNYIWADNERTGNF
                     PVSLEEVLLKAADATIWVNPGGASGIQDIIREDERLKHLSAVQTGRVANNNKRLNQAG
                     GNDYWESAVVHPDDVLYDLMQIFHPQHFGDTAMIYYQWLP"
     gene            1124..>2006
                     /locus_tag="PK742_13290"
     CDS             1124..>2006
                     /locus_tag="PK742_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQU77613.1"
                     /translation="MRSYPYKISGLIILIGILFLADLLIGSVYISFQNIWNALLSGNT
                     GADPVSVIVWQTRLPRILAAVLCGLALPLAGLLMQTFFRNPVAGPDMLGITAGAGFAV
                     AMVTMGLGAASLSLVGNMTMITAAVAGAIAVLVLMLLVAGRLRDHISLLILGLMLGTA
                     ISAITGILQYFSNREALKRFIMWTFGSLGGVTWEQLAWMFPFILIGIIVAWSMTRSLN
                     LLLAGETYAVSMGLPLRKTRWLLILVAGLLTGTITAFCGPIAFIGIAVPHVARLIFRT
                     TDHRLLIPGSMLAGVCIM"
BASE COUNT          450 a          470 c          572 g          514 t
ORIGIN      
        1 tttggatctg tagcggatgg ttgctgacag ggctctggtc ctgcgggcag ccatctgctg
       61 caactgaagt taaggtagga gagagcattc cggtggaata tgcccgtggg ttgctgctgg
      121 aatcattgga gaactatacc catgtgatcg tgcggcatcc gcaggacacc gatcaggtac
      181 tggcggaata ctatctgtac aggacggaca gtgtgccggt ttccattcag gacaaacagg
      241 gtttgatcca tgtacctgtt gagaagatcg tttgcctgag caccacccag gtgggtatgc
      301 tggaagtgct ggatgccacg gatcgcatca ttggtataag cggcacccaa tgggtgtata
      361 acgagcaggt aagcaccctc atagagcaag ggcagatcag agagataggt caggagagtg
      421 gattgaacaa agaactcctc atacagatgc agcccgacgt gatcctggga tatgccagcg
      481 gcattgccgg gtatgatgaa gtggatggac tgtctcagct cggtttgcct gtcgtactca
      541 tacaggagtt cacagaatta catccgctgg gcatggccga atgggtgaag ctggtagccc
      601 tgctgctgga tgaacccgca gccggcgatg cctatttcca gcaagtagca aacaggtata
      661 tctcacttac tgtaaaagct ggcatgctgt catctctgcc aaccgtcttc accggtcttc
      721 cttacagggg ggaatggacc gtgagtggcg gacaaagttt tgcggcacaa tttatcagag
      781 atgccggagg aaattacatc tgggctgata atgaacgcac cggaaatttt ccagtgtctc
      841 tggaagaggt attgctgaaa gcagctgatg cgaccatttg ggttaatccc gggggagcat
      901 ccggcataca ggatatcatc cgggaggatg agagactaaa gcatttatca gctgttcaaa
      961 caggccgggt agccaacaac aacaaacgct tgaatcaggc aggcggaaat gattactggg
     1021 aatctgcagt ggtgcatccg gatgatgtcc tttacgatct gatgcagata tttcatccgc
     1081 agcatttcgg ggataccgcc atgatatatt accaatggtt gccatgaggt cgtatcctta
     1141 caaaatatca ggtctgatca ttcttattgg tattttattt ctggcagatc tgttgatagg
     1201 ttctgtgtat atttcttttc agaatatatg gaatgcgtta ctgagcggaa atactggtgc
     1261 tgatccggtt tctgtcatcg tctggcaaac cagattgccc cgtatcctgg cagctgtatt
     1321 gtgcgggctt gcattgccac ttgccggatt gctcatgcaa acattcttcc ggaacccggt
     1381 ggcaggtcct gatatgctgg gcatcacggc cggggcagga ttcgccgtag caatggtcac
     1441 tatggggcta ggcgctgctt ctttgtcgtt ggtgggaaac atgaccatga tcacagccgc
     1501 agtggccgga gccatcgctg tcctggtcct gatgttgctt gttgccggaa gattacggga
     1561 tcatatcagc ctgcttatcc ttggcctgat gctgggcaca gccatttctg ccatcaccgg
     1621 gatactacag tatttcagca atcgtgaagc actcaagcga ttcatcatgt ggaccttcgg
     1681 cagcctggga ggtgttacct gggagcagct tgcctggatg tttccgttca ttctgatcgg
     1741 catcatcgtg gcctggtcca tgacccggtc gctcaacctc ttgctggccg gagagaccta
     1801 tgcagtgagc atgggtttgc cgcttcgaaa gaccaggtgg ctgctgatac tggttgcggg
     1861 tttgctgacc ggaaccatca ctgcattctg cggaccgatc gccttcattg gcattgcggt
     1921 tccgcatgtg gcgaggctga tcttccgaac cacagatcat cgattattga tacccggttc
     1981 catgttggct ggtgtgtgca tcatgt
//
LOCUS       DAOMAX010000402         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14038_length_1998_cov_5.005661, whole genome shotgun sequence.
ACCESSION   DAOMAX010000402 DAOMAX010000000
VERSION     DAOMAX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14038_length_1998_cov_5.005661"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1169
                     /gene="cydB"
                     /locus_tag="PK742_13295"
     CDS             135..1169
                     /gene="cydB"
                     /locus_tag="PK742_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQU77614.1"
                     /translation="MATFLGIDYPTLWFLLVGALFSGYAILDGFDLGAGAWHLFFQKE
                     ESRRIALNAVGPVWDGNEVWLVIGGGALFAGFPMVYATLFSAMYIPFMLFLVFIILRA
                     VSIEFRSKESMKWWRRMWDIAYSVSSILLAVLLGVVLGNVLQGMPLDADHEFSGHWLQ
                     FLNPYALLTGITTLALMMLHGALYLSMKTEGRLFVKIVILMRRSIIAFLVLFSLVTLY
                     TLLYIPHLTDRFREEPALFVAPLLAVLSIANIPRLVSKRKYPMAFLFSSLVFAFMLII
                     VAIELYPVLVIATNDAANSLTIYNSASSEKSLGIMLTIAAIGAPLVGIYTIIVYRTFR
                     GKVKMDDHSY"
     gene            1221..>1998
                     /locus_tag="PK742_13300"
     CDS             1221..>1998
                     /locus_tag="PK742_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQU77615.1"
                     /translation="MRPVAFLLCCCFFVTVQAQTSYYVSPDGSNAGDGSMASPWLTVQ
                     FGLEHIVPGDTLNLREGIYTEKIAIPFSGITLRSMPGETAVIDAGIMAPQDAAIEIYH
                     ISDITIEGLEIRNNVRNNAQGILIEGYGDHFTIRNCVIHDIHFSADPDDNATPSKNAQ
                     GIIVYGTHPSIPITNLLITDNEVYNCRLGYSEGIAVNGNVDGFEVSHNYVHDLTNIGI
                     DIIGHEGTCSTDSLDQARNGKVLRNTVHDCLSAYATSGGLY"
BASE COUNT          475 a          499 c          494 g          530 t
ORIGIN      
        1 tatagtgctg ttcctgctgt ttatctatct gctgaataaa aagatccaac gcggaccgga
       61 agcagagggg atgatagaac acaggcccaa gctgcaggaa atgagtaaca ctttcggagg
      121 taaaaacaac tgacatggcc acattcctcg gtatagatta cccaacgctt tggttcctgt
      181 tggtaggagc actgttctcc ggttatgcca tcctggatgg tttcgatctg ggtgcaggag
      241 cctggcatct gttcttccaa aaagaagaaa gtcgtcgcat tgcccttaat gcggtcggtc
      301 cggtatggga tggcaatgag gtctggctgg tcattggtgg tggcgccttg tttgccggtt
      361 tcccaatggt ctatgccacc ctgttcagtg ccatgtatat accctttatg ttgttcctgg
      421 tgttcatcat cctccgggcg gtgagcatag aattcagaag caaggaatcc atgaaatggt
      481 ggcgcaggat gtgggatatt gcctatagtg tatccagtat tctccttgcc gtcttgctgg
      541 gtgtcgtgct gggcaatgta ttgcagggca tgccactgga tgccgatcat gaattcagcg
      601 gacactggct gcaattcctc aatccctatg ccctgctgac aggcatcacc accctggcgc
      661 tgatgatgct gcacggagca ctctacctga gtatgaagac agaaggcagg ttattcgtga
      721 agatcgtcat cctgatgcgc cggtctatca ttgcttttct ggtgttgttc tcactggtca
      781 ccttatatac gttgctgtac atcccacacc tgaccgacag gttccgggag gagccggctt
      841 tatttgtcgc tcccttgctg gctgttctga gcatagccaa tattccgcgg ttggtgagca
      901 aacggaaata cccgatggca ttcctgttct cttcgctggt ctttgccttt atgctcatca
      961 ttgtggctat tgagttatat ccggttctgg tgatcgctac caacgacgcc gccaacagcc
     1021 tgaccattta caattcagcc agttcagaaa aatcgctggg tatcatgctc accatcgcag
     1081 ccataggcgc accgttggtg ggtatataca ccatcatcgt ataccgtact ttccgcggaa
     1141 aggttaaaat ggacgaccac agctactgat cctcacagtg actgttttat acgaactttg
     1201 ggtaaacctt ccattcttct atgcgacctg ttgcatttct gttatgctgt tgtttcttcg
     1261 tcactgtgca agcccagacc agctactacg tatctcctga tggcagtaat gccggtgatg
     1321 gcagcatggc atcaccctgg ctaaccgtac agttcgggct ggaacatatt gtaccgggag
     1381 acaccctcaa cctgcgtgaa gggatttaca ccgaaaagat tgccatcccc ttttccggta
     1441 tcaccctccg gagtatgccg ggcgaaaccg cggtcattga tgccgggatc atggcaccac
     1501 aggatgctgc catagagatc tatcacatat ctgatattac tattgaagga ctggagatcc
     1561 ggaataatgt acgcaacaac gcacagggaa tactgataga aggctatggc gatcatttta
     1621 ccatccgcaa ttgtgtgata cacgacattc acttctcagc tgatcccgat gacaatgcca
     1681 ctccctcaaa aaatgcacaa ggcatcattg tctatggaac acacccgtcc attcccatca
     1741 ccaacctgct gataacagac aatgaggtgt ataactgcag gctggggtac agcgaaggca
     1801 ttgcagtgaa tggcaatgta gatggtttcg aggtaagcca taattatgta catgacctga
     1861 ccaatatcgg gatagatatc atcggccatg aaggcacctg ctccacagat tcactggatc
     1921 aggcaagaaa cggaaaggtg ctgcgcaata ccgttcacga ttgcttgtct gcctatgcta
     1981 ccagcggtgg tttgtatg
//
LOCUS       DAOMAX010000403         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14052_length_1997_cov_3.934089, whole genome shotgun sequence.
ACCESSION   DAOMAX010000403 DAOMAX010000000
VERSION     DAOMAX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14052_length_1997_cov_3.934089"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1262)
                     /gene="lpxK"
                     /locus_tag="PK742_13305"
     CDS             complement(219..1262)
                     /gene="lpxK"
                     /locus_tag="PK742_13305"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQU77616.1"
                     /translation="MRLLLYPLSWLYGLVVGLRNFFYDRGWLRSFQFDFPVVLVGNLS
                     TGGTGKTPHVLAIARILSSKYRVAVLSRGYKRKTRGYIASTELSLVEDIGDEPKLMKQ
                     RMPELEVAVCENRLMGISELLQEEPPPNIILMDDGFQHRKVRAGLNIILTDYSKLFTK
                     DHLLPVGRLREPRSSLRRADVIIVTKCPPDMSKREAAEMRTTLHLLPLHKLFFTAYKY
                     APLKPLLPDQMQTEPGGGHGFLVVCALASNQYLKDHLQSIRPDVKELCFPDHHYYKAA
                     DMQKIKQQLDGDRLLVTTEKDAVKLMEIADDIRAAGLSFFVLPVEVEFLFEKAEKFAT
                     LIDRYVKDAKSVI"
     gene            complement(1338..1838)
                     /locus_tag="PK742_13310"
     CDS             complement(1338..1838)
                     /locus_tag="PK742_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77617.1"
                     /translation="MRWHALFVLFMLVCVGSESHAQLDTVMVYNVHCSFSDTTGDTVN
                     FYNWGGSWTSFFPTEECVEPKEKYGRNRDTVMTYVQADKPFWFMLYDTKDHLLYEGLH
                     YRNCTVGPFIVYHPNGKVRMRGQYSGAKWKDDHFKVTDCEGKPTGKWISYNLIGNIVE
                     TRDYGK"
BASE COUNT          502 a          506 c          500 g          489 t
ORIGIN      
        1 taccctggcg cctttgcgtt tccgttttaa cgcaaaggca caaaggaggc gcaaaggaca
       61 cgaaggacct agacaaaaga tcaaactcaa taatggaatg tctctccggc ctccgagccg
      121 gagtctcctt cacagaacga aggagggtat tccctgcctg ccggcaggca ggcgggtcaa
      181 gcccggaaag acagtaactg agatgttagt caactagatc agatcacaga cttcgcatct
      241 ttgacatacc gatcgatcaa ggttgcgaac ttctctgctt tctcaaagag gaactccacc
      301 tccaccggca atacaaagaa ggacaatccc gcagccctga tatcatccgc gatctccatg
      361 agtttgaccg catccttttc agtagtgacc agcagacgat ccccatcgag ctgctgtttg
      421 atcttctgca tatctgcagc cttgtagtag tgatgatccg ggaagcagag ctccttcaca
      481 tccggacgaa tgctttgcag gtgatctttc aggtactggt tggaagccag ggcacagacc
      541 accagaaatc cgtgtccgcc gcccggttct gtttgcatct gatcgggcag caaaggcttt
      601 agcggagcgt acttatatgc tgtaaagaag agcttgtgca atggaagtaa atgcagtgtg
      661 gtacgcatct ctgccgcttc ccttttactc atgtcgggcg gacatttggt cacgatgatg
      721 acatcggcac ggcgcaggga ggaacgcggt tcacgaagtc ttccaacggg taacaggtga
      781 tctttggtga acagtttgct gtaatcggtc aggatgatgt tgagtccggc cctgaccttt
      841 cggtgctgga agccatcgtc catcaggatg atattgggag gcggttcttc ctgcagcagt
      901 tcgctgatac ccatcagcct gttctcacac acagctactt ccagttccgg catccgctgc
      961 ttcatcagtt tgggttcatc gccgatatct tctacgaggc tgagctctgt actggcgata
     1021 taccctctgg tctttcgctt atagcctcgg ctcagtaccg caaccctgta tttgctgctc
     1081 aggatgcgtg cgatggccag cacatgcggg gtctttccgg tacctcctgt actcagattg
     1141 cctaccagaa ccaccggaaa atcaaactgg aaactgcgca gccatcccct gtcgtagaaa
     1201 aagttgcgca agcctacgac caggccgtac agccaggaga gcgggtaaag gaggagacgc
     1261 acccgacgaa gttactgaaa actaatggat tagaaacttc tgttacagaa ttacaaaaaa
     1321 cttaaaaagc cattaaccta cttgccgtag tcacgggtct ccacgatgtt gccaatgagg
     1381 ttataggaaa tccatttgcc ggttggcttt ccttcacagt cggtcacctt aaagtgatcg
     1441 tctttccatt tggcacctga gtattgtcca cgcatacgca cttttccatt gggatgatag
     1501 actatgaagg ggcccaccgt gcagttgcgg taatgcagtc cttcgtagag cagatgatct
     1561 ttggtgtcat acagcatgaa ccagaaaggt ttatctgcct gtacataggt catgacggta
     1621 tctctgttcc tgccgtattt ctctttaggt tccacgcatt cttctgtggg gaagaaggat
     1681 gtccaactgc caccccagtt gtagaagttg acggtatcgc ctgtggtgtc gctgaacgaa
     1741 cagtgtacgt tatacaccat gacggtatcc agctgggcgt gcgattcaga gccaacacaa
     1801 accagcatga acagaacgaa aagtgcatgc caacgcatgg agtaaatgta cagaagcaga
     1861 aacagacatt gatttaagta aatgtcacaa atgggtggag gcgtatggtt ttttgaatgg
     1921 tgatcatcta cctaaaaatg cttctccggg cttgacccgg agtctccttc atagaatgaa
     1981 agggagatcc cccaaac
//
LOCUS       DAOMAX010000404         1989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14143_length_1989_cov_4.097725, whole genome shotgun sequence.
ACCESSION   DAOMAX010000404 DAOMAX010000000
VERSION     DAOMAX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14143_length_1989_cov_4.097725"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1989
                     /locus_tag="PK742_13315"
     CDS             <1..>1989
                     /locus_tag="PK742_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQU77618.1"
                     /translation="ERYGIPLLFLDPEYLGHVSFLSYFILGVAFGAYFMVWNVTSYIL
                     HARHFPFLATLQRPFGVYSLNNSLIPLAFLIAYLVQLLIFQRQDGLQDWGIIALRTIG
                     LLCGIILFISISMAYFFSTNKNVFQLFRLDPTQHDLQDLPFEGKTWQMAGQPHSIHID
                     SYINHQLSVKLARKAGHYPAYIIEKVYRQHHVNALFIELTSLLLLLVLGHLIDYPVFR
                     IPAASSILLLFAIGLMLIGAVSYWLKGWKVLISVLAIILLDILIGHGLLQYRNQAFGI
                     DPKQTGAVYDLESLQQLHDPARLEADKQHMLQVLNNWRAKFPEDAPPKMIFVNCSGGG
                     LRATMFVMDALQKADSITNGQLMQHSMMMTGASGGMIAAAYYRELYRMQIAGYSVNRY
                     DDLYLQKISSDLLNAIAYTMVVNDLFFPWQSYTHEGISYRKDRGYMFEKVLHENTDSL
                     LFHPVSSSYQDEYEARIPLLLMYPTIVNDERKLFIGAQPFSYLGIPAERLKDDRTPEY
                     DGIDIRQLLGNEQADRLLLSSALRMSCTFPYILPNVYLPTDPQIEVMDAGIRDNYGIE
                     SSVRFATTFRHWIDSNTSGIVVLNIRGLENSMPIRENVSSGILEKVISPIGNLYLNWV
                     EVQDYQHDLLLHYLEEIMQTPVDVLTIAYEPAPGSRRAS"
BASE COUNT          493 a          502 c          470 g          524 t
ORIGIN      
        1 gaacgatatg gcattccgct tctgttcctg gatcccgagt atctgggtca tgtcagcttc
       61 ctgtcttatt tcattctggg tgtagctttc ggagcctatt ttatggtgtg gaacgtaacc
      121 agttacatcc tgcatgcacg gcactttcca ttcctggcca ccctgcaacg gccctttggt
      181 gtgtacagcc tgaacaacag cctgataccc ttggctttcc tgatcgccta cctggttcag
      241 ctactgatct tccagcggca ggatggtctt caggattggg gcattattgc cctgcgtact
      301 atcggattgc tatgcggtat catcctgttc atcagcatca gcatggccta tttcttcagc
      361 accaataaga atgtatttca gctcttccgg ctcgatccaa ctcaacatga tctgcaggat
      421 cttccatttg aaggaaaaac ctggcaaatg gccggccagc cgcacagcat tcacatagat
      481 tcatatatca atcaccagct cagcgtcaag ctggcccgta aagcagggca ttatccggcc
      541 tatatcatcg aaaaggtgta ccggcagcat catgtgaatg ctttgttcat tgaactaact
      601 tccttgctat tgctgctggt gcttggacat ctgatcgatt atcctgtctt cagaataccg
      661 gcagcttctt ctatcctcct gttatttgcc atcgggctta tgctgatcgg tgcggtcagt
      721 tactggctga agggctggaa ggtactgatc agtgtactgg ccatcatcct gctcgatatt
      781 ctaattggac acggactgct gcaataccga aaccaggcct ttggtattga cccgaaacaa
      841 acgggagccg tctatgacct ggaaagttta cagcagctgc atgaccctgc cagactggaa
      901 gcagacaagc aacacatgct gcaggtgctg aacaactggc gtgcgaagtt tccggaagat
      961 gccccgccta aaatgatctt cgtgaattgt agtggcggag gattgcgggc aaccatgttc
     1021 gtgatggatg ctttgcagaa agccgatagc atcaccaacg gacaactcat gcaacacagc
     1081 atgatgatga caggcgcatc gggcggaatg atagccgcag cctactatcg tgaattgtac
     1141 agaatgcaaa ttgcaggcta ctccgtcaac cggtatgatg acctgtatct gcaaaagatc
     1201 tcttcagacc tgctcaatgc cattgcctac accatggtgg tcaatgacct gttctttccc
     1261 tggcagtcat acacccatga aggtatcagc taccggaagg atcgcggata catgttcgaa
     1321 aaagtgctgc atgagaatac agattctctc ttgtttcatc ctgtttcgtc cagctaccag
     1381 gatgagtatg aggcccgcat tccgttgctg ctcatgtacc ctaccattgt gaatgatgaa
     1441 cgaaaactgt tcatcggtgc acaacccttc agctacctgg gcattccggc tgaaagactg
     1501 aaagatgacc gaacaccgga atacgacggc attgacatca gacaacttct gggcaatgaa
     1561 caggcagacc gtttattgtt atcaagtgcc ttgcgcatga gctgcacctt cccctatatc
     1621 ctgcccaacg tttatttgcc aaccgatccg cagatcgaag tgatggacgc cggtatcaga
     1681 gataattacg gcattgaatc atctgttcgt tttgccacta ccttccggca ctggatagac
     1741 agcaatacaa gtggtattgt agtattaaat atacgtggtt tggaaaattc catgcctatt
     1801 cgtgagaatg taagcagcgg tattctggaa aaggtcatca gtcctattgg caacctgtat
     1861 ctgaactggg tggaagtaca ggattaccag catgatcttt tactgcacta tctggaagag
     1921 atcatgcaga cacctgttga tgtactgacc attgcatatg agccagcacc gggcagcagg
     1981 cgggcttca
//
LOCUS       DAOMAX010000405         1988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14157_length_1988_cov_3.066218, whole genome shotgun sequence.
ACCESSION   DAOMAX010000405 DAOMAX010000000
VERSION     DAOMAX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14157_length_1988_cov_3.066218"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PK742_13320"
     CDS             complement(<1..600)
                     /locus_tag="PK742_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQU77619.1"
                     /translation="MRNTWSLIGLLLLFAGCGSNKDAADSPRVFRLNLSAPLTSLDPA
                     FASDQPNSWACLQLYNGLVQLDRNLQVVPSIAGSWEIGDDQKTYTFHLRQDVFFHDSE
                     CFPNNTGRKVRAADFVYSFSRLSDPATAARGFWVFDQLLDEDAPFLAPNDSTLVIRLR
                     APFAPFLQRLCMPYCSVVAEEAIVAYGKDVRSHPVGTGPF"
     gene            complement(602..1126)
                     /locus_tag="PK742_13325"
     CDS             complement(602..1126)
                     /locus_tag="PK742_13325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQU77620.1"
                     /translation="MITIISATNRKDSNTLKVAKEYERLIREKGREVLLLNLESLPVS
                     VMSAAIYEEDDVDFSAIQEQYLFPADKFIFVMPEYNGSMPGVLKMMIDVADVERAFYG
                     KKALMVGIATGRAGNLRGMDHLTGVLNHMKMSVHYNKLPISRVTHELDAEGRLSNTTT
                     RLVVEKQIDEFLYF"
     gene            1275..1955
                     /locus_tag="PK742_13330"
     CDS             1275..1955
                     /locus_tag="PK742_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQU77621.1"
                     /translation="MQVTYYGHSCFGITLQGARILFDPFISPNKLASAVRVDDISADF
                     ILISHGHEDHMADAVSIAGRTGATIIGAFELTSWMEKQGVSSVHPMNIGGQKMFPFGK
                     VKMVNAVHSSTLPDGTPAGPAAGFLIQTPEITFYYAGDTALHYDMKLLGKHYKVDLAF
                     LPVGDNFTMGAEDAVIASRYIKCDRIVGMHYDTFGYITINHDEARKHFENEDKTLYLL
                     PIGETTNF"
BASE COUNT          544 a          484 c          461 g          499 t
ORIGIN      
        1 gaagggaccg gtaccaaccg gatggcttcg aacatctttt ccataagcaa caatggcttc
       61 ttctgctact acagaacaat agggcatgca aagacgctgg aggaaggggg caaaaggcgc
      121 cctgagccgg atcaccagcg tgctgtcatt cggggccaga aacggggcat cttcatcgag
      181 cagctgatcg aacacccaaa agccccgggc agcagtggcc ggatcagaca acctgctgaa
      241 actatacacg aaatctgccg cacgcacttt tctgcctgtg ttgttcggaa aacactcact
      301 gtcatggaaa aaaacatcct gcctgagatg aaaggtgtag gttttctgat catcaccaat
      361 ttcccaggaa ccggcaatgg atggcactac ctggagattc ctgtccagct gcaccaaccc
      421 attatatagt tgcaggcagg cccaggaatt gggctggtct gaggcaaatg ccgggtcgag
      481 tgatgtaagc ggagcactca gattcagtcg aaataccctt ggtgaatcag cagcatcctt
      541 gttgctgccg cagccggcaa acaacagcag caggccaatc aatgaccagg tattgcgcat
      601 atcagaagta caggaattcg tcaatttgct tttctaccac caaccgtgtg gtggtattgc
      661 tgagacgccc ttcagcatcc agttcatggg taaccctgct gataggaagc ttattgtaat
      721 ggacagacat cttcatatga ttcaacacac ctgtcagatg gtccatcccc cgcaggttgc
      781 ctgcacgtcc ggtagcaata ccaaccatca gagccttctt tccgtaaaat gctcgttcca
      841 cgtctgccac atctatcatc atcttcagca caccgggcat actgccatta tattcaggca
      901 tgacaaaaat gaatttatcg gcaggaaaca gatattgctc ctgaatggcg ctgaagtcca
      961 catcgtcctc ttcataaatg gcggcactca tgacagaaac cggaagtgat tccagattga
     1021 gcaaaaggac ctcccggcct ttctcccgga taagcctttc atactctttg gcaaccttca
     1081 gggtgttgct gtcttttctg ttggttgctg aaataatggt gatcatatgc atgcgttaca
     1141 aaaataccgg ctgttatcaa aaccggaaat cattatccac agatacaaat tttcctgtga
     1201 aaaagtttat gatcatttga tttcacaggg catgttgaaa acataccttt acctcagttt
     1261 actggagcag ccatatgcag gtcacttatt acggacattc atgttttggg atcacccttc
     1321 agggtgcccg cattctgttc gatcctttta tttcgcccaa taagctcgca tctgctgtgc
     1381 gggttgatga catttctgcc gatttcatcc tgatcagcca cggacatgaa gaccatatgg
     1441 cagatgccgt ttctattgca ggcagaacag gggctaccat catcggggct tttgaattga
     1501 ccagctggat ggaaaaacag ggtgttagtt cggtacatcc tatgaatatt ggcggacaaa
     1561 agatgttccc cttcggtaaa gtgaagatgg tcaatgctgt gcattccagt actttaccgg
     1621 atggcactcc tgctggtccg gcggcaggct ttctgatcca gacaccggag atcacatttt
     1681 attatgcagg cgatacggcc ttgcattatg acatgaaact gctgggaaag cactacaaag
     1741 tggatctggc attccttcct gttggcgata atttcaccat gggagctgaa gatgcggtca
     1801 tcgccagcag gtatatcaag tgtgaccgga tagttggaat gcactatgat acttttggtt
     1861 acatcacgat aaaccatgat gaagccagga aacattttga gaacgaagat aaaaccttat
     1921 acctcctgcc aataggagaa acaaccaatt tttaatatgg gaaagatcat agcaatagcc
     1981 aatcaaaa
//
LOCUS       DAOMAX010000406         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14180_length_1986_cov_2.958571, whole genome shotgun sequence.
ACCESSION   DAOMAX010000406 DAOMAX010000000
VERSION     DAOMAX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14180_length_1986_cov_2.958571"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..>1986
                     /locus_tag="PK742_13335"
     CDS             27..>1986
                     /locus_tag="PK742_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77622.1"
                     /translation="MQIKKVFAGVKNTVALRRLMKQCYDVISQEQMDEQMLAQGKHLP
                     KAVPMEHTLPASSARTMANYLQMGWAQIVKDKAKRSPAEKKAEQVMMQYVKDRMNNID
                     ATLKKAAKENWAKKDIDELKEEYRKLDVYRKKESFGVLSLYNQMRVGIASTKYFSKFL
                     DDSNSPSGKLKYAAKQAADSFMRGRGIIESGQYESVETDEFGNRFVYIGNNEETGMPM
                     YRSIDGGQIFFADRLRSADLPKQSISNDFFAALKEELSARGVEVDDRWFGTIDKSTSK
                     SLSEAQVSKYLSKYIEDADQLKDAVNFIMTEAAESIEIQKDEQGFDVYDEDGNPVYDI
                     ESGKPTLRNTIKYLYNTGRIKFLFGSTEAMGVGMNLQYNTTDIHHIDMPQRPRDYVQR
                     NGRSVRQGNKNFDVNVHSYTTQGGPEIIMLQLLKVKDRFISSLFNFNDEETSSDTGEV
                     GDDKMSVLTKQLENLISNTTDTRVSGYVQLKKQLDRTVQGIKDARRATETLSTAFGEA
                     EKLTQNLAVKQKRIQILESVRDWFNQKIDQQEANDKGFKKAYQEWESQNGRKERLTQL
                     NKDLDSGLIDLEKYKDERTKLMETAPKREMVEITAEDMQQLIDDYQMPDFTPSLVSNL
                     AYELLYPGELNTVSYTNKELQSSLFSKGY"
BASE COUNT          621 a          398 c          527 g          440 t
ORIGIN      
        1 aggaaatggc cgggcagaac ggtaaaatgc agataaaaaa ggtatttgct ggggtgaaga
       61 acaccgttgc cctgcgccgt ctgatgaaac agtgctatga cgtgatctcc caggagcaga
      121 tggacgagca aatgttggct cagggcaagc acctgccgaa agctgttcct atggagcata
      181 cacttccggc ttcatcggct cgtaccatgg caaactatct gcagatgggg tgggcgcaaa
      241 ttgtaaagga taaggctaag cgaagccctg cagagaagaa agccgaacag gtgatgatgc
      301 agtatgtcaa ggatcggatg aacaacattg acgccacctt gaagaaagct gcgaaagaga
      361 actgggcgaa gaaagacatt gacgagctga aggaggaata tcgaaaactg gacgtatatc
      421 gtaaaaagga atccttcggg gttctcagcc tttacaacca gatgcgggtt ggcattgcca
      481 gcaccaagta cttcagtaag ttcctggatg atagcaatag cccgagcggt aaactgaaat
      541 atgctgcgaa acaggcggct gacagtttca tgagaggaag aggtattatt gagtctggcc
      601 agtacgaaag tgttgaaacc gacgaattcg gcaacaggtt cgtgtatatc ggcaacaatg
      661 aagaaaccgg catgcctatg taccggtcta ttgatggtgg ccagatattc tttgctgatc
      721 gactgagaag cgccgatctc ccgaagcaaa gtatatccaa tgatttcttc gcagcattga
      781 aggaagagct gagcgctcgt ggggtagaag tagatgatcg gtggtttgga acaatcgata
      841 aaagcacatc aaagagttta tctgaggcgc aggttagcaa atacctgagc aagtacattg
      901 aggatgcgga tcagttgaag gatgctgtca atttcatcat gactgaagcg gccgagtcaa
      961 tagaaataca gaaagacgaa cagggctttg atgtttatga tgaagatggt aatcctgtgt
     1021 atgatattga atccgggaag cccaccttgc gaaacaccat caagtatctg tataatacag
     1081 gcaggatcaa attcttattt ggttcgacag aggcaatggg ggtgggcatg aacctccagt
     1141 acaataccac ggatatccat cacattgata tgcctcagcg tccgcgggat tatgttcagc
     1201 ggaatggccg tagtgtgcgc cagggcaaca agaactttga tgtgaacgtg cactcctaca
     1261 ccactcaggg cggtccggag atcattatgc tccaacttct gaaagtgaag gacaggttta
     1321 tttccagcct gttcaacttc aacgacgaag agacatcttc tgataccgga gaggtcggag
     1381 acgacaaaat gagcgttctc accaaacagc tggagaacct gatcagcaat acaactgaca
     1441 cccgggtgag cggatatgtt caattaaaga agcagctgga caggactgtg cagggtataa
     1501 aggatgcgag acgggcaaca gaaaccctgt ctactgcatt tggagaagcg gaaaagctga
     1561 ctcagaacct tgctgttaag cagaagcgga ttcagatcct tgagtctgta cgggattggt
     1621 tcaatcagaa gattgatcag caagaagcca acgacaaagg cttcaaaaaa gcataccaag
     1681 aatgggagtc tcagaatggt aggaaagaac gtttaactca gctgaacaaa gaccttgatt
     1741 ctggcctcat agatttagag aaatacaagg atgagcgcac gaagctgatg gaaaccgcac
     1801 cgaagcggga aatggtagag atcacagcag aagacatgca gcaattgatc gatgattatc
     1861 aaatgcctga tttcacgcca tcattggtat cgaatcttgc ttatgaatta ttataccctg
     1921 gggaattgaa tactgtttcc tatacaaata aggaattgca atcttcttta ttttccaaag
     1981 ggtata
//
LOCUS       DAOMAX010000407         1976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14298_length_1976_cov_4.819365, whole genome shotgun sequence.
ACCESSION   DAOMAX010000407 DAOMAX010000000
VERSION     DAOMAX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14298_length_1976_cov_4.819365"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>1976)
                     /locus_tag="PK742_13340"
     CDS             complement(44..>1976)
                     /locus_tag="PK742_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQU77623.1"
                     /translation="SRNFWYLVAGALIIGVLAHLAKATFLGNLSIVVALIATLNRFVL
                     TPASIRFQKGPLVRLESFYNRTISYALKGYRPVWFFLGTVLLFLGSCASLGIIPPKVI
                     YFPEGDPTYVNVFIAAPLGTDIQKTNAITEQVESKLKTILADDSDVVEAVLAQVGEGT
                     SDPNEGVSMGSSPHKGRVTVSFVPFEQRNGKSTSRIMEDIREGVKDIPGAQITVNPSP
                     FGPPVGPPINLEVSGEDFTQLILLTEQLREYMEDAGIPGIEELKTDLETGKPELVLHV
                     NRDAAKRYGVSTADVAMSLRTALFGKEVSKYKEGEDDYPIMLRFEDDKRYNLSTLLNT
                     RITFRDQATGKISQVPVSAVADLDYSSTYGSIKRKDLDRVITIYSNVNVGYNANEINA
                     QLKERLEAFDMPEGFTYKFTGQQEDQASDMAFLTNALIIAVLLIFLIIVSQFNSVLMP
                     LIIMSSVVFSMIGVLLGIQIFQMDIVILMTGVGIISLAGVVVNNAIVLIDFIELERNR
                     RALELDLENPEHLPYDELLMSIVKAGSTRMRPVLLTAITTVLGLIPLAMGININFFTL
                     LSDFDPQYYVGGDSVMFWGPLAWTVIFGLTFATFLTLVIVPVTYLLIDRVKMRVLRAT
                     GSMPKEQKPPVGALDADSL"
BASE COUNT          493 a          508 c          504 g          471 t
ORIGIN      
        1 aaaaagaccc gctttcgcgg gcctttctat cagataagaa ccattataaa ctgtcggcat
       61 ccagggcacc aaccggaggc ttctgctctt tgggcatgct gccggtggca cgcaataccc
      121 gcatcttgac acgatcaatg agcaggtaag tgaccggtac aatgaccagg gtgaggaagg
      181 tggcaaaggt cagaccgaag atcacggtcc aggccagggg cccccagaac atcacactgt
      241 cgccgcccac atagtactgc ggatcgaaat cactcagcag ggtgaagaag ttgatgttga
      301 tacccattgc aagaggaata agtccgagca cggtggtgat ggcagttagc aacaccggtc
      361 gcatacgggt ggaacctgcc tttacaatgg acatcagcag ttcatcatac ggcaggtgtt
      421 ccggattttc caggtcgagt tctagtgctc ttctgttcct ttccagttct atgaaatcta
      481 tcaggacgat ggcattgttc actaccacac ctgccagcga aatgataccc acacccgtca
      541 tcaggatcac aatgtccatc tggaatatct ggatacccaa caacacaccg atcatggaaa
      601 ataccacaga agacatgatg atgagtggca tcaacacgga gttgaactga gatacgatga
      661 tgaggaaaat gagcagcact gctatgatca gcgcattggt cagaaaggcc atatcagatg
      721 cctgatcttc ctgctgtccc gtgaatttat aagtaaagcc ttccggcata tcaaaagctt
      781 ccagtctttc tttcagctgg gcgttgatct cattggcatt gtaacccaca ttcacgttgg
      841 agtaaatggt gatgacccgg tccaggtcct tgcgtttgat acttccgtaa gtggaagagt
      901 aatccaggtc agctacagcc gacacgggta cctggctgat cttaccggtg gcctgatcgc
      961 ggaaggtgat gcgggtattc aacaaagtac tgaggttgta ccgcttatcg tcttcaaagc
     1021 gcagcatgat gggataatca tcttcacctt ccttgtactt cgacacttcc ttgccgaaca
     1081 acgccgtacg caatgacatg gccacatctg cagtactcac gccatatcgc ttggcagcat
     1141 cgcggttcac atggagcacc agttcaggct tgcctgtttc cagatcggtc ttgagttctt
     1201 cgataccggg tatgccggca tcttccatgt actcacgcag ttgttcggta agcaggatga
     1261 gctgggtgaa gtcctcgccg gatacttcca ggttgatggg tggacccacc ggcggaccaa
     1321 aaggcgatgg attcaccgta atctgtgcgc cgggaatgtc tttcacgcct tcacgaatgt
     1381 cttccatgat gcgactggtg cttttgccat tccgttgttc gaagggaacg aaggaaacgg
     1441 tgacacgtcc tttatgtggc gagctgccca tgctgacccc ttcgttggga tcagaagtgc
     1501 cttcgcccac ctgagcgagt acagcttcca ctacatccga atcatcggcc aggatggtct
     1561 tgagtttact ttccacctgt tcggttatcg catttgtttt ttgtatatca gttcccaatg
     1621 gagccgctat gaatacgttc acataagtgg ggtctccttc cgggaaataa atgaccttcg
     1681 gcggaatgat gcccaggctg gcacaacttc cgagaaacag caacacagtg cccagaaaga
     1741 accaaactgg tctgtaacct ttcagcgcat agctgatggt gcggttatag aaactttcca
     1801 gtcgtaccag cggacctttc tggaatctga tggaagcagg ggtcagcacg aagcggttca
     1861 gagtagctat gagtgctact acaatagaca ggttacccag gaaggtggct ttggccagat
     1921 gcgccagcac tccgatgatc agcgcacccg ccacgaggta ccagaaattc ctggaa
//
LOCUS       DAOMAX010000408         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14490_length_1960_cov_3.788451, whole genome shotgun sequence.
ACCESSION   DAOMAX010000408 DAOMAX010000000
VERSION     DAOMAX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14490_length_1960_cov_3.788451"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PK742_13345"
     CDS             <1..465
                     /locus_tag="PK742_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77624.1"
                     /translation="FYYKLGFRPDTAALRQMAISEQKKIVLRKGYRSSEKTLLKFTES
                     NISLKGKGPVQQGVYDITSKVKQMITRKYQGNSTEAEEACVSAFLAKTGRQDAFTKEE
                     TEVLKQVSLWAAAMNITDKKQLHLLLETVKLKPVDEYRYQATLRQFFGDKYQ"
     gene            complement(475..1122)
                     /locus_tag="PK742_13350"
     CDS             complement(475..1122)
                     /locus_tag="PK742_13350"
                     /inference="COORDINATES: protein motif:HMM:NF039455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HQU77625.1"
                     /translation="MSSKLVYPRFVMVKDDFYDDPMEVYQAAMSADYYEPRYYTGLRS
                     RTVYHQPGVKRKLERILGIKITRFDTDPLDENGVFYCGYAKGNKKEVPGVHYDHPPED
                     ITVVIYLTPDLPFDCGTSLWMHKKTGITDCPTAADARNLNMKLSDLRQLFEDDAKKRS
                     KWQEIDRVGYRFNRMVAYPSGVMHSATNHFGGSISDVRLYQTFRIGVDWSTFRMK"
     gene            1257..>1960
                     /locus_tag="PK742_13355"
     CDS             1257..>1960
                     /locus_tag="PK742_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77626.1"
                     /translation="MRSATSHFLSRLRTGMYGLLILLSVSLSAQPNAPIWPTDVQYKF
                     NIGTYTWTDSNSYQFMVLQVTGLPDINTEYSKIIELELSLSDQPAFTWQQDPYSGGYG
                     YVSLISKDQTFAPVMVLKEVVYDSVSGFHQIQHTSLFLTDDFDRIKQDEILVLYSRGG
                     FGYVSQNHMDMINYTQGFRFDKSDLIRNGDQPLADNKWYAAWIAQAPPGYWDPRKSEH
                     VEDLHQYYDDYMQSTYP"
BASE COUNT          511 a          510 c          467 g          472 t
ORIGIN      
        1 ttctactaca aactcggctt ccgaccggat acggcagccc tgcgacaaat ggccatcagc
       61 gagcagaaaa agatcgtttt gcggaaaggc tatcgctcct ctgaaaagac cctgctgaaa
      121 ttcacagaaa gcaatatctc cctgaagggc aagggacctg tacaacaggg agtctatgac
      181 atcacgtcca aagtgaagca gatgatcacc aggaaatatc agggcaacag tactgaagca
      241 gaagaagcat gcgtatccgc cttcctggct aagaccggaa gacaggatgc tttcacgaaa
      301 gaggaaaccg aagtgctgaa gcaggtctcc ctctgggcag ccgccatgaa catcacggat
      361 aaaaaacaac tgcatctatt gctcgaaacc gtgaagctga aaccggtgga tgaataccgc
      421 tatcaggcaa cactccgaca gttcttcggc gataagtatc aataggattt ctgatcactt
      481 catccggaag gtactccagt ccaccccgat gcggaaggtc tggtacagcc tgacgtcgct
      541 gatgcttccg ccaaaatggt tggtggcaga gtgcatcacc ccggaaggat atgccaccat
      601 acggttgaag cggtatccca cccggtcgat ctcctgccat ttgctgcgtt ttttggcatc
      661 gtcctcgaag agttgtcgca ggtcggacag tttcatgttg agatttcggg catcagcagc
      721 ggtcggacaa tcggtgatgc cggtcttttt atgcatccac agcgaagtgc cgcaatcgaa
      781 gggcaggtcg ggtgtcaggt agatcaccac ggtgatgtct tcgggcggat ggtcgtagtg
      841 cacgcccggc acttccttct tgtttccttt ggcgtagccg cagtagaaca ccccgttctc
      901 atccagcgga tcggtatcga agcgggtgat cttgattccc agtatccttt ccagttttct
      961 tttcacgccc ggctggtgat agaccgtacg actgcgcaga ccggtatagt accggggttc
     1021 gtagtaatcg gccgacatag cagcctgata cacctccatc ggatcatcat agaaatcatc
     1081 tttgaccatc acgaaccggg gatacaccaa cttgctgctc atatcgttgt ttggatacgt
     1141 tgataaaagt aagtgatcgt tgcttacata gtaatgacat ttatcaaacc agtatcctgt
     1201 ccgaatttgg tttcctgcat ctatcttcac gagatcaaac aatcatcccc atccccatga
     1261 gatccgccac cagtcatttt ctatcccgcc taagaacagg tatgtatggc ctgttgatct
     1321 tactgtctgt ttccctttcg gcacaaccca acgcccccat ctggcccaca gatgttcagt
     1381 ataagttcaa tatcgggacc tacacctgga ccgacagcaa cagctaccag ttcatggtgt
     1441 tgcaggtgac cggactacca gacatcaata cagaatacag caagatcatc gaactggagc
     1501 tttccttatc agaccaacca gctttcacct ggcagcagga tccgtattcc ggaggctatg
     1561 gctatgtttc cctcatcagc aaggatcaga cctttgcacc ggtcatggtc ctgaaggagg
     1621 tggtctatga cagtgtcagc ggcttccatc agatacaaca taccagtttg ttcctgacag
     1681 atgattttga ccggatcaaa caagacgaaa tactggtcct gtattcccgt ggcggctttg
     1741 gctatgtgtc acagaaccat atggatatga tcaattatac tcaaggattt cgctttgaca
     1801 aatcagatct gatcagaaat ggcgatcaac cactcgcaga taataaatgg tatgctgcat
     1861 ggatcgcaca ggcgcctccc ggatattggg atcccaggaa gtcagagcat gtggaagatc
     1921 tgcatcagta ttatgatgac tatatgcaaa gcacctatcc
//
LOCUS       DAOMAX010000409         1952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14607_length_1952_cov_3.425409, whole genome shotgun sequence.
ACCESSION   DAOMAX010000409 DAOMAX010000000
VERSION     DAOMAX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14607_length_1952_cov_3.425409"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PK742_13360"
     CDS             <1..312
                     /locus_tag="PK742_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HQU77627.1"
                     /translation="TLSDPRESLEQLISWGFQRILTSGGAGNAIDNLDTLKEYVQQAN
                     DRIIILPGGGVRPDNLAPIIGTTGAKEVHSSALHQPDPFNSFCDPAMIRLMKEQLSAT
                     A"
     gene            309..>1952
                     /locus_tag="PK742_13365"
     CDS             309..>1952
                     /locus_tag="PK742_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQU77628.1"
                     /translation="MTRTLLIWILLSTPLMIQAQHIQDLHEGWQFRESDGTEWLSAQV
                     PGSVHMDLYRNHVIPDPFYGTNEDSVQWIEERDWDYRLAFSADAETLNKEHIMLVFDG
                     LDTWAEVVLNGETILHAENMHLAYPVDVKRLLKEQNELLIHFISAVKKGKELAKDIPF
                     RMPSDERIYSRKAQYQFGWDWGPRFVGCGIWKDVRLEAWDDARILDLQVFQDSLSDEI
                     AEMHAILGWDFPILPIGHVEEFQVNLYVNDHLVNNQRVIASSEPTPCPDLYFSIDDPI
                     RWWPNVNLSNSSIGIPVDTSVTTLKTPYRYTVKAELLKGDSVISSAEQMIGLRTIELV
                     LEPDAQGTSFYFRVNGVPVFAKGANFIPTHSFPEQTTKAEYKAMLEEIKAANVNMLRI
                     WGGGIYEDDHFYDLCDSLGIMVWQDFMFACAMYPGDDDFLNNVSKEIDFQVRRLRNHP
                     SIVLWCGNNEIDEAWHNWGWQILYGYLQLEADKIWGWYTDLFHELIPQSLETLDPGRS
                     YWPSSPSIGWGRKESLTLGDMHYWGVWWGKEPFSVWKDKT"
BASE COUNT          504 a          521 c          517 g          410 t
ORIGIN      
        1 acgctgagtg atccacggga gagcctggag cagctcatca gctggggctt tcagagaatt
       61 ctcacttccg gcggtgcagg aaatgcgata gacaacctgg atacgttaaa ggagtatgtg
      121 caacaggcga acgaccgcat catcatcctg ccgggcggag gggtcagacc ggataacctg
      181 gcgcccatca tcggcaccac cggagcgaaa gaagtgcact cctcggctct ccaccagccg
      241 gatcccttca acagcttctg cgatcctgcc atgatacgcc ttatgaaaga acaactcagc
      301 gccactgcat gaccagaaca ttgctgatct ggatactcct cagcacaccg ctcatgatcc
      361 aggcacaaca catacaagac ctgcacgaag gctggcagtt tcgtgaaagc gacggcaccg
      421 aatggctttc tgctcaggta ccgggttcgg tgcatatgga cctgtaccgc aaccacgtca
      481 tccccgaccc tttctacggc acaaacgaag actctgtgca gtggattgaa gagcgtgact
      541 gggactatcg cctggcattt tctgctgatg cggaaacgct gaacaaagaa catatcatgc
      601 tggtattcga cggactggat acctgggcag aggtcgtgct gaacggcgag accatcctcc
      661 atgcagagaa catgcacctg gcttatccgg tggatgtaaa acggttgctg aaagaacaaa
      721 atgaactgct catacatttc atttcggccg tcaaaaaagg gaaggaactg gcgaaggata
      781 ttcccttccg catgccatca gatgaacgca tctacagcag gaaagcacag tatcagttcg
      841 ggtgggactg gggtccgagg ttcgttggtt gcggcatctg gaaggatgtc agactcgaag
      901 cctgggatga tgcccgcatc ctcgacctcc aggtatttca ggacagcctg agcgatgaga
      961 ttgcagagat gcatgccata ttaggctggg attttcccat tttacccata gggcatgtgg
     1021 aagagtttca ggtcaacctc tatgtgaatg accatcttgt caataaccaa agggtcatcg
     1081 ccagcagtga acccaccccc tgccctgacc tgtacttcag catagacgat cccatacgct
     1141 ggtggccaaa cgtgaatttg tcgaacagca gcattggcat ccccgttgac acctcggtca
     1201 ccacattgaa gacaccctac cgttatacgg taaaagcaga actacttaaa ggtgattcgg
     1261 tcatcagttc agcagagcaa atgatcggcc tgcgcaccat cgaactcgtg ctggaacccg
     1321 atgcacaggg aaccagtttt tatttcaggg tgaacggcgt gcctgtcttc gccaaaggcg
     1381 ccaacttcat ccccacccac agcttcccgg aacagaccac gaaagccgaa tacaaagcca
     1441 tgctggagga gatcaaagcc gccaacgtga acatgctgcg catctggggt ggaggcatct
     1501 atgaagacga ccatttctac gacctctgcg attcactggg catcatggtc tggcaggact
     1561 tcatgttcgc ctgtgctatg tatcctggcg atgatgattt cctgaacaac gtgtcaaaag
     1621 agatcgactt ccaggtgcgg cgactgcgca atcacccgag catcgtgctg tggtgcggca
     1681 acaacgagat agacgaagcc tggcataatt ggggatggca gatactctat ggttacctgc
     1741 aattagaggc cgataagatc tggggatggt acaccgacct ctttcacgaa cttataccac
     1801 agagccttga aaccctggat ccgggaaggt cctactggcc gagctctcct tccatcggct
     1861 ggggacgaaa agaaagcctc acactaggcg atatgcacta ctggggtgta tggtggggga
     1921 aggagccttt ttctgtctgg aaggataaga ca
//
LOCUS       DAOMAX010000410         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14666_length_1947_cov_3.743129, whole genome shotgun sequence.
ACCESSION   DAOMAX010000410 DAOMAX010000000
VERSION     DAOMAX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14666_length_1947_cov_3.743129"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="fabF"
                     /locus_tag="PK742_13370"
     CDS             complement(<1..1126)
                     /gene="fabF"
                     /locus_tag="PK742_13370"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQU77629.1"
                     /translation="MSTKRVVVTGLGALTPLGNSVQEYWAGLAAGRSGADFITRFDAE
                     KFKTRFACEVKNFDPTSVIDKKEVRRMDPYLHYAMAAVEEGVQDAGLTAEGIDHDKVG
                     VIWASGIGGLKTFEEEVSSFAQGDGTPRFNPFFIPKMISDIAAGMISIRYGFRGPNYA
                     TVSACASSTHALIAAFDAIRLGRAKVVVAGGSEAAVTASGVGGFNAMKALSERNDDPK
                     TASRPFDVDRDGFVLGEGAGCVIVEDYEHAVARGAKIYCELAGGGMSADAYHMTAPHP
                     DGIGATLVMRDALRDAGMQTTDIDYVNVHGTSTPLGDPQEIKAIEAVFGEHAYNMNIS
                     STKSMTGHLLGAAGAIEAIAGIMAMQHSLVPPTINHFTDDP"
     gene            complement(1148..1384)
                     /locus_tag="PK742_13375"
     CDS             complement(1148..1384)
                     /locus_tag="PK742_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQU77630.1"
                     /translation="MSEIAEKVKKIIVDKLGVDESEVVPEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAEGITTVGDAITYLEENVK"
     gene            complement(1510..>1947)
                     /locus_tag="PK742_13380"
     CDS             complement(1510..>1947)
                     /locus_tag="PK742_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQU77631.1"
                     /translation="DSKIHHNNLLNNILAKIEANQAGADDALMLDMHGFVAETNACNI
                     FLIKNGVLRTPYARACLPGITRKKVLALSPGAGLPAEEADLSVSEFYNADEVFTTGTM
                     GELVPVSSIDGRAISNRHAKKWLPILQEAYAEATASLGEKLDF"
BASE COUNT          453 a          511 c          512 g          471 t
ORIGIN      
        1 ccggatcatc cgtgaaatgg ttgatggtag gaggtaccag gctgtgttgc atggccatga
       61 tacctgcaat ggcttcgatg gcaccggcag cacccagcag gtgaccggtc atggatttgg
      121 tagagctgat gttcatattg taagcgtgct cgccaaacac tgcttcgatg gctttgatct
      181 cctgcggatc gcccagcggt gtagaggtac catgcacgtt cacatagtcg atatcggtgg
      241 tctgcatacc ggcatcacga agcgcatcac gcatgaccag agtggcaccg atcccatccg
      301 gatggggagc ggtcatatgg taagcgtcgg cactcatgcc tccaccagcc agttcgcagt
      361 agatcttcgc tccgcgggca acggcgtgtt cgtagtcttc cacaatgaca cagccggcac
      421 cttcgcccaa cacgaaaccg tcgcggtcca cgtcaaatgg acgggaagcg gtcttcgggt
      481 cgtcgttgcg ctcgctcagg gccttcatgg cgttgaatcc acccacaccg gaagcggtca
      541 ctgcagcttc agaaccaccg gccacaacaa ccttggcacg acccagacgg atcgcatcaa
      601 aagcggctat gagtgcatgg gtactggaag cacaggcaga taccgtggca tagttcgggc
      661 cgcggaaacc gtagcggatg gagatcattc cggcagcgat atcgctgatc atctttggaa
      721 tgaagaaagg attgaagcgg ggggtaccgt cgccctgggc gaacgaggat acttcctctt
      781 caaaagtttt gagacctccg ataccggaag cccagatcac acccactttg tcatgatcga
      841 taccttcggc ggtcagacct gcatcctgca ccccttcttc caccgctgcc atggcatagt
      901 ggagataagg atccatgcga cgaacctcct tcttatcaat gaccgaagtg ggatcgaagt
      961 ttttaacctc gcaggcgaac ctggtcttga acttctcagc atcgaaacgg gtgatgaaat
     1021 cagctccgct tcgaccagct gccaatccgg cccagtattc ctgcacagag ttgcccaggg
     1081 gcgtcaacgc acccagccct gttacgacaa ctcttttggt actcataaaa agaaaggtag
     1141 taggtggtta cttcacgttc tcttccaggt aggtgatagc gtcacctacg gtagtgatgc
     1201 cttcagcctg ttcgtccgga atggagatgt tgaactcctt ctcgaactcc atgatcagct
     1261 cgacagtgtc cagagagtca gcacccagat cgttggtgaa ggaagcttca ggtaccactt
     1321 cgctttcatc aacgcccagt ttgtcgacga tgatcttctt tactttttca gcaatttctg
     1381 acatgactaa ttgattaggt tttaattaaa gtgcaaagat aagagagggg gtggggtatt
     1441 tgcaaataac gcagcacgat agaacaggcc ttcagacgtt gataaattgt ataaacaggc
     1501 ctctgttgtt cagaaatcaa gtttttcacc cagactggct gttgcctctg cataggcttc
     1561 ctgtaagatt ggtaaccatt ttttagcatg cctgttgctg atggcccttc catcaatgga
     1621 agatacaggt accagctcac ccatggtgcc ggtggtaaat acttcatctg cattatagaa
     1681 ttcagacaca ctgaggtcag cttcttctgc aggcaggcct gcgcctggcg acaatgccag
     1741 cacttttttt cgggtgatgc cgggcaggca ggcacgggca taaggagtac gcaacacccc
     1801 gtttttgatc aggaagatat tgcaggcatt ggtctctgcc acaaaaccgt gcatgtctag
     1861 catgagtgcg tcatctgcac cggcctggtt ggcttctatc ttggccagta tattattgag
     1921 caggttgttg tggtggatct tgctgtc
//
LOCUS       DAOMAX010000411         1929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_14925_length_1929_cov_4.688367, whole genome shotgun sequence.
ACCESSION   DAOMAX010000411 DAOMAX010000000
VERSION     DAOMAX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14925_length_1929_cov_4.688367"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="pyk"
                     /locus_tag="PK742_13385"
     CDS             complement(<1..945)
                     /gene="pyk"
                     /locus_tag="PK742_13385"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQU77632.1"
                     /translation="MTRHDHRTKIIATLGPASGNEEMIRKLVQSGVDVFRLNMSHGDH
                     AYHKKTIKLIRKINKDLGTYVAILADLQGPKIRLGMVEENLPPLKKNAELIITTKELL
                     GNNEQVYITYPAFAKDVEPGDTILIDDGKIEGRVLTSNKKDKVTVRIIHPGVLSSRKG
                     VNLPDTNVSIPSLTEKDHEDLLFALENGAHWVALSFVRKAEDIIHLKNIIRKHGKLTK
                     VIAKIEKPEGFRNIDAIIDATDAVMVARGDLGVEMPVENVPFIQKSIVTKCGKKAKPV
                     IIATQMMESMIEQARPTRAEVTDVANAVMDGADAVMLSG"
     gene            complement(947..1360)
                     /locus_tag="PK742_13390"
     CDS             complement(947..1360)
                     /locus_tag="PK742_13390"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HQU77633.1"
                     /translation="MKKTQLRFERDTDFLLQGLVCAIPDYRLGWQLNRHLGIRLVRQD
                     DIAPPGETGQPQFSCFLCEEPITHSAFYLIQNKYNGTHYAPEWKKIDYFLMIKGSWYL
                     DRREDIATALKQLPDVQAVIGIEPKTIRNKDLFQL"
     gene            1432..>1929
                     /locus_tag="PK742_13395"
     CDS             1432..>1929
                     /locus_tag="PK742_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQU77634.1"
                     /translation="MASGKDKNKPSRLTGSSDTALLRRLLGYAGPYRKQFILSALLAI
                     LLAILGPLRPYLVQLTVDRYIFPKDLHGLIIISLISLAVLVVESYLRYQFLYITNWLG
                     QHVVRNLRMRVFNHTIRLRLSYFDKTPVGTSTTRTINDLETINSVFTEGIIQIIADLL
                     TIIFVI"
BASE COUNT          395 a          502 c          489 g          543 t
ORIGIN      
        1 accactcagc atgacggcat cggcaccatc catgaccgca ttggccacat ctgtcacttc
       61 tgccctggtc ggtctggctt gttcaatcat actctccatc atctgggtgg cgatgatgac
      121 gggcttggcc ttcttaccgc atttggtgac gatggatttc tgtatgaaag gcacgttctc
      181 taccggcatt tccacgccga gatctccgcg ggccaccatg acggcatcgg tagcgtcgat
      241 gatggcatcg atgttccgga agccttccgg tttttcgatc ttggcgatga ccttggtgag
      301 tttgccgtgc ttgcggatga tgttcttcag gtggatgatg tcttccgcct ttctcacaaa
      361 tgacaaggcc acccagtggg caccattctc cagtgcaaag agcaggtcct catggtcctt
      421 ttctgtcaga ctgggaatgg acacattggt atcaggcagg ttcactcctt tcctgctgct
      481 cagcacaccc gggtggatga tgcgcacggt caccttatct tttttgttgg aggtaagtac
      541 cctgccttct attttgccat cgtctatcag gatggtatcg ccgggctcca catcttttgc
      601 aaaggcggga taggtgatat acacctgttc attattaccg agcagttctt tggtggtgat
      661 gatgagttcg gcattctttt tcagcggcgg aagattttcc tccaccatcc ccaaccggat
      721 cttgggtccc tgcagatcgg ccaggatggc aacgtatgtg ccaaggtcct tgttgatctt
      781 ccggatcagt tttatcgtct tcttgtggta ggcatgatcg ccgtgcgaca tattcaggcg
      841 gaacacatcc accccgcttt gtacgagctt gcggatcatc tcctcattgc cagatgcggg
      901 tcccagggtg gcaatgatct tggttctgtg atcgtgtcgg gtcatattac aattgaaaca
      961 ggtccttatt cctgattgtt ttgggttcga tgccgatgac cgcctgcaca tccggtaatt
     1021 gtttcagtgc ggtggcgatg tcttcacgcc tgtccaggta ccagctgcct ttgatcatga
     1081 ggaagtagtc tatcttcttc cattcaggag catagtgggt accgttgtat ttgttctgga
     1141 tcaggtaaaa ggccgaatgc gtgatgggct cttcgcagag gaaacaactg aactgcggct
     1201 gtccggtctc tccgggagga gcaatgtcat cctgcctgac cagccggatg cccaggtgcc
     1261 ggttcagttg ccagcccagg cgatagtcgg ggatggcgca gacaagtccc tgcagcagaa
     1321 agtctgtatc acgttcgaac cgcagttgtg ttttcttcat cggtcgatgg taaaattacg
     1381 atgaaagcag agaaaggggt ccgctgtttt atccatattt ttaaagccgt tttggcttcc
     1441 ggaaaagaca agaacaaacc atcccgactt acgggcagct cagatactgc tttattgcgc
     1501 aggttgctgg gatatgccgg tccttacaga aagcaattta tcctctctgc acttttagcc
     1561 attttactgg ccatactggg tccgctccga ccttacctgg tacaacttac tgtagatcgg
     1621 tacatctttc ccaaagatct gcatggcctc atcatcattt cacttatttc actggcagtg
     1681 ctggtagtgg aatcatattt gcgataccag ttcctgtaca tcaccaactg gctggggcag
     1741 catgtggtca ggaatctgcg gatgcgggtg ttcaaccaca ccatccgttt gcggctgtcc
     1801 tattttgata aaacaccggt aggtacttcc actacccgga ccatcaatga cctggagacc
     1861 atcaactcgg tgttcacgga aggcatcatc cagatcatcg ccgatctgct caccatcatc
     1921 tttgtgatc
//
LOCUS       DAOMAX010000412         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15029_length_1921_cov_4.614684, whole genome shotgun sequence.
ACCESSION   DAOMAX010000412 DAOMAX010000000
VERSION     DAOMAX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15029_length_1921_cov_4.614684"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..843
                     /locus_tag="PK742_13400"
     CDS             154..843
                     /locus_tag="PK742_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77635.1"
                     /translation="MTEKTKSVILALVTGSSGSSAQFAGKKSLGWGLLLFCWVVLPAV
                     IWLIRTNTTLNPQMPVILFYSGIILVHLALALYHGIGREASQSVDAVRSRTGRIALSV
                     LIMFVLAYISHHYVFTDRPVDTSKAKPVVSIASVTLSQEFLEDEKAFRKQYDRQVIKV
                     TGKVVSIGEDFSEGSYIALEGYPGSPTDVNCYFLPERQAEFTIIASGQEVSVTGVVDG
                     RFLRNCYLNKP"
     gene            852..1475
                     /gene="recR"
                     /locus_tag="PK742_13405"
     CDS             852..1475
                     /gene="recR"
                     /locus_tag="PK742_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQU77636.1"
                     /translation="MKLPSRIMEDAVTEFSRLPGIGKKTALRLVLHLMQEDASRVGQM
                     AESLQRLKTELRYCKRCFNIAEDDLCSICMGRNRQHQMICVVESLRDLLAIENTQQYQ
                     GVYHILGGVISPVEGVGPEQLHITELIDRLPGLESPELILALNPDIEGDTTAFYIHAQ
                     IKKAGLDVRITSIARGISFGGELEYTDEITLARSLAARMPYQRNSDS"
     gene            1478..>1921
                     /locus_tag="PK742_13410"
     CDS             1478..>1921
                     /locus_tag="PK742_13410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQU77637.1"
                     /translation="MDLSIIIVNYNVRHFAELTIESALEACKDLDAEIILVDNNSTDG
                     SLPWLSEQFPSVRVIANQENTGFAVANNQGIREASGRYILLLNPDTVVAEDTFKKCLA
                     YLDTHPDTGGLGVRMLDGSGRFLPESKRKFPGPSVAFFKAFGLSAL"
BASE COUNT          527 a          440 c          464 g          490 t
ORIGIN      
        1 cagcggcaac aatagaacaa tatacagtgc ccatctcttc atactttcag aaacctctga
       61 cttggcaata tttcaaaagt aaggatattc attagccgaa gcaacgcaat aaggatgcat
      121 acttagtaat tttgcagaaa ttgtccacat tttatgacag aaaagacaaa atcggtaatt
      181 cttgcccttg tcacaggaag cagcggaagc agcgctcaat ttgcaggaaa gaaatctctt
      241 ggatgggggc tgttgctctt ctgctgggtg gtgctgcctg ctgtcatctg gttgatcaga
      301 accaatacaa cactcaatcc gcaaatgccg gtcatcctgt tttattcggg tatcatactc
      361 gtgcacctgg cacttgcact gtatcatgga attggtcggg aagcatcgca atctgtagat
      421 gcagttcgct cacgaacagg cagaatagct ttgtcagtgc tgatcatgtt tgtcctggcc
      481 tacatcagcc atcactatgt attcactgac cggccggtag ataccagcaa ggccaaacct
      541 gtggtcagta ttgcctcagt tactttgagt caggaatttc tggaagatga aaaagctttc
      601 agaaaacaat atgacaggca ggtcatcaag gttacaggaa aggtcgtttc catcggagaa
      661 gatttttctg aaggcagcta catagcgctg gaaggatacc ctggcagtcc aacagatgtg
      721 aattgctatt ttctgccaga aagacaggca gagttcacta ttattgccag cggacaggag
      781 gtgagcgtaa ccggtgtggt tgatgggcgc tttctgagaa actgttacct caacaaaccc
      841 tgaacaacca gatgaaactg ccatcccgga tcatggaaga cgctgtaaca gaattcagca
      901 ggcttcctgg tatcggaaag aaaacagctc ttcggctggt tttacatctg atgcaggaag
      961 atgcttccag ggtcggtcag atggctgagt cactgcaacg cttaaaaaca gaactgcgct
     1021 attgcaaacg ttgcttcaat attgctgaag atgatctttg ttccatatgc atgggaagaa
     1081 accgccagca ccagatgatc tgtgtagtag aatcacttcg cgatctgctg gcaatagaaa
     1141 acacccagca gtaccagggg gtttaccata tattgggcgg tgtcatctcg cctgttgaag
     1201 gagtcgggcc tgaacaactt catatcactg agctcattga tcgacttccg gggttggaat
     1261 ctcctgaact tattctggcc ctgaatccgg atatagaagg cgataccaca gccttctata
     1321 tacatgcaca gatcaaaaaa gccggattgg atgtcaggat cacttctata gcaagaggta
     1381 tttcatttgg aggcgaactg gaatacactg atgagataac cctggcaaga tctctcgctg
     1441 cccgaatgcc gtatcaacga aactccgaca gctgaaagtg gatctcagta tcatcatagt
     1501 taattacaat gtgcggcatt ttgctgagct gaccattgaa tcagcgctgg aggcctgtaa
     1561 agatctggat gcagagatca ttctggtaga taacaactcc accgacggca gccttccctg
     1621 gctcagcgaa caatttccat ctgttcgggt gatcgccaat caggagaata cgggctttgc
     1681 tgtagccaat aatcaaggca tccgtgaagc ttccggcagg tatattttgc tgcttaatcc
     1741 ggacacggta gtggcagaag acactttcaa aaaatgtctg gcatatctgg atacccaccc
     1801 tgacacagga ggcttaggtg tgcgcatgct ggatggaagc ggcagatttc ttcctgaatc
     1861 caagcgtaag ttcccgggtc cttcagttgc cttcttcaag gcgtttggct tatctgccct
     1921 t
//
LOCUS       DAOMAX010000413         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15138_length_1914_cov_3.995697, whole genome shotgun sequence.
ACCESSION   DAOMAX010000413 DAOMAX010000000
VERSION     DAOMAX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15138_length_1914_cov_3.995697"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK742_13415"
     CDS             <1..643
                     /locus_tag="PK742_13415"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQU77638.1"
                     /translation="GAILSVVILYWKRFFKTFGFYWKLLVAFIPAVIAGLLFGDKIDA
                     LLENVTVVAISLVAGGIILLFIDRLFSDDKATDATEPTYKSSFIIGLFQCLAMVPGVS
                     RSAATIIGGLTQKLTRKAAAEFSFFLAVPTMLAATVYKLYQFYGDGYSFGSHEWTPLI
                     LGNVVAFIIAIFAMRSFVQFLTRNGFRLFGWYRIIIGSAILLMLALGVDLQIV"
     gene            640..1356
                     /gene="truB"
                     /locus_tag="PK742_13420"
     CDS             640..1356
                     /gene="truB"
                     /locus_tag="PK742_13420"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQU77639.1"
                     /translation="MISSPQTYTEGTFILFDKPLGWTSHDVVNRVRYHLTRYCGVKKL
                     KVGHGGTLDPKATGLLVVATGPNTKKLQYLIDADKEYTGTIKLGAVTASYDGETAEEQ
                     RKPWEHITREDVLRVLEKFTGVQQQVPPIHSAIKQDGRPVYLAARAGETPEMKSRSIN
                     ISELELTDFEPPYVHLRVACSKGTYIRSLAHDIGQELGCGAWLSGLRRTRIGSFLAEN
                     ALDTEAFIATLQELRNKPKS"
     gene            1368..>1914
                     /locus_tag="PK742_13425"
     CDS             1368..>1914
                     /locus_tag="PK742_13425"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HQU77640.1"
                     /translation="MQVHRSLDHLPNFRNAVITIGTFDGVHHGHQAIIKSIVEDARSL
                     QGESIIITFHPHPRNIIRPEQTLFCLSTIQEKLELLEKLGVDHVVVVPFSREFSEMSA
                     AGYVEDFLLAKFHPKRIVIGYDHKFGRDRSGDIHLLRQLAEAAGVEVAEIGQQQIEDI
                     TVSSTRIRKYLQEGDVATAATL"
BASE COUNT          458 a          503 c          501 g          452 t
ORIGIN      
        1 gggtgctatt ctctctgtgg tcatcctgta ctggaaaagg tttttcaaaa ccttcgggtt
       61 ctactggaaa ttgctggtgg cattcatccc tgcggtcatt gcgggattgc tgttcggcga
      121 taagatagat gccctcctgg aaaatgtgac cgtagtggcc atctcgctgg tggcaggtgg
      181 tatcatcctg ctgttcatcg atcggctgtt cagcgatgac aaagctacag atgccaccga
      241 gccaacttat aagagttcct tcatcatagg tctcttccag tgcctggcca tggtgccggg
      301 tgtatcccgt tctgctgcta ccatcattgg ggggcttacc caaaaactga ccagaaaagc
      361 agctgcagaa ttcagtttct tcctggccgt acccaccatg ctggctgcca ccgtctataa
      421 actatatcag ttctatggag atggctacag tttcggcagc catgaatgga cacctctgat
      481 actgggtaat gtggtggcct ttattatcgc catctttgcc atgcgctctt tcgttcaatt
      541 tctcaccaga aacggattcc gcctctttgg ctggtaccgc atcatcatag gctctgcgat
      601 cctgctcatg ctggccctgg gtgtggacct gcagatcgta tgatttcatc tccgcagaca
      661 tacacagaag gtactttcat cctgttcgac aagccgttag gctggaccag tcacgatgtg
      721 gtgaaccggg tgcgttatca tttgacacgc tactgcggag tgaagaagct gaaggtgggg
      781 cacggaggga cactggatcc caaagccacc ggattgctcg tggtggccac gggtccgaac
      841 acgaagaaac tgcaatacct gatcgacgcc gacaaggaat ataccggtac catcaaactg
      901 ggagcggtca ctgcctccta cgatggtgaa acagcagagg aacaacgcaa accctgggag
      961 catatcaccc gggaagatgt gctgcgtgtg ctggaaaaat tcaccggtgt gcagcaacag
     1021 gttccgccca ttcattctgc catcaaacag gacggaagac cggtctatct ggctgcccgt
     1081 gcaggtgaaa cgcctgaaat gaagtcccgc agcattaaca tcagcgaact ggaactcact
     1141 gactttgaac ctccgtatgt acaccttcgt gtggcatgca gcaagggcac ttacatccgt
     1201 tctcttgccc atgacatcgg acaggaactg ggatgtggtg cctggttatc cggcctgcgc
     1261 agaacacgta tcggttcctt cctggcagaa aatgcactgg atacggaagc attcatagcc
     1321 accttgcagg aactgagaaa caagccgaag tcctaatttt gcccgacatg caagtacacc
     1381 gcagcctgga tcatctgcca aattttcgga atgccgtcat caccatcggc actttcgacg
     1441 gtgtgcatca cggacaccag gccatcatca aaagcattgt ggaggatgcc cgttccctgc
     1501 agggtgagag catcatcatc acctttcatc cgcatccccg caacatcatc cggccggaac
     1561 agaccctgtt ctgcctgagc accatccagg aaaaactgga gctgctggaa aagcttggcg
     1621 tggatcatgt ggtggtggta ccgttcagcc gtgagttcag tgagatgagt gcagcagggt
     1681 atgtggaaga ttttctgctt gctaaattcc atccgaaacg catcgtgatc gggtatgatc
     1741 ataaattcgg tcgtgacaga agtggtgata tccacttact gcgtcaactg gcggaggctg
     1801 ccggtgtgga agtggcagag ataggtcagc aacagatcga agacatcacc gtttccagca
     1861 cccgcatacg gaaatattta caggaaggag atgtagctac cgccgctacc ttac
//
LOCUS       DAOMAX010000414         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15256_length_1904_cov_3.713359, whole genome shotgun sequence.
ACCESSION   DAOMAX010000414 DAOMAX010000000
VERSION     DAOMAX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15256_length_1904_cov_3.713359"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..471)
                     /locus_tag="PK742_13430"
     CDS             complement(16..471)
                     /locus_tag="PK742_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77641.1"
                     /translation="MYLHEEGKNDPLSSEPLQQKAMEILRLVRALHDAAQADDTDLTE
                     DTELMMEREALDILMGNALLIPGKIRANIQVEAYWLRMENAVLIKRAAMEIQSMVHQL
                     LFRHPDWQDYADMIIEEVEQFRGLFINWVGRFDSSIDPDDGWGLFDRRT"
     gene            complement(572..>1904)
                     /locus_tag="PK742_13435"
     CDS             complement(572..>1904)
                     /locus_tag="PK742_13435"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77642.1"
                     /translation="IVNSGGEAIAGSGGTWCVNFDASGTTGYALATGTLTSSPQKSVQ
                     PQILKTTDGGGTWSWLPGYDWSTNTTMQEWVVAQDGGTGDIVPYFTEIDGTVGSDGRL
                     HMFAEVLSSFSGNYLDSMYFIYADYRVYMHLSTSDGADWEVETLGYSLLPEGEFGSTP
                     VSVTQNPQISRTPDGSKLFFAWNESDSLIFTDHSAPDLFVLGYDIASGEYTNPVNITD
                     GTDYTYSAYYPTMAPVSMDNGAGEYELPIVFSVPGADDVSPPQFYYAKGIIFTDDDFG
                     AEPAAIANFTFSVSGATATFTNSSSNATSYSWNFGDGSGTSPLESPVYTYTASGTYNV
                     CLTASNATSSDETCKNVTATVVGIEDLLLDAALNIYPTPANEMMNINLNGNFSNVSVE
                     IFNLLGESVMSPISLNGGSATVNVAGLAEGNYIVKLTSDNGYAVRQITIAR"
BASE COUNT          504 a          464 c          463 g          473 t
ORIGIN      
        1 taaggaggcg gtcactcagg ttctacggtc aaataatccc cagccatcgt ccggatcgat
       61 cgagctatcg aaccggccta cccagttaat aaaaagacca cgaaactgtt ccacttcttc
      121 gatgatcata tctgcatagt cctgccagtc ggggtgtctg aacagcagct ggtgcaccat
      181 gctctggatc tccatagcgg ctctcttgat cagtactgca ttctccatcc tcagccagta
      241 agcttcaacc tggatattag ctctgatctt tccagggatc aacagggcgt ttcccatcaa
      301 tatatccagc gcttcccgct ccatcatgag ttcggtgtct tccgtcaggt cagtatcatc
      361 agcctgagca gcatcatgaa gtgcccttac caggcgaagt atctccatgg ccttctgctg
      421 caatggttca gatgatagcg ggtcattttt tccttcttca tggagataca tagggaaagg
      481 gttattgctg caaagtaaga agatgcgggc acataaggca caaaaaaacc cggacgaaac
      541 cgtccgggtt ttttattggg tctaagaccg attatctcgc gatagtgatc tgacgtacgg
      601 cataaccgtt atcagaggtc agcttcacaa tgtagttacc ttctgcaagg ccagctacat
      661 taacagtggc agaaccacca ttcaggctga tcgggctcat tactgactca cccagcaggt
      721 tgaagatctc aacgcttaca ttgctgaagt taccattgag gttgatgttc atcatctcat
      781 tggcaggagt cgggtagata ttcagagcgg catccagcag gaggtcttcg ataccaacta
      841 cagttgcagt aacattctta caggtctcat cgctggaagt tgcgtttgat gctgtcagac
      901 acacattata ggtacctgaa gcagtataag tataaaccgg gctttccagc gggctggtac
      961 ctgaaccatc accgaagttc cagctatagc tggtagcatt ggaagaagag ttggtgaatg
     1021 tggcagttgc cccagataca gagaaagtaa agttagctat tgcagcaggt tcagcaccaa
     1081 agtcatcatc agtgaaaatg atgcctttag cgtaatagaa ctgaggcgga gaaacatcat
     1141 cagcaccagg aacgctgaac actataggca gttcatactc accggcacca ttgtccatag
     1201 atacaggagc catggtagga tagtaagctg aataggtata atcagtaccg tctgtgatat
     1261 ttaccgggtt ggtgtattca ccggaagcaa tatcataacc cagtacgaac aggtcaggag
     1321 cactgtgatc agtaaagatc agagagtcag actcgttcca ggcaaaaaac agcttggatc
     1381 catcaggtgt gcggctgatc tgcgggttct gtgttactga aacaggagtg ctgccaaatt
     1441 caccttcggg taacaggctg taacccaggg tttccacttc ccagtcagct ccatctgaag
     1501 ttgacaggtg catgtacact ctgtaatctg catagatgaa gtacatggaa tcgaggtagt
     1561 tgccggagaa tgagctcagc acttctgcaa acatatgcag tctgccatct gaaccaacgg
     1621 tgccgtcaat ttcagtgaaa taaggaacga tatcaccagt tcctccatct tgagctacta
     1681 cccattcttg catcgtggtg ttggttgacc agtcatagcc tggcagccag ctccatgttc
     1741 cgccaccgtc ggtggtcttc aagatctgag gctgaactga tttctgtggg ctgctggtaa
     1801 gcgtaccggt tgcaagagca tagccggtag ttccggaggc gtcaaagtta acacaccagg
     1861 ttccgccgga accggctata gcttcaccac cagagttgac gatc
//
LOCUS       DAOMAX010000415         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15468_length_1890_cov_3.232153, whole genome shotgun sequence.
ACCESSION   DAOMAX010000415 DAOMAX010000000
VERSION     DAOMAX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15468_length_1890_cov_3.232153"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK742_13440"
     CDS             <1..227
                     /locus_tag="PK742_13440"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQU77643.1"
                     /translation="ILFTMPAHRADAVNGHIYFFRPADADLDRTMDLAPDASGQQIIP
                     VSNLVPGRYTVKISWNRQEISYYWEKEITL"
     gene            227..937
                     /locus_tag="PK742_13445"
     CDS             227..937
                     /locus_tag="PK742_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQU77644.1"
                     /translation="MEWILAAAVIGLMGSLHCMGMCGPIAFMLHSRVPGSAWAHTVLY
                     NTGRITTYALLGAIAAAIGQRIAMAGLQQVLSIVSGLLILLILLLNAGRKVKWVNVIY
                     EPVATFIRRSMSRILPKPTVPGFYLAGMVNGLLPCGLVYVALAAALNTPGIAEGAVFM
                     AVFGLGTFPAMIFAGMAGRFMNLQRRLKLRRLLPWFTAAVAVLLIVRGLDLGIPYVSP
                     HTSTQGQVMDCCSQPMVH"
     gene            complement(938..>1890)
                     /locus_tag="PK742_13450"
     CDS             complement(938..>1890)
                     /locus_tag="PK742_13450"
                     /inference="COORDINATES: protein motif:HMM:NF023380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HQU77645.1"
                     /translation="GAIISSVEDLLLWDEALYSDKLVSQKTLQQAFEPHLLADGTNTN
                     YGFGWGVSVYKELTFIRHGGAINGFLSDAVRIPEAHFFMVILSNNQSVGPAGLTDEIL
                     NKAFSLGIANTTEQSCEDYAEELTGVYQVVRSGGRLVTNYSEEPVYGYVRYADGALTI
                     QQTGGGTRTLTCIGKDTFMLRSPYTRMVFTRDENDRVLGMHLLTLPTTFGPDDFAPKT
                     DIPLPGEKQEVSLLPEQLIRLCGSYDLGGGFLLSVTAEGSQLFIQATGQAKFPVYPTS
                     DTEFFWKIVDASVTFETNEDGSISGITLHQGGDYFGRKIN"
BASE COUNT          438 a          486 c          468 g          498 t
ORIGIN      
        1 atattttgtt taccatgccc gctcaccggg ctgatgcggt gaacggtcat atatacttct
       61 tccgcccggc agatgcagat cttgatcgca ccatggatct tgctccggac gcatcgggtc
      121 agcagatcat accggtaagc aacctggtgc ccggccggta cacagtgaag atcagctgga
      181 acagacagga aatatcttat tactgggaaa aagaaatcac cctctgatgg aatggatcct
      241 ggcagcagct gtcataggcc tgatgggtag tttgcactgc atgggtatgt gcggtcccat
      301 tgccttcatg ctgcactcca gagtacccgg atcagcctgg gctcacacgg ttttgtacaa
      361 taccggaagg atcactacct acgccttgct gggagccatt gctgcggcca ttggtcaacg
      421 cattgccatg gcaggtttgc aacaggtttt atctattgta agtggtctgc ttattctgct
      481 cattctgttg ctgaatgccg gcagaaaagt caaatgggtg aacgtgatct atgagcctgt
      541 agcaacattt atcagaagat ccatgtccag gatcctgccg aaaccaactg tgcccggttt
      601 ttacctggcc ggaatggtga acggtctatt gccctgcgga ctggtatatg tggcattggc
      661 agcggcactc aatacaccgg gaatagccga aggtgcagta ttcatggctg tatttggtct
      721 gggcaccttt cctgctatga tctttgccgg tatggcgggc agattcatga acctgcaacg
      781 cagactaaag ctgcgccgtt tgctgccctg gttcacagcg gctgtggctg tgctgctgat
      841 cgttcgcggg ctcgatcttg gcattccata tgtaagcccg cacaccagca cccagggtca
      901 ggtgatggac tgttgcagcc agcccatggt gcactgatca gttgatcttc ctgccaaagt
      961 aatcgccccc ctgatgcagg gtaatgccac tgatgctgcc gtcttcattg gtctcgaaag
     1021 tgaccgaggc gtccacgatc ttccagaaga actcagtatc ggaagttgga tacacaggaa
     1081 attttgcctg tcctgttgcc tgaatgaaaa gctgtgaacc ttcggctgta actgatagca
     1141 gaaatccacc tcccagatca taagatccgc aaagtctgat cagttgctca ggcagcagac
     1201 ttacctcctg tttctccccc ggcaagggta tatccgtttt cggcgcaaaa tcatccgggc
     1261 cgaaagtggt aggaagtgtc agcagatgca ttcccagaac ccggtcgttc tcgtctctgg
     1321 taaataccat acgggtatat ggtgagcgca gcataaatgt atcttttcca atacaggtaa
     1381 gggttcttgt tccaccaccg gtttgctgaa tggttagcgc accgtcagca tatctgacat
     1441 acccgtacac cggttcctca ctgtaattgg ttaccagtcg tcctcccgaa cgaactacct
     1501 gatatacacc tgtcagttct tcagcatagt cttcgcatga ctgttcggta gtgttggcaa
     1561 taccaagact gaatgccttg ttcagaatct cgtctgtcaa gcctgccggc ccaacagatt
     1621 gattgttact tagtatgacc atgaagaaat gtgcttccgg aatgcgcact gcatcactca
     1681 gaaatccatt gatagcacct ccgtgtctga taaaggttag ttccttatag actgatactc
     1741 cccatccaaa tccgtagttg gtattggttc catctgccag cagatggggt tcaaatgcct
     1801 gttgcagggt cttttgtgat accagtttat cactatataa tgcttcatcc cacaatagca
     1861 gatcttccac tgaagagatg atggccccgg
//
LOCUS       DAOMAX010000416         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15553_length_1885_cov_4.854645, whole genome shotgun sequence.
ACCESSION   DAOMAX010000416 DAOMAX010000000
VERSION     DAOMAX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15553_length_1885_cov_4.854645"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1668
                     /gene="guaB"
                     /locus_tag="PK742_13455"
     CDS             199..1668
                     /gene="guaB"
                     /locus_tag="PK742_13455"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQU77646.1"
                     /translation="MENLSAKFLGTSLTFDDLLVVPAYSEVLPREVDISTRLTRNLRI
                     NVPIVSAAMDTVTEFRMAIAMAREGGIGIIHKNMPIAAQAEQVRRVKRADSGMIMDPV
                     TLNQHALVGEAFQLMKENRIGGIPVVDDEGHLVGILTNRDLRFETNTERPIAELMTKE
                     NLITAPIGTTLQQAEEILRNHRIEKLPVVDDKNFLKGLITYKDILKLQSHPRACKDQY
                     GRLLVGAAVGVTADTLERVAALKNVGADMVIIDTAHGHSKGVLDMVKQVKKQFTDLEV
                     VAGNIATGSGAKALVDAGADGVKVGVGPGSICTTRVVAGVGVAQVSAIHEASKAIAGS
                     GVPVIGDGGIRYTGDIVKAIVAGADTIMAGSLLAGTEESPGETIIYEGRKFKSYRGMG
                     SVEAMEKGSKDRYFQDVEDDIKKLVPEGIVGRVPFKGTVAEVMVQYIGGLRAGMGYAG
                     AHDIEALKQAQFVQITSSGIRESHPHDVVITKEAPNYSR"
BASE COUNT          503 a          425 c          535 g          422 t
ORIGIN      
        1 tcctgctcac acaagcactc caatccggct cggccaatcg cctgccggtg gcagaactac
       61 ctgtgggtac ctatgtggca gtaatacgca atgaaaccgg agaagtaaaa ggtaagcagg
      121 tatgggtgaa acagtgaccg tctgtttgga tttcagtgtg ggaaatgaat tatttaattc
      181 ccctttctat ctttgcacat ggaaaatcta tctgccaagt tcctcggcac cagcctcacc
      241 ttcgatgatc tgttagttgt tcccgcttat tcggaagtgc ttccgagaga agtggacatc
      301 tccacccgtc tgacccgcaa ccttcgcatc aacgtaccca ttgtgtctgc tgcgatggat
      361 accgtaaccg aattccgtat ggccattgca atggcaagag agggcggtat cggcatcata
      421 cataagaaca tgcccatcgc cgcacaggca gaacaggtgc gcagggtgaa gcgtgctgac
      481 agtggtatga tcatggatcc tgttacacta aatcaacatg cattggtggg tgaagctttc
      541 cagctgatga aagagaaccg catcggcggc attccggtgg tggatgatga aggccacctg
      601 gtgggcattc tgaccaatcg cgacctgcgt ttcgaaacca acaccgaacg cccgatcgct
      661 gagttgatga cgaaggagaa tctgatcacg gcacctatcg gcaccaccct gcaacaggca
      721 gaagagatcc tgcgcaatca ccgcattgaa aaactgccgg tggtggacga caagaatttt
      781 ctcaagggac tgatcaccta taaagacata ttaaaactgc aaagtcatcc gcgggcctgt
      841 aaagaccagt atggccggtt actggttggg gctgctgtgg gcgtaacggc agacacactg
      901 gaaagggtgg ccgcattgaa aaatgttggt gccgacatgg tcatcatcga caccgctcac
      961 ggtcactcca aaggtgtcct ggacatggtg aagcaggtaa agaaacaatt cacagatctg
     1021 gaagtggtgg caggtaacat cgcaactggc agcggcgcca aagccctggt agatgctggg
     1081 gcagatggtg tgaaagtagg tgtcggtccg ggttccattt gtaccaccag ggtcgtggcc
     1141 ggtgtgggcg ttgcccaggt gagtgcgatt cacgaagcat ccaaagccat tgctggcagc
     1201 ggggttcccg tgataggcga tggcggtatc cgctacaccg gagatattgt taaagcaatt
     1261 gttgccggag ccgataccat catggcagga agtctgctgg caggtacgga agaaagtccg
     1321 ggcgagacca ttatttacga aggccggaaa ttcaagagct accgcggtat gggctccgta
     1381 gaggcgatgg aaaaaggcag taaggaccgg tacttccagg atgtggaaga tgatatcaag
     1441 aagctggtgc cggaagggat cgtgggaaga gtacccttca agggaacagt agctgaagtg
     1501 atggtgcagt atatcggcgg attacgtgcc ggaatgggtt atgccggtgc acatgatatt
     1561 gaagcattaa agcaggcgca gttcgtgcag atcacttctt caggaattcg tgagagccac
     1621 ccgcatgatg tggtcatcac caaagaagcg ccaaattaca gcaggtgaga ggagaatttg
     1681 gaaatgaggt aatgtggaaa tttgagaatg agtaaatggt tgtttttatc gcaatagttt
     1741 taccatagaa tggcaagaac aataccttat accataactt tataaggtga gaaccgcggt
     1801 tttcctactg ctttgtatgc atgccctagt cgtatttggg caagactggt atccctggaa
     1861 gccggataca gaatactatt attca
//
LOCUS       DAOMAX010000417         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15559_length_1885_cov_3.305464, whole genome shotgun sequence.
ACCESSION   DAOMAX010000417 DAOMAX010000000
VERSION     DAOMAX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15559_length_1885_cov_3.305464"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..825)
                     /locus_tag="PK742_13460"
     CDS             complement(121..825)
                     /locus_tag="PK742_13460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQU77647.1"
                     /translation="MSRFAISDIHGCAKTFEALLEQIALTKDDTLYLLGDLIDRGPGS
                     KEVVDKVLQLQADGYKVECLLGNHEWLLLNAPYDDSRLINWLSRNGGAITLQSYGIQE
                     DDFLSALPTDHAKFYRNLQYIIELEDYILVHAGLNFRDEDPLSDYTAMLWIRDWQKSM
                     DTQVTGNRKIVHGHTPRALHYLEADVKNEKCMVFNIDCGCVYSGDHFKGHLVALNLDT
                     LEVFSQENLEGLVLVP"
     gene            complement(827..>1885)
                     /locus_tag="PK742_13465"
     CDS             complement(827..>1885)
                     /locus_tag="PK742_13465"
                     /inference="COORDINATES: protein motif:HMM:NF036482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77648.1"
                     /translation="GRPYWPSSPSIGWGRKESLTQGDMHYWGVWWGKEPFSVWKDKTG
                     HFMSEYGFQGMPSWFTLQETMDSSQLKLLSPSMKTHQKHPVGYETIDEYMQRDFPVPD
                     NLEDYWYVSQLLQAWGIQQAFDAHLSAMPVCMGTLFWQWNDCWPVTSWSAVDHAGRRK
                     ALYYAARRTFGPNHIAAIPRKKTLDIYLISDSSEVQDMTGSLMFIDSTGYITFHSVYH
                     GLKDSFHAPGQQSVQLASIPWDSLAGQTMLEFELSIPGWVVTYSTVYFIRPPNEASLK
                     PAAITQQYDPLRQTIYLDTDVPAWGVYLYSDEGELKLSDNFFDMRPGYQRAIELEVVP
                     DGFTGEVKIRTLNDLMNK"
BASE COUNT          442 a          517 c          437 g          489 t
ORIGIN      
        1 tcaggatctg aacacaaagc cacaaagaat acacaaagga cacagaggtc ttttaacatt
       61 tcagagatac gactgacccc tcaccactca ctgctctact cctcactgca acactgctcc
      121 tcagggtacc agcaccagcc cttccaggtt ctcctgactg aacacctcca gggtgtcgag
      181 attcagtgcc accagatggc ctttgaaatg atcgccggaa taaacacagc cacagtctat
      241 gttaaagacc atgcatttct cattttttac atctgcctcc aggtagtgta aggcccgtgg
      301 tgtgtgacca tgcacgatct ttctgttgcc tgttacctgg gtgtccatgc ttttctgcca
      361 gtcacgtatc cacagcatgg ctgtataatc gctgagcgga tcctcatcgc ggaagttgag
      421 tccggcatgc accagaatgt agtcttccag ttcaatgata tactgaagat ttctataaaa
      481 tttagcgtga tctgttggca gggccgacag aaaatcgtct tcctgaatgc catagctctg
      541 cagggtgatg gcgccaccgt tcctgctcag ccagttgatc aggcgactat catcatacgg
      601 tgcattcagc aacagccatt cgtggttacc cagcagacat tccactttgt aaccatcggc
      661 ctgcagttgc agtactttgt ccactacttc cttactgccc ggtccgcggt caatgagatc
      721 gcccagcaaa tacagggtat cgtctttcgt gagggctatc tgttccagca gtgcttcaaa
      781 ggtcttcgca caaccgtgta tgtctgaaat tgcgaatctg ctcatcttac ttgttcatca
      841 gatcattcag cgttctgatc tttacttctc ctgtaaaacc atccggtacc acttccagtt
      901 caatagctcg ctggtaaccc ggacgcatat cgaagaaatt atcgctgagt ttcagttctc
      961 cttcgtcgct gtaaaggtac acaccccagg ccggcacatc ggtatcgaga tagatggttt
     1021 gtctgagcgg atcgtattgc tgggtgatgg cagcgggttt cagggaagct tcgtttggcg
     1081 gacgaataaa ataaaccgta ctataggtaa cgacccatcc gggaatggac agttcgaatt
     1141 ccagcatggt ctgtcctgcc agcgaatccc atggaatgga agccagctgc acactttgtt
     1201 gtcccggtgc gtggaaggaa tccttcagtc cgtgatagac cgaatgaaag gtgatgtacc
     1261 cggtgctgtc aatgaacatc aacgaaccgg tcatgtcctg tacttccgaa ctgtcgctta
     1321 tgagatagat atccagcgtc ttctttcggg ggatggctgc aatatggttc gggccgaaag
     1381 ttcgtcgggc ggcatagtac aaagcctttc gtcttcccgc atgatccacc gcactccagc
     1441 tcgtgaccgg ccagcagtcg ttccattgcc agaataaagt acccatacac actggcatcg
     1501 ccgaaaggtg cgcatcaaat gcctgctgta tgccccaggc ctgcagcagc tgactcacat
     1561 accagtagtc ttccaggttg tccggcacgg ggaaatcacg ctgcatgtat tcgtctatgg
     1621 tctcgtagcc tacaggatgc ttctgatggg tcttcatcga cggtgataac agtttcagtt
     1681 gtgaactgtc catcgtctcc tgcagggtga accacgacgg cattccctga aatccgtatt
     1741 cactcatgaa atgccctgtc ttatccttcc agacagaaaa aggctccttc ccccaccata
     1801 caccccagta atgcatatcg ccttgtgtga ggctttcttt tcgtccccag cctatcgatg
     1861 gagaactggg ccagtaaggt cttcc
//
LOCUS       DAOMAX010000418         1881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15628_length_1881_cov_3.096933, whole genome shotgun sequence.
ACCESSION   DAOMAX010000418 DAOMAX010000000
VERSION     DAOMAX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15628_length_1881_cov_3.096933"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PK742_13470"
     CDS             complement(<1..719)
                     /locus_tag="PK742_13470"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQU77649.1"
                     /translation="MSFPTDIEIAQKAAIRPITEIAAKLNISSDDLELYGKYKAKLPL
                     SLIDEEKIKKAKLILVTAINPTPAGEGKTTVSIGLHEGMNKIGKKSVVVIREPSLGPV
                     FGIKGGAAGGGYSQVIPMEDINLHFTGDFSAVEKANNLLAALIDNNLNNKENLGIDPR
                     TVVWKRVMDMNDRALRQIVIGLGGKTNGVPREDGFNITPASEVMAILCMSQSFADLKQ
                     RLGNIYIGQTYDRKPVFARDL"
     gene            complement(993..>1881)
                     /gene="moaCB"
                     /locus_tag="PK742_13475"
     CDS             complement(993..>1881)
                     /gene="moaCB"
                     /locus_tag="PK742_13475"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HQU77650.1"
                     /translation="SYRMAVAQAVVSVSREETIIAIREGKVPKGNVFEMSRAAGLLAI
                     KNTSNVIPDCHPLPVEGASISHDIQGLDILISVQVETVYKTGVEVEAMHGASIVALTI
                     YDMLKPIDKQVSISNIRLLEKKGGKSDFKDQLARNIRTAVVVCSDSISSGKKEDKAGK
                     AIIEKLEPLQVEVEHYTIISDDKDVIQQKVRELIAGGTELLLITGGTGLSKRDITPEA
                     VGPLLERDIPGMMEAARAYGQQRMPYAMFSRGVAGMIGDSVVITLPGSTRGAIETMDA
                     LFPYVLHLFRVSAGMRHDG"
BASE COUNT          420 a          470 c          477 g          514 t
ORIGIN      
        1 ttcaagtcgc gggcgaagac aggctttctg tcgtaggtct gtccgatata aatatttccc
       61 agacgttgtt tcagatcggc aaagctttgc gacatacaca ggatggccat gacctctgaa
      121 gcaggagtga tgttgaatcc atcttcacgc ggaacgccat tggtcttacc gcccagtccg
      181 atcacgatct gacgaagggc ccggtcgttc atgtccatga cccgcttcca gacgacggtt
      241 cgcggatcga tgcccaggtt ctctttgttg ttgaggttat tatcgatgag tgctgccagc
      301 aggttgttgg ctttttccac ggctgaaaaa tcgccggtga aatgcaggtt gatgtcttcc
      361 atcggtatca cctggctgta tccgccaccg gcggcaccac ccttgatacc gaacaccgga
      421 ccgagagatg gttcacggat caccacgaca gatttcttcc cgatcttgtt catgccttcg
      481 tgcagaccga tggatacggt ggtcttgccc tcgccggcag gggtggggtt gatggcggtg
      541 accagaataa gtttggcttt tttgatcttc tcttcgtcta tcagactcag aggcagtttc
      601 gccttgtatt taccatagag ttcgaggtca tctgaggaga tgttcagttt ggctgcgatc
      661 tcagtgatgg gcctgatcgc agccttctgt gcgatctcga tatcggttgg aaaagacatt
      721 gggttgttgt ttggaaacaa atgtaggaaa atgagttgat tagccgatga gccgagaaaa
      781 tgtgaaaatg tgaaaatgtg aaaatgtgga aatgacgaaa tggggaaaag ctgggatgtg
      841 aaatcggggt ggaatttgca gggtatatct ggcttatgtg tccaggcaaa gcataaaccc
      901 aggctactgg aataaacaaa aatgaagtat ttgcatatta aattcttctg ccaattgctt
      961 cattctcaca tttccatatt tgcacatctt cttcacccat catgccgcat acccgcactg
     1021 accctgaaca aatgcaggac atagggaaac aatgcatcca tggtctcgat agctcctctg
     1081 gtgctgccgg gcagggtgat gaccacactg tcgccgatca tgcctgccac accccgtgaa
     1141 aacatcgcat aaggcatccg ttgctgtcca taagcacggg ctgcttccat cataccgggg
     1201 atatctcttt ccagcagcgg gccaactgct tccggtgtga tgtcccgttt agacaatccg
     1261 gtgcctcccg taatgagtag cagttcggta cctccggcga tcagttcacg tactttttgt
     1321 tgaatgacat ccttatcgtc ggagatgatg gtatagtgtt ccacttccac ctggaggggt
     1381 tccagttttt cgatgatggc cttgccggct ttgtcttcct tctttccgga agagatgctg
     1441 tcggaacaga ccaccacggc ggtccggata ttcctggcaa gctggtcttt gaaatcgctc
     1501 ttgccgcctt tcttttcaag taaacgaata ttggagatgc tcacctgttt gtcgatgggt
     1561 ttgagcatat catagatggt cagcgccacg atggaggcac catgcatggc ttctacttcc
     1621 acacctgttt tgtagaccgt ttccacctgt acgctgatca gaatatccag gccttgtata
     1681 tcatggctga tagatgcacc ttccaccggc agcgggtgac aatcgggtat gacgttggag
     1741 gtatttttga tggccagcaa tcccgccgca cgactcattt cgaatacatt ccccttcggc
     1801 acttttcctt ctctgatcgc tatgatggtc tcttccctgc tcacactgac aacagcctgc
     1861 gccactgcca tgcgatagga g
//
LOCUS       DAOMAX010000419         1860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_15925_length_1860_cov_4.535180, whole genome shotgun sequence.
ACCESSION   DAOMAX010000419 DAOMAX010000000
VERSION     DAOMAX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15925_length_1860_cov_4.535180"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1860)
                     /locus_tag="PK742_13480"
     CDS             complement(<1..>1860)
                     /locus_tag="PK742_13480"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77651.1"
                     /translation="FTYLWDFGDGTTSTLENPSHEYATFGFYDVTLTATNTYGCTTTF
                     TADDFVQIDPEVAIDFVPSSYAVCLGDTVFFDNLAGSAFGDWTWDFGDGSTSTDFEPF
                     VVYDAVGTYSVSLDAYFGLGCSGAISYPSLITVGAAPTVSFTSTDTYSACELPFAVSF
                     TPVVTGVGPFDYFWEFELPDGTFATATAPFPTYSWTAPGTYDVTLTVTSSSGCVVSYT
                     ASDLVTIDTLVITPEALPSQGCIPFSTEFVATATEDLVSWHWFFGDGASSFAENPSHT
                     YYDDSCYTVTFIGTTVNGCTDTVVLEDYVCAGFTGAAGGVSVPDTSCPGVPLDLDIFP
                     VDSIIGYIDGTGATSYTDFIDSLTFLDLPIGFHSIELITYSSGCPDTLYFDIFIVDVS
                     DTSLGTDYSCLEPLTVYFSMDTLLADSSCGWLWHFGDGETDTTLNPIHIYDEPGIYET
                     YAELFCVSLASCVGGTRELILTDPIALFEMPQMSCDTPFTIDFVNLSSDGVSETLDNL
                     WNFDDGSADTTEAPSHTFTDYGQYIVSLTITDTAECTAQFLDTLIINKLEAFFEPSET
                     TACVPAYVNLDDGSSSMFGTIQSWVVDWDDGEVDTFYSAAEMEAALHFYDTKAA"
BASE COUNT          549 a          441 c          472 g          398 t
ORIGIN      
        1 gctgctttcg tatcataaaa gtgcagtgca gcctccattt ctgcagctga atagaaagta
       61 tccacttcgc cgtcatccca gtcaaccacc cagctttgaa tggtgccgaa catactggat
      121 gagccatcgt ccaggttcac atatgccggc acacaggctg tggtttcaga aggttcaaag
      181 aatgcttcca gcttattgat gatcagtgta tccagaaact gtgcggtaca ttctgcagta
      241 tctgtgatgg taagagacac gatgtactga ccataatctg tgaaggtatg agaaggtgct
      301 tcggtggtat ctgcagaacc atcatcaaaa ttccagagat tatccagtgt ctccgagacc
      361 ccatcggacg aaaggttgac aaaatcgatc gtaaagggtg tgtcgcatga catctgcggc
      421 atttcgaaca aggctatcgg atcggtaagt atcagttccc ttgtgcctcc gacacaagag
      481 gccaggctca cgcaaaacag ctcggcataa gtctcgtaaa taccaggttc atcatatata
      541 tgtatcggat tcagggtggt gtctgtttcc ccgtcgccaa aatgccacaa ccatccgcac
      601 gacgaatccg ccagcaacgt atccatactg aaatagaccg tcaggggttc caggcaggag
      661 taatcagttc caaggctggt atcacttaca tcaacaataa atatatcaaa atacaatgta
      721 tcaggacacc ccgagctgta ggtgatcagt tcgatggaat ggaatcctat gggcagatcc
      781 aggaaggtca gcgaatcaat gaaatctgta taggaggttg ctccggtacc gtctatgtat
      841 ccgatgatgg aatcaacggg aaaaatatcc agatcaagcg gtacccctgg acaggaagta
      901 tcaggcacag acaccccgcc tgcggctcct gtgaagccgg cacagacgta atcttccagc
      961 actacggtat ctgtacagcc attcactgta gttccaataa aggtgaccgt atagcaggaa
     1021 tcatcataat aggtgtgcga tggattctct gcaaaggagc tggctccgtc gccaaagaac
     1081 cagtgccagc ttaccagatc ttcggtagcg gtagccacaa actcagtaga aaagggaata
     1141 caaccctgac tgggcagtgc ttccggagta atgaccaggg tatcaatggt aaccaggtct
     1201 gaagcagtgt agcttaccac acaaccactg ctgctggtaa cggtcagagt aacatcatag
     1261 gttccgggtg ctgtccagga ataggtagga aacggagcgg tagcagtggc gaaggtgcca
     1321 tcaggcagtt caaactccca gaaataatcg aaaggtccca cgccggtcac aaccggagta
     1381 aatgaaacgg cgaagggcag ttcacaggca gaataggtat ctgtgctggt aaaggaaaca
     1441 gtaggtgctg caccaacggt gatcaatgat ggataggaaa tggctccgga acatccaaga
     1501 ccaaaataag cgtcgaggct gacggaataa gtacccactg catcatagac cacaaacggc
     1561 tcaaaatctg tggaggtaga tccatcgcca aagtcccagg tccagtcgcc gaaagccgat
     1621 cctgccaggt tatcaaagaa cacggtgtca cccagacaaa cagcatagga agaaggcaca
     1681 aagtcgatgg ccacttccgg atctatctgt acaaagtcat cggcagtgaa ggtggttgta
     1741 cagccatagg tattagtggc agtaagtgtt acatcataaa atccgaaggt ggcatattca
     1801 tggctcgggt tctcaagggt agaggtagtt ccatcgccaa aatcccagag gtaggtgaac
//
LOCUS       DAOMAX010000420         1848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16128_length_1848_cov_3.348020, whole genome shotgun sequence.
ACCESSION   DAOMAX010000420 DAOMAX010000000
VERSION     DAOMAX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16128_length_1848_cov_3.348020"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PK742_13485"
     CDS             <1..795
                     /locus_tag="PK742_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HQU77652.1"
                     /translation="SATDDLSNESVYPSCDVMVIPVGSTPLQVVINEVMSSNITTIQD
                     EAGDFEDYIELYNSGNTTISLYGYYLSDDPAKPWKWQLPNTTMAPDSYILIWADDEGD
                     EGDYHAGFKIDVDGETIAMYGPSADGYPLVEQVEVPSLDDDISWGRLPNGTGEFTFLP
                     EPSPGKNNETGEPGNDPTPEPDRPYLTNNPSKSYSELRFDATGSLYYEVWMSDASGRN
                     LGRIYSGNPDEGHVNVGISTWPLPSGTYFLRLVTPASSQSFVLVVL"
     gene            1221..>1848
                     /locus_tag="PK742_13490"
     CDS             1221..>1848
                     /locus_tag="PK742_13490"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQU77653.1"
                     /translation="MGRAFEYRKEKKFKRWDAMAKNFTRVGREIAIAVKAGGPDPATN
                     AKLRLALNNAKGVQMPKDRVEAAIKRAVSKDEGDFEEITYEGYGPFGVAMMIDCATDN
                     TTRTVANIRMYFSRGDGAMGTQGSVAFSFDRKGLFKLEKDKLNMEEMELELIDGGAED
                     ISEEDDLWVVSTSFTDFGTMQNKLEELGVEAKSAELIRVPLNKVELTDD"
BASE COUNT          492 a          468 c          495 g          393 t
ORIGIN      
        1 agtgcgacag atgatctcag taatgaatcc gtgtatcctt cctgcgatgt catggtcata
       61 cctgtaggta gtacgccatt gcaggtggtg atcaatgagg tgatgtcgtc caacatcacg
      121 accatccagg acgaagctgg agactttgaa gattacatag aactctataa cagtggcaac
      181 accaccatca gtttatatgg ttattacctg agtgatgatc cggccaaacc gtggaaatgg
      241 caactcccca acaccactat ggcacccgat tcttatatcc tgatctgggc agatgatgag
      301 ggtgatgaag gcgattacca tgcaggtttc aaaattgatg tggacgggga aacgatcgct
      361 atgtatggtc cttcggccga tggctatccg ctggtggaac aggtggaagt tccttctctg
      421 gacgacgaca tcagctgggg aagactgccg aacggaacgg gcgaattcac atttcttccc
      481 gagcccagtc ccggcaagaa caacgagacc ggagaacccg gcaacgaccc tacacccgaa
      541 cctgatcgtc cctacctcac caacaatccg tccaaatctt attcagaatt gcgttttgac
      601 gccaccggca gtctttacta cgaggtctgg atgagcgatg ccagcggacg aaatctgggc
      661 cgcatttatt ccggcaaccc tgatgaagga catgtcaacg taggtatcag cacctggccc
      721 cttccctccg gcacctattt cctgcgactg gtaacaccgg cttccagcca gagtttcgtg
      781 ttagtggtgc tgtaaacaga atacaagaaa atccaggcag gtagctgcca tttcttctgc
      841 aacagaggaa acagaggaac catttcccct gccggcagtg cgagggctga agcatcccca
      901 gcgcaaggtc ttgctttgcg ttccgaagcg ccaatctaaa tctggtttag aaagcggtgt
      961 ctgacaaagt ctgtcggagc ccccaggccg atgcgatgaa ggaatgcccg gcgccgcagg
     1021 atgcgttgca gggccgcaaa agcaccttcg gcaggggaaa gcgttttggt gatacctttt
     1081 gcgctcaaaa ggtatcgaaa cctcattgga gtgatccggt ttagctggaa ttggcctgat
     1141 tatttgagta ggcttataaa gtaaatatcc taaaagaggc atctgctagt gccttctctt
     1201 aattattttt gcagcgcatt atgggaagag cattcgaata ccgcaaagag aagaagttca
     1261 agcgctggga cgccatggcc aagaacttca cccgtgtagg gcgggagatc gctatcgcag
     1321 tgaaagccgg cggaccagac cctgccacca acgccaagct gcgactggca ctcaacaacg
     1381 ccaagggtgt gcagatgccc aaagaccggg tggaagccgc catcaaacgg gccgtgagta
     1441 aagacgaagg cgatttcgaa gagatcacct atgaaggtta cggaccattc ggtgtggcca
     1501 tgatgataga ttgcgccacg gacaatacca cccgaacagt agctaatatc cgcatgtatt
     1561 tcagccgcgg ggatggtgcc atgggtaccc agggttctgt ggcattctcc ttcgaccgca
     1621 aaggcctgtt caagctggag aaggacaagt tgaacatgga agagatggag ctggaactga
     1681 tagacggcgg agcggaagac atttcagaag aagacgatct ctgggtagta tccacctcct
     1741 tcaccgattt cggcaccatg cagaacaaac tcgaagaact gggtgtggaa gcaaaatcag
     1801 cagagctcat tcgtgtaccc ctcaacaaag tggaactgac cgacgatc
//
LOCUS       DAOMAX010000421         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16182_length_1845_cov_3.468156, whole genome shotgun sequence.
ACCESSION   DAOMAX010000421 DAOMAX010000000
VERSION     DAOMAX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16182_length_1845_cov_3.468156"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1753)
                     /locus_tag="PK742_13495"
     CDS             complement(113..1753)
                     /locus_tag="PK742_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HQU77654.1"
                     /translation="MSKTDTNILHTEPVIFPHKEDIPENARLKLPIHQTVWLVNGELR
                     EWKGPFNTVLSPVCMKSAKGLERVEVGSHPMLTKEAAVEAVESAFNAYAGGRGLWPTL
                     TVKKRVESMLLFVQGMEQKREEVVRLLMWEIGKTRKDAEKEFDRTVQYIYDTMEELKD
                     VNRENAQLQKEEGIYAQIRRSPLGVALCMGPYNYPLNETFATLIPAIIMGNTVVFKPA
                     RFGVLLIQPLLELFREHFPKGVVNVIYGEGKEIIGPIMETGKIDVFAFIGTSRVANII
                     KRQHPKPNRLKSVLGLEAKNPAIVLPDADIDNAVAECLSGSMSFNGQRCTALKILFVH
                     ESIVAKFNEQFIAALNKLQPGMPWEDKVGITPLPEKDKPAYLKELVDDAVQKGATILN
                     EGGGAMQESYFHPAVLYPVSSDMRVWEEEQFGPVIPIVPFRSVSEPLEYIDESNYGQQ
                     VSIFGKDPNRLVELIDPLVNLVCRVNLNSQCQRGPDVYPFNGRKDSAVATLSVHDALR
                     VFSIRTTVAFKDLPMNKQIVTNVLEGRKSNFLSTDYIL"
BASE COUNT          384 a          497 c          463 g          501 t
ORIGIN      
        1 acatgaacca cagagttcct tggcaggtaa ctctgtgggg acaaaagagc attctctgtt
       61 gtacagagag ttcttctcac ccctcacttc ccgcttcggc gggattcacc tctcacaata
      121 tataatccgt gctcaaaaag ttcgacttcc ttccttccag cacattggtc acgatctgct
      181 tgttcatggg caggtctttg aaggctacgg tggtacggat actgaacacc cgcagcgcat
      241 cgtgcacact cagggtggcc acggcagaat cttttcttcc gttgaaggga tagacatccg
      301 gtccccgctg acactggctg ttgagattga cacgacacac gaggttgacc agcggatcga
      361 tgagttccac cagcctgttc gggtctttgc cgaagatgct gacttgctgc ccgtagttgg
      421 actcgtcaat atactccagc ggttcactta cggaacggaa gggtacgatg ggaatgaccg
      481 gtccgaactg ctcttcctcc catacccgca tatcgcttga aaccggatac aacacggccg
      541 gatggaaata cgattcctgc atggcgccgc ctccttcatt tagtatagtg gcgccttttt
      601 gcacagcgtc atccaccagt tctttcaggt aggccggctt gtctttttcc ggaagtggtg
      661 tgatgcctac tttgtcttcc cagggcatgc ctggttgcag tttgttgagt gccgcgatga
      721 actgttcatt gaacttcgct acgatgctct catgcacgaa caggatcttg agtgcggtgc
      781 accgctgtcc gttgaacgac atgctgccgc tcagacattc ggctacggca ttgtcgatat
      841 cagcatcggg caatacgatg gcgggatttt tggcttccag ccccagcacc gatttcagcc
      901 tgttgggttt gggatgctgg cgcttgatga tattggctac ccggctcgtt ccgatgaaag
      961 cgaacacgtc tatcttgccg gtctccatga tgggaccgat gatctctttt ccttcgccgt
     1021 agatcacgtt gaccacgccc tttgggaaat gctcgcggaa taattccagc aacggctgaa
     1081 tgagcaacac accgaaacgg gcgggcttga acaccaccgt gttgcccatg atgatggccg
     1141 gtatcagcgt ggcgaaagtt tcgttgaggg gatagttgta gggacccata cagagagcaa
     1201 cacccagcgg actccttcgg atctgcgcat agatgccttc ttccttctgc agctgggcat
     1261 tctcgcggtt cacatccttg agctcttcca tggtgtcgta gatgtactgc acggtgcggt
     1321 cgaattcttt ttcggcatcc ttgcgggtct tgccgatctc ccacataagc agtcgcacca
     1381 cttcttcccg tttctgttcc atgccctgca cgaacaacaa catgctttcc acccgctttt
     1441 tcacggtcag tgtgggccat aacccgcggc caccggcata ggcgttgaag gcagattcca
     1501 ctgcttctac cgctgcttct tttgtcagca tggggtgact gcctacttcc actctttcca
     1561 gtcctttagc ggacttcata cacacgggcg ataggacagt attgaacgga cccttccatt
     1621 cccgtaactc tccgttgacc agccatacgg tttgatgaat cggaagtttc aggcgagcgt
     1681 tttcaggaat atcctccttg tgcggaaaaa taacaggttc tgtatgtaat atattggtat
     1741 ctgttttact catatgttat aattgtgtaa aagaggccga gtagtttttt tctccatcct
     1801 gcagatggat gaaataacgg cctggtgaca atgtttgtgt gttca
//
LOCUS       DAOMAX010000422         1841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16254_length_1841_cov_3.622620, whole genome shotgun sequence.
ACCESSION   DAOMAX010000422 DAOMAX010000000
VERSION     DAOMAX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16254_length_1841_cov_3.622620"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PK742_13500"
     CDS             complement(<1..313)
                     /locus_tag="PK742_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77655.1"
                     /translation="MIADFIKGKRILLAALISGIAIAAIYAGLQYLIPDTQLYMLTGL
                     LVAWLTGLLLLMWRELHVHIRKQTLERHRWQEAWDGIRDILTFYHPLPPSTTFTIAPD
                     AA"
     gene            complement(310..1341)
                     /locus_tag="PK742_13505"
     CDS             complement(310..1341)
                     /locus_tag="PK742_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77656.1"
                     /translation="MRKAVIAFLYPSHYPVSGGSSMHGYFLARELSALGYRLISFPRT
                     PDGFSKCLPYRPWFVLWAILTSDLVYLRISPEGKSTRLLKWLKLLRKPHVVELNGPSD
                     ELRVTKGYTLEQIEQMDRELATSLTAARAVITVSEVMQRWCKQYLQLEHVYVIENGGE
                     QYDARSLRPDASLMQQLDDFSNGGSQLILWSGNAYPWQGVDEICKLIETASPDMRFVL
                     VSDSVSAFEEVATRNNVLLLPTQSQENMRYLITQAHAGLALYGDFSWFRLGEMYNSSL
                     KLYEYLANGLWVVTNYPLFGSAQLYVGKEASDMIAWLNGKKEEFVSPGQPYRSWRQVA
                     LETAAILEK"
     gene            1403..>1841
                     /locus_tag="PK742_13510"
     CDS             1403..>1841
                     /locus_tag="PK742_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77657.1"
                     /translation="MKTYFLDIHWYDYGLYLVYILLFVYLLHRIGRVAYGLSSLSVKL
                     QMFTFLLYAAVLIADSFLDFLPFLPDTGMYTYMIKTGLYPDTTSVNILALYYFSIVIA
                     ILCLNSPIIFTFFNVFFFLVAVQFMMSRFPGEDTKLKDRQQTVF"
BASE COUNT          452 a          491 c          415 g          483 t
ORIGIN      
        1 aagctgcatc aggcgcaatg gtgaaagttg tagatggagg cagggggtga tagaaagtca
       61 ggatatcacg gataccatcc caggcttcct gccatctgtg ccgctccagc gtttgctttc
      121 tgatgtgtac atgaagttcc ctccacatca ggagcagaag gcctgtcaac caggctacca
      181 gtaaccctgt aagcatatac agttgggtat cgggtatcag atactgaagc cctgcatata
      241 tagctgcaat tgcaatacca ctgatcaacg ccgccaggag tatccgtttc cctttgatga
      301 aatctgctat catttttcca gtattgctgc tgtttccagt gctacctgtc gccagcttcg
      361 ataaggctgc cccggcgata caaattcttc cttttttcca ttcagccagg cgatcatatc
      421 tgaagcttct ttacccacat acagctgagc cgagccgaac aaggggtaat tggtgaccac
      481 ccacaaacca tttgcaaggt actcatataa tttaaggctg ctgttgtaca tctcacccag
      541 cctgaaccag gaaaaatcgc catagagagc cagccctgca tgtgcctgag tgatcaggta
      601 acgcatattc tcctgagact gcgttggaag cagcagcacg ttgttccggg tggccacttc
      661 ttcaaatgcg ctgacagagt cactgaccaa tacaaaccgc atatcgggag aggctgtttc
      721 tatcagtttg cagatctcat ccactccctg ccacggatag gcattaccgc tccataaaat
      781 gagctggctt ccaccatttg aaaagtcatc cagctgttgc ataagagacg catcaggccg
      841 aagtgaacgg gcatcatatt gctcgccacc gttttcgatc acatacacat gctccagctg
      901 gagatattgc ttgcaccatc tttgcatcac ttcactcacg gtaatgacag ctctggcagc
      961 ggtgagtgag gtagccagtt ccctgtccat ctgctcgatc tgctccagag tgtatccttt
     1021 ggtgacacga agctcatctg atggcccgtt caattctacc acatgcggtt tgcgtaacag
     1081 tttcagccat ttcagaagcc gggtgctttt accttcagga ctgatgcgga ggtacaccag
     1141 atcggaggtc aaaatagccc acagcacaaa ccagggacgg taaggcaggc atttactgaa
     1201 gccgtcaggt gttctgggga aagagatcag gcggtaaccc agcgcactta actcgcgggc
     1261 tagaaaatac ccgtgcatgc ttgacccgcc gctgaccgga tagtgcgatg ggtacaaaaa
     1321 agctatgacc gctttacgca tcagggcgaa agtaataatt ttacacacct caacctgccg
     1381 ggcaacaacc aaactacctg atttgaaaac ctattttctt gacatccact ggtacgacta
     1441 cggactgtac ctggtttaca tcctcctgtt tgtgtacctg ctgcaccgca tcggccgggt
     1501 ggcctacggg ctttcttccc tctctgtgaa acttcagatg ttcacctttc tgctgtatgc
     1561 tgctgtacta attgccgaca gctttctgga tttcctgccg tttctgccag ataccggtat
     1621 gtacacctat atgatcaaaa cgggattgta tcctgatacc acctcggtaa atatcctggc
     1681 cctgtattat ttcagtatcg ttattgccat actctgtctg aacagcccga tcattttcac
     1741 ctttttcaat gtctttttct ttctcgtggc agtgcagttc atgatgtccc gttttccggg
     1801 cgaggataca aaactcaaag acagacaaca aaccgtattt g
//
LOCUS       DAOMAX010000423         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16528_length_1824_cov_3.979084, whole genome shotgun sequence.
ACCESSION   DAOMAX010000423 DAOMAX010000000
VERSION     DAOMAX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16528_length_1824_cov_3.979084"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK742_13515"
     CDS             complement(<1..615)
                     /locus_tag="PK742_13515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQU77658.1"
                     /translation="MQLPHNRYIPWLLALVTGVVLLPGLGVAHLFDWDEINFAEIARE
                     MTVSGNYSQPTMRFLPFWEKPPLFFWLQALGMEVFGVGEFAARLPNVICGMITIPLLY
                     TIGRRLFDETFGMIWAMAYLGALLPGIYFQSGIIDPVFNLFIFLSLVQFVRYVWKRSR
                     KHPELAGRHALSYLIWAAVFAGLAVMTKGPAALLVISLCMAVFWV"
     gene            complement(643..1782)
                     /locus_tag="PK742_13520"
     CDS             complement(643..1782)
                     /locus_tag="PK742_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQU77659.1"
                     /translation="MEFQLTDDHLMIRQAARDFAQQVLKPGVIERDRDMKYPTEEVKQ
                     MAELGFLGMMVSPEYGGGGMDTVSYVLAMEEISKVDSSCSVIMSVNNSLVCWGLEKYG
                     TEEQKQKYLPDLATGKKIGAFCLSEPEAGSDATSQRTTAEDKGDHYLLNGTKNWITNG
                     GTASVYLVMAQTHPELGHKGINCLIVERGMDGFVVGAKEDKLGIRASDTHTLIFNDVK
                     VPKENRIGDDGFGFKFAMKTLTGGRIGIASQALGIASGSYELALEYAKTRKAFGKEIA
                     QHQAIQFKLADMATEIEAARLLVMKAAWLKDNHMDYDIASSMAKVFASETAMRTSVEA
                     VQIHGGYGYVKEYHVERLMRDAKITQIYEGTSEIQRIVISRGVLK"
BASE COUNT          439 a          504 c          432 g          449 t
ORIGIN      
        1 gacccaaaac acagccatgc agagcgatat taccaacagt gctgccggac ctttggtcat
       61 aactgcaaga cctgcgaaaa cagcagccca tatcaggtaa gacaaggcat gtctgccggc
      121 aagttccgga tgttttcgtg accgcttcca cacatagcga acgaattgca ccaggctaag
      181 aaagatgaac aggttgaaga ccgggtcgat gatgccactc tggaaataga tcccgggaag
      241 caaagcaccg agataggcca tcgcccagat cattccgaat gtttcatcga ataatcttcg
      301 gccgatggtg tacaataatg gaatggtgat catgccacag atcacattcg gcaatcttgc
      361 agcgaattca cccacaccga acacttccat ccccagtgcc tgcaaccaga aaaataaagg
      421 gggtttttcc caaaagggaa ggaaacgcat ggtgggctgt gagtaattgc cactcaccgt
      481 catctcgcgg gcgatctcgg caaagttgat ctcgtcccag tcgaacaggt gcgccacgcc
      541 cagaccgggc aataacacca caccggtaac cagggccagc aaccagggta tgtatcggtt
      601 gtggggaagc tgcatctccc aaaaataagc gggatggatg gtttacttca gcacacctct
      661 ggaaatgacg atgcgctgga tctcactggt gccttcatag atctgggtga tcttggcatc
      721 acgcatgagg cgctccacat ggtattcctt gacatagccg taaccaccgt gtatctgcac
      781 tgcttccaca gaggtgcgca tggcagtttc actggcaaac accttggcca tactggaggc
      841 gatgtcgtag tccatatgat tgtctttcaa ccaggccgcc ttcatgacca gcagtctggc
      901 agcttctatc tctgtagcca tgtctgccag tttgaactgg atggcctgat gctgagcgat
      961 ctccttaccg aaagccttcc tggtcttggc atattccaga gccagttcat aagaaccgga
     1021 tgcaataccc agtgcctgtg atgcaatccc gattctgcca ccagtcaggg tcttcatggc
     1081 aaatttgaat ccgaaaccat catcgccaat gcgattttcc ttcggcactt tcacatcgtt
     1141 aaagatgagc gtatgggtat cagaagcacg gatgccgagt ttatcttcct tcgcccctac
     1201 cacaaaacca tccatacctc tctccacgat caggcagttg atgcctttgt ggcccagttc
     1261 cggatgggtc tgtgccatga ccagatatac agaagcggta ccaccgttgg tgatccagtt
     1321 cttggtaccg ttgagcaggt aatgatcgcc tttgtcttcg gcagtggtac gctggctggt
     1381 agcatctgaa ccggcttcgg gctcactcag acagaacgca ccgatctttt tgccagtagc
     1441 cagatccggc agatatttct gcttctgttc ttccgtaccg tatttctcca gtccccagca
     1501 cacgaggctg ttgttcacgc tcatgatgac cgagcaggag ctgtccacct tgctgatctc
     1561 ttccatggcc aggacatagc tcacagtgtc cattccgcca ccgccgtatt cagggctcac
     1621 catcataccg aggaaaccca gctctgccat ttgcttcact tcttcggtgg gatatttcat
     1681 atctcggtcg cgttctatca caccgggttt caacacctgc tgcgcaaagt ctcttgcagc
     1741 ctggcggatc atcaaatggt catctgtcag ttgaaattcc atatatttat tttttatctc
     1801 ctgatattag tgtcgtagtg gaat
//
LOCUS       DAOMAX010000424         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16687_length_1815_cov_4.796023, whole genome shotgun sequence.
ACCESSION   DAOMAX010000424 DAOMAX010000000
VERSION     DAOMAX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16687_length_1815_cov_4.796023"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PK742_13525"
     CDS             <1..1158
                     /locus_tag="PK742_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU77660.1"
                     /translation="LNIAYQDNHRQEYDLHRMSDGGPALDLSIGTALAEAEWKHRSFM
                     HTNGSIGISAMNQQNVWTGRFFIPNFISNTAGVFWMENWVSLPWKAEAGVRYDVRKLR
                     SFRNDGEAITETTRNFSHLSWSGGIGWQQERLSLSFNSGSAWRSPSINELYAAGLHHG
                     AAALEYGDPDLQIETSWQNSLSAKWESARLQIDLGAYVNLMDGFIYLEPQAPPALTIA
                     GAFPVFAYKQTNARLTGMDAKISYTLFREFRLEAGAAVLRAWDITNNDWIIQMPSDRL
                     TGGFHWHFHDGRDQGWDISAGITHILEQSRVPDTEDYAPPPAAYTLVRMEAGSELHWG
                     NTVVNWSLSADNLFNTAYRDYLDRYRYFADEPGRDISLHVFIPIQTKNKTS"
     gene            1155..1697
                     /locus_tag="PK742_13530"
     CDS             1155..1697
                     /locus_tag="PK742_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77661.1"
                     /translation="MKNTLIIAAAAAMFISSCKDAETPQPENESELITTVRLDLVNQS
                     DTTDVSTFYFRDNDGPGGLDPLQWDTVRLAAGTTYDLTIALLDESTLPNGDISAEVEE
                     EGTDHQLFFNIDPASLLAVDYNDSDDNGDPVGLVNVATTSSTGAGTLTVILKHQPGIK
                     DGNQNTGETDVEVEFVLEVL"
BASE COUNT          467 a          470 c          472 g          406 t
ORIGIN      
        1 ctgaacatcg cctaccagga caatcaccgc caggaatacg acctgcaccg gatgtctgac
       61 ggcggaccgg cactggacct cagcataggt acagcactgg ctgaagcaga gtggaagcac
      121 cgctccttca tgcacacgaa cggaagcatc gggatcagcg ccatgaacca gcagaacgtc
      181 tggactggac gattcttcat tccgaatttc atcagcaata cggccggtgt attctggatg
      241 gagaactggg tgagtcttcc ctggaaagca gaagctggtg tgcgatatga tgtgcgcaag
      301 ctgcgttcct tccgcaatga cggggaggcc attaccgaaa ccacccgcaa cttctctcat
      361 ctgagctggt caggtggtat aggctggcaa caggaaaggc tatcactgtc gttcaacagc
      421 ggttctgcat ggcgttcccc ttccatcaat gaattgtatg cagcgggact acaccatggc
      481 gctgcggctc ttgagtatgg cgatcctgat ctgcagatcg aaacatcctg gcagaacagc
      541 ctgtcggcga aatgggaaag cgccagactg cagatcgatc ttggtgctta cgtcaacctg
      601 atggatggct tcatctatct ggaaccacag gctcctcctg ccctgaccat tgccggtgca
      661 tttccggtgt tcgcctataa acaaacgaac gcacgattga ccgggatgga tgcgaagatc
      721 agttacacgt tgttccgcga attccgttta gaagcgggcg ctgctgtctt aagggcatgg
      781 gatatcacca ataatgactg gatcatccag atgccgtccg acagattgac cggtgggttc
      841 cactggcatt ttcatgatgg cagagatcag gggtgggata tcagcgcagg catcacccat
      901 atcctggagc agtcacgggt acctgataca gaagactacg ctccgccacc tgcagcctac
      961 accctggtgc ggatggaagc aggaagtgaa ctccattggg gaaacacggt tgtgaactgg
     1021 tcactctcgg ccgataacct gttcaatacc gcttaccgcg actacctgga cagataccgc
     1081 tattttgctg atgaacccgg aagagacatc agcctccatg tatttattcc tattcaaacc
     1141 aaaaacaaaa catcatgaaa aataccttga ttatcgctgc agctgcagcc atgttcattt
     1201 cttcctgcaa agatgcagaa acaccacagc ctgaaaatga atcggaactg atcactacgg
     1261 tcaggctgga tcttgtgaac cagtctgata cgaccgatgt atctaccttc tacttccgtg
     1321 ataatgacgg tcccggcgga cttgaccctt tacagtggga tacagtaaga cttgctgcag
     1381 gaactacgta tgatctgacc atcgcattat tggatgaaag caccttgccc aatggtgata
     1441 tctctgctga agtagaagag gaaggaactg atcatcagtt gttcttcaac atcgatccgg
     1501 cctctctgct tgcggtcgat tacaacgaca gcgatgacaa tggtgatcct gtcggactgg
     1561 tgaatgtggc cacaacttcc agcaccggtg caggaacctt gactgtcatc ctcaaacacc
     1621 agccgggcat caaagacggc aaccagaaca ccggcgaaac ggatgtggag gtggagtttg
     1681 tgttggaagt gttgtgaaaa tctcatttta aaggaacttg tcacccccct gcctaccggc
     1741 agggaatctc ataattaaca ggatgcattg agaaggagac tccgggtcaa gcccggagag
     1801 gcattcggct ggagt
//
LOCUS       DAOMAX010000425         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16742_length_1812_cov_4.735344, whole genome shotgun sequence.
ACCESSION   DAOMAX010000425 DAOMAX010000000
VERSION     DAOMAX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16742_length_1812_cov_4.735344"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PK742_13535"
     CDS             <1..719
                     /locus_tag="PK742_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HQU77662.1"
                     /translation="QTVIITVPNVSYNCFTTSGEKIVVHNPTDLPDPSYIEYVEEPYI
                     RAQIITKPDYIGAIMKLCMDKRGTLKNQVYLTTTRVEMVFDMPLAEIVFDFYDKLKSI
                     SRGYASFDYEITDFRQSDLVKMDVKLNGENVDAFSALIHRTKAYDFGSRLCKKLKDLL
                     PRQQFMIAIQAAIGAKIIARETISAMRKDVTAKCYGGDITRKRKLLEKQKKGKKKMRQ
                     IGTVEVPQQAFLAVLKLNED"
     gene            721..1614
                     /gene="folD"
                     /locus_tag="PK742_13540"
     CDS             721..1614
                     /gene="folD"
                     /locus_tag="PK742_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HQU77663.1"
                     /translation="METNTSPLILDGRTLSKTIREELSEQVASWMAGGGKQPHLAAIL
                     VGDDGASQTYVSSKVKGCEQTGMKSSLFRFPAETSEQEILDKIDALNNDHDIDGFIVQ
                     LPLPKHIDELKVTLAIDPAKDVDGFHPENAGRIQLGLPAFVSATPMGIMTMLERYQIE
                     TSGKHCVVIGRSNIVGTPMSILLSRNTNPGNCTVTLCHSRTPDIAAVCREADIIVAAL
                     GKPRFVTADMVKEGAVVVDVGITRVPADNDKGYVIAGDVDYDQVAPKCSAITPVPGGV
                     GVMTVTSLLMNTFQAAQRKSF"
     gene            1618..>1812
                     /locus_tag="PK742_13545"
     CDS             1618..>1812
                     /locus_tag="PK742_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77664.1"
                     /translation="MEGLPVMEQFLSIQGEGAHQGRAAWFIRLGGCDVGCVWCDVKES
                     WDVTAHPVVSVAEILAGIPEN"
BASE COUNT          520 a          406 c          453 g          433 t
ORIGIN      
        1 atcagacggt catcatcact gtaccaaacg taagctacaa ctgctttacc acttcaggcg
       61 aaaagatcgt ggtacacaac cccaccgatc tgccagatcc gtcttatata gaatacgtcg
      121 aggaacctta tatacgtgca cagatcatca ccaagcccga ttatattgga gctatcatga
      181 agctctgtat ggacaaacgg ggtacactca agaaccaggt atatctgacc accacccgtg
      241 tagaaatggt ttttgatatg ccacttgcag agatcgtgtt tgatttctat gataagctta
      301 aatccatttc acgtggttat gcatcgttcg attatgagat cactgatttc agacagagtg
      361 atctggtaaa aatggacgtg aaactgaacg gagaaaatgt ggatgccttt tcggcgctca
      421 tccaccgtac caaggcatac gactttggca gcaggttgtg taaaaagctg aaggacctgc
      481 ttccccgcca gcaattcatg atcgccatac aggctgctat cggagcaaag atcatagcac
      541 gggaaaccat ctcagcaatg agaaaagatg taacagcgaa atgttatggt ggcgatatta
      601 ccagaaaacg taaattgctg gagaaacaga aaaaaggtaa aaagaaaatg cgccagattg
      661 gtacagtgga agttcctcag caagccttcc tggcagtatt gaaactcaac gaagactgat
      721 atggaaacga atacctctcc cctgatcctg gatggcagaa cactgtcgaa gaccattcgt
      781 gaagaactgt cagaacaggt agcttcctgg atggccggtg gcggaaaaca acctcatctg
      841 gctgccattc tggttggcga tgatggtgcc agtcaaacct atgtatccag caaggtaaaa
      901 ggatgtgaac agaccggtat gaaatcgagt ctgttcagat ttccggcaga aaccagcgaa
      961 caggaaattc tggataagat cgatgcactg aataatgatc atgatattga tggtttcata
     1021 gtacagcttc cgctgccgaa acatattgat gaacttaagg ttaccctggc catagatccg
     1081 gccaaagatg tggatggctt tcatccggaa aatgccggac ggatccagtt aggtttacct
     1141 gcttttgttt cagccacacc aatgggcatc atgaccatgc tggaacgcta tcagatcgaa
     1201 acatccggga agcattgtgt ggtcatcggc aggagcaata ttgtaggaac ccccatgagc
     1261 atactgctgt ccaggaacac gaatccgggt aactgtaccg tcactctctg tcacagcaga
     1321 actccggata tagccgcagt ttgccgtgaa gctgatatca ttgtagccgc attaggcaaa
     1381 ccacgttttg tcactgcaga tatggtcaaa gaaggcgctg ttgtagtaga tgtaggcatc
     1441 acacgtgtac cggcagataa cgacaaaggt tatgtcattg ccggcgatgt ggactatgat
     1501 caggtagctc ctaaatgcag cgccattact cctgtgccgg gtggtgtggg cgtaatgacc
     1561 gttaccagtc tgctgatgaa tactttccag gctgcacaga gaaaatcatt ctgaaacatg
     1621 gaaggtcttc cagtcatgga gcaattcctc tccattcagg gagaaggagc gcaccaggga
     1681 cgggctgcct ggttcattcg cctgggagga tgtgatgttg gttgcgtctg gtgcgatgtc
     1741 aaagaaagct gggatgttac tgcccatccc gtggtttcag tagctgaaat actggcaggc
     1801 attccggaaa at
//
LOCUS       DAOMAX010000426         1810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_16775_length_1810_cov_3.705413, whole genome shotgun sequence.
ACCESSION   DAOMAX010000426 DAOMAX010000000
VERSION     DAOMAX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16775_length_1810_cov_3.705413"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(525..597)
                     /locus_tag="PK742_13550"
     tRNA            complement(525..597)
                     /locus_tag="PK742_13550"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Phe,seq:gaa)
     gene            complement(758..1717)
                     /locus_tag="PK742_13555"
     CDS             complement(758..1717)
                     /locus_tag="PK742_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQU77665.1"
                     /translation="MNRFYYLLGGFALGLAVLAILASTTSDGSRKIEESGTSNGALRK
                     LGEDPSLVRNYAVKIPEGLNFAGEKVPLSDEEVFERMDRELLVNSYWHSNTMQNLKLA
                     HRWFPVIEETFKEYGIPSDFKYIALAESGLRNVVSPAGAEGVWQFLPETARHFDLRVD
                     DEVDERYDVQKATVAAAKYFQTAHDRLGSWTLAAASYNAGIGKIENSLEYQEVDNYYD
                     LFLNNETSRYIFRILAFKVIYENTEKYGFLLDEEDLYDPLTYKTITVDTTITNLAAFA
                     TRLNTNYKTLKYYNPWLRDRKLTVKKGEEFRLYVPEDGSTSKE"
     gene            complement(1714..1803)
                     /locus_tag="PK742_13560"
     CDS             complement(1714..1803)
                     /locus_tag="PK742_13560"
                     /inference="COORDINATES: protein motif:HMM:NF022118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HQU77666.1"
                     /translation="MTKRLRPLAKSIMYGEPCSTVPLFKRRES"
BASE COUNT          426 a          436 c          423 g          525 t
ORIGIN      
        1 aggggggggg gtgttttttt gatctggcgg cacctgatgc ttgttagagc ctcaaattgg
       61 acctgatgag tgtgtcatgt taatagatcc aatcttgcag gtgactgaaa catttcacga
      121 ttctacacat cgacttatta taggcactgg atgatcacag aatcaaaaag aaaccaatcc
      181 tttaaagggg ggtgggttgg aatgatcttg ccattatttt gcatctcagt gtattctgac
      241 cttttgtttg aaaatatata ctgcattctc ggaaccgata gtcttttgaa agaatgaata
      301 gaacatcgat ttactagcat tttgaattgg cgcaccctgg tgactataga tcaagggata
      361 tattggtttt gcaaaattct aatgcaggcg ttaattggac atatgctgtc tgcgtaattg
      421 atcaggtttc aggtttttgc cgaaaatctg cccccctgac tgtcccccta aaaaagtcag
      481 ccctcataag tcattgactt acaagggctg acttctgttt gctgtggtgt ggggcggggt
      541 cgaaccgccg acacatggat tttcagtcca ttgctctacc aactgagcta ccgcaccata
      601 atttcaaata ccaatcccga atcgttcctc tcgggatcct gtttttctta ggttcgttcc
      661 ctcaccttga aaagccaaga ctgagcaacc gcaccattgt gttaattacg gagtgcaaaa
      721 atagcggaat tgctgtattt gtcaaggggt tagtgtatta ttcctttgaa gtactgccat
      781 cttccggcac atacagccgg aattcctccc cttttttcac cgtgagtttt ctgtcacgaa
      841 gccaggggtt gtaatacttc agcgttttat agttcgtgtt cagccgggtg gcgaatgctg
      901 ccaggttggt gatggtggta tctaccgtga tggtcttgta agtaagcgga tcatacaggt
      961 cttcctcatc cagcaaaaaa ccatacttct ccgtgttctc gtaaatgacc ttgaatgcca
     1021 ggatgcggaa gatgtagcgg ctggtctcgt tattaagaaa gagatcataa tagttatcta
     1081 cttcctggta ttcgaggctg ttctcgatct tgccgatgcc tgcattgtaa gatgctgccg
     1141 ccagggtcca gctgcccagt ctgtcgtggg cggtctgaaa atatttcgcc gctgccacgg
     1201 tggctttctg tacatcatac cgttcatcca cttcgtcatc cacccgcagg tcaaaatgcc
     1261 ttgcggtttc gggaagaaat tgccagactc cttctgcacc cgccggtgag accacatttc
     1321 tcagtccgct ctctgccagg gctatgtatt tgaaatctga cggaataccg tattccttaa
     1381 aggtctcttc gatgaccgga aaccagcgat gtgccagttt caggttctgc atggtgttgg
     1441 aatgccagta actgttgacc agcagttcac gatccattct ctcaaatacc tcttcgtctg
     1501 acagtggtac tttttcacct gcaaaattca acccctccgg tatcttgacc gcatagttgc
     1561 gcaccagact cgggtcttca cccagcttcc gaagagcacc atttgaggtg cctgattctt
     1621 cgatcttacg gctgccatca gaagtggtgg atgccaatat ggcaagtaca gccagaccca
     1681 gcgcaaagcc gcctaacaag taataaaacc tgttcatgat tcccgtcgtt tgaacagcgg
     1741 cacggtgctg cagggttctc cgtacatgat gcttttggcc agcggccgca ggcgtttcgt
     1801 caactccaga
//
LOCUS       DAOMAX010000427         1794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_17037_length_1794_cov_2.985624, whole genome shotgun sequence.
ACCESSION   DAOMAX010000427 DAOMAX010000000
VERSION     DAOMAX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17037_length_1794_cov_2.985624"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1794
                     /locus_tag="PK742_13565"
     CDS             18..>1794
                     /locus_tag="PK742_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77667.1"
                     /translation="MRTSNWLLGLIILLSLPASAQVLNAWDVNTLTGYGPSPYGPTTE
                     AANITSSGWTRAAGVATSGTAANYAWGGTGFALTTDAATAIAGNDYVFFTITANSGYT
                     VSFSEIPTFRYRHSGSGPDSALLQYSVGGGSYSDIVGFSFPSTSSSGANAIPLPVDLS
                     TITDLQAVPAGTTITFRLVLLGATNSGGTWYIFNQTGDDFVINGFTTPDGCTTPDDVP
                     SITATATGSTTATLNWTSATCYDEYLIVAAEGGSVSAGSPSGDGTAYTADADFSGSGS
                     ALLGGKVVYKGTALTADITGLSPGTDYCFKIFTRKGTNWSAGVEDCITTLNPTVYNPG
                     EMVFVGYDTNLGSGTDKFSIAVMEDMTPGTSFILANMVYEYRAAAYESTGLWYNGNGD
                     LTNSPGFVEITYIGSTVINAGSVICVELSDASANIVNAVFVDGTDASSDFIDDGGGHV
                     NVSSTAGNPDAMWLMQGTFSDTFFDSGDSVRTFVGSVLGGIQFRSSFQPFSADGDAGG
                     SRVSRIHPDIRCIGIDITTDASAFYGYYNSGAPHTGSARYILGNIIDVASNWTISTGG
                     ASTDELGTTCSTTFTITGSLEPGIWL"
BASE COUNT          426 a          459 c          460 g          449 t
ORIGIN      
        1 tgcagtgaac ctggtttatg cgtacctcca actggctttt gggcctcatt attttattgt
       61 ctctgccagc ctcggcacag gtattgaatg cctgggatgt gaatacactg accggctacg
      121 gaccctctcc gtatggccct accacggaag ctgccaacat caccagcagc ggctggacca
      181 gggccgccgg ggtggctaca tcaggtacag cggcaaacta tgcctggggg ggaaccggtt
      241 tcgcactcac caccgacgct gccactgcta tagctggcaa cgactacgtc ttttttacaa
      301 ttactgccaa ttcaggatac accgtttcct ttagcgagat cccgaccttc agatacagac
      361 attcaggctc aggccctgat tctgcattgc tccaatacag cgtcggtgga ggatcttatt
      421 cagatattgt tggtttcagt ttcccatcaa cctcttcttc aggagcaaat gctattcccc
      481 tacctgtaga cctatcgact attacagatt tgcaagctgt acctgcaggt actacaataa
      541 ctttcagatt ggtattactg ggagcgacca attcaggtgg tacctggtac attttcaatc
      601 agaccggcga tgatttcgtc atcaacggat tcaccacacc tgacggctgt accactccgg
      661 atgatgtgcc gtctatcaca gccacagcaa ccggatcgac caccgccacc ctgaactgga
      721 cctctgccac ctgttacgat gaatacctga tcgtagctgc agaagggggt tctgtcagtg
      781 ccggaagccc ttcgggcgat ggaacagcct acacagctga tgcagatttt tccggaagcg
      841 gctcagcgtt gctgggcgga aaggtggtct ataaaggaac cgcccttact gccgatatca
      901 ccggcctgag tccgggcacc gattactgtt tcaagatatt cacccggaag ggtacgaact
      961 ggtcggccgg cgtggaagac tgcatcacta ccctgaaccc aacggtgtac aatcccggcg
     1021 aaatggtttt cgttgggtat gataccaacc tgggaagcgg taccgataaa ttcagcattg
     1081 cggtcatgga agacatgacc cccggcacca gtttcatcct ggccaacatg gtatatgaat
     1141 accgggctgc agcctatgaa tctaccggac tgtggtataa tggaaacggc gacctgacca
     1201 attcgcctgg atttgttgaa ataacttata taggaagtac tgttattaat gcaggttctg
     1261 tcatttgtgt agagctgagt gatgcttctg caaatattgt taatgcagta tttgtggatg
     1321 gtactgatgc aagcagtgat tttattgatg acggaggtgg ccatgtcaat gtgagcagta
     1381 cagcagggaa ccccgatgcc atgtggctga tgcagggtac tttttcagat actttttttg
     1441 actcgggcga tagtgtaaga acatttgttg gaagtgtgct gggtggtatc cagttcagaa
     1501 gcagcttcca gcccttcagt gcagatggtg atgccggtgg ttcacgtgtg tccaggatcc
     1561 atccggacat cagatgcatt ggcatagaca tcactacaga tgccagtgca ttctacggat
     1621 actacaacag cggtgctcct cataccggtt cggcccggta catcctgggc aatatcatag
     1681 atgtggcatc caactggacc atttcaacag gcggtgcgag tactgatgag ctgggcacca
     1741 catgcagtac cacattcacc atcacaggaa gcctggaacc gggtatctgg ctgg
//
LOCUS       DAOMAX010000428         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_17146_length_1788_cov_3.186382, whole genome shotgun sequence.
ACCESSION   DAOMAX010000428 DAOMAX010000000
VERSION     DAOMAX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17146_length_1788_cov_3.186382"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK742_13570"
     CDS             <1..202
                     /locus_tag="PK742_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77668.1"
                     /translation="ISVTNDFVITLEWIQELPDPMESFLFCAGFLSEGLVYRKTSQDV
                     FTKFNIAGIGMYCEAEMEKPLK"
     gene            228..530
                     /locus_tag="PK742_13575"
     CDS             228..530
                     /locus_tag="PK742_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77669.1"
                     /translation="MKRFLFPVVWTILLFISGKDYLHAGHDHLCNHGQHEQNSSVLVN
                     SYICFACTVVLTTGEIARQPDAVPAVIITPAVRIGQEIVSITPTTCTVLLRGPPVV"
     gene            562..>1788
                     /locus_tag="PK742_13580"
     CDS             562..>1788
                     /locus_tag="PK742_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU77670.1"
                     /translation="MKYLFFILWVLPVCVQAQHDSCRLKLSGYVLDKQSHEALAFASV
                     YLSQSETGTITDLDGHFELNDLCPGDADLVISHLGCQSRTFRVQLTGDTAYTFHLEHD
                     SRELNEVVVTEHREAYTGSLPTSSISSVRLQAISGKPLGTMLKEVPGVWTLQTGPSIA
                     KPMIHGLHSNRVLIVQDGVRQEGQQWGSEHAPEIDPYTAGEITLIQGAAGVRYGSDAI
                     GGVILVQPPPYPTDTAVHGSISLAAFSNSLGGSGATRWSGQSKKLPAMQWRATGSYRL
                     GGDAATPNYRLANTAFRENNLSGAVKWSKAAWQTELGYSRFSTDLGILSASHIGNLTD
                     LEIALQADTPLILRDFSYYIQAPYQHIVHQVIRSFSTFRTGDIGHLDLNIAYQDNHRQ
                     EYDLHRMSDGGPALDLS"
BASE COUNT          429 a          481 c          458 g          420 t
ORIGIN      
        1 catctctgtg accaacgatt ttgtcatcac cctggaatgg atacaggaac tgcctgatcc
       61 catggagagt tttctgtttt gtgcaggttt tctgagcgag ggtctcgtgt accggaaaac
      121 cagccaggat gtcttcacaa aatttaacat cgccggcata ggaatgtact gcgaagccga
      181 gatggaaaaa ccacttaagt gattttttgc cctatctttg ttatggtatg aaacgcttcc
      241 tgtttccggt agtctggacg atcctgctgt tcatcagcgg caaagactat ctgcatgcag
      301 gtcatgacca cctctgcaat catggtcagc atgaacagaa cagcagcgtc ctggttaaca
      361 gctacatatg ttttgcctgt acggtcgtgc tgaccacggg tgaaattgcc agacaacctg
      421 atgctgttcc tgctgtgatc attaccccgg cagtccggat tggccaggag atcgtatcta
      481 tcactcccac cacctgtacg gtattgttaa gaggcccgcc tgttgtctga cagcctggtt
      541 ttctgacata caagtacata tatgaaatat ttatttttta ttttatgggt cttgcctgta
      601 tgcgtgcagg cgcaacatga cagctgccgt ctgaaactgt ccggctatgt gctggataag
      661 caatcacacg aagcgctggc atttgcttcg gtatatctgt cgcaaagcga gacaggtacc
      721 atcacagatc tggacggaca ttttgaactg aacgacctct gtccgggaga tgctgatctg
      781 gtcatctccc atctgggatg ccagtcacgt acctttcgtg tgcagcttac cggagataca
      841 gcctacacct tccacctgga acatgacagt cgcgaactga atgaagtggt ggtaaccgag
      901 caccgggaag cctataccgg aagtttgcct acttcatcaa tcagcagtgt caggctgcag
      961 gccatcagcg gaaaaccatt gggaaccatg cttaaggaag tgccgggtgt ctggaccctg
     1021 caaacagggc cttccattgc caaacccatg atacacggac tgcacagcaa ccgggtactt
     1081 attgtccagg atggtgtcag gcaggaaggt cagcagtggg gttcagaaca tgctccggag
     1141 atcgatcctt acacagccgg cgagatcacc ctgatacagg gagcagcagg tgtgcgctac
     1201 ggcagcgatg ccattggtgg tgtcatcctg gtgcagcctc ctccctaccc caccgatact
     1261 gccgtgcacg gatccattag cctggcagct ttcagcaaca gcctgggggg cagtggtgcc
     1321 acacgatgga gcggacagtc gaaaaaactc cctgccatgc aatggcgtgc cacaggaagt
     1381 tatcgcctgg gcggagatgc agctacaccg aattaccggc tggctaatac agcgttcaga
     1441 gaaaacaacc tgagcggtgc tgtgaaatgg tcaaaggctg cctggcaaac agagttaggc
     1501 tacagtcgtt tcagcactga cctgggcatt ctgtcggctt cacacatcgg taacctgacc
     1561 gatctggaga tcgccctgca ggcagataca ccactcatcc tgcgtgattt ttcctattac
     1621 atccaggcac cctatcagca tatcgtacac caggtgatcc ggtccttttc cactttcagg
     1681 accggcgata tcggtcacct cgacctgaac atcgcctacc aggacaatca ccgacaggaa
     1741 tacgacctgc accggatgtc tgacggcgga ccggcactgg acctcagc
//
LOCUS       DAOMAX010000429         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_17449_length_1771_cov_2.685897, whole genome shotgun sequence.
ACCESSION   DAOMAX010000429 DAOMAX010000000
VERSION     DAOMAX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_1771_cov_2.685897"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PK742_13585"
     CDS             complement(<1..619)
                     /locus_tag="PK742_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77671.1"
                     /translation="MKHITLISLLLLTQSVHSQTDSLPLFRYHLSGYLKALPSLTFDH
                     IADDTYGLYIIHNRINQKLEIGEQTTVRLEMRNRLFTGDYISLIPGYADLLETDKGLV
                     DLTFNWLEEDELIGNSTFDRAQVDWYGGKWEVTLGRQRINWGVHTVWNPNDWFNSFNF
                     LDFDYVERPGSDAVRVQYNLGNSRKLELAVAPGKLANDDVVALRYG"
     gene            complement(949..1656)
                     /locus_tag="PK742_13590"
     CDS             complement(949..1656)
                     /locus_tag="PK742_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU77672.1"
                     /translation="MARTVIDAHELSKTYTEGPVPVEAVKDVHLHIEAGEFIALVGPS
                     GSGKTTLLNMIGGLDEPTKGKVVIDGTDITGMKENKLIDFRLRNIGFVFQAYNLIPVL
                     TAEENIEFIMLLQGTDKDERKQRVQQLLEEVGLQGKGSSRPSELSGGQQQRVAVARAL
                     ASKPKFVLADEPTANLDSTTAANLLDIMEKLNKEEGMTFVFSTHDPRVIQRARRVVTL
                     VDGEIEKDELVNEPMVD"
BASE COUNT          326 a          465 c          501 g          479 t
ORIGIN      
        1 agccatagcg cagggccact acatcatcat tagccagctt gccgggtgcc acggccagtt
       61 ccagcttgcg gctgttgccg aggttgtact ggaccctaac agcatcggag ccgggacgtt
      121 ccacatagtc gaagtcgagg aagttgaagc tgttgaacca gtcgttcggg ttccagacgg
      181 tgtgcacccc ccagttgatg cgctgccggc caagtgtcac ctcccacttg ccgccgtacc
      241 agtccacctg tgcacgatcg aaagtggagt tgccgatcag ctcatcctct tccagccagt
      301 tgaaggtgag gtccaccagt cccttgtcgg tttcgaggag atctgcgtaa ccgggaatga
      361 gggaaatgta atcgccggtg aagaggcggt tgcgcatctc gagtctgacg gtcgtctgct
      421 ctccgatctc cagcttctgg ttgatgcggt tgtggatgat gtagagcccg taggtatcgt
      481 cggctatgtg atcgaaggtg agggagggta aagccttgag gtaaccggag aggtggtaac
      541 gaaacagagg cagactgtct gtctgtgagt gaacagattg cgtgaggagg aggagtgata
      601 tgagggtgat gtgtttcaat ttataggatc atgttcattc ttctctctag gagattccct
      661 gcctgacgga aggcaggcgg ctcgggggac ggtgcttcgt tgaggctgcg gttcaggttt
      721 tgcctttgtg ccctttgcgt atccattgcg tctttgtgtt aatgccttta ttggctgtgc
      781 tgttttgatt ccggaaactg agttgttatt cattggctga atgctcctct ttcattcttt
      841 tagatcccct gcctgacgga aggcaggcgg ctcggaggcc gggatgacac cgtttttttt
      901 atgctttgtc tttcatccgg tgttctccga agttcatcgg ctaataggct aatcaaccat
      961 cggctcatta accaactcgt ctttctctat ctctccatcc accagcgtca ccactctcct
     1021 tgcccgctgg atgacccgcg ggtcgtgggt ggagaagacg aaggtcatgc cttcctcttt
     1081 gttgagtttt tccatgatgt cgagcaggtt ggcggcggtg gtactgtcga ggttcgcggt
     1141 gggttcatcg gccaggacga atttcggttt gctggccagc gcccttgcca cggccacccg
     1201 ctgttgttgt ccgccgctca gctcgctggg tcgggaactc cccttcccct gcaggcccac
     1261 ttcctccagc aattgctgca cccgctgctt cctttcatcc ttgtccgttc cctgcagcag
     1321 catgatgaac tcgatgttct cttctgctgt gagcactggt atcaggttgt aggcctggaa
     1381 gacgaaaccg atgttccgca agcggaaatc aatgagcttg ttctctttca tgccggtgat
     1441 gtcggtacca tctatcacca ccttcccttt ggtgggttca tccagtccgc cgatcatgtt
     1501 cagcaaggtg gtctttcccg aaccggaggg acctaccagg gctatgaact cgccggcttc
     1561 gatatgtaag tgcacatctt tgacagcttc caccggcaca gggccttcgg tataggtctt
     1621 gctgagttcg tgtgcatcaa tgactgttct ggccataact tatatttttc tgatcgcttc
     1681 cacaggtttg agtttcaatg cccgcatggc cgggaaaacg gaggcgatca gcgccgctgc
     1741 cacgaccatg atgagtatgc tgaaatagga g
//
LOCUS       DAOMAX010000430         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_17517_length_1767_cov_4.268107, whole genome shotgun sequence.
ACCESSION   DAOMAX010000430 DAOMAX010000000
VERSION     DAOMAX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17517_length_1767_cov_4.268107"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="PK742_13595"
     CDS             complement(<1..1614)
                     /locus_tag="PK742_13595"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQU77673.1"
                     /translation="MHRLTHLPAVRLLLLLLPGMLLGIHMLSLPVGWWYINAFVFLLL
                     LLSGSVRSIRFRTALAMIQGISMASLIFLLAVTYGHFRTPWNISDHYVHFSELSGERV
                     FRIEEILKPTQRGVQALASVEALHTEEHRIMCSGNILIYLDTSGLRQSIQPGERFLTS
                     RMPVDPSGPKNPGAFDYRRYLETQGIKQVWYLRGDDLKRLQYDPPGMLRRWTLASRDY
                     ARAALSTALPDSTVRGVALALIIGDRSLLDAGVRDTFSGTGTMHILAVSGLHVGILYV
                     ILEKMLLLFPLFRRRDILKGYAAPVVIVCFIWFYALLTGLSPSIFRSAIMFTFLAFGR
                     PAGRYVNSFNILAASAITLLLIDPMMIRQVGFQLSFLAVWGIISFQPLLAGLWYPQSR
                     ILQYFWSLLTVSVGAQLGTAPISIYYFHIFPNYFLLANMVAIPLSFLILSNGMFTIVT
                     AFLPAVSSTSGILLDHLLRSLLWCLEQLSSVPGALTAGLYPLFQVTILLYLLLWSGWL
                     YLEHRKFFLLAVVICLAVLVTTLQELYIWR"
BASE COUNT          525 a          413 c          464 g          365 t
ORIGIN      
        1 acgccatata tagagctcct gcaatgtggt gacaagcacg gccagacaga tgaccactgc
       61 aagaagaaaa aatttgcggt gttccaggta cagccatccg gaccacagca gcaggtataa
      121 caggatcgtt acctggaaca agggatacaa tccggctgtc agggcacccg gaacagatga
      181 cagttgttcc aggcaccaga gcaaggatcg taacagatga tccagcagga tacccgatgt
      241 agaagaaaca gcaggcagga aagccgtaac aatggtaaac atcccgttac tgagaatgag
      301 aaaggaaagt ggaatagcca ccatattggc cagaaggaag taattcggaa atatgtgaaa
      361 atagtatatg gaaataggtg ctgtacccag ctgtgcaccc actgaaacgg ttagcagtga
      421 ccagaaatat tgtaatatcc tgctttgtgg ataccaaagt cctgccagta gaggctggaa
      481 gctgataatg ccccagactg ccagaaaaga aagctgaaag cctacctgcc tgatcatcat
      541 cggatctatc agcaacaggg taatggctga tgctgcaagt atgttgaagg aattgacata
      601 cctgcccgcc ggccggccga aggcgaggaa agtgaacatg atcgccgacc tgaatatgga
      661 tggcgacaag cccgttagca gggcataaaa ccagataaaa caaacgatca ccacaggtgc
      721 tgcatagcct ttcaaaatat ctcttctcct gaaaagtgga aaaagcagca gcattttttc
      781 cagtatcaca tatagaatac ctacatgtaa tccgctcact gcaagtatgt gcatagttcc
      841 ggttcctgaa aatgtgtctc tgacccctgc gtccaaaaga gatctgtcgc caatgatgag
      901 cgccagtgcc acacctctca cagtgctgtc cggtaatgcc gtgcttaaag ctgcacgggc
      961 gtagtcacgt gaggccaggg tccacctgcg cagcattccc ggcgggtcat attgcagcct
     1021 tttcagatca tcacccctca gataccagac ctgctttatg ccctgtgttt ccagataccg
     1081 gcgatagtca aatgcacccg gattctttgg tccggacgga tcaacaggca tccggctggt
     1141 cagaaaacgc tctcccggct gaatgctctg cctgagtcct gatgtgtcca gatagatcag
     1201 gatattacct gagcacatga tccggtgttc ctccgtatgg agtgcttcta cagaagcaag
     1261 agcttgcaca cctctctgtg tgggtttaag gatttcttct atccggaaga ccctctcgcc
     1321 tgataattcg ctgaagtgta catagtgatc tgaaatattc cagggagttc tgaaatgacc
     1381 gtaagtaacc gccagcagga agatcagact cgccatcgaa ataccctgta tcatggcaag
     1441 tgctgttctg aaacggatgg aacgaaccga tccggatagc agtagcaaca ggaaaacaaa
     1501 agcattgata taccaccatc caacaggcag agagagcata tggataccca gcagcatacc
     1561 aggcaaaagc agcagcagca ggcgaactgc cggcagatgg gttaagcgat gcatgaagga
     1621 ggtaaatata tggaaaaagt ggaaagacag gaatgcggag tggggagtgg ggtgtggggt
     1681 gtggggtgtg aggagtgggg agtggagagt gagggaaatg atctcacatt tccatttgcc
     1741 cgtatcgaac gacgaaaagc gatccac
//
LOCUS       DAOMAX010000431         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_17896_length_1746_cov_4.008870, whole genome shotgun sequence.
ACCESSION   DAOMAX010000431 DAOMAX010000000
VERSION     DAOMAX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17896_length_1746_cov_4.008870"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1746)
                     /locus_tag="PK742_13600"
     CDS             complement(<1..>1746)
                     /locus_tag="PK742_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQU77674.1"
                     /translation="PEAIHQIRRMAVSAGLLLKFFEGKKELYPTLAAFTEHIVYDKRI
                     QQLVNEVLDDDGNMRPDASPELAQIRKGMEQKSRELDKAFNAALRKMRSEGKLGDVEE
                     SMRNNRRVLGIAAEYKRQVKGIIHDESETGKTTFIEPEEVVWIQNELFELERAEQREI
                     YRLLKELCRELSPYREQLEQHQWLLSIMDVNRAKAKYALEISAASPRIDDKPIVHLYH
                     ARHPVLEQHYRPMKKPVIPMDIELNDDHRIVVISGPNAGGKSVSMKTVGLLVMMAQSG
                     LPIPAGDQSRVGIFQQLMADIGDSQSMEDELSTYSSRLMKMRQFIQKGNGKTLLLIDE
                     FGTGTDPAMGGAIAQAALEALNEKKVFGVITTHYANLKSYAENHPGIINASMVYDEAE
                     LRPTYQLEMGKPGSSYAMEIAQKSKLPQTTIDLARSLMSKEHVRFEELLKSVRVEKEF
                     LKLREKEFKQQQADLQKREQELQRRIRKNQEKEAEFQRRRLDKQQEALRKMEAEFREH
                     MQALKDAQPADMQAAREKLRKFIADNKKDQLQQAKKIRGPRAKASDPDALKEGSVVML
                     LDGEERGTIESIRND"
BASE COUNT          289 a          511 c          452 g          494 t
ORIGIN      
        1 tgtcgttgcg gatgctttca atggtgcctc gttcttctcc gtccagcagc atgaccacag
       61 agccttcttt cagggcatcc gggtcgctgg ctttggcccg gggaccgcgg atcttcttgg
      121 cctgctgcag ctggtctttc ttattgtcgg caatgaactt gcgcagcttc tcacgggcgg
      181 cctgcatgtc ggcgggctga gcgtctttca atgcctgcat atgctcacgg aactcggctt
      241 ccattttgcg caaggcttcc tgttgtttgt ccaggcgcct gcgctggaac tcggcttctt
      301 tctcctggtt cttgcggatg cgtcgctgca gctcctgttc ccgtttctgc agatcggcct
      361 gctgctgttt gaattccttt tcccgcagct tgagaaattc cttttctacc cgcacactct
      421 tgagcagctc ttcaaagcgc acatgctcct tgctcatcag cgaacgggcg aggtctatcg
      481 tggtctgtgg cagtttgctt ttctgggcga tctccatcgc atagctgctg ccgggcttgc
      541 ccatctctaa ctggtaggtg ggacgcaact cagcttcatc atacaccatg ctggcgttga
      601 tgataccggg atggttctcg gcatagctct tcaggttggc atagtgtgtg gtgatgaccc
      661 cgaatacctt cttctcgttc agtgcctcta atgctgcctg ggcaatggcg cctcccatag
      721 cgggatcggt accggtgccg aattcatcaa tgagcaacag ggtcttgccg tttcctttct
      781 ggatgaattg ccgcatcttc atcaaccggc tgctgtaagt actcagctcg tcttccatgc
      841 tctggctgtc tccgatatcg gccatcagct gctggaagat gcccacacga ctctgatcgc
      901 ccgcgggaat cggcaatccg ctttgagcca tcatgaccag caacccaacg gtcttcatgg
      961 agacggattt acctcctgcg ttcggtccgc tgataaccac tatcctgtga tcatcattca
     1021 gctcgatgtc catcggaatg acgggcttct tcatcggacg ataatgctgt tccagcaccg
     1081 ggtgacgggc atggtataga tggactatag gcttatcatc aatgcggggt gatgcggcac
     1141 ttatctccag tgcatacttc gccttggccc gattcacatc catgatgctg agcagccact
     1201 ggtgttgttc cagttgttcg cggtaggggg agagttcgcg gcatagttct ttcagcaggc
     1261 gatagatctc ccgctgttct gcccgctcca gttcaaagag ttcgttctgt atccacacca
     1321 cctcttcggg ttcgatgaag gtggtcttgc cggtctcgct ttcgtcgtgg atgatgccct
     1381 tcacttgccg cttgtattcg gcagcgatac ccagcacccg ccggttgttg cgcatgcttt
     1441 cttccacatc gcccagcttg ccttcgctgc gcatcttccg caaggcagca ttaaatgcct
     1501 tatccagttc ccggctcttc tgctccatgc cctttctgat ctgcgccagt tcaggcgatg
     1561 catcgggacg catattgcca tcatcgtcca gcacttcatt caccagttgc tggatgcgtt
     1621 tgtcgtagac aatgtgttct gtgaatgctg ccagggtagg gtagagttcc ttctttccct
     1681 caaagaattt cagcaacagt ccggcactca ccgccatgcg tctgatctgg tggatggctt
     1741 ccggcg
//
LOCUS       DAOMAX010000432         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_18086_length_1737_cov_4.114744, whole genome shotgun sequence.
ACCESSION   DAOMAX010000432 DAOMAX010000000
VERSION     DAOMAX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18086_length_1737_cov_4.114744"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PK742_13605"
     CDS             <1..342
                     /locus_tag="PK742_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77675.1"
                     /translation="NDQKDLQIIDSLNKEIVSHYGCSLEFSLGILNGKGWYFYGEKEY
                     DKAIEAWRLLLNSYPNLSEGYLYIIDAQVQLQQDASKTIEEFNRSLARSEIYTDQEKK
                     ELIQELENLIK"
     gene            656..1102
                     /locus_tag="PK742_13610"
     CDS             656..1102
                     /locus_tag="PK742_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77676.1"
                     /translation="MKDHEYPSSDVLSLDQITDRDTISALSPEQRSAFEALNTLQVST
                     DQIHHLYSTFAGINQPCYPPDTSFVLSQSDLLIALKHFVNIHCTNLNITKRNELASSA
                     VLAQKEYTVFPCLDRSASISYANGVPMTGTWVLPNILGRRDVIIVW"
     gene            1295..>1737
                     /locus_tag="PK742_13615"
     CDS             1295..>1737
                     /locus_tag="PK742_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77677.1"
                     /translation="MKKNTLLLLILMSCCQLQAQSRISAADFSILNNTSWQGTLTYID
                     GQTGNPTDIATTMQIKTDKNIIIQDIQYVWEPDKNVHASTRIRKGGRFLGKQKVISIN
                     YQDGDTIEIVTSASGKEHGTKAEFQYTYNFGPDHYVVTKNARLEGS"
BASE COUNT          556 a          374 c          328 g          479 t
ORIGIN      
        1 aatgaccaga aagatctgca aatcatagat tccctgaaca aagaaattgt tagtcattat
       61 ggttgctcgc tggaattcag cctgggaatt ctgaatggaa agggttggta tttttacggc
      121 gagaaagaat atgacaaggc catcgaagca tggagattac tactgaactc ttaccccaat
      181 ctttctgaag ggtatttata tatcatagac gcacaggtac agttacaaca ggatgcttcc
      241 aaaaccattg aagaattcaa ccgatcttta gcaagatcag agatctatac tgaccaggag
      301 aaaaaagaac tgatccagga acttgaaaat cttattaaat aacacaagca tgcttgtttt
      361 gctgaaatac atacatcatt taaaagtacc ttaaagtaag tatcctaaca ggttacagca
      421 aacatatgag ccccggtgtc agtatcgctg actcgcctac tcagaacttt tccgcttttg
      481 cgcagaaaga aaactttact acaaggccag cactcacatc ggcaaattat ttatggctat
      541 ataatcttat atcgttgccg gctaaagtaa catcactatg caatttgaat taaagccgca
      601 atttatggta ttgacagcag tcacctttct atcttcttgt gtatctgaca ataaaatgaa
      661 agatcatgaa tatccttcat ctgatgtgct gtcattggat cagattacag acagagatac
      721 catatctgca ctttctccgg aacaacgatc tgccttcgag gccttaaata ccttacaggt
      781 tagcacagac cagatccatc atttgtattc cacttttgcc ggtattaacc agccttgtta
      841 ccctcctgat accagctttg ttctttcgca atcagatctg ttgattgccc tgaagcattt
      901 cgtcaatata cattgcacca acctgaatat tacaaaacga aatgagcttg cttcctctgc
      961 tgtgttggct cagaaagaat acaccgtatt tccttgtctg gatcgttccg cttccatatc
     1021 gtatgcaaat ggtgttccaa tgactggtac gtgggtatta ccaaacatac tgggtcgcag
     1081 agatgtaata atagtatggt gaccccgccc caaaaaaaac caccggaaca aaacgggtac
     1141 aattttgttc atcagaattt ggagagattc agtttataat ctccggtctt ttgcctgatg
     1201 accttttttg ttgtatcatc ctgacagaag accagatatt cagtagggtg ttatttcatt
     1261 gaacagaaaa cctaacgaac caaaaagaat cagtatgaaa aaaaatacac tgctccttct
     1321 aattcttatg tcttgctgtc agcttcaagc acaatcccgg atctcagctg ctgatttcag
     1381 cattctcaac aatacaagct ggcagggtac actgacatac atagacggac aaacaggcaa
     1441 tccgactgac atagccacaa ccatgcagat caaaacagac aagaacataa ttattcagga
     1501 tattcaatac gtttgggagc ccgataaaaa tgtgcatgca agtacccgga tcagaaaagg
     1561 ggggcgattt ctcgggaaac aaaaggttat ctctattaat taccaggatg gagataccat
     1621 tgaaattgtt acttctgcat ctggcaaaga gcatggcacc aaagcagagt ttcaatatac
     1681 ttataacttt gggcctgacc actacgtggt aacaaaaaat gcccgtctgg agggctc
//
LOCUS       DAOMAX010000433         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_18122_length_1735_cov_3.126190, whole genome shotgun sequence.
ACCESSION   DAOMAX010000433 DAOMAX010000000
VERSION     DAOMAX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18122_length_1735_cov_3.126190"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PK742_13620"
     CDS             complement(<1..311)
                     /locus_tag="PK742_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQU77678.1"
                     /translation="MCVEPAGIYIIFLVVLFILAISDLIVGVSNDAVNFLNSAIGAKV
                     ASRRTILIVAGLGIALGATFSSGMMEVARKGIFNPQMFMFDEIMVIFVAVMLTDILLL
                     D"
     gene            452..>1735
                     /locus_tag="PK742_13625"
     CDS             452..>1735
                     /locus_tag="PK742_13625"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77679.1"
                     /translation="MKKFRMLVWLLVGTMPLFAQQQTDSGEPGKFQVQADRFADLSVL
                     RYEIPGWDNLSLDQKKLVYYLTEAGYSGRDIIYDQNCRYNLTIRHALEHIVANYTGDR
                     TSKDWAAFMTYTKRVWFSNGIHHHYSMDKIQPGFSRDYFRDLMMSSNTWVDPEITEVI
                     FDPNLVPKKVSLDPEKDLLLSSAMNFYDADITQEEAEAFYAQRINKGDKKPVSYGLNS
                     KLVRTENGLKEVLWTENGMYGAAIEQIIYWLGKAVEVAENQDQADALRLLIDYYRTGD
                     LKTWDAYNIAWTEATDGDIDYINSFIEVYNDPLGYRGSYESIVEINDFEASARMKVMA
                     DNAQWFEDNSPIMDAHKKKNVVGVSYKVVNVAGESGDASPSTPIGVNLPNANWIRADH
                     GSKSVSLGNIVEAYNSAGGSGLLEEFAYTKEELERA"
BASE COUNT          500 a          377 c          421 g          437 t
ORIGIN      
        1 aagtcaagca gcaggatatc tgtcagcatg actgctacga agatgaccat gatctcatcg
       61 aacatgaaca tttgcggatt gaagatgcct tttctggcca cttccatcat accactggaa
      121 aaagtggcac ccagtgctat gcccaggccg gctacgatca gaatggttct acgggaagct
      181 accttggcac cgatggctga gttcaggaaa ttgaccgcat cattgctaac tccgactatc
      241 aggtctgaaa tggcaagtat gaagaggact accaggaata tgatataaat acctgccggt
      301 tccacgcaca caaaggaaac aagtttgggt aattagcctg atgcctcaat cttaacttta
      361 tcttaccaaa ttgttaatcg gaagtagggt ccgccaccag cttattcctg cctgaatacg
      421 taactttgct cataaacatt aaaatctttt catgaaaaag ttccgaatgc tggtttggct
      481 gctggttggc accatgcccc tcttcgccca gcagcagacc gatagcggtg agcccggcaa
      541 atttcaggtg caggccgacc gttttgcaga cctttctgta cttcgatatg agatccccgg
      601 ttgggacaac ctgagtctgg atcagaaaaa actggtctat tatctcacag aggccggcta
      661 cagtggacgt gatatcattt atgatcagaa ctgtcgttac aatcttacca tccgtcatgc
      721 gctggagcac attgttgcca actatacagg cgatcgcacc agcaaagact gggctgcatt
      781 catgacctac accaaacggg tctggttcag taacgggatc caccatcatt acagtatgga
      841 caagatccag cctggatttt cccgcgatta tttcagagat ctcatgatgt catccaacac
      901 atgggttgat ccagagatca cagaggtcat attcgatccc aacctggtgc ctaagaaggt
      961 aagcctggat ccggaaaagg acctgctgtt atcatctgcc atgaattttt atgatgctga
     1021 tatcacccag gaggaagccg aagctttcta tgctcagcgt atcaataaag gagataagaa
     1081 acctgtcagt tacggattga acagcaagct cgtaagaact gaaaatggtc ttaaggaagt
     1141 tttgtggaca gaaaacggca tgtatggagc agccattgag cagatcattt actggttagg
     1201 taaagctgtg gaagtggcag agaatcagga ccaggcagat gcacttaggt tattgataga
     1261 ctactaccgt acaggtgatc tgaagacctg ggatgcatac aatattgcat ggactgaagc
     1321 caccgatggt gatatcgatt acatcaattc attcattgaa gtgtataatg atccgcttgg
     1381 ataccgtggt tcttatgagt ctatagtaga gatcaatgat tttgaggcaa gtgccagaat
     1441 gaaggtcatg gctgacaatg cccagtggtt tgaagataat agcccgatca tggatgctca
     1501 caaaaagaaa aatgtagtag gagtttcata taaggttgtg aatgttgcag gcgaatcagg
     1561 cgatgcctct ccttccacgc ctattggtgt gaacctgcca aatgccaact ggatacgtgc
     1621 agatcatggt tctaagagcg tcagtctggg taatattgta gaagcctaca acagtgcagg
     1681 gggttccgga ttgctggaag aatttgccta taccaaagaa gagttggaac gggca
//
LOCUS       DAOMAX010000434         1730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_18247_length_1730_cov_2.543881, whole genome shotgun sequence.
ACCESSION   DAOMAX010000434 DAOMAX010000000
VERSION     DAOMAX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18247_length_1730_cov_2.543881"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(772..>1730)
                     /locus_tag="PK742_13630"
     CDS             complement(772..>1730)
                     /locus_tag="PK742_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77680.1"
                     /translation="TENPTWFDITTGSTIGIPTAFSFTNDGDVLFVGTSSGRLYRYSG
                     LLEADWEYVDLDLDGEADDFDPAIAGITQYLYPELFGARITDVSCNGDDPDQVVLSLG
                     GYGVDDNVYYSDDAMSDDAATFVSIADDLPNVPVYSILISRSNPDIVLAGTEFGVWSY
                     SLSTGGVWNSENAVVGNVPIFDIKEDWIRTPGCNAIYIGTHGRGYWRSVSLNTGTGCD
                     FSLGAPDDAGPIQEEIIAGIVISPNPTDEYAEATMTLTEAANVNIGVLNMSGIRVMDL
                     GTVYYAAGVTRIPLNVKTLSAGTYLVVFTVDGVITSRKITVY"
BASE COUNT          479 a          444 c          376 g          431 t
ORIGIN      
        1 tccgcatcag caatggacag cagaacagca ctacctcatt catagtagaa taatagtaca
       61 agacctgtat tctgatcccg gccagtccac tggccgggat tttttttaag aggacgttcg
      121 ctggtggtcg tttccacggt ccttgttttc attaagtccc atacactgca tgaccggctg
      181 gttgcaggac gcctgtccaa tcgttcaata acagatgccg atagtccgtg tactgatgta
      241 atgaggtata acggactcca cagcaggcat gacatacaga gcagatggag ccaatgttat
      301 ccgtcagagt tgaatcggat gcattttcaa ttgaatgttg ctctctctta cccccaatag
      361 cggaagacct tcactccatt tctcccagcg ggaacttaca gatacaaggg agagatgata
      421 accatacaac cagacacccg ctgatcacac agattcattg cagggcactc ctcagtcttt
      481 gtctgaatat gccatactgg tctttcccgg ttcggatata aagctgtttg acagcaacgg
      541 aagcaccacc tctcatcctg agttcgcaac cagtactcgt ggccagagct ccaggtttcc
      601 gttcacacct ggttaaaaca gttcatgctg cacaggtgat ccgcagacca gatactttga
      661 agctgagtaa acaactgtga tacattgata cccggtaact gttgttgtat agcgggcaca
      721 aaaaaaggct gccggaaaga ccggcagccc tggaaattaa taatatcagg atcagtacac
      781 ggtgatcttc ctgctggtga tcactccatc tactgtgaat actaccaggt aagtacccgc
      841 tgagagtgtt ttcacattca gagggattct ggttacacca gctgcataat aaacagtgcc
      901 taaatccatc accctgattc cactcatgtt cagcactcca atatttacat tggctgcctc
      961 tgtcagggtc atagtggctt ctgcatactc atctgtcggg tttggagata tcacgatacc
     1021 ggcaatgatc tcttcctgga taggacctgc atcgtccggt gcacccaggc tgaaatcgca
     1081 acctgtgcct gtattcaggg aaacgctcct ccaatagcca cggccatgtg taccaatgta
     1141 aatggcatta catccggggg tacggatcca gtcttccttg atatcaaaga tcggcacatt
     1201 accaacaaca gcgttttctg agttccatac accaccagta ctgaggctgt atgaccagac
     1261 tccgaattct gttccagcca gaacgatatc aggatttgac ctgcttatca ggatgctgta
     1321 aaccggaaca tttggcagat catctgcaat gctaacaaag gtggctgcgt catcagacat
     1381 tgcgtcatca gaatagtaca cattgtcatc aacaccataa ccgcctaaag acaatacaac
     1441 ctgatctgga tcatcaccat tacaggaaac atcagtgatc ctggcaccaa aaagctcagg
     1501 atacaggtat tgggtgatac cagcaatagc agggtcaaaa tcatctgctt caccatcaag
     1561 atcaagatca acatactccc agtcagcttc cagcaatccg ctgtagcggt agagccttcc
     1621 ggaagatgta cctacaaaca ggacatcacc atcgttggta aagctgaacg ccgtagggat
     1681 accgatggtt gaaccggtag ttatatcaaa ccatgttggg ttctctgttg
//
LOCUS       DAOMAX010000435         1721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_18397_length_1721_cov_4.053421, whole genome shotgun sequence.
ACCESSION   DAOMAX010000435 DAOMAX010000000
VERSION     DAOMAX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18397_length_1721_cov_4.053421"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PK742_13635"
     CDS             complement(<1..669)
                     /locus_tag="PK742_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQU77681.1"
                     /translation="MRKSTIWGFTILASVSLFFSSCFKDLDLSPKYGLNTESVYEDPA
                     NYIHVLAKLYAGLSITGNQGPAGSPDISGIDEGFSAYVRVLWNLQELPTDEAICGWND
                     PGIPELNSGTWNSTSSFVQAMYYRIFYQIPLCNEFIRFCSDDWMNEKGFSDADKATIR
                     TYKAEARFLRALSYYHAMDLFGNVPFVTEDDLPGAGFPEQIQRADLFDYIEDELLAIE
                     GERDK"
     gene            complement(682..>1721)
                     /locus_tag="PK742_13640"
     CDS             complement(682..>1721)
                     /locus_tag="PK742_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU77682.1"
                     /translation="SEGTSTAQYLFGDTYYYVLRPDGYDPNIKWEETMSYNVGLDFGF
                     HNNRWNGSLDFYKKITTDLLSVIPIPAGTNFTNQLLTNVGSMENTGAELTLNYVAIDN
                     ESTTLEFGGNVTVNKNEITKLNVLANDDDPGILVGGIAGGVGNTIQIQSVGFPTFTYF
                     TYEQVYAEDGSPIEGEYVDQNGDGVINPDDLIRFDGNPAPDMFLGFFANYTHNKWTAG
                     MSVRGEFGGYVYNNVNSSLGNYFNVGSTKGYLANMTTDYLNTVFQTPQYLSDYYVEKA
                     SYIRLDNIYVGYNFGRVIADKVGLALTAIVQNALVVSSYSGLDPEVAGGIDNNFYPRP
                     RTYGLNLNLQF"
BASE COUNT          404 a          415 c          448 g          454 t
ORIGIN      
        1 tttatctcgc tctccttcaa tggccagcag ttcgtcttcg atgtaatcaa agaggtcagc
       61 ccgctggatc tgttccggaa aaccggcacc cggcaggtca tcttcggtca cgaagggcac
      121 attgccgaac aggtccatcg catggtagta gctgagtgca cgcaggaaac gtgcttctgc
      181 cttgtaggtg cgtatggtgg ccttgtcagc gtcgctgaaa cctttttcat tcatccagtc
      241 atctgaacag aagcggatga attcattaca caatggtatc tggtagaata tcctgtagta
      301 catggcctgt acaaaggagc tggtggagtt ccaggtaccg gagttgagtt cgggaatacc
      361 cgggtcgttc cagccacaga tggcttcatc tgtgggcagt tcctgcaggt tccataatac
      421 ccgtacatat gcggagaatc cttcatcaat accactgata tctgggctgc ccgcaggccc
      481 ctggttaccg gtgatggaca ggcctgcata gagcttggcc agtacgtgga tataattggc
      541 ggggtcttca tagacagact ccgtgttcag tccgtatttc ggactcaggt ccagatcctt
      601 gaaacaggag ctgaagaaca gggatacgga ggccagaatg gtgaatcccc agatggtact
      661 ttttctcatc gtatctcgat tttagaattg aagattgagg ttcagaccgt aggtgcgtgg
      721 acgcggatag aagttgttgt ctataccgcc ggccacttcc ggatccagtc cggagtagct
      781 gctcaccacc agtgcattct gcacgatggc ggtcagggca agacctacct tgtcagctat
      841 cacccggccg aaattgtaac ctacatagat attatccaga cggatgtagg aagctttctc
      901 tacataatag tcagacagat attgtggtgt ctggaatacg gtgttcaggt agtctgtggt
      961 catgttggcc aggtatcctt tagtggatcc tacgttgaag tagttgccca gcgaagaatt
     1021 gacgttgtta tagacatagc cgccgaactc accacgtact gacatacctg cggtccattt
     1081 gttatgcgta tagttggcaa agaaacccag gaacatatcg ggagcagggt tgccgtcaaa
     1141 acgaatcagg tcatcagggt tgatgacacc atcgccgttc tggtcaacgt attctccttc
     1201 aataggtgaa ccatcttccg catacacctg ctcataggtg aagtaggtga aagtggggaa
     1261 gcccacactc tggatctgaa tagtgttacc tacaccaccg gcaataccgc ctaccaggat
     1321 acccgggtcg tcgtcattgg ccagcacatt cagtttggtg atctcgttct tgttgaccgt
     1381 tacgttacct ccaaactcca gggtagtgct ctcattgtca atagctacgt agttcagtgt
     1441 cagttctgca ccggtgttct ccatgctacc tacgttggtg agcagctggt tggtgaagtt
     1501 ggtaccggca ggtattggga tgacactcag cagatctgtt gtgatcttct tatagaagtc
     1561 caggctaccg ttccaacggt tgttatggaa accaaaatca agacccacat tatagctcat
     1621 ggtctcttcc catttgatgt tcggatcgta gccatcaggc ctgagcacat agtagtaggt
     1681 atctccgaaa aggtactggg ctgtgctggt gccttctgag t
//
LOCUS       DAOMAX010000436         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_18646_length_1709_cov_3.084039, whole genome shotgun sequence.
ACCESSION   DAOMAX010000436 DAOMAX010000000
VERSION     DAOMAX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18646_length_1709_cov_3.084039"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK742_13645"
     CDS             <1..419
                     /locus_tag="PK742_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQU77683.1"
                     /translation="AALPSGNLIVPVIKNADRMNLVGLTATVNDLASRARQNKLKPEE
                     IQGGTFTLTNVGSFGSIMGTPVINQPQVAILAVGAIKKRPMVLETPQGDVIGIRHMMY
                     MSMSYDHRVVDGMMGGSFLNRVAQLLEGFDPNRDVW"
     gene            complement(669..1043)
                     /locus_tag="PK742_13650"
     CDS             complement(669..1043)
                     /locus_tag="PK742_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77684.1"
                     /translation="MKTRTASLILMILTAAGILFHICILLKLIPYNIAWGGRLEDDAA
                     MYRMEIFSLVLNLFFAWMVAVQGGFIAGNKQRSRVVLWIFFVLFTLNTIGNVFAQTTT
                     EKLFAVLTLANVILLWFVLRKK"
     gene            complement(1040..1450)
                     /locus_tag="PK742_13655"
     CDS             complement(1040..1450)
                     /locus_tag="PK742_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77685.1"
                     /translation="MQRTTIYTKNLDEDSFRSACEDMLQILSDQGVLTLDLLFGWAWE
                     NRPANEQAHTTLLSNVMEEIEQATAAGQGRLGEDNVTLFIREHAVEIIWCHESDIHLG
                     WYESSTLVDAIRQHWKDTGIFRSEITNKPRRTDK"
     gene            complement(1452..>1709)
                     /locus_tag="PK742_13660"
     CDS             complement(1452..>1709)
                     /locus_tag="PK742_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQU77686.1"
                     /translation="VNFKPIEGKEAIRHKFREEFATAEMVCIIEQILECGEWGILEWK
                     DPLGLRGCGFFHIEDGKIRYQRGYWDMLSFLRQHKLPLPTE"
BASE COUNT          387 a          429 c          425 g          468 t
ORIGIN      
        1 cagcggcact gcccagcggc aacctcatcg tgcccgtcat caagaacgcc gaccgtatga
       61 acctggtggg actgaccgcg accgtgaatg acctcgctag cagagccaga caaaacaagc
      121 tgaagcccga agagatccag ggcggtactt tcacgctgac caatgtgggc agcttcggca
      181 gcatcatggg tacaccggtc atcaaccagc cacaggtagc tatcctggcg gtgggtgcca
      241 tcaagaagcg cccgatggtg ctggaaacac cgcagggcga tgtgatcggt atccgccata
      301 tgatgtacat gagcatgagc tatgaccacc gtgtggtgga cggcatgatg ggcggcagct
      361 tcctgaaccg tgtagcccag ttgctggaag gattcgatcc gaacagggac gtatggtgag
      421 aggagttgtt ggttattcgt tattcgtttt tggtggttgg ttattggttt ttagtcccga
      481 tagctatcgg ggcattcttc agtctatagt ctcgagtcgt cagtcttttt tttgacaacg
      541 atattatttc tttcaatccg tagagattct ttccctcctc tggcaggcag gggaatctgt
      601 ggtgtgttat tctattgagt tttttttcgc agttggtact ctcaccaacc gctttcgacg
      661 tcatctattt atttttttct caatacgaac cataacagta tcacattggc aagtgtcagc
      721 acagcaaaca gtttttctgt ggtggtctgt gcaaatacat tgcctatggt attcagggtg
      781 aacaggacga aaaagatcca gagcaccaca cgagagcgct gtttgtttcc tgcgataaat
      841 cctccctgca cggccaccat ccaggcaaag aacaggttaa gcaccagaga aaagatctcc
      901 atccggtaca tggctgcatc atcttccagt cgtccgcccc atgcaatgtt atacggaatc
      961 agcttcagca gtatgcagat gtgaaacagg ataccggcag cggtcagtat catcaggatg
     1021 agtgatgcgg ttctggtttt cacttgtcag tccttcgcgg tttgttggtg atctctgaac
     1081 ggaagatacc ggtatccttc cagtgttgcc tgatagcatc caccaatgta gaggactcat
     1141 accagccgag atgtatatca gactcgtggc accagatgat ctccactgca tgttctctta
     1201 taaacagggt gacattatcc tcacccagtc ttccctgtcc tgctgctgtt gcctgttcta
     1261 tttcttccat gacattggac agcaaggtgg tatgtgcttg ttcattggca gggcggtttt
     1321 cccaggccca tccaaacaag agatccaggg tcagtactcc ctggtcagag agtatctgca
     1381 acatgtcttc gcaagcgctt cgaaagctgt cttcgtccag gtttttcgtg tagatggtgg
     1441 tcctttgcat gttactcagt tggaagagga agcttatgct gtcgtaaaaa gctgagcata
     1501 tcccaatatc ctctctggta tctgatcttc ccatcctcga tgtgaaagaa gccgcatcct
     1561 ctcagtccca gcgggtcctt ccattcgagt atgccccact cgccgcattc caggatctgc
     1621 tcgatgatgc agaccatctc tgcggtggcg aattcttccc tgaatttatg acggatggct
     1681 tctttgcctt caataggttt gaaattcac
//
LOCUS       DAOMAX010000437         1697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_18880_length_1697_cov_3.049939, whole genome shotgun sequence.
ACCESSION   DAOMAX010000437 DAOMAX010000000
VERSION     DAOMAX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18880_length_1697_cov_3.049939"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..577)
                     /locus_tag="PK742_13665"
     CDS             complement(230..577)
                     /locus_tag="PK742_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HQU77687.1"
                     /translation="MNNLKNNQKSDAAPMGYDALLAPVNVRWIPLTEKEPEEGFEVLL
                     YNKNWINEDWNPKGVRVGYLDGVGGWISAYWCNYHDDYHTRTSDEDNKQFEDYKAENQ
                     IPTHWMEIPACGG"
     gene            complement(590..931)
                     /locus_tag="PK742_13670"
     CDS             complement(590..931)
                     /locus_tag="PK742_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77688.1"
                     /translation="MARGPIGHTCPDIDKTIGFLAQIRNEATYIQDHQPDCTESTYSA
                     ISTILDICNYVEASLEDLRSDNEQLRSWGADQEDDKNRAEDEAYRLHGQVSDLERRVA
                     ELEADVYRPLI"
     gene            complement(1059..1457)
                     /locus_tag="PK742_13675"
     CDS             complement(1059..1457)
                     /locus_tag="PK742_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77689.1"
                     /translation="MKEPRLSTAVINFLLTILICLFIILGSIKCHAQDSYLEQDDYYR
                     PDCYGKVLVSEYPDYKPVEFKYEVTLDEAVWTIAWEIPHGLQRKGWIWLKPPGWDEAI
                     VFDKWDRIPNEAYYFMLRYFPNEFTKLNLD"
     gene            complement(1454..>1697)
                     /locus_tag="PK742_13680"
     CDS             complement(1454..>1697)
                     /locus_tag="PK742_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77690.1"
                     /translation="MPEPYQNEILRDHAEQVERRTLNYKDELNNPVMSIGWNNMDSSL
                     RLKPQFLPMPIDAQMNVYLFNVDAQIKKLQGELAAL"
BASE COUNT          403 a          388 c          343 g          563 t
ORIGIN      
        1 aattaacgca cggtgcatcc ttgcatattt ttcatagaaa ttaccattta aaattaattc
       61 tccatttttt acatcctcat tcggaggaag aacaacgcct aacaatgtat aaccgtcaag
      121 cttggtttct gtttcttttg aaagtttttg ttcattcata atttctgtat tttaattaag
      181 ttagttcttt attcacgccc gaacggttat actttaccgt tagcaccaat tagccaccgc
      241 acgcaggaat ttccatccag tgagtaggta tttgattttc ggctttataa tcttcaaact
      301 gtttattatc ttcatcactt gttcgggtgt ggtaatcatc gtggtaattg caccaataag
      361 ccgaaatcca tccaccaaca ccgtctaagt aaccaactct tacccccttt ggattccaat
      421 cttcatttat ccaattttta ttatacaata gcacctcaaa accttcttct ggttcttttt
      481 cagttaacgg aatccaacgc acattaactg gtgctaacaa tgcatcatac cccattgggg
      541 ctgcatcgct tttttgattg ttttttaagt tattcatctg tatttttttt taaattaaag
      601 ggcggtacac atccgcttcc agttctgcaa cacgcctttc caggtctgat acctggccgt
      661 gcagtcggta tgcctcatct tcggcccggt ttttatcatc ctcttggtct gcgccccatg
      721 acctcagttg ttcattgtct gaacgtaggt cttccaggga tgcctctaca taattacaga
      781 tgtctaatat ggtgctgatg gcactatagg tgctttctgt gcaatccggc tgatggtcct
      841 gtatgtaggt agcctcattc cgtatttgtg ccagaaaacc aatcgttttg tctatatccg
      901 ggcaagtatg ccctatgggt ccacgggcca tcgttatgca gttttgggct tgaaaattgg
      961 tgcaggaaca aggctgtggt caatgtagtg gttccactgg tacgcctttt tacaagtgtg
     1021 gcagtagcat gttttgtgga gaattatcct tttctccatc agtccagatt taatttggtg
     1081 aactcattag gaaagtacct gagcatgaag taataggctt cattgggtat tctatcccat
     1141 ttgtcaaaga caatagcttc atcccatccc ggaggtttca gccatatcca gcctttcctc
     1201 tgcaggccgt gaggtatttc ccaggcgatt gtccagacgg cttcatcgag cgttacctca
     1261 tatttgaact ctactggctt gtagtcagga tattcgctta ccagcacctt gccatagcag
     1321 tcaggccggt agtagtcgtc ctgttccaga taggagtcct gtgcatggca cttgatggat
     1381 ccgagaatga taaacaggca gatcaggatg gtcagcagga aattgattac tgctgtggat
     1441 agtcttggct ctttcatagt gcagcaagct ccccctgcag ctttttgatt tgtgcgtcaa
     1501 cgttgaacag atacacattc atctgtgcat ctatgggcat aggaaggaat tgcggtttca
     1561 gacgcaagga tgaatccata ttattccagc caatgctcat caccgggtta ttgagttcat
     1621 ctttgtaatt caaagtccta cgctctacct gttcagcgtg atctctcaga atttcgtttt
     1681 gatacggttc cggcatt
//
LOCUS       DAOMAX010000438         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_19000_length_1691_cov_3.544621, whole genome shotgun sequence.
ACCESSION   DAOMAX010000438 DAOMAX010000000
VERSION     DAOMAX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19000_length_1691_cov_3.544621"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PK742_13685"
     CDS             complement(<1..338)
                     /locus_tag="PK742_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQU77691.1"
                     /translation="MEAHKPNTTGIAILYVEDDPSLAMVTADALSRKGYSVTHCSNGV
                     KAIEAFLKGDFQICIMDVLLPDIDGYSLAKVVRSTNESIPIIFLSGRNDDKNRLDGYR
                     AGGDDYITKP"
     gene            complement(510..1217)
                     /locus_tag="PK742_13690"
     CDS             complement(510..1217)
                     /locus_tag="PK742_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU77692.1"
                     /translation="MSSDKKYRILYVEDDEHLGFVTKDNLEKRGYEVIMCQDGLEAIQ
                     TFQQHRFDLCILDVMLPKVDGFTVAQKIRDKDTHVPVLFLTAKSLLEDKIQGFQLGGD
                     DYITKPFSIEELTYKIEVFIRRSLVLSENTSEKNIFEISKFSFDFKELSLTSGKEKHT
                     LTLREAELLRMFCLNLNQVVKRDDILKQVWGEDDYYIGRSLDVFVSRLRKYFSSDPSI
                     SIDNVRSVGFKLSVSGE"
     gene            complement(1207..>1691)
                     /locus_tag="PK742_13695"
     CDS             complement(1207..>1691)
                     /locus_tag="PK742_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQU77693.1"
                     /translation="RKEPINLRQLLEEIVPPFMVQLEAREGKFHKECPDTPCVVTADR
                     IHLKNVINTLLDNALKYSEGTPEIGIRIHCGEKNIYIQVIDNGIGIPKEYHKRIYEKF
                     FRVPTGDVHNVKGFGLGLSYVKMIAKAAGWKISLQSELNKGSTFSIGIPANTPMNDEQ
                     "
BASE COUNT          429 a          404 c          363 g          495 t
ORIGIN      
        1 taaggcttgg tgatataatc atcgccaccg gctctgtaac catcgagcct gtttttatca
       61 tcatttctgc ctgacagaaa aattataggt atgctttcat tggtagaacg aacaactttt
      121 gccaggctgt atccgtcaat atccggcaac agcacgtcca tgatacagat ctgaaagtct
      181 cctttcagaa atgcttcgat ggcctttact ccgttgctac aatgagtgac agaatatcct
      241 ttacgtgata aagcatcagc agttaccata gccaatgaag gatcatcttc tacatacaaa
      301 atggcaatgc cggtggtatt cggtttatgg gcttccacag ttttccttct ttaccggtac
      361 ctgaaccggt gatgctaagg tacgtgcagc accccctgat gaatgttaat gatccataag
      421 gcaatgcaaa aggattgtaa accaactgta agagtttttt aaaagaaaac aaaaaggaag
      481 agaatgcagt aatgccagag ttcaaaaatt cactctcctg acacactgag tttgaaacca
      541 acactgcgca cgttgtcgat gctgatactt ggatcactgc tgaaatattt gcgcaatctg
      601 gatacgaaaa catcaaggct tctgcctatg tagtaatcat cttcacccca gacctgtttg
      661 aggatatcat cacgtttgac cacctgattc agattcagac agaacatgcg gagcagttct
      721 gcttcccgca gggtcagggt atgcttttcc ttgccggagg tcaggctgag ctccttaaaa
      781 tcaaaagaga atttgctgat ctcgaagatg ttcttttcgc tggtattctc actcagcacc
      841 aggcttcttc tgatgaacac ttctattttg taggtgagtt cttctatact gaagggcttg
      901 gtgatatagt catcgcctcc cagctgaaag ccctggatct tatcttccag caatgatttg
      961 gctgtaagaa agagcaccgg cacatgggtg tctttgtcgc ggatcttctg tgctacggtg
     1021 aatccatcaa cctttggcag catgacatcc aatatacaca gatcgaaccg gtgttgctga
     1081 aaggtctgta tggcttccag accatcctga cacatgatca cctcatagcc ccgcttttcc
     1141 aggttgtctt tggttacgaa acccaggtgt tcgtcgtctt cgacatagag gatccgatac
     1201 ttcttgtcac tgctcatcat tcataggtgt atttgcagga atacctatac taaaggtact
     1261 gcctttgttt aattcacttt gcaggctgat cttccacccc gctgctttgg ctatcatctt
     1321 gacataactc aggcccaaac caaaaccttt gacattatgt acatcgccgg taggtacgcg
     1381 gaagaatttc tcatagatcc gcttgtggta ttccttggga atgcctatgc cattatcgat
     1441 cacctgtata tagatgtttt tttcgccgca atggatgcgt atgccaattt ccggggtgcc
     1501 ttcgctgtat ttcagggcat tatccagaag ggtattgatg acattcttca ggtgtatccg
     1561 gtcagcagtg accacacaag gtgtgtccgg gcactctttg tgaaattttc cttcccgggc
     1621 ctccagctgt accataaagg gaggtacgat ctcttccagc aattgccgca ggttgatcgg
     1681 ctccttcctc a
//
LOCUS       DAOMAX010000439         1686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_19096_length_1686_cov_3.532189, whole genome shotgun sequence.
ACCESSION   DAOMAX010000439 DAOMAX010000000
VERSION     DAOMAX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19096_length_1686_cov_3.532189"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>1686)
                     /gene="ggt"
                     /locus_tag="PK742_13700"
     CDS             complement(133..>1686)
                     /gene="ggt"
                     /locus_tag="PK742_13700"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQU77694.1"
                     /translation="GRQILEQGGNAVDAAIATQFALAVVFPVAGNIGGGGFMVVRLQN
                     GETATLDFREKAPSAATRNMYLDSTGQVDRNAITATQLASGVPGSVAGMWEAHQRYGS
                     LPWAMLLQPAVDLAAKGFPVTEAQAGMLNRAGEDFRERNTRNTYLRRYGDWHAGDTLK
                     QTDLAATLKRIQLEGRDGFYKGETARLIVEEMQAGNGIISYDDLANYDAVWRHPLQFR
                     YHEYTIISMPPPSSGGVALAQLLGMLEPFDLTALGHNTPDYIHLVAHAEQLVYADRAK
                     WLGDPDFYPVPQNRLTDTAYLHARMEGFDHTHATPSSDIAAGVPAGYESEQTTHFSIV
                     DAQGNAVSLTTTLNDGYGSKVFVTGAGFLLNNEMDDFSAKPGEPNLYGLIGGEANAIE
                     GNKRMLSSMTPSIVLQGDNLFMVTGTPGGSRIITSTMQSILNVTAFGMTMQESVSAPR
                     FHHQWLPDEIQYEREGLSTAIQASLRRLGYTLAPKAPFCRVDAILVIPDGRLEGGADP
                     RGDDTADGW"
BASE COUNT          366 a          448 c          451 g          421 t
ORIGIN      
        1 ttatttcaat attaatgaaa ataaaatgaa atgtcaagta gaaagtacaa agaattactg
       61 cctgacagaa ggtctgcatg aaattttata acagtaaata ggcctgtatt ccagagatct
      121 gaataaacaa tttcaccatc catctgccgt atcatcccct cgcgggtctg caccgccttc
      181 cagtctgcca tcaggtatga ccaggatggc atctacacga cagaagggtg ccttgggagc
      241 cagggtgtaa ccaagccttc tcaaggatgc ctggatggcg gtggacagtc cttcccgttc
      301 gtactggatc tcatcaggca accactggtg gtggaacctc ggcgcactga cactttcctg
      361 catggtcatg ccaaaagcgg taacgttgag gatgctctgc atggtagagg tgatgatgcg
      421 gctgcctcca ggcgtgccgg tcaccatgaa caggttgtcg ccttgtaata cgatgctggg
      481 ggtcatgctg ctcaacatgc gtttgttgcc ttcaatggca ttggcttcgc cgcctatcag
      541 tccgtacaga ttaggctctc cgggcttggc actgaaatcg tccatctcat tgttgagaag
      601 aaaacctgca ccggtcacga agaccttcga gccatatcca tcattcaaag ttgtggtcag
      661 actgactgca ttgccctgtg catccacgat gctgaagtgg gtggtctgtt cgctttcata
      721 acctgccggt acaccggcag caatgtctga tgatggtgtg gcgtgtgtgt gatcgaatcc
      781 ttccatcctg gcatgcagat atgcggtatc tgtcagccgg ttctgtggca ccggataaaa
      841 atcaggatca cccagccatt tggccctgtc ggcatagacc agttgttcag catgtgctac
      901 caggtggatg tagtctggtg tattatgtcc cagagcagtg agatcgaaag gttccagcat
      961 ccccagcagt tgtgcaagcg ccacgccacc actggatgga ggcggcatgg agatgatggt
     1021 gtattcatgg tagcggaact gcagcggatg ccgccacaca gcatcgtagt ttgccaggtc
     1081 atcataagaa atgatgccgt tacctgcctg catttcttcc acgatgagcc gggctgtttc
     1141 gcctttgtag aatccgtctc tgccttccag ctgaatgcgt ttcagcgtag ctgccaggtc
     1201 tgtttgtttt aatgtatctc ctgcatgcca gtcgccgtac cggcgcagat aagtgttgcg
     1261 tgtatttctt tcgcggaaat cttcgcctgc acgattgagc atacccgcct gtgcttcagt
     1321 aacgggaaat cctttcgccg ccaggtcaac agcaggttgc agcagcatgg cccagggaag
     1381 ggaaccatag cgctgatggg cttcccacat gccggctaca gaaccgggta caccagaggc
     1441 cagctgagtg gccgtgatgg catttctgtc cacctgtccg gtgctgtcca ggtacatatt
     1501 gcgtgtggct gcggatggtg ctttttcgcg gaagtcgagt gtggcggttt cgccgttttg
     1561 caatcgcacc accatgaaac ctccgcctcc gatattcccc gccacgggaa agaccactgc
     1621 cagtgcaaac tgcgtggcga tggctgcatc caccgcattg cctccctgct ccaggatctg
     1681 cctgcc
//
LOCUS       DAOMAX010000440         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_19309_length_1676_cov_4.252313, whole genome shotgun sequence.
ACCESSION   DAOMAX010000440 DAOMAX010000000
VERSION     DAOMAX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19309_length_1676_cov_4.252313"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /gene="cysS"
                     /locus_tag="PK742_13705"
     CDS             complement(<1..1181)
                     /gene="cysS"
                     /locus_tag="PK742_13705"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQU77695.1"
                     /translation="MPNELFHQNELLVYNTTTRSKEVFRPLHAPFVGMYVCGPTVYSE
                     PHLGNCRTFVSFDMIYRYLRHLGYKVRYVRNITDAGHIESTAGTTGDRISEQARLEQV
                     EPMEIVQKYSTAFRNIMAKFNALPPDIEPTATGHIIEQIEMTKAILESGYAYEVDGNV
                     FFDVEKFAKDHPYTQLSGRNLEEMLNNTRDLGGQELKKGRLDFALWIKAKPEHLQRWP
                     SPWGEGYPGWHIECSAMSTKYLGKQFDIHGGGMDLIPTHHTNEVAQSVACNHTTPVRY
                     WLHTNMLTVNGQKMSKSLGNSFLPEELFSGSHPLLDRGYSPMTVRFFMLQAHYSSTLD
                     FTNEALQAAEKGYRRLMSMLENLEALKPSGAVDDTTEKALNASMLAMYAGMNNDFNTP
                     QA"
     gene            complement(1205..>1676)
                     /locus_tag="PK742_13710"
     CDS             complement(1205..>1676)
                     /locus_tag="PK742_13710"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQU77696.1"
                     /translation="ADILIYGDTIRLFSTHLQSNQLTHSDFEFIDQTGSSDTLSFEPA
                     RVANKLRDSYKLRAEQVEVIRQALDASPHPVLFCGDFNDTPVSYAYRKISEGLDDAFL
                     HCGRGLGATYSPAPLIRIDYMLYTPAVLKALDYERRRSAGSDHYLVRSVFSLQP"
BASE COUNT          377 a          443 c          429 g          427 t
ORIGIN      
        1 gcctgcggag tattgaagtc attgttcatg cccgcataca tggccaacat agaagcattc
       61 aatgctttct ctgtggtatc gtctaccgca cctgatggtt tcagtgcttc caggttctcc
      121 agcatggaca tcagcctgcg ataccctttt tccgccgcct ggagcgcttc attggtgaag
      181 tccagcgtac tgctgtaatg cgcctgcaac atgaagaagc gtacggtcat agggctatag
      241 cccctgtcca gcaacggatg tgaaccggag aataattctt cgggaagaaa gctgttaccc
      301 aggctcttgc tcattttctg tccgttcacc gtgagcatat tggtatgcag ccaatagcgc
      361 accggtgtgg tgtggttgca ggccacactc tgcgctacct cattggtatg atgggtagga
      421 ataagatcca tgccaccgcc gtgaatatcg aattgcttac ccagatactt cgtgctcatg
      481 gctgaacact cgatatgcca gccggggtac ccttcacccc agggacttgg ccagcgctgc
      541 aggtgttcgg gttttgcctt gatccacaac gcaaaatcca ggcggccttt cttgagttcc
      601 tgtccgccca ggtctctggt attattcagc atctcttcca gattccggcc actcagctgg
      661 gtatagggat gatctttagc aaatttctct acgtcaaaga acacattgcc atccacctca
      721 tacgcatagc cactttccag aatggctttg gtcatctcga tctgctcgat gatatgtccg
      781 gtagcagtag gttcaatatc cggaggtaag gcgttgaact tcgccatgat gttccggaag
      841 gctgtgctgt atttctgcac gatctccata ggctccacct gttccagccg ggcctgttca
      901 gagatgcggt cgccggtagt acctgctgtg ctttctatat gtccggcatc cgtgatattc
      961 cgcacatacc tgaccttata acccaggtga cgcaggtagc ggtagatcat atcaaaagac
     1021 acgaaggtgc ggcagttgcc caggtgcggt tcgctgtaca cggtagggcc gcacacatac
     1081 atacctacaa acggggcatg cagtggccgg aagacctctt tgcttctggt cgtggtatta
     1141 tagaccagca gttcattctg gtggaacaat tcattgggca tggcacaaaa ataccaagaa
     1201 ggctttaagg ctgcagactg aataccgatc tcacgaggta atgatctgat ccggctgacc
     1261 ttcttcgttc ataatccagt gctttgagca ctgccggcgt atagagcata tagtcgatcc
     1321 gtatgagcgg agcaggcgaa tacgtagctc ccagtcctcg gccacaatgc agaaaagcat
     1381 catccagacc ttcgctgatc ttacggtatg cgtaagaaac gggcgtatca ttgaaatcgc
     1441 cgcagaacag caccgggtgc ggactggcat ccagggcctg tctgatcacc tccacctgtt
     1501 ctgcacgcaa tttatagctg tcacgaagct tatttgccac cctggccggt tcaaaactga
     1561 gcgtatcgct gcttcctgtc tggtctatga actcaaaatc gctgtgggtc agctggttcg
     1621 attgcaggtg ggtggagaag aggcggatgg tatcgccata gatcagtatg tcagcc
//
LOCUS       DAOMAX010000441         1664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_19559_length_1664_cov_3.645121, whole genome shotgun sequence.
ACCESSION   DAOMAX010000441 DAOMAX010000000
VERSION     DAOMAX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19559_length_1664_cov_3.645121"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PK742_13715"
     CDS             <1..1102
                     /locus_tag="PK742_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQU77697.1"
                     /translation="MAAYEPVATGSQMAIQKSTYSADIQNFSSDLLDDQLNTSAVADI
                     ASQSSRPHTAVRHETAESPSSVSSGSAHLAAANGSTSEQTANQESATEALSDDDLLTA
                     DEPVAAISDSELAESLTPEEQEALEQAAAEKEAELAALIAEDEASDAEIEPVEKPKKP
                     AHSPFEGFYIGASGGWHASTMLTGQEVVINDNPIQTGVRFGPSKGLTLGYVFGPNLSL
                     QAEYLYNVVEGQNYYMEVEGAVRSKTMSLYFDQIPVTVKLRTPRTIHALKMPASLNIV
                     AGTQLNILKDYQIPRENKLELEGDIYRKTSFSLLAGAEYEVLVTDHIMVSMGLEGSLS
                     ENVSTSNDPVGSFPKHSISGGAKGGVYYLFGR"
     gene            1099..>1664
                     /locus_tag="PK742_13720"
     CDS             1099..>1664
                     /locus_tag="PK742_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77698.1"
                     /translation="MRTLIAALFSLSAFSCKSRDKACTDHATGLIPVYDLADSSFEGF
                     SGGLYPWGNEIPDFWLQKGLEASGDLIPRGVSGSADTTGKIGFLTLGYSTAAMTARVF
                     YDLCADSSVFTDLSFVTGAQAGLDLTAMTDRTGPYWDSVQARIQEAGMIPEQIQLVWL
                     SSACQELYDRPFPVQATDQIPIYRQVLA"
BASE COUNT          431 a          409 c          429 g          395 t
ORIGIN      
        1 catggccgcc tatgaaccag ttgctaccgg ttctcagatg gccatacaga aaagcacata
       61 tagtgctgat attcaaaatt ttagcagcga cctattagat gatcagttga atacctctgc
      121 tgttgctgat atcgcttctc aaagcagtcg gccgcacact gccgtaagac atgaaactgc
      181 tgagtcacca tcgtcagttt catcaggttc tgcgcatctg gctgctgcaa acggcagcac
      241 ctcagaacag accgcaaacc aggaaagtgc aactgaggcc ttgtcagatg atgatctgct
      301 cactgccgat gaaccggttg ccgcaatttc tgattctgaa cttgctgaat cacttactcc
      361 tgaagaacag gaagctctgg aacaggctgc cgccgaaaaa gaagctgaac tggctgctct
      421 gatagctgaa gacgaagcgt ctgatgcaga aattgaaccg gtagaaaaac ctaaaaaacc
      481 ggcacacagc ccctttgaag gattttatat aggtgccagt ggcggatggc atgcatctac
      541 tatgctcacg ggtcaggaag tggtgatcaa tgataatccg atccagacag gtgtgcgttt
      601 cggacccagc aaggggctga ccctgggtta cgtttttggt ccgaacctgt cacttcaggc
      661 tgagtacctg tacaatgtgg tagaaggtca gaactactat atggaagtgg aaggcgctgt
      721 aagaagcaag accatgtcgc tgtatttcga ccagattccg gtaacggtca agctgcgcac
      781 acccagaacc atacatgcat taaagatgcc ggcttcgctg aacatcgtag ccggaacaca
      841 actgaatatt ctcaaggatt atcagattcc gcgtgagaac aagctggaac tggaaggtga
      901 tatctaccgt aaaacatcct tctctttgct tgcaggtgct gagtatgaag tgcttgttac
      961 agatcacatc atggtttcca tgggtctgga aggcagcctc tcagagaatg tgagcaccag
     1021 taacgatcct gttggcagct tccctaaaca cagcatttca ggcggtgcca aaggcggtgt
     1081 gtactacctc ttcgggcgtt gagaactctg attgctgccc tcttttccct atcagccttt
     1141 tcctgcaaaa gccgggacaa agcatgtact gatcacgcca caggtctgat tccggtgtat
     1201 gatctggctg acagttcctt tgaagggttt tcaggcggac tttacccctg gggcaatgaa
     1261 ataccagact tctggctgca aaaaggactg gaagcttcag gagacctgat accaagaggt
     1321 gtgagcggat cggccgacac aaccggaaag atcgggtttc tgaccctggg ttacagcaca
     1381 gcagccatga ccgccagggt gttttatgat ctgtgcgctg acagttctgt attcactgac
     1441 ctctcttttg taaccggagc gcaggccgga ctggatctaa ctgcgatgac agatcgtaca
     1501 ggaccgtact gggattctgt acaggcaagg atacaggaag ccggcatgat cccggagcag
     1561 attcagttgg tatggctgag ttctgcctgc caggaactct atgatcgccc cttccctgta
     1621 caggctacag atcagattcc gatataccgg caggtgctgg ctaa
//
LOCUS       DAOMAX010000442         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_19952_length_1648_cov_3.259259, whole genome shotgun sequence.
ACCESSION   DAOMAX010000442 DAOMAX010000000
VERSION     DAOMAX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19952_length_1648_cov_3.259259"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK742_13725"
     CDS             complement(<1..805)
                     /locus_tag="PK742_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77699.1"
                     /translation="MKKLITCMIGVSVAFASMAQAPVRTEAREVRPHDRVIVDLEQPS
                     FYKHNPANMAPEALEARSGRGAVEFIPIGTAYNLFTTLLAGQNQVSYHPDINSVAFVH
                     RQNSGTPGGSGGLAFDISTDGGATWTTNVILSLDYNAGAAGVITGNRYPSGSLWNPAG
                     NTDPANAYFVAHGPSLTSVTGSWGATHQMSADFAGGSNYEEYYSEGGLSNDYFPYAIN
                     WYEDGSLWDMKEQLTGDTVYVNKFEFDGVSAFNRTSTMVVPDWEIVNSGG"
     gene            complement(1023..>1648)
                     /locus_tag="PK742_13730"
     CDS             complement(1023..>1648)
                     /locus_tag="PK742_13730"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQU77700.1"
                     /translation="EVYFPVVSPVTMDDGYGNYEIPVVFFQPPADVFYLKGVTLTDED
                     FSPDAVADFGYYQLSSSVSFINNSAYALSYSWDFGDGSPVSTLEDPVHDYPSTGNYTV
                     CLTASNQTSSDTSCELINIIGVENLLNDIWSISPVPAHDNLLIGCNCNLQIDHVQLTD
                     LSGKTTTVPCQPAGDGFIADLTEIPSGQYLVSIWFEGQLWVKTLIVL"
BASE COUNT          464 a          372 c          398 g          414 t
ORIGIN      
        1 caccaccaga gttgacgatc tcccagtcag gaacaaccat ggtagaagtc ctgttaaagg
       61 cacttactcc gtcaaattca aatttgttca cataaacagt gtcacctgta agttgttctt
      121 tcatatccca aaggctgcca tcttcatacc agttgatagc ataagggaag taatcgttag
      181 aaagaccacc ttcagagtag tattcttcat aattgctgcc acctgcaaaa tcagcagaca
      241 tctggtgggt agcaccccat gaacctgtta ctgaggtcag agacgggccg tgagccacga
      301 aataggcgtt agccgggtca gtgttacctg cagggttcca gagtgaacca ctggggtagc
      361 ggttaccggt gatcacacca gcagcacctg cattatagtc aaggctgagg atcacgttgg
      421 tagtccaggt agctccgcca tcagttgaga tgtcaaaggc aagaccgcct gagccaccgg
      481 gagttccgct gttctgacgg tgtacgaaag ccacgctgtt gatgtcagga tggtaagaca
      541 cctgattctg tccggccagc agggtggtga acaggttgta ggctgtacca ataggaatga
      601 attcaacagc accgcgacca gaacgggcct cgagtgcttc cggagccatg ttggccggat
      661 tgtgtttgta gaaagaaggc tgctccaggt ccacaataac gcggtcatga ggccttactt
      721 ccctggcttc ggtacgaaca ggtgcctgtg ccatagaagc aaaagcaacg ctcacaccga
      781 tcatgcaagt aatcaatttt ttcataataa tcggtttaga tttagtttat ctgaatgagc
      841 caaagttaat aaatatgaca aaaaataggg gtatccgcct gtatttatct tgtaaaattg
      901 aagtaatttt taacctgtgc gggtatcagg ccctgatggt ggaatgaacc tgaataaacg
      961 aacaggccgc gaaatggcta ccaggggcat aacccggttg ggtttccggt gcagggtagt
     1021 tatcaaagaa ctatgagggt cttcacccac aattgacctt caaaccatat ggagaccaga
     1081 tattgtccgg atggaatttc tgtaagatct gctataaagc catctccggc aggttgacag
     1141 ggtacagttg ttgttttacc ggatagatca gttagttgta catgatcaat ttgcaaattg
     1201 caattacagc ctataagcaa attgtcatgg gccggaacag ggctgatact ccagatatca
     1261 ttcaacagat tttctacccc tatgatattg atcaattcgc aggatgtatc gctgcttgtc
     1321 tgattactgg cagttaaaca gaccgtgtag ttcccggtag aaggatagtc atgcacggga
     1381 tcttccagcg tgcttaccgg cgacccgtca ccaaaatccc agctgtaaga caaggcatat
     1441 gctgaattat tgatgaaaga aacggatgag ctcagttggt aatatccaaa gtctgctact
     1501 gcgtccggac taaaatcctc atcggtcaaa gtgactcctt tcaggtagaa tacatcggca
     1561 ggaggttgaa aaaataccac cggtatttca tagtttccgt aaccgtcatc catggttact
     1621 ggtgaaacta ccggaaaata tacctcac
//
LOCUS       DAOMAX010000443         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20022_length_1645_cov_3.688679, whole genome shotgun sequence.
ACCESSION   DAOMAX010000443 DAOMAX010000000
VERSION     DAOMAX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20022_length_1645_cov_3.688679"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PK742_13735"
     CDS             <1..538
                     /locus_tag="PK742_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQU77701.1"
                     /translation="FFASVTPRTAGFNTIDMGALLPATILIYFLLMLIGGSPGSTAGG
                     IKTTTFAVTVMNVISIARGKNRVEVFRRELSIGSLRRAFAVISLSLLAIGLSVFLLHV
                     VQPGLDTTQVIFECISAFSTVGLSLGITADLNTASKWVIILTMFIGRVGTLTLFVALF
                     RKVKTLNYRYPTESIMIS"
     gene            549..1238
                     /locus_tag="PK742_13740"
     CDS             549..1238
                     /locus_tag="PK742_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQU77702.1"
                     /translation="MKFIIIGLGNFGAWIGENLTTSGHEVIGVDLSMTKVDAMKDKIT
                     HVMKMDTTDIQALKTLPLKDTDAVIVAIGEDVGASIMTTALLKQLKVKRIIGRVISPL
                     HETVISAIGIDEVVHPEQETAERWSKKLDMKGVVESFNITGQYNIIETKTPLKYVDKT
                     IGECEIREKYKLNIITILRTESKESIFGFEKTVKNAVGVVNSDTIIQGDDILVLFGHI
                     HDLKSFIEDNA"
     gene            complement(1235..>1645)
                     /locus_tag="PK742_13745"
     CDS             complement(1235..>1645)
                     /locus_tag="PK742_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQU77703.1"
                     /translation="GGDDYITKPYSIEELMYKIEVYCKRSGVQTLTIANGEAVNRIGQ
                     YLFVPENFSLLHNGNEVRMTSKETALMSFLIRNKNRLLKRSEILVAVWGSSDDRSSRS
                     LDVFVSRLRKYLKEDENVRLESHKGVGYKLSELA"
BASE COUNT          435 a          406 c          390 g          414 t
ORIGIN      
        1 attctttgcc agtgttaccc cgagaactgc cggcttcaat accattgaca tgggtgcatt
       61 gcttcctgcc accatcctca tctattttct gctgatgctg ataggcggat cgccgggctc
      121 tacagccggt ggtatcaaga ccacgacctt tgccgttacg gtcatgaatg tgatcagcat
      181 tgccagggga aagaaccgtg tggaggtctt cagaagagaa ttgtctatcg gttcactgcg
      241 acgggctttt gcggtgatct ccttatcact gctggccatc ggcttatcgg tttttctgct
      301 gcatgtagta caacccggac tggacaccac ccaggtcatc tttgagtgca tctctgcttt
      361 cagtactgta ggactgtcgc tgggcatcac tgccgatctg aatacagcct ccaaatgggt
      421 catcattctg accatgttca ttggccgggt aggcaccctg accttgtttg tagcgttgtt
      481 cagaaaggtg aaaacactta actatcgtta tcctacagaa tccatcatga tcagctaaat
      541 cacgcaatat gaaattcatc atcataggac ttggaaattt tggcgcctgg atcggcgaga
      601 acctgaccac ctcggggcat gaagtgatcg gtgtggatct cagtatgacc aaagtggatg
      661 ccatgaaaga caagatcacg cacgtgatga aaatggacac caccgatatt caggcattaa
      721 aaacgttgcc cctgaaagac accgatgcgg tgatcgtagc catcggcgag gatgtgggtg
      781 cctccatcat gaccaccgcc ctgctcaaac aactcaaggt gaagaggatc atcggccggg
      841 tcatcagtcc gctgcatgag accgtcatct cagccatcgg tatagatgag gtggttcatc
      901 cggaacagga gaccgcagaa cgatggagca agaaactgga catgaaaggt gtggtggaat
      961 ctttcaacat caccggccag tataacatca tcgaaacgaa aacgccattg aagtatgtgg
     1021 acaagaccat cggcgaatgt gagatcagag agaagtacaa gctgaacatc attaccatac
     1081 ttcgcacaga aagcaaagaa agtattttcg gttttgagaa aacagtcaaa aatgcagtgg
     1141 gtgttgtgaa ctcagatacc atcattcagg gtgatgatat cctggtattg ttcgggcaca
     1201 ttcacgatct gaaatctttt attgaagaca acgcctaagc cagctcagac aacttgtagc
     1261 ccacgccttt atggctttcc agtctgacat tctcatcttc tttcaggtat ttacgcaacc
     1321 tggaaacgaa tacatccaga ctgcgggagc tcctgtcatc actggagccc cacacagcca
     1381 ccaggatctc acttcttttc aatagtctgt tcttgttgcg gatcagaaaa gacatcagtg
     1441 cggtctcttt ggaggtcata cgcacttcat taccattgtg cagcaaggaa aaattctcag
     1501 gcacgaagag atactgccca atgcggttaa ccgcttcacc gttggcaata gtaagcgttt
     1561 gtactccact gcgcttgcag tacacctcga tcttgtacat gagttcctca atgctgtaag
     1621 gcttggtgat ataatcatcg ccacc
//
LOCUS       DAOMAX010000444         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20092_length_1641_cov_4.421816, whole genome shotgun sequence.
ACCESSION   DAOMAX010000444 DAOMAX010000000
VERSION     DAOMAX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20092_length_1641_cov_4.421816"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..480)
                     /locus_tag="PK742_13750"
     CDS             complement(31..480)
                     /locus_tag="PK742_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77704.1"
                     /translation="MKYLILFLILLLTACNRWVVPEELVGTWEAPVSDVAVRTHSKEE
                     GFKFYHDTASAMLIISDNKTVSGRIGSATFENAALKKNFGPPNMTGIAWIVDVGPVGT
                     IHPADPLPQKEVQFWLPPAGETMDAELRYTQNGAQFPMAGMVFRKSE"
     gene            complement(592..>1641)
                     /gene="dprA"
                     /locus_tag="PK742_13755"
     CDS             complement(592..>1641)
                     /gene="dprA"
                     /locus_tag="PK742_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQU77705.1"
                     /translation="IGFIIARNLIEYLGSARAVWETPPRELLRIPGIYKHVLKAIRDA
                     DVFATADTELAWMHKNNVQFLHFWHPEYPERLRHCPDAPLFLCYRGNGSLNHHRQVAI
                     VGTRHATPYGRRITEELVAGLLELDCLIVSGLAYGIDIAAHKAALDNGLQTIGVVAHG
                     LDRVYPAMHKEVARQMEENGGLLTEILPGNDPDRQNFPRRNRIVAGMVDALVVVESGE
                     NGGAIITASLANGYNRDVFAFPGQVHHAFSQGCNLLIQRQQAQLITCADDLVRAMNWK
                     SDPEKKKGQRQLFLELNAEEKLVYDILRNKGELHIDDLTLRTGLSPGTLAGTLLSLEF
                     QGVIQTLPGKRYKLD"
BASE COUNT          376 a          448 c          378 g          439 t
ORIGIN      
        1 aaacattaac gtcccgtagc acgaaaggta tcattcgctt ttcctaaata ccattcctgc
       61 cataggaaac tgagcaccgt tctgtgtata cctgagttct gcatccatgg tttcccctgc
      121 aggtggaagc cagaactgaa cttccttttg tggcaatggg tctgccggat gaatggttcc
      181 aaccggtccg acatccacga tccaggcaat gccggtcata ttaggaggac cgaagttctt
      241 tttgagggct gcattttcaa atgttgcact gcctattctt cctgaaaccg tcttattatc
      301 agaaatgatc aacatggcag aggcggtatc gtgataaaac ttaaaaccct cttctttcga
      361 atgtgtcctg acggcaacat ctgatacagg tgcttcccat gttccaacca actcttcagg
      421 caccacccag cgattacagg cggtcagcag caggataaga aacagtatca agtacttcat
      481 ataagacccg ttttatgatg caacatacgg ttgatatgaa tcatataaaa tgacttttat
      541 gtggtttcac ccctctgaga ggggggacaa cccccattcc gtactcccga ttcaatccag
      601 cttgtaccgc tttcccggca gtgtttgtat cactccctga aattcgaggc tgagcagcgt
      661 gccggccagg gtaccagggc tcaaacctgt tcttagggta aggtcatcaa tgtgcagttc
      721 tcctttattt ctgagtatat catataccag cttctcttct gcattcagct ccaggaataa
      781 ctgtctttgt cctttctttt tctccggatc acttttccag ttcatggctc tgacaaggtc
      841 atcggcacag gtgatgagtt gtgcctgttg tctttgtatc agcaggttgc atccctggct
      901 gaaagcatga tgcacctgtc cgggaaaggc aaatacatcc ctgttgtaac cgttggccag
      961 cgaagccgtg atgatggcac cgccgttctc tccagattcc accactacca gggcatccac
     1021 catccccgcc acgatcctgt ttcgtcttgg aaaattttgt ctgtccggat catttccggg
     1081 caggatctcc gtcagcaacc caccattttc ttccatctgc ctggccacct ccttatgcat
     1141 tgccggatac acccggtcca gtccgtgagc taccacacct atagtttgca gcccgttgtc
     1201 cagtgcggct ttatgtgctg ctatgtcaat gccataagcc agtccgctca cgatcaggca
     1261 atccagctcc agcagaccgg ctaccagctc ctctgtgatc cttcggccgt acggtgtggc
     1321 gtgtcgggtg cccactatgg ccacctgtct gtgatggttc aaagaaccgt ttccgcgata
     1381 gcagagaaat aagggtgcat ccgggcaatg cctcagtctc tccggatatt ccggatgcca
     1441 gaagtgcaga aactgaacgt tgtttttgtg catccatgca agctcagtat ctgcagtagc
     1501 aaagacatca gcatcacgaa tagctttcag aacatgcttg tagatgccgg gaattcggag
     1561 cagctcacgg ggtggggtct cccataccgc acgggcactg cccaggtatt cgatcaggtt
     1621 gcgggctatg atgaaaccga t
//
LOCUS       DAOMAX010000445         1634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20250_length_1634_cov_4.613046, whole genome shotgun sequence.
ACCESSION   DAOMAX010000445 DAOMAX010000000
VERSION     DAOMAX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20250_length_1634_cov_4.613046"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..304)
                     /locus_tag="PK742_13760"
     CDS             complement(101..304)
                     /locus_tag="PK742_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQU77706.1"
                     /translation="MKKNMGFADRIIRIIIAAVLAILYFTDVVSGTLGTVALVVAVIF
                     VLTSLVSFCPIYSIFGMKTCKTE"
     gene            complement(460..1224)
                     /locus_tag="PK742_13765"
     CDS             complement(460..1224)
                     /locus_tag="PK742_13765"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQU77707.1"
                     /translation="MISQKKCREYRVELEEVLREIAAVSEQPKFESLQDYLGTGIPVL
                     GLRSAQINDCFRTGFPWDELPMDDQWAVWQHIWFESQVFEAMSLALIFVRKRARKIGQ
                     ERLHSDLPAWVDRVDNWAHSDELSHAYAVMLEQEQKRIFSMLKKWNRDSDPWKRRQSL
                     VVLIRRRNKHTLAFEEMIPLIERLVSDDDYFVQKAVGWALRDIALVFPEPTETWVEAH
                     VAELSSPAFATASERTHPTLKEAWKEVRRMERKRRK"
     gene            complement(1221..>1634)
                     /locus_tag="PK742_13770"
     CDS             complement(1221..>1634)
                     /locus_tag="PK742_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQU77708.1"
                     /translation="FGLTLHAFLEGVPLSGSDIGHDHAEHVYYAILIHKLPEAFSLAT
                     VLFFSLKKHSHAAIILLLFTLVTPAGAWAGHFLSERSAGHSINWLLAIVCGTFIHIST
                     TIIFESSGKAHHISLYKFLAILLGTGLSLIGLLGT"
BASE COUNT          422 a          431 c          369 g          412 t
ORIGIN      
        1 tcctaacgca tcccgttagt ggaacaaata catgactccc cgaatatgac ataaaaaaaa
       61 cgccatgtga acatggcgtt ttcattcttt ttgatccgga tcattctgtt ttacaggtct
      121 tcattccaaa tatggaatag atcgggcaga aactgaccag gcttgtcagc acgaatatga
      181 ctgctacgac caacgccaca gtacccaggg ttcctgaaac tacatcggta aagtacagaa
      241 tagccaaaac tgccgcaatg atgatgcgga tgatccggtc ggcaaagccc atgttctttt
      301 tcatagtttg tggttttgga tggttattga agcagccaga cgatcagcgt aatgagcccc
      361 agacagagca cacatgacat gatcaacttt actgccttgt tcatgattac ttgcaaattc
      421 aaaaataagc tcctgaaaca cgtataatgg tgacgatagt cacttgcggc gcttcctttc
      481 catccgcctt acttccttcc aggcttcttt caatgtgggg tgggtgcgtt ctgaagcagt
      541 tgcgaaagca ggactgctta gttctgccac atgggcttca acccaggtct cggttggttc
      601 aggaaacacc agtgcaatat cccgaagtgc ccagccaaca gctttctgga cgaaataatc
      661 atcgtcagat accaggcgct caataagcgg gatcatttct tcaaatgcga gggtgtgttt
      721 attccggcga cggatcagca ccaccaggct ttgcctgcgc ttccatggat cgctgtcgcg
      781 gttccatttc ttcaacatcg aaaaaatacg cttttgttcc tgctccaaca tcaccgcgta
      841 cgcatgcgat aattcatcag aatgtgccca gttgtccacc cggtccaccc aggctggcag
      901 gtcactgtgc aatctctcct gcccgatctt ccgggctcgc tttcgcacaa atatcagtgc
      961 cagcgacatg gcctcgaaca cctggctctc aaaccagata tgctgccaca cagcccattg
     1021 atcatccata ggcagctcgt cccacggaaa tccggttcgg aagcagtcgt tgatctgagc
     1081 cgatcgtaaa cccagaactg gtatgccggt tccaagataa tcctgcaggc tttcaaactt
     1141 cggttgctca cttacagctg ctatttccct aagcacctct tccagttcaa cccggtattc
     1201 tctgcatttt ttctgactga tcatgtacca agtaatccga ttaatgatag ccctgtgccc
     1261 aacagaatgg ccaggaactt atatagactg atatgatgtg ccttaccact cgattcaaag
     1321 atgatggtgg tggagatatg aatgaaagta ccacatacga ttgccagtaa ccagttgatg
     1381 ctgtgtcccg ccgaccgttc actcaggaaa tgacctgccc aggcacctgc cggcgtaacc
     1441 agggtgaaca gcaacagtat gatggctgca tggctgtgct tcttcagcga gaagaacaac
     1501 acagttgcca gtgagaaggc ttcaggcagc ttgtggatga gaatggcata atacacatgt
     1561 tctgcgtggt catgtccgat atcacttcct gacagcggaa ctccttccag aaaggcatgc
     1621 agggtcagtc cgaa
//
LOCUS       DAOMAX010000446         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20408_length_1628_cov_3.678322, whole genome shotgun sequence.
ACCESSION   DAOMAX010000446 DAOMAX010000000
VERSION     DAOMAX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20408_length_1628_cov_3.678322"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1593
                     /locus_tag="PK742_13775"
     CDS             217..1593
                     /locus_tag="PK742_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQU77709.1"
                     /translation="MQRRDFVRNSAILSMAGLISPSSLFAQYSVKEKVRLGFIAVGFR
                     GQNHIEEMLKRNDVEIVAFADPDPGMMIHAQAMLEAAGKPAAAEYGNGDYDYRNLLAR
                     DDIDAVFVSSPWEWHLPHGVDAMRAGKIVGMEVCGAMTVEDCWTYVRVHEETGVPIMM
                     LENVCYRRDVMAVLNMVRQDMFGELIHGQGGYEHDLRGVLFNDGETAYNSGVEFGEKG
                     YSEAKWRTSHYVKRNGELYPTHGLGPLATMFDINRGNRMVRLTSMASKSRGLHKYIVE
                     HEKGGPDHPNAQVEFKQGDVVVTQIQCANGETLMLTHDTSLQRPYNLGFRVQGTEGLW
                     QDFGWGRADQGFLYMEKRMNHSHQWENTEEWFQKYDHPLWARYSAEAGGSGHGGMDFF
                     VDNAFIECIKSNKEFPLDVYDLAAWYVITPISEASVANDNAPQDIPDFTRGKWQERKP
                     VFGLTDAW"
BASE COUNT          431 a          364 c          459 g          374 t
ORIGIN      
        1 acacaggatc gccgcaggca atcctgacgg actaatgcca agcctaagct tgagcaactg
       61 actctaaaga aaaacagcga cagcgcagct gccgccggtt ttgcattcgg aattcggacg
      121 ggcacaggat ggccgcaggc aatcctgaca ttgaatgcca agcttcagct ttaggacttt
      181 cacctcaatt tcctattttg acctccaaat tgtacgatgc aacgaagaga ctttgtaaga
      241 aactcagcga tcctgagtat ggccggactg atcagtccgt cttcactctt tgcccaatac
      301 agtgtaaaag aaaaggtacg tcttggtttt atagctgtgg gtttccgtgg tcagaaccac
      361 atagaggaaa tgctgaaaag aaatgatgtg gagatcgtag cctttgctga tcccgatcct
      421 ggtatgatga tacatgctca ggcaatgctg gaagctgcag gaaaacccgc tgcggctgag
      481 tatggcaacg gcgactacga ctatcggaac ctgctggccc gcgacgacat cgatgcggtg
      541 tttgtgtcgt ctccatggga atggcacctt ccacatggag tagatgccat gcgtgcagga
      601 aagatcgtcg gaatggaagt ttgcggggcc atgacagtgg aagactgctg gacctatgtg
      661 cgtgtacatg aagaaacagg cgttcccatt atgatgttgg agaacgtttg ctatcgcagg
      721 gatgtcatgg ctgtactgaa tatggtcaga caagatatgt tcggcgaatt gatccacggt
      781 cagggtggat atgaacatga cctgcggggt gtgctgttca acgacgggga aacggcctat
      841 aactcaggtg ttgaattcgg cgagaaaggc tacagcgaag caaagtggcg aaccagtcat
      901 tatgtaaaac gcaatggcga actctacccg acacatggcc tgggtcccct ggccaccatg
      961 ttcgatatca accgcggcaa ccgcatggtg cgactgacca gtatggcgag caagtcgcgg
     1021 ggcctgcaca aatacattgt ggaacatgaa aagggtgggc ccgatcatcc caatgctcag
     1081 gtggagttca aacaggggga tgtggttgtc acgcagatcc agtgtgccaa cggcgaaacg
     1141 ctgatgctga cacacgatac ttcgcttcag cggccctata acctgggttt ccgtgtgcag
     1201 ggaacagagg gtctctggca ggatttcggc tggggaagag ctgatcaggg tttcctgtat
     1261 atggaaaaac ggatgaatca ctcgcaccaa tgggaaaata cggaagagtg gttccagaag
     1321 tatgatcatc cactctgggc aaggtactct gcagaagccg gtggatcagg gcatggcggt
     1381 atggacttct ttgtagataa tgcctttatc gagtgcatca agagcaataa ggaatttcct
     1441 ttggacgtat atgatctcgc cgcctggtat gttattacac caatcagtga agcttctgtt
     1501 gcaaatgaca atgcccctca ggatatacct gatttcacca ggggaaaatg gcaggaaaga
     1561 aaacctgtgt tcggattgac cgatgcctgg taaagacatc acattattag ttctgggagg
     1621 tgggttat
//
LOCUS       DAOMAX010000447         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20434_length_1627_cov_3.545165, whole genome shotgun sequence.
ACCESSION   DAOMAX010000447 DAOMAX010000000
VERSION     DAOMAX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20434_length_1627_cov_3.545165"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PK742_13780"
     CDS             <1..572
                     /locus_tag="PK742_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77710.1"
                     /translation="HIEIHGQKGYHGVAIASRRPLSNSRRRDFCAKGDARHICVALKV
                     GARNIELHNFYVPHMKVKMDAVPGMPTSFKFTPTITTEEMKQILSQNPAWQRVREGDT
                     DPVWMSFKYEVACAELCGLGHSAMKYTMVVESEDEYNTWAESQIPYWNGIASSLKISG
                     FSKAAPVLKTAEPEAPAATDTTATVIAAQ"
     gene            593..>1627
                     /locus_tag="PK742_13785"
     CDS             593..>1627
                     /locus_tag="PK742_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQU77711.1"
                     /translation="MSQETTIKEHLETHVTDHDHDHDHGHHDHHQSFISKYIFSTDHK
                     TIAKQFLVTGIIWAIVGGGLSVLFRIQLGYPDADLSWMKPLLGKWITPEGHIAPEFYN
                     ALITMHGTILVFFVLTAGLSGTFSNYLIPLMIGARDMASGFINMLSYWFFFLSSVVMA
                     VSFFLATGPASGGWTAYPPLNALPAAASGSVAGVDYWLVSIVLFIVSVLLGGLNYITT
                     VINLRTRGMKMFRLPLPIWAFFLTAVIGLLSFPVLLSAGVLLLFDRNLGTSFYLDQIY
                     IGGEALANAGGSPILYQHLFWFLGHPEVYIIILPAMGIASEVLSVHSRKPIFGYTAMI
                     YSLLAIAVLSF"
BASE COUNT          377 a          391 c          406 g          453 t
ORIGIN      
        1 agcacatcga gatccatggc cagaagggct accatggcgt cgcaatcgct tcgcggcgac
       61 cgctatcgaa cagcaggcgg cgcgatttct gcgccaaggg cgatgcgcgc catatttgcg
      121 tcgctctgaa ggtcggagcg cgcaatatag agctgcacaa tttctatgtg ccgcatatga
      181 aggttaagat ggatgctgtt cccggtatgc ctacttcatt caagttcaca cctaccatca
      241 ctaccgaaga aatgaaacag atcctgagtc aaaatccggc ctggcaacgg gttagagaag
      301 gagatacgga tcctgtatgg atgagcttta agtatgaagt ggcttgtgct gaactttgcg
      361 gattaggtca ctcagccatg aagtacacca tggtagtgga aagtgaagat gaatataata
      421 catgggctga atcacaaatt ccatattgga atggaattgc ttccagtctg aagatttccg
      481 gtttcagtaa agcagcgccg gtgttgaaaa cagctgaacc ggaagcacct gctgccactg
      541 atactaccgc aactgtaatt gctgctcaat aatattttaa acactgttcg atatgtccca
      601 agaaacgacg atcaaggagc acctggaaac gcacgtgacc gatcatgacc atgaccacga
      661 ccatggtcat catgatcatc atcagagttt tatttccaag tacatttttt caacagatca
      721 caaaacaatt gccaagcagt tcctggtaac aggtatcatt tgggcaattg tgggtggagg
      781 actttctgtg ttgttcagga tacagcttgg ttatccggat gctgacctga gttggatgaa
      841 gccgctgctg ggaaagtgga tcactccgga aggacatatt gctcctgaat tctataatgc
      901 gctcattacc atgcatggta ccatcctggt attctttgta ctgaccgcag gtctgagtgg
      961 tacattctcc aattatctca ttcctttgat gatcggtgcc agggacatgg catccgggtt
     1021 tatcaatatg ttgtcttact ggttcttctt cctgtccagt gtggtcatgg ccgtttcttt
     1081 cttcctggcc acaggacctg cttccggagg atggaccgcc tatccgccac tgaatgccct
     1141 gccagcagca gcctcgggtt ctgttgccgg ggtcgattac tggctggtga gtattgttct
     1201 gttcattgta tccgtgctgc tgggtggttt gaactatatc actacagtga tcaacctgcg
     1261 tacccgtggt atgaagatgt tcagattgcc actgcccatc tgggcctttt tcctgactgc
     1321 agtgatcggt ctgttgtcat tcccggtact gctgtctgca ggtgtgctcc tgttgtttga
     1381 cagaaatctg ggtaccagtt tctatctgga tcagatctac atcggcggtg aagcactggc
     1441 gaacgccggt ggcagcccga tcctctacca gcatttgttc tggttcctgg gtcacccgga
     1501 agtgtatatc attatcctgc ctgccatggg tatcgcttca gaggtactat ccgtgcattc
     1561 acgcaaaccc attttcggat atactgcgat gatctattca ctgctggcta ttgcagtact
     1621 gtcattc
//
LOCUS       DAOMAX010000448         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20576_length_1621_cov_3.226692, whole genome shotgun sequence.
ACCESSION   DAOMAX010000448 DAOMAX010000000
VERSION     DAOMAX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20576_length_1621_cov_3.226692"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PK742_13790"
     CDS             <1..783
                     /locus_tag="PK742_13790"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQU77712.1"
                     /translation="AYDDFPHTGSMRYLDPEQQIPAVALSTMDADVLKEALQQNADLQ
                     FRLVTYSQTLEMVPSFNVIGELRGTTYPDEVLVVGGHLDSWDVGEGAHDDGAGCVQSM
                     EVLRTLRASMGQPKRTVRCVLFMNEENGVKGGHAYADWAGTTGQKNLFAMESDAGGFT
                     PRGFSLTDSAYVSYFQGLKPLFEPYNIHYIEYGYGGTDIHPLEDHGTLMAGLVPDSQR
                     YMDYHHTENDIFEHVNKRELELGAGTMACLVWYISEYGLPVH"
     gene            888..1574
                     /locus_tag="PK742_13795"
     CDS             888..1574
                     /locus_tag="PK742_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQU77713.1"
                     /translation="MKKVFFTVLLAGIVAFSAQAQLQVGAKVGINLANQSSDVEGYES
                     QSALGLIFGVPVRLMLSDNLAFQPEVSFMQKGSSSDYSFELPGVYSIESSGKGIINYL
                     EIPLMVQYLFGNGDIMPFVTAGPSIGLGLGYSSKGESTTTSIDFFTGEETTVTEDYDD
                     SGSMEDAGLSAIDFGVGFGAGAVMGMGNGAVIFQVGYLLGLANIYDEGDAKLNNRGIN
                     ITVGYLIGMN"
BASE COUNT          423 a          353 c          411 g          434 t
ORIGIN      
        1 gcttatgacg attttccgca cacaggcagc atgcgttatc tggatccgga acaacagatc
       61 cctgcagtag cactcagcac catggatgca gatgtgctga aagaagcctt gcaacaaaac
      121 gctgacctgc agttccggtt ggtcacctac tctcagacat tggagatggt accatcattt
      181 aacgtgatag gcgaactgcg tggtaccact tatccggatg aagtgctcgt agtgggcgga
      241 catctggaca gctgggatgt aggcgaaggt gcccacgatg acggtgcagg ctgtgtgcaa
      301 agcatggaag tgcttcgaac acttcgggcc agtatggggc agccaaaaag aacggttcgc
      361 tgcgtcctgt tcatgaatga ggagaatggt gtaaagggtg gacatgccta tgccgactgg
      421 gcaggaacca ccggtcagaa aaatctcttc gccatggaaa gtgatgcagg aggatttacc
      481 ccaaggggat tcagcctgac cgactctgcc tatgtctcct attttcaagg tctcaaacct
      541 ttgtttgaac cctacaacat ccactatata gaatatggtt atggtggaac tgatatccac
      601 ccgctggaag atcatggtac gctgatggca ggcctggttc ctgattcaca gcgttacatg
      661 gactaccacc acacagaaaa tgacatattt gagcatgtga acaaaagaga actggaactg
      721 ggggccggta ctatggcatg tttggtctgg tatatcagtg aatatggcct tccggtacat
      781 tagaccatgt tatctgctgg acactttttt acacaattgt gaataattgc tgcgatttgt
      841 acaattttat accattcttt gatcctataa taaccaaaca cactattatg aaaaaagtct
      901 tttttactgt tctcctcgct ggtattgttg ctttttcagc acaagcacag cttcaggtag
      961 gtgctaaagt gggcatcaac ctggccaacc aatccagtga tgtggaaggc tatgaatctc
     1021 aaagtgccct gggcctcatt tttggagtac cggtgcgcct gatgctgagc gataaccttg
     1081 cattccagcc ggaagtaagc tttatgcaga aaggttcttc atctgattat tcttttgaat
     1141 tgcccggagt gtattcaatt gaatcaagcg gaaaaggtat catcaactat ctggagatcc
     1201 ctttgatggt gcagtacctg tttggtaacg gagatatcat gcctttcgta actgcaggtc
     1261 ccagcatagg tctgggtctg ggttatagca gcaaaggaga atccactacc acttcgatcg
     1321 atttcttcac cggtgaagaa actaccgtaa ctgaagatta cgatgattca ggcagcatgg
     1381 aagatgcagg cctgagtgct atcgatttcg gtgttggttt tggtgctggt gctgttatgg
     1441 gcatgggcaa cggtgctgtt attttccagg taggatacct gctcggactg gcgaacatct
     1501 atgatgaagg tgatgccaaa ctgaacaacc gtggtatcaa tattaccgta ggatacctga
     1561 tcggtatgaa ctgatcctga acgtatttaa aaaaagccgg ctgcatgcag ccggcttttt
     1621 t
//
LOCUS       DAOMAX010000449         1618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20641_length_1618_cov_3.153551, whole genome shotgun sequence.
ACCESSION   DAOMAX010000449 DAOMAX010000000
VERSION     DAOMAX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20641_length_1618_cov_3.153551"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PK742_13800"
     CDS             complement(<1..1188)
                     /locus_tag="PK742_13800"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQU77714.1"
                     /translation="MRCRLLIALLLPVSLWAQTASLSGVISDSETGELLPGVNIILTG
                     TNFGAATDFDGKYSIDGIPAGEYNIQISFIGYEQLLFTGQKFVAGEKKKLDVTLNSTS
                     VIFEDAVVIIGEKPLVDVEDASTGSSMNKEQIEMAPTRQIQNLLNTQTGVVQSSDGIH
                     IRGGRNYETGFYIDDVSARDPLAGTGFGIDIGSNAIEKVDVTTGGAGVEYGNATAGVV
                     NTTTRSGEDQFEFNATYKRDNFGFNADTRSNWEQQVMELGLGGPLAFKKKLTYYSTLR
                     LNFSDTYIQNPADQVVSSLYPDTRWSPYQDNRWAGMLKLNYNINPRQRLAFTYLKSIT
                     INQDYNMLRITGNDITFNPGYQFYFDLQPDNANTYTHDTNLESLRWFHTVSNTFSYNL
                     TFSR"
     gene            complement(1300..>1618)
                     /locus_tag="PK742_13805"
     CDS             complement(1300..>1618)
                     /locus_tag="PK742_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77715.1"
                     /translation="KAYQQVSGVCRTVRGPSMSCTPGKVQVLGVNEMPLNGKMEKVIT
                     LRMIQGRNPDWVARPFFAKYDPNAKWMNDLKPAFGDTFFFEQELEQMYHEKVDQGEHA
                     NFE"
BASE COUNT          366 a          392 c          422 g          438 t
ORIGIN      
        1 tcgggagaag gtcaggttgt agctgaaggt attgctgacc gtatggaacc agcgcagact
       61 ctccaggttg gtgtcgtggg tataagtgtt ggcattgtcc ggctgaagat cgaaatagaa
      121 ctggtaaccg ggattgaaag tgatatcatt ccctgtgatg cgcagcatgt tgtagtcctg
      181 gttgatggtg atggacttca ggtaggtgaa ggccagtcgt tgcctcggat tgatattgta
      241 gttcagcttg agcattcctg cccagcgatt gtcctgatag ggactccagc gggtgtcggg
      301 atagagcgaa ctgaccacct ggtctgccgg attctgtata taggtatcac tgaagttcag
      361 tcgcagggta gagtagtagg tcagtttctt tttgaaggca agcggaccac ccaggccgag
      421 ttccatgacc tgttgttccc agttggaacg ggtatctgca ttgaaaccga aattatcccg
      481 cttgtaggtc gcattgaatt caaactggtc ttctccggaa cgggtagtgg tattgaccac
      541 acctgcagtg gcattaccgt actccacacc ggcgccaccg gttgtaacgt ccaccttttc
      601 aatggcattg ctacctatat caataccgaa tcctgtgcct gcaagcgggt ctcgggcaga
      661 cacatcgtcg atatagaatc ctgtttcgta gttgcggcct cctctgatgt ggatgccatc
      721 ggatgactga accacgcctg tctgtgtgtt gagcagattc tggatctggc gggtgggtgc
      781 catctcgatc tgttccttgt tcatagagga tcccgtgctg gcatcctcca catccaccag
      841 gggtttttcg ccgatgatga ccaccgcatc ttcgaaaatg acagaggtgg aattaagtgt
      901 tacatccagt ttcttctttt cgcctgctac gaatttctgt ccggtgaaca gcagctgctc
      961 atagcctata aaggagatct ggatattgta ttcgccggcg gggatgccat ctatggaata
     1021 cttgccgtca aaatcagtgg ccgcaccaaa gttggtacct gtcagaatga tgttgacacc
     1081 gggtagtaat tcaccagttt cgctatcact gatcacacca gacagacttg ctgtttgtgc
     1141 ccacagggat acaggcagca ggagggcgat caggaggcgg catctcatcc ccgcaaattt
     1201 gctgataaaa tgggaatgaa acggcccgga ttgtccacct gcagaaaaga aaaaggaata
     1261 gttcacacta atccttttct gtattaacaa tggcttgatc tattcgaaat tggcatgctc
     1321 accctggtcc actttttcgt ggtacatctg ttcgagctcc tgttcaaaaa agaaggtatc
     1381 gccaaatgca ggtttcaggt cattcatcca tttcgcattc gggtcatatt tggcaaagaa
     1441 aggtctggcc acccagtcgg gattgcgacc ctgtatcatg cgcagggtga tcactttttc
     1501 catttttccg ttcaggggca tctcattcac tccgagcacc tgcacctttc cgggcgtaca
     1561 gctcatgctg ggaccacgca ctgttctgca aacaccgctt acctgttggt aggccttt
//
LOCUS       DAOMAX010000450         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20719_length_1615_cov_3.114744, whole genome shotgun sequence.
ACCESSION   DAOMAX010000450 DAOMAX010000000
VERSION     DAOMAX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20719_length_1615_cov_3.114744"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..903
                     /locus_tag="PK742_13810"
     CDS             118..903
                     /locus_tag="PK742_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQU77716.1"
                     /translation="MRMNQQTTIVLLRTDMGKLVWRPLGMNKFISGLVGLGLYDWTGM
                     LIGLLIGSVLDAKFIPKPAAEKEVNLQLHYMMLGVYVLQRCNGFRVIPISEILRRFNM
                     FLGADFVAPRIRMLEDMSRQRIQVEAACKQIEANALPTTRDWLLQQLAGLSDHPGVQQ
                     NYRKAVLKQVADRLGRGIPIPEEETKKAPPPPRKPAYSRTDSLYRKLECQPGDDHATV
                     KKAYYRLAKIHHPDRNQHSPMSVVRFREIKEAYEGLCRVKGWK"
     gene            complement(905..1393)
                     /locus_tag="PK742_13815"
     CDS             complement(905..1393)
                     /locus_tag="PK742_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77717.1"
                     /translation="MKAVIFSIIILVYSNIHSQIPGKLLSTDPLVEGPVSFRTLQATY
                     DTLYPVADGRFITHNNNYWGVTDSAGLVYLPFQFNGITYVDGKGYLCSTYLESYSLNT
                     GIPRYSYTGLAIQYDENGKKKRQYRFNLTVEFTSDYFWQEQVTLYGPYFFLPEQYRKR
                     IY"
     gene            complement(1390..>1615)
                     /locus_tag="PK742_13820"
     CDS             complement(1390..>1615)
                     /locus_tag="PK742_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77718.1"
                     /translation="RPLTWQQLVFTYLIPVIPIMYAWDGQASLVRTYTFDDIRELTGS
                     PSTDYVWDVGPAMNAKGKRSGYYIFGHPVK"
BASE COUNT          421 a          398 c          402 g          394 t
ORIGIN      
        1 atgcaggagt tgacaactgt tctgtttccc accgggtgat gtggatgtca ttccagccaa
       61 tgtttcagaa cggacggcgt ttatgagcat aaattattct gccattactt ttgcctgatg
      121 cgtatgaacc aacaaaccac catagtactg ttgcgcacag acatgggcaa gctggtttgg
      181 cgccccctgg gtatgaataa attcatatcg ggtctggtgg gtctgggttt atacgactgg
      241 acaggaatgc tcatcggcct gcttatcggt tccgttctcg atgcgaaatt catacccaag
      301 cctgctgctg aaaaagaggt caatctccag ctgcattata tgatgctggg tgtctatgta
      361 ctgcaacgtt gtaatggttt ccgggtcata cctatctcag agatactgcg ccggttcaat
      421 atgtttctgg gtgccgattt tgtcgcacca cgcatccgga tgctggaaga tatgtcgagg
      481 cagcgcatcc aggtggaagc tgcctgcaaa cagatcgaag ccaacgccct gccaaccaca
      541 cgagactggc tgttgcagca actggccggc ctgagcgacc atccgggtgt tcagcagaac
      601 tatcggaaag cagtcctgaa acaagtggca gaccggctgg gacgtggtat ccctataccg
      661 gaggaagaga ccaaaaaggc tccgccgcca cccaggaaac ctgcatacag cagaacagac
      721 agcttataca ggaaactgga gtgccagccc ggcgacgacc atgccacggt caaaaaagcc
      781 tattaccggt tagccaagat ccaccatcct gaccgcaacc aacacagccc gatgagcgtg
      841 gtcagattcc gggagatcaa agaagcatat gaaggattgt gcagggtgaa ggggtggaag
      901 tgagtcaata tatcctcttc cgatactgct ccggtagaaa gaaatatgga ccatacaagg
      961 ttacctgttc ctgccagaaa taatcgctgg tgaactccac ggtcagattg aatctgtatt
     1021 gccttttttt cttcccattt tcatcgtact gaatggcaag acccgtatag ctgtatcgtg
     1081 gtattccggt attcagggaa taactttcca ggtaggtgct gcacaaatag cccttaccat
     1141 ccacgtaggt gatcccgttg aattgaaaag gcagatagac cagtcctgcg ctgtctgtca
     1201 ctccccagta attgttgtta tgcgtaatga accttccatc agcaaccgga tacagggtat
     1261 cataggtggc ttgcagtgtc ctgaatgata cggggccttc taccagcgga tcagtggaca
     1321 acagcttacc tggtatctgc gagtggatat tggaataaac taatattatt atactaaata
     1381 ttactgcctt cattttacag gatgaccaaa aatgtaatac ccggatctct ttcctttggc
     1441 gttcatggcc ggccccacat cccagacata gtcggttgat ggcgaacctg tcagctcccg
     1501 gatatcgtcg aacgtatagg tacgaaccaa tgaggcttgt ccgtcccagg catacatgat
     1561 aggtatgacc ggtatcagat aagtaaatac cagctgctgc catgtcaaag gcctg
//
LOCUS       DAOMAX010000451         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20857_length_1608_cov_4.445589, whole genome shotgun sequence.
ACCESSION   DAOMAX010000451 DAOMAX010000000
VERSION     DAOMAX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20857_length_1608_cov_4.445589"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK742_13825"
     CDS             <1..426
                     /locus_tag="PK742_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77719.1"
                     /translation="FLDPLYLSSDTAAANLHTEGSAAYNKGNPETITDVLETDLDDQP
                     RVLNDTIDCGAYEYYSEPVGIADLQSSILIYPNPAHQFIHLEAHSYISARMYDESGKI
                     IGFWQPVPQQIPTDKLLPGYYLLVLDNGLQWHTFPVVVE"
     gene            457..>1608
                     /locus_tag="PK742_13830"
     CDS             457..>1608
                     /locus_tag="PK742_13830"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQU77720.1"
                     /translation="MRFLFLLLAAAAFPASAQNILISDALNPNEPSIMFNPKDPAIMI
                     AGSNLNSYYLSSDTGKTWTQHLLTSSFGVWGDPVIEVDTAGVFYFFHLSNPPGGNWID
                     RIVCQKTEDNGATWNDGSFTGLNGTKAQDKQWSSVNAHNNHIFLTWTQFDQYGSFNPA
                     DSSIILFSRSFDQGETWSEAKRINQHAGDCIDSDNTVEGATSAVGPDGELYVVWAGPD
                     GLIFDRSYDEGDTWLDEDIIIGEFPGGWDYAIPGLFRCNGLPILKCDTSGGPNNGTLY
                     LNWTDQRNGEEDTDVWLAKSTDGGDTWSDPVRVNDDDAGKQQFMTWMDVDQVNGHLFF
                     VFYDRRNYDDNRTDVYVAYSFDGGETFTNERISDSPFTPNEGVFFGDYTN"
BASE COUNT          418 a          411 c          389 g          390 t
ORIGIN      
        1 tttctggatc cgttgtatct ctcatcagat accgctgccg ccaacctcca tactgaagga
       61 agtgcagcct ataataaggg caacccggaa acgataaccg atgtactgga aacagacctg
      121 gatgatcagc cacgggtgtt gaatgacacc atcgactgtg gcgcctatga atattattca
      181 gaaccggttg gcatcgcaga tctgcaaagc agcatcctca tttatccgaa tcctgcccac
      241 caattcatac atctcgaagc acattcctat atatcggcaa ggatgtatga tgagtctgga
      301 aagatcatcg gcttctggca accggtacct caacaaatac caacagacaa actccttcca
      361 ggatactatt tgctggtgct ggacaatggc cttcaatggc atacgtttcc agttgtagtg
      421 gaataggctg ctcaggtaac cttcgtatct ttaaggatgc gtttcttatt tctcctcctg
      481 gcagcagcag cctttccggc ttctgctcag aacattctga tcagtgatgc gctcaatccc
      541 aatgagccaa gcatcatgtt caatccgaaa gatcctgcca tcatgatagc aggctccaac
      601 ctgaacagct attatctaag cagtgatacc gggaaaacct ggacacaaca tctgcttaca
      661 tcttcttttg gtgtctgggg tgaccctgta attgaggtag atactgcggg tgttttctat
      721 ttcttccatc tgagtaatcc acccggtggt aactggatag accgcatcgt ctgtcagaag
      781 accgaagaca acggagctac ctggaatgac ggcagtttta caggacttaa tggcaccaag
      841 gcacaggaca aacaatggag ttctgtcaat gcacacaaca accatatctt tctcacctgg
      901 acccagttcg atcagtacgg atcttttaat ccggcagatt cctctatcat actcttctcc
      961 cgttctttcg atcagggaga gacctggagt gaagcgaaac gcatcaatca acatgcaggc
     1021 gattgcatcg acagcgacaa taccgtggaa ggtgccacct cagcagtggg gcctgacggc
     1081 gaattgtatg tggtgtgggc cggccctgac ggactgatct tcgatcgttc atacgatgaa
     1141 ggcgatacct ggctggatga ggatatcatc atcggcgagt ttcccggtgg atgggattat
     1201 gccatcccgg gattgttccg ttgcaacggg ctgcctattt tgaaatgcga taccagcggc
     1261 ggaccgaata acggcaccct ctacctcaac tggaccgatc agcggaacgg agaagaggat
     1321 accgatgtat ggctcgcaaa atcgaccgat ggtggtgaca cctggagtga tcctgtccgg
     1381 gtgaacgacg atgatgcggg taaacaacaa ttcatgacct ggatggatgt ggatcaggtg
     1441 aatggtcatc tcttctttgt gttctacgac agacgcaact atgacgacaa ccggaccgat
     1501 gtgtatgtag cctattcatt cgatggcgga gagaccttca ccaacgaacg catcagcgac
     1561 agccctttca caccgaacga aggcgtcttc ttcggcgact ataccaac
//
LOCUS       DAOMAX010000452         1605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_20932_length_1605_cov_4.381935, whole genome shotgun sequence.
ACCESSION   DAOMAX010000452 DAOMAX010000000
VERSION     DAOMAX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20932_length_1605_cov_4.381935"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1605
                     /locus_tag="PK742_13835"
     CDS             <1..>1605
                     /locus_tag="PK742_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HQU77721.1"
                     /translation="NLNAIFLLNVAAMNLGYYPDKVPSQWLIPVDRFGSEFSMEKFPN
                     IAIPLDVDVITLSGGVCTEDFNNDGYIDIFTSSWFLGDNVHYFQNNGDGTFTDRTKEA
                     NLEGITGGLNLIHADYNNDGFRDVFIPRGGWWNDYGKVPNSLLKNNGDGTFTDVTKSS
                     GLFSLYPTQTAVWSDFNNDGWLDLFVGNESRKNSEEYRCELFMSNGDGTFTNKAKEAG
                     ADLLAFSKGVAACDYDHDGLSDIAISSQGQPNILLHNESDGDQLRFRDVSGQAGISGP
                     VKSFPLTFLDYDNDGWEDLFIFTYDASNCDYDNAAEYLGKPVKGEYSVLYHNNGDGTF
                     SDISAGSGIQHAMTAMGFNYGDIDNDGFIDLYVGTGTPEYTSLVPNRMLRNNGGKGFQ
                     DVTHVGGFGHLQKGHGIGFADFDQDGDSDIYEDMGGGYEGDPFQSAFFENPGNGNTWT
                     CLQLEGVQCNRDAIGARVHVAVTMADGSKQIFHHLVGTGGSFGSNSLQLECGLGNAAK
                     IDSVTVDWPGGSSETFAEIPLKSCVQL"
BASE COUNT          434 a          371 c          414 g          386 t
ORIGIN      
        1 taacctcaat gccattttcc tcctgaatgt ggcggccatg aacctcggtt attatccgga
       61 caaggtaccc tcacagtggc tgatcccggt tgacagattt ggttcagaat tctctatgga
      121 gaagtttccc aatattgcca ttccgctcga tgtggatgtc atcaccctga gtggtggcgt
      181 gtgcaccgaa gacttcaaca acgacggata catcgacatc tttaccagca gctggttttt
      241 gggagataac gtgcattact ttcagaacaa cggcgatggc accttcaccg accggacgaa
      301 ggaagccaac ctggaaggta tcaccggagg gctcaatctt atccatgccg attacaacaa
      361 tgacggtttt cgcgatgtgt tcatcccgcg tggtggctgg tggaatgatt acggtaaagt
      421 acccaacagc ctgctgaaga acaatggcga tggcaccttc acagatgtga caaaaagcag
      481 cggattattc agtttgtatc ccacacaaac tgctgtgtgg tctgacttca ataacgacgg
      541 ttggctcgac ctctttgtag gaaatgaatc cagaaagaac agtgaagaat atcgctgtga
      601 actgttcatg agcaacggcg atggaacatt tacgaataag gcaaaagaag ccggtgcaga
      661 cctgctggct tttagtaaag gagtagctgc ctgcgactat gaccacgacg gactctctga
      721 tatagccata tccagccagg ggcaacccaa catcctcctg cacaacgaga gtgatggcga
      781 tcagctcagg ttcagagatg tatcaggaca ggccggaatc agcggacctg taaaaagctt
      841 tccgctcact tttctcgatt atgataacga tggatgggaa gacctcttca tcttcactta
      901 tgacgcatcc aactgtgact atgataacgc agcagaatat ctgggtaagc ctgtaaaagg
      961 tgagtattct gttctctacc acaacaacgg tgatggcacc ttctcagata tttcagccgg
     1021 gtcaggaatt cagcatgcca tgaccgctat gggattcaac tacggagaca ttgacaacga
     1081 tggtttcatt gatctctatg tgggtaccgg aactcctgaa tatacttcac tggtacccaa
     1141 cagaatgttg cgcaacaacg gtggtaaagg ctttcaggac gttacccatg tgggaggctt
     1201 cgggcatttg cagaaaggac atggcattgg attcgctgat tttgatcagg acggcgacag
     1261 cgatatttat gaagacatgg gaggtggtta tgaaggtgat cctttccaga gtgcattttt
     1321 tgaaaatccg ggtaacggaa acacctggac ctgtctgcaa ctggaaggtg tacaatgcaa
     1381 ccgcgatgcc atcggagcaa gggtacatgt ggcagtgacc atggcagacg gaagcaaaca
     1441 aatctttcac cacctggttg gtaccggcgg aagctttggc agcaacagcc tgcagctcga
     1501 atgtggtctt ggcaatgcag caaagatcga cagcgttaca gttgactggc ccggaggcag
     1561 ttcagaaact ttcgcagaaa ttcctttgaa gagttgtgtg cagct
//
LOCUS       DAOMAX010000453         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_21328_length_1589_cov_3.854628, whole genome shotgun sequence.
ACCESSION   DAOMAX010000453 DAOMAX010000000
VERSION     DAOMAX010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21328_length_1589_cov_3.854628"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PK742_13840"
     CDS             <1..238
                     /locus_tag="PK742_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQU77722.1"
                     /translation="GVKGGGCAGLSYILGFDSQKDNDQVYQFRDITILMEKTHGMYLV
                     GMEIDFVDGLENRGFSFNNPNAVQTCGCGTSFSS"
     gene            468..>1589
                     /locus_tag="PK742_13845"
     CDS             468..>1589
                     /locus_tag="PK742_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQU77723.1"
                     /translation="MSIPVSLNMNLRGIGQSATLAINERSKALRREGRKIYGLGLGQS
                     PFPIPQSVVDSLKMHAHEKDYLHVQGLPALRTAVAEFHIKKDLADIHPDQVLIGPGSK
                     ELMFLLQLVFYGEIILPSPSWVSYHPQAHILGRPVRFIHTSFEDKWRLTADMLERHCA
                     ADNDNTKPRLIILNYPSNPDGGSYTADELEAIGSIARKNNIILLSDEIYGQLHFEGEH
                     VSVSRYYPEGTIISSGLSKWCGAGGWRLGTFSFPPQLSWLREAMAAAASETYTSVSAP
                     IQYAAIRAFKCGINIERYLWRVRKILKALATDTVRILQDGGVRVHMPDGAFYLFIDCT
                     PFAEKLHARNIHNSMDMCEALLQETGVAILPGAAFNRPAN"
BASE COUNT          408 a          412 c          388 g          381 t
ORIGIN      
        1 aggtgtgaaa ggcggtggct gtgcaggttt atcctatata ttgggatttg acagccagaa
       61 agacaatgac caggtgtatc agtttcgtga tatcaccata ttaatggaga agacccatgg
      121 catgtacctg gtgggcatgg agatcgattt tgtggatggg ctggaaaacc gcggcttcag
      181 cttcaataac ccgaatgccg tccagacctg tggttgtgga accagtttct ccagctgagg
      241 atccggcatt tcagaaaaac aacggctgcc ttcgggcagc cttttttttg ggcaaaaaaa
      301 taccctgcca ttcagacagg gtacttacaa gtcaaccttt acccttagtt gtaacactta
      361 taccacatag actcaagagc atttaaaaaa gttgcaccag gacaaaattt ttttgataaa
      421 acggccaaaa tgagctgaaa aagcccgttt ttttttatct tttctctatg agtattcccg
      481 taagcctcaa tatgaacctg cgaggcatcg gccagtctgc caccctggcc atcaatgaac
      541 gttccaaagc cctgcgcagg gaaggcagga agatatacgg actcggttta gggcaatcgc
      601 cgtttcccat tcctcagagc gtagtggact ccctcaaaat gcatgcccat gaaaaagact
      661 acctgcacgt acagggtctt cccgctctgc gcaccgccgt ggccgaattt catatcaaga
      721 aagatctggc agacatccac ccggatcagg tactgatcgg cccgggcagc aaagaactga
      781 tgttcctcct ccagctggtg ttctatggcg agatcatcct gccatcacca tcctgggtat
      841 cttaccaccc ccaggcccat attctgggca gaccggttcg tttcatacat accagttttg
      901 aagataagtg gcgacttacc gccgatatgc tggaacggca ttgtgcggca gacaatgaca
      961 acaccaaacc caggcttatc atcctgaact atccgagcaa ccctgatggc ggaagctata
     1021 ctgcagatga actggaagcg ataggctcca tcgcccggaa gaataacatc atcctgctca
     1081 gtgatgagat ctacgggcaa ctccattttg aaggagaaca tgtatcggtt tccagatact
     1141 atcccgaagg caccatcatt tccagcggac tcagcaaatg gtgcggggca ggtggctggc
     1201 gactgggcac tttttctttc ccgccccaat tatcctggct ccgggaggct atggctgctg
     1261 cagccagtga gacctacact tctgtatcag ctccgatcca atatgctgcc ataagggcct
     1321 ttaaatgtgg tatcaatata gaacgatacc tctggcgggt gcgtaagatc ctgaaggcac
     1381 tggccactga tactgtgcgc attctgcagg acggtggtgt cagggtacat atgccggacg
     1441 gagcattcta cctgtttatt gactgcaccc ctttcgctga aaaactgcac gcccgaaata
     1501 tccataacag catggatatg tgcgaggcat tgttacagga aaccggcgtg gccattctgc
     1561 cgggtgcagc ctttaaccgg ccggccaat
//
LOCUS       DAOMAX010000454         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_21613_length_1578_cov_2.166120, whole genome shotgun sequence.
ACCESSION   DAOMAX010000454 DAOMAX010000000
VERSION     DAOMAX010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21613_length_1578_cov_2.166120"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="pepD"
                     /locus_tag="PK742_13850"
     CDS             complement(<1..930)
                     /gene="pepD"
                     /locus_tag="PK742_13850"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HQU77724.1"
                     /translation="MSVTSLEPTVLWKHFADLNAVPRPSKHEERVIQFMVDYGNSLGL
                     PTTKDAVGNVIIKKPASKGMENRQTVILQSHLDMVHQKNNDTVFDFSTEGIKMKINGD
                     WVEAEGTTLGADNGIGVSAIMAILESTDIPHPAIEALFTIDEETGMTGAMGLKGGLLD
                     GHILLNLDTEDDDELTIGCAGGIDITATATYEEATAPAGWTWFTMSVKGLTGGHSGMD
                     IHRGRGNANKLMNRLLAGLEKEHCIRIASIDGGGLRNAIPRESVAVFGAEAHRSDGVL
                     SFISGQSAILKQEYATTDPSIDVTIAETSAPSDV"
     gene            complement(1018..>1578)
                     /locus_tag="PK742_13855"
     CDS             complement(1018..>1578)
                     /locus_tag="PK742_13855"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77725.1"
                     /translation="VVFWLVIIGTTTLGTEISDFMDRSLHLGYTIGSLILVALLLLSL
                     LMWYLRFRDLRVYPIVDRTKELYFWIAVLFSNSLGTAFGDYLSDVLELSYLHGALITG
                     AIILVVVLLHYFTRLNEVLLFWIAFVFTRPFGATFGDFLTKPLAKGGLNLGTLPATLV
                     SVAVIGILIVIAHRQDDKALHEVPSS"
BASE COUNT          391 a          397 c          398 g          392 t
ORIGIN      
        1 cacatctgat ggtgcagaag tttctgcaat ggtcacatca atagatggat cagtagtagc
       61 atactcttgt ttcaaaatgg ctgactgccc ggagatgaaa cttagcactc catcagagcg
      121 atgtgcttct gcaccaaata cagctacact ctccctggga atggcattgc gcagtccgcc
      181 accgtcgatg gaagcaatgc ggatgcaatg ttctttctct agtcctgcaa gcagcctgtt
      241 catgagcttg ttggcatttc cgcggccgcg gtgaatgtcc atgccactgt gcccgccggt
      301 caatcctttt acgctcatgg tgaaccaggt ccatcctgcc ggggctgttg cttcttcata
      361 agttgctgtt gcggtgatat caatgcctcc tgcacagccg atggtgagtt cgtcatcatc
      421 ttcggtatcg agattcagga ggatgtgacc gtccagtagt ccgcctttca atcccattgc
      481 tccggtcata cctgtttctt cgtcaatggt gaacagggct tcgatggcag gatgcggaat
      541 atctgtggac tccagaatgg ccatgatggc agaaacgcct ataccgttgt ctgcaccgag
      601 tgtggtacct tcagcttcca cccagtcgcc gttgatcttc attttgatac cttctgtaga
      661 aaagtcaaac accgtatcgt tgttcttctg gtgcaccata tcaagatgac tctggagaat
      721 gaccgtttgt ctgttctcca tgcctttact cgccggcttt ttgatgatca cattacccac
      781 tgcatccttt gttgttggca gacccaggct gttgccatag tcaaccatga actgaataac
      841 cctttcttcg tgcttactcg ggcggggaac tgcattgaga tcagcaaaat gtttccagag
      901 cacggttggt tcaagagaag tgacagacat aatatagctt ttggctaaag atagggaatg
      961 ccctgcagga agactctcat ctgcagagat gatattggtc aaaagtgatt catacgatca
     1021 ggatgacggc acttcatgca atgccttatc gtcttgcctg tgtgcgatca cgatgagtat
     1081 tccgatgacc gccacagaga ccagcgtagc aggcagtgta cccagattca gtccgccttt
     1141 agccagcggc ttggtcagga aatcgccgaa ggtagcgccg aatggccggg tgaacacaaa
     1201 ggctatccag aacagcagca cttcgttgag tctggtgaag tagtgcagga gtaccactac
     1261 cagaatgata gcaccggtga tgagtgcacc gtgcaggtaa ctgagctcca gcacatcact
     1321 taaataatcg ccgaaggcag tacctaggct gttggagaac aggacagcaa tccagaaata
     1381 aagttccttg gttcgatcta cgatcgggta aacccgcagg tcccggaagc gcaggtacca
     1441 catcagaagc gaaagcaaaa gcaatgcaac caggatcagg ctgcctattg tataacccag
     1501 gtgcagggag cgatccataa agtcagagat ctcggttccc agtgtagtgg tgccgatgat
     1561 gaccagccag aatacaac
//
LOCUS       DAOMAX010000455         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_21743_length_1572_cov_3.266974, whole genome shotgun sequence.
ACCESSION   DAOMAX010000455 DAOMAX010000000
VERSION     DAOMAX010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21743_length_1572_cov_3.266974"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PK742_13860"
     CDS             <1..844
                     /locus_tag="PK742_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQU77726.1"
                     /translation="KLFLLNGEVQTTVYSFHLTATNAGDIIGHYDLVIDASDNFPTRY
                     LLNDACVLLDKPWIYGAVSAMEGQLAVLNFHSGPTYRCLFPTPPEPGAVPDCETSGIL
                     GTIPGTIGMLMATEALKILAGLPVVMQGTLLHWQATEQRFDKWTFRRDAEYKKHVPAT
                     LEAMRQTDYPAFCGIRDETSVSADDLVVQDRTYLFLDVRNTDELPEPWFDHLRWSLDE
                     IRSGINQPELQGDVLVYCQTGSRTPEAIRLLKQRYPDIHFVSLQGGIKAWQLAEMNKR
                     YGRT"
     gene            831..1271
                     /locus_tag="PK742_13865"
     CDS             831..1271
                     /locus_tag="PK742_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQU77727.1"
                     /translation="MAEHKRKTVFVQGAVSAQFVADSIANHSSKTDIGAHSIFLGQVR
                     RDEKDGKFVQAIDYTAHEEMANEQFHRIREDAFEKYPLTCMHIHHSLGAVAAGEISLF
                     VFTSSPHRRAAIDACNEIVERIKNEVPVWGKEVLEDGEYVWKTN"
BASE COUNT          434 a          386 c          408 g          344 t
ORIGIN      
        1 aaaacttttt ttgctgaatg gtgaggtgca gaccacggta tattcttttc atctgaccgc
       61 tacaaatgcc ggggacatca tcggccacta tgatctggtc atcgacgcca gtgacaactt
      121 ccccacacgc tatctcctga atgatgcctg tgtgctgctg gacaaaccct ggatatacgg
      181 agccgtttct gccatggaag gccagctggc tgtgttgaat tttcattcgg gtcctaccta
      241 tcgctgcctg ttccccaccc caccagaacc aggtgctgtt ccagattgtg agaccagcgg
      301 cattctgggc actatcccag gcaccatcgg catgctgatg gctaccgaag ctctgaagat
      361 cctggcggga ctaccagtgg tgatgcaggg gaccttattg cactggcagg caacagaaca
      421 acgcttcgac aagtggacct tcaggcggga tgcggaatac aaaaagcatg tgccggcaac
      481 cctggaagca atgcgtcaga cagactatcc tgctttctgc ggaatcaggg atgagacctc
      541 agtttccgcc gatgatctgg tcgtacaaga ccggacctat ctttttctcg atgtccgtaa
      601 taccgatgaa ctgccggaac cctggttcga tcatctcaga tggtcgctgg atgagatcag
      661 atccggtatc aaccaaccgg aattgcaggg cgatgtgctg gtgtattgcc agaccggcag
      721 tcgcacaccg gaagccatca gactattgaa gcagcgctat ccggatatac attttgtttc
      781 tttgcaggga ggcatcaaag cctggcaact ggcagaaatg aataaacggt atggcagaac
      841 ataaaaggaa aacagtattt gttcagggtg ctgtatcggc acagttcgtt gccgacagca
      901 tcgccaacca cagcagcaag actgacatcg gtgcacacag catttttctg ggacaggtgc
      961 gcagagacga gaaggatgga aaattcgtgc aggccattga ttatacggcg cacgaagaga
     1021 tggcgaatga acagtttcat cgcatacggg aagatgcctt tgaaaagtat ccgctcacct
     1081 gcatgcatat tcatcacagc ctgggtgctg tcgctgctgg cgagatcagc ttgttcgtgt
     1141 tcacttcttc accgcaccgg agagcagcca tagacgcctg caacgagatc gtggaacgca
     1201 tcaagaatga agtgccggtg tggggaaaag aggtgctgga agacggggag tatgtgtgga
     1261 agaccaacta gtagtgggta taggtattga gtattgagac cgataggttg gaatgctgaa
     1321 atacctttgc ggttgaatta agcagtgagc agtgagcagt gagtagtgaa aaataagccg
     1381 aagaacagca catataacta tcggaacaac caactaaaaa acaagatacc aactaccaac
     1441 aactaatcac caaataccaa taactaataa ccaacaacca accactcctc catgaacaac
     1501 atcacccata aatctccctc ctatcgcatg gcagtggcgc aggctgttgt cagtgtgagc
     1561 agggaagaga cc
//
LOCUS       DAOMAX010000456         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_21781_length_1571_cov_2.191953, whole genome shotgun sequence.
ACCESSION   DAOMAX010000456 DAOMAX010000000
VERSION     DAOMAX010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21781_length_1571_cov_2.191953"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..533
                     /locus_tag="PK742_13870"
     CDS             198..533
                     /locus_tag="PK742_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HQU77728.1"
                     /translation="MLLKDVIKELISFRDSRDWAQFHDSKNLATAISIEAAELNELFL
                     WKDVKESEEVDRERLKEELADVLAFAFLLAEKHGLDPLEIVLEKIKKNEEKYPVDKAK
                     GKATKYTEL"
     gene            530..>1571
                     /locus_tag="PK742_13875"
     CDS             530..>1571
                     /locus_tag="PK742_13875"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HQU77729.1"
                     /translation="MIVYRSTKSGFREDLDTGNIDGIIQEAFQRKLHRSTSAKELESW
                     WNSLHFMWDVISDTGIPENSGIAIECQIPQTSKRIDFIISGSDSTDRTDVVIVELKQW
                     TTAERTEKDGIVRTALGRGLHETAHPSYQAWSYAAMLEDFSETVYEENITLKPCAYLH
                     NYREDEVIRHSFYQTYLDKAPVFLKQDKQKLRAFIKTFIHSGDEGQTMYRIDQGRIRP
                     SKQLTEALVSMIRGNQNFILIDDQKLVYETALRLCEVAREGTKQVMIVEGGPGTGKSV
                     VAINLLVEITRRGMLTQYVTKNAAPRAVYQSKLTGTLSKTRFAALFQGSGRYVDAEKD
                     VFDALVVDEAHRL"
BASE COUNT          419 a          371 c          443 g          338 t
ORIGIN      
        1 aaagacagga agtacagcct acaacagtcg tttggctcaa tggcgggtga agtggttaac
       61 ttaatatact acctcgcatc aacttttgtg gtgtattgac agtttaatgc tccgaaattc
      121 gccactgcgc taagcgccaa atcgttgaat tggaattcat tcgttgaaaa aattatcttt
      181 tacagaagat cagttagatg ctattaaaag atgtaataaa agaattaatc tccttccgcg
      241 actctcgcga ctgggcccaa ttccatgatt ccaagaatct ggccacagcg atcagcatcg
      301 aggcagcgga attgaatgag ctgttcctgt ggaaggatgt caaggagtct gaagaggtgg
      361 atcgggagag gcttaaggag gaactggcgg atgtcctggc gtttgctttc ctgttggccg
      421 aaaagcatgg gcttgatccg ttagagattg tcctggagaa gatcaagaag aacgaagaga
      481 agtacccggt agacaaggcc aagggaaagg ccaccaaata caccgagcta tgatcgtcta
      541 tcggtccacc aagtcaggat ttcgggagga cctggataca gggaatatcg atggcatcat
      601 tcaggaggca tttcagcgga aactgcaccg gtcgacgtct gcaaaggaac tggaatcatg
      661 gtggaactcc ctgcacttca tgtgggatgt gatctccgat acagggatac ctgaaaactc
      721 cggcatagct atagaatgcc agatccccca gacctccaag cggatcgact ttatcatatc
      781 gggatcggat agtacagatc gcacggatgt agtaatcgtc gaactcaagc aatggacaac
      841 agcggaacga acggagaagg atgggattgt ccggaccgct ctgggtagag ggctgcatga
      901 aacggcccac ccgtcctacc aggcatggtc ctatgccgcc atgctggagg atttcagtga
      961 aacggtctac gaagagaaca tcaccctgaa gccctgtgcc tatctccaca actacaggga
     1021 ggatgaggtg atccgccatt cattctatca gacctacctg gacaaggcgc ctgtcttcct
     1081 gaagcaggac aagcagaagc tcagagcctt tatcaagact tttatccatt ctggtgatga
     1141 ggggcagact atgtatcgca tcgaccaggg caggatccgg ccgtcgaaac aactgacgga
     1201 agccctggtc tccatgatcc ggggcaacca gaacttcata ctgatcgatg accagaagct
     1261 ggtgtatgaa acagccctcc gactgtgtga ggtggcccgg gagggaacca agcaggtgat
     1321 gatcgtggag ggaggtcctg gtaccggaaa gtccgtggtg gccatcaacc tgctggtcga
     1381 gatcacccgg cggggcatgc tcacccagta tgtcaccaag aatgccgctc caagggcggt
     1441 gtatcagagc aaattgacag gtacgctgtc gaagacccgc tttgctgccc tctttcaggg
     1501 ttcgggtcgg tatgtggacg cggagaagga tgtgttcgat gccctggtgg tggatgaagc
     1561 gcaccgactc a
//
LOCUS       DAOMAX010000457         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_22868_length_1530_cov_3.884068, whole genome shotgun sequence.
ACCESSION   DAOMAX010000457 DAOMAX010000000
VERSION     DAOMAX010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22868_length_1530_cov_3.884068"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1222
                     /locus_tag="PK742_13880"
     CDS             167..1222
                     /locus_tag="PK742_13880"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQU77730.1"
                     /translation="MNVAVWMWISVLFSCGGLDDGGKRSVSEELLPLTGKLPLPENGF
                     SNAYADLDGTMWFCSNGGGVFHLDGESFMHYTVKDGLSSDQVFSVISDHHNNLWFGTQ
                     NGLTKYDRTELEQIPLPFRDTSGVWLDQVYPTISPNAVHSLAADDLGNLWIGTAGGGA
                     YRYDGKEFRSFLAETGRKQEDSLYHNWIPYIEKDNNGYLWFASMTYGGVTCYDGTEFR
                     QFLIEDGLTDNQVRTIYCDRSGNIWIGFNGNRNSGLTMYDGTSFKSYYVEDGLCNKRI
                     RAIFEDREGTIWLGTGLGNVCVFDGQQFSEFNYEGKTFSDVLFIIGDQEDNIWFGGRY
                     GIWKYDGEILTEIATNE"
     gene            1321..>1530
                     /locus_tag="PK742_13885"
     CDS             1321..>1530
                     /locus_tag="PK742_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77731.1"
                     /translation="MRSVAFFFFLVAVKGLAFSQSYPTYGPEIPVTVAGLTFDAMEPF
                     LSPDGNTLFFNSLNAGGNTNLYQAER"
BASE COUNT          434 a          298 c          386 g          412 t
ORIGIN      
        1 ttttgcctca attggcggtg atgtgcaaaa ttgaagttga atgctcctat caaacttttg
       61 tgcaggttga cagtgaagta ctccgaaatc gcccactgcg gcaacacgca aaacgctacc
      121 aatccctaaa tagcaaatct tgatcagaag caagataaga gaaagtatga atgtcgcagt
      181 atggatgtgg atctcggtat tgttttcctg cggtgggctg gatgatggtg ggaagcggtc
      241 agtttcggaa gaacttttac cgctaacagg caaactgccg cttcctgaga atggattttc
      301 aaatgcttat gctgatctgg atggaaccat gtggttctgc agcaacggtg gtggtgtatt
      361 tcatctggat ggtgagtcat ttatgcatta taccgtaaaa gacggtttaa gcagtgatca
      421 ggtcttttct gttatatcag atcaccataa taacctctgg tttggaaccc agaacggttt
      481 gacgaagtat gaccgaacag agttggaaca aattccgctt ccattcagag atacatctgg
      541 tgtctggctg gaccaggtct atccaaccat tagccccaat gcggtgcatt ctttagctgc
      601 tgatgatttg ggtaacctgt ggatcggcac agccggtggc ggagcatata ggtatgatgg
      661 aaaagaattc agatcatttt tagctgaaac cggaagaaaa caggaagaca gcctctacca
      721 taactggata ccttatatcg aaaaagataa caacgggtat ttgtggtttg cgtctatgac
      781 ctatggcgga gtgacatgct atgacggcac tgaattcaga caatttttaa tagaagacgg
      841 cctgacagat aaccaggtgc gtaccatcta ttgcgacaga tcaggaaaca tttggatagg
      901 attcaatgga aacagaaaca gcgggttgac catgtatgat ggtacatcat tcaaaagcta
      961 ctatgtggaa gacggactct gcaataaacg catacgtgca atatttgaag accgggaagg
     1021 aaccatctgg ctgggtacag gtttgggaaa tgtatgtgta tttgatggtc agcagttctc
     1081 tgagtttaac tatgagggga agactttttc agatgtattg ttcattattg gcgatcagga
     1141 agataacatc tggtttggtg gcagatatgg tatatggaag tatgatgggg agatcctgac
     1201 cgaaatagca acaaatgaat aagaaagcct gcagccggca atgaatatca gagtcaatgg
     1261 cataaaatct gcaggacaat aaccaggagc ctaagccctt tatttaaaca gttcagacca
     1321 atgagatcag tagcgttttt cttcttcctg gtggccgtga aagggttggc tttttctcaa
     1381 agctacccga cctacggtcc tgagatcccg gtcactgttg ccggtctgac gtttgatgcc
     1441 atggaacctt tcctgtcgcc tgacggtaat accctgttct ttaactcgct taatgccggt
     1501 ggcaatacca atctgtacca ggctgaaagg
//
LOCUS       DAOMAX010000458         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_22966_length_1526_cov_2.927940, whole genome shotgun sequence.
ACCESSION   DAOMAX010000458 DAOMAX010000000
VERSION     DAOMAX010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22966_length_1526_cov_2.927940"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PK742_13890"
     CDS             complement(<1..783)
                     /locus_tag="PK742_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQU77732.1"
                     /translation="MTLISSISGIRGTIGGRPGDNLTPADIIRFTTAFGRWVIEKTGN
                     RVVVVGRDARISGEMVNRLVCGTLQSIGINVVDLGLSTTPTVEIAVPLEKAGGGIILT
                     ASHNPKHWNALKLLNDKGEFLSAADGAEVLALTDSDMEFSTVDEIGEYQTRNHYIRTH
                     IDQILALDMVDVKAIRDRRFRIVIDCVNSTGGISVPELLHALGVTDLVELFCAPNGRF
                     PHNPEPLPENLGTIAQEVKLQKADLGIVVDPDVDRLALVCEDG"
     gene            901..1476
                     /locus_tag="PK742_13895"
     CDS             901..1476
                     /locus_tag="PK742_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQU77733.1"
                     /translation="MIFPEWLEQVDRAGFLLIHRTWKNAFFDSLMPLLRNRDTWVPFY
                     VLLAGWLLVRMKWKGLLLIVIIAAVTGLADLVSSSVLKPVIGRDRPCHEAQLEGEVFP
                     MVHCGNNGSFTSSHAANHMALAAAISMLLLRRRKWLAALLYFWAFLIGYAQIYVGVHY
                     PTDVLGGFLLGFLLAWLAVRIAFRSGLQVPA"
BASE COUNT          325 a          387 c          424 g          390 t
ORIGIN      
        1 accgtcttcg cacaccagtg ccagtcggtc cacatcagga tccaccacga tgcccagatc
       61 agctttctga agctttactt cctgtgcaat ggtgccgagg ttttctggaa gtggttcagg
      121 attgtggggg aaacgaccat tcggagcgca gaatagttcc accagatcgg tcaccccgag
      181 ggcatgcaac agctccggga cactgatgcc accggtcgaa ttcacacagt cgatgacgat
      241 gcggaatctg cggtcgcgga tggctttcac atcgaccata tccagtgcca ggatctgatc
      301 gatatgggtg cggatataat gatttcttgt ctggtattct cctatctcat cgacggtgct
      361 gaactccata tcgctgtcgg tcagcgccag tacttcggca ccatcggcag cactcagaaa
      421 ttcgcctttg tcgttcagca gcttgagagc attccagtgt ttcgggttgt ggctggcagt
      481 gaggatgatg ccaccaccgg ctttttccag cggaacggct atctctacag taggggtggt
      541 actcaggccg aggtccacca cattgatacc gatactttgc agggtgccgc aaacaagacg
      601 attcaccatc tcaccgctga tacgtgcatc tcttcccacc accaccacac gattgcctgt
      661 tttttcaatg acccagcgcc cgaaagctgt ggtaaaccgt atgatatctg ccggagttag
      721 attatcgccc ggcctgcctc caatggtccc tctgatgccg gatatactgc tgatgagcgt
      781 cacgctgaaa attttggcaa agttacgaaa tcgggagagg ggtgtccgac actccggggt
      841 gagtcctcaa ttctgggggg tggcatagcc ggggggtgta ttgattaact ttgcatcctg
      901 atgatctttc ctgaatggct ggagcaggtg gacagagcag gatttctgct catccacaga
      961 acgtggaaga atgccttctt cgattcgctg atgcctctgc tgcgaaacag ggatacctgg
     1021 gtgccatttt atgtgctgct ggctggatgg ttgctggtgc gtatgaagtg gaaagggctg
     1081 ttgcttattg tgatcatagc agcagtaaca ggtctcgctg acctggtaag cagttctgta
     1141 ctgaaacctg tgataggacg tgatcgtccc tgtcatgaag cacagctgga aggggaagtt
     1201 tttcctatgg tgcactgcgg taacaatggc agcttcacca gctcgcacgc cgccaaccat
     1261 atggcactgg cggcagccat ttctatgttg ctgctacgca ggcggaaatg gctggcagct
     1321 ttactgtatt tttgggcctt cctgatcggc tatgcgcaga tctatgtagg ggttcactat
     1381 ccgaccgatg tattgggagg gttcctgctc ggattcctgt tagcctggct ggcggtcagg
     1441 atagctttca gatcaggatt acaagtacct gcatgagtgc aacagaaacc atattgctga
     1501 tcctgatggc tgcggccgga gccttt
//
LOCUS       DAOMAX010000459         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_23306_length_1514_cov_4.644962, whole genome shotgun sequence.
ACCESSION   DAOMAX010000459 DAOMAX010000000
VERSION     DAOMAX010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23306_length_1514_cov_4.644962"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK742_13900"
     CDS             complement(<1..483)
                     /locus_tag="PK742_13900"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQU77734.1"
                     /translation="MAMISTLRKHSWIVLVFIALSLIGFLLMDAFNSNSGLVKNRRQT
                     FAEIDGSEITPAQLDAKYSEVLTQYLTQTQQLLNYQTGAFTLDNQTEFQLREQAWNDL
                     LQTNIMNRQLEAAGLHVTEQEKTDLIYGPDPHPYIKNYYVGLSGSGKYDPAAFNQYIT
                     F"
     gene            complement(563..808)
                     /locus_tag="PK742_13905"
     CDS             complement(563..808)
                     /locus_tag="PK742_13905"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQU77735.1"
                     /translation="MEQDWMMVYNTADRQQAWIVRSALEEQADIRVVAVDKTSSPYGF
                     ALPGEVELYVHTSQAEKAIELIYAMETAGDSDSRTEF"
     gene            complement(780..>1514)
                     /locus_tag="PK742_13910"
     CDS             complement(780..>1514)
                     /locus_tag="PK742_13910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQU77736.1"
                     /translation="HALEVYQFCTSLGRFGLLTFAFLWLIGESAGSFLQLRKSPSLQQ
                     LIWVFPLMISAAVVIGAVNEWNQGLNLPEAWSGLEKQMRQMEEQAKGMTDAFLGTTTI
                     GGLLINIFLIGIVAAVTEELFFRGLVQGIFHRWTGKAHAGIWIAAFLFSFIHMQFYGF
                     FPRLLLGALLGYICYWSGSLWTAMAGHFINNTAAVILYFLVSNGSVEQDPTETGTWAQ
                     ALVFLPVLAIFIWQFRRHGARLDDGV"
BASE COUNT          366 a          403 c          379 g          366 t
ORIGIN      
        1 gaaggtgata tactggttga aggcagcagg atcatatttt ccactgccgc tcagaccaac
       61 atagtagttc ttgatatatg ggtgcggatc cggtccgtag atcagatcgg tcttctcctg
      121 ctcggtcaca tgcaaccctg ctgcctccag ctgacggttc atgatgttgg tttgcagaag
      181 atcattccag gcctgctcac gcaactggaa ttctgtctga ttgtccaggg tgaaggctcc
      241 tgtctggtaa ttcagcaact gctgggtctg ggtcaggtat tgtgtgagca cctcactgta
      301 tttggcatcc agctgggcag gggtgatctc tgaaccatca atttctgcaa acgtttgacg
      361 acggttcttc accagcccgg aattcgagtt gaatgcgtcc atgagcagga agccgatcag
      421 cgacagggcg ataaacacca gaacgatcca ggaatgcttc cgcaaagttg atatcatagc
      481 cataataaac tgctactttt tactattgag cctgcaaatt taggggaatt ctgatggatt
      541 ttgccagcag acgccgattt gcctaaaatt cggtccggct gtcgctatct ccggcagttt
      601 ccatcgcata gattagttca atggctttct ctgcctgact ggtatgtaca tacagctcca
      661 cttcacccgg caaggcaaag ccataagggc tgctggtctt gtcaactgcc actaccctga
      721 tgtctgcctg ttcttccaaa gcagagcgca cgatccaggc ctgctgccgg tctgccgtat
      781 tatacaccat catccagtct tgctccatgg cggcggaatt gccatataaa tatggccaaa
      841 acaggcagaa ataccaaggc ttgcgcccag gttccggtct cagtgggatc ctgttccacg
      901 gaaccattac tgaccagaaa gtacaggatg accgctgctg tattgtttat gaaatgcccg
      961 gccatggcgg tccacaggct gccgctccag taacagatat agcccagcag tgctcccagc
     1021 aggagccggg ggaagaagcc gtagaactgc atgtgtataa aagagaaaag aaaggcagct
     1081 atccagatac cggcatgtgc tttgcctgtc cacctgtgga agatgccttg tacgaggccg
     1141 cggaagaaca gctcttctgt taccgcggcg acaatgccga tcaggaatat attgatcagc
     1201 aatccgccaa tggtagtggt tcctaagaac gcatctgtca tgcctttggc ctgctcctcc
     1261 atctgccgca tctgcttttc cagtccgctc caggcttctg gcagattcag tccctggttc
     1321 cattcattga ccgctcctat gaccaccgca gcactgatca ttagcggaaa tacccagatc
     1381 agctgttgca gggaaggact ctttctcagt tggagaaagg atcctgccga ctctccaata
     1441 agccacaaaa aagcaaaagt gagcaggccg aaacgcccca ggctggtgca gaactgatag
     1501 acctccaggg catg
//
LOCUS       DAOMAX010000460         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_23347_length_1513_cov_4.214678, whole genome shotgun sequence.
ACCESSION   DAOMAX010000460 DAOMAX010000000
VERSION     DAOMAX010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23347_length_1513_cov_4.214678"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK742_13915"
     CDS             <1..388
                     /locus_tag="PK742_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HQU77737.1"
                     /translation="EQLHAAMEMIDRNGSGVLLYMQQEGRGIGLLNKLKAYRLQEDGR
                     DTVEANLDLGLPMDQRDYGVGAQILRDLGIERLRLITNNPKKRVALKGYGLEIVENVK
                     IEMVPNPHNERYLLTKRDKMGHDILR"
     gene            complement(396..779)
                     /locus_tag="PK742_13920"
     CDS             complement(396..779)
                     /locus_tag="PK742_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77738.1"
                     /translation="MLNPLKSTLRTSFPEKDGWKLYNRYNWSSRVHDYVLQKESGDST
                     LRIVVEINFGDQVLTDDFNKFAALSRRLESGKFKVIRTVMVIQENSRIEGQPFEGLEI
                     MNMAQLIQNGVMGLKQTLSNSKLVA"
     gene            complement(1032..1181)
                     /locus_tag="PK742_13925"
     CDS             complement(1032..1181)
                     /locus_tag="PK742_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77739.1"
                     /translation="MAILLTILMSLGLVSNSNVTINNTAVNQKDHQHMGGQYNSGNWE
                     WDNTH"
BASE COUNT          424 a          296 c          369 g          424 t
ORIGIN      
        1 cgaacaactg catgcggcca tggaaatgat agaccgcaat ggcagtgggg tacttttata
       61 tatgcagcag gaaggccgcg gaatcgggct cctgaataaa ctgaaagcct acaggctgca
      121 ggaggatggt cgtgatacgg tagaggctaa tctggatctc gggctgccga tggatcagcg
      181 ggactatggc gtaggagcac agattttgag agacctgggc attgaacgcc tcaggttgat
      241 caccaataat cctaaaaagc gggtggcact aaaaggatat ggtctggaga tcgtagagaa
      301 tgtaaagatc gaaatggtac caaacccgca taacgaaaga tatctgctga ccaaacggga
      361 taaaatgggt cacgatatcc tcaggtgatc tctgttcagg ctaccagttt tgagttagat
      421 aaagtctgtt tgagccccat gacaccgttc tggataagct gggccatatt catgatctcg
      481 agtccttcaa atggctggcc ttctatccgg ctgttttcct gaatgaccat gaccgttctg
      541 atcaccttga actttcctga ttccagcctg cgtgacaggg cggcaaattt gttgaaatca
      601 tcggtaagca cctggtctcc aaagtttatt tctaccacaa tgcgaagggt ggagtcgcca
      661 ctttccttct gcagtacgta gtcatgaacc ctggaggacc agttgtaccg attatacagc
      721 ttccacccat ctttttcggg gaagctggtg cgcagagtag acttgagcgg gttaagcata
      781 tctacttgtt ttttcgtttg ggtgtgtgtt gcatgatgca tggtagtccc gttatggggg
      841 ctaaattctg acccttttaa agggagaaca aggaagaaaa cggggatagc tcactcgaat
      901 ttaccgagca agcaatacat taactatttg ataatcaatt gattaccaat ccagaactgg
      961 acagtaattc acatatacac cagctgcttt tgcagctgtg tttccatctg gttgatgggt
     1021 ttacaacagg atcaatgggt gttatcccat tcccagttac cggagttgta ctggcctccc
     1081 atgtgttgat ggtctttttg gttgactgct gtgttattga tggttacatt tgagttgcta
     1141 accagaccca gagacatcag aatagttaat aagattgcca tagttgtaag tttatcagat
     1201 agttaagtaa tgtgacaccg aatctatgtt ctttgtcaga aaaaagcaag tttttaaaag
     1261 gtaaaataat ttcaactgtc aatgatgata tataataaaa catgtgtgcg attgaggatt
     1321 ttcaaatgta attaggaaat ctttgaatat aattacattg ctttggagaa aattacgcag
     1381 ttgataagta ccctgaccac cagagaggtc aatgccatca gaaagtttta ccggttgcgg
     1441 cctcaggctg atgccaatca gcgtttcagg ctatttgaaa aggtgttaaa cgatcctgaa
     1501 ttaagtgaca tgg
//
LOCUS       DAOMAX010000461         1490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_24041_length_1490_cov_4.168641, whole genome shotgun sequence.
ACCESSION   DAOMAX010000461 DAOMAX010000000
VERSION     DAOMAX010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24041_length_1490_cov_4.168641"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PK742_13930"
     CDS             complement(<1..239)
                     /locus_tag="PK742_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HQU77740.1"
                     /translation="MKKKVLFIINPIAGVKRKDKLPRLIDAALDHVQYTYDIVYTEHR
                     GHATEIAERAIKDGVEVVAVAGGDGSVNEVAMALRG"
     gene            complement(236..>1490)
                     /locus_tag="PK742_13935"
     CDS             complement(236..>1490)
                     /locus_tag="PK742_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HQU77741.1"
                     /translation="GELVINTPFSVIYAFGHRKLVEPNFYTPEELQQLYSPYHDQVDF
                     YHTDTPGNVCVIVLESFSKEYIGYFTGNKGYTPFLDSLLGQGLAFTNFYANGKSSNQG
                     IIATVSGIPVMMEEPFISSLYKNNAFVGIGSLLQTKGYYSAFFHGANNGSMGFDAFTQ
                     RAGFNAYYGRNEFGDDTYFDGNWGIFDEPFLQYTAKTMSTLPEPFYSQIFTISSHHPY
                     TMPQQYIGKFPKGPIPMCEVVGYSDMALRKFFEEARKQPWFENTLFILTADHNGPPTP
                     GNDFYKNQVGAHSTWMLMYKPDGSFSGTTNMTAQQTDIMPTVLDYVDYDGPYCAFGTS
                     VFDTITTRFAFDYHAGDYLLVHEDTALIYNGFDRELYYFPNDSTLSNDIQQHAPASLD
                     YMDTRLRAIIQVHHKAMLHNQLTAQ"
BASE COUNT          369 a          366 c          378 g          377 t
ORIGIN      
        1 ccgcggagag ccatggccac ttcattgacc gaaccatcac caccggcaac tgccaccacc
       61 tccacaccat cttttatcgc cctttcagcg atctctgtgg catgaccacg atgttccgta
      121 tagacgatgt cataggtata ttgcacatga tccagagcag catcgatcag acgaggcagc
      181 ttgtccttgc gcttcactcc tgcaatggga ttgataatga acaggacctt tttcttcact
      241 gggctgtcag ctggttgtgt agcatagctt tatggtggac ctgaatgatg gcccgcagcc
      301 gggtgtccat gtaatctaag gaagccggag catgttgctg tatgtcgttg gacagagtac
      361 tgtcgttcgg aaaatagtaa agctctctgt caaaaccatt atagataagc gctgtgtctt
      421 cgtgcaccag caggtaatcg ccggcgtggt aatcaaatgc gaagcgggtt gtaatggtgt
      481 caaagactga ggtaccaaat gcacaatacg gaccatcata atccacataa tccagcaccg
      541 ttggcatgat gtcggtctgc tgtgcggtca tgttggtggt accgctgaaa ctgccgtcgg
      601 gtttgtacat cagcatccag gtactgtggg cgcccacctg gttcttgtag aaatcatttc
      661 ccggtgtagg cggaccatta tgatcggctg tcaggatgaa aagcgtattc tcgaaccacg
      721 gttgcttccg ggcttcctca aagaattttc gcaatgccat atcgctgtac ccaactactt
      781 cgcacatcgg aatcggaccc tttggaaact taccgatata ctgctgcggc atggtatagg
      841 gatggtggga tgagatggtg aagatctgtg aatagaaagg ttccggcagg gtgctcatgg
      901 tctttgccgt gtattggagg aagggttcgt caaagatgcc ccagttaccg tcgaaatagg
      961 tgtcatcgcc aaactcattg cgaccataat acgcattgaa acccgcccgc tgggtgaagg
     1021 catcaaagcc catactgccg ttgttggcac cgtggaagaa agcgctgtaa tatcctttgg
     1081 tctgcagaag actgcctata cccacaaatg cattgttctt atacaggctg gagatgaatg
     1141 gctcttccat catgaccgga atgccactta ccgtagcgat gatgccctgg ttgctcgact
     1201 tgccgttcgc ataaaaattg gtgaacgcaa gcccctgacc caacagcgaa tccaggaaag
     1261 gtgtatagcc tttattgccg gtgaagtagc ctatatattc cttactgaaa ctctcgagca
     1321 cgatgacaca tacattgccc ggtgtatcgg tatgatagaa atctacctgg tcgtgataag
     1381 gcgaatacag ttgctgcaat tcttccggcg tataaaaatt cggctccacc agctttcggt
     1441 gcccgaatgc ataaatgacg gagaaaggtg tattgatgac cagttccccc
//
LOCUS       DAOMAX010000462         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_24346_length_1479_cov_3.940309, whole genome shotgun sequence.
ACCESSION   DAOMAX010000462 DAOMAX010000000
VERSION     DAOMAX010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24346_length_1479_cov_3.940309"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..287)
                     /locus_tag="PK742_13940"
     CDS             complement(105..287)
                     /locus_tag="PK742_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77742.1"
                     /translation="MNKLNQFRLLVVIIIVAPAMHYFIAGKDQEHTLIRNILVIVQIG
                     AGLLLLLLYGRKGNRT"
     gene            complement(447..>1479)
                     /locus_tag="PK742_13945"
     CDS             complement(447..>1479)
                     /locus_tag="PK742_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77743.1"
                     /translation="TFDAMEPFLSPDGNTLFFNSLNAGGNTNLYQAERVNDTTFTFGG
                     LVNGTYDPAPGHLDAVASLDSDGHFFWVSLRSIPNLYTGNYLSGNVADISPVYGTCNV
                     LTPGWIIMDAAIDPGGDLLFYSNGYFGPTYTECVGVPCEAMLGIAQKVNDSTFDKTAY
                     SDAVLENVNDTAYLVYAPQVSGDGLELYFTRLLKTGFDTEICVAVRSTVADAFSEPAV
                     LYSNYGFFPEAATLSNDKQRLYYHQKNEAGVYAVYMRYREPATYAEESGNNISWALYP
                     NPAKDVLQVALPDASAPFIIVIRSAAGQEVWKVSNSSSINISHLASGRYYLTVYQHGR
                     TGTKEFIID"
BASE COUNT          393 a          369 c          355 g          362 t
ORIGIN      
        1 ggaggattga tttcataggg atgaaaataa cagaataaac cgaaagtgtc tcctgcggct
       61 gtcagagtaa aaagaagggt attcctgcca caccccggca gcattcatgt acggtttccc
      121 tttctgccat acagcagcaa caggagcagg cctgcaccta tctgtacgat gaccagtata
      181 tttctgatca gtgtatgttc ctggtctttg cccgcaatga aatagtgcat ggccggtgcc
      241 acgatgataa tgaccacgag caggcgaaat tggtttaatt tattcattgc ctcgtatttt
      301 gattaacagc gagatcgcat actgtcatac tcatgataaa tataatgcaa gcagatcatt
      361 gcgtacgtct aatcttctat gtggctgcag cctctgatcc gttttcagat tctcacatca
      421 gattggccgg atgccgacac caggcactag tctattatga actctttggt accagtccgg
      481 ccatgctgat acactgtcag gtagtatcgt ccgctggcca ggtgagagat gttgatcgat
      541 gaactgttgg agaccttcca tacctcttgc ccagcggctg atcgaatcac gatgataaag
      601 ggtgctgagg catcagggag cgccacctga agcacatctt tagccgggtt tggatacagc
      661 gcccaggaaa tattgttacc tgattcttcg gcataggtag caggctctct gtatcgcata
      721 tagacagcat atacacctgc ttcgttcttc tggtggtaat acagtctttg tttatcgttt
      781 gacaaggtag cagcttccgg aaaaaacccg tagttggaat acaacacggc aggttcacta
      841 aaggcatcag ccacagtact ccgcactgca acacagatct ctgtatcaaa accggttttc
      901 agcaaccttg tgaaatagag ctccagtccg tcgccggaca cctgcggggc atacaccaga
      961 taagccgtgt catttacatt ttccagtacg gcatcgctgt aggcggtctt gtcaaaagta
     1021 ctgtcattga ccttttgagc gattcccagc atggcttcac agggcacccc cacacattcg
     1081 gtataggtag ggccgaaata cccgttgctg taaaagagca ggtctccgcc gggatcaatg
     1141 gcagcatcca tgatgatcca gccgggagtc aggacattgc atgtaccata caccggcgaa
     1201 atatccgcta cattgcccga caagtaattg ccggtataaa ggttggggat actgcgcagc
     1261 gaaacccaga agaagtgccc gtcactatcc aaagacgcaa ccgcatccag gtgcccgggt
     1321 gcaggatcat aggtaccatt caccaaacca ccgaatgtaa aagtggtatc attcaccctt
     1381 tcagcctggt acagattggt attgccaccg gcattcagcg agttaaagaa cagggtatta
     1441 ccgtcaggcg agagaaaagg ttccatggca tcaaacgtc
//
LOCUS       DAOMAX010000463         1466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_24727_length_1466_cov_4.406804, whole genome shotgun sequence.
ACCESSION   DAOMAX010000463 DAOMAX010000000
VERSION     DAOMAX010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24727_length_1466_cov_4.406804"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK742_13950"
     CDS             complement(<1..227)
                     /locus_tag="PK742_13950"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627822.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HQU77744.1"
                     /translation="MNKQVKAFMQSVADRNPNEPEFLQAVLEVAEVIVPFTSNYPDIQ
                     KSRILERITEPERVIIFRVPWVDDKGEVQINR"
     gene            complement(358..795)
                     /locus_tag="PK742_13955"
     CDS             complement(358..795)
                     /locus_tag="PK742_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HQU77745.1"
                     /translation="MQLHFSNYSIRTRSGEKEEVWDIIRRRWVTLTPEEWVRQHLLHY
                     FVHDLHFPAGRVAVEKQVVVNGLKKRTDILLFDQEARPLLLVECKSPDVPLTDQTFRQ
                     AAIYNLEWKVPFMMISNGHSHYCAAIDHEAQSFTMLEGLPELA"
     gene            831..>1466
                     /gene="holA"
                     /locus_tag="PK742_13960"
     CDS             831..>1466
                     /gene="holA"
                     /locus_tag="PK742_13960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQU77746.1"
                     /translation="MTINDLYKGLKARQFVPVYLLQGDEPFFIDRAVRFFETEILPEA
                     ERSFNLTIFYGKDSKAQDIIDTCMRYPMFSEHQVVIIKEANQLRDLEKLESYFEHIVP
                     TTILVIAYKNGSYDKRRKVYKLISQKGVVFDSNKIYDRELPAFVDRLLKEKKLTIDDK
                     ASRMLVDSIGNNLAAIVNEIDKISLHLQAGEKVTADHIEKFVGISKEFNVFE"
BASE COUNT          379 a          363 c          352 g          372 t
ORIGIN      
        1 cgattgatct gtacttcccc cttgtcgtcc acccagggaa cccggaaaat gatgacacgc
       61 tcaggttcag tgatcctctc caggatgcgt gacttttgta tatcaggata gttcgatgta
      121 aagggaacga tgacttcggc tacttccagc actgcctgca gaaattccgg ttcgttgggg
      181 ttgcgatcag ccacagattg catgaacgct ttgacctgtt tgttcataag gagcgatttt
      241 gtcagtagcg aatgtagcaa ttatttgaaa cgggcaaatt gacttatatc cctgtttttc
      301 atgttgaaaa tcccctttca tccggccggc taagggaatt atttgatggg cacgctgtta
      361 cgccagttca ggcaggccct ccagcatagt aaaactttgt gcctcgtgat caatggctgc
      421 acagtaatga gaatggccat tggatatcat catgaagggc actttccatt ccaggttgta
      481 gatggctgcc tgacgaaagg tctggtctgt aagcggcaca tcgggcgatt tacattccac
      541 cagcaacaat ggtctggctt cctgatcgaa cagaaggata tcagtgcgtt ttttcaatcc
      601 gtttaccacc acctgcttct ctactgccac tcttccggct gggaagtgca ggtcatgcac
      661 gaaataatgc agcagatgct gacgaaccca ttcctcagga gtaagtgtca cccatctgcg
      721 tctgatgatg tcccatacct cttctttttc gccactgcgt gttcggatgg aatagttgga
      781 aaaatgcagt tgcatgaacc tgcgaatttc ggtaaaatta ccggctgttc atgaccatca
      841 acgacctcta taaaggactc aaggcacgcc agttcgtgcc cgtatatctg ctgcagggcg
      901 atgaaccctt cttcatcgac cgggccgttc gttttttcga gacggagatc ctgccggaag
      961 ccgaacgctc ttttaatctg accatctttt acggaaagga cagtaaagcg caggatatca
     1021 tagatacctg tatgcgctac cccatgttct cagaacacca ggtggtcatc atcaaagaag
     1081 cgaaccaatt gcgtgacctc gagaaactgg aatcttattt cgagcacatc gtacccacta
     1141 cgatcctggt catcgcctac aagaacggct cctatgacaa gcgcagaaag gtctataagc
     1201 tcatcagcca gaagggtgtg gtgttcgatt cgaataagat ctatgaccgc gaacttcccg
     1261 ctttcgttga cagactgctg aaagagaaga aactgaccat cgacgacaag gccagccgca
     1321 tgctggtgga cagcatcgga aataacctgg ctgccatcgt caatgagatc gataagatca
     1381 gtctgcacct gcaggcggga gaaaaggtca ctgccgatca cattgaaaaa ttcgtaggca
     1441 tcagcaagga gttcaatgtg ttcgaa
//
LOCUS       DAOMAX010000464         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_25230_length_1450_cov_4.110394, whole genome shotgun sequence.
ACCESSION   DAOMAX010000464 DAOMAX010000000
VERSION     DAOMAX010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25230_length_1450_cov_4.110394"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..519)
                     /locus_tag="PK742_13965"
     CDS             complement(94..519)
                     /locus_tag="PK742_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HQU77747.1"
                     /translation="MSIWFKKDLQPADLMTLNPGTMGEHVGFEFTEVGEDFIKATLPV
                     DHRTQQPYGLLHGGASCVLAETLGSVASAMVVDHDQYMTLGIEINANHMRAMTEGPVT
                     GTCKPLQLGKTLHVWEIRITDTKGRLCCISRLTVMVRKR"
     gene            complement(530..>1450)
                     /locus_tag="PK742_13970"
     CDS             complement(530..>1450)
                     /locus_tag="PK742_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQU77748.1"
                     /translation="DGSTFSDSNTRGFEVNNNQPSAYNVIRNATVNLNNTISLEFFPD
                     VNANISEYTVQRSEDGSSYLNIGNTNFLGGIPLIYYYTDNGVNTRDQFYYYKSAADDA
                     CNLTMESGYARTILLNGSDNADFTNSLNWTPFEMTNAEVLEYRVYRNPGNGDPEVLIA
                     TNTPDHTGLLDNVVESLNAVQQFCYRIEALYHFNAPEVGADYDLLSYSNELCLEQGPR
                     IYVPNAIVPGGLNNEFLPYIIFGKSDGYSMQIFNRYGKLIFESTRTDVGWDGTIDGDP
                     VPMGSYGYLITFTATNGEVITKKGNVTVIR"
BASE COUNT          359 a          348 c          343 g          400 t
ORIGIN      
        1 gacaacaaag aggcgataac agatagtagc tttttcatga tggggtattt tttcaaatat
       61 agaaaaaata atcaagaccc aaaagtgaca ttcctatcgt ttcctcacca tgaccgtcag
      121 ccggctgata caacacagcc tgcctttggt gtctgtgatg cgtatctccc agacatgcag
      181 ggtcttgccc aattgcagcg gtttgcaggt gccggtaacc ggtccttctg tcatggcacg
      241 catatgattg gcgttgatct ctatgccgag ggtcatgtac tgatcgtggt ccaccaccat
      301 ggcgctggcc actgaaccca gggtctctgc cagcacacag gaagcgccgc cgtgcaggag
      361 tccgtagggt tgttgcgtgc gatggtccac aggcagagtg gctttgataa aatcttcacc
      421 cacctcggtg aattcgaaac ctacatgctc tcccatcgtt ccgggattca gtgtcatcag
      481 atcagcgggc tgcagatctt ttttgaacca gatggacatt ggcagaaatt tatctgataa
      541 cggttacatt accctttttg gtgatcacct cgccattggt agctgtaaaa gtgataaggt
      601 atccgtatga tcccatcgga acgggatcac cgtcaatggt tccatcccac cccacatctg
      661 tacgtgtgct ctcaaagatg agcttaccat accgattgaa gatctgcatg ctgtacccgt
      721 cacttttgcc aaatatgatg tatggaagaa attcattatt caatccgccc ggcactatag
      781 cattgggcac atagatacgt gggccttgtt ccagacacag ctcattggaa tagctgagca
      841 ggtcatagtc agcacctact tcaggtgcat tgaaatgata caatgcttct attcggtagc
      901 agaactgctg tactgcattc aggctttcca ccacattatc cagcaagccc gtgtgatcag
      961 gtgtattggt ggcaatgagt acctccggat ctccgttacc cggatttcgg tacacccggt
     1021 attccagcac ttccgcattg gtcatttcga acggtgtcca gttcaggctg ttggtaaaat
     1081 cagcattatc acttccattc agcaggatgg tgcgggcata gcctgattcc atggtcaggt
     1141 tacaggcatc atctgccgca gatttataat aatagaactg atctctggtg tttactccat
     1201 tgtcggtata atagtaaatg agcggaatac ctccaagaaa gttcgtattt ccaatattca
     1261 gatacgagct gccgtcttcg cttcgctgta cagtgtattc gctgatattg gcgttcacat
     1321 caggaaagaa ttccagactg atggtattgt tgaggtttac cgtggcgttg cggataacat
     1381 tataggcaga aggctgattg ttatttactt caaagccacg ggtattactg tcgctgaaag
     1441 tggagccatc
//
LOCUS       DAOMAX010000465         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_25387_length_1445_cov_3.747482, whole genome shotgun sequence.
ACCESSION   DAOMAX010000465 DAOMAX010000000
VERSION     DAOMAX010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25387_length_1445_cov_3.747482"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1286)
                     /locus_tag="PK742_13975"
     CDS             complement(81..1286)
                     /locus_tag="PK742_13975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77749.1"
                     /translation="MKKIWNLYKQVYGGLSAASWMLALIQLINRSGSMVLPFLAVYMT
                     HALGYTVQQAGLIMSIYGVGSLIGSYLGGWLTDKIGAFRVQIFSLVGGGIIFLFLPML
                     KEIEALMAGILISSIVVETLRPANSTAVSQYARPENMTRAFSLNRMAINLGVSVGPAL
                     GGLLAGISYDLLFVADGVTCIAAGLVFYFFFRDRPANVRQKTEEDLANVKKPHKDLVY
                     MIFAVFCGFYALLFLQIFQVLPLYYAGPGALSEAGIGLLLAFNGLFVFVVEMVLVYSI
                     ENRFRLHRIIVTGFLMLSASFLVLLAGPQLPILYTGMLLLSLSEILAMPFMVTLIMKR
                     AGEHARGSYIGIYTMSWSLAFIISPSLTTYIIEHFSYDALWYFNGTLAVVAAVGIGVL
                     AGKMEGRSA"
BASE COUNT          385 a          410 c          336 g          314 t
ORIGIN      
        1 tgactgctga ctgctgactg ctgactgctg actgctgact gctgactgct gactgctgac
       61 tgctgactgc caactgccaa ctacgcactc ctcccctcca tcttccccgc cagcacgcca
      121 atgccgacag ccgcaacgac cgccagcgtt ccattgaaat accaaagggc atcatagctg
      181 aaatgctcta tgatataggt ggtcagtgag ggcgaaatga tgaaggccag tgaccagctc
      241 atggtgtaaa taccaatata gctgcctctt gcatgttcac ccgctctctt catgatgagc
      301 gtaaccatga agggcatggc caggatctct gacaggctta acaacagcat ccctgtgtat
      361 aatattggca gctgcggacc agccagcagc accaggaaag atgccgacaa catcaggaat
      421 ccggtcacga tgatccgatg caatctgaac cggttctcta tgctatatac cagtaccatt
      481 tccaccacga aaacgaacag cccgttaaag gccagcagta aaccgatacc tgcctccgac
      541 aatgccccgg gtcctgcata gtacaaagga agcacctgga atatctgtag aaagagcagg
      601 gcgtagaatc cacagaacac cgcaaagatc atatatacca gatccttatg tggcttcttc
      661 acgttcgcca ggtcctcttc tgttttctgt cgcacattgg caggacgatc ccggaagaaa
      721 aaataaaaga ccagtccggc ggcgatacag gtgacgccat cggctacgaa caacagatca
      781 taagagatac cggccagcaa tcctccgagt gcaggcccga cagaaacacc cagattaatg
      841 gccatcctgt tcaggctgaa ggccctggtc atgttttccg gtctggcgta ctgcgacact
      901 gctgtggaat tggcagggcg caaggtttcc actacaatac tgctgatcag aatacccgcc
      961 atcagcgctt cgatctcttt cagcatggga aggaacagaa agatgatacc ccctcctacc
     1021 agcgaaaaga tctgcacccg gaaagccccg atcttatcgg tcagccatcc tcccagatat
     1081 gacccgatga gcgacccaac accatagata ctcatgatga gacccgcctg ttgtaccgta
     1141 tatcccagcg catgggtcat gtacacagcc agaaaaggca gtaccatact cccgctccgg
     1201 ttgatgagct ggatcagcgc cagcatccag ctggctgccg acagtccgcc gtacacctgt
     1261 ttatataagt tccagatctt tttcaaggcg ggtaaaaata ccggtttggg aagtgatata
     1321 cgttaccatt tcattgttgt ttccatacta tcttgccccc atgaaaaagt ggatgttatt
     1381 gtgcctcccc tggataggtc tggtacatgc acaaacgatg gaggaattct ccgtcagcgt
     1441 cgaag
//
LOCUS       DAOMAX010000466         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_25471_length_1442_cov_3.560202, whole genome shotgun sequence.
ACCESSION   DAOMAX010000466 DAOMAX010000000
VERSION     DAOMAX010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25471_length_1442_cov_3.560202"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PK742_13980"
     CDS             <1..651
                     /locus_tag="PK742_13980"
                     /inference="COORDINATES: protein motif:HMM:NF015657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77750.1"
                     /translation="GVSPAIYPTGIGGYQINTTSAFFINDLHFGPSPVDTFEAGGYFN
                     IFFMDTPPERPTYELLMGSLGETPVEPRLVIPANEIATFTTRYKVQDDMSLLTVNPHM
                     HLLGKTFLAYALTPQQDTIRLIRIPEWDFRWQYFYTFQNPVHIPAGSEIIVEAGFDNT
                     SDNPNNPYDPPRVIVERAHPWESMRTTNEMLQFIITYMPYRPGDEAIDLDHTSLNR"
     gene            648..>1442
                     /locus_tag="PK742_13985"
     CDS             648..>1442
                     /locus_tag="PK742_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQU77751.1"
                     /translation="MSKRWSYITRPILLLSGVSLFTDMASEMLYPIMPVYLESIGFSV
                     LLIGILEGLAETTAGLSKGYFGQLSDLRGSRLPFIRLGYTLSALSKPLLAVSVWPLWV
                     FGARTVDRLGKGVRTGARDAMLSDQSDPEYRARVFGFHRGMDTLGAVIGPLLALWYLQ
                     KHPDDYQTLFLLAFGPGAMSILLLYLLREKKKEVQQVRPGLFDFLKYWKDSPSAYRHL
                     LAGLLLFALFNSSDVLLLLRIKESGWSDAAVIGVYIFYNLVYAIGAF"
BASE COUNT          335 a          380 c          354 g          373 t
ORIGIN      
        1 ggcgtttcac ccgccatcta cccaacagga ataggcggat atcagatcaa taccaccagc
       61 gccttcttta tcaatgacct gcattttggc ccgtcgccgg tagatacctt cgaagcagga
      121 ggctacttca atatcttttt catggacacc cctcctgaac gccccaccta tgaactgctc
      181 atgggttctt tgggagaaac accggtagaa ccgcggctgg tcattccggc taatgagatc
      241 gccactttca ccaccaggta taaggttcag gatgatatgt cactgctaac ggtcaatccg
      301 catatgcacc tgctgggcaa aactttcctt gcctatgcgt tgacaccaca acaggatacc
      361 atcaggctga tcaggatccc ggaatgggat ttccgctggc agtattttta tacttttcag
      421 aatccggtgc atataccagc aggttctgag atcattgtgg aagctgggtt tgacaacaca
      481 tcagacaacc caaacaaccc ttatgaccct ccacgtgtca tagtagaaag agcgcatccc
      541 tgggaaagta tgcgcactac caatgaaatg ttgcaattca tcatcactta tatgccttat
      601 cgccccggcg atgaagcaat agatctggat cataccagcc tcaacagatg agtaagagat
      661 ggtcttatat cacccgcccc atcctcctgc tgagcggtgt cagtctgttt acagatatgg
      721 ccagtgagat gctgtatcct attatgccgg tgtatctgga aagcatagga ttctcggtcc
      781 tgctcattgg aattctggaa ggacttgcag agaccacagc cggattgagt aaagggtact
      841 ttgggcaact ctctgacctg aggggcagcc gcttgccatt tattcgcctc ggatataccc
      901 tgagtgcgct gagcaaaccc ctgctggctg taagcgtgtg gccactttgg gtgtttggtg
      961 caagaaccgt tgaccgtctt ggaaaaggcg ttcgtaccgg cgccagagat gccatgctct
     1021 ctgatcagtc tgacccggaa taccgggcca gggtattcgg tttccatcgt ggtatggata
     1081 ccctcggagc agtgatcggg ccgttgctgg ccctttggta cctgcagaaa catcctgatg
     1141 attaccagac ccttttcctg ctggcatttg gccccggggc catgagcatc ctgttgctct
     1201 acctcttgcg tgaaaagaaa aaagaagtgc agcaggtgcg ccccgggctc tttgattttc
     1261 tgaagtactg gaaagacagt ccgtcagcat accggcacct gcttgccggt ctcctgttgt
     1321 ttgcactctt caatagttct gatgtattgc tgttactgcg catcaaagaa agcggctgga
     1381 gtgacgcggc agtcattggt gtctatatct tttacaacct cgtatatgcc attggagcct
     1441 tc
//
LOCUS       DAOMAX010000467         1437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_25631_length_1437_cov_3.675832, whole genome shotgun sequence.
ACCESSION   DAOMAX010000467 DAOMAX010000000
VERSION     DAOMAX010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25631_length_1437_cov_3.675832"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1437
                     /locus_tag="PK742_13990"
     CDS             <1..>1437
                     /locus_tag="PK742_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77752.1"
                     /translation="IRIINGAGSEKLSCNFFLILKKNEMRPILLFLLFSLAIGTAKAL
                     IIITFPSAIPDKCFVALYDDFYNPIASGLINCGGLLVPDGKYDINKESDFIRDLQKKN
                     PKLSFEEATVRSVSEAEFLSDSRYQKDQCVHSGDLPAKSLSEIIVVTPEPAGILVAVG
                     CHYGVTTAVTFDDSGYHFHEFHSIPCSGNWAVSFYSPPPGDGTGTEQAGNELYKEEEL
                     QAGISIFSSDVIPEEVYSSRDPDEKYKEFLSLIDYSLPPAVIAAEKLSDPWYKRIITM
                     KENPGLATGGSQGTVLCSYECDYEGYRIEVIEEPGGARRIRSWTKNIEYYAGLDEIIQ
                     DFGNVTPDYNTCGGCSVTQVWNGQGVSPFCNRSGQPISGPAYHVSILNDNSYDGSTLP
                     LAGFDCSDYDRAALVNVFNLSPNPGFDLPTTCGVACVNVGFQPGGDPSDTIGLGHSWD
                     EWNSDAYHETLWDIWGSFGGAPVQPSSE"
BASE COUNT          389 a          270 c          348 g          430 t
ORIGIN      
        1 ttatcagaat cattaatggg gcaggttcag aaaagttatc atgcaatttt tttttaattt
       61 taaaaaaaaa tgaaatgcga cctattctgc tctttttact ttttagtctt gcaatcggta
      121 cagcaaaggc cttaattatt attacatttc cttctgcaat acctgacaag tgttttgtgg
      181 ctttgtatga tgatttttat aacccaattg catcgggttt gatcaactgc ggtggactgt
      241 tggttcctga tgggaaatat gatattaaca aagaatcaga cttcatacgt gatctgcaga
      301 agaagaatcc caagttgtct tttgaagaag ctactgtaag atccgtatca gaagctgagt
      361 tcttgtcaga ttctagatac cagaaagacc aatgtgttca cagtggtgac ctcccggcga
      421 aatcattatc ggagatcata gtcgtcactc cggaacctgc tggaatactg gtcgcagttg
      481 gttgtcatta cggtgtgacc acagcagtta cgtttgatga tagcggatat cattttcacg
      541 aatttcattc tatcccatgt agcgggaatt gggctgtttc attctattct ccacctccgg
      601 gtgatggaac aggtacagaa caggcgggaa atgagttgta taaagaagaa gaattacagg
      661 caggaattag tatattctca tctgatgtaa taccggaaga ggtttatagt agtcgtgatc
      721 ctgatgaaaa gtacaaagag ttcctttcct tgattgacta ttcactccca cctgcagtta
      781 ttgctgctga gaagctttct gatccttggt ataagagaat cattacgatg aaagagaacc
      841 ccggccttgc tacaggtggc tcacagggta ctgttttgtg tagttatgaa tgtgactatg
      901 aagggtatcg tattgaagtg atcgaagagc cgggtggtgc gagacgtatt cgttcgtgga
      961 ccaaaaacat tgagtactat gcaggtctgg atgagatcat tcaggatttt ggaaatgtta
     1021 cacctgacta taatacctgc ggaggatgct cagttacaca agtgtggaat gggcaaggag
     1081 tctcgccatt ttgtaacaga tcaggacaac caatttcggg accagcttat catgtttcca
     1141 ttttgaatga caacagctat gatggcagca ccttaccgct ggcaggcttt gattgcagcg
     1201 actatgatcg agcagcattg gttaacgtat ttaatctttc gccaaacccg ggttttgatc
     1261 tgcccacaac ctgtggggta gcttgtgtca atgtgggttt tcagccaggg ggcgatccgt
     1321 ctgatacaat aggtttgggt cattcatggg atgagtggaa ctctgacgca tatcatgaaa
     1381 cgctttggga tatttggggg tcatttggcg gggcccctgt ccaaccaagc agcgaaa
//
LOCUS       DAOMAX010000468         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_25708_length_1435_cov_3.454348, whole genome shotgun sequence.
ACCESSION   DAOMAX010000468 DAOMAX010000000
VERSION     DAOMAX010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25708_length_1435_cov_3.454348"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..616)
                     /locus_tag="PK742_13995"
     CDS             complement(212..616)
                     /locus_tag="PK742_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQU77753.1"
                     /translation="MKFLNPKFAWTNFGLLFLRITLGASFIYHHGYGKISDPSRWKGL
                     GEQMSNVGIHFLPEFWGFMAAFAEFFGSVLLILGLFTRPATILLAFTMLMAVIKHSQA
                     GEGFAYPLDMLLVFLAIFFTGPGKYSLDARLR"
     gene            complement(840..>1435)
                     /locus_tag="PK742_14000"
     CDS             complement(840..>1435)
                     /locus_tag="PK742_14000"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HQU77754.1"
                     /translation="NICVYTDPGLLFGAPEMIGEAGKTYNLTIEAEGKTLTSTTTIPP
                     IVELDSLFYLPHEDPGAADSLVRLYAHLTDPPELGNYYRLFTSRNNGAYQTDFFSVTD
                     DLVFNGLSIDFTINRILEDGEEFNGDTFGYFFRGDTVAVKLCSIDQATFDFWNTLEND
                     SGGDGPFSSITIVRTNINGGQGIWCGYGAGISTIVIE"
BASE COUNT          362 a          381 c          345 g          347 t
ORIGIN      
        1 tatgatcacc cctccccctt cgggtactcc ctcccgattt tcatcgggac aagctaggag
       61 acaacttcct ttgtttcctc agctataaga gacccctgct acgccttcac tacaatggga
      121 acaaagacag aattgcagaa ggtcggcggg tactccctcc tgattttcat cgggacaagc
      181 ttggagacta tttcctttga taccacaccc atcacctcaa cctggcatcc aggctgtatt
      241 tgccgggacc ggtgaagaag atggcaagga acaccagcag catatcgagc ggataggcaa
      301 agccctctcc agcctgcgaa tgcttgatga ctgccatcag catagtgaat gcaagaagta
      361 tggtagcagg gcgggtgaac aatcccagta tcagcaggac actgccaaaa aactcggcga
      421 atgccgccat gaaaccccag aactcaggca aaaagtggat gccaacattg gacatctgct
      481 caccgagtcc tttccacctg gaaggatccg atattttgcc ataaccatgg tggtaaatga
      541 aggaagcccc cagcgtgatg cggaggaaca gcaaaccgaa gtttgtccag gcgaatttgg
      601 gatttaggaa tttcaaagtc gtggtgcgtt attcgttatt cgtggtgcgt tgatcgttgt
      661 tcgttatgcg tggttcgttg atcgttgttc gtataattcc tgattggaag accctcaccc
      721 tctcacttcc cgccagggcg ggattcactc ctcacctcat atgacaactc tcaaattacc
      781 agataagttc ttggttgttg gtcattagta attggttgag acccgatact cgctacctgc
      841 tactcaatga ctatcgtcga tatcccagcc ccgtaaccgc accagatgcc ctggccgccg
      901 ttgatattgg tcctgacaat ggtgatggag ctgaaaggac cgtcgccgcc actgtcgttc
      961 tccagggtat tccagaaatc gaacgtggcc tgatcaatag aacacagttt tacagcaacc
     1021 gtatcgccgc gaaagaaata accgaacgta tctccgttga attcttcccc atcttccagg
     1081 atgcggttga tggtaaaatc tatcgacagc ccgttgaaca cgagatcgtc ggtcacagag
     1141 aagaaatcgg tctgataggc accgttattc ctggacgtga acaaccgata gtagtttccc
     1201 agttcgggcg gatcggtcag gtgggcatac aaacgcacca gcgaatctgc cgcacccgga
     1261 tcctcatgtg gcaaatagaa caaactgtcc agctccacga tcggcgggat ggtagtagtg
     1321 cttgtaaggg tcttcccttc tgcttctatc gtcaggttgt aggtcttgcc tgcttcgccg
     1381 atcatctccg gtgcaccgaa caataatccc ggatcggtat agacacaaat attat
//
LOCUS       DAOMAX010000469         1418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_26314_length_1418_cov_3.903155, whole genome shotgun sequence.
ACCESSION   DAOMAX010000469 DAOMAX010000000
VERSION     DAOMAX010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26314_length_1418_cov_3.903155"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1147)
                     /gene="hppD"
                     /locus_tag="PK742_14005"
     CDS             complement(17..1147)
                     /gene="hppD"
                     /locus_tag="PK742_14005"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HQU77755.1"
                     /translation="MSVETLTKNSTQTTEDFLPLLGTDHIEFYCGNAKQSAYYYQSAW
                     GYELIAYAGLETGVKDRTSYVLRQGKITLVLTTSFDPDSEISQHVRKHGDGVKYLSLW
                     VDDARKSFEETVKRGAKPAMEPTVFKDEHGEVVISAIHTYGDTIHKFVERKNYNGAFL
                     PGFVEKHSNMEIKPIGLMYVDHCVGNVELGGMNKWVEFYENVMGFKLLITFDDKDIST
                     EYSALMSKVVSNGNGYIKFPINEPAEGKKKSQIEEYLEFYKSAGVQHIAVATDDIIFT
                     VGELRKRGVEFLYVPDTYYEDVMDRVGAIEEDLEVLKSLNILIDRDEEGYLLQLFTKP
                     VEDRPTVFYEIIERNGAKSFGKGNFKALFESIEREQELRGTL"
     gene            complement(1230..>1418)
                     /locus_tag="PK742_14010"
     CDS             complement(1230..>1418)
                     /locus_tag="PK742_14010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQU77756.1"
                     /translation="SRADFIHKIKIAAKETREVMYWATLCKHAESYPEPVAIIEIAIN
                     LNRILSKIIISSRSSKRQ"
BASE COUNT          322 a          353 c          300 g          443 t
ORIGIN      
        1 tttatgtctg aaagtatcag agcgtccctc gcaattcctg ttctctttct atcgattcga
       61 acagggcctt gaagtttcct ttcccaaaac tcttggcgcc gttgcgctca atgatctcat
      121 agaatacagt gggcctgtct tccaccggct tggtgaataa ctgcagcagg tatccttctt
      181 cgtcgcggtc tatcaggatg ttcaggctct ttagcacttc caggtcctct tcaatagcac
      241 cgacccgatc catgacgtct tcgtaataag tgtcaggtac atagaggaat tccacgccac
      301 gcttgcgcaa ttctcctact gtgaagataa tatcgtcagt tgctaccgcg atatgctgca
      361 cacctgcact cttgtagaat tccagatact cttctatctg agatttcttc ttgccttcag
      421 ccggctcatt gatcgggaat ttgatgtagc cgttgccatt gctcactacc ttactcatca
      481 gcgcagagta ttcagtggag atgtccttgt catcaaaagt gatcagcagc ttgaagccca
      541 tcacattctc atagaattcc acccatttgt tcattccacc cagctccacg ttacctacgc
      601 agtggtccac atacatcaga ccgataggct tgatctccat attgctgtgc ttctctacga
      661 atcctggcag gaaagctccg ttgtagttct tgcgttccac gaatttgtgg atggtatcgc
      721 cataggtatg gatggctgat atgaccacct caccatgttc gtccttgaat actgttggct
      781 ccatggctgg tttggcacca cgcttcaccg tttcttcgaa tgactttcgg gcatcgtcca
      841 cccatagtga cagatatttg acaccatcgc catgcttgcg cacatgctgt gagatctcgc
      901 tgtccggatc gaaactggtg gtcagcacca aagtgatctt accctggcgg agcacataag
      961 aagtgcggtc ctttacaccg gtctccagtc ccgcataggc gatcagttca tatccccatg
     1021 ccgactgata atagtacgcc gactgtttag cgtttccgca atagaattca atgtggtcag
     1081 ttcccagtaa cggcagaaag tcttctgtgg tctgggtgga gtttttggtc aatgtttcta
     1141 cgctcatgtt agtttgattt ataagccaat tagccaatta gctttattca tagtaagtaa
     1201 tgtgaaaatt tgaaaatgtg gaaatgtgtt cattgccgtt tacttgatct tgagctaatt
     1261 atgatcttgc ttagaattcg gttcagattt attgctattt caatgatagc caccggctca
     1321 gggtatgatt ctgcatgttt gcaaagagtt gcccaataca tcacctctct ggtttctttg
     1381 gctgctattt tgattttatg aataaaatct gccctgct
//
LOCUS       DAOMAX010000470         1415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_26401_length_1415_cov_4.975735, whole genome shotgun sequence.
ACCESSION   DAOMAX010000470 DAOMAX010000000
VERSION     DAOMAX010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26401_length_1415_cov_4.975735"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK742_14015"
     CDS             <1..430
                     /locus_tag="PK742_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77757.1"
                     /translation="SSGIFVYFDSKEWLTGLGDYTYGPFSAYREIVFGGGVEYNGYIS
                     GGEVLWGDPARCLATDLIDDNQSNKLTISPNPNNGTFLLQSDTWMDAIRLYSGRGQQV
                     YQLHDAGLQTTIDLPLDPGMYLLHIIRNDQLVQKKFIVQL"
     gene            488..>1415
                     /locus_tag="PK742_14020"
     CDS             488..>1415
                     /locus_tag="PK742_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQU77758.1"
                     /translation="MTEHNPTFDLAAQLALYTYRHLFLTGKAGTGKTTFLRHIQDETS
                     KKTAVVAPTGVAAINAGGVTMHSFFQLPFEPFIPVRGFMPDSVQVANRNNLFKKIRLS
                     AVKREVLEELEMLIIDEVSMLRSDQLDCMDAILRGIRRNDQPFGGVQVIFIGDLYQLP
                     PVVTDNDKHILDEYYDSPFFFDAKVLQQTELLHIELTKVYRQQEQTFVDLLNKVRHND
                     IGPDEEALLQERFAPQFRPDASDGFITITSHNYKADTINMRELAQLPGEMHTFKGEIT
                     GEFGDKSLPTELDLQLKPGAQVMFVRNDVGEHR"
BASE COUNT          370 a          397 c          354 g          294 t
ORIGIN      
        1 gtcatcagga attttcgtct atttcgattc aaaggaatgg ctaacaggcc tgggtgatta
       61 cacttatgga ccattcagcg catatagaga gatcgtattc ggtggaggag ttgaatataa
      121 cggctacatc agtggcggag aagtgctttg gggtgatcca gcaaggtgtc tggcaacaga
      181 tttaatagat gacaatcaaa gcaacaagtt aaccatcagc cccaacccga acaacggcac
      241 cttcctgctg cagagcgaca cctggatgga tgcgatcagg ctctactccg gtcggggaca
      301 acaggtatat cagctacatg atgcaggcct tcaaaccacc atagacctgc ctcttgatcc
      361 gggtatgtac ctgctgcaca ttatccgtaa tgaccagttg gtgcaaaaga aatttattgt
      421 gcaactttaa cgcactacac cacacatcct gcccgaggca atttattaac ttgcccaccc
      481 cttcaggatg accgaacaca acccgacatt cgaccttgca gcgcagctgg cactgtacac
      541 ctacagacac ttgtttctga ccggtaaggc cggtaccgga aagaccactt tcctccggca
      601 tatccaggac gaaaccagca agaaaacggc cgtggtagca cccaccggtg tggcggccat
      661 caatgccggc ggggtcacca tgcactcctt ctttcaactt ccctttgaac ccttcatacc
      721 ggtcaggggc ttcatgcccg attccgtgca ggtggccaac cgcaacaacc tgttcaagaa
      781 gatcaggctg agtgccgtga agcgggaagt gctggaagaa ctggagatgc tcatcatcga
      841 tgaagtgagc atgctgcgca gcgatcagct ggattgcatg gatgccatcc ttcggggtat
      901 ccgcaggaac gaccagccct ttggaggtgt tcaggtcatc ttcatcggcg atctgtatca
      961 gttaccgccg gtggtgacag acaatgacaa gcatatcctg gacgaatatt atgacagtcc
     1021 tttcttcttc gacgccaaag tgctgcaaca gacagaactg ctgcacatcg aactgacgaa
     1081 ggtctatcgc cagcaggaac agaccttcgt ggacctgctc aataaggttc gccacaacga
     1141 catcggtccg gatgaagaag ccttgctgca ggaacgcttc gccccgcaat tccgccccga
     1201 cgcatctgat ggtttcatca ccatcacatc ccacaactac aaggcagaca ccatcaacat
     1261 gcgggagctg gcacagctgc ctggcgagat gcacacgttc aaaggagaga tcacgggaga
     1321 gttcggcgac aagagcctgc ccaccgaact ggacctccaa ctcaaacccg gcgcacaggt
     1381 catgttcgtg cgcaatgatg tgggtgaaca ccgcc
//
LOCUS       DAOMAX010000471         1411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_26584_length_1411_cov_4.662242, whole genome shotgun sequence.
ACCESSION   DAOMAX010000471 DAOMAX010000000
VERSION     DAOMAX010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26584_length_1411_cov_4.662242"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..661)
                     /locus_tag="PK742_14025"
     CDS             complement(128..661)
                     /locus_tag="PK742_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQU77759.1"
                     /translation="MQKAVWALDVTHSEITFKVKHMMVTNVTGKFEDYSVTADIDEEN
                     FENSQVSFTAKTSSVNTGVADRDNHLRSADFFDSENHPEMTFTSTSFEKVDDESYRMT
                     GNLNIRGIEKPVTLNVEVGGFGKDPWGNTKGGFTISGKINRKEWGLNWNAALEAGGVL
                     VAEDVRINCEVQLMKQA"
     gene            complement(697..1410)
                     /locus_tag="PK742_14030"
     CDS             complement(697..1410)
                     /locus_tag="PK742_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQU77760.1"
                     /translation="MLHPAREKGHANHGWLDSYHSFSFAGYYDPDKVHFGALRVLNDD
                     TVAAGMGFGTHPHDNMEIISIPLSGDLEHRDSMGNTTVIRHGDIQVMSAGTGVQHSEY
                     NKNADKEVKFLQIWVFPNKRNVAPRYDQITLDPAARHNKLQQILSPNANDEGVWIHQD
                     AWFHLGHFDKDQAFGHTLNKPGNGIYAFVLEGSFRVHGKELGRRDAAGISDVTNIELE
                     SLEDGSEILLMEVPMTFTY"
BASE COUNT          304 a          363 c          338 g          406 t
ORIGIN      
        1 cggagagaca tgtggggatc gagtgtttcc attttagata cccacattac cagataggaa
       61 ctctgagagg atataagaaa aatgggagac ccacaggcct cccattttat gattggttat
      121 tatagaatta tgcctgcttc atcagttgca cttcacagtt gatgcgcaca tcttctgcta
      181 ccagtacgcc accggcttcg agggctgcat tccagttgag tccccactcc ttgcggttga
      241 tctttccgct gatggtgaaa ccacccttgg tgttgcccca cgggtcttta ccaaaaccac
      301 ctacctctac attcagggtc accggcttct cgatcccgcg gatgttcagg ttaccggtca
      361 tccggtagct ctcgtcatct actttttcga aagaggtact ggtaaaggtc atttccgggt
      421 gattctcact gtcgaagaag tctgcactgc gcaggtggtt gtcgcggtct gccacgccgg
      481 tgttgacact tgaggtcttc gcagtaaagc ttacctgtga gttttcgaag ttttcttcat
      541 cgatgtcggc cgtaacactg tagtcttcaa acttaccggt tacgttggtc accatcatgt
      601 gttttacctt gaaggtgatc tcagaatggg tcacatcaag tgcccatact gctttctgca
      661 ttgttgattg ttcgctcata ttttattgat ttggttttaa tatgtaaagg tcatgggtac
      721 ttccatcagc aggatctcag agccgtcttc cagcgattcc agttcgatgt tggtcacgtc
      781 gctgatacct gcggcatctc tgcgtccgag ttcctttcca tgaacacgga agctaccttc
      841 cagcacaaaa gcatagattc cgttgcccgg cttattcaat gtatggccaa aggcctgatc
      901 cttgtcgaaa tgtccaagat ggaaccaggc gtcctggtgg atccagacac cttcatcgtt
      961 tgcgttgggc gacaggatct gttgcagctt gttgtgtctt gctgccggat ccagggtgat
     1021 ctggtcatac cgaggtgcta cattcctttt attaggaaac acccagatct gcaggaactt
     1081 cacttccttg tctgcattct tattgtattc gctgtgctgc accccggtgc cggcactcat
     1141 gacctgaata tcgccatggc gtatcaccgt ggtattaccc atgctgtcgc ggtgttccag
     1201 atcgcccgac aaggggatgc tgatgatctc catattatca tgcgggtgcg taccgaatcc
     1261 catgccggca gctacggtat catcattcag tacccgcagg gcaccaaagt gtaccttgtc
     1321 aggatcatag taacctgcaa aactgaaact atgataagaa tccagccagc cgtggttggc
     1381 atgtcctttc tctctggcgg gatgcagaat a
//
LOCUS       DAOMAX010000472         1407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_26736_length_1407_cov_4.169379, whole genome shotgun sequence.
ACCESSION   DAOMAX010000472 DAOMAX010000000
VERSION     DAOMAX010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26736_length_1407_cov_4.169379"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /gene="galE"
                     /locus_tag="PK742_14035"
     CDS             complement(<1..1012)
                     /gene="galE"
                     /locus_tag="PK742_14035"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQU77761.1"
                     /translation="MKTCKVLVTGGCGYIGSHTAIDLLKQGFEVVSIDSLERSQAFVA
                     GRVEEISGKPFHQYSFDCRDEAAVRKVFEQHPDISGVIHFAAYKSVGESVEHPLRYFD
                     NNLNSLITVLRVAESFHVQHFVFSSSCSVYGNAASLPVTESTPLAEPESPYGRTKLMS
                     EQIIHDYSLHSRMRFTVLRYFNPAGAHDSGRLGEIPFGKPDNLVPVITETAIGKRPAL
                     TVYGDDYPTRDGSCLRDYIHVMDIASAHTKALQYLLENKQKEKVEIFNLGSGEGITVL
                     EMIHAFEKVSATELNYLLGDKRPGDVIAIYADNSKARSLLGWIPVRTTEDIMRSAWAW
                     ENR"
     gene            complement(1009..>1407)
                     /locus_tag="PK742_14040"
     CDS             complement(1009..>1407)
                     /locus_tag="PK742_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQU77762.1"
                     /translation="IEEKPAAPKSNFAVPGLYFYDNEVVDIARNLKPSARGEYEITDV
                     NKEYLRRDKLRVTVLPRGTAWFDTGTFDSLMQASQFIQVIEQRQGLNIGCIEEIAYRK
                     KFIDAAQLEAIAQPLMKSGYGQYLMRQLKR"
BASE COUNT          316 a          355 c          359 g          377 t
ORIGIN      
        1 tccggttctc ccaggcccag gcagaacgca tgatgtcttc tgtggtgcga acggggatcc
       61 atcccaataa tgatcttgcc ttgctgttat ctgcgtagat ggcgatcaca tcgccgggtc
      121 ttttgtcacc cagaagatag ttgagttcag tagcacttac tttttcaaag gcgtggatca
      181 tctccagtac ggtgatgcct tctccgctgc ccaggttgaa gatctccacc ttttctttct
      241 gcttgttttc cagcaggtat tgcaatgctt tggtatgggc tgaagcaatg tccatcacat
      301 ggatgtagtc acgcagacag gagccatcgc gggtagggta atcatcgccg taaacggtca
      361 gcgccggtct tttgccgatg gcagtttcgg tgatgaccgg aaccagattg tcgggcttcc
      421 cgaaggggat ctcacccagc ctgccactgt catgggcacc cgccggattg aaatagcgca
      481 gtacggtgaa acgcatcctg ctgtgcagac tgtagtcatg aatgatctgt tcactcatga
      541 gcttggttct gccatagggc gattcaggtt ctgccagcgg tgtggattca gttaccggaa
      601 gggaagccgc attaccatag accgagcagc ttgaggaaaa tacaaagtgc tgtacatgga
      661 aagactctgc caccctcaac acggtgatca gcgagttcag gttattgtcg aaataacgca
      721 acgggtgttc tactgattcg ccgacagact tataggctgc aaaatgaatg acaccgctga
      781 tatcagggtg ctgttcaaaa actttccgca ctgcagcttc atcgcggcaa tcgaaagagt
      841 attgatgaaa cggttttccg ctgatctctt ctacccgtcc ggccacaaag gcctgtgaac
      901 gttccagact gtctatggac actacttcaa agccctgttt cagcagatcg atggcagtgt
      961 ggctgcctat atatccgcat cctccggtta ccagtacctt acaggttttc atcgtttcag
     1021 ttgacgcata aggtattgac cgtaaccact cttcataagt ggctgggcga tggcttccag
     1081 ttgggctgca tcaatgaatt tctttctgta ggcgatctct tctatacagc cgatgttcag
     1141 accctgtcgc tgttcgatca cctggatgaa ctggctggcc tgcatcaggc tgtcgaaggt
     1201 acctgtatca aaccaggctg tacctcttgg tagcaccgta acacgcagtt tatctctgcg
     1261 aaggtattcc ttgttcacat cggtgatctc atattcaccg cgggctgaag gcttcaggtt
     1321 gcgggcaatg tccactactt cattgtcgta gaaatagagg ccgggaaccg caaagttgga
     1381 tttaggagct gcaggttttt cctcaat
//
LOCUS       DAOMAX010000473         1394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_27212_length_1394_cov_4.285288, whole genome shotgun sequence.
ACCESSION   DAOMAX010000473 DAOMAX010000000
VERSION     DAOMAX010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27212_length_1394_cov_4.285288"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..864
                     /locus_tag="PK742_14045"
     CDS             109..864
                     /locus_tag="PK742_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQU77763.1"
                     /translation="MGREYRWENTPKCIVSLVPSITELLFDLGLQEQVVGVTKFCVHP
                     ENSRKEKTIVGGTKNLRMDVISKLQPDLIIANKEENNRDDILQLAEEFPVFISDVSTV
                     EDALEMIMSVGRITDRGEQSLLICHRIIEARNEWYRHRPKGIRTLYLIWREPWMCAGA
                     DTFIHHMMEEAGLENVVEANRYPKTNIEEMQAIHPQLVMLSSEPYPFSDEHLHELQQE
                     LPDSRIILVDGEMFSWYGSRMQYSYPYFSKLMY"
     gene            complement(861..>1394)
                     /locus_tag="PK742_14050"
     CDS             complement(861..>1394)
                     /locus_tag="PK742_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HQU77764.1"
                     /translation="FAILREGFETALFLVASTSIDQSFSYTGFFGGLLLAVLIGYLIV
                     VQGRRIQLKKFFSYTSLLLVVFAAGMIAHGTHELHEYLEYKEHASEGMVISEEEEGEE
                     EEAVYSIFEPKTTDPGSFWYKQQGGQYVHILNDKGSVGSFFKGLFGYNSDPIWAEFIL
                     WWVSLIFGLVLWRRMYR"
BASE COUNT          410 a          338 c          323 g          323 t
ORIGIN      
        1 acatgaaaat cctgcagtac tggtgaaaga ttttaaggtt gacaagacct atattattac
       61 ccggtacgac atcatcaatg cactgacctg atgcacattt ctgatcagtt gggtagagaa
      121 tataggtggg agaatacacc caaatgcatc gtgtcactgg tgccttccat cactgagctg
      181 ctttttgatc tcggactgca ggaacaggtg gttggtgtga ccaaattctg tgtgcatcct
      241 gaaaacagca gaaaggaaaa gaccatagta ggcggaacaa agaacctgcg gatggatgtg
      301 atcagcaaac tgcaacctga tctaatcatc gctaataaag aagaaaacaa ccgggatgat
      361 attcttcaac tggccgaaga atttcctgtt ttcatcagtg atgtatcgac tgtggaagat
      421 gccctggaaa tgatcatgtc ggtagggcgt atcactgatc gcggcgaaca gtctttactg
      481 atctgtcacc gcatcatcga agccagaaat gaatggtacc gccacagacc aaaaggtatc
      541 cgaacactct atctcatctg gcgtgaaccc tggatgtgcg ccggtgcaga tactttcatc
      601 caccatatga tggaagaagc aggactggaa aatgtggtgg aagccaaccg ttatcccaaa
      661 accaacatag aagagatgca ggcaatacat ccgcagctgg tgatgctctc ttcagagcca
      721 taccctttca gcgatgaaca cctgcatgaa ctgcaacagg aattgccaga cagcaggatc
      781 atattggttg acggagagat gttcagttgg tacggcagcc ggatgcaata cagttatccg
      841 tatttcagta aactgatgta ttagcgatac atcctcctcc ataacaccag gccgaagatg
      901 agtgacaccc accagagaat gaactctgcc cagatgggat cgctgttata accgaacaga
      961 cctttgaaga aagaacccac actgcccttg tcattcagga tgtggacgta ctgaccgccc
     1021 tgttgcttgt accagaaact tccaggatcg gtggtcttcg gctcgaagat gctgtacaca
     1081 gcttcttctt cttcgccttc ttcctcttca ctgatgacca taccttctga tgcatgctct
     1141 ttatactcca ggtactcatg cagttcatgc gtgccatgag cgatcatacc ggcagcaaaa
     1201 acaaccagca acaaggatgt ataactgaag aatttcttca gctgtatgcg tcggccctgt
     1261 accacgatca gataaccgat cagcaccgca agaagtagtc cgccgaaaaa accggtatag
     1321 gaaaagctct ggtcaatact ggtggacgcc accagaaaaa gggcggtttc aaaaccctcc
     1381 cgcagaatgg caaa
//
LOCUS       DAOMAX010000474         1389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_27409_length_1389_cov_3.018741, whole genome shotgun sequence.
ACCESSION   DAOMAX010000474 DAOMAX010000000
VERSION     DAOMAX010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27409_length_1389_cov_3.018741"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..690
                     /locus_tag="PK742_14055"
     CDS             166..690
                     /locus_tag="PK742_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU77765.1"
                     /translation="MRRKLQPFLQHMPAGSSVLDIGSGGGLATSVLREAGFQVTPMDI
                     QDGAYDDSTRPVVYDGKTFPFSDRQFDFGLLLTVLHHIKDPDAVLSEAFRTCRTVLIV
                     EDIYRNPAQQYLTYATDWFVNLFYSPNPHTNRNDAQWKETFQRFDKQLIAVTYKPLLG
                     LYLQAWYVVGTQEV"
     gene            complement(678..>1389)
                     /locus_tag="PK742_14060"
     CDS             complement(678..>1389)
                     /locus_tag="PK742_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQU77766.1"
                     /translation="GYSGKVILDNVNLDLEQGTFHVILGLNGAGKSTLLRTLAGLQPG
                     ISGQVIAGERPLASISLKEQAALRSIVLSGRQDVVGGLLVEEVLQMAMSVNNNTEENK
                     NRIVGLLHIEAFRNRRLHTLSDGEFQRVMIARALLQDTPFVFMDEPTAHLDVVARIEL
                     MQLLQHLPEELGVGLIITTHEVELVAQAAGHFIIIDADRNVHAGDADWLKREHMISRT
                     FGGDKVRYDEAQGRFYTS"
BASE COUNT          307 a          401 c          328 g          353 t
ORIGIN      
        1 gccttcggtc agcctaccgg tctgcgttac tgcatcaact ccgtctccct gcatttcgag
       61 ccggagaaat aagtttccgc ctaccttcgc agggtgatcc tgtacacgat acaccggatt
      121 ccttttttgc gcaactggta tttccacgca gccggaagat ccttcatgcg cagaaaactg
      181 cagccgtttt tgcagcacat gccggcaggc agttctgtgc tggatatcgg aagtggtggt
      241 ggattagcta cttccgtact tcgggaggcc ggatttcagg tcactccgat ggatattcag
      301 gatggtgcgt atgacgatag cacccgcccg gtcgtgtatg atggcaaaac cttcccgttc
      361 tcagatcgac aatttgattt tggattattg ctgacagtgc tgcaccatat caaggacccc
      421 gatgcggtgc tgtctgaagc cttccgtacc tgccgtactg tgctgatcgt ggaggacatt
      481 taccgtaatc cggcgcagca atatcttacc tatgccaccg actggttcgt gaacctgttc
      541 tactccccca atccgcatac gaacaggaat gatgcacaat ggaaggagac ctttcaacga
      601 ttcgacaaac aattgatagc ggttacctac aagcctttgc ttggacttta tctgcaggcc
      661 tggtatgtgg taggaactca ggaagtatag aacctgccct gcgcttcatc gtacctgacc
      721 ttatcgcctc cgaaagtccg gctgatcata tgctcccgct tcaaccaatc cgcatctccg
      781 gcatgcacat tcctgtcggc atcaatgatg atgaaatgtc cggctgcctg agccaccagt
      841 tccacttcat gggtggtaat gatcagcccc acccctaatt cttccggtaa atgctgtaac
      901 aattgcatca gctctatcct ggccaccacg tccagatggg cagtcggttc atccatgaac
      961 acaaaaggtg tatcctgcag caatgctctg gcgatcatga cccgctgaaa ctcgccgtcg
     1021 ctgagtgtgt gcaatcgccg gttcctgaaa gcttcaatat gcagcagtcc aacgatcctg
     1081 tttttatttt cctccgtatt gttgttcacc gacatggcca tctgcaacac ctcctcaacc
     1141 aacaatccgc ctaccacatc ctgtcgcccg ctcagcacga tgctgcgcag cgctgcctgt
     1201 tctttcagag agatggatgc gagtggtctt tctccagcga tgacctgacc ggaaatgcct
     1261 ggctgaagac ctgccagagt gcgcagcaga gtggacttcc ctgcaccgtt cagtcccagg
     1321 atgacatgga aagtgccttg ctccaggtcc aggttcacgt tgtccagaat aacttttccg
     1381 gaataacct
//
LOCUS       DAOMAX010000475         1382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_27682_length_1382_cov_4.127355, whole genome shotgun sequence.
ACCESSION   DAOMAX010000475 DAOMAX010000000
VERSION     DAOMAX010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27682_length_1382_cov_4.127355"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK742_14065"
     CDS             complement(<1..226)
                     /locus_tag="PK742_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HQU77767.1"
                     /translation="MPGILFTHGYFLQDDAKEQQIMRPYPPLGMLYVSAWLTKHGLEN
                     RVFDTTFSTMEKLQEEILSSRPEFLCMYVNL"
     gene            complement(276..1043)
                     /locus_tag="PK742_14070"
     CDS             complement(276..1043)
                     /locus_tag="PK742_14070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQU77768.1"
                     /translation="MQAFDHEVPYYDRQFTDAPIGRLQRKLVWQVLKHYLPDHTMQVL
                     ETNAGTGPDALWLQQAGHRVIPTDASSAMVERMRKKGLDAHIWDINDTVPVELRQQRY
                     DLVFSNFSGWNCVDEQALSALSDELAECTTDKAVVVTVLFGRWVVWEYLYFFFRLQWK
                     QIFRRLRKQTDAFVGGTTIPVWYHRKRDMQTAFDAHFDLIARYPVGSFLPPTYLQPLN
                     RFRGLLKILYLFDTILTRWRICPDTADHTVYVFRRKP"
     gene            complement(1045..>1382)
                     /locus_tag="PK742_14075"
     CDS             complement(1045..>1382)
                     /locus_tag="PK742_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQU77769.1"
                     /translation="NTPQAIAAMNEASALINTAAHTGSGEISARPDTLERMQNTCRTF
                     VYDVFGLKSEDDAGGDVLDGVMNLVIDIRKEARSNKDFATSDKIRDVLSAVGIVLKDS
                     KEGTTWEQS"
BASE COUNT          330 a          362 c          339 g          351 t
ORIGIN      
        1 tcagattgac atacatgcaa aggaattcag gccgggaaga cagtatttct tcctgcagtt
       61 tttccatagt ggaaaaggtg gtatcaaaaa cccggttttc caggccatgt ttcgttagcc
      121 atgccgatac atacagcatg cctaacggcg gatagggccg catgatctgc tgctcctttg
      181 cgtcgtcctg cagaaaatag ccgtgtgtaa agagtatgcc cggcacagcc taaagatagc
      241 agttcggtgc gggcgatggt caatcgtttg tcctgctatg gcttacgacg gaagacatag
      301 accgtatgat cagctgtatc ggggcagata cgccagcggg tcagaatggt atcgaacagg
      361 tataatatct tcagcaaccc ccggaagcga ttcagtggct gcaggtaagt ggggggaaga
      421 aaactgccca ccggataacg ggcgatcagg tcgaaatgtg catcgaatgc tgtctgcata
      481 tctctcttcc ggtgatacca taccgggatg gtggtacctc ccacaaaggc atcggtctgt
      541 ttgcgcagtc ttcgaaagat ctgtttccac tgcagacgaa agaaaaaata caggtattcc
      601 caaaccaccc atcggccgaa caatacggtc accacaacag ctttgtctgt ggtacattcc
      661 gccagttcat ctgagagcgc tgaaagtgct tgctcatcta cacagttcca tccactgaaa
      721 ttcgagaaca ccagatcata tcgctgttgc ctgagttcta caggaaccgt atcgttgatg
      781 tcccagatgt gtgcatccag tcctttcttc cgcatgcgtt ccaccatggc agaagaagca
      841 tccgtaggta tcacccggtg tcctgcctgt tgcaaccaaa gagcgtcggg acctgtacct
      901 gcgttggtct ccagcacctg catcgtatgg tcgggcaaat aatgcttcag cacctgccag
      961 accagctttc gctgcaaacg gcctataggc gcatcggtga attgcctgtc ataataaggg
     1021 acttcgtggt cgaatgcctg catatcagga ttgttcccag gtggtgcctt ccttactgtc
     1081 ctttagcacg ataccaaccg cagagagtac atctctgatc ttgtctgatg tagcaaagtc
     1141 cttgttgctt ctggcttctt tacggatatc aatgaccagg ttcatgacgc cgtccagtac
     1201 atcgccacct gcatcgtctt ccgatttcaa tccgaaaaca tcatatacaa atgttctaca
     1261 ggtattctgc atccgttcca gggtgtctgg ccgagctgat atctctccgc tgccggtatg
     1321 cgctgccgta ttgatcagcg cacttgcttc attcatggcc gctatagcct gcggagtatt
     1381 ga
//
LOCUS       DAOMAX010000476         1380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_27763_length_1380_cov_3.683774, whole genome shotgun sequence.
ACCESSION   DAOMAX010000476 DAOMAX010000000
VERSION     DAOMAX010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27763_length_1380_cov_3.683774"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..765)
                     /locus_tag="PK742_14080"
     CDS             complement(19..765)
                     /locus_tag="PK742_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQU77770.1"
                     /translation="MKIALFSDIHANLPALEAMLADIDAQQPDAIFCLGDLVGYHVWP
                     NEVCELIRERNIPTIAGNYDDGVGRNSDDCGCAYKTDEEKANGARSIAYTNQIITEKN
                     RKFLDELPREIRLPFGEDHARFDLLLVHGSPRRINEYLFEDRTDKSLVHIMEEAEADI
                     LCFGHTHKPWHRTLTGRDGQTLHAINIGSVGKPKDGDPRACYVLLEIDEDGLGEVQFR
                     RVAYDVEGSAAAVEASVLPQAFADALRTAR"
     gene            complement(776..>1380)
                     /locus_tag="PK742_14085"
     CDS             complement(776..>1380)
                     /locus_tag="PK742_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HQU77771.1"
                     /translation="TKGDANYTLVQVSVNDLILLIAFIPIVRLLLGITDIPIPWDVLA
                     ASVVTFVVIPLIFGYLSNRLLLRFKGKEWFKEVFLHRLKPVSIIALLTTLVLLFAFQG
                     DTILNKPVVILLIAIPLTLQTYFIFFLTWFAGRFLKLRHAICAPAAMIGASNFFELAV
                     AVAISLFGLNSGASLATVVGVLIEVPVMLSLVAMANKWRY"
BASE COUNT          338 a          364 c          352 g          326 t
ORIGIN      
        1 catatattga tctgagtact atcgggcagt ccgcaatgca tcggcaaatg cctgtggtaa
       61 aacggatgct tccactgctg cggcagagcc ttccacatca tacgctactc ttctgaactg
      121 cacctcaccc aaaccgtctt catcgatttc cagcaacacg tagcaggctc tcggatcacc
      181 atccttaggc ttccccaccg atccgatgtt gatggcatgg agtgtctgac catctctgcc
      241 ggtcagtgtc cggtgccagg gtttatgggt atgaccgaaa cacaggatgt cggcttcggc
      301 ttcttccatg atgtgtacca ggcttttgtc tgtgcggtct tcaaagaggt attcgttgat
      361 gcgtcgcgga ctcccatgca cgagcaacag atcgaaccgg gcatgatctt caccgaaagg
      421 cagcctgatc tcacggggta attcgtccag gaatttcctg tttttttcgg tgatgatctg
      481 gttggtatag gcgatggacc ttgcaccatt tgccttctct tcatcggtct tgtaggcaca
      541 gccacaatcg tcgctgttgc ggcccacacc atcatcgtag ttgccggcaa tggtgggaat
      601 atttctttca cgaatgagct cgcacacttc attcggccag acatgatagc ccaccagatc
      661 gcccaggcaa aagatggcat cgggttgctg tgcatcgata tcggccagca tcgcttccag
      721 tgcaggcagg ttggcgtgga tgtcggagaa cagggcgatc ttcatgtgtg tagatttaat
      781 atctccattt attcgccatc gccacgagcg acagcatcac aggtacttct atcagtacac
      841 ccaccaccgt agccagcgaa gcaccggagt tcagtccgaa cagtgatatc gccaccgcta
      901 ccgccagctc aaagaaatta ctggcgccga tcatagctgc aggtgcgcat attgcgtgtc
      961 gcaatttcag aaagcgaccg gcgaaccagg tgagaaaaaa gatgaaatag gtctgaaggg
     1021 tgagtggtat ggcgatcagc agaatgacca ccggtttgtt cagaatggta tcgccctgaa
     1081 aagcgaacag caatacaagt gtcgtgagca gggcgatgat ggacaccggt ttcaatctgt
     1141 gcaggaacac ctctttgaac cactcctttc ctttgaaacg cagtagcaga cggttactca
     1201 gataaccgaa gatcaatggt atcaccacaa aggtcaccac cgatgctgcc agtacatccc
     1261 agggaatagg aatatcggtg atgcccagca acaagcgtac gatggggatg aaggctatca
     1321 gcaatatgag atcgttcacc gacacctgca ccagggtgta gttggcatca cctttggtca
//
LOCUS       DAOMAX010000477         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_28202_length_1369_cov_4.264840, whole genome shotgun sequence.
ACCESSION   DAOMAX010000477 DAOMAX010000000
VERSION     DAOMAX010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28202_length_1369_cov_4.264840"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK742_14090"
     CDS             complement(<1..958)
                     /locus_tag="PK742_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQU77772.1"
                     /translation="MMILKIAWRNIWRNRTRSLVLILSIVLGMWAGAFIMAFYWGMSK
                     QQVDDTIQQNLSHIQFHHPDFRENFEAKYTIADGDQVVADLQQDKEVKAYSERVVCMA
                     MAASASKSTGVTLYGIVPEQEDALTGLKEKLIAGDYFTGKKNEVVVGNKLAEKLHLDV
                     RKKIVCTFQDEQGEIVSVALRVSGIFKTRNSRLEEGMIYMQQTDLSALLGTGNGIHEI
                     AVLLADDQQVDAVTDQWKQDFPADDVESWKELSPELRLIVESFNQYMFIIIGIILLAL
                     LFGIINTMLMAILERTKELGVLMSVGLNKTKVFIMILLETVFI"
     gene            complement(960..>1369)
                     /locus_tag="PK742_14095"
     CDS             complement(960..>1369)
                     /locus_tag="PK742_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQU77773.1"
                     /translation="YILYMVIGFGLFSTVLMMLTERQHEFGIMVSIGTGKKQLSRMVF
                     LEILLLSAIGIILGTAASLPILHYLDAHPIRLTGEIADVYASYGFEPVMPVLITPNVF
                     LSQIKTVGLIALLVSLYPIIKLLRFSVMKALRS"
BASE COUNT          321 a          361 c          339 g          348 t
ORIGIN      
        1 cgataaagac cgtttcgagc aggatcatga tgaacacttt cgtcttgttc aatccgaccg
       61 acatcagcac gcccagttct ttcgttcgtt cgagaatggc catcagcatg gtgttgatga
      121 tgccgaacaa cagggcgagc aggatgatgc cgatgatgat gaacatgtac tggttgaagg
      181 actcgacaat gagccgcaac tcgggcgaga gttccttcca gctctccacg tcatcggccg
      241 ggaagtcctg cttccactgg tcggtcaccg catccacctg ctggtcatcg gctaacagga
      301 cagctatctc atgaatgcca ttccccgttc ccagcagtgc agacagatcg gtttgctgca
      361 tgtagatcat cccctcttcc agcctggaat tcctggtctt gaaaatgccg ctcacccgaa
      421 gggctacaga tacaatttca ccctgttcat cctggaaggt gcagacgatc ttcttgcgta
      481 catcgaggtg cagtttttca gcgagcttgt tacccacgac gacctcgttc ttcttgccgg
      541 tgaaataatc gccggcaatg agtttctctt ttaatcctgt cagtgcatcc tcctgttcgg
      601 gtacgatacc atagagtgtt acaccggtac ttttactcgc actggctgcc atcgccatgc
      661 agaccacccg ctcgctgtat gctttcactt ccttgtcctg ttgcagatca gccaccacct
      721 gatcgccatc ggcaatggta tatttcgctt caaaattttc tctgaagtcg ggatggtgga
      781 actgaatatg tgagaggttc tgctgaatgg tatcatccac ctgctgcttg ctcatgcccc
      841 agtagaaggc catgatgaaa gcgccggccc acataccaag tacgatactc aggatgagca
      901 ccaggctgcg ggtcctgttc cgccagatgt ttcgccaggc tattttgagt atcatcatat
      961 caacttctta aggctttcat gacagagaat cggaggagtt tgatgatcgg gtacagactc
     1021 accaataatg cgatcagtcc gaccgtcttg atctgtgaga ggaacacatt cggcgtgatg
     1081 agcaccggca tgacgggttc aaatccgtag ctggcataga catcagcgat ctcgcctgtc
     1141 agccggatgg gatgtgcatc gaggtagtgg aggatgggga ggctggcggc agttccgagt
     1201 atgataccga tggccgataa taataatatc tccaaaaata ccatacgtga cagttgcttc
     1261 ttgcccgtgc cgatggatac catgatgccg aattcgtgtt gtctttcggt cagcatcatc
     1321 agcacggtac tgaacaatcc gaaaccgatc accatataca agatgtaga
//
LOCUS       DAOMAX010000478         1365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_28339_length_1365_cov_3.487786, whole genome shotgun sequence.
ACCESSION   DAOMAX010000478 DAOMAX010000000
VERSION     DAOMAX010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28339_length_1365_cov_3.487786"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK742_14100"
     CDS             <1..191
                     /locus_tag="PK742_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77774.1"
                     /translation="EFVWELDTDDFILDALLFPVGFFLSQSGYDIRINFMGRNRLLVD
                     MDNTGARLLYHFFKPLKH"
     gene            194..568
                     /locus_tag="PK742_14105"
     CDS             194..568
                     /locus_tag="PK742_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HQU77775.1"
                     /translation="MEKTMYENLLHEVTELIRNEVKTETIVGEPFELGEFTCVPVMRL
                     GLGLGAGGGEGTDKNKASGTGGGAGGGFGVDPLGFLVTHNDQINFIPTHGSKGFSAAM
                     EKLPDLLNKFMDMREKEKAVPA"
     gene            632..>1365
                     /locus_tag="PK742_14110"
     CDS             632..>1365
                     /locus_tag="PK742_14110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQU77776.1"
                     /translation="MRSKHSILAGLLLLLPLLVSAQTWKEQEIIVFTDSLLHATYSDT
                     EPGAAVLIARNGKVVHAQGYGLASLELGVANEPDNLFDIGSMSKQFTAVAILQLVDKG
                     LADLDTDVRTYIPEYNTHDHTITLRHLLTHTSGITSFTELDTFATLYNKDMGREEIMD
                     LFMEPELLFTPDNDWSYSNSGFTLAGMIVEKISGQSFPEYMQEHIFQPAGMQHTYIGN
                     LNQVFPKKVSGYSPGEYGWIPAREFS"
BASE COUNT          364 a          317 c          342 g          342 t
ORIGIN      
        1 gtgaattcgt atgggaactc gacacggatg atttcatcct ggatgccttg ctgtttcccg
       61 tcggattctt tctttcacag agcgggtatg acattcggat caatttcatg ggcaggaaca
      121 ggctcctggt tgacatggac aataccggag cccgcctgtt atatcatttc ttcaaacctc
      181 taaaacattg atcatggaaa aaacaatgta tgagaacctc ttacatgagg ttaccgaact
      241 gatcagaaat gaggttaaaa cagagaccat tgtaggtgag ccatttgaac ttggcgaatt
      301 cacctgtgtg ccggtcatgc gactgggtct gggtcttgga gccggtggcg gtgaaggcac
      361 cgacaaaaac aaggcatcag gtaccggagg cggagctggt ggtggtttcg gagtagatcc
      421 tcttgggttc ctggtcaccc ataatgacca gataaacttc attcccacac atggaagcaa
      481 gggcttctca gcagccatgg aaaaactccc tgacctgctg aacaagttca tggacatgcg
      541 tgaaaaagag aaagcagttc ctgcgtaact gctggttggt caattcaggc cccggtgctt
      601 tgcaccgggg cctctttgta tatttacatt catgagatcc aaacattcca tattagccgg
      661 ccttctcctg ttgttgcctc tcctggtttc tgcacagacc tggaaagagc aggagatcat
      721 tgtttttaca gacagcctgt tgcatgccac ctacagcgat accgagccgg gtgcagcagt
      781 tctaattgcc cgaaatggaa aagttgtgca tgcgcaagga tacggactgg catcgctgga
      841 gctgggcgta gccaatgagc ccgataacct gtttgacatc ggctccatga gcaagcaatt
      901 tactgctgtt gccattctgc agctggtgga taaggggctg gcagatctgg atacagatgt
      961 aagaacatac ataccggaat acaacacgca tgaccatact atcacccttc gccatctgct
     1021 gacccacact tcgggcatca ccagctttac cgagctggac acatttgcaa cactatataa
     1081 taaggatatg ggcagggagg agatcatgga tctgtttatg gaacctgaac tgctgttcac
     1141 acctgataac gactggagtt acagcaattc aggtttcacc ctggcaggaa tgatcgtgga
     1201 aaagatcagt ggtcagtctt ttccggaata tatgcaggag catatctttc agcctgcagg
     1261 tatgcaacat acttatatag gtaatctaaa tcaggtgttt ccgaaaaaag tatccggata
     1321 ttcacccgga gaatatggct ggatacccgc aagagaattc agctg
//
LOCUS       DAOMAX010000479         1364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_28379_length_1364_cov_3.343774, whole genome shotgun sequence.
ACCESSION   DAOMAX010000479 DAOMAX010000000
VERSION     DAOMAX010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28379_length_1364_cov_3.343774"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK742_14115"
     CDS             <1..712
                     /locus_tag="PK742_14115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQU77777.1"
                     /translation="TYLIEATPDLPEQMEFLHSRLTFEHAVLPDAIFLTHAHIGHYTG
                     LMYLGREGLGTRNVQVYAMARMKVFLELNGPWSQLVGLHNIELHQLNAGKKTKLSGSL
                     QITPILVPHRDEFSETVGYYIKGKEKTLLFIPDIDKWYRWETDIRELVKEVDYALLDA
                     SFFADGEIPRDMGEIPHPFVVETMALFNDLPEAERNKIWFIHMNHTNPMLYPESDASK
                     EVLQAGYHIARMGDVFEL"
     gene            complement(755..>1364)
                     /locus_tag="PK742_14120"
     CDS             complement(755..>1364)
                     /locus_tag="PK742_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HQU77778.1"
                     /translation="EGTDLTGLKLLIKAKGKCTTDHISMAGPWLKFRGHLDNISNNML
                     IGAVNFFNEQTDNVKNQLTGEYGPVPATQRAYKAAGIGSIVVGDENYGEGSSREHAAM
                     EPRHLGVRAILVKSFARIHETNLKKQGMLALTFADKNDYDKVQEDDSIDIVGLTDFAP
                     GKPLTVVLHHADGSSDSITVNHTYNAQQIGWFKAGSALNLID"
BASE COUNT          335 a          329 c          361 g          339 t
ORIGIN      
        1 aacctatctg atagaagcta ccccggatct gccggaacaa atggaattcc tgcacagccg
       61 gttgacattt gaacatgctg tgctgcccga tgccattttt ctgacacatg cgcacatagg
      121 ccactatacc ggactgatgt acctggggag ggagggattg ggtacacgga atgttcaggt
      181 ctatgccatg gcacggatga aagtttttct ggaactgaac ggtccctggt cgcaactggt
      241 gggcctgcac aacatcgaat tgcatcagct gaatgcggga aaaaaaacaa agttgtcggg
      301 cagcctgcag atcactccca tactcgtgcc gcaccgcgat gagttctctg agaccgtagg
      361 ttattacatc aaagggaaag agaagacctt gttgttcatt ccggatatcg ataaatggta
      421 tcgctgggaa acagacatcc gggaactggt gaaagaagtg gattatgcgc tgctggatgc
      481 gagctttttt gcagacggag agatcccaag agatatgggt gagatcccgc atccgtttgt
      541 tgtggaaacc atggccttat tcaatgacct tcccgaagcc gaacgaaaca agatctggtt
      601 catacatatg aatcatacca accccatgct gtatccggaa agcgatgcaa gcaaagaggt
      661 attgcaagca ggttaccaca tcgcccggat gggagatgtg tttgaactgt aaccgggcac
      721 acatatcctg tgtttccagc tttatacagt gctatcagtc gatcaggttc agtgctgaac
      781 ctgccttgaa ccaacctatc tgctgtgcat tgtaagtgtg gttgaccgtt atgctgtcag
      841 atgaaccatc ggcatgatgc aaaaccactg tgagtggttt gccgggagca aaatctgtca
      901 gtcccacaat gtcgatgctg tcatcttcct gcaccttgtc gtaatcgttc ttatcggcaa
      961 aggtcagtgc cagcataccc tgcttcttca ggttggtctc gtggatccgg gcgaatgact
     1021 tcaccaggat ggcacgaaca cccagatgac gtggttccat ggcagcatgt tcgcgtgaag
     1081 agccttcacc gtaattctca tcgcccacta caatggagcc gatgccggct gctttatagg
     1141 cacgttgggt ggcaggtaca ggaccgtatt cgccggtcag ctggttcttc acattgtcgg
     1201 tctgttcatt gaagaagttg accgcaccaa tgagcatgtt gttggagata ttatcgaggt
     1261 gaccacggaa cttcagccac ggaccggcca tggagatgtg atcggtagta cacttgcctt
     1321 tggctttgat gagcagcttc agtccagtca ggtcagtgcc ttcc
//
LOCUS       DAOMAX010000480         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_28447_length_1362_cov_4.045907, whole genome shotgun sequence.
ACCESSION   DAOMAX010000480 DAOMAX010000000
VERSION     DAOMAX010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28447_length_1362_cov_4.045907"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK742_14125"
     CDS             <1..421
                     /locus_tag="PK742_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HQU77779.1"
                     /translation="VADESAEVIMISAAIESEIAQLDTYEDRQMFLQEIGLEESGLNK
                     LIRSAYHILNLITYFTAGEKEVRAWTITRGMKAPQAAGVIHTDFEKGFIRAEVIAYND
                     YVQYGSEAACKEAGKLRVEGKEYIVQDGDVMHFRFNV"
     gene            complement(425..>1362)
                     /locus_tag="PK742_14130"
     CDS             complement(425..>1362)
                     /locus_tag="PK742_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HQU77780.1"
                     /translation="SKFQQITGIEERRYAVPELNASQIGAIAARLALEDSRVDPETLD
                     QIIVAHNFGDVVKHTIQTDILPALASRIKHELQISNPNCVAYDILFGCPGWVQGVIQA
                     HAFFQSGMARKALVIGTETLSRVLDMYDRDSMIFSDGAGASVLEYKNANHSGVLGYSV
                     QSHCMDEAYYLYMGKSNFPHSDPRVRYIKMHGRKVYEYAMKYVPMAMKDCLDKSGISI
                     DEVKKVFIHQANEKMDEGIIKAFYKLYDRKEIPDLIMPMSIHKLGNSSVATVPTLFDL
                     VRRNELDDHHLEKGDVVLFASVGAGMNINAVCYRV"
BASE COUNT          349 a          329 c          333 g          351 t
ORIGIN      
        1 agtggccgat gaatctgcag aagtgatcat gatatctgct gcaatagagt cagagatagc
       61 gcagctggat acctacgaag accgccagat gttcctgcag gaaataggcc tggaagaatc
      121 cggactgaat aaactcatac gttcagccta tcatatcctg aatctgatca cttatttcac
      181 tgcgggcgag aaggaggttc gagcctggac catcacccgc gggatgaaag caccacaggc
      241 agcaggtgtc atccatacgg actttgaaaa aggattcatc cgtgctgagg ttatcgccta
      301 taatgactac gtgcagtatg gcagtgaagc agcctgcaaa gaggccggta aactgcgggt
      361 agaaggcaaa gaatacattg tacaggacgg agatgtcatg cacttccgct tcaatgtctg
      421 acggtcatac acggtagcac accgcattga tattcatgcc ggcacctact gaagcaaaca
      481 gcaccacatc gcctttttcc aggtggtgat catccagttc atttcgtctt accagatcga
      541 acagggttgg gacagtggct acggaactgt tgcccagctt gtgaatgctc atgggcatga
      601 taagatcagg aatttctttt cgatcataca gcttatagaa tgccttgatg atgccttcat
      661 ccattttttc attcgcctgg tggatgaata ctttcttaac ctcatcaatt gaaataccgc
      721 ttttatcgag gcagtctttc atcgccatag gtacatattt catggcatat tcatacactt
      781 tccttccgtg cattttgatg tagcgaaccc tcgggtctga atgcggaaaa ttggacttgc
      841 ccatatagag ataataagcc tcatccatgc aatgtgattg tacagaataa ccgagcacgc
      901 cactgtggtt tgcattctta tattccaaaa ctgatgcccc tgctccgtcg ctgaagatca
      961 tgctgtcgcg gtcatacata tcgagcacac gagacagtgt ttcagtacct atgacaaggg
     1021 ctttccgggc catacctgac tggaaaaatg cgtgggcctg gatcacaccc tgcacccagc
     1081 cggggcatcc gaacaggatg tcatatgcca cacaattggg gttgctgatc tgcagttcat
     1141 gtttgatgcg gcttgcaaga gcaggaagaa tatctgtctg aatggtgtgt ttgaccacat
     1201 cgccgaagtt gtgtgccacg atgatctgat ccagtgtttc cggatcaact ctactgtctt
     1261 ccagcgcaag tcgggcagcg atggcaccga tctggctggc attcagttca ggtacagcat
     1321 accgacgctc ttcaatgccg gtgatctgct ggaatttgct gg
//
LOCUS       DAOMAX010000481         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_28525_length_1360_cov_3.461303, whole genome shotgun sequence.
ACCESSION   DAOMAX010000481 DAOMAX010000000
VERSION     DAOMAX010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28525_length_1360_cov_3.461303"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PK742_14135"
     CDS             complement(<1..1056)
                     /locus_tag="PK742_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQU77781.1"
                     /translation="MKTNPFRSIGADFPASIVVLLVAIPLCLGIALGSNAPLFSGLIA
                     GIVGGVVIGILSGSQLSVSGPAAGLTVIVAGGIATLPSWEAFLMAVVLAGVFQIIFGS
                     IRAGIIGDYVPNAVIKGMLAAIGIILILKQLPHLVGYDEDFLGDESFQQADHENTFSE
                     LIHMMGALQMGAVLVGILSMLVLIIWERPFMKKMSWSKLVPGPLVAVLVGTFAHLILR
                     NTGSAHSIQPEHLVQIPVASSFTGFIGLFSSPDFSSIALSEVWVLAITLALVASLETL
                     LSIEAVDKIDPHKRITPPNRELLAQGAGNMLSGLIGGLPVTSVIVRSSANVNSGGETK
                     LSAILHGVMLFLCVALIP"
     gene            1111..>1360
                     /locus_tag="PK742_14140"
     CDS             1111..>1360
                     /locus_tag="PK742_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQU77782.1"
                     /translation="MIRAIKPTDNKHLAKLIRDVLTEFGANKPGTVYYDETTDHLSEV
                     FQAAGSRYFVLESSGEILGGAGIFPTDGLPADTCELVKM"
BASE COUNT          371 a          363 c          323 g          303 t
ORIGIN      
        1 gggtatcagc gcaacacaca aaaacagcat gaccccatgc agaatggccg ataatttggt
       61 ctccccaccg gaattgacat tggcagaact acgcacgatc accgaggtga caggaagacc
      121 tccgataagc cccgacagca tatttcctgc accttgcgcc agtagttctc ggtttggcgg
      181 ggtgatgcgt ttatgcgggt ctatcttatc aacagcctcg atactcagga gagtttccag
      241 gctggccacc agtgccagtg tgatggcaag tacccacact tctgaaagtg caatggatga
      301 aaagtctggc gaagaaaaca atccgatgaa cccggtaaag ctacttgcaa caggaatctg
      361 taccagatgt tctggttgaa tgctgtgggc agaacctgta ttacgaagga tcaggtgcgc
      421 aaaagtaccc acgagtacag ccaccagggg gccgggaacc agtttgctcc agctcatctt
      481 cttcatgaaa ggtcgctccc agatgatcag caccagcatg gacagtatgc ctaccaagac
      541 tgctcccatt tgaagggctc ccatcatatg gatcagctct gaaaaagtat tttcatgatc
      601 ggcctgttgg aacgattcat cgcccagaaa gtcctcgtcg taccccacca ggtgtggaag
      661 ctgcttcagg atcagaataa taccaatggc agccagcatt cctttgatca cggcattggg
      721 tacataatcg ccgatgatgc ctgcccggat acttccgaaa atgatctgaa aaacacctgc
      781 cagtaccaca gccatcagaa atgcttccca gctgggaaga gtagctattc ctcctgccac
      841 gatcacggtc agaccggcgg caggcccact tacactcagc tgagaaccac ttaagatccc
      901 gatcactacg cctccaacaa ttcctgctat cagacctgag aacagcgggg cattggaccc
      961 cagcgcaatg cccagacaaa gcgggatagc caccagcaga accactatac tggcgggaaa
     1021 atctgctccg atcgagcgaa aaggattagt tttcatattc ggtcttcaac aaattgttaa
     1081 aggaattgtt taacaacttt tcattttttt atgatccgag ccataaaacc tactgacaac
     1141 aagcatctgg ccaaactgat cagagatgtt ttgacggaat ttggtgccaa taaacccggc
     1201 actgtttatt atgacgaaac caccgatcac ctgtctgagg tgttccaggc tgccggaagc
     1261 cggtatttcg tcctggaatc atcgggcgaa atactgggcg gcgctggtat cttcccaacc
     1321 gatggcctgc ctgctgatac ctgtgaactg gtcaagatgt
//
LOCUS       DAOMAX010000482         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_28633_length_1357_cov_2.944700, whole genome shotgun sequence.
ACCESSION   DAOMAX010000482 DAOMAX010000000
VERSION     DAOMAX010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28633_length_1357_cov_2.944700"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1357)
                     /gene="katG"
                     /locus_tag="PK742_14145"
     CDS             complement(<1..>1357)
                     /gene="katG"
                     /locus_tag="PK742_14145"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQU77783.1"
                     /translation="KTLNLDILHQHDTKTDPYHNAFNYRKAFQELDLEAVKADLKKLM
                     TDSQDWWPADWGHYGGLMIRMAWHVAGTYRSADGRGGANTGNQRFAPLNSWPDNANLD
                     KARRLLWPVKKKYGNKLSWADLMILAGNMAYESMGFKTFGFGGGRADIWHPEKDIYWG
                     AEKEWLGKNRYGSDGDSESLENPLAAVQMGLIYVNPEGVDGKPDPIRTAHDVRTTFKR
                     MGMNDEETVALTAGGHTVGKAHGNGDASALGPMPEAAEVENQGFGWKNPKGSGNAENT
                     VTSGLEGAWTSTPDKWNHTYFHLLLDYDWEVRKSPAGANQWEPVNMPEEEKPFDAHIP
                     GVRRNPIMTDADMAMKMDPEYRKISEKFRQDPAAFEDAFARAWFKLTHRDMGPKSRYL
                     GADVPQEDLIWQDPIPAGNGKLTDAEIDSLKQSILNSGLTASELIATAWDSARTFRTS
                     D"
BASE COUNT          257 a          397 c          351 g          352 t
ORIGIN      
        1 taatcagatg ttctgaaggt acgggcacta tcccatgcag tagcgatcag ctcgctggca
       61 gtcagtccgc tgttcaggat gctctgcttc aggctgtcga tttcggcatc ggtcagtttg
      121 ccgttacctg ccggtatagg atcctgccag atgagatctt cctgcggtac atcggcgccg
      181 aggtagcgac tcttcggtcc catatcacga tgggtcagct tgaaccaggc acgggcaaag
      241 gcatcttcga atgcagccgg atcctggcgg aatttctcag agatcttcct gtattccgga
      301 tccatcttca tcgccatatc agcatcggtc atgatggggt tgcgacgaac gccgggaata
      361 tgcgcatcga aaggtttttc ttcctccggc atattgaccg gttcccactg attggcacct
      421 gccgggctct tcctcacttc ccaatcataa tcgagcagca ggtggaaata ggtatggttc
      481 catttgtcgg gagtactggt ccacgcacct tccagtccgc tggtcaccgt gttctctgca
      541 ttgccgcttc ctttcggatt cttccatccg aaaccctggt tctctacttc ggcggcctca
      601 ggcatcggtc cgagagcgga tgcatcgccg ttaccatgtg ccttacctac agtgtgtccg
      661 ccggctgtca gcgcaacggt ctcttcgtcg ttcataccca tgcgcttgaa ggtggtgcgc
      721 acatcgtgag cggttctgat cgggtctggt tttccatcca ccccttccgg gttcacatag
      781 atgagaccca tctgcacagc tgccagtggg ttctcgagcg attcactatc gccgtctgac
      841 ccatagcgat tcttacccag ccattccttt tcagcgcccc agtaaatgtc tttttcggga
      901 tgccagatat ctgcacggcc accgccaaat ccgaaggtct tgaagcccat ggattcgtaa
      961 gccatgttac cggccaggat catcagatcg gcccaggaaa gtttgttgcc atacttcttt
     1021 ttgaccggcc acagtaacct tctggctttg tccaggttgg cattatcagg ccaggaattg
     1081 agaggcgcaa aacgctgatt acccgtgttg gcaccgccgc gaccatcggc cgaacgatag
     1141 gtacccgcca cgtgccaggc catgcggatc atgagtccgc cgtaatgtcc ccaatccgca
     1201 ggccaccagt cctggctgtc ggtcatcagt ttcttcaggt ctgctttcac cgcttccaga
     1261 tccagctcct gaaacgcttt gcgatagttg aaagcattgt gataaggatc ggtcttggtg
     1321 tcgtgctggt gcaggatgtc caggttcagc gtcttcg
//
LOCUS       DAOMAX010000483         1343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_29225_length_1343_cov_5.130435, whole genome shotgun sequence.
ACCESSION   DAOMAX010000483 DAOMAX010000000
VERSION     DAOMAX010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29225_length_1343_cov_5.130435"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK742_14150"
     CDS             <1..475
                     /locus_tag="PK742_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HQU77784.1"
                     /translation="HLKVVEPLRFLFKDEVRRVGTALSLKPELLNRHPFPGPGLAIRI
                     IGDVTAEKVRILQEADAVYIGHLKRSGWYEQVWQAGAVLLPIQTVGVMGDERTYENVL
                     AIRCVNSTDGMTADWSHLPHELLGKISSDIINHVKGINRVVYDISSKPPATIEWE"
     gene            462..>1343
                     /locus_tag="PK742_14155"
     CDS             462..>1343
                     /locus_tag="PK742_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77785.1"
                     /translation="MNGNKHILILLLSLFLLPSCAFLQDLFGEPEPEKPPVVIDKHPE
                     EKEDTLVIDEPQEKEDHIKDKKDEQKGKWAKRDVYRISYILPFHLDRTELENLMSQDN
                     ITGMQSLASLEFYEGSLMALDTLQEMGLNLEVSLFDDLRDVGRLEDILDQYEMIQSDV
                     VIGPLFNSSLEVASEWAKANEKFIVSPLSPSSSAAKENPWFLMANSTLGTQLQTLLLS
                     WTKQSVNYRIIIASKGETGTDSLIKAEVIKAWDALKVDGYKPQLAFANSAEAVQNSMI
                     QGRDNFVFLASTDELFVN"
BASE COUNT          360 a          325 c          361 g          297 t
ORIGIN      
        1 gcacctgaag gtggtggaac cgctgcgttt ccttttcaag gacgaggtgc gcagagtggg
       61 tacagccctc agcctcaagc cggagctgct gaacagacac cctttccccg gaccgggact
      121 ggccattcgc atcataggcg atgtgaccgc agaaaaagtg cgcatcctgc aggaagctga
      181 tgctgtgtat ataggccacc tgaaaagatc cggctggtat gaacaggtat ggcaggccgg
      241 agccgtgctg ctgcctatac agaccgtggg tgtgatgggt gatgaaagaa catatgaaaa
      301 cgtgctggcc atccgttgtg taaattcgac cgatggcatg accgccgact ggagtcacct
      361 gccccacgaa ctgctgggaa agatcagctc cgatattatt aaccatgtga aagggatcaa
      421 ccgtgttgtt tacgatatca gctccaaacc gcctgcaacc attgaatggg aataagcata
      481 tactaatcct cctgctctct ctgttcctgc tgccctcctg cgcctttctg caggacctgt
      541 tcggcgaacc ggaaccggaa aaacctcctg tggtgatcga taaacacccg gaggaaaaag
      601 aagatacact tgtcattgat gaacctcagg aaaaggaaga ccacatcaaa gacaagaaag
      661 acgaacagaa gggaaaatgg gctaaacgtg atgtgtaccg tatatcctat atcctgccct
      721 ttcacctgga ccggaccgag ctggagaacc tgatgagtca ggacaatatt accggtatgc
      781 agagtctggc cagcctggaa ttctatgaag gaagcctgat ggctctggat acgctgcagg
      841 agatgggact gaatctggag gtctccctgt tcgatgacct gcgtgatgtg ggcagactag
      901 aagacattct ggatcaatac gaaatgatcc agtctgatgt ggttatcggt ccgctgttca
      961 acagcagtct ggaagtagcc agcgaatggg cgaaagccaa tgagaagttc attgtttctc
     1021 cgttgtcgcc ttcatcatct gcagcaaaag aaaacccctg gtttctgatg gccaattcta
     1081 cactgggcac tcagctacag actttgttgt tgtcctggac gaagcaaagt gtcaactatc
     1141 gtatcattat cgccagcaaa ggtgaaaccg gtaccgacag tctgatcaaa gcagaagtga
     1201 tcaaagcatg ggatgccctg aaggtggatg gatacaagcc gcaactggcc tttgccaaca
     1261 gtgcggaagc agtgcagaac agcatgatac agggtcgtga caattttgtt ttcctggcat
     1321 cgaccgatga gctgttcgtg aac
//
LOCUS       DAOMAX010000484         1341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_29313_length_1341_cov_4.429238, whole genome shotgun sequence.
ACCESSION   DAOMAX010000484 DAOMAX010000000
VERSION     DAOMAX010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29313_length_1341_cov_4.429238"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..>1341
                     /locus_tag="PK742_14160"
     CDS             280..>1341
                     /locus_tag="PK742_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQU77786.1"
                     /translation="MIRDIHFNGSAGNTVRLEESEDLFIVRTREHIPALNLLSLWPEL
                     QDILPLITPFDAFPESSVWLYQIHHEQPAEVRRLIKDLVRAKSDPLIRFIGSVWVFAG
                     SDMYQVYTGNLFIKFANDAGGQHMQDFLELHTLQKKMTLGFATNAFFVEAAEMVGRDI
                     FTLSEALLQEELVTYCHPELVARRKSVFKNELPALPVSDEQNNNWIRQMVGLEEAWQY
                     STGTGVKICVIDDGLDFLHPAFATPEKIAAYRDMLDKQGMRKPLHQFEEKHGTACAGV
                     ACSADTRAPGVAPDAQIIPIRSMGLGSVLESEAIYWAVQQGADIFSCSWGPPDGDFTR
                     KEKATFTYPLPDHTRLALEY"
BASE COUNT          338 a          334 c          344 g          325 t
ORIGIN      
        1 acggtcaggc agatatcagc cacctgcccc ccggcattta tctcaccacc gtacataccc
       61 ggcaaggcat atggagagag aaatgggtga agttgtaaga agaccaggtt aaaatagggt
      121 gaaaacaccc tgttctcgtt ggctgatctt tgaaaacttt gtaccttata ctgcagatgc
      181 ttaccccata cgctgtctga actgtgctac ctgctttgtg aggtcttatt ttcttcaggt
      241 tgttgtatgc cggtaagctg tccttctgaa ggatagctga tgatcaggga tatacacttt
      301 aacggttcag ccggaaatac ggtcagactc gaagagtctg aagatctgtt catcgtcaga
      361 acacgtgagc acattcctgc actgaacctt ctcagcctct ggcctgagtt gcaggacata
      421 ctgccgctca tcacgccatt tgatgccttt ccggaatctt ccgtctggtt gtatcagata
      481 caccatgaac aaccggccga agtgcgccgg cttatcaaag acctggtcag ggcaaagagc
      541 gacccgctca tccgcttcat aggttccgtg tgggtcttcg ccggcagtga tatgtatcag
      601 gtctataccg gaaacctgtt catcaaattt gccaatgatg ccggcgggca gcacatgcag
      661 gactttctgg aattacatac cctgcaaaag aaaatgacac tgggttttgc aaccaatgcc
      721 ttctttgtgg aagctgctga gatggtgggc cgcgacattt ttaccctgag tgaagccttg
      781 ctgcaagagg aactggtaac gtattgccat cctgaactgg tggcaagaag aaaatctgtt
      841 ttcaagaatg aactgccggc actgccggta tccgatgaac agaataataa ctggatcaga
      901 cagatggtcg ggctggaaga ggcctggcaa tacagcacag gtacaggcgt gaagatctgt
      961 gtcattgacg acggactgga ttttctgcat cctgcatttg ctacaccgga aaagatagcg
     1021 gcctacagag atatgctgga caaacagggc atgcgcaaac cacttcacca gttcgaagaa
     1081 aaacacggca ccgcttgtgc aggtgtggca tgctcggcag atacccgggc tccgggtgtg
     1141 gcacccgatg cacagatcat tcccatccgt tccatgggtt tggggtcagt gttggaatct
     1201 gaagccatct actgggcggt gcaacaggga gcagatattt tttcctgttc ctggggtccg
     1261 ccagacggcg attttacccg gaaagaaaaa gccactttta cctacccgct accagaccat
     1321 accagactcg ctctggagta t
//
LOCUS       DAOMAX010000485         1339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_29395_length_1339_cov_3.705607, whole genome shotgun sequence.
ACCESSION   DAOMAX010000485 DAOMAX010000000
VERSION     DAOMAX010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29395_length_1339_cov_3.705607"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1339
                     /locus_tag="PK742_14165"
     CDS             <1..>1339
                     /locus_tag="PK742_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HQU77787.1"
                     /translation="IGSGPYIEVAYDSTLDLPDGGYILVIAPEGIKVTGKADGGVFHG
                     LMSLLQLMEPKPDRTISIPCQTISDGPRFVWRGMHLDVCRHFFSVDEVKQYIDLLALY
                     KFNSFHWHLTDDQGWRIEIKQYPKLTEVGGWRNGTMVGRYSDQQYDSIRYGGYYTQEE
                     IREVVDYAAKRHINIVPEIEMPGHATAALAAYPEFSCTGGPFEVAQGWGVFPDIFCPT
                     EETFTFLENILDEVMALFPSSVIHIGGDEAPKDRWKECAHCQALMQREGLASEEELQS
                     YFVQRIEKYVNSKGRTIIGWDEILEGGLAPNAMVMSWRGTEGGIAAAKDKHQVVMSPN
                     NDCYFDHYQGDPANEPLAIGGMTTLEDVYNYEPVPPGLSKEESAFIIGAQANVWTEYI
                     LNFEHVEYMAVPRMIALSEVLWSTNRKSNFDDFKLRLARNLQLLDRQHVNYSRT"
BASE COUNT          348 a          316 c          374 g          301 t
ORIGIN      
        1 gatcggttca ggcccttaca tagaagtggc ctacgacagc accctggacc tgccggatgg
       61 aggatatatt ctggtaattg ctcctgaagg gatcaaagta acgggtaaag cagatggtgg
      121 cgtattccat ggcctgatgt ctctgctgca gctcatggag ccgaaaccgg atcgcaccat
      181 ttccattcct tgtcagacca tcagtgatgg ccctaggttt gtctggcggg gtatgcacct
      241 tgatgtatgc cggcatttct tttcggtgga tgaggtcaaa cagtatattg atctgcttgc
      301 cctgtataag ttcaattcct tccactggca tctgaccgac gatcagggct ggcgaataga
      361 gatcaaacag tatccgaagc taactgaagt gggtggatgg cggaatggta ccatggtagg
      421 ccggtacagc gatcagcaat atgacagcat tcggtatggt ggctactaca cccaggagga
      481 gatcagagag gtggtggatt atgctgccaa aaggcatatc aacatcgtgc cggagatcga
      541 aatgcccgga catgccaccg ctgcactggc agcttatccg gaattctcct gtacaggtgg
      601 tccgttcgaa gtggctcagg gctggggtgt tttcccggat atcttttgtc cgaccgaaga
      661 gacattcact ttcctggaaa atatcctgga tgaagtgatg gcactcttcc cctcttccgt
      721 cattcatatt ggcggcgatg aagcccccaa agaccgctgg aaagaatgtg cgcattgtca
      781 ggcgctcatg cagcgggaag gacttgccag cgaagaagag ctgcagagct actttgtgca
      841 gcgcatcgaa aaatacgtga acagcaaagg gcgcaccatc ataggctggg atgagatcct
      901 ggaaggcgga ctggcaccga atgccatggt catgagctgg aggggaacag aaggtggtat
      961 agctgcagcg aaagacaaac accaggtggt aatgagtccg aacaacgatt gttatttcga
     1021 ccattaccag ggcgatcctg ccaacgagcc gctggccatc gggggtatga caacactgga
     1081 agatgtatat aattatgagc cggtaccacc tggcttaagt aaagaagaat ccgccttcat
     1141 catcggtgca caggccaatg tctggaccga atacattctg aactttgagc acgtggaata
     1201 tatggccgtt ccgaggatga tcgccttgtc tgaagtgctg tggtcaacaa acagaaaaag
     1261 caattttgac gacttcaaac tccggcttgc acggaacctg caactactgg acaggcagca
     1321 cgtgaattac agcagaacc
//
LOCUS       DAOMAX010000486         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_29605_length_1334_cov_3.890539, whole genome shotgun sequence.
ACCESSION   DAOMAX010000486 DAOMAX010000000
VERSION     DAOMAX010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29605_length_1334_cov_3.890539"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PK742_14170"
     CDS             <1..916
                     /locus_tag="PK742_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77788.1"
                     /translation="IVLLSACTRQQASDELRTINPVIGDQSYLAMFGEEPDAGLSEHD
                     RIAIHLAYVEQLLRQKDVSGLSAEERIQRSEMLDLLHEYREGRQYPSNYDHPEGRRPC
                     FIDRDGNICAVGYLVEQSAGRELAEQINAKYQYDYIRDMDLPELADWVAGSGLSLEEV
                     AMIQPTYGYYPPVSYIEPSYGISSALLGGVNMSLSALNSIQMIKGQSSLALGILGVAG
                     GTGQVIHGFLNMPEEIVYFNAPPDTNEGQKVLSLVNIGIGTTTTILSAYQLLSNCHKK
                     DRSTTWNVFSYPTGTEQAGVGIAMVKRF"
     gene            1088..>1334
                     /locus_tag="PK742_14175"
     CDS             1088..>1334
                     /locus_tag="PK742_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77789.1"
                     /translation="MITEKDRQIIDFIRKNAGSSSKYVFENAAVSVSYATLKRMLTKL
                     VAERCIVIEGQGKGTRYFLSPSFELVQPISIDEYYEKE"
BASE COUNT          373 a          292 c          350 g          319 t
ORIGIN      
        1 cattgtgctg ctgagtgcct gcacaaggca acaggcttcg gatgaactcc gcaccatcaa
       61 ccctgtgatc ggagatcaga gttatctggc catgttcgga gaagaacctg atgcaggcct
      121 aagcgagcat gaccgcatcg ccatccatct ggcatatgtg gagcagctgc tcagacagaa
      181 agatgtgagc gggttatcgg cagaagaaag aatacagcgc agtgagatgc tggacctgct
      241 gcacgagtac cgggaaggca ggcagtatcc gtccaactac gatcacccgg agggaagaag
      301 accctgtttc atagaccgtg acggcaatat ctgtgccgtg ggttacctgg tggaacagag
      361 cgccggaaga gagctggcag agcagatcaa tgcaaagtac cagtacgact atatccgaga
      421 catggacctg cctgaactgg ccgattgggt agcgggcagc ggactcagcc tcgaagaggt
      481 ggccatgata caaccaacct atgggtatta cccacctgta agctatatcg aaccttcata
      541 tggcatttcc tctgctttgc tgggtggggt gaacatgtca ctgtcggcct tgaacagcat
      601 tcaaatgatc aaaggacagt cctccctggc attgggtatt cttggggttg ccggagggac
      661 ggggcaggtc atccatggat tcctgaacat gccggaggaa attgtctatt tcaatgcacc
      721 ccctgatacc aatgaaggac agaaagtact cagcctggtc aacataggca tcggcaccac
      781 cactaccatt ctgagtgctt atcagttgct gtccaattgc cataaaaaag acaggtcaac
      841 cacctggaac gtattcagct atccaaccgg aacagagcag gcgggtgtgg gtattgcgat
      901 ggtgaagagg ttctgatgtt cgcattctgt cttgccgtgt gaaggccatg aaacaaggaa
      961 cgtagctgac ttttaccgac tctttatgcc tggacgggtc tttgtagctc attttatcat
     1021 ggaatactgt tcaattttac actaatttga gcttgaaata gtattttagt gagcttgaaa
     1081 ttatcttatg ataacagaga aagaccggca aataattgac tttattagaa agaacgcagg
     1141 tagttcgtct aaatacgttt tcgagaacgc tgctgtatct gtgagttatg caaccttgaa
     1201 acggatgtta acaaagctgg ttgctgagag atgcatagtt attgaaggac agggaaaagg
     1261 aaccagatac tttctttcac catcatttga actggtgcaa ccaataagca tagatgagta
     1321 ttatgaaaaa gaga
//
LOCUS       DAOMAX010000487         1328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_29886_length_1328_cov_3.712490, whole genome shotgun sequence.
ACCESSION   DAOMAX010000487 DAOMAX010000000
VERSION     DAOMAX010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29886_length_1328_cov_3.712490"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1328)
                     /locus_tag="PK742_14180"
     CDS             complement(<1..>1328)
                     /locus_tag="PK742_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQU77790.1"
                     /translation="FQQLVALGQHPKEFTDTITVLEKLGHFLDREVQQLFDQYKPYHE
                     NQKISELIASELNVQNVLARAARDFEGGYAIMGMLGHGDAFLLRDPNGIRPAYYYHDD
                     EVVVAASERPAIMTAFDVPFSQVKELTPGHALIIKKKGTVKEQLCRQPLEKKSCSFER
                     IYFSRGGDQEIYEERKMLGRLIAPKVLEAVHYDYDHTIFSFIPNTAEVAFYGMMKGLE
                     DHLKEIKKKKLLELEDKTDATAVESILSMRPRVEKLAIKDVKLRTFITNDDDRSNMVS
                     HVYDVTYGQVESHVDTIVLIDDSIVRGTTLKRSIIAILDRLEPKKIVIVSSAPQIRYP
                     DCYGIDMSKMGDFVAFKAAIELTQERGNAALLDEVYKECREMEHWPSEKVHNAVQKVY
                     AQFTDEEISAQVSKIVKAPHIRAEVQVMYQSVDDLHTACPHNLGDWYFTG"
BASE COUNT          277 a          354 c          332 g          365 t
ORIGIN      
        1 tgccggtgaa gtaccagtcg ccgaggttgt gcggacaggc ggtatgcaga tcatccacgc
       61 tctggtacat gacctgtacc tcagcacgga tatgcggggc cttgacgatc ttacttacct
      121 gggcagagat ctcttcatcg gtgaactgcg catagacctt ctgcacggcg ttgtgcactt
      181 tctctgacgg ccagtgctcc atctccctgc actctttata aacctcatcc agcagagcgg
      241 cattacctct ttcctgtgtc agttcgatgg ccgccttgaa ggcgacaaaa tcacccatct
      301 tgctcatgtc tatgccatag caatcgggat agcggatctg cggtgcggag gatacgatga
      361 cgatcttttt cggttcgagg cgatcgagga tggcgatgat gctgcgcttc agtgtggtac
      421 cccgaacgat ggaatcgtca atgagtacga tggtatccac atggctttcc acctgtccgt
      481 aggtcacgtc atacacgtgg ctcaccatgt tgcttcggtc atcgtcgttg gtaatgaagg
      541 tgcgcagttt cacatccttg atggcgagtt tctccaccct ggggcgcatg ctcaggatgc
      601 tttccacggc tgttgcatcc gtcttgtctt ccagctccag cagtttcttt ttcttgatct
      661 ccttcaggtg gtcttccagt cctttcatca taccgtaaaa cgctacttct gccgtattgg
      721 gaatgaatga aaagatggta tggtcgtagt cataatgaac ggcttccagc accttgggag
      781 cgatgagtct tcccagcatc tttcgttctt cgtagatctc ctgatcgccg ccacgactaa
      841 agtagatacg ctcaaaactg caggactttt tttccagtgg ctggcgacaa agctgctcct
      901 ttaccgtgcc tttcttcttg atgatcagtg catgtccggg tgtcagttct ttcacctggc
      961 tgaagggtac atcgaaagcc gtcatgatgg ccggacgttc gctggcggct accactactt
     1021 catcgtcatg ataataataa gccggacgaa tgccattcgg atcacgcagt aaaaaagcat
     1081 cgccgtgacc cagcataccc atgatggcat atccgccttc aaaatctctt gcagcacgtg
     1141 caagtacgtt ctgcacgttc agttcagaag cgatcagctc actgatcttc tggttctcgt
     1201 gatagggttt gtactgatcg aacagttgct gtacttcacg gtccaggaaa tgcccgagtt
     1261 tttccagcac ggtgatggta tcggtgaatt ccttagggtg ctgacccaat gctaccagct
     1321 gctggaac
//
LOCUS       DAOMAX010000488         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_30028_length_1325_cov_2.842520, whole genome shotgun sequence.
ACCESSION   DAOMAX010000488 DAOMAX010000000
VERSION     DAOMAX010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30028_length_1325_cov_2.842520"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1325
                     /locus_tag="PK742_14185"
     CDS             153..>1325
                     /locus_tag="PK742_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQU77791.1"
                     /translation="MIQLLHKRIILFSAILLTGLLQAQAQNAQRYWVYFQDKEGSGYT
                     LEHANEFLSRASLERRERQGIALTNSDLPVSGEYLQQIAQPGVQIRVVSRWLNAASVE
                     ASPEQLAMFATLPFVKKIEPVRTWRQEEEKVTDLSTFRQAADETSPFGQATVQNSMIG
                     LEFLHDMGYRGENMTIAVLDGGFAGVNEGPGFAKFFENEQILGTWNFPDANEDVYFSS
                     LHGSYVLSIIGAELPGTYLGASPDAGFYLLRTEVVDSERVAEEDFWLAGAEYADFIGA
                     DIINSSLGYTTFDEPSEDHTYADMDGNTTVATIAADMAASKGILVCNSAGNSGNDDWT
                     YIGAPADGDSVFSIGAVDANRQYAFFSSRGPTFDGRVKPNISVMGENSAIVDPSGIV"
BASE COUNT          302 a          349 c          388 g          286 t
ORIGIN      
        1 aagcgtgggg atggtgtaca ggtacttccg ccacttcacc acccagacca tctgccccga
       61 ttgcccctgg gaggataatg tagaatgcgg ctatagtgtg accatgacgg tcattgatca
      121 taattaattg tcaatgaatt aaatagatgg tattgattca actattacat aaaagaatta
      181 tcctgttttc ggccatcctg ttgacaggct tgctgcaggc tcaggcacag aacgcccagc
      241 gctactgggt gtattttcag gacaaggaag gatcgggtta taccctcgaa catgccaacg
      301 aatttctgag ccgggcctct ctggaaagac gggagcgcca gggcatcgca ctgaccaatt
      361 cagacctgcc tgtctcgggc gagtacctgc agcagatcgc ccagccgggt gtccaaatca
      421 gggtcgtttc caggtggctg aatgccgcca gcgtggaagc atcgccggag cagctggcca
      481 tgtttgccac cttgcccttt gtgaaaaaga tagagccggt gcgaacctgg aggcaggagg
      541 aagaaaaagt gacagactta tccaccttcc ggcaggctgc tgatgagacg agtcccttcg
      601 gtcaggccac cgtgcagaac agcatgatag gcctggaatt cctgcatgac atgggctacc
      661 ggggcgaaaa tatgaccatc gccgtgctgg atggcggatt tgcgggcgtg aatgagggtc
      721 cgggtttcgc caagttcttc gaaaatgaac agatcctggg cacctggaac ttccccgatg
      781 ccaatgaaga cgtctatttc tccagcctcc acgggtcgta tgtgctgagc atcatagggg
      841 cagagctgcc cggaacctac ctcggggctt ctcctgacgc cggtttctac ctgctgcgca
      901 ccgaagtggt ggacagtgag cgggtggcgg aagaggattt ctggctggca ggcgccgaat
      961 acgccgattt tataggggcg gatatcatca atagttcttt gggttatacc accttcgacg
     1021 agccgtcgga agaccatacc tatgccgata tggacgggaa taccaccgtg gcgacgatag
     1081 cagccgatat ggcggccagc aagggcatcc tggtgtgcaa cagtgcgggc aactccggca
     1141 acgacgactg gacctatatc ggtgctccgg ccgatggtga ttcggtgttc tccatcggtg
     1201 ccgtggatgc caatcgccaa tacgcctttt tcagctccag aggtcccacc ttcgacggac
     1261 gggtcaagcc caatatctcc gtgatgggag agaacagcgc catcgtcgat ccgagtggga
     1321 tcgtt
//
LOCUS       DAOMAX010000489         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_30033_length_1325_cov_2.707874, whole genome shotgun sequence.
ACCESSION   DAOMAX010000489 DAOMAX010000000
VERSION     DAOMAX010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30033_length_1325_cov_2.707874"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1325)
                     /locus_tag="PK742_14190"
     CDS             complement(<1..>1325)
                     /locus_tag="PK742_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQU77792.1"
                     /translation="KVRTDTEKFMSAFVNVLFPAFVSIVFVSTVAARIHWSLPFIYFG
                     GIALLTLITNMLSKQVKTIQKKIVGQTTSLAGATTESLRNIELVKSLGLTGQEVDRLN
                     QNTYKILGLELTKVKRIRSISFIQGTFVNTLRQVILFMLLWLIFRGEMNAGQLVMMQI
                     FSFFVFGPLQEIGNVILSYREAEASLNNFRTLMDKEPEPEADDPLPLGKVETLDFEQV
                     KFKHKTAGTNAIDGISFHAKSGETIAFVGPSGSGKSTLMKLLVGLYRPQNGRILYNGK
                     DENSIDFNQLRNQIGFVTQDTQLFAGTIRENLLFVNPEASEEDLMDVLSKASCMKLLQ
                     RAEKGLDTMIGEGGLKLSGGEKQRLSIARALLRKPTLLIFDEATSALDSLTEEEITAT
                     IRDVSAERSHITILIAHRLSTIMHADRIYVLEQGEVIETGTHEKLLEQTG"
BASE COUNT          279 a          340 c          359 g          347 t
ORIGIN      
        1 cctgtctgct ccaggagttt ttcgtgtgtt ccggtttcta tcacctctcc ctgctccagc
       61 acgtatatcc ggtcagcatg catgatggta ctcagcctgt gggcgatgag gatggtgatg
      121 tggctgcgct ctgcagatac gtcgcggatg gtggctgtga tctcttcttc ggtcagacta
      181 tcgagggcac tggtggcttc gtcgaagatg agcagggtag gtttgcgcaa cagggcacgg
      241 gcgatggaca gtcgttgttt ttctccgccc gatagtttca gtccgccttc accgatcatg
      301 gtatccaggc ctttttctgc tctctgcaac agcttcatac aactggcttt gctaagcaca
      361 tccatcaggt cttcttcaga agcttccggg ttgacgaaca agaggttctc gcggatggtg
      421 ccggcaaaca actgcgtgtc ctgggtaaca aaaccgatct ggttgcgaag ctgattgaaa
      481 tctatgctgt tctcgtcttt tccattgtag agaatgcgac cattctgcgg acggtacaaa
      541 cccaccagca atttcatcag cgtactctta ccggaacctg acggacccac aaaggcgatg
      601 gtctctccgc ttttggcatg gaaagaaata ccgtcaatgg cattggtgcc ggctgtcttg
      661 tgtttgaact tcacctgttc aaaatcaagg gtttccactt tgcccagtgg cagcgggtca
      721 tcggcttccg gctccggttc tttatccatc agggtgcgga aattgttcag cgatgcttcg
      781 gcttcgcggt aggacaggat cacgttgccg atctcctgca gcggcccgaa cacgaagaag
      841 ctgaagatct gcatcatgac cagctgtccg gcattcattt cgccccggaa gatcagccat
      901 aacagcatga acagaatgac ctgcctgagg gtgttgacaa aagtgccctg gatgaaactg
      961 atgcttcgga tacgcttgac cttggtgagt tcgaggccga ggatcttata ggtgttctgg
     1021 ttcagacggt ccacttcctg tccggtaagt cccaggcttt tcaccagttc gatgttgcgc
     1081 agtgattcgg tggtggcacc cgccagcgat gtggtctgtc ccacgatctt tttctggatg
     1141 gtcttgacct gcttactcag catattggtg atgagggtga gcaatgcaat gccaccaaaa
     1201 tagatgaagg gcagcgacca gtgtatgcgg gcagcgacgg tacttacgaa tactatgctc
     1261 acaaacgcag ggaacagcac attcacaaag gcactcatga atttttcggt gtcggtgcgc
     1321 acctt
//
LOCUS       DAOMAX010000490         1322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_30149_length_1322_cov_3.102605, whole genome shotgun sequence.
ACCESSION   DAOMAX010000490 DAOMAX010000000
VERSION     DAOMAX010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30149_length_1322_cov_3.102605"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1322
                     /locus_tag="PK742_14195"
     CDS             <1..>1322
                     /locus_tag="PK742_14195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HQU77793.1"
                     /translation="PTEIQFIDGANSIWRQIGVWSHYAYVANESGGGILCIDLENLPI
                     TGVADFAFSDGGIGITTSHILYCDEAGTIYVFGSNKHSGSTVMLDATADPMDPPYLGA
                     TDDWYVHHGFARNDTLYESNIYEGWFSVWDVSDKAAPVLLATQETPGVFTHNVVAMDN
                     GIHLFSTDEVTNGSLGAYDISDLSDIQYKGEFRPTPGTNSIPHYTWNKEHWLTTAWYR
                     DGLVITDNSHPEAIVMTGWYDTSPLSGNGFNGAWGVYPYFPSGNVVVSDIEEGLFVLG
                     VDYVSAAFLSGTVTDASTSAPLFGVSVELSDPSGSTSTALDGGYITGLATAGTYSATY
                     SKPGYITATVTGIVLENGVTTIEDVELEPLASFPFNGQVLDVVTGIGVPGANVLVQNA
                     DVSFEATTDGTGLFVIPAIFEGTYSVYAGKWGYVTNAEIDLDINIGSE"
BASE COUNT          313 a          328 c          344 g          337 t
ORIGIN      
        1 atcccaccga gattcagttc attgacggag ctaacagcat ctggcgacag atcggtgtgt
       61 ggagtcatta tgcctatgtg gccaatgaat ccggcggagg gatcctctgc atcgatctgg
      121 agaacctgcc catcaccggt gtggctgact ttgctttcag cgatggtggc ataggcatca
      181 ccacttccca catcctgtac tgcgatgagg ccggcactat atacgtgttc ggttccaaca
      241 agcacagcgg aagtacggtc atgctggatg ccactgctga tccgatggat ccgccttacc
      301 tcggtgctac cgatgattgg tatgtgcacc atggttttgc ccgcaacgat accttatacg
      361 aatcaaacat ctatgaaggt tggttctccg tctgggatgt ttcagataaa gccgctccgg
      421 tgttacttgc tacccaggaa actccgggtg tgttcaccca taacgtggtg gcaatggata
      481 atggcatcca cctgttcagc accgatgagg tgaccaacgg atcgctggga gcatacgata
      541 tcagcgacct gtcagacatc cagtacaaag gtgaatttcg cccgacgccg ggtaccaact
      601 ccatacctca ctatacctgg aacaaggaac actggcttac caccgcctgg taccgcgatg
      661 gtctggtcat taccgataat tctcatccgg aagccattgt tatgaccggc tggtatgata
      721 cctctccact atcaggaaac ggttttaacg gtgcctgggg tgtgtatcca tatttcccct
      781 ctggtaacgt agtggtatct gatattgaag aaggactttt tgtgctgggg gtggactatg
      841 taagtgcggc attccttagc gggacagtta cagatgcttc cacttcggca cccctgtttg
      901 gagtcagtgt tgaactgtca gatccttcag gatctacttc aacagcactg gatggcgggt
      961 atataaccgg acttgctaca gcgggcacct acagtgcaac atattccaaa cccggatata
     1021 ttactgccac cgttactggt attgtcctgg aaaacggagt aaccaccata gaggatgtag
     1081 agctggaacc actggcgagc ttcccgttca acggacaggt gcttgatgta gttaccggca
     1141 ttggggtgcc cggtgccaat gtgctggtgc aaaatgctga tgtctctttt gaggcaacaa
     1201 cagacggaac aggtcttttt gttataccgg ccatttttga aggaacatac agcgtttatg
     1261 ccggcaaatg gggttatgtg accaatgctg aaattgacct cgatatcaat atcgggtcgg
     1321 aa
//
LOCUS       DAOMAX010000491         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_30188_length_1321_cov_3.896524, whole genome shotgun sequence.
ACCESSION   DAOMAX010000491 DAOMAX010000000
VERSION     DAOMAX010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30188_length_1321_cov_3.896524"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PK742_14200"
     CDS             complement(<1..330)
                     /locus_tag="PK742_14200"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQU77794.1"
                     /translation="MRECENVHFITVIGYNNSTQVQGFGFLEEDQAPYNTGNVAVDLS
                     LKLALETISFCELLDRSGKTTISYQFLRSGTSVGANISEAQEAESRADFIHKIKIAAK
                     ETREVMYW"
     gene            complement(354..>1321)
                     /locus_tag="PK742_14205"
     CDS             complement(354..>1321)
                     /locus_tag="PK742_14205"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HQU77795.1"
                     /translation="VVLSKQLKPRSLKGFNIQPEDDYLKSRKAVLVNNDLHISLAAPK
                     KSMTDYFYKNTDADEMIFIHEGSGKLLSAYGEVSFEYGDYVIIPRGTVYQLHFNDENN
                     RLLIVESFSPIDFPKRYMNKVGQLLEHSPFCERDIKLPVNLQTHDETGDFLFYIKKQG
                     IMYPYHYMHHPFDLIGWDGYVYPYAFSIHNFEPITGRVHMPPPIHQTFEAHNFVVCSF
                     VPRMYDYHPLAIPAPYNHSNIDSDEVLYYVDGDFMSRKSVERGQITLHPGGIPHGPHP
                     GTYEGSIGKAETKELAVMIDPFRPLQLTEAAMGIEDEGYYLSWLE"
BASE COUNT          331 a          311 c          280 g          399 t
ORIGIN      
        1 ccaatacatc acctctctgg tttctttggc tgctattttg attttatgaa taaagtctgc
       61 cctgctttca gcttcctgag cttcagaaat atttgcacct acagatgtac ctgatctcag
      121 aaattggtat gaaattgttg ttttaccgga tctatccaat agttcacaaa atgagatggt
      181 ttccaatgca agttttaaac taagatcaac agctacattt ccggtattgt aaggtgcctg
      241 atcttcttcc agaaaaccaa atccttgaac ttgagttgaa ttattgtagc cgattaccgt
      301 tataaaatgc acattttcac attctcgcat ttccacattt tctaattagc taattattca
      361 agccaactca gataataccc ctcatcctca attcccattg ctgcttctgt gagttgtaat
      421 ggtctgaacg gatcgatcat gaccgccagt tctttggttt cggctttgcc tatacttcct
      481 tcataggtgc ccggatgtgg tccgtgcgga ataccacccg gatgcaatgt gatctgcccg
      541 cgttccacac tcttgcggct catgaagtcg ccatccacat agtataacac ctcgtcgctg
      601 tcaatattgc tgtggttgta aggtgcaggt atggccagcg gatgatagtc atacatcctc
      661 ggtacaaatg aacagactac aaaattatgt gcttcaaagg tctggtgaat gggaggcggc
      721 atatgcaccc gtccggtgat cggttcaaag ttgtgaatac tgaaggcata tggatataca
      781 taaccgtccc acccgatcag atcgaacgga tgatgcatat aatgatacgg atacatgatg
      841 ccctgcttct tgatgtagaa gaggaagtcg ccggtctcat catgcgtttg caggttgaca
      901 ggcagcttga tgtcacgctc acagaatggc gaatgttcca gcaactgccc caccttgttc
      961 atatacctct tcgggaagtc gatcggagaa aaactctcca ctataagcag gcggttgttc
     1021 tcatcgttga aatggagctg ataaacagtt cctctgggta tgatgacata atcgccgtat
     1081 tcaaagctca cttcaccata agcactcagc agtttaccgc tgccttcatg tatgaagatc
     1141 atctcatcgg catcggtgtt cttgtagaaa taatctgtca tgctcttctt cggagctgca
     1201 agagatatgt ggaggtcgtt gttgaccagt acggcttttc tgcttttcag gtaatcatct
     1261 tccggctgaa tgttgaaccc cttcaggctt ctgggcttca gctgtttgct cagcaccact
     1321 t
//
LOCUS       DAOMAX010000492         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_31110_length_1299_cov_5.170418, whole genome shotgun sequence.
ACCESSION   DAOMAX010000492 DAOMAX010000000
VERSION     DAOMAX010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31110_length_1299_cov_5.170418"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1228
                     /locus_tag="PK742_14210"
     CDS             join(69..321,321..1228)
                     /locus_tag="PK742_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611658.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HQU77796.1"
                     /translation="MLKRKQYTKEFKLDAISLVMDQGLTIAEAARSLEINANMLNRWI
                     KESQADNNGQAFRGNGKLTPEQEEIRNLKLQIKQLKLEKQNIKGGGGLLRERNEVKYS
                     FITQKKKTCPVGTMCRLLGVSRSAYYGHEQCRRNRPDDLYHRQLLDAVLDIARSCDYT
                     YGTRRMKRALNASGYPVSRWKARKLMREAVVAVRHRKKYKVTTDSNHPLPVFENQLNR
                     QFTVTGPDQAYVCDITYIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKASLVCDALRM
                     AIWLRRPRPGLIVHSDRGSQYASKAYRNLLKAHGFIGSMSRLGNCWDNAVAESFFGSL
                     KRERCQWRHYQTRYEAQQDILQYIAVFYNSQRLHSYLDYKSPNQYEAEAVKG"
BASE COUNT          381 a          255 c          351 g          312 t
ORIGIN      
        1 cataatgaca tggtctaata gacccggaca ctccagttaa gagaaaatag acttaattgg
       61 aggaagagat gttgaagaga aaacaataca caaaagaatt caaactggat gcgattagtc
      121 tggttatgga tcaggggttg acgatcgcag aagcggccag gagccttgaa attaatgcca
      181 atatgctcaa tcggtggatt aaggaaagcc aggcagataa caatggtcag gcatttcgcg
      241 gcaatggaaa gttgacgccg gaacaggaag aaattaggaa tcttaaatta caaatcaaac
      301 aactcaagct ggagaaacag atattaaagg aggcggcggt cttcttcgcg agagaaacga
      361 agtgaaatat tcgttcatca cccaaaagaa gaagacctgt ccggtcggca caatgtgtcg
      421 gctattgggt gtcagccgca gtgcttatta tggccatgag caatgccgta gaaatcgccc
      481 cgatgatttg tatcacaggc aactgcttga tgcagtgctg gatattgcaa gatcgtgcga
      541 ttacacctat ggcactcgca ggatgaaacg ggcgctgaat gcgtcaggtt atccggttag
      601 tcgctggaag gcgagaaaac tcatgcgtga agctgtagtg gctgtacggc atcggaagaa
      661 atacaaggta acgacggaca gcaatcaccc gttaccggtg tttgagaacc aattgaatcg
      721 gcaatttact gtgaccgggc cggatcaggc ttatgtttgc gatatcacgt acatttggac
      781 ccaggaaggc tggttgtatc tggcggtagt cattgatttg ttttccagaa aagtggtcgg
      841 ttggagtatg agttcgcgga tgaaagcaag cctggtgtgc gatgcattgc gaatggctat
      901 ctggctgcgc cgacccaggc caggattgat agtacattca gatcgcggat cgcagtatgc
      961 cagcaaagct taccgcaatc tactgaaagc acacggtttt attggcagca tgagccgttt
     1021 gggaaattgc tgggataatg cggttgctga gagctttttt ggtagcctca aacgggaacg
     1081 ttgccagtgg cgacactacc agacccggta tgaagcacag caggatatat tgcagtatat
     1141 cgccgtgttt tataacagcc agagactgca ttcatacctg gattacaaaa gccccaatca
     1201 atatgaagca gaagcagtaa aaggatgaat gttgcttaac tgatgtgtcc ggttttactt
     1261 gaccaggtca tcattatcag gcagtttatt ttattgatt
//
LOCUS       DAOMAX010000493         1283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_31856_length_1283_cov_3.507329, whole genome shotgun sequence.
ACCESSION   DAOMAX010000493 DAOMAX010000000
VERSION     DAOMAX010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31856_length_1283_cov_3.507329"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1283
                     /locus_tag="PK742_14215"
     CDS             <1..>1283
                     /locus_tag="PK742_14215"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HQU77797.1"
                     /translation="DLCAKHGIQFTILAPRQIEAVRLHKVDSWTEVHEDSVDTGRPYL
                     VETPSGRTITVFVYHGELSRGVAFNGLLNSGRMFADSILAAFPKDQETHLVNLATDGE
                     SYGHHHRNGDMALASCLDFLDLEEKVEVINYATFLEKYPATAYARIRENSSWSCVHGI
                     ERWRSNCGCQDGAHADYHQKWRKPLRDALDWLKALADTLYEKEVGTYCPDPWMLRNKY
                     IRVILNRDQQEADTMLDDCLPEEVDEKTREFIIRLLEMQRNAMLMFTSCAWFFDDVSR
                     IETLQVLQYADRVIQIAEGETKKEILHDFLKRLSQAPVNAPGWEDAAELYVSKIRSKR
                     LDLNSVAMHYAAASLFEHKPEKLQLFNYTYENIFFERFESASHKLSVGHIRVRSRVTF
                     YERPFYFAALHLGQQHIVGHFANAMDVDAFETMHL"
BASE COUNT          335 a          325 c          338 g          285 t
ORIGIN      
        1 tggacctctg tgccaaacat ggcattcaat ttaccatcct cgctccgcga cagatcgaag
       61 ctgtcagact gcacaaagtt gacagctgga cagaagtaca tgaagattca gtggatacag
      121 gccgaccata tctggtggaa acgccatcgg gaagaaccat cacggtcttt gtgtatcatg
      181 gagaactgag cagaggtgtg gctttcaacg gactgctcaa ttcgggacgg atgttcgctg
      241 acagtatcct ggccgcgttc ccgaaagatc aggagaccca cctggtcaat ctggccaccg
      301 atggagaaag ctacggccac caccatcgca acggcgatat ggccctggcc tcctgcctgg
      361 attttctgga tctggaggaa aaagtggaag tgatcaacta tgccactttc ctggagaaat
      421 atcccgctac ggcgtatgcc cgcatccgtg agaactccag ctggagctgt gtacacggca
      481 tcgaacgctg gcgaagtaat tgcggctgtc aggacggggc acatgcagac taccaccaga
      541 aatggcgcaa gccgctgcgt gatgcacttg actggctgaa agcactggca gataccctct
      601 atgaaaaaga agtgggcacc tactgcccgg atccctggat gctgcgcaat aaatacatcc
      661 gcgtcattct gaacagagac cagcaggaag cagataccat gctggacgat tgtttacccg
      721 aagaggtgga cgaaaagacc cgcgagttca ttatccggtt actggagatg cagcgcaatg
      781 ccatgctcat gtttaccagc tgtgcctggt tcttcgatga tgtatcacgc attgagacct
      841 tacaggtttt gcagtatgct gacagggtca tacagatagc cgaaggagag accaaaaaag
      901 agatcctgca cgattttctg aagcgactca gccaggctcc tgtaaatgct ccgggatggg
      961 aagatgctgc tgaactctat gtcagcaaga tcagaagcaa gcgcctggac ctcaacagtg
     1021 tggccatgca ctatgctgct gccagcctgt tcgaacataa gccggagaaa ctgcagctct
     1081 ttaactatac ctacgagaat attttttttg aacgatttga atcagcttct cacaaattaa
     1141 gtgtggggca catcagggtg cgcagccggg tgaccttcta tgaacgtcct ttctattttg
     1201 cagcactgca tctgggtcag cagcatatag taggacactt tgccaatgcc atggacgtgg
     1261 acgcttttga gaccatgcac ctg
//
LOCUS       DAOMAX010000494         1257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_33056_length_1257_cov_3.495840, whole genome shotgun sequence.
ACCESSION   DAOMAX010000494 DAOMAX010000000
VERSION     DAOMAX010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33056_length_1257_cov_3.495840"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1257)
                     /locus_tag="PK742_14220"
     CDS             complement(5..>1257)
                     /locus_tag="PK742_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQU77798.1"
                     /translation="IYTGKERPKYHGELPEGNDGLGLMLLGVTGDQVLPKETYEEIKA
                     WTLTQVRGTVQADILKEDQAQNTCIFSTEFALRLMGDVQAYFIDHKVRNFYSVSISGY
                     HIAEAGANPITQLAFTLANGFTYVEYYLSRGMDINKFGPNLSFFFSNGIDPEYAVIGR
                     VARRIWAKAMKQKYGADERTQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNC
                     NSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPLQGSFIIEELTDLVEE
                     AVLSEFDRITERGGVLGAMETMYQRSKIQEESLYYETLKHTGEYPIIGVNTFLSSKGS
                     PTILPKEVIRSTEEEKMAQIHTVEQLQQRHAEKGEKLLREVQLSAIHNENIFAQLMEA
                     GKYCSLGQITTALFEVGGQYRRNM"
BASE COUNT          242 a          354 c          322 g          339 t
ORIGIN      
        1 tccatcacat attcctcctg tactgtccgc ctacttcgaa cagggcggtg gtgatctgtc
       61 ccaacgaaca atacttgccc gcttccatca gctgtgcaaa gatgttctca ttgtggatgg
      121 ccgacaattg cacttcccga agcagttttt cgcctttctc tgcgtgacgc tgctgcagct
      181 gttccaccgt gtggatctgt gccatcttct cttcttcggt agaacggatc acttccttcg
      241 gcaggatggt gggagaaccc ttggaactca ggaaggtgtt caccccgatg atgggatact
      301 cgcccgtatg tttcagggtc tcgtaataca gactttcttc ctggatcttg ctgcgctggt
      361 acatggtctc catggctccc agcacaccac cccgttcggt gatgcggtcg aactctgaca
      421 acacggcctc ttccaccaga tcggtcagct cttcgatgat gaaactgccc tgcagcggat
      481 tctcattctt cgccagaccg agctccttgt tgatgatgag ctgtatagcc atggcacgac
      541 gcacactttc ttctgtcggc gtggtgatgg cctcgtcgta agcattcgtg tgcaaactat
      601 tgcagttgtc atagatggca tacagtgcct gcaacgtggt gcggatgtcg ttgaagtcga
      661 tctcttgtgc atgcagactt ctgcccgatg tctgaatatg gtatttcagt ttctgcgtgc
      721 gttcgtcggc accgtacttc tgcttcatgg ccttggccca gatgcgtctg gcaacccgtc
      781 cgatgaccgc atattccggg tcgatgccat tgctgaagaa gaaggacaaa ttcggaccga
      841 acttgttgat gtccatcccg cggctcagat aatattccac atacgtgaaa ccattcgcca
      901 gggtgaaggc cagctgggtg atgggattgg cacccgcttc ggcaatgtgg taaccgctga
      961 tagatacgga atagaaattc cgcaccttgt ggtcaatgaa ataagcctgt acatcgccca
     1021 tgagcctgag cgcaaactcg gtactgaaga tgcaggtgtt ctgtgcctgg tcttctttca
     1081 ggatatcggc ctgcaccgtt ccacgcacct gtgtcagggt ccatgccttg atctcttcgt
     1141 aggtctcctt cggcagcacc tgatcgccgg tcacacccag cagcatcaga ccgagtccgt
     1201 cgttgccttc gggcagctcg ccgtggtatt tcggtctttc cttcccggta tagatcg
//
LOCUS       DAOMAX010000495         1251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_33363_length_1251_cov_2.824415, whole genome shotgun sequence.
ACCESSION   DAOMAX010000495 DAOMAX010000000
VERSION     DAOMAX010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33363_length_1251_cov_2.824415"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1251)
                     /locus_tag="PK742_14225"
     CDS             complement(<1..>1251)
                     /locus_tag="PK742_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQU77799.1"
                     /translation="QVKSIPVLFGETDILKTLQLLPGVGGAGEGNSGFYVRGGGPDQN
                     LILLDEAVVYNTGHLFGFFSVFNGDAIKNTTLVKGGMPAQYGGRLSSVVDVQMKDGNN
                     QQFHAQGGIGLIASRLTLEGPIVKEKSSFMVSARRTYIDALITPFIKGTDFEGNAYYF
                     YDVNAKANYRFSDKDRLFLSGYFGRDVFNYRSPDDDFNVEVPWGNATGTLRWNHLFSD
                     RMFMNMSAIFNDYNFEVSSAFDQFDFKLYSGVRDWNFKTDFDYYLNANHTFKYGVNYI
                     YHAFTPYSAQGSSGGVEFGTDSLNKKYAHESAIYFQDEFNVGALWRFNVGVRGSYFVH
                     TGPLNELVYDEVTGLPIDTIYRSAGDAIADYWGLEPRINARYIIDQQSSLKAGIAVTN
                     QFIHLVANSVSTLPTDLWVPSSAV"
BASE COUNT          292 a          309 c          350 g          300 t
ORIGIN      
        1 cgactgccga gctgggcacc cagagatcgg tgggaagggt gctgacggaa ttggcaacca
       61 ggtggatgaa ctgattggtg accgcgatac ctgccttgag cgacgattgc tggtcgatga
      121 tgtaacgtgc attgatgcgt ggctccaaac cccagtagtc ggcgatggca tctcctgccg
      181 aacgatagat ggtatcgatg ggcagaccgg tcacttcatc atagaccagt tcgttgagcg
      241 gaccggtatg tacgaaatag gatccgcgaa cacctacatt gaaacgccag agtgctccca
      301 cgttgaattc gtcctggaaa tagatggcag attcatgcgc atacttcttg ttcagcgaat
      361 ccgtgccgaa ttccaccccg ccactagaac cttgtgcact atacggtgtg aaggcgtggt
      421 agatataatt cacgccgtac ttgaaggtgt ggttggcgtt gaggtagtag tcgaaatcgg
      481 tcttgaagtt ccagtcgcgg acaccactgt acagtttgaa atcgaactga tcgaatgcgc
      541 tgctcacttc aaagttgtag tcgttgaaaa tggccgacat gttcatgaac attcggtcgc
      601 taaacaggtg attccagcgg agggtaccgg tggcatttcc ccagggcact tccacgttga
      661 aatcgtcgtc gggtgaacgg tagttgaata catcccggcc gaaatatccg ctcaggaaca
      721 ggcggtcctt gtcgctgaag cgatagttcg ccttggcatt cacatcgtag aaataatagg
      781 cgttcccttc aaagtcggtg cctttgatga agggtgtgat gagtgcatcg atataggttc
      841 ttctggccga gaccatgaag gaagacttct ctttcacgat gggaccttcc agggtaagac
      901 gcgatgcgat gagtccgata ccgccctgtg catggaactg ctggttgttg ccatctttca
      961 tctgcacatc gaccaccgag ctgagtcggc cgccgtattg tgccggcata ccgcctttca
     1021 cgagtgtggt gttcttgatg gcatcaccgt tgaagacgga gaagaaaccg aagaggtgac
     1081 cggtgttata gaccaccgct tcgtctaata agatgaggtt ctgatccggt ccgccgcctc
     1141 tcacataaaa accggagttg ccctcacctg ctccgcccac cccgggaagg agctgcaggg
     1201 tcttgaggat atctgtttcg ccgaaaagaa caggaattga tttcacctgt t
//
LOCUS       DAOMAX010000496         1250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_33409_length_1250_cov_3.434310, whole genome shotgun sequence.
ACCESSION   DAOMAX010000496 DAOMAX010000000
VERSION     DAOMAX010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33409_length_1250_cov_3.434310"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1002
                     /locus_tag="PK742_14230"
     CDS             250..1002
                     /locus_tag="PK742_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77800.1"
                     /translation="MRRSICATLLMAALPAGLLAQTNVQDSCVQANLVLVEAGIGLPQ
                     ADLADRFGYHGLAGAGWQFKSDKNLLFGLSGHYYFGDRVKEDSLLFNLRDDNGYIIGN
                     DGALFIPSIFEQGFDVMFHFGKITSLLSHNPNSGLTVMGGVGFFEHNIQIYIDEAYVP
                     QLNDTYKKGYDRLTNGIMFSEYIGYSYFSGKHFVNFRAGLEWSQAITKGRRGITFDTL
                     EPQDATRLDMMINLKLTWVLPVYKQTGRSFYY"
     gene            1002..>1250
                     /locus_tag="PK742_14235"
     CDS             1002..>1250
                     /locus_tag="PK742_14235"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQU77801.1"
                     /translation="MRVSWSKRLYRLGTGAYQLGIRSATPFSSKARAWTEGRQATASA
                     PESFQPGEAVVWFHCASVGEFEQGRPLIELLKSRYQQIQ"
BASE COUNT          319 a          289 c          305 g          337 t
ORIGIN      
        1 agggcaaaca atgagaaact ccaagatact cttcaagacc ccgggtcaga ttcctgacca
       61 tcggaccgtt aatgaaggca gtagtttcta ttacttctct gataccgctg tcaatttcgt
      121 tccggatctg tttatactgg ccagccagat cgaccatctg aatcttctgc atgcaggatt
      181 tttacggacg caaagataat caaggccaca gaagtccgtt ccatttgtgc tgttgcttac
      241 ctttgacgca tgaggagatc gatctgtgca actctgttga tggctgcctt acctgccggt
      301 ctgctggctc agaccaatgt acaggatagc tgtgtccagg caaacctggt actggttgaa
      361 gccggtattg gtttaccaca ggctgatctg gctgaccggt tcggatatca tggacttgcc
      421 ggtgcgggat ggcagttcaa atcagataag aacctgctgt ttggcctgtc cggtcattat
      481 tactttggcg accgggtaaa agaagacagc ctgctgttca atctgcgtga tgacaatggc
      541 tatatcattg gcaatgacgg tgctttgttc attccttcca tctttgaaca gggttttgac
      601 gtgatgtttc atttcggcaa gatcacttca ctgctatcgc acaaccccaa ttcaggctta
      661 acggtcatgg gaggtgtggg gttctttgaa cacaatattc agatctatat agatgaagca
      721 tatgtgccac agctgaatga cacatataag aaaggatatg accgacttac caatggaatc
      781 atgttcagtg aatacattgg gtattcgtac ttcagcggaa aacattttgt gaatttcaga
      841 gccggcctcg aatggtcgca agctatcacc aaaggcagaa ggggcatcac ctttgatact
      901 ctggaaccgc aggatgccac cagactggat atgatgatca atcttaaact cacctgggtg
      961 cttccggtct ataagcaaac cggtagaagc ttttattatt gatgagagta tcctggagca
     1021 aacgattgta tcgcctcggt acaggtgctt accagcttgg tatcagatca gcaactcctt
     1081 tttcttccaa agcacgggca tggaccgaag gccgccaggc caccgcttct gcacctgaaa
     1141 gttttcaacc gggagaagcg gtggtatggt ttcattgcgc ttctgttggc gaatttgaac
     1201 aaggaaggcc cctcatagag ctcctgaaat cgaggtacca gcaaattcag
//
LOCUS       DAOMAX010000497         1245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_33679_length_1245_cov_3.178992, whole genome shotgun sequence.
ACCESSION   DAOMAX010000497 DAOMAX010000000
VERSION     DAOMAX010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33679_length_1245_cov_3.178992"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK742_14240"
     CDS             <1..399
                     /locus_tag="PK742_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQU77802.1"
                     /translation="GIVVITGGEPLLYDLTELTRGIREKGLPVHLETSGTHPLTGTWD
                     WICWSPKKFRNSLTGIPSAAHELKVVVYNQSDFAWAELHRAQVTDKCRLFLQPEWSKS
                     TVMLPMLISYVQENPDWHISLQTHKYMQIP"
     gene            complement(449..>1245)
                     /locus_tag="PK742_14245"
     CDS             complement(449..>1245)
                     /locus_tag="PK742_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77803.1"
                     /translation="PIFAGPFTPVGTAWTGSSFGHARIEGSDLNNLIVDQAAPSNVVL
                     AEKYYGAGVVLFGGMTTNNFHTPATEAANLRANIISYLSCAGGDVCEAVTGLSGDPGT
                     TTATLSWDDMGATKYRFAFGKVGDPGTFQAGQVFTTFVTFSGLTPGATYTARVARDCY
                     PDGWSATSEITWTQSLRLGQVEQEVLLYPNPSNGQFRLQLNGYENSDLNIMIANTMGQ
                     VVYNTNINVNSAIMVQDINLSDAAAGTYIVRLTNGTETSVQTLVIE"
BASE COUNT          347 a          289 c          307 g          302 t
ORIGIN      
        1 ggcatagtgg tcattaccgg cggtgaaccg ctcctctatg acctgaccga attaacaaga
       61 ggtatcagag aaaaaggatt acctgttcac ctggaaacgt caggaacaca cccgttgacc
      121 ggaacctggg actggatctg ctggtcgccg aaaaaattca ggaattcact tacgggcatt
      181 ccatctgcag ctcatgaatt aaaagtggta gtatataacc agtcagattt tgcatgggca
      241 gaattgcatc gagcccaggt tacagataag tgccggctgt ttttgcagcc tgaatggagc
      301 aagagtacag tcatgctgcc tatgctgatc agctatgttc aggagaatcc cgactggcat
      361 atctcattgc agacgcataa gtatatgcag ataccataat aaaaaaggga agcataaagc
      421 ttcccttttt tattacttat gtctggtatt actcaataac aagggtctga acgctggttt
      481 cggtaccatt ggtcagacgt acaatgtatg taccggcagc tgcatcagac aggttgatat
      541 cctgtaccat gatagcactg ttcacattga tattggtgtt atataccact tgtcccatgg
      601 tattggcgat catgatattc agatctgaat tttcataacc gttcaactgc agacggaact
      661 gtccgttgct ggggtttgga tagagcaaca cttcctgctc cacctgaccc aaacgaagtg
      721 actgtgtcca ggtgatctct gaagtagcag accatccgtc aggataacag tcgcgggcaa
      781 cacgagcagt ataggtagca ccaggtgtaa gacctgagaa ggtcacgaaa gtggtgaata
      841 cctgtcctgc ctggaaggta cccggatcac ctaccttgcc aaatgcaaag cggtatttgg
      901 tagcacccat atcatcccag ctcagggtag cagtagttgt accgggatca ccggagaggc
      961 ctgttacggc ttcacataca tctccgcctg cacaagacag gtaagaaatg atgttggcac
     1021 gcaggttggc cgcttcagtt gcaggggtgt ggaagttatt ggtggtcata ccaccaaaca
     1081 gcacaacacc tgcaccgtag tatttctcag cgagaaccac attggaagga gctgcctgat
     1141 caacgataag gttattcaga tctgaacctt caatccgggc atgtccaaaa gaagaacctg
     1201 tccaggctgt tccaaccgga gtaaatggac ctgcgaaaat cgggt
//
LOCUS       DAOMAX010000498         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_34228_length_1235_cov_3.680508, whole genome shotgun sequence.
ACCESSION   DAOMAX010000498 DAOMAX010000000
VERSION     DAOMAX010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34228_length_1235_cov_3.680508"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK742_14250"
     CDS             complement(<1..182)
                     /locus_tag="PK742_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HQU77804.1"
                     /translation="MQTVITGTGSYIPTEVKTNRDFTIHDFYAENHTRITTPPEEVTS
                     KFQQITGIEERRYAVP"
     gene            complement(187..>1235)
                     /locus_tag="PK742_14255"
     CDS             complement(187..>1235)
                     /locus_tag="PK742_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQU77805.1"
                     /translation="HFCTADIGWITGHSYIIYGPLAAGATTLMFEGIPTWPDAGRFWD
                     IVDKYRVNMLYTAPTAIRSLMSFGLGPLQDHKLDSLKVLGTVGEPINEEAWHWYHDHV
                     GKRKCPVVDTWWQTETGGIMISNMAGITPEKPSYATLPLPGVQPVLVDENGQEITGNE
                     VSGNLCIRFPWPGMIRTTYGDHERCRQNYFATYDDLYFTGDGCYRDENGMYRITGRVD
                     DVLNVSGHRIGTAEVENAINMHNGVVESAVVGYPHDIKGQGIYAYVIFSHEISEADLT
                     RRDIAHTVSRIIGPIAKPDKIQFVPGLPKTRSGKIMRRILRKIAEGETTSLGDTSTLL
                     DPGIVQHILEGKIH"
BASE COUNT          280 a          307 c          305 g          343 t
ORIGIN      
        1 tcaggtacag cataccgacg ctcttcaatg ccggtgatct gctggaattt gctggtcacc
       61 tcttcgggcg gggtggtgat gcgggtatga ttttctgcgt agaaatcatg aatggtaaag
      121 tctctgttgg tcttgacctc agtaggtata taggaaccgg ttccggtaat gactgtctgc
      181 atgttgttag tgtatttttc cttccagaat atgttgtact atgccaggat ccagcagggt
      241 ggaagtatcg cccaggctgg ttgtttctcc ttcagctatt ttgcgcagta ttcttctcat
      301 gatcttgccc gaacgggttt tgggaagccc gggaacgaac tggatcttgt caggtttagc
      361 tatcgggccg atgatcctgg aaactgtatg cgcaatatca cgccgtgtca ggtcggcctc
      421 actgatctca tgactgaaga tcacataggc ataaataccc tggcctttga tatcatgagg
      481 atagcctact actgcactct ctaccacacc gttgtgcata ttgatggcat tttccacttc
      541 ggcggtacct atgcggtgac cacttacatt cagcacatcg tccacccgtc cggttatccg
      601 atacatgcca ttttcatcgc ggtaacaacc atcacccgta aagtaaaggt catcgtaggt
      661 agcaaagtaa ttctggcggc agcgctcatg atcaccataa gtggttctga tcatccccgg
      721 ccagggaaaa cgaatgcaaa gatttccgct cacttcatta cctgttattt cctgtccgtt
      781 ctcatctacc agaacaggtt gaacaccggg taaaggcaag gttgcgtaag atggcttttc
      841 aggtgtgatg cctgccatgt tggaaatcat gataccaccg gtttctgttt gccaccaggt
      901 atctaccaca gggcatttcc gtttgcccac atggtcatga taccagtgcc atgcctcttc
      961 attgatgggc tcgccaaccg ttccgagtac ctttaggctg tccagtttat ggtcttgcaa
     1021 gggtccgagg ccaaagctca tcagacttct gatggcagta ggtgctgtat agagcatgtt
     1081 gacccgatat ttgtcaacta tatcccagaa tcttccggca tccggccagg taggaatgcc
     1141 ctcgaacatc agcgtggtag caccagcagc cagagggccg tatatgatat aggaatgtcc
     1201 ggtgatccag ccgatatctg ccgtgcagaa gtgta
//
LOCUS       DAOMAX010000499         1234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_34273_length_1234_cov_3.849873, whole genome shotgun sequence.
ACCESSION   DAOMAX010000499 DAOMAX010000000
VERSION     DAOMAX010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34273_length_1234_cov_3.849873"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>1234
                     /locus_tag="PK742_14260"
     CDS             105..>1234
                     /locus_tag="PK742_14260"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HQU77806.1"
                     /translation="MLRSSVIFFILLTTVGRTQTLPFTWLYDDEQVYSMYINLPADSL
                     EDMFDNLENEYYYHATFIYTDGITADTVEDIGLRLRGNTSLYSAKKSYKVSFNTYSPG
                     RKYEGVEKLNLLGMHNDPTMVREHLYFDTYIELGLPEHRSNFVRLYLNGEYFGLYTNM
                     EHIDEVYVNDRFGNNDGNLYKCTWPADLNWRGSDQNDYLDNGYELHINEDNPDASDLI
                     HFIDVLNNTPIADLPCELEKVFNVQEFLKTYALDVAAGHWDNYGANINNYYLYHNLAT
                     DKFEFLSFDCDNTFGVDWLGFDWTNQPIYDWPTGWYDVPLVTRLMEVDTYRKQFGYYL
                     EQIHQTALDPVYAGSKVLAWRDLVAAAALEDDYRTYDWGYTY"
BASE COUNT          327 a          323 c          295 g          289 t
ORIGIN      
        1 taccagcact tcctgcgctt tcaggccgga acatcccgga gtgaacaaga cgaagaacag
       61 ggcaatagcc ttgccagccg tatatttgta tgatgcgtgc atgtatgctc cgaagttcgg
      121 taatcttttt catcctgctg accaccgtcg gccgcacgca aaccttaccc ttcacctggc
      181 tgtatgacga tgagcaggtg tactccatgt atatcaacct gcctgcagat tcgctggaag
      241 acatgttcga taacctggag aatgaatact attaccacgc cacattcatt tatacggacg
      301 gcatcaccgc agatacggta gaagatatcg gactgcgcct gcggggaaac acctctctct
      361 attctgccaa gaaatcatac aaggtctcct tcaataccta cagtcctggc agaaaatatg
      421 aaggggtaga aaagctcaac ctgctgggca tgcacaacga tcccaccatg gtgcgggagc
      481 acttgtattt cgatacctat attgaactgg gactgccgga acaccgcagc aattttgtgc
      541 gtctctacct caacggtgaa tacttcggac tctacactaa tatggaacat attgatgagg
      601 tgtatgtgaa cgaccgattt ggtaacaacg atgggaatct ttacaaatgc acctggcctg
      661 ccgatctcaa ctggcgtgga agcgatcaga atgactacct ggacaacggc tatgaactgc
      721 atatcaacga agacaacccg gatgccagcg acctcatcca cttcattgat gtactcaaca
      781 acacgccgat tgcggatctg ccctgtgagt tggagaaagt gttcaacgta caggaattcc
      841 tgaaaaccta tgcgctggat gttgctgcag gtcactggga taactacgga gccaacatca
      901 acaactatta tctctaccac aatctcgcca ctgacaagtt cgagtttctt tcgttcgact
      961 gcgacaacac ctttggggtg gactggctcg gtttcgactg gaccaatcag cctatctacg
     1021 actggcccac cggctggtat gatgtaccac tggttacccg gttgatggaa gtggatacct
     1081 atcgcaagca gttcggatat tacctggaac agatccatca gaccgcactg gatccggtct
     1141 atgcgggaag caaagtattg gcatggcgtg acctggtagc agcagctgcg ctggaagacg
     1201 actaccgcac ctacgactgg ggctatacct atga
//
LOCUS       DAOMAX010000500         1226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_34689_length_1226_cov_3.176772, whole genome shotgun sequence.
ACCESSION   DAOMAX010000500 DAOMAX010000000
VERSION     DAOMAX010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34689_length_1226_cov_3.176772"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1077)
                     /locus_tag="PK742_14265"
     CDS             complement(244..1077)
                     /locus_tag="PK742_14265"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQU77807.1"
                     /translation="MKRVVITCLLAWVPVLLSAQITGYEVGDIAPNFTGIEPDGTTHK
                     LYDYTASGQYVLLDFFAYWCGPCYTNAGYLQAFYEKYGCNNGTVAVLGNECDVGGSND
                     DLDLFFELSGLDPATAYPSWSGMDGNGADVCNIYSPAAYPTVVLIGPDNRFISIDIWP
                     IASLGDIEDAFPNGVLMPIDCNWTSSVSDRPEPLFGLSLWPNPASEKITISMVTPAAG
                     AMEWSLVSLTGVPVQEGLLEQVSAGRQQQTICLQDLPSGLYVFHYSMNGQDGSSLLQI
                     Q"
BASE COUNT          329 a          330 c          288 g          279 t
ORIGIN      
        1 aaacgaacat gcatttgtta tacgggagcc tccttcgtcg gcttcaggaa tgacgtgtcc
       61 ttttgagggt attgatcggt tattggaaac acatgccagc aaacgatccc gacagagcta
      121 tcagaattca cctatcactc ctcacctctt accattaact cacagcacct ccgtattccg
      181 ccctccgcat taaaaaaggg gaatgtttcc atcccccatg actatgaaaa cacctaatca
      241 tatttattgg atctgcagga ggctgctgcc atcctgacca ttcatgctat aatggaatac
      301 atacaaccct gatggaaggt cctgcagaca gatggtttgc tgctgcctgc ccgccgaaac
      361 ctgctccagg aggccttcct gtaccggtac accggtcagg cttacgagcg accattccat
      421 tgccccggca gcaggtgtca ccatactgat ggtaattttt tccgatgcgg gattaggcca
      481 aagagacagt ccgaataaag gttcgggtct gtcagaaaca gacgatgtcc aattgcaatc
      541 aatcggcatg agtacaccat tggggaatgc atcttctata tcacccaggc tggcaatcgg
      601 ccagatgtcg atgctgatga atctgttatc aggacctatc agcaccaccg taggataggc
      661 cgcaggcgag tagatattgc atacatcggc accatttccg tccatcccgc tccaggaagg
      721 ataggccgtt gcaggatcca gtccggacag ttcaaagaag aggtccaggt catcgttgct
      781 tccccccaca tcacattcat ttcccagcac tgctacggta ccgttgttgc aaccatactt
      841 ctcataaaat gcctgcaggt agccggcatt cgtatagcag ggcccacacc agtaggcaaa
      901 gaaatccagc aacacatact gtccgctggc ggtatagtca tacaatttat gggtggtacc
      961 atcgggttcg atacctgtaa aattgggagc aatatcacct acttcatacc cggttatctg
     1021 ggcagatagc agcaccggca cccaagccag caggcaggtg atcaccactc ttttcacagt
     1081 ataaaagtac ggatgataag ccggcagaac acatgctttg ctctaaatgt cataaagatg
     1141 acacagctat tcacagccag gatagaacgg gttggcggtg gaggaaggaa agcccaggcc
     1201 aaaggtccgg tatccgcagg ccaggg
//
LOCUS       DAOMAX010000501         1129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_40615_length_1129_cov_3.184358, whole genome shotgun sequence.
ACCESSION   DAOMAX010000501 DAOMAX010000000
VERSION     DAOMAX010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40615_length_1129_cov_3.184358"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1129
                     /locus_tag="PK742_14270"
     CDS             <1..>1129
                     /locus_tag="PK742_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQU77808.1"
                     /translation="RKLAAKSTAPILGITGTGGAGKSSLVDEMVRRYLMQFPDKTIGI
                     ISVDPSKRKTGGALLGDRIRMNSINNPRVYMRSLATRQANLALSQYVGDAVSVLKAAG
                     YDLIILETSGIGQSDTEIIDHSDVSLYVMTPEYGAASQLEKIDMLDFADIIALNKFDK
                     RGAMDALRDVRKQYQRNHKLFDKQVDSMPVFGTIASQFNDPGANRLFQELMDTIVRKT
                     GNEAFKIHYEADEEMSEKVYIIPPNRTRYLSEISENNRNYDVWVQAQSDTADQLYAIQ
                     TAIDSLEKAGVSKDDVSLQGLRTHYDAVALSFDNNNKKILDEWADKRASYKKDIFEFT
                     VRDKTLKVKTHTQSLSHLHIPKVVTPRYRSWGDILTWSLQEN"
BASE COUNT          316 a          303 c          289 g          221 t
ORIGIN      
        1 gcgcaaactg gctgctaaaa gcaccgctcc catcctgggt atcaccggaa caggcggagc
       61 tggaaaatca agtctggtag atgagatggt tcgccgttat ctgatgcagt ttcccgacaa
      121 gaccatcggc atcatttcgg tggatccctc caaaagaaaa accggaggag cattactggg
      181 cgaccgtatc cgcatgaaca gcatcaacaa cccaagggtc tatatgcgtt cgctggctac
      241 ccgtcaggcc aacctggccc tgagtcagta tgtgggtgat gcagtttctg tgctgaaagc
      301 cgccggttac gatctcatca tcctggaaac atccggcatc ggacagagcg atacagagat
      361 cattgaccac agcgatgtga gcctgtatgt gatgaccccg gaatacggag cagcatcgca
      421 gctggagaag atcgatatgc tggacttcgc agacatcatc gccctgaata aattcgacaa
      481 gcggggtgct atggatgccc tgcgtgatgt gcgcaagcaa taccagcgca accataaatt
      541 atttgacaag caggtggaca gcatgccggt gttcggcacc atcgccagtc agttcaacga
      601 ccccggtgcc aacagactgt tccaggaact gatggatacc atcgttcgca agaccggcaa
      661 cgaggctttt aagatccact acgaagcgga tgaggaaatg agcgagaagg tctatatcat
      721 cccgcccaac cgcacccgct atctcagtga gatatcagag aacaaccgga attacgatgt
      781 ctgggtacag gcacaaagtg acacagccga tcagttgtat gccattcaaa cagccatcga
      841 cagcctggag aaagccggcg tgagcaaaga cgacgttagt ctgcagggtc ttcgcacgca
      901 ctatgatgcg gtagctctct ccttcgacaa caacaataag aagatcctgg acgaatgggc
      961 agacaaacga gcctcctata aaaaggacat ttttgaattc accgtccgcg acaaaaccct
     1021 gaaggtcaag acgcatacac agtcgctcag tcaccttcac attcccaagg tggtaacacc
     1081 acgttaccgc agctggggcg atattctcac ctggagtctg caggaaaac
//
LOCUS       DAOMAX010000502         1118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_41407_length_1118_cov_3.518344, whole genome shotgun sequence.
ACCESSION   DAOMAX010000502 DAOMAX010000000
VERSION     DAOMAX010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41407_length_1118_cov_3.518344"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PK742_14275"
     CDS             complement(<1..520)
                     /locus_tag="PK742_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQU77809.1"
                     /translation="MKGIILAGGSGTRLHPITLAISKQLMPIYDKPMIYYPLSTLMMA
                     GINEILIISTPYDLPGFQKLLGDGSRLGCSFSYVEQPSPDGLAQAFTLGADFIGKDKV
                     ALVLGDNIFYGVGLGDLLANNNDPDGGIIYAYHVSDPERYGVVEFDADKKVISIEEKP
                     AAPKSNFAVPGLY"
     gene            complement(563..>1118)
                     /locus_tag="PK742_14280"
     CDS             complement(563..>1118)
                     /locus_tag="PK742_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQU77810.1"
                     /translation="LLALLVFLYLIWLALSMRKKPVPTDHPVAAKHIRWVLGIIVVQI
                     AFGGFMAGTKAGLLFQTWPLMNGTVIPPNAFHGANAMAAIFDNPTTINFVHRTLGILV
                     FLAVGIFWLQNRRFSAPSMRRLEHALLLMVFLQFLLGVTTLLLASTHIPVFWGVVHQL
                     GAFILAGIATAILYYARPRKPLIG"
BASE COUNT          287 a          284 c          316 g          231 t
ORIGIN      
        1 aatagaggcc gggaaccgca aagttggatt taggagctgc aggtttttcc tcaatagaaa
       61 tgaccttttt atcggcatcg aattccacca caccatagcg ttccggatca ctcacatgat
      121 aggcatagat gatacctcca tccggatcgt tgttgttggc caggagatct ccaagcccga
      181 ccccgtaaaa gatattatcc cccagcacaa gagctacctt gtctttacct atgaaatcgg
      241 cacccaaagt gaaagcctgt gccagcccgt ccggactggg ttgttccaca taactgaagc
      301 tgcaacccag tctggaacca tcacccagca gcttctgaaa accgggcagg tcataaggag
      361 tggaaatgat caggatctcg ttgatgcccg ccatcatcag ggtactgagc gggtagtaga
      421 tcatgggctt atcatagatg ggcatcagct gcttgctgat ggccagggtg atcgggtgga
      481 gacgggtgcc ggatccaccg gccagtatga ttcctttcac agcaggtatt tgctgcaaat
      541 atacggaagg ctgccggagg gcttaaccga taagaggttt tcttggtctg gcatagtaaa
      601 gaatggccgt ggcgatccct gccaggatga acgcacccag ctgatgtacc acaccccaga
      661 ataccggaat atgggtggat gccagcagca gggtggtgac cccaagcagg aattgaagga
      721 acaccatcag tagcagtgca tgctccagcc tgcgcatgga tggggcactg aaacgacggt
      781 tctgcaacca gaatatcccg accgcgagga acaccaggat gcccagggta cggtggacga
      841 agttgatggt ggtggggtta tcaaagatcg ctgccatggc gttggctcca tgaaatgcat
      901 tgggggggat gaccgtgccg ttcatcaatg gccatgtctg gaacagcagg ccggctttgg
      961 tgcctgccat gaaaccaccg aatgcgatct gcacgacgat gatacccagc acccagcgaa
     1021 tgtgttttgc tgcaaccgga tggtctgtcg gaacaggttt cttgcgcatg gacagagcca
     1081 gccagatcag gtacagaaac accagcaaag caagcagg
//
LOCUS       DAOMAX010000503         1104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_42462_length_1104_cov_4.722593, whole genome shotgun sequence.
ACCESSION   DAOMAX010000503 DAOMAX010000000
VERSION     DAOMAX010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42462_length_1104_cov_4.722593"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PK742_14285"
     CDS             <1..368
                     /locus_tag="PK742_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQU77811.1"
                     /translation="QKALEMKNRYGVDGVMIGRASIGHPWIFREIRHFLETGEELATP
                     TIEERVSVCRAHLEKSIAWKGEVLGVLEMRRHYTNYFKGLPNVKHYRNLLVTEKEPQA
                     LFDVLNEIAQLSLENPVTV"
     gene            533..1093
                     /locus_tag="PK742_14290"
     CDS             533..1093
                     /locus_tag="PK742_14290"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQU77812.1"
                     /translation="MFLLIPLILIFLYGVFLYNSLIQKNNQVENAFGQVDVILKKRFD
                     LIPNLVETVRQYMTFEKDTLSRITELRSQLAGSPNMPAGQRIDTENELTRLLAGVRVA
                     VENYPELKTNESFSMLQRTWNEVENQLVGVRTSYNNTVTTFNNAVEMFPSNIMAGWMN
                     LNRKSVLVTADAERQSPNAKDLFKGE"
BASE COUNT          305 a          263 c          298 g          238 t
ORIGIN      
        1 cgcaaaaagc actggagatg aagaacagat atggtgtgga tggggtcatg ataggccggg
       61 ccagcatcgg gcatccgtgg atcttccgcg agatcagaca ttttctggaa accggtgaag
      121 aactggcaac tcccaccatc gaagagaggg tgtctgtgtg cagggcacat ttggaaaaat
      181 ccatcgcctg gaagggagaa gtgctgggtg tgctggagat gcgccgccac tacaccaact
      241 acttcaaagg cctgccgaat gtgaagcact accgcaacct gctggtcact gaaaaagaac
      301 ctcaggcctt gtttgatgta ctcaacgaga tcgctcagct gagtctggag aacccggtaa
      361 ctgtatgagt tgtttttcgc tgtttgtaaa tgtgaatgca tcaagctgac agcttcctta
      421 aatcagattc agtgagttgt tcaccctgaa tagcatgact gccaactcag aactgcagac
      481 tgaggactaa tacaacccat ccccttcgta tatttgacaa aacaactgaa ccatgttcct
      541 gctcatcccg ctcattctga tcttcctgta cggtgtattc ctgtacaaca gtcttatcca
      601 gaagaacaac caggtggaga atgctttcgg ccaggtggat gtcattctga agaagcgttt
      661 tgacctcatc cccaacctgg tggaaacggt caggcagtac atgacctttg aaaaagatac
      721 cctgagccgg atcaccgaac tgagaagcca gctggcagga tcacccaata tgcctgccgg
      781 acagcgcatt gatacggaga acgaactaac ccgtctgctg gcaggtgtgc gggtagcggt
      841 ggagaattac ccggaactga agaccaatga aagcttctcc atgctgcagc gcacctggaa
      901 tgaggtagag aatcagctgg tgggtgtaag gacctcctat aataatacgg tgaccacctt
      961 caacaatgcc gtagagatgt tcccgtcaaa tatcatggca ggctggatga acctgaacag
     1021 aaagagtgtg ctggtaaccg ccgatgcaga aaggcagagc ccgaacgcga aggacctgtt
     1081 caagggggag tgagagtggg agtg
//
LOCUS       DAOMAX010000504         1095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_43122_length_1095_cov_4.511538, whole genome shotgun sequence.
ACCESSION   DAOMAX010000504 DAOMAX010000000
VERSION     DAOMAX010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43122_length_1095_cov_4.511538"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PK742_14295"
     CDS             <1..560
                     /locus_tag="PK742_14295"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HQU77813.1"
                     /translation="KVLLPMEGKFAFSTGTIVVILAVLVSLYFIARWAIRRWQHTDLY
                     LRIRILFMNVRDGITSIRHLERFPLFIFYSVMIWLCYYAMIHICFFALPETTVYGVRE
                     GLAILVFGTLGIISTPGGIGAYQFIVTELLNHVYLLDRPVAYAFSWIVWVGQTIMIIL
                     FGLLSLLLLPVLSGKKQAHVEEGVH"
     gene            538..1035
                     /locus_tag="PK742_14300"
     CDS             538..1035
                     /locus_tag="PK742_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HQU77814.1"
                     /translation="MSRKAFIEHKIYADWKQLQHQLSVWRFSNRTIIFTNGCFDVLHP
                     GHFHLLNEAASMAEKPTVVVGLNSDASVSGLKGKGRPVHRFEDRALALASLFAVDAVI
                     GFDQDTPAELIDLVQPDILVKGGDYQISEIVGADKVLAAGGKVELVSYLSGHSTTTLI
                     SGDKK"
BASE COUNT          247 a          292 c          268 g          288 t
ORIGIN      
        1 aaaaggtgct gcttcccatg gaaggaaaat tcgccttcag caccggaacc atcgtcgtca
       61 tactggctgt tctggtatcg ctgtatttca tcgcccgctg ggccatccgc cgctggcagc
      121 acactgatct ctacctacgc atccgcatcc tgttcatgaa tgtgcgtgac ggcatcacct
      181 ccatccgcca cctggaacgt tttcccctgt tcatttttta ctctgtcatg atctggcttt
      241 gttactatgc catgatccat atctgcttct tcgccctgcc tgaaacaact gtatatggtg
      301 tgcgtgaagg cctggccatt ctggtgttcg gtaccctggg tatcatctcc acacccggtg
      361 gcatcggtgc ctatcagttc atcgtgaccg agctgctgaa ccacgtatat ctgctggaca
      421 gaccggtggc ttatgccttt agctggatcg tctgggtggg acaaaccatc atgatcattt
      481 tattcggact gctttcctta ttacttttac ccgtcctgtc cgggaaaaaa caagctcatg
      541 tcgaggaagg cgttcattga acataagatc tatgccgact ggaagcaact gcaacaccag
      601 ttgtcggtct ggcgcttcag caaccgtacc atcatcttta ccaacggctg ctttgacgta
      661 ctgcatcccg gccattttca cctgctgaac gaagcagcct ctatggctga aaaacccact
      721 gtggtagttg gtctgaacag cgacgcatcc gtgtccggat taaaaggcaa gggcagaccg
      781 gtgcaccgtt tcgaagaccg ggcgctggcc ctggcaagtc tctttgcggt ggatgccgtg
      841 atcggttttg atcaggatac tccggccgaa ctcattgacc tggtacaacc ggatattctt
      901 gtaaaaggcg gcgactatca gatcagcgag atcgttggtg cagataaggt cctggcagca
      961 ggcgggaagg tggaactcgt ttcttacctg tcaggtcatt ccactacgac actaatatca
     1021 ggagataaaa aataaatata tggaatttca attgacagat gaccatttaa tgatccgcca
     1081 ggcggcgaga gactt
//
LOCUS       DAOMAX010000505         1078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_44425_length_1078_cov_4.302053, whole genome shotgun sequence.
ACCESSION   DAOMAX010000505 DAOMAX010000000
VERSION     DAOMAX010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44425_length_1078_cov_4.302053"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PK742_14305"
     CDS             <1..556
                     /locus_tag="PK742_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQU77815.1"
                     /translation="ETSAPSNVLSATFQSLVLKAIYACPVGIYRMSPDIEDLVQTSNN
                     LARVEIKDGHASLLCLCRSSVDSEKYDMAQAITSALELAGCEVKLEGGYPGWAPAPHS
                     AIVTLMSDLYSELFDGKAHVNACHAGLECGILGTNYPGVDMISFGPNIRGAHSPDEKV
                     QISSVQKFWKYLLATLERIPEKAS"
     gene            559..>1078
                     /locus_tag="PK742_14310"
     CDS             559..>1078
                     /locus_tag="PK742_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77816.1"
                     /translation="MNKSKWTLLVLVMLFMLPQAKGQQAGLGMFRPAWFEQPVLYLYG
                     PVEYNKTSQDHTPVDSIVFQVEDGGGVNIISAPPYLVPEHLKLDYDVLWFRVIGITGG
                     FLEVIVNSTNGQTAYIRKDQGSFLYWPEFLTTISTVEPVAGNPLRIKPLDHASEVETE
                     FAFLHAVAARDEW"
BASE COUNT          266 a          246 c          284 g          282 t
ORIGIN      
        1 agaaacctct gcaccatcaa atgtgttgtc ggcaactttt cagtcgctgg tactgaaagc
       61 tatttatgca tgtcctgtag gaatctatcg tatgagtccc gatattgaag atctcgtgca
      121 gacttccaat aacctggcga gggtggagat caaagacggt catgcctcat tgctttgttt
      181 gtgtcgcagt tcagtggatt ctgaaaagta tgatatggca caggccatta catcagctct
      241 tgaactggcg ggttgtgaag taaaactgga aggcggatat cccggatggg ctcccgctcc
      301 ccactcagcc attgtcacat tgatgtcaga tctctacagt gagttgtttg atggtaaggc
      361 acatgtgaac gcctgtcatg caggtctgga atgcggcatc ctcggaacca attatcccgg
      421 tgtggacatg atcagctttg gtccgaacat ccgcggagcg cattcgcccg acgaaaaagt
      481 gcagatctcc agtgtacaga aattctggaa atacctgttg gctacactgg agaggatacc
      541 ggaaaaagca tcatgaaaat gaataagagt aagtggaccc tgctggtcct ggtcatgctg
      601 ttcatgctgc ctcaggccaa aggtcagcag gcaggtctcg gtatgtttcg tcctgcctgg
      661 tttgagcagc ctgtactgta tctgtatggc cctgtggagt acaacaagac ctcgcaggac
      721 catactccgg ttgactccat agtgtttcag gtagaagatg gaggtggagt aaacatcatc
      781 tccgccccac catatctggt tccggaacac ctgaaactgg actatgatgt gctgtggttc
      841 cgggtgatcg gtataacagg cgggtttctg gaagtgatcg ttaattccac aaatggtcag
      901 acagcctata tcaggaagga tcagggaagt tttctgtact ggccggaatt tctaactact
      961 atttcaacag tggagcctgt agcgggaaac ccactgcgca tcaaacctct tgatcatgcc
     1021 tcagaagtgg aaactgagtt cgcttttctg catgctgttg ctgcccgtga tgaatggt
//
LOCUS       DAOMAX010000506         1067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_45300_length_1067_cov_4.285573, whole genome shotgun sequence.
ACCESSION   DAOMAX010000506 DAOMAX010000000
VERSION     DAOMAX010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45300_length_1067_cov_4.285573"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..>1067)
                     /locus_tag="PK742_14315"
     CDS             complement(4..>1067)
                     /locus_tag="PK742_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77817.1"
                     /translation="FGGMDLRLTGFVAVLATGMWYACSNDLKINAPFAETTFVYGLLD
                     IGDSVHYLRIQRSFLNEDSTPYELIGDVYNYRYEEDELEVWVEAWKNDVQVATYPFEY
                     ILGDTVGLALEDGLFEPEANVLYRFTGQLDSTAEYRLFIHRWLQHDTITAVTKPVSSF
                     QVLFPVAFLYGINLTDTFDVIYVNTYATNAQIYDLVMRFRYREINLTTGDTSELFADW
                     PLATNQTGDNLNGLGVMAYEVKANLFYAFVAAELEVDPAVRREFVDLQYRFYAGGEEL
                     YWQYLNELATLGFQELYATTMYTNVRGGYGIFSSRRLQVSDSIQLSDQSLDTLACGYR
                     TFGLGFPSSTANPFYPGCE"
BASE COUNT          284 a          266 c          269 g          248 t
ORIGIN      
        1 cagctattca cagccaggat agaacgggtt ggcggtggag gaaggaaagc ccagtccaaa
       61 ggtccggtag ccgcaggcca gggtatcgag cgactgatct gacaactgaa tggagtcgct
      121 tacctgcaaa cggcgggagg agaaaatacc atatccgccc cgcacattgg tgtacatggt
      181 ggtggcatat aattcctgaa aacccagggt ggcaagttca ttcagatatt gccagtacag
      241 ttcttctcca cctgcataaa accggtattg cagatcaaca aattctctgc gcacagcggg
      301 atcaacttcc agctcagcag ctacaaatgc ataaaacaaa ttggccttca cttcataggc
      361 catgacaccg aggccgttga ggttatcgcc ggtttgattg gttgccagag gccagtcggc
      421 aaaaagctct gacgtatctc cggtggtgag gtttatttcg cggtacctga aacgcatcac
      481 caggtcatag atctgcgcat tggtggcata ggtattgaca tagatgacat caaatgtatc
      541 ggtcaggttg atgccgtata aaaatgctac cgggaataat acctgaaagg agctgacggg
      601 ttttgtaact gcggtgatgg tatcgtgctg cagccatcgg tgaatgaaca aacggtattc
      661 tgccgtgctg tccagctgcc ctgtaaaacg atacaataca ttggcctcag gctcgaacag
      721 tccgtcttcc agggccagcc cgaccgtatc gcccagaatg tattcaaaag gataggtggc
      781 aacctgtaca tcgttcttcc atgcttctac ccatacctcc agttcatctt cttcatagcg
      841 atagttgtac acatctccta tcagctcata tggggtgctg tcttcattaa ggaaggagcg
      901 ctgaatgcgc aggtaatgaa cagaatctcc aatgtccagc aggccataga cgaaagtggt
      961 ttcggcaaac ggcgcattga tcttcaggtc gttgctgcag gcataccaca ttcccgtggc
     1021 aagcacggcg acaaatcctg ttaaccgaag atccatgccc ccaaata
//
LOCUS       DAOMAX010000507         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_45400_length_1066_cov_3.927794, whole genome shotgun sequence.
ACCESSION   DAOMAX010000507 DAOMAX010000000
VERSION     DAOMAX010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45400_length_1066_cov_3.927794"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PK742_14320"
     CDS             complement(<1..325)
                     /locus_tag="PK742_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQU77818.1"
                     /translation="MSQSELIMPKMGESIMEATILKWLKKEGDAVALDEAVLEIATDK
                     VDSEVPSPFEGVISKILYQEGDVVEVGKPIAIISGEGGETVIEAQPIEKPVQQEAPVV
                     QETVVR"
     gene            complement(365..>1066)
                     /locus_tag="PK742_14325"
     CDS             complement(365..>1066)
                     /locus_tag="PK742_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HQU77819.1"
                     /translation="ESWLADRIADLEEALPPHITLAYLPSPGVVKLRLTGRGKDHRKL
                     QEEVDAQVHLFAKRLGKHVYGYDEETLMASIGRRLMALNKTLSLAESCTGGNIGKLIT
                     SIPGSSAYFMGSVVSYDNSVKEKVLGVSRATLDTHGAVSEETVQEMLSGILRITGTHY
                     AIAVSGVAGPGGGTEDKPVGTVYIGVAGPSVTEIKRYTFGQKRDTNIEYSSMYALHHL
                     RSMLDADLEKQGISA"
BASE COUNT          210 a          309 c          250 g          297 t
ORIGIN      
        1 ctctcacaac tgtttcctgt accacagggg cttcctgctg aacaggcttt tctatcggct
       61 gtgcttcaat gaccgtttca ccgccttcgc cgctgatgat ggcaataggc ttacctactt
      121 ccaccacgtc gccttcctga tacaggatct tgctgatcac accttcgaaa ggagagggta
      181 cttcactgtc caccttatcg gtggcgatct ccagcaccgc ttcgtccagg gctacggcat
      241 cgccttcctt cttcagccat ttgaggatgg tggcttccat gatgctctcg cccattttcg
      301 gcatgatcag ttctgactgc gacatatttc ggatttattt tctgttaaaa tagaggccac
      361 aaagttacgc tgaaatgccc tgtttttcaa gatcggcatc gagcatggaa cgcagatgat
      421 gcagggcgta catgctggaa tattcgatgt tcgtatcccg tttctggccg aaagtgtagc
      481 gtttgatctc tgtaactgag ggtcctgcca caccgatata gaccgttccc accggtttat
      541 cttcggtgcc gcctccgggc cctgccacgc cactcacagc aatagcgtaa tgggttccgg
      601 tgatgcgcaa tatcccgctc agcatttcct gaaccgtttc ttcactcact gctccatgag
      661 tatccagggt tgccctggaa acccccagca ccttttcctt gacactgttg tcatagctga
      721 ccacacttcc catgaaatag gcactgctgc ccggaatgga ggtgatgagt tttccgatat
      781 tgccacctgt gcagctctct gcgagcgaca gggttttatt cagtgccatc aaccgtcggc
      841 cgatggatgc catcagcgtt tcttcgtcat atccatatac gtgttttccc aaacgctttg
      901 cgaataagtg tacctgggca tctacttcct cctgcaattt cctgtggtct tttccccgtc
      961 cggtcagcct gagtttgacc acgcccggac tgggcagata ggccagagta atatgtggtg
     1021 gcagggcttc ttccagatct gcgatgcgat ctgccagcca ggactc
//
LOCUS       DAOMAX010000508         1066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_45401_length_1066_cov_3.859545, whole genome shotgun sequence.
ACCESSION   DAOMAX010000508 DAOMAX010000000
VERSION     DAOMAX010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45401_length_1066_cov_3.859545"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1066)
                     /locus_tag="PK742_14330"
     CDS             complement(<1..>1066)
                     /locus_tag="PK742_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQU77820.1"
                     /translation="GDTNIESIDITLMPGDNLIAFDDESGATPSEVSAYYHQNIVHDH
                     MKFYFPSFDDLDIAQLIRVERTDGSCNAFYDGTSTNFYAAGGGCPATALFRDVVYHEY
                     GHGINYDLYAFLGDPGGMNNGAMQEGYADIWGYTITNDPILGQGFSGGAGTYVRRYDA
                     EPKVYPFDLVGEVHADGEIIAGAWWDLAENLGDDIVAMTEIWAETILATVDGASGDEG
                     NIYRDVLLEALLADDDNADLSDGTPNDLAILEAFAEHGITLLADAEVTHAEALEPAAQ
                     DVGITIEANLAVSFPAYLGDFTMFYSTDAGETWSSVLMDAVTGLTYSADIPAQPVGTI
                     VEYYFVVSDIYGGIAVVDPKK"
BASE COUNT          278 a          277 c          243 g          268 t
ORIGIN      
        1 tttcttggga tcaaccactg caatacctcc gtagatatca gaaactacaa agtagtattc
       61 aacaatggtt cctaccggtt gtgccggtat atcagctgag taagtaagtc cggttacagc
      121 atccattaat acagagctcc atgtttcgcc agcatctgtg ctgtagaaca tagtaaaatc
      181 gcccaggtat gcagggaaag agactgccag gttagcttcg atagttattc caacatcctg
      241 agctgccggt tccagtgctt cggcatgggt cacttctgca tcagccagca gggtgatacc
      301 atgttctgca aaagcttcca ggattgccag gtcgttagga gtgccatcac tcaggtcagc
      361 gttgtcatcg tcagccaaaa gagcttccag caatacatcg cggtaaatat ttccttcatc
      421 tccgctggct ccatccactg tagccagtat ggtttcagcc cagatctctg tcatggccac
      481 aatatcatcg cccagattct ctgccagatc ccaccatgca ccggcaatga tctcgccatc
      541 tgcatgcact tcaccaacca ggtcaaaggg atacactttg ggttcagcat cgtatctgcg
      601 tacataggtg ccggctccac ctgaaaaacc ctgaccgaga atgggatcat tggtaatggt
      661 gtagccccag atatcagcat aaccttcctg catggctccg ttgttcattc cacccggatc
      721 gccaaggaat gcatagagat catagttgat gccatgaccg tattcgtgat agaccacatc
      781 gcggaacagt gcagtagcag ggcatccgcc accggcagca tagaagttcg tgcttgttcc
      841 atcatagaaa gcgttgcagg atccgtctgt tctctccacc ctgatcaatt gagcaatatc
      901 caggtcgtcg aaagaaggga aataaaactt catatgatca tgcacaatat tctggtggta
      961 ataggcagaa acctcagatg gtgtagcacc tgattcatcg tcaaatgcga tgaggttatc
     1021 gcccggcatc agggtaatat caatgctttc gatattggta tcgccg
//
LOCUS       DAOMAX010000509         1060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_45931_length_1060_cov_3.354229, whole genome shotgun sequence.
ACCESSION   DAOMAX010000509 DAOMAX010000000
VERSION     DAOMAX010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45931_length_1060_cov_3.354229"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1060)
                     /locus_tag="PK742_14335"
     CDS             complement(<1..>1060)
                     /locus_tag="PK742_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQU77821.1"
                     /translation="CRVRMGVQSHADYTDGDGNRVLNSRADGVQAKLSYGIRKKHFSS
                     DNHYYGSYNRFGFIFADLPYRMETDERWSRGYSGPHHMVMLHSLSSVNKFDLHASTLR
                     INAGVQSNYRAEDEGGGELSLQMHLATFQYALKWNKPITEKWLFILANNSSWEVNTNL
                     GRRKIVPDALMAEASSSGYIRYMEQHWLVEAGIGGGSRQITTLLTPTVNSDEKDIDPF
                     SQLRLFGNMMLGFSWMPDAMQELKLDVATGVRAPNLAELSSNGLHEGTYTYELGDPEL
                     LNERNIQADAGYHLHTDVLDLELAAFVNHFSGYIYLQPADYEWFGFPVAEFVQAPALL
                     DGGEAGISKAWQQWTFGLS"
BASE COUNT          256 a          292 c          255 g          257 t
ORIGIN      
        1 gctgagaccg aaggtccatt gttgccaggc cttgctgata ccagcttccc ctccatccag
       61 cagtgccggt gcctgcacaa attctgccac cggaaaaccg aaccattcat agtcagccgg
      121 ttgcaggtag atgtagccgg agaaatggtt gacaaatgct gctagctcca ggtccagtac
      181 gtcggtatga agatgatagc ctgcatcggc ctgtatattc ctttcattca gcagttcagg
      241 atcgcccagc tcgtaggtgt aggtgccttc gtgcagacca ttggacgata attcagccag
      301 gttcggtgca cgcacaccag tggccacgtc cagtttcagt tcctgcatgg catcaggcat
      361 ccaggaaaat cccagcatca tattgccgaa caagcgcagc tgactgaagg ggtcaatatc
      421 cttctcgtca gaatttaccg taggtgtcag caaggtggtg atctgtctgg atccaccgcc
      481 gataccggct tccaccaacc agtgctgctc catataccgt atgtaaccgg atgagcttgc
      541 ctctgccatc aacgcatcgg gtacgatctt ccgccttccc agattggtat tgacctccca
      601 ggaactgtta ttcgccagga tgaacaacca cttttctgtg atgggtttat tccatttgag
      661 tgcatactga aaggtggcca ggtgcatttg taaactgagc tctccgccac cttcatcttc
      721 ggcacgatag ttcgactgca caccagcgtt aatacgcagg gtagatgcat gcaggtcgaa
      781 cttattgaca gaagataaac tatgcagcat gaccatgtga tgcggaccgg aatacccacg
      841 actccaccgc tcatcggttt ccatcctgta aggcagatca gcgaagatga aaccaaatcg
      901 gttgtaggaa ccgtaatagt ggttatcgga actgaaatgc tttttacgga taccatatga
      961 cagttttgcc tgtacaccat cggcacgact gttgagcacc cggttaccat cgccgtcagt
     1021 ataatcggca tgactttgca ctcccatccg cacccggcac
//
LOCUS       DAOMAX010000510         1042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_47495_length_1042_cov_3.848024, whole genome shotgun sequence.
ACCESSION   DAOMAX010000510 DAOMAX010000000
VERSION     DAOMAX010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47495_length_1042_cov_3.848024"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PK742_14340"
     CDS             <1..865
                     /locus_tag="PK742_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQU77822.1"
                     /translation="HQAGLTPWIPFYKQTLLADGSLDPRYYNQAKIPGFTIKVADNIY
                     MRDDYRDSIWAQITQTPLKTKGSYKYSDLTMYIGKRIVEQLSGMTLDEYVYRNFYTPL
                     GMERTCYNPLERFQKKEIVPTENDNYFRYQTVHGYVHDMGAAMMGGVEGHAGLFSTAD
                     DLSVLFTMLLNGGSYGGVQYIQPETVELFTKKQSRVSRRGLGWDKPETDPSKSSPAGK
                     YASAATYGHQGFTGICVWADPVNDVLFVFLSNRVQPTADPNMLSREDIRNRMMDVVYE
                     SFMEEKGPAGK"
BASE COUNT          283 a          261 c          282 g          216 t
ORIGIN      
        1 ccaccaggca ggactcacgc cgtggatacc attctataag cagacactgc tggctgatgg
       61 ctcgctggat ccgaggtatt acaaccaggc aaagatcccg gggttcacca tcaaagtggc
      121 cgataacatt tacatgcggg atgactaccg cgacagtatc tgggcgcaga tcacgcagac
      181 accgctgaaa acaaaagggt catataaata cagcgacctc accatgtaca tcggtaaacg
      241 catcgtggaa cagctgagtg gaatgaccct ggatgaatat gtgtaccgga atttctatac
      301 tccactgggt atggaacgca cctgttacaa tccgctggaa cgttttcaga agaaggagat
      361 cgttcctact gagaatgata actacttccg ctaccagacc gtgcatggct acgtgcacga
      421 catgggtgca gccatgatgg gaggtgtgga aggacatgca ggattattca gcacggccga
      481 tgacctctcg gtcctgttca ccatgctgct caacggtggc agctacggag gtgtgcagta
      541 tatccaaccg gaaacagtgg agctcttcac taaaaaacag agccgtgttt cccgccgcgg
      601 attaggttgg gacaaacccg agaccgatcc gtccaaaagc agtccggcag gcaagtacgc
      661 cagtgctgcc acttacggac atcagggttt cacgggcatc tgcgtctggg ccgatccggt
      721 caatgatgtg ttgttcgtct tcctttccaa ccgggtgcag ccaaccgcag atccaaacat
      781 gctcagccgc gaagacatcc gcaaccgcat gatggatgtg gtctatgaaa gtttcatgga
      841 agagaagggg ccggcaggaa aataagaggc ataatgagat catcagagaa tgaggtattg
      901 ggtggttgcg aaaacaacgt gtgatgacga tcaggaatgg agaaatgcta caatgagccg
      961 gccaatacta atttgaagag cccgcccatt cacctttcac ccctcacttt actgcgagtg
     1021 agtggtgaaa cctggctatc gg
//
LOCUS       DAOMAX010000511         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_49509_length_1020_cov_3.789637, whole genome shotgun sequence.
ACCESSION   DAOMAX010000511 DAOMAX010000000
VERSION     DAOMAX010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49509_length_1020_cov_3.789637"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PK742_14345"
     CDS             <1..652
                     /locus_tag="PK742_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQU77823.1"
                     /translation="VQIYGGYGFSEEYPLARAYRDARINRIFEGTNEINRLLIVDMLL
                     KRAMKGQIDMMTPAMEVQKELMGIPEMGEPDTDAFSAEVKAIANMKKAILMVAGAAVQ
                     KLMMQLKDEQEILMAVADMIIDTFMAESALLRTMKHAESEGAAEREAMVQVLITDAMD
                     RINKSGKTAIMSFATGDEQRMMLLGLKRFTKYRMLNTTALRRTVAARLIEADNYCY"
     gene            complement(716..>1020)
                     /locus_tag="PK742_14350"
     CDS             complement(716..>1020)
                     /locus_tag="PK742_14350"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQU77824.1"
                     /translation="GVFIADLSGEVGFHSPDMDNQYEVYPNPAVNDLNILTSYSNGVE
                     QIQIVNLVGKVMLVQQWNKGTGKLVLDISNLPEGIYFVQFIGKDQVLKTKKISITR"
BASE COUNT          261 a          244 c          273 g          242 t
ORIGIN      
        1 cgtgcagatc tatggtggct atggtttcag cgaagagtat ccactggcac gtgcctaccg
       61 cgatgcacgt atcaaccgca tctttgaagg caccaacgag atcaacaggc ttctgatcgt
      121 agatatgctg ctgaagcggg ctatgaaagg ccagatcgat atgatgacgc ctgccatgga
      181 agtacagaag gaactgatgg gaataccgga gatgggtgaa cccgataccg atgcattttc
      241 ggctgaggta aaagccatcg ccaatatgaa gaaagcaatt ctgatggtgg ctggtgccgc
      301 tgtgcagaag ctgatgatgc agctgaaaga cgaacaggag atccttatgg cggtggccga
      361 tatgatcatc gacaccttca tggctgaatc tgcgctgctt cgcactatga agcatgcgga
      421 aagcgaaggt gctgccgaaa gagaagccat ggtgcaggtg ctgatcaccg atgcgatgga
      481 ccgtatcaac aagtccggta agacggccat catgagtttc gccaccggcg atgagcagcg
      541 catgatgctg ctggggctga agcggttcac caaataccgc atgttgaata ccactgcatt
      601 gcgacgcacc gttgcagcca gactgataga ggcagacaac tactgttact gatcaaaccg
      661 gaaaacaaaa aggcgcttct atgcagaagc gcctttttgt tttgtgatgg tgttgttatc
      721 tggtgatgct gatcttcttg gtcttcagca cctggtcttt accaatgaac tgcacgaagt
      781 agataccctc cggcaggttg ctgatatcca gtaccagttt accggtgcct ttgttccact
      841 gttgtaccag catgaccttt ccaaccaggt tcacgatctg gatctgctct actccgtttg
      901 aataggaagt cagaatattc agatcgttta cagcagggtt cggatagacc tcatactggt
      961 tgtccatgtc tggcgaatga aagcctactt caccgctcag atcggcgatg aataccccac
//
LOCUS       DAOMAX010000512         1020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5121
            NODE_49510_length_1020_cov_3.555440, whole genome shotgun sequence.
ACCESSION   DAOMAX010000512 DAOMAX010000000
VERSION     DAOMAX010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867280
            Sequence Read Archive: SRR1055898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 16:24:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,871
            CDSs (total)                      :: 2,834
            Genes (coding)                    :: 2,830
            CDSs (with protein)               :: 2,830
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49510_length_1020_cov_3.555440"
                     /isolate="AMR_MDS_5121"
                     /isolation_source="Foam and foaming activated sludge"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2012-03-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="PK742_14355"
     CDS             <1..>1020
                     /locus_tag="PK742_14355"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQU77825.1"
                     /translation="PPVIPVVEDTLVSIPIPQTDSLEIAAFLRAHTDWSAGNDLQEYY
                     GKRDFRFAWFNEYGLREEAGFFLNLVQHIGEEGISDTAKFFHETEVLFTQLGGTDYAY
                     VGKDSLNLQLELLLTGQFFAYADRVWNGLSESTSRSLQWYIKRKQLTYGDLLDSLLVN
                     GPSAFGAGIHMIAQYDLLRHALESYTALGDTTWPLISPGIDKTKLVLGDSSAEVLSIQ
                     ERLLVLGDLDSLENPGVFDQATDLAVRKFQYRHGLVEDGEAGKAFFGQINISPKDRAL
                     QIRANMERCRWVPSEPQGDYIMVNIPEFKMHVYEHDSLVFSMNTIVGKTSSSTIIFND
                     ELEYIV"
BASE COUNT          241 a          239 c          296 g          244 t
ORIGIN      
        1 ccgcctgtta ttcctgttgt ggaagacacg ttggtgagca tccccatacc tcaaacggac
       61 agcctggaga tcgcagcctt tctgcgggcg cataccgact ggtcggccgg aaatgatctg
      121 caggaatact atggcaaacg ggattttcgt tttgcctggt tcaatgaata cggactgcgg
      181 gaagaggcag gtttcttcct caacctggtg cagcatatcg gggaagaagg catcagtgat
      241 actgcgaagt ttttccacga aacagaggtg ttgtttactc agctgggggg aaccgactat
      301 gcctatgtgg ggaaggacag cctcaacctg caactggaat tgttgctcac cggacagttc
      361 ttcgcttatg ccgatcgtgt gtggaatggc ctgagtgagt ccaccagccg atcgctgcag
      421 tggtatatca agcggaaaca actgacctac ggcgacctgc tggattcgct gctggtgaat
      481 ggtccgtctg cctttggagc aggcatacat atgatcgccc agtatgacct gttgcgacat
      541 gcactggaaa gttatacggc tctgggagat accacctggc cgctcatcag ccccggcata
      601 gacaagacga aactggtgtt gggcgacagt tctgctgagg tactttccat ccaggaaaga
      661 ctgctggtat taggtgatct ggacagtctg gagaatcccg gtgtgttcga tcaggcgacg
      721 gatctggcag tgcggaaatt ccagtaccgg catggactgg tggaggatgg cgaagcggga
      781 aaggcattct tcgggcagat caatatttct ccgaaggatc gtgccttgca gatccgggcg
      841 aatatggaac gctgccgatg ggtgccctca gaaccacagg gtgattatat catggtcaat
      901 attccggagt tcaaaatgca tgtgtatgag cacgacagcc tggtgttcag catgaatact
      961 atagttggta agaccagttc ttctactatc atcttcaacg acgagctgga atacatcgtg
//
